LOCUS       DACULQ010000001        65621 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.001, whole genome shotgun sequence.
ACCESSION   DACULQ010000001 DACULQ010000000
VERSION     DACULQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 65621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.001"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..232
                     /locus_tag="JA154_00005"
     CDS             <1..232
                     /locus_tag="JA154_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994236.1"
                     /translation="VSSLSILLILIFENNVFKKVSKKFNYCILMIVAIDILSKKIKYE
                     KITARTIFSYLIFNIYIIQNFHLFNLEFENHY"
     gene            631..1653
                     /locus_tag="JA154_00010"
     CDS             631..1653
                     /locus_tag="JA154_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HAT7994237.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            1950..2870
                     /locus_tag="JA154_00015"
     CDS             1950..2870
                     /locus_tag="JA154_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAT7994238.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            2909..3601
                     /locus_tag="JA154_00020"
     CDS             2909..3601
                     /locus_tag="JA154_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAT7994239.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            3594..4496
                     /locus_tag="JA154_00025"
     CDS             3594..4496
                     /locus_tag="JA154_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAT7994240.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            4581..5165
                     /locus_tag="JA154_00030"
     CDS             4581..5165
                     /locus_tag="JA154_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAT7994241.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            5380..6306
                     /locus_tag="JA154_00035"
     CDS             5380..6306
                     /locus_tag="JA154_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase"
                     /protein_id="HAT7994242.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(6358..7248)
                     /locus_tag="JA154_00040"
     CDS             complement(6358..7248)
                     /locus_tag="JA154_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT7994243.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            7436..8467
                     /locus_tag="JA154_00045"
     CDS             7436..8467
                     /locus_tag="JA154_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAT7994244.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            8761..9636
                     /locus_tag="JA154_00050"
     CDS             8761..9636
                     /locus_tag="JA154_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HAT7994245.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            9654..11144
                     /locus_tag="JA154_00055"
     CDS             9654..11144
                     /locus_tag="JA154_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAT7994246.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            11337..11621
                     /locus_tag="JA154_00060"
     CDS             11337..11621
                     /locus_tag="JA154_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAT7994247.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            11654..13282
                     /gene="groEL"
                     /locus_tag="JA154_00065"
     CDS             11654..13282
                     /gene="groEL"
                     /locus_tag="JA154_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086664.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAT7994248.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDVGMPGMGGGMPGMM"
     gene            13544..14284
                     /locus_tag="JA154_00070"
     CDS             13544..14284
                     /locus_tag="JA154_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-amino-4-imidazole-
                     carboxylate carboxylase"
                     /protein_id="HAT7994249.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            14368..15102
                     /locus_tag="JA154_00075"
     CDS             14368..15102
                     /locus_tag="JA154_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00299 family protein"
                     /protein_id="HAT7994250.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            15132..15551
                     /locus_tag="JA154_00080"
     CDS             15132..15551
                     /locus_tag="JA154_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994251.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      15611..15712
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15933..17468
                     /locus_tag="JA154_00085"
     CDS             15933..17468
                     /locus_tag="JA154_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HAT7994252.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(17545..18591)
                     /locus_tag="JA154_00090"
     CDS             complement(17545..18591)
                     /locus_tag="JA154_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT7994253.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(18668..18883)
                     /locus_tag="JA154_00095"
     CDS             complement(18668..18883)
                     /locus_tag="JA154_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994254.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
     gene            complement(19317..19718)
                     /locus_tag="JA154_00100"
     CDS             complement(19317..19718)
                     /locus_tag="JA154_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7994255.1"
                     /translation="MQTLAEIIKEYRSEHNLSLREFSNLCGVSHTYIDKIEKNRDPRN
                     GKAVEPTLDMLEKMSFGLNLTLKELLTKLGKIQPNTQQDDELLKKVNSKEDNEVNELI
                     NRLASLDSDDKNAIKKMIDNAYYKAVNTKKE"
     gene            19883..20095
                     /locus_tag="JA154_00105"
     CDS             19883..20095
                     /locus_tag="JA154_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7994256.1"
                     /translation="MKNNLQYIRKESRISQKKFADKIGISRPYLSRIENGKVNPSLEI
                     AHKISLETGKTINEIFFDFTVNHSKL"
     gene            complement(20390..20608)
                     /locus_tag="JA154_00110"
     CDS             complement(20390..20608)
                     /locus_tag="JA154_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7994257.1"
                     /translation="MTKFKDNIKLVRKQMNMTQKQFASLFGISERAYQYYESGSREPN
                     IETLILISNKLNVSTDFLLGLSPNQNRN"
     gene            20727..20915
                     /locus_tag="JA154_00115"
     CDS             20727..20915
                     /locus_tag="JA154_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7994258.1"
                     /translation="MNNNLKLQREKVGLTQLEVAQKAKITERSYQYYEAGERLPNIRT
                     ALKIARILNTNCEKLFNE"
     gene            20957..21751
                     /locus_tag="JA154_00120"
     CDS             20957..21751
                     /locus_tag="JA154_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAT7994259.1"
                     /translation="MNNLQLNNKNTITTLEIADMLEINHWEVLRKLEGTEKTKGIIDI
                     LNDNNFVVVDYFIKSAYLDLKNENRKCYNVTKLGCDFLANKFTGEKGIIFTARYVKRF
                     NKMEQELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADS
                     VASSDNSILVGELAKLLRQNGIDTGQNRLFDWLRNNGYLIKRKGEDYNTPTQKSVDLG
                     VIETKEGTRVHPDGHTSITKTPKITGKGQIYFINKFKKNNQISMLG"
     gene            21835..22215
                     /locus_tag="JA154_00125"
     CDS             21835..22215
                     /locus_tag="JA154_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994260.1"
                     /translation="MKNNTSDSRVKYFCKCPYCGFDNEVEVKKGSKPKICCICTKEVE
                     YEKLEQQSVLENTEIKEVCNQMKIKKLALATGEVFENIELKEFENKGECLPPNMVKVI
                     DGKQELLINKEFILSLEVKRKTTF"
     gene            22416..22613
                     /locus_tag="JA154_00130"
     CDS             22416..22613
                     /locus_tag="JA154_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7994261.1"
                     /translation="MAINDNINKILRDRDLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKLADYLDVTLDELVGR"
     gene            22652..22858
                     /locus_tag="JA154_00135"
     CDS             22652..22858
                     /locus_tag="JA154_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994262.1"
                     /translation="MAKAVAKEQLFYRAKDIAKILDVCEATAYKIIGELNKELEKEGW
                     KTFPGRVSVTYFKERYCYKTKKGA"
     gene            23030..23257
                     /locus_tag="JA154_00140"
     CDS             23030..23257
                     /locus_tag="JA154_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994263.1"
                     /translation="MKKLKVKFPTKILKAGKAIRVTCARFGFESDCIITQSQEFELTV
                     VYFDKELDDLVQSSITIDDAISYDYCIETLN"
     gene            23271..23588
                     /locus_tag="JA154_00145"
     CDS             23271..23588
                     /locus_tag="JA154_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994264.1"
                     /translation="MKSLIIVRNSVEQQLNRANLEINKNEQLYTKLRKKEERNISEEI
                     ELSNALREKSVNERLKIFAESLLKIIDTQIEIKEYEESEDYKIFELISEELERDIPID
                     VQI"
     gene            23671..24180
                     /locus_tag="JA154_00150"
     CDS             23671..24180
                     /locus_tag="JA154_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994265.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRAKKNTLDKLKSNIKDCMELLETKKVETIL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            24190..24798
                     /locus_tag="JA154_00155"
     CDS             24190..24798
                     /locus_tag="JA154_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAT7994266.1"
                     /translation="METNNVYIKLVNIQSTLKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVVLDDNIVQIGNRFYVEATATLIDAETGEKISVKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNILYSLGESIEK
                     DKNRVDSEVYKKFGKLAVDLTKQEYEKVLNGYKSILEKQKQE"
     gene            24808..25689
                     /locus_tag="JA154_00160"
     CDS             24808..25689
                     /locus_tag="JA154_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAT7994267.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLYKRSKNGRDATANILRELIENGYITRTPCRDSNTNKMLGGYDYQVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAIDEAIANGVKNYKYVKTILNN
                     WIEAGVKTNLELTEYQNEFERKKKNKQDKKQSNSKAVNTPNVSKNKFHNFNETFTQYS
                     PDELDDIIKKSQKVKFK"
     gene            25750..25923
                     /locus_tag="JA154_00165"
     CDS             25750..25923
                     /locus_tag="JA154_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAT7994268.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            25939..26346
                     /locus_tag="JA154_00170"
     CDS             25939..26346
                     /locus_tag="JA154_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAT7994269.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKAKPVYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
     gene            26666..26917
                     /locus_tag="JA154_00175"
     CDS             26666..26917
                     /locus_tag="JA154_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994270.1"
                     /translation="MIIIRSQDRLDLMRVNRVEIYDKQVFVIFEDDVKRIGVYESNER
                     AIEVLNRIQEAIIAGTKFDIINKDGVRCNKEKVFEMPVE"
     gene            26931..27296
                     /locus_tag="JA154_00180"
     CDS             26931..27296
                     /locus_tag="JA154_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994271.1"
                     /translation="MLKVEKYFNGSVADNIFEDDLTLRNYLALYCCVYGVRKNGELVF
                     PTSEKMLIEFNVDKNRKKKKKGSKVKLINAKTGEEKIFDSIDSAACFLRLQSQAVYQT
                     IKKKTKTRSGWKAEYIKEE"
     gene            27414..27863
                     /locus_tag="JA154_00185"
     CDS             27414..27863
                     /locus_tag="JA154_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994272.1"
                     /translation="MSLIKYRGYDFENEKWIYSATIMWSNILECLVMLTEGCKWQKVS
                     NVGVCSGEWARNNQEICEGDILKGYDNSSDTSQYGVVKRDFNSIKLYLEWHYLKKFEG
                     EWIELINKTEIYHSRDYKVIGNEYENLEEVRTEFLERKESLENEYLS"
     gene            27847..27963
                     /locus_tag="JA154_00190"
     CDS             27847..27963
                     /locus_tag="JA154_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994273.1"
                     /translation="MNILASAILVIGSFITGRVYEYRLNLKECENCDNKRGV"
     gene            28252..29325
                     /locus_tag="JA154_00195"
     CDS             28252..29325
                     /locus_tag="JA154_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAT7994274.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKVFPIERKSGKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGG
                     GGAKTGLYFIDLNKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQ
                     NGRRIKESGETMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDS
                     QLYKQAGNAVTANVVYEIAKRMG"
     gene            29405..29614
                     /locus_tag="JA154_00200"
     CDS             29405..29614
                     /locus_tag="JA154_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994275.1"
                     /translation="MYEYILRWQIGLSLENRKIHYTYGSKEALRKKAKALAKDENIVL
                     ITIDKVDEVIKNTISEKIIERFENL"
     gene            29626..30630
                     /locus_tag="JA154_00205"
     CDS             29626..30630
                     /locus_tag="JA154_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAT7994276.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVNQDMVLFFQKKISKVSR
                     DNDIRTAVFNNYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNNSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDKYHLRVLDKDAEKEEANSKFVTEFLNATKIKDDKYKTKKFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLREKHEIDINDFVDKTIKDDKLKDSFKEHMEEKGLVEGFSI
                     DKKWVDKKLKKRNIKTDNGFEIKGNLTDFEDPMKYTVRQNQNGSIDIVIKNVTFYEEK
                     "
     gene            30712..31041
                     /locus_tag="JA154_00210"
     CDS             30712..31041
                     /locus_tag="JA154_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994277.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNDTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            31078..31449
                     /locus_tag="JA154_00215"
     CDS             31078..31449
                     /locus_tag="JA154_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994278.1"
                     /translation="MFNIYKVKIKTKRTLEQVRNQSVDFEYSEKGLKNTLKYYNLIDD
                     LKVIVVKFGDEYCLANYNEEDRKIIMEAHYLLEQDEYTGCYINEYERFKKDWENGNCD
                     GEACMVFSDDEIEIIEKLREG"
     gene            31452..31634
                     /locus_tag="JA154_00220"
     CDS             31452..31634
                     /locus_tag="JA154_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994279.1"
                     /translation="MNKRIKMKKRLIHKKCDERCVNYDFVISNNLITCNVCIGCKYKE
                     NMDKVCEENYKKLRSK"
     gene            31709..32050
                     /locus_tag="JA154_00225"
     CDS             31709..32050
                     /locus_tag="JA154_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HAT7994280.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNFKECESYLEGKNELYE
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEELTK
                     INSHLELEDLR"
     gene            32066..32179
                     /locus_tag="JA154_00230"
     CDS             32066..32179
                     /locus_tag="JA154_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994281.1"
                     /translation="MKRRRCSWCGKLFYLEEKSKDVYCCKECRKKAKKVKK"
     gene            32176..32583
                     /locus_tag="JA154_00235"
     CDS             32176..32583
                     /locus_tag="JA154_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAT7994282.1"
                     /translation="MKVFLVIDGEPVGKERPRMNSITKRIYTPNKTRDYEELIKWLYQ
                     SKVKYRFTGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDII"
     gene            32598..33323
                     /locus_tag="JA154_00240"
     CDS             32598..33323
                     /locus_tag="JA154_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAT7994283.1"
                     /translation="MNEIMTNENLRVVADDLVTVYETDTGEKIVFARELHNNLEVKRQ
                     FIDWIEDRIKQYGFKENEDYSVFHKNMKNSTGGRPSKEYVLKLDVAKELAMVQNNNKG
                     REIRRYFIRLEKLLNRTLSNSQLSQINTIVNESLLKMEVKHNIQIEQLKKECSEYYRP
                     TSKTKYDISSYIKERLGISKVNEEFELVKKRTLLVLGADKWEDIPKDVLLNSLNLIDE
                     SIRIIKSERKTNQISFFEKDNFC"
     gene            33417..33905
                     /locus_tag="JA154_00245"
     CDS             33417..33905
                     /locus_tag="JA154_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994284.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSSVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKIMDMLYPRIKENELPLF
                     KN"
     gene            34466..35143
                     /locus_tag="JA154_00250"
     CDS             34466..35143
                     /locus_tag="JA154_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HAT7994285.1"
                     /translation="MLKILQEKNVKVMWSKNGEEVWFNANDVGEELGIVNIRDTLRNI
                     DREYKKKFNESTVGDSYTRNFKDKLPNFGTTFVTEEAVYNMSFRSNKPEAKLFTKWVT
                     KTLKQIRIHGYYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVYYATSQGSNKPQMYY
                     KHFTELVRKKLGIPKGVKRDELNQSELFDIQALERIISMKLPKLIDKDMNYKEVYKKI
                     KELIEMI"
     gene            35206..35907
                     /locus_tag="JA154_00255"
     CDS             35206..35907
                     /locus_tag="JA154_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994286.1"
                     /translation="MQDVKEKVKQDYIKGMKQKEISAKYDISLNTLKSWIKRYNWASE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELIQEISIKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLKKSDTGDDSKIWV
                     EAIQNIAMKRGVNNG"
     gene            35900..37309
                     /locus_tag="JA154_00260"
     CDS             35900..37309
                     /locus_tag="JA154_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HAT7994287.1"
                     /translation="MDKALLTLLDCYWDNPVWFAEDMLNFKADKWQSDVLMALAQTPK
                     VSIRSGQGVGKTGLESIATVWYLSTRPFPKVVATAPTRQQLYDVLWAEIAKWLSNSKV
                     EKLLEWTKTKVYMKGFEERWWATARTAVKPENMQGFHEDYMLFVVDEASGVADPIMEA
                     ILGTLSGAENKLLLCGNPTRTSGTFYDSHNRDRDLYKTFKVSSLDSPRTSKDNIEMLK
                     RKYHEGSDPWRVRVLGEFPKGESDSLISLEAVETSTIREVNISNDYILNIGADIARYG
                     DDETIIAPRIGGKVFDLLTYSKKDTMETVGNILRAVDKFKSMYHQINRVKIKTDDDGL
                     GAGVTDRLKEVIRQERLKYEIVPIQNGSSAIEKDKYYNKASEMWDNMREELDANLSGF
                     IQNKEAIIQLPNDDKLIKQLSNRKYTVDSKGKIQIESKKEMKKRIGESPDRADAVIYS
                     FAENNNTDLSLLKGGSVWG"
     gene            37300..38802
                     /locus_tag="JA154_00265"
     CDS             37300..38802
                     /locus_tag="JA154_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAT7994288.1"
                     /translation="MGIISYVKKLFKRPTGEIMRMSSGNIGVYKLDDSRVDYELAREL
                     YQNKNANYKLGSSFVRPIVNSTTGFMGVPHFQIEDEEAQYILDEFVLDNTSKMLKTHT
                     DSLKQGDCYIWITREERENPLYPDKKVRLIYNFISPEEVKEIILDPTTKEPIAYILES
                     QNEWTDLEENKRRAKVKQIITAESRIIEVEGDKIEGLEEGETPNVWGFIPIIHFKNEA
                     DETLKYGQSDIEPIEPLLKAYHDVMLHALKGSKMHSTPKLKLKLTDVASFLAHNFGVE
                     DPVKFAKEGGKINLDGHEILFLNKDEEAEFVEVKSAIGDAKELLKLLFYCIVDVSETP
                     EFIFGVHTPSALASVKEQMPIMVNKIRRKREQFTNSWQLLARMVLIMSSNSSGMKYSS
                     YDVTIGWDEVNPRDDKELAETLEKVCSALDKALEGGFISEESTVNFLAQYIDTMSNYI
                     SDDPEIVGEREKIIKTKMLKYRLDDSQGLDDESNEIDKEIDKIKDNNDNG"
     gene            38795..39802
                     /locus_tag="JA154_00270"
     CDS             38795..39802
                     /locus_tag="JA154_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994289.1"
                     /translation="MDKSTLELITVAGEYKKWALEARKKFIDLRLKQDDEIRTMYINI
                     TRNITKEIRKGNLSDFNKVRLKQILKQLTQEIKILNEQLVFNFDEYLNKNVETATSYS
                     KNILINAVETAQITKVTKAMIQKAFYDINIRTVEAYYTRVKDGLFLSDRIWSKCKKYR
                     EDMKVILQTAVTEGQDCVKTAKMLDKYVLKGKKTLVDEYPNMIKRIGNRVPQNISYEA
                     LRLARTEMTSAYGDGVLASAMINPATIGIQFMLSMAHPHTDICDEICGEDNFGLGKGV
                     YPINEAPAYPFHPHCLCIMLTVVQPLDILVGRLKNWIKNPMNDTPLEMWYQEVYGNLN
                     F"
     gene            39822..41156
                     /locus_tag="JA154_00275"
     CDS             39822..41156
                     /locus_tag="JA154_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994290.1"
                     /translation="MNVITGEMDSMNALISSIKPSDIPLAKDIDIEALKSIDDDPLEV
                     VVEIPATKSKRGWNYTAKSLKDIVDYTNENTLNGFLGHQKAENISTEFAPPVTHWIGA
                     EMKGDKAYFRGLIDADATNLKRWIRTKRIKEVSIFGYPKLKKSAKGEMNVIGYEPLSI
                     DWTPLHRPGMPTSIVGMEMSPNGEQLDGTFEALRIDLREALKAKFSINDNNSYLYIQN
                     IRYDNNTVIYELEQNGLCKLYSIPFTIVENKINLGEEIEVIKKISYEAKGEMKGEENK
                     LEGKELIKNVKGLLQTGEISYSEVIQGIGLTKEIVTGEMEDVKSSLKAEKELREVKKV
                     LGIVGEMDTVEVAKKASKALENEKKEAWNCIVNKVIKDKVSGEIAQTLVKKMLNVEEG
                     SSEEVITGEIENILNDEFVKNTMSNMYKDNPTTTGLLNSSNNGSLTTKKNRI"
     gene            41172..41555
                     /locus_tag="JA154_00280"
     CDS             41172..41555
                     /locus_tag="JA154_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994291.1"
                     /translation="MAFKGQPTPSTITQITRAKISDGKSVRVILSEGESTKTQQFYLI
                     NGFFGVAMQDGEKGDEVTLQIEQAEYETDNIVTSEAFEAGKLIYWDNTAKKFTTTSAS
                     NRLVGRVTDGKDSNNVIWFILLPQQ"
     gene            41574..42611
                     /locus_tag="JA154_00285"
     CDS             41574..42611
                     /locus_tag="JA154_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAT7994292.1"
                     /translation="MAFKVISQENLLEQKRKETLQEDIPFIVNGEMEYVTKKISNGEM
                     ETLELNKPLGEMMTFSSTSNLKELLRKVVLDVELGREQVQLLYKPIYDSIADSNLPQV
                     MDAKWALQGNCVFLEHIEGEEIKFGTINAENGPVARIQTYATGFEYTKEMKDFNQTFS
                     VEILNKSIGESYNALLNHIHLSPIINFNYKASNKTAFKGETNDPIWLGIWRTLTQAQK
                     DTVIAKRQGNILMASSADQIEIEMALNGGHLLNGSMYPSIKNISTVIYYDGWEVTVGK
                     KTYSYKGVTPGKGYLIRPKRGFKELIKRDLTTEVGNADLSKLVENQIVGHCYRGAFAA
                     VEENVQEISFR"
     gene            42651..42989
                     /locus_tag="JA154_00290"
     CDS             42651..42989
                     /locus_tag="JA154_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994293.1"
                     /translation="MTPTSNLIEKLRLLLNDKDKKSFTDEELNLFLEEADCIYCAASQ
                     GWVLKSLQYENTVGEMYEYKVGQETYKSSSIKDLVSVAYQNADKFKDMCTNKKEKGSF
                     MLGISTEFEI"
     gene            42986..43363
                     /locus_tag="JA154_00295"
     CDS             42986..43363
                     /locus_tag="JA154_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994294.1"
                     /translation="MINIDRRRKDIIRTININPTNITITSIKKTEIDGAFEETETEIK
                     CVVRIFNEKTAEKQISSEKQGTFSSIRTYGMLVSDDVVLDVNSRDSLEFECIYGRMKI
                     VNVYPQIVKGELCGYQCSLERID"
     gene            43329..43775
                     /locus_tag="JA154_00300"
     CDS             43329..43775
                     /locus_tag="JA154_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994295.1"
                     /translation="MGINVHLKGLIKMSAFTNAINDINRKKAGMFVLCMSASAMLEGE
                     AKANARWTDRTSHARQSLNAKTLSGGNNFIIRLSHGAEYGGILEEGSKPHVITPKSAQ
                     ALYWRGASHPVKSVQHPGTKAMPIIKPTIDKNIGKIGNMIFRYWSD"
     gene            43776..44597
                     /locus_tag="JA154_00305"
     CDS             43776..44597
                     /locus_tag="JA154_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994296.1"
                     /translation="MRAGIRKALIDNIKELKGCYEPNVPNKDTKKPYMVVVQGQDNDN
                     GETIGFERSIEVWIYEGRTTFKKLDKLTKQVIEILDMNTIVDESENEAFTCIYKGTSE
                     NDIVVEEWDAIARGIRFSVIALEDKEDTTNDRWVEALSRHTKDLLEIESYKDNWKKNF
                     IAPCALWRTTHIENKRINYHLIEITKTMKCHVVSKNKDEIVKLLETLETSLIIDKRVR
                     LREDKNMYLTLVSVVEDRESDMFTTGQLTAVFKMIGKIKREGPTMDKIYGNGNLR"
     gene            44610..44813
                     /locus_tag="JA154_00310"
     CDS             44610..44813
                     /locus_tag="JA154_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994297.1"
                     /translation="MAETNNKKINVSKQEEKYLKSDFIENSEALGYKKEVVAGALFNC
                     KKEELTKSEFEKAIKEFLEREVK"
     gene            44815..46236
                     /locus_tag="JA154_00315"
     CDS             44815..46236
                     /locus_tag="JA154_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HAT7994298.1"
                     /translation="MATGTWNEKEKKEIPGFYNRFKTQAEKSTNTGLKGRLAMPIRAN
                     WGEVGKVVTIKNDLRQLKTLFGDDMNYSAFKLGKLALLGNVKELLLYRLVDGNQKKGT
                     LTLKDTTENSAKDVIKLETKYPTSRNFNVTIKSNLVDADKKDFIFFEGTKQLFSSSVK
                     GTIDEIVLEINSNLDNEYVIATKVADSDTALANLVNVALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVAEEALQETTKAWVAKNKELGKDILLFLGGKAEDNIKQINDKS
                     KSFNDENIVNVGSSAYYEGIKYTPSEVAVYIAALSVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLILDFDDGDVIIVDDVNTFKKYVDDKNEAIGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDSTGQTTVICALKKYFEELMGQGIISEFNVDIDTELQATAKADE
                     FYWKWDAVKVDVMKKIFGTGYLG"
     gene            46252..46674
                     /locus_tag="JA154_00320"
     CDS             46252..46674
                     /locus_tag="JA154_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994299.1"
                     /translation="MGKYDENIIDAANVADGSNAKIIVDGKEEGYGTEFTAEVENDKK
                     TFRVIGCKWELNKASTQKGTFSLTVLKTTSEWIERGFDKFELISEIENPGLVGYERIR
                     YKNCMVDKIQLASIKADENIEIQIDGTFEGYELVDKIA"
     gene            46753..47226
                     /locus_tag="JA154_00325"
     CDS             46753..47226
                     /locus_tag="JA154_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994300.1"
                     /translation="MANLDKEFLNEGIEEERELTKEEIAKQQEDNIIMKLTEDAILPE
                     KTIFVKRLDIPLTLRALTEKEISALQKKYTKVTKVRGRRESKLMEDEFNIALIEKATI
                     VPNFCDARLLNSMNVSSGVEFIRRKFLAGEIALISDEVLELSGFYEELSDDDIKN"
     gene            47407..51735
                     /locus_tag="JA154_00330"
     CDS             47407..51735
                     /locus_tag="JA154_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAT7994301.1"
                     /translation="MREIAKKEMYHIDVVIDVTGDEQTKNKLSAMERYTKQTEKRMRA
                     LNRIKANPVIQAQDKTSSVVNRISNNLKRVGRTISTTINAKDRASSVVNRVKNKVNSL
                     LTSRQREVLLKARDKASQVVDKVKAKVQNLTAATIISLNMKADPALRVISQTRSKLGE
                     LKNNTIINIKAKGEEALNTISRTKSKLQEFSNRTYQAIVKLKDEASPTLSGLDGKISS
                     FISSTISKFTQLAVTATALIGGVGVGSAIKGFADFEQAMKNAQAVSSANSKEMAEMTA
                     MAREMGRTTSFTAKDAGNAMYFMGMAGWKSKEMIAGLPGILNLAATGQTDLALTADIV
                     TDGLTALGLTAKDTGMFVDVMAATVTNSNTDIERMGETFKYMGSVGGALGVSMKDLSL
                     ATGLMASASVKGSMAGTALRGGLVRLIKPPAEAQKAMNKYGIEIKKTKDGNLDLASTI
                     VDLREKLGGLEGVQKSAAISSIFGRTAMAGWAAVVNASEKDFKKLTTAINESEGEAKR
                     IADMKLDTLSGQFEILKSAIDDVRISVGQRLGPMTRSFVEQLTKDMPKIGDAIVSFVS
                     DFINDFDKIKSIMQNVISVISGIVSAFIAFKALKFISFLIPILSDIGFAIAAFAGGAA
                     TLGEALLFALGGPVGAVIAGVVLLATAFTLAYQKSDAFRKIVKNVGKSIKNFLQEAII
                     AISPFVNVFGNKLKELGKAVIPLLKVFGDFASTLMSKIGPIILFLSSNVLAGFILTFV
                     AAVEAVKSAVVAITGVLQGLTSIIKGVFDIVGGIISGDGKQIINGLKSVFEGGIKIVS
                     SVWKGLVDIVTSPIQAVVDVLDEKFGKKVEGIKKKWNELKDFLKNPTKAAPKVQPVNL
                     SGEKASNELQASSNGAKAYISSLGQKIGEGIGKIKDKFGELKISATEVFNNIVSFVGG
                     KATELKDKLLEGIKPAIDTFKQSLSNLKEVFGDSLDSIKEAFGGLKTVFDENIKTPFE
                     NLKQKVLETKESLKPVFDNLKSSFAELGKALEPIKEAFSGIKDFFSNLFKPIKDDGAT
                     KTTKTNMDELKQSTQRVGTSFKELGNAFNQLKEAAKPFIDYLKQIKDSLTSTLGDIGG
                     GLLKGVATSIVLVITSVINAIASIINAVAGAIKGVIDIIKGIFEIIGGIISGDGEKIK
                     QGFSDVFKGIGEVVKSLWEGIKGVLGAPLKAVVNFIDNGFSEKVGQVKQWWSDLKTNV
                     GQKISGFVSFVSNGFQQKVQQVGMWWQGLKVNLSGKISGFVSLVENGFKSKVDSIKSA
                     WDSLRKKLSQKITGFVSIVKTGISNVLDRFADGGVASKPSICGEAGPEMVIPLSNSKR
                     SRALSLYEQAGQMLGTKASNNVIPISQKLGTSFNSASSIQNSNSSIINNVRQFPTKQE
                     EFNNTENRIYQEAQPQNIISSGSNAINVGGISINIQGSNNKEEMIQEILSQVESGLRE
                     ALEDIG"
     gene            51815..52609
                     /locus_tag="JA154_00335"
     CDS             51815..52609
                     /locus_tag="JA154_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994302.1"
                     /translation="MWGRFKNMSIILKVLVLVCAIAIFPITLLAFSIEFVVKSFKRNE
                     RFKVVFGVFLVFITFSFVCIWYLLEDNITTSNSSNKKQEEHLKEEPGKNLATREAEEE
                     TNKKKEQEKQKNEGTEAEKKERDKTEKKEKVDKKYQEKNKEKAETKKQTLTSEELKKK
                     VDSIIPAEYKGNYYTNDVLDTDGSYVLSLQVQNASFDNESSCKAFTKDLINKLKEFRI
                     DSAEVYFVGSSGQTTYQINIDDFLKVQDNIKSVDNMEFFSFKDFKN"
     gene            52679..53350
                     /locus_tag="JA154_00340"
     CDS             52679..53350
                     /locus_tag="JA154_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAT7994303.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVNPFDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPNLYDASYCRYSELKNPIEVVAMLEKWVDQAEPLRLIVT
                     GFGYNGLVTISNFSNTQTAGREEDRDIEITFRTYRELKIETLKKDTKSNTKTDLKDNR
                     PNTQTKSKIYTVKASDTLYKIAKNLLGKGSRWPEIYNIPENKKVIGKNPNIIKKGQKL
                     VIPSK"
     gene            53347..54309
                     /locus_tag="JA154_00345"
     CDS             53347..54309
                     /locus_tag="JA154_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HAT7994304.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDVSLVEPKQLKDI
                     NIKKGDKIILIDIAYESKKEETIFDGVVWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     QFKENTATQRIEYYCKQWNIPYYNLANTGKKLAKVIHKTNILDMIKKDLKETATKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDADKFGTLQKIKQDEKIKNAKEAKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKSKEYYVIDVTHTLDSRPKMKLNIGSLEYIRRKFYNND"
     gene            54302..54667
                     /locus_tag="JA154_00350"
     CDS             54302..54667
                     /locus_tag="JA154_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994305.1"
                     /translation="MTDARFNGIARILKENMNKSVANGTFGMGCELAEITANGLKVNG
                     YKDEIQDYLVLENLTLKEDYFTFSDEALSGEYRHKHKIETPKELKPLRIGDNVLVAVM
                     GAEFVVIGRVVNAKPISSK"
     gene            54642..55100
                     /locus_tag="JA154_00355"
     CDS             54642..55100
                     /locus_tag="JA154_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HAT7994306.1"
                     /translation="MPNLFPQSETFETVELKNNDENELDLKGSFLFDFEKGEFVKNAD
                     GTLKKCDKVQAYKQWCQKAILTPRYKKAAYTNIYGSEIKDLIASNLSQSAKELEITRL
                     IKETILVHPYTKEVSNFIFVWLENSRLVNYEFDVLTIDDENITIDGNIKR"
     gene            55109..56245
                     /locus_tag="JA154_00360"
     CDS             55109..56245
                     /locus_tag="JA154_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994307.1"
                     /translation="MERELPIPLFLTEEEDSVHERMLSNFQDVSTLEGDFIYDATRPT
                     AEQIAELKQLGLQNNLRIAFPQTSYGEYLEWLGECKGVFKNQPTKSVGMVTFNGAQGT
                     IITKGTIVTTIATDEKQSIEFELLETKTIGANETVDIKAECRIAGTIGNVSNNTITVL
                     LGSISGVKSVSNKEDFRGGTDIEDEEHFRERVLVAEQEDKLSGASSDYIRWAKEVDGV
                     GYAYVVPEWNGAGTVKVLILDKNRKAATQELIDKVQEYIYPLNISEGENRDGKAPIGA
                     LVTVVTPDTLLINVKASFIFSNGFSEETVLNNLKTKIDKYLDKIDLGGTVSYNAIQAI
                     VGSMMLTDEGIEDFSNLTINDVKENIKLQDQVVGIGEIVNEVVG"
     gene            56242..56871
                     /locus_tag="JA154_00365"
     CDS             56242..56871
                     /locus_tag="JA154_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994308.1"
                     /translation="MIASKKGKEMLLTLSPIYEQSIIMQSLYEAIGSEFDNLELLNKE
                     IELQLFPQSATWGLEFWENRVGLSTNIDEDIEARRRKVIAKLQSKYIMTPKRMAMILQ
                     SYTGANIKINENISPYTFGVELTSTQGFPKDLEDLYKRVNVIKPSHLAVSYKLVSLLK
                     SKTYFAQTAIMSEEITVYPYTSKEVKASVKAKFALAHNMSSETLTVYPR"
     gene            56884..57990
                     /locus_tag="JA154_00370"
     CDS             56884..57990
                     /locus_tag="JA154_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAT7994309.1"
                     /translation="MADEQFYTILTNIGKAKIANAGMLGKSVILEKIQAGDGGGNYYN
                     PTEDQTALKNKVWEGNINAFDNDENNPNWIIATACIPGSIGGFTVREMGLIDNEGDMI
                     AVCKSPETYKPKVDNGAMKDLYLKFIIEVSNVEKVTLVVDPTAIFLTKKDEEKILTNI
                     NKLDTKIDTTKTELTSNIETTKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQIITI
                     DDWVEDAENGFKATVTHSLLTQRIVVNIIDATTKENAVTNFKIIDDNSIEVRSETRSE
                     LNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDSISSIQLIDTSISITDANDRF
                     TSDKLDGVLEEIMVEISGQRTKGITIVNNLIDMI"
     gene            58004..58642
                     /locus_tag="JA154_00375"
     CDS             58004..58642
                     /locus_tag="JA154_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994310.1"
                     /translation="MTEKLTENASLGELMAALENVQTDFQTGKNNISSALGSPFIGTD
                     KFGTTKTKIETLKNVLVETINSKNVSATSSETFTNLIEKVNWIFQSIEIFSLKNRIQA
                     TTLNTPSIVYNEVSSIKGTLRFTGELKASKMRADYATAKIEILCGNRKEYFYVTDDTP
                     SASSSFVRFTKDIIVENGMDIKVQILLTSVGAGNLDGSYAARAEIEKLTILR"
     gene            58652..58864
                     /locus_tag="JA154_00380"
     CDS             58652..58864
                     /locus_tag="JA154_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994311.1"
                     /translation="MKKQVYYNSLDEKEKIISENSNLYVIEIYETLNENYLVLSSSPI
                     QDEKLSYEELENELLIMTNELQGGLL"
     gene            58864..59046
                     /locus_tag="JA154_00385"
     CDS             58864..59046
                     /locus_tag="JA154_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994312.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGIEPTA"
     gene            59080..60315
                     /locus_tag="JA154_00390"
     CDS             59080..60315
                     /locus_tag="JA154_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994313.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENNFTSDNVEGALKEVFQN
                     GVNAKNNVVTALNSKGAEVTTSDTWEEIKNKIDIKEGRLDLRETILANSYSSYLVTNG
                     AIKYIEKYSGSLKALEYEEPYFYAIKGTHLIKINAIDESVIFDITLANANFSCICVTQ
                     EYLFISDNTKLYKINKLTGNEAQSIEGSYYKLCTYGEFIYGIYGDETSSILHKIRISD
                     MYIMLTKDMSSDRIYDFERGKFVCNNNGIYATTEHSNSSGLTECYLTKINFDFSVAKS
                     FRIGGYLYEKNIKFLNDFVFVSDSSRSIEVESGKKSGLAKYDANLNLITYGGSDRYEN
                     FEIYNGYIYTFNSLSSSPFIKINLNTLKEVNSYRTLINTNPQKGMFIINNIIFFIGGG
                     IHRNILSKKVYSDEKGEEL"
     gene            60312..60611
                     /locus_tag="JA154_00395"
     CDS             60312..60611
                     /locus_tag="JA154_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994314.1"
                     /translation="MIYLGNFMETDEQNIKYIGMIHYKPTLLSEEELKNGILIEKLPV
                     QQYVENKEAKLFINIDTKEVFYRYTDIKSSIEDKVNSTEQTIADLTFQLMSNGVI"
     gene            60612..60749
                     /locus_tag="JA154_00400"
     CDS             60612..60749
                     /locus_tag="JA154_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAT7994315.1"
                     /translation="MNWYKIITDFYNNGNWTKEQVKTAVEKNKITATEYKEIVGEDYI
                     A"
     gene            60877..62442
                     /locus_tag="JA154_00405"
     CDS             60877..62442
                     /locus_tag="JA154_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994316.1"
                     /translation="MATVYEFNYTGSEQSVTLKPGKYKFECWGARGGALGTPFESGFY
                     YGYGGYCSGEITLKKETTLYLYVGIDGRKGYNFNSAGYGNGASGGGATDIRLIGGTWD
                     NEQGLLSRIIVAGGGGGTYDKQHGGDGGGLKGTLGTSSTGAAAHGGTQFEGGRGRDKD
                     GSCDGFFGKGATPKNPSSQSGGGGGWFGGAYPASGFGNGSGGGSGYVLTKDSYKPPGY
                     TPTSEYYFDNVVMTTGGNTTVVGNYSDGRAKITLLQALPFLNISSYNSTTATFKADHT
                     DPTLLTKIEIFIDDILKETITTDLILEKTINYTLEDNALHTLKIVVTDSTNATAEKIV
                     SLSKNIMPLGDDASLQDIASKVSEIKEGLINGKTSIINVLALKNIESSLNNSLVELSE
                     KVKESFDSSDASLQDLINQLTQANNTISQLNSKYKYSSGTFTYYTGAYPNGLNIGFRP
                     DIISYTVINAVEEKYYCVYTPLFYLFYNYTTGEKYLDVNHNHYGITFTDNGWIPKGPV
                     NNGVYSYIAVKCC"
     gene            62500..62730
                     /locus_tag="JA154_00410"
     CDS             62500..62730
                     /locus_tag="JA154_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994317.1"
                     /translation="MNEELFKENLKRHEVTINKHNDEIDELKVANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQKGIF"
     gene            62750..63007
                     /locus_tag="JA154_00415"
     CDS             62750..63007
                     /locus_tag="JA154_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAT7994318.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLSPVAVLQGVICWGISIGINQTYKQLKEENK"
     gene            63007..63819
                     /locus_tag="JA154_00420"
     CDS             63007..63819
                     /locus_tag="JA154_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT7994319.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPRVNAGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICKKLGTIFKNRGAKLDKGLYILNSSKPTAILIESFFCDNKDDYEKAKKF
                     GYEGMAKLIVEGVLNKNIGNDGVKLMYKHTIVYDGEVDKIPATVVGWGYNDGKILICD
                     IKDYIPGQTENLYIVGGGACNKIGSITKEKYTMIKGNDRFDTLYKALDFIDR"
     gene            64069..64560
                     /locus_tag="JA154_00425"
     CDS             64069..64560
                     /locus_tag="JA154_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994320.1"
                     /translation="MAYENGTDFIDYFPDCIEKYKEDNKIFEKAILLLNLYMQKPTWP
                     HCVNEYKSQAEKILGEDPEFIGKYGIQLRKFYDEDENAKVSKEFKNDLYNFSKSTYDI
                     LDNVYFSKSHPFNLSSIYSTSRDDDFIIKIFRYDNNFLELEMSKREVESLINYLSDLL
                     KEE"
     gene            64562..65128
                     /locus_tag="JA154_00430"
     CDS             64562..65128
                     /locus_tag="JA154_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994321.1"
                     /translation="MLGAKKEKEFNLLSFNEKKIEKHKCKNYHNDSSKDKYKYSQSEF
                     IAATLKEADDMNEEERRILEAEKRILETEKRVHQDNKEVKESIDKQFNDIKGIFKEYK
                     NDLDKDRIELKNDLARERAELKSDLKEAINEHKKVTEKDVSEIKNSMKGIEDRIDSTN
                     KWIIGLCITTIIGIATMAITIGISIWPK"
BASE COUNT        26759 a         6913 c        12353 g        19596 t
ORIGIN      
        1 agtaagtagt ttaagcattc ttcttatatt aatctttgag aataatgtat ttaaaaaagt
       61 tagtaaaaaa tttaactatt gtatattaat gatagttgct attgatatat tatctaaaaa
      121 aattaagtat gaaaagatta ctgctaggac aatcttttca tacttaattt ttaatattta
      181 tattatccaa aattttcatc tttttaactt agaatttgag aatcactatt aggaaatagg
      241 ctttgattta tgtagggtta aaaatttcac agaatatcta actttatact aaatccatca
      301 aaaaatatac gccatacaaa agcgcataaa aaaacacata aaacaatatt tatgcgcact
      361 ttaacctcga atggtacaaa attctttact tttacagacc catattatat aattaagtaa
      421 ataatgtata tttaggtgac atattggata agctaattta tttattttat taatataata
      481 atactataaa agttatataa agtggtttac aaaaaagtta cagatagagc ttttattaat
      541 gtattgtgta ttagtatttt atccttaggt acacttcacc tacaaaatag aatcataaga
      601 taattatggg aaaattttag gaggtttatc gtgaagaaaa agatattaat accagtattt
      661 gcatcagtta tggcattatc agtaagttca attgtaaatg cagatgaagt aaatgattca
      721 tctcaaaata aggatgataa aacaaatact gaattaaatt taggtgaata taaagaagta
      781 aaatataaag ttgcaaaaat aaaagatgga gtagctataa agataagaga agaaggtcaa
      841 gttcaaaaca tagcttattc tggagatgaa tttacggttt taggaactca aggagaatgg
      901 gttaaggtta aagttgaaga tggcgaaggt tggttagcta ctagatatgt agatatatct
      961 gaaggtgtag gatatactaa cgctgataaa gtaaacttaa gaaaagataa gagtgaaagt
     1021 tcagaagtaa ttgaagagct ggaaaaagga agttctttat tggttttgga agataatgga
     1081 gattggttaa aagttaaaga tggggaaaca gaaggatatg taaaatcatc ttatatatct
     1141 gataaagctc ctgtaataga agagccacaa gtaaacccaa gtattgacca aaatgtagat
     1201 tctacaaata ataatcaaca aaacaatgca aatactaatc aacaaaataa taattcaaat
     1261 gtgccaacag ctaactctaa tgcagttcaa gctgtactta acttggcata ttctaagcaa
     1321 ggttgtccat atgtttgggg agcagaagga ccaaacactt ttgactgttc tggatttaca
     1381 caatatgtgt atagaaatgc agtaggaaag aatataccaa gaacatctaa agcacaatct
     1441 aaatatggac aaactgtaag taaagcaaat ttacaaccag gagatttagt attcttcaca
     1501 actaatggtt ctggttctgt tagtcatgtt ggaatatatg taggtggggg taatatgata
     1561 cactcaccat caacaggaaa aacagttagt gtaactagta taaattcatc atactataca
     1621 gcaagatttg ttacagcaaa aagaatatta taatcggttt gtaatatgac aaattgataa
     1681 atacataaat atatataaac gctctatatt atatttaatt taatcatgga aacaaaataa
     1741 tgattatatt ttaaatgtac tatagagcgt ttttgtatca ctttaaatct aatcaagatt
     1801 caagttaata tgactgttaa ataggagcct tttcatatga tgattatttg tgcaatccct
     1861 tgacaggata gggtaaaggt ggtaagctac taacagtaca aaaatacttt taattttagt
     1921 tcagagaggc taaaaaagga ggacgaaaga tgttaaaatc aagaaattta atccaaccag
     1981 aagacttttc aatagaagaa atagatgaaa tattagaatt agctcaaaag ataattgaca
     2041 atccgtcaaa atattctcgt atatgtgaag gaaaactatt agctacatta ttttatgaac
     2101 catcaacaag aacaagatta agctttgaat ctgctatgaa cagattagga ggacgtgtag
     2161 taggcttttc agagccaaat tcatcctctg catcaaaagg tgaaactcta ggagatacaa
     2221 tgaggatagt atctggttat gttgatataa tagctatgag acatcctcaa tcaggtgcag
     2281 cttcagaagc agctagatat acggaagtgc catttataaa tgcaggagat ggcaaaaacc
     2341 agcatccaac tcaaacactt acagacttac ttactataaa atcattaaaa ggaactttag
     2401 aaagccatac aataggatta tgtggtgatt taaaatatgg aagaactgtg cattctttgg
     2461 ttaaggctat ggcaagatat aaaaatacaa agtttgtatt tatagcacct gaagagttaa
     2521 aaatgccaga ttatataaaa gaagctataa agggacatgc atattatgaa actaataatt
     2581 tggatgatgt tataggaagc ttggatgttt tatatatgac tagagttcaa caagaaagat
     2641 ttgaagataa atcagagtat gaaagactta aaaactatta tattttaaat aaagctaaat
     2701 tagaaaaggc gtctaaagat atgttagtaa tgcatccatt accaagagta aatgagatag
     2761 acatagatgt agatagtgat gatagagctg tatattttaa acaagctaaa tatggaatgt
     2821 atgttagaat ggctttaata ataaaacttt taggtataaa tgaagactaa tagttatcat
     2881 ataatagtaa aacaaaggag attttgggat gtataaaatt ctggaaaata tatatatagg
     2941 cgaagatatg tatagaatga aagtaaaagg taattttgaa ggtaaaatgg gacaattcta
     3001 tatgttaaga gcatgggata cttatccagt actttcaaga cctataagta tacatgatat
     3061 agatgaagaa gggataactt ttctttataa agtagtagga gaaggaactc aaattttgag
     3121 taacctaaag gtaaatgata ctataaaatt ggaaggccct tatggtaatg gatatgctaa
     3181 ggttgatgga aaggtagctc tagtaggagg aggaatcggt gtagctccac tatatttggt
     3241 tgctaaaaat ataaaaaatt gtgatgcata tcttggcttt agagaagatg ttatattaga
     3301 agatgaatat aaacaagtat gtaataaagt atatacaaca gtaggaaata cttttgtaac
     3361 agatataatt gatgttgaga aatatgatta tatattaaca tgtggtccaa ctccaatgat
     3421 ggaaaaatta gttaagatgg tagaaggtac taaaacgaga attatggtat ctcttgaaaa
     3481 ccatatggct tgtggagtag gtgcctgttt agtatgtacg tgtaaaacta atggaggaaa
     3541 taaaaagact tgtaaagatg gtccagtgtt ctggggagag gacgtgattt ttaatgggta
     3601 atttaagtgt gaaatttgga agtgtagaat ttaaaaatcc agtaataatg gcgtctggaa
     3661 cttttggatt tggaaaagaa tataatgaga tttatgatat acaaaaactt ggtggtataa
     3721 gtagcaaggg tcttacttta aataaaaaac ctggtaacaa tggtatgaga gttcatgaga
     3781 caccatccgg tatgatgaat agtgttgggc ttgaaaaccc tggagttcaa ggatttatag
     3841 actatgaatt accttttttt agtaaactag acttagtcag aatagctaat gttggaggtg
     3901 ggacattaga agactactta ttaggggttc aaatgttaaa tgacaagcct atagacatta
     3961 ttgaattaaa tatatcttgc ccaaatgtca aagctggtgg aatggctttt ggtataaaaa
     4021 atgaagtggc aagagaagtt gttcgtgagg taagaaacat aacgaaactg cctttagtaa
     4081 taaaattatc acccaatgca gaagatattg taggaatggc aaaagtttgt gaagaagaag
     4141 gtgctgatgg agtttcatta gttaatactt ttaaagcaat ggcaattgat ataaaaaata
     4201 gaagaccagt atttgaaaat gtatatgctg ggttatctgg accagctata aaacctatag
     4261 ctttgagaat ggtacatgaa gtgtgtaaaa atgtaaatat acctgtaatg ggaatgggcg
     4321 gaataactaa agccacagat gctatagagt ttataatggc aggagctact tgtatccaag
     4381 ttggaactgc aaattttatg aatcctagaa taggtattga aataatagat ggaattaatg
     4441 aatttatgga tagagaagga ataaaaagtt tagacgaaat aagaggaatt atataaggta
     4501 gaataaatag tattttaaag ataaaaaata tagattaaat aaacaataat tttataacat
     4561 taacatggag gaaaaataat atgagtaaag tagatgtagt ggatatatta aagaaaagtg
     4621 atgcattgtt ggagggacat tttttattat cttcaggaaa acacagtaat agatacgtac
     4681 aatgtgcaaa agtattaaga tttccagaat atgctgctga ggtattaagt acagttgttg
     4741 aacaaataaa agacttagat atagacttag tagtaggacc agctatgggt ggagtaatag
     4801 tttcttatga gttaggaaga caattaggaa aagaagctgt atttactgag agaaaagaca
     4861 atacaatgga gttaagaaga ggatttgaag ttaaaaaagg agcaaagata ataattgctg
     4921 aagacgttgt aactactggt aaatcaacta tggagacaaa aagagtatta gaagccttag
     4981 gtggagaagt tgtaggtgtt gcatgtatag cagatagaac taatcatgat ataggtatgc
     5041 ctatatatag tgctataaaa cttgatattc aagtttatga atctgatgag tgtcctttat
     5101 gtaaggaagg aaaattacca gttgttaagc ctggaagtag ggagttcaag gaattaggga
     5161 tgtaataaaa acttaattat ttatagtgtt acttaaaaaa tgtaaatttt ttagagtaaa
     5221 aatttgtaat ataacatata acttaaaaat aaaaagcagt tagccttata aatgaaggtt
     5281 agctgttttt tttgttaaaa atatatttac aaaataagag atagtagcag tacaatataa
     5341 gtataaggta aaagtattat atattaggga ggggtaagta tgaaaaatca attaaatgaa
     5401 aatcataaaa aggttgtatt ttttggagta ggtgctgtag gagctacttt tgcagaacaa
     5461 ttttttaact ctaaatatga ttttaaaatt ctttgtgata atgaaagaaa gaaaagatat
     5521 ttagaagaag gatttataat aaatggaaaa aggtatgatt ttgattatgt aactaaagat
     5581 gagtataaac aagaggctga ttttataatt ataggtctaa aatataataa tttaaaagaa
     5641 aatataaaag aattagatgg attagttgga aaaaatacag ttataatgtc tctgctaaat
     5701 ggagttgata gcgaagagat aataggagaa agatttggaa ttgaaaaaat ggtatattca
     5761 tatgttacca atatagatgc aaagaaaatc aataataata ttatacacac tactaatggg
     5821 ataattgtat ttggtaacaa agataatagt gaagatagaa aaactaatat aataaccgaa
     5881 gtctttgatg atgtaaatat agaatatact ttatcaaaag atattcaacg agatatgtgg
     5941 tggaagtaca tggttaatat tggtgtaaat caaacttcag ctatacttgg tgcaccttat
     6001 ggagtttttc agagttctga gcatttaaga gaattagcaa aatctgcaat gagggaagtt
     6061 gttgctatag cacaagcaaa agacatatct cttacagaag atgatgtgga acattcatta
     6121 catagaatac tagaacattc aaaagaagga agaacatcaa tgcttcaaga tgtggaagct
     6181 catagactta cagaagtaga tatgttttct aagaatatct gcaaacttgg aaaaaaatat
     6241 aacataccta ctcctataaa tcagactttc ttttatatga taaaagtaat tgaaagtaga
     6301 ttttaaaggg tctttgtaag aaatattgat aattattgat tttaaatttt attaatgtta
     6361 tatactattt aagttactaa atatttttaa ctcttcaatg aaaatagttg ctgcttttga
     6421 taaagttata tcttttggaa atatatatga gaaatgtctg ttgtagctgc ttccaagctc
     6481 tatgacagac agctcattgt ttagaacagg taagcttgtc actagattag agactattgt
     6541 aattcctaaa ttatttctaa cagattcttt gacagcataa ttacttccaa aaaccatcat
     6601 actttttgga ataatttctt taacacttaa aaacatatct aaataatctc tggttccaga
     6661 accatcctcc ctgactatcc atttttggtt ttgaagttta tcaaaactaa aatcttttaa
     6721 taaatgactc ttgtatggaa gtgctaaaac catcttatct tcaaaaaaat attcttgaat
     6781 aaatgaaggg gaagagcaag tgccttcaat aagaccaatg tctaaaatat aatccttaac
     6841 atgagaagat acgattgatg tattttttat aaaaacttct acatctatgt caggatactt
     6901 tttagaaaat aatgctagaa aatttggtaa aatatactct ccaatagtta aacttgcacc
     6961 aatctttaag tgaccagtta tagcatctga aacttggctt acatcatgat aagtaatatt
     7021 taaaagattt aatatttctt tagctctttt gtacaagatt tgtccacttt ctgttataaa
     7081 aatggttttt tgtttaatag acctgtttat aagcacaact ttaaaataat tttctagatt
     7141 ttttatgtgt ttacttacac ttggttgtga tagatttaaa tattccccag ctttagtaaa
     7201 gtttttgtat tcaacaacag ctacaaatgt ttttaattct tcaaacaatt ttttgtctcc
     7261 tttacctaat gaaattttac attttttata tactttctac agataattat actatatata
     7321 ataaccatta aaaacattaa tcattacgat attatatatt tattttactt atggttatta
     7381 caattatata ctattgctat gaagaaaact atattaatta taaggtaggt atattatgtt
     7441 aaagaaaaaa agcagtttga cattaaaaac tataagggaa atattaccag gactgtttgt
     7501 atcagtatta gtaggttata ttagtatatt tatttctatt ttaattccta aggtaggggc
     7561 agcatcaatt tctatatttt taggtatgtt tgtaggaaac ctatttttaa atcaaaaggt
     7621 atttcaaaaa ggttataaat tttcagagac agatttgtta tcatattcaa tagtactatt
     7681 aggagcaaca ctcagtgtat ctaccttaat agacttaaaa gtttcaggaa ttttatttat
     7741 aatattgcaa atgacaataa caataatagc tgcactttat ataggtaaaa aactaggatt
     7801 tgaagaaaac tttagatttc taatggcaag tggaaatgca gtatgtggtt cttctgcaat
     7861 aggagcaaca gttccagtgg ttaatgcgac agataaagaa aagggaattg cagtaactat
     7921 tgtaaatgtt actggtatat ttcttatgtt tttacttcca ataatatctc aatttttata
     7981 tagccatgaa cttgtaaaaa cttcagcaat gataggtgga actttgcaat caattggtca
     8041 ggttgtagca agtggagcaa ttgttggaga aaatgtaaaa gatttagcta ctatttttaa
     8101 gatagtaaga gtaatatttc tagttgttgt tgtattagta tttggacata tgaaaaatca
     8161 atctaatagg gaaattcttg aagaagaaaa ggatgagata aaaaagaaaa aagtgactat
     8221 accatggtat gtaattggat tctttataac ttgtgcactt ttttcaatga acattatacc
     8281 acaagaagta tctgtgttgt gcaaagaaat aagtaacaaa ctagaaataa tagctttagc
     8341 agcaattgga ttaaaggtta atgtaaaaga tttagtgaaa caagggaaag aagtttcttt
     8401 atatggttta tttgtaggta tagtacaagt tgtatcagct gtagttttaa taaaaatatt
     8461 tatatagcat aaaaatagta tttgtaatat taatttgatt aattaaaata atatgatact
     8521 aataatttat aatataaata aaaaatgaat ttcgataagt ttaagtgggt ataaacttat
     8581 agaaattcat tttttattta aggtaaaaat ttaaagtaaa atattatttt acataataat
     8641 aaccttataa attttgaaat tacttatctt tgtgttaaaa taagtaaaat gcacatatta
     8701 gagctaagtt tttaagtaaa taaacaaaat gaaaatataa atattaagga gatttggtat
     8761 atgaatgttt atgaaaaagg taatggagtt atactagaag gagtaacaga ttttgaccca
     8821 gtacatatat ttgagtgtgg tcaatgtttt agatggcaca aacaagaaga tggctcatac
     8881 actggagttg caaaggggag aatattaaat gtcaaaaaag agaatgataa aatatattta
     8941 aataatacta atttaaagga gtttaacagc atttggtata attactttga tttaggaact
     9001 gattatacag aaataaagaa taagctaaaa aatatggatg aatatttaaa taaggctact
     9061 gaatttggat ggggaataag aatattaaga caagatggat gggaaatgct tatatcattt
     9121 ataatttcct ctaataatag gattcctatg atacaaagag ctatagaaaa tttatcaaga
     9181 aaatttggta agtatatagg tgaatatgaa ggtaatgaat actatgcatt cccaacacca
     9241 gaagaactaa ataaggcctc acaagaggaa ataagagcat gtcaaacagg ttttagagat
     9301 aagtacataa aaagtactac acaggcagtt atagagaata atgatgaggt atctgaatat
     9361 acaaatttaa gtactgagga ttgtagaaaa gagcttttaa aatttaatgg agtaggacca
     9421 aaagtatgtg attgtattgc attatttggt atgcaaaaat atgattcttt tccagttgat
     9481 gtttgggtta agagagttat gcaagaattt tatatagatg aagacatgag tttacctaaa
     9541 atgagaactt atggaatcga taaatttaaa gaaatgtctg gatttgctca acagtactta
     9601 ttttactatg caagagaact cggtatagga aaataaatta agggggagaa attatgggag
     9661 taataggaac aatatttctg ttctatttaa ttatatcata tctagctgga gccataattt
     9721 ctgtgattat attgcttgaa aatagagacc ctgcaaaaac aatgtcttgg ctattgatgt
     9781 ttattatatt tccaggtgta ggtttaatga tatatgctat ttcaggtaga aatattagaa
     9841 aaagaaaatt atttaagact caaaaactag ctaataatat aaaagaaaag aaactatttg
     9901 atacattaga aaagataaca gaaatagttg aattagaaaa agaatctata aaacaaaata
     9961 agcttttaag agatgaagag gatggtagtt atagaaaaag ggtaataaat atgttgttaa
    10021 agactggtat gtttcccttt actaaaaata ataaagtgga tgtatttgta gatggaaatg
    10081 aaaagtttaa aagacttata gaagatataa gagaagccaa agaccatatt catttagagt
    10141 actttatcat caaagatagt gaaataggta gggtgttaaa agaagaactt ataaaaaagg
    10201 ctaaagaagg cattaagata agaattttat atgatgatgt aggatgctgg agattttggt
    10261 ttaatagaaa atttttccga gaaatgaggg aagtaggtat agaaattgca gcatttttac
    10321 ctacaaaatt tcctataata ggagggaaat taaactatag aaatcacaga aaaattgttg
    10381 ttattgatgg aataattgga tatacaggtg gaataaatat aggagatgaa tatttaggca
    10441 aaaatgataa gtttggatat tggagagata ctcatattag gattaagggt atttctgtat
    10501 acatgcttca aatgacattt ctgattgatt ggtattatac gacaaaagaa gtattagtga
    10561 ctaaaaatta tttcccaagt gtagggaatg ttggtgaaag tatgatacaa gttgttgcga
    10621 gtggtccaga cagtgactgg gaagatattc attatgctta tttttctgca atatgtcagg
    10681 ctagaaaaaa tgtttatata gaaactccat attttattcc agatgaaagt ctactaaaag
    10741 ctataaagag tgctgctctt agtggagttg atgtgagaat aatatttcct aaaattgcag
    10801 accacaagat agtaaatata gcatcttatt catattttga agaaatatta agagcaggtg
    10861 gtaaagttta tttatataat aaaggattta tacattctaa agtcgtaata atagatgata
    10921 aaattgcttc tgcaggtaca gctaatatgg atttaagaag ttttatgctt aactttgagg
    10981 taaatgcatt tatctatgat gaagaagtta ttagggtaat gacagatgat ttctttgagg
    11041 atttaagtca ctgtgaagaa cttaatttgg aggtatttaa gaatagaaat atcatacaaa
    11101 aaatcaaaga atctgtagca agattatttt caccaatatt ataaaaaatt attaaaaaag
    11161 gtattgaaaa ttatgtgtga ttggtatatt ataataattg ttagcactct attagataga
    11221 gtgataataa ataaaaagta tatatataaa tctacatata taagtagtat taatttaaaa
    11281 ataactaaaa acataggcga actagtctat acttaatatt ttaggaggcg tgaataatga
    11341 aaataagacc attagctgac agagtagtaa ttaaaaaagt agaagcagaa gaaaaaactg
    11401 caagtggaat agttttacca ggagcagcta aagagcaacc tcaaatagct gaagttgtag
    11461 aagttggacc aggtggaata gttgaaggga aagaaataaa aatggaattg acagtgggag
    11521 ataaggttat attccaaaaa tattctggaa cagaagttaa gatagaagga caagaataca
    11581 caatactaag acagagtgat gtattagctg ttattgaata aatatagaat aaatttatta
    11641 ggaggggttt aaaatggcta aagaaattaa attttcagag gaaacaagaa gagctttaga
    11701 agctggtgta aataaattag cagatacagt aaaagtaaca ttaggaccta aaggaagaaa
    11761 tgttatatta gataaaaaat ttggttctcc acttataact aatgatggag taactatagc
    11821 aaaagagata gaactagaag atagatttga aaatatgggt gctcaattag ttaaagaagt
    11881 agcaactaaa actaatgatg tagctggaga tggtactaca actgctacag ttttagcaca
    11941 agctataata agagaaggtt taaagaatgt aacagcaggg gctaacccaa tacttttaag
    12001 aaaaggaata caaaaagcag taacagtagc agtagaagaa ttaaaaaatc aatcaagaat
    12061 agtagaaaca caagaggcta tatctcaagt tgcttctata tctgctggtg atgaagaagt
    12121 tggaaaatta atagcagaag ctatggaaat agtaggtaaa gatggagtta taactgttga
    12181 agagtctcaa actatgaata ctgaattaga tgctgttgaa ggtatgcagt ttgatagagg
    12241 atttgtttct gcatatatgg ttacagatgt agataaaatg gaagcagttt taaatgaccc
    12301 atatatatta attactgata aaaaaatatc taacatacaa gaattattac cagttcttga
    12361 acaaatagtt caacaaggta aaaagttatt gataatagct gaagatgtag aaggtgaagc
    12421 attatctaca ttagtagtaa ataaattaag aggaacattt gatgtagtag cagttaaagc
    12481 tccaggcttt ggagatagaa gaaaagaaat gcttcaagat atagcaatac ttacaggtgc
    12541 tcaagtaata tcagaagaat taggttatga tttaaaagaa gctgatttat ctatgctagg
    12601 tagagcttca tctgttaagg taactaaaga aagtactaca atagtagatg gttctggaga
    12661 taaaaaagct atagaagata gagtaactca aattaaacat caagtagagc aaactacttc
    12721 agattttgat agagagaaat taatggaaag attagctaaa cttgctggag gagtagctgt
    12781 tgtaaaagtt ggagctgcta cagaagtaga gttaaaagaa agaaagttaa gaatagaaga
    12841 tgctcttaat gcaactagag cagctgtaga agaaggaata gtagctggtg gaggaactgc
    12901 ttttgttagt gttataccag caataggaac actaattgag agtttagaag gagaagttaa
    12961 actaggtgct caaatagtta aaaaagcgtt agaagagcca ttaagacaaa tagctataaa
    13021 tgctggtctt gaaggtgctg taattgtgca aaatgttgtt aattctgaag cagaaactgg
    13081 atttgacgca ttgaatgaaa aatatgtaaa tatgatagaa gctggtatag ttgacccaac
    13141 taaagttagt agaagtgctt tacaaaatgc tgcatcaata gcaagtactt tcttaactac
    13201 tgaagctgct gttgctgacc ttcctgaaaa agaagatgta ggaatgccag gaatgggtgg
    13261 cggaatgcct ggtatgatgt aatttaaaaa atttaaaata taaattttaa tataaagaac
    13321 tatttcgact tagattttaa actctaagtt gaaatagttc ttttgtatat atctaatttt
    13381 tgtatatcta taaattcaat acacatattt agataatcag tatctagttt gtaataatat
    13441 tttagagtag tattgttatt ttttaggtta tgattttaga tatatattaa ttaaggtata
    13501 attgttgata gagaataaat caaatttaaa aggatgcgat aatatggact taagaaaatt
    13561 gcttgaagaa gttaaaaatg aaaatattga tatagatttt gcgttagaaa agttaaaaga
    13621 tttaccttat gaagaccttg gatatgcaaa tatagaccat catagagaat taagaaatgg
    13681 gtatccggaa gttatttatt gtgaaggtaa gagtgatgag catattttag ggattattga
    13741 taaaatgaat caaaaaagtt ctaacatatt ggggacaagg tgcagaaaag aaacttttct
    13801 gaaaattaaa gaaatctata atcatgctga gtacgaagag gcgtctaaaa ttcttaagat
    13861 tcaaaatcat gatatagaga atataggaaa aggtaaaatt gtgattgcta cagggggaac
    13921 ttctgatata ccagtagcag acgaagctta tcatacagct aagtttttag gtaatgatgt
    13981 tgatagaata tatgatgttg gagtggcagg tatacatagg cttttaaata aaagacataa
    14041 aatagatagt gctagagtaa tagtagccgt tgcaggtatg gaaggtgctc ttgcaagtgt
    14101 aattggtgga ctggttgatg ttccagtaat agctgttcca acttctgttg gatatggagc
    14161 aaattttgga ggattagcag cattactagc tatgttaaat agctgtgcat ctggaatatc
    14221 tgttgtaaat attgataatg gatttggagc aggatattta gcatcaatga taaataaact
    14281 gtaaatacat tgatataaat taaacaaaac tatataataa aatttaattt ctattatatt
    14341 tttactatta aaattgagga gacatatatg agtgaaaaaa tactttattt tgatataata
    14401 agcggtatat caggggatat gacccttgca agtttattaa atttaggtgt gcctaaggaa
    14461 atttttttgg aggaaattca taaactaaga atgagcgatg aatttaatat aaacatcagc
    14521 tcgaaaactg aaaatggaat agtaggtaca aaagtagaag taattacaaa agaaaaacat
    14581 acacatagaa atttagtgga tatatttgag gttatagata aaagcactct aaacgaaaat
    14641 gtaaaatcta aagcaaaaaa aatatttatg gtaattggag aagcagaagc aaaagttcat
    14701 ggaactacaa tagacaagat acattttcat gaggtagggg ctatagattc aattgtagat
    14761 atagtaggag cttctatatt agttgactta ctttgtgtag ataaagtttg tgctagtaca
    14821 gtgtctgttg gttcaggatt tgtaaaatgt gaacatggaa ttatacctat acctgcacct
    14881 gcaactatag aaattttaaa aggtgtgcca attaaattaa ataatgtaaa tggagaatgt
    14941 acaactccaa caggagctgc aatcataaaa gtcttgtgtg atgagtttgt agatgagctt
    15001 gaattttctc caaagcaaat tggatatggt ataggtcata agaagtttga agtaccaaat
    15061 atactcagaa ctgtattagg tgaaaaaaaa aagaagaatt aatttatgaa attagtgcaa
    15121 acattgatga tatgtcttca gaaatctatt cttatttgta tgaaaaaata ttagatgaga
    15181 aagcattaga tatttataca gagagcattt atatgaagaa gaatagacca gcaacaaaag
    15241 tatcaatttt atgtaatgaa tcagatttag aaaaatttat taaaatatta cttttagaaa
    15301 ctagtacttt tggagtgaga tataatgcat ataatagaga aattctagag agaaaatttg
    15361 taaaattaga tacaaaatat ggagtggtta cagtaaaact agggtattat aaaggaaaac
    15421 tagtcaaggc tactccagaa tataatgaat gtaaaactat agcaaaaaat gaagatattc
    15481 ctataaaaaa agtgtttgct gatataaatt ttagaataaa agaagaattt agtgaaaact
    15541 tattgacata agtaataaat tttgataaat taatattcag ccttattaaa taaaagtaaa
    15601 cagaaaaaaa tatataaaat ataaactcac tcgtatatgc tcagaaatat ggtctgagag
    15661 tctctaccaa gataccgtaa atatcttgac tatgagtgaa attattatac caaaagacct
    15721 agttaagtaa atgtgtaggc tgagttaaac tccctatgtg ttggctataa gataaaggta
    15781 ttttggaagc ctgtatagta atttcacttt tgctagaaat tatggaagtt actatacagg
    15841 ttttttgtgt aaaaagattt aatataattt agactgtatt gtgttaaaat aaatatataa
    15901 aaactatatt atacaaggag agatgtaaat ttatgaaaca tgaattagta cttgtaatag
    15961 attttggagg acaatataat cagttgatag ctagaagagt tagagaaaac aacgtatatt
    16021 gtgaaatact tccatgtact gcaagcatag aaagaataaa agaaaaaaat cctaaaggga
    16081 taatatttac aggcggtcca aatagtgcgt atttagaaga ttctccaaca atatcaaaag
    16141 aaatatttga actaggagta ccaatacttg gaatatgcta tggtattcaa ataatgtctc
    16201 atgtattggg tggagttgta agaaaaggta ataaacaaga aaaagaatat ggaaaaacag
    16261 ctataactta tggaaaatca tcattatttg aaggaataac tacaaatagt gtatggatga
    16321 gtcatacaga tttaatagaa aaagttccag aaggatttac tatagttgca aatactaatg
    16381 attgtccagt agcagctatg gaaaatgtag aacgaaacct atatggagtt caattccatc
    16441 cagaggtaga acactgtttg gaaggggaca agatacttac aaacttctta tataatattt
    16501 gtaaagtaaa aggtgactgg actacagatt cattcataga agataaaata aaagaattaa
    16561 aagaaaaaat aggcgataaa aaagctttat gtgctttaag tggaggagtt gattcttctg
    16621 ttgctgcagt tcttattcat aaagcaattg gggacaactt aacttgtata tttgttgacc
    16681 atggtttact tagaaaaaat gaaggaaatg atgtagaaag aatatttaga gagaaatttg
    16741 atataaattt aataagagtt aatgcagaag atagattctt atctaaatta aaaggagttt
    16801 ctgaaccaga agctaagaga aaaataatag gtgaagaatt tataagagta tttgaagaag
    16861 aatcaaataa attaggaaaa atggatttct tagtacaagg aacaatctat ccagatgtta
    16921 tagaaagtgg acatggaaat gctgctacta taaagtctca tcataatgtt ggtggaatcc
    16981 cagaagatgt agacttccaa gaaattgtag aaccattaag agagttattt aaggatgaag
    17041 ttagaaaaat aggattagag cttggtatag aagaaggatt aatatttaga catcctttcc
    17101 caggaccagg tctaggaata agagttatag gtgatgtaac taaagaaaaa tgtgatatat
    17161 taagagaagc tgatgctgtt tatatggacg agcttagaaa agcaggttta tacagagaaa
    17221 tatggcaagc ttttgcaaca ttaccagatg ttaagactgt tggagttatg ggtgatgaaa
    17281 gaacatatgc ttatttagtt ggtttaagag cagttacttc ttctgatgga atgacttctg
    17341 actggtataa gatgccttat gatgtattag aaagaatttc taatagaata ataaatgaag
    17401 ttgatggagt aaatagagta gtttatgata taacttctaa acctcctgga actattgaat
    17461 gggaatagta aataaaaaag tcgtaagcct tgtattttca aggtttgcga cttttaaaaa
    17521 aatctttgtg atactaaaat gatactatat atttaaatta tctagttttt gggcaacttc
    17581 aagttgctta tttgggtata aatgtgaata tgtattccaa gttgtttcta ctttttcatg
    17641 ccccaatctt tctgctatag ttaatatatt tacatccata tttaccaata atgacgcatg
    17701 agaatgtctc aaatcatgca ctctaattct ttttacatta gataacttac agcatctatc
    17761 tagctcttta gaaagataac ttttagcaaa tttaaatatt ctttcattat cttttaagtc
    17821 atatagctta gatagataat cttttatatt attatataag aaatgtggga tagggacaac
    17881 acgtttactt ttaggagttt taggagaaga aacaatatcc tctccattta gtcttatgta
    17941 gcttttttcg acgcttattt tattttcgaa aatatctttt ggagtaagag ctaacaactc
    18001 ccctagtcta agacctgtcc aaaataaaat ttcaaatgct agtctaggtt ctgatttctt
    18061 ttcaaattca ataaattttt taaactcttc taatgtccaa aagttcattt catctgcgtt
    18121 ttttttaccg atagaacctg ccaaatgagc aggattagaa ggtaagttat aatatttaac
    18181 tgcataattt aatacagcaa caagctggtt gtttatcgtt ttaatatatg tttggctata
    18241 atcacttttt aataattcgt tttgccatct tcttatatgt gttgcagtaa tttcatttat
    18301 ttttagactt ttaaaaaaag gtaaaattct aaagtttatt aaatactttt tagtttctaa
    18361 tgtagataat tttaatctag aagacatatc gtgcatgtat tcttctatga gactttcaaa
    18421 actcatatca gtactcattt tagatttatt caaaaattct ctttcaaact ctagagcttc
    18481 ttttttagtt ttaaaacctc tcttaatttt ctttttccta tccccatcga agtctgtaaa
    18541 ataaaagcta gcataccaac tttttctttg ttcgtctttg taagcaggca ttttagacac
    18601 ccccaattat gtcattttaa tattacaata attataacat gttagatatg ctatctaaat
    18661 aattgtttta ttttcctctt ctttttattt cttgaagctt gtaatattta cacagttcta
    18721 agaagtcttc ttctagtgct atagataagt ctaatatcgt agatatacct atatctttgt
    18781 actcttgatt ttctagtttt gatatataac ttctgttacg tctcattttt tctgctaatt
    18841 ctagttgtgt cattcttttc ttttttctta attttttcaa catttattaa aatccacctt
    18901 ttaattattt ttatgacatt tttataatta atagtttgtg taatttcttg taaaaaatgt
    18961 tcctcattag aacatttttt ggtggaaaaa tgtgttaaaa tgtaagtaag aaaagcactt
    19021 tcaaagcact ataaaaatag tgctgaaata gataaacaaa taaaagtaat aaggaggaaa
    19081 gatagattct agagggaaat cattatcatt tttaagaacg tatgttcggt tggtgggata
    19141 gaaaaatact ttacagggga tggtttattt gaatgagaat gaaaatatga gtttatatgt
    19201 cagtaagtta aaagaagtat tgaaaaataa ttcagaagag tataaaaaaa taagagctaa
    19261 aataaatgaa atttataatc taaatgaaaa gaaagaataa aagggcttta gctcttttat
    19321 tctttcttag tattaactgc tttataataa gcattatcta tcattttttt tattgcattt
    19381 ttatcatctg aatctaaaga tgctaatcta tttattagtt cgttaacttc attatcttct
    19441 tttgagttaa ctttttttaa taattcatca tcttgttgag tgttaggttg tattttacct
    19501 aatttagtta atagctcctt taaagttaaa tttaatccaa aactcatttt ttcaagcata
    19561 tctaaagtag gttcaacagc tttaccattc cttgggtctc tgtttttttc tatcttatct
    19621 atatatgtat ggctcacacc acaaagatta gaaaattctc taagagataa gttatgttca
    19681 cttctatatt ctttaataat ttctgctaat gtttgcataa tttcacacct cacctaaata
    19741 atagttgact taattatatc agaaaaatgt aaactattgt taacaaaaat aaaaatttag
    19801 taaaaaataa ttgacaagag taaaaaaaag acgtatacta tgattaacaa aaaggttata
    19861 aataaagtta ggagggatta atataaaaaa taatttacaa tatattagaa aagaatctag
    19921 aatatcacaa aaaaaattcg ctgataagat aggtatatca agaccgtatt tatctagaat
    19981 agaaaacggt aaggttaatc cgagtttaga aatagcgcat aaaatctcgc tcgaaacagg
    20041 gaaaacaata aatgaaattt tttttgattt tactgtaaat catagtaaac tataaggttt
    20101 taattgtacc ttgaaaacta aatataaaat attttaaagg agcaagtata tgaaaaataa
    20161 agaaaaagaa ataatcatta gaggaatttt aataggaatt ttttattttt taggaattgt
    20221 atttagtaat acttttttta aataataaat agaaggggta ggtgaaatac caactcccca
    20281 aatcaaaata acactttgga agttaaatat agaatattca aaagaggtga ttagatggaa
    20341 atagagcaaa caacaatacg cctgctaaaa agcagacgaa agttgaatat taatttctat
    20401 tttggttagg cgaaagacct aacaaaaaat ctgtagatac attaagtttg ttagaaatta
    20461 atattaaagt ttctatattt ggctctcttg aacccgattc ataatattga tatgcacgtt
    20521 ctgagatacc aaacaaacta gcaaattgct tttgagtcat attcatttgc tttcttacta
    20581 gcttaatatt atctttgaat ttagtcataa ctacacgctc caataaaaaa ataaataaat
    20641 ttcttgacac gaacaatatt agcgtgtaat atataaaaca tagcacgaac aatattggcg
    20701 tgttaaataa aaaggagagg gtgaaaataa ataacaattt aaaactacaa cgtgaaaaag
    20761 ttggcttaac gcaattagag gttgctcaaa aagccaaaat aacagagaga agctatcaat
    20821 attatgaagc tggcgaacgt ctcccaaaca ttcgtacagc tttaaagata gctagaattt
    20881 taaatactaa ttgtgaaaaa ctctttaatg aataatagca taaaaggact cagattacaa
    20941 gaaaggagct ttagatatga ataatttgca acttaacaat aaaaatacaa taacaacatt
    21001 agaaattgct gatatgttag aaattaacca ttgggaagta ttgagaaaat tagaaggcac
    21061 agaaaaaaca aaaggaatta ttgatattct taacgacaac aattttgttg tggttgatta
    21121 ctttataaaa tcagcttatt tagatttaaa aaatgaaaat agaaaatgtt acaatgtaac
    21181 gaaattaggc tgtgactttt tagcaaataa atttactgga gaaaaaggaa ttatttttac
    21241 agctagatat gtaaaaagat ttaataaaat ggagcaagag ttaaaagaac aacaacctaa
    21301 actaccaact acatacaaag aagcattaca acagttatta atagaagttg aagaaaaaga
    21361 acaattacaa ttagaaaatc aagaaaaaga taaggtaatt cagttacagc aaccaaaagt
    21421 attatttgct gattcggtag cgtcttctga caattcaatc ctagttggag aattagcaaa
    21481 gttgcttaga cagaatggaa ttgatacagg acaaaataga ttatttgact ggttaagaaa
    21541 taatggttac ttaataaaac gtaaaggtga ggattacaat acaccaactc aaaaaagtgt
    21601 agatttagga gttatagaaa caaaagaagg tacaagagta catccagatg gtcatacaag
    21661 tattactaaa acacctaaga ttactggtaa gggacaaata tactttatta ataagtttaa
    21721 aaagaacaat caaatatcaa tgttaggtta aagattaata gcactttgaa aactaaatac
    21781 agaatatttt gaaatatatt gttttaatta attattaact aggaggttaa atacatgaaa
    21841 aataatacaa gcgattcaag agtaaaatat ttttgcaagt gtccatattg tgggtttgat
    21901 aatgaggtag aagttaaaaa agggtcgaag cctaaaatat gttgtatatg tacaaaagaa
    21961 gttgagtatg aaaaattgga gcaacaaagt gttttggaaa ataccgaaat taaagaagta
    22021 tgtaatcaaa tgaaaattaa aaaattagca ctagcaacag gagaagtatt tgaaaatata
    22081 gagctcaaag aatttgaaaa taaaggagaa tgcttaccac caaatatggt aaaggttatt
    22141 gatggtaagc aagaacttct aataaataaa gagttcatat tatcattaga agtcaaaaga
    22201 aagactactt tttaggacct ttcttagttt gtgacaaagc actaccagca accgatttgg
    22261 aagcagcact atatcttccg tctttaagaa tcttactagc cttagaagca acagttttac
    22321 ttgtttgttt agtattttta gctataggta tcaccacctt taaatgtatt tataggattt
    22381 atcctacaaa tatagtatat caaaggaggg aaataatggc aattaatgac aacataaata
    22441 aaattttaag agatagagat ttaaaagcgt ggaaattagc aaaagaaata ggcgtagatt
    22501 caggaaattt atatgcaatt ttaagaggag aaaataaaaa tccaactata gatacattga
    22561 taaaactagc tgactattta gatgttacat tagacgaatt agttggaaga tagaaattaa
    22621 atacagaata ttttgaaagt aggtgtattg tatggcaaaa gcagtagcta aagaacaatt
    22681 gttttatagg gcaaaagata tagctaagat tttagatgta tgtgaagcaa ccgcttataa
    22741 aataattgga gaattaaata aagaattaga aaaagaaggt tggaagacct ttcctggaag
    22801 ggtatcagta acttatttca aagaaagata ttgttataaa acaaaaaaag gggcataaaa
    22861 aatgctatga gagtagctaa agatatgttt cctaaagtaa ttgtataaag aggaaggggg
    22921 tgatttagtt gaatgtaaga gtactgatag cttatataca gttttgtaag caatacaata
    22981 agaaagcgag ttttgaaggt cttaaaaaat acaataaggg gatagtgata taaaaaaact
    23041 taaagtaaaa tttcctacaa aaattcttaa agcaggtaaa gcaatcagag taacatgtgc
    23101 aagatttggt tttgaaagcg attgtataat aactcaatcg caagaatttg aattaacagt
    23161 agtttatttt gataaagaat tagatgattt agtacaatcg tctataacta tagatgatgc
    23221 aataagctat gattattgca ttgaaacgtt aaattaagag gggattaatc atgaaaagtt
    23281 taattatagt gagaaattca gtagagcaac aactaaatag agctaattta gaaataaata
    23341 aaaacgagca actttataca aaacttagaa aaaaagaaga aagaaatata tcagaagaaa
    23401 ttgaattgag taatgcttta agagaaaaaa gtgtaaacga aagattaaaa atatttgctg
    23461 agtcattact aaaaattata gatacacaaa ttgaaataaa agaatatgaa gaaagcgagg
    23521 attacaagat atttgaatta atttcagaag aacttgaaag agatatacct atagatgttc
    23581 agatataaga aaagagccac tgcaatggct ctaatcaaaa atatatcaaa aatttaatta
    23641 gctatattat agcataaatg gagggaaatt atgagtactt tatacgaatt aactacagat
    23701 ttattagaaa tagaagaagg tttaacagaa acaacaggaa atgaagctga aaaactagag
    23761 gaaataaaag aaataataaa acaagagata caaaataaaa acactaggat agtttcagta
    23821 atattaaaca ttgacagtga tataaactct atagattcag agattaaaag attgcaagag
    23881 ttaaaaaggg ccaaaaagaa tactcttgat aaattaaaaa gcaatataaa agactgtatg
    23941 gaattacttg agactaaaaa agtagaaaca attttaggaa atataagtat aagaaagtcg
    24001 gcaggtagct tagtcataga agatgaagaa aagatacctg ctatatataa aacagttgag
    24061 caagttgtaa aagtagataa gaacactatt aaagacttta ttaaaaaagg tcatgaggtt
    24121 gaaggttgta ggattgaata tggaactaca ctaacaattc caaaagctaa aaaagagtag
    24181 gtgaggacca tggaaactaa taatgtttat ataaaacttg taaatataca gagtacttta
    24241 aaagctccta aaagtcaatt taatagcttt ggtaaataca actataggag ttgtgaggat
    24301 atactagaag gtttaaagcc tattctaaaa gaagaaaaag cattggttgt attggatgat
    24361 aatattgttc agataggaaa tagattttat gtagaagcta cagcaacttt aatagatgca
    24421 gaaacaggag aaaaaatatc tgtaaaagca ttagctagag aagatgaaac taaaaaaggt
    24481 atggatttag cacaagtaac tggaagtgta tcaagttatg caagaaagta tgctttaaat
    24541 ggattatttt gtattgatga tacaaaagat agtgatgcaa caaataaaca tggaaatgag
    24601 cagaaaaaaa aagaagttaa tgagagcgaa ttaaatatac tatattcgct aggagaatct
    24661 atagaaaaag ataaaaatag agttgatagt gaagtatata agaagtttgg aaagttagca
    24721 gtagatttga ctaagcagga gtatgagaaa gttttaaatg gatataagag cattttagag
    24781 aagcaaaaac aagagtaggt gatagtattg gggattataa gagtaagcaa agacaaagat
    24841 aatccatatg tagttttaaa taaaacttgt ttggaagatg tgaaattaag ctggcaagca
    24901 aaaggtttac attcatatct gattagtaag cccgaccact ggaaaatcta tgttaatgat
    24961 ttatataaaa gaagtaaaaa tgggagggat gctacagcaa atattttaag ggagctcata
    25021 gaaaatggat atataacaag aacaccttgt cgagattcta atactaataa gatgcttgga
    25081 ggatatgatt atcaagtata tgagatacca cttgaaaatc ctcagaagct aaaatcccga
    25141 aaaactgatt tcccggaaac cggatttccc ggaaaccggg tttctcggaa accggaaaac
    25201 acggaagtag taagtaatga ctttaaagta aataatgata ttactactat tgttattaat
    25261 gaacaatcca ataaagacaa aaccacctac ataaaaaaat actttgaaaa atatataggt
    25321 gtgattactc ctaataactt tatagagtta atgagttact tagatgatgg aatggaagct
    25381 gatgtaatta taagagctat tgatgaagca atagcaaatg gagttaagaa ttataagtat
    25441 gtaaagacaa tattaaataa ttggatagaa gcaggtgtaa aaactaattt agaacttaca
    25501 gagtatcaaa atgagtttga gaggaagaaa aagaataaac aggataagaa gcagtctaat
    25561 agtaaagctg tgaatactcc taatgtgagt aaaaataagt ttcataactt caatgaaaca
    25621 tttactcaat attcacctga cgaactagat gacataatta agaaaagcca aaaggttaaa
    25681 tttaaataaa attaaacttc taggaagtaa atatcaatat attgcttcct agaaaagggg
    25741 aggtataaaa tggcgagaat atatgcacaa agaagtggtt ctttaaatga acaagataga
    25801 ttggagttat taagattact tgggaaagct ggatatacag taaaggttgc tagagagaag
    25861 caaaatagca agacaactta tacttacttt gttgagtata cagaagaaca ggaagaaaaa
    25921 tagaaggggg ctagttaaat gaatacaata actttagttg gaagattagt tgcagatgca
    25981 gaattgaagt accttccaaa ttcaggtact ccaaaaataa ccttttcaat ggcagtagat
    26041 agaaggttta aagataaaaa tggaaataaa ataactgatt ttattcaatg tgagcaatta
    26101 ggaaaacatg tagagaatct agtgcaatat cttgttaaag ctaagcctgt ttatgctgtt
    26161 ggagagttaa atatatataa ttacaaagat gaaaatggtt gctggaaatc tattactaag
    26221 gttaatgtaa atgctttaga actactttct agtaaaaatg ataataatgc taaacaagaa
    26281 tatgtaccac caggattaga cccacaaggt tttcaagcaa tagatgatga cgatatacct
    26341 ttttaattaa gttaaatagt ctagggagtg attatacaat attacttcct aggagttgaa
    26401 aaatattgga ggtctaagag tgaaatatga gtgtgagaaa gtgttcttag aatgcgataa
    26461 gggaagtttt gagataaatg atacaagaat tgaagaagta acattcgagg gtacagaaat
    26521 agacaatccg tttgagagag taaaatatga agggactttt acaataatat ctggatggga
    26581 ttctttattg agagatatat tgtggcttga aatgcggaaa gtcttaaaaa ttataacaag
    26641 aaaaatgatg atgggggtat taggaatgat aataattaga agtcaagata gattagattt
    26701 aatgagagtt aacagagttg aaatatacga taaacaagta tttgtaatat ttgaagatga
    26761 tgtcaaacga ataggtgtat atgaaagtaa tgaaagagct attgaagtat taaacagaat
    26821 acaggaggct attattgcag gaactaagtt tgacattata aataaagacg gggttagatg
    26881 caacaaagaa aaagtgtttg aaatgccagt tgaataagga gggagctgaa atgttaaagg
    26941 ttgaaaaata ttttaatggt tctgttgcag ataacatatt tgaagatgat ttgactctta
    27001 gaaactactt agcactatat tgttgtgttt atggagtaag aaaaaatgga gagcttgtat
    27061 ttccaacttc tgaaaaaatg ctaatagagt ttaatgttga taaaaataga aaaaagaaaa
    27121 agaaaggttc aaaggtaaag ttgattaatg ccaaaactgg tgaagaaaaa atatttgatt
    27181 ctatagatag tgcagcgtgt tttttaagac tacagagtca ggcagtttac caaacaatta
    27241 aaaagaaaac taaaacaaga agtggctgga aagctgaata tattaaggag gaataatgga
    27301 agtttcaagg actgaataca caattaaaag agcaaaagag ttgtatgaca atggagagga
    27361 catatttatt gctatagata aggctagaga agaatatgag gagatgatta aaagtgagtt
    27421 taattaagta cagaggttat gattttgaga acgaaaagtg gatttattca gcaacaataa
    27481 tgtggagtaa tatacttgaa tgtttagtta tgttgacaga gggttgcaaa tggcagaaag
    27541 tctctaatgt tggggtatgc tctggagaat gggctagaaa caatcaagaa atttgtgaag
    27601 gggatatatt gaaaggatat gataattcta gtgatacaag ccaatatgga gttgtaaaaa
    27661 gagattttaa tagtattaag ttatatttgg aatggcatta tttaaagaag tttgaaggag
    27721 aatggataga gcttataaat aaaacagaaa tatatcatag tagagattac aaagtaattg
    27781 gtaatgaata tgagaattta gaggaagtca gaacagagtt cttagaacgt aaggagagtc
    27841 ttgaaaatga atatcttagc tagtgcgata ttagtaatag gaagttttat aactggtaga
    27901 gtttatgagt atagattgaa tctaaaagag tgtgaaaatt gcgacaataa aagaggtgta
    27961 taagaatgga tgatagatta gaaatgataa atgcttctgt aaattatata cagatgatat
    28021 gtgaaagttc aaatatagct attatagcag agcgaggaag agttagaata ttagatttag
    28081 aaactaaaga aaaatatgat ttattaaaaa ataaactcga ggaaatgtta gaagaaatat
    28141 aagtgaaaat atctaattaa gacagtttag agagttacaa aatatctttt agtataaatt
    28201 attgttgaag tgttttgtga ctctcaaaaa tgaaaataag gaggcgttgt attgcttaca
    28261 tttttagatt tattcgcagg gataggtggc tttaggctag ggatggaaaa agcaggacat
    28321 aaatgtttgg gacattgcga atatgataaa ttcgcaaatt taagttataa tgccatgcac
    28381 aaaccgaagg aggatgaatg gtttgaaaga gatattagag aaattagaac agaaaatatc
    28441 ccaagagcag atgtctggtg ttttggattc ccatgtcaag acatttctgt tgcagggaaa
    28501 caatttggat tcagaggaga acgttcaagt ttatttttta cagttacaaa acttattaga
    28561 gaactcaaag aagaagatag acccaagtat ttacttattg aaaacgttaa aaatctactt
    28621 agtgttaatg gaggatttga tttcctcaaa gttctcgttg aactggatga aatcggctat
    28681 gatgcagagt ggcaagttct taattctaaa aacttcggag taccccaaaa tagagaacga
    28741 atattcattg ttggacattt tagaggacga agtacacgaa aagtatttcc tatcgaaaga
    28801 aaaagtggaa aaaatcttga gcaactaaat aatccaactc atagtacaaa tagaatttat
    28861 gatgcagttg gaattgctag atgtattaga agtcaggcag gaggtggagg tgctaaaaca
    28921 ggtctatact ttatagactt aaataaaaac tctaaagtaa caataaatgc tagatgcctt
    28981 aaagcaaaat ataatgcagg tgtgacaaat agaaattgtg ataatagtgg agttttagtt
    29041 aatgcagttt taacgcccga tagggtaaat aaaagacaaa atggtcgtag aattaaagaa
    29101 agcggagaaa caatgttcac attgacagct caagataaac atggaatttt gaaaaatgga
    29161 gatataagaa ggttaacacc aaaggaatgc tttaggttgc aaggatttcc ggataaatat
    29221 tacgaaagag cagcaagtgt atgctcagat agtcaactgt ataagcaagc aggaaatgct
    29281 gttactgcaa atgttgtata tgaaatagca aaaagaatgg gctaaaagtt gcaaaatgtc
    29341 ttttagtatg aatatttttg aagtgttttg taactctcaa aaatgaaaat aaggggtggg
    29401 ataaatgtat gaatatatat taagatggca aataggatta tcgttagaaa atagaaaaat
    29461 acattataca tatggaagta aagaagcttt aagaaagaaa gcaaaggcat tggctaaaga
    29521 tgaaaatata gtactaataa ctatagataa ggtagatgaa gttataaaaa atactataag
    29581 cgagaagatt atagaacgtt ttgaaaattt ataaggggtg gaattatgat aatacacaaa
    29641 tttataatac atgttttaga taagaatagt gatacaccaa tattgaatga ttttgaaggt
    29701 agggttaatc aagatatggt cctatttttt caaaagaaaa taagcaaagt atcaagagat
    29761 aatgacatca gaacagcagt atttaataac tatagtaaca atctaattaa gaagtgttgt
    29821 gaacaaatta tttatgatga aagttcattt ttaaataact ctaaagagat tgcagcttat
    29881 ttatttgatg ttatgaaatt gaatgctaca ttagaatctt gcgacttagc aatttgttta
    29941 tactctcaaa aagatgaaaa gaaagttgct atattaaagc ttgattacaa taattcgtat
    30001 actcattcaa ttgagtttaa agatgataaa tttaatatac agatgtctaa aaatgaaatt
    30061 aatatacaag agactaagac ggttaaaatt gctgctttgg ttggattgag tggaatgaat
    30121 gacaaatatc atcttagggt tttagacaag gatgcagaga aggaagaagc taattctaag
    30181 tttgttacag agttcttaaa tgccactaag ataaaagatg ataagtataa gactaagaag
    30241 ttcaaaaata cagctgagaa ttggataact aatgctctta gtaatgatat aaaacaagca
    30301 gaggatgtaa gaagtatatt aaattatact ttgagagaaa agcatgaaat tgatataaat
    30361 gattttgttg ataaaacaat taaagatgat aagttaaaag atagttttaa agaacatatg
    30421 gaagaaaaag gtcttgttga aggatttagt atagataaaa aatgggttga taaaaagctt
    30481 aaaaagagaa atataaaaac tgacaatggc tttgaaataa aaggtaactt aactgatttt
    30541 gaggacccaa tgaaatatac agtaagacaa aatcaaaatg ggtctataga tatagttatt
    30601 aagaatgtaa cattttatga ggaaaagtag gtactcatgt gactattggc tagagaagga
    30661 gaagtaaata ataagaggat gtaaatttaa actagttagg aggaataact tatgaagatt
    30721 tttttattga ctatactgct aataattatt tgtatattag caaattatgt gaaaaatcgc
    30781 atatataaaa aatctataaa taatctaaaa tataaatatt ctgtagggga aaagattata
    30841 tatcatcaaa taaactgtta ctataacaga atggttggtt gtgaaatttt agaaaaatgt
    30901 tatagtacga aatttagaaa aagaaatacc ccgctttata aagtaaaagc atatgtaggt
    30961 gataacgata caacatgggt tataccagag tggagaattg aatgtcttgc tacgacttat
    31021 ggagaatttc ctaaatatta aataataaaa attggctgga gaaggagatt gtaaattatg
    31081 tttaacatct ataaagtgaa aataaagact aaaagaacat tggagcaggt aagaaatcaa
    31141 agcgtagact ttgagtattc agaaaaagga ttaaaaaata ctctgaaata ctataacttg
    31201 attgatgatt taaaagtaat agtagttaaa tttggagatg aatattgtct agctaattac
    31261 aatgaagaag atagaaaaat aataatggaa gcacattatc ttttagagca ggatgaatat
    31321 actggatgtt atataaatga atatgaacga tttaaaaaag attgggaaaa tggtaattgt
    31381 gatggggaag cctgtatggt attttcagat gatgaaattg agataattga gaagctaagg
    31441 gagggttaaa tatgaataaa agaattaaaa tgaaaaaaag attaattcat aaaaagtgtg
    31501 atgaaagatg tgtcaactat gactttgtaa ttagcaataa ccttataact tgtaatgtgt
    31561 gtataggatg caaatacaaa gaaaatatgg ataaagtatg tgaagagaac tataagaaat
    31621 taagaagtaa atagaataaa atagtcaagg taagtttgtg aatgaaacta gaatgttata
    31681 ggcttgactt ataaaaggag tgcgttaaat ggctaatata tattgtgaaa attataattg
    31741 taaaaactac tttgaagata tgtgtatgct tgaaagaatt gaaattaata acttcaaaga
    31801 atgcgaaagc tatcttgaag gtaaaaatga gctatatgaa ttagaaaacg gatatactat
    31861 acatcctaaa gatttgaaaa tggtgaaaag taaagattat tctgttgaag ttactcatat
    31921 tccaactggt attacagtaa aatgccgttc tacaaatagt attttaaaaa ataaaaataa
    31981 gtgtttggaa gttctagaag aagaactaac aaaaataaac tctcacttag agctagaaga
    32041 tttacgctaa ataggaagtg agcttatgaa acgaagaaga tgcagttggt gtggtaagtt
    32101 attttatctt gaagaaaaat ctaaggatgt ttattgttgt aaagaatgta ggaagaaggc
    32161 taagaaggtg aaaaaatgaa agtttttctt gtaatagatg gagaaccagt tggcaaagaa
    32221 agacctagaa tgaactctat aactaaaagg atctatacac ctaataaaac tagagattat
    32281 gaggagttaa taaaatggct gtatcaatct aaagtgaagt atcgttttac tggttatata
    32341 aaaatgactt taagatgtta ttactctata gctaaaagta acagtaaaaa ggttaaggag
    32401 cagaaaagaa ataatgtgtt aagacctagt aaaaaacccg atattgacaa tgttgttaag
    32461 attatagctg attcactcaa tgagatagct tataaagatg atacacagat tgttgaggtt
    32521 gtagctagta aatactatag tgataagcca agggttgagg ttatattaga agatattatt
    32581 taaggaggac tataaatatg aatgagatta tgacaaatga aaatttaaga gttgtagcag
    32641 atgatttagt tacagtttat gaaactgata caggagagaa aatagttttt gcaagagaac
    32701 tacataataa tttagaagtt aagagacagt ttatagactg gattgaagat agaattaaac
    32761 aatatgggtt taaggaaaat gaggattatt cagtttttca caaaaatatg aaaaactcaa
    32821 ctggtgggag accttcaaaa gaatatgtat tgaagcttga tgtagcaaaa gaacttgcta
    32881 tggtacaaaa caataataaa ggaagagaaa taagaagata ttttataaga ttagaaaagt
    32941 tactaaatag aaccttatca aattcacagc ttagtcaaat taatactatt gtgaatgaat
    33001 cattacttaa gatggaagtt aaacataata tccaaataga gcaacttaaa aaagagtgtt
    33061 cagaatatta tagaccaacc tctaaaacta aatatgatat atcttcttat ataaaagaaa
    33121 gactaggtat atcaaaagtt aatgaagaat ttgaattagt taagaaaaga actctattgg
    33181 tattaggtgc tgacaaatgg gaggatatcc cgaaagatgt attacttaat tcattaaact
    33241 taatagatga atcaatcaga attataaaat ctgagagaaa aacaaatcaa attagtttct
    33301 ttgaaaaaga taatttctgt taataaaaag aaaaaaggag tgctttcaca ctccacttgt
    33361 caaaaatata aagcttttat ccaagattat tataacataa acaggagtgt ggaagtatgg
    33421 ataataatat caataaaaaa gaactattta aaaaagtaga aggtagacta catcattata
    33481 aatttttaag tgcagaaatt aaaaatcttg aattagatat agaaagtaga gaaaatgaga
    33541 tatttgggtg taaggctgtt ggatatgatg aaaaagtaag tccaacatat gcttttaatt
    33601 caagtgtgga gaatgagatt ataaaaaaag aaagagatat tactagattg aaaaaactga
    33661 aaaaagataa ggaaattgaa aagaagaaaa tagaaaatgc acttacatgc ttagatataa
    33721 gagaagaaca tttttttaaa ctgttttaca atagcagaat gaaaaatagt atggtttata
    33781 tatccttaga gatgaactca gatagaaaaa catgtagatg tgtgagggaa agattagtgt
    33841 ataaaattat ggatatgctt tatccaagaa ttaaggaaaa tgaactacca ttatttaaaa
    33901 attagaaaat tccccagttt ttccccagaa attcccactt tattccctac tttctcccct
    33961 ttttgattaa aaaagcatga gataataata tcgtggaaat aaagatttcc ctctcaaaac
    34021 taaataattg ctaggttagt ttaaagggct aatctagcaa tatgaacaga ctaggcaggg
    34081 cgtgaggacg ctgtaagttc aattctaact atgttcaaaa cctattaata cactatatgt
    34141 agtagttgaa ttaagattaa aatctcatac aattttgtat cttaattcag aagtctaaaa
    34201 accgagtggg gcttggtaac ctcactcacc atgcaagtac tggtttaatc taggttcgat
    34261 tcctggaact tgctcccttt aataatatgt atcccccatt aaaaaggctt agattaactt
    34321 ctaggtcttt tttaatacaa aaatttatta aaaatgcacg gcatgcacta ttcagatatg
    34381 catgtcatgc atgtctaaag tcgaatattt aatattttcg atggctcaaa ttgaggggtc
    34441 gaaaataaaa acaggaggta gtagtatgtt gaaaatttta caagagaaaa atgtaaaagt
    34501 aatgtggtcc aaaaatggag aagaagtttg gtttaatgca aatgacgtag gagaggaact
    34561 aggcatagta aatattcgtg atacattaag aaatatagat agagaatata aaaagaaatt
    34621 taatgagtct actgtcggag attcctacac tagaaacttt aaagataaat tgcctaactt
    34681 cggtactact tttgttacag aagaagctgt gtacaatatg tcatttagaa gtaataaacc
    34741 agaagcaaag ttatttacaa aatgggttac aaaaacactt aaacaaatta gaatacatgg
    34801 ttattatatt gctacagaaa aagaccagga atggctggat ataaggacag aaggcaaaaa
    34861 agtaagaaaa gattttacag atgaaataca agagtttgta tattatgcta ctagtcaagg
    34921 tagcaataaa cctcagatgt attataaaca ttttactgaa cttgtaagaa aaaaattagg
    34981 tattccaaaa ggtgtgaaaa gagatgagtt aaatcaaagc gaactatttg atatacaagc
    35041 acttgaaaga attatatcta tgaaattacc taagttaata gataaagata tgaattataa
    35101 agaggtatat aaaaagatta aggaattaat agaaatgatt taagggactg tcttgatgga
    35161 gggtcttttt taattcccaa aacgacaaac aaacgaggtg gtgatgtgca agatgtcaaa
    35221 gaaaaggtaa aacaagatta cataaaaggt atgaaacaaa aggaaatatc agcaaagtat
    35281 gacattagtt taaacacttt aaagtcatgg ataaaaagat acaattgggc tagtgagaaa
    35341 aagaagggtg cacctaaaaa taaaagaggt gcacccatag gtaataaaaa tgccactggt
    35401 cctcctggaa ataaaaatgc tgaaaagttt ggtttcttct caaaatatct acctgaagaa
    35461 actagggaat taatacaaga aatatctata aaagataaat ttgatattct ttgggaacag
    35521 ataacaattc aatatgcagc aataataaga gcacaaaaga taatgtatgt taaagacaag
    35581 gaagaaatga ttaaggagtt aaagaaacat gaaagcacag aaaatggtga gaagatagag
    35641 tatgaatttc aatttgcatg ggataggcaa gcatcttttc ttaatgcaca gagtagggct
    35701 atgagtgagt taaggagttt aattaaacag tatgatgaaa tgattcataa ggattggaat
    35761 ttagctacag aggagcagaa aaatagagtt gaaaagttaa aatgtgaagt tgataaccta
    35821 aagaaaagtg atactggaga tgattcaaaa atttgggttg aagctataca aaatattgca
    35881 atgaaacgtg gtgttaacaa tggataaagc tttattgaca ctattagatt gttattggga
    35941 taatcctgtt tggtttgcag aggatatgtt aaattttaaa gctgacaagt ggcaatctga
    36001 tgttctgatg gctttagctc aaaccccaaa agtatctatt agaagtggtc aaggagtagg
    36061 taaaactgga ttagaaagca ttgcaactgt atggtattta agcactagac cttttccgaa
    36121 agtagttgct acagctccaa cacgacaaca attatatgac gtactatggg ctgaaatagc
    36181 taaatggcta agtaatagca aggttgagaa gctacttgag tggactaaaa caaaagtgta
    36241 tatgaaaggc tttgaagaaa gatggtgggc tacagctaga acagcagtaa agcccgagaa
    36301 tatgcaaggt tttcatgaag attatatgtt atttgttgtt gatgaagctt cgggagttgc
    36361 tgaccccatt atggaagcta tattgggaac attatcaggt gcagaaaata agcttctttt
    36421 atgcggaaac ccaactagaa cgagtggaac gttttacgat agccataata gagacagaga
    36481 tttatataaa acatttaaag tatcttcttt agatagccct agaacatcaa aagataatat
    36541 tgaaatgcta aaaagaaagt accatgaagg ttctgaccct tggcgtgtta gagttcttgg
    36601 agaatttcca aaaggtgaaa gtgattcttt aatatcttta gaagctgttg aaacaagcac
    36661 aataagagaa gtgaatatat ctaatgacta tatattaaat ataggtgcag atatagcaag
    36721 atatggtgac gatgaaacta taatagctcc acgaataggt ggaaaagtgt ttgatttatt
    36781 aacttattct aaaaaagaca caatggaaac tgtaggaaat atattaagag cagttgataa
    36841 atttaaaagc atgtatcatc aaatcaacag agtaaaaata aaaacggatg atgatggttt
    36901 gggtgcaggt gttacagaca gattaaaaga ggttataaga caagaaagac tcaaatatga
    36961 aattgtacct attcaaaatg gttctagtgc tatagaaaaa gataaatact ataataaagc
    37021 ttctgaaatg tgggataaca tgagggagga attagatgca aatttaagtg gcttcataca
    37081 aaataaagaa gctataatac agcttcctaa tgatgataaa cttattaagc aactatcaaa
    37141 tagaaaatat acagtagatt caaaagggaa aatacaaata gaaagtaaaa aggaaatgaa
    37201 aaaaagaatt ggagaatcac ccgatagagc tgatgcagta atatattcgt ttgcagaaaa
    37261 taacaatact gatttatctt tactgaaagg gggtagtgta tggggataat atcttatgta
    37321 aaaaagctat ttaaaagacc tactggagaa attatgcgta tgtctagtgg aaatataggc
    37381 gtatataaat tagatgattc tagagttgat tatgagttag caagagaatt gtatcaaaac
    37441 aaaaatgcta attataaatt aggttctagt tttgttagac caattgtcaa ttcaacaact
    37501 ggttttatgg gtgtacctca ttttcaaata gaagatgaag aagctcaata tatattagat
    37561 gaatttgttt tagataacac atctaaaatg ttaaaaacac acacagatag tttaaagcaa
    37621 ggtgattgtt atatttggat aactagagaa gaaagagaaa atcctttata tcctgataaa
    37681 aaagttagat taatatataa cttcatatca cccgaagaag tgaaagaaat aatattagac
    37741 cctacgacaa aagagcctat agcttatata ttagaaagcc aaaatgaatg gactgactta
    37801 gaagaaaata agagaagggc taaggtaaaa caaataataa ctgctgaaag tagaattatt
    37861 gaggttgaag gggataagat agaaggttta gaagaagggg aaactcctaa tgtatggggc
    37921 tttataccaa taatacattt taaaaatgaa gctgatgaaa cattgaaata tggtcaaagt
    37981 gatatagaac caatagaacc tcttttaaaa gcttatcatg atgttatgtt acatgcgtta
    38041 aaaggtagca aaatgcactc tactccaaaa ctaaagctga aattaactga tgttgcaagt
    38101 ttcttagcac acaactttgg tgttgaagac ccagttaaat ttgcaaaaga gggtggaaag
    38161 ataaatcttg atggacatga aatactattc ttaaataaag atgaagaagc tgagtttgta
    38221 gaagtaaaat cagctatagg tgatgctaag gagcttttaa agcttctttt ttattgcata
    38281 gtagatgtat ctgaaacacc cgagtttata tttggagtac atacacctag tgctttagct
    38341 tctgtaaaag aacaaatgcc tattatggta aataagataa gaagaaaaag agaacaattt
    38401 acaaatagct ggcaattact tgcaagaatg gttttaataa tgagttctaa ttctagtggt
    38461 atgaaatatt catcttatga tgtgactata ggttgggatg aagtaaatcc gcgtgatgat
    38521 aaagaattag ctgaaacact agaaaaggta tgtagtgcat tagataaagc tttagaaggt
    38581 ggatttatta gcgaagaatc aacagtaaac tttttagctc agtatataga tacaatgagc
    38641 aattatataa gtgatgaccc tgaaatagtt ggagaaagag aaaagataat aaaaactaaa
    38701 atgttaaaat acagattaga tgattctcaa ggtttagatg atgagtcaaa tgaaattgac
    38761 aaggaaatag ataaaataaa ggataataat gacaatggat aaaagtactt tggaattaat
    38821 aactgttgca ggggagtaca agaaatgggc attagaagct agaaaaaaat ttatagattt
    38881 aagactcaag caagatgatg aaataagaac aatgtatatt aacataacaa gaaatattac
    38941 aaaagaaata agaaaaggaa atctttcaga ctttaacaaa gttaggttaa aacagatact
    39001 aaaacaatta acacaagaaa taaaaatatt aaatgaacaa ctagtattta attttgatga
    39061 atacttaaat aaaaatgttg aaacagctac tagttactct aaaaatattt taattaatgc
    39121 agttgagaca gctcaaataa ctaaagtaac taaagctatg atacaaaaag ctttctatga
    39181 tattaatata agaactgtag aagcttatta tacaagggtt aaggatggtt tatttttatc
    39241 tgatagaatt tggtctaagt gtaagaagta cagagaagat atgaaagtta tattacaaac
    39301 agcagtaaca gaaggtcaag actgtgttaa aacagctaag atgctagaca aatacgtttt
    39361 aaaaggtaag aaaactttag ttgacgaata tccaaatatg ataaaaagaa taggaaatag
    39421 agtacctcaa aatataagtt atgaagcttt aagattggca agaactgaaa tgacatcagc
    39481 ttatggtgat ggggtcttag cttctgcaat gattaaccct gcaaccatag gtattcagtt
    39541 tatgttgtcc atggcacatc ctcatacaga tatatgcgac gaaatatgtg gagaggataa
    39601 ttttggtttg ggtaaaggtg tttatcctat aaatgaagct cctgcatatc cattccaccc
    39661 tcattgtttg tgtattatgc ttactgtagt tcaaccatta gatatattag ttggaaggtt
    39721 gaaaaattgg attaaaaatc ctatgaatga tacacctctt gaaatgtggt atcaagaggt
    39781 gtatggaaat ttgaattttt aaattgaaag gtggtgatta aatgaatgta ataactggag
    39841 aaatggactc aatgaatgcg ttaatatcta gtataaaacc ttctgatatt cctttagcta
    39901 aagatataga catagaagct ttaaaatcta tagatgatga ccctcttgag gtagttgttg
    39961 agataccagc tacaaaatct aaaaggggat ggaactatac tgctaaaagc ttgaaagata
    40021 ttgtagatta cactaatgaa aatactctta atggtttttt aggacatcaa aaagctgaaa
    40081 atatatcaac tgaatttgca ccacctgtaa cgcattggat aggtgcagaa atgaaagggg
    40141 ataaagctta tttcagagga ctgattgatg ctgatgcaac aaacttgaaa agatggatta
    40201 gaactaaaag gataaaagaa gttagtatat ttggttatcc aaaacttaaa aagagtgcta
    40261 aaggcgaaat gaatgttata ggatatgagc cactatctat tgattggact cctctacata
    40321 gaccaggtat gccaacaagt attgtaggta tggaaatgag tcctaatggc gaacagttag
    40381 atggaacttt tgaagcttta agaatagatt taagagaagc tttaaaagct aagttttcta
    40441 ttaatgataa taattcatat ctctatatac aaaacataag atatgataac aatactgtca
    40501 tatatgagtt ggagcaaaat ggattatgca agctttatag tataccattt actatagttg
    40561 aaaataaaat aaatctaggt gaagaaattg aagtaataaa gaaaataagc tatgaagcta
    40621 aaggagaaat gaaaggagag gaaaacaaat tggaaggaaa agagttaata aaaaatgtca
    40681 aaggattact gcaaactggt gaaatatcat attcagaggt catacaagga ataggcttaa
    40741 ctaaggaaat tgtgacagga gagatggaag atgtaaaaag ttcattaaaa gcagaaaaag
    40801 aattaagaga agtgaaaaaa gtacttggaa tagtaggaga gatggacaca gttgaagtgg
    40861 caaaaaaggc ttcaaaagct ttagaaaatg agaaaaagga agcttggaac tgtatagtta
    40921 ataaagtaat taaagataaa gtgtcaggtg aaatagctca aacattagtt aagaaaatgt
    40981 taaatgttga ggaaggctca agtgaagaag taataacagg agaaatagaa aatatattaa
    41041 atgatgagtt tgtaaaaaat acaatgtcta atatgtataa agataatcca acaacaacag
    41101 gattattaaa ctctagcaat aatggaagtt taacaactaa gaaaaataga atataaagga
    41161 gtgatgttta tatggcattt aaaggtcaac caacgccaag cacaataaca cagataacaa
    41221 gagcaaaaat aagtgatggg aaatctgtaa gagttattct ttcagaaggt gaaagcacta
    41281 aaacacaaca attttatctt ataaatggat tctttggagt cgctatgcaa gacggagaaa
    41341 aaggcgatga agttactttg caaatagagc aagctgaata cgaaacggat aatattgtta
    41401 catcagaagc ttttgaggca gggaaattga tttattggga taatacagct aagaaattta
    41461 ctactacatc tgcaagtaat aggctagttg gtagagtaac agatgggaaa gacagtaata
    41521 atgtaatttg gtttatatta ttacctcaac aatagaaaag gagtgataaa tatatggcat
    41581 ttaaagtaat tagtcaggaa aatttgctgg aacaaaaaag aaaagaaact ttacaagaag
    41641 atataccatt tatagtaaat ggtgaaatgg aatatgtaac aaagaaaata tcaaatggag
    41701 aaatggaaac cttggagtta aataagccac ttggtgaaat gatgactttt agctcgactt
    41761 caaatttaaa agagttatta agaaaagttg tattagatgt tgaactaggc agagagcaag
    41821 tacaactatt atataaacca atctatgaca gtatagcaga ttctaattta ccacaagtta
    41881 tggatgctaa gtgggcttta caaggtaact gtgtattcct agagcatata gaaggtgaag
    41941 aaattaaatt cggtacaata aatgcagaaa atggtccagt tgcaaggata caaacttatg
    42001 caactggttt tgagtataca aaagaaatga aggattttaa ccaaacattt agtgttgaaa
    42061 tattaaataa atcaattggt gagagttaca atgccttgtt aaaccacata catctaagcc
    42121 caataataaa ttttaattat aaagcttcta ataagacagc ttttaaaggt gaaactaatg
    42181 acccaatatg gctaggaatt tggagaacat taacacaagc acaaaaagat acagttatag
    42241 caaaaagaca aggtaatata ttaatggctt ctagtgctga ccaaattgaa atagaaatgg
    42301 cgttaaatgg aggacattta ttaaacggaa gcatgtatcc atctataaaa aatatatcaa
    42361 cagtaattta ttatgatggg tgggaggtta ctgttggtaa aaaaacatat tcttacaaag
    42421 gtgttacacc aggcaaagga tatttgataa gacctaagcg aggatttaaa gagttaataa
    42481 agagagattt aacaacagag gttggaaatg ctgatttaag taagttagta gaaaatcaaa
    42541 ttgtaggtca ttgttataga ggtgcttttg cagcagtaga agaaaatgta caagaaataa
    42601 gttttagata aaacactcat aagagtgtta tttttatgag gtgataatat atgacaccaa
    42661 ctagtaattt aatagaaaaa ttaagactat tattaaatga taaagataaa aaatcattta
    42721 cagatgaaga attaaacttg tttttagaag aagcagactg tatttactgt gcagcttctc
    42781 aaggatgggt attaaaatct ttacaatatg aaaatacagt aggggaaatg tatgagtata
    42841 aagtgggtca agaaacatat aaaagctcta gtataaagga cctagtatct gtagcttatc
    42901 aaaatgcaga taaatttaag gatatgtgta ctaacaaaaa agaaaaggga agttttatgt
    42961 taggaattag cacagaattt gaaatatgat aaatattgat agaagaagaa aagatataat
    43021 aagaactatt aatataaacc ccactaatat tactataact agtattaaaa aaaccgaaat
    43081 agatggagct tttgaagaaa ctgaaacaga aataaaatgt gttgttagaa tatttaacga
    43141 aaagacagca gagaagcaaa tatcaagtga aaagcaaggt acatttagtt ctattagaac
    43201 atatggaatg ttagtaagtg atgatgttgt cttagatgtt aacagtagag attctttgga
    43261 gtttgagtgc atatatggga gaatgaaaat agttaatgta tatcctcaaa ttgtaaaagg
    43321 agaactttgt gggtatcaat gttcacttga aaggattgat taaaatgagt gctttcacaa
    43381 atgcaataaa tgatataaat agaaaaaaag caggtatgtt tgtactttgt atgagtgcaa
    43441 gtgcaatgct agaaggtgaa gctaaagcaa atgcacgttg gacagataga acatcacatg
    43501 caagacaaag tttaaatgct aaaacactta gtggaggaaa taattttatc attagattat
    43561 ctcatggtgc agaatatgga gggatacttg aagaaggctc aaaaccacat gttattactc
    43621 caaaatcagc tcaagcccta tactggagag gtgcttcaca tcctgtaaaa tcagttcaac
    43681 atcctggtac aaaagcaatg cctattataa aaccaactat tgataaaaat ataggcaaaa
    43741 taggtaatat gatttttaga tattggagtg attaaatgag ggcaggaata agaaaagctt
    43801 taatagataa tataaaagaa ttgaaaggtt gttatgaacc taatgtacca aacaaagata
    43861 ctaaaaagcc ttatatggta gttgtacaag ggcaagacaa tgacaatgga gaaacgatag
    43921 gttttgaaag aagtatagaa gtatggattt atgaaggtag aactacattt aagaagttag
    43981 ataaattaac taaacaagtt attgaaatct tagacatgaa tactatagtt gatgaatctg
    44041 aaaatgaagc ttttacttgc atttataaag gtacaagtga aaatgatatt gttgttgagg
    44101 aatgggatgc tatagcaaga ggtataaggt ttagtgtaat agctttagaa gataaagaag
    44161 atacaactaa tgataggtgg gtagaagctc tatctaggca cacaaaggat ttattagaaa
    44221 tagagagtta taaagataat tggaagaaaa actttatagc tccatgtgca ttatggcgaa
    44281 ctacacatat tgaaaataaa agaattaact atcatttaat tgagattact aaaactatga
    44341 aatgtcatgt tgtaagtaaa aataaagatg aaatagttaa gcttcttgaa acattagaaa
    44401 caagcttaat aatagataaa agagtaagac ttagagaaga taagaatatg tatttaactc
    44461 ttgttagcgt agttgaggat agggaatcag atatgtttac aactggacaa ttaacagctg
    44521 tgtttaaaat gataggaaag ataaaaagag aaggtcctac tatggacaaa atttatggta
    44581 atggaaattt aagataggag gtgtaagggt tggctgaaac aaataataaa aagattaatg
    44641 tgagtaagca ggaagaaaaa tatttaaaaa gtgattttat agaaaatagc gaagcacttg
    44701 gctacaagaa agaagtagtt gcaggtgctt tatttaattg taagaaagaa gaacttacaa
    44761 agtcagagtt tgagaaagca ataaaagagt ttttagaaag agaggtgaag taaaatggca
    44821 actggtacat ggaatgaaaa agaaaaaaag gagatacctg gtttttacaa tcgctttaaa
    44881 acacaagcag aaaagtctac aaacacagga ttaaagggta gattagcaat gcctattagg
    44941 gctaattggg gagaagttgg caaggttgta acaataaaaa atgatttgag acaacttaaa
    45001 actctatttg gtgatgatat gaattattca gctttcaagc taggaaaact agctttacta
    45061 ggtaatgtaa aggaattact tttatatagg ctcgtagatg ggaaccaaaa aaaaggtaca
    45121 ttaacactaa aagatactac agagaatagt gcaaaagatg taattaagct agaaactaag
    45181 tatccaacta gcagaaattt taatgtaaca ataaaatcta atctagtaga tgcagataaa
    45241 aaagacttta tattctttga aggaactaaa caattattta gttcaagtgt caaagggact
    45301 atagatgaaa tagtactaga aataaactca aatttagata atgagtatgt aattgcaact
    45361 aaagtagctg atagtgatac agcactagca aacttggtaa atgtagcact tgaaggtggt
    45421 aacgatggtt gcacatctat tactaatgag tcttatctaa aagcactaga agaatttgaa
    45481 agatatagtt ttgactcttt tgtacttgat ggtgtggctg aggaagcatt gcaggaaact
    45541 acaaaagctt gggtagctaa gaataaggag ctaggaaaag atatattatt atttcttggt
    45601 ggaaaagcag aggataatat aaaacaaatt aatgataaat caaaaagttt taatgatgaa
    45661 aatatagtta atgttgggag ttcagcttat tatgagggaa taaaatatac acctagtgaa
    45721 gtagctgttt atattgctgc tctttctgta agtaaaggta taacaggaag tatatgtaat
    45781 gctaagacta tatttgaaga agtagaacca agattaagcc aaagtgaagt aaaagagtgt
    45841 ttgaaatcag gtacactaat acttgatttt gatgatggag acgtaattat agttgatgat
    45901 gtaaacacat ttaagaagta tgtagatgat aagaatgaag ctataggata tatctctaat
    45961 attatgttta ttaatactat aaataaagat acttcattga agcgaaagga atttgtaggt
    46021 aagatattta atgattcaac agggcagaca actgttatat gtgcattaaa gaaatatttt
    46081 gaagaattaa tgggtcaagg cattatatca gaatttaatg ttgatataga tacagagctt
    46141 caagcaactg ctaaagcaga tgagttttat tggaaatggg atgctgtcaa ggttgatgtg
    46201 atgaagaaaa tctttggaac tggatattta ggataaagga ggttataaag tatgggaaaa
    46261 tatgatgaaa atattataga tgctgcaaat gttgcagatg gctcgaatgc taaaataata
    46321 gttgatggga aagaagaagg atatggaaca gagtttacag ctgaggttga aaatgataag
    46381 aaaaccttta gagtgattgg ttgcaaatgg gagcttaaca aggcatctac tcaaaaaggt
    46441 actttttctt taactgtact taaaacaaca tctgaatgga tcgaaagagg atttgataaa
    46501 tttgaattaa tatctgaaat agaaaatcct ggattagttg gatatgaaag aattagatat
    46561 aaaaattgta tggtagataa aatacaacta gcaagtataa aagctgatga aaatatagaa
    46621 atacaaatag atggtacttt tgagggttat gagctagtgg ataaaatagc ataataaata
    46681 aatttaagct acatgtaatt aattttatat gtagcttttc aaataaaact ataaaaatgg
    46741 aggaagttaa aaatggcaaa cttagataaa gaatttttaa atgaaggaat agaagaagaa
    46801 agagaactta ctaaagagga aatagcaaag caacaagaag ataatataat tatgaaattg
    46861 acagaggatg ctatattacc tgaaaaaact atttttgtaa aaagattaga tataccactt
    46921 acacttaggg ctttaacaga aaaagagata agtgcgttgc aaaaaaaata tacaaaagtt
    46981 actaaggtaa gaggtagaag ggaaagcaaa ctaatggaag atgaatttaa tatagctcta
    47041 atagaaaaag ctacaatagt tcctaatttt tgcgatgcaa gacttcttaa ttctatgaat
    47101 gtatctagtg gtgttgaatt tataagaaga aagttcttag caggagaaat cgcattaatt
    47161 agtgatgagg tactagaatt gtctggattt tatgaagaat taagtgatga tgatataaaa
    47221 aactaataaa gagaggtggg aagattacta ttttatacaa cgcatatgtt aaacatagta
    47281 ttcttccaga ggacttttta aaaagagaga aaacacctca acagcttctt agagttttta
    47341 ctcaacatga aatagaacaa gaaaataaag ctatgaaaaa taaataaaat ttaaactaaa
    47401 agtgaggtga gagaaatagc taaaaaagaa atgtatcaca ttgatgttgt cattgatgtt
    47461 acaggagatg aacaaactaa aaataaatta agtgctatgg aaagatacac gaaacagaca
    47521 gaaaagagaa tgagagcact aaataggata aaagctaatc cagttataca agctcaagat
    47581 aaaacatcta gtgttgtaaa tagaattagc aacaacttaa aaagagtggg tagaactata
    47641 tctacaacca taaacgcaaa agatagagca tctagcgttg taaatagagt taaaaacaaa
    47701 gtaaatagct tacttacaag tcgacaaaga gaagttttat taaaggctag agacaaagct
    47761 agtcaagttg tagataaagt aaaagctaag gtacaaaatt tgactgcggc tacaataatt
    47821 agcttgaata tgaaagctga cccagcacta agagttattt ctcaaactag aagtaagtta
    47881 ggagaactca aaaacaacac aataataaat attaaagcaa aaggtgaaga agcattaaat
    47941 actatttctc gtactaagag taaattacaa gagttttcta ataggactta tcaagcaatt
    48001 gtaaaattaa aagatgaagc tagtccaact ttgagtgggt tagatggtaa gataagttct
    48061 tttataagta gtactattag taagtttaca caattagcag taacagccac agcattaatt
    48121 ggtggtgttg gagtaggaag tgctataaaa ggatttgcag actttgaaca agcaatgaaa
    48181 aacgcacaag ctgtatcaag tgcaaattca aaggaaatgg cagaaatgac tgcaatggca
    48241 agggaaatgg gtcgtacaac tagctttaca gctaaagatg cagggaatgc tatgtatttt
    48301 atggggatgg ctggatggaa aagcaaagaa atgattgctg gtctacctgg tatattaaat
    48361 ttggcagcaa caggtcagac agatttagca ctaacagcag atattgtgac tgatggttta
    48421 actgctttag ggctaactgc aaaggataca ggaatgtttg ttgatgttat ggcagcaaca
    48481 gttacaaact ctaacacaga catagaaaga atgggtgaaa cttttaagta tatgggaagt
    48541 gttggaggag cattaggtgt ttctatgaaa gatttgagtt tagcaactgg cttaatggct
    48601 agtgcaagcg ttaaaggtag catggcaggt actgcactta gaggtggttt agttagatta
    48661 ataaaacccc cagctgaggc acagaaagct atgaataaat atggaataga aataaagaaa
    48721 acaaaagatg gaaatttaga tttagctagt acaatcgttg accttagaga aaaattaggc
    48781 ggacttgaag gagtacaaaa aagtgctgca ataagtagca tatttgggcg aacagccatg
    48841 gccggttggg cggctgtagt aaacgcaagt gaaaaagatt ttaaaaaatt aacaacagct
    48901 ataaatgaaa gtgaaggaga agctaagaga attgctgata tgaaactaga taccctgtca
    48961 ggacaatttg aaattttaaa aagtgctatt gatgatgtaa gaatatcagt aggtcaaaga
    49021 cttggaccta tgacacgaag ttttgttgaa cagttaacaa aagatatgcc taaaataggg
    49081 gatgccatcg ttagttttgt aagtgatttt ataaatgatt ttgataaaat taaaagtatt
    49141 atgcaaaatg tcatttcagt aatatctgga atagtatctg catttattgc tttcaaagct
    49201 ttaaagttta tttcttttct aattcctata ttaagcgata taggatttgc aatagctgct
    49261 tttgcaggtg gtgcggcaac gctaggagaa gctttattgt ttgcactagg tggacctgtt
    49321 ggtgctgtta tagcaggagt agtattacta gcaacagcat ttacattagc ttatcaaaaa
    49381 tcagatgctt ttagaaaaat tgtcaaaaat gtaggaaaat caattaaaaa ttttttacaa
    49441 gaagcaataa tagcaatttc accttttgta aatgtgtttg gtaacaaatt aaaagaatta
    49501 gggaaagctg taattccatt gttaaaagta tttggggatt ttgcatcaac gctaatgagt
    49561 aaaatcggac ctataatttt gtttttatca agtaatgttt tagctggctt tatattaact
    49621 tttgtagctg ctgtagaagc tgttaaatct gctgtagtcg cgataacagg tgtattgcaa
    49681 ggtttaacat caattataaa aggagttttt gatattgttg gaggaataat aagtggtgat
    49741 gggaaacaaa taataaatgg cttaaaatct gtatttgaag gaggaataaa aattgtttct
    49801 tctgtttgga aaggattagt agatattgta acttctccta ttcaagctgt tgtagatgtt
    49861 ctagacgaaa aattcggaaa aaaagtagag ggaataaaga aaaaatggaa tgaattaaaa
    49921 gacttcttaa aaaatcctac taaagcagct ccaaaagttc aacctgtcaa tttatctgga
    49981 gagaaagcat caaacgagtt acaagcttca tcaaacggag caaaagcata tataagttcg
    50041 ttaggtcaaa aaataggtga aggtattgga aaaattaaag ataaatttgg agaactcaaa
    50101 atatctgcaa cagaagtatt taataatata gtatctttcg taggaggcaa agcaaccgaa
    50161 ttaaaagata aacttttaga aggcataaaa cctgctatag atacatttaa acaatcatta
    50221 agtaatttaa aagaagtttt tggagattct ttagatagca taaaagaagc ttttggaggt
    50281 ttaaaaactg tatttgatga aaatattaaa acgcctttcg aaaatttaaa acaaaaagtt
    50341 ttagaaacaa aagaaagttt aaaaccagtt tttgataact taaaatctag ctttgcagaa
    50401 ctaggaaaag ctcttgaacc aataaaagaa gcatttagtg gaataaaaga tttcttttca
    50461 aatttgttta agccaattaa agatgatgga gcaactaaga caactaaaac caatatggat
    50521 gagttaaaac aatcaacaca acgtgttgga acatctttca aagagttagg aaatgctttc
    50581 aaccaattaa aagaagcagc aaaacctttt atagactatt taaaacagat aaaagattct
    50641 ttaacatcta ctctgggaga tataggggga ggattactca aaggtgtagc aacttctata
    50701 gttttagtta taacttctgt tattaatgca attgcatcta ttataaatgc tgtagcaggt
    50761 gctataaaag gtgtaattga tataataaaa ggaatattcg aaatcatagg tgggataatt
    50821 agtggtgatg gtgaaaaaat aaaacaagga ttttctgatg ttttcaaagg aattggggaa
    50881 gtagtcaaat cattgtggga aggtataaaa ggagttttag gagcaccact taaagcagtt
    50941 gtaaatttta ttgataatgg attttcagaa aaagtagggc aagtaaaaca atggtggtct
    51001 gatttaaaaa ctaatgtagg ccaaaaaata agcggatttg ttagttttgt aagcaatggt
    51061 tttcaacaaa aagttcaaca agttggaatg tggtggcaag gacttaaagt aaatttatct
    51121 ggcaaaataa gtggatttgt aagtcttgta gaaaacggat ttaaaagtaa agtggattca
    51181 attaaatcag catgggatag tttaagaaaa aaattatcac aaaaaataac tggttttgta
    51241 agtatagtaa aaactggaat aagtaatgta ttggaccgtt ttgctgatgg tggagttgca
    51301 agtaaaccaa gtatttgtgg agaagcaggt cctgaaatgg ttattcctct ttctaatagt
    51361 aagagaagta gagcattaag tttatatgaa caagcaggac agatgcttgg aactaaagca
    51421 agcaataatg ttattccaat atctcaaaaa ttaggaacta gttttaattc tgcaagtagt
    51481 atccaaaata gtaattctag tattattaat aatgttagac aatttcctac caaacaagaa
    51541 gaatttaata atacagaaaa tagaatttat caagaagctc aaccacaaaa cataatttct
    51601 agtggaagta atgcgattaa tgttggtgga atatctataa atattcaagg tagcaacaac
    51661 aaagaagaaa tgatacaaga aatattgtct caagtggaaa gtggattaag agaagcatta
    51721 gaagacattg gataatgtcg aattgttgtt aaaaaaatcc tccttataga tgttataatt
    51781 taattataaa ttacatgagg gggatttgca tattatgtgg ggaagattta aaaatatgag
    51841 tataatctta aaggttttag ttttagtttg tgctatagca atttttccaa taactttatt
    51901 agcattttct atagaatttg tagttaaatc ttttaaaaga aacgaaaggt ttaaagttgt
    51961 ttttggcgta tttttagttt ttattacatt ttcatttgta tgtatttggt atttgttaga
    52021 agataatata acaactagca atagtagtaa taaaaaacaa gaagaacatc taaaagaaga
    52081 gccaggtaaa aatctagcaa caagagaagc agaagaagaa acaaataaga agaaagaaca
    52141 agagaaacag aaaaatgaag gtacagaagc tgaaaaaaaa gagcgagata aaactgagaa
    52201 aaaagaaaaa gtagataaaa aatatcaaga gaagaataaa gagaaagcag aaacgaaaaa
    52261 gcaaacatta actagtgaag aattaaagaa aaaagttgat tctataattc cagcagagta
    52321 taaaggaaat tattacacaa atgatgtatt agatacagat ggtagctatg tacttagttt
    52381 acaagttcaa aatgcaagtt ttgacaatga aagcagttgt aaggcattta ctaaagactt
    52441 aattaacaag ttgaaagaat ttagaataga ctcagcagaa gtatattttg taggttcaag
    52501 tggtcaaaca acatatcaaa ttaatataga tgatttcttg aaagttcaag ataatattaa
    52561 gagtgtagat aatatggagt ttttctcttt caaagatttt aaaaattaga tatataagaa
    52621 aaagacgttt atttattagg aatagatgtc ttttttattg taaaaaggaa gtgataattt
    52681 ggtaatagac atatacctaa aaaatgaaaa agaaaaaata gattttcatt ttccagtaaa
    52741 tccattcgat agtttatcta ttaaaaaaga aaaaaggttt gaaactgtag atatagtaaa
    52801 cttaggcgaa tttgacatta aaaaagaagg agagaagata agagaaatat catttaaaac
    52861 atttctgcct aacttatatg acgcttctta ttgcagatac agtgagttaa aaaatccaat
    52921 cgaagtagtg gcaatgcttg aaaagtgggt agaccaagct gaaccattac gattaatagt
    52981 aactggtttt ggctacaatg gattagttac aatatctaat tttagtaata ctcaaacagc
    53041 aggaagagaa gaagacagag atattgagat aacatttaga acttacagag aactgaagat
    53101 agagacatta aaaaaagata caaaaagtaa tactaaaaca gatttaaaag ataatagacc
    53161 taatacccaa actaaatcta aaatatatac tgttaaagca agtgatacat tatataagat
    53221 agctaaaaat cttttaggta agggttcaag gtggccagag atttataata tacccgaaaa
    53281 caaaaaagtc attggtaaaa atcctaatat aattaaaaaa ggtcaaaagt tggtgatacc
    53341 tagtaaatga aaataatatt aaacgggaaa tatgatattg caaatttcaa tgaaggaata
    53401 actctttcag aagctataga cggagttgca tataagatgg atgtatcatt agtagaacct
    53461 aaacaactta aagatataaa tattaaaaaa ggtgataaaa taattctaat tgatatagca
    53521 tatgagagta aaaaagaaga gacaatattt gatggagttg tatgggaaac tagaaggagt
    53581 gaaaagagta agaaactaac attgtcttgc agagaaagaa cagtttacat ggaagaatca
    53641 gaggaacaat atcagtttaa agaaaataca gcaacacaga gaattgaata ttactgtaag
    53701 caatggaata taccctatta caacttagct aatacaggga agaaacttgc taaagtaata
    53761 cataagacaa atatactaga tatgataaaa aaggacttaa aagaaacagc aacaaaaggt
    53821 ggagacttat ttagagtaag gatggataat aaattaaaat tattcaagct tggtactaat
    53881 gcaaatgtat ataaattaga tagtatatta gaagatgcta actttacaag tagttttaat
    53941 gatgcagtaa caagtgtaaa agttttaggt aagagtaaag acgaaaatac aaaagcgcct
    54001 ataattggga catataaaaa agatgctgat aagtttggaa cactacaaaa gattaaacaa
    54061 gatgaaaaga taaaaaatgc taaagaagct aagaaagcag cagaagcaat gttcaatagt
    54121 ggagaggaaa caataagtgt agattgtgca gtagatataa atagaataag agcaggtgac
    54181 aaagtaagtt taaaaagtaa agaatattat gttatagatg tcactcatac actagattct
    54241 agaccgaaaa tgaagctgaa tatagggtct ttagaatata taaggaggaa attttataat
    54301 aatgactgat gctagattta atggaattgc tagaatattg aaagaaaata tgaataaaag
    54361 tgtagcaaat ggcacttttg gaatgggttg tgaacttgca gaaataacag caaatggatt
    54421 aaaagtaaat ggctataaag atgaaataca ggactatcta gtattagaga atttaacatt
    54481 aaaagaagat tattttactt tttcagatga agctttaagt ggagaatata gacataagca
    54541 taaaatagaa actccaaagg aattgaagcc actacgtata ggcgataatg tgctagtagc
    54601 tgttatggga gctgaatttg tagtaattgg gagggttgta aatgccaaac ctatttcctc
    54661 aaagtgaaac ttttgaaact gtagaattaa aaaataatga tgaaaatgaa ttggacctaa
    54721 agggttcttt tttatttgat tttgaaaaag gtgaatttgt taaaaacgca gatggaacac
    54781 taaaaaaatg tgataaggtg caggcgtaca aacaatggtg tcaaaaggct atattaacac
    54841 ctagatacaa aaaagcagct tatacaaaca tttatggaag tgaaataaaa gacttaatag
    54901 ctagtaactt atctcaaagt gcaaaagagc ttgaaataac tagattaata aaagaaacta
    54961 ttttggttca tccatataca aaagaagtta gtaattttat atttgtttgg cttgaaaata
    55021 gtagacttgt taattatgaa tttgatgtgt taacaataga tgatgaaaat ataaccatag
    55081 atggaaacat aaaaaggtag gtgattatat ggaaagagag ctacctatac cattattttt
    55141 aacagaagag gaggactctg tacatgaaag gatgttaagt aactttcaag atgtgagcac
    55201 actagaaggg gactttattt atgatgcaac aagacctaca gcagagcaga tagctgaatt
    55261 aaaacaacta ggattacaaa ataatttaag gattgcattt cctcaaacta gctatggaga
    55321 atatctagaa tggttgggag aatgcaaagg agtatttaaa aatcaaccaa ctaaatcggt
    55381 tggtatggtt acatttaatg gtgctcaagg aactatcatt acaaaaggaa ctattgttac
    55441 aactatagct acagatgaaa aacagagcat agaatttgag cttcttgaaa ctaaaactat
    55501 aggagcaaat gaaacagtag atattaaagc agaatgtagg attgcaggaa ctataggaaa
    55561 tgtatctaat aacactataa ctgttttact aggttctatt agtggtgtta aatcagtttc
    55621 taataaagaa gatttcagag gtggaacaga tatagaagat gaagaacatt ttagagaaag
    55681 agttcttgta gcagagcaag aggacaaatt aagtggagct agttcagact atataagatg
    55741 ggctaaagaa gtagatggag tgggatatgc ttatgtagtt cccgaatgga atggagcagg
    55801 gacagtaaaa gtattaatac tagataaaaa cagaaaagca gcaacacaag aattaataga
    55861 taaggtccaa gaatatatat atccattgaa tatatcagaa ggagaaaata gagatgggaa
    55921 agctcctatc ggtgcattag ttacagttgt gacacctgac acattactta ttaatgtaaa
    55981 agctagtttt atatttagta atggctttag tgaagaaact gtattaaaca atctaaaaac
    56041 taagatagat aaatatttag ataagattga tttagggggg acagtctcat acaatgctat
    56101 acaggcgata gtaggctcta tgatgctgac agatgaaggt atagaagact tttctaatct
    56161 tactataaat gatgtaaaag aaaatataaa attgcaagac caagtggtcg gaatagggga
    56221 aatagttaac gaggtggttg gatgatagct tctaaaaaag gtaaagaaat gcttcttaca
    56281 ttatctccta tctatgaaca atctatcata atgcaaagct tatatgaagc tataggaagc
    56341 gaatttgata atctagaatt attaaataaa gaaatagagt tacaattatt ccctcagagt
    56401 gcgacatggg gacttgaatt ttgggaaaat agagtgggtt tatccactaa tatagatgaa
    56461 gatatagaag ctagaagaag aaaggtcatt gctaagcttc aaagtaaata tattatgaca
    56521 cctaaaagaa tggctatgat actccaatct tatacaggtg caaacataaa aataaatgaa
    56581 aatatatctc catatacttt tggtgttgaa ttaaccagta cccaaggttt tcctaaagat
    56641 ttagaagatt tatataagag agtaaatgtt ataaaacctt ctcatttagc tgtaagttat
    56701 aagttagttt ctttattgaa aagtaaaacc tattttgcac aaacggcaat tatgagcgaa
    56761 gaaataactg tatatccgta cacaagcaaa gaagtaaaag caagtgttaa agccaagttt
    56821 gcactagctc ataacatgag ctcagaaaca ttaacagtat atccaagata ggaggtggca
    56881 taaatggctg atgaacaatt ttacacaata ctaacaaaca taggtaaagc taagattgct
    56941 aatgcaggaa tgttaggtaa gtcagtaatt ttagagaaga ttcaagcagg tgatggtgga
    57001 ggaaactact ataatccaac agaagaccaa acagcattaa aaaataaagt ttgggaaggg
    57061 aatataaatg cttttgataa tgatgaaaat aatcctaact ggattattgc aacagcatgt
    57121 atccctggtt cgataggtgg atttacagtt agagaaatgg gtcttataga taatgaagga
    57181 gatatgattg cagtttgtaa aagccctgaa acctacaagc caaaagttga taatggagct
    57241 atgaaagatt tgtatttgaa atttatcata gaagtatcta atgtagagaa agtgacatta
    57301 gttgttgacc ctacagctat ttttctaact aaaaaagatg aagaaaaaat attaacaaat
    57361 attaataaac tagacactaa aatagataca accaaaacag aattaacaag caacatagaa
    57421 actactaaaa cagagctaaa cactagaatt gacacagaaa atgagaaaca aaatattaaa
    57481 attgaccaat taatcgcagg tggttcaaat gtggcatcta ctcaaataat aacaattgat
    57541 gattgggttg aggatgcaga aaatggattc aaagcaactg taacacatag tttattaaca
    57601 cagagaatag ttgtaaatat tatagatgct actacaaaag aaaatgcagt tacaaacttt
    57661 aaaattatag atgataattc tatagaagtt agaagtgaaa caaggtcaga attaaacgtt
    57721 tatgtgataa atggaaatgc agaaactcat tttattaatg caactgtaga tgataacaga
    57781 gtgtctgaaa tgactactta ttcatctaag aaaatagagg attctattag cagtatacag
    57841 cttatagata ccagtataag tattacagat gctaatgata gatttacaag tgataagtta
    57901 gatggagtat tagaggaaat aatggtagaa ataagtggtc aaagaactaa aggaataact
    57961 atagtgaata atttaataga tatgatataa gcgaggtgaa aatatgacag aaaaattaac
    58021 tgaaaatgct agtttagggg aacttatggc agcattagaa aatgtacaaa ctgattttca
    58081 aactggtaaa aataatatat ctagtgcatt gggtagtcct tttattggaa cagataaatt
    58141 tggtacaact aaaacaaaaa tagaaacatt aaaaaatgta ttagtggaga cgattaattc
    58201 taaaaatgtt tcagcaacat catctgaaac atttactaat ttgattgaaa aagttaactg
    58261 gatttttcaa tcaatagaaa ttttttcttt aaaaaataga attcaagcta caactttaaa
    58321 cactcctagc atcgtttaca atgaagtatc tagtataaaa ggcacattaa gattcacagg
    58381 cgaacttaaa gcgtctaaaa tgcgtgctga ttatgcaaca gcaaaaatag agatactatg
    58441 tggaaaccga aaagaatact tttatgttac tgacgatact cctagtgcat cttcttcttt
    58501 tgtaagattt acaaaagata ttattgttga gaatggcatg gatataaaag tacaaatatt
    58561 attaacatct gtaggagcag gaaatttaga tggttcatat gcagctcgtg cagaaataga
    58621 aaaattaaca atattaaggt aggaataaat tatgaaaaaa caagtatatt ataattcttt
    58681 agatgaaaaa gaaaaaataa ttagtgaaaa ctctaattta tatgttatag aaatatatga
    58741 aactttaaat gaaaattatt tagtgttatc aagtagtcca atacaagatg aaaaacttag
    58801 ttatgaagaa ttagaaaatg aattattaat tatgactaat gaattacaag gaggattgtt
    58861 ataatgaata taaataatgt tgtggtaaga atattagcag aaagaatatt aaatggaggg
    58921 ttaaaccctt tgaaaaatag agagtttcaa cttgatgatg taactaacac agaatacaga
    58981 aaagcagtag aggattatat tataaaaaat agtggagtag tagaaggaat agaaccaaca
    59041 gcgtagtagg ttcttttttt attgaaagaa ggtgactaaa tgacttttaa agagttagtt
    59101 aataaagtta gaaatcttgt attagaagca aagaatgtaa ctatagaaga tacagagaat
    59161 aactttacaa gtgataatgt agaaggagca ttaaaagagg tttttcaaaa tggagttaat
    59221 gctaaaaata atgtagtaac agcattaaac tccaagggag cagaggttac tacaagcgat
    59281 acatgggaag aaataaagaa taaaattgat ataaaagagg ggagattaga tttaagagaa
    59341 acaatacttg caaatagtta ttcttcatat ttggttacaa atggtgctat aaaatatatt
    59401 gaaaaatata gtgggagttt aaaagcatta gaatatgaag aaccctattt ttatgcaatt
    59461 aaaggtacac atttaattaa aattaatgca attgatgaat ctgtaatttt tgatattact
    59521 ttagccaatg ctaacttctc atgtatctgt gttactcaag agtatttatt tatatctgac
    59581 aatactaaat tatataaaat aaataagtta acaggaaatg aagcacagtc aatagaaggt
    59641 tcttattata agctatgtac ttatggggaa tttatttatg gaatatatgg agatgagact
    59701 tcttcaatac ttcacaaaat aagaatatcg gatatgtata taatgctaac taaagatatg
    59761 tcctctgata gaatttatga ctttgaaaga ggcaaatttg tatgtaataa taatggtatt
    59821 tatgctacaa cagaacactc aaattcaagt ggtcttacag aatgttacct aactaaaata
    59881 aattttgatt ttagtgttgc gaaaagtttt agaattggag gatatttata tgaaaaaaac
    59941 attaagtttt taaatgattt tgtttttgta tctgatagta gcagaagcat agaagttgag
    60001 agtggtaaaa aaagtggatt agcaaaatat gatgcaaatc taaatttgat tacttatggt
    60061 gggtcagata gatatgaaaa ttttgaaata tataatggat atatatatac atttaattca
    60121 ctttctagta gcccatttat aaaaataaat ttaaatactc ttaaagaagt taatagctac
    60181 cgaacgctca ttaacacaaa tcctcaaaaa ggtatgttta taataaacaa tataattttt
    60241 tttattggtg gtggcattca cagaaatata ttatcaaaaa aggtttattc agatgaaaaa
    60301 ggagaggaat tatgatttat ttaggaaatt ttatggagac agatgaacaa aatataaaat
    60361 atattgggat gatacactat aaaccaactt tactatcaga agaagagtta aaaaatggaa
    60421 ttttaattga gaaattgcca gtacaacaat atgtagaaaa taaagaagca aagttattta
    60481 taaatataga tactaaagag gttttctata gatatacaga tattaaaagt agcatagaag
    60541 ataaagtaaa ttctacagaa caaacaatag cagatttaac atttcaatta atgtcaaatg
    60601 gggtgatata aatgaattgg tacaagataa taacagattt ctataataat ggtaattgga
    60661 ctaaagagca agttaaaacg gcagtagaaa agaataagat aacggcaaca gaatataaag
    60721 aaattgtagg agaggactat atagcatagt cttttttatt tcttaaaata tataaattca
    60781 tttacatttt gcctacactt catctacact ttgtagacgt ttgtagtttt aaaattaggt
    60841 acaataaaac cataaattaa gaaagaggtg gattaagtgg caacagttta tgaatttaat
    60901 tatacgggaa gtgaacaaag tgttacattg aaaccaggta aatacaaatt tgaatgttgg
    60961 ggtgctaggg gtggtgcatt aggaactcct ttcgaaagtg gtttttatta tggttatggt
    61021 ggatattgta gtggtgaaat aacattaaaa aaagaaacta ctttatatct ttatgttgga
    61081 atagatggta gaaaaggtta taatttcaat agtgctggat atggtaatgg tgcaagtggt
    61141 ggtggtgcta ccgatattag gcttattggt ggtacttggg ataatgaaca aggtttacta
    61201 tctcgtataa ttgttgcagg tggtggcggt ggaacttatg ataaacaaca tggtggtgat
    61261 ggtggtggct tgaaagggac tcttggaact tcttctactg gtgctgctgc tcatggtggt
    61321 acacaatttg agggtgggcg aggtagagac aaagatggtt cttgtgatgg tttttttggt
    61381 aaaggtgcta ctcctaaaaa cccttcatca caatctggtg gtggcggtgg ttggtttggt
    61441 ggtgcttatc ctgctagtgg atttggtaat ggaagtggcg gtggaagtgg ttatgtacta
    61501 actaaagata gttataagcc acctggatat acaccaactt ctgaatatta ttttgataat
    61561 gtagtcatga caactggtgg aaatactact gttgtaggca attactcaga tggtcgtgct
    61621 aaaataacat tattacaagc attaccattt ttgaatatat cctcatacaa ctcaactaca
    61681 gcaacattca aagcagacca cacagaccct acactactaa caaaaataga aatatttata
    61741 gatgatatat taaaagaaac tattacaaca gatttaattc ttgaaaaaac aattaactac
    61801 acattagaag ataatgcact acacactctt aaaatagttg ttacagacag tactaatgct
    61861 acagcagaaa aaatagtaag tctaagtaag aatataatgc cattaggtga tgatgccagt
    61921 ctacaagata tagcaagtaa agttagtgaa ataaaagaag gattaataaa tggcaaaact
    61981 tctatcataa atgtattagc attaaagaat attgaatcaa gtttgaacaa ttctttagta
    62041 gaattgtcag agaaggtaaa agaatccttt gatagttcag atgctagttt gcaggattta
    62101 ataaaccagt taacacaggc taataatact atatcacagt taaactctaa atataaatat
    62161 tctagtggaa cttttactta ttatactggt gcgtatccta atgggctaaa tataggtttt
    62221 aggccagata ttatttcata cactgttatc aatgctgtag aagaaaaata ttattgtgta
    62281 tacacacctt tattctatct tttttataat tatacaactg gagaaaaata tcttgatgtt
    62341 aatcataatc attatggaat aacttttact gacaatggat ggatacctaa aggtccagtt
    62401 aacaatggtg tgtatagtta tatagcagtt aaatgttgtt aattatcaaa ggcttagata
    62461 acttctaagc cttattttaa tataaataag gaggttttca tgaatgaaga acttttcaaa
    62521 gaaaatttga aacgacatga agtaacaata aataaacata atgatgaaat agacgaatta
    62581 aaagtggcaa atatagagtc taaagcagag ttaaaagcat tgtgtgagaa tttaaactca
    62641 cttacaagta tgctcaaatg gctaattgga acaatgatta caacactagt agggttcttt
    62701 atatttgcag ttcagaaagg aatattttaa ttaattagga ggataaaaaa tggataattt
    62761 aataagtttc ataccagagc agttgctaat tttagtagct gctctctcta ttataggtaa
    62821 gggctgtaag aagtataagc aactagataa caaatatatt ccagtagtgt tattaatact
    62881 tggtatagga ttttctattt ggatgttagg actaagtcct gttgcagtct tacaaggcgt
    62941 gatttgttgg ggtatatcaa taggtataaa ccaaacttac aaacagttga aggaggaaaa
    63001 taaataatga aaatatgtat aacagtagga cacagtattt taaaaagtgg agcatgtact
    63061 tctgctgatg gagtagttaa cgagtatcaa tacaacaaat ctcttgcacc agtattagca
    63121 gatacattta gaaaagaagg gcataaggta gatgtaataa tatgcccaga aaagcagttt
    63181 aaaactaaga gtgaggaaaa gtcttataaa atacctagag ttaatgctgg aggatatgac
    63241 ttactcatag aactacattt aaatgcaagt gatgggcaag gaaaaggctc agaggttcta
    63301 tattacagta ataaaggtct agagtatgca actagaatat gtaagaaact aggtacaata
    63361 tttaaaaaca gaggtgctaa attagataaa ggattatata ttttaaatag ttcaaaacct
    63421 actgcaatac taattgaaag tttcttttgt gacaataaag atgattatga gaaggctaag
    63481 aaatttggat atgaaggtat ggctaagtta attgttgaag gtgtattgaa taagaatata
    63541 ggaaatgatg gagttaaact gatgtacaaa catacaatcg tttatgatgg agaagttgac
    63601 aaaatccctg caactgtagt tggctggggt tataatgatg gaaaaatact gatatgtgat
    63661 ataaaagatt atataccagg tcagacagaa aatttatata ttgtgggtgg aggagcatgt
    63721 aataagatag gttctataac caaagaaaaa tacacaatga taaagggtaa tgatagattt
    63781 gatacacttt acaaagcatt agattttatt gatagataaa aggagtatag ttaatgtatt
    63841 ttacattttg cttatacttt atgccaatat tacgacaatt tataaaataa aaaatgatat
    63901 attaaagata atcataagcc attttaaaat ccaaaaaact aggtgtataa acagttaaca
    63961 aatatatata ttgataaaat aatggaggtg aaataaaaaa tagtagtgca ataacaataa
    64021 ttctaatgta gaataatatt aaataatatt attttagggg gatttaaaat ggcttatgaa
    64081 aatggaactg attttataga ttattttcct gattgtatag aaaaatataa ggaagataat
    64141 aaaatctttg agaaggcaat attattactc aatctgtata tgcaaaaacc aacatggcca
    64201 cattgtgtta atgagtacaa gagccaggcg gaaaaaattt taggtgaaga cccagagttt
    64261 ataggaaagt atggtataca attaagaaaa ttttatgatg aagatgaaaa tgcaaaagta
    64321 agtaaggaat ttaaaaatga tttgtataat ttttctaaga gtacatatga tatactagat
    64381 aatgtttatt tttcaaaaag tcatcctttt aatctttcat ctatttattc aacttctaga
    64441 gatgatgatt ttataataaa aatatttaga tatgataata attttcttga attagagatg
    64501 tcaaagagag aagtagagtc attaataaac tatttatctg atttattaaa ggaagaatag
    64561 tatgttagga gcaaaaaaag aaaaagagtt taatttattg agttttaatg aaaaaaagat
    64621 agaaaaacat aaatgtaaaa attatcataa tgattcttcg aaagataaat acaaatatag
    64681 tcaaagtgaa tttatagctg ctactttaaa ggaggcagat gatatgaatg aagaagaaag
    64741 aagaatacta gaagcagaaa aaagaatatt agagactgag aaaagagtgc atcaagataa
    64801 caaggaagta aaagaaagta tagataagca atttaatgac ataaagggta tttttaaaga
    64861 atacaagaat gatttagaca aagatagaat agaactaaaa aatgatttag ctagagaaag
    64921 agctgagtta aaaagtgatt taaaagaagc tataaacgaa cataaaaagg tcacagaaaa
    64981 ggatgtaagt gaaataaaga atagtatgaa aggtatagaa gatagaattg attcaactaa
    65041 taaatggatt attggactat gtataactac tataatagga attgctacaa tggctattac
    65101 aataggtata tctatatggc caaagtaaca caaaaagagg taactagaat taaaatagtt
    65161 atctcttttt atacttaaac aaaacattga aaaaatagta aaaaatatta acaggataca
    65221 taaaactata gtataacctg taataataaa acgaacgaaa attatcatac taaaacataa
    65281 gacatgctat aattgtatta gataaatgct tgaatatata ccaaaagtac tctttttata
    65341 agagtgctta tttttttgaa attcatcaac atataaacaa tcaagaacat tactcaacac
    65401 accttaaaat tgatttaaat tctttttcat acacaaagtt atatgatata ataaaaaagt
    65461 aaatatgtaa ccccaacaca tctttactaa gtcaaacatt attatataga gcattcttca
    65521 ttatggagag tgctttttca tttctttgaa taatcatgtt gattatttat aatattttca
    65581 tttaatttat ctttatctat caaatttaat gcacaattaa t
//
LOCUS       DACULQ010000002        52475 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.002, whole genome shotgun sequence.
ACCESSION   DACULQ010000002 DACULQ010000000
VERSION     DACULQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.002"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(227..1006)
                     /locus_tag="JA154_00435"
     CDS             complement(227..1006)
                     /locus_tag="JA154_00435"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087214.1"
                     /note="catalyzes the formation of L-histidinol from
                     L-histidinol phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HAT7994322.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLILGSDSHCAEEINKYDIV"
     gene            complement(1552..1902)
                     /locus_tag="JA154_00440"
     CDS             complement(1552..1902)
                     /locus_tag="JA154_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HAT7994323.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(1918..2334)
                     /locus_tag="JA154_00445"
     CDS             complement(1918..2334)
                     /locus_tag="JA154_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994324.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(2353..2544)
                     /locus_tag="JA154_00450"
     CDS             complement(2353..2544)
                     /locus_tag="JA154_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAT7994325.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(2695..3510)
                     /locus_tag="JA154_00455"
     CDS             complement(2695..3510)
                     /locus_tag="JA154_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAT7994326.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(3557..4495)
                     /locus_tag="JA154_00460"
     CDS             complement(3557..4495)
                     /locus_tag="JA154_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT7994327.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(4492..5364)
                     /locus_tag="JA154_00465"
     CDS             complement(4492..5364)
                     /locus_tag="JA154_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT7994328.1"
                     /translation="MNKNLSEKMEIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(5389..7317)
                     /locus_tag="JA154_00470"
     CDS             complement(5389..7317)
                     /locus_tag="JA154_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAT7994329.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(7429..7806)
                     /locus_tag="JA154_00475"
     CDS             complement(7429..7806)
                     /locus_tag="JA154_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAT7994330.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(7893..10019)
                     /locus_tag="JA154_00480"
     CDS             complement(7893..10019)
                     /locus_tag="JA154_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087205.1"
                     /note="in acetogenic organisms, this enzyme complex
                     converts carbon dioxide to acetyl-CoA while in
                     methanogenic organisms this enzyme is used to degrade
                     acetyl-CoA to form methane and carbon dioxide; part of an
                     enzyme complex; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HAT7994331.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(10076..10882)
                     /locus_tag="JA154_00485"
     CDS             complement(10076..10882)
                     /locus_tag="JA154_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HAT7994332.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(10932..12299)
                     /locus_tag="JA154_00490"
     CDS             complement(10932..12299)
                     /locus_tag="JA154_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA synthase subunit gamma"
                     /protein_id="HAT7994333.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(12330..13274)
                     /locus_tag="JA154_00495"
     CDS             complement(12330..13274)
                     /locus_tag="JA154_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA synthase subunit delta"
                     /protein_id="HAT7994334.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(13294..14064)
                     /locus_tag="JA154_00500"
     CDS             complement(13294..14064)
                     /locus_tag="JA154_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HAT7994335.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14120..15505)
                     /locus_tag="JA154_00505"
     CDS             complement(14120..15505)
                     /locus_tag="JA154_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAT7994336.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(15549..16430)
                     /locus_tag="JA154_00510"
     CDS             complement(15549..16430)
                     /locus_tag="JA154_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydrofolate reductase"
                     /protein_id="HAT7994337.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(16466..17140)
                     /locus_tag="JA154_00515"
     CDS             complement(16466..17140)
                     /locus_tag="JA154_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydrofolate reductase"
                     /protein_id="HAT7994338.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(17177..18040)
                     /locus_tag="JA154_00520"
     CDS             complement(17177..18040)
                     /locus_tag="JA154_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HAT7994339.1"
                     /translation="MSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVRVGA
                     RSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCFRPL
                     PKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNVDLK
                     GSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRAKMV
                     DESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSILAKH
                     VVKACKLQNNK"
     gene            complement(18141..18773)
                     /locus_tag="JA154_00525"
     CDS             complement(18141..18773)
                     /locus_tag="JA154_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAT7994340.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(18878..20554)
                     /locus_tag="JA154_00530"
     CDS             complement(18878..20554)
                     /locus_tag="JA154_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAT7994341.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(20803..21582)
                     /locus_tag="JA154_00535"
     CDS             complement(20803..21582)
                     /locus_tag="JA154_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HAT7994342.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(21892..23811)
                     /locus_tag="JA154_00540"
     CDS             complement(21892..23811)
                     /locus_tag="JA154_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HAT7994343.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(24418..25329)
                     /locus_tag="JA154_00545"
     CDS             complement(24418..25329)
                     /locus_tag="JA154_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HAT7994344.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(25445..26023)
                     /locus_tag="JA154_00550"
     CDS             complement(25445..26023)
                     /locus_tag="JA154_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAT7994345.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRAIDEIPE"
     gene            26249..27172
                     /locus_tag="JA154_00555"
     CDS             26249..27172
                     /locus_tag="JA154_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT7994346.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQGSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(27103..27330)
                     /locus_tag="JA154_00560"
     CDS             complement(27103..27330)
                     /locus_tag="JA154_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994347.1"
                     /translation="MYLILLVKWGYLKIEISLFFSAEMMNIYFTLYKKECLIQCKVHF
                     ETLSLFIMIYFSLGVSNLDVSNMIEEKSFCI"
     gene            complement(27344..28486)
                     /locus_tag="JA154_00565"
     CDS             complement(27344..28486)
                     /locus_tag="JA154_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994348.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(28504..30258)
                     /locus_tag="JA154_00570"
     CDS             complement(28504..30258)
                     /locus_tag="JA154_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HAT7994349.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            30394..30600
                     /locus_tag="JA154_00575"
     CDS             30394..30600
                     /locus_tag="JA154_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994350.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(30605..31528)
                     /locus_tag="JA154_00580"
     CDS             complement(30605..31528)
                     /locus_tag="JA154_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994351.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(31782..33482)
                     /locus_tag="JA154_00585"
     CDS             complement(31782..33482)
                     /locus_tag="JA154_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAT7994352.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(33892..34305)
                     /locus_tag="JA154_00590"
     CDS             complement(33892..34305)
                     /locus_tag="JA154_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAT7994353.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(34371..36749)
                     /locus_tag="JA154_00595"
     CDS             complement(34371..36749)
                     /locus_tag="JA154_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087185.1"
                     /note="MutS2; MutS-II; involved in blocking homologous and
                     homeologous recombination; has ATPase activity stimulated
                     by recombination intermediates; inhibits DNA strand
                     exchange; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAT7994354.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            37068..38519
                     /locus_tag="JA154_00600"
     CDS             37068..38519
                     /locus_tag="JA154_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HAT7994355.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(38723..41221)
                     /locus_tag="JA154_00605"
     CDS             complement(38723..41221)
                     /locus_tag="JA154_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate kinase"
                     /protein_id="HAT7994356.1"
                     /translation="MNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVAYPIQK
                     GLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENGEIDIM
                     GGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVIENSDT
                     RLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPIEGVRTVAKFSS
                     KPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDEETNYI
                     KETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKSQEDLE
                     NMTKKREIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLDGKRVALA
                     KGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNVRLVPQ
                     TYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLYIMQQN
                     PIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFEYDYKQ
                     DKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQENGTTE
                     MYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQRDSLTN
                     IYNAAYIRKLTSQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDVAKALD
                     ESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLSISMGV
                     VIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(41459..42280)
                     /locus_tag="JA154_00610"
     CDS             complement(41459..42280)
                     /locus_tag="JA154_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductase"
                     /protein_id="HAT7994357.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(42798..43274)
                     /locus_tag="JA154_00615"
     CDS             complement(42798..43274)
                     /locus_tag="JA154_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HAT7994358.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(43309..43887)
                     /locus_tag="JA154_00620"
     CDS             complement(43309..43887)
                     /locus_tag="JA154_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAT7994359.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGNEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(43991..46591)
                     /locus_tag="JA154_00625"
     CDS             complement(43991..46591)
                     /locus_tag="JA154_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAT7994360.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHSNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNVFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
     gene            complement(47161..47844)
                     /locus_tag="JA154_00630"
     CDS             complement(47161..47844)
                     /locus_tag="JA154_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAT7994361.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAVIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(48020..48610)
                     /locus_tag="JA154_00635"
     CDS             complement(48020..48610)
                     /locus_tag="JA154_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAT7994362.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(48635..51028)
                     /locus_tag="JA154_00640"
     CDS             complement(48635..51028)
                     /locus_tag="JA154_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAT7994363.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(51045..52064)
                     /gene="pheS"
                     /locus_tag="JA154_00645"
     CDS             complement(51045..52064)
                     /gene="pheS"
                     /locus_tag="JA154_00645"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087175.2"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAT7994364.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(52114..52348)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT        15913 a        10148 c         5855 g        20559 t
ORIGIN      
        1 acaatcttat ataagaatat ttatataaat aagatagatt aattctaagt cctattattc
       61 tattgaattt tattattatt ttatttagat tctattgtta ttttacttag aacttattat
      121 tattttgttt aagttctatt actattttca atcatataaa tttacaaaag actgtacata
      181 aatttatttt atacaaactt atctacagtc ttttgtgtga atatatctaa acaatatcgt
      241 atttatttat ttcctctgca cagtgagaat cagaacctaa aattaatgga atatcgtatt
      301 tttttaccaa ctctaataag tatccatcta tataaggctc tttgcagtac tcttttctat
      361 ttccagaaac attataatca agttcataac ctttttcttt aattaacttt attaactctt
      421 caagaattgt atttccttta taatcgtatg gaaatacctg attaaacttt ctaactaaat
      481 ttaaatgacc tattcttttt ggtttatgta tacctaaatc tgaatttaca gcttttatca
      541 atgtttcata atacttatta taaacagctt caatacttcc taatttgtcg attaaatttt
      601 gaaactcctc tacactgtaa tctacacaat aataatcatc atcaatctta attatatgta
      661 ctgataaaag agaatcctct aattcctttc catatttatt taaattctcc tttatccctt
      721 cttcgtatcc ttctataaaa tccacttccg acccaacatt tatcttgatt ttaccctcat
      781 acttttcttt caacttcttg acatcattaa aatactttgg taaatcttca atactcatac
      841 aactatcatt ttcaggagca gggtctctaa atccatttgc atacggaaaa tgttctgtaa
      901 atgtcatttc tgttattcca acatccattg ctctttgtac atatttttca aaatcatcct
      961 tacttccatg aggacaataa ggtgaatgta tatgaccatc tttcataaat aattcactcc
     1021 ttttttaaag aaaaaatcta atataaatgc ataaatcttt taacttcaaa ataatatttt
     1081 taatcatgct aatttttatt atatctaata ttagagtaat tttaaactgc cttttaagta
     1141 tcttatcatt agtactattt tcaacttaac attaatatta ttttttaact taacattaat
     1201 actatcttta aatagtaata ctcaatatat aattacttaa cataaaataa ctcaaattgc
     1261 ttgatgaggc ttttgcagta tttaaagaat atataattat ttaacattaa ataatccaat
     1321 cttgtaataa aatttactca ttaaagtata ttacagaaaa tacctcaaaa taaacttttt
     1381 agttcatgat atactctttt ttgcatgaaa ttagatatat ttttatcatt tcaacaatga
     1441 aagggaggct tatatccatc tttagacaac cccttagata aaatttttaa taattaaaca
     1501 tcacaaaata taaaaatcgc ttatatcaaa cgtataagcg attaattata tttacatatg
     1561 ttttttagcc tctaacaaca ttttattagc ttcttccata tactctatag ccttttcaat
     1621 actctttgct gtttcttctt ttccaatagc tctagccttt tcaacccact ctctaaatcc
     1681 ttcttcatga gtttcattgt gatttatcca atgtactaaa agtattttta aagttttttc
     1741 atccttagac tctgatgatt cctcatgact atgttcgtgg tcgtggtcgt gagcatggtc
     1801 atgtggatgt tcatgacata tatctccatg agtatgttcg tggcagtggt catggtcatg
     1861 atgtccatga tgatatctta caatctcttt atcaaaaatc attagttatt ccccctgtta
     1921 ttttatatta aactcttttt ttataaggtt tacagaatct tttacaagta gtgctaatgg
     1981 tttaccttct aatccaacta actccacatt ttctggcata ataggtaaaa gtatcttaag
     2041 tgcctcactg tctgcaatag cttcacttat attacaagta acttctccca tcatagaatt
     2101 aggtattatt acagaaatag gtgcaactat tatatttgct tttttagctg atactactat
     2161 agcattttcc cctgtagctc ctttattagc atgagctttc atcatagaac tagttgctat
     2221 agaattagtc cctagtgcat aaatttctac atatgtaggt aactcttctc tcaaagaaga
     2281 aactatctgg gctccaatac ctccacccat tccatctatc actgctatta tcatatgaaa
     2341 ttcccctatt aattaatttt cttgtattat tatcttatgg tccatcaatc ttatttcttt
     2401 aattatccca tctattatct tttgctctcc taataaatcc gttaaataaa tctttccatc
     2461 acaagggtct atattaacca cattttccat aaccttttct aactcattat tacttttaca
     2521 aatgaaagct gatgattcac acatataaat ctcctcctaa tatgattttt attaaaaatc
     2581 aaaaaaagat tcccttaggt atttcaatac accaaagaaa ccttcatgat ttcagttttt
     2641 gtatattatt ataccttaag tatatcactt taatttatgt aaagtcaatt aaagttaacg
     2701 ctttttatta tactcttcat tcttgtattt ttcatatagt tctttaacta attgcttctc
     2761 ataatcagtc aaatcgccct ccacaaactc aacattaaat tgttcagcta atccctttac
     2821 tatgtttttc tgtagaatat ctatgtctat ttttctgcca atttcatttt ctattccact
     2881 tgctttttta gctgtaaatt tcatagctct ttctttcaat tctggtgtct tagtttttat
     2941 taatttaaac aatttttcta catcaaaatc cagtattatt gaaccatgtt gtaataaaac
     3001 accatctttt ctgctttgag cacttccaat taccttctta ttattaatag taacttcata
     3061 tgatgcatgt gcattaaaac atgcagctga taaattttcc ctacttattc tctcgcctct
     3121 atttaaatta tctgtttcta taccacttag attcaaccct ttaactagac cttcacttat
     3181 atatctataa gataggttga tgctcttatc gattaaagga ttatcttctc ctataattat
     3241 actataagtt agctcattat catgtaatac agctcttcca cctgtaattc ttctagtata
     3301 gtctatattc atacatctac acttatctaa atctatctca tcttccaatt tctgaaaata
     3361 tcctatactc aaacaagctg gttcccatgt ataaaatcta agagttggct tattatgtcc
     3421 cttcttatat gcagtaaata tagcttcgtc aattgccata ttcattgctc cttcatatga
     3481 tttattatgt ataactctcc actgattcat gattattacg cccccatttt tagtataaat
     3541 atatcctaga aatcctctat agatttattt ctcttattgc taaatctatc tcactttgat
     3601 ttaaaggtct tgaatagttg tatatagtac ttcctggtct ttcattatag tatggtctat
     3661 tacaatcctt acaacctctt atttcaaaag gatagccttt gtttatgtct tttaaaatat
     3721 cattgtcaag ctttatacta tttaagtatc catttttaaa cttaaaatac tcttttggat
     3781 aacctttatt tatcatataa gacattaact gtacccttct ataactttct atgcttggtt
     3841 gatttatctt ttccatttta gttcctctaa ctggagtaaa tgcaaataaa ctaatcgtta
     3901 catcattttc ctttaaataa gtatataaat tgtatatatc ttcatgactt tcacccatac
     3961 caactattat atgtgtactt attttattgg gatatgattt tgacatttca gttataaatt
     4021 ttaacttttc atcatagtta tttcctttta ttttttcata tagttcttta tttgctgcat
     4081 ctattgctat tcctatttta tcaactccta ctgaaaaaaa cttctttatc tgttcatcat
     4141 tttctaattt tgcagatagt gaaattggca tgtctatctt accacttata tagtttataa
     4201 agtctaaaac agagttatgt gcttcttcac ttgccattga ttgaatacat attctcttta
     4261 tattttttcc tttataagct tttaaagcat ctaatatttc ttcttttgaa aactctggcc
     4321 aaattactct tgataattta tcttttctag tactactttc tattgattga gaacaaaaac
     4381 tgcatttatt tatgcatttt tctccaatca ttatatatgc tgtggtagga ggtatatcgc
     4441 tttttttgtt taatatccca agttctattg ctgtcccaac tgatagtcta atcataaaac
     4501 gcaacactcc ttacactcat ttattgtcat attcatttct atagccttat tctttgcaga
     4561 ccttgatggt aatactattc tattgactcc acacattata gataattggt ctagctcttt
     4621 tctatacact cctcttggtc tcatacaacc tagattaatt tcagtatctg gtaaattaat
     4681 tctagcttca cataaaatat ctgctacccc ttctaactca ggaggttcta cattttcata
     4741 ctcagtccct tttgttggaa taaggactat aaatgtaagc ttattaggag gatttttttg
     4801 taggtattct ataatttcat attcaccctt tatttgtcct ccttttaaac caatacatat
     4861 atgtggtgct acttctgtat gttcttgtat tgtattatag acttcaatat aatcttcttt
     4921 actctttttc atcttataaa cttctcttat tgtctcatca tcaaacacta aatcaaaaga
     4981 aactatatct aaatacttac acaactctac aatgctatct ttatccataa gacctaaatg
     5041 agcattcaat ctatatcctt gctcttttaa gtttttaatg gtattaatat gagtatttat
     5101 agggacatct ccctcataac tacaaccgcc actcaataga aagctagtta tattccttga
     5161 ttgtactttt tcctcatatt cttctattgg caccatattg tttaagtagt gaccgttgca
     5221 atgggcacat ttcaaactac attgattgct tgtagtacta agagctagtg tctggttagg
     5281 ataagcaaac tctattttat ttccaaaatt ctttaattta atatcaaatg ctatttccat
     5341 tttttcagat agattcttat tcatagttta ttccttctta agtacatttt actcttctag
     5401 taatttcttt gctgttggga actgctctat attcgtatgt ggtaagaaac atgcagaaat
     5461 aaattcatcc atataacttg gatatacact aagttcaaca taagttattt gattacctat
     5521 ttcttcaagt ttattttttg catctttact tataagagct aagtaagaac caacaagtga
     5581 actattccct atataagtaa atttttctct atcaatatca ggaagtaacc ctataattat
     5641 tgagttttct atgtctaggt tgtttcctat accacctgct atataaaccc tatctaatat
     5701 actaaaatcc atacctaaac tatctacaag agtgtatgcg cctgaatata tagctccttt
     5761 agctctgatg aaattatcta tatcaacttc attgactact atatcatttt ctaaatcaaa
     5821 ttcttcttta aatgctaata catactctcc tatctcatgc tcattaaatc taactctctt
     5881 attatctaaa tccctgtata ttttacctct tctatctatt acccctttag taatcatttg
     5941 acagattaaa tcaattatac cagaaccaca tataccaact ggtgcacatt catcaataat
     6001 ctttaatgtt ggctctaaag tttctttatc aataattact ttctctattg ctcctgctga
     6061 tgctctcatt ccacagctta tccctccacc ttcaaaagca ggtcctgctg aacatgcaca
     6121 gctcatcata taatctttat ttccaaatac aatctctcca ttagttccta aatctataaa
     6181 taaaacattt tcttcacttg accatatccc tgctgataat acacctgctg ttatatctcc
     6241 tcctacataa cttgcaacag atggagctaa gtacacatat gcactgtcat ttacaaaaag
     6301 accaacattt tcacccataa gttttggaga ttttaaaaat ggtggtatgt aaggttcttg
     6361 tcttaagaag tctgtataca cacccaagaa taaacttgtc atagttgtat tcccagcagc
     6421 cactaatgta actacatctt ccttatctat tccatttctt tcatatattg tttttaatag
     6481 tggatttata gtttcctcaa ctatagcttt atgaagtgtc tctaatccat ttttcttagt
     6541 tgaatatata attctattta taacatctgc tccatatttt atctgtgcat ttccagaaga
     6601 tgctttatct actacctctt ttgagtatag gtctactaaa caaacaacta ctgaagttgt
     6661 accaatatct attgcaactc cgtataagga attttcttta tttccttgtt ctatatttat
     6721 gattgtaagt ttcttttgtt tttgaacata tgttattgta accttaaaat cactctttct
     6781 aaatacagtt ggcatttttc taagtatatc caatctaaaa tctatttcat tatatcctag
     6841 gttatttcta acatatcttt ccaatctatc tacatcactt atattatcat ctaaatttgg
     6901 ttcttccatt tctatgtatt tcttttttat atttgtttta aattgaaggt tatgttcttc
     6961 aattattttt ttagctcttt caaaaatttc cctatcctct tttttattac taccttctat
     7021 cttcatacca tgcatagaag atgatacttt agaaggaact tcaatttcta tatctgatat
     7081 tacctttgta caacaagcta gtatataacc ttgctcccac tcatcatctg ttatatgtct
     7141 tgttttttct gtatcaactt ttccattcaa taattttact ttacacttcc cacaagacac
     7201 gtttccatta caaggagcat ctatgaatat atctgcattt cttgcaacct ctagtaatat
     7261 atccccctca ttacaataaa cctctttatt gtttggtgta aaacttactt ttatcatgtt
     7321 atccctccca atttgttcta tatatgataa aaggaaagta tagctaaatt tgcgtgcact
     7381 caaattttac tatactttcc ccaattataa taatatctta atctttaatt attctaatcc
     7441 agcctcgtat tctttatcac ttaataacgt atcaagttca gccacatcag ctagttttac
     7501 ttttaatatc cagttttcat atgggtcttc atttatacat tctggctcat cttctaattt
     7561 ttcattaact tccaatacct caccattaac aggagatatt aaatcagaag ctactttaga
     7621 agactcaact actccaaaat ctactccctt agttacagta tcttctactt ctggtgtttc
     7681 aacaaacaat atttctccta attgatcttg agcatagtct gttataccta tatatacaac
     7741 atctccatca attactttta cccactcatg atctttagag tattttaatt ctggtaataa
     7801 tttcatttcg atagaccccc aatattatat tattgattaa attacttaaa tcatattaat
     7861 attatatcat attattttaa attttccatc aattacatta cactttccat agctaatgct
     7921 ggatgacctt tttcttctaa gaaagctaat actccttcag aatcgtcagc tatagtttca
     7981 tcacaaacca tatcacagaa gttttctatt ccagtcattt ctttaactgt agcatttaat
     8041 ttatcagcta cataatcttt taattctttt ggcatccaaa ctattctttc tggtccacct
     8101 tctgcataag cgaatttttt agaagatatg aaatgtcttc cgtgtcccat aaatcctgga
     8161 gtttgaactc ctccacctgt catagaagca agttctccga aagtcatacc aactggagta
     8221 acacttgcaa attctctgtt aactacaaca aatccatttg cttctggcat tataccacag
     8281 atacattcga agcatccaca agaagtcatt gggtcttcta gtatagagta taatgtaaca
     8341 gattcaactg caccttgaga tatttggttt acagtttcat ttactgaatt ccatacacct
     8401 gttctatcat ctaagcactc gcctttttct attggttgac aaggacctgt tgggtctaat
     8461 tctttagtag ccttagcatc taaccagcta actgctccac aaagaccaag tctttcagga
     8521 gtaacaacac aaacgtgagc tggtgcaaat gattgacata agttacaaga atagaaagta
     8581 tcaacacttt catcaactaa tgaagctaat ctttcatctc tagcattgta cttagctatt
     8641 gcttctcctt ttaattcaga aactttttca gcatctgtaa ttatagttat ttcacactta
     8701 tcaactactg attcaaattc atctaacatc ttagcatata aaacttctcc tatatgctca
     8761 agtctaaatc ctttatcaaa tgcatcttta gagattctta cccaagccat atctctttgt
     8821 ccaacgtgca ttacaccttc tatatagttt aagaagtagt ggaaacgtct ttcaagaact
     8881 ggctcgaaat cttcttgcat atttttacca gctattttaa ctattacagc aagtggtaat
     8941 cttaatacac catctgcatc aacttcatct atatttggtc ctattatttc tatcttatgg
     9001 tcttcaactt ctgttgcttc tttcttaaca acaagctccc aagcttcagt tctgtttcca
     9061 ccaaactcag caaacatatc gctctttcta attctctcac cttcaaatgc tgctgcaaat
     9121 gaaactggga taggtatttc agtaactttt atttttattc ctctagcttc taatgaagta
     9181 gctactatct tatcgtaatc tttttgagtt aataggttca ttggaacttc aagaactgtt
     9241 tggtcagtta taactgggaa tcctaaagct attgctccag cacctgcaga tacaactaat
     9301 tcacttaatg gtccaaatgc attaacaaat gcaggaactc tattagctgt gtactctaat
     9361 aatccgttta aatcaccagg agttaatcca ccgaatatta atgctgctct tactgcaaca
     9421 gatacaacgt gtataacaga agttacatca tatcctagag gtataactct tagctcaaga
     9481 cccattttta ctccagcttc tatagcttgg tctataactt taccaactaa gaaagttagt
     9541 aaacctttag attggtaatc ttttataact tttgctgctg attctgcatc tggacactca
     9601 ccaagaacaa ctgcaacacc aggtatatct cctgtaacta atggtacacc aagtgatctg
     9661 attatagggt cagttatatg acctgcacat ggctcactat atggagcatc catagttgaa
     9721 tattttaatg cttcaattac ttctgctgct aaagctgtag ctaaaccagc attaaaagca
     9781 tgttctagta aatgagttct atttattaaa gattttacta cttctaaagc accttctaag
     9841 tcccctaaag tgttcatctt ttgtcctgtt gcagcatata tacaaggtaa tgaatatgct
     9901 gtatcaggga aagcaacttt atgctctttt ccattctttt ctatagcttc agctaacata
     9961 gcttgagccg cacctaaagc ttgttctgac cctgtaaaga ttatattata tagattcatt
    10021 ctatttcccc ctttaatatt tacacctatt ttttattaac tataccctaa aaaccctaga
    10081 aagcaaatcc accttcattt aactctaaga aagagtctgc atataaagat gccccgttga
    10141 ttatgtcaca agattttaca gttttcatta attctgggtc acatggattc attatagctg
    10201 aactaaatcc attagccatt gccattgcta agaatgcact atctaaaaca ggtctaacat
    10261 gcttaggaca tccattagat acgttagata atcctcctgt agttaaaagt cccatctcag
    10321 tcatcatttt tatagcttct aaaacttcta cttgtttttc ttgcatacct tttataacta
    10381 agcataatgg gtcaaatagt atatcagttg gctccattcc taacattaag cctttttcta
    10441 atatttcttg gcagtaagcc atacgctcat cattatctct tgggatacct tcttttgcac
    10501 ataaacctat aaccattgct tcgtattcag ctgctatatc tattaaatca aatcttgaac
    10561 cagcatcagc agagtttatt attggttttg cattagttct atcataaact ttaagtccag
    10621 cttcgatagc ttttttatta gctgtatcta atgctatagg aacattgttg aactcagatt
    10681 gaagtaattt gattccccat gtcattattt cttcgccatc tctttcggca ggtcctatat
    10741 taaaatctat atagtgtgct cctgcttcta attgctcttt tgctcttttt aagattggag
    10801 ctgggtctct ttctgctaat gcttttctta ttgaaggtga tatacaatgt attctctcac
    10861 ctataatcat aaatttttcc atttataatg tcccccttta aatttaatat taatagttag
    10921 ttaatatttt attagtttgt ttggcatgct ttttcttggt atccttttaa gaacttaggt
    10981 aattccatag attcctctgg tcctataact acattccatc ctggtaagtt atcttctata
    11041 tcacctttaa gtactgcaac ttttcctgga atgataaggt ctttacaatt agttacttct
    11101 tctaccttac tttctttaat gaaagcagag attgagtttc caccaaattt accagcagcc
    11161 caagatgtaa gaactgaata tccacctgca tctggtatta ctaaccatac tggaactttt
    11221 gatctttcta tatcaccagc aactatgaag taagttaatg caaagtcaac agtaactaat
    11281 actggagaat tttcatctgg gttgttgatt ggataaatct ttggttcaac tctcattggt
    11341 ctttgtgggt cagtgtatat gttttgtctt agtgcaaata atggtaatgc cttagcataa
    11401 ctaatatcat ctattactat tatagaaccg tattttatag tgaatattga tgataaagca
    11461 acttccatca tagggtttga attagataat ctatttgcaa atactattga aggatatcca
    11521 aatgttctat cttgctcttt taaagctgtt cttctaactt gtatagcatt agtgtaagta
    11581 tcttttatgt tttctcctgt tacatctaat actaattctt tatatccagc agcttgaatt
    11641 aattcaactg tttcatataa ttcttctaag ctaccagctt ttacacctaa tggtaaactt
    11701 gctcctttaa ctacttctat catatctttg tagttctctt tagttgctcc atataccatt
    11761 ggtctagcat cttttaatac ttcaacagct tctttaacta cttgtgcatc atcacaagct
    11821 aaaatataag ctacttctaa tccactgcct tttattttat ctattaattt taaatacgca
    11881 tctttatctc cacaatactc taaaacagcc atttcaactt tcatttgttc accaattctt
    11941 acatagtcaa cttttttcat gttagctatt ttagcatcta ctgcttcatc actcatacaa
    12001 gtacagaatg aaactgcata tctatttcta cttactaatg ttttttcatg tctaaataat
    12061 actgtttctc cacctagttc atactcagat gcaccagcac caacttttaa agctttcatt
    12121 aaaggtgcag tagcctctga taattttgct atagcgtcat cagacatatg tgggcattta
    12181 cctacttcta cagctcctga agcaaccttc atagagaaag ccatacaagt tgggaatcca
    12241 caatccttac agtttttctt tggtgttaat ttaaatatat ctaaagcttt taatgccatt
    12301 attttttccc cctaacctta acaatttaat taagctaatg cgttaaccaa ttcttttata
    12361 gtttctattg attttggatg acgaagtata actgcgtttg aaccacctac taaaacactt
    12421 gctgcagttg aaacttccat tgctatagtt ctttcttctg gacaacccca atctggctca
    12481 tcttctattg gagctatagc ttctttaaca tgacctactt caaaagctac tggtgttatg
    12541 ataggcattt gtaatgtctt atcattttga ccaaatgctg caagtctaat tctatccata
    12601 gtagatgcaa cgtactcata tccataacca actgctgaac atccaacatt cataactata
    12661 ttttcacctt taacgcctag ttgagttaac aatacgttta attgtttagc taggtttata
    12721 tcaacagaag attcagctcc tactttatgt gcataagcca taccagctga tgctcctact
    12781 cctttgtagt tatcttctac tgctgacata aataagcagt tatgtccatc caatgtttga
    12841 gctaattttt caaataattt accatctttt tcatgatttc ctgaaccagc tacaactaaa
    12901 ggtaatttta ttgcttcaat tactgcctta gctacatcag cacattcatc aactgactta
    12961 tctaatccat ttgggtcaga accatcaaat tttaaacaaa taaaatctgc ttgtgtatta
    13021 gcttcaacat atttagccca ctctactgga caattagcta catctttgta taattcctta
    13081 tatgaatcag tccagctttc aggataaaca tctgatattt gtatccctat ttttggagaa
    13141 ttacctactt ctccatcaaa actataaaaa ggtaatacat tttccccacc taatttaatt
    13201 gctttttccc ctattcctac ttcaacttct gatatttttc cagaatattt ttgagtagac
    13261 attttaaatg ccatattttt ttccccctta agcctatttt aattctaatt tagcaaaaat
    13321 ttctttcata gccactttag attttgaatc ttcaggtaaa ttaactaaag gaatacctgt
    13381 agaatcatat tcatatatca attcatccat aggtacaaca ccgattagct ctaggttatg
    13441 cttttcaatt tcttctttaa caccatcatt cataacccca tttggaactt tatttacaat
    13501 caaaagtatt cttccaacac ttaactttag ttcctcagct aaatctctta ttctagcaac
    13561 agcttgtatg ctacgtcttg agcaatcact aaccaataaa agtgtatcaa catgtctagt
    13621 gacttttcta cttagatgtt ccataccagc ttcgttgtcc atgactaagt atttgtaatg
    13681 accagatatt ttgtttactt gttctctaag tatcccattt acaaaacagt aacatccttc
    13741 accttctgac ctacccatta ctaataagtc atatccttcg ccttcttcga ttatagaatt
    13801 taatctaaat tgaagatatt gtgcctttgt cataccacca gggaatgcat tgcctaactt
    13861 ttctctttga tttacttctt ctcttatttc tcctattgtt gcttcaactt cgatacctaa
    13921 tacttcattt atgttagcat tggcatcagc atctacaact aatactggtc cctttttgtc
    13981 cttaaccaaa taatcaatca aaagccctgt gagacttgtt ttaccagttc caccttttcc
    14041 tgcaacagct atattgtatc ccattttaag acttccccct taaatctagc cacagagcct
    14101 tatgtactct gtctgtctat tataatttag gtacagaatg aacacattct ccatgtacat
    14161 catgaagtgc ttccattagc ccttcagata aacttggatg tggatgtatt gcatccccta
    14221 cttgttctac tgttaatcct agatgaacag caagtgacaa ctctgtcaat aaatctgtag
    14281 catgaggtcc tactactgct gcacctatta tcttatcagt ttctttgtca gctataactt
    14341 ttacaaatcc ttggaaatgt cctatagctt gtgcttttcc aagacctcta aaatcaaatt
    14401 gacctacatt gtattctaca ccttcagctt caagttgctt ttcagtctta ccaacaccag
    14461 caacttcagg ttcagtataa acacatcttg gaattgctct ataatctact accttagtct
    14521 tacctaaagc attttcaact gctacgatac cttctttaga agcaacatgt gctaggaaag
    14581 gagtatctat tatatcgcct attgcgtata taccttctac attagtttca agatgttcat
    14641 taactactac ttttcctctt tccatttcaa ttcctatatc ttctactcca gaattgtcaa
    14701 ggttaggtct tctaccaaca catactaatg catattgtgc ttctataacc tttccattag
    14761 aaagagttgc aacagcctta ccatcaacta cttcacaagt ttgtactcca atacctgtaa
    14821 taaccttaat tttatctttc ttaaattgtc taagtagttg tttagctaca tctttgtctt
    14881 catttaatag tatttggtca accatctcaa ctatagttac ttctgttccc aaggctctaa
    14941 agaactgccc tatctcacat cctataactc caccacctac tattaacata gactcaggta
    15001 tctcttcaag tccaagaact tcatcactag ttattacaac ttttccatca tatgggaaca
    15061 ttcttggtac aactggaaca gaaccatttg ctagtattat cttatctgct tttatagttt
    15121 ctactgttcc atcatcttta gttacttcta tagtgttttt atcaattaat tttccaaaac
    15181 cattaactaa gtttactcct cttttttcaa ataagaactc tataccactt attaattggt
    15241 ttacaacttt attttttctt tccattatgg cagtgaagtt tggcttaact gtaccatcta
    15301 tttctatacc aaaatccttt gcttctttta cagtattcaa tactcctgaa gaagcaagaa
    15361 gtgctttagt tggtatacat cctgcattta agcaagttcc tccaactctt cttttttcaa
    15421 caactgttac atctgcacca agcatagaag cttttatagc tgctacatat cctcctggtc
    15481 caccacctac tactactatc ttcatcttta attcccccaa acctaatata atctacaatc
    15541 ttgcaatact ataaacctgc ggcatccagt atagcaggta tattctcttc tgccccaatt
    15601 gccattatat gaacaccatc acataaatct tcttctttta attgtttgat aagttcccct
    15661 gccattttta ttccttcact tacccagttt tcttttccag ctgctcttaa tctttcgatt
    15721 tgttcatctg gaacaaatat tcctggtaca tttgcagtca taaatttagc cattcctggt
    15781 gattttaaag gtactattcc tgctaatacc ttgcaatcca tatcccttgt taattctcta
    15841 aactttctca ttgtatttat gtcataaagt gcttgtgttt ggaaaaattt agcccctgct
    15901 tttattttct ttttcatttt taataattga acttctattg gagaatattc tggagttact
    15961 gatgctccca gataaaaatc tggagaacct tttaacttat taccagccat gtccgtacct
    16021 gccattaaag tctcagcagt ttgaagtatc cccacagaat ctaaatcaaa cactccctta
    16081 gcttgtggat ggtctcctac gctagtatgg tctcctgtta gtgccaacag attgtttatc
    16141 ccaaatacac ctgctgacaa catttctcct tgtatagcta ttctatttct atctctccct
    16201 gttatttgaa taactggctc taatccagca tcttttaata atttacatgt agcaagagaa
    16261 gttgctctca taactgccga ttgaaagtct gttacatttg ctgcatgaac tcttccaact
    16321 aacggctttg cacattctat taaatgtgaa aggtcagtcc cttttggagg agccatttca
    16381 gtagtaactg caaattttcc actttctaat gtttctctta ataagctcat ctctaatccc
    16441 ccttttaacc ttctaaaaaa aacttttatg aatttgctgc tgcttctttc tttttctttg
    16501 cacttaaact tcttggatta ttttgcttag aatagtcttt tggtggtctt atttctgcta
    16561 agttatctaa ttgtcctata gcttctagtc tttcatatat ctttatccaa gcacaatcat
    16621 tctctgagtt tacttcacac ttaccatttt tagctccacc acaagcccca ttcataagac
    16681 ctttagcaca catagttact ggacatatcc cacctgtcca accaagttca cagtctccac
    16741 aagccttaca ggcttcttca tattctccaa ctctttgtac ttcccctata aacaaagtat
    16801 tgttggctgg ataaactggc ttatctttta aattcttaac tattgtctgt gtaccatctc
    16861 cacatgctaa agataaaact gcatcagctt ccttagtctc ttcctttaga gcctttaaat
    16921 cctttttaga ctttaaaagg ttacaagcag ggtcaagcat tacatatcct aagatttttt
    16981 tgccttcacc ttcaagagtt tccttcattt ttaaaacttc ttcttcccca ccacttttac
    17041 aagttgctgc acattgatta caaccaacta aaaccagctt gtcaaaatct tttaagtacc
    17101 ctagcacttc ttctaatggt ttattttcag aaattatcat atctacttcc cccctaaacc
    17161 ttccacatat atatatctat ttattgtttt gtaatttaca agccttaact acatgttttg
    17221 caagtataga tgttgtaact gaaccaaccc ccgctggaac tggagttatc attgatactt
    17281 tgtctaaaac tgcatttgta tcaacatctc cacataaatt tccttcttca tcaacattta
    17341 taccaacgtc tataactacc gctccatctt taacaaaact ttcatcaacc atcttagctc
    17401 taccaatagc tgctattagc acgtctgcct ctgctgctac acctgataga tttttagttt
    17461 ttgaatgaca tattgtaaca gttgcatgtt cacttaataa aagcattgat actggctttc
    17521 caactaccat tgatcttcca agaactgtta ccttacttcc ttttaaatca acattatagt
    17581 gttttaatat ctcaactact gctgtaggtg tacatggtgg aaatccactt ttatcccctt
    17641 ccattacctt agcagagttt attggactaa aacaatctac gtctttttcc ggtgcaataa
    17701 catattttat aacttcttca tttaagtgtt ttggaagagg tctaaaacat aatataccat
    17761 taacattttt gtcatcatta actctcttta atacatcaat atattcctct tgtgttatat
    17821 cctcagctaa ctctaacact tctgttgtta tacctatgtt ttgacatctc tttagtgctc
    17881 ctctttcata agataaatca tcacttcttg ccccaactct aactattgtt agtttaggat
    17941 ttataccttc tttaattagt aaatcgactt cttttattaa ttcttcacta atcttatcag
    18001 ctacaggttt tccttttata atttgtcctt tagttgacat tccttccata cattcacaac
    18061 tcccctacaa gcaaacttaa attaatttat tctatatacc acatattaat aacatagtat
    18121 ctactttcgt catttaaaaa ttattttcca agagcttttt caactttggc ataaacttca
    18181 tcacaaattt ttacaccatc ttcaactaac ttatctactt ctgtttttac cttattaaca
    18241 tattctttat cttgtataga atttatattt ataactacat ttaattgtcc accaagtata
    18301 gcagctctta aacactgaac tcctacacca acatcactta tagcaagtct tgaaccttta
    18361 tcaactaaat cttcatgtaa cttaatagct tcatagcaaa ctcttactat gtttaatggt
    18421 acttcacaag caacttttaa tgccttttcc attgtttctg cttttattct tttttcttct
    18481 tctgtttctt taggaagccc gtaagctttt gataatggta agaaacattc tgcatcatca
    18541 tctatcattt ttaataaatc tttttctaac tttcctgcct tacttaatat ctcttttaca
    18601 ctttcttcat actcagcata cttcttcttc cctattgtaa ggttacatac catactacct
    18661 aaagccatac ctattgcacc tactagtgca gcagctcccc caccacctgg tactgcttct
    18721 ttggaagata gtacttctac aaaatcaaca caagttttat ctgctatttt catgataatc
    18781 ctccttgaaa ggatgtagga aaatatctca aattgttcga gatattttac gaactatttt
    18841 aagatatctc ctactcttta tttataaatt aatttcctta gaataatcca gctattactc
    18901 cattttcatc aacatctatt ttttctgctg ctggaacttt tggtaatcct ggcatcttca
    18961 ttatttctcc agtaagtgca actataaatc cagctcctgc tgaaattgtt gcttgtctta
    19021 cagttattct aaatcctgtt ggtctaccta atttagtttg gtcatcagtt aaagaatatt
    19081 gagttttagc catacatact ggaactttag taaatccaag tttttctaag ttagctattt
    19141 ccttatcagc ttcaggagta taatctacac catctgctcc ataaatctta gtagctatag
    19201 catttatctt gtcttttata gataaatcat cttcatagca gaatctaaag ttattttctt
    19261 cttcatccaa taatcttaaa acttctttag ctactgcaat tccaccttct ccaccttttg
    19321 cccatacttc agaaagagca acattaacac ctaattcctt acacttgtct tcaacaagtt
    19381 taagttcagc ttcagtatca agtgggaatc tattgattgc aacaactgct ggtaatccat
    19441 atacttgagt tatgttttca acatgtttta ataagtttgg aagtcctttt tcaagagctt
    19501 ctaggttttc gttatttaag tctgctttag caactccacc attgtatttt aatgctctta
    19561 cagtagcaac tattataaca gcatctggtt ttaagtttgc cattctacat tttatatcta
    19621 agaatttttc tgctccaagg tcagcaccga aaccagcctc agttattaca taatctgcaa
    19681 agtgcatagc cattcttgta gctataacag agttacatcc atgagctata tttgcgaatg
    19741 gtccaccgtg tacaaatgat ggtgttcctt caagagtttg aactaggttt ggttttaaag
    19801 catcttttaa taatgcagcc atagcaccat tagcttttaa ttgtctagct gttacaggtt
    19861 caccttcgta gctgtatcca actattatgt ttcctaatct ttcttttaaa tcagatatat
    19921 cacttgccaa acagaacgct gccattattt cagaagcaac agttatatcg aatccatctt
    19981 gtctaactgc tccatcacct tttttaccca taccatcaac gatatttcta agttgtctat
    20041 cgttcatgtc tacacatctt ctccaagtta ttttcttagg gtctattcta agtgcatttc
    20101 cttggtgaat atggttatca agcatagcag ctaaaagatt gttagcagct cctatagcgt
    20161 gaaaatctcc tgtgaagtgt aagtttatat cttccatagg aacaacttgt gcatatcctc
    20221 caccagctgc tccaccttta acaccaaata ctggtcctag agatggttct cttaaagcaa
    20281 ctaatacatt tttgtctagt ttagcaaatg catcagcaac acctatagta gtagtagttt
    20341 ttccttcacc tgctggagtt gggtttatag ctgtagttag tattaactta gcttttttac
    20401 cagtctctct ttttagtaga ttgtagtcaa cttttgcttt gtatttacca taaagttcta
    20461 catcatcttc acctagtcct aatttttttg caacctctct aatatcttga ggcttagctt
    20521 cttgagctat ttcaatgtca gatttaaatc ccataactga atctcctccc acaacctaaa
    20581 tagatttaaa atataatttt tgtaaaaatt gtattaatat gtaaaaatta tattttataa
    20641 gataattata catgtaaaat tacttaaaaa ctagctacta agctattaaa atattcaaac
    20701 ttaatagcta gttatttatc taattactta cttattacct atctattgcc tatgtctaac
    20761 ctttaaccaa cttaaaatcc caaatcaaaa tttctatcta ttttacttat ccttaagtga
    20821 cattaattta tcttttattg ctttaatctg ttctttcgtt gtttcactag aatcatatgg
    20881 agattgatta gctctatcag aatcaataac atcttggtta taataaataa atcctaaaga
    20941 ttctccatct tctaagtttt ggattataaa ttcttcatct tccttatttc taattttatt
    21001 tcctactaca aatactttat ttacacctat atcatgtcca agtttcttaa cttttctata
    21061 agtctgtaga cttctctctc ctggttctac aactactata aaagcatcta caccttgggc
    21121 tgttcctctt cctaagtgtt caattcccgc ttccatatcc ataaccacta catctttatt
    21181 ttgtagtatt aaatgtgagc agagtctctt caaaagaaca tgctctggac atacacatcc
    21241 agtaccacca gaatctacgg ttcccaatgt aagtaatctt actccattgt attctttaca
    21301 gaaattttca gggatatcgt ctactttagg attcatctta aacatttttc caaatgaacc
    21361 tacagaagca gctgttctat ctgaaactaa ttttttcatt tctgatatag gtactattga
    21421 ttcatatact tcttttggaa aacctagtgc taaagctaaa ttagcatcag ggtcggcatc
    21481 aacagcaaca actctgtatc cgtcttcagc aaacatccta gagagcattg aagaaaaagt
    21541 cgttttacct actccacctt tacctgttat tgctatcttc ataatcccta ccacctttta
    21601 ttactatttg ctttttagta gttaatttta cagttaaata ttttttatac tctatatttt
    21661 tatacactaa aaataaacta cagctagtcc ttacaggaac caattaaatt taccctacta
    21721 gcttttcaaa gcagcatgta tagttttatt attctttaat attgtttata ttttatttat
    21781 tcagtatatt cattatattt tgatgttctt tactttaata tttgacttta ttcaacttta
    21841 ttcaaccaga aacttcttat aaaaacgttt attctataac ttcactattt tttaagcttc
    21901 agccttttct gccatttttt cttcaactaa tgcttcaaag tgaacacgtt ttttctctat
    21961 atcttctatt attgtagctg ctaactcttt tggatttgta tttacagtaa atgtagctcc
    22021 aactttatct tttaattcac tagttaatat atccactgct cttgaagaac cagctattgg
    22081 aggcattatt cctaaataag tgtctatacc agaagctaca acatagcatc ctatagcaac
    22141 tgctttttct gacatccact ctggagctat acctacaact ggaaggtcac acatatccat
    22201 atctaagtaa ttagctgctg ctccaactaa atctagtata cggcttatat caacacaaga
    22261 acccatatgt agtactggtg ggattcctac taatttacag acagttgcaa gacctttacc
    22321 tgcatatttt tcagccgcat cagattccat taaaccaaac ttagctgctg ctgaagctcc
    22381 acaacctgta gtaactacta ttatgtcgtt tttgattaat tctttcatta ttgttacgtg
    22441 agcctcattt gaaactcctt tagcattgtt acatccaact acaccagctg ctcctcttaa
    22501 tactccagct tttaaacagt ctgttaatgg cttaactgtt cctgctgggt ctatatgaga
    22561 gtttacaact ctgtctaatt gatttactac tgcttctaca ctatacccaa ctgttgcgcc
    22621 tgattttaat tcaggtataa gtacttttga tttatctcta tttttaaagt ttaaaatagc
    22681 ttctttaact attgctttag catcatcaag tgcttgttct tctctaaatt caatataagt
    22741 tgaacctgtt atcttagctt ttggagaagt tgttacaaat ttagtatggt aacaatctgc
    22801 aactctagct aatgcaggga atatacattg aacgtcaact attacagctt caactgctcc
    22861 agttattatt gcaagttctt gttgatggaa gtctcctgct atttttacac catgtctcat
    22921 tgtaatctca ttacctgtac aacacatacc tgctaagttt attccatcag caccaacttc
    22981 tttagctaaa gctactaagt ctggctcttc tgatgctaaa actatcattt ctgataatgc
    23041 tggctcatgt ccatgtaata ttatgttaac tttattttct tctaatactg ctaaattcgc
    23101 ctctgttctt cttggcactg gagttccaaa taagatatcg cttagacgag tacctatcat
    23161 agaaccaccc caaccatcag ccatagaagt tctaagactc atgtgtatta aactatctat
    23221 atcaccagta catcctatat gagtagagtg cattattgtt gcaatttctc tatctatagc
    23281 tcttggctct atagcgtatt ctttccatat tttttgtctt ggttctggtg cattttttat
    23341 aaatcttaat gtcccaaaag gttttccaaa ttccattaat gcaatttctg ccacttcatg
    23401 tgctacatca tagatatctc taccttctgt ctctacatcc cattctttag ctaaagtaat
    23461 taacttagct tcgtctctta ttttatagtt tccatttgga tcagctaatg ctaatgtatg
    23521 tgctatatct ctagcatggt ctgaatgggc agctgtccca ccagcaacca ttcttgcaaa
    23581 gtttctacct actatagtat gctcatctgc tccacataac ccccttggag tcttagggct
    23641 tattctacat ggtcccattc cacatattct acagcaaaca ccttgaagac caaaaccaca
    23701 ttgtgcctta aagccttctt ttctatcata cattgtttct acgccttctt ctctagcttt
    23761 agctatcatc ttctggctgt ttaaatcaat tgttaacatt ttttcatcca tattcctatc
    23821 cccttttttt gatttttaaa atcccccagg tgactgactt catgtcataa aataataaaa
    23881 taaatgcttt ctaagttgat tatatactaa ttttcagtgc tttgctttag atttaaatat
    23941 ttttttaaca aaattagata atacataaaa attttatttt ctacacttaa tttaagttga
    24001 ttttatcgct tgtttttggc tatttgttaa tatctaaacc tacttttttg atacttcagt
    24061 ttaattaaat tatgttttga tttttttaaa tcaaatttga aatttttcta gtcattttta
    24121 tattattgca gattgtttgt gtatttatta tctttcacta ctgtttggac ttattacaga
    24181 tttttactcc ctaaacttaa ttagtaaaaa tttcttagaa tagcattttt tggactaatt
    24241 tttttgaatt tttttatttt tcctacttta atattaatta tagcttcaaa ttgaacattt
    24301 gtatatattc atttgaagct ataattattt tttatataaa acttttttaa tacagcatta
    24361 atttagacat tttgactatt ttttctactt taattcattt atttcaagcg atttatccta
    24421 tttcatattt ttagctacaa tagccccata tgcaatagaa tataatttag attcatgtga
    24481 atcagctctt gacactaaaa ctataggagc ttttgcaccc ataattatac ctgctgattt
    24541 agagtctgcc atatatgtaa aggctttccc tattccatta ccaacctcta ttgttggaac
    24601 caatatgata tctacatctc cagatacttc acttttaaac ccttttatct cactagcttc
    24661 ttttgaaaca gctaaatcaa aagccagagg accttctaca attacatttt caccaaattc
    24721 tccttcttta caagcttttg ctaacatatc tgcatctaca gttgcttgca tcttaggatt
    24781 tactttctct ttagctgcca aacatgcaac ttttacagtt tccattccta aagcttttgc
    24841 agcttttata gaatttttta atatctttgc cttttgttca taatcaggag ctatattcat
    24901 acctccatct gtagttataa gtaatttatg gtatttttct aactcatata ccataacatg
    24961 acttaataaa ctatcagttc taagaccata gtctttattt aacacttctt ttaatatgac
    25021 agatgtatct aataatcctt tcattacaaa gtcagcttct ttacttgata ctaatttaac
    25081 tgctatttct gcacactctt tgatatcatc cttatccact attttgatac cacttaaatc
    25141 aaatcctatt tcttttgata tttctcttat tttgccttca tttccaacca atataggagt
    25201 aattatattt ctttctacag catcctttat tgccattaaa acttctttat catgagctgc
    25261 tgctacagag agtacaattt tctctcctcc cttaagttgt tctaaaatat catctaattt
    25321 ttttatcaaa agtaacccct cccttatata tatttaacta aataatacca tatttgctat
    25381 accttttctc aatttatttt ttatgttata aagaatttat ataactaaaa gacagtttaa
    25441 tattttattc tgggatttca tcaatagctc ttaaaagctt tgcaggaaca ccacctacaa
    25501 tagtattttc tgcaacattt tttgttacaa ctgctccagc agcgataatt gcattatctc
    25561 caatagtaac tcctggaact acagttacat tagcaccaat ccatacattt tttcctattg
    25621 tgattggaga aggataagtt gtaccacgtt tctctggtgc aataccatgg ttaagtgtag
    25681 ccaataccac attatgccca atttgcgtat tatctccaat ctcaatcccc ccctggtctt
    25741 gaaaatggca actgctattt ataaaaacat tttttcctat tgtgatgttt tttccacaat
    25801 ctgtataaaa tggtggaaac ataaaaaatg attcatctac cttttttcca attaactcag
    25861 aaaataaact acggacttct tctggttcat gataagaaga atttaactta cttgtgattt
    25921 tcattgcctc attactcaac ttaaccataa attcatgtat ttctgattta ctagaaacag
    25981 gttttctttg attgatatgt gttaaaaatt cattgatatt cattttatac atcctttcaa
    26041 tatgtgcttt aatttatttg tacattctaa ataatatttt aataaaaatt aatttaattt
    26101 atttttatta aaatatcatt gcttctttta gtgtaatata caattatatc tatatcaaat
    26161 atctatatta aatacttaac tatacttaaa aagcatataa attgatataa ttatataata
    26221 aataagttaa tattgaggag ggataattat ggaaataaga gtattaagat attttttagc
    26281 aattgcaaga gaagaaagta tatctggtgc ggcaaccttt ttacatataa cacaaccaac
    26341 actttctaga caaattatgg aacttgaaga agagttggga gttaaattat tggttcgagg
    26401 aaatagaaaa atcaccttaa caaatgaagg taggctttta cataaaagag cagaggaaat
    26461 tatatcatta gttgataagg ttgaattaga atttgacaat cccaatgaga ttattagtgg
    26521 tgatatatat ataggaagtg gtgaaactga agctatgcga cttatagcta gaactgccca
    26581 tcattttcat caaaaatacc ccaatgtaag atatcacctt tttagtggga atgctgatga
    26641 tgtgactgaa aaattagaca agggactgct tgattttggt attttaattg aacctgcaaa
    26701 tatacagaag tatgattatg cacaaattcc tgctactgat gtatggggtt tactaatgcg
    26761 taaagatagt gcccttgcat cacaaagcac aataaatcca aaagatttat gcaatatacc
    26821 tcttattatt tcaagacaga cacttgttga taaagcaatt tctaaatggc ttcaaggcag
    26881 taatgaaaaa ctaaacatta ttgcaacata caatttagtt tataacgctt ctcttttggt
    26941 agaagaaggt ctgggctacg ctctgtgttt agatagactc gtcaatacta cagaaaacag
    27001 taatctttgt tttagacctc tatctcctcc tttagaagct aaattaaata ttatatggaa
    27061 aaaacatcaa atattttcaa aggcagcaga aaaattttta cttcaaatac aaaaagattt
    27121 ttcttctatc atgttggata catctaagtt ggatacacct aaagaaaaat aaatcataat
    27181 aaaaagtgag agtgtctcaa aatgaacttt gcactgtata aggcactctt ttttatacaa
    27241 agtaaaatat atgttcatca tttcagcact aaaaaagagg cttatctcta ttttgagata
    27301 gccccactta accaataaaa ttaaatacaa cttaacaata tccttactta tttattccaa
    27361 gttctcttag acaagcaatc gtaatttcat cttgtggatt taattcataa gctctttcta
    27421 tatagtaagt tgcatcatca atatcaccat tatttaaata agccattcct aaattgcaaa
    27481 gtatttctgg gtcttccttt atcttagccg cttgttcaaa atactctatt gctttatcta
    27541 aattattctt ataagcctca caaagcccca attccactat agtatccacc tgattaggct
    27601 ttattatcaa tatcttctca agatacatct tagcctgctc tatttcgccc atacccttat
    27661 atccaagtgc tatcataaac agcaagttcc accaatccat atgttcttct tctaatggaa
    27721 gtaacttctc taatccttcc ttaaacttac cttggaatac aagtgtatat ccttcttcat
    27781 attgaacttt aaagtccatc ttgcccagat tctcttgtac ctcagctact aaatcagcat
    27841 ctaaccctag ccttaacgct tcttcccaag tcagcttagt ctttaaatat tgaccttgat
    27901 tataataatg gtagcctaag tgataataac ctaatgcaaa attctcatca acatcaatta
    27961 ccttttcaag cttagccatg gcttcaagta agaatgcatt cattgcctta tcatccatat
    28021 ctttttgata ctgtttagct atctcttggc aaacaatcgc atagttatac attgcatcta
    28081 aatcatctgg atacattgtt ataaatgctt ttatatatat taaagaatct gtatgttcac
    28141 caatctcaaa gtattttctc gacatatatc ccatatacga tttcaaatcc aaatcaatat
    28201 cttttgataa agcatttaaa aacttctcat actctgcatt gtttttaaac ttactatcta
    28261 ttccaattat atatatcatt gcatctgcta tagacattga atttaactta tcttgagcag
    28321 tcttttcttt tatccctttt actaatacct cattttttat aggtacatct aatccacctt
    28381 ttggcacttc atagcttttt aatttaaact ctccaccatg ttttatagtt ataaaagcta
    28441 attcttctgc tttttttagc aaatattttt ctatcttaaa atccatttta tcctcctaaa
    28501 tacttaaatc tctactttat gagttttaga tttctcaaat atttttttat catcatactc
    28561 aaataagact acattttcta cttctcttat ttctgtattt atatcatttt ttaaatccac
    28621 aacattaaat ctaaatggct caaaatgaac atattttatt atatgtttag ctggtttatc
    28681 aacaaaacta ggtaaatatg ttgataagtt atcattatct tgtaaaaata catggcatct
    28741 attcttaaaa tcatccacat ctagcttatt aaaaaactgt ggtatatttg atgttttacc
    28801 cagtgatata tagtcatatt ttaatatatc gttgaataca tcattatcta tgttgatttt
    28861 ttcattatag aaatccatca atattttata taactgattc tttccttgtg ccaaatcaaa
    28921 gtagccattt tcatcaaaat acttggcaaa aacctcaaag aatttaaaag ggcttttttt
    28981 ataaaatctg cctataatat atctcataga taacacaaag tttttagaat tgtagtatct
    29041 ctccagtata tcttctatat cctttagctt caaagtttct tcatagctta tataattatt
    29101 gtataaaaac tcatatggag cataatcctt atatttaaaa ccatgctcat ctgcaacttt
    29161 tctcatacct gtccctttaa tcatctttaa aaagcctaat tgcagatgct ctatacctag
    29221 attaaataca tcattaaaag aattttcaaa gctcttataa tcttcataag gcagtcctgc
    29281 aattaagtct aagtgttgat gtatatttct ataagatgct acagtttgta caacatgaga
    29341 caacttttta aaatcatccc ttctacctac tgcatcaagt gttttttcat tcgttgactg
    29401 aacccctatt tcaaattgaa aaagaccttc tttacaatca gccaaaaact caagcatctt
    29461 atcatctatc aaatacgctg taacttcaaa atgataagtt gtatatccat tatcattttt
    29521 cataagaaaa tccatgattt ccatagccac ttttctattt gcattgaatg tcctatctat
    29581 aaactttatt tgtgatactc ttgcatctat taaagctttt aaatcactct ttatcctgtc
    29641 taaactaaaa taccttagac ctggaatagt agatgataaa cagtattggc agttaaaagg
    29701 gcaacctctt gatgattcat aatacactat tctattttca tattcttttg gattcaagtt
    29761 ttcataagga ctaggaatct catccaaatc ttgaagtaat tccataggct tattcactat
    29821 tatctcattg ccttgtctat atacaagtcc atttacatca tttatttcca tctttccttg
    29881 caagtgaagt actaaatctc taaaaattaa ttctccctct ccataaagta tataatccac
    29941 aaaatcatac tttttcattg cctcataact atcataactc acttccggtc cgcccaaagc
    30001 tattttagta ttaggtctaa ttttttttag attatcacaa agtttaacta tatctcccac
    30061 attccagata tatgttgaga ataatattat atcatattca tttttatata tatcctttaa
    30121 aatatagtcc aaatcgttgt ttatcgtgta ttctttcata ttaacttcta tcaaatctct
    30181 tacaaattct tttagatatc ttatagccaa atttgtatga ataaacttgg aatttaatgt
    30241 tgttaataaa attttcaaat atttctcacc tctttttaag attatactgt ctttttgtac
    30301 cccttatagg ttaaaatagt atatgtaaag tctaaataat atctatataa ttaatttatt
    30361 aatttcaagt aatgtgagga aaggaagttt tttatggaat taattgaaaa agtatctatc
    30421 aactctttat caaaaagaga tttactactt attataaagt ctcttgaata cactaatgaa
    30481 aatactcaaa ttagtgattt cattgacctt aggaataata tagtaaagga attatgtttt
    30541 ttaactgaca ctaaggaaaa agatttttta gattatctag aaaaaaatag cacactctaa
    30601 taacctaaac ctttttaaac tttaatatat aaatacctga tttagaattt atttttatgc
    30661 ttaatatatt atcattaaag gttttcgaca taactgttat ttgattaaat atagggtcta
    30721 ctatagtatt accttcttca agtcttataa gacttcttgg gtctatgctt ttattatatg
    30781 ccttaactcc aattttatat tttctcaatt taacaacata ttcggacata gaaaaaataa
    30841 aatctatatt atcttcattt aatttactat cttcacccaa aatggataat atagattttt
    30901 ttatgctatt aattccttca ttttctacag ttctatcttc catatataca tcatatagaa
    30961 catttaaaac atttaataaa atactttctt catcataatc ttcagatttc aaatcaatgt
    31021 acttatcaat taattgtatt gctttaatcc ttgccatctg aaactttaca acatctgatt
    31081 tgtctaattc tatagaaaat cctattatct tgtctttaat actctgtagt aattcgacat
    31141 actctatttt actattattt ataagattgt gcataatgga attatacacc acagaaccta
    31201 ataaataatc aaataaaagt tcttcttttt taaaataaat aacatacttt tttattagtt
    31261 taataacctc atcttcactt ttttgaatat tagtatttga taaaattagt ggtaataaaa
    31321 tatttaaaaa attgatattt cctatttttt ctccataaaa tctatagtaa ataagttcat
    31381 ctctcagtct attttcgtct atatacacac atctttgcgt tttatcatct attaaggatt
    31441 ttgtatttat tatagttaaa tctttaagag ccctacttgc actactaaaa gaagatgtgt
    31501 agactttttt tagtaattcg tcattcataa atatatcctt tctgaaaaat tgaatgtaaa
    31561 taaattgtta ttaaaaaaaa agagacagcc cgtttaagaa gctgtcatat atagggggat
    31621 agtagtgtac aaaaatcatt gtaatctact atctatatat tattatacga tactactttt
    31681 ttatatgtaa agcatcagtt ttcgacatcc ctatatttta cagttataca aattgcgtac
    31741 taagttcaat tttattcttt ttatttcaac attttatcat gctacattct ttcaggagcg
    31801 tgcattccca acaatttcag agaattttct aaagtttgtt ttgttgctaa aactaatgca
    31861 agtcttgctt ttcttatttc caaattttct actaaaattg gattgtcatg ataaaattta
    31921 ttaaacgctt gagctaaatc tagcataaat ctagtcacta tatgtggctc atttttcttt
    31981 agagcaagta aaatattctt attgaatgat tctataactc taagaacttc tgcactatct
    32041 tcatcagcta atagactata atctatatca gaagtcactt ctacttctgc ttttcttaaa
    32101 actgcacagc atctagcatg tgtatactga acatatggtc cagtctcacc atcaaaacta
    32161 agcgttcttt cccaagagaa ggtatagtct ttaattctac tattagataa ctcttggaat
    32221 actactgccc caacacctac ttgcttagat atttcatcca catttttagc atttgggttt
    32281 ttagctagta taatttcctt agttttatct atagcttgat ttaaagcatc ttctaaaaat
    32341 actactcttc cttttctagt cgacatagta ccttcttcaa gtgcaaccat accaaatcca
    32401 acatgaatta aatcttttgc ccaatcatat cccataagct caattacctt aaaccattgt
    32461 tggaaatgta agttttgttg agaaccaact acatatatac acttgtcaaa atcataagtt
    32521 tcttttctat atattgcagc agctaaatct cttgtcatat ataacgttga accatcattc
    32581 ttagtaataa gtgctggtgg cattttatac tcttctaaat caacaattct tgctccttta
    32641 gactctttta aaagattttt ctcttctaat agttcgataa ctctgttcat tttatcagaa
    32701 taaaaactct ctcctgctaa agaatcaaat tctatatcta aaagtttata aactctgtta
    32761 aactctttta aactctcatt tctaaaccat tgccataatt cttgtgcttc ttcgtcacca
    32821 ttttctaatt tattaaacca tgctcttgct tcatcttcca tttctgggtg ttgttcagct
    32881 tcatcatgga atcttacgta taattttagt aattctggaa ttggattaga ctctactact
    32941 tctttttctc cccacttttt aaaggccact attaacttac caaactgagt tccatagtca
    33001 cctaaatgat ttattcttat agtcttgtat ccttgcgaat catatatctt atataaagca
    33061 ttacctataa ctgtagttct tatgtgccct atatggaaag gttttgctat atttggagaa
    33121 gaatattcaa ctattacagt tttatctttt ccaaaatcac tatgtccgta attttctttt
    33181 tcatctaaaa cttttttaat tacagtttct gctaattgag atttatttac aaagaaattt
    33241 acatatccgc ctaattgtat tacttttgtt atctctccac ttggttctat actttccgcc
    33301 aattcacttg ctatcatgtt tggagctttt ctaaacactt tggctaattt aaagcatgga
    33361 aaagcatagt cccccatatc cttatttggt ggaacttcta taagtgcctc tatctcttct
    33421 ttcgataaat cttctatttt ttctttaagg caatttgata ttgccacctt aaaatcttgc
    33481 attctgttgc tcctctctac attgttcctg gtttatattg tgcttatctc tttatgttaa
    33541 atcaaatttc gtgttttttc aagtatttca ttacagtgta aaacacttta gtaattatgt
    33601 tttctgtata tattaaaaat actataattt cagtttttaa tcacactatt attgattttt
    33661 tataagactt ttttctattt tattgtttct tgtactatcc agttgacttt atatactttt
    33721 tttaatcttt tatctgtttt catacatcaa tgtcagcttt atatacattt ttttaatctt
    33781 ttatctattt tcatacatca atgtcagctt tatatacttt ttttactttt ttatctattt
    33841 ttatacatca atattagctt tatatacttt ttttactctt ttatctattt tctataaatc
    33901 tttttcactc ttaactttta ttccttcttc ttctaatagc cttgctgtta tacctttccc
    33961 tggtatcttt ttacctgaaa aactaccatc atatattaga ttacatccac aagaggggct
    34021 accctctttt agtattgctt cgctacatac atataaattt gctattttta gagtttcatt
    34081 agcccctctt acaaagtttt ctgttacatc cttattatct ttagtaatta cttttccatc
    34141 aactatctca gcaggtggtc ttggtgtaga tagaccacct aactgctctg ggcatacaag
    34201 tgtaaattct ttgcctttta aatattcctt gactttttca ttatcattat tatcgccact
    34261 atatttacaa tttattccta ttaaacatgc agataccaat atcataaaaa tgtgctccta
    34321 tacaaattat atatattctc atacaaatta ctaatgtatt cttatacaaa ttattttaat
    34381 ttaacaattg ttactcctgc tccaccttct ccatattgac cacctctttg tgattttaca
    34441 tgtctatttc ttcttaaaat gtcttgtagc cctgctttta atactcctgt acctattcca
    34501 tgtattactg ttacagactc taaacctgcc acatatgcat catcaagata cttttctact
    34561 tccattatag cttcttctag gtttaaccct ctaaggtcaa cttcattttt tacactacca
    34621 gattttgacc ttataatgtt tctcgttgat ttagttacat ttgttgaaac atctttgcgt
    34681 gttttttgta aagatttaaa aggtagagtc atcttcatta taccaatttg cactactgct
    34741 tcttttctct ttttatctac tgaaactaca ctaccatttt gatttaaagt tattacctta
    34801 acttcttcac ctggctttaa gtcttttatc tccttatttg atacctttgg aactatcata
    34861 ctttttactg ttggctgaag acttcccata gagctagtca attcttttct aagttcttct
    34921 atcttacgat tcttttcttt tgaagctctt tcttgttcaa gacttctaag ttcttttatt
    34981 ataatatcta cttcttcttt agcttgtctt ataattgaaa atgcttctga tttagctttt
    35041 tcaatcatct tatctctttg actgaccaat ttttcaagtt tttcgtcata ctctactttc
    35101 agtttttcaa tctcttcttt taatctttca gcttcttctc tatcttctac tgctttgatt
    35161 ctattctttt caacattctg taacacatct tctaaagcta tgttttctgt atttatatat
    35221 tctttagcac gtgaaatcac ataatcactt agcccaagtt ttctagaaat ctcaaacgca
    35281 tttgattttc cgggaacacc tataagtaac ttatatgttg ggcttaaagt ttctatatca
    35341 aattcaactg ctgcgttttc tacacctggt ttagtaagag cataattttt aagctcacta
    35401 tagtgagtgg tagctataca tttagctcct acagaattta tatcttctaa tactgctatt
    35461 gcaagggcag ctccttcaac agggtctgtt cctgctccta actcgtcaaa tataactaaa
    35521 gagtctgctg ttacgttttg taatatagat actatattcg tcatatgaga tgaaaatgtt
    35581 gatagactct gttcaatact ctgctcatct cctatatctg caaatacatt atcatataca
    35641 cacatactac ttccataatc agcaggtata tgaagtccac tttgtgtcat aagtgcaaat
    35701 agacctacag tttttattgt aacagtctta ccacctgtgt ttggacctgt tatcaccaat
    35761 gtatggaagt ctcttcctaa ataaatggta tttgctacta ccttcttttt atccagcagt
    35821 ggatgtcttc cattttttat gtttaaatat ttatcctcat ttaaagttgg ttctattcct
    35881 ctcatctgta tagaaagttt tccctttgaa aatgcaaagt ccaatcttcc aagtatctct
    35941 tgattagaga ttaaatcttc gctcacttct ccaaccatag cagaaagctc tgataaaatt
    36001 ctttctattt cttcttgctc acctagtttt aactgtctca attcattgtt catctctact
    36061 atagtcattg gctctataaa aagtgtcgca ccagaagatg actggtcatg cactatacca
    36121 gctacttgcg acctatattc agattttaca ggtactacaa atctatcacc tcttagagaa
    36181 attattgcat cttgtaagta cttttgataa gtagttgaac ttattattga atttaatttt
    36241 gacctaatgg attgattttt ttgggctatt cttcttctga tatctctaag tgtacttgaa
    36301 gcattgtcag atatttctac ttcactaatt atagcattgt atatctggtc ttctatgtcc
    36361 ttgtatacat atagagaatt tgatagagat tgtattattg gataattaaa atcttcttca
    36421 tcactactag ataagctatt tcttaatctt ctagccactc ttaaggtatc tgctatcata
    36481 atcagacttc ctgggtcaag agaagcacct atataagctc tctttacctt atcttctata
    36541 tcatgaatac cttccaaccc tactgaacct ctctttataa taattgattg agcttcacta
    36601 gtttcttcta gcatagattt cacttctaca aaatcagtat ttggtactaa attttctata
    36661 tattttagac ctaaagaaga tgaggctttc ttctttaaca aatctattat tttattgtat
    36721 tctagaactc ttaacgattt ctcattcatt ttctatattc accatcctcg atattttaac
    36781 ttttttattt aattttaaaa atgtatgttt tcaagcattt ccaataaaaa ctgcaagttt
    36841 tatttctttt tcatttctaa aattattata ttttttctct tttagatata ttttacacat
    36901 attttgtatt atactatgta tgtagctatc tatatattat acataaacat gctttatttg
    36961 taatataatc tgttaaaaaa tcttctttaa tatttttaaa ggagtttatt ttagcttata
    37021 aaaaaaaata gctacttata tagataaatt aaggaggaac atgagtcatg ggaaacgtat
    37081 tagaaggatt aaaaccagaa agtgttttta aaaactttga gaaaatatct caaattccta
    37141 gaggttctgg aaatgaaaaa ggcataagtg attttttact tagctttggt aaaaatttag
    37201 gtcttgaaac tattcaggat gaaagcttaa acataataat aagaaagcct gctacaaaag
    37261 gatatgaaaa ttgtccaggt gtagttcttc aaggacatat ggatatggtt tgcgaaaaag
    37321 aaaagaatgt tgaacatgat tttcttaaag accctataaa attaagaatt gatggtgaca
    37381 tgatatatgc tactggaact actctaggag ctgacaatgg tatagctgtt gctatgggta
    37441 tggctatatt agaagataat actttagagc atcctgcact agaagtttta gttactgtta
    37501 atgaagaaga tggtatgaat ggtgcagatg cactagaccc tagcttaatt aaaggtcaat
    37561 atatcctaaa tatggactcc gaggaggaag gatacttatt agttagctgt gctggtggta
    37621 aaacttgtgt tgttagttta cctgttgaat acaaggaagt taaaggtgac aaacaaggat
    37681 tattagttga ggttactgga ctattaggtg gacactctgg tatggaaatt gttttacaaa
    37741 gagcaaatgc taacaaagca atagctagag tattaagtgt attgaatgtt gattatgaat
    37801 tagcttctgt tgatggtggt actaagcaca atgctatacc tcgtgaagct aaatgtgtaa
    37861 tagctgttaa taaagctgat gttgaatctg ctaaaaaaca aataaatgac attttaactg
    37921 catttaaaca tgagtttact acttctgacc ctggcatgac ttactcagta gctgaaactt
    37981 ctgtagacaa agttcttact aaagattgta aagaaaaagt tgttcaaatg tcttgcttaa
    38041 ctcctcatgg agttcaatca gtaagtcttg atatagaagg attagttgaa agttctacta
    38101 acttcgctat aatcgaaact aaagaatcta ctattgaatt tttaacttct gttagaagtt
    38161 ctgttatgtc tattagagat gaaatcgctg atagaataag acttttggct caagctttag
    38221 gtgctaatta cgaccttata gctcaatacc cagcttggga atttaaaaaa ggctcaaaat
    38281 tagaaaaaat atgcagtgaa acttatgaga aattaactgg taaagttcct actgttatgg
    38341 ctctacatgc tggtcttgag tgtggattat tacttgataa acttcctcat gctgaagcta
    38401 tctctatagg accagatatg tttgatgttc atactccaaa tgaacatgtt agtatccctt
    38461 ctgtagctaa tgtttgggat tatgttattg aaatattaaa atctatgaac caatattaaa
    38521 tttaagtttt gattctaagt agattttaat tttaagtaat ctatataaaa ttaactctgt
    38581 aaattaatac tacggttagt ataatattta ctttcaatat taacttatag aaataaagcc
    38641 taatgataga taataacatg tatcattagg ctttattgta atttaaatat tattaagctt
    38701 tattctgttt taaaattaat tcttatacta ttttaaatct atttcttcct tgtctcttag
    38761 aatcataaag agcattatct gctatttgat acagttcatc atactcttga ccaggctttg
    38821 ctataactac ccccatacta atagataaac tttctccatt tggcaactta atgagatgaa
    38881 tatgattgta tacatcatta catttattaa taagttcttc cttatttatt atatcattga
    38941 tataaacact aaattcatct ccacccaaac gacctactat atcatttttt gcaaaacttt
    39001 catcaagtgc ctttgcaaca tcaataagta ctttatctcc taacaagtga ccataagtat
    39061 cattaatcga tttaaaataa tcaatatcta taatcaataa agcaccattt ttttcagttg
    39121 ataaattcaa caaattttgt gaagttaatt ttctaatata tgctgcatta taaatattgg
    39181 ttaaactatc cctttgagcc ttatctaaca atctattctt ttcctctttt tcagaatcaa
    39241 tattacatat tttcccgatt acatacattg gtatttttct atcattataa attatttttg
    39301 aagtaactct aatccaatga aaagtatcat caagcataat agaatacatt tcagttgttc
    39361 cattttcttg tgattttatt acatcaaaaa actttttatt atattctttc cttattttat
    39421 ctctttcatt ttctatattg ccattttcac tttccttatc atttttatcc aaaccataat
    39481 aagtctcaat acccttttct ggcatagaaa ttatcaattt atcttgctta taatcatact
    39541 caaaaaagta ttcacttgtc atttcataca actgagtatg ttttttgagt tctaaagata
    39601 tcttctcgtt catacttgta cgagtatgta aaccaaatgc cataaatcca ataatacaaa
    39661 ttaataaact ggctacaata aaaatagtct caattggatt ctgttgcata atataaagca
    39721 acgagaaatc ttgtctatat gtagtatttt ggtagacaat tgattgcatt ttttctgatg
    39781 aaatacttat aatagcttta ttaaatatat tcaataattc ttgtttatct tttttaatta
    39841 taccaaaaca aatactcttt gctttatagg tttgaggaac tagacgcaca tttttatact
    39901 taggttgatt catatagtac tgcatagaat aaccttcgcc ataagtgtaa tctgcatagc
    39961 ctttattaac agcttctata cactcttcta aattatcata ccaaactata ttctcttcaa
    40021 actgaccata ataattaata cctttggcta gagctacacg ttttccatct aaactattct
    40081 catttaattt attattaatc atcatagtat attgagtaga aagaaaaggc cgagacatag
    40141 ctatattatg ttcacgagag tattcataat cataaatcat tcctgctaca atatcaattt
    40201 cacgcttttt agtcatgttt tctaaatctt cttgagattt tacaggcaca agttcaaatt
    40261 ctaagcctgt tttatcacat atatgattta acaaatcaat tgatatacct cttaattcac
    40321 ctgtatcttt atctatattt tgaaatggtg gtttgtttaa aagtactcct acttttaatt
    40381 tttctgtctc tttaatgtaa ttagtttctt catcagataa aaacatttga ttattctctg
    40441 gtgtaaagta tttttcatat aaagtagttg aaaaatatgg gtctttctgt tcaatattat
    40501 taattgctga gttaagcttt tgagttaaat ttgtactacc tttaggagta atgaaataaa
    40561 atggttttga tgaaaatttt gctacagtac gtacaccttc tattgggttc atactcacat
    40621 taagaagtac atctgcttta ccactcttta gtgcttctag ttgctcatca tttgttttac
    40681 aatataccaa ttcaggtgac ataagattca ttttacaata ctcatctaat tctttaagtc
    40741 tagtatcaga attttctatt acagcaatac ggattttttt cacatcttgt gaactcatta
    40801 tgttatcaat atctgtattt tcatataatg cttgtagtac tgtatttgct actccataat
    40861 tattccctgc ataattatat attttttgca ttggttcatt gtataacata cctcccatta
    40921 tatcaatttc accattttct aacatcttca ttaattttgt aatagattca tttatctctc
    40981 caggaacttg aacaaattca taattccatc cagtatactg tgcaatttct agtaaatatt
    41041 catatgtata cccaaaatag ttaccttgct catctatttc agttaaacct ttttgaatag
    41101 gataagcaac acgaatggta cgattatcat tacctatagc gttgatacca aaagaaaata
    41161 tgcaagaagt gaatatagat gtaattacaa taattaataa aagtaatttt tttaaattca
    41221 ctacacaatc ctccatatag ttaagattta cagtataaat tactgcataa acttgtagac
    41281 atgtaaaaat agattagtat ataaaaggat atataaatct acttagaaat ttaaatatcc
    41341 ttaatatata caatatattt aaattataac tcatttatat gtctattaca acaaatttac
    41401 cctgctaaat acctactcta tatcccataa ttttacaagt tgtagtgaag tttaagttct
    41461 atctcacctc aaatttagca gtgtttctat ttacagttct tttatatttc acatctggat
    41521 aacccaatat aaatgaagtg ataactgctt cgttctcgct cattcctaac agttctttta
    41581 tctttggctc tacatttgat gccataacaa aaaatccatt ataacaaatt cctaatcctt
    41641 gagaataggc cataagttcc atattagatg cagccaatcc tccatctaca taagcacttc
    41701 cacctagaga catatcacct acaactacca taagtagtgg ggcatcataa aataaagaat
    41761 catttccgtt ctctttgtat cttttgtaca ttttcttaaa tgtattttta tagatagaac
    41821 gtacagggtc attgtcatca gtatctaatg ccaaattata aagaccttgt attgccagtt
    41881 ctttcacttc ttttagcttt tcttttacta atatgtatct tataggctgt ctattaccac
    41941 ctgttggtgt gtatctacca gcttccacta tgttttttat tttttcttct tctacctcta
    42001 tatctttata ttgtcttata cttcttctaa atttaataaa attcaataac ttttcgggtt
    42061 ctaattcaaa actttcacga ttgtattcta ttacttcatt tttatcaaat ccttgcattt
    42121 ctattgcatc tgttgggcaa attgccatac agtgaccaca gttaatacag agaatatttt
    42181 taacctttgc tttatcatcc ttaactttta ttgcatttac aagacagtct gattcacaca
    42241 tcccacagcc tacacatttt tctaaattta tgtctatcat tccaaattat tcctcctttt
    42301 aaaaaattta ctttagtata aaacaaatag ttgtctaagt caatttttat ttttttaaca
    42361 atatactagc cttaactaca ctttaatgga gaaaaatata cataaattgg aacttaaatt
    42421 agaacttatt caaagtaaaa gataaaagaa gtagatgtct aaaagtgaac tttttagtta
    42481 attagatatt ctttattgta tgaaatcaag tggatattta tcatttcaac aataaaaaag
    42541 gcttacctct attttggata tgtccctgtc aagtagacag actaaaaaaa ggctatcttt
    42601 atgcactgag atagcccctt ttcccaacta ctattgttta tcttaatatt agtcaataaa
    42661 tttaatttgc tataacctgt aagttacatt ttaatttgat gtaactttaa attacatttt
    42721 taagtaatat ctttaaatta tattcttaaa atatatcatt aaactacatt ttaattaatc
    42781 taaaaactat atcttaatta atctaagaaa cttacattat aatcttttag tttcccatta
    42841 tcatatacaa tctttgcact aaagatattt ccagtcttag cccaatttaa gaacagcctg
    42901 tcaccttccc acatatttaa atcgaatatt ttatcattat caacccaaat taaatttcct
    42961 tctttacaat catttaaatc tttttcagat aattcacctt caaactcatc tgagaaatac
    43021 aaaaacatat accaatcttc ttcatcttta aattttggaa aagttatcaa ccctttatat
    43081 gacaatgatt taagagtaag accagtctct tcttttactt ctctaattac acattcttct
    43141 ggagtctctc cttgctcaaa ctttcctcca actcctacat acttaccttc atgtatatca
    43201 tctttctttt tatttctgta caacatcaaa gtcttgtcat ctttttctat ataacaaata
    43261 gttgtaagtt tcatgtatct cgccttgctt tctcttttat attctcaact aattattttc
    43321 agcactttca agcacatcac ttatattctt cattgcatta agagaaagct ctgcaaattc
    43381 atcaaatgga atccctatat cttctatact tctcattgca tctctattta catttgaagc
    43441 aaatctcgtt tctttcattc ttttaataac agacttagtc tttacactgg taagtttttt
    43501 atctggataa actaaagcta tagcagttat aaacccagtt atagggtctg ctgcatatat
    43561 tgctttttcc attaggctag ttcttgtaac tcccattcca tcatgatgac caagtattgc
    43621 tctatacatt tcttcgttac cgaagttttc ttttttaagc agttctactg ttgttgtagc
    43681 atgtccttca catatttctc ctggctttct tccaactata tctgaatcca aatcatgtaa
    43741 aagacctgcc actgcccact cttcttcttt ttctggctct agtcttttgg ctaattctct
    43801 cataacagct tcaacagcat aacaatgttt tctcattcta tcagtttcta aatacttaaa
    43861 taaaatttct aaagcttctt ctctcatttt caccaatccc cctgtttttt cacatatatg
    43921 atatttttat atattaatac acataaattc aagcttttat atatattcat gtatattttt
    43981 catgttatat ctattctaca cctttatagt agtgtcctgc aactgttcca atttcatcta
    44041 atttcttata caagtctgta gccttttttc caagtttata attattactc agtacctcaa
    44101 tgtgagcttc aataaccttt ttaaccatct cactatcttc aactgtaaaa tctatcctaa
    44161 atacatctat tccaatttcc tctaactcat atagattgtc caacatacaa gtaaccttcg
    44221 aattgtaaat tgtacttcta cagaatatat cttgtgttat cctataactt gcatccgtaa
    44281 agtctcttaa agcataacta ctttccttac atttagcaac tctcttgtcc tttttacaat
    44341 ctctaacaac tactcccata ggacagtatt ctgtaaccat cataggagca tatccatata
    44401 ttactgcttc aatttctaca tctgtatatt ttaaagtttg tttaatttca tttaagttta
    44461 gttcttgtga caaacaaact gtacttgctc cctcttcttt gaagtgattt atactttcac
    44521 tattgaatac atttaagtag caatctatat ttaagtcttt tttcttaaag tattccatag
    44581 agccccagtt accaatttga accttattaa ttccttttat attatccaat atctcaaatt
    44641 gcttgtactc tttatttcta acaatccttg gtgctgaata gattaatttt ttgtcagtga
    44701 ctgctgtatt agagtggttc ttattcaaag tatctatttt acttgtattt gacaaagtat
    44761 ctatgttatt tgtatttgaa cccattagtt ctttaggagt acctaaagac gaagaagtat
    44821 ctaaatccat agaaatactt gaatctatag aaatatttga atctatagag atacttgaga
    44881 ccatttgtaa agcttttgat aatgtattta tatcttcata atagattaaa tctacattat
    44941 aatttaatac tgcttctaat tgctccaagt tcttaacttt aactctaatc ttaggagctg
    45001 tatcaagttt tttctgttta tttactctat ttggctggta ttttaatcta ttcttcttaa
    45061 attttctatc ttttatcttt attctttctt catctaaaag ctcaatacaa tcccttctca
    45121 tttgatttaa cacacttata ggcatactta caccttcatc caggtcaatt tctatctctt
    45181 ttaacttata tggtgtattt ccaagctttt gaatttgtgt ttctattttt tctttactta
    45241 aagccacctt tatagctttt tcaactattt tgtctccttc taccaccgat ttatttccac
    45301 aaaaatcact tataaaaagc tctggctttt tacctagttt aatactcact tttgcacgta
    45361 tattagtttt tatgaactct ttatcttgct gaaatgtagc ttgtactcta tccattaatt
    45421 cgctgtcaga agtcttatat ataagctgat ttctctttgc ttctccaaca aaatctaatt
    45481 ctatcaaatc ccctttatgt gctattgtag ttatctctcc atcctttatt attcttccaa
    45541 tagtacctcc acctaagtta attccatcac ctttcttaag cgtattttct agcattactc
    45601 taagtctttt gttctttcta ttataatcta aaacttttcc aatatatagc ccttgattat
    45661 taggtctatt agagttcatt atatccttac caacttcacc taatatatat ccttttgtaa
    45721 actttctatt aaatatggta tacaaattat ttattatttc ctcagaaata cttatttttt
    45781 tcttatctac aaattcatta atcgcttctc tatatcctga aaccacagta gctacatact
    45841 ctggcttttt cattctacct tcaattttta aagataatac acctgcttca attattttat
    45901 ccaaatcttc tatagtactt aaatctctag gactcaacaa ataatctcca ttacttttta
    45961 ctacttctcc tgtatttata tctatcaact catacttttg tctacatggc tgtgcacacc
    46021 tacctctatt tccagaccta ttgcctatca tactactcat aaggcattga cccgagtaac
    46081 atacacataa agctccatgt acaaaaactt ctatatctac agttgtattt tcacaaatcc
    46141 attttatctc atcaacattt aattctcttg ctaaaacaac tctatcaaac cctacactct
    46201 gaaggtattt tacatcttct aaggaatgtg ctaccatttg agtgcttgca tgtagttcaa
    46261 agtcaggtaa tagttttttt ataagtcttg ccattcctat atcctgtact attaaagcat
    46321 caatatctat atcgtataaa aattttacat actctacaaa atcttctatt tcatactgtt
    46381 ttatcaatgt atttacagta acaaagactt gcactcccct tatatgagag tatttaacag
    46441 cctcaatcag ctcatttcta tcaaagttat ttgcagatgc tctagcacta aagtctttac
    46501 cacctaaata cacagcattt gctccgtttt gaactgctgc ttttaatgaa tcaaaagagc
    46561 ctactggtgc aagtaattct atttttttca tatttttctc ctctatttat acttgttagg
    46621 ttaatataat tatatctata acttaaatat ttgtattaaa tttatatatt tcttactata
    46681 ttttaaattc ttcaatcaat ttatatatta gttatttata tttggttcat ttgagttaac
    46741 atatacatta tttactatat ttagtatatt tctattaact tatatattga ttactgtatt
    46801 ttaaattctt caattaattt atatactgct tactaaattt aaaacatttt aattaatttg
    46861 tacactgttt actatacttg aaatacttct attaatctat atattgccta ctatacttaa
    46921 gacattttaa ttaatttata cactgtttac tatacttgaa atacttctat taatctatat
    46981 attgcctact atacttaaga cattttaatt aatttataca ctgcttacta tacttaaaat
    47041 acttctatta acttatacat tgcttactat attttaaatt ctttaattaa tctatgcaat
    47101 atttgctata tataaagtat atcaaataac agcttcattt ttttgtaaat ttacagaaag
    47161 ttaattttca ttaaatgaat catctttatt gataaacata attccaaaga ccactgccaa
    47221 aataaaaaaa catattagta ttgatttttc cacgaaaatt tttgtaaaaa aagttcctaa
    47281 aatagctcca gatataaaga atataataac agcataatac tgtaaactct tttttatcat
    47341 aaaaatattc ttagtatgta tataatgaaa taaagtttct gtagcacttc ttaaatttcc
    47401 agtacacatt gtcgttgcaa aactattccc atttactttt ctaaaactct ctacttgcat
    47461 tgaacaaata aaagaaatag caacatttac aaacatatta aatcttcctc taggaataaa
    47521 tgaaacaata agtattgtaa tcatttcaat tataatgata acctgacgcc aatgaatctt
    47581 tgtcttatta tttgtaaaat actttcttat cttctcggct ataaatacac ctaacataaa
    47641 tgccaaaata ggaatcaaat aataaaatga ttcctttaca ttaccttctg ctatattaag
    47701 cccaaacaaa actatatttc cagtttgtgc attagcaaag actttacctc ttaccacata
    47761 agtataaata tccaaaaatc ctcctgttat tgccaacaaa gaacataaaa agaatgattc
    47821 agacatctga ctcttgcttg tcatatataa acctctcgct ttctaatgat agatacaaaa
    47881 tcaaaatcat tttagattct atttttcatt gatataaaaa aattatttat atttaaatat
    47941 tgtctatatt tatacatcat ttatatttat acattaaaag aagctgtctc aaaaaacaag
    48001 agacagcaaa tcttttagtt tattccacat tttttgcatc attatttaac cctgtacatt
    48061 ttaactgtaa atcataagct ttgttttgaa actcagaagc taagttttca gctatagtag
    48121 ctctttcatt tgcttcttca agttgagtac ttaactcctc aatcttgttt ttataagcat
    48181 ctaactctgc tctagaacct tctgtcgtat tactaagttc gaatatttct tgctttttat
    48241 tttctataat ctcaattaac tctttcatct tagtttcagc ttcacgattt tctgcttctt
    48301 tttctgctat cgtaagcttt aaagacttga tttctaattg aagctcttca ttagatgctc
    48361 caaccttctt actcaattct tcattagctt taatcaactt ctcattctca tctgaacatt
    48421 caaataaaag gtctgatata tttaatgcag ttaatatcgc agcaactgat aagctcaatt
    48481 tcgggttttg acgtgttatt ttatccattt ctttgtctac aaaatcagca atactaatca
    48541 taaacttctc tgatttgtct ccaaccattg gatattcagc gccatggatt ttaaccatta
    48601 ctttgttcat cctaacctcc cccgacatta gttgttaatt tgatcttaag ttagcattta
    48661 atttttcgct taattcactt aatatcttat catgcacctt agctacatct tcatctgtta
    48721 gagttttatc tttacttcta taagttattg aatatgcaat tgacttgtat ccagcttcta
    48781 tttgagaacc tttgtaaaca tcaaataact tgtagctttc tactaatcct tgaccatttt
    48841 cctttattat atcttctatt tgttttacaa atacatcatc tttaacaata agagctatat
    48901 ctcttgatgt agatggatat tttggtaatg gcacataatt ctttgttcta tcagaattat
    48961 caaatacaaa gtcaatatct atttcagcga cataaactct ttgacctaga ttgtagtttt
    49021 ctataacatc tggatgcaat tcacctaatg tacctacata tttattatta tacactattt
    49081 tagcacatct accagggtgg aaggtagtat tattatcttg aggttcaatt tcataacctt
    49141 taaatccgac atttacaagt atagtttcta tagttccttt tagtgcaaag aagtcaacat
    49201 ctttaccata catacctaca cacattctat tttcttcctt aggtaattct gaatcttgtg
    49261 ggatgaatat atgtccacac tcaaatgcag atacttcttc tattttatga gatacattag
    49321 ttgataaaac atctaacata tttggtatta aagtagttct cattacagaa gtttcttctc
    49381 ctaatgggtt aagtaattta acaaaatttc tctttgcatt accttctggt actcttatct
    49441 tatctactcc cttagggctt acaaatgaat atgttaatat ttcatttaat cctacagcag
    49501 ttgaattatc ttttaaagca tccatgaatt tttgttttga agttttaatt cctgctgtcg
    49561 tatttccttc taattgtaca gaaggtatat tctcaaatcc ataaattcta gctatttctt
    49621 cccatacatc agcttcttgt tccatatctg ttctaaagct tggtacatct atttctaatt
    49681 tatcattagc taccaaatta catttaaact ctaatgattc taaaattcct acaaactgct
    49741 ccattggtac atctacacct aatagatggt taattctttg aggatttact accaattttt
    49801 taggttctgg tttatttgga tatacatcta caacaccttt taaaactttt cctgctccta
    49861 acatttcaac aagctgtgca gctctatttg ctgctatctc tgcaagatta gggtctaagt
    49921 ctttttcatt tcttgaagat gcttctgacc taataccaac ttttttagct gtcattctta
    49981 tgttttctgg tttgaaattg gcactttcaa aaagtacagt cttcgtatta gaagttattt
    50041 ctgagttagc accacccatt acaccagcta aaccaagtga tttttctcca tttgttataa
    50101 ctagcatatc tttgtctaat gttctctcaa catcatctaa tgttacaagt ttctctccat
    50161 ctttagcatt tcttactact attcttccag tctctacttg atttatatca aaagcatgaa
    50221 gtggttgacc aagctctaac attacgaagt ttgttatatc gactatgtta cttataggtc
    50281 ttactcctgc ttctgtaagt cttctttgca tccaatatgg agaaggttct atttttacat
    50341 ctgtaaccat tctagcaaca tatcttctac ataaatctgg attatctatc tcaactttga
    50401 aatctatctc ttcgtcactt tcttttactt cgatttcagg atattttact tttgttccta
    50461 tagttgcagc tgcttctcta gctataccca tcatgcattt acaatcaggt ctgtttgaag
    50521 ttaattcaaa atctattaaa gcatctttta atcctaaaac atcttttata tcttttccta
    50581 attcataaga atcttcgtgg tctaaaatat atataccacc tcttttatac tcctcaatgt
    50641 aatgttcatc tatacctagt tcagctgctg aacacatcat accatctgat aattcacctc
    50701 tgaaatcagt ctgtttgatt tcaactcctc caggtaactt agaaccattt acagctactg
    50761 gaatataatc tccttctgat atatttgtag ctcctgtaac tatttgaaca actttatctc
    50821 caatatctac tttggttaca accaacttat cagcatttgg atgttgtttt atttctaaaa
    50881 tctttccaac caatatattt tctatttctt caccataata atctattgtt tcaactttag
    50941 ttcctgtcat tgtcatttta tcagcgaact cttttacatc catgtctata tcaacatagt
    51001 ctctaagcca ttttaaagat actaacatct acataccctc ctaattaaaa ttgatttaag
    51061 aatctcatat cattttcgaa taataatcta atatcatcta tttcgtattt aagcattgca
    51121 agtctttcaa ccccaactcc aaatgcaaat ccactgtaaa cttctgggtc tattccacaa
    51181 tttctaagca catttggatg aaccatacct gaacctaata tttctatcca accttcatat
    51241 ttacacattg ggcaaccttt accaccacat ttgaaacaag taacatcaac ctctgcactt
    51301 ggttctgtaa atgggaagtt gtgaggtcta aacttagttt tgatatcaga accaaacaat
    51361 ttttcaacga agatatccat agttccttta aattctgcca tagtaacatc ttttccaaca
    51421 acaagccctt ctatctgatg gaacattggt gagtgtgtag catctggcga gtctgaccta
    51481 aaacatctac ctggtgcaat tacttttatt ggtaactctt gacttctcat agttcttact
    51541 tgaactggag atgtttgagt tctaagtaat accccatcat tgatatagaa tgtatcgctc
    51601 atatctcttg atggatggtc tttaggagcg tttaatgcgt caaagttgtt ttcaacagtc
    51661 tcaacttctg gcccttctgc tatagagaat cccattccga taaatatatc tgttacttca
    51721 tctataattt gagttattgg atgcttcttc ccaaccttta aaacttttcc tggttgagta
    51781 acatctatca cttcctcttt taattttctt tctttttcaa tagcatttat ttcttctttt
    51841 ttagaattta tgctaagttc aatgttttct cttacctcat tggcaacctt accaactact
    51901 ggtctttctt cagcagataa tttacccatt tctttaagta tggcagttat ctcacctttt
    51961 tttcctaagt acttaactct taaactttct acactttcta tgctttgtgc ttcttttatt
    52021 tcagccaaag ctgcttcacg taaagcaagt aatttttctt gcacacatat caccctttcg
    52081 tttttttgta taaaaaaagc ccctcaatcc ctgtctaggg acgaaaggct atagtttcgc
    52141 ggtaccaccc tagtagttta acaatttaaa aatcatcaaa ccacctctaa caaacttaac
    52201 gcgtttttga cgtcaaagcc tactgtaaaa tttcagctct gttactccag agggaacttc
    52261 tcatgacctc ttagaaaaca ctcccagcct aggtgtttat ctctttacta agtttaatca
    52321 aaatactttc ctctttcatt gcatttacaa tattatattg tttattttag tattatatca
    52381 tttccattca aattatgtga ctatttttaa tatatatcca atacataatt aatttaatta
    52441 ttttttatat aatatacatc taatgcataa ttaac
//
LOCUS       DACULQ010000003        50195 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.003, whole genome shotgun sequence.
ACCESSION   DACULQ010000003 DACULQ010000000
VERSION     DACULQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.003"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            121..990
                     /locus_tag="JA154_00650"
     CDS             121..990
                     /locus_tag="JA154_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HAT7994365.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            1216..2080
                     /locus_tag="JA154_00655"
                     /pseudo
     CDS             1216..2080
                     /locus_tag="JA154_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            2272..2469
                     /locus_tag="JA154_00660"
     CDS             2272..2469
                     /locus_tag="JA154_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7994366.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            2472..2915
                     /locus_tag="JA154_00665"
     CDS             2472..2915
                     /locus_tag="JA154_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994367.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(3102..3189)
                     /locus_tag="JA154_00670"
     tRNA            complement(3102..3189)
                     /locus_tag="JA154_00670"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3153..3155),aa:Leu,seq:caa)
     gene            3341..5989
                     /locus_tag="JA154_00675"
     CDS             3341..5989
                     /locus_tag="JA154_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAT7994368.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKDYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            5998..6600
                     /locus_tag="JA154_00680"
     CDS             5998..6600
                     /locus_tag="JA154_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAT7994369.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            6593..7147
                     /locus_tag="JA154_00685"
     CDS             6593..7147
                     /locus_tag="JA154_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAT7994370.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            7147..8862
                     /locus_tag="JA154_00690"
     CDS             7147..8862
                     /locus_tag="JA154_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7994371.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNITSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            8935..9147
                     /locus_tag="JA154_00695"
     CDS             8935..9147
                     /locus_tag="JA154_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain protein"
                     /protein_id="HAT7994372.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            9348..9947
                     /locus_tag="JA154_00700"
     CDS             9348..9947
                     /locus_tag="JA154_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994373.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            10175..10546
                     /locus_tag="JA154_00705"
     CDS             10175..10546
                     /locus_tag="JA154_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAT7994374.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            10657..11952
                     /locus_tag="JA154_00710"
     CDS             10657..11952
                     /locus_tag="JA154_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding protein SH3"
                     /protein_id="HAT7994375.1"
                     /translation="MLGGVIVVKKAIAALGIGAVAVSVSSINASALEKGTVTASALNI
                     RSGPSSDCDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSS
                     QNNPTSSGTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIK
                     LSNGVTGWASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSL
                     NVRSGPGTSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKE
                     KPNSSSNQNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDC
                     SGFTQYVFKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYV
                     GNSKFIHCSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            12063..13007
                     /locus_tag="JA154_00715"
     CDS             12063..13007
                     /locus_tag="JA154_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994376.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            13223..14560
                     /locus_tag="JA154_00720"
     CDS             13223..14560
                     /locus_tag="JA154_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAT7994377.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            14583..15560
                     /locus_tag="JA154_00725"
     CDS             14583..15560
                     /locus_tag="JA154_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+-transporting NADH:ubiquinone oxidoreductase
                     subunit D"
                     /protein_id="HAT7994378.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADISSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            15560..16129
                     /locus_tag="JA154_00730"
     CDS             15560..16129
                     /locus_tag="JA154_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter"
                     /protein_id="HAT7994379.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            16142..16735
                     /locus_tag="JA154_00735"
     CDS             16142..16735
                     /locus_tag="JA154_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HAT7994380.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            16750..17325
                     /locus_tag="JA154_00740"
     CDS             16750..17325
                     /locus_tag="JA154_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAT7994381.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            17335..18312
                     /locus_tag="JA154_00745"
     CDS             17335..18312
                     /locus_tag="JA154_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfB"
                     /protein_id="HAT7994382.1"
                     /translation="MKMVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGV
                     LPGANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVA
                     KVICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIV
                     DGIAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIG
                     CGMCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQEL
                     CVGCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            18532..19116
                     /locus_tag="JA154_00750"
     CDS             18532..19116
                     /locus_tag="JA154_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087635.1"
                     /note="Maf; overexpression in Bacillus subtilis inhibits
                     septation in the dividing cell; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAT7994383.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            19113..19820
                     /locus_tag="JA154_00755"
     CDS             19113..19820
                     /locus_tag="JA154_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994384.1"
                     /translation="MIYLKKSFNSTIKVKEMAPEERPREKMLAKGVKSLSNAELLAIL
                     LRTGNKNKNAIELANYIINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGS
                     RVASFKPIKYKIRNPWDIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSS
                     SLVHPREVFKEAIRRSASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDH
                     IIIGDGLYFSFKENMII"
     gene            19890..20951
                     /locus_tag="JA154_00760"
     CDS             19890..20951
                     /locus_tag="JA154_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087637.1"
                     /note="functions in MreBCD complex in some organisms;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAT7994385.1"
                     /translation="MAKEKKKEKKGFFSFNKMTKDMGIDLGTANTLVYIKGQGIVVRE
                     PSVVAIRDDSKEVLAVGEEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQK
                     AADKKGVVSPRIAICVPFGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVE
                     EPEGNMVVDIGGGTSEIAVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERT
                     AENVKINIGSTFKDDEEINMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIK
                     STLERTPPELASDIMENGIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTG
                     KSVEDQEIFEKVLMMNTKN"
     gene            20955..21854
                     /locus_tag="JA154_00765"
     CDS             20955..21854
                     /locus_tag="JA154_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAT7994386.1"
                     /translation="MVMALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSS
                     GSPVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNM
                     IALNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGV
                     KKESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDN
                     KDSYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYV
                     VVKPYVDFKNINDVVVIEPRNIG"
     gene            21869..22357
                     /locus_tag="JA154_00770"
     CDS             21869..22357
                     /locus_tag="JA154_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAT7994387.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            22369..25347
                     /locus_tag="JA154_00775"
     CDS             22369..25347
                     /locus_tag="JA154_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAT7994388.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            25445..26128
                     /locus_tag="JA154_00780"
     CDS             25445..26128
                     /locus_tag="JA154_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAT7994389.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            26152..26949
                     /locus_tag="JA154_00785"
     CDS             26152..26949
                     /locus_tag="JA154_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087642.1"
                     /note="ATPase; with MinC inhibits cell division by
                     blocking formation of the polar Z ring septums; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAT7994390.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            26967..27251
                     /locus_tag="JA154_00790"
     CDS             26967..27251
                     /locus_tag="JA154_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087643.1"
                     /note="works in conjunction with MinC and MinD to enable
                     cell division at the midpoint of the long axis of the
                     cell; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAT7994391.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            27336..28466
                     /locus_tag="JA154_00795"
     CDS             27336..28466
                     /locus_tag="JA154_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HAT7994392.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            28507..28920
                     /locus_tag="JA154_00800"
     CDS             28507..28920
                     /locus_tag="JA154_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAT7994393.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            29405..29734
                     /locus_tag="JA154_00805"
     CDS             29405..29734
                     /locus_tag="JA154_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAT7994394.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            29738..30529
                     /locus_tag="JA154_00810"
     CDS             29738..30529
                     /locus_tag="JA154_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994395.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            31093..32175
                     /locus_tag="JA154_00815"
     CDS             31093..32175
                     /locus_tag="JA154_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAT7994396.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(32387..33580)
                     /locus_tag="JA154_00820"
     CDS             complement(32387..33580)
                     /locus_tag="JA154_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HAT7994397.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            33899..35743
                     /locus_tag="JA154_00825"
     CDS             33899..35743
                     /locus_tag="JA154_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HAT7994398.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            35745..36446
                     /locus_tag="JA154_00830"
     CDS             35745..36446
                     /locus_tag="JA154_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT7994399.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            36464..37885
                     /locus_tag="JA154_00835"
     CDS             36464..37885
                     /locus_tag="JA154_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HAT7994400.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            37986..38297
                     /locus_tag="JA154_00840"
     CDS             37986..38297
                     /locus_tag="JA154_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAT7994401.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            38312..38629
                     /locus_tag="JA154_00845"
     CDS             38312..38629
                     /locus_tag="JA154_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAT7994402.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            38643..38933
                     /locus_tag="JA154_00850"
     CDS             38643..38933
                     /locus_tag="JA154_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAT7994403.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            39044..40321
                     /locus_tag="JA154_00855"
     CDS             39044..40321
                     /locus_tag="JA154_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase CgtA"
                     /protein_id="HAT7994404.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            40373..40741
                     /locus_tag="JA154_00860"
     CDS             40373..40741
                     /locus_tag="JA154_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAT7994405.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     regulatory      40833..40939
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            41042..42247
                     /locus_tag="JA154_00865"
     CDS             41042..42247
                     /locus_tag="JA154_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HAT7994406.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     gene            complement(42448..43020)
                     /locus_tag="JA154_00870"
     CDS             complement(42448..43020)
                     /locus_tag="JA154_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAT7994407.1"
                     /translation="MKMATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALA
                     LMLEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYN
                     LKSNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKEL
                     LNHSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(43294..44553)
                     /locus_tag="JA154_00875"
     CDS             complement(43294..44553)
                     /locus_tag="JA154_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HAT7994408.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            44678..45391
                     /locus_tag="JA154_00880"
     CDS             44678..45391
                     /locus_tag="JA154_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAT7994409.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            45509..46783
                     /locus_tag="JA154_00885"
     CDS             45509..46783
                     /locus_tag="JA154_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemization operon protein LarA"
                     /protein_id="HAT7994410.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            46791..47588
                     /locus_tag="JA154_00890"
     CDS             46791..47588
                     /locus_tag="JA154_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HAT7994411.1"
                     /translation="MYFMEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYA
                     LETALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSY
                     TISQGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVV
                     EIDMPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKNEN
                     NYGLNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            47602..48822
                     /locus_tag="JA154_00895"
     CDS             47602..48822
                     /locus_tag="JA154_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAT7994412.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            48833..>50195
                     /locus_tag="JA154_00900"
     CDS             48833..>50195
                     /locus_tag="JA154_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-acid oxidase"
                     /protein_id="HAT7994413.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPK"
BASE COUNT        19782 a         5485 c         9451 g        15477 t
ORIGIN      
        1 ggaatattat taataaataa taattacaaa attgtaatct ttatttatta aataaatgag
       61 taaaataata tattaatggg tatataatta agacaatact agtttgggga ggataaaatt
      121 atggattata aaacaatcat attaaataga aagtctgtta gagaatataa gaacacagat
      181 attaaagaag aatatcttag tgaaattaag aaatactcag aatcttgtaa gaaactagtt
      241 ccagaaatag atgttgatgt aagaattatg aatagaagtg aagtttttaa taatttagat
      301 aaagtagcag ggtacaaagg aaatatgata gattctccaa gttatattat aatattatct
      361 gacataaaag ataattacat tgaaaattca gggtacatag gagaaagtat aatctttaaa
      421 gtaacagaac ttggaattgg ttcttgttgg gttacttttg aagatggaaa aaaggtaaaa
      481 gaaaaacttg gaattaattc agataaagag gtaacaggaa ttatagctat tggatatgga
      541 gaaaatgtaa gtaatgctaa agtattaaat gctacaaaaa ctgggcaaaa ttattcaaaa
      601 tctgatatgc aagttgtaac agataagggg tctactagac taggtgtaga agatgttgtt
      661 tatatgaacg aatggggaaa tgttgcaagt atagaaatct tagaagaaag agcacttctt
      721 gatgcatttc actatgcaag attagctcca tctacattaa atagacaacc atggagattt
      781 atagttgatg gaggcactgt agttttagct gttagaaaag atggtcatac taatttatat
      841 gaagaaaaaa tagacattgg tatagttatg ctttattttg caacaataat aagtgcaact
      901 atgtttgatt taaaatggaa tttaggtact ccagacaaag attataaagt gccagaagac
      961 tataagatag taggatattg taatatataa gatagtgtaa tacttaattt aactacactt
     1021 aaattaattt ttataaaaag ttcaataaca ttttacaata taatgttatt gaactttttt
     1081 attgtataat acaattttta tatttattgt attgattgaa aaatgcttat aaatttaaat
     1141 gcttacaatt ttgtgcatga atagagaggt atattatttt attaaactgt ataatttgct
     1201 tggaggtaat atcaaatgga atggataaat aaattaaatc aagctattac ttatattgaa
     1261 gaaaatctcg aaagtggagt ggattatgca gaagcagcaa aaattgcatg ttgttcaacc
     1321 tttcattttc aaagaatgtt ttcatatatt gcagaagtac cattatctga atatattaga
     1381 cgaagacgta tgactaaggc tgcatttgaa cttcagaaca gtaacatcaa aattttagaa
     1441 ctatctgaga agtatggtta cgattcacca acatctttta atagagcttt tcaaaatata
     1501 cataacattt ctccatctgc agctagagca aagggtgttg ttttaaaagc atatcctaag
     1561 atgacattaa gcatttatgt aaaaggaaat atagaaatgc aatatcgtat tgtagaaaag
     1621 aaagggtttc gtattgttgg tataaaagaa agtatgaaca tgattgtaga agagtgcttt
     1681 gagaaagttc ctaagttgtg ggctaaatgc ataaaaaatg gaacaattga taaactttca
     1741 gagttaataa ataatgaacc ttgtggattg cttggagtta gtgtgtgtac aaatagtaag
     1801 ggtttagtat tatattgctg ctccaacaga taagcctgta ttagaagata ctcatgaata
     1861 cttaatacca tcaggcacat gggcagtatt tgaatgtgtt tctccaatgc caaatgctct
     1921 ggcaatacag gaattgcaaa aaagaattat tactgagtgg ttaccaagtt ctgggtatgt
     1981 gtatgctaat ttaccagata ttgaacttta tcccaatggc gatatcaatg ctcctgatta
     2041 tactacagaa gtttggattc caattcagaa accaagttaa aattaataaa taatatggaa
     2101 ttaataaata acattaaaat taataaacaa catgaaattc taaaattaca ggctgaaaat
     2161 agtctgttat ttttttgtaa atatggaaag taagctttac ataaatggaa tatagttgta
     2221 taattatgga aagtagactt tccgtagttg agatattaaa gggggataga tgtgaaaaat
     2281 agattagaag aaataagaaa agaaagaggc ataaaacaag aagaacttgc ttctattttg
     2341 caagtatcaa gacaaactat aggttcatta gaaaatggta ggtataatcc ttctatcatc
     2401 ttagccttta agatagctag atactttgat atgagtattg aagaaatatt tatttatgag
     2461 gaggagtaaa aatgaaaaag aatatgttat atgttggaat tatatatttt atttgtggaa
     2521 ttttacttgt tttattggct acatttactg agttttcatt tgaggctttt atttggggat
     2581 tatctggagc tgctttaggt ccaggtgtat gtatgattct tcaatatatg tattggagta
     2641 aacctgaaag agcagtagat tatgaagaaa agataaaaaa tcaaaggata gagatgaatg
     2701 atgaaagaag aattatgtta cgtgataaag ctggacgtat tactaatcaa atcatgtgct
     2761 atgttcttgt aattcttata tttattgttt ctatattgag tgtattttct gtaatggtaa
     2821 taagcaagtg ggttttagtt gttcttggat tgcttatttt atttcaattt atttgtagtg
     2881 ttgttgtata caataaatta gataaaaggt tgtaataagt tatgatataa aatagaaaat
     2941 tatataaaag taaattagtt tttaaattaa tgctagaaga taaatttata taattatata
     3001 aattttaaaa agacacagta atttcaaaaa ataacttaaa atttaaatca aaaataaaaa
     3061 aactcctaat ctattgaata aatagcttta ggagttttta atggtgcccg aggccggact
     3121 cgaaccggca cggagtatta gtccggtgga ttttgagtcc actgcgtcta ccaattccac
     3181 cactcgggca tgtcatgtat attattatag caaaaaaata tattgtttgc aacatattta
     3241 attttaatat actattatta ttatgggaat attaaataac actatattgg tatataatat
     3301 atatgttaca aaaatagata atttttagga gtgatataat ttggaaaaaa aattaattat
     3361 aatagatgga aattcaataa taaatagagc tttttatgct cttccagaaa tgaataataa
     3421 agaagggtta aagaccaatg ctatatatgg gtttactaca atgctattta aaatgataga
     3481 tatatataaa ccaacacata taagtgtagc ttttgacaga aaagcaccta catttaggca
     3541 tttagaatat aaagattata aggctggaag aaagggtatg ccagatgaat tggcagaaca
     3601 attacagcca ttaaaagacc ttttagataa atttagaata aatagactag aaatagatgg
     3661 ttatgaggca gatgatatta taggtactgt ttctaaaaaa gcagaagaaa atggatataa
     3721 ggtatacata gtaactggag ataaagatgc tattcaactt gcttcagata atacaactac
     3781 ccttataacg aaaaaagggg ttggagaagt agaagaatac aactttaatt ctgtaattga
     3841 aaaatatgag atgactccaa cacaatttat cgatttaaag ggtcttatgg gagacaagtc
     3901 tgataatata cctggagtac ctggaatagg tgaaaaaaca ggtataaagc ttataaaaga
     3961 gttttctagt atagagaatc taatagagaa tacagataag cttaaaggta gtgtaaaaaa
     4021 gaaaatagag gaaaacaaag aaatagctat tcaaagtaaa agacttgcaa caattataag
     4081 agatgtgcca atagaagtgg atttagatag tatgattttt ggcgattatg ataaagatga
     4141 acttttagat aaatttagat actttggatt tacaagtctc ttatcaagat tagttgattt
     4201 ggtcgattca gatgacacag aacacgtaga agaaaaaata gaaatattaa aattaaaaga
     4261 tatagaaaaa tttatagatg aagtaaacaa aaaaggacaa gttattttaa aaactgttag
     4321 aggacaaggt aatatacttg aaaaaaatat aatatatatt tttataagtg tagatggaga
     4381 aaaaatatat tatgtagatt caaatgaagt gtcaaagtta gatagtatat tttcaaataa
     4441 tgaaattaaa aagtatggat ataatttaaa agatgattat atatctttaa aaccatataa
     4501 tataaactta gaaaatatgt attttgatat aacaatagct gagtacttaa tagattcaac
     4561 ttcatctacc tcatatgatt gtagtgcaat agctatgaag tatttatcaa aaaaggttaa
     4621 atcaaaagaa gaattattag gaaagggtgt taaatctaaa aactatgagg atttagaatt
     4681 tgaggattta gcagagtata tgggtcaaat aatatgcaca gttaaagaaa cagttcctat
     4741 gatggaaaga agtctaatgg atatgagtat ggatggtctt ttttatcatg tagagatgcc
     4801 tttagtagaa gtcttaggtc atatggagta tgaaggtata aaagtagaca aaagtatatt
     4861 ggatgaattg agtgttgaat ttaaagaaat aatagctaca ttagaaaagg aaatttatga
     4921 attatcagga gagccattca atataaattc accaaaacaa ttgggtgtta tattatttga
     4981 aaaattagag cttccaataa ttaaaaaaac taaaactgga tattcaacta atgcagatgt
     5041 tttagaaaaa ttaagagata agcatccaat aatagataag ataactgagt atagacaaat
     5101 agtaaagtta aattctactt acgttgaagg acttttaaat ataataaatc cagaaagtaa
     5161 tagaatacat tcatcattta atcaaactat aacgactaca ggaagaattt catctacaga
     5221 accaaatctt caaaatatac ctataaaaat ggaaatgggt agaaaaataa gaaaagtgtt
     5281 tattgcagat gataactgta agttggttga tgccgattac tcacaagtag agctaagagt
     5341 tcttgcacat atgagtcagg atgaaaatat gatagaagca tttaaacatc atgaagatat
     5401 tcataccaaa acagcttcac aagtttttaa tgttcctatg gaagatgtta ctagtgaatt
     5461 gagaggtgca gctaaagctg taaactttgg tattatatat ggtaaaagtg actttggtct
     5521 agctgatgat ttaaatatac ctgttccaaa ggcgaaagaa tatatcgaaa gttattttgc
     5581 taaataccat aaaataaaag agtttatgga cacaacagta gaaaaggcta gtgaggatgg
     5641 atatgctgta acaattttaa acagaagaag atatatacca gaaataaagt caagcaattt
     5701 tatggaaaga aatagaggaa agagatttgc aatgaatgca ccaatacaag gaagtgcagc
     5761 agatataata aaaattgcta tgataaatgt tcataaaaaa ttggaagaga acaatttgaa
     5821 atcaaaactt atactacaag ttcacgacga gttgatagtt gaagcaattg atgatgaaat
     5881 agatatagtt aagaaaatag taaaagaaga aatggaaaat gctgtaaata tggatgttca
     5941 cttagatgta gatttaaatg taggtagttc ttggtatgag acaaagtagg tgattttatg
     6001 ttgatattag gacttacagg aggaataggc tgtggaaaaa gcagcctatc aaatatattt
     6061 agaaatctta acattcctat agtggatgct gatattatat ccaggaagat atttgaggat
     6121 aagttgttat tggaaaaagt atttgtccat tttgggcaaa gtataaaaaa tgatgatgga
     6181 actctaaata gaaaagctct tgggaaaata gtttttagtg atgaagaaaa acttaaggaa
     6241 ttaaataact tgactcatcc acgaataaga gaaaaaataa taagtgaaat agaaaaattg
     6301 agaaaaaaag gcgaaaatat agtagtgtta gatgcggcaa tattggtaga aagtggtttt
     6361 ttagatatgg ttgacaagct attagtagtt acttgtaaac aagaagtaca aattagtaga
     6421 atacaaaaga gagataattg cagtgagcag gaagcactta gtaggataaa ttcacagatg
     6481 agtcaggaag aaaaatccaa atatggtgat tatataatag ataattcggg aacaataact
     6541 gaattagaaa gtaaagcaca taaatttatt gagtacatga aggagaattg gcgtgaatag
     6601 taagaaagta ttgattctat ctatttttat aatcttattt ggggcactat taatggaaag
     6661 caaagtaata cataaatttt tatatcctaa aaaatattca gagtatgtag aaaagtattc
     6721 gaaagaattt aatttagatg aaaatatagt ttacagtgtt attaaagccg aaagtaagtt
     6781 taatagttct gctgtttcaa aaaaggaagc aaaaggatta atgcaaatat tagacataac
     6841 tagagattgg ggagcagagg aactaaattt aaaaaatgtg gatattttcg acccagagac
     6901 taatataaga cttggctgtt ggtatttaag taagttatac aaagaatttg gtaaattaga
     6961 tttagtgata gctgcatata atggtggttc aggtaatgtg aaaaaatggt tagaaaataa
     7021 tgaatatagt aaagatggcg aaaatctaca tgatatacct tttaagcaaa cttcaaaata
     7081 tgtagaaaaa gtaaaaaata attacgaaca ttataataag atatatggca agaaaggaaa
     7141 aaactaatga agagaataaa agtcttaaca gtcgttttag ctataactct tatggtagct
     7201 ggttgtagta atactaaaac aaaacaaagt agtagtgatt caagtttaca aagtcaaagt
     7261 tctatagatg ctaagtatat aaacttaaca atggtaactc caaaaactat aaatcctata
     7321 actaatactg ataagtctgt tggttatata atgaatttag tttatgatag tttatttact
     7381 atagatgaga actacaatgt aattcctcaa ttagttaagg agtataatat agctcaggat
     7441 ggaatgagta tagatataaa gctaaaagat gcaaagtggc atgatggaaa aaatattact
     7501 tcaaatgatg taaactatac tattggttta attcaaaaaa gtgcagatag tccatataat
     7561 gaatttacta aaaatatagc ttctgtgaat attaaatcag ataaagattt tacaataaaa
     7621 tttaaagcta gatatgcttt ttcaattgat agtttaattt ttccaattgt atctcaaaat
     7681 catttagatt caaaagatgt aaatgacaag aataataata tgataggaaa tgggaaatat
     7741 aaaatagaat catatacaga aagagaagga atggttttat ctgtaaacaa agattactat
     7801 gaagaagttc caaaaaccat gaagaacata aaagtaggta tggtgcctaa tgaagatgca
     7861 aggacatcta tggttatggc tcttgatagt gatataacca atgttacatt aaatgattta
     7921 agtaagtttc aagaaaaaga gtttaatata acaaaatatc aaggaagaga ttatgagtgt
     7981 gtattattta attataataa tccttttttc aaagatgtta attttagaaa agcaattgca
     8041 cactctatag ataaagatag aattattagt gaaggttata tggatgatgc tactccagtt
     8101 aattttccac ttaattcaaa atcaaaatac tataatagtg aaatgaagga tttagagttt
     8161 aataaagata aggcgataga gtgtcttgct aaggtagaat atgcaaatgt aaactcagta
     8221 aatcaaaatg acaataaagt aaaaaatcag aaaaaatcag ctaaaaagaa ctcaaagaag
     8281 cttacgccag aagaagaagc aaaagctaaa aaagcagaag aagatagaat caaaaaagaa
     8341 gctgaagaga aaaaaaatag agaaaaagag gaagtaaaga aaagtctttc tgaaatgaac
     8401 ttaaagataa tagtaaacag agataatagt gagagaataa aaactgcaaa cataataaat
     8461 gaaaacttaa aagctatagg aataaatagt acagtaaatc agctgtcaga taaggatatg
     8521 gaaaatgctc ttaattctaa aaattatgat ttagcattag ttggatggaa actatcaatt
     8581 ataccagatg caagtagtat tatagcgagt agtggatata ctgatgataa gttaaatgga
     8641 tatatgtctg cacttacaag ttctacgtct gaaattgaaa caaaaaaagc atacaaagat
     8701 gtacagacat atataaaaga taatgcaaca tttataagtt tagctgtgag aaacaactat
     8761 attgtaagta atagtagatt aaaaggtaaa attactccaa atgattttga tgtatatgag
     8821 ggaatatcta atttagatat aaaatcgaat gaatcaaatt aaatgattaa tttctattta
     8881 aaaagtataa actatatgat agaataccct tatagggtat aaggaggtaa ttaaatgaag
     8941 aagaaactat tagtagaagg tatgagttgt ggtcattgtg taaaccatct taaaactgca
     9001 ttaacagaag atatagatgg tattgaagtt ttagatgtag atttagaaaa taagtgtgca
     9061 tctgtagata tgaaagatga tgtatctatt gaaaaattaa aagaagtgat agcagaatta
     9121 ggatttgaac taaagggaat tgaatagaaa ttgataaact aaaaaaagac ctctattggg
     9181 ggagtaatag aggtctaaaa atgggggaga ttgagtatgt ttgattttac attactatta
     9241 tctccccttt taaattaata tatacataaa aagtaaaaaa attttgtaat gactcacatt
     9301 tcttgagctt tttgaatata ctataaatag ctaagggagg gagtatcatg gaatctaggg
     9361 gatataaaga ggatttaaaa aaaatgatac ttgatatggc ccacaaagaa cttgagagtt
     9421 atgtatcaca aaaaggagca aaaaaatact ttgaaggtaa cttggataag attattaata
     9481 ataatataaa aaaattcaat caaagtattg gtcttaatag agtatattta aaaatgttaa
     9541 aaaagtgtgc atatcaagaa agtgtaactt caatatcaag aactataaag aaagaagcat
     9601 tatttaaatc aaaaaaagag ctatttgctt tttcagagta cttaaaactt gatattaaca
     9661 agcgactttc atataatcaa attttaagaa aaatatcaag atatatatac tacaatagaa
     9721 gttcatatgc tcaaaaatac gttgttttca aaagaggaga tgaagaatat cttttagaac
     9781 cagagaaaat aaaagatgaa ttaatatcaa gctataggtc aaagactaga gaagatatga
     9841 ggtcaatagc aaaacttttg gatatagaag tagaagatga ttataatgct gaagatatta
     9901 gaaaaaaagt aataaattat attattaaag aaaaacttaa aagttagagt tttaaagaat
     9961 aagttctgcc aatttgcatg attaagtata acttgggtat aaatttttta tgcatttgaa
    10021 agagcttatt ctttttttga catattcttt taaataattt accatgattt ggtaattggt
    10081 catcatcatt caaattttta acttaatatg atttaaataa atttataata atgatataat
    10141 ataaaaaaaa taaaaattgg agagtgattt caaaatgaag tttaattttt gtcataacaa
    10201 tttcaatgtt actaatttag aaaaaagttt agatttttat aaaaaagctt tagggcttaa
    10261 agaggtaaag cgcaaggaag cagaagatgg aagttttata ttagtttatt taggagatgg
    10321 cattactagt catacattag aattaacttg gcttagagat tgggatagac catataattt
    10381 aggagacaat gaatttcatc tggctttaga agttgaggag tttgatgagg caaaaaaact
    10441 tcataaagat ttagattgca tatgttttga aaatgaaagt atgggaattt attttatagc
    10501 ggacccagat aattattgga ttgaaatact tcctaaaaat cactaatttt aaggatatgt
    10561 ttataatttc tttacaatta ataagaaaat aagttatcat taaattgtag ggactaaaaa
    10621 tggataataa tgttatgaat ttatgactat aaaaatttgt tggggggagt gatagtagtg
    10681 aaaaaagcta tagcagcttt aggaattggg gcagtagcag tatctgttag ctccataaat
    10741 gcaagtgctc ttgaaaaggg aacagtaaca gcaagtgcct tgaatataag aagtggacca
    10801 agttctgatt gcgataaggt agcaaaatta tacaagggaa aaactgtaga gattttagaa
    10861 aagtctaatg gatggtataa agtaagggta tcgagttctg ttgttggatg gggaagtgca
    10921 aaatacatat caacaagtgg ttcgtctgaa ggtacatcaa gtcaaaataa tccaacttca
    10981 agtgggacaa ctattagtgg aaatggtaaa gtaaatgtga gttctagact gaatgtaaga
    11041 agtggtgcag gtactaacta ttcattagtt ggtaaagcaa ataatggaga tgtagtaaag
    11101 ttactagaac aaagcaatgg atggtataaa ataaaactat caaatggagt tactggatgg
    11161 gctagtagcc aatatatttc taaaacatca gaagatgttg gaacgaacaa ttctagtaat
    11221 tcaaatagta ctaataattc tgataaaaaa ccatctagcg aagaatcaat tgaaggcaag
    11281 aatggtaagg taacatctgc tgtgagttta aatgtaagaa gtggtcctgg gacaagctat
    11341 tctataatag gaaaattaaa tggtggagat gttgttgaat taaaagctaa aagtaatggt
    11401 tggtataagg taaagttatc gagtggaaca attggatggg tgagtgccag ttatatttct
    11461 gagactaatg aggatacaaa agaaaaacca aattcatcat ctaaccaaaa ttctcaatct
    11521 aatagtaact caaagccatc atttactgga aattcagata aatctacagc aaaaggttca
    11581 acaatcgtag attttgcata tactcttatt ggaataccat atcaatgggg agcttcagga
    11641 cctgataaat ttgactgttc tggatttaca cagtatgttt ttaaacattc tgtaggcgtt
    11701 tctattccta gagtttctag agaacaggct aattttggaa gtgcaatttc tatgggaaat
    11761 tatgcaccag gagatttagt ttattttgac actgatggtg atggaacaac taatcatgta
    11821 ggtatatacg taggaaatag taaatttatc cattgtagtg gtactcagac taatcctaat
    11881 aaggttaaag tggataattt aactagttca tattggtcta aagtattact tggtgcaaga
    11941 agatttgttt agtaagtaca atttcttatt aaaaaattag aagaaataat tatttatatt
    12001 cataaattgt gactcttaaa aatatacttt attaataata taattttggg agggacaaac
    12061 taatgaatag aagaaaaacg atatacagag gatttaataa taatagacat aagattgatt
    12121 tacgtaaata tgttataacg attgcttgct tgtgtctaat tggctattat tcttatacaa
    12181 aaataaaaga tagcaagata ttggagtatg tatcggctaa aataccattt ctaaataatt
    12241 catcagatat aacttataaa gatatatctg atgaattaaa ttcaataaaa aaagggaaaa
    12301 aatctaaatc acaaaccaat tcagatgata aacaagaaac taatccagaa aaagcggtaa
    12361 ataacactaa agagccagaa gaagtcaaat tagctactat agaaggctgg gatatgtata
    12421 ctattcaagt tgctgcaata gataataatg atgatttgaa aaaaatacag acatcattag
    12481 ttaataatga catacctttt tcagttatgg agaaggatgg agtaaaaaaa atacagacct
    12541 attcctcttt tgatgaaaat gatgttagaa agcagataag cagtgtaaga aaggtgtttc
    12601 cagatgcatt tttatcacac ttagatgcac ctatgttgtc tttagagtat acaagtaact
    12661 acgcttatat agagagtata tctaaagaat taaataaatt aataacaaac tttaaagaag
    12721 aatcaagttt ttggtcgaat gctgaaaata atgtagatat ggaaaaatat aatactattt
    12781 tgacaaatag aaaggcaata tcacaaaata tatcaaaaga agctgaaaag attgattatt
    12841 ctgaaatgcg tttatttaaa gataatttaa tagagtatgt aaagaatgtg aatgaaaaaa
    12901 ttgatacagc ttcaaaagca gcaaatgaag aaaaatatag tgtaagtaaa agtctgcttt
    12961 tatcatccat gcaggagtac tctatgttta taaattctat aaaataaaat acagaaaatt
    13021 tggactttag aaagctatat gaaaaggatt gtttaattaa gcaatccttt tcctcaattt
    13081 gacttaatat agaggtgttg atttataaaa taaattataa aaatacggat atttttttaa
    13141 caattgttaa gattttttaa tatatatagt atacttatag aaggcagaaa aaatgttttt
    13201 ttatagataa gggggtaaaa caatgaaact cttaactttt aagggaggga tacatcctcc
    13261 atatagaaaa gagtactcta acacaaaggc acttgaaaaa gctcaagctc caaagatagt
    13321 ttacattcct cttcagcaac atataggggc accagctaaa cctattgttg aagttggtga
    13381 tgaagtaaag tttgggcaaa aaataggtga acaacaaggt tttgtttcat gtaacgttca
    13441 ttcttcagta tcaggtaaag ttattgctat tgaacaacat gaagtaccag gaggttcagc
    13501 tcaatgtgta gttatcgaaa atgactttaa ggaagaatta catgagagtg ttcaaccaaa
    13561 aggtcagtta gaggatttga gcaaagaaga tatagtaggt atcataaaag aggcaggaat
    13621 agtaggtatg ggtggagcta cattccctaa ccatgtaaaa gtatctcctc caccagatag
    13681 caaggcagaa gtagttatat taaatggtgc agaatgtgaa ccatacttaa ctgcagacca
    13741 cagattaatg gttgaaaatc cagaagatgt tgtgtttggt ttaagagcat taatgaaggt
    13801 attagatgtc aaaaaaggat ttataggaat tgaaacgaat aaaccagatg ctatagaagc
    13861 aattcaaaat gtagctaaag actatagcga aatcgaagtt gttggccttc aagtgaaata
    13921 tccacaagga gcagagaaac aacttattta tgcgtgtaca ggcaaagaag ttccatctgg
    13981 tggattacca atagcagcag gtgcagttgt tgacaacgtt gccacagcag cacagatagc
    14041 taagtctatt aaaacaggta tgcctttggt tgaaagaatc actacaataa caggtagttg
    14101 catcaaagag ccaaagaacc taataacaaa agttggaaca ttagtttcag aaataataga
    14161 acaatgtggt ggatttaaag aagataagaa gattggtaag gttataatgg gtggacctat
    14221 gatgggaata gctcaatata ctactgaaat agcgactaac aaaggttctt caggaatttt
    14281 atgtttggat gaggaagaat cacgtactcc agatattcaa aactgtttaa gatgtggaag
    14341 atgtacagat gtatgtccat cattcttaca accacttttc ataagtgcat attctttaaa
    14401 agatgactat gacacagctg aatatcatag agcaatggat tgtatagaat gtgggtcttg
    14461 ctcatttatt tgtccagcaa gaagaccatt acttcaatct ataagatcag caaagagaga
    14521 aataggagca aagagaagaa agcaagctgc tcagaaataa agaaagttaa gggggaaaaa
    14581 cgatggaaaa taagttgata gtatcatctt ctcctcatgt gagaagtaat gaagatactt
    14641 catatataat gaaacaagtt attatagcac tccttccagc agcagtagca ggagtatact
    14701 tctttagact taatgctttg agtgctatgt ttttttgtat acttggtaca gtaggtactg
    14761 aatttttata tcaaaaattt acgaagcaaa aaagtactat aggagatttt tcagctgttg
    14821 taacaggatt attattagca tttaacgtac cagcatcact tccttggtgg atgtgtctag
    14881 taggaggaat atttgcaata ttagtagtta aaatggtatt tggtggaatt ggatgtaact
    14941 ttgtcaatcc agcacttgct gcaagagcat ttttattagc atcattccca gtagcaatga
    15001 ctgcttggac tcaaccaggt gttaactgga taggtaaaaa tttagatgca gttactacag
    15061 caactccatt aagctttttg aaaaatggag cagcaggact ggcagatatt tctagcaatg
    15121 gaattagtct tgctgacatg atgataggga atattggtgg atgtataggt gagacatcag
    15181 caatattatt attattaggt ggagtatacc taatgtataa aggcataata aattatgtta
    15241 taccagtatt ttacatagca actgtattta tattaacatt ccttttaggt ggatttaata
    15301 taactttcgc aatatatcaa ctgtttgctg gaggacttat gttaggtgga ttcttcatgc
    15361 ttacggatta tacaacttcg cctatgacta aaaaaggtca aataatatac gctgtattag
    15421 ctggtcttat aacaactgtt ataagaatgt atggtggata tccagaaggt gtatcttact
    15481 caatactatt agtgaactgt cttgctccac ttatagataa gtttgttaga aatagagtat
    15541 ttggggaggt ggctaaataa tgaacagtat ggtgagatta ggtggtactt tacttgctat
    15601 aagtgcaata gctgcattag ctcttggagc tactaatcag gttacagctc cagtaataga
    15661 acaaagaaat atacaagcaa ataatgaact tagaaaagca gtccttccag aagcaaaaga
    15721 atttaaagaa atgaaggaag atacttacaa aggccttgga gatggtctta tagctgaagt
    15781 gtatgaaggt ttagatggtt ctgaagtggt tggatatact ttaaaagcta aaccaagtgg
    15841 atatggtgga gaaattgaag ttatggtagg tatatcatct gaaggtcaag ttacaggtgt
    15901 tgacattggt aatatgtcag aaacagcagg tcttggagca aaagctaagg atgatgcatt
    15961 taagggtcaa tacaaaggaa aaactgctga gcctcttgaa gtagcaaaag gaagtacaac
    16021 agcagataat caaatattag caatatcagg tgcaactatt acatctacag cagttactac
    16081 aggagtaaat gctgctatag atgttttcaa tagtgctctt aataaataag gagggttaat
    16141 tatgaattta gctaaagtat ttaaaaatgg gctaatagat gagaacccaa catttgttca
    16201 agttataggt atgtgtccaa cactagccgt tacaacttca gcaataaatg gaattggtat
    16261 gggtctttca acagcagcag ttttaatatg tgcaaattta gttatatctt taataagaaa
    16321 aattacacca gataagataa gaatacctat atttatagta gttatagcta catttgttac
    16381 aatagtaggt atgcttttaa aagcatatgt tcctgctctt gataaggctc ttggtatata
    16441 tataccatta atagttgtta actgtttaat attagctcgt gcagaaagtt ttgctttcaa
    16501 aacaggagct atgccttcaa tagttgatgg tgtaggtcaa ggtcttggat ttactgttgc
    16561 acttacaata ataggtgcag taagagaatt acttggaaat ggtagtttat ttggaatgac
    16621 attatttgga gcatctttcc aaccagtatt aatatttata ttaccaccag gtgcattctt
    16681 aacattagga ttcttatttg caggatttaa taaattaaga agtaaaaaag catagtggag
    16741 gtgtaataaa tgaatttaat acttttattt ttaagtatcg ttcttgttaa taacgttata
    16801 acatctcaat tcttgggtat atgtcctttc ttgggagttt ctaagaaggt tgatacagca
    16861 gtagggatgg gagttgcagt tacgttcgtt ttaactctag cttcaattat aacttatttt
    16921 atacagattt tattagtaaa aactggaact ggattcttac aaacaatagc ttttatattg
    16981 gttatagctt ctatagttca gttcgttgaa atggtaattc aaaagatgag tccatcttta
    17041 tatcaagcat taggagttta tttacctctt ataactacaa actgtgcagt tcttggtata
    17101 gctctagtta atgttcaaaa aagttataat ttagtagaaa caataataaa cggatttgga
    17161 gccggagctg gatttacttt agcaatagta atattcgcag gtataagaga aagactagaa
    17221 ttagctgata taccagaagc atttaaaggt ttccctataa cgcttatatc agcaggttta
    17281 atgtcaatag ctttcttagg atttacagga cttataaagc tataaaaaag aggggtgaaa
    17341 atggtgatac ttacagctgt attagtttta ggaattatgg gcttaatctt cgggatagta
    17401 ctagattttg catctaaaaa gtttgctgta gaagtagacg agagagtgga agctatacta
    17461 ggagtacttc caggagccaa ctgtggaggt tgtggatttc caggttgtgg aggtcttgca
    17521 aatgcgatag ttgaaggcaa tgcaccagtt aatggttgcc ctgtaggtgg agcagatgtt
    17581 ggtgcaaaag ttggagaaat aatgggaata agtgctgaag caggtgagaa acaagtagca
    17641 aaagttattt gtaaaggaac ttgttcaagt gcaaaagata aatatgagta tgaaggaata
    17701 tcagattgta gagcagcaaa tgttttaaac tcaggtgcta agatgtgtaa atttggatgt
    17761 ttaggtcttg gaacatgtaa agatgcttgt aaatttgatg ctatttctat agttgatgga
    17821 atagcagtaa tagatgaaga aaagtgtgta aattgtggaa aatgtaaaga agtttgtcct
    17881 aagggtataa taatcacaaa accagaaagt caagaagtag ttgtagagtg taatagcaaa
    17941 gagtttggta aagctgttaa agaaaaatgt actgctggat gtattggctg tggaatgtgt
    18001 gtaaaagctt gtaaatttga tgcaataata tttgaagaca aaatagctaa gatagaccct
    18061 aataagtgtg taggttgtat gcaatgtgta gcaaaatgcc ctacaaaagt aatatcaggt
    18121 gacataacta agaaaaagaa agttactatt gaccaagaac tttgtgttgg atgtacagtg
    18181 tgcaaaaaac aatgtaagtt tgatgcaata gagggagaat taaaagaaaa acataaggta
    18241 gatgcagata aatgtgtagg atgccattta tgtatggaaa aatgtccaaa gaaagctatt
    18301 aaaattttgt aatatggaca aaataaggat atggaataat ttccatatct ctaatgagta
    18361 cataaaacag aggtatggaa taatttccat acctctattt tatgtatatt tattctaatg
    18421 gtaataaata taacaatagt gtaacaaaaa aggttcgaca tttctgttga attttttaca
    18481 atatagtgat agactaaata ggtacaaatt acgtacaaat gaggtgaata aatgaatata
    18541 atcttggcat cagcctctcc aagaaggaaa gaaattctag agaatacgaa tgtaagattt
    18601 gatataatta aaaatgaaat tgatgagatt atattggaag gtgaaacacc aaaacattta
    18661 gttatgagat tagcttttga aaaaagtatg tctgtagcat ctgaacataa tgaagatgtt
    18721 gttattggag ctgatacagt tgttgtttta gacaatgcta ttttaggaaa accaaaagat
    18781 gagtcttgtg caagagatat gttaaaaaga ctatctggaa gagagcatca agtcataaca
    18841 ggtattagct taataaactt gtgcgaagat aaaaaggtaa ttgactatgt aatcagcaat
    18901 gtgaaattta aaacgttatc agagcaggat attgaggatt atctgaaaac taatgagtca
    18961 tttgacaaag caggagccta tggtatacag ggttatggag ctttgttagt tgaagaaatt
    19021 agaggagatt attttaatat agttggtctt ccaatttcaa ggttaggcga cctattaaaa
    19081 aaatatttta gtataaatct tttttatgga gtgtgattta tctgaaaaag tcatttaatt
    19141 caaccattaa ggtaaaagaa atggcaccag aggaaagacc cagagaaaag atgcttgcca
    19201 aaggcgtaaa aagcttatca aatgctgagt tgttggctat acttttaaga actggcaaca
    19261 agaataaaaa tgctattgaa ttagccaact atataataaa tagagatatt cagggtatta
    19321 gacatttaga agatatgaca atagaggagc tgtgtaatat agatggaatt gggttatcaa
    19381 aatcaaccca aattaaagca gctttagaac taggctctag agtagctagt tttaaaccta
    19441 taaagtataa aataaggaat ccttgggata tacagaggta ttatatggat agcctaaggt
    19501 atttaaaaaa agaggttttt aaagcggttc ttttaaacac aaaaaatgag ataatatctg
    19561 atgtagatgt atctataggg actttaagct cgtcattagt ccatccaaga gaggttttta
    19621 aagaagctat aagaagaagt gcaagtaaga taatagtgat gcataatcac ccttcaggaa
    19681 gtgtagaacc atcaagagaa gataaaaata tcacatcaag acttattaaa tgtggagaaa
    19741 taattggaat agaaataata gaccatataa ttattggaga tggattatac tttagcttta
    19801 aagaaaatat gataatttga ctttatatgt gataaaataa taggagaatg ttttaaacat
    19861 gattcttaat agtaggaagg agtaaaatca tggctaagga aaaaaagaaa gaaaaaaaag
    19921 gtttttttag ttttaataag atgacaaagg atatgggaat agatttaggg actgcaaata
    19981 cattagttta cataaaagga caaggtattg ttgttagaga accatcagtt gtcgcaataa
    20041 gagatgatag taaagaggta ttagcagtag gtgaagaagc caaaaaaatg atagggagaa
    20101 caccaggaaa tatagtagct ataagaccta tgaaagatgg agtaatagct gattttgaca
    20161 taactcaatc aatgattagc tattttatac aaaaagcggc tgataaaaaa ggtgttgtaa
    20221 gtccaagaat agctatatgt gttccttttg gagttacaga agtagaaaag agagctatag
    20281 aagaggctgc aagacaagca ggagctaaag atgcattcct tatagaagag ccaatggcag
    20341 cggctatagg ggctggatta aaagttgaag aaccagaagg taatatggta gttgatattg
    20401 gtggaggtac ttcagaaata gctgtaatat ctttaggagg aatcgttact gctaaatcta
    20461 taagaatagg tggagacgaa tttgacgagt ctatagtggc ttatgtaaaa aaagaatata
    20521 atcttatgat aggtgaaaga actgctgaaa atgtaaaaat taacataggt tctacattta
    20581 aagatgatga agaaataaat atgcaaataa gaggtagaga tttaatctca ggattaccta
    20641 aaacaataga aatatgctct acagaagtta gagaagcatt aaaagaacct gtaagttcaa
    20701 tagtagatgc aataaaatct acactagaaa gaactccacc agaattagcc tcagatataa
    20761 tggaaaacgg aataatgcta acaggaggag gcgctttgct tagaggtctt gataagctta
    20821 ttacacagga aactggtatg tctgtacaaa tagcagaaac acctcttgat tgtgttgcac
    20881 ttggaacagg taaatctgtt gaagaccaag aaatctttga aaaagtatta atgatgaata
    20941 ctaagaatta gaagttggtg atggccttga gatttgacaa gaacaaaaat gagaaaaaaa
    21001 ggattaatgt taaagtgata gcaacgggcg tagttgctat cactttaatt ggaattgtgg
    21061 gtatatcaat tggtaaattt tctagtggta gtccagtaaa tttaggagta gcttcagatg
    21121 ctattacaag tgtaggtaag ggaataaatg atggattttc ttttatcaaa aatggtttta
    21181 aagatgtagc aaattttaaa gataattcta aaaaggtaaa aaaattagaa gaagaaaatg
    21241 aaaaacttaa aaaaaatatg atagctttaa atgccaaact agataaaaca gaatcgctag
    21301 aagagttaaa aaagacactg aactttgtac aagaagagta taaggcgaca agtatttcag
    21361 caagtgttgt tggtaaaaat gatggaaact ggtatgagag ttttgtcata ggtgcaggaa
    21421 aaaattcagg tgtaaaaaaa gaaagtatag ttatgaatgg tagtggatta gttggcatag
    21481 tttatgaggt ttccaacaat tatagtaagg caatttcact attggattct aaggcatctg
    21541 taagctttaa actagcaaag gatgccaatg ctaaaggtac aattactcaa aatactactt
    21601 tagataataa agatagttat aatagtaaag gatatttgca aggatatatg tttgactcat
    21661 catataatgt aattcaagga gatataataa caacatcagg tcttggattc ttcccagatg
    21721 gtataccaat tggcgaagtt gaaaaagtgg ttgatgataa agataagtca ttaaaatatg
    21781 tagttgtaaa accttatgtt gattttaaga atataaatga cgttgtagtc atagaaccaa
    21841 gaaatatagg ataaagggga taaaaacaat gaaaaaagtt ttactttgtc tgttggggat
    21901 tgtgcttatg actatagaaa atagtataac aaattatata gatatttttg gcataagctt
    21961 taatttagtt cttatatatg taaccataat ctctctttat ttagatgaat tagaaggtgg
    22021 gattatagca gcaataatag ggcttattaa agacataacc gttggtggaa tttttggtgt
    22081 aaatgcatta attttgttta ttattgcata tgcaattgga tacatgagag atcagctata
    22141 taaaggtagt tatattatta ctttcgtttt aatttttata ggcagtttaa ttgattcaat
    22201 tgtcaatata ggtacatcta gtataattta tcaaagctac aacatatcaa cactttttgt
    22261 aaaaggtcta ctagtagcac caatagtgaa tagtttaatt ggtttagtaa tatatcgctt
    22321 atctaaaaga gcagttttaa aacttaaaga agattagttg gtgatttcat ggaagaaaat
    22381 aaacaagttg atagactaaa aataattaag atagtcatgg gcgtgatatt tttagctatt
    22441 ttagtaaaaa taatctatat gactacattt aaatatgaat attacaatga gttagctgag
    22501 aataaaacat ataaaaagtt ggcaatagaa gctccaagag gagaaataaa agatagatat
    22561 ggaagactgt tggcaggaaa taaaaactta tttactgttc aagtttcagg taacgatatt
    22621 aataagaaag atgcaaataa gcattcaaga gcaaatgaaa tatcattaaa acttataaat
    22681 ttgctggaga gaaatggtga agaatatgta gatgagtttc ctatatatgt agaaaatgga
    22741 aagtactatt atacatatga cagagatatt agggaatata aatcagaaaa tggtattcca
    22801 aatgattata atgctaagga aagtttttat tacttggtgg acaaacttat atcagctgga
    22861 atattatctc aagaagataa aagattggat gcgacaagat tacaggcaaa actaaatgaa
    22921 aatggatatt atccacctat tttagttagt aaatggatgt tcacagcaga aagagataag
    22981 agagattggt tggctagtta taagataaaa gaaactaaac tgagtgccaa agaagcgttt
    23041 gaaaaagtaa gaaatagtga tgcattagaa attgataaaa gtttgagtga tgaagatgca
    23101 agaaaaataa tggtcgtaag ggatttgata aaatcaaaag gatattctca atataatcca
    23161 gtaactattg ctaaggatgt gggagaaact acgattgctc aaattgaaga aagtgctatg
    23221 gaccttgttg gtgtttcaat agcagtagaa ccagtaagat attatcctaa tgggtcatta
    23281 gcttctcata tgttaggata tgtaggaaaa atgccatcca ctcaaataga atcatacctt
    23341 caaaaaggtt atgaaactgg agatatggta ggtcttgcag gagtagaaaa gagtaatgaa
    23401 agtagattaa gaggaactga tggttataaa atggttaaag ttgatgcatt gggtagaatc
    23461 agtaaagaaa ttgaatctaa aaaacctaaa tcaggagata ctgtgtatct tacactagat
    23521 aaagatttac aagaagtttc agataatgca ttaaaacaga taatcgaagt tgcttctaaa
    23581 ggaggtactt ttaaaagtaa atttggggac aaaccaatta gtgcatatgc aggaaaagca
    23641 cagtcagcag ctcttatagc tattgatgtt aaaaatggag aagtattagc ttcatcaagt
    23701 tatcctaatt atgacccaaa taaatttgca aaaggtattt ctacagagga ttataaagca
    23761 cttcaaccaa aaaatccaaa tgacttacta gcaggaagtc cacttttaaa cttggttaca
    23821 caaggggaat ttcagccagg ttcttcattt aagatgctaa cttcaatggc agcgttagaa
    23881 aatggtctgg atccaaactt tactataaat gaccctgggg ttataatgtt aggtaaaaaa
    23941 tcttttggtg actatgtatg gaaccatggt agaggaaacc atggtatgac aaacttatat
    24001 aaagcaatac aagagtcttg taatatatat atggcaacaa ttggtacagg taaaacatgg
    24061 ccagatggaa aaagcattgg tatagatatg aatgcaaata aaatacttga gtatgcaaaa
    24121 ttatttgggc ttgaccaaaa tacagggctt caagatgaag tagaagaaag agcaggtaaa
    24181 gttcctagca cagaagataa attgaaatca acacaagctc ttctaaagtc aaatttagaa
    24241 agagaaatgg caaatgattt tgttgatata actagagaaa aaaatcctaa agaatatgag
    24301 aaaagaatca atgagattgt aagctgggca gcagaaaaga aaactcctgg tagagttgaa
    24361 acaatgaata ggctaaaaaa attggatgtt aaagaagata gaattgaaga agtagcagat
    24421 ttagcagtat ttagttattt taattttgca aaatggagta ctgcagatac attcaactta
    24481 gctataggac aaggtgaaaa tgcttataca ccagctcaaa tatcaagata tgtagctgct
    24541 atagcaaatg gaggtaattt agtagaatta tctgtagttg atagagctgt ttcaagtgat
    24601 tattcatcag ttaaaattaa tgaccagaag aaagtcgaaa aaataccttt taaaaatcca
    24661 gataacttaa aagaattaac aaaaggtatg aaattggttg caagacaagg tacagcgaag
    24721 agtgcttttg ctgattttcc aatagatgta gcagcaaaaa ctggtacagc tgagaaaagt
    24781 gggaaaatac ctacagataa cgagtatgaa tatttaaaaa gtcatatgtc atcttataat
    24841 gtcaacctaa atgatgcgat taaactagct gataagatga aagcagaaaa agaaaaagag
    24901 ctttcattag ctaaggaaaa ggaaataaaa aagaaattag aaaataaaga tttgaaagac
    24961 gaagaaagaa agaaattaga ggaagaatta gaagatggag taaaagttag attagaggac
    25021 actgataagg tgaattcttc atatctaaga aaagctataa aagaattaaa tccaaaaata
    25081 acagatgacc aaatagatag atttaaacaa gattatggtt cttttacgtg gactgttgct
    25141 tttgctccag cagatgaccc agaaatagct gttgtatgtg taattcctca aggtgattca
    25201 agtgtattct cacttttacc aactagagaa gttattggaa cttatatggg attagagcca
    25261 actaatagta aaaatgacaa caagacgaat gatgttaaca attcttctga cgaaaatata
    25321 aatttcgagt cacaaattaa tagatagctc aatctatgta taaactttag ctttatatag
    25381 aaaaaataaa agggtttaaa gcgtatttga agaatataaa tgatatatca ctctggaggt
    25441 tgttatgtct ttaagagaaa tttgtagtca agaactagta gagttcaagg gtaataaaag
    25501 agggataata gtaaatataa aaagagaagc tccatttgag gaaatacagg aaaaaataat
    25561 aaacaaattg gaagcatatg ttggcttttt taatggggct aaaattagta aaattaatag
    25621 tgattgttta actgacatgg agattctaga acttaaagaa ggaattactt ctagatttga
    25681 tgtggaattt gttgaagatc aaaaaattga agaaaatagt aattttccaa caaaatatgt
    25741 caatacccta agatctggtg aaaatattga atttgaaggt gatgtagtta tattgaatga
    25801 tatgaaacct ggttcaaaag tcctttcaaa atcaaataca gttgttatgg gagatataaa
    25861 tgctggtgct aaggttgtag caggaggtaa tgtttttgta atgggtaaaa tagaaggttt
    25921 tgtacatgca ggtgcagaag gcaatgaatt tgcatatgtt gttgctggaa atcttaatcc
    25981 taaaatatta caaatagcag ataatattgc agaagctcca gatgatgaag aaaattatga
    26041 aagtgaatca gaaattagcc cagaaatagc ctttgtaagt aatggaagaa ttgtaatcga
    26101 aagctatttg tcaaagttag acaaatagaa agaaaaatag ggggtagtat tatgagcgaa
    26161 gttatagtta taacatctgg taaaggtggg gttggaaaaa ctactaccgc agcaaactta
    26221 ggaactgcgc taagcctaga aaataaaaaa acagtagttg tggatgcaga tattggactt
    26281 agaaacttag atgtagtaat gggtcttgaa aatcgaatag tttatgacat agtagatgta
    26341 gttgaaggaa cttgtaggct taagcaagct ctaataaaag acaaaagatt tgataatcta
    26401 tatttattac cagcagcaca aactagagat aaaaatgctg tctcagttga gcagatgatt
    26461 gacctgtgtg agaaactaaa agaatctttc gagtatataa taatagattg tccagcaggt
    26521 attgagcaag gctttaaaaa tgcagtagct ggggcagata gagctattgt agttactaat
    26581 ccagaaatat cagcagtaag agatgctgat agaataatag gtctattaga agcaaatgaa
    26641 ataaaagaga taagattagt tataaataga attagaaatg atatggttaa gcgtggagac
    26701 atgatggata aacaagatat aatagaaata ttagcaatag atttgttagg tcttgttcct
    26761 gatgatgaaa gcataattat atcaacaaat aaaggagagc cagctatact tgattctaag
    26821 tcacttgctg gtcaagcata caaaaatatc gcaaaaagga tactaaatga agaagttcct
    26881 ctacttgatc ttgaagttga agatggattc tttggtagac ttaaaaaaat gtttagcatg
    26941 gctaagtagc aaaggaggat acatctgtgt tagatttatt tagagttttt tctaacgagg
    27001 ctaaaactag taaatctgtt gctaaagaga ggttaaagct agttttagtt catgatagag
    27061 tagattgttc accacaactt ttagagatga taaaaacaga cattttaaaa gtaatagcta
    27121 attatgcaga aatagaagat gatggtcttg aaataaaaat gtctaaatgc agaggtgagc
    27181 atgatgacaa acctgtatct gcattagttg ctaatatacc actaaagaat ataaaagata
    27241 gatgtatgta attagtgctt aaaaggggga tatccttatt ggtatatgcc ccttttattt
    27301 aattatatgt ttaaagatag aaagacggtg attctttgaa aaaaatcaat attaattata
    27361 agtcgacaat taagttgata aaacagttag actggaaact tatagttaca gtattagcta
    27421 tctttatttt tgggcttgtt atacttagta gtgcaacaca tgctaattca acaggttcat
    27481 ataatcaatt gattaaacaa ggattagctt ttgtacttgg gataggaatg attatagtca
    27541 tattattttt tgactataac cttctaggca ggtattataa agcgctttat ataataagtc
    27601 ttattttgtt agccatagtt ttattacctg gaattgggac agtaaaaggt ggagctaggt
    27661 cgtggattaa cttaggacca cttgacttgc agacatctga aatagtcaaa ttaacatttg
    27721 tactaagtta tgcaaagata cttgaatcca aaaaggataa attaaatacg ttaaaggaag
    27781 ttatgcctgt tgtagtttat tctcttccat ttatcggatt attgattgca caaccagact
    27841 tgggaacagg aattgtattt tgttgtatga tatttgcaat gctgttcaca gcaggtctta
    27901 gctcaaagtt gataaaaaga ggaataatca tactattagt gtcaatgcca ctgatgtatt
    27961 taatgatggc agaccatcaa aaggtaagaa tagaagcttt tctaaatcca gaagatgtaa
    28021 ctctaaaagg aaactatcaa gttatgcaat ctctgattgc aattggttct ggaggcgtaa
    28081 ctggtaaagg actttacaat ggaagtcaaa atcaagaaga ttttttacct gtacaagata
    28141 gtgattttat atttgctgtt gtaggtgaag aacttggtgt aataggaatg gcagtgttga
    28201 taatcttatt tatgatattc ctattaaggc ttttagcaat tgcaagagat gcaaaagatt
    28261 tttatggaac cctcatagtg gttggtgtta tgggaatgtt tggttaccag ataatccaaa
    28321 atataggtat gacggttgcg ttaataccag taactggagt aacattacct tttgtaagtt
    28381 atggaggaag ttcgttattg acatctttag caaacttagg attagtacta aatgtgtgta
    28441 tgagaagaaa gaaaataaat ttctaaattt aaatacatct aaacataaat tgaggagatt
    28501 agatatatga atatagcatt agtagcacat gaccaaatga aaaacactat ggtcggattt
    28561 tgtataggtt atgaatcaat tttaaaaaag tatggactat atgcaactgg aactacagga
    28621 aaaagaataa tggatgaaac tgaattaaat ataaatagat tagcatctgg tcctttaggt
    28681 ggagaccaac aaataggttc tttaatagta acacaagaaa tagatttagt tattttttta
    28741 agagacccat tgacatctca ggctcatgaa acagatatac aagcgttaat aagactttgt
    28801 gatgtttatc atgttccaat agctacaaat ctagcttcag cagaaatatt tataaaagct
    28861 cttgatagag gagaactatc atggagagaa gttagaaaaa gcaaaagtca acgtatttaa
    28921 aaaatattaa tattacagtc taaagaaaca gatatataag tgtttaatgc cctagtcttt
    28981 ctgttggaat aaatctagta cctgtagaaa tcttatgaaa gatttaaatg atgaagagct
    29041 aaatttgaga ttaaataatt aggaaataga ttatagatag agtagtagaa tttaaactat
    29101 tactctattt taataatatg aaatagaatc aagtctatta ttatatatat ttaaacttat
    29161 aggtaatact atttcaacca agtaaatgtt gttatgacaa cattaataaa acaaaaattt
    29221 atttttaaaa tttgataaat agctgtatat tagtatttat agattaaata ttaatataca
    29281 gctatttatt atttttaagt ctattgtaga atataaacct tgcaatatag agtactatgt
    29341 aatattatag taaatataag tactatacat tatagagtag taggtgaaaa ggcggtggta
    29401 cataatgcag ttaaataaag aagtgttaaa aggttatatc gatatattaa ttgtttctat
    29461 attagaaaag aaagattgtt atggatatga aattgcaaag caagtaagag aaagaagtga
    29521 gtttgaatta aaagaaggaa caatgtatct tgcacttaaa agaatggaat ctaaaaattt
    29581 aattaaatct tattatagca atgaacaaag ttcaggtgga agaagaaaat attataattt
    29641 aactaatgaa ggtaaagatt ttttagaaat aaagaaacaa gaatggagat ttattaaaaa
    29701 ggttatgaat caatttttgg gagaggtgga ttaatttatg aaactaatag atgaatattt
    29761 agataaacta tataaaaaat gtgacaataa aagtactata gaattaaaac aggaaatgag
    29821 atgccatctt atagaatctg caaatgaatt taaattagaa ggattagatg aagaagaagc
    29881 atgtaaaaaa gcaattgaaa gatttgatga tggtgatgaa atgcagtatg aattatgtaa
    29941 tataataaaa gaactatctt tatcattgga taggcataaa tcaattgtaa tggggtttaa
    30001 aaaagtatta ggttacataa gtataattgc atttttaatt tctggattta tgtggtatta
    30061 caataatagt ttacaacata atatgtataa tttaggaaaa gaacttgatg gagaaataaa
    30121 gcaattggca gaaagacatg atatgacaaa gattggtgaa tataagttag aacttgaaaa
    30181 aatacttgat aaagataagt atagtaaagt caaagctctt agactatatg taatagatat
    30241 gaaagatgga aacactaatt taagttcttc tggattgaat gctaatatgg tatatgagag
    30301 ggaagcagat tataataaca taagtaattt tatacagcat ttaggatata atggaaaaga
    30361 ttttttagac aagaatggga atatagtaaa tccagatatt ttcttagaat atttctttta
    30421 ttttgaatcg gagatgttaa ttccagtagc atttgcattt ggattattat gtataatagc
    30481 gtattttata ttaagattta aaatttcttt aataaaaaat aataattaaa taattcacca
    30541 aataatctta atttgtagag taaataagat gtttcaaaat aaaatttgaa tcatcttatt
    30601 ttttattgtt tgttatactc ttttctttta tatttctcca tagtggattg tatctataca
    30661 taatcacata ttttgatata attatacttt ccaaaaaatg tattgaatat acaaattata
    30721 atctgtataa tgttgttagg ggagaatgta tgaagtgtat acaattttaa ttttaaatgt
    30781 attattgaca attctcctca ctctcgccat taccaataat tggaaaattg acatacctgt
    30841 tacagaccaa catttttgaa tatattaatt attatcattt tattgtgatg atatcaaaaa
    30901 tgcaggtttg tgagttggat tgtatacgaa ctatatagga aatatagaac agattttcca
    30961 agaaatctaa atattctatg accacgtgaa ttgctatgca tacatgttga taatccatat
    31021 tttaaatcta aaatgaactt aattaatggt agggaattga aagtaaaata tattataaag
    31081 gagaatgcta atatgaaaaa aatcaggaaa ttagctacta aactattgat tgcaatgatg
    31141 gttttaacct catgtatgag tgtaacagtt tatggtatga cagctaaaga tgttactgca
    31201 aagacaccga aaagttatgt agctggaacc aacagtatat atggtccaaa gttatcacaa
    31261 gctcaactta attcggtagc acaagcaaca gcagacttta tgaacaagaa aattacaaag
    31321 aacatgacca cagatgctaa gattagggtt gcttataatc acattaaaaa caacacaaca
    31381 tatgttaact ggaacgcagc agagggagcc aatactgctt atacattggt tactaaaaaa
    31441 ggtgcttgtt ctggaatgtg taggagcttg aaagctttat gtgatgctat gggaattgaa
    31501 tgttattatg tacatagtac aacaaatgac catcagtgga atcttattcg ttttgaggat
    31561 ggacgtttgt atcatgtaga tattgatgcc aacaaatcag caggagcaga catcatatac
    31621 aagagtctta ctcacccatt accatttgac aagaaagcat atccagctgt tggcgaagta
    31681 gtaaagacac agaatccaac gccaactgtt aagaaaccta cgccagctaa gccttctgcg
    31741 ccagttggac ctgggaatca cactatgata gcagatcaac aaggggcaat acttgatgaa
    31801 gtttatggaa ataatctacg tccaaaaatt gaaatgtatg agggagttcc gtgtagttta
    31861 atcaactcat caataaatac aattaagttt acaagcaaaa attcaaattt agacccatca
    31921 aagcatacag ttactgtaat agggtttatg aatacaccat atggtatggt tgcattagct
    31981 ccagatggtg tacctgatga gtatcctttt gaatatgata agccattttc tttagatttt
    32041 actggtaaga acctttctgc agcagaatta tcaaaaaagt gtaaagaaaa taatgtaatt
    32101 attaaagtgg aaattggtga aacggcatct aattctggaa gagggatggt agcatacgct
    32161 agatatgtga attaatacaa atatactagt gtacaaatag aacatattat aaataatgca
    32221 ccctcgcaca aagggtgcat ttatcattat atcagtcatt ttagaaaata aaaaaggagt
    32281 tgaacttaaa attagattag aaaaattaat tttgagacaa ctccatttta atttattttt
    32341 aaatataaaa tcatttattt taaataatat tatgtttgat taatggctat ttaactaatt
    32401 ttgcaaattc tgcaccaaat gccatacact cttttaatgt ctcttcacta gggaagaatt
    32461 ttttcttaag agattctaca ggcattttga atgacataga tttaagtaaa gtctcagcca
    32521 tagttatacc ttcaccactc caaccaaatg aaccaaatac tcctgctatc ttgccttggt
    32581 tagccattgg gtctataaca gagaataaat cccacatagg tttaaccata gttttgttta
    32641 tagttggaga acctagtaat attacttttg acactactaa tgtatcgtgc atttctgtta
    32701 atgtcatatc ttctaaatca tataattcag cctttgcacc ttctttatca agtccttctt
    32761 ttatcttttt agccatctct aaagtattac tataagctga taagtagaat atagaaactt
    32821 gattttgatt agttgtatta acagcttctg tagaccactc aacatatctt ttaacagcag
    32881 ccattgggtc tttagtaagc attggaccat gagaagtcaa tatagtatca aattctatgt
    32941 ttagtcctac caccttatct acagcactta aaacatgttt agcaaatggc ttaactatac
    33001 aatcatagta gtgtttagct gattttagat aatcttcact attcactacc tctgcatcaa
    33061 cacttgcaaa gtgacaacca aatgcatcac aagttaaaag agtcttgtcc tcctcaatgt
    33121 aagtaaacat tgtatcaacc caatgtaaga atggagcagt tataaatcta agatttcttt
    33181 taccgatatt taaaatttca ccatctttta ttacatgaca gttaaaaggt ctatttattt
    33241 gaccatctaa gtatagttta gcagctttag tacaataaac ttctatatta ggatttatat
    33301 ctaataagta ttttaaacta cctgcatggt ctggttctgt gtgatgaatt acaacatagt
    33361 caattttagc tatatcagta acctctgata aattacttaa aaactcatct ttaaaatttg
    33421 cctttacagt gtcaaataaa accgtttttt cgtccttgat taaataagag ttgtaagttg
    33481 tcccaaactc agtttccatt attatgtcga atacttttaa accttcatct actactccag
    33541 taaagtagat atcttttttt acttcaaaaa ctttactcat ttttaaccct cctaaaattt
    33601 atcattatat aagtaacata ccccattttg aggtgaatga aacaaaagat gtcatgataa
    33661 gtcatgtaaa acaatagttt tatgtttata atatatcata ctttgtaaaa aaattgtatt
    33721 tcaataaaaa tttaatagaa aatattctta aatattttta gctattctat tttgtgaata
    33781 atatgtattt taaattcaat attttagtta tatttttgat agaaaatatt ttatcgtgta
    33841 taatattact tataaataag aataaaaata aagagcttgt ttggaggaat tttaataaat
    33901 gaataaagta gacttgaaaa aaatcttaaa aaaagtagaa aagcctgcta gatatcttgg
    33961 aaatgaaata aattcaattc ataaagatac tagtaatagt gaattgataa gatatgcaca
    34021 ttgctttcca gatttatatg aagttggtat gagtcactta gggagtcata tattatatga
    34081 tgtaataaat aaggatgaag atgtattttg tgagagagtg tattctcctg cagttgatat
    34141 ggaaaatata atgagagaaa aaagtatacc attatttgca ttagagtcaa gagagcctat
    34201 aacaaacttt gattttgtca cttttacact tcaatatgaa ctttcataca ctaatatctt
    34261 aaatatatta gatttagcca atataccaat attaaaagaa gaaagaactt tggaagaccc
    34321 atttataatg gttggtggtc cttgtgctta taattccgaa ccattagcag attttgtaga
    34381 tgtagtaata ttaggtgaag gtgaagaggt taatttagaa gtagtaaatg agtataaaga
    34441 atggaagaaa aataaaacga ctagagaaga ttttttatat aaaatatcta gcatagaagg
    34501 agtatacata ccaagttttt atgatgtcaa gtacaatgaa gatggtacag ttcaaagtgt
    34561 aactcctaat agagaaggga ttactaaaaa tcctactaaa aggataataa aagatgttga
    34621 gactgtagat tatccagaaa aactaatagt accttatata gatactgttc atgatagaat
    34681 agtactagaa ttatttagag gatgtactag aggatgtaga ttctgtcaag caggtatgat
    34741 atacagacca ataagagaaa agagtgttaa aagacttaaa gaaatattag ataaattagt
    34801 taagaatact ggatatgatg aaatatcatt atcatcactt agtacgagtg attatagtaa
    34861 gttatcagag ttgactgatt atttggtaga tgaatatgca tctaataata ttgggatttc
    34921 gcttccatcc cttagattag ataatttttc aatggaaatt gctgataaaa tacaacaagt
    34981 aagaaaatct ggacttactt ttgctccaga agcaggaact caaagactta gagatgttat
    35041 aaataaaggt gttacagaag aagatttgga aaatgcaaca gaaagagctt ttgagatggg
    35101 gtggaatagt gttaagttgt attttatgat tggtttacca actgagactt atgaagattt
    35161 agatggtata gctaaattag cttataaagt tattgatatt tatagaaaag taaatggagg
    35221 aaagctaaag agaagtttta gtgttacagt gagtacttct acatttgtac caaagccatt
    35281 tacacctttc caatggcatg gtcaagatac tacagaagaa gttattaata agcagagaca
    35341 tttagtaaat aaattaagaa ataacaatat aaaatacaat tatcatgatt caaagactag
    35401 tttgatggaa gcagtagtag ctagaggcga tagaagaata ggaaaagtta tatatgatgc
    35461 atttagacta ggtgctaaat ttgatggatg ggcagaacat tttaatttag atatatggaa
    35521 agaagctatg gaaaagaata atctttctat agatttttat gcacatagaa atagaaacta
    35581 tgaagaggta tttccttggg accatataga tgtaggtatt agtaaaaaat tcttaattag
    35641 agaagacgaa aatgctaaaa aagaaaaaat cacttctgat tgtagacata actgcaatgg
    35701 ttgtggaata aatatacatg atataggaag ggggctttgc taatatgagt aagataataa
    35761 gagtaaaatt taaaaaggaa ggcgacatga tatatatatc acatcttgac cttcagagat
    35821 tgttacaaag agcgtttaga agagctgaaa taaacttatc acattctcaa ggatttaatc
    35881 ctcaccctaa aatgagttat ggcaatgctt tggctttagg gacagaaagt caaggtgagt
    35941 atgtagatat tgaaatagaa gaagatgatt tatcagtaga ggaattttta aataaagtat
    36001 caattcaact tccagatggg atagatttta tcaaagcaaa agaaattgat agacaaacac
    36061 cttctctttc ttctgtgata gactatggag aatatttgtt taatatagat ttgaaaagac
    36121 ctatgacgaa agagtttgta aaaagaaaag taattgactt tatgaataat aaagaaataa
    36181 tcataacaaa aaagaataaa aaaggtaaga tggttgaagt agatataaga cctatgataa
    36241 gaacctttga tgttttaaat ttagaagatg aacatattac tcttactgct actatagcta
    36301 caggctcaaa aactaattta aatactaata tacttatacc aaagatatta gagatgtttg
    36361 aacttgatat tgacccatta gatgttgata tattgagaag agatttatat gtattagaag
    36421 atggggaatt agtgactcct atgtaagctc gagaggtgaa gttgtgaaaa agatagttat
    36481 tgagtcccta atagggtccc agaaaacagc agtacttgaa gatgaaagat taacagaact
    36541 ttttgttgaa gacaatttaa ataagaagac tgtatccaat atatatcgtg gaatcgtaaa
    36601 aaaagttata ccaggaatag aagcttgttt tgtagatatt ggatttaaga aacttgctta
    36661 tttacaatta aaaaagggaa gtactataaa atctggtcag gatattttgg ttcagataaa
    36721 taaagaagaa ataggaacta aaggcgcaaa attaaacact gagattagta tttctggaag
    36781 gtatattgtc tatatacctt ccaatgatag aactacaata tcaaataaaa ttactgatga
    36841 aaaagaacga tttagattaa aaaaaattac taaggcagta aataaggaaa atctaggatt
    36901 aataataagg actgaggctc aaggttgcac tcatgatgaa ataaagaaag atatagaaga
    36961 gttgaaatta aagtatgaaa acattttaaa agaatataaa cttggtatag gtccgaaatt
    37021 attatataag tctttagatt ttgcaactaa atatgtaaaa gataatgtga atgatgatat
    37081 tgaatctata attaccaata gttatgataa gtactctgaa cttaagtcta ttttaagagg
    37141 tatagataag acttatgttg ataaactgtg tcttgaggaa aatagagatg tatttgattt
    37201 atacagaata gaatctaaaa tagaaaaatt attaaataaa aaagtatggt taaagagtgg
    37261 aggttattta ataattgaaa aaacagaagc tcttacggtt atagatgtaa atacgggtaa
    37321 gtttattgga actggtaaac ttgatgaaac cgtttataaa actaatcttg aagctgcaaa
    37381 agaaattgta agacagctta gaattagaga catagctggg ataataataa tcgattttat
    37441 tgatatgcat aaaaagaagc atcaaaagga gattttacac atcctagaag aagaatttaa
    37501 taaagataaa agaaaagctg aagtattagg tatgacaaaa cttggtttag tagaagttgc
    37561 taggcgtaga gaaaaagaat ccattgataa gtattattta atgtcttgtc catgttgtga
    37621 tggtgaacaa acaataaaat ctgtacatta tatactagat agtattgaaa aagaaattat
    37681 gagaataagt gaacatactg tttacaaaaa cataatggta gaatttaatg attttatttt
    37741 tgaacaaata aaagaatatt atatggatat aattgataaa attggtgaaa aatataatat
    37801 aaagatttct ttaaacgcaa atagtacatt aaagcacaat aaaaccaatg ttattttcga
    37861 taaaatagtt gacaataaaa tgtaaatatt ttataattaa taattgtaat gccgcacagt
    37921 aaaggtttaa atcttcaaat gaagtacctt gtactggcga gtttgaggcc gaggaggtgt
    37981 atttaatgta cgctatagta aagactggtg gaaagcaata taaagtttct gaaggtgatg
    38041 tattattcgt tgaaaaatta gaagctaacg caggtgatgt tgttacttta aacgaagtat
    38101 tagcttgctc taaagatgga gaattaaaat taggttctcc agtagtagaa ggagcatctg
    38161 ttcaagctaa agttgttgaa caaggtaaag ctaagaaagt tatagttttc aagtataaag
    38221 caaaaaaaga ttacagaaga aaacaaggac atcgtcaatc atacactaag atagttgtag
    38281 aaaaaataaa cgcttagtta ttggttaagt tatgataaaa gttaaatatt attataatga
    38341 tgatttcttg ttgcaaggtt tttgtttaaa gggacatgct gattttgctg aaataggcta
    38401 tgatatagta tgtgctgcag ttacatctaa tgctattgca gttataaatt ctttagacaa
    38461 attacaaaag attgaatttg aaaaagtagt aggagaagaa ggtcatatag agtgtatagt
    38521 caaagataca catgtaaaag acgcacaatt attactaaat cactttcaat tagcggttaa
    38581 agaaattaaa cgggaatacc caaaaaatat aaaaatttta aaaaagtagg gggtgactcc
    38641 atatgttaaa catgaattta caattattag catctaaaaa aggggtaggt tcttctaaaa
    38701 atggtagaga ttctatatct aaaagattag gtgttaagag attcgatggt caattagtaa
    38761 ctgctggaag tataatagtt agacaaagag gaactaaaat acatccagga actaatgttg
    38821 gaaaaggtag tgatgatact ttatttgcat tagttgatgg tactgttaaa ttcgaaagaa
    38881 aagacaagaa gagaaaaaaa gttagtatat atccagtagc tatagctgag taatatacat
    38941 aaaaggagtg ttgaggcact cctttttctt ataatactga tgaatttata atggtatttt
    39001 tggtaatgct aattaaaagt ttttaattaa gtaggtgaag aatttgttta tagataaggc
    39061 aagaatattt gttaaggctg gaaatggtgg aaatggttcg gtagcattta gaagagaaaa
    39121 atacgttcca gctggtggtc cagacggtgg agatggtggt cgtggagcaa gcatcatatt
    39181 tgaagttgat ttggggctta gaactctaat ggactttaag taccagaaaa aatatcaagc
    39241 tcaaaatggt ggcgacggaa gcaaaggaaa aagagctggt aaaaatggag aaaatttagt
    39301 attaaaagta ccagcaggta cagtaataag agatgaggct acaggtcttg tattagcaga
    39361 tttaaaaaaa gaaggagata cagctatagt tgcaaaaggt ggtataggtg gtaaaggtaa
    39421 ccaacacttt gcaaatgcag taagacaagc tccagctttt gcaaaatctg gtacagatgg
    39481 agaagaaaga tggataactc ttgaacttaa aatgatagca gatgttggtc ttttaggatt
    39541 tccaaatgta ggtaaatcaa catttctatc agttgtaact aaagctaaac ccaaaattgc
    39601 aaactatcat tttacaacac ttacaccaaa tctaggtgtt gttcaaacta agtttggaga
    39661 tagttttgta cttgcagata tacctggaat tatagagggt gcttctgaag gcataggatt
    39721 aggtcatgag ttcttaagac atgttgagag aacaaaggta ttgatacata ttgtagatat
    39781 atctggatta gagggaagag accctattga agattttgac aagataaatg atgagcttaa
    39841 attgtacaat gaaaagcttt caaagagacc acaagttgta gttgcaaata aatttgatat
    39901 attagaagat gagagtaagt ttgagaagtt taagagtgaa ttagaaggca gaggatatac
    39961 agtatttaaa atgtcagcag caactcgtca aggcatagat gaagtaatag cctatgtttc
    40021 taaaatgcta aaagaagtag aagatgtgga gctagtttca gaagaagaaa tgtatagacc
    40081 agaacttgat attggtactg aagaagagtt aagtatagat attgaagatg gtgtgtatgt
    40141 agttacaggt aaagcactta gaagaataat gtattctgta aattttgatg atatggaatc
    40201 ccttcaatat ttccaaaaag ctatggagag tcaaggtgtt tttgataggc ttagagaaat
    40261 gggaatagaa gatggagatg tggttaaaat ttatgaatta gaatttgagt tttacaatta
    40321 atttaaatat agattataga taatttaagt atagaattat agaggtgaga atatgttaaa
    40381 aggaaaacaa agggcgtatt taagatcttt ggcaaatact ttaaagccta ctacacaaat
    40441 tggtaaagaa ggtgttacag agagtttctt agagcaatta gatggtatgt taagaactag
    40501 agaattagta aaagttacta tattagaaaa tgcaggtctt gatactaaag aaactgctaa
    40561 tgcagtatgt gaagctttaa gagctgaatt tgtgcaggct attggtttta aatttactat
    40621 ttacaagagg aatattgaag agcctaaaat actttttcca ggacatgaac aggcgaaagc
    40681 aaagactaaa aataataatg ttacaaaaaa gggtaaacca actaaaagag ctgttagata
    40741 atagtttata tccttttagg gaaataagtt aaaaaaatag tgacaattgg aagaaattgt
    40801 attatactat taaacataaa tttaaattag caatcttatc aagagtggca gagggactgg
    40861 ccctgtgaag cccggcaacc agtatattta attatacatt ggtgctaaat cctgcagcat
    40921 ttatgctgaa agatgagtat aaacccgtca tatggcttga gtttatactc aagctttttt
    40981 tattggtata tttatatata taaataattt aatataatca aaatgatggg ggtatgttac
    41041 agtgaagata gcaatattag ggtatggaac agtaggaagt ggattaattg atttaataga
    41101 aaataatact tcaaaaagag atatagaagt tgttggaatt ttagttaaaa ataaagaaaa
    41161 acacaagtat aagaagtact ttgataagat aactactgat atagaagata tatttgacaa
    41221 ggatattgat atactagttg aagtaattgg aggattaaat ccagcttttg attatgtaac
    41281 aagagcgtta aataaaaaaa tacatgtagt gacagctaat aaagatttac tggctgaaaa
    41341 aggtagtgac cttatagaat tagctaactt aaataatgtt agcatcaagt ttgaagcatc
    41401 tgtggcaggt ggtattccag ttttaaaacc attgatagaa tcactggaag gcaacaatat
    41461 aaagagcata aacgctattt taaatgggac ttgtaatttt atattatcaa agatgtatga
    41521 tgaaaattta ccgtatgatg tcgctttaaa acaggcacaa gagttgggat ttgcagaagt
    41581 taatccagat gcagatgttt taggatatga tagtgctaga aagttatcta tattgtctac
    41641 tttatcttat ggaaaaatag tatattggaa agatttatta ttagaaggaa taacttcaat
    41701 agatgaaaaa gatatagaat atgcaaaaaa attaaattgt aagataaaat tggttgctag
    41761 aagtaagtat gaatcaggag aagtaagtgg atttgttaga ccagcattag tggacaataa
    41821 taatatgctt tcaaagatag ataatgagtt caacgtagtt attttagtag gtgattcagt
    41881 aggtgaactt tcttttgtag gtaagggagc tggaagaggt gcaacaggaa gtgctgttta
    41941 tgcagatatt atagatataa tagataatag aagttcaaat ataaagtctt tttcaaaagg
    42001 gaaattggat ttaagtggac ttatagaaga tgagtgtagt gcagtagtta gatttggtgg
    42061 gtataataat aaagatggaa ttttagaatg tttagataaa tatgtagata gttatgatat
    42121 tatagatgat gaagaattgg cgatttttgt aagtgcaaag tcagaacatg agattgatag
    42181 agttttggaa attgtaaaaa gaaataatta cagtgaatca gctaaaaaat tattgaagat
    42241 agattaatat gtttatgcaa gaattatgaa aatttaatag ggtgtctcaa aatagagaga
    42301 taagcctttt ttccagtgtt gaaataataa aaatatattt gattttataa aaaaagagtg
    42361 tcttatgaac taaaaagttc attttgagac actctttttt aatatctggt gaattaataa
    42421 gataatagaa aaatgtatac gttataatta accaataaag aaattttcac tagcttcatt
    42481 aatagcttgc tgagttaccc ttatatatcg ctgtgtagtg gctacagagc tgtgatttaa
    42541 aagttcttta accaactcta aattattgtt actcttttca aattgagtag tagcatagag
    42601 ctttctaaag ctatgtgtac ttatattata cagatttaag tgtttacaaa ttatcctcaa
    42661 ctgtttttga atagcctttg tctttatatt tactaagtag tcatttgatt taagattata
    42721 ctttcttgca tgttcattta tggactcaat tatattttta ttaactggtc tatactgtaa
    42781 tttgcccgtc tttttttcat ttatttccaa catattacct ttaaaattac ctatctttaa
    42841 tcttaaaata tctgatattc ttagacctaa attagcttct aacattagtg ccaatgcaac
    42901 tttaggattt tttctaaatc ttttttcaac gccattttca ctataagtaa agccagtatg
    42961 taatagttcc attatctttt tatattcgtc tatttctata ggtcttgttg ccattttcaa
    43021 ccactcctct tagtttgatt tggtaagttt aggtcggtat ttattatata atataaatga
    43081 aatattatat aatggaaaaa tttcccagta aacttataca agtgtgatat atgtgattaa
    43141 ggagaatttt ttgaaaaata tttctaataa aattattaaa ttgtgatgaa ataactatgc
    43201 tgttaaacta attaaaaagg ttattaaaat tatgacaaat ataataaaaa aatggaagta
    43261 tttagttaag ttttattagt tttattttta gttttatttt tttcgtgaaa atttcttagt
    43321 gaaaaaagta tttttatatc gcattagttt cccatttgta gataaagttt tcatatgttt
    43381 ttttgcaata aatacggctg agattagata aattattact acaatttgaa gaattgtatc
    43441 ataatagcta aggtaataaa accagattaa attattatat ataacgttgt atgtatcagc
    43501 aaagctagaa accaacacag cagagtttgc aattggataa agtataaata taaagatagc
    43561 agctaaaact ccatgcgcaa atttacttag tatgtataat tttaaatcta tgtccgtttt
    43621 tgatagaaat gtacaacatt gagctaggat ggataatcca ctaaatccta tcaaaaaact
    43681 agctaaagat gctcttaaaa tttctggaga acttgttata ctagatactt tattgcatcc
    43741 tatagtaatt tcaaatagac cacttacaaa tgcttgacag agttctttac taacacctaa
    43801 aaatgaaaga ggcaaatata taactgatgc tattaatgag attacattaa aaagagatag
    43861 tattttaaat actactgaaa aaacaattac aaatccacca actgtaagta atgtgtttac
    43921 tccattaaat acagcatttc caaataaaac aaaaaaacca ttcccacttg aatttctaat
    43981 gttgattaca ttttttatat ttgtttttaa atccaatttt gtctttggaa ggttttcatt
    44041 accataattt ctaaagaaaa gaccaaccaa tatagttcct agataatggc agagaatcat
    44101 taaataacct aagtttgaat tttggaacat acctgtacca accgaaccta ttatgaataa
    44161 aggtcctgat gtagaacaaa aagacacaag tctttgtgct tcatacttag aaatttgtcc
    44221 attactccta aattcagaag ctaaacttac tcctactgga taaccagata ctgtagatat
    44281 agcaaaagct aaagcgctct ttcctgaaac attaaataca aatctagtta tgggattgat
    44341 gataaaccct ataatatcca ctatttttaa atcaacaatt aagtttgctc caattataaa
    44401 tggtagtagt gatggaacta aaacattaga ccatatagaa aagccttctc ttgcagattt
    44461 tatagcttca tttgggtata ggataatccc taaaactaga actccaacta tgatagatgg
    44521 agcaaaaaaa gcaataattt tatttttttt cataatatca cctgtaaatt tgagttataa
    44581 gatattatat taaaagtaat aaaaatatat gaatgtaatg gcttaagata tataatagat
    44641 atatattaca acaacgatta tcatattgga ggaaaaaatg tttaatagta tagctagtaa
    44701 aaaagtttat gaacaagtaa ttgagcaaat acaatataaa atactaaatg gagagttaaa
    44761 aaagggggat aaattactat cagaaagaga actgtctgaa caaatgaatg tgagtagaac
    44821 ttccataagg gaagctatta gggtattaga gactatggga gtgatagaaa gcagacaggg
    44881 tgaaggaaat tttatatgta caaatataga aaaaacttta atagaacctt tatctatgat
    44941 ttttaaactt aataatggta cacttgaaga tatattagag cttagaataa tcctagaaat
    45001 tgagattgca aaacttgctt caaagagaat aacttcttct gaagttatag aattgaaaca
    45061 tataatagat gaaatgaggg tagaaactaa taaaaaagat aataatcgag tactggtatt
    45121 attagaccaa aaatttcaca gtaaactagc aacattaagt aaaaattatt taattcaaag
    45181 tctttttatg acagcatcaa aattatttga tggatttata gaagatgcaa gagaaaaaat
    45241 aatcgcagaa ccttttaatg aaaatatatt gcttaaacag catgaagcta tatacaatgc
    45301 agttgtagaa aatgatgtag aacttgcatg tgagaaagca aaggaacata tggattttat
    45361 aagtaaaaat tatagaaaaa atgaaaatta aaagtttgtt ataaaataaa ttattcttgc
    45421 aaatggtaga taatggtact attatataaa tgtaaaactg gtatgaccac ataccaattt
    45481 tatagtttat tataatggga gattaaaaat ggtaaagtta aaagtaccgt attcaaaaca
    45541 aggaatgatt ttagaaatac ctgatgaacg ttttctcggt gttttggagt caaaatcgga
    45601 taattatata gaacataaga ctcaagaaga gatagttgaa gaatctatga ataatcctat
    45661 aggctctaat tctctggaag aactggttaa ggggaaaaag aatgtagtca taattacaag
    45721 tgaccataca agacctgtac caagtagaat aactatgcca attatattaa gaaggattag
    45781 gaatgttaat ccagaaattg atgtgaagat tatagtggca actggatttc ataggccaag
    45841 tacaagggaa gagctaattt ataaaatggg agaagaaata gtagataatg aagatatagt
    45901 tatgcatata tctactgatg ataaatctat gtgtaaagta gggatattgc cttcaggtgg
    45961 agatttatat ataaacaaac tagcatatga ggcagaactt ttgatagcag aaggttttat
    46021 agaacctcat ttctttgcag gtttttcagg tggcagaaaa agcgtacttc caggaatcgc
    46081 atctgcaaag actataatgt acaatcactg tagtgacttt atagatagtg ataattcaag
    46141 aactggaaaa ttgaataata atcctattca tgaggatatg gtatatgcag ctaaagttgc
    46201 aaaattagca ttcatattga atgttgtaat agataaagat aaaaaaataa ttgcttcctt
    46261 tgctggtgat gtcgaaaaag cacatactaa aggatgtaga tttgtaacag agttatcaaa
    46321 agtaaattct ataaaatctg atattgttgt aactacaaat ggtggatatc cactagacca
    46381 aaatatttat caatctgtta aaggaatgac agcagctgaa gctacttgta aagacggtgg
    46441 agtaataata atgatttcag catgtaatga tggtcatgga ggtcaatctt tttatgaaaa
    46501 tgtagcaaat gcagattctc caaaacaact tttagataaa attagaagtg tcgatagagt
    46561 agatacgatt ccagaccaat gggaatttca gatactagcg agaatattat ctaaatatac
    46621 tgtgataatg gtaacagata agtgtaatcc tgaaatgata aaaaaaatgc atatgaaaca
    46681 tgcatttaac tttaaagaag ctttagaaat ggcgaatagt atagttggta aggagagcaa
    46741 agtagtagtt atacctgatg gagtatcagt tatagttaaa taaaaaagga gtgtacttta
    46801 tggaaatttt gacatgtatt aaacaagttc ctggaacgac ttcagtagaa gttgatgaaa
    46861 ctacaggagt actaaaaaga gatggagtag attcaaaaat gaatccctat gacctatatg
    46921 ctttagaaac agctcttaga ataaaggaag ataagaaagc gaatctaaaa gtacttagca
    46981 tgggtcctcc tcaggcaaag aaggtaattg aagaaagttt tatgatggga gctgatgaag
    47041 gagcattaat aagtgatagg agatttggag gagcagatgt attggctact tcctatacca
    47101 tatcacaagg tattaagaag atgggaaagg tagacctaat aatttgtgga aaacaaacta
    47161 ctgatggtga tacagcacag gtgggacctg aagtagctga atttttagat atacctcatg
    47221 ttactaacgt tacaaaatta attgaagtta aagatgaaag tatagttgtt gaaatagata
    47281 tgccaaatga tttacaagta tgtgagattg aatatccatg tttaataact gtagaaaaag
    47341 acatttttca accaagacta ccttcattta aattaaaact taatactaaa gatagagaaa
    47401 tacctgtata tagcttagat gactttgaag ataaaaatga aaataactat ggacttaatg
    47461 gttctcctac tcaagttgta agaatatttc ctccaaagcc aaatacagat aaaaatattg
    47521 taagaggaaa tgcagatgaa ctaagttttg cacttgtaaa taaattagag gaattaaagt
    47581 tggtgtaagg gggagtaata aatgtcaaag atagttgtaa atcaagataa aataactgac
    47641 ttaaagagaa ttttagaaat atgtcctttt ggggctatag aagaaaagtc aggtatagta
    47701 gaaataagtg ctggctgtaa gatgtgcaag ttgtgtgtca aaagtggacc aaaaggcgca
    47761 tttgaattta tagagagtag taaagtacaa attaataaag atgaatggag gggaattgca
    47821 gtatatgttg aacatcataa tggaaatata catcctgtaa cgtacgagct tataggtaaa
    47881 gctcgtgaga tggcatcaaa aataaaacaa cctgtgtatt gtgtatttgt aggaaaagac
    47941 ataaaagata agtgcagtaa tttgatttct tatggggtgg atgaagtatt tgtatatgat
    48001 gaagatgaat ttaaagattt taggatagaa ccatattcaa aagcaataga aaactttata
    48061 gataaaataa aaccaacgat agtattagtt ggtggaacta cattaggtag gtcattagct
    48121 ccaagacttg ccgctagatt tagaacaggt cttacagcag attgtacaat acttgatatt
    48181 caatcaaata ctgatttaga ccaaataaga ccagcatttg gtggaaatat aatggctcat
    48241 ataaacacac ctaataatag accacaattt gccactgtaa gatataaaat attttcagca
    48301 ccagaaaaaa tagagaatac tacaggaaag ataactttat gtaaagtaga aaaaaaagaa
    48361 ctaaaatcta aaattaaggt actgagtgta aaagaaaaaa ataaagaagt tggtctggaa
    48421 gaagctgaag tgatagttgt ggcaagtaga gcaataaaaa aacaagaaga tatggagatg
    48481 atatataaat tagctgataa gttaaacgct caagtggcag gaacaagacc tgtaatagaa
    48541 gcaggttgga tagatgctaa aaaacaaata ggtcttagtg gtagaactgt aaaacctaaa
    48601 ttgataataa cttgtggtgt atctggagct gttcaatttg ttgcagggat gcagggtgct
    48661 gactatatag tagcaataaa taaagatgat aaagcgccta tacttgatgt agcacattta
    48721 gctttaatag gtgatattta tgatattatt cctaaattga ttgaaaaaat agaaaataac
    48781 aaagataata atcaaaaata tatggcatca gcagctaaat agggggatag aaatgtataa
    48841 attaatcgat aaaaaagata tagatttttt aatagatact tgtggagaag aaaatgtatt
    48901 agttggaagt gatataaatg aagatttttc acatgatgaa cttggaggga ttgagaagta
    48961 ccctgaagtt ttagtaaatg tacttgaaac tgaacaagta tctaaaataa tgaaatacgc
    49021 atataaaaat aatataccag tcacaccaag aggtcaaggt actggtcttg taggtgctgc
    49081 tgttgccata aatggtggaa taatgattaa tctatgtaaa atgaataaaa tacttgaagt
    49141 tgattatgaa aatttaacat tgacagtaga accaggtgtt ttattgatga caataggtca
    49201 atatgttcaa gatagagatt tattttaccc acctgaccca ggtgaaaaaa gtgctacaat
    49261 tgcaggtaat ataaacacta atgcaggtgg tatgagagca gtaaaatatg gcgttacaag
    49321 ggattatgta agaggtcttg aagttgtact accaaatgga gaaattataa atgtaggtgg
    49381 aaaggtggtt aaaaatagtt ctggatatag tataaaggat ttattagtgg gctcagaagg
    49441 tactttagga atagttacta aagctatatt aaaattgctt cctttaccaa agaaaagtat
    49501 aagtttgctt ataccatttc cagatttatc aatggcaata gaaactgttc ctaaaataat
    49561 aaaatcaaaa tcaataccaa cggctataga atttatggaa agagatgtga tattggcagc
    49621 agaagaattt ttaggtaaaa agtttccaga taatacatca gatgcttatc ttttgcttac
    49681 atttgatgga aatagcactg aagatataga aaaagaatat gaaaaagtag ctaatttatg
    49741 tttagaaaat ggtgcattag atgtatttat atcagataca caagaaagaa acgattctat
    49801 atggtcagcc agaggtgcat ttttagaagc tataaaggca tctacgacac aaatggatga
    49861 atgtgatgtc gttgttccaa gagataagat agcagagttt ataagataca ctcacgagct
    49921 tcaagacaaa ttaaaaataa gaataaaaag ttttggtcat gcaggagatg gaaatctaca
    49981 catatatatt ttaaaagatg gtatggatga taatacatgg aaaataagat taaaagaaac
    50041 ttttgactat atgtataaaa aatctagaga acttagtggt caagtttctg gtgaacatgg
    50101 cataggatat gctaaaaaag agtacttaca tgaatcaaat tcagatgctt atatgatgtt
    50161 aattaaaaat ataaagttag cttttgaccc taaaa
//
LOCUS       DACULQ010000004        50117 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.004, whole genome shotgun sequence.
ACCESSION   DACULQ010000004 DACULQ010000000
VERSION     DACULQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.004"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1193
                     /locus_tag="JA154_00905"
     CDS             <1..1193
                     /locus_tag="JA154_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAT7994414.1"
                     /translation="ILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHEKA
                     GRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFATSV
                     VITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSSYL
                     YGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAAVD
                     GGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVYGM
                     TGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVGEN
                     GKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARDTL
                     RLVK"
     gene            1286..1810
                     /locus_tag="JA154_00910"
     CDS             1286..1810
                     /locus_tag="JA154_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAT7994415.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1835..2011
                     /locus_tag="JA154_00915"
     CDS             1835..2011
                     /locus_tag="JA154_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAT7994416.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2218..2775
                     /locus_tag="JA154_00920"
     CDS             2218..2775
                     /locus_tag="JA154_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis transcriptional
                     regulator"
                     /protein_id="HAT7994417.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2786..3808
                     /locus_tag="JA154_00925"
     CDS             2786..3808
                     /locus_tag="JA154_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HAT7994418.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3834..4820
                     /locus_tag="JA154_00930"
     CDS             3834..4820
                     /locus_tag="JA154_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HAT7994419.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            4929..5858
                     /locus_tag="JA154_00935"
     CDS             4929..5858
                     /locus_tag="JA154_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HAT7994420.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5874..6824
                     /locus_tag="JA154_00940"
     CDS             5874..6824
                     /locus_tag="JA154_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[acyl-carrier-protein] S-malonyltransferase"
                     /protein_id="HAT7994421.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQVDLKYSACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQDTVPTGVGAMAAILGLDRSILENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLREAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6824..7573
                     /locus_tag="JA154_00945"
     CDS             6824..7573
                     /locus_tag="JA154_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HAT7994422.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7627..7851
                     /locus_tag="JA154_00950"
     CDS             7627..7851
                     /locus_tag="JA154_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAT7994423.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7880..9118
                     /locus_tag="JA154_00955"
     CDS             7880..9118
                     /locus_tag="JA154_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HAT7994424.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9344..11062
                     /locus_tag="JA154_00960"
     CDS             9344..11062
                     /locus_tag="JA154_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT7994425.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKHDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11093..13093
                     /locus_tag="JA154_00965"
     CDS             11093..13093
                     /locus_tag="JA154_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT7994426.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSELSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13441..13689
                     /locus_tag="JA154_00970"
     CDS             13441..13689
                     /locus_tag="JA154_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HAT7994427.1"
                     /translation="MMKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVE
                     GMNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13724..14449
                     /locus_tag="JA154_00975"
     CDS             13724..14449
                     /locus_tag="JA154_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAT7994428.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14456..15784
                     /locus_tag="JA154_00980"
     CDS             14456..15784
                     /locus_tag="JA154_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT7994429.1"
                     /translation="MGGLNMNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVA
                     SIFVYIFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCI
                     SWGALIVGFPTSLSAVWAPAGEPHIQKIIIVVMIVGLTIINLLGVSLSKIVQNVITVG
                     KLIPLILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAE
                     DMENPQKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMG
                     AGLVTFGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATML
                     ITIPIALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLI
                     AVCVGTWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYDENLKKIV"
     gene            15990..16529
                     /locus_tag="JA154_00985"
     CDS             15990..16529
                     /locus_tag="JA154_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7994430.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16618..18603
                     /locus_tag="JA154_00990"
     CDS             16618..18603
                     /locus_tag="JA154_00990"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087684.1"
                     /note="class 3; catalyzes the formation of fructose
                     6-phosphate from fructose-1,6-bisphosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 3 fructose-bisphosphatase"
                     /protein_id="HAT7994431.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18827..19795
                     /locus_tag="JA154_00995"
     CDS             18827..19795
                     /locus_tag="JA154_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HAT7994432.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19785..20306
                     /locus_tag="JA154_01000"
     CDS             19785..20306
                     /locus_tag="JA154_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HAT7994433.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20323..20520
                     /locus_tag="JA154_01005"
     CDS             20323..20520
                     /locus_tag="JA154_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HAT7994434.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20536..20922
                     /locus_tag="JA154_01010"
     CDS             20536..20922
                     /locus_tag="JA154_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HAT7994435.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21001..22212
                     /locus_tag="JA154_01015"
     CDS             21001..22212
                     /locus_tag="JA154_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HAT7994436.1"
                     /translation="MLMKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGET
                     KNSIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGL
                     KILMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLI
                     GFKDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFF
                     KNFLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLY
                     VTSFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICL
                     LPVIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVA
                     ILTSISVVSQG"
     gene            22272..22889
                     /locus_tag="JA154_01020"
     CDS             22272..22889
                     /locus_tag="JA154_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HAT7994437.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22901..23455
                     /locus_tag="JA154_01025"
     CDS             22901..23455
                     /locus_tag="JA154_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HAT7994438.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23482..24171
                     /locus_tag="JA154_01030"
     CDS             23482..24171
                     /locus_tag="JA154_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HAT7994439.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSYSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24281..24643
                     /locus_tag="JA154_01035"
     CDS             24281..24643
                     /locus_tag="JA154_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAT7994440.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24725..25234
                     /locus_tag="JA154_01040"
     CDS             24725..25234
                     /locus_tag="JA154_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen utilization protein B"
                     /protein_id="HAT7994441.1"
                     /translation="MMKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDN
                     SEHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKH
                     AKNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGIL
                     GSVVDEIGK"
     gene            25218..26288
                     /locus_tag="JA154_01045"
     CDS             25218..26288
                     /locus_tag="JA154_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HAT7994442.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26285..27490
                     /locus_tag="JA154_01050"
     CDS             26285..27490
                     /locus_tag="JA154_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAT7994443.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27492..27707
                     /locus_tag="JA154_01055"
     CDS             27492..27707
                     /locus_tag="JA154_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAT7994444.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27710..28597
                     /locus_tag="JA154_01060"
     CDS             27710..28597
                     /locus_tag="JA154_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAT7994445.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28627..29064
                     /locus_tag="JA154_01065"
     CDS             28627..29064
                     /locus_tag="JA154_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HAT7994446.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29098..30963
                     /locus_tag="JA154_01070"
     CDS             29098..30963
                     /locus_tag="JA154_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAT7994447.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31096..31908
                     /locus_tag="JA154_01075"
     CDS             31096..31908
                     /locus_tag="JA154_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HAT7994448.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            31928..33607
                     /locus_tag="JA154_01080"
     CDS             31928..33607
                     /locus_tag="JA154_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAT7994449.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33822..34688
                     /locus_tag="JA154_01085"
     CDS             33822..34688
                     /locus_tag="JA154_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994450.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDIDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34781..35260)
                     /locus_tag="JA154_01090"
     CDS             complement(34781..35260)
                     /locus_tag="JA154_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7994451.1"
                     /translation="MAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQDFNNE
                     LKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKIGKIL
                     LEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYMELKL"
     gene            complement(35333..35773)
                     /locus_tag="JA154_01095"
     CDS             complement(35333..35773)
                     /locus_tag="JA154_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine acetyltransferase"
                     /protein_id="HAT7994452.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            35934..36992
                     /locus_tag="JA154_01100"
     CDS             35934..36992
                     /locus_tag="JA154_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HAT7994453.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGGAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37134..37958
                     /locus_tag="JA154_01105"
     CDS             37134..37958
                     /locus_tag="JA154_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HAT7994454.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38371..39492
                     /locus_tag="JA154_01110"
     CDS             38371..39492
                     /locus_tag="JA154_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994455.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39510..40385
                     /locus_tag="JA154_01115"
     CDS             39510..40385
                     /locus_tag="JA154_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994456.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40409..41101
                     /locus_tag="JA154_01120"
     CDS             40409..41101
                     /locus_tag="JA154_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT7994457.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41109..41933
                     /locus_tag="JA154_01125"
     CDS             41109..41933
                     /locus_tag="JA154_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAT7994458.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            41938..43005
                     /locus_tag="JA154_01130"
     CDS             41938..43005
                     /locus_tag="JA154_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HAT7994459.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43007..43543
                     /locus_tag="JA154_01135"
     CDS             43007..43543
                     /locus_tag="JA154_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAT7994460.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43611..44216
                     /locus_tag="JA154_01140"
     CDS             43611..44216
                     /locus_tag="JA154_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994461.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44228..45142
                     /locus_tag="JA154_01145"
     CDS             44228..45142
                     /locus_tag="JA154_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAT7994462.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45184..46356
                     /locus_tag="JA154_01150"
     CDS             45184..46356
                     /locus_tag="JA154_01150"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087716.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAT7994463.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46403..47215
                     /locus_tag="JA154_01155"
     CDS             46403..47215
                     /locus_tag="JA154_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAT7994464.1"
                     /translation="MFEKIAQSSKFINSKSDIKPKIGLILGSGLGDLANDIEDPVAIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47404..48744
                     /gene="deoA"
                     /locus_tag="JA154_01160"
     CDS             47404..48744
                     /gene="deoA"
                     /locus_tag="JA154_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087718.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAT7994465.1"
                     /translation="MEISIMRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQAS
                     ALLMSIYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIP
                     LVASCGAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSA
                     KVAIADKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEE
                     SFELAKSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVEL
                     CEVLGAYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLF
                     KKSKYKYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDY
                     VNEGEVLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            48957..50036
                     /locus_tag="JA154_01165"
     CDS             48957..50036
                     /locus_tag="JA154_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAT7994466.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
BASE COUNT        20299 a         5065 c         9368 g        15385 t
ORIGIN      
        1 aaatattagt attaaactgt gggagttctt cattaaaata tcaattaata gatatgaata
       61 atgaagaagt tttatgtata ggattggttg aaagaatagg aatagaaggt tctatattaa
      121 aacatgaaaa agcaggtaga gatgataaat acgttgttga acaaccaatg aaggaccaca
      181 aagatgcaat agcattagtt ttagaagctg ttgctcatcc agaatttggt gcagttaaag
      241 aaatgaaaga aatagatgca gtaggacata gagttgtaca tgctggagaa aaatttgcaa
      301 cttcagtagt aataactcca gaagtagaag aagctttaaa agaatgtata gatttagctc
      361 cacttcataa tccagcaaac ataatgggaa tagatgcttg taaagctata cttccagatg
      421 taccaatggt aggtgtattt gatactgctt tccatcaaac aatgcctaaa tcttcatact
      481 tatatggttt acctcatgag ctatatacta aatatggggt aagaagatat ggattccacg
      541 gaacttctca taattatgta tctcagagag cagcagagat attaggaaaa gatataaaag
      601 atttaaaaat agtaacttgt cacttaggaa atggtgcttc tatagctgct gttgatggtg
      661 gaaaatgtgt agatacatct atgggattca ctccattaga aggattaata atgggaacta
      721 gatgtggaga tatagaccca gctatattac cattcttaat gagaaaagaa ggtttagatg
      781 ctgatggatt ggataaatta atgaataaag aatctggagt atatggaatg actggaattt
      841 ctagtgactt cagagatata gaagatgcag ctaaaaatgg agacgaaaga gctcaagcta
      901 cattagaagc gtatgttaaa aaagtacaaa aatatatagg cgcttatgct gctgaaatga
      961 atggattgga tgttgtagta tttactgcag gcgttggtga aaatggaaag gctataagag
     1021 cagatattgc ttctaacatg gagtttttag gaatgaaatt agataaagaa gctaatgatg
     1081 ttagaggaaa agaaacagta atatctactg ctgattctaa agtaaaaatg cttttaatac
     1141 caactaatga agaattaatg atagctagag atacattaag attggtaaaa taattaaatt
     1201 aaacctatgc ctatcaagta aaaatacttg acaaacaaat gctagatttg tataataaat
     1261 ttggtgatat gttgaggtga acttaatgaa agttagcata gaaaaaataa atagaaaaga
     1321 aactgacaaa atagacttga atttttgtga aaaaattgat actattagtt attgtgatga
     1381 gatttacaaa ttagtatcac ctgtaaattt aaaagggaaa gtgtcaaaga ctaataaagg
     1441 tttgtatcta gatatagatg tcaattttac aattgttgac aattgttcaa gatgtcttaa
     1501 agaagtagaa ataccactag agtattctat acaaggtttt ttagtaaaag aagaagatta
     1561 tgatgaagat gagtttgagg aatttgatcc ttttatattc gatggtgaag aaatcgacct
     1621 tattgatata atagaacaga cattagattt taatgtacct cataaagttc tttgtagtga
     1681 aaactgtaaa ggtctttgtc aagtatgcgg ggcaaactta aatgaagaag agtgttcttg
     1741 cagtgaaatt acaaacgatg aggaatacat agatcctcgt tttgctaaat taaaagattt
     1801 atttaattaa attttgacta aggaggtggc gtaaatggca gtaccaaagc gtaaaacgtc
     1861 taaatcaaac acaaaaatga gaagagcagc taactcaaag atggaagcaa caggatttgt
     1921 aagttgccca caatgtcatg agccaaaatt accacataga gtgtgtccag actgtggata
     1981 ttataaaggt aaagaagttg tgtctaagta atagcttcta aaaagcatgt agagtatact
     2041 ctacatgttt tttttgtaca tataaaaaaa taagatgatt tactagagaa aataagtgga
     2101 ttttatgcat attaaaaaat agaaaaataa taatataagt attgaaaatt tttgtgtgtt
     2161 actatatact actattagta gctggtacta attacagcta taaaatttgg agggaacatg
     2221 aaaaagaaaa gtaaagccca aagacaaaaa gagcttatag atatgctaaa gacagatcct
     2281 ttttatactg atgaagaatt atcaagtctg tttgatgtaa gtatacagac cataagatta
     2341 gatagaatga gtctaaacat accagagcta agagaaagag tcaaatcaat agcagaaact
     2401 cagagttcaa aagtaaagac tttaggagta aaggaaatta caggagaaat cattgattta
     2461 tctgtagggc gattaggtat atctatgtta gaagttacac aggacatgat ttactctaag
     2521 actaatactt taaaagatac gtatattttt tctctagcag attcattggc aatggctata
     2581 atagatgcac ctaaggttat tatgagagtt gcaaatgtaa aaagttttaa gttgatagaa
     2641 caacaagata gacttatagc aaaagcagaa gtgtatagaa atatagataa aaagcattat
     2701 gtaaaagttg ttatcaacaa taaagcccaa gagcaaatat ttagaggaaa gtttatcttt
     2761 gaagaattag attaggaggg aaattatgaa aatagtaatt gatggaatgg gtggagataa
     2821 tgcacctaaa tctaatgtag aaggcgcagt aaatgctata aaagaatatc aggttgattt
     2881 aataatcaca ggagataaag atttacttga aaaggaattt tcaaattatg aatttgatag
     2941 aaataaatta gagatagttc atactactga gattatagaa aacgaagata aaccagtaaa
     3001 ggctataaga agtaaaaaag attcatctat ggtagtagca ttaaatcttg taaaagaagg
     3061 aaaggcagat gctataatat cagctggaaa tacaggagcc ttacttgctg gtggattatt
     3121 tgttgttggt agaataaaag gaatcgatag accttgctta tgttcagcaa taccaaatgt
     3181 aaagcgagga atgacactta tagcagactg tggcgcaaat gcagattgta aacctaagaa
     3241 tctagttgaa tttgcagcta tgagtaatat ttattcaaga aaagttttag gtcttgaaaa
     3301 tcctaaggta gcattagcaa atgttggact agaagaaggc aaaggcaatg atttagtcaa
     3361 aaggtcatat gaagaaatta aaaaattaga tttaaatttt atagggaatg tagaagctag
     3421 agaagttata aatgcataca cagatataat catatgtgat ggatttacag gtaatatatt
     3481 acttaaatct gctgaaggtg tagcactttc agttatgagt cttataaagg aaacttttat
     3541 ggccagtaca aaaagtaaaa taggagcatt acttataaag gatgatttaa gaaaattaaa
     3601 aagttttata gattattctg agtatggggg agctccactt ttaggtctaa atggaggagt
     3661 tataaaagct catggtagtt ctgatgctaa agctataaaa aatgctataa atcaaggaat
     3721 aaaattttca aagggaaaag tcgttgaaga tattaaccaa tttataagca aatacaatga
     3781 agaaaataaa aataacgaag atgaataaac tcgttttgga ggtttaatat ataatgaata
     3841 caaaggctgg aatattagga gtaggaagtt atctacctga gcaatcatat gataattttc
     3901 acttcgaaaa aataatggat acatcagatg agtggataag tactagaact ggtattaagg
     3961 aaagaagatt tgccaaagag tctgaagcaa cttctgactt agcatctaaa gctgccttaa
     4021 aagctataga atgtgcaaag ttaaacgtag aggatataga gctgattata ttagctacaa
     4081 ttactcctga tatgtctttg ccttcaactg catgtatagt acaagatgca ataggagcag
     4141 taaatgctac tgcatttgat atatcagcag cttgttctgg atttgtatat ggagtaacta
     4201 ttgcaaagca atttgtggaa acaggttgct ataaaaatgt gcttgtaata ggagcagaaa
     4261 cttgttctaa gtttttaaat tatgatgata gaacaacagc agtattgttt ggagatggag
     4321 ctggagcagc tgttattggc cctgtaaatg agggtggaat actgtctaca catatgggct
     4381 ctgatggtaa aggaaaagat tgtttaaaag tacctgctgg aggttcaaga cttaaggcaa
     4441 gtaaagagac tgtagaagct aatctacata caatagaaat ggcaggtagt gatgtgttta
     4501 aatttgcagt tagaaagatg gcagaaactt ctttaagagc tcttgaaaaa gcaaatctaa
     4561 atacaactga tatagactac ttagtgccac atcaagctaa tataagaatc atacaagctt
     4621 cttctaagag attagagctt gatatgaaaa aagtttatgt aaatatagac aaatatggaa
     4681 atatgtctgc tgcatctata ccagtagcat tagatgaagc ttatagagaa ggtaaaatta
     4741 aaaagggtga taatgtagtt ttagttggct ttggtggagg tctaacttgg ggggcttcag
     4801 ttgtcaagtg gactttatag tataaaatat ctaaaatgat ttaattaaca aaattaaact
     4861 tatacacaaa ataaagttga ttaataataa aaagtttact tttatgattt caagtgaagg
     4921 agaattgtat gaataaaatt tgcaaaatat taaatataaa atacccagtt atccaagggg
     4981 gaatggcatg ggtagctact gcatcattag caagtgctgt atctaatgca ggaggacttg
     5041 gcataatagc agcaggaaac gcaccaaaag aagctataaa gaaagaaatt gttgagtgta
     5101 aaaaattaac agataaacct tttggagtaa atgtaatgct tatgtcgcca tttgttgatg
     5161 atataattga tttgattata gaagaaaaag ttcaagttat tactactggt gctggaaatc
     5221 ctgcaaagta tatggataga ttaaaggaag ctggaacaaa ggttattcct gtagtaccta
     5281 caatagcttt ggcacaaaga atggaaaagc taggagctac agcagtaata gcagaaggta
     5341 ctgaaggtgg aggacatata ggagaactta ctactatggt cttagttcca caagttgctg
     5401 atgctgtaaa catacctgta atagctgctg gaggaattgt agatggtaga ggaattgcag
     5461 catcatttgc attaggtgcc agtgcagttc aagtaggaac tagatttatt tgcagtgaag
     5521 agtgttctgt ccattcaaac tataaaaact tagtactaaa agcaaaagat agagatgcaa
     5581 ttgtaacagg aagaagtact ggtcatccag taagaacatt aaaaaataaa ctatcaaaag
     5641 aatttttaaa gatggaacaa aatggagcta ctcctgaaga actggataaa aaaggtacag
     5701 gagctttaag atttgcaaca gtagatggag acatagaaaa aggttcattt atggcaggtc
     5761 aaagtgctgc tatggtaaaa gaaataacac cttgtaagga aattatagag gctatggtaa
     5821 atcaagcaag agagattatg ccagcaatag aactgtaaat caggaggagc aagatgggaa
     5881 aagttgcact agtattccct ggtcaaggag ctcaatatgt aggtatggcc aaggatttat
     5941 atgaaaataa tactgttgct aaaagtgtaa tagatgaggc tagtgatgcc ttaaatatgg
     6001 atttaaaaaa ccttatgttt aatggaaatg aagaagaact ttctaaaact gaaaacactc
     6061 aacctgctat ggttacacat agtgtagctg tattaaaagc agtacaagcc caagtagatt
     6121 taaaatatag tgcatgttta ggtttatctt taggagagta tagtgcattg gttgcagcag
     6181 atgcaattga ctttaaagat gctgtatgct tggtcaaaaa aagaggtaag tttatgcagg
     6241 atacagttcc aacaggtgtt ggagctatgg cagcaatttt aggtctagat agaagtatat
     6301 tagaaaatgt agttaaagat atacaaggag gtattgtgga agtagcaaat tataattctc
     6361 ctggtcaaat agttatatct ggtgaaaatg aaaagataga agaagcaatg gtgaaatgca
     6421 aagaagcagg agcaaaaaga gctgttaaat taaatgtaag tggacctttt cactcatcaa
     6481 tgctaagaga agctggtgta aaattggcca gtgaattaga aaaagttaac ataactaaac
     6541 ctaaagttga tgtggtggca aatgtaaatg cagattacta taaaaacgaa ggtaaagacc
     6601 tgcttataaa acaagtaagt tcatctgttc tttgggaaga ttctatagaa agacttttaa
     6661 atgatggata tgatactttt atagaaatgg gacctggaaa aactttaaaa gcatttatta
     6721 agaaaatagc ttccaataaa aaagcaaatg tcaatattta taatatagat ggtatagatt
     6781 ctcttaatga atttgtacaa aattatagaa atggagagat ataatgataa atcttacagg
     6841 acaagtagca gttgttactg gtggctctag gggtatagga aaagaaatag caaaaaaact
     6901 agcatctttt ggagctgatg tagtaatcaa ttatacttct aaagaagatg aagcactaaa
     6961 aactaaaaat gaaatagaaa gtatgggggt aaagtgtacc tctataaaat gtgatgtgtc
     7021 taaatttgat gaagtgaatc aaatgataga ttctgttgta agcgaatttg gaaaaattga
     7081 tatattggtt aataatgcag gcataactaa agatggtctg cttatgagaa tgaaagaaga
     7141 agattttgat agagttatag atataaactt aaaaggtgtc tttaattgta caaaagcagt
     7201 tactaaacct atgatgaaaa agaagtatgg aagaataata aatatgactt cagtagttgg
     7261 aattatgggt aatgcagggc aaactaatta ttgtgcatca aaagcaggtg taattggatt
     7321 tacaaaagct tctgcaagag agttagcatc aagaaacata aatataaatg cagtagcacc
     7381 tggatttata gaaacagata tgactaaagt actaagtgat gatgtaaaag aatcaacact
     7441 agcaaacata ccaaagaaat cttatggtaa accagaagat gtagccaatg ccgtagcatt
     7501 tttagttagt gacatgtcaa gttatataac aggacaagta ataaatgtag atggtggaat
     7561 ggtaatgcaa taataattta aaaagtagag aaatctatta aaataaaaaa ttttaggagg
     7621 tacacaatgt ttaataaagt tgtagaaata ataatagagc aattaggagt agaagataaa
     7681 gaaataacta tggaaacatc attaatgaag gatttagaag cagattcatt agatgcagta
     7741 gaaataataa tggcacttga agatgaattt ggtatagaaa taccagatac agaagcagaa
     7801 aacttcaaat ctataggaga tatagttaag tatatagaag ctaataaata gtcgagcttt
     7861 aaaaaagtgg aggtatccga tgaataaaag agtagtaata acaggacttg gatgtgttac
     7921 gccgttggga acaggaaaag aagagttctg gagtaatata aaatctggag tatctggtat
     7981 agataaaata actaattttg atgctagcac atatcaaact cagatagcag gagaagttaa
     8041 gaattttcat ccagaagaat atataagtaa aaaagaattg aagagattag ataaatttgc
     8101 tcaatttgca atagtatctg ctaaattagc agttgaagat gcaaatcttg atttagataa
     8161 agttgataga gaacgttttg gtgttataat aggttctgga ataggtggag ttgaagctat
     8221 agaaactcaa cataaaattc ttttggaaaa aggaaataaa agagtaagtt cactttttgt
     8281 acctatgatg ataggaaata tggcagctgg tcaagtttca atatttttag gagcaaaagg
     8341 gcctaacact aatgtatgta cagcttgtgc atcaggaact cattcaatag gtgatgcttt
     8401 taaggtaatt caaagaggtg atgcagacat catggtagca gggggttctg aagctgctgt
     8461 aacaggtctt gcttttgctg gattctgtaa catgaaggct atgtcaacta gaaatgatga
     8521 ccctaaaact gcatcaagac catttgataa agacagagat ggttttgtaa tgggagaagg
     8581 agcaggtatt gttatcttag aagacttaga acatgcttta gcaagaggcg ctaaaatata
     8641 tgcagaagta gttggatatg gtttaactgc tgatgcatac catatgacaa ctccagctga
     8701 aaatggagag ggagcagcta ggtctatgaa tatggctttg aaagatggaa atgttccatt
     8761 agaagaagta gattatataa atgctcatgg aacttcaact tattacaatg atttatatga
     8821 aactatggct ataaaaactg tatttggaga aaaggcatat gacttatgtg tgtcatcaac
     8881 aaaatctatg actggtcact tattaggagc atcaggtgct atagaggctg ttgtttgtgc
     8941 tatgagtata gaagatagtt ttgtgcctcc aactataaac atacaagaag ttggtgaaga
     9001 tttggattta gattatgttc caaatcaagg taaagaaaaa aatattagat atgcactatc
     9061 aaattcttta ggatttggtg ggcataatgc tacaatagta ctaaaaaaat atgtataaat
     9121 aaatattaaa tataaaacta aaaaacaagc acttgtttta actgtataaa tttatattag
     9181 cttaaattta aagtctccta aattatttta ggagactttt ttattaagat ttaagtctag
     9241 catatgttgt aaatcatttt gatatattgt attattaatt attaagaggt tatttatata
     9301 tgtaagtttt cattctacaa tatacaaaag gaggactaaa attttgaatt ttagactacc
     9361 aaagaaaagg ttacccaaaa agataatttt tatatgtata atacttatta gtattatttt
     9421 gtttataaag catgaccaaa atggattgaa agaaagagaa attataaata agggaaaata
     9481 tatgtctgaa agccttgtgg cagataagga gtatgatgaa gcaaaaaaag tggttaagac
     9541 ttctttcgaa tctattccag aaaaaaaata caataaagta tataatgaag tttgggatat
     9601 gttaaagact atttcatatg ttccagatgg aacgaagata gtcattgatt ctttagaaaa
     9661 gatgagagaa tcagatgtga aattatctga tgaatgtaga tttaatatag aaaaaagatt
     9721 agcatcagtg tatataatgg ataataatta ttctaaggca gtagatttaa ctgtaaagtc
     9781 tataaagttg gctgagaaat tggggaataa ttatgaaaaa gcaagactag atgttgattt
     9841 ggcgagcata tttttaaaga taggtggcta tgaaacagga gagaaattaa taaaggattc
     9901 ttttaaaata gatattgatg aaggtgaaaa aaatggaatg gttagattat atgctattat
     9961 aaatctagca gaaatatatg tagaagaggg cgaatatgat aaagctattg aaataagtag
    10021 caggataaaa gattataaaa agtttgtaaa tacggataat tacaatgatt ttgatataat
    10081 ggcctctata atgcagtcaa atgcgtatgt gggcaaaaat aatatacaaa aggcaaagac
    10141 ttttttagaa aaagctgatt atcttataaa atcagataga acagtttata tattagataa
    10201 agatatgtac tattatatgg caatgggaaa cttagaatat aaaagcgaaa attataattc
    10261 tgcaatagat aattataaaa aaagtttaga gatatctact aaaagaaagt taacccagga
    10321 gaaaataaaa aatttaaata agatattaaa tatatatgaa aaacaaggaa atatagaatc
    10381 tttaaatgag tatcaaaaga tgttaataaa tgaatataaa gagaacaaaa gtataagaga
    10441 ttcagagact agtttctata taattgataa agtatcaaac gaaagtaagc tatttgaaaa
    10501 aactaaaaaa gaaataaaat attataaaat attatttaca gtaattttaa ttgctatatt
    10561 agggtctact tttttataca accgattgaa gtattttaaa gcacaaaatt tacatgatgg
    10621 tcttacaaat gtttataata gaaaaagttt tgatattatg tatgaaaagt acagagagaa
    10681 agatgataat tttgcacttg tgatgataga tattgataat ttcaagctca taaatgataa
    10741 ttatggacat aaatttggtg acactgttat aaagggaata acaaatacca tttgtgttat
    10801 gttagaacaa gatgataaag tctttaggta cggaggagaa gaattttctg tattaataag
    10861 aaataaatct ggtgatgaag tagcagatat aattgacagt ataagggttg caattgcaaa
    10921 taaaaagtgg aatgatgatg tgactgtgac tataagtgca ggtgtagctc atgtttcaga
    10981 tagcaaagat gtactagaag aagctgataa aaatttatat aaagcaaaac aacttggtag
    11041 aaataaagta gtatataagt aattatatac tatataaata agaggtataa atatgaaaca
    11101 atcaatagct atagtaactg ataaaataac taaactatca tctttcttag aagaaaatat
    11161 acgtttggtt ttaggtaatt atattgatat aaaccactat tatctggaag ttttaaaaga
    11221 gaatgataaa atacaaggtg attttgtact agtaatgaat gatgatagat taaacagtat
    11281 gagaaagcat ttagtacaag atagtaagat tatagtgata aggagaacac ttaaagaaaa
    11341 tgaaatatat ggattatttt caataccaaa tggaactgaa gtattggttg taaatgatac
    11401 aaaagaaact accttagaaa cgataagctt attttacaaa ataggagtaa aaaatttaaa
    11461 gtttactcca tatattgaag gaaaaattta taaaaatata gacatagcta taacgcctgg
    11521 agtaaaggga aaagtaccaa gttatataaa taaagtaatc gaccttggaa atagatatat
    11581 tgatatatct acctttatag aaataataaa tggtcttaga atagatgtta aagaaatacg
    11641 taaaaattta atggcatatt ctgagagttt aataagttta gataatggag ttaaagataa
    11701 ttataggcaa ctgtttttaa aaatagaaga acaagatgtt atattgaatc tatcaaaaga
    11761 tggaataata tttacttcag cagatggtat aataaacaca tttaataaag aagctttaaa
    11821 aattttaggt ataaatgaaa atccacaagg caaaaacata gaagatatca tttctgaaga
    11881 tttaaatatt ttacttgaag acaaagaaat aattgatgaa gtggtattta caaataaaaa
    11941 atatataaat gtaaataaaa aaagtatttt ttctatgggg aataaagctg ggatatatta
    12001 taacttacaa gaaataacgt atataaaaaa attagaacaa aatcttacta ataaattaag
    12061 agaacaaggt cagatagcaa gatatacatt taaagatata aaaactaaaa ataaaaatat
    12121 ggtaaagtgt gtagaattgg ctaaaaaaat atctaaatca gaactttctg tattaataat
    12181 aggagaaagt ggtactggta aagaattatt atcacaatca attcataatg cttccaatag
    12241 aagtaatcaa ccgtttatag cagttaattg tgcagcagtt ccagatagtc tattagaaag
    12301 tcaactgttt ggatatgaaa aaggctcttt tacaggagct ttaagagaag gaaaaaaggg
    12361 tctatttgaa cttgctaata atggaactat atttctggat gaaattggag atatgcctcc
    12421 attgctacag acaaagttac taagggtatt acaagaaaag caggttatgc ctataggttc
    12481 ccataatgtt ataaacatag atgttagggt catagcagca acaaacaaaa acttactaaa
    12541 gatgattaga gaaggaaaat ttagagagga cctgtattat agactaaatg tattgccaat
    12601 agatgtacca tcattgagga atagaaaaga agatataata acacttatga atttcttttt
    12661 gaaaaacaat ataagacttt cgccagaagt tataactatt ttagaaaaat ataattggcc
    12721 aggaaatata agagagttac aaaatgttgc ttcttatgta agtctaatgt gtgatagtat
    12781 ggtttttaga gatgacttac caccatatat aagggtagaa tgtgctaaca attatgaaga
    12841 tggatttggt tatacaagaa aagaatttga ggaaatactg aggattctat atgaaagaag
    12901 agagtcatcc tctggaattg gaagagggtt tataatcgaa aagcttatac aggaaggtat
    12961 tgagatttct gaaagcaaag ttagaaaggt gctatcttat ttaagtgaat gtggatacat
    13021 atcatctaaa tctggtagag gaggaagtca aataacctca gaggggataa aactttataa
    13081 taatataaat taagctaagt atttgatatt gaaatcacat attttgaatt tagatattta
    13141 aataataata aacttttata tttatagaga ttaatttata ttatgtcaga aagaatattg
    13201 taatttataa ttaaatatta ataggttttt aaaataggat tagataggta ataggttaag
    13261 ttagaataac ccattaccta tttttatttg taagaaaagt gtacatagaa ttagaagtaa
    13321 aaaatacaac taataaaact attgaaaata ctagataaat aataaataaa aataattata
    13381 gattttggca tacatattgc taattataga gataagacga caaaataatt gggaggttta
    13441 atgatgaaat acaaaacaac agggacttgt gctacagaaa ttgaatttga agtaaaagaa
    13501 aataaagtta caaatgtaaa ctttataggt ggatgtgatg gaaatttaaa agggctaaaa
    13561 gtattagttg aaggcatgaa tatagaagat gtaatacaaa agttaaaagg aatagagtgt
    13621 aaaacaaagc caacatcttg cccagaccaa ctgtctctag cattggaaaa ttatatgaat
    13681 ataaagtaat acataaaaca atcgattagt taggagttta attatgaaac caataatagg
    13741 gattttagga aatttaataa taatggaaaa tggtatgttt ccaggtcttg aaaggtcata
    13801 tgtaaataat gattatataa atgcagtgct aaaaggagga ggtagtccag taataattcc
    13861 tgtaaataca gataaagaag ttataaaaaa acagattgaa atggttgatg gagtcttaat
    13921 atctggggga tgggatatca atcctcaact gtatggagaa gaaacaagag aagaaacgac
    13981 atttatatat ccagaagtgg atgaatttga tttaattgca ataagtatag cattagaact
    14041 taaaaagcct atattaggtg tatgtagggg actacaaatt ttaaatgtaa gtttaggagg
    14101 aactttatat caagataata atcttataga aggaagttat ataaaacata cacaatcttc
    14161 taaaagacat gtagctactc ataaagttga tgttaaagaa ggaagtattt tagagggaat
    14221 tttaggaaaa caattactta caaatagtta tcatcatcaa agtgtcaatc agcttggaaa
    14281 aggattaaaa gccatagcat actcaaaaga tggtataata gaagctattg agaaagagga
    14341 tgaaaatttt gtagtaggag tacagtggca tccagaaatg atggtagatt actgtgataa
    14401 aatggaaaaa ctatttaaat attttataaa tatttgttca aatttataat ataccttggg
    14461 gggattgaac atgaacgaaa agaacaaaat gggattaata agtattatac ttttaggtat
    14521 taatgcagta gtaggagctg gagtttttct attaccagga gatgcaatga aatcatttgg
    14581 tgtagcaagt atatttgtat atatatttga tatgttgtta gttttatcta tggcattctg
    14641 ttttgctgag gttgcaggta aattcaataa aaatggtgct gcctatgtat atacaaaaga
    14701 agcttttgga gatttttgtg gttttgaagt aggtcttatg aaatgggtaa taggatgtat
    14761 ttcttgggga gctttaatag taggttttcc tacatcatta tcagcagtat gggctccagc
    14821 aggtgaacca catatacaaa aaattataat tgtagtcatg atagttgggc ttacaattat
    14881 aaacttatta ggtgtatctc tatcaaaaat tgtacaaaat gttataactg taggtaaact
    14941 aattccatta atactattta taggaatagg tatatttttt ataaaaggag ttaattttac
    15001 ttcttcaaca atggtacctc caggagctgg agcaacagag tttggagcag cagcactttt
    15061 aatgttttat tcatttacag gatttgaatc tatagcagtt gcagcagaag atatggagaa
    15121 tccacaaaaa aatataccaa tagcaattat aagtgtaata gtaattgcat caataatata
    15181 tatattaaat caagttgtat gtgttggtat actaggagat tcgttatcta gtacatctac
    15241 accagtggca gatgctgcaa gaatatgctt tggaaatatg ggagctggat tggttacctt
    15301 tggaaccttg gtttctgttg gtggaatttg tatgtgtggt gcttttgtaa atccaagaag
    15361 ctgtgtagct ttagctgatg ataaaatgtt accgagaata tttgcaagaa aagataaaaa
    15421 aggtacacca tatgtagcga taatagctac tatgctaatc actataccaa tagctctatc
    15481 tggaagtttt gctgaattag cagcaataag tgcagttgct agattcatac aatacatacc
    15541 aacatcttta tccgtattag tgtttagaaa gaaaagacca gaattagtag gtacttttaa
    15601 aactcctttt ggagcagtga ttcctctaat tgctgtatgt gttggaacat ggttattatt
    15661 ccaagcatct atgcatcaat taattatggg attaggagct ttggcaattg gagtaccatt
    15721 atactttata atgaagtctt ataatagaaa agtctatgat gaaaatttaa agaaaatagt
    15781 ttaattatgt tataacaatt tattgagtta aaattagaca ttggcataaa attaaataca
    15841 aataagtatc tacaaggcta tattaaaata ttattaattt tttattttaa tatagcctta
    15901 tattgttatc aataaatgaa aaatattgta acaatagaag aacaatagta ttatgtttat
    15961 agacaaaaaa cttattagga ggtaaattta tgccaacaat aacattaaaa aatggtgtag
    16021 acgtacttat tagagaaggt gtaagagaag atgctcaaag cattatagat ttttataatg
    16081 aagtgggtgg agaaactcat tttctgtcat ttggaaagga tgaatataaa atatctttgg
    16141 aggaacaaga aaatgctata gaatcagcaa aagcatccga taattctgtt aaactgattg
    16201 catttataga tggtgaaata gttgggattg caacgattga ttctaatcaa aaagcaaaag
    16261 gaaagcatgt tggagtatta ggaattgttg taaaagaaaa atattgggga attggattgg
    16321 gtaaaaggct tatgttagac cttatagaat ggtgtaaatc aaatggaata accaagaaga
    16381 taactcttgt cactaatgaa gaaaattata atgctatagg attatacaaa aaagttggat
    16441 ttgaagtaga aagcatacta aagaaagaat gttattataa tggtgtttat actgatttaa
    16501 taggaatgtc acttttattg ggaatataat tagtgtacat tttatctcga aaaaagtata
    16561 ttaagtatgg tataattaaa aaagaatata acttaatata attttggagg acgaattatg
    16621 aaaaatctgt gtaaaatatc agatgattat ttaaacagca agcttaagta tttaaagtta
    16681 ttatcaaaac aatatccaag tatatcaaaa gcaagtacag aaataataaa tttagaggct
    16741 atattaaatc ttccaaaggg aacagagcat tttataacag atgtacatgg tgaatatgaa
    16801 ccatttgtac atgtattaaa aaatggttct ggtgtaataa agagaaaaat agaagaattg
    16861 ttttcaaata caataagaga cagcgaaaaa aaaatgttag caactcttgt atattatcct
    16921 gaacaaaagt tagacctaat aattaagcaa gaggaaaata tagatgattt ttatagaata
    16981 aatatttata gacttataga actttgtaaa tatgcttcta gcaaatatac taggtctaaa
    17041 gttagaaaac tgttaccaga aaattttaaa tacataatag aagaactttt gcatgagcat
    17101 gttaaaagtg aacataaaga agagtattat aaaagtatag ttgaaacaat agttgacata
    17161 ggaatagcta aggagtttat aattgctatt tctacagtta tacaaaaatt ggttgtagat
    17221 agacttcatg taataggaga tatttatgat agaggtccaa gaccagatat aattgtcgat
    17281 aagcttattg aacatcattg tgtagatatt caatggggaa atcatgatat attgtggatg
    17341 ggtgcagcct caggtgaaaa aacttgtata gcaaatgcac tgaggatatc agctagatat
    17401 gcgaatttag acattgtaga agacatatat ggaataaatc tattgccttt agccactttt
    17461 gctatagaaa tgtataaaga tgacccttgt aaagaattta ttccaaaggt taatgaccaa
    17521 agtgtaacaa caacagaaaa gtctttaatg gcaaaaatgc acaaagcaat aagtataatt
    17581 caatttaagc ttgaaggtga ggttatcaga cgaagacctg aatttgaaat ggaacataga
    17641 ctcttgctta atatgataaa ctatgatgaa ggtacgatta atttaaaagg taaaacatat
    17701 aaattaaaag atacttattt acctacaata gacaaaaaag acccatataa attaactatg
    17761 gaagaaagaa atgttattga taaattagta tcttcattta gaggtagtga aaaattacaa
    17821 aaacatgttt catttttgtt ttctaaagga agtatatatt taaaggctaa ctcaaattta
    17881 ctgattcatg gatgtgtacc gttaaatgaa gatggaagct ttatgtctat gaatataatg
    17941 ggaaaagagt ataaaggtaa ggctcttatg gataagatgg agtctctagc aagagaagga
    18001 tttttcttta aagataaggc agaagaaaaa ctgtatggca tggatattat gtggtattta
    18061 tggactggga aatgttcatc attatttggt aaagatgata tgactacatt tgaaagatac
    18121 tttatagctg aaaaagagac tcataaagaa aataagaacc catattttaa acttagagaa
    18181 aatgagatgg cttgtaaaag attatttgaa gaatttgatt tagaacttga tgaatctcat
    18241 ataataaatg gtcatgttcc tgtggagagt aaaaatggag agagcccaat aaaagctaat
    18301 ggtaaaattc tcgttataga tggtggtttt tctagggctt accaaaaaac tactggtata
    18361 gctggataca ctttgatata caattctaga actttgcaat tagtatcgca tgaaccattt
    18421 aattctgctg aagaagccat tgcaaatgaa agtgatatct tatcgacaac tgttgtagta
    18481 gagcataagg caaagagaaa aatggtgaga gatactgatg aaggagtaaa gatacaagag
    18541 gaaatagaag atttaaaact acttttaatg gcttataaga aagggctaat aaaagaaatg
    18601 tagatgttat agaggcactt catgattgaa gtgtcttttt gttaatactt attaaatata
    18661 ttataaagat taataatagt agtttatatt atacttacta agtgaatcca agataaatgg
    18721 gttcactttt tttatttata aacattatta aaaaaatata gatgttttgg ttatattcct
    18781 tgtcctttat aaatagaatt atataaacaa aggaggagga caagagatga ataaactttc
    18841 tgatgaaata ataaactctc tttctacaac tataagagaa aaggtagaaa aagtctcaaa
    18901 caataatctg aacatagaag aaattaggtt acgctcacaa aaaccattga tactaaatgc
    18961 aaactctaaa gattattttt acaatcaaaa aactatgaca cttgatttaa atcaacaaaa
    19021 ctcatatgta gtaacaagag aagatgtaga gcaaactttt caaataatat gtaaatattc
    19081 aatacattca tttatggatg acataaaaaa aggattcata actttgagag gaggtcatag
    19141 agtagggtta gttggaaaag caatagtaga agatggacaa gtcaaaaata taaagcatat
    19201 atcatcttta aatataaggg tatcaaggga aattattgga tgctcggata agattttaag
    19261 tcatatcata aagggaaaaa atcagattaa taatactttg ataatatcac cacctcaatg
    19321 tggaaaaaca actttaataa gagatatagt aagaaattta agtaatggta atgaagatta
    19381 tggatttaaa ggtttaaaag tggctttagt agatgagcgt aatgagatag caggagctta
    19441 tctaggtgtt ccacaaatgg atgtgggaat aaggactgat ataatagaaa cttgcccaaa
    19501 agacttgggt attacaatgc ttttaaggtc tatgtcacca aatgttattg tgacagatga
    19561 aattggaagt gaaaaagaaa taaaggcatt atacacagca ttaaatggag gtataggatt
    19621 aataactact gtgcatggag attcaataga ggatatacaa aatagaaaag aattaaatag
    19681 acttctggat aaagagttat ttaaaaaggt tataattctt tctgcaaaaa gaggagcagg
    19741 aacaattgaa aaaatttatg atttagaaga aaagagatgg tattttgcaa attaaaataa
    19801 ttattatagc tttcttgata ggaagtagtt atttgatagg tgaacaaata tacaaaactt
    19861 atactcgtag acataaacaa ttaaacgatt taataagagt tttagaaatt ttgaggatgg
    19921 atttatcttt tggactttat acattagaag aaatctttaa taggataggt ggaaataaag
    19981 agttttgttt ttggaaattt ttctatcaaa tatcagaagg acttcacaat gaacaaagta
    20041 aaactttgga aataataata tcagaaaata ttgatgtctt aagtaaagaa acttatctag
    20101 gcaataaaga aattgaggaa ctgaaaaatc taatacttac tttagggaaa agcgatatag
    20161 agtcacaaca aagaatgata gatttatcta tagaaaattt gaaaaaacaa acctatgaga
    20221 caaaggaaga tataaacaaa aaaggcgtat tgtataaaaa attagtgaca ttcataggta
    20281 taggtatatg tattatattg atttaatatg gggaggttta ttatggaaat atcattgata
    20341 ttaaaggttg caggtgtcgg tattctaata tcagtattga atatgatatt ggaaaaaaca
    20401 gatagaaaag attgggcagg tcttactaca ttagcaggtg taataatcgt attgggtatg
    20461 gtaataacag aaataagtga cttgtttaat actgtaagaa caatgtttca actttactaa
    20521 agaggaggga gtaatttgga aataatgcaa ttaataggaa ttgcaatcat ttcaactaca
    20581 ctctgtttag taattaaaaa agatagacca gagatagcaa atttcatagc tataataact
    20641 ggagtaataa tactattgtc agttatgttt aagctaaatt ttatagtaga tagtatacaa
    20701 gatttggcta acaaggctaa catcccgact atgtatatat cattaataat aaaattaatt
    20761 gggatagcat acttaatgga atttgcgata caactttgta aagattgtgg ggaaggaaac
    20821 attgcttcaa aactagaatt tggaggtaaa ataatagtta tgtcaatgtc tttccctata
    20881 cttttgtcca ttgtagaaat ggtggtgaat ataattccat agatgtgtat cttggaatga
    20941 atataagtat attagagagc ttttgttgta ttgaggttta tagagtgaag agaggcatgg
    21001 ttgcttatga aaaaaagatt tttaccaatg attataggtt ttttatttac tttctttttt
    21061 gtaaatatct ttgctattat gatatttgct aatgaagtac caagtagtga agataaagaa
    21121 tatggtgaaa caaaaaatag catagataag tatatagatg gtcaattaga caagctagat
    21181 ataaatgaga ttcaagacta tataaataaa gaaatagtta ttaatgatgt taatttaaaa
    21241 tcatttgtaa aagatttaat tagtggagaa aaaaatatct tagatttatt caataaagat
    21301 ggattgaaga tattgatgtt tgatgaattt aaagcaagtt taaaggtagt agcagtaatt
    21361 ttggtactgg ctctgttatc atctatttta aagagtctgg aaaactcttt ttcatcaggt
    21421 gcagtaagtc aaatagcaac atatataata tttatcacaa tggtatcact tacacttatt
    21481 ggttttaaag atgtattaca gatttgctat gatgcaatag accatactgt aggtcttatg
    21541 caagttataa tgccaatatt aataacattt ttacttttaa ttggattccc aataacgtcg
    21601 actactttga atcctatctt cataggagga gtaacattta taaatgtatt ttttaaaaat
    21661 tttctatttg tatctattac tgttgcattt ggaattttaa taataaacaa tttatcaaaa
    21721 aatataaggc taaagagatt tttctccttt gtaaaacaaa taaattatgt ttctattgga
    21781 gctatgttta ctgtttactt aggtcttgta tccatacaag gattgtatgt aaccagtttt
    21841 gataaattca gtgtaaagac tgctaaattt gctataggta attttattcc tgttgtaggt
    21901 ggatttgtat ctgactctgt ggatatttta ttatcttcat ctcagcttat aaaaaatata
    21961 tttggaggaa tagggcttat cttacttgtt ggaatttgct tacttccagt aataaaaatt
    22021 ttatcagtaa tagtagtgta caaattagca gcaataattg tagagcctgt tggagaggat
    22081 ggaatatcta attttttaaa tgaagtggca aatttaatga taataatgct agcatcagta
    22141 attgctataa cagtaatgtt ctttgtaact gtagctatct tgacctcgat aagtgtcgta
    22201 agtcaagggt aatttatata tcagtgaaaa ataattacat ttaatctaat gtttttagag
    22261 aggaggatgc tatgttagag ggcataaagg catggatagt tagtgttttg ataggagcat
    22321 ttatagtaaa tatagtagat atgattttac caagttctaa gataaaacct tatgtcaacc
    22381 tagttttaaa ctttatgttt gtatttattg taataactcc agtagtagga tttttttcaa
    22441 aagatatgag tttagaagat agaatattaa aatctatggg aaactacaac aagcagtatg
    22501 tagatagtac aaatgctttg gcaaaagaga ctggaaataa tagtctatcc aagggatatg
    22561 aggatgggtt aaaagaagta cttaaattaa aacttgatga atatggttat gatttagaag
    22621 atatagagct taatggggca aatataaata atataaaaat aaaagaaaag aataatagtg
    22681 caaaaagtaa taacagttcc aatgaagaaa ataaaaataa tagtactaaa agagaggaag
    22741 aaaacataaa ctctaatgac aaagaaaatt ctaaacaagt cttcaaaaaa ggtacagagt
    22801 atgggttgaa cctaaatgaa gaaaaattaa aaaacgactt aataaaggtg ctggatgttt
    22861 ctatagaaga tatacaaatt gataaatagg agttgggaga atgttgaaaa acttaaatga
    22921 caaagataag agaaaggtat attcactaat aactatagca ggaatatgtg tggtttcatt
    22981 agtattatta tcttgttttc ctagtagcaa aagcgataaa caagttggaa aaacagagac
    23041 taataaaaat gctgaaaagc aagtcactaa agaacaagaa aaagatgatt tagagtcaaa
    23101 attaacagca atactatcta aaattgatgg tgcaggagac gttgatgtaa tggttacatt
    23161 tgaatcaagt gaagaaatac aacctgcttt taattcaaat aatacaacag aaacaacaga
    23221 ggaaaaagat gcacaaggtg gagaaagaac agttacgact tctagtgaaa ataaaacaat
    23281 gataacttca aattcaagtg accctgtagt tataaaaact acagagccaa agataaaggg
    23341 agtgatagtg gtagcaagtg gagcaagtga cccaagtgtt aaagagacac tttatagtgc
    23401 agtacaaaca tcattacaag tagctggtca tcaagtagaa atatattcta aataaataca
    23461 tataaccggg gggataaatg aatgaagttt aattataagg gaagaggatt tgtaataata
    23521 actttaactg caatgttagt ggtagtagga acagtgaatt atcaactaag taagaaatct
    23581 ttattagaaa catcaaaaga gtttaaggca tatgaagaag cacagttgca aaaaaatact
    23641 gatgatagtt atagtagtaa gacagaggat tctagtaatg tagataaaca aggtggtaag
    23701 gaaagtgctg atattgatat agtcgatagc aaagctagta aagtaaaaga aaaggcaact
    23761 gagacaagta aggaaataaa agctcaatta tcatcagaga aaaatatgaa aaaggcatct
    23821 tatattttag atatgaaaat gaatagagaa aaacaaagaa atgagttagt tcaagattta
    23881 aatgaaatga taaataatcc atctacaaca gaagaatcta gaaaagaagc ttctaatatg
    23941 aaattaaata tagttaaaat tcaagaaaaa gaacttcaaa tagaaaacct tttgagtaca
    24001 aaaggatatg aagaagcgtt agtttatata agcgataaca aagttaatgt agttgtaaat
    24061 gaagctaaat tagaaaagaa agatgcagca aaaatatttg atttagttgc agaacaagcc
    24121 aatgtgaagt atgaaaatat taaacttaca aataataata gtaataagta aagtataaac
    24181 ctcagaatta actgaggtta ttttcttgca aagtagactt gtatttggta ttattatact
    24241 tataaaaata gttccttaaa atcaggaggt aagttttaat atggaagata ataaatttgg
    24301 acaagtaaaa atatctaatg atgtaatagc tacaatagct ggtttagcag cattagaagt
    24361 tgaaggcata gaaactacag caacattaac agataagttg ttaaaaaata atggagtaaa
    24421 aatacaaatc gaagaagagg atgttaattt agatgtaatg gttacgataa agtatggaat
    24481 gtcaatacca gatacagctt ttaaagttca agaaaatgta aaaaatactg ttgagacaat
    24541 gacaggactg aaggtttctc aagtaaatat acacattcaa ggaatcagct ttaagaagga
    24601 taaagttgat aaagaagaag caaaagcaac taagaaaaat taatattagc cctctgaaaa
    24661 gtcagagggt tttttgctgg taattgattt tatatggata attaaactta aaggagaatt
    24721 ttgattgatg aaaaaagata gggcacaaaa gagtacaaca agagaatata taatgaaact
    24781 tatataccaa ataaatatta ataaagaaga ttttgaaact ttagaagata aagtagataa
    24841 ttttttaaaa gataattcag aacatattat aaatagatat aaagaacttg cacttcaata
    24901 ctcaaaaaat acaaatttaa agttagaaga tactgaaatt gaagatgtta tagacaaaaa
    24961 atatataaat acagtatgca aagcattgaa agaaaatcat gacaaaatag atgagttaat
    25021 aaataagcat gctaaaaact ggacagtaga tagaatgcca aaggtagatg tttctatact
    25081 tagattgtct gtgtgtgaaa tactttattt agacactcct aataaagtat caataaatga
    25141 agcagtagaa cttgcaaaaa tatattgtga tgataagtct cctaaattta taaatggtat
    25201 attgggaagt gttgttgatg aaattgggaa ataatataat aattggaatt gatactagct
    25261 gctatactac ttctatagca gctatctctt tagataagaa agttatattc aatgaaaaaa
    25321 taatgttaga agtaagggat aattcaaaag gtttaaggca aagtgaagct gtatttcaac
    25381 atatcaataa tttagggatt cttagtgata ggataaaatc ttttaaagat aagtttaatg
    25441 tagaaggagt ttgctcatca aaaaaaccga gacctgttga aaactcatat atgcctgtat
    25501 tcaatgtagg tcataatttt ggaaaattat tgtctagtat atatggttgt agattttatg
    25561 agactactca tcaagagaat catattgaag caagtttatt aaatagtaaa ttaaaaaata
    25621 ataataaatt tatttctgtt catatgtctg gtggtactac agagatactt ttaacaagca
    25681 aacaagatag tcatcacaat gtatgtgata ctaatttagg taaaattgcc aaaataagta
    25741 ttaaaaaaga tgataaaagt aagttataca ataattttgg atataatata gatataattg
    25801 gaggaagtaa agatattagc tttggtcaac ttatagatag agtaggaatt aagcttggat
    25861 ataaatttcc ttcaggaaag tatttggatg aaaatgcttt aaattgtaat cttaaaatag
    25921 aaagtggtct gaaaacttct gtaagagatg ggtatatgaa tttatcagga ttagaaaatc
    25981 aggtaaataa gattataaat gataatggag ataatactaa tcaaaaagaa tatatatcta
    26041 aattagtatt agattcagta gttagaaata tgtttaaatc tctagtgtat ttatgtgaaa
    26101 cttataatgt aaatgaagta gtatttgctg gtggagtttc tgcaagtaag tatattctta
    26161 gagaattaag catgaagtta agaaaaaagc atatagaagc ttattttaca gaaccacaat
    26221 attcaactga taatgctgtc gggtgtgcta taataggatt gaataacttt ttaggagaaa
    26281 gagtatgaaa ctaagagctt tagatataag tgaagcaaac tcatatatta aaagaatttt
    26341 aataaacgac ccaatacttt ctaatcttaa agttaaagga gaaatttcta attttaaggt
    26401 tcatagtagt gggaatgtat atttatcact taaagatgaa acatcaaagt taaactgtgt
    26461 aatttttaaa agtaatttta atcgcaattt aaaattagat aatggtgtaa aaattatagc
    26521 taatggatat atatcagtct atgaaagaga tggtgcgtat caattataca ttaatgaaat
    26581 tgaaatagag ggaataggaa atttgcacat agagtttaat agattaaagg aaaaacttaa
    26641 taaagaagga ttatttgacc ctaaatacaa gatacctata cctaaaatgc caaactcaat
    26701 tggcgttata acatctccaa caggtgctgt tataagagat ataataaatg taataaaaag
    26761 aagatatccg aaagttaata taaagcttta tccagtaacg gttcaaggag ataaatcagc
    26821 tgaagaaatc tgtgaagcaa ttagattttt caatcatatg aagaatgttg atacacttat
    26881 agttggtaga ggtgggggtt caatagaaga actttggtct tttaatgatg aaatggtagc
    26941 aagagaggtt tttaattctc aaattccgat aatatcagca gttgggcatg aaacggattt
    27001 tactatttgt gactttgtgt ctgatatgag agccccaacg ccatcagcag cagctgagat
    27061 tgctactcca tcattggatg atataaacta taaacttgga aacataaaaa gtaggatgag
    27121 taagtctctt acaaatcaaa tagagctaga ccaatatagg ttagaaacag tatttaataa
    27181 gataaataac tatttagatt cgtacacaat aaaagataaa gttatacaat tagacaaaat
    27241 atatgataaa ataatttttg gaatagaaaa taatttaaaa ttagaagatg aaaaattagt
    27301 aaaaattgga gcattactcc ataatttaag tccattagct actatggata gaggttatag
    27361 tataactcaa aaaaatggaa aagtaataaa tagtataaaa ggattaaaaa taaaggatag
    27421 tatagatata gtattaaaag atggaaattt agaatgtatg atagataaaa ttgaaaataa
    27481 agagggatag tatgaattta acctacgaag aagcttataa aagattagaa agtattttaa
    27541 gtgaattaga atctaaaaat gctagcttgg atgaatcttt aagcctatat gaagaaggta
    27601 taagccttta taaacattgt aataagcttt tagatgatgc taaattgaaa ataagtaaat
    27661 ttaatcaatt gggaatagaa gaagacttta aaatagagga ggaataaaat tggaatttaa
    27721 acaatgtcta aaagaaaaag caagttttgt agaaaaagta cttaaagaat atatgcctaa
    27781 agaagaaggg tatcaaaaaa ctgttattga agctatgaac tatagcttaa gtgctggagg
    27841 taaaagatta agaccaatac ttacattgga ggcttgtaaa atagttggag gtaatgaaga
    27901 tgaagctatt ccttttgcta ttgcaattga aatgatacat acatattcac taatacatga
    27961 tgatttgcca gctcttgata atgatgattt aagaagagga agaccaacaa atcataaagt
    28021 atatggagaa gctatgggga ttttagcagg agatgctttg ttaaactatg cttttgaagt
    28081 aatgcttgca ggctcaatca ataaagaaaa tccagaaaag tatctaaaag ctataaatga
    28141 aattgccaaa ggtgctggta tatatggaat gataggtggt caagtagtag atgtagaaag
    28201 cgaaaataaa caaattgaaa aagaaaaatt agattatata catatgaata aaactgctgc
    28261 aatgatggta ggctgtatga gagcaggagc tactataggt ggagcaaatt ctgaacaaat
    28321 ggaagaaata actaaatatg caaaaaatat cggattatca ttccagatag tagatgatat
    28381 actagacata gtaggagatg aagctaagtt aggtaaaaag gttggaagtg atatagaaaa
    28441 tcataagtca acatacccat ctcttttagg tttagataaa tctaaagaaa ttgcacataa
    28501 tttgattgat gaagcaaaga aaagtataga aaaattatct gatgatgtag attttttaaa
    28561 gggattggct gaatatataa tagatagaga atactaaaat atacagctaa aatcgaggag
    28621 ggaaaaatgg attttttttc ggagattttt aacaatgggg ctttgggaat aagtctgatt
    28681 gcttgtttct tagcacaatt tataaaaata tttactggta aggaaaaaag aattgagtta
    28741 tcaagaatac ttatttcagg aggtatgcct agttcccata gttcttttgt tacaagttta
    28801 gctacagtgg taggtattga aaaagggttt aattctactg attttgctat aattaccgtg
    28861 cttgccttaa ttataatgta tgatgcagca ggtgttagac gggctgttgg taaacaagct
    28921 actatattaa atcaaatggt ggcagatata cagcatggaa agcatataga gcagaagaaa
    28981 ttaaaagaat taataggtca tacaccacta gaagtttggt ttggagcttt acttggaata
    29041 gtaacagctt taatattgat gtaaattgaa aatttacaga ataaaggtga ggataatatg
    29101 tataaatatt tagataaagt aaattctcca aaggatataa aaaatatgag tatagaagaa
    29161 atggatttac ttgcaaagga tataagaaag ttcttagtaa aatctgtttc aaagacagga
    29221 ggacacttag cttctaatct aggagtggtt gagttaacat tagcacttca taaagtattt
    29281 gatagcccta aagataagat tgtatgggat gtagggcatc aatcttatgt tcataaaata
    29341 gttactggaa gaaaagattg ttttgtatct ttgagacagt ttaatggact aagtggtttt
    29401 ccaaaggaga atgaaagtcc tcatgatatt tttgatacag gacatagcag tacatctatt
    29461 tctatagcta caggtattgc ctgtgctagg gatataaaaa aagaaaacta tagtgttata
    29521 tctgtgatag gagatggttc tataacagga ggtatggcac ttgaagcttt aaatcaatta
    29581 ggatatatag ataccaatat gatagtaatc cttaatgata atgagatgtc catagataaa
    29641 aatgttggag gaatgtctaa atatctgtca agtattataa gaaactcaac agttgagaaa
    29701 atgacagatg aagttgataa aattttaaat gttacacaaa caggtgaaat tttatctaag
    29761 actgcacata gatttaaaga taaactaatg tatagctttt ctcctcagga ctgttcattt
    29821 tttgattcat taggtataag atattatgga cctatagatg gccataatac taaagaacta
    29881 atagatatat tgagaaaagc taaacacaaa aaagggcctg ttcttttaca tgtaattacg
    29941 aaaaaaggaa aagggtacag atttgcagaa gaacaacctg ataaatatca tggagtatca
    30001 aagtttgata taaagacagg tgttacatct gcaaaagtta agtctatgtc aattagtgtt
    30061 ggagaaaaac ttgttgatat ggctaatagt aatgaaaata tagtagcaat aacagctgca
    30121 atgccatctg gtacagggtt aaacctgttt gaaagtgcat atccaaaaag atattatgat
    30181 gttggtatag ctgaacaaca tgcaacagga tttgcagcag gtcttgcaaa aaatggtatg
    30241 aaaccttact ttgctgtgta ctcatcattt ttgcaaaggg catatgacca agttattcat
    30301 gatgtatgca taacaaaaaa gccagttact tttcttatag atagagctgg attagttgga
    30361 aatgatggag aaactcatca tggtatgttt gatttaagtt acttaaattc tattccaaat
    30421 attgtagtaa tggctccaaa agacacaaga gagatggagc ttatgatgga tttgtcatta
    30481 aaattagatt gtccactagc aattagatac ccaagaggaa gtagttatta cctagataag
    30541 ggagagtatg gagagattgt actgggtaaa tatgaggtat tagatgacgg tcaagataca
    30601 gtcatacttt gtattggaag tatggtaaaa catgctttag aggctaaaga aatattatca
    30661 agagaaggaa taaatccgac aatagttaat gctagattct taaagccaat tgatgaaggt
    30721 atgttaaaag cattattaaa aaatcataaa aatgtagtta ctatagagga taatattgta
    30781 actggaggat ttggaagtag aataaataag tttattatag ataatgaata taacgtaaat
    30841 atacttaata tagcaatacc agaagaattt gtaaaacatg gcaatataga tgaattatat
    30901 gactttgtag gattatcgcc aaaaagtatt gctgataaga taaggaaatt agttattgag
    30961 taaaaatcta atgtagatat tgtaaattaa aatttatata aaatatagaa tttatcaatt
    31021 agaataaata tcaagtgtgt tacttcaagc tactttagtt atcattaaag tagcttttaa
    31081 cagaaaggta attgtatgaa aaaaagaata gatttattat tagtagaaca agggtacttt
    31141 gaaagtagag aaagagccaa aaaagcaata atggctggat tggtttttgt tgataatcaa
    31201 aggtgtgata aggctggtac agaagtaaaa gaagattgta gtatagaagt aaaaggaaat
    31261 ccaataccat atgtcagtag aggtggttta aagcttgaaa aagcaatgaa aaactttgat
    31321 ttgactattg atggaaaggt atgtatggat ataggggcat ccacaggtgg ttttacagat
    31381 tgtatgctca aaaatggagc aataaaagta ttttctatag atgttggtta tggtcaactt
    31441 gcatggaaat tgagacaaga tgatagggtt gtgtgtatgg aaagaaccaa tataagaaat
    31501 gtgacaatag aagatacaaa gcagtttgct gattttgcgt caattgatgt gtcttttata
    31561 tcgttaaagt tagtacttcc aaaggcaaag gagttagtac gtcatgatgg ggaagttgtc
    31621 gctttaatta aacctcaatt tgaagcagga agagaaaaag taggtaaaaa aggagtagtt
    31681 agagaaaaat ctacacatat agaagttata aaaatgattt ctgatttctc agtcgaaaat
    31741 ggatttgaga ttttagggtt agatttttct cctattaaag gtcctgaagg taatatagaa
    31801 tatctaattc atttgagaaa tggaaatgaa ggatatgaat ttgatggtga gacatacaat
    31861 aacaaaatag tagaagttgt tgaagcttct cataatttag acaaataggt taaaaggggg
    31921 cttagatttg atcctcgaat tatatatgaa aaattgtgcc ttagttgaag aactaaggtt
    31981 gaatattgat aaaaacttaa atatacttac aggtgaaact ggttcaggaa aatctataat
    32041 aatagatgca cttggacttt gtttaggtga taaatatgat aggtcttttt taagaaaagg
    32101 aacagataag ggacttgttg aggctgtatt tttttcagac aatagatatc ttaaaaaaat
    32161 attagaagaa aatgatataa gtatggaaga tgataattta ttggtaataa ctaggttgat
    32221 atactcagat ggtaagagta ctgctagagt aaatggaagg actgttaaag tgtctcttct
    32281 taaagaaatt gcatctacac ttatagatat acatggtcaa catcaaaatc aagctttgtt
    32341 taataaagat actcatttaa aatttctaga cctttttgga gaaaatgaat tggaagagtt
    32401 taaaattgct tataaaaatg tgtaccataa gtatagtgaa gtaaaaaaag ctttaaattg
    32461 cttgacagaa aataaagatg agatgcaaat tcaacgagaa atagatttat taagatttca
    32521 aataaatgaa attgaagctg caaatctaaa taagaatgaa tatgaagatt tattaaaaca
    32581 aagagaagtt tatagaaata gtgaaaagat atataataat ctaaattcga gttatagcaa
    32641 gttacataat ggtgagtata atgttattga cctaataggt cttgcatcta aggaacttaa
    32701 tgacatatct aaatatgaca gtgttttaag tgaatatagt gatactgtag aaagaatcat
    32761 gtatgagtta caagacatat ctggtgagat aagaaattac aaagataata tagactttga
    32821 accttatgag ctagagcaga ttgagcttag aatcgacgag attaataact taagaagaaa
    32881 atatggagac agtatagatg atatatttga gtattatgga aaaattaaag atagattaga
    32941 tgaaatatta aacagagatg aaagagttga acagttaaga agcaaactga tgaatataga
    33001 agaagattta aaaataaaag ccagtaaatt gactaaagca agaatagaag tagcaactac
    33061 tttagaaaaa atacttcttg atgaattaaa aagcttaaat atgaaaaatg taatgttcaa
    33121 ggttaatttt gaaaaaggtc catttacttt agatggagta gatgatattg aatttatgat
    33181 atcttttaac ttaggagaag atattaagcc tatatataaa gttgcatcag gtggtgagat
    33241 gtccagattt atgttagcgt ttaaaaccat attggctgat atagatgata ttgatacatt
    33301 agtgtttgac gagatagata ctggaataag tgggatagca gcacagatag ttggtgagaa
    33361 attgagtgat atagcaaaga aaaaacagat aatatgtata actcatttac ctcaaattgc
    33421 tgcaaatgca gacactcatt attgtataga gaaagatact tcaaataata ggacgtttac
    33481 caatgttagt aagttaaatg aatctcaacg aaaaaatgaa attgccagac ttatagcagg
    33541 taacaatata acagaaaaaa ctattgaaca tgcaagtgaa attattgagt tggctaaaaa
    33601 atgttagtga aaaacaagca ttttaatagg tgtaaagaat ttttatagaa atgctttaca
    33661 tctatttttt atgatgaaat tattttaaaa tatctagtgc ctatattgaa gaaaagtagg
    33721 tttgaaaatg gaatgaactt tgtataaatt tgtaactttg aaatttctaa atgcttaagt
    33781 ataatattat ttattaaggt agtagtgagg ggaggcataa aatgaataaa aaagatacta
    33841 tgcacttaat ttttataatt gtaattttta ttttattatg gtatattgtg gtagcagtaa
    33901 gtgagattaa taaaaatatt gaaagttgta gtaacaaatt agattatttg tcacaagagc
    33961 ttatgagtac taagtcggat attagtaata ctataaatga agaaatgtca aaaagctata
    34021 taacaaaaag tattgactta aaagtgaaaa aaatcgagaa aaaagaatgt attattgata
    34081 tagatgttca attaagtaaa cttggaaaaa atggaaaagt attttttatg tataaagaag
    34141 atagtgataa atggaatgaa actgagatga caaaacatgg agaattatca tatgcatgtg
    34201 aaattaagat aaatacagga agtgaatatg actataaagt agttacaagt ggagctatat
    34261 ctgaaagttc tgatgtgcaa aaattgacta aatctgatta tatgcctgaa caacccatat
    34321 ttaattctgg gataagagat aatagagaat actttattga aatagtagaa aatagtaatc
    34381 ttttcaatca tgagagtgaa aagtcaaaaa ataaagttta tgataatatt agattagaaa
    34441 aagtagatat tattgtaaat gaaggaaaaa aagacactat atacaaagcc aaattagctg
    34501 aaggcttaga tgatggcaaa actaataatt caaatagaaa tcaagtgaat tatgaagtaa
    34561 gttttcctaa aagggatatt gatgacataa tttatattaa agctaaactt acatacaata
    34621 atggagtaga atatacaaag gatataactg aagatataac tatgggatta caagatttag
    34681 aaaaataaat acatatttaa ataaatattc aaatagattt gttcaataaa atagagtata
    34741 aataaaactg tagtagtttt acttatactc tattaaaaaa ttataatttt aattccatat
    34801 atctagcccc ttcaattgga ttataagtat aaggttctat atcataaaaa ccaaactttt
    34861 cgtataatcc ttgtgcactt ttcatgcttg gaagagtatc taatctcatg tgtgtatatc
    34921 caaatttttt agcttcttca atgatttctt ccaatagtat ttttcctatt tttaatcctc
    34981 taaattgatt tcttacatat aatcttttta attcacatat tttaccttca agttttttta
    35041 aagcaacaca acctgccaaa ttttcatcaa caaatgccaa tatcaatgag cctgaaggtt
    35101 ttttatattt tcctggaagt gtttttaatt cattattaaa gtcttgaaaa cataagtcta
    35161 tatttagaga atttgaatat tcagtaaata gaagttttac gttttcaagg ttttctaaat
    35221 ctttttcttc aacaaattgt aaagttatat tttgagccat tttatccgcc attatataca
    35281 catgtttata taatgacttc acctccttga tatatgtata tttttcttaa tatcaattag
    35341 attttaatac cattactttt tcaccaccat agtcaagctc tccattaaac tcaaatccaa
    35401 aatctttata aagcttaata gccattttat tgtcttcgaa tatactaaga tatagttcat
    35461 cacagttata ttcattttta agatgattta caagaaaatc taatgatgct tttccatatc
    35521 ctttaccttg atattttgaa tcaatcataa atctatcaag ccaaactcta tcatcttcta
    35581 aacgaccata catagcaaat cctattaaag aattttcatc atatattcca acaggtaccc
    35641 aagctgttat aaatttagat tcagcaatag aaagtgcatt gctttctata taatctaact
    35701 gacttttatc tacagacaaa ctaactaaat catgccaatt atttatgtct gcaattctta
    35761 catttaattt caagatatta ccgcccttca aactttttgt tacaatctaa tactatcata
    35821 ttatgtatta aaaatcactt attaagattt taaataaact ttttaaatga ataaattctc
    35881 aggaaaaagg aattataaat acataataat acagcatagg agtagatgga tatatgaaca
    35941 aaataaataa aaatttcaaa aatatttgga taatattagg agctgtatcg atttttacca
    36001 tggttataat ttttaataaa aattgtaatg ttaataaaga gactcaaata gaagctttta
    36061 caattaagaa tcatgataaa aaatttgtat atcctatggg aaatataatt ggagtaaaag
    36121 caaatacaga tggtgtattg gtcttaggat atgaggaaga ggatgtagac tatattggag
    36181 gcatacaaat cggtgataat atagtaaaaa tagataataa aaggataaaa aatagtcaag
    36241 atgtatcaga gattttgaat aagattaaaa aaagtaaagt tgaagtgact tttgagagaa
    36301 aaaataagta taaaacagaa gtaatagaga ctaaaaaaga aaatggcaag tataaacttg
    36361 gattatgggt aagagataaa atatctggaa ttggaactat gactttttac gaccctagta
    36421 tggaaaagtt caaagcaatt ggtcatgcca taaaagattc ggatactaat gaactattaa
    36481 agataaagca aggatatata tataagccag aacaattaaa aatagtaaaa gctagtaatg
    36541 aaaaagtagg taaaataaaa ggagatttta atgacagcaa tttaatgggt aatttctcaa
    36601 ataattcaga gttaggtata agtggtaata ttacagaaaa ccataataaa gagtttaatg
    36661 tagcaaataa agaaaagcaa ttaatagaag ttggaagacc gcaagatgta aaaataggag
    36721 gtgcagtaat tctttttgag gataaaaaca aaaatataac aagctatgat ataaaaatag
    36781 aaagtatagt atatgataaa ggaaattata gagatatggt aataaaagta gtagatgaca
    36841 agttattgga atacacagga ggtatcgtac aggggatgag tggagctcca ataatacaaa
    36901 ataataaaat tattggtgca ataactcatg tttttagaga taatccgaaa aaaggatatg
    36961 gtatttttat agatgaaatg ataaaattgt aggtgaggca ttaaaaaatt ttattatttt
    37021 atcaattatc taggaggaat ataattttgg agtgtcgaat atgctttaga gtagataatt
    37081 aggaagcaat tgtgtaaaaa gtttagtttt ctgtaataag aagatgtttt ttaatggggg
    37141 gatttttagt ggaaaaaatc aaaatagttt tagcagatga caataaggat ttttgtcagg
    37201 tattaaaaga gtatttgtct aatgaagatg atatcgatat attaggcata gctaaggatg
    37261 gaattgaagc attagactta gtaaaaaaga cacaaccgga cttattaata ctagatgtaa
    37321 taatgccaca tctagatgga ttaggtgtaa ttgaaaaatt gaatactatg gatataccta
    37381 aaatgccaaa aataatagta ctatcagcag taggtcaaga taagataact caaagcgcaa
    37441 taaatctagg agcagattac tacatagtaa agccatttga ttttgttgtg tttataaaca
    37501 gaattagaga gctagtttct aatagagtta cacaagttga gccaaaacct agaccagttc
    37561 aagagactca aatgactaga agtgattttg ttaagaatgt aggaaatata gagacagaaa
    37621 tcacaaatat aatacatgaa ataggagtac cagctcatat aaaaggatat ttatatttaa
    37681 gagaagcaat aaaaatggtt attgataatg ttgagctttt aggtgcagta acaaaagagt
    37741 tatatccaag tatagctaaa aaattcaata caacaccaag tagagttgaa agagcaataa
    37801 gacatgcaat agaggttgca tggagtagag gaaaagttga cacaataaat caattatttg
    37861 gatatacggt acacaatact aaaggaaaac caactaattc agaatttata gcaatgattg
    37921 ctgataaatt aagactagaa catagtatgg ttaaataaac aagacataaa aagtaaggct
    37981 ttttaattaa ggcattggct ataaatgcgt attacaagca gcgaaacggt ataaccacta
    38041 gggttatacc gtttcgctat tttaataaat ataaaaattt tatttattat ttgcttacta
    38101 tatcaatata ataattttat tatactatgg atatagtatg tgtctttaca agttgtaaac
    38161 tgacagtggt ttatttttta atataaatat tgactttgat gcaggtaaac tttgtatttt
    38221 taagcgtatt atggaatatg ttaaataaaa aaatgatgaa atatagtatt gtaaatgcca
    38281 aagatgcaaa acaaacttaa aacatttatt ttattgttaa gtaatgctat aatataatgt
    38341 gattttaata atgatagtgg aggtttaaat atgagagtcg aggcccctat aaaagtagat
    38401 cgaaaaacca aaaaacttgc taaaagagtt gaaagtgggg aaatagcagt tataaatcat
    38461 atagacatag atgaagttgc tgcaaactct ttagtagaag ctaaaataaa acttgtcata
    38521 aatgcggctc cttctataag tggtaggtat cccaataaag gtccagggat attaactgaa
    38581 aataatatac taataattga taatgttgga gaagagttat ttgaagaatt aaaagaagga
    38641 gaaactatag aagttgtaga tggaaagata tatagaaaag gaaaattttt aggtgcagga
    38701 gaagttttag acaagtatga ggtttcacac caaataaagg ctgcctatga gaatttagct
    38761 gtagaattgg atagatttat agataatact atagattatg ctaaaaaaga aaaaggattt
    38821 atacttggag aagtagaaat tccaaaagtg aaaactaatt atgctaataa acatgtattg
    38881 atagttgtaa gaggtcagga ctacaaagaa gatttaagta ctatgctttc ttatatagaa
    38941 gaggtaaagc cagtattggt tggtgttgat ggaggagcag acgctttaat tgagtttgga
    39001 tatactccag atgtgattgt aggtgatatg gatagtgtaa gtgatgaagc tttaaaaaag
    39061 gctagtgaaa tagtagtaca tgcttataca gatggaagag caccaggtct taaaagggtt
    39121 gaggaattag gcttagatgc agttgtattt cctgcaccag gaactagtga agatatagct
    39181 atgcttatcg cttatgagta taaggctgag ttaatagtag cagttggtac acattcaaat
    39241 atgattgatt ttcttgaaaa aggaagaaaa ggtatggcaa gtactttttt agttagacta
    39301 aaaataggtt caaagcttat agatgctaaa ggcgttaatt tgttatacag aagtaaatta
    39361 aaaataaagt acatatgggc attaatagca acagcattat ttcctgtgtt ggtagttgca
    39421 tcattgtcac caggagttca acagttcata caactaatgc aattaaagtt tagagtatta
    39481 ttacagatgt aaattttagg aggataaata tgcatattaa tatgaaatat tatatagtca
    39541 ctataggggc tatatttata gctttgggta taggcatgtt agtaggattt aatttaaata
    39601 ataatcaaca attaagtgaa caacaagcaa acataataaa tgatttggat gataagtttg
    39661 atatcttaaa agaaaaaaat gataaacttg acagtgactt agcaagtgta aatagagatt
    39721 atgaagaggc agttaatttt ataaataaaa atgtagataa gattttagct ggttcgctga
    39781 atggtaaaag tataggaata atatctgcta atgaaaatga cgattataca aaaaatatag
    39841 aagacataat aaataaatct aatggaagca ttgcatttaa tataatatta aaagaaaaca
    39901 taacaaatcc agaaaagtta aaggaaatgt caactaaact aggtacagat gtaaaaaatg
    39961 caaatgatgc agttaattat ataattgaca ctcttaaaaa ggaagatgca agtgacattt
    40021 taacatatct tcaagaacta gatgtaataa agtttaattt tattggagat acatatctaa
    40081 aatatgattc cgttgtaata gctggtggaa atgatgcaaa agacagtaca aaacagtttg
    40141 aaaaaataga aaaatttgta gtttcaaaac ttaaatcaga gaataaatat ttagtagaag
    40201 ttcaaaatac aggagttaaa acttcttatg tagaactata ttctaaaaac aaagttgcaa
    40261 ctatagacaa tattgatgaa ggtataggaa gtatatctct agctattctt ctacaacaag
    40321 gtaatatagt tggtaatttt ggtagattgg atactgcaac ttcattgttg ccatctatta
    40381 aatagaaata attttaagga gagaaaaaat ggattcttat ataagtataa taataccagc
    40441 ttataatgaa gaaagtagaa taaaatatac attagaaagc atattggaca ttaagcaaat
    40501 aaatgaaata atagttgtgg atgatggttc aattgacaat actgctaagg ttgtatctga
    40561 gctagaaaat gaaaagatta aattttttaa gttagataaa aatagaggta aaggatatgc
    40621 tttaaattat ggtatcaaat tagcaatgaa aaatgcagat attataggtt ttctggatgg
    40681 agatttagga agttctgcta aagaagttga aaaattaata gtacctatat taaatggtga
    40741 ggcggatgtt acaatagcca agtttcctcc tgcaaagaga aaaggaggtt tgggatttgt
    40801 aaaaggttta gctaaaaatt ctgtgtttga gatgactggt gttgagttag atgcaacttt
    40861 atcaggtcaa agaatattta aaagagaagt tttagaaaag tttgatgaaa taccttttgg
    40921 atatggagta gaagttggaa tgaccattga tatattaaag tatggattta aaattaaaga
    40981 agtattagta aatatgactc ataatgaaac aggaagaaat ttaaaaggtt ttattcatag
    41041 agggaaacag tattatcaca taaaaaaggt tttaggacaa aaaaagaaag aatggaggta
    41101 atatcttagt gaaagattat atactttata ccattttatt attaactgga ttatttggaa
    41161 cctatgctgt aattccacta tttaaaaatt tattgataaa tggaaatgta cttagaccaa
    41221 actataaaaa agatatgata ccagtaagta tgggaattgt atttttgcct atgattgtaa
    41281 tcaatggaat aatacttgct ttctttacta cagaatttaa ggatttagcg tatatattta
    41341 tgtttatgtt tggtatgata tccatgtttt ttgcagggat actagatgat gtcattggaa
    41401 atagagatgt aagtggttta aaggggcatt ttaaaagttt atttaaagga agtcttacaa
    41461 caggaggttt taaagcttta tttggtggat ttgtagggct tatagtttct gttgctatat
    41521 caaaagacat aattgatata atagtaaata cactaataat agctctatca actaatttaa
    41581 tgaatctttt ggatttaaga ccaggaaggg caataaaagc atatttattc ataatggtaa
    41641 ttatattttt aacattgaca ggatttgttc aagttcttcc attacttata gtaccaaatg
    41701 tacttgcata ctttaattat gatttaaaag caagggctat gatgggagat actggttcaa
    41761 acgtccttgg aatatctatt ggaatgctca tttcttttgg gtatccattt aacattagat
    41821 taggatggtt aatatttgta gttattatgc atatattcac tgagaaatac tcattaacta
    41881 agataataga aaaaaataaa ctattaaatt ttatagataa attgggaagg tagtattatg
    41941 ataagcaaaa gagtggggat tgttgaatca atagtaagtc aaactgaaac actagatgat
    42001 ataagagtaa atataaatgg agaaatacaa agggcatata attatcccaa aatatctgga
    42061 actataaata taggtgacga agtggtttta aatacaacag cagtagaact gagcttaggt
    42121 acaggtggat accattttgt aataactaat ttaaataata tagaatctac tttaactgaa
    42181 ggtggacata taatgaaact tagatatacg cctcttcaaa taaaagtgga ttctgttgag
    42241 gaacaagaaa gtatttatca tgataaattt gcgaatttta aaggattaga aggtctttct
    42301 gttgtagtag ggactcttca tagtatgtta actccatttg cagcttcatt taaaagaaat
    42361 aatccaaata aaaaattagt gtatataatg actgatggag catctcttcc aatttattta
    42421 agtaaaaatg tggatacttt aaaagaaaaa aaattaatag atagtacaat aactatagga
    42481 aatgcgtttg gtggagatta tgaatgtata aatatatata ctgcattaat tacagcaaaa
    42541 gaaatactaa aagcggatgc agtatttgtc agtatgggac ctggaattgc tgggacagga
    42601 acaaaatatg gatttacagg gattgaacaa ggctctattc ttgatgctgt aaaaaaactt
    42661 gaaggaagag caattgcaat tccaagaatt agttttgctg acaaacgaga aagacatcaa
    42721 ggtatttcac atcatagtat gactgtattt aaagaaattg taaatgtaaa tgtagacata
    42781 ccaataacta tatatgatga tgaaaaattg aactttataa aagagcaaat caatttaaat
    42841 ggtttagaca aaaagcataa tataatttat atagataata ataaaacgaa agaggattta
    42901 gactatttta atctaaaagt aaagagtatg ggaagaaatt atgaacaaga tgaggcattt
    42961 ttcaaagctg caagcacagc agcatattac ttaatggagg tttaagatgg tattagaaga
    43021 aaaaactatt agtagtgata gggtttatac aggtaaagta attactttaa aggttgatac
    43081 tgttgaaatt ccagggcaag gatatcaaaa aagggagtta gtagaagttg gtggagcagt
    43141 aggaatagtt gcaatcacag atgataataa agttgtgtta gttaagcaat ttagaaaacc
    43201 tatagaaaag ccaatctttg aaattccagc tggtaaacta gaaaaaaatg aaagtccaaa
    43261 agaatgtgct gaaagagaat taaaagaaga aacagggtat agtgcaaaaa atattaagct
    43321 aatacataaa tttttcactt cagcaggttt ttctaatgag ataatgtttg tatatttagc
    43381 aacaggtctt actccaggag agaataattt agatgcagat gagtttttag atgtctatga
    43441 aattgagctt gaagaagcat ataacatggt attaaaaaat gacgttgaag atgcaaaaac
    43501 atctattgga ttattattag taaaagatat gtttaaaaat taatatttat aaaatgaata
    43561 accctttgtc ctaaagcata ttattttata aaggacaagg gggaattaaa ttgagaagaa
    43621 catatagaaa aaatgatttt agagaattaa atagacaaat aattattata ggattgttgt
    43681 ttatgatgtc tattgttgtt gggtcttaca taaataaaat attgccaggt tctagtaaca
    43741 atatattaaa caatataaat cctgctgtag agtattataa tctaaatata agcataaaag
    43801 atacagtgat acaaaattta aaatcagatg caatctttat gggctcaata gctctattaa
    43861 gtctatttgt agtaacaatt ccagcagtgt tggtagcttt tgtattaaaa gggatgtcaa
    43921 ttggttatac aataaatagt tgtatattgg cattaaaatt taaaagtata aaaatgattt
    43981 taataatatt atttaaaaat ttgattatta ttccatgtgc aatcatatta gcattaatta
    44041 gtttaagtta ttttaaagaa atggtttatg aatttaaaaa gaaaaataga aaaaatatgc
    44101 aatttttaat taagaggtat atactaaata ttataattat aatagcacta tcattggggt
    44161 tgcaattaat attaaatact gcaagtattg gtattattaa gttcctagct aaataaagga
    44221 gtttataatg gatattatag agggatatat agattacata aaagataaaa aaaaattatc
    44281 agagaataca gtggcatcat actttatgga tataaaaaaa tatatggaat atataaatca
    44341 aaaaagcatt aaactagtag atatagtaga aaatgatatt ataagttact taataaagtt
    44401 ggaaaaagat aatgtatcaa tagctacaat agcgaggatg atatcctcta taaaatcttt
    44461 tcatgattat ttatttttaa atcatatatg tacaaataat cctgcaaagg atataaaaaa
    44521 acctaaaata aaaaaagaaa atataaatat acttacagaa gaagaaattg aaagactatt
    44581 aaattttcca aagttaacaa cacctaaatt gattagggac aaggctattt ttgaagtatt
    44641 atatggtact ggtataaaag tatctgaatt agtagagatg aatatagagg atattgacct
    44701 agatattgat tatatatatt gtaattcatg ttgtaaaaat caaagagtga taccactttg
    44761 tgatattaca aaactttact tagaaaagta tctaaaagaa gcaagaccta aaatggctgt
    44821 agaaggtgaa aaatcattat tcgtaagttc attaggtcaa aggtttacac gacaaggtct
    44881 ttggaaagtc ataaaaaaat attctaatct agccaatata gataaaaata taaatcctac
    44941 aatgcttaga cattcatttg ctattcattt gttaaatgaa ggtgctaaca ttgctgttgt
    45001 aagtaaaatt ttaggaaatg ttaatttatc tagtttgcaa gtatatttaa atcatataga
    45061 taaaaacgtg agaagagaaa taaaggagaa acatcctaga aatgatgttg atgtagaatt
    45121 acaaaaagca gaagctaaat agactatata gcaaatttta taatttagaa ggagcatatt
    45181 tatatgagca gagtaatttg gattgtaatt gatagtgttg ggataggagc attgcctgat
    45241 gctgaaaaat ttggtgatag taaggatgta agtacccttg gaaatatatt taaggaatat
    45301 ccagatattc agattcctaa tatgagaaat ttaggtatag gaaacataga tggtatagat
    45361 ttttttgaaa gtataaaaga accaataggt tgttttggaa agtgtaagga aatgtctcag
    45421 ggaaaagata ctactacagg tcattgggaa atgactggta taatagttga caaaccattt
    45481 aagacatttg aacatggatt ctctaaggag ataatagaag agtttgagaa aaaaactgga
    45541 agaaaagtag taggaaataa accagcttca ggaactgtaa taatagatga gtatggagaa
    45601 catcaaataa aaactggaga tgtaatagta tatacatcag ctgatagtgt atttcagatt
    45661 gctgctaatg aagaagtaat tcccctagaa gaactctata atatgtgcaa aatcgctagg
    45721 gaaataatga tgggggataa tgcagtagct agagtaattg ctagaccatt cataggtaag
    45781 aaaaaaggtg agtttgtgag gacttcaaat agaagagatt attcactaga cccatttgaa
    45841 ccaacagttc ttgataatat aaaagaatca ggacttgatg ttttagcagt tggaaaaata
    45901 gaagatattt ttaatggaaa aggaattaca gatgctatcc acactaaaag caatatggat
    45961 ggagtggatg aaactttaaa ttatatgaaa caagataata aaggtctaat atattcaaat
    46021 ctcgttgatt ttgattctaa atatggtcat aggagagacc cagaaggata taagaaagct
    46081 cttgaagagt ttgatagtag acttcctgaa ataatggcta atatgagaga agatgatatt
    46141 ttaataatca atgcagacca tggaaatgac ccaacatata aaggtacaga ccatacaaga
    46201 gaatatatac ctgttatgat ttatggcaat aagattaaaa aaggttttaa tttaggagta
    46261 aaagatactt ttgctgatat tggagcaact gttgcagata tactaaatgt aaagttgcct
    46321 aaacatgggt caagctttaa agaagattta ttttaaacta taaaatgata aaaacttagc
    46381 ttaataggat ggagagattg gaatgtttga aaagatagca caaagtagta aatttataaa
    46441 ttctaaaagt gatataaaac ctaagattgg tttgatatta ggttctggac taggtgattt
    46501 agcaaatgat atagaagacc ctgtcgcaat taaatatagt gaaattccaa acttccctgt
    46561 atcaacagta gcaggacatg ctggacaact tgtaattgga aaacttgaag gaaaagaagt
    46621 tattgctatg caaggtagat ttcattacta tgaaggatat tctcagaaag aagctacttt
    46681 tccagtaagg gttatgaaag aattaggagt cgagattctt atagtaacta acgctgctgg
    46741 aggcgtaaat aaagaattta aagcaggaga tttgatgctt ataagagacc atataaactt
    46801 tagtggaagt aatccattag taggaaaaaa tgatgacaga tttggtgcta gatttccaga
    46861 tatgtcagat gcatattctt ctaaatatgt agatgtagtt aaaaactgcg ctaaagaatg
    46921 caatatagat gtcaaagaag gcgtttatat gttttttagt ggtccaaact atgaaacacc
    46981 agcagaagtg agaatggcac aaattttggg agctgatgct gtaggtatgt ctactgttcc
    47041 agaagtcata gtagcttctc actctaatat aggagttatt ggtatatctt gtataacaaa
    47101 tatggctgct ggcatcttaa atcaaccact aagtcatgag gaagtaatag aaactactca
    47161 aaaagttaaa tcagaattta tgaatttagt taaaacaacg gttaaaaatt tataagttaa
    47221 agcttattat agattttttg aggaagttta taataaaata gttaatgttt aatttgaaaa
    47281 gaattaataa aaagctgtct tgatatagaa agttatttct aataaaggac agttttttta
    47341 taatactaca acatttgaaa gtgttttaaa gtttaatatt tatttaaatt attataggtt
    47401 agaatggaga tttcaattat gagaatgtat gatattataa aaaaaaagag agataaccta
    47461 gagcttagta aaaaagagat aaactacttt atagaaaact attcaaaggg agatatacct
    47521 gactatcaag cttctgcact attaatgtcg atttatttaa ataagatgaa taaacaggaa
    47581 actgtacatc ttacagaagc aatgatgaac tctggagaca tgataaacct atctgagata
    47641 gatggaataa aggtagataa gcatagtact ggtggtgttg gagataagac taccatagct
    47701 cttatacctt tggttgcttc atgtggagca ccagttgcaa agatgtcagg tagaggtctt
    47761 ggtcatactg gtggtactat tgataaattg gagtctatac caggtttttc gacagaaatg
    47821 gagataagta aatttattga ttctgtaaat aagtctaaaa tagcagtttg tggtcaaagt
    47881 gcaaaggtag caattgcaga taagaaaata tatgctctta gagatgttac agcaactgtt
    47941 gataatatat cattgatatc atcaagtgta atgtctaaga agttagcttc aggagctgat
    48001 gcaattgtgc tagatgtaaa aactggtgat ggagcattta tgaagacatt agaagagtct
    48061 tttgagttag ctaaatctat ggttgatata ggtactggta tgaatagaga tacaataggg
    48121 attgtaacag atatggatga acctttaggt tttgcagtgg gaaactcttt agaagttata
    48181 gaagcaattg agacattaaa aggaaatgga cctaaagacc ttgttgagct atgcgaagta
    48241 ttaggagcat atatgttggt tttatcaaag gtagcacatg attttgaaga tggtgttgaa
    48301 aaaataagag aatcaataaa atctggtact gctatacaaa aactcaaaga gtttatagag
    48361 aatcaaggtg gtaataaaga tgtagtagat gattattctt tattcaaaaa atcaaagtat
    48421 aaatatccag ttaaatcaac taaatcaggt tatataagca aaattaaagc tgaagatgtt
    48481 ggtgtatcgg ctatgattct tggagcaggt agagaaacta aagatgatat tttagattta
    48541 tcagcaggga taattttaga aaagaaagta ggagattatg taaatgaagg tgaagtttta
    48601 gcttacatgt attataatga tgaacaaaag ctttcattgg caaagggaag atttattgat
    48661 tcctactcta ttgtagattc aaaaatagaa aaaaataaat taatatatgg tattgtaact
    48721 aaagaagaga ttaaaaagtt ttagaaaatt ttagaaaagt gattataaaa ggcctaacat
    48781 gtcaaaaata atgttaggtt ttttattttt tgatagacta taaattatac caaaaatata
    48841 tattttaaaa cattttttac aatataaata agtttattct acaagatgat tgaaaattaa
    48901 agtatctagt tttataatag aaaatggata aatatacata aggagtagat tatattatga
    48961 actttaaaga aaatagattg aaaatagcag tattaataat tgttattttg attatcttag
    49021 caggtatatt tgtgtttata caaataggtc cttatgataa aaataacaaa aaagatgtaa
    49081 ttatcgacgt tccaagtgga gcatctgttg gaaaaatatc tgacatatta tatgaaaata
    49141 aattaataaa gaatgaacta ttatttaaac ttttggtaaa ggttagcaat aaagctccgt
    49201 ctataaaatc aggaacatac ttacttaatc aatcgtattc aaacaatgat ataataagtc
    49261 ttttagtttc aggtaaaata tatcaggatg gtataaaagt gactattcca gagggagcta
    49321 catctaaaga aattatagca atgttagtaa gtaaaaattt aggagataaa gctacttttg
    49381 aaaatttaat aaaaaaacct caagaatttt atgataaatt tccatatttg aaggaagatg
    49441 gaataacctc tttagaaggg ttcttgtatc cagaaactta ttattttaat tcaaagaaac
    49501 aatcagaaga agatatttta agcgaaatgt taaaagtatt tgacagtaaa tacacggaca
    49561 agtttaaaaa gaaacaaaaa gaattaaata tgactcttca agaagtaatg gaaatggcat
    49621 caataattga gaaagaagct gttcttgaca aagacagacc tataatagca agtgtgtttt
    49681 ataatagact taaggtaggt atgcctcttc aatcagatgc tacaatacaa tatatatttg
    49741 aagagagaaa gaaaattgta acatatgatg atttaaaaat tgattcacca tataatagtt
    49801 ataaaaataa gggattacct cctacaccaa tatcaaatcc tggtataaaa tctattgaag
    49861 cagcattata tccagagaaa acagactatt tatattttgt agctaagatt gatggtggaa
    49921 ataattatag tacaaactat caagaccatt taaaatacgt taaagaatat aaagaagcaa
    49981 gggataaaca atcaaaagat acaaaagcaa caaataaaga aaatacaaaa aaataaacca
    50041 tgattataaa aagtctaaca gggtaagaat aatgttgggc ttttttattg aaaattgtta
    50101 ttcttagatt tataata
//
LOCUS       DACULQ010000005        48453 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.005, whole genome shotgun sequence.
ACCESSION   DACULQ010000005 DACULQ010000000
VERSION     DACULQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.005"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..503
                     /locus_tag="JA154_01170"
     CDS             <1..503
                     /locus_tag="JA154_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7994467.1"
                     /translation="IDRLISKYKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEK
                     DIDEIGKLLNRSDYKVSKNYIEERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLA
                     MVVSVSTDKRYRGKGLASYMVYNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINE
                     WTILFK"
     gene            complement(600..1424)
                     /locus_tag="JA154_01175"
     CDS             complement(600..1424)
                     /locus_tag="JA154_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HAT7994468.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            1645..4764
                     /locus_tag="JA154_01180"
     CDS             1645..4764
                     /locus_tag="JA154_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAT7994469.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FLEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKYRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            4782..5381
                     /locus_tag="JA154_01185"
     CDS             4782..5381
                     /locus_tag="JA154_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H"
                     /protein_id="HAT7994470.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(5638..7776)
                     /locus_tag="JA154_01190"
     CDS             complement(5638..7776)
                     /locus_tag="JA154_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAT7994471.1"
                     /translation="MIYMPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGD
                     FTPVCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIE
                     DRDMRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDA
                     LQTSDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYN
                     DEEVSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEK
                     KHTKNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYK
                     NYDYTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPT
                     NVDAQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGF
                     SDAALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLT
                     AIRDVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKH
                     QANLFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDY
                     INKNGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYD
                     QANILARTMSIGINDPEVLKEELEGIYGQF"
     gene            7991..8281
                     /locus_tag="JA154_01195"
     CDS             7991..8281
                     /locus_tag="JA154_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994472.1"
                     /translation="MSGLKKILRILVISMMICFAMTFALVLINSVMGVHIGLDYYFLM
                     GSVLTVCLTIGIVLCNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            8447..9838
                     /locus_tag="JA154_01200"
     CDS             8447..9838
                     /locus_tag="JA154_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAT7994473.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            9953..11422
                     /locus_tag="JA154_01205"
     CDS             9953..11422
                     /locus_tag="JA154_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT7994474.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            11487..12080
                     /locus_tag="JA154_01210"
     CDS             11487..12080
                     /locus_tag="JA154_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAT7994475.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            12262..13608
                     /locus_tag="JA154_01215"
     CDS             12262..13608
                     /locus_tag="JA154_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein complex assembly protein"
                     /protein_id="HAT7994476.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMFYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            13790..14161
                     /locus_tag="JA154_01220"
     CDS             13790..14161
                     /locus_tag="JA154_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAT7994477.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            14154..14843
                     /locus_tag="JA154_01225"
     CDS             14154..14843
                     /locus_tag="JA154_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAT7994478.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            15363..16229
                     /locus_tag="JA154_01230"
     CDS             15363..16229
                     /locus_tag="JA154_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAT7994479.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            16471..17280
                     /locus_tag="JA154_01235"
     CDS             16471..17280
                     /locus_tag="JA154_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994480.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            17424..18488
                     /locus_tag="JA154_01240"
     CDS             17424..18488
                     /locus_tag="JA154_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAT7994481.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            18516..19235
                     /locus_tag="JA154_01245"
     CDS             18516..19235
                     /locus_tag="JA154_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitooligosaccharide deacetylase"
                     /protein_id="HAT7994482.1"
                     /translation="MTSWKKKTVYKCLIAVALFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            19259..19843
                     /locus_tag="JA154_01250"
     CDS             19259..19843
                     /locus_tag="JA154_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HAT7994483.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            19836..21194
                     /locus_tag="JA154_01255"
     CDS             19836..21194
                     /locus_tag="JA154_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase, component I"
                     /protein_id="HAT7994484.1"
                     /translation="MCNMIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSF
                     ISSQPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENL
                     PRTAVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEE
                     KKGIDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETE
                     LTSFELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGK
                     DKEEDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNH
                     LVSTVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYI
                     GFNGDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYY
                     EE"
     gene            21184..21927
                     /locus_tag="JA154_01260"
     CDS             21184..21927
                     /locus_tag="JA154_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAT7994485.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            21961..22662
                     /locus_tag="JA154_01265"
     CDS             21961..22662
                     /locus_tag="JA154_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994486.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKILNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            22688..23260
                     /locus_tag="JA154_01270"
     CDS             22688..23260
                     /locus_tag="JA154_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAT7994487.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            23312..24115
                     /locus_tag="JA154_01275"
     CDS             23312..24115
                     /locus_tag="JA154_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAT7994488.1"
                     /translation="MSLIVFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDM
                     VDQGADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEE
                     ALKLGVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVS
                     IEMAMSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGK
                     VLPVEPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            24126..24491
                     /locus_tag="JA154_01280"
     CDS             24126..24491
                     /locus_tag="JA154_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HAT7994489.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            24484..24990
                     /locus_tag="JA154_01285"
     CDS             24484..24990
                     /locus_tag="JA154_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAT7994490.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            24971..25756
                     /locus_tag="JA154_01290"
     CDS             24971..25756
                     /locus_tag="JA154_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAT7994491.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            26011..27801
                     /locus_tag="JA154_01295"
     CDS             26011..27801
                     /locus_tag="JA154_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAT7994492.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            27813..28985
                     /locus_tag="JA154_01300"
     CDS             27813..28985
                     /locus_tag="JA154_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087956.1"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; primary sigma factor of
                     bacterium; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAT7994493.1"
                     /translation="MSVENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETEL
                     DKDQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAI
                     EIETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEA
                     NLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAIT
                     RAIADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIM
                     KIAQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQ
                     KVLKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            29150..29839
                     /locus_tag="JA154_01305"
     CDS             29150..29839
                     /locus_tag="JA154_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7994494.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            29853..30950
                     /locus_tag="JA154_01310"
     CDS             29853..30950
                     /locus_tag="JA154_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAT7994495.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            31010..31585
                     /locus_tag="JA154_01315"
     CDS             31010..31585
                     /locus_tag="JA154_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT7994496.1"
                     /translation="MMKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAK
                     TLPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFS
                     DIWVGTPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAY
                     GILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(31645..32112)
                     /locus_tag="JA154_01320"
     CDS             complement(31645..32112)
                     /locus_tag="JA154_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HAT7994497.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(32498..32974)
                     /locus_tag="JA154_01325"
     CDS             complement(32498..32974)
                     /locus_tag="JA154_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAT7994498.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(33094..34185)
                     /locus_tag="JA154_01330"
     CDS             complement(33094..34185)
                     /locus_tag="JA154_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994499.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(34178..34567)
                     /locus_tag="JA154_01335"
     CDS             complement(34178..34567)
                     /locus_tag="JA154_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7994500.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            34759..35205
                     /locus_tag="JA154_01340"
     CDS             34759..35205
                     /locus_tag="JA154_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994501.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            35490..36194
                     /locus_tag="JA154_01345"
     CDS             35490..36194
                     /locus_tag="JA154_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7994502.1"
                     /translation="MLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSN
                     EYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGGDDYITKP
                     FRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFLTSVEYKL
                     LLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETV
                     RGIGYKWIGSENSVSI"
     gene            36172..37425
                     /locus_tag="JA154_01350"
     CDS             36172..37425
                     /locus_tag="JA154_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7994503.1"
                     /translation="MKIVFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMV
                     VRNNQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPI
                     ISKLVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKN
                     KNQEGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLND
                     LLYNDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHR
                     AMLSMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITY
                     KENPLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNG
                     DIYVNSEKGQGTEFHIIFHKMYDSD"
     gene            37505..38182
                     /locus_tag="JA154_01355"
     CDS             37505..38182
                     /locus_tag="JA154_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7994504.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            38183..40744
                     /locus_tag="JA154_01360"
     CDS             38183..40744
                     /locus_tag="JA154_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7994505.1"
                     /translation="MNLYTLLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINNSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIITIVSIIIVLLTIF
                     ISILLPAISASNISHLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            40935..42209
                     /locus_tag="JA154_01365"
     CDS             40935..42209
                     /locus_tag="JA154_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAT7994506.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(42370..45411)
                     /locus_tag="JA154_01370"
     CDS             complement(42370..45411)
                     /locus_tag="JA154_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HAT7994507.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            45618..45917
                     /locus_tag="JA154_01375"
     CDS             45618..45917
                     /locus_tag="JA154_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAT7994508.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(46155..48374)
                     /locus_tag="JA154_01380"
     CDS             complement(46155..48374)
                     /locus_tag="JA154_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAT7994509.1"
                     /translation="MKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRGNYA
                     VLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGDQLI
                     EQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEIIYT
                     VNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLTKVE
                     NSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDLKKF
                     LEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLSEKL
                     SNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVISGQ
                     QPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLDKAE
                     AKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMKKLV
                     SLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALLSTS
                     VHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTSMVF
                     NFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYAIGA
                     TREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLTGH"
BASE COUNT        19310 a         5206 c         8065 g        15872 t
ORIGIN      
        1 taatagatag actaataagt aaatacaaag atttttatga aaaactagat aataaatttt
       61 gtgtattaaa agaaataaaa gaaatagatt tttccaacat gaaggagtat aaaatagaaa
      121 atgctcagga aaaagatatt gatgaaatag gaaaactttt aaataggtca gactataaag
      181 taagtaaaaa ttacatagaa gaaagaaaag ttcatttaaa agagggaaat gtaagagcat
      241 attttattag aaatgatgat actatgatta gcactgtgtc aactggtatg gaaactagtt
      301 ttttggcaat ggtggtctct gtaagtacag ataaaagata taggggaaaa ggacttgcaa
      361 gttatatggt ttacaatttg agtaaagaat tgttgttaga aggaaaagtt ccatgtcttt
      421 tttataataa tgatgtagca ggtaaaatat atcataatat aggatacaaa gagataaatg
      481 aatggacaat tctttttaaa tagctataaa aaataaaaaa ctctatcgag aattaaagta
      541 tttataaata actatatttt tactataatt catgatagag tttttttaaa tttatgatac
      601 tattttttat tatttaaata atcaagtgtt ttaagtttat aaccttcttt ttcccaatgt
      661 gtaagaacct catctaatat ctcagtattt gttgatgaat ttggatgcag taaaactatt
      721 gcaccaggat gagttcttga ataaattttg tttttggcaa attcatgagt aggttgtttc
      781 ttttcatacc aatctacata agcaaaactc caaaatattg atttatatcc taaatcttgt
      841 gtatatttta aagattgttc gctaaattta cccattggag gtctaaaata cttaggcatt
      901 tcttttccag taacatcttt gtatgctttt tctacacttg taatttcttc tttgaatttt
      961 tcaaaatctg taatacctgc cattgatgga tggctttttg aatgattaca aactaaatgt
     1021 ccttcttttt ccatacgctt tattaactct ggattgcttt ttatataact ttcaactacg
     1081 aaaaattgag ctttagcatt gtgtttttta agcgtatcta ataattttgg agtgtttcca
     1141 ctttcatatc cagcatcaaa tgatagatat ataacttttt cattgggatt tcctacataa
     1201 taagctccgt attttttaaa aaaatcagct tcttttatag gtgatggctg tttaccatcc
     1261 tccctagggt taaaatacca atcatattca tgtgtgtcga gtgttggact agaaatttga
     1321 gtttttgttt tatctaatga tttaaaattt atactagcaa ttccaaaaag tattagagca
     1381 aatgctccta tcataatgta tttttttagg ctatcttttt tcacaatttc acctcttatt
     1441 taaaatttaa agtatatttc ttataatata gtttgaatat ttatagttaa aaatattcac
     1501 atatacaaat aataaatgaa agaagtttac tgtaagatat aaagttgata ttaattatct
     1561 taaataaagt gtatataaat gataaaatat aaagagaaat gattgtaaat aagttaggct
     1621 ggtttaactt gaagggatat aaaaatggat aatataataa gtgtattgaa tgatgttgtt
     1681 tttttagata tagaagttag tggattagat tgtttaaatt cagaaatttt agaagttgga
     1741 gctgtcaagg ttaaagactg gaaaatatat acctatgaat cattaataaa aaacaaattt
     1801 gaggtacctg tagaagtatt ctcagtatgt aaaaatttag ataaaaatga tttagaaata
     1861 gctaatgaaa tagaactggt ggaagataga cttgtaaatt ttgtagaaga tagtttcatt
     1921 atatgtcatg atttaagtct gaaaaaaaag tttcttgagt atcatatgcc aaagttaaaa
     1981 aacaaattta ttgatttaat tgaattagca gttattttag aaccatatca taaagattat
     2041 agcttagaat acttaaaaaa tactttaaca aattgtaatt ctaaagtgga aaatagagct
     2101 ttatcagatg ctattgatat aataaatatt gtaaattgcc ttttagtaaa atttaataac
     2161 tatgaaaaaa ctactttgga gccattgagt tttaaaataa attcatatct taaaaaattt
     2221 aatcttccaa catgggaatg gagcaaattt ttagaagagg caaattatga tttatcaaat
     2281 aatataaata taaaaaaaga atataatata tttgactcga aagaagaaaa gaagaaggaa
     2341 agagaaacct taaaaatttt aaatgaagaa gaaaaaaatt atgaagaatt attaaaatac
     2401 aaaactattt gggagaataa agaaggattt acatatgaat atagacctgg tcaatatgag
     2461 cttactaaaa caataagaga gttgtttaga aatagcgaag atgaagaaaa aattgcatgt
     2521 atagaggcac caactggtat aggtaaaagt gtaggatatt tattgcctgc aatattagag
     2581 gctagaatta ataaaaaaag attattaatt tctactgaca caaaggaatt gcagatacaa
     2641 ttaataaata aagatattcc aaatgtgtta gattctttgg gtctaaatgg aaaagtaagt
     2701 tacggatata taaagggaaa aaataactat atttgtatag atagactgga agcatatata
     2761 gatgattatg agtctcaaaa ccctactaaa ggagaaattt tatctttaat atttttgaag
     2821 agattagttg aaggtggcaa gtatggagat atagaagaaa taaattattt agtgtttgac
     2881 aacttcaagg aaataagtac tcacttaaga aatgtaagtt gtgacccaaa tatgtgcaga
     2941 cctaaaaaat gcaaaaaaga ttgtttatat aaaaacagaa tagaagaatt aaaagaagaa
     3001 catataacag tagtaaatca ttcactatta gctaaatggc catataagga tgaaaaacct
     3061 ttagaaaata taatagtaga tgaagcacat aatttgactg aaaaaggata tgatttcttt
     3121 tcaagcatta taaattcaaa gtctttaagg tatttattac aagaaattta tccttacgag
     3181 tttattcaaa atagtagctt tatatataaa aaatatagta gaaatatgag aaaaataaag
     3241 gcttttgata aattttataa tgtattaaaa attggaagag aagataaaca aaaaattgct
     3301 agaagtataa atctcattat tgaagagata gattcaatat taaagtttgg taattgtaat
     3361 gaatataata atgtaagtaa ttataattta agatgggaac tgaatttgca aatagacgaa
     3421 attgtgggaa aactaaaaaa ggatggtata gatactgaaa taagttatag agcctatagt
     3481 gaaaagataa agttgagttg tgagaaaata ataaaaaatt tagtttcaat aataataata
     3541 atttatagga atattgatga tgatagtata gataaagaag cagatatata taaatttgga
     3601 aaggcaaaga caagagattt agaggatata aagattatat ttgaaatctt tttagagtat
     3661 gatgaaaagg atgattatgc aaggatagtt gaaattgata agaattataa tgactttgaa
     3721 tttcgagtag tgccattaaa aatagcagat ttatttgaag aaaatatttt atctcaatta
     3781 gaaaaaggaa tatttttatc agcaactttg agtttatctg aaagtatgtc ttattttaaa
     3841 aatacattag gaatagacag agttaagaat gtggaaaaga ttatagaacc aatttttgat
     3901 tataaaaata gagtctctgt agttggattt tctgatattt gtgagtacag aaattctgaa
     3961 tttccaaatg agatgagtaa aattatatct aatatatcaa agattacaga aggtcataca
     4021 ttagctcttt ttaatagtaa agatagacaa gaaaaaactt atgaaatatt gaaaaaatac
     4081 ttacatagtt ttaacttaga aatttatgct gataaaaagg ggattagaca ccttaacgat
     4141 ttaaatagaa aatgtgtagt acttggctca aaaggatgtt ttgaaggcgt agacattcca
     4201 ggggatggtt tggtttgtgt aactttagac aagcttccga atctaaatcc aaaagaccct
     4261 ttgtatttta ctataatgaa gaagtatggt atagactact atactataaa ttatcctcaa
     4321 atgaccataa aggtaaagca agctatgggg agaattttaa gaagcaaata tgactatgga
     4381 tgttttgtga tttttgatgt aggaactaat ataagtgtgt taaagagatt agagaaagat
     4441 ttacacgatt gtaaaatttc aaaggttaat agcaatgagt tttatactta tataaggcga
     4501 catttaaata aatatagaag tttaatacta aaatctgtta tatttgatac tataaaagcg
     4561 ttgaatgttg atgctaaaat ggataacaat gatgttgata aggatattat aaaaaaagat
     4621 ataaatgaaa atataagaca aagagctgta aaaggagaag tgtaccatat tgatattata
     4681 aaaaaagata tgaaagtaaa atattttgat agaaactatc ttataaattt agatatattt
     4741 atgagagaag aagataaaaa ttaagagtgg gagtgatata tttggcaaaa caaaagtttt
     4801 atgctgtgaa aaaaggtaaa aatataggtg tatataatac atgggatgag tgcaaaaaac
     4861 aagtaaatgg attctcagga gctgaatata agagtttttc tacttttcaa gaagctaaag
     4921 aatatattga tgggtcagaa aaattgagtt ttcaagaaga taaggagttt atagaagcat
     4981 atgtagatgg aagttatgaa cattcagtaa aaatgtatgg ttcgggtgtt gtgattttaa
     5041 aaaataatga agttataaaa acttatagtg aaaaaggaaa agagaaaact ttagtaagta
     5101 tgagaaatgt agctggagag atagaggctt ctaaaattgc aatgcagtac tgtattgaca
     5161 ataatgttca aaacttgatt ttatactttg actatgaagg gattgaaaag tggtgtactg
     5221 gagtttggaa aacaaataaa gaaggaacta tagcatataa aaatttctat gacagtatta
     5281 agaataagct taatgtaaaa tttacaaagg ttaaagctca ttctggaaat aagtataatg
     5341 aggaagcaga taaattagct aaaaaagcta ttggtgtata ataaaaaaat atttatacat
     5401 aattacatgg tataagagta tatctaaatt cctactttat aattaaacaa cttgtaatta
     5461 aacaacttgt aattaaacaa cttataatta aataaatttt gattaaaaaa gcagattgta
     5521 ttatgaaaaa ataagtatct aatttataaa caattaataa tagtattaat tagatacaac
     5581 ttatttaata taataaaatc tgctttttat ataactataa aattttccta cattctatta
     5641 gaattgccca taaatacctt caagttcttc ttttaatact tcagggtcat ttataccaat
     5701 agacatggtt ctagccagta tatttgcttg gtcataagtt gatgtccatg agaatacacc
     5761 acctagacaa tttctaagaa catattgagt ttttaagaat attgataaag catcatcata
     5821 tgacattgca aaatctccat ccttaactaa atatggaact tgagcagtat tatcaaatct
     5881 atattcatat ccatttttat ttatataatc ccttctaagc tcatcataag ttcttgttat
     5941 agtagcacct aatcttccat aaaatggtat accaagaagg attttttcag aaggcattcc
     6001 agcattctca agatttctta ccattgcatc aacactatat cctggcaaag ataagtctga
     6061 atcaaaaaga tttgcttgat gttttctacc atttgggcct gtttcacctg ctgtaaaatc
     6121 ataactcata agattaaaat aatctattat aggagctatt ttatctattt cagcacttga
     6181 atttatatat cctctatctc ctgttccagc tacactaagc catttatcat ctcctataac
     6241 atctcttatg gcagttagta agagtgtaaa gttttctcta tcttgaggtc ttgatgttat
     6301 tccagatgca ctacttccag gatattccca gtctatatct attccatcta aagcatattc
     6361 atttatcatc tgattgacct gtcttgcaaa attatatcta gatgtaggtg ttaatgctgc
     6421 atcagagaat ccttcagctc cccaacctcc aatagctaca attactttta agctaggttt
     6481 ttcaccttta agagcaagta attgatttaa aaatctagga taaggaataa acaaatttcc
     6541 agactggtct atctcagcaa aagcaaatat aactgcatct aatagttgag catctacatt
     6601 agttggattt ccaagaacat attctgctac aattggattt atatctggac agtttgcatt
     6661 ttcaactatt ggattgtatg ggtctgttat ttgtggtcta actaattttt tagaatcttt
     6721 taaagttttc atagctattt ttttagtatt tttttcgcta gtataatcgt agtttttata
     6781 attatgttta tagaaatctt cataactact atcttctttg ttatagctag aatttatttt
     6841 gtcacaagat tcatctttat catagttaaa attcatttca tcacaagact tatctttaaa
     6901 cttatcttgt ttggagttgt gaactttgtt agtattttta gtatgttttt tctcatgatt
     6961 gcaatcatct ttaatatcag tcttgtgttc ttgtttacag tcaagagctt tgttcaggta
     7021 gtcatgatgt tcatgttcac agttacattc attttcaatg tttttagtat gttctttttt
     7081 ccagctacaa gtattatcaa ttttcttgct cacttcttca tcattataat tatctggtac
     7141 aaaacataag taccagtcca tacatgaata tttcttatta caactgttaa ctctattttt
     7201 taattcctta tagtttttag gataaggtaa aatcactggc attccaggaa accagtttgc
     7261 aggagtaact atattatctc tatcactagt ctgaagtgca tctactattc taagtatttc
     7321 tggaatattt cttcctgtag ttagtggata ataaagaatc gttcttagaa tttgattatt
     7381 atctataata aatacagagc gaacagttga tgtatcactc attggttttg atatcatgcc
     7441 gtataacttg gcaattctca tatctctatc ttctataata ggaaatggaa tttctacacc
     7501 tgtaagtaaa gaaatattat acatccaagc taaatgtgaa ctgttactat caacacttag
     7561 accaattagt tctgtatttc tttttttaaa ttcgtcataa tatttagcaa aacataaaaa
     7621 ttctgtagta caaactggtg taaaatcacc aggatgtgaa aataaaacaa tccaattacc
     7681 cttatagtca gagagtctaa taggaccatt tgttgtattg gctttaaaat caggagcctt
     7741 tgaccctaaa cttggcaaat ttggcatgta aatcacccct caataaatta tcatttattt
     7801 tattctatta attaatacat attagtgtta ctattttatt aaaagttaag taatccttat
     7861 gtcaaaaaaa gatatttatt aaaaagattt aatattttcc taatataaat gacaatagta
     7921 attgctattt ttttactaat ttggtatagt aatggataag gggtagtgtc aattttagtg
     7981 attatacgag gtgagtggat tgaaaaaaat tctaagaatt ttagttatct ccatgatgat
     8041 atgttttgca atgacttttg cattagtgtt aataaattca gttatgggag tacatatagg
     8101 attggattat tactttttga tgggaagtgt gcttacagtt tgtttaacaa ttggaattgt
     8161 tttatgcaat gctagaaaaa tatttgatag caaagctata cacaagacaa gaacgaaaac
     8221 tgtcagtaat aataaaaaac aaaagagtca ttctagggat ataaaaagaa agatatcata
     8281 atttatgtaa aggaaatatt tatgtataaa ataatgattg ataggtaaaa taaaaacacg
     8341 atttaacttg tgtttttatt ttttttcaag aattatgata taataaccaa taagaaattt
     8401 agtttaccat aaaatataaa gattattaaa tggagaggga actataatga attttagaga
     8461 tttatttgag aaggctgtag attttattga tgaaaaaagg aaatctatag ttgcgatttc
     8521 attatcaaca ttaggtgtaa ttgctttgat aatagtattt ttcttaagta gtaacgagtt
     8581 tagtgtagga aatgaagcta atgagttgtt aaagattata gagaaaagac agtattctat
     8641 agctgttgat tactacacaa gtatagaaaa aaagttttct gattcaaaaa tggaaaggtt
     8701 taataagtct gtttctaaaa aaataaataa attgctatta aatagtggtg ataaatattt
     8761 agatggagac ataagtcaag aaagttttat tggattaata aataccgtga aagaattaaa
     8821 caaaataagt atagatgtgg atgacttatt agctcaagct gatagagtta gagaaatgta
     8881 taaagaagaa aatactactt atgatattgc cattaactac ataagtactg tttcaatatt
     8941 aaatggtatg ggtagtaacc tagatgtata caaacagaat atagaaacaa tcaaagagtc
     9001 tagagatgtt tatgactcag cagtaaaaga ccaaaaagca tataagtatt atgaagctat
     9061 tgaaaagtat aataaagtat taaaagaaga tgaaaagtat tacagtatgg ctcaaaatgg
     9121 gaaagaacaa tgtattgaag aaatgtatga ttactatata agtagagctg aagaggcaaa
     9181 tacttctgga gattatgaaa gagcattaca atatattgag tatttaaaag acgattattc
     9241 agatgatgaa aaggtacaga gtttagaaaa aaaatataaa aagaatttat cattgtatac
     9301 tttaacatct gatgacatta taaatgtaat agctaaaaaa agtggcaaag acaaggcaaa
     9361 tctatctata aattcacttc cacaaatgat aaaaaataat aaatactatt atgctgaagt
     9421 atatgagtat gataaattaa taaatgaagt tttggtaagt gcaaaaacta aagatttata
     9481 ttcatataaa gatggtaaaa aagattacaa agtagattat ggtgatggat actttagaat
     9541 attagaagat ggaagttatc aatttggtat tacaaaagat aaagctaagt ttgtgttaac
     9601 taatactctt gatgaaaaag aaaataagta taaaaagatt gagatattag atatagagaa
     9661 agcagataga tatgtaaaaa gtaaaaagag tttagaagaa ttgtttggaa aatataaaaa
     9721 tatttattat tatgctgtag taaataaagg cttatttaga ggcaaagaag tttatgctat
     9781 taatatttac aatgaaaaaa tatatgccat atcagaaaat ggactaaatg aatattaatt
     9841 aataatatta aattaatata taattaaaaa gtttttgggg ttttaaggcg cattatgtag
     9901 taatataata atagggatat ataaataatt ttattgtaag gagaagtatg ggatgagtaa
     9961 tgtgaacaaa aagctagtta tagctattgg agctgtagca ttagtagtaa taatttttat
    10021 aggcataatg tgcatgcaat ttaaagggga gaaaatagca aaaaacacat atgttaatgg
    10081 agtaaatatt ggaaaattaa ctaaatcaca agcaaaacag gaattagcta aaaaatacaa
    10141 attagagaat gttgatttta attacaatga taaaagctgg aaagtaaaga gcaaagattt
    10201 aaatttatct actgacctgg ataaaacagt agaaaacgca tataatttaa atagaaagag
    10261 tgctttcttt ggcaatctat caaaaactat ttcagcaaat tttggcaaaa aaagcaactt
    10321 agtagttgcg ataaattatg ataaaaataa attaaaagca gaaatggaaa agatagctaa
    10381 agaaatagat gtagatgtaa aagatgctac acttgatata agtggtgaaa aagtcaaggt
    10441 tataccagat tctgatggtc ttaaaatgga tatttctaag agtatggaga attttgataa
    10501 tcaaactaaa aaaggtaact ataaaaatga gttagtagta aaagctactc cagcaaaagt
    10561 aaaaaaagag caacttgcta atatagatac aaatttaggt acgtattcaa caacatttaa
    10621 aacttctcaa ataaatagaa gtataaacat aaaattagca acagataata taagtaatgt
    10681 attacttatg ccaggagaaa ctttctcatt taataaacat acagggaaaa gaagtaagga
    10741 aaatggatat aaaagtgcac ctgtaataat ggaaggtgag atggaagaag attatggtgg
    10801 aggagtttgt caagtttcat ctacgttata caattctgtg ctatacgcag gtttagaaat
    10861 tgttaatgta aaaaatcata ctataccatc aagttatgtt ccaaaaggaa gagatgcaac
    10921 tgttgcagat agtggaatag atttcttatt taaaaataat ctaaagcatc cagtttacat
    10981 aaaaaattac gtttcaggaa atcaaattgt ttgcaatata tatggaagtg cagaagataa
    11041 acaaaatata acaatatcaa caaaattaga tggcgtttca caaactacaa tgaaaagagt
    11101 taatgaccct acaatgccta aaggtaaaga aaaagtagac aaaagtggaa gaaatgcata
    11161 ttctgtatca acatatagaa catttaatga tgcaaatggt aaaaaaatta aaactgaaaa
    11221 aatagctaat tcatattatc ctaagaagga aggtattata ttagttggga ctatggaacc
    11281 aaaaccagag gaaaaaccaa atacagacga aaataagaat aatcaaaata caaacaatca
    11341 aaacccaaat aatcaacaaa aaccagaaac accaccaact gataataagc ctaatgagac
    11401 acaaccacaa ccgcaagctt aaagttctca agaataggat ttatgtagag taataaaaaa
    11461 tataaatcta aataggaggg attactttga atttaaataa tataacgaat tacgttcaaa
    11521 gtatttgcga gaatatttct aatgtcttgg atgtagatgt tactttagtt acaaaagatt
    11581 taactaggat agcaggtaca ggaatattca gaggtaaaat aggagagaca atatcagaga
    11641 agtcagtgta ctctcttgtt ttaaaagatg ggaaatccta tgttataaat aaagaaatag
    11701 aagagaactg taataaatgt gcaaatagag aaaattgtaa agagttagct gatatatgta
    11761 ctcccgttaa aatgggagag catatacttg gaatcttggg tatagctgcg tttaatgaag
    11821 aacaaaaaga aaagatactt tcaaaagata aggagttaac tgcttttgta aggagtatgt
    11881 ctgatttaat atcttttaaa ctagatgaac tttataaaca agaagttaag actatagatg
    11941 accttgagag ggaagctata gaaaatgcca taaagaaata tggaagtaat acagaaggta
    12001 tgaaaaaagt agctgaggca ttaaatatag gtatagctac actctataga aaagtaaaaa
    12061 aattcaacat taaaagttaa tagtattgaa aaagaggtaa cctttttatg aaaagaagag
    12121 gattaccttt tttattatga gaatggtgga aaaatatgta tagttttgac ttgacatatt
    12181 aaatatgtta tcttaaatat atattttaga gtggaggtga gtattatttt agttaaagat
    12241 ataaaacaag acatagaaag catgcctaga aaaattttag atatatcttt gatagaagat
    12301 aataaaagtg atttgacttt ttttaatata gaagaagaag acggtgatga aacattaaaa
    12361 gcattaaaac agagttatgt gactacaaat tataaagaag aaactctatt aaacttgcaa
    12421 ggagctttaa attcaaagtc agatacaacg tctaaggagt taaaattaga atcatataaa
    12481 agatataaca atgcattgga tttagcatat aaaaattata taactagtgc cattaatatg
    12541 gttaataaag ctctggagat aaatcctaaa gacattgata tactaaatct tagaggtctt
    12601 ttaaaattat taaaatgtga ttttgctaaa tcatttgaga gtttttatac tgctttatgt
    12661 tatgaaaata atcatatatc tagaaaatat gtaaatctaa tttcatctga agattttaaa
    12721 acttttttag gaagatacaa tcattctata agatttataa atgaagaatt aaattatgag
    12781 tcaatacata ttttagagaa cataattgaa gaagaacctg aactaataga gccatatgta
    12841 attttatctt tgttatatga taaactaggt aatgtaaaaa agagagaagg atatttagac
    12901 aaacttaaag agctagataa agataatcct gtatttgaaa aaaatgaaga agaaaaagaa
    12961 gatacaagta aaaaggaaga aataaaaaaa gtaaaaagaa agaaaagtat tttgccatat
    13021 attatagttg gctgtgtttt aataggtatg gggatatatt tgattcaaag caaaaagaga
    13081 atagaaaact tgaataacca aataagtagt aaagaggaaa agttaagtga aactgataaa
    13141 aagttaggag aaacgagtaa aaaattagaa aaaacgaata aggaattaaa tgaggttaaa
    13201 aataaagaac cagaaagaga gataactgta gctgatgagg aagatttata ttatcaagct
    13261 ctaaatttaa agaaaaacaa agaatatgaa aaagccatag ataattttaa aagtgtagtt
    13321 tcttctggta aaactaaaaa atatatttct gagtcaatat atcaattagc aataacaaat
    13381 aaattattag gaaataagga tgaagctata aaatattaca aaaaatatat aaatacttat
    13441 actaaaaatg accagtatta tgatgattca tattacgagt tgggtatgtt ttattatgat
    13501 aatggcgatt taaaaaatgc acaacagact ttttatagtt tgagaagtga agttccagac
    13561 agtatgtata acaattcaaa aataaaagaa attttaagtg aaaaataggt ttatatagtt
    13621 aagttttttg aaacaaccaa gtatgtattt tattaaatta aaataaaaat aaatttaaaa
    13681 agagattgtg tcaaattata actttctatt ttaaagaaag atatttgctt gttgataaaa
    13741 ctacaaatat atgatttgaa gcaatctctt taatttttgg aggtggaaaa tgaaagtttt
    13801 caatcaattg gtaataattt tatctatctg ggtaatagga gagtatataa gttcctttat
    13861 acaaggaatt atacttattc ctgggagtat agttggtatg ctattattat ttctactact
    13921 acaatttaaa gttttagact tgagtagtat agaaaatgta agtggatttt tcctcgataa
    13981 tatggctata ttttttattc cagcaggtgt atcacttata aagtcattag acctaataag
    14041 ggaaaatgta ttagtgcttt tattagtcat atgtttaagt acactaatag taatgtatac
    14101 tactggaatt attgtggaga aaatgataaa aaagaaggaa gaaggacaaa aaaatgtata
    14161 atgtattaca gacaccagta tttggtataa tagtaacaat agtttttttc aatttaggaa
    14221 gatacattca acgaaaaaca agtaatccta tatgtaaccc tttattaatt gcaatcgtag
    14281 gaataatatt gtttttaagc atatctaaaa tcccttatga gaattacaag ataggagctg
    14341 atagtctaaa tttcttttta ggtcctgtca cagtagtact tgcagttcca ctttataaac
    14401 aatttgaatt atttaaaaag catatgtttg agattattgt aggaataggt tgtggtatag
    14461 tgatttcttt tgtatctgta ttgataattg gcaaaatagc taattcagat gtaagtataa
    14521 taaattcact aattccaaaa tctataacaa caccaatggg aatatcttta gcaaattctt
    14581 taaatggtgt agaatcaata acagttgtag ccataatttt tacaggtatt tttggtggta
    14641 tggttgcttc tacagtattc aaactgggga atataaatca tccagttgct aaaggaattg
    14701 ctcttggaac atctgcacat gctttaggaa ctacaaaggc ttttgaactt ggagaaatag
    14761 aaggagctat gagtggtgtt tctattggag tctctggtac aattacagta atactaattc
    14821 caattataat gaattttata taaaaaataa aattaagatg agtaccttga atacttaaaa
    14881 caaaataaaa taaaaaaaat ttaaaataaa aactgaacga aaaaaatgca aataatgaaa
    14941 taaaaaaatc atgtactgaa attataaaaa gaaaaatttc atttaggaat taattataaa
    15001 gagacaatta ctaaacttga atttgctaaa ataaaataaa ttactgctaa aatagatatt
    15061 tagaaaaaaa tactataaat ttactatatt gcaagttaaa taataagcag tatcatatta
    15121 tgctagaaaa agtttcattt aaggcataaa tgagatgatt tgaacgtttt taaagattag
    15181 aatataaaag attgcactag acatatatga aaaagataat ataatagatg atgtaataga
    15241 tgacagttca agaaattata aaaaaaattt atttgaaata ttatctatta catcaggaat
    15301 aatatatgta agagtaagct tttaaaattc taaagattta gaaagattga agggagaaag
    15361 aaatgagtga agttaagaat accctacaaa aacatccagt tttaagatat tcaacaatgc
    15421 tatttgggat gttgatgaca tctataggaa taaatgggtt cttaagacca gcacatctgc
    15481 ttagtggagg tgctacaggt atagcaacat ctataaacta tttgacaaat ataaatgtag
    15541 gtttattaac atttttaatc aatattccaa tatttatctt agggttcata taccttgaaa
    15601 aagagttttg tatttcaagt ttggtaaata tgattgtatt ttcattatta ttaggagcaa
    15661 ctcaggaaat aagtaatatt atacctatac atgatatatt gcttcaaagt gtatatggtg
    15721 gtatattatc tggtttagga gtcggagttg tatttagaac taggtcttct caaggtggta
    15781 cagatataat agctgcaata ctaaaaatta agaagaatat agaaatgaaa gacactgctt
    15841 tagcaataaa tggcttgata gtacttacag gaagtttttt atttgggttg gatttagcat
    15901 tatacactct tataggtcta tttctaaatg cgtattcaat gagttttatt aaagatgcaa
    15961 tgaattacca aaaatctgtt atggtaatgt caaatgaagt cgatttaata gcagaagata
    16021 taatgaagag tttagttaga ggtgtaactt ttttagatgc agaaggtgca tacactcatc
    16081 aaaaaaagaa gattatatat acaatagttt catcaaatga gataccaaag ataaaagata
    16141 tagctttgaa gtatgataaa aaggccttta tatctgtaaa tgatgttaca gaagtaaagg
    16201 gaagagggtt taaagcaaaa gatttataga aaattttaca agctgggagt ttataatgta
    16261 aatttgtata ttatatctct cagcttattt aaatttagta tacgaaaata aaggttttat
    16321 taatgatttg tgtatcactt taaaagatat cattttaaaa ttaaaattga aaaaattact
    16381 taatatgtag aaaacatgac cactattagg atataataaa tttaatagat ttagtaaaat
    16441 acctaggaat aacgttggag gaatggcatt atgaatttat ctaaagaata tgttaatttg
    16501 tatgataatt atttgaaata tataaatgaa gtaaacaaag agattagaag tataaagaac
    16561 atgaagaaac gtgttttaaa aaggggcgat tttgctccgg aaaatgaagt attagaattg
    16621 gaaggaaaaa caatacgcga tatagatgat gtcgatacaa aaaaaccaaa aaataaaatc
    16681 tataaatttt caaatgggga tgtatatgta gggaaattct tagagggaaa gatggagggg
    16741 aaaggctcat atactttttt tgtagatgaa ggaacagtta tggagtacat aggagaattt
    16801 aaagaagata aaaaaaatgg aaaaggaaac tttatatttt caaatggaaa tgaatatata
    16861 ggttattttg aggaagatat gatgagcggt attggaaata tgttatatag tagtaaagat
    16921 gaatatattg gaacttggaa aaacggaaaa aaagatggaa aaggtattta taagtggagt
    16981 gatggatgta tatatgcagg tgaatttaaa ggtggaaaga tggaagggta cggtatttgc
    17041 tataatagta aaggggaagt actttatgaa ggagaatgga agaataattt gattcatggt
    17101 aagggaactt atatatggga aaaaggtaaa aagtatatag gtgaatttat gcatggtaaa
    17161 aaacatgggc aaggaacttt ttatctaaat aatgaattgg tttatgaagg gacttggaaa
    17221 tttgataagc catctatatt tgatagaaca ttagatgaaa tattttcctt gagactgtag
    17281 ctttatctaa ttagtagata aagctttttt ctaatactta ctgtaaatag gtatgaatga
    17341 tattgattag tatacactat caatattaac ttaataaatg caatattaaa aaattttgat
    17401 tgattttaag aaggagagat atattgaaga gattaattgc tttagtgtta gtagtactta
    17461 ttgttttatt ttatagtcct gaattattaa aagaaggata tgatttaaaa gaaaagtttt
    17521 ttcaaagtga aatatggaat gaatttttag acaaaataaa tataaatgat aattctaaga
    17581 aagataaaaa agaaaaaagt caaaatttac aatcaaataa tactgaaggg gagaacttag
    17641 gagagtcaga tataaagaat tttgaaaaaa taagcttagg agataattta agctatgttt
    17701 tatcaagtat cggcaaacct ggtagaattg atattagtga atatggtttt gattggtatg
    17761 tatataatca atatggaaaa gaatttgcaa tggttggact tgaaaatgat gaagtagtag
    17821 cactttatag taattctatt aattcatgtg aaaatcaaga tataaaacta aatcaagata
    17881 gacaaacagt gagaactaag attacaccat taaaatataa gagaaaagga aataccagat
    17941 atataataaa ctcagaaaat caatatgata taataagtaa ggaaggtaag tatattacaa
    18001 ttttctacga tattcatgaa gaaaatagag tttgttcata tttaataatt gataaaagca
    18061 ctgaagatga gtttaaaaat ttatatccag atgatagcga agaacttaaa aaatgttttg
    18121 aacttgaagt tatagacctt gtaaatagtg ttagaaatca aagagggtta aactcattga
    18181 gatacagtga acaggcaact ttgagttcaa gaaaacatag tgaagatatg agagataata
    18241 atttttttga ccatgtaaat aagaagaatg aaactccatt tgacagaatg aaaagagaag
    18301 gtatagtata tacttcagct ggagaaaaca tagcagcagg acagattaat gcaatatatg
    18361 cgcatgaagc ttggatgaac tctgaaggac atagaaaaaa tattttgggg aactataata
    18421 atattggagt tggagtaata tttggaggaa gctataaaac atattacact caaaattttt
    18481 ataaataact aaaatatttg aaaggaagtt aaaaaatgac atcatggaaa aaaaagacag
    18541 tatataagtg cttaatagca gtagccttgt tttgtggaat agtgttaata tctaatttta
    18601 gtaaagtttc tgctttgatg atggatacta atggaaatgt tttgattaaa catggttcaa
    18661 gggaaaaaaa gctaatagca attacctttg atgatggtcc tcatcctaaa gaaactagtc
    18721 aagttttaga tgtattaaag aaatacaatg taaaagctac tttttttata gctggaaaac
    18781 atgcaaaatg gtataaagaa ccattagtaa gagcaagtaa agaaggtcac gagataggaa
    18841 atcatacttt caatcatcct gatataagta atttaagttc tagtcagata gaagaggaga
    18901 ttgttaagtg tgaagacatt ttgaaggaag taactgggaa aaaacctaca ttatttagac
    18961 caccattcgg aagttataga gaaaaagact taatagaaat tgcaaaaaag catgactata
    19021 aagttgtttt atggacaggt gtagatgtga aagattggaa aaatccagga gctaatagca
    19081 ttgcagataa aataattaat aaagtacaaa atggagatat aatacttttg catgattatg
    19141 caactaatga tacagttgaa gctttagata tgtttattcc aaaaatgatt gaaaaaggtt
    19201 ttaaatttgt aacagtttct gagttgataa aataattttt aggggggaga tttacagtat
    19261 gattcttatg atagacaatt atgattcttt tgtatacaac ctagtccaat atatagaaga
    19321 attaggggaa gcagttgtag taaaaagaaa taatgaaatc aaaataagtg acattgaaga
    19381 actaaatcca gaggtaattg tgttatctcc aggaccgtgt tctccaaaag aagctggtat
    19441 ttgcatagat attgttgagc attttaaggg gaaaaaacct atactaggaa tttgtttagg
    19501 tcaccaaact attggacatg tttttggagg agatattata aaagcacaac agcctgtgca
    19561 tggaaaagta tatagtatca accatactaa taaaggagtt tttaggggac ttaaaaatcc
    19621 tttaaatgtc actaggtatc attcactaat agttgactcc aatacagttc caaaagaatt
    19681 agaaattaca gcaattactg ataaagggga aataatggga attaggcata agaaatattt
    19741 aatagaagga gtacagtttc atccagaagc tattttgtca gagtatggtc atgaaatgct
    19801 taaaaatttt ataacagaag ctagagaaag ggtacatatg taatatgata agagagataa
    19861 atactaaatt aaattctttt gagatattta ctatttttag aaatgaacat gacagtttta
    19921 tattagatag tgctatggat aaagaaaaat tgggaagata ttcttttata agtagccaac
    19981 catttaaggt attaaagtat aaagatactg atgaaaatcc tttagaagtt ttaaaagaag
    20041 aactacataa gtatagggtt gtaaatgata ctaatcttcc atttgtagga ggtgctgtgg
    20101 gatatttatc ttatgattta ggtaattaca tagaaaattt acctagaact gctgtagatg
    20161 atattgagat gcctgatatg tattttggat tttataatca tgtaatagtt atagaccatc
    20221 ttgtgcaaaa aacttatata gctactccaa acatagatat cgaattagaa gaaaagataa
    20281 tagatgatat agaacaaaga atattaaaag aagagaaaaa aggtatagat agcatatgtt
    20341 atgaagaaaa agaagtaaca cctataagac ttaaatctaa ttttacaaag gaagaattta
    20401 aaaatgcagt tcaaagtgtt agagagtaca taagacaagg agatatatat caagctaatt
    20461 taacacaaag gttcagtgga gaaacagaac ttacaagctt tgaattgtat agggacttaa
    20521 gaagatttag cccagcacca tttggggcat tcttaaactt tgaagatgca catatattat
    20581 caaattctcc agaaagattt ataagatgtg ttaataagag aatagaaact agaccaataa
    20641 aaggtactcg tccaagaggg aaagataaag aagaagattt aagacttcaa caagaactta
    20701 gaaatagtga aaaagataga gctgaactac ttatgatagt agatttagaa agaaatgata
    20761 ttgggagaat atctaaaact ggaagtgtta aagttccaga gctatttgta attgaaccat
    20821 atgcaaatgt aaatcactta gtttccacag ttgtaggtga gttaaaagat gataaagatg
    20881 ctactgatgt aataaaagca acttttccag gggggtctat aacaggagca cctaagatta
    20941 gagcaatgga aattatagac gaattagagc caacgcaaag aaatgtatat actggttcaa
    21001 taggttatat aggttttaat ggtgatatgg attttaatat agcaattaga acaataatca
    21061 aaaatgacaa aaaagtctac tttcaagttg gaggaggaat gacttgggat tctgacccag
    21121 atgaagaata tcaagaaaca cttgataaag ctaaatccat aatgaaggct ttgaggggat
    21181 attatgaaga ataatgtagg ttttgatagt agtttaagta aatttggaat tggtttgttt
    21241 gaaactatta gagttaaaaa tggaatagca gtagatttag atatccatat agaaagaatg
    21301 ctaagctcaa taaattgttt ggatttagat ataaactata aaaaagattt cttgataaat
    21361 gaaattgtta catatataaa aaaagaaaat gtcattaata aagcacttag aattactgtt
    21421 tttgatgaag gttataatat atctattagg gatattccat ataatcaaga gacgtatgag
    21481 aaggggttta aattagctat atctccaata gttaggggga atagtttaat tcatagacat
    21541 aagactacta attattttga aaacatatat accaaaaata tcgcaaataa gactggttat
    21601 aatgatggta tatttttaaa ttcagaaggt gtaatattag aatgttctat gagcaatatt
    21661 ttctttataa aaggagaaag agtgtacact cctagtgata gattaccgat attaaatgga
    21721 ataataaaaa aaaggattat agagatttgt gacgaattac atatagagtt gatagaaaat
    21781 gaaattaata tatcagaaat tagtagtttt gattttgttt ttgtcacaaa tagtcttatg
    21841 ggcgctataa aggttacaga aattgacaaa ataaaattta ataaagaaaa tgttgttttt
    21901 gataaaatag tgaagttatt agaataaatt aaaatattaa caatttgttg gagacaaaag
    21961 atgataggtt gtatttatga agtatacaat gaagagatga ctaaaataat agaaaaccaa
    22021 aacactttgg ctgtatattt agtgggttct agtaaagatg tagattttac tctttatgat
    22081 gtagtgataa acgatattga tgtgttcgtt tttgtaaaag aaggggaaaa acaagagaga
    22141 atactgttta aaaaaaaggg aatagaattt gatgtaaatt acttttcaaa agatggtgta
    22201 aaaaaattat tatcaaatag ggaatatttc tttgtgaaag aaatgaaaga tgctaaagta
    22261 ctgtttgata gggagaatat atcacatatt ataaaagata taagtagaga tatttactta
    22321 gaaggaccta gtaaaatgtc attagaggaa aagtctttta taaagcaaga tattggagca
    22381 aaaatattga atttaaaaaa taaagaaaaa tttgatgttt ttgaatatca ttttttgacg
    22441 aatttatatt taaaagatat aataattggg tactttaata taaatgataa atgggtacca
    22501 aaagacaaaa aattgctaaa agttttaaaa aaagaaaata atgagctttt tgaactagtg
    22561 tcaaaagtaa gtgaaaatta tgactatcaa agacttttag atgtttataa ttatattttt
    22621 aaagaaattg agacaaaaga ggttataaaa ctaatcttct agtcacaaat caatgggggg
    22681 agtttaaatg aataaagttg ataaagaaaa gatacaacat gcagtaagag aaatactaga
    22741 ggcaattggg gaagacccag atagagaagg tctaatcgaa actccaaata gagttgctag
    22801 gatgtatgaa gagatattct ctggtcttag tgaagaacca agagaccatt taaaagttct
    22861 ttttgcagat gaaaaacatg aagagttagt attggtaaaa gatattccat tttattcatg
    22921 ttgtgagcat catctagttc ctttctttgg aaaagctcat attgcttatt taccaaaagg
    22981 aggaagactt acagggcttt ctaaacttgc tagagtaata gatacattgg caaaaagacc
    23041 acaattacaa gaaagaatca ctaaaaatgc tgcagatata ataatggaag aattacaacc
    23101 ttatggtgtc ttagtagtag tagaagctga acatatgtgc atgacaatga gaggcgtaaa
    23161 aaaaccaggt tcaaaaactg ttacatcagc agtgagagga atatttgaaa aggacattgc
    23221 gtctagagcc gaggctatga gtttgattac tatgaagtag taatttatta tagatataat
    23281 catttattga gataaaattt aatttaaggg agtgtcatta attgtgtttg attatggtaa
    23341 aagaacttat ataatgggaa tattaaatgt aactccagat agtttttctg atggaggaga
    23401 ttttaataac ttggacatag caatacaaca tgcaaaagat atggttgacc aaggtgcaga
    23461 tataatagac ctaggaggag agtctacacg cccaggtcat agctatgtag actcagacga
    23521 agagctaaga agagtaatcc ctgtaataaa aaaacttaaa caggaattgg atataccaat
    23581 atcaatagac acttataagg cagatgttgc agaagaagca ttaaaattag gtgtcactat
    23641 ggtaaatgat gtttggggtc ttagaaaaga taaaaatatg gcttctgtaa taggaaaata
    23701 tgatgcagaa gtgtgtataa tgcataatca agatgggaca aactatgaca aagatataat
    23761 ggagtcaata aaagactttt ttaaggtaag tatagaaatg gcaatgagtt gtggtgtaaa
    23821 aaaagaaaaa atagtacttg acccaggtgt aggatttggc aaggattttg aacaaaatat
    23881 tgaagtattg agaagattaa atgaattaaa agatttaggg tatcctattt tgcttgggac
    23941 atccagaaaa tcagtaatag gaaaagtact tccagttgag cctaaaaaaa gattagaagg
    24001 tacaatagct actactgttc ttggtataag agatggcgta gatatagtta gagtacatga
    24061 tgtttatgaa aatttaatgg cagctagaat gactgatgca atatatagaa agtaggtata
    24121 atataatgga taaaatatta cttagtaatc taggattcta tggatatcat ggtgttctta
    24181 aagaagaaaa ttttctaggt cagaaattct ttgtcgacat ggaactttat attgattcaa
    24241 gagaagcagg tcttagtgac gatataaata aatctgtaag ctatgccgag gtttacaatg
    24301 ttgttaaaga tattactgaa aataaacaat ttaatctttt ggaggcttta gcggaaaata
    24361 ttgctgaaga agtcttgaat aaatttatac ttataaatgg agttatggtc agagttagaa
    24421 agcctgaggc tcctgtaaat ggtatttatg actattttgg tgttgagata aggagaacaa
    24481 gagatgaata aggcttattt gggaataggt actaacatgg gtgatagatt tgataattta
    24541 tcaagagcat gtgaactttt gaaaaatagt gattctatat ataaagtaaa agaatcaagc
    24601 ttatacgaaa caaaaccatg gggatataca gaacaagcag attttctaaa tatgtgtgta
    24661 gaaatagaaa cagagtttga accttatgag ttgttagaat attgtcaaga aatagagaga
    24721 gaactacaca gagagagaat agttcattgg ggaccaagaa ctatagatgt agacgtacta
    24781 ttttttaatg atgtagtttc cactgatgaa agactaacaa tacctcatcc aaggattcaa
    24841 gatagagctt tcgttttgat accacttatg gatttaaatg aagaacttat aataaatgaa
    24901 aaaacaataa aagaacattt aaacctccta tctgctgaag aaagagaaga agtaaaggag
    24961 cttgtaggtt atgaaagaaa acctatttaa taataattca aagatagaga taaatacaaa
    25021 taacaaaaag ataactgtaa aaaaagataa acttataata gctggaccat gtgcaataga
    25081 gagttatgag caacttttgg agactgctaa atttgtaaaa agtcagggag caaatatttt
    25141 aagaggtgga gcatacaagc caagaacatc tccaaattca tttcaaggat taaaaaaaga
    25201 aggtcttgaa atattaaaag cggttaaaga cgaagttggt atggctgtta ttacagaact
    25261 tatggatgta agagatatgg acgaactgta tagtataagt gatataattc aaattgggtc
    25321 aagaaatatg caaaacttta ctcttttaag tgaagtggga aaacaaaata aacctgttat
    25381 gttaaaaaga ggaatagctt ccacaattac tgaatggata ggtgcttctg aatatatagc
    25441 aatagaagga aatagtaata ttataatgtg tgagagaggt ataagaacgt ataatgatta
    25501 tactagaaat acattagatt tagctgcagt acccattatt caaaaggaaa caggtcttcc
    25561 tgttgtggta gacccaagcc atgccactgg agttagatat ttggtaaaac ctatgtcact
    25621 ggcatctttt gcatgtggag cagatggaat aatggtagaa gtacatcctg acccagaaaa
    25681 tgctttgtca gatggtgctc aatctctttg ctttaatgaa tttgaagatt taatgaaatc
    25741 aattaataat tattaaaaga ggcaagagcc tcttttttat tcgtaatcgt tgctaaggta
    25801 aatttataag ttataatttg atagttagat tatttaatat aaataaagat ttaatattga
    25861 tagaattaat ataaaaaata aaaatttttt gattaataag aaggatatca ataaaaaaag
    25921 tcgaattgtg tatattgagt gattgacaaa tttagaatat agtgtagaat agaaatgtta
    25981 acacaaaaaa ttacaccaga aggtgataat atgaatgata taaaagacat tattgaagaa
    26041 ataaaagcta gatgcgatat agcaagtata atatctgaat atatgaatat aaaacaatct
    26101 ggagctaatt acaaaggatt atgtccattt catggcgaaa agacaccttc tttttatata
    26161 aatacgtcaa aacagattta taagtgtttt ggttgtggtg aaggtggaga cgtaataaat
    26221 tttgtcatga aaatggaaaa tttggatttt atggatgctg taaaaatttt agcaaataaa
    26281 tgcggtatag aaattaacac taacatgaat gaagaaacta ggattaaaat agaaaaatct
    26341 aagaaatttc aagatattca tacggaagca gctagatttt atctttcaaa cttattggga
    26401 agcaaaaatc ttgggtatga atatttaaga ataagaggac tagatgataa aattattaag
    26461 aaatttggtt taggattttc tttggattca tggaattcac ttatgaatac tctaatcagt
    26521 aaaggatata aaaaacaaga cttattggag tgcggtctaa ttgcaaaaaa tagagatgga
    26581 actaattgct atgataaatt tagaaataga gttatgtttc ctatattcga ttatagagga
    26641 aatataatag gatttggggg aagagtatta gacgattctt tgccaaaata cttaaattct
    26701 ccagacacgt taatttttaa taaaaaacag aacttatacg gattaaattt tgctagaaaa
    26761 aatttagaaa gtaaaactat agtcttagtt gaaggatata tggatttaat ttcgctttat
    26821 cagtatggta taaaaaatgt agtagcaacc cttggaacag ccttgactga acaacaaggc
    26881 attttaataa agagatatgc ggacactgct attatatctt atgattctga tgaagcagga
    26941 ataaaagcga ctttaagagc tatagatata cttactaaat taggtatcaa tgtaaaagtg
    27001 ttagacttaa aagatgctaa agacccagat gaatttgtaa gaaaatacgg acttagtgat
    27061 tataaaaaag ctatggatgt ttctactcac tacataaaat ataaaataga tcatcttaaa
    27121 aaagaattta acattcaaaa agatgaagaa cgagtgaagt tcgcaaaaga agcttctaaa
    27181 attataaaac aattgacaag tcctgttgaa attgattttt atacaaagta tttaagcaat
    27241 caaatagata ttaatgtcga gtctataaag agagaagttt atggtaaaaa ttacaataag
    27301 ccatataata acaaaaatca aaagaaaata gaagaaaaag taattgaaaa agtcgaagtc
    27361 agacaagacg gaaaacaatt agttgaagaa actctaataa aaataatgtt agaagacaaa
    27421 aaaattagag aaatagcgtt acttaaagta gaagaaagtg attttttatt gaaagaaagt
    27481 aaagaaattt taaattacat gattaaaaat caagaattgg acaaaataac tattgacaaa
    27541 ttgaaaagtt taaacatatc cgaagaatac ttaaaagaat taaattcaat ttctttgaat
    27601 agtataaact tagagaacac aaaagaaata gaaggaataa taacaaatat cagaaaaaac
    27661 tcattagaag agcaaattaa tagcttgttg agagaacaac aagaattaga aaataataat
    27721 gatatgaaag aggtagatgg tagagttatg gaaattgctt taaaaatagt tgagataaat
    27781 aaaattttaa agagcttgta gagaaaggag gtttgagtgt ggaaaataaa tcgaataaaa
    27841 aagagttaaa aaaggttact gctaagacat taatagaaaa aggaaaaaag caaggttctc
    27901 tgacacttgc agagataatg gaagcttttt cggagactga acttgataag gatcaagtag
    27961 aaaatcttta tgagacttta ggaaatttgg gaatagaaat aacagaaaca aaaaactata
    28021 aagctgatat agatttttcg gttgctgatg acgatttaag tataggccac ttagatgaag
    28081 atgcagaggc aatttcacat gatgactctt ctgcaataga aatagaaact gtggatttat
    28141 ctttaccaaa agggataagt atagatgacc ctgtaagaat gtacttaaaa gaaataggaa
    28201 aaattcctct acttaaacca catgaagaag tagaatttgc tagaaggatg cacgaaggtg
    28261 atgagatagc aaaacaaagg ttagttgaag caaacttaag actagttgta agtatagcaa
    28321 aaagatatgt aggaagaggt atgcttttct tggatttaat acaagaggga aatttaggtc
    28381 ttataaaagc agtcgaaaaa tttgactata caaaaggata taagtttagt acttatgcaa
    28441 catggtggat aagacaagct ataacacgtg ctattgcaga ccaagctaga actataagaa
    28501 taccagttca tatggtagag actataaata agctaataag agtatcaaga caactacttc
    28561 aagaacttgg aagagatcca aaaccagaag aaattgcaaa agaaatggaa atgacagaag
    28621 ataaagtaag agaaataatg aaaatagctc aagaccctgt gtctttagaa acaccaatag
    28681 gagaagaaga agatagccat ttaggtgact ttattccaga tgatgatgct ccagccccag
    28741 cagaggcagc agcatattct ttattaaaag aacaaataga agatgtactt ggttcattaa
    28801 atgatagaga acaaaaagta ttaaagctta gatttggtct tgaggatggt agagccagaa
    28861 ctcttgagga agttggaaaa gagtttgatg taactagaga gagaataaga cagatagaag
    28921 caaaagcact aagaaaacta agacatccaa gtagatcaaa aaaacttaga gattatttag
    28981 actaaaaaga tacatcattg atgtttttgt gataaatgcc atgagataag tgatatctca
    29041 tggctatttt gtagtaaaga tttaatttta tttttgtagt tatttcagtc taatatctat
    29101 aacttggatt aaatcctata aataaaggga attgaaagga gaattatatt tgaaattaac
    29161 agatagatta ttgaaaatag catcattagt gtcagatggt aaaaaaatag ctgatatagg
    29221 gacggaccac ggatacatac cagtatattt gttaaaagag ggaaaagtac catttgcagt
    29281 gctagcggat gttaataaag gacctcttga caatgcacat aaagaagtca tacagaacaa
    29341 tcttttagat aaagttgatt tacgattggg ttctggtata gagatacttg aaatagggga
    29401 agtagaagaa gtgataattg ctggtatggg tggaatcttg ataagtgagc ttctcgaggc
    29461 taaaaaagaa gtagcacata atgtagaaaa attaatatta cagccaatgc aagcacaaga
    29521 agaacttaga tattaccttt taaacaacgg atatgaaatt ttagaagaag ttttagtgag
    29581 agaagatttt agaatatatg agataattgt tgccaaatat acaggtaaaa atactataat
    29641 agaagatgag atatattatg aggttggaat aaagctttta gaaaacaaag attctctttt
    29701 taatgatttt atagaaaaaa agataaagac atatagttct atagttaata aattagaagg
    29761 gaaaaatggg gaagctatag ataagaagag aaaggaaagc gaagttgcaa taaaaaaact
    29821 tgaaaattta ataaaataaa taggaggtat atatgctatt aaagagtcta actagaaaaa
    29881 ttgaaaaaaa gtatcctcta aacttagcag aggattggga taatgtaggt cttatagttg
    29941 gagattttga tatggatgta aaaaaggtat tggtatcttt agaagctaat gaagatgtta
    30001 ttgatgaagc tatatccaaa aatatagatt taattgtgac acatcatcct tttatatttg
    30061 ggaaaattaa taaaattaat agtggagatt taaaaggcag acttatccaa aaacttatta
    30121 aaaatgatat atcactttat tctatgcata cgaattttga tatagcattt gatggtctaa
    30181 atgactattt tatggaaatt atggaatttg gtaattcaaa ggttttagat ataactaaaa
    30241 gcgaaaattt gtataaatta gcagtatacg ttccacataa ctattcagat gaacttagaa
    30301 aggttttaag taattcagga gcaggtcata tagggaatta tagtgattgt acgttttcaa
    30361 tagaaggtga aggacaattt aagccattag aaggctctaa tccgtttctt ggaagtgtaa
    30421 atgatataga aactgtaaat gaagttaaaa ttgaaactgt agtaccacag aagcttttag
    30481 gtggggtaat aagttctatg ttagatgcac atccttatga agaagttgca tatgatttgt
    30541 acaagttaga aaataaaggt gagatatttg gactaggtag aataagtaaa cttgataaaa
    30601 gtatgacatt agaaagtctt tcaaggaaaa taaaagagaa attaaatatg aaacacataa
    30661 gagtagttgg aaaccttagt acagacataa cgaaagtagc cgttgtaaca ggagctggtt
    30721 cagaatttgt aaaaaaagct aagagacaag gtgcagaagt actaattacg ggtgatgtaa
    30781 agtatcatga ggcacaagat gcacttgata ttggaatgtg tatagtggat tgtggtcatt
    30841 ttgatacaga agatatattc aaaaatgtta tgaagagatt tttagatgaa tttagtgaaa
    30901 ttgaagtaat aaaaagtaat gtatatttga atccatttag tactatttag cagtgtttga
    30961 taataaaaat actatataaa aaataaattt gagtttaatt agggggataa tgatgaaagt
    31021 agcagtaatt tttcatagtg tttgtgggag tacatattta ttagctagag aatataaaga
    31081 agcgcttgaa gaaatgaata tcgaggtaga tatatttaga gtaagtgacg aagttgcaaa
    31141 aacacttcct caatattatt tgataaattc caaagaatac aaagacgaat ttgaaagtat
    31201 aaatgtaatt aaatctggaa aagaaatatt agattatgat gctatattta tgggttcacc
    31261 aacatattat ggaaatgtat ctggaccaat gaagatgttt atggatagtt tttctgatat
    31321 ttgggtaggt actcctttga gtggtaaaat atttggttgt tttgctacag caggttcaca
    31381 acatggagga ggagaattag ctttgcaagc tatgaatata tttgcccaac atatgggaat
    31441 gactcttttg tcagttccat gttcagtaag aggaggttat ccagcgtatg gaatacttca
    31501 tatagcagga gataattctg atataagacc aaatgatgat gccaagatag gtataagaga
    31561 ttacctaaaa agacttaaca tataagaata tcatataaaa atacctaaaa gcgactgtat
    31621 atacttttag gtatttttac ttaattataa acgtgaagct ttaccagaaa tatggtcaat
    31681 ttctatttta acaacagttg ttaagttcat atccttctct atgtacttca tgccttcttc
    31741 aaagaatcct ttagaatatt tttttattat ttctacaaga gcatttttct tttcttcatt
    31801 ttctacagtg caagcttttc caaaaactat agcactagaa taagtagtac taaatttatc
    31861 tggaataaca ctttcattag caactactaa aaaagaaact ttgttgtttt tcgatatatt
    31921 atctaactta tgcccatttc ttgcacaatg aaagtaaata gaatcattaa agtatacata
    31981 atttacagca actccataag gataaccatt ttcagatata gtggaaaaag taccaaattc
    32041 accatttttt aatacttcaa cagtatcttc ttttgtcatt tctctttttt tcaatctcat
    32101 ttctttaaac ataatatcat cctttctaat tttgatgctt aaattaaatt ttagaacatt
    32161 tataaagatt atgctatgtt ttttacaaat tttaagaaaa aaatatagtt taagataaaa
    32221 ggtagtgaaa gagaagctat ttggaggttt atttatgata ttattgttag agtagtataa
    32281 acaatactaa taaatttaat agctggtttc ttgttattta agatagtaag catgtctata
    32341 agaaggggaa gttatcgagg gtagaagttg ttatttataa ctatattata aagaaaatat
    32401 tattatataa aataataaat agtaaagagg tatcaagttg aatataatac ttaatacctc
    32461 ttcaaaatta agttataaaa gtgttgtggg aattgtccta tacatggtaa ggagataata
    32521 aagaatgtat ctttaaagtc tcaactaaac agcaaaattt atccaacaaa catacaataa
    32581 atgcttcttt gtatctaggt ggcttaacag taagtggcca catgtgtttt aaaattatgt
    32641 ccgtttcttt ttcatttatt tgaaaaaata aagttgcatt ttttaatgct tctctagggt
    32701 gagtaaatcc atgtaatcct tttctatcac ctttgtaatg ccaatcataa agaaaaaagt
    32761 cgtgtagtaa agctcctcta ataacacttt gaatatcaac acttaagtgc aattttttac
    32821 ataatagata actataatat gctactgaca aactatgttc aagacatgaa acattgccat
    32881 gatgaggaaa tgttctcata agttgaactt tttcagaaga aagtatctca gaagcacatt
    32941 cattaaaata tttgatttca gcttttgaca acattatgaa aatctccatt cttagtaata
    33001 tataaatact atacttcaac gataatatga aatatttctt atcctacaaa atataatacc
    33061 caataataca agtaagatac cttttatggt attttaaagt aagagagatt ttattaggct
    33121 aaacaatgaa aaaattaaaa gtaaagctat agcaatatat agtatttttc caagaggatt
    33181 gccaaaattt atagtccatc caataccaaa ccttttatta accattaatg atgggtcatt
    33241 tggattatta tacatgatgc ctgctatcca atacttctca tcttcttctg gagaataaga
    33301 tgagttgaat tttaaatttg gggaattaat atatgttgct attaaataaa ttataactaa
    33361 aagcgttgta atattcatag cagttgatag ttttgttttt attgataaaa gagtagtaaa
    33421 aaactgtgtt gtcattataa tcatcaatat taaaaacaaa taaccaattt tatttaaata
    33481 tttaatgttt tcaactctac tttcttcaat tctatttgta tctatcttaa tccttgactt
    33541 tatggatcca atggaagtta tatataataa aaccattaaa caaaagtcag taccgattaa
    33601 tttaaataca ttaagaaatg atttatctat aaaaacatca ggagacccat ttatgttcca
    33661 atgagtggca attgagtctg gcaattgatt ataattaaat gctataaaaa tactcattcc
    33721 caaagtaaat agtatcggta ttaaatagat tattttaaat ttttttatta ttttattttt
    33781 cttatttata aaatccatat ctactattaa ttttgaatct atatctatat gacctaattt
    33841 agaataaatt tctgatttaa gcttcttagc ttttttatgt gtatgaatat atagtatact
    33901 ttcatataat aaaaaaccta taataccaat aaaataggaa agttccaatt tattaaatgt
    33961 aaagaccaga ataaaaacta atataaagtc tataataaaa cctaaagata atagtttttt
    34021 aaattcttta tctaacttta taaagtaatt ataattttta tgctcttggt ctatagaaac
    34081 tccatagatg tgttttttac ctacaaaata gggcataaaa taaaatgtag catacatcaa
    34141 aattagaact ggaaatattg taaatagaca ttcaatctta ctcataatat ttcacctcat
    34201 acttgtcaaa taaacttgaa cttagtgaca aaaattcttc tttactaata cctgataaat
    34261 aagattttgc caccaataat tccaagtcaa gtttattttt ttgacgagtt gtattatcta
    34321 ttttcattgg tagttgagct acaatagcac cttttcttct atcaatattt aagaatccct
    34381 cttcttttaa ttcattgtag gatttgttca ctgtatgtaa atttacacca atattttcag
    34441 ccatacttct tacagatgga agtgattcat ttattttcaa ttctcctgaa gctatagcct
    34501 tagttatttc ttctttaatt tgtacataaa tagatttatc actattaaaa tctaaattta
    34561 aaaccataaa aaacctccat tatgctttat aaatgaaaac gattgttata tacatagtat
    34621 aacataaaaa ataataacaa acacaaaata taagtttata ttttgtaaaa attgtaattt
    34681 aaaaaataac ataattttta ttaaaataat acagatacta tttatagata gaaataaaaa
    34741 ttagaaagga gaaaaaatat gttaacatat ttaatgaaaa gacaaatgca taaaaaagat
    34801 gatggaatga gtaaaccaat gatgattaca ggagctgtaa ttacaggtgt aggagcatat
    34861 gctgtatata aaatggtaaa aagccataaa tctaattctc aaatggtcaa tacaaattat
    34921 tatggaaata gcgattatga tgattataaa tatgacgaat ttgattatga ctgtgattgt
    34981 agagacacaa aacaagatta tgaatatgaa gaattaaaaa aaaaagtaaa agaatttaat
    35041 tcaagaagaa taaattgtga aaattgtcca catgtttctc aagaagaaat gatgcaatat
    35101 gtaaatagtg ttaaagatgt aaaggaagag attgatttac atgaagatgc taaaaaagat
    35161 aatatttaca aagaagatga atacaaaaaa tatgaagaag aataagtaat attttgtata
    35221 actgaacaaa tagtaatgtc aaattattta atatctagtt ttaatatgta agttgtaact
    35281 taacttgaaa gaatcagtat cattctttag atagttaagt aacaacttac attttatttt
    35341 tataaatttt aatagttctt tgtgactatt ttgtaatttt agaaatattt tagtatccag
    35401 ggtaattgtg tataataaaa cttgtataga tagatagtat gaaattaagc agatagttta
    35461 aaattaaaat gaaaagaaag gaaatagtta tgttaaagtt gttgatagtt gaagatgata
    35521 gtactatatc ttttggaata aagtatgctt tagaacaaga aggatttagt atagatatat
    35581 caaaggattt atctagtgga aaagaaatga tttcttctaa tgaatatagc atgatactgt
    35641 tggatgttac attgccagat ggaactggtt atgagctttg tcaatatata agaggattct
    35701 ctcaggttcc tataatattc ttgactgctt gtgatgaaga agtaaatatt gtaatgggtc
    35761 tcgatatagg tggagatgat tatataacaa aaccttttag aattagagaa ttaatatcca
    35821 gaataaaagc tgttttaaga agaaaaggta atacttcaga agaaaataag aaaatactta
    35881 aatttggaga tttaagtata tatacattag aagctagagt atataaaaat gataaagaaa
    35941 tatttctgac atcagttgaa tataaacttc ttttaattct tatacagaat aaaaatactg
    36001 ttttaagtag aactcaaata ttggaaaaac tttgggatgt aacttatgat tttgtaaacg
    36061 acaatactct aactgtttat ataaaaaggc ttagagaaaa aataggtgat gatttatgtg
    36121 aaccaatcta catagaaact gtaaggggga ttggttataa atggatagga agtgaaaata
    36181 gtgtttctat ataatccaga agtgaagaag tttttaagta agtatgttac tttgatgttt
    36241 attgttatca taatatctat tggatttagc ataatcaacg tgtccttaac taaagagatg
    36301 gttgtaagaa ataatcaggc tataatagga actttatcaa gcaagtatcc aaacttggaa
    36361 agtcagatag tagacataat aactcaaggg aagtctatgg agaacataga ttatggtcag
    36421 aaaatattaa ttaagtataa ttatgataaa tctattagga taaactctga gcctattatt
    36481 tctaaattag ttttagagac tatcaaaata aatataatat tagtatgtat aatatttatt
    36541 ttgatatttg tgttggttgt tagatatttt aagtcaattt acaatgattt atcagatatg
    36601 actaagtatg tttactatag ttcagagggt aagccatttg atatgaaaaa caagaatcaa
    36661 gaagggcaga taggtcttct aaaaactgaa ctcttaaaga tgacaacaat tcttaatgaa
    36721 aaggttgaac ttttaaaaag agaaaagata tttttaaaca atacaatttc cgatatatca
    36781 catcaattaa aaactcctat gacttcttta ataatgttga atgatttatt atataatgat
    36841 gtaccatacg aagtaaaaat cgatttttta aataagataa aaaatcaatt aaatagaatg
    36901 gattggttga ttaaaagcat gcttaaatta tcaaaggtag aagcaaaagt gataaatttt
    36961 aaaaaagata aggtaaaatt tagtgaactc atacatagag caatgctatc tatgaaaata
    37021 cctatggaga ttaaaaatca aaaactgact atagagggaa atgataatgt atcttatatt
    37081 ggtgatattg attggtccgt ggaagcatta gtgaatataa tcaaaaattg tgtagaacat
    37141 actcctgaat ttggaaatat aattataacc tataaagaaa atccattatt ttctgaacta
    37201 ataataaaag atgagggtga aggtatagat aaaaaagata taccacatgt atttaaacga
    37261 ttctatagag gaagaagtag ttcaaaagaa gatagtgtag gaataggtct tgcaatgtca
    37321 aaatctataa tagaaagcca aaatggcgat atatatgtaa acagcgaaaa aggtcagggt
    37381 acagaatttc atataatatt tcataaaatg tatgatagtg actaatctgt aatatttaat
    37441 tcactttata gtaatagcca aaacctaata tatgaagtat agaatataaa tattggaggt
    37501 agttatggaa attttaagag tagaaaatct aactaaaagt tatggtaaaa atgaaacaaa
    37561 agttgatgct ataaaaaatg taagtttatc agttgaaaaa ggaacgttta tagcaataac
    37621 aggtccaagt ggaagtggta aaagtacctt attacattta ttaggaggag ttgacagacc
    37681 aactagtggt aaggtttata taaatgatgt tgatatatat aatttaaaaa ctaaggattt
    37741 ggcgatattt agaagaagaa atataggtct tatatatcaa ttttacaact taattccagt
    37801 cttaaatgtg aaggaaaaca tattgcttcc agcagagctt gacaatagag atattgatag
    37861 agaatatttt gatgatttaa tgaaaacatt aggtctaatt gatagagaaa agcaccttcc
    37921 aaaccaatta tcaggaggtc aacaacagag aacctctata ggacgtgcat taattaatag
    37981 accttctata atattagcag atgagccaac aggtaattta gatagtaaaa attcaaagga
    38041 aatattagaa ttgttaaaat tgtcagtaaa aaaatacaac cagacactaa taatgataac
    38101 tcatgacaaa aatatggcac ttcaagctga tagaattata agcatagaag atggaatcat
    38161 aaaaagtgat gaggtgatgt agatgaattt atatacatta ctaaccatta gatacttaaa
    38221 acaaaataaa agaagaacca ttgtaaccat aatcggtata atactttcaa cagctttaat
    38281 ttgtggtata ggaaatatat ttgaaagttt tatggattat caaatgagag aaactataaa
    38341 gaatgatgga agtttccatg ttacatttta tgatgtaaat agaaaaaatg tggaatatgt
    38401 aacgaaatca gctgaaatag aaaaacatgc atttaccaaa caacttggat attctaaatt
    38461 agaaaatagt gaaaatgcta tattaagtat aaagcaatat gataaaaatg caataaatgg
    38521 atataaagtt tctataaaag aaggtagatt tccagctaag gaaggagaaa ttgttctaag
    38581 tgaaagtatt attaatctta tagatgataa gttaaaaata ggagataaga taactttaaa
    38641 agtaggggat atgtttgact caagcaagaa aaagattgat agtatgacat ttcatgaagg
    38701 tgattatata gcaaatgaaa aagatagaac ttttaaagtt gttggtataa ttgaaaaacc
    38761 tgggtttgaa cgttataatg gaattgcaac agctatagcc tactttaatc ctatgaataa
    38821 ttacaatgat acaattaatg tatcagtagc agtaagaaat cctaaagatg tgtataagat
    38881 aagtaataaa ataagtaaaa atatatattc aaataaagat aaagaagcag tttctgatat
    38941 gataaaatac aatgaacatt tattgaggtt acaaggtgct agtaagtatt caaatataaa
    39001 taactctata aagtctataa ttatagcagt aactatttta gtaatcatat gtacaattgc
    39061 tacagtgtac aattccttta gtatttctat aaatgaacga aaaaaacagt ttgggatatt
    39121 aaattctata ggtgctacat ctagtcaaat taaaagatta gtttttatag aaggtattat
    39181 aattagttta attggtatac caataggact aatatctgga acagtagcta tagacctatt
    39241 gtttaaaatt ataaataagt attttactga atcggttgtt actaagatga gtttgcagat
    39301 agtgtataat ccaataataa ctattgttag tataataata gttttgctta caatctttat
    39361 atcaattcta ctacctgcaa tatcggcttc taatatttct caccttaatg tgattaaaaa
    39421 tactggagag tataaagtgg gcaaaatcaa aagctcaaga cttattaaaa tgatttttaa
    39481 aactgaagga gtacttgcat ataaaaatat taggagaaat agaaaaaaat ttataataac
    39541 tcttttttct ttaatggtaa gtataataat atttatatct tttagtggct ttacattact
    39601 tttactaaga ggtgaagaaa ttagaaattc tcaaagaaaa tatgatttgt atttaactgc
    39661 aaagaacaca gctaggtctg tagaggatac tataagtgaa ctagaagata ttgatgggat
    39721 aaaaaacttt gaattggcaa caggacaata tgtgtctata agagtaagtg aaaataaaat
    39781 aaataaatcc aaggaagatt taatcaggaa atattatcaa aaatataaga ttggagatag
    39841 ttatgaatat gatttcagta ataatgaatt aatttttcca ggagattttg cagtaaaaaa
    39901 caaaataaat aacttagtac agggctcttt taataaagag agagcaattg aagaaaatgg
    39961 tgttatatta gttagaaaaa gtgcatttga agaaaatggg aagaaaggtg tagtagaact
    40021 taccaactat aaagtaggag atacagtaaa ttgtgaatat cttgatgaaa atgccttaag
    40081 taaaaaagta aaggtcaaga ttcttgctat aactgacgaa gaacgtctag gtcttggtta
    40141 tcaaaatatg gggttacaat ttataactta tgatgaagtt gctaaaaatt taaatttaaa
    40201 attaagtaaa agccttatat ttattgattc aggaggaagc attaagacta agaaggaaat
    40261 agaagctttg gcaaataaaa ataattttaa cttttatgat gaaagtgcaa gcaatgaaag
    40321 tgaaaaacaa gatttaaagg ttattaaaat atttgtatat ggttttatag tagtgatatc
    40381 tttagtgagt gttacaaata ttctaaatac agtaagtaca agtataaatc taagaaaaag
    40441 agaacttgca gtaatacaat ctataggggt tactcctaaa ggatttagga aaatgatata
    40501 tttagaaagt tttatatatg gtatactgtc attgttgttt ggaataccaa ttagtattgg
    40561 tataattcta attatgaata aacttatttc aggtgtaatt gaattttctc cgattatacc
    40621 gtggacagcc atagttatct gtatagtaag tgtttttata ataactttta tagcagggta
    40681 tataccaatg agtaaattaa ataaagaaaa tataatagat aatataagaa gagaaagtat
    40741 atagatataa aaataattct taactatata aaatattatt atatcactta aatgattttt
    40801 tatttatata agaaatctaa taataaatta ctagaggtat ttaactattt agaaagaatt
    40861 ggtattatat aaagtaagag aatatataat aagaaagaat atttgatttt attggaaaac
    40921 aaaagaaaaa gaaggtgatt actatttctc atataactgc gaagaatatg tataaaagtt
    40981 tagaggaaag aataaacaag tttccacaag gtgctccacc atctgataca ttatacaaaa
    41041 tactaaatgt actatataca gaacaagaag cgaagttagt tgctcagctt ccaataaagc
    41101 ctttcagagt aaaaacagca gctaaaattt ggagtgtaag tgaaagtgaa gcatatagag
    41161 tattggataa acttgctagt aaggctctga tactagatat agaagataat aaagggaaga
    41221 aatatataat gcctcctcca atggctggtt tttttgagtt tgccatgatg agaacgagac
    41281 acgatataga ccaaaaactt ttggcagagc tttactatca atatatgaat gtagaagaag
    41341 attttatcaa agatttattt tattcaacag aaacgaagct tggaagagta tacgttcaag
    41401 aggaagtttt aactaatgat aatgaggtta gtattttaga ttatgaaagg gctactcata
    41461 taattgatga atcaactcat ataggtataa gtatgtgtta ctgtagacat agaatgcaac
    41521 atgttggaaa agcttgtgat gctcctatgg atatatgtat gacgtttgac aatgtagcaa
    41581 attctttaat aaataataaa tttgcaagac gtgtggataa aatagaatgt aaagaactac
    41641 ttcatcaagc atatgaacat aacttagttc aatgtggaga aaatgttagg aaaggggtta
    41701 cgtttatatg taattgctgt ggatgttgct gtgaagcaat ggtagcagct aaaagatttg
    41761 gaaatcttca tccagtacag acaacaagtt ttataccaaa tataaaccat gaaaattgtg
    41821 taaagtgtgg taaatgtata actgcatgtc caatagatgc gataagcaag gttaaggaag
    41881 atggtaaaga atatataaaa attgatgagg atagatgtct aggttgtgga gtttgtgtta
    41941 gaaattgcca taaaaatagc attatgttat taaagagaga tgaaaaaata ataactccag
    42001 caaattcagt acatagagca gttcttatgg caattgaaaa agggcagtta caaaatttaa
    42061 tttttgataa taatgctctg gctagtcata gagctatggg agctatactg tcagctatat
    42121 taaagcttga accagctaaa aagattttag ccagtaaaca attaaagtca gtatatttag
    42181 ataaattgtt aagtatgaat gataaataga aactaactta aaaaaggcaa agttttagac
    42241 ttatctatat cttaataaac ttatcattta atatttaaat ttaattttta cacatataaa
    42301 acttataact taaataaaaa aaggctatat tcaattaata ttacttgaat acagcctttt
    42361 ttagttatgt taagctattt cttttatttg cttaaatgag ttttcatttc catttccacc
    42421 aatttgtgtt acagatttaa atctcatagt tttaaatagt tctttttgat tataatctag
    42481 tgagtttcct actagcatta caggagattt agtttttcca gccaaaactc caacagataa
    42541 tccgtctatt aaatcatctt gtttattcat tccattttta gcaacatata aattgtctat
    42601 cttagaatcc ttgtaaaatt cttttattac ttttgcatta gtttcatttc tagtgctacc
    42661 tgatattctc tgtggatttt gaagtttact ttctatattt ttagaaactg tatattctcc
    42721 accgattaca taagatttat caatcttttt gtttttaaac aagttattta tttcagttat
    42781 attactattt tcattagtaa gtattatagg catatcattt tgagctgata cagctccaat
    42841 gcttactgca tcggctaatc ctttttcgcc attaactact gaaatctttg atatattgct
    42901 aatattgttc aattctttag caatgttcat agaagtttga tatctatcac ttcctgaaat
    42961 tctagataca gagatattgt ttgattttat agtatctaaa gatttttcat ttatagaagc
    43021 ttctccacca actacatata catttttaac ttttaatctc tttaattcag ccaaagtttt
    43081 ttcatttatt tgagagcttc cagtcaataa tataggagcg tttttcttgt atgcaaatgg
    43141 tgttgcagcc aaagcatctg caattgctga atcgtttatt aaaactgcat tttcagcatt
    43201 ttcccaacct gcttgactta tcttaataga agtttcatat ctatcagttc ctgtaagtgc
    43261 acttgcattt acttcatctt gatatgtaac ctcaaatcta gcatttggac ttcctaagaa
    43321 aacaattacg cctccttttg gcaatctaac tctattgttc tttgtaacta aagaatagtt
    43381 tacaggaaca cctttatcat cataaacagc aaaagctgaa ttttgaggaa gattaacttc
    43441 tattttttta tttgcgatat cgtcaccaat cttataccat tttgcataac cattggattc
    43501 tagttcacaa gtaaaagatt tttctgctgg caaattagtt atagaatctt cactaacata
    43561 cgttagtgtt ccaaaagata aatactctgt accattttct ttatgaagtt taatgtcgga
    43621 taaatcacga cctattacac caggtatttc tataaaagca tttgagttat tctcatccat
    43681 tattttagtg ttaactatat aacctggagt ttcatcagaa agactaaaac ttgcttttac
    43741 agagccaaac atatagcttt gagaagtata tttttcatcc actagatagt aacctttgcc
    43801 acttcttttt ttccacactt ctttgacact atttgatata ttgttgtcat ctactttttg
    43861 agctacatag tataaacttg ctgtctgacc taatccaggg acatcatcat aagagctcat
    43921 atttaaatat gtgatattat ttttttcttt tacaaatttt aaacaactat tacctttttc
    43981 agatacaaat ctatcttgac ctatgtatac gtatttatct tcaggtccat tttctatata
    44041 aggagaagat actgttaaag taccattatc attaacatct acttttatca tatttgaaga
    44101 agcataaaga ccactatttt cttttaaaga gctaggcatt ttaacttgtt gtggcttact
    44161 aaatgtttta tcaggtttta tttctttaat tttacctttt tcttttaatg cagatagtaa
    44221 gatttcttgt ccaattattt cattaagttg actacttcca ccagaagaaa gaactgcaac
    44281 tgccatattt tcatctggaa gaactataag attactgtgg aataataaag aatctccacc
    44341 ttttgtaaga gcttttaaat tatactgatt aaatggataa gtgttgacac aatcccaccc
    44401 taaaccatat ccaagtatag agtcttctcc ttcaggccat agaccattta aatattcttt
    44461 gttttccata gcttttactg aagcaggaga aagaatacca tttgaatttt tcataaatgt
    44521 ttgtgcaaat gtacatagat tttctgcact tgaatataaa ccacctgcac ctatagcatt
    44581 aaggttatct tgtggaactg catcttccca ataaggtaca tatgcttttg ctagttttga
    44641 actatcaaaa ctattttctg gagtttttgt attttgtaag ttaagtggat tatttatgta
    44701 tttatcaaga aaattagtaa atgacatacc agatactctt tctactaaaa tttcagctaa
    44761 agtaaatcca tcattacaat aaacagaaaa agctcctggt ttagctttta atctttgttt
    44821 ttgtaattct tttaagaaat tatcatgacc ataagaatca ttgtctgcta ataaaatagt
    44881 gtttttaaaa ctacttccca tcaaccctga tgagtgattt aataacattc ttggagtgat
    44941 ttccttgtat ctatcatcag ccattttaaa ttcaggtata tactttacaa caggagtatc
    45001 taaatttaat tttccatcat caaccaactt cataacagct gttgtagtaa acattttact
    45061 gatagatgcg atactataca tattatcctt atttaagttt ttgttatcct gtttgctgta
    45121 aactccacca ttaccagaaa tctcaatttt gccattatca atcaaagcat attgagcact
    45181 tacagcacca tactttgata gcaaagtttg gattttctct ttagcaagag ctttagtttt
    45241 tgtgtaagat tccgcgtttt tactataatc atgtaattct atatcagaac tttgatattt
    45301 ttcggaatta tcttttgtat tatcagcaaa agtaatattt ggagcagcag taaataacat
    45361 actcaatact aatgtactag ataataattt agagagcctt tgcttaaaca tattacatta
    45421 cctctttccc tttttaataa tttataatta gcataacatt cttaattatt ttaatcaaca
    45481 gattattatc aataaaaaaa gcttttataa gtatagcttt ttatataaca gagaaaaata
    45541 taaaaataat atttgttgtt gtataaagaa taaagggtat aggtaatata taaataaaat
    45601 ggaggaattt tagacatatg tataagaata taaataagaa gcaacttaaa gaaatgatga
    45661 aaaatgaaaa ggatgtttta ttattagatg taagaactaa agatgaattt aaggaatata
    45721 aaatagaaaa tgctataaat atatctttac aagagttaat caataatata gatgaaattt
    45781 acgattataa agataagaaa gtagtagttt attgcagaag tggtcataga agtgttactg
    45841 catgtaattt gttagctgaa gaaggttttg agcatttata taatttaaat tcaggaataa
    45901 ttgattatat gagttaaata atgtaagtta tacattaaat actatgtata gctctgctta
    45961 ttaagtagga atatatctaa atatttataa gataaattta ggaaagaacg aattttttcg
    46021 tttttttcta tgacagtttt tgtagtaaat attagttttt aagttggaaa aaggtagtag
    46081 ctttttagct actacctttt aatatgaact tagaatatta ttaaaaaaat atatttaaag
    46141 acagaattaa ttttttaatg accagttaaa ttactatcgc cgaatttatc attaaatggt
    46201 tttataactc tatttatagg tccttttaga agtacattta aacaaataga cagaatcata
    46261 aatagtaata gaactagtat atctttagtt agatttggag catatatacc accaactgct
    46321 tcccttgtag cacctattgc ataggtaaat ggcattatag ggtttattct ttggaagaat
    46381 gtaggtgtaa cttgtatagg gaatgtacca ccagaagaac caatctgaac gactaatagt
    46441 actattgcaa ttgcttttcc tacatttcca aatacagaaa ctaaagaata cactataaag
    46501 ttaaatacca tactagtaaa cataagcaga actatcaata aaattggatg ttttgctgtt
    46561 actcctaaca ataatatatc tccagttcct attataaaac cttggacaag agctatactt
    46621 aaaaaagtaa ggcctcttcc aaagtaaatt tcataaggtt tatagtttcc atgtacagat
    46681 gtagacaata gtgctgaaat aagtaatata ccaacccatg tagaaagaac actataaaat
    46741 ggtgtcattg cagaaccata attttcaatt ggatataatt tctctgtttt cacctcaaca
    46801 ggttctttta agaaattaga ctttgtcaat atatcacttt caagcaagct gactaatttt
    46861 ttcatatctt caccattact aattttgctt aaagtatcaa tcaaatcatc caacattcct
    46921 tttgctaaag gaagtctttc tcttatcaat gatatacttt cctgtgcatt tcctgaaagt
    46981 tttagagaag tagtaagtat ttcttcaacc tttggaagtt ttgcttctgc cttatctaaa
    47041 acagtaatta tatcatttgc tacttttata ctgtttgcaa atatactatt tataggtttt
    47101 gatatttttg agtcaaaatt atttaaaata tttaaattaa ttcttccaat accatttgat
    47161 agacttaaaa cattattcaa agcacttata gatggttgtt gtccagatat aactttgttt
    47221 ttaatatcat ttaatgttga tatagatgaa tctattttat tgctagagtc ttcaagatta
    47281 tctatgacat catccaatct attatttgat gtaagttggt ttaatttagt taaaaaatcc
    47341 accaaagtat catttaaaga ttgcaaatta gaaagttttt ctgataaatt atctatcaac
    47401 ttaggtacat cttcactgcc actatttact gcatcaatta aatttaatgt aagagaagaa
    47461 gcagaagatg aaaggtcaac cattaaattt aagtcagact taataagagg agaaagttcg
    47521 tctaaattat catttgtatc ctctaagaat ttttttaagt cacttgataa tagttttgta
    47581 tcattcagag tctttttaat tgtaggtaaa tcagatttaa tatctttaac tgtatcagat
    47641 aattttgaag tagcatcaga agctaaatca acagtcttat ttatatggtc aaatctcttt
    47701 tgaaggtcaa taagggaatt ttcaactttt gtcaattttg gaagactgtg ttcaattcct
    47761 gcaccaactc cgtttgatat accaagaacg atttcactaa ctgtttttac tatagtctga
    47821 ttaacctgta gttgtattgt agaagcacct ttatctgtaa tctttggtgc aactgcattt
    47881 attttttcat taacagtata gataatttcg ccttttttaa catcatctgt aattaaagat
    47941 gtcaaatctt ttgtaaaatt ttttggaatc ttaagtgatg cataataagt acctttttta
    48001 acaccttcaa gagcagtttt ttcatctaca aactgccaac ctaaactctt gttatctttg
    48061 agttgttcaa ttaattggtc tcctacatta atcttttgtc ctgctatttc atttccttta
    48121 tcttcatttg ttactgctac aagcatatta gcagtgcttc cataagggtc ccaagatgcc
    48181 tttatattga accaagcata cagagatggt aaaaaagcaa gacctaaaac tattacaagt
    48241 acggcataat tgccacgtat atcttttagg tcacttttaa aaatttctaa tatatttttt
    48301 aaaccctttg aaggtcttaa attcttgata ttattccagt ttaaaatttc ttttaaacta
    48361 ttagtttttt tcaaatgtct cacctcctaa aatattttga taaagaaaaa agttagattt
    48421 ataatcaatt ttaaattata ttacttttta cac
//
LOCUS       DACULQ010000006        47885 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.006, whole genome shotgun sequence.
ACCESSION   DACULQ010000006 DACULQ010000000
VERSION     DACULQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.006"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     regulatory      318..403
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            565..1086
                     /locus_tag="JA154_01385"
     CDS             565..1086
                     /locus_tag="JA154_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HAT7994510.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTTTDKAGLTILETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTDTEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGDELTDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            1238..2914
                     /locus_tag="JA154_01390"
     CDS             1238..2914
                     /locus_tag="JA154_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAT7994511.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            2926..4134
                     /locus_tag="JA154_01395"
     CDS             2926..4134
                     /locus_tag="JA154_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HAT7994512.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            4149..5861
                     /locus_tag="JA154_01400"
     CDS             4149..5861
                     /locus_tag="JA154_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994513.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            5854..6762
                     /locus_tag="JA154_01405"
     CDS             5854..6762
                     /locus_tag="JA154_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAT7994514.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            6786..7355
                     /locus_tag="JA154_01410"
     CDS             6786..7355
                     /locus_tag="JA154_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin"
                     /protein_id="HAT7994515.1"
                     /translation="MMSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKK
                     QQIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKK
                     NTEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSI
                     YYNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            7392..7919
                     /locus_tag="JA154_01415"
     CDS             7392..7919
                     /locus_tag="JA154_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin"
                     /protein_id="HAT7994516.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            7940..9478
                     /locus_tag="JA154_01420"
     CDS             7940..9478
                     /locus_tag="JA154_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994517.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRNSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKKFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            9578..10660
                     /locus_tag="JA154_01425"
     CDS             9578..10660
                     /locus_tag="JA154_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HAT7994518.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            10641..11423
                     /locus_tag="JA154_01430"
     CDS             10641..11423
                     /locus_tag="JA154_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAT7994519.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILSGKQLLELYFSMVI"
     gene            11513..12175
                     /locus_tag="JA154_01435"
     CDS             11513..12175
                     /locus_tag="JA154_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAT7994520.1"
                     /translation="MLQTMEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIIT
                     VVLSVLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLA
                     FFNIVQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIV
                     LSFILAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYK
                     FI"
     gene            12189..12749
                     /locus_tag="JA154_01440"
     CDS             12189..12749
                     /locus_tag="JA154_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAT7994521.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            12766..16152
                     /locus_tag="JA154_01445"
     CDS             12766..16152
                     /locus_tag="JA154_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAT7994522.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMEQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            16183..17178
                     /locus_tag="JA154_01450"
     CDS             16183..17178
                     /locus_tag="JA154_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAT7994523.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            17302..17841
                     /locus_tag="JA154_01455"
     CDS             17302..17841
                     /locus_tag="JA154_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HAT7994524.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            17961..19574
                     /locus_tag="JA154_01460"
     CDS             17961..19574
                     /locus_tag="JA154_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAT7994525.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            19681..21129
                     /locus_tag="JA154_01465"
     CDS             19681..21129
                     /locus_tag="JA154_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAT7994526.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            21218..21496
                     /locus_tag="JA154_01470"
     CDS             21218..21496
                     /locus_tag="JA154_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT7994527.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            21568..21810
                     /locus_tag="JA154_01475"
     CDS             21568..21810
                     /locus_tag="JA154_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAT7994528.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYKILE"
     gene            21872..22126
                     /locus_tag="JA154_01480"
     CDS             21872..22126
                     /locus_tag="JA154_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HAT7994529.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            22127..22660
                     /locus_tag="JA154_01485"
     CDS             22127..22660
                     /locus_tag="JA154_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994530.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            22666..22950
                     /locus_tag="JA154_01490"
     CDS             22666..22950
                     /locus_tag="JA154_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAT7994531.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            23063..23983
                     /locus_tag="JA154_01495"
     CDS             23063..23983
                     /locus_tag="JA154_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HAT7994532.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            24073..26445
                     /locus_tag="JA154_01500"
     CDS             24073..26445
                     /locus_tag="JA154_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HAT7994533.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            26916..28688
                     /locus_tag="JA154_01505"
     CDS             26916..28688
                     /locus_tag="JA154_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAT7994534.1"
                     /translation="MNNNDTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDK
                     DTKELKNYIGKVTKSPTHLSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMND
                     SMSKSYGNYMGICSDLELEILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDD
                     KSEDILSKVSSISSLPSQVYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELI
                     TYNDNINTIAGLITGQVKKNIFYSQERGYESSLEMYLEADNVDEKVYNNLIRTVNSNM
                     DSLHKYIDLRKKVLKLDKVHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYND
                     VVYKAFNEKWIDVYSHENKVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVY
                     EYLSSKNQNYFNSNPSIFTHEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKD
                     TLYTQTMYAEFEKTIHELVEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWAR
                     IPHFYNSFYVYKYATGCSAGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINL
                     TNTKPIENTIKKFDSLVAELEKLL"
     gene            28802..29767
                     /locus_tag="JA154_01510"
     CDS             28802..29767
                     /locus_tag="JA154_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HAT7994535.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            30282..31871
                     /locus_tag="JA154_01515"
     CDS             30282..31871
                     /locus_tag="JA154_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAT7994536.1"
                     /translation="MILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNELIEIIN
                     SKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKEIDTQN
                     ISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIVDEFNT
                     SKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGITRHPK
                     SGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRIIDLIS
                     PIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDMKESIE
                     GDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLTISPTG
                     RTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEFKGTGN
                     MELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVMEITDK
                     VIELIKRTKDNKEFVKSIKNL"
     gene            32024..32224
                     /locus_tag="JA154_01520"
     CDS             32024..32224
                     /locus_tag="JA154_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAT7994537.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            32369..33262
                     /locus_tag="JA154_01525"
     CDS             32369..33262
                     /locus_tag="JA154_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HAT7994538.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            33280..34128
                     /locus_tag="JA154_01530"
     CDS             33280..34128
                     /locus_tag="JA154_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-(glutamine-N5) methyltransferase,
                     release factor-specific"
                     /protein_id="HAT7994539.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            34172..35236
                     /locus_tag="JA154_01535"
     CDS             34172..35236
                     /locus_tag="JA154_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAT7994540.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            35365..36045
                     /locus_tag="JA154_01540"
     CDS             35365..36045
                     /locus_tag="JA154_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAT7994541.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            36074..37114
                     /locus_tag="JA154_01545"
     CDS             36074..37114
                     /locus_tag="JA154_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAT7994542.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            37131..37580
                     /locus_tag="JA154_01550"
     CDS             37131..37580
                     /locus_tag="JA154_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HAT7994543.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            37597..38049
                     /locus_tag="JA154_01555"
     CDS             37597..38049
                     /locus_tag="JA154_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HAT7994544.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            38085..38714
                     /gene="upp"
                     /locus_tag="JA154_01560"
     CDS             38085..38714
                     /gene="upp"
                     /locus_tag="JA154_01560"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089999.1"
                     /note="Catalyzes the formation of uracil and
                     5-phospho-alpha-D-ribosy 1-diphosphate from UMP and
                     diphosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAT7994545.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            39015..39830
                     /locus_tag="JA154_01565"
     CDS             39015..39830
                     /locus_tag="JA154_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum hydroxylase"
                     /protein_id="HAT7994546.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            complement(39827..40084)
                     /locus_tag="JA154_01570"
     CDS             complement(39827..40084)
                     /locus_tag="JA154_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994547.1"
                     /translation="MKYSSHDGLIIYFSPLCLCTGIIYLLDFNLNKYLFLYYIHMLIE
                     CPIKKEHFYHLNFKYPSYALFNLLNLLYHLNKILIALSNIN"
     gene            40056..40493
                     /locus_tag="JA154_01575"
     CDS             40056..40493
                     /locus_tag="JA154_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HAT7994548.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            40678..40905
                     /locus_tag="JA154_01580"
     CDS             40678..40905
                     /locus_tag="JA154_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994549.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            40910..41296
                     /locus_tag="JA154_01585"
     CDS             40910..41296
                     /locus_tag="JA154_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HAT7994550.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            41299..42003
                     /locus_tag="JA154_01590"
     CDS             41299..42003
                     /locus_tag="JA154_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAT7994551.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            42072..42332
                     /locus_tag="JA154_01595"
     CDS             42072..42332
                     /locus_tag="JA154_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAT7994552.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            42455..42952
                     /locus_tag="JA154_01600"
     CDS             42455..42952
                     /locus_tag="JA154_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAT7994553.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLVEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            42949..43494
                     /locus_tag="JA154_01605"
     CDS             42949..43494
                     /locus_tag="JA154_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAT7994554.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            43511..45013
                     /locus_tag="JA154_01610"
     CDS             43511..45013
                     /locus_tag="JA154_01610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089990.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAT7994555.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            45041..45919
                     /locus_tag="JA154_01615"
     CDS             45041..45919
                     /locus_tag="JA154_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAT7994556.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            45932..47326
                     /locus_tag="JA154_01620"
     CDS             45932..47326
                     /locus_tag="JA154_01620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089988.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAT7994557.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            47329..47589
                     /locus_tag="JA154_01625"
     CDS             47329..47589
                     /locus_tag="JA154_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994558.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        19375 a         5101 c         8917 g        14492 t
ORIGIN      
        1 atagatagaa tgtttataat agatgatttt ataaaatact atattgtaat agcttttagc
       61 tttcaaataa atattagcaa aggatggaaa tgacaatgat aaaactaaat gataatttat
      121 aaatatctcc ttacgatata taataattgt agggagatgt ttcttttttt tgtagagtat
      181 aatttttcta gaaattctgt ttcaatatgt aaaaagttgg tatatataaa tagtataaac
      241 agtgttgcta aaatatggta gatattatat aatataaatg gatatgtata tacattatga
      301 gttatataat ttgaatttaa taaaatagaa acgttgattt atgttctgta atgtgggcac
      361 cttggagcat attgagttag tggtgcaacc ggctatgaaa ttgtatttat ttataaatac
      421 tattattttc atagcttttt ttatttaaaa aatggttata tgttagaaat aaatttggtt
      481 agtacgttta aataatttta cttagatttt taaccagata tattataagt aagtattaat
      541 taaaaaattt aagggggaat aaaaatgaag ttaaaaaaga ataaaaaagg tttcacttta
      601 gtggaattat tggtagtaat tgcaattata ggtatattag cagtagtggc agttccagct
      661 ttatttagta atataaacaa ggctaaggta gcaagtgttg agtctgatta tagctcagtt
      721 aagagtgcag cattatctta ttattcagat actaataaga taccagttac aacaactgat
      781 aaagctggtt taactatttt agagacttat atggagtctt tgcctgataa agctgatata
      841 ggtggaaaat ataaattgat taaagttggt aataaattgg tattacagat aggtacagat
      901 actgaaggag ttacattaac agaagcacaa tcagcaaaat tattgagtga tataggtaaa
      961 gataaaatat atacaggtgt tacaggagat aattttggag acgaattaac agatactaca
     1021 aaaatagata ataaagctct atatatagta cttatagata atactgtgat ggactcaaca
     1081 aaataattaa aactatattt ttataaagaa tattgtaaat ttaatgaaaa atataatata
     1141 aaagacagga agaaaatctt cctgtttttt tatactattt tctttaatgt tcaattatga
     1201 atgatgatat aattgagata gtaaaggagt gaaacctgtg gctaaaaaag ttagaatagg
     1261 agacaaactg gtcgaaaaag gatatataac agaagaacag cttaaatggg cactatcaga
     1321 acaaaaaaac agtggaaaaa gactaggaga atttctagta caagaagggc ttatagatag
     1381 taacttacta ataagtgtat taaaagaact cttggatata gaaagtatat ttttagaagg
     1441 gacagaaata gacactttag ctacaaaaat ggttccagaa aatatatgca aaagatatac
     1501 tgtatttcca tttaaaattg atggaaataa aatatgtcta gccatgtcag acccacaaga
     1561 tagagaagca gttcaagatg taaggcgtat gtctggtaaa gacgtagaga tatttatatc
     1621 aagtacagaa gatataaaca aagcaatagg tcatgcttat gcacattcag aaataaataa
     1681 ggcaatgact gagtacaaca aaaatagaac tggtggagta agagaaacag taatacttga
     1741 agaagatgta aatgctgctc caatagtacg attagtaaat aacatactag aaaatgctgt
     1801 tagaatggaa gcaagtgata tacatataga acaaagtgaa aattacatgc gtgttaggtt
     1861 taggatagat ggaatgttaa gagaatatat gaggatgaac tcagcaccat ataaggcagt
     1921 aatcagccgt atcaagataa tgtcagatat aaatatatct gaaaagagaa taccacagga
     1981 tggtagaatc tatttaaaag tggataataa acccatagat tttagggttt ctacaatgcc
     2041 aactaacagg gatgaaaaga tagctatgag ggttcttgat aagagtaatt ttatggttag
     2101 taaagaagta ctaggaatag acgaacatgg ctctaagata tatgatgaat tgataaacac
     2161 tccttatggt cttatattgg ttgttggccc tactggaagt gggaaaacca ctacattgta
     2221 ttcaatgtta aatcaactaa atacagaaaa tagaaatcta ttaacaatag aagaccctat
     2281 agagtatgaa ttacctggtg taaatcaatc tcaaataaat gaaaaggcag ggcttacttt
     2341 cgccagcgga cttagagctt ttatgcgtca agacccagat ataataatgg ttggagagat
     2401 aagagatact gagacagcag aaatagcaat tagagcttct cttacaggac atttagtttt
     2461 aagtacatta catgcaaata cagcagttgg agcaatttca cgtcttttag atatggatgt
     2521 ggaaagtttt ttaataactt catctgtttt aggtgtaata tctcaaagac ttacacgaaa
     2581 aatatgtgag cattgtaagg tttcatatga agcagatatt ggagaaaaaa aggctcttgg
     2641 tattgatgta aatgaatcag taactattta tagaggaaaa ggttgtgaaa ggtgtaataa
     2701 tactggatat aaaggacgtc taggtatatt tgaaatgctt gaaattactc cagaaataaa
     2761 agagttaata gattcatctg caaatcaaag agaaatttta aagatggcta gaaaacaagg
     2821 tatggttagc ttaaaagagg atatagttaa aaaagtttta aatggaaaga caacagtaga
     2881 agaaatgatt agaataattt tgatgactga ttaaaggatg atattatgaa aagttttaaa
     2941 tacacagtaa taaaagagga cggaaaaaag caaactgatg tgattgaagc caacgatttt
     3001 aatgaagcta gaaatcttct tagaaagaga aatcttagag ttattgaaat caaagaaagc
     3061 aaaaataaaa atataagatt atctagtaga aagagaaaaa aagacttagg agctgaccag
     3121 ataagccact tttgtagaca atttgctatt atagtatctt ctggtataaa tagtatttct
     3181 ggattagaaa cattagctag aaggtcaact aacattacat taagagaaga aattaatcgt
     3241 atagtagcag aaataaaagt aggttctact attgcagatt caatgctttc atcaaaatca
     3301 aagtttccaa agttacttgg tgctatggta gctacaggtg aagctacagg taaattagat
     3361 gaagtattaa aaagtatggc ttcattttat gcaagtgaac acagggttaa acaaaagctt
     3421 agaaatgctg caacatatcc attaatagtt ttgatttctt catttataat gatttttata
     3481 tttacaacat ttatggtgcc taagatgatt aattcaatta caacagtagg agcaactctt
     3541 ccacttataa caagaattgt tatggggttt ggtatgttta tgaagaagtt ttggataata
     3601 gtacttttag ttattatatt atttatctat cagtttaaaa aatatctaaa aacgtcagtt
     3661 ggaagggctc ataaagatag agttataaat aagatacctg ttttaggaaa aggaataaat
     3721 tgtatggtag ctacacgatt ttcaagagca ttgtatctat ttgtatcaac aggataccca
     3781 ttagttcaag ggcttgatta tattatagat agtgtaaata acactatggc agagaagatt
     3841 ttagcatcag ctaaagatgg aataacaaga ggagaaggct tagcagaaaa tttagagaga
     3901 tatacgtatt ttgactctgt gttggtacag atgatagcta taggggagca gacaggggaa
     3961 ttagagaata taagtagaca gatggcagaa ttttatgagt atgaatccga gatttattta
     4021 aataggatgg cctcaatgat agagcctgtt ttaataattg cagttggtat tatagttgcg
     4081 atactggttg tatcaatatt catgccaatg cttagtatat atgatgccat gtaggaggga
     4141 aaggacaaat gaacaaggtt tatgtatcat attatggcga ttctatttcc atagttgagg
     4201 gcagatataa acacgataag gataagtttt atgtaaaaaa ttttaatgtg ttttcaatag
     4261 aggatattgc acaagatttt tctaaggaaa agtatgctct attgagatac gcattagaaa
     4321 atgttaagtt aaaatcaaga catgtagttt tttgtttaaa tacaagagat gtaattttaa
     4381 aaccccaaaa acttcctaat atggacaaaa aggatttaga cggatttatg aagctagaga
     4441 tggacgaaat gatggctttg gagtcagatg aatatgtatt ttcgtatgaa gtatctagtg
     4501 aaacacctga taattcagat ggcgatgaat caagcttaaa tcttattatt ggagctatac
     4561 aaaaggaaga ggtaaatgca attgtagaga taatgcatga atttgattta gtattagata
     4621 gaatagatac attatcaaca gcttacttaa gaatgcttaa aaatttagac tacaatgata
     4681 taatggtaac caatataagt gacaatagca caattataaa tatatataag aaagatactt
     4741 tatttattag tgataatata cctataaaga taactgctaa agatatggac atgcaattat
     4801 taaatgtagt agaagaatca aaaggtctta tgaattacta ttcgtcaaga aactttggta
     4861 aggttacgga tactatatta atattaggaa aaagatattc caataaatta atttatgaaa
     4921 cttttagctc tgcattttca agctatgttt ctcaaggcct tactgagata atggatatat
     4981 ctgatatgat aactggagat atagatgaag atgatataaa ttcaattgta gagcttttag
     5041 gaagtatgat agaacctaaa gggaaaagag attttgaaaa tataaacttt ttaccaatgg
     5101 atatacttag aaaacagata aaagctaaac aaatgaaaaa catactgaaa attgctccaa
     5161 tagttatttt gattcttgct ataccttatt tatgtttaat tttttcaaat aatttagcaa
     5221 ataaggaact tgaaaatgtt aatggtgaaa taagaaatat tgaatctgca tattatcaga
     5281 ttggaaatat agaaaaaatg atacagagta aaagcgaaga aataaaggta tatgatatgt
     5341 taattgggaa aaaggtaaag tgggaaaatt tattggatga tatagataaa aatactccaa
     5401 gtaaagttga actaacaagt ttatcaacaa cttatcaaaa tcttggaact aataataaag
     5461 ctgataattc aaaaagtaat aagactacaa cagataaatc aagtaacaat gaaacaaaat
     5521 caagtacgaa aagtgatagt aatggagata aaacagataa tgatttaaat gaagataaga
     5581 ctacaaatgt gtcagaagca ttaactggaa atagtgagac taatagtaat aacacggata
     5641 aagatgataa aactccattg tatgataaga taccaaatgt tataaatata gaaggaaatg
     5701 ctaaagattc ctcttatgta ggtcaatttt tatataaatt aaagggatta tactatttca
     5761 aggatgttaa attacatagc ataaaaagta atgcagaaga ttctaactat acatttagta
     5821 tgacattata cctgaaagaa ggtgtattaa ctaatgaata aagatatatt taaaaaagat
     5881 ataaaagata tatttaaaat ggaagttagt ctaaaaggga ttcttataaa ggttataatt
     5941 ttattagcta ttttatcata tggttatttt ggactttacc ctcaatatca gaaatatatt
     6001 gctacaaaaa attctgtaaa acaggctact gaaaaattaa atatatataa agagaaaact
     6061 gcacttatgc caaaatatga aaaacaactt gaggaagttg aaagtgaatt aaaagaaaaa
     6121 agcaaaaatc ttttatatga tatgcaagat ggtctttttt taataggttt agataaaaaa
     6181 atgaaggctt ataatattga cttaaccagt tatgacgttg gaaatgttaa agaatacgaa
     6241 catttttatg gtatatcaac aacaataagt gttagaggtg attatagaaa tgtcaaaaaa
     6301 ttaatggctt atttagaaga acaaaaaaat gttactcaaa tactagatta tacaatgact
     6361 tcatatatag aaccagaaaa agaagaggaa gaaacaacaa acaataagga tttaattaaa
     6421 aatgtgaaca taaataaaga tacaaaaata tactgggtgg aaggcgaaaa taagtggcat
     6481 ttatataagg attgtccatt gataaaagag ggagagatta taaaaaatga caaactttta
     6541 gataggtcta ctaattatct atgtaatact tgtgtactta gagctgcaac agaaaaagaa
     6601 gcaaaaaaag aagtcgtagc tccaaaggca gaaggtgtag tagatgctac atttaaattt
     6661 ataatgtaca ctaaagagaa tactacaatg atacttgata cagataatcc atcttcatgg
     6721 agaccaggaa aatataatcc atttaagtct agtgcgaaat aaagctaagt aagctttaaa
     6781 gataggtgat gagtttgtac aaaaataatg aaaaaggtct tactttatta gaagtaataa
     6841 tagctgtttt tatactgaca atagttttaa gtatttctta taaagtgttc aatggtataa
     6901 catcagcagt aaaaaagcaa cagattatta cagatgctca agtaaatatt aatctaatta
     6961 ataagtatct aaatagagat ttggaaaact gtaaagagct aactaaaact ggttcaggta
     7021 ataattatga atacaatata gagatgccag ataatgttgt aaaatatgaa gtttctatag
     7081 aaactaaaaa aaatacagag gtatattctg taacgagaat ccaaaacaat acaattgata
     7141 cagaaaatga agttagagaa gagataatct ataatcaacc actagttcaa aataataaag
     7201 aaatgaagga aacaccattt aaaatagaaa agcaaactgg taaatctatt tatactgtaa
     7261 gtatatacta taatgaatcg gtgcaagaaa gtcataaaaa tagcaattta aataacaaaa
     7321 cttatacatt tgatgttatg tcaagaatag ggtaataaga atttattaaa aaataagtag
     7381 gttgataaaa tatgagtaaa aagaatagtg aaagaggatt ttccttaatt gaagtgttag
     7441 tagctatggc tattatggga attgtactat ttgcattttt taatattatc aatactaata
     7501 acaaagcaaa cactaaaaat gacacagata taacttcctt aaactatgtc caaagtgaaa
     7561 tagaaaatct aagagaaaag ataaaaagtg gagaatttga ttttgatagt ttagataaac
     7621 tagaagatgg aactgtcgta tatgaaaaat taatagataa atcaaaaaaa gttgtatatg
     7681 ataaagttct tagtgaaggt gatgtgagct tatatgatac tccatatgaa aagattacaa
     7741 caataaaaga tgaagatggt aatttaatcg acaaaggaaa tattacaaat aagataaaga
     7801 ctattgtaga agataaaagt ggtcagatat ataaaatagc tgtaactggg aaaagcatga
     7861 atgactattc aagtaaaaaa gaggttaaaa ttgtaactga aatatttaaa gataaataat
     7921 tttacttggg ggtgagattt tgaggaagtg gaataaattt aaaagtgaaa gaggggcagc
     7981 tttagtttta gttttggttg tagtagcatt gctttctatt gttggattga tattttcaaa
     8041 tcagatagct aatagaataa aatctacaaa gactactaat gaaggtatac aagcaaagta
     8101 tttagcagag acatgtgtgg aaaatagtat tgataaagca tatgaaaaat tgtatgatga
     8161 gcttgagaaa atggataatg agtttaaaag tgaaaatcaa gaaaaatcca tatctaggtc
     8221 aaaattaaga aatatatcag atgaagattt taataatcaa gatgaggaaa atattgaagc
     8281 agaaagactt ggatatatga ataatataaa tttctattta aataaagctt cttcagactt
     8341 ggaaaaagct agtatggaat tgaaaaagct atatgattta gatatgttgg actatagaga
     8401 cattgaatat gtagatgcaa atattatttc acatagaaat tctatcttag aaatttgcaa
     8461 gaattatact tctggagata ttagtaaaat aaatgaatat attctgaaag aagatataga
     8521 ctcaactaca ttgattgagg caaagttagt gaataatgat attcttttaa aaaagttttt
     8581 agaagaaaat aaaattgaaa atgaacattt aaattcagcc ttttcacata cttataaggc
     8641 attagataac atttctttgg cgatgcaaaa tatgattgag tatagacata cgtttcatat
     8701 agatgagcca aaagtagaag tatctaatgg gattccagac tctcaacaat attatgaact
     8761 aatacaaaat cctataataa atagtatgga gtatagctgg aatagtaaat gggatacatt
     8821 agaaaaccta ttggaaattt taccaaatca aactcaagga tttaattctt taagagttca
     8881 tttgagaaat aatgtaagaa aatttgagaa gttaagcgat aatatatcta gtggtaaaaa
     8941 aaatacagca aaaaatttct taaagtataa agagttattg tatgaaatat cagaccaatg
     9001 taatcaacta aaaagtatgt cttatgagaa aatacctgtt aaatatgata acatggcttt
     9061 gataacaact tttgactata tacaaaatga gcttttagca gagataaaat gcagattaaa
     9121 agaattaaaa ccacaagaaa ttgataaaac agaaggtatt actataaaaa taccttttta
     9181 taaggctgac tacgacatga caaaagaagg atggcctaaa ttaaaagaaa atggttcagg
     9241 ggcggagtta agcttaatgg taactggtga taaaaatgga ataaaagaag tagaagttac
     9301 agatggtaaa aaaaatatta ttggtttagg tgttgaagaa aattcaaatt caaagtataa
     9361 ggttgacgcc attgtaaact ttaatctaaa tatagataca aatgtagttg gaaattatga
     9421 tataaaagat aaaattttga ttaatcatga tataagttca tacaaaaaag taaactaaat
     9481 ataagaagca acttagtata aataaataat aaaataattt tattatttac tactaaaaaa
     9541 ataagtaaac taatagaaga tatggagagt aaatattatg tatatttatg atttattaga
     9601 gcaaggtata agactaaacg catcagatat acacataaca gttggaacaa atcctgtagc
     9661 tagagtaaaa ggtggatttg taaaactaag tgaacaaata ttgacatcag aagtaacaat
     9721 gcaaatggca aaagacatag caggggaatc tatgtttaaa gtaatagaag aacatggaga
     9781 agctgatttt tcagcttctc taaaaacagg agaaagattt agggtaaatg catataggca
     9841 aaaaggaaat tacgcaatag caataaggac tataacagca gaaattccaa catttgaaaa
     9901 attaggcctt ccagagagta taaaatcttt tactgaaaag cataaaggat tagttttagt
     9961 tacaggtcca acaggaagtg gtaaaagtac aacacttgca agtatgataa atataataaa
    10021 tgaaaaacaa caaaaacata taattacact tgaagacccg atagaatatg tacatcatca
    10081 taaacaaagt ttagtaaatc aaagagaagt aggaactgat acagaaagtt ttcattcagc
    10141 ccttagagct attttgcgtc aggacccaga tgtaatacta ataggagaga tgagagaccc
    10201 tgaaactgtt tcaatagcat taacagcagc agaaacaggt catttagtat tttcaacact
    10261 acatacagta ggggcagcta agacaattga tagaatagta gacatgtttc aaccatccca
    10321 acagcaacaa ataaaaacac agttatctac agtttgtgaa ggtgtggtat cacagcagtt
    10381 acttcctaca gcagatggga aaggcagaat agcagcaatt gaattgatgt ttgctactcc
    10441 agcaataaaa aacctgataa gagaaggcaa aacatatcaa attccaaaca tgatacaaac
    10501 tggagttaaa gtaggtatga aaactatgga ccaagattta atggaacttt ataaaaatgg
    10561 aaagattaca aaagatatgg cacttagtag gtgtactgac caagagttta tgacaagaat
    10621 gataggtgga gtaaactaca atggatatta taatcgttaa tctatatgta tttatagtgg
    10681 gtatagtgtt tggaagtttc tttaacgtct gtatttttag gattccagaa ggtatatcta
    10741 tttctagacc accttctcat tgtatgagtt gtaatactag actaaagcca atagacttaa
    10801 taccagtgct aagctattta atgagtggca gaaagtgtag atattgttat gagaaaattt
    10861 ctagtagata tgctatagtg gaattaatta caggactttt atttttaaca gtatttatgt
    10921 tatatggaat atctacatca actatttatt atttggttct tgtgtcttta ctgattataa
    10981 ttacatttat agatattgac cactttataa taccagataa aatattggtt tttggagcta
    11041 tattttctat gatttttaac cttttattta aagaaattcc agttaaggat tcaattctag
    11101 gtgtttttat ttgtggagga agtgtctgga ttatagttct tttgattgaa tttgtaataa
    11161 aaaaagagtg tatgggtgga ggagatatca aactatttgc aatgctaggt ttttacatgg
    11221 gtgttaaaaa tggtcttttg acagccttac taagcgttta tgtgggagca gtctatggaa
    11281 tctgtgtaat aattataagt agaataaaag gaaaggaata taattctgtt ataccatatg
    11341 gaccttttat atctattgga gcattaataa caatcttatc tggaaagcaa ttacttgaac
    11401 tttatttttc tatggttata tagatgatag tcaagttaaa taaagtttta atttaaaaaa
    11461 atgaattaaa gtatttaata aggacaatat tattattaac cctattacta aagtgctaca
    11521 aactatggag tatataataa taatttcctt attttttgca catttgataa ataaatctct
    11581 atgtgttttt tgtgatagag aaaagaaaaa tttatataat acgtttttta tttacataat
    11641 aactgtagtt ttatctgtat taatatatct aaaatttggg attagtgtag aatgtgtaca
    11701 atatatggtt ttaattccat ttttaataat tatatccatt gtagattact acacaatata
    11761 tgtatatgat ataacaatag ttagtggtat aatagttcaa ggtataattt ttttggcctt
    11821 tttcaatata gttcaatttg attatataaa tcatatattt ggactattga taggctttat
    11881 tataccttat attattgcta aactaacaaa aggattaggc tatggagatg taggtctcta
    11941 tagcttatgt tgctttgcat tgggaaataa ttatgggttt tatgtgatag ttttatcatt
    12001 tattttagcg tttttgtttt ttattatttt atacataact aaaatattta aagatgtaga
    12061 tataagaaag caaattcctt ttacaccttt tatttccttg tcaacaattt taataatatt
    12121 tacagagtat aatatattta atttttatta taatttgtta tacaaattta tataacagga
    12181 ggtaagaaat gtacgtagta gtagggttag gtaatccagg aaagaagtat gagaagacta
    12241 gacataacgt tggatttgat gttatagata ttcttgctaa agaatacaat attagtgtta
    12301 ctaaaataaa gcataaagct ttgattggtg aagggagagt aggtactgaa aaagtattat
    12361 tagttaaacc acaaacttat atgaatttaa gtggagaaac attaatagat atatataaat
    12421 attataaggt tgatttaagt aatatagtag tagtatatga tgatatagat ttagaagttg
    12481 gaaagatacg tataagaaaa aaaggaagtg gaggaactca taatggcatg aaatctataa
    12541 ctaagtgcct aggttcaaat gactttccaa gagtgagagt aggtgtatct aaaccagaag
    12601 ctggtcaaga cttagcagac tttgtacttt ctagatttag aaaagaagaa tctgataaca
    12661 taaatgaagc attagaaaaa gcggcagatg ctattgactc aataataaga gaaaatatag
    12721 atatgtctat gaacaaatat aatggatagt gagagggaga tttaaatgaa tgatgttttt
    12781 ttatatcctt tgcaaaattc aaaagagtat aaagatataa taaactgtat aaaaaataca
    12841 aaaggctcat tattggttaa tggactttta ccagtacaaa aacctcatat atcgtattct
    12901 atatttaatg atttatcacg acaaatgatt tttataacga gtagtgattt agaagcaaag
    12961 aaagtatacg aagatttaag tttttatatg gaagataaag ttgaatattt aggttttcaa
    13021 gatatttatt tttatcactt ggatgcaaag gacagaaacg aagaagcgaa gaaattaaaa
    13081 gtattgttaa aactagcaaa taaagaaaaa atcattttag taacttctat tgaggcagtt
    13141 ttaagaaaat acataccaaa acaagttttg ctcgatagtg tatctcatta taaggtggga
    13201 gatagcttag acttagaaaa gttaactgag aaattagttt cattgggata tgaaagagtt
    13261 tcaaaaatag aggggtttgg tcaatttagt ataagaggtg gaattattga tgtattttca
    13321 cttgaatata caaatccaat tcgtatggaa ttatttgatg atgagataga ttccatacga
    13381 acttttgacg tatattcaca aaaatcaatt gataaattgc aacaattctc tataactcca
    13441 tctagggaat ttatttatcc cgaaaaaact acagatgcat tagttaaatt aaaaaaagaa
    13501 acaactaaaa atactgatga aggtacattt caaaatatag attatatatc aagtaagact
    13561 tattttgaag gtgtagagaa ctatatagat tatatttatc cagaagaaaa caaaagtata
    13621 tttacttatt tagcagatga tgcaatagta tttattaatg atataacgag acttaaagaa
    13681 agatgcgaaa attatataaa tgagtttaga gaaaattata aattaaatct tgaaagaggt
    13741 ctggctatta aaaaccaagg taaattgtta tatcactata cagatttaga gtacttagta
    13801 aaagacaagt ctgtggtatt aaactcgcta ttgccaaagt ctataaataa cttttctata
    13861 aaatctataa taaattttga aagtagagaa gttcctacat ttaatggaaa ggtagatttg
    13921 cttgtagaag aattaaatcg gctgaaatat aatggataca aaataatttt agcaacaaat
    13981 accttagaaa gagcaaataa attaggaaaa gatttattgg acaaaggctt agaaacgacc
    14041 atttctaaag atagagatat tgaaattaaa tcttcacaag ttataattgt acctgcacac
    14101 ataaacagtg gttttcagta taaatctata aagtttgttg ttataacaga taacgaaatg
    14161 ataggtgttt ataaaagagc gtctaagact agcaataaaa aagttaaaaa gggaaagaaa
    14221 atagaatcct ttttagattt aagtgttgga gactatgtag tccatgaaaa tagtggtgta
    14281 ggtagataca caggaattga acaaattaca gtcaatgcta ttaaaaaaga ttatatgaaa
    14341 atagtatatc aaggtggaga taatctatat gtgccaatag accagatgga caaagtacaa
    14401 aaatatattg gtgcagaagt agaaaaagtt aagttaaata agcttggaac aaatgagtgg
    14461 acgaaagcaa aagcaaaagt taaaaaagaa attgaagata tgacaaaaga cttgattgag
    14521 ctttatgcaa aacgtgaaaa aatacaaggg tataagtttt caaaagatac accttggcag
    14581 gcagagtttg aatcgttgtt tccatatcaa gaaacagaag accaattaaa ggctatagaa
    14641 gaaactaaaa aagatatgga atcaagtaag gtaatggata ggctagtatg tggagatgtt
    14701 ggttatggaa aaacagaggt agctattaga agtattttta aagcttgtat ggaacaaaaa
    14761 caagttgctg tattggttcc aacaacaata ttggcccaac aacattataa tacattcaaa
    14821 gagaggtttg aaaattatcc tttgagagta gaagttttaa gtaggtttaa aacaccaaaa
    14881 caacaaaaac agattattga agatgctaaa aaaggattag tagatatttt aattggaaca
    14941 catagaataa tatccaaaga tataaattta ccaaacttag gtcttgtagt cattgatgaa
    15001 gaacaaagat ttggagtaaa acataaagaa gctttaaaga aaattaaatc tacagtagat
    15061 gtacttacac tttcagcaac tcctatacca agaacattac atatgtcttt aagtggaata
    15121 agagacatga gtgttataga ggaacctcca caagaaagac atcctgtaat aacatatgtt
    15181 acagagagta aggaaagcgt aattcaagat gagatagaga gagaattaag tagaggtgga
    15241 caggtatttt ttgtatacaa tagagttgaa cacattgaag aaatggcaag tatgattcaa
    15301 aaactcgtac cagatgctag agtggctgtt gcacatggaa gaatgacttc taaatcatta
    15361 gaaaatataa tcctaggatt tttaaataaa gattatgatg tactagtctg tactacaata
    15421 attgaaactg gtatggacat atcaaatgca aatacaatga ttatatatga tgcagataaa
    15481 atgggattgg ctcaacttta ccaattgaga ggtagggttg gaagaagttc aagacaagga
    15541 tatgcatatc ttttgtatga aaaagataaa actttaagtg agatagcgga gaagagatta
    15601 aaagctatca gagaatttac agagtttggt tctggtttta aaatagcaat gagagacctt
    15661 gaaataagag gagcaggaaa tattttaggt tctcaacaac atggtcatat ggcagttata
    15721 ggttatgatt tgtacgttaa aatgcttaat gatgcaatta aaaaagtcaa aggtgaacct
    15781 attgtggagg aaattgatgt tgaaatagat ttaagtgtaa atgcatacat tccagataat
    15841 tatatcaaag atgagttgat taaaattgaa atgtataaga aaatagcttc tattgaaaac
    15901 aaagaagaca tgttggatat acaagaagaa cttgaggata gattctctga tatacctaaa
    15961 ccagtccaga cacttcttac aatagcttat ataaaatcgt tatgtaagat attaaaaata
    16021 gaaaagataa gacaattaaa agatgaaata ttattagttc caataacaaa gtatagaact
    16081 aaacagaaaa taggttataa tatagttaca gaattagaag aattattaga aaagatgtgc
    16141 aaggtaaaat aacttgtaaa tttaagaagg gatgttttaa atttgaaaaa ggtaataaca
    16201 ttggtaatag ctatgatttt ggtagtatct gtgactgctt gcagttctag taaaggagaa
    16261 actgtggcaa cagttgaagg tacaaagata tcatcagatg aatttaaaaa aactatagct
    16321 ctttataaag attctatgga acaaacttat ggaaaagata tatgggataa ggaagttgaa
    16381 aagggtgtaa aatacaagga caagtttaaa gatttaattc ttgaccaact tataactaca
    16441 gaagtaatat attctcaagc taaaaaagat aacttacttc ctaaaaaaga ggatgttgaa
    16501 aaaagcttta aagaattaaa agacgctatg ggtaaagatg aaaaatataa agaacaactt
    16561 aagaaattag gtatagatga tgagttttta agagaccaac aagagaaaga tttagcaatg
    16621 caaaactatc aaagtaactt tgctaaaaag actaaaatat cagatgaaga aatgaaaaaa
    16681 tactatgata cacataaaga tgaatttaaa aaagatgagg tagaagcttc tcatatttta
    16741 ttgaaaacag tagatgataa taacaagcca ctttcagata aggaaaaagc tgaagctaag
    16801 aaaaaggctg aagaggctct taaagaagtt aaatcaggtg aggattttgc aaaagtagca
    16861 aaaaaatatt cacaagatac ttcagcaagt gatggtggta aattaggatt cttttcaagg
    16921 ggtcaaatgg ttgctgaatt tgaagatgct gctttttcta tgaaaaaggg tgaagtatct
    16981 gatttagttg aaactcaata tggataccac ataattaaag tgacagacag aataaatgag
    17041 caaacatcat ttgaagatgc taaagaaact ataaaagacc aactgttgaa aaataaatat
    17101 caagaacaaa tagaaaaatt aactaaagaa gctaaggtag aaaaagatga aaaagtaata
    17161 aataaaatca caatctaaga cttaaaaaaa gagtaatatg cttaaaaaag ctatatctgg
    17221 ataagatata gctttttttg tatatttttc ttaacaatgg taaaaataaa cttaaagtaa
    17281 tttatggagg gattaaaaaa tatgaaagca acaggtatag ttagaagaat agatgatctt
    17341 ggaagggtag ttatcccaaa agagataaga aagacattaa gaattagaga aggagaccct
    17401 ttagagatat tcacagcaaa agatggagaa gtcatactta aaaaatactc tccaataggt
    17461 gaattaaatg aattttcgca agaatatgct gaaacattag ctgaagtact aggatatgga
    17521 gtgttgataa cagacttaga ttctataata gctgtgtcta agcttcctaa aaaagattat
    17581 aaagaaaaaa gtataagtga tgagttagaa gcaataataa gtgaaagaac agtaaaatat
    17641 tcaaaagata aaaacatttt accattattc aaggaagacc aaaaagaata tactagtcaa
    17701 gttatcatgc ctataattag tgcatctggt gactgtatag gttcaattgt agttgtttca
    17761 aaggataaag atacaattgc agaaagtgaa gaaaaatctt taaaaattgc tgctaacttt
    17821 ttaggaaaac aagttcaata attttatcat aaagaccttt tacatgaagg tcttttgaag
    17881 tttttgtgta aaatattgaa tatagcatta aaaatatggt atattaaagt ttgttatata
    17941 aacagtatgg aggttatgtt atgaacaata acaaaaataa agactccttt ctaaaaggag
    18001 cccttatact tgggctagca ggtgtaatcg taaaaataat gggtggattt tttaggattc
    18061 cccttggaaa catgattggt tcagaaggaa tgggatacta tcaggctgct tatcctgtgt
    18121 atactctatt tttaacatta gctactgctg gatttccaac agctgtggca aaattggttt
    18181 cggaaaaagt tgcaattgga aattttaaag gtgcaaataa gatatttaag gtatcacata
    18241 cagttttatt cattaccggt ataatatcat tttgtatatt attttttgga gccgactaca
    18301 tagtaacaaa cgtaatgaaa aatccaggtg cgttatactc tatgaaagcc attgctccag
    18361 cattactatt tgttcctgca atgtctgcat atagaggata ttttcaaggg agacaagata
    18421 tgactaagat tgctgtgtct caagttgcag agcaattttt tagagttgta ttaggtctta
    18481 ctttggctta cttgcttatg aagagtctag gtcagacttt tggtgcagca ggtgctatat
    18541 caggggctac aataggttca atagcatcta tgctttattt ggtgtttgct tatatgctag
    18601 ggagaaaaga gcgtagagca gagatagatg caagccaaag atttaaagat gaaagagtgt
    18661 cttatatttt taaaaagctt ttaactgttg ccattcctat aacaatagga gcatcagtaa
    18721 tgccacttgt aaatatgata gataatgtta ttgtaataag aagacttatg gaagcaggct
    18781 ttacatacaa agtggcaaat tccatgtttg gtcaattaac aggtatggca atggccacaa
    18841 taaatttacc agcagtcata acaactgcaa tgagtatgag cttagttcca gcaatttcta
    18901 aagcatatgc attaggaaat aagtcaaaag ctagaaaaga tactaagagt gcagttaagg
    18961 taactttact tatagtattg ccttgtgcgt ttggtatggc ttctttagca ataccaataa
    19021 tgggtctttt atttccacat gaaccatctt ctgtaggaac tattttgttt actcttactc
    19081 catgtgtatt atttttagga ttaatacaga cattgactgg tataattcag ggaatgggaa
    19141 aaccaatagt acctgtaatt gctttatgtg ttggtatgct atgtaaaatt gtaataagtt
    19201 atactttaac aggtatacca gatattaatg tactgggttc agcatttggt acagttacag
    19261 catactttgt ggcagccatg attaacttac tatatgttaa aaaacacatg aatgtaaact
    19321 tttctaaaaa agaatttata ataaagccat ttataacagt tatgacaatg tttataatgg
    19381 ttaagttgag ttatggtgct ttagttggat tcttaggaaa cagcatatct acaataattg
    19441 caatatgtgt tggtggaatt gtatatgtag tagtaatact tggaataggt ggaattaaaa
    19501 aagaagagat tctaactatg ccaaaaggtg ataaattata taaattgtta aaaaaagtta
    19561 aacttataag ataatatagg aatatatatg caaataaagg aaatactata caaatagtaa
    19621 atctccttat ttgtataata atataaaaat aaagtgaaat ttgctaatag gagtgatgtt
    19681 atgggtaaga taagtattgt aggtcttgga ccaggagact attctcttat aagccaaggt
    19741 gtgttagaat ctttaataaa tagccctaaa gtttttttta gaacagagaa acatccaata
    19801 attgataaac ttaaagaaac tatacaatac acttctttag actactttta tgaaaaagag
    19861 gaaaattttg ataatgttta tttgaaaata tctgaattta tcatagaaga atcagcaaag
    19921 ggtgacctag tgtatgctgt tcctggtcat ccaagagttg cagagaagac tgtaagtata
    19981 atagagagat tagccaatga aaagcacata gcagtagaaa tattgccttc tatgagcttt
    20041 gtagacgcta tgtttaacta tttggctata gacccatctg agggatttaa actggtagat
    20101 gcttttgaaa tagaaaattc atatatagac ttggatacaa gtatgataat aactcaggta
    20161 tatgataaat ttatagcatc taatgtaaag ttaaaactta tggaacatta tgactataat
    20221 caagaagtat gtatagtaaa tggagctggt gtcaaagatt tagaaagtaa aaagtttata
    20281 aagctttgtg aattagacag aaatgaaaac ttttttgact atttaactag cttatatata
    20341 cccaaaagtt ctaaaaagat gtataataca gtacatgact tagaaataat agttaataca
    20401 cttagaagtc catctggatg tgaatgggat aaaaaacaaa cacatcaatc attaaaaaat
    20461 tctgttatag aggaagcata tgaactttgt aatgcaattg ataataatga tatagatgaa
    20521 atggtagaag agttaggtga tgttctttta caggttatat tccattgtca gataggaaat
    20581 gaggagggct actttgattt aaaagaagta gtaaatggca tatgtaaaaa attaattcat
    20641 agacatcctc atatatttag tggtgcaaaa ttagatatgt ctaagtttga gaaaacttgg
    20701 gaagaattaa agaaagaaga gaaaggcgaa agtactataa cagatggcct tagaaggata
    20761 ccaaaacatt taccagctct tatgaaggct agtaaagttc aacataaagc agcacttgtc
    20821 ggctttgatt gggacaatgt agaagatgta tttaaaaaaa ttgaagaaga atatcaggaa
    20881 ttacttgacg aacacagggt cgggaatata aaatacataa aggaagagct aggcgattta
    20941 ttattttcga tagtaaatct agctagattt ttagacatag actcaggaga agccttgaac
    21001 tgtactacag aaaaattcat aaatagattt gactttatag aaaaaagtgc aatgaacttg
    21061 aataaaaaat tagaagacat gacacttgaa gagatggatg agttttggaa tcaggctaaa
    21121 aaacaataaa attgtggaag gaaatttgtc aaaaatctag aacttttatc acaaagatag
    21181 aagtctatat tataaacatt tttaggaggt aaatatcgtg aataaagctg aattagtatc
    21241 aaagatggca gaaaaaagtg gattaacaaa gaaggaagca gaagctgcgt taaacgcatt
    21301 tatgagttct gttcaagatg cactagtaaa taatgaaaaa gttcaattag ttggatttgg
    21361 aacatttgag acaagagaaa gagctgctag acaaggaaga aatccaagag atccagagca
    21421 agttatagat ataccagctt ctaaagcacc agttttcaaa gctggaaaag gattaaagga
    21481 tataataaat ggataattag aaatcttgtg aaaaagtgtg gataaatttg ccacactttt
    21541 tctatagaac caaggaaggt gataattatg agattagata aatttttaaa agtatctagg
    21601 attataaaaa gaagaactgt tgctaaagat gcatgtgata aagggattgt cactataaat
    21661 ggaaaagttg ctaaatcttc ttcagaggtg aatattggag atacattaga aatacaattt
    21721 ggagagaaga agatgaagtt taagataaat gaagtaaaag agcatgtatt aaaaaatgat
    21781 gcaaaagaaa tgtacaaaat tttagaataa aattaatagt cctctagtat attaatagta
    21841 gtattattga aatatttact ggggggatat tatggaacag aacataactt tgaaagatag
    21901 atcaaagctg gttatttcgg gagtcgagca catatactct tttaatgaca aaagagtaga
    21961 gctcaaaact tctgtaggcg agatggtaat tgaaggcgaa aacctagata tgagcaagct
    22021 aagtatagac gaaaatataa ttagcataga tggaactata aattctatgg tatatgcaaa
    22081 gcctaaaaag ccacaagaga gtttcttgaa aaaggtattt agatagatga taccttttac
    22141 tcaagatgta agcatttttt atgctactat ttatggtgga atactcatag gggtgttgtt
    22201 tgatttttat agagggctaa gagggaattt taaattcatc aattattttg ctataatctt
    22261 tgatgtattg ttttggtttc tagctacggt tataatattc gtaacaataa accttacaga
    22321 attttttgat ttaagatatt atcattttgt ggctttgttt attggtttta ttttatacta
    22381 taacaccata agtaaaattg ttttatcaat tatcaataaa ataattcgtt ttgtgagaaa
    22441 ttcttttaaa aaagttacac attacatagt cagtttttta aataatttgt attacgttat
    22501 aatctattca ctacacctac tatttgatat tatattttat ataccaaaca tatttatagc
    22561 aacaagaaaa agtataaaaa gaaaatcaaa taaaaaatta aagaacaaga aaaaatcaaa
    22621 acctaagaag aaaaaaaata aaactaaaaa aagggtgtag gaaaaatgaa cttaagaaaa
    22681 aaattttcag gacagattat tgttatcagt ttatttttag gaatatcaat ttttagtatg
    22741 atgacaggat ttgtatttga gtatacaaaa gctaaggaat ataaaaaaga aatagcaagt
    22801 ctaaataaac aattaaaaaa gactgaaata caaataaaca gcttaaagaa agatgaaaaa
    22861 tcatatgaag gagacttaga ggatatagca agaaaaagat tgaatatggt aaaaccaaat
    22921 gaaacagttt atgtagacat aaatcgttga ttaaaacttg ctaataagtt tacagatgta
    22981 ttgattttaa aatttaagat ttaataaaaa agtcattcag ttaaggatgg ctttttgttt
    23041 ttattgagga gggaatttca taatgaaagt aggagctata gatattggga caaactcaat
    23101 gagattattg atagcagatt atagagatgg gaattttata aacagaaaaa aatttgttaa
    23161 tacaactaga attggttatg gtgtggatag taaaggatat ataaaagaag atgctatcaa
    23221 aagaaatata aaagcattaa aagaattttc tgataagtgt aatgatgaaa agtgtgaaaa
    23281 aatttattgt atggggacat ctgctcttag agacagtaaa aatggagatt actttgttaa
    23341 attagctaaa gaagaaactg gaatagatgt tgatatagtt acgggaatag aagaatcaaa
    23401 tttgggcttt aagggtgttt tagaaggaat acaaggtgat gatactatta ttgttataga
    23461 tattggagga ggttcaacag aatttattgt aggtgattca gaaggaatta aatttaataa
    23521 aagtgagaat gtaggtgctc ttaggatgac agagaggttt ttgaagcacg atattattga
    23581 agatgaagag tttgaagcta tgagttcatt tatatatgat gagattgaca aaacgttaaa
    23641 tcatataaaa gacagaggat taaaaactct tataggtata ggaggtacga ttacatcatt
    23701 atcagcaatg aatcaagaat tagagattta ctctatggaa aaaatacata atagtaaaat
    23761 acacaaaaaa gaaatagaag aaattctaca aaaattaata aagatgacat taagtgataa
    23821 aaagaactta aaagggctac aagaaaaacg tgcggatatt ataactgctg gtgtaaaaat
    23881 attgaatata ataatggaaa aattagaaat agaagaaata accgtaagtg aatacgataa
    23941 tttggaaggc ttaatatgtc aaaagtcaaa aaaaatgtat taatattttt tgatttttaa
    24001 aaccgattat gacaaaaaaa attactcctc tttgttacaa ttcttaatat aaacaaaaga
    24061 ggaggttttt ttatgcaaag aagtgtagca gttgtgaaga ataagactgt aaaaccaaat
    24121 gtcataaaat taataagcgg atatattgat tttactacgg cggcattcgc tgtgatggga
    24181 tttttactta gtaggtctat tatagtagac tctgtagctc ctttaggaat tgcctttttt
    24241 atatgtgcat caaagattga taagtacaag atacctgtat tcatatcaac attattagga
    24301 atattgttat catctaatga ttttggaaat gtagcaaaat atgctgtgtg ccttacaata
    24361 tttatggcta tgaataaaaa attaaaatta tcagattcaa ttgctaaggt tgcatttata
    24421 ggtgcaataa tactacttcc aatctccatg ggacaagctt tcttttcaaa taggtatgta
    24481 tatggtcttc ttatagcagg agtagaagca ataataatgt ttatatctat atatgtgttt
    24541 tcttatggga taggtttaat ggccaatgtc aacaatagga tgtctgtacg gacagaagaa
    24601 gccatatcaa taagtttact gctaaccttt agcataatgg gaataggaga tatatctctg
    24661 tttggaatat caataaggac cattttatcg accgtattga tattgctgac ctcaatagta
    24721 ggtggagcta ccatgggagc atctagtggt gtaattattg gtgtagcatc cattttaaat
    24781 aatatgacat cagctgttta tatggggata tattcttttt caggattaat aagtggagca
    24841 tttaataaaa taaataaata tttctgtata ctaggatata ttctaagttg gacaataata
    24901 tacctatata cttctggtat tacttctaat atgatgcaat taagagatat actattaggt
    24961 tgtttgatag tactagtatt gccagagaga ttatttaata agattgaaaa attaataaaa
    25021 agtaatgtgg cttcaaatga aatcgtatac gattatatta tgagaagcaa aaacctaact
    25081 aatagcagat tgaacagtat ttataaaact tatgatgacc ttgcagatac atttgataag
    25141 ataagagaaa aagacaaagt gctagaccaa agagacatag ctaatgtaat agatatgatt
    25201 cataatgatg aatgtaaaag ttgtagtatg agaagaatgt gctgggagtc aagatttaat
    25261 catacataca ctatgatata taacattctt gaaaaaattg aggagaaagg ggagttaagt
    25321 ctaaacgata tacctaaaaa ttttagaaag gaatgtatga aaccagagtc aatagtgaaa
    25381 atatcaaatc actattacaa aatgtttgta ttagattatg attggagtgt gaagttttca
    25441 gaaagtagaa agttaatagc aaatcagata agaagtattt caaaatccat taaatcctta
    25501 tctcaagatt tagaaggcga cataatgcta gatatagaaa aggaaaaaaa tatatatgaa
    25561 cagttagaaa gatatgatat aacagttgat aaagtaagtt acttaacaaa aagtaacagt
    25621 gagtttgaaa tatcaataga aaagaaaact tgccatgatg gatgtatgtg tgaagataaa
    25681 attgtaaata taatttcaga cctagtaggg gaaaatatgt ctgttagaaa aataggatgt
    25741 cactgtttag gaggtaaatg caaggcaaca ttcgttaaat ctcaaaaata taaggctgtt
    25801 acagaagttt cagcaatgtc aagggatggg catatccttt gtggagacaa ttacacatac
    25861 atggagataa atgatggtaa atacatgatg gctataagtg atggtatggg aaagggtaaa
    25921 aaagcatatg aagaatcttc tgccactatt gatatactgg aaaaaatgat agatgcaaaa
    25981 attaaagatg agatagtcat tgatactata aacaatatgt tgttgttaaa atcttcagaa
    26041 gagatgtttt caactctgga tttgggaatt ttagatttaa aacgaggttg tttagaaact
    26101 ataaagatgg gagcgtgttc tacttatata aaaagagagg atggagaagt tgacttaata
    26161 tcatcatcat cattacctgt gggtatatta tcagatgtaa aaattgatag aaaaaatgta
    26221 aaagttaagg aaggagacta tgttataatg gtttctgatg gaattgtaga tgcaggaaga
    26281 aataacaatt taggagacaa ttggttaata tacttcttaa agaatataga gactacaaat
    26341 ccaaaggaaa tttcaaattt aatattggat agagcattag agttacaagc gttacaaatc
    26401 gaagatgaca tgactgtttt agttacgaaa atatgtacaa aataaggttt acaagattat
    26461 gtgtataaga tgtggataac ttgtgtataa tatgttaata gacttttgaa aaaagtatag
    26521 ttattaacaa aaataacata taaatcaaca aaatttgttg ataagtatgg cagaaatgtt
    26581 aataaaatgt aaaaaaagaa aaaacaaaag ctattatcaa aagagattca tgttgaaata
    26641 actaaatttc taagtaaaga aaacctgtga atatgttgat tattatgtgg ataactatta
    26701 attcgatatt tatttatata gtatgaataa ttgaataata tatctaaaaa aagccatcct
    26761 attattaagt aaacttattg aaaggatggc tttttatgaa tagaaaattg tgcaaagcaa
    26821 taataccttt atttatttct attttttgca tgttaataat aagtataaaa gctgaccaag
    26881 gatatcaaaa gaataatgac agagcaatta ctgtcatgaa taataatgat actgaagatg
    26941 atgctgaaaa aaaacaagaa atagactatg aaaaaacatg ttggaatttg caatctttgt
    27001 ttaaaagtga tgaccaatgg aaaaaagaac ttaagagttt tgataaagat actaaagagt
    27061 tgaaaaatta cataggtaaa gtaacaaaat cgccaacaca tttgtctttt gccttgaaca
    27121 ttaaagaaaa acttgatata agactaaata aactttctgc ctatgtaaaa ttaaaacaag
    27181 atactaataa aaattcgtat aagtacttag atatgaatga tagtatgagt aaatcctatg
    27241 ggaattatat ggggatttgt tcagatttag agttagaaat actaaaatta tcaaacaaag
    27301 attacaagaa aattatttca aataaaaata taaataggaa atatggtgca tatttaagag
    27361 atataagaag gaataaagta cattatttgg atgataaatc agaagatatt ctaagcaaag
    27421 tatcttcaat aagtagtctt ccaagtcaag tgtatgaatt atttagaaat atggatagaa
    27481 aaactaattt aacaccagct caatatgcga gtgaattaga gtcatcagat agagaaaata
    27541 gaaaaaaagc ttatcaagat gaacttataa cttacaatga taatataaat acaattgcag
    27601 gtttaattac tggtcaagtt aaaaagaata ttttttattc tcaggaaaga ggttatgagt
    27661 cttctttaga aatgtacctg gaagctgata atgtagatga aaaagtatac aataatttga
    27721 taaggactgt aaatagtaat atggacagtc tacacaaata tatagattta agaaaaaaag
    27781 tattaaaatt agataaggtt cattcatatg atatgtttgt gcctatggtt aaggctgttg
    27841 ataataatat taattatgaa aaggcacaaa gtatagtata ttcagcgttg tcacctttag
    27901 gaaaagaata caatgatgta gtatataaag ctttcaatga aaaatggata gatgtttatt
    27961 cacatgaaaa taaagtaagt ggtggatact gcttatctgt ttatgaaaat catccatata
    28021 tacttttaaa ttataataat tcattaggtt cagtttcgac tttatcacat gagcttggac
    28081 atgctgtata tgaatactta agttctaaga atcagaatta ttttaattca aatccgtcta
    28141 tatttactca cgaagttgca tctacaacga atgaagcatt actttacgag atgcttataa
    28201 aagaagctaa aggcaatgat gaaaaagctt attatataac acagtattta gacttgataa
    28261 aagatactct ttatacacaa actatgtatg ctgaatttga aaaaactata cacgagttag
    28321 tagaaaaagg aaaaagtgta aacgcattag tattaaatga tgtttgggga caacttctta
    28381 aaaaatatta tggacaaagc tatgaattag accaattatc taaggtcgga tgggctagaa
    28441 tcccacattt ttataatagt ttctatgtct ataaatatgc tacaggttgt agtgcaggag
    28501 taagttttgc ccaagacatc ctaaagaatg gttcagataa ctatatgagc tttcttaaaa
    28561 agggaagttc agattatcca ataaacttgt taaaatcaag tggcataaat ttaactaata
    28621 caaaacccat agaaaataca atcaaaaaat ttgatagttt agtagcagaa cttgaaaagc
    28681 tattataaac attattgtaa tccacatggt aaatatatcg aattaatgat aaaatatact
    28741 aaaagtttaa agtcctatca agataggact ttttatttat taattagtgg gagatgattc
    28801 tatgcaagtt aaagttaaaa aggctgtaat accagcagca ggtttaggaa ctagattttt
    28861 gccagctaca aaggcacaac caaaagagat gttaccaata gttgataagc caactctaca
    28921 atacataata gaagaagctg tggcctcagg aatagaggaa atactaataa taacaggtag
    28981 aaataaaaaa tctattgagg accactttga taaatctgta gaattggaac ttgatttaga
    29041 gaaaaaaggt aagaaagaat tgttggaggt agtacaaaac atatctaaca tgattaacat
    29101 acattatata agacaaaaag agccaaaagg gttgggagat gcaatttatt gtgcgaggca
    29161 ttttataggt gatgagccgt ttgcagtaat gttaggggat gatattgtag ataatgatgt
    29221 accttgtcta aaacaactaa cagatgcata tgaggaatat agaactacaa tattgggtgt
    29281 tcaaaaggta aatcaagaag atactaataa atatgggata atagaagcaa aaaatataga
    29341 aggtagagtt tacaaggtaa aagacatggt agaaaagcca gagtcaggaa aagctccttc
    29401 aaatattgct attttaggta gatatataat aactcctgaa atctttgata ttcttaagga
    29461 tttaccacca ggtaaaggtg gagaggtaca acttacagat gctttaaaaa tattatctaa
    29521 gaaagaagct atgtatgcat acaattttga aggcaagaga tatgatgttg gggacaagct
    29581 aggattttta gaagctactg ttgactttgc tctaaagaaa gaagacctta aggaagattt
    29641 tataaaatat ttgaaacatg tatgtagtga atttgataaa aataacaatg tgttatacaa
    29701 taatacagat gaaaaaatag atttaattga agtcaatgaa gctagagaac ttgaaattca
    29761 aaaataatta atgaaaggtg atgtatggtg gcaaaatcaa gtcagaaaag aattaataga
    29821 ataatagctg ttgggattgt gggaatcctc gtaataacga gtataacagc tgttttgagt
    29881 atgattgcaa ctatgtaata agataaatga cagcagttga tatacataaa tataaaaacc
    29941 atatataact tgatgtagtc aaaaacttac aatattttag ttatatatgg tttttagaat
    30001 ttataaatgt aaaaaaaaat ttttataata gttgcatatt ttaaaggaaa atattattat
    30061 aagatataaa tatagtgata aatttataaa aacgtgctaa gattggactt gaaatacagt
    30121 aatatagtag gtaataatgt tttaaatttt atttattgac cataatatat aaataatgat
    30181 attattagac ttaactgttt aaataaaact tttctttttc atgccttcaa tttccaaaat
    30241 aagtatgtat ataaatgaat atatagatga ggaggatttt tttgatttta gatgataaag
    30301 tattagaagg attaggtagt aagaccttag ttgagctacg agagatagct aaagaattaa
    30361 aaataaaatc aattactaca tataagaaaa atgagttaat tgagattata aattccaaaa
    30421 gtaaaaatga ggaaaaagta aacgaagaga ctaaagaaaa aaatgaagat attaaagaag
    30481 acataaagaa tagaaaagaa aatatagagc gtgaaagtat agaaaaagaa gttgaatata
    30541 agagccctac caaatcatac aacaagaaag aaatagatac ccaaaatatt tcccaaaacc
    30601 aagaacatag aaatcaaaga aatgactata ataagacaac gagtgtaaat gatagtaata
    30661 agtcatcaaa tatatctaat agaatggtca gaaataataa taaaaactat tatatgccta
    30721 aacaagttga tgaatctaag atagtagatg aatttaacac atcaaaagag gatgaagtag
    30781 taggtgtact tgaaattctg ccagatggat ttggtttttt aagaggttca aattatttgt
    30841 ctactgaagg agatgtttac gtttcaccat ctcaaataag aagatttaat atgaaaacag
    30901 gagacaagat taagggaata actagacatc caaaaagtgg agagaaattt agagcacttt
    30961 tatatgttca aaaaataaat gatgaaaacc ctgacacagc tatacaaaga aatgcgtttg
    31021 agacattaac accaatattt ccagaagaac gacttacact ggaaactaat agaaatgaaa
    31081 tagctacaag aattatagat ttaatttcac ctataggaaa aggacaaaga ggattgatag
    31141 tagcacctcc aaaagctggg aaaacagttc ttttaaaaag cgttgcaaat agcatagcta
    31201 aaaatcatcc aaatgttgaa cttatagtac tccttataga tgaaagacca gaggaagtta
    31261 ctgatatgaa agaatctatt gaaggagacg ttatatattc tacttttgac caagtatcaa
    31321 gtcatcatgt caaggttgca gagatggtgt taaatagagc tcaaagatta gttgaacatg
    31381 gtaaagatgt agtaatttta ctagatagta taactagatt ggcaagggca tataatctta
    31441 caatatctcc tacaggtaga actttatctg gaggtattga ccctggtgca cttcatggac
    31501 ctaaaaagtt ctttggtgca gcaaggaata ttagacaggg tggttctttg acaatattag
    31561 gaacagcatt ggttgaaact ggttctagga tggatgatgt aatatttgag gaatttaaag
    31621 gtacaggaaa tatggagcta catttagata gaaaattagc agaaaaaaga atattccctg
    31681 caatagatat ttacaagtct ggtacaagaa gagatgattt attgcttgat gatgaagaaa
    31741 agactgctct ttggagatta agaagagaaa tgagtaataa ttcagtaatg gaaattacag
    31801 ataaagtcat tgaacttata aagagaacta aggacaataa agagtttgta aaatcaataa
    31861 agaatttgta aaatcaataa tacaaatgca aatatggatt atataaacta atagaaaaat
    31921 cacttgaatg gcataataag ttgtgctata ataatgtagt tgataatgta aaaagaaagt
    31981 taatataatt aattttttta aataaagaga agaggtgact ataatgcaaa aggaaataca
    32041 accaaaatac aatccagttg aagtgcgttg tgcttgtggg aacacatttg ttgctggttc
    32101 aactaaggac gaaataaaag ttgaaatatg ttcagagtgc catccattct atacaggaaa
    32161 acaaaagaac atagaaaaag gtggaagaat cgacaaattc aagaaaagat tcaaaatgga
    32221 ctaattttta taaaagtgga gttggaggaa gccaactcct attttattac aaaaaaacat
    32281 tcatattcta ctttaaattg tctttttgta ttataataat aaatatgtaa aattgagtat
    32341 tctaaataca ataaaatgag gtgagtggat ggcaaaacaa gctgtaggtg ggcaagctgt
    32401 tattgaaggt gtaatgatgc agtcaaaaga caaaagagcg gtagctgtta gaaaaagtga
    32461 cggagaaata gcactaaaag aagatagaat aaaaagttgg gtaagagata agaatataga
    32521 taagatacct tttgttagag gttcttttgt tatgatagat acaatgattc aaggcataaa
    32581 aagcttaaac ttttcttcag agttttttat ggaagaagct gaagaggata aatttgattt
    32641 atttataaag aaaattttta aagataaggc aaatgatata attataatat tttcattggt
    32701 catagcaatg ttactttctg ctggattatt catttttata cctacattgg taggaggagc
    32761 tttttctaaa gtaatgccta atgattttat gcttaattta atagaaggaa tcataaggat
    32821 agctatatta tttgcatata tagtattgat ttcacgaagt aaggatatag aaagagtctt
    32881 tcaatatcat ggagcagaac ataaatctat atattgctat gaaaatgatt tagaacttac
    32941 agtagagaat gctagaaaat ttaaaagatt acatcctaga tgtggtacaa atttcctatt
    33001 tatagttatg gctgtatcaa taatattgtt tgcatttttt ggttggccaa atccaattct
    33061 tagaatattt atgagaatta tatgtgtgcc tatagtagca ggtttatctt atgaggtaat
    33121 tagagttctt gggaaatatg ataatggatt tactaaaatt attgcatatc caggaatgat
    33181 gttgcaatat tttacaacaa aagagcctga tgatgagcaa ttagaagttg ctttggaagc
    33241 attgaaagca gtagtagatt agagaaaagg atatttgtta tgacgataaa agatataatt
    33301 attaaatatt cagataaact aaaagatata agtgatactc caagattaga tacagagtta
    33361 ttgttacaaa aaacattggg tgttgataga ttatatattc acttaaatct gaataaagag
    33421 ctaactgaag aacaaaaaac aaaatttatg ggttttgcag aagaaagatt aaatggaaga
    33481 ccaatagcat atatagttga aaatagggag tttatgggat tagatttttt tgttaaggaa
    33541 ggcgtattga ttccaagacc agatacagaa actttagtag aagaaataat tgaaatatgt
    33601 agagaaaaaa aagatgtaag tatattagat ataggaactg gttctggagc tattacaata
    33661 agtttagcaa agtacattga aaattctaaa atcatgtctt ttgatatatc agagacagca
    33721 ttagaaatag ctaaaaaaaa tgctattata aatgaagttg gtgaaaaaat aaagtatatt
    33781 aattctgatt tatttacagc aataagtgat agtaacataa aattcgatat catagtttca
    33841 aatccaccat atattaaaaa acaagatata gaaactctgc acaaacaagt taaagattat
    33901 gaaccatata atgctcttga aggtggagaa gatggattag acttttatag aagaataaca
    33961 gaacaaggta agaaatattt aaataagtgt ggaatattag catatgaagt tggtcataat
    34021 caagctgaag atgtaataaa tattatgaaa agtaatggat ataaaaaaat atacacaaag
    34081 aaggatattc aaggcattga tagagttgtt ataggttaca atatatgata taatcaacaa
    34141 ttgatatgtt acatttaatc gaggtgaaag tatgttaaaa aaattagagg tattggaaga
    34201 tacatataaa gacttaagtg aaaaaatagg tgatccggat gtaataaatg atcaaaaagt
    34261 ttggcagaaa tatataaaag aacatgctga cttggaacct attgttatga aatacagaga
    34321 gtataaaagc gttttagata gtataaaaga atcaaaagaa attttacaag aagagtcaga
    34381 tgaagaatta agagaattag ctaaaatgga gttagctgag atggaagaaa aagtagcacc
    34441 tctggaggaa gaaataaaaa tactattatt gcctaaagac cctaatgatg ataagaacgt
    34501 tatagttgaa attagaggtg gagctggagg agatgaagca gctctatttg ctggtgattt
    34561 atttagaatg tactcaaggt atgctgaaag aagaagatgg aaaatagaat tattgagtgc
    34621 aagtgatact ggagttggtg gatataaaga agtatctttt atgataaaag gtaaaggtgc
    34681 ttattcaaga ttaaagtatg aatctggagt tcatagagtt caaagaatac catcaactga
    34741 atctggtgga agaatacaca catcaacttc tactgttgca gtattaccag aagttgaaga
    34801 tgtagaggtt gaaataaatc caaatgattt aagaatagat gttttccgtt cttcaggtaa
    34861 tggaggacaa agtgtcaata ctactgactc tgctgttagg gtaacacata tacctacagg
    34921 tgaagtagta tcttgtcaag atggaaaatc acaattaaaa aataaagagc aagctttaaa
    34981 aatattaaaa gctagacttt acgataaagc attggcagaa caacacaaag atatagcagc
    35041 agaaagaaaa agccaagtcg gaacaggaga caggtcagaa agaataagaa catataattt
    35101 ccctcaaggt agaataagtg accatagaat aaatttaact ttatataagt tagatgcatt
    35161 cttagatgga gatatagatg aaatgataga tgctttaatt actgttgacc aaacagaaaa
    35221 aatgactgca atataagtaa tgttttaaag aagctgtttc ttcctagaaa ttattttcta
    35281 ttttgaaaca gctattttgc attttaggtc atgttatcat attctattgt atataattgt
    35341 tatatacaag tataggaggg ttatatgata ctaaaagtaa caataattgg gcttttggca
    35401 ggtgttatag ggacaggatt aggaggagta atatcagcca tttttaaaag agaggtcgat
    35461 aaatatctaa gtttttttat gggattatct ggtggaataa tgttggctgt agtggtgttt
    35521 gatttaatga aagaatccat ggacaaaatg gggataatca atacagttat atttacgttt
    35581 gttggagctc tcataactat gtacataaaa acaaaattag atgtgtctgg aaatatggca
    35641 tctggatatc ttatattcat aagtatatta ctacataatt taccagaagg actagccatt
    35701 ggttcttctt ttatgtcaac tgaaagttta ggtattacac ttgctatagt tataggtcta
    35761 cataatatac cagaagggtt agctatggca ttaggcttag tttgcaataa aatgaaacta
    35821 agtaaagtta ttttgtttac tgtaatagct ggattaccta tgggtctagg aagtttttta
    35881 ggtgtttatt ttggaggagt atttacttct cttataggag tatttcttgc tacagcagga
    35941 ggtactatga tgtatgttgt attggaagaa atttttccac attcaaaaag tgtgtattgt
    36001 ataattggtt tcttgttagg tacaatgatt gtaaattata tataaaatta aaatgaaagc
    36061 aggcatacaa aagatgaaaa ctattataag taatattgat ataaataata ttgattatga
    36121 ggaaataaaa atacaagcaa aacttcttag agaaggtaaa acagttattt ttccaactga
    36181 gacagtatat gggttgggtg caaatgcatt agatgaaaat gcagtaaaga agatttatga
    36241 agctaaagga agacctagtg acaatccatt gatagtccat atatatgaaa aagaagaagt
    36301 atatgactta gccaaagata taagtgataa agctaaactt gttatagaaa agctttggcc
    36361 aggacctata acaattatat taaataaaaa agatataatt ccatacaaaa ctagtggagg
    36421 gcttgataca gttgcaataa ggatgccttc taatgttatt gccagggcaa taataaaaga
    36481 agctggtata cctattgcag ctccttctgc aaatatctca ggtcgcccat ctccaacaaa
    36541 agcaaaacat gtatatgaag agatgaatgg aagagtggat ggaatagttt taggaggaga
    36601 cagtaatttt ggattggagt caactgtatt ggatttaaca gaagagactc ctatgatact
    36661 gagaccagga agtataacta aagaagtttt agaggaactt cttggagaag taaagcttga
    36721 cccatcatta agtaaaaaag aagataatca aaaagctaaa gctcctggaa tgaaatataa
    36781 acattattct ccaaatgcag atgtatatat aatatctgga aaaagagaaa atgtagcaac
    36841 aaagattaat gatatgataa aatctaatag agacaaaggt ctaaagacag gtgtaatgtg
    36901 tattagtaga aaccgagagt tttatgatgg agaagtaatc gatttaggaa actctcttga
    36961 agaagttgca agtaatctgt ttgatgctct tatagaaatg gacaaaaagg gtgttgatgt
    37021 catatattca gaagagttcc ctaagactgg agttggacaa gcaattatga atagactact
    37081 gaagtcagca ggatacaaag tgataaaggc ttaagaggga gttgtaaaat atgaatatac
    37141 ttattgtgtg tacaggtaat acttgtagaa gtcctatggc tgaggctata ctaaggaaag
    37201 caattaaaga atctggaaga agtattgaag aatattctat atcatcagca ggtatatcta
    37261 cagcaaatgg aatgggtgct tctgaaaatt ctatagaagt actaaaagaa atcggtatag
    37321 acttatcaaa tcatagaagt aaagtaatta ctaaaaaatt aattgatgaa tcagatataa
    37381 tactaacaat gactaaatct cataaagaaa ttttagtaca agcagttcca aaatgcaaag
    37441 aaaaagtgta tacttttaaa gggtttgcaa ataaaaatga ggaagatata tctgacccat
    37501 ttggtggaaa tttggatatt tatagaagca ctatgagaga aataatgtat tctgtcaacg
    37561 aaatagtaaa aaaaatatga aacggaggat aaacaaatga agataggatt aggctgtgac
    37621 catggaggat ataatttaaa aaaagaaata ataagctatt tagaaagtaa aggcatagaa
    37681 tgtgtagatt atggaactaa taatgctaca gattctgttg attatcctgt ttatggagaa
    37741 atagttgcaa attcagttat aaataaagaa gtagattatg gaatattatg ttgtggtaca
    37801 gggataggaa tatctttagc tgctaataag gttcctggga taagatgtgc agttgtatca
    37861 gatgtatttt ctgcaaaaat gtcaaaagca cataatgatg caaatatgtt atcattaggc
    37921 gaaagagttt taggaaaagg tctagcactt gagatagttg aagcatggat taatacggat
    37981 tttgaaggtg atagacatgc tagaagagta aacatgatta aatcaataga agaaaaacat
    38041 aataaataat aaaaattaca gttttatcag gaggaattat caaaatgagc aaagtggtag
    38101 agacaaacca tccattaata caacataaat taactttaat gagagataaa aatactggtt
    38161 ctaaagactt tagagagctt ttaactgaaa ttgcaatgtt aatggggtat gaaataacaa
    38221 aagatatacc attaaaagat gtagaaatag aaactcctat tcaaaaaaca tcatctaaag
    38281 tagttgcagg taaaaaatta gcaataattc caattttaag agcaggtctt ggaatggtag
    38341 atggattagt gagcttaatg ccagcagcaa aagttggaca tgtaggacta tatagagacc
    38401 cagaaacatt aaaaccagtt gaatattatt gtaagcttcc tcaagacata ggagaaagag
    38461 atataatagt agttgaccct atgcttgcaa cagggggttc tgctgtagca gctatagatt
    38521 tacttaagtc taaaggagct aaaagtataa aattagctaa tttagtagct gcaccagaag
    38581 gtatagcaga agtgcaaaaa tatcatgatg atgttgatat atatgttgca tcagttgacg
    38641 aaagattaaa tgaacatgga tatataattc ctggtcttgg tgatgctgga gatagattgt
    38701 ttggaactaa ataaaaataa ataactataa atatttatta gggactttct tatatagtgt
    38761 atataatttt atgtaagaag gttctttttt atatattttt tctcaaaata ggaattattg
    38821 aattgataaa tttctcgaat tgagaataaa aagtttttac caattaaaaa agattgctta
    38881 ttttacaagg ttttatagaa tagtagtcta ttttaatgtg gcatgttagt tgcttacgat
    38941 taagattata ggtattaagt gtattagtta aattgagaat tgaatattaa taatattatt
    39001 gggagtggta atacatgaag aattgtatag tagtaagagg ggctggagat ttagcaacag
    39061 cagttattca taaattacac aacagtggtt ttaaagtagt tgcgttagaa atagaagagc
    39121 cattagctat aagaaggcaa atttcttttt gtgaggctgt ttatgaaaaa aaagtaactg
    39181 ttgaaggtgt agaatgtata ttttgcaata ctgaaaaaga aatagaagaa gttttatctc
    39241 aacataaagt agccttgatt atagacccaa aaggagaaaa attatctaaa ttaaatccaa
    39301 tcgttttggt tgatggaata ctcgctaaga aaaacttagg tacaactaaa gatatggcac
    39361 cacttacaat agctttaggt cctggatttt gtgcaggaga agatgtagat gttgttatag
    39421 agacaatgag aggtcataat ctaggaagaa taataactaa tggtagagca gaaaaaaata
    39481 caggagtacc aggagcaata aaaggttttt ctaaagagag agttatgcat tcacagtatg
    39541 atggcataat ggaaaatgta tgtaagatag gtgatatagt tgaaaaggga gacctacttt
    39601 gctatattaa taattatgaa aaaaaacatg aagttagagc aacaatatct ggagtcctta
    39661 gaggtttatt aaaggatgga gcaaaggtaa acaaaaattt aaaaatacta gacatagacc
    39721 ctagaaaaga agaagttaag aatgctttta caatatcaga taaagctaga tgtatagctg
    39781 gaggggtttt agaagccata ctaaacaatg ggatttatcc ccaaaattaa tttatattag
    39841 ataaagctat taatatttta ttaagatgat ataataaatt taatagatta aagagagcat
    39901 atgacgggta tttaaaattt aaatggtaaa aatgctcttt ttttattgga cattcaatta
    39961 acatgtgtat ataatataaa aataagtatt tatttaggtt gaaatctaat aaatatataa
    40021 taccagtaca taagcaaaga ggagaaaagt aaattatgag accatcatgg gatgaatact
    40081 tcatggaaat tgcagaagta gtgaaaaagc gttcaacttg tattagaaga caagtaggag
    40141 ctgtaattgt aagagacaaa caaatattaa caactggata taatggttct ccgagaaatc
    40201 ttgaacactg tgaaaatatt ggatgtaaga ggcaggaatt aaatattccc tcaggagaaa
    40261 ggcatgaatt atgcagagca ttacatgctg aacaaaatgc gataatacaa gctgctcata
    40321 atggtattag tgtagatggg gcaactttgt atgttactac aagaccttgt gttttgtgtg
    40381 caaaaatgtg cataaatgca ggtatattga aaattgttta tgaaggtgat tatccagatg
    40441 atatgtctac agaattatta aaagaagcag gaatagaact cataaaattt tagtggataa
    40501 aatttatatg acaataaatg tcaaattatg acactgaaat acaatggtat caaatgatta
    40561 aaagaaagta tttgaaaaat catcagatta gtagtaagat atattttata attaaataaa
    40621 tttatgagat agattaatta catgttattt attttataat aatgagggat actatttatg
    40681 agtaaaagaa atacacatac agaaattgca aagttgttta gtttaataag ccaaatagga
    40741 ttgatgattg taatatcaat attaggatgt actttcttag gaaaattttt agatagtaaa
    40801 ttgaatacta ctccagttct gacaataata tttttattgt tgggtgttgg aggtgctttt
    40861 agtggtgttt ataaaactct aatagtatat acacgaagga agtgatatat tggatataaa
    40921 attacttaac caggtaaggt cagtaacaaa aggtatagta atatttgatg tagttttaat
    40981 tgtgctaatg ttgataacat caacattaag taaaccagta ttattaggac ttatatttgg
    41041 ctctataata gctatgttaa attttagatt attggcgata tcactggaaa aatcagtgac
    41101 atttcctgct gggaaggcac aagcttatgc atcagcacaa tatgctgtta gattatttat
    41161 aatggcagtt gttctaattg catcagctac agtaccacat ttaaatataa tcggaacagc
    41221 attgggatta ttgagcacaa agtttgtcat attatcaaaa aactttataa ataaattaaa
    41281 gaggaaggag gcataagtat gaatcaagcg cattattatc tcataggaaa ctttaagatt
    41341 agtgaaacaa tactagtaag ttggggaata atggcctttt tagcgatagc atcttatttt
    41401 ttgactagaa acttaaaaaa agttccaact agtaaagttc aaatattctt agaatttgca
    41461 gtaggtggtc ttgctaaaat ggttacagat gttatgggta aaaatacagt aaaaagaatg
    41521 ccatatatga taccatacat aggaagcttg tttttatttt tcgcatgttc aaatttaagc
    41581 ccattacttg gattaaggtc accaacaacc gacttagata caactgctgc atgggcaatg
    41641 attacatttt ttatgattta ttttgcagga gttaaattta aggggttatc atactttaaa
    41701 gaattggctc aaccacttgc cataatgtta ccaatcaata ttataggtga gttagctaga
    41761 ccaatatcat taagtttccg tccatttggg aatattttag gtggaacaat aataatgggt
    41821 ctgttttatc aacttttagg ttttatttct agtttaatac caggattatc aataccaatt
    41881 ggagaaatat taataccagt cccattacac ttgtactttg acttatttgc aggattgtta
    41941 caagcatata tatttataat gttaacaatg gtatttatat caagtgcagg agagttagat
    42001 taagaaatca acttatatta agataaaaat aaaataattt aaaaaattaa agttttaagg
    42061 aggaaataat tatggaaaca gcaatagtag cggcagcatc agcaatagga gcagggatag
    42121 cagttgcaac aggtataggt gcaggtatag gtcaaggtat agcagcagct aaagctgcag
    42181 aagcagtagg aaaccagcca gaagctaaag gtgatataac atcaacttta ttactaggag
    42241 ttgctatagc agagtcttca gcaatatatg gtttggttat atcaataata cttctattcg
    42301 taaatccatt ctttaaatac ttaggaatgt agatttagtc cttaaatatt ttgagatagg
    42361 tttataggta aatcatctca aacttataat catgagatga actttatcat ctcgtgatta
    42421 taagtagtta tgatttatgg aaaggaggat aattatggaa aaagcactag taggtataac
    42481 gtgggaattt gtgttccaaa ttgttaatac ttttataata tttttacttc tgagaaagct
    42541 tttattcaag ccagtattaa atataattga atcaagagaa aatgatatta aaagtgattt
    42601 ggtagaaggc gaaaaagcaa aaaatgaagg tttagctctg aaaaaagaat atgagtcaaa
    42661 aatcaatttt gccaaagatg aaggtcaaga aatcataaaa caagcaacta taagagcaga
    42721 gcagaaatca gatgatatag taaatactgc aaagaaagat gctcttgata taaaagaaaa
    42781 agcaaacaaa gatatagaac aagaaagaca aaaggttata aatgaaatca aaaatgatat
    42841 atctaacata gcactacttg cagcatctaa agttattgaa aaagatttag ataaatctaa
    42901 acatgaagaa ttgatagaaa actttataaa agaggtaggg gaagctaaat gataaatgta
    42961 atagcaaata gatatgccga agccctattt caattaggag aagaagaaaa ttcaactgat
    43021 gtcttattca aagaactaga aaaagtagta gatatgatga ctaaagttag taaagatttc
    43081 tacaaagttt taaaatcacc attagtttca aaatcagaaa agaaaaactt ggtagaaatt
    43141 atttttagta aagaagtaag ttctaacata aaaaactttt taaaagtttt agtagataaa
    43201 gatagaatat catatttaga agatatcgaa ttagcttata aagaattatt aaataagaaa
    43261 aacaatgtaa ttgatggagt agcaataagt gctattccaa tgagtgaaac agatattaaa
    43321 gaacttgaag tcaaactttc aaataaatac aacaaaaatg ttactataga aaatgttgtt
    43381 gacaagacta tactaggggg agttctagtt agaattggaa atgaacaaat agatggcact
    43441 gtgaaaactc gtcttgataa gatgaaagaa aagctatctg aagtaatatc ttaagaatgg
    43501 cggtgaaccc atgaacttaa aacctgaaga aataagttct ataattaaac agcaaataaa
    43561 aaattatgag aataaagttg agttgacaga tacaggtagt gttttaacag ttggggatgg
    43621 tatagcgagt gtatatggat tagaaaaagc tatgtctggt gagttgctag agttcccagg
    43681 tgaaatatat ggtatggcac ttaacttgga agaagaagta gttggagctg ttatccttgg
    43741 agatgacagt gaaataaaag aaggcgatat agttaagaga actggaagaa tagttgaagt
    43801 tccagttgga gaagctttaa taggtagagt tgtaaactca ctaggtcagc caatagatgg
    43861 aaaaggacct atagcgtaca caaaaactag accagttgaa tctgaggcac ctggtataat
    43921 agatagaagg tctgtttatg aaccattaca aacaggaatc aaatcaatag attcaatgat
    43981 accaattggt agaggtcaaa gagagttaat aataggtgac agacaaactg gtaaaacatc
    44041 tatagttatt gacactatac taaatcaaaa aggaaaagat gttatctgta tatatgtagc
    44101 aattggacaa aaacgttcta ctatagctca attagttagc tctttagaaa aaggtggagc
    44161 tttggattat acaatagtag tttcagcaac agcatctgaa tcagcaccac ttcaatatat
    44221 agcaccatat gctggagcag ctatgggtga agaatttatg tacaatggga aacatgtact
    44281 tatagtgtat gatgatttaa gtaaacaagc tgttgcatat agagaaatgt cattgttact
    44341 tagaagacca ccaggacgtg aggcatatcc tggagatgta ttctatttac attcaagatt
    44401 acttgaaaga gcagctaagt tatctgatga attgggtgga ggttcaatga ctgccttgcc
    44461 tataatagaa actcaagctg gtgacgtttc tgcatatata ccaacaaatg ttatatctat
    44521 aacagatggt caaatatact tacaaccaga gttattctat tcaggtgtaa gaccagcagt
    44581 tgaccctggt atatcagtat caagggttgg tggttctgcg caaattaaag caatgaaaaa
    44641 agttgcagga acattaaaac ttgcatattc acaatataga gaacttgcag cattctcaca
    44701 gtttggttct gacttagatg aagatactaa aaagagactt gctcaaggtg aaagaatcgt
    44761 tgagatatta aaacaaggtg agcatcaacc aataaaagtt gaaaatcaag ttatgataat
    44821 atatgctgtt ataaataatc atttagagga tattccgata gataatatag ctagatttga
    44881 atcagaattg tatgcatttg tagataataa ttatccggaa atatcaagaa aaatattagg
    44941 tggagaagac ttcactcatg atttaacaga tgcaataaat gaatttaaag aaaagtttgt
    45001 tgttgaagta taatcctttt taaaaaagga ggtaaattaa ttggcaggag ctggaatcaa
    45061 agcaatcaaa acacgtatgg gaactgttga aagtacaaag caaataacta aagcaatgga
    45121 attagttgca tcttctaagc ttagaaaagc aaaagaaaag gctgaagatt caagagaata
    45181 ttttaatact ttatacaata cagtaaaaga tattgcccaa acaacacgtg gaattagaaa
    45241 tgaattttta aaagaaagag aagtaaaaaa taaatgctat gtagtagtag ctggagacag
    45301 aggacttgca ggtggataca attctaatgt attaaaggaa tctgtctctc atatgaaagg
    45361 taaaaaggaa acagtgatta ctgttggaaa gaaagcacat gaattttttt ctaaaagaaa
    45421 ctacaatata gcaaaagata taaatagtgt tgaagcttgt ggatatgaag atgcgacaga
    45481 aatttcacaa actattattg acctgtataa aaaaggtgaa gtagatgaag tttatattgc
    45541 ttatacaaaa tttaaatctg ctttatcaca agaagttgaa attttgaaat tgcttccatt
    45601 agtctttgaa aaagatacag acgaacaagg aatggcagta aaagaagaaa aacaaaatag
    45661 atcaaaagta caatatatac catcagcaga agcagtttta agttatattg tacctcagtt
    45721 tatatcaggt acagtatatg gtggaatttt ggaatctttt gcatcagagc aaggtgctag
    45781 aagaactgca atggaatcag ccacagacaa tgcgaatgaa atgctatcta atttagagtt
    45841 aagttataac agagcaagac agtcagctgt aactcaagaa ataacagaga tagttggtgg
    45901 agttgaagct ctaaaatagg gaggttagga aatggcaaat gtaggtaaag tagtacagat
    45961 tgtaggagct gtactagatg ttaagtttga tagtgaacaa agcctaccta acttattaaa
    46021 tgcgttagta ataaagttag gagataaaga aatagttgca gaagttgctc aacatatagg
    46081 tgatgatact gtaagatgta tagctatgag tgcaacagac ggacttgtaa gaggtatgga
    46141 agttgtagat acaggagggc caataagtgt tcctgttgga gatgaaactt taggtagaat
    46201 attcaatgtt cttggaaaac cagttgatgg gaaaccagct ccaaaatctg cacctaaatt
    46261 acctatacat agaccagcac cggcgtatga tgaactagaa actacagcag agatactaga
    46321 aactggtatt aaggtagttg acttactagc tccatatcta aaaggtggaa aaataggtct
    46381 atttggagga gcaggagtag gaaagacagt tctaatacaa gagcttataa acaatattgc
    46441 taagcaacat ggtggtattt ctgtattttc aggagtagga gaaagaacaa gagaaggtaa
    46501 cgacctttat ggcgaaatga gtgagtctgg agttataaat aaaacagctc tagtatttgg
    46561 tcaaatgaat gaaccacctg gagcgagaat gagagttgct ttaactggac ttacaatggc
    46621 agaacatttt agagatgagc aaggacaaga cgttttactt ttcgttgata atatattccg
    46681 tttcacacaa gctggttcag aagtttcagc acttctagga cgtatgccat cagctgttgg
    46741 ttatcagcca acattagcta ctgaaatggg tgcacttcaa gagagaataa catcaactaa
    46801 gaaaggttca ataacatctg ttcaagcagt atatgtacca gcagatgact taactgaccc
    46861 agcaccagct acaacattct ctcacttaga tgcaaaaaca gttttatcaa gacaaatatc
    46921 atcactgggt atatatccag cagttgaccc tcttgaatct acatcaagga ttcttgaccc
    46981 tagtatagta ggaaaagaac attatgaagt agctagaggt gtacaatcaa tacttcaaag
    47041 atataaagaa cttcaagata taattgctat acttggtatg gatgaattat ctgatgaaga
    47101 taagttaata gttgcacgtg ctagaaagat acaaagattc ttatctcaat cattcacagt
    47161 tgctgagcaa tttacaggaa atccaggtca atatgttcct gttaaagaga ctgtaagagg
    47221 atttaaagaa attcttgaag ggaaacatga tgacttacca gagtcagcat tcttatttgt
    47281 aggaacaata gaagacgctg ttagaaaagc gaagggaagt atgtagtcat ggccagtgaa
    47341 ttttcattga aagttgtaac tccaagtaat actttttatg atggagatgt tgaaatgata
    47401 gtagttagaa ctacaaaagg agacaaaggg gtgttgaaag gtcatatacc atttgtttca
    47461 ggtatacctg taggaacttt aaagataaaa aaagagggaa gttttaaaga ggcgaaaata
    47521 gcagaaggat tcattaatgt aactcaagat aaaactgtca tattgacaga atctgctgag
    47581 tggatttaat aaattaattt agagtataaa aagtcatttt gaccaaaaaa acttttgggt
    47641 aaaatggctt ttttgtgttt tggtgttaat aaaataacga taaaattgta agtttttagt
    47701 gataataaat tcacaaaatg tttaaattta ctgccaaatt atgttgtaat taataagata
    47761 aagaagattt agatagcttt tatgtaagtg aaaaagtctt catataagaa atacatataa
    47821 aatttttata aaatttaaaa aatttgctta gttgtgtagt ataataaata atataataga
    47881 tttgt
//
LOCUS       DACULQ010000007        46947 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.007, whole genome shotgun sequence.
ACCESSION   DACULQ010000007 DACULQ010000000
VERSION     DACULQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.007"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1074)
                     /locus_tag="JA154_01630"
     CDS             complement(<1..1074)
                     /locus_tag="JA154_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAT7994559.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNV"
     gene            complement(1167..2039)
                     /locus_tag="JA154_01635"
     CDS             complement(1167..2039)
                     /locus_tag="JA154_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HAT7994560.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            complement(2141..2476)
                     /locus_tag="JA154_01640"
     CDS             complement(2141..2476)
                     /locus_tag="JA154_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HAT7994561.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            complement(2479..4002)
                     /locus_tag="JA154_01645"
     CDS             complement(2479..4002)
                     /locus_tag="JA154_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD"
                     /protein_id="HAT7994562.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            complement(4023..4382)
                     /locus_tag="JA154_01650"
     CDS             complement(4023..4382)
                     /locus_tag="JA154_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HAT7994563.1"
                     /translation="MMELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKA
                     HNELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPI
                     VKQIRDTWHEVYNQLKI"
     gene            complement(4379..4774)
                     /locus_tag="JA154_01655"
     CDS             complement(4379..4774)
                     /locus_tag="JA154_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HAT7994564.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            complement(4796..5008)
                     /locus_tag="JA154_01660"
     CDS             complement(4796..5008)
                     /locus_tag="JA154_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HAT7994565.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            complement(5002..5394)
                     /locus_tag="JA154_01665"
     CDS             complement(5002..5394)
                     /locus_tag="JA154_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HAT7994566.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDSGC"
     gene            complement(5410..6342)
                     /locus_tag="JA154_01670"
     CDS             complement(5410..6342)
                     /locus_tag="JA154_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HAT7994567.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            complement(6361..7671)
                     /locus_tag="JA154_01675"
     CDS             complement(6361..7671)
                     /locus_tag="JA154_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HAT7994568.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            complement(7689..8105)
                     /locus_tag="JA154_01680"
     CDS             complement(7689..8105)
                     /locus_tag="JA154_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HAT7994569.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            complement(8110..8385)
                     /locus_tag="JA154_01685"
     CDS             complement(8110..8385)
                     /locus_tag="JA154_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HAT7994570.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            complement(8537..8851)
                     /locus_tag="JA154_01690"
     CDS             complement(8537..8851)
                     /locus_tag="JA154_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAT7994571.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            complement(8875..9303)
                     /locus_tag="JA154_01695"
     CDS             complement(8875..9303)
                     /locus_tag="JA154_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 domain-containing protein"
                     /protein_id="HAT7994572.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(9312..9980)
                     /locus_tag="JA154_01700"
     CDS             complement(9312..9980)
                     /locus_tag="JA154_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAT7994573.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            complement(10010..10993)
                     /locus_tag="JA154_01705"
     CDS             complement(10010..10993)
                     /locus_tag="JA154_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAT7994574.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            complement(11031..11588)
                     /locus_tag="JA154_01710"
     CDS             complement(11031..11588)
                     /locus_tag="JA154_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAT7994575.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            complement(11637..12506)
                     /locus_tag="JA154_01715"
     CDS             complement(11637..12506)
                     /locus_tag="JA154_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HAT7994576.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            complement(12615..13487)
                     /locus_tag="JA154_01720"
     CDS             complement(12615..13487)
                     /locus_tag="JA154_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAT7994577.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            complement(13693..17499)
                     /locus_tag="JA154_01725"
     CDS             complement(13693..17499)
                     /locus_tag="JA154_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086693.1"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAT7994578.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDNKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVIGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            complement(17524..18774)
                     /locus_tag="JA154_01730"
     CDS             complement(17524..18774)
                     /locus_tag="JA154_01730"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086692.1"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAT7994579.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTRVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            complement(18785..20317)
                     /locus_tag="JA154_01735"
     CDS             complement(18785..20317)
                     /locus_tag="JA154_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAT7994580.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAVVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(20310..20903)
                     /locus_tag="JA154_01740"
     CDS             complement(20310..20903)
                     /locus_tag="JA154_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAT7994581.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(20897..21973)
                     /locus_tag="JA154_01745"
     CDS             complement(20897..21973)
                     /locus_tag="JA154_01745"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086689.1"
                     /note="catalyzes the formation of
                     1-(5-phosphoribosyl)-5-aminoimidazole from
                     2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in
                     purine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAT7994582.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(21967..23334)
                     /locus_tag="JA154_01750"
     CDS             complement(21967..23334)
                     /locus_tag="JA154_01750"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086688.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAT7994583.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(23346..24044)
                     /locus_tag="JA154_01755"
     CDS             complement(23346..24044)
                     /locus_tag="JA154_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAT7994584.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(24050..24526)
                     /locus_tag="JA154_01760"
     CDS             complement(24050..24526)
                     /locus_tag="JA154_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAT7994585.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            24976..25488
                     /locus_tag="JA154_01765"
     CDS             24976..25488
                     /locus_tag="JA154_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAT7994586.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            complement(25872..27035)
                     /locus_tag="JA154_01770"
     CDS             complement(25872..27035)
                     /locus_tag="JA154_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT7994587.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(27032..27430)
                     /locus_tag="JA154_01775"
     CDS             complement(27032..27430)
                     /locus_tag="JA154_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAT7994588.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            27879..28094
                     /locus_tag="JA154_01780"
     CDS             27879..28094
                     /locus_tag="JA154_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994589.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAMQQFIVENEILISEEV"
     gene            28100..28393
                     /locus_tag="JA154_01785"
     CDS             28100..28393
                     /locus_tag="JA154_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HAT7994590.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(28588..29304)
                     /locus_tag="JA154_01790"
     CDS             complement(28588..29304)
                     /locus_tag="JA154_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT7994591.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(29392..30657)
                     /locus_tag="JA154_01795"
     CDS             complement(29392..30657)
                     /locus_tag="JA154_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphate kinase"
                     /protein_id="HAT7994592.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            complement(30761..31069)
                     /locus_tag="JA154_01800"
     CDS             complement(30761..31069)
                     /locus_tag="JA154_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAT7994593.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            complement(31136..32191)
                     /locus_tag="JA154_01805"
     CDS             complement(31136..32191)
                     /locus_tag="JA154_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAT7994594.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            complement(32193..32645)
                     /locus_tag="JA154_01810"
     CDS             complement(32193..32645)
                     /locus_tag="JA154_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT7994595.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            complement(32642..34672)
                     /locus_tag="JA154_01815"
     CDS             complement(32642..34672)
                     /locus_tag="JA154_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HAT7994596.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKDWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            complement(34928..37162)
                     /locus_tag="JA154_01820"
     CDS             complement(34928..37162)
                     /locus_tag="JA154_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT7994597.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            37481..40909
                     /locus_tag="JA154_01825"
     CDS             37481..40909
                     /locus_tag="JA154_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HAT7994598.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHQDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLKGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            41094..41927
                     /locus_tag="JA154_01830"
     CDS             41094..41927
                     /locus_tag="JA154_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7994599.1"
                     /translation="MSWKHIKIPNGDTMIFCNSFITRKSSMNIVVTHTPIVTTLEMQK
                     AYEPLAKHNVNIFAFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYG
                     NTGIGGMFAQYYACSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHI
                     TVKPPKYKGYNEVYDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAKDSKIKDGVNIP
                     TLVFKTLHDRYFKTQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVEN
                     Q"
     gene            complement(42056..42577)
                     /locus_tag="JA154_01835"
     CDS             complement(42056..42577)
                     /locus_tag="JA154_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HAT7994600.1"
                     /translation="MFSIGGGYAIILLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            42572..42922
                     /locus_tag="JA154_01840"
                     /pseudo
     CDS             42572..42922
                     /locus_tag="JA154_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(43070..44461)
                     /locus_tag="JA154_01845"
     CDS             complement(43070..44461)
                     /locus_tag="JA154_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAT7994601.1"
                     /translation="MRNKIIIGITFSLVLLMSGCASKQTAEINVLATTDLHGVIPYEL
                     TSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGDNNTESYIETPISK
                     DMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTYKKNGDSYTKPYII
                     KNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVE
                     DAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQ
                     IEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDKSSKLIKFEKNQES
                     DKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDIYKTIGYKWRNISS
                     PKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVTIYPNKNDKFKIEK
                     GKQEYSINLKHIQ"
     gene            complement(44906..45030)
                     /locus_tag="JA154_01850"
                     /pseudo
     CDS             complement(44906..45030)
                     /locus_tag="JA154_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(45179..45562)
                     /locus_tag="JA154_01855"
     CDS             complement(45179..45562)
                     /locus_tag="JA154_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7994602.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDGNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEENLSDDKLDMLEEWVKNWEEDE"
     gene            complement(45656..46045)
                     /locus_tag="JA154_01860"
     CDS             complement(45656..46045)
                     /locus_tag="JA154_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7994603.1"
                     /translation="MKQQEVVNKLRRGELMLMEYLWRKNSILSKKEIIEAMKEKYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNAIKEDRKVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKE"
     regulatory      46254..46351
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(46553..46696)
                     /locus_tag="JA154_01865"
     CDS             complement(46553..46696)
                     /locus_tag="JA154_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994604.1"
                     /translation="MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNK
                     KRH"
BASE COUNT        15012 a         8239 c         5186 g        18510 t
ORIGIN      
        1 cacattcttt ttcttttcca catcttcaat gttattatac tctgaaatta caaactcata
       61 tttttcaagc ttataataaa tctttatcag tgtaataata gcctcatctc taagccctac
      121 agtatcagtg tctgaaaata aactatttgc ttgtgtactt atttcataat tatctaacaa
      181 gtaaatatat ctcttatacg aatctatact attttcatac tttcctaaat ctacttgtgc
      241 ttgtgcaata taataatata tatcaatatt attcatgtct ttcttaatat actttaagca
      301 caatctttca cacttcttat gttttcctaa atataaatat gtttttacaa ggcttgttat
      361 caagtaacca ggtgccaatt ctagtttaga atataattca taagatttct caagatagta
      421 taaagcctcc tgatattttc ctaatataat aaaatttttc cctaattgat agtttgtata
      481 tggagaatta tcattctctt ttaactcttg aaataatagt ttttcatttc ttttcacctt
      541 atttaatctt atttcttcat cttcaaacaa atatccataa tgttcaaaat cagcaacatt
      601 attaaatata gggtttttgt atattggttg ttcatgtact ttaccatggt atctaaaatc
      661 aacgttttta aaaagtctaa gcactgaaca atatccatat aattttttat catatgagta
      721 aagatttttt aattgtattg aagcagaatt aaatttttta tcaagaccat ctttaaaaaa
      781 ttttacaata gttgaagaat caattagtat ttcatctgca tctaatataa gtaaccattc
      841 acctttagca taggatattg atatatttct catactagaa aaatcattat tccaattatg
      901 aaaatacact ttttccgtgt acttcttaga tatttttata gtatcatctg tagaacctgt
      961 atcgactatt ataagttcac tttctataga atttcttaat gtggttaatg attttaaagt
     1021 tttttcaagt attttttctt catttttaac aatcataact atactaagta acatataaat
     1081 tttcctttca aaagtatatt tgtaaaaaaa gaaaggagta aagcaaagcc ttaccctttc
     1141 ttctatatcc aatatacaaa attattttat cctaataatt gtaaaactcc ttgtggttgt
     1201 tgattagctt gagcaagcat tgactgtgat gcttgaacta atatattcat tttagataag
     1261 ttaaccattt ctgaagcaac atctgtatct cttattcttg attcagctgc tgtaacattt
     1321 tctatagtat tatttaagtt gttttgagtt gattctagtc tattttgttg tgcccctaat
     1381 tttgctcttg atgtatttaa ttctttaaga gctacatcta agctagatac catttgttta
     1441 gctgcatttg ttccagaagt tccatctgca tttaagcttg ctattgtaga acttgttata
     1501 cctgctttac tcattatact tgaagtattt actagagcaa cttttatttc attgttgttg
     1561 ttagatgttc ctgcaacatt tgttccaaag tttgctccaa cttgtaatct tatttctgta
     1621 gaacttccat caagtaattt cttaccatta aactcaattg agcttgatat tctttcaact
     1681 tcatctttta attgtagtaa ctcatcagct atcttttgtc tttcttcagc agtattagtt
     1741 tcatttgaag attgtactga taaagttctc attctttgta gtatgttacc agtttcttct
     1801 aatgcacctt ctgctgtttg tacaacagat ataccatctt gaacgtttct tcctgcttgg
     1861 tctaaacctt ttatttgagc tctcattttc tcagatatag caagcccagc agcatcgtca
     1921 gcagctctct taattctaac accagaagat agcttctcca tagacttact ttgtgcattt
     1981 acgtttcttc ccatttggtt atttgctatc aaagcactta catttgtatt aactctcatt
     2041 attttaccct ccttagtata gttgacatcc ttgtcatttc tacttttaat cttttttatt
     2101 aattcttcat ttatataatc ggacaagatg gtacataact ttatactttt ttattaaaaa
     2161 tatttacttt ttctctattt aagttataat acattgtgtt cacttgttta gtaagcttat
     2221 actcatgtaa ttcttttttt acttctaata attgtccttg taaaatatct cttatcctgc
     2281 tatcaatttc aattaattcc attctattat aaagctgtat aaaatcattt ctatcaattt
     2341 cacttacact atttataatc tcttgtcttt cacctaactt aatacttatc tttatatatt
     2401 cttccttttc aatcaaattt ataatttgta aagatatatc tttatataaa tttaattttt
     2461 cttttaattt atccataatt aattaccctg tgcttgtgag aaataattca tttgggaaga
     2521 atactgattc aacaaagatt ctaaggctga atatttagca taaagtcttt tctctttatc
     2581 attcattttt tcaactaacc tgcttatatt tctttcttgc tctgcaattt gttttgaata
     2641 tacattttca ctagcagaag cagttttttc tatacctgct tttttaggaa gtttagaagt
     2701 tgaacttcca gaatattcat atacaatatc ttttagcttt tcaaacattc ccttatcatc
     2761 ataattcgaa gaataacctg ctagtgtttt gtatacctta tcactatcat ctataagagc
     2821 ctttgaaaaa gtatctgcat taaatgatat ttgacctttt ttattgtaat tttcactttc
     2881 attgatacct agttttctta atgcaataat attttttcca tctccaaaaa atgcagattg
     2941 aatatcttca acaaaacctc ttaactcatc atcatttcta agtatacctt ctttagcttt
     3001 cttttcccat ttttctattt cttcagttgt catatcttct ttttgggcat ctgtaagagg
     3061 cggataatct tttggttttt tagtagtaac taaaccatag accttgtcca tcagtttatt
     3121 ataatcatcc acaaatgctt gcatattttt aacagcttct tcagtatcag tgacagaagt
     3181 caactttgca gttccttttg aatttacatt atagtcaata ttatctatat taaatgaatt
     3241 ttgttcttta cttatatgag ttactcttcc atgttcatca gttacatcag cttctagatt
     3301 ctttccatat acatttgcca ctttgtttcc accagcatca tttataaatg tgataccatt
     3361 atcattagca atttgctgag ctaaattgtc atttccaatt actcttaaat taatgtctga
     3421 tgacgaacca gtctgttttc ttgaaataat caattcacct gtcatctcac tataagaagc
     3481 ctttatatca ttatctgcgc aaagggagtt tatcttactt acaatagtac ttgtagtatc
     3541 ttgttcagat attggtatat ttacatctcc tatagttaaa gaactcgctc cactagcatt
     3601 tggaggaaac tgttgaacaa taggttcact tgaatttaat ttaatagtaa attttgctgg
     3661 ttcagccatt tgagaaactg caaattgata atttattttt tctgcaccag ctgagccttt
     3721 tgctactata atactttcat cagaactggt tattcttgta gaagagtatg ctttttcact
     3781 tactatacta ttaggagaat ttacgcttag atatttatca taaagatctt ttgattcttg
     3841 tataacattt ctatatattt cttgcttcca tttaacgatt tgttgttctt gttttgcttt
     3901 atcaactttt tccttgtctc taatcatact agcttcgatt atgccttcca tatcaaaatt
     3961 tcctgaaaga cctgtaactc ttataggact tatacttgac atacctttat ccctccatcc
     4021 aattatattt ttagttgatt gtaaacttca tgccatgtat ctctaatttg ttttactatt
     4081 ggcaatatat catcaattat tttaatatct ttattttcat cagctattgc taattcagat
     4141 tttataaatt cataaacttt atacatatcc tttgcccatt gacctgcatc ttggtctaat
     4201 gtagtcatta gttctgtaaa tatattttga actcttacaa gttcattatg tgctttcaaa
     4261 tagtcgttat ttaaaagagc ttctttggat atattggtat attttactgt tccatctaca
     4321 agcatcaata gtaattcctc ttttgataac ttatctaatt cagctaaatt taattccatt
     4381 atttgtacct acttctttct tctttacttt ttttatcagc ttcatgccaa gtatctttaa
     4441 tttgttctat tacaggcaac acattatcta ttatttgaat gtcttttcta atattagctc
     4501 ttgaaagttc atatcttaca aaatcataaa ctctgtacat atcctttgcc cattgacctg
     4561 cattttggtc taatgtaacc attagttctg taaatatatc ctgaactctt ataagctctc
     4621 tatgcgcttt ttgtgtattt ttatctaaga tagctgtttt ggctattttg gtatatttta
     4681 ctgctccatc aacaagcata agtaacaatt gttcctttga tgccataaaa acagcatttt
     4741 gtttataaga attataagga ttttctccat acatatatta cttcctccaa gcttattatt
     4801 ttaatgactt taaaattttt atatttttat ttgtagcttt tatattctca ctctttactt
     4861 tttcgtatat ttcttttctt aatatactta tattgttagg cgctgatata gctagtttca
     4921 cattgtttcc atctacccct acaattttta cttctatgct ttctcctata agtactgctt
     4981 catctttttt tcttgaaatt actagcatcc actatcacct ctcaataatg gatgccttat
     5041 tgaatattta tcatcctgta aaattatttg cattccaaaa ttagttttta tatttatcac
     5101 tataggagct ttcatgttca cagttgattc ttcaaatgtc tgaccaagag taattatatt
     5161 aaataataca acatcctttg gagatttcac ttctagtttt tctattgttt cctgatttaa
     5221 aactatttca tattcattga taatctcaaa tggagataca acaacaaatc ctataccttc
     5281 ttcttcagta gaattaaatt cttttaaata atcattatct tctatatcta aaagctcata
     5341 ttcttttaaa ttttcaaaac ctaatatacc tttttttaat gtaaccttca tcatatctgc
     5401 gttattttat tatatgtaat ctaatataga tacattaaat aactttcctc ctgtttgtag
     5461 tgaagcttga taagttaact ctgctgcctt taattgaaca agtttttcac cataatctat
     5521 ttcttcattt tgtgacaaaa tatcctttaa ctgtatcaaa ctatcatcgt ttttttcctt
     5581 catagcctct actgtatttg cttttgtccc atatatagac ctaacattta ctgtattttc
     5641 tattagttgt tccatatcgc ccaaacaagt tgttgtaagc tcttttatag catcagcatc
     5701 tggatttgct aatttttttg atatgttatt taactcttca aatgggtcag tttttgttgg
     5761 gtcaaatcca aggctactca atttaacgtt ataatctaca cttattcctt gagctatatc
     5821 catactcaaa gagtcattta atttattaag atttagacta gaattaaact ttaaactcac
     5881 tgaaccatca gcattatcta tacattcaac aggtggtacc cccgtatttg aacctgaaaa
     5941 tatgtacttc ccttcatgtg ttgcatttaa agcatttgca atttctttca ttttttcatt
     6001 agtttcatta ctaagagtct ttaattcatc atctgagtat gcaccatcac cacttgccac
     6061 caaagttttc tttatgtctg ataataattt tccaagctga tctaatgcag tatcagtagt
     6121 ttctaaccaa ttccctgtat ctttacaatt ctcaacataa gtctccatag tctttatttc
     6181 actttttgag tttaatatct ttattgcttt atatggatta tctgatagct tgtttatttc
     6241 ttttgttgta ttaacttgtc tattaacctt atccaatctt tgtagattat cttgtagtga
     6301 gtttagatag ctgctagaca tcatacctgt actgactctc attataaatt cccccttcta
     6361 ttaaattatt ccatttatta caacatccag tagttgcaca gctgttgaca taacttttgc
     6421 actagcttga tatgaacgtt gtatttttat aagctctgtc atttcttcat ccaaagatac
     6481 acctgaaaca ctcattcttg agttatcaat attatttata attttacgtt gatttgcttc
     6541 atcttgattt actttttgag aatctaaacc gattgattct gcaaaggctt tatagtggtc
     6601 acttattgta cttacttttc caccagccac cccaatatct attttttcat cctttaactt
     6661 agcaattttt gaagcttcat cagcagtaag ctttaattca tttgggttac tctttatagc
     6721 tggatttatc tttataatat cttcaccatc tacatttgaa gttataaaaa aatctgttac
     6781 tgttattcct gcagacgtat tataaacatc atttatctgt ttagctaatc catcagatac
     6841 atcttttaaa tcgcttttat atgtatcaat cttaccatgc atttcaaaca agccttgtaa
     6901 ggtccctgat atacctgttt tccaagttcc atctggattt tttaattcat taagttctaa
     6961 tttaccatct gcaagcatat ttttaacatc actattttca aagtctagtt taaaactcaa
     7021 gttatctaaa attctatctc tttcatcaag taaatcattt ggtgtagagc cagaaccaga
     7081 tataatatca atagatttat ttaaatcttc aagacttttt aacatgtcat ttatttgttt
     7141 tatctcagtg tctatattag aatctatatc ttcttttaac ttatcaagct tcttataact
     7201 ttcacttatt aaagttgcta aattagatgc gttttctacc actagacttt ttttactgtc
     7261 actatttata tcctttgata atgactccca tcctttaaaa aaatcatcta tacctgcaga
     7321 tattccatag tctgatggtt catttagtat agtttctata gtattgtaat aatcatattt
     7381 tgaaactgtc ttaccatact tatgagcttc tgccctaaat tggtaatcat aaaaactatt
     7441 tctagctctt gttacatccg ttacatgtac tccagtccct aattgtcctc ttgcaattga
     7501 attactcaaa ctaggcatat acattggact atttgtggca aactcggtcc tttgcctaga
     7561 ataaccgtct gtatctagat tacttatatt atgagaaatt gtctgtattg ctttttggtg
     7621 cgcattcatt cctgaattag ctgtatttaa aatggaaaat aatcctgcca tatttctcct
     7681 cctaatattt attttctaat atttccataa gaattataag ttgtagtttg cttatcagct
     7741 ggttttatga aattaatcat ttttttcgta aaaataagtt ctttctttat tagagaatca
     7801 tttgattctt gttgattttt agctaaagca agaatgtgtt tcaagtctgt atatatttct
     7861 tttaagtatc catcattact attttcaaca acttcactca ttgaaatatc atcaccaaaa
     7921 agttgtcttc tctttatttc tatggatgca agctccctgc taatatcttc tagtttagga
     7981 tttaacatat ttaaattttc taagtctttt tcaactatat aatcaaattg ttcttttaat
     8041 aattccatca tcttactaat tatatttttt tcttcatata ttataacttt taagtctgta
     8101 atcatgactt tatcctcgca tatcctctat catttttttc gcaatttctt ttggttttat
     8161 tttgtaagtt ccattttcta atgcattttt tatttcatcg acttttttat ttctaatatt
     8221 ctcatcactc atatttgaca cacttgccat ttcctttgca aatttagata actctatctt
     8281 atctgtttta ttaatttttt taacctcttc cacttttttt ataccagatg tattctttaa
     8341 ataattattc actacaatat ttgaacttac atttttaata tccatagttt cacctccaaa
     8401 attacatact tataatttag ttatcgctca taaaataaaa atatttatat aattcacaca
     8461 tattttaaat tttttataaa aatatcccct tagctaaact catacagaat agcctaaagg
     8521 gatactattt aaaatgctaa tttacatctg gatcttctat tttagataat cttacagata
     8581 acctctcatt tgtaataatg ctttctccat aagcaaacaa tctatcgttt gcatatatat
     8641 ctaatttttc atccacattc ttacttaatt ttattataga gccttctttt aaatctaata
     8701 tattttttat tgtttctgtt gtatttccta tagaaacagc tacatttact tttgcattaa
     8761 aaacattttg attttcttct ataacaccag atttttcttc tattaattgc ccaaaattca
     8821 attcatatat atcctctttt ttgctattca taaaattttt ctccttgtat aaccttatct
     8881 atacatatct atcaataagc cttgtatcat gtcaacagta gaaacaatat ctatgttttc
     8941 tttttcttca ctaattaact tactagtcat atcctcaagt ttactatcaa caatctttac
     9001 attcttataa tactgatgct cccaaaagct actattttta tcaagtgaat acatattatc
     9061 tacaacagat tttaggtatt cttttactgc atttttatat atactaatat cgccatagtt
     9121 ttgagtaagt acaagccttt ctcctgcctt ttttacactt tccaaatttc gttttatatc
     9181 ttccaatgaa gaagattgtt ttaaagagtt taatttgtca gaaaatttat tactctcttt
     9241 tagtttttta acctccatat ttatacttgg acttatgttt agcttatcta tatttattcc
     9301 cataataaag cctaccctct ataatatttc ataaccttag gaatataatt ttgtgtttct
     9361 tttggcattt tatacaggtc agagattgat tctactcctc tacttttcat tcttccagga
     9421 ccaccattat atgccatcaa tcccatttct atgctccctc caaacatatc aatgtattcc
     9481 ttaatatgtc tagttccagc atctatattc tgttctatat taaaagggtc tgtaactcct
     9541 aagtctctgc agttttctgg cataagttgc ataagaccct tagcaccagc agaagaaacg
     9601 gtatttggat taaaatctga ttcaacttta attattgctt ttataagatt tgaatctacg
     9661 ttatattttt ttgatgcttg atctacagca ttttcaattc tcaactttac atttttatct
     9721 tttgtatcaa catctataaa tctactagtg gtattattta ctacattatc aagttcatta
     9781 aataaattct cagaattaga caataaattg tagctattag aagtatgttg attattttgt
     9841 gataatgctt ttaacaaggt catcataatc atatcaaatc cagatgaatt attatatgta
     9901 caattacatt gatttgtagt attactacca gataattgag tcaatgctaa tatattaatt
     9961 agtgaactta tctcattcaa tataaaacgc ccctttctaa attttaaagt tatactgata
    10021 aaacagcttt ccaccaagat tcattttcta tataccactt aacagtttct tttataccca
    10081 aatcaaagtc atattttgcc ttccaaccta actcattctc tattttagtt gcatcaatag
    10141 catatcttaa atcatgacca ggtctatcat ttacatagga tattaactct tcagatttac
    10201 ctaaaagatt taatatcatt tttactactt ctagattact tctttcatta tgacctccta
    10261 tattgtaaat ctcccctata tttcctttat gtattattaa gtctatagca gtacaatggt
    10321 catatacatg taaccaatct cttacatttt ccccattccc atatactggc aattgtttgt
    10381 tttccaaagc atttaaaatc ataagaggaa tcaatttttc aggaaaatga tatggcccat
    10441 aattgttgga acacctagat atagttgtta acaatccata tgttctataa taagaactta
    10501 ccaataaatc tgctgatgct ttagaagctg aatatggaga acttggattg atagaagatt
    10561 gttctgtaaa gaataaatcc tttctatcaa ttggtaaatc accatacact tcgtctgtag
    10621 atatttgatg atacctttta actccatatt ttatagatgc atctaaaagt acttgtgttc
    10681 ctataatgtt tgttttaata aataaatcag gattctcaac tgacctatct acatgagatt
    10741 cagcggcaaa gttaacaact atatcaaacc tttcttcttc aaatagctta tacacctgct
    10801 ccctatttga aatatctcct ttaacaaatt tataattgga attgtcttgt atttctatta
    10861 atgtctctaa attaccagca taagtcaaca agtctaggtt aacaataaaa taatctacat
    10921 acttgttaag catataatgt ataaaattac tcccaataaa tcctgctcct ccagttatca
    10981 gtattttttt catatttttt ctcctaaatg ttaattctaa taaattttta ttaacgtctt
    11041 gtacaaatat ctttaaaact tttttgtttt ttgtcctttt ctgataaaaa tatttcttca
    11101 attcctttta aaggccactc aatagatatt tgcttgtcat tccatataaa gccattctca
    11161 aattctgggt aataataatt tgtacatttg taactaaata ctgctttttc tgataaaact
    11221 aaaaatccat gtgcaaaccc ttctggaata taaaattgtt ttttattctt atcagacaat
    11281 attacacctt cccatttccc aaatgtttgt gaatctttcc ttaaatcaac tgcaacatcg
    11341 aaaacttctc cctctgtaac tctgactaat tttccttgag gatatttaac ctgaaaatgc
    11401 attcctctta aaacgccttt tcttgagcta gactcattat cttgaataaa acacatatca
    11461 agaccctgct ctataaaatc ctttttatta tagctttcca taaaatatcc tctttcatca
    11521 taaaatacat ttggttcgac tatatataat ccttctattt tagtctttat aacattgatt
    11581 tttcccatta taataaactc ccttaataaa atttataata aattttatct accatattac
    11641 atatcttcac tagccaattt taatagatat tgaccatatt cagtttttat cagtggcttg
    11701 gctagtttca ttaattgttc tctacttata tatccttttg tatatgcaat ctcttctaaa
    11761 catgctatat acagtccctg tcttgtttga accacttcaa cataattaga agcatccaat
    11821 agacctttat gtgtaccagt atcaagccaa gccatacctc ttcctagaag ttccactttt
    11881 agttttcctc gttttagata ttcactgttt atagaggtta tttctaattc acctctatct
    11941 gacggttcaa ctagctttgc tatctctaca acatcattat cataaaaata taatcctggt
    12001 atagcataat tagatttagg aactgtaggt ttttcttcta ttgacaatac attaaaatcc
    12061 ttatcaaatt ctacaactcc aaacgaattt ggattactta catgataccc aaatatagtt
    12121 gctccttttc ttttaactgc attctccaac ctttcactaa atccatatcc ataaaatata
    12181 ttatccccta atacaagaga cactctgtca tttcctataa attcttctcc tattataaac
    12241 gcttctgcaa ttccatttgc tttctcctga attgcatatg ttatgttaag ccctagtgaa
    12301 cttccatctc caaataattc ttcaaatgct tttatatctc ttggtgtaga tattattaat
    12361 atatcttgta tccctgccaa cataagaacc gacatagggt aatatatcat tggtttatcg
    12421 tatattggaa gtgtttgttt tgatatacat tttgttatag gatatagtct tgtaccagac
    12481 cctcctgcta aaataattcc tttcatcttt ttccatcctt aaatttgttt ttgtcatatt
    12541 atgattatcg gataaataaa agaaatattt atatttttta taatatctta ttttcttttc
    12601 tcaaaataat acatttactt ttcatttata tcatatcttt ctaaatattc ttcaatggat
    12661 tcttcccact ttctaaaaga atttaaacca attaacttca acataaaatt atccaataca
    12721 gaatatagtg gtcttggagc cttacattta aattcgttac tcttaattgg atttactttt
    12781 atatctatat tttttagttt aaatatcttt ttggcaaaat cataccaact acactcacct
    12841 tcacatgtag catgataaac tccataatgt tctgtttcca tgattcttat tataattttt
    12901 gctaagtcta ctgtacttgt tggagttcca aactggtcat taaccacatt aacctcttta
    12961 ttttctaaag aaagttttat catggtcttt acaaaattat tcccattacc atataaccat
    13021 gctgttctca atataaagta cttatgtgag aattgttcaa caaatttttc tcccataagt
    13081 ttactttttc catatacaga attgggttca gttttattgt cttccctata tggatattta
    13141 gaaaaaccat tgaatacata gtcagttgat atgtgaataa gcttagcatt aactttttct
    13201 gatgctattg ctagatttct cactcctaaa gcatttattt tgtatgcaat ttcgatatta
    13261 tcttcacatg catcaacctt tgtatatgct gcacagttta caactacatc aggtttatta
    13321 tgcaaaataa aattatctac attagtctga ttggatatgt ccaaatcact tcttgttgta
    13381 gctaatataa catattttgg ttggtttata ctttgattga ttgcttctaa ttggttaacc
    13441 aattcttttc ctaattgacc attagaccct gttattaata ttttcattaa ttatgttgcc
    13501 ctccaataaa tattcaattt tacttaataa ttaattataa cacagcttgt attttaactt
    13561 aggttgaact tattatttct tagataaata aaaaagagat gtaatattaa atgattatca
    13621 tcatattatt acatctcttt aaatctaaat atctaaatta tatattctag ctatcaatta
    13681 aacatcaaag ctttatctaa aatatttaac tccagattca aatatctttt ggtctttttc
    13741 acctataata ttcttatata cttcctcacc aattctttca gaatgtccca ttttaccaaa
    13801 cactctacca tcagcactag ttataccttc aacagcatag aatgaaccat ttggattaaa
    13861 ctcaatatca taagtagctt cattattaaa gtcaacatat tgagttgcta cttgtccatt
    13921 agcaattaat tctctcataa catcttcact ggcaacaaac ttaccttctc catgagaaat
    13981 tgctatatta tgaatatctc caacattagt ttgagctaac caaggagatt tattagatga
    14041 aattctagtt ctagcaatct tagcttgatg gcgaccaata ttattgtaag taagtgttgg
    14101 agcactttca cttggaactc ttatttctcc atatggaact aaccctaatt taattaatac
    14161 ttggaatcca ttacagatac ctaacattag tccatctttt tgagttaaaa attcatttat
    14221 agcttcttgc actctaggat ttctaaatac agtagctata aatttaccag aaccatctgg
    14281 ctcatcacca gcactaaatc cacctggtaa cattattatt tgagaagatt ttatttcgtt
    14341 tactattgta tctatagaat cttctatgtc tttataagtt aaattcttaa atactcttat
    14401 acttgcatta gctcctgctc tttcaaatgc tcttgcagag tcatattcac agtttgttcc
    14461 agggaatgct ggtataaata ctcttggagt agctatactt aatgatgatt ttttagcttc
    14521 tccttgtgat atataactta tagtttctat ctttgatttt acttcttctg ttttagttgg
    14581 gaatataggc tctaaagtac tacaatgtgc tttatataat tcatctaatg caagttcttc
    14641 acctttgata aatatactag cttctttaac tgtacttcca atcacaacat aattgtatcc
    14701 ttctaacata cttaaatcat catttgctaa ttcaagtact atatttccat aactagcctc
    14761 aaataattta ttatctgtaa atgctttttc agcttcttca ctaaacttaa atcctatttt
    14821 attaccaaaa gccattttac ttattgcttc acctacacct gcaaatccta atgcatatgt
    14881 tgataaaact ttatttccat gtataagttc tcttacttta tctaagtttc tctttaattc
    14941 ctcaaaatct acaacatcat tttctgcctt gtttacacat aacattacta cttttgagtc
    15001 tgctttttta aattctggag atactacttt cttcgcatct actgtgtcaa cagcaaatga
    15061 aactaatgtt ggtggaactt caatatcttt aaatgtacca gacatactgt cttttccacc
    15121 tatagcaggt atctcaaatt tactttgtgc ataaaacgct cctaaaagtg ctgagaatgg
    15181 tttaccccat ttttctggat tgttaccaag tttttcaaaa tactcttgga atgtaagtct
    15241 tgttgtacta taatttcctc caatagcaac caatttacac acagattcta ctactgcata
    15301 caaggcacca tggaatgggc tccattttcc tacctttgga ttgtatccat aagtcattat
    15361 tgtagaagta ttagtttctc ctccaagtac tggtatctta gcaaccatac cttgtgttgg
    15421 agtcgcttga tactttcctc caaatggcat aagtactgta ttaccaccaa ttgtattatc
    15481 aaacatctct actaatcctt tttgagaaca aacattaagg tcagatagta cttttgtaaa
    15541 cttgtctttc atacttgcac acttaacatc ttttactatt tcattagagc taaagaatgt
    15601 attttcttca tctactttag taacatgaac atcagtagtt tgttttactc cgtttgtatc
    15661 taaaaactct ctatttatat ctacaattgc ttttccattc cagaacattc ttaaatatcc
    15721 tgtatctgtt actgtagcta catgcgtagc ttctaagttt tcttctactg ctaattgtat
    15781 aaatttatct ttattttctt tttttatggc tactgccata cgctcttgtg actctgaaat
    15841 tgcaagctca gttccatcaa gtccatcata ttttttagga actaagtcta agtttatatc
    15901 taagctatca gcaatctctc ctatagcaac acaaactcca cctgctccaa aatcattaca
    15961 tctttttatc atttgagcaa cttctttatt tctaaagaat ctttgtattt ttctttcgtt
    16021 aggagcatct cctttttgaa cttctgcact acaagttaag attgactctt cactatgttc
    16081 tttagatgaa ccagttgctc ctccacagcc atctcttcct gtttttcctc caagaagaac
    16141 gattacatct cctgcttcag gtctttctct tactacattc tcttttggtg ctgctgctat
    16201 aactgcacct atttccattc tctttgcaac aaagttttca tcatatactt ctgcaacttg
    16261 tccagttgta agacctattt ggttaccata tgaactatat ccatgagcag cttctgtagt
    16321 tatttttctt tgcataagtt ttccaggtaa agtatcttct aaagtcgttc ttgggtcagc
    16381 acttcctgta acacgcattg cttgataaac ataacttctt cctgataatg ggtctcttat
    16441 agctcctcct aaacaagtag ctgctccacc aaatggttct atttcagttg gatggttgtg
    16501 agtttcattt ttaaacatta ctagatattt ttccatctta ccatcaactt caacatctac
    16561 gtttatacta catgcattta tttcttcact ttcatctagg tcatttaatt tcccattttt
    16621 ctttaattct tttactgcaa cagtggctat atccattaaa catatatctt tatgtctgtt
    16681 cacatataca ttgtctcttg agttaagata catttgatac gcttctttta ctatatcatt
    16741 aaacttacca tcttctatct ttacattttc tatttcagtc ataaaagtag tatgtctaca
    16801 atgatcagac caataagtat ctaatacttt tatttctgtt atagttggat ttctcttttc
    16861 tgtatcttta aagtattttt gaacgtgttg taagtctcct aaagtcattg ctaatccttt
    16921 ttcacttaca aatgttctta atccattttc atctaaatct ataaatccat ctaatacttc
    16981 aactgttgtt ggtatttcag tttccatttt taacgtttct ggtttctcta aagatgcttc
    17041 tctactatct actggattta tacagtaatc ttttatttta gaaaactctt catcagttat
    17101 tttgcctgat aatatataaa cttttgctgt attaatagca ggtcttatcc cttgatttac
    17161 aatttgtaca cattgagctg cccaatcacc tctttggtcg tattgaccag gaagaaattc
    17221 tattgcaaat actctttcat catttaattt tggtatttct tcataatata ctacatctaa
    17281 atttggttca gagaatattg tctttgctgc attttcataa acttcttctg atattccttc
    17341 tacatcatat ctatttaata ttcttaaatt ttctatatta tctatgtgta aactttctac
    17401 taagtctttt tttaaagctt tagcctcaag gtcaaaacct tctctttttt caaccaatac
    17461 tcttctaacc attgagtctt tattttcagt attcaacata ccttatcctc cattttagaa
    17521 aaattatctt cctatgtctg ttctatagtg cattccttca aagtttatct tctttatgtt
    17581 ttcataaact ttttctgctg catccttaac tctagttgac tttgcagtta taccaattac
    17641 tcttcctcca ttggtaacca aattaccatc aaacatctta gtgccactat ggaatactac
    17701 tatatcatca tccaagtttt caagaccagt tataattttc cctttttcat aactgtctgg
    17761 ataccctcct gatgtaagca taacacatac agcttcatca tcactatagt ttatttctat
    17821 atcttttaac ttattatcta gtatagcttc cattatctca tgtaaatctg tttctaatct
    17881 aaataaaaca gattgtgtct cagggtctcc aaatcttaca ttatattcaa gtactttttc
    17941 tccatcttca gttatcataa gaccaacaaa taaaatacct ttaaaattaa gtccatcttt
    18001 tttgaaacct tctaaagttt tttctaatac tgtctctttt actttatttg ctaattcttc
    18061 tgtatatatt tcactaggtg aaaaagttcc cattcctcca gtgtttggac cttgctcata
    18121 gttgtttact tttttatggt cttttgcact tgccatagga actattgtat cattgtctac
    18181 aaatgctaga attgaagttt ctattccttt taaaaactct tcaattacta ttttatctcc
    18241 agcagctcca aacttcttat ctgacatcat ttcttttaaa gttgctatag catcttctct
    18301 attttcaggt attacaacac cttttccagc agctaatccg tccgctttta ttactactgg
    18361 atatccaaaa ctatctattt ctgatatagc ttcttctaag tttgtatatt ctttatattt
    18421 tgcagtaggt atgttatgtt ttatcataaa ttcttttgaa aaagctttac ttccttcaag
    18481 ttgagcacat tttttattag gtccaaatac tcttaatcct tctttttcaa actcatctac
    18541 tattcccata actagtggta cttctggtcc tactattgtc agacctattt cattttcttt
    18601 ggcaaatttt aaaagttctt ctatattagt atcacctata tttacacatt ctgctacgtt
    18661 tgcaattcct gcattacctg gtgcacaata aattttttct acattttttt ctttgcttag
    18721 tttccagcat atagcatgct ctcttccgcc accgcctaca accaatatct tcataatatc
    18781 ctccttagtg tttgaagtgt cttattcctg taaataccat aaccatatca tacttgtcac
    18841 aagcttctat tgaatcttta tcttttatag agccacctgg ttgaactact gctgatattc
    18901 catattcatg agctaatgtt acacaatcat caaatgggaa aaatgcgtct gacgccaaaa
    18961 cagctcctgt aaaatcttta tctttgttat tttcaagagc attttttaat gcccaaattc
    19021 ttgatgtttg tccacaacct aaagctagag tttgtccatt ttttactact gcaattgcat
    19081 ttgatttcat attttttact actctcatac caaattccat gtccttaagt tgtgcatctg
    19141 ttggtttttc ctttgttaca ttttcacttt tttctgctaa tatattgttt ctatcttgta
    19201 tcaataattt tccatctaaa tatttcattt catatggttg taaactattt tctattttag
    19261 ctaattttaa tattcttaga tttttctttt gtttaagtat ctctaaagct tctggttcaa
    19321 aatcatatgc tactactatt tctaagaata tttcatttaa tttctctgct gtagctttat
    19381 ctatagtaga agttattcct actattcctc caaatattga aaccttatcc gcttcataac
    19441 attttgtata tgcttcaaaa gcatcttttc ctaaacctac cccacatgaa ttagcatgtt
    19501 ttatagctac tgaaactact tcttcactat ctttaaattc tctcattact tctaaacatc
    19561 catgtaaatc atttaagttg ttaaaagaaa gttctttacc gcctaattgc tcataattta
    19621 agattggatt ttttgcattt gactgtgaat ataagaaacc attttgatgt ggattttctc
    19681 catatcttaa agtttgttct ttttggaaag ttaaatttaa tatgtcagga tacgtatctc
    19741 caacttctcc agcaaaataa ctagatatta acgcatcata tcttcctgta gttgagaaag
    19801 ctttatatga taattttttt ctgtcttcta atgtcattgt atctgttttt aatttttcta
    19861 ttatagaatc ataatctttt atatctacta ctactattac atccttatag tttttggcag
    19921 cagaacgtat catagatggt ccaccaatat ctatattttc aattatttca tcatgacttt
    19981 ttccagcttt taaagttcct tcaaaatcat atagatttac agctactaaa tctattgaat
    20041 gtattttgtg ttcatttaca gtttctacat gagattcttt atctctttta taaagtatac
    20101 caccatgtat atatggattt aatgttttta ctcttccatc taatatttct ggaaaatttg
    20161 taacatcttc aacttgcatt acattaacac cattttcttt taatgtttta aatgtatttc
    20221 ctgtagatat aatttcataa tctaatttat ttaactcttt agcaaattcc actacaccag
    20281 tcttgtcagt tacacttatt aaagctctct tactcattta taaacactct cctaccttga
    20341 agttttaatt tattttcaca aaaaagactt atactttctt ttaaaatcct atgttcaact
    20401 tctaacactc tttttgcaag tgtctttgca tcatcatctt gatttacctt aactacatct
    20461 tgcattatga ttggtccagt atcagcacct tcatctacaa aatgaactgt agcaccagtt
    20521 actttagctc cataatctat aacaccttga tgaacttttt ctccatagaa tccagctcca
    20581 caaaatgaag gaattaatga tggatgtatg tttatcattt tgtttctaaa ctcgttaacc
    20641 agctttgggc tgattatttt taaataacca gccaaaacta ctaaatctat tttattttcc
    20701 ttaagaattt ctattatctt gtcttcatca gtttcacaaa ttgcttttat gttatgattt
    20761 ttagctctct ctagcccata agcaccttgc ttacttgaga taactacttt aacctgacct
    20821 tttatttctc cagactcagt accatctata actgcttgta aatttgttcc tccaccagat
    20881 attaaaactc ctatatttag cataattcta caccttcatg actatctact acttcaccaa
    20941 ttatataagc tttatcttct aataattcac tatatttttt atctacataa gcagcattgt
    21001 cattttctct attatttata aagtttacaa catcatcagc attttcttta tctactatta
    21061 aaactagccc aatacccatg ttaaaactct tatgaagttc tctctcatca acagcattaa
    21121 aaccttctat cattttaaat ataggaggct tctcccaaga ttttttattg atatctagtc
    21181 ctaacccttt aggtataact cttgttatat tttctataag ccctccacca gttatgtgag
    21241 ctatagcttt tatgtcatgt ttagctaata catctaaagc taatttaaca tatattttag
    21301 ttggtgttaa aagtgcttct ccaactgtca ttcctagttc ttcaacatat tgttccattt
    21361 tataattgta agtttctaaa aatatctttc ttataaaaga aaatccattg ctgtgtactc
    21421 cacttgaaga tataccaacc aatacatctc ctgatttaac attgtttcca gaaacaatct
    21481 tttctttgtc tgctattcct acagaaaatc ctgctaaatc atagtcatct tctccataca
    21541 ttccaggcat ttcagcagtt tctccaccaa ttagtgcaca accagacatt ttacaaccat
    21601 ctgcaattcc accaactatt ttttcgatat gctcaggcac taatttccca agagctatat
    21661 agtcaaggaa gaataatggt tttgctcctt gacaaattaa atcattcacg cacattgcaa
    21721 ctaaatctat tccaacagta tcatgcttgt ccatcatctg agctattttt aacttagtcc
    21781 caacaccatc tgtagatgca agcaaaactg gttctttcat acctacaaaa tcttttaaac
    21841 tgtataatcc actgaagtta cctaaatctc caactacatt tccatcataa gttcctttta
    21901 ttttaccttt tataaggtct actgctctat ttccttcatc tatatctaca cctgattctt
    21961 tatatgttaa catgaaagta gttcctcctt atcaattttc ttaactgggt attctccatc
    22021 aaaacaagct ttacagaaag tagacttatg ttctgttgca tctaacattc cttctatatc
    22081 taggaatttt aaactatcac atccaatata ttctctcatt tcttctacgt tgttacttga
    22141 tgcaattaat ttagagcgat ttggagtatc tattccatag taacaagagt aagctactgg
    22201 aggagaagtt attctaagat gaatttcttt tgctcctgct tctcttaaag attttactaa
    22261 ttgtttagat gtagttcctc ttacaattga gtcgtctact aaaattatag attttccttt
    22321 tattattgtt gaaagaggat ttaattttat ttttactgct atttctcttt cttcttgtgt
    22381 aggttttata aatgttcttc ctacatatct atttttaact aagccttcac ttatttttaa
    22441 tccactagca tttgcataac caatagctcc tggccaacct gaatctggaa ctggaactac
    22501 catatctgcc tttacagttt cattctgagc taatctctca ccacatttaa ttctaaattc
    22561 atatgcattt acattgtcta tagttgcatc atttcttgca aagtatatgt gttcaaatat
    22621 acaacttttc ttaactggtt tataattttc tgaataaaaa tatgatttta gttctccatc
    22681 tttgactact attatctctc ctggctctac atctcttata acttctccgc caagtatatc
    22741 tattgcacaa ttttctgatg caaatatata ttcatcaccc tttttaccaa gtacaagagg
    22801 tctgaatcca tgtgggtctc taactgcaac caattcttct tgactcatta ttacaagagc
    22861 atatgcacct tttatataat ccattgtcac ttttaaactt tctactatat cccctttgta
    22921 atatctagca agtatatata aaatcacttc tgtatctgaa ttagtttgga acatatatcc
    22981 atcttcttct agcatatctt ttaaataatt tgcattaact agatttccat tatgagctat
    23041 ggctaattgt ctttttctac aacttccaac taaaggttga cagttagaaa catgacttcc
    23101 tccagctgtt gaatatctaa catgtccaat ccctatattt ccttttaact tagataattc
    23161 attttcctta aaaacatctc caactaatcc catatctttt ttgtacttaa tttctccatc
    23221 atctaaaaga gctaaaccac agctttcttg ccccctgtgt tgcatagaat ataaagaata
    23281 atatagttct ttagttacat ctttattaga gtaaatccct aaaactccac acatacattt
    23341 gcctcctatt tattattcat tcttgaaagt acttccgtat aaccttctac aaggtctcca
    23401 aggtcttttc taaatctgtc cttatctaac ttttcatttg tatttacatc ccaaagtcta
    23461 caagtgtctg gtgatacttc atctgccaat attatatttc cttcagaatc cttaccaaat
    23521 tctattttaa agtcaactaa ctttaggttt aattttaaga aaaatgcttt tagtaactca
    23581 tttattttta aagtttcttc tcttaagaat tttaactctt ctctagttgc taacttcatt
    23641 gccactgcat aatcatcatt tagcattggg tcaccataag catcattttt gtagcttatt
    23701 tcaaaaattg gctcatcgaa aactacacct tcttcaagac caactctttt acagatagaa
    23761 ccagcagcaa tatttcttac tattacttct aatggtaaaa tctctacttt tttaactaac
    23821 atttctctat ctgataaact ttttataaag tgagtattta tattattttc ttttaacatt
    23881 tcaaagatta ttgttgatat cttattattt agtatacctt ttgaagatat ttctgctttt
    23941 ttttctccat taaatgctgt tgcatcatct ttataataaa caacatattc attttcatta
    24001 tctgtagaat atacttgttt tgcttttcct tcatataata acatttctgc taccccctta
    24061 aattcttgtc gtcttctaac accttttgtg ccatctcttc tctatatgat ttcaaatcct
    24121 cttttaattt tgggtatttt aaagtcatta tttgtaatgc caataaagct gcgtttaaac
    24181 ctaagtctat tgtaactgtt gcaactggga ttccttttgg catttgaact attgataaaa
    24241 gtgagtctag tccatccata gttgatgatt taataggtaa tcctattaca ggaattaatg
    24301 tttgtgaagc tattacacct ggtaagtgtg ctgcttttcc tgctgctgct attattacat
    24361 cagtatcatc ttctatttct tttaggaata ttgaaagttg ttctggtgtt ctatgagctg
    24421 ataatgctct tgctacaact tctattccat atttttcaag cagttttatc ccttcttcta
    24481 attttggata atctgatttt gaacccataa ctactgctac tttcattaga cttcccctct
    24541 ccgtttataa gttattttac actacaaatc attcgtatta ttatacgaat tatacacctt
    24601 aaattttata ttaatataca tatattatca actctttttt gaaatttttt aaaaaaaata
    24661 tttcttgtaa ttttttcatt cgctacgtat cgaactttat ttgtgttttt ttgttaaaat
    24721 aaaaaacaga ttatctcaaa attggttttc taattaaatt ttaagcccaa tactcttttt
    24781 taaatcttgt atattatttt ttattacttc ttttttaatt aatactttgg atttatagtc
    24841 ttgtttttta gttctgaatg ttacttttac ttagataaaa ccaacacaaa gtttattctt
    24901 tatataagta atagctaaaa tttaaagtaa tgatattata taaatagaaa taattttatt
    24961 taaaggagaa tctgtatgtt agaagttata aaaaatagac gttcaataag aacatatata
    25021 gataaaaata tagaagaaga taaaataact gaaatactaa aatcagctat gcaagcacct
    25081 tcatcaaaaa atgctcaacc ttgggaattt attatagtag atgataagga actgctgaaa
    25141 cagctctcaa agtcacagca tagagcaaag catatagaat ttgcaccatt atgtatagta
    25201 gtgcttggaa atagagataa attcttaaaa cctggtaaat ggatacaaga tttaggtgct
    25261 tgcacacaaa atttattatt agaagttact aatcaaggtc tagctgcttg ttgggcagga
    25321 gtatttccta aaaataaagt ggtaaataaa gttagacaaa ctttagactt accattaaaa
    25381 ttagttcctt atgcacttat atctattgga tatagtgaag aaaaaaatga gtttatagac
    25441 cgatttgatg aaaataaaat acatagaaat gtatataaaa atagataatg tttagtaata
    25501 taaatgaaaa tacatagtaa aaatgccttt agaatacatt tgtattctaa aggcattagc
    25561 tttgactaaa cacctaatta gcctctaagg taaaatttaa ctatcattta ttatttatat
    25621 accaattgaa tattatatcc ttcaatattt ttatttattt caaatatatt aagaatatcc
    25681 tacaattaaa gttagctatt tttcattaaa gtattttatt gacacactcc ccatagctaa
    25741 agcaagggga ttcttggtgg ctaacacact tatttatact taaacagtac aacagaggtg
    25801 gttacaaagc caagctactt cggacggtgt cttcgccact actaccttaa agttattcgc
    25861 ccacacatgg ctcaaacggt agatactttt aaatttattg gttgcttatg atttattcta
    25921 aaatctacta aaccatattt atgcttttta tttaaaatat tccaagcacc aactatatct
    25981 ctatgagttt cataaccaca agtacaacta taagttctac cagttggttt gtgtttttta
    26041 gaacactttg ggcaagtttg agaagtgtag ttttctttaa ctaacttaac ttcaatacct
    26101 tgctcttttg ctttatattt tatatagtcc tttattcgac catactccat ttgagatatt
    26161 ttctgtctat taactctgtt taatcttttc tttttcttag tatctttttc aacccctttt
    26221 atatccccaa gaactatttc acataccttc tgctctacac aaaagtctat aaactttcta
    26281 gttgtttgat gatataaatt cattaactga ttatctgttt tacttcttat cttatttttt
    26341 aatctccata acttctttga ttgtctacta ccttttttac atttacttaa tcttttagat
    26401 atataagcat acatcttatt tctaaattgt ttaatacttc tgccttctct attagataga
    26461 ataagcccta caccttcatt tgttacacta gctatcgaat gtatttcccc taagtctaca
    26521 cctacggtat tagtgccttt ggctacaact ccatttaatt cattgtcaaa agtgtagtat
    26581 aaataatatc catttcgcca tactatttca gcagttttta tattctcaac attaaaatct
    26641 aattctaaat acctgccttt tgacattgat aattttaagt tgcctttagc atttctatta
    26701 atagcactag ctttaaaagg tattatataa aatttcttag tcttgtatgg atattttgct
    26761 ttagtattac ctttttttct taattctgat atagtctttc tatttgcatc aaatttatct
    26821 gatatagctt gaatagtttg actatgaaga ttgtacttac ctttgattaa cttttgtata
    26881 tcattctttt taatccattg tttatgttca aaatagtatt ctttagataa gttaactatg
    26941 tagttccaac agttagcact ttccatttgc ttttccctta ctaattcaaa ttgtgttcta
    27001 gttaggttta atttcactct atgagttttt atcatttctt tgtcctaact tgttgatttt
    27061 ctatatattg tttaactact tctaaactca cagaaccaac agtacctaca aaatagctat
    27121 tagtccacat gctaggcata ctagacttta aatgtggaaa gttctctctt aatactctcg
    27181 aactaaagcc ttttaaacct tttactactt tatgaattcc atattgaggg tcacaagata
    27241 ttaataagtg cacatgttct ttatcagttt ccatttctat tatttctgct ccaagttcat
    27301 ctgctttata tggtagtatt tcttttaaca tcttttcaac ttcgccaact aaaacaggtt
    27361 tacgatactt agggcaaaat acaatatgat atttgcaatc ataaactata tttttgttag
    27421 atttatacat aaaaactaac acctcctaaa tatagggtat cagtagcggt attagattac
    27481 aagcattatt ctaacttttt ttatattaag accttacctt tcatccctac acctaaagga
    27541 gagggctttt cggcaatttt attgtaaatt aataatgata atcacaactt taagaaaaat
    27601 atagccctta atgattatca taaaaattaa ttttttgaaa aaactcatat tgactcttga
    27661 agaagtacat acaaataaat tttgctttat gaatattcaa ataatttatt ttatagatat
    27721 ttaatgataa atatcctaca aaattattat taatatatta ggtcgactaa attttgatat
    27781 aaagagaata taaaatactg taaaaaagtg taatggttat ttccttgaaa agaaagacat
    27841 actattaaag cataaattac aatataggag atgattaaat gagaatactt tcagaaactg
    27901 aaaaaatatc tttagcagca gttataaaaa tggagtctga cggattactt atgcaaagag
    27961 caataaatat gttaatatca gatgaagacc taaaaagaca atcagaatct agtatattag
    28021 caacagaagg aagaattaaa gctatgcaac aatttatagt tgaaaatgaa atactaattt
    28081 ctgaggaggt atagataaca tgagaaatat gatagaaaat ctaattaaga acaatactta
    28141 tatagacgat aaagtaataa tgttaagtat gctttcatct gctaaagcat ctgccaatat
    28201 gtatcttaat tctgcattga caagtagtac acctgaatta agagctatat attcagcttc
    28261 attaactcaa atggtagaag gtcacacagc acttacagaa ttaagtatta ataaaggatg
    28321 ggttaaacca tatgataaac ctattcaaca gttaacatgt agttataaag aatcccaaaa
    28381 tgttattgac taaactaaat aaaatatatt tctaactatt tcattttaat taaataataa
    28441 aaaggggata ttcaaatttt tttaagattt ccccttcttc taaataatct atttttaaca
    28501 taaaatagat tgctcacaat ctaaacatat aattttatat agttattatt aattatttta
    28561 tacaattcta ttattaacat ttttttacta catattttta taactacata tttttataaa
    28621 taaagatatc aaatccttat gttccaaaat tacaaaatca ggtttgtaaa acaactcttc
    28681 tacctcatgt cctctatggt tcaaccatat actcttccaa ccagcttttt tagcacctaa
    28741 tacatcattt ctataagaat cacccacata atatgtattt tctctatcta aattcataac
    28801 attctcagct actctaaaaa tattagtatc tggcttagaa aatccaacct cactagaaat
    28861 aaaaatatta cttttatcaa cccagttttc aatatttaac tgctttaact tcattctttg
    28921 atggtttgat gggccattag ttataattcc tagattaata tttctttctt tagaaaaatt
    28981 taaaatcctc tcaacttctg gtattagtgt aatttgtgat tgttgatata tatactcatc
    29041 ttgaaaactt tgtgcatctt tttctgtaat agaatttccc aactcctcaa aagctttcat
    29101 tatcctataa atatgcattt ctttgattgg catttctcca ttttcagtca tatgaaatac
    29161 ctcatcacta tactttctac tactcatata taaatcttca atagaaatat cctttattga
    29221 agaaaacact ttattatatg cagtgtaaaa aggcgttaat tgattatata aagtatcatc
    29281 tacatcaaaa attaaattaa tcaaaatatt agtcctccta aatttaatag tggtaagtat
    29341 aattataact ataaatacct agtataacaa tactcaccac tataagtcct attatttatt
    29401 taaaatcctc gtagtaacac cttttaagtt atagcattct gcataatcac gcaaaggacg
    29461 tttaatatgg tctacaacaa atctttgacc atcaatatta ttcttactta aattatcata
    29521 aagcacttct atttcttttt taacatcatc attattaaat gtataatgtc ctgcaatatc
    29581 tagtatttca ttagataaaa cttcatcctt aaatatctct tcagtagtta gatttttttg
    29641 ttctcctact acccattttc tccaacgtcc acattcaatt gcattaacaa gaagaacatg
    29701 tctgatatta gaagttttct caactaagcc ctctttttct aatttacact caacttctgc
    29761 taattttaaa tatgccctag tttcttcagt accatattga ggagctacat ttgtagcaat
    29821 aatttgagat ggtatatgtt ctaataaagt tacatcatct aagtaatcgc cattatgctc
    29881 ttttaaacca actccatatt tcttagccat ttgagctaaa tcataagcat ttctaaaatt
    29941 aaaagttcct acttgttctg tctttctagt taaagtacca gtttgaccaa ctataaatgt
    30001 tggcattggc aaacctctat tatcgagttc aacctttagt ctagttataa atgtttcata
    30061 cgtctcagtt gaagtcagac ctccattggt ttcctctgta ccaacctcat aacctatgtc
    30121 tggtagatta agtctttttc tctcgttttc acaatactca attaattcta cagttctttc
    30181 tagtactaca tctagtggaa ttacttttcc aatttcaaat gggtcttttg tagggtcaat
    30241 catcaatagg tcaaaacctg cttcaatgtc cgctaaataa gatttttttc ctaactccat
    30301 tgcttcatca acaggcaaat ggtcatttct ctctttatct ctttgccatg gcccaccatg
    30361 gtctctacat aaataatata aaccatcaaa tccaactttg tcggcaacct tcttaatatc
    30421 ttttgtaaaa gtttcctgat tccaaccatt tacataaccg ccacctagtt catctgcatc
    30481 tacttgattt ctacttgcga taaacattaa aggaaaatca tcatcttttg ctagttcaaa
    30541 acttgcttgt aacaagtttg gtgacatagg accaattcct aaaagagttg cacttttccc
    30601 ctcttcttgt aagtttagta atctttgcac gacagtcttt attggtaact ttttcatgac
    30661 tatatctcct tttattctat ttttaagata ttttcaaaga tgcttttaat aaaaatttct
    30721 ttaaaaacat ctttgagtta tatgtaaaat aaatcaattt ttattgaaaa gtatctttta
    30781 ttttcttaat taatgcatca gatttcttaa ttatctcaga tacactcatt cttactacct
    30841 tctttccttt aaaacgttcc atatttctaa tctcaacatc attagtcaat attacacaag
    30901 ttgcttcatc aatatcttta agtgacaatt cattatctat tccagagctt ccttgagtct
    30961 caacctttat ttctatacca aattttttgg ctgataattc aagtgattca gccgacatgt
    31021 aagtatgtgc tactccacta ggacaagatg ttactgctac aattttcata ttcttatctc
    31081 ctttaaattt atagttttat atttactttt ttatacaaat tccttccatt tatatttata
    31141 attcttcaaa tattaattca attgattcat ctaaatcttc ttcaatttct tcatcttctt
    31201 tcacgtcttt ttttactaat ttcattaata ccgcaacaac tactgctcca gttaatactg
    31261 atgcaatata ccccaaccta ttgtcaacta caggtaacac tatcagacct ccccagggtg
    31321 catgatttaa acatcctaat aaaaaagcca taatatttcc tacaatcgac ccagccataa
    31381 tacaaggtat tactctaact ggatctgatg ttgcaaatgg aatagctcct tctgtaattc
    31441 caacacatcc cattaatagt gaagctttac ctgaatctct ttcttccttg ctaaattttt
    31501 ttggaaacaa taatgtagct aatcctaaac caattggagg tatacaaatt gcaacgccaa
    31561 ctccacccat taaatatgga agtgtgtcaa cttgagtttg ggcaaaagta gcagcaacct
    31621 tatttatagg gccacccata tctgaggcta tcattcctcc taagattcca cctaaaggaa
    31681 ttttagaaac accagacatt ccatttaacc agtttgtcat ccatgtcata aaagaagcta
    31741 taggttctcc aattaaaaat acaattatac cacctgtaat caatgtacca actaaaggat
    31801 aaataaatat agaacctaaa gttctcattg taggagctaa tttaattttc tttaattgta
    31861 aaacaacaaa tcctgctata aatcctgcta tgataccacc tataaaacct gcatttactt
    31921 cttttgctaa atatgcacct atcatacctg gagctaatcc tggtttatct gccatactaa
    31981 atgctatata tccacctaat ataggaatga ataaagccaa gccagcagct ccaatttgat
    32041 tcagtttagc caaccagcct gtttcaggaa cagcagcttg tccacttaaa gttactgata
    32101 aagcaaataa tattcctcct gcaaccacaa atggaatcat ataagatgtt gctgtcataa
    32161 gatgtttttg caaaaacttt aaaattctca ttttaatctt cctcctctat tttttctttt
    32221 ataagattat aaacagtttg ttcatctgaa catctaattt gatttctaaa actttcatgc
    32281 atcaataaac gtgctaattt tgataaaatc tttaaatgaa gattattttc attgtctttt
    32341 gttactgata tcaaaaatat taaatccact ttttctcctt cttctgtcca ttcaatagga
    32401 gtatcaagct ttcctattgt aatacaagag tttttaacac ctacactttg actatgagga
    32461 agacctatac catgtccaat ataagtcgac aatgtttctt ctctctttaa aacatctttt
    32521 aaaaattgat ttttatctat taaatatcct tcttttgaca ctacatcaac taacgatata
    32581 atggcttctt cttttgaatt aacatgtatt ttaaatgaac attttacatt aactaattta
    32641 ttcatacagt gacctccttc tcatatttat gaattaaaac ttctgcttct tcttttgtct
    32701 ttgcactttc taacatagca ataaaattag tcttataaac cattttagtt agattaatta
    32761 ttgcaggaat ttgctctttt ttatctctgc ttgctaggca aaatatgatt ttaacctttt
    32821 tatctccaaa actaactgct tctttacata ttaataaact tatcgaactt actttgacta
    32881 atgaactatc attgccatgt agtaaagcaa attcgttatc ttttacagca taaaaaccca
    32941 cattttttaa aatatctata atattctcag catattgtgc tgatacaaaa tcacaattag
    33001 caagtatgtt tgaactaaat tttacagcat cttcccattt catatctcca tcaattatcc
    33061 ttatggtatc aatccctatt aaatcactta aattaagttg tctaaataat attctctcat
    33121 cactgtatcc aagttcttga catataatac ttaatactct tttttgggca tcttcttcta
    33181 aaaagtccaa cctttttttt attgacatat aattagttaa gacattcttt ctttttatcc
    33241 ctttttcttc aaacttcaaa taatcctctt cttgtaaaaa tggatttatc tcaataattg
    33301 ttttaggcaa gtctattact atctttgatg ttgttataat gaagtcaata ctttcccagt
    33361 ctttataatc ttgtaactta tatgatggaa tactctctac tatatttatc tggtaatcat
    33421 ttactaaact atcctttaac atagcaatta caccatatcc aaatccacaa actaacaata
    33481 tatttttgta gttattcctc tcatttctat gtatacttcc taagaaatgt attgccaaaa
    33541 gtattgtttc attttcattc tttatttgac ttaaaactct gtttttttga atagaaacct
    33601 tagtagcttc aagtacatat tgatatttat ctggtattac atgaagtaat tcctcataaa
    33661 tctgtatatt ctgcttgatt ctctctatca atggggcaat atgattgagt aatcctttat
    33721 aaagaatcat atcttcttta aagctaatct ctaatatttc tgacatattg taaattaggt
    33781 cttgaactat aatttctgta ctaatcaaat ccatatcctc gtttaaactt gcatatttat
    33841 ttgtaaaaaa tacaaaactc tttaacagaa aaatttgttc cttagtcaac tttttttgaa
    33901 taattgtttc aaattcttgg tcaaacttat tttcaaaaca cggctcactt aatgaagcag
    33961 attctaaagg atgtttttca tctgaatgta tataccatgt aaaaaggaaa atgtttgaca
    34021 cataccatgt aaaagattca tcatttaaag tccatcccat ttgcaataat aaattcatag
    34081 accaattata tatatcaatc aaataaatcc ctttgaatat tttttcaatt acattcacta
    34141 aatacttctc aaatacactt aaatcagatt tttccttacc aaaaagataa gaatacgatt
    34201 ttaatatgtt caatctttgc ttatatataa tgtcttcttc tccacttatt ttaaaagatt
    34261 tatcataagt aatagttatt tggtttcttt ctaattcact tgacaaaaca ttaaggtcat
    34321 ttttaattga acttctagaa acctgaaatt ctttacttaa tttttctaag tttagattat
    34381 ctattttaaa cattgatata attttcataa tagatactcg ctcagttaag gaaaatacat
    34441 attcgttatt atttatacat actacttgga agtcatcagg aataattaac acacctttgt
    34501 tcatcttttg tattactgac ttgtttaatg aacgcaaaac aatatttaaa ttatttatat
    34561 catatctaac ttttctttct tctatactaa gttgtttgct aatatccttg taacttgtct
    34621 tattttttcc ttttagaaaa ttcatgatga gtaacattct cccacttatc atattttccc
    34681 tccttccaaa ataattagag ttattaatat agcaaatttt atattattat gtatgtttca
    34741 gtttttatct aagacagtaa ctatctttct atattcagaa taatacaata aagctttagc
    34801 cacaacttac aacatttgaa aattattttt acaaaatctg caaatatatt tttattaaat
    34861 atataaataa aaaaggcaag ttatattttt aattatataa cttgcctaaa ttttttatct
    34921 tgtacattta ataatggata tttttaattt tattattttc atatgcaaaa ttttcaaagt
    34981 cagaaattaa taatggtttt gaaaaaacat aaccttgaat ataatcacat cccacatttt
    35041 ttaggaactc aacttgttct aattcttcaa ttccttcagc agttactttc atacctagcc
    35101 ttttgctaag ttcaataata gacttaataa ctaccttacc acggtctata ttatttttgc
    35161 taacaaagaa tgaacggtct aacttaatta tatcaacttt aaaatccttt aatatcccta
    35221 atgatgagta cccaaatcca aaatcgtcta tagaacattt aaatccatta gaatgaatat
    35281 cctctattat atctatagct tctggatttt ctaagaaaat agattcagta agttctaaat
    35341 ctatcagaca tgttggtaca ttgtatttat tgcatatatg cttatatttt tctaaaaaca
    35401 atttatcatt cagatgttga cgtgatacat taacagaaat tctcaacact tctttacctt
    35461 cttttatcca cttagaaatc aatttgcatg tttcttctag tatatataaa tctaacttac
    35521 atataaatcc acttttttcg aaaagaggaa taaaatcact tggataaatt atgccctttt
    35581 ctgaattttt ccaacgcaca agagactctg ctccactaat ttttccactt tttatatcaa
    35641 ttttaggttg taagcacata aaaaattctt tattttcaag ggctttttcc attaaatttg
    35701 aaatttcctt ttcagatatt aatcgttttc tttctatatc actataaaaa gaaaaatcac
    35761 tttgatgagt tttattaaag ctttttcttg ctgtatttgc atggtcttgt attgtaatta
    35821 aatcttcatt atgattttca attgtgtaaa tacctactga tagtcttaaa aagtatggat
    35881 tttccctatt tttattaaaa taatttgtgt cagcttctat tttatataat aactccaggg
    35941 cttctaacac tggtctattc ttaataagca aatagaaaag gtctgcatca tgacggcaca
    36001 caaactcttc attttcatca atgtttttat acaaaatatt gtaaagatgt tttagtgtca
    36061 tatttccctt ttcactaccg aaacaatcat ttattagctt aaaatctttt atattcattg
    36121 ctatcatcat ataagtacta tcaggtgatt ctgaaattaa aggtttgact agcatactaa
    36181 acttagttga atttatacca tttgttatac tatctaaata tgcaatattt tctatttgat
    36241 ttacatattt tcttcttata atatataaaa ttaatgttat aattccaata atacatgtaa
    36301 ttataaatgt cattgttatt gtaaaagagt ttaatttatt aagttcacca agtacagcat
    36361 cttcaggtac tatgagaaga aaatgccaat catttaaacc tcttgtaatt ggatgataag
    36421 ttaataagca ttgtacacct tttgatgagg ttattgtaat attccctgat ttatcatttt
    36481 taaaatcctc aagcatcttt tgtgcccaag gttcatctcc agaactctta tatttatggc
    36541 taaggtcctt tattaattca gtattttttc ctatgaaaag aggttttccc ttactattaa
    36601 taatttcaat agttcctttt ccattaaagt tatctatatt aagaatctca ttcatacttt
    36661 ctttattgga tacacctatt aaaatactag atacttcatg attatcatta aatataggta
    36721 cactataaag aacaaaaccc attgactcta ttacttctga gtattcttta ccttcaattc
    36781 cttttttata ggaaacagta ttctttaagt caaacttttt tccacttaaa aactcagctt
    36841 taccttctaa atctattatt ccaacatcat aatacttata actagtcttt cttttagata
    36901 aatactcata tcgactatca ccttcatatt gcttaataaa atctgccatt attcttaagt
    36961 cggataaaat aaatttgatt ttagaatcaa cattcattac aatctgtgta gatacatcat
    37021 ttaaatagtt tttagattgt ttattagata tattgtgtag ttttacggta ttaaaaacag
    37081 taattacaag gattgaaata ataagtgaac ataaaattat agacagaact aaactatttt
    37141 tcttatacct tgataatttc atttttttcc tccaaattat aaatctattt tttaatgtat
    37201 attaacactt acaaattttt ttactaaaaa tccttcacac tatattatag tattctattt
    37261 cttaatattc aagaatgcca tcttaacatt tcattaacat tccatagata atggttttat
    37321 taaataatta tattatatgc aatttgaaac tatatttgta ttacaattta tcactgtatc
    37381 attttttatt tatttttaaa ttttggccaa aaaatttaaa ttgcccttgc atatgacacc
    37441 atgtcagggt atatcatcca tttagagagg tgaaatataa atgaaaataa ataactataa
    37501 aaatcaaagc atcatcacaa atcctaaaaa atttgaaaat aaatatcaag atttaccaaa
    37561 gactccaatt gagttattaa aagtggttca aagcctagta attcatggag accaaggaaa
    37621 attatatggt atatccttta ataaacaaca atctgatgag gaattgcttc gtacaattcc
    37681 tcaaatgtta aaaagaatat ttgaaataaa ttctaaccca ctaactattc caagaaaccc
    37741 aaaacaaagg cttgttggaa tgtgcagaga ttattcttta cttcttgtat cgctcctaag
    37801 atatcgaggt tttgaggcta gaatgagagc aggttttgct aattattttg aaagtgagct
    37861 tacttatgaa gaccactggc ttgtagaata ctatgatact ctaacaaaga gatggataag
    37921 aatagatgca caaatagatg acatacaaaa aaattatttt caaattaatt ttgacactca
    37981 tgatgtcgga aaaacagatg gttttttaac tggttcagaa gcctggataa gatgtcggca
    38041 aggacatgct catcaagatg actttggata taataaaaat tggaaaggtt ggcattctgt
    38101 aaagggtaat cttctacatg actttaataa tatgattggt ttagaattgc ttccatggga
    38161 tttatggaca gaattaagtt ccaaaaaata caatcaatta acaagagcag aaaaaaattt
    38221 attagatgaa atggcagaaa tattatcaag cggaaatata aaaatagaag accttaattt
    38281 gttaatagaa aaattgcctg aagattattt gaaatcaatc ttttctcagt taaaaatatt
    38341 aggcatttcg gaaataaaag aattaggcaa tcctcttgaa ctcgaaaaga aattcaaatt
    38401 tacaaaatca ataaataaat caattaaaaa ttcactttgt cataataaaa gctctatcta
    38461 tttaaaagga ggaaggcaaa acaatttaaa ggatgttgag gttacaattc caaaaaatca
    38521 aataactgtt attacaggtg taagtggtag tggaaaatct tctttagctt tcgatacgat
    38581 ttatgaagaa ggaaaaagac gttattttga aaatctaagt aatggagcaa aactttcaga
    38641 acagcttcaa aaaccggaat ttgatttact tcagggatta acccctacta tagctatcga
    38701 acagaaaaaa ggaagccaaa atcctcgctc tactgttggt actcttacaa gcatttggga
    38761 ttatttaaga atgttatttg tatcaatcgg aaaaagttac tgtccatatt gtaaaattcc
    38821 attagaaaaa aagaataata caaaaaatta ttgtccacat tgtcaaacta ttttttctaa
    38881 gataaatact tcaaccttca atgctaactc ccatactgga gcttgtcacg attgtaatgg
    38941 attaggtttt acctatcaag ttaatcctca attgattgta aaagacccaa ctatttcaat
    39001 attagatgga gctacttact attttggaaa attgaagggg aaaaagccaa atggtaattg
    39061 gatggtaggt gaattatatg caatagcaaa agataaaaat atagacctag atataccttg
    39121 gaatgaatta ccaagagatt ttatagatgc aatcttatat ggtacagacg ataagatata
    39181 tgaatttagc tttgaatcaa agggacgaga atcaaagatt agaagacctg cttcaggagc
    39241 aatcaaccac attcaaagac tttttagaga atctagttct gaaaataata ccttgcatca
    39301 atatatgaac aaaattcctt gcaatacatg tggtggcgaa ttgctgtgca ttgaggctag
    39361 attcactaca atcaaaggtt atcgcttccc tgaactaaca aaaatgacga tagaacagtt
    39421 atggaattgg ttgtgtgaat taccaaatca acttcaaaaa aatgagctct ctttggtaaa
    39481 tgatatctta acagaattaa aaattcgagt tagttatcta ttaaaggtag gtcttagcta
    39541 tatatcaaca gatagaacag ctccaacttt atctggagga gaactgcaga gagtacgatt
    39601 atcaagtcag ctaggaagtg aattagtagg cttaacctac attttagatg aaccatctat
    39661 tggtttacac ccaagagacc ataatttaat catcaaaatg atagaagagc taagagataa
    39721 gggtaatact gttatagttg tggaacacga taaagataca atactgtctg ctgattatat
    39781 tattgatgta ggcccaagtg ctggtacaaa aggggggttc attattgcag aaggtacaac
    39841 ccaagaaatt ataaaaaatc caaactctat aacaggtaaa tatttaagca catacaataa
    39901 aataagttca caaaataaaa ttataccaag taaatggtta agcttaaaag gatgccatgc
    39961 aaataactta aaaaatattg atgtagaaat tcccttaaac tgtatgtgct ctattacagg
    40021 tgttagtggt tctggtaaga gctctcttgt atttcacagt ttacttcctg ctctagaaga
    40081 aaaattgaaa caaaaatcta tcccagataa aaattatact gaatttactg gatttgatgc
    40141 tattgatgat tttatcttaa tggaccaaac acctattgga aaatctagcc gttctacacc
    40201 tgctacatat atcaatattt ttgatgaaat tcgttctcta tttgctgaaa ctccacaagc
    40261 aaaacaaaaa ttattggatg aaagctattt tagctttaat agcaaaaagg ggcaatgccc
    40321 aaactgtcaa ggattaggta aaacaaaaat tattctgcaa tatatggcag accaatgggt
    40381 aacttgtagt gaatgtcaag gtaagagata tcaaaaagaa atactgtcta tacaatacaa
    40441 gggaaagaca attgcagata tacttgacat ggaagtagca gaagcaaaaa cattttttag
    40501 cgattgctct gatatatata gaaaactctc cttattagat gaagtgggac ttggatatct
    40561 aaagcttgga caaaatacat taggattaag tggaggagaa tctcaaagga taaaattagc
    40621 aaaagaatta gggacaaaaa ctaaaaaacg aatgctgtac atattggatg agcctactac
    40681 tggacttcat tttaaagata tagaaaattt gttaattact tttagaaaac ttgtaaatga
    40741 acagcattca cttttaatta ttgaacacaa tacagaggtt atacgtgctt ctgattggat
    40801 tatcgatata ggtccagaca gtggaataaa tggaggggaa attgttgctt ctggaactcc
    40861 tgatgaaata aaaataaatc caaatagtat tactggacat tttatataaa aacaattaaa
    40921 tacacatgaa cttaaatata aaattataga atgtcttata ttatagaaga attattataa
    40981 taggtgtaca ataaaaaaat taactttagt atattatctg tgataaaata tgaataatat
    41041 ataaaacaga acataaaaat tattatattt tataatagag aaaggaaaat caaatgagtt
    41101 ggaaacatat aaaaatacca aatggagata caatgatttt ttgtaactca tttattacca
    41161 gaaaatcatc catgaatatt gttgttacac atactcctat tgtcactacg cttgagatgc
    41221 aaaaagcata tgaaccactt gccaaacata atgtaaatat atttgctttt gatttttcag
    41281 gaacagggaa aagtgatgga tatgaaaaag atttttctcg caattcaatt gtaaaagact
    41341 tagattctgt cattacatac atagaaaaaa attattcttc caacatacat ctatatggga
    41401 atacgggaat tggtggtatg tttgcacaat attatgcatg ttcagacaat aagttaaaaa
    41461 gttttgctca gtttgcttgt gttgattata aaaacacaac aggagtaggc tatacctatc
    41521 tcactgttaa attattaaac tttttcttta agtttcttcc taattttcac ataacagtaa
    41581 aaccacctaa atataaaggt tataatgaag tctatgataa tgaattttat aaaatatttg
    41641 aaaagaaaaa tcctcatatt tggaaaaata gtacaaagat aattaataca atgttagaat
    41701 gctttattgc aaaagatagt aagataaaag atggagtcaa catacctact cttgtcttta
    41761 aaacacttca tgatagatat tttaaaaccc aatattttga tagctattat aattcactaa
    41821 aatgtaaaaa gaaacttgtt gaaattaata atgttcataa tagttactat atagatagtg
    41881 agaaattttg ccaagaagct tatgattggt ttgtagaaaa ccagtgatat aatttaaatg
    41941 ttatatgggt ttctgattat cttttcagaa tttattaaaa atccaaatgg ccttacttta
    42001 ttggctattt ggatttaaga tataaaaaaa tagatatcct ctacttacca aaaacttata
    42061 aataaaaaaa tattcccagt ataccactta ttattataat cacaatagga ttaattttcc
    42121 acttacgtac tatcagtagt gtaacaaaaa agattattag tgcagtccag tttagcatct
    42181 caaatataga attgaagtac actttattcg tcccaaataa tgcaaggatt ataattgtag
    42241 ccgcagctga gataattaaa cctaatgaag ctgattttag accatttaat atttcaaata
    42301 tataagtaga gttctgatgc ttttgaaaaa aattatataa aaatagagat attactacac
    42361 cacataatac acaacctaaa gtggctgcaa ctgctcccca aaaccctcca atttgaagtc
    42421 caacaaaagt agaagtatta acagccagtg gccctggtgt catttgtgaa atagttataa
    42481 tatctgtaaa cattttctca cttatccatc cagtttgatt tactactttc tcttggatta
    42541 aaagtatgat tgcataacca ccaccaatac taaacaagcc ttatctctac gacagttatg
    42601 cacgtttccg ttattgttac gtgagcaagg aagtgccatt cgcaatactt tggacagtgt
    42661 attatctctt tcaaaccaaa aagcttatcc tgtttgggaa agtgttaatt cttttgcact
    42721 tattaaagct gcacaagcag ggttaggtat aaccattttg ccagaaaatt tattattaga
    42781 ttcactctta cataaaaaat tgcgattgat tgaattagat gggatagata tggaaaataa
    42841 aatgttagca atattacata aggataaaaa tattacacaa cctttgaaaa taatattgga
    42901 taatataagc aatgtacttt aatttaaagg tttaattttt taacattcta ttttttagca
    42961 taaaaagtgt gtactattct ttaatgttgt gattttaatt atcatcaatt tttaaaataa
    43021 tacacacttt attttaagta caatctaaac ttaaataaga taaaagtttc tattgtatat
    43081 gttttaaatt aatactatat tcttgtttac ctttttcaat tttaaattta tcatttttat
    43141 taggataaat agttacaaca ttatctttat actctgattc atcacatttt atattaattg
    43201 gcataacttc agcttcacca tatacatagc taacaggctt attattattc attacaacaa
    43261 tctgcaccat gtcatcaccc tttggagatg atatatttct ccatttatat cctattgttt
    43321 tatatatatc atctgcagat gttccagatt taaaaacata tattttatcc cattcaggag
    43381 tctttacttc tgataatctt atttctttct tatctttact tactttatta attgaagaaa
    43441 taaattctcc aaatttatca ctttcttgat ttttttcaaa tttaattaat ttactagatt
    43501 tgtcaactat attccattta tcattgtttt tttctaattt aaaattaatc ttagaaatac
    43561 attccccatg ctttcctggc tcagtaacta taacattttc tcctgattta tttttataat
    43621 catgttgctc aatttgaaca tgattatgac ctgcaactat agcatcaatt ccatcaactt
    43681 gttgagctaa gtcttgtatt ctatttcctg ggtttttagg tttcttaggt ttctcacctg
    43741 tatgtgccac agcaactata atgtcaggct tctctttttc cattatacca acccactttt
    43801 tagcatcttc tactaaatca ttcatataca attcaccatt gtaaccctct tggtctttta
    43861 gttctaaaga cttagagtca acaagttttc catctttcca ctgtttcctc tcaccaactt
    43921 cttttatagt aagccctaaa attcctagtt ttacgtttcc ttcaggtgta ttaatatttt
    43981 ttattatgta tggttttgta taactatctc catttttttt atacgtattt gccgataaaa
    44041 tatttatttt ttgcttctca aaatcagata tcatattatc tagataaaat ttattgttgg
    44101 atataaattc atgattccca agaactgcaa catcataacc tacctctttc atatcttttg
    44161 aaataggcgt ctcaatataa ctttcagtgt tattatcacc atctttatat ctagcactaa
    44221 aataactatc catgctagaa ccaaacactt ctccactatc aaaaaaatct cctgcatcta
    44281 ctaaagttag atttttatct ttttttcttt cttcttttac ataagaagtt aactcataag
    44341 gtataactcc atgtaaatca gtagttgcta atacgtttat ttctgcggtt tgttttgaag
    44401 cacatccaga cattagtaat acaagtgaaa aagtaattcc tataattatt ttatttctca
    44461 taatccctcc agcttctata aatatttgat ttagtatcat tgacaactca attcttctat
    44521 aatatcaata ttcatacatt aatatacaaa agtcatctat atgttttata gacatacatt
    44581 tcatagacat aaaatacttt tatatacttc taataattat aaatcttttc tattatatca
    44641 tcaagtttat ttatgattat accaaatggt tttctaatta agtgaatata aaaagctatg
    44701 aacaatatta ctcaaattac catcaagagt aacataaaca catatctcca tcaatgatta
    44761 agaaattact tttttattgt atcaatttgt ctttcagagc atgacacaaa aggaactaaa
    44821 agataacaaa atcatcagtg ttaaatactt cgagtttcag agcaaaggag ttctaaaaca
    44881 aaataaaaac accccgctga atttatcatt cagcaggatg ttttttgctt attaatccat
    44941 aagatactgg caaatattga tagtatcaaa ccagtataac atagaagtaa gagagacctc
    45001 aaatcctgta tttatgtgga tttttggcat tctatatttt attcccattc tattgatttt
    45061 ctttttaatc tgataaaacc ttatctatta cattaatttt cttctattat ttctatattt
    45121 tctatatttt ttaacacgct ataaaacata ttagtatcat tttagtatca cattcatttt
    45181 attcatcttc ttcccaattt ttcacccatt cctctaacat atctaattta tcatcactta
    45241 aattttcttc atcatttaaa gcagtgaaaa aactttttaa agaattatta tgaaaaaaac
    45301 taaagaattt ttttgtttca acttttaaat aatctttttc tcttacagcg atagtataat
    45361 atgtacactt gcttgttttt tgagaataca aatagttttt ctttgcaagc ttagacaata
    45421 ttgttaatgt cgtattttta ttccagtcat aaatttgttt cattgcttcc acaacttctc
    45481 ctgatgctac cttatttcca tctaatcccc aaataaactt cattactttt aactcagaat
    45541 ctggcaattt cttgaatccc ataagctaca cccccttttt gtaaatttat gtatgtttat
    45601 gtatatttaa atattataac aaatttattt taaaaataat taatatatat ataaattatt
    45661 cttttccaat tttttccata ttttctatat atttttttat ttgttcttct gtcatctttt
    45721 cttttttatg catagtggta aacattcgta taaaaaaact atcatattta gttacctttc
    45781 tatcttcttt tattgcattt aaatattctt ttttagttac tagcacttca taactaaact
    45841 gaaaacctat cctcttcttt tttaatgttt tcatttttac taatcttttt aatagaattt
    45901 ctgtagtact tttcctccac ttatattttt ctttcattgc ttctattatt tcttttttag
    45961 ataaaataga atttttcctc cacaagtatt ccatcaacat caattctccc ctgcgtagtt
    46021 tattaactac ttcttgttgt ttcattttat gtcactccct caaattgttc taatctaaat
    46081 caataataaa accttctgat ttcaatttat acaggtaagt tttaccaaat attctacgca
    46141 tttagtctgt tttcttagaa gaaaataact ttcatatcta taaaattatt aaattttcaa
    46201 acaattttgt tgaataataa ctaattctat tgtattattt tagttgtaga ataaaactaa
    46261 atcggcaaaa ctagagaaat ttagtgacgc aaagctatag ggactaagac ttatataaat
    46321 atcttatgag ttatgtcagc cagttgccaa agagatattg ttcttttgtt tttatgaaag
    46381 ttttttaggg ggataataat atatatactt agagataaaa taaaatatat ttctagcatt
    46441 cacagtttta ttttttctta aaatgcgata taataaagat gtaaattcgt atcaaaaaca
    46501 aaaaaagaaa ctacaatcta ttagcctaga gtgaagtttc attaactaaa tattaatgtc
    46561 gttttttatt aaacttgatt tttacatcaa gctcaaagtc actcctgcca gagtggcttt
    46621 ttacttttct ggaaataata tatgtaataa agctagctgt taaactagct aacactccta
    46681 gtaaaaattc gctcatttct ccacctcctt ccttttaagg gatggaatgt agaaaatgaa
    46741 gctccactct tagattgtag ttccacaaga ttattcttgc attttaatta taacataatt
    46801 ttacaattat caaatatcta ttctccattt tttttattta tacaatatat tctatattgc
    46861 aataaaaaac attgttttaa atatatatac ataggtatat actaaatatg ttaaacttaa
    46921 ttatggaaaa tataaaatga aaggaat
//
LOCUS       DACULQ010000008        46045 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.008, whole genome shotgun sequence.
ACCESSION   DACULQ010000008 DACULQ010000000
VERSION     DACULQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.008"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            1..219
                     /locus_tag="JA154_01870"
     CDS             1..219
                     /locus_tag="JA154_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994605.1"
                     /translation="MDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRDIFVN
                     RITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            276..926
                     /locus_tag="JA154_01875"
     CDS             276..926
                     /locus_tag="JA154_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994606.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEYIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1129..1800
                     /locus_tag="JA154_01880"
     CDS             1129..1800
                     /locus_tag="JA154_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7994607.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            1797..2927
                     /locus_tag="JA154_01885"
     CDS             1797..2927
                     /locus_tag="JA154_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7994608.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLIDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3358..4203
                     /locus_tag="JA154_01890"
     CDS             3358..4203
                     /locus_tag="JA154_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7994609.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4203..5231
                     /locus_tag="JA154_01895"
     CDS             4203..5231
                     /locus_tag="JA154_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7994610.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5234..6247
                     /locus_tag="JA154_01900"
     CDS             5234..6247
                     /locus_tag="JA154_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7994611.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVF
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(6764..8014)
                     /gene="cme"
                     /locus_tag="JA154_01905"
     CDS             complement(6764..8014)
                     /gene="cme"
                     /locus_tag="JA154_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HAT7994612.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8100..8846)
                     /locus_tag="JA154_01910"
     CDS             complement(8100..8846)
                     /locus_tag="JA154_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7994613.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSSPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(8999..9961)
                     /locus_tag="JA154_01915"
     CDS             complement(8999..9961)
                     /locus_tag="JA154_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAT7994614.1"
                     /translation="MSSEKLSQLEPLIISQYSNINGIIIARKGKIVFEKYYNGYNADD
                     TFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPEYTCSPSEICKKAVTIRHLLT
                     MTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIGTFKYSSAGTHLLSAIITKTT
                     GKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKNLKGWAKDRQGYSIGGWGLTL
                     SARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSNEYGYLWWLCKKDGVLWYSAM
                     GTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYILTSIID"
     gene            complement(10021..12441)
                     /locus_tag="JA154_01920"
     CDS             complement(10021..12441)
                     /locus_tag="JA154_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAT7994615.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSLKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLEQGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12527..13447)
                     /locus_tag="JA154_01925"
     CDS             complement(12527..13447)
                     /locus_tag="JA154_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7994616.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            14037..14963
                     /locus_tag="JA154_01930"
     CDS             14037..14963
                     /locus_tag="JA154_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994617.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            complement(15346..16998)
                     /locus_tag="JA154_01935"
     CDS             complement(15346..16998)
                     /locus_tag="JA154_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HAT7994618.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            17597..18718
                     /locus_tag="JA154_01940"
     CDS             17597..18718
                     /locus_tag="JA154_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HAT7994619.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILIPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            18754..19167
                     /locus_tag="JA154_01945"
     CDS             18754..19167
                     /locus_tag="JA154_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7994620.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            19160..19699
                     /locus_tag="JA154_01950"
     CDS             19160..19699
                     /locus_tag="JA154_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT7994621.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(19899..20384)
                     /locus_tag="JA154_01955"
     CDS             complement(19899..20384)
                     /locus_tag="JA154_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994622.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            20728..20874
                     /locus_tag="JA154_01960"
     CDS             20728..20874
                     /locus_tag="JA154_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994623.1"
                     /translation="MIIFWLLLYLAVIFSIGSMITRVNKVWVKVLLFIIVGLSTIVLS
                     YVIT"
     gene            21289..21666
                     /locus_tag="JA154_01965"
     CDS             21289..21666
                     /locus_tag="JA154_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089703.1"
                     /note="has endoribonuclease activity on mRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive intermediate/imine deaminase"
                     /protein_id="HAT7994624.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            22062..23867
                     /locus_tag="JA154_01970"
     CDS             22062..23867
                     /locus_tag="JA154_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAT7994625.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            23944..24481
                     /locus_tag="JA154_01975"
                     /pseudo
     CDS             23944..24481
                     /locus_tag="JA154_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            25095..26309
                     /locus_tag="JA154_01980"
     CDS             25095..26309
                     /locus_tag="JA154_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HAT7994626.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            26642..27829
                     /locus_tag="JA154_01985"
     CDS             26642..27829
                     /locus_tag="JA154_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HAT7994627.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            27845..29428
                     /locus_tag="JA154_01990"
     CDS             27845..29428
                     /locus_tag="JA154_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HAT7994628.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            29456..30784
                     /locus_tag="JA154_01995"
     CDS             29456..30784
                     /locus_tag="JA154_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HAT7994629.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            30929..32281
                     /locus_tag="JA154_02000"
     CDS             30929..32281
                     /locus_tag="JA154_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAT7994630.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            32473..33582
                     /locus_tag="JA154_02005"
     CDS             32473..33582
                     /locus_tag="JA154_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT7994631.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            33865..35256
                     /locus_tag="JA154_02010"
     CDS             33865..35256
                     /locus_tag="JA154_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HAT7994632.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            35250..37811
                     /locus_tag="JA154_02015"
     CDS             35250..37811
                     /locus_tag="JA154_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HAT7994633.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTEDALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEEDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENVVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            37987..39333
                     /locus_tag="JA154_02020"
     CDS             37987..39333
                     /locus_tag="JA154_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HAT7994634.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            40129..41151
                     /locus_tag="JA154_02025"
     CDS             40129..41151
                     /locus_tag="JA154_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7994635.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            41211..42218
                     /locus_tag="JA154_02030"
     CDS             41211..42218
                     /locus_tag="JA154_02030"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089691.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAT7994636.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            42440..43642
                     /gene="pgk"
                     /locus_tag="JA154_02035"
     CDS             42440..43642
                     /gene="pgk"
                     /locus_tag="JA154_02035"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089690.1"
                     /note="Converts 3-phospho-D-glycerate to
                     3-phospho-D-glyceroyl phosphate during the glycolysis
                     pathway; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAT7994637.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            43668..44411
                     /gene="tpiA"
                     /locus_tag="JA154_02040"
     CDS             43668..44411
                     /gene="tpiA"
                     /locus_tag="JA154_02040"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089689.1"
                     /note="Reversibly isomerizes the ketone sugar
                     dihydroxyacetone phosphate to the aldehyde sugar
                     glyceraldehyde-3-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAT7994638.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            44420..45952
                     /locus_tag="JA154_02045"
     CDS             44420..45952
                     /locus_tag="JA154_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089688.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAT7994639.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT        17623 a         5469 c         8193 g        14760 t
ORIGIN      
        1 atggatagta tgttatatgt actaatttat atgacattat tggtatctat cattggaagt
       61 atattttatt tttgtaaaga cctcttaaat cataaaatta agtatgtttt aataagagac
      121 atatttgtaa atagaattac atattctgaa gaacgactgc ctaaacagta tatagttttt
      181 cagaaatatg atatttggag gtattgtagt ttgttttaat ttgaaatgaa tctattataa
      241 attctaaagt ttaaaaataa aaaggagaga ataaaatgga tttcatttca agtatattgc
      301 aaggattatt aagtatatta tttaatttta caggtgattt tggaatttct attgtactta
      361 taactttaat agttaaatta gtacttatgc cactatcatt aaaacaaaga ttttctgtaa
      421 aaaaacagca agaattagct gaaaaaatgg aatatataaa agaaaagtat aagaatgata
      481 ctaaagaatt ggaaaagcaa atacaagtaa gttctgtaga aagtatgaag agtatgctgg
      541 gatgtcttat agttttggtt caaatgccag tagtatatgc tttatatcat gtatgtttag
      601 atatgacaaa ggaatttaca acaattataa ttccatggat tgctaattta ggtacatttg
      661 ataatttgtt tatattacct tgtatatata cattaattat gttagcttca aatttagtga
      721 attatatacc atatttaaaa ataaattctc aagtaaagtt taacaaacaa atggctgtgg
      781 tgactaccat aacaagtctt atactaactg ttagaactcc agtagccata ggtctgtact
      841 ttataacaag tgcaatttat tcattaattg aagatatttg ctttagaata tattttaatc
      901 gaaaaaatag aatagtgaca aaataatata attattatta gaaatatttt agcctgctaa
      961 catagaaaaa gttagtaggc tatttttatc tatgatatca attccattaa gaatagtcta
     1021 aaaaatacta tcaaaatgct ataaaaaata ctataaaaaa atagttgaaa aataaataaa
     1081 ttaatataaa ctaaaataaa gcctacaaaa aagaggtgat aaatagaaat ggctaaaata
     1141 ctagttgtag aagatgaaaa aagaatgcaa aatattattg tagaatatat gcaaaaggga
     1201 ggatatactt gtataactgc tgatgatgga gtcgaggcac ttacaatatt aaagagcaac
     1261 aatatagatt taatgatatt ggatattatg atgccgtatt tagatggatt ttcagtgtgt
     1321 agagtttcac gtgaaatgac taatatacct attattatat tgacagctaa aggtgaagag
     1381 gaggataaat taaagggtta tgaatatggc gcagatgatt atataacaaa accatttagc
     1441 ccaaaagtac ttttagcaaa agttaatgct ttgttacgta gatacactac tgatattccc
     1501 aaaaattcat tgagtttagg aaagattttt attatggtag catcaagaca agtgtatgta
     1561 gaagataaat tgattgattt aacatataaa gaatttgaat tattgcgttt atttatggaa
     1621 aaccctaatc aagttttttc cagagagaaa cttttaaatt gtatatgggg atatgatttt
     1681 gaaggaaaca cacgaactgt agatacacat ataaaaacct tacgcaaaaa gcttggtagt
     1741 gaaggacacc atatagttac attaattcgt tcaggatata agtttgaggt taaggaatga
     1801 atagtttaag aaagattata gaaaaactga aaaatgtaga tatttcaaat tggattaaac
     1861 aaaattttgg actatattca gttagaaaaa aagtgttttt actttctaaa ttatcaggag
     1921 cgttaattat attattttat ctggttgttg aagaattttc gataaactct aaatttgaat
     1981 tttggatttg gtttataata ctggttatat ttattatgag tattgatttc ttattgggaa
     2041 aatttatttc agaaccaatc acatctataa ataaatctgc aaaaagtatg tcccaacttg
     2101 atttttctaa tccatgtact gtaaatacta atgatgaatt tggagaactt tctaggagcc
     2161 tgaatacaat gtctacaaat ctacaacagg cattatcaga cctagaaagt gcaaatattc
     2221 aacttgaaaa ggatgtaaat aaggaaagaa tgctattgga acaaagaaag gagttggtag
     2281 atacaatttc acatgaaatg aagactccac ttggcattat acgtgcgtat acagaggggc
     2341 ttatagatga agttgatgaa gaaaaaaaga aaaattatat gaatgttata atagaagaaa
     2401 ctgatagaat gaacaatatg attgtatcat tattagactt atctgcattg gaagcaggag
     2461 tttctaaact taacccagag cgttttgatt ttatagagtt tgttgaaact gtggcaggtc
     2521 gtcttttaat tgatatacca gatgccaatt tttattttac atatgatttg ccagagtatg
     2581 aagtttttgt agtggcagat aaaatgagaa tggaacaagt agtagagaat ctagttatta
     2641 atgcaaagaa gcatgttgta tataatggta atttagattt atcgattaca tgtgaaaatg
     2701 gcttgctttt atttgaaatt tataatgatg gtaggtttat agagcaagat gagatttcaa
     2761 agatttggtc taagttttat cgtagtgttc aatctcagcg tacaggaggt tcaggtctag
     2821 ggcttgcaat tgtttcacaa atacttacaa tgcaagggtt agaatatgga gtgaataatc
     2881 gtgatagagg agtagagttt tattttatga ttccaattga tgaataattt agtattaatt
     2941 tataaattat aagtatctga tgtaaaatat ttttatttgt atacgtaagt atttaaagag
     3001 aataaaataa aaaaacttaa tgaaaaagct taatgaaaaa gtctaataaa aagcttaatg
     3061 aaaagcttaa taaaaagtct tgttttcctg atgaaaacaa gattttttat taagcttttt
     3121 attatttcac ttcgatttca caagagagac actctgattt caccaaatag aaataaaata
     3181 ggttaaataa attaaaaaat agaagcgtaa ttaaataagg ttacaagggg gagtacttat
     3241 gaaatggtat acatggttga ctattatatt gataatattg ctttctattc cgcttaaaat
     3301 taaatttata aaatggtggg atagacgaca aaaaaatgat aaaggaggtt atgatagatg
     3361 ataaaagtaa gagagttaaa attttcttat ggaaaagata aacagatatt gcatggactt
     3421 aattttgaag taaaagaagg agaaatcttt ggattccttg gacctaatgg ttcaggaaaa
     3481 tccacaacac agaaaatctt aaatggtgtt ttaaaaggtt acaatggtca cgtatccatt
     3541 tttggtaagg aagttaaagc atacacagaa tctttatatc aaaagattgg tgttttattt
     3601 gaatttcctt atttatatac caatttaagt gcaattgata atttagaata tttttcgtct
     3661 ttttatccta aaaatcaaag gagagacata tatgaactct tagatttgtt agagtttaag
     3721 aaagagttta taaataagcc tgtatcatcc tactcaaaag gtatgaagca acgtattagc
     3781 atggcaagag cattggtaag taatccaaag ttgttatttt tagatgagcc tactagtgga
     3841 cttgacccat ctggagctgt actatttcgt aagattattg aagaagaacg aaaaaaagga
     3901 acaactatct ttttgactac tcataatatg ctagatgcag atttaatgtg taacaatgtg
     3961 gcttttattg cagatggaaa gattatggta atagataaac ccaaaaattt aaaaatgaaa
     4021 aatagcaata ataaagttga agtggaattt gtatataatg gaaatcgaga tattgaaatt
     4081 gtagatatac aagagttaga atctggaata acttttaaat atgatgaaat cttaagtgta
     4141 cattcaaaag aacctacact agaagaagtt tttattaaat acacaggaag gatgttagtg
     4201 taatgtattc taaatttttt accttagtaa aaaaagattt ttggatgatg atttatggta
     4261 aattttttgt gctgacactt ggctctctca tactttattc gtgttatatt aattttggtt
     4321 atgtaaagtt tatggatgaa gatagttatc acaataatgt atatctatat aatccagaga
     4381 atataagcat gagtgcatct ccattaatta atcaagtttc ttctataggt gaattaaaag
     4441 caaaacttga tgaagataca agtgcagttt gtattgatat gagttccagt aagccgaaag
     4501 taattttaca tgaaagtatt aaaggagttg acaattatag agctgactat gcattgtcat
     4561 tattatcatc agaaaaagac cataatagca aaatatcaga caacaatgca ataattcaaa
     4621 ttaatgataa ggatacatct gagataaaac aaagaaaaga catgacttgt gagattttat
     4681 tctttgaaat tgtagctatt ggatttttag ggattgcctc tttattattt aaagaaaagc
     4741 agatgggagt aatgcgtatt actggtattt tacctgtaaa aagaagtctt tttatattat
     4801 caaagatatt tgcttttttt atatgtgacc tatgttttac agttctttta gtagtattta
     4861 atattggatt tttagaagga ataaaaatac ttccacaagt attagttcaa gctagtattt
     4921 tatcaattat tatgtctttg attggaatag gatgctcaat gttattgaga gattttaagc
     4981 aattttctct tatatatctt gtggttgctg tattcataac tacacctata tttctatctg
     5041 caaatacatc ttttgaaata gattggatta aatatcatcc cttctatcat gtttatagtc
     5101 agcttaaaaa tgcgtttttc atgaattttt caaaagaatt agtttatttt gtagtgtgtt
     5161 ctttgacaat aatagttcta tttttacttg taaaatatat ttttaataaa gaattggtta
     5221 gggagggata atcttgatag gattacgtta ccagttaaag agtataaaaa aagataaaat
     5281 gtgtataatt tccttctttt taccagttgt tatggcgatt ttaataaatt ttatgggaac
     5341 aattgatata tcttcattag gagagatacg ttttggtata gtgaaggata cacttcctgt
     5401 acaaacagag cagtggttaa agaagtatgg tatggtaagt gtatatgaca caaaaatgca
     5461 tttaatacgt gaaattaagg atattaaaac ggacacaatt ggagttgtta gagatggtaa
     5521 aggaataaaa actgtgtttt atggaaatga gattaatgct ataaaaaatg ttggaaagac
     5581 attgccagag atgtttagac taaagggtga tttaaaagat gttgaggtta caatattgcc
     5641 taaagatagt attttaaaag agtttcaaaa tgtttttatt gctatgattt taattactgc
     5701 tatgtttatg ggatgcacct ttaatgcaat gaatattata tcggaaaaag aagatggaat
     5761 atcatttata aatgaagttt tgccacagag tagaagtcag tatattatac aaaaagtatt
     5821 tttgggtctt ttatgtgggt gtttatcttc aatgataact gtatttatat gtttggatat
     5881 ttcaatatta gatatgataa tgatgttgtt actcgttgta ttttcagcat ttatttcatc
     5941 acttataggt ttatttattg gaaagttgtc agaaggtctt atggtaggaa ttgtttatat
     6001 taaaatagta atgattttat ttataggtac tcctttgtta atatatcttc taggggttaa
     6061 taccagtggt atagggaatt tatgctatat aatgccttca gttgctacat ttaatggtat
     6121 tatggagatt attaatggta atgtcattat tatcaaagat attgtaattt tggctgtaca
     6181 ttgtgtagta tggtttatga tatatattat tttagataaa aaaagaaagt tatcttttag
     6241 tagataactc agtataatta taattgggac aatttaatag gtgatttaat atactttgta
     6301 tagtgtaatt tatgtagtgc aatttgtata ctttaattta tgtagtgtaa tagttagttt
     6361 tattttataa ttattcaata taatttagta agaagttcag tacaattaga gctacttagt
     6421 ggaaaactta ataggtactt aaactatata aatactttat tttcctccat ttgtactata
     6481 aatttaattt atgatataaa tggaggtatt attatatgat ttgataaatt tcatcattat
     6541 aacctacaag taaaggtatt attatataaa gttaataaag atagtatcaa aatattattt
     6601 gctataaaga gagggactca aaataaagat aaacctattt ttcaatgttg aaatgataaa
     6661 atatatttga tttcatataa aaaattttct tcataaatta aaaagtccat tttaagcccc
     6721 ttcattttta accagataaa tcgagttaaa ctatttaaat atattactcg gattcatttg
     6781 acttagatat tccttgcttg taaaattgtt ttgaataggg gatagccaaa gcaaaaaata
     6841 aaagtataat ccctgctatg attattaaat tattaataga aattacatct gccattggtc
     6901 cgaaaatcat cataccaaga ggaataaaac cagtatatat aataataggt aagctaagaa
     6961 ctctacctag catttgtggt gctacatgct cttgcaacat agtcatagct gatgcttgaa
     7021 tgattggaat tgaaaaattc gtaaaaaaca taaatataaa aaattgccaa gcttgtgtta
     7081 caagacctag aataattgaa aaaatagcat aggataatat tgctagaaaa aaggtcttat
     7141 ttctattttt aaaaccacca aaaactccta taatcaatcc acctaaaacc atacctaaaa
     7201 atccaatcat ttcaataata gacaggtata tataaccctt gccaaatgtt cgttcaataa
     7261 gaagtcctat aagaaatcct gatggaacac aaagaagcat gaatgctcca taagtaaaaa
     7321 gcacttttct aatgaactta tctgaaaaac agtactttat ccctgatacc atatctgcaa
     7381 aaaatgagac tttttcagtt tgttttggta tatcatgttt aggaattttg atgaatatta
     7441 ataatacaat agcaataatt gcagtaaata catcaattaa aagaacttga tttattttat
     7501 taccaaatcc aagtattgca gcagctacaa taggaactat aaattgtact atagactgta
     7561 gactactatt tataccatta aaacgcatta aatgttcttc tggtacaagt tgaggaatca
     7621 ttgcgtttac agcaggtgtt tgtacaccac ttcctagtga acgtataaca gatattactg
     7681 atatggctat taattcactt gttgtgccat ttgatgacat cataaataga attagaacta
     7741 atgtagatat agcaattgtt gaatctgata caattatgag tacctttcga ttgtagtagt
     7801 ctgcccatac accagcaaaa aaagaaatta tcatttgagg tataaaggag catacagtta
     7861 aaactgttac ccatacacct gatgatgtct gaagtgtgac ataccatatc attgccattt
     7921 gtacaagtga tgagccaaat aaggaaatat attgacttgc taaaaaaaga atgacttttt
     7981 tcttccatgt agaattagta tttttatttt ccatcctatt cctcctttga tttgtgtgtt
     8041 gaatttttct tgctcttaag gataacacac aaatcaaagt ctggagttag agtggagggc
     8101 taggaaaatt gttttttcat aatttgaaga tgagatttca tggtttttgc attatgttca
     8161 gcttctttca atgaagttat cagtttgtca cacactgaga taatttcgtc atcatctttt
     8221 ggagtgtcaa ggacagtttt aatattagta ttaggactag aagataaagc atttaacatt
     8281 cgtaaaatag ctgaaagaga atagtttgca caacgaagtg cgcttataat tttaagtcgt
     8341 tttatatctt catcagtata tacacgataa ccattttgtt ttctttttac ttttaacaaa
     8401 ccattcattt cccaatttct taaagtatct attgtaacgt ttaagtgttc tgcagtttct
     8461 tttctagtta aaaagagttt atttgtttct ggagtatttc catttaaaat ctgtgcaaca
     8521 atagtaagtg cttcctcggc attagatttt tctatttcta attgttgcaa gtattgattt
     8581 acaagactaa tagccttatc aaagtcccta ttcgctgatg ttcttacaat ctcaagggca
     8641 tcttttctta atcccctttg taccacttca attttcaagg ctgtacgagc aaatctaaat
     8701 tgttctatat gaaaatcatt aaagatacga taaccattgg tattacgctt aggtttaggt
     8761 attaagttta gttcttcata taaacgtact gtattaggat gtacaccaat taaatctgct
     8821 acctctgatg tcgtgtaagt gtccattagt attcaactcc ttttagatat atagtattag
     8881 tcataataac accaaacaaa agtctggtgt tataatggag ggtttggcta tctaagagta
     8941 attagtttat gaaagagtaa ttagtttatg aaagagtaat tagtttatga aagagtaatt
     9001 aatctattat agaagtaagt atatattttt caatgaactc ccatctatca cgaggtttaa
     9061 acactatttt agatggtatg gctattacta agtcatattg tggaatacaa caaatagcat
     9121 ttcctccagt tcccattgct gaataccaca aaacaccatc ttttttacat agccaccaca
     9181 gataaccgta ttcattacta ttcattttaa ctgattcttc aatccacgat ttagatataa
     9241 tacgtttatt attccaaata ccatcattta aatacagaaa accaaatcgc aacatatctc
     9301 tagcactcaa tgtaagtccc cagccaccta tagaataacc ctgtctatct tttgcccaac
     9361 ccttaagatt ttttccaaag aaatcttcaa aaccaaaatc tttcatatca taatctggta
     9421 taactttcat accaataggt ttaaataagt actcattagc aaattcacga gcagattttc
     9481 cagtagtttt tgttataata gctgaaagta ggtgcgttcc agctgaagaa tatttgaaag
     9541 taccaatttc gcctttttga ccaagttgat tcaaggtata ttttatccaa tctttttgtt
     9601 tgcaaagtct ttctaaagac tcagtcatat ttttaaaagg gtatggtgca gtcattgtaa
     9661 gtaggtgacg tatagttact gcttttttac atatttcaga aggactacaa gtatattcag
     9721 gaaaaaaatc aacaaccttt tggtcaacat cttttatata tcctttatct attgcaatac
     9781 ctattaaggc tgatataata ctttttgtaa ctgatgcaac atgaaaagta tcatcagcat
     9841 tatatccatt atagtacttt tcaaagacaa tttttccttt tcttgcaata attataccat
     9901 ttatattgct atattgtgag attattaaag gttcaagttg tgaaagcttt tctgaactca
     9961 taccaacact tgaagggtct actttttcaa aaattaagtc taagcaattg tttttattca
    10021 ttaaagatac ctccctgtta tagattttgg attttctttt agagtttttg gagaaccttc
    10081 tgcaattata taacctcctt tgtcaccacc ttctggacca agctcaacaa tccagtcaca
    10141 aacatttaat acatctatat catgttcaac aacaattaca gaatttccat tttcaaccaa
    10201 ttcatctaat aattgaatca acttagaagt atcatataga cttagaccag tagttggctc
    10261 atccaataca taaaggatat taccatttcg ttttttacca atttcttttg caagtttgat
    10321 tctttgggat tcaccaccac ttaaagtcgg tgtaggttgg cctaatttta tataccccat
    10381 acctatttgt tctaatacat ttaagtgtga aacaatattt ggaatatctt tgaagaaatg
    10441 tacagattca gaaacactca tatttaaaat atcatgtata tttttatttt tatatttaat
    10501 agataaagat tcatcattaa atctttttcc ctgacattca ctgcatatat ggtctatact
    10561 tagattattt cccaaccaga ccttttcata tccactacca ctacatttag gacaagcccc
    10621 tttggagtta aatgagaaat gaccagctgt aaatccttgt tctaacgatt ctggctgacc
    10681 tgcaaatagt gtacgtatct tatcccatat accaatataa gaagcaggag ttgagttcat
    10741 atttttgcct ataggagatt gagatatctc tgaatatcca gaaatatgtt tagtaccaat
    10801 cagtctatct gcaatagttt caacaactgc ataatcgtct tcctcaccta tttcactctc
    10861 tgaagaattt atttgattat tgatagtatg gttattaaat tggcttctaa gaagaggaag
    10921 tagggtgtct gatataagtg aactcttgcc actacctgat tttccagata ttccaaccat
    10981 agcacccaaa ggtaatgata cagttacatt tttaagatta tttgtatttg cattttgaat
    11041 agttatacaa ggtattttat cagtatgtgt aatgttttta ttgcttgttc tattaggcat
    11101 tggatatttt ccagatagat actgtccagt aagtgatttg tcggattgaa gtagaccttc
    11161 taaatctcct tgatatacta attgaccacc ttcaacacca gcttttggac caatatcaat
    11221 aatatgttct gccatttcaa ttgtgttttt atcatgctca actactatga ccgtattgcc
    11281 taaatcttta agtttcttta gtgattttaa gatgtctatt ttttctgatt catgcaatcc
    11341 agctgttggt tcatcaagca cataaattaa tgagtccatc tctgaatcga gatgtgaatt
    11401 taaaaatagt ctttgcattt ccccaccact caaagttgac atttctcggt ataaggatag
    11461 atgacctaat cgtgattcta ttaaatgttg agttttggat aaaatatcat ttacaagatt
    11521 tgtaccaaat gtgtttaaat taccttgatt tattaagagt ttaaggaaat catcaatttc
    11581 taaaatcgtc atttttccaa gttctccaat atgttttccg tttaaaagta ctctacgtgc
    11641 ttcttcacca attttaaaac cattgcaatc tgaacaggca gtcatagtat aaactccatt
    11701 taaatcttcg ccccttttgt aacgattgtg aaataccttt gataaagagg aacttttttt
    11761 accattttca taatgaccat agagtacttc atttcttatt tcttcaggta gttgtaagaa
    11821 ttgtatgtct aaataatcat tgaatttttt agaaaatact ctcaaatatc caggtgtaat
    11881 ctttgctcta tctaagactt gttttagggt atcatgctta tcaaggacta atttttcaat
    11941 attaatctca aaatatgcac catgtccaga gcacttcata cacattccat ctgaagagtt
    12001 gtatgagaaa taacttggtc ttaatcgttc ttcctcatat ccacaattat tgcaaattag
    12061 attatcgctt acaggagtat cacacatagt acacattatt tgaccttcca ctgaatacaa
    12121 tagtgtaagc atgttgagaa tattggttct tgtgcctact gttgaacgag gattgctctg
    12181 gcgaataata ttttgttgaa cagctatagt gggagctaaa ccttgaatat tatcaaactt
    12241 atcttcacta tcaatacctg caagtatacc taaagattgc agatattgtt tacgcccttc
    12301 ctcaaataca atgtcaaaca taaggctaga cttacctgag ccactgacac ctgttgcaac
    12361 aacaagctta tcttttggaa tagaaacatc tatattttta agattgtgta tctttgctcc
    12421 attaattttt atttctttca ttggttcacc tcattttatc aattttatca aaccgtaaaa
    12481 tagggatata ttaatcctta atacaagaat agattaggtg aggttattaa aaaggttcta
    12541 taggtaaata tatatcgacc aagtgtctat tttgagggtg attgtgtggg ttgttaagat
    12601 atacttcaaa agggaaggag gcacgtagct tataattacc atttgatagc cattcattat
    12661 acataaaatc ccacgcatca ctatattcag attttgaaat atcaaaatgt cccacaagat
    12721 attttccaga aggtattatc atattgccaa gattgtcatt ctctttgaca acaatgttat
    12781 ctggaatggt catgcaaata ctagtcctta attgatgctc ctgtgtaaat tgtggattat
    12841 catgataaat ggctaatatt tgtgtgtttt caagtccaag tagattctgc tcacttgcat
    12901 atgtgaataa ccgttctaaa agttttggga aagtactggt tagattacca tatgaaccag
    12961 tatatctagt gtaaatagta ttcatattat caagttctag aatttcaatt tcacctttaa
    13021 cctctctagt attcttacat ttaggttttg atatgcttct attgtactga gaaattttat
    13081 atggttcttt gcaattcttg ctataattgt ttctatattt tatagggctt attttatagt
    13141 aatttttaaa tgctctcgag aaaactgctg aatcagtaaa gccgaagtag tgtgcaatat
    13201 cagtaacagt catgtcagtt ctatgaataa gaaaagcagt tgcattttct agttttagcc
    13261 tatttacata ttgtgccaaa gtttcttttt ctattgcttt gaagattcta tgaaaatgaa
    13321 attttgaaaa acctgctata ttagaaagtt catcaagaga aagaggttca tgtaaatgtt
    13381 cttctatata gtcttgcact ttgcaaatac gatttagata ttctttttta cttttagcat
    13441 ttgtcatata tagccctcct tattgtgtaa aatgtgtatc aattaattta tagcaatata
    13501 gtaataaaat tttatgtttt tgtaatttat gataaataag atagcgttta attttttata
    13561 attgataaaa gatataagat gccttatatt tgtctttgca tataagtata gtcttcgtaa
    13621 taattagtat accatagtga tttattttta attgtgtatt tatataaata cacaacttat
    13681 gatatggacg aattattatg caaataagct gtaattaaat aaattgcaat ataaaccagt
    13741 tgaaacataa gttagggaaa tattatcaaa gttattactt agcataagag tatagatgga
    13801 ataaaataag attaaaaaat tacatactaa atttaataaa atttttatat taaaaataat
    13861 attaagttta ttgtttaaat aaaaaagtta aatctacact attaacttat tatacttaaa
    13921 actttaaaat gaaaatatca aagaatactt tttaataaaa ataatcattt aataagttat
    13981 ttaaaaatct aaaaacttta caacaaatta tatatttaaa ttggaggagt gggaatatga
    14041 gtaatagttt ttatcaatta aattcagcag tattatattt taatgaaaaa agagatgtaa
    14101 atagaataag gtttaatcac tatgtaatga gaaaagaaaa tatacaaaga ttaaaaggaa
    14161 ctctgccaag aggacaattt atgatgacag taaggaaagc tgcatctgac cttgatgtat
    14221 ctatatctac aataagtaga cttgtaaatg aatttataga tttaggtata ttgagattaa
    14281 taagtagggg agtaaaggga aattgttgtt ctgtatatag ttatgtttgt tctgaaagta
    14341 gtaatgcaga aattgtaaca aagaatggtg atattttcaa aaatatagag aaatacattg
    14401 atgcaactat taacaataac caaaaaacaa tgaaatataa acatagaaat gagttgttta
    14461 agaacagtaa taccaacata tacagccgta taaatcaaga gagtatagac aaagattatg
    14521 atttgataat tcatactggg tatgaaacag aaaatgagac aaagaaaaaa gaattattaa
    14581 aaaataattt aaataaaaag aatttaaata aaaatacctt tgaaaattcg gatgaagatt
    14641 tgactgaaga ttctacttac aaaaatccag aaagcataca tgaaattatt ataagaaaat
    14701 taaatagtga atcaggaaaa aattttaaag ctgatttgag tataactaag gcacttatag
    14761 ataaaagatt aaaagaaggg tatagtttag aagattttta caaggttata gaggcaaagg
    14821 taagaagatg gagatataca gatatggaaa tatatataga accaacaatt ctgtttgggt
    14881 atgaatttga gtcgtattta aatgaggaga gttctttatc tgcttgtaat tatggtgata
    14941 tgtgttatga agatatgtat tgagatattt attaagatat tagttgcttt ttagttgttt
    15001 aaaatatgtt tatgtataaa tagtgtatta aaatggagtt gtctcaaaat agagagaagt
    15061 ctcttcttaa ttgtttaaat gatgaaaata tatttgattt aatacaaagg agagtgcttt
    15121 atgagttaga agatttattt tgagacactc acttttttta atatactgga gaaatataac
    15181 ccattttaca agagtgcata gatatatata atggcataat gtggatttaa attaaaaaaa
    15241 taaaataaag ccataaatat tgaaaatttc aatatttatg gctttattgc ttgtaataat
    15301 taatagaata tatatttttt attaatcatc tatttatatt ttttattaat catctatttc
    15361 aaattctaat attttaccag taattgcatt aattgttatc tcatattcag tttttccttt
    15421 tattatttca ccttcatatt ctttgtcatc ttcatcatac ttaaatttta ctattttccc
    15481 accaggcact ttatctagca ttattgattt agctttttca gcactaataa tagaactgtt
    15541 gtcattagta ttagagttat tcttatcatt atcatcaaag tcatcatcag aatcattgtc
    15601 catgtcaata tctacaattc ttccatcaaa tgcacttatc tctaattcat atgttttctt
    15661 attttttatt actttagcct catattcgtt atcttcagca tcatatttac aatagattaa
    15721 attaccatta ggaatagcat ctaatgctat agacttagct ttatccaatg atatattttg
    15781 tgtacctaaa ttattatcat ttaagtcatc atctttttca atttctatta ttttaccaga
    15841 gtaggcatca atttctatct catataggat attatccttt ataactttgc agtcatattc
    15901 attatcatct tcatcataat aacaaactaa aatattgctt cctggaagag cttgttttaa
    15961 tatttcttta gcctgtatta aagatatttt tttagtttta tttcttatgt tcacattttt
    16021 tagttttaaa atatcttcaa atgctttttg attatatcct cctcctactt gtgttatttc
    16081 atctatatct ttatttttaa taaattcttt ttgactattt acaaggctac cattagatat
    16141 cattatagga gaattttctt tagcagctag tactcctatt gataatgaat ctactaatgc
    16201 attttcttgg ttaataccat ctttagctac aaatacatta tttatttttt tatctttata
    16261 aaatttatca ataatttttg cattagtatt gtttctatct gaaccactga ttctttctct
    16321 gtttggatat ttctttactg tattgtctga taaaatctta tttcctccaa ctatgtaaga
    16381 tttttttata gacctattgt ttaagaaatt tatactacta tcattgctgt tttcatctga
    16441 tactaatatt ataggcatat tatgtattgc tgctggagaa gctatactaa ctgcatcaga
    16501 tagtcccttt agtccattta caactgcaac attttctatc ttttttatct tatctatttc
    16561 tttaagtaca tttgtagaca tttcatatat attgtttcct actattttat ttatttttat
    16621 gttaagtgat tttatttcat tttctacttt agaagaaatg ctatttccac tatctacaat
    16681 gtacacatta tctacaccaa gccttttaag ctcagcctta gttgaatttt caatactgtt
    16741 gttgtttact aaaagtatag gtgcattctt tagcttagca aatggatttg cacaaagcgc
    16801 actttgaata gaattaccat taactataat ggctgttttt gattgaaccc atcccaattt
    16861 acttacttct actgctgttt catatcttgt tttacctatt aatctttctt gctttgtagt
    16921 attgtctgca aaaattgctg aagaactaga aaaaaacatc atacctgata ttaatatagc
    16981 taaatttttc ttattcatat ttttacctca caatctttaa aaatttgtta tttagcatac
    17041 ttaataaaat ataatcttaa aatcaaattg atactgagtt tgaagttaca aaatcaacaa
    17101 ataagatttc tttaatacaa aattttcctt tttatattta acattgtatc acaaatataa
    17161 aaaggaaaaa actttttaaa aagggtatac actattcatt ttttatctta taaataagtc
    17221 tttatcactg ttttatatat tttaaaatag acacaagacc tatttctaaa tcttttataa
    17281 atattttagg tgaaatttct ttttaaacat atctaaaaag tatttgtagt aatattaatg
    17341 gactgaattt cttaaagtat atatttcttt acatgttgga gtttgtgaag tttgagaatt
    17401 ggagggtgaa agaaacatgt aatggattaa tattaaagaa ctgtattttt ataatatttg
    17461 tcttaaaaat aaaaaaatct attgacttga agttaactcc aagtagtaaa gtataattat
    17521 acaagaatta tatacttgta agaatagtta aagtagagag aaaaaacaaa caagttttaa
    17581 agggggaagt aaaacaatgg aggcaaaaaa attaggattt ggattaatgc gtttaccaat
    17641 aaaagatgaa aatgatgtta caactattga tatggaacat ttaaataaaa tggtagacac
    17701 ttttttagaa aggggattta catattttga tacggcctat gtgtatcaca tgggaaaaag
    17761 tgagatagca cttagagaat ctctagtaaa aagacacaaa agagagagtt ttactatagc
    17821 tacaaaactt ccattaatgg ctttaaagaa aaaagaagag caagaaacca tttttaatga
    17881 gcaattagaa aaatgtggtg tagactattt tgactactat cttttacaca atataggcgt
    17941 gtcacattat gaagtagcta aaaaatttga tagctttaag tttattgaag aaaagaaaaa
    18001 agaaggaaaa ataaagaata ttggtttttc ttttcatgat agtgcagaat tattggataa
    18061 agtcttgagt gagcatccag aagtagactt cgtgcaatta cagattaatt atttagattg
    18121 ggataatgaa agcatacaat ctagaaaatg ctacgaggtg gcagagaagc ataataagcc
    18181 tgtaattgtt atggagccag taaaaggagg tacacttgca aagataccag aaaaagcaga
    18241 gaaattattg aatgactaca atccagatat gtcaatttca tcttgggcaa tacgttttgc
    18301 agcaagtaaa gataatgtta tgatggtctt aagtgggatg tcaaatatgg aacaattatt
    18361 agacaataca ggatatatgc aaaatttcaa accattcgta caagaggaat ataatattat
    18421 agatgaagca gtagagatta tcaacgaatc aattttaatt ccttgtactg cctgtcagta
    18481 ttgtgtagaa ggctgtccaa aaaatatcgc tattccaaac tattttgcgt tgtacaatgc
    18541 agaaaaacaa gctcttaata ctggattttc aacacagatg gtctactata ataattatac
    18601 taaaacttat ggaaaggcct cagattgtat tgaatgtaaa caatgtgaag aaagctgtcc
    18661 tcaacacatt aaaattatag atgctttaaa gaatgtagca gaaacctttg agaaataatt
    18721 caatctatac aatatcaaga ggagaagata tgtatgacga tttcagaagt tagtaaaaaa
    18781 tacgagcttt cagcggatac tttacgatat tatgaacgaa ttggtttaat accacctgta
    18841 aatagaaata aaagtggaat taggtctttt acagaagaag attgtgagtg ggttaatttc
    18901 ataaagtgta tgagaggtgc aggtctttct attgagacat taattgagta tgtagctatg
    18961 tttcaacaag ggagcagtac tatcaaagct agaaaagaac ttttaataga gcagagaaac
    19021 caactagcaa aaagaataga agaaatgcaa aaaactttgg aacgattaaa ttttaagatt
    19081 gatagatatg aagaaggtat tattgagaaa gaaaaagtat taaagagcag tagaaataag
    19141 aaaatggagg cagtatctta tgagtaaaaa tgtattaata gtatcagcta gtcctagaaa
    19201 aggtggaaat tctgatttat tgtgtgacca gtttatacaa ggggtattag aatctaaaaa
    19261 tcaagtggag aagatatttt taaaagataa agatattaac tattgtactg gatgtggtgc
    19321 ttgttatgaa aaagaagcaa attgttcaca gacagatgac atgggagaaa ttcttgaaaa
    19381 aatgattggg gcagatgtga ttgttttagc gactccagtt tatttttata ctatgaatgc
    19441 gcaaatgaaa actatgattg atagaacttg ctctagatat actgaaattt gtgataaaga
    19501 tttctatttt atcgttacag cagcagatga aaatgtggct gatatggaaa gaacaataga
    19561 aggatttaga ggatttacat catgtctaaa tggttctaag gaaaaaggta tagtatatgg
    19621 tgtaggtgct tggaatgtag gtgatattaa gggaagtaag gcaatgaaac aggcatatga
    19681 aatgggtaag ttggcgtagt atgtttgtat aaaattgtat aaaattgaat atattcttat
    19741 catttcaaca ataaaaagag gcttatctgt attttgagac agcctctttg tgctatatca
    19801 tttattttta ttctatatcc ttattatacc ctttattttt ttatgcctat ttaagtatct
    19861 gttgttactc atatatttta ataattaccc ttaaaaaact atacagattc ctgcataata
    19921 ttattttctt tagtaattaa tttttctagg tttttccaat ccatttcttc catatttgca
    19981 aaataatgac cacttcctat gcaagtcagg taagactttc catctaattc aaacgaagaa
    20041 cctccaatac actttgaatt acatttctta caagtacgag ttttagttat actatcttta
    20101 atagttttag agcattcctc cacagcatct ttatatgtat ttagatgaaa aagatttgct
    20161 ttaactctcc attttttttc attacattca atagtaaata agctacgttt tggtttctta
    20221 gttgaatata ctattttgta agaacgagca cctgtttttg gttcaatttt acaatctaat
    20281 gatttaccaa gttcatgtaa atgacatgca atatcataat ctttaccact tagtacttct
    20341 aacatttctt ctgttgtttc tgctgttaat tcacttttgc tcatattaaa aaaatccccc
    20401 tatataaata ataatttcat atgaaaataa ttaatataag tatattaaat aaaactatta
    20461 aaatcaataa aattactgca atcttacatt attgggcata gaatgagagc taatgattta
    20521 ttagaaataa gacttaatag tatgaaaatt tacttagacg tggtacaatt ctataaaaga
    20581 tttaaaacta ggaaaggaga aagttgttga gatacggcca atataacaat ttatttataa
    20641 gttgtaaatt tattatgaga ttaattagat tgttaagtaa tactatcaaa aggtagtttt
    20701 ttggattttc agcaacagtg gtagatattg ataatttttt ggttattatt atatttagct
    20761 gttatatttt caataggctc tatgataact agggtaaata aggtatgggt aaaggtttta
    20821 ttatttataa tagtgggatt gtcaactata gttctttcat atgtaataac ttgattatgc
    20881 aaaattctat gaagcaggaa ccctgtgact ttagtcatgg gaggttcaga atttaaggtt
    20941 aaaaaataac tttaatctgt aaattctcaa aacaagaatt ctcatgttgg taaatttcta
    21001 gatatgagaa aaaaattgac atcataaaaa agtaatttta gagcgttttt ttacgaaaaa
    21061 attcagaagt aactattttg aatgacttca caaaatttta tatataaata aagtttggca
    21121 cgttaattgc tttaataaaa gttaacaaga cacaaataaa cataatcatc taagtattga
    21181 ttatttgaag ttttagattt gaagtttaat ctaaaggttc gtattaatat tggtgtttag
    21241 gaaattttat tttcctgtaa atataactta ataataggag gtgaagtcat gtcaaaagta
    21301 gtaatagcta cagagaaagc tccaggagca attggaccat attctcaagc aattcaagtt
    21361 ggaaacatag tttctacatc aggtcaactt ccaatagata tgaagactgg cgagttagta
    21421 tctgacccaa aagaagcaac aaaagcttct ttagataatg taaaagcaat attagaacaa
    21481 gctggtacaa gtatggataa aatatttaag actacagtat tcgtaaaaga tttaaaggat
    21541 tttaatgata ttaatgaagt atatgctaca tattttaaag aaaatccacc agcaaggtca
    21601 tgtgtacaag ttgctcaatt accaaaagat gcagttttag aaattgaagc aatggcagta
    21661 ttataagtat tataacaata aaaccaataa caaaactaat aaaaattaaa aactaataaa
    21721 aaaagtctac aattaaaaaa gtttatatta aaaaagtcta tataaaaatc tatctaaata
    21781 tttaactatt gagcttttct catataatgt atatttttat gtcaaaaata tatttgttat
    21841 atgaggaaag ctttttttta ttattgtatt gtgatttatt ttattgtctg catattataa
    21901 ttaaggttac tatataaaac aaacttttta taaattaata ttgtctgttt taacgcaaat
    21961 atataaataa gtaatgtttt atatatattt taaagagtga ctatatgtta aaatatagct
    22021 aaactgataa tttggtaaat taaattctaa tgggggaaac tatgttaagt aaattaaaag
    22081 aatttcaaca agaaatgata aaatacactg aaacagtagc tagtgtgtta gatgtagata
    22141 ttgaaatagt tgacgataga ttaataagaa ttagtggtac aggtctgtat aaatcaaaga
    22201 taaatgaaag cgttgttact gagggcttta tttatgacaa tgttatacag acaggtcagg
    22261 aattagtcgt attggatata tgtgataatc agctttgtat agaatgttcc cattacatga
    22321 aatgcctaaa taaagtaata atagcagttc caataaagta taataatcga actataggag
    22381 taattggtgc tattagcaca gataaaacta aaaaagtaga aataagtgca aaaatagata
    22441 attatttaaa atttgtcaat catatatgcg atttaatttc tatgaaaata gaagaacatg
    22501 aagcgtcaaa aaatagcagt agaaaaatgg atatgatgat agaaatcatt gaaaatgttg
    22561 aaaaaggcgt tataatacta gacataaata gcaaaatatc ttatataaac aatatagctc
    22621 taaaaaaatt agatatagat aaaaatataa tagaaaatat agtaaatata gtatctgttg
    22681 aatcttcctc aaatggacat gaattactag aaattgatat tgataacaaa atttataata
    22741 taaatgccaa aataattccg gtatatccat atataaatca atacgacaaa ataataatat
    22801 ttgacaagac atatatcaat cataaaggac atggaaaagt gaatagtggc tggggaaata
    22861 gtgatataga gtctataatt ggaaattcag aagcaatgtt aaaagtaaaa gagagaacta
    22921 agaaattagc aaagtcaaat tcaactgtac ttataactgg ggaaagtggt acgggtaaag
    22981 agctaattgc tagggctata catgctgaag gtagcagatg gaacaagcca tttattgcga
    23041 taaactgtgc tgctattcca gaaaatctcc ttgaaagtga gctatttggc tatataaaag
    23101 gtgcatttag tggagctagt agtggaggga aagtaggtaa atttgaactt gcaaatgaag
    23161 gtgttatatt tcttgatgaa ataggagatt tgtcaatgcc tttacaagca aagttactta
    23221 gagttttaca agagagaaaa tttgcgagaa ttgggtcaaa taaacttata gatttagata
    23281 taagagtgat tgctgctaca aataaaaact tattgaaatt agtaaatgaa ggtaagttta
    23341 gggatgattt atactataga ttgaatgtaa ttcctataaa tttgccacct cttagggaaa
    23401 gaaaggatga tattgaggca ataatgatga agtttgcttc taaatattct ttagaattag
    23461 gtattcagtt aaataaaata gaagaaaatg taatgaatat gttgattaac tataattggc
    23521 cgggaaatat aagggaactg gaaaatgcag ttgaatatat gatgaatttg gttggagatg
    23581 atggaataat atataaagat atgcttccat tagatatttt aaactattat aatattaatg
    23641 gaaatattta taaaaataag gatgtaaata taatatttga agatgatata gttggaggga
    23701 tagtagagaa tcaagaaagg atactttcaa ttaaagaatt agagttgact tacataaaca
    23761 agttgttgaa taagtatgga agagatacaa agacgaagaa gaagatagca aaagacttag
    23821 gcataggttt ggctacacta tatagaaaat tggaagagga acagtagtaa tataatatta
    23881 aaatgatagg tatttgtgtg tttttataga taggtatttt tactttatgg tggaggtgtt
    23941 attatgcaat atgcgataga gctatattat gataaaaaaa cggaaaagca actttttgat
    24001 ttatctaaaa aaatcgctga tgaaaaaatt agtacgaagt acttagaatg gaaaacaaga
    24061 cctcatctta ctttggcttg tttcaatgat gtggatgagg cttgttgcat agacaagcta
    24121 aaggggtttg cacaaaacca taaggtaata cctgcttaca ttggttcagt aggcatgttt
    24181 aatgatacta aaacggtgtt tgtatcacct ataatgaata gcaatgtatc agtttcagag
    24241 agaattatat gaggtactac aagattttga ttcaaatggt tgggaatggt accaccccaa
    24301 tcgttgggta ccccattgca caatagcact tactggagat gatgaaagtg aggctttttt
    24361 tcgggcaagt gatgtattac tgcgtgagtt caaaaaaata agtggtgagt ttgtctcaat
    24421 aggattggtt aaaattacat ttcctgtgga agaaattttt acagtgaata tgaataggta
    24481 actttaactc tgttgtggat gttatgaata ttttgtatag tgcatttcat cagataaatg
    24541 cgaagttgcg aatttcgaca gctcaatagt cgcaagtagg gcacaatggt acacatattg
    24601 cacttttgct tattgggggg acgaccatgt aaacttctgt aagaagctat gaaaagattt
    24661 aagccatttt ggaggctttg ggtaataaag aatatttggg taataaagta tataaattta
    24721 tagagtttat gcgtaagaag gttatagcca tatatttata gaaaataaaa gtagtaatat
    24781 atctgacaaa acaatagaca tataaaaaat aagagcataa taaaggaaga attaagataa
    24841 ataaacgatt tctcgaattg ataattctcg tattgataaa aatttcccaa tatgagaatt
    24901 ttttctttta taaatcttat ttttcacact caaaatttat aaactttaaa gaaattttat
    24961 aaaagtatgt aattccaaca taaaaaaatt gtttttagaa taaataatat tggcacggta
    25021 attgctttgc tataaggcac aaagataaaa aattaaaatg acaccgaaaa atttgaaagg
    25081 ggaatataaa ataaatgaga gaaataaaat ggaaattaaa taacttacct aaatcagagg
    25141 aaaaagaaaa aggaattgaa tttttaaaag atgaagagat tgcaaaggct aaagcttttc
    25201 atgaaagctt tccacaatat gaaaagactc ctttagttaa attagataac ttagcaaaat
    25261 cactaggcgt aagcggaata tatgttaaag acgaatctta tagatttggc ttaaacgcat
    25321 ttaaagtttt aggtggttct tactctatgg gtagatactt agctcaaaga ttaaatacag
    25381 atatatctga gttagggtat gacaaattga cttcgaaaga aataaaagaa aaattaggag
    25441 aaataacttt ctttacagct acagatggaa atcacggtag aggagttgct tggacagcta
    25501 ataaattagg tcaaaaatct gttgtattaa tgccaaaagg ttcttctgaa tttagattaa
    25561 ataaaatcaa aggagaaggt gcagatgcta gtataacaga cttaaactat gatgatgcag
    25621 taagattagc taatgattat gcagaagctg atgaccatgg agtaatggtt caagatacag
    25681 catgggacgg atatgaagaa ataccagcat ggataatgca aggatatgga actatggcta
    25741 aagaagctat agaacaatta aaagaatatg gagtagatag accaacacac gtatttgttc
    25801 aagcaggagt tggttcactt gctggtgcag ttcaagggta tgttgcttca atatatgatg
    25861 aatgcccaat aacagtagta gtagaggctg atgaggctga ttgttactat aaatcagcag
    25921 aagctggaga tggaaaacca agatttgttg gaggagatat gccaactata atggcagggc
    25981 ttgcttgtgg agaaccaaac actataggtt ttgaagtgtt aaaaaattat tcatcagcat
    26041 tcgtttcagc accagactgg gtatctgcta aaggtatgag aatattagga aaccctctaa
    26101 gaggagatga gaaagtaatc tctggagaat ctggagcagt tacaactgga gcattagttt
    26161 caatccttga aagtgaagat ttaaaagact taagagaagc tttgaaacta gatgagaact
    26221 ctaaagtatt attaataagt acagaaggag atactgaccc tgataaatat agagatatag
    26281 tttggaatgg tgaatgccaa agtaaataaa agacttaagg aaaataaaaa cttaacaaat
    26341 aaatgaaaaa tttaaggaaa ataaaaactt aaataagtga aaactttaaa taaaataaaa
    26401 actaaaacaa atgaaaaatc taaataaatg aaaaagtaaa gcaaatgaaa aattcaaaat
    26461 aaaaactaaa taaaattaaa taaaaccaaa taaaattaaa ataaagtcta aataaaaatt
    26521 taataaataa aatataaaga aataaaaaat caatagatta agtgaaatgc taataaatta
    26581 aaaaagtgaa atatcaaaat aaaacgtcaa caaactaaaa attatatggg ggaatatcaa
    26641 aatgttaaat gaaacaagaa aaaaacaatt aactgaagta tgccaagact taataagaaa
    26701 tgctagttat tcaggtcaag aagaaaacgt agtaaaagct atagaagaaa attttgcttt
    26761 actaggtttt gatagttggt caagagatag atatggaaat atagtaggat gtataaaagg
    26821 aaataaacca ggtaaaaaaa tattatttga cgggcatata gatacagttc cagttccaga
    26881 tgcaagtaaa tggagtgttc ctccttttga agcgagaata gtagatggga aaatatatgg
    26941 tagaggaact tctgatatga aggggcaagt aagtgctatg atggctgctg tttcatactt
    27001 tgcagaagat actaacaaag actttgaagg tgaattgtat gtagcaggtg ttgttcatga
    27061 agaaatattt gaaggtgttt cagctagaga gataagtaag gctgttcaac ctgattatgt
    27121 tgtaataggt gaatcttcag aattaaactt aaagataggt caaagaggta gaggagaaat
    27181 agtagtagaa acttttggta aaccagctca ctctgctaat ccagaaaaag gtataaatgc
    27241 agtttataaa atggctaatg taatccaaaa gatacaacaa ttagtacctc caactcatcc
    27301 agttttaggt gatggaatat tagttttaac agatataaaa tcttctccat atccaggagc
    27361 atcagtagtg ccagattatt gcaaagctac ttttgataga agattattag ttggagaaac
    27421 aagagaaggc gtattagctc caatacaagc tttattagat gaaatgatga aagaagaccc
    27481 agaattaaat gcaaaagttt cttatgcagt tgaaaaagct gattgctata caggaaatac
    27541 tatagaaagt gaaagattct tcccaggttg gttatatgat gaagaagatg aatttgttca
    27601 agcagcgtat aagggattaa aagaagctgg aatagattca gaaataactc aatactcatt
    27661 ctgtacgaat ggaagtcact acgcaggtga ggctggaata agaactatag gatttggtcc
    27721 ttctaaggaa aatcttgctc atacaataga tgagtatata gaattagacc aattattcaa
    27781 aggtgcagaa ggatattatg gtatattaaa atcagtttat aaaaaataat taggagatgg
    27841 ttttgtgaga actttaataa aacatgggct tatagtagat ggaaataaaa caccagcata
    27901 tgaaggagat attttaatag aaaacgagaa aatattaaaa atatcacaag atttaaacga
    27961 agaagctgac aaagttatag atgcaaaagg tagagttata tgtccaggtt tcatagatac
    28021 tcatagccat tcagacctag ttattttagt taatccatat aatgaggtaa agattagaca
    28081 gggaattaca acagaagtgt tgggccaaga tggtatatct atggcccctc tacctcaaga
    28141 gcatatatct tcttggagaa aaaatttagc aggtctagat ggtgaaagtg atgaaattga
    28201 ttggaaatat gaaacaacag agaattattt aaaaatgatg gattacaatg gggtaggttt
    28261 aaatgaaact tatttagtac ctcatggaaa cgttagaatg gaagctatgg gacttgaaga
    28321 tagacctgct actaaagaag aaattcaaaa aatgtgtgaa atcacagaga gagaattaaa
    28381 agcaggagct ataggattgt ctactggact tatatatata ccttgtgcat actcattgac
    28441 tgaagaaatt atagagatgt gtaaggtagt agcaaaatat gacggagtat ttgtagttca
    28501 ccaaagaagt gaagcagata caattttaac ttcaatggaa gaaatcatag aaattggaaa
    28561 acaatcagga gtaaaagttc atttttcaca ttttaaggtt tgtggtaaag caaactggaa
    28621 gtatatacca caagttatag agcttttaga aaaagcagaa aaggaaggaa taagagtatc
    28681 ttttgaccaa tatccatatg cagcaggaag tacaatgctt ggagtagttt tacctccttg
    28741 ggcacattct ggaggaactg ataagttaat agaaagatta agtgatgaaa acgaaagagc
    28801 aaaaatgaaa aaagatatag caaatggaat agaaggctgg gataatttta tagaatttgc
    28861 tggaatagac caaatatttg taacaagcgt aaaaacagaa aagaataaag atacaattgg
    28921 taagagctta ttagaaattg gaaaaatgag aggaaaagac ccattagatg caacgtttga
    28981 tttattaaaa gaagaagaaa atgcagttgg gatggtagat ttctatggat tagaagaaca
    29041 tataatagga ttcatgaaaa gagacgagca aaatgtgtgt acagatggtc ttttagcagg
    29101 taaaccacat ccaagagctt atggttcttt ccctaaaata ctaggaagat atgttagaga
    29161 gttgaatgtg ttaacaatcg aagaagctgt ttataaaatg actaaaaagg cagctacaag
    29221 ctttagtata aaagatagag gcgaattaaa agaaggatat tttgcagata tagttatatt
    29281 tgacaaggat acagttagtg gttgtgatga ttttatcaac tctatgcagt accctacagg
    29341 tatagattac gtaattataa atggaaattg tgttgttgaa gaaggaaaat acaataaaat
    29401 taaagcagga aaagtactta aaaattaaat cttagcttta aggaggaata aatttatgat
    29461 tttagtgggg aatgggaggc taataagcca agattcttta aatccctata tggaagatgg
    29521 atgtgtagta ataaatgata atctaattga agatataggt actacagaag aaatgaaagc
    29581 taaatatcca gtacatgaat ttatagatgc aggcaagaaa ataatcatgc cgggtctaat
    29641 aaattcacat atgcatatat acagctcatt tgctagagga atggcagttc caggaaaacc
    29701 ttctgagaac tttttagaga tactaaataa tttatggtgg agacttgaca aacagcttac
    29761 actggaagat acaaaataca gtgcatatgc aacgtatata gaatgtataa aaaatggtgt
    29821 aacaacagtt tatgaccatc atgcaagtcc aaatgcaatt gaaggcagtt tgtttacaat
    29881 atcagatgtt gcaaaggatt tgggaattag aacgtgtctt tgttatgaag tgtctgatag
    29941 agatggtgga aatacaatcc aacaaggtat caatgaaaac gtaaacttta taaaatacgc
    30001 tcaaaaagat gatacagaca tgataaaagg aatgtttggg cttcatgctg catttacact
    30061 atctgatgat acattaaata aatgtgcttc agagatggct ggtcttgata caggttacca
    30121 tgtacatgtt gctgaaggac tagcagactt acaatataat gtagataaat atggaaaaag
    30181 ggttttagaa agattcaatg attttggagt actaaaagaa aaaacaatag cagttcattg
    30241 tatatatata agtgcagaag aaatcagtct tttaaataaa acaaatacat ttgtagttca
    30301 taatccagaa tctaatatgg gaaatgcagt tggatgttct cctgcaatag agttattaaa
    30361 aaggggagtt acagtaggat taggaactga tggatatact agcgatatgt ttgaatcatt
    30421 aaaagttgcc aatataattc acaaacatca cttaaaagac ccaagagttg gatttgaaga
    30481 atcacaaaca atgttatttg ataacaatag aaaaatagct aaagcttatt ataaaaatga
    30541 cctaggagta cttaaaaaag gtgcttatgc agatgttata gtagttgatt atataccaca
    30601 tactccattg acagaaaata caataggggg acacactatt tttggaatga gtggaagaag
    30661 cgtagataca actattataa atggaaaggt acttatgaaa gatagaaagt taataaacat
    30721 agatgaagaa agtattttag caaaaagtag agaattaagt caaaatctat ggaatagaat
    30781 ttaaataatt gatattaata tacaagtaag aaacaagaat ttagatgtta aaaacagagc
    30841 ttaaacaaaa ataggaattt aaatgaagta tataaataaa aaatatacgg aatagaatat
    30901 taaattaaat atagaaggga gacccaaaat gtctcgagat aaaagcccta aaatagctcc
    30961 agtagatgaa aggttacctg tatccaaatc ttgggtattt tcactacagc acgttttagc
    31021 aatgtgtgca ggagcagtag cagttcctat gatgatagga agtgctgcac gactagatca
    31081 tgctagtatt gtttttttaa taaatgcttg cttgtttatg gcaggtatag gaacactcat
    31141 acaaagtgtt ggtataggaa atattgtagg tgctaaaata ccagtaatag aaggaactag
    31201 ttttgccgct gtggcaggta tgacagcaat agcctcatca tatagtgatt cacaagttgc
    31261 aatacagaca gtatttggct ctgtaatagt agctggattg ttttgtatat tacttgctcc
    31321 agtatttgaa aaattaataa gatttttccc tagtgtggtt actggaacag ttgttatggt
    31381 aataggtatt tcactatttc cagttgggat aaaatggata actgacaaca aattggaagc
    31441 ggttgaacca aactcattag cattagcttt agcagtattg gcaataactt tactttcatt
    31501 taaattctta aaaggaattt ggaatagtac agctatattg gtaggtatag tacttggtac
    31561 actactttcc atggcagttg gaatggcaga ttttagtgca gtaaatggag ttggttggct
    31621 taacttaaat gtacctttaa aatttggaat gcctagattt gaaccatctg caatactatc
    31681 attgtgtttg attatgttgg tacttatgac ggaatcagtt ggtaatatga tagctattca
    31741 tgaaatggta gacaaagaag taacaggtaa aaatattaaa agaggtctat ttggagatgg
    31801 aatatctaca gcattatctg gaatattcaa ttcatttcca ataactccat ttggtcaaaa
    31861 tacaggaata gtaggtctta caggtataaa atcaagattt atagcagttt acgcaggggt
    31921 tatattgcta gtacttggat tcttaccaaa atttgcagca ataataggag caatacctaa
    31981 gcctgttctt ggaggcgtag gatttgcaat gtttggaatg gtaatggttg gaggagtaaa
    32041 aactttaagc aaagtgaaat ttgatggaac taaaaatggg gttatagtag cagttagtgt
    32101 tggtttgtct atgataccac taacaaatcc tgatttctat cataatttcc caacttgggt
    32161 acaaacaata ttccatagtg gaataacaac aggtagttta tcagcagtgc ttttaaacat
    32221 attcttcaat gaactaggtt ctaaaaaaga aaaaaataat aaagttgaag aagcaaaata
    32281 aaaataaaac aagtaaaaat ctatattgac ttaaagtcat taagcattaa taaaaataat
    32341 tcagagatat taataattca acaaaactat cttatatttt aaataatata aaaaagtaaa
    32401 taaataacga atacaaataa aagaatacaa tctaaatatt aaaaatattt aatttaaggg
    32461 agagataata atatggctaa tttaaaagtt aatttatatg gaatggaatt tttaaaccca
    32521 attatgacag cggcaggacc tggagcaaaa gatggtgctc tctgtcaaga ggcagcaaaa
    32581 ggtggagcag gagctatatg tacaaaaact atatctgtat taccagctga tgtaccaaga
    32641 ccatgtatgg caaaaactaa tagtggtttt ttaaacacag aattatggtc agagcttcca
    32701 aaagagcaat ggatagagga agaatacaaa aaggtaaaag aaacagggct tccagtaata
    32761 gtaagtatgg gatattcagc agagcaaata gctgaggttg ctcctttagt aaagccttat
    32821 gcagatgcag ttgaattatc tactcattat gtaggtacag acgttgcacc aatagtaaat
    32881 gctttaaaag ctgcaaaagc tgttttagat gtaccagtat ttatgaaaat gagtcctcat
    32941 actgacattc aaactattgc aaaagctgtt gaagaagcag gtgcagatgg acttgtaatg
    33001 ataaactcat ttggaccatg tatggcaata gatgtaaata caggataccc tataatggga
    33061 agtaaagctg gatacggatg gttatcaggt gcaccaataa gacctctagc aataagatgt
    33121 atatatgaag cttctcaagt tgtaaatata ccaataatcg gtgttggtgg aatcacttgt
    33181 ggaaaagacg tggctgaaat gatgatggct ggagcaagtg ctgttcaagt atgtacagaa
    33241 gcaatcctta aagggccttc tatctatgga aaaattgcta aagaattaaa tgaattttta
    33301 gatgagaatg gatatgctga tgttaatgaa ataaaaggat taactcataa gaaaatggca
    33361 gctagagggt ttagaactca ttcagtagct ccagtagtgg atgataacaa gtgtatcaaa
    33421 tgtaagcaat gtgtaacaag ctgtgtatac gaagctttag aagttacaga taagttgaac
    33481 atagatgcag acaagtgttt tggatgtgga ttatgtgtaa ctagatgtcc aaaaggagct
    33541 ttaagcatct gctacaatca agtagaagct atgaatgagt aataattata tagaattttg
    33601 gtaattaact tagagtaata ggtattagga taataaaata tatcagataa acaaacacgc
    33661 attatgcatt aataatacag tctaaagtta ataattttat tatattgatt taactaaata
    33721 ttagtttagc tgaatattaa tttaattagg tattggttta atcttaatat agatttacta
    33781 cgtataagtg aactaactag attactttaa gttaattttt tcatataaaa taaattgata
    33841 cattacaagt aaggtggagt ctatatggga attgttttaa aaggtgggaa agtagtaaca
    33901 agtgaaaaaa cttactgtgc agatgtaaga atagaagatg aaaaaatagt cgatataggt
    33961 cttgatatat caaaagaaaa tgatgaaata gttgatgtta gtgattctta tgttatacca
    34021 ggaggtatag atacacacac acactttgac ttagatacag gcgttactaa gacagcagat
    34081 aactttgaga caggaacaaa agctgctata gtaggaggaa ctactactat attggatttt
    34141 gctacacaag ctaaaggatg ttctttgaaa gacggattag aagaatggca tgaaaaagct
    34201 aaggataaat catattcaga ttatggcttt catatggcta taacagattg gaatgactcg
    34261 gtttgtaatg aaatggagga tatggtgaaa gagggagtat cttcatttaa actgtatatg
    34321 gcgtataaag gaagccttca agttgatgat ggtgtaatct ttgaagcatt gagaaaagca
    34381 gaagagatag gagggattat tggtttccat tgtgaaaatg gggacataat atgtgaactt
    34441 gtggataaag caaagtcaga aaataattta tctccaaaat atcatcaatt aactagacca
    34501 gctataatgg aggcagaagc tacatcaaga cttatgaaga tagctgaagt tgcaaactct
    34561 aaagtatatg tagttcactt aagctgtaaa gaatccttag aggaggtttt aaaagctaag
    34621 caaagaggtg tggatgtaat agttgaaact tgtcctcaat accttctatt aaatgatgaa
    34681 ttatatgatt tagatggatt tgaaagtgct aagtatgtaa tgtcaccacc tttaagaaat
    34741 aaagaaaata atgaaatatt atggaatgct attttagata atcaaataga cactatagga
    34801 acagaccatt gttcttttaa ttataaggga cagaaggaac ttgggataaa cgatttcagt
    34861 aagataccaa atggttctcc aggtgtagaa cataggatga gtcttttata cacatatgga
    34921 gtctgtaaaa ataaaatatc tatcaacaag atggtagaag taacatcaac taatgcagca
    34981 aagttgtttg gaatgtatcc taataaagga actatagaaa taggcagtga tgcagatata
    35041 ttagtcttaa atccaaacaa aaaatccaaa atagaagcaa aaaaacaatt acaaaatgta
    35101 gattatacac cttatgaagg gtatgaagtg gattgtcaaa tagaacatgt ttatttaaga
    35161 ggaaaagaag tagttagaga tggagaatta atttgtaata ctccaagtgg gagatatata
    35221 aataggcgtc taacaaaggg ggctagagaa tggtagaatt tatattaaat ggagagaaag
    35281 ttgttgcaaa agaaggcaaa aaacttttag acttccttag agaagatatg aatataactt
    35341 cagttaaaaa tggatgttca gaaggtgctt gtggaacatg tatggttata atagatggta
    35401 aagctactaa agcatgtgta tttaaaactg ataaattagc tgggaaaaac ataacaacta
    35461 tagagggatt atcagagaga gaaagagatg tttttgcata ttcttttgct agtcaaggag
    35521 ctgtacaatg tggattttgt attccaggta tggtaattag ttcaaaagca cttatagacg
    35581 ttaataataa tcctactgaa gatgatataa agaaagcttt aaaaggtaat atatgtagat
    35641 gtacagggta tgtaaaaata attaaagctg tcgatttagc agcaaagatt tttagagaaa
    35701 atatagatgt gccaaaagta aactgtaaag gtctagtagg tgaaaatctt cctagagttg
    35761 atgcagttgg caaagcactt ggatattctg aatatgtaga tgatatgaga attgaaggta
    35821 tgatttatgg ttcagcagtt agagctaagt atcctagagc tttagttaaa aaaatagata
    35881 tagaagaagc aaaagcttat ccaggagttg ttgcagttct aacagctaaa gatattccag
    35941 gaactataaa ggtagggcac ttaaagaagg attgggatac tttaatacca gaaggtgaaa
    36001 taactagata cttaggagat ggaatagttt tagtagcagc agaaactaga gaagcgttag
    36061 aagaggcaaa aaagctagtt aagatagatt atgaggaatt aacaccatta agtactacag
    36121 aagatgcgct tagagaagat gctccaaaga tacacgagac aggtaatata cttgttaagg
    36181 aacatttagt aagaggaaac gctaaagaaa agatagaaaa ttcaaaatat gttgtaacta
    36241 aaagatatac tacacctgct acagaacacg catttttaga gcctgaatgt gcagtagctg
    36301 gtccttatga agaggatgga gtaataatct attcaacaga ccaaggggtg tttgccactc
    36361 aacatgaatg tgcagatatg ttagggctac catatgagaa ggtaagagtt ataaacaaaa
    36421 tggtaggagg aggatttggt ggaaaagaag acatgagtgt tcaacaccat gcagccttac
    36481 ttgcatacca tactaagaga tttgtaaaag tacagttaac aagaaaagaa agtattattg
    36541 ttcatccaaa acgacatgcc acagaaatgg aatttacgac agcatgtgat gaaaatggat
    36601 acttaacagg tatgaaggca acgataatat ctgacacagg tgcatatgcc tctcttggag
    36661 gaccagtgct acaaagactg tgtactcatg cagcaggacc atataattat caagatattg
    36721 atataacagg aacagcagta tatactaata accctcctgg aggagcattt agaggatttg
    36781 gagttacaca gagttgtttt gcaactgaat gtaatctaaa tttacttgct gaaatggttg
    36841 gaatatctcc ttgggagata agatataaaa atgcaataag acctgggcaa gttttaccaa
    36901 atggtcaatt tgcagatgaa ggaacagcaa tggcagagac actagaagct gtaaaagaag
    36961 agtatgaaaa gcatccacgt gcaggtatag cttgtgctat gaaaaatagt ggggttggag
    37021 ttggtatacc tgatgttgga agatgtagat tggtgataaa agatggcaaa gtccacataa
    37081 gaacaagcgc ttcttgtata ggtcaagggc ttggtactgt tatgactcaa gtagtttgtg
    37141 aaactgctag cctacttcct gaaaatgtag tgcatgaagt tccagatact catttaacac
    37201 cagactctgg taatactacg gcttcaagac agacagtatt tacgggagaa gctactaagg
    37261 ttgcgtcaaa taagttaaaa gaagctttag actctggcaa aactttactt gatttagagg
    37321 gagaagagtt ttatggagaa tatgaaagta ttacagataa aatgggttgt gacaaagaaa
    37381 atccagtaag tcatgttgct tatggatatg caacacaagt tgtaattctg gatgaaaatg
    37441 gaaaattgga aaaagtagta gcagcacatg atgttggaag agctataaat ccaatcagtt
    37501 tagagggtca aatagaaggt ggagtagtta tgggtcttgg atatgctcta accgaagatt
    37561 acccagtagt taactctgtt cctacagcta agtttggtac attaggttta tttaaatcta
    37621 cagatatacc agaaatacaa gctttaatag ttgaaaagaa caaagattta ttggcttatg
    37681 gggcaaaagg tgtaggagaa atttgtacag ttcctactgc accagcagta caaaatgcct
    37741 attatgtatt tgataaagaa tttagaactg aactaccact acaaaatact ccatatagta
    37801 aaaaaaagta acaaaaaaaa gaaatattaa ataaaaaaat agatgaaatt gtaaataaaa
    37861 aaatagatga aattgtaaat aaaaaaaacc cttttgcata aggtatattt taaatgatag
    37921 aatatacata tacaggaggg tttttctttt ataaaataag tttaataaaa ttgagaggtg
    37981 agcagaatga attttaataa tagtctagaa ttaactcagt ctcagaagct aataatgact
    38041 acacaactta agcaatcatt atcaatatta aatatgagta agttagagtt agaagaagag
    38101 ataaaaagag aagcagagaa taacccatta gttgaagtcg agaaaagtgg ggagataaat
    38161 tgggaagagt atataaaaga catggatagg tcaagacgtt tagatagaac agagataagc
    38221 tataatcctg ataatgaagt taatctagaa aatttagtta aatattcgtc aaatttatat
    38281 gaagatttaa aatttcagat aagcctgtat aaacttacag acaaagaatt ggaagtctgt
    38341 gagtatataa ttgatagttt agatgaggat ggatatttaa gaactgagga aaaagtaatt
    38401 gttgatacct taaagataga tgaagaactt tttgaaaaat gtttaattag tgttcagcag
    38461 ttggaaccta gtggagtagg ggcaagaagt ctttcagaat gtctgatgat acaaatggct
    38521 agtttaggaa tatataatga aattttagag gaaatcgtta caaaagactt aaatttaatt
    38581 gcaaataata aatacaaaga gataagtaaa aagtacaata tgtcattaca aaaatgtgtt
    38641 gaactaataa acataattaa gacattagac ccaaaaccag gaagaacttg ttcagtagag
    38701 aaaagtgtat acattcaacc tgatgtaaca gtagaaaaaa ttgatgatga atttattgta
    38761 tatttaaatg aaaaagattc ttatcaaata agaatcaata gttattataa agacatatta
    38821 aaaaattcac agtctgatga aagtgcaaaa gaatttataa aagaaagact aaattcagca
    38881 acaggactta tgaaaaatat agagagtagg aaaactacag tacttaagat tgcagaagag
    38941 atagtaaaag ctcaagatga atttttgaga aaagggacaa aatatataaa acctttgaaa
    39001 atgaaagaca tagcagaaaa attagaattt catgaatcta ctataagtag aggtgtaaat
    39061 ggtaaatata tgttaacacc ttttggtgta tatgaattta gatacttttt cagtagtgcc
    39121 attgagactg agaataatga aatggcatct agtacaagca taaaaaaaat tataaaggaa
    39181 acaataaaag acgaaaataa aaagaaacct ttaagtgatg accatatatc taagttacta
    39241 aaagaaaaag gaatcaatgt tgcaagaaga acagttgcca aatatcgtga agaattagga
    39301 atcccatctt caagtaagag aaaagaatac taataagtaa aagacaaata tcagtatggt
    39361 aaggggaaaa ggctaattaa aagatgaatg tagattgggc taaaaatttt atgaagtaaa
    39421 ataatagaat atggaaaagt attaaaatgc tataagatat taaaaaactg gggaacatta
    39481 aaacattaag atagcaaaga acataaaaaa ctaaagaaga ttaaaacatt aaagaacatt
    39541 agagctttga ggaatattag aactttaaag aatattaaaa tttataaaat ataaaaatac
    39601 taaaaagaat tgaaaaactg gggaacatta aaatattgta aaatgctaaa aaactagaaa
    39661 acataattaa ataagtgctt ataaagaatg attaaattaa ataagtgttt gtaaagaata
    39721 attagatatt agaaaaagaa gaatacctag ataaataaac tattattaaa tttaataatc
    39781 ataaaaggaa aacacagggt ataaataaaa atcttaacta gataaaagta aacttagttg
    39841 tagataaaaa taactagaaa taaaagtaac tagaaataaa attatttata gtaataaata
    39901 ttagaatgaa aatataaaaa aatatatata aaaagcctta tataaaaaga aaaaatactg
    39961 tgatttgaaa atggtggaga aatattttat aaaataaaaa aataaaaaag tttttgaaaa
    40021 actattgaaa tctatacgca aatcctataa aataaaaata tcttgtggga cttaaataaa
    40081 cactagggac gaaaaaagtc ccgaaaggaa agcaataagg agtatgttat gaagaactta
    40141 ttagaaattc aaaagaaatt gattccacag gcaattgaac ttatggaaag aagatactct
    40201 attttaagac aaatatcttt aagtgaacca ataggtagga gaacattatc aaatgtatta
    40261 gagattagtg aaagaataat caggtcagaa actgagtttt taaaagaaca aggacttata
    40321 gacgtagctg tatccgggat gactatcact aaagaaggtt ctgatttgtt agataaactt
    40381 aaggacatca tgagtgacat catgggattg tctaagttac aagatagagt cagagaaaag
    40441 cttggaataa aaagggtaat attagttcct ggaagctgtg atgaaaatgg aagtttacta
    40501 aaagatgtaa ctagatatgg atgcgagtat ttcttaggta ttttaaaaga tggagatatc
    40561 gtttcaataa caggaggaag cactatgttg gagttttcaa acgttattaa aactgataag
    40621 agatacccaa actctacaat agttccagct agaggaagta tgggaactga tgtagagatg
    40681 caatctaata atatagttgc ttcgataagt aagaaattac attctaattg caaactttta
    40741 catataccag atgagttgga tgaaagtgca atgaaaactc ttagccaagt gccagagatt
    40801 aagacaactt tggagtatat taaaagaaca gacattttag tttttagtat aggtagagca
    40861 gatgtcatgg caaagagaag aaaacttcca gaagacaagg ttgatgagat attatctaaa
    40921 aatgcagttg gagaagcatt tggatattat ttcaacaaag atggagaaat agtttacaaa
    40981 ctgaacactg ttggaattga tttagaaaca gttaaacaag taaaagaaac tattgcaatc
    41041 tttgcaggta ctgaaaaagt agaagcctta ttagctatat caaatatgca taaaaacatg
    41101 gttcttgtaa cagatgaaga aagtgcttat aagatattgt cattattata aaagtcaaga
    41161 cctagcacag agctagaaaa catacattta ctttatagga ggtagcgaaa atggttaaag
    41221 tagctataaa tggatttgga agaataggaa gattagcatt aagaaaaatg atggagcaac
    41281 aagataagtt cgaagttgtt gcaataaacg acttaacaga cgctaaaatg ttagctcatt
    41341 tatttaaata tgatacagct caaggaagat ttaacggaga aatagaagtt aaagaaggtg
    41401 cttttgtagt aaacggaaaa gaaataaaag taacagcaga aagaaatcct gctgacctac
    41461 catgggctga attaggagta gatatagtat tagaatgtac aggattcttt acatctaaag
    41521 ataaagctga agctcatata caagcagggg ctaagaaagt tgttatatct gcaccagcta
    41581 ctggagattt aaaaacaata gtattcaaca caaacagtga tatattagat ggttctgaaa
    41641 cagttatatc aggagcttct tgtactacta actgtttagc tccaatggct aaagtattaa
    41701 atgataaata cggtatagaa aaaggattaa tgactactat acatgcatac actaatgacc
    41761 aaaatacttt agatggtcct catccaaaag gtgatttaag aagagctaga gctgctgctg
    41821 gaaacatagt tccaaacact acaggagctg caaaagcaat aggcttagta ataccttcat
    41881 taaaaggaaa attagatggg gcagcacaaa gagtaccagt tgtaactggt tcaataactg
    41941 aattagtttg tacattagga aaaaatgtta cagttgaaga aataaatgca gctatgaaag
    42001 aagcttcaaa cgaatctttc ggatatactg aagaaatgtt agtatcttct gacataatcg
    42061 gaataagcta tggttcatta tttgacgcta ctcaaacaaa agttatggaa gtagatggaa
    42121 aacaattagt taaagttgtt tcttggtatg ataatgaaat gtcttatact tctcaattaa
    42181 taagaacttt aggatacttt gcacaattag caaaatagtc agttttgaga aaatagaaaa
    42241 gtagattgac aaagtccgag tttgtagttt aatactacgg actcaggact ttttttaaga
    42301 aaaaatatat aaaatacacc taaataatgt tacatactat atataaaaat atgataatat
    42361 gatatattta atagggtaaa agtatgtact atattatact tggcaataaa aaattacttt
    42421 attaaaagga gagatattta tgtcaatgct taacaaaaaa actatagaag atattgatgt
    42481 gtgcggaaaa aaagtattag taagatgtga ctttaatgtg ccattacaag atggtgtaat
    42541 tactgatgaa aatagattga acggagcttt accaactatt caatatttaa tttctaaagg
    42601 tgcgaaagtt atattatgct ctcacttagg aaagccaaaa ggagaagcta agccagaatt
    42661 atctttagca ccagtagcaa agagattatc tgagatgtta ggaaaagaag ttgtatttgc
    42721 agcagacgat aatgttgtag gagaaaatgc aaagaaagca actgaaaaaa tggaaaacgg
    42781 agatgtagta ttattagaaa atactagata tagaaaagaa gaaactaaaa acgaagagaa
    42841 tttctctaaa gaattagcat cacttgctga aatatttgta aatgatgcgt ttggaactgc
    42901 tcatagagca cattgctcaa cagttggtgc aggtgagttc ttacaagaga gagtttgtgg
    42961 atacttaatt caaaaagaat taaaattctt aggagaagct gttgcaaacc ctgtaagacc
    43021 atttacagct atacttggag gagctaaagt ttctgataaa ttagctgtta taaacgaatt
    43081 attagaaaaa gttgataatc taataatagg tggaggaatg gcttatacat tcttaaaagc
    43141 tcagggttat gaagtaggaa cttctttact tgaaatagat aaagttgaat atgctaaaga
    43201 aatgatggaa aaagctaaga ataagggtgt taacttatta ttaccagtag acgttgttat
    43261 ggcagaccac tttgcaccag atgctactcc aatagtaaca gaagatgcta atgtaaaaga
    43321 agattacatg ggactagata tgggacctaa gactatagca aactttgtaa aaactataaa
    43381 agaatcaaaa actgtagtat ggaatgggcc aatgggagta tttgaatttg aaaactttgc
    43441 aaatggtaca ttatcagtag ctagagctat ggcagagttg actgatgcaa ctactgtaat
    43501 tggcggtgga gatagtgcag cagcagttaa tcaattagga tttggtgaca agatgactca
    43561 cgtatcaaca ggtggaggag catcactaga atttttagaa ggtaaagaat taccaggtat
    43621 tgcagcatta gataataaat aaactaatat atagaggaga ttgtaaaatg agaaaaccta
    43681 taattgcagg aaactggaaa atgcataaaa caataaaaga agctttagaa tttgtaaatg
    43741 aagttaaaga taaagtaaat tctgataaag tagaagctgt tatatgtgca ccatttactt
    43801 tattaaagga tttaaaagaa gctactaagg gtacaaatat aaagataggt gctcaaaata
    43861 tgcactttga agaaaaagga gcatttacag gagaagtttc acctcttatg ttaaaagaaa
    43921 tagatatgga ctatgttgta ataggacatt cagaaagaag acaatacttc aatgaaactg
    43981 atgaaactgt aaacaagaaa gttttaaaag cattagaagt aggaatagac ccaatattat
    44041 gtgttggaga aactttagaa caaagagaag ctggaaaaac taaagatgtt tgtaaagttc
    44101 aagttgaaaa agctttagaa aatgttctta aagatgattt agcaaaagta gttgttgcat
    44161 atgagccaat ctgggctatt ggaactggta aaactgcaac tgctgaagat gctaatgacg
    44221 ttatatctta tataagagaa gttataaaag gattatatgg agaattagct aatgaagtta
    44281 gaatacaata cggtggaagt gttaaacctt caaatgtagc cgaaataatg ggtcaaagtg
    44341 atatagatgg agctttagta ggtggagcta gtttagctag taatgattac ctagaccttg
    44401 ttaatttcta aggagtgaaa tgatgaaaaa accagttgct ttaattatta tggacgggtt
    44461 tggatacaat aaggatgtta agggaaatgc aattgctgag tccaaaactc ctaatttaga
    44521 tagaataaaa aaagagtatc caaacacatt gataaatgct agtggtcttg atgttggtct
    44581 acctgatggt caaatgggaa attcagaagt tggacacact aacataggtg caggtagaat
    44641 agtatatcaa gatttaacta gaataacaaa atctataaaa gatggtgact tttttactaa
    44701 taaagtttta tgtgaagcaa tggataatgc aaaagaaaat tctcttcatg taatgggact
    44761 tttgtctgat ggaggagtac attcacatat agaccatctt aaggctataa tcaaaatggc
    44821 aaaagataaa ggcgtacaaa aggtgtatgt tcacgcattt actgatggta gagatactga
    44881 ccctcaaagt gcattagaat atgccaaaga agtacaagct agtatggatg aaattggagt
    44941 aggtgaattt gctacagttt ctggtagata ctacgctatg gatagagata agagatggga
    45001 aagagtagaa cttgcataca atgccatggt tagaggtatt ggtgaaaaag caaattctat
    45061 tgaggaagct attcaaaact cttatgatga tggaaaaaat gatgagttta taatgccaac
    45121 agttattatg aaagatgata aaccagttgg aagtataaag gaaaatgatt caattatatt
    45181 cttcaacttt agaccagata gagctagaca aataactaga gctttagttt gtgaagagtt
    45241 tgatggattt aaaagagaag atattaaaaa cttctttgta tgcttaacag agtacgatat
    45301 aactatagag aatgtgcata tagcatttgg acctcaatct ttagccaaca ctctaggtga
    45361 gtatctagct aaaaatggaa aaactcaact tagagctgct gaaactgaaa agtatgcaca
    45421 tgtaactttc ttcttcaatg gtggagtaga agagccaaat aaaggtgaag aaagactatt
    45481 gataccttct ccaaaggttg caacttatga tttaaaacca gaaatgtcag catatgaatt
    45541 gacagataaa gctttagata aattaggaga agataagttt gactttatag tattaaactt
    45601 tgcaaatcct gatatggtag gtcatacagg aagtatagaa gcggctataa aggctgttga
    45661 aactgtagat acttgtgtag gaagattgat agataaaata gtagagttag gtggaagtgc
    45721 aataataact gctgaccacg gaaatgctga atatatgtta gacccagaaa caggaaaaac
    45781 tgtaacggct cactctatta atccagtacc atttattgta gttggtcaag aatatgaaag
    45841 tgcaaaatta ttggatggag gaagattatc tgatatagca ccgactgttt tagatatgat
    45901 gaagttagaa aagccagaag aaatgactgg tcattcactt atttcaaaat agtcgattct
    45961 gataagtaga gaataatata taaactacaa tataaattta aaaataatgc agtttaggtt
    46021 tattttgtaa agtttagact ttatg
//
LOCUS       DACULQ010000009        42056 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.009, whole genome shotgun sequence.
ACCESSION   DACULQ010000009 DACULQ010000000
VERSION     DACULQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42056
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.009"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            173..3121
                     /locus_tag="JA154_02050"
     CDS             173..3121
                     /locus_tag="JA154_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAT7994640.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3099..3563
                     /locus_tag="JA154_02055"
     CDS             3099..3563
                     /locus_tag="JA154_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT7994641.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3676..3978
                     /locus_tag="JA154_02060"
     CDS             3676..3978
                     /locus_tag="JA154_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAT7994642.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            4055..5404
                     /locus_tag="JA154_02065"
     CDS             4055..5404
                     /locus_tag="JA154_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086506.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAT7994643.1"
                     /translation="MEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGLTVGVG
                     FIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALIIPIGL
                     VVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFASVANMVIVLVMAD
                     LTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQKKFGL
                     FGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLPVSEAA
                     QEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVLPFADL
                     AVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFKMPEGA
                     TMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKLSIEE"
     gene            5439..5912
                     /locus_tag="JA154_02070"
     CDS             5439..5912
                     /locus_tag="JA154_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994644.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YVKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            6084..6734
                     /locus_tag="JA154_02075"
     CDS             6084..6734
                     /locus_tag="JA154_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994645.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6908..7840
                     /locus_tag="JA154_02080"
     CDS             6908..7840
                     /locus_tag="JA154_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAT7994646.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8114..8827
                     /locus_tag="JA154_02085"
     CDS             8114..8827
                     /locus_tag="JA154_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAT7994647.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8824..9309
                     /locus_tag="JA154_02090"
     CDS             8824..9309
                     /locus_tag="JA154_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAT7994648.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9404..9612
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9676..11391
                     /locus_tag="JA154_02095"
     CDS             9676..11391
                     /locus_tag="JA154_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAT7994649.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11513..12958
                     /locus_tag="JA154_02100"
     CDS             11513..12958
                     /locus_tag="JA154_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Proline--tRNA ligase 2"
                     /protein_id="HAT7994650.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13398..14879
                     /locus_tag="JA154_02105"
     CDS             13398..14879
                     /locus_tag="JA154_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAT7994651.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            15100..15255
                     /locus_tag="JA154_02110"
     CDS             15100..15255
                     /locus_tag="JA154_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994652.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15400..16797
                     /locus_tag="JA154_02115"
     CDS             15400..16797
                     /locus_tag="JA154_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAT7994653.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16800..17195
                     /locus_tag="JA154_02120"
     CDS             16800..17195
                     /locus_tag="JA154_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HAT7994654.1"
                     /translation="MEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRKVNDLHK
                     LAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKHATGFEA
                     LVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17301..18059
                     /locus_tag="JA154_02125"
     CDS             17301..18059
                     /locus_tag="JA154_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase (FAD)"
                     /protein_id="HAT7994655.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            18063..18809
                     /locus_tag="JA154_02130"
     CDS             18063..18809
                     /locus_tag="JA154_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAT7994656.1"
                     /translation="MNNLASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMA
                     KDKNIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIIL
                     DEITDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIV
                     NTIKRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMP
                     MIGNVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            18812..19339
                     /locus_tag="JA154_02135"
     CDS             18812..19339
                     /locus_tag="JA154_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAT7994657.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            19389..20051
                     /locus_tag="JA154_02140"
     CDS             19389..20051
                     /locus_tag="JA154_02140"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086520.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HAT7994658.1"
                     /translation="MGEIYMLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIIT
                     KYRNFVKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQI
                     ITAIKTATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKN
                     IESKMNELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENR
                     ND"
     gene            20125..21318
                     /gene="tuf"
                     /locus_tag="JA154_02145"
     CDS             20125..21318
                     /gene="tuf"
                     /locus_tag="JA154_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086521.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAT7994659.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVE
                     DVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGD
                     NIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRPQF
                     YFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGVV
                     ATIIE"
     gene            21690..22001
                     /locus_tag="JA154_02150"
     CDS             21690..22001
                     /locus_tag="JA154_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAT7994660.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            22093..22722
                     /gene="rplC"
                     /locus_tag="JA154_02155"
     CDS             22093..22722
                     /gene="rplC"
                     /locus_tag="JA154_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086537.1"
                     /note="binds directly near the 3' end of the 23S rRNA,
                     where it nucleates assembly of the 50S subunit; essential
                     for peptidyltransferase activity; mutations in this gene
                     confer resistance to tiamulin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAT7994661.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            22752..23375
                     /locus_tag="JA154_02160"
     CDS             22752..23375
                     /locus_tag="JA154_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAT7994662.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            23375..23665
                     /locus_tag="JA154_02165"
     CDS             23375..23665
                     /locus_tag="JA154_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAT7994663.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            23695..24525
                     /gene="rplB"
                     /locus_tag="JA154_02170"
     CDS             23695..24525
                     /gene="rplB"
                     /locus_tag="JA154_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086540.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAT7994664.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            24560..24841
                     /locus_tag="JA154_02175"
     CDS             24560..24841
                     /locus_tag="JA154_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAT7994665.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            24873..25208
                     /locus_tag="JA154_02180"
     CDS             24873..25208
                     /locus_tag="JA154_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAT7994666.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            25231..26046
                     /gene="rpsC"
                     /locus_tag="JA154_02185"
     CDS             25231..26046
                     /gene="rpsC"
                     /locus_tag="JA154_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086543.1"
                     /note="forms a complex with S10 and S14; binds the lower
                     part of the 30S subunit head and the mRNA in the complete
                     ribosome to position it for translation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAT7994667.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            26082..26513
                     /locus_tag="JA154_02190"
     CDS             26082..26513
                     /locus_tag="JA154_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAT7994668.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            26515..26718
                     /locus_tag="JA154_02195"
     CDS             26515..26718
                     /locus_tag="JA154_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAT7994669.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            26742..26996
                     /gene="rpsQ"
                     /locus_tag="JA154_02200"
     CDS             26742..26996
                     /gene="rpsQ"
                     /locus_tag="JA154_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086546.1"
                     /note="primary binding protein; helps mediate assembly;
                     involved in translation fidelity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAT7994670.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            27022..27390
                     /locus_tag="JA154_02205"
     CDS             27022..27390
                     /locus_tag="JA154_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAT7994671.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            27414..27719
                     /locus_tag="JA154_02210"
     CDS             27414..27719
                     /locus_tag="JA154_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAT7994672.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            27749..28291
                     /gene="rplE"
                     /locus_tag="JA154_02215"
     CDS             27749..28291
                     /gene="rplE"
                     /locus_tag="JA154_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086549.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAT7994673.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            28309..28494
                     /gene="rpsN"
                     /locus_tag="JA154_02220"
     CDS             28309..28494
                     /gene="rpsN"
                     /locus_tag="JA154_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086550.1"
                     /note="located in the peptidyl transferase center and
                     involved in assembly of 30S ribosome subunit; similar to
                     what is observed with proteins L31 and L33, some proteins
                     in this family contain CXXC motifs that are involved in
                     zinc binding; if two copies are present in a genome, then
                     the duplicated copy appears to have lost the zinc-binding
                     motif and is instead regulated by zinc; the proteins in
                     this group appear to contain the zinc-binding motif;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAT7994674.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            28527..28925
                     /locus_tag="JA154_02225"
     CDS             28527..28925
                     /locus_tag="JA154_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAT7994675.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            28956..29498
                     /gene="rplF"
                     /locus_tag="JA154_02230"
     CDS             28956..29498
                     /gene="rplF"
                     /locus_tag="JA154_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086552.1"
                     /note="ribosomal protein L6 appears to have arisen as a
                     result of an ancient gene duplication as based on
                     structural comparison of the Bacillus stearothermophilus
                     protein; RNA-binding appears to be in the C-terminal
                     domain; mutations in the L6 gene confer resistance to
                     aminoglycoside antibiotics such as gentamicin and these
                     occur in truncations of the C-terminal domain; it has been
                     localized to a region between the base of the L7/L12 stalk
                     and the central protuberance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAT7994676.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            29534..29902
                     /locus_tag="JA154_02235"
     CDS             29534..29902
                     /locus_tag="JA154_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAT7994677.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            29923..30432
                     /gene="rpsE"
                     /locus_tag="JA154_02240"
     CDS             29923..30432
                     /gene="rpsE"
                     /locus_tag="JA154_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086554.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAT7994678.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            30447..30632
                     /locus_tag="JA154_02245"
     CDS             30447..30632
                     /locus_tag="JA154_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAT7994679.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            30665..31108
                     /locus_tag="JA154_02250"
     CDS             30665..31108
                     /locus_tag="JA154_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAT7994680.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            31152..32420
                     /locus_tag="JA154_02255"
     CDS             31152..32420
                     /locus_tag="JA154_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAT7994681.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            32437..33087
                     /locus_tag="JA154_02260"
     CDS             32437..33087
                     /locus_tag="JA154_02260"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086558.1"
                     /note="essential enzyme that recycles AMP in active cells;
                     converts ATP and AMP to two molecules of ADP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAT7994682.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            33088..33834
                     /locus_tag="JA154_02265"
     CDS             33088..33834
                     /locus_tag="JA154_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAT7994683.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            33856..34125
                     /locus_tag="JA154_02270"
     CDS             33856..34125
                     /locus_tag="JA154_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HAT7994684.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            34163..34381
                     /locus_tag="JA154_02275"
     CDS             34163..34381
                     /locus_tag="JA154_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAT7994685.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            34417..34530
                     /gene="rpmJ"
                     /locus_tag="JA154_02280"
     CDS             34417..34530
                     /gene="rpmJ"
                     /locus_tag="JA154_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAT7994686.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            34683..35054
                     /locus_tag="JA154_02285"
     CDS             34683..35054
                     /locus_tag="JA154_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAT7994687.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            35090..35488
                     /locus_tag="JA154_02290"
     CDS             35090..35488
                     /locus_tag="JA154_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086564.1"
                     /note="located on the platform of the 30S subunit, it
                     bridges several disparate RNA helices of the 16S rRNA;
                     forms part of the Shine-Dalgarno cleft in the 70S
                     ribosome; interacts with S7 and S18 and IF-3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAT7994688.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            35520..36143
                     /locus_tag="JA154_02295"
     CDS             35520..36143
                     /locus_tag="JA154_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAT7994689.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            36221..37168
                     /locus_tag="JA154_02300"
     CDS             36221..37168
                     /locus_tag="JA154_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAT7994690.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            37189..37530
                     /locus_tag="JA154_02305"
     CDS             37189..37530
                     /locus_tag="JA154_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAT7994691.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            37650..38483
                     /locus_tag="JA154_02310"
     CDS             37650..38483
                     /locus_tag="JA154_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086568.1"
                     /note="with CbiNQ forms the ABC transporter for cobalt
                     import; Mycoplasmas have two adjacent copies of this gene;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HAT7994692.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            38471..39337
                     /locus_tag="JA154_02315"
     CDS             38471..39337
                     /locus_tag="JA154_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086569.1"
                     /note="with CbiNQ forms the ABC transporter for cobalt
                     import; Clostridia have two adjacent copies of this gene;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAT7994693.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            39331..40134
                     /locus_tag="JA154_02320"
     CDS             39331..40134
                     /locus_tag="JA154_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAT7994694.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            40166..40897
                     /locus_tag="JA154_02325"
     CDS             40166..40897
                     /locus_tag="JA154_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAT7994695.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            41017..41448
                     /locus_tag="JA154_02330"
     CDS             41017..41448
                     /locus_tag="JA154_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAT7994696.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            41477..41869
                     /gene="rpsI"
                     /locus_tag="JA154_02335"
     CDS             41477..41869
                     /gene="rpsI"
                     /locus_tag="JA154_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086573.1"
                     /note="forms a direct contact with the tRNA during
                     translation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAT7994697.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT        17111 a         4678 c         8344 g        11923 t
ORIGIN      
        1 ttgtttttaa aaaaagtatt atttgtaaaa tataaaaaaa aatttcttaa tatttgaata
       61 aaagaatagc ttaatttaaa tgttattact ttataaaaaa ataataagta caaaaaaaac
      121 aatatatggc atataagttg ctatataaat aataaggagg gaacaattat aaatgaggca
      181 aagaataaaa aacttaattg aaaatgaaga taagaaaaat cctttaacag atgaagcgat
      241 ttcaagtagg ttaaatattc gaagagaaga tgttaccttt ttgagaaatg agttaaaaat
      301 agaagattca agacaaagaa gaaaagttgt tttaattaaa gctataaaag atattttaaa
      361 agaagaaaaa actataaata aaaatgatat aacaagaaga ttaaacagca ttgggtttac
      421 agtatcaaga tttacagtca tacaatactt aaaagagatt caagattctg gtgacctaaa
      481 acttgataaa gagagtgatg ataaaaaaga agaaataatc aataatagag aaaataaaga
      541 tatagcattt gataaactta taggtagtag tggtagctta agtacaatta taaaactagc
      601 caaagcagca attctttatc cacctcatgg tcttcatact attattatgg ggccaactgg
      661 tgttggaaag agtgagttag cagagtgtat gtataaattt gcagtagaat catccagatt
      721 ccctaagaat gcaccattta tagtttttaa tgcagcagat tattcagaga atccacagct
      781 tttgctatca cagttatttg gacacgttaa aggtgctttt acaggagctg atgagaacaa
      841 agaaggatta gtatcaaagg cagatggagg aatattgttt atagatgaaa tacatagatt
      901 acctcatgaa ggtcaagaaa tactatttca actgattgat aaaggtatgg tcagaaagtt
      961 aggagaaaca agactaacac ataaaataga tgtaatgatt atttcagcaa cttctgagcc
     1021 aatagattct catttactta atacatttaa aagacgtata cctgtaacaa ttgaaatacc
     1081 agaattagtt gcaagaccat taaatgagag gtttgacatt ataaataatt tcttcttaat
     1141 agaagctcaa agaatgggag caaacataca tataaagtca gatgtattaa aagctcttat
     1201 gctttacgat tgtataggta atgtaggtca acttagaagt gatattcaag tagcttgtgc
     1261 taggggacta ttaaaccaac ttacaaacaa actaaaagaa gtaaatataa gcatatcaga
     1321 tgtacctgga tatgtaaaac aaggtcttat aaaaatcaga aattgtagag ggaaaataga
     1381 aaattatgta gatggggatt taattataga ctcaaatctt caaggtgaga tagaaaagaa
     1441 aaatgaagat atatatactt ttccagatga aatatacaaa cttactgaaa gaagacatat
     1501 tgatttattg gaacaaggtt tagattttga tgttataaat agaatcattg gaggagaatt
     1561 agaaagttgc attcaaaaat acataaaaca agttaaaaaa gtttctatta gtaacaatct
     1621 tccagatata agtccaattg ttggaaaata tatcgtagag ctaacttatg aagtaatcaa
     1681 aatagcatca caatacctaa gttcactaga accgaatcta catttatgtt tagcagttca
     1741 cctaaaagca gcttttgaga gattaaaaga aggaaaagta atatcaaatg ctcatttgaa
     1801 agaaataata ggaaattatc cagtagaata ttctgtagca gaaaaggtag ctcaatattt
     1861 aaaggaaaga tataacataa acttaccaaa agaagaaata ggtataatag caatatattt
     1921 taaaatgagt agtagaagag atatgtctga acaaaaaaag ataggcgtac ttcttatggc
     1981 tcacggaaga gtagcatctt ctgtatgtga ggttacaaat aaactattag gaattaatca
     2041 cgcaaggtat ttagatatgc catttgagaa gaagccggaa gaaatgattg aagaagctac
     2101 tacaataata aaagatattg atgaaggtag aggtgtgatt atattagtag atatgggttc
     2161 actttcatat tttggagata ttatttctgg aaagactgga attaatataa ggacaatatc
     2221 tagatttgat acattgcttt ctatagaggt tatacgaaga gcagttttac cagatttaac
     2281 aatagacgag atagttgatg aagttaaagg tatagagcca caagtaaaag aaaaggttag
     2341 taaatgtaac aatacaaaag tagattttag aaaacctata attttaacat tatgtattac
     2401 tggattaggg ggagcttgta agataaaaaa aatcttagag gaaaatatta aaagtataac
     2461 tgaaaaggtt gatatagaac ctataggtat gattgaatat ccaagtcttg atttagagat
     2521 aaaacaaata agcaatgata aaaatgtaat cgccatagtt ggaacaattg accctcagat
     2581 agcaaatatc ccttttttta gtctagtaga cataaatgat aaagcgaagt taaatcaaat
     2641 aaataaccta attgatacaa atcaaattgg tgaaacaaat aaaaaatatg aattatcaga
     2701 tttaatacat aaagaagata tatttgtgga tttacaggtt aaatcaaaag aagaagttct
     2761 aaaatatatg agtaaaaaac ttagagagaa aggttatgtg acaaaaaatt tctataaaaa
     2821 agtagtggaa agagataact tatttccaac tgaattatca gatgaaatag caatacctca
     2881 cacagatgga atagatgtaa ttaggccagc tataagtata gccatcttaa agaagcctat
     2941 tgtgtggagt aaaagtaaag taaaaataat acttttatta gctgtagacc aaaagtgttt
     3001 taatccatta agcactctac tctcatttat agaaacagat gaatttaaga agataaaaaa
     3061 cattaaagat agagatttta ttagggagat gatattagat ggagtcaatg aaaataatta
     3121 gtagcaattt aatttttaaa aatattgaag tgtctaacaa tgaagatgca ttaaagtttt
     3181 tgggacaacg tttgtttgac gaacagtatg taaaagaaag ttacatacaa gctgttgtag
     3241 caagagaaaa aaagtatgct acagggttac caacagaaat atacggagtg gcaataccac
     3301 atacagatat agtacatgta aatgagccag gaatagcaat tggaatactg aataaacctg
     3361 taaaatttat aatgatgggt acagacgata ctgaaattga tgtaaaagtt gtatttatgt
     3421 tagcagtgaa agagccacaa gaacaattgc aactattaga aaaacttatg actatatttc
     3481 aagataagaa tatactaaat aatattgttg acttaagtga agaaagtgtg agtgatttat
     3541 taaactctaa attgaaaaat taaacatgta aagtatatta aaaatgtaaa attaaacatg
     3601 caaagtatgt taaaaaatat aaaaagatta ttaaagattt ctaactatag atatctttaa
     3661 aataaaataa tttcaatggg ggtgactaat gtggctaaaa agatattagt tgcatgtgga
     3721 acaggagtat gtacttcaac aattgctata aacaaattaa agaaagcact tcaagacatt
     3781 ggtaaattgg atatggttaa tattacacaa tgtaaagtag tagaagttgc atcaaaagca
     3841 cctgattatg atttaataat ttgtactaca caagtatcta gtagtataaa aacaccagtg
     3901 ataaatggat tacctttttt aactggtgtc ggaatggata aattaatcaa tgatgtatta
     3961 gaagagttag aattataatt aaaaaaatta aggaacatgg tctctaaaat cataagtttt
     4021 agagacttaa aattaaataa tggagggtct ttatatggag atattaaatt tcattgttgg
     4081 tctaggtgca caagtaatga tgccaataat aatatgtatt tttggtttga tattaggtac
     4141 aaaactagga aaatctttaa gagctggttt aactgtggga gttgggttta taggtcttaa
     4201 cactattata gctttgctaa cagataattt aggtccagct acacaacaaa tggttaagaa
     4261 tttagggcta agtttatcaa taatagatgt aggatggcca gcagcatcag ctatagcttt
     4321 tgcatcaaca gtaggggctt taattattcc aataggtctt gtggtaaata tagttatgct
     4381 aattactaat actactcaaa ctgttgatgt agacatatgg gattactggc attttgcttt
     4441 tacaggagct ttagtggcag gtgctactca aagtgtaatg tggggagtat ttgcatcagt
     4501 agcaaatatg gtaattgtat tggtaatggc agatttaact gctccaggta tagaggaata
     4561 tttaggtatg ccaggtatat ctcttcctca tggatttaca caggcatttg ttccaattgc
     4621 tatagtagta aataaattat tagacctaat accaggtata aataagattg agataaatgc
     4681 tgatacattg cagaaaaaat ttggattatt tggagaacct ttaataatgg ggtctgtaat
     4741 aggtgtaata attggtattg ctgcgaaata tgatataaaa ggaatattac aattaggtgt
     4801 aactatggga gctgtattaa tattaattcc aaagatggca gcattattaa tggaaggatt
     4861 attaccagta tcagaagctg cacaggaatt tattgagaag agatttaaaa acagaggcaa
     4921 gatttatata ggcttagatt cagcagtagg aataggtcat cctgttacat tatcagttgc
     4981 actagtgttg gttcctttga ctatacttat agcagctata ttgccaggaa ataaagtttt
     5041 accatttgca gatttagcag taattccatt tgcacttgta ttaatcgtac caattacaaa
     5101 gggaaatgtg tttagaacat taataatagg aataattata attacttctg gattattaat
     5161 cgcaactaac ttagctccat tatttaccca aatggcttta aatgctagtt ttaaaatgcc
     5221 tgaaggagca actatgattt caagtatttg tgatggtgcg aatccattaa gttgggtatt
     5281 tgtaaaagtt atgaactata aagttattgg aggagtagtt tttggagtta tagctttagg
     5341 aatggctatt tacaatagga atagaataat aaatgaaaat aaaaagctat ctatagaaga
     5401 ataaggttaa aacaatttta ttaaggagtg tataggttat gaaaaaatat aaatatgatt
     5461 ctaaaagata cttactcagt gttacaatac caggattttt tatgatttta atattgattt
     5521 atgcactgtt taataactat acaaatttta atttgaatat atatagtttg ttaataattg
     5581 tctgtactta tggtatattc aatactttta tatcatcaag taatcctaaa aaaattatta
     5641 ttgatagcaa atgtatacat tttacctcat ttatgactac acataagtat gaaattaaag
     5701 atatagaaaa gataagaata agagaatttt ataataaaaa aatttatgta aaaattaatg
     5761 atggaaacct ttttaaaggt aggtattgga taagaacaaa tatgtttaat aatagtatag
     5821 cattatatag ctatttcatt gagttggaag agtgcttata tccagattca ctaaaattta
     5881 gaaataaaaa atttgagaat aagaacatat aaaaataaaa aattgaattg aagcctagct
     5941 taatcttttt tgataagata taagtttgaa ctttatacta aatttttaga attttcaatc
     6001 aagacaataa cataatagct aagcaattag aacacaatta agatgtataa ggcatgtata
     6061 tgatgaatgg gaggatttaa ttaatgttat tagagaaatt aagaaaagaa gtattacaag
     6121 catctttaga ccttttaaac tataatttag tgacattaac aggaggaaat gtaagtggga
     6181 gagatgaaca aactggctat atagcaatca ccccaagtgg aatggattat agaaatctta
     6241 caccaagtga tattgttata gtagatgtag atggaaatat tatagatgga aaatggaaag
     6301 catcagtgga tttatcagac cacttgtata tatataagca tagagaagac ataaatagta
     6361 taattcatac acattctaca tactcaagtt gttttgctat tttaaatgaa cctatagaat
     6421 gtgcaagtac aactcttgca aatgaagtag ggggttcagt accagttgca aaattttcac
     6481 caccaacatc taaaaagatg ggaaaatgtg ttattgaagc aattggtgac aaaagagcat
     6541 gtcttttagc aaatcatggc gtaattgcag ttggtccatc tgtaggacat gctcttacag
     6601 ccgctgtaat gttagaagat tcagcaaaag tatattactt agcaaaatca attggcacac
     6661 cagtgctact gcctgatgaa gaaattcaaa gagcgagaga tgtatttttt aatgtctatg
     6721 gtcaggataa ataaattaga taaaatttta aaagtaatat gaaaatatag atattcaatg
     6781 atattaagga gatttataat gatataataa gtttaatagt taagaagtaa aatatgaaat
     6841 gtaatagtat ataaattcat ttaagactaa aaagaataag ataaaataaa aaggagtctg
     6901 tatttctatg tattacttat taaacaaaag cacaaatcct tactttaatt tggcattaga
     6961 agaatatcta tttttaaatg ataaatataa tgatgatatt ataatcatat ggagaaatga
     7021 agaatccatt tttataggta aaaatcaaaa tccatatcag gaagtatatc atgatgttat
     7081 tgaaaaggga gaaattccta ttttaagaag aatatctgga ggaggaactg tttatcatga
     7141 cttaggtaat atcaatatgt cattcataca aaaagatagg caacttcatg agatagattt
     7201 tttggagcat actaagttta tgcaaaagat gctttcaacc ttaggactag atgtatctat
     7261 aactgaaaga aaagatttat ttttaaatgg gaaaaaaatt tcaggctctg cacagtctat
     7321 aaaaagaaaa aattctcttt atcatggaac tcttctttac gattcagatt taaataaatt
     7381 aactaaatat ttaaattcaa ataaagcaac tgaatcaaat gcaactaaat ctgtttcaag
     7441 taaagttaca aatattaagt ttttactcga aaaagatata aattactttt tagattattg
     7501 tgttgaatat ttaaaatcta atataaagaa tataaaagaa cttgaattgg atgaagaaga
     7561 tattaaaagt gtatacaata taggagaaga taaatataaa aagatagagt ggacatatgg
     7621 taaaacgccg aaatttcaag tagtgctacc atttaataat gaaagtaaaa taaatataca
     7681 tgttaacaga tggaaagtat caaaattttt tatttcttat gatgataatg taattaatat
     7741 ggacaagttg ttagatttga atttctttaa agaagaaata aaagagagta ttttagaaaa
     7801 ttatccacat tacatagatt taatcaattt aatattttaa tataaaacct ctaatttgat
     7861 aaaattgtct tatatcaaac tagaggtttt taatattctt gcatattaac aaccattaca
     7921 aaattgtaaa attaaaaaca acactattga aaaatcaata tatattgtaa taaatatatg
     7981 tgtttttcat tagcaaatag acattaacta tttatacaac tgaaaattta atatataatt
     8041 aatcagatgg ttaaaataaa aatattaaaa gttttaataa atgatggtta aaaataaaat
     8101 acgaggtgat accatgtata gtgttataat tgttgcagcg ggaagcggaa ggagaatgaa
     8161 cttagatata aacaaacagt tcataaaatt aagagaaaaa gaaattattg ctcatacaat
     8221 tcaagttttt tatgaaaata taaatataga tgaaatagtg gtatgtataa aaaaagaaga
     8281 agaagatttt tttaaagaaa atataataaa taagtataac tttaaaaata taaaaatagc
     8341 atatggcgga aaagaaagac aggactcaat atataatggt ttaaaaaaat tagatgaaaa
     8401 ttgtgatata gtacttatcc atgatggagc aagaccattt gtcgaccata gaataatcaa
     8461 tgaatctata aaggtagcta aagaaaaaaa ggctgtggtt gtaggtgttc cagtaagtga
     8521 tactataaag atagtgtcag atggaacagt acaagaaact ccagaaagaa atttgctgtg
     8581 ggcagcacaa acccctcaaa cctttgaata taatctcata atagatgctt atgaacaagc
     8641 atataagaac aattattatg gtactgatga ttcaatgttg gtagaaaata taggtcaaag
     8701 tgtcactatg gtaatgggtt cttatgaaaa cataaaaata actagtccag aagatttaaa
     8761 tatagcagaa caaatattaa atatggaaaa aagagatgaa gttaacagta gaaggagaat
     8821 tatatgagaa taggattagg atatgatgta cacaaattaa cagaggatag aaaacttata
     8881 atcggtggtg tagagatacc acacgataaa ggtttgttgg gtcattctga tgctgatgta
     8941 ttgatacatg caataatgga ttctatttta ggtgctctag cattaggaga tatagggaag
     9001 catttcccag atacagatga agaatataag ggagcagata gtatgaaatt attagaacat
     9061 gtgtataatc taataacaag taaaggatat aaaattggta atatagacag tactataatt
     9121 gctcaaagcc ctaagatggc tccatatatt gagagtatga gaagtaatat atcaaaagtt
     9181 ttaaacacag acattgataa tataaacata aaggcaacaa cagaagaagg tcttggtttt
     9241 acaggagcaa aacagggaat agcttcacaa agtatttgtt tattattgtt gacaagtcag
     9301 aataattaat atattattag taataagttt aatggatatc aagtaatagg cctatataga
     9361 atatctataa tgtatgaatt taagtgaaat taatataaat ataaaattag ctatgataaa
     9421 gaaatagtaa aatgatgaaa tcttcacaga gagaaaacgg tggtgagagt tttcaagagg
     9481 aagtctttga acctgtcttt tagcttaaag gtataaaaat ctttacggta ggaaccgtta
     9541 tattcccaaa agagagctat tcatatagct aactagagtg gtaccgcgga aattaaacct
     9601 ttcgtctcta tacttgtata gagatgagag gttttttata ttttcaggaa gttaatattc
     9661 aggaggaatc tattaatgaa aatgtcaaaa atgtttatgc cgacattaaa agaaatccca
     9721 gcagatgctg agattacaag ccatcaatta atggttaggt caggcatgat aaaaaaaatg
     9781 acttcaggtg tttataacca attaccaatg gggttaagag tgttcaaaaa aattgaacaa
     9841 ataattagag aagaattaaa taaaaaagat tgtcaagaaa tactttgtgc agccttactt
     9901 ccttctgaat tatggaaaga gtctggaagg tggactgcta tgggtgaaga aatgtttaga
     9961 ctaaaagaca gaacagaaag agagtactgt cttgggccaa cacatgaaga agcattcact
    10021 gatataataa gacaagaaat tacttcatat aaacaattgc cactaaactt gtaccaaata
    10081 caagtaaaat atagagacga gagaagacca agatttggtg ttatgagaac taagactttt
    10141 acaatgaagg atgcatatag ttttgatgta gatgataaag gtttagataa gtcatatcaa
    10201 gatatgtttg atgcatatgt aagtatattt gatagatgtg gattggagaa tagccctgta
    10261 caggcagatt caggagctat aggaggttct acatcagccg aatttatggt taagtcagaa
    10321 gttggtgaag atgaagttgt attttgtagt ggatgtgatt atgcagccaa tgtagaaaga
    10381 gcagaatctt gtaatctagc atcacaaaaa gaagaaatga aagaattaga agaggttcat
    10441 acaccaggag cagctacaat aaaagagtta gaagaatttt taaaaacttc tccagataag
    10501 tttgctaaaa ctttagtata tgaggcagat ggaaaaacag ttgtagtcgt tgtaagagga
    10561 gatagagaag ttaatgaaat caaagtatct aatgccatag gttctgttat agaatttgct
    10621 cttgctacag atgatgtagt gagaaaagta actaatgcag aagtaggatt tgcaggtcca
    10681 ataggtataa atgctgacta tgtatttata gataaagaaa ttgttgaaca aagaaacata
    10741 gttgttgggg ctaataaaac tgagtaccat ataaaaaatg ctaactatgg aagagatttt
    10801 gaaggaatag ttggagattt tagaaatgtt caagaaggcg acaagtgtat agtatgtggt
    10861 aagccacttg aaatagctag aggtgttgaa gtaggacata tatttaaact aggaactaag
    10921 tactcagaat ctatgaatgc aaacttcata gacaaagatg gaaaatcaaa gcctatagta
    10981 atgggatgtt atggaatagg tgttgaaaga acagcagcag ctataattga acaacataat
    11041 gatgagaaag gtataatatg gcctttgtca gtagctccat accatgttgt gatagttcca
    11101 gcaaatatga aaaatgaaga acaaatatca atagctgaaa atatatataa tgatttgcaa
    11161 gctatgggtg tagaagtttt attagatgac agagatgaaa gaataggtgt caaattcaat
    11221 gattcagaat taataggaat acctatgagg attacagtag gtaaaaatat taatgaaggt
    11281 aaagtagaat ttaaacttag acataaagaa gataaagaga taattgacat agaagaaata
    11341 aatgaaaaag taaaggcaga gtttatcagg aataatgtta ggttaggata ataacttaaa
    11401 taagatattg tatagataat caaagttgta ctttatagac accattttat ctatatataa
    11461 ggatataaaa tggtgttttt ttataaaaca aataaatgag gaggtaatta aaatggcaaa
    11521 aaatgaaaaa caatttgttg aagaaataac aaagatggaa gatgattttc cacaatggta
    11581 cacagatgta ataactaaga ctgacttagt tgattatgca ccagtaaaag gttttatggt
    11641 tgtaaagcca tatggatatg cattatggga aaagatgcaa gaatttatgg ataaaaaatt
    11701 taaagaaact ggacataaaa actgttattt tccattatta ataccagaga gtttattaaa
    11761 caaagaagca gaacacgttg aaggttttgc tccagaagtt gcatgggtaa cacatggagg
    11821 aaataaaaaa ttagaagaaa gattatgtgt aagacctact tcagagacta taatatgtac
    11881 aatgtatgcg aaatggttaa aatcatatag agaattacca tatctttata atcaatggtg
    11941 ctcagttgtt agatgggaaa aatcaactag acctttctta agaacatctg agtttttatg
    12001 gcaagaaggt catacgttac atgaaacagc agaagaagca caagaagaaa caatacaaca
    12061 attagaagta tataaagcgc tatgtgaaga attacttgca atgccagttg tagctggtca
    12121 aaaaagtgaa agtgaaaaat ttgcaggtgg tgaaagaaca tatactatag aagctatgat
    12181 gcatgatggt aaagcattac aatcaggtac aagtcacttc ctaggtcaac actttactaa
    12241 ggcttttgat attacttttg cagatagaga aggaaattta gctaatccat atcatacatc
    12301 ttggggagca tctacaagac ttataggtgg acttataatg actcatagtg ataacagagg
    12361 tttagtatta ccaccaagag tagctccaat tcaagttgtt atagttccta tagctgctaa
    12421 aaaaggaaat gtaatggaga ctgttgataa aatatatgct gatttaaaag ctaagggagt
    12481 agctgtagaa gttgatgata gagataacta tacaactgga tggaaattta atgagtggga
    12541 aatgaaaggt gttccagtaa gagttgaaat tggaccaaaa gatatagaaa ataatcaagc
    12601 tatggtattt agaagagata ctttagaaaa ggattctatg cctttagaag gattagctga
    12661 tgctatatgt gatttatttg atgttataca caatgatatg tttgaaaaag caagaaaaca
    12721 tagagaagac aatacatcta tagttgaaaa tatggatgag tttagaaaag ctttagaaga
    12781 aaagccagga ttcataaaga ctatgtggtg tggagatgca gaatgtgaag ctaagattaa
    12841 ggaagaaaca ggagcaacta taagatgctt accttttgaa caagaaaact taggtcacaa
    12901 gtgtgtgtac tgtggaaaag aagcagatag tatggtagta atggcaaaag cttactaaaa
    12961 tacatttaaa taaaaactgg ggtagaaagt atttaatact ttatcccagt ttttttgttt
    13021 tttatgagta ataaaatgta tttgtaacta tgtcataaga taagtcatag atacttatat
    13081 taaaaagaat ataatagaga actattttta ataattacat attaatgaaa gcaaaatatt
    13141 tttgttatat agattttcaa taaagcaaac aataaatata aatgaatatt agtaaggagt
    13201 gcttttaatt tttagaaaat aaatgactta aaagaaagat ttatgaaatt aaaactagga
    13261 ttttctgata taataaacat gtgtattttt aaataaaaag gatattatta ttagatttga
    13321 ttaaaaataa acttgcaaaa caatgtacta gtttaatatt atagttatat tgaataaaat
    13381 atcaggagga agtaaaaatg aatgtaagag taaggtttgc accaagtcca acaggatttg
    13441 tacatatagg tagtttgaga actgctttat ataactattt atttgcaaaa aaaatgggcg
    13501 gagaatatat attaagagtg gaagatacag accaaagtag attagtagaa ggtgctatag
    13561 agaatatgct taatgctatg aaatgggctg gggtcaacca tagtgaaggt gttatattag
    13621 atgatagtgg aaaagtagta caaaaaggag aatatggtcc atatattcaa tctcaaagat
    13681 tagatatata ccaagaatat ataaagcaac ttttagatag tggaaaagct tattactgtt
    13741 tctgtacaaa agaaagactg gatgaagtaa gagatgctca aagagcagca ggagaaacag
    13801 ctaaatatga tggtcattgt aagaatcttt ctaaagaaga agtagaagcg aatataaaag
    13861 caggaatacc atatgttata agattaagat tgccagagaa tcatacaatt aagtttacag
    13921 acttagttag aggagatatg gaatttaata ctaatgactt agatgaccaa gtattaatga
    13981 aaacagatgg atttccaact tatcattttg cagtagttgt tgatgactat ttgatgaaaa
    14041 ttacacatgt tataagagga gaagaatggg tatcatctac tccaaaacat gtttatttat
    14101 atgaagcttt tggatgggaa gcacctgtat ttgtgcattt accaaatata cttaataaag
    14161 aaaagaaaaa attaagtaaa agacaaggtg atgtagctgt tgaagacttt aagaaaaaag
    14221 gatatttacc agagggatta gtaaactatg tagcattagt tggttggtct cctgaagata
    14281 atcaagaatt gtttacaatg gaggaattag aaaaagcctt ctctgtagaa agagtctcta
    14341 aaagtggtgg agtatttgat actgaaaaat taaattgggt taatcagcat tatataaaag
    14401 atggtgatga tgcttatcta acagacttag caataccatt tttaatagaa gatggattta
    14461 taacagaaga agaagctact aataaatatg atttcttaaa gagtatgata tcagtactta
    14521 aagagaaatt acaatatgta aaagaagtta ctgaacatgc aagtatattt tttggagata
    14581 aaatagaggt agaaactgaa gagggaagag agttcttaag attagaacat atacctactt
    14641 taatagatgc tttgagagaa aaaatagaaa aaacagaggt attaaatgct gagtttgttc
    14701 aagctatgct taaagaaata caaaaagagt acaaaataaa agggaaaaat ttatttatgg
    14761 gctcaagaat aattttgaca gggcaaatgc atggtccaga tttaccaaaa gtaatggaag
    14821 ttttaggaaa agaaacttgc ttaaatagaa tagcttatgt aaaaaataat atattatagt
    14881 cataaaagtc cgctaattta gaagcggact ttttttgtgt tagtatttgg tgaaaatatc
    14941 tgcttaagaa aatgaaggta tttaaggtaa taaatttaat atataaaaag caaagcaaaa
    15001 atataaataa aatatgaata ataaactaat aaaatattaa gcatagaaga agggaatcta
    15061 ttattttagt ttgatttaat tattaagggg gtaattacta tgtatagtaa tattgatgat
    15121 gttaaaaagg aattaaagga gctatgctta gaatatgtta caattttaga aaaattaaaa
    15181 gatgaaaaaa tgattacaga agaaactttt gaaaagtgct cttctcaaaa aaaaatattt
    15241 ttagaagaac aatagaggat ggtaatagta ttattattat gtagtttgaa agatagtacg
    15301 aaaaatgata gtaatactat tatttattgc aattttcaac tataatataa tatataatgt
    15361 taagaagata atttataaaa atacagtgag gtgatgagtg tgaaagttta taatacattg
    15421 actagaacaa aagaagaatt tgttccatta gaagaaggaa aggtaaaaat gtatgtgtgt
    15481 ggtccaacag tttataacta tatacacata ggtaatgcaa gaccatttat aatatttgat
    15541 actctaagaa ggtatttaga gtatagagga tatgatgtaa cttatgtaca aaactttaca
    15601 gatgttgatg ataagattat aaatagaagt catgaggagg gaatatctcc tgaagaagtt
    15661 gcggctaagt atataaaaga atattttgta gactgtgatg ggctaggaat aaaaagagct
    15721 actgttcatc ctcaggttac agataatata caacagatta tagagtttat aaaagagttg
    15781 gaagataagg gatatgcata tgcagtaaat ggtgatgtat attttgatac aaataagttt
    15841 gaaggttatg gaaaactttc aggtcaaaaa caagaagatt tagaggcagg ggctagaatt
    15901 gaggttaatg accaaaaaag acatcctatg gactttgttc tttggaaggc aaaaaaagaa
    15961 ggtgagccag gatgggatag cccttgggga gaaggtagac caggatggca tatagaatgt
    16021 tctgttatgt ctaagagata tttaggtgaa acaattgata tacatgcagg tgggcaagat
    16081 ttaacattcc cacatcatga aaatgaaata gctcaaagtg aagctagaag tggaaaaaca
    16141 ttctcaaaat actggatgca taatggatat ataaatataa atgatgaaaa gatgagtaaa
    16201 tcaaaaggta atttctttac agtaagagat atatcaaaat tatatgattt agagatagta
    16261 agatttttca tgttatcagc acattataga aatccagtaa actttagtga tgagatgtta
    16321 aatcaagcta aagcaggtct tgaaagacta tataatacta aagaaaaatt agaatttacg
    16381 ctaagtaatt tagtagaatc tcctttaaca gaaaaagaag tagagcttgt taaagaatta
    16441 gatgacttta gacaaaaatt catagatgct atggatgatg atgttaatac agcagacgca
    16501 gtaagtgtaa tatttgaatt agctaagtta ataaattcta atgtagatga aaattcttct
    16561 ttggagtttg ctaaaaaatg cttagatgaa tttaatgaac ttacaggagt attaaatatt
    16621 gtaaataaga aaaaagatac tgtattagat aaagacattg aagaattgat acaaaaaaga
    16681 actgatgcaa agaaaaataa agagtttcaa ttagctgatg atattagaca acaattacta
    16741 gatatgggaa ttgtattaga agacacaaga caaggtgtta agtggaaaag gatataaaaa
    16801 tggaaaaaac tgaattagtt actatgtcac cattagtttt agcatactta ggtgatacag
    16861 tgtatgagac atatataaga gaatatttaa ttagacagaa tacacaaaga aaagttaatg
    16921 atttgcataa actagctata aagtatgtaa aagctaaagc tcaggcaact ataattcatg
    16981 aaattgaaat tgagttaact gaagaagaga gtaaaattta taaaagggga agaaatcaaa
    17041 agtcaaatac ttctcctaag aatgcagata ttatagacta caaacatgca actgggtttg
    17101 aggcattagt gggatatttg tatttaaata atgaaataga gagacttcaa tatattatca
    17161 ataaaggaat caaaattatt gaaagagata tgtaaatatt aaagagtgtt gtcttaataa
    17221 taagtaataa ggcaacactt tttttataac taatgtataa taaatatata gaaattagta
    17281 atgagaaagg ataaaaagtt atgaaagtaa aattaatatc acatacacct gaaccagaaa
    17341 aagtaatagc tatggcagct aagctatgtt attcaccagt tgggacagac gaaatagaaa
    17401 aagacttaac agatgaaagt atagagaaat ttttaaatat gttattaagt ataggtcatg
    17461 ggtcaatatt agaacatgct tcatttacat tttcaataga aggtatttca agggcttgtt
    17521 cacatcaaat tgttagacat cgtatagcta gtttttcaca acaaagtcaa agatatgtta
    17581 aattagaaca atttgagtac ataattcctc cagaaataga aaaaatagaa aaggcaaaag
    17641 agttatttat agactccatg aaaaaagacc aagagaatta tgataagtta gttgaaatat
    17701 tgtttgaaaa tcattataat gacctaataa aaaacggtaa aaatgagaaa acagctaaaa
    17761 gacaagctga aaagaaagca atagaagatg ctagatacgt atttccaaat gcatgtgaaa
    17821 caaaaatggt atttactata aatgctagaa gtttgtttaa tttctttgaa catagatgct
    17881 gtgaaagagc acagtgggaa ataagaaatt tagctgtgga gatgttaaga gaggttaaaa
    17941 aggttgctcc tatattattt aaaaagaccg gtccaagttg tgtaaatgga agttgtccag
    18001 aaggaactat gacttgtgga gatatagttc aagttagaga aaaatttaag gccttgtagg
    18061 aggtgaataa tttggcgagt atagaaggaa gaaatccggt aatagaagca ataaaaagcg
    18121 acagagaaat agataaaata ttaattgcaa attcagctaa agaaggttca attaagaaaa
    18181 taataggaat ggctaaagat aagaatataa ttattcagta tgttgataaa cataagctag
    18241 atgaagtaag tacaagtcat tcacatcaag gtgtaatagc ttatgcaagt gagtataagt
    18301 attatgaatt agatgagcta atagatttag caaagaataa agatgaagac ccatttttca
    18361 ttatattaga tgagataaca gacccacata atttgggttc aataataagg acagcagatg
    18421 cagtaggagc acatggagtt atcattccaa aaagaaggtc tgtacacata actcctgtag
    18481 ttgccaaagc atctgctgga gcagtagaat atatgccagt ttgcaaggta accaatatag
    18541 ttaatacaat aaaaagactc aaagaagaag ggctgtggat tgctgcagct gatatggatg
    18601 gagaaacttt ttataaacaa aatcttactg gtcctcttgg tgtagtaatt ggaagtgaag
    18661 gttttggaat atctagattg gttaaacaaa actgtgactt tatagtaaaa atgccaatga
    18721 taggtaatgt tacatcttta aatgcatctg tagcaggtgg aatactttta tatgagatat
    18781 tcagacaaag actggataaa agtaaatagg catgaaaaaa aatataaatc attacttaat
    18841 aatagatggc tacaatatta taaatgcgtg ggacaatttg aaagaactgg ctaaagagga
    18901 cttagaagat tctcgagaaa agcttattga tgacattata gaattttctg agtttatggg
    18961 ttataagact ataatagttt ttgatgctta taatgttaaa aattctcgag aaaaagttga
    19021 aaaaagaaaa cacataacaa tagtttatac aagagaacac caaacagcag atagttatat
    19081 agagaaattt ataacttctt tatcaaagta tgatgatgta aaagtagcca ctaatgacta
    19141 tgcagaacag caaatgattt taggaaaagg tgctactaga atgtctgcaa gagaattaaa
    19201 gttagagtta gatcgttcca agaataaaat gaaggaaaaa aatatcggtt tgaggaaaaa
    19261 aattcagcga aattggttgg aggaaaggtt agataaagaa acattgtcga aacttgagaa
    19321 cattcgtaga aagcgttgaa attactaggt tctttagact ataatatacc tataaatttg
    19381 ttttatatat gggggagatt tacatgttgg tagcaaaaga aaaaagttat gagttagtgg
    19441 acaattgtca gcaggatgag tataacattg tattaagagc aagtgaaggg gataaaatag
    19501 cactagagta cattattact aaatacagaa attttgtaaa ggcaaaagca aagtcatatt
    19561 ttttaattgg agcagataaa gaagatataa tacaagaagg aatgataggc ctttataagg
    19621 cagttagaga ttttgatgga agtaagacca attcatttaa atgttttgcg gagatatgta
    19681 taacaagaca aataataact gctataaaaa ctgcaacaag acaaaaacat atacctttaa
    19741 attcatatgt ttctttgaat aaaccaatat atgatgaaga gtcagacaga acacttttag
    19801 atattatagc tacaagtata gtaacagatc cagaagagct tataattagt aaagaagagt
    19861 taaaaaatat agaatcaaaa atgaacgaat tgttaagtga cttggaatta gaagtactag
    19921 aattatattt aaatggaaag tcataccagt ttatagcgga caagcttaaa agagatgtaa
    19981 agtcaataga taacgcttta caaagggtta agagaaaatt agaaaaacat cttgaaaaca
    20041 gaaatgatta atagtaaaat acttaccata agcttattgt gagcaaaatt aatttatatc
    20101 gaaagataag ggaggaaatt caaaatggct aaagctaaat acgaaagaac aaaacctcat
    20161 gttaatatag ggacaatagg acacgtagac cacggtaaaa ctacattaac agcagcaata
    20221 acaaaaacat tatatgacag atatcaatta ggagaagcag tagatttcgc aaacatagat
    20281 aaagctccag aagaaagaga aagaggaatc acaatatcaa cagcacacgt tgagtatgaa
    20341 acaccaaata gacactatgc acacgttgac tgcccaggac atgctgacta cgttaagaac
    20401 atgataacag gagcagcaca aatggatgga gcaatattag tttgttcagc aacagatgga
    20461 ccaatgccac aaacaagaga gcatatacta ttatcaagac aagttggagt accatatata
    20521 gtagtattct taaacaaatg tgacatggta gatgatgaag agttattaga gttagtagag
    20581 atggaagtaa gagatttatt aacagaatat gatttcccag gagatgacac tccaatagta
    20641 agaggttcag cattaatggc attagaagat ccaaagagtg agtggggaga taagatagta
    20701 gaattattcg agcaaataga tgagtatata ccagctccag agagagatac agataaacca
    20761 ttcttaatgc cagtagagga cgtattctca atcacaggaa gaggaacagt tgcaacagga
    20821 agagtggaaa gaggagtact aaaagtacaa gacgaagtag aattagtagg attaacagaa
    20881 gcaccaagaa aagtagtagt aacaggagta gagatgttca gaaaattatt agaccaagca
    20941 caagcagggg ataatatagg agcattatta agaggagtac aaagaaacga gatagaaaga
    21001 ggacaagtac tagcaaagac tggatcagta aaggcacaca caaagtttac agcagaagta
    21061 tatgtactta aaaaagaaga gggtggaaga catacaccat tctttgatgg atatagacca
    21121 caattctatt tcagaacaac agacgtaaca ggagcttgta agttaccaga aggaatagag
    21181 atggtaatgc ctggagataa cgtaacaatg gaagtagact taataaactc aatagttgta
    21241 gaagagggat taagattctc aataagagaa ggtggaagaa cagtagcttc aggagttgtt
    21301 gctacaataa tagagtaatc ctttactaaa ctaatggatt taatataaaa aaagaggggg
    21361 gtaactcctc tcttttttgt tttttgttga caagtagcgc taaagcatat atactaaact
    21421 agtgccttga gacagattaa caatcaagtt taaggttatg atgttgtaat agaaaataaa
    21481 attgaaaaaa acagaaaaaa aacttgaaaa aatgtaacag atgatataaa atataatagt
    21541 gtgcttgaga gattaagaaa ataataatta aatcctagtg aaatacgaac tgatatcgat
    21601 ttaaaagtta caacacaccc ggagcagtgt atcggtattg gttgtacgca acttgaaaat
    21661 taaacacgtg ataaaggagg gaaaacaaaa tggctaagaa tgaaaaaata agaataagat
    21721 taaaatcata tgatcacaaa ttattagatt tttcagcagg taaaatagtt gaaactgcta
    21781 aaaaggctgg atcacaagtt tcaggacctg tgcctctacc aacagaaaaa caagttgtaa
    21841 ctatattaag agctgtacat aagtacaaag actctagaga acaatttgaa ataagaactc
    21901 ataaaagact aatcgacata gctaatccaa cacctaagac agttgactca ttaatgagat
    21961 tagacttacc agcaggtgta gatatagaaa taaaattata agaaccccta agatattatc
    22021 ctatagatga ttatcttaga atgattactc taaaagatgt aatccgctgt aagaattata
    22081 ggaggtgcta atatgaaagg aatattaggc aaaaaagttg gtatgactca aatattcact
    22141 gataaaggtg tagttatacc tgtaacagct gttgaagcag ggcctatggt cgttactcaa
    22201 ataaaaacag ttgataaaga tggatacaat gctatacaaa taggttttga agatgctaaa
    22261 gaaaaagctt taaacaaacc taaaaaagga catttagctg ctgctaatgt tttaaagaaa
    22321 catttaaaag aatttagagt agattctgta gaaggataca cagtaggaca agaaataaaa
    22381 gctgatgtat ttgaagcagg tgcaaaaata gatgttactg gaataagtaa aggtaaggga
    22441 ttccaaggtc caataaaaag acatggacaa agtagaggtc ctgaaactca cggttctaga
    22501 taccacagaa gaccaggttc aatgggagct tgttcttacc caggtagagt atttaaaaac
    22561 aaaaaattag ctggacatat gggaagcgta aaggtaacag ttcaaaactt agaggttgtt
    22621 aaagtagatg ctgacaagaa ccttatatta gttaaaggag ctataccagg agctaaaggt
    22681 tcagtagtaa ctataaaaga agctataaag gtttctaaat aatgactaac ctagagaaag
    22741 gaggaataac aatgccaaaa ttaaatgtac taaatgttag tggacaaaac gttggagaaa
    22801 tagaattatc agattctata tttggcgtag aagttaatgg acatgtttta tacgaagttg
    22861 taaaaaatca attagcaaat aagagacaag gaactcaatc tgctaaaact agagcagaag
    22921 ttagaggtgg cggaagaaaa ccttggaaac aaaaaggaac aggtagagca agacaaggtt
    22981 ctacaagatc tgtacaatgg gtaggtggag gagttgcttt tgcacctaaa ccaagaagct
    23041 acaaatatac attacctaag aaggtaagaa gattagctat gaagagtgct ttatcttcaa
    23101 aagttcaaaa tagtgaagtt atagtattag atgcattaaa catggatgct cctaagacta
    23161 aagaatttgc tcaaatatta aacaatataa atgctgctaa aaaagcttta gtagttatag
    23221 ctgacaaaaa cgataacgta ataaaatcag ctagaaatat agaaggtgtt caaactgcat
    23281 tagttaatac tatgaatgta tatgatatat taaaatacga ttcatttata ataactacag
    23341 atgctgttaa aaaagtggag gaggtgtacg cataatgact aatccacatg atgtaataat
    23401 aagaccagtt gtaactgagc acagtatggc tgaaatgggt gaaaaaaaat acacttttgt
    23461 tgtggctaaa gatgcgaaca aaactgaaat aaaaaaagca gtagaaaaag tattcggagt
    23521 tagtgttgat aaagtaaata ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac
    23581 tcaaggaaga acatcaagct ttaagaaagc agtagttaaa ttaactgctg atagtaaaga
    23641 aatagaattc ttccaaggaa tgtagtatta taacaaacga aggagggaaa acagatggct
    23701 ataaaaaagt ttagaccaac ttctcctgcc ttaagacaaa tgacagtttt agtttctgat
    23761 gaaataacat gtaatcaacc agaaaaatct cttttagtta acttaaagaa aaatgctggt
    23821 agaaacgtac atggtagaat aactgttcgt catagaggtg gaggacaaaa aagaaaatat
    23881 agaataatag attttaaaag agataaagat ggtatacctg ctaaggttgc tactatagaa
    23941 tatgatccaa acagaactgc aaatatcgca ttattaaact atgctgatgg agaaaaaaga
    24001 tatatattag ctccagttgg aattaatgtt ggagatacta tattatcagg attaggtgct
    24061 gatataaaac caggaaactg tttagcatta aaagatatgc cagttggtac tatcattcat
    24121 aatatagaat taaagccagg aaaaggagct caattagtta gatctgctgg agtttctgca
    24181 caattaatgg ctaaagaagg aaaaaatgct ttattaagat taccatcagg tgagatgaga
    24241 ttagtaagca taaactgtaa agctactata ggtcaagttg gaaatataga gcatggtaac
    24301 gtagttatcg gtaaagcagg tagaaaaaga catatgggaa taagacctac tgtaagaggt
    24361 tctgtaatga accctaatga ccatccacac ggtggagggg aaggtagatc tccaataggt
    24421 agaccatctc ctgttactcc atggggtaaa ccagctcttg gatacaaaac tagaaagaaa
    24481 aataaagctt caaataaact aatagtatca agaagaacta agtagtaaat ttatttttgc
    24541 tcggaaagga ggaatataaa tgtcaagatc aactaaaaaa gggccttttg tccatgcaag
    24601 acttttaaag aagatagaag ctatgaatgc tagtggaaat aaagaagtta taaaaacttg
    24661 gtcaagatct tcaactgtat ttccacaaat ggtagaaaac actatagctg ttcatgatgg
    24721 aagaaaacat gttccagtat atataacaga agatatggtt ggacataaat taggtgagtt
    24781 cgttcctact agaactttca aaggacataa ggatgacgaa aaatctaata agagaaaata
    24841 attaaattat gactaagaaa ggaggaataa caatggaagc aaaagctact gctaaatacg
    24901 tacgtgtatc accaagaaaa gcaggccaaa tatgtgacct tgttagagga aagaatgttg
    24961 atgaagcatt agcaatatta aagtttactc caagaggagc agcttcaata atagctaagg
    25021 ttgtaaaatc agctaaagct aacgcagaaa acaatcacga aatggatact gaaaaattat
    25081 atatagcatc aatagttgct aatcaaggac caacaatgaa gagattcatg cctagagcta
    25141 tgggtcgtgc aactacaata agaaaaagaa cttctcatat agaggttgtt gttaaagaaa
    25201 aaaaataatt aatagatagg agggaaagta atgggtcaaa aggttaatcc acacggctta
    25261 agggtcggtg ttataaaaga ttgggactca agatggttcg ctactgataa aaaagagttc
    25321 ggaaacttat tattagaaga ccataatata cgtaaattct taaagaaaag attatactca
    25381 gctggagttg ctaagataga aatagaaaga tcagctaaca aaataaaaat ggacttacac
    25441 gttgctaagc ctggtgtagt tataggaaga gctggtgcag gaatcgaagc attaaaagct
    25501 gaattagaaa aaatgacaaa gaaaactata atagttaata tagtagaagt aagaagtaca
    25561 gataaaaatg ctcaattagt agctgaaaat atagctttag ctatagagag aagggttgct
    25621 ttcagaagag caatgaaaca agctatacaa agagcaatga aatctggtgc taaagggata
    25681 aaagtttctg cttcaggaag attaggtgga gctgaaatgg ctagaactga aggttatagt
    25741 gaaggaaatg tgccactaca aactttaaga gcagatatag attatggttt cgctgaagct
    25801 gatacaactt atggaaaaat cggtataaaa gtttggatat gtaatggtga agttttacca
    25861 actagagacg gtgtaaatcc aagagaagaa agcagaaaga gcgatagaag agacaacaag
    25921 agagataata gaagaaacga tagaagagga aatgacagaa gaggaaatga taacagagga
    25981 aactacagag gacaaagacc tcaaggtgga tcaagacctc aaagaactga aaacaaagga
    26041 aattaataat agttaggttc aaggaaggag gaaaaatact catgttaatg ccaaaaagag
    26101 taaagcgtcg tagagttcat agaggaagca tggctgggca agctcataag ggtaataaag
    26161 ttacttatgg tgagtttgga ttagtagcat tagaagcttc ttggataact tctaatcaaa
    26221 tagaagctgc cagaatcgcg atgactagat atataaaaag agggggaaag gtttggataa
    26281 aaatattccc tcataaacca gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga
    26341 aaggttctcc agaatattgg gtagctgtag ttaagccagg aagagttatg tttgaattag
    26401 caggtgtttc tgaagataaa gcaagagaag ctatgagact tgctgcacat aaattaccaa
    26461 tcaaatgtaa atttgttaaa aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa
    26521 gctaaagaat taagagattt aacaagcgaa gagctaatga acaaattaaa cgacttcaaa
    26581 agtgaattat ttagcttaag attccaatta gctactggtc aattagaaaa tacagctaga
    26641 ataaagtttg ttaagaagga tatagcaaaa gttaaaactg ttcttgctga aagaaagtta
    26701 tacgaaacta gagcttaatt tggaaaggag gctttttaaa catggaaaga ggaagaagaa
    26761 aagttagaat aggccgtgtt gttagtgata agatggataa aacaatagta gtagctgttg
    26821 aagaattcgt acgtcatcca ctatataata aacgtgttaa gagaactaag aaattcaagg
    26881 ctcatgatga aaagaatata tgtaacatcg gagatagagt taaaataatg gaaactagac
    26941 cattatctaa agataagaga ttcagactag ttgaagttgt tgagaaagtt aagtagtttt
    27001 tgaagaagga gggattacga tatgatacaa caagaatcac gtctaagagt tgctgataac
    27061 tcaggagcta aagaactttt atgtatacgt gttctaggcg gaagtaaaag aagatatggt
    27121 aacataggcg acgttatagt tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa
    27181 aaaggtaagg ttgttaaagc tgttatagta agaagcaaac aaggcgtaag acgtaatgac
    27241 ggaagctata tatcttttga tgaaaatgct gctgttataa taaaggacga taaaactcca
    27301 gtaggaactc gtatattcgg gcctgttgct agagagttaa gagacaatga attcatgaaa
    27361 atagtttctc ttgctccaga agtactataa ttaaggaggt gcaataggac atgatgcgtg
    27421 ttaaaaaagg tgacactgtt gtagttatag caggtaaaga taaaggtaaa aaaggttcag
    27481 ttttaaaggt atatcctaaa actagcaaag tgttagttga aggtgtaaat gtaataacta
    27541 aacatcaaaa accaagtgct atgaaccaac aaggtggaat aataaataaa gaagccccaa
    27601 tacacatatc taatgtaatg ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat
    27661 atgaagtaaa agatgggaac aaagtaagag tatcagcaaa gagcggaaaa gaattataat
    27721 aaatattgct tgaaaggagg gaccttaaat ggcttctaga ttacaagaaa aatacatgaa
    27781 agaagttgct cctgctttaa tggagaaatt tggatacaaa aacgtaatgg agatacctaa
    27841 gttaaataaa atagttatta acatgggtat aggtgacgct agagaaaatc caaaaggatt
    27901 agaaaaaggc gttgaagaat tagaaatgat atcaggacaa aagcctgtta taactaaagc
    27961 tagaaaatct gttgctaact tcaaattaag agaaggaatg ccaataggaa caaaagttac
    28021 attaagagct gacaaaatgt tctacttcat ggacaaatta gtttcagttt ctttaccaag
    28081 agttagagac tttagaggag ttaaccctaa tgcttttgat ggtagaggaa attatgcttt
    28141 aggagttaaa gaacaattaa tattccctga aatagaatat gataaaatag ataaagtaag
    28201 aggaatggat ataatatttg ttactacagc aaaaactgac gaagaagctc gtgaattatt
    28261 aaaattatta ggaatgccat tttctaagta agcaaaggag ggattcccgt ggctagaaaa
    28321 gcgatggttg ttaagcaaca aagaaagcaa aagtacgcta ctagagaata cactagatgt
    28381 actatatgcg gaagaccgca ttcagttttg aaaaaatttg gtatatgccg tatatgcttt
    28441 agagaattag cttataaagg tcaaatacct ggtgttagaa aagcaagctg gtagaagctt
    28501 gattagaatg gaaggagggc aacaaaatga caatgacaga tccaattgca gatatgctta
    28561 ctcgtataag aaatgctaat gttgttaagc atgaaactgt agatgttcca gcttctaata
    28621 tgaagaaaga attagctaga atcttattag aagaaggttt cataagaggt tatgatgtta
    28681 tagaagatgg aaaacaaggt atcataagaa tacaattaaa gtacggacaa gaaggcgaaa
    28741 gagttataac aggtcttaag aaaatatcta agcctggaat gagagtttac gctgctaacc
    28801 atgagatacc aaaagtatta aacggattag gaatatcagt tatatctact tcaaaaggaa
    28861 tattaactga caagcaagct agaaaagaaa atgttggtgg agaagtaatc tgctacgttt
    28921 ggtaattaat atgaacaatg taaggaggtg cgaccatgtc aagaataggt gttaagccaa
    28981 taataatacc agcaggtgtt gaagttacta tagctgaagg aaatttagtt acagtaaaag
    29041 gtccaaaagg aactttaact aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa
    29101 atacaataat ggttgaaaga ccaactgata ataaaaaaca tagatcttta cacggactaa
    29161 ctagaacttt attagataat atggttgtag gtgtaaatac tggtttcgaa aagaaattag
    29221 aattaaaagg tgttggatat agagctcaaa aacaaggaaa gaaattagtt atgaacttag
    29281 gtttctctca tccagttgag atggaagatc cagaaggaat aactgttgaa gctccaaacc
    29341 aaacagagtt aatagtaaaa ggtatagaca agcaattagt agggaattat gctgctaaga
    29401 taagagcttg gagaaagcca gagccataca aaggtaaagg tataaaatac gttgatgaag
    29461 taatcagacg taaagaagga aaaactggta aaaaataata ccaaaactta aacgctagaa
    29521 aggagtgagc taggtgttaa aaaaggctga taaaaatgct aatagacttc aaagacataa
    29581 gagagttcgt agaaaaataa gcggtacttc tcaaagacca agattatgtg tatttagaag
    29641 tgctaacaat atatatgctc aaataataga tgacacaaaa agagtaactc tagttgctgc
    29701 atcttcttta gaagcagaag ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc
    29761 taagaaagtt ggagaattag ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt
    29821 atttgacaga ggtggatact tatatcatgg aagaattcaa gaattagctg aaggtgctag
    29881 agaagctggt cttaagttct aatacgaagg agggaaaaat acatgctacg tcgtaagccg
    29941 atagatgcag gacaacttga cttacaagaa aaagttgttg aagtaagacg tgttactaag
    30001 gttgtaaaag gtggtagaaa tttcagattt gcagcattag tagttgttgg agatgaaaac
    30061 ggacacgttg gtataggtgc tgggaaagca atggaagtac cagacgctat aaaaaaagca
    30121 gttgaagatg ctaagaaaaa tctaatagta gtgccaatag ttgggactac aattcctcac
    30181 gaagtacgcg gacactttgg tgctggaaat atattaataa tgcctgctgt agaaggtaca
    30241 ggagtaatag ctgggggacc tgctagagcc gtacttgagc tagctggact aaaagacgtt
    30301 agagctaaat ctttagggtc taacaatcca agaaatatgg taaatgctac aatagaagga
    30361 cttaattctc taaaaacagt tgaagatata gctaaactta gaggtaaaaa agttgaagaa
    30421 cttctagggt aaggaggtag tgtaaaatgg ctaaattaca aataaagtta gttagaagtg
    30481 ttataggaac tactcctaac caaaaaaaga atgttgaagc gttaggatta agaaaaagag
    30541 aacaagttgt tgtaaaagaa gacaatgctc aaacaagagg tatgataaat aaagtaagtc
    30601 acttattaga agtaactgaa atagctgaat aatttttata aagtagtgca aggaggtgca
    30661 atccatgaag ttacatgagt taaaacctgc tgaaggtgca gtaagagcta agagaagatt
    30721 aggtagaggt actgcaactg gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg
    30781 gtctcgttct ggtggtggag taagagtagg atttgaaggt ggacaaatgc cactagctag
    30841 aagacttcct aagagaggtt tcaataacat atttaagaaa gtttacacag aagttaatgt
    30901 tgaagtttta aacagatttg aaaatggaac agaaataact gcagaattat taaaatctac
    30961 taaaactata agcaaaatag gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga
    31021 gaaagcttta actgttaaag ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa
    31081 agctggtgga aaagcagagt tagtataata acctgctaat ctcctagcca gtacaggcgg
    31141 ggtgaattag cgtgctgtca aaattaaaac aagcttggaa gattaaagca gtaagaaaaa
    31201 aagtaatgta tacacttatg atgatcgtga tattcagaat aggtactact ataccagttc
    31261 caggcataga cactagtatt atacaaaaga tggtaggcgg caatagtcta ctttctctgt
    31321 ataatatgtt tactggtgga gcatttagta acttttcact atttgcttta ggtataagtc
    31381 catatatcac tgcatcaatc ataatacaac ttctaacagt aggttttgaa agtttagcag
    31441 aacttcaaaa atctggtgaa gaaggaaaga aaaaaatcaa taagtacact aaatatacag
    31501 cactagctct agcagttgta caagctttgg gaattacatt aggaattgta agaagtgctt
    31561 taatatcaaa tagtgtgttt tttatcacaa ctgttgtaat aacattagtt tcagcaagta
    31621 tgttagtaat gtggattggg gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag
    31681 taataatatt tgcaggtata atatcaagaa tacctacaga tgttattaaa atatcgcaac
    31741 aagttaaatc tggcgaagtt gcaccttggg ttatagtgat tctagctgta gttatattac
    31801 ttacagtaac aggagttaca tttatacaag aagcaactag aaagatacct gtacaatatg
    31861 ctaaaagagt tgttggaaga aaaatgtatg gaggacaaag ttctcatata ccaatgaaag
    31921 ttaaccagtc aggagttatg cctataattt ttgctagttc acttttagct ttccctcaaa
    31981 caatagctat gtttatgggt ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg
    32041 caacagaaca aggattttgg acatatagat caattgagat tctacttata attttcttct
    32101 catattttta tacaactgta tcatttaata cagaagacat atctaaaaat atgaaaaata
    32161 atggtggatt tataccaggt ataagaccag gtgagcctac tatggattat ttaaatagaa
    32221 ttttaactag attaacttta gctggagcta ctttcttagc aataattgct atggtgccag
    32281 ctcttacaac acattatatg aaagtaaata tgagtctagc tgggacttca ttgctaatcg
    32341 ttgttggagt tgctttggag cttaagagac agttagagtc aaacttagtt atgagaagct
    32401 atcaaggttt cttaaaataa aatggagatg gataatatga gaataatatt acttggacct
    32461 ccaggtgctg gtaaaggtac tcaggcggca ggaatagtag aaaaatacaa tatacctcat
    32521 atatcaacag gagatatatt cagaaagaat ataaaagagg gaacagaact tggaaaaaaa
    32581 gctaaagaat acatggacca aggtttatta gtaccagatg agttaactgt aggtttagtt
    32641 actgatagaa tatctcaaga agattgtaaa aatggattta tgttagatgg atttccaaga
    32701 aatgtagcac aaggagaaca tttagatatc ttcttaaaaa atgctggtat atcactagat
    32761 aaagttgtca atattgaagt tgataagagt atattagtgt ctagagcagt tggtagaaga
    32821 atatgtaagt cttgtggagc tacttaccat gttgagttta atcctcctaa agtagaaggt
    32881 gtatgtgatg tatgccaagg agaattatat caaagagctg atgataatga agaaactgta
    32941 tctaagagaa tacaagttta tctagatgaa actaagcctt tagtagatta ttatagcaaa
    33001 caaggtataa tagcagatat aaaaggtgat caagcaatag ataaagtatt tgaagatatt
    33061 gtcgcagcct taggaagtgg aaaataaatg attatcttga aatcaaaaaa agaaatagaa
    33121 cttttaagag aagcaggtaa aatagttgct gacactcatg aagttttaag aaaagctatt
    33181 tctccgggaa tatctacttt agaattagat aaaatagctg aagagaatat aagaaaatac
    33241 aatgcagaac cttcttttaa aggatatgga ggttttccag gaagtatatg tgcttctatc
    33301 aatagagagg tagtacatgg tattccagga gaaactatat tacaagaagg tgacattgta
    33361 agtttggata taggagccta ttataaagga tatcatggtg attctgcgaa aactcacgga
    33421 gtaggcatga tatctgaaga agataggaaa ttgatagaag taactagaga aagcttctat
    33481 gaaggaataa aatttgctaa attaggatat agactttcag atatctcaca cgcagtacaa
    33541 acacacgttg agaaacatgg tttctcggta gttagagatt tagtgggtca tggagttgga
    33601 gcaaatcttc atgaagaccc acaagtacct aattatggac tcccaggaaa aggaccaaga
    33661 cttagagaag gaatggttat tgctatagag ccaatggtta atgcaggtcg ttatcatgta
    33721 aagactctat cagatgggtg gacgactgtt acaatagatg gaaaaaaatc agctcattat
    33781 gagcatacga tagcaattac tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa
    33841 aagtaggtga aaaaagtgct atcagataat ttaagtatag gtcaagttgt taaagtttca
    33901 ttaggacgag ataaaggaaa tttattcttt gtagtcaaaa taattaataa tgaatatgta
    33961 ttaatagctg atggaaaaaa gagaaaactt gacaaaccta agttaaagaa agtgaagcac
    34021 ttaaaaaaat ataattttat taatgatgaa gttagaaaaa gagtagtatc tggacaagag
    34081 attacagatt cttttttaag agctgaactt actaagttaa attagataat ttaacttatt
    34141 gaaatggagg gctggttagt taatggccaa aaaagatgtt atagaattag aaggtacagt
    34201 ttcagaagct ttacctaatg ctatgttcaa agttaaacta gaaaatggac atgagatatt
    34261 atgccacatt tctggaaagc taagaatgaa cttcataaga attcttgaag gtgacaaggt
    34321 gaatgttgaa ctttctccat atgaccttac aagaggaaga attacttggc gtaagaagta
    34381 gactatatta atatttagtc taaggaggga ttaataatga aggttagacc atcagtaaaa
    34441 ccaatatgtg aaaaatgtaa agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa
    34501 aatccaaagc ataagcaaaa gcaaggataa gaaattatta ctagcatttt gttaaatttt
    34561 atagtataat actatattgt gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg
    34621 cgtaggattg gcatatgatg tgaagtttta aacactaaaa actatgtgta ggaggtgcga
    34681 tcatggcaag aatagctggg gtagatttac ctagagaaaa aagagcggag ataggcttaa
    34741 cttatatata tggtataggt aaagcaactg ctaacgaaat attagctaag gctgagataa
    34801 atccagatac tagaataaaa gatttatcag aagatcaagt taatgagtta agaaaagtta
    34861 tagatgacga tttcttagtt gaaggtgact taagaagaga aattgcttta aacataaaga
    34921 gattaagaga tataaaatgt tatagaggaa taagacacgc taaaggtctt ccattaagag
    34981 gacaaagaac taagactaat gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta
    35041 aaaagaaaaa ataaataaat aataactaga tgaaggaggg aaaaggaaaa tggctaaacc
    35101 aaaaaagaaa gttacacgta ttagaagaag agaacgtaaa aatatagaac gtggtcatgc
    35161 tcatatacaa tcaactttca ataatacaat aataacttta actgacgttc acggaaatgc
    35221 tatatcttgg gcaagttctg gacaattagg attcaaagga tcaagaaaat caactccatt
    35281 tgcatctcaa atggctgctg aaacagctgc aaaagctgcg atggaacacg gactaaaaag
    35341 tgttgaggta ttcgtaaagg ggccaggttc aggaagagaa gctgcaataa gagctttaca
    35401 agcaactgga ctagaagtaa ctatgataaa agacgttact ccaatcccac ataacggatg
    35461 cagaccacca aagagaagaa gagtgtaatt aataagagta caataggagg tgtagataaa
    35521 tggcaagata tacaggtgca tcatgtagac aatgccgtag agagggaatg aaattattcc
    35581 ttaaaggtga tagatgttat acagacaaat gtgctatagt aaaaagaaac tatgctccag
    35641 gtcaacatgg ccaaggaaga aagaaagttt ctaactatgg attacaatta agagaaaaac
    35701 aaaaagttaa aagaatatat ggagttttag agactcaatt tagaaattta tatgaacgtg
    35761 ctgaaaatat gcctggtaaa gcaggtgaga acttattaag tttattagag agaagattag
    35821 ataatgtagt ttacagaatg ggactagcat cttctagaaa agaagctaga caattagtga
    35881 ctcatggtca tttcacttta aatggaaata aagtagacat accttcatta atagttaagg
    35941 ttggagatgt tatagaagtt aaagagaaat caagatcttc tgcaaaattc aaaaatttag
    36001 tagaagttaa ctcaagaata gctcctaagt ggttagaagc taatgtagaa ggaatgacag
    36061 caaaagtagt tggtgttcca acaagagaag atatagatct tgagatagca gagcacttaa
    36121 tcatagaact ttactctaaa taataagcac acttttaaaa attattttaa aattttgttt
    36181 accctcagtg gattactgaa ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc
    36241 aaaagtagat atagttgagc ttagcgaaga ctatagatat ggtaagtttg ttatagagcc
    36301 tctagaaaga ggatatggaa taactatagg taacgcatta agaagaatat tattatcatc
    36361 attaccaggt gtagcagtga atgctataaa aatagatgga gttcttcatg aattttcaac
    36421 aatacctggt gttaaagaag atgttactga gataatatta actttaaaag agctttcagc
    36481 aactatagat ggtgaaggaa gtagaacact taaaatagaa gctcaaggac catgctctat
    36541 cacaggtgca gatataatct gtcctccaga tgttgaaata ttaagtaaag atttagcaat
    36601 agctacatta gatgataatg ctaaacttaa tatggagata tttgtagata aaggtagagg
    36661 ttatgtttct gctgaagaaa ataaaacaga gaatgttcca ataggtgttt tacctgtgga
    36721 ttcaatatat actcctgttg agaaggttag ttatcatgtg gaaaacacaa gagttggtca
    36781 aaaaacagat tatgataaat tagtattaga agtttggact aatggtagta taaatcctca
    36841 agagggaata tcattagctg caaaggtatt agttgagcat ttaaacttat ttatagactt
    36901 aactgaacat gtaagtagtg ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa
    36961 agttcttgaa atgactatag aagaattaga tttatcagtt agatcatata actgtttaaa
    37021 gagagcggga attaatacag ttgaagaatt agctaataaa tctgaggatg atatgatgaa
    37081 ggttagaaac ctaggtaaga agtcattaga agaggtaata caaaaactag aagaacttgg
    37141 attgggatta aaaccaagtg aagaatagcg agcaaaggag ggataagaat ggctaagtac
    37201 cgtaaattag gacgtgaaac agctcacaga aaccttatgt taagaaactt agtaacttgc
    37261 ttactaagaa gtggaagaat agaaactaca gtgactagag cgaaagaaac tcgtagaatg
    37321 gctgaaaaga tgataactct tgctaagaga ggagatcttc atgctagaag acaagtttta
    37381 gcttatgtta tggatgaaac agtagttaat aacttattca cagatttagc tccaaaatat
    37441 gctgagagaa atggtggata cactagaata ataaaaatag ggccaagaaa aggcgatgct
    37501 gctgaaatgg cttttataga attagtatag taaggaaagg attaggcttg atggcttaat
    37561 ctttttttta tttttttgac taaggtatga tataataatg tgtaaaatga tttttataag
    37621 ttattgctta ctaaaaggag tatgagttaa tggataatat agtaaaggta aataatattt
    37681 cgtttgagta tattacagat gaagcaaaac ttaaagcaat agataattta agcctagatg
    37741 ttaaaaaagg agaatttgtt gcgataatag gacataatgg ttctggtaag tctactttat
    37801 ctaaaaactt aaatgctatt cttatgccta ctgaaggaaa tattcttatt gatgatatgg
    37861 atactaaaga agaagaaaga ttatgggata taagacaaac tgcaggcatg gtatttcaaa
    37921 atccagataa tcaaatagtt gctactatag tagaagaaga cgttgcattt ggacctgaga
    37981 acttaggtat agaaccaaaa gaaataagac gaattgtaga ggagtcttta aaaagtgtag
    38041 gtatgtatga tttaagagat agacagcctc atttgttatc aggaggtcaa aagcaaagag
    38101 ttgcaatagc aggaattatt gctatgaggc ctaaatgtat aatatttgat gaagcaacag
    38161 caatgttaga tccatctgga agaaaagaag ttatgaaaac aataaaaaga cttaataagg
    38221 aagaaaatat aactgttata catataacgc actttatgga agaagcagta gaagctgata
    38281 gagttgtagt tatggaaaaa ggaaagaaaa tcttagaggg aactcctaga gaagtattca
    38341 gtaaaataaa gatgttaaag gaaatcggtt tagatgtacc ttgtatgaca gagttatcta
    38401 gtttattaat agaagaaggt ataaatatta gtagtgatat attaactgtg gatgagatgg
    38461 tgatggaatt atgtcaatta tagtaaagaa tttaacacat atatataatg aagggatgcc
    38521 ttttgcaagt aaggcacttg atgatgtttc ttttgagatt aaagatagag attttgttgg
    38581 tcttataggt catacaggct caggaaaatc tacattaata caacatttaa atggattact
    38641 aaagccttcc tcaggagaaa tatttataaa tgattttaat ataacagata aaaacttgaa
    38701 tttaactgag attagaaaaa gagtaggggt tgtatttcaa tatccagaat accaattgtt
    38761 tgaagaaacg attgataaag atatagcttt tggaccctct aatttaggct tagaggagtc
    38821 tgagatacat aacagggtaa aagcatcaat ggaagctgta ggacttgatt atgagggatt
    38881 taaagacaaa tctccttttg aattatctgg gggacaaaag cgtagagtag caattgctgg
    38941 agttatagcc atgaacccag aagttctcat attggatgaa ccaactgctg gtcttgaccc
    39001 tggtggaaga gatgaaattt ttaacttaat aaaagactta catgaaaaaa agaatatgac
    39061 tataatacta tcatcacata gtatggatga tatggcaaag ttagcaaaaa ctttaattgt
    39121 tatgaatcat ggtagcgtag aatttatggg aactcctaga gaagtgttta aatcaaatgc
    39181 tagtaaatta aaagatatag gattagatat tccacaagtt ttagaacttg cattgaaatt
    39241 gagagaaaaa ggctttgaca tcagtgagga tatattgact ttagaagaag caaaacagga
    39301 aatattaaaa gttgtgagag gacgaggatt atgttaaaag atataactat agggcaatac
    39361 tacccaacaa gttctgctat tcataaatta gatccaagga taaagcttgt agcgacaatt
    39421 gtatttatgg tatctatatt tgtagtaaat aaattttggc cttatatagt agttttatta
    39481 tgtttattag ctatgataaa attggctaat ataccagtca aatatattgt taagggtgtt
    39541 aaacctctta aatggattat tttgtttaca tttcttataa atattttttt cttaccaggt
    39601 gatgaaatat ggtcatttgg atttttagct attacaaaac agggtctaag acaagcaatt
    39661 tttatggcaa taagattaat atttttagta gttggtacat cattgcttac tttaacaact
    39721 tcaccaatag aattaactga tggaatagag agattgttga atccatttaa gaaaattggt
    39781 ttgccagttc atgaacttgc aatgatgatg acaattgctt tacgttttat tcctacattg
    39841 ttagatgaga cagacaaaat aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt
    39901 aagaatctta taaatagagc aaagaattta gtaccacttt tggtaccttt atttgtaagt
    39961 gcatttagaa gagctgatga attggctatg gccatggagg ccagatgtta cagaggtgga
    40021 cataatagga ctaagatgag agagtctgtt atatcaagaa gggattatat ggcatgtgta
    40081 tttcaggttg tttatctggg agcaattata gctactagat ttatagcaat ttaattaata
    40141 agttatagat ataatgaggt aaagtatgag aaatataaaa attaaaatcc aatacaatgg
    40201 taagaattat tgtggatggc aaaagcaacc tgattcttta gggatacaag gtacaattga
    40261 aagggctata tatgatataa caaaagaaga aaccagtctt atagggtctg gtagaactga
    40321 ttcaggagtt catgctattg gtcagattgc aaattttaaa attaattcag gtataagtat
    40381 agaaagtata ccaatggcac taaatgccaa gttgccaaaa gacatatcag ttatagaagc
    40441 atgtgaagta aatgatgatt ttcactccag atacagtgca aaaggtaaaa catataaata
    40501 tttagtatat aatagcaagt ttagaaatcc aatacttagt gagatatcat atcaagtaaa
    40561 atatgaactt gattttgata aaatgtgttc agaagcaaaa agtttattag gaacacatga
    40621 ttttaaaggt tttatgagtt ctggttcatc tgtaaaagat acagttagaa cgatatatga
    40681 tattgatata agtaaaaaag atgacttgat tacttttgag attagtggta atggtttttt
    40741 atataacatg gtgaggatta ttgttggaac acttgttgat atgggtcgtg gcagaattaa
    40801 cgaaccattt ttagatataa tacaatcaaa aactaggtca agatgtggac atacagctcc
    40861 agcacaagga ttatttctca aaaaagttca ttattaactt gacacaccag gctacatgta
    40921 ttaaaatata tgagaatgtg ttaataagtc cacttgcccc ggacttgaca taaacggtaa
    40981 aaaaatttta tgaaaagtag ttaaggaggg acagttatga aaagttatat agctaagcca
    41041 gctgatgtac aaagaaaatg gtacttagtt gatgctgaag gaaaaacatt aggtcgttta
    41101 gcaacagaaa ttgcgacagt attgagaggt aaacataaac caacatttac tcctcacgtt
    41161 gatggtggag attttgttgt tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa
    41221 ttagatcaaa aatattacag ataccatact ggttatgtag gtggattaaa agaaatatct
    41281 tacagagata tgatggataa gaaaccagaa gaagttatat cacatgctgt aagcggtatg
    41341 ttacctaaaa ataaattaag aagcagaatg atgactagat taagagtatt tgcaggtgct
    41401 gagcacactc atgcagctca aaatccagaa gttttaaact ttaaataaga ttaataggtg
    41461 aaaggaggag tttataatgg ctaacgttca atattatgga actggaagaa gaaaaagttc
    41521 tgttgctaga gtaagactag ttgcaggtga aggaaatata ttagtaaatg gaagagcatt
    41581 agaaaattat tttaattatg agacattaat aagagatgtt aaacaaccat tagttttaac
    41641 tggtaatgaa aataaatatg atgttatagt aaaagttgaa ggtggaggat tcactggaca
    41701 agctggagct ataagacatg gaatatctag agctttatta aaagctgatt tagatttaag
    41761 acctgcttta aagaaagaag gtttcttaac tagagatgca agaatgaagg aaagaaagaa
    41821 atacggatta aaagcagcaa gaagagctcc acaattctca aaaagataat tacttatttt
    41881 tttggaaaag caaaatcctt tatttactgt tattcagaaa gtattggtat tttaaaaatg
    41941 ttgggttaag ttaattattg atatataata aaataataag atatcttatt aggatgtttt
    42001 tcattaagaa gaacatcctt tttattttga ctatttaata aaaaacatat gctaat
//
LOCUS       DACULQ010000010        41765 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.010, whole genome shotgun sequence.
ACCESSION   DACULQ010000010 DACULQ010000000
VERSION     DACULQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.010"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            432..569
                     /locus_tag="JA154_02340"
     CDS             432..569
                     /locus_tag="JA154_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAT7994698.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            647..991
                     /locus_tag="JA154_02345"
     CDS             647..991
                     /locus_tag="JA154_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAT7994699.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            1023..1274
                     /locus_tag="JA154_02350"
     CDS             1023..1274
                     /locus_tag="JA154_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAT7994700.1"
                     /translation="MNKIIYILKEISKHLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            1306..2013
                     /locus_tag="JA154_02355"
     CDS             1306..2013
                     /locus_tag="JA154_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HAT7994701.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            1982..2611
                     /locus_tag="JA154_02360"
     CDS             1982..2611
                     /locus_tag="JA154_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAT7994702.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            2723..4102
                     /locus_tag="JA154_02365"
     CDS             2723..4102
                     /locus_tag="JA154_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAT7994703.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENVIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            4197..6092
                     /locus_tag="JA154_02370"
     CDS             4197..6092
                     /locus_tag="JA154_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAT7994704.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            6085..6804
                     /locus_tag="JA154_02375"
     CDS             6085..6804
                     /locus_tag="JA154_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAT7994705.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            6967..7752
                     /locus_tag="JA154_02380"
     CDS             6967..7752
                     /locus_tag="JA154_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HAT7994706.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            7887..8660
                     /locus_tag="JA154_02385"
     CDS             7887..8660
                     /locus_tag="JA154_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAT7994707.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            8662..9525
                     /locus_tag="JA154_02390"
     CDS             8662..9525
                     /locus_tag="JA154_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HAT7994708.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            9558..10700
                     /locus_tag="JA154_02395"
     CDS             9558..10700
                     /locus_tag="JA154_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAT7994709.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(10834..11409)
                     /locus_tag="JA154_02400"
     CDS             complement(10834..11409)
                     /locus_tag="JA154_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994710.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            11669..12961
                     /locus_tag="JA154_02405"
     CDS             11669..12961
                     /locus_tag="JA154_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7994711.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSESKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            13066..13668
                     /locus_tag="JA154_02410"
     CDS             13066..13668
                     /locus_tag="JA154_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAT7994712.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            13741..14004
                     /locus_tag="JA154_02415"
     CDS             13741..14004
                     /locus_tag="JA154_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HAT7994713.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            14019..14204
                     /locus_tag="JA154_02420"
     CDS             14019..14204
                     /locus_tag="JA154_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAT7994714.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            14307..15503
                     /locus_tag="JA154_02425"
     CDS             14307..15503
                     /locus_tag="JA154_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAT7994715.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            15717..15995
                     /locus_tag="JA154_02430"
     CDS             15717..15995
                     /locus_tag="JA154_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAT7994716.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            16032..16466
                     /locus_tag="JA154_02435"
     CDS             16032..16466
                     /locus_tag="JA154_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAT7994717.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            16485..16712
                     /locus_tag="JA154_02440"
     CDS             16485..16712
                     /locus_tag="JA154_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAT7994718.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            16855..17175
                     /locus_tag="JA154_02445"
     CDS             16855..17175
                     /locus_tag="JA154_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994719.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            17183..18139
                     /locus_tag="JA154_02450"
     CDS             17183..18139
                     /locus_tag="JA154_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HAT7994720.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            18150..20144
                     /locus_tag="JA154_02455"
     CDS             18150..20144
                     /locus_tag="JA154_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAT7994721.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            20145..20594
                     /locus_tag="JA154_02460"
     CDS             20145..20594
                     /locus_tag="JA154_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAT7994722.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            20613..21941
                     /locus_tag="JA154_02465"
     CDS             20613..21941
                     /locus_tag="JA154_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090181.1"
                     /note="unwinds double stranded DNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAT7994723.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            22146..22454
                     /locus_tag="JA154_02470"
     CDS             22146..22454
                     /locus_tag="JA154_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994724.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            22536..23825
                     /locus_tag="JA154_02475"
     CDS             22536..23825
                     /locus_tag="JA154_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAT7994725.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            24151..24226
                     /locus_tag="JA154_02480"
     tRNA            24151..24226
                     /locus_tag="JA154_02480"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24184..24186,aa:Lys,seq:ttt)
     gene            24233..24307
                     /locus_tag="JA154_02485"
     tRNA            24233..24307
                     /locus_tag="JA154_02485"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24266..24268,aa:Glu,seq:ttc)
     gene            24312..24387
                     /locus_tag="JA154_02490"
     tRNA            24312..24387
                     /locus_tag="JA154_02490"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24345..24347,aa:Val,seq:tac)
     gene            24421..24495
                     /locus_tag="JA154_02495"
     tRNA            24421..24495
                     /locus_tag="JA154_02495"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24453..24455,aa:Thr,seq:tgt)
     gene            complement(24878..25870)
                     /locus_tag="JA154_02500"
     CDS             complement(24878..25870)
                     /locus_tag="JA154_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAT7994726.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(25879..26937)
                     /locus_tag="JA154_02505"
     CDS             complement(25879..26937)
                     /locus_tag="JA154_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HAT7994727.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            27119..28525
                     /locus_tag="JA154_02510"
     CDS             27119..28525
                     /locus_tag="JA154_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HAT7994728.1"
                     /translation="MVLIIGCFIEKDDSLETYKEKTNSKINQYNMDVIFDDETKRLMC
                     NQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEMTRAYPNGFNEGYIDIKNIL
                     NNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYNVKIPNSIGRFGYGENTINV
                     TNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLLIPNKYKIAHTGKLIDDKHD
                     NKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTYNLNKKLSKKATEVAKDSIN
                     IFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLYNEKNEFLLEYVIAHETAHQ
                     WWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMANKLIKTMEIQTKSYLSENIF
                     KPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFETLNEYYNKYKHKNANGRAFV
                     ELWNSKGVDINKIISECK"
     gene            28627..29424
                     /locus_tag="JA154_02515"
     CDS             28627..29424
                     /locus_tag="JA154_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT7994729.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            29437..31539
                     /locus_tag="JA154_02520"
     CDS             29437..31539
                     /locus_tag="JA154_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HAT7994730.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            31607..32086
                     /locus_tag="JA154_02525"
     CDS             31607..32086
                     /locus_tag="JA154_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAT7994731.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            32235..32546
                     /locus_tag="JA154_02530"
     CDS             32235..32546
                     /locus_tag="JA154_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT7994732.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            32594..32911
                     /locus_tag="JA154_02535"
     CDS             32594..32911
                     /locus_tag="JA154_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAT7994733.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            33124..33825
                     /locus_tag="JA154_02540"
     CDS             33124..33825
                     /locus_tag="JA154_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7994734.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            34049..35350
                     /locus_tag="JA154_02545"
     CDS             34049..35350
                     /locus_tag="JA154_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT7994735.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            35487..36581
                     /locus_tag="JA154_02550"
     CDS             35487..36581
                     /locus_tag="JA154_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAT7994736.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIMLLDFIEPWSKFKLILEGE"
     gene            36583..37716
                     /locus_tag="JA154_02555"
     CDS             36583..37716
                     /locus_tag="JA154_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAT7994737.1"
                     /translation="MKIDILIKGGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            37734..38843
                     /locus_tag="JA154_02560"
     CDS             37734..38843
                     /locus_tag="JA154_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelA-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HAT7994738.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            38908..39648
                     /locus_tag="JA154_02565"
     CDS             38908..39648
                     /locus_tag="JA154_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphooctonate aldolase"
                     /protein_id="HAT7994739.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGYVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            39653..40645
                     /locus_tag="JA154_02570"
     CDS             39653..40645
                     /locus_tag="JA154_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAT7994740.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVLICGIAMQLTDSIKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(40833..41714)
                     /locus_tag="JA154_02575"
     CDS             complement(40833..41714)
                     /locus_tag="JA154_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT7994741.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
BASE COUNT        16654 a         4464 c         7468 g        13179 t
ORIGIN      
        1 agtaatctta aaagttatac acaaaagtat ccacaagctg tgaataattt ctaataagtt
       61 gataacattt atgcacatta tcaacaagta tatttataat atcaaaatat atgtcaaact
      121 tcaatgattt gtaactaatt tatccacaat attaactaat tgttgataaa aatataaaca
      181 tgttaaaatt actttatttg tgtatataat tgaaaaaaat aatttagaat aaaattaaat
      241 agatttttac tagtattttc aataaaaaaa atacataata tatatatggt agattttgtg
      301 atataagcgt cccaaaaact cgttgacaac agtagtaaaa gtatttataa ttatatgtgt
      361 tatgtttact ttgagggtaa agttattatt tttataaacg gaaaataaaa tctgaaaggc
      421 ggtgcaaaat aatgagtaaa agaacttatc aaccaaaaaa gagacaaaga agtaaagaac
      481 atggtttcag aaaaagaatg aagacgtcta acggaagaaa cgtgttaaag agaagaagag
      541 ctaaaggaag aaatagatta actcattaat tacaaaaggg ccgctgcaaa ggtggccttt
      601 ttgtgtaaat aattacaatt catagctgtg tttatggagg ttaactatgg actttaatag
      661 gactaaaggg ttgaaaaaag actctgattt tagaaaagta tataaacacg gcaaatcttt
      721 tgcaaacaaa tatttagtaa tatatatact taaaaataaa tcagattata gtagagtagg
      781 tatttctgtt tcaaaaaaag taggaaaagc tattactagg aatagagtaa gaagattaat
      841 aaaagaagcc tacagattaa atattgatga aaaaattaag cctggatatg atatagtatt
      901 tatagcaagg gtatctagta aggatgctac ttttaaagac atagacaagt ctataaaaaa
      961 tttagttaaa agaacggata tttctattta attgtatgct ataaatatgg aggttaatga
     1021 ctttgaataa aataatttat atattaaagg aaataagtaa gcatttatcg aatttatgta
     1081 tttatttagt gagattttat caaaagtata tatctccctt aaagggacct acctgtagat
     1141 tttatccaac ttgctcacaa tattcaatag aggcgtttaa gaaatatgga tttgtaaaag
     1201 gtatgtattt aacgataaga agacttttaa aatgtcatcc ttttcatcca ggaggttacg
     1261 accctctaaa ataaaagaga gtaactatta ggaggtataa aaaaattgaa tataatagga
     1321 aatttcttag gattgattct taaggtaata tttgaatttg ttaatcatta tggtatttct
     1381 ataatacttt ttacgatact tgtaaagatt attttattac cattaacaat taagcagact
     1441 aagtcaacaa aggcaatgca agatatacaa cctcgtataa aagagataca agaaaaatat
     1501 aaaaataagc cagaaaaaca aaatgaagaa attgttaagt tatatggaga agctaaaatt
     1561 aatccactat ctggttgttt acctttatta atacagtttc ctatattaat aggattattt
     1621 agcgttttga gagaaccagt tgctcatggc gtttttgcaa acaaagctgc atttttagca
     1681 gctgacaatg ggtttttatg gataaaaagc ttaacatcac cagattatgt attagcagta
     1741 ttttcaggag caagtgcata tgtcatgcaa aaagtaatga caccaaaaga tcagttacaa
     1801 ggctctatga aagtaatgac atatgtaatg gcaggtatgt ctttctattg gggatttata
     1861 ttcccagcag gacttacatt gtactggaca gtaagtaact tattctcaat agctcaatat
     1921 tacttaataa tgaatccttt aaaagctaaa ctggctgcta actctaagga ggaaaaagtt
     1981 aatgagaaaa agcctaaaat taataaaaag taagagtaaa gaggaagcta taagtaaagc
     2041 tatagcagag ttgaatttaa aagcagagga tatagaagta gaaatacttg aaaatccaag
     2101 taaagggttt ttaggtttta taggtgctaa agatggtact tatgaaattt ttgttatcga
     2161 gagggaagta gatgtagcta aaaattttat agaggtaatg ttgaaaaatg caaatgttga
     2221 tgccaaagtc aatgtatctc aaaaagataa tttaatcatg gttgatattg aaggcaaaga
     2281 agctgcaagt ttaattggta gaagagggga aactttagat tctattcaat ttttgactgg
     2341 attagctctt aataaaataa ataaagattc tcatacaaga gtacttgtag atatagaaaa
     2401 ttatagatca aaacgagaag aatctttaat aagatatgct aataaagttg ctagagaagt
     2461 agcgaaaaca agaaaaacta aaaaattaga ttatatgaat ccatatgaga gaagaataat
     2521 acattcagct cttcaaaatg acaaatatgt aattacatat agtgaaggaa ccgatccata
     2581 tagaagatta gtaattgagt gtaaaagata aagtcaacct cctaaacatt agggggtttt
     2641 attttttaat attattaaat cattaatggt tggttaaatg taaatctata tgtaaaaaat
     2701 tatgaattaa gaggtgataa atttgtttat agatgataca attgcagcaa tagctacagc
     2761 acctggagaa ggtggtatag gtatacttag aataagtgga gaaaaagctt tgaaggttgc
     2821 agaagaaatc tttaaatcta tgtctggaaa gagtattgaa gaatacaata agagaacatt
     2881 aatatatgga aatatagtgg ataatgaaaa tgtaattgat gaagttttat tggcatatat
     2941 gaaaggacca aattcctata caggagaaga tgttatagaa attaattgcc atggtggatt
     3001 tatttcggta aagaagatat tagaattaat actatcaaaa gatgttagat tagcagaggc
     3061 tggtgagttt actaaaagag cattcttaaa tggaagaata gatttatctc aagcagaggc
     3121 tgtaattgat gttataaaag ccaaaactga tatagctcat gaagttgctc aaaatcaatt
     3181 agagggttct ttatcaaaga agataagaga attgagagat aaagtcactg agatattagc
     3241 tcatgtagaa gttgctatag attatcctga ggaagatatt gagcatataa catatcaaac
     3301 tcttaaagaa aaaactgatg aacttaaaaa agacattaaa aaattatatg atacagctga
     3361 aagtggaaaa atacttagag aaggcttaaa gacggtaatt gttgggaaac caaatgtagg
     3421 taaatcttca ctacttaatt ctattttagg agagaataga gcaatagtta ctgatatacc
     3481 aggaactact agagatgtta tagaagagtt tgtaaacata aaaggtatac ctttaaaaat
     3541 tgttgacaca gcaggtataa gagatactga tgatattgtt gaaaaaatag gtgttgaaaa
     3601 gtctaaagaa tcatttacta gtgcagattt gatagtaatg gttttagatg catctagaaa
     3661 gttgtcagaa gaagatatag agatattaga aaaacttaag gataaacaaa caatagtgct
     3721 gttaaataag aatgatttga aacaagagat tgaagaagaa aaaatactta aatatgtaga
     3781 aaataatagt ataataaaaa tatcagcttt acaacaagaa ggaatagaag aattgcaaga
     3841 taaaatagag tctatggtat ataaaggtag tatcaaaaat aattctagtt tagtagtaac
     3901 aaattcaaga cataaagatg cacttagtaa agcatataaa tctgcaacag atgctttaat
     3961 tgctttggaa caaagtatgc catttgactt tgtagaagtc gatttaaaaa atatatggga
     4021 ctatcttgga tatattaatg gtgatacagt aacagaggat ttactagata atatattcca
     4081 taatttctgt attggtaagt aatgtacaaa agtaaacttg tttttattat agagattttt
     4141 aaaaaatttg aagtgtttct catgaaacat taattttgaa aagaaaggaa ttataaatga
     4201 taaagtttga agcaggaaaa tatgatgtta tagtagtcgg agcaggacat gctggatgtg
     4261 aagctgcttt ggctactgca agaatgggat ataaaacttt gataataaca atgtctttgg
     4321 attccatagc attaatgcct tgtaatccat caattggagg gactggtaaa ggtcaattag
     4381 ttaaagaaat agatgcctta ggcggtcaga tgggattaaa tatagacaag acatatatac
     4441 aaagtagaat gttaaatact gccaaaggtc cagcggtaca ctctttaaga gcacaagcag
     4501 ataaatttaa atatcatgaa gaaatgaaaa agacattaga agatgaacca aatcttgata
     4561 tagctatgga tgaagttgta gaaatacttc atgaaggaaa tgttgtaata ggagttggaa
     4621 ctaaactagg atgttcattt aaatctaaag ctgtaatatt ggccactgga gtttatctga
     4681 actcaaagat atatatgggt gaagttgcat tttatgaagg tcctaatgca ttagggtatg
     4741 caaaatatct tacagatagc ttagttgagc ttggccttag aatgagaaga tttaagacgg
     4801 gaactccagc aagagttcat agagatagta tagacttctc tgtaatgtct ttacaagagg
     4861 gtgatgaaaa agtaactcca ttctcattta tgaatgaaaa tatagaaaaa aaacaagaac
     4921 cttgttattt aacaagaact acagaagaaa ctcaaaaagt tatactggat aatttaaaaa
     4981 ggtctgctat gtatagtgga gtaatagaaa gtactggtcc aagatactgt ccttctatag
     5041 aagataaagt agtaagattt agtgataaaa catcacatca attatttata gaaccagaag
     5101 ggttaaatac taaagaaatg tatatacaag gaatttcaac tagtttacct tttgaagttc
     5161 aattagatat gtacaaaact ataaaaggac ttgaaaattg taagataatg agacctgcat
     5221 atgctattga atatgattgt gttgacccaa ctcaactaaa aataagtttg gaaataaagg
     5281 gtgttgagaa cttatttagt gcagggcaat ttaatggaac ttctggatac gaggaagcag
     5341 cagctcaagg gcttatggct ggtataaatg cagttagaaa gatagaagga aaagagccat
     5401 ttgtattaga ccgttcagaa gcttatatag gtgtcttatt agatgacctt gttactaaag
     5461 gaactaacga gccttacaga atgatgacat caagagcaga atatcgacta tatttaagac
     5521 aagataatgc agatatgaga ttaactcaaa aaggttatga tataggatta gttaaaaaag
     5581 atagatatga aaggttccta aataaaaagg ctgctgtaga gaaagaattt gaaagactaa
     5641 aaaatgaaag agttacacca aaagaagtaa atagtttgtt ggaagaaaaa ggagcaacac
     5701 caataaaggt tggaatatct ctatatgaat ttttaaagag accagaggtt acttacgaat
     5761 tgcttgaaga attagggaaa ggcgctggag aagatgtttc aagagaagtt aaagaacagt
     5821 gtgtaataat aactaaatat gaaggttata tagagaaaca attgaaacaa atagaccaat
     5881 ttaaaaaact tgagaataaa aagttggatg agaaaataaa ttattcatct atagaaggtc
     5941 ttagattaga agctagacag aaattagatg atataaaacc tatttcaata gggcaagcat
     6001 ctcgtatatc tggtgtttct ccagctgata tatcagtttt acttatatac ctagaacaaa
     6061 tcagaagaac tagaggtggc aaaggtgaat aatatagaac ttctgaaaaa cgggattgaa
     6121 ggttttaata tagatacgaa tgacagtatg ttagaaaaat ttaaaatcta tagagagata
     6181 ttagttgaat ggaataaaaa gatgaatctt actggaatag aagatgaaaa agaagtgtat
     6241 ataaaacatt ttttagactc tatttcagct gtaaaaaatg gatatataaa aaatgggatg
     6301 tctataatag atgttggtac aggtgctggt tttcctggaa taccattgaa aatatgttta
     6361 gatagcttag aacttacttt gttagattcg ttaaataaaa gaataaactt tttagaggaa
     6421 gttagtagag tactagagtt agataatatt acatttatac atggaagagc tgaagatttt
     6481 ggtaaaaatg aagactatag ggaaaaatat gatgttgcta cggcaagagc tgttgctgga
     6541 ttaccgattc ttatggaatt ttgtgtacca tttgtaaaag ttggagggta ttttatttgc
     6601 ttaaaaggtc cgaatgctaa tttagagcta gaagaatcac aaaaggctat tgatgtttta
     6661 ggattggagt atattgaaaa aatagatgta gagttaccag aggtagattt aaatcataat
     6721 atattagttt ttaaaaaagt gatggaaaca ccagttaagt accctagaaa agctggtaaa
     6781 ccagccaaaa atcctattaa gtaatgaaaa atttattatt ggtaagctta attatttctc
     6841 aggaaataat taagcttact gtaaaaaaac aaaaataaag gaattattaa agtagatgca
     6901 gaaataaatt tgtatgtaca tatagagaac aaaaataatt tatatctaaa ataagagagg
     6961 tatgttatgg aagataaaag agttatggag atacctatag aagatgtagt tccaaatcca
     7021 tatcaaccaa gaaaaatatt ttcacaagtt tcattggagg aattgagcaa ttctataaag
     7081 gtatatggta taattcaacc tataacagtt agagcaaaag atggcaaata tgaactaatt
     7141 gcaggagaaa gaagattaag agcagctaaa ttagcagagc ttaaaaccat accagcaatt
     7201 ataaataata tgaacgatga atcttctgct gttctagcgt tattagaaaa tttgcaacga
     7261 gaagacttaa atttcattga agaagcaatt ggttatgaaa acttaataaa agaacatgct
     7321 tttactcaac agcaattagc agaaaaattg ggcaaaaatc aatctactgt agcaaataag
     7381 cttagaatat taaagcttcc aaacgatata aaaatgaagc taatagagaa taatttaaca
     7441 gaaagacatg ctagagcatt tcttaaacta ccaagtgaag atttaatgca aagtgtgtta
     7501 gacaaagtca taaaaaatga attgactgtt aaaaaaacag aaaaattgat acaagatgta
     7561 ttagaagaaa ctaaagttca agaagagcct gataaaaaac agaatataaa gggtgctatg
     7621 agtataagaa tttacataaa tactataaag caggcctttg atgctatatc aaatacagga
     7681 attgatgcta agtataatga aatagataag ggagattata tggaagttgt tgtaaaaatc
     7741 cctaaaaaat agagattact gcttaccagt aatctttttt tgtaatataa gccttgtaat
     7801 ataaattgat ttatagacat tatttatact taaaaaacac ctattcaaca tttaaaatta
     7861 atttaattct taaagggggg aacatcatgg gcaaagttat agctgtattc aatcaaaagg
     7921 gtggagtcgg gaaaactaca acaaatgtaa atttaagtgc tagcttagga actttgggaa
     7981 agaaaatatt agtattagat ttagacccac aaggaaatac aactagtgga tatggtatta
     8041 ataaaaacga agtagaaaat acaatctatg aaattatgtt agatgggtta catattaaag
     8101 aagcaataat aagtactgaa tttgagaatg tagatgtagt tccatcagct actgaactat
     8161 caggtgcaga gatagagctt acatctaaaa ctaatagaga gtatatttta aaaaattcca
     8221 taaaagcggt aatagatgaa tatgattata tatttttaga ttgtccaccc tcactgggga
     8281 tgcttacaat aaattgttta actgctgtag atagtgtttt aattccaata caatgtgaat
     8341 attatgcttt agaaggtgtt agtcaattaa tggagactat aaaattagta aaatcaagat
     8401 taaatgctga tatagaaatt caaggtgttg ttttaagtat gtttgatgga agagctaatt
     8461 tatcaataca agtagttgaa gaagttaaga aatactttaa aggtagtgta tatacaactt
     8521 taattccaag aaatgtaaga cttgcagaag ctccaagtca tggaaaacct gtaatttatt
     8581 atgataaaag atgtagaggg tctgtagctt atttggaact agcagaagaa tttatagatt
     8641 tagaagagga ggaatggtag tatggagaat aagtctaaaa gaagtaatag attaggtaga
     8701 ggtcttagtg cattgatacc agaaattaaa ggagaaactt cagaaaaaga aatagtcaac
     8761 atagatatag acaaaatata tccaaacgaa gttcaaccta gaaagcaatt tgatgaagaa
     8821 aagataaaag tattgtcgga ttctataaaa aattatggtg ttttacagcc aatagtggtt
     8881 aaaatggatg aaaataataa atatatgata atagctggag aaagaagatt tagagcatca
     8941 aaacttgcaa ataaaaatca aatacctgct ataattaaag atatagatat gaaagatata
     9001 atggagatag ccttaattga aaacttacag agggaagatt taaattcaat agaagaggct
     9061 ctagcatata agagtttaat agagcattac aatgtaactc aggaagaaat atcagaagct
     9121 gttgggaaga gtagacctca tataactaat acactaagac ttttaaatct tggacaagat
     9181 gttatagaaa tgatagacag tggtagaatt acagcaggcc atggtaaggc tttattgaga
     9241 attgcagata aagatttgca acttcaaata gcgaaaaaaa tagaagaaga agaactttct
     9301 gtaagagaag tcgaaaatat agctaaaaaa atatctgaaa ataaacaaga agaacctaaa
     9361 aaatctaaac ctaaggatgt atttatttta gatgttgaag ataaacttag aaatatattt
     9421 ggaacaaagg tgaatatttc taaaggaaag aaaaaaggta aaatagagat agaatactat
     9481 aatgatgatg atttaaatag tattgtatca atgcttcttg aatagaacaa aaatataaaa
     9541 cagagaggaa gattgttatg atttatttag ataatgctgc aactacatat ccaaaaccag
     9601 aaagagtgta taatgctgtt ttagattgta tgaaaaatta ttgtgcaaat ccaggtagag
     9661 caggccataa acttgctatg agagctgcta gggagatata tgatacaaga gagaatatag
     9721 ctaagttgtt caatgtaagt aatcctatga atatagtatt tacatctaat gcaacagatt
     9781 ctttaaattt agcgataaaa ggtgtattac aagaaggaga ccatgtaata acaacgtcta
     9841 tggagcataa ttcagtaata agacctataa aagcattgga aaaaaggggt attgaaaata
     9901 ctgtagtaaa atgcgattat gagggatttt tagattatga agatttagaa aaatcaataa
     9961 agaataatac taaattgatt gtaacaacac atgcgtctaa tgtatgtgga actttaatag
    10021 atataaaaaa agttggagaa atagctaaaa aacataatat actattttta gtagatgcat
    10081 cacaaacagc aggtgtttat gatatagacg taaatgaatg taatatagat atgttggcaa
    10141 tgccaggtca taaatgctta tttggacctc aaggaacagg tatattatat gtaagagaag
    10201 ggttgaactt aaatatatta aaagaaggtg gaacaggtag taaatcagaa gaaatagttc
    10261 aaccagagtt attcccagat aaatatgaat caggaactca taatactcca ggtatagcag
    10321 gtcttaacca aggtatacta tttatatttg aaagaggaat caacaatata agacaacatg
    10381 aagaagaatt atgtcaatat atgatagaca aattagagga agtaccagat ataaagatat
    10441 atggaccaaa ggacagtaaa aaaagagcat cagtaatagc tctaaatata ggagatatgg
    10501 attctggaga ggttactttc ttattagata gtgattataa tatagcgact agatcaggaa
    10561 tacattgttc accactagca cataccactt taggtacttt aaaacaaggt gcagtaaggt
    10621 tcagtatagg atatttcaac acaaaagatg agatagataa agcagtggaa gctttgaaaa
    10681 aaatttcaaa aaataaatag catgagattt aataatatag aaataaaaaa aggggatatt
    10741 tcaaaataaa aatttgaatc atctcctttt ttgtacaaaa aagaaaaggg gttcctaaga
    10801 acctctaatc tatttgataa tagtttcaag aaattacttt aagtaatctc cttcttcaag
    10861 aactatatga ccactttcgt aattagaacc atcaacagta atttttattt gttcaacatt
    10921 catatttttt atgtaagtac gagcaactga atcaagcatt aaagtttctg catcactacc
    10981 taagtttgac tttgtaaagt cagagctaac atctaatata ccagttttta caccatcaat
    11041 gtcttttgta gtaaatgaat ttaattttgc agattctgga acaactttaa gtttttgaag
    11101 ttcttcaaat aaagtgttct catcaatttt atttaaatca actttatttt caattaaatc
    11161 tgtcttatct acatcaaaag agtaaatagt agctgattga gctttagttt cttcttttgg
    11221 agtaggttta gctttgttat taggaacctc tttttcagta ttgttttctt cttttggttg
    11281 ctcaacattt gtattgctct tagtttcttt tttagatgaa tcacttccat tttgagcatt
    11341 agtacaacca actgcgaata tagatataga taatatactt aacactaaca ttattttctt
    11401 atttttcata aaaatttctc ctctcttaaa aactattctt atatataatt atagtcgtta
    11461 tgagcaaata tatgaataca aaatagtaac aaatttaaaa aatgatttat ttaaattaat
    11521 aaaactcaaa ataaactaaa ttttagtcat ttttatggga aatcatttaa tgtacagtga
    11581 aaatattgta aaataaaagt agtgttttta aaataaatat aagttataat tatattataa
    11641 aaaaatgtct tcaaaaggtg ggaatagtat ggaaattttg agcttaggag aaaagattaa
    11701 aaagttaaga aaagaaaaaa acatgacatt gaaagaatta gctggagata ggataacagc
    11761 agcacaaata agccatatag agagagataa atctcatact agttatgaac tgttggaata
    11821 tctaggcgat aagctagatg taagtgtaga atacttatta gagaccaagg aaatgcaatc
    11881 aaaaaagata actgataatt taatactaca gagcgaaatt tatataaaag aaaatgaatt
    11941 aaacaaggct gaggaccaaa taaaagaagt tttagatata tgtaagagat acgatttaat
    12001 ggagaactat ggcagatgta attttttaat ggctaatatt aatttgaaaa aatctaatta
    12061 taatgatgct atagaaaact ttgaaaaggc attgtacttc tttataaaaa atagtgatag
    12121 tgaaaatata cttaagtgtt atttaaacat agggaaaata tatatgcagg aagactttta
    12181 taaaggtgct ataagccact ttaattttgc agaggaaatt ttgtcagaga gtaaatttga
    12241 ggatgttaac atacacaagg aactatatag taagatggca tattgttaca taaaactaga
    12301 taatccaaat atgtcattga agtacataga aaagataaat gaaatagata atagaaatga
    12361 tagaaaagaa gatgtcgata ttttggtgtt aaaagcaaat aatatgcttg agattggaaa
    12421 atatgaagaa tcaagagatt actttaaaaa agcactaaaa atacttgaga gtgaggataa
    12481 taaaactgga ttagcaaatg tgtatttaac aatttgtgat gtttacagaa aaataggtga
    12541 aacagataga gtacttgaat attcacaaaa ggtatatgac ataaagaaaa atgatgaaga
    12601 tgaatacatg atgtcaggat tatttaagat aatagaagca tatatagata aagaagatta
    12661 tgatttagct agaaaatatt gtaagatagc cttagcttca tcaataaaaa ataaaaataa
    12721 attcaatgaa tataaagcat tgagattcta tgctaatatg tataaaaatc agaatgaagt
    12781 aactcttgca attgaatatc tgattaaatg cataaaaata gtatcagatt taggcaacaa
    12841 tagaatattg gccaatttat atatagactt aggtcaatta tattcaaata tatcaaaaga
    12901 aaaagagtta gaatattatc aaaaaggtgt atttatgtac aaaaatttag aaataatgta
    12961 gggtaaataa acctataagg gtatagaaac aatctataac aagcaagata atatctatga
    13021 taaaatataa attgtatgaa tatttttaag gagatgaatc agacaatgag tataaaaata
    13081 gatgcgagag ggcttgcttg tcctaaacca gttataaata caaaaagaga attggataac
    13141 ctagaagaag gtgttgtagt tacaatagtt gataatgaaa cagctaagga gaatatatta
    13201 aaacttgcta aatctttaag ttgtgaagca aatattgttg acgaaaaaga gaattttata
    13261 tctatagaaa ttagaaaagg taaaaatata ctagataaac aaaatgttgt agataataaa
    13321 gaaagtgaat tagacaatac atgtatattt atatcttcag ataagatggg acttggaaat
    13381 gacgaattag gaaaagtgtt aataaaagga tttatatata cattaacaga atctaaacct
    13441 tatcctaagt atgtactttt agttaatgga ggagtaaaat taagtgctga aaatgaagaa
    13501 accatagaaa atctaaaaat acttgagaat atgggtgtag aagttttatc ttgtggtact
    13561 tgtttagatt attataattt aaaagacaaa ttacaagtag gtacagttac aaatatgtat
    13621 actatagttg agacattaaa gaatgcatca aataccattt ctatataatg tatgtatgtt
    13681 taataatata aaactaacaa taaagattaa ctcaaataga tagtcagaaa ggattaagta
    13741 atgaatcaaa tgtatatagt gtcatttaat tcgacacatc atgcgataag atcagaaaag
    13801 ttatttggtg aaaatagtct aaaagtaatg gctcttccaa caccaagaga aataacagca
    13861 agttgtggaa tatcaattaa attttctttt gaagatatgg aaaagataaa aactattttg
    13921 gttgaaaata atgtagatat aaaaggaatt tattgtataa gtaaattaga aaatggttca
    13981 aaagaagtag agaagttaga ttaggaggct aattatttat gccaatggat ttaaaaatag
    14041 gtgatattgt tgaattaaaa aagcaacatg catgtgggtg taaagagttt gaaatcataa
    14101 gaacgggaat ggatataaag ataaaatgta ctaaatgttc aaggctaata atgcttgata
    14161 gagagacctt agaaaagaga gtaaaaaagc taataaaaaa gtgataaaaa tttaacactt
    14221 aaaaaatgtc aaataccgta tattaatatc caaaattttg gtataataag aattgaagaa
    14281 aacattttca tgggggtgct ttaattatgg cagttaaata tgcaaaaaga atgcaaggat
    14341 tacaaggatc agaaatacgt gagcttttaa aacttactca acaaccacaa ataatatctt
    14401 ttgctggtgg aatgccggct ccagagttat ttccagttga ggaaatgaaa aaagtatcag
    14461 ttgcagtgct tgaagaaaat ggaagatcag ctatgcaata tactacaaca gaaggatatg
    14521 aaccattaag agaaaaaata gcagctagaa tgaatgataa aaacaaaact aatgtaaaca
    14581 aagatgatat attagttaca agtggttctc aacaaggtct agattttgca ggaaaagtat
    14641 ttattgatga gggtgatgta attttatgtg aaagtccatc ttacatagga gctataaacg
    14701 catttaaatc ttatcaacct aaattcatag atgttccaac agattctgat ggaatgataa
    14761 tggaagaatt agaaaagata ctagaaacaa ctgatagaat aaaaatgatt tatgtaatac
    14821 ctgacttcca aaatccaaca ggaagaactt ggccacttga aagacgtaaa aaattcatgg
    14881 aaatagtaaa taaatttgaa ataccagtaa tagaagataa tccatatgga gatttaagat
    14941 ttgaaggaga gactttacca tctttaaaat caatggatac aaaaggatta gtaatattct
    15001 taggaacttt ctctaaaata ttctgcccag gatacagatt aggatggact tgtgcttctc
    15061 cagaaatttt atctaagttt aactttgcaa aacaaggtgc agacttacaa gcatcaacta
    15121 tatctcaaat ggaagtaagc aaatttatgg atatgtatga cctagatgca catgttgaca
    15181 aaataaaagc agtttatgtt aaacgtagag atgtaatgct taagactatg gaagaagagt
    15241 tcccagaagg attagtattt actcatccag aaggtggatt atttacatgg gtagagcttc
    15301 caagtaattt aaatgctaaa gaattaatgc caaaatgttt agataagaat gttgcttatg
    15361 ttgctggagg aggattcttc cctaatggtg gtagagaaaa tactttcaga cttaactatt
    15421 caaatatgcc agaagaaaaa ataatagaag gtataaaaaa tatagcagct gtattaaaag
    15481 aagctatggg tgtagaagct taattaaaaa taataactta tatataatac aagggatggt
    15541 tgttgccatc ccttgtttgt ttgaaaagta tataattcac cttatactaa actttgtcca
    15601 aaatttaaat tgacagaaat aagaataaat gataaaatgt gtaggtatga gattttagtc
    15661 tcatttctct gctctaagag tgtagagccg ttaagtccaa agggaggtga attatagtga
    15721 gaaattatga attagtttat gtggtaaagc caaactctga tgaagaagta agagaggcta
    15781 tactaaacaa agttaaggaa gttgttgcaa ctgacggaga aatagttaaa gttgatactt
    15841 ggggaactaa aaaattagct tatccaatag ctaagtttac agaaggtttc tatgtattag
    15901 ttaacttcaa atcagcagta gacgttccta aagagataga cagaaactta aagataaatg
    15961 aaaacgtaat aagacacatg atagttgttg cttaataact ggttttatta taaaaaatag
    16021 gtggtgtttt tatgaatcaa gttgtattag tgggaaggtt gactagagac cctgaattaa
    16081 gatacatccc aggtacagga acaccagtgg catcctttac aatagccatt gatagagact
    16141 atgtaaaaaa agatggatct aaagaaactg attttatacc tgttgaagta atgggtaaat
    16201 ctgctgaatt ttgtgctaac tacataacta aaggtagatt agtagcactt caaggatcta
    16261 taagagtaga caattatcag acacaatcag gtgaaaagag aacttttaca aaagtaagta
    16321 ctagatcagt acaagctttg gatagtaaga ataaatcgga aaattcatat agagagagtg
    16381 ctccagcttt tgagccaagc tttgaacctc aaggtttaga cccacaaggt ttccaagcta
    16441 tagatgatga cgatatacca ttttaaaaag gagggaaaaa agtcatgatg aataaaaaaa
    16501 gacgtaagaa aaaaagagtt tgtcaatttt gtgctgacaa aaacgctaaa atagattaca
    16561 aaagtactca aagattacaa aagtatataa ctgaaagagg aaagatatta ccaagaagaa
    16621 tatctggaac ttgtgctaag catcaaagag aattaacagt tgctataaaa agagcgagaa
    16681 atatagcact tttaccatat acattagact aaaaaaatga gccttgtgct cattttttat
    16741 tatatgtatt tatatatcta aatgaggcga gctataaagc tcgttttttt gtttatatac
    16801 ttaaatatat tattatttat gatataatat tatatattaa ggagggttgt aaacgtgaaa
    16861 atagataaag gttctgagat aacgagaaac ataaagataa tagaatggat gaaaacagaa
    16921 atattaatga gtgtaagtga tttgtttaat ttattgttta aaggggtaaa accattagat
    16981 gaagcaattc aagatacttt agcaaatata ataatgataa catatctctt agcaaagaga
    17041 ttaggaatta gttttagaga tgtggattat aaagtcaagg aaaaaataaa aattggtata
    17101 gatgaggacc atagcgttga aagatggtat ggagatttat ctaatttaaa aaaacatatg
    17161 gataataggg agtgatatta aattgaataa taaaattaga ctatctaagg caatgtctat
    17221 aatagtttta gctataataa tcgctttagt aatagcctat attccaatgc taggaatatt
    17281 tagtatgtta gcagctgttc catatgtaat tataggtgct attactgata gaaagtattc
    17341 tcttatatct attttggtta cattttgtgt actgattcta tttgcagaca tttcttatgc
    17401 attaaatata tgtataatgt attctttacc aggtatagta ataggaaaga tgctaaagag
    17461 aagctttgaa caagaggaca gtaataaatt cgaaccgata tatggtggaa ccataatatt
    17521 tgtactttca atgcttgtat atttttttgt attaaagaca tttttaaatg tgaatctatt
    17581 agatgaagtt tctaagatga tatcagaaat tgtaaatatt caaaaaaata gtttttctgc
    17641 aactgaactt aaagtgtttg ataaaatgaa gccagatgag attgtaagtt attttacaaa
    17701 tatgctacca atgatgttat ttttacaagg gttgttatca gcgtttgtca catattattt
    17761 gagtgtattt tttataaaaa ggattacaaa gatgaatata agatttccta agtttgctga
    17821 tttttattta ccgggaaatg ctatactcac aactttctta ctttatttat tagtcttatt
    17881 tattcaaatt attggttcaa aattatatac agaattaata atgactaatt tacagctagt
    17941 ttttaattta atgtttgtaa ttcaaggtat agcagtttgc atttatttct taaagaaatg
    18001 gataagacag ggaccaaata aaattatgtt tttttcaggg ataattttat gtttatttgg
    18061 atttatggga atatcttttg ttggtatggt agatagtata attgacttta gaaaggtaag
    18121 aagctataaa tccacttagg aggatagaaa tgagtaataa acaaaccttt aaattaaata
    18181 tgccagaaat aaatttatat ataattgtta ttggtatttc tagtataatt ttgctttatt
    18241 acaacttgta tgtagggtgt ttatttttct gtatttttgt atatatggtt tttcacaatt
    18301 ggagaactac taatattaga cgtcatgaat ggacagagta tattcaaaat ttatctttag
    18361 atatagatga aacaactaaa aaagccataa taaatcttcc aataccatta tgtattttag
    18421 aatttgatgg aaatatatcc tggtataatg gaaagtttta tgatatgatt ggtcaaaaag
    18481 atttacttga taaaaatata gaggatatag ttaaaaatct taatttaaga aaagtattaa
    18541 atgaaaataa agagatgtac acagaaataa actataaaga aaaagaatat acaataattt
    18601 ataatgttat aaaaaatgat caagaaaaaa atcctaaata tttgatgata ttatactgga
    18661 tagacaagac tgaatattta aaagtaaaac aaaattatga tgatgaaaaa aatgcaatga
    18721 tgctaataca agtagatgga tatgatgaag tattaaaaag tgcagcagaa gataagagag
    18781 ctttaattaa tgttgaagta gagaaaatac tttctgcttt agaattaaat tcaaatggtg
    18841 cattgagaag aacttcaaaa gacaaattct tcttggtaat gcataaaaaa gagttaaaaa
    18901 aattggaagc tgagaagttt tctattttag atacaataag acatattgat tatggaaata
    18961 atcttcctgt tactataagt ataggaattg gtatagatgg agatacatta aatgagaacc
    19021 taaaacttgc tacgggtgct ctcgatttag cacttggtag aggtggtgac caggctgttg
    19081 taaaaacaaa agataaattt gtgttttatg gaggaaaatc taaagctgta gaaaagaaaa
    19141 ccaaagtaaa atccagatta ataggtcatg ctttaagaga ggttatacaa caaagtgacc
    19201 aagtttatat aatgggacat aaatatcctg atatggatgc tatgggagca gcagttggtg
    19261 tttatgatat atgtaaatcc tgtaacaaaa ctgcaaacat agttttacaa tctgtaaatg
    19321 aatctataga aatatttata aataaaataa atgaaaataa ttactataaa aagttgttta
    19381 ttggaaaaga ggaagctatt gacaactgca ctaaaaatac attagttgta gtagttgata
    19441 ctcatagacc aaattatact gaatgtgaag aactgttaaa gctatcagag aaggtagtag
    19501 ttatagacca tcatagaaga ggtgtagagt ttataaatga tgcagtgctt ttattccatg
    19561 agatatatgt atcctcaacg tgtgaaatgg ttacagagtt agttcagtat atggatgaag
    19621 atgtaacaat aaataaactt acagctgaag ggcttttagc aggtataagt ctagatacta
    19681 aaaactttgc attcaaaaca ggtgttagaa cttttgaagc tgcttcttac cttagaaaag
    19741 ttggagcaga tactatagag gtaaagaaat tcttcaattc tgatgtaaaa gatttcataa
    19801 taaaagctga aataattcaa agtactaaaa taattaataa tagaatatgt ttagcatatt
    19861 caagtactga gatagatagt ataaatgtta taatagctca aactgctgat gagttattaa
    19921 atatcaaaga agtagaagct tcctttgtat taggtgaaaa agatgatact atatttataa
    19981 gtgcgaggtc tttaggacaa ataaatgtac atgtacttat ggaaaaatta ggaggcgggg
    20041 gacacataga tatagctgga gcacagttaa aaaatgtttc tctaaaagaa gcttataaga
    20101 tggtaaataa aataatagag gagtatttgg aggaggaaga atagatgaaa gttatattat
    20161 taaaagacgt aaaaggaact ggtaaaaagg gagaaatgaa agaagtaagt gatggatatg
    20221 caagaaattt cttatttcct aaaaagatgg ctgtgcaagc agatagtgta gcaataaaag
    20281 aattaaatga aaagaataaa tctaaagaaa taaaagcaca aaaagagtat gaagaagcag
    20341 ttttattagg taagcaaatg gaagaaatta atatagaaat atattcaaaa gcaggagaag
    20401 gtggaagatt atttggttca ataacttcta aagaaattgc tgagcaatta aagaaacaaa
    20461 aagacataga tgtagataaa agaaaaatat tattagatga accaataaga tctttaggtt
    20521 caactttcgt agaaataaaa atacatcaaa aagtaactac taaaataaga gtggatgtaa
    20581 aagaaaaaca gtaataacta gaggtgatta agatggaaga tatgacgaga attcctcctc
    20641 atagtgtaga atcagaacaa tcaatattgg gttctatact tctagataaa gatgctataa
    20701 taacggttac agaaactata aagcctgatg atttctataa agaggctcac aaaataatat
    20761 atgaatgtat gataacttta agtaataaag gtgagcctat agacttgata acattaacag
    20821 aagaactaag aaaacaaggg catcttaatg atattggagg aatcagttat attacaagtc
    20881 tttctactat agtaccgacg acttcaaatg taaaatacta tgctgatata gtaaaagaaa
    20941 agtctgtact tagaaagctt ataaaagctt ctaatgaaat aataaatcta ggatatagtg
    21001 gagcaactaa gattgaagat gttttagaac aagctgaaaa gagtatattt gatatatcac
    21061 aggagaaaac tagtgatgat tttaaatcta taaacttagt tttgatggat gcctatgata
    21121 tgatagaaaa attatacact aacaagagtg atgtcactgg tataactact ggttttaaag
    21181 atttaaacaa aaaaataaat ggtcttcaaa ggactgattt aatattaatt gcagctagac
    21241 cagcaatggg taaaactgcc ttttctttaa acttggttca aaatgcagcc ttaaaaggtg
    21301 atgcctctgt agctgtattt agtttagaga tgtctaagga acaattagta caacgtatgt
    21361 tatcttcaca atctagtgta gaattaaaaa aaataaagac tggaacactt aatgataatg
    21421 attggcctag aattatagat gctatggcag tattatcaga tgccaaaata cacatagatg
    21481 atactcctgg tataaaaata tcagaactaa ggtcaaagtg tagaaagtta aaaatagaaa
    21541 aaggactaga tttagtgctt attgattatc ttcaacttat ggaaggtgaa ggtaataatg
    21601 aaagcagaca acaagagata tctaagatat caaggtcact aaaaatatta gcaaaagaat
    21661 taaactgtcc tgtagtagca ctatctcagt tatcacgtgc accagaacaa agagcagacc
    21721 atagacctat gttatctgat ttaagagagt ctggagcaat agaacaggat gcagatatag
    21781 ttatgttctt atacagggac gaatattatc atgctgattc tgagagtaaa aatataggag
    21841 aagtaataat agcaaaaaat agacacggtg aaactggttc tgtggaactt gtttggcttg
    21901 gtgaagttca aagatttggg gataagttga gagacctata atctaacgaa tatccacaga
    21961 acgtcctttc tttctacagg aaaaatgtgg atacatgtca ctaaatatgt ccaaacattg
    22021 aaatttagct aaaaaactat ccacactatc cacagatggc ctgttgataa gtgtgaataa
    22081 gtagataaag caagcttttg gcttgcttta tctgtgtata aatattaatt attaggagga
    22141 ataatatgga aaaaataagc ttaaaatata tatatcccaa tataattaaa gtacttgatg
    22201 aaataaactt atttagagta attgacaata atttaagaga atctattgtt gtttatgcta
    22261 ataatgtgga taatcagtat catataaaca tgactaatac aaactttgga aatataatta
    22321 acatatgtaa attagaaaaa cttttagatg tggataaatt tatggaaaaa gttataaaat
    22381 atgaaaaaga aataatagag aaagaagagt tttcaaaaat agaagaatat atgttaaata
    22441 ttggagaata ctaaaaaagt tgaaaaaaat acgaatgttt gatagaataa gtaagtaaag
    22501 tgttcgagag tatataaaat atagaggtga attacatgaa aacagttgca attgttggtt
    22561 ctcaatgggg agatgaaggt aaaggtaaag ttatagatta tcttgctact caagcagatg
    22621 tagtagtaag agggcaaggt ggaaataatg caggacatac attagttgta gaaggtaaaa
    22681 aatacgcatt acatctaatt ccatctggag ttttaaatcc aaatacagtg aatataatcg
    22741 gaaatggaat agtgtttgac cctaaaggat ttttagaaga attggaaatg tttaaaacgg
    22801 ataatataag tacagaaaat ataaaaataa gtgatagagc acatgtcata ttcccatatc
    22861 ataaagaact agatgcattg tcagaagaag ccagaggcga tttaaagata ggtacaacaa
    22921 aaaaaggtat tggaccttgt tatatggata aaacagagag atctggtatt agaatatgtg
    22981 acttaatgga taaagataaa tttgctataa aattaaaagc tcaaatagat gctaaaaatg
    23041 aaatagttaa aaatatatat ggaaaagaag aattatttga ttttgaaact atatataacg
    23101 aatatctagg atatgcagag caaataagaa aatatgtggc agatacgtct gttattgtat
    23161 atgatgcagt aagagcaggt aaaaaagtat tgtttgaagg agcacaaggg actttattag
    23221 atttagattt aggaacttat ccatttgtta catcttctca tccaacatct ggtggatttg
    23281 caattggtgc aggtataggg ccaaatatga taaaagacgt tgttggtata gtaaaagcat
    23341 atactacaag agttggagaa ggaccgtttg ttacagagca aataaatgaa actggagata
    23401 agataagaga acaaggacat gaatttgggg taactactgg tagaccaaga agatgtgggt
    23461 ggtttgatgc agttatagtt aagtatgcag ctagagttaa tggattgact agtatttcat
    23521 ttatgttact tgatgtatta actggatttg ataagataaa agtttgtacg tcttataaaa
    23581 tgggcgataa gataataacg gatttcccag cttcattaga tgatttagca aaatgtgaac
    23641 ctgtatatga agaattagat ggatggaatg aagatataac tcaaatagat aactttgatg
    23701 accttccaga aaatgcaaag aaatatgtag caaaaataga agaactagtt ggagtaagtg
    23761 tagatatggt ttctgttggt ccaaatagag ctcaaactat aataagaaga aatatatttg
    23821 cttaatattt tatcaattaa ataatttaaa atagttgaag aagagtatct caagataatg
    23881 tttatagtta ttagagatgc tcttttatat ttgacttaca ttgatttttt attaagttaa
    23941 agctacttat acatattcta actgaaatta aagttgaatt tatgtagata catataggtt
    24001 ttttaaaaag aattttgttg aaaataaatg gctaaagatg attttaatta tattctaaaa
    24061 aaagtaaaaa aaactattga ctaaaaatta tatagacgct ataatataac ttgtgagtga
    24121 aagaaacgac gaaaaaagtc gaaaaaatgt ggtctattag ctcagtcggt agagcacctg
    24181 acttttaatc agggtgtccc gcgttcgagt cgcggataga tcaccaaaat atggctcgtt
    24241 ggtcaagagg ttaagacacc gccctttcac ggcggtaaca ggggttcaat tcccctacga
    24301 gtcaccagta tgggactata gctcagctgg gagagcacct gccttacaag cagggggtca
    24361 caggttcgag ccctgttagt cccaccaaat tttgaattag agataaatac tagattatat
    24421 gctggtgtgg ctcaacggta gagcagctga cttgtaatca gcaggttgta ggttcgattc
    24481 ctatcaccag ctccacaaag tacataaatt caaattaata taattttcta tgttacagga
    24541 tacttttaag tatctttttt taatttcatt tttattgtat taaaaatgaa attaaacata
    24601 caacaaaatt ttgaattact tgcaatgtga tagttttaaa tataaaattt agaatcattc
    24661 tagcataata ttatgtaata tagcaaattt aatttatagt atcaataaat atagactact
    24721 aagaatgtaa atgatttgtt agattaaggt acattagata tgtaaataga aaaataaaaa
    24781 gtatgtttta atttatgtga tataagtttt aaacagagaa gcaagtttgc tcttacctct
    24841 ctatttaaaa ataaaaaaat gttttttaag ggctatttta tttaaatctt tcccatctca
    24901 aatcatctcc agtaaattct aaaatcctaa acttatctaa tattctagag agagttcttt
    24961 gagtgtaagt atttcctatt tgagatggtg ttaagttggt tgaaataatg atttttttac
    25021 cagcaacaag tcttgtattt acaatattga agatttctcc actagtaaaa gaattattaa
    25081 gttcagttcc aaggtcatct attattaata aatcacaatc aaacaagttt ttataattat
    25141 cttcacttat ttgattattt gtgtctcttc taaatttata atcttcaaga atatctaaaa
    25201 ttctaaaaga agtttgatag attacaacat tgcctttatc taacaattcc tttgcaatac
    25261 aattgcacat ataggtcttt cctagtccag tagaaccata aaacagtaga ttttcactat
    25321 tatccttctt aaaatcatga acaaaatttt cgcagatact taaattattt agcatatttt
    25381 ctctaggaga tatttcacca tcagagcctt tcttaggaga aaatatattc aagttaaaat
    25441 tcgaaaaatt ttcctgactt aaaatcctat ctaaattaga cattttataa gcttcattta
    25501 caatttcttg tttgaggcaa gaacattttt ctccattagg taaaaatcct ttatctttac
    25561 aagaaataca ttgaaacttt atttccaagt aatcaagagg tatgttactt tcagctaaaa
    25621 gtctttcttt ctttactttt aaagattcta tattctcttt agtcttcttt acaatttcat
    25681 ctttagactt aggatttaaa agtactatct tagctaaact gagacctatt ttagaaattt
    25741 catcatctat agattttatc tcagggattc tattatatac ctcatttttt ctatgttcta
    25801 ataagagttc attatcatct cttctcttag catatttagc aagtattttt cttattttat
    25861 cctcattcat ttaatcacct acttaaactt ttctttttga ctttttttaa taatctcatc
    25921 gagctcatca gaggtatact gagtgaatgt ttcgttgaaa ttatgaaact tagttttttt
    25981 gtaagtattg ttgttttgag tagtattaat ctgcttctta ttaatattct ccttaactat
    26041 acgttcttcc tctttttgct ttaaatcatt taaatttttt atattttttt cattccaatt
    26101 tttaattata ccatttatat aagatatact tgggtttgaa atattcttag acttagaaca
    26161 tgcatttatt atcaaatcta tatccatatt aaatttatca aaccaggtgt ccataagttc
    26221 tttttctgat tttgatggtt gtcttgaaaa tcccaattca ttgaaaatcg ttttatataa
    26281 aatatatcgt tcacttctta ccaaaaagct ttcttcaaca tcttttggcg tatataaatt
    26341 tgaatcatac caatttctaa taatgccttc aatgtattta actggctttg atgtaccagt
    26401 tttatctttg acgtattcat atgcgcaaag tatcatatcg gggctcatat tgtatttatt
    26461 cattatatcc ataatactca tcttttcact tgggtcgaga tatcgcccaa ctattttatt
    26521 tatagaatta aacatttttc taatacttgg attttcagaa gtagatacaa ctttatctgt
    26581 attagatttt atagatgaat tattgcttaa tatattctca atatagaaat tttttaaatc
    26641 taaaaattct attgaataat taaaattatc atattcttca ttgtcatgta tttttataat
    26701 cttcttttct tcccagaatt tccaagcaga taacacatca gacaatggta tatttaggtt
    26761 tttggctatt gaattattgt cgaacttagg attagaagta atatcacatg cttgtctata
    26821 tcctaaaaga taaactttta cgtataaccc gtccgccatt ggcataaata tatctataaa
    26881 gatattggga atagtgattt cacctaaatc tatttcattg ctttctttaa aaaacatttt
    26941 atcacctcag attaaattat atcatttttt atattatatt tgtctgtaaa caaataaaag
    27001 gtcacaagat tagcatgtaa atattttcta aaagtattca ttttgaaaag gaggagatat
    27061 tttggttata aaattcaacc aaaaagtaag aagaacatta tcagtagcga ttctatttat
    27121 ggtattaata ataggttgct ttatagaaaa ggatgacagt ttagaaacat acaaagaaaa
    27181 aactaattca aaaataaatc agtataatat ggatgttata tttgatgatg agactaagag
    27241 gttaatgtgc aatcagaatg ttgactacat aaataataca aaatcaaaca tagataaaat
    27301 atatttccat atatatccga atgctttttc caagaaagat tttgctcctt ttgaaaaaag
    27361 tgaaatgaca agggcttatc ctaatggatt taacgaagga tatatagata taaagaatat
    27421 attaaataat aatagtaaaa tggagtataa aattaaagga gacaaaaatg acatattaga
    27481 aatagaatta gggaaagagt taaaaccaaa tgaaagaatg tcattagata taaaatataa
    27541 cgtaaaaata ccaaattcaa ttggaagatt tggatatgga gaaaatacta taaatgttac
    27601 taattggttt cctatagctt gtgtaaatga tgaaagggga tggaatttaa agagttatga
    27661 aactattggc gacccatttt atagtgagac tagcgatttt catgtgaaac tattgattcc
    27721 aaataaatat aaaattgctc atacaggaaa acttattgat gataaacatg ataataagaa
    27781 aatgctatat gaaatagaag ctgaaaacgt tagagatttt gcatttatac taagtagtaa
    27841 atttgatgta aacaaagtag attcaaaggg agtagctatc aatacatata atttaaataa
    27901 aaagctttct aaaaaagcaa ctgaagtggc aaaagattca ataaatatat ttagtgaatt
    27961 atttggaaag tatccttaca aagaattttc tgttgtagca agtgactttt ttataggggg
    28021 gatggaatac cctatgcttg ttatgataga ccaaagttta tataatgaaa aaaatgaatt
    28081 tttactagaa tatgttattg cccatgagac agcacatcaa tggtggtatt ctgctgttgg
    28141 aaatgatgaa ataagtgaac cctggttaga tgaagcactt actgaatatt ctacaatagt
    28201 ttattttgaa caaaagtatg gtaaagatat ggcaaacaag cttataaaaa ctatggagat
    28261 acagacaaaa agttatttga gtgaaaatat atttaagcca gcaaatgagt ataaaaattc
    28321 tactgaatac agcttgaatg tttatacaaa aggcgcaata gcatttaatg aaataagaaa
    28381 agaagttgga gataaagtat tctttgagac tttaaatgag tactataata aatataagca
    28441 taaaaatgct aatggaagag cttttgtgga actatggaat agtaaaggtg tagatataaa
    28501 taagattata agtgaatgta agtaaatagt gaatgtaagt aaattttgtg cagagtatta
    28561 aattttattt aatactctgt ttttttggta taatataatt tataaggcag aaaaagaggt
    28621 gaaggaatgc taaagtattg ttcagtaggg agtggtagta gtggtaattg ccactatata
    28681 gggtataaaa atactaatat actagtagat gcaggattaa gtggcaagag aataactaca
    28741 ggtcttaaag acattggtgt agatgcagat aaattaaaag gaatatttat aacacatgag
    28801 catgttgacc atataaaagg agcaggtata ttatcaagaa agtttgatat accagtattt
    28861 gctaatataa aaacatggtg ttctatgaag gacaaattag gtgacatcaa agataaaaat
    28921 atgaaagtat ttgaaaatga taaaacctac tcattaggtg atatcatagt aagacctttt
    28981 tcaatagaac atgatgcgtc tgacccagtt ggatataatt tttatacaga gagtgatgaa
    29041 aaaatgtcta tagctactga tataggatgt ataactcaaa atataaaaaa acatctatat
    29101 aaatctaaat tagtagtatt ggaatcaaat tatgacccta atatgcttat gatgggttca
    29161 tatacatatg ctttaaagaa aagagtaatg tcaagtactg gtcatttatc caatgaagat
    29221 gcagctaatt tctgcgttga acttattaat gaaggtacag aatctatatt attagcacat
    29281 ttaagcaaag aaaataattt tcctgagtta gcgtatgaga cttcaaaagg agtattagca
    29341 tcaaatgata tagtagttgg acaagatgta aaactagatg tactatcacg aaatgatgtt
    29401 tctaatgttt atgagatgaa gtagaggtga ataactttgc taaagagaaa ttttgaaaat
    29461 gttgaggata ataaaatttt atactctata aagcagggta tgattttagc gatacctgct
    29521 attatgactg gttcaactgc tcttgtaatt ttgaatcttc caataaaggc ttatcaagaa
    29581 tatttatcag gcttgtttaa tggagaggtt acaaatattc ttaactttat aaatgattct
    29641 actttaggaa ttatttcatt aataatatta ttaacaataa gctatagtta tggaaaaata
    29701 tatgggagca agtatacagt acttgttcct atagttgcaa tgtgctcttt tttagtattt
    29761 tcacatggta atgagcttaa ttcttatata gaaatattta aaacaaagtg gttatttaca
    29821 tctataattg tttctatgac atcttctgta ttgtttgtaa aacttacaga atcttttgtt
    29881 acaagtgtaa agtttcatac agaaggggct gatgcagact ttaatatggt agtttcggct
    29941 atagttcctt tcataatagt agtatttttg ttttctattt ttagagtggt aatgataagt
    30001 ctaattggca gttcaaactt tcaagatata ttttataatt tattttcaaa tgtttttaat
    30061 aaaatgggaa caaaccttat gagtgctctg ttgtttattt ttttaatgca ctttatgtgg
    30121 ttttttggga ttcatggtag taatgtatta gataccgtcg caaaaaattt atttgaaaat
    30181 agtatggcaa ttaacataaa tttagttaat aataatcaat taccaacaga aatttttact
    30241 aagacctttt ttgatacaat ggttttattg ggaggatgtg gctctttact atgtttagta
    30301 attgcaattt ttcttagtga aaaacgtata aatgtaagaa agttagctaa aattgcttca
    30361 ataccagctc tttttaatat caatgagatg ctagttttcg ggataccaat tgtttttaac
    30421 tatattatgt ttgtaccttt tgtaattact cctataatct taaccgttac aagttatgtg
    30481 gctatgtcta caggtattgt accatgtaca gtttcagctg tagaatggac ctcacctata
    30541 tttttaagtg gatatatgtc tacaggttca ataagaggta gtttgttgca agtatttaat
    30601 ttatgtgttg gtgttatgat ttatattccg tttataaaaa tgtcgcaaaa tagatataca
    30661 tatatgttta aaaataatat agaaaattta acaaacttat taaaaaggta tgaattaaca
    30721 ggtgaacagc ctaatcttct aaaccataat ggtaaaatag gttcaatttc aaaaatgttg
    30781 gcatcagact tgaagtttgc tatgaaaaga ggagaaatag agctttttta tcaaccacaa
    30841 gtcaattata atggaaatgt tgtgggagca gagggattac ttagatggaa acattcaata
    30901 ggagggttca tttatccacc actagttata attttggcta aagaagaagg gttcttagat
    30961 gagcttggag agtatattat taataaagca tgtattgatt taaagaagag tgaaaagcta
    31021 ttgagaaatc ctgttaagtt ctctgtaaat atatctccag accaacttga tgaccctaag
    31081 ttaccagagc aaataaaaaa tatcattagt agtaatgata taaatcctaa tatgttaggt
    31141 atagaaataa cagagcaaat tgcattatca ggttcacaaa taattattga acgtatcaat
    31201 gcgatacata acttgggaat aaagttaatt atggatgatt ttgggatggg gcatagctct
    31261 cttatatatc ttcaaaataa taactttgat atagttaaat tggatggttc attagttaaa
    31321 gagatactta caaataaaag aagtagtgaa attataatga ctattgtaaa tctttcaaaa
    31381 aatttagatt ttgatatagt tgttgagtat gttgaaaata tagaccaaag ggataagtta
    31441 tatggtttag gatgtgaaat atatcaagga tattattata gcaaagcaat accttttgaa
    31501 gagtttgtag aatatgcaaa tcaagaaaga gagatataat tttataagag cttgtaaatt
    31561 ctagtaatgc atagatttgt aataaaatta agtttaggag aaatatatga atataagtat
    31621 agtagtagta ggtaaaatta aagaaaaata tttaaaatta ggtattgatg agtttaagaa
    31681 aaggctttct aagtattgta agttagaaat tatagagttg gatgatgaaa aagctccaga
    31741 gaatttgagt gttaaggaaa tggaaattat aaaagacaaa gaaggaaaga aaattttagg
    31801 gaagataaag cataatagtt atgttatagc cttggctatt gatggtaagg gtttatcctc
    31861 tgaagagctt gctaagacta tgagtgattt agcagttaga ggtaatagta gtctctgttt
    31921 tataatagga ggttctttag ggctttctga tgaggtttta ggaagggctg attataggtt
    31981 atccttttct aagatgactt ttcctcatca gatgatgaga ttgatattgt tagaacagat
    32041 ttatagggct tataggataa ataatggaga accgtatcat aaataaatga tacggttttt
    32101 gttaatttga cacttaataa tattaaaata acaaaaaagt attgaaagta tattgcattt
    32161 aaggtagaat taaataaaat attataacat tataatgttt atatggttgt taataaaaat
    32221 ttggaggaaa atatatgata aaaataatgt tggcctgttc agcaggaatg tctacaagtt
    32281 tattagttac taaaatggaa agtgcagcta aagaaaatgg tatagagtcg caaatttggg
    32341 caataccaga atcaactata caaaatgaaa tagaaaaatg tgatgtatta ttactaggac
    32401 cacaagttag atatgtatta cctaaggctc aagaaattgc taagccatat aatataccag
    32461 tagaagttat aaatatgatg cactatggaa cagttaatgg agaagctgtt ttaaatagag
    32521 cgatagaact taacaacact aagtaatata aattataatg tatttttaat atataaattg
    32581 ggagaatcaa aggatggatg aaaagactat agaaatatct tttaatataa taggttatgc
    32641 aggtgaaggt aaaggattag cttttgaagc aataaaagag gctaagaatg gaaatataga
    32701 aaaagcaaaa gaattattaa aagagtccag ggaagttata aataaagcac atagatatca
    32761 aacagaatta atacaaaacg aagctagtgg aaacaggact gaaataagtg tcatacttat
    32821 acatgcacag gaccacttaa tgaatggcat gaattttcaa cagttagctg aggaaatcgt
    32881 tgatttacat ttgaaattac aagataaata aaaattaaag ataataaatt aagagagttc
    32941 ttaaaagata cattatgtgt agcacactaa ttgttagatt attgattcaa tatttagtga
    33001 gtaatacatt aggattattt tgaggactct ttttatattt agtttaaagt taataaatat
    33061 ttattattaa taatataaga ttatatgtta taatattata acatataatt taagaggtga
    33121 atcatgttaa gaaatcaacc attatatata caagttgtag atatattaaa gcaaaaaata
    33181 ttaaataacg aatataaaat tgatgaaaat attccaacag aaagggagtt agagcaagag
    33241 tttggagtca gtaaaattac tattagaaaa gctattgaga tattagaatc agaagggtat
    33301 gtagagaaaa aaagtggaaa agggacaaaa gtaataagta atagtctttt taataagcta
    33361 tcaaaagcag agtctttttc aagtatactt ttatctaaag gtcataagtt atctaaaaag
    33421 actatttcaa ttgaaaaaat aataaatgat acaaattcac aagtatttag tatatttgga
    33481 gaaaaatgta gcaagattac tagaatgtat tacttagata gtaagccata tatatttttt
    33541 actcattata ttccttttga tgaaaatcta actgttgaaa gttttaataa tgaaagttct
    33601 ttatatatgt acttatatag gagaaatata aatataacta gatttgaaga tgaatttttt
    33661 atagaagaat cagaactaga ggtatcaaag gctctaaatc tagctaaaga catattatta
    33721 ggaagaaaaa gaaagaccta tgatgagaat gataatatag ttgagctatc atttgcaaga
    33781 tataatacag aagtacaaaa ctatgtaatt aaatacgaaa tataataaga atcctgagtt
    33841 tattgaaatg ataatatgct caggattttt gtttagttgt aaaaataaaa aatattataa
    33901 cattataatg tttacaacta aacattataa tgttataata tttttaataa acaagtgata
    33961 ttatcaatta cataaaaatt agtttatgtt atgaaatcgt tattgcatta taaataaagg
    34021 ataatatcaa taatacaggg ggataactat ggaaaaattc gcaagttttt tagagcgcaa
    34081 gattatgcct gtagcagcga agataagtaa tcagagacat atgagagctg taagagcagg
    34141 tataatagca acattaccac taacaatagt ggggtcattt ttcacaatat tattaaacat
    34201 acctattgat gcttatatgg attttatagc tccatttaaa gcaatattag atataccatt
    34261 tagatatact gtaggatttt tatctctata tgctactttt gggatagcat catcacttgc
    34321 taaaagctac gatttagata caacaggtgt tggtatgctt gcagttatgg catttttagt
    34381 ttctacaata gttccaactc aagtagttga accagtaggt ggagttatag aagctggtag
    34441 atggatggat atggcaaaac ttaattcgcc atcattattt ggagctatcg taacatcatt
    34501 aattacagtt gaaatatatc gttttatgaa ggaaaagaat attacaatta agatgccagc
    34561 aggagttcca gtagaggttt caaattcatt tactgcatta ttaccgacag cagtaatttt
    34621 aatgttattc tgggtaataa gacatatttt aggatttgat ataagtgttg cactaagcgc
    34681 tatgttatca cctttaaaag gattcttatc aggaaatagt ttaggtggtg ggttactgac
    34741 agtattccta ataactttat tctgggtact tggaatacat ggtccagcta taatggggcc
    34801 agtaataaga cctatatggg acatgtctat agcagaaaat ataactgcat tcacagaagg
    34861 agtatcagct catgcaatgc caaacatatt tacagaacaa tttttacagt ggtatgtatg
    34921 gataggggga gcaggggcta ctcttgcatt agttgtaatg tttatgtttt ctaagtcaca
    34981 atatctaaaa agtttaggta gattaggatt tttaccaggg ttattcaaca taaatgaacc
    35041 tataatattt ggagcaccaa tagttatgaa tccaatacta gggataccat ttataattgg
    35101 acctataata atgacaattc tatcatatgt tcttactatt acaggggtta tacctatgat
    35161 ggtagcaaga ttaccatttg caatgccagc accaatagca gcagtaatga gtactaactg
    35221 gagtatatta gcaggtatct tagtattaat caactttgtg atatcatttg ctgtttatta
    35281 tccattcttc aaagtttttg aaaaacaaca attacaaaga gaacaagaag aaatgagtga
    35341 aaatttataa aaatacatag atatagaggt tttgtactat ataaaatata gaaaaaactt
    35401 ataagttatt aagggtgaag gtaggaggaa tgacctttgc ctttaatttt atatatttta
    35461 aataatgtaa tttgaggagg agaaagatgg aaagaagatt aggaatatca gtatatccag
    35521 agcatagtaa catggaagat gataaggcat atataaaaat ggcttcaaaa tacggattta
    35581 aaagaatatt tatgtgcatg ctttcaatta caagacctaa ggaagaagtt aaaaaacatt
    35641 ttaaagagat tataacctat gcaagagaat taggatttga agtaatcctt gatatagctc
    35701 caaacatatt tgatatatta caaatatctt atgatgattt aacatttttt agtgagttaa
    35761 atgctagtgg aataaggctt gactatggat atgatggaag taaagaagct atgatttctt
    35821 tcaatccata tgatttaaaa gtggaactaa atatgagtaa tgatgtagca tacttggata
    35881 atataattac ttacaagcca aatgtaaata atatatatgg ttgtcacaat ttttatcctc
    35941 aaaggggtac aggcttgcca tatgattttt ttgtaagatg ttcaaataga tttaaaaagt
    36001 atggaataag aactgctgct tttgttaatt caaaatatgc aacaatcgga ccttggaata
    36061 taaatgatgg actgtgtacg cttgaaatgc atagaaactt agatattgct acagcaacta
    36121 agcacttatt cgcaacagga ttgattgacg atgtaattgt agggaatgca tatgcaagtg
    36181 aagatgaatt aaaaagaatg tctgaagtaa ataaatatca agttgtattt aatgtagtaa
    36241 ctgaggaaaa aacttctgaa gtagaaaaga caataatggc aaatgagcaa cactttagaa
    36301 gaggggatat aaccaaccaa gtagcaaggt ctacagaagt aagaaaaaaa tatgctaaag
    36361 aaaatttccc taagcatgat aatacgacca tctttaaaag aggagatgta gtgattggta
    36421 atgatgactt tggaaagtat aaaggggaat tacagttgat acttcaagat tgtgcagatg
    36481 aaagaaaaaa taaggtaggt agaataaagg aagacgaaat aatgctttta gactttattg
    36541 aaccttggag taaatttaaa cttattttag agggagagta aagtgaagat agacatatta
    36601 ataaaaggtg gaaaaacagt agataaaagt ataattgata tagctatatt agacgataaa
    36661 ataatagaag taaaaaatca gatagatgat tctaagtatc aggcaaatca aattataaac
    36721 ctagatggag aaaaatttat tagtgctggt tggatagata tacatacgca ttgctacgaa
    36781 agactagacc tttataagga ttatccagat gaggttggaa taaaaagcgg tgttactact
    36841 gtagtagatg caggaacaac aggagcttta gatataggta aattttatga agatactaaa
    36901 aaatataaaa caaatgtata tgctttaata aatattgcta agcagggaat aacatctcaa
    36961 gatgaacttt caagtatgat gaatattgat gaatatgaac ttaaaagagc agttaaaaaa
    37021 tacaaagatt ttgttgttgg aatcaaagct agaatgagta aaagtgttgt aatttcaaat
    37081 gatgttgaac ctttaaaagt agctaaaaga ataaaaaatg aacttaatct acctatgatg
    37141 gttcactttg ggagttctcc tccaacgata gaggatatat ttgactatat ggaaaaagga
    37201 gatatattaa cccatatata taatggaaag ccaaatggaa tacttagagg caatgaggtt
    37261 aaaaaagaaa tcatagaagc tagagaaaga ggtataatcc ttgatgtagg tcatggaacc
    37321 gaaagtttta gtatggatat tgcaatgaaa tctaaggatg caggaatttt tccagacact
    37381 ataagtacag atatatatat taaaaataga ataaatggtc cagtgtataa tctatctaca
    37441 acaatggaaa agtttatata tatgggctat tcacttgaag atataataga taaagttact
    37501 aaaaatgcag cagatgcaat aagtttaaaa aataaaggtt taataaaaga aggatatgat
    37561 gctgatttaa ctatatttga tgtggttaat gaagaaaaag aacttcaaga ttctttgaat
    37621 aagtcagtaa tcacaagtac gtctataaag ccaagagcag tagtagtcaa tggagaatat
    37681 ttaaatatag gtaagtctat aggagagaat gagtagaagg gagaaactat tgtatgagta
    37741 gtatatttga aaaatactct gtaagacgtg ttataaatgc ttcaggcaaa atgactatac
    37801 taggagtatc aacagtagat aaggaagtta ttgaagcaat aggtgaaggg tgtaaaaatt
    37861 tctttataat agaagaactt ataaataaaa caggtgaata tatagcaaat ttattagggt
    37921 gtgaaagtgc actaatagtt tctagtgcat ctgctgggat tgcacagagt gtgggagcaa
    37981 tgatagcaaa agatgatatg agtctagttt acaatgtaaa taatcctaag aaacaagtta
    38041 aaagggaaat aataattcca aaggggcata atgttgatta tggagttcca gtagaagtaa
    38101 tgataagact tggaggagga gaagttgttg aggccggcta tgctaatatg tgtaacaagg
    38161 aacatataat aagagagata aatgaaaata cagcagcaat tatgtatatt aaatctcatc
    38221 attgtgttca aaagagtatg ctaagtgtat tagatgcagt acaagttgct aaagaatata
    38281 atttaccttt gatagtagat gcagcagcag aagaagattt aaatgtatat tatgaattgg
    38341 gagcagacct agtgatatac agtggtgcta aggctataga gggtcctagc tcagggttag
    38401 taataggtaa gaaaaaatac atagataatg taaagcttca atcaaagggt ataggaagag
    38461 ctatgaaaat aggaaaagaa aatattgtag gtctaacatg tgcaattgag agatatataa
    38521 gtaatgataa agtaacttta aaagaaatgg aggacaagct aaatccattt atagacaaga
    38581 taaatactat aaagggagtg agtgctagta taactagaga ttcagcaggt agagaaatac
    38641 ttcgtggaga aattgacttt aatgaagata taataaataa gtcaacacaa caaattataa
    38701 atgaacttag aagtggagag atagctatat acactagaga ctataaggct aatgaaggca
    38761 agattgagat tgatataaga agtgtaactg gaagtgattt agatactatt tataatagga
    38821 taaaaaatat agtagaaagt tagagtgtag agtaagtgac attataatga tttaataaat
    38881 taaattttac caatggaggg gataaatatg aattgtatac cacaatatta taaaaataga
    38941 gtttgtttaa atgttcttgc aggaagcata aaaaatgcta aagaagtata tgatgcttgt
    39001 gatgggtatg tattgatagg agtactatca aagaattaca gtactgtaga agcagctatt
    39061 gaagatatga aaaaatactc aaaggaaata gataatgcaa tatctgtggg gcttggagct
    39121 ggagacccaa atcagtcaaa tatggtatct cagatttcta aagaacttca gccacaacat
    39181 ataaatcaag tgtttacagg tgtggctacg tctagagcat tgcttggtca aaatgatagt
    39241 ataataaatg gattagtttc tccaactggt aaaccaggat ttgtaaaaat atccacaggt
    39301 ccattaagtt caaaagaaaa agatggtata gttcctatag aaacagcaat agctatgatg
    39361 aaagatatgg gaggaagctc aattaagttt tttccaatgg gagggcttaa aacaaaggat
    39421 gaatatatag aagttgcaaa agcttgtgca aagcataatt tttatttaga accaacaggg
    39481 ggtatagact tagataactt caaagagata gttcaaattg cacttgatgc tggtgtagaa
    39541 aaagttatac ctcatgttta tacatcaata attgataaag aaacaggtga aacgaaagtg
    39601 gaagatatag gagtacttta taaaataatg aaagaattat tgggatagat ttatgaatat
    39661 attagctttt ggtgaaataa tgatgagact ttcagtacct gactacaagt tcttaacaca
    39721 aaccaatgaa ctaaattata taattacagg cacaggatta aatatattat ctggacttaa
    39781 aaactttgga tacaatacct atatgttgac aaagttgcct aataataatg taggtaaagc
    39841 atcaagtgca aatataagaa agcttggagt aaaagatgac tttatcacat atggagggaa
    39901 tcatatagga gtgtattttc ttgaaaatgg gtatggagaa agaccttctg aagtgactta
    39961 tttgaatagg ttaaatagtt cattttgtga gagtaaatta caagattatg atattgataa
    40021 atgtttagaa gaaatggata tagtccttat ttgtggtatt gctatgcaac ttacagattc
    40081 aattaaagag attgctttag aactagctag aaaatcttat gaaaagggga taaaagtatt
    40141 ctttgatttt aattttagac aatcattaaa tccagaaagg acttatgatg atttagtaaa
    40201 tgattataag aaaatactac catattgtca tggtctattt gctagttata gagatataaa
    40261 taagatactt aatatagata gtctaaatat aaaatctagt tcagaattga ttaaaaaaga
    40321 gtatgatata ggtattgtag ctggaactat gagaagattt gatgatatga ataatagatt
    40381 tataaaggga tatatatcaa atgatgatgg ttattttgaa agtagggagt ataagttaga
    40441 aatatatgac agggttggtg ccggagatgg atatgtagca ggaattatat attcatattt
    40501 ggaaaatatg aagagtgaag atatagttga gtttggggtt gctagttcgg tgttagctca
    40561 tacaacttat ggggataatt ctttagtaag cacaggtcat gtacttagac ttatgaacaa
    40621 tgaaaaaata gatatattga gataatacat ttgtatataa taaaattttt atctttttta
    40681 gacacacttt tgacttgttt atatttgtta aacagagtta tttttaatga ttataaatat
    40741 taaattaaaa gttaaataat aaaatagtag aaaatagcat taactataga aaaaatggtt
    40801 aatgctattt ttatcgttaa tattgattaa ttttatatta tagaaatgaa cttttctaat
    40861 gctggatttg tatttttttt attccataca actacaactt caattctatt ttctacttct
    40921 tttataggta ctattgcaat atcacgttct atcattgtat ctgtaacaca aaatgaaggt
    40981 aaaatggata ttcccatgtt agaagatacc aatgattcaa tagtattaag attatttgaa
    41041 cgttgtacca cattaggtac aaaccctgct tcacgatatc tattctgtat cattgacttt
    41101 tgtccatact catcccctgt ttcttttaca aaaataaatt tatcatgttg taattcaatt
    41161 aaatcgatag aatcctcatt gtaaagtgga tgaccaggag gtaaaagagc taccaaagaa
    41221 tatctttcta cagttttata ttcaaaatct tttaatggtt gattttctgg attaataaca
    41281 aaagcgaggt ccatagaatt attttttact aagtttaaaa gttcggttat ttcattagta
    41341 caaatatcca tagatatatt agggtatgta ttagaaaatt cttttaaata ttgttgaagt
    41401 ttgttttttt catatcctat taaggttcca atgctcaaag ttccaaaaaa accatgtgca
    41461 aattgttgtg tttttgcaat agcatcattg atatttttta ttatagtacg tgcctcagat
    41521 aaaaaaactt ttccagctgg tgttagttca acatgacgtt tacttcttgt aaatagagtt
    41581 acatttattt gttcttctaa agcttttatt tgctgtgtaa cagcagtttg agaaatataa
    41641 aatttttctg ctgttttggt aaaacttaaa ttttctgcta cagatataaa atattctaat
    41701 tgtttagtat tcaaataatc acccctattt aaacttgatt gtttgtaaac cttatttatc
    41761 tataa
//
LOCUS       DACULQ010000011        41596 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.011, whole genome shotgun sequence.
ACCESSION   DACULQ010000011 DACULQ010000000
VERSION     DACULQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41596
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.011"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..198
                     /locus_tag="JA154_02580"
     CDS             <1..198
                     /locus_tag="JA154_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994742.1"
                     /translation="NDAKLLVDDYSKTFVDDIKILNIKGILYMFENKLDKADFMLKKA
                     LSLDVENEDTIYNIEYLKSLR"
     gene            222..2099
                     /locus_tag="JA154_02585"
     CDS             222..2099
                     /locus_tag="JA154_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAT7994743.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            2092..4206
                     /locus_tag="JA154_02590"
     CDS             2092..4206
                     /locus_tag="JA154_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAT7994744.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            4215..5210
                     /locus_tag="JA154_02595"
     CDS             4215..5210
                     /locus_tag="JA154_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7994745.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            5302..6534
                     /locus_tag="JA154_02600"
     CDS             5302..6534
                     /locus_tag="JA154_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAT7994746.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            6554..7498
                     /locus_tag="JA154_02605"
     CDS             6554..7498
                     /locus_tag="JA154_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HAT7994747.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            7592..7999
                     /locus_tag="JA154_02610"
     CDS             7592..7999
                     /locus_tag="JA154_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HAT7994748.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            8086..9177
                     /locus_tag="JA154_02615"
     CDS             8086..9177
                     /locus_tag="JA154_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAT7994749.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
     regulatory      9486..9576
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            9984..10301
                     /locus_tag="JA154_02620"
     CDS             9984..10301
                     /locus_tag="JA154_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HAT7994750.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            10309..10713
                     /locus_tag="JA154_02625"
     CDS             10309..10713
                     /locus_tag="JA154_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAT7994751.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            10723..11040
                     /locus_tag="JA154_02630"
     CDS             10723..11040
                     /locus_tag="JA154_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAT7994752.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            11065..12615
                     /locus_tag="JA154_02635"
     CDS             11065..12615
                     /locus_tag="JA154_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HAT7994753.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            12628..13698
                     /locus_tag="JA154_02640"
     CDS             12628..13698
                     /locus_tag="JA154_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAT7994754.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            13691..14422
                     /locus_tag="JA154_02645"
     CDS             13691..14422
                     /locus_tag="JA154_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAT7994755.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            14426..15745
                     /locus_tag="JA154_02650"
     CDS             14426..15745
                     /locus_tag="JA154_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HAT7994756.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            15782..16222
                     /locus_tag="JA154_02655"
     CDS             15782..16222
                     /locus_tag="JA154_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HAT7994757.1"
                     /translation="MDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLENL
                     ESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLISKQ
                     IERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            16251..17456
                     /locus_tag="JA154_02660"
     CDS             16251..17456
                     /locus_tag="JA154_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HAT7994758.1"
                     /translation="MKIQADSYNIIKDIGDRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            17474..18082
                     /locus_tag="JA154_02665"
     CDS             17474..18082
                     /locus_tag="JA154_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HAT7994759.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            18109..19092
                     /locus_tag="JA154_02670"
     CDS             18109..19092
                     /locus_tag="JA154_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgC"
                     /protein_id="HAT7994760.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            19105..19308
                     /locus_tag="JA154_02675"
     CDS             19105..19308
                     /locus_tag="JA154_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbD"
                     /protein_id="HAT7994761.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            19308..20126
                     /locus_tag="JA154_02680"
     CDS             19308..20126
                     /locus_tag="JA154_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086725.1"
                     /note="Homolog of MotA, appears to be involved in motility
                     on surfaces and under different ionic conditions. With
                     MotS (a MotB homolog) forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HAT7994762.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            20116..20811
                     /locus_tag="JA154_02685"
     CDS             20116..20811
                     /locus_tag="JA154_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein MotB"
                     /protein_id="HAT7994763.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            20823..21266
                     /locus_tag="JA154_02690"
     CDS             20823..21266
                     /locus_tag="JA154_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FliL"
                     /protein_id="HAT7994764.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIADLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            21281..21655
                     /locus_tag="JA154_02695"
     CDS             21281..21655
                     /locus_tag="JA154_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HAT7994765.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            21656..22321
                     /locus_tag="JA154_02700"
     CDS             21656..22321
                     /locus_tag="JA154_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HAT7994766.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            22334..22603
                     /locus_tag="JA154_02705"
     CDS             22334..22603
                     /locus_tag="JA154_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HAT7994767.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            22615..24429
                     /locus_tag="JA154_02710"
     CDS             22615..24429
                     /locus_tag="JA154_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086731.1"
                     /note="FlhB is a membrane protein responsible for
                     substrate specificity in switching from rod/hook-type
                     export to filament-type export; FliR is required for
                     export of flagellar proteins, although a specific role has
                     not yet been determined; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HAT7994768.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNNIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            24438..26513
                     /gene="flhA"
                     /locus_tag="JA154_02715"
     CDS             24438..26513
                     /gene="flhA"
                     /locus_tag="JA154_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086732.1"
                     /note="membrane protein involved in the flagellar export
                     apparatus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HAT7994769.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGDLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            26488..27504
                     /locus_tag="JA154_02720"
     CDS             26488..27504
                     /locus_tag="JA154_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HAT7994770.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            27501..28379
                     /locus_tag="JA154_02725"
     CDS             27501..28379
                     /locus_tag="JA154_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HAT7994771.1"
                     /translation="MIDQAQILRKIAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            28393..29094
                     /locus_tag="JA154_02730"
     CDS             28393..29094
                     /locus_tag="JA154_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HAT7994772.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            29110..29532
                     /locus_tag="JA154_02735"
     CDS             29110..29532
                     /locus_tag="JA154_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HAT7994773.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            29517..29702
                     /locus_tag="JA154_02740"
     CDS             29517..29702
                     /locus_tag="JA154_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HAT7994774.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            29743..30516
                     /locus_tag="JA154_02745"
     CDS             29743..30516
                     /locus_tag="JA154_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgG"
                     /protein_id="HAT7994775.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            30537..31298
                     /locus_tag="JA154_02750"
     CDS             30537..31298
                     /locus_tag="JA154_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgG"
                     /protein_id="HAT7994776.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            31321..32202
                     /locus_tag="JA154_02755"
     CDS             31321..32202
                     /locus_tag="JA154_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAT7994777.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            32205..32579
                     /locus_tag="JA154_02760"
     CDS             32205..32579
                     /locus_tag="JA154_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAT7994778.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            32611..34014
                     /locus_tag="JA154_02765"
     CDS             32611..34014
                     /locus_tag="JA154_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994779.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLYMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            34130..36067
                     /locus_tag="JA154_02770"
     CDS             34130..36067
                     /locus_tag="JA154_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAT7994780.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            36454..37584
                     /locus_tag="JA154_02775"
     CDS             36454..37584
                     /locus_tag="JA154_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAT7994781.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            37811..38833
                     /locus_tag="JA154_02780"
     CDS             37811..38833
                     /locus_tag="JA154_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HAT7994782.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            39015..39926
                     /locus_tag="JA154_02785"
     CDS             39015..39926
                     /locus_tag="JA154_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7994783.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            39941..40357
                     /locus_tag="JA154_02790"
     CDS             39941..40357
                     /locus_tag="JA154_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994784.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(40457..40849)
                     /locus_tag="JA154_02795"
     CDS             complement(40457..40849)
                     /locus_tag="JA154_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7994785.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            40990..41385
                     /locus_tag="JA154_02800"
     CDS             40990..41385
                     /locus_tag="JA154_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5-phosphate oxidase"
                     /protein_id="HAT7994786.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        16997 a         4243 c         7550 g        12806 t
ORIGIN      
        1 aatgatgcga aattattggt agatgactat tcaaaaacat ttgtggatga tataaagatt
       61 ttaaatataa agggaatttt atacatgttt gaaaataagc ttgataaagc tgattttatg
      121 ttaaaaaagg cattatcatt agatgtagaa aatgaagata ctatatataa tatagaatac
      181 ttaaaaagtt taagataagt caaaaatagg agaattgaag aatgaatata ccaatagtgg
      241 ttaatgaaaa atctaaagac ataataaaag attggcaata tacatgtaca aatgaagata
      301 taaaatttac aagcataata atattgacat ataatcaaat agaatatact aaattatgta
      361 ttgaaagtat taggaaattt accccaaaga ataaatatga aataataata attgataata
      421 actcttctga tggaacagtt gagtggttaa agggacaatc tgatataaaa tctgtgtata
      481 ataacaaaaa cttaggattt ccaaaaggat gcaatcaagg tattgagtta gctactggtg
      541 aaaacatatt gttgttaaat aatgatgtaa tagttactcc aaattggctc tataatttga
      601 ataaagcttt atggtctcaa gataatatag gtgctgtagg acctattacg aatgcatgtt
      661 catattatca gcaaattgat gttgattata agtcaattga ggacatgttt atatttgcac
      721 acaaaataaa taaatctaat attaacctat ggaaatatag attgaagtta gttggatttt
      781 gcatgttaat aaaaaaaact gttctggata atgtaggggt tttagatgaa ttattcacac
      841 ctggaaattt tgaagatgat gatttatctt ttaggatgat agagtcagga tataagttat
      901 tattatgcca ggatactttt atacaccatt ttggaagtgt gtcttttaaa gaaaatcgta
      961 ataaatatac agatataatg tatattaata gaagaaaatt tgaaactaag tggggattta
     1021 atccagaata tagtggaatg ataagaacag atttgatttc taaaatagat gatagtttgg
     1081 aggcaaatat taatgtttta gaaataggat gcggtatagg ggctactctt ttagaaataa
     1141 aaaatatata taaaaatgca tcaatatatg gaatagaaat tagtgagtca gcaggtaaac
     1201 ttgcaaagta tagtgcagat gtacttatag caaatataga aaaagtaaaa ttggattata
     1261 aaaaagattt ttttgattat ataatattgg gagatgtact tgaacatttg attgacccat
     1321 ggaagatagt caatgaattg aaacaatatc taaagaagga tggttatctg gtatctagta
     1381 taccaaatat tatgcatgtt tctgttttaa gaaatcttgt gttaggtaat tttacatatg
     1441 aagattcagg aattttggac aagactcatt taaggttttt tacattaaat gagataacaa
     1501 aaatgtttaa tgatagtaat tacataatta atagtattac agctactagt atgccacttt
     1561 cggaaattga taatagcttc attgatacaa tatgtaagga aagctgtaaa gaaatgagac
     1621 aacagtacga atcttatcaa tatatagtaa aagcctctaa aatcctagat actaaaagat
     1681 attctagtat tagtatgatt aatttaaaat acgctttaat gagaattgat aatgaattag
     1741 atgtagatag taatttaagt tatatatttg ataattataa tataactgaa ccatgttttg
     1801 ttgatgatat tgaatatcta attgatacat ttgtgataaa taaagcaact ctattaaata
     1861 gattaggagt tgaggcattt aacagaggtc taggagattt tagtataaac atgtttatga
     1921 cagtattaga gatggataga gatgatattg actcaatata taatataaca agtgtattat
     1981 gtgaattaga agaatttgat actgcttata atttaattaa aaatagtagc gaaaaagtac
     2041 agagtgaaag tgatatacaa gagatacttt gttttattga ggggaagaaa tatgaataat
     2101 gataagatat gttttataac ctgtgtaaat gatgaaaggc tttatgaaga atgtcttgta
     2161 tatataaata gcctagacat tcctaaaaac tttagtatag aaacagtagc tttaagagga
     2221 gccgattcta ttgcctctgc ttataataaa gctatgagag aaacagattc taagtataag
     2281 gtatacttac atcaagatac ttttataatt aataaaaatt tcatacatga tattttagag
     2341 atatttaaaa aaaatacaga tgtcggaatg attggtgtag ctggagtaaa agatatgcct
     2401 gtgaatggaa tttggtggga ttctgataaa aaagcaggga aagtatatga tagtcataca
     2461 ggtaaaatgg atatttatga atttagtaac tttgaagaat gttttaatga agttatggct
     2521 atagatggat taattatgat tactcaatat gatgtatttt ggagagagga taagtttgat
     2581 gggtggcatt tttatgattt gtcacagagt atggagttta gacaaaataa atacaaagtt
     2641 attgtaccaa aacaaaataa gccttggtgt atgcatgatt ctggagtagt aaatataaat
     2701 aatggatatg atgaatatag gataaaattt gtaaaagagt acataaatct tgatttatat
     2761 agagaaaatt tttatgaatt tgataagaag aaagcaagta ccatacttaa gagtaataag
     2821 catgtaatgg attgtatgaa agaggagctt acagaacaat taagattaaa gaattacaag
     2881 gatgttttag gtattgtttc taaaattgct catttttctg tttttaatca tactggtttg
     2941 tattcttgtg aagaatgtga gagagcattg ctagaatgtg caaataattt accagaaata
     3001 gaaaatacga aattatttac aaaaaaagat ttaaaaagaa gaaaagtatt acatgtttta
     3061 agtgaaggat attcaacagg aggtcataca agactagtta aaaattggat tgaaattgac
     3121 aaagactctg ttcattcagt tgtaactaca tggcaatcag atacactgcc agactggtta
     3181 ttaaaagcaa taaatgatag tggtggcgaa gttatatctt tggatttaat ttcagatgat
     3241 tatttaaaaa gagctgtagc tttgaggaaa atagcttatg actgggctga tatagttatt
     3301 ttatatgttc atatgtggga tacaatacct gttgttgctt ttggagttga tgggaaaata
     3361 cctattttac atgttaatca ctatgatagt actttttggt tagggacaag tattgcagat
     3421 attgtcattg attttagcga aactagtcaa ttaattacat tagataaaag aggcatagat
     3481 aaatctgaaa tattaccaat gccattaaaa gaaaaagaag tattttcaaa atctgaagtt
     3541 agaaaacgat ataatttaga ggatgatgtg acaataatat tgactattgc tagtgaaaga
     3601 aaatacaata gtgtaaatga tattaattac tttaaaattg ctaaaacatt gattgaaaat
     3661 acagataata caattattat tgttgtagga gttgatacag agaatctcta ttggaaaact
     3721 ttaagtaatg ttacagataa tagagttata tgttttggaa caacagcaaa tatagatgat
     3781 ttttataaga tttcggatat atacctagag tctttcatgg taggaagtca tacttcaaaa
     3841 ttagatgcaa ttttatatga tttaattcct gttaagttta aaaatgaagt ttcaccaatt
     3901 tttacaaata tgtggaattt tatagataag tttgattata agaatataaa agaggttata
     3961 gacatgataa gaaggtacaa tactagtgaa tttgacaggg ataagataaa agcaaaacaa
     4021 tcggaaataa aacattttgt tatagataaa cattgtattg aaataaaaaa ctatatagac
     4081 aaaatatata atgaagttaa tcatcataaa gttaagtatg atttatataa aaatgatgaa
     4141 atcgaggact atcagctttt tgtagcatta tatggctata tggtaaaaaa taaaaacatg
     4201 tcataggtgg taaaatgaac aatattcata tcaataaatt tgaaaaaagc tgtgaaaaaa
     4261 actgggatta ttttgtagaa aataaatcta taaatggtac atttctgcaa actagaaatt
     4321 tccttaatta tcatgaggag ggaaaattca ctgaccactc attaataata tgtaaaggtg
     4381 aagaaatact agcagtcgta cctgcatgtg aaacttatga agatggtgaa agaatatttt
     4441 tttctcattt agggagtact tttggtggca ttataataag aagtggtttt aatgatatta
     4501 ggcatgtaaa tgccattaca aataagttag aaatatattt acaggaacaa aatttcgata
     4561 aagtatattt aaaaaataca tcaagaatat tttgtaaaga ggatacttca ttactagaat
     4621 attttttatt taagaataat tacatgtttt atgatgaatt aagtatgttt ttagaattaa
     4681 gtaagtataa agaagatata ttagccaatt ttaaatacag taaaagaaga gattttaaat
     4741 attctttgaa gaataatctt acctttaaaa gactgtatag tgatgaagat atagtttgtt
     4801 tttttaattt actttctaat tgtttagata gtaaaggagc taaagtaata cattcagtag
     4861 aagaactttt ggagtttaaa aataatagat taaaagatat tgtagaattt tatgcagtat
     4921 atattgatga cattatgatt tgtgggagca tggtatttaa ctttcaagaa agagtttatc
     4981 atactcaata tctagcatca aattcgacat ataataaata ttatccaatg atatttttat
     5041 actataatct aataaaagat gctttagaga gaaattttaa gtatctttct tttggaatta
     5101 gcactctaaa tagaggcaaa tacttagatg agggtttagc tacatttaaa gaaggttttg
     5161 gttcaagtgc aatttgtaac agaacatact ataaaaatat aaataagtga gaatcatgtt
     5221 ttatagagtt tttagagtat agtaaatatc tagtactata atattacata aaaataatta
     5281 gtttataaag aaggtttgta tatgaaaaga ttaatgattt taggctcaat taagtatttt
     5341 gagaatattg ttatgtcagc taaaagagag ggaatataca caattgtttg tgataataga
     5401 attgatactc ctgcgaaaaa gatttgtgat gaagcaatag atgttgatgt atttgatttt
     5461 gataaaataa agaatatagc tatagataaa aagatagatg gaattttaac tggatttaca
     5521 gattctttaa tgaagccata tgtttatatt gcaaatgaac taaatttgcc atgtgtgatt
     5581 tcttgcaatc aacttgaatc tgtaactaat aaggctgtta tgaaaaatta ttttaaaaat
     5641 aatggaatac ctacaactga ttattgtatt attgaaagtt tgaaagattt agaaaaaata
     5701 caaggattaa attttccact tgtaataaaa cctgttgatg gttatggttc aagaggtgta
     5761 tattttgtat caaacataga agaattaata gaaaaatttg aatattctaa ggaagcctcg
     5821 caagatggac aagtaataat tgaggaattt tatgaaagtg aggaaataca aggactagct
     5881 tgggttcata atggagagtc acatgtattt tatattggag atagagagct tgtaaatata
     5941 catacaggaa gagctggaaa accagataga ttaatttatc catcaaagta ttgttttcaa
     6001 tatgaagaag aaataaaatc catatatcaa aatataacgc aggcttttgg cataaaaaat
     6061 ggacctcttt atgtacaaat gttagtggga gcatatggaa taaaggtatc tgaagtaatg
     6121 ccaagattac ctggaggttg tgactatatg gctatttctc aaataacaga tttagacata
     6181 ggaagtttat ttgcaaactt tagtgtgaat aatgaagtaa attttgatga aataaagaaa
     6241 tataatatgc atctatcaaa atgtgtatat gcattaccaa tatacataag acctggcaca
     6301 ataaaaaaga taaataatat agataaaata gaaaacttag attatgtcac tcaatttcta
     6361 ttactagtaa aagaaggaga tacaatagga ttatatgaag atgagagaca ggattgtgga
     6421 agagtttatg gagtagcaaa taatgtattt gatgcaaata taaagaagaa atgtattcat
     6481 gaaatgatag aaatacttga tgaaaataat gaaaatatga tagaaaattt ttaaataagg
     6541 gagtatgata gatatgaagc taataactca tgaacaaatt atgtcattaa acatttctca
     6601 gaaacaagga tacgagtgga taaaagaatc ctttttaaca aaaaaagatt gtcaattacc
     6661 accaaaaata agcatggtag aagaagggca tgtattttac aatgtaatgc cgtccctaaa
     6721 tcataatcat aagattggtg gaataaaaat ggttacaaga ttgccaggga gaaatccaag
     6781 tttaaattca gagatatttc tatataatat ggaagatgct aaattaaagg ctattttaga
     6841 tggcaattat attacagctt ttaggactgc tgctgttgct gccttggcta tcgatacatt
     6901 tgctaaaaaa gattttaata ctattgctat tatgggttta ggtgtaactg cttctgctat
     6961 aatgaatatg ctttcatata ctcttaaaga taaaaatata aatataaagt tacttaaata
     7021 taaagaccaa gcagagtcgt ttgtaaaaaa gtatgaaaaa aatgaacaat ttagttttga
     7081 aatatgtgac acacatgaag aacttattgt agattcagat gtagttattt ctactgtgac
     7141 atatacagat gaatatttat cagaatttaa atggtttaaa aaaggtgctt tattattacc
     7201 tgtacatgtt atgggatttg gaaatgttga ttatctattt gataaagtat atttggatga
     7261 ttataatcat ttgaaacatc tagaaaatat agatagatat aaatattctt gtgaatttta
     7321 tgatgtttta agtaaacaag ctaaaggaag agaaaatgat gatgagagaa taatatctta
     7381 caatataggt ttgtcaatac atgatatatt atttgcaaat aaaatttacg aattgtgtga
     7441 aacactaagc tgtgaagaaa tagaattaaa agaaccaaaa gaaaaatatt ggatatagtt
     7501 tatttcattc atatttacaa tagtttattg taaatatgaa tatgaagtaa tatattgtta
     7561 gaaaattagc atgaatagga tagataaatt tatggataaa tataatttaa ttaggttcgt
     7621 atctattgga aacgaatata taggaaattt agttgcatta gaatcaaata aagcaatacc
     7681 ttttgatata aagagagttt attacatata tgatgtacca aacaatgtaa agagaggatt
     7741 tcatgcacac aaaaacttgg aacaagtact agtttgccta aatggaagtg tgaagataaa
     7801 atgttatgat ggtgtaaaag agactatatt taatttacat aaaaatgatg ttggattata
     7861 tataggaacg ggagtgtggc atgagatgtt tgattttgat gaaaaaactg ttttaatttc
     7921 tatagcatct gaacattata atgaagaaga ttatattaga gactataaag aatttttaaa
     7981 gtatttaaat aatgtgtagt tattatacaa ttagtgatgt attttcttaa ttaaataaat
     8041 ttaagatagc cacatagaga atagatagat taggatgtga atttattgtt ggttaaattt
     8101 aataatttat taaaacagtt ccaaactcat aaagaagaat atgaacagaa gattaaggat
     8161 gtaatggaaa gtggatatta tatccttgga aatgaagtaa agcattttga agaagaattt
     8221 gctaactatg taggtagcaa atattgtgtt ggtttagcca gtggtttaga tgcactgata
     8281 attgctttta gagcattagg aataagcaaa ggtgatgaag taatcgtgca atcaaataca
     8341 tatattgcta gtgttatggg aatcagtata aatggagcaa caccagtatt tgtagaacca
     8401 aatgaatatt ataatataga ttcatctaag atagaagaaa agataaatag caaaacaaaa
     8461 gctatattag tagtgcattt atatggtcaa gcaagtaata tgaaagatat acaagaatta
     8521 tgtgataagt ataacttgta tttaattgaa gattgtgctc aatctcatgg agctaaatat
     8581 gatggaaaaa tgacaggaac atttggtgat ataggttgtt ttagttttta tccaagtaaa
     8641 aatttgggtg catttggaga tgcaggagct atagtaacta gtaatgagga aatagctgat
     8701 aaagtgaaaa ttcttagaaa ctatggctca gaaaagcgat attataataa atttgtcgga
     8761 tataattcaa gattggacga aattcaggca ggtttattaa gagtaaaact taaatatcta
     8821 aatgaaattg aaattgaaaa atcaaaaatc gtagatagat atcttaatga aattaataat
     8881 cccaaagtca tacttccaaa gatagaaaat aattgtactc atgtatggca tttatttgtt
     8941 ttacaagttg aaaaacgaga tacatttatg gaatacttaa agaaaagtgg tatagagtct
     9001 ataatacatt atccgatacc accacatcta tctgaagcat atgcttattt gggatataaa
     9061 aaggggagtt ttaatatagc tgagaactat tcagataaag ttgttagtat acctttattt
     9121 attggtatgg aagatgaaga agttagttat ataattaaaa ttataaatga atattaaact
     9181 actcataata atatcaaaaa taaatttttt atatatcatt tatttaaatt tcgttaattg
     9241 acatttttaa atattgatat attgtacaaa taaaattgaa atatatggaa aaatatagaa
     9301 ttaagatgag atttgtctta tatttttata aattaaaaat attttcaaag agtttattat
     9361 aaaattgtaa actatagtgt ttataatctt tgtaattatt ggtaattaaa tttaaaaaat
     9421 aaaatattta attatacaca aaaaagatat ttaaaaaatt tgttatttat tgtatactta
     9481 agttaaaact aaataggcaa atctagagaa atctagtgac gcaaagctat agggactaag
     9541 gtttatatac ataaactatg tcagccagtt gccaaaaaga gtcctaggtg tattgtatac
     9601 ctaagaaaag tctataatga ctgccttttt ggcagtcatt ttgttatgga aaaatcactt
     9661 tatttcagta gaaaatacac ataataacat attttaccaa ttataacata aaataaagaa
     9721 aaattatctc aaaaagttac tattttacaa aaaatgactt ctttatttat ttaaattaat
     9781 cgcatttcca ttacattaat acaataatgt atcaatttag taagaaaact aatatttttt
     9841 taatgaagtc ccatgaaaat ataaaaaaat tttaatatgt atttaaatat ttcttcgaaa
     9901 agttgctctt tttgtaatat agcaacttca ttttttttat taataaacaa aatagataaa
     9961 atatattttg aggaggggtg agtgtgaata catatgacat tataaaaatg ggattagatg
    10021 caactaatct aagaagtcag acaatagcaa ataatatagc aaatataaat actgaaggat
    10081 ataaaagaca ttatataact tttgaagaaa cactaaatga tgctatgcct aaaatagaag
    10141 aaaaaacaga caattctaaa ggtttaagag tagatggtaa taatgtagac atagaagaag
    10201 aaaaagtaaa tcaagcaatg gcaacattac aatataatgc tttgataagt tttgcaaatg
    10261 gaaaaatagc aatgaccaaa tcaataataa gtgggaggta attagaagat gagtgtgttc
    10321 agtggaatga gaatatctgc tagtggatta acagcagaaa gtttgaaaat ggatatcata
    10381 tcatccaatg tagctaatat aaatgctaca agaggtccaa atggagaagc atataggaga
    10441 aaagttgttt catttgaaga aaactatgat aataaattag gtatgctagg agtaaaagtt
    10501 acatctgttg aagatgataa atcacctctt aaagaagtat atgacccatc tcatccagat
    10561 gcaaagccaa atggatatgt agaatatcca aatgtaaata tattggtaga aatgacagat
    10621 ttaataacag cttcacgagc atatgattct aacgtagata cattgaatgc tcagaagtct
    10681 atgatatcaa aagctttgga aataggaaag tagggataaa ttatgccaat tacacaaatt
    10741 aacaatttaa tgcaaggtgc aaagttagat aatttgattg gagaagcaaa gccattaact
    10801 cttaataata aagaagaaaa aaagaatttt gaagatgtaa taatagatac tatagataaa
    10861 gttaattttc aacaagtaac agcagatgat atgaaagaaa aatttataaa aggcgaagat
    10921 gtttctatgc atgaagttat gttaataggt caagaagcac aactatctat gcaatattta
    10981 atagaagtta gaaataagat tcatgatgca tatcaagaaa tgaataaggt gcaaatttag
    11041 ccttttgaac aggagtaata atcaatgata accagtttta aaaataagat taaggagcaa
    11101 gttgctaaat ctaggacaaa gataagttct ttaaaaaagg gaactaaaat agcattaggt
    11161 ataggggtta ttgctatatg tttagcgcta gtatttacag caatatctgc gagcaaaaat
    11221 aagtatgcag tcttattttc agaactagat gctcaagatg ctgcagatat atcagcagag
    11281 ctagagtcta aaaagataga tactaaggta aaaggaagta cgatttatgt acctagaaca
    11341 gaagttgata agttaaggct ggagttagcg ccaagtctta caggtggttc taaaggattt
    11401 gcactaatgg atgatagttc atcaataggg cttactgatg aagaatttca gattaagaaa
    11461 caaagaatga tacaaggtga aatagaaaaa acaataaaga gttttccaca agtggaagac
    11521 actagagtac atataacatt tggagagtca tcagtatttg agaaggaggc tgttccaggg
    11581 agtgcagcag tttatttgat gctaaaacca gggacgactt tagaaaaaaa ccaaataaag
    11641 tctgttatgg cacttatatc aggaagcgtt acaaatatac cagagaaaaa tgttcaggtt
    11701 attgataaca aaatgaattt attatctgaa ggtattttgg aggatgaaaa tgttgatgaa
    11761 aataatcaac caataaatag tggcattagt gttgataaaa ataaggcatc tgaacaagca
    11821 ttgaataaac aactagaaaa gtctatttta gaaatgctag aaccgatatt tggtaaggga
    11881 aaggtgaaag ctacagtaaa tgctgattta agttttgata cagctgaaaa aactgagata
    11941 aaaattgacc ctgataaagt tgcaataaag gagacaaggt cgaaaaattc atctaaatct
    12001 gctgcaaata atgttcaagg tgtagatgct aacatgaata accaaggaaa taacaatggt
    12061 actaatacag aagatagttt agatgagagt tttgagtatg aaacaggtaa gattgaaact
    12121 cacactatag ttgcgcctgg agaacttaat agattgacag cttctgttgc aatagatgga
    12181 aaaatagcta aaggtgttca agctgatgta gaggatattg ttaataatgc tataggtatg
    12241 gatggagcga ggggagatac tcttagcgtt gtttcaatgg tatttgaccc agaaggtaag
    12301 aaagaggcta aacaagagct ggaagagatt aaaaaagaag gatttaaaaa gaacataata
    12361 aaagcagtta ttggagtagt agcagtaata gcagttggag ctattggata cttaatatat
    12421 aagaaaagaa gtgaacgaga agatatggat tatgaagatg aagattatat ttctaaatct
    12481 gattctgttg ataggaagga taatcctcta ttaggagatg atccagaact gacattagag
    12541 gaagctgtta aaatgtatgc agaagaaaag ccagaccaag taacagagat aataaagact
    12601 tggttaaatg agtaaggggg atagaaagtg tccacggaaa ttacaaatgt taatgcgttt
    12661 gacttaagtg atataaatga aagtgataag aatgagaagg tagaaggtga gggtttaaaa
    12721 aaagctgctg tattattaat gacattaggc ccaaatgtgt ctagtgatat aataaaaagc
    12781 ttgcctgata aacaagttca aaaaataggt ttggaaattg caaatatttc ttcaatagct
    12841 tcaaaagaaa gaagagaaat cttaaaagag tttgtagaaa taaatagggc aaaagattat
    12901 gttatagaag gtgggcttga atatgcaaga gagttattaa gtaatgcttt aggaaatgtt
    12961 aaagcaaaca aattacttga aggtatatca ataaatacaa gtacaaagcc ttttaaacat
    13021 attataaaac tagatgtaaa gaagatatta gaaatactta ggaatgaaag tgcacaaact
    13081 atagccgttt tacttgcaca tatggaacca gaaaatggag cagaagtttt atcatactta
    13141 gattttgatt tacaaaatga agtagctatg agaataagct caatgggttc aatatctcca
    13201 gaagtaataa aggtaattga aagttcatta gaagataaat tacattcaat aagtggtgat
    13261 gattttaatg attatggagg ggtgtctacc ttagttcaaa tattgattaa ttctgataga
    13321 aaaacagaaa agaatatttt gggaactatt gaagaacaaa acccggattt agcagctcaa
    13381 attaaatctc atatatttgt ctttgaagat attgtcaagt tagatgatat aagcatacaa
    13441 aaagtgttaa aagaagtgac tatgaaagat ttagtatatg ccttaaaagg tgtagatgat
    13501 gagatttcag atgctatata tagaaatcaa tcttctagag cagcagatgc acttaaagaa
    13561 gaaatggaaa tggttggtgt tataaaggtt acacaagttg aggcttctca acaaaagata
    13621 gctaatatcg taagaaaact tgaaagagaa ggtgccataa ctattgtaag gaattttggt
    13681 ggtgactttg ttgaataata agattattaa aagtaaaaaa gcattatata aaggagtgct
    13741 agatttaacc ataatagata aagttagagt gggagaatca aatgtaaata aagaaaatga
    13801 ttgtagattt agggatgagg taataaaaga agctatagaa tctgctgaga taaaaaagaa
    13861 tgagatatta gaagaagcaa aaataaatgc acaaaatata gaaaagcaag catatgaaaa
    13921 agggtatagt caaggtcaaa aaaatggata tgaggatgga tataaagagt catatgatga
    13981 atatacccaa aaagctaaag atgaagcaga ttttataaaa aatcaggcaa atttaatctt
    14041 attacaagct aatgagaaga tgactgagta cataaaagaa aaaaataaag agattgtaag
    14101 acttgctatt aatatggcca gttctgtttt aaaaaagcat ttcgaagatg aagaatctat
    14161 gagtgatctg ttaaagcaaa ttattcaaga ttatgaagga agtagttcat ttatagtaag
    14221 atgtaattct ttctataaag ataatataga gaaagagttt gaattattca aagaggaaac
    14281 atcactaaaa tcaagtgtat ttgttattgc agatgatagt atagataaag gcaatgctat
    14341 tatccaaaag gaaaatggaa gaataatagt tggtattgat tgtgctatgg aaaaactaaa
    14401 agaagaattg cttggtgaat aaagtatgta taaaactgat atatctatag attttgacaa
    14461 aataaataag aaaatagaaa atttagaact cactatatca gaaggtagag taaagaagat
    14521 aataggactt actgtagaag ttgaaggaat aaaagctttt gttggggagt tatgcgttat
    14581 atacaaccaa gttaataaac cagtgaattg tgaagtagta ggttttaaag ataaagaagt
    14641 catattaatg gcgttgggag aattaacatt gatagcacct ggttgtaaag ttatttctaa
    14701 ggggatacct ttaagtgtta tgtgtagtga taatttacta ggtaaagtac ttgatggtct
    14761 tggaaatcct atagacaact cagatgctgt tttaggagat agatacaacc tgaacaatga
    14821 acctcctgat ccaatgaaga gaaaaaagat acgaaatata atggaaacag gtgtaagagc
    14881 aatagatgca tttactactt gtggtgaagg acagagaatc ggcatatttg caggaagtgg
    14941 agtaggtaag agtacgactc taggtatgat tgctaggaat gcaaaagcag atgtaaatgt
    15001 aattgctctt attggagaaa gaggaagaga agtactagat tttattgata aagatttagg
    15061 tgaagaggga atgaaaaaat ctgttgttgt gtgtgcaaca tcagataaag cccctctagt
    15121 taggttaaaa ggtgctctta ctgctactgc aatagcagaa tattttagag accaaggcaa
    15181 aaaagtaata cttatgatgg attcagtaac tagatttgct atggcacaaa gagaagttgg
    15241 tgtagcaata ggagagccac cagctcaaaa aggatataca ccatctgtat ttgcaatgtt
    15301 gccaaaactt atggagagaa cagggacatc agataaaggt tctataactg cattttatac
    15361 agtattagtt gatggagatg actttaatga gccaatagca gatacaacaa gagggatact
    15421 tgatggtcat atagttttat ctagagactt ggcaaataag aatcactatc cttctataga
    15481 tgtattaaac agtttaagta ggcttatgaa tgaaattgct tcaaaagaag acataaaaat
    15541 agcgtctttt gcaagagaca tgcttgctga gtatagagaa gcagaagatt tgataaatat
    15601 aggtgcatat gcttcaggta caaataaaaa aatagatgaa gcaatctatt atcatgagca
    15661 tattattaat tttttaaaac aagggataaa tgaaaagagt tcttttaatg aaactataag
    15721 tagtttgaga agagtttttg aataaacaat taaaaaaata tttgaatagg ttgtgatgga
    15781 attggataaa aactttaagt ttagattaca gaaagtttta gacttaaaag tgaaagatga
    15841 agaagagata aaaatggaat ttgctaagat tcaacaaaaa aaaatagata tagagactaa
    15901 tttagaaaac ttagaaagta attatagcaa atattctata agtaaaaata acgatagtat
    15961 tcaaaatcaa aaaataacta ttaactattt actagcttta aacaattcta taatggattt
    16021 atcagaagag ttggataagt caacaaatga acttgaaaaa gctagaaagc agcttataag
    16081 caaacagata gagagaaaat cattagaaaa attaaaagaa aaaaagtatg gacagtatta
    16141 taaagaagaa aatttaaaag aacaaaacac taatgatgag tttgcaagta tgagttattt
    16201 aaggaataga caagttctat aagaatagta ggaagtgagg atattattgt atgaagatac
    16261 aagcagattc ttataatatt attaaagata ttggggatag aaaaagttca tctaagaaca
    16321 aaaatataaa aaataataaa tttgaagata atctatataa ggcaacagac cataaaaaag
    16381 ttaaagatag aagggaagta agtaagcaaa atattagctc agataaaccg gaaaatagtg
    16441 ataatgagtg tcatttagat gaagataaaa gtaatcactt agaagatgaa aatacatatg
    16501 aaaaagattg tagtaagaat aatgaagctt tgcagcaatt actgaatctt ttaaaggata
    16561 attataaaaa agataaagat ataaacacta tattgaataa tatagaaaaa ctaagtatat
    16621 cagatgaatt aaaacaagaa cttaagctta ataaaaatat attaaataag attgcaaatc
    16681 aaaattcaga gctaaataaa gctaatataa ataactctaa tgatatagaa aatttagaca
    16741 tagttaaatt aagagaactt ttggaagata atgctaataa agatattgaa gtaattacta
    16801 aaactacaga aaatgatacc aaaaatatag atttgtatag ttttgatagc aatagaatgg
    16861 ataatcttaa ctcaaataaa cagaaagatg atagctctga tattttggaa aaactagcag
    16921 gggtaaatgg aaataagagt gggaattttg gacaagtaat aaataaaaat gttgatatga
    16981 ataaaattaa aaatgataat gtcaatttaa aacagataga atcaaatgta atggatgact
    17041 ctataaaagc tattaagcat atgaaaacaa atgatataca agaattgaca attaaattaa
    17101 gaccaaaaga attaggagat atgaatatac aattgttaaa agatggtgaa agtatgagag
    17161 ctgttgtgac tgttttcaat aaagatgtct ttgactctat aaataaaaac atagcagatt
    17221 taaagcaaca tcttgaactt actaatgtaa atataaaaga tgtatcagtg caaatgcaca
    17281 gcgataatac aaatacatct gatacctttg ataaagcatt tgaacaacag aatagacaaa
    17341 ataaccaaga aaacatgagt aagaagaata caaatgataa gcataataat gtagtcatag
    17401 aagatgacat taaagaaaat actattgatg atgatagagt agatttacta gcttagtaag
    17461 ggggaagtat tgcatgtacg gaatagcaac ggctttggca ggaactcagg gaaactatag
    17521 gtcaaataac ttaagccaac ttgaaagaaa tattaatgat attgccaata aacaagttag
    17581 aagcaaagga aatcagacag taacagacaa tgggactcca attataggac aaggtgatga
    17641 tgctgataaa gacttgttta tgaagatact tgtggcacag atgggtaatc aagacccaat
    17701 gaatccgcaa gacccaacag aatatgtgtc acaattagca cagtttgcaa ctttagaaaa
    17761 gatgtcagct atgaatgaga agctagatgt acttttagcc ttaagtaatt ccactctaat
    17821 aaactctgct ttaagtatgg caacatcatt aattggtaaa gaagtagaag tatctgggga
    17881 agagaaagaa gatgaaaaca ttgtaggagt agtaaagtcc acatatataa aagatgggga
    17941 agtacactta gaattagaag tagatggaag tgacaaaact gtaggtgtta aatatgaaaa
    18001 attacttaaa ataggagagg tagatgaaaa ttcaggagaa gaaaatgatt cttcagaaca
    18061 cgaatctcca gaagaagcat aatattaaat gagaaaagag gtaaattcat gataaaagca
    18121 atgtattcag gtgtaagtgg aatgaaagca aatcaaacaa aattagatgt aataggtaac
    18181 aatgtagcca acgctggaac tacttctttt aagaaaagtt cagctagaat aacagattca
    18241 ttttatcaaa ctgtactata tgcaagtgct ccaacagctg cattaggagg aacaaactta
    18301 ggacaagtag gtgtaggtag taagatatca agtataaata aagatatggt acaaggaaat
    18361 gtacaaccaa ctaatagacc atctgattta atgatagatg gagatggata tcttccagta
    18421 gtaagacaag gaacagttat gtatacgaga gatggttctt ttaatttaga tacaggattt
    18481 aaagcagctg atggtgcttt gaagaatatt aaaggtggaa gacttgtaaa tggtgatgga
    18541 tatatgttac aaggtatgat ttatgatggg gaatataaag ctgatactgg agaatttgtt
    18601 aaaaagggtg atgctaaagg aaaagagcct attgtaatac cacttcaaag tataagtcct
    18661 aaggatggaa cagttcaaaa tgtactagag tataatatat caaaggatgg aacagtagaa
    18721 tttttattga gtgatggtca aagaactcaa tttgtctatg atatacctga tggtgctaag
    18781 gtaggagata cacctgctgg aatagataaa ttaaaaggta caatacaaaa agtgcaaaca
    18841 tatgcattcc aaaatccagc aggactagat gcagaaggag gaaacttgtt taaaccatca
    18901 gcaaactcag gagacccaca aatagcagga gctacaggca caataataca aggggcaata
    18961 gaagcttcca atgtagatat agcagaagaa tttacagaaa tgataatagc aagtaggtct
    19021 ttccaagcaa acagtaaaat aatatcgaca tcagacgaga tacttcaaga aataataaat
    19081 cttaagagat agtaggtaca tattatgata agagtaacgg atttaaacgg aagaaaatac
    19141 atattaaatt cagaccttat agtgaggatt gattctgtac cagaaagtag gattctcctt
    19201 acaacaggag aaaaaaacct tgtaaaagag tcggtagatg agatagtaga taaagtaata
    19261 aagtataaaa gaaaaataaa tgtaggttat attaggagcg aaaaataatg aaaaaaacag
    19321 attcactgac atttattgga ctaatagtat ctacagtatt agttttagtt ggtgccgcaa
    19381 aaggaagtag ttcagggtta aagaattttt ttgatgtttc atcaatatta atcacagtgc
    19441 ttggttcatt tggagctcta atgattacat ttactataga tgatataaaa cttataaaaa
    19501 atgcattaca atactcattt aagacaatga gtgtatcaaa gttagattta ctagaacaat
    19561 ttaaaacact ttctaaaaaa gctagaaaag aaggtctttt atcaatagag agtgatgtaa
    19621 tgggaataga agatccattt atgaagaaag ggttagagct gtgtattgat ggattggaaa
    19681 tcaatgagat aaggtccatc ttagaactag agatagattc tgttgaagat acaaataaca
    19741 gatactctaa actatttaaa acttggggaa cctttgcacc tgcttttggg atgttgggaa
    19801 cactaatagg tttaatacaa atgttagctg accttacatc tccagattta atagcatctg
    19861 gcatgggaaa ggcattaatt acaacgtttt atggttcttt acttgccaat atcgctttga
    19921 acccaattgc atataatata gatgagaaga ctgaaaaaga aatttatgta aaggaaatga
    19981 tgttggaagg tataataagc atacaatctg gggagagcag tatagtagtt gaggaacgtt
    20041 tagccacata cctttcaaac aatgagaagt tagaaattat gaaatccaat aaaaatacag
    20101 aaagagcaat gagcaatggc gcgtaaaaaa aataagaaaa aggatgagat aaatccagat
    20161 gcatggctgg ctacttatgc agatactata acactgattc ttacattttt cgtattattg
    20221 tatgcaacat cagttgtaga tatggaaaaa tttaaaaaca tagcagatgc gttaaaaggt
    20281 cagttttctg ggacaagtat atttagtacg ggttcaagta caagtgagaa atctccaata
    20341 gacagtgttt tagtggataa aaatgataaa tcaaatttag aacaggattt aaaagataaa
    20401 gttaataatt ctaacttaca aaatagtgta acagtcaaag atgatagtag agggatttta
    20461 ctagagttag atgattcaat tttatttgac tctgggattt ctgagttaaa agtagatagt
    20521 aaagctactc ttaataaagt atatgacatg ataaagatga tgaaaaataa gatagttata
    20581 gaaggccata ctgataatgt acctgtaaaa tcaagtacac atgaatccaa ttgggattta
    20641 tcttctagta gagcagtaag tgttgtaaga tactttgtag aagaaaaggg catggaccct
    20701 agactatttt cggcaacagg atgtggagag tatcaacctt tggtttcaaa tgaaacagca
    20761 aagggaaggg ctgagaatag aagagttaat atacttataa ctattgacta ggaggaagag
    20821 gtttgttcaa aaataaaaaa attattataa ttattttgat actgattatt ttctcagcgg
    20881 gagcaggttt ttttatatta ggagaaggtg gaaataaaca agttgacaag attgcagatt
    20941 tgtttattcc aacaggaaaa ggtaaagatg aagtaaagac cgaaaatctt gacttagaag
    21001 agatggtatt aaagctagct gatgaaggtg taagatattt aaaagtatca ataactgtat
    21061 cttatgattc ttcatataaa gatatagaaa aaaatacgcc tttaattagg gataatatta
    21121 taaattgttt tatggcaaaa agggcagatg attttactgc tgataattta aagaacataa
    21181 aagaagaaat aaaaaataca ttaaatatac aactaggtga aaatgtaatt caaaatatat
    21241 atttcacaaa tatagtagtg caataaaagg atggtaagac atgatacttt atttactaaa
    21301 aatattaata tgtatcccaa taattttaat attgatagtc ttatctttaa aaattagcca
    21361 aggaacttta ttaaaaaaat ataatgataa atatgtaaaa atactagata ttgtaaatat
    21421 aagtaaaaac aatagcataa taattttaaa aataggtgaa gaaggatgca ttatggcatc
    21481 ttcttcttct agcatgagta aaataaaaga tttgaccaaa gaagaaatat taaaaataga
    21541 aaacgacatt aaagaaagaa attttaaaat tcaagatttt aaaatggaaa gaattaaaaa
    21601 gaaatatgat ttcagtagat atattggaaa atttacaaaa aaggaagata agtaaatgga
    21661 actattatca aaattacctt tggctgatgt accatataca agttcaatgc aattgttttt
    21721 atttctaact gcattaatgt ttttaccatt tgtaatgttc atgatgacaa gctttgtcag
    21781 aatagttata agtttatcat ttttgaaatc agcattagga gcacagcaag caattccaag
    21841 tcaaattttg gtaggacttg caatagtatt gacaatattt ataatgagac cagtattaaa
    21901 tgagataaac gaaaaggcat tacaaccata tatgaaagaa gaagttacta tggaagaagc
    21961 tatgaaggaa gcagaaggac ctattaaaga gtttttatta acacaaacca gacagacaga
    22021 tttagattta tttgtagaac aggctggttt aaaagaaaag aaattaactc gtgaaaatat
    22081 accactatct gttgtagttc cagcatttgc tataagcgaa ctaaaaactg cttttcaaat
    22141 aggtttttta atatatatac catttttgat aatagacctt gttgtggcaa gtgttttgat
    22201 gtctatgggt atgtttatgt tgcctccagt catgatatca ttaccattta agttattatt
    22261 atttgtaatg gtggatggat ggaacttgat agtaaaaaca ttgatattgg gatttggata
    22321 ggagaaaaat agtatgagtg aatttatgat aatgagtgtt cttaaaaatg gcatgtttac
    22381 aggagctatg attgcagctc cagtactcat tgtttctctt gtagttgggc ttttaataag
    22441 tgtgtttcaa gctacaactc aaatccagga gcaaactttg acttttgtac caaaactaat
    22501 agctattctt ttagtagtag ctattggtgg aagctggatg ttacatactc ttattagttt
    22561 tacttctgag atattcaata tgatatctct tataactaaa taagtaggat acatatgatt
    22621 tttgtattta ttagaatagt agcattcttt ggaacactaa gaatactatt tccagctggt
    22681 acacctgctt tatttaagtc actctttgca atcattatat caatattgat ttcaagtact
    22741 atgcaaattg aatatgcaac aaatatagac aacattgtat tgtttacctt atatggtgta
    22801 aatgagacca taactgggat tatgcttgga tatataacca acctatgttt ttatagtatc
    22861 agaatggcgg gttctatgat ggaccaacag atgggattat caatgattaa tatgtttgac
    22921 ccaaattcaa tgactcagac aactttaatt gataatctta tgaactggac agcacttatg
    22981 atatttttta gcatggatgg tcatcatgtt ttaattagag gtataaggta tagttttgag
    23041 ttaattccaa taggaaaacc atttgtagat aataacatag attatataat aaacatattt
    23101 gtgcagtgct ttttaactgg attcaaaata gcaataccta ttgtactttg tctgcttatg
    23161 gcagatttta ttttagggct tatatcaaga agtgtacctc aattaaatgt aatgatagtt
    23221 ggtatgccat taaaaatatt ggttggtatt gcattattta tcatatcaat acctttaatt
    23281 gtcaatcaaa taagtcattt attaagtcaa ataccaaaga tgtatgaggg aacttttgca
    23341 ctagctccta tgttttttat gggttcagca gataaaacag aagaagctac tccaaagaaa
    23401 aagggagaac aaagaaagaa aggtaatata gcaaagagta gagaattacc cgttgcaatg
    23461 actctacttg cattcacctt attagtacct acattatttt catatgtagt agatacactt
    23521 aaatcttcgc ttaattattt tttgagtcta gatttttata tgaacataaa ttattcaaac
    23581 ctagagaaat tagttatagc aggattaatg gattttttta agatattttt accaatagct
    23641 attccctttt tagttttagg cataatagca aacctatttc aggtgggaat actgtttact
    23701 ggtgaaactt taaaaccaaa tttatcaaaa ttaaatccaa ttagtggatt caaaaacatg
    23761 ttttctatga gaagtttgtc aaccttaata aaggatacag cgattatatc tattttggct
    23821 tacataggat atacattctt ccaagataat tatttagata tattaaagct aggtaacata
    23881 tacctaccaa ctttaatgta cacagttaaa gacttagtat attcaatatt gagtaagatt
    23941 tgtgtagcta tgatagctat agcagttgca gattatgttt atcaaagata cagtcataaa
    24001 aagcaactga gaatgacaaa acaagaagtt aaagatgaat ataaaaactc agaaggagac
    24061 ccagaagtaa aagctaagat aaaacaaaaa caaagacaaa tttcgtctca aaggacaatg
    24121 caagcagtac caagtgctac tgtaatagta accaatccaa cccacttatc tatagcagtg
    24181 agatatgaaa aagggaaaga ccaagctcca gtagtggtgg caaaaggtgc agattatcta
    24241 gcttttaaaa taagggaaat tgcaaaggga aataacatcc ctataataga aaacaaacct
    24301 attgctaggt tgttatataa gcaagtagaa atagaccaag aaattccaga ggacatgtat
    24361 caagcttttg cagagatatt agtggcagta tataaaatca aaaatagata caaagtcccc
    24421 aaaaggtagg ataagtaatg aataagttta atttaaagga aaatatggat gtaatagtgg
    24481 catttggtgt tgtagggatt gttcttatga tgatagtgcc attacctaca tttatattag
    24541 atattttact ggcaattaac ataagtttat ctgtgctaat tttactaatg acattattta
    24601 caacaaatgt acttgattta tctatatttc caacagtgtt acttgtgaca acactattta
    24661 gattgggatt aaatctttca tcaactagat tgattttaac acagggatat gcaggtgaag
    24721 ttatagaggc atttggaagt tttgttgtag gtggaaatta cgtagtagga ataattatat
    24781 tcttaattat attaattgtt caatttgttg ttataaccaa tggttctgga agagtatctg
    24841 aagtaacagc tagatttacg ttagatgcca tgccaggtaa gcagatgagt atagatgcag
    24901 atttaaatgc aggagttata gatgacaaaa ctgctagaaa aagaagaaaa gagcttcaac
    24961 aagaagcaga tttttatggt tctatggatg gagcaaataa gtttgtaaag ggagatgcca
    25021 tagcaggtct tatagtaaca gcaataaaca taataggtgg aattgtaata ggagctgtaa
    25081 tgctgaatat gactcttatg ggtgcagcac agacatatgt aagacttact ataggggatg
    25141 gtcttgtaag tcagatacct gctctattaa tatctacatc agcaggtata atcgtaacaa
    25201 gagcttctac tgatgaaaac tttggtgact tagtaggtaa acagttaacg gcaataccaa
    25261 aagcagtaat gatgactggt tgtgttctaa tcgtacttgg tatgatgcct ggattaccta
    25321 aaccagcatt tttttcacta ggtataggag ctatagcagc aggttacttt ttaggaagag
    25381 aaaagaaaga tactttagaa gacttggagt ttgaaaaacc tgatatggta aacccagata
    25441 aaaatgaagt agaagatgta acaaatttaa taaatgtaga atcaatagaa gtagaaattg
    25501 gatatggttt aatatcctta gctgatgaat cagcaggagg agatttactt cagagaatag
    25561 catcaataag aagacaatgt gcaatagata tgggtatagt cgtacaaccc ataagaataa
    25621 gagataactt acagttaaat ccaaaccaat acaatataaa aataaaagga aatgtagttg
    25681 gtgggtatga gataatgcct aatatggttt tatgtatgga ccctatgaat caaggattta
    25741 gtatatttgg aataaagaca attgaaccta cttttggaat agaggcactt tggatagaaa
    25801 gtgacaaggt agaagatgca gagttaaagg gttatacagt tgtagattca tccacaatac
    25861 ttataactca cttactagat ataataaaat caaatgcaca cgaattactg ggaagacaag
    25921 aagttaagac tattatagat gcagcaagag aaaattatag tgctgtagta gatgaattga
    25981 taccagattt gatgacactt ggtgaaattc aaaaagtact taaaaatctg ttaagagaaa
    26041 aagtaaatat aaaggataga gttacaatac ttgaaacgct tgcagaccag tctagaaata
    26101 caaaagatat agagcttctt actgaatatg taagaatagc cttggctaga agtatttgta
    26161 ataatttagt tgatgatgat aagacaattg tagtggctac attgtctcca gaaactgaag
    26221 agttggtatc taataattta cagaggtctg taaatggaac ttatccagca gttgacccag
    26281 aaaatacaaa taaaatattt gaaagtatac aagaagtaat gagcaatgta tatttcaatc
    26341 ataatatacc agtaatagta gtatcaccaa agataagagc tccatttaga aaattagttg
    26401 aaattgtata tcctaatttg actgtattat cattaaatga aatacctaac gatattaaaa
    26461 tcaaagcaga aggtgtggtg agcatatatg atgacgaaaa agtatacagc taacacaatt
    26521 caggatgcta tgaatctagc caaaatggaa ttgggtgaca atattacatt aatagataaa
    26581 aaagaggtaa gaaagtctgg aatacaaggt atcttttcaa aaaaagatat tgaactaact
    26641 attggatggg aaaagagaga aaatgtagaa caaaaagatt taaaacgaga gatagaacaa
    26701 ttaaagtcaa ttattaacaa tatggggttt gataataaga atgataatga tatagataaa
    26761 atatgtaaaa atctactaag tttagaattg aatgaagaaa ttgtagaatc tataagaatt
    26821 gatttgcaag aaatgaagtt caatggaatc gatacaagta aaaatttagt agaaatttta
    26881 aagaaaaaga ttaaaataga aaatcaagta ataaatggta aaattgctct agtcggacca
    26941 ccaggagtag gtaaaacgac tacaatagca aagttagccg caaaattagt ttttgaagaa
    27001 aataaaaaag taggagtaat aactatagat acatacagga ttggtgctgt agaacagttg
    27061 aagatatata ctgatataat gaatattccc tttaaagggg taataagtcc tgatgaaatg
    27121 gaattggcac ttgacgagat gaaagattgt gatgttgtat taatagatac aacaggtaga
    27181 ggctacaaaa actctatgca aatactagaa atcaaaaatc ttatagataa agctgaaact
    27241 gataatatac atttagtttt gaattgtaca acaagagaaa gtgatacaaa agcaattata
    27301 gattcatata gaaatgtgaa ttttaaaagc ttaataatta caaagctaga tgaaacaata
    27361 acatatggct caatatttaa cataatgaat tatgcgcaaa aaccaatatc atatattaca
    27421 acaggacaaa atgttcctga tgatattata aaacctaatg aagataagat tataaggtta
    27481 ctattaggag ttgaaagtat atgatagacc aagctcaaat tttgagaaaa atagcaatgg
    27541 aaaaaagagg acttgatgag tttatagtag agaatgagaa tgtacctaag ataattacaa
    27601 tagcttctgg taaaggcggt gttggaaaga gcaatctcgc tacaaatttg tctatatgct
    27661 tgaccaaact taataaaaaa gttcttatat tagatgcaga cataggaatg tctaatatag
    27721 atataataat gggggtaaat gtaaaaggga ctataattga tgtaattaat ggcgaaaaaa
    27781 atatagaaga tataatttca cagactaagt atggtgtaaa tataatatct ggaggttctg
    27841 cccttaatca tatagaagac tttacagaag ctcaaagaaa taagtttata catagcatag
    27901 aacaaattca cgatgtggat tttataataa ttgatacagg agctggtatg agcaaaagcc
    27961 tattgtcatt tatatactgt agtactgaat ttttcttaat aacaactcca gaaccgacat
    28021 cattgactga cgcatatagt ttattaaaag ctataagtaa ttttggcata aagagaagtg
    28081 caaatataat aatcaatagg gtaattgatt tagaagaagc aaaatctaca tacaaaagaa
    28141 taaaaatggt tgtggacaaa tttctaaacc ttaatctaga attgtatgga tatataatag
    28201 atgacaaaaa agtaagttta tgtgtaaaaa aacagatacc attcatttta gaatatccta
    28261 caagtattgc atcaaaatgt attatagcca tagcaaagag aatggtaagt cagtcaagag
    28321 aagatattgt tgataataaa agttttctaa gaaagatgtt tagtatattt aaaagctaag
    28381 gaggcgtagt taatgaatag agaagaatta ataaaagaga atatgcctct tgtaaagagt
    28441 atagcaagaa aattttttat accagggaaa ggttttgaat atcaagactt agtaaatagt
    28501 ggagtcattg gattgattga tgcaataaat aaatttgatg ctgaaaaagg ggcaaagttt
    28561 tctagttact cttatataaa aataaaatct gctatattag atgaaattag aaatcaaagt
    28621 ccaatttcta aacataatct aactaaagta aataagtata atagagtggt agaaaagttg
    28681 caatctaaat tattaagaga gccaacttca tatgaaattg caaaagaatt aaaggtttca
    28741 gaaaaagaac ttcatgatat cgaaagtaat atagatatgt taaatattgt atcattaaac
    28801 tatgtaatct ttgaagatac aaatgaaact gtacaagatg taataagtga tagagaagag
    28861 gaagctccag aaaatataat agaagaagaa gaaaagttag agattctatc caaagcaatt
    28921 agtaatttaa atgaaagaga aaaattaata ctttcactgt actattatga agatttaaat
    28981 ctaaaggaga tagggaaagt gctaggtgtt tcagaatcaa gagtttcaca actccacaga
    29041 aaatcaataa gaaacttaag aaacaagata aaagaactta aatattctat atagatggtg
    29101 attgataaga tgatacaaat tatattaata gtagtgtcta ttttaataat agtaactata
    29161 gttataaatt tacataagga atcaaaaaat aaagataaca gatactttga taaaatattt
    29221 gagcaagaat atgaagcaaa tgatagatta ataattgcag aaaaaagaag attcttttta
    29281 gacaaagtac ttgcagaaaa aagaattaat agtcatataa aagtttatga aaattataac
    29341 ctaataaagg ctaatgaaga aagaaaggaa aacatattta tagaatcaaa aaaggaagta
    29401 agacaagaaa atgagttatc tcttaaagtg aaacatctta taagttcagg ttatgatgat
    29461 gtggaaatat gtaagatgct agatattgga aagggggaac tgttattaat aaggagttgt
    29521 tacaaaatat aaggatatta ttatgtgata aaaaagtatt cttaggaata ggtattggta
    29581 tattaatatc aactttatgt ataatgcttt ttagtaataa tgatatgagt aaggcagaga
    29641 tagaaaaaaa ggccagagcg ttaggcatga aatatacaag tgagatgaaa gttttagaat
    29701 agatgatatt gtttagacat ttacaaaaaa ggaggtcata atgtgataag aggattatat
    29761 acagcagttt ctgctatgat aactaatcaa caaaagcaaa atgttgttgt caataaccta
    29821 gctaatatgg atacaaatgg atataaaagt aaacaacttt tgactaaaag ttttgatgaa
    29881 ttaaagttgg agggttacaa taactacgca aatggaacta aacaaaagca gattataggc
    29941 gatataagtc caggtatttc tatggatgaa actatcacaa attttaatca aggacctata
    30001 aaaactactg ataataaaat ggatgtagct attcaaggta agggattttt tctagttagt
    30061 gatgcagctg gaaatcaatt ttacactaga aatgggaact tcagaacaga caatcaaggt
    30121 gaccttataa caagtgaagg gtattatgta cttgggacga atactgctac tggtgctgta
    30181 ggtcatataa atgtaggaaa tcaaaaattt gaagtggcaa aagataatac aatatcacta
    30241 gacggaaatg ctatgtataa atttaatatt gtagatttta ataactacaa taacttaaaa
    30301 aaagaaggag ataacttata ttctggagga ggagctatga aagtaaataa tgcacttaca
    30361 atacaaagtg cgatagagtc ctctaatgta gatatggtat ctgaaattaa taatatgatg
    30421 acaatatcaa gagaatatga ggcaaatcaa aagataattc aggctatgga ttctaaatta
    30481 gcaaaaatag caagtgagat tggttcagta agataggtaa taggaggtga atttttatgt
    30541 acacaatgtt ttatactagt aaaacagctt tatcagcaaa tcaaagtaaa ctagatataa
    30601 tatctaacaa tatagcaaat tctgattcaa caggatataa aaaaataaat atggaatatt
    30661 ctgatttagt ggatgaagta ctaaatagac catcatatcc tacaaatggc aatgatatat
    30721 cgactggaac aggagctaag gcaacagctc ccatgagaaa ttatgaacaa ggtgcattgg
    30781 ctcctacaga tagtaagagt aatttggcaa tagatggaga aggattcttt agagtgataa
    30841 gaaaagatgg tacatatgca tatacaagaa atggtggatt taatgtagat gctttaggta
    30901 agattgtaga tgataatgga aatatattag atgtccaatt tgatgcagga tataactata
    30961 ataatacaaa tatagatagt acaaacctga cagtttcaag aaatggagag ctattcgtag
    31021 ataataaaaa aataggaaaa atcaacttat atcaacctat aggaactcaa aattttatat
    31081 ctgaaggaga tagtttattc gtcgcaactg atgcagcaca gattaaacaa gttgagaaag
    31141 tcgatatagt tcaaggctat agagagaggt caaatgtttc tttacaagaa gaatttgttg
    31201 acttaatagc cactcaaaga gcatttcaaa tgaactcaaa gggaataaag acagcagatg
    31261 aaatgtggca aatagcgaat aatttaagag ctaaataatt atgtaaggtg aggaatattc
    31321 atgaatgata tgaaagagta cgattttggc aaacctcaga gttattcaag agaaaattta
    31381 acttgtttaa attttttatt agctgaattt tgtaaaaaat ataaaaacta tataatttat
    31441 gaacttaagt gtaattctag tatggttgtt gatagtatag accaagtaaa ttatcagact
    31501 tttttagata gagtaacttc ttcatgcgta gtagtacaaa atgcaatgga acctgttatg
    31561 aaaaactttt cttttagaat agatagaagt gtagctgata tgtggattga tataacttct
    31621 ggaggaactg gagttgtaaa ggataatgat agagaactta cagaacttga taaaaaagtg
    31681 cttttacacc ttatggatga tttagtaaga aatatgcact tatttgaggg ttttgaaaat
    31741 atacaagtat tggatacaca tacacatatt aatttgccac aattatgttc tccaactgca
    31801 ccagtatgcg ttgtagattt aaaagtgcta aatgataatg agtatattgg aagtgtttct
    31861 ctttgctttc catacaatgg tttagaggct ctgttagaaa gtatatctgt tatagaattt
    31921 tttgataatg atgtaggaga tgattcagag gaatttaccc aaaaaatcta taatagtgta
    31981 tctaatatag aattatcagt tattgcagag ataggaaaag tatcaattaa tgttgaggat
    32041 ttactcaaac tagaagttgg agatgtaata gtaacaaata aaaaaataaa tgattatata
    32101 gatatatttg tagaaaattc aaaatcatac acagcaacac caggatttat atcaagtaaa
    32161 aaaggtgtaa aaataaatga tgctgttgga aaagaggtgt agttatggac aatagtaatc
    32221 ttatttcaga tgaagaagca aagatgttat taaatgataa agttgaaagt gaagttgatg
    32281 gagatgatga gttagcctta gatgtctctg aagttaatcg aaataatata caaaggatac
    32341 ttgaccttaa gttagaatta tctgtagtta taggaagaac taagatgact ctaagagata
    32401 ttttaaattt acataaaggt tctcttattg aactggatac tttggcagac caagatgtgg
    32461 aaatactaat agataataag gttttggctt atggaaaagt agtggtggta gatttaaatt
    32521 ttggtgtaaa gataacaagt atagtaagtg aagaagacat ggtaaaatct cttgtttaga
    32581 gataacatca attaatactt aaggcatgag gtggtactta ttttaaactt aaatgatata
    32641 aaaaaagata tactaaaact caataaaaaa gactcatgtt ctctatataa tgaatctatt
    32701 aataatataa ataataattg tatatcaact ggattaaaat ttttaaaaca agcaagagat
    32761 ttaaacccag atgatgttga tatattaaat ctcacaggtc ttgtaaactt actaaaatgt
    32821 aattttgatg atgctgtaga aagtttctat aagagttcac tttgtgaaaa aactgctctg
    32881 tgtgaaaagt atataaacat gcttacatct gaggagtttt taattttctt tgaaaagtac
    32941 aatcaactta taaggttaat aaataaagga atgtataaag aagctataca agggtttaaa
    33001 cttataactg accaatactg tgatttaata gaaccttatg agttgcttgc cttactgtat
    33061 gacaaggaag aagaatatat aaaatttgat gaatgtttag aagtattgaa gattattgat
    33121 aaggaaaatt atttattaaa tcaagaaaat aacacattat atatgtataa tagtgctaat
    33181 atacaaacta aaagtaatat aaaagtagat gattataaga atgagttctt acataaaagt
    33241 agtagtaacc ataaaattaa tgattataat gatgtaacta aagaaaagag ttcaagcaaa
    33301 aaccagaaaa gttcagattc taataagaaa aaaagtatta aaaaaaataa gtacctatat
    33361 atagccataa tattaggaat attattagta ggacaaagta tatatagttc ttataagatg
    33421 aataatttaa ctgataaaat atctaaaaat aatgaaacta aaaatgaaga gtcaatagat
    33481 tctaacttga ctaagaaaga aaataatata gaagaaaatg aagatatgag taaggcttct
    33541 aatataaata agagtaataa aaacattaat aaagaggatg atgtaaaatc taaaaaaaat
    33601 actgtagaaa atgaagatga taaagattct gacttattta cagaggatga attgatgtct
    33661 aaggcgaata ctttaaaatc agaaaagaaa actaaatctt ctatacaact atataagaaa
    33721 gttgcagata taggtgaaaa taaagggaat acatctgaag caacttatca agttgctatt
    33781 ctcagtgaaa agttaaaaga ctataaaact gctgaagaat attataaaat gtatgtagaa
    33841 aattactcag aaaaagatgc ttattttgat gaatcttatt ataatttagg tatgatgtac
    33901 tataacaatg gtgatttaaa aaattctaaa ttgacattaa agaagttggt taataaggta
    33961 ccaaatagta tgtataataa ttcaaaagta aaagaaattt taaaagaaga atagttaaat
    34021 tagataagaa aaataaatta ttttatttta aaataatatg attataatat attgacatac
    34081 cattttaaaa aatttaaaat attaattata aaatgaggag ggataaaaaa tggaatttga
    34141 aaaagggagt atttcaattc atacagaaaa tatttttcct ataattaaaa aatggttata
    34201 ctctgataaa gacatattta taagagagct tataagtaat ggatgtgatg cagtaagtaa
    34261 acataaaaga cttgtatcac taggagaaat atcagaaaat aagtcatcag attataaaat
    34321 tacagtatct gtaaataaag gagaaggtac acttaaattt attgacaatg gaattggtat
    34381 gactgaagaa gagataaaaa aatacataaa tcaagttgct ttttcaggtg ctgaagattt
    34441 ctttaataaa tataaggata aaatggaaga gtcaaatgat ataataggtc attttggatt
    34501 aggattttat tctgcattta tggtgtctaa aaaggtacaa atagatacac tttcatatac
    34561 agaaggagcg actcctgtaa gatggatatc tgaaggtggt acagaatacg aaatatctga
    34621 atcagatgct agaaatgata gaggaactac tataacacta tttattgatg atgatagtaa
    34681 agaattttta gatgaattta ctgtaagagg aataataaat aaatactgtt ctttcttacc
    34741 agtagaaata tatttagaag atgttgaaag attagaaaga gaagctaaag aagcagaaga
    34801 aaaagctaaa aaacaaaagg aagatggaaa agaagagatt gtagatgcaa aagtaattga
    34861 gcctttaaac gacactaatc ctctatggtt aaaatctcca aaagactgta ctgatgagga
    34921 gtataaagag ttttatagaa aggtattcaa tgtatttgat gaacctttat tttggataca
    34981 tttaaatgta gattatcctt tcaatttaaa gggtatactt tacttcccta aattaaaaaa
    35041 tgagtttgaa ctgactgaag gaaaagtaaa attatacaac aatcaagtgt ttgtggctga
    35101 caatataaaa gaagttatac cagaattttt acttttatta aaaggagtta tagactgccc
    35161 agacttacca ttaaatgtat caagaagttt cttacaaaat gacagggatg taagcaaaat
    35221 ttctaaacat ataattaaaa aagtagctga taagttgaaa tctctttgta aaaatgaaag
    35281 agaagaatac aataagtttt gggatgatat acaaatattt attaaatatg gttgcttaaa
    35341 agatgaaagt ttctatgaga aggtaaaaga atgtatatta ttcaaaacta ttgatgatga
    35401 gtatataaca ttacaagatt atttagaaaa atgtaaggac aaacatgaaa ataaggtatt
    35461 ttatgtaagt gataaggaac agcaatctca atacattaaa ctatttaaag aatacgactt
    35521 aagtgctgtt gttttaaact catcaataga tactcatttt atttcattta tggaatataa
    35581 agagaatgga gttaagttta atagaataga tgcagacctt tctgatgtat taaaagataa
    35641 aaatgaaaat aaagatagtg aagaaaacaa agaagagata gctaagatag aaggtctgtt
    35701 taaagaagct gtcggagaaa gagtgaagaa ttattctgta gaaggattga aaaatgaaga
    35761 cactccagct atggtattag tatcagaaca atctattaga atggcagaaa tgcagtcaag
    35821 atttgctgga atggatttag gaatgaattt tgaggaagaa aaaactcttg taataaatga
    35881 gaacagtcca ataatcaaaa aattggtttc tcttaaagat gatgaggaaa agaaagataa
    35941 gattacgctt atttgtaacc agatagcaga tttagcttta ctttctaata aagaattaaa
    36001 gccagatgag ttagattcat ttgtacaaag aagtaataag cttatgagtc ttttgataga
    36061 attataagat tgaatttgaa tacattgaag caaatttata aaaagtattc aatatcagtg
    36121 ataagaaaga agtgtctcaa aatgaacttt tcagttcatg aggcgctttt tttatgtgaa
    36181 attaaattta ttttaatcat ttcaacaatg aaaaatatat ttatctctat tttgagacag
    36241 accattcttt atttttttat ctttggttaa ttttaaatat ttaagtaggt tctaataaaa
    36301 aactctattt agttaaatat agttaacttg atatataata agataataca tataattaag
    36361 taaaagattg ttttttagtt tatgtttaaa tacataatta cactttgtta aaaacataaa
    36421 attattacta aagtagaaat aggaggtttt agaatgaatt ttaattataa tttgccagta
    36481 aatatcttat ttgggagagg cagaattaat gatgtgggaa aagaagtatc taaatatggc
    36541 aaaaaagtat tgatagtaac aggtaaaaat agtacaaaga aaactggatt acttgataaa
    36601 acaatagatt tactaaaaga ctctaaaata gaatatgaag tttttgatag agtagaacaa
    36661 aatcctctta taactactat atattcagga gtagaaatta taaaatcaac tggatgtgat
    36721 tgtgttttag gtcttggtgg tggaagcatt atggatgctg ccaagagtat agctttttct
    36781 tataaaaacc caggagattt aaatgattat atatttggaa ttagacaagg tgaagaagct
    36841 ttacctataa tcttagttcc aacaacatca ggtacaggta gtgaaggaaa ttgttttgca
    36901 gtacttacaa attcagaaac taaagataaa aaatctataa agaagaatag tatgtatgca
    36961 aaagcatcaa taattgaccc agaacttatg gttacaatgc caaaacatat aattgcatca
    37021 gttggatttg atgcacttgc ccataatatg gaagcatatc tttctaatgg aagaaaccct
    37081 cttgcagatg tacaagctat ttatgggatt gaacttatat cagaaaacct aataaaggta
    37141 tataatgatg ttaatgattt agaagcatgg gaaaaaataa cacttgcaag tactattgga
    37201 ggaatgacga ttggtacagc gggtacagcc ttgccacatg gaatggagca tcctgtaagt
    37261 ggtcttagag atgtaatgca tggaaaagga cttgcagctt tgactcctac tattatagaa
    37321 agttcatggg atagtgatat tgaaagattt ggtaatattt caaaggtatt aggaggaact
    37381 tgtgcagaag attgttcaga tgcaattaga aatttcctta aaaaaataga tttagatata
    37441 aaactaagtg atttaggtat tgaaaaatca gatatagatt ggttaactga taattgtatg
    37501 aaaatatcag ttgcaaatat taagagacat ccaaagtatt ttaataagga acaaataaag
    37561 gaaatgtacc ataagtcttt ataatttgac tttacatatt tagttttata taaaaattat
    37621 aaattaataa taaaaaatta taagtaatta atagtaaaaa aattgtgtat agaagttata
    37681 tatataataa atttgtctta agcttttatt ttactttcta ataaataaag gagtttaaaa
    37741 aagggaagat taattaagta atattttata aaattcttcg atttaaaaac tttaagtaga
    37801 ggtgaaataa atgtttaatc ccaaaagaat aatattcgaa aaaggaactt tagacactga
    37861 tgtgggtaaa aaaatataca ataaatttag agataataaa gatgtagaga taattaatat
    37921 atcatcaaat aggattaaag aacatattcc aggtgaaaat ctatatgaac aatatagaag
    37981 tgggaagcag actatggtag ttgggattag aaaaagtctg aagtttcaaa catgtaaacc
    38041 ttctgctaat tatcaactac cacttgtcag tggatgtatg ggaagatgtg agtattgtta
    38101 tttgaacaca caattaggtg ataaaccatt tataagggtg tttgtaaatg tagatgaaat
    38161 acttgaacag acaaaaaaat atattgaaga tagaaagcca caggtaacca tatttgaagg
    38221 ggcagctaca tcagacccag ttccagtaga gccatatact aattctttaa agaaaacaat
    38281 agagtttttt ggaagagagg aaaaaggaag atttagattt gttactaaat acagtgatgt
    38341 ggatagctta ttggatgctg aacataataa tcatacagaa atacgattta gtatcaatac
    38401 tcctagaatt ataaatgaac atgagcatta tacagcctct gcaaataaaa gaatagagtc
    38461 agctgtaaaa cttgctaggg ctggttatca gattggtttc ataatagcac ctgtgttttt
    38521 atatgaaggt tggaaagaag agtataaaaa tctgattata agtataaaag aagctttacc
    38581 aaaggatttt gataaaaaaa ttatatttga agttatatct catagatata caactaaagc
    38641 aaaaaataga atactagaga tttatccaga tacactattg ccaatggaaa atgaggatag
    38701 aaagtttaaa tacggtcaat ttggatatgg taagtacgtg tatgataaag aacaattaga
    38761 tgatatgaaa gagttctttc ttaaggaaat aaatggagta tttaaaaata gtttaataaa
    38821 atatataata tagcaaatct tacctaaaga tttgctatat tttattttta tacattttat
    38881 gtttataata aagcaatgag atttagcact atcatataag aaaattaaaa agggcactat
    38941 ctatcaagaa cattttttct agttttgata ctataaagga taagagtaac aaaaatatca
    39001 ggaggagtta tagtatgcat gcatgggagt ctattcaaat tacattggat ttaatagaag
    39061 caaatttatc agaagaaata tcaattaatg aattagcaaa taaagcaaat ttatcaccat
    39121 tttactatca aagactattt aaaagattgg taaataagac tgtaatggag tatataaagc
    39181 ttcgtcgtct tgcaagagct tctgaatact tagcagaaca taagaatcgt atacttgatg
    39241 tagctttaaa ttttggattt gctagtcatg agacatttac acgttccttt aaaaaagcat
    39301 atggattaac tccagaaaaa tatagagcaa atccagtaaa actcaatcac tttattaagc
    39361 cagaattaat tttaaattat gctatggttg atgaaggtgt tccactgatt gcagatgata
    39421 tagtgattga agtcaatcgt aagacattga gtaatccaag gacatttgtt ggaatagaaa
    39481 tagaagtacc tatttgccag ttggtgggcg gtgaaacaac tggaattgca atagtagagg
    39541 aattatggat gaaacttgga agtcaaagac ataatatacc aaatcaaatt ccaggtggaa
    39601 atgaatttgc tgctctttat atgggaaatg caaaagaagg aaattgtatg tatatggcag
    39661 gtgtagaggt agagtcaggt acatcagtag aaggatatag tacttttgag ttgccagcta
    39721 aagaatattt agtatgtgga tttgaggcag aaaactttaa tgagttagtt aattcagctg
    39781 tttttaaagc agataaattt atggaaagat ggatgaagaa acataattta acaactactg
    39841 attttgcaat tgagatgtat taccccacta cacctgaagc agcatatctg gaacattgga
    39901 ttgttccggt tcctattgaa caatagaaag gatgatgaat atgaattggc aagatgcgtt
    39961 ttttcaagat actaaaccta catttgagca aattaatgaa tttattaata atccattatg
    40021 ggacaagtta aataaaactt tggtttctac ttataatgta gaacctaagc ttgaatacag
    40081 taaatgttca atgcaaagag gttggaatgt aaaatataaa aaaagaggta aatcactatg
    40141 tactttatat ccacaagaag gttattttaa agctttggtt attgtaagtg aaagcaatag
    40201 agtagaggtt gatttattta ttaatacttg ttgtgattat atcaaaaaaa tatataatga
    40261 ggtaaacttt tttaatggaa gtaaatggct aatgattcaa gtagatagtt tattggtatt
    40321 aaacgatatg cttgaactaa ttaaatttag agcttaacat atagaacagg atgaatatat
    40381 aagtcatcct gtttttatat tcaatcagat aaattaatat atagtctata atatgaataa
    40441 tttagtaaat ttaaatttac tttatctttc tacattcttt gcaactaagt ccacatgtgt
    40501 ttttttcttg catattagta gcaccccaat tagcaaattg ttgtaaaata ggcacaatac
    40561 ttttaccttt acttgttaat gtatattcaa cttttggagg aatttcatta tattgtttac
    40621 gatgaacaat accagtagac tctaaatctt ttaatgattg agctagcata gtatttgtaa
    40681 tgccatccaa ttttcttttt atgctactat atctaattgg attttcaact gctaacatac
    40741 atataatagg taatttccac ttaccaccga caatgtctaa tgcataacgg attggacagg
    40801 ttaattcttc aacgttttta gttatatttt tttcagtatt tatactcatt ttaattactc
    40861 cttagttata gtagtatctt ttttatactt tatcagaaaa aactgcatac ttgttttttc
    40921 tgtattttat attaaaatta tatcaaagga taatccttaa tacaagtatt taaaaggggt
    40981 gtaagaatta tgaaagcatt agaatttttg aacaaggcac gagtttatta tttagcaaca
    41041 gttgatgaga acaatcaagc acatgtgcgt cctttaggat ttgttatgga atacaatggt
    41101 aaacttacat tttgcacatc taatcaaaag gatatgttta agcaacttat ttttaatcca
    41161 aaggttgaac tttgttgtat agaccaaaac cttaatactc tacgtatttt aggtgaagca
    41221 gtatttgtta catcaaaaga aacacaagct aaggcacttg aaataatgcc aagtttaggt
    41281 cagatgtact ctgtaggaga tggtaagttt gaaatattct ctattgataa ggcaaaagtt
    41341 agttgttgtt caatgagtgg taaaaagatt gatgtagagc tttaattata attgaatcga
    41401 catccatagt gaaaattaag aaaacgttat tactgaaatg ttgatagatt taatttaaat
    41461 gcaataaaaa gatttttaaa taatgaatat gtaaaaattt ttagttatta gatgtgtatt
    41521 tcaataatga tatactattt tgaataatta catactagaa ataagataat taaaatttac
    41581 tttataatgg gataga
//
LOCUS       DACULQ010000012        41410 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.012, whole genome shotgun sequence.
ACCESSION   DACULQ010000012 DACULQ010000000
VERSION     DACULQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.012"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..2608
                     /locus_tag="JA154_02805"
     CDS             <1..2608
                     /locus_tag="JA154_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT7994787.1"
                     /translation="KIIINEKEKFKVLTDNILGGVKTCLKDEYMTMTYVSQGFLELTG
                     YSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQLEKQDKIEVEYRIVRKDGSIIWILE
                     RSKSINDNKGSECIQGVLTDITEFKKIQEELDSKRFEIQAINDSIVGGIVITEINDDF
                     DSIYLNEGYLNMIGYTRKQLKDELNNKLIKLIFKDDRTELINSINKQLKLSDTFASEQ
                     RVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVDITSAKLLEQELKKSEERHEIILKQ
                     TQNIIIEWDIEKDKMNISPNWYNKFKYDPITENATKNIIKSVDIYHDDMDKFISLIKL
                     TSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDNNGKPYKVVGSLTDIDESKREAEKL
                     KERAEKDPLTDLYNKKASQSLIEEYITLDKKRKGALMIIDIDDFKGINDNLGHLYGDA
                     VLSEVANDLINLFRATDIVGRIGGDEFIVFIKDISKTRDIINKAEELVKTFERSFLGK
                     GRNYKISLSVGIAKFPQDGMDYMTLFRNADIALYSAKGKGKNCYTLYDKKIDKVQYIT
                     NTRNAGDSDNVEMSFKEHITNYIFDILYESSDIEVAVNLILSIVGKHFNVSRMYIFEN
                     SDDNRVCKNTFEWCNEGITSEINNLKNICYKDLGNYLLNFNADGLFYCNDIENLGSDV
                     KKVLKPQGIRSILQCKILEGNEFRGFIGFDECSYNRMWTNDEVNTLTFISKTLNMFLL
                     KMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTYELLFINKKMLESAPNTHVGDLCYK
                     AIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKFEVQANITTTTLKWFDGKDACLIFG
                     SEITSIEAEENT"
     gene            2793..4727
                     /locus_tag="JA154_02810"
     CDS             2793..4727
                     /locus_tag="JA154_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAT7994788.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            4833..5828
                     /locus_tag="JA154_02815"
     CDS             4833..5828
                     /locus_tag="JA154_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7994789.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            5800..6585
                     /locus_tag="JA154_02820"
     CDS             5800..6585
                     /locus_tag="JA154_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7994790.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            6573..7271
                     /locus_tag="JA154_02825"
     CDS             6573..7271
                     /locus_tag="JA154_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7994791.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            7394..7867
                     /locus_tag="JA154_02830"
     CDS             7394..7867
                     /locus_tag="JA154_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAT7994792.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            8064..8903
                     /locus_tag="JA154_02835"
     CDS             8064..8903
                     /locus_tag="JA154_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAT7994793.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            8916..9464
                     /locus_tag="JA154_02840"
     CDS             8916..9464
                     /locus_tag="JA154_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HAT7994794.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            9527..10711
                     /locus_tag="JA154_02845"
     CDS             9527..10711
                     /locus_tag="JA154_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAT7994795.1"
                     /translation="MMSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVA
                     EPCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFA
                     DVDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFH
                     DDQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILC
                     DRSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVE
                     SMSEKPIILAMANPAPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDV
                     KAKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVN
                     RI"
     gene            10876..11265
                     /locus_tag="JA154_02850"
     CDS             10876..11265
                     /locus_tag="JA154_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7994796.1"
                     /translation="MMEYKVNDQELNASMFIPFVNRIWPGDYDTDKTQTALSKTLNIT
                     AYDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQ
                     PGVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            11415..11678
                     /locus_tag="JA154_02855"
     CDS             11415..11678
                     /locus_tag="JA154_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7994797.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            12204..12887
                     /locus_tag="JA154_02860"
     CDS             12204..12887
                     /locus_tag="JA154_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7994798.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGKFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            12898..14139
                     /locus_tag="JA154_02865"
     CDS             12898..14139
                     /locus_tag="JA154_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAT7994799.1"
                     /translation="MKIFSNKDIKIFFLMLISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIILNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISVNGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSIFTISFLKLTNM"
     gene            14211..14894
                     /locus_tag="JA154_02870"
     CDS             14211..14894
                     /locus_tag="JA154_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7994800.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            14891..17221
                     /locus_tag="JA154_02875"
     CDS             14891..17221
                     /locus_tag="JA154_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7994801.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDNGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANIGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNGNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAVSGCLLGSILGLILHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPIKKICELDIIDSINAQ"
     gene            complement(17402..18244)
                     /locus_tag="JA154_02880"
     CDS             complement(17402..18244)
                     /locus_tag="JA154_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT7994802.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            18539..19273
                     /locus_tag="JA154_02885"
     CDS             18539..19273
                     /locus_tag="JA154_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7994803.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGRPIEYTISIFRTDKYQYEIVLSE"
     gene            19283..20416
                     /locus_tag="JA154_02890"
     CDS             19283..20416
                     /locus_tag="JA154_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAT7994804.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLNNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            20515..21264
                     /locus_tag="JA154_02895"
     CDS             20515..21264
                     /locus_tag="JA154_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAT7994805.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            complement(21373..21792)
                     /locus_tag="JA154_02900"
     CDS             complement(21373..21792)
                     /locus_tag="JA154_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994806.1"
                     /translation="MKKALRYLLFLIILLLMYFLAVKLNYDFYTQFYTGYMQDFKRYI
                     FINLISSGGIGLLLGTELLIREYKKDGSWYIDIPRLLLLCFPSFLLSLMPVFFFMFPV
                     GNIPIIGNFIMLDRIPLNIIIFNILFGYFLITSFRKK"
     gene            22348..23052
                     /locus_tag="JA154_02905"
     CDS             22348..23052
                     /locus_tag="JA154_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7994807.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAISRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            23150..24541
                     /locus_tag="JA154_02910"
     CDS             23150..24541
                     /locus_tag="JA154_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7994808.1"
                     /translation="MLSILCLIIIVFMIILVSFNVFLDTYIKTTANEELTKSTEIVEH
                     MDSNFRPIKPPQDDGKLPPKVLSNFMRNVQDKVRMAETQSDADAMVVDSKYNLIFPTR
                     EDDFLKNVDEMELIRECIQNEKLDLNSDANTRISTRNKDYYISTVKITSIYGEQDKYL
                     ILFIDISKTLNLAQKINIVLISVMCFAGILAIFTAVILSEKIAKPIKELCEFAKKMGQ
                     GDFKRTYFDFSDKELVELSTVMNKSAEYLDKYDNEQKIFFQNASHELRTPLMSIKGYA
                     EAIKYNVIDSKHASDIILEESDRLSDMVEDLLYISKIDNITKDFELVECDLREVLSNC
                     GARQNVRAINKGIKFIYEFDEEMVLFECDEKNISKAFMNLIENALRYAKSEIKIVCKY
                     NQKNIVVIIEDDGIGIKKEDLPHVFERFYKGVGGNHGIGLSIVKSIVNKHGGRIYVEN
                     GKKGAKFTILFKL"
     gene            24746..26359
                     /locus_tag="JA154_02915"
     CDS             24746..26359
                     /locus_tag="JA154_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT7994809.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(26516..26686)
                     /locus_tag="JA154_02920"
                     /pseudo
     CDS             complement(26516..26686)
                     /locus_tag="JA154_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432172.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(26722..27552)
                     /locus_tag="JA154_02925"
     CDS             complement(26722..27552)
                     /locus_tag="JA154_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7994810.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            27625..29352
                     /locus_tag="JA154_02930"
     CDS             27625..29352
                     /locus_tag="JA154_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7994811.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSHFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            29389..31251
                     /locus_tag="JA154_02935"
     CDS             29389..31251
                     /locus_tag="JA154_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7994812.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            31584..32273
                     /locus_tag="JA154_02940"
     CDS             31584..32273
                     /locus_tag="JA154_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAT7994813.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYQIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            32378..33064
                     /locus_tag="JA154_02945"
     CDS             32378..33064
                     /locus_tag="JA154_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HAT7994814.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            33081..34988
                     /locus_tag="JA154_02950"
     CDS             33081..34988
                     /locus_tag="JA154_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HAT7994815.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPIEVGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            35439..36128
                     /locus_tag="JA154_02955"
     CDS             35439..36128
                     /locus_tag="JA154_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994816.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADN
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            36564..37103
                     /locus_tag="JA154_02960"
     CDS             36564..37103
                     /locus_tag="JA154_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAT7994817.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            37122..38165
                     /locus_tag="JA154_02965"
     CDS             37122..38165
                     /locus_tag="JA154_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HAT7994818.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            38167..39003
                     /locus_tag="JA154_02970"
     CDS             38167..39003
                     /locus_tag="JA154_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7994819.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            39054..39782
                     /locus_tag="JA154_02975"
     CDS             39054..39782
                     /locus_tag="JA154_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7994820.1"
                     /translation="MYAPIFALVLFSFNDSKSMARWNGFTWKWYGQLLQNESIMSALY
                     YTIVIAILASVISTIVGTISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLF
                     VFVKMEFGFTTMLLAHIMFCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVI
                     LPQIKPGIVSGFLIAFTMSIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALST
                     IMFAVVLGLLLLANKKESIVRGIK"
     gene            39782..40834
                     /locus_tag="JA154_02980"
     CDS             39782..40834
                     /locus_tag="JA154_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT7994821.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            41064..>41410
                     /locus_tag="JA154_02985"
     CDS             41064..>41410
                     /locus_tag="JA154_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT7994822.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQ"
BASE COUNT        16291 a         4318 c         7266 g        13535 t
ORIGIN      
        1 caaaattatt ataaatgaaa aagaaaaatt taaagtactc actgataata tactaggagg
       61 ggttaaaacc tgtttaaaag atgaatatat gactatgaca tatgtaagtc aggggttttt
      121 agagcttaca ggttactctt gtgaagattt aaataaaata tttaaaaata gatttaaaga
      181 catgatttat aaagatgatt tagacaagtt taattgtatt attctaaacc aattagaaaa
      241 acaagataaa attgaagttg aatatagaat tgtaagaaaa gatggaagta taatttggat
      301 tttggagaga agtaagtcta taaatgataa taaaggaagt gagtgtattc agggagtttt
      361 aactgatata acagaattta agaaaataca agaagagtta gattcaaaaa gatttgaaat
      421 acaggctatc aatgatagta ttgttggtgg aatagtgata actgagataa atgatgattt
      481 tgatagtata tatttaaatg aaggttattt aaatatgatt ggatatacac gcaaacaact
      541 gaaagatgag ctaaataata aattaatcaa acttattttt aaagatgata gaacagaatt
      601 aattaatagt ataaacaaac aactcaaatt aagtgataca tttgcatctg aacaaagagt
      661 tgaaaaaagg gatggcacta ttatatggat tttattaaaa ggaagaaaga tgatttatga
      721 taatggaaaa tctgtacttt gttgtgttat tgtagatatt acttctgcta aattattaga
      781 gcaagaactt aaaaagagtg aagaacgtca tgaaatcatt ctaaagcaga cgcaaaatat
      841 tataatagaa tgggatatag aaaaagataa aatgaatata tcacctaatt ggtataataa
      901 gtttaaatat gaccctataa ctgaaaacgc aacaaaaaat ataattaaat cagtagatat
      961 atatcatgat gatatggata agtttatttc attaattaaa cttacttcac aaggtagtga
     1021 gtatgaagag tgtgaaatta gacttagaac tagagatact atttacgctt ggtttagtat
     1081 aaaggcaaca actttatttg ataataatgg taaaccttat aaagtagtag gaagtttgac
     1141 tgatattgat gaatcaaaac gagaagctga aaagttgaaa gaaagggcag aaaaagaccc
     1201 tcttacagat ttgtataata aaaaagcatc acaatcactt atagaagagt acataacatt
     1261 ggataaaaaa agaaaaggtg ctttaatgat tattgatatt gatgacttta aaggaataaa
     1321 tgataattta ggacatttat atggggatgc tgtattaagt gaagttgcaa atgacctaat
     1381 aaatctattt agagcaaccg atatagtagg cagaattggt ggagatgagt ttattgtatt
     1441 tataaaagat atttctaaaa caagagatat tataaataaa gcagaagaat tggtaaaaac
     1501 atttgaaagg tcttttttag gtaaaggacg aaattataaa atatcattaa gtgtaggaat
     1561 tgctaagttt ccacaagatg gtatggacta tatgacacta tttagaaatg cggatatagc
     1621 tctttactca gccaaaggga agggcaagaa ttgttataca ttgtatgata aaaaaattga
     1681 taaagtccaa tatataacta atacaagaaa tgcaggagat agtgataatg tagagatgtc
     1741 ttttaaagaa catatcacaa attatatatt tgatatacta tatgaatcaa gtgatataga
     1801 agtggcagta aatttaattc taagtattgt aggtaaacac tttaatgtga gtcgcatgta
     1861 tatatttgaa aattcagatg ataatcgagt atgtaaaaat acatttgaat ggtgtaacga
     1921 aggtataacc tcggaaatta ataatcttaa aaatatttgt tacaaggatt taggcaacta
     1981 tcttttaaat tttaatgcag atgggttatt ttattgtaat gatattgaaa atttaggtag
     2041 tgatgtgaaa aaagtactta agccacaagg catacgttca atattacaat gtaaaatttt
     2101 agagggtaat gaatttagag ggttcatagg atttgatgaa tgttcatata atcgtatgtg
     2161 gactaatgat gaagtgaaca ctttgacatt tatatctaaa acgctcaata tgtttttatt
     2221 aaaaatgcgt attcagagta agctagaaga gtctttaaca attcaaaaat ctgtgatgga
     2281 taatatggat atatgggcat atgttgttga caggaatacc tatgaattac tatttatcaa
     2341 taaaaaaatg cttgagtcag caccaaacac tcatgtaggt gatttatgtt ataaagcgat
     2401 atggaataat caagataaac cttgtaaaaa atgccctatg atatatttaa aagatggtga
     2461 tgatacttat aaatcattca tgcataataa aaagtttgag gtacaggcaa atataactac
     2521 aactactttg aaatggtttg atggtaaaga tgcatgttta atatttggaa gtgaaataac
     2581 tagtattgaa gctgaagaaa atacttaaaa tactgtgtta tatatatact taaaatcgca
     2641 atttgaattt attagactta aaatgtctat ataaatcatt taagttgtgg ttttaagtat
     2701 tttttatgat ataattaagc gttaagaaca aaattaaaag atacttttgt ataaaaaaat
     2761 atttgttaaa tatatattga gcaggtgata atatggaaaa taagttttta ccaataagca
     2821 aacaagatat gatagataga ggatgggaag aattagattt tgttctagtt acaggagatg
     2881 cttatgtgga ccatcatagt tttggaacag caataatatc tagagtttta gaaagtgctg
     2941 ggtataaagt tggaataata gctcaaccag actggagaac tacagatgat tttatgaagc
     3001 taggaaaacc tagacttgca tttctagtta atgcaggaaa tatggattca atggtcaatc
     3061 attattctgt aagtaaaaag catagagaaa aggatatgta ttctccaggt gggaaaatgg
     3121 gttatagacc agatagagct actatagtat attgcaataa aataagagaa gcatacagtg
     3181 atgttgcaat agtaataggt ggaattgaag cgagcttaag aagatttgca cactatgatt
     3241 attggagtga taaggttaga aagtctatgt taatagatag tggagcagat ttattagttt
     3301 atggtatgag tgaaaagcag attgtagaag tagccaatgc acttaatgat ggatatgacc
     3361 caaaatacat tagacatatt gatgggacat gctatatttc agatacattg gaagaaatat
     3421 atgataaata tatattaatt ccatcatata aagaaatttg tgaagataaa atgaagtatg
     3481 tagaagcttt taaaattcaa tatgatgagc aagacccatt tagaggtaat ataattgttc
     3541 aacctcatgg aagtaagtat ctggttcaaa ataagccaga aaaaccactt agtagagaag
     3601 aactagatga agtatatgga ttaccatatc agaagacata ccatcctgtg tatgaaaaat
     3661 ttggaggaat accagctata gaagaagtaa aatttagtat agtaagttca agaggctgtt
     3721 ttggtagttg ttcattttgt gcaataacat ttcatcaagg tagagcagtg caaagtagaa
     3781 gtgaaaaatc aattattgat gaagcaatag gaataacaaa tttagatgat tttaaagggt
     3841 atatacatga tgttggaggt ccaacagcta attttagaag accagcatgt aaaaaacaaa
     3901 ttactaaagg tgcttgtaaa aatagacaat gcttatctcc atcaccatgt aaaaatttag
     3961 atgcagacca cagtgaatac ttacatttac ttagagctgt aagaaaatta cctaaaataa
     4021 aaaaggtgtt tgtgcgttct ggaattagat acgattatgt aatggcagat aagaacaata
     4081 aatttcttag agagttgata gagcatcatg taagtggtca gttaaaggta gccccagaac
     4141 atatatcaga agaagtttta aagtatatgc aaaaaccagc aggaaaaact tatgataaat
     4201 ttagacagaa attttttgct ataaatgaag aattaggtaa aaagcagtat ttgataccct
     4261 atttaatgtc tagtcaccct ggttctacct taaatagcgc tatagagtta gcagagtatt
     4321 taagagatac tcactaccaa ccagagcagg ttcaggattt ttatccaact ccagggactc
     4381 tatctacaac catgttttat acaggtattg acccgcttac aatgaagcca gtttatgttc
     4441 ctaaatcaaa acgtgacaag gcaatgcaaa gggctttatt acaatataga gcacctagaa
     4501 attatgattt ggtatactct gcgttagttg aagcaggaag agaagactta attggttttg
     4561 gacatagatg tcttataaag ccaaaaaatg aaaagcctta ttttaataga aataattcta
     4621 aaaaaaatgt atctaaagga acaaataaaa acaaaaaaac taatacaaat aacagaagta
     4681 ataaaaatca acaaaaaagt tcaactaaaa aaaagaaaaa acattaaagt ataaataaaa
     4741 atacttaaaa aagaaggatt attcgataaa ttaacagaaa ttagtataaa taaatataat
     4801 tattttaatg acgttacctt tgggaggaag agatgaaaaa gtatatttct ataatattat
     4861 tagtggtgtt gactatggtt gttttggtag gatgttcacc aggaaaagat aaccctaaaa
     4921 ataaggagct atcagttgtc aaacaagtaa aagtagctgt tccagatgga ctaccagcag
     4981 tggcaatagc taaacttgca aatgaaaatc cagagattaa agaaggatat gaaacagtgt
     5041 atagcattga aaagacacct gaagcgattt ccactagagt gatgaaaaaa gatgcagata
     5101 tagctatagt tccttcaaac atggctgcaa tagcgtataa taaaacttca tcatataata
     5161 ttgttggaac tgtaggaatg ggttcacttt atttggtgtc aacagaaaat ataaaagatt
     5221 actctgattt agttggtaaa gaagttggat gtactggaaa aggacttact ccagacataa
     5281 ctataaaaag tcttttgtta caaaaggata taaattattc agatataaaa tttaattatg
     5341 taaattcagc aagtgaactg gtaccattat tggctactgg taaagttaaa acaggtattg
     5401 ttccagaacc tgcacttagt gctttgatgt ctaaaaatcc agacataaaa attataaaaa
     5461 gtttaaatga ctcatggaag gaagtaagtg gttcaaaaga tggttatcct caatctactt
     5521 taattgtaaa gtctgaattt ttaagagata ataaagattt tgttgattct tttgtaggtc
     5581 aattatcaaa tagtatagat tgggcaaaca aaaatcctga agaattaggt gcatattctg
     5641 aaaaaataaa aatatctact gaatcaaaga taataggaaa atctcttgaa agagctaatt
     5701 tgaaatatat tccagttaag gacatgatta aagattataa aaattactat gagaaattag
     5761 ctaattttga tgataagaca ttaggaggca aagtaccaga tgaagcaata tactttgttg
     5821 agaaataaaa aaaataaaga acaatttata gataaaatgg aggtaacact ttcttgtgtt
     5881 atcctcttat ttttatggca aattattgct ttaaaaattg ataatgatat ttttttacca
     5941 acagtaggtc aagtgtttaa tagtataaaa gagattatgc taaattctag tttctatata
     6001 gatatacttt actctatggg gagatgtttt tttagttttt tactggctat gatttttgct
     6061 attatacttt caatagtgtc ttatttaaat agatttttta gaaacttatt aaagccaatc
     6121 aatgctttga cacgttcaat accaactatg atattggtag tacttgcact tatttggttt
     6181 gaaaaggata gtaccccttt tatagttgga tttactatag tttttcctat attgtatgat
     6241 aacgttttgg gcgctatatt gaatatagac aagaacttat tagaaatggc taatgtgtat
     6301 aaaataaggt ttatagataa ggtgttaaaa atttatttac cagccataaa atttcaaatt
     6361 atatctatac tagtatcaac attctcttta agtttgaaag ttgctatagc aggagaggta
     6421 tatagtcaac ctacatatgg tattggaact atgatacaga ctgaaaaaat taactttaac
     6481 acatctggaa tatttgcatg gataattatt gttcttttaa tatctgctat tttacaaata
     6541 gctcaaaagt ttttagcaag gagagctttt ttatggaaaa gataaagttg agtattgaaa
     6601 atataaataa aagatatgat agtcgtatta tatttagaga ttttaatatt gatttttatg
     6661 taaatgaggt aaattgtatc cttggaaaat ctggttgtgg aaaaactaca ctcttgaaca
     6721 taataagtgg gattattaaa aatgacacca acaatctaaa tatcaaagaa aatttaaata
     6781 gagtaggaaa taagcttgaa gctagttaca tatttcaaga tgatagactt atagactggt
     6841 taacagtaga agaaaatata aagatagttg taaataaata ctataataaa actcaattaa
     6901 ataaaatttg tgatgaatac ttggaattgg ttggaattag tgattataaa aaattctatc
     6961 ctcaaatgct tagtggaggt attagacaaa gagtaaatat tgctagagct tttatatatc
     7021 catcaaaaaa cattataatg gatgaacctt ttaaatctat tgatgcaaag aatacacagc
     7081 ttataatgga taatttcaga aatatattaa gaaaagaaaa gagaactgtg ttatttgtaa
     7141 ctcataatat tgaggaagca ttgtttttag cagataggat atttatttta ggagactctc
     7201 caattagaat aaaaaaaata ttaaaaaatt ctaaggaatt ggaaaaaaat gaagttttga
     7261 agttgatata gagttataat aatactgtcg ttttaaaata tatttggtta aaatctataa
     7321 gactatatta aaatagtaca gaatattcaa aactgtgtat aatgttcgta ttttataaaa
     7381 aggaagtgaa tgtatggtta agactaatgc tatgagaata ttagattcaa ataagattga
     7441 ttataaagta atgtcttatg aagtgaaaag tgaacatgtg gatggtgttg aggtggcaca
     7501 tgatatagga agagatgtaa atgaagtgta taagacatta gtaactcaag gagttagtaa
     7561 gaatatatat gtatatgtaa taccagtaca tgaaaactta gacttaaaaa aggctgcaaa
     7621 agtagctaaa gagaaaagtg tagagatgat tcatgtaaaa gatataaata agcttacagg
     7681 ttatattaga ggcggatgtt ctcctatagg tatgaaaaag ctttataaaa cttttgtaaa
     7741 tgaaagtgca aagaatttag atacaattat tgtgagtgca ggaaaaatag gatatcaaat
     7801 agaactatct ccatttgatt tacaaagact tataaaagta gaatttgtag atgtaataaa
     7861 aaaataagaa ttacatataa aatatagagc ttatattctc aataataggt gttgtaatat
     7921 taataaaatg aataataatt ttaaaataaa tattcagtta cttatgtgta attggtataa
     7981 aaatattgag aaattttaaa aacaatggta aagtgtaagt ataatcacga atatttttta
     8041 taaagtaatg gagggatgtt cgcttgagaa aaataaaatc tgaacaaatc gtcgagcagg
     8101 taaaaaaact ttgtatagaa gcgagcctat atttaggaga agatgtttta agttgtataa
     8161 aagaaaaagc gaaaagcgaa aagagtgaag ttggaaaaaa tatattgaat atattagttg
     8221 aaaatgctga aatagctaaa gaaaaaaata taccaatatg ccaagatact ggtatggctg
     8281 tattttttgt tgaaatagga caagaagttt taattgaagg agacacactt actgatgcta
     8341 taaatgaggg agtaagacaa gggtatgaag aaggctactt aagaaaatct gttgtaagtc
     8401 caattaacag agtaaataca aaagataata cacctgctgt tattcattat gatatggtaa
     8461 aaggtgataa aattaaaata gaatttgcag ctaagggatt tggaagcgaa aatatgagta
     8521 agatgaaaat gttaaagcca tcagatggat tggaaggaat taaaaaattc ataatagata
     8581 cagtttcaga agctggacct aacccatgcc ctcctatggt aattggagtt ggtataggtg
     8641 ggactgtgga caaatgtgct caaatagcta aaaaggcact ttttagagaa ctaggtgaat
     8701 ttaataagga cgaaaatata gcaaaacttg aaagtgaact attaacagct attaataaac
     8761 ttggtatagg acctcaaggc ttaggtggta ctacaacagc tttgggatta aacatagaga
     8821 cttttcctac acacatagca ggtctacctg ttgttgtaaa tataaactgc catgcatcaa
     8881 gacataagaa ggtagtaatt taggaggaaa ataaaatgat aaaaataact acacctgtaa
     8941 atgaaattga tatagcaaaa ttaaattgtg gagatacaat tagtttgagt ggtatacttt
     9001 atacagcaag agatgctgct cataaaagat tgatagattg tataaataaa ggtgaagaat
     9061 taccatttga cgtatatggt caaggaatat attatgtagg acctactcct acaaagccag
     9121 gagaagttat aggtgctgca ggtccaacga cgagttatag aatggatgat ttgactatac
     9181 ctcttttaga aagaggtctt agattgatga taggcaaggg aaaaagaagt gatgaagtta
     9241 tagaaggtat gcaaaaatac ggagcagttt atcttgctgc aataggagga gctggagcgt
     9301 atatttcaaa ttcaataaaa tcatgtgaaa ttattgcata tgaagattta ggagctgaag
     9361 ctataagaaa gattgtagta gaagacctta agcttacagt agcaatagat agctatggga
     9421 ataatatata tgagcaaggt cgagcaattt atgaatgtaa ataatgttta gaaataaata
     9481 tgaatatcaa aattaaaaat agttgtgtat attttaagga ggaaaattga tgtctaagga
     9541 ttatgcaaaa ttggcattag aaatgcatga aatcaacaaa gggaaagtaa gtgtagaaag
     9601 caaggtagaa gttaaaacta aggatgattt atcaacagca tatacaccag gggttgcaga
     9661 accttgttta aaaatacatg aaaatcaaga tgatgtttat agatacactt ctaaagggaa
     9721 tctagtagca gtagttagtg atggaagtgc tgtacttgga ttaggtaaca ttggtgctga
     9781 agcatctata cctgttatgg aaggtaaagc tatattgttt aaacaatttg cagatgtaga
     9841 cgcttttcca atatgtttaa agactaatga tgtagatgag atagttaaat ctgtagagtt
     9901 gatggaacct gtatttggag gtataaattt ggaagacata tcttctccaa gatgttttga
     9961 gatagaagag agattaaaaa aatctttaag tatacctgtt tttcatgatg accaacatgg
    10021 gacagcaata atagttgcag cagccataat aaactcaata aagttaattg aaaataaaaa
    10081 aatagaagat ttagaaatag ttataaacgg agcaggagct gctggtatag ccatagcaaa
    10141 gatactactt aatatgaatg ttaaaaatat aatcttatgt gatagaagtg gagcattgga
    10201 agctagtata gaaaatctaa actatgttca aaaagaaatg ttaaaagtaa caaatataag
    10261 aaatgaaaaa ggacctttaa aagatattat aaaaggaaaa gatgtattta ttggtgtttc
    10321 aggtccagga gctgttacaa aggaaatggt tgaaagtatg tctgagaagc caataatatt
    10381 ggcaatggca aatcctgctc cagagataat gccagaagaa gcaaaattag gtggagcatt
    10441 tataatggga acaggtcgtt cagacttccc taatcaagtc aataatgtat tggcttttcc
    10501 tggaatattt agaggagcat tagatgttaa ggctaaagag ataaatgaag aaatgaagat
    10561 agctgctgca tatgcaattg ctaatacaat aagtgatgag gaaatatgcc cagagtatat
    10621 attaccagat gtatttaata agaatgtagt aaaaaatgtt gctaaggcag ttaaagaagc
    10681 agctataaaa actaaagtaa atagaatata aatttttata tgctcatata ttcttacttt
    10741 tatatacata tctttatgat taaacgtgtt agaaaacgta gtaaatttat actatcagac
    10801 tttaccttta ttacttaaaa tgatataata tgaatattat aaattaataa atttaaattt
    10861 attgaggtgc aagagataat ggaatataaa gttaatgacc aagaacttaa tgcttcaatg
    10921 tttatcccat tcgttaacag gatatggcca ggagattatg atacggataa aacacagact
    10981 gcattatcaa aaactttgaa tataactgct tatgatgata aagtgcttgt aggatgtctg
    11041 cgagttcttt cagatggtta ttattttgga acaattacag aattgcttgt tcttccagag
    11101 tatcagaaac aaggcattgg tagtaagctt ttccagcttg ctaaagagaa tacaccaact
    11161 atgctatatt ttggtgcaca gccaggagta gaagagttct atgaaaaaaa tggatgccaa
    11221 agaagtcttc aatcttacat aataaaaaag aaagatagtt attaaagttc cagctttcaa
    11281 taatcttaaa agtacaatat tttagtggaa tcatttcatt ttttattact aatatgacat
    11341 aaacatgtca tattagtatt gatatacttt taatatggtt aagagccaaa gaaaaatata
    11401 aggagtgatt taaaatggaa gaaactaaat tttgccaatg ctgtgcaatg ccaatgggac
    11461 aaactgatga attatatggg acaaataaag atggaagtaa aagtactgat tattgtagtt
    11521 attgttttaa agatggtgag tttacagctg atataagtat ggaagagatg atagaagttt
    11581 gtatccctca tatgcttcaa agtaataagg atatgactga agatgaagca agaacaatga
    11641 tgaacaattt ctttccaaca ttaaaacgct ggaaataatt ttatgcaaaa ttagttatat
    11701 gcaaaacatg aaatatggtg ttgagaataa ggggtggtgt gctttatagc ataccacctg
    11761 ttttgttact tgctagttca ggtattcttg tatgcttaaa tatgttctca tctctctttc
    11821 tattttaaat tgtaaaaatc taaaatatgt tatttatatt ctacttcttt atccatgtct
    11881 ttttctacca caagtcaaat ccaactacaa aatcatcatt ttcaattcac tgtttaatag
    11941 aattaacaga taaagagtta taaaattttc tattttttat ataaaataat acaggtcaag
    12001 ctgacttcta gctacttggg atagtgtata tatttatgat agttatagga gtaataccaa
    12061 tcattagact gttgatttgt gtagtctttt gtaaaaacat ttgttttaat atattgagtt
    12121 tttgaaaaag taaaattata attataataa attaagtgta ataatgtatt agaagtaaaa
    12181 ataattaaaa tagaggtgat taaatgagta gtatattgtt agttgaagat gatttaagct
    12241 tgatatatgg attagagttc tctatacaaa aaagtggatt tagtatagac atagcaagga
    12301 cagtaaaaga agctatgcaa atgtatgagg aaaaaaatta tgacttattg cttttagatg
    12361 tatccctacc tgatggtaat ggatttcaga tttgcgaaaa agtacgagag gtttctaata
    12421 tacccattat atttttgact gcttcagatg aagaggtaaa tgtagtgatg ggtcttgata
    12481 tgggtggaga cgattatata acaaaacctt ttaaattaaa tgaattaatt tcaagaataa
    12541 aagcattact tagacgttat aatgttgcaa gtaatgtgaa tgaattaagt tcaaataata
    12601 ttactattaa attacttgaa aatcgtgttt ttaaaggtaa atttgaggtg gaacttacca
    12661 cagcagaata taaattattg tgcttattta tgaaaaataa aaatattgta cttactagaa
    12721 aaaacatatt ggacaagtta tgggatggaa atggtagctt tatagatgac aatacattat
    12781 cagtatatgt aagacgattg agaaataaaa ttgaagataa tcctgaaaat cctaagtttc
    12841 tattgaccgt tagaagaatg ggttataagt ggaatgtaat taagtgaggg tattaatatg
    12901 aagatatttt ctaataaaga tataaaaata ttttttttaa tgcttatatc tattttagta
    12961 atgtttatta tagtagggca aatagtggtt ataagtataa ctaacaatta taaaaaaacg
    13021 ttgatagagc atgattataa tatagctgga tacttatata gtataggtgt tgataaatct
    13081 aaaataccat cagcatttgt tgcacatgaa gataaatata ttttaaaagg aaaagaaata
    13141 ctagatacag ctggatatgg atttgatata gatgttagct ttacaccaag tgtaaaattg
    13201 tttcaaagta aatatataaa aactatgtct atctatttat tagtattttt tactatgatt
    13261 atttttatac tatatatgta tttttttagg caacaaagta agatagaaga ggctagttat
    13321 aaaattgatt tatttatgaa tggaaatata aatgaaagac taaatacata tgaagaaggt
    13381 agcttatcaa aattatttag ttcaatagat gttatgtcga catctttaaa tacacatata
    13441 ttaaaagaaa aacagagcag agaatttctt aaaaatacaa tttcggatat atcgcatcaa
    13501 ttaaaaactc cacttacagc tttaataatg tacaatcaga tactacaaga agaaagtcat
    13561 aatagtgatg tcgttgaagc ttttgtacaa aaaagtcaaa gtgaactaga acgcattgaa
    13621 acattgatta agaatttatt aaagattaca aagatagatt caaaaacaat aattctcaat
    13681 aaaaataacg ttaatcttaa aaatttaatt gtcaaaattt tgtatagctt tgaaactagg
    13741 gcagaaaaag aaggaaaggt tatttctgta aatggttcag aaaatacatt gttgttctgc
    13801 gattatgaat ggatgtcaga ggctttaagt aatcttataa agaatgcatt agataataca
    13861 aaagagaatg ataaaataac gatagagtgg tttgagacga ctataaccac cattgtttca
    13921 gttaaagata ctggaaatgg aatccatgtt gaggatattc atcatatatt taaaaggttt
    13981 tatagaagta agttttcaaa agataatcaa ggtttaggac tgggattacc actagttaaa
    14041 tctattgtgg aacaacataa tggtactatt tctgtagaaa gtaaactatc acaaggaagt
    14101 atattcacca taagtttttt aaagcttact aatatgtaag ataaaataca tcttgcagta
    14161 aggtatatag attattatta tattaatcaa ttagaaaggt gagagagaaa atggatattc
    14221 ttgtggttaa taatttatgt aaaacatatg gaaatggata tacaaaggta gaggcattga
    14281 aagatgtatc ctttacagtt tcaaaaggag agtttgtgac tgtagttgga gaatctggtt
    14341 caggaaagag tacactatta aatctcttag gaggtcttga tactccaaca tcagggagaa
    14401 tatttattga tggtaaagaa atatgttcaa tgaaagagga aaaacagact atttttagac
    14461 gtcgtaacat agggtttata tttcaggcat ataaccttat accagaacta actgtggagc
    14521 aaaatataat attcccattg ttattagatt ataaaaaacc agatactatt tacttagatg
    14581 aagtattgac tattttaggt cttaaagata ggaaaaatca tcttccaaat caattatctg
    14641 gtggtcaaca acagagagtt gcaattggaa gggctttgat tacaaagcca agtattatac
    14701 tggcagatga gccaacggga aatcttgaca gtaaaaatag taatgatgta atttcacttt
    14761 taaaagtgac tgcagaaaag tataatcaaa ccattattat gataactcat aatacgaata
    14821 tagcttctac agcagataga atactacagg tttctgatgg tgtgcttaca gacctggggg
    14881 tgaatagaga atgagaagct acttagaatt agtaccacaa tatgcaaaag tacataagaa
    14941 aaaaaataga actacaattt tatgtattat tattgcagta tgcttagtta ctgctatctt
    15001 tggacttgca gatatggcta tacaatgtca aaagatacaa tttattaaag atgatggaaa
    15061 ttggcacata gtaattagaa atattgatga aaagtttata tcagaaataa atagtagagt
    15121 agatatagct caccatggta gggggcaagt cgttgacaat ggagagttta aatcaaagga
    15181 gattttgata caaggtttag atgaagaagt agcaaagcaa tttaacttag aaataataag
    15241 gggtcgttat cctattgggg agaatgagac tttaatagat gaggatgcag ttaaaagctt
    15301 taatctaaaa ataggagatt ttatcaaaat aaaattagca gatggtagaa ataaaaatta
    15361 taagatagta ggtgaatatt catcaatatc aagcttaaga gctacagata aacatggatt
    15421 aatactttca atattgggta cacaagctat taaaaaatca gaaacgtacg attattatta
    15481 tattacattt aaagaaaagg ttaatattag aaaagccact tctgatatta aaaatacctt
    15541 caatttaagt gataaacaaa ttggtaaaaa tgaaagatta ctttctatta taggtcaagg
    15601 tgactctgac ttttctaaac cattgtattt aacagcagga ttactttttg tacttgtttt
    15661 agtagcaagt attatcatga tatataatag ttttaacatg agtgtaattg agagagttaa
    15721 attttttggt ctgttacgtt gtctagggtc atcaaaacat caagttaaaa aatttgttgt
    15781 tttagaaagt ttaattttaa gtataaaagc tattccaata ggcttagtat taggaagtct
    15841 tgtaactatt atatcttcta tatttcttaa gtatgttaat gagttattca gctcaatgcc
    15901 aattttaaaa gttagtttta ttggtattgc atttggaata attgtagggt ttttaactgt
    15961 aatattatca gctattattc cagcaaaaaa agcttcccgt gtatctccac ttagcgctgt
    16021 acgtgggaat ctaatgtcca aagaaatttt aaataacggg aagacaagta aagttgcaaa
    16081 tataggaagt tttaaaattg agacttctat gggattaaat catgcttttt caagaaagaa
    16141 atcttttgta cttatgactt cttcatttgc cattagtatt atactatttt taggttttag
    16201 tgtgcttata gaccttatgt atcaaggact taagcctatg aagccagaag ctcctcatat
    16261 atctattgtt gaccctaatt ttaagtctgt tttaactaag gaagatatta atgaaataag
    16321 caatatcaaa ggagtaaaga aagtatacag tagaatgatg acggatatag aagctacata
    16381 taatgggaag aagacaaaat cacaattaat ttcttatgaa aagtatcaat ttaattgggc
    16441 aaaaaaatat ctattaaaag gagaagttaa tgaaaataaa ctacagtcag aaaaccttgc
    16501 attagtagaa gaggggcatg gatttgatat aggagataag atatatatta aaaatggaaa
    16561 tgctgaaaaa gagttagagg taggaggtat actttcctca attccatttg aggctaaaga
    16621 taaatatatt ggaaatatta taacatctga aaatactttt acgaatataa caggtataaa
    16681 gggttatata ataatggata ttcaggttga taaagatact cctggagatt taattgaaaa
    16741 tataagacgt gtagtagatg ataacttaga ggtcagagat aaaagacaaa gtaacttaga
    16801 ggttaaaaat tcgttttata ctatggcaac atttgtatat ggatttgtgt ttattattgc
    16861 gattataagc atgtttaata tcataaatag catgaatata agtgttacaa gtagaaagaa
    16921 ttactatggt gttatgcgtg cagttggcat atccaacaga caattaagaa gaatggtaat
    16981 agtagaggca ggttcatatg cagtgagtgg ttgtttatta ggtagtatac taggattaat
    17041 attacatagg tatatgttta tatcgttagt aagctcaatg tttcatttag agtggcaagt
    17101 accttttaat ttactactta tcactgtact aactatgata ataataactt tattatcagt
    17161 aagaaaacca atcaaaaaga tttgtgaatt agatataata gactcaataa atgcacaata
    17221 aatataggta ttgatttaaa ttaaaaaaac gaagtaaaaa tgaaatagaa aatatttttc
    17281 atttttactt cgttttttat attataaaat ttttgttatg ttacaaaatt tctataaaga
    17341 tgatgtgaat ctaaacatta tatgacaacg tgagcattta agttaaaata ttttaaatat
    17401 actattttag ataagttccc tcttcatctt gataaattaa tccttttttc ataagcactc
    17461 caagagctcg tttaaaattt ttcttacttg aattaaataa tatagaaata tctttagggt
    17521 cagatttatc attaaatctc atatatccat cagaaccttc taaataactt aaaatcttat
    17581 tttctaaagt atcaagttca tcctttctat ttcctctagg agaaagacca agttttccat
    17641 cttcatatat ttttatgaca tgtaaatttt caagaacatc accagttttt agttcagtaa
    17701 agtactcatt atgaagtatt acaccagcat atttattgtc tacacatatc attgcagaat
    17761 tattagtttg gaatccatat acaactccag ttacaatatc tcctacattg taagtatggt
    17821 ctgttaataa gtatgaatct atatctgtag tagcagcaat tcttttgctt ttgtctaaat
    17881 atatataaaa taaatatttt tcgtctctaa atagtggata agtcttagct ttaaaaggaa
    17941 ctaatatgtc tttaggaagt cccatatcta taaaagtacc aatgtctgta ttatcaacta
    18001 cttttaaatg tgctacatcc ccaaccttag ctaaaggcgg tattaaagtt gcagcagttc
    18061 tgtcatcaga atctttaaat atgaaagcat ttacttcatc acctatgttt atttcatttt
    18121 ttccgattaa tctattatga agaagtatat cgtcttttgt gttattagtt tgtccatcca
    18181 aaaaatatcc aaattcagtt tttcttttta ctgtaagttt attaaaatca cctatgttta
    18241 tcaaattaaa atcatcctct cattttatat ttataattta gccataatat acttatgtag
    18301 tagtttgtca cagtatctat attagtatta gtgaaattta caataaataa tatagttatt
    18361 tatgtatttt tcaattctct agcttcttat atagtataaa ctccaatttc ttttttgtct
    18421 ttaggtttta ttaaaatttt tacaaaataa gttaaaaagt attgaaataa aatttctttt
    18481 aaagtataat tggtatatac caaaattggt atataccata tcgaaaagtg gtgactatat
    18541 gaaaatagta aataaatcta gtaatatacc attgcatact caattaagtt caataataag
    18601 agagatgata gaaactggtg agcttaaaga gggcgatgca ataatgccag aaagagaact
    18661 ttgcaacata caaaatgtaa gtcgtatgac agtaaataaa accattgttg gattagttac
    18721 agaaggactt ctttataggg tacaaggtaa agggacattc gttgcaaagc aaaaaaagaa
    18781 gtatcagttt tcaaatgtaa aaggttttac tgatgttatg aaagaaaagg gagtaaacat
    18841 aaaaacggat atattatcct ttgagatgga actaccagat gatttagtta aaagaaagct
    18901 tggaataagt gataatacta caaatatata taagattgtc agacttagat atactgatgg
    18961 agagccattt ggattagaaa tagtatatct atcagaagaa atgtgtaagg gtctaacaaa
    19021 agggatattg gataattctt cattatatag ggttttaaat gaaaaatatg gctataagat
    19081 acaaaaagct gaacaggtaa tggagccagt aatactttca gatgaagaaa gtaagcttct
    19141 tgaaacagat gaaggtgctt tagctcttaa acttcataga aattcctata atagagaagg
    19201 tagacctata gaatatacca tatcaatatt tagaacagat aaatatcaat atgaaatagt
    19261 attgagtgag taggtgaata atatgaaatg tttaattaat ggaaagataa tattaaaaaa
    19321 tcaaatctta gaaaataaag tattagtatt tgatgaaaaa ataattgata tagctgatag
    19381 tgtaccaaaa gattgtgaag ttatagatgc tgatggaaaa tatatatctc cagggcttat
    19441 tgatattcat atacatggaa acatgggaaa agatacaatg gattcgactg atgaatctat
    19501 tgaaacaatt tcaaaatcaa ttatgagaca tggagtaaca tcttttcttc caactacaat
    19561 gactatggat aaagaacatg tttatgatgc acttgaagtt attaaaaaag cccaaaatag
    19621 aaagcttgaa ggggcacaag tattaggagc acatttagaa ggacctttta taaatgagaa
    19681 ttataaggga gcacaaaatg aaaaatttat aataaattca aaatacgaat ttataaaaga
    19741 atataaagat gttataaaag ttataactta tgctccagaa aaagatattg attttgattt
    19801 tactagagaa ataaaaagat gtacagatat agttttatct attggtcata gtaatgcaaa
    19861 ttatgaccaa gctaaagaag ctataaattt aggagtaact aatgtaactc acatgttcaa
    19921 tgcaatgact ggtttaaacc atagagaccc aggcgttgtt ggtgcagcac ttacaactaa
    19981 tgtgtacagt gagttaatag cagatacaat ccatataaat aaagatttat ttcaatttat
    20041 actaaataat aaaggaaaag aaagactgat tttaataaca gattctatag aagctggtgg
    20101 tcttgaagat ggcaattatt ctcttggagg tcagaaagtc atagttaagg gtaatgaagc
    20161 tagacttgaa aatggagcat tagctggaag tgtgttgtct ttaaataaaa tggtatttaa
    20221 cttcttaaat aatacaaacc ttaaagtgaa tgaagcaatt aatttagctt ctttaaatcc
    20281 agcaacatct ttgggaataa atgacaaaaa aggaagctta gaaataggta aggatgcaga
    20341 tatagctgta tttgatgaga atttagattg taaaatgaca ctttgcttgg gggaagtagt
    20401 atataaaaat atatagtata caaaacaaac cttaaagaca gtaaaaacaa tataaaaaat
    20461 aataaattaa acttacatta gtaaaagtag tagttaaaat ttaggaggaa taagatgaga
    20521 gtattagtgt gtaaagatta tgatggaatg agtaaaaaag ccgctgaaat gatagcagct
    20581 caaatagttt taaaaccaaa ttcaatactg ggactagcta caggaagtac accagtaggt
    20641 atgtatagag atttagtaaa aaaatacaat gataatatag tagatttttc agatgtgatg
    20701 agctttaatt tagatgaata ttataagtta ccaatatcta atgaccaaag ctatgattat
    20761 tttatgaaag agaatctatt taatcatgta aacataaagc cagaaaatac tcatctacca
    20821 aatggaatgg ctgatgatat tgaaaaagag tgtatgaact atgaagcttc tatagatgca
    20881 gctggtggta tagatgtaca agtacttgga ataggtcgta atgctcatat aggttttaat
    20941 gaacctgata ctaaatttgc aaaaagaact catgtagttg aacttacaga atctacaata
    21001 gaagctaatg caagattctt taaatcaaga gaagatgttc ctaaaaaagc tgtaagtatg
    21061 ggaataggtt caatattgaa gtctaagaaa atattgcttt tagcttcagg agaagaaaaa
    21121 gctgatgcag tatataatac tgtttacgga gatataactc cagaagttcc tggttctata
    21181 cttcaacttc ataaagacac tatagttata gtagatgaag cagcagcaag taaattgaat
    21241 ccaaaagatt ataaattagt ataagaatag aatgtagttt aaataaaaaa tagctatcat
    21301 tgaataatca aatttattta atgatagcta tttttattta aattagttat tattgtttaa
    21361 attacttgct tattactttt tacgaaaact cgttatcaag aagtatccaa ataagatgtt
    21421 aaaaattatt atgtttagtg ggattctatc aagcataata aaattaccta taattgggat
    21481 attaccaact ggaaacataa agaagaatac aggcatcagt gatagtaaaa aagatggaaa
    21541 acaaagaaga agtaatcttg gaatatcaat ataccaacta ccatcctttt tgtattctct
    21601 aatcaataat tcagttccta gtaataaacc aataccacca gaggaaatta aattaataaa
    21661 aatgtatctt ttaaagtctt gcatataacc agtgtaaaat tgagtataga aatcataatt
    21721 taatttaacg gctaaaaaat acataagcaa tagaatgatt aaaaataaaa gatatctaag
    21781 agcttttttc attttaaatt ctcccttcag aaatacttta tttagtttta ttttgttgga
    21841 atctgatttt atcatttagt ttaagaaatc cataatatta gtgaagaatg aaataagtat
    21901 tatatatgaa taaatatttt tagaattaag gtgaattgta gtgcttaata taactataat
    21961 attaattgtt tgaatattca ataaaaagtt cgaaaaatat caattctgat ataaactgaa
    22021 taaatacagt tgaatattaa attattattt tatcaaaaat atgcaaagta ctacaccttc
    22081 aaatatcacc taaagtaatt aaaaggcaat taaagtgaag tagttcaagt atttctaaaa
    22141 taagtttaat aaattctata ataatttatt ttttgcctta aaattgcctt aaaatccaaa
    22201 caattttaaa actaatgtac tataaaaagc tggtataatt taaatatagt aaaaaataag
    22261 atttatctta tctattggga tttaattatt taatcaatat atttatacca tttaatttaa
    22321 ataaaaagtc tattaaggag tgagcttatg agtaagttaa tttatatagc agatgatgaa
    22381 gataatataa gaaatttggt aaagacattc ttaaaaaatg aaggtcatga tgttatggat
    22441 tttaaaacgg gtgatgaact tttagaacaa tttaatataa aggagtgtga cttagtaata
    22501 ctagatataa tgatgcctgg ctcaagtggt tttgaagtat gtacaaaact tcgtgagaaa
    22561 agtacagtcc caataatcat gcttactgca agagatacag atatagatta tattacaggg
    22621 ataaccttag gtagtgatga ttattttaca aaaccattta gcccaatgtc tttggttatg
    22681 agagtaaaat ctatatttag aaggatagag tttgaaaaga aacaaaatta tgataaatat
    22741 agtaactcga tagatatgga attaaaattt ggtgatgtaa taataaataa aaagaataaa
    22801 atcgttacaa gtaaaaatgt aaatatagac cttacaccaa atgaatataa cctattgact
    22861 tatttgtttg aaaatataga tagagcaatt tccagagatg aattattaaa caaaatatgg
    22921 ggatacgata tagaagttga gacaagggct gctgatgata cagtgaagag attacgtaaa
    22981 aaaattttag atacaaatat tttaatagaa actgtatggg ggtttggatt taggttgaag
    23041 gagaaaagtt aagatgaaga atggaaatga caagaataga attggaaaga ttaaatccaa
    23101 atttaaatta aagttctcaa agaaaaatat aagcataaaa actaggattt tgttgagtat
    23161 actttgtttg ataattatag tatttatgat aatattagta tcttttaatg tatttcttga
    23221 tacatatata aaaacaactg cgaatgaaga attgacaaag tctacagaaa tagtagaaca
    23281 tatggatagt aattttagac ctattaagcc accacaagat gatggaaagc ttcctcccaa
    23341 agtattatct aattttatga gaaatgtaca agataaagtt aggatggcag aaacacaatc
    23401 agatgctgat gctatggtag ttgattctaa gtataacctg atatttccaa caagagagga
    23461 cgatttctta aaaaatgttg atgagatgga gctgattagg gagtgtattc aaaatgaaaa
    23521 attagattta aattcagatg caaatacacg gatatctacc agaaataagg attattatat
    23581 ttcaactgta aaaattacca gtatatatgg tgaacaagac aagtatctta tactttttat
    23641 agacatatca aaaactctaa atctagcaca aaagataaat attgtattaa tatctgtaat
    23701 gtgttttgct ggaatattag caatatttac agctgtgatt ttatctgaga aaattgctaa
    23761 accgataaaa gagttgtgtg agtttgcaaa aaaaatgggt caaggagatt tcaaaagaac
    23821 ctactttgac ttttcagata aagaattagt agagctctca actgttatga ataagagtgc
    23881 tgagtatctg gataagtatg ataatgaaca aaaaatattt tttcaaaatg catctcatga
    23941 attaaggaca ccacttatgt ctattaaagg ttatgcagaa gctataaaat ataatgttat
    24001 agatagtaaa catgcaagtg atataattct tgaagaaagt gataggttaa gtgatatggt
    24061 tgaagattta ttatacattt ctaaaataga taatataact aaagattttg aacttgtaga
    24121 atgtgattta agagaagtac tttcaaactg tggagctagg caaaatgtaa gagctataaa
    24181 taaggggata aagtttattt atgaatttga tgaagaaatg gttttatttg aatgtgatga
    24241 aaaaaacatt tccaaagcat ttatgaatct tatagaaaat gctttgagat acgctaagag
    24301 tgaaataaaa atagtgtgta agtataatca aaaaaatata gttgtgataa tagaagatga
    24361 tggaattgga atcaagaaag aagatttacc tcatgttttt gaaagattct ataagggagt
    24421 aggaggaaat catggaattg gattatcaat agttaaatct attgtaaata agcatggtgg
    24481 gcgtatatac gtagaaaatg gcaaaaaagg agctaaattt actatattat ttaaattgta
    24541 aaaaaattag agaaaatata gagatgtttg aagttgtttt ttatatatat ctgttgtata
    24601 ctgttgaaaa aagaacaata ttaagctgac atatattacg atagttgaca tttaaagtaa
    24661 atgcttaaat atagatacga aaggaaaagt aaaatctgaa gtgtatttag gtttaagtaa
    24721 gcttcttatt ttataaaaaa tatgtatgaa agaacagctg atttttaata ttacaaattt
    24781 agtaacggtt gtatttgaag cttttgtcat ccatatgttt ttatcagact ttttaggcca
    24841 gaaagaaggg tatataaaag ttgtaagata tgctaaactg ggttttataa tatgtttagg
    24901 attctgtaat ttaataactt taaaccctaa aatcacaatg cctttaattt tcatccttat
    24961 tttttcaaca tcatttttat ataaaggaaa tttaaagaca cgattgttta ctactgtttt
    25021 attaagtata ttctttatac tgtcagaaat tgtagttaca agtatttttg ttttatttgt
    25081 aaaagaaggc tttgaaataa tgcttgaaaa taatagtata agagtattgg cgacaatttt
    25141 atctaaaatt gtctttttgt tgacttgtaa aattatttgt ctatttaaaa aagatgtaca
    25201 tttagatatg cccataaagt attggctccc tttattttta atacctatat ttagtttatt
    25261 tctgtctgtt tctatctttg atgtaagtaa attttttagt cttgaaagtt taaaatttct
    25321 aagcttaata tctagtgttg gtattttata tattaatttt attgtctttt atttatttaa
    25381 atttattata gataaaacta aactatctat gaaatatgaa ctacttgaaa aagaaataat
    25441 tcataaggaa gaattaagac ttagtaatga gtgttataaa attattgttg aacagactga
    25501 ttctgtcgtt ttcgaatgga atatcaaaga aaataaatca tttgtatcgc aggcttggac
    25561 tgaaaaattt ggatataaca atgcatgtaa gaatattttt aaagaaatta aagataaaga
    25621 cttagttcat tctgaagata aagctatatt tgaaggtttt cttgaaagta ttaaaaaaaa
    25681 gaatatgcac aatcaagcag tatatcgttt aaaaaaatct aatggtgaat atatttggtg
    25741 tagaacatca attacaagca tttataatga tgaaaatgaa cttttaagag ttgtcggagt
    25801 aattgtagat gtagatagtg atataaaaaa atatgaagag ttaagaacta gagcagaaag
    25861 tgactcattg accaatatat acaataaagg tacatttgaa aaattagtgg aagaaactat
    25921 agttatgaat acaggtgata aaaaagatgc attattcatt attgatttgg atgattttaa
    25981 agaaataaat gataattttg gtcatccatt tggtgatttt gtactaaaaa catttgctga
    26041 taagatacaa actagttttg gtagtaaaga cttagtcgga agaattggtg gagatgaatt
    26101 tgttgtatat atgcaagatt atgttacaga agtaaatttg cataaaaaag ctaaagaatt
    26161 aaatagagta ctttcggata attacactga tttgagtttt agttttgatg cttctgtcag
    26221 tataggtatt gcaaggtatc ctcaagatgg aacttcattt tttgaattgt ttaaaaatgc
    26281 agatagagca ctttatagca taaaagcatc aggtaaaaat tcatattgtt tatttgaaga
    26341 agaactatat gtacagtgaa tttgaattta gattttatgt gagagtaaga tttatatata
    26401 cttgtggctg tctgatttat aaaatatgtt actttgtata tttttatatg tttcagtaag
    26461 gttaattaac ataagcaaag gtgtaagtaa atttatatta gtttaagttt acttacacct
    26521 ttataaaaat acaaataata gattcattta cattcttaaa tgattaaagc tctttacctt
    26581 ctaaacatgc ataacatatc gttataattc cagtaaataa gactaaaata cccattccta
    26641 agattacagt ttcattatct attactccaa atgtttttaa agtaacttat ttcgctttgt
    26701 atcataataa tttgtctctt tttacatttt aacttctgct gagatttgag ttagatattc
    26761 gttagaatct cgcacactta tatcatatag aagatattct tcataaacaa aatccccaat
    26821 acgtaaatta ttctgattaa aaaaatttaa taacttttca taagccttgt aaatagtttc
    26881 ataactccct ttatgataag cagttacata aagtcccttt ggtttgattg atacaggata
    26941 tttcttgtac tctttattaa cttttgtaaa taaatatgaa tatgattcaa agtctttatt
    27001 aattatatct tcacctttaa tgattgagcc aatcgtacca taatcactca attcatagtt
    27061 gtccataaag ttaatacatt catataaaaa attttcttcc tcatctccta aaaactgttc
    27121 tttatatgta acaggtgttt tgaccaagta ctcttcttca tgttctttta aagtaatagt
    27181 attcgcatct atattacagg catttttagt gaatgaaata gtagattcca ttagagcttg
    27241 aatattattt agtttttcaa tttcattttt aatatcaatg gttttctttt ctaaaagctc
    27301 aattagtaaa ttaggtgttc ttttgtctat ataagcttta atttctttta aaggcatttt
    27361 tacttcccta agtgaagaaa ttataccaaa aatgctaagt tgatggtatg agtagtatct
    27421 atatccattt tctttcttta attctggtga gaaaagacct atatcatcat aatggaaaag
    27481 tgttttttta ttgattccac aaatctttgc aaattctcca gttgtaaaat aattttttga
    27541 aacttgactc ataattataa ttcctccttg actatcctgt tacagtatag tttatcatag
    27601 tattttgtaa aggagtgtga ttatatgaaa aaattaattc attttttaaa accatataga
    27661 gttttaattg ttgtagtttt gatatttacg tttttacaaa cacttggaac tttgtatata
    27721 ccaacactta cagcaaatat agtaaacaat ggtgtagtaa agggagatat agattatatt
    27781 gtaaaaacag gtttaatgat gatgatagta gctggtataa cagctttatc agctgtgtta
    27841 gtttgtaaag tatcagcaaa tctttcatct ggattttgta gagatattag agaagcagta
    27901 tttataaaat cacaagattt atcaataaat gattttaata atataggtac agcatcaatg
    27961 attactagga gtactagtga tattacttta ataggtcagt ctgtgtttat gtttattcaa
    28021 ttagtattac cagcaccaat tataactgtt tctggtctat tcttagcata ttctatagat
    28081 aaagcaatga caattattat tgttgtagta atgtttttat tcatgctttc agcattttta
    28141 gttggtaaaa agcttataaa gttgtttaaa atgatgcaaa taaaaatgga caatatgaat
    28201 agggtgttac gtgaggtagt aactggagta agagttataa gagcttttaa taggagtcat
    28261 tttgaaaaaa agagatttga tagaactgca atagattatt ctgaaacagc aatttcaata
    28321 aataaaatat ttgcagtact tatgcctatt gtaatgttaa taatgaattt aggtatagta
    28381 agtattattt ggtttggtgg gatgagagtc agtaatggaa atatggaaat aggtcatata
    28441 atggcacttg ttgaatattg tatcttaatt ttattctatt taatcatggg agtaatggta
    28501 tttatgtaca tacctagagc aggtgcatgt gcagatagag taaatcagat attagatata
    28561 gaacctgaaa ttgtagatgg aaatggtcat aaagatactg tctcagagag aggacattta
    28621 gtatttaaaa atgtaacctt ttcatatgca caatcagaag aacctgtgtt aaataatata
    28681 acatttgaag caaaatctgg agaagtaact gcaattatag gaagcactgg ctcaggcaaa
    28741 tctactattg caaatataat accacgtttt tttgaaattc aatctggaga aatatctata
    28801 aatggtcagg atattaagaa gattcctcaa aaagaactta gagataagat tggatttgta
    28861 ccacaaaaag cttttttatt tagtggaaca atagaagaaa atataaggta tggaaaagaa
    28921 gatgcaagta ttgaagaagt aaagcatgct gcaagtatag ctcaagctga tgaatttatt
    28981 tctgatatgg aagacaagta tgactcattt gtagcacaag gtggaaataa tttgtcaggt
    29041 ggtcaaaaac agagaatatc cattgcaaga gcgttagtta gaaagcctga agtatatgtc
    29101 tttgatgata gtttttctgc attagatttt aaaacagata aaagacttag aaaagcgttg
    29161 aaaaatgaaa tcaaagattc atcagctata attatagcac aacgtataag tacaatcatg
    29221 gatgctaatc agattatagt tttaaatgat ggaaaaattg taggtattgg aaaacataaa
    29281 gatttacttg aaaattgtga agtttataag caaattgcgg attcacaact tagtaaagaa
    29341 gaacttgcat aaaatgaaga attaatctga tatttggagg tgtatgatat gagtgaaaaa
    29401 agaatagatg aacaagatat agaagtactt agttttgaag atggcatgtc agcagaaaaa
    29461 gctaaggatg caaaaaaaac tactaagaga ttattaaaat atatggcaaa acaaaagttt
    29521 aaaatattaa ttatttttat ttcagtattg ataagttcag cgcttacagt tttagcacct
    29581 atggttatgg gaaaagctat tgaccaacta tttaatggaa taaaaactgc agtccaaaca
    29641 ggtacaaaat tcagtgtgaa ttttagtact atggggggga tagtatcaat acttctaggg
    29701 ttgtacttaa ttagtgcagt atttatatat attcaacagt atattatgtc aggagttgca
    29761 caaaatctag ttcttagcat gagaaaagat ttaagtgata aactcaataa acttcctctt
    29821 aagtactatg attctcataa aaagggggaa acacttagta tagtaacaaa tgatttagaa
    29881 aaagtagcag atagtttaca agaaggatta atgcaactta ttacagcagt agttacagta
    29941 gttggttcta ttgtaatgat gataagtata agtgtaccac ttacaatagt atcagctata
    30001 acattacttg tttcattggg tattactgtt gtaattgcaa gaaaatcaca agaaagattc
    30061 tctgaaaatc aaaaggcact aggagagtta aatagcaata tagaagagat atttactggt
    30121 caaatagtgg ttaaagcatt tagtaaagaa aaggatacta taagaaactt taaaatatta
    30181 aatcaaaatc tttataatgc aagtagaaaa gcacagtttt cgtcatatgc aatatctcca
    30241 attattagat ttataaacct aattggctat gttattatag cagttgtagg tgggatattt
    30301 gcatcaactg gagcaatgac tcttggtagt atacaagcgt ttattcaata tgtaaatcaa
    30361 gcatcagagc ctactacaga aatatcttat attgtaaata tgcttcaagc agcaatagca
    30421 tctgcagaga gagtttttac agttatggac gaggtggaag agataaagga tgaagaatct
    30481 tcaaaggtta ttagcatgcc taaaggtaaa gtacaatttg agcatgttaa atttggatat
    30541 agtgatgatt ttattcttat gaaagatatt aatatcaatc taaatgcagg agataagatt
    30601 gcaattgttg gacctacagg agctggaaag acaactcttg taaatttact tatgagattt
    30661 tatgagatac aaggtggaag aattactatt gatggtgtta atataaaaga tttaaagcgt
    30721 ggagaactta gaactatgtt tggtatggtt cttcaagata catggctgtt taatggaagt
    30781 ataaaagaaa atattgcata tagtaagtca gatgccacaa tggatgaaat tgtaagagca
    30841 gcaaaatcag caagagtaga ccattttata aggactttac cacaagggta tgacactata
    30901 ttgactgagg atgcttctaa tctatctcaa ggtcaaaagc aacttctaac aattgccagg
    30961 gcaatacttt cagacccatc agtattgatt cttgatgaag caacatctag tgtagatact
    31021 agaacagagg tagaaattca aaaggcaatg aataacttga tgaagggaag aactagtttt
    31081 gttattgcac atagattatc aactattcga gatgctgatt taatattagt aatgaaagaa
    31141 ggaactatta ttgaacaagg aagccataag gaacttattg aaaagaaagg attctatgaa
    31201 gaactttata acagtcaatt tacaagtgaa tatgatgaag atgttgtatg attatcaagt
    31261 tttaggcaac taaatgattg ataaatatta agttaaatat agtaacatgt aaggttatat
    31321 ggaataagag tatttctcta tttcatataa ccttttttat ttatcaaaat aagataaaat
    31381 ttcctttcta aattagattg aagaatgtat ttatcaatat aaaagtaaaa taatcattat
    31441 tattagtgaa gtaaatattg tttgccacat atatgccata aagatgcact tattttacgg
    31501 agatttaaca aagtagtgta aagtatactc taaatataaa ataaatcaaa tagagttaaa
    31561 taaattgaga ggtgatataa atagtgaata aaaaattgaa ctatagattt gaaatgaagc
    31621 acaaaataac tgaagcagat gtgttagctt taaaatcaag actctatcaa ataatgaaga
    31681 aagatgaaaa tgcatctaaa gatggaaaat atcttattag gagtttatat tttgatactc
    31741 ctgaagataa agcacttttg gataaattaa atggcgttgc aataagagaa aagtttagga
    31801 taagatttta taataatgac tgctcttata taaggctaga gaaaaaaatc aaacattaca
    31861 acatgacatc aaagctatca actaatttaa ccaaaaaaga agtaataaat atactaaata
    31921 atgatattga atttctaaaa gagtcgacaa attgtttatt aagagaattt tatttgaaat
    31981 taaaatgtga aagattagag gcaaaaagca tagttgatta caatagagaa gcttttattt
    32041 atccagttgg aaatgtaaga gttacaatcg atagtgatat taaaacttct gtaaattcgg
    32101 tagatttatt taataaagat ttgccaactg tttcggtaat tgatgagaat atgactgtat
    32161 tggaggttaa atatgatgaa tttataccag attttataaa ggatttaatt caaattaata
    32221 agactacctc tacagcagtt tcaaagtatg cttcaagtag attatatatt taagaataaa
    32281 ggctatgatt tatataaaga tatacataaa taaggttaaa aaacaaataa gttaaaaata
    32341 aataaaataa aaaataaata agttaaaaat aggaaggatg attttaatta tgacatttaa
    32401 tgatattttt aaatcaagtt ttatagaaaa cgtatcaggt ttttcatttg tggacagtgc
    32461 tttagcacta ggttcagctt ttttagtagg tttgtttata tatatggtct ataaaaagac
    32521 ttatatggga attatgtatt ctcgaccatt caatgtgtcg cttgtagcac ttacaatgct
    32581 tacaactttt gtaatattgg cagttacttc aaatgtagtt ttatccttag gtatggttgg
    32641 tgctttatct atagtaagat ttagaacagc aattaaagac ccaatggatt tagtatttct
    32701 attttggtca ttgggttcag gtatagtttt aggagcaggt ttgattccct tagctgtcat
    32761 gggttcaata attatgggat tgattctaat attcttttca aataaaacaa tatctgaaac
    32821 tccatatata ttgatggtaa attgtaataa tgaagattca gaagatatag caactgataa
    32881 aattaaaaaa gtatttacta aatatcaaat aaaatcaaaa agtgttacac cagaaaaagg
    32941 aatagaatta gtgtttgaag taagaatgaa agatggagaa acaagtctta taaatgactt
    33001 gagtcaagta gatggagtta ctaatgcagt tcttgtaagt tataatggtg actatgtagc
    33061 atagagagga gtaatctttt atgaaagata aaaaatttac ccttcttatc tcgattatga
    33121 ttatattttt atgtgctgta gttggagttt atagtacatc tagcaacaaa agtgttgatt
    33181 tatatagtga tgtatatatt gaaaaatatt ttaacagaga caaggttatg gaagttaata
    33241 tagagataga tgaaagtgac ttgaaggata tgaatgaaaa tgctataaaa gaagaattta
    33301 aggttgcaaa agtaactgta gatggagata catatggaaa cgtaggtata agaactaaag
    33361 gaaattcaag tcttatatct gtagcaaata gtgatagtga tagatacagc tataagatta
    33421 attttgataa gtataatact agtcaaagta tggaagggct tactcaatta aatcttaata
    33481 actgttactc tgacccatct tatatgagag agtttttaac atatagtatt tgcgaggaaa
    33541 tgggattagc gactccagaa tttgcatatg ctaaagtctc tataaatggc gaatatcatg
    33601 gtttgtattt ggcagtagaa ggattaaaag agtcttatct tgaaaataat tttggtaatg
    33661 taactggaga cttatataag tcagatgaag gaagctcgtt gcaatataaa ggagatgacc
    33721 cagaaagtta ctcaaactta atcgttgaaa gtgataaaaa gacagctgat tggtctaaaa
    33781 tcacaaaact attaaaatct ttggatacag gtgaagatat tgaaaaatat cttgatgtag
    33841 attctgtcct taaaaatata gcaataaata cagctttatt aaaccttgat agctatcaag
    33901 ggagttttgc ccataactat tatttatatg agcaagatgg agtattttct atgttaccat
    33961 gggattttaa tatgtcattt ggtggattta gtggttttgg tggaggtagt caatctatag
    34021 caattgatga acctacgaca ggtaatttag aagacagacc tctcatatcc tcgttattaa
    34081 aaaatgagac atacaaaaca aaataccata aatatctgga agagatagta acaaaatacc
    34141 tagattcaga ctatttagag aatatgacaa caaaattgca tgacatgata gcatcatatg
    34201 taaaagaaga cccaacagca ttttatactt atgaagaatt tgaaaaaaat ataacatctt
    34261 caattgaaga ttctagtgat aataagggat ttggtaataa agggtttgac aacaataact
    34321 ctaataacag tgattctaat aataattcta atagtgaaaa taagcgctct ggaaatcaaa
    34381 gtgatgaaaa agaagttaat gctgaattaa catcaagcgt agtcaaagct aatacagata
    34441 atgaaactaa aaataaaact acaaatgata gtgaaagtaa gaataataca gataaagata
    34501 aaagtggaaa tgataataat caaaagctag aaggtcctat gggtaaagga ggtaagtcaa
    34561 taccaggggt tttggaagtt gcagaagata tgagtaaaac tataaaatct caattaagtg
    34621 gagaaacttc ttcgacaaag caaaactctg gtgatgaaag ttcaagtgga attaaaggta
    34681 gtgaaaagtt tgatgaggat atgagtggta tgccagaacc acctgaggga atggatggta
    34741 aaatgccacc aggaatgggt aatatggata agggagatat gaatggtaaa aatggcaata
    34801 tgaatatgga tagaaatcaa gataatccaa tagaagttgg aggttttggt aatagaggag
    34861 gaggctctgt gagtaaaaca acaacatact tcaaattaat tttaggtgga gcttcaatga
    34921 taataatgtc gattatgtta gtaggtgtat caagggtaaa gagaagaaga tttataaagt
    34981 caaaataaga ttaaaattga atataaataa ttttaatact gttaagtcta aatatttact
    35041 tagcagtatt ttttagaaaa taatgtgttt gattaataat ataaggtaat aaattgataa
    35101 aaattaaata cagcatatta ataatacata ataaagtaat aaaaaactgt ttaagtataa
    35161 taacttatat ttagacagtt tttttcattt ataaataata taaatgaaat tgaaaacatt
    35221 tgaaggatag aaattggttg gataaatgta taaattactt caccaaaaaa taatgacaaa
    35281 aactgtaact aagttgtaat aaaatggtag ttatttagca taaaataatg tatcatagaa
    35341 gtatattata aaattaataa aataagattt ttgtaagaaa actgctggct tttgatattt
    35401 agagtgagta gttaaattat gtttaggagg agtagaattt gttgcaaaaa gtattatgga
    35461 aatttttaaa gctagtagtc ggtttattta tatgttcagt tggtatagta ttgacgataa
    35521 attgtaacct tggattatct ccctgggatg tatttcatca aggactttca aatcatatag
    35581 gaataactat aggaactgct tctataattg taggctctat agtagttatt gctgatgtcg
    35641 ttcttggaga aaatgtagga tggggaactg tttttaacat gctactaatt ggatttttta
    35701 tggatttact tttatatagt aatttaattc cagaggctga taatttattt gtaggaataa
    35761 ttatgttgat attaggattg gttcttttgt ctgttggaat ggtattttat atgggaagtg
    35821 gtttaggaag tggacctaga gatggtttga tggttgcaat tcaaaagaaa acaggaaaat
    35881 cacttaaatt aataagaggt actatagaag ttggagcatt aatagtagga tttttattag
    35941 gtggaaaagt tggtatagga actataataa gtgcatttgg attggggtat ttcacacaaa
    36001 tggtatttgg cttatttaaa ttagattgta gtaagattaa acatagattt atagtagatg
    36061 atataaaatt tatcaaagtg tatatgagtg gtgagaaaaa agctgctgtt caaaatatag
    36121 ttaagtaatt taagaataaa ttcaacgaaa tgttaagtat tttaaattaa cattttgttt
    36181 agatagataa attgaatatt aaaagaatat taaaatatga ggattgctag aacactataa
    36241 aatatagatt tctagcaatt ttttatttgt tcaatatttt tttagtacta gaagtcttat
    36301 tctctattta atgaatgaat taatttataa ttagagatac taaactttat agatattaat
    36361 ataaacacct ttaaactgtg tattttccaa agcctttaaa atgcttgtat ttattctaaa
    36421 taaaaaaata aaaaaatgtc aaaaaaactc ttgtattata tccaaatttt atatataatt
    36481 acctatgttg tttagaaatt ttaaactcaa tgtttagtaa acaaaatgtt tagtaaaata
    36541 aaacagaaac taggaggtgc aacatggata taggtgaaaa gataaaaaga ttaagaacag
    36601 aaaaacaact gacccaggaa gagttagcta atagatgtga attgtcaaag ggatttattt
    36661 ctcaattaga aaataactta acatcaccat ccatagctac ccttatagat atacttgaaa
    36721 tactaggtac aaatttaagg gaatttttta atgaaataga tgatgaaaga atcagtttta
    36781 caaaagaaga tatgtttgaa actgaagatg aagatttaaa atataaatta aagtggttaa
    36841 ttccaaattc acaaaaaaat gaaatggagc caataataat aacattgtat ccaggaggac
    36901 agtataaaga agaaaagcca catgaaggtg aagaatttgg atatgtatta gcaggttcaa
    36961 tatatgtcca tataggcgaa aagaaaaata aagtaaaaaa aggagagagt ttttacttta
    37021 gaccgaaagc aaatcactat atatcaaacg agggcaaaac tacagcaaaa gtgatttggg
    37081 taagtacgcc accatcattt tagaatagag gtgtttaata attggtagaa aatataatag
    37141 aattaaggga tatatctaaa cattatgaag aacttactgt tttagataat ttcaatttag
    37201 atataaagaa aaatgagttt ctaacattgc ttggaccaag tggatgtgga aaaactacaa
    37261 cattaaaaat aatagcagga tttgagtatg ctgatgatgg taaagtttta tttgaaggta
    37321 aggagataaa taatttgcca ccatatgaaa gacaagtaaa cacagtattt caaaaatatg
    37381 ctttatttcc acacatggat gtttatgaaa atgtagcttt tggattaaaa attaaaaaga
    37441 ctccaaaaga tgtaatagat gctaaagtaa aagaggtttt gaaacttgtt gcactagaag
    37501 gttttgaaag acgacaagtt gaatcattga gtggaggaca acaacaaaga attgcaatag
    37561 caagagctct agtcaatgaa ccaaaggtat tattactaga tgaaccactt ggagcacttg
    37621 atttaaagtt aagacaagag atgcaaatag aattaaagag aatccaaaaa aaattaggaa
    37681 taacatttgt ttttgtaact catgaccaag aagaggctct tactatgtca gatactattg
    37741 ttgtaatgta taagggcaaa attcaacaaa tgggaacacc acaagatata tacaatgaac
    37801 ctaaaaattc atttgtagct aagtttatag gtgaaagtaa catatttgat ggaattatga
    37861 tagaagatta taaagttaat ttctgtaata gagattttga gtgtgttgat aaaggattcg
    37921 aaaaaaacga aaatatagaa gtagtaataa gaccagaaga tataaaaatg gtaaagccag
    37981 aagaaggaat gttaaagggg aaagttacat ctacagtgtt taaaggagtg cattacgaga
    38041 tagaattgaa tgaaaatgga agattgtgga tacttcataa tactaaaaat gctgaggttg
    38101 ggactgaact aggcatggat atatatccag aagatatcca tataatgaga aaagagaata
    38161 actaagatga aaagaaaatc ttttttagca tatccatatg tagtttggag tgcgatattt
    38221 gtaataattc ctttaatatt agtagtattt tttagtttta caaaagaatc aggtggaggt
    38281 tatgccttta cacttgagaa ttataaagaa gtaattgacc ctatatatat gaaagttttt
    38341 gggcgttcta ttcttttagc aggaggagct acattaatat gccttatagt tggttatcca
    38401 gtggcatata taatttcaaa ggctcgtgta agtagaagag gttcattaat attattgttt
    38461 attcttccta tgtggatgaa ctttctttta agaacatatg catgggttgc tatattagga
    38521 aaaaatggac ttttaaatac atttttaggt tggtttggaa tacaaccatt agcaattttg
    38581 tatactaatt ttgctatact tttaggtatg gtgtataact ttttaccatt tatggttctt
    38641 ccaatatata cagcactatc aaagatggat aatgatttga taaacgctgc tcatgacttg
    38701 ggtgcaaata atatgacagt ttttagaaaa attatatttc ctttgagttt gccaggtgta
    38761 atgtctggaa taactatggt atttatgcca gcagtcacta cttttgcaat atcaagactg
    38821 ttaggtggag gtaaaataat gctggttgga gatttgattg aacaacagtt tacagttgtt
    38881 ggagattgga actttggttc agctatatcg atatttatga tgatagtaat attaatatct
    38941 atgtctataa tgtcaaaatt tggagatgaa tctgacaaag aaggcggtgg gttactattt
    39001 taaaattaaa gaaaataact tctaatatat atttagcatt agtattctta tttttgtatg
    39061 cacccatatt tgctttggta ctattttctt tcaatgattc aaaatcaatg gcaagatgga
    39121 atggatttac atggaagtgg tatggtcaat tacttcaaaa tgagagtata atgagtgctt
    39181 tatattatac tattgtaata gctatattag cgtctgtaat atctacaata gtaggaacta
    39241 taagtgctat aggaatacat aagatgagag gtaagagtaa gaaattaata ctaaatgtaa
    39301 actatcttcc tattttaaat acagagatag ttacagcagt agctcttatg agtctgtttg
    39361 tatttgtaaa aatggaattt ggatttacaa caatgctatt ggcacatata atgttttgtt
    39421 tgccatacgt tatactttca gttttgccaa aaatgaagca attacctgat aatatagaag
    39481 atgcagctat ggacttggga gcaacaccta tatacgctct tagaaaagta attttaccac
    39541 aaataaaacc aggtatagtt tctggatttt taattgcatt tactatgtca atagatgact
    39601 ttataataag tttctttaat gctggaaatg gtgtaagcaa tctttctata gaaatctatg
    39661 gaatggctag aagaggtata aaacctgaaa taaatgcatt atcaacaata atgtttgcag
    39721 tggttctagg tttactttta ctagctaata aaaaagaatc tatagtaagg gggattaaat
    39781 aatgattaag tttaaaaaag caatatccct tatagctgta ggtatgatga cagttacttt
    39841 atttacagga tgtaataaag caactgattc tacaaaagtt ttaaatgtat ataatgttgg
    39901 agattatata gatgagtctc ttatagataa gtttgaaaaa gaaacaggaa tagatgttca
    39961 atattcaact tatgatacta atgaaatgat gtatcaaaaa gtaaagagtg gtagtacaca
    40021 ctatgattta gtatttcctt cagactatat ggtagagaaa atgaaaaatg aaggtctttt
    40081 ggaaaaatta gattttaaga atatacctaa tatgaaatat ttagataaga gttttttaaa
    40141 tcctgtatac gacaaaacta atgaatatag tgttgcatat atgtggggaa ctcttggaat
    40201 attgtacaat aagaaagaag ttaaagaccc tatggatagc tggaatatac tttggaatcc
    40261 aaagtataag ggaaatataa tgatgtttga ctctgttaga gatactatag ggataactct
    40321 taaaaaatta ggatatagca tgaatagtgt aaatccaaaa gaaataaatg aagctaaaaa
    40381 tcttttaatg aaacaaaagg atttagtatt ggcttatgta aatgatgaag gtaaggatag
    40441 attgcttggt ggagaagtgg caatgggtat gctgtattct ggagatgcag ttacactaat
    40501 ggagcaaaat cctgaccttg attatgctat acctaaagag ggaactaata aatgggtaga
    40561 tgctatgtgt gtacctacaa cagcacaaaa taagaaagaa gctgagctgt ttataaactt
    40621 tttattagac ccagagaatg caaaagtaaa tgctgagtac attgggtatt caacacctaa
    40681 tacaggagca ctaaaattat tagacccaga aataactcag aatcctgttg catatccatc
    40741 taaagaagta cttgataaat gtgaaacttt tgaagatatt ggtgaaaata taaaattata
    40801 tgacaaggct tggatagagt taaaatcaaa atagggtctt gttgttttaa gtaaatttac
    40861 ttaataaata cttaataaaa ctgttctttt ttagaaaatt tattttctaa ttattgaaca
    40921 gttttatttt ttgtttaagt tatattcata tgcaatttaa atagtttatt aataaatgaa
    40981 aacttatttg tataatgact ttaataataa ttagaaaaaa tgggcaactt aatagaataa
    41041 ttggaggtgt aaatttataa aaaatgaata ggataaataa aaaaatagat ttattaatat
    41101 tggctttgct cgtttttata tttttaattg ttggaatagt aatactatcg attaggacag
    41161 aaaataaatt gaatgtgttc ataatgttag caataatttt ttttataata atgttaactt
    41221 atttatcaaa ttcagtagtg gggttgataa caagttcaat aattatattt atgtatacat
    41281 cctatatttt atataacaat attactcaca atatggatgt tgaatttata tcatatatgt
    41341 ggataatagc aacaccagta tcttcaatta taatgggtaa tctgaacaaa agcataaatg
    41401 aattgcaaaa
//
LOCUS       DACULQ010000013        40828 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.013, whole genome shotgun sequence.
ACCESSION   DACULQ010000013 DACULQ010000000
VERSION     DACULQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.013"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(277..1086)
                     /locus_tag="JA154_02990"
     CDS             complement(277..1086)
                     /locus_tag="JA154_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7994823.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQKFLQFLEE"
     gene            complement(1170..1703)
                     /locus_tag="JA154_02995"
     CDS             complement(1170..1703)
                     /locus_tag="JA154_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAT7994824.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(1853..3202)
                     /locus_tag="JA154_03000"
     CDS             complement(1853..3202)
                     /locus_tag="JA154_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HAT7994825.1"
                     /translation="MELKNFKDILFKKALSEGFEEYEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(3214..4596)
                     /locus_tag="JA154_03005"
     CDS             complement(3214..4596)
                     /locus_tag="JA154_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HAT7994826.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            4840..6054
                     /locus_tag="JA154_03010"
     CDS             4840..6054
                     /locus_tag="JA154_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAT7994827.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFNFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            6252..8144
                     /locus_tag="JA154_03015"
     CDS             6252..8144
                     /locus_tag="JA154_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAT7994828.1"
                     /translation="MKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLKEGIPLAE
                     KSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEPFGIIGFN
                     NVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAHIVKRLNC
                     KGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVDKNFKITL
                     FNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTKIITNRVP
                     IIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEKTKDCIEL
                     AKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSLIESEFFG
                     YEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQVMRIGGDK
                     VIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDIIKLSEFFI
                     HKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSILLEKVSNE
                     YTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRSTLWRKLK
                     EIEDDV"
     gene            8415..9389
                     /locus_tag="JA154_03020"
     CDS             8415..9389
                     /locus_tag="JA154_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HAT7994829.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIIALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            9410..9685
                     /locus_tag="JA154_03025"
     CDS             9410..9685
                     /locus_tag="JA154_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate hydrolase"
                     /protein_id="HAT7994830.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            9698..10861
                     /locus_tag="JA154_03030"
     CDS             9698..10861
                     /locus_tag="JA154_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAT7994831.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKTGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            11198..13478
                     /locus_tag="JA154_03035"
                     /pseudo
     CDS             11198..13478
                     /locus_tag="JA154_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(13573..14913)
                     /locus_tag="JA154_03040"
     CDS             complement(13573..14913)
                     /locus_tag="JA154_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT7994832.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEEILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(14925..15707)
                     /gene="fecE"
                     /locus_tag="JA154_03045"
     CDS             complement(14925..15707)
                     /gene="fecE"
                     /locus_tag="JA154_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089388.1"
                     /note="Part of the FecBCDE citrate-dependent iron (III)
                     transport system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HAT7994833.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(15722..16744)
                     /locus_tag="JA154_03050"
     CDS             complement(15722..16744)
                     /locus_tag="JA154_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAT7994834.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(16750..17763)
                     /locus_tag="JA154_03055"
     CDS             complement(16750..17763)
                     /locus_tag="JA154_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome ABC transporter permease"
                     /protein_id="HAT7994835.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTKFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(17891..18829)
                     /locus_tag="JA154_03060"
     CDS             complement(17891..18829)
                     /locus_tag="JA154_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT7994836.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEDKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            19099..19563
                     /locus_tag="JA154_03065"
     CDS             19099..19563
                     /locus_tag="JA154_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994837.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILDGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(19675..19992)
                     /locus_tag="JA154_03070"
     CDS             complement(19675..19992)
                     /locus_tag="JA154_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAT7994838.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(20005..20736)
                     /locus_tag="JA154_03075"
     CDS             complement(20005..20736)
                     /locus_tag="JA154_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAT7994839.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(20762..22072)
                     /locus_tag="JA154_03080"
     CDS             complement(20762..22072)
                     /locus_tag="JA154_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089395.1"
                     /note="catalyzes the fromation of N-acetyl-D-glucosamine
                     and N-acetyl-D-glucosamine-6-phosphate from
                     diacetylchitobiose-6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAT7994840.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(22169..23482)
                     /locus_tag="JA154_03085"
     CDS             complement(22169..23482)
                     /locus_tag="JA154_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT7994841.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(23504..23815)
                     /locus_tag="JA154_03090"
     CDS             complement(23504..23815)
                     /locus_tag="JA154_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT7994842.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(24015..24719)
                     /locus_tag="JA154_03095"
     CDS             complement(24015..24719)
                     /locus_tag="JA154_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7994843.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCEYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(24810..26171)
                     /locus_tag="JA154_03100"
     CDS             complement(24810..26171)
                     /locus_tag="JA154_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT7994844.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            26670..27824
                     /locus_tag="JA154_03105"
     CDS             26670..27824
                     /locus_tag="JA154_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT7994845.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(27906..29624)
                     /locus_tag="JA154_03110"
     CDS             complement(27906..29624)
                     /locus_tag="JA154_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HAT7994846.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLFTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(29629..30618)
                     /locus_tag="JA154_03115"
     CDS             complement(29629..30618)
                     /locus_tag="JA154_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HAT7994847.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(30714..31763)
                     /locus_tag="JA154_03120"
     CDS             complement(30714..31763)
                     /locus_tag="JA154_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT7994848.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(32040..32708)
                     /locus_tag="JA154_03125"
     CDS             complement(32040..32708)
                     /locus_tag="JA154_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAT7994849.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(32714..34087)
                     /locus_tag="JA154_03130"
     CDS             complement(32714..34087)
                     /locus_tag="JA154_03130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089471.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAT7994850.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(34084..35862)
                     /locus_tag="JA154_03135"
     CDS             complement(34084..35862)
                     /locus_tag="JA154_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase alpha chain"
                     /protein_id="HAT7994851.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(35979..36296)
                     /locus_tag="JA154_03140"
     CDS             complement(35979..36296)
                     /locus_tag="JA154_03140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089473.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the F subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAT7994852.1"
                     /translation="MYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVEDEY
                     AIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGADIL
                     FKE"
     gene            complement(36289..37266)
                     /locus_tag="JA154_03145"
     CDS             complement(36289..37266)
                     /locus_tag="JA154_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HAT7994853.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(37281..37844)
                     /locus_tag="JA154_03150"
     CDS             complement(37281..37844)
                     /locus_tag="JA154_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994854.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(37896..38387)
                     /locus_tag="JA154_03155"
     CDS             complement(37896..38387)
                     /locus_tag="JA154_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAT7994855.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(38390..40315)
                     /locus_tag="JA154_03160"
     CDS             complement(38390..40315)
                     /locus_tag="JA154_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAT7994856.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVIFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(40317..40628)
                     /locus_tag="JA154_03165"
     CDS             complement(40317..40628)
                     /locus_tag="JA154_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994857.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
BASE COUNT        13777 a         7037 c         5213 g        14801 t
ORIGIN      
        1 tatctttttt cacatataat ttaatttcta aaacggttag tttgacatta ttggaatata
       61 aacaaaaatt taatataaat tcgttaatat attaatacat ttttaagaat gacaaggaat
      121 aaattataac aaattttagg aatatataat aatagggtct cagaacttag ttacaagttc
      181 taaaacccta tttgtttagt gtaaaaaaca tcataaaatt tagcttaaat cttttcacat
      241 aatattactc tttttctaag tgataataaa atttatctat tcctctaaaa actgtaaaaa
      301 tttctgatta aatagtttaa gctcatcata gaatatagca tgaccactat actcaaaagt
      361 atataaaatt gaatcttcta ttttttcatt cataaaaact gcaaattcat acggacatat
      421 ttcatctaat ttcccatgaa atataccagt aggcactctt acacatttta aatcattaaa
      481 taattcttca tctcttaaag atacagcagt tccaatagtg ccaatacctg atgcactaaa
      541 accaatatca ttaaaccatc ttctaaagct ttctggtgga tttgatgcaa atactttttc
      601 gccaaaatcg gttaccattt ctggtctatc agtacatgct tgtgctatca atttatttac
      661 atcttcacgt gtcataccat atggaaattc tggaggtctt tgtacaaatg atggtgcagc
      721 agctgcagct aataccaatt ttgacactcc atatccatta aataaactca tatacctaag
      781 tacgattgct cctcccattg aaaaaccaac aagggtaaag tcttttaatc caatagcatg
      841 taccacctta taaatatcgt ctgctaattg gctataagta tatcctcctg atgttgcatc
      901 agatttacca aaacctctta aatcaattga aactgttcta tatccgagtt ttggcaatat
      961 atttgtttga tattcaaata tcttatgtcc taagggccaa ccatgtataa aaagtacagt
     1021 ttttttagca ctcggattca aatcatatac tgctatgttt atatcattca catatacata
     1081 atacatatca acagcaactc ccttcaagaa aatgtatgat ggttatttta tatatatgaa
     1141 taaaatgatg aataactaaa ttttcaaatt tatttacaat acttctctat tgccttttca
     1201 aataaactta catgaagttt ttcatctagg attattctat ttaatatagc ttttatataa
     1261 gggtcttcaa taagacttat atctttataa taattattaa tagccacatg ctccaaatgc
     1321 aaatctagtt ttaatctgtt acaaatagaa tttccataac atacaaaacc accattccaa
     1381 tatgcaccac aagaacttgt agaacctcta tatacaggat ttccacccaa taatcttata
     1441 gtctttgcta atatctccat atgtaacatt tctgttatag atatatttat ccacatttca
     1501 cttaactctc tatgcgtttc atctaaatca aaattatggt atagatattg atttacactc
     1561 gtaaattcac ttgatactcc ggcatagtca tccatcaata attctgcata atacttattt
     1621 ggacctaaaa cttttatctc tggatatggc tcatcacttg aatatccatt tcgcttgtgc
     1681 atgtcataat gtgcatcact cataatatct cctcctttaa atttaataca tgatattaat
     1741 tgtatgcatc acgacttttt catataactt agataataaa atctacttaa ataataaaaa
     1801 gccatattaa ttaaaattaa catgaccttt tatccacaaa attacttttt aattattcat
     1861 cttttccagc tactgatagc tctttaataa caactgaagg tgaacctata ctactcattg
     1921 gaaattctaa atcttcccct atagcttcaa tatctttcaa caggtcaaaa tagtttcctg
     1981 ctactgtaat ttgctctact ggaaatactt tttttccttc tttgatataa aagcctttgg
     2041 cagctaaaga aaagtctcct gttactgaat ttgcacctga atgaagtcct gcaaagctag
     2101 taaccatcaa tccttcatct atatccttta taatttcatc taaagattta tctcctttct
     2161 ctatataaaa attagttgga tatatgctta taggtgatga atatgaagat tttattccat
     2221 ttcctgtagt ctttacacct gccttattag ccgtttttaa gttatgaagt aaagttataa
     2281 gctttccatt taaaacaact tcttttttaa atgtagctac tccttcatca tcaaaaggag
     2341 tagatgccat accattttct aaaagtgggt catcaactat tgtaactata ggagatgcta
     2401 tcatgtctcc ttctctattt ttcaataaag ataatccttt ttgagctgaa tctgcattaa
     2461 atatcccaga aaatgtgcta agcaaagata ccatagcttc attaagcaat atagttttat
     2521 atttacctga tggaatactc ttaccaccta ctttagataa agcattatca acaccaagtt
     2581 ttgctatctc acaaggtttt atttcttcaa tagaatttgc tatattatat cctattccat
     2641 catatttttg tccattgtct tctatgatag gaactacaaa ccctataagc atattagttt
     2701 tattgcttaa attaagacct ttagtattag atataccatt acttgataca gaataagata
     2761 tagtacattt agataaattt accaccttat ctgaataagc tttagtctct ctttccattt
     2821 ctaaagctaa atcaatcaac ttatctgcct ctaaattttc tagctcttta gaataagtct
     2881 ttacatcatt atagtgctta tctccttcat atataaattg tacatcttca ttttcaatag
     2941 tgtttactcc atcttttaca ttttttatca acatatcaac agctttatca tctaaaattt
     3001 ctgtataaga ataacctatc tttccattta ccttacctct aaaagataaa ccaaaagatt
     3061 tatcaagatt gtatttctca acttctcctt catatatatt tatacttaaa ttttctcctg
     3121 tataataata tatttcatac tcttcaaaac cttcacttag agccttttta aataatatat
     3181 ctttaaaatt tttaagttcc acaattatcc ctcctatctt ccacctacag taatttcttt
     3241 tacccttatc ataggttggc ctacattagt tggtatactt cctgatgatg agccacacat
     3301 cccctgagct tgttttacgt tattcccaac catatctata ttcataagta tatcgctacc
     3361 ttttccaatt aaactagcac ctctaactgg ctcttgtatt tctccatttt ttattaagta
     3421 accttctgaa actgcaaaat taaactctcc tgttacagga tttacagaac caccacccat
     3481 ttttttagca taaagtccat ctggtattga ctttataata tcttctggtc tatcttctcc
     3541 agcagcaata taagtgttag tcatccttga agttggtgca aatttgtagt tctgtctacg
     3601 agaacttcct gtaggattca ttcccattcg tctaccatta agtttatcta tcatataaga
     3661 cttcaaaatt ccattttcaa tcagtatatt cttttgagtt ggatttcctt catcatctat
     3721 attcatagaa ccccagtagt ttggtattgt accatcatct atagctgtaa tctttgtgga
     3781 agcaatctgt tgacctaatt tatctgcaaa tacagagttc ccctttgcaa ctgatgtagc
     3841 ttccaaagaa tgtccacaag cttcatgaaa tattactcca ccaaatccat tatctattgc
     3901 taccatcata ttacctgcag gacaattttt agcatgcaac atagtatgcg ctactctagc
     3961 tgattctttt gcataatatt ctgggtctat ttcttcaaat atttcaaacc ctttacaacc
     4021 cccaggacct tcaaatccag tttgattttc attaccctta gatgcaattg aacttatccc
     4081 tagtctggtt cttattcttc tatcctcaac atatatacct tcagtgtttg caattaatat
     4141 tttttggtct ttatccaaat agctaacact tacttgagat atatcattac taaattcttt
     4201 tgcactctta tatgcaacct tcataacact tactttatct ttatttccta ttgaatttgg
     4261 ataaattttt atattatgta tgttatttat tttaatactg tcatttaata ttacactttt
     4321 tccatcttcc aatttaccta aagcaacagc agctttgtat gctacatcca acagactatt
     4381 taatgaattg tcacttgtat aagcatatat acttttcaat cctttaaata ttcttattcc
     4441 tataccataa tttcttccac ctattgcatt ttctacttta ttatctatta aactaataga
     4501 attatttata gtgtcttctt caaaaatctc agcaaaatct cctccagtaa ttagacattt
     4561 ttctaatacc ttagaagcta tattttttga aagcataaat ttacctccta ctttaaattt
     4621 ttaattaact tttattcaat ctttttactt gttaacaaac attatatact atattactta
     4681 aaataaggta attatgaata tattatacat atgttttata ctaaaatgtt atcacatttt
     4741 atctatacat acccatataa ctagataatt aaaatgtata atattatttg ctatagaaaa
     4801 taatacactc atacataata aattttagga gtgaagtata tgaatcaaga atatgtaaaa
     4861 ggaaatccta tttttaccaa tattaataaa gttccaagac aatttccata ccttactgat
     4921 gatattgata ctgatgttat aattgttggt ggtggcgtaa ctggatgtat ttgtgcctac
     4981 tatctagcta aaaacaatat aaagtctgtc attcttgaaa aaggaaggat tgcacatgga
     5041 agcactagcg tgacaacttc tctattacag tatgaattag atgacaattt gatagattta
     5101 acagaggtta tgactctaaa agatgctcta aaagcatata acctttgtac ttcagcactt
     5161 gaagaattag atacatttat agagctatat ggaaataagt gtgattatgc caaaagagat
     5221 actctacttt atactgccaa caaacttgaa gttaaggcta tcaaagaaga atataactta
     5281 agaaaagaaa atggatttga tgttgaatat attgatgaat ccacaaaccc ttttaacttt
     5341 gatttaaaat caggattgat agcaaagaat ggaggacgag aattagaccc atataaatat
     5401 agtcaccatt tgattgatgt aagtttaaaa aatggtcttc aagtatatga aaatacagaa
     5461 gtaaaaaaag tagatttatc aaatgataag gttactgctg aagtatcgta tggatacaaa
     5521 gttcatggta aaaaattaat tgtagctaca gggtacaata caagtttatt tacaaaaaga
     5581 aattttgcaa ctaagtcaaa tacttttaat atagcaacga aaccacttaa aaacatagct
     5641 tcttggaaaa acaacatttt aattagagat aactgtgacc catataatta tctaagaact
     5701 actaaagaca atcgattaat tataggtggt gaagatgtaa gttttgatga tatagaaaat
     5761 gaaactttag ccaatgaaaa gtatgatatt ttagaacaaa gattgaaaag tatgtttaag
     5821 gatataaaag acattgaagt tgaatataaa tattgtggtt gttttgcttc aactttagat
     5881 aatttaggtt ttattggtcc agataataaa aataacaatc tatggtattg cttaggttat
     5941 ggagcaaatg gtatactctt tgcaatatta ggaggtataa tgttaagtga attatatctt
     6001 ggcaaacaaa ataaaaatat gaaattattt aaagtagata gatttgataa atagatatta
     6061 tatattaaaa gactgtaaaa agatgaaact ttataattct aaaagttaac tttgatacag
     6121 tcttttataa tgttcctttt aaaaatactt tatttataat taaatataaa gtaaaatgtt
     6181 aaaatataac tgataaaatt tagtcataag ataatattta agtttatttg atgaaatatg
     6241 gaggaattat tatgaaggaa atactcgtta ttgctcccac taaaggaacc tatgaaaaat
     6301 caattcacat agttaaaaaa aacaaatata caaatattga tgttgtattt ggcaatctaa
     6361 aagaaggtat acctcttgct gaaaaaagta ttaatcatgg tactagaatt atcataagcc
     6421 gaggtggaac ttacaatatg ttaaaagcta cttacaatat acctatagtt gaaattaagg
     6481 tagatgctta tgatattata aaaagttaca aagaggtaaa aaattccaac gaaccatttg
     6541 gaataattgg atttaataat gtcatctacg gtttcgatat tatagaagaa atattgaata
     6601 agaaaataac tatgattgaa attgaaaaag aagaagaaat ttatgatgct atagaaaagt
     6661 acagaaagaa aggtataaac acatatatag gagataccac tgttgcacat attgttaaga
     6721 gacttaattg taagggaata ttaatcgaat ctagagaaga aaacatactt agagccattc
     6781 aacaagcaga acaaatactc gaagcaacca aggatgaaca aaaaagaaga ttacagatag
     6841 aagctatgac agattttgta catgatggaa taataacagt tgataaaaat tttaaaataa
     6901 cattatttaa taagagtgct gaaaaaatat ttggtataaa aaaagaaaat gcactttatc
     6961 ataatgttgt agatgttatc ccaaacacag ttcttccaca agtagttgag actatggaac
     7021 ctcaaattgg tgaaatacaa aatataggga gtacaaaaat tattacaaat agagttccaa
     7081 tcattgttga tggtcagatt caaggggctg tttcaacatt tcaagatagc aaggagataa
     7141 gttcttttga acatacaata agaaggagct tattaagcaa aggtctatct gctaaataca
     7201 cttttgatga tataattcat gctagtgaaa agacaaaaga ttgtatagaa cttgcaaaaa
     7261 aatatgcttg ttatgatact cctatgatta ttactggaga atctggagtt ggtaaagaac
     7321 tattttgtca aagtatacat aactatagta agcgtaaaaa tgcacctttt gttgctgtaa
     7381 attgtgctgc catacctcca tctctaatag agagtgagtt ttttggttat gaggaaggtt
     7441 catttacagg tgctagaaaa aaaggtaaag caggtgtatt cgaactagca catgaaggta
     7501 caatcttcct agatgaaata agtgaaatcc ctcttgaact tcaaggtcga ttattgaggg
     7561 tattgcagga aaaacaagtt atgagaattg gtggtgataa agttattcct atagatgtaa
     7621 aaattatctg tgcttcaaat aaagacttaa aaaatatgat gcgcaatggt ttatttagac
     7681 aagaccttta ttttagaata aatatactaa cattatatct tccttcatta aaagaaagaa
     7741 aagaagatat tataaaatta tcagaatttt ttatacataa atattcaagc aaatacaata
     7801 aaacgccttt aattatcact ccaaatatta aaaaggcact tcttgaacga gaatatgaag
     7861 gtaatataag ggaattggaa ggtatgatgg aaagggctgt tattgtggaa tcatttgact
     7921 cgattttatt agaaaaggtt agcaatgaat atacaaataa tatagttgat aaagataata
     7981 gtgattttga atgcagtaat ttagatttaa aaactttaga aaaaaaatat atctataaaa
     8041 tttataacga aaataataga aatactacaa aaacctgtga aattctaaag ataactcgct
     8101 ccactctatg gcgtaagtta aaagaaatag aagatgatgt ttaaaaataa tacataccta
     8161 tatatatcaa aacgttttat gtttaatttt aatacataag acgtttattt gttttcaata
     8221 aaaaatacaa ttatatgata aaaagaatct ttgtaagtga aattctcgtc aatcgtactt
     8281 ttaattataa gtaatctaca gcatttcaat cgattattaa aaataatcat gataactaaa
     8341 gatacaaact ctatatattg gcatattaat tgcttttata atacctatat aaaaatgaaa
     8401 gggagtgata ctagatgcac atcttaaaat cagtgaaaaa gataccagga ggacttatgg
     8461 tctgtccctt gttattggga gcactattta atacagtatg tccacaagct cttgaaattg
     8521 gaggattcac aacatcctta tttaaaactg gtgcaatgag tatacttgct ctattttgtc
     8581 tatgcaatgg agctcaaatc aatatcagac aagctggtaa accattgact aaaggtatag
     8641 tacttacagc aacaaaattt ataattggtg cagtattagg tattattgtt agtaaattta
     8701 ttggacctaa aggtataatt ggtattacac ctcttgccat agttgctacc atgactaatt
     8761 caaatggagg tctatttgct gctcttgcag gtgaatatgg agactccaca gatgttggag
     8821 caatatctat cttatctcta aatgatggtc ctttttttac aatgcttgct tttggagtaa
     8881 ctggacttgc aaatgtacct ataatagcac tatttgcagc acttatacca atactacttg
     8941 gatttatact tggtaacctt gatgaagata tacgagaatt tcttgcacct ggtacaacat
     9001 tattaattcc tttcttcgct tttcctttag gagcagcatt aaactttaat caaattataa
     9061 ctgctggact acctggagtt gtacttggtt taggagttac tataataaca ggtatgggtg
     9121 ggtactttgt aatgaaactt atacgtgcag aacatccagc agtaggtgca gctacaggta
     9181 caacagccgg aaatgcagct ggtactcctg aagctttagc agctatagac cctactctag
     9241 ctacaatagc agctgtttca acagtacagg ttgctgctgc tattattgtt acagcaatat
     9301 gttgtccaat gttagtttcc tatctagata aaagagaaaa aaagaaaaaa tctattttta
     9361 aaacaaataa aaatattgaa gtaaattaat acttaaggag gtaaaattaa tgaatgcaat
     9421 tattcatgca aatgaaaaag ataatctagt tacatgtgtc agacctttag ttaaaggtga
     9481 aaaagtttca attgatggaa aatcttacat agtaaatgat aatataccag tctttcataa
     9541 gatggctaca gaagatgtaa aaaaaggtga tgttgtatac aaatacggtg aaattatagg
     9601 aattgcaaca gttgacataa aaactggtga ctatgttcat gtacataaca ttgaaagtac
     9661 acgtggtcgt ggagataaaa aataggagga agttaaaatg aaattaatgg gttatttaag
     9721 agaaaatggt cagtttggta ttcgtaatca tgttttaatt attccaacat ctgtatgttc
     9781 aagtgagaca gctacaagaa tagcatcact tgtgcctgga gcaatagcaa tccctcatca
     9841 acatggatgt tgtcaaattg gttctgatat agagcttaca gcaaagacat taataggatt
     9901 tggaaaaaat cctaatgttg ccgcagtttt agtagttggt cttggatgtg atggtattca
     9961 agcaaaagaa ttagcatctg aaatagctac aacaggaaaa aaggtagatt acgttgttat
    10021 acaggaatgt ggcggaacat taaaaacagt ttctaaggga gctgaaatag taagtaaaat
    10081 ggctcgtgaa gtatcaaaag aagtaagagt tgaatttggt atgagtgaaa ttacattagc
    10141 acttgaatgt ggtggttctg acccaacaag tggaatagct tcaaaccctt caataggcgt
    10201 tgcatcaaac cttcttgtag atgaaggtgg aagtagcata ctaagtgaaa ctacagaagt
    10261 aattggtgca gaacatttgt tggctacccg ttttgaagat gaagaaatga aagataagtt
    10321 tcttaaattt gtaagtgatg tagaaaaaag agcaattgct atgggagaag atttacgtag
    10381 tggtcaacct actccaggca ataaaactgg tggactttca acaattgaag aaaagtcact
    10441 tgggtgtatg tataaggcag gcaataagcc attcaaaggt gctcttgaat atgctgatat
    10501 tgtacctcct gataaaaaag gattatattt tatggatact ccaggtcaag atatagattc
    10561 tatcactggt atggtagcag gtggtgcaca aattgtaatc ttttcaactg gtagaggtac
    10621 tccaacagga agccctattt caccagttat taaaatcact gggaatagtg atacatacaa
    10681 caaaatgcca gacaatatcg atataaatgc aggtagaatt ataacagatg gtgctaaaat
    10741 agcagatata ggtcaagaaa tatttaatga aataatagaa gtctgtaatg gtaaacatac
    10801 caaagctgaa agtcttggtc atagagaatt tggcatttac agaatttcat ctacatttta
    10861 gattgtaaca aaatatcttt tataaatata atacttatgt agatacataa gctaaaagtt
    10921 ctttctaata ctataaaaat tttctatact gaaaaggaac tgtatcaaaa ttattttgaa
    10981 aacagttctt tttttaacct cttttactca aaatattata aaataagatt taaacaataa
    11041 acgtatcttg attaatctct aagaagacat aaataaagct aaatgtaaag tgggataaat
    11101 aaaatttaat atctttgtat gaaaagatat ttggtataat atgtctgaga gaattcttat
    11161 ttgtaaacaa ataaaggatg gaggatgaaa caaaagaatg aactttgaaa acacaaaaaa
    11221 taaaaaaata atagctactt tactattgac acttctttta atcgtgtcat taacatgtgt
    11281 cacttttatt atgaatctaa ataaagtaac acataatgaa acagaacgat atcttgtaga
    11341 agtttcacaa ggtatttcca ctacaattaa tgtgaaatgt aaaagtatat ttaggacact
    11401 atattctatg gggagatact tattttgaga ttaatgatag tgaaaaagaa gtcaatagat
    11461 acttatcaaa aaaattaaaa ttatttgaat ttgattggtt aggtattata gattctaatg
    11521 gaaattttgt aagtgccctt aaagatacag gtaatgttaa aggacaagac ttttatagaa
    11581 aagcccttca aggcaaagaa acaattactc ctgttaaatc acaatctggt acatatggaa
    11641 ttttatattc tgtaccacta tacaatggcg ataaaattgt tggagctgtt gctgcttgga
    11701 atactattga tactatgaga aatttattaa gtgtagaaag ctttcatggt gaaggatttt
    11761 cccaaattat cgaccaagat ggaaatttta ttgtaagttc agcaaacaaa aatgctccaa
    11821 aaaatgtggt aaattttttt gacatgatta aacaccaagg aaaacaggtt gataaaaata
    11881 aacttgaaga aatgaaaata aacttaaaaa agaacaaaac tggttctatg tattatacat
    11941 taaatgatgg tatttctaag gcaatgcact atcttccatt acaagaagaa aacttatacc
    12001 tcctttcaat tgttccaaca aaaacagcaa gtgcacagtt tgatttactt ctaaaaaaag
    12061 caatcttcat caatatttta attgttatac tatttttatt gcttattata ttgattatgt
    12121 ttataaatca aaaaaacagg gcacaactta taaaaatcgc ctatgtagac tctgtaacta
    12181 aaggttacag taaagcacgt tttgatattg aagctaaaaa aattatacag tcatcttcat
    12241 cagaaagcta ttcattggta tctataaata ttcagaaatt caaacttatt aatgattctt
    12301 ttggtagtga agcaggagat aaaacattaa agtacataca tgatgtcatt agtaattatc
    12361 tagaaccagg agaattgctt tgtcgagttt ctaatgacca gtttaatgta cttattaaaa
    12421 ctattcctca aaaagaaatt cttaagtaca tagaaaatat caccaatgat attaatagtt
    12481 tcaataaaaa tgaagaatgt aaatatttta tagctctatc tataggtgta tataaaattg
    12541 atgacccaaa tctaccttta gtttcaattc aagaccgtgc aaatgttgca cgaaaaaata
    12601 ataaaggaaa cattggaaat catccacttt atacatgtgt tttttatagt gatttagagc
    12661 gtttaaaaat gattaaagaa aaagaaatgg aaaatagaat ggaggaagca ttaaataata
    12721 acgaatttat agtatatctt cagcctaaaa taagcctcaa agataatact attgtaggtg
    12781 cagaagcatt agtaagatgg caagacccaa ataaaggcct tataccacca gacgagttta
    12841 ttcctttctt tgaaaaaaat ggttttatta caaaactcga ccactatgtt tttgaaaaag
    12901 tatgtagtat gataagaaaa tggatagata atggagaaac tccaatacct gtttcagtta
    12961 atttttcacg tgctaattta aataatatag attttttaga aaaatacaga atgcttcgta
    13021 ataaatacaa cattcctgct aagctcttag aaattgaatt aactgaaaca cttgtatttg
    13081 aaaatctcca aaaattaatt aatataatag accaaatcca tgaagaaggt tttcaatgtt
    13141 cactagatga ctttggaagt ggatactctt cactcaatat gttaaaagaa ataaaagttg
    13201 acacattaaa acttgaccgt gcttttttta gctcacctaa tgcagataat attgctgaaa
    13261 atcatgtaat agaatctata gttaatttgg caaaaaaatt aaatatgaat agtatatcag
    13321 aaggtgttga aacaatcttg caaatggatt atcttaaaaa aattaattgt gatatgatac
    13381 aaggatatgt tttttcaaaa cctctacctc aagaaatatt tgagaaaatg acttttggaa
    13441 aaaaactaga agaaaccagt atggatagtt ccgtttaaat aaaataaaaa acttgtctct
    13501 ataaaataat tttaatcatt ttataaagac aagtcttttt attttccaat ttaattttat
    13561 tttctaaatt tcttacactc ggcttgtttt aaattccctt tttaagaata ataatacgat
    13621 tatcatagca ataaaatctg caactggctg tgccaaccaa acaccatcta aattaaattg
    13681 aattggaagt acaatgatta atggtataaa taaaataaat tgtcttaaaa gagttaaaaa
    13741 cattgaatgt ttaactttac ttatacactg aaaatacaca ggtcctacaa tagaaatccc
    13801 cattataggc aatgtaatta gatttaggct tatacctctt actgctatat ccatcaaatc
    13861 tgaatcttta ttaaatatac ctacaaatac ttctgggaaa acttgtacta ctaaaaatcc
    13921 aaatgacaat atgacaactg acactatgat agataatatt aaagttttct ttgcacgctc
    13981 atattgtttt gcaccataat tgtatgatat tatagtttgc ataccttgat tcaatccaaa
    14041 cacaggcatc ataaacatca ataaaataga tgtaagtgct gtcatagctc caatagctaa
    14101 atctccacca taaaccttta gagaattatt tgtaaacatg tgagttatac ttatagctac
    14161 ttccatagca aaaggtgtca ttcctattgc aaataccttc tttactatat ctttattcaa
    14221 ttttaagtta cattttttta gttttaaatt ggacttccct cttataaaat aatgtattac
    14281 ccaaaaaaat actactattt gacagagtac tgttgctata gctgctcctt ttatacctaa
    14341 attaaataca aatataaata tagggtccaa tatcaggttt aatacacatc ctacaatcat
    14401 tgtccttgct gctaattttg gattaccttc tgccctaatt gcattattaa gagaaaatgc
    14461 aactaaatta aatatacttc caaccaatat gacactcata tagtcttttg cataaaatat
    14521 tgaatcttta cttgcaccca aaataaaaag tatctcttct aagaaaacta atcctaatat
    14581 agttataatt aaagagataa ttatagataa tgtcaaagca ttacctaata tcttctctgc
    14641 ttcttctcta tttctttccc ctaactttat agacaatctt gtgctagcac ctactgatac
    14701 caacattcca aaagctatta ttagagtaaa tactggcata gttacaccaa gacctgcaat
    14761 agctatagag ccaatacctt ccatagaacc aataaaagct ctatctacta cattataaag
    14821 tgaagttacc atcattgcta ttattgcagg tatcgaatac ctaaataata aaatccatat
    14881 tttttcatgt cttaatgctt cttgattctc catgttgtca ccatttacac catgtcataa
    14941 gttatacaaa ttggtttatt tgttcttggg tctgttgcta tttctgcatc aatattaaat
    15001 aattctttta gattatcctt cgtcataaca tcatatgcat caccttcaca aactatttta
    15061 cctttcttaa tacctatcat gtggtcagca aatcttgctg cattattaag ttcatgtatt
    15121 accatgacaa tagttcttcc ttgtttttta tttagttcat ctaataattt tagtatctcc
    15181 aattgatgtg ccaaatctaa atatgttgta ggttcatcca gtaataatat atcagtttct
    15241 tgtgccaaag ccatagctat ccatgccctt tgtctttgtc ctcctgataa atcttccata
    15301 tttctatcct taaattctgc aattccagta acttctaaac tccaatttac aatctctctg
    15361 tcttctttac tagaattacc aaatccactt ttatgaggaa atcttccata agaaattaat
    15421 tctgaaacgg taagaccact aggagcttga gggctttgtg gaagtacagc catatctttt
    15481 gctatttctt tagacttctt tttgtagatg tcctctccat ttatatatat tttcccacta
    15541 ttagggctaa taattctacc tatagtttta agtattgtcg attttccaca accattagcc
    15601 cctatgatgg tagttatttt tccttttggt atatttaaat ttaaattttt tataatatct
    15661 gtgtttccat atgatacatt tagattctta gtatttatac agttcataac aacacccttt
    15721 cctaatcatt taccaacatt aaataaataa agtacggcac tcctattata gaaactacta
    15781 tacctactgg tatttctata ggtgctagaa gatttcttga tatagtatct gctattaaaa
    15841 gtaataatgt tcctatcaat gctgatactg gtattaatct tttatgcttt ggtcctatta
    15901 tttttctacc tatatgtgga gctattaaac ctaaaaatga aatactacct gctactgctg
    15961 tcgatacacc agataaaatt actgcatata ttataagtct tcttctttgt ttttctacat
    16021 ctacaccaag acttatagat acttcatcac ccaaatttaa aacatctata tatttagatt
    16081 tataaatagt tatacccata aaaatcacta taaatggaag cacagctatt acatatttcc
    16141 aacttgttcc ccaaatgctt cctgatgtcc atgccataac cctattaaat tcttgtgttg
    16201 taaatttcaa ttggaatatc gtcaaaattg aagtaaaagc tatgtttaca cctataccaa
    16261 taagtagtaa tcttgacgaa tttataccac ctttccatgc aagagaatat ataagaaatg
    16321 ctccaaataa agctcctata agtgcaacaa taggcattgt aaatacagtc aagttactca
    16381 taccttcata tacattacca ttcataaggt atatataaac tactacaaac aatgcagcac
    16441 ctgaatttat tcccaaaatt ccagaatcag ctaaatcatt ctttgtaact ccttgaagta
    16501 ttacacctgc tgttgataat gcagaaccaa ccaaaagtgc tattactatt cttggtaatc
    16561 ttaatttaaa tattgcaatt tcatgattac gctgaccctg ccctaaaaaa gtctttataa
    16621 catccattgg ttctatagaa aatgagccca tatttaaact caataaaaaa gtgataaaaa
    16681 taagcaccat taaaattgat ataacaagta atgttttaga atttattttt ataatatttt
    16741 tcatagtacc tatttctccc cctttctaac aaggtatata aatattggca ctcctataag
    16801 tgctgttata gaacctactg gtgtctcata tggaggattt atcatcctac ttaatatatc
    16861 tgtatataca agcaatacag ctcctaatac taaagagctc ggtattatat acttataatc
    16921 tgaacctact attcctttag ctatttgagg aactataaga cctataaaaa taatattacc
    16981 agacactgac acagatgctc cagtcattaa tattacaagt actatacata taaatctaac
    17041 aaacttagtt ttttgtccta atcctattgc cacttcttct ccaagactta atatagttat
    17101 tcttggagcc ataataatag ctaaaataaa tccaatacca ccagctacaa atagcaattt
    17161 tataccttcc cacttcactc ctacaagccc tcctgatatc cagaaactta attgttgaga
    17221 taggttaaaa tacatagata ttgctgatgc caaagaaatt agcaatgtac caagagcgac
    17281 tcctgcaaga gctaatttta cattattaac tcttcctctt gatttagaac ttataaaata
    17341 tataaataat ccacttatac ttgctccaat aaatgcaaac atcattacac taaaacttcc
    17401 atgtataagt tgaggaagtt ttttttgaag taccagtgat atcgaaattg cgaatgtagc
    17461 tccttgtgta attcctataa cagatggttc agctatagga tttctcgtta ttccttgcat
    17521 tattgcccca gaaactgcta aaaaccctcc aacaagtgca gctgctattg cccttggaag
    17581 tctaacatcc tttactattc tcatatttat tccatcttcc atttttatca aactatctat
    17641 aacagttgat agattcatat cttttgcccc aatagttatg gataatacaa ttccaaatac
    17701 aagtacaact atacttacta atatcaacaa ataagttata ttttttttac tcagcttagt
    17761 caattatcct acctcctttt cccttttaat cgtcagtata atatcatcta ttaagtattt
    17821 taattttaag atttattaaa aataggtgtg aaaaatgaat atcattatct cacacctact
    17881 aagttaagaa ttactttgtt aattcatttt taactgattc taataataac tcttttccaa
    17941 ttggattgta tgattgactg aaaaatggag cagcatcaag tattgttaca ttaccatctt
    18001 ttactgctct tatttgagcc cagatagcac tattttctaa atctttttta tctgcttctg
    18061 tagcaattac aactatatgg tctgcatcta tatctgttaa accttccata cttaccattg
    18121 gtagtgttat tccatcctgt tttggcatat tcttaggtct agctaatttc atatcatcat
    18181 tgattagagt acctattcca ccatcactaa ataccataaa ttgacctgaa cttgccaaca
    18241 ctggtaaata agttttttct ccattctttt ctataacttc cttacctaac tttgtagctt
    18301 tttcatcata cttctgtagc caacttttat cttcttcttc tttgtcaaat aactttgaaa
    18361 catcagttaa cttgcttctc cagtcatttg cataatcttt catcataact acaggagcta
    18421 tttcttttaa ttggtcatat attttttctt gtctttgact cattattatt aaatcaggat
    18481 ttacttctaa tatagcttcc atatccatag tatccatcat tgagtgccca actatcttta
    18541 cacctttaag ctcttctctt atgtaacttg gtaatttatc agtttcatat gagtctacat
    18601 ttgctgttgc aacaggctta taacctgcta ataatagttc ttcactactt cctgatatat
    18661 caactatttt tttaggattt gatggtatct taacctctcc tttaacagat tgtacaactc
    18721 ttgtctctga tttatcttta tcctctgtat tcttatctga acatgctgta acaagtacta
    18781 acgtagttat aatagatata aatattgcaa gtgattttat ttttttcatg attacttctc
    18841 ctacttgttt attatactta catgatattg attatcataa gcacatattt attatagcat
    18901 ttttctattt ttagtcaata ttattttgta aaaggataaa aattcctaat taatcttaat
    18961 tatatatagc ttagataagg ttttcttata ttaaacagta ttttttttaa gaattggaca
    19021 caaatacaat gacttttcag ttaaacaatg ataaaatgca atttatagat aaggaattta
    19081 gtagggagga ttcaaaacat ggtacattta gtatattgtg ataatgcagg aaaaaaagga
    19141 gaaaaggtct tagataaaat cttggatgga acaaaaacaa tggttattag aggtgctgct
    19201 ggtagaaaaa taccacatag tagagtattt gaagatgagt tactttattt tatggaaaaa
    19261 ggaactgcaa aaatatctgc aaaagccact gttaaaaagg tagaaaacta tgttaaatta
    19321 acagaagatg aaatttctaa aattttaaat gaaaatcaac caaaattaaa tttatctgaa
    19381 aagcaaaaag ttcgttggca taaaaaatgt ttatgcttag ttgaatttga aaatgttgaa
    19441 gaaataacac ctcttgattt tgaccatcaa ggaaatatgg atgattggct tattgttgaa
    19501 aaaattgaag atgtagttgt tggtactagt ataccttaca attatgagaa gtctaaattc
    19561 taaagtatca ataacataat actatattgc taaactctac ttaaataaaa aaagagtgca
    19621 tttaataatt ataaaaatta tttttataca ctctttttta ataataattt taaattactt
    19681 actttctaat ctttcatata catctataaa ttcttctgct aattgttgaa aattcatagc
    19741 tgtcattaaa tggtcttgtg catgaataag tataacactt atttcagttt tattaccatt
    19801 agcttcattt tgtatcaatt ctgtttggaa tttatgagct ttatgaattg attctttcga
    19861 cttttcaata cattctctag ctttatctat attcccgctc tttgcttctt ttattgcttc
    19921 aaatgctagc cctttcgcat cacctgcaaa tccaattatt ccaaaagata tctctatcat
    19981 cttctcatcc atttattcga cctcctaaat atctctatag cttgatatga ctaatccatt
    20041 cttttctaaa aattctttga cttttttaga tgtaagtatc ttgtgctctt tcgttctatc
    20101 aatagcataa gatgtagaat ttaaaatata atcatctaaa aaagctgggt gagacataat
    20161 atccactaca tcatacttca ttatttcgtc tatatttttt ataaaatatt cttcacttac
    20221 attttcctta taaaaactat ctattaaagg aactacttta gaatactcta aattatattc
    20281 aaatccacct cttattggaa gattatactt ggttacaatc ttctctatta ctggctttaa
    20341 acttactaaa gtatggatat gatggtgact atctaaatga gatatatcta ttccaagccc
    20401 ttttacccta tctatttgag cacaaagttc tctataaatc tcatcacaat ccattttttc
    20461 aataatttcg tctgtaatcc tcctaataaa aaaaccgtct ttatcaacaa tagtttttaa
    20521 gttagataat aatggtctac cacaacttaa agtcatatga actccacaat ttaaagtttt
    20581 attttcttta agtaagccta ctccatgttc taccccaggc atatttgcca tcatagaagt
    20641 agacctaact ataccattat tatgagctga aattattcca tagttcacag cttcacaata
    20701 tccaaaatca tctgcattta ttataatttt agtcatacca taacctactt tctatagaca
    20761 actatctatt aaatctagga agataatctt tatgagcttc caacatttcg tctaatatag
    20821 tttttgctaa gtcatcagat ggaatcaatg ggtttataca aagtgccaaa agagcagatt
    20881 cataatcgcc atatacagct gctttagctg ccaatacttc aaatgatttg atttgactta
    20941 ctaatccatg cacagaatct ggtaaatatc ctatacttat tggttttgga ccattttttg
    21001 ttattacact acttacttct actgcttgtt catctttaaa atctcttata gaacccttat
    21061 ttaatgtatt aactacttgg atatcttttt tgtcattgta tatagaactt attaaattac
    21121 aagcagcatc actatagtaa gcccctcctc ttttttcaag ttgaggtggt tttacatcta
    21181 atttttcatc cttatataat tcaaataatt gttcttccaa ttctttaact acttgacctc
    21241 tagcttttcc ctctttaaac tctctcaact catcctcaag catttcttta gttttatagt
    21301 agtatctatg gtatggacaa ggtattgcac ccaaagattt tacaaattca gcattaaaat
    21361 caattgcctt tatattttgc atacttatat ctgctttgac aaatttatca attgcttctt
    21421 ttgtaacatc ttctccatct aaagatacat ttagaccata aaccatatga ttaagacctg
    21481 caaaatccat acttattctg tcactttcta cattaaatag tttggcaaca tcatttttta
    21541 aatgaattgg cacgttgcat agacctatat atcttctaaa attagtatat ttgaatacag
    21601 cctctgttat tattcctgtt ggatttgtaa aattcaccat ccatgcattt ggacataatt
    21661 cttccacatc ttttattata tcaaatataa ctggtattgt gcgaagggct ttaaataaac
    21721 ctccagcacc attagtttcc tgccccataa ctccatgaga taaaggaata gtttcatctt
    21781 ttattctcgc atctaaaaga cctactctaa gttgtgttgt cacaaagtct gcatctctta
    21841 gagcttctct tctatctaaa gtaagattta ttttcatatc tactccagct ttttttacca
    21901 tcctcttagc tagatttcca actatctcta atttttcttt tccagcttct atatctacaa
    21961 gccataattc ctttacaggt aactcttcat atctatttat aaaaccctct acaagctcag
    22021 gagtataact tgaacctcct ccaattgtaa ctatttttag tccctttttc atctcattat
    22081 accatcctta cctataattt aatatactct acaaaacccc atacatttat ttaaaactct
    22141 atacatttat ttaccgaaat aaaactacct agttgttaat tttcaattta ttagcttcca
    22201 ttttttcaga cgctatgaca aatggaatat atattaatat tgataatatt aaattaaata
    22261 gtgctaaagc tccgccagtc cagtcacctg tagccattgc tccacctaat ataggaggtg
    22321 ttacccaagg tattttaggt aaaattactg gtgctactaa tccaaaatct attgctaagt
    22381 atgatacagc actacatatt actggtgcaa gtacaaatgg aatcataaat ataggattta
    22441 aaactattgg aagtccaaat atcaatggtt ctcctatgtt aaatattgca ggtggaccac
    22501 ctaatactat catctcttta cgtcttcttc cagctattaa taaagctact attaatccca
    22561 ataccattcc agaaccacct aaatatacaa atgcatcaaa gaatgcacct gcaagtacat
    22621 ggtatccttc cgttgctcct gcttgagcca aggcagcatt ttctccacct aatttcataa
    22681 gtatagtttc tgtaaaagga agtgctatat ttgcaccatg aagtccaaca gtccaaagta
    22741 tatgaattat aaatgcaata agcattgtag aacctagact atcagctaca tttgatagtg
    22801 gtgctcctaa ataagtattt aaaatatctg ttaagaaact tccattagaa agcattttta
    22861 taaatattcc tattacagta aatatcatta tagttaacat tcctggtatt aactttgcaa
    22921 atgatcttga aactgctggt ggaactccat ctggcatttt tatagttaat ttctttacct
    22981 tagataatct tacaaatatc tcagttgata ataatcctat taatatagaa gagaataaag
    23041 ctgctgtacc taagtaggtt tgttgtatca ttccccatcc ttcaactact gtagttcctc
    23101 cctcaggaac tattttacca atttgtggag ccattataaa aaagatagat aaagctataa
    23161 gcccagattg taacccatca ctctcataag attttgctag aaaataagca acacctatta
    23221 ctaagaatac tgccattgta cctattgctc cccaccaaag gtcgcctcca aatgtggtcc
    23281 agttttctcc aaatatactt gccatcaaat ttttgtatgc ttccaatggt aagttattta
    23341 tcaatgttcc caatgcacct accatagtta taggtatcat tactataaat gcatctctta
    23401 ctgctactaa gtgcctttga gctcctattt tagcagctac aggcactata tatttatcca
    23461 taaatgacat gaatttttcc attttacatc ctccagtaca actttatttt gaactattta
    23521 actctacagc tctatctaac acagcttttc cattacatct tccataatcc atcatattta
    23581 taacttctac tggaatatta tatggttttg ccatttcagt tgcttttcct aatacatatc
    23641 taacttgagg ccctaaaagt aagacatcac aattttctat ttcatttttt aagttcactt
    23701 ctgatatagc ccatattttt gcttctatac cgttttcctt tgctgcatcc tccatttttg
    23761 taactaataa actagttgac attcctgctg aacaagctaa cattattttt atcatttttt
    23821 cccctccaaa ttagattcat taaatatttt ttaagtcata agttttttaa actatacatt
    23881 taaaatatgt ttttctatta attttttctt aatgtatata tcttattttt aaatgtatat
    23941 acatttaatt cttgatatta ttatatacca aatgtatata catttcaaga aaactttttc
    24001 attttattta aattttattc gacaacagca ttcaaagtta atgtatatcc atagtgatat
    24061 gtgtgtgagt actcaaaaat ctcactattt tgtaacttac aaatttggct tatatataaa
    24121 agtggctctg gttcttctaa ttttagatta tccatacact cttcattcga tattacagct
    24181 tgtacttctc tctctgacat ggctatcttg tattcacaca cttcttcaac aaaattataa
    24241 agagaatttt cacaatgagt cttatttaaa tttggaaata acttaactgg catataagta
    24301 atcatatatg aaactcttgc atcattggct aatcttactc tttttatttc ccaaacattt
    24361 tcttctaaac ttatattcaa cttacttgat aaattttcat ctgcctgaac tatttcaaga
    24421 gataccaata tattagatac tttatagcct ttactttcca tctctttagt aaatccactt
    24481 acagacgata tattatcata tatattattt gcaataacta tactacctac acctctttgt
    24541 ctagttatat agccttcttt aactagatta ttaagtgcag accttacagt cattctagtt
    24601 acattaaatt cttcactcag ctgtttttca gaagggatta aatccccttc tttcaaactg
    24661 tctgaattta ttaactctct aacatgattt tctataactt tataaattgg ctctttcatt
    24721 taattcactc cttattaaat cttttatata ataagataat tttatataca aacaaagaaa
    24781 atgtctatac atttaagata atttaaattt tattcagaaa tcttgtacaa ctttccgagt
    24841 tttaataaag atataaatag acttatcatt aatatacctt ttaatatact tgataaggtt
    24901 atactccacc atacaccatt tagacccaac atttctggtc tagatataaa gtaagccaat
    24961 ggtactcttg caccagtcag tatagtaatt ataatagaag gtatataggt tctccctaat
    25021 cctttgaaag ctcctgttgt tattatttct aaacacataa aaaattgaga atagcctaat
    25081 attttaagat aaactgctcc tttttctatt gcctcacttt catctataaa aatagaaaat
    25141 atactctctc ctgcaaacac taatactaaa gtagttatta taccaagtac agaagataca
    25201 gttatagcta ttttgcaacc tttatttatt ctagaatact tcttggcacc ataattttgt
    25261 ccaacaaaac tgcctaatgc aactgatagc ccttcagccg tcatccagga aattgcttct
    25321 atttgtgagc ctactttttg tactgcaaca gccacaggcc cccatgatgc tactattact
    25381 cctaatagca ttgagaatac tgtaaacata ccactttgta ttgctatagg taatcccaat
    25441 ttatacagaa ctttataata tttagtctct atttctttaa atagtcttaa tttaaaatat
    25501 tcttctttac ttttaactat tatatatata aaacatgtag taactactat ctgagcaaat
    25561 acagttgcaa tagcagctcc tgcaacacca agttttggag caaatcccca tccaaaaatt
    25621 aatatagggt caagtataat attagttatt aatcctactg tattaatacg aaaaggagtt
    25681 ttactatttc caagaccatt gaaaattgca gtaaatactg gattaataaa ataaaatacc
    25741 atacctagag ctactattat caaatactgt ctagacatgg ttataacttc taagtctcct
    25801 aatctaaaaa aacctatcaa ttgtttatta agtactatta aagaaattgt atatattatt
    25861 gctagtataa tatttatttc tattgctgac ttcatatatg atttagtagt gcttatatta
    25921 tttgcaccaa tactttgtgc tacttttatc tctccaccta cttttgaaat cataataaat
    25981 gccattgcaa gccatggaaa aaaaccagct gttccaactg cagctactgc tttacttccg
    26041 gctttaccaa cccatatcat atctatcatg ttatacccca tctgtataaa tgaagtcccc
    26101 attattggaa gtgatagctt aagtaatttt tctgtaattc ttccttctgt taaattaagt
    26161 ctgtctttca ttttccacct cttttatatt aaatttattt gcttatatag ctagattaat
    26221 cattttattt gattgagttc actatccttc aatttatatt atcatatctc aggattgcat
    26281 ctcaatcata cttatgttta agatacagta ttttacctat ctatgataaa attaatactt
    26341 atgaatataa atctagccat ttcatcaata catattctaa cccttaactt aacttcaatt
    26401 aagtatacat atcgtaacca attagaatat tttatacggt atcttccatt aataaacaac
    26461 caatatcaat gatttatata taattttatt tatacataaa atagtatact ataataataa
    26521 aaaaactata tttgaatgct ttaagcatgt ttactaattg taatttccac acaaatttga
    26581 taatatatga atatattatg gtgaaattta tagatatata ttaattatgg ggagattgtg
    26641 ggaggttctt ttcacaagac atttaagcta tgaaaataaa taaaaaattc aatagagttg
    26701 atatgcaggt ttcaatactt actgcaatta ttgtaataat atcttcctca tgtgtatatt
    26761 tttttaatta tacactaact tataagagca tgatatactc tttaagtgag aggtcaaaac
    26821 atatctatga atatgtagaa agaaatattg ataaaaatac ctttactgaa attaattcta
    26881 aagaagacca atcaaaaaag tcttataaaa gttctaaaat ccttttagaa tctgtaaaaa
    26941 atactactgg agtcatgtat ctatatactg ctaaaaaaac aaaagatgga tcactagtat
    27001 atgttgttga tggtttaaat tcatctagga gtgattttag aaatgctggt gatttgatag
    27061 aaaaagagat ttggggagac cttaacaaag cattagataa tcatatattg ttacctaaaa
    27121 agattaaatc aacatcttgg ggatacatat ttatatcgta ttttcctatt cacaatgatg
    27181 ttggtaaggt tgttggagta ataggaattg aatttgaagc aaaacatcaa tacaatacat
    27241 ttaaatttat aagtatagtt acaccaataa tagcattatt aacttgtcta atatcagcat
    27301 tgattgcagt gattctattt aaaagaatat ctaatcctac atatactgat tttgctaata
    27361 cagattattt aacaggccta aaaaatagaa atgcttttga aatagatatg aataacttaa
    27421 acaattcaaa tctaaagaat ctaatatcta tcatatcaat agatttagac ggtttaaaaa
    27481 agattaatga taaatttggc catcagacag gagacttata tattaagcaa gcaagtaaaa
    27541 ttatacaata tgtaataaat aaaaaatata tagtttatcg tattggaggt gatgaataca
    27601 taattattct aaaaaattta tctcacaaag aaataaataa tattataaat aatattaatt
    27661 taaaaatagt tgaagaaaac tcaactaatg aattaaaatt atcaatgtct attggatatg
    27721 ctatttttga tgaaaaagta gatgtttctt tatttgatac atatcgtcgt gcagattctc
    27781 aaatgtatga taacaaaagg aaaattaata aacaatatca ataataaatt taaaaagtct
    27841 attctttaaa taataaatca caagaataga cttttttaaa tttgtgtttt tataagataa
    27901 attatttaat taaagtttgt tcttttttct aaacgattct ttataaattc atatattact
    27961 ttcactaata tatttgtgac taatattaac acagctagtg ctgctgcctg tgcagtactc
    28021 cccttatcat ctagttgtac aattgatatt gcagctactt ttgaacttgc agtatatata
    28081 aaaactaatg ctgatattgt caccatggag tttaggaaaa agtacataaa gttctctaaa
    28141 actgctggca aacaaagtgg tatagttacg ttcttaagaa ttgatgcgct gtttattccc
    28201 attgatttag ctaccatatc taattcttta tctactttct taatagcagt agtagctgtt
    28261 ataaatgcta ctgaaaagaa atgaactata ttacatataa ccattataag taatgttcca
    28321 tataatccat taaacaaatt cttaatgtat attgtatcca taaaattaaa ctctaattta
    28381 ttaaaaaata atacataagc tatacctaaa actaatcctg gcaatgccat aggcaatgta
    28441 cttaaaaaat acccaaatga ttttaaatat ggagcttttt ctgttttttc attcaaatat
    28501 gcaaataaga aaacaaagct cgtccctaga atagctgtaa atagtgacat ttttattgaa
    28561 tttataaatg gtttaatccc atcaatactg tttttcatag aaaaatgttc cagtgtcagt
    28621 cttaaatcat atggccaaac tgatattaat gaagataaga ttacagttaa cattacagct
    28681 attataccaa atgcaataat tcctacaacc cctcctaaaa tactatctct taacttatta
    28741 gttttaacct tatatggaat tgactttgaa gttatatatg aattttgatt tttttcaatt
    28801 tttttagtta ctataaacga tattattgca ggtattaaaa gtaatattga tacagttgct
    28861 cccattggca tattctgttg ccctataatt tgcttatata catctattga taatacatta
    28921 aactctcctc ctatcacctt tggagctcca aaatctgtaa aacataatgt aaacgatacg
    28981 aatgcagaac ttattaaaga gtattttata cttgggattg ttatgcttat aaattctctt
    29041 atcttgctta tccccatagt attactagct tcatatagtc tataatcggt tgaacttaga
    29101 gatatataaa gtatttgaaa tgattgagga aatacatata taatttgtgc caatattatt
    29161 cccactgctc catatattgg aacatgcact ccaagtaaat tagttaccaa accttgattt
    29221 ccaaataaat atactagacc tattgcatgc atcattgttg gagcaaacat aggaaacata
    29281 gctaatgatt taaaaaaacc tttaaattta atctttgttc tagttaatgc atatgcatat
    29341 actaatgcaa gtgttagtga tattacagtt gatataactg atacaaatac tgtatttttt
    29401 agagaattta atagagattc agaagccaag tattcttgaa agttttgtaa tcctacaaag
    29461 actccatctt tatctaaaaa agcctgtaca aatagatata ttataggtgc taataaaaat
    29521 attactaaag acataactaa tactgtaagc atacattttt ttatatctaa attcattttt
    29581 gatttaagac ttatactttt agaacttata gcattattat tcatacactt atgctccttt
    29641 aacttttgga tactttaaat agtatttttc gtctatactt atttttacaa ggtcaccttc
    29701 tactaaattt aaattaaccc actcttttat agaaacatca cttattatga aattttcatt
    29761 taatttatct tttatttcaa ctctcaataa attccctcta aactctatat ttgatattat
    29821 cccttggtag ttttcttttg tcgatttctc tatttgaaca tattctggtc ttacagttaa
    29881 tatagaatct ccattatcaa aacagtttgt atctcctata aattgagctg taaataggtt
    29941 ttgaggattt tggtatattt cttctggagt ccctatttgc attatatctc ctccatttag
    30001 aatagctatt ttatctgcca tagttatagc ttcttcttgg tcatgtgtta ccattattgt
    30061 cgttatatta agttcttttt gtaatctttt aatgtccatt ctaagcttgt gtctaacctt
    30121 tgcatctaat gcagacattg gttcatctaa aagtaaaaac tttggttcaa gagctaatgc
    30181 tcttgctatt gcaatcctct gttgttgtcc acctgataat gctttagggt atttatgagc
    30241 ttcattagtc aatccaactg tttcaagtac ttccttaact ttattatcta ttttttcttt
    30301 tgaaactttt ctctctttta gtggaaaagc tatattgtta taagcagtca tattcggaaa
    30361 taatgcatat gattgaaata ctataccaaa acctctcttt gatggctcta aatttgtaat
    30421 atctttatcc tggagaatta tagttccact atttacatct tcaagacctg ctattattct
    30481 aagtaatgta gttttaccac atccactagg tccgagtaaa catagaaatt ccccttcttc
    30541 aatatccaaa ctaatattat ttaaaacctt tttttggtca taacttttaa acacattatt
    30601 tatttttaag taactcatat cttatctctc cttatatagt tattttttta ttttaaattt
    30661 tatatcctac tttttcttct aaaattcttt catctaaatt attaatttaa actttactta
    30721 ctttcagatt ttgaaccgta ttttgattcc caagttttaa gtactgtact tctgtttttg
    30781 gcagcagagt ttaaatcaac ttcttttata agctgttcaa atggttgttt agaataacct
    30841 tttggaactg gattatctgt ctttatggaa gtaactgcta cttcatgtgc atatctatcc
    30901 atagctgaat cacttattgc ccaatctaaa aatagctttg cttctttttt tatattatct
    30961 tttttaacta aggcatttgc ttctaagtcc caacctgaac cttcctttgg aaatacaacc
    31021 tctataggtt ctccactaga taattgttgt gtagctctat atccaagtgt aatacctatt
    31081 ggatactctc cagagccagc taacttcgca ggttttgaac ccgagtgtgt atatgtggct
    31141 atatttttat gtaatttatc catgtaattg taagcatctt tttcacccat catttgaata
    31201 agccctgata ctgtaagata ccccgttcca gatgatgctg gatttggcat tgttataagt
    31261 cctttatatt ctggttttat taaatcttca taactttttg gaatatctat acctagtttc
    31321 ttaagttcct tataattaac tactattgct gtctctggga cactcattcc tacccaagat
    31381 acatcatctt cagtatcttt aaatttttta tcaactttat cataattttt aggcttatat
    31441 gcttcaagtg caccatcttt ttttagctct atcatgttca ttgctgataa tccccatata
    31501 atatctgctt gtggattatc tttttctgct aatagttttg aagctattac accatgagaa
    31561 tccataacta tctctacatc tatattggga tactcttctc taaaaccttc catataataa
    31621 gctatctgct cttcctctag tgctgcatat attgtaagtt tattatttcc ttttccttga
    31681 gtagtttgtg gtgttgttga tatgcaactt gtcataaatg taatcactac aatgcacaga
    31741 caagtaaata taaattcttt catatcttcc ccctttgtgt tttttccttt ttttatttgt
    31801 tttttattgt ttttgcttat gtttatataa tacacctaat ttgttaatat aaagttaagt
    31861 gtaaattaac agttcgttaa tttttagaaa tttttgttaa atatattatt tttagttaaa
    31921 tacagtctgt ttttaaacaa tatattaatt tttatagtat ctacatttta atttaaacat
    31981 aaaaaataag tctaacctct ataaagtaga atgttagact tatttttata agtatttaat
    32041 tatacaacca aatcattttt tttcttttct tcaagtgctt ttttaagcac catatcctta
    32101 accttcataa gtctagtagt acttgctctc tcattttctt caagcttcat agctatatac
    32161 ttaatagtct caatgtaatt tggtatcatt acattttcaa gagcattaac acgtctacgt
    32221 gtcttttcta tctcttgtgc aagaagctgt gcactttttt cactttctgc aagtcttaaa
    32281 agtggttgca tagcatctga gaaagcttcc atagcagaat ctaattcacc tgatgtaaaa
    32341 gcaagaccat aaggataaat atcactttga ttgttttcag ttttaaagtc aaacacagga
    32401 acatcaacac tcattatatt tctcgtagaa acatctactg aaactgattg ctttggcatc
    32461 ataagtgctg aacccagata ctcctgactc ataactgctc ttgcgattat aaagttttta
    32521 taagcagtat caagagcatt ttctgcttct tcacgcaatc gcttattttc tcttacaatc
    32581 tcaagaaatt gtttcataag ttcatcaagt ttatctttaa gaagcttatg acctcttgta
    32641 gcagttttaa ggagtttctt aagtctagtc atttccatac gtgttggatt tatatttaat
    32701 ctagccacag atatcactcc ttaggcatat atttttcaaa atattcatca cggatacgtt
    32761 taagttcact ttttggtaac atactaagaa gcttccatcc gatttctaat gtctgctcaa
    32821 tagttctatc agttctaaat ccttgtgaaa catattcttt ctcaaattca tctgcaaact
    32881 tagcatacat taaatcaatc tcagaaagtg cagattcacc aagtatagcc ataagttctt
    32941 ttgcatcctt acctcttgaa taagctgcaa aaagctggtt cattgtatct gcatggtctt
    33001 cacgagtctt acctttacca atacctttat cttttagacg agaaagtgat ggtaaaacat
    33061 ctataggagg ctgtatatct tttttataaa gttcacggct aagtataatt tgcccttctg
    33121 taatgtatcc tgtaaggtca ggaattggat gtgtcttatc atcctcaggc attgtaagta
    33181 ttggaatcat agttatagac ccttcatgcc ctttcatttt tcctgctctt tcatacatag
    33241 tagcaaggtc agtataaaga tatcctggat atccacgacg tcctggaact tcttttttag
    33301 ctgctgaaac ttcacgcaat gcctctgcat agtttgtaat atccgttatt ataacaagta
    33361 catgcatatc ttgctcgaaa gcaaggtatt ctgctgctgt aagtgccatt ctaggtgtag
    33421 atacacgctc tacagctggg tcatttgcaa ggtttacaaa cactacagaa cggtcgattg
    33481 caccagttgc tttaaaatcg cttataaaaa attcagcatc ttcaaaagtt ataccaactg
    33541 cagcaaatac aacggcgaac ttagaatcag ttccacgtac ttttgcctgt cttgcaatct
    33601 gaactgcaag attggcatgt ggcaaaccag accctgagaa tataggaagt ttttgccctc
    33661 taaccaatgt attaagtcca tctatagcag agactccagt ttgtataaac tcggccggat
    33721 aatcacgtgc tgctggattt attggagctc catttatatc aagtctttta tctggtataa
    33781 tttctggacc tccatcaata ggtctaccca ttccactaaa aacacgacct aatatatctg
    33841 gtgaaactcc aaagtcaaga ctctttccta aaaaacgaac tttactttca gcaaggttta
    33901 tacctgttga gctttcgaaa agctgaacaa gtgcagtatc tccatttacc tctagaactt
    33961 tgcaacgacg aatttctcca tttgaaagtt caatttctcc aagttcatca aattttacac
    34021 cttctacgcc gtttattaac ataagaggac cggctacctc ttgtattgac ttatattcct
    34081 ttatcatagt tcttcagccc ccctcttttt aataaggtct gcaagctcgt tgtctatttc
    34141 atcttcaatt gaatcgtaag ctgcatctat atcgccttct tctacatact tagcacgacc
    34201 aatcttttca agaactgaaa gcttgataat atcgtttata gtaacatttt gcttgatagc
    34261 atcttgtgct gacttataga attttaatat aagtcccatc attctatatt gtttatgaag
    34321 tgatgtataa gtatcaactt catggaaaga gttttgatga aggaagtctt ctcttataga
    34381 acgagtaacc tctagtatca atctatctcc atctgaaagt gaatcaaccc caaccaattg
    34441 aactatttct tgaagttcag attcaacttg taaaagtttc atagcttgag ctctttgagc
    34501 tgagaaatta tcatttacat ttttatctgc ccatacatca actttttcct gatagagtga
    34561 ataactagtc aaccagttaa ttgctgggaa atgacgttta tatgctaaag atgcatcaag
    34621 accccagaat acttttacta tacgaagcgt tgcttggctg acaggttctg atgtatcacc
    34681 accaggagga gaaactgcac caattgctgt aagtgttcct tctctttcat ccatacctag
    34741 acaatttacc tttcctgctc tctcatagaa ttgagcaaga cgtgaaccta agtatgcagg
    34801 ataaccttct tcaccaggca tctcctctaa acgaccactc atctctctaa gagcctcagc
    34861 ccatcttgat gtagagtctg ccataagtgc aacactatat cccatatctc taaaatattc
    34921 agctattgta ataccagtgt atatagaagc ttcacgtgca gcaaccggca tatctgatgt
    34981 gtttgctata agcacagttc tttgcataag tgactcgcct gttcttgggt cttttaattc
    35041 aggaaattca agaagaacat ctgtcatttc atttccacgc tcgccacagc ctatatatac
    35101 aactatatct gcatctgccc atttagcaag ctgatgctgt gtaacagttt ttccacttcc
    35161 aaaaggtccc ggtatagctg ctaccccacc ttttgtgatt gggaaaaatg tatctataac
    35221 tctttgacct gtaacaagaa gtgaatctgg tgttttcttt tctctatatg gtctttcttt
    35281 tctaactggc catttttgca tcatagttac aggtatatca ttaccttttt catctgtaat
    35341 aacacaaact gtatcttcaa cagtaaattc accagaatat atttctttta tagtaccctt
    35401 aactccataa ggaaccataa ttttacattc tactactgct gtttcttgaa cgccaccaat
    35461 tatatctcct gctacaacct tatcaccaac agatttttta ggtttaaatt cccacttagt
    35521 tgttctatca agtgaagata cttcaacacc tttagttata ttagtacctg aaatatcata
    35581 cattttttca agtggacgtt gaataccatc ataaatagtt gaaataagac ctggtccaag
    35641 ttcaacactc ataggcattc caagagaaac tacttcttcc cctggtccta gaccggaagt
    35701 ttcctcataa acctgtatag aagctttatc tccatgcatt tctataatct cgcctataag
    35761 atgtttatct gaaactctta cgacgtcaaa catattcgca tctctcatgc cctccgctac
    35821 aacaagcggt cctgacacct ttacaattgt gcctgttttc atattctctc acctgcaatc
    35881 atatacttta tcaaaattta aatattccct cctatatttt attagcacat atatttagca
    35941 ccaataaaat ttgagattgt cttttattca actttcatct attctttaaa taatatatct
    36001 gcacctatag cacgtttact agattctctt acttcattca tacctaatcc catacttcct
    36061 ccaatacctg gaataactat aatggcagga agttggtaat ctttgtattt tgctattgat
    36121 tcttttgcaa gcaatgatgc ctgttctgta atataaatta tcgcatattc atcttcaacc
    36181 aacttatgta tacttttttt aatttcgtca ctatcatagg ctgggaagat atctattcca
    36241 agagctaaaa atcccatgat gctatcttta tctccaacta ctcctacctt atacataagc
    36301 atcccttagc ctttctttta tagtatctgc gtctatatta ttaagcttac tagtcaaaat
    36361 aattctaatt gtttttatct ctgattcttt agcatataaa tatgcaatta gtggttctaa
    36421 tgtaagaggc ttaaattttg ctcctctaac atattccata agatagtcat cacaaagttt
    36481 ttcaaagaca gtaaaaccac tatccatgcc atttttacag acatcagagt aagaagtaga
    36541 tttaaaacat gatgctggag tatctgagga aaaagcttct aagaattttt ccttatcaag
    36601 tgagccacct gaaacaaaca cattcataaa catatcaaaa tctttcttca tatttttgac
    36661 tcttataaaa ctctttagat ttgttaaatc acaaactttc ataacataat ctattacaaa
    36721 tttgttttgg ctttctttag cagtttcttt catactggta aaggttgctt tatctagaac
    36781 aatatcaacc atctggccat tttgtgtctt attataaaca tcaaatgcct ctaatatagc
    36841 tgatgacatt atcttaggta aatcactaaa actacgattt gctaaagatt ctctaagctt
    36901 aataagagga attgtacctc catcaattaa atatttctca ccatctacac cagaaatttc
    36961 ttctttaatc agcactttta aattgtgata gtcattttta taaagaaata catcaacaaa
    37021 tttttcttca ggagctagac ttttaagaat ttcatatgtc tttgataatt cttgagtcaa
    37081 aatattctca aaattatgta tattattatt tgatgtatca gcataacctg cctcagctat
    37141 aattcttaaa gagtcttcag ggcttttggc ttctgccatt tgaataaaca tttgtctgtt
    37201 aagaagctgt ttttcaagca ctcttattcg tgcaacggca tagggataag ttttttcttc
    37261 tgccattttt taacccctcc ttaattaaac aaaatttctg ctgcaatttg ttcaatatat
    37321 tcatgctcta ctgcaattat ggcttcaaat gaataattgt actcaatgtc acctttttta
    37381 actatgaaac ctccagtaat atctctagtt tcatcagaca ctttaatgcc tttttttgat
    37441 agcacatctt ttaatgtttt tttatctttt ttagataata ctatctctga atcatctgta
    37501 caatttgatt tttcaatcat gtttagtata atctcttcat attcattatc tttaagattt
    37561 aaaagtttgt ttttagcttc taaaattacc tcttctaaaa tctcttgttt ttttgataat
    37621 atttgctttt tagcctccat ataagctcct gatatttcct tagaagccgc tttttctgct
    37681 tctgcctctg caagctttgt ataggaagcc atttcctttt cagctttttc attagctgaa
    37741 tttactttta aatctgcttc acttttagct ttatccaata tttcttgtgc ttcttgtttg
    37801 gcatcagcta taattctgtc aatcattttt tgttcattac ccataggagt gcttcctcct
    37861 tttcattttt tttacttatg aattataatt tataattaac caattgcaat tccattgtaa
    37921 gctaagaaag aaacaagtag tcctaaaaga gcatatgttt caactattgc tgcaaatgtt
    37981 atagctttac caagttcttc aggtcttttt gcaactattc caactcctgc tgctgctgct
    38041 ttaccttgtg ctatacctga tactaaacca actaaaccaa ttggaagtcc tgccataaga
    38101 agttgtaaac cttgagctga tgttggtatt gcatctccac caataatccc tgcttttgat
    38161 aaaataagga aagctatgat aaatccatac agaccttgtg taccaggaag aagttgtaaa
    38221 actagaagtt gaccgaattt actagggtct tctgttacaa cacctgctgc ggcttgacct
    38281 gctattgaag tacctttagc agaacccata cctgcaaata atgctgctat agcagctcct
    38341 gatattgcca aaaattgacc atttcctaac gcgtttaaaa attccatatt tacatctcct
    38401 ttttattaat gcttgtatat tttgttttat acttaaacgg aacaaaagga actcctccgc
    38461 cttcataaaa cttattgaaa aattctacat actgtaacct acttgtatga acatatgcac
    38521 ctagtgcatt tataagaagg tttattgtgt gtcctacaac accaattaca actgcaagta
    38581 tcggaccagc tatagcccct aaaaggttga taacttgtgc tataactcct gttgttaaac
    38641 aaagcgccat aattcttgta taagataata tatcagcaaa ataacttgtt ataccataga
    38701 gacttgaaaa tcctttaaaa agcttaacag gaataccttt aaagcttctt ccttgagtaa
    38761 gtacaagacc tatagcacct ataattgcta aatattttcc tacttctgaa aatacaccta
    38821 tgtcccctgc aacaaaaggt attataagta gacatacacc tgttatacac aaataccaaa
    38881 aacctatgtc aaagattgca tctattacct ttccacttct tattaatgta tatgctttta
    38941 ttcccaaacc tacatatata tggataatac cgaacagtaa cgacattccc ataagtgtca
    39001 taacatcctc taaaggatta attaatgctt ttataggaat taaatccccc aaaatacccc
    39061 cgaatataag accccataca gtagttgaaa ctccacagat accaataagt ttaattaaat
    39121 ttccttcacc tttttgtatc tttaatttat atacaacaaa tccacaaact actgcaatta
    39181 ttaacccata tgcggcgtca cttagcatca ttccaaagaa tataacaaag aagaaagtta
    39241 aaattgtatt tgggtcaata tcttttgtac ttggatagct gtacatgtta gttacacttt
    39301 caaaaggtgt tactaattta ttgttttcaa gcagaattgg gaagccttct tctttacttc
    39361 cttcttcagt ctgaacacaa caatcaaact tttctgtaat ttctttaatt aattcatctg
    39421 cccttttgct tggtaaccaa ccattaagac agaatgttgt ttttgttttg acaagtctct
    39481 ctataatctt tttttggtct ctttctatag tgtaaaaatc atataggttt tcaagcatgt
    39541 gtatactatc tgcatgtctt ttaatttcct ctgttttgtc cttagaaacg ttttcctgtc
    39601 taattatcat ttcattgtat ttcttgatag cttgtaacgg tgtaccttct tcatttggta
    39661 atgaaaccac tgaaaaatta aattctttta aaacttctaa aacatcatca aaagtttctt
    39721 ttaaagtaat taaataaaca tagttcatta acttgtcaga attgacaatc ccaataacac
    39781 tttctggtat ttcatcttct aattttactc ttaaagcatc aatgtttgcg cttatgggca
    39841 cagttccaag tatagttttt gtatacttag tttccatatt ttcaatagga atatcaaagt
    39901 tcacccatgg ttctaacata cttttattgt tgttaagtaa gtttatgtta gaccttattg
    39961 tacctatctc cttgtgcaaa ttgtttaact ccagagcttc agcataaact tgcccttctt
    40021 tttcactatc aattttgtta aactctcttt ttggtgcaag tagggatttt ttttgtttca
    40081 ctagtgaaga aacaatatta attgcttggt ttacatgaga cattttctgc tctagcatta
    40141 taacttcaga ttcttcacta tcttttacaa gataattttt taattcctcc tcctcagtaa
    40201 gaaaggcact atcatcaatc tgaacaaacc cacggttcat aagtagcttt aaaatctggc
    40261 ttttttgagc ttctaagcca acaatagata ttttgttcat attaactatt gccatattat
    40321 ctcactacct ttccaataac cgcttcaata gccttacttt ttctttcttc agcagttttt
    40381 tcaattttca gacattcact tttagcatta gctaagattt tatcaacctc tgctctactg
    40441 tcggtgactg ccttctcaat aagagtttct tccataagct ttgcttgttt ttttacatca
    40501 gcaaatatgc tttcacattt gtttacagca tcagctctga tagactctgc atcagtaaca
    40561 gcttttactt tgatactgtc tgctgtctgc tctgcctcaa caatgctttt tattatttca
    40621 aaagccatcc aaatcacctc tcttaccttt ttactatgta tataaccaat tagatttttt
    40681 tattctaatt agattactac ctcattgtta aagtataaac agcattttta tataataatt
    40741 aatatttttc aacgtttatt tcttaataaa acatatttta gaatagtact tgcctagatt
    40801 tacatccttt tttgacttac ttttagac
//
LOCUS       DACULQ010000014        38942 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.014, whole genome shotgun sequence.
ACCESSION   DACULQ010000014 DACULQ010000000
VERSION     DACULQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.014"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..479)
                     /locus_tag="JA154_03170"
     CDS             complement(<1..479)
                     /locus_tag="JA154_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HAT7994858.1"
                     /translation="MFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPIYSNN
                     TVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRGGKCE
                     ELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVSSYIS
                     "
     gene            complement(702..2111)
                     /locus_tag="JA154_03175"
     CDS             complement(702..2111)
                     /locus_tag="JA154_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994859.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            complement(2115..3245)
                     /locus_tag="JA154_03180"
     CDS             complement(2115..3245)
                     /locus_tag="JA154_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAT7994860.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            complement(3257..4060)
                     /locus_tag="JA154_03185"
     CDS             complement(3257..4060)
                     /locus_tag="JA154_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994861.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            complement(4442..5323)
                     /locus_tag="JA154_03190"
     CDS             complement(4442..5323)
                     /locus_tag="JA154_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7994862.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            5779..6753
                     /locus_tag="JA154_03195"
     CDS             5779..6753
                     /locus_tag="JA154_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994863.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQILRDNNFYK"
     gene            complement(6869..7930)
                     /locus_tag="JA154_03200"
     CDS             complement(6869..7930)
                     /locus_tag="JA154_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAT7994864.1"
                     /translation="MKKIRKLTTKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(8542..9393)
                     /locus_tag="JA154_03205"
     CDS             complement(8542..9393)
                     /locus_tag="JA154_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT7994865.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(9410..10330)
                     /locus_tag="JA154_03210"
     CDS             complement(9410..10330)
                     /locus_tag="JA154_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HAT7994866.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(10440..11354)
                     /gene="murQ"
                     /locus_tag="JA154_03215"
                     /gene_synonym="yfeU"
     CDS             complement(10440..11354)
                     /gene="murQ"
                     /locus_tag="JA154_03215"
                     /gene_synonym="yfeU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089561.1"
                     /note="catalyzes the cleavage of the lactyl ether moiety
                     of N-acetylmuramic acid-6-phosphate (MurNAc-6-P) to form
                     N-acetylglucosamine-6-phosphate (GlcNAc-6-P) and lactate;
                     involved in MurNAc dissimilation pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAT7994867.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(11453..12550)
                     /locus_tag="JA154_03220"
     CDS             complement(11453..12550)
                     /locus_tag="JA154_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAT7994868.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKRINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDIGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(12783..14129)
                     /locus_tag="JA154_03225"
     CDS             complement(12783..14129)
                     /locus_tag="JA154_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT7994869.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPELLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(14218..14526)
                     /locus_tag="JA154_03230"
     CDS             complement(14218..14526)
                     /locus_tag="JA154_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT7994870.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(14788..15096)
                     /locus_tag="JA154_03235"
     CDS             complement(14788..15096)
                     /locus_tag="JA154_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994871.1"
                     /translation="MEKNQRTDYNIPTISFKSGLILIVSSLVSTVVFPLLLSIFGIDN
                     RFGVVIGNSLITSFAVAYTRYFVETKRGFSLGFFRLYILFALSFAIIGYFWIYKGIHI
                     "
     gene            complement(15188..16339)
                     /locus_tag="JA154_03240"
     CDS             complement(15188..16339)
                     /locus_tag="JA154_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAT7994872.1"
                     /translation="MYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEIPKD
                     IKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHGQTI
                     FHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEFLLY
                     SDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYDKNG
                     YFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDLIAT
                     VTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQDEYG
                     YSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(16657..16743)
                     /locus_tag="JA154_03245"
                     /pseudo
     CDS             complement(16657..16743)
                     /locus_tag="JA154_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
     gene            complement(16779..17798)
                     /locus_tag="JA154_03250"
     CDS             complement(16779..17798)
                     /locus_tag="JA154_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7994873.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSIDS"
     gene            complement(17792..18472)
                     /locus_tag="JA154_03255"
     CDS             complement(17792..18472)
                     /locus_tag="JA154_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7994874.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(18624..20531)
                     /locus_tag="JA154_03260"
     CDS             complement(18624..20531)
                     /locus_tag="JA154_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7994875.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(20532..21284)
                     /locus_tag="JA154_03265"
     CDS             complement(20532..21284)
                     /locus_tag="JA154_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacitracin ABC transporter ATP-binding protein"
                     /protein_id="HAT7994876.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(21600..22760)
                     /locus_tag="JA154_03270"
     CDS             complement(21600..22760)
                     /locus_tag="JA154_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7994877.1"
                     /translation="MIMINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFER
                     GIILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYL
                     LLVALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFS
                     YFGLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLL
                     YSMVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKL
                     SAIMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLI
                     ALSIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(22757..23233)
                     /locus_tag="JA154_03275"
     CDS             complement(22757..23233)
                     /locus_tag="JA154_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAT7994878.1"
                     /translation="MSIMFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFA
                     MIAKDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQ
                     GQEVNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(23238..23726)
                     /locus_tag="JA154_03280"
     CDS             complement(23238..23726)
                     /locus_tag="JA154_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994879.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(23740..25071)
                     /locus_tag="JA154_03285"
     CDS             complement(23740..25071)
                     /locus_tag="JA154_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HAT7994880.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(25085..26650)
                     /locus_tag="JA154_03290"
     CDS             complement(25085..26650)
                     /locus_tag="JA154_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS alpha-glucoside transporter subunit IIBC"
                     /protein_id="HAT7994881.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(26820..27605)
                     /locus_tag="JA154_03295"
     CDS             complement(26820..27605)
                     /locus_tag="JA154_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiR family transcriptional regulator"
                     /protein_id="HAT7994882.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(27772..28986)
                     /locus_tag="JA154_03300"
     CDS             complement(27772..28986)
                     /locus_tag="JA154_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HAT7994883.1"
                     /translation="MDENYMKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFN
                     YVYNVVKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEEMSSILSDLNNK
                     HTEVIDNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILK
                     DVIKGQIGYMYLPSMSSKNGSINNDLKIIGDYIDTLEKHKALIIDIRGNLGGSDSYWQ
                     GIVSKLIKNDVKINGYRIYRNNNEIVKQYTNARNIKLNPVENLPTSVKENAPKEIIKD
                     FNDFEDTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGD
                     GGCIDPVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNC
                     IRKALELENINY"
     gene            complement(29493..30830)
                     /locus_tag="JA154_03305"
     CDS             complement(29493..30830)
                     /locus_tag="JA154_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAT7994884.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(30926..32470)
                     /locus_tag="JA154_03310"
     CDS             complement(30926..32470)
                     /locus_tag="JA154_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAT7994885.1"
                     /translation="MEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGWAE
                     QDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIWCD
                     QRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLPKD
                     YIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITGYV
                     TRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPKID
                     KEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIINNQ
                     VSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYSLK
                     DCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAILAL
                     VGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDEVV
                     NAKINN"
     gene            32684..33844
                     /locus_tag="JA154_03315"
     CDS             32684..33844
                     /locus_tag="JA154_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7994886.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(34000..34404)
                     /locus_tag="JA154_03320"
     CDS             complement(34000..34404)
                     /locus_tag="JA154_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAT7994887.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(34425..34907)
                     /locus_tag="JA154_03325"
     CDS             complement(34425..34907)
                     /locus_tag="JA154_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIB"
                     /protein_id="HAT7994888.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(34927..35736)
                     /locus_tag="JA154_03330"
     CDS             complement(34927..35736)
                     /locus_tag="JA154_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAT7994889.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(35729..36508)
                     /locus_tag="JA154_03335"
     CDS             complement(35729..36508)
                     /locus_tag="JA154_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT7994890.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(36508..38586)
                     /locus_tag="JA154_03340"
     CDS             complement(36508..38586)
                     /locus_tag="JA154_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAT7994891.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
BASE COUNT        12749 a         6870 c         4088 g        15235 t
ORIGIN      
        1 gaaatatatg aactaacttc attaggagaa atatctaaaa tagttgcaaa ttcttcattt
       61 aataaccttt cagcttcttt cattatatta tcatctgctt gatgtgtttt tttaccaata
      121 gattttacat actctctgtt attatctata cttcttacta attttattaa ctcttcacat
      181 ttaccacctc tgagtatttt tttaaattgc tcacttctca atttttcatt atctatccat
      241 gatgtatcca tattaggaat atcatttatt aatgaaagca cattaatttt agaaagtaac
      301 tttctcattg gtactttttc attatctaca ggaattttta ttactgtatt attagaataa
      361 ataggactca atacataata ctcttttttt attccatttg taaatcgttc atttgttata
      421 tccatgactt tgcagactcc tgtcatacca tacattatat aatcatcaat cttaaacaaa
      481 aacatcacca tccttaataa aaaaaatagt tttgacaact aaatccacct tgccaaaact
      541 atctgctata tattatattt taacacattt taagacttaa atgtaagtaa aacttatatt
      601 attaaaatta aaaaatacat taaccattga ttaatgtatt ttttagattt ttataacaag
      661 catatcaaaa taaattaata cagcttatct cctgtgaata cctataaaat tggtgcttgg
      721 ctattcattg agtacccatc atttcttaca tcatttattt ctaatatttc atcatttgtt
      781 ttaaccttag taagtttttt aactgctcca taaggttctt ttaatgaaat tttatctcct
      841 cttccagaaa taacccataa aattttatac cctctaggta ttacttgtag tttattttca
      901 cctttaccat cagtaaagta gactaacaaa ttaatctttt tattattagc atactcaaag
      961 actggagaaa atctagtacc tccacctatc ctaattctat cttttatatc tttttcagtt
     1021 ctcactttat atacacgtct aatttcatta tcacattcta taattgtaat ttcatgatta
     1081 tagtttttca ctatactaag aacttcttta atagcttgtt taaactcttc atcactaata
     1141 cttccactta catcaagtgc aactgctatt tctgacttat gacttctaag ttctcccctc
     1201 aaatctaaac gatttggttg tcttctattt cttcttgtta tagtcttctt tttattattt
     1261 tcaactgttc ccattattct attaagatat aaattccatg gcaattcacc cttactattt
     1321 ttaagtgatg atatcatact ttctaaataa gcaggaactt tacctttttg agaactgtta
     1381 ataaattttt cagtaaattc tctaagggta ttttcatcta tctcattgga ttcatcccat
     1441 atatcatgag ttctttcggg gctataatca atttctatat ctccatattt atcactatca
     1501 tcttcctcac catcttcatc cacttccatc aaatctaatt cagtctgaat cttttctaca
     1561 taatattcaa aaggttcata tggttcaagt tttaaggaat actttaaatt tacccactct
     1621 aaggtagttg catatggtgg taaattgttt agaaatttat ttactactat atccattgcc
     1681 atattaattg ctaatgtact atactttcct tttaactcct ttgctctcat caaatgcata
     1741 gataatatat gatgaatttc atgtttaatt gtagtctcca tttgctttat gtcaagcgtt
     1801 aaaaagatta ttggattaaa atatataaca tatttagctc ccttaaaatt cacacctgta
     1861 ggactactta tatcaaatct tatttctctt gacatctgaa acaaaaaata tccataaaaa
     1921 ttatcttctt cttccataag acttaaattc actttatcta caagactaaa aaactcatac
     1981 ttaaaatctt gtggtatatc tatatcaaat ttctcattag cacgatttgc ctttagcata
     2041 gaatgagtat ttataatttc acttgcttta ttataaagct cttttgcttg tttatcaaaa
     2101 taattttcca tacattatcc ccttattaaa ctataagatt caaaatatga ttttacaaac
     2161 tcttcatttt ctatagcttt tttatatact tcaatataac tgtttctaat atccttcata
     2221 atacctatca ttaaatctac aggatacatt tttaaaaatt caataaatct attaatatag
     2281 tggtttgatt catatttatc attcttaata tttaattcta agtcttttag aatgttcata
     2341 gctgatagat aaagtcttgt atgactctca ttttttactc tttctacaat agactcatca
     2401 atagattctc ctaaaaaaac atcttcatag gatattaatg gttcagagtt tgattcaata
     2461 aaacttataa attcttctgc aataacttta cctacattac cctttacaac atttaaaaat
     2521 actgctctag gaatagaatt atttttttct ttataaactt tataaatttt agatactctc
     2581 tcataacttc taggagtggc tcttacatca tcttcattta ttttatgcag atattctgga
     2641 aaagtggaaa taaactcgat aacttttctt tcaataccct catctattgc ccattttatc
     2701 cattgtgtat ggtcactctc catatttaac caaacaaacc tattttcttg ggctgcatcc
     2761 atatctacca cttgataatc aaaatctgaa ccatatttac ttgatggatt cattgctgct
     2821 aaaatcttta catcatcatg cagtttatat ccattaattt ctctatttaa aattaaattc
     2881 ataagttctt gctgtactgt atgctcacaa cgatttatct catctataaa taaaagaact
     2941 gtcttacttt tagatatctc ttcatcaatt tctcttaatt tgttatgaac agcatacact
     3001 gttgttttct tttcagtctt atatccatta gaattaactc ctacataaga ttctatagtt
     3061 ggaagaccac ctatttcacc ctctttaagt aagtttccat caataacaat taaactccaa
     3121 tcattttctt tggcaagctt atttgctaaa gctgtttttc ctattccact ttcacctact
     3181 attaaaggta cttctccagt ggataatatt aagtcaacgc tttttaatgt gtctataaaa
     3241 ttcatttttc tctctcctac ataatcttta atttctcatc tacagctatt ttttttgctg
     3301 tcttacttcc atatttataa ataaacataa ttttatctat tggtatactt tcgctatttc
     3361 tatctataac actacaattc aaaaaatctc taatatactt ttcctcaaca tcttctttgg
     3421 ataatacttc ttttaataca ctctctaatt catctttaga tacttttttc agaatgtact
     3481 taatcactaa aatattcact tttaaaaatt ccaacatttt atttttatct aaatcagtta
     3541 taaagctaat agctgcttca ttatttttta tagctataag ttctgcatta taactaggtg
     3601 aatctatata tctcaaggca tcataactta ttctcactgc ctcctcttgt acactttcat
     3661 aaggttgttt tataaacttt atcgaatcat aattttttct tactgctaac agttgtaatt
     3721 cttcacttgg atttttagca tatttaattg cccatcctgc ttgatttata gccatttcaa
     3781 taactttatc agttgggttt ttgatataat ccagtatact ccatccctca tttactgctt
     3841 ttatcatcat atcttctgtt ggattatcta taaatttaat tgctctaata ttattatcta
     3901 ttgctagttc ttgcatctct ctggttggat tatttatata ttctagtaca agcccatttt
     3961 ctttaatagc taacaatttc atttcatcag ttggattatc tattgtagct ataatattgg
     4021 gattattttt tatcatttca ataactctca atttttccat aatgtgcctt tctttctata
     4081 attattttta tatctatatt aattataata atctattcct atttatataa ttgtttctta
     4141 ttataattaa tatacttttt aatcaaattt aaacagaata attataccaa tatataaaaa
     4201 aatttctaaa aaacaataaa attaagttat aaacaccaat atatttctat tatttcaata
     4261 cgtatacaat tagattagaa tctaacgcta gtaataaaaa attactttcc caaagtagat
     4321 ataagcctca ttttatcgtt caaataatga aaatatattt gatttcatgc aaaaagagtg
     4381 cctaatgaac taaaaagttc attttgagac actcattttt ttatataagt ttatatagtt
     4441 tttaatcgtt cgcattcaac cactcagtta agacttttat atatttctca ttttcttcaa
     4501 cgaatggcat atgtcttgaa tactcaaata attcccactt actattaggt attctatcat
     4561 acatagtctt tgcaatatat ggtgaacata aatctattgc tccactagtt actagacaag
     4621 gctcttttat ttcatgtaat ctatctgtaa attcatatcc agaaagagtt cctgtaggag
     4681 aaaattcatt ttgtccccat cctacaatat atgcttcaga acctgatttt ttaggtcttc
     4741 tcaggcactc tggagaatct tctgtcactt cacctgcaca atacatttcc ataaatcttt
     4801 ctaatgcatc attatattct ttgctagagt aatctccagt atttactgca tcaagtaatg
     4861 ctttttggtc aacctcactc atatatgata ttcttctttt ttgttctttc tcccaaagtt
     4921 ttgcagaaga aagagtagat gaaaggatgt aagacttaat accttttggt ttatattcaa
     4981 tagcatacca aatagcctgc atacctcccc aagattgacc taacagatga atttcatcaa
     5041 gtcctagatg ttttcttagt tcaataagct cctctatcca agtatctgca ttaaatagct
     5101 caggatgccc ttctacaaat gaatttccac aaccaatttg gtcatacatt attacttgtc
     5161 taccactttc agctatctta tccaatacct caaaatagtt gtgtgtagaa cctggacctc
     5221 catgaagtaa tactaatggc ttcttccctt ccgtattttc tcctactata cggtaatacg
     5281 ttttaaaacc tttaaaaggc atatatcctt cagtaatttt catgtttatc tcctccaatt
     5341 tgtaattata aacaataatc aagttcatta acaatactat ttaaaacctc ttatacatat
     5401 ttgattatgt attttaatct ttatacctac ttttataaat agtataacat aaaagttaaa
     5461 aatctctata ctaaaccaat tataaaatta tattaactat ataattttat tttttaataa
     5521 tagtatatac atatttgatt attttcattt gctatttaaa atcaatttta agttttcttc
     5581 ttataataaa cttcctcttt gtggtctcat gttagtctct aaatttaaaa ttttacaaaa
     5641 aattttcata aaataaaatt atattgattg tatctaaaca aatagggcaa taaaataatt
     5701 aagtatagtt acttaccttt caagcaatat tagttttatg ttagaatttt attatattga
     5761 tttacaggag gtttgcattt gaataatatt aattcactta aagtaataaa attatctctt
     5821 ggatgttctc ttgctatatt tattgcttgg ctattaaaat tagaatattc aatggttgct
     5881 ggagttatag tactacttac agtaaaggac actaaaagag aaactcttaa aggctccatt
     5941 ggaaagattt atggttttct tttatgtact attttttcat atttatgttt taatatttta
     6001 ggatataatt tgacttcatt ttcaatattt atatttataa ttataccact ttgttttttg
     6061 ttaaatattc aagatgttat tgctatgtgt gtagtcatag catcccatta ctaccttcaa
     6121 ggagaaactt ctatcaaatg gattttaaat gaagctggaa tatttgcagt aggaactgga
     6181 ataggtgtct taataaatat gtatattcca acaaatatac ataaaatcca tgaagttcaa
     6241 aaaaagttac aggaagaagt gagtattgta cttatagaca ttgcagatat tattgtaaat
     6301 cctaaaaaag aaaatggata tagtagagat ttatatacat taaattcact tattgacagc
     6361 agtatttctg aaacttacga taatattaat aatactttat taagtgatac tagattcttc
     6421 ttagaacata tggatattat aaaaagccaa cgggatatct tagaaaattt gtatagttat
     6481 gtatctcaac taaattctac accaccacaa gctcatattc tatctgcttt tatacataag
     6541 ataggttata ctgaatttga agctgaaaca ggcaatcttc ttcttgaaga attaaagaga
     6601 ctaatgctta gtatgaaaaa ccagcctctg cctgttgaca gaacagagtt tgaaaataga
     6661 gctattttat tcctatgctt gactgagtta aaacagtttt tagttaatcg taaacatgct
     6721 cagatactta gagataataa tttttataaa taaaaagggg agaaatctag cagttgacct
     6781 gactacaaaa tatcagatat aatattaaat gcgcccctag taagagggcg caatgtttat
     6841 aacttattct actgtcatga ttaaatcatt attcatatcg aacatacatt atttgaccaa
     6901 gtccaatatt agctttttca tcagtgattt ctattttaat agtcacatca ttttttttac
     6961 agtacttcgc taattctgca ccagaacgat ttttaccact aaaatctaaa gaaaatggct
     7021 tatcatattt aaaaggatat tcaacaggtt taccctctgg tgctaatgca aacatcccat
     7081 atggtgtgtt cataaatcct attacagcaa tcgtaagatt tgatgggtct aaatttgatt
     7141 cactacctgt aaatgtaatt gtatttactg atgaattaac tacactacat ctaacacctt
     7201 catacattac atgtttttca tttatatctt ctcctttttt tccaaaattt ccaacagcct
     7261 ttgccccttc ttgatgtggt aacatagtgt gctttccagg tggtgctggt ttctgtgtct
     7321 gttctggttt taccgtctgt actattgcac caacttttgg atatgctgtc ttatcaaatg
     7381 gtaatgggtg gctaagactc ttgtatatga tatcctttcc tgctgattta ttagcatcaa
     7441 tatctacatg atacaaacgc ccatcaccaa aacggataag attccactgg tggtcatttg
     7501 atgtgctgtg tacataataa gattcaattc ccatagcatc acacaaagct ttcatgctcc
     7561 tagccattcc tgagcatgca ccttttttag taaccaatgt ataggcagta tttgctccct
     7621 ctactgcatt ccagtcaaca tatgttgtgt tatttttaat gtgattgtag gcaactaaaa
     7681 tcttagcatc tgtggtcata ttctttgtga ttttcttgtt cataaaatct gctgttgctt
     7741 gtgctactga gtttagttgg gcttgtgaca gctttggacc atatacgcta ttggttccag
     7801 tgacatagct ttttggtgtt tttgctgtta cctctttggc tgtcatacca taaactgtca
     7861 tactcatacc tgagattaaa actattgttg taatcaataa cttggtagtt aacttcctaa
     7921 ttttcttcat acttaatatt ctcctttata aaacattgtt acttcctatt ctctaccatt
     7981 aataaaatta atttagtatt taaaaaggtg gattgtcaac atgtatattt ctccattcat
     8041 acattcgtga aatatttaaa ttttatggaa aatctaatag atattttcta gataatttgt
     8101 atacaataca actcgcaaat cagggtattt gctgttagtt tgagaaaatg atgatgatta
     8161 atacattcgg aatctttgac ctataataaa taccattgtc ttcaatcatt aataatggta
     8221 agagtaaaga gaattatcaa taatacagtt aaaatcaact ttgtatacat ttcatacatt
     8281 ctctcccaac aacattatac ttcttataat ttgtatattc aatacgtttt ctgggaaaat
     8341 ataggatata tctataaaaa tataaaaaag gagttgaact taaaatttga ttaaaaaaaa
     8401 tcaattttaa gacaactcat ttgtattata aaaatgctat atattattat taagtaagtc
     8461 aatattttag atatttatct atttcttatt gacttacgct tatagcctaa cttatttgtt
     8521 tagttgatat ataacctagt tttaagcatt attaatattt aaataatcaa gtatcctttt
     8581 tgtttcttgt agtttctgtt ctgcctcatt taagttttct tgaattatag aaatatatat
     8641 tatatctatc aacgtaagca ttgctattct tgaagaaata gctccttctc taagtctttt
     8701 ttctatattt ggtactacta gctttaaatc actaatctta ctaagatgat tgtcactata
     8761 tttagttata caaatgacct tacatccttt taattttgca ttttctatag actttattac
     8821 ttcttttgat tctccagagt aagatattgc tatagctata tcttctttat ctacatttga
     8881 agataccata agttgtgtat gactatctaa agaggtaaat gcctgtttat ttatccttaa
     8941 aagtttcatc tgtaaatcta gtgcaactaa agccgaacca cctactccaa atatatatac
     9001 atttttagcc tttattatct gttcaactgc ttcttttatt atatcttttt cattcaacaa
     9061 ccttgtttgg ttgatagtct ctatgttttt gtttgtaatt ttgtttataa cactatctat
     9121 attacaatca tagttttcat ctacatatgt gtattcattt tctttgccca tcaaatcttt
     9181 agctatctca atttttaaat ccccaaaacc cgaatatccc atttttctag aaaacctaac
     9241 tatacttgct gtacttgttt ttgagtttga cgccaattct gatacagaaa gcttacttat
     9301 agcctctgta ttattaagta tgtaatcagc tattttttta tctacttccg ttaaatcttc
     9361 atatatctgt tttaatttta aaatgcaact catactctat cacctctacc tatttactat
     9421 aatatttctt cttagcaagt aaacatgctc ctaaatctgg agtcaacact ggtctcacta
     9481 taccaaatct cgttttattc aaatattttt taagtggctc taatattaaa tctcctgact
     9541 taaacactcc acctgaataa gataccttta tctttgtacc ttgttcaaaa tgatgctcta
     9601 atcctattat aagcctactt aattcataag ctgcatcatc aaatatttta ctagcagtat
     9661 tatcaccttc aataactgct tttaaacata tcgtagcaaa ctttgctatt tctattctat
     9721 ctccttgtat ttcatcattt acatatttta ttatgtcata atcattctct atattaagat
     9781 gtctttttat taaatcatat aattttgttt ttgaacacct tccatcagac tgcattgtga
     9841 acatttttaa ggtagccatt cctatataat acgctgaacc ttcatctcct aatacatagc
     9901 cccatcctcc acatctgtga agattcccat ctttatctaa agcctgtgct attgaaccag
     9961 tacctgcaat tatatttata cctttttccc catttaaaga acctgctaat gctatttcta
    10021 catcattacc tagagtataa tttatatgac tatatgcatt ttttgttgct acttctaatg
    10081 catacaatac ttcttttatt tttccatatc cagcaagtcc aacaaatgca tacgttattt
    10141 cttctacatt tatcttagca tctttacaaa cttcttccaa cccagttatt aataatttcg
    10201 ttagattatc aaatcctatt tgattataat gacaagttcc tttggttatg gttcctacta
    10261 tatttaattc ttcatctact aatgtaaatg ttgttttcgt tcctccacca tctacaccta
    10321 aaaaatacat actatcctcc tacaatatct ttaataattc tttcaatact taatatctaa
    10381 aaataacctt ccaatgctgt ttttttaata ttgaaaggtt atcagtacta ttatttattt
    10441 tacaattttt ctattgattt taaagcttca cttatataac cttcttgtct atctaggata
    10501 ctctttgcac tatccttatc taaaccactt tttatcataa atatagccaa tttaacgcta
    10561 ttatctgctt cttctaagaa tttttctgca acatcatatt ttacatttgt agctatcatt
    10621 attattcttt ttgctctttc aaccaacttt tcatttgatg tcttaacatc taccatcaag
    10681 tttgagtata cttttcccat cttaaccata gaagctgttg ttaacatatt acataccatt
    10741 ttttgagctg taccactttt cattctagtt gaacccatta ttacttctgc ccctactata
    10801 atgtttattg gtaaatccgc ttttttactc atttcactat tcggattcat agttattgat
    10861 agtactgcac accccaattc cttagcatac tccattgcac ctataacata aggagtcctt
    10921 cctgatgcag ctataccaca tataacatcc ttagatgtaa ctcctctatc tattatatct
    10981 tgctttccaa gttctttatc atcttcagca ccttctattg ctttaaatat tgcttgattt
    11041 ccaccagcta ttatagcttg aacaagctca aaatccactc cgtaagttgg aggacattct
    11101 gtggcatcta gtattcctat cctaccactg gtgcctgctc ctatatagaa taatctacct
    11161 ccctgcttca ttctatctac tattatatta acagcttttg ctatactctc cttttgttct
    11221 tcaacagcat aggctacagt tttatcttcg ttatttatta attctattat gcctaatgta
    11281 tcttctttat ctatttgcaa tgtattctta tttctacctt ctgtaactaa attttctaaa
    11341 gtttttatta acatatcatt tcctcatttt cttaatatta aatttttaaa cacattttaa
    11401 ttccaacttt cataattata tttttataat aaattaatat tttcataata aattattttg
    11461 ttgagggttt aaaattaaac ttttgccatg gtttaatata atctaatata aataattctt
    11521 cttttcttat atatcctact acattcgatt taccactgtt tttcatatca gtcaaggcta
    11581 tttgtaactc ccctgcataa tgaccatatt cactactttc aattactata tctcctcttt
    11641 ttattatttt aggagtatta aatagttcaa atttgtatcc tttatattta acacgagatt
    11701 gagtagacct tatcagactg tcagatatgt cccctctatt aaaatgtatt tcttcaaaga
    11761 gtatcttctt ctcaacttct cgtatatttt cctctattgc tacatcaaat gtaaccatat
    11821 cttttctcat agaacctaat acctttagct cctcatatgt tggataacaa tttgatatta
    11881 ttatatcatt tatattattc atagctatta tatgctttac ttgaatatct ataggtagat
    11941 ttctgtgcat ctcaattgtt ggaagcccat cagttacagg ccatggtcca aatgtagttt
    12001 cattttgact tgttacaaaa actgctgttc ttaatccata cttattaaat ctttctgtac
    12061 attttataaa gtgttctaaa tttaatccac tatatctatg tggataaaag ttatgacatc
    12121 ctgtcaagtt ttctttattt ggttggtagt ccattattgt atctatataa ttagtattat
    12181 tactcatatt gatttctatt ttaagatttt gaggattaaa agtcattaat gcctcttcag
    12241 aacctgtaaa tcctatatca agccttattc cgtctgcctt cgtcttttta aaaaaagtca
    12301 aatctttgta gcttatttct aattcaccaa atactctggg atttacatca agtattacct
    12361 caaaaccttt aaacttagcg tattcattta tacgtttaaa ttttttaatt atattttctt
    12421 tagtatcttt aactgataat aaacatgaaa atatacgtgt aaatccattt tcagcagctt
    12481 tatctatata atcatatatt tccttagatg ttgttttttc tggataaatt gatattccta
    12541 atcttcccat ctgtgtcatc tccttatatc ctatatgtgt ttaaatttat ttcgaattac
    12601 acgaaacttt tttaaaaatt tggtgaaaaa aagtttcata tatatttata aaaaaagaaa
    12661 agtttacctt ttcttttttt ataaacaatc aacaaaatat cttcgttact tatttcataa
    12721 ctgttatact ttgaaattta tactaatcta atatagtttt taatttccaa tatagtttct
    12781 aattaataag cttcttgctc ttcaactttt tcttcatgaa gcaatctctt atcgtacatc
    12841 ttgacaaatg gatagtaaat tattattgat atagctatac ataataagtt aagaactgaa
    12901 gctctccaat ctccaccagt ggctaaatat gcccctactg gtcctggtaa tgtccaagga
    12961 gcagtaacta ctactctgga tactaaatta gcactcatag caaaccatga aactgttgcc
    13021 attactattg gtgctacaac aaatggtatt attaatattg ggtttaatac tattggtgct
    13081 ccaaatatta ttggttcatt tatattgaat attgctggta ttacagttgc ttttcctaaa
    13141 gactttccat aagctgattt tgctctaaat gcaagaagta aagctaagcc tattgtagct
    13201 ccagaacctc caatccatat aaaccattga aaaaagggtt cagcagcaat acctggaact
    13261 gcttgacccg cagcatatga agctgtatta gcatctaata attgtatcca taaaggtctt
    13321 gccaatgaac ctattattga tacaccatgt attccaaatg accaaaaaaa cgttattaaa
    13381 aatattagta taattactga tggtaatgtg tcagtggctg aaactaatgg agttactaat
    13441 ttagctatga agccatgcca atcaaaccct aggaaataag taacaatagc cattaaaatt
    13501 attattaatg cggctggtgt cagagcttca aaagaccttg tgactgatgc tggaactgct
    13561 tctggcatgg ttatcttgaa tttagatttg tcagtaaatc tataaatctc aacacctatt
    13621 atagatgtta caatagcaac aaacattgaa cctccaccaa gattagccat tggcatttgc
    13681 catccttgag gaattgaatt ataccaagct aaaagttctg gactcttttc aactatcttt
    13741 aataactcag gggtaagacc ctctattgca gtaggaacta ttgtcaaaag gaatgtagct
    13801 gtagctaata aacctcctgt caatccatct aacttatatg actttgccaa actataacct
    13861 atgccaaatg tagaatatac tgatataata aaaaatgtca ttctatatgg tagtaatatt
    13921 gtaactgcat tttcctttag atactgagtt accccccatg aatctggtaa tggaggaaat
    13981 gctaatatca agaaaaatga cccaactatt ataagtggta atgaagctac gattccatct
    14041 cttacagctc ttaagtgacg ttgttctgcc aatctggcca tagaagttga taatttagtg
    14101 tctaaaaatt ctgtaaattt atctagcatc ttttctctcc ttaattaata tttattttta
    14161 ttatcattaa aatttttata aaatcaactt tcttctaatc taacaacact gcaactacta
    14221 tttactcaac agttcttgaa cttgttttaa taactttggt cctcctaatg gactataagc
    14281 ttgtgcttgt attaatgcac atggtacact aacctcatca gcaaaagctt ttaaatagtc
    14341 aaatctatgt ctgacttgtg gagctaccat agcaatatcc catccatttt taacttcatt
    14401 ctcaaactct tgagtaccaa cagccaaaac atctatatct attccatttt ttgctccttc
    14461 tttttgtaat gcattaactg ctatagcact tgacatccct gatgaacaaa caaataatac
    14521 tctcatattg attccccctg tttttttatt tcttttcaat cattatagca tgtatttttg
    14581 attacaggaa tacgtttgta tatatttttt catattttac attttaaatt gtaattttat
    14641 ttcatgcatt tatatcttgc tttaattgct ttatttatct aatcattctt ttgtctaaaa
    14701 ttacacacta cttaaaatac tcatatcttt tcttaaaaaa gaaaaaagac tatcttatta
    14761 ttaacataaa atagtctcaa tactttttta aatatgtatt cctttatata tccagaagta
    14821 acctattatt gcaaatgata aagcaaataa aatatacagt ctaaaaaatc ccaaagaaaa
    14881 acctctctta gtttcaacaa agtatcttgt atatgcaact gcaaaagatg taattaatga
    14941 atttcctata acaactccaa atctattatc tattccaaat atagataata acaaaggaaa
    15001 aactacagta cttacaagag atgaaactat tagtattaat ccacttttaa aacttatagt
    15061 tggaatatta taatctgttc tttgattttt ctccatttgt ttcttagtat ttttattact
    15121 ttttgtactt tttgcatttt tataattatt atttttcttt cttttctttt ttttattatt
    15181 agacatgtca tttacctcca aaaggtttag gtgttatatt ccctaaaata actttatttt
    15241 tagcccctgt tgcagatatt acattagaaa aagaattatt taatgtttca ttgcccaaga
    15301 ttacaaatgc caaagcctcc tttgcatcag aagaataccc atattcatct tgagttaata
    15361 cttcaacctc taataataac tcttttatat atcctattaa agttagatta tgagctcctc
    15421 cacctccaat taataaagta tctacattaa cctttggaag tatgaagttt ttataattat
    15481 aatatatagc ttttgccgta aacatagtta ctgttgctat caaatcatat ttatctacat
    15541 gtttatattt gttaagcatg ttatccaaat aaacttgtcc aaatacctct cttccagtgc
    15601 ttttaggtgg cgcttgacta agatacttat gactcatcaa atcttttaac atttcttcat
    15661 taattttacc ttttgatgca aagtatccat ttttatcata ctttctatta aataatcttt
    15721 gacatactcc atctattatc atatttcctg gtcctgtatc aaatgcaaat acatcatcta
    15781 tgttacatga tttaggtatt atagtaatat ttcctattcc accaatattt tgaagtgcta
    15841 aatttttatt gttatcacta tataataaaa attcactata tggtactaat ggagcacctt
    15901 ctccacctgc tgctatatcc attactctaa aattagatat tacctttgta tttgtttcat
    15961 atgctataac tgctggttca cctatttgaa gcgttgaaga tacaaaatta ttataacttc
    16021 ttggtatatg aaagattgtt tgaccatgag atgcgataaa atccaaattt gctatatgaa
    16081 agtttgcttt atgacacact gattttacag ctttactaaa taaatatcca agcttaaaat
    16141 ttaagctaca gattaaatca acactcgatt cctcttctat acagcacttt cttatttcgt
    16201 cttttatatc tttaggaatt tctagagtta taaattctat ttgcttaact ttagtatttc
    16261 taccattccc actaatttca gctagtactg catcaactcc atctaaggaa gttcctgaca
    16321 ttaaacctac actatacata gttatcatcc ttttaagttt atagttattc ctccccaaat
    16381 caaggtatta agtttaataa ttttagaatt aatcttattt catctttaat tacgataatt
    16441 tactcttacg aaattaatcg agttaatctt atacacgttt tttaccttag ctaaatctta
    16501 cattaattta ttttcagtgt caatgtaata ccatgtaatt ttttttcaca aacaaaacat
    16561 attctctctc caaaaaccat attaaaatag ttactacaaa taaaaaacat accaggtacc
    16621 tatctgatat gtttttcata tataataatt attattttat aatatagatg gagcatcatt
    16681 taaggcacta attaaatctt cactattttc aagaactaaa tttattctta ttgtatatgt
    16741 cgatttgtat atgatgatag attttatagt ctaataacct aactatctat gcttaaataa
    16801 tccgatatat cataaaagta gatattaaaa attgtatatg aaccaatttt cgaacatacc
    16861 tcaatctcat gccctaaact atctatcatt ttctttacaa aatacatccc cattccagta
    16921 gacttataca attgatttct tccatttttc cccgtaaatc ccttgtcaaa aaccctagat
    16981 atatcttcct catttatacc tataccaaaa tcttttatag ataataccac tccattttct
    17041 aacttttttg aattaaattc aatcttacca acttcctttg aatactttat agcattatta
    17101 ataatttggt ctaaaacata tgacatgcat ttagaatctg tgtaaacatc attttctaat
    17161 tcattcaaac taacttctac attatttttt ataagcaaaa atgaattatt ttttatagac
    17221 atttttacca atttttctaa attaaattta tttataaata catcttcaaa catagtgttg
    17281 cttctactac tatacaaaat agagtttact aaaaaattta tctttgcaac ctgattctta
    17341 atatccaaac tatcttctat gctacttaac ctatctgtaa ttatacttaa agaagatatt
    17401 ggaattttta tttcatgaac ccatttagct atgtactcat ccaattcttt taatgattgt
    17461 ttataagtct ctatgtcact attatttttt atttttaagt tttctataat atcttttatt
    17521 agattttctt caaaagattg tccatcaact aaataactat ctatttcacc actattttct
    17581 atacaattat ataaatttgt gtaactattt ctaaaattta tataatctat aacaaaaaat
    17641 accattatta taaacagcac taaaaaatct aaatatatta actctgaata tttattttta
    17701 aaaatatcta tagcaaataa gtagatattt attatactaa ctacaactaa aggtagagca
    17761 atatacttag acctaccttt caaatatttt actagcattg tattatatat cctatcccct
    17821 tcttagtttt tataaaatca tcaaaaccca gttctttaac tttacttctc aatctattca
    17881 tatttacagt aagtgcattg tctgtcacaa actcgtcatt atcccataga gtcatcataa
    17941 gactttccct tgatacaacc ctattcttat tttttaataa taatgttaaa atttttattt
    18001 cgtttttagt caactctata cttttatcct tatacttatg ttttataatc cctttcccca
    18061 catcaaacat caaatcttca caatacaaaa tactattaga atcactataa ttatagactc
    18121 ttcttattat accatttatt ttggtaacca atatttctat agaaaatggt ttggtaatgt
    18181 aatcatctgc tccattattt atgcccatta ttaaatccat atcagaatct cttgatgata
    18241 aaaatattaa tggtacattt gaaatagctc ttattttttc gcaccagtaa aatccattat
    18301 ataatggtaa atttacatct aaaagtacta catctggttt tttatctata aattcatcta
    18361 atactacctc aaaatcatca attaattcta ctttaaatcc ccatttttca agtgctaacg
    18421 caatctcttt tgataaatca atatcgtcct ctactaataa aattctatac atttgtatat
    18481 tctccattat cctaatattt cactatgcta tgaattatta atatcataac atattttata
    18541 tgaatttatt acccaaatta tttagttaat caacaaggag actcttatta caagaatctc
    18601 ctttctcttt attaatatat caactaattt ttatacacta cactcttaaa cttattaaca
    18661 gatactgctc catatattat gaatattatc aaacatatca ctaaacttag tatatatata
    18721 ccaaataaat cggtcatcaa taaatctcct gctaatctca atgcaaaata actagatata
    18781 gttgcactta acattggcaa agtataaaaa atagctactt ctttaagtat agctctattt
    18841 atatcttttt ctgtagtacc tatttttaac aaaatcttat atttctcttt atcttcatat
    18901 gcatcactat atattttcat atatattata cttccagcta caattgcaaa gactaagaat
    18961 aaaaacacaa gcactacata tgcaaacttc atccattgac tctcaccttg agctttatat
    19021 ccattttgaa tatacccatt ttttaaactt aaattctttt caatattgtt gaacattact
    19081 tgactatttt cttcttgaaa tacctttgca ccataaaaat acacttgttg acccttatct
    19141 cttagtttaa aatatgtatc atcatttacc actataatac tttgattaag tcctgtccct
    19201 agtactggaa cttttacttc tccccttgaa atattatata actgaccatc aagttttatc
    19261 ttggcttctt ttgtagctat tgatgctatc gtttgagggc tttttaactc tattacatta
    19321 tttccactaa ctaatctttc accatactct tttaattctt tatcattacc attcacttta
    19381 agagtattac aaaaatcact atatttcatt atagtatctg ttgtatcata agcttctttt
    19441 atatctgttt tctttaaaat tgttagttct ggatttactg agtactttat cttattagta
    19501 tcttttatta tattctctat ttgtttttta ttcacttcat ctttatccat tgaaactatg
    19561 ctaatagtgt acacatttct ttgttcttgg gcattcttat gtatatgctt catagcaact
    19621 gaagcaccaa gagttgatat tgttgtagtt ataattatag ctattatagc ataagtttta
    19681 tagtttttat ttaatcggta gtttatattg tttattgcaa ttatattatt tccattaaat
    19741 aatatttttt tatttttgat tataatatca aatataacag gcataactga tttgaataat
    19801 ccataagtac ctactattat tatacctata acaggtagta cactagttat atctccagtt
    19861 cttatattgg tagaaagata gtagccatat cctattacta taattgatac tatagctaat
    19921 atatatatac ctattcctac ctttggtatt ttttcttgtt ttttagatat attgattaga
    19981 tttattacac tacttctaca tattgttata aaacctttta cagccattat tataaatata
    20041 gaaataaaga ttaaagtagc atatagtata gcttctatac taagtccatt agataccttt
    20101 atattaaatc cagataactt tattatcact acttgaaaaa atctagatgt tattatccct
    20161 ataattattc ctgttataca agaacttatt cctataagca tattttctac aaaatatatt
    20221 tttcctatag tatataaatc taatcccata aaagacaata ttccaatttc tttagaccta
    20281 ttcttaaaaa atatatttgt tgcataccat ataaagaaaa ataaaaatac tgatagtatc
    20341 attattacag ctactaacac tgatagtgta aattttttat tttcctcttg taacacattt
    20401 atagcatcac catatcttaa taattcaaaa ttagttagca caactactga aaaaaccata
    20461 gatataaaaa atgctagata agttcttatg ttactcttaa aattattata agcaattcca
    20521 agtatactca cttacatctc acctcccata gaggctagca tatccataat ttttccataa
    20581 aattcttttc tactatttcc tttgtttaat ttacacttta tatttccatc atttatcata
    20641 tatacttcat ttgaataact tgctgttaga gggtcatgtg taatcattat aatagtagcg
    20701 ttattttctc tgtttatttt ttctaatgat tctaaaagtt catatgctga tttagagtct
    20761 aaagcaccag ttggctcatc agcaaatatg actgttggat ttgtaattaa tgacctagct
    20821 gctgcgcctc tttgtttttg tcctcctgat aactgatatg gatatttatc taaatgattt
    20881 tctaatccaa attttttagc tatagaaaat accttagctt ctatctcctt acttttcttt
    20941 tttcctaatg ctagaggaag tgctatattg tccattagtt tcatattgtc taatagatta
    21001 tagtcttgaa atataaaccc tattttttct cttcttaatt ttgatagttc attatttttt
    21061 acttttgata tattaattcc atctagtatt atttctcctg aagtttgttt gtcaagtgta
    21121 gacattaaat ttagcaatgt tgtctttcct gcaccagatg gacccattat tgatataaaa
    21181 tcaccttcat tgactgtaag agatatattt tttaatacat ttgttttaaa tccttttatt
    21241 ccataatctt ttgatatatt ttttattttt aatatttctt tcataacaat agcctccagt
    21301 tgctaatttt ttaatatata tttattttaa aggttttata taaataaatc cttaagtaaa
    21361 tattacattt taactgttag cattacactt ttgtaacata tggaagacta ttataattat
    21421 agtagaaata tcttaaaata aaagcatgat ttacttcttt gtataaacaa agaaaagaag
    21481 ctcctaagaa gcctctaatc tatacaatta ttcttctcta taaaaaacca aaatacacat
    21541 tgattgtggc aaatacagat actataaaca agtgatatta ctactatata ttaaaacaat
    21601 tattcctgaa gataattcaa taagactatc aagcaataag ctaagcatcc aaaaagactt
    21661 aataccatta atgtataatt atacccaata ttaatgctta aaaaaccagc aacagttgga
    21721 ataatcagag atgaacctcc cataaatata cttaaagcta ttaattggct actacttcta
    21781 agttttttag gagaaacttc aaatattaat ctttttgaag taattaaaat taaaggagta
    21841 gaaaacactt gaaaaacaca cagtataata atgatatttg aatcatttgt taaagcaaat
    21901 aatagaaact gaactgttat cataatagca cttaatttta ataagtttaa actgctatat
    21961 ttttttaata aataagaacc agccagatat gttggtatct ctaacaatga ctgtaaagac
    22021 cactttaaag atatctgaga atttgtagca tttaaagcta tcattttatc tattacagta
    22081 cagttatttg ctaccaccat tgagtataat aagaataaaa tacatactag taagatatat
    22141 tttttatctt ttaataattc aataatatct ttattagtta catttatttt cttattaatc
    22201 ttttcaatat caggtaataa tagtatattt ataataagta atactacaat taaaccaatc
    22261 gctacagata atccaaaata tgagaatcta agaatcaagt aagatgctac catacttcca
    22321 atagcccatc caatcgaccc aaaagtctta ataaatgaca aataattatt aatattatct
    22381 ccacaaccta ttatccatgt atcatataaa ctacagcaag tatttaataa tcctcctgat
    22441 aaagcaacaa gtaataagta tattataaaa gttgcagatt cagtagaaaa cataataact
    22501 gatgcaattc cataaactga taaactaatt aaaacaattt ttttcattgt ttgatactta
    22561 tcagctaata ctccaaataa taattgaaat aatatattgg ttattgcata actcgataat
    22621 ataattcctc tttcaaacgc tgagtaacct aattttgata agaaaggtgt gaattttgtt
    22681 gaagcaaagg caaaaataat atatgagaaa aaatagaata tgataaattt aaatttaatc
    22741 gctttattaa tcataatcaa tcaaaattga taactttaat accactattt ccttcctgat
    22801 ttaagtttga aagatgatat tttaccgtat taggattctc tacaataact aaaggaatat
    22861 ataaagtatt ttcaagtaac ttattatcaa tttctactaa cttttccccc atattaactt
    22921 cttgaccttg tttaacttct aatttaaagt tttctttttt tgttgttgca ctgtccaatc
    22981 caatatgaat aagaatttga acatcatttt gcaatttaat tcctatagca tggttagttt
    23041 gagcaataaa acttattact cctttagtcg gtgatttaag aatattgtcc tttgctatca
    23101 tagcaaatcc atctcccatt accttagttg aaaatacttt atcaggaatt tctccaagat
    23161 taattatttt tccatcaatg tatgaattaa ttgtaattgc ttcttttttt cttttaaaga
    23221 acattataga cactcctcta cataattaac tttattctaa ttatcattac aaataacgat
    23281 attaagcata ctgcaattga ggaaaaaaga gccttatagc tctttcccca ttgtagttct
    23341 ttaatactgc ttatgatttg tttaattcca acaaatttat aaatataggt aaacatgata
    23401 acaatagtta ttgtgtaaat ttggaatatc tgccctaata agactaaata gaaagcaatc
    23461 atgttttctt ttatctcttt attctttttt atattgtaaa taacatatgc actgtatact
    23521 gcactaaagt tagagattaa atttaaactt gcattaacat cattgtttaa aagttgtgaa
    23581 aatgaaatat ttgtaaataa tgatgtcaat tctaaataca ctatatataa aacaggtaat
    23641 attagaaaac aaaccataac cactgaaaga aatatatttt catcttttaa tactgctgtg
    23701 atatttttta ttttatttgt tatcatgttt ttgttctcct tatttaaaag ttggccagaa
    23761 atccttatta gccacaatca tttcatctaa gatttctttt gctacttttg cacttggaac
    23821 agttttactc atagtaaacg actgccataa tttttgataa cttttttcta tccatgcttc
    23881 tacagctaat ttttcagaag caacttgttg ttccattaaa cctttttgga aagttggaat
    23941 tttacctata gacataggtt caaccatatc ttttccaaca aggcaaggaa tttcaaccat
    24001 tgcatcatct gcaaaatttt caatagctcc attattagga acaatcagta aaaatctttc
    24061 ctgagtatta aatgctaatg cacaagctaa ttcaacaata aattgagaat gctcacttgc
    24121 atcaaaccat gtatcccttg ctgtgccatt ttttataatt ctttcacatt cttcgaatgt
    24181 atccttttct cttctatcca taatttcatt tgctcttgta taatttggat tagcatgttc
    24241 aacagtttca tcagccatta aataatattt taaataagta ttaggtaatg tagttggttc
    24301 tatttctaat aagtcctttg ctgctctcat agtttctaac caactagatt ctttgtgttg
    24361 accttgaggt gtaaattcct catatccttt tttagcacaa tattcttgta atttaggcat
    24421 taaatcattt ccttctttgt ccttaatact tgtccaccac ccgaaatgat tcaatccata
    24481 atatctaaca tcaaagtctt tacgagaatt taaacctaaa actctagcaa atatttcctc
    24541 taaacatact ggcatatcac aaatattaat aattcttgaa tttggtctta atacacgaca
    24601 tgcttctgca actatagctg ctggatttga ataattaagc atccaagcat ttggagaata
    24661 tttttccata taatcaatca tttcgattat tggacctatt gttcttaatc cataggcaat
    24721 tccacctgcc ccacaagttt cttgaccaac aaccccatat tttagtggaa ttttttcatc
    24781 ttgttcacgt aaagcatata aaccaacacg aatttgtgcc attacaaaat caatatcagt
    24841 aaatgcttct tctggatttg ttgtataact aaatttaatt tctggatttt tctctctaac
    24901 aattattgca catgcttcag caacttttct ttgtctctca tcattattat catataattt
    24961 aattgattca ataggaaatt tagcaacact ttcaattaac attgttacaa ttccagcagt
    25021 ataagtacta cccccaccag caattagtat cctctgtctt tttctttcca tctttccttc
    25081 tcctttataa taaattttca aattcttctc ttacacttgg tactgaaagc ccaataataa
    25141 tctgaatagc ttttccatta tgcactaaac catgtgcacc tactgcctta aaatcttctt
    25201 ctttagaaat aattgagtca tctttcaagg ttaatctcaa cctcgtagca cagtttgtaa
    25261 catctacaac attttctgct ccaccaacta attttatgta ttcagatgct tgttgagagg
    25321 ctttactcaa tttatttcct ttttcatctt tagtttctaa gtattcattt ttagtatata
    25381 gttttgtttc ttcactatct tcttctctac caggagtttt catatcaaac ttcttaatta
    25441 agaaagtaaa tacaaagaac cagataaatg aaaatataat ccctacaaca acttgaacaa
    25501 catatacctt ccagtgatac attccaagag gaagccaatt taatacaacc cagttaataa
    25561 ttcctccacc aaaatcacca acaactccta aggcaaacga aatcgtttgc aagcacgcat
    25621 ctaaaaatgc atgtactagg aataaaagtg gtgctacaaa taaaaatgta aattctaacg
    25681 gctcagtaat tccagttaat actgcagtaa gagtagcagg aatcattaaa cctaatactt
    25741 ttttcttttt ttctggcttt gcagttacat agaaagcacc aaatacacct aatgaaccaa
    25801 acaccttcga taatccggat aatgaaaatc cgattggata catttccttt agagtcttag
    25861 ctgaagtttg aaaatcctga atatgagtag cccaatatac tgatgtacca cctggaacaa
    25921 caacactatc ataacaaatt ggtgcatata caaaatgatg taatccagtt ggtattaaaa
    25981 tcttttgtaa gaatgcataa caccaaactc ctatatttcc actatttata aaaaacgttt
    26041 gcaaatgtct aataacattt tgaatatgag gccaaataaa acaagttaat aatgcaactg
    26101 gtatcataac aaagaaacaa attgcaacta caagtgatga gcctctaaat aatccaataa
    26161 aatctggtag ttctttatca taaaatctat tatgaatata aatgacaata cctgatatta
    26221 acaaagcacc aaccatacca gtatctaatg ttttaatacc agcaatgctt acaagtccac
    26281 ttccagcact tatttcagct gcgaaatcaa ctccaaagct ttttccccaa aaacttaaca
    26341 tagaacctac aaaataatta aaagtcaaat acgttgctaa agcctccata caagctcttg
    26401 cttgttgctt ttttgcaagt gctattggta aagaaatagc aaataataat ggtaattgat
    26461 taaataccat ccaagctcca ctagatatta caccccaaaa ttggtaccaa atattttctg
    26521 gtgtagcaag tgaacccata attgactgat tttgaaatac aattgagaat ccaaccatta
    26581 tcccagcaaa agcaaataat agtgttggtg taaacattgc ccctccaaaa cgctgtattt
    26641 tcttcatcat ataaattact cccctcctca tatttttagt ttatctttgg ccttagatat
    26701 ctattatttt aacaggttaa taaatatata caatctttag ggtacatttg gagcttattc
    26761 acagtcaaat aagtttttgc acatttttat gttcaaattc tcataaaaaa aaagaatgac
    26821 tacaatttgc aatcaatccc ttctgtaatc attctatgcc ttttataaat actatattta
    26881 ataaataata actcaatcaa tataaaatat aacattgtca atttataact agggtgcatt
    26941 tgtaaaaatg agatattagt cgctgaaata taaattgatt catcacttaa atcagtcaat
    27001 gtattatttt taaactctgt aattgaaatt attttagttc ctattaattt taatcttcta
    27061 gcaatattaa gtgtattctc attctcacca cttagggata taataatcat aacatcattt
    27121 ttactgacaa tattgtaaaa agaatcatca aatgttcttc cctcaaaatc atatattatt
    27181 tcttgacatg ataagaacat tctctttatc tgttttgcaa ctacattttg aatatcacct
    27241 gaaccataag cgtatattgt tcttgattca taaagaagtt tacacaatgc atcataatct
    27301 ttattttttt gatcttcaac atacttaatt attgaattac atgcaatatc aaatgactga
    27361 ttttctatga catgaacctt ttctgattcc cagcttaata atactttaaa ctcactgaaa
    27421 ccttttaaat ctaatttttt aacaaatctt gtaatagttg cacgagacac attacatttc
    27481 atagcaagtt catttattga catcttacta caatgttttt tatttgcagt tatatatttc
    27541 catattaata aatctgtatc attcaattta ttataattta aaaaaatcat ttcttctaat
    27601 ctcataaact attaccccct ttaattaaaa atcgtcataa atattatacc aatattcaac
    27661 attaaattac agcgtattct tatatttact atttagccca taaaattgac tataaaaaat
    27721 actgctataa ttgatgttta aaaaaatgta gttttaattt tattctttta tctaataatt
    27781 aatattttca agttctagtg cttttcttat acaattatca tctttaaaat cagcagtcct
    27841 tttacaatct tttactttaa attctggaat agttttaaat tcctcatcac atattccatt
    27901 cttatttaaa tacatacaac ttgccattct aactattaga ccactatttt ttaaattaaa
    27961 caatacaggg tctatacacc ctccatctcc gccagtagtt tcaccaacta aagtcgcaaa
    28021 tttagtttct ttacaaaaca ttgcaaatga ctctgatgat gaatatattt ttctatcaac
    28081 aagtaagtat atattaccct taaattttga atcatctgta gattttatag tataggaagt
    28141 atcttcaaaa tcattaaaat cttttataat ttctttaggt gcattttcct ttacacttgt
    28201 tggtaaattt tctacaggat ttaattttat atttcttgca tttgtatatt gttttactat
    28261 ttcattatta tttctataaa ttctataacc atttattttg acatcatttt ttatgagttt
    28321 agatacgatt ccctgccaat aactatcact tccacctaaa ttacctctta tatcaataat
    28381 taaagcttta tgtttttcta aagtgtcaat ataatctcca attattttta aatcattatt
    28441 tatagagcca ttctttgatg acattgatgg taaatacata tatccaatct gacccttaat
    28501 aacatccttt aaaattaatt ctttttttac aaaaatatca tctggcattt ttattttaag
    28561 attcatagag tcatatctat ctacaacttt tttatcatta agaaaatcat accaatcatt
    28621 attagagtat gatttcttaa aaagttcata tcttttctta ttatctatta cttctgtgtg
    28681 tttattattt aaatcagata atatagatga catttcttct ataaattctt catcattttc
    28741 agtgtttttt attcgttttg tatattcatc cttatttgct aaccaatcaa tattattaag
    28801 cctcttattt acacctagat atggatatcc tgtctttaca acattataca cataattaaa
    28861 gtcatccacc ttttcctctt cagacaactg attcttttga gaataagtag aattacctaa
    28921 taagattata aatgctaaaa taattactaa aaataatact ttctttttca tgtaattctc
    28981 atccactttc agattttata ttttaataca ttgtatcatt attttattat cacctaaaca
    29041 gttaaaagaa attcttgaag atatattcca ctattattct ctttaaaaat ttttatttgt
    29101 attttcaaaa ttatttcatg aaagttataa aataacaatt atattcaaat atctatttta
    29161 ctgctatatc ctgaaggtaa attcttatca gtgatatact aaaaaagata tgctaaaaaa
    29221 tttatccatt ataatgactt aaatttagat actttactga tttttattta ttagttgaga
    29281 aagatattga taattaaaaa tattgataac tatagaaatg attatttttt atagaaattt
    29341 taaggatata aactaattat acttaagtct atatccttaa tttatcatga ttatgagaat
    29401 ttttacaaat acttatcttt gtccgtttta gaataaaaac aagaacatat cctgttattt
    29461 tattcaaaca tccaaattta attactacac aattacttat tagaaattgt atctacttca
    29521 tatatatatc tgttaagtat agcttctagc atttcttgtc ttccagactt attctttata
    29581 actggcattt gcagtgcata agattctaac tctttaaatc caaccttacc ttctactata
    29641 tccttaccta taccttcact aaaacttgca tatctttctt tagtaaagtt ttcaaatacc
    29701 tcatcttcta ataatttatg agctattaat aatccctttg caaatgtatc cattcctgca
    29761 atataagcta ggaataagtc ttctacctca aaagatgctc ttcttacttt agagtcaaag
    29821 ttaaatcctc ctggtgctat tccaccttgc tttaacactt catacatagc aagtgttgca
    29881 tcatatatat ttgttggaaa ttggtctgta tcccaaccta ataataggtc tccttgattt
    29941 gcatctatac tacctaaaac attgtttatt cttgctatat ttaattcatg ttggaaagta
    30001 tgtcctgcaa gtgttgcatg gtttgcttct atattcattt tgaagtattt atccagatta
    30061 tactttctta aaaatcctaa aacagtagca gtatcaaaat cgtattgatg tttagtaggc
    30121 tcttttggct taggctctat taagaattgg ccagtgaatc ctatttcctt agcataatct
    30181 acagccattt gtaagaatct tgcaaagtta tccatttcaa gttctgtatt agtgttaagt
    30241 aaagtttcat aaccttctct tccaccccag aatacaaaat tttctcctcc taagtactta
    30301 gttatttcca tagctttttt aacctgagct gctgcatatg catatacatc tgcattacat
    30361 gttgtagatg caccatgaac aaatcttgga tttccaaaca tattggcagt tccccataat
    30421 aactttatac ctgtcttttc catattaact tttatatact caactatttc atctaatatc
    30481 ttatttgtct cttctaaatc tcttccttct ggtgctatat ctctgtcatg aaaacagaaa
    30541 tactcaattc ccatcttaga cataaattca aatcctgcat caactttaat ctttgctatc
    30601 tcaattgggt ctgttatatt attccaaggt ctctctactg tgcctactcc aaatgggtca
    30661 ttaccctctc cacataaagt atgccaatag ctcattgcaa atcttaagtg ttctttcata
    30721 gtcttattac ctaccacttg ttcaggattg tagtatctaa atgctaattc attgtcagat
    30781 tttactcctt caaattttat ctgtcctatt cctttaaata tttcactcat ttcatgtcct
    30841 cctaatattt tacacatata atatatgtag tatgttttct taatttaaaa ctattttcta
    30901 tctttatttt tctttttaag ctaatttaat tatttatttt tgcattaact acttcatcta
    30961 ataacttaaa gtcatctttc aaagatttgt atattctttt atacaatcta tgatatatat
    31021 tgtatgtatc tacattttca cttattggtt ctaatttagt tgaagttttt attatagcat
    31081 cacatgcttt ttgtatattt tcatatattc cagctcctac taatgcaaga attgcaactc
    31141 caagtgctgg tccttcactt gaatttatag tatatacatc ttgattaaac atatctgctt
    31201 gcatctgtct ccatacttta ctttttccac ctccacctga ggctcttact tcattaacgt
    31261 ctatattaag attctctatt atatccatac agtctttaag actatatgaa acaccttcca
    31321 ttattgacct tgcaatttct tttttagaat ggattggaga taatccaaag aacacacctc
    31381 ttgcataagg gtcaagatga ggagttcttt ctcccatcat atatggtaag taaagcaatc
    31441 cattacatcc tgttggtact tgacttactt gattatttat aatctcatat acatcttcac
    31501 ctaaagaatt tgaaacttcc acttcacttt gacagaaatt atctttaaac cattttaatg
    31561 acaagccagc tccttgagta actcccataa catgccatgt atttggtatt gcatgacaga
    31621 aagtatgaac tctaccttct ttatcaattt ttggctcttt tgtataagca aatactacac
    31681 ctgatgtacc tatagtagat gaaactactc catctttaac tattccattt cctatagctc
    31741 ctgctgcttg gtctccagct cctcctacca ctattgttcc ttctttaagc cctgtcaaac
    31801 ttgctacatc tctagtcaca taacctgtaa tctcttgtga ttcataaact ttacctaaaa
    31861 gatttttatc tatatttaac ttttctagta cttcatcgct ccaatctctt tttcttatat
    31921 cgagcaactg cataccactt gcatctgata cctctgtagc atattcacct gtcaatttgt
    31981 atcttatata atcttttgga agaagtattt tattagtatt tttatatata tctggttcat
    32041 tgtttctaac ccataatatc tttgatgctg taaatcctgt aagtgctgga ttagcagtta
    32101 tttcaatcag tctttcttca cctactaaat cagttatctc ctcacactct tttactgttc
    32161 gttggtcaca ccatattata gagtttctca atgttttacc ttcattgtcc agcataacta
    32221 gaccatgcat ctgtcctgat aatccaattc cttttataca ttcactgctt atatttgaac
    32281 tttgaactac ttctcttata gattcgacta cagctttcca ccaatcattt gcatcctgct
    32341 ctgcccaacc tgatttctct tgtataagag gatattcata tgtacaactt tttatagtat
    32401 ttcctagttt atcaaataaa actgttttag tacctgatgt accaatatcc acacctaata
    32461 catactccaa cttcatccct ccttgaatgc cacttcataa ctatcgtttt cctatcctaa
    32521 tttttttata ttttaggtat attttacttg ctttattttt gttagtatat taaactaact
    32581 tatgaactaa taataaaata tttaattatc tttgtcaatg caaaataacc ttttattttt
    32641 gtgtaaaaag tattacaata ttattttaag gaggaatatc aaattggtta ctgataaata
    32701 cactattcga gaaatgaatg aaagattggt acttgaacaa attataaaaa atggtcctat
    32761 ttctagggca agtatagctt ctactatagg attgaataaa gcaactatat ccgctattac
    32821 caaaaaactt attgatgaat ctctagttca tgaaatcgga attggaaata gtactcatag
    32881 tggcggaaga aaacccatat tattagtatt taataaatgt gcagggattt ctttaagtat
    32941 ggacattgga tacgactata tattttcttc tctatcttac ttagatggaa ctattataaa
    33001 ttctaagaaa ttaacagata tacaagtaag taaagacaat gttatacaat tgatagatga
    33061 aattataaat agttataaca tatccaaaat agacactcct tataaggtaa ttggtcttac
    33121 tttggctata catgggatta cttgtgaaaa taaagtattg tttactccat attacaatct
    33181 taatgagatt gacttatata gcatccttag caaaaagtat gactttccta ttcatataga
    33241 aaatgaagca aacttaacag ctttagctga gaatactttt tcaactgtac ataactcact
    33301 acttagtctc agtattcaca gtggatttgg ttcaggcatt ataataaata ataaattata
    33361 tagtggcaga aatggtatga gtggagaaat tggtcacact atcatcatgc ctaacggtaa
    33421 actatgtcct tgtggaaatc gtggctgttt agaacagtat tgttcagaaa aaaaggtatt
    33481 tgaacagctt tcttctttag aaaatattcc taaaatagat tctgatatag taaagcaact
    33541 atattatgaa gataaccata atgccaaaaa agttattcat gagttttgta gttacttaac
    33601 aatagctata aataatgcta ttacaactta tgctcctgaa ataatatatt taaatagcca
    33661 aataattagt gatatacctg agatattaca gattacaaaa gatatgttag ttagctcttt
    33721 taacaaagga ataaatatag aaatttcatc attagggtct gaggcatctc tatatggtgg
    33781 ctctgctgta aatatcaaga gctttttgaa tatacaaaat ttaactttaa ttaatgaaat
    33841 ataagggtta tgactatata cagccataac cctttaaatt atacattcta aaattagatt
    33901 tattatatag tttatgcaag attagctata ttttttaaaa attgtatatc aattaagtat
    33961 taacataagt acttcgtttt aaatattaat ataatcttcc tactcatcat cagataataa
    34021 acttgcattt tgttcaacca tagaaatatt atctttacct attttctcta attttgctgc
    34081 caattccttt gtatctaaaa aagctgattc aataaccata cctaaattta gccctgaaat
    34141 aattttaatt cgactgtcat tattggctac taataaagca gcattgcatg gagttccacc
    34201 tagtaaatct gccattataa ttatttctga actttccttg tacacttcta aagctttttt
    34261 taatttttct attgttcctt ctattccatc atcttgtttc atatttacag taggataatc
    34321 tatagaatcc cctattatca tttttgcaga atttaagact tcttcagcca tattgccatg
    34381 actcatcaaa ataatactat tcaatttaat acacctcttt aaatctataa gatgtctaca
    34441 agataagttt tcttatcatc aggcgtcatt atagttgtaa tttcaattcc ttcatctaaa
    34501 agtcttttaa atgcttcttt ctcttcttca gtaacactga tattaggtct tagaactgtt
    34561 gtatcttttc tacttgacat atttcctaca tttatattat gtattttaaa tcctgcctct
    34621 acaaatggaa taaggtccac aggagactta acaattaata atacattttg tccttcatac
    34681 tttcctgcag taatattttt tatagcagtc tcttctgtaa ttatagatga cgctatacct
    34741 gcaggtgtag ccattcttaa aacttgtttt tgaacagaat tgttagctac tgcatcattt
    34801 actaccatta atcttgtaac acccaattta ttactccaca tagttgcaac ttgaccatgg
    34861 attaaacggt catctatacg aatattttta atacctttca tttccatatc taatatcatc
    34921 ctttccttat tttaaaatac ctaatgcata taaaattatt gaaacaacca ttactcctaa
    34981 aattaagtat ataggtttta tattcttttt aagagcccaa taaacaatca caactagaat
    35041 tgcaggaact aacccaggca taatttggtc taatatttct tgacctttca ttgtaaaatc
    35101 accttgttga aagttcaaag caacattagc ttttacaact gaaggtatta atgcacctac
    35161 aaccattaaa cctaaaatag atgtactctc agttaaaatc tttaaacttt tttcaattga
    35221 agatattagc ttagtacctg atgtataacc agcattaaat agtggtatac ttaataattt
    35281 caatgctaca ctcactacaa tccacagtaa gattccaaat ggacttccat ctatcgccat
    35341 attagctgct atagctccaa aaattgtcca cggaataacg acaaataaac tatctcctaa
    35401 acctgctact ggtcccatca agcctgtttt aattgaagca acagcctctt cagtctcttc
    35461 acttgataac tcctccattg ctacattaat acccaaaata taaggggcaa cccaaggatt
    35521 agtgttaaaa aattgtaaat gatttaatac agcactttgt aacttttctg gattatcttt
    35581 gtatactact tttaaaaaag gtagtactgc ataacagtat gccaaggcct gcatcttctc
    35641 ataatttgaa gaaacagaac tagtaaaaaa ccagcgccat aagacacttt ttttcacctt
    35701 ttcatttgat ttatttaatg ataccttatc actcatcttc gtccatacct cctactacag
    35761 tatattgttg ttcttgattt tccaaattct ttttgtaaat tataagtgca attgctccac
    35821 caattaaagc tacacctaat aatggtactt tcatatatac tgctaaaaca aaaccaatta
    35881 ctaagtaact aaaatactcc ttagcaggaa gatatctaag taataatcct atccctactg
    35941 ctggaagtag tcctccagct actgttaaac cacctgttaa ccattcaggt gtatatttca
    36001 aaatggtatt aattaaagaa ggtccaaata ttacagatag agcaactgga atacctgttg
    36061 tagctgctgt taatagtact cctaacattt gcatataggt tatattttta taatttcctt
    36121 ttttagctcc atcttctgct ttatgaacta gccatatacc aatagtattt ctaagaacat
    36181 ctaattgtac catcaataaa gcaactggaa tacctataga gattcctaca gatgcttctt
    36241 gcttagtcgt aatagttata aatgtggcta ctatacttgc tgtttgataa tcaggtattg
    36301 atgctccacc aaagttacta atacctaatg ataatagctg taatgtacct ccaatatata
    36361 accctgtatt tatatctcct aagataagac cagtaataaa acctgctgtt actggttgat
    36421 atgttccaaa cattgttgaa tttttttcat agtttataaa aaatccatat agtacgatta
    36481 ataataattg ccataattgt ggctccatta tgatttcacc tctcccaaaa tattttttag
    36541 ttctttaaaa ttataactgg aatcttgctt taggaaaata ggaatttctt ccagtgggca
    36601 ttcaacttca taagttttcc caccttcata actaactcca ctaaatatat tttcccatgt
    36661 atgtcctttt ggtaaatata cttttctact tctatcttta taattcatta ttggagctac
    36721 caaaatttca tctccaagca tatatgtatt atccacctcc caagttttat catcttcagg
    36781 aaattcataa aataaagttc tcataactgg tgttccatct tcatgcgctt ctttcatcag
    36841 tttttttaaa taagttttta agctctctct aatttttatg aattttgtaa gaattacttc
    36901 aacttcttca ccataagacc atatttcatt aggagctcca gttaccattg agcctccacc
    36961 tttatttgat aatggtttac tatgaggcaa tctatctcca tgcattctca aaattggtga
    37021 aaaagtagca tattggaacc atcttaccat aagttctcta aattctgggt ctttaggatt
    37081 tcctccatgg aaacctccaa tatcagtagt ccaccatggt atacctgcca gtcccatatt
    37141 taaccctgta tttacttgat tattaaaagc ttcaaaacta gaatcaatgt caccagacca
    37201 tactaaagct ccatactttt gagctcctgc ccaagctcct cttacaagag ttacaacaga
    37261 ctctatgtca ttatgtaaac catcataaat catttttaaa taatcaatag gataaatatt
    37321 accacaactt aaaacatttc cttttttata tctatagtta tcaaaatcat acacagaata
    37381 gccaggttca gctacatcta accaataata gtcaatccca agttgtttat aatttttatc
    37441 tattctatcc caaacatatt ctcttgcatt ttcatttgtc atatctacaa agactgtatt
    37501 tccttgaatt tgcattgtca ttctaacacc acgattaaca ttgactaaat agccattttc
    37561 taaataatca ttgtagtttt ctgcatcact ttgaactgta ggccatacag atactattgg
    37621 ttctactgaa tactcttctt tcaatttttt aactaattct ttaggatttt tccaataatc
    37681 taagtcaaat ttatattcac cttgcttagg ccaatgaaaa tagtcaattg caattgatga
    37741 aagcttaatt cctcttttag aatattcctt cacaacatct aaaacttctt ctgaagttct
    37801 atatcttagt ttactttgcc atagtcctaa aaggttttca ggcatcatag gaacagttcc
    37861 agtaacttga gaataatttt tatttaactc ttttgggctt tctccacaag taatccaata
    37921 atctataaaa tttgtagaaa acatcttcca ttctgtcata tttttagcaa aagatacttt
    37981 tccaattccc ggattattcc ataaaagtcc ataacctaga ctagaaatat aaaaaggaac
    38041 tgatatttga gaatttcttt gggctaactc caaaacagta ttctttaaat ctaaaaattt
    38101 atgttgatat tgtcccattc caaaaatctt ttcagaaggc tcagattcaa atctagtagt
    38161 tacttcaaat tctccactat agcttggtcg atattctctt gattttaact ttagagtaga
    38221 gttaaagtct ttggtaatct caattgtccc tacatcttct ccaccatcat catgttttac
    38281 agctcttaat cgaatatatt ctttcaataa tatttcattt ttatcattat aaaaagtaat
    38341 tctatctcta tggtctaaga cagcttttat ctttccattt ttaatgattg cacttccatc
    38401 ttcattttta ttaattgtaa tatcatcttt atttaactta ggtttttcgt taagagcata
    38461 gttttcatca acaaaatttt ggtctacaaa cgacctaata cgtaaagagt tttttcccca
    38521 tgcttctatt cgcaatagtt cattgtcaaa tcttttttca atatagtctt cagatatttt
    38581 tatcacggct acctcctaaa tcaatggtat aatattctat ctttgtgtag cttaagttaa
    38641 ataatgtcgt ttagtatcgt tatttataaa ttaggtttta tatataattc atttgtacat
    38701 taaactaact taccaataat atactttatt tgactttgtt tgtcaatcgc tttccggatt
    38761 ttagtaaaat tttctaatat attgctggaa agtcgttctt aatatctttt atttatactt
    38821 ttaatatctg tgatgatacc tatatacatc tagttttttt aaaacacata caggaataac
    38881 atttgcctat tatgtagata atatcggact tgaaaatatg tatagtgacc ttataaatac
    38941 ag
//
LOCUS       DACULQ010000015        38109 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.015, whole genome shotgun sequence.
ACCESSION   DACULQ010000015 DACULQ010000000
VERSION     DACULQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.015"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            15..1094
                     /locus_tag="JA154_03345"
     CDS             15..1094
                     /locus_tag="JA154_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAT7994892.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            1207..1806
                     /locus_tag="JA154_03350"
     CDS             1207..1806
                     /locus_tag="JA154_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994893.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(1993..2865)
                     /locus_tag="JA154_03355"
     CDS             complement(1993..2865)
                     /locus_tag="JA154_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT7994894.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            3327..4685
                     /locus_tag="JA154_03360"
     CDS             3327..4685
                     /locus_tag="JA154_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994895.1"
                     /translation="MNPFVLMFAAVITGILFGKIKFGKFNFGVSGALFTGLFIGWLAY
                     SLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAAVGLLAAKDMKAVLKK
                     YGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTGALTSSPGLAAALETA
                     GKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQEQIDKYIAYAEAGVGI
                     GHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEARDSVSGKTIPEVPFNI
                     ITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGYIGKIGVVNFRMEEKV
                     LNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVGVTAMMIGFLVGKYVF
                     KLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPFALLTKVILVIVLHKL
                     PM"
     gene            4712..6256
                     /locus_tag="JA154_03365"
     CDS             4712..6256
                     /locus_tag="JA154_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HAT7994896.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKSGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            6287..7678
                     /locus_tag="JA154_03370"
     CDS             6287..7678
                     /locus_tag="JA154_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HAT7994897.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            7871..9367
                     /locus_tag="JA154_03375"
     CDS             7871..9367
                     /locus_tag="JA154_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA dehydratase"
                     /protein_id="HAT7994898.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            9502..9777
                     /locus_tag="JA154_03380"
     CDS             9502..9777
                     /locus_tag="JA154_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994899.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            9801..11108
                     /locus_tag="JA154_03385"
     CDS             9801..11108
                     /locus_tag="JA154_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate CoA-transferase"
                     /protein_id="HAT7994900.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            11128..12246
                     /locus_tag="JA154_03390"
     CDS             11128..12246
                     /locus_tag="JA154_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HAT7994901.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            12533..13453
                     /locus_tag="JA154_03395"
     CDS             12533..13453
                     /locus_tag="JA154_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994902.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            13568..14725
                     /locus_tag="JA154_03400"
     CDS             13568..14725
                     /locus_tag="JA154_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAT7994903.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(14806..15606)
                     /locus_tag="JA154_03405"
     CDS             complement(14806..15606)
                     /locus_tag="JA154_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAT7994904.1"
                     /translation="MCKLISVSRSGYYKWLSYSKKSSDRGIKDRIIKDYIIEIHKKYR
                     GTYGRKRICTYLNKILDTPINHKRVYRLMKELGIKSIIRKKVYRRKFKSYEIYDNILN
                     REFRANQPLEKICMDITYIPIGKKFLYMNVAKDLFNGEIVAYEISTKMDTKLVNNTVN
                     QLINMNLAKDCILHTDQGSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNAPIESFFSHF
                     KSELIYLIDTTDPKEMISLINDYIYFYNNERIQLKNGMSPIEYRTHSE"
     gene            complement(15660..15977)
                     /locus_tag="JA154_03410"
     CDS             complement(15660..15977)
                     /locus_tag="JA154_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT7994905.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAFDDETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKLSTLLD
                     KSK"
     gene            complement(16114..17613)
                     /locus_tag="JA154_03415"
     CDS             complement(16114..17613)
                     /locus_tag="JA154_03415"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088850.1"
                     /note="catalyzes the synthesis of xanthosine monophosphate
                     by the NAD+ dependent oxidation of inosine monophosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAT7994906.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIEDEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            18209..19633
                     /locus_tag="JA154_03420"
     CDS             18209..19633
                     /locus_tag="JA154_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088849.1"
                     /note="CmtA with CmtB possibly forms the mannitol-like
                     permease component of the cryptic mannitol
                     phosphotransferase system, which phosphorylates and
                     transports various carbohydrates and polyhydric alcohols
                     in Escherichia coli; cytoplasmic protein; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIBC"
                     /protein_id="HAT7994907.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANESEEDSTSLEEAKAKMSDMKSASKNSEKNIEKKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLDIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            19668..21797
                     /locus_tag="JA154_03425"
     CDS             19668..21797
                     /locus_tag="JA154_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HAT7994908.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFSLASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIIK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKGKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNTKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIMSIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDE"
     gene            21827..22249
                     /locus_tag="JA154_03430"
     CDS             21827..22249
                     /locus_tag="JA154_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAT7994909.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAK"
     gene            22268..23419
                     /locus_tag="JA154_03435"
     CDS             22268..23419
                     /locus_tag="JA154_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAT7994910.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEIYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEAHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSNGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      23525..23626
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23809..24381
                     /locus_tag="JA154_03440"
     CDS             23809..24381
                     /locus_tag="JA154_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAT7994911.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKEEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            24445..25095
                     /locus_tag="JA154_03445"
     CDS             24445..25095
                     /locus_tag="JA154_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088844.1"
                     /note="similar to novel fructose-6-phosphate aldolase from
                     Escherichia coli; enzyme from Methanocaldococcus janaschii
                     shows transaldolase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAT7994912.1"
                     /translation="MIFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHEGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            25232..28171
                     /locus_tag="JA154_03450"
     CDS             25232..28171
                     /locus_tag="JA154_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter"
                     /protein_id="HAT7994913.1"
                     /translation="MRRRKMDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKK
                     NNILDSRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENL
                     NQSKIGIKDKEPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGL
                     HTIIYGETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVV
                     KGAYTGADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNR
                     KANVLIISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRV
                     NKNFIITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDY
                     IKSGYYDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLE
                     SKCFSEEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFI
                     KEQFKHVNQGLYLALAIHIEHAIARIKDGKTIINPSLDKIKASMPMEYELSRYIMGIV
                     EDLTQVTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGI
                     ECTLGIEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRC
                     KTVNRVDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNV
                     IITLCLSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAI
                     CGTIDIDYKNVPFISYNEVLGKNGINKVLEHLNSKQPVLNIENQLNNLIHEDLILYNF
                     EGISKEYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKP
                     AIAIARLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKN
                     EIKELLS"
     gene            28748..29206
                     /locus_tag="JA154_03455"
     CDS             28748..29206
                     /locus_tag="JA154_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT7994914.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            29267..29542
                     /locus_tag="JA154_03460"
     CDS             29267..29542
                     /locus_tag="JA154_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAT7994915.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            29601..30875
                     /locus_tag="JA154_03465"
     CDS             29601..30875
                     /locus_tag="JA154_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088840.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAT7994916.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            31005..32057
                     /locus_tag="JA154_03470"
     CDS             31005..32057
                     /locus_tag="JA154_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAT7994917.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            32057..33088
                     /locus_tag="JA154_03475"
     CDS             32057..33088
                     /locus_tag="JA154_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol dehydrogenase"
                     /protein_id="HAT7994918.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            33180..33992
                     /locus_tag="JA154_03480"
     CDS             33180..33992
                     /locus_tag="JA154_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAT7994919.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            33995..34915
                     /locus_tag="JA154_03485"
     CDS             33995..34915
                     /locus_tag="JA154_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HAT7994920.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            35090..35536
                     /locus_tag="JA154_03490"
     CDS             35090..35536
                     /locus_tag="JA154_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HAT7994921.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            35566..36231
                     /locus_tag="JA154_03495"
     CDS             35566..36231
                     /locus_tag="JA154_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAT7994922.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            36341..37843
                     /locus_tag="JA154_03500"
     CDS             36341..37843
                     /locus_tag="JA154_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAT7994923.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRTYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
BASE COUNT        15044 a         4253 c         7005 g        11807 t
ORIGIN      
        1 ttggaggtaa aatcatgagc agagtaaaaa atgtagtaga attgttggaa actaaaggtg
       61 ttgatgcact atatctaaca aagaaaacta atgtaaacta tataagtggt tttcctgatg
      121 aagaagcata tgcagtgatt tgcaaagatg gaaacttttt agttactgat agtagatata
      181 tggagcttgc agaaaaggtt tgcaaagatt ttgaaattat aaattggcat aattttgata
      241 gaagtgtggc aaaggcagtt aaaagtgtat gtgataaagt gggtataaag aaacttggat
      301 ttgaaagaac taatatagtg tttgataaat atgaagaact taaaaatctg attgagaagg
      361 ataatggaga attaatacct acagaaaata ttgtagagac tttgagatat gtaaaagaca
      421 aagatgaaat caaaaataca agaaaagctt gtgaaatagc ggataaggca ttagaagaat
      481 tgattccaca tataaaagct ggagttagcg aaattgaact ggctacaagg ttagagtact
      541 ttatgaaaat gaacggagca caaaatatag ggtttgaaac tatactgata tcaggagcaa
      601 agacttcatt gcttcatggt aagcctagtg ataagattat agaaaaaggt gattttgtac
      661 ttatagacta tggtgctatg tataatggct atatatctga tacaactaga acttttatag
      721 ttggaggagc ttctgaaaag caattagaaa tatataattt agttaaggaa gctcaaaatg
      781 taggggttga gaacatgaaa gctggtgtac atgcgacaat tccagatgct gagataagaa
      841 aagttgtcaa aaaatatgaa gactattact atcaaggtat aggacatgga gtcggtagag
      901 atgttcatga agaacctttc attggtaatt atggtgataa aatcatagaa gagggatgta
      961 taataacaat ggaaccaggt atttatttcc ctggatgggg tggagttaga atagaagata
     1021 ctgttttgat tactaaaaat ggtccagaaa gacttactaa attccctaaa gatttgatga
     1081 ttttagacaa ataatttaat ttatatatat ttttttacat aaaatataac ataatctcac
     1141 tattaagctc tatttacata ttatagtaaa gagggcttaa ttttattgtg agaggtgata
     1201 gtatatatgt ataaaaaaca taaatttata aaaaatataa aaaaagcctg gtataaaatc
     1261 aaattaattg atagatgcct tataattgta atggcaatat taatgtttca atctatatat
     1321 aacttatttg ttaatgaagt aaattcacaa gatactacaa ctattgatgt agtagtaaga
     1381 accacttcag cagctatatt tgggtatttt ttaagtgcta attttataaa gagaccttta
     1441 aggaagtata caaatactga tgtatcttca aatcttttta tagataataa tttatcccca
     1501 aaaggtcaag agtctaatca aaataatata atgaatgtca aaaataccat tggatttact
     1561 tcagaagctg ataattatga aaagaaaata cctattaata ataatgaagg ttttgaagaa
     1621 ggagaaacta gtgaattgca aattattata gctactgtaa tttgcatagt agcattaaca
     1681 gtactttttg ttgtaagaaa ttttacaact acaacatcag cttctttagg tactatttca
     1741 cagatgagag attttgtctc aggatgcgtg ggttttttgc taggttgtcc aagtaaacct
     1801 gattaaaaaa gtattatcaa taaaaggtat tttaaaaagt atcagtataa taatatttag
     1861 atttaaatgt atacaattat aattctgtaa cattctatat tgtattttta tactgatact
     1921 ctaaactata ctatttatta aaatatttta acattgtatt taaacaaaat attatatctg
     1981 tataatataa atttagttta tatgaataga tgccatatac tcaataaaaa gtttcatact
     2041 atgagatatg tattgatttc ttctccaaag aaccccaata tttgcagtaa ttgcacgttc
     2101 taatggtatt gaacagagat tttctgtatt ttgaaatatt tctttataca atattgttga
     2161 agccaagtca ttttctagta aataacgtat ggtagaaagt tgatttgagt gtaaaattat
     2221 atttggctta gctttttcaa gtctttttgt aataatctta tagtgataag aaccttcaga
     2281 taagattaca aaaggtacat tttttaacat ataattcgat ataataggat gttttgccaa
     2341 ttcatttttg ggatttgtaa ctaagtataa ttctgtttta tagattgtct ttgaatctat
     2401 atcatcataa atatcactgt caattatacc aatacaaaaa tcaagtgtag attcttcaat
     2461 cattttagct ccaacaaatg ttggaacttc aaaaatttta agctctatat ctggataaat
     2521 tacattaaac ctaggaagta tttttgaaag caaaaacgta cctaaaactg taggaatacc
     2581 tattttgaga gaagcttttc tcttttttcc taaatcaagt gcgtcacatt gaaaatcctc
     2641 aaattgtttt ataaaatgtt ttgacttgtc taaaaaaatt tttccttcag gcgttaactc
     2701 tattttatta cctattctgt aaaaaagttc aaagccaagt tctttttcta attctttaat
     2761 tgctaatgtt atggatggtt gagaaatatg aatactttcc gcagccttag tcatgttttt
     2821 atatttacag acagtttgaa aatagtataa atagtttata ttcatttggt attagttcct
     2881 ttcatttaca tattagtgac aagtatataa ttaacaatag cataagtttt gtatattgtt
     2941 gtaaaaaaat agaatagttt taaactatca tagcataagt ttttgatatt ttcaatctat
     3001 taagaatagt gctattatta aattaacaaa agagaatagc tggtatggtt tagagtaaat
     3061 caatctatat tacagttata tcaacgaata tcaaaaaatt atctattaaa aaataaaaat
     3121 tatttttaca attaaattgt taattaaatc ataaagaaaa aagtgatatt aacaagataa
     3181 aattttttta atcagaaatg ttaatttaat aacaaaatag agtattgggg agaaaggaga
     3241 aatatatttt ttaataatat ttgttaattt aataacgaaa aatgaaaggg agtgatttag
     3301 tgaagtttga attgatggat tttttaatga atccttttgt ccttatgttt gctgcagtaa
     3361 ttacaggaat tttatttgga aaaattaaat ttgggaagtt taattttggg gtatcaggag
     3421 cattatttac aggattattt attggatggc ttgcatatag cttggggaat ctaataatag
     3481 caaaagggga aacagcagca ggatataagg cagcaactgt aatgatgggg aatggaatta
     3541 tttcttctga cttctttgat ttctttttaa taatatttgt tgcagcagtt ggtctattgg
     3601 cagcaaaaga tatgaaagct gtattaaaga agtatggagc aagatttgta attttaggag
     3661 ttcttataac ttttataggt ggctctatga cttatgcaat gactttacta tcaagtgata
     3721 aaggaagtag tgcttatgaa gtttctggcg tttacacagg agcattaaca agttcgcctg
     3781 gattagctgc tgctttagaa acagctggta aacatgcaga agatgtatca aaagaatttg
     3841 aaaaagcttc aataaaagat aaaaaagaaa ttttaaaagt agtcgaccca gaaggcaaat
     3901 tggatgtgaa tacaactaca tcactgactc aagagcaaat agataagtat attgcatatg
     3961 ctgaagctgg tgttggtata ggtcatgcag tcgcatatcc ttttggagtt ttaatagtaa
     4021 tcttaggtgt aaatttcctt ccaaaattat ttaggatgga cctaaaagaa gaaagaagaa
     4081 aatatgagaa ggaaatgaag gaagcaagag atagtgtttc tggtaaaacg ataccagaag
     4141 ttccatttaa tataataaca tttttcttga catgtttagc tggatactta gttggaggaa
     4201 tacatgtatt tatgggacca ctagggtact tcacactagg tgcaacagga ggttcactaa
     4261 ttgtatctct agttcttgga tatataggta agataggggt agtaaatttc agaatggaag
     4321 aaaaggtttt aaatatttta aaacaaatag ggctagtgtt tttcttagca atagttggat
     4381 taagatatgg tggaaaagta gttgattcaa ttatgacatc tggtatgcac ctagccttag
     4441 tagcaatagc agtaggtgtt acagctatga tgataggatt cttagttggc aaatacgtat
     4501 ttaaattaaa ttggatttta ttatctggag ctgtctgtgg tggtatgaca tcaacaccag
     4561 gtcttggagc tgctgttgat gctcttgata gtgatgaccc agcagcaggt tatggtgcta
     4621 cgtatccatt tgcattatta actaaagtta tattggttat agttttacac aaacttccaa
     4681 tgtaaataag attttatatg gggggatatt tatgaataat gaaggagcat ttgaagaatt
     4741 aagtggaaga ttaagaaata aagaattgtg tgatttaata atgaatgcag atgaagctgc
     4801 taaaattata gaggatggca tggttattgg agttagtgga tttacacctt caggctatcc
     4861 aaaagcagta ccattagcag tttcagaaag agctaaaagt ggagaaaaga taaaattaac
     4921 cgtatattca ggagcatctt taggtccaga agttgatgga gcatggtcag aagcaggtat
     4981 aattgaaaga agacttcctt atcaaactaa ttcaatactt agaaataata ttaataaagg
     5041 tgttgttgat tatatagata tgcacctaag tcattctact caatttttaa attatggaac
     5101 tatacctaaa gtagatgtag ctatagtaga agcacttgca ataactgaag aagggaatat
     5161 tataccaact agtgggatag gtaattctcc atcttttata aaaagtgcag ataaagtaat
     5221 agtagagata aatcttgcaa aaccaatgga aatggaaggt atggcagata tatatattac
     5281 ggaaaatcca ccaaatagaa aaccaataga aataaatcat cctaaggata gaattggaac
     5341 aacatatata ccttgtggtt tggataaaat tgcaggagta gtaattacca atatgcaaga
     5401 taagacccgt ccattggggg ttgtagatga agcttcgaaa aaaatatcaa ataatattat
     5461 tgctttttta agagaagaag taaagtcagg cagattatca aagaatttac ttcctcttca
     5521 atctggtgta ggaagtgtag caaatgcagt attatatgga ttatgtgaat ctgaatttga
     5581 aaatttaact tgttatacag aggttgtaca ggactctatg ttggatttaa taagaatggg
     5641 taaagttaca atggcatcta ctacatctgt aagtccatct ccagaaggat taataaaatt
     5701 tgaaaaagat attgattttt ttaaggataa gattatatta agacctcaag aaattagtaa
     5761 caatccagaa atagccagaa gaattggtgt aattgcaatg aacactgcaa ttgaagttga
     5821 tatttatgga aatgtaaatt ctactcacat aatggggtca aagatgatga atggaattgg
     5881 tggttctggt gattttgcta gaaatggagc aataactata tttagtactg agtcaattgc
     5941 aaaaaatggt gatatatcat caatagtacc tatggtatct cacgtagacc acactgaaca
     6001 tgatgtaatg gtaattgtaa cagaacaagg atatgctgat ttaagaggtc ttgcacctag
     6061 agaaagagct ataaaaataa tagaaaattg tgcccatcca gattataaag aacaacttag
     6121 ggattattta aatagagctt gtaaaagtgg agcaaaacaa acacctcaca tattggacga
     6181 agctttatca tggcatagta aatttatgag tacaggtaca atgaaaaagg ctgaaacttt
     6241 taaaagtgca ttataaaata tttaataaat tggaggtaga ttttaaatgg aaaaagcagt
     6301 ggaaaacttt gaagatttaa gtaaggaata cattaatggt tatattgaga gagcaagaaa
     6361 agctcaaaga gagtttgaat gttatactca agaacaagta gataaaatag taaaaatagt
     6421 tggaaaagta gtgtattata atgctgaata tttagctaaa cttgctgtag aagagactgg
     6481 aatgggagta tatgaagata aggtagctaa aaataaaagc aaagcaaaag ttatatataa
     6541 caatttaaaa gataaaaaat cagttggtat aattgatata gatagagaaa ctggaataac
     6601 aaaggtagct aaaccagttg gagtagtagc ggcaataact ccttgtacaa atccaatcgt
     6661 tacacctatg agcaatgcaa tgtttgcact taagggaaga aatgcaatta ttatcacacc
     6721 tcatcataaa gcaattggat gtagtacaaa aactgtagag atgattaatg aagaattaga
     6781 aaaaatagga gctccagaaa atttaataca aatacttgac caacaatcaa gagaaaatac
     6841 aagaaattta atttcatcag cagatgtagt aattgccact ggaggaatgg gaatggtaaa
     6901 agctgcgtac tcaagtggaa aaccagcact tggtgtaggg gcaggaaatg ttcaatgtat
     6961 catagataga gatgtagaca ttaaagaagc agtacctaaa atcatagcag gaagaatttt
     7021 tgataatggt atcatttgtt caggtgaaca gtcagtaata gtagcagaag aaatgtttga
     7081 taaaataatg gatgaattta aaaataataa aggttttata gttagagata aagtacaaaa
     7141 agaagctttc agaaatgcta tgtttgtaaa taaatctatg aataaagatg cagtaggtca
     7201 atcagttcat acaattgcaa aaattgcagg tgttgagata ccagaagata caaaaataat
     7261 agtaatagaa gctgatggac caggagaaga ggatattatt gcaaaagaaa aaatgtgtcc
     7321 agttatatct gcatacaagt ataaaagttt tgaagaaggt gtagctattg caaaagcaaa
     7381 tttaaatgta gaaggaaaag gacatagtgt atctatacat tcaaatacag ttaaaaatat
     7441 agaatatgca ggagaaaata tagaagtatc aagatttgtt attaatcaat gttgtgctac
     7501 tagtgctgga ggaagtttct ttaatggtct tgcacctact aatactctag gttgtggttc
     7561 ttggggaaat aacagcatct ctgaaaatct tgattataag catctaatta atatttctag
     7621 aattgcttat tatatgccag aaaatgaagt ccctacagat gaagaacttt ggggataaaa
     7681 tttagacaaa aaatattcat tctaaattaa agtaggaata tattttaatt tagaatgaat
     7741 ttaagacata aattaaattt aaattagttt attggactaa atttaaaata ttaagagaca
     7801 tatagaagaa ggaagtatta taatttaaaa taaataaaaa aatctataaa agagaaggag
     7861 tggttttatt atggcattaa tgacaggagc tcaatatatt gaaagtttga gaaaattaaa
     7921 tactaaggtt tatatgtttg gtgaagaggt taagaattgg gttgaccatc ctatgattag
     7981 accatctata aattgtgtag cagcaactta tgacttagct catgacccag aatatgcaga
     8041 tttaatgaca gttacttcaa atatcacagg tgaaaaaata aatagatttg gtcatcttca
     8101 tcaaagtgtt gatgatttga taaaaaaagt aaaaatgcaa agactatgtg gtcaaaaaac
     8161 agcttcttgt ttccaaagat gtgttggtat ggatgcgttt aatgcagtgt attcaacaac
     8221 atttgagtgt gataaagccc atggaactaa ttatcatgat aattttgtaa aatacttaac
     8281 ttatatacaa gaaaacgatt tagtagttga tggagctatg actgacccta agggagatag
     8341 aagtctatca cctagtgctc aacctgaccc agatatgttc cttcatatag tagaaagaag
     8401 agaagatggg ataattgtaa gaggagcaaa ggcacatcaa actggttcaa taaattcaca
     8461 tgagcacctt ataatgccta ctataagtat gactgaagca gataaagact acgctgtatc
     8521 atttgcagtt ccttcagatg ctgaaggagt gtttatgatt tatggaagac aatcttgtga
     8581 cactagaaaa ttagaagaag gtgcagatgt tgatttagga aataaggagt ttggaggtca
     8641 agaagcttta gtagtatttg ataatgtatt tgttccaaat gatagaattt tcttatgtgg
     8701 agaatgggat ttctctggta tgttagtaga gagatttgca ggataccata gacaaagtta
     8761 tggtggatgt aaagtaggag taggtgatgt aataatagga gcagcagctt tagcagctga
     8821 ttataatgga gcaaacaaag cttcacatat aaaagataaa ctaatagaaa tgactcacct
     8881 taatgaaagc ttatattgct gtggtatagc atgttcatca gaaggtcaca aaacagaagc
     8941 tggaaactat caaatagatt tacttcttgc aaatgtgtgt aaacaaaatg taactagatt
     9001 tccttatgaa atagtaagac ttgcagaaga tatagctgga gggcttatgg ttactatgcc
     9061 ttcagaaaaa gactttaaat cagaccttaa agttggaaca agtggaatga ctattggcga
     9121 agtatgtaac aagtatttta aagcttcttc agtggcttca acagaagaaa gaatgagaat
     9181 attaagattc ttagaaaata tatgccttgg ttcatctgct gttggttata gaactgagtc
     9241 aatgcatggg gcaggttcac cacaagctca aagaataatg atttcaagac aaggaaacat
     9301 aaatcaaaag aaagaattag ctaagaaaat tgcgggaata aaaaaagagg aagctttaaa
     9361 tttataaaaa gttgatatat aaagggaaat tatatattaa tgtgtattaa ttaatgaaaa
     9421 aatatgtagt ataagagata gttataaaat tatggtggtt tttagctaag taaagaaatt
     9481 tttaataagg agaatttaca catgacctgt ggagttagat tttgtggggg ctgtaaccct
     9541 agatacgaaa gagggaaggc tcttacaata atcaaagagc acttcaatgg tagggtagat
     9601 tttagtatag cacaagagaa cgtcgagtat gatttgctac ttataattgg aggatgcgca
     9661 actctatgcg catcctatca acaatataaa gtgaaaaatg aatgtgttaa gatgtgggat
     9721 tttagccata tagaaagagt tataaatgtg attgaatcag caatttgtga aacttaaaaa
     9781 agctaaatgg ggggatttca ttgagttggc aagaattgta tcaaagtaaa ttatgtagtg
     9841 ctacagaagc tgtaaaacag attaaaaacg gtgatactgt agtatttgca cattgtgtag
     9901 gtgaacctcc tgcactagta gaggctatga ttgaaaatgc tgaacaatat aaagatgttg
     9961 agattaaaca tatggtaagc ttgggtagtg gtggatatac tgctaaggga atggaagctc
    10021 attttagagt aaatccaatg tttgtaagtg gaaatgtaag aaaggcaatt gaaaatggag
    10081 atggtgattt tacacctgca ttcttccatg aagtaccaaa gttgcttaga gaaaaaagat
    10141 taaaatgtga tgttgtttta gcacaggtaa ctccaccaga tgaacatggg tattgtagct
    10201 taggaacatc agttgattat acttatgaag ccattaaaac tgctagaaca gtaattgttc
    10261 aagtgaatga ccagtttcct agaacctatg gtgaggtagt ccatgtatca gagtttgact
    10321 atatagtgga aaaatcacaa cctttatttg aactacaacc tgcaaagatt ggagaagtag
    10381 aagaagcaat tggtaaaaat tgtgcttcat taattgaaga tggttctact ttacagctag
    10441 ggattggtgg gataccagat gcagttatgt tatttttaac tgataaaaag gatttaggga
    10501 ttcattcaga aatgatttca gatgggactt tggcccttta tgaaaaaggt gttataaatg
    10561 gtaaatataa aaattttgat aaagaaaaaa tgacagttac cttcttaatg ggtactaaaa
    10621 aattatatga ctttgcaaat aataaccctg cagtagaggt aaaaccagta gactatgtga
    10681 atcatccagc aattataatg aaacaacata agatggtttc tataaattct gctatacaag
    10741 ttgatttaat gggacaggta gttgcagagg ctatgggact tagacaattt agtggtgttg
    10801 gaggtcaagt tgactttata cgtggagtgt ctatgggtga agatggaaag gcaattatag
    10861 ctatgccttc aataactacg aaaaaagatg gtacagtaat ttcaaaaatc gtgtctattg
    10921 tggatgaagg tgcacctata acgacatcaa gaaatgatgt tgactatatt gtaacagaat
    10981 acggtatagc agaattaaaa ggaaaatctc ttagagaaag agcgagaaat ttaataaata
    11041 tagctcatcc aagtgtaaga gaaagtctag cagtagaatt tgaaaagaga tttaaggaga
    11101 aatattaata atagaggggt gttaataatg gaagcactta gagtagttcc aaagatattt
    11161 tactttgata catttaaaga atttaatgaa gaatttaaaa taggaaaaaa tgatttagta
    11221 attactaatg agtttatata tgagccatat atgaaacctc ttggaataga tacaaattta
    11281 atttttcaag aaaagtttgg aacaggtgaa ccatctgatg aaatgataga ctccatgaca
    11341 aaggaaatga agaaatacaa ttttgataga attattgcat ttggaggagg aacaatagtt
    11401 gatatatgta agatattagc acttgatgtt ccagaaaagt ctattgattt atttgaagga
    11461 gaagtatcac ctaaaaaagt aaaagagtta gtagtagttc ctacaacatg tggtacaggt
    11521 agtgaagtaa caaatgtagc tattgcagag ttaaaatcta aacatactaa aaaggggctt
    11581 gcagtagaag aaacatatgc agactatgca gtgttaattc cagagacagt aaaagggtta
    11641 ccttacaaat tctttgttac aagttcggta gatgctctta tacatgctat tgagtcttat
    11701 ctttctccaa aagcaagtcc atttacagag atgtattctc tacaagcaat aaaaatgatt
    11761 atggatgggt ataagaagat agttgataaa ggagaagaag agagatttaa tcatcttaga
    11821 gactttgtac ttgcatcaaa ttatgcagga attgcttttg gaaatgctgg ttgtgcagca
    11881 gttcatgcac tatcttattc aataggtgga gctttccatg ttgcacatgg tgaagcaaat
    11941 tatcagttct ttactgaagt atttaagatg tattctagaa aaaaacctaa tggaaaaatt
    12001 ataaaatgta caaaaatttt agcagatgcc cttgaatgtg accctaactg tgatgtttat
    12061 ggagaattag aaaagttttt aaataaatta atagctaaaa aagctttgag agaatatgga
    12121 atggtggaaa gtcagataga tgaatttaca gatagtacaa tagcaaatca acaacgacta
    12181 cttgctaata attatgtaga gcttagtaga gaggaaataa gagagatatt tgcaaatctt
    12241 tattaataca attttttatt tatatgtata gtcaacataa acaatgctaa ttatatttat
    12301 gtacaaataa aaataaggga acttaaattt taattaaagt taagttctct tatttttata
    12361 tggagttaaa tataagtata tttttaattt aaaatatgct tatatttaag tctattactt
    12421 tatttgaaac aatttgtaga atttcaaatt ccttgacagt aataagtaag taattgtata
    12481 atcaaactgc aatatattaa taatattagt gaaaatgaaa ggattataaa atatgtataa
    12541 atcagaaaat ttttcaaaaa aatctgtatt aataaattca tttttaagta aatttttaaa
    12601 aataattgga tggggtttgg taagcttatt tacattatca gccttagtac tttcaactat
    12661 ttccattata attggatggg ctgtaatact tcctattcaa gtatgtgcag tatttttact
    12721 tataggagga gtttgtattt ttaaaggtaa gagattagga gaccaaattt ctcgttataa
    12781 aaaatattgt acaattataa acaatagaaa tattattcca gtagaactta ttgcagaaaa
    12841 cacatctaaa tcattaaatt ttataataaa agatgtacaa aagatgatag acaaaaacta
    12901 ctttataaat acttatattg ataaaagaaa taatcagatt gtgttaacaa atgaagattt
    12961 tgttccacca agatatgaag atgtagcata tgaagtcaaa gaagaagaaa agactgatga
    13021 atccaatgaa gttgatacaa ttataaataa aggaatgaat tatctaacac aaataaaaga
    13081 agctaataaa aatattaaat cagaatcaat gggcgataaa attgtacaag tagaagatgt
    13141 tacaagtaaa atatttgatg ttgtaaaaca tgacccatca aaacttactc aaatacaaaa
    13201 atttatggat tattatcttc caaccacttt aaaactatta aattcatatc atacattgga
    13261 agaacaagga attgatagag aaaatataac aacaactatg gaaagtattg aaaatacaat
    13321 gaatacgatt gtagtagctt ttgaaaatca attggactat ctgtttgaag atgaagccat
    13381 tgatatttct acagatatta ctgttttaga gaatatgtta gtccaagaag gattaacgag
    13441 tggtaaattt taacttcaat agaaaaaata ttctgttatt tttaaacaat taattagtca
    13501 taaagtataa aatgtgtatt atataagtta atcagtactt tatgaaggaa gaggagagaa
    13561 agatacaatg aataatttag atgatatacc agttatgcca actttgacac ttgacccatt
    13621 tggagaagaa tcaaacacta atgatataga taattctgac ttattaatga aaaaggatga
    13681 aaaagaccca gaagaagaaa aactgtctga atctgaacgc aagatggtaa aggaatttgc
    13741 ggaaaaaata gatattacaa atacaaatat gattttgcaa tatggagctg gtgctcaaaa
    13801 aaaggtagca agtttttctg aaacagcttt aaagagtgta aagacaagag atttaggaga
    13861 agttggagat atgcttacaa atctagttac tgatttaaaa gcattttcag cagatgaaaa
    13921 agaacaaagt ggttttttag gaatttttaa aaaggcaaat aataaaattt caaatttaaa
    13981 aactaagtat gatagtgcag aggtaaatgt ggataaagta agcaaagagc ttcaaaagca
    14041 tcaggttaaa atattgaaag atatagcaat gcttgataag atgtatgaat tgaacttagc
    14101 ttactcaaaa gagcttacta tgtatattat agcaggaaaa caaaaattaa aggatatgaa
    14161 agaatatgag atgccaaagt tgagagaaaa agctcgttta tctggttcaa cagaagatgc
    14221 tcaatctgta aatgatatgg tttcactttg tgacagattt gaaaagaaga ttcatgactt
    14281 agaattaact agaatggtat cgcttcaaat ggctcctcag ataagattgg ttcaaaatag
    14341 taataatctg atggcagaaa agattcaatc aactatagta aataccattc cattatggaa
    14401 aaatcaaata gtgcttgctc ttggaatttc tcacttaaat caagctatga aagcacagcg
    14461 tgaagttagt aatatgacaa atgagctttt attaaagaat gcagaaactt tgaagatggg
    14521 aacaatagaa acagcaaaag aatcagaaag aggaattgta gatatagaca caattaagaa
    14581 aactaatcaa tctctaatat ctactattga tgaggtagtt aagatacaac atgatggtag
    14641 aataaagaga caagaagcag aagttgaatt atcaaaaata gaaaatgaac ttaaaagcaa
    14701 gcttcttgaa tttacagtta aataaaaatt atactaagat aaaaagagac tgtctcaaaa
    14761 ttggatatgt ccctgtcaag tagacagact taaaaaaggc tatctttact cggaatgagt
    14821 tcgatattca atcggactca ttccgttttt taattgtatt ctttcattat tataaaaata
    14881 aatataatca tttattagac taatcatttc ctttggatct gtagtatcaa ttaaatatat
    14941 taattcagat ttaaaatgac tgaaaaaact ttctatagga gcattatccc agcagttgcc
    15001 ccttcgggac attgattgta taataccatt atccttaagc cttttagagt aagatctact
    15061 tgtatattgt gaaccttgat ctgtatgtaa aatacaatct ttagctaaat tcatatttat
    15121 tagttgattt actgtattat taactaattt agtatccatt tttgtactta tctcatatgc
    15181 cactatttca ccattaaata aatctttggc tacattcata tataagaatt ttttaccgat
    15241 aggtatgtat gttatatcca tacaaatttt ttctaatggt tgattagctc taaattcacg
    15301 atttaatata ttatcatata tctcatatga tttaaactta cgtctatata ctttctttct
    15361 aataatagat ttaataccta gttctttcat caacctatat actctcttat gattaatagg
    15421 ggtatcaagt attttattta aataagtaca aattcttttt ctaccataag ttcctctata
    15481 ttttttatgt atttcaatta tataatcctt aataattcta tctttgatac ccctatcgga
    15541 agatttttta gaataactta gccatttata gtaaccgctt cttgacacac ttatcaactt
    15601 acacatacta gaaatactat acttcctaga cagaagttta attgttttaa atttatcgct
    15661 tactttgact tgtccaatag ggtacttaac ttttttaaga attcattctc catgcgtaag
    15721 tacttcaatt cttcttcaac agatttaaat tttctattat cattttctag actaatattt
    15781 ttaaatctgc ccctagtttc atcatcaaat gcagttttgc ctttattttt ataatctttt
    15841 acccagtttt gaacttgtgt tttgcttctt atattcagca tctcagcaat tttgtatgat
    15901 gagtatcctt cttttatata taagtttaca gcttttagct taagttcttt gctatatttt
    15961 ttgtgttttt tagacataga aaaaccccct tagagtatac gctttttaga cctattataa
    16021 tagcctttta tttaaatgtc tactataagg ggattatact aaaatagaga taagtctctt
    16081 tttcattgtt taaatgatta aagtatattt atattataaa tttctattat cttttaacat
    16141 aacatcatga gctccacctt caactatact agtagaagat acaagtgtaa gtttagcatt
    16201 tttttgaagt tcaggaatgc taagtgctcc acagttacac atagtagaac gaactttact
    16261 taaagaaaga gttacattgt ctttcaaaga accagcataa ggaacataag agtcaacacc
    16321 ttcttcaaaa gaaagtttct tatctccacc aaggtcatat ctttgccagt ttctagcacg
    16381 agcagaacct tctccccaat actctttcat ataactacca tttatattaa ctttgttagt
    16441 tggactctca tcaaaacgag agaagtatct accaagcatt ataaaatctg cacccatagc
    16501 taaagctaaa gttatatgat ggtcataaac gataccacca tcagaacaaa ttggaatata
    16561 aatacctgtt ttttcaaagt attcgtccct agcttgagca acttcaatta tagaagtagc
    16621 ttgtccacgt cctatacctt tttgttcacg agttatacat atagaaccac caccaatacc
    16681 aactttaacg aagtcagcac cagcttcagc taggtaaagg aatccctcac ggtcaactac
    16741 atttccagct cctaccttaa ctgtatcacc atatttttca cgaatgaaat caagtgttat
    16801 tttttgccac tcagaaaaac cttctgaaga atctatacat aaaacatcag caccagcttc
    16861 aactaaagca ggaacacgtt cagcaaagtc tctggtatta ataccagctc caactacata
    16921 tctcttagaa gaatctaaaa gttcaagtgg attttcttta tgagaagagt aatctttacg
    16981 gaataccata tacataagat taccattgtc atcaagtaca ggaagagaat taagtttgtg
    17041 gtcccatata atgttgtttg cttcctttaa agtaacatct ttgttagcat aaactataga
    17101 tgacatagga gtcataaatt cactaacttt agtatctaaa tccatacgac ttatacgata
    17161 atcacgactt gcaacaatac ctaataattt accagttgaa gttccatctt cagtaacagc
    17221 tactgtagaa tgtccagttt tttcttttaa gtcaagtatg tcttttaatg tgttatcagg
    17281 tttaatattt gaatcactta caacaaaacc agctttgtgg gatttaacac gtgctaccat
    17341 agcagcttca tcttcaatag tttgagaacc gtatataaat gaaataccac cttctttagc
    17401 aagtgcaata gccattttgt catcagaaac agattgcata atagcagaag tcaaaggtat
    17461 attcatataa atatctgctt cctcgccctt tttaaattta gtaacaggtg tttttaaact
    17521 tacattagca ggtatacaat cagtagaaga gaaacctggt acaagaaggt attcactgaa
    17581 agtatgtgat ggtgtatcaa aataaaacgc cattttttaa tcactcctta tatttttata
    17641 ctttttataa ttgatagtat taattataac ttattcttta attttattaa agtattttgt
    17701 caaatattga gatttttgaa aaatttaaac tattttaatt gaatttgttt aatatatatt
    17761 gccttatagg gtaaagttgt atattaaata atgtttagtg tttaatagta gctaatttga
    17821 aattaatctt tagttattca tataattaca tttgtatagt ttatattgat aatattgcta
    17881 gagagtgcat aaacctcaaa aatacagata aattaaatct atattagtat atccaaaagt
    17941 acatttaata aatttattta actgaattat tattttcatt tctattaaat ttaaatattt
    18001 tataaaaata gtattattta caaaatcata tactccaaaa tactacataa ataaaattaa
    18061 acaaaaacta aaatgacaaa tgtcattatc taaagtgaca tagaaacggt ttcttaaagt
    18121 tgttactata aaatataaac tctataataa aagtataaca aaagggaatc atatcgatat
    18181 agaatataat tcaagaggag gtaaaattat gagtagttct aatcaatcga caataaagac
    18241 atctatacag cgagtaggga aatttttaag tggtatggta atgccaaata taggagcttt
    18301 tatagcatgg ggtcttataa cagcattatt tataccaaca ggatggatgc ctaatgaaaa
    18361 attgagtact ataggcgacc caatgataaa atacctttta ccactattga tagcttatac
    18421 tggtggtaaa gcaatagcag gacaaagagg tggcgtaata ggtgcagcag cagctatggg
    18481 agtaattgtt ggggcagata taccaatgtt tatcggagca atgataatgg gaccatttgc
    18541 aggatgggta attaagaagt tcgataaatt tgtagatgga aagataccaa caggatttga
    18601 aatgttagta aataactttt caataggtat aataggtatg cttcttgcaa tactaggatt
    18661 ttatgcaatt ggaccagcaa tagtagcagg gacagctctt atagaatctg gagttcaatt
    18721 tatagttagc aaaagtttat tgcctttagt ttcagtattt atagaacctg gaaaagtatt
    18781 attcttaaac aatgcaataa atcatggtat attagggcct ataggtatag ctgaggcaaa
    18841 agaagcaggt aaatctataa tgttcttact agaaagtaac cctggaccag gtttaggagt
    18901 tctattagca tattggatgt ttagtagagg aagtgtaaaa caatcagcac caggagctgt
    18961 aataatacat ttctttggtg gtatccatga aatttacttc ccatatattt taatgaatcc
    19021 agttttaata ttggctacaa tggcaggtgg agcagcagga atattgactt tttcaatatt
    19081 aggagctggg cttgtagcag caccatcacc aggaagtata tttgcactta tggcactagc
    19141 tccaaaggga ggattacttc cagtattagc aggggttgca gtggcaacag ttgtatcatt
    19201 cttagtggca gcaccatttg ttaagagagc ttcagcaaat gaaagtgaag aagacagtac
    19261 ttctttagaa gaagctaaag caaaaatgag tgatatgaaa tctgcaagta agaactctga
    19321 gaaaaatatt gagaaaaaac agcttgaagt aaatgagatt aagaagattg tatttgcatg
    19381 tgatgcagga atgggttcga gtgcaatggg agcttctaga tttaaaaata gaataaaaaa
    19441 cttagattta gatatagaaa ttacaaattc atctgttgat aatcttccag atgatacaca
    19501 aatagtagta actcacaata cattagttga aagagttgct aagaacaatt caagtgttga
    19561 gatagtttca ataaacaatt ttttaaatga cccaaattta gatacattat ttaaaagatt
    19621 agaatctaaa taaaaataaa aagtaccact aattaggagt ggataatttg aaaaaaaaga
    19681 agattacatc taggcaaaaa aagataatac taatgatagt agaaaattca aagaaaaaca
    19741 ttccaataac tatttctgag attgcaggga cgttagagtt aagttcaaga actgtactta
    19801 gagatatgtc tggaattgaa aaatggtttg atgaaaatga ttttaacttt gtaaaaaaac
    19861 caggagtagg attgatttta gaggaaaata tagaaaatca aaactttata attgagttgt
    19921 tagaagaaga aaaaatagaa aaagaataca gcaaggaaga aagaaattta attatattat
    19981 caaagctact agtttctaac gagccagtaa aatcttatta ttttgcaaaa atattaaagg
    20041 tatctgaagg ggtactgaat aatgattttt cattagcaag taaatggttg gaaagatttg
    20101 atattgagct tgttagaaaa ccagggcttg gtgtctacct aaaaggtcaa gaaaaaagct
    20161 ttagagaggc atatgtaaat cttatatacg attcattcaa tgaaaaagaa atactagata
    20221 tggtcagaaa tataagtgaa aatatacaaa cagataaagc aatagaaatt ttaagtgaaa
    20281 atagactttt aaatttaatg gataggtgca taataataaa agttgaagct acattaacaa
    20341 agaagctttc agatttagat gtaaatttag ctgatagtgc atatataggg cttgtagttc
    20401 atatttcatt agccttacag aggataaaaa atggtgaaaa tataactatg gataaagagt
    20461 ttctaaaaga attatctata acagaagagt ttaaattagc taaagaaatc gtaaaaggta
    20521 tggaaacaga ttttaacatg gatataccag tagatgaggt tggatatata actatgcata
    20581 taagaggtgc aaagcaaagg tcatcttcaa atcataaggc tttaaacttg gatgatatag
    20641 aaataatgga aattacaaat aagatgatag atttggctga agatgaattt aaaataagtt
    20701 taaaaaatga cgagaggtta tttaaagacc tagcaaatca cttaggacct tctataaata
    20761 gattgaatat gggattagaa ataagaaatc ctcttttaga tgaaataaaa agcaagtatt
    20821 catatgccta tgatggagtt gaaaaaattt ctagaattat aaaagataaa ctaaatataa
    20881 attcaatacc tgaatcagaa ataggatata ttgcaatgca ctttgcatca gccatagaaa
    20941 aaaatcttat gatgaatact aatataaata ttgttgtggc atgtcccact ggaataggta
    21001 cttcaagatt cttatcaact aagattgaaa ataaatttcc taatttaaat atacttgaaa
    21061 ccatatcagc tataaacata gatgaagagt atttaaaggg aaaagatgtt gatttaatag
    21121 tgtctacagt tgagttaaat acaagtttaa attatatatg tgtaggtcca tttatgagtt
    21181 tagatgatga acagataatc aaggaaaaaa taaaatctat agctcaaaat aaattaatta
    21241 atttaaatac aaaaaatgat actaaaagta agaataaagt atatgaacag ataacagaaa
    21301 gtatgaatat aggaaaagat attcttcaat ttttagagga aattagattt gaaaaatttg
    21361 aaagtaaaga tttaagtgag cttattgaag actcatcaaa aatatttgca aaatctagtg
    21421 aagatataat gtctataaaa gaatcgttaa aagaaagatt aaaaatctca attccatata
    21481 tagaagaatc aaaaattcta ttattacatt gtatgagtga aagaatagat attatgaagt
    21541 tatcaataat aaaactggag aatcgaatcg tgctagattc aaatgaagaa atcgacaatg
    21601 tagtttttat gttacttcct aaaaattcac caagttatca aagacaaata atgagtgaaa
    21661 taagtggttc attaatagac aatataattt ttactaataa gataaataaa ttctctatag
    21721 aagagatgac attagaggta aaagatatag tttttaattt ttatacaaac agattaaaag
    21781 catttataga tgaataaaat taatattaaa aaggaaggtt gatactatga gtaaagtatt
    21841 gaatgagaat aatatatttt tagggttaga tagtgtttca aaggaagaag ctataacttt
    21901 agcaggaaga aaattagttg aaaatggata tgtaaaagaa gaatatatac cagcaatgct
    21961 agaaagagaa aaagtaatga ctacatatat gggaatggga gttgctattc cacatggtgt
    22021 aaatgaagca aaaaaagaaa tattatcttc tggaatagtt atactacaat tccctaatgg
    22081 tatagatttt gatggagaaa aagcatactt attaatagga atagctggtg ttggagatga
    22141 acatttagaa atactatcta atatagcagt agttttagat gatgacttaa cagaaagact
    22201 aaaaaattca aatgacaaac aagcttttat ggaagctttt gccaaataat cttaggaggg
    22261 atttaaaatg aaaaaggcaa tccagtttgg agcaggaaat attggaagag gttttattgg
    22321 aggcttgtta gttaagtcgg gttatcatgt agtatttgct gatgtaaatg aggagatatt
    22381 aaactcaata aataaagata aaaagtatac tatacacata agagatgtag aatgtataga
    22441 tgaagtaatt gataatataa gtgcggtttc atctataaaa gaagaaataa tagatgagat
    22501 tgtacaggct gaaataataa ctacagcagt tggaccttta gttttgacta aaatagcctc
    22561 tacaattgca aaaggtataa aggctagaaa agaaaagggt ttaacaagta atctaaatat
    22621 aatagcctgt gaaaatgcaa tatacgcaag ttcttcttta aaagaagagg tacttaagta
    22681 tttaaataag gaagaagtag aatatttaga gatatatgtt ggttttccaa actgttcagt
    22741 tgatagaata gttcctcctg gtaaaaatga aaatcctctt gatgtaacag ttgaaaattt
    22801 ttatgagtgg aatgttgaaa aacaaggatt taaaggggaa ataccaacta tagttggaat
    22861 gaatttagct gacaacctta tggcttatat agagcgtaaa ttgtttacac tgaatacagg
    22921 tcatgctata acagcttata taggatattt aaaaggatat aagactatag aagagagtat
    22981 aaaagataaa tttatctgtg atatagtcaa aagtgcaatg gtagaaagtg gagaaggatt
    23041 gattaaaaaa tacaattttg actcagaagc gcactataaa tatattgata aaattttaaa
    23101 tagatttaaa aatccatatc ttaatgatga tgtattaaga gttggaaggg aaccattaag
    23161 aaaactaagt gataaagata gattgattaa acctttaatg acagcaaaat catatggatt
    23221 atcagttgac aatttgattc ttggaatagg tgcagcgctt cattataata atagtgaaga
    23281 tactcaaagt gtagaattac aagagttgat aaagtctaat ggtgtaaaaa aagctgttgc
    23341 taaaatagct aatataagca atgacgaaga acttttaaat aatatcgaaa aaagttatat
    23401 atttatgaaa aatttataaa tttacttgaa aaaaagtagt tctgataaag taactataat
    23461 atattattct acacagtttg aattatttgt tgaataatag agaattataa tatataatta
    23521 aaaggaataa attaatatat ttacctgtat atgtttgata atatggttca aatgtttcta
    23581 ctaagctacc attaatagct tgactacagg agtgaagaag atatagttta tacttatttt
    23641 aaattatata ttaagttaat tactttgaat tttaagctcc tgtctatgat atagacagga
    23701 gcttttttag tattttaata taaaatactt acaagaatag atagcaaaat tagaacaaat
    23761 ataatttttt tattaatata actttagaaa gggctaggtg gaagttcaat gaaggcttta
    23821 caagaaaaga tattgagaga aggcagtgtt tcaggaaatg atatactaaa agttgatagt
    23881 tttttaaacc atcaaattga tgtagctttt ttaaatgaaa taggaagaga atttaaagag
    23941 agatttaaag aagaaaaggt ggataagata tttacaatag aggcttcagg aatagctata
    24001 gcatcaattg tttcacagta ttttgataat gcacctgtag tatttgcaaa aaaatcagaa
    24061 tctaaaaatt tggataaaga tgtatatgag actaatgtat attcatttac taaggcaaga
    24121 gaatattcag ttaaagtttc taaaaagtac ataaacaaag gtgaaaatat attgatagtg
    24181 gatgattttt tggcaaatgg aagagctgct cttggactta aagatttaat tgagcaagca
    24241 gaagctaatt tagttggagt aggaattgtc attgaaaaag gatttcaagc tggaggagca
    24301 cttttaaagg ctaatgatgt aagattagag tctttggcgg tagttgaatc tatagataat
    24361 ggaactgtaa aatttaggta atttatgata ttatatactt gtgtttagca catattttaa
    24421 ctatgtaatt atggaggatt ttttatgata ttttttatag acacagccaa tattgaagaa
    24481 ataaaagaag caaatgattt aggtgtaata tgtggtgtaa ctactaatcc atctctaata
    24541 gctaaagaag gtagagattt tatagaggta gttaaagaaa taagtgaaat agtagatgga
    24601 ccaataagtg cagaagtaat aagtttagag catgaaggta tgatagagga agcagaaaaa
    24661 ttatctaaga tacataaaaa tatagttata aagataccta tgacagcaga aggtctaaaa
    24721 gcggtgaaag tactatcaag taaaggtata aaaactaatg taacacttat attctctgct
    24781 ggacaagctc ttttagctgc tagagctgga gctacatatg taagtccttt tgttggtaga
    24841 ttggatgata tatcacaaaa tggtcttgat ttaattgaag aaatcgttga tatatttagt
    24901 gtaaatagta ttgaggctca aataatagtt gctagtgtta gaaatccaat acatgtttta
    24961 caagctgcta gaatgggagc agatatagca acagttcctc ttaaagttat aaaccaaatg
    25021 attaagcatc ctttgacaga caaaggaata gatagcttta tgaaggattg ggaaggtgct
    25081 tctttaaaat tataattata aactacgctt tataagtata ttaaagaggt tattatggaa
    25141 attctaagta ataaggctag gctattttgc ctagtcttat tttggtgtat aatatattga
    25201 aacatattat tgaaatagat taatttttta agtgaggagg cgaaaaatgg ataaaatatt
    25261 aagagagata caaagagaag ataaaaaaaa tccatatact gaccaagaac ttgctgaaat
    25321 gttaaatgta gctaggtctg aagtgatatc agtgaggaaa aaaaataata ttttagattc
    25381 gagagaaaga agaaaaaaaa tattaataaa agatatatca aaaattgtaa gtgaaaatcc
    25441 tcaaatgtct gaaagaaaaa taactgagat attaatagag aatggatata atatatcgag
    25501 aagtgcagtt tcaaaattat taaaagaaga aaatttaaat cagtcaaaga ttgggattaa
    25561 agataaagag ccagcatcat caggaaagta tgtgaaggtg agtaaagttg tagaagaaga
    25621 tggatttgaa gaattgatag gtataaaggg aagtttgaaa gaaaaggtaa atctagctaa
    25681 gtcagcaata atgtatcctc ctaatggcct tcatactata atatatggag aaacaggggt
    25741 aggtaaaagt gaattagcta catgtatgta taaatatgct ataaaaaaca acatcaaaga
    25801 agagaacagc ccatttatag tgtttaactg tgcagactat gcagaaaatc ctaatctatt
    25861 gatagctcaa ctctttgggg tagtaaaagg tgcatataca ggagctgatg caaatagaga
    25921 aggtcttgta gaacaagcta ataatggaat attgttttta gatgaaattc atagattgcc
    25981 tgctgctggg caagaaatct tattttcatt aatagataga ggagaattta gaaggttagg
    26041 agaaagtagt tctaatagaa aggcaaatgt tctcataata agtgctacaa cagaaaaccc
    26101 agattctaat ttacttcaaa catttagaag acgtatacca atggtaataa atttacctaa
    26161 tatgagtgaa agaccaaaat ctgaaagata tgaaataata ataaagtttt ttgaaagaga
    26221 agcaaagcga gtaaacaaga attttataat aactaaagaa gttatgtgtg cacttatgaa
    26281 ttataagtgt actggaaata taggtcagtt aaagagtgat atacaagtaa cttgtgctag
    26341 agcttttagt aaatcaatat tttctagcga taaggtaatt atagatttag atagtttaag
    26401 ggattatata aaaagtggat attatgattg taattgtaaa aatgaaaagt attctgagtt
    26461 ttcagttgag gatatatata tagatatcag tgaattggtg agtggaaaaa atagaatttt
    26521 atctgataat gagataagcg agatatataa ttatgcagaa aaagagctta aggttttgga
    26581 aagtaagtgt ttttcagaag aagagttaaa aaatgcattt atagaaaagt tagataagaa
    26641 gtttaatgct attaaaaaca ataaaaattt atctgataga agaagtcgaa tagactgggg
    26701 aggacaatta aaagagtcta cattggagat aatggataag gtaattgtat ttattaagga
    26761 gcagtttaag catgtaaatc aaggattgta cttggctcta gcaattcaca ttgagcatgc
    26821 tatagctagg ataaaagatg gaaaaactat aataaatccg agtttagata aaataaaagc
    26881 atcaatgcca atggaatatg aattgtcaag atatataatg ggtatagtgg aagatttgac
    26941 acaagtaaca tttccagaag atgaacttgg atatttggct tattatataa ataaattttg
    27001 ttataatgag gaaagtataa aagataaagt taaagtagta atagttacgc atggtaaagt
    27061 tggaattgaa atgtctaaag ttgtaaatca tattttaggt atagaatgta cactaggtat
    27121 agaaatagca cttacagatt caccatcaga ggggatagag catgtattag aagagcttca
    27181 aaagatagaa gctagaaaag gtattctagt tctaattgat atgggttctt tggtaattct
    27241 tggtgatgag gttgaaaaaa gacttggaat tagatgtaaa actgtaaaca gagtagatac
    27301 gttattggca atggaagcag gaaaacttgc aaccattgaa ggaaaaagtt tagatggtat
    27361 aatagcagat ttaaagaaaa ataagaatta tgccatgata aatacaaata aatttagtta
    27421 cagaaaaaac gagtatggaa agaaaaatgt aatcattact ttatgtttga gtggtgtagg
    27481 tacagcttta aatttaaaag agcatataga aaaacaaatt aaggaatatg acactttaat
    27541 tgaggtaaag ccagttgctt ttttaaataa taatttagaa gaagagttaa atgatattga
    27601 agctgaatat aatattattg ctatctgtgg aactatcgat atagattata aaaacgtacc
    27661 ttttatttct tataatgagg tacttggtaa aaatggtatt aataaagtat tagagcattt
    27721 aaacagtaaa caaccagttt taaatattga aaaccagttg aataatttaa tacatgaaga
    27781 tttgatttta tacaattttg aaggaatttc aaaggagtat ataattgata ctcttgtttc
    27841 aaagcttgaa gaaggtggat atgttgactc aaagtatata ttaagtgtat ataaaagaga
    27901 agcaatggga agtgttgtaa tggcctctaa ggtagccgtg ccacatggac ttccagaaaa
    27961 cgttataaag cctgcaatag caatcgcaag gttgaataag cctatagtgt gggacaataa
    28021 atttatggtg gatttggtag tactactggc acttaaagaa aataataaga aagaaatacg
    28081 aagcttattt tcaaagatta atgaccaaca tactttagaa gttttattaa atactgaaga
    28141 taaaaatgaa ataaaagaac ttttaagcta attattagta cagagaagaa agacaaaaaa
    28201 tttgctttag tataaaattt aaaactactt aattgctaag tagttaatta ctcgatataa
    28261 ggctagttct atgaaattta tttatatgat tttatagaat tagctttttt cttggtaaat
    28321 aaaatatatg aatttattta tgcaaaaagt aaattttatt ggacaattct atgagaattt
    28381 ttcctatatt tcttataaaa tatagaatat ataaaaataa attaaattca taataaataa
    28441 ttttataaaa tagttgttaa acataaaaaa ccatttgtaa aaaacaaaaa aatcatttct
    28501 ctaacaaaaa aaaccacaaa aaatataaaa aagtatggct agttaagtga ttaacaagta
    28561 aaaattaaaa taaaaatcaa aaaaaattta tcaatgaaag tcagctaaaa tcctggaaga
    28621 gagcattatt tttgggaaaa cattttcttt tgtttcacaa aaaaagccac tttttggcac
    28681 acaacatgct ttatataaag tcaagaaaaa ctaaggtata agacaaaggc aattaggagg
    28741 tgtaaatatg gagtttataa attttgtaag tgataaagtt atagacctta aaatggattg
    28801 taaaacgaaa gaagatttct ttaaagaaat acataataaa gtttttgatt taggctttgt
    28861 aaaagaagaa tttggagaaa aaatattagc aagagaaaat gtatttccaa caggtttaaa
    28921 tttaggagat tatggagtag caataccaca tacagatgca gaatatatta aagagcaatt
    28981 tatatcagtc tgtactttta atgaaccagt agtgtttagt tctatggaag accaagatga
    29041 aaaagtacct gtaaatttgg ctttcgtatt aggacttaat caaccacaca gtcaattatc
    29101 tgtattaaca gaattaatgg gaattatgca aaataaagag ttagttgata agttgatgag
    29161 ttctactgat aaagaagaag ttttaaagac actaaagtct ttataaaaat aaaaaagtac
    29221 taaaaataaa aaatataaaa ataaaaatta ttaggaggaa ataaaaatga aaaaaatatt
    29281 agtagcatgt ggagcaggaa tagcaacatc aacagtagtt tgtgacaagg tagagagatt
    29341 agttaaggaa aataatatac aagcagaagt tatacaatgc aaaatagctg aatgtgcaac
    29401 aaagcaagaa ggagcagatt taatagtatc aacaactata ctaccaacaa cttataatat
    29461 accagcaata aaagcaacag catatataac tggagtaaat acagcagcat tagataaaaa
    29521 aatattagat gctttaaaat aatcctatca tagatacatt gaaataatat tttataaaac
    29581 agaaaaagga ggatttataa atggataaat tattagcagt agtacaatac attttaaata
    29641 tgggaccgac agtaatacta ccaataacaa ttgtagtact aggaatgata tttagaatta
    29701 agtttacaaa agctttaaag tcaggtctta caattggtat aggatttgtg ggtataaact
    29761 tagttgtaaa cctattaaca tcaacactag ggccagcggc tcaaggaatg gttgaaagat
    29821 tcggacttaa tttaacagta atagatgttg gttggccaac aacagcatca gcaacatggg
    29881 cttcaccagt tgcaccaata ttaataccag tttgtttatt agtaaactta ctattattat
    29941 acttcaataa aacaaagata ttaaacgttg atatatggaa ttattggcac tttatagtag
    30001 caggtgcagt tggatatata gtaacaggaa gttatgtttg ggctatattc tgtgcaatac
    30061 taatggagtt attagtactg tttattgcag aaaagacagc aaaaaatgtt caggagtttt
    30121 atgatttaga aggtatagca ttaccaactg gttcaacagc ttcatttgct ccacttggat
    30181 atttagtagg taaatttgtt gaaaaaatac caggaataaa taaaatacaa gcagacccag
    30241 actctataca aaaaagattt ggtatatttg gagaaccaat gatgatgggg attatactag
    30301 gtataatact tggagcactt gcgggatatg atgtagcagg aatattcaat gtaggtatat
    30361 caatgggtgc agtaatgtta cttatgccta gaatggttag aatattaatg gaaggtttaa
    30421 ttccaatttc agaatcaata agagatttct tacaagcaaa atatggagac agagaacttt
    30481 atataggtct ggatgcagca gtagcaactg gacatccagc agttatatca acagctctta
    30541 tacttgttcc tataactttg ttcttagcag ttatactacc aggaaacaaa gttttaccat
    30601 ttggagactt agcgacaata ccattttata tggcatttat agtgtgtttt agaaaaggta
    30661 atatagttca atcagttata acaggaacaa tagtaatagc tatatcactt tatatggcta
    30721 caaactttgc agatgttcat acacaattat tacaacaagc aaatatgatg ccagaaggaa
    30781 caacaatggt tacaagtata gatacaggtg gaaacttgct aaaatgggga atattgaaat
    30841 tatctcaatt agtttcttca tttggaatat tctagtatgt agttaataga gattactctt
    30901 ttttagtaaa gtatgcgaaa atttaacatt tagcagctac taaaaaagag taatattttt
    30961 tataaatttt taaaaataca aaatacaaga ggaggagatt tatcgtgaaa gcagcagtat
    31021 tacatggcac aaatgatatg agatttgaag atatagaaat aaaaccatgt gaaagtgatg
    31081 aagtaaaaat aaaagttatg gcagcaggaa tatgtgggtc agatcctcca agagtattaa
    31141 aacattggaa atatccagtt ccagctatac caggacatga gttttctgga gtaattgctg
    31201 aagttggtaa ggatgtaaaa aatgtaaaag ttggagatag agtagttgca attcctttta
    31261 taccttgtaa tgaatgtgaa tattgtaaaa gaggattatt ttcattgtgt gatgaccatg
    31321 gaatgttagg agctaaaagt tttggagctt ttgcagagta tgtaaatata aaagctacaa
    31381 atgtacttcc aataggagat atgaactttg aagatgctgc aatgatagag ccactagcag
    31441 ttgctatgca tggagtactt aatataggtg ttcaagttgg agacactgta gctgtaatgg
    31501 gtagtggaac aatggggcag ttggtaatac aaggacttaa aatagcaggg gcaggaacaa
    31561 ttatagcagt tgatatatct gataataaat taagagaatc aaaggaatta ggggctgata
    31621 taataattaa tgctaaagat attaacccag tagaaaaaat aaaagaactt actggtggaa
    31681 aaggtgttga catagctctt gaatgtgcag gttcaaaaat aactcaagaa caatgtttac
    31741 ttataactaa aaagaaaagt aagataggat ttttagggat agcatattca gatataactt
    31801 tatcagaaga agcatttgaa aatatattta gaaaagaact agaattaaaa ggtttttgga
    31861 actcttattc tgcaccattt ccaggacaag aatggactaa gggaatcaat ctagttaatg
    31921 aaggaaagat aaaattaaaa gaaatggtat ctcatagatt ctcacttgaa gatacttaca
    31981 aagcatttga aatgataaga gatagaaaag aagaatttaa caaaatatta attttaccac
    32041 aaggggttga aaaataatga aagcagtagt taaaactaaa ccaggctatg ataatgtaga
    32101 agttttagaa gtagaagaac caaaagctac aggagacaag gttaaaataa aagtagaata
    32161 tagtggtata tgtggttcag atatacattc atttaaaggt gaatatgcaa atataaaagc
    32221 cccagtaaca ttaggtcatg aattttctgg aattgttgta gaagtaggag aagatgttaa
    32281 aaatataaaa gtaggggata gagttacatc agagactact tttgcaactt gtgagaaatg
    32341 tatatattgt gaaactaaag attataactt atgcccaact agaaaaggta ttggtactca
    32401 agtaaatgga agttttgctg agtatgtatt atcaagagaa gaaagcatac atgtacttcc
    32461 tgaaaatgta tctttattat cagcagccct tacagagcca ctagcttgtt gtgttcatgc
    32521 agctcttgaa aagactacta tagaaagttc tgatacagtt ttaataatag gaccaggacc
    32581 aattggttta ttattagctc aagttgttaa atctcaaggt gctacagtta taatgtctgg
    32641 tgttacaaaa gataaggaaa gattagaaat agctaaaaat cttggagtag atagaactgt
    32701 aaatgtaatg gaagaaagtt tggcagatgt agtgaatgaa atgactgatg gaaatggagt
    32761 aaacaaagga tttgattgtt ctgggtttat gccagcagtt aacgaagcct taagactgct
    32821 tagaaaaaaa ggtactttcg tacaagttgg aatctttgct aaaaaattaa atgaaatgga
    32881 ccaagaagca ataatccaaa gagagttaca atatatagga agcaggtctc aaaagccaag
    32941 ttcttggatt acagcattag atttaatggc aaatgggaaa gtaaatacag aagctttagt
    33001 tactagaact gttggtttag acgaatttaa agaaggtatt acagctttaa tgaacgggga
    33061 agaaataaaa gtagcagtaa aatcttaaaa aaacatttgt attattttaa aaaaaattgt
    33121 aaaatctact gtaggataaa aaaatgtaag gtcttaaaaa ataacaggag gaagaaagta
    33181 tgagcactct tagagatcat tctaataata ttagaaaaaa cataataaaa atggttgcag
    33241 aagctaaaag cggacatcca ggtggttcat tatcaatagc tgatatgttg acagtattat
    33301 attttgataa gatgaacgta tgtgcagaaa atccaaaaat ggctgataga gatagatttg
    33361 ttctatcaaa aggtcatgca gcaccagctt tatatgctac attagcagaa aaaggatttt
    33421 tcccagaaga agaattaata acactaagaa aatttggttc taagttacaa ggtcacccag
    33481 acatgaaaaa agtagcagga atcgatatgt ctactggttc tcttggtcaa gggctatctg
    33541 cagcaaatgg tatggcactt gcaggaaaat tagataacaa agactataca gtatatacta
    33601 tattaggtga tggagaaatc caagaaggtc aaatatggga agctgcaatg acagcatcac
    33661 attacaaatt agataattta attgcatttg ttgacttaaa tggattacaa atagatggtt
    33721 ctaatgaaga agtaatgaat gtaagtccag tagatgacaa attcaaatct tttggatgga
    33781 atgtaatagt aataaatgga cattcttttg aagaaatagg aaatgcagtt gatgaagcta
    33841 aaaaagttac aggaaaacca actgtaataa tagctaaaac agtaaaaggt aaaggtgttt
    33901 catttatgga aaataatgct gcatggcatg gaacagctcc aaatgcagaa caagcagagc
    33961 aagcattgaa agatttagaa ggaggtctat aggaatggga atagcaacta gagaagctta
    34021 cggacaagtg ttaaaagaac ttgcagaaaa taaagatata gtagtattag atgcagattt
    34081 aggaaaagct acaaaaagta tatcatttaa agaagttgcc ccagatagat tttttgacat
    34141 ggggatagca gaaggtgaca tgattggtac agcagcaggt cttgctactt gtggaaaaat
    34201 accttttgcg agtacttttg caatatttgc agcaggaaga ggctatgagc aaataagaaa
    34261 ctctgtagca tatccaaatt taaatgtaaa aatagcagct actcatgcag gtgtaacagt
    34321 aggtgaagat ggtggtagcc atcaagctat agaggatata tctctaatga gaggtatacc
    34381 aaatatggtt gtattaaatc cagcagatgc tttagaagca agacaagcta ttcttacatc
    34441 tattgattat aatggacctg tttatataag attaggtaga gctgcaactc ctgatgtaaa
    34501 cagtgaaaat tataaatttg aaataggaaa aggaactgtt ttaagagaag gttctgacat
    34561 aacagtaata gctacaggaa taatggtagc aaaagcctta gaagcagcag aagaacttgc
    34621 taaagaaggt ataaatgtag aagttgttaa tatatctaca ataaaaccat tagatgaagc
    34681 attaataaaa gaaactgcta agaaaactgg aaaagtagtt acagcagagg agcacagtat
    34741 aataggtggt ttaggttcag ctgtatgtga agctttagca gaaactaaag atgtagtagt
    34801 tagaagaatt ggtgttaaag atgtatttgg tcaatcaggt acaccagctg atttattaaa
    34861 acactatggt ttaactacag aagatatagt aaaaaatata aaagagttgt tatagatata
    34921 aatatatcta aataacaaat tgatttgaca taagtcaatt aaagctgtct taaaaataga
    34981 aagaaatttc tacaatggga cagctttatt tttacaatac tttattttta tgataattat
    35041 aaaatagtct gaaagtaatc aaaaaaatta taaaaagagg tgctatttaa tgaaaatatc
    35101 tattggatgt gaccatggtg gttatgaatt aaaggaatat ataaaggaaa atttaataaa
    35161 taaaggtata gaagttgaag atgtgggtac aaattcaaaa gaaagtgtag attttccaat
    35221 atatgcaaaa aaggtaagtc aaaatgttca aagtaaaaaa tctgatttag gcatactttg
    35281 ttgtggaaca ggaataggta tgtctattgc agcaaataag tttaaaggaa tcagagcagc
    35341 tgttgtttct gactgttttt cagctaaggc aactagagaa cacaataata caaatgtact
    35401 ttgtttagga gaaagagtta ttgggaaagg attagcaatg agaattgttg aagaatggtt
    35461 aaattcaagt tataatggag aaagacatgc aagaagatta caaatgattg atgatataga
    35521 aaaagaaaat ttataaatat ataatttaca acaaggggga gcgttatgtc tattatttgt
    35581 gcttcaataa tgtgtgcaga ccaaatgaag ttaaaagagg aattagaagc tcttgaagaa
    35641 gcaggtgtaa aattacttca ttgtgatgtt atggatggaa tatttgttaa aaacttggct
    35701 atgggaccag agttattaaa atcaataagt gaaaatacaa caataccttt agacatacac
    35761 ttagccacag aaactccaga taaatatatt gatatgatgt cttatataaa gccaaaatat
    35821 atttcttttc atgtagaatc aagtacaaat gtaaaagctg atatacaaaa gttaagaaat
    35881 tatggtatag ggccagtgtt agcaataagc cctcaaacaa gtgtggataa aatagaagaa
    35941 tatataagtt tagttgaggg tatcttagta atgacagtaa atccagggtt tgcaggtcaa
    36001 aagtttaatc tttctgtatt ggataagttg gataaactta cagagatact aaaagattat
    36061 gataatcctc catttataga ggttgatgga aatataaata aggatactat taaacttatg
    36121 aatggtaaaa aagtagatat atatgttgtt ggtacttcag ctttatttaa tgataagcct
    36181 ccaatttcat ataaagataa aatagaagaa ctaaaagaaa gtataaagta accatgtaat
    36241 aaaattataa tatgtttctg tttaatagta aaaatttagg tggaaacaag aaattttata
    36301 atatttaata aaaattttgg ttgagtaagg agaatttttt atgggagacc tatataaact
    36361 acaaaatggt actgatatta gaggaatagc gtatgaaaat gattctaaag aagttaacct
    36421 aactgtagaa gaagtaaaaa aaatagctaa agcgtttcat atttggttaa aagaaaagac
    36481 taagaaagat aaagtaactg ttgccatagg aactgattct aggataacag gaagccaatt
    36541 tagaagtact gttattgaaa ctttaactaa tgatgattgt aatgtaattg attgtgagat
    36601 tgccacaact ccagctatgt ttatgacaac tattatggat ggatacaatt ctgatggggc
    36661 gattatgatt acagcaagtc acttacctta ttactataat ggtttaaagt ttttcacaga
    36721 aagtggaggt ttagaaaaaa ctgatataaa agagatgttg gatatagctg ttaaaaatga
    36781 tagtgtagac tatgaggaag taaataaaaa aggtgaagtt atagtaaaaa atttaataga
    36841 agattattct aatttattga tagataaaat tagaaaaggt gtaaattcaa gtaaaaatta
    36901 tgaaaagcct ttttctgggc ttaagatatt ggttgatgca ggaaatgggg ctggtggatt
    36961 ctttgctgaa aaagttttac acatactggg agcagatacg acaggtagtc agtttttgaa
    37021 cccagatggt atgttcccaa atcatatacc taatcccgaa aacaaagaag caatggaaag
    37081 catatgtaaa gctgtgttgg ataataagag cgacctagga ataatctttg atacagatgt
    37141 agacagagct gctattgtag gtaaaaatgg caaaccaata aataaaaatg ctcttatagc
    37201 tgttatatca tcaatagtat tagaagaaca tccaaaaaca gctatagtaa cagattcgat
    37261 tacttctgaa ggacttgcta aatttataaa tgaattacaa ggaagacatc atagatttaa
    37321 aagaggatat aaaaatgtaa taaatgaagc tataagatta aatagtgaag gtgaagagtg
    37381 ccatttagct atagaaacgt ctggacatgc tgctttaaaa gaaaattatt tcttagatga
    37441 tggggcatat ttaatagcta aaattttaat aaaagtagca aagctatcct tggaaggaaa
    37501 gactatagaa gaattgatag aaaaccttga agaagctcaa gaagagaaag aagttagaat
    37561 aggcattaat aaagaagatt ttagaacata tgcagaggat atattaaaag atttagattc
    37621 tcatgttaag agtattgatg gatggtctgt agcaccaaat aattttgaag gcataagagt
    37681 taattgtgat gaatcaaatg gagatggatg gtttttactt agaatatcac tacatgaacc
    37741 attattagct ttaaatattg aatcaaataa aattggcgga gcagaacaaa tttattctaa
    37801 gttaaaactt ttcttagaaa aatatgattt aaaaggtctt tagggtaaat agaaagaata
    37861 tatttatatt gaagaataaa tgtaaatttg tacctaagat tacaaatatt acaaaattgt
    37921 aatcttaggt aacagagatg taactgatta tggaaacact atgtgatatt atagatacag
    37981 agagatgaaa tatgtgatga attatagaga aatataattt aaaatgtatt aatatcacac
    38041 caaaaaggta taatcaaaaa atatgtaaat ataaacccca acatttagaa aaagacctct
    38101 aaggaggtt
//
LOCUS       DACULQ010000016        38082 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.016, whole genome shotgun sequence.
ACCESSION   DACULQ010000016 DACULQ010000000
VERSION     DACULQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38082
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.016"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(432..1586)
                     /locus_tag="JA154_03505"
     CDS             complement(432..1586)
                     /locus_tag="JA154_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAT7994924.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSVELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYIPALSCNIDNVCGSIHQGMEADFIVTDKDFNIYETYISGICKYKVNK"
     gene            complement(1669..2394)
                     /locus_tag="JA154_03510"
     CDS             complement(1669..2394)
                     /locus_tag="JA154_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7994925.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            2661..3590
                     /locus_tag="JA154_03515"
     CDS             2661..3590
                     /locus_tag="JA154_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAT7994926.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVSK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYYVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            complement(3719..4636)
                     /locus_tag="JA154_03520"
     CDS             complement(3719..4636)
                     /locus_tag="JA154_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAT7994927.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMQFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAISGEECIKAQRLLEKIINSVK"
     gene            5112..6275
                     /gene="agaS"
                     /locus_tag="JA154_03525"
     CDS             5112..6275
                     /gene="agaS"
                     /locus_tag="JA154_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate ketose isomerase"
                     /protein_id="HAT7994928.1"
                     /translation="MFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKLSR
                     FIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNPYD
                     YLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKNDEN
                     NYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIVEMGRNVIDRK
                     DEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPKSF
                     IDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFKEK
                     YNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            6324..7175
                     /gene="kbaY"
                     /locus_tag="JA154_03530"
                     /gene_synonym="agaY"
     CDS             6324..7175
                     /gene="kbaY"
                     /locus_tag="JA154_03530"
                     /gene_synonym="agaY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089968.1"
                     /note="catalyzes the reversible reaction of
                     dihydroxyacetone phosphate with glyceraldehyde 3-phosphate
                     to produce tagatose 1,6-bisphosphate; in enteric bacteria
                     there are two D-tagatose 1,6-bisphosphate-specific
                     aldolases: KbaY (also called AgaY), involved in catabolism
                     of N-acetyl-galactosamine and D-galactosamine, and GatY
                     which is part of the galactitol catabolism pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HAT7994929.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            7501..9402
                     /locus_tag="JA154_03535"
     CDS             7501..9402
                     /locus_tag="JA154_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HAT7994930.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            9405..10448
                     /locus_tag="JA154_03540"
     CDS             9405..10448
                     /locus_tag="JA154_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M42"
                     /protein_id="HAT7994931.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            10463..10768
                     /locus_tag="JA154_03545"
     CDS             10463..10768
                     /locus_tag="JA154_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAT7994932.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            10865..11200
                     /locus_tag="JA154_03550"
     CDS             10865..11200
                     /locus_tag="JA154_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT7994933.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            11232..12554
                     /locus_tag="JA154_03555"
     CDS             11232..12554
                     /locus_tag="JA154_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT7994934.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            12564..13634
                     /locus_tag="JA154_03560"
     CDS             12564..13634
                     /locus_tag="JA154_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAT7994935.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            13639..14826
                     /locus_tag="JA154_03565"
     CDS             13639..14826
                     /locus_tag="JA154_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAT7994936.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(15023..15895)
                     /locus_tag="JA154_03570"
     CDS             complement(15023..15895)
                     /locus_tag="JA154_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAT7994937.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            16129..16248
                     /locus_tag="JA154_03575"
     CDS             16129..16248
                     /locus_tag="JA154_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994938.1"
                     /translation="MEKPNTKVVNFQEYKDRKNQREVNRDELLKLIKKIVTSK"
     gene            complement(16289..16480)
                     /locus_tag="JA154_03580"
     CDS             complement(16289..16480)
                     /locus_tag="JA154_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994939.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            16700..17746
                     /gene="cobT"
                     /locus_tag="JA154_03585"
     CDS             16700..17746
                     /gene="cobT"
                     /locus_tag="JA154_03585"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089959.1"
                     /note="catalyzes the synthesis of
                     alpha-ribazole-5'-phosphate from nicotinate mononucleotide
                     and 5,6-dimethylbenzimidazole; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAT7994940.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            17778..18338
                     /locus_tag="JA154_03590"
     CDS             17778..18338
                     /locus_tag="JA154_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAT7994941.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            18348..19121
                     /locus_tag="JA154_03595"
     CDS             18348..19121
                     /locus_tag="JA154_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAT7994942.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            19141..19755
                     /locus_tag="JA154_03600"
     CDS             19141..19755
                     /locus_tag="JA154_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAT7994943.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      19822..20001
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            20116..21630
                     /locus_tag="JA154_03605"
     CDS             20116..21630
                     /locus_tag="JA154_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HAT7994944.1"
                     /translation="MGKKIMLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMAL
                     NSFITKEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSS
                     KYHDYKLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPV
                     IIVGDIDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKV
                     PVIGVVPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVS
                     IRYVDYGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQ
                     MLGKKLKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGK
                     KVKGYEIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEIS
                     FTRAILNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            21669..23045
                     /locus_tag="JA154_03610"
     CDS             21669..23045
                     /locus_tag="JA154_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HAT7994945.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            23046..24017
                     /locus_tag="JA154_03615"
     CDS             23046..24017
                     /locus_tag="JA154_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase"
                     /protein_id="HAT7994946.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            24049..25119
                     /locus_tag="JA154_03620"
     CDS             24049..25119
                     /locus_tag="JA154_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase"
                     /protein_id="HAT7994947.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEEREYML
                     QELRNIRNIKVYDTDANFILIKIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            25121..26008
                     /locus_tag="JA154_03625"
     CDS             25121..26008
                     /locus_tag="JA154_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAT7994948.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            25996..26628
                     /gene="cbiC"
                     /locus_tag="JA154_03630"
     CDS             25996..26628
                     /gene="cbiC"
                     /locus_tag="JA154_03630"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089950.1"
                     /note="catalyzes the interconversion of precorrin-8X and
                     cobyrinic acid; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HAT7994949.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            26637..27863
                     /locus_tag="JA154_03635"
     CDS             26637..27863
                     /locus_tag="JA154_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HAT7994950.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDDGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            27860..28468
                     /locus_tag="JA154_03640"
     CDS             27860..28468
                     /locus_tag="JA154_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089948.1"
                     /note="catalyzes the methylation of C-5 in
                     cobalt-precorrin-6Y to form cobalt-precorrin-7W-a; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HAT7994951.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            28458..29033
                     /locus_tag="JA154_03645"
     CDS             28458..29033
                     /locus_tag="JA154_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089947.1"
                     /note="catalyzes the methylation of either C-15 or C-5 in
                     cobalt-precorrin-6Y to form cobalt-precorrin-7W;
                     decarboxylating; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6Y C(15)-methyltransferase"
                     /protein_id="HAT7994952.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            29026..29778
                     /gene="cbiF"
                     /locus_tag="JA154_03650"
     CDS             29026..29778
                     /gene="cbiF"
                     /locus_tag="JA154_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089946.1"
                     /note="catalyzes the formation of cobalt-precorrin-5 from
                     cobalt-precorrin-4; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 C(11)-methyltransferase"
                     /protein_id="HAT7994953.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            29762..30883
                     /locus_tag="JA154_03655"
     CDS             29762..30883
                     /locus_tag="JA154_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HAT7994954.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYHVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            30880..31605
                     /gene="cbiH"
                     /locus_tag="JA154_03660"
     CDS             30880..31605
                     /gene="cbiH"
                     /locus_tag="JA154_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089944.1"
                     /note="catalyzes the formation of cobalt-precorrin-4 from
                     cobalt-precorrin-3B; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-3B C(17)-methyltransferase"
                     /protein_id="HAT7994955.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            31602..32354
                     /locus_tag="JA154_03665"
     CDS             31602..32354
                     /locus_tag="JA154_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HAT7994956.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            32373..33851
                     /locus_tag="JA154_03670"
     CDS             32373..33851
                     /locus_tag="JA154_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HAT7994957.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            33881..34786
                     /locus_tag="JA154_03675"
     CDS             33881..34786
                     /locus_tag="JA154_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAT7994958.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSAIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            34776..36275
                     /locus_tag="JA154_03680"
     CDS             34776..36275
                     /locus_tag="JA154_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAT7994959.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            36292..37257
                     /locus_tag="JA154_03685"
     CDS             36292..37257
                     /locus_tag="JA154_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAT7994960.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            37410..37988
                     /locus_tag="JA154_03690"
     CDS             37410..37988
                     /locus_tag="JA154_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HAT7994961.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        15173 a         4011 c         6754 g        12144 t
ORIGIN      
        1 aacttttaga tatatacaat aagtgctttc atttgtaaaa atttctatag tattatcttg
       61 tttatttaca cctacaatat ctttaaaatt gatatgattt ttagtatttt tacatttgaa
      121 aatatatctg ttatcaatta aaatctcatc attagtaatt gaaatatagg gtttatctca
      181 tgctttatta aaaatttata tgtttgttca agataaaaac ttacaaataa taatagaaaa
      241 gttactttaa atgtacatat tatcgaaaat attatcacaa aaatacttat aatatatatt
      301 tgtttaaaat aacctctatc gcaatagttt attaacgata tggaaataaa agtgttaaat
      361 cagattttgg agaaataact ttaaatattt ccagactctt tttagacagc ctccataacc
      421 ttatattgaa cctatttatt aactttgtac ttacatatac cacttatata agtttcgtat
      481 atattaaagt ccttatctgt tactataaaa tctgcctcca taccttgatg tatggagcca
      541 caaacattat caatattaca acttaaagca gggatatatg tagccatttt tattgcatct
      601 tctaaagtgg ctatgttcca atcaactata tttctcacag ccttgtccat agttaataca
      661 cttccagcta aactcccact atttaatctt gcaacactat tttttacatt tacattaaac
      721 ttaccaagag tatattttcc atcctccata gcaccagctc tcatacaatc tgtaattaaa
      781 gctatgttat tactagactt agcatccatc attattttaa cagctattgg gcttacatga
      841 tgaccatcac aaattaattc agcataagta tcttttagta ccatagcagc cccaaccatt
      901 ccaggttctc tatgatttaa aggactcatc ccattatatg tatgtacaaa cactttagca
      961 cctgaattaa cgacttgttt aacttgctca aatgtagctg aactatgacc aagagaaact
     1021 gatatattat ttttagttgc atatcttgtt aattctactg aacctttacg ttctggagca
     1081 attgctattt ttcttattaa atttttcgat tttttttgcc ataattttaa tatattgatg
     1141 tcagggtctt taaaatattt ttcattttga gcgcccttat atttttctgt aaaaaatgga
     1201 ccttctaaat aaatcccttg tatttttgca ccctcaactc gattgaagct attgcttata
     1261 ttttctacag aatcacttag tgtatctaca gaatcagtta aagttgtagg taggaaagat
     1321 gttactccat attttaataa gccttttgaa attgtgttta ttgcttcaaa tgtattgtcc
     1381 atgacatctg cacctaaaag accgtgaatg tgagtatcaa ctaagccagg agcaatatat
     1441 ttacctgaaa aatctataac attatcagta acattaggtc tttcttgttg aaattcaccg
     1501 aatttgccat taattatact aaggtaacct ttttctttga ttccatgttt cataaaaaat
     1561 ttatctgcat aaataaaact tttcaaaata acctccttat aattatatta tcattgtggc
     1621 attgacgctt ataatatcat tctgttttct tgaaagttat ttaacttact atcttacatg
     1681 tcttactcta tatacaaatt tatcacttct agcgacactc aaagtaaatt caataactat
     1741 attgtcatca ttatatgtgg tacgaagtaa tttcagacaa ggtgaatctc ctggtacttc
     1801 caataactta gcttccttat ccgagattat tccagctgaa aattcttcat cagcaacttt
     1861 aataatctcg ccataatctt ctctaaatat ttcatataaa ggtttttgtt cgattttttc
     1921 ttttgtcaat cctataaatt ctttcatagg aagatatgtt ctttcaagca tcataggaag
     1981 gtcatcagca agtcttaaac gcattatttt tatcattttt tcaccaggag ttatacctaa
     2041 tttttcagag aaatactttg tactttctac tacctcaaat gataatacct ttgtacctgg
     2101 cttttttcct tgtgttctca tataatctgt aaaactataa ctttccatta agttttgaga
     2161 attttgactt agtgcagcaa caaaagttcc ttttccatgt cttttgtata tatatcccat
     2221 atgctctaac tcagccatag ctagtctaac tgtggttcta cttactccat attcatcaca
     2281 aatttttctt tcggaaagca ttctatcgtt tggttccatc tcattttcaa tcttagttct
     2341 taatatctca acaagctggt catatattgg tcttttatca tttcttttta tcataaaaat
     2401 atagtccttt ctaagtggtt attaccacaa tttgattata gaatatagaa ttatttatgt
     2461 caatgaataa agtttaatca aaattcaata agtaaggcta ttaatttatt ataaaattat
     2521 ggtacattga caaaacaagt aaaataattt atattcaaat tggtaataac cactttggat
     2581 atattatatg aggtattttt tattttacag attaatttat tgtttagaaa agtaaaaatt
     2641 aataatgagg agaatctagt atgattacag taataacatt caacccatct atagatagaa
     2701 tgtatagagt gaataatata aacataggtg aggtgcaaag ggtagtaagt aagaatgcaa
     2761 ctgctggagg taaaggatta aacgtatcta aggtatgtaa aattttaaaa gaaaatcctt
     2821 tagctatggg atttctaggc ggatttaatg gagaatttat aaaaaatgaa cttagaaaac
     2881 tagatataca aaataaattt acaaaaatag agcaggaaac tagaaattgc ttaaacataa
     2941 ttgaggacaa taaagttagt acagaatttt tagaaaaagg accaatagta gaaaaaagcg
     3001 atttagagag atttgaaaat gatatagagg aagtactaaa aaatactaaa atactggtag
     3061 cttctggtag ttattgtgag aatatgccga tagactatta tgaaaagata ggaaatatat
     3121 gtagagcaaa taatataaag tttatattag atacatcagg agaagcatta aaaatagctt
     3181 taaaatcaaa accatattta ataaagccaa atatagatga aataagacat ttattaggaa
     3241 taaatataga atcaagagag gaaattatct tgtcaggaaa aaaacttatt gaaatgggtg
     3301 cagagaatgt atgtatatct ttaggaaaag atggtatggt gtatttaaac gaagataacg
     3361 tatattatgt aaaagtacca gttgtaaagt gtataaatac tgtaggtagt ggagatagca
     3421 cagtggctgg atttagtgta ggtattttaa ggggttataa aatagaaaat ttacttaaat
     3481 ttgcaaatgc ttgtggtata agtaatgctt taaatatgga aactggattt gtagatttgg
     3541 gagaaattga taaatatcaa gatttagttg aagtaaaaaa gttaagttaa atacatttaa
     3601 tgggaatata ataaatttaa tgttgttgta atagatttta attttaatat aaaagatata
     3661 gtcttatata aaaataggtt agaaaaattc atttttaacc tatttttata ataaatatct
     3721 attttactga atttataatt ttctctagta atctttgagc cttgatacac tcttctccac
     3781 tgatagctgg ttttatatta ttttctatac aatcaataaa atgatttgat atatcctcaa
     3841 aacccttttg ttttaatata tttacccatg caccactttg ctttaaagtt aggtttccac
     3901 cttcttctat ttccagtaca ttaagattct taactctttg aatcttaccc tttgaaagaa
     3961 tttcaacttg ttctaacttt gttccagaat ctctatgcat agaggtgttt atagagaaat
     4021 ttttaccttt aagtttatga cttacaaata taagattctt attgtctgtt aaaaataaat
     4081 cctctccact aatttcaaca tcttcatttg ctaaccataa agcagtgtca ataacatgta
     4141 tatagtcatc tataagagta ctgtcgaatt taacatttct taaggaattt aatccatgtt
     4201 tacatatatt gatagataca atttctgtag cattattttt aatttctttg tacataggac
     4261 agaatctacg gttgaatcca accattaaat ttaagttttt tttcgtactt aattcgataa
     4321 gttcttctcc ttgacttact gttgaagcaa gaggtttgtc tacatataca tgaacaccta
     4381 aatttaataa tatttttata atttcgtagt gtgtttctgt agagctatgt agaaaaatac
     4441 aatcacattt tttagctagg gattcaatag aatcgaattg catgattcta taatcactac
     4501 agactttttc ccttttaact ttatttggag taaaagctcc tacaaactca aatctctcag
     4561 acttagttaa tataggtaaa tatgcctttt gagctatgga gccaagacct atcattccca
     4621 tttttatatt tttcataata ctttctccta aggtttttat atttacagaa ttgattataa
     4681 ttcagacaca taaatttgta aactttgtat caaaaaataa atttaaagct tatataagta
     4741 aattagcgaa ataagaaata ttgataaagt aattttgtag gatatctata aaattgattg
     4801 atataaatat tttatgtatt gacaaaaaat tcaaattaat ttatatttgg attagtggta
     4861 attaccactt tggtctgaac aagtttttat cgtaaaataa aaatactaaa gtatatagag
     4921 tatgatttta ttaaattttt aaagataaac tataaaatta attaaaatct atagtgtata
     4981 ttttatagtg agtttaagta gtactaaaaa tctaagcaaa aaattataaa agagtatatt
     5041 tttttatagt ttaaagtggt tataacctca ttaccaaatg taaacgttac aagtatgaaa
     5101 ggagaatgaa aatgtttaaa ttagaagaac aaaaactaaa agatttaggt gcaataataa
     5161 caactaatga aataaaacaa cagcctgaat tatggttaga gacttatgaa atatataaat
     5221 ctaacaaaga aaaattaagt agattcatag atacaataag taataatcat gggcagttta
     5281 gagttatatt tacaggtgct ggaacttcag cttacatagg aaattcaata cttccttacc
     5341 ttaaaaataa aaatgacata agaaaataca tatttgaagc aatacctact acggatatag
     5401 tttcaaaccc atatgattac ttaaaaaaag atataccaac attattaatt tcatttgcaa
     5461 gaagtggaaa tagtcctgaa agtttggctg ctttaaattt agggaataag attgtagata
     5521 atttttatca tttagctata acttgtaatc cagaaggaga actagctaag atgactaaaa
     5581 acgacgaaaa taattacttg ttattaatgc catcaaaatc aaatgatgaa ggctttgcca
     5641 tgacaggaag tttttcgtgt atgatgttat ctgctatgtt aatattcgat agtttagaag
     5701 atgatgtaga aaagtcatat ataaatgcaa tagttgaaat gggaagaaat gttatagata
     5761 gaaaagatga aatacatgag ttaataaata aagattttga tagagttgta tatttaggtt
     5821 caggaggatt aggtggtttg acacaagaag cacaactaaa acttttagaa cttacagcag
     5881 gaaaaatatc tacagtgtat gattctccta tggggtttag acacggacca aaatcattta
     5941 ttgatgaaaa tacacttgta tttgaatttg tatctaactg tttatataca agaaaatatg
     6001 atttagatgt tttagaggag ataaaaaggg ataaaattgc taaattcact tgtgcagtgt
     6061 cagtagaaaa tgaaaataac ttctcaggaa caaagtttga gtttaaagaa aagtataata
     6121 aattaccaga tgtgtatctt gcaatgccat acatattgtt tgcacaaact atagcattgt
     6181 ttgtatcggt aaaggttgga aataagccag atacgccatc agctacagga acagtaaatc
     6241 gagtagtaaa aggtgtaaca atatatgaat attaaaagat aagattaaaa tattataact
     6301 aaataatttg ggagggtatc aatgtggcat tagtaacaac aacatcaatg cttaaaaagg
     6361 cacaagatga agggtatgca gtaggagcat ttaatgtaga aaatatggaa atggtaattg
     6421 ctgttataga agctgctgag gagttaaatt ctccagtaat attacaaact actccatcta
     6481 ctgtaaaata tgcaggtctt gattattatc tagcaaatgc aaaggttgct gcagaaagag
     6541 taaagatacc tgtagcaatg catctggacc atggttcaag ttttgacctt gcaatgaaag
     6601 ctttaagaag tggatataca tcaataatga tagatggttc tcatgaaagc tttgaaaata
     6661 atatatctat atctaaatca gtagtaaaag catgtagtcc ttctcttata ccagtggaag
     6721 cagaacttgg gaaagttggt ggaaaagaag atgatttaga tggcgggaat agtaatgatt
     6781 atacaaatcc aaaagaggca aaagagtttg tcgatagaac aggaatatct tcattggcta
     6841 ttgctatagg tactgcacat ggattgtata agggtgaacc taaaatagat ttagataggt
     6901 tatcagaaat taaaaatgta gtttcaatac ctttagtatt gcatggaggt tcaggaatac
     6961 atgatgatat tataaaagaa gctataaaaa gaggtattgc aaaagttaac tatgcaactg
     7021 aattaagaat tgcatatagt aaaggggtta aaaaagtttt agatgaagat tctgaggtta
     7081 tagaccctaa aaagtatgga ttagcagggc ttcaatcagt aaaggatttt gtgaaagaaa
     7141 agattaaagt gtgtgggtct gtaaatagag tataaaaata aaatatccta aatttatttt
     7201 agataagttt aggatatttg ttatatgaaa aatttatatt aattatatgt aaatggtgta
     7261 tttttttcat ttaattgatg aaaaatatca gcttttattg gtaaagtatt acatatataa
     7321 ttaaaatata ctaaaaattg aactatatat tttattatta taaaatattt atattaaatt
     7381 gtggaattaa aatatactag gatataaaat tcttaaataa aaagaaaaga ttgtttttat
     7441 aaaaaatatt ttttatacca cttaaaaaaa cacaataata acactgaggg aggtggggat
     7501 atgagagaac gcattattct tgaaacatta aaaaatagta gtggttatct aaacatagat
     7561 tattttgcag ataagttggg ggtatctact cgaactatca gaaatgaaat aaaaaagata
     7621 gaaactataa aagaatgtaa tggttttaaa ttagaatata agtctaaatt aggatatatt
     7681 ctaaatatta aagaccaaga taaattcgag cattatctaa gaaacttacc ctcagattta
     7741 gttgaaaatc cagaacaacg gttagagtct ataatagtag aacttttagt aaatgaaggt
     7801 tataaaacta tagaacagtt aagtaaaaaa tttctagtaa gttcatccca aataaaaaat
     7861 gatttaaaaa agatagatga aaagataaag gatacagaat taaaactaga gcgaaaggct
     7921 cattatggaa ttaaaataga aggttctata aaagaaattc aaagtatatt ggtggatagt
     7981 tattttagag ggaatagaaa tataacagag tacaggaata agtttataga taatttaaaa
     8041 ttagctaata ttagaagtac aataaaaaat gtacttaatg aacatgattt agaagcaaat
     8101 ttaacagaac ttgaggaaat attagcacaa attattatat tgtatataag agttagtatg
     8161 agagtagcgg gaaatgctag cgatttaaaa ttatatacag aagatttaat tatagataaa
     8221 ttattagata aaatttttag agataaaaaa tataacctta attatgatga aaagtattat
     8281 ttaaaggagc ttataaaact aaagactaaa gataagaaag ccactataaa gaatattgat
     8341 aagaataaac ttcaagatat tatgttcgaa ttttttagag agatagataa aaaatataac
     8401 tctaattttt tagaagataa agagttcttt aacttatttt acttacatat tgcatgttta
     8461 atagaacgta taaagaagaa ccacaagata ataaatcctt tttcagttaa aataagtcaa
     8521 cagtatccaa ctgtttttaa tttatctatt caattttcaa aaatcataga aaatgagtat
     8581 catataaaaa taagtcaaga tgaaataggg tttatagcta ctcacattgc tgttcctttt
     8641 gaaaagagag aagaagctaa ttttaataaa aaatataaaa tagcaataat atgctcttct
     8701 ggtggaggta gtgcattttt gataaattta agacttaaag aaatttttcc aaatgctgag
     8761 ataagaaatt tttcattact agaagaaaaa gctgtaatag aatttgcacc agatttgatt
     8821 ttttcaataa caaatctttt atttgaaata aatgctccag ttatacttat aaatgaaatt
     8881 ttagacgaat tggattattt agatattaaa gaaagtgtta gatttgcaga ccatatagga
     8941 aatatatcaa gcccaaaaca atatatatta ggtctatttg ataagaatca ctttaggtgt
     9001 ataaaagata aagctaagta taaggatatc cttgatagta tgtcaaaatg tatagttgac
     9061 gagggcgctt gttctccaac ctatcccaaa gatgtatggg aaagagaaag ctatttgagt
     9121 actatttata caaatggggt ggctatacct catccaatcg agatgacagg aaacaaaaat
     9181 ataatatcag tagctttgat acaaactgat attatttatg aaaatagggt gcctaaaatt
     9241 attttcatga tttcattaat aaagggaaat ttagaacttc ataagcaaat atctaaatat
     9301 ttaagtaaga ttatggcaaa taaggatatg gtagacatgc taaataagtc acaatcttat
     9361 gaagagttta tgtataagtt aaaaatctat ctgggaggtt agacatgaat atagagtttt
     9421 ttaagaggtt gtcagaagca gatggtatag catctaatga agaagaggtt agacatgttt
     9481 tattggaaga actaaaagct tatagtgata aaattatctg tgatgggcta gggagtatca
     9541 tcttttctaa aataaaagat gagagtgcac caaacgtaat gatttgtgct catatggatg
     9601 aagttggatt tatggtgaga agtatagaca aacttggtat gattcactta ataacaatag
     9661 gaggagtaaa acctcttgca cagtttgtac aaaaagttag aattacaaca aaagaaggga
     9721 agaaaatccc tgctgtcata aatgctacat ataataatgg taaggctgaa aatatatatg
     9781 cagatattgg agcatataca gaagaagatg tatataacct agggattaat gttggggata
     9841 tggtaacata tacaacaagc tttgaggaat ttactttacc agaccgatta gtgggaaagg
     9901 cttttgatga taggatagga tgttttgtga tgggagaagt tttaaaagaa cttaggaaag
     9961 aaaatctaaa ttgtaatatt cactttgcgg ctacaagtag tgaagaggtt ggaataaggg
    10021 gagcaaagac atcaacacag ttaataaatc cagatatagt atttgtaata gatgtagcat
    10081 gtgctaaaaa tgagtttgtg agagactata caaatcagaa acaaattgat aaaggtgtaa
    10141 tgctaatgca tagagatagg actctagtcc ctagtagaaa aatgatagat tatgtaatgg
    10201 aaattgctga taaaaataat ataccacttc aacatgatat gtttgaatca ggtgggactg
    10261 atggaggaga agcacatctc gtaaatgaag ggaaaccttg tgtagtaact tgtgtaccag
    10321 ttagatatgg tcattgtgca ttttcaatag taagtaataa agatttagag aacattataa
    10381 gattgtatac acagttaatt ttaaattttg atgaagaaca atataaacat ataagaaatt
    10441 ttatctaaag gagaataaga aaatgtcaac agaagtacaa ataatggaac ttatatcaac
    10501 tgcaggagag agtaaatcga aagcttttga ggctttaaaa aaagtgaagt caaaagattt
    10561 taaaggagca aaattgttaa tagaggaggc tcgtaaaata gatattgaag ctcacaggat
    10621 acaaacagaa ttaataactc aagatttaaa tgaagatgtt gaaagtccag caatgagtct
    10681 tttaatggta catgctcaag accactatat gacttctcag ctagcaaaag acttgataga
    10741 ggctttgata tattgtcttg ataagtaatt gtaatataga aatagattat ttatgtaaaa
    10801 attcttttaa aagtttagat gcttttaaga gataataaaa atacaaactt taggaggaat
    10861 tattatgaaa atattattat gttgtgcagg tggattttct actaatatgt taatgcaaaa
    10921 tatgaaaaag gtggtaaaag agagtgctaa actcaatgaa aaagattttg agtttacagc
    10981 tataccagca gattcaatag aaagtgaagt tgatgattgg gatatagtac ttataggtcc
    11041 tcaaatagct cacaaaatag acttcttaaa aggaatatta gagccaaaaa ataagccttt
    11101 tgcagtaata gataaagatg tatatggaaa aatggatgga gctacagtta tgaagttggc
    11161 attaataact tataagaaac atcaattagc tcaaggataa taaaaataat gagggaaaag
    11221 ggagattaga tatgttttca aaatttgaag catttatgaa tagagtattt atgcctttag
    11281 ctcataaggt tgacaatcaa cgtcaccttg gttctataaa ggctggtatg gttgctatga
    11341 caccattcac aatattagga agtatatttg caatacttcc agcacttcca aatatgcttg
    11401 gagaaaataa tccaataagc aaattcatac ttaacaattc agcaatattg gatttaccag
    11461 taagtctttc cataggttta ataggattat atgcttgtat gtgtattgct tttaatctgg
    11521 ggaactacta taaattatat ataccaggtt gtgttgcact atcaactttt tcttttttat
    11581 tcctagtagc atcatttaca ccagatggag ctttagaaat aggaaattta ggagctaaag
    11641 ggctatttac aggtatgata gcagggcttg gttctgtgga gttatataat ttttgtaata
    11701 agaagaatat cacaataaaa atgccagaaa atgtacctga ttttgtatct aggtcttttg
    11761 aattaatacc tactactata atagtttgtg gaacttttat agttgctcgt tttatttcat
    11821 taaaagtatt tggagaatta cctccacaga tacttactca atttttatct ccattagtag
    11881 gaagtatgga taacccatgg tcagttcttg cacttaactt tgctatatgt actatattct
    11941 tttttggaat acattcatca gtatttagtc ctataactag acctataatg attacattta
    12001 tagctgaaaa catagcagct atgcaagctg gagaaacaat acctcatttc tttacagcag
    12061 gagcttcaag tgcattcttt ggatttacag gatgtggaat tagtataggt tgtgtaattg
    12121 catgtatgtt atcaaaaaat gcttcctata agaagattgg acgtgtatca ttattccctg
    12181 cattatttgg gataaatgaa ccaatattat ttggagcacc gataatatta aatccaataa
    12241 tgtttatacc acatgtattt ggtggagcta taataggtac actaccaatg ttttttatgc
    12301 actggggatg gttagcaaag ccagtatttg accctccata tgtaggggta ttcttagaag
    12361 gatttttaac taatggagat tggcattcta tattagcaaa tgcattgcaa ttagtattag
    12421 cagttttatt atattggcca ttctttaaaa taatggaacg tcaacaaaat aataaaaaac
    12481 aaaaagaaga aactaagaat atattctcta aagaagaaga agacttattg gatgatttag
    12541 gattggattt ctaatatttt gttatgaata atacaatgga gagattagta tccttaatct
    12601 ataaaagagg tgttgacgct cttttgatta aagcaaaaaa tactaaaaga tatataggag
    12661 ctttatcagg tagtggtgta tatgtgcttg ttactaagga taaaaattat caaattctag
    12721 atggcagata tattgatgaa gcagataaaa aaacgactgg tttcataaaa aggattgtat
    12781 ctcaaggttc ttatatacca actataattg agttattaaa agaattaaat ataaaaaagt
    12841 tagcaataga aaaccaagct atgagtatac aagagtatct ttcactaaaa aatgaaggtt
    12901 ttgaaatcaa tttaataaca aatgaattat ggaagctaag ggctataaaa tctaaagaag
    12961 aaattgaact tattaaaaag gcttgtgaaa ttacagatga agtatttgat gaggttattt
    13021 ctgaaattaa aaaagatatg actgagctag aagtatcagc tctaattcag tatcatgcat
    13081 taaaaaaagg tgcaagtgga atgtcttttg aaacaatagt ggtatcagga gaaagaggtg
    13141 caatgccaca tggaagacct acaaacaaaa aattgaaagt gaatgaagct ataacaatag
    13201 attttggtgt tgtttatcaa gggtatcaat cagacatgac tcgtacaata tctataggta
    13261 aacctcctaa aattattaaa gagatatatg atgtagtatt ggaagcacaa ttatctgcaa
    13321 tagagtcaat aaaggaagga actagggcaa gtgatgttga caaagtggct agaaaaatta
    13381 tagataaaca tggatttggc gaatatttta atcatggact gggtcatggt ataggtcttg
    13441 gagatggtga agtccccact ttaaatccaa atagtgaaga tatattagtg gaaggaatgg
    13501 taatgtcttg tgagccaggt atttatattc caaatgtagg gggagttaga atagaagatg
    13561 acattgttat aatagatgga aaaggtgttc cactcaataa gacatccaaa gagtttataa
    13621 tattaggaga gtaaaattat gaaatataat tttaataaag tattaaatcg taaagaagga
    13681 aattgtagaa aatggagcaa ccatgtgatt aaagaaaaat tcggcttaaa tgaagatgca
    13741 attcctatgg atttagctga tatcgatttt gagtgtgctc ctgctataaa agaatctatc
    13801 ttaaaaagag catcagtagg agattatagt tatactttta taggagatga cttctatgat
    13861 gcgattataa attggaataa aagaagattt gatgtatata tagaaaagga ttggataaaa
    13921 ttaacttttg gtactgtttc aacccttcat tatatagtcc aagcatatac taaagaagga
    13981 gagggtgtat tgataaatac acctgcttat gacccttttg cagaggctgt agtaaataat
    14041 aatagaaagt tgtgttgtag ctcattaaaa ttagataaca atagatatta tttagattta
    14101 gaggatatgg aaaatcaaat gaaatacgag aatataagat tgcttatatt ctgtagtcca
    14161 caaaatccat caggtcgtat atggacaaaa gaagaactat atcaagtgtc agagttatgt
    14221 ttaaagtata atgtaatttt ggtaagtgat gaaattcata gagatataat atttaaagat
    14281 tatagatttg tatctttatg gaatgctcat ccagaaattt ataaaaatag tataatctgt
    14341 gtatctccta ataaaggctt caacttaggt ggattaaaaa catcatatgt attaataaga
    14401 gatgtaaaaa ttagggagac tttattagaa agactaaaaa gtaattcaat aacttctcca
    14461 aatgtatttg caataccagc aatagttgca gcatataatg aaagcgaaga atggctagat
    14521 gctatgacta gttatgtaga ggagaatttt gaaatagttt atgatttctt tgaaactaat
    14581 ataccaaaag ctaaagtgat gaagtcagac tcttcttttt tagcctggat agatgtaaga
    14641 gaagtattta gaaatgaaga agaatctaaa gaatttttta gacatgcaaa cttaactatg
    14701 gtagttggaa gttattttgt gaaagatgga gatggattta ttcgtattaa tgtggggtgt
    14761 cctagagaaa cattaaatga ggctctaaat agaatcaaaa aaacgtatat aagtatgtat
    14821 tgttaaatca taaaaaaagt atctatggtt tatataaaaa cacattattt agttatcaaa
    14881 tattactgga attttgtatt ccagtaatat tttttgatta aggaatatta ttgaggaata
    14941 ttaaataata ttatttcata cccttatatt aacttatata aaaatatttt attattttgg
    15001 gaacgggtta gctaaccttt atctagtgta tatttatatt gtaagaagca gtaaaaactt
    15061 ttcctttact aagtttatta ataaacttct tatctttata aattttgtta gaatttatac
    15121 tatcagcaag accataccaa ggttctatac ataaaaaagg agcagtagaa ttagtttcac
    15181 tataataagg agtccaaata ccaactagag ggaaatcttc catagatata gttacatatt
    15241 tactaccatc attattacat atagatactt catctatgtt ggtgtatatc aaagcatcat
    15301 tcttgaatac ttctggattt aactgtaagt tttttaattt tccaataggt attatatcat
    15361 ctgtaaaaga accatttaaa ttgatttttt ctacatcatt tttactttta aactcaaggt
    15421 agtgtttaga aaaatcattt tgttcgtaaa aaggaaggtt gaatgcagga tgtccaccta
    15481 tagaaaagaa tatatcttta gaatcagtat tttttacaat ccattctacc tcaatactat
    15541 tatcatttaa tgtatagttt atgattagct caaaagaata tggatacttc tttaaagtag
    15601 attcattatc tgttaattta taagttacaa atgtatctcc tttatctaca atctcaaagt
    15661 ccatatctct agcaaaccca tgttgagtca tgctatataa attttcttct attattgttt
    15721 cattatcaaa aagtttacct actataggga acaatatagg tgattgtcta ccccaatatt
    15781 ttttattacc atcccaaaga atgtttttgt cgtatttttt agaaaatatt ttagttagtt
    15841 cagcaccaaa actgcttgat tcgataatta aattttcatt ttgtaatata ttcatttaaa
    15901 atctccttta ttatatttta tgagtatatt atactataaa gtataaagat tattaaattt
    15961 tatttgatat tatatttgaa aggtagattt tagtatattt tgaaaatttt gttttgaaaa
    16021 tttgggatta tttaagaaat tttgtattat aaatagaagg aaatagataa attgtataga
    16081 ataaaatata ttaagataag ttaataatgt taaatgggaa gtgagtttat ggaaaaacca
    16141 aatacaaaag ttgttaactt tcaagagtat aaagacagaa aaaaccaaag agaagtaaat
    16201 agagacgaac ttttaaagct tataaaaaaa attgtgactt caaaataggg agttgttaaa
    16261 ctccctattt taatgggttt cataataatt aagacattct gagtccatct atataatctt
    16321 tgaattgtga gaaatctgat tcactactta attctatggt ttctgaacct tccgtataaa
    16381 aattcttaag atagatagta taattattat caacacaatc atgataaata catctatatc
    16441 catcttcata caaacttttt aacttagaaa aatcatccac aatatcatct ccttaaagtt
    16501 cttttaaata tatttttaca aaaagcatct aattgtatgc aaattgacaa tgtaaaataa
    16561 aggaaatgaa cataaaatgt agaataattt ttatatgtat gtaaaaataa aaagaaaaag
    16621 ttgaaatttt tagaaaataa gagtattttt tatctaaaaa gagagtttaa aaaataaaaa
    16681 aatgttggga ggataaaaaa tgagtttatt agaaagtata tcaaaaaaca tatattccct
    16741 agataactct tctattgaaa aaacaaagca gagattggat aggcttatac atccaactgg
    16801 aagcttagga aagatagaag atatttgcat gcaattagca ggaatatttg gcaatgaaaa
    16861 ttttgacaca agtaaaaagg ttataattgc ttttgcggga gaccatggtg tatatgaaga
    16921 aggcgtagca cctgaccctc aaaatataac aaaattacag tttccaaatt tttcgaaagg
    16981 tctatgtgga gttggagtaa taagtaaatt tgtaggggca gatgttgtag ctgtagatgt
    17041 aggaataaac tgtgatgaaa aactggatgg ggtattagac tacaagataa gaaaagggac
    17101 atcaaatatg gcaaaaggtc cagctatgag taaacaagaa gcaattagat gtttagagat
    17161 aggtattgaa atagcagagc agtgtataga aaaagattat aaggttattg gtattgggga
    17221 gatgggtata gcaaatacta ctccaagtac agcaataata tctgtaattt cagggtgtga
    17281 cccgttagaa gttactggta taggtgcagg tcttaagaaa gaaagactaa agcataaggc
    17341 tgaagtaatt agaaaggcta ttgaaattaa taatccaaat ccaactgatg gagtagatat
    17401 tttatctaaa gtaggagggt ttgaaatagg ctcaatggca ggtgttatat taggttgtag
    17461 tgctaatagg attccagtag taattgatgg gtttatttct tatgcagcag cattaattgc
    17521 atataagata aatccaaaaa ctagagaata tatgatagca tctcatttat cagcagaatc
    17581 aggaactaaa agagcattag acatattaaa attagaccct ctattaaata tggatatgag
    17641 attaggtgag ggaagtggag ctgctttagc atttgatata atagaagctt caaactatac
    17701 ttataaaaat atggcaactt ttgatgaaat tgacatggga agataaatat aaaaatttaa
    17761 tatgtaggag tgttttgatg agtaaaatta ttctagtaac aggaggagct aggtcaggga
    17821 agagtaactt tgcagaaaaa ctatgttttg acagaaataa taatacagct tatatagcaa
    17881 cctctattcc atttgatgat gaaatgaaag atagagtaaa aaaacatcaa gagaatagac
    17941 ctagaaactg gaatacttat gaaatatatg aagatatata ttccataatt aaggagatat
    18001 ataaagaaca tgaaactgtt atactagatt gtgtaacatt attagtaaat aatctaatgt
    18061 ttgaacacaa tctaaatata gaagaatcta gtcaagaaga aataaacaaa ttagaacaat
    18121 acataaaaga acaagttgtc aaacttattg aagaaataga aaaaacaaat ctatactttg
    18181 tatttgtaac gaatgagtta ggtatgggaa ttgttcctgg gaataaactt agtagaattt
    18241 atgctgatat tgtaggtcgt ataaatcaat atatagcttc gaaaagtaat gaagtttatt
    18301 ttgtagttag tggcatagct atgaagataa aggagtagta tgcaattatg aaaagattta
    18361 ttttaatact acagtttttg acaaggatac ctataaaatt aaatgtagga tttgatgatg
    18421 aattttataa gtcaatagta tattttccac ttgtaggttt tgtcataggt atattatcct
    18481 atttaatagg atggatttct atgcttttat ttgaaccatt tatagcttct ataataataa
    18541 ctctagctgg tgtcttaatt actggaggac ttcatataga tggattagga gatacatttg
    18601 atgcaatata tagttatagg gataaagaaa agatgcttga aataatgaaa gattcaagat
    18661 taggtacaaa ttcacttcta gcaattatgt ttgtactttt attaaaggtt ggattcgttt
    18721 acgatataat aagtaataat tcattgtggg ttataatttt tatgccaatg attgcaaggt
    18781 taggagtaat gttattgaca tataaaactg tgacaccaag agaaaaagga atgggaaatt
    18841 tatttatagg aaaattaact acaagtatgt taataactgc tataatatac acattattga
    18901 tagttgctct tattactaaa tttatatttt tattacctaa tatagtgttg attaaagtat
    18961 taggttcgat tatagtggtt tttgtattta taatattatt caaaaaacat atttataaaa
    19021 aaattgacgg agtaacagga gatatattag gctgtggaat agaactttca gagcttgtat
    19081 atttaattta catatatttg ttaattttta tgttttttta aagtaaaaag tggtgataat
    19141 atgataagat taatattaat aagacatgca ctgacaaatg ataataaaaa aggaagatta
    19201 tcaggccata taaatagttg tattagtgaa gagggcaaac tacaaataaa taaaattacc
    19261 agatatctga gtaatgaaaa catagataag gtttatacaa caccatctac acgtactaaa
    19321 gatacagtag aaaaaatatc aaaattgaaa ttattagaaa tagaagaaaa agaagcttta
    19381 agagaaatta gttttggaga ctttgaagga agaacttttg aagaaattaa aatcaaatat
    19441 ccaaatgagt ttgaaaaaat gataaaagaa ggtaacaact atagatatcc aaatggagaa
    19501 agtttgatag actcttataa aagagtggca aaagaaattg ataatataat tttagaaaat
    19561 gatagtaatt tagatacaaa gacaatactt atatgttctc atgcaggaac aataagaaat
    19621 ataataacac atttgatttc tggtagttat aagtatcact ggaactttaa aattgataat
    19681 gcatctatta cagttttaga aattgatggt ggatttgctg taatagataa aatgaatttt
    19741 acagacttca tataatttaa tcaaaaccaa acattacaat ctgtgtttgt aatatatctg
    19801 taaaattagc aatgaaaata gaatattaga ttaaaattag gttcttaatt agatgttaaa
    19861 agggaaaaag gttagatgcc tttgcagccc ccgctactgt gaaaccaacg aaactataaa
    19921 taccactgtc agtttgatgg gaaggttata gaagtaggat gaagttaagc caggatacct
    19981 gcctaattta atttaaaaca agaggtcttc ggggtgaaag atttttaatt aatatatata
    20041 aaagtcctat ctttagaagt attaggactt tttttaattt gttttttagt aaattttaac
    20101 aaggagggat ggaaagtggg gaaaaaaata atgctacaag gaacagcttc aaatgtaggt
    20161 aaaagtgtct tagtagcagg tttgtgtaga atatttaagc aagatggtta tactgttgct
    20221 ccttttaagt cacaaaatat ggctttaaat tcttttataa ctaaagaagg attagaaatg
    20281 ggaagagcac aagtatttca agcagaggct gcgaaaatag agccaatagc tgatatgaat
    20341 ccagttttat taaaaccttc tggaaataat aaaagtcaag ttattgtaag aggtaaagtt
    20401 gtaggagaaa tgccttcttc taagtatcat gattataaat tggagttagt agatgtgttg
    20461 aaggaaacat ttaatagctt aaattcaagg tatgatattg tagttatgga aggggcagga
    20521 agtaatgcag agataaactt aaaagataga gatatctcta atatgggtat ggcagaaata
    20581 gctgatgcac ctgtaataat agtcggagat attgatagag gaggcgtatt tgcctcatta
    20641 gcaggaacaa tgcttttatt aaatgaagat gagaaaagaa gagtaaaagg tgtaataatc
    20701 aataaattca gaggaaaaaa agagctttta caagatggtg taaagatgct agaagacatc
    20761 ataaaagtac ctgttatagg agtagtacct tatacagata taaagataga agatgaagac
    20821 agtgtaacta cgagatttaa gaaaaaaatg aataaaagtg atatacacat agaaataatt
    20881 agaactccac atatgtcaaa ttttacagat tttaatatat ttgagacaca agaagatgta
    20941 agtattagat atgtagacta tggagaatct ttaggaaatc cagatatatt aataatacct
    21001 ggaactaaaa gtactataga cgatttaaca tatataagaa aaagtggtct tgaatcgcaa
    21061 atcaaaaatt tacatagtca aggtaaattg atatttggga tatgtggtgg ataccaaatg
    21121 ttaggtaaaa aattaaaaga cccttatcat gttgaaagtg aaattgaaga agttgatgga
    21181 ataggtcttt tagatacaga aacaacgttt gagaaagaaa agactacaac acaagtagaa
    21241 gctactattg ttgaatgtat tagtgaatac atgaataacc ttaagggaaa aaaagtaaaa
    21301 ggttatgaaa ttcatatggg aattacaaag cgaggtaatg atgtaagaag tttagattta
    21361 ataactaaaa aacttgataa agaagtgaat tatactgagg gaagtgtaaa taaagagggg
    21421 aatgtaattg ggacttatct tcatggaata tttgatgaaa taagctttac aagagcaatt
    21481 ttaaacaata taagaaagaa aaaaggttta ggagaattag aaagcaaagt aaactctttt
    21541 aatgaattta aggataaaga gtatgataag ttggctgatt ttttaagaga acatttagat
    21601 atagagaaaa tatatgagat tatgaaataa tgatgacctt tatttataat ttaggtagga
    21661 ggtgtattat gaaaaaaatt ttaattgcgg gtactaatag tggagtaggt aaaacaacaa
    21721 tatcattagg aataatgcaa gctttggtta agagaaatat gaaagtacag ccatataaag
    21781 tagggccaga ctatatagac ccttcatatc acacttttat aactggaaga cattctagaa
    21841 atttagattc ttatatgtta gatgatgaaa aaataaagta tatagtaaat aagtcctcaa
    21901 aagatgcaga tatttcagta atagaaggtg ttatgggctt atatgatggc tttggaatag
    21961 atttagataa ttgtacaagt tcacatactt caaaggtatt aaagtcacct gttatattag
    22021 ttataaatgg taaatctatg gcagcatcta gtgctgctat ggtacttgga tataaggaac
    22081 tagataaaga tgtaaatata aagggtgtta tagtaaataa tgtaaagact aatagccatt
    22141 atcaaataat taagagttct atagaaaaat attgtaatgt tgaagtattg gggtattttc
    22201 ctccaaataa caaattctct ttggaatctc gacatctagg attagttcct agtgtagaaa
    22261 tgaaatcact aagggaaaaa ttttatacct tagcagatga aattgaacac tatatagata
    22321 ttgacagaat aattgaaata tcagaaagcg atgaatttga gagtgatttt gattttcaaa
    22381 ctttaataga aaataataaa ataaaagaac atgttaataa taaatcaata gctatagcat
    22441 atgataaagc attcaatttt tattatagag aaaatataga gcttttagag gaacttggtt
    22501 tagaaataaa ctattttagc cctcttgaag atgtatcagt gccaaatgca gattatattt
    22561 atattggagg aggttttcca gaaatatttg gaaaagaatt agaagcaaat gagtcaatga
    22621 gaacttccat attagaagca cataacaata atatacctat ttatgcagaa tgtggaggac
    22681 tgatgtacct aggtgagaaa ctattgaatc aagaagaaca agaatttaat atggttggaa
    22741 tatttagtgg aattagtaag atgacatctt ctttaaaaag atttgggtac tgtattggtg
    22801 tagccaagga aaatactctt ttatctaaaa aaggggaatc tattaaaggg catgagtttc
    22861 atcattcaat atttgaaagt gatgaaaaat gtgtatattc aatgagaaaa gaaagagatg
    22921 gaaatatagt agaagagtgg gatggtggtt atagtaaagg aaacactttg gctacatatc
    22981 ttcatactca tttttataat aacttaaatt gtatagaaaa ttttttgaaa aaaggaaatg
    23041 aataaatgag tattttatct atttatatag gatatgtgac agacttaatt gtaggagacc
    23101 catattcttt tcctcatcca gtgagattta ttggtaaact tataaatttt actcaaggta
    23161 taataagaaa ggtctctaaa aatgataggc agttaaaatt ggggggattt atattatggt
    23221 ttataaccgt tggaataaca tatttaacaa cttatgctat agtgaagtta tttagtttta
    23281 atatattatt gagtattgta gtgaatagtt ttataattta tacaacacta gccacaaaat
    23341 gtttaaaaga cgaagcatta aaaatttata atgtattaaa aactgaagat atagaaaaat
    23401 ctaggattca gctatcttat atagtaggta gggatactac taatttaagt gaacctgaaa
    23461 taataagagc aactgtagaa actgttgcag aaaatactgt agatggtata attgcgccta
    23521 tgttttatgc atttattgga ggagcaccac ttgctatggc ctataaagct atcaatacat
    23581 tggactcaac agttggatat aaaaataaaa agtataaaga tattggtttt gcatctgcta
    23641 agattgatga tatagccaat tatataccag ctagaatatc tgtcattctt atgacaattg
    23701 gaagtttctt tttaaattat aactatagga actgttttaa aatctctatt agagatagaa
    23761 agaatcataa aagtccaaat tgtgcttttt cagaaggtgc agtatctggt gctctaggaa
    23821 tacaactagg aggtaccaac gtttattttg gggaaaaagt atataagcca acaatagggg
    23881 ataagcttag ggagatagat aaagaagata taattaaaac taataaaatt atgtatgcat
    23941 cttcatttgt atctatgttg gtatttacac taatatatgt gttatgtagg atgattttaa
    24001 ttaacttaat tatataaatt attgtttgtt aacttaaggg ggacatagat gaaggattta
    24061 gggcatggtg ccaatgtaga tgaaatggca agattatatg ggaaaaaccc aaaagaaata
    24121 attgatttta gttcaaacat aaatccaaat gtattaccaa atttagagag atacatttta
    24181 aaaggattag aagagtgtag gaattatcca gatataaatt atacaaactt gagagaaaat
    24241 atatcaaaat atatagatat aaatccagac tttataatac ctggcaatgg tgcaacagaa
    24301 gtgatttatt tgcttatgaa gagtattaaa aaaaaattgg ctataataaa tccgactttt
    24361 tcagagtata gaagaagtgc tgagttaaat aatttagata ttatagattt agagttagat
    24421 ttagaaaata actttaaact taatatagat ataataaaag aaaatataaa aagatttgat
    24481 agtttattta tatgtaatcc taataatccg agtggtaatg tccaagattt aaaggaactt
    24541 gttcatttac tagataaaca taataaggta ttaataattg atgaaacttt catggaattt
    24601 gttgaagatg aaagtaaata tagtttggtt aaatatatag aatcaaataa aaatatattt
    24661 ataattaaag ctgtgactaa gttttttggt atgcctggtt taaggctagg atatggtctt
    24721 acaagcaaca ctgaaattat gaacaaaatt tatgaacaca aagagccatg gactatcaat
    24781 tcttttgcag atatactatc caattttatt tttgaagata aagagtatat taaaaatagt
    24841 aaagaatatt acatagaaga aagagaatat atgttacaag agttgagaaa cataagaaat
    24901 ataaaggtct atgatacaga tgctaatttt atcttgataa aaatatataa aaaaacaact
    24961 aaagaattga aaaaagattt gtttaagcaa ggaaacatat tagttagaga tgcgtctaat
    25021 tttataggat tagatgatag ttttataaga gtggctataa aatctcatga agataacaaa
    25081 attcttatag aaaatatgaa aaatttattg ggtgattaaa atgaaatctt atggaatatg
    25141 cccagcatct tgtggagaat ttgtacaagg tataattgat gatgaagaat atttatgttc
    25201 atatgcaata gatatgtatt ctaaagtgta tattgaagaa aaattagtag atataaattt
    25261 agggagatat aagtcaaggt tagcaattga aaaggtattt gaaaaattta atttacctaa
    25321 aaaatatact aaaaatattt ctttaaatat aaatagtaaa atacctgttg gaaaaggaat
    25381 ggcaagttca actgctgata taggagctac tataaaggca acattatcat tgatagataa
    25441 agatttaagt agcgaggaga tttctaaatt agcagcagaa atagaaccaa cagattctat
    25501 atttatagat aaaaatagta tatttaatcc tctaaatgga actgttataa aatacctagg
    25561 aaatctgacc aatgcgaaag ttgtaatact tgaaccaaac aaagtactag atactatgaa
    25621 aattagattg aggcaagatt ataataaatt aaaagtagag aataaagaag taataaaaaa
    25681 atcatttgct cttttagaag aaggattgaa aaaaaataat ttatctctag ttggagaggc
    25741 ttgtactttg agtagcttag caaatgagaa tatagaaaaa aaagagtatt taaatgaaat
    25801 aataaaaata tcaaaaaaat atggagcata tggagtaaat attgctcaca gtggaactgt
    25861 agtaggtatt ttaattgata agtttatgaa tgataaaaag atgatagatg ccttatgtga
    25921 atctaacata aattctgtct ataataaaat ttatacacaa aacataatta atggaggaat
    25981 taagggggaa atagaatgga atacataaag aatcctatga aaatagaaga aaaaagtttt
    26041 gaattgatac aagaaataat agacgaaatt agaccagatt ataaatttaa gaataatatc
    26101 gaagaaaaaa taataaaaag agctatacat actactgctg attttgatta cttagatata
    26161 ttaaaaatat cagaagaagc agttgatagt ataatagatg cattaaaaaa taatgcaagt
    26221 atttatactg atacaaatat ggctcttagt ggtataaata aaagaaagtt agaatcatta
    26281 ggatgtaagt ataaatgttt agtggcagat gatgaaactg taaaattagc taaagaaaaa
    26341 ggaattactc gttctatggc agcagtagaa attgcagcta aagaagaagg aagaaaaata
    26401 ttcgttttag gtaatgctcc aacagcttta tataaagtta tggagatgaa atcagaaggt
    26461 agattagatt tagatgcagt tataggagtt cctgtaggtt ttgtaggagc acaagaatca
    26521 aaagatgaag ttgaaaaaac agatatacca tatataattt ctaaaggtag aaaaggtggc
    26581 agtaatttag cagcagcaat agtaaatgct atattatata ctatgtaggt acctatatgg
    26641 aagaatatgt atatatagat ggaaaaaaat atagaagagg atatactaca ggttcatgtg
    26701 caacaggtgc atctaaggct gctgtatata tgttgattac aaaaaataga attaatacta
    26761 taaatataga tactcctaag gggataccat tactactaaa agttgataat ataaatattt
    26821 ctgatacatt tgtggaatgt tccataaaaa aagatggtgg agatgatata gatgcaactc
    26881 ataccatgga tatttatgca agagctgaaa ttgtagctaa aaatgataag aacaaagggt
    26941 atcttactct aaaggatata gatagtttaa gcaccaatag tgagtgtaaa agtgagctct
    27001 ataagtttat tagggtttat ggcggaactg gtattggtgt ggttacgaaa aaagggctta
    27061 gtgtggatgt tggtaagcct gctataaatc cgactccact taaaatgata aatcatgaaa
    27121 taagaaagct tataggagat aattttgaat ctatacttgg aaatgataaa gttttaaaaa
    27181 taacaatctt tgctccacaa ggagaaactg tagcaaaaaa aacatttaat ccaagattag
    27241 gtatagttgg aggaatatct ataataggta caactgggat tgttgaaccc atgagtgatg
    27301 atggttggaa gaagtctcta tctatagaac ttcagatgaa aaaagaacaa ggcttagata
    27361 aaataatatt ggttccagga aatcatggag aacagtttat aagagaaaaa ttaaacttag
    27421 atattaagta tgtagttagg gtaagtaatt ttataggcta tatgattaaa gaagctcaaa
    27481 gaataggata taaaaaaatc ttaatggcag gccatatagg taaatttata aaagtatctg
    27541 ctggtatttt taatactcat agtaaggtag cagatgctag aagtgagata ttagtggcaa
    27601 acttagcttt aatgggagct agatacgaat ttttaaataa aataaatcaa tgcgtaacta
    27661 ctgaagaagc tgtagagcta ataaataata gtgaatatag agaagtatat aatatattaa
    27721 gtaataaatg tagggagaga gtaaagcaat acttgaatga agattcagat gatattgatg
    27781 tggaagtaat catattttca atggataagt ctttattagg taaatcggat aatacagatg
    27841 atttagtgga ggtctttata tgataaacat aataggtcta ggacctggta acttagacta
    27901 tataacaaaa aaaggtgaaa atttaatttc cacttctgat gtattaattg gtggtaaaag
    27961 aaatttagag tctataaaaa attttgaagg agaaaaaata gttctggatt ctaatttaag
    28021 agaaattata gagtacataa ataacaataa agaaaagcaa atatcaataa tagcatctgg
    28081 ggacccatta atatatggga taggtagata tctatctaaa aatattgata ataagatgtt
    28141 aaacatggtt tctgggatta gttcattaca gtacatattt tcaaaaatat atgtagatat
    28201 gaatgatgta tatattacaa gtagtcatgg caaagtacca gattttgatt acatattatc
    28261 acataaaaaa gtatgtatgg ttacagattc aaagatagga ccaaaacaaa taagtagaga
    28321 aataatagat agaaatctaa ataaaattat tgttgtagga gaaaatctgt catatgacaa
    28381 tgaaaaaata acaatagcaa aaccagaaga gataataaga atggacaatt ttgatatgaa
    28441 tgttgtggtg attttagatg aggaatagtg aatttataac aggtaaagtt ccaattacta
    28501 aggaagaggt tagagcaata tcaataagta agttaaattt agtaaatgct aaaaacctca
    28561 tagacatagg ggctggtaca ggaagtgtaa gtgttgaagc tgcatataat tatcctaatt
    28621 taaaggttat atctatagag aaaaatgatg atgctataga gcttatagag aaaaataaaa
    28681 aaaaattcaa cttaaaaaat atagaagtga taaaaggata tgctccaatt aatctagggt
    28741 taaatgttaa agttgagtct atttttcttg ggggaacagg aaataatctg gaagaaataa
    28801 tagaatggtc taaaaaaaat ctcgttacag gaggaagact tgtagctaat tttataataa
    28861 tagatacatt taatcaaact ttaaaactgc ttaaaaagca tggatttaaa gaaatagatg
    28921 tatgtgtttt gaatgtttct aaactagaga aactaggaaa aggtgaatat tttaaaccac
    28981 taaatcctat atatataata tcttgtgaaa aaggggaaaa tgatgatgaa taaagtacat
    29041 tttgtaggag caggacctgg agataaagag ctgataacat taaaaggata caaattatta
    29101 agtaatgcag atgtagtgat atatgcaggt tcacttgtta atcctgaact tttggaatac
    29161 tgtaaagaag attgccaaat acataatagt gcacatatgg atttacaaga gataatagat
    29221 gttatgagag aaggtataga aaataataaa tctgttgtaa gactgcaaac aggtgatttt
    29281 tctatatatg gttcaattag agaacaagtg gaagatttaa ataaattgaa tatagattat
    29341 gactgtactc caggagtaag ttctttttta ggggcagcat catctttagg ggttgaatat
    29401 acagttccag aaatttctca aagtgtaatt ataacaagaa tggaaggcag aactccggtt
    29461 ccagagaaag aatcaataca atcatatgct aagcatcaga catctatggt aatattttta
    29521 tctgttcaag aaattgagaa agttgtgtct aaattactgg aaggtggata tccaaaagat
    29581 actcctatag cagttatata caaagcaact tgggcagatg aaaaaatagt taaaggaact
    29641 ttaagtgata tagctgtaaa agttaaagaa aacaacataa ataagactgc tttaataatg
    29701 gttgggagat ttttaggaga agagtataat aattcaaagt tatatgataa ggatttcaaa
    29761 catgaatata gagggtaata ttgctataat ctgtattaca gaaaatggaa aaaaattagc
    29821 tctaaagata aattctctag taaataactg tgttatatat catgtaaaaa ataaaaaaag
    29881 taatttagtt gttgaagaaa catgtgtgca tacagttcaa aagaagttaa gtgattttgt
    29941 aggagaaata tttagcaaat ttgattacat agtatttata atggctacag gtatagttgt
    30001 acgaagtata gcaccataca taataagtaa gtttagtgac cctgcaattt tggtaatgga
    30061 tgaaaaaggc aaaaacgtaa taagtttatt gagtggtcat attggagggg caaacagctt
    30121 gacacagtac ataagtagtc ttattgatgc taatccagta ataacaacag ctactgatat
    30181 aaatcaaaaa gcttccttag atatgatagc aaaaaaacta aatgcacata ttgataattt
    30241 tagagaaagt gtaaaagaag taaattctat gctagtgaat aataaatgtg ttggattata
    30301 tattgatgaa ggatatcaag ttgatactag gggatttaaa cttatagaaa gtaaggatga
    30361 gttaagagat agtttttcaa acacatatat agaaaaatta gttgtagtaa gcaataaaat
    30421 aaattgtatt gaagatattg agaatttaaa cctagataat aaatttgaac attgctatag
    30481 taccaaaaaa ataataaagg taattccaag agatatagtg ataggaattg gttgtagaag
    30541 aaacacagat agtgaactga tgtataattc acttgaagag tttcttaata aacaaaatat
    30601 agatattaga gcaataaaca cagttggaag tatagaactg aaaaaagacg aaaaagcaat
    30661 aattgaatta tcaaaaatat tgaaggtgcc atttaaaaca ttttcagtag atgaaatatc
    30721 taaggttgat aatctttttg ataaatctga ttttgttaaa aaaaatgtgg gcgtgtattc
    30781 agtagcagaa cctgtagcat atcttttgag caaaggtaat ttgttagttg aaaaacataa
    30841 atataaagga ataacttttt cagtggggag aataaaaata tgatatacgt tgtaggaatt
    30901 ggaccaggaa gtaaagatac aatgacttta gaagcaataa aagcaataga agattctgag
    30961 gctattgttg gatataagac ttatataaaa ctaatagaag aatttataca agataaagaa
    31021 ataatccaaa atggtatgag gcaagaagtt gatagatgta aacaagcagt agaggaagca
    31081 aaaaaaggaa aaaaagtagc agttgtaagc agtggtgatg ctggaatata tgggatggca
    31141 ggtcttattt tagaattaat atctaaagaa gatgaagata taaaagtaaa ggtaattcca
    31201 ggtgttactg caagtattgg tgcagcagct atacttggag ctcctattat gcatgatttc
    31261 tgtcatataa gtttgagtga tttaatgact ccgtgggaag taattgaaaa aagattgaga
    31321 ttggccgctg aagccgattt tgtaatttgt ttgtataatc caagaagtaa gggaagaagt
    31381 gaacatctaa gcaaagcatt taaaataatg ggtgagttta aaggtggaga tacacctgtt
    31441 ggagtggtaa aagatgtagg tagagaaaaa gaagaaaagt ttgtatgtac ttttgaaaat
    31501 atggactttg aaaaagtcga tatgactaca atggttataa taggaaataa gtctacctat
    31561 attaatggtg aattgatgat aactccaaga ggttatacag tatgatttta attttaggag
    31621 gaacttcaga tagtttatct atatgtgata agataaatga actgaaaaat caaccatata
    31681 tcctttcagt tactacagat tatggaaggg aacttgcaca acaacatgca gagaatgtaa
    31741 ttttaggtaa gcttggaaaa gaagatatgc taaaatttat aaaagataat aatatagtaa
    31801 aaataataga tgccactcat ccttatgcag ttgaagtatc caaaactgct attacttgtg
    31861 caaaactatt gaatatagat tatataaggt ttgagagaaa atctttaatt gaagaaatag
    31921 attatgataa taaatttata gtagatacaa ttgaagaagc ctgtgaaata gcaaataaaa
    31981 tagggacaaa tatattcata ggaacaggaa gtaaaaattt gaacagattt atagaggaaa
    32041 taagtgataa aaatttagtt gttagagttc ttccaacgag tgaagtcata ttaagttgtg
    32101 aaaaattagg tttaagtgca gataatatag tagctatgaa aggtccattt agccagtata
    32161 taaatgaaga aacatataaa cattacaata tagatttagt cataacaaaa gaaagtggaa
    32221 tagctggtgg gttcttagag aaggtaaatg catgtaaaac tttcaatata ccagtaataa
    32281 ttataaaaag agaatcgatt aactacgaaa atgtatttaa tgacataaat aaaattaaag
    32341 acatacttgt gtaattttta ggaggagaat atatgaagaa agccttatta gtagtgagtt
    32401 ttggtacaag ctatcatgaa acgagagaaa agacaataga tatatgtgaa gataaaataa
    32461 aaaattcgtt aaaaagccat gattttttta gagcttatac atcaaatatg ataataaata
    32521 aaataaaaaa aagggatgga atagaaatag ataatccaat acaggcttta gataaaatat
    32581 atgaacaagg atatgatgaa gtaataatcc aaacacttca tattatatgt ggcgaagaat
    32641 ttaacaaatt aaaagaacaa gtagatggat attcttctaa attcaaaaag attgtattag
    32701 gaagaccttt gcttacacat attgaagatt atcaggaagc tgtagaggca attaagcatc
    32761 aaattcctca tatggaaagt aatgaagcag tagtatttat gggacatgga acacttcatg
    32821 aatcacattc agcgtatcca gcattggaat acatgcttag ggataatgga ataaatgctt
    32881 atgttggtac agttgaaggg tatccagaaa tagaacatgt aataagaaga ttaaaagaag
    32941 gaaatataaa aactgtaaat ttgatgccat ttatgttagt tgcaggagac catgctataa
    33001 atgatatggc aggagatgaa gaagattctt ggaaaacaat attagaagaa aatggattta
    33061 acgtaagagt tcatttaaaa ggactaggag aaaattctta tatacaagat aaatttgttc
    33121 gtcatgctgt aaaatgtgca gagaatgcga agttctatgg tataggagca gggccaggag
    33181 atggttcttt acttacgata aaagcggtta atactttaaa gaaactggat attctataca
    33241 ctccagaatc aaaaaagggt ggagatagtc tagctttatc aatagttagt gaatatttac
    33301 ctgaatcagt ggaaataaag tctaggcatt tcccaatgag ttttgatggg aatgaaaaaa
    33361 gtttagcttg ggacaatgtg gcacaagaaa ttatatcaga tgttaatgat ggtaaaaatg
    33421 taggatttgt tacattagga gaccctatga tttatagtac atatgtatat ataatgaaaa
    33481 gaatattagg agaggtagaa gtcgaaacta taccaggaat ttcatcattt tccaatatag
    33541 catctaatca gaattttcca ttagtcatgg ataaagaagc tttagtggta attccttgta
    33601 ctatggaaga tgataagata gagtatgctt tgcaaaatta taattcaata gtattgatga
    33661 aagtatataa aaattttaaa gaaataataa aaaaattaga aaagtatgat ttaatagaac
    33721 atgctatttt agtaagtaat tcttcccaag aaagcgaaaa tgtgtttaca gatttaaaag
    33781 aagcgcacct tgaagataag atatcttatt tttctactat actagtaaat aaagataata
    33841 aaataaaata ggtgaaagca atatttaaag gagacttaat atgaatatag tggttggaac
    33901 taggggtagt aatctagcgc ttatacaaac agaatgggta ataaatgaat taaaaaagaa
    33961 gtatccagaa attagttttg agattaaaat cataaagaca aaaggagatt taattcaaaa
    34021 tgtttcactg gataaaatag gtgataaagg attgtttgtc aaagaaatag agcaacaatt
    34081 attagatgga aaaatagaca tagcagtaca tagtatgaaa gatatgcctt cttatttagc
    34141 agatggacta aaatttgctc atacgcctaa gagagaagac ccaagagatg ttctaatact
    34201 tagagaagga tataaaaatt tagatgattt acctcatgga gcagtaatag gaacaggaag
    34261 taaaagaagg aagtttcaat tattgaaaca aagacctgat ttaaatatag tacaagttag
    34321 aggtaatgtg gaaactagga tacgaaaaat aaaagatgaa aacatgcatg gtattgtgtt
    34381 ggcagcatca ggaataatta gagctaattt gcaagataaa ataagttctt atttacctgt
    34441 tgatgttgtt attccagcac ctgcccaagg agctttagct atagaaatta gaagcaatga
    34501 tagtgcgata gagggaatag taaattcact gaaggatgag aatacagaaa tccaaatatt
    34561 ggctgaaaga ggttttttag atggtgtaaa tggaagttgt catattccaa tggcagctta
    34621 ttgtgaaata atccaagata aaatacatct tactggctta tatggagatt cagaaggaaa
    34681 aaaagtagta atcaagtcta tagatggaga tatttcttct cctagagagt taggtttaaa
    34741 gttagctaaa cttgtattga aggagtatga gaattatgaa gggtaaggtt tacttagtag
    34801 gggcaggacc aggtgattat aaactcatga ctttaaaagg tttagagtgt ataagaaaat
    34861 cggatgtaat agtatatgat agattagcaa atagtagtta tttaagagaa gctaagcctg
    34921 attgtgaact tatatatgta ggcaaggcat caagtaatca tattttgcca caagaagata
    34981 taaatagaat tatagcagaa aaagctaaag aaggaaaaat tgtaactcgt cttaaaggag
    35041 gagaccctta tgtatttggc agagggggag aagaagcaga gactttagta gatgaaggta
    35101 tagaatttga agtagttcca ggtataactt ctgctatagg aggtttatgt tatgcgggta
    35161 ttcctataac acatagagac catgcttcgt catttcatgt aataacaggt catttgaagg
    35221 gtgatgatag tggagaacta aactggaatg ctcttgcaaa taacaaaggt actcttgtgt
    35281 ttttgatggg aatttcaaac ctagagaaaa taagtgaaaa cttaatgaaa gaaggaaaag
    35341 ataaggatac acctgttgct cttataagtt gggcaactag atataatcaa agagttgtta
    35401 catctacact tgaaaatgtt tatgaaactg ctattaaaga agaagtaaaa ccgccaactc
    35461 ttatagtagt tgggagtgtt gtaggactta gagaaaagtt gaacttcttt gaaagtaagc
    35521 ctttatttgg aaaaagtatt gctgtaacta ggtctagaaa tcaaaatagt gtattggtag
    35581 aaaaaataat ggatttaggt ggaaatccta tagaaatacc tactattaag gtagaaaaaa
    35641 tacaaaataa tattaactta gaaaatgaaa taaaaaatat aaataagtat aattatctaa
    35701 tactaactag taaaaatgcc gtagaaatct tctttgaaaa gatatatgaa atgaattttg
    35761 atttaaggat attatctaat ttaaaggtat gtgctatagg ttctgctaca tcaaatgagt
    35821 tgaaaaaaag aggaattata gcggatatag taccaaaaaa atttgtagca gagtcactgt
    35881 atgaagagtt agcgcctatt ttaaatgatg acgataatat acttatacct agagcagaaa
    35941 atgctagaga ttatctggtt gacaaactta aggctatttg tgaagtaaca gaagtacata
    36001 tatatagaac agtaatagat gaggagaaaa aaggtgagat attagatatt ttaaattcta
    36061 atgacataga ttatataaca tttactagtt cctcaacagt aaataatctt gttgaaatta
    36121 taggaaaaga aaatattaaa atattagaaa aaataaaaac actatctata ggtcctataa
    36181 ctacagagac aataaatagt ttgggaataa aattgtacaa agaatctgct acatctacta
    36241 tagattcgtt agtagattta attataaaaa attaattttg gaggcttgaa tatgttaaga
    36301 agaccaagaa gactaagagc aagtaaagca atcagaaatt tggtaagaga aactaaggtg
    36361 aatgtagaag acttaatata tccactgttt gtagtagagg gggaaaatat aaaagaagaa
    36421 attaaatctt taccagatgt ctatcattta tcaatagata tgctagaaga cgaaataaaa
    36481 gaaataagtg agttgggaat tgaacatata atgttatttg gtgttccaga tgaaaaagat
    36541 gagtgtgctt cacaagcttt taatgataat ggaatagttc aaaaggcaat tagaaaggta
    36601 aaagaaataa attcagatat aaatgtaata acagatgtct gtatgtgcca atatacaagt
    36661 catggacatt gtgggatttt aactgatact ggttatgttg ataatgataa atcattggag
    36721 tatttggcta aaatatctgt aagccatgct aaagcaggag ctgatatggt tgctccatct
    36781 gatatgatgg atggaagaat acaagcaata agagaagctt tagatgaaaa tggattagaa
    36841 aatgttggaa tcatggctta tagtgttaag tatgcttcaa attattatgg accttttaga
    36901 gaggcagctc attcagcacc atcttttgga gatagaaaaa catatcaaat ggacccagca
    36961 aatagcaatg aagctataat agagactgaa cttgatatat tggaaggtgc agatatactt
    37021 atggttaaac ctgcattatc atatttagat attataagaa gagttaaaga taattttgac
    37081 ttacctctag cagcttataa tgtaagtgga gaatactcta tgcttaagtt tgctgtgaag
    37141 gaaggtttat taaatgaaga tgtaattctt gaatcaatta catctataaa aagagcagga
    37201 gcagatataa taataactta ttttgctaaa gacattgcta aactgttgaa aaaataaatt
    37261 ctagcagtat aaagaatatc aaaaagtata ggaattgtaa ttaactatta tacagtagtt
    37321 ttgtttagac atttattgaa gaataataga aactaatttg caatttctat actttattta
    37381 tgtaactaaa taaaatttgg aggtagaaga tgctatatcc tattaattta aaattagatg
    37441 agttagatgt agttataatt ggtggtggag aagttgcata taggaaatgt aaaaattttt
    37501 tagagtttaa caaaaatgtt actatagttt ctaaacaaat tttaaataaa ttttatgatt
    37561 taaaaggaaa tataaaaata ataaaagatg attataaaga aatatatata aaagatgctt
    37621 ctgtaataat tgctgccaca aataatagag agttaaatat ggaaatagga ttatattgta
    37681 aagcaaaaaa taagctagtg aatgtagttg ataatataga aatatcgaac tttactgttc
    37741 catcatatat aaaaagagga gatttactta taagtgtgtc tactgggggc aagagtcctt
    37801 cattatcatc aaagataaaa aaagaaattg aagagagata tactgaagat tatgaggagt
    37861 atttaaatgt attaggagat ataagaaaaa aggttataaa aaaatacgaa gataaaagta
    37921 aaagaaaaga tgtgcttaat atgttggtaa cacttgacct agaagaactt aagaagtttg
    37981 atatataaaa aataaaataa aaaatgttaa aaaggtgttg acggattatt tttaagatga
    38041 tatagtatta cttgtcctta agaaaagggc aaacataaaa aa
//
LOCUS       DACULQ010000017        35843 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.017, whole genome shotgun sequence.
ACCESSION   DACULQ010000017 DACULQ010000000
VERSION     DACULQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.017"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(128..898)
                     /locus_tag="JA154_03695"
     CDS             complement(128..898)
                     /locus_tag="JA154_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellobiose-phosphate degrading protein"
                     /protein_id="HAT7994962.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(900..2327)
                     /locus_tag="JA154_03700"
     CDS             complement(900..2327)
                     /locus_tag="JA154_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS acetylglucosamine transporter subunit IIB"
                     /protein_id="HAT7994963.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGVRLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            2718..3440
                     /locus_tag="JA154_03705"
     CDS             2718..3440
                     /locus_tag="JA154_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7994964.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            3637..5304
                     /locus_tag="JA154_03710"
     CDS             3637..5304
                     /locus_tag="JA154_03710"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAT7994965.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYYLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(5400..6719)
                     /locus_tag="JA154_03715"
     CDS             complement(5400..6719)
                     /locus_tag="JA154_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT7994966.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRQTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(6760..7497)
                     /locus_tag="JA154_03720"
     CDS             complement(6760..7497)
                     /locus_tag="JA154_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAT7994967.1"
                     /translation="MAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVMCKA
                     IPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSFDMK
                     LLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTHTVQ
                     IKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFVIGI
                     QWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(7750..9759)
                     /locus_tag="JA154_03725"
     CDS             complement(7750..9759)
                     /locus_tag="JA154_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT7994968.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(9813..9944)
                     /locus_tag="JA154_03730"
     CDS             complement(9813..9944)
                     /locus_tag="JA154_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994969.1"
                     /translation="MAQFLLYRSLRNMIDIATMIFMDIKLIKEYVSILNVQFFVLLK"
     gene            complement(10153..11586)
                     /locus_tag="JA154_03735"
     CDS             complement(10153..11586)
                     /locus_tag="JA154_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAT7994970.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(11673..13589)
                     /locus_tag="JA154_03740"
     CDS             complement(11673..13589)
                     /locus_tag="JA154_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit EIIBCA"
                     /protein_id="HAT7994971.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGVFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(13663..14523)
                     /locus_tag="JA154_03745"
     CDS             complement(13663..14523)
                     /locus_tag="JA154_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator LicT"
                     /protein_id="HAT7994972.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(14874..16061)
                     /locus_tag="JA154_03750"
     CDS             complement(14874..16061)
                     /locus_tag="JA154_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HAT7994973.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(16075..17481)
                     /locus_tag="JA154_03755"
     CDS             complement(16075..17481)
                     /locus_tag="JA154_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAT7994974.1"
                     /translation="MGRLKMSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFER
                     VDIDGRSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKT
                     GAIEAVIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGY
                     DSLTAVATVFIGATAGFGAATTNPFSVGIAQALSQIAPGSGIEFRVVMFIIYMAISIG
                     FVMMYANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIY
                     GVLKLGWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGI
                     VIIAENGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVL
                     APLGDLVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMV
                     VLTILVMAFLTIGLYLGF"
     gene            complement(18061..19713)
                     /locus_tag="JA154_03760"
                     /pseudo
     CDS             complement(18061..19713)
                     /locus_tag="JA154_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
     gene            complement(19733..21136)
                     /locus_tag="JA154_03765"
     CDS             complement(19733..21136)
                     /locus_tag="JA154_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HAT7994975.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(21400..21891)
                     /locus_tag="JA154_03770"
     CDS             complement(21400..21891)
                     /locus_tag="JA154_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAT7994976.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(21917..22729)
                     /locus_tag="JA154_03775"
     CDS             complement(21917..22729)
                     /locus_tag="JA154_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAT7994977.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLSKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(22742..23893)
                     /locus_tag="JA154_03780"
     CDS             complement(22742..23893)
                     /locus_tag="JA154_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAT7994978.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            24163..24804
                     /locus_tag="JA154_03785"
     CDS             24163..24804
                     /locus_tag="JA154_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAT7994979.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            24830..26374
                     /locus_tag="JA154_03790"
     CDS             24830..26374
                     /locus_tag="JA154_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT7994980.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(26574..27200)
                     /locus_tag="JA154_03795"
     CDS             complement(26574..27200)
                     /locus_tag="JA154_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994981.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(27335..28051)
                     /locus_tag="JA154_03800"
     CDS             complement(27335..28051)
                     /locus_tag="JA154_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HAT7994982.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDKAATKFGLE"
     gene            28477..29691
                     /locus_tag="JA154_03805"
     CDS             28477..29691
                     /locus_tag="JA154_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAT7994983.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            30311..31639
                     /locus_tag="JA154_03810"
     CDS             30311..31639
                     /locus_tag="JA154_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994984.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(31767..32957)
                     /locus_tag="JA154_03815"
     CDS             complement(31767..32957)
                     /locus_tag="JA154_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7994985.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSKNGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGSVPNAIPNKASMSGTLRTFDQSVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(33063..34442)
                     /locus_tag="JA154_03820"
     CDS             complement(33063..34442)
                     /locus_tag="JA154_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7994986.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(34473..35681)
                     /locus_tag="JA154_03825"
     CDS             complement(34473..35681)
                     /locus_tag="JA154_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994987.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIIPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT        11974 a         6282 c         4121 g        13466 t
ORIGIN      
        1 tgtacttcct atattgatag agatgattca agtttttatt ttgatacatc tctatcaata
       61 tttttttaga aaatacacat ttttttcaaa tacttatttt taattaaaac tttaggtaat
      121 ctattcttta tatataatta ttagacttga aaatatcttt aaaatcaacc aattcaatat
      181 tatttctttt taaccacatt ttaacttcct tatcacatag cattgcatgt tcatatactc
      241 tctcttgaat aagagaagat gattttataa gactataatc tataaatcct ggatgagtaa
      301 ccatcaatgt tatatcttta tctaaattta tatatttttc ttcaaataat ttaacaaaag
      361 atttatttaa cttgtaaaaa tcattctgag gatatttact tggcatgcta atacatgaat
      421 ccaaataata gggacaactt ggtataccgt attcttttgc agcatcacac attgctgata
      481 ttgatacatc ttcttctata ccatgtatat caatatactc aggcaattta ctcatataca
      541 acttgtaagt ttcaatttga gcacaaactt cttcatatac ttcttcataa ttaaaaacct
      601 ctttacctgt cagtagttgc gttcttctta ttttgctaga aacaaatttt ccaccttcat
      661 ctaacaaatt ctttatctta atatgttctt tgcaacaagg ttctcctatt actaaattaa
      721 catgtaaacc aaaacataca tcatcatttt ttaccatttc tatagcttgt cttgaaaaag
      781 gcatattcac catgattcca gcacttgtaa caataccgtt cttataagaa gatattatcc
      841 ctagtgtaac accttctgaa tatcctatat catctgccct aactactagt ttcttcattt
      901 tatgcgtctg cctgctttct caactcatca agttgcattt ccatctcatc ctttacttta
      961 acaacatccg ttcctatgat gatttgaact gatgtattac tcaatttaat tacccctttg
     1021 gctcctgttt ttttaatatt atcatcattc aaaatagtag catccttcac ttctaaacgt
     1081 aatctcgtca tacaattatt aagagtcact atattttcat aaccaccaca atttttaatt
     1141 atctgtttag ccatatatga aaaattacca tgagaaagtt ttaaattctt ttctttttca
     1201 ctaactttat cacctatctc tatctcaact tcacgtcctg gtgtttgcac attatctttt
     1261 attattatat atttaaatgt tacataatat aatacaaaga atacaactcc tacaggtata
     1321 actaaccacg ctttatctcc ataactaaaa cttaagacat agtcaattat acttgctcca
     1381 ttaaatgaac ccaagcgaac acccagtaaa tatagtacaa ctccacctag accagaataa
     1441 aaagaatgaa ttatatataa ctttggagat gcaaataaaa acgaaaattc tattggttca
     1501 gttactgttg ccataaaaga agtcaatgct ccagcggtca ttgcaccttt tacttcttgc
     1561 ttctttttat cttgtgctgt tttatatata gcataagaaa tagcaggtat tgaaaacatc
     1621 ataattatgt aaaatcctgt ccagaagaat cctgcatttg ggtctcctgc cataaatcta
     1681 ggaatttctc ctcttataac ttctcctgtt gatgttgtat attcacccat ttcaaataat
     1741 atgtaagcat ttaaaacttg atgtaaccca gttggtatta gtgcacggtt acagaataaa
     1801 aacaagaata ttcctattgc accagctcct cccaaccaaa aaccaaattg attcattgca
     1861 gcttgtggat atatccataa taaactaaat atatatgata aaattaaagc aaccactggt
     1921 gatagtgtaa ttacacattt ttctcctgca aagaagctta aaaatgctgg aactttaact
     1981 ttatatgttt tattgtaagt ccatgctata caagctccaa caattatccc accaaataca
     2041 cctgcattta atgtatcatt caataactct aaatttgact taaatatcat atatgcaact
     2101 acggctgcaa ccattacact tgtcttgtct ttagactttg agtaggcact agctgctcct
     2161 aataaaaata agtagtccac atttgaaaat actatattag atatattaga tatagctggc
     2221 acattgagca tatttggacc aaataataat cctaatatac ctgctgctgg cattacagct
     2281 attggcacta gcatagtctt tcctaaaagt gacagttgtt gcatcatttt gtttaagaac
     2341 ctcttcatat ccaatcccct ttctatttcg gtttaatttt ttgtgaaaac gatttattca
     2401 cattttatat tttaaatatt atatttgttt aaacaaattg tcaatatttt ttattcattt
     2461 tatttttatc atttagttta acatataaat ccttttaagt gtattgctat atctagtcta
     2521 tttttttaat tcattttttt tattttaaaa atatgtttaa ccatgtattt gaaacaaccc
     2581 acaaaataat tctattttgt cctttttatg gttgatatga ttctattttc actatacatg
     2641 tctaaacaca tttgatataa tttgttcata tcagttcaat aatattacta aagaccttta
     2701 aggagggata ggggtatatg gctaaaaata aattttcaga gatatatgaa gtattgaaag
     2761 aggaaatact tgatggtaaa tatacaagta atatgatgct gcctactgaa cttcaactta
     2821 ttgaaaggtt ttcatgtagc agaaacactg tacgtagagc aatttctcaa ttaaacacag
     2881 aaggatatgt tcaaagtata aaaggcaagg gtgttgtagt gttagaaaat agttgttcta
     2941 atgatttttt tctaaatatg cacaatttca aaggtgtaga atcgattgta gaagataaaa
     3001 aggttaatac tgctactagt gttctccact tcagcaaaat cttaattgat aataagttat
     3061 ctaaaaagac aggttttaaa gttggtagtg aagtatatta ccttcacaga ctaagatata
     3121 ttgataacat acctaaaatt ctcgacatta actattttct atgtagtata gttaaagacc
     3181 ttgatgtttc tatagcacaa ggctctattt ataaatatat agaagagtgt ctaggtacaa
     3241 aaattgtttc atccagaaag atatttaaaa ttgaaaaagc tacagagctt gagttaaaaa
     3301 cgctacctct aaatgattat aattgtgttg gtgttattaa aaatagtgta tatactgatg
     3361 atggaaagct ttttgaatat actgaatcaa agcatacacc tgaaacattt gtattcatgg
     3421 atgttactca aagatattaa aaaaattatg tttaattaaa cataattttt ttacgtttag
     3481 acactttatc ccatactaac ctcactattc tatatacaat tatgtgttta tacaaataat
     3541 tatttattgt atattttagc tttaatatta gtttaatgct tgacaatttg tttaaacata
     3601 ttataattaa cttataaatt acaggaggta tcaattatga aaaattggtg gaaaaaagca
     3661 actgtctatc aaatatatcc aaaaagtttc aaagatagca acaatgatgg tataggtgat
     3721 ataaatggta ttatagaaaa attagattat ctgtattatt taggtgttga tttattatgg
     3781 ttgactccta tgtacgtttc tcctcaaaga gataatggat atgatataga agattattat
     3841 aatatagacc caaaatatgg gacaatgagt gactttgaaa aactcttaaa agaagctcat
     3901 aaaagagaca taaaaataat gatggatatg gtcctaaacc atacatctac tgaacataaa
     3961 tggtttaaag aatctaaaaa aagcaaagac aatccatatc gtgattatta tttctggaaa
     4021 gatgctaaac ctgatggttc tgttcctaat aactggatat ccagatttag tggaactgct
     4081 tggaaatatg atgaaaccac taaccaatat tatttacatt tatttgaaga aactcaagct
     4141 gatttaaatt gggaaaatga aaaggttcgt gaagaatgtt acaaaatact agaattttgg
     4201 gctgataaag gtattgatgg ttttcgttta gatgtagtca acctactctc caaaacacct
     4261 ggattacctg atgaccctat aacaggacca aaaggagatg gaagaactca ctacgctgat
     4321 ggtcctcgta tacacgaata tttacataat atgaatcaaa aagttttcaa acctaaaaat
     4381 atagttactg taggtgaaat gtcatctact acacctgaag agtgtatcaa ctatactaga
     4441 gaaaatcgag aagaactatc tatggttttt agcttccatc atatgaaaac agattataaa
     4501 ggaggcgcaa aatggactaa tgaaatgttc tctctggata aattaaaaaa agcacaatct
     4561 gactttcaat ataaaatgta tgaaggaaaa ggatggaatg ctctatttta ctctaatcac
     4621 gaccaaccta gagctttatc tagatttgga gatgatagat tttttcgtca agaaagtgct
     4681 aagatgttag ccataacact atttggtcta caaggtacta catacatata tcaaggtgaa
     4741 gaaataggta tgacaaatgc ctactttact aaaatagatg aatatgatga tgctgaaagt
     4801 aagaatgcat actatcatat gattaaatct ggtattgatg aaaaggaggc attgcttata
     4861 cttcaacaaa aatccagaga taatgcccgt actcctatgc aatgggataa tactatttat
     4921 aaggggtttt ctaatcataa accgtggtta aaagttaata atgacaatat tagtgtagaa
     4981 aatgagctta atgattctcg ttcagtattt tatacttatc aaagattaat caaatatcgt
     5041 aaacaatatg atatttttac agaaggtact tatagattgt tagatgaaaa tcataaaaat
     5101 ctatttgttt atgaaagaga gtataaaaac caaaatcttc ttgttataag taatttcact
     5161 catgataatg tagtatataa attacctgaa acttatttga ataaattaaa ctacacaata
     5221 cttgaaacca attacactag agatataatt gaaaataaaa tggaaataaa accttacgaa
     5281 agtataatga tatattataa ataaagttat ttatgtaaaa ataggtataa caccctggtg
     5341 ttataccaat tttactaaat tcaaagtaat gtttatagaa tatctataga ttacaataat
     5401 tatttgactt ctacatcatt atatttcttt gaatatactt tagtcatata gaaatatata
     5461 ggaactacaa ttatcatccc acctaatccc caaactatct tcataggttc atcaatacct
     5521 gcttttatca gtaataatat acttataata actgcaaata ttggtataat tgctcctcca
     5581 ggtattctaa aatttacatc tttaccagca tacttctttc tcaatattag tacagctaaa
     5641 catgtaggaa tatattgaat aaatctagat acaacactta tttgagataa tgttgtgaat
     5701 gaacccgacc atgctattag taaagttcct atgacagata ctataattgc tatataagga
     5761 gctccatttt tattagtttg tcttatagac ttaggcatta atccttgttc aaccaaagca
     5821 gcagcagacc ttggtgtaaa tatggatgat gcaatattta tacctccaac agatactata
     5881 gttcctattg taattatata cccacctata ggacctaaca tcttaatggc agcatctgca
     5941 acaggagtag ctgaccctgc taagccatta cccaacaccc ctatacttat tcccagtata
     6001 agcatatata ttactgatac tacaaacatt accattacca atgctttagg tacatttttc
     6061 ttagggttct ccatatcttt tgctgcaaca gctaaagatt caaatcctgt aaaagcataa
     6121 aataaagtta tagatactgc tacaaaagct gatgacgcac tttgaccttt ttgtattatt
     6181 acaaatgggg taaaattaga accctttata aagaataatc ctacagctat aaataaaact
     6241 attggtaata attttcctat agtcatgata ttatttaagt gcttagattg tttcattccc
     6301 ataaaattta taatcccaag ccctacaact aatataccaa ttattatatt tttaataagt
     6361 ggttctgctg cttgtggcca tacagatgat agtgttacag caaagaaatt tgccatagta
     6421 gcccaagcaa tcatactaac tatccacttc ataaatccaa cttcaaatcc aacaaaatct
     6481 ccaaatgcct cttttgcata aagatagggc cctccatcag tatcaaacca actacctgct
     6541 tcagcaaaac ataaagcaat agaaacagct aaaagcatac aaaaaactaa tactaatata
     6601 cttgctggac ctatatctgt ataagcatca ccaggtaacc cgaatatacc tgaccctatt
     6661 attgcattga ttcctaatag tacaatactc caaaaaccta acttaatgcc tttttccatt
     6721 gttattcccc ccaaagccaa cttaatatat ttgtattttt tatttagatg cttctttaac
     6781 tattgccata aatatttttt tcatattatc ataatctcta gtcatcattt ctggatgcca
     6841 ttgtatccct attacaaact cacttccttc cttttctata gcttcaatta aaccgtcttt
     6901 agatgtagct gaaacaatat atcccggtgc tactttgttg acaactaagt gatgaaaact
     6961 attaactaaa acttcttttt ctcctagtat ttcatataat tttgtcccct cctttatctg
     7021 tactgtatgt gtaggtacat tagatagatg ttgctgattg tgtttaatat aagcaccttc
     7081 aattaaagat aaatcttgat acaaactacc tccttcagtt acatttataa tttgttctcc
     7141 tctacatata cctaaaactg gttttttcat atctaaagca tgttttaata atttcatatc
     7201 aaaactatct ctctttggtg atattgcccc caacttatta tgaggttctt ctttccatat
     7261 gagtggattt acatcttgtc ccccagatag tataagcgca tctatattag atacctgctc
     7321 ctttataatc tcatcatcat atacaatcgg tacaatatat ggtatagctt tacacataac
     7381 tactgattgt atataatcat tatttacata agccctttca tagccaggaa acattcctcc
     7441 ttcatcaatt agtaaattac ctgatattcc tattattgga tatttctttt tagccaattt
     7501 cttcacccct taacttattt gtgaacaata aaaaatgcta cttctcttaa agagcaagta
     7561 gcataccaag attttattta tttgaacatt ataatttatt ttagaaatac tttttttgaa
     7621 tacgagtaat ataaataact ttatttactt atattttatt ttaatcctgt ttagtttgac
     7681 ttttttaaat tgttttaagt acaactaata ggtaaaatag gcatttagat tttccttttt
     7741 atcaacctat taaatactgt ttaatatatt ttttcccctt ctatttaatt cacttccctt
     7801 tctaccagat tcacatatta tcaaatccag ctcttttaac atattaagta ttcctcttac
     7861 ctcaccttct gttatattgt agcctaattc atttattgaa ttaacaatac catttctacc
     7921 tatattttta ttaagattat tggcattttt taaacactcc attactaact ttactttctc
     7981 tatagatgtt ctatccttta atatgcttat ttctttacta aaatcttcag ttatatttaa
     8041 taagttatga ggtaaatcat ttattaaaat aacatcctca gaactcataa tgttaatata
     8101 catagcagta ttttttaatt ctcttatatt acctggccat tcatatgcat taataatgtc
     8161 ttgaacatct tgactaattg ctctcttcct tttcataaaa tgctttaaca taacttctat
     8221 atcttctttt cgctctctta aaggtggtat atttatgggc aatacattta tcctatagta
     8281 taaatctgct ctaaatctat ctccttttat catttctaat aaatttttat ttgtagccgc
     8341 aattactcta acatctatat ctattacatt ttctcctcca accctcatta cttgattctc
     8401 ttgtaatact ctaagtaatt tgctttgtaa gtatataggc atgtctccta tttcatccaa
     8461 aaatatagtt ccattgttag cctgttcaaa taatcctttt ttacctcctt ttaaagctcc
     8521 tgtaaaagca ccttcttcat atccaaaaag ttcactttca agtaaatttt ctggcatagc
     8581 tgcacagttt attgctataa atggctgact atgtcttggt gatgcattat gaatagactg
     8641 agccatgagt tcttttcctg ttccactttc tcccgttata agaattgtat aatcagattc
     8701 agctattttc atacctaatt tcttagttct ttccatcttc gttgaaactg tttttatatc
     8761 atcaaaagta tatctagcaa cttgtccctt ttctctaagc ttctttgata ggttttgctc
     8821 caattttctt atgtaagtta tttcttgaat acaaaaataa tatccagtaa cccttccaaa
     8881 actctctata ttatacttat ttacgtttaa gtacttatta ttaaatttta taacttcatc
     8941 atgtgcctta ctcaaactaa aaatcttttt aatattctca ttctctaatt catctatatt
     9001 tttccctata atattacttt gaatatctag tatatccctt aaactattat tacaaataat
     9061 tatgtttccc ttatctgata ccattgccat acctaagttt gataaattta ttatggaatt
     9121 tagttcatct gcttttatag taagttcttt gtatttagta tttattcctg aatataaact
     9181 aattatttca tctgtatact taattagcct ctcagtaatt tctttatcat ccagtttgag
     9241 ttttgctatt atttgaataa acgttgaact atcaatatac ctatggccta aatcaataac
     9301 ttcttctata tattttggaa ctaaacttga ctctcctggc gttatagcta tcttaatatt
     9361 tctatattct tcattgggat tgtatggtat aaaattcaaa tgatttattc ctatattgta
     9421 taaattagat attgtttcca atacagtatg cttattatcg tttactacta atacatctat
     9481 tccttctggc aatgaaaata acttatatat atccttttgc tttatgctcc tatttatagt
     9541 tattatcttt gagacatctt ccacatattt ttttattttt aatactctat catctatcat
     9601 taccaatatt atgtcatctt ttattaattc cttctctgtt attttattta aataataatt
     9661 ttttatcatt actttatctt taaagatcat ttctagattt ttatttaaga aacattctaa
     9721 tttagacaac ccatcagtta ctactgctat tgttttcatt tgattactcc ttttaaaata
     9781 tactctactc ttttataata tacatttgat tttcacttta ataacacaaa aaattgtaca
     9841 tttagtatac taacatattc ctttattagt tttatgtcca taaatatcat agttgcaata
     9901 tctatcatat ttcttaaact tctataaagc aaaaattgtg ccaaattaat aattttctta
     9961 attttattct aaagttcctc aatagtattt acagacttag cacttatagc ttataagtct
    10021 ttatattatt aatctatttt tataggttaa ttgtacaaat catatatcac ttttacctat
    10081 taaatgcatt ataaaaaatt gtatttataa acactattta ctactcttct aagtacaaac
    10141 agtatctaca tactataacc tttcaccatt agattctatt acttgtttat accagtcaaa
    10201 actctttttc ttaatcctag acaagtctcc acttccatca tcatacttat ccacgtatat
    10261 aaacccatat ctcttggcca tctcaccagt agatgcactt actaaatcta tacatcccca
    10321 tggagtatat ccaaataaat caacgccatc ttctacagct tccttcattt gctctatatg
    10381 tgcttttaaa tactctatac gatagtcatc atttatcact ccattagatt cttttttatc
    10441 ataagctcct aaaccatttt caactaccat cataggtatt ctatatctat cataaatttc
    10501 atttagtgca tatctaagcc cttcagggtc tatttgccat ccccattcac tagacttaag
    10561 atatggattt ttcactccac ctaatatgtt gccacttgta ttatcttctt tagaatcact
    10621 acttacacat gtagacatat aataactaaa tgtataaaaa tctacacaac cttcttttaa
    10681 aatttctaaa tctccttttt ccatttttat ttcaatatta ttttctccaa aatatctatt
    10741 catataagtt ggatattcgc cacgcacttg tacatctcca cagaaataat tcattactct
    10801 actatctctt tgagttttaa ctgcatcatc tggattacaa gtataaggat acattgttgc
    10861 aaatatttgc atacatccaa cttgataatt tgaatctatt tcatgagcta acttaacagc
    10921 cttagcactt gctacaaatt gatgatgtaa cccttgaaaa cgaagttgag gtatatctac
    10981 ttggtttata aaatctgtag tcccttcatt taatattcct tgtgataagt agcctcccat
    11041 aggcattgta gctgaattta tttcattaaa tgtcagccaa tattttactt ttcctttata
    11101 tcttttaaat attgtttcac agtaattcat atacaagtct attacatctc ttgaaaccca
    11161 tccattgtat ttctctgtta ttgcaaatgg tatttcatag tgagatattg ttattaatgg
    11221 ttctatatta tattttaaac attcatctat cacattttca taaaatttaa gtcctgcttc
    11281 atttggttct ttctccattc cagttggaaa tattctagtc catgctattg aaaatctaaa
    11341 taccttaaat cccatttctg caaataaagc tatatcttct ttgtatctgt gataaaaatc
    11401 tattgcttca tgacttggat aaaatgttcc tttttctata actctcgtta ttcttttact
    11461 tactttttga gaacctccag tacacatatc tgaacaagaa ggacctttcc catctaaatt
    11521 ccatgctcct tcacattgat ttgctgctgt tgctccaccc cataaaaaat cttttctaag
    11581 tgccataatt ctacctccaa taatttattt atagtaactt tattttaaaa taaaaataca
    11641 tatatataat tcttaattaa tagaactctc ttttacgcta aaacagttat caatttctga
    11701 ctattattta catgcttttg tgtattttct acaatatcca cataatcaga ataattagta
    11761 acaactactg gtgtttctaa gcaatatcct tcttttgaaa tactttctat gtcaaaagtt
    11821 attaatgtct gacctttttt aatcttatcc ccttgattaa caaaagattt gaaatattta
    11881 ccttccaact gaatagtatt taaccctata tgtatcaata gttcacaacc attatcagat
    11941 ataattccta ttgcatgttt tgttggaaat aaagtcatta cagtcccatc aaatggtgcc
    12001 actacttttc cttctttagg tgttattgct acaccttttc ccaatatacc tgttgaaaac
    12061 gctgcatctt gtatctgctc caaagccttt acttcacctt ttattgggct atatactact
    12121 tctcttttgt ttttttctat aacgccttgc ccattatcat ctttagaaat ttctactata
    12181 gcatcatctt tccaaaaaaa taatgttaaa acaaatccta ctacaatagc tactattatt
    12241 gctattatag ttgcataaac acctttcata tctccagttg ttggatttat ataattaggt
    12301 ataccaaata ttccaagtcc acccattaca taacttttta cattcattat ccccattaca
    12361 gctcctgcca cagcaccacc tacacaacta tatatgaatg gtgtttttct tggtaaagtt
    12421 attccatata ttgcaggctc tgttacacca catatccctg aaataactgc aggtatacac
    12481 agctctttta tcttcttatc ttttaattta aagtacattg ctgctactac tgctgtttgt
    12541 gcaaaagatg gtacaaatac tcccactaat attgtgtcaa accccatagt cataaggttt
    12601 gacatagcta ttggtactaa tccccaatgt agaccaaata ttacaagcac ttgccacaat
    12661 aaaccagtta atgcaccaca taaaattggt gaaaagttaa ataaagctaa aaatccattt
    12721 ccaactatat tagaagcata ggtagctata ggccctataa ccataaatcc caatggtaaa
    12781 gaaactaata aaactgccat tggtacaaag aaattcttta ctacatctgg tattatttta
    12841 gcaaacactt tctccaactt agctgctaat aatactatta aaattactgg tactactgtt
    12901 gaagtgtagt tcattgctat tactggtaat cctaagaaag ttatatatac tggtgattca
    12961 aatattgttc cagtaaatag attgtataaa ggttgacctg tagatgctag agtatctaat
    13021 tgaatacctg aatagcataa aactgcacct ataagcattc caataaatgg ttttaatttg
    13081 aatttcttag cagctgtata tccaagaatt attggcatat actggaacat tgcatctcca
    13141 attgcattta acataatata tgcaccatca gtttctccat acaaacccaa tgctgtaaat
    13201 actgcattaa atccttttat cataccagca gcacacagaa ctcctaatat tggttgaaaa
    13261 actcctgata ttatgtctat aaacttatta agtaatcctt ttggcgcatc tgagtctgaa
    13321 ttatctattg tatctcctcc taatccagcc aaatcgcaca caacttcata aacatctgga
    13381 acatgattac caataactac ttggtattga cctgcacttt tcattacagt aactatacca
    13441 tccatttttt ttagcacatc atcatttgct attgactcat cttttaattt aaaacgtagc
    13501 ctcgttatac agtgagataa actgctaata ttatcctttc ctcctacatt cgcaatgatt
    13561 tcttttgata gttgttcata ctttctcatt ttttctcctc cattttcttt ttgaaatatg
    13621 aaggtttggc caacttaatg tcaccaccca atatatataa acctaattat taggatttat
    13681 atccgactta atacttttac ttaccactct ttcaacgtga atagttaagt ataatttttc
    13741 ctcatctgtt aaatcgtagt tgtatttttt atgtataaat tcatccaact tttctatgaa
    13801 tttataagca tttttatatt taacttttat tacatttaat aaatcatctt cattattttc
    13861 atgtaaatta ttactaacaa ttcgttctgc aaaatacttt aagtgagtaa taaatctatg
    13921 ataataaact gaatcttcat caaatactat tctgtaatgg tatttaacca tatttgaaat
    13981 ttcttgtatt atttgtgtaa tctcatatat agtttttata ctttgatcca tttgtgcatt
    14041 aactatatgc agtgctataa atccagcttc atcttctgat agtctaattt caaatttttt
    14101 ttctatataa tccaatgcct ctaaccctat tttaaattca ctcttataaa atctttttat
    14161 atcccaaagt aatacatttt ttacacttac tccttcacga gctctttcta ttgcagtata
    14221 catatggtct gttaaagaaa tataaatact atcatttaat cttttcccta gtttttcttt
    14281 agcatataaa attatttcat ctgataattc catatatctt ataggaatat ctgctattag
    14341 ttctttaaat ttatcagata tatttgggtc tgaaagtttg aatatcttat caattttatc
    14401 ctttgatata tgctctcctg tacgtttttt ataagctaaa cctctcccca taactactat
    14461 ttccttatga ttttcatcag ttgtaataat tacattatta ttgagtattt tttcaataat
    14521 cattgtgatg ccccctttat tagcattaag ttaaaataaa tcttttttaa ttatttagaa
    14581 tatttaaaat gtaatttatt acataaaaaa aaacctattt attacacatt aaatagactc
    14641 ccatgagtcc tgagtaataa ataggtttag cttgtattta aacaatcact atccgtttgt
    14701 attttatttt aattattatt tattatatgt tagcaaattt attttttagt gtcaactttt
    14761 tctcaatatt ttaattttaa ataaattata taaattgaat cttcaaagta ctatttttat
    14821 caataaacaa aataaaaaag gtattacttt ctttaagtaa tacccattta tttctaattt
    14881 aagtggtttt tcttaatctc atcaagtagt ccatcttcta aataaatttc taatgcacat
    14941 cctaccattg cctttactgc tttgtgaagt ttatcataag cttcctcaga atttgcataa
    15001 tttagaaatt ctttttcatg aacatatacc attggactta tatctaaggc tatttctgta
    15061 tacatagtag ggcaaacttg acttacatta cctatatctg tagaacctac tggaccatct
    15121 ttaccttcta atatatctgt tatacctgcc tcaatcaaat tattctttgt tatcttttga
    15181 agtactttgt tatttattat gttgtcaaaa gaattttcaa agtatctata agaaagctta
    15241 gctcctgtca ttaactctgc accttttgca cagtttataa cttttttagt tacttcatct
    15301 aaataacttc tttcagcagc tcttacgaaa aacttacaag ctgctttttc tggaactata
    15361 tttggagcct ctcctccatt tgaaactata ccatgtattc taacatctga ttttacatgt
    15421 tgtcttagtg catttatccc agcaaaagta agttgtactg catctagtgc attgactccc
    15481 atatgagggt gactagctgc atgtgctgat accccttcaa aactaaactc taatgaatct
    15541 atcgccaaag ccttacagtt tatattatta aaagcttcta tatgcatttg ataacttaca
    15601 tcaacatcat caaaagctcc tgccttaact aaatcacatt ttcctcctgt tgtttcttca
    15661 gctggtgttc ctattagtac aatttttcct ttaaaatttt cttttaactt acttaatact
    15721 atacaagctc ctagtgtaga tgctgcaatc cagttatgtc cacaagcatg agctggttct
    15781 ttattttctc catatccagg aagtgcatca tactcagcta aaaatgctat ggtaggtcca
    15841 tcattatctc ctaattcagc tctaaaagca gtctccatat tacaatatgg ataaactgta
    15901 tcaaatccat attccttcat tttttctact aagtattttg atgatatgta ttcttcttcc
    15961 cctaattcag gattgttaaa tataaaatta caaacttctt caatttcttt tccaagttca
    16021 ttctctatct cataagctct cttctttata ctctctatca taatttacct cctactagaa
    16081 tcctagatat aatcctattg tcaaaaatgc cattacaaga attgttaata ctaccattaa
    16141 aggaattacg aatctaaccc actttgacca aggaatattg gctactgcta gtgctcccat
    16201 taaaacacct gatgtaggtg ttatcaagtt agttacacct ataccaaatt gataagctgt
    16261 aactatcatt tgtcttgata catctactaa gtcgccaagt ggtgcaagta caggtattgt
    16321 aagtgatgca agccctgatg ctgatggtat taaaaatgct attaatcctt ctattaagaa
    16381 agttaaattt ataaatatag tttttggtaa tccatttaat aaatttgctg cagaatttaa
    16441 aattgtatct attataaatc cattttctgc tattattaca attccacgtg caaatgctat
    16501 acacaatcct gctgtcatta aatctcctgc tccacttata aatgaattaa ctatatcatc
    16561 ttgtttaaga cctgatgcta tacctgatat tatacctata gctgtaaata tcattgcaat
    16621 ttcagttata taccatccca actttaaaac tccatatatc ataatcgcca tacctattgt
    16681 gaaaattgct attaccatgg cttctctttt tgtaaattcc tttatatttg tatcactatt
    16741 aaccattgat tcttgattta gtgatatatc gtgtactaaa gattttttag ggtctttttt
    16801 aactttattg gcatacatca taacaaatcc tatagatata gccatatata ttataaacat
    16861 aacaactcta aactctattc cagaacctgg tgctatttgt gataatgctt gtgctattcc
    16921 tacagagaaa ggattcgtag tagctgctcc aaatccagct gttgctccta taaatactgt
    16981 ggccactgct gtcaatgaat catatcccat actggtcata agtggaataa atatcatata
    17041 aaaaggtagt gtctcttcac tcattccaaa cgtcgtccca cctagtccaa atagaatcat
    17101 tgaaatcggt attattaaaa attgaaactt cttcatttta tttacagtat gagctataac
    17161 agcttctata gctcctgttt tattaactat cccaaaagct cctccaacta ttaaaacaaa
    17221 tccaactact tcagcagcat ctataaaccc gtttataggt gctgtaaata catcagtaat
    17281 tccttgtgga tttgatggtg ctttttcata agttccagct acaacaactg accttccgtc
    17341 tatatctact ctttcaaaat ttccacttgg cactatccaa gtaagaattg ccattaacac
    17401 tattagcaaa aatattattg taagtgtctg tggcatagta aactttttct tttttaaact
    17461 gctcattttt aatctcccca ctttttaaat taaatatttt attattgcat ccctttatcc
    17521 tattttgtat agatgtaaaa aagctcaaca tggatacatg ttgagctaaa tgtgctaaat
    17581 acaacatacc tgcatcttcc ctttatcttg tttaagatag ctctccatca aaattcggga
    17641 taattttgat gacagtactg tacctattaa atacagtccc agcactagct tcggacacta
    17701 gcacttcggc acttactcct tttatattgc ctcattggtt tccaacctct tcaatattaa
    17761 tcttagtaaa tgcaacctct acctcaatct gtttgttaga tgaggtaatt cttattaatt
    17821 tttattaatt caaagtaatt gtagcataat atgtcgagat tgtaaatatt ttcagtataa
    17881 taattcttac tttatgattt cattccattt ttcacaaata attttttctc catataattt
    17941 aaatactgta gtatatttta aatacacaca aactttccac aaattttaaa ttattataat
    18001 acctttataa taaaaaataa aagctatgta aacttatcaa tttacacagc tttatataca
    18061 ttaatctact ccaagtacag ctttaagctt tgatgctatt gtaataactc gtggaccaaa
    18121 tataacttga actgagtttt tctttttaac aactccactt gcacccaatt catttaccca
    18181 gaagccatct tcatagaatc catcaattga atttagctct accctaagtc tagttgcaca
    18241 gtttgtaaca ttttttatat tattacttcc tcctaaacct tctattatag aatgtacaag
    18301 ctttgtatct ttatcatcta cttcctcttc tactgcattg ttgtatgctg ttttaccagc
    18361 atctacagat tgtctttctc tatattcttt cttactataa agcttaactt cttctgaact
    18421 tcttcctggt gtttttatat caaacttctt gattgcccat gtaaatatta tataagtaac
    18481 tataaaaaat attggtatca aaaataataa tggtaacgca tccacttttt gtggttgtaa
    18541 taaattagga aacatatttc taaatgattc tcccattata gaaacattaa acatttctgt
    18601 aaatacatac gtcaatcctg ccagtggtac atatgctaca aaatacaata atggtgcaac
    18661 aaataggaat gtatactcta ttggttccgt tacccctacc aacatggctg ttactattgc
    18721 tggtatcaat atacttattg cttttttctt attagcatta tcagccaact tatacatagc
    18781 taaagctgct gctggtaata ttgcaaaatg tattaataaa cgacctgttg tgaagttacg
    18841 agttatataa gatagtgtat ctgggtctcc catttgagct aatcttatat tattaacacc
    18901 ctcgtaaatt gctccaccta cttccattgt tccacctact cttgtatatt ctatagggaa
    18961 tgcaattaag tgatgtatac caaatggtaa taatgcttta tccattgctc caaaagtaaa
    19021 tgttcctaat aatccactag aacctatcca attaccaagt ttagataatg ccaaccctac
    19081 tggtggccat ataaagtaca ttgctgtacc caatactatt gatgctataa acatcataat
    19141 cattgaaaat cttggacctg agaaaaaaga taatgcacta ggaagttcta tgtcaacaaa
    19201 tttgttatgt actgcactag ctacaaatcc agctattata gctccaaata tactacaatc
    19261 atatgaaaag attcctaaga aagaaccata taaagaactt cgactatatg cttctgcctc
    19321 tcctaatcca gatgccataa acgcttctgc tgtaactgta tctgaattta taccatttgc
    19381 tgctaacaat gtacttataa ctgtgtgcat tcctataaat aaaacaattc ctcctaatgc
    19441 accccatcct ttttcctttt tagataatcc aaaggctata cctgtaacaa agaatattgg
    19501 taagtatctc attaccataa atcctaaatc gtttattact ttaaagaata catttataaa
    19561 tgacccttct tttattagtc cgctaagccc taatgcagaa acgttttcca tatttgcaaa
    19621 agctgaccct agtcctataa atactcctgc tacaacaagt aatataacag gtatcatcat
    19681 tgctccagaa agtacttata atttatcctt catagtatta tttcctccat ttttattgaa
    19741 atgccaggct atataatgca tatatatata cttctattgc tttgtataat gtatctatac
    19801 ttatattttc atttgcctga tgtgaatttg gattatcccc ctctattgtg ggtccaaatg
    19861 caactccatt ttttaacact cttgcataag atgcagctcc ttttgtaaaa agttcggctt
    19921 cttttccaaa accttgttta tatgcatttg ataatttatt tattaattca ctatctggtt
    19981 ttacatacag taattttaaa tccttatata tatcaactat taaattttct ttttttccaa
    20041 tttcattaat tctattagtt ataaactccc aacttatacc cttaggatat ctaaagtcta
    20101 ttttcatatt caattcattt ttctctaaga agatactcat tatacatatt gtagaaactc
    20161 ccatatctac atcttcttta tatatcccta atttttttcc atgattatca tctgtaaagt
    20221 atgaatttaa aatatccctt attataacgc ctttatcatt tagtttttta attttctcta
    20281 aatcttttgc taaactaaat gcacagttat atgacctgtg tggatttctc gataaagccc
    20341 tttctcctgt atatcttact aatactttac cttcttctag ttcttctatc tctgtataat
    20401 aagctaatga ttctactaaa tctttcttat cattagtttt aaaaacagcc tcaattttgt
    20461 cacatacaaa tccatcttct ttgtttgact ttatatcaac taaattgtga tttctaaatt
    20521 tatctttatc tattttcttt cttagattga agtataaaat acctttttct ccatttacta
    20581 ttgggaagtt tccatctgga gaaaatgcaa attttggctg ttcattgaca ctaaagtaat
    20641 gctccataca ttcccaagtc gtctcttcag ctccacccac tatcaatcta atctttctct
    20701 tcggcttttc attaagttct cttaagaaaa gtaatgcata taaaatccct atcaaaggtc
    20761 ctttgttgtc attaactcct ctaccataaa gatagttatc tttttgacat actttaaaag
    20821 gtttactctt ccaaagttct ttattatata ttggcaccac atctatatga ttaagcaccc
    20881 ctactacttc ttctccttct ccatattcta tatgaattgc atatccatca aagtctttta
    20941 caacaaaatc tttgtctttt gctatttgaa taagcttatc gaaggcattt cttatttcta
    21001 tcccaaatgg ttgatttata tctgttgtac tttcatctct aattgaaggt atttcaacta
    21061 aacttctaat atcatttatg atattttctt gattggattt taaataatca ataattagtt
    21121 ctttgtttac tatcatctta attatttatc acccctttat attaatttta caaagcattt
    21181 cagttcatat ctacattata aattttgaat attatttttt caatatttca aatacttctc
    21241 taagttacca taatgatacg gtaaacaatc aacagattat gacaaaatca tactatacct
    21301 attactaata ttttaactta tctattacgt cttttttaat gtaagtttat tccaaaaata
    21361 cataaaaaaa gatgtagcct tagctacacc ttcttatatc tattcaactt ccattattat
    21421 gctattaata tctacatttc caggttctat cctatctttt actgttctct taacagaatc
    21481 agcatcatcc attataacta caggagttat tatatttaaa tcatttgacg ttataaaatc
    21541 taaatctact tccattatta aatctttagc ttttacctta tctcctgcct ttaccttaac
    21601 atcaaacggc tttccttcta gtgacattgt ctctaaacct acatgtatta atatatttgt
    21661 attatcatta ctcttaatca ttattgcatg ctttgtatca aatacattga ctatttcacc
    21721 atcaacagga gaatataact taccttcact tggtattatt gctactccat ctcccatcat
    21781 cttttgggca aaaactgcat ctggaacatc ttctaagttc actaaatttc ccttaaaagg
    21841 gctatatatt ttctcaatct tattttcttt tttcttaaat atattaaaca taacacactc
    21901 tccacattat aaaattttat gataatattt ttcttaaagc ttttgctaca ccatcattta
    21961 cattagtatc agttacaaaa tctgccatag attttacttc atctgtagca tttcccatag
    22021 caataccaag acctgcaaat ttaatcatgg atatatcatt ttcattatca cctatacaaa
    22081 taacttcttt gctatctata cctaatatat cacaaaattt tttaactgca ataccttttg
    22141 aagtaccttt actcataact tcaaagttta tctttccaga acttactact tctaaatctt
    22201 cttgtttttt aaattcttcc tttgcttctc tcaaagaatc tacgttttct gagaaaagta
    22261 tacattttaa aatttcatta tcagcttttt ctaataaagt ttcacaactt gcattttcta
    22321 ttatttcaat tctgtcttct tctttatttt tactatttat taatctatat ggatgttctt
    22381 cacctatttc attgttggat ataactattt tacagccttt aaaatgtttg cttaaattat
    22441 acttttcacc tattgtatag attttcttta gagcttcttt gctcaaaacc ttcttatact
    22501 catatcccag taatttaaaa tatgtcccat tagttgatat aatatatata tcatccccta
    22561 aaacacttgt aaaatatttc atagcattat aaggtcttcc cgtaactaag gctaccttga
    22621 tacccttttc catgacttct tttagtgctt taatattttc ttctgatact tcaaatcctt
    22681 tacccatcaa agtaccatcc atatcagtac aaattaattt atatcgcatt tgtatcacat
    22741 cctagtaaga tttttctaat atttcaacaa ggtcttcttt ctttccaaaa ggtaaaaatc
    22801 ctaaaactgg acagcttgtt atttgttcta attgttcctt tgttacatta aactcagaca
    22861 tttttgtctt aagtcccact ctctttaaaa aactttctat ttcatttcct aaagttttag
    22921 ctgcttcatc atcttccact ttttctaatt cttggtttag cattctcgct actttggcaa
    22981 atttctctat attatcctta tagaactttt ctgcaaattt tgggaacact atagccaaag
    23041 cctcaccatg aggaatatgt gtaactcctc ctattatttc acttagtgga tgtggtgctg
    23101 ctgctccagc atttgccagt gaacttcctc ctaatgtatc tgctactgct aactttgttc
    23161 tgtattctat attattttta tcttccatta ctttaggtag atagttaatg actaattcca
    23221 tagatttttc agacattatc tctgtaaaat cattagcatt tggatttata taactttcaa
    23281 atgcatgagt aaaagcatca aatgctgttg atgcagtaat tctctctggc agagttaaca
    23341 taagctctgg gtcaactata cattcttttg agaaacaatt ttgatgaaat attgtatttt
    23401 tctcgcttcc tctcgatatt actgctgctt gtgtaacttg gcttcctgtt ccagaagtag
    23461 ttgatacagc tattaatggt aattcttttt cacttacctt atcgtatatt ttaaatggat
    23521 ttgtatatgt actaaacata taatcccaat ctatcttttc taaaccaaaa gtcaatgcta
    23581 atacttttgc agtatctata cttgaacctc ctccaacagc taaaacaaca tctacagatt
    23641 cttttttagc taattcaaac ccttcattta ttaattctac agttgggttt ggctctactc
    23701 tatcaaagtg tacaacttct ataccttttt ctattaaatt caatgtaact cttttatata
    23761 acttgtctaa tggctctaca tttggagtag taactaacaa acatttcttt ccatattttc
    23821 ctattattct aggtaattca cttaaagttc cttgtccaaa atttattctt gatggttgat
    23881 aaaagttaat catatttttc ctcccttaaa ttattgcctt catattcaaa ttatacatat
    23941 cataggattt ttaaggcaat accttcactg ttaactctta tttactaaag cttatggcaa
    24001 ttattagaca atcaatgttt tattattacc atatcattat gtaaatttta ttaagtaccc
    24061 atgttataat tttataatca atcttattgt ataattgact taataataaa aatagagtat
    24121 atacactttt ttatgacaaa tagcttaaag gagaattaaa ttatgaaata tttgatagac
    24181 agtgctaatc ttgatgaaat tcaaaagtgt ttaaagtatg gatttaaggg aataacagct
    24241 aatccatcta tgtatcttaa agagaaagta aacttccatg aatttattga gacatgtgct
    24301 aatttaaatc cagaaatatt aactgctgaa gtcattggaa ataatttata cgaattaatt
    24361 aattcatgtg ataagatatt aaatataaat aaaaacacta taataaaaat aaatttctct
    24421 gaagttggct tagaacttat aaacatatta aatagtaaag gtgtaaaaac tgcatgtacc
    24481 ttgattttca atgtaaatca agcatcatta gctataaatg caggtgctga ttacctattc
    24541 ccttttatag gtagaaatga tgaaaatggg aatgatggta taaaaaacct tacagaaata
    24601 tgtaacctta tatcaataaa tggatataat actaaagttg ttgcagcttc tattaaaaat
    24661 gtattccact taactcaagc atctctttgt ggatgtgatt atgcagctgt aacatatgat
    24721 ttatttaaaa aagcttcttt taaccagctt acaatagatg gtgccaatac ttttgaaaga
    24781 gattggtcca aggttaataa atagtaacta agatttagga gggtttgcat tgaacaaaag
    24841 attattagat attgctgaat tattactcaa cagctcagac tatattactg tagatactat
    24901 tgccgaagaa ttaaaagtta gtaataaaac tattcgtaat gaccttgtta ttttagatga
    24961 atggctttta gagtttaatt tatccctaga taaaaaaact ggttcagggg ttattattct
    25021 aggtaatgaa gatataaaac ttaaagttat tagagatatc aatgataaat caaattgtat
    25081 atatgcatac tctcctgaag atagaaaaag gtatattcta agtaaattat ttattaaaaa
    25141 ttctaagttt aggattcgtg atatttgcaa tgaactacat gttagtagag ctactgtgca
    25201 taaagacttg gtagttattc aaaatttttt gcaaaacttt aaagtgactc tagttagaaa
    25261 aaccaataat ggagtatatt tagagggaaa agagaaagat attcgaaaag caatttttga
    25321 acttgctacg gttaataaaa gttatactga acttaaagaa atattattct cgaaatctaa
    25381 tgaatgcaaa gatacccctt caacaaaaat atttaaggag ctatttaact atgattttga
    25441 gaaattatct caaatcactc tacacacctt acaagtagaa aaaagtaacc ttagtgatga
    25501 gtattatata aacttcttaa tacatatagc tatatgtatt aaaagaattt caattaaaag
    25561 atatattaac ttatctgaag gtttttttag agaattatta gaacatgaat actttgaaaa
    25621 atcaaaaatt ttatgtgcta acctaagtga agcttttgac gtagaattta ttgatgaaga
    25681 ggtctgttat atccttcttc atatagaagg attacttaaa tcaataaaga aaactactac
    25741 tcttgaacct cttaaaaatg aagaaattac agagtataat ctatctgaag caatagctgt
    25801 tcattgggga agtatactaa atttaaactt aaaggatgat ttaatattaa taaagtctct
    25861 tgctaatcac cttaaatcag ttctacacag aataaactat gggtttacta ttacaaatcc
    25921 catattaaat gatataaaga gaacatttcc ttatacttat aaggctgcta aagagtctaa
    25981 tactgttatc aaacagttga tgaattatga agtcaatgat gacgaaatag gatatcttgc
    26041 tttatattta atatcagcaa tagatagaag caaatctccc cttaatacta ttttaatatg
    26101 tcactgtagt gatggtgtat ctaatttatt agtccaaaag ttaagttttg aatttaatca
    26161 gattaatata gttaaatcta tacctctatc ttctatttca tttacgaact ttgatgatgt
    26221 ggacttaata ctaaccactg ctcctgtaga ctttgaacat aatgctgaac tgataaatat
    26281 aaatgctctt ttaagtaaga atgatatttc tagacttagt acaattataa aaaaactata
    26341 ttctgaaaaa aataaaatat tatcttataa ataaacaaaa ataggcttgt ctcaacatag
    26401 agataaacct atttttcatt gtctaaatga taaaaatata tttgatttca tagaaaaagt
    26461 gcctcatgaa ctaaaaaatt cattttgaga cacctccatt ttttatattt aagcttttta
    26521 tttaagcttt tatatttttt aaacttttat atttaaagta aattttatta tttttattga
    26581 gcacaagcat tatttatatt attaatttct tcagtattct taccaaactt tgtagttaca
    26641 aatcctattg tttttattat gagtaataaa gatatcaatg aatatataga tatcataaaa
    26701 ttcttactta ttattccaga cgctactatt ataaatccat ctagcataaa taatataact
    26761 ggcaacttca gaaacttaat taccttatta ataagcattg caactaaatc agttccacca
    26821 gtactacagc cccttattat tgctatccct atagcagttc ctaccaataa cccacctatc
    26881 actcctgcta ctaatagatt gtcagttatg tgagtttgtg gtattacttt taagaataat
    26941 gggcccataa gagttatata taatgttttt attccaaaac ctttccctaa aaataataat
    27001 cccattacta aaagtggcat agatattcct aatgatatat tctcaacagg cattgataag
    27061 aatgtgctaa gtacgactga taatcctgtt atccctcctg gagctaatgt atgttctgca
    27121 aagaaaaaat taactgctaa tgccattaaa aatgctgata ttgttatcag taaataatct
    27181 tttatcattt cttttttcat ttaataacct cctatgatta tattatttca gtataaaaaa
    27241 tcaattaaat tagtctagct tcctgtagtt tttcataaaa ttaatgcttc cttaaaacat
    27301 taagaaagca tctcttttat catagtttat tatcttattc taaaccaaac tttgtagctg
    27361 ctttatctat agcttttcta caattcttaa cacctgctgt tataccatct ttatccttga
    27421 atattgctga agataatatt acaacgtctg gattacattc tattaatggc tccatattgt
    27481 ctgctcttag tccaccatct attgctaatt cacacttagg attcttttcg tctatcattt
    27541 ttctagctct ttttactaaa tcaacagcac tctttctcca tccccagtta tcttttccat
    27601 cagtttcatc aacaccatga acgactattt gcaatctatc tatatcatat atagcttcat
    27661 caacaaaaca taatggtgtg taacatccaa ttgttaatcc tactttcata ccaaattctc
    27721 tacagtaatt tattatgtat gcaagaggag cacctaaaaa atgttctgct ggtattatca
    27781 acaagttaca tcctgctgct gctatttttt ctataaataa tctatcacaa tctcttgtgt
    27841 atatatggca ttctattggt ttatctgtca ctggtcttat tgcttctatt atttgatgac
    27901 cacccataag ttgcatattt tttaagtcat gcatatctgc tgcatctgaa tgcacgtagt
    27961 ctacacctgc atccattgct tctttaacta cggctgatat atgaccgtaa tttacatgtg
    28021 ctaatcctgc tgctattttt atgtgtttca tatttgtata cctccatatt ttactttaat
    28081 tagtattaaa tattagtttt tgatattttt ttaatatttt ttaatccagt attactttta
    28141 tttagcatta cttttattgc tgtatatatt tcttttatga acttattctg cttttgttaa
    28201 ctgtgaactt attttgcttt gtaaattaat attacactga tatatttttt ttcacaacac
    28261 tttgactact ccttaaaagt acctttatat tgtggtaaat tatagacatt aattgtatat
    28321 aaaaagttag ttttttacca cattcttatg gcatttattg tctatactaa ctcaattgat
    28381 atagctagtt catttaatat ataatcagac tataaatata aatttactcg ataaaaagta
    28441 ccattcataa caatattaat gaaagggaga tgtaaaatga gaaactgtat tattgcccaa
    28501 tctggaggcc ctacagctgt tatcaatgct tctgcagttg gtatttatga aaaaaattta
    28561 gaaactaaat actttgataa agtatatttt ggtatcaatg ggattgaagg tatattaaac
    28621 aaaaatataa tagattcaga ctccttaaac aaagataatg tattaaattt aaagcaaaca
    28681 ccttcgtctg gtcttggttc ttgtagatac aaactggctg attataaaat aaattcttat
    28741 gattatgata gagttataaa tatactagat gaatatgaaa tagatacatt attttatata
    28801 ggcggaaatg attctatgga cacagtgatg aaattttctc aatactgcaa aaacactact
    28861 tgtaaaataa aatttatagg tattcctaaa acaatagata atgatttatt aggaactgac
    28921 aactgtcctg gatttggttc tgctgcaaag cttatttcta cactaataat ggaaacatac
    28981 ttagattttt ctatatacaa aaataatggt atatttatag tagaaactat gggtagagat
    29041 actggatggt tagcagcttc ttcttcacta gctagactta atgatactcc acttgttgat
    29101 ttaatatacc ttccagaaat tgatttctat gaagataagt ttatagaaga tgttagaaaa
    29161 atatttaaag aaaaaaataa ggcttatata gttgcgtcag aaggtataag ggataaatac
    29221 ggcaacttta tatgcgaaca aaaaacttct gctcatgata aatttggtca ttcccaatta
    29281 ggtggagtaa gcaactatct aaagaatctt atagtatcat catcaattac aacaagagta
    29341 aaaaccttag aacttggtgt aatacaaaga tgtgctatgc atttagcttc agatactgat
    29401 ttaaaacaag cagctgaagt tggtgcatat gggatagaac tatctaaaaa aggatataca
    29461 ggagttatgg ctatcattaa tagaacatgc aataatcctt ataagtatga agttagtcat
    29521 actgatattt ctaaaatagc taacaagaca aaatattttc ctaaagagtg gataaatgaa
    29581 gaatgtaatt atataactga tgaagcccta aattatctat atccacttat tcaaggtaaa
    29641 ccagatatca aatataaaaa tggactacct tatttttcaa ttatttacta ggaaattatt
    29701 ataatttgat atacactcaa ttctatcgct cttaaatacc taagtggctg ttaaataaaa
    29761 ttataaattg ttatatgaca gtcacttaat cgtttaatta tttaactgtt tagtttttta
    29821 attttttaat cttttttaat actaatacga tttgttgtaa taatatctca cacccatttt
    29881 cattttggat agattataat tagttagaca ataaatattt tgtcatgata ttattatata
    29941 tagtgataca aatagaaata cttttatcat ggctagaaaa acaaagatta cacaaaggat
    30001 ttaaaaatca aacaacttca attaaaataa atggttaaaa taacttctaa atcaactact
    30061 ataaacaata aaagaacata aagtagttaa gtctacaatt ataaaattag taagactata
    30121 ataacttaaa taaaattaaa gttaacaaag actattaaag ttagcaaata ttcctctacc
    30181 taaataattg ctttttataa agagttattt attttaattg aatttaattg aaatacgatt
    30241 ttaactagaa caatagatta atgtaatatt taatataaaa taattataat aattgaaaag
    30301 gaagtaatct atgatgaaaa tagcagtcat aacaaccgaa tttttaaaag agtttgtaga
    30361 taattctata aaaaagttaa atattaatgc agaaattgaa atatatatct atagagattt
    30421 ttctcatgtt ggagatttat atttagaaat agaagatagg tttgatggat ttgcagttag
    30481 tggccccatc cccaaaaaag ctattacaaa aaaggctgga actattaaaa aacctctagt
    30541 tgattttggt actgacttac aaagttacta tgatatgttt ctaaagctta tctttaaata
    30601 taatacatta gatttttcta gagcgtactt tgacttaaca gactgggcag ataatccaaa
    30661 agatatatca tattatttat caaatggcac atttggagac ttaatggata gtattgatga
    30721 aaatgcatct aaaatgagtt tatctgacat agcaaaactt gaaaaaaaga ttgcaaaaaa
    30781 acatattaat ctatggaatg aagggaaaat cgatttttct actactcgtt ttagtagtat
    30841 tgttcctatg ttacaaaatg caggagtaaa ctttcatttc atataccctg atatagattt
    30901 attaaaaaat acttttaata acttaataaa agatattcat ttacatagaa tgaagggaaa
    30961 tcaaccaagt gtaatatata taacagttaa agatacaaat tatgatgact ctactctaga
    31021 ccaagtttat aaaatattaa atagtattaa aaaagataaa ttaaccgatt ttataattga
    31081 aaaaaatagt aattctttta aaattttcac taaatgtgaa acgattaaat cattaactaa
    31141 aaattttact gcatgttata taaaagaatc tattgaaaat agtacaaata ttaaagtatg
    31201 tgttggatat ggaattggta atgatataaa gcaagctatg tctagtgcca taagtgctaa
    31261 taaggaatct aaaataaatc atttaaatta cagtttctta gtcaacgaaa attatgagac
    31321 aataggacct ttactgtgtg ataagttact tactgtttca aataaagtaa ctccacatat
    31381 aaatacaatt gccaaaagtt ctaaattgtc tactcttact attcaaaaga ttatatctgt
    31441 tactgaaatt ctacaaagag atgaagttac tcctaaggaa ttatcttcat gtttaaatgt
    31501 aacagttaga actgctaata gatttctaag tagtctctta aagtctggaa acgctactat
    31561 attatatgaa aaacaaaact caacaaaagg tagacctgaa agagtataca aattatttat
    31621 atcagaaaat aaatattaaa atccaaaatg cataaaaata ttttatcatc taatattaag
    31681 taaaattata tatgactttc taaaaggtat atctttgcat ctttttaaga tatacctttt
    31741 ataatattta ataatatttt atgttattat ctactaaaat tattttctaa ccattttact
    31801 gcacaatgag caaataaagc tgaaccataa actaatccct cttcattaaa aattgcttta
    31861 ggattatgca taccaaaatt atatccctga ctaatacttc ccattccaat ccataaaaat
    31921 actcctggaa cccttagtaa aatctctgaa aaatcttctg aacccattgc agatggaaag
    31981 tctattacat tttcatttcc tataacttct ttaacataag atacaatctc atctcctaca
    32041 tccaaatcat ttataactgc aggagtcccc attttaaatt ccactttagc atttcctcca
    32101 aatgatttag ctattcctgt agatatttca ataattctat tttttaattt ttctctaaca
    32161 gattggtcaa atgttcttaa agtacctgac atagatgctt tatttggtat agcatttggt
    32221 acgcttcctg cattaaacat accaatagtt aaaactacag gctctttctg aggtttttct
    32281 cttgaaagta tagtgtttaa tgctatttgt atatgtgaca ttatgtttat tgggtccact
    32341 ccaaattcag ggcttgctcc atgagttgct tttccttcta ctgttatttc aaatatatct
    32401 gcagatgcca tagctggacc acgtgaataa gctacttgac ctgtgttaag aaatttaccc
    32461 accatactgt gaaatgccat agctgcatcg acctgaggat tttctaatat accatcatca
    32521 atcattgatt ttgcacctct ggtagtctct tcatctggtt caaatacaaa ctttactcta
    32581 cctttaagta agtgcttatt atccataagt attttagctg ctcctaagag cattgctgtg
    32641 tgattatcgt gtccacaact atgagatgca tctttattct tacttttaaa aggtaaatcc
    32701 gtctcctcca taataggaag tgcatccata tctgccctta ataataaggt ctttccacca
    32761 tttttactgt ctaaaacagc tactattcca ttattagacg tcaatttcgg agaatatccc
    32821 attttttcta aagtactggt aacaaaggtt actgttttcg gtagattgca accaatttcg
    32881 ggattttcat gtaaatatct tctccatcta ataatttccc cttctattga ctgcgcttct
    32941 ttaaaaaaat ttttcatatt ccttcctcct aatcagatag tcagcaaaat ctcactttct
    33001 tttagtaatg aaatgatgct gatataaaaa ttataatttt atatattaaa tttcatgtct
    33061 ttttaaggag ttggaatatc atctggaata ggacatttat atgttttacc attcatagat
    33121 ttatcaaatt cttcttttgc ctttttagct atctctggat tatttaatat tttagctcca
    33181 aacatagcca taatctttga tgcatatatc ataccttttt ctcctataga acttcctgca
    33241 caagatgtaa actgccagct atgacctggt gctcctagat tcgtacaccc tgtaaaaaag
    33301 tatgctgtcg gaactatatg cattacatct ccaatatcag tagagccata actattaaag
    33361 tttgtaacat ttcctccacc ataatataga tgagttcctt ctggcaaatt atatttactt
    33421 atcatagcct tatattgttg tgggctttgt ctatctaatt cagctgcaaa atccaattcc
    33481 tctcgagtcc aaggctcttg tttaatctca ttcatacatt ctgatacaag atttgctaaa
    33541 acatgattgt ttaaagtatt ataacaccct cctaaaaact ctacttcaac ttcagtttca
    33601 gtcatcattg cagctccttt tgctaccttt acaagtcttt catatgtact ttctacagct
    33661 tctctactta aagctcttgt ataataccat actgatgctt tatcaggaac tatgttgggt
    33721 gctactcctc catctgtaat tgtataatga atccttacat cagaagtaac atgttctctc
    33781 aaataattag ctcccacatt agtaagttcc acagcatcta gtgcacttct tccattttga
    33841 gggtctgagc ctgcatgtgc agtctttccc ttaaagtgaa actttacaga gttaacagct
    33901 gttgaagtac caattgtagc ttcactaatt ttatttggat gaaaatttat agctaagtct
    33961 aacccttcaa acgctccacc tctagccata aatcctttac ctgttaattg ttcttctcct
    34021 ggacatgcat aaaatactat agtcccactt aagttatttt ctatcatctc tcttttaagc
    34081 cctactactg cccctacatg tgctgtacat aataaattat gcccacatcc atgaccatat
    34141 gcacccaatt caaatgcttc ttgttttgtt gaaacttttt gatttaaacc aggtaatgca
    34201 tcaaattctc ccataaaacc tataactggt ttacctgaac caaaactagc tctaatagcc
    34261 gtggctaaac ctcctacacc tacttccaca tcaaatcctt cacttcttag aaagtttgct
    34321 gtattttcac atgctttaaa ttctttaaat gctttttctg gattttccca tattttctta
    34381 cttaaatcat caagtttgaa tcttttttta tctatctcat ttatagatat acttcctatc
    34441 attttctatt cctcctataa atctatttac tatcaaccaa atataattgg acctattata
    34501 ctagcaaatg ccactgatgc aatggttaca gtaacaaatc ctcctataat catctttggt
    34561 aataaataat ctgttgccct ttgtttttct tcttcagttc cttccatagc atcaatacct
    34621 tcattggaaa ttatctgtgt tgcaggataa gctagcatac aagttacacc aattgcagct
    34681 gacattcttg gagaatatcc caatagtttt cctacaattg cagtaaatag tattataaat
    34741 acagagccta caacaagtat tcctatagtt ggaataatca tcccaaacaa tgctgttgga
    34801 gttaccttag caagtcctcc aggtaacatc aacatacaac caaacattat aattccatta
    34861 gattgagatt tatctaaaat tcctttttct aaaaaaccta ttcgtgtaaa aatcaaccct
    34921 actaacaaac atgctatgtt gggatttaaa taatagtttg caggggtaaa tcctggtatt
    34981 aaagtagcca atcctacaaa attaccaatc atagcaacaa atgaaagctt tgcaagatat
    35041 actaaagtag atgatttttt acctaaatct tctttaaaaa ttctcatact tggaagttta
    35101 aaattacttt ttgactcatc tttcaaaaaa tccccgttat ctcttttaaa ttttaattct
    35161 ttatttatac aatatgtagt tatcggaatg ccaaagaact tttgaaatga taatactaga
    35221 attacaaatg cagcaagttc aggtctattt gcagcttctg caacagttgt tacgataata
    35281 ccagcaacag tagaacccgc tattggaggt gctgctatta aagcatactc tctaccaaat
    35341 aacatactac ccactgttaa acagccaacc cctattccta caatacctac taatgcaatt
    35401 acaaccgttt tccattcatg acataagtct tcaagattta taagtgttcc taagttagtt
    35461 attaataaag ctataccaaa gttgctcatt gcagctaata tccctgatgc tcctgtaatg
    35521 tcttttggta aaatatttgt ccaaaatcct agtaaaaata taatacatac aaataacaat
    35581 gttgaaattg caccttttgt tttcttagat aaaaactcac taaaagcata aatcattaca
    35641 catacagtaa agcaagtaag tggattccaa aaattttcca ttgttgtttc ctcctaaata
    35701 taataatttt ttatcaataa aacaataatt ataaaatgta tattatgcag ttattcttaa
    35761 ctacatatgg tatagcttga tattttttga tgatattata tacgtcctaa attattccta
    35821 aaatatctaa ataaatataa cta
//
LOCUS       DACULQ010000018        35086 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.018, whole genome shotgun sequence.
ACCESSION   DACULQ010000018 DACULQ010000000
VERSION     DACULQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.018"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(1..477)
                     /locus_tag="JA154_03830"
     CDS             complement(1..477)
                     /locus_tag="JA154_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994988.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGDHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(676..2301)
                     /locus_tag="JA154_03835"
     CDS             complement(676..2301)
                     /locus_tag="JA154_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7994989.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(2639..3658)
                     /locus_tag="JA154_03840"
     CDS             complement(2639..3658)
                     /locus_tag="JA154_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HAT7994990.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(3655..4233)
                     /locus_tag="JA154_03845"
     CDS             complement(3655..4233)
                     /locus_tag="JA154_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HAT7994991.1"
                     /translation="MIYMINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKID
                     FHQVVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQ
                     PFIDNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTIL
                     KNSSKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            complement(4331..5053)
                     /locus_tag="JA154_03850"
     CDS             complement(4331..5053)
                     /locus_tag="JA154_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HAT7994992.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLVENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            complement(5236..6513)
                     /locus_tag="JA154_03855"
     CDS             complement(5236..6513)
                     /locus_tag="JA154_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT7994993.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            complement(6652..8958)
                     /locus_tag="JA154_03860"
     CDS             complement(6652..8958)
                     /locus_tag="JA154_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidase"
                     /protein_id="HAT7994994.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            complement(8951..9745)
                     /locus_tag="JA154_03865"
     CDS             complement(8951..9745)
                     /locus_tag="JA154_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAT7994995.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            complement(9852..11210)
                     /locus_tag="JA154_03870"
     CDS             complement(9852..11210)
                     /locus_tag="JA154_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease"
                     /protein_id="HAT7994996.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGSSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            complement(11665..11742)
                     /locus_tag="JA154_03875"
     CDS             complement(11665..11742)
                     /locus_tag="JA154_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994997.1"
                     /translation="MDIWSPSLEIGRSNDYLEREYTISD"
     gene            complement(12558..13565)
                     /locus_tag="JA154_03880"
     CDS             complement(12558..13565)
                     /locus_tag="JA154_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7994998.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEITVMKEDMSEVKQ
                     EINVLKVDMSEVKQEMTVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEM
                     TVMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNF
                     MQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLL
                     HNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSV
                     K"
     gene            complement(13795..14880)
                     /locus_tag="JA154_03885"
     CDS             complement(13795..14880)
                     /locus_tag="JA154_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAT7994999.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(14916..16229)
                     /locus_tag="JA154_03890"
     CDS             complement(14916..16229)
                     /locus_tag="JA154_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7995000.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLNENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDTKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(16230..18521)
                     /locus_tag="JA154_03895"
     CDS             complement(16230..18521)
                     /locus_tag="JA154_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidase"
                     /protein_id="HAT7995001.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGSDKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(18511..19305)
                     /locus_tag="JA154_03900"
     CDS             complement(18511..19305)
                     /locus_tag="JA154_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAT7995002.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(19344..19790)
                     /locus_tag="JA154_03905"
     CDS             complement(19344..19790)
                     /locus_tag="JA154_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HAT7995003.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(19862..21268)
                     /locus_tag="JA154_03910"
     CDS             complement(19862..21268)
                     /locus_tag="JA154_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7995004.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(21286..22656)
                     /locus_tag="JA154_03915"
     CDS             complement(21286..22656)
                     /locus_tag="JA154_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HAT7995005.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEEIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(22789..23976)
                     /locus_tag="JA154_03920"
     CDS             complement(22789..23976)
                     /locus_tag="JA154_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HAT7995006.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            complement(24103..25317)
                     /locus_tag="JA154_03925"
     CDS             complement(24103..25317)
                     /locus_tag="JA154_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HAT7995007.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            complement(25680..26351)
                     /locus_tag="JA154_03930"
     CDS             complement(25680..26351)
                     /locus_tag="JA154_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT7995008.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(26434..28650)
                     /locus_tag="JA154_03935"
     CDS             complement(26434..28650)
                     /locus_tag="JA154_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase"
                     /protein_id="HAT7995009.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(28653..29123)
                     /locus_tag="JA154_03940"
     CDS             complement(28653..29123)
                     /locus_tag="JA154_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HAT7995010.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(29185..30579)
                     /locus_tag="JA154_03945"
     CDS             complement(29185..30579)
                     /locus_tag="JA154_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7995011.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(30652..32013)
                     /locus_tag="JA154_03950"
     CDS             complement(30652..32013)
                     /locus_tag="JA154_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease"
                     /protein_id="HAT7995012.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTAIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            complement(32310..33659)
                     /locus_tag="JA154_03955"
     CDS             complement(32310..33659)
                     /locus_tag="JA154_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAT7995013.1"
                     /translation="MSKYQKNVSMFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVV
                     GGAVGVSPTQITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVG
                     GNYGLASILGAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMA
                     GGVNSPTYASMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIIN
                     FDPIREATWFAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNIT
                     DKELSSGVIGSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSG
                     FVPKFGALMTTIPYSVLGGATISIFSIITMTGIKLIITEELSSRNITIVGLAIALGMG
                     ITSVPAAQEAFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            complement(33988..35028)
                     /locus_tag="JA154_03960"
     CDS             complement(33988..35028)
                     /locus_tag="JA154_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT7995014.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT        10623 a         6910 c         3790 g        13763 t
ORIGIN      
        1 ttatatactt gctaacattg gaatatctaa catcttcttc attacatcat ctactttttt
       61 tgctttatat tcattgtatc caattaaata tcctttttca tatgaacttt ttaaaatctc
      121 tttttcatat aaattatcag atttggcttc tgtgacactt aaattataaa gccctttaca
      181 cccatcttca taacctttat tatacccttt ttctgcaaaa tagtttttat gatttttttc
      241 gttatgtact gaagatattg ttactccaga attttctttt gtactagcat aaggacaaaa
      301 acctttattg tgttgatgtg ctgaatgtcc tccacaataa taacgtgtta actctgattt
      361 atttttatta tgatgatctc ctgatgaatt atgccctcct gagtgagcat atgaaaaatt
      421 ccctacacta atcatagcta aactaattac tacagctact aaccttttct taaccataat
      481 ctattccccc caaaatgtta tatattttta ttatatcatc tactatatta tatttaaatt
      541 cttaatatac aaaatattac tctattctgc taataaacat gtttaaacaa cataaaaaac
      601 ctcctaatat ttgattaaaa atttacacac tcatgcatag catgtctcaa atattaaaag
      661 gttttatact aataattatt tatcttttcc atacatttta gctaattggt cttgtatctc
      721 tttactttct aaatactcat caaagtccat ttttctatcc attattccat ttggagttat
      781 ctctataatt ctatttgcta tagtagatat aaattcatgg tcatgagaag taaataaaag
      841 cactcctggg aacttttcaa gtcctttatt tacagaagtt atactttcta ggtctaagtg
      901 gttggtaggg tcatctaata caagtacatt tgcatttgaa agcattagct tactaagcat
      961 acatctaacc ttttctcctc cagatataac ttgagcttct tttaatgctt cttcacctga
     1021 gaataacatt ctacctaaaa atcctcttat aaaactttca cttttttctt cagagaactg
     1081 tctaagccag tcaaccaaag aatattctac tccgtcaaaa aacttattat gattttttgg
     1141 aagataatct tgagaagtag ttactcccca tttatactct ccactgtctg gttcaagttc
     1201 tcccataact atgttgaaaa gtgcagtagt agctatttca tctcccataa atactacttt
     1261 atcatctcta tccaatctaa atgacacatt atcaagaacc ttaaccccat ctatagtttt
     1321 agttaaattg tgtacttcta aaacttcatt cccaatttct cttgcaggtg taaatcctac
     1381 atatggatat cttcttcttg atggttgtat atcttcaact tctaatttct ctaactgttt
     1441 ctttcttgat gttgcttgtt tagcctttga agcattagaa ctaaatctag ctataaattc
     1501 ctttaattga gctatctttt cttcagtctt tttattttgg tccttagcca gttgtaaagc
     1561 caattgactt gactcatacc agaaatcata gtttccaacg tacatttgta ttttaccaaa
     1621 atctacgtca actatattag tacatatttg atttaggaaa tgtctatcat gtgatactat
     1681 tataactata gattcttcta aatccattat aaaattattt aaccagttta ttgatttaaa
     1741 atctaagtgg tttgtaggtt cgtccattaa tagtatttca ggcttaccaa aaagtgattg
     1801 tgctaacaat accttaacct tttccccacc aactagttcc ttcatttgtt tgtaatgcat
     1861 atctttcgtt atgccaagac ccataagtat tttctcagca ttagtttctg catcccatcc
     1921 gtcaagctct gcaaactcac cttcaagttc ggcagctttt atcccatctt cttcattaaa
     1981 gtcttccttc atatacaatg catctttttc tttcattatg ttccaaagtc tctcatgacc
     2041 tcttataaca acatttaaaa cagtttcttc ttcatattca aagtggtcct gttttaaaac
     2101 tgacattctt tctttatcag ttatagatat actacctgta ttaggctcta tttctcctga
     2161 taatattttt aaaaaagtag attttcccgc accattagct cctattattc catagcaatt
     2221 acctttagtg aattttaagt taacatcttt aaatagttcc ttatcaccaa atctaagtcc
     2281 aacacctgta acttgtaaca ttaatatatt ttcctttctt tttgctcata atttatttta
     2341 tcataaaact ttgataacat ttttattata tattatcacg taaatagtat attttttata
     2401 ctaaatttgt gaaatattta ttaaaataat actaaaaatt gtaaatttat aaaaattaac
     2461 aatctcttat attatactta taaagaaatc aaattgcaac tttattcatt cttttacaca
     2521 ttgaaaataa gttattataa tttctaaata aatatgcttt tcttaaaaca tcattctagc
     2581 tgttttgttt catatttaaa aagagtactt aatttgtaaa tactcttttt taataacttt
     2641 atattttagc aaaattacat gcaggataag tacatacatc acagttcata caaaggccac
     2701 catgaccata tctagctata tcatacttat caagcttttc atcaattaaa accctagata
     2761 aaactatatc aaatactgta gttttgtgat acattgcgca tcctggtata cccataattg
     2821 gaacttctcc ataataagca agtaaaaaca tagctcctgg taatattggt gaaccatatg
     2881 tcactaaatc agctccagtt tcttttatag ctgttggtgt aacatcatct gggtctacac
     2941 tcatcccacc agtacaacag ataagttctg ctccttgact tataaattct tttattgcac
     3001 tctttataat ttctttatca tctgggcata tagtctgtcc tataacttca caaccaaagc
     3061 cttttacttt ttcttctatt acgggtccaa atttatcaac aattctgcta taaaacactt
     3121 catttcctgt agtaacaatt cctactttct taggtttaaa aggtactaca tttactattt
     3181 ttctattacc aactactttt tttgcatctt ctaatttttt ctcatctata actaaaggta
     3241 tcactcttgt tccagctact tttaaacctt tatttactat aaaattatta tgaagagtag
     3301 ccatcattat ttctccaaca caattcaaat caaaaagtgc atcaacatct atctttaata
     3361 gaccatcaca tgctgctata aaatctattt tcccttcttt tatttcacta aaatctaaat
     3421 tttctccagc agttagttct tttaaaaaca atgcgccttc attttcatga accattcctt
     3481 ctgacttttc ccatacatac aaattatctt taccaagaga taataacact ggaatatctt
     3541 cttcttttac tatgtggccc tttttaaatt ttcttccttt aaattctcca ggtattattt
     3601 gagttatgtc gtgacataaa acttgtccaa cagcatctat agtttttatt agcttcataa
     3661 tttaccccca attctctcat aatccatttt tgtatcgata tcaaataaca tttctttagg
     3721 aacttctaca tactttattt ttgatgaatt ttttaaaatg gttttacctt tttcatcttc
     3781 tttaagtgcc aacaattctt ctttcttact atatgggaat atgactggat ttccacacat
     3841 atctttgtat cttggaatta ctataaaatc tctatctaat ttaaaagtat ttataatttt
     3901 atctatatat aagttatcaa taaatggctg gtctcctaca aagaacatat aaccatcaca
     3961 ttttgagcta tttaaaactc ctaattttat agattcactt tgacctatat ttgcattttt
     4021 attatctata tattcaaaac catatttatt acacaataac aaaacctctt catattgact
     4081 tactactaca acttgatgaa aatcaatctt gctaacttct ctaaatacat gttctattat
     4141 agaacagcct ttataattta ataaaagttt atttgctccc attcttctgg caaagcctga
     4201 tgccattatc actgcattta tcatatatat catccttggt atttataata tattgatagt
     4261 tctatgattt ttataatatc tctattgata ttatctatat atataatatc atataaacta
     4321 ttttaatact tcaatatctt acacaaaatt tttgttttac acttccataa aatatctcta
     4381 tttcactttc atattcttta atattttcta ctaattttat agctaaattt ttatattttt
     4441 cattttcaac tttattaata aataatactc tttttccaga ataattttta aataatccat
     4501 ttggatttaa tactatattt tttaagtttt caatacttac tgtagaacta ctattaatac
     4561 tacttgtatc taaatttgct atttttttaa atatttctac tctatgtata ttctcttcat
     4621 taatattcat tccaaatgat gttatatcta atatccctat ggtttttgta gttttatgat
     4681 atactactgg ttctttagca ttccaacctt taagtttttt tctctttgag ccatctccct
     4741 ctattaaaac taaatcaaat tggtctacaa tttcatccaa aatagaggaa tcaatactta
     4801 ctaatttatt ttcattattt atataactgc cacagactgt tattccatga caaatagatg
     4861 gaataacagt tccatctatt gtaataatta tattgtcata atcgtttggt acatatattt
     4921 tagttgttgt tgtaaggaga actttcaatt tatctctata aaaatttgca aaatagttta
     4981 taaacgaagt ttttcctcct gctccaacta cagttattat ttcattcttt tctattatat
     5041 ttgatagttc cacactttca cctcttataa ccagttttct aatctcttaa ttattatagt
     5101 tagacctttt tagtaaaagt gatttttgaa attccataaa ctcgaaagta aatagattct
     5161 gttgtaaaca acaaggcgga tccaattcgc cgctaagctg taagcttacg ctatcagttt
     5221 atcttttaaa tataattaag atattcttac tctctgtggt tgctttaata tttgaggatt
     5281 attctgtaaa ttagataaat taactacttg tgattttttt agtatattaa gactcgcatt
     5341 tacatctgca tttataatga ttccaacact ggatttgtac tgtccacgcg ttattctttc
     5401 ccctttaaag tcatattttt taacattgtc cacattgata tcaggtagaa aatcatcagc
     5461 aaaaaaatct gatttactag tatagctttc ttcttgccta ataaaattga tattttttat
     5521 tttacacaga tattctaatt ttccttttat ctctccaaat gggattctta taaaatttct
     5581 atatattttt ctctccatat tatcactttt ttctaactta gcattatatc ctacaattaa
     5641 atttccaata ttatttttta cacaataatc aattatataa aaacaggttt tatttacata
     5701 gtctttaact tgattatttc tcttattcca aactttaaat tgttttcttg ttattgcagt
     5761 tatattcttt tttaatttta aggcttctat tttatcattc tgtttgtttg cccactgatt
     5821 cacagatttt agcctttttc catctatgat aaaactatca cctaaattgg taacacatgt
     5881 acataaattt tctaaaccca agtctatagc tagtgcattt ttttgatttg aaacttcctc
     5941 tgattttggt atttcataac aatactgaat ttcaaagaac ctagctttat gtttaggtat
     6001 tattcttatt tctttaattt ttttctctag tatattttgt ggaactttta tacttacttt
     6061 cccatatagt tttttaaatg ctggagacat aggtacattt aaaatcccat cttctttgat
     6121 tcttatttga cctattatta tgctaaaaaa tccttcttta tctaaatatt taggtgattt
     6181 tatccctcta aagtcatatt ttccttcttt ggctaattta attaatccaa aaaatgacct
     6241 aaacgaactg tctacttctt ttaatatttg ttgcgctata ttactattga gaagcttata
     6301 gttctcattt tctttgcata catgataatt agcttcatag ttaagatgtt ctccttgatt
     6361 gaaataatgc tgtcttacat tatatgtacc aacattatat aagttcttag ctaagaagca
     6421 catctctcgt agagcttgat attctttttt agttaagttt tttaattgtt gtctctgagt
     6481 tccatagtgt atatttttta ttctgtttga catgaactca cctcctttag aatatactct
     6541 actagacatt atatacggta tttttacaaa acaccatata taatgtccat ttaattactt
     6601 tattttacaa aataatttaa atttacttgc ttttatacac attattattt attataattg
     6661 tttattatta tttttcatac ccatataaac tttttcaaaa gtcataggaa gctctcttaa
     6721 acgaactcct gtagcattaa atattgcatt tgctatagct ggtgctggtg tatgtataac
     6781 tatttctcct aatgatttag caccataagg acctcctggg tcgaaattct cagcaaaatc
     6841 taccaatata tttccaactt ctttttttgt aggaacttta tactgtagga aactatctgt
     6901 taataatcta ccatctttat caaatatagg gtcttcatac attgcaaacc ctatacccat
     6961 catcacacca ccttctactt gtattctagc taaagttgga tttataactg ttccacaatc
     7021 aggtacacat gcaaaattta tgactttata ttcgcctgta tttttatcta attctatctc
     7081 tgcaaatcct gctataaatg gttttatttt tttcaacatg ccaaaagatt ctgttgcttc
     7141 caaaactact ggtacatcat cactagcaaa tcctattcct ccaacagatt ctctacccaa
     7201 ttcctctaat agaacaaact tgctattatc gtttttaaca aaaaccctgt cttttcccaa
     7261 ctctaaatct tcttttttaa atcctagctt tgactctgca actgaaagta atatatcttt
     7321 taatttattt gctgtcttta ttgttgcatt tcctgtaacc gttgtcgtac aagaagcaaa
     7381 tgctcctgta tcatagggac ataagtctga atcacctgta taaatagaaa ttctatctac
     7441 agtagtattt aaagcttccg cagcaatttg agcttgaatt gtatcagccc cttgacctaa
     7501 gtcagcaaga ccagataata gtttatatgt gccatcttca tccattctta tagtgactgt
     7561 tgccatatct acacgcggta taccagaacc atgatttgct actgccatac ctactgctct
     7621 tactttgttg tttcctatat ctttacatgg atatttatca tcccaattaa ttaatttctt
     7681 gcccttttct atacattgtc tcagctcaca acttaatatt tcgtcttcag ctgcttttcc
     7741 attggtatac ctatctatta tatttttaat tctaaattca agtggatcca ttccaatctt
     7801 atgtgccaat tcatcaatag cactatctat tgcaaatatt ccctgtgtag caccataccc
     7861 tctaagtgct cctgctggta acctattagt atatactgtt ctagcattaa atctaacagc
     7921 atcaatatca ttatatattg gtaaggtatt gtgtccagat tcggaagaaa cagagacacc
     7981 attcccacca taagcacctg tattgtttaa tgactttagc tctattgctt ttaaatttcc
     8041 ttctttatct gcaccaactt taacatcaaa aaacatttca tgtctactat tagaacctac
     8101 aaaagtttca cttcttgtaa atacaaattt tgctggtcta ccagttttca tagtaacaaa
     8161 tgcagtaaat atatctgttg ctgctatatt ctttcctcca aatccaccac ctattctagg
     8221 tttaattact cttatttttg aagatggtat acccaatact tttctaagta ttctctttac
     8281 attgtgtggt gattgtaatg atgaaactac aacaattcta tcatttatat ctttatatga
     8341 atatgaccta tgagtttcca tcatgcaatg cgattgactt tgggttttgt atgaagattc
     8401 aaccacaaaa tcactttctt taagcttagc ttcaacatct ccaaattcaa aaaattcttt
     8461 tgatactata ttattttttg catccaatcc aaaaggatat ggctctacta tgtctttttc
     8521 tggatgtata acaacttcat ttccttctgc ttctcttgca tcaaacacag gctttaaaac
     8581 ctcataatct acctttatca tttttaaggc tttaagtgcc gttttctcat cttcagcagc
     8641 cactaaagct acttcatctc caataaatct aactgtatct tcaagtaaaa ctctatcata
     8701 aggtgatgct tctggttctg tttgacctgg cacacaatat ctgacctgtg gtacatcttt
     8761 atacgtataa atacccacaa cccctggaat tttttcggct tttgatgtat ctatattctt
     8821 tatcttagca tgagcatgtg gacttctaag tagttttaat gtcaaaacat ctttatgcat
     8881 aatgaggtct tcagtatata gaggttttcc tgtgacaatt gctgtactat ctatctttct
     8941 tatcccctta ttaatcatta tacattccct cctcaatagc attatacatc cttttaacta
     9001 atgcacttgc catatccttt ctgtactctt tactagctct catattactt ccataagtaa
     9061 gttccttact agcaacaata gatgccttat ctatatcttt ttctttattt aatatattac
     9121 tcgcttcaag tgctaatcta gctttttgag gtcttgcccc aattgctatt ttgtaagtat
     9181 cattttcttt tagcattgct gaatttaata cagaaaaatc tcctgtgcat tttcttattg
     9241 agtcaaaaac agctattgca tctttttttg gtagtattac ttcgaccaat atatccctac
     9301 ttaattcact atccaaaaac tcacttaact ctagtattcc tttattgtat aatctcacct
     9361 tcgctccaac aaccaaaagc gctggtatta agtcagaaaa gccatacttt gaaaatacac
     9421 ttgcaccaat tctagcccct tgtctaaatt gaacaccaac tatattagaa acagcgcttg
     9481 aaagtattcc actacagtaa ttttttatag ttttatcaat ttctaaactt ctaagtgaag
     9541 tatctgcacc tatcaaaata ttttttttat cctcttttat ataatttaga tttatatctt
     9601 ttaaatctat cgctgttcct atatttttat tacttaattt tataaatcca catccaccta
     9661 aaataacatt atttttttta gaaattaaaa ttttatatgc ttcttctaaa ctctgtggaa
     9721 ctgaatattc ctttatagta accatcttat ccccccatca ttaacttatt aaaacacaaa
     9781 agtttttaat aaatctttta tttatattta taattgtagt atatttaatt cacatatatt
     9841 aaacctttac attataaact tttattttta tcctcaacct tatcactttt atttccagga
     9901 aattttacca tatataaagc ttgaattatt atggctacca ttgctcctga aactacactt
     9961 gaatctgcaa gtaatgtctc tataaatttt ggaaattttt caagaacttg aggagctacc
    10021 atgaatccaa taccagaagc catagaaaga cctaatatca tgctattttc ttgattaaat
    10081 ccatccatac taacaagtct taaaccagat gttgtaagag ttgaaaatat aacgagtgtt
    10141 gctcctccaa ccacacaggg aggaattgtt cttattaaat tagctaataa aggcgatatt
    10201 cctgccaaaa ataatccaat tcctccaata gctagtacaa atttacttac aacctttgta
    10261 ttggaaacga ttgcagaatt ttgtccaaac atccccattg gagttgcaga aaataatgca
    10321 cctattgtag agagtactcc atttcctaaa acagcagaag aaagttcttc atctgttact
    10381 tgtctattaa gccctcctac cgcagaaagt gtacaagcac ccattatatc agctatctca
    10441 actaaatata ttattgaaaa cattacaaca acttcaagtc ttatatctag cccaaaagca
    10501 agtggttttg gaagtgcaac caaagtaaac tcctgaattg aagaaaaatt tactataccc
    10561 aaaattaatg ataaaatgta tccaaaaatt atgctaacta atattgatgc atctttaaat
    10621 aatccttttc catacttatt taatcctaat atcataagag atacagatat tccaactata
    10681 aaattaattg gattcccaaa agtctcattg cccatacccc cagctaagtt ttttatagct
    10741 attggaaaaa gtccaagacc catacatgca actattgttc ctgatacagc tggagtaaat
    10801 ataaatcgta tttttttaag accaaaacca attataatta ttactatacc agctattatt
    10861 tgagagccaa ataatactgg tagtccatac ttacttgcaa cagatacccc agctcctata
    10921 aacatagctg tagctcccat tataactggt attcctcctc ctaatttaaa acctttaaac
    10981 aaaggtattg gatataattg taataaagtt gatgatcctg caaaaactaa tgcggcttga
    11041 atcaataatg ttgacatctt atgattaagt ccagctgcat ttgcaatcaa tatcgctggt
    11101 aaacaactac ctattaccat tgccattaag tgttgtaacc ctaaaggtat tgcttccttt
    11161 agtggtggta ttccgtctaa ttcaaatttt gatgtacttt ttgctttcat aataaaaact
    11221 cccgttacat aaaacttatt gaattatata aatacttaaa aatctaatat attaaattaa
    11281 tgatgttcat aaatatcaac tatactccaa catatattta agtttttagt cttaaaaaac
    11341 agatgcaata aatttaatta ttaacaaata aacgtattct ttaattttgt atacattata
    11401 taccatgctt tacaaatata agagtattat agctatgaat ttttaatttt ttatagtaaa
    11461 tttaattcaa tttaaatctt tcaatcttaa tcattataag ttataagttt tcataagtat
    11521 ttacatgttt caagttttaa acatttacct ataaacgcta ctttatagat gtcacttcaa
    11581 atccagtgac ctcatacttt catatgagcg tagactattt cttcatcttc agcattacct
    11641 gctaagagca acccgtttcc actatcaatc gcttatagtg tactctctct cgagatagtc
    11701 gtttgacctt cctatttcta ggcttggcga ccaaatgtcc atttttaagt acttaggctc
    11761 tcaccatata ccatcctcac tattgtttct ggttttcacc acattcataa aagtttcttt
    11821 ctatctttta ttgtagttgc aaggctttag gatttactgg ttttaaagtt gtgtcctaca
    11881 tagatttctc tatgtacggg gccaaaagta caatttattt aacatacttt gctttacccc
    11941 ctgtaaataa tattatttac tctttcactg tttttgttat taaattaatt aaatatcaat
    12001 taatctaagc ataaaataat tatttaattt aaactcaaaa tatttatgaa tattttataa
    12061 tataattctc ataagacaga aaaacttaat aagctatgta tctcagttgt accaatgtac
    12121 tctcctttac tatatttgta tctgcaacat aaaatataca agtttctatc tatttataga
    12181 tatatatatc tataagcaaa atagtaatat tatttttaat agtaaaaatt ttattcatat
    12241 tactatttat aatttttata gagcaatttt agtgccaata tttgcataca tttgtatagt
    12301 ataaaatttt tatgttctca ttgataaaat tttacttatt tcaagcgttc tattagattt
    12361 tatcatctaa attttaggtt ttatttttat aaattaattg tatttacata aaaaaaaaga
    12421 cctaaaaaca aatttttaac ggtcttttct caatttttta ttttcatttc taaaatttat
    12481 aataaatagt aagaacacta taaattcttt tatataaaat tcttagtttg tggatgataa
    12541 attagtgaat taaaatacta ctttactgat tttagtttga caatatcact ccaattttta
    12601 ctagtggcta actctactaa atttaagtct cttcttattt cagctatatc ctcttttata
    12661 gaattagtgt caccttttat atatgctatg ttatgttcta tattatcatg agtagcttta
    12721 tttatttcag aattatgtaa taaacttcct aaaatattat tctgttctat ctgtatctct
    12781 ttcattgatt tatcttcaca ttctagacta tcaatcttaa tttcaattcc atataatttt
    12841 tcatttgcta tggatacctc gtccttaatt ccattcatat cttcattaat gttatttatt
    12901 ttattttgca taaagtttac ttctgattta acttcggaaa catcttcttt cattacagtt
    12961 atttcttgtc tcacttctga catgtctttt ttcattacat ttatctcttg ttttacctct
    13021 gatgtatctt ctttcattac agtcatttct tgtcttactt cggacatatc ttctttcatt
    13081 acagtcattt cttgtcttac ttccgacata tcctctttca ttgtatttat ctcttgtctt
    13141 acctcagaca tatcctcttt cattatattt atctcttgtc ttacctcgga catatcttct
    13201 ttcattacag tcatttcctg ttttacttcg gacatatcta cttttaatac atttatttcc
    13261 tgttttactt cggacatatc ttctttcatt acagttattt cttgttttac ttccgacata
    13321 tcttctttca ttacagtcat ttcttgtttt acttccgaca tatctccttt taatacattt
    13381 atttcctgtt ttacttccga catatcttct ttcattacag tcatttcttg ttttacttcc
    13441 gacatatctt cttttaacac atttatttct tgttttactt ccgacatatc attttttatg
    13501 ctaactacat cttcatttat agtatataat aactctaaaa tcttctcttg cacttgcatc
    13561 accatcccta ttaattatta taacatttta cacaaatgct atacaatgtt aacatatata
    13621 tcagaattat taaaatttta attaaacaaa attgattcac tttattactt caaaactaat
    13681 taatatctaa aataactaca aataaacaaa aataaggtca acaacaaaaa aggcttatct
    13741 ctttgagata gcctttctta tttttattta aaactttgtt ttttataaaa taatctatac
    13801 attcagctct gaacatgaaa tttttcttcc aagtgctttt ggtttacaaa ctgatataca
    13861 aagtccacaa tatctacact cttcttcatt caattccatc ttatcattca attttctcgc
    13921 ttgatatgca caaactctct cgcacatctt acatttagta catttttctt catcataagt
    13981 aaactcaaag ctaagtcttt cattaacatc tcccatatga ttatgtacta taccacaagc
    14041 attttctata gtattatatc ctaatctatt aagattagat tttaaatcat catgtatttt
    14101 agatattact tgtgcgcctt ttaaaattac tactgaacat ataccaacaa agtcagcacc
    14161 tgccataatc atttctaagg catcatctcc acttacacat ccacctaagc caattattgg
    14221 tatattaact tgttttttta tttcatatat tctctgtaaa gttattggct tgatagtttc
    14281 tccacttaaa tatcctattc catttccacc taataaaggt tgccctgttt ctatgtctat
    14341 tctaaatgct ggtcctacag aatcacaagc agttattgca tctgctcctg cttcttctaa
    14401 ttttttagca aaatcgccta tctcatctat cataggagga agtttaacta ttacaggtat
    14461 atttactctt tcctttgcat ctttaagcat tggtatcaaa tctctatagt cataagatac
    14521 taattcaata aagtcagctc ctgcatttgc aactttctct actaattcgc tactttcttc
    14581 aattgtatga cctactgaag ctatacagac agtcccatct cttttcattt gtggtatttc
    14641 aaaatctatc cattgctctg gctcaaaatc tgtccatttt tcattattta aaagagcatt
    14701 agctgtattt ctaaccatat gtggtgctgg atttatagct ctttcttttc ttatagtctt
    14761 agtcacaact gcccctgctc ctgcatcact taatatcttg catccagctg cactatatgt
    14821 taatggtcct gaacctatta taagtggtga ttttaattct ttacctaaaa aattatacat
    14881 tatctgcttc cccttttcat atatatttac attcgttaaa atttccaaaa ttcacaagca
    14941 acttctttca tttctttaac aatagctttt ttatctaaat caactaactg tttttctttt
    15001 aaaatatgtc taccatctat gtatacattt gatataaatt cttttttacc ctgaactact
    15061 atctggtcaa atatattatc aagagtcaca ggagttttaa attcatcttc cataactatt
    15121 atatcagcct tattaccaac tgctatttta ccagaatttt gaagtcctaa tacataagca
    15181 ccatgctctg ttgccattct aaatactaaa tttgctggca ttactgatgc atcttcttca
    15241 actgatttat gcattaaaaa tgcacctctc ataacttcaa aaaaatcatt tatatatccg
    15301 tcagtaccca gtgccacttt tatacctttt ttaatcattt ttggtactgg agaaaaacct
    15361 ccacctactt cacagttgct aagaggcata tgaaccactt ttacaccttt ttcttttaat
    15421 atatctattt cttcatcatt tacttttaca cactgagatg ctaatacagt ttcatccaat
    15481 atatctaaat cattatagta gtcaactgct tttttgccaa aatgtttttc agcatagttt
    15541 ggctcatata tactctcaca taaatgaaat tgcattggtg catttaattt ttttgtatct
    15601 tctttagctt tcttaataaa tctatcagaa catgtgaagc ttgtatgtgt acacattatt
    15661 ccatttatta atttactatt ttctctactc cactttatta aattagaatt ttcatttaaa
    15721 catctaagtc cattttcaaa gcttattctt tcacaacttt ctaaggataa aatcgctttc
    15781 atgcctattt cttctagtat ttttccttgc tcaattaaag ttccttcttc tgtatttgga
    15841 gcttctaggg tatcacaaaa agctaccaca ccagattcta taagttctat tgctgatgct
    15901 ttagttgttg ccttcacttc tttcaatcca attctatttt ctataaatgg ccaccaatag
    15961 tcatttaaga aaccttcaaa gtcggtaaaa tgtacatttg ccggtattcc cctagaaaga
    16021 actccatatt gatgcatatg accattgacg aatcctggca ttattatagc atctgattta
    16081 tctattattt cttcaatatt tttatatttt atttcaactt cttcatttgg caatatatct
    16141 tttataatgt cattttctac tacaacagca gaatttgtat atatttcatc agcagaagat
    16201 attaagtatt tacttttaat agcaatcatt tatttacctc cctgatttaa ttttttcatg
    16261 gccatataaa ctttctcaga agttatagga agttctctta tacgaactcc tgtggcattg
    16321 tatattgcat ttgctattgc tggtgcaggt gtatgaataa ctatctctcc taaagattta
    16381 gctccatatg gacctgttgg ctcataatta tctacaaaat caacaattat gtttcctata
    16441 tctttttttg ttggaacttt gtattgtaat agattagaag tttgaagctt tccatcagaa
    16501 ccatgtctag ggtcttcata cattgcaagc cctatacctt gagttacacc accttcaact
    16561 tgtattctag caagtgcagg attcataact gtaccacaat ctgtagaaca agcataattt
    16621 attactttaa actcccctgt tgttttatct aactctattt cagcaaatcc tgctaagaat
    16681 ggtttagcac tctcttgtat accataagat tcagatgaat ataaaacttc ttgatttcca
    16741 ccaccaacag attcttttcc taattcactt aacaaaacaa acttctctac atcttcacta
    16801 taacaaactc tgtcttcata tagtattaaa tcatctattg gtaaatcgag tttcttctgt
    16861 gctactttta atatcttttg ttttaaactt tcagcagatt ttattgtagc atttccagtt
    16921 acataagtag tacaagaagc gtatgcacca gtatcatatg gacacatatc tgtatctcct
    16981 gtataaacgg aaattctatc aatagatgta ttcaatgctt ttgcagctat ttgtgcaagt
    17041 attgtgtcag aaccagttcc taagtctgaa gacccagaaa acaacttata agttccatct
    17101 tcatccatcc tcatatttac tattgccatg tctatgttgg ctataccaga accatgagtt
    17161 cccacagaca ttccaacagc cctcacctta ttgtttccaa tatccctaac agggtacttt
    17221 tcatcccatc caataagttt tttacctcta attatacatt catcaagtgc acaacttctt
    17281 attggaaaat tcattattcc gccttcagtt tgttttttta taatattctt taactttatt
    17341 tctgttgggt ccatattaag tttatgcgct aattcatcaa ttgcacaatc aagtgcaaat
    17401 gtaccttgtg ttgctccata tcctcttaat gctcctcctg gaactaaatt agtatataca
    17461 gttctcccat caaattttat tgcaggtaca ttattatatg ttggaagtac attatgacct
    17521 gattcagagc aaactgaagg tccattatcg ccataagcgc ctgtattgtt caatgctttc
    17581 atatctatag cttttatgtt tccttcttta tctgacccaa tttttatatc aaaaaacatc
    17641 tgatgtctag tattagtcat tgcaaaagtt tcttctctag tataaattag ttttgctggt
    17701 ctttttgtca tccaagttac aaatgatgca tatatctctg ttacagctat gttttttcca
    17761 ccaaaacctc cacctattct tggttttatt actctcactt ttgaaagagg tattcccagt
    17821 gctttagaaa cctgtcttct catatgataa gctgattggt ttgcagaagt tactactaat
    17881 ctgccattcg tatctatata cgtataagct ctatgagttt ccatcataca atgggcttgt
    17941 gattgagtca tataagaatg ttctactatt acatcacttt ctttaaactc tttttctaca
    18001 tcccctttac ccattttttc tctcgataca atattttttg tagcatccaa tccaaaatca
    18061 aatggacaaa acaaatcatc ttcttcatgg attaaaattt tatttccttc cgattctctt
    18121 gcatccaata taggagtcat tacttcatat tcaactttta taagtttcat agctttaata
    18181 gcagcccttt catcttcagc ggctattata gcaacttcat ctcctacata tctaactacg
    18241 tcttcaagta ttaattggtc atgaggtgat gcttctggat atgactctcc aaccattgaa
    18301 tatctataat ttggaacatc tttgtaagtg aatatgttta ctacaccttt tacctttaat
    18361 gcatttttta tatctatgtt ttttatcttc gcaaaagcat gtggacttct aagaagctta
    18421 attgttaaaa catctttatg aaaaattaaa tcttctgtat atattggttt tccagtaaca
    18481 atggcttcac tatctatttt tcttacctct ttatttacca tcacactcac ctccaatact
    18541 attatacatt ctcactacta aggcttttgc catatctttt ctgtattctt tacttcctct
    18601 tatattactt ccaaatgaaa gttcttcact tgatacttca ccagcttttt ctatatcatt
    18661 ttctaaactt aatatttcac ttgctttata ggcaattttt gctcttcttg gtctagcgcc
    18721 tatcgcaatt ttataaatac ttccttcttt taacattgct ccatttaata ctgcaaaatc
    18781 acctgtacat tttcttatag aatcaaatac acctatagca tcttttttag gtaatataac
    18841 ttctattaat atatcttttt caagctcatt ttctaaaaaa tcagctaagt tcataacccc
    18901 ttttttatat aacttaacct ttgcatctac cactagtaat gaaggtatta aatctgaaaa
    18961 tccatactta gcaaatacgc tagctcctac tctagctcca cttctaaatt gaactcctac
    19021 tatattagaa acagcgtttg aaattacccc attacagtaa tttttaataa ccttatttaa
    19081 ttctaaagtt cttaaacttg tatctgctcc aattaatatg ctatcatcag tttcatttat
    19141 ataatctaaa gctaagtctt ttaaatctat tgctgaacca atatttttac tacctagttt
    19201 taaaaatcca catcctccaa gtattatgtt gttttttctt gatattagca gttcataagc
    19261 ttcatctaaa ctttttggaa ctacatattc ctttatagtt atcatactct ctccccatat
    19321 caacttaatt ttcacttaaa agtctaagtg tttaaatatt tatgtattgc tcttaattga
    19381 cctttatatc ctgtacatct acaaaggtta ccatttacat aatctataac ttcttcttct
    19441 gttggatttt gaagctctct tttcattgcc aagaccatca ttataaatcc aggagcacaa
    19501 aatccacact ggtcagcccc ttcgtctgca agaaaatctc caaacttctt tgcttcatcc
    19561 aagacaccct caagtgttgt aatatgctga ccttctactc tagcagtaag ataattacat
    19621 gaaagcacgg ccttgtcatt taccatcact gtacataacc cacatgagcc tgtatcacac
    19681 ccttttttaa cacttacata tccattttct cttaatgtat ctgctaaaaa atcatctggt
    19741 cttatactta attctttttc tttattattt atggtacatt tgataatcaa ataaatcacc
    19801 tcaaaactca tatttttaag ctaaagtttt actatacttt tttatgaata aattacattt
    19861 attaataaag gctattccat ttatgacctt ccattctatt ttttatagtt cctcttacac
    19921 aaagtctctc agctgtatct tgagcatctt ttaatgcttt ctcttcatta gctgtcaata
    19981 tttttgaatc ttccattatt atatttccat ctactataac actctctata tttttcatac
    20041 tagaagaata tactaatgta gataccggat tatgcatagg tatcgctttt ggtgaaagca
    20101 ttggattaaa tattaataag tctgcttttt tacctatctc caatgaacct atctcatctt
    20161 ccattccaat agctcttgct ccatctatag ttgccaactc aagaactttt tctgctgaca
    20221 ttgctaatgg gtcacactta ttaactttat gttgaagtgc tgttaatttc ataagttcaa
    20281 gcatatcttg agagttatta ctagcggctc catctactcc taaactaact gttattcctt
    20341 ttttaagcat ttctggcact ggtgcaacac ctgaagataa gtacatatta cttgctgtat
    20401 tatgagacac tttcatatcg tattttttag taagttccat atctttttca gttagataca
    20461 cacaatgtac cataagcaca tttggtccaa gtattcctaa tttttctaaa acatctatgt
    20521 catattgccc atgcaattct tttgctgctt ctctatcaaa aggagtctct gatatatgta
    20581 ctgtaaataa agcatcatca tattctttta ctactctcca tagcatttca agcatttctt
    20641 gagaatttga ccatattgct gcaggagcta ctcctatttt gattcttcca ttttctgtat
    20701 tatggtgttt ttcaaacaat cttcttacat ctttttcaac cgtttctaca tcttgcatta
    20761 ttccaggatg aactccaaat tgagctcctg tattcataca tcctcttcca agtattcctc
    20821 ttattcctaa ttctttataa gcatctatta ttccatcaca aagacctggc ttactatgag
    20881 gatacatgta gtcaaccatt gttgttatac cacttctaag tccttcaata catccaagca
    20941 ttgcagcatc ataagtatct tttggctcta aatagttagc tgctggaaat gtcatagttt
    21001 ctaaccagtc tttaagtacc atatcatctc ccaatccttt taataaagtt tggaataaat
    21061 gattatgtgt atttataaaa cctgggaata aaactttacc tttagcatct ataatttttt
    21121 taacatcagt atatttactt tctatctctt tagaatttcc aatatcagca atcttgttat
    21181 cttttactac taatgcccca tcaaaaataa cttctctctc tttattcaca gttactatta
    21241 tggcattctt aataagtata tccatcattc ttatttcccc ccgcattatt tagtaatttt
    21301 tcttttaata tattgacctg ttggatgttc tttaacatta caactttgta ctacatgatt
    21361 tcctctcaaa aatacatctt caactttaca tttaacctta aatccttcat atggagtata
    21421 atctacattt tgtttttgag ttttataact aattgtttct tctttattga tatttaaaat
    21481 gactatatct gcatcacttc ctacagctat ctcacctttt ttaggataca ttccaaatat
    21541 ttttgcagca ttcgtagaag taacttccac aaacttattt gcacttattc tattttctaa
    21601 gactccatat gtatataaaa gtgctaacct atgttcaaca ccaggtgctc catttggtat
    21661 cttactaaaa tcatcaattc ccaagtcttt ttgcccctta aaattaaatg aacagtgatc
    21721 tgtgccaact gtttgaatat ctccactaga caatccttcc caaaggtaat ttatgtcttc
    21781 tttttttctt aaaggaggag acattacata cttagcaccc tcaaatttat ccttattata
    21841 taaattgtct tctaataata gatactgtgg gcatgtttct actatcatat ctatattttt
    21901 ttctctagcc atttttacag tttcaagtga ctccctacaa cttaagtgga ctacatagga
    21961 ttttgaatta gataatttag ttatatcagc aagtcttgaa acagactctt tctcaactat
    22021 gttaggtctt gtaagtggat gatattttgg ctccaaatta ccattttgta tattttcttc
    22081 tatcaataca tctagtaaat ccccattttc acaatgaaat ccaactatac aacctaaatc
    22141 ccttgctttc tttaaaaccc tgtatattgt tccatcatct actttcatgc catcatatgc
    22201 catatacatc ttaaaagata ctatcccatc cgaaataagt ttttccatat gattaaatgt
    22261 atctttattt aaacatgtaa ttgtcatatg aaaaccataa tcacaataac aatttccact
    22321 tgctttttta tgatatgctt ctacaccttg cagtaggttt tccccctcat tggcttctgc
    22381 aaaatctagt attgtagtag ttcctccagc tatagctgct cttgttcctg tttcaaaatt
    22441 atctgcagtt gtaatagaac ctgcattcat atcaaaatga gtatgtgtgt ctattccacc
    22501 aggaattaca attttacctg tagcatcaat aaccttatca ccatttattt ctaagttatt
    22561 tcctatttca actatctctt catcttctat ccttaaatca cttatataag tcttatcaga
    22621 ggtaattata gttccacctc ttagtattgt tcccatatat acctcctaac ttttaagctt
    22681 taaagtattt tctaagtatt gtaaaacttt atttaaaatt gtattttcta aaggtgctat
    22741 attcagcacc tctagactat atttaaataa tttattgtga actaaatatt attttccgta
    22801 tacagatttt aatattccat agtaaccttc tgttcctata aataactgtt cttgctctat
    22861 atactcatct attgtgtgag caagattttc cttagaagga ccaaatccta tagtttttat
    22921 tccagcttca cctgcatagt gacttccatt cgtacagaat gagtattgag ttatttctga
    22981 atctattcca gcttctttta atcccttata tgctgcttga acaaattcat cttcttcgtc
    23041 atataaccaa cctgggaaga atctttcact ttctatagta tttcctgtat agcaatcagc
    23101 tttttcaact gcataagaaa cttttgcatt taattctggg tcttctttca tcatttcatc
    23161 taataaagct tgtattggag ctaatacgcc ttctcttgtt tctccaacta ataatcttct
    23221 atcaaaagta gctttgcaat aatctggcac tactgatgct cctggatatg gagaagattt
    23281 tatatctgtt aaaactaata ttccatcacc taaaactgga tgagttggag gtactaattg
    23341 ttgtatcttt tggattacat tagccatttt ataaactgca tttacacctt tttctggatt
    23401 agcagagtga gctggtttac caaaagtttc tactactatt tctcctctac ctctctgacc
    23461 aatctttaag tttaactctg aagactcacc tattacaaca tagtcaggtt gaacagcctt
    23521 acttatctct ctagccgaaa caccttcaaa tatttcttca tgaacaacac ctgctacata
    23581 tagttctcca gcaaaatctc tattagtgtc tttagcaaaa tacgctacag cagacatcat
    23641 tgcagtatat tgacctttca tgtcagaagt tcctctacca tatatctttc catcaactat
    23701 ttttccttca aaaggaggaa cactccattt acttgcatct ggaactggaa ctgtatctat
    23761 atgtccatca aataatattt tcttacctgg cttgttacct tttatacatc ctactatatt
    23821 tccatatcta tctcttgacc aactatcaaa acctaatttt tcaaaagact cttctattgc
    23881 ttttacaaca ccttcttcat ttccagaaga actaggattt tgaattaatt tttggcatat
    23941 ttcagttact tcttgttttc tttgttcgtt taacatttca ttatccccct agatttttta
    24001 taatgtatgt attttatata cttatatatt ttctaaattt tagaacttat atatcttcta
    24061 aatatgtaaa ccaatatcta ataattgcat tttagtaatt ccttatatac ttggatattc
    24121 tccatcccaa actatacttc tatatttgtc agggtcagta tctccttctg tgcttattaa
    24181 aagtattctt gaattttcat ctaattttaa atcttttctt aaatctgcta aatcttctct
    24241 ttccatagct gctactaaaa gaccagttgt aactgctcca gattctccag aaattacctt
    24301 ttcatcacca tttaaaggat tacctaatgt tctcataccc ttagcagaaa cccagtctgg
    24361 tgctgaaaca aatgctgctg catgattttt taatacttca aatcctatag tatttggctc
    24421 tccacaagca agccctgcca ttatagttgg catatctcct ccaacaaatc ttggttttcc
    24481 atctccagct tctgctgatt tatagtaaca atcagcttca tcagcctcta ctactactgt
    24541 tattgggcat tcatcatata ttgaagcaac atacccttga actgcaccag caagtgaacc
    24601 aactcctgct tgaacaaata cgtgtgttgg tctatctact ccatattctt ttaattgctc
    24661 tatagcttct tgagccatag ttccatatcc ttgcattatc cacgctggta tttcttcata
    24721 tccgtcccat gctgtatctt gaaccattac tccatggtca tcagcttctg cataatcatt
    24781 agctaatctt actgcatcat catagtttaa gtctgttata ctagcatctg caccttctcc
    24841 tttaatttta tttaatctaa attcagaaga accttttggc attaatacaa cagatttttg
    24901 acctaattta ttagctgtcc aagcaactcc tctaccgtga tttccatctg tagctgtaaa
    24961 gaaagttatt tctcctaatt tttcttttat ttcttttgaa gttaatttgt cataccctaa
    25021 ctcagatata tctgtatcta atctttgagc taagtatcta cccatagaat aagaaccacc
    25081 taatacttta aatgcattta atccaaatct ataagattca tcttttacat atactcctgc
    25141 aacacctaat ttttttgcta aaccttctag gtttgctaat ggagtttctt tatattgagg
    25201 aaagcttttg tgaaaatttc taaccttagt tatttcttct tcatttaaaa atttgataca
    25261 attttcctta tcgccctttg gcaaattatt tactttccat ttaatttctt ttaacatcta
    25321 aaatacctcc aaattattgt taaattttta tactttaata tataagcaat taacatgcca
    25381 acactaaata tatactagat atttttctat gactctttag tatagaaatt gcaatgcttt
    25441 tattattttt tcttttctat ctttagttca tacaatgtat gtagaattta ataactaaaa
    25501 aaccttttct atcttctcac tttttaattc tcatttttaa aatttaaaaa taaatctcaa
    25561 aacactttgt ttctatattt tttaatataa aatctataaa atctaaatta aaacataaaa
    25621 tagtgatgca ataatctatt acatcactat ttgtatatat tcaataatat aataaatctt
    25681 tagtggtctc taagaggtga tttttcatct tttttaacat ctaattcttc ttttacgaca
    25741 ttccattttt caagaccaaa cttagaaacc ataactgcct ctgctgcacc ttttccaagt
    25801 ctttttactg tgtccatacc tgtctcatcc tcttgggcac cttttgcttt tccatcttta
    25861 ctccatattg taccacctat atagcatcca cctaaacctc cagacactaa catttcatgc
    25921 attaggaaga aatttattat aggaagtttt gtcaattctt ggccaccata tctagttcca
    25981 ccagtagtta tagctgcacc aaccttattt tgaagttttc ctggatgaac taaatacatt
    26041 ggacgaagtc tattaaatac tgctgtaagt tgagcagtta tattcatatc ataaactgga
    26101 gaacctacta taattccatc tgcctccaat attttttgct ctaattcttg gatatcatct
    26161 tttataatac acatagtttc ttttcttata catgcatcac aatgtacaca aggacttatt
    26221 tttttccctc taactgacca atactcagtt tctataccag gtatagtttc tgcttctttc
    26281 aaagcttcta acactgcata ttctgttgct cctttacgtg gacttccaca aatacctaat
    26341 atctttgcca taataatatc tcctcaccaa ataaatctat ttttattatc tatttacaaa
    26401 tacaataaat tgttatttct gctttaattt atactatttt aattttattg gtaatgaatg
    26461 tatctctaca tcagttgcat tatatactgc atttgcaaca gctggcgcaa ctggtacaac
    26521 ggcacattct ccaatactct taccaccaaa aggcccagct ttttcataac catctacaaa
    26581 tgcaacttga cattttggca tatctacagt tctaaataat ttatatgttt taaaatcagt
    26641 agctttaggt ctaccttgtt catcaaatgc caattcttca ctcaatgcgt atcctagacc
    26701 catatgaatc ccaccttcta attgtccttc taaatttaga ggatttataa ctctaccaac
    26761 atcatgtaca gctacaaaat caactacttt tatatcctta gtttctttat ctactaaaac
    26821 ttcagcaaaa tgtacaccat aagaagtacg tccagctggt gaagaataat cttcaacaac
    26881 agttaactct cttaatgatt tactttgact atatactgcg acatcagaaa gtgatgcttt
    26941 tacattttca tcaagtttac taattacaca tccattgtct agatataaat cttcttttga
    27001 tgtttcaagt aatttttctg cttcttctaa tataagattt ttcaatttct ctgctgtctt
    27061 tttagctcct ccaccttcta caaatacccc tctacttgca tagtcaccaa ggttgaatgt
    27121 acatgcatcc gtatcagttt caaatgtatc aactttatca ggtgttattc ctaaaacttc
    27181 agctaccatc attgtttgca tagttacaac accattaccc atatcatgag ctcctgttag
    27241 cagtgtcaac gtaccatctt catttaattt aagagtcaat gtaacaacat ctctatgtgc
    27301 accaaaaact ccattaccat gtgcacctat tgccatacct atacctctta aatattttcc
    27361 ttcttctttt ggaagttttt tcttttcatc ccacttaaac atcttcttac cttgctctat
    27421 acaatccaat actctaggat ttccaagaga accgtggaag attatatcga catcatctgg
    27481 ctctactgca tttttatttt gaaaatcgac taaatccata cctatttcct ttgctatttt
    27541 cgcaaattga gcttgttgag ccataaatat ttgaggtgac ccatagcctc tcatagctcc
    27601 tgctattggt gtatttgtat atacaggcat cccagtaaac tttatatttg gtattttata
    27661 aactttaaat actttatgac taagtgctcc tataacattt aaagcacttg atgcatatgc
    27721 tcctgtattt gtatatattt ttatgtcttg tgctattact tcaccatctt tatttacacc
    27781 agtttttaat tttacaaaag acccatgacg agttcttgtt gatatcatag attcctttct
    27841 tgtaagaacc attttaactg gtcttcttgt catttttgat aatgcggctg ctactggttc
    27901 aattgtaagt tctaattttc caccaaatga gcctccaata gcaggtctta taactcttac
    27961 tttatttaat gacatcccaa aaatttgaga tagtattatt ctaaatccaa atgtattttg
    28021 gcaaggtgta tgtacagtta atttatcatt ataatcatag tgtgcaatta cactatgagt
    28081 ctctattgca ctatgatgta ttgctggagt agagtattca tcttcaaata tatagtcaca
    28141 ttccttgaag cctttttcta catctccccc atttatgttg ttttgagaaa ctatatttga
    28201 atctccatgt atgcagatag catcttcttt tattgcatct tgtatagtaa atacagctgg
    28261 caattcttca tattcaactt ttataagttt tacagctttc tctgctattt ctggagtctc
    28321 tgcagctact gctgcaacac ggtcaccaac aaatctaact gtatctgaaa atataacttc
    28381 attttcaggt attttgtgct cataaaatct taatgaacca ttgtattttc tttgtgatgt
    28441 attgagataa gtagctatag ctctaacacc tactactttc tcagcttcac ttgtatctat
    28501 actttttatt tttgcatgtg ctacagggct taaaagcatc ttagcgtaaa gtacattttg
    28561 tggtttcata tcagccacgt atttcatttg accagtaact tttaaagctg catcttttag
    28621 agggtatgat tttcctatta ccttttccat aattatctac cccctaatct agctgcagat
    28681 aattttacag cttctataat ctttacatat cctgtgcatc tacagagatt ttctttaaaa
    28741 gcttctctaa tttcatcttc attaggagat gcatttctat ttaaaagagc ttgtgttgtt
    28801 ataatcatac caggagtaca atatccacat tgtacagctc caacttctat aaaagcttct
    28861 tgtattggat gaagattttc tccattagca aaactttcta tagtgtatat aactttacct
    28921 tcgcagtttt ttgcaagaag tttacaagat ttcatgtcaa ctccatctat caaaacttta
    28981 catgctccac aactgccact attacaacct tgtttcgctc cagttagatt taatttttca
    29041 cgaagtacat gcaaaagagt ttcattcttt tcaacttcta tttcatacat gtaatcatta
    29101 atatttaatt ttattttttc cattttctcc tcctacagat aaactaatgt tgtaatagaa
    29161 tattattaca atcttatttt aatattagta taagctattc cacttatgtc cttccattct
    29221 attagttaca ttagctctct tacataattc ttctgctgca tgttgtgcat taagcaacac
    29281 ttgttcttca ctttcaattg tagttacttt tccattttca agtattacat ttccatcaac
    29341 taaaacactc tctacattgt gcatactagc tgagtatacc aaagttgata caggattgtg
    29401 aagaggtatt gattttggag aaagcattga gttaaatatt actaggtcag cttttttccc
    29461 aatctccaaa gaacctattt catcttccat tctaagagtt cttgctccct caattgtagc
    29521 catttcaaga actttctctg cagaaattgc cagagggtca caattattta ctttgtgttg
    29581 taaagctgtt agtttcataa gctctaccat atcttgtgcg ttattacttg ctgcaccgtc
    29641 aagacctaaa ctcacattta tacctttttt taacatttct ggcacaggtg caacaccaga
    29701 agcaagatac atattacttg ctacattatg agaaactttt atgtcatact ttttaagcat
    29761 ttccatgtct tcatcagtta tacatacaca atgtactgct acaacttctg gacctactat
    29821 accccatttt tcaagtaatt ttatatctat ttctccatgt atatcctttg ttgctgttct
    29881 tgcaaattct gtttcagata tatgaactgt aaaatatgaa tcatattctt taactatatt
    29941 ccaaagcatc ttacccattt cttcagatat agcccacatt gaagaaggag ctacacagat
    30001 ttttattctt ccattttctg aattatgata tttttcgaat acccttctca aatctttttc
    30061 aaccgtttcc acatcttcta taagctcttt gtgaatacct aaatctatac aacctctacc
    30121 tacaacccct cttatgccta aatctttata tgcctttact attccatctg taagtcctgg
    30181 tctattgtga gtatgcatat aatcaaccat tgtagttata ccacttttta aaccttccat
    30241 acatccaagc attgcagcat cataagtatc ttgttctgtt aagaattttg ctgatggaaa
    30301 catcattgta tttaaccact catgaagtgc catatcatca cctaaacctt ttaataacac
    30361 ttggaacagg tgattatgag tatttataaa gccagggaat attacttttc cattagcatc
    30421 tataactttt ttagcatcaa gatacttagg ctcgatatca actgtatttc ctatgtccaa
    30481 gattctgtca ccttgaatag ctattgctcc atcaaatata acatctctgt ctttattaac
    30541 tgtaacaatt attgcatttt ttattagtat atcaatcatc ttaaatcccc ctttgaatac
    30601 tttcattaca acattttacc atatggtttt ctcatattat atctaataca tttatccaat
    30661 attttcttca agatttgaag taacgttttc agcactttta cgtgacttta atttaaataa
    30721 tgtttgaagt aatatagcta ttattgctcc agaaactata cttgaccctc caaggacagt
    30781 tccaattgtt tctgggaatt ggtctaaaac ttgtggagca gttgtaagac ccatacttgt
    30841 tgctatagat acacctgcta taagattgtt ttcttcagtg aatccatcca tactcataat
    30901 agagaaacca gacattgcta ttgatgagaa aactactaaa gttgctccac caacaacaca
    30961 agatggtata gttgtcatta ttgcacccaa tgctggagat atccctgcta gtaataatac
    31021 aagtgcccct agtgctatta caaatctact tgtaactttg ttcatagata caattgctgt
    31081 attttggcta aatactccag ttggaagagc tgcaaatagt gatgaaatta tagaagttat
    31141 agaatttcct aatacaactg atgctaattc ttcgtcagtt acctctctat ccattgctcc
    31201 tactgtagct attgtacagg cacccatcat atcagctata cctattatgt aaataatagt
    31261 aaacattact attaactctg gtctaaattc taatccaaaa gctgctggtg ttggtaaaga
    31321 tactatagct gctccttgaa cggctgagaa atcaaccaat cctaaaacta aagatatagc
    31381 ataacctact aatattccta ttaatattga agaatttttt actaaacctt taccgtattt
    31441 atttatcatt attattacaa aagcaactat aactcctact gtgaagttta ttgggcttcc
    31501 aaatgtttgt gtgccttctc ctccagctaa attctttata gctgttgcga atagtccaag
    31561 tcccatacat gctattatag ttccagaaac tattggagtg aatatatgtc taattttctt
    31621 aactccaaat cctacaaaga atacgattac cccagcagca atctgtgctc caaatagagc
    31681 tgctaaacca tctttagctg ctactgaaag acaagctcct aaaaatacat aactcattcc
    31741 catcattaca ggtaatcttg aacctaactt aaatccttta aataaaggta ttggatataa
    31801 ctgtaaaaac gttgctaaac ctgctgctaa catagaaccc tgaatcaaca ttgttgcttg
    31861 cccctggtta agtcctacca cgtttgctat taatatcgca ggtaccatat ttcctactat
    31921 cattgctaac agatgttgta aggccaaagg tattgattcc tttagtggtg gtatcccatc
    31981 gacatcatat tttgaagtat tacgtgtttt cataaaataa atcctcccat acataaatat
    32041 cttgactaaa tatttttata attattttct ataaatattt ttatgaccat gaatttattg
    32101 ctcaaccgat aaacacagct aaactgagct atttttataa aaaattaaca aatttctaaa
    32161 tattgcctcc ccgattttca acttaaatga acattaattg cctggtatat atttatttgt
    32221 taaatttttt attaaaatga atagctatca aaaataatct aacaatctca tactaaataa
    32281 tttaaacaat tcttttaaat tattatatct taattagtct cttccataac agctctttct
    32341 ttagcctcat cctctagact tttatttggt attattagat ttaaaacgaa tgcaaccaat
    32401 gttgcaatta ctattggaga ttctccaaat actaacttta caaatgaagg aaatgcctct
    32461 tgggctgctg ggactgatgt tattcccatg cctaaggcaa ttgccaaacc aacaatggta
    32521 atatttcttg atgacaactc ttctgtaata attaacttta tacctgtcat tgttattata
    32581 ctaaatatag atattgtagc tcctcctaaa acagaatatg gtatagtagt cattaatgca
    32641 ccaaatttag gcacaaaccc cgacaataac ataaatatac cagctattcc aagcacaaat
    32701 ctacttatta ctttgttcat agctaccatc cccacgtttt ggctatatga tgatggtggt
    32761 aaacctccaa acaaagctcc tatcatagta caaattccac ttcctattac tccacttgat
    32821 aattccttat ctgtgatatt cctatccatg cctcccattg tagtagctga taagtctcca
    32881 attgcttgaa cagcattgat tatacttaca attactactg agattattac aggcatatca
    32941 aattccatcc caaattcaag cggtttaggt atcgcaaacc aagttgcctc tctaataggg
    33001 tcaaaattta ttatacccag aactaaagat acacaatatc caactataat tcccaaaaat
    33061 attgctgcca attttgcata tccttttgca aattgactaa gacctataac taccactaat
    33121 gttatagccg ctactgccca gttttgcata gaggcataag taggactatt tacaccacct
    33181 gccatataat ttattgctat aggatacaaa gataatccaa ttgtaaaaac cactgttcct
    33241 gcaactatat tcggaaaaaa ttttcttatc ttttttatga atatccctat taatactgta
    33301 gccactcccc ctattaattg tgcacccaaa atgcttgcta atccataatt tcctccgact
    33361 gctacaagtg ttggtacata agtaaaacca actccaaaaa ttataggcaa tcttgaacca
    33421 atatttccta ttggatatag ttgtataagt gttgatatac cagctattat taatgcatat
    33481 tgcactaaaa gtgttatttg tgtagggcta acacccacag ctcctcctac gactataggt
    33541 actgtaacag ttccaactat cattgctagt atatgttgca ttgatagtgg caaacacttt
    33601 aaaattggtg gccttccatc aatatcaaac atagaaacat tcttttgata tttactcaag
    33661 gttatcactc ccaactattt tttaatgaca gttattatta tctcacacaa catcctttct
    33721 ttttttattt tgtcatctca acttaatatg aagcaattat aatgccaatt ttttaacata
    33781 attgttttac atatataatt tttattatat ttgttagtat ttgataattt attaaagcat
    33841 ttattttttt aaattctttt aaattttttt tatttttgga taataaaatg ctattacaca
    33901 aaaaaacact tagaaaatag ttttccaagt gttttttatc aattttataa tttcaaattt
    33961 tagaattgag aataaaaagc ctaattatta tacatctatt tttctgtaca atgtagctag
    34021 tgatattcca agttttttgg cagccagttt tttagactta gtatcatttc cgtaaaactt
    34081 taacactttc tttatatagg ctctttcaaa ctcttcaagt gtaagtatgt cttgttccat
    34141 acttatctca tagtcacttt cctcaagtaa ctttatattt atattattta acttaaaata
    34201 ttcaagtatc tttctattta ttattccttt atgtattatt ccatcttcct caagaaggtt
    34261 taatgccagt tctatagtat tttccaactc tctaatattt cctggccaag gatacttaag
    34321 caacatttgt tccacatctg gctctatatg gtacacgttt ttcttcatct ttctcgaata
    34381 tttgtctata aaattgttta ttaatatttg tatatcgtct cctctttctc taagtggagg
    34441 cacttctaaa ggtattacat tcaatctata gtataagtct tctctaaatt tattttcctt
    34501 aaccaactca aataaatctc tattagtagc tgatatgatt ctaacatcta tatcaattag
    34561 ttcatttgaa cctattctcg taaacttctt ttcctgtaat accctcaata actttgattg
    34621 tagttgaatt ggcatatctc ctatctcatc taaaaataca acccctccat tggccaattc
    34681 aaacttacca attttacctt ttgaatctgc tccactaaaa gctccttttg tatatccaaa
    34741 aagttcactt tctagtaaag gttctggtat agcagcacag tttatagcaa cgaatgtttt
    34801 attccctctg tttcctgcga gatgaatggc tcttgctatt aattcttttc cagtgccact
    34861 ttctccagtt atagctacag tagaatcact atttccaatt ttacaaattc tattcttaag
    34921 agcacgcatt acatcagact ctccaattat ggagtctact gttgttgttc cccaaatatt
    34981 atatacttcc attccaccat acattactga atcactgtgt ttgtccataa aatatcaact
    35041 ccaaataagt attctatata ataatcattc acattatcta tttaat
//
LOCUS       DACULQ010000019        34234 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.019, whole genome shotgun sequence.
ACCESSION   DACULQ010000019 DACULQ010000000
VERSION     DACULQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.019"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1153
                     /locus_tag="JA154_03965"
     CDS             <1..1153
                     /locus_tag="JA154_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT7995015.1"
                     /translation="YIESKYTIIISKADRVYLYNHFITKSSLLDNVSNRVDTKIIEYV
                     EEILEVINNQYTFDLRNDSVLFDDLVLHFKSILNSKSYNLNKVNPLINTIKSNYPLAF
                     EITLNAIEKVFKNSIYSLTEDEIGYVSLHIGAGIERFFQSNIKCKNVVLVCGSGYGSS
                     RLLEVQLNKVFHDKINILQCLSFNQFLASELSDVDIIISTIPLNHDSIPIVLVDLKLL
                     KKDIENISKSITNNSHIYSNLLDNFFDKNLFIVNPKIKDKDELIKLMCNKLTQSEIVF
                     PSFAESVFYRESLSSTNIDDFLAIPHPMELSSIRTKICISILDEPIYWSEDSTVKLIM
                     MLAINKDDYIKINSIYDILLKIIHDNDIRDSISNCNDFDNFLSIIKSIV"
     gene            complement(1413..2174)
                     /locus_tag="JA154_03970"
     CDS             complement(1413..2174)
                     /locus_tag="JA154_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7995016.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            2393..2869
                     /locus_tag="JA154_03975"
     CDS             2393..2869
                     /locus_tag="JA154_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995017.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            2967..3119
                     /locus_tag="JA154_03980"
     CDS             2967..3119
                     /locus_tag="JA154_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HAT7995018.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(3279..3467)
                     /locus_tag="JA154_03985"
     CDS             complement(3279..3467)
                     /locus_tag="JA154_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAT7995019.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            3828..4178
                     /locus_tag="JA154_03990"
     CDS             3828..4178
                     /locus_tag="JA154_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAT7995020.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            4197..5840
                     /locus_tag="JA154_03995"
     CDS             4197..5840
                     /locus_tag="JA154_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAT7995021.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            5918..7978
                     /locus_tag="JA154_04000"
     CDS             5918..7978
                     /locus_tag="JA154_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HAT7995022.1"
                     /translation="MLDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDR
                     NNLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIK
                     NTFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIM
                     SIIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEE
                     LLILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLE
                     KIMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFG
                     INVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFG
                     VMQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEK
                     SKRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGL
                     LHGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQ
                     LRGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTR
                     QHGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKE
                     ISLN"
     gene            8037..8591
                     /locus_tag="JA154_04005"
     CDS             8037..8591
                     /locus_tag="JA154_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAT7995023.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            8593..9090
                     /gene="coaD"
                     /locus_tag="JA154_04010"
     CDS             8593..9090
                     /gene="coaD"
                     /locus_tag="JA154_04010"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089073.1"
                     /note="Catalyzes the conversion of ATP and pantetheine
                     4'-phosphate to diphosphate and 3'-dephospho-coA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAT7995024.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            9094..9618
                     /locus_tag="JA154_04015"
     CDS             9094..9618
                     /locus_tag="JA154_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995025.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            9962..11881
                     /locus_tag="JA154_04020"
     CDS             9962..11881
                     /locus_tag="JA154_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT7995026.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSIIELINKQN"
     gene            11894..12181
                     /locus_tag="JA154_04025"
     CDS             11894..12181
                     /locus_tag="JA154_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAT7995027.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            complement(12556..12690)
                     /locus_tag="JA154_04030"
     CDS             complement(12556..12690)
                     /locus_tag="JA154_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995028.1"
                     /translation="MSYPSALSVKVYPLPLPIACSVLPSRGIVVEVSPIRSLPADCPF
                     "
     gene            12964..13062
                     /locus_tag="JA154_04035"
                     /pseudo
     CDS             12964..13062
                     /locus_tag="JA154_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            13064..14182
                     /locus_tag="JA154_04040"
     CDS             13064..14182
                     /locus_tag="JA154_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT7995029.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNVLKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPQGRILNAAISKEPSGR
                     YYVSLCCTDVDIEAFENTNNQIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNSKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            14333..15550
                     /locus_tag="JA154_04045"
     CDS             14333..15550
                     /locus_tag="JA154_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAT7995030.1"
                     /translation="MLIGLIAAFGLVLQKKKASDVIKGSLLAAFGIIIMETGTGMLVS
                     SIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAIHLMIARFTPIKTIF
                     LTGHMLYWFPFIFVASGVEAGLNGGVLITFAAVLSALYWSIMPWLLRKYVFDVIGDDS
                     FTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREISISGGMAVFLMFIVV
                     GIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQIMPAFQGISEKVVPN
                     ALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILIYNYFNVFNLLLIPLVITSFFECG
                     AAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNWILIFGGNDLSLFGI
                     ISRFIGSLFGGIL"
     gene            15553..16293
                     /locus_tag="JA154_04050"
     CDS             15553..16293
                     /locus_tag="JA154_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995031.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            16306..16416
                     /locus_tag="JA154_04055"
     CDS             16306..16416
                     /locus_tag="JA154_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995032.1"
                     /translation="MLMNFEVYQQRILIIYNKCYHLKAVGKNLQLFIIVD"
     gene            16649..17926
                     /locus_tag="JA154_04060"
     CDS             16649..17926
                     /locus_tag="JA154_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAT7995033.1"
                     /translation="MGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSKKDGEKEKLVLW
                     MPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISAGEGPDVGYMYA
                     EMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAANPGVLYYNKDI
                     LEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFGNLNWNWYPYLW
                     QAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEMIESVFGPGKAA
                     FTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSSAKNKELAYKLI
                     QHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPLVVGPHGVEIYE
                     YLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            18024..18941
                     /locus_tag="JA154_04065"
     CDS             18024..18941
                     /locus_tag="JA154_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAT7995034.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            18957..19766
                     /locus_tag="JA154_04070"
     CDS             18957..19766
                     /locus_tag="JA154_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAT7995035.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDTMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            19824..21572
                     /locus_tag="JA154_04075"
     CDS             19824..21572
                     /locus_tag="JA154_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995036.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVSTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            21834..22856
                     /locus_tag="JA154_04080"
     CDS             21834..22856
                     /locus_tag="JA154_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HAT7995037.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            22861..25167
                     /locus_tag="JA154_04085"
     CDS             22861..25167
                     /locus_tag="JA154_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAT7995038.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKKILDSKPSEKKEKSIDEFEPMEYI"
     gene            25178..26254
                     /locus_tag="JA154_04090"
     CDS             25178..26254
                     /locus_tag="JA154_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995039.1"
                     /translation="MKTLKVFFCSTKYLILVFIFFINTTFISSATSDKILDLSFKKIE
                     TDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNFYIEIQNKNKSSQR
                     INLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEFEGKIYVNFNSLIN
                     EESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKLRFLNNIKIIGDEE
                     VQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQNGKLTLSNKAKPGQIILQV
                     NVDDKFKIGKKITLTESWSINKKDKDGVPYTLVSPEQSPTVQDMKKINFMNNIITFVR
                     ILFVSLVIICFGIYLYWKKCSKTK"
     gene            26272..27690
                     /locus_tag="JA154_04095"
     CDS             26272..27690
                     /locus_tag="JA154_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HAT7995040.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPNIILPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            27705..28913
                     /locus_tag="JA154_04100"
     CDS             27705..28913
                     /locus_tag="JA154_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995041.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(28987..30375)
                     /locus_tag="JA154_04105"
     CDS             complement(28987..30375)
                     /locus_tag="JA154_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:dicarboxylate symporter"
                     /protein_id="HAT7995042.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            30716..32050
                     /locus_tag="JA154_04110"
     CDS             30716..32050
                     /locus_tag="JA154_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089054.1"
                     /note="CoADR; specific for coenzyme A disulfide; requires
                     NADH; involved in protecting cells against reactive oxygen
                     species by recycling coenzyme A disulfide which can reduce
                     hydrogen peroxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAT7995043.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(32192..33109)
                     /locus_tag="JA154_04115"
     CDS             complement(32192..33109)
                     /locus_tag="JA154_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAT7995044.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            33317..34201
                     /locus_tag="JA154_04120"
     CDS             33317..34201
                     /locus_tag="JA154_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995045.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
BASE COUNT        13332 a         3739 c         5938 g        11225 t
ORIGIN      
        1 ttatattgag agtaaatata ctataattat ttcaaaggca gacagagttt atctatataa
       61 tcattttata actaaaagtt ctcttttgga taacgtttcc aatagggtag atactaaaat
      121 tatagagtat gttgaagaaa ttttagaagt aatcaacaat caatatactt ttgaccttag
      181 aaatgatagt gtattatttg atgatttagt actgcacttt aaatcaattc tcaattctaa
      241 atcttataat ttaaataagg tgaatcccct tataaataca ataaagagta attatccttt
      301 agcatttgaa ataactttaa atgctataga aaaagttttt aaaaatagta tttactcttt
      361 aacagaagat gaaattggat atgtatccct gcatataggt gctggaatag agagattttt
      421 tcaaagtaat attaagtgta aaaacgtagt attagtatgt ggttcagggt atggttcaag
      481 taggttatta gaggttcaat taaacaaagt ttttcatgat aaaataaata tacttcaatg
      541 tctatcattt aatcaatttt tagctagtga attgtcagat gtagatataa taatatcgac
      601 aattccttta aatcatgata gtattcctat tgtattagtg gatttaaaac tattaaaaaa
      661 agatatagag aatatatcta agtcaatcac taataattcc catatttata gtaatctttt
      721 ggacaatttc tttgataaaa atttatttat tgttaatcct aaaatcaaag ataaagatga
      781 gcttataaag ttgatgtgca acaaattaac tcaaagtgaa attgtatttc catcatttgc
      841 tgaatctgta ttttacagag aaagtttatc ttctaccaat atagatgatt ttttagctat
      901 acctcatcct atggaattaa gctctataag aactaagatt tgtatttcca tattggatga
      961 accaatatat tggagtgaag attctactgt taaattaatc atgatgttgg ctataaataa
     1021 agatgattat ataaaaataa actccattta tgatatcctt ttaaagataa ttcatgacaa
     1081 tgatattaga gatagtatat ctaattgtaa tgattttgat aattttcttt ccataatcaa
     1141 gtctatagta tagattttta tattacataa gttttaaaaa ctttaataaa atattttttg
     1201 tttttagcat aaatagtata agtaagtttt gtataatttg ttattatttt gtattgatta
     1261 taattattat acaataaaga ttctgtctca aaatagagag agaaacctgt ttttcattga
     1321 taaaatgata aaaatatatc tgatttcata caaaaaagag tgtctcatga actaaaaagt
     1381 tcattttgag acaccacctt tattatttta tattatttat ctcttgtata tattactata
     1441 gcatccctta aaaacttagc taatccaggt tgttctctat cataatatga tgtaaaacgt
     1501 tcatcatata catacatctg tgctagagag gaatgagctt ctttagagta tgtgttccaa
     1561 gtgtaactca accatttatg atgtaatttt gcaacttcct gagccagttc actagaggca
     1621 tcaccagttt taaaagcttt tttaagttta ctaattattt cgatatttag gttttgccaa
     1681 ttttcataat cttgcttaga catattttta agttttttat ttgatgtatc tataatatct
     1741 ttaccatatt tctctctaat ttcagttcca taattttttt cattttcatc aatcatcttt
     1801 tctttaaaac cttcaaattt ttctatatct gtcatagtgt attttccctc cttcagagct
     1861 atagttttag ttacattttc tattagtaaa tcaatttgct ttcttttggc tagtaattta
     1921 ttatggtgtt cttttaacgc acttaaacta tcgaagtttg gtgaatttat aatgtttttt
     1981 atattttcta agttaattcc taattctcta taaaaaagta tttgttgaag tttattaact
     2041 tcattttgac catatatacg ataacctgaa gaatttattt taagtggttt aagaagacct
     2101 atttcatcat aatatctaag tgttctagta cttataccag ctagtttact taatttttga
     2161 actgtatatt ccataagtag tacctcctat aaattaaata tacactttta cgcaacgtaa
     2221 atgtcaatac attaaaatat aaaaatttat atcttttaat aaaaatataa aaaaatatct
     2281 ttactggata ttttacacac aactgtcaat actcactaat gaaaattaaa aaataaaatt
     2341 tatagactat ctttactaaa aaatagaggg tctatagtct aggaggaact gtatgaattt
     2401 aaaaagaagc aatgaaaaac gagaagagta cagaagaatg tattcagata aagaaagttt
     2461 tttaagtttg attcaaaatt ttacaagtgt ctcaatagca aaagaattaa ctcttaaaaa
     2521 tatagagtta gaaacatctt ttatatgtga atataaagga aaagaagttg atataattta
     2581 taaggtattt tctaaaagtg gcaaagtatc acattatata gtgttagaat ttcaaacaga
     2641 aatggatact gaaattgtcc caagattgaa atcgtataga gaacaaattt ggaaaagttt
     2701 tattatgaaa aaaagccttg aagaaataga agataaaaat tttaaacttc ctaaagttat
     2761 accagttgtt ttatatagtg gacctgaaag gttaagtaga aaaaaaggaa ttttggatat
     2821 tgtagaatct atatcggagt tcagtaataa ttctagtatg aaaaattaaa attacataat
     2881 gtgtttattg gtctaaaatg gaaaactgtc gcataaactt gaatatgaaa atatttatag
     2941 atacaggttc tatgtggagg tgttccatga gaaataacaa gtctaaggta ttgctaggaa
     3001 tactttgtct agcaacagga atggctgtaa ttctgtcaat gttgcttcct ggctggattt
     3061 ggtcagcact taccgcatta atacttattg gatgtggagc attattattt ttttgttaaa
     3121 atagttttaa ctataaagtg tggggtgagt attgatgaaa gtagtaacaa taaaattacc
     3181 aaaatggatt tcaagtataa tcctaaaatt tatgagaaag agtaaaaatg attaaattaa
     3241 aaagcctact tacggaagta ggcttttaaa cgtggttact aagctctttc aattttacca
     3301 gaacgtaaac atctagtaca aacttttact ctcttaggag ctccatctat aatcgctctt
     3361 acgcttctta agttagctga ccatgttctt ttattatgtt tatttgagtg tgatacttgg
     3421 tttccagaaa ccttaccttt accacatacg ctacatactt ttgccatcta cgcacacctc
     3481 cttaaaatga taactattta aagactaaaa acatatttat attatcataa ataacaaaaa
     3541 tttgcaatag aaatatatat ttgccttaca catattgaag aatttcttat tatcatataa
     3601 aattatatat ataattttat ataaagtaaa tatatttgga aaataattat tatttttctt
     3661 tgaatatata tactatagaa gaaattttat ccaaaaactt ttctaggttt aaattatacc
     3721 atatttatag tatgatatga ttataatttt atataataaa taagtaaaaa tagtaaaaaa
     3781 taaattaaga aatgttttta ataaaaataa gcttaagggg gtcaaccatg agtgcaaagg
     3841 taacgaatca atatggaaat atagaaatag ataatcaagt aatagctcaa gtcgtatata
     3901 gagctgcaat ggaaagttat ggcttagtag gtttagcata taagtctaaa ggaatagtag
     3961 agctattaaa aggcgagaac gcaactaaag gtgtaagagt tgaggaagta gaagaagatg
     4021 ccatagcaat cgaactttat gttataatac aatatggtac aaatatatct actgttgcca
     4081 ataatataat agatagagta aaatatgtag ttgaaaaaat gactggcgta agagttacta
     4141 gaattgatat taatgtacaa ggaataagag ttaagtaatt tcataggagg aaacaaatga
     4201 ttcaatatat agatggtaaa aggttaaggg agatgtttat ttcaggtgca aacaatctcc
     4261 aaaacaataa agagttagtt gacaagttaa atgtattccc tgtaccagat ggagatacag
     4321 gaactaatat gtcattaaca atatcttatg ccttaaaaga attagcaaaa gttgaaaatg
     4381 ataatatatc agatatcgga aaagcattat ctaaaggttc tttaatggga gctagaggaa
     4441 actcaggagt tatactttca caaataatca gaggtatagc taaatctata gaaggaaaaa
     4501 gtaaattatc aacagaagat ttagcaaaag cttttaaaaa tggttcagac acagcatata
     4561 aagctgtaat aaaaccaatt gaaggaacta tacttactgt agttagagaa agtggagagt
     4621 ttgcaataaa aactgcgaaa aaagagaaag atgtagtaaa gtttttaagt atgcttgtga
     4681 aggaatcaaa ttcttcatta gaaagaactc cagaccttct aaagaatcta aaagaagctg
     4741 gtgtggttga ctctggtgga aagggtcttg tacttatata tgaaggaatg ttagcttcta
     4801 taaaaggcga taacatagaa ataaaaaatg ttgacctaga tactaatatt tcaacaagta
     4861 tggattttgc aaagagtact actagcacag atgatataaa atattgctat tgtacagaat
     4921 ttattttaga aagttctaag gtagaagata ctaaaataag agacataatg atggcttatg
     4981 gtgatagcct agcagtagtt ggagatgatg gagtcataaa ggtacatgta cacactaatg
     5041 acccaggaaa tgtacttcaa gaagctctaa agtacggtca attattaact ataaaaatag
     5101 aaaatatgaa gttacaacat gaaaatacat tactggatgt agaagaaaag aaagaaaatg
     5161 atagtgaacc tttagaagaa gaaaaagagt ttgggtttat agctacttct atgggtgaag
     5221 gtttggcaaa tatatttaaa gatttcggag ttgaccatat aatagaaggt ggtcaaacta
     5281 tgaatccaag tactgaagat tttatgaatg ctataaagca tataaatgct aaaaatatat
     5341 ttattttccc aaataacagc aatataataa tggctgctaa tcaggcaaaa gaattgagtg
     5401 ataaaaatat aatagttata cctacaaaaa atacgcctca aggatttgct gcattagtaa
     5461 cttttaatgg agaattaagc gaagatgaaa ataaagaagc tatgatgaat gctttaaact
     5521 cagttaaatc aggtcaagta acttttgctg ttagggatac agtgatgaac gaaattgatg
     5581 ttaaagaagg taatataatt ggaattgcag aagggaattt attatcagca ggagattatg
     5641 tggatgaagt aacatcaaac cttattgaaa agttggttga tgaagatact gctataataa
     5701 cattattctt tggtgaagat gttacagaat ctcaagcaaa tgaactacgt acttctttag
     5761 aagaaaagtt tgaagatgta gatgtagagt tatattatgg aggacaacct ctttattatt
     5821 acttaatttc agttgaataa tgattataag aacctgtatg accaagtagc ttttataaaa
     5881 ttggttgtac aggatttttg tgtagtaggt gatatatttg ttggatttat ataaagatgt
     5941 tcaatatgtt aagggaatag gacctaaaaa agcagataaa ttaaataaac tgggtatatt
     6001 tacactaaaa gacctattgt attattttcc aagacaattt gaagatagaa ataatctaaa
     6061 gaaaatagca caattagaag atggtgagaa agttacgata aaagcagtaa tatcaagtat
     6121 aaatacattt agcccaaaag aaggtatgac tttaacaaag attgatgtaa aagatgagac
     6181 aggctcggct aaattagtgt tttttaataa gagttatata aaaaatactt ttcgtccagg
     6241 agattcaata ttagtttttg ggaaggttaa gaaaaaattt aataacttag aacttacatc
     6301 atgtgaatta gaatatctta ctaattcacc taagaataca tgtagattta tgcctgtgta
     6361 ccagcttaca tacggtgtta caaataaaga aattatgagt ataattagaa cagtacttga
     6421 agataaagaa ttaattatac aagaatatat gccacaaaga attatagaaa aatataggct
     6481 atgtagtata gattttgcag ttagaaacat acattcacca agtagtaagg aatcattaaa
     6541 aatagcttta tatagaattg tatttgaaga attacttata ctacaactag ggttgtttgt
     6601 atttaaaagt ggcagaaata aagaagatgg aataaagttt gaaacaagta aagacttaaa
     6661 aaaaataata agtgctttac catttaaact gactaaagct caaaacaggg cattagatga
     6721 aattatacaa gatatgaatt tagagaaaat aatgaataga ttggtacaag gtgatgtagg
     6781 gagtggtaaa acagtagtag ctctcttagc acttgcaaat tgtgttttaa atggttatca
     6841 gggcgctttg atggcaccta cagagatatt agcagggcaa cattatattt cacttactga
     6901 gtctctaaaa gattttggaa taaatgtagg tctattaata ggtagtctta caaaaaaaca
     6961 aaaggataca gtattggaac aaataaagaa taatgagatt gatatattaa taggtacaca
     7021 tgcactaatt gaagataagg ttgaatttaa taatattggt ttagttataa cagatgaaca
     7081 acatagattt ggtgttatgc aaaggagcaa actttcattg aaaggagcaa atcctgatat
     7141 attagtaatg acagcaacac caataccaag aactttagca cttatactct atggagattt
     7201 agatatttct attatagatg aacttcctcc aggaagacag ccaatagaaa caattgctat
     7261 tgaaaaaagt aagcgagata gagcatataa taaccttgta agaagagagg ttgaatcagg
     7321 aagacaagtg tacattgtat gtcccttggt agaagaaagt gaagccatag aagccaaatc
     7381 agcagttgaa cttgttgagg agttaagagc ggaatatttt cacgacttaa ggcttgggtt
     7441 gcttcatggt aaaatgaagt caagtgaaaa agatgaagta atgagacttt ttaaagataa
     7501 agaaatagat attttagttt caacaacagt aatagaagtt ggtgtcaatg tgccaaatgc
     7561 aacattaatg ataatagaaa atgcagaaag atttggactt gcacaattac atcagcttag
     7621 agggcgagtt ggaagaggta gtcataaatc gtattgtgta ctaatttatg attctaaaac
     7681 agatgtatgt agacaaagaa tggctataat ggaagagacc aatgatggtt ttaagatttc
     7741 agagaaggat ttagaaataa gaggacctgg ggaatttttt gggacaaggc agcatggtct
     7801 tccagaactt aaggtagcaa acttatttaa gcatataaaa atactgaagt tagcacagca
     7861 agaagctcga tatatattgg gtgaagataa taatctacaa ttaaaagaaa acatggcact
     7921 caaaaaagaa attatagata aatttaaaga tacattgaaa gaaatttcat taaattaatt
     7981 ttaaattgtg ttaaaatatt actaatgtaa taatgtgctg aagggagatt gtcatattga
     8041 gagtaatttc aggaaaagcg agaggattaa aactaaatac tccaaaaaat gaagatgtaa
     8101 ggccaactac agatagagta aaagagtctc tatttaatat gataaattca tatataatgg
     8161 aaagtgaagt tctggattta tttgctggga ctggttcttt aggaatagag tgcttatcta
     8221 ggggtgccaa gtcatgtact tttgtagata tcagtaagga aagcatagat attgttaagt
     8281 ccaatataaa aaaggcaaga gttgaaagtg aaagtattat tttaaacctt gattttaaga
     8341 ctgcaataga taaacttaaa ttacaaaata gtaaatttga cataattttt atggaccctc
     8401 cgtattataa aaatatgttt atagaagcta ttgaaaaaat tgataattct aatctattga
     8461 acgaagatgg aataatagtt gtagaacatg atacgaatga tttatttcca gataagatat
     8521 gtaaattgga gaaaactaga gataagaaat atggaaatac tacattaact ttttataaga
     8581 tggaggcata atatggaaaa taaacctaga aaagctatat ttgcaggaag ttttgaccca
     8641 attactaatg gacatttaga tattatttgc agggcttcga agttatttga cgaattacaa
     8701 ataggagtat taaataatcc taataaaaag ggtctattta gctttgatga aagagtaaaa
     8761 cttatagaaa aaagtacaag ccatttaaat aatataaaag ttgtgacttt tgatggttta
     8821 ttaataaatt actgtcagga aaatgggatt ggagcattag ttagaggtgt gagaagtggt
     8881 gctgatgttg attatgaact tcagatggcg catatgaata gagagcttaa tccagatata
     8941 gagactataa ttttaccaag ttgtacaaaa tattcattta taagttcatc tttaataaaa
     9001 gaggttttgc tctttgatgc tgatataaaa aatttggttc caaagattgt tttggaagaa
     9061 ttaaagaaaa aaactagtgg gggtaattga tatatgaaga tagatttaga aatgattgaa
     9121 ctatttgagc agttagagga gatatttaga agtgcttcta caatcccttt ctcacataaa
     9181 tgcacagtgg ataaagatga ggttttagca ataattaatg atataagaac tttaatacca
     9241 gaagaagtaa cacaggcagt atggattaat aaagaaagaa ataaaataat aagtcaagct
     9301 aagcaagatg ctacaaatat agttgaacaa gcgaaaaaag aagctgagag aatacaacaa
     9361 gaatatcaag ataatataga ggaattaaag aaaaattctg aagatgtagt aaaggcatat
     9421 gtagaatcca gtgaacctgt tgttcaagca gaccaaagag caaaagatat agttgataga
     9481 gctgagagaa tagccaatga gataagaatt gggtcaattg aatatgcaga ggatgtgctt
     9541 tctagtgttg agtataattt aagagaaata ttagaagaag taaaaagaga taaagctgaa
     9601 cttagaccta aaaaataaat tttaaataaa taaaaatgga gctatcacac aaaaaattcc
     9661 aatttatgtg tgatagctct cttttaatat agcaattttt tgttatataa ttgtgctttt
     9721 tagattactt atatcttatt ttgatattat aatgtagttt aatcttttat ttgttgaaat
     9781 caacttattt atttgtataa ataatatagg ttattttcaa atttttttaa taattttacc
     9841 actgtcttct tttagattag agttagactg tgcaaaatta taaaataata ctaaatcatg
     9901 tgcaattatt tggtatggca ctacatgtag aaaatataga taataaaatt aaggaggtag
     9961 tatgaaggaa agggctaaaa aaatattaga ttttattatt aaaaataaca atgtaacatc
    10021 acaagaacta caagaaaagt ttaatgtttc aaagagaact atttattacg atattctagc
    10081 tataaatgaa caactaggaa aaagtggtaa tataaaaaat gtaaaacaca aatttatatt
    10141 tgaaggtaat ttatgtgatg caaggaaaat aatttcaact gaagaagata aatttttaga
    10201 tagtgattac agaaaaactt ttatattaaa caagatatta cttggcgaaa aaatatcaat
    10261 agaaaaacta acaaatgaaa tgttactttc aaaaaacaca gtagtacaaa caataactga
    10321 tgtaaaaaaa taccttcaaa caatggggtt aagattagag tataagggaa agtacaaaat
    10381 tataggtgat gaatacgtta ttagagagct gtttctgata attgttcaag aaaatgtact
    10441 ggaaattaat tctataagtg aggaagtaag tagtttcgat acaaaaggac atataaaact
    10501 tactgattat tctttattaa atctaactaa gtttgtggaa tttctaaata aaaggataag
    10561 agatgggaaa acattatact catataaata tctaaatgaa gctaaaaaaa ttagttactt
    10621 tagtaattgt aaagaacttt tatgtgaaga agctaatgaa aatgaacaag cctatatatg
    10681 cacttacata agttcacttc ccagcttaaa tagtgaagtt aaggaagatg ttgttgaaga
    10741 atatgtagat aaacttatag ataagtttga agtaaataca gcaataaaat tagaaagtaa
    10801 gcatgagttt aagaaaaata ttttaaggca cctccactct tcttacaaca gaatacgatt
    10861 taagtttcct attagaaatc caatgttaga tgagactaag tataaacatg agtcacttta
    10921 taaaattata aaatcaatta tagagaatga agaagagttt cctgtatttg aggggataag
    10981 agaagaagaa ataggattta tagcagcgta ttttggagga tatttaagag ggtctaggga
    11041 taatggtcta agaagaaata aagtcttact agtttgtcct aatggtctta tggtatcaaa
    11101 gagtttggaa atacaactat ataaatacat accaaccata gaaatagtag gtatagtatc
    11161 cattaagcaa ctaaaagaag taaatgtata ctacgattac ataattacaa ctatagatat
    11221 acaaaatgta aacaacgtaa ttgttgtaaa tcctctttta acatcatcag atgttcaatt
    11281 acttatgaac aaactaataa gtgtaaaaga aaacgagaag tatttcaatc ttgaattaat
    11341 aatacaagct ataagaaaaa atggagttat caataatgaa gaagcactaa aagctgattt
    11401 attaaacata atacacaaga tagatgaagg agagatgtat caaccaatgt taaaggaatt
    11461 aattaatgca gaaagggtca atataataaa aaatgttaga gattggaaag aagcaataaa
    11521 aatagcctca aagccattgt tagaagataa ctctatagaa gagttatata tagaaaatat
    11581 gataaagtca gttgagaaat atggaccata tattgtttta gctgatagat ttgctctacc
    11641 acatgcaagt tctaaagaag gagtaaacaa gcttgctatg tctttactaa tagtagaaga
    11701 tgaagttgat ttattaggaa aaccagtaaa tatatttatg gtattagctg ctgtagataa
    11761 cactactcac attagagcat tagcatcact atcagagatg atgtacgagg aagaaaatgt
    11821 aaaactaata attaatggtg ataaaagttc aataatagaa cttataaata agcaaaatta
    11881 gggggaatga aaaatgaaga tattagcagt atgtggtttt ggtgtaggtt catcaatggt
    11941 attaaaaatg acaatccaaa aggctctaaa gcagttaggt atagaatgtg aagtagaaaa
    12001 tacagatata aattcagcta gaggtgctga ttgtgactgt atattcacaa gtcaagaatt
    12061 gttaaatgaa ttaaaaggaa cttgtaatgt agatgtatat ccagttaaaa agtacatgga
    12121 tttagcagaa gtcacagaag ttgttcaaac ttttttaagt gataaaaatg ataagaaata
    12181 gtataagttt taaaaagtaa atttaagtta atcagaataa aataaaaact tactaagttc
    12241 aaaaatagta agaaagagag gagggattta gtggtagaaa acttaaacag ttgacctatc
    12301 actaaaaaat ataatgcttg attaaaaaat agctacgcac actcgtgacc ttagtcatga
    12361 gttagtagct aaaatagtca gcatataggg aaacctatat gtagagatag gatagaacct
    12421 atccaatact acttgaattg ctggaaaccc ctaaagctaa ccaaactaaa acataaggat
    12481 gaaataaacc taagtgtgaa agttacgaaa gtagaaaaaa ttggttagat ggtataaggt
    12541 taaaccctaa gtactttaaa atgggcaatc agcaggtaag ctccgaatag gagaaacttc
    12601 aacgactatt cctcttgagg gaagtacact acaagctatt ggtagtggaa gtgggtagac
    12661 cttaacggat aatgccgaag gataagatat agtctgtgct tacgtgaaag tgtaagaagt
    12721 tcataagaga actgcatagg tagtagcgaa cttatgtgaa cgacaacctc aaaaatgata
    12781 aaagttaaga acttacggtt cttatatatg ttatttaaaa ataaatataa aaaaccttca
    12841 aaatacttgt tttaagtaat tgcatatttt aatattatga taagtgatga aaaatggaag
    12901 ttactcatgg tagaggatat gcatatagta tgtcaaatat agacatccat tattatgggg
    12961 agacatcttt ggaatcctag ttatttcgtt gctactgtat tagaaaatac agaggagcag
    13021 ataggaaaat atattaataa tcagaaaaaa caagtaaagt gaggtgatag tagtggaaaa
    13081 agcttataag tttagaatgt atccaaataa aaaacagcaa gagttaatta acaaaacttt
    13141 tggatgttgt agatttgtat ataataagta tcttgctaaa agaatagaag tttataaaaa
    13201 cgataaagaa acttttactt ataaacagtg tagttctgat ttgaccaatt taaaaaaaga
    13261 attaaagtgg cttaaagaac ctgacaaatt ttcactacaa aacgtcttaa aagatttaga
    13321 taatgcatat aaaaaattct ttaaagaaaa agcaggattt cctaaattta aatcaaagaa
    13381 aattaataga ttttcatata aaactaactt tacaaatgga aacattatgt attgtggtca
    13441 acatataaaa ctacctaaac ttggtatggt taagataagg gataaacaag tacctcaagg
    13501 tagaatactt aatgctgcta tatctaaaga accaagtggt agatattatg tatcattatg
    13561 ctgtactgat gtagatattg aagcatttga aaatactaat aatcagatag gtttagattt
    13621 aggaattaaa gaattttgta tctcaagttg tggagaattt atagaaaatc ctaagtatct
    13681 taagaagtca ttaaacaagc ttgctaaatt acaaagagaa ttatcgagaa aaacaatcgg
    13741 tagtttaaat agaaataagg caagattaaa agttgcaaga cttcaagaac atatagctaa
    13801 tcaaagaaat gattttctac aaaaactatc tactaaatta ataaaagaga atgatattat
    13861 ttgcatagaa gatttacaag taaagaatat gattagaaat agtaaactat ctcgcttaat
    13921 ttcagatgta tcatggtcag aatttattcg tcaattagaa tacaaggcta attggtatgg
    13981 tagacaaatt gtaaaggtag gtaaattctt tgctagttca caaatatgta ataaatgtgg
    14041 gtataaaaac gaggaagtta aggacttaaa tataagagaa tggatttgtc ctagttgtaa
    14101 tgaaactcac gatagagata taaatgcaag tataaatata ttaaaagaag gattaagact
    14161 aataacgatt caaaataaat aacatatgta agaaccgtag gaactacggg gatagcctgt
    14221 ggagaattag taagacatat tttagtatgc aaaattctat gaagcaggaa ccctgtgact
    14281 ttagtcatgg gaggttcagt ttatttcaaa taatatttta cgtaatccat ctatgttaat
    14341 tggattaata gcagcatttg gtcttgtact acagaagaag aaagcatctg atgttataaa
    14401 ggggtcacta cttgcagctt tcggaattat aattatggag actggaacag gtatgttagt
    14461 tagttcaata gcaccaatta atggagcttt ccaaagtata agtggtggtg cagtaacaaa
    14521 aggtttaagt gatgttactt ttactgcaag ttatggtgga attataggtt tagcaatgtt
    14581 tgcgggttta gctattcacc taatgatagc aagatttact cctattaaga caatcttctt
    14641 aacaggtcac atgctttatt ggttcccatt catatttgta gcttcaggag ttgaagctgg
    14701 attaaatggt ggagtattaa taacttttgc agcagtatta tcagcactct attggtcaat
    14761 aatgccttgg ttattaagaa aatatgtgtt tgatgttata ggagatgatt cttttacatt
    14821 aggtcatcca tctggttgtt tagcacttat atcaggattt gttgctaaat tagtaggaaa
    14881 taaagaaaaa tctacagaag atataaacat acctcaaagt ttatcatttt ttagagaaat
    14941 atctataagt ggtggaatgg cagtgttttt aatgtttata gttgttggga tagtaatacc
    15001 aggtgcgttt aaagatagta atgagtttat tggaacgtat gcaataaagc aaggtctttc
    15061 ttttggagca ggtcttataa taatgcttca tggggtaaga atgctaataa atcaaataat
    15121 gccagcattt caaggtatat ctgaaaaagt tgtgccaaat gcactacctg cttttgactg
    15181 tccaatatta tttaattata aaccaaatgc tgtattaata ggattcttag ttgctatgct
    15241 aacatctaca gctatgattt taatatataa ctactttaat gtatttaatt tgctacttat
    15301 accacttgtt ataacttcat tctttgagtg tggagcagca gcagtaatag gagaatcaca
    15361 aggtggtata cgtggagcta taataggtac agctatagct gcaatattta tggtagtttt
    15421 agtaggaata tctgctgtaa tttactcagg aactattcaa aattggatac taatatttgg
    15481 aggtaatgac ctttcattat ttggtataat cagtaggttt ataggttcac tatttggagg
    15541 aatattatag atatgaaatt taaatacata gatgagataa gaagattaat tgatataata
    15601 gaaaacgaag aaaaagtcaa tatggaaaaa gcagtacaaa tctttgttga agcaatagaa
    15661 agtaaaaatg ctatattttc atttggagct tcacatgcag gaatattaac agaagaatta
    15721 ttctatagag caggtggctg tgttcttata aatccagtat ttgcaaaatc tctaatgtta
    15781 gatacagcgc ctataactca cacaagtaag atggaaagat tagaaggata tggtacagct
    15841 ctagctgata aaactccaat taaaaaagga gatgtagtta tagttcattc agtatctggt
    15901 agaaatccag taagtataga gtttgctatg aaagctaagg aaaataaggc aatagtaata
    15961 tgtataacta atttaagtta ctcaaaaagt gtatcttcaa gacattcttc aggaaaaaaa
    16021 ctttatgaag tatgcgattt agtgatagat aatcatggtc aaaagggaga tgcctgtgta
    16081 gagatagatg gattaaacca aaaagttagt ccaacttcta ctgtaatagc aactactgtg
    16141 atgaactcta ttatagctca agctactcag gaattagtta ataaagggct aaagaatcct
    16201 ccgattttct atagtgccaa tattgatggt ggagatgagt taaataagaa gatttttgat
    16261 gaatataaaa gtgtaattca ttatgaatat taaggttaaa aaaatatgtt aatgaacttt
    16321 gaagtttatc aacaaagaat attaataata tataataaat gctatcattt aaaagctgta
    16381 ggtaaaaatc tacagctttt tattattgta gattaaaaag atgcgtaatt aaattttaaa
    16441 ttggagaaaa taaattggaa ggtatcaatt ataaatatta gaaacgttgt catagaaaaa
    16501 aactatatta aaaaaaagta catagaaaaa actgattaga caataaatac atgctttgat
    16561 aatattttaa atggaatgaa aatgtgtaga attataatga aaaaagaaaa taaaaatgaa
    16621 aaaagaaaat aaaatggagg gaataaaagt gggtatgtta aaaaaattat gttcactagc
    16681 tatggtagca attatgacta caacacttat aacagggtgt tcaagtggaa atggaaaaga
    16741 ttcaaaaaag gatggcgaaa aagaaaaact agttctttgg atgccaccag tagaaaataa
    16801 tatgaaggaa gtttgggacc ctattttaga taaatttgag gaaaaaaata actgtgaagt
    16861 agatttacag ataattcctt gggataatta ttctgaaaaa tttgcaacag ctataagtgc
    16921 tggcgaaggt ccagatgtag gatatatgta tgcagaaatg tatccacagt ttatagaaag
    16981 tggagcagta gaagatttga ctaactatgc aactaaagag gataaagagc aatctattta
    17041 tataaaaaca tctgaaatga tgggtggaat gtatggtatg ccattccaag cagcaaatcc
    17101 aggggtatta tactataaca aggatatttt agagaaacta ggagaaaaac ctcctaaaac
    17161 atgggaagat tttaaaagaa tttgtaaaaa agcaacgcaa gatactgatg gtgatggaaa
    17221 gatagaccaa tggggattag ctcagggatg gggagctaaa acttttggta acttaaactg
    17281 gaactggtat ccttatttat ggcaagctgg tggagatata tttaatgatg atttaaaatc
    17341 ggttaaattt aatgataaga gtggtttaga agcagctaat ttcttaaaag aattacaagc
    17401 atatgtgcct gaagattctt tatctaaaga tagtaatgaa atgatagaaa gcgtatttgg
    17461 accagggaaa gcagcattta caataatgtt atcttctgct gcaacaagtg tatttgatga
    17521 atcattcccg gatttaaatt ggggatttgt aactggacta gaagataaga aagcagcaac
    17581 ttttggagca gtagaccatt tatctttaat gtcatctgct aaaaataaag aacttgcata
    17641 caagttaata caacatatgt taagtgttga atcaatgaca gaatttcata aagcaatacc
    17701 aagagcacca ataacaaaag gtgaaccata tcaaggtgat gaaagattca aagaaatggt
    17761 ggaaaatgac aaaaatgtat atagaccatt agtagtagga cctcatggag ttgaaatata
    17821 tgaatattta tggaaagaac ttcaaactat gatttcagga gataaaactc caaaacaagc
    17881 gttagatgat gctgcaaaat attcaaatga tttattagca caataaaagt ttaaagctgt
    17941 aatatgttgt aaaggacttt acagattaaa attagcattg tatttataaa tacaatgcta
    18001 atatttatat tggagaggat aaaatggaaa acttagttag agagtttgaa gaaaattcaa
    18061 aaagaaaaaa aacaaaatta aatattaaac catatatgta tatattgcca ttgggaataa
    18121 ttttagtggc gttttatgtg ataccaataa taatgtcaat atactttagt tttacaaagt
    18181 ataatattat tagtccagct acttttatag gtttagaaaa ttataaaaaa ttatttacag
    18241 atgaaacatt aaaagtatct attataaata ctattaaatt tactgttgtt gtagtacctt
    18301 gccaaactat attatcattg attttggctg tttggattac aggaaaagga aatagtaaaa
    18361 tagcttcatt tgctaaaggt gctatattta taccagtatt atcatctatg gttttgatag
    18421 gtatggtatg gagggcttta ttgaatggag aaggttctat tatatatcag gtattaggtg
    18481 cctttggaat agaatcatct aaacttttag gagatagtaa gacagcatta cctacactta
    18541 tgtttatatc tatgtggaaa aatatagggt attttatggt aatttatatt tcagctataa
    18601 tgaatttacc aaaacactgc tatgaagtag cgaaggttga tggggctaca aaacttcaag
    18661 aatttataaa aataactgta cctttattga aaccaactac aataatggtt gtatttttag
    18721 ggtcgatatg gtcattacaa gtatttgact taatttatac tgtgacaggt ggaggtccag
    18781 gtatatctac tatgagtata gttatgcatg catttaactt aaactttaaa aactttaatt
    18841 ctggatatgc aatgactgta gcaaacgtat tatttttgtt aatagcagta gtttcgattt
    18901 tacaaaataa gcttgtaaga cgagacaatt cagattttta gaaaggtagg tataatatga
    18961 aaacagcaag aagattatta agtgatggtt tgcttttatt tatagcgtgt gcaagtttgg
    19021 taccttttat atacatgttg ataatatcat tgaagataac atataactca tatagtttgg
    19081 atatatcttt ttcaacagta acccttcaaa actatataga tatttttact aaaaaaggat
    19141 ttgcacaata tttttttaat acagcaatag tatctttttc aggtgtatta ttaaatttag
    19201 tatttagtac attagcagga tattcttttg cgaagatgga ttttaaaggt agtgataagt
    19261 tatttttatt tatgataatg actttaatta taccttccca agttacaatg ataccactat
    19321 atataataat gaaacactta ggatggataa atagttatct tgcacttatt atgccaatac
    19381 caactgcatt tggcgtattt ataatgagac aagctatttt aggagtacca aaagagttat
    19441 tagaatcagc aaagatagat ggttgttcgg attttagaat tttaatacaa atagtgttgc
    19501 ctttaattaa accagcatta attacactag caatatttac ttttatggga gcatggaacg
    19561 agtttatgtg gcctcttata gctacaacta aagatacaat gagaacatta acagttggat
    19621 tatctactct taagacattc cagataacta attatggaca gatgatggca ggggctacaa
    19681 taacattttt acctccattt attttctatt tgattttaca gagtaaattt gttgaaggtg
    19741 tgtctttgtc tggtataaaa ggttaaatag tctaatttaa taatatataa agaaacttat
    19801 ataaatatgg aggaatttaa actatggaaa tttcatatgt actgaaagat tgcacattca
    19861 aacacaataa atactcagtt gtagaagctg aaacttggaa agataaggat ttggatattg
    19921 tagtagctaa aggtgaaaaa tttgcatttc aaattatgtt aaaagcaaca gaagaattta
    19981 attgtacaat tgataaaagt agtgctatat catggaaagg cctgggaaat agagtaaggt
    20041 tagcattaaa tatgcctaat agtttagaaa ataattttag tataaatata ttaggatatg
    20101 ttcaagatga tacaaaggca tttgtgaatg atgctatttt aagggataaa gatgtgttag
    20161 tagagcaata tttgcctcaa acattttgga ttgaaggtca agtaccagaa gattttgaag
    20221 aaaataacct tgatataggt attgatatat ttaaaagctt tggatatgaa gacgaagaaa
    20281 aagtttgtac aattgatgtt cctgtaaaag taagaaatgt tgtgctaaag cctttagatg
    20341 aaagcaagtt ctttttagat ttatggcaac atccaagctg tttagctaga atgtataaag
    20401 ttgagttatg gtctgatata cattttgaaa tagtcgataa ctatttaaaa gaattagcat
    20461 ctttaggaga aaaagttgca acagttatag tttcagatta tccatgggca gggcaaagtt
    20521 gttataaagt ttataaaaat ccatcaaact tatatgaata taacatggta agtgtatcaa
    20581 aaggattgga tggaaagatt aaatgtaact ttgagagtat ggataggtat ataagcatag
    20641 ctgataagta taaaatggct aaggagatag atttatttgg tctattaggt aattggtgtg
    20701 ctggtgagtt tggaaatcct gtagaaggtt ataaggaccc tataagagta agatattttg
    20761 atgaaaagga taaagtattt aaatttataa acaatacaaa tgatttaaaa gaatatatag
    20821 gattagtatt aaatcattta atagagtgtg gattatggga cagagtaagg attatagctg
    20881 atgaaccaaa taatccagaa gtagtcaaag agtgtataga atttataaat tctactgtaa
    20941 gcactcatca agttaaatat aaatcagcaa ctcacgacca aaacttttta gacagagcta
    21001 aagatgaaat tgatgatatg tcaataaatt taaaacttac tatacaaaat tataaagata
    21061 tagaaagtct taagaaaaaa atcaatgaca agggtgggat attgacttgg tttgtatgct
    21121 gttttccaga gaaaccaaat agttttttaa gttctccttt tgtagaaaat agaataatag
    21181 gatggtatac ttattacttt ggtcttgatg gatttttaag atgggactat aatttatgga
    21241 cagaagaccc ttggaaagat tcaagttaca aattccctat ttggaaagct ggagatatgt
    21301 tctttgttta tcctggaaaa gatttaaagc cagttagaag tgtcagaatg gagaatttaa
    21361 gatttggaat acaggacttt gagttattta caatgttaga aaaagaaaag ggaagagagt
    21421 atatagaagt tgagcttatg caagaattat taaataaaaa agaaaatgct gaaattaaag
    21481 gttttggtga catagaacta ggatattcac ttgataattg gggatatgat aaagttaaaa
    21541 aatatgtatt agagttgtta gacagagtat aaaaaatata agagtgtctc aaaatgaact
    21601 ttttagttca tgaggcactc tttttgtatg aaatcaatat agtttcattg tttcaacaat
    21661 gaaaaatagg attatttcta ttctaagaca gccctttagt tttttagttg tattcaatgt
    21721 tttaaaacta actataaacg ctataaagtt tataaatgac aataattata tataacatga
    21781 tgtactttaa tttgctataa tagggattga ataatattat atggaagata acattgtttg
    21841 gggggattaa tataaaaaga ataggtatta ttataatgtt ttttttaagt atatctttta
    21901 ttggatttta tcttgtaata agagataaca gtgatacagt taagatgcaa cgagggataa
    21961 atataggtaa tgcactagaa tcacctaagg attttccgtg ggatgtaaaa atgtcaaata
    22021 agttttttga tgatataaaa gatgcaggtt ttgacacagt acgtatacca gtaaggtttt
    22081 ctgattatac gtcagattct gataatttta aaattgatga agaatttttt aaaaagattg
    22141 ataaatatgt agattatgca ttggataaag acttaatagt tgtgttggat ttacaccatt
    22201 ttgaagaaat tatgaaagaa ccaagagttc ataaagaaaa gtttttaaaa atatggcaac
    22261 agatagctaa taggtatcaa aaatatgata aaaaattggt atttgaatta ttaaatgaac
    22321 ctaaagaaaa tttatattca cagttactaa acgaatacat tgaagaagca ataaagataa
    22381 ttagaaaaac taaccctaaa agaactatta tagtaggacc atataatttc tatcaaattg
    22441 attatttgaa tgaattaaat attcctaaag attctaatat agtagtttcc tttcactatt
    22501 acgagccaaa tgattttgca tttcaaggaa atatatatca taagggattt gagcacttaa
    22561 gcaatattac ttgggaagga acaaacgaac aaatggatta tttaaaaaaa aggtttgata
    22621 ctgtggaaaa ttgggcaaat aaaaataagg taaaaatctt cttgggtgaa tttggaataa
    22681 caaaagaggc accagaagct tcaagaagag catggataaa agctgtaaga gaagaagctg
    22741 aaaaacgaaa cttttcatgg gcttattggg agcttgcatc tggatttggt atatacaatc
    22801 aaattgaagg gacttgggat agagatatat taagtgcatt aatagaaaag aggtgatttt
    22861 atgaagaaaa attcaaaaaa gatacttata actttaacta ttattactaa tgtcatatat
    22921 atattgtgga gaatttttta cacagtacct aaagaagaag ggaagtttgc cttaatatgt
    22981 gccataatac ttctttttgt agagataatg ggcatgatgg aaatgtttgt gcattattat
    23041 ggtatgtcaa atattgagta ccctgaaaaa ccaatcataa gtgaagaact ttatccacat
    23101 gtagatgtat tcattgcaac atataatgaa tctgttgatt tggtgagaaa aactgtaaat
    23161 gggtgtattc atatgcagta tccagataag aaaaaggtac atatatacat atgtgatgat
    23221 ggaaatcgtg aagaaatgtg tattctagca gaaaaaatgg gtgtaaatta cataactaga
    23281 actgaaagag aaggagcaaa agctggaaat ttaaataatg caatgcaaca tacaaattcc
    23341 cctttgattg ctacatttga tgcagatatg ataccaatgc atgacttttt aattgctaca
    23401 gtaccatact ttttgaaaaa tgagcaagct aaaaaagatg gagaaaaaga ggagtatgaa
    23461 aaggttggtt ttgtgcaaac tccacagagt ttttataatc cagacttgtt tcaatttaat
    23521 ctgcactctg aaggaaggat tccaaatgag caagattatt tttacagaga cattcaattg
    23581 gctagaaata ggacaaattc tgtaatttat ggtggtagta atacagttat ttcaagggaa
    23641 gcacttgaag aagttgatgg attttatact tattctatta cagaagattt tgcaacaggt
    23701 atcttaattc aaagtaaggg gtatagatgt tatgctattc cagaagtaca tgcatcaggt
    23761 ttatctccta cagatttaaa aagtcttatt aagcaacgtg aaagatgggc acgtggatgt
    23821 atacaaactg gtagacgtct aaatattttg tttaggagag gtttaggttt ttggcagaaa
    23881 ataagttata tttcttcaat tacttattgg tatgcaagta taaaacgctt tgtatatata
    23941 atggcaccta ttttattttc tgtgtttaat gtgattgtag taaaatgtac gttacttcaa
    24001 gtattggtat tttggttgcc aatgtatatt ttaagtagtt tatctcttaa aatattttct
    24061 caaaatataa gaaatactag atggaccaat atatatgaaa ctataatgtt tcagtcttta
    24121 atgccagcag taatacttga aacgtttgca atatctaaaa ataaattttc agtaacaaat
    24181 aagagtaaat tagaagaaaa tagaatgtat aagtttttac agggaatacc ttattttatt
    24241 tacatggtct tatctatcat aggaatttta aaaatgtttg tagcaatatt taaaatgagt
    24301 tcaatgacat attcagttgt attattttgg ttgattggaa acctttttaa ccttgttatg
    24361 gctacattat ttatatcagg gagacagcag ttaagaaagt ctgagcgtta tattgctgaa
    24421 attgatttta agttaaagca aaattcttat gttctttctt caaaaacaat tgatatatca
    24481 gaaaatgggt ttgcattttt gctagaaaat ccagaatata tttccccaga agaagagttt
    24541 gaggtagagt ttagagaaaa atctggaaat gaaatgtata tagctaatat gaaagcaaag
    24601 attgttaatg ttgtggaagt aaattcaaag tggaaatatg cagcttatat tacacatatt
    24661 gaagatagtg aaatagataa ttggatgtgt attgttcacg atagaatacc tactttacca
    24721 atgacaatat ctaatcaact aggtttcttt gatgatttgc aaattaatgt aaaaaaacgt
    24781 attgaaaaaa ctagaacatt atcacgaaga agtccaagaa tcaatatgaa ttttcagatg
    24841 gatataaaaa atacaggtaa attaagaata gtgaatttta attatcaata tgtattatta
    24901 aattttgaga ataaaagtat ttatcctaaa gaaatagcat tggaaattaa tgaaaatatt
    24961 gtgttagaat gtgatttgtg tgagggtaaa attgatgaaa gaggtatctt atataaagta
    25021 aataacattg acagcataat gcaaaatttc tttttaagag atgaaatgat ggattggata
    25081 ttacaaaata agaaaattct tgattcaaag cctagtgaaa agaaggaaaa aagtatagat
    25141 gagtttgaac ccatggaata tatttaggag agatgaaatg aagactttaa aagtgttttt
    25201 ttgttctaca aaatatctta ttttggtgtt tatttttttt ataaatacta cttttataag
    25261 ttcagcaaca agtgataaaa ttttagattt atcctttaaa aagatagaga ctgacttaag
    25321 ttctaagatt acatatgagg atacaggtgt aaaaatagaa acggatagtt ctaaaagcga
    25381 taaggaacgt tatttatata tttaccaaaa tataaaagaa aattggagca tgtataataa
    25441 tttttatatt gaaattcaaa ataaaaataa aagttctcag agaataaatt taagcataca
    25501 aagtaaaaat atgcttgaat ttagattaaa agaaggtagt gaagtatttt tagaaggtaa
    25561 aaatataata tattcagata aaataaaaga aggatgcata gaagtaccag gtgaatttga
    25621 aggcaaaatt tatgtgaact ttaatagcct tataaatgag gaaagtaatg ttgtgctgga
    25681 ctcaaatatg ctttcaaaca ttgttagttg gggaattacg tttattccat ctgatgaaga
    25741 acataatatt gttattataa aaaagatatc tttattgtct gaagagaaat taagattttt
    25801 aaataatatt aaaataattg gcgatgaaga agtacaaata ccagtattgg gtcaaagtat
    25861 aagtcagtat gaggtattag gtcttaaaag tgattctaaa attaagtact ctcttatggg
    25921 aaaacaagat aatgtgagta tatctcaaaa cggtaaatta acattaagta ataaagccaa
    25981 acctggacaa attattttac aagttaatgt tgatgataag tttaaaattg gaaagaaaat
    26041 aacattaact gaatcttgga gtattaataa aaaagataag gatggtgttc catatacatt
    26101 agtatctcct gagcaatcac caacagtaca agatatgaaa aaaataaatt ttatgaataa
    26161 tatcataacg tttgttcgaa ttttatttgt atctctagta ataatatgct ttggaatata
    26221 tttatattgg aaaaaatgtt caaaaactaa gtaaagagag gaggttagat tgtgttattt
    26281 tcaagtttaa tttttttatt ttatttttta cctataactt tggttttata ttatgtattt
    26341 aggtttaacc gaacgataca gaacatgata ttgcttgctg ttagtttatt tttttatgct
    26401 tggggagagc caaaatttgt tgttatcatg atagtttcca taataatgaa ctatatattt
    26461 ggtttactgg tagatagata tagggaaagt aaaataaaag taaaagtttt tttagttttg
    26521 atgtgtgcat acaatatagg tgttttattt atatttaaat atctagcttt tgcgctaaga
    26581 aatattagtg aacttattag tactgaattg actataccta atattatttt acctatagga
    26641 atttcatttt ttacatttca aggaatgtct tatgttatag atgtatatcg tagacatgga
    26701 gaagtccaaa agaatccttt ttatgtggga ctgtatatag catttttccc acaattaata
    26761 gcaggtccta tagttagata tgagagtgtg gcagaacaaa ttctcaatag aaaagaaact
    26821 tggaataaat tttctattgg tacttgcagg ttcataacag gtctaggtaa aaaagtatta
    26881 atttcaaata acatggctat agtagcagac tatatttaca ctatgaatag tcaaggagaa
    26941 atagctgcat cacttgcatg gcttggttca atagcctata ctctgcaaat attctttgat
    27001 ttttctgctt attcagatat ggctataggc ttaggtctta tgtttggttt taaatttgaa
    27061 gagaacttta attatccata tatatcaaaa tcaattagtg agttttggag aagatggcac
    27121 atttctttgg gaatgtggtt taagagctat atatactttc cgcttggagg ttctagagta
    27181 gcaaataaag atattatgat tagaaatatg tttatagtgt ggttattcac tggaatatgg
    27241 catggagcag aatggacatt tgtaatttgg gggattttaa actttgtttt cctaattata
    27301 gagcgattta taatgtttga aaagattgaa aatcacaatt ttataaaaca tatttatgct
    27361 ttattagtag ttaattttgg atgggtattg tttaggtcac ctaatttaaa agaagcatat
    27421 aattatttta aagctatgtt tggaggcaat ggagctatat ggagtgatta tacatatatg
    27481 tttttaaaag aatactgggt tttctttata tttgcattta tttttagtgt acctattgca
    27541 aagaaaatca ataagtttgt tgttgaacaa gctaaatata gtatgatatt taacgtattt
    27601 tatccaatct cagctatttt attgtttttt atttctgtga catatttagt aactggaagc
    27661 tataatccat ttatttattt taatttttag ttgggaggaa aagtatgaat agatttttta
    27721 aagtaagatt aataactgca attatgttct taattatttt attcgctttc tctatcttta
    27781 atgtttgtta ttctggaaaa gatacgatac aggaaataaa aaattcaata gctgaaaaaa
    27841 gtagtttaaa agatacaata tcatctattg ataatacagt gaatgacaaa gtaatataca
    27901 aaaatacgtt cattgaaact tatggctatt tacaaaaatt aatgggtaaa aatgagtttt
    27961 caaaatttag tgttgtaaaa gatacttctg gaaagatgca ttatacatat tttgctaaag
    28021 ctccaaatcc agttggtgtg atagcagata gggtaagaaa attttctgat gaaatggaaa
    28081 agcaaaacac taaattggtt tacctaatga ctccagataa gtatataaaa ggagtgacaa
    28141 aatttccaaa aggaatacct tattcttata gtaatgagac agcagataat tttttaaatc
    28201 aactaaaaaa gtataatgtg gactatattg attttagaga aaatatttta aagagtggta
    28261 ttccaaaaga tgatttattt tttaaaacag accatcactg gaaagtagaa acttcttttt
    28321 gggcatttgg agaattagtt gagcaactta ataaaaaata caatatgaat ttagatgaaa
    28381 atcattatta tagagataag gacaattata attcaattgt atatcctaag tcatttcttg
    28441 gttctatggg aagaaagact ggtatcttat atggtggtat agatgatttt acattaattt
    28501 atcctaaatt taagactaat tatacttatt atacagattc taaatctcaa aaatttgaat
    28561 taaaaggaag gtttgaagag agtttaatat tatcatatcc ttttaatgca gaccttgatt
    28621 taatggatgg tcaatcagat aagtatttta catatttatt ggggaacaga ccattagtta
    28681 aaataaaaaa tatagaaaat ccagatggat taaaagtgct gtttgttaaa gattctctaa
    28741 ttgtaccaac agcagcattt ttttcctctg tttgttcatc tatagatatg atagacccta
    28801 gatattatga tggagatata ctagaatatg caaaatccca tgactatgac tttgttttta
    28861 tatctgtata tccccaaaat cttacaaaag aattttttcc gttttgtgag taaatttcta
    28921 aaaaagtttt ctgataaaat aaatggctaa ctggtaaaaa gttagccatt tattttgagt
    28981 gttattctaa gaaatatttt ttttactaaa tacttcttta tctatttttc caagtgattt
    29041 tcctacagta attgatgata ccatagcacc attaacattt atcatagtac gtcccatatc
    29101 tagtataggg tctattgcaa gtataccacc aactaatgga aaataagaac caagaccaac
    29161 tccagagata acaactgaaa cggccattgt agcagttcct ggaagaccag ctattccaaa
    29221 cgaactaatt gttataacaa ttaacaacat aacataaaag ctcatatcca tgtttactcc
    29281 agccatattg gcaagtgtta cagccattaa agcaggatat atacctgcac aaccattcat
    29341 acccatattt gcacctaaac ttgctgtaaa gcttgataca ccctcatcaa ctccgtgatt
    29401 ttgtactaga ctttctatcg ttacaggcaa tgttcctaaa ctagaacgtg aagtaaatgc
    29461 cagaagtaaa gcttcagttg aattttttat ataagtaatg ggatttagac catttaaagt
    29521 tataaaaatt aaatgtataa taaataatat tgcaactgat atataaagag ctaatataaa
    29581 ctgtaaaaca gatactattg aactaatacc ttgtgaagtt attgtatttg caagcagagc
    29641 aaccacagca taaggcataa atttaataac agtcatagac acacttacaa ttattttgta
    29701 agatgcttct ataaaatcta taaatggttt tattgtctca aaatgttttt tacttaatct
    29761 ctttattgag gtagctataa atgtagcaaa tattattgta gctacaatat ttccatttgc
    29821 catagccatt acaggattag atggtagtaa tcctcttaaa gtatccacaa gagagtttat
    29881 ttctttaagt tcttgatttt caactaaagt aggcatatta gctcctacac ctaacttcat
    29941 tatatttgca acgataattc caacaattgc agctatagca gtagtagcaa gaagcatacc
    30001 taaagattta aatgtcagag ttccaagatt gttatctttc atatttataa ttacttttat
    30061 aatggataag aaaactaaag gtactactaa catttttaat aagtccataa atccagaacc
    30121 aaataggcta taccacttac ttacttcttg tagccaagtg acttgagttg tatcattagg
    30181 aaaaccagca aaagcttgta ttatgattcc caatatgagt cctagtactg tagcatatat
    30241 tgttctcaat gaaaatttta tttttttctt ttcaaattgt ctaacaataa agaacgtacc
    30301 tagaagtaat attatgaaaa aaattgtttt aaaatctgta atcataagaa attgtgataa
    30361 aaatgaactt tccaatattt ttctcctttt atttaaaaat aaatttttct aagtttaaat
    30421 gatatcaata tattaaaaaa atgtcaatat tatatcacaa tttacttaaa tagagtaaaa
    30481 caagttgatt ttgaaaaatg tgattaagta cagaaaaata gggtataata aatgtttaaa
    30541 gaatttgaat tgtggtaaga aaataaaaaa tagcttttat aagatttgat ttcaaattaa
    30601 aatatgttaa ataaataact aaaaattatt ataaaaaatg ttttcataga atatatatca
    30661 aatgaaaagc ttaaaataag tgaagaaatt aactagacat caaaggagag ataaaatgag
    30721 agtaataata attggtggtg ttgcagctgg tatgagtgct gcagcaaaat taaaaagaat
    30781 aaaacctgag tatgaagttg ttgtttatga aaaaactgaa atagtatctt ttggtgcttg
    30841 tggattacca tactttgttg gtggattttt taatgatgct gatgaattat tagcaagaac
    30901 accagagaaa cttagagaag ctggaattga tttaaatata tttagagaag tcgttgaggt
    30961 tgatagtgaa tctaagaaaa taaaagtaaa aaacataaaa acagatgaaa tatatgaaga
    31021 ttactatgat aaattgatga tagcaactgg agcaagaagt attatgcctc ctataaaaaa
    31081 cataaaattg aagaatgttt ctacattaaa aagtttatat gatggagaat atctaaaaaa
    31141 acttttaagt aatgaagata ataaaagagt gacaataata ggagctggtt ttatagggtt
    31201 agaagcagtt gaggcatgta agaaattagg taaagatgta gatgtaattc agttagaaga
    31261 tagaatattg ccacaagtat ttgataagga aatgacggat gtattagaag aagaaataaa
    31321 tagacataat gttaatttgc acttagatga aatggttgta gaactttgtg gagaagataa
    31381 agtagaaaaa gttataacaa acaaagggga aatagataca gatgtagtaa taattgctac
    31441 tggcgtgaga cctaataccg agtttttgtc aaatactaat ataaaaatgc taaaaaatgg
    31501 agctatagta gttgatgagt atggaagaac ttctgtagaa gatatatact ctgctggaga
    31561 ttgtgcaact ataaagaata tagtaagtaa tgaaaatgta tatgttcctt tagctactgg
    31621 agctaataaa ttgggtagga tagtaggaga aaacctagca ggaagagaag tatcttatca
    31681 aggttcacta tcttcaagct gtataaaaat tatggatatg gaggcagcat caactggaat
    31741 tacagaaaag caagctaaag atttaggcat aaatgtcaaa tctaaattta tttctgatta
    31801 taatcaaact cattattatc caggaagaaa taaaatatat gtaaagctta tttatgatgc
    31861 agatacaaaa gttattttag gcggacaagt ggctgggttt aaggatgctg tccaaagagc
    31921 aaatgtattg gcagctgcta tctttggaaa aatgaccaca aatcaacttg gaatgttaga
    31981 tttatgttat gctccaccat ttgcaagaac atgggatgtt ttaaatgtag ctggaaatgt
    32041 atctaaataa tatttagatt atagctaata tagttgacaa gcaattaagg gaatgaattt
    32101 acctaaacta tttttatcat ggtaaaattc attcctaaat ttttattcca aatttttatt
    32161 ccaaattttt agaaataagg attccattaa attattcatc aaaatttaaa cgcaagctta
    32221 atctatcttc tccaaggata gtattttcaa aaacattagt agcattttct aaatacatac
    32281 taggtatgtc taaagaagga ctaaaatgag tcagtaaaag ttgtttgaca tttccaagtt
    32341 ttgcaagatt agcagcttct ctaaaagtca tatgtttgtt tctaacagct tttgatatat
    32401 ctaaatcatc accatacatg gcttcacata taaataaatc actatctttt atgaaatctg
    32461 gtattgattc tataggtctt gtatcggttg taagacttat cttaactccc tttctcaatt
    32521 ctcctaagac catatctgga gtgtattgtt ttgaatctaa aactatattt tgaccttctt
    32581 ggagtttgtt ccataatatt ttaggaactt cgtttttagt tgccttatct atatcaaatt
    32641 tagggtttct tctaaaatta aaactgtatc ctaaacactc tatagagtgt tctaaactga
    32701 ttgttgaaat ttctaaattt cttaaaactt tattatctaa agaaaaattg ccttgagggt
    32761 tttctataat ttttaaagtg tatggtacat attctactaa atttctcata gaacgtatac
    32821 aatcgactat tccaactgga ccaactatag ttaaatcact tgtacgacca ctatttccaa
    32881 ttgttgataa aagtcctaaa agtccaaata tatggtcacc atgtaaatgg gttatacaaa
    32941 tcaagtctat atctttaaag ccacattttt ttagcttcat agatacctga gtaccttcac
    33001 cacagtcaat tagtattttt ctacctttat agtttataaa tacagaggat agaaatctgt
    33061 ttggcatagg tacatttcca ccacatccaa gtaaagttaa atctatcatt ttttcccctt
    33121 tctaaaatta acacaaatct gttgtttgtt tttattatga tatcctatta taattatttt
    33181 aacattaaat ataacaataa atgtgtattt gagtcaagaa atattgtcaa ataaagtttt
    33241 tatatagaaa tgtataaaaa tttggaaaac aaaaagtgaa aattatattt aggagcattt
    33301 aaaggaggaa aatttaatga aagtattaaa gaaaattact attttgtcat taattacatt
    33361 aaccttagtt ggttgtagta acggagaaga taaaaaagtg gttgaaggtt ctaagaaaat
    33421 tcaaaattct aaaaaagatg aacagcctat aatagtacat aaagatgcaa atatattgaa
    33481 agataggtat gagccaccaa aaggatttaa aagagttgat gtagaaaaag gtagttttga
    33541 atcatttctt aggaatacta agctaaaaaa ttatggagag aaagtaaagt attatgatgg
    33601 aagaactaag gaatcaaaag gtatttatga tagtgtattt gatgttgata ttggcaatag
    33661 agatttgcat caatgtgcag atgcaattat gcttatgaga gcagaatact tgtatcagaa
    33721 taaactatat gatgaaataa gttttgattt tgttgatggt tttaaggcga agtattcaaa
    33781 atgggcacaa ggatatagaa tttcagtaaa agatagtggt tcaagctggt ataaagcaac
    33841 tgaatattca acatcttatg agagttttag aaagtttatg gatatagtgt ttgcatattc
    33901 aggaacgtta tctcttgaaa aagaattgga gcctgtaaaa gttgaggata tgaaaatagg
    33961 agatgtgttt atagttggtg gaagtccagg acatgcaagt ataattatgg atatggcaga
    34021 aaatgaaaag actggtgaaa aggtatttat gatagcccaa agctatatgc ctgcacaaca
    34081 aacgcaactt cttattaatc gtaaaaatcc aaatttgagt ccttggtata gtttagattt
    34141 tgaaggtgat ttgacaatac ctcaatggac ttttaaaaga gaccagttga aaagatttta
    34201 aaataataat tttatataat aaaagaaaaa tata
//
LOCUS       DACULQ010000020        32454 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.020, whole genome shotgun sequence.
ACCESSION   DACULQ010000020 DACULQ010000000
VERSION     DACULQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.020"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..296
                     /locus_tag="JA154_04125"
     CDS             <1..296
                     /locus_tag="JA154_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAT7995046.1"
                     /translation="IVPSRDEFITIYKQALIKKEIDLDMVNIRETFNVIPLKFFTILN
                     VFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNESLILNNLKNLEKQYNSSY"
     gene            309..821
                     /locus_tag="JA154_04130"
     CDS             309..821
                     /locus_tag="JA154_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAT7995047.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            895..3102
                     /locus_tag="JA154_04135"
     CDS             895..3102
                     /locus_tag="JA154_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(p)ppGpp synthetase"
                     /protein_id="HAT7995048.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3127..3576
                     /locus_tag="JA154_04140"
     CDS             3127..3576
                     /locus_tag="JA154_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAT7995049.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3591..4205
                     /locus_tag="JA154_04145"
     CDS             3591..4205
                     /locus_tag="JA154_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT7995050.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4293..5852
                     /locus_tag="JA154_04150"
     CDS             4293..5852
                     /locus_tag="JA154_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HAT7995051.1"
                     /translation="MMLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLI
                     NRLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSES
                     KRNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTI
                     LKDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLK
                     QFGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFN
                     ELDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVS
                     DLVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRA
                     SNLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAK
                     EGYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIK
                     RKKEEVYKNVD"
     gene            5842..7104
                     /locus_tag="JA154_04155"
     CDS             5842..7104
                     /locus_tag="JA154_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAT7995052.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7178..8965
                     /locus_tag="JA154_04160"
     CDS             7178..8965
                     /locus_tag="JA154_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HAT7995053.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMLTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            9424..10656
                     /locus_tag="JA154_04165"
     CDS             9424..10656
                     /locus_tag="JA154_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HAT7995054.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            10670..11476
                     /locus_tag="JA154_04170"
     CDS             10670..11476
                     /locus_tag="JA154_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HAT7995055.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(11588..12457)
                     /locus_tag="JA154_04175"
     CDS             complement(11588..12457)
                     /locus_tag="JA154_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT7995056.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            12829..14256
                     /locus_tag="JA154_04180"
     CDS             12829..14256
                     /locus_tag="JA154_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HAT7995057.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIGSVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(14333..14608)
                     /locus_tag="JA154_04185"
     CDS             complement(14333..14608)
                     /locus_tag="JA154_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995058.1"
                     /translation="MNMKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMRGLSINTL
                     LIVIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(14605..16047)
                     /locus_tag="JA154_04190"
     CDS             complement(14605..16047)
                     /locus_tag="JA154_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAT7995059.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(16072..17238)
                     /locus_tag="JA154_04195"
     CDS             complement(16072..17238)
                     /locus_tag="JA154_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAT7995060.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKYYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(17463..19583)
                     /locus_tag="JA154_04200"
     CDS             complement(17463..19583)
                     /locus_tag="JA154_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT7995061.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSNNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            19816..20217
                     /locus_tag="JA154_04205"
     CDS             19816..20217
                     /locus_tag="JA154_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995062.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQIGK"
     gene            20260..20553
                     /locus_tag="JA154_04210"
     CDS             20260..20553
                     /locus_tag="JA154_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995063.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            20568..21008
                     /locus_tag="JA154_04215"
     CDS             20568..21008
                     /locus_tag="JA154_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995064.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(21100..21573)
                     /locus_tag="JA154_04220"
     CDS             complement(21100..21573)
                     /locus_tag="JA154_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAT7995065.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(21573..22361)
                     /locus_tag="JA154_04225"
     CDS             complement(21573..22361)
                     /locus_tag="JA154_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAT7995066.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            22689..23933
                     /locus_tag="JA154_04230"
     CDS             22689..23933
                     /locus_tag="JA154_04230"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089238.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAT7995067.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            24256..25353
                     /locus_tag="JA154_04235"
     CDS             24256..25353
                     /locus_tag="JA154_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAT7995068.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            25344..26042
                     /locus_tag="JA154_04240"
     CDS             25344..26042
                     /locus_tag="JA154_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAT7995069.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTENLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            26045..27109
                     /locus_tag="JA154_04245"
     CDS             26045..27109
                     /locus_tag="JA154_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAT7995070.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITSMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            27292..27996
                     /locus_tag="JA154_04250"
     CDS             27292..27996
                     /locus_tag="JA154_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7995071.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            27989..29311
                     /locus_tag="JA154_04255"
     CDS             27989..29311
                     /locus_tag="JA154_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7995072.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            29498..30871
                     /locus_tag="JA154_04260"
     CDS             29498..30871
                     /locus_tag="JA154_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995073.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEAFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(31243..32181)
                     /locus_tag="JA154_04265"
     CDS             complement(31243..32181)
                     /locus_tag="JA154_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HAT7995074.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        12277 a         3880 c         5442 g        10855 t
ORIGIN      
        1 atatagttcc atcaagagat gaatttataa caatttataa acaagcactc ataaaaaaag
       61 aaattgattt ggatatggta aatataaggg aaacttttaa tgtaattcca ttaaagtttt
      121 ttacgatatt aaatgtattt agagaattaa atttacttga ctttaattta aattatgaaa
      181 aaaattctgt actcataaga atattgccaa aaccacagaa aaaattagac ttaaatgaga
      241 gtttaatact caataatcta aaaaatttag aaaaacaata caatagcagt tattaggagg
      301 cgtttcccat ggatttaaaa aactttataa ggaatattga tgatttccca aagccaggca
      361 tagattttaa agatgtgaca actctattta aagatggaga tgcatttaaa tatgcagtgg
      421 attctatagt tgaagaattg aaagataaag atgtagattt agtaatagga ccagaagcaa
      481 gaggattttt gatgggtaca cctgtagcat atgctttagg agtaggattt gttccaatta
      541 gaaaacctgg aaagttacca ggtgaagttg agagttatga gtatggatta gagtatggta
      601 cagatacttt agagatacat aaggatgcaa ttaaaaaggg tcaaaaagta gcaatagtag
      661 atgatttatt ggctactggt ggaactatgg aagccgctgc caagcttgta gaaaaattag
      721 gtggagaagt agtttctatg caatttttga ttgagctaaa attcttaaat gggagagaaa
      781 agttgtctaa ctatgatgtt aattctctta taaaatacta gtacttttat aatagttggt
      841 ataacaccaa ctattttcat atatattaaa attttataca aaataggtgg ttatatgcat
      901 gataaagaat tacaagaaat aattgaaaaa ataaaagttt atgcacctaa tgcaaatttt
      961 gaaatggttg aaaaagccta taatcttgca aaaaaagcgc atgaaggaca atatagaaaa
     1021 tctggggaac catactttat tcatccatta gcagttgcaa atattttaat agagatggaa
     1081 ttagatattg aaactataac agcaggttta cttcatgatg ttgtagaaga tacagaatat
     1141 acctatgaag acataaaaaa agaatttggc aaagaagtag cggatttggt tgatggagta
     1201 actaaacttg gtcaaataaa atatcgttcg aaagaagaaa ctcaatctga aaatttaaga
     1261 aaaatgtttt tggcaatggc gaaggatata agggttatac ttataaaact tgctgataga
     1321 ttgcacaaca tgagaacatt aaaatacatg cctccagaaa aagcaaagta taaagcaaaa
     1381 gagacacttg aaatttatgg tggtatagct catagattag gaatttccaa gataaagtgg
     1441 gaactagaag acagagcatt aagatttatg gacccagaag ggtattatga tttagttagt
     1501 agagtatcaa tgaagagaag tcaaagagag gattatattc aaggaattgt tgaacttctt
     1561 aaagataagt ttaaagattt aagtatacca tgtgatgtat atggtagacc aaagcatttt
     1621 tacagtatat atagaaagat gcaaaaaaaa cataagactt ttgaagagat ttttgattta
     1681 acagcagtaa ggatattggt tgataatgta aaggattgct atgcagtact tggtatagtt
     1741 catacattat ggagacctat tccaggaaga tttaaagatt atatagcaat gccaaaaccc
     1801 aatatgtatc aatcattaca caccactgtt gtaggtcctg atggagaacc tttagagata
     1861 caaataagaa ctcatgagat gcataatata gctgaaaatg gtatagcagc ccattggaaa
     1921 tataaagaag gtatctctag caaagagtca aaggttgaag aaaaacttca atggttgaga
     1981 cagatgatgg agtgggaaaa agatttaaaa gacccacaag agtttatgga tgcacttaaa
     2041 gaagatgtat ttaatagtca agtttatgta tttacgccaa aaggagacgt aattgaatta
     2101 ccagcagaat caacacctat agattttgcc tatagagttc atacaaatgt aggtaataaa
     2161 tgtgtaggag ctaaaattga tggaagaata gttcctattg attataagct tcaaaatggt
     2221 aatattgtcg aggtacttac atcatctaat tctaatggac caagtaggga ttggcttaat
     2281 atagtgaaga ccccaaatgc aaaaagtaga attagacaat ttttcaagaa ggaaagaaga
     2341 gaagaaaatg ttgaccgtgg aaatacaata ttagaaagag aatttaagaa gcatgatata
     2401 ccacaaaaag accctcttgt tgaaaaatat atggcattag ttgctaagaa atttaatcag
     2461 ccaagtgttg aggacttaat tgccactgta ggttatggtg gtataatgtc atctcaagtg
     2521 gtatctaagg taaaagacct atatttaaaa gaggttaaaa aagctaaaaa ggaacaacag
     2581 atacaagaag aagatttaaa taaacacaat ataagtgaaa cagaatacag taaaaagaga
     2641 aagaaaaacg catcacaagg aattatagta aaagggcttg acaatatctt agtaagattt
     2701 gctaagtgtt gtaatcctct tccaggagat gaaattgtag gatatattac taagggtagg
     2761 ggagtagcca tacacagagc tgattgccca aatgcaaaca ataatggtga tttcttccaa
     2821 aatagattgg tggaggtttc atggagttca gcaggaaaag gtaagtttga agcggaaatt
     2881 caaataaaag ctattgacag aagaggaatt gttaatgaaa taactcatat tgttgcaatg
     2941 gataaactta gtttaaacgg aataaatgca agaaaaggta aggatagtat tgtaagtata
     3001 aacttacttg tagaggttaa tgacacagag gaattaaaaa ttctaatgaa aaaattaaaa
     3061 gcaattcctg gtgtagaaag tatttataga atgacaaatt agagttaaaa taatagggag
     3121 gaaactatga gagcagtagt gcaaagagta tcttcatcta aagttacagt agatgaaaac
     3181 actataggtc aaataaataa ggggttacta gtattgttag gagtgactca tgatgataaa
     3241 tcaagtgatg tagattatat gattgataaa atattgaatc tgagaatttt tgaagatgaa
     3301 aatgacaaga tgaatttatc tctaatggat ataggtggag agcttttagt agtatctcaa
     3361 tttactttat atggagattg cagaaaagga agaagacctg gatttagtaa tgcagctaga
     3421 ccagaacttg caaataacct ttatgaagaa tttgtaaaaa aagcaaaaga taaaggtgtg
     3481 acagttggta caggtcaatt tgcagctcat atgatggtag agttaactaa tgatggtcca
     3541 gtaacaatac tattagactc aagtaaatct ttttaagggg gaaaacttta atgattataa
     3601 aaaaatttgt agatagttat tttggagtaa atacttatgt attaggtgat gaaaagacta
     3661 aaaaatgtgc agttatagac ccaggtggaa gtttagtaga gacattaagt tttgtaaaag
     3721 aaaatgaatt aaatattgaa tatattatcc ttactcatgg gcatggagac catataggat
     3781 atgttaagga tattaaagaa aaaactggtg ctaaagtagt tgcacatgtt gatgaaaaag
     3841 aattattaaa tgacaaaaat aaaaatctaa gttatacaat gagatgtgga gcgcaagagt
     3901 ttgatgcaga tatctatgta aatgataaag acaagcttga acttggtgaa ttaaaaatga
     3961 ctttcttgca tactccagga catactcaag gatgtatgtg tataagagtt gaagatgaaa
     4021 tgttcacagg agatactttg tttgctggaa gtattggtag aactgattta tatagtggag
     4081 actttaacca aatggaaaaa tcacttaaga aattatgtaa atatgaagat gctgttagag
     4141 tatatcctgg acatggacca accagtactc taggggttga aaagaagaca aatccttaca
     4201 tgtaatttta tattagtatt taaaaaagta taaaatattt aataactata ttagtgaggg
     4261 ggttcttcta ctgttaaata gaaggagtta agttgatgtt gtatgttttt ttaaaaggac
     4321 atgattataa gtatgaagta gcagaactta taaaattgtt tactagtgaa tttaagttta
     4381 aagactctaa tgctaacaat aatatagagt caaagcattt aataaataga ttgatttatg
     4441 agaatggtgt attattctca agtactgaat actatgaaaa tggaaactta aagtacgagt
     4501 caatacagaa tatagaaaat atgaatcttg aatttttaga tggatttgat agcagcaaaa
     4561 cgattgaaga atttcttaat agcctagata gtgaaagtaa gaggaacttt aaaaagctgt
     4621 gtaaagaaaa tatcaaaaaa agcatgtttt tagtgcttaa acaagtattt aattcttatg
     4681 taccatgggg aattttgact ggtattagac cagtcaaaat tgtccacaat ttaatggata
     4741 aaaagctaga tgataatagc ataagaacca ttctaaaaga taattatttt ataatagatg
     4801 agaaaataga tttagctctt gaaatagcta aaagagagcg attatttatg tatcctatag
     4861 ataaaaataa aatttctctt tatgtaagta ttccattttg tccaactaga tgtgtatatt
     4921 gttcatttcc ttcaaattca ttaaaacaat ttggacattt aaaaagagaa tatgtttata
     4981 aattgattga agaaattaag gggtttgcca aagttattaa ggatactaag aaagaagttg
     5041 aaactctata tattggtggt ggaactccaa ctacacttga tgaggaagaa ttggatttgc
     5101 ttataaattc attgttcaat gaattagatt taagtaaaat taaagaattt actgtagaag
     5161 caggtagacc tgacactatt acagaacaaa aactaagagt gttaaaaaaa cacaatgtta
     5221 gtagaattag tataaatcct caaactatga atgatgatac cttggtaaag attggaagag
     5281 agcatagagt atctgattta gtagattgct ttaatatggc tcgtagaatg ggatttgata
     5341 atataaatat ggatataatt ttaggtctta tagatgaaga cttaaatatg gtaaagaaca
     5401 cattggagga gataaaaaaa ctttctcctg aaagccttac agttcataca ttagccataa
     5461 agagagcatc taacttaaat ataaatatgg ataggtataa agaacatcta acacaatatg
     5521 aagaaatggt aaaaatgata gatttatcaa tgaaatatgc aaaagatatg ggattaaatc
     5581 catattatat gtacagacaa aaacatatgt tgggaaattt agaaaatata ggttatgcta
     5641 aagaaggata tgagtgcatt tataatatac agattatgga agaaaaacaa agtaactatg
     5701 cattaggtgc aggtgctata tcgaaatttg tctatgtaga tgaagataga attgaaagag
     5761 tagaaaatgt aaaaaatgtt gaacaataca ttgaaagagt agatgaaatg ataaaaagga
     5821 agaaagaaga ggtatataaa aatgttgact aaagctccta gaggaactaa agacataact
     5881 ccaaaagagg cttacaaatg gcgttatgta gaaaataaat ttagagaaat atgtgcttta
     5941 tatggatatg aagaaatggt aacacctata tttgaacata cagaactttt caagagaagt
     6001 gttggagata ctacagatat agttcaaaaa gaaatgtatt cttttaaaga taaaggtgat
     6061 agagaaatta ccctaaagcc agaaggtaca gcaggtgtag taagagcatt tatagaaaat
     6121 aaattatatg cagatactca accaacaaaa ttattttatg tgacaccttg ttttagatat
     6181 gaaagaccac aagctggtag acaaagacaa tttcaccaat ttggaataga agcacttgga
     6241 agtgatactc cttcaatgga tgctgaaata atcgcactag ctgttcaatt ttttaatgaa
     6301 gttggattaa atgatttggt agtgagtata aattcagttg gctgtccagt ttgtagaaaa
     6361 gagtacaatg ctttattaaa agagtatctt gactcaaaag ctgatatatt gtgtgatact
     6421 tgcaatgaga gaagagaaaa aaatccaatg agggttatag actgtaaaaa tcctacatgt
     6481 aaagaaaata tcaaagatat accatttatt gcagaccatt tgtgtgatga ttgtaaatct
     6541 cactttgata aacttcaaga atacttaaag gaaatgaata taaactttgt aattgataaa
     6601 acaatagtta gaggcttaga ttactataga aaaactgctt ttgaaattat atctaatgat
     6661 ataggagctc aaagtactgt atgtggtgga ggaagatatg atggtcttgt tgaacaatta
     6721 ggaggaccaa aaggtataag tggaattggg tttggactag gaattgaaag acttttactt
     6781 acacttgaag gtaatggaat tgaaatagaa aaccctcaat ctacggatat atttatagta
     6841 actataggtg aagaagcaaa tacaagaagc tttaaattgt taaaagactt gagacaaaat
     6901 catataagtg cagataaaga ccatatagag agaagtgtta aagcacaatt taaatattca
     6961 gataaaataa attcaaaatt tacaatagta attggagatg atgagcttaa aaatgacact
     7021 gcgacattaa aaaatatgaa aacatcagag caaactactg taaaattaag tacattagtt
     7081 gaagaattaa agcaaaagct gtaaaataag aaagtatgat gttttgtgta ttagataaat
     7141 atatagcatt aaaattaaat aaaatggagg tagtatcttg gaaactttaa agggtttaaa
     7201 gagaactcac tactgtggag agttgagaga aaaaaacata aatgaagaag tcgtacttat
     7261 gggatgggta cagaaaaaaa gaaatttagg tggtcttgta tttgttgatt taagagatac
     7321 aagtggatta tgtcaaatag tttttgatac agatgtagat aaagaagctt ttgaaaaagc
     7381 tgaaaaatta ggggctgaat ttgtaatagc tgtaaaagga aaagtatgcg aaagacaatc
     7441 taaaaaccct aatatgctaa ctggtgatat agaaatattt gcaactgaac ttagactttt
     7501 aaacaagtca gaaactccgc ctatttatat aaaagatgat gatgatgttt ctgaagcact
     7561 aagattaaaa tatagatact tagatttaag aaaaccatct atgcaaagaa atttaaaatt
     7621 aagacataaa gtaatgaata ttacaagaaa ttacttatca aacaatagat tttgcgaaat
     7681 agaaacacca tttttaatag ctccaacacc agaaggagca agagattatc tagtaccaag
     7741 tagagttaat ccaggaaaat tttacgcatt accacaatca ccacaattat ataaacaatt
     7801 attgatggta agtggtatgg atagatattt ccaaatagtt aaatgcttta gagatgaaga
     7861 cttaagagca gatagacaac cagagtttac acaaatagac tgcgaaatgt cttttgttga
     7921 gcaagaagat gttatgagta tgattgaagg tttgcttgaa gcgatattta aagaagtttt
     7981 agatgtagaa ttggcacttc cacttcctaa gatgacttat gcagaagcta tgtctaaata
     8041 tggttcagat aaaccagata ctagatttgg atatgagtta acagatatat ctgatgttgt
     8101 ttgtaactgt ggatttaaag tgtttgctga tgctacacaa ccaggaaaaa gtgttagagg
     8161 tataaacgta aaaggcaaag ctgacgactt tactagaaaa caaatatcat cactagaaga
     8221 acatgctaag acatatagag caaaaggtct tgcttggatg aaagtaggac aagaaggagt
     8281 aacttctcca atagcaaaat tctttaatga agaagaaatg aacgctatac ttacaagaat
     8341 gaatgctgaa gtaggagatt tacttttatt tgtagctgat aaaaactcaa tagtatttga
     8401 tgctttagga caagttagac ttgaagtagc aaataggctt aatctattgg ataaaaatgt
     8461 atataactta ctatgggtaa ctgagttccc agtttttgaa gaagatgaag aaacaggcac
     8521 attctcagca atgcatcacc catttacatc acctatggat gaagatttag acaagttaga
     8581 agaaggtgat aagtcatctt taagagcgaa agcttatgat attgtattga atggttatga
     8641 aataggtgga ggaagtgtta gaatatcaaa ttctgatgtt caatcaagaa tgtttaaagc
     8701 acttggtttt acagaggaaa gagctaatga aaagtttggt tatttattag aagcatttaa
     8761 atatggtact cctcctcatg ctggtctagc ttttggtctt gatagacttg ttatgttact
     8821 tgcaggaact gataatataa gagaagttat tgcattccct aaaaatcaaa atgctgtttg
     8881 ccctatgaca aatgctccta cattggcgga agatgagcaa ttagaagaat taagtataaa
     8941 agttgatata aaagataatg aataatatgt agttgcaaga aactaaaatg taaaaagtct
     9001 ggatataaaa tatatttata ttcagacttt ttttattaga attattttat attttttctc
     9061 tatatatttt cataaattat acttatattc ttaaccaata taattaaaat actatgttta
     9121 aaaattttac ataaacttga ttattttaga aactacttct gtaatgagta cacgatttta
     9181 attaaagaat atataacaaa tttgaaataa gtctaatata tgacttaata gattaaatta
     9241 aaataataaa tttggactat ttatctattt ataacttaaa ttatatcaaa ttgataacaa
     9301 actagatatt atactttttt gtagaataga attacaatta tataaagaaa aattctatta
     9361 atagtgaaaa aaaagtagaa aaaagtagaa attattcttt tataaagaaa gggagggata
     9421 atattgcaag agagagaaga attagtttta aatccagttc caaaagagga aagagttgga
     9481 tggttagctc cattgtttaa tatgcttggt tctaacatag ctatatctga actaatggtt
     9541 ggaggaactt taattttagg aatgactctt tctaatatga ttattacatc aattataggg
     9601 aacttaatat tagttgcaat tattatgata caaggttata taggttacaa agaagggtta
     9661 aatacgtatg tattagctaa gggggcattt ggagagatag gaggaaagta tcttatatct
     9721 ctattacttg gtataactag ttttggatgg tttggtgtac aagcaggggt tgcaggacta
     9781 tcagtacaaa agatatttcc aagtgtaaat ctaacattag ttacagttat acttggatta
     9841 tttatggttg tttttgcact gtatggcttt aaagctatgg ctaaatttaa ttacttagtt
     9901 ataccaccac ttatgatatt aatggtgtgg ggagttttta aagctttttc tacttatgga
     9961 gttgaagcta tttataatta tactcctcaa acaactatga gtatggttga aggtcttaat
    10021 attgtagtag gtcttgttat agttggtgca attatatcac ctgaccagtt aagatatacg
    10081 agacgagtaa aagatatttg gattatcagt tttttaggtc tagggattat ttcattattc
    10141 caacaagttg cagctggtgt aatgtctatg ggagcaccta cttgggatat aacagaagta
    10201 ttggctaatt taggttttgg atgggtagcg tttgttattt taatacttgc atcttggtct
    10261 acaaacgtat caaatgctta ttctggtgga ttagcattaa aaactatttt tccaaatgta
    10321 aaaagaaatg ttttgacttt agttgcaggt ctaataggta caataatagc agctactggt
    10381 ataatattta aatttcagtc atttttatct ttcttgggaa tcgcagttcc tgcaatagct
    10441 ggaattatgt ggtgtgagta ttattttata caaggaagaa cttataagca tagagaagga
    10501 ataaattgga ttgcagtaat aagctggtta ataggttttg cagcgtcgta ttatagttca
    10561 aaaataaatt tcttaatacc tccgataaat ggaattgtag tgagtatggt tatttattat
    10621 atattaatga aatgttttgg aatcaaagat aaataaaggg ggcaattaca tgggtcgtaa
    10681 ggtgaaaata ggtataattc aacaacacag tgtacttgga gatgttaaaa aaaatataga
    10741 aaaggcagta gagatgatag atgacttagg aaaacaaggg gcagatataa tttgtttacc
    10801 agaactattt gctacaggat ataatttaga gtctttaggt ggagttaaaa cattagaatt
    10861 aataagagaa cataataagt acatagagga atcaatgtca gaagctgcta aaagaaataa
    10921 tgtatacctg atatctccat atggaacctt agaaaaaggg tctacacatg tttataattc
    10981 agcagttata tttgatagaa aaggtaagat tatgggagaa tactgtaaaa atcatctgtg
    11041 gtcattagaa gcagtatact ttaaaggagg ggaaaaagta gaggtatatg atgctgattt
    11101 tggtagattt ggtgtaatga tatgttatga tgcaggtttt ccagaagtat ccagagaatt
    11161 gacattaaaa ggttctgaaa taatatttat accatctgca tggagaatac aagatgaaga
    11221 tatgtgggat ttaaatgtat ctcaaagagc tttagagaac actgtatata cagtaggtgt
    11281 aaatttagta agtaatgact ctaatttaat actatttgga aagagtaaaa tatgcaatcc
    11341 tagaggtact gtaataactc aattagatac atacagggaa aaatatgtat tatcagagat
    11401 tgatttagat gaaataaaaa agtataggga agatatcccc tatttgaaag acagaaaaag
    11461 tagctataac atataattct atactataat acaagttcaa tataaaatga taaaaagagg
    11521 atgtatcaat attaagatta aatttattat actttgatat atcctcttta atattatcaa
    11581 ctttgattta aatattttta aaatagttga taagaaagtt tttgaacaac aacacagctt
    11641 tagattcata gttttcgcta aaccatgaaa gatttaaata tcttttaaat tcaatatctt
    11701 tgatatgtaa caacttaatc tcagggtctt gagataatcc ccatgatttt ccacatataa
    11761 atccaactcc ttgaagtgat tttataagag cataaattgt atatggtgaa tcagactcaa
    11821 atgctatttt gggtttgaaa ttagctgatt cgcacaggat atcaattacc tctctataat
    11881 tctcaccctt cgttataact ataaagtttt catctgaaac ttcgcttaga tatacttcat
    11941 cctttaatga taaaggatgg tttattgaaa ctcctaatag tatttcttcg catgttaaag
    12001 ttatgctatt ttctgagttt agctttgtaa atgaagaaga tatgtacaaa tcaaaatcac
    12061 tttttttata agatgatggc aaagtatgtg acacattaaa agtaatattg ggatatagtt
    12121 ttctgaaatt actcaaaagt gatggtaaca cattagccgc tgcaggagat aatatcttta
    12181 cttctcctga attttctaaa gaagtgtctt gtaactcctt ttttgaactt tctactaaat
    12241 ctaaaatgct atctactctt tttagaaaca atcttccata ctcatttaaa acaatatatc
    12301 ttccttttct gtcaaataac tgaactccaa gttctttttc taaggaagat atagttttgc
    12361 ttaaagctgg ctgtgctata tttaacttgt gtgatgcttt tgttatgtgc tccattctag
    12421 caacagtttg aaaatactta agatgcaata aatccatagt ataaaataca cctcttttaa
    12481 tatgacttga gttatatcaa ttttataatc aactccacat tatacattat aatataaagt
    12541 ttatataata aactacaata tattaaaaag ttatttaaat tgttatttat attttgtaca
    12601 taatctaatt aaaaaaagta actttaaatg attaactaag gaaatgttgt tacaaacttt
    12661 agttatataa aaaattcatt tgatgagcaa agtactaata tcaagtaaga ataaaagctt
    12721 attagataag aaaaataata ttttataata atgatgttac ttaaattatt atgttatact
    12781 attttaaata ggaaaaattt agatactata attttagggg gagtcaagat gaaattaact
    12841 aaatcaatat catcaatgct gtgcttatct ctagttttaa gcttatcacc agtagcatat
    12901 gcaaatgaaa taagtagaac aacagttgag aatactttag taggtcagga tagatatcaa
    12961 actgctgttg aagtaagtaa aaaaggatgg agtagtgcaa atgaggctgt agttgtaaat
    13021 agtgaatcta ttgtagatgc attagctgta acaccatttg ctaaattaaa aaatgctcct
    13081 atattattga cagaaaaaaa taatctaaat tcacaaactg agaaagaatt aaaaagatta
    13141 ggagttaaaa aagtttatat aattggtcta tctagttctg tatcaaatga tgtgcaaaaa
    13201 cagttagaat ctaataattt aactactgat agagttggtg gtagtgatag ataccaaaca
    13261 gctttgagta tagctaaaaa aatagaagga ttaaaagata tatcagaaat agctgtagta
    13321 aatgggtaca caggtcttgc agatgcagtt agtataggtt ctgtagctgc tactaatgga
    13381 atggttatac ttccagtatc tgatgcatct ggtatatcta gctttaaaga ttttatagaa
    13441 tctaagaata taaataaatc atacataata ggctctacta atgctgtatc agataaaata
    13501 aaacaaagtt tacctaattc agaaagaata ggtggagcag atagaaatga aactaatggt
    13561 aaagtaatag aaaagtttta tacatctaat aaattaaaca atgtatttgt ggctaaggat
    13621 ggaatgaaaa atcaaaatca acttatagac gctctagcag taggagtact agcatcaaaa
    13681 gaaaattctc ctgtactaat cgtaggagaa caattaagtg caacacaaaa gagtgtattt
    13741 tcgaataaaa atgctacctc tataactcaa gttggtagtg gaggaaatga aaatgcattt
    13801 aaagaattaa aagatttaca ggcaaatagt aacaataaga gagtaatgta tgtaactaat
    13861 acagataagg taaatataag aaatgatgct acaatagaag cttctgttat aggatatcta
    13921 aataatggtg atgaagtaga agttttagat gtgttaaaaa ctggatgggt aaaaataaaa
    13981 tacaatgaag gtattggata tgttagtggg tcttatttga caaataataa gcctgataat
    14041 agtaatgaaa atataaaaat aaagtatgta aaagaaaaag atggtctaaa tgttaggaaa
    14101 ggaccaagta cagaagatga aaaaataggt cacctttcat atggaagcaa agtagaaaca
    14161 atagaaatgt ttgctactgg atgggtaaaa ataaagtaca atggtggata tggttatgta
    14221 agtaatgatt acttatctga tacaccagtt atttaattat aagtaacaat aaaaaaactg
    14281 ctttaatttg taaagcagtt tttttataaa aatttaagtc taataactat ttctattttg
    14341 ataccttaat cctgggattt attattatgg atattatcaa catacaaaca ctccaaatcc
    14401 agtgtctaat tccaaactta gatattaata aattcagtcc tgctatggta aataagcagt
    14461 atttaattac aattaaaagt gtatttatag aaagccccct catagaaagt atcctaaatc
    14521 caataataga cattctaagt cctataaatg ccaataatat acctgcaata tcctgaatta
    14581 agtaatagta ttcaaatttc atattcaatc aactcttctc tgagcagtat caaatatttc
    14641 taactcttca atttcatcat ctgtgtatgt aagcatagta aaaccagtta caccataaat
    14701 aaatgaaatt ataggtgtaa tccaacttaa gaaacaatat ggaataaatt ctagtggact
    14761 tacaccaaga gtctgagcac agaaaacagc atttgaattc catggtatta atggaccacc
    14821 aagtgtagct gtatcttcta taattctaga tagattttgt ggttttaatt taaattcttt
    14881 gtatattgga gacattagag ttccgacaaa tacagaagtg aacatcattg atgaactaaa
    14941 tgcatttgca aagtagctta ctataaaagt agacccaacc aatttagttc ttgattttcc
    15001 tattttagtt ataagaggtt gaagcagtac ttctaagaat ccagtttttt ggagaatacc
    15061 agcaataacg aaaactacaa atactagtaa aacggtttca gccatactca ttattccacc
    15121 acgatttaat agtttgtcgg catagacaaa tccagtatct atagtaaatc ctgatagcat
    15181 gtaatttaaa acatcaccta ttttttctcc ttcttgaaat actgctataa taagtcccat
    15241 gatagctgaa cttagtatag atataatggg tggcttttgt agcaataaaa gtaataataa
    15301 aaatattatt ggaatcattg ctactaaacc aatatgaaag tttgaattta atacatcctt
    15361 gatttgattt atttgaacat agtctatagt attatttgaa tacttaaatc caattactgt
    15421 atacagaact atacatatta cgtatgcagg tcctgtggta taaaacatat gcttcatatg
    15481 tgtaattaca tctgtcttac aaactgctgc agctaagttt gtagaatctg atagaggtga
    15541 catcttatct ccaaagaaag caccagaaat gactgcacca gctgttaaac caagaggaat
    15601 acccatacta gtaccaatac tcatcattgc tataccagaa gttccaagag tcccccaaga
    15661 tgtaccagta gcaacagaag ttaatgaaca tacgattaat gttgtaagca gaaaatattt
    15721 aggggttatt attgttagtc cactatatac tactgttggt attgtaccag cagctatcca
    15781 agctgctatt agtacaccaa cagaaagtat aattaagttt gattgaaagg catctttgcc
    15841 aacttcaaat ccaaaagctt ctatttcatc attagtataa ccaagtttca tggctgcagg
    15901 aactactata agccatgaaa gtaaaaacat tgtagttata gaagcattaa atacaactat
    15961 accaactgat gttagagcta ttattgcaag cattactaac aaggaatatc taaatgaagg
    16021 tatttttctt tctgtattca ttttcatagt gcaaattctc cttaaatatt tttagttaat
    16081 tgcatttttt attcttataa gagcttcttc aagcatactt cttggacaag ctaagtttat
    16141 tctttgatac ccacttcctc caatgccaaa gctgtttcct tgatttagag caactttacc
    16201 tttttgtaca agtatacttt ctagttcctc atcacttaat ccaagtgcac taaagtctac
    16261 ccataataaa taagtacctt ctggttttct aactttaagt tttggcatat tttcatttat
    16321 atattttata gcaaaatcta tattagattc taaatattct aaaaatgatt ctagccaaga
    16381 ttcaccatta ttatatgaag cttcagttgc aactaagcta aagcaattat ttctctttat
    16441 atcaattctg gtaaaggcat catctaacaa tttatagtat ttttcatcag gtagtattac
    16501 atatgatgat tgaagaccag ctatattaaa agttttagtt ggagccatac aagtaattgt
    16561 atttttttca aattctttag aaatagatgc cataggaata tgcttatgtt tttttaatat
    16621 aatgtcacta tgtatttcat cagatatgat ttttacatta tgcttaagac aaatatctcc
    16681 tagttttttt aattcatctt ttgtccatac tctaccaact ggattatgag gattacaaag
    16741 tatgaataat tttacatctt taatcttgtt ttcaatgtct tcataatcca ttatgtagtt
    16801 tccattttct aatttttgta atgggcttat aattagttct ctattgttat tttttacaac
    16861 actattaaaa gggctgtaaa ctggttcttg tatcattatt ttatcatttg cttttgtgag
    16921 ttcatttatt aaaagactta tagctgggat aacacctgga ctataaatta accactcact
    16981 ttttattttc cagttatgtc tcctatctag ccagtttact atagattcat tgtaagaatc
    17041 tggtcttgtt gtgtagccat aaatctcttg ttctagtcta ttttttaaag aatcaattat
    17101 acaaggtgca gccttaaaat ccatgtcagc cacccacata ggaagtaaat cattagttcc
    17161 atactttttt tccatttctg accatttaga tgaaaaatta ttgcttctat caacaatctc
    17221 attaaaatta taattcattg taaatacctc cagtaatttt aattttatgt taataatatt
    17281 aagcaaacaa cgtgccaata ttgataataa aaaatattta ttcacaagtt aatatttaat
    17341 attaagtaat ttcagagcat agataagata aaaatattaa ttatacttaa aactttgtgg
    17401 aaaaggttat ttctaataaa tttttaaata tatgaaaatg taattaaata ggttaaatag
    17461 gtttaactgt ttaacttgtt agacctattt atatttttta gaaactctac tccctttgat
    17521 gttataattg aacctccacg accaacaagt atgtctacaa gcccaaagtc ttgtagttct
    17581 aaaagcatat ttctaatctg ttgttctgtc aaaaatctat tttcctctaa agctatttta
    17641 cataagcttt ttctacctat tctttctttt aaatcataag ccttttttaa attatccaat
    17701 ataaatatgt attcatctag tgacctttta aaattatagt cgtatttcat gatgtttttg
    17761 tctttgccaa tgttgaattg atactttttt atattattac ttactttatt acaaacggtt
    17821 ctactataat ttgaatctat agtatctaat atatattcag gtaaatcaca tatttctata
    17881 atatcttttt ccaagtaaca gaaatattct cctaagtttc ttaattctct tacatttcct
    17941 tcccatctat acattttaaa gatttctttt acttcctcac ttagaataaa attacatgga
    18001 atatcatatt ttaatgaacc aaaaatttca aatatatcag caggtctttc cctaagtgga
    18061 tgtattttaa ggggaagaac attcaaccta aaatataaat ctcttctaaa catattttta
    18121 gatacaagtt cttttaaatt tctattagta gctgcaatta ttcttacatc aatgtcaatg
    18181 acattattag aaccaatacg tcgtacttgt ttttcttgta tgactctaag taatttagat
    18241 tgagaattta aatccatttc tccaatttcg tctaaaaata tagttccatt gttggctaat
    18301 tcaaataaac caattttccc acctttttta gcaccagtaa aagccccttc atcatagcca
    18361 aataattcac tttgcagtag attttcttga aaagtactac aatttactgc gacaaaagga
    18421 ccatcttttc tcctagaagc actatgaatg gattgagcaa ataattcttt accagtacca
    18481 ctttcaccaa ttataaggat agatgaattt gattgagcca ttttattagc aatttttttt
    18541 gtattaatag tttgtatact agaacctaat atatcatcaa atgtgtattt actgatatta
    18601 ccagagttta ctagttgtgc tcttaattta gcttgctttt tttctaattg agaaaacttt
    18661 gtcatcttta gtataaatcc atcaaataca tttaattgta tatgtttaat ctctaaattt
    18721 atatcaatat gatttatttt aatcaatttc tcaaaaaatg gagttttatt ggtcaatatg
    18781 ctttgaaaat tgatgtcttt tatgcaatca ccaacaaatg agtcaatcat ttcatttgca
    18841 tttatagaca aaatttttct agccatatga ttatagaatt gaattattcc atcgttattt
    18901 gtgcatataa ttccatcatc aattatagat agtaacaaat caaattgtct ttcaaactta
    18961 tttgcatcta acaataactc acctgtacta taacttgtag gaattatttt ttctacgtat
    19021 ttttttatta ggtcatcttt tattagatgt tttaatttgg tttttgtagc aatttcaaca
    19081 atacaactta aatctattat tctgtatttt aaatctataa cttcacaatt tttaaactta
    19141 ggcaagattt tttcgccagg agttaggatt acagcatctg tgggagtaaa ctcaatctct
    19201 ggatagcagg gcaggatatt tatattgtct attcctaatc tatgtattaa agaaattgtt
    19261 tctattgcca tatctttact taagttatat acataaataa ttttatcttt ttctaatttg
    19321 gatatcatat caatactgtt cttttcaaat gtcaacttag gaacaatgat ttgagcatca
    19381 tagtgagata attttactat ttcatcatat tttaaataag cagataggag tataagtttt
    19441 tctttaaaaa actttaaatt tccttcttca aatgagtata aatttatctt tagattatca
    19501 cctaagaatt gtcttatttg attttcataa atttctccag cgtctttttt taaacttaca
    19561 atagctaatt ccatttgttt cattttaacc tctcatacaa atttgactta ttttagatta
    19621 cataataatt aaattagatt aaataatatt atttaattat tattataaat ttattataga
    19681 ctacacattg aagtaaaaga agtagctaaa attattcctt ttatttttgt tttttgtatc
    19741 tatttggtat aatagtatta gagcgtataa taatttaggc aaaatttttt aaggaagact
    19801 agaggagaat ataaaatgaa aaaatgtctt tactgtaata gggatataaa gtcaccaaat
    19861 aataaattag ctataaaata tgatgacaat atgaatatat atccatgtcg tgcagaatgt
    19921 aaggaaaaaa ttgaagaata tatagcaaaa aaacctacat ataagtttat aaatatatta
    19981 gaggttcttt taggtgtggg cggaatattc tgctttttga gtaaatggac tattgcagcc
    20041 caagttgtac ttggaattga tgcactggtt atatttttat tcccattggc aggatttaag
    20101 atgaatggaa agttaagtat ggaacaaact aagaatcaaa gtaggtctat tgcaatatca
    20161 attttggcat ttatagtaat aataactgta ttatatttta aacaaattgg taagtagagc
    20221 ataattgtat aagacatcat tagagtatga ggtgagttta tggaagaaag aaaattagca
    20281 ccaaacaatg agagggaagc tctcataaaa agattaaaaa gaatagaagg tcaagttaag
    20341 ggtatacaaa agatggtgga acaagaacgt tattgtgttg atatattggt tcaaatatct
    20401 gctataaggt ctgctataaa tagagtaggc acaattatct tagagaatca tataaaagga
    20461 tgtgtagcag aaagcattaa acatgatgat gctgataaaa ctgaagagat gataaaagag
    20521 cttatggata ctataaataa atttacaaaa taaggaggcg gttagttatg aatcaacaag
    20581 gatttattat ggaaattgtt gatgatagaa ctgcaaagtt aaaaatgcaa agacattcag
    20641 cttgtgctgc ttgtggaaaa tgtgctacta caaattcagc agagtcaaaa gaaatattag
    20701 tagaagtaga caacacaatt ggcgcaaaag ttggtgacca tgtagaagtg agtatggata
    20761 atatgaatgt attaaaagca gctgtaatgg cttatataat acctcttata gcacttcttg
    20821 taggaacaat agggacttat tatatattgg gggcaattgg aatgacaact ggagttgaag
    20881 ccataagtgg attagttgga atttgtctta caataatctg ttatttatac ttaaagaaga
    20941 atgacaaaaa atttagagac agtagagaat ttataccagt aataacaaga atattaattg
    21001 acctttaaga taattataat taaaaaatag caatgtgctt cttaaataaa tactcaatac
    21061 gtatttgtag agtttaagta cattgctatt tttatatttt tattttgatt ttctgtaagg
    21121 aataaagttt tttctgattt tcaaaagatt caatgttctt acaaaagaag ttacaactaa
    21181 tacaccaagt gctattgcag cacttagaaa tagcgcatct gtaccttttg acatagccaa
    21241 agaataattt ttctgaatca aagacaacat tgtattatat agcccaattc ctggtattag
    21301 aggaagaata cctggaatta cgaacacaat agctggttgc tttagttttc ttgctaaagt
    21361 ctcactgcac gctgagacca aagcagcagc gataaaacct gcaaaaactg catttgtagt
    21421 aaaattaaat aaataaacat atacaatcca accaattccg ccagttactc cagctggaag
    21481 aagtagatat agtggagcat taaagaaaac agcaaatcca gcagttgcaa cacctgaaaa
    21541 taaaaaatgc aaaatcatag acatatcagt cattagaaag gacctcctat tctataccat
    21601 gtatcaagga caaaaccaac tccagaagca attgcaataa taatcatagc agcatcaaaa
    21661 gcacgagaaa ttcctgatat taaattccct gatattaaat ctctaatagc ttttataaaa
    21721 gcaactcctg ggagtagtgg cattatagcg ccgactataa gcattttagg gtcatctaaa
    21781 atgcctattt caactagtag aactcctgaa agtgcaatta aaaaagaaga tactaaacag
    21841 gaaaatgcag atattgcact tatcttcatc gttttatcat aagtaattgc agcaaagatt
    21901 gctattataa acgtaagtat aaaatttaat acataattcc ctccaagcaa acaagcaaaa
    21961 caagcagatg caattcctgt acaagagtaa aataaccata aaggataagg ttttacatct
    22021 tggatttcat aaagacgtgc aatggcatta tcaatatcat actctttgtc actaacaaat
    22081 tctcgagaaa aactgtttaa taaagaaatc ttatttaagt ttatacctct tgattgaatc
    22141 gttttcataa acgaaaagcc atcaaatttc tcatctgaaa taattattac tgtaggagtt
    22201 gtaaaaatat ttacatgata aaaccctctt gacctgcata ttctaaggac actgtcttca
    22261 accctagatg tttcagctcc attttttatc atcagttccc ctacaaatag agctagtttt
    22321 agaatatttt tcttatattc aagttcctca ttttgattca taaatacacc tccaaattaa
    22381 aaaacgtaat attgaaatta tatcaaattt ttaagtaata ttccttaata tctatacaaa
    22441 tatattatct tttttttgtt tcaaaatagt cacatttaaa ttatatccaa ttaaaagaat
    22501 tagacaatat tatttattat attttaactt aaaaataaag aaagtaacta tgttaaatat
    22561 ggaaaaatgg caaaacactt gactattaat tggtaaagga ctataataat aaattatcat
    22621 attgataaaa acgaaaatta tgaaatgtct gtaaaaataa aatttaaata ataactagga
    22681 ggagtaatat gtttgagaac ttgaaaaaag cggaccctga gatttatgaa agtatgaaaa
    22741 gagagttaaa aagacaacaa agaaatattg aattaatagc atctgaaaac atagtatcag
    22801 ttccagtaat ggaaactatg ggaagtcatc ttacaaataa gtatgcagaa ggttactcag
    22861 gcaaaagata ttatggtgga tgtgaataca tagatgaagt tgaaactctt gcaatagata
    22921 gaatcaaaaa aatatttgga gcagaacatg ctaatgttca acctcattct ggagcaaatg
    22981 caaatattgg tgtatatttt gctatgttaa aaccaggtga tgttgttatg ggaatgaatt
    23041 tatctcaagg tggacattta actcatggtg ctccagttaa tatttctggt caatattata
    23101 aattttatga atatggtata gataaagaca gtggacttat agattttgat gaagttagaa
    23161 agatagcaca tgaagtaaaa cctaaaatga tagttgcagg agcaagtgca tatcctagag
    23221 aaatagattt taaaaagttt agagagatag ctgatgaggt tggagcattg cttatggtag
    23281 atatggctca tatagcaggt cttgtggctg ctggtcttca ccaaaatcca tgtgaagtgg
    23341 ctgattttgt tacaacaaca actcacaaaa ctttaagagg acctcgtggg ggtgttattc
    23401 tttgtaaaga aaaatatgct aaagacatag acaaagcaat attccctggt attcaaggtg
    23461 gacctcttga acatataatt gcttctaagg cagtttgctt taaagaagct ttaagtgatg
    23521 agtttaaaga atatcaggta caagttgcta aaaatgcaaa agccttagct gaagaactta
    23581 ttaaaagaga ttttaaattg atttctggag gtacagataa tcatttaata ctattagatt
    23641 tgactaataa aaatataact ggtaaagcag cagaaaagag attggatgat gcttatataa
    23701 cagctaataa aaatacaatt ccatttgacc caaatggtgc tcttgttaca agtggaataa
    23761 gacttggtac tccagctgta actactagag gtatgaaaga agaagatatg gcaataatcg
    23821 ctgaggctat agacctatgt ttaacttatg atgaagaatc taaggctaga acattggttg
    23881 ttggtttaac tgaaaaatat cctttatatg aagaatataa tattatggat taattataaa
    23941 gaacaactaa ataaaataca tttaaataat tacaatgatt gtgtatgtat ttgaatatat
    24001 ttagatataa aaaaggtgtc tcaaaatgaa ctttttagtt tatgaggcac ttttttgtat
    24061 ggaatcgaac atatttgtat tatttaaata ataaaaaata tggttatctc tatttttaga
    24121 tggtctcttt gtatttttta attaaaactt taagatttct taagaattat actaggtttt
    24181 tctaaagatt taaagtctat acttataagt atagtaatca tatgaaaaga aaagctttaa
    24241 cgaggaggga atcttatgaa ggtattaatt cttacaggaa aatttggtat gggtcattac
    24301 tcagcatcaa attctttaag tgaagatata aaagcaaaat ttgataattc agaaataata
    24361 attaaagata tatttgagta cattatgcct aactattcag ataaaatgta caagactttt
    24421 tctattcttg taaatcgggg aagtagtctt tataatttgt tttataaatg tgctgaaaat
    24481 ggaaagaaag atataaaatt tactttttca gattattttt taaataaatt agatacatta
    24541 cttcatgaag tacaaccaac agtagttatt tcaacttttc cattttgttc tcaattagta
    24601 tcaagatata aggaaaaata taactcaaat cttcctttaa taacatgtat aactgatata
    24661 agtagtcact cagaatggat tagtaaaaat acagactgtt atttagtagc tagtaaatct
    24721 actaaggaag aacttgtttt taaaggaata gatgaatcta agattaaagt taatggtatt
    24781 ccagtaaaaa aagaatttaa aagaatagaa catgtaaatc attcaactaa aaaaaatatt
    24841 cttataatgg gtggaggatt aggattactg cctaaatcag aacaatttta caaagaatta
    24901 aatagtttag aaggagtaaa aactactgta ataactggta ataataaaaa aatgtactat
    24961 aaactatatg gtagatatga aaatatagag gtagtaggtt acaccaatga agtatataaa
    25021 tatatgaaag attctgacct tattatttct aagccaggtg gaataacact atttgaaaca
    25081 atttattcag aacttccaat attggcattt aatccatttt tacaacaaga gatagataat
    25141 gctagtttta tattaaataa tgaaattggg agaatacttg gtaaaaataa aaaatactat
    25201 gtagatgaga taaaagattt aatttatgat gatgctactc ttaaagaaat gagcagtaac
    25261 atgaaagaat taaaaaaaca atttgataat aataccttag agaatatttt attttcactt
    25321 gatgaacaag gagcgtgtag agaatgtatg tagtaggttt aattatcgtt gttgcattga
    25381 tttttttagt ccattcaatt atacctacat actataataa actattaaat aaagaggtat
    25441 taaaaaatat ggctggagaa aatgaaattg cacttacatt tgatgatggt ccagataaga
    25501 gatatacaga aaacttacta gatgtgttaa aagaaaatga tatacaagct atgttttttg
    25561 tagtagcaaa gaatgctgaa aaagagcctg aaattataaa aagaatgttg agagaaaatc
    25621 atatagttgg tttacattca ttggaacaca gaaatgcatg gctttatagt tatagttatg
    25681 ttaaaaaaga ttttattgaa agtacaaata taatgaaaaa tttaggtgta gatgtaaact
    25741 actatcgtcc accatggggt catactaata tattttcaaa ttcatttgtt aaaaaatata
    25801 atctaaaaat gactttgtgg gatgtaatgg cagaagattg ggagaaagat agtacagtag
    25861 acattattat aaacaagtta atgagtagaa caaaagaaaa ttctataatt tgtttacatg
    25921 atgcaggtga aaattcaggt ggtgcagtag gtgcacctga gcgtactatt gaagctttaa
    25981 agatagctat tccaaaactt aaagccagtg gtttaaagtt cgtaacacca gaaaggatgt
    26041 agttatgata agtaaaactg aaaagaaaaa taaatgccta ggtagtgtag cttttttagt
    26101 tttactaatg ggcataacag gatattttgt ttttagaggg cagtcagttg aatcattgat
    26161 aaaatcatta aaaggtgcaa gccctatgtt tatactaatt ggatttgcaa tgatgtttat
    26221 atatgttgcc tgtgaaggta taaatatata cttaggtatg aaagctttaa accaaaagac
    26281 tactctttta aaatgtatgg gatatgcttt tattggattt tattttagtt ctattacgcc
    26341 ttcagcatca ggtggtcaac cagcacaagt ttattatatg aaaaaagacg atataaatat
    26401 atcatattct tcacttatac tattagtcat tgttgttatt caccaagtag ttatactggc
    26461 atatagtgga ataatgttca ttatggaacg tgagtttata ttaaataatg taagtggtat
    26521 gaacatttta ctaatatatg gtgtaataac aaatgttgct cttgtcattg gagtaattgc
    26581 aattatattt tcaaaaaaac ttgtaaacaa ttttataata tcaataacaa atttattagg
    26641 taagttaaga ataataaaag atgttgaaag ttcaagaaaa gttataatat ctcaaattga
    26701 agagtatgtt aaaggtgctc aatatataaa gcagaatcca aaacttgtag tccaaatttt
    26761 agtcataact attgtacaaa taacatctat gtttttagtt cctttctttg tatacaaagc
    26821 ttttcatcta tcaacatata ctgtatttga gatattagcg atacaatcct tgcttaatat
    26881 tgctgtttct tcattaccat tgccaggggc tgttggagct tctgaaaata gttttatgac
    26941 attatttaag atatttttcc ctggtcactt attagttccg gcaatgttac ttagtagggg
    27001 aataagtttt tatgcatttg tagctataag tggattgata tgtatagtag tacatgtaaa
    27061 atcttctgaa aaagtaaaag cagtgaaaaa agttgcttat gtaagataat attatataga
    27121 ttatttttat taatatgtca atttgtgtgt atattttaat taagctttaa acatgtttac
    27181 aggagtagaa actattaaaa cgtgtgacat tgctaagctt ttgctaataa agtataatat
    27241 tttatatagt ttgaaatatt taagaaatat agcaaaaaaa ggagcttggt gatgaataaa
    27301 gagattccaa aaaaaatttt agtagctgat gataatcctg agattaggga aatcgtagaa
    27361 atacttttaa ctggtgaagg ttatgaagtt attatggcaa cgaatggaga agaagctgta
    27421 aacttagtag atagtacgat tgatttgatt attttggatg ttgtaatgcc tgtaaaaaca
    27481 ggatttgttg catgtaatga aattagagaa aaaacgacag caccaatatt atttttaact
    27541 gcaaaaactc aagactctga taaagttatt ggattttcag caggaggaga tgactattta
    27601 tcaaagcctt tttcatattc tgaactaatt tcaagggtta agtcgctact tagacgttat
    27661 tatatatacc agggaaagga aaaggaaaga gagccaaata tattaaaaac aaaagagtta
    27721 tgtgtcaatt tagatactca agatgttaca ctaaatggag aacctgtgtt tttgacaact
    27781 atagaatata gtatattaat tcttatgttg aaaaatagaa aaaaagtttt ttcgtctgaa
    27841 aatttatatg aaactatttg ggatgagcag tatttttata cagcaaataa tacaataatg
    27901 gttcatatta gaaatttaag aaaaaagtta gaaaaagacc aaaaaaatcc acaatatata
    27961 aaaaccgcat ggggaaaggg gtattatatt gattaacaaa attaagccat ctaagttggg
    28021 agttatacta ttgatatatg tagtaatctc agctgcagtg gctttcattt taaatatacc
    28081 attttactct gctgttgaat ctatagtaaa agatgtatca gaagggaaag tatattttaa
    28141 aagagaagta aatcaactta tggataattt tcaaacttat gtgaataaca atggtataag
    28201 agcagatgat ggagatgata tagcaaaatg gaatagtgat aattggtttg tttctatgaa
    28261 gatatataaa ggaaataagg ttttttatga tacattatac tatcctaatg aagtacctga
    28321 taagagtgag gaaaatgagt tttatagcag tcccatggct agaaactatg agatacaatt
    28381 ttctgatatt aaagctaata tagttgtagt agctttattt agatttagat ttcaaaattt
    28441 tatagaagca atttctatta catttatggc acttacattt attataacat ttttactgtt
    28501 gtttagtaga aagataaagt acctaaggaa aatagaaatg ggtataaaga tagtagagag
    28561 tggcagttta aagtatagaa tcccagtaaa aggtaatgat gaattatcat cacttgcatc
    28621 tagtattaat gagatgagtc atacattgga gaatcaactt ataaaagaaa gggagatgaa
    28681 gaataaaaat aaagatatag taacttcgat atcacatgat gttagaactc cattaacgtc
    28741 agtaatttgt tatttagatt tgattaaaga ccataaatac aatagtcctg agcaattgga
    28801 acaatacata aaaaatgtaa ggtctaaagc atatcaaata aaagatttaa cagatgacct
    28861 ttttacgcat tttgttaatt ctggagagga agtttcaaaa gggtatgaaa aaataaatgc
    28921 taatgaattt atttttcaat tacttttaga tagttcatat acattggagg agaaaggatt
    28981 tcatataaca atagaaaatg aactttataa ccaatatcat atttatgtag atgttacaca
    29041 atttagaagg atatttgata atttatgttc taatataata aaatatgcta ggaaagataa
    29101 gcctatagta tttttaatta aagtagagga ttctaaatta ctaattatac aaaaaaatag
    29161 agtaagtaat ttttctaaga atgtagaaag ttttggaatt ggtataaaga attgtgagaa
    29221 aatagttgca tatcataatg gaagcataaa tgtaaattca gatgataaag aatttgctat
    29281 tgaaatatct attccagcaa aagaatcata aaaatttaaa ttatatattg tgtaattgct
    29341 attatatatt gtgtaattgc ttatgtaaaa ttctatgaag caggaagcat gtgactttag
    29401 ttatgggatg ttcaatattg tgtacctcta ggcaagtaac aagtttactt acaagaccat
    29461 ataatattat tatcttaaaa aaaggggaca taataatatg gttttgacag gagcacatta
    29521 tatttacatt ctatttatgg taattatatt aattactatg attatgaaaa aggatacaat
    29581 agtaccatgt atattaggtg ttttttttat gggattgttc tttgaaaaaa acatctttgg
    29641 tgctataaca gcagtgttta actcttttat tatatcttta aatgagttgg gtccaattat
    29701 cttgattata gctattatgg ttgcattatc taaagcccta gaagccaata atgctataca
    29761 gtatatggtg agaccatttt cgagagttat aaaaaattct aatacagcat tttttgtaac
    29821 tggatttgtt atgttagttc tttcttggtt tttctggcca actccagctg ttgccttagt
    29881 tggagctgta tttttacctg ttgctatgag agctgggcta ccagcaattg gtgttgcagt
    29941 agcattaaat ttatttggtc atggtcttgc cttatctaca gactttgtta ttcaaggagc
    30001 tccaagtata acagctggtg cagcaggtgt tgcagtatca tatgttataa atgatggtat
    30061 gattttgttt tgggtgatgg gaatcgtaac catatctatg gcattttata cattaaaaag
    30121 agatattaac aaaggaatgt ttagagaaga actaaaaggt tttgaaagtg aagaagtaga
    30181 agcgttcaat ggaaaatcta aaatagcaac tattttagta gctttgggat ttcttgctga
    30241 tataattgct atgtatgtat ttgacctaaa aggaggagat gcatcagctc ttttgggtgg
    30301 aacagcagta tttttaataa taattataaa tgttataaat tttggcaaag atagtcttga
    30361 aaatgtatgt gagaatataa ttgatggttt tgtgtttggg ataaaaatat ttggagcaat
    30421 tataccaata gcagcatttt tctatatggg tgaagttgca ccattgacag gtgtatttgg
    30481 aaaagtatta gcaccaggtt cacaaggttt attatcagat ataggcatag ctctttctca
    30541 aacaatacca cttaataaat ttgccgtatc aggtatagaa actgtagtgg gagcaataac
    30601 tgggcttgat ggttctggat tttctgggat ttcattagtt ggttcacttg cctctgtatt
    30661 tggaactgct ataaatgcaa gtgttggtgc attagctgca ttaggacaaa tcagtggtat
    30721 atgggtaggt ggaggctgtc tagttccttg gggtcttatt tcagcagcgg caatttgtgg
    30781 ggttagtcca atagaattgg caaagcgaaa ctttgtccca gttataaccg gattggttgt
    30841 aacaaccata gtagcaatgt ttattattta ataaatcgaa taaatcttta attgtataaa
    30901 ttaaaaatag atgtgtagct ataatttatt ataattgttt tactaattct aagccataca
    30961 tctattttta ttgatatttt tattagtatt ctttaataaa gtaatatttt tcattatatt
    31021 atacataaca tctaaatata acatctaaaa tactgttatc tatatttaat atcttgccaa
    31081 attttttgat gttttattgc cctgtcccac caacatatat aagctatcac aagtttttac
    31141 ctatactatc accaaacaaa aattctaatt ttaatttatt attttataac ctatattata
    31201 ttaactatat tagtttgtag actttagatg taattataaa tattaatact ccaagagttc
    31261 aaattcatat cctagttttc ttgcatttga tataaaatca tttaaaattt ctgtactagt
    31321 tttggaaact gcatgtagta acaagataga accatcatgt agattatcca ttattttttg
    31381 cgttgcttct tcatgtgatg gttgtttatc agtatcccag tctctgtatg caaaactcca
    31441 aaatacagtc ttgtatccta aatttttagt cattgccaag cttttttctg agtattttcc
    31501 cataggaggt ctaaaaaatt taaccatatc tttcccagtt acatctttat ataacttttc
    31561 aacatcatat aattcgtcat taaagttttt tagattgctt gtttttgttg gcatagatgg
    31621 atgggtttta ctatgatttc caactatatg tccctcagaa accattcttt ttactaaatc
    31681 tttattttct tttatatatg gagctgtaac aaaaaaaact gctttaacct ggttttgttt
    31741 tagagtgtcg agtatttttg tagtgtatcc attttcataa ccttcatcaa atgtaagata
    31801 tagtgttttt tgaccatctt ttgtaggtcc attgtatagg gcatcatagt cagaaaattt
    31861 aaattcaatg tccgtattta cttctggtgt tttatgctta ttgtttggta tataaaacca
    31921 atcaagagta gtgttatcta aagaagatac atctaattta ttattatcat tattagtagt
    31981 ggcttttgat ggtttatctg ttccgtctga aactattaca ttggaagtat ctttaccact
    32041 atctattttt tccttatctt cttgtaactg tgtttcctta ttttgatttt cgttttgatt
    32101 gttttgtgaa tttgaacatc caacaataaa aaaaatagaa atagaaagta gtgttaaata
    32161 atgcaatact tttctaaaca ttttgaagct cctttctaaa ttaataataa ttgcatataa
    32221 taataattat atgattaatt acaattgcat atagtattat tataattgga aattactatt
    32281 aataatagtt atatctttaa aaataaagtc acaatagtat gacaaagggt gaataatcaa
    32341 taaatttaaa aaaaaatgaa aaaaatagtt gaccatatta tgagaagatg ttattataat
    32401 aaatgtgctc aacaagaagt aacaaaataa caagcacaaa gaagttaatt gaat
//
LOCUS       DACULQ010000021        31740 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.021, whole genome shotgun sequence.
ACCESSION   DACULQ010000021 DACULQ010000000
VERSION     DACULQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.021"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            573..1229
                     /locus_tag="JA154_04270"
     CDS             573..1229
                     /locus_tag="JA154_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995075.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1737..3008)
                     /locus_tag="JA154_04275"
     CDS             complement(1737..3008)
                     /locus_tag="JA154_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAT7995076.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            3253..3969
                     /locus_tag="JA154_04280"
     CDS             3253..3969
                     /locus_tag="JA154_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HAT7995077.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            4048..5358
                     /locus_tag="JA154_04285"
     CDS             4048..5358
                     /locus_tag="JA154_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HAT7995078.1"
                     /translation="MTELPNEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5458..5994
                     /locus_tag="JA154_04290"
     CDS             5458..5994
                     /locus_tag="JA154_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAT7995079.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            6160..6696
                     /locus_tag="JA154_04295"
     CDS             6160..6696
                     /locus_tag="JA154_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAT7995080.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6706..7461
                     /locus_tag="JA154_04300"
     CDS             6706..7461
                     /locus_tag="JA154_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAT7995081.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            7535..9202
                     /locus_tag="JA154_04305"
     CDS             7535..9202
                     /locus_tag="JA154_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HAT7995082.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            9759..10688
                     /locus_tag="JA154_04310"
     CDS             9759..10688
                     /locus_tag="JA154_04310"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087034.1"
                     /note="catalyzes the formation of glutamate from
                     glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAT7995083.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            10786..11349
                     /locus_tag="JA154_04315"
     CDS             10786..11349
                     /locus_tag="JA154_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAT7995084.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            11837..12673
                     /locus_tag="JA154_04320"
     CDS             11837..12673
                     /locus_tag="JA154_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease IV"
                     /protein_id="HAT7995085.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKKELKMIKEILS
                     SK"
     gene            12957..13952
                     /locus_tag="JA154_04325"
     CDS             12957..13952
                     /locus_tag="JA154_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAT7995086.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(14209..14613)
                     /locus_tag="JA154_04330"
     CDS             complement(14209..14613)
                     /locus_tag="JA154_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAT7995087.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            14774..14964
                     /locus_tag="JA154_04335"
                     /pseudo
     CDS             14774..14964
                     /locus_tag="JA154_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15454..16368
                     /locus_tag="JA154_04340"
     CDS             15454..16368
                     /locus_tag="JA154_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAT7995088.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKNRQQKGYLTG
                     MEEPIIESRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            16474..18711
                     /locus_tag="JA154_04345"
     CDS             16474..18711
                     /locus_tag="JA154_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT7995089.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTKQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            19509..20126
                     /locus_tag="JA154_04350"
     CDS             19509..20126
                     /locus_tag="JA154_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAT7995090.1"
                     /translation="MLDELEKLYPDAKCELNYGTAFELLIATILSAQCTDVRVNKVTS
                     ELFKKYNTARDFANLSIEEISKEIKSCGIYKSKSQKIKDTSEQLCELYDGEVPDSLEK
                     LIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQKTEFALMEAIP
                     KERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            20320..20790
                     /locus_tag="JA154_04355"
     CDS             20320..20790
                     /locus_tag="JA154_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAT7995091.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            20826..21674
                     /locus_tag="JA154_04360"
     CDS             20826..21674
                     /locus_tag="JA154_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAT7995092.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKKLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            21979..22983
                     /locus_tag="JA154_04365"
     CDS             21979..22983
                     /locus_tag="JA154_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT7995093.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            23109..24225
                     /locus_tag="JA154_04370"
                     /pseudo
     CDS             23109..24225
                     /locus_tag="JA154_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
     gene            24715..25476
                     /locus_tag="JA154_04375"
     CDS             24715..25476
                     /locus_tag="JA154_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995094.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            25479..26297
                     /locus_tag="JA154_04380"
     CDS             25479..26297
                     /locus_tag="JA154_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995095.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            26477..27079
                     /locus_tag="JA154_04385"
     CDS             26477..27079
                     /locus_tag="JA154_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HAT7995096.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    27538..27775
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            27839..29758
                     /locus_tag="JA154_04390"
     CDS             27839..29758
                     /locus_tag="JA154_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAT7995097.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            30526..31617
                     /locus_tag="JA154_04395"
     CDS             30526..31617
                     /locus_tag="JA154_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995098.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT        12718 a         2998 c         5882 g        10142 t
ORIGIN      
        1 tagtagataa gaataccaga aaaaaataga tagttaaagg gttagggtaa aagagtaatt
       61 gtaaaataaa tatatgggtg atggtggata agaatatcag aaaaaataga tagttaaagg
      121 gttagagtga aagagtaatc gtaaaataaa tatatggatg atgtggataa gaatagtaag
      181 aaaaaataga tagttaaagg gttagggtga aagagtaatc gtaaaataaa tatatggatg
      241 atggtagata agaatatcag aaaaaataga taattaaagg gttagggtga aagagtaatc
      301 gtaaaataaa tatatggatg atggtagata agaatatcag aaaaaataga taattaaagg
      361 gttagggtga aagagtaatc gtaaaataaa tatatagata atggtggata gagtagtaag
      421 aaaataaata gataaaggga tagaataaat ttaaatatgg aaaatgaata tctaataata
      481 agtgattaat aaataattgt agatagattg taaattaaat aaatctttaa tagaaaggag
      541 aactatttat atattaaata gtatttataa atatgaaaaa aggcgatttt atatggagtg
      601 gagttcttat ttgtattatt gctgtcttag caataccaag tagtagagaa gtatttatgg
      661 aagcaacagg tgcacatcca tatattggag gttttttaaa gtttgctgta ttagctacaa
      721 tgggagattt acttggtgct agaattttgt ataaggattg gaaaatacca gtaggagtat
      781 tttatagagc aatagtttgg ggaataatag gtgtagttac cacatatgga atgtcaatct
      841 actcatcagg tgtaggtcaa gcacaagtgg atggtatgct tccatttgag ggaatagctt
      901 ttgcaaatgc tctatttaag agtgcaacta tgaatatact aatgtcacca actgtatttt
      961 tattccataa ggttatggac tgttttatag ataaaaaata tgaggttgga aaggggatta
     1021 aggtaaaact taaagatgtg gtaaatattg tggactggaa tgcttacctt ggttttagtg
     1081 ttttaaaaac tataccattt ttctggatac catgtcatac aattgtattc ttattccctg
     1141 ctgagtatag agtaattgct tctgcatttg catctatagc tttaggtcta atattggctt
     1201 tagctaataa gtctaaagtt gcagtttagg ttcactgatg aagatagtag tattatttaa
     1261 attaggaata actttttaaa ttaatattat ttgtatttat aataaataat aatttatata
     1321 agaagtaata ttctaagtga tggatagtgt tttaaataat aagaagtaat actctaagtg
     1381 atagatagtg ttttaaataa taaggagtaa tactctaagt gagaagtagc attttaaata
     1441 ataagtgaga ttttaaataa aaagtaggtt tttcgttaga atagcatttt attaaactta
     1501 aaaataaata taatatttta aagaaatcta agttgctaag cactgttttt aagaagctta
     1561 aaatccccca tttaaagttt taagattcta aaaaaacaag ttataactaa aaaatggctg
     1621 ttaagactac aatacttata agtaatactt atggtatata ttagttcata acagccattt
     1681 ttattttaaa ttaataattt tattcaataa tttgagttta tgttctaaat caagctttaa
     1741 attaaaggat ttaaagaagc tattctagtt gtagctacat aaactctgga tatttcatac
     1801 catgttttaa aatctactac tccgctttga ggtaaaccaa agattccttg gaatatctta
     1861 acagcgtttt ctgtatctgt tccatagata ccatcttcta taacttttgg tacagctgga
     1921 taagaattag atatagcatt taactgattt tgtatggttc taacatactg tccagaagaa
     1981 ccaacttgta gtgtagtacc agggaatgac actggtacac cacttactat aggcgctctt
     2041 tcaaatacta tctcatctcc atagaaatat cttagtatac tctcatagtc atatccttgg
     2101 tcacctaagt ctttactgcc ccattgagtc atttggtcag gacattggga tttttgtcca
     2161 tcacaatatt gtgctaatag tggctgtctt gatgtaggag gtctttttat aaaagtatta
     2221 aaaatagcat ctacaactac gttaattggt tcaaataagt ttctattatt tataaactta
     2281 tggtcgtatg ctgttgttga agttattgta aagttatatc ctttatttct ataccattca
     2341 gtaaagactc tatttaaagt aaatgatatt atagctatta catttgcata aattgtctgt
     2401 tcaggccaag ttgaatatat ttcagatgat gctatgtttt taatgtactc tttaaatgga
     2461 atccaatagt ttggagctga tgaatcttct ggcaaaccat catgaactac tataaattca
     2521 ggcactacag ggttatctaa tacaacgaaa ccagtaggag gtggtaatgg ttttaagtta
     2581 ctttctggaa tcttaggagg ataagtacca tatagagtat gttctcctat gtcaaaaatc
     2641 aactcacttt gtcttgagaa agaacgctta cttcttgttc taggagacat gggaactcct
     2701 tgtcttgctt caattgtagc taacaactgt gttccttcta tgacaactgt ctcatatcca
     2761 tctgctatgg cttccactat atactggcta taaggtctaa catcagatgg ttgtagagag
     2821 taatctacat ctggtgctgg taatactaaa ccagtcactt gaccagattc attacttctt
     2881 aggttttggt ataaagttga tgagctttgg ctaccattag acattgaata tatgtttact
     2941 actgcatttt gaataggtct attgttagta gcatcaatta tgcttactgt taagaaacca
     3001 tcttgcatca aatcaccctt tctttaaatg aatttagttc ccttatattt caagatatga
     3061 atatagctta aaatttgtta aataatatag atataaattt aaattttttt gagtatttga
     3121 gtatatttgt atttaaataa tttaaaataa tttaatgtat attttagtat gtatttaaaa
     3181 cacgaataag ttaaaaataa gtagatgata taaatcataa ataaatcaaa aattttgaaa
     3241 ggaaaataat ctatgaagct agatttattt ttattagcaa tagttcctat attgatagga
     3301 atgttttgga taaggtctaa agatagatat tgtagagaac ccctaatcca tctaattaaa
     3361 ttttttttaa taggtgcttt tctaagtgtt ataattatac ttttagaaaa tttactgatg
     3421 aagtttaatg tatttgaagg gtattcagaa ctaatatatg tatcatttgt agttgcagga
     3481 cttgtagaag aaggtgtaaa agctctgata ctgattcctg ccctaattaa agaaaaacac
     3541 tttactgaaa agttagatgg tattatttac tctgtatttt tagctttagg atttgcaaca
     3601 attgaaaata tggtttatat tttttctgaa agtagaaatc tagcactgca agttggtatt
     3661 aatagggcag ttatctctat accagctcat gtaatgtttg caattactat gggctactat
     3721 atttctaagt ataaatttga gggaaataaa aataaaagac gtgagtattt gtttatggca
     3781 gtgttaattc caattttatt acatggagtt tttgatttca tattgatgat agaatataga
     3841 tgggctatta tattacttat agtttatgta ataattttgt ggaagataaa tcttgataaa
     3901 cttgagaaat atatgaatca ctctaaaaaa gtgtttttcg gtaatcttag gaaaaagaag
     3961 aagaaataaa atttaaagga aagacataaa taattaaagt ataataaatg acacacaagt
     4021 aaagcagatt gaagggggat tatagatttg acagaattac caaatgaatt tttagaagat
     4081 attgaagaaa tactaaaaga tgaatatgat gattttataa aaagttatga ggaaagtaag
     4141 actactgggc ttagaataaa tactttaaaa attgacaaga aaaaattatt tgatttggag
     4201 ttgtttgatt tgaatcaaat accatgggca gaagaaggtt tttattatga tgataaaatt
     4261 gacagacctg gtagaaatcc actgcatgag gctggagcat actatttgca agagccttct
     4321 gcaatgagtg taataccaaa ggcagatatt aaagagggtg aaaaagttct cgatatgtgt
     4381 gctgctcctg gtggaaaatc tacatatata ctatctaagc taaacgatac aggtttattg
     4441 gtttctaatg aaattaatgc tagtagaatt aaggctctag gggaaaattt agagcgattt
     4501 ggagcgagaa attgtattat aacaaacacc gattctaata gcttaagaaa gacgtttaat
     4561 gggtattttg ataaggttat tatagatgca ccttgttctg ggcaaggtat gtttaggaag
     4621 gatgaggttg ccatatcaga ttggagttat gcaaaggtag tagaatgtca atccatccaa
     4681 aaagagatta taagagatgg atataaaatg ttaaagaagg atggcatttt aatttactca
     4741 acttgtactt tttcaaggga ggaaaatgag catgttataa atgagtttat cagtgaatat
     4801 ccacatgcag aacttattga aatggaaaga ttatggcctc ataagataaa gggagaaggt
     4861 cattttgttg ccaaaataaa aaaattagaa gaagaagaat gtaatgtcaa agaattaaaa
     4921 ataaaaaatt tagataaaga aataaaggat tatagggagt ttgaaaagaa atttttaaag
     4981 gttgattttt atagtaataa aaaaaataaa ttttatttaa gaggagaaaa tttgtaccta
     5041 cttcctgaaa tatatccaga tacgaaaaaa ctaaaagtat tgagatatgg tttgcattta
     5101 ggggttttaa agaagaatag atttgagcct tctcatgcac tatctcatta cttaaaagtt
     5161 gaagatgtaa aaaatgttga aaacttcagt gtacagagtg aatcaattct taaatattta
     5221 aaaggggatg ttgtaaattc taatgatagt agaggatggg ttttggtttc ggttgaagga
     5281 atacctttag gttggggaaa agaatcttct ggtgtaatta agaatcatta tcctaaaggt
     5341 cttagaaaag tattttaaac tattagaaaa aagaagtata atatttatat tgtaggttta
     5401 tggtagatta ttttgctgta gattgactta atatttaaca cagaggagac gattttagtg
     5461 ggtgaagcag ttaaaaaaga agttgtagaa tggataaaag tgattgtcat agctcttgtt
     5521 ttggcatttg caataactcg ttttatagtg ccaacaatag tcaaaggaga atcaatgtat
     5581 cctacattag ttgaacgtga ttatttgata gttaacagaa ttgcgtacaa ggtaggagag
     5641 ccaaaataca aagatataat agtattcaaa accgacttaa cagaggaaaa tggaaagaaa
     5701 aaagatttag taaaaagagt tataggtgtt cctggtgacc atgtaaaaat acaagactcc
     5761 aaggtatatg taaatgataa gttgttagat gagacttcct atatacataa taatcgtact
     5821 gatggagata ttgatatcgt agttccagaa ggaaaattat ttgcaatggg agataataga
     5881 gaaaaaagtt tagatagtag atacgatgag gttggattgg tcgacgagca taccatttta
     5941 ggaaaggttc tagtcagatt gtatccattt tctaagatag gaactattga ctaaacatat
     6001 aaaatttgga gcaatcgctg attagctgaa atcaccagac catacacaat agttgtctat
     6061 ataaaaaaga caacacctta tttagtagac ttatctaaaa gatagtgttt aagagacatt
     6121 atcaataaag ataatataac tataaaggag aatttgtgaa tgaatgaaac tattaaagaa
     6181 gagattgtag agtggataaa aataattatt actgcacttt tttttgcatt tattataact
     6241 cgttttataa aaccaacatt agtaaatgga gaatcaatgt acccaacact taaatcacat
     6301 gattatttgg tagcaaacag gatgacatat aagttatcag aaccaaaatg tggagatata
     6361 atgatattta agactgattt attacaagag aatggaagga aaaaagagct tgtaaaaagg
     6421 gttataggtg ttcctggtga ccatctaaaa attaaggatt ctaaggttta tataaatggt
     6481 aagttattaa atgaagtttc atatatacat gataattata ctgaaggcga tattgatatg
     6541 gtgattccta agggaaaagt atttgcgatg ggagacaata gagaagttag tttagacagt
     6601 agatataaag aagtgggatt agtagatgaa gaaaatatta aaggaaaagt tattttaaga
     6661 gtatttcctt ttacagatat aggtattttt gagtaggtga tttgtatggc agttggtata
     6721 gcgtccttat tgtttaatat tgaggaggct ttaaacattt gtgagagtat aaagcaaata
     6781 actcatttag agattggaat tgacaatata tctgaatgta gtgagttatg taaatataaa
     6841 gagagaattt ctaagttagg attatctata gggatacact tgcctatgga attaaatact
     6901 tgcgaaaaca tagagtatat acgaaattca tggataaatt ttattgagaa gatagaattt
     6961 gaactaaaag gatttgattt aaggtatttt aaccttcatt tgggatatgt tatgacaaat
     7021 agagttagaa agaatagaga caaatattta ggaaatagtg tggatttttt agataaatta
     7081 aatacaaatt catatgtatg tatagagaat acttattcaa aaggtgggga tttttcaaat
     7141 attggaaata tatcttatga ttttgaatat atatttaaga ggattaaaaa ttctaagatt
     7201 tgtttttgtt acgatactgg acactgttta attgatgagg atgcttatgt aaagaactta
     7261 aaggataaaa taagactgat acacttaagt gataatgatg gtatcaatga tacacatgtt
     7321 ggtataggaa gaggaatttt atctgaagag gggattaaag aggtcttaac attagatgca
     7381 gaatatttag tattagaaat aaattacgaa gacattgaag atactataag taaattgaat
     7441 aatattgtag gagagggtta aatacctctc tttatcttgt tgatatgcct cattaattaa
     7501 aatttatatt catcaaaagg aaggagatgt tgaagtgaaa aaaataaact taaaaataga
     7561 aggaaaagat aaggaatatt cattagaaga aaattcccct ggaatcagat tgggagatat
     7621 agcaaaagaa ttttgtgatg agcataaggg ttatataaca cttgcagttg tagacaataa
     7681 actaaaagag ctaaattgta gagttaagaa ggattgtgag ataaattttt tagatactac
     7741 taatgaagat ggagagagag tttactttag agtcatgtca tttatatttg taatggcatg
     7801 tagagaaatc ttttgggata gcagagttac aatagaacat tcactatcag atgggctata
     7861 ttgtgaagtt catatagata gaaagcttaa agaagcagat gttgaaaata taaaaaacaa
     7921 gatgaaagag atagtaaaca acgattatgt tatagaaaaa attgaagtta caagaaatga
     7981 agctatttct atctttgaaa ataatgaaat gtatgaaaag gcagagcttt tgaaatacaa
     8041 agagtatgat agtgctaaag tatataagtg tagaaactat atagaccatt tttatggtta
     8101 tatgttgcca tcaactggtt atataaaatc atttgatatt aagctttata atggaggcgt
     8161 tataatttta ggacctagtg aagaagataa gactcttcct atgaaatttg tacctcaacc
     8221 taagctatca agtgtatatt atgaagcaga agaatggtca agacttatgg gaattgacaa
     8281 ggtagtatct cttaataaaa taatagaaaa caatgaatat ggagatataa taaggacatt
     8341 tgaagcattg catgaaaaga agctatctca aatagcggat atgataaagg atggcaataa
     8401 gagagtagtt ttaatagcag ctccatcttc atctggaaaa acaagttttg ctcacagatt
     8461 atcaatacat cttagagtaa ataatttaaa tcctatttca atttctttag atgattattt
     8521 tatagataga aaacatactc ctttagatga atatggaaat tatgattttg aatcaattta
     8581 tgcaatagat ttagagaagt ttaacttaga cttaaagaaa cttttagctg gtgaagaaat
     8641 tgatgatata agattcaatt ttaaagaagg taagagagaa tacactggta aaaagataaa
     8701 gatagattca aatcagccaa ttatattaga aggtatacat gggctaaatc ctattttaac
     8761 atcatctata ccagatgaag ataaatttaa gatatacata agtgccctta ctcaaataaa
     8821 tttggatgac cataatcgaa tccctactgc tgatttgaga atgataagaa gaattgttag
     8881 agactataat tttagaggat atagtgctga taatactatt ttacaatggg catcagttag
     8941 aagaggagaa aagaagaata tattcccgta ccaagaggag gcagatgcta tatttaactc
     9001 tgcttgtgta tatgaattgg cagttttaaa acgatttgcg aaacctttgt tggaagagat
     9061 aaaggaagat aatcctgcac atattgaagc tactagatta ctaaagtttt tgcagtattt
     9121 tgttgaattg gacgatactt ctgacatacc aggtatttca attcttaggg aatttattgg
     9181 aggaagtaaa atagttgatt aatgtgtgta ttatagattg ttttatgaag attaaatggc
     9241 atagcaagta gtgttattgg tttatgttaa gtatattaag tgtaaagtgg agaattaaat
     9301 tgatacagat tttaaagttt gagttgatat ataaaaaggc ttgttttata cttttgtatg
     9361 aagcaagctt tttattttaa attttacttt tttgtttaat attttgttag tttatgtgat
     9421 tattttttaa aagagtaaaa gagtaaaaga gtaaaagagt aaaagagtaa aagagtaaaa
     9481 gagtaaaaga gtaaaagagt aaaaggtgtt gggttagaaa tttttggtgt tataaaaatt
     9541 taaattactg gattattaga ttgctggatt gctaaattac taggttgtta aattattgaa
     9601 ttactaaatt attaagctct ttaaactatt taaattgttt gaaaaatgaa ttgattcttg
     9661 tagaaaataa ttttaaagta taattttttt taacattgtt ttattatagt ataaacttgt
     9721 gctaaaatag aatatataac ataatataag aggtgcatat gaatacgtta gatgagacat
     9781 tattaaaaga aattataagt tctaataaga agtatactaa ttatggacaa gtcgctagtt
     9841 acatcccaga attaaaaaac gctagaagaa atgatttagg aatttgtatc atagatagcg
     9901 aaaataatct ttatagtgct ggaaactgta gcacaaagtt tactatacaa agtatttcaa
     9961 aaccaattgt gcttgctatg gcacttatgg ataatgattg ggaagacgtt ttttcaaacg
    10021 ttggaatgga gccaagtggg gacccattta attcaataat gaaacttgaa attaatgaca
    10081 caaaaaaacc ctgtaatcca atgataaatg caggggctat agtaaccact tcccttataa
    10141 atgggagttg tttagaagaa aaagaagaaa gaatgcttag tttctttaga aagttagcaa
    10201 aaaatgataa tataggaata aactatgatg tctataagtc tgaaaaaatg actggagata
    10261 gaaacagagc tatggcttac ttattgaaaa gtgatggatt tatcagaggg aatgtagaag
    10321 atgttctaga cctttatttt aaacaatgct caattgagat agattctgta gatttagcga
    10381 gaattggaat aaatcttgca aattatggag tagatataga aaatggtgag catttaatga
    10441 gtgaaatggt ttctaggatt gtcaagacat ttatgatgac ttgtggaatg tatgatgctt
    10501 ctggggaatt tgctattaaa gttggcattc ctgcaaaatc tggagttggg ggtggtataa
    10561 tggcttctgt gccgggacgc atggggattg gtgtttatgg acctgcgctt gacaaaaaag
    10621 gtaacagcgt cgcaggagtt aaagttttag aggagttgtc aaataaactc aaattaaata
    10681 ttttttaaaa tattaagaaa ggattttttt gacaacaaca tataatttaa tatataatta
    10741 aattgtaaag aaatatgaat taaataaatt ttttaggagg ggtcaatgaa agaggtattg
    10801 gtattacgag atttggagtg cataaaggct attgcgcatc cacgtagaat agatattcta
    10861 aaagcatttg acaaatcgcc attgtcagca aaacaattat ctcaattatt ggaagaacct
    10921 catgcaaaaa taaactacca tataaaaatg ttatacaaag ttggtatttt agaattagtt
    10981 gaggaaaaaa taaagtcggg gattgtggaa aagtactatt atccaagtgc aaaaaatgtg
    11041 gttataggga ataggatact taatttttct ttagacaatg gggaagaaaa agaagaattg
    11101 tacatatcaa aatttgaaaa tatgagtgaa gttttttata aggcagcaga ggaagatgtt
    11161 ttagaaaatg aaaatatagt tgattatcat gatatttctc ttactcatga tgaattagtt
    11221 gaacttagtg acactatgaa gtcaaagata gatgaaattt taaataaaag acaacacaat
    11281 gttgaaggtt ctaagtatga tatagctatg gttatagttc ctactcttga ggaggaatgt
    11341 cctagttaga gaattaatta tggattaatt aaagatataa tattagagat tgaacatcaa
    11401 aatttactta ataatcaaaa aatagttttt ggtatgggta aaacaatgat ttttaatctc
    11461 tatttttttg tttttttata cctattgtat ttgtaaataa tatgtatagt gtaagatgta
    11521 tacactatct aagaagtgct tagataatat acaaagtatg taagattata atatttgaaa
    11581 ttgattaaaa tgggtataat taaagtgtaa atattaaaat taaaaattaa tataaattaa
    11641 atcaatgcta gaaatatgtt aaaagaggac tgagattagt tgggatagaa catcccttag
    11701 agcttacgaa tttttagtga aaaagtacta tcaaatagga atctccatct tcatatgttg
    11761 gctaaatgta gttaaggtgg agtagttcaa aattaaaatt gagtacaagt agtagtacaa
    11821 ataaaaggag attaatatga ttaaattagg aacacatctt tcaatagcaa aaggatttgc
    11881 taatgcggct gagacagctg tatatattgg tgcaaataca tttcaatttt ttagtagaaa
    11941 tccaagagga ggaaatgcta aagaatttga tgaaaaagat ataattaaat ttcaagaaat
    12001 tagaaaagaa aataattttg caccactgct tgcacatgca ccatatacta tgaatctggg
    12061 tggtacaaag gatgatgtat acgaatttgc aacaagggtg atagatgagg atataagaag
    12121 gatggactca ttaggaattg aatacatgtg ttttcatcct ggaagtcatg taggtggtgg
    12181 tgtagatttt ggcatagata gaatagtaaa aggattgaac aatgccataa agggtgatga
    12241 aaatataact atacttcttg aaactatgtc gggaaaggga actgaaatag gttttaaatt
    12301 tgagcaatta aaaagtataa tagacaaaat tgagcataaa gaaaaaatag gtgtgtgtct
    12361 tgatacatgt catatttttt cagctggtta tgatatagtg aatgatttag atggggtttt
    12421 ggaagaattt gataaggtta taggcataga aagattgaaa actgtccatc taaatgatag
    12481 tatgatgcca tttggagata aaaaagacag acatgctcct ataggggaag gaaagatagg
    12541 attaaaagca ttaattgatt ttatggaaca tcctagtcta aaacatttac catttttctt
    12601 agaaactcct tttgatgatg aaggtcacaa gaaagagctt aaaatgataa aagaaatact
    12661 atctagtaaa taaaccttta ttaaaaatat gtgaaatact tcaccctcaa cataattagg
    12721 gtggagatac aatcatgaaa tgtgataatt taaattaatg gataggaata acaaaaattg
    12781 tatacaaatt aactgggtat aaagtatgat tatattatga atagactgat ataaaatata
    12841 attatactat gaatagtatg taaatatatt gcaaagattc tcataatttg ttgtataatt
    12901 aaagatgaca tatttatatt atataaaaaa ttattaaaag ttaggtgggg aaagatatga
    12961 gaaatttagg caatactaac atgaaaataa agagagttgg ctttgggggc attccaatac
    13021 aaaggattac tcaagatgat actaacttag ttataaatga gttagaaaaa caaggaataa
    13081 actttattga tagtgcaaga gggtatacaa taagtgagga agcaatcgga attgccattg
    13141 aaggaaaaag agataaattt ttcttagcta ctaaaagtat gtccagagat tatgattcaa
    13201 tgaagagaga tgttgaaata agtttaaata attttaaaac tgattttata gatttatatc
    13261 aattccataa tgttaaagaa gaggagtatg ataatctttt taaagataaa atggcttata
    13321 gtgccctttt agaggcaaag gagcaaggta agataaaaca tattggtata actagtcaca
    13381 atttaaatac tatagaaaaa gctatagaag atggaaaatt tgacactata caattccctt
    13441 acaatatagt tgaaggacaa gcagatgaag tgtttaaaaa agctcatgaa aaaggaattg
    13501 ggataatagt aatgaaacct ttagctggag gtgctttaga caatgcaaca ctggctatta
    13561 aatatatact atctaaagat tacatagatg tagttatacc aggtatggag agtgttgaac
    13621 aggttagaca aaatgtcgct gtacttgaaa atcttgtgct agatgaaaaa gataataagg
    13681 aaattgaaga aataagaaat tctttaggta aaaaattctg tagaagatgc gaatattgta
    13741 tgccttgtgc tgtaggaata aatataccat tgagtttttt atgtgaaggt tattatacta
    13801 gatatggatt aaaagagtgg gctaaagaaa aatatgaggt tatggatgtt aaacctactg
    13861 agtgtattga ctgtgggctt tgcgagagta gatgtccata tgaacttcct ataagagaaa
    13921 tgcttaagac tgttgttgaa aagctaggat agattttatt tattgaaatc ggtgtattaa
    13981 atttactccg atttcttttt attagatgtg gtcttatata caaatattaa atttacatag
    14041 ttatagctat tatataaatt taatatttgt ttaatactta ttaaatattt acttaatgct
    14101 ttttaaaaac atttgtttga gtatagacat aacacaatta ttattgttgt attagtatag
    14161 tttcaaatac tggagtatca atataatgtc gaataacaca taaaatcttt atttttcaaa
    14221 aatattatag gatatatcgt atttagatac tggataaagt tcaatttcaa ccctagggtt
    14281 atctttatct ataaaatcaa ataaaagtac tggcttaagt tgtttatcat tttttattat
    14341 tccacttttt tcaataccat cacaaatact ttttggataa ttatgtaaat cccccattct
    14401 cttatttggg aagaataatt tcaagactgt tattagattt tcttcaacag tttccccttg
    14461 atactgttgg tttataaaac ctgcaatcat gttttcgtat gcaagatact tagaatattt
    14521 tccctttgaa ggcatccatg cttgaccatt tatgttatga agcttaaaat tagatttact
    14581 aataggtcta cctggaattg ttattttaat caaatttttc accatcctta tacagtattt
    14641 attataatct gtatatgatt taaaagcaaa ttgttattta aagtgttttt acagagcttg
    14701 tttgtaaaat tttccacttt agatgtagta atataagata agtattttgc ttacaaattt
    14761 taaaatttga tatatgatgg gcatattcat attagactat tataagacct tcataatatt
    14821 aataagtata agtttagtaa agagagtaaa aagaaatcca tataagtcaa gtaaagactt
    14881 aataaatggc aatgtaaatg ggatattaat tataagaaaa aatcaatctt tttgcttaac
    14941 tatagaaaga atgtcaaaat ataactaatg atatttgaag ttttagagtt tatattaaaa
    15001 ggtaagaagg ttaagtgggt aaaaaaggtt gaatagatta aaaaggctaa gtatctaaaa
    15061 gagttaagta ggtaaaaaag ttaaataagt aaatgaaaaa ggctaagtat ctaaaagagt
    15121 tagtaggtaa aaaagttaaa taagtaaata aaaaagatga agtatctaaa agagttagta
    15181 ggtaaaaaac taaataggta aaaagatgaa gtatctaaaa gagttagtag gtaaaaaatt
    15241 aaataggtaa aaagacgaag tacctaaaag agttagtagg taaaaaaatt aaatagataa
    15301 aaagacgaag tatctaaaag agttaagcag gtaagaaaat tgaatagcta aaaagattaa
    15361 gtatttaaaa gagttagtag ctagaaaagg ttaaaaagac taaaaaaatt aaataaattt
    15421 aaaaagtcaa ttagattaaa aaggaataat tttatggata ataaggaatt aaaagcattt
    15481 tgtaatttaa taggattaaa ctgtgtagga gtagcagggg tagataaata tgataattta
    15541 gaaaagatac ttaagaacag acaacaaaaa ggttatttaa ctggtatgga agaaccaata
    15601 attgagagca gaattaaccc aagacatata atgaaggatg ctaactctat aatagtctgt
    15661 gcatttccat atcatataaa tagacaagaa aagaataaaa attcaaattt atcaaaatat
    15721 tgtcatggaa aggattatca cattgtagta aaggactttt tacaacaaat ttgtgattat
    15781 atttctaaaa cagtaacaga ttttaaatac aagttatttg ctgataatgg accattagtt
    15841 gacaggtatt tagcttattt atcaggaata ggatattttg gaataaataa taatatcata
    15901 actgatgaat atggttctta cgtatttata gggtatatag taaataatta taaatttgaa
    15961 gaagataaac cactagaaaa aagttgtttt aaatgtggta agtgtgtaaa atattgtccg
    16021 ggaaatgcta tattgggaaa ttatgatatg aaccccaaaa aatgtttatc atatataact
    16081 caaaaaaaag gagatttgtc agagaaagaa aaagttgttt taaagaatgg caaaaaggta
    16141 tttggatgcg atatttgtca agaagtatgt ccacataatg ttgaaatacc aacaactcat
    16201 attttggaat ttaaagaaaa tataatagat tatttggatt atgatgagat acaaaatatt
    16261 tcaaataaag aatttaaaag gagatatgga gatagagctt ttagctggag aggtagaaat
    16321 ataataaaga gaaatatgga aataatttta gataagtatg atgactaata ataagagtct
    16381 gtttcatata ctttaatgtg tatttacttt aacacttgtt gtgtaaaagt catattatat
    16441 cattaaagtt aaatatgggg aggtggggga atattgaaat ataataatac atctgaaata
    16501 aatccactag attatatatt gggtcaagaa agagctgtag aagctatgga attagggttg
    16561 aaaataaata atccagcgta caacatatat atagctggtg aacctggaac tggtaaaagt
    16621 acttatgcct taaaggtttt aaatgagtat gcatccaaaa aaaatacgca taaagattgg
    16681 tgttatatat ataattttga aaaccctaga gagcctatta tagttgaatt agaaaagggg
    16741 tttggaagag aacttaagaa agatatggag aaattaattg aaagtctttt agaagatttt
    16801 aaaaatgcgt ttgaaagtga aaattttgaa atagaaaaaa ataaacttct ggatgaatat
    16861 gaaatagaaa aagatatgct tttgaagcaa attaaaaaat atggagaaga aaaaggattt
    16921 aaattaaaac agagtaaaat gggaatagtt tttatccctt tgaaagaaga ggaagatgaa
    16981 agtgatgagt ctgatgaaga gttttacaaa gctaaaagag aactagaaaa tatggcgata
    17041 caggtcgtat ataaaattag aaacttagaa gaaatagctg aaaaagcagt gttagagctt
    17101 gaagaagaga tagctaaact tgtagtagaa cctcatatca agagattgtg tgaaaaatat
    17161 gaaaattatg ataagattca aacttatctg gaaaatataa agaaagatat aatagaatat
    17221 atgtatttat tctatttaga tgaagaggag ttaaaagata agtatgataa agagcatttt
    17281 attaaatata aaattaacct atttgtagac aatggaagct caaagaataa agaatcagca
    17341 cctgttgtag tagagataaa cccaagtcca gcaaatctat ttggaaaggc agagtatgat
    17401 tatgcaaatg gaaatataaa gactgatttc acaaaactat tatcaggagc atttcatagg
    17461 gctaatggtg gttatttagt actttatgca gaccaattac taaaatatac aatgtcttgg
    17521 gaaattctta aaaagacttt gcagactaaa aaagtaatat tggaaacaca aacagcaatt
    17581 aaaccagaaa atatgccatt ggatgttaaa ttagtactga ttggaagcca ctatatatat
    17641 gatattttat atagatatga tgaagatttt gaaaaatatt ttaaagtatt tgtagacttt
    17701 gatagtgaaa tggataaaaa tgaagataat gaagaaggaa tagcaagatt tatagcatac
    17761 caatgtgata agaataattt aaggcatttt actaaatcag cagttgaaga ggttattaaa
    17821 tttagcacta gattatctgg tgatttagaa aaattatcaa ctaagtttaa taaaataatg
    17881 gaagttgtaa tagaaggaag tgcttatgca gagtttagaa attcggagta tacaaagcag
    17941 tgtgatgtta aaaaagcaat aagtgaaaag agaaaaagaa tcaatagagt ggaaacacat
    18001 atggatgaat caattgaaaa tggatttact ctaatagaaa cagagggaag aaaagtagga
    18061 gtgataaatg gtttgtcagt cttaagtaca ggagaatact cttttggtag agcttcaagg
    18121 attacagcga ccactagccc aggaagtaaa ggcatagtaa atatagaaag agaagtaaat
    18181 atgagtggct ctatacataa caaaggagta cttatattag gtggctatct ttctgagaat
    18241 tttgcacagg atttactatt gtcactaaat gcatatgttt gttttgaaca aaattatggt
    18301 ggaatagatg gagatagtgc atcttctgca gaattatatg tactattatc atctcttagt
    18361 ggagtatcaa ttaaacaaaa tattgctgtt acaggttcta taaatcaaaa gggtgatata
    18421 caagtagttg gtggtataag tgaaaagata gaaggatttt attctatatg taaaactaaa
    18481 gggttaaatg gtgagcaagg cgtcataatt cctagaaaaa atcaaagaaa tctcgtttta
    18541 tcagatgaag ttaatgatgc agtgcgagat ggaaaattta agatatatac tgttgaaaga
    18601 gtagaagagg ctatagagat attgacagat gttaaatttg aagaaataaa gaaattggta
    18661 aaagaaaaat taatatcttt cagcaagata caaactgtgt caaaagaata agtttattat
    18721 agaatttaat gggtatttat gtataataaa aagcaagaac tttaataggt ttttgctttt
    18781 tttgtaaata tatataaatt aaattcttat tttgtatgtt ttatatattg atgtttattt
    18841 ttttgttgat atgattaaac tatttataaa tagcgtttta taaattggta ttttgaaggg
    18901 ggataattat gaaaaatgta aatctaaaag agataaatga aaaagatgta attttggaag
    18961 aagagataaa taaaaaagga atggataaga aaaatataat tgagaaggaa gagataaata
    19021 aaaaaggaat agataagaaa gatataattg agaaggaaga gataaataaa aaaggaatag
    19081 ataagaaaga tataattgag aagaaagaga taaataaaaa aggaatagat aagaaagata
    19141 taattgagaa gaaagagata aataaaaaag aaatggataa gaaagatata attgagaagg
    19201 aagagataaa taaaaaagga atgaataaga aagatataat tgagaaggaa gagataaatg
    19261 aaaaagaaat agataaaatg attggtaaaa aagatgagat aattgatgag aaagatatag
    19321 agaattttat ggatattgaa gaagataaaa aaagtatcaa agtaagtaaa aaaagtacta
    19381 aaaaaggtga aacggagaaa aaaacaaaga agagtagttc taataaggct acacaaaaaa
    19441 caagtaaaga aactaaaagt aagtttaaaa ttaaaaatga gtcagcaaat gaaaaagacg
    19501 taaataagat tttagacgag ttagaaaaac tgtacccaga tgctaaatgt gagttaaatt
    19561 atgggacagc ctttgaactt ttaatagcaa ctattttatc agcacaatgt actgatgtaa
    19621 gagtgaataa agttacttca gagttgttta aaaagtacaa cacagctaga gactttgcta
    19681 atttgagcat tgaagaaatt agtaaagaga taaagagttg tggtatttac aaaagcaagt
    19741 ctcaaaaaat aaaggataca tctgaacagc tatgtgaatt atatgatggt gaagtacctg
    19801 atagcttaga aaagcttata aaattacctg gtgttggaag aaaaacggca ggggttgtac
    19861 ttagtaacgc ttttaatcat ccagcaatag ctgtagatac tcatgtattt agagttagca
    19921 atagaatagg aatagtggac gagccaaatc ctcaaaaaac agaatttgca cttatggaag
    19981 ctataccaaa agaaagatgg tcacactcac atcatgtatt aatttttcat ggaagaagaa
    20041 tgtgtaaagc tagaaatcca gaatgtgcaa gttgcccaat aaaagaagat tgtaattatt
    20101 ataaagaact taatgaaacg aaataacttt tagaaagtaa gttgtacaag agggattttc
    20161 tgatataata atacttgtga aaatgttaga taaaaaacta ttatatacat gaaataaaaa
    20221 ttattattaa cattatttta tataatagta taatattatt gttattctgt ttataaaata
    20281 aaatttgata aataaaagcg atacttaggg aggattaaaa tgtcattaaa tatagtatta
    20341 gtagaaccag aaatacctca aaatacagga aatataataa gaacctgtgc agcaacaggt
    20401 tcaactttac atttagtaaa accactgggt ttttcattgg atgataaaca cctaaaaaga
    20461 tgtgggcttg actattggga tatagctgat atacaatatt acgatagctt tgaagaatta
    20521 caagaaaagt atccaaatag taaatacttt ttttctacaa caaaggctaa acagtcacat
    20581 tcagatgtga aatatgaaga aaattgtttt atagtatttg ggaaagaaac aaagggatta
    20641 ccagaacctt tgttgaaagc aaatatggat acttgtatta gagtgcctat gttggatgtt
    20701 gaaaaagcta ggtcattaaa tttatcaaat tcagttgcta ttgtagtgta cgaggcttta
    20761 agacaaatag gatacccaaa tatgaggtag tttcaacaag cctaaatgag aaaggagcat
    20821 acttaatgaa aaatctaaag ataatctgtg acagtttatc ggacgtgcca aaagatttgt
    20881 tagataaata tgatatagat atagtttcat taacagttat actggaggaa agagaataca
    20941 aggatagaat tgatatatct ggagatgaat tttataaaaa gcttagagaa gaaaatgtat
    21001 atccaaaaac ttctcaagct acttatgccc aatttaagga aatttttgag aaatatacaa
    21061 atgaggatag agagatactt tatatagcag ggtctgccac agctacaggt acgtatcaaa
    21121 gtgctgtaat ggcaaagaat gatgtagatg gtaaaatata tacatatgac tctaacttgc
    21181 tttgtatggg aactggttta cttgtaataa aagcaggaga acttgcaagt gaaggaaagt
    21241 ctgttgctga aatattgcct gttttggatg aattaaaaga gaaggctttt gttatatttt
    21301 cagtagatac attggaatat ttacaaaagg gaggtagaat atcatctact aaggcagcaa
    21361 taggaagtat attaaatata aaaccgattc ttgaaataag agatggatta gtttcgcaaa
    21421 tatctcaagt aagaggaaag aagaatgtaa tttctaagat gatggaattg ataaaatcaa
    21481 attgtggtga tgatttatca gaccaaattg tatatatagg atatagtgat gattttaagg
    21541 aaaaagaaca attatctaag gttatgcaag atgaattaag acctaaagaa attagatttt
    21601 tccaaattgg tgtgtgtata ggtgcacatg ctggtcctgg tgttactggt atcatatgct
    21661 taaaaaataa ataatatatt tttggatgac attatttatt tttttattaa attaggatag
    21721 aatattaatt attgagatgt taaattggat ttattttgga tgaaattaag tgttgatata
    21781 ttagatttag tttgtaggga agtgtgtaaa tagataagaa tctattgcac acttttttat
    21841 agtaagattt attaaaaaaa tttcaaaatg tacttgtaaa ttgtatataa aaaaattata
    21901 aataggatgt aaaagtaaat aggatgtaaa agtaaatagg atataaaaat atcgtaaatg
    21961 tatgaggagt gaaatttaat gcgtttcaaa agaatattga tattgatgtt aactgtagca
    22021 atgatagctg gaatgttggt tgggtgtgct aataacccaa gcaataatcc tagctctagc
    22081 aaagataata aaagtgatat aagcgataaa agtaattcaa aaaaatctgt atcaatgata
    22141 ctagatattg aaggaacaaa taatgaagct atgaataatt cagctttatt agcattaaac
    22201 aatgctcaaa agaaacttaa tatagataca aacaaagtgg aatcagatga ttcctcaact
    22261 ttttctaata gtatagatat tttatgtaat gacaattacg atttaattat agctgtagga
    22321 gctagatttg caaaaccatt ggagatggta gccaagaaat atccaaagca acaatttgca
    22381 attatagact atgagtatga caagcaacct tctaatataa cttcaatttc ttatgaagat
    22441 aataaatctg gatatctagc tggattaatt gctggaaaaa tgactgaaag cgataaggtt
    22501 ggttttatag gaggaacaaa gagttctagt agagataaat ttgaatcagg ttttagagag
    22561 ggtgttaaat tttctaatag tagtataaag gatatttcag ttgagtatgc agatgttttt
    22621 aaagatagca aatcagtaga atctatagct aaaaaaatga tggataatgg agtggatatt
    22681 attttttcta ctactgaaga tgattcaaaa gctgtaatag atgcagtaag ggcaaaaaat
    22741 aaaaaagtta tagcaacgaa taaagaccaa catgagttag ctccagaaaa tgttataagt
    22801 tctattgtaa aagattttga gaatcctact tataatctga tacaaagttt tgtaaaagga
    22861 aattataaag gcggaaaagt tatagaaaac acagttaagt ctggaagtac tgggcttgcc
    22921 caaaatagtt ctcaaaatat tcctccagat gtgttagaat atgttaataa gaacaataaa
    22981 taatctacta taatatccaa gaatacttat gactttagtt atgaggtgaa ttggatgtta
    23041 taatagacat atatagaaag acatatgcag atgtaggtca gacctatcta aatcagggag
    23101 tgaattttgt gaaaataaaa aggatattga tggcttgctt tgtagtagtt tttacaattt
    23161 ttatattagc tatatgtaat tttgattttg ggtttgataa agtaaataag aataattcta
    23221 atatagatag tgctaaatcg aaacagagtg agtctaaaag tttgccaact tcagcagaaa
    23281 aggataaatc aaatagtaaa agtaagaata aagaagatga cacagaagat gattcatatg
    23341 taagaaaaac tattggagta ttgttagata ccgaaaactt aaataacgaa tcatataata
    23401 acaatgtact atcagcctta aaaagggctg acgaagattt tgatgttagg ataaaacttg
    23461 aaaaaccaaa gacttctaaa agttttaaag agagtataga aaaactgcat aagtatgaac
    23521 cagatttgat aatagcagtt ggagccagat ttgcagagcc aattaaagaa gctgctactg
    23581 aatatcccaa acagcaattt gcaatagttg actatactta taaagttcag ccagctaatg
    23641 taatttctgt atcatttcaa gataatgtta ctggatattt agcaggactt attgcaggta
    23701 aaatgactga tactgggaag gttggtttta taggaggagt tgagggttct agtagagata
    23761 agtatgaatc tggatttaga aaggggctta aaacttcaaa taaagatgct gagttactag
    23821 ttaagtattc aaatatattt gaaaaccctg aatcagctag aactattgca aaggaaatga
    23881 gagattctga tagagatgtt gtattttcag ctacagagga tgatagtaaa agagctattc
    23941 aggtagcaag agaaaatgga ggtaaagtta tagctattaa taaagaccaa tacgatatgg
    24001 ctccagataa tgtaataagt tcaattataa aagatgtaga acaacctgta tatgaactga
    24061 taaagaacgt tgcaaaagat ggttttaagg gaagtaaagt aatggaattt aaaataaaag
    24121 atggagaact tggcttgact tcaaagagca agcagtagaa atataccacc agatgttttg
    24181 gaatacataa aaaaagagta taacaaagtg aaagatacat attaattatt gatgtagaag
    24241 attataaaaa tatactgctt aagtttagat tggtgataaa aggatatatt tattaaattt
    24301 gtgctaactg tacaaaatat attgattaag tttagattag tgataaaaag atatatttat
    24361 taaatttgtg ttaacagtat ggaatatact gcttaagttt agattggtga taaaaggata
    24421 tatttattaa atttagtcta actgtacaaa atatattgat tgagtttaga ttagcaataa
    24481 aaagatatat ttattaaatt tgtgctaact gtacggaata tactgcttaa gtttagatta
    24541 gtgataaaaa gatatattta ttaaatttga accaactgta caaagctata ctaattaagc
    24601 ttagattaac tatataaaat tataacaata agtgtgtggg cagaaaaatt ctgctacaca
    24661 ctttatttat attcacataa aaaatttatc aaaaaaaaga aaaaggagta aaaaatggaa
    24721 tttacaagag aaccatttat actagatgac gatttgtgtc tagctttggt ggacaaaaga
    24781 attagttatc taatggaaaa tgaattaaaa ttaagaaata tagagataat aaaaacaata
    24841 gaatgtaaag aattatatga aagcataaaa tatcacccgg atatatgtat ttgtaattta
    24901 ggaaaaggag atataattgt tgcgccgaat gtatataatc aatacaaaga gttattaagt
    24961 agatataaat ttaatataat taagggagaa gctaatttat taaatagata cccttatgat
    25021 ataccttaca atattgcgat agttggtgac tatgctatac acaattttaa gtatactgac
    25081 aaagcaatat tagattatat tgaaaataat aaacttaaaa agataaatat agagcagggc
    25141 tattctaaat gctcaatctg tatagtagat agaacgtcta taataacatc agataaaggt
    25201 atttggaaaa gtatgaagaa tacagaaata gagtgtttat tgatagaaaa aggtcacata
    25261 aatttatttg agatgaatta tggatttata ggtggatgta cagggttgat ttctaaggat
    25321 aaactagctt tttgtggaga tgttaaaaaa catcctgact atgaacgtat aaaaagtttt
    25381 gtagaaagta aaaacaagga gatagttaca cttagctgtg aaaatttact agatataggt
    25441 tctatagttc ctctcatgac aagaaaggag cgataacttt ggttgagaag gataatattt
    25501 cttccatgaa atttttgagg aataataatg taaacgatag attaatatat acgaatgaca
    25561 ttgtagaagt agtgatagat tctgcaaagc aagaagtgtt aaataaaaaa atacttaatg
    25621 gtactaaagc tgaacaaaga gctttaaata ttgatgagat atcaacggaa ataactagta
    25681 tgataataag tataatgaaa gagaaacttt tgaaagaata cattctaaaa tgctatgggc
    25741 gtacatatcc tgaagataaa aataatatat acatatattc actgaacata tttgctaaaa
    25801 aagagatttt tatgagagaa acagtattta ctagggtgag aaattatata gaagaaaatg
    25861 attttataaa tgtagaaggt tttattagat ttagaatgag agaatttatg aaatatattt
    25921 ctgctatagg tgatattgca gttgaggagt atttaataaa gaaagaccaa gatgaattta
    25981 taagagtttt aaaatacttt atagatactc aagaagaaaa gattgatttg cttaaagttc
    26041 atataatgga agataataca tttgttttat acgataaaaa tggaaataag atagacagta
    26101 tagatgatga agaaattata aatatggtaa taagagaaaa tttaaattat gaggactttt
    26161 taataagtac cttattaact ctatgtccca aaaaaataga aatattggat ttgttaaata
    26221 ataattgctc aaaggaaatt atagatactg tagaagcaat ttttgaaaat aaagttagta
    26281 taattatgga aaactagaat ctaagcatat aaaagctatc catataaaat taaaaaaatt
    26341 ctttttaaat cgacacaaaa gacttataat tgtatataaa gctatagtat aactagtgtt
    26401 attaagtata aagaatatct acttgattaa tacaatatag ctggcaaata tacaattgat
    26461 aaggagtaat tttataatgg ataatttaat taggatactg atattagctg ttattggtgg
    26521 atttataggt tatgttacga atgtagttgc tataagactg atatttagac caatagagcc
    26581 tattaaaata cctattttaa atatagaaat agtaggtctt ataccaaaga gaagagcaga
    26641 aatagcagca aatgtaggag aaataattca agaagaattt ctttctatgg acgaaatact
    26701 tgcaaatatc attactgacg aagataagga agaagttgta agatacataa aagcaagggt
    26761 aaaaattata atacatgaga aagtttcatt tatcccgagt ggtataaaaa acatgataca
    26821 agattatcta ggagagataa ttgagtctga agttaagcaa agtatagatg aattgagtaa
    26881 aaatataata aataaagcaa atgaacgtat agatatacaa aaaatggtcg aagataagat
    26941 taatgaacta gatttatatg aattggaaga gataataata agaatagcta agaaagaatt
    27001 aaaacatata gagtttttgg ggcttgtctt agggttttta ataggtatag ctcaagggtt
    27061 aattactatg tttatttaaa tatatgagaa gtagcattta tttaagtact aattatattt
    27121 acctatttga gtgaattact tattaatagc atttagatat taattgtatt tatttatttg
    27181 attgtgttac ttattaatag tatttaagta tttactgtac tacttattaa ctatattaag
    27241 tattgattgt atttaagtat acaaaaccta aaaagagtac aaaaattaga agttagtttt
    27301 aataaaaatt agttttagta tttatctagt catatctatc atgcatgtgg atacgtttta
    27361 aaaattttaa atttatgaaa atattataat atcactataa atcaagagat taatttaata
    27421 taaaaatata agaatattgg tttacagctg attgagatga aaaataatgg gtaattaata
    27481 tattacatta atgttgattg atgtataact atctgttata atgaaaatat attaaaaatc
    27541 atagtgttga agaggaagag tagagtagaa accattctca gagaatttgg gtctatggct
    27601 gagagcccat gtaataggtc tatttgaaaa ctaccttgga gctgagctta ttagcagtat
    27661 ttgttttgta ctatacgtga aagtgctcgg gtgacgacct tatagtcatc aagagggttg
    27721 tatatacttg tatacgccaa cttgggtgga accgcgaatt aatatcgtcc caaacatatt
    27781 tggggcgatt ttttattaca aaaaatctaa aaattatttc aataaggagg aaaaaacaat
    27841 gattaaagtt gctttaaaag atggctcaat aaaagaattt gaaaatgcta tttctgttat
    27901 ggatgtagct aaatccatta gtgaaggatt agctagaaat gtggtagcag cgtctgtgaa
    27961 cggagaggtt gtaggtctgg accacattat agatactgat tgtgacttga atttatttaa
    28021 gtttgaagat aaagaaggta aggaagtttt tagacataca tcagcacata tattagctca
    28081 agctataaaa agactttacc cagaagcaaa gcttgctata ggaccaagta tagaaaatgg
    28141 attctactat gatatagatt tagaccatag attagttcca gaggacctag agaaaataga
    28201 agctgaaatg aaaaaaatag ctaaagaaga tttaaaaata gaaagatttg aacttcctag
    28261 aaatgaagct ttagagctta tgaaagaaca aggagaagac tataaggtag aacttatatc
    28321 tgatttacct gaaagtgaaa taatatcttt ctacaagcaa ggtgatttta ctgatttatg
    28381 tagaggacca catttaccat ctactaaaaa ggtaaaagct gttaaattac aaagtgtagc
    28441 aggtgcatat tggcgtggtg acgaaaataa taagatgctt caaagaatat atggaacttc
    28501 ttttgagaaa aacaaagact tagaagagta cttacattta ctagaagaag ctaaaaagag
    28561 agaccacaga aaattaggta aagaattagg attatttatg atacctgaag aaggtccagg
    28621 attccctatg tttttaccaa aaggtatgga acttaaaaat gaattattaa aattctggag
    28681 agaaatacac agaaaagctg gatatattga gatagaatct ccaatcatat taaatagaaa
    28741 attatgggag acttcaggac actggtatca ttacaaagaa aatatgtata cagttaaaat
    28801 agatgatgag gattatgcta taaaacctat gaactgtcct ggtggattaa tttattataa
    28861 ctctcaacta cattcatata gagatttccc aatgagagtt gcagagcttg gtagagttca
    28921 tagacatgag ttatctggtg cattacaagg attaatgaga gttagagcat ttactcaaga
    28981 cgattcacat atattcatgt taccagagca aattaaggac gaaataaaag gagtagctaa
    29041 cttaatagat ggtatataca aaacttttgg atttgaatat aatttagaat tatctacaag
    29101 accagagaac tctatgggaa gcgacgaaga gtgggaagca gcagaaaatg gattaagaga
    29161 agctttagaa gagttaggtc taccatatac tattaatgag ggtgatggag cattctatgg
    29221 tcctaagata gacttccatc taaaagattg tttaggaaga acttggcaat gtggtactat
    29281 ccaattagat atgcagcttc caagacaatt tgataatact tatattggtc aagatggtga
    29341 aaagcataga ccagttatga ttcatagggt tgcttttggt agtatagaaa gatttatagg
    29401 tatattaata gagcattatg ctggtaaatt cccagtttgg ttatctccaa ctcaagttaa
    29461 gatacttcct atatctgata aatttatgga ttatgcaaat gaagttaaga aagagttatt
    29521 tgataagggt ataagagttg aattagatga tagagctgaa aaaattggat ttaagataag
    29581 agaagctcag cttgaaaaag tgccatacat gctaatagta ggtgaaaaag aagtagctga
    29641 taataatgtt tctgttagat caagagataa aggtgaaata ggtagtatta aattagatga
    29701 atttatagct agtatttcaa aagaaattga atcaagagaa agtattattc aagactaata
    29761 tatattatat tagtagctaa gatattgtta tataagataa gtttaatcaa tatgttgata
    29821 tgttgatgca tagtgattaa atttatgaaa agatgagtat aattagaggt acagttaatc
    29881 atatttttat gattaatgta cctctagttt tttgtatggt agatttatta ataaatatat
    29941 catagaagtg ttttgaacat ggaaatattt atgtgcattc tttaaataaa cgtttatttt
    30001 ttgagtgata attaagtgct tgttggtaag gttatggctg taaaaatcat aactttattt
    30061 tcatatttgt tccattttca taaaaattat tatcccttta agaaaatttc ttaatcctct
    30121 tgaaatattt cctttttagt ctaaagttaa atttctttct tcaaaatgaa tatttaaatg
    30181 aactaaaatt taagtacatt ttctaatctt atgagtctat ttttcataga taattgccct
    30241 ataatagaag aatctatgtt ggtgctatgt ggtgacaata acagcattgt cagtgattaa
    30301 ttaatgcttt ataaatgatt aattagtgtt ttatgttatt tttggactta ccctctatac
    30361 atagattaat ttatttaact ttagatagag agagaatgtc aagtacatac acagacatta
    30421 gagtttaaga aagtgtttat ggagcaagaa cttttaaaag aggtagtgac ttagtttaat
    30481 ttcaagaaat gataaataca aaagaggaaa aagagaggtg ttgaaatgat agaaaaagta
    30541 gaagaaaaat ctaagattat taaaacatca aaagaatata agcgaactat aattgaagaa
    30601 aaagataaag aatggaccaa aaaattaaat gatatactta gtagaagtag tttttctaat
    30661 gatgaattag ataaaatcaa ggcgttaatt ccaaaagaga ttttgactaa tgaaaatgtt
    30721 ggagaagttg tgactgaaga tggatatgct aagcccgagt atgatgaggt atttaaatca
    30781 ttgttcactt taaataatga atatgattta cttgtaaatt ttataaatga tattctaaaa
    30841 gatgctccat atgctaacag gaatatcaaa ccgttcactc aaataaagag aataattaga
    30901 gttgaaactg atccaacaat taattacatt ggagagaaaa gacctaaact tgatattttg
    30961 gctgaagatg aagaaaataa tcatataaat attgagatgc aaagagcttt tgagaacgat
    31021 tatttggaaa gagctgaata ttatcttagt cgtgtacatg gtagaaagtt agaagaaggt
    31081 aaagagtata aagaaattgg aaaaactatt ggaatacata tactaaatca tgtaaaatat
    31141 aatcacatag aagattatgt aaattgcctg agattaacaa tggacgggca tccagatatt
    31201 tattctagta aaactgcatt atattttata gagcttccaa aaattcataa atctgactat
    31261 atagtaaata gaattatgct atggggaaaa cttattagta atccttctaa cttagatgta
    31321 agagttatag ctaagaatga ttctattatt aaaaaagcac ttgatagatt aaaagaatta
    31381 ggtagcaatg aagaatattt attaaatctg aaaagaggtg cttatatcat gaataaaagt
    31441 agaaatttta aagaagagat tgaaagagag acagcaatta gagttgcaaa agaaaaagat
    31501 tataaaataa ttataatgtt gaaaaaaaat ggttttaaat tagatgacat attaaataaa
    31561 tttaatgatt ttgacttaag tgaagatgag ataaaagaag tatatgaaaa taattgatga
    31621 taaataaaaa gtgaacttat ataagtttgc tgaatcggag tctggaaata tttatgtgta
    31681 ttttttaatg aatgaaaatt tattttaaaa acaataattt agaagtacag ttaattatat
//
LOCUS       DACULQ010000022        31463 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.022, whole genome shotgun sequence.
ACCESSION   DACULQ010000022 DACULQ010000000
VERSION     DACULQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.022"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(174..743)
                     /locus_tag="JA154_04400"
     CDS             complement(174..743)
                     /locus_tag="JA154_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAT7995099.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            946..1173
                     /locus_tag="JA154_04405"
     CDS             946..1173
                     /locus_tag="JA154_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995100.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(1392..2135)
                     /locus_tag="JA154_04410"
     CDS             complement(1392..2135)
                     /locus_tag="JA154_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HAT7995101.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNATSQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            2303..3175
                     /locus_tag="JA154_04415"
     CDS             2303..3175
                     /locus_tag="JA154_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7995102.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            3175..3840
                     /locus_tag="JA154_04420"
     CDS             3175..3840
                     /locus_tag="JA154_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HAT7995103.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            3861..4718
                     /locus_tag="JA154_04425"
     CDS             3861..4718
                     /locus_tag="JA154_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7995104.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(4874..7420)
                     /locus_tag="JA154_04430"
     CDS             complement(4874..7420)
                     /locus_tag="JA154_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7995105.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(7420..8106)
                     /locus_tag="JA154_04435"
     CDS             complement(7420..8106)
                     /locus_tag="JA154_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7995106.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(8189..9178)
                     /locus_tag="JA154_04440"
     CDS             complement(8189..9178)
                     /locus_tag="JA154_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7995107.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(9165..9851)
                     /locus_tag="JA154_04445"
     CDS             complement(9165..9851)
                     /locus_tag="JA154_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7995108.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(10351..13557)
                     /locus_tag="JA154_04450"
     CDS             complement(10351..13557)
                     /locus_tag="JA154_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAT7995109.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(13750..14799)
                     /locus_tag="JA154_04455"
     CDS             complement(13750..14799)
                     /locus_tag="JA154_04455"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090113.1"
                     /note="catalyzes production of carbamoyl phosphate from
                     bicarbonate and glutamine in pyrimidine and arginine
                     biosynthesis pathways; forms an octamer composed of four
                     CarAB dimers; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HAT7995110.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(15056..18262)
                     /locus_tag="JA154_04460"
     CDS             complement(15056..18262)
                     /locus_tag="JA154_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAT7995111.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNHRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(18315..19358)
                     /locus_tag="JA154_04465"
     CDS             complement(18315..19358)
                     /locus_tag="JA154_04465"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090115.1"
                     /note="catalyzes production of carbamoyl phosphate from
                     bicarbonate and glutamine in pyrimidine and arginine
                     biosynthesis pathways; forms an octamer composed of four
                     CarAB dimers; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HAT7995112.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(19384..20100)
                     /locus_tag="JA154_04470"
     CDS             complement(19384..20100)
                     /locus_tag="JA154_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAT7995113.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(20476..21063)
                     /locus_tag="JA154_04475"
     CDS             complement(20476..21063)
                     /locus_tag="JA154_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HAT7995114.1"
                     /translation="MDKLMKICPTREIELQQKAKQLYEEEENRKIAYNAGLVEAEGYC
                     QYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHHGFEVFSVICKNGAMPK
                     STIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGLCVGHDTLAMKYMEAPI
                     TTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(21135..22049)
                     /locus_tag="JA154_04480"
     CDS             complement(21135..22049)
                     /locus_tag="JA154_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995115.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            22650..23879
                     /locus_tag="JA154_04485"
     CDS             22650..23879
                     /locus_tag="JA154_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAT7995116.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWNIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(24084..26465)
                     /locus_tag="JA154_04490"
     CDS             complement(24084..26465)
                     /locus_tag="JA154_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HAT7995117.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(26437..27162)
                     /locus_tag="JA154_04495"
     CDS             complement(26437..27162)
                     /locus_tag="JA154_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HAT7995118.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKYVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(27152..27607)
                     /locus_tag="JA154_04500"
     CDS             complement(27152..27607)
                     /locus_tag="JA154_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAT7995119.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(27889..29043)
                     /locus_tag="JA154_04505"
     CDS             complement(27889..29043)
                     /locus_tag="JA154_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAT7995120.1"
                     /translation="MFRPILDLIISCALTSMLILTSMYIIKSLYVNSDMSVVNQLVYQ
                     ARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQKLEIIKSAV
                     NLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIEERQAQQTKT
                     DLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNLNVLINDLFE
                     LTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKLIVKADPNKL
                     VRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLPNIFDRFYRV
                     EKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQSA"
     gene            complement(29060..29752)
                     /locus_tag="JA154_04510"
     CDS             complement(29060..29752)
                     /locus_tag="JA154_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7995121.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(29886..30602)
                     /locus_tag="JA154_04515"
     CDS             complement(29886..30602)
                     /locus_tag="JA154_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HAT7995122.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            30727..31461
                     /locus_tag="JA154_04520"
     CDS             30727..31461
                     /locus_tag="JA154_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAT7995123.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
BASE COUNT        10026 a         5628 c         3813 g        11996 t
ORIGIN      
        1 aatattacct aacatctaca ataaaatata ctcttaaaat aaaattatat aattaaaaat
       61 actctataaa agctaaaaag tttttctatt cgctacatct aaaaataatt agatagaggt
      121 ataaatgtat ttagtttatt tatacctcta gtcttttata aattatatct ctactaaatt
      181 gattttttag cacgaataag tgtgctcttg gagataccag tcatcttcac aacctctcta
      241 tatggcttag aatttaataa ccccaatgca tattgtatct gttgattgct gtatttcttt
      301 ggcctaccat ctttaaagtc ctttttcttt ttagcaaatt cttttcctaa ttgagtacgt
      361 tctattatca tatttctctc aagttctgca actgcaagta atgtagtcaa aaagaattta
      421 cccattgaag tattttctaa aaggcctata tttaaaatat gaacacttac ttctttagca
      481 aataaacctt ctattagttg tattccttct gatgtacttc tagctagtct atccagctta
      541 gttgcaacaa gtaagtctcc cttttttaga gatgatataa ttttttcaaa tttaggcctg
      601 tttgtagtag tgcctgtata agtttccttt attattttag catcattata tttattcaaa
      661 atttcttgct gttgctgata caaactgttg ttgtctgttt gattttttgt acttacacgg
      721 caatatccat atataataat catcacttcc taacataaag attttgtaca gaagttaccc
      781 agaagcttgt ttttacttat ctttatccat tttactaaaa cgatttttgg gtgtcaatac
      841 tgttaacttt tgacaccata ataattcctt tataatttgt ttgactatgt caaaaattac
      901 caatataatg aaattaatga gtttatacaa attggaggaa ataatatgaa tagtctgtta
      961 aaagaagcaa tagatgaatt aaaaaacatc cattgcgggg aagttttttt attaaaagaa
     1021 ttgttcaaag gctatgaatg gaacaggttg gagatgaata tcagattgaa attaggaaaa
     1081 ctttttttgt atgaagtggt aactaatccc aatttaaatg tagaggtcat caataaaaat
     1141 tctgcttatc agcaaatgta taaaaaaaaa tagtgactta aatgtatata atagtataat
     1201 cttcttattg attcaaaagt taaaactttt aatacaaaac aatcattcta ttttaacatt
     1261 tatcaatatg tctaaattta tataattaac acatacgaat agtattgttt aaatcacatt
     1321 atttaagata gataaatctt aaccctactc cagcataaat actaaaatag ggcttagaat
     1381 tatctatatt tttatcccat aaaatgctta gctcttcctc ttgattgaac atatatatct
     1441 ggggaaatac aatagaagcg taaatttata aaatttccaa agtcagaaaa cttcagttca
     1501 aacttaggta taccaaaact ttcaggtgct atttcgtcaa cgccaaaata gatgataatt
     1561 ccattatctg taagataaaa tgcttggtct tcaggtataa ctaactcaac attagaataa
     1621 tacatttctc tattttcatt tatttgagat ttaatgtaat cagtaattat attaatataa
     1681 tcaactccat ctctaaaaat gtcattaagt aaaagttctt ttccagttgt caaatctata
     1741 ttgtaattat tcaatgtatt gtatttaggc ccatagttat tagcaaaccc cataaggttt
     1801 aatatcatac tcagtacata atttttatta aaagtaactg cgtaactggt agatacccaa
     1861 tagctttgtt ttggaagtga attttgagaa gttgcgttat tatattctcg ttcttcttcc
     1921 tgtaagccat ttttaaaatt atttacagtc attcttacag cattgtttat gttatttaaa
     1981 ataactgggt ttacatacgt ggattgattt ctaatattgt atataacagg atactttaca
     2041 tcatatgaaa aatacttact tctttcaaaa tcttcattaa attgtacata taatatatta
     2101 tatgtacagg tatttgtaga ttgataattt tccattaaaa tcctccccct tttataaaaa
     2161 aatcagatac aatataatat gcttaaaaca gtaataatgt taatggtaaa tgtaaagaat
     2221 agaaagtatc taaaacagca aaaaatgact aattttgtgg tataatacat aggattgaga
     2281 gttattttat aggaggaaaa atatgttatt attagcagat aaatggaaag attatgaact
     2341 tatagatatg ggaaatggag aaaaattaga acgttgggga agttatgtac ttagaagacc
     2401 agacccacag gttgtatggc caatggaatc tgaatgggct ttatggaaaa accctcatgg
     2461 acattatcac agaagcaata aaggtggagg tcaatgggaa cacaaaaaaa gatatccaga
     2521 acaatggact ataaattata aaaacttaaa gtttcacatt aaaccaactg gctttaaaca
     2581 tactggatta ttcccagagc aagcagctaa ctgggattgg atgatagaga aaataaagaa
     2641 agctaacaga cctataaaag tgttgaatct ttttgcatat acaggtggag ctactgtagc
     2701 atgtgcatct gctggtgcag aagtatgtca tgttgatgct gcaaaaggta tggttaattg
     2761 ggcaaaagaa aatatagaat tatcaggtct taaaaatcaa acagtaagat ttatagtgga
     2821 tgatgttgta aagtttgttg aaagagaaat aagaagaggt aaaaaatatg atgcaataat
     2881 aatggaccct ccttcttatg gaagaggtcc taaaggagaa gtatggcaaa tagaagaaaa
     2941 gttatacagt tttgtagatt tatgtatggg agttttatca gataaccctt tatttttctt
     3001 aattaattct tatacaacag gattttctcc aatagttctt gaaaatgtat tgtctacaac
     3061 aataggtaaa aagacaaata caggagatat atacggtggg gaattaggaa taccagcttc
     3121 cagagatggt aagatacttc cttgtggtat atttggaaga tgggagaaaa aataatgata
     3181 aaagtcatat atgaagataa ccatttatta gttgtagaaa aaccagttaa catattgtct
     3241 caaggagata atactaatga taaagatatg gtaaatcttt taaaagacta tattaaaaaa
     3301 aagtataaca aacctggaaa tgtatatatt ggtttaatac atagattgga tagaccagta
     3361 ggtggagtta tggtgtttgc aaaaacatca aaagcagcat ctagattgtc tgagcaggta
     3421 agaagtaaga ctttcaaaaa aacttatcta gctgttgttc atggcaagat aaaatctaac
     3481 tcagatatac taaaagattt tttatataaa aataaaaaaa ctaatatggt aactgtagtg
     3541 aaaaaaagcc ataaagatgc caaagaagct gaacttaact ataatgtatt agattttaaa
     3601 gaaaatctaa gtttagttca agttaattta aagactggtc gttctcatca aataagagtt
     3661 caatttgcaa gcagaaaaca tccattatac ggagaccaaa gatatggcaa agaaataaat
     3721 aagattggac aacaaatatc tctttggtcc aataagatag aaatttgcca tcctactaca
     3781 aaagaaaaaa tggaatttac atgtcatcca cctgacaaat atccatggaa tatattttaa
     3841 atctcaaatg aggtgattat atgagtaaaa aattttcaat aggagaaatg tctaaacttc
     3901 ataaaataag catacagaca ctaaggtact atgaccaaat agatttattt aaacctgttc
     3961 atgttgacca agaaagcaat tacagatatt actcaattga ccaattttca caattagata
     4021 ttataaaata tttaaaatat ttaggtatgc ctttaaaaga aattaagtca aagcttgatg
     4081 aaaataatga agaaattcta aaattactaa caaataaaat taatatagtg gatgaaaaaa
     4141 ttaaagaatt agagcttata aagaaggtac taagcacaaa gaaagaaact atggaaaaag
     4201 gtatgaaagt agatgaagtt ggtaaaatag tgagaaaaca tattccacga agaagtatct
     4261 tatctataga gtatgaagaa ggattaagta gtgaagaaac tatggaactt tccagaagac
     4321 aaatagcaaa tatattagaa gataatatat ctgtgttcta tggtggagta agtggacttg
     4381 tatctataga taaattttta aaagaagata aagttattta tgacaatagt tttgttactg
     4441 ttgaaagaga cttatttgat ataaaatcac aaaaacatat aacagaaatt tcatctggtg
     4501 attttatttg tatgacatat aaagggcctt acaaagaaaa ttataccgct tttaagaaac
     4561 tggttgatta tgttgaatat aaaagaatac ctattgaaaa ttatatatat gaaataccta
     4621 taatagaccc tctgtctaca aacaacgaag aggaacttct aacagaaatt caaatacaag
     4681 ttaaaaagag agaaattgac ttaagatgtc tagtgtagta atctagttta cttacacatc
     4741 ttaatatagt tgtctattat caacatatga tttttctaga tagtgattcc taacagtgag
     4801 attttcaaaa aataggagga aatatggcta atgctaattc cctcctattt ttaattaagc
     4861 catatcattt taattaagcc atgtcatcac gaagtgcatc aataatattt tcttttttta
     4921 tcttattaat agtatacaac attgtaataa atactataaa taaaacacta aatatactaa
     4981 ttccaatact aaaccatgga atcacaaaat caatatcatc tgtaaccatt actttatgaa
     5041 ttagccatga agaaattact gctataggaa gtccaaagaa caatgctctc atgccataaa
     5101 aggcacactc aaaattcatc atcttttgga aatcatgctc agacattcct acagagcgaa
     5161 gcatggcaag ttctctacga cgtaacttaa tattagttga aattgtatta aacacattgg
     5221 caatcgaaat cagagaaatc ataattatga aagtataagc aaaaacattg gcaataaaaa
     5281 tgtaattacg gttctcatcc atcattccat acatattgta cagattgtac tcattcgtaa
     5341 ctccctcact ctcaataatt tgctccattt ctgcaactga ctgagtagga tttttcgatt
     5401 gaaagcttaa actttcaatt cctgtagaaa caccaggaat ctcaaactta tctttggctg
     5461 aataaggagc caatactctg aagcttgctt tcttcacatc tgaacttctg attcctggag
     5521 gagaatcagg tggcactact ttgacaaatg aaatattcac attttgtctc tcaggtatct
     5581 ccaacttgcc attagtttca ggagtgaggc taacattcat agaagaactg ttgaaaatat
     5641 caataatatt attatccttt gttaccatag aaacagcaat cagttttgca ttttgtccag
     5701 tatattcttc tgctggtaat ccaagacttt taataatatt cagataagtt ctatcatcaa
     5761 gaaattgcat atctactggt aattcaagca tttcactcat agaatgtgaa ccctcatagt
     5821 tcaaataatc gtctgtaagc tcacttactt tggcaacaca tgaataggtc ataagtttct
     5881 gatacaaacc ttcgtaaaca ccatttgcag tttttagctt atcgtaaagc ttcaacattt
     5941 cgttgtcttt cataccttgt gtagaaaaaa taatatcctg gtcagtaacc tctgacctct
     6001 gatttgacat ctgttttagg tctgctacta aagcacttgt agatataaat agaactatgc
     6061 ttaaaacaag cgacaataca atacttcgat aacgtttctt gtttctttta aagtttttca
     6121 tagcaagaac tccctcaaga ccataaactc gctgtgttag ttttgatgtc ttcacagatt
     6181 tagattcaat ttttacctca tttgtctgac gaatacactc catcactggt gtgttggcag
     6241 cctttcttgc tggtatgtaa gcagaaatta agattgtaac catacttacc actgctgcaa
     6301 cgatgattgc agggatagac aatgttaatg tcaacggaac gtttacatag agaatatttg
     6361 caaagttttt agctatgaca gaaatcacaa gtccaatact gactataccg attgcaacac
     6421 caattggtat accaatcgca ccaatgcaaa ttccttcaaa tagcaccgaa tttcgtaact
     6481 gttttgctgt agctccaaca gatgaaagta ttccaaattg atgtgttcgc tcgttcaatg
     6541 acatgttaaa tgaattatat attagaaaaa ttgagcctag catgattatg gcaagcacaa
     6601 tgccaccaac cgagtacaaa aacatattga acaaattgtt atcagaaaca cctatgaagc
     6661 gcaacacatt gtcattgaaa atggaagcat ggtctttagt gctacttatg taagagcgaa
     6721 tctgatgtgg atttttcaat gtgacaaaaa ggctaagact gtcagtcttg acttgtttat
     6781 cttctttggt tatcaaggta tatcctggtg cagaagattc ttcaaatacg ggctttctac
     6841 agatacccac aactgtataa gttttcttat cctgtggcac aaaaacttca ctcccagcag
     6901 tgtatgggtc actctgacca agcttttcat tttcactcat tcgtttccct acatccaaag
     6961 aaatagtatc acctactgca aatttgacac caccatctgt cgcaacttta cttgaaacaa
     7021 gaatctctct actattttca ggtaatcttc cagaaattaa agttataggc aaagtatcaa
     7081 aagcttcttt gctaaatcca gcaataaaaa gatatggttt atctggattt ctgttacctt
     7141 caagtgttgc atagccaata ttttcaaatg ttgcagtatt tgcaacttca tcattgactc
     7201 tttgttcctg tacaaaagaa gaatccacat ccaaaaactg gacatgccaa tcaccatatt
     7261 ttataatagc tccatttgtc atatagttca tcaaagaaac tccaaaggta gtaactgctg
     7321 taatcatagc agctgacaga acaactccta taattgttac aagagttcga gtgcgattct
     7381 ttttcaatcc ttgcagagca actttgttaa aaatgttcat taacgattca ccttcctctc
     7441 atcacgcact acttttccat ctgaaataca gataattcta tctgcctgta aagcaatgtt
     7501 ttcatcatgt gtgacaataa tgagagtttg gtgatattta ctatggctct ctttcagtaa
     7561 ctggataatc tcttgtccat ttttactgtc taagctacct gtaggctcat cagcaagcat
     7621 aactgctggt gcattcatca atgcacgacc aatggcaaca cgttgctgtt ggccaccaga
     7681 aagttgattt ggtaaatgtg tttttcgttc ttttaaacca agcaattcta acaagtcatt
     7741 taaccgttct tgattgacct tacgcttatc cataagtata ggcaatgtga tattttctac
     7801 tacatttaat gtgggtatca gattgtgaaa ctgataaatc aatccaactt gtcgcctacg
     7861 aaaaatagca agtttttcat tgctttggcc atatacatct tgcccctcca gatatatctt
     7921 tccacttgtt ggtacgtcta ctcctgcaat ggaatgtagt agtgtagatt taccagaacc
     7981 agaagaacca atgattgcag taaactctcc tttttcaata gtaagtgaga catggtcaag
     8041 tgcagtaacc ttattttcat tcttaccata aaccttacat aaatcttcaa tctttaaaaa
     8101 cgccattata taagtctcct ttcatatttt ataaatatat cataggactt tcaacttaca
     8161 tttaagtgac atttaaatga gagattcgtc actttggaaa acgaataaca aatattgcac
     8221 caccttgtgg gtgattttgt gcagtaattg ttcctccttg tctcataata atcgtcttac
     8281 aaagtgccaa tcctattcca taaccggctg tgcttgaatt tttcccacga taaaatctgt
     8341 taaatagaca tggtaaatct tcttttttaa agcctgctcc gttgtcatga atagtaagtt
     8401 gagtaaacaa aaagttgtcc tcacaaatga tatcaatttt tccattatca cccacactct
     8461 ccatgcagtt tttaaatata ttttgaattg cttctgaaag ccaatttaaa tcacccaaaa
     8521 tccttactcc ttttggtata tcaatatgta aagtgatatt gtgcagttcc atgggaatca
     8581 aaagttgatg aagtgcagaa ctaatcaaat tattcacatc aatttgttct ttttgaaaga
     8641 ctacaatacc tgcatctaaa cgtgataatt ttaaaaggga agtaatcagc cagtccattc
     8701 gtataagtaa ttcctctgtt tctcgtatca actcttttct ctcatcttca tcagaagtat
     8761 tctctaacag tgacaaaata agagttactg atgtaagagg agtgcgaagc tggtgggcta
     8821 tgtcagctag tgagtcagca aggtgttctt tttctttttt caatgcgtaa ttttgctctc
     8881 taatacgtaa cgtcatttta gttatttcac tttgcagaat agaaagttca ccctcatctg
     8941 attcagcaat atacagatgg ttagcattat gaagcacaag attaatttgt tctgaaatct
     9001 gtgcaatact tttgtatcga tttttagtaa acacaaaaaa tgctattcca aaggtagtgg
     9061 cagaaacaat agaaagtatt ccagtcataa tactgattgc aaatcccagg gctacagtga
     9121 gaatagtaat taataagaat aaaatagcaa attttcgaaa ctctctattt cgaaacataa
     9181 cttcccccca atctataccc tgtcccacga acagtcaaaa ttatttgtgg acttgaaggg
     9241 tcatcttcta tcttctctcg caagcgtttg atgtacacag tcaatgtgtt gtcattaaca
     9301 aactcacctg ccgcatccca caattcatca agtaatctta ccctcgtaat aatactttta
     9361 ggattgctaa taaacaccaa taacaaacgg tattctaaag ctgaaagaaa aacctcattg
     9421 ccattctttt tcacaacacc ggttgccgta tctacataaa gcccacaaat ttcaaaagtt
     9481 gacccaaaac gcccactttt tcgtagtgct gttctaattc gcgcaatcag ttcacgtgga
     9541 cgaaaaggtt tagtaatata atcatctgca cccatattca gtccagtaac aacacttgat
     9601 tcatcaccgg aagctgtcaa aaagataaca ggaatgtctt gcttttcttt gatttctgtg
     9661 caaatcgtaa acccatttcc atcaggtaaa gaaatatcaa taagtgccaa atcaaattta
     9721 ttcttagcaa tctctacaag tgcttcactt cttgtggata catgggttac tataaatcct
     9781 tctgaattaa gcaaaagaat aagatttttg gcaattgctt tatcatcctc aactaaaaat
     9841 atccgtttca tttactttca tcctccttta ttttattagt tttatatatt ccagcagagc
     9901 aatagtacac tactatttta cttatcatta cacaattttc agtagtttct tttgttctta
     9961 tataatccat gcataatacc tccattgatt tattatattt cttataacat aaatacacaa
    10021 gataagacaa aaaacataat gtaagtgcaa gttttttaac tcactaactt ataaacatac
    10081 actcctacta acactacttt cttgatgaca catcttacaa gcttatattc atacatcaat
    10141 gaaaaattaa atttatccat ttattaaaat aaaaggcaat agcttctata gctaatgccc
    10201 tttatcttaa aatgatttaa atactataca atttttataa atggctataa ataaactcgt
    10261 atagaatata aaatttatct acagtctttt gttatacaaa atttataacc tcttatagaa
    10321 caaatttacc actttgcaaa cttatcactt ttactcagct atattataaa cttttaattt
    10381 atctttgttt aaatgtttct tcttaacttc tactaaagct tttagcgtat ccaaagatgt
    10441 cattatttca gtagaaaatt caatggctgt tcttctcatc ttaaatccat ctcttgtaga
    10501 gtcatttcct ttagttggtg tatttattac tatatcaact tgtttatttc ttatagcatc
    10561 taatatgttt ggtctaggct cttcaattct atttactata tcagcctcta ttccattttc
    10621 tcttaaactc ttcgctgttc cttctgtagc aacaaaagta tatcctaatt ctttcatatc
    10681 ttttgctatc tctataaact catctttatc atgattattt acagtagcaa gtactactcc
    10741 tctttcatca gacatatgtc tacctgctgc caagaaccct ttatataagg cttcttctaa
    10801 attttctcct acacctagaa cttctcctgt agacttcatt tcaggtccta gacttacttc
    10861 aactttagat aatttagaca ttgagaatac tggtactttt actgatacta attttggctc
    10921 tttgtaaact cctgtactat atcctaaatc ctttaattta gctcctaaca tacacttagt
    10981 agccaagtca actattggta ctccacttac tttacttata tatggaactg ttctactagc
    11041 tctagggtta acctcaatta tatataattc attttgaaat tctataaatt gtatattaac
    11101 cataccaagc acattaaggt ctaaagctat ttttttagtg tattctacta ttttttcttt
    11161 tatttcatca gatatatttt gacttggata cattgttgta ctatctccag agtgaactcc
    11221 agctctttct aaatgctcca ttataccagg tatcaatata tcttctttat cacatatagc
    11281 atctacttct atttctctac cagttagata tttatctata agtactggat ttttatggtc
    11341 tctatcaaat gcatcatcca aatactgaat tagtttttct tcgttataag ttatttccat
    11401 accttgacca ccaagtacat aagatggtct aactaatact ggataaccta actccttagc
    11461 aatttcaaca ccttcattag ttgtccatac accttttcct tttggtctat ttatatctag
    11521 tctctctagt aaatcatcaa atttttctct atcttctgct gcatctatat ctctaaagtc
    11581 tgttcccagt ataggtatat ttttttcatg taaaaactta gccaatttta tagctgtttg
    11641 tccaccaaac tgtagtatta caccttctgg tttttctttt tctattatat ttaaaacttc
    11701 ttcttctgtt aatggttcaa aatacaactt atcagatgta tcaaaatccg tacttacagt
    11761 ttcagggtta ttgtttacaa ttatagtttc tattcccatt tttcttaaag attttacaca
    11821 gtgaactgaa caatagtcaa attctattcc ttgacctatt cttattggac cagaacccaa
    11881 tactacaact tttctcttat cactaactac tacctcatca tattgttcat aagttgaata
    11941 gtaataaggt gatagagctt caaattctcc tccacatgta tcaaccattt tataaactgg
    12001 ttgtatatta tataaacttc tcaattcata taatttttct gggcttatct tcattaagtc
    12061 agatatacct ttatcagaaa atcccttttt ctttaattca tgtaaatatt ctttatctaa
    12121 gtcttctatt tttagaactt ttaacttttc ttcttgttct actatccatt taaatttatt
    12181 tataaaccac ttatcaacac cagttatttc tgatatcatt tctactttat agcctcttct
    12241 tatcatttca gctaagtcaa atagtctctc atcatcaggt actactactc tagcctttaa
    12301 ttcttctaat gttctacctt ctgatggagc atgaaccaat gaatattttc ctgtttcaag
    12361 tgatcttata cctttaagaa tagcagcctc aaagttactt cctatactca tgatttcacc
    12421 agttgccatc atcttagttc ctaattttct attggcatgt ctaaatttat caaatggcca
    12481 ctttggtatt tttactacca cataatctaa tgttggctca aaacaagcat aagttttttt
    12541 agttactgca ttttctatct catccaaagt ataacctaaa gctatcttag ctgctacttt
    12601 tgctattggg tatcctgtag cttttgatgc tagggctgat gacctactaa ctcttggatt
    12661 tatttctatt atcgcatatt ctaaactatg tgggtttaaa gctatttgaa cgttacatcc
    12721 accttgaacc tctattgcat ttattatgtc tattgatgcc tttctaagta gttgatactc
    12781 ttcatcactt agagtttgac ttggagcaac aactatactg tctcccgtat gaactccaac
    12841 tgggtctaca ttttccatat tacatacagt tatacagttt ccatttccat ccctcattac
    12901 ttcgtattct atttctttcc aaccttttat actcttttca agaagaactt gtccaacagg
    12961 acttagctgt agtccatgtg aaagtatttc tcttaattct tcctcagtat cagctatacc
    13021 tccaccagtt ccacctagcg tatatgctgg tcttacaact actggatatc ctatcttatt
    13081 ggcaaactca agacctgctt ctaaatttgt aactatatca ctaactatta ctggttgatt
    13141 tatttctttc attacttctc taaataaatc tctatcttcg ccctttttta tagattctat
    13201 agatgttcct ataactttta cattatattt atctaatata ccagcatcat gtaattctac
    13261 agctaagttt aatcctgtct gaccacccat acctgcaagt aaactatctg gtttttcttt
    13321 ttctattatt ttttctataa attctattgt taatggttct atatatattt tatctgcaat
    13381 ttctttatca gtcattatag tagctgggtt actatttact aatacaactt caattccctc
    13441 ttcttttaat gcttggcagg cctgtgttcc tgaatagtca aactctgcag cttgacctat
    13501 tattattggc cctgacccta atactaaagt tttctttata ctatctaatt taggcataac
    13561 tctttctcct tatctaaaca attttcacag ttcttatctt catcatctat acaatcaact
    13621 ttaattactt tattttatct taaattcaaa tttactcatc aatacatcta tttaatacat
    13681 ctatgttaat taaaccaaac tttttgattt aagttattct tgttttttag ttagctattc
    13741 taatgttatt tattttacta attctaaaaa tttatcaaaa atatagtcat tatcttttgg
    13801 tcctgggcaa gcttcaggat ggtattgaac actgtataca tctagtttct catgtctcat
    13861 tccttcaaca gtattatcat ttaaatttat atgtgtcact ttcatttgtt caggaacttc
    13921 tgaaacataa taaccatgat tttgagaagt tataaatact tttccttctt ctaaatcttt
    13981 aactggatga tttccacctc tatgcccaaa tttaagttta gcagtttttc ctccaagagc
    14041 taaagctaaa agttgatgtc ctaagcatat tccaactata ggttttttac caataagagc
    14101 ttttatattt tctataacat cttctaggtc ttctgggtct ccaggtccat ttgacaagaa
    14161 tattaagtca gggtttatac tcaatacatc ttctggagat gttgtagctg gaaatattgt
    14221 tatatcacat ccacgagcta taaatgatct taatatattt ctttttactc caaaatccat
    14281 aacagccact tttggaccat ttccctttat atgctctact tcttttcttg taacagttct
    14341 aactgcttct gtatttgaaa acttatccaa tttatgttta acatcttcta atttactgtc
    14401 ttctaatgta attatacctt tcatggttcc attatttcta agtatcttag tcaaagctct
    14461 tgtatctatt ccttcaagtc cgataacttt attttgcttt aaatattggt ctatatcaat
    14521 ttcacatcta aaattacttg gagaatcact tttctccctt actatgaatc cctttacttg
    14581 aactttttca gactctgctg atgataaatt tataccataa tttcctacaa gaggataagt
    14641 catagtaact atttgaccat agtaagaagg gtctgttaaa acttcaccat atcctatcat
    14701 tgaagtatta aatactactt ctcctacact ttcttctaaa tatccaaatg cctttcctat
    14761 aaaaactgtt ccatcttcta atattaacct tgctttcata ctacagtacc tcctataatt
    14821 ctctttagac aaactaaaga acattaacta aatataagct tttaaatatc tatatctatt
    14881 tttcactttt acattctagt tttatactta tttacattta ttttttatct ttttctatta
    14941 tactacaaat ttcttaaaaa aaagaagtta tctcaaaagt aaaaactcct agaaaaaata
    15001 acctcttttt tttatttaat aattaaattc ttaaactata caatcaactt aatttctatt
    15061 cagctatatt gtaaactttt aactcatctt tatttaaatg tttcttctta acttctacta
    15121 aagcttttag tgtatccaaa gatgtcatta tttcagtaga aaattcaata gctgttcttc
    15181 ttatcttaaa tccatctctt gtagagtcat ttcctttagt tggtgtattt attactatat
    15241 caacttgttt atttcttata gcatctaata tgtttggtct agattcttct actctattta
    15301 ctatatcagc ctctattcca ttttctctta agctattagc tgttccttct gtagcaacaa
    15361 aagtatatcc taattctttc atatcttttg ctatctctat aaactcatct ttatcatgat
    15421 tatttacagt agcaagtact actcctcttt catcagacat atgtctacct gctgccaaga
    15481 accctttata taaggcttct tctaaatttt ctcctacacc taggacttct cctgtagact
    15541 tcatttcagg tcctagactt acttcaactt tagataattt agacattgag aatactggta
    15601 cttttactga tactaatttt ggctctttgt aaactcctgt accatatcct aaatccttta
    15661 atttagctcc taacatacac ttagtagcca agtcaactat tggtactcca cttactttac
    15721 ttatatacgg aactgttcta ctagctctat ggttaacctc aattatatat aattcatttt
    15781 gaaattctat aaactgtata ttaaccatac caagcacatt aaggtctaaa gctatttttt
    15841 tagtgtattc tactatcttt tcttttattt catcagatat attttgactt ggatacattg
    15901 ttgtactatc tccagagtga actccagctc tttctaaatg ttccattata ccaggtatca
    15961 atatatcttc cttatcacat atagcatcta cttctatttc tctaccagtt agatatttat
    16021 ctataagtac tggattttta tggtctctat caaatgcatc ttgtaggtat tgagataatt
    16081 tttcttcgtt ataagttatt tccatacctt gaccaccaag tacataagat ggtctaacta
    16141 atactggata acctaactca ttagcaactt caataccttc gttaagtgac catacaccct
    16201 ttccttttgg tctatttata tctagtctct ctagtaaatc atcaaatttt tctctatctt
    16261 ctgctgcatc tatatcatca aaatcagttc ccagtatagg tatatttttt tcatgtaaaa
    16321 atttagccaa ttttatagct gtttgtccac caaactgtag tattacacct tctggttttt
    16381 ctttttctat tatacttaaa acttcttctt ctgttaatgg ttcaaaatac agcttatctg
    16441 atgtatcaaa gtctgtactt acagtttcag ggttattgtt tatgattata gtttctatgt
    16501 ccatttttct taaagatttt acacagtgaa ctgaacaata gtcaaattct ataccttgac
    16561 ctattcttat tggaccagaa cctaatacta tcaccttttt cttatcacta acaactacct
    16621 catcatattg ctcataagtt gaataatagt aaggagatat agcatctatc tcacctgcac
    16681 aagtatcaac cattttatat gctggtttga tattgtatag acttctcaat tcatataatt
    16741 tttctggact tatcttcatt aggtcagata tacctttatc agagaatccc tttttcttta
    16801 attcaagaag atagtcttta gttaaatctt caattttcat aacttttaat ttttcttctt
    16861 gctctactat ccatttaaat ttatttataa accatttatc tatacctgta atttgttcta
    16921 tcatttctac tttatagcct cttcttatca tttcagctaa atcaaatagt ctctcatcat
    16981 caggtactac aactcttttc tttaattcct caatgcttct ctcctcagat ggtgtgtgaa
    17041 ctaatgaata ttttcctgtt tcaagtgatc ttatcccttt aagaatagca gcctcaaaat
    17101 tacttcctat actcataatt tcaccagttg ccatcatctt agttcctaat tttctatttg
    17161 cttttttaaa tttatcaaaa ggccattttg gtatttttgc caccacataa tctatagttg
    17221 gttcaaaaca agcataagtt tttttagtta ctgcattttc tatctcatct aaagtataac
    17281 ctaaagctat ttttgatgaa actttagcta ttggatatcc tgtagctttt gatgctaaag
    17341 ctgatgacct actaactctt ggatttatct ctataactgc atattcaaaa ctatgtggat
    17401 taagtgcaaa ttgaacgtta caaccacctt gaacttctac tgcatttaat atatctaaag
    17461 atgctttttt aagcattgca cattctttgt tacttaaagt ttgagttgga gcaacaacta
    17521 tactgtctcc tgtatgaact ccgactgggt ctatattttc catgttacat acaactatac
    17581 aatttccctt tgaatctctt attacctcat attctatttc tttccaacct tttatactct
    17641 tttcaattaa tacttgtcca acaggactta attgtagacc atgtgaaagt atttctctta
    17701 attcttcctc attatcagct atacccccac cagttccacc tagtgtgtat gctggtctta
    17761 caactactgg ataaccaatt gtaagtgcat aatcaagacc tgcttgtaaa tctgtaacta
    17821 tatcactaac tattacaggc tgatttattt ctttcattac ttctctaaat atatctctat
    17881 cttcaccttt tttgatagat tctactgaag tacctattat ttttactcca tatttatcta
    17941 gtatgccttt ttcataaagt tcaactgcta ggtttagccc tgtttgtcca cccatacctg
    18001 caagtaaact gtctggtctt tctttttcta ttattttttc tataaattct attgttaatg
    18061 gttctatata tattttatct gccacttctt tatcagtcat tatagtggct ggattactat
    18121 ttattaatac aacttcaatc ccttcctctt tcaaagactg gcaagcttgt gttcctgaat
    18181 agtcaaactc tgcagcttga cctattatta ttggtcctga ccctaatact aaagttttct
    18241 ttatactatc taatttaggc ataaattttt ctccttatct aaatggtttt tactatctgt
    18301 aaataatact aaatctatat taatgctaag aatttattaa atatacaatc taaatcttct
    18361 tttgttgtgt attctggaat gtactgaaca ctatatattg gtaattcctt atgtctcatt
    18421 ccttctatag tattgtcatt taaatttatg tgagttactt ctgtattttc tggcatttct
    18481 gaaacattat atccatgatt ttgagaagtt ataaatacct ttccagtttc taaacattta
    18541 actgggtgat tacctcctct atgtccaaat acaagctttg aagtctttcc tcctaatgcc
    18601 aatgctaaaa tttgatgtcc taaacctatt cctgtcaatg gctttttgcc tattaatttt
    18661 tttatatttt caactatatc atttatatcc tctggatttc ctgggccatt agataataag
    18721 attaagtcag gatttatatt taaaatttct tctactgtag tcattgctgg tactatagtt
    18781 atatcacagt tacaagcctt aagagactct agtacactat ttttaacacc aaagtctata
    18841 actacaactt tagctccagt accttttata ttttcaattt cttttcttgt aacatttttt
    18901 actgaatctt tgttacaatg ctcttctaat ttagactgaa catcttgtaa tgatacattt
    18961 tctaaagtta taattcctct cttagttcca ttgtttctaa taattttagt taatgctctt
    19021 gtatctattc cttcaagtcc tattacctta ttttgcttta gatatgtgtc tatatccatt
    19081 tcacatctaa aattatttgg gttatcactt ttttctctta caataagtcc ttttacttgc
    19141 accttatttg attcaacatc ttctaaattt attccatagt ttcctataag tggataagtc
    19201 atagtaacta tttgtccata ataagttgca tctgtcaata gttctccata cccagccatt
    19261 gaagtattaa atacaacttc tccaatagct tcctttagat atccaaatgc ttttccttca
    19321 aatactgttc catcttctaa aattaatttc cccttcatgt aagctacctc ccaaatattt
    19381 tatctaagca ttttctattt ctcttacaat attaatagct gattctcttg gattttctgc
    19441 ttttgttata ggtcttccaa caactaaata atgagcacct ttttttattg catctgatgg
    19501 agttacaact cttttttggt ctccaacctc tgctgattta ggtcttattc ctgggcaaac
    19561 tgttataaaa tcagttccac atgcttctac tattttatct acttcttgcg ctgaacaaac
    19621 aaccccatct attcctgatt cttttgccag tttagctctc tttatagcta actcttctgt
    19681 tgtcatatta catcctatgt cctgtaaatc ttggttgctt aatgaagtta aaactgttac
    19741 tccaactact aatggctttt ctatatttaa tctttctgct tcttccctag caattagtgc
    19801 acattgtctc attccttcca tatctgatac atgtattgtc attaaccaaa cattatctct
    19861 cactgcagct ttcactgcac ttttcatagt atttggtata tcatggaatt taaggtctaa
    19921 aaatattttt ttaccttttt ctttaagata atctattgtt ttaccttttg tggcaacgta
    19981 ttgctctagc cctaccttaa atatgtctac actatcttct aatctgtcta ttagttcttt
    20041 agctttatca aattcgtcag tatcaattgc aactatcaat ttttctttac catttatcat
    20101 ttttacatct cctttttaat aaagcctaaa taatcataaa aaaatcctca caccaaaaga
    20161 ggcataagga ttttatctat acataactct attcattagc tatgagatat ctttcccagt
    20221 ctctctggac tagtttaaaa gactttatta tatttaaatt tttattttat actcatcaaa
    20281 aacctctaga agatttacta gaggttatat actaacatta tctataaccc tttagtctct
    20341 ctggactact ttaaggaatt tagctgtaat atttttcttt atttatataa ctttatgtat
    20401 ctataggata atttaaagtt ttatcttttt tataattaaa ccactaattg ctttttatgt
    20461 caataagtct ttgaattatt cttcatcttt atcaaacaat tttttctcaa aatatgattc
    20521 tgcattatat attgcagcaa ctggattatg cccagttact ctatctttga caactaaagt
    20581 tgttatagga gcttccatat atttcatggc taaagtatcg tgaccaacac ataatccaag
    20641 aattacatta aggtcaacct tagcttcatt taaaaataaa gcttgtccta ttggattgca
    20701 cattacctca tctgaacact ttgatattgt ttgagatgct tctagtccaa ttgtactctt
    20761 aggcattgcc ccatttttac atataacaga aaatacttca aacccatgat gttcaaatat
    20821 tttttgcact gtttgcattt ctctttttag tccaatacaa aatgctatgc caatcttttt
    20881 ataaccacac tttttagcaa actctataat ctcttttagt ctagtatatt gacaataccc
    20941 ctctgcttca actaatccag cattataagc tatttttcta ttctcttctt cttcataaag
    21001 ttgctttgct ttttgttgta gttcaatttc tctagttgga catatcttca ttaatttatc
    21061 catatttcca ttagaacagc ctctaagatt acattttcca cacttataca tatccaatat
    21121 ccccttccaa aattttattc atcttgcgat tcttcttttt ctagagttac attttcttct
    21181 tgggtttctt cttctggttt cacatgctcg actacagtta taaaatatga ttttcctatt
    21241 ttattcctta taggaagttt ttcatatgaa acatctattt tattttttat acaagctata
    21301 tcaaaatgtc ttaaggctac acctaagcta accttattca tatcttcaca gtgtttctta
    21361 ggtctctttt caaataaatg aagacctcgt tcatccttat ggaaatacca cggttgagaa
    21421 ttttttacag atggagctct tcttgtcaat tctaagatat ctttccactt tctattccta
    21481 tttatcttat ttatcatatc ttttactggc tttctatcag gtctagcata tctcgtccta
    21541 aacaactctt cattttttgc tggatatcca aatgctatta ttatataagg attttctatt
    21601 ttgttttcat catcttcttc tgtttttatt ccagctagat aatctaaatc atcttcctca
    21661 tcttcttcaa aattcttatt cccaatatct acaaattcca agatatcttc tctctttaag
    21721 tttccttcca accagcaagt agctattccc aatgttgtaa ggcgcaaaac tacaccttct
    21781 gtagcaaatc ctatattctg taaatagtcc tcacctttgt ctgatgtaac tactatataa
    21841 tgaggtgcct ttactttaca ttccttaccc atcaaaaaat gtattatatg acctctatca
    21901 acaacatgtg ctttaatatt taaatcttca tttaagtatt ctaaattttt acaacttctt
    21961 tttatttctt ccatcaatgg cttttttatc ttcttactgg aaaaatttct tacagatttt
    22021 ctataaaaaa tggcttcata aagctccatt tatgccctcc taactgaaat cttctgacgt
    22081 ataaactcta taaatttata taaatacttt atatttttta tagttttatt tcctttttcc
    22141 tcctgcccta ttttgaaatt cataagaata tctactctag tttaatatag cattcaaaag
    22201 gcttaaattc taccttatca atgtctatac attagcataa tttttttatt tgattttcta
    22261 tgctaattta aataacaaca ctagctatta cctttttaat taagctttgc cagaaaaatt
    22321 ttttttcatt tataaatatc tatctcatta ttatattata agtttttttc ggtattcttg
    22381 tatagctttt cactaaaaaa gtatttttat ttctacttta atattattat caacaaaaaa
    22441 catactgctt aaataagttt gttatcctca taatttgttt taattaaaat atttttttta
    22501 tttctcaatc tataaagtat aaatattttg aatatgaccc aaaatctaag taaagttata
    22561 atattttata agttgttcaa tattaaaaac ataagtaatg ttataatact ttataagtta
    22621 ttcaatatta aaaatatagg aggataaata tgaatttaaa tagaaattta gaaaaatacg
    22681 ctgaattagc aataaaagtt ggagttaaca tacagccagg tcaaattcta cttataaagt
    22741 caccaataga atgtgctgat tttgcaagaa atgcacttaa agaagcatat aaatgtggtg
    22801 ctaaaaatgt ttatatagag tggtctgatg aagaaagtac tcttattaaa tacttatatg
    22861 ctccagatga agcatttcat gagttcccca aatggacagc agaacaatat gtagatatag
    22921 caaaagaagg tggggcattt ttatcagtct atgcccaaaa tccagattta ttaaaagatg
    22981 ttgaccctga aagagttgca aattttcaga aggcatctgg aaaagcttta aaagaatggc
    23041 gttcttacac tctaactgat aaatgtaaat ggaatatagt ctctgtgcca acaaaagatt
    23101 gggcaaaaaa agtgtttcca aatttatcag aagataaagc aatagaaaaa ctgtgggatg
    23161 ctatatttaa atgttcaaga gtagatggtc aggaccctat aaaagcatgg gaagaacata
    23221 atgaaaatct taaatctaaa atggactttc taaataaaaa taactttaaa actttaaaat
    23281 ataaatcctc aaaaactgat ttgactctag acttgccaaa gggtcatgtt tggctaagtg
    23341 gagcatccaa agaccctaat ggaataagtt ttaatccgaa tattccaact gaagagattt
    23401 ttggaatgcc acataagttt aaagtaaatg gaactgttta tagtacaaaa cctcttgttt
    23461 atggtggaaa cataattgac aatttcttct taacatttaa agatggtaaa ataatagatt
    23521 tctctgctga aaagggacta gatacactag aaaaacttat tgaaacggat gaaggctctc
    23581 attatttagg agaagtcgct cttgttccat acaactcacc aatatccaat acggatataa
    23641 ttttttataa tactctatat gatgaaaatg cttcttgcca ctttgcaatt ggttctgctt
    23701 ataagacttg tctggaaggt ggaaataatt taaaagatga agaattggat gaacatggtg
    23761 taaatgacag tttaacacat gtagatttta tgattggttc tgctgatatg gatattattg
    23821 gtgagactta tgatggaaag caaatacaga tttttaaaaa tggtaattgg gctttttaac
    23881 atatgatttt aactaaaaag ggctgatgga agttatacca gacttcccag ccctttttaa
    23941 tgttgatgat aaatattttt tatattaatt aatctcatta ttatagatta attcctattg
    24001 ttttaaacat aagtatctaa aacttatatt tatattatat ataaaatatc ctatttagta
    24061 aaacctttat tttaatttat atgctaaaaa tttaactttg ctatttcaac agcagacttt
    24121 gcatcctttg aataataatc agcacccatt tcttcagcgt attcttctgt caaaactgca
    24181 cctcctacaa atacctttgc atttatctca ttatctctca gtgcctgaat tgtatctttc
    24241 atactttgaa cagtcgtagt cataagtgca cttaatccta ctaattttat atttctcttt
    24301 ttagcaactt ccactacttc ttcaataggg acatctttac ctaaatctat aacttcatat
    24361 ccataatttt ctagcattat ttttactata tttttcccaa tatcatggat atcacctttt
    24421 actgtagcaa ctattatttt tcctttggat acattattgc tacttttact taataaagtt
    24481 tcctttattg tatttaatga aacttttacc gtttctgctg attgaatcaa ttgtggtaaa
    24541 aatatatctc cactatcata tctttttcct actttatcaa gagcaggaat taaaatctca
    24601 tctaatatat aattttcatc cttctctttt aaaagattca gtgttacatt ttttgcttca
    24661 tcttttaatc ctctttcaac taaaatatcc aagggcaaat ctttatcaat ctttgtagat
    24721 gaatccgatt tttttgaatt atttatattg ctatattgat ttatatagtt tatacatcct
    24781 ttatctgtgt tatttaatat tttaaaagaa ttgatagctt ccatcatact atattcattt
    24841 ggatttataa ttgctaaatc aagccctgcc cctaaagcta atgttagaaa agaagcatta
    24901 agtgctttcc tatttggaag accaaatgaa atatttgaaa cacctaatat agtttttact
    24961 cctagtgtct ttaccattgt tatagctttt atagtttcta ttgcttcttc ttgttgtgct
    25021 gaaacagtta atgataaaca gtctataaat atatcttttg gttttatccc ataagaaact
    25081 gctctattaa ctatcctttt tgctatttca aatctttctt cagccttttt aggtatcccc
    25141 ttttcatcaa gtgtaagtcc aaccacacat gaaccatact ttttaactat tggtaatata
    25201 ctttctaaag actcctcttt gccatttaca gagtttacta ttgttctacc attataatat
    25261 ctaagtcctt gctctaaggc ctctacattg gaagaatcaa cttgtaaagg tgtatctact
    25321 actgcttgaa tctctcttat taattttggc attagttttt tttcatcaat ctctggaaga
    25381 ccaacattta ctccaagtat ttgtgctcca gcatttactt gctctaaacc taaatttata
    25441 gcataatcaa cattttcatt ttttaatgct tcttgtagag attttctacc tgtgggattc
    25501 aatctttctc caacaactgt aggcgattgt acttccacaa attttgatgg tgaacagact
    25561 acacatttat caattttctc gatacatccc tttgtaacag aatttatatt ctcagatatt
    25621 tcttttataa aacttggatt cgtaccacaa catccaccta ttatagtagc accaacttca
    25681 acgaactttt taaccccttc aaaaaattct tttgcatcga catcatatat agcctctcca
    25741 tcaactatat ttggaagacc tgcatttgct tgaaccataa cagggactgt agctcttgat
    25801 gctatttttt caactattgg taaaagttgt ttaggtccta atgaacaatt aactcctatt
    25861 gcatctacac ctagaccttc tatagtagcc accatacatt ctggcataca tccagtaaaa
    25921 cttcttccac cttcatcaaa tgtcatagta cagaaaatag gtaaatcaga gttttctttt
    25981 gctgctaata cagctacttt agcttcatat aaatccatca tagtctctat tactattaca
    26041 tcaactccag atttaacgcc ttgtagtact tgtcttttaa atatttcata tgctttatca
    26101 aaacttaaag tacccatagg ctctagcatt tctcctatag gaccaatatc taaagctaca
    26161 tatcttggtt tagtattatc tacattttct atagcttttc tagccaccaa aacagcattg
    26221 tcaatcactt cttctacagt atacttacta tctaatttta gttcattaca tccaaaagta
    26281 tttgtaagaa ttacattcga acctgcctct aagtatgctg tatgtatttc tataagttta
    26341 tctggatttt gaagaccaaa tacttctgga ttttcaccta actttaaccc tttttgttga
    26401 agcattgttc ccattgcacc atcaaatatt aatatattat ttttcagata ttgtcttatt
    26461 tccacaagag ttcgctccct ttctatatgt acagtttcta tagtttttac aattttcaca
    26521 cgacttttct ttcttagatt tatttattct tgtattagaa atacctataa tagccactac
    26581 tgactttctc ggtatcataa ttccattaga agtaattgtt agacctattt gtttttgagc
    26641 attcaaaacg tttaatatat ctatattttt ttctattggt aaatctccat atcctgggct
    26701 atatcgcata gttataaatt tatcttgctt taaaaaaacc ttttctaaat tattttgaac
    26761 aatgtcacac acttcttcta ttgctgttgt tccacatgaa tcaattatta gtccttttgt
    26821 taaatttgaa taagagtgtc gtcttatttc tttttctata tctatcccta tagtagctgc
    26881 cataattaca cactcattac attcttttaa aagttcatat acatatttac tctctaatgt
    26941 taaatttgtt ccctctaatt ctacaacatt atctttattt attctaatag gataaatcct
    27001 agataaatat ctaggattta ttttattttt agttatagct acacattcat caatagtata
    27061 tttaaggtct ttatctatct cttgacctct gtattgtaaa tatcttaaaa cttcactttg
    27121 atttatgctt atatcttctc ttccaattaa attattctcc atatatattt aaagaaaatc
    27181 ctttatctaa aacatctttt gcatattcct ttgccccaaa taaagataag tctctgtagt
    27241 ttccacattc tatagctgta gctgctggca ttttatctga ttcaagaact ttttttaatg
    27301 cttcttctaa accaatctta actgttttgg catctacatc tccccatagt attaaataaa
    27361 atcctgttct acatcccatt ggagataagt ctattacacc atctaatgta tctcttaaat
    27421 aagtagctaa aagatgctct aaaccgtgca tagcagcagt tccaaaactt tctacatttg
    27481 gctgtaaaaa tctcaagtca aatttagtta ctttatctcc ctttactcca tcaagtacac
    27541 tacactttct tacaaaagga gcttttactt ttgtatggtc tagcttaaaa ctttctactt
    27601 tttccattat ttaatcctcc attatacttt aaaatttgtt acattaattc taaaaaaaac
    27661 tcttcctatc tattattgat aagaagagta tattatttca agtcatctca tcgttgttag
    27721 caggaccacc tgataaaagg ttggtatagg atcactgagc taactctctc ccctaactct
    27781 tgataagtat cttattaaac tgacttaaat tatatattac ttttttattt ttgtaaacac
    27841 tttattgtta taatatttat atcacataat cgtactttta ttttataact aagctgattg
    27901 tattattggc agttgtactt caaacacagt ttgatattta tcgcttgaaa cagtaatttc
    27961 tccatcatgt aaatttacaa tattttttgt aatcgcaaga cccaatccag aacctccaac
    28021 atttctattt ctcgattttt caactctata aaatctatca aatatatttg gtaaatctaa
    28081 cactggaatt ggttccccat aatttatcac ttttacaacc gccatatctt cctttttctc
    28141 tgtgactata tctacataat gcccatcttt tccatatttc acagcattag ttataagatt
    28201 ttcaaatgct cttaccaatt tatttggatc agcttttaca attaattttt cttcattaaa
    28261 attaattcta ctaaccataa attcttgttt aaaataaatt tcaaattgag atactacttg
    28321 acttaataac tctactaaat ttatttctat tttgtttaat tttattgtat tattttgcat
    28381 tttagtcaat tcaaacaaat catttataag aacatttaaa tttttcgatt tttcatatgc
    28441 aatatcaaca tagtacatca tctgaacttc atctttatac ttaccttctt ctatcactcc
    28501 taaataaccc attatggatg tcaaaggtgt tcttaagtca tgtgacacat ttgttattaa
    28561 atctgttttt gtctgctgag cttgtctctc ttcaatagtt atatttctaa gttgatggac
    28621 tatattattg attttatgtg ctagttcatc aatatcatcc ttttctttat cctttaatat
    28681 atctaaatca tactcaatat ctatactttt ttctaagtta ccttcatcca ttaaatttac
    28741 agcactttta atgatttcta atttttgatt ttttctatta gtctttctta taaaatctac
    28801 aatttgctta tttctcttgt acgatataat ggaataaatt gctaatgatg tgattgtaaa
    28861 taaaaatgga taaaataaat cataccctat aagacttctg aaatctcttg cttgatatac
    28921 aagttgatta actacagaca tatcactatt gacatataat gattttatta tatacatact
    28981 tgttaaaata agcattgaag ttaaagcaca gcttattata agatctaata tgggtcgaaa
    29041 catatgattt aataaacttt tatttttcaa ttttatatcc tactccccat acagtatgaa
    29101 ttattttttc tccatctgta tatttttcaa ttttatccct tattctgctc atatgaacca
    29161 ttacagtatt attagattgg taatatttct ctttccatac cttttcaaat atttcttctg
    29221 tactatatac tctgccttta ttatttgcta gtaaatataa tatatcaaat tctcttgatg
    29281 tcaaaattat ttcatttcca gccactgtta cactatgttt ttccttatct ataattacta
    29341 ctccaacctt tatgacatct ttatcttcat tattagcacc actaaaataa tatgctcttc
    29401 taatcagcga cttcactctc acagtaagtt caagtgggtt aaatggtttt gtcaaatagt
    29461 catctgcacc agtcattatc ccttgtattt tatccatgtc ttctgatttt gcacttaaca
    29521 taagtattgg tatattcaaa ctttctctaa cttttctaca aacttctatc ccatccattt
    29581 taggcatcat tatatccaat atcattaaat gaatattttc cttttttaaa atatctagag
    29641 cctcttttcc atcttcagct ttaattacat tatatccact atttaaaaga tatatctcta
    29701 taaggtctct tatttccttt tcatcatcca caacaagaac tgtattattc atttttacac
    29761 atcctaactt aatctttgta tatagcttta ttcatataaa attatactat attttagtta
    29821 gtgtaatcta taaatttatt aatttctaaa tttccaatat aatacaaatt atttacacat
    29881 ttatattatt tattttgtaa atattcttct aaagttaatc cttttgaata aatttcctca
    29941 gcagcatcct taccaacata tctaatatgc catggttcat atgctgtacc tgttatatct
    30001 tctttacctt caggatatct tattataaat ccaaacttgt gagcattttc tgccaaccat
    30061 ttagcttctt ttgtacttcc aacaaaccat ctttctggat ttgttaaatc aatacaaagt
    30121 ccagtttggt gctcactgta accaggcttt gctacatatg catctgcctt ttctcgcccc
    30181 tgagatttta cccttcttgt ataagtatcc agttgagtat cataagatct gtatgctgaa
    30241 gaccctaaaa actgtattcc ttctgaatta gcttgcttaa ctaaatcttc aactgccttt
    30301 gcaggttctc ctgccatttg tttttcttcc tctgttgctt cttcaacaaa aggtatgttt
    30361 accttagtaa tattttcagg agtatagttt ttgctaattc catttgtctt gtttaccaat
    30421 aatatatttg atatactatt tgagtgattt ttattttgag aatttgcctc aacatcttgt
    30481 tctgaatcat gttgagcagc tactattgga tctttatctt tcttaagtaa tctatcttta
    30541 gatataaata cacttataaa tactactaaa ataatggctc ctattaattt aaattttttc
    30601 ataattaaat cttctccttt ttgttatgtt tgctataatg ttaattttag agtataagac
    30661 ttaagtattt tataagtaaa tcttaaataa atcttaaatt taatattttt aaaggatggt
    30721 gataaaatgt ctatacaaaa acttctaagt aaagctcgta aggctataca agattttgat
    30781 atgatacaag aaaatgataa aattgcggtt ggactttctg gtggaaaaga tagtttaact
    30841 ctacttcata tacttaaaag ttatcaaaga ttttctccac aaaactttga attgatagcc
    30901 ataacattaa atactggagg tgttgataat tctccactag ataaattgtg taaagaaata
    30961 aatgttcctt tttatgaatt tcaaactgac ataaaagaaa ttgtatttga tataagacaa
    31021 gaaaagaatc cttgtgcatt gtgtgctaat ttaagaagag gtgctttgaa cgataatgct
    31081 aaaaaacttg gatgtaataa agtagctctt ggtcatcata aagatgatgc aattgagaca
    31141 tttttaatgt ctatgtttta tgagggtaga gtaaattgtt tttccccaaa gacataccta
    31201 gacagacagg atttaacagt tataagacct atggtttaca tagaagagta tatgactaag
    31261 aaaatttcaa aagattctaa ctatcctatt atcacaaatc cctgccctgc aaatggtcat
    31321 actaggagag agtacataaa aaatcttatt gctaatttaa ataaagagat gcctgatttt
    31381 aaaagaaatg tatttggtgc tttaaataat tctgagaaat tgtttatatg ggataaagag
    31441 aagataaaaa attttaaata ata
//
LOCUS       DACULQ010000023        31273 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.023, whole genome shotgun sequence.
ACCESSION   DACULQ010000023 DACULQ010000000
VERSION     DACULQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.023"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(31..174)
                     /locus_tag="JA154_04525"
     CDS             complement(31..174)
                     /locus_tag="JA154_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HAT7995124.1"
                     /translation="MTISQKDKKKSQNEILYHFETAQIYYFLTYKNHFLIHKKRFCKT
                     LIV"
     gene            411..1694
                     /locus_tag="JA154_04530"
     CDS             411..1694
                     /locus_tag="JA154_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAT7995125.1"
                     /translation="MEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIVENRMLVSP
                     SIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIAEDYMDNTP
                     LSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNEPKVSASND
                     AAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQKTGIGVGYG
                     IKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTAILPIVAYN
                     KKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGISWLMNGTEK
                     QIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVPPLNGIVGS
                     SVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            2022..2561
                     /locus_tag="JA154_04535"
     CDS             2022..2561
                     /locus_tag="JA154_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAT7995126.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            2939..4615
                     /gene="bclA2"
                     /locus_tag="JA154_04540"
     CDS             2939..4615
                     /gene="bclA2"
                     /locus_tag="JA154_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HAT7995127.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGVTGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGENGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVTQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(4817..5572)
                     /locus_tag="JA154_04545"
     CDS             complement(4817..5572)
                     /locus_tag="JA154_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAT7995128.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHDTLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            6012..7001
                     /locus_tag="JA154_04550"
     CDS             6012..7001
                     /locus_tag="JA154_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAT7995129.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(7453..8169)
                     /locus_tag="JA154_04555"
     CDS             complement(7453..8169)
                     /locus_tag="JA154_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAT7995130.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(8393..9142)
                     /locus_tag="JA154_04560"
     CDS             complement(8393..9142)
                     /locus_tag="JA154_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAT7995131.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(9209..10096)
                     /locus_tag="JA154_04565"
     CDS             complement(9209..10096)
                     /locus_tag="JA154_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAT7995132.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(10213..11214)
                     /locus_tag="JA154_04570"
     CDS             complement(10213..11214)
                     /locus_tag="JA154_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAT7995133.1"
                     /translation="MKKVNVAIIGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(11639..12520)
                     /locus_tag="JA154_04575"
     CDS             complement(11639..12520)
                     /locus_tag="JA154_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAT7995134.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGIISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            12843..14036
                     /locus_tag="JA154_04580"
     CDS             12843..14036
                     /locus_tag="JA154_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase"
                     /protein_id="HAT7995135.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            14242..15360
                     /locus_tag="JA154_04585"
     CDS             14242..15360
                     /locus_tag="JA154_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7995136.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            15482..15736
                     /locus_tag="JA154_04590"
     CDS             15482..15736
                     /locus_tag="JA154_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HAT7995137.1"
                     /translation="MIFLKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVG
                     ERGWAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            15723..16469
                     /locus_tag="JA154_04595"
     CDS             15723..16469
                     /locus_tag="JA154_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT7995138.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKSEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            16754..17629
                     /locus_tag="JA154_04600"
     CDS             16754..17629
                     /locus_tag="JA154_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAT7995139.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            18020..18820
                     /locus_tag="JA154_04605"
     CDS             18020..18820
                     /locus_tag="JA154_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HAT7995140.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            19347..21587
                     /locus_tag="JA154_04610"
     CDS             19347..21587
                     /locus_tag="JA154_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAT7995141.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            22001..23536
                     /locus_tag="JA154_04615"
     CDS             22001..23536
                     /locus_tag="JA154_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT7995142.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            23552..24313
                     /locus_tag="JA154_04620"
     CDS             23552..24313
                     /locus_tag="JA154_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7995143.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            24651..25520
                     /locus_tag="JA154_04625"
     CDS             24651..25520
                     /locus_tag="JA154_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7995144.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            25849..26100
                     /locus_tag="JA154_04630"
     CDS             25849..26100
                     /locus_tag="JA154_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995145.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            26212..26991
                     /locus_tag="JA154_04635"
     CDS             26212..26991
                     /locus_tag="JA154_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT7995146.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(27126..27776)
                     /locus_tag="JA154_04640"
     CDS             complement(27126..27776)
                     /locus_tag="JA154_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995147.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(27773..28276)
                     /locus_tag="JA154_04645"
     CDS             complement(27773..28276)
                     /locus_tag="JA154_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT7995148.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(28325..28840)
                     /locus_tag="JA154_04650"
     CDS             complement(28325..28840)
                     /locus_tag="JA154_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAT7995149.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            29183..29644
                     /locus_tag="JA154_04655"
     CDS             29183..29644
                     /locus_tag="JA154_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7995150.1"
                     /translation="MRELLKWVSIINRYNTNYIDRKLADIGINNSQYFYVLHICNNPG
                     ITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTEKSLGCYDEIL
                     KITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENGRKI"
     gene            29652..31031
                     /locus_tag="JA154_04660"
     CDS             29652..31031
                     /locus_tag="JA154_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT7995151.1"
                     /translation="MEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIGQGVGVLGN
                     AATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICLMAVFGISL
                     FILVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLILADGRPKAS
                     MFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKNFQHIKLHK
                     KSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSEIPLACVGI
                     IIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIISVIAFLCFQ
                     LFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGKSVLGLFTS
                     LTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSLTIKQNNLM
                     SNHNKIAEV"
BASE COUNT        11653 a         3859 c         5424 g        10337 t
ORIGIN      
        1 aaattcataa ctttaaagaa agctttaaac ttatactata agtgttttgc agaatctttt
       61 tttatgaatt aaaaaatggt ttttataagt taaaaaataa tatatttgag ccgtctcaaa
      121 atgatataga atctcatttt gagacttttt tttgtctttt tgcgaaattg tcatatgttt
      181 ctccatttta aagaatataa atactctata caaataaaaa aaatacataa atagaggcag
      241 tcataccaat ggttatagaa tataagacta aaaatataaa aagatataaa aaatatggca
      301 tgtattttgc tataaatata gataagaaat tatttaatta attatttgag tactaaatct
      361 aaacttaatt gggaggttta tatataatga gagatataag aaaagaatta gtggaaatgt
      421 taaaggcaga agttaaacca gcagtagggt gtactgaacc agtagcctta gctctagcat
      481 gtgcaaaggc aaaggaatta ttaggagaag aaatagttga aaatcgtatg ttagttagtc
      541 ctagtattta taaaaatggt atgtgcgtag gtataccagg tactgaaaga ttaggattaa
      601 aaattgcagc agctctaggt atagtaggtg gacattctga aaatggatta agtgtgttag
      661 aaactctaac aaaagaggaa gttaaaatag ctgaggatta tatggataat actccactat
      721 caataacacc tgctgataca agagagaaag tatttataga agttgtgtta aaaggaaaaa
      781 atcatattgc taaagtgaga ataagaacta aacatgataa ttttacgttc ttagaaaaag
      841 atggagaagt attattagac aatgaaccaa aagtatcagc ttcaaatgat gcggcagaaa
      901 aagctgaaag tttaatggat acagtaacta tacaagaatt aataaaaaat gtagaaggaa
      961 ttgattttaa agatatagaa tttttactag atggagtaaa aatgaatgaa gagatggctg
     1021 aatatggatt aaaacaaaaa actggtattg gtgtaggata tggaattaaa aagtctatag
     1081 aagaaggtct tttaggtaat gatgttataa attatgcaat gatgttaact gctggagctt
     1141 cagatgcaag aatggcaggg gtaaaaatgc ctgttatgag ttcaaatgga agtggaaatc
     1201 atggattaac agcaattctt ccaatcgttg cttataataa aaaattccca caatctgatg
     1261 aaagactagc aaaagcttta gctatatctc atctagtaac tggttatatt aagaactata
     1321 caggaagatt atctgctgta tgtggatgtg gtgttgctgc ttctactgga gcaactgctg
     1381 gtatctcttg gcttatgaat ggtactgaaa agcaaataga aggagctata gaaaatatga
     1441 tagctgactt aagtggaatg atatgtgacg gtgcgaaagc tggttgtgca ttaaaattat
     1501 cttctgcagc atctgcagct atacaaagtg ctataatagc taagcaagat tgttttgtac
     1561 caccactaaa tggtatagtt ggttcaagtg ttgagcaaag tatacaaaac ttaggtagag
     1621 taagtgacaa aggtatgtct ataacagatg agataatctt aaatgtaatg aatgatatga
     1681 ataaagttga ctagtacttt ataatatgtt ttgtattatg aggtataaca aaaatagcat
     1741 aaataaattt aattagtacc ctctaataaa atatgattat gtataaattt atgtataata
     1801 gaaaaaactt gttaatgcaa gttttttctt tttttgtcta aaatatatag tatgtaattt
     1861 agtgtggata tatgaactac ttaaaatata agatacattg ttaataattg ttgaatgaac
     1921 ataaaaactc aataatattg actaatattg aataagagta ttgaaaaagt gagcaatctc
     1981 ttataaaatt aagaaggatt attacaattg gaggtctttt tatggatata gaaacaaaaa
     2041 aatgggaagt attgtattca gaagaaaaaa ttaaagataa attaagggaa ttaggagcaa
     2101 ttatagaaaa agattataaa gataaaaatc ttatggtagt ttctctatta aaaggtagct
     2161 ttatattttg tgcagactta gttagaaata taaacttacc acttagagtt aattttatga
     2221 ctacatctag ttatggaaac aatgaagaaa gtactggaaa ggttaaagta gtttcagatg
     2281 tgactactga tatagcagga tatgatgttt tggttgtaga cgatattact gattctgcac
     2341 ttactatgga ttttgtgtta aaacatctaa aagctaaaaa tcctgctagt ttaaaatgct
     2401 gtgttctttt ggataaacca agcagaagaa aagtcgattt agtgccagat tattgtggat
     2461 ttgaaataga agataagttt gtggttggat atgggtttga ttttggtgat tattatagaa
     2521 atgttcctta tatattcaat gttactgatg aagatagata agcaattata taattttgtg
     2581 gatgccttaa tatgattttt attaatcgtg ttgaggtttt tttatttaaa aagtaattaa
     2641 agtagtatta aattaatttt atgcaattta attatctatt ttttatagca taataaaaaa
     2701 attttaaata attgatggga aatgagtttt ttaattgtat taataattgt aaaagagtac
     2761 taataattgt aaaagatttt acaaaaaagt aatgaaattt aagaatcaaa gcatatatgt
     2821 tgatataagc aaatagatac attatcacat aagaaattgt cgattcatat aattatatag
     2881 agtaatactc aatatttata gagtattact ctaactttaa aaaaggagga ttaattatat
     2941 gagtgatatt tcaggtccaa gtttatatca agatgtaggt ccaacagggc caacaggtgc
     3001 tactggtcca acaggaccga cggggcctag aggtgtaact ggagcgaccg gagcaaatgg
     3061 aataacagga ccaacaggaa atacaggagc aactggagcg aatggaataa cgggtccaac
     3121 aggaaatatg ggagcgactg gaccaaatgg aacaacaggt tctacaggac caacaggaaa
     3181 tacaggagcg actggagcga atggaataac aggtccgaca gggaatacag gagcaaccgg
     3241 agcaaatgga ataacaggac caacaggaaa caaaggagca accggagcaa atggaataac
     3301 aggttctaca ggaccaacag gaaatacagg agcgactgga gcaaatggaa taacaggtcc
     3361 aacagggaat acaggagcaa caggagcaac aggaccaacc ggactaactg gagcaacagg
     3421 agcaaccgga gcaaatggaa taacaggacc aacaggaaat acaggagcaa ccggagcaaa
     3481 tggagtaaca ggtgctacag gcccaacagg aaatacagga gcaacaggtc caacaggaag
     3541 tataggagcg actggagcaa caggaacaac tggggcaaca ggtccaatag gagcaacagg
     3601 agcaacagga gcagatggag aggtaggtcc aacaggagca gtaggagcaa caggtccaga
     3661 tggtttggta ggtccaacag gcccaacagg cccaaccgga gcaaccggag caaatggttt
     3721 ggtaggccca acaggcccaa ccggagcaac cggagcaaat ggtttggtag gtccaacagg
     3781 agcgacagga gcaacaggag tagctggggc aataggtcca acgggagcag taggagcaac
     3841 aggcccaacg ggagcagatg gagcagtagg tccaacagga gcgacaggag caacaggggc
     3901 aaatggagca acaggcccaa cgggagcagt aggagcaacg ggagcgaatg gagtagcagg
     3961 tccaataggt ccaacaggtc caacgggaga aaatggagta gcaggagcaa caggagcgac
     4021 aggagcaaca ggggcaaatg gagcaacagg cccaacagga gcagtaggag caacgggagc
     4081 aaatggagta gcaggagcga taggaccaac aggcccaacc ggagcaaatg gagcgacagg
     4141 agcaacaggg gcgacaggag caaccggagc aaatggagca acaggtccaa ccggagcgac
     4201 aggagcaaca ggagtgttag cagcaaacaa tgcacaattt acagtgtcct cttcaagttt
     4261 agtgaataat acattagtga catttaattc atcatttata aatggaacta atataacttt
     4321 tccaacaagt agtactataa atcttgcagt tggagggata tacaatgtat ctttcggtat
     4381 acgtgccaca ctttcacttg caggatttat gtcaattact actaacttta atggagtaac
     4441 tcaaaataac tttattgcaa aagcagtaaa tacacttact tcatcagatg taagtgtaag
     4501 tttaagcttt ttagttgatg ctagagcagc agctgttact ttaagtttta catttggttc
     4561 aggcacgaca ggtacttctg cagctggata tgtatcagtt tatagaatac aatagtttat
     4621 atgatgaaaa gattataaaa taataaacaa tatgatttca tataccaata aataagataa
     4681 tatttaagaa aaatgattat atttaaaagg taagcataaa atttataaac ttttagaata
     4741 gaaatctcaa tcaattagat attcaaattg atgaatatga atacctaaat agattgagat
     4801 ttattatgta caaaacttat cctaatatat ctgacatttt ataaagccca gattgcttac
     4861 caattaaaaa tttagctgca actatagagc cgtttgcaaa tacttcatca gattgagaat
     4921 gatggcttaa tgtaagaact tcattatcac ctgcaaatac aacatcatgg tcgctgatta
     4981 tattgccacc tcttatagag tgtattccaa tttcatttgg ttttctttta caatctctac
     5041 catatctacc atgtgtattt tcactttcag gcaagacttc tctaattgca ttagctatca
     5101 tagtagcagt tccactaggg gcgtcttctt ttctattgtg atgtttttct atgatttcaa
     5161 tatcaaaacc ttctaacatc tttgaagcat ccttaactaa ttttattaat atatttactc
     5221 caagagatgt atttgatgat aataatatag gaatgttttt tgatgcttct tctattttat
     5281 ttaagtcatt ttcgtcatat ccagtagtag ctaatactac aggtgtatta ttttttagag
     5341 catatgacaa tattggctct aaagtatcat gatgtgaaaa gtctataatt acatcagttt
     5401 tttcagttac atcagtcatt gttttgtaaa gctttgtatc actatgatga cctttttcat
     5461 cagcagacct agctacacca caaaccaact ctaattcatt atcttttttt acacattctg
     5521 ctaaaactct tcccattgtt ccattatagc cactaattat aactttcaac ataaattcct
     5581 cctatgaaat taataaaaat ttgttatatt tctataagtt tattttatca ctaatataat
     5641 ttatataaaa taatattagt gataataagc ttacaattta tcttacatta aaaaatttaa
     5701 gtggattttt caaaatatag ataaatttct ttttcattgt ttaaatgata aaaatatatt
     5761 tgacttcata ccaaaaagag tgccccatga actaaaaagt taattttgaa ataccatatt
     5821 catatatcat atgatttatt tggaaattaa tattaataca ttaaaataac taaatacatg
     5881 tatgcataaa gatgtataaa aaatatataa acatatagta ttaattttaa attcaacaat
     5941 ttaattgtta aaagtgttat tattaaatta gaaatatctt aacttaatat actgagatgg
     6001 gagtgataga tatgttaaaa gaagaaaaaa cttatcaaga gaaagacgca aaagtattca
     6061 atggagtggc agctttgata atgattttat tatttttagt agtttcaata ggtatcatgg
     6121 tttttgggat agtaaaactt gatagtggat ttactggaat aggttcactt atgttaattt
     6181 tgggacttgt atttatagta gtagatttta tcctattttt tgggctaaaa atgattaatc
     6241 ctaaagaagc tatagttcta gttttgtttg gtaactatta tggaactata aaaaaagaag
     6301 ggtactattg ggtgaatcca ttttgttcag caataaaccc agcagtatca agaattaatg
     6361 tatcgaaatc ttcatctgat gaaaaggtag atatatcaac taattcaagg ggcaaaaaag
     6421 tatctttaaa aacaatgact cttaataatg aaaagcagaa agtcaatgat ttattgggaa
     6481 atccaattat aataggagtg gttgtaattt ggaaggttat agatgcaaca aaggcagtat
     6541 ttaatgtgga taattataat acattcttat ctattcagtg tgattctact attcgcaatg
     6601 tttcaagact ttatccatat gatgtatctg aagatggaga tgaaaaatct cttcgtggta
     6661 gtagtcagga aatagcagat agattaaaag atgaattaca atcaagggtt gacattgcag
     6721 gaattgaagt gtgtgaagtg agaattactc atctttctta tgcaccagag attgcagcag
     6781 caatgttaca acgtcaacaa gcagaggcta taatagcagc acgtaaaaag attgttgagg
     6841 gggctgtaag tatggtagag atggcattga ctaatttaag tgaaaataat gttgtgactt
     6901 tggacgaaga acgtaaagca gctatggtaa gtaatctgtt agttgtgctt tgtggcaata
     6961 aagatgcaca accagtggta aacagcagtt ctatttatta aattataaaa agtttaatag
     7021 tttattttat tgatatatag aaaatttagt agttcatttt attaaattat ataaagatta
     7081 atggtttgct ttattgaatt ataaaaagtt taataattta ttctattgat atatagaaaa
     7141 tttagtagtt cattttatta aattatataa agattaatgg ttttcacttt attaaattat
     7201 aaaaagttta atagtttatt acattgatat attgaaaaat tgaaggaaat aaaaatactt
     7261 gtcagcaaga aataaaaatg atataagaat gaataagaga attgtaaaaa aataaaatga
     7321 ttataaataa aagataaaaa tattgtaaaa aagagggtgc ctctggtgaa ttaaattcac
     7381 acagagacac ctttttaatt aattttttaa attagtatat tattgtttaa ttaatagcat
     7441 agtactattt aattaatcta aatttctaag gtcttccata agcttagttt tgtcagccgt
     7501 tttttcatct ttcattttta taactttagc aggagaacca gcaacaacgg caccagcttc
     7561 tacatcagta gttacaacag caccagctgc aacaacagca cctttaccga ttctaacacc
     7621 ttcaagaata actgcattag ctcctattaa aacatcatct tcaactataa caggagtagc
     7681 agaaggtggc tctaaaacac cagcaacaac agcaccagca cctaagtgaa catttttacc
     7741 aagagttcct ctagctccaa taacagcatt catatctacc atagaacctt caccaataac
     7801 agctcctata tttataacag ctcccatcat tacaacagca tttttttcta tagtaaccat
     7861 gtctcttatt attgcacctg gctcaattct tgcatgttca cttaaagtat tttttagagg
     7921 aatagcagaa tttcttctat catattctac atgagtatct gttatatcat ttccatgttt
     7981 atctaatact tccattatat cctcatagtc acctacaaat atataagaac cattacttcc
     8041 aaatacctta aatttatcag ttgattggaa gtgtaaagag ttagtattta catatacctt
     8101 aacaggtgtt ttctttttaa cttcttttat ataccttgct atttcataag catcgtttaa
     8161 atttaccatt tgttaattct ccttgtattt cattatattt cttttattat atacttattt
     8221 gtcaatttta gtatatactt acttcttatt atttatattt tattttagtt agcttttatt
     8281 gtatagacat agtattaaat aaatttatta tatatgtttg atttgtattg catattatta
     8341 tatatatatt gtatatgttt aatttatatt gtgtattatt atatttatac tattatccca
     8401 acatgctgtc catattataa aatcctggtt tttgcttaac taagtatttg gcagcagcta
     8461 tagaaccact tgcaaagata ccacgagatt gagcttgatg gcttattgta agaacctcat
     8521 tgtctccagc aaatagagca tcatgttcgc caacaatatt tccacctctt atagcatgaa
     8581 taccaacttc atttggttgt ctttttgcac tacgaccata tctaccataa ttattttcaa
     8641 cgtttggcaa aacctctttt atagcgttag caatcataac agctgtgcca cttggggcat
     8701 ctacttttct gttgtgatgt ttttctatta tttctatatc aaatccttct agtaatttag
     8761 ctgcatcttt aaccaatttt actaatacat ttacaccaag agacatatta tatgattgga
     8821 atacaggaat aactttagcc gcttcacgta ttttgttcat ttcgtcatca ttatatccag
     8881 tagtagcaat tactaaaggt gttttagtct ttaaaacgta agataagata ttatctaaat
     8941 tagagtgatg agaaaaatct ataactacat cagcttcctc aactatagta ctcatatctt
     9001 cataaatttt aaggtcgcct tcatagctat ccagacctct agctgcaaca caaaccaatt
     9061 ctaattcatt atcttctctt atagtatcgg ctaaaatctt acccattttt ccatcataac
     9121 cagttgctat aacttttaac ataatttcct cctatgatta agggctaact tttgaccaaa
     9181 cttacttttt accatgcatt aatctttact atgcattaac ttttagtcca aagttaacta
     9241 attctttctt taatacttct aaatttgctg ggtccatttc tgctagaggt aatcttaaat
     9301 ctccaacatt gaatcccaat aaattcattg cagtcttaac tggtatagga tttacttcta
     9361 taaataaagc tgcgattaga gcatccatac caagttgtaa ttttctagaa ccttcaatat
     9421 cgccttcaaa gaatttagct accaaatcgt gagtttcttg agggcaaaca ttagcaagta
     9481 ctgatataac tccttgacct cctaaagata ataaaggaag tatagtatca tcattaccag
     9541 agtagatagc aaagtcatct ggaactaaac tagctatttc tgcaacctgt cctaggtctc
     9601 cactagcttc ttttacagca actatgttat caattttagc aagttcaact ataaggctag
     9661 gacttatatt tacacaagtt cttccaggga cattgtaaag tataattgga agtttaacac
     9721 tgttagctat tgtttcaaaa tgacgcttaa gtccagcttt attagcttta ttgtaataag
     9781 gtgttattat taatagagca tctactccta atttttcagc ttcttgactt aagttaactg
     9841 agtgcatagt atcattagaa ccacttccag ctataacagg aactctttta ttaacttttt
     9901 caacaacata cttaatagta gcaagttgtt cttcatcagt catagtatta gcttcaccag
     9961 tagtaccaca agatactata gcatcagtac cattagctat ttggtattca actaattcac
    10021 ctaatttgtc aaaatcaact ttgttatcct ttgtaaatgg tgtaactaga gcaacagcag
    10081 aacctttaaa tatcatataa aatcaatccc ctctttttta tttttgtgtt ttatattaca
    10141 gttacttcta atgcttgttt ttgagttact attaatattt tacaaaatta tatatttata
    10201 caagtttgta tattatacta aatcgtatga taaagccatt tcagctattt gaattgcgtt
    10261 agttgcagca ccttttctta tattatcagc aacaacccaa agatttacac cattatcaac
    10321 actaaaatct cttcttattc ttccaacaaa tacttcatct ctatcatcaa attctacagc
    10381 agttggatat tcgtttttag caggattatc aactactaca acacctggag cttcttcaag
    10441 tgtttttact aaatcatcta actcaaatgg attgttaaac tcaacattta tagattcact
    10501 atgaccattt tttactggaa ctcttacagt agttgcagtt atttttaaat cgtagttatc
    10561 aagtattttc atagtttcat ttaccatttt catttcttct ttagtgtatc cattttcagt
    10621 gaatgaatct atatgaggaa gacagttgta agagatttga tgagggtaga atccagtagg
    10681 attaccgttc attcctttct ctaaatcttc aacaccttta acaccagaac cagatacagc
    10741 ttggtaagta gagtaaacaa ctctcttaat tttgtattta tcatcaagag ctttaagggg
    10801 aaccatagct tgtatagtag agcagttagg attagctatt atacccttat gattttttat
    10861 ggcttctgga ttaacctcag gaactactaa aggcacatct ggattcattc tccattgact
    10921 agagttatct actactaaga ttccatttga agcagctatt ggagcatact tttcactaat
    10981 acttccacca gcagagaata atgctatttg tatgcctcta tcagtaaatg aagtttcctt
    11041 taattcttct actatatatt ctttaccaca aaacattact ttagaaccag ctgatttaga
    11101 agaagagaat aaaaatagat tttctattgg aaaatttctt tcttctaata cttttaaaaa
    11161 tgttctacct accattccag tagcaccaat tattgctaca tttacttttt tcatataaat
    11221 tcaccccaat tttaaattat taaaacatta ttttatttta gttatacaag taagatatgt
    11281 taatattttt atatatccaa aaactttaat attatattaa caactttaat tatatacaaa
    11341 aaacgtacaa aggtatactt tgtacgtcta atttatcact caaagtaaac cctgtagctc
    11401 atcacctaca aaaataagtg acagtattac acatgttatg tgcaatccca gaaaatatta
    11461 ttgcaagaaa tattttcttc ggctgtaaat cctttttcca atcattaaca cctgccagct
    11521 actattggat actcttagtt acagctacct ctaccctagg ttaataggat attaaaattt
    11581 gattattcta ataataatac atatttaaga aaaaatcaac ttatttatat aaatattgct
    11641 aaaatttaaa tccaaaattt gtcaattctt gttttaacac attaagatta gtttcttcca
    11701 tttcagctag aggaagtctt aaatcaccaa cattaaaacc taaaatgttc atggcagttt
    11761 taacaggaac tggatttact tctataaata aagcagcaat taaagcatcc atatcaagtt
    11821 gtaatttttt tgagccttca atatcacctt caaagaattt agttactaaa tcatgtgttt
    11881 ctttaggaca tatgttagca agtacagaga taataccact tccacctaat gataatagtg
    11941 gtagtatagt atcatcatta ccggagtata tagcaaagtc tttaggtact aatttagcta
    12001 tttctgcaac ttgagctaag tctccacttg cttccttcac agcaactata ttatctattt
    12061 tagcaagttc agccacaaca gatggcttta tattcacttt agttcttcct ggtacgttgt
    12121 ataaaataat tggcaactta acgctatttg ctattgtttc aaagtgaagt ttaagacctt
    12181 tctcattagt cttattatag taaggagtta ttattaaaag accatccacc cctaattttt
    12241 cagcttcttg actcatatga acagacaaca tagtattgtt tgaaccagta cctgctataa
    12301 caggtattct cttattaact ttttcaactg tatattttat tacagcaaaa atttcagatt
    12361 cactcatagt agtcgcctca cctgttgttc cacaaacaac tagtgcatct gtgccattct
    12421 caatgtgata ttcgattaat tcacctaatt tttcaaagtt gacattatta tcctctgtaa
    12481 atggggtaac taaagcaaca gcagaacctt taaataacat ataatcaaat ctcctttttt
    12541 atctaaattt aattttttaa gtacaataat aattatagta caaattaatt aattttaata
    12601 ggtatagtta atatcttaac tgtttaaata agatatttat attatattaa tattagaaaa
    12661 attttaaata aatgttttat tctcaaaata tgcatattaa tttaataaaa tataccaaaa
    12721 tggaaataaa aaataaattt tatgagaatt taataactaa taattaatat atttaggtta
    12781 taataaatat gtgcgtaaaa tctaacaata agcaataata ccgtaaattt aggaggaata
    12841 atatggatac tttaaaggaa ctttttaaaa taggaagtgg accttcaagt tctcatacta
    12901 tgggaccaca aagagcagca gagagattta agaatgaaaa cccagatgca gaaagtttta
    12961 gagcaatatt atatggtagt ttggcagcta cagggaaagg tcacttaact gactatataa
    13021 ttgaaaaaac tatagcacct aaaaaagtcg aaattgtttg ggaagaagat attataaaag
    13081 attttcaccc aaatgggatg aagtttgaag ctttagataa agataaaaat gtaacagctg
    13141 aatggactgt atattctgta ggtggaggaa ctatagcaga agaaggtcaa agaaacagta
    13201 aaagcaatag tatatatcac ttagatacta tggatgagat agtaaaatgg tgcaaagaaa
    13261 ataataagac tttggtagat tttgtgttgg aatgtgaacc aaaagacata aaagattata
    13321 taaaaactat aaaagatgct atgagaaaat ctattgatga tggactaagt acagatgaaa
    13381 taataccagg caagttatta ttaaaaagaa gagcaagtac attttataat gcatataaaa
    13441 aagataagag cttctcaaca cttgtatatg catatgcact agcagcttca gagcaaaacg
    13501 cttctggtaa tataatagtt acagcaccaa cttgtggttc tgctggagta ataccaggaa
    13561 tattctttgc aatgcaggat ttctataatt atgatgacga aaaaataatt gaagcactac
    13621 ttgtagctgg aataataggt aatataataa aaactaacgc ttcaatatct ggagcagagg
    13681 tagggtgtca aggtgaagtt ggagccgctt gttctatggc agcagcagca gtagcatact
    13741 taaaaggtgg aactatagac catattgaat atgcggcaga aatagcactt gaacatcatt
    13801 taggaatgac ttgtgaccct gtatatggat atgttcaaat tccatgtata gaaagaaatg
    13861 caatggcagc tcaaagagct tatgatgcag ctaattatgc attattaact gatggctcac
    13921 attctgtctc cttagaccaa gttgttgaaa caatgaaaga aactggtata gatatgatgg
    13981 ataagtacaa agaaacagct aagggtggac ttgctaaaca tttcttctct tgctaaaaat
    14041 aaaattttcc aataataatt cctctgtttt gatttatgaa acaggggaat tattattaat
    14101 ggtgaattat ttaataatgc aaaatatttt tatattcaat ttagcttttt atttttaatt
    14161 agtataacat gctatataat attttaatta taaagaaata tagaaatatt ttttaataaa
    14221 ttagaaattg gaggagatta catgagagag gcatcatatt tacaagaaca actttataaa
    14281 cataggaagt cattaaatcc tattgcagag attgggagaa aagagtataa gacttcaaaa
    14341 tatataagag attatcttga taaattagga attgagtatg aagtttattt agaaacaggt
    14401 atagtaggtg taatagaagg taaaagacct aaaaaagata ttgcttttag agctgacata
    14461 gatggtcttc atagtgaaga aggggtaaaa catttatgtg gtcatgatgg acatgctact
    14521 atcttacttg gattaattga gtatttaaat gataataaag aagacttaaa tgataatata
    14581 gtatttatat tccaaccggc agaagaaggt cctgggggag caaaagcttt aatagatgaa
    14641 ggagtgctta gaaaatacaa tattgatgaa atatatgggc ttcatatata tccagagtta
    14701 ccagaaggtt atgtaggtac aagagagggc tattttatgg ctcaaatagg agatattgac
    14761 atagaaatag tgtctaaaag tggacatggg gctatgccac aaaatggtat tgatggtata
    14821 gtgatagctt caaactttgt aagctcactt caaactatag tgtctagaaa tataagccca
    14881 atagacaatg cagtgttgac tattggcaga attgaagggg gagctagaag aaatataata
    14941 gctgaaaata taaagatgga aggtactatg agatgtttca gcccagaagt gtatgagaaa
    15001 atgaagctta gagttagaga acttgctaga gggtttgaat tggcttataa ctgtaaggtt
    15061 aatataaata ttatagatga ttatatcgct gttaaaaatg acaaggattt gtatcaagag
    15121 tttgtagagg ctattggaag tgatactgtt gtagagttag agccacttat gatatcagaa
    15181 gatttttctt attatcaaaa agaagttcca ggattatttt ttatgttggg ttcaagaaac
    15241 gaagaaaaag gatttgtaaa tggtcttcat aatataaact ttaattttga tgaaaagata
    15301 tgtgtaaatg cattgaatgt ttattcaaaa ttattaaaat acaaagaagc aatagattag
    15361 gaagataagt tataaagatt aaaaagcata aatacctaaa tttaaaaaaa gtgtattaat
    15421 aaatacccct tacatatttg tataaattta ttttaattga caaaaataca aataagaagg
    15481 ggtgattttt ttgaaagaaa aagtacaaag agaaaagaag ataactaatg gcaagaatac
    15541 taaaatattg acaaatgatg atataatgaa atatgagata gcctcagaac taggtcttat
    15601 ggataaagtt ggtgaacgtg gatgggcagg acttacagct aaggaagcag gaagaattgg
    15661 aggtatgctt acatcacgca aaaagaggct taaaaagatt gtggaggagc agaataaaaa
    15721 ggatgagtca atatagtgat tttgcgtttg tatatgatga acttatgaat gaagttgatt
    15781 ataatggttg ggttaaatat atagaagata taatagatag tgaaggtgta aaagtaaaaa
    15841 atatattgga attggcatgt ggcacgggta atcttacaat tccattgact aaaaaaaatt
    15901 acgatatagc tggtatagat atatctgatg agatgcttag tgtagcaaga gaaaagtcag
    15961 aaaaagaagg agtagaatta gttttacttc aacaagatat atcagagctt gattttgaaa
    16021 tatctgattt ggactgtgta ctttgtgctt gtgatggatt caattatata acttatgatg
    16081 atgatttgga aaatgtattt tcaaagacat atgaattatt aaagaaagat ggaatattca
    16141 tatttgatat aagttctttt tataaattag ccaacatatt gggaaataac atgtatggag
    16201 aaaatagaga agatatagcg tatatgtggc aaaattactt tgatgaagaa gagaatctaa
    16261 ttgaaatgga acttacattc tttattaaag atgaatgtgg aaaatttgaa agatttgaag
    16321 agatacacca acaaagagca tatactgaag atgagatagt tgatatgctt gaaaaatctg
    16381 gatttaagga tataaaatat tatggggatt ttacatttga caaacctagt gaagaaagtc
    16441 aaagaatctt ctttgtatgt aaaaaataag atatttttga ctatataaga taattattaa
    16501 aaataaccat gatataaaaa tatagaaaat gcagttttat agattaaaag taaagtgatg
    16561 atactttatg acttttattt tttaataatt ataaagttct atagataaaa attaaaatat
    16621 aataactatt gcttagtaaa attaaaataa aatgggtttt gcccaaggtt aagatagata
    16681 agtatttata gattaaaatt aaggtagtaa tataataaaa ataagacaat taacgggaca
    16741 tggaggaata ttaatgagag attatgtact aagagcgaca tcaggaaatg gtcaagttag
    16801 agcatttgtc gcaactacta gaaatacagt agaagaagct agaagattac atgaaactac
    16861 aaaagtagca acagcagcac ttggaagaac attaacagcc acatctataa tgggattaat
    16921 gatgaaaaat gatagtgata aattgactgt aataataaaa ggtggaggtc caattggaac
    16981 tattatcgcc acatcagact ctaagggaat ggtaaaagga tatgtaggaa atccacaagt
    17041 agaggttgaa gactatccaa atggaaagtt aaatgtagca gcagcagtag gtacagaagg
    17101 tgttgtaaaa gttataaaag acttaggtct tagagaacca tataatggga cttatccatt
    17161 ggtaagtgga gaaattgcag aagactttac ttattatttt gctgtatcag agcagactcc
    17221 atcagtagta gctttaggtg ttcttacaaa agaagatgaa gtagagtttg ctggaggatt
    17281 tatagttcaa ttaatgccag atgcagaaga agagactatt gctaagttag aggaaaatgt
    17341 agctaaatta ccttctataa ctaacatgtt aaaagaagga aaatcaccag aggatatatt
    17401 aaatattgtt ttggatggtt tggaacctaa aatacttgat acctgtgaag ttgggtttat
    17461 gtgtgaatgt tcaaaagaaa gagtaaaaac tgctttggtt gcaattggta aaaaatcatt
    17521 ggctcaaata atagaagaag ataaaaaagc agaagttggt tgccagttct gtaataagaa
    17581 atatatgtat agtgaagaag agttgcttga aattttaaag gaaatgtaga ctataaaaat
    17641 agttgtattg atataagtaa tgttatagtg atatagtaaa gtcgtattga tatagtaatt
    17701 aaagtataat ttattaataa tagacatatt tatattaaaa aataagttta ataaattttt
    17761 aagcttaata aattttaaga attaagaagc tttctggctg taatgtttgc aatgttgcag
    17821 ttagaaggct tttttatttt attatagttc gtgtcaaaga ttgtatgaaa caataaaata
    17881 ttgtaaaata atttgtatat ttagacctga ttggcacaaa ggtttataag gtatttgagt
    17941 tgattatgcg aaattctatg aagtagtaac cctgtgactt taatcatggg aggttcagaa
    18001 gaaggagaaa attttaaata tggaaagtaa aacgaagtac ataggaataa gtatattaat
    18061 attattgatg atatttttaa ttgcaaaaaa ttttaacgga aaacttgaaa tacctattcc
    18121 tgagagtaat agtagaaaat ttaaatcagc agcagtgttt tatcctcaac atcaagatga
    18181 tgaggtttta tggggaggaa gtgccattgt agatgctata aaacagtgtg gtgttgataa
    18241 tgtatatgtg gtattagtat cagatggttc tggagttaac gttttcaaag ctaacacaaa
    18301 atttagaaat ttaactagga agcaaaagga agaacttaga aataacgagt ttaaatcagc
    18361 tttgagagag cttggagtga aacctcaaaa tgtgattata cttgcagata tagataaaaa
    18421 ggaaggcaca cattatgaat taatggaaaa gactatattg gattttgaac ataagtttaa
    18481 aggcaatgtg acccatatag cccatcacta taagtatgat gaccatataa tgcatagaaa
    18541 gaatggggaa gttttaaaga aacttaaacg tgaaggtaag ataaaagatg acttgtattt
    18601 tgttaagcca aaatatgtaa agtatatacc aagaaatgaa agagctatct ataaagcaat
    18661 ggattttgat gactacaata aggttaagag tgcttgttat gaatataagg ttgtaaattc
    18721 aaagcgtaaa atgtatggta taggatatat ttcggcacat agttattttg aacatcttct
    18781 aaatgaccct gaacttacgt ctgtattaag tgaagattaa tttcttataa gtagtaagtt
    18841 atgtttgata agttgattgg aattttctat agttttgaag gtattggaaa tttagtttta
    18901 tttaaagatt taaagataga ggtttttagt gtttaaaatt ttaaagttaa gttgaataat
    18961 tgtatagaat tatttgtgaa agaagaaaca gtgtaaaaca tagtatctta tttatggttt
    19021 taaaatcata ggcaagatac tttttttatg taactaatac cattttgaga ttattaactt
    19081 aagaatttta tataatttaa cactcatata taatgatgat atttaggtag aatagatgca
    19141 attacgctta aataaaataa ctgtattttt tataaaaatc aaaccctaca ctatactacc
    19201 agataaaatc tacatgctat aatttgacct gtgtttataa aaaaaggagt aatctaataa
    19261 aatagtattt cacttatgta taactaacaa ctattattac gattttctct aaataattta
    19321 aagaaaaata atgcaggagg taaaatatga acaatgaatt tataatttta aaaggttgca
    19381 aagagaacaa tttgaacaat atttccttaa aaataccaaa aagaaaaata accatattca
    19441 caggggtgtc gggttctgga aagtcatcta ttgtatttga gacaattgca aaagaatcac
    19501 aacgccaact aaatgaaagg tttagtacat ttgtaagaag ttttttacct aagtatggag
    19561 aggttaaagc tgattgtata gaaaatttgt caacaccaat tatcatagac caaagtagac
    19621 ttggtggaaa ttcaaggtca accctaggta ctattacaga tgtaaattcc tttttaagag
    19681 ctttatattc aaggtttggt agtgagtata taggtaatgc taatatgttt tcttttaata
    19741 atattgatgg tatgtgccca cactgtcatg ggcttggaaa aaagctagtt cctaatatga
    19801 accaaattct tgatatgaat aaatcattaa atgaaggtgc tatattgctt tcagggtttg
    19861 gagtgggttc ttggcattgg aatatattcg ctgaatcagg attttttgat attaataaga
    19921 ggatatgtga ttattcagaa gtagaattgg aaaagttttt gcatggtaaa gcagaaaaga
    19981 ttaagataga aaatgctggt caaactaaca tgacatatga gggattaatg gttaaattta
    20041 atcgcctata cttaagtaga gagggagaaa tatctgaagc tactaagaaa aaattgagta
    20101 aacttcttat agaagataga tgtcctatat gtgatggaag aagacttaat gaaagagtat
    20161 ataggtcttt aattaatggt tataatatag cagatttaac tagtatgcaa attgatgaat
    20221 tggctgaagt tattaaaagt atagatgagc cagaagcaca acctttaatt aaaggaataa
    20281 tagaaaagtt aaacagtatc atagatatag gtcttggata tttaacactg gatagagaga
    20341 catcttctct atcaggaggg gaatctcagc gtataaagat ggttaagtat ttaaatagta
    20401 atcttgttga tttaatgtat atttttgatg aaccaagtgt aggacttcat ccaaaagatg
    20461 tgtataaatt aaataatctt ttaaaaaaat tgcgtgataa agggaataca attatagttg
    20521 ttgaacatga cccagatgtt attaagattg cagaccatat tgtagatgta ggaccaaagg
    20581 caggtaagta tggtggagaa gttgtgtatg aaggcagtta tgaaaatcta cttacaagtg
    20641 gtactttgac agggaatgct ttaagtaaat ttcttcctat taaagaaagt gtaagagaac
    20701 atagtggata tttagaagtt aaaaattgta ataaaaacaa tttaaaaaat gtatcgatta
    20761 agatacctaa aggtgtattg actctagtta ctggagtagc aggagcaggt aaaagtacgt
    20821 tgattaagga tgaattttta aagcaaaatc ctagtgctgt acttatagac caaagccctg
    20881 tttcagcaaa ttcaaggtcg agtttggcaa catatagtgg aattatgaac aacataagaa
    20941 gcatattttc aaaaacaaat ggcgtaaatg cttctttatt cagttcaaat tcagaaggag
    21001 cttgtgataa ctgtaagggt tctggaatag tagaaatgaa tcttgctttt atggagagta
    21061 taaagagtac ctgcaatgtt tgtgaaggta aaaaatttaa aaaagaagtt ttagaatata
    21121 ggtttcaagg gaagaatata attgaagttc ttgaaatgtc tgttttagag gcaattgagt
    21181 tttttaattt aaaacagatt aaaacgaaat tacaatgtat tgaaaaaatg ggtattggat
    21241 accttacttt aggacaaact cttgatactt tgtctggagg agagtgtcaa agattaaagc
    21301 ttgctagtga attaaataag gaaagttcag tgtatatatt agatgaacca acaacaggtc
    21361 ttcatatggc tgatattgag aattttataa gtataattga agagattgta gataatggaa
    21421 atacggtcat aataattgaa cacaatatag atgtaattaa aagagctgat tggattattg
    21481 agctgggacc tgaaggagga acaaaaggcg ggagagtaat atttgaaggt attccaaaac
    21541 aattatgtaa caataaaata tcattaacag caaaatatat agtataataa ataaaatata
    21601 ataaaattat aatgatattt taataaatat aatgggtata taaaatgata attaaactta
    21661 acagtaataa ataatgtgag aaatattcaa atttaattaa ataaagtgtc agaaatttgt
    21721 ttaaatttta gtattttaac ggttctgacc ttttttctta tttttatgga gaatatcgag
    21781 gatggagtct ctaatagtta cttttttgtg tctacttgca agtttgatta aaaaatggct
    21841 atacacactt gtgacttcag tcatgggagg ttcagattat aatcttaata tataatttgt
    21901 gaaataattt gatgacgatt tgctgatgta taaacaataa agattaacta cattaaaatt
    21961 taaatcttgt taagtaaaac ttataaaagg agagagatgg atgataaatg atattctaac
    22021 cttattatct gaaagaagtg atttttttat aaagctatcc atagaacatt tgatgatttc
    22081 tattatttct ataataattg cgattgtttt gggtggtata attggtatta ttgtaagtga
    22141 atttcataat tcttctaaac ctatattggg aactatcaat tttctataca ccattccatc
    22201 tatttctatg ctaggatttc tgataccatt ttccggaatt ggaaatacta ctgctgttat
    22261 tgcacttacc atttatgcat tattgccaat ggttagaaat acatatactg gaattactaa
    22321 tgtggataaa aatattcttg aagcagctag gggaatggga agtacagatt ttcagatatt
    22381 gtataaaata aaacttccgt tagccctacc tgttattatg tctggtatta gaaatatggt
    22441 gattatgaca attgcattag ctggtatcgc ttcatttata ggtgctggag gtctaggtgt
    22501 agccatatat agaggaatca cgacaaataa cacaataatg acaattactg gaagtatact
    22561 tattgctcta ttagctttaa tatttgattt tattttaggg ctggtagaaa aattttttca
    22621 acgaaaaaga aaaaagaaaa ataggaaaaa agtttttgcc attctaggaa gccttattct
    22681 tatagcaact atctttacgt cttatttatt taatccttca aagactcaaa ctattcatat
    22741 tgctactaaa cctatgactg aacaatatat tcttggtgag atgttggata ttttaattga
    22801 aaaaaacaca ggtctaaacg tggaaattac acaaggtgta ggagggggaa catctaatat
    22861 ccagccagct atggagaatg gagaatttga catttatcca gagtatacag gtacaggctg
    22921 gaatatggta cttaagaaaa atgaattata tactgaaagt atgtttagta aactacagtc
    22981 ggaatacaaa actaaactta aaatgcaatg gactggtatg tatggttttt ctaatagcta
    23041 tgcattagca gttagaaaag aagttgcaga taaatacaat ttaaaaaata taacggactt
    23101 aaaagatgta tcttctaaat tgacatttgg agctgaatat gacttttatg agagagagga
    23161 tggatatact ccattgtgta atacatatgg actgaacttt aaagatacag ttgatttaga
    23221 tattggatta aaataccaag ctatcaatga aggtaagatt gacgtaatgc ctatatttac
    23281 aactgatggt cagtatagtg tttcagatgt tgtagttcta aaagatgata aaaatttcta
    23341 cccatcttat caatgtggaa atgttgttcg tttggaagta ttagaggaaa atccagaact
    23401 atcaaaggta tttaagatgg ttggagatat tttgactgat aaagaaatgg ctaaaatgaa
    23461 ttatgacgta gaaaacaaca atttagaacc tagagaagtt gctcgtgagt ttctgaataa
    23521 gaaaggtctt ttataaattg gaggaaatat aatgacacct attatacaat ttaaaaatat
    23581 taaaaaacaa tataatgata aaactataat agataatctt aatttagata tagaaaaagg
    23641 agagtttttg actgtaattg gttcgtctgg ttctggtaag acaacactct taaaaatgat
    23701 taatggtctg attttgccag atggaggaaa tattttgata aaccaaacag atatcaaaaa
    23761 cgaggactta attaaattaa gaagaagaat aggatattgt gtacagggaa gtgttctttt
    23821 tcctcatatg actgttgaag aaaatatttc atatgtacca aatctcctaa gtaaaaagaa
    23881 taaattagaa gtaaaaagtg ctgtaaacaa gtggatggaa atagtgggat tgccaaatga
    23941 tatgaaagtg cgttacccat cagaattgtc aggtggtcag caacaacgtg tagggattgc
    24001 aagagcttta gcttcttctc cagaaatttt attaatggat gaaccttttg gtgcagttga
    24061 tgagattaca cgaaagcaat tacaaaaaga gataaaagaa attcataaaa aaacaggagt
    24121 tacaattatc tttattacgc atgatattta tgaagctttg attttgggaa caaagacttt
    24181 ggtattaaat catggagttg ttcaacagta tgatactcct gagaatattt taaatactcc
    24241 tgctaaccaa tttgtagacc agttattgaa tataagaaaa tctataatag atgatgataa
    24301 tttaaaagaa taaatttaaa tttaaaatca aattgttgtg ataaaaacta ttgatttaga
    24361 gctaacttca agttgtatac ttgaagtgga aaagttagat taaggtatag aagggcattt
    24421 agcagttggc tgtagacgag gtaactgctg aaggtgcttt caataaatgg ctagaacctg
    24481 tatctgttga agattataat aaattgaaat aagaagctca atatacagag tagataggca
    24541 cttcaaatag aagtgtcttt tgtgtgccat aaaataaatc aaaatattag caaagaatgt
    24601 gaaaaacaaa ataaaataat atgctatctt taaaatagga gggatgctat atggataaca
    24661 atttgattac cattgaaaaa actttagatt atatagaaaa acatctttta gatgagatgt
    24721 tagatttaaa tgtaatttct aaagagatag gctattcaaa ataccattta catagaatgt
    24781 ttacatcagt tgtcggattt agtgtacata attatataca aagacgtaga cttactgaag
    24841 cagcgagaat gctaattttt acagaaaaac caattattga gattgcttta atctcggggt
    24901 atgatacaca acgctctttt tcaagagggt ttaaaaaaat gtttaaacgt agtcctaatt
    24961 tttttagaag acatcaagat tttttaccac tacaattaaa atttgatatt cataatagaa
    25021 aaaaattacg tggagatatg gttttagatg ttaaaaaagt taattgtgca gatataaata
    25081 ttgttggcta ttctggtaaa gtaagaaaag gatttcatat tattggaaaa tgctggaggg
    25141 atttgcataa aaataaagag atgatagaaa ataaggtaga tttaggtttt ttaattggag
    25201 ttagtgatta tagcaatttt aaagagattg agaatactcc tacatttaca tacattgcag
    25261 gtgctcaagt agctactttt ggcaaattac ctaatggaat ggaaaaattc gtattaccga
    25321 aaagtaatta tattgttttt acttttattg ggaaaaatga ggatagtatg caaaatgttg
    25381 tagaatatat ctatcaagaa tggttcccta attcaagttg ccagtttaat gaaaataatt
    25441 tatatgactt tgtaaaatat ggagaaaaaa ctgatgagaa tggtttgagt gaaattcagt
    25501 tttgggttcc aatcatttaa aagaaggtat tatgtataaa gtagatggat ttatataggc
    25561 aaagttttac agcagtatgt atttataaat aatttatagt ttgtaggaaa gaaataattt
    25621 tactataaga ttatgataaa atacacaata ctatgacaaa atagtgttaa ataatagatt
    25681 taattttgca taggaaaagt tctataatgg tgtgtattca taaataattt ataatctgta
    25741 ggaaagaaat agttttacta taagattatg ataaaataca caatactatg ataaaatagt
    25801 actaaataat agatttaatt ttgcaaagga taaggtgttt taataaaaat gaatgtagaa
    25861 aacgaattac ttaaaaacct agatagattg catacaacag agttaggcgt tgtaagaatt
    25921 aagaaaaatc tttctttgga gacaaatgat gtagtgaact ggtgtaaaac taaaatagaa
    25981 tctccaaatg ccattattaa tagaaaggga aaaaactggt atataagtgt atatgattgt
    26041 attataacag taaacgctca cagttataca attattacag ctcataaaga aaagaaatga
    26101 attgcaaaat acaaaggtca aaagcatttt ccactgtttg tagtacctat gaagaagtat
    26161 ataaaataat attacttttg aataataatt taaattttgg aggttgaaaa aatgaatgat
    26221 actgtaaaag aaaaattggc agagtcacta acttctgata ctactgatat tttaccttat
    26281 ttgccttatt tattacaaga tttttgggaa ttaggaagtt caccagaaca aatgttacaa
    26341 ttatttagaa agcatatacc ttttaatgag gatacaaaag ttttggattt agcttgtgga
    26401 aaaggtgctg tatctgtgaa gcttgcaaaa gagttgaata tacatatcaa aggtattgat
    26461 ttgatacctg aatttattga ttatgctaga agaaaggcta ttgaatataa ggttgatgct
    26521 ttgtgtgatt ttgaaatgaa tgatgctaat agttctgtaa aaattgagca tgattatgat
    26581 tgtgtgattt ttggagctgt aggggatatt ttaggtaatt ataaagaaac tataagtaag
    26641 ctattaccta caataaaaag taaggggttc attttaattg atgatgcata tgtagtagat
    26701 aaatctacaa atgaacagct taattttgag agggtgtatc ccacttacaa tgagtggtgt
    26761 atattgttca aagaattagg tctgaagcta attgaatgta taccatgttt agaacttgaa
    26821 aatgatgttg aagaggaaat gaattggatt ataactcgtg caaatgagct aattaaaaaa
    26881 tatccttctc aaaaagatat gtttgaacaa tatgttatta gccaaaaaaa tgaatatact
    26941 gatttacagc aggaccttgt tggagcaact tggattttac aaaaagaata agataaatat
    27001 tagactagat aatcaatgtt atagtgaatt atcccatgtc tagtgaatat aaataacata
    27061 aaaaaccgtc aagatgaata gtttattcgt gcttgacggt ttttagtatt tgttgaagta
    27121 aaattttatt tctttagtat atcactttta gtcaatccat ttgatttagc ttgagtattc
    27181 cattgatgat tccccataag tttatatagg aatctaggga agttaggtga aataaaaata
    27241 gtacttttgt tggaatcatc tatatgtata ttatcactaa gtatattttt tgaaagttct
    27301 tttaatgcaa ttcctaaatc ttttttaggt cctttaccca aaggtacgga ttctagtgaa
    27361 ccaaacatct ccccagctcc aattccaata ccttgaaccc atgtaaagtt gattttttta
    27421 caccaatgct ctatcatttg aattgccaaa tgattatgtt caccttcatt gaatccacag
    27481 ttaactattg catacactgg aataggtttt ttattttgtg tttttaaaaa tttttcacat
    27541 ttctctaaaa aatgaagtaa atgagatgga ataccatcta cataaagagg agatgcgatt
    27601 attaatctat cacaattaag tatggcatta agagcgtcag gatacttaga tgtgttatct
    27661 actttaaatg taaaaacttt attatcattt tgaataagtt gttttaattc agataaaaaa
    27721 tactgtgaat tatttttatt tacttttgga ctaccattta tcattactat attcatatgg
    27781 attcacctct tatatcttgt atattttcat agaaacaaac agagtgttct tttatattga
    27841 aatttatagc atttccattt actaatttcg tagcagtttc tttttcagct tctgtaatac
    27901 aatctccata gaatatagct tttaaattta tctgtttatc atatctgctt ttatgacgca
    27961 tcatattatc ttttattaca aagtatggaa gaatataatt aatgctttta tcaagtacgt
    28021 ttttgacaaa agggctaaac cctccgtaac agcatctgct tattataata attccatcag
    28081 agtcatttat aagtttaccc atgttgctgt aataatcttt tacaacacat tcagaaggag
    28141 ttttaatcca gcaaccaaaa caaccaatac atttgtggat ttcttttgtg tttggaagta
    28201 tttttatatt gtcatcaagt ttgggtaata acttttctaa ttctttgtca gttaaatcat
    28261 gtattaaaag cctcataaag tccatctcct tttattatgt atgtttactt ctaaaaatta
    28321 tatcttatag atttttcagt aaatctttac accaattaat aacaaaatta taatagtcaa
    28381 ctccataaat cattgttgca tacatatatg tatattggtc aacacaatga ttttcttctg
    28441 catcattttt ttgtttactt aatttattta gaagtaattc tacttcagtt attaacattt
    28501 ttaagtttgt ttcaattatt tctttgggta gatacttgta aaagaatata ttaacaaggt
    28561 ggtcttgctt tgttttagaa aaatttatag gtttctttaa ccattcaaga aatacttctt
    28621 taccagtatt agttatggaa tatagttttt ttaatttgtt accatctata acttcgtgat
    28681 agtgtatata tccttttgtt tctaatcttt taagtgcagg atagatactt ccaaagctgg
    28741 cgtcaaaaaa gtacgaggta ctttctgaca ttatttgttt taagtcgtat ccacttaatt
    28801 ctttttccat taaaaatcct aaaataatgt attctaacat attacctcct ggtatcaatt
    28861 agatatatat cgattcgata tatttattat tacagataat aaatcttacg tcaagatgct
    28921 ttttaagata agctaaaaag aattaggagt tattgataaa tatgcaaaaa aattaatata
    28981 aaaggtaatt aaatatcaat aaagatttaa aaatagttaa atcaaagtaa gagtctaata
    29041 tataaaattt atagtaactt tatggtttaa atataaactt gtgtgctatt gacaaagtat
    29101 aaaattagca gtagaataat tgcatgagca aatagattgt tgcgaacgca aaaaaaggac
    29161 aatagatatt aaaggagaga atatgagaga gctattaaaa tgggtttcca ttataaatag
    29221 atacaataca aattatattg acaggaaatt ggcggatatt ggaatcaata atagccaata
    29281 tttttatgta ttacacatat gtaataatcc aggtattaca caggacacta tatttgagaa
    29341 aattcttgtg aatcctagta atataacaag agctttggca aatttagaaa aagaaggctt
    29401 tataacaaga gaaccaggtg taaaggataa aagaacatgg catttatatc ctacagaaaa
    29461 atctttagga tgttatgatg aaatactaaa aataacaaat gggtatgttg aggagttgtt
    29521 gagttcattt gagaaagaag agcaagagtt gtttatgtca atgttaaaga aagttgcatt
    29581 tttggctatt gatatgaatg aagaagcaaa attggaggcg aaggaaaatg gaagaaaaat
    29641 ataaggagcc attggaatat gaaaaggttt caaagttatt aactaagtac gcaattccta
    29701 gtattatagc aatgttagta agtgctttat ataacattgt tgaccaaata tttattgggc
    29761 aaggtgttgg tgtattgggc aatgctgcta caaatgtcac ttttccacta acaacaatat
    29821 gtactgcaat tgcattattg ttaggaattg gaggagcgtc taagtgttca ttggaattag
    29881 gtgctaaaaa ttcagaaaaa gctacaaaag cagcaggaaa ttctatatgc ttgatggctg
    29941 tttttggaat aagtttattt atattagtat caatattttt aacaccacta ttaaaattct
    30001 ttggctctac atcagaaata ctaccttatg cacacacata tacaaaaatt acatcaattg
    30061 gattaccatt tcttataatg tccactggaa tgagtaagtt gatacttgca gatgggagac
    30121 cgaaagcttc tatgttttgt atgttaatag gagcagttat taatacaatt ttgaatccat
    30181 tattcatttt tgtttttaat atgggaattg ctgggtctgc tttagcaact gttattgggc
    30241 aacttatatc ttttggaata agtgttcatt atataaagaa ttttcaacat attaaattac
    30301 ataagaaaag ttttaaatta gatagtataa tttataaaca tatatttgcg ctaggttcaa
    30361 gctcatgttt taatcaatta gcaatgactg taacacagat agttatgaat aatacattgg
    30421 catattatgg ggctaggtca gtttatggaa gtgagattcc acttgcttgt gtcggtatca
    30481 ttattaaagt aaatatgatt tttatatcta taattattgg tatatcacaa ggtatgcaac
    30541 caattatagg gtataattat ggggctgaaa agtataatcg tgtaaaagaa gcatataagt
    30601 ttgcagtggg aagtgcaact attatatctg ttattgcatt cttatgtttc caattattcc
    30661 caagacaaat tacgtctata tttggtaacg gaaatgagac ttattttata tttgcagaac
    30721 attattttag aactttcttg ttcttaacat ttataaatgg tgttcaaata gtatcatcaa
    30781 ctttctttac atcaattggg aaatcagtgt tgggactttt tacttctttg actcgtcaaa
    30841 ttttattttt aattccattg atagtaattt taccaatgtt tatgggaatt gatggagtaa
    30901 tgtttgcagg accaatagca gatgcagcag cggcaatggt atgtatatac tttgcagttc
    30961 atgaaatgaa atcattgact ataaagcaaa ataatttgat gagtaaccat aataagattg
    31021 cagaggtata agacaatatt taagaagtat agaaaattaa tttaaagaag tataataaac
    31081 taatttagga agtatagaaa attaatctaa agaagtataa taaactaatt tagaaagtat
    31141 agaaaattaa tctaaagaag tgtaataaac taatttagga agtatagaaa attaatctaa
    31201 agaagtgtaa taaactaatt taggaagtat agaaaattaa tttaaagaag tataataaac
    31261 taatttagga agt
//
LOCUS       DACULQ010000024        30740 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.024, whole genome shotgun sequence.
ACCESSION   DACULQ010000024 DACULQ010000000
VERSION     DACULQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.024"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            143..964
                     /locus_tag="JA154_04665"
     CDS             143..964
                     /locus_tag="JA154_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAT7995152.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            985..2181
                     /locus_tag="JA154_04670"
     CDS             985..2181
                     /locus_tag="JA154_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA containing CoxE family protein"
                     /protein_id="HAT7995153.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            2348..5746
                     /locus_tag="JA154_04675"
     CDS             2348..5746
                     /locus_tag="JA154_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HAT7995154.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5756..7429
                     /locus_tag="JA154_04680"
     CDS             5756..7429
                     /locus_tag="JA154_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HAT7995155.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7702..7896
                     /locus_tag="JA154_04685"
     CDS             7702..7896
                     /locus_tag="JA154_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HAT7995156.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    8065..8298
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8375..9772
                     /locus_tag="JA154_04690"
     CDS             8375..9772
                     /locus_tag="JA154_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAT7995157.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(9945..11120)
                     /locus_tag="JA154_04695"
     CDS             complement(9945..11120)
                     /locus_tag="JA154_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT7995158.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            11539..12903
                     /locus_tag="JA154_04700"
     CDS             11539..12903
                     /locus_tag="JA154_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995159.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            12925..13416
                     /locus_tag="JA154_04705"
     CDS             12925..13416
                     /locus_tag="JA154_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAT7995160.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13688..14359
                     /locus_tag="JA154_04710"
     CDS             13688..14359
                     /locus_tag="JA154_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAT7995161.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMKRLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14429..15310
                     /locus_tag="JA154_04715"
     CDS             14429..15310
                     /locus_tag="JA154_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAT7995162.1"
                     /translation="MRTTDMKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGL
                     YVGGSTGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGY
                     DAISAVTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENE
                     KIIGVKFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRA
                     RQIFELTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKE
                     ATDEMKSRAKEIYRKYF"
     gene            15343..16845
                     /locus_tag="JA154_04720"
     CDS             15343..16845
                     /locus_tag="JA154_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HAT7995163.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            16975..17427
                     /locus_tag="JA154_04725"
     CDS             16975..17427
                     /locus_tag="JA154_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAT7995164.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            17587..18390
                     /locus_tag="JA154_04730"
     CDS             17587..18390
                     /locus_tag="JA154_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995165.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18448..19335
                     /locus_tag="JA154_04735"
     CDS             18448..19335
                     /locus_tag="JA154_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAT7995166.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKDMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19354..20202
                     /locus_tag="JA154_04740"
     CDS             19354..20202
                     /locus_tag="JA154_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT7995167.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            20265..20954
                     /locus_tag="JA154_04745"
     CDS             20265..20954
                     /locus_tag="JA154_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAT7995168.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(21065..21244)
                     /locus_tag="JA154_04750"
     CDS             complement(21065..21244)
                     /locus_tag="JA154_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995169.1"
                     /translation="MHFHAGKCQLMQGIFQKENTDKALKYGIYRHLPTYEKIIKNLVS
                     FLNKKESYLFFYTIQ"
     gene            complement(21284..22474)
                     /locus_tag="JA154_04755"
     CDS             complement(21284..22474)
                     /locus_tag="JA154_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT7995170.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYAWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLDDGIDTAGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSERGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEPKVILTPEQEERLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLRDSEIGYYIDTPKTKNGKRKIPLTE
                     RAYQALQRILKNRVKAQPLVVGGYSNFLFLNREGLPKVAGNYVGMVRGLIKKYNKYHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(22552..22755)
                     /locus_tag="JA154_04760"
     CDS             complement(22552..22755)
                     /locus_tag="JA154_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAT7995171.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            22872..23109
                     /locus_tag="JA154_04765"
                     /pseudo
     CDS             22872..23109
                     /locus_tag="JA154_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23137..23385)
                     /locus_tag="JA154_04770"
     CDS             complement(23137..23385)
                     /locus_tag="JA154_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT7995172.1"
                     /translation="MKHTFKKPSYYLLSQAMDGDEKAIEKVLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            complement(23389..23808)
                     /locus_tag="JA154_04775"
     CDS             complement(23389..23808)
                     /locus_tag="JA154_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAT7995173.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQQGLKRRKEKEISFC
                     ELPEIMVENLAVWDDYETDYTIFNVCGNDIRVYDDELAEALKQLSERNRETLLMYYFL
                     EMNNEEIAKKQNISRSGVFQNRHNSLALMRKLLKEKR"
     gene            24229..24588
                     /locus_tag="JA154_04780"
     CDS             24229..24588
                     /locus_tag="JA154_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7995174.1"
                     /translation="MRKTKETHTFDFRPLGLAIREAREKAGLSRNDLGDKVFYGERHI
                     ADIENIGSHPSFQLFHDLVTMFNISVDEYFYPAEKVAKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRENKK"
     gene            complement(24707..24895)
                     /locus_tag="JA154_04785"
     CDS             complement(24707..24895)
                     /locus_tag="JA154_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995175.1"
                     /translation="MKNEMWVLCPICNNKTRTKVREDTELINFPLFCPKCKTETLINI
                     KHGKVTIIKEPDAKTQSR"
     gene            complement(24917..25141)
                     /locus_tag="JA154_04790"
     CDS             complement(24917..25141)
                     /locus_tag="JA154_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995176.1"
                     /translation="MIGIKKRDIKKALKAGAKAGGSSYADVLADIEATIDEVMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTRV"
     gene            complement(25165..25355)
                     /locus_tag="JA154_04795"
                     /pseudo
     CDS             complement(25165..25355)
                     /locus_tag="JA154_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            complement(25530..25625)
                     /locus_tag="JA154_04800"
                     /pseudo
     CDS             complement(25530..25625)
                     /locus_tag="JA154_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix family transcriptional regulator"
     gene            complement(25784..26299)
                     /locus_tag="JA154_04805"
     CDS             complement(25784..26299)
                     /locus_tag="JA154_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAT7995177.1"
                     /translation="MKSKPVKVGLRTIKTVLSAFLCFLIDSLRASSVPFYAVIASILC
                     IQPDMENSLSVAKNRELSTIIGGILGMLFLLMERIFLPIQPEILRELTLSILLIPIIN
                     LSVLLKQKKGTFLTCVVFLCVTVTHEKDISPIWFAWNRIVDTSLGIGISLAVNSFPFH
                     KFKVGGTMGKY"
     gene            complement(26289..26789)
                     /locus_tag="JA154_04810"
     CDS             complement(26289..26789)
                     /locus_tag="JA154_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HAT7995178.1"
                     /translation="MYISSIGISNGIIEKKYGSHGVQFNENDVPTYSIPFKIENAPDK
                     TVSFAFILEDKDAYPVTGFIWVHWLGANLTRSELKENESQTAKDFIQGANSWTSILGN
                     QQTREQTSCYGGMAPPDKAHIYELHVFALDKLLDLKNGFYLNELYQEMEGHILEEAVL
                     KGLYEK"
     gene            complement(26877..27242)
                     /locus_tag="JA154_04815"
     CDS             complement(26877..27242)
                     /locus_tag="JA154_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7995179.1"
                     /translation="MKLRDEYTCPLELIHDMIKGKWKPIILWRLRLGATSLSKLENDI
                     DGITQKMLLEHLKELIDYGFVDKKTFSGYPLHVEYFLTEDMGNQILEALRIMQHIGIE
                     YLRKNGMENELTEKHVIPD"
     gene            complement(27226..27777)
                     /locus_tag="JA154_04820"
     CDS             complement(27226..27777)
                     /locus_tag="JA154_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAT7995180.1"
                     /translation="MKKALLIIDVQNDYFPNGKCQLYKPEIALNTIKDLLKIFRKQNL
                     PIIYIRHESIHGTFFNPNTNGVQIHNDIKPLDTETVIVKHYPNSFYETNLQNKLVEIE
                     VTKLVVCGMMTHMCIDTTIRAAKDYGYEITLISNGCATKELKWNGVNIPADTVQSIFM
                     ASLNQKFANVITSTEFLSNEITR"
     gene            complement(27867..28226)
                     /locus_tag="JA154_04825"
     CDS             complement(27867..28226)
                     /locus_tag="JA154_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7995181.1"
                     /translation="MKLRESYTCPLELVHDIIKGKWKTIIIFQLRNGKCSFSELHHSI
                     AGITQKMLLEQLRELKHFGLIDKKTYDGYPLKVEYFLTGQGEKMLSAIKIMQEIGIDY
                     MVEHGMTEILDEKGICY"
     gene            28364..28723
                     /locus_tag="JA154_04830"
     CDS             28364..28723
                     /locus_tag="JA154_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7995182.1"
                     /translation="MRKTKETHTFDFRPLGLAIREAREKAGLSRNDLGDKVFYGECHI
                     ADIENIGSHPSFQLFHDLVTMFNISVDEYFYPAEKVAKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRENKK"
     gene            complement(28842..29027)
                     /locus_tag="JA154_04835"
     CDS             complement(28842..29027)
                     /locus_tag="JA154_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_687936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995183.1"
                     /translation="MKDKWILCPVCGNKTRLKIRADTELKNFPLFCPKCKTETLINIK
                     HGKVTIIKEPDAKTQSR"
     gene            complement(29066..29353)
                     /locus_tag="JA154_04840"
     CDS             complement(29066..29353)
                     /locus_tag="JA154_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995184.1"
                     /translation="MSTLIIGFIVAGVLLIAEYLLCTKFRSPLWGGIIPLLILAGTIY
                     VFASGRIPFERPYIFPFVVVNTLFFGDWGTGRDKYKKIKQTEMEKMKAKDI"
     gene            complement(29356..29652)
                     /locus_tag="JA154_04845"
     CDS             complement(29356..29652)
                     /locus_tag="JA154_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995185.1"
                     /translation="MELDKIPQWILALEQEDATFLKNFALKSGSLKEIAKMYGVSYPT
                     VRLRLDKLIQKIEMCDQQEEEPFQTFIKGLAVDSRIDVETAKIIIEKYKKEKEK"
     gene            complement(29741..30646)
                     /locus_tag="JA154_04850"
     CDS             complement(29741..30646)
                     /locus_tag="JA154_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995186.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKSVIALWVVLIASVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQALNTDTIRKDIPVSSAVNGVQIWSVTQADENQFSVIFTVEQLITENGSTKT
                     VRSAYEVTVYVDGSGNMVLTKNPTITSIPVKSGYTPKAKESDGTVDAVTTDEINEFLT
                     TFFKLYPTATAKELSYYVNEGVLKPIGKDYVFSELVNPIYNRIDSQVTVSVAVKYLDQ
                     QTKATQVSQFDLVLEKSGSNWKIIK"
BASE COUNT        11034 a         4200 c         5329 g        10177 t
ORIGIN      
        1 taaatatttt agaggtgatt ttatttagat atagaatttt agaataatac tatacaaatg
       61 tattgtatta ttatttttat atcaaaaata gtaataaagt ctatatacat ttagaaaata
      121 ctgtttaaaa ggagtaggat ttatgataaa aaaagagata tctaacttta gaggaagtag
      181 tgattatgta gtatcaccag aattgatggc atcagttaat gttgctattg ctcttgaaaa
      241 gccacttttg attaagggcg aacctggaac aggtaaaact atgctagcac aagcaatttc
      301 aaatgaatta aaaaaagatt tagtaatttg gaatataaaa tcaactacaa aagcacaaga
      361 agggctttat gtatatgata ctgttcaaag actttatgat agccaatttg gaggagaagg
      421 tgtagatgat attagcaagt acataaaata tggtaaacta ggagaggcct ttagctcaaa
      481 tcaacaagta attcttttaa tagatgaaat agataaggcg gatttagaat ttccaaatga
      541 cttgctttgg gaattggata aaatggagtt ttacataaat gagacaaaag aaactgtaag
      601 agctaaacaa agaccaatag ttattataac ttcaaatgcg gaaaaagaac ttccagatgc
      661 ttttttaaga agatgcatat ttcattatat agaatttcct gatagagaca tgatggaaga
      721 aatagttaaa gtacattttg ataaagtaga agaacaccta ttggaacaag taatgactac
      781 tttttattgg attagaagtt taaaggacat acaaaaaaag cctagtacat cagagttaat
      841 agactggata caagcattaa ctttgagtgg aatgcctatt gaaaaaatag aaaaagaagt
      901 tccttttgca ggaatactac ttaagaataa tgaggatatt gaatctatgc aaagacactt
      961 atagttaata ggagctggat aattatgttt attgaatttt tttatttatt gaggtcaaga
     1021 ggattaaatg tatctttaaa tgaatggatg actttaattg aagcattgga taaaggactg
     1081 tgctattcaa acttttcaaa tttttactat ctttgcagga tgattttaat aaaaagtgaa
     1141 agtgattttg ataagtttga tgcagtgttt ttggagtatt ttaaaggtat agaacaccaa
     1201 gaagaaattc cagaagagat tatgaattgg cttaaaaaac cagatataga tttagaagag
     1261 tttgaaagat tagaacaaaa tccaaatata aatcttgata aattaagagc taaattggag
     1321 gaacgaataa aagaacaaga ttctcctcat aatggtggta attattggat aggcacagga
     1381 ggcacttcag agttaggaca ttcaggaaaa gggcaaactg gtatacgtat aggtggaaat
     1441 tctacttatg gtagagctgt tcttgaagtt gcaggagaaa gaaaatatag agattttaga
     1501 gatgatgaag ttttaaatat gcgtcaattt caagttgcac taaagagact tcgtcaattt
     1561 tctactagaa tagatgctcc aaagacagag cttgatttgg ataagactat tgaagagact
     1621 tgtaataatg caggatattt aaagttattt tttgagaagc caagaaaaaa tacagtgaag
     1681 cttttattgc ttatggattc aggtggttca atgagagggt atagcacttt atgtaataca
     1741 ttatttcagt ctgtaagtaa atcaaatcat tttaaagatg taaagatata ttatttccat
     1801 aactgtattt atgatagatt atttacaaca cctgaatgtt ggttaagtaa atctattaat
     1861 actgaatgga tattaaaaaa tatagataaa aactataaag ttataattgt tggagatgct
     1921 tctatgtcgc cttctgagtt gcttcatatt ggtggtaact atcgtggtcc atataattac
     1981 acacctggta tagagtggct taaaaggttt aagagaaagt acagtaaaat tgtatggatg
     2041 aatccagaac ttagagatgg atgggattca attaattatt ggtatcaaac tcaaagaatg
     2101 atacaaagtg aatttaatat gtttccattg actgtaaaag gtcttgagaa agcattgaaa
     2161 aatttaatgg tttcaagata aagtaaatat aaatgatggt atgatttatt caaaattgta
     2221 ccatcattat ttattttttt aatataccta ttataaattg tataactgaa acttaaaaaa
     2281 ttatatagtc taatacattt tagactcaca caaaacatat acattaataa taattttaga
     2341 agtcgaggtg attataataa attatgaatt aattgtaaaa tacaatggag atatattgag
     2401 gttagaagaa gaattaggtg tatcagtaga gatacttaat tcatcatatg caataataac
     2461 atcaagtaat gaagaagatg taaatatact tttaacttat ccagaaattg aatttataga
     2521 aaagcctttt atattacaga ctcaggatgt gcaaagtttt tcaagtacag gaataacagg
     2581 atttaaaaat agaactggtt taactggaaa aggaactata attggtataa ttgattcggg
     2641 aatagattat actctacctg tgtttagaga tagtgatgga aggtctaaga tattatatta
     2701 ttgggaccaa tctattcaag gaaatccacc agaaggtttt agagaaggaa cattatatac
     2761 taatgaagat ataaataatg caatagatgg aagtatgtat atacctattt caacaactag
     2821 cttacatggg acacatgttg caggtatttg tgcaactatt gcaagtgatg ccagaataat
     2881 agtggttaga gttggaaata tacaaaccga tattttttcg agaagtacag agtttatgag
     2941 ggcaattaaa tttattttag atagggcatt agaattaaga atgcctgtta cattaaatat
     3001 aagttatgga agtaatgaag gttcacatag aggaacatct ttatttgaac aatatataga
     3061 tgatatgtgt ttattttgga aaaacaatat agttgtagca gctggaaata atgcagataa
     3121 aggaggtcat aaaaggatta ggcttcaaaa taatattaca gaagaagttg agtttatagt
     3181 tggggaagga gagcgtatat taaacataaa tatatggcca gattttgttg atgattttag
     3241 tgtacatttg gtaaacccat caaataatca aacacaagca atctctctaa cttcaggtga
     3301 gataaggaat actttgggtg aaactagaat aactggttat ttttacccta tagcaccgta
     3361 ttctctcaca agaagagtta ctctacaatt aagttcaaat acacagataa ctcctgggtt
     3421 atggaaaatt gtatttgaac caatagatat agtaactggt aatgtaaata tatatttgcc
     3481 aacttctgaa ggtctaaata gaaatactag atttctgatt cctactcaag agcttactgt
     3541 aacagttcct ggaacagcaa gtagagttat aacagtaggt agttttaatt caagaacaga
     3601 tatagtatct atattttctg gtgaaggaga tacacaatta ggtgttttta aacctgattt
     3661 attagctcca ggtgaagata tagtatcttt tttaccagga ggtacaagtg gagcattaac
     3721 tggaactagc atggctacac ctcatgttac aggggtttgt tcattgttta tggaatgggg
     3781 aattgtaaat ggaaatgatt tatttttgta ttcacagaaa ttaagggcat tacttctaaa
     3841 gggagcaagg agattaagta atcaatctta tccaaataat tcatcaggat ttggtttttt
     3901 aaatttatca gatatagatt tgtacactct atctaatata aatcaagatt tagaaacaga
     3961 ggatattgga tatagaagta ttaataagtc atttaaagat gaggaaaata gttataagtt
     4021 tatagatggt tataatatgc aaatacataa tgacttagag aatgaaattt atataagtaa
     4081 aaatgcaagt agacaatcag gaatactatc aggaatagat atagtccata ctcctgaatt
     4141 tgaagaagaa ctagctgggt taggtatgag tcaaaggttt tttaagataa gtgattcatt
     4201 aggtgtacta tctataaata atacagacta taatagcata caacgagttt tgcaacttcc
     4261 ttcaatcata agaactgtat cgactacaaa gatgacatta cttggagaaa ttaatcgagg
     4321 tacttttgga ggtgtagttg caacagaaga aatgggcgta aatttcttta aaaataatcc
     4381 taatattaat ataacaggaa gagggactct aatatcaatt gcagatacag gaatagacta
     4441 tctacaccca gattttattt atccagatgg cacttcaaaa atagtatatt tatgggacca
     4501 aacaaaagaa ggaactcctc ctgatggttt ttatattggg acagaatata cacgagaaga
     4561 tataaatcga gctatcgcag aaaatgaccc tagtctatct caggatgaag ttggtcaagg
     4621 aactatgttg agtgggatat gtgcagggct tggaaatgta aatagtgaat atgcaggtat
     4681 agcagaagat tctgagctaa taataataaa actcggcaag atagatgatt tttataatag
     4741 tgcaatgctg tttgctgcat ctcagtatgc atataaaaaa gcttttgaac ttggaagacc
     4801 attagttata aatatgtctt taggtaccag tagcctagca ggtttaacca ataggagtaa
     4861 tagtgaaaaa gcgttcttta ctagagggtt atgtataacg gcaggagcag gaaatgaggg
     4921 caatacacag acacatacat ctggaataat accatatgtt ggaggttctg ttgaagttga
     4981 attagagtta aatgaagatg aggaagaatt atcattagaa ctttggttaa atagaccaga
     5041 taaagctgat gtaataatag tatctcccac tggtgaagaa agtaaaagtg ttggaatttc
     5101 aaattataat aaagtaacag gtttatttga tttagagggg actgaatatt ctataacata
     5161 tatataccca acaacttttt ctggacagca atttactaat gttactttaa agaatgcaaa
     5221 aagaggtgta tggaagattc gattagtagg agtttatatt ataacaggaa gatataactt
     5281 ataccttcca aatagagaac ttttaaaaag tggaactaga tttagagagg tagacccttt
     5341 ttacactata aattatcctg cgattcaaga tgatttaata accataggag cctacaatac
     5401 tattaatggt agtttatggc aaagttcatc aagagggcca actattgaag acaggttaaa
     5461 accagatata gtagcaccag gtgttaatat aatagctgct tatcctggga acacgtatgc
     5521 aactataact ggtacagcag cagcaagtgc acatgcagct ggtgctgctg caatgtattt
     5581 tcaatatact tttgttgacg gtagatatcc aaaccaagca tatgtgcaaa aaataaaaac
     5641 ttttatgcaa gctggtgcta gaaaagattc aaacacagta tatccaaata caaattctgg
     5701 atatgggctc ttggatgtta gaggaatgtt tgatgtttta agataggagg gatttatgga
     5761 aaaatcttat tgtataattt atcaaggtga tatagaatct gccctacaag aaaatggaat
     5821 aaataggtat atggttttaa atagtcagct tgcagtaata tatgtacctt tagattttga
     5881 tgaaactatt ttaaataata taatacaagt tgcttggtgg gaagaatctg aaccaatgag
     5941 cagtctaatt gaaataacta ataatgtaaa taatggagaa accattacaa cagcagctga
     6001 aactgattat atttatgaaa atccatataa tgatataaca gggagaggaa ttttattagc
     6061 agttatagat tctgggatag attatttgca tcctgatttt ataaatgatg atggaactag
     6121 taaagtttta tatttatggg accaagaagc aaatacaaat ccacctccag aaggttttat
     6181 ttttggaagt gaatttacaa gaagtgagtt aaatatagcc ataaatagaa atgatgggag
     6241 tttgagtcaa gataatatag gtactggaac tttagtttct ggtatattag ttggaaatgg
     6301 aaggataaat tctcagtaca gaggtattac tacagaatca gacttaatag tagttaaatt
     6361 aaaatcatat acagatactt attatgctgg aaggataaat tacagtgttt cagatttttt
     6421 agcagctata acgtatgtta ctaacatagc aagaacagaa aataaaccac tgataataaa
     6481 tttgactatt ggagttaagt caagtgctgt agcaactact tcaatactag atacttttaa
     6541 tatattgagt agtgctggtg tggtagtagt gagtggtgca ggaaatcaag ggaatacaga
     6601 tattcattat tctggcagat ttagcagtgt gggagaagtt caagatgtaa taatacaaga
     6661 tggagatgac tatgctcttg atataacact aaatacgaat ggtccagata aagtgggagc
     6721 acagataata tctccttcag gtgaagttag tcatgatata aggtattctc cagactttta
     6781 tatatataga ggaaaattta acttagaaaa tactacttat gcaatgagat ttatttatcc
     6841 atatattacc tctggaaaag aaaatttgga gataagatta agagatataa aacctggagt
     6901 atggatttta agattgactt cagaacttat aataaatgga gaatatgaca tatacttgcc
     6961 aaataaaaat ttgatagctc cagacacaag gtttttagac cctgattcag tagctactat
     7021 aactatgtat gctgctagtg atgatgtaat tacagttggt acatttaaca ataagactga
     7081 tagtatgtgg attggttctt cgaaaggacc aattagagga agaggaatta agccagatat
     7141 agtagcttca ggtgtagata taatatccac atataaaaat ggaacatata atacaggaac
     7201 aggaacagga gtgagcagtt ctatagtcac aggtgtttta gccttattga tggaatactt
     7261 agaaaaacag gataatgtac cgagattatc attgtttaca caagttttaa aaacatattt
     7321 aatattgggt gctacaaaac ttgagatata tacatatcca aatgtatcgc agggatatgg
     7381 gattttaaat ttaaaaaata cgattcaaca gatagcaaat actctataga taagaaccta
     7441 tgtaaaatta taattgatta ttagtttaat tgtatcaaat actttagata gagtagaaaa
     7501 atagcataaa aaataaatat gaaaattaaa aaacagtttt atattttgaa tacacaaagt
     7561 aaatattttg tatataaata ggtataaatt aagtattgaa aagatatgat gttaatttaa
     7621 aaataattat aatttagact tatgaatttt atttgtaaaa ttgctaaaac tattacaaaa
     7681 taaataatag aggtgatagt tttggaaagt aaaaaggcaa gagcattaaa aggtaaagat
     7741 aataaaagac ttaaaagttc acttttagtt ggaaatgagt cgacagcagc atgggctgat
     7801 gttgaaaaat taaaacctca tagtaaagtt tcaatacctt caattaaggg tgtcgaagaa
     7861 gcaaaagact gggttgacaa tggaagtaaa ttataaaatt ttggaaattg aagtaataaa
     7921 gaagtaatga agataagtat ttattatcat tatgtaaggt agaattatta tattttaaat
     7981 aacaatcagt atcaattata taaatttatt gactaaaaat ttataaattg gtataatttt
     8041 gttaattagc agaaataata aaagataaat actgtgaaga gaagagtaga tataaaatgt
     8101 agttctagcg agttgaggat ggtgaaagct caatacgagg tttatattga agaacatctt
     8161 ggagtttcta atcgaaagct tatttagtaa gtagacttag acggaaggca ccgttatagg
     8221 ctttaagcat tgcttaacta agaggtcatt atgacaacta gggtggtacc gcgagagtaa
     8281 tagtctttcg tccctttata tagggatgaa gggctttttt tattgaaatt atatttaaaa
     8341 gaaattaata acaattatac taggaggatt taaaatgcaa aaggaattta taacagtaaa
     8401 agaattgtac agagaaaaag aacagtacat agggaagact gtaaaagtag ctggatggat
     8461 aagaacatct agagtatcta aaaattttgg atttatagag ctaaatgatg gaagcttctt
     8521 taaaaatatg cagatagtat ttgatgagaa agtagaaaat tttaaagagg taggaaaatt
     8581 agcaataagc tcatctatac ttgtagaggg agagttagta tctacagaag gggctaaaca
     8641 accagtcgaa atacatgcaa aaagcatagt tgtagaaggg gaatcagata gttcatatcc
     8701 actacaaaag aagagacata cgcttgaata tttaagaacg atagcacatt taagaccaag
     8761 aagtaatact ttttcagctg tatttagagt aagatcaata gctgcctatg caatacacaa
     8821 attcttccaa gaaagaaact ttgtatatgc acattctcca atcataacag gtagtgactg
     8881 tgaaggtgca ggagagatgt ttagaataac aactatggat ttaaatgata ttccaaaaac
     8941 tgaagatggc aaaatagact atactcaaga tttctttgga aaagaggcta atttaacagt
     9001 tagtggtcaa ttaaatgccg agataatggc actttctttt agaaacgttt atacttttgg
     9061 acctacattt agagctgaaa actcttatac aggaagacat gcttcagaat tttggatgat
     9121 agagccagaa atagtttttg cagaccttga agataacatg gaacttgcag aagatatgat
     9181 aaaatatgtt ataagctatg tattagaaaa tgctcctgaa gaaatggaat tttttaatag
     9241 ttttatagat aaaggattgt tagaaaggtt aaataagata gtaaactctg agtttattag
     9301 aataacgtat actaaagcag tggaattatt aattgaatct gggcatgaat ttgagtatcc
     9361 tgttgagtgg ggatgtgacc ttcaaactga gcatgaaaga tatataacgg agcaaatata
     9421 taattgtcca gtgtttgtta cagattatcc aaaagatata aaagctttct atatgagaat
     9481 gaatgaagat ggtaaaactg ttagagcaat ggatttacta gttcctggag ttggagaaat
     9541 cgtaggaggt tctcaaagag aagaaagaga agatgtattg ttagatagaa ttaatgaaat
     9601 gggattaaga gaagaagatt attggtggta tttagaactt agaaagtttg gtactgctac
     9661 tcactctgga tttggtcttg gatttgaaag aataattatg tatatgacag gtatgtctaa
     9721 cataagagac gttatacctt tcccaagaac tcctaaaaat gctgaatttt aataattata
     9781 tataataaaa cttatataac tttaagtata aacactaaaa tgacttgcat atttatctat
     9841 tttagagata tgcaagttat ttttattgta ctataaatat ataatttgat tacttttatt
     9901 ttgcgatata atacttatag gtttatgtaa tagtatgtaa ccttttaata aaatgcaaca
     9961 caattacgtc cctgttcttt tgcagtatat agagccttat cacaattact tataaactca
    10021 tttaatgtta ttgtctttcc aattttcttt gtataaactc caatactaac agttacacat
    10081 tttaaaggaa catcatcacg ctcaaaattg caggactcta taatttttcg taaacattct
    10141 gatttaagtt tagcatctgc ctcattacag ttaaaaatac atataaaaaa ttcctcacca
    10201 ccatatcgta tagcatgttc attttcagaa cttgtaaaat ctaaaattac tttggcaata
    10261 ttttgtagac aatagtctcc cttttgatgt ccataattat cattaaattg tttaaaataa
    10321 tctatatcta tcatcattcc agttataggt tcttgatttg catggctgat taattttttt
    10381 tgtagaatat cttcaaggta tctacggttg ttcattttag taaggtcatc tgtcataact
    10441 ttgtcgttaa gtaaattatt tatatgagat atttcttcat attgttgact aattaaaagt
    10501 ttatcatagt aagtaaatac acgatttcta tatagcattg tagaaacaaa aatggcaagg
    10561 gatgttataa aaaatgtgtt gacaatatta ctaaactcat tgcctggtat agtttgaatc
    10621 ccaggtaaga ataaattaag aaacaaaaag catcctccaa aaactattac tgattctcca
    10681 aattttaata ctgagaaagc tgcagctgct aacataatat aactaaatac acttaggtca
    10741 tttccaccta actggtcaag tagagtaatt acacaacccc ataggcagaa aaaagtcaca
    10801 taaattatcc ataaatttat ataaaacgta cagcaatcct tttttttatt ccaaaggtaa
    10861 gaaaatacaa acataaagac agaagataca caaaatagtg ttacatacat ataaaaatac
    10921 cattggcgtc ttggtgtgac aaaagggcct ccttctctag ctgagattga tatcatcata
    10981 attatttgag atattactat tattatgcaa atgattttac ccgtatgata aagataattg
    11041 cagagttctt tctcaacatt gtctttgtgt tttttgtcta gaggaaatcc aaaaaatttt
    11101 tttatttttt ctaagttcat ataagcctcc attaaaaata aagcataaaa ataagtattc
    11161 ctttctatca ttagatattt ggaatacttc gtttatgatt taattatatc aaaatatatt
    11221 gtgtgtaaca atctaaaatt gtgatagata aaatttatta gataatgtcg agtaacttta
    11281 ttgattaata ctttattata attaagattt acagatagtt tactactgaa aataaagaat
    11341 agaataaatt taaattaagc ataataaaat ataaataata aagttcgcat taacaaacta
    11401 tttaaatatc aagacctgtt aaaaaacaac agcaataaac aaataaaata gataaatata
    11461 tccaattaag gtataattaa gttttatatg atatagttat atttgataat ttacaatagt
    11521 aaaacttgga gggaaaaaat gggtgaaaag aaaataaata atatgaatta ttcacataat
    11581 aattattcca aaaagacagg tgaaccaact agaaaaataa aaaatagcca tatcactctt
    11641 aaaagtaaag aagaaaaaat ttgttctagt tgcaataaag aaaataatac taaggataat
    11701 tactgtaaat tttgtggaaa tgaactttat gaaatagcat cattaagacc acttgaaaca
    11761 aaactagact tgaaatctaa aataaaagag ctttcatatc atgcaaataa aagaggtgta
    11821 tttttaacaa catttacaac catatttata ttgtttatta ttgcattgat atttaaagct
    11881 attataacta ttcaatttaa tgatattagt tatttagtaa atccagcaca tataatccta
    11941 gctctcaact tgggtcaaat tagtgtatct atgtctacca tgatgggttc tgggtttata
    12001 aatgccaata taggtttgtt gatattactt ataatcccta ttttggtatt attaatatca
    12061 aacctaattt ttatgagaaa aagatgtaga gattcaaaaa ctgtattagc aaattctcta
    12121 ggagttggaa ttttttatgg attagtttta gctattttat ctatttttac taatgtaaaa
    12181 actagttcac atagtatgtt agaatatgga tatgcattag aatatagtta tgaatttttt
    12241 agtgttcttt taaatggatt cgtattaggg tttatatgta catacataac tacttataaa
    12301 aaaagttatg aaaaagaaaa tatgtattta tctctttttt caaattctat aagaacattc
    12361 atattaggtt atgttttagt attagtaata ctattggtac taactatatc tgatagtagt
    12421 tacttaaatg aactagatat gagtagttat tcaaatggat taaatttatt tactatactc
    12481 ccacaaatag ctagttatat gtgggctttt gcaaatggaa tttcagtaac cataataaac
    12541 tcaacagtat caatgtttac tttaagttct tcatctttat ttggagatac taaattaatg
    12601 ttttatgcaa tgggagcatt gtctatgtta atattattat taaatggata caagctaaga
    12661 tttaagtata atacagatag tataagacct ataatagtat ttagtatata ttatgcattt
    12721 ttgatgggaa tattggcctt atttagtaca tttatactag atagtaatat taatttcttt
    12781 aatacaacta attatggaac aactttaata atgcaattta aagtcttaca agctattgta
    12841 atttcatttg tatatagttt tgtgatatct ttaataggat ataagctaaa ttcagctgat
    12901 taaatatgat tggagggaat ttctgtggaa ggaaatacta ataataaagt agatgataaa
    12961 ttttctaagg ttaagaaaaa gtttggtagt ggaaaattta aagttgaatt atataaagaa
    13021 aaatcaaaat taaatagaga gattgatgat attcaagaaa agaaagcaaa gatattcttg
    13081 gaaatgggaa tactgacata tcaaaaaatt agagaagatt ctattaatga taatagtttt
    13141 gataaattct gtgatgagtt actagaactt gataagttaa tttatgaaaa aaatatggaa
    13201 attaatgaaa tggaaatggc agaaagtaat gtaacttgtg agtgtggtta tgttggaaat
    13261 gtaaatgata aatattgtgc tgaatgtggt aaaaaatttg aattggaaaa tgaatgtgat
    13321 gattttataa tttgtggtta ttgtgaaagt gagatagata cagaggctga attttgtcca
    13381 tgctgtggaa gaaaaattat aatggattta gaataatatt gggaaaccta tgtatagtat
    13441 acatgggttt ttttattgaa attatttaaa tggacttata acagtagtta tataaaatga
    13501 aaataaacac caaaaaaatg aaataaaatt tcttttaaat gtaaaaaaca agctaatata
    13561 taaaataaat cagtaaaaat atagatgcga gaaaaaatat gaaaaaatat tgcaaagaaa
    13621 aagttttcat gttattatta atacataaat caagaaaata aatttcagaa acgaggtaat
    13681 ttaaaaaatg ctagataagg taaagggaag attaatagta tcatgccaag ctttagaaaa
    13741 tgaacctctt catagtcctt ttataatggg gagaatggct aaggctgcta tggaaggtgg
    13801 agctgttgga attagagctc aaggagttga agatattatt gaaataaaaa aagttactgg
    13861 acttcctgtt ataggtatta taaagagaaa ctatgaagat tcagatatat atataacgcc
    13921 aactaaaaaa gaagttgatg aacttttaac aacagggtgt gaaatgatag cattagatgc
    13981 taccaataga gttagaccaa ataatgaaga cttaaaagaa cttataaagt atataaaaga
    14041 aaacggtgtt ctagtaatgg cagatatttc aaattatgat gaggctatca aagcacaaga
    14101 gtacggtgta gattgcgttt caactacact atctggatat actccatata ctaaaacatt
    14161 agaaggtcca gattttgtat taatgaaaag attagttaaa gatttagaaa tacctgtaat
    14221 agctgaagga aaggtaaata ctcctcaaga tttaaaaaag gtctttgagt tgggtgttca
    14281 ttcatcagta gtaggttcag caataacaag accacaatta ataacagaaa aatttgttaa
    14341 agcaattgaa attaattaaa aattaattta taaattaaaa aattattata tttaactata
    14401 tttgaaaatt aaaattggag gtaaagatat gagaacaaca gatatgaagg gaatttattc
    14461 agcattatta gtttcatttg ataaggaagg aaatataaat gaaaaagggt taagacaaat
    14521 aattagacac aatatagatg tttgtaaagt agatggtctt tatgtaggag gaagtacagg
    14581 ggaaaacttc atgctttcaa ctgatgaaaa gaaaagaata tttgaaatag ctaaagatga
    14641 agtgaaagaa gaaataaaat taatagctca agttggttca gttaatttaa aagaagctgt
    14701 agaacttgct aaatttacaa ctgatttagg atatgatgca ataagtgctg ttacaccttt
    14761 ttactataaa tttgattttg aagaaataaa acactattat aatacaataa taaattctgt
    14821 agataataga ttaataatat actctatacc atttttaact ggtgttgata tgagtttaga
    14881 ccagtttgga gaattatttg aaaatgaaaa aataataggt gttaagttta cagcagcaga
    14941 tttctactta ttagagagaa tgagaaaaac attcccaaat aaattaatat ttgcaggatt
    15001 tgatgaaatg atgttaccag ctacagtttt aggtgtagat ggggcaattg gttctacatt
    15061 caatgtaaac ggtgtcagag ctagacaaat attcgaatta actaagaatg aaaaaatatc
    15121 agaagcactt gaagtacaac atgtgactaa tgatttaata acagatatat taggaaatgg
    15181 cttatatcaa acaattaaat tactacttga agaacaaggt gttgaggctg gatactgtag
    15241 acaacctatg aaagaagcaa ctgatgaaat gaaatcaaga gcaaaagaaa tatacagaaa
    15301 gtatttttaa taaaatactt ttagtatggg aggattagtc aaatggtagg atttacttgg
    15361 atagattttg caatactagt ggtttactta ttggcagttt tatttgcagg attgttattt
    15421 tcaaaaaagg aaatgaaggg aaaagagttc ttcaaaggtg atggaactat tccgtggtgg
    15481 gttacatcag tatcaatatt tgccacatta ttaagtccaa tatcattttt atcattagca
    15541 ggaaattctt atggtggtac ttggattttg tggtttgcac aattaggtat gtttatagca
    15601 atacctttga caataaaatt tttcttacct ctatatagta ggttagaaat agatacagca
    15661 tatgaatact tagaaatgag atacgaaagt aaaggtctta gagtacttgg agctttgatg
    15721 tttataatat atcaaatagg acgtatgtct attataatgt atcttccatc aatagttctg
    15781 gctggtctta caggaatacc tgtaaatgta ctgataatag ttatgggtgt aatagcaata
    15841 atttactcat atacaggtgg attaaaggct gtcttgtgga ccgattttat acaaggaatg
    15901 gtattaatag taggtgttac agggacattg ttctatttaa tagcaagtat aaatggtggt
    15961 tttggaacag taatggatac tcttacaact ggacacaagt tcttagcaag taatgaagtt
    16021 atgtttgata aaaatatact atctacaagt gcatttataa tatttgttgg agctggtctt
    16081 aatacttttt catcatatgt atcgagtcaa gatatagttc aaaggtttac tacaacaact
    16141 gatataaagc aacttaataa aatgacatat ggaaatggtg ttttatcaat gggacttgca
    16201 acagtatttt atctaattgg aacttgttta tttatatact atactcaaaa cccagattta
    16261 gctcaaacag ttcaacaaga ccaagtattt gcatcatata tagcttatga attaccagtg
    16321 ggtataacag gaatactact tgcagcgata tatgcagctt ctcaatcaac tttatcgaca
    16381 gggcttaatt cagttgctac aagttggact ttagatatac aaactatcat aacaaaagat
    16441 ataagtatgg aaaagcaaac taagatagca caatatatat cattaggtgt aggtatactt
    16501 tctatagctg ttgcaatagt attagcaaat ggagaaataa agtcagcata tgaatggttc
    16561 aatagtttta tggggctagt actaggtgtt ttagcaggaa tatttgtact tggtgcattt
    16621 tgtaaaaaag caactaaaat gggagcatat gttggattca tagtatcagc aattttagtt
    16681 gtttatttaa aataccgtat gccagaagta acttcttggg catattctct aataacaatc
    16741 acgacttctg tagtggtagg tacaatagtt agtgcaatag aagctaaagt taaagcaaaa
    16801 gtatctttac caaaagctga gactactgtt tattatgaaa aataatattt aaatgataaa
    16861 ttgtcttata gagtgattag ataaataatt tagttactct ataagattgt ataatatact
    16921 ttgtatacaa aagtataaaa atagacttta taattatata gggagatagt gcttatgata
    16981 tttggaaata taaatcatag taagacatat tcaacattgc atgaagattt acttagatgt
    17041 tttgaatatg ctaaagataa ttcattaata gattatgaaa aaggtactta tacaatagat
    17101 ggtgatgata tttttgtgaa tattgtagag tataaaactt gtgaaaaaga agatagattc
    17161 tgggaagctc ataagaagta tatagatgtt catttgatgc ttgatggatg tgaacgaata
    17221 gatataaatt ttatagaaaa tttagaacaa aaaatatatg aagaaaaggg cgatttcttg
    17281 cctcttgatg gtaaaaataa tggatacatg gaacttagaa aaggtgattt tttagtttgt
    17341 tatccggaag atgctcatat gacaggaata aaagttttag aaaaagaaaa tatcaaaaaa
    17401 gctattttta aggttttggt tagataatga taatttatat aaccactagg gctgaaaata
    17461 gtgatttaac tttcagcgat tagtgagact tcgtaaattt tagttagatt actattttca
    17521 gccttagttt tttatgagaa aattaaaaat aattaaaagc ttaaaatttt gtaaaggagt
    17581 taacttatgg aaaatataga tgtattctta ttaataggac aaagcaatgc tagaggattg
    17641 ggaaatccaa aagagagcat cattccaaat gaaaattgtt ttgaatattt gagtactgat
    17701 gaaattataa atatgagatg tgagctagag acttcagaag gtgatggtac tatagcacct
    17761 gcattttcaa atgaatggaa taagcttact ggaaataaag tttgttttat tcacaatgca
    17821 aaagatggtt caagaataaa aaactggaat catgataata actggtttct aaatgacact
    17881 atagaaaaat ttaatacagg ctgtactacc ttaagtaaac attataatat agaaaataaa
    17941 tacgttattt ggattcaagg tgaaagtgat gcaaaatatg gtagtgatgt attgtactat
    18001 aaagaaagtc taaaaaaaat tgcgtatagg cttaaagaag aatgtatgat tgataaaatg
    18061 tttgtaagtt taactggtta ttggcttgga gaagatgaat attttattag aacaagacga
    18121 attgcagcag cacaggagtc ggcttgtagt gaatgtgata tattatgtgt aggaagcaaa
    18181 attgctatgc agtttcatga cagaggatta actattgatg atgttcatta cactcaagaa
    18241 ggtcttaata tgttaggaga ggacttatgt aaaaatattt ataagtatca tataaccaaa
    18301 gaaaaaagtg tattaagtga tactatagac ttatcagaag caaggaaata tatctgtgaa
    18361 ttggaaaaga taagtaaaag atttgtgtaa tataaaattt taaatagagt tgttaattta
    18421 taaaaataat ttgtatggag atatgttatg aaaaattacg tttgtataga tgttggaggt
    18481 acttcaataa aatatggttt tattagagaa gatggattta taattgataa atcaagtctt
    18541 gatactgaag caaaggaaaa aggtggagaa ggaatattag ccaaaataaa agatatagta
    18601 aaaaaatata tagaggaaaa tgaaataagt ggtatatgta tatcaactgc aggtatggtt
    18661 gacccagttg aaggtaaaat actatttgca ttagaagaat taattccaaa ctataaggac
    18721 atgcaattaa aaaaagaagt agaaaaagag tttaatataa aatgtgaagt tgaaaatgat
    18781 gttaattgtg ctggacttgg tgaaatgtgg cttggagcag gtagaggtgc aacatcttct
    18841 atatgtttaa caataggtac tggaattggt ggatgtatta taataaataa taaactgata
    18901 aatgggttta gtaatagtgc tggtgaagta ggatatatga atgtaaatgg aagttctttt
    18961 caagaattgg catctactag cagtctaatt aaaaaagtag caaaaataaa aaatttgaat
    19021 gaaaatgact taaatggaaa aataattttt gatttagcaa agaataatga tgaagattgt
    19081 ttaaaagaac tcgataatat gataaaatca ttagcagttg gaatcgcaaa tatatgttat
    19141 gtaataaatc cagaagtggt aattttaggt ggtgggatta tggcacaaga aaagttccta
    19201 aaaccaaaaa tagacaaagc attaaaggag gttcttatag agagagttta caaaaacact
    19261 aatatagaat ttgcaaagag acaaaatgat gcaggaatgc ttggagcatt atataatttt
    19321 ttaaacaaaa tataaagaat agaagggttt ttaatgagta ttttagaaca attggaatca
    19381 ccaactttta aagtcacaaa atcagataaa ttattaatag cttatataaa agaaaatata
    19441 gatgatgtat tttataaacc aatatcacaa atagctaaag agagcaatat tggtgaagca
    19501 actataacta ggtttgtaaa aaaaatgaat tttaatggat tacaggattt taaagttact
    19561 ttagcacaag aaatatcaac tatcaataaa aaaaatatta taaataaaaa tatacaaaat
    19621 gatgagccag ctttggatac agcaaaaaaa cttttaagtt caaatgtaac cactttagag
    19681 aacacagtag agataatcaa tagtaaggat gtacatgact gcgcaaggct tataataaat
    19741 gcaaagaaag tttacttcat aggtattgga tattctggta taatagcaca ggattcaaac
    19801 tataaattta tgagaatagg tcttaattgt gtaagttttg acagtagtca tacaatgata
    19861 atgatgtctt ccataatgga agaaggagat ttaataattg caatttcaca ttctggtgaa
    19921 acagaggaga ttataaaaac tgtaaagctt gcaagagcca ataatgctaa aattatatca
    19981 attactgaaa ataaaaattc tgaattaaag gatatttcag atgtacactt atcttatgta
    20041 tcgggagaaa cagtactgga aaccggttca atatcttcta aattagcaca atttttcatt
    20101 attgatttag tatatacaca ggtagtaaaa gaactttcta atgaagctat tgaaaggaaa
    20161 attaaaacta caaatgctat aaaattgttt aaaaatgaat aaaataagaa atttgtaatg
    20221 ttttatagag ctctcttaaa aatttaggaa tggagagata ttacatgtct aaattaaaag
    20281 attataatat tgataaagta ataattttta ataatttaag tactaaaacg aagaatcaac
    20341 ttaaagaaaa agttaaacta aaaagattac aaaaaaaaga aattttattt tatgaaaaag
    20401 atagcttaga cagtgtttat atacttttag aaggaaaggt atctattttt aagataagtg
    20461 aaaatggaga gagaaaagta attttcatat taaatagtgg ggaagtcata aatgatatta
    20521 cacttgacgc ttctaaaaac tctactgttg gctgtgaagc atttgaaaat tcaattgtag
    20581 cttattatag tattgaagaa tttttagaaa ttatgcaaaa tgattttcaa cttacaaagg
    20641 atattatttc ttatatggaa agaagaataa gacgtttata tagacagttg aaaaattcta
    20701 tatctataaa agtagataaa aaacttgcag caaaattata taggttaagt agagaatttg
    20761 gagtttgctg tggagaatgg actttactaa atgtaaatat tacagttaca tatttagctg
    20821 atatgcttgg ctgtaaaagg gagactgttt ccagaatgat aaaggtattg caaaaagaag
    20881 aacttattaa aattgacaat aaaggttttt atgttaaaga aactgaatta tcaaggtatt
    20941 ttaaaaataa ttaattatga ttaagtaaga gtattattag aattttaatt aaataaaaat
    21001 attattaaga gaatctattt atagattctt ttttattgtc taatttatta atttttattt
    21061 taccctattg aattgtataa aaaaacaaat aagattcttt tttattcaaa aaacttacta
    21121 aatttttgat tatcttttca taagttggta aatgccgata tatcccgtat ttgagggctt
    21181 tatcggtatt ttctttttgg aagatacctt gcataagctg gcatttacca gcatgaaaat
    21241 gcaaccaaaa gtagtaaatg agtagtaaat atacgttccg atattaacaa gccagtcttt
    21301 ccagttctgc ttttgcagat tggaatgtac cgtgtgcgta gtagccaagc gtcatggtta
    21361 tgttagcgtg tcccatgaga tattgcaggg tgttcgggtt cattcccttg tttgccatat
    21421 tcgtacaata ggtatgtcgg aatgaatgtg gtgtaatgtt tggtaacttg tctttgtggt
    21481 atttgttata cttcttaatc agtcctcgta ccatacccac atagtttcca gcgactttag
    21541 gcaatccctc acggtttaag aataggaaat tgctgtaacc accaacaacc agcggttgag
    21601 cttttactct gttctttagt attctttgga gtgcttgata agccctttcc gtcaatggaa
    21661 tttttcgttt tccgttcttt gtttttggag tgtcaatata gtagccgatt tcgctatctc
    21721 ttaatagctg gtggtctata ttgagtattc tgttctgcat atcaatatgc gttgtcagtc
    21781 cgcaaaattc agaaatacga agtcccgttt ccagaagaag cacaacctca tcataatact
    21841 tgctgtaaat cttatccgtt tccataaagg caagcaggcg ttcttcctgc tctggcgtaa
    21901 gaataacttt cggctcggta tcatcttcca gaacatcact tagcttaaat tcaaacgggt
    21961 tctttctgat acagtcgtct tgtatcgcca tataaaatga cgctttcaag gaacgcttat
    22021 agttgtcaat cgttttgtag gcatatcctc tttcactcat tctgatagcc cactctttag
    22081 cgtccgactg tttgacggta tcaatcgctc tcatacccaa tgggtcattt ttcagagcgt
    22141 tcataagata ctgtcgtcct ttttccgtag ctcgcttgat gttctttctt tggctgttct
    22201 tcttgtcgta aagctggcag actgtcattt taccgcctgc cgtgtcaata ccgtcatcaa
    22261 ggtctttttt tatctgtgct tctttttccc tcaacgatat atcatcacgt tttccagcag
    22321 gcgttttgtc tgtcggtaca agtttccaag cataaataaa ctgtggcttt ccgtatatat
    22381 cggtatattt ataaacgtat cttccgtctt ttcgctggct ctctccatta cgcaaattgc
    22441 gatttttact gtcttttcgt ttcacattag acatagtgta aagctccttt ccgtcatgga
    22501 atgagccgtg atacgctata cttattatac catatccacg gctctatgac attagatttc
    22561 gtccaatgta tctatgattt tttcaaattg ttttcgcttt atctgaatac ggttgccgtt
    22621 cacgataacc cagccagcgt caatattttc ttcggctaat ttacgcaact tcttttctcc
    22681 aatgcggaag tatttggacg cttcttcaat cgtcagtgta tatttttccc agataggcac
    22741 atcagtatta ttcataattt atgcaccccc tttactgtgt gtcagtctat aagttatata
    22801 aacaggcaga cggctttgga aagctccatt agtatttcaa ctattcccat tcttgtgggc
    22861 agaaccgcac catgcggaag tatcattatg ctgtaagggt aggtcatggc aaaacgactt
    22921 ttccaacggt cgctctcgga tcactgcgtg ggtcgctcgc tttcttttgg aaaggtcatg
    22981 gcgtacagtt cccgtggctc tctatcctta caaacgtatc tgtctgcttt attcagtttt
    23041 caaagagctg tggcgaaagc ggtatgcttt catgtaaaag caggagcaga gcaggaaaga
    23101 ggggattgaa caaatcatgc tctgcgttac tgctggttat tcttcttcaa tttctaaggc
    23161 aatatcaaac cctaaaatca ttttgataag tgcttctcgg ataagtcctt ttaactccat
    23221 atctacaacg atataaacat taccgtattc atcatagagt ggacgcaaac agcacttcga
    23281 tatgtaaggg tcataaaatg caagtacctt ttcaatcgct ttttcgtccc cgtccatagc
    23341 ttgtgaaagc aagtagtaag acggcttctt aaatgtgtgt ttcatttgct atcgtttctc
    23401 ctttaatagt tttctcataa gtgccaatga attgtggcgg ttctgaaaaa ctccgcttct
    23461 ggaaatgttt tgctttttgg cgatttcttc attgttcatt tccaaaaagt aatacatcaa
    23521 caaagtttct cggttacgtt cggataactg tttcaaggct tctgccaatt catcatcata
    23581 gacacgaata tcattaccgc atacattgaa aatcgtatag tctgtttcgt agtcgtccca
    23641 tacagccaag ttttcaacca tgatttctgg aagttcacaa aaggaaattt ctttttcttt
    23701 tcttcgcttc aatccctgct gatagtcctt gacaacatga cggacaactt tcttcaaaca
    23761 gctttcaaaa cggcattgaa cagttttctg gaagtcagac ggtttcatca atctcacccc
    23821 ctttccgtta tgacgtgaaa agtgtttatc cctctttccg caccatatag ggaatggcaa
    23881 gtgtgatttg ttaagttgaa accagaaaaa atcgaaaaaa ctttttagta aaacaaaaac
    23941 agcccgtgtg aatgtgaata cacaaacgga ctgctttata aatgctatct gtatgaattt
    24001 ttaactcttg tagagggtag aatgattgta atactgaact aaaatatttt ttcagttggg
    24061 aacagcaaag tattattcca tatacaatca tggcgtagcc ccctttacca gagctatacc
    24121 ttgtgccacc aatgaaaaag gacggctctg gctatatcca aaaccgcccc gttatgtttt
    24181 caagacgcac aaaagtatat catctcggaa cggttatggt gatttaaaat gagaaaaaca
    24241 aaagaaacac atacttttga tttcagaccg ctagggctgg caatcagaga agcccgtgaa
    24301 aaagctgggc tttctcgtaa tgatttaggc gataaggtat tctatgggga acgccatatc
    24361 gcagatattg aaaatatcgg ttcacaccca agttttcaat tattccatga tttagttacc
    24421 atgttcaata tatcggttga tgaatatttc tatccagcag agaaagttgc aaaaagcaca
    24481 gctcgccgtc agatagaaac atcacttgat ttattgagtg ataacgaatt aaaaattatt
    24541 caaggtacga ttgacggtat cttgaacagc agggagaata agaagtaatc agcttcttgt
    24601 tttccccgtt ttctttttag ccatatttct tcaagactta tttgttgatc tgctttgcag
    24661 gaaacaaata agagccgaca atctataagt tacaataacc tacaaattat cggctctgcg
    24721 tcttagcgtc tggctctttg ataatagtta cttttccatg ctttatattg attaaagttt
    24781 ccgttttgca cttcggacag aacaaaggaa aatttatcaa ttccgtatct tctcgcactt
    24841 tagttcgtgt cttattattg caaataggac ataataccca catttcattt ttcacagaac
    24901 cacccttact aattctttaa actcgtgttt tcttcgtgat tttatagata tattcctctg
    24961 gtgtagggcg tttatttcca aaatactttt tgaaatttgc ctgcacttct gggtcggtgc
    25021 tgttcataac ttcatcaatc gttgcttcaa tatcagccaa aacatcagca tacgaagaac
    25081 caccagcctt tgcaccagct ttcaatgctt ttttaatatc tcgtttttta attcctatca
    25141 caagaaccaa ctccctaaaa tacgttaatc ttctttttca aaaattaaat ctatctttcg
    25201 catacagcct tttgcgtcaa tttctgtagg cacgaacccg catagcgata cccattttct
    25261 gcatagctat ttataacttc cttatgttta tcggtgcaaa ggaatacaac acctttcgca
    25321 gtataatcaa tttctaaata ttcatatttt ttcatttttc ctttcttttt catattttat
    25381 ctgtattttt tgattgcctt atttcttaaa ggaataccca cacccaatat caatactact
    25441 accaaaatag ataaatccat aagcacacct cacttttcta aaattctttt ttcttcatta
    25501 ttgccagact aataaaaatt gtcatatccc catacctttt cataaatctg ttcttttgtg
    25561 aacacctgtc ctttgtggct ggaaaggaaa tatagtaggt caaattcttt agcagtcaga
    25621 tatatagacg ttccctcttc gttggtaaag gtacgggtta atgggtctat atcaaatcct
    25681 tttttctgct gattaaaaga agcaggggtg taagcaagtt tagaggacag ccattccacc
    25741 gcttgttgga aaacagcgtc ctctttttca gtaaattcaa caatcagtat tttcccatag
    25801 taccacctac cttaaattta tgaaagggga aagagtttac tgccagtgaa ataccaatcc
    25861 ctaatgaagt gtcaacaatc ctattccatg cgaaccaaat aggacttata tctttctcat
    25921 gtgttactgt tacgcataag aatacaacgc acgttaaaaa tgttcctttt ttctgtttca
    25981 acagtacaga cagattgatg attggaatca ataaaataga taaagtcaat tcccgaagta
    26041 tttctggctg aatgggcaaa aaaattcttt ccataagcaa aaagagcatt cccagtatac
    26101 cgccgataat tgttgaaagt tccctgtttt tagcaacgct caaactgttt tccatatctg
    26161 gctgtatgca cagtatagac gcaatcactg cataaaaagg aacacttgaa gctcttaagc
    26221 tatctatcaa aaaacaaagg aacgcagaaa gtaccgtctt aattgtccgc aatccaactt
    26281 taacaggctt acttttcata caaccctttt agtacagctt cttcaaggat gtgcccctcc
    26341 atttcttgat agagttcatt aagataaaat ccgtttttta aatcaagcag tttatcaagg
    26401 gcaaatacat gaagctcata aatatgtgct ttatctggtg gtgccattcc accataacag
    26461 cttgtctgtt ctctggtttg ctgattcccc aatatgcttg tccagctatt cgcaccttgt
    26521 ataaagtcct ttgctgtctg gctttcgttt tcctttaatt cacttcttgt tagattagct
    26581 cctaaccaat gcacccatat aaaacccgtc actggatatg cgtctttatc ttccagtata
    26641 aaggcaaagg aaaccgtttt atctggtgca ttttcaattt tgaacggaat tgaatacgtt
    26701 ggcacatcat tttcattgaa ttgcactcca tgactaccat attttttttc aataattcca
    26761 ttagaaattc ctatacttga aatatacata aattttcctc cttgtatatc gtttaattac
    26821 agatatatat taacaaagag gaagcaaaac gtaaattacc cacttttttg taggtattag
    26881 tctggaatca cgtgtttctc agttaattca ttttccattc catttttcct taaatattca
    26941 atccctatat gttgcataat tcgtaatgct tctagtattt gatttcccat atcttccgtc
    27001 aaaaaatact cgacatgaag tggataacca gaaaatgttt ttttatcaac aaatccataa
    27061 tctataagtt cctttaaatg ttccaaaagc attttttgtg taattccatc aatatcattc
    27121 tctaatttag ataatgaagt agcacctaat cgtaagcgcc ataaaataat tggcttccat
    27181 tttcccttta tcatatcatg aattaattcc aaaggacatg tatattcatc tcgtaatttc
    27241 atttgacaaa aactccgtgc ttgtaataac attagcaaat ttctgattta aagaagccat
    27301 aaatatgctt tgaactgtat ctgctgggat attgactcca ttccatttta attccttggt
    27361 cgcacaacca tttgatatta gtgtaatctc atagccataa tctttggcag ctcttatagt
    27421 tgtgtcaata cacatatgtg tcatcattcc acaaacaacc aatttcgtta cctcaatctc
    27481 taccagtttg ttttgcaaat tcgtttcata aaaactattt ggataatgtt taacaataac
    27541 tgtttctgta tctaatggtt taatatcatt atggatttgt acaccatttg tatttggatt
    27601 aaaaaatgtt ccatggatag actcatggcg aatgtaaata attggcaagt tttgctttct
    27661 aaaaattttc aataaatctt ttattgtatt caacgcaatc tctggtttat acaactggca
    27721 ctttccatta ggaaaataat cattctgcac atcaataata agtaatgctt ttttcatact
    27781 ctcatatcct ctcttaattc taattaaata atataagata atagtttcta agtaaattac
    27841 ttactttttc gtgtatatga tagagtttaa taacaaattc ctttttcatc aaggatttct
    27901 gtcataccat gctctaccat gtaatctata ccaatttctt gcattatctt aattgctgat
    27961 agcatctttt ctccctgtcc agttaaaaag tattctactt ttagaggata tccatcatat
    28021 gtttttttat caattaagcc aaagtgtttt aattcacgca gttgttccaa tagcattttt
    28081 tgagtaattc ctgctatact gtgatggagt tcggaaaaag agcattttcc atttctaagt
    28141 tgaaaaataa ttatcgtttt ccattttccc tttataatat catgaacaag ttctaatgga
    28201 catgtatagc tttctctaag tttcatttta tcatctccta tgtgaaatat cttacatgtt
    28261 taagctcaat atttcaaaac atattttatt accctaatat atgcactttt aacacattct
    28321 aaatacgaac catgaagtat aaactcatgt atggtgattt aaaatgagaa aaacaaaaga
    28381 aacacatact tttgatttca gaccgctagg gctggcaatc agagaagccc gtgaaaaagc
    28441 tgggctttct cgtaatgatt taggcgataa ggtattctat ggggaatgcc atatcgcaga
    28501 tattgaaaat atcggttcac acccaagttt tcaattattc catgatttag ttaccatgtt
    28561 caatatatcg gttgatgaat atttctatcc agcagagaaa gttgcaaaaa gcacagctcg
    28621 ccgtcagata gaaacatcac ttgatttatt gagtgataac gaattaaaaa ttattcaagg
    28681 tacgattgac ggtatcttga acagcaggga gaataagaag taatcagctt cttgttttcc
    28741 ccgttttctt tttagccata tttcttcaag acttatttgt tgatctgctt tgcaggaaac
    28801 aaataagagc cgacaatcta taagttacaa taacctacaa attatcggct ctgcgtctta
    28861 gcgtctggct ctttgataat agttactttt ccatgcttta tattgattaa agtttccgtt
    28921 ttgcacttcg gacaaaatag tgggaaattt ttaagctctg tgtccgctct aattttcaac
    28981 ctcgttttat taccgcaaac tggacataaa atccatttat ctttcaataa tgcgttcctc
    29041 ctatctaagt atacagtttt gtaatttata tatctttagc tttcatcttt tccatttcag
    29101 tttgctttat ctttttatat ttatccctgc cagtacccca atctccaaaa aataaagtat
    29161 tgactacaac aaacgggaaa atatatggtc tttcaaatgg tattctgcca cttgcaaaaa
    29221 catatattgt tcctgccaag ataagcaagg gaataatacc tccccaaaga ggacttctaa
    29281 attttgtgca gagaagatac tctgcaatta ataaaactcc tgcaacaata aagccaataa
    29341 ttaatgtact catggttatt tctccttttc ttttttatat ttttcaataa ttatctttgc
    29401 agtttccacg tcaattcgag aatcaaccgc taacccttta ataaaagttt ggaaaggttc
    29461 ttcttcctgc tggtcgcaca tttcaatttt ttgaattaat ttgtctaaac gtaatcgaac
    29521 agtagggtaa gataccccat acattttcgc tatttccttt aatgaaccgg atttaagtgc
    29581 gaagttttta agaaaagtag cgtcctcttg ctctaaggca agaatccatt gagggatttt
    29641 atcaagctcc aaaaacacaa ctcctttcat attatcttta acaatattca gtatatcata
    29701 aataaaatta aagtcaataa gaatttaaac atcaaaataa ttattttata atcttccagt
    29761 tgctcccgct tttttccagc accaaatcaa attgtgatac ctgcgttgcc tttgtctgct
    29821 ggtcgaggta cttcacagcc acggaaaccg tcacttgact gtcgatgcgg ttgtagatgg
    29881 gatttaccag ttccgagaat acataatctt tcccgatagg ctttaacacg ccctcattta
    29941 cataatagga cagctcttta gcggttgcgg ttgggtagag cttaaagaac gtcgtcagaa
    30001 attcgttgat ttcgtccgtc gtcacagcgt ccaccgttcc gtcgctttcc tttgcctttg
    30061 gcgtgtagcc ggatttcacg ggtatgctgg taatggtcgg attcttcgtg agaaccatgt
    30121 ttccagagcc atccacatag accgtgactt cataggcgga gcgtaccgtc ttcgtgcttc
    30181 cattctccgt gattaactgt tccacagtga agatcacgga gaactggttt tcatccgcct
    30241 gcgtgacaga ccatatctgt accccattga ccgccgaaga cactggaata tccttgcgga
    30301 tggtatcggt attaagtgcc tgcaattcct ttgtcagata accgctgatt gcctgcgtcc
    30361 ttgcttcaat agcttctttg ctgttattcc atgtgtaata ggacttcgca aagttcttca
    30421 cgaaattttc aatcccgttg gtatcggtaa gtcttaactg tatggtttct gtttcatgta
    30481 cggtgtgcat atcaatagca gtaaaattct tatatacccc aaaactcacg cttgcgataa
    30541 gcactaccca caatgcaatc acagattttt tatgtgtacc gactttcaca gtacgcactt
    30601 tcttttcttt gacattctct gtctgtttct tattcttctt aaacatattt tctaagtcct
    30661 ttcttttatt ttactcgtcc tgcaccgatt aagtgttgtt gccagtaact attatttaag
    30721 tctgcatatc ctatcggatt
//
LOCUS       DACULQ010000025        30670 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.025, whole genome shotgun sequence.
ACCESSION   DACULQ010000025 DACULQ010000000
VERSION     DACULQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.025"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..884)
                     /locus_tag="JA154_04855"
     CDS             complement(<1..884)
                     /locus_tag="JA154_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAT7995187.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQTMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPSS
                     LDTESSIKQGCKYFASLLSSCKNQDIDDLNVAIQSYNYGGGYVGYVAGNGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVFGGSSPTTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVT"
     gene            complement(881..3085)
                     /locus_tag="JA154_04860"
     CDS             complement(881..3085)
                     /locus_tag="JA154_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7995188.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTVSKTGAEKMQEVKRPATTPTTKASEPVKTSGVKERPLSSSTPDGKATQSTYR
                     QNVEKVTVSKETRQNYTKDRRTKVQQTQTVQKNQQTTEKIRNLVTKKGQKKK"
     gene            complement(3085..5535)
                     /locus_tag="JA154_04865"
     CDS             complement(3085..5535)
                     /locus_tag="JA154_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAT7995189.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYEMIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(5513..5911)
                     /locus_tag="JA154_04870"
     CDS             complement(5513..5911)
                     /locus_tag="JA154_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995190.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            complement(6029..6532)
                     /locus_tag="JA154_04875"
     CDS             complement(6029..6532)
                     /locus_tag="JA154_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAT7995191.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            complement(6550..7053)
                     /locus_tag="JA154_04880"
     CDS             complement(6550..7053)
                     /locus_tag="JA154_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAT7995192.1"
                     /translation="MQETRVLVETGRTNGEETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSADMQEEYVELLTVFENLEALYICR
                     NVITVYHDCKSMIDVARQKLMNDPTFKHLSEDCQAYYFDFEAYASHLLENKRYLVTEN
                     SIFELPE"
     gene            complement(6972..7268)
                     /locus_tag="JA154_04885"
     CDS             complement(6972..7268)
                     /locus_tag="JA154_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995193.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     KDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGYWWKREEQMAKKQHHTGLTYR"
     gene            complement(7367..8161)
                     /locus_tag="JA154_04890"
     CDS             complement(7367..8161)
                     /locus_tag="JA154_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995194.1"
                     /translation="MGTINYFLKGIKVLMISDEMRVDILHAVFINKNFIKDEENCNYE
                     KYLLELVNKSIYFREKSNFAEYVPPKSENHGECDCNSPNYQMDFKLLESTTRLHASKE
                     LTGQIQKFCDGVIGKCPPRRPNTQMTVTRLFASLRDYDCESLHSCLTEKYEYGTIEFD
                     IQTYVKLLTFKKNLFFFFPYKFSFNTCYNFKYALDSIRIALEKDFRESNLFREKYYAE
                     YDTFLAYIYEDNLIISKFEQDGKLQMIDCIYLFKSQTYSKLYEYTW"
     gene            complement(8200..8421)
                     /locus_tag="JA154_04895"
     CDS             complement(8200..8421)
                     /locus_tag="JA154_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995195.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(8422..8556)
                     /locus_tag="JA154_04900"
     CDS             complement(8422..8556)
                     /locus_tag="JA154_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995196.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSISKEADYEMKQLEESEDN
                     "
     gene            complement(8569..9765)
                     /locus_tag="JA154_04905"
     CDS             complement(8569..9765)
                     /locus_tag="JA154_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAT7995197.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGIMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKYYQSGELIKHREDDR
                     EYMGSTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFIDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQAILSEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(9950..11344)
                     /locus_tag="JA154_04910"
     CDS             complement(9950..11344)
                     /locus_tag="JA154_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT7995198.1"
                     /translation="MRMILNKGHRIRASDKNLVYRFSMGTLLFVFVAVILLLNTKQLM
                     RTDWEHFSLLDNGFTLSLYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSVFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEQMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNLRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGFLAQARQ
                     DGTATCEAKGDGSD"
     gene            complement(11438..11875)
                     /locus_tag="JA154_04915"
     CDS             complement(11438..11875)
                     /locus_tag="JA154_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_973015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995199.1"
                     /translation="MCEECYSDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPN
                     RGLQRWNFPFKSLEIAKLYLRTADYTTKSSCGIYEIENSKGRVSYKIFAGNEDLHLFL
                     KKNKDKKCKQMTPVFNVGEYKEYPHTEVRKLTSDEIKQYMSER"
     gene            complement(11977..12360)
                     /locus_tag="JA154_04920"
     CDS             complement(11977..12360)
                     /locus_tag="JA154_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995200.1"
                     /translation="MRLSNGFVIDREKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNTGTSQNHTPQGQPKK"
     gene            complement(12376..12702)
                     /locus_tag="JA154_04925"
     CDS             complement(12376..12702)
                     /locus_tag="JA154_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995201.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRKVIARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDVGHTDYVLVADDI
                     VAVEEK"
     gene            complement(12828..15872)
                     /locus_tag="JA154_04930"
     CDS             complement(12828..15872)
                     /locus_tag="JA154_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HAT7995202.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPSTAVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASTRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDKNGNTDVVEVKAGTVYIRELSAPAGYKVDTT
                     VYSLKVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAILPL
                     GTLSVEETKAPNGYLLDGAYMQAGDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETSDTKPQGSATLKDTAFDVISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSAEWTSHK
                     HNTNAGKTSEDGVWFGTSEADDSKGALPYDTYIIEEMRCDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDSEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFIADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKEIEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKAEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDEGQTITFNEKPEVPEKLETPQTPEKPN
                     RPSDSPKTGDSTNVMAFLVMLLTSAGGLAGTYLYKRRTMKKS"
     gene            complement(16115..17479)
                     /locus_tag="JA154_04935"
     CDS             complement(16115..17479)
                     /locus_tag="JA154_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HAT7995203.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHIETCVLLQ
                     RRTM"
     gene            complement(17654..19414)
                     /locus_tag="JA154_04940"
     CDS             complement(17654..19414)
                     /locus_tag="JA154_04940"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089914.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAT7995204.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(19466..20425)
                     /locus_tag="JA154_04945"
     CDS             complement(19466..20425)
                     /locus_tag="JA154_04945"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089915.1"
                     /note="catalyzes the formation of D-fructose
                     1,6-bisphosphate from D-fructose 6-phosphate in
                     glycolysis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HAT7995205.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(20634..24203)
                     /gene="dnaE"
                     /locus_tag="JA154_04950"
     CDS             complement(20634..24203)
                     /gene="dnaE"
                     /locus_tag="JA154_04950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089916.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'-end of a DNA strand by one nucleotide at a time.
                     Proposed to be responsible for the synthesis of the
                     lagging strand. In the low GC gram positive bacteria this
                     enzyme is less processive and more error prone than its
                     counterpart in other bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAT7995206.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(24360..25316)
                     /locus_tag="JA154_04955"
     CDS             complement(24360..25316)
                     /locus_tag="JA154_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation regulator WhiA"
                     /protein_id="HAT7995207.1"
                     /translation="MIDMSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGY
                     KKLNLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEM
                     LLKKLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVT
                     NNEEFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNT
                     KIVKEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIR
                     IEYEDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(25324..25749)
                     /locus_tag="JA154_04960"
     CDS             complement(25324..25749)
                     /locus_tag="JA154_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAT7995208.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(25818..26936)
                     /locus_tag="JA154_04965"
     CDS             complement(25818..26936)
                     /locus_tag="JA154_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995209.1"
                     /translation="MEANMAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVE
                     NREPNVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIR
                     NCLLALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFA
                     ITGRVLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDV
                     IASIYDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYN
                     VLEHVNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGI
                     KTVEKNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(26936..27793)
                     /locus_tag="JA154_04970"
     CDS             complement(26936..27793)
                     /locus_tag="JA154_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adaptor protein RapZ"
                     /protein_id="HAT7995210.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(27824..28537)
                     /locus_tag="JA154_04975"
     CDS             complement(27824..28537)
                     /locus_tag="JA154_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAT7995211.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(28541..29455)
                     /locus_tag="JA154_04980"
     CDS             complement(28541..29455)
                     /locus_tag="JA154_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HAT7995212.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(29728..30498)
                     /locus_tag="JA154_04985"
     CDS             complement(29728..30498)
                     /locus_tag="JA154_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAT7995213.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         8516 a         6659 c         4559 g        10936 t
ORIGIN      
        1 gttacatacg aaccagcgtt ataggtggaa tggaaaaata ccaaatcgcc agcctttgcc
       61 tgcgagagtg gaagatgttg ggtagcgtca tactgggctt gtgccgttct cggtaaggag
      121 ataccagctt ttccatagca ccattgcgtc agtcccgaac agtcaaagga agtggttgga
      181 gaagaaccac caaacacata tttccaccct tgatatttca acgcttcatt cattaccttt
      241 tgtgccagct ctcccgatac ctgcggaaca gttaaatact gattgactaa ttccacatag
      301 aacatatttc catagccata ccgccagcct ccgttcttcg caacggctat cgggttggta
      361 taggttactt tctttccacc cgatttctca cgggcgaagc tctctgcaag attaaaagtg
      421 tgtttctttc cgtttcctgc cacatagccc acatagccac cgccatagtt ataggactgt
      481 atcgctacat tcaaatcgtc gatatcttga tttttgcagg aagaaagcag ggacgcaaaa
      541 tacttacacc cctgcttgat tgagctttcc gtatctaagg aactaggcgg taaaccaaga
      601 ctttctgaac tctgcataac atcttctgcc gtaccgccac tttctacttg aatgatagcc
      661 agtagtacat tgacgtactc ggagataccg ttttctctgg cgtatttttc taccatagtc
      721 tgatgtttca aaacttctgc ggataagttc atacccgtaa tgccagaaga aaagttgctg
      781 ttttcgtcgt cgctgtccgc actaatcagc accccaaaga aaagcaccag agaaaagaga
      841 ataggaaaca gacttccaat gatagcgata tgtttcagtt tcattttttc ttctgtcctt
      901 tcttcgttac aagattacgg attttctctg tggtctgctg gttcttctgg acagtctggg
      961 tctgctgaac ctttgtccta cggtctttgg tgtaattctg gcgtgtttcc tttgacacgg
     1021 taactttttc tacattctgc ctatatgtcg attgggtcgc tttcccgtca ggagtactag
     1081 aagatagcgg acgttctttg acaccgcttg tcttaactgg ttcgcttgct ttcgtagtcg
     1141 gagttgtggc agggcgtttg acttcctgca ttttttccgc acccgtcttt gaaacggttg
     1201 aagctgtggc tggtcgctca tggggacggg tagctcccgt tgtcgctgat ccgtcagcct
     1261 ttcgctgtgc ctgccttgct tcttgtgcct tttgaagctc catacgcttg acagcgatac
     1321 ttttcttatg ctgttcctgc ttttccaagc gtcccgtctg tctggactgc tgttcctgca
     1381 ccataccacg cttgaaatca gacacgctgg actttgcctt ttcctttgcg gaatacaccg
     1441 cataagcggt ctgtgtgggc atatccttga tattctcttt gacagcgttt gccttgtctt
     1501 tcactttatt tttcgtatct aagacagcac ccacctttga gcctgcacgc tgtcccatac
     1561 tggaagtggt attgccccga ttttctttag aagccgtatt ttttctttcg gctctcttgc
     1621 cagcaacggc acttccagct accgcaccag ccacaccgcc cgaaataccg ccagcactta
     1681 cagcccttgc aatacggtgt tccatacgcc tagcccgatg tcgcatgaac agatacggtc
     1741 tgcggaatat cctgcgtccc atgctttgac tgtcgccagc gttgagagaa aacatactca
     1801 ttaagtcgcc cagcttcata tagataccag cgaaacatac tatctgcaag aacgccacca
     1861 tgaaaaacgg atagtccgtg gatatgttat aaaacatact ggaaatgcta aacgcaaccg
     1921 ttacaatgag cgttattcct gcccgtgtca ttatggtatt aaataccctc acgattgcct
     1981 gctttgccat gctttcatag ctcggtatca tggaaagtag aaagctgata ggtaaaaaca
     2041 tagcgaagat aataaaaagt atctggctga acaacatcat acccgtaagc aagaatacaa
     2101 atatcgtaat accaagatta aagaacaaga ggaagaacac catacctaaa cggtttacca
     2161 cctgcggtat cgtcagattg ttgttgtcgt tgtcctcgat ttctgttttc acgacttctt
     2221 ctctggtctt tccgtcctcg tcctctggac ttgccgatac gagagcttcg acacggtctg
     2281 tcccgatttc ctctgcgttg ctgtttccaa actgtaagag tagccacggc tgttgtactt
     2341 gaatagaaaa taggctgtcc cgtatcaagt ctacgctgtc cttgccctcg ctgtcagagt
     2401 tggggagcat gatttttgtt cccaaatcaa gcgaagcggt actgatgtct gatgaaaatt
     2461 catttatctt cttgatgtag tcgggagcgt aggcaataaa cgaagcggac agcacgaaca
     2521 ccaccacaaa gttgataaca gcgtgaagtg ccttgctggt ttcccgtttt atcagtcccg
     2581 tataggcaac atacattccc accactaaga taataagaag caggaaacca acatagaagc
     2641 ccgtagaaga aaagccgttc tcggtaacgc ctgcaagggt ctgtatgctt tttccgatac
     2701 tgtctgccat atcgttaata aaatcaagtt tataggcttc ctgcaccaca tagccagttg
     2761 cattgcttaa ataaaggctt atcgtccaga caaagttggt aatgcagtaa agcccgtact
     2821 gtaccgattt tccgataccg tccagccagt tccacggaag ccacgaccag ctattatcga
     2881 cgaaaaaatc aagctggtag ttggaaagcg gatattttga atatagattt tctgcgttta
     2941 tggtatcgtc cacaagcccc gtcgcatgag ccaccgtccc cagaagtgaa agcaggataa
     3001 gggaaagtgc caccacgaac agagccattt tgagaaagtg aagtatcttc ctttttgtga
     3061 acgcactctg tatcctttct ttcatcactc cacctcattt ctctgtacgg gcggtctagt
     3121 atcaaaggcg tgtagtaatt cttcaaagac tgggtgtatc tgcaccacac cgacacgccc
     3181 gtataagtcc tgcaataaac attgtccgtt ctccaaatca cgcaagcgtt tctggttgtt
     3241 ttcgtcgtcc ttgtcgatac cgaaaaattc taaggtctgc tttatctcgt tgatgtcagt
     3301 agaacgaaat gcaaacttca aaccgatatt gtttttcaga ctttcctttg acacgtcata
     3361 ggcagactgg gtaacgaaat aaacgcctgc ctgcatagct cgtccagcac gaaccagctt
     3421 atttgatagg gtttcgcctt gtgccacatt taagaacgcc cacgcttcgt ccaaatctac
     3481 aatcttaaaa atgcttctgt ccgaatggat aaaatcaagg gcaaaggtac taatcacaat
     3541 cagcatagac accgacaata attcaatggt cgtgtattcc tcaaaagtgg tatctttatc
     3601 tggcagtaca aggtctgcta cttgaatgat attgagctgg ttatcaaggc ttatagcatt
     3661 ttctaccgtc ccgtctgaaa acagcaggtg tgcaaagtcg tagtccgtga agctgtcgat
     3721 atggtctgcg atattgcgtg agatagcggt atcttctttt ctcaattcct caataacgtg
     3781 taacagtcct ctattttcac tctgggtaac agaacgcacc gcttttcgta acacggggaa
     3841 tttttcgccg tccctagagg aaatacccgt aaggaatgtt aagatgtcga ttgccagact
     3901 ttcagcgtct ttcacattct tcataatcac aaatgggtca agaagtcctg cattgtcctt
     3961 gtcgctggta aggtttacga tattgatttc atgggctatc tctgggagcg tttctttcca
     4021 gttgccacgc tcacttttag ggtctaacag aagtgcttga ccgccaaaca gcaccgcata
     4081 atatacaagt agattgttgc agaatgactt tccaccgcca agacttccca caaaagcaga
     4141 agcaagggca ttagtaactg tgccttttac tccctgcgaa gctaaagacg gttgcaggta
     4201 cacatttctt cccgtatcaa cggaatagcc catatagata cccgtgtttt cccctaactg
     4261 ctgggtcgca ccaaaaccaa gcccagccaa gaagtctgac tttacatact gcacatagtc
     4321 attgatatat cgcttgctgg caggtaaaaa ttcagaatga agtcccaaca tatcgccagc
     4381 aggacgcacc agttttacat tgaggtcgtc gtaaaagtcc ttgacttcat cacaacggcg
     4441 tttcagctcg tcaagatcgg gtgcagacac ccgtatgacg tagcttaact tatacatact
     4501 ttctttgctc tggtctaaat cggtttccag ctcgtccacg ctgtctaaag cgtccaccac
     4561 atttgagctg gtttcacttc ctgcttgata ggcgtgatta tccaaatctt tcaattcctt
     4621 tttcttgttg cggactgtcg ttaatgcctt acggttctcc acgatttcta cattcataga
     4681 agtatcaacg gggaatgtga attgctgttg ctggaaatag aagatttcag aggacggaaa
     4741 atccaactcc ccgacaatta cattgacggt aaaataggac acatagcttt ccttgtcctc
     4801 atgttccaat cgtaaatacc gctggctttc ctcaatcaca caccttgtcg gacggataag
     4861 gtcgtagtat tttatcagcg tttctttctt caatttcttc tttggtagct ggtactcata
     4921 atcttcatag gcgataccgt ccctgccgta aagatgttcc atgagatacc caaaatcatg
     4981 gatttccaag cgacgcacct taaagcgacg ggagatttta ttttccagta acttttccat
     5041 tttcatgtaa cggttgattt catcattcgg catggaaaca aaatcattca tcagcgtgtg
     5101 gttcacttca tgaagaaatt ccttgaacgt cagccatgcc gattttttga tgttcttcag
     5161 attgagctgt tcttccgtaa ccatgagctt aaagccaaga aaaaagcggt agtccacttg
     5221 attgtcccca atcattgata ctaacgcttc ggtctgttcg tctatttttt ggactgccac
     5281 ctcacgaagt ttgcctgtta ccagtttctt tgactgctcc tgcatacttc taatggagct
     5341 ttctgtggca atctgcaacg catgaatttt tccctcacgg gactgtgcga ttagctgtcg
     5401 aaaactgtca tgcacgataa atttctgctc tgcggataga aaagaatagt tgtacggtat
     5461 catctcatag taggcgaaca cctcattgtc cttgttccag acaaggttat tgtcaatata
     5521 ttttatcggg aacatagttc acactcctta ctgccgtgat tgtttcattc aatatctgct
     5581 tatgcagttt tacggctttt cctgcataag tgattttagg tcgcaaggca taggttatct
     5641 gtgatttcaa aaagctgtac ggcttctttc cgtcaaaggt tttctgcgac ataaaccaag
     5701 tgagagcaac gggaataccg aagtatttga gaaatgctcc ctcaatcatg gaaagtgggg
     5761 gcatatcccc aaacagaatg atgataaatt ccgtaatcac aaaccatgta atctgtgtaa
     5821 aggtaacggg aaagggtaag ttaaagtcat tgattgcata taagactttt tccacgttcc
     5881 agatacccgt gtagctttta atctttttca agttctttca gcccctttcg taagttcttc
     5941 aaagagaaaa ggacagccat tttcagactg tcccttaaag agaaatacgc tgtcagtagc
     6001 aacaatgctt gttgctgatc ttccagcgtt aatatggtat ctcacagata cctcggcttg
     6061 tttcaatgaa tcgaccaccc atatccaaat ctcgcccgta ggcttcatag tcaatatagt
     6121 tctgtaagtt agctggaatg tccccaagtg cgttcaattc gtctatgtag tagtaggcaa
     6181 catctgtcat ggtttcacaa tcgggataaa aataaatatc gtccttatgt tccacgactt
     6241 cttccagcgt cccatagtgg ctgataaatt cgtccagaca ctctacgata tagtcgggaa
     6301 gttcctctat catttcatac atttcattga gttcttcaat ggaaacatat tcccctatgt
     6361 cgataggaaa gttgtcggta tcatggacag cgtattcttc gtactgttca ttcaagccga
     6421 ttttttcttt tacatcttcc tcgtcaatag ggaaagtgaa ccacgcaccg actaaatatc
     6481 cctcattgta tctgccaaga ttagcaatat aaaccgccat atcatcaatc ataagcacca
     6541 cctcatttcc tattctggca gttcaaagat actgttttcc gttacaagat agcgtttgtt
     6601 ttcaagtaag tgagaagcgt aggcttcaaa atcgaaatag tatgcttgac agtcctcgga
     6661 taaatgtttg aatgtcgggt cgttcatcag cttttgcctt gcaacatcta tcatgctttt
     6721 acagtcgtga taaacggtaa tcacgttcct gcaaatataa agtgcttcta agttctcaaa
     6781 cacggtaaga agttctacat attcttcctg catatcggct gatagctggc ggtacataaa
     6841 atctaattcg ttaagctggt acacgctggt gtgttcgtgg acttcatcag cataaggcaa
     6901 caccttttcg ataatgcggt aatcttgact ttctgcaccg acacctaact tttcttcaaa
     6961 ctctgcaaca tctatcggta agtcaaacca gtatgatgtt gtttcttcgc catttgttct
     7021 tcccgtttcc accagtaccc ttgtttcctg cattgttcct cacgtccttt ctgttgtttc
     7081 atcacatctt ttaaggtctg gtatgacata ccaaacacat atttagaaaa atctttcaac
     7141 tgtttttctt catagtcggc agggttcagc cgtttcattt catgccacac cttacgcttg
     7201 taagagggca ggtcggaaat gtattcacag ccgacttttg cctgcatatc tttaaaaata
     7261 tcgttcattt catcactcca atctgaaatt taggtaagaa aaaagcagtc aatcctaaga
     7321 ctaactgctt tgtgtgtaag tatgaatttg tttaagtgta actgatttac catgtgtact
     7381 cgtaaagttt actgtatgtt tgacttttaa ataaatatat acaatcaatc atttgtaact
     7441 tgccgtcttg ttcaaattta cttattatca gattatcttc atagatatat gctaagaaag
     7501 tatcatactc tgcatagtat ttttctctaa aaagatttga ctctctaaaa tctttttcta
     7561 aagcaattct tatactgtct aaagcatatt taaagttata acaagtatta aaactaaact
     7621 tgtatggaaa aaagaaaaat aagtttttct tgaaagtaag caatttcaca taggtttgta
     7681 tatcaaattc aatcgttcca tactcatatt tctctgtaag acaagaatgt aaactttcac
     7741 aatcataatc ccgcaacgaa gcaaataacc tagttacagt catttgtgta tttggtcttc
     7801 tcggtggaca cttaccaatt acaccatcac agaatttctg tatttgacca gtaagttctt
     7861 ttgaagcatg aagtcttgtt gtgctttcaa gtaatttaaa gtccatttga taattaggtg
     7921 agttacaatc acattctcca tgattttcag atttaggtgg tacatattct gcaaaatttg
     7981 atttttctct aaaatatatt gatttattaa ctaattccaa caagtatttt tcataattac
     8041 aattttcttc atctttaata aaatttttgt ttatgaatac tgcatgtaaa atatccactc
     8101 tcatttcatc tgaaatcata agcactttta ttcctttcaa aaaataattt atcgtaccca
     8161 cattataaca ttctcatatt acagcaacaa taaaattcgt tatgctccaa taatcttatt
     8221 gaacagctct aacagtacat ctttcacacc gccagcgttg aatactaagc cgacagcaat
     8281 aagggcaact accaagaagc caataagttt tgaaaactca cgcttgaacc ctaagtagat
     8341 accgataacc acaatcgcca taagcactaa gctctgtgca tttgataaaa accattgata
     8401 caaattttgt ccgaaattca tttaattgtc ctcgctttct tctaattgtt tcatttcata
     8461 gtcagcttct ttgctgatac tcaaaataca catcaagact acgccgatac ccattcccat
     8521 agataccagc aggaagtctt ttactaattc ccacattttt tatcactcct aactttccac
     8581 caaatctttt gcaggggtcg tctgctgttt gattatcatt tcatgctttt cactcaagat
     8641 tgcctgcttt tcaatcgttt ccatgtagtc tgtaccgttt cccttatcaa tctttttcag
     8701 cattttcaag gtcggtgcta cctgtctttg tacccaacgc aatgtgcggt ctaaggtgta
     8761 aggttctggt tttgtcgtca gtttcagact ttgccgatta tcgccaataa accaagccca
     8821 gcggtcattg agtttccagt catttttccg cttgtctggt tcttcatcaa taaaccgcac
     8881 atactggttg atgatagaaa aagcggtctg ctctgcgtca tagtaggtca gcaaatctcg
     8941 tactgcatag taagcacgtt catttctaag ccgtatctca aaacggttga taatgtcggc
     9001 ttcttccaga ggtgtaccta atttgacata ctgctcatag tccttttcat agatacagaa
     9061 atacacatct gatttcagcg aaccaagata aagggtactc cccatatatt ctctgtcgtc
     9121 ctctctgtgc ttgataagct cgcccgactg gtaatactta tagcttctgg actttccgat
     9181 atattcccgt ttcctgcatt tctccgcaag ctctggaata tccaaaatgc ccgtatggtc
     9241 gttgatagca aggtcgatac gcttcataat gcctccgtct atgagtgcgt ccataagaaa
     9301 gtcataccag cttctttgct gtgccagcag gtaactttca aactgcctgc aaccccgacc
     9361 ctttaactct aaaaggacac ctttttcttc gtcagccgac gtatagataa agatgtcccc
     9421 taatgaataa tgctccgtat aactgtaatg tccgtaatct tcatggagca tataattgat
     9481 attcagtttt aatatgtctt tgatgatgtg ctgtatatcc agcgtgggaa aacgaatttt
     9541 cacataatca aacagcatgg taagcggtgc ttctggattg aaccttgcca gttctttatg
     9601 caaagtttcc agaagttctt ttgacggctt catttttccg ctttctatct tgtttagata
     9661 ttcccttgtg ataccagaag ctaccgccag cctgccttgt gagataccgt aagcaatccg
     9721 cttctcccgt aattctttta tccattgttc ttcattcaga accatacccc caatctgtca
     9781 agtgtgaagt tttttaaggc gacttcacag ccgatttttg ctaggaaacc atgccagaag
     9841 ccttatgttt cctatgtttt tttgtctatt gtctatttgc aaacgtaccc ctctgttaga
     9901 taccgagggg ttttacctgc tggcgtggct tacgccacac cagcaacggc tagtccgagc
     9961 cgtcgccttt cgcttcgcac gtcgccgtcc cgtcctgcct tgcttgtgca agaaaaccga
    10021 ttgtctgcaa aaaatcatgt cctttcggga ctaaaggcgt gtaaaattca cttatgacgc
    10081 ttgttcccac atcacaatag ccacgccctt tgatacgctt ttggaaaaac tgctttttca
    10141 catctgaacc aaacagcata ccgtaaccta actcgctgat acgccccaat cccacacgta
    10201 aattgaagtt atctctgata ccgtccgaga aatattttgc gtctggacgc tggcaggcaa
    10261 cgataaggaa ataacctgct tgtcgtccta acatgacgat tttctttaac tggctaagta
    10321 agcccacact ttcttttgtc cccagcattt caaaaaacgc cacatattca tcaaagataa
    10381 gaaagcaggg tggcagtccc agataggcgt aattttcgcc cgtcttatag ttcgggtgtc
    10441 gtttcatttg ctcgcttcgc tgtaccatgc cctcataaaa agcattgaca cagtctatca
    10501 tttcttcttt ggtgtgatac acgtttccca tgactgttcc caagtctgct aagtccgcat
    10561 ttttcgggtc taagatgtaa aggacagcgt tggtatgcag tagggcttca atgagcgtca
    10621 gaagaaaata tgttttaccg ccaccagtcc caccagcaat cagagcgtga gggagtgcgt
    10681 catattccca gacaagattt ttcatcagtc taagacagcc gttctctgcc cgtacttcat
    10741 caatggtaat gcggttcgct atcatatcat aaagcagggt atattcgata tagccgtcat
    10801 gcagggtctt gtcggtcagc tcacaataca agccactttc caatttatcc tctaaccgta
    10861 aaagctggtc ttgatatttc cccagcgtaa tttcacagcg gatatgaagc agtccctttt
    10921 ccatttgata ataaatcttt ggaaaccaga cgattttttc ccttgatctg ctttgcaggt
    10981 cagtaaaaaa aacgctgtct tgtacggtat cagcttcata ccacttgttt tccagtatca
    11041 ttcgtgccag cttttgacgg tgcagtagct ttttgaaact gtcgtaacag aagcggtaat
    11101 acagaaaagc gaccaatgca caaacaccag tcgctatcag tatggttatg aagttgtaaa
    11161 gggaaagcgt aaagccattg tctaataagc tgaaatgttc ccagtcggta cgcatgagct
    11221 gttttgtatt taatagcagg ataaccgcta cgaacacaaa caggagtgtc cccattgaaa
    11281 agcgatagac aaggttctta tcgctggctc tgatacggtg tcctttgttt aaaatcattc
    11341 tcataaatca atcactcctt tctgggtatg aaaaaagcag tcaatcgtat agactaactg
    11401 ctttgtgtgc atggatatga agttgtcaaa ctggaattta acgttcactc atatattgtt
    11461 ttatttcatc agaagtcaat tttcgtactt ctgtatgtgg atattcttta tactctccaa
    11521 cattaaacac aggtgtcatt tgtttgcatt tcttgtcttt attctttttc aagaatagat
    11581 gtaaatcctc attcccggcg aatattttat aagatactct gcctttactg ttctctatct
    11641 cataaatacc acatgaactt ttcgttgtat aatcggctgt tcgtaaataa agttttgcaa
    11701 tttccaatga cttaaaagga aaattccatc tttgtaatcc tctgtttggg tcatgttcaa
    11761 tacaaatgca acgtccacaa gttccacaaa tatattgagt gtggttttct aagcagtcca
    11821 agggatttag taatggtgtt attctatttt catcagagta acattcttca cacatttttc
    11881 ttttcgctcc ttatcaaatt ctaatttgtc gctttcttca tacccatatt ataacagttc
    11941 cgtatatcct ctacaacgaa aatctattca gcaagtctat tttttcggtt gtccctgcgg
    12001 tgtatggttc tgtgaagtgc ctgtgttctg attgccttta ttcttcagta caatatcttc
    12061 tgcctttaca taccagtcca catctgcacc cgtataggtt tttcgtgata ccgtatcggc
    12121 tacgggattg acaagctcca cgactgcatt gtaaggaaat tcccttaacg gtacttctgg
    12181 cggtacggac acggggataa ttccaccatg cagactgcac ttcaaatcat agatacgctt
    12241 tttaagctgg gtactcggtg tcccgtcctc attttgcaag aacacatcac gcaccgctgt
    12301 aaattttaat tctccaaatg ttttctctct gtcaataaca aaaccgtttg ataatctcat
    12361 aaattcttca ctcctttact tttcttcaac tgctacaata tcatcagcta ctaatacata
    12421 atcggtatgt cccacatccc cgattgcgta ccctctgccg tataacttcg gattgacaag
    12481 ttttactttc tgttcatact tgaaatgctt ttcgccagcc tgcacgggaa tttccaccac
    12541 aacattttcg cctttctgta cgtcagaata aaggttatag cttcgtctgg ctatgacctt
    12601 gcggttgttt ttatcccttt caaagatagg ctcgctttcg cctgcaaatt caagagttcc
    12661 aaaagactgt gccatatctg gcacgacata tttcatttcc atattgtttt acctcgtttc
    12721 tttctatatt tgataagttt atgattgtaa ttttttattc tggcttgtct gccgtttgac
    12781 agtttcggcg gagaacgtga tttttctttc atcataccgt taccgcctta tgattttttc
    12841 attgtacggc gtttgtaaag atatgttcca gccagtccac cagcagatgt aagcaacatc
    12901 acaagaaatg ccatgacatt tgtactgtcg cccgttttag gactgtcgct aggtctgttt
    12961 ggcttttctg gtgtctgcgg tgtttcgagt ttctctggga cttctggttt ttcattaaaa
    13021 gtaatcgtct gtccctcgtc ctcaatatcc ttatgctcgg taactttctt cggctcgtct
    13081 ggattgctta aatcatacaa ttcttcaaaa gttacaagct gtttgccgtc aagggaagta
    13141 gcgtcaaagg taaaggcaac ttccgctttc atagtttcgc tgtcagcggt aaaggtgtaa
    13201 tcactttcca cacgtttccc attgataaga agttctgcat tttcttcttt caacatctgc
    13261 cagcctacaa gtttgtactg tgtaccgact tccaagccct ctaaggttac ggtatcaatg
    13321 attgtaacgt cttttccagc ttcaatctct ttgttgccgt ctttgtcggt agcagtcgta
    13381 tgtatcttga tgatacgttc tgtaatcaat acagtttgtc cgtcgtcctc aatgtctttg
    13441 tgttccgcaa ctttcacggg ttcgtctgga ttgcttaaat cgtataattc ctcgaatgtt
    13501 accagatttt taccgccaag ctcggacgca ttgaatgtat aagaaatttc cactttcatt
    13561 tcttcatcat cagcgataaa agtatagtca ttttccacac gttttccgtc aatgataagc
    13621 tctgcatttt cttcctttaa catctgccag cctttgagct gatattttgt accttttgta
    13681 agcccgtcta atttgacagt atcgacgatt gttacctctt ttcctgcaag gatagtcttt
    13741 tcgctgtctt tgctggtcgc tgtggtatgg atagagattt ctttttcgta ttcatcagtc
    13801 aaagtcccta aatcaatcac aaggttattt cttgatacca cgatttcaaa aggtgggata
    13861 agttcaaagc ctttgttact atcgcaacgc atttcttcaa tgatgtaagt atcataaggc
    13921 aatgcacctt tgctgtcgtc tgcttcagaa gttccaaacc acacaccgtc ctcgctggtc
    13981 ttgcctgcgt tagtattgtg cttatgagaa gtccattcag cagaagtgga gaattgcccg
    14041 ttatcatcag taactacaac atgactttct cccgtcgtct tgcttgtgat cctaaaggga
    14101 acatcagcaa gacgcttgtg tgtgcctgca ccgattttca ctccctcaat atccccacgc
    14161 ttaatctgat tgtagataga gtgggcttcg tcggttaagt ccacgatttt tccattctct
    14221 gtgattgcaa agtcaatcgg cttcgcaccg tctgtcaagt aaccgtcggg agcttcactc
    14281 tcaacgatac ggaatttccc ataaggtaaa aggtcagcag aagtagaagc cacaccctca
    14341 atatctgtac gaattgtctt taccacttca tttttcttat atagcttgcc ctcaaccaaa
    14401 acagaattgt catttaagga aatgacgtca aaagcagtat ctttcaaagt ggcacttcct
    14461 tgtggtttgg tatcgctcgt ttctaaatct cttttctgaa ttttcacacc gccacggata
    14521 accttgtctg atacggaaaa ctggttactt cccgtcaata cagcaaggtc gccgtcctct
    14581 gtaatttgtg taaggtataa gccttttatc tgttcagact tatcgccagc ctgcatatat
    14641 gcaccgtcca gcaagtagcc gtttggagct tttgtttcct caacagatag tgttcctaaa
    14701 ggaagaatcg ctttgccgtc ctgcatatag aagctgtcgc cagatacctt gtaagcgtcc
    14761 gctaattttg tgatgtagtg agttgtcccg tcactgtctg tttcagcgat tgtctttgta
    14821 acccatgtac gagtagcttc ggcagggaga ttgtctttgt tatagaagcc agcataatac
    14881 ttccatgtaa actctgcacc ctctaaagaa gcgtttccct gcggattgtc tttctgcgtt
    14941 tccatatcaa tcttgaaaag ctcaatcaag gtgtccgtta cttttggtgt atctgatacg
    15001 ttcaaggtcg ctgtctttcc agcttcaact tttaaggaat acacggttgt atcaacttta
    15061 tatcctgctg gtgcggataa ttccctaata tagactgtac ctgcttttac ctctacaaca
    15121 tctgtatttc cgtttttatc agtcgtaagg gtggcaagct gtttcgtgca gtccttatca
    15181 gaaaagacac cataggtcgc accagcgatt gagtaattcc cgttaccgtc tgtaatgctg
    15241 gtattactgg aagtcttttg cagttttgca tttccgacat ttaacttcgc ccagaattgt
    15301 cccaattcct gcccctcgcc agagtagata taaccgccac attcatagcg tcctttattt
    15361 tctttcacaa aggctttagc accagcgaaa acttcgtcct gagttgcttt tggtatttca
    15421 tcataagaag ctcgcacgtt gtcgcattgc cagccaagat gtacgctcaa tctctgccag
    15481 acaacacatt gttccaacag atagacttgc ttgtagttca attccttgtg tgcttcgcca
    15541 tactgtttga catactctaa ggataacgcc acatctgaaa tctggtcggc actcatacgg
    15601 gtgcttgcgt cagctctggt cttgtagcca ttcttaaaat ctgtattgat gtctatacag
    15661 taagcgtcct cgccctcaac agtcaaatgt ccctcgttaa aggtagaacc aatcgaaccg
    15721 tcattcatta ctttttctac gataccgaca cgttcctctg attccgtcca gtattgcttg
    15781 ctttctgcat ggacggcggt actcggtaaa gcagtaacga cagtcgcaaa ggcaagtact
    15841 cccgtacaca atcgtttcaa tatctttttc ataattctaa atgctccttt cattttgggt
    15901 aaaaaaatag acgctcaata ttgaacgtct tacattgaaa agggatactt tttacaagta
    15961 cccctcaaat ctaatattta attgtttgtg ttcccacact ctatatcaac aactactgaa
    16021 acattgatat agttttttat ctctttattt ctgattttat cgccctcgct cgtaaagact
    16081 atatgcttaa aaaagtgcct aaaattaagg atttctacat agttcgtctt tgtagcaaca
    16141 cacaagtctc tatatgtgta gaatgaggga acatatctac aggttgtgcc tcaacacatt
    16201 taaatccgtg aacatttaga taatctaaat cccttgctaa agtagaagga ttacaagaaa
    16261 catagacaat tctttccgga ttcattgaaa caatagtctc taataccttt tcgtcacatc
    16321 cctttctagg tgggtctaca acgactacat tggcagtctt accctctcta tacatcttag
    16381 gtacaatttc ctctgccttt ccaacataaa attcagcatt atgtatattg tttaatttag
    16441 catttatctt agcatctttt atggcatcat ctataatttc tattccataa acctttttag
    16501 ccttttgtgc caaaaataaa gatattgtac ctatcccaca gtaaatatca aaaacagtat
    16561 cagtatcatt taaatttgca tattcaagag ccttatcata caatacttta gtttggacag
    16621 ggtttacttg gaaaaatgat aatggtgaaa tctcaaacac taagtctcct atataatcgt
    16681 ttatttttcc atctccatat ataactatat tttctcttcc tagaatagca ttcgtatctt
    16741 tttgatttac atttacaacc aaagtcttaa atccaggtat attttcctta agcactgaag
    16801 ctaattcttt aagatatgga agtttgtttc catttgctac taatacaacc attacttctt
    16861 tagttgtaaa acctattttt gttactaaat gcctaatcaa ccccgtgtga gtcttttcat
    16921 catatatgct taccttatag gcccttatat atgtttttat tattttaaca attttatcat
    16981 ttatctcatg ttgtattacg catttttctg tcgatattac atcatgagtc ttctttttat
    17041 agaagccaat aattggaata ccattattct tttgtattgg aaattgtgct ttatttctat
    17101 atctatatgg attatccatt cctatagtgt catgaacttc tacatcctct aacttaccta
    17161 ttctcgaaag tacttgttta acttcatttg ttttcatttc aagttgttta ttataatcta
    17221 agtcttgtat ctgacaccct ccacaatccc taagattgtc actacacact cttttcaccc
    17281 tgaatggaga cttttctatt atctcaacaa tatctcccac agcataattt ttctttactt
    17341 tattaatcct aactttaact ttatcatgta ttaatccgcc ctctacaaaa actgtaatac
    17401 cattgtactt tcctactcct actccgcctt gaccaatgtc tactatatct acaatataca
    17461 tctcgttttt cgaaagcata aatcaccctc caatcaatct attatattta aatctttaca
    17521 aacttttttc tttaaattta tattaaaata ttgctattaa ttaaactata ttttttacta
    17581 agctacaata ttttatttta ccaaaaaaaa aggagcttat aaagcccctt agtaaataaa
    17641 tttattattt ttcttataaa actctagttg aacctctata taccacgcct cttgatgcat
    17701 ccacagttac aacttctcca tcttttacta gatttgttat atcagctgca gacacaataa
    17761 ctggtatatc taaatttata cctacaatag ctgtatgtga tgtcattcca ccattctcag
    17821 ttactacagc tcctgctttt tctatataat tgttcatttc agcatcagta gcagtagaaa
    17881 caagtatgtc gccttcatta aagtctacac aagcatttcc attctttatt atacgaactt
    17941 taccttctac tgtttgagta cctacaccta taccttgcac gatttcttcg cttattacat
    18001 gaacctttat taaattagta gttccactta ctcctactgg tactccagca gttataacta
    18061 ctaattcccc attatctata taatcagctt gtctagctgc ctcaattgcc ttttctataa
    18121 cttcatctgt atttcctgca acagaagact taactggata tactccccat gtaagagcta
    18181 atttattcat agtcttttca ttatttgtag tagctattat tggagatttt ggtctgaatt
    18241 tagataccat tctagcagta tatcctgaag aagtagatgt tattatagct gatgcattta
    18301 aatctactgc tgttgtacaa gttgcatggc ttattgcatc tgttacagta acattgttag
    18361 taccattttc ctttagtaat ctatcataat ctaaagtctc ttctgttctt tttgctattg
    18421 tagccatcat ttttactgcc tctacaggat attttccagc agcagtttct ccagataaca
    18481 ttatcgcatc tgttccatca tatatagcat tggcaacgtc tgtaacttct gctcttgttg
    18541 gtcttggatt tcttatcata gaatcaagca tttgagtggc agtaacaact ggctttgcta
    18601 attcattaca tttttttatc atcatttttt gaactattgg catctcttca gttggtatct
    18661 caacacctaa gtcacctcta gccaccatta taccatcaga aacttttaat atttcatcta
    18721 ggttctctac accttcttgg ttttctattt tagatattat ttgtatatca gttgcattat
    18781 tgttctctaa tacttctctt attgctagta catcagatgc ctttcttaca aaagaagcag
    18841 ctatataatc tattccttga cttattccaa actctatatc acttatatct ttaggagtta
    18901 ttgctggtaa atttatctta acaccaggta catttactcc tttatgattt ttaactattc
    18961 ctgaattttc aacaacacaa actatatctt ctccatttat ttcttttact cttaagccaa
    19021 ctaaaccatc atctattagt atagaatctc ctgatttaac gtcttcagca agacctttat
    19081 agcttactgt acacatttct ttagtcccca taatatcttt catagttata gtgaactttt
    19141 gcccttcttc taaaaatacc tctgggtctt caaagttacc tgttctaatt tctggacctt
    19201 tagtatctaa taatatagca actggcttat ttaatttttc tctaactttc tttgccatat
    19261 caattctttc tttatgttct tcatgcgaac cgtgagaaaa gttaaatcta cacacattta
    19321 gaccattttg cataagttga gttaaaactt cctcactttg tgatgctgga cctagtgtac
    19381 aaacaatttt tgtcttcttt acattattta acatatttgc tcctcctgag atattattcc
    19441 acagatttat ttttaatact aaaacttata tagataatat ttttgccatc tcataagctt
    19501 ctttatcaaa tactttcttt tgagctaaag cttcacttat ttctaaatca attattttgt
    19561 tttcttttat tcctacaact ctagctgatt ttccatctaa taataattct actgctctta
    19621 ctccaagtct acttgctagt attctatcag atacagttgg gcttcctcct ctttggacat
    19681 gccccaatac agtgactctt aaatctgctc ctgactcttt tttaagttcc ttttctaaat
    19741 cactagcact tccgactcct tctgctaaaa cgattatact atgtctctta cctctttttt
    19801 gagtagtctt taatcttaga gcaacttcat ctactgtaat ttctacttct ggtactatta
    19861 tagtttccgc tcctcctgca agacctgcat ataaagctaa atctccacag tgtcttccca
    19921 taacttctac tatgttaact ctttcatgag atgatgatgt atctcttatc ttacctatag
    19981 catctataat agtattcatt gcagtatcaa atcctatagt ataatctgta tatgctaaat
    20041 cgttatctat agttcctggt atgcctatag ctgggaatcc taagtcacta agtttttggg
    20101 ctcctgcaaa tgaaccatct ccaccaatta caactaagca atcaatttta tactttttaa
    20161 gaatttttac tgcttttaat ctaccttctt ctgttttaaa ttcttcacat ctggaagatt
    20221 ttaatatagt tcctcccctg tgtataatat ctccaacaga agataagttc atttcagtta
    20281 aatcttcttc tagtaatccc ttatatcctc tattgattcc atatactttg catccataat
    20341 atattgctga tcttacaacc gctctaatcg cagcattcat tcctggagca tctcctccac
    20401 ttgttagtaa tcctatagtt ttcatttttt acctccttgg attttatgtc aattttttat
    20461 tataatcatt gttattgtat tctaatggac tttttttgtc cctatatcac tttttaatcc
    20521 catcaagact aaaaaaatta aatgtcctta aatgtccatt ttgtgatatt atatcacaaa
    20581 acggacaata tttttcatat tattataata tatttagatt tttatataaa aaatcaagct
    20641 tttactttta tgttatcttt aggaagtaac tcttctaagt tatttataaa ttcatcacat
    20701 atacaagtca atgccccggt cattttatat gctttacgtt taccatttat attttcatcc
    20761 atagggtata agtatatact gtcattacct ggatattgtt tggctatttc tacaatatta
    20821 ttagttgtat cagtatcaga catactatct attttcaaat acaatttttt accactttct
    20881 ggcttaatat tttctacatt tctagttctt cctgaattgt agtttctatc tgcaaagtca
    20941 gtatcatcat ttatatcttt tatctctcta gctataagtt ttgcattttc gccttctttt
    21001 atacttaatg ttccttttac atatattata ttatcttcat tcaaaatgac attatacttt
    21061 tgtaataatt gaggaaaaac tattatttct atagcaccat acaaatcttc taattcaata
    21121 aatgccataa tttcatttcg ttttgttgtt ttaatagttt tagttacaat cattccaccc
    21181 attataacat ctgtttcatt catttgtagg tatgactctt cttcctcttt taatgaattt
    21241 aattttccat tatccataga tgttttttct tgtagttctt tttcaaattg tgataaagga
    21301 tgtccactta catacattcc caagacttct ttttcaaggt taagtctctc tctttcttcg
    21361 tactcttgcc ttttaggcaa actgtatatt tgttgaaaat cactatcttc ctcactatct
    21421 ccaaaagcat caaataaaga aatttgacct tgaacatttt tctttctatc catagataca
    21481 ctttctaata aactttcata accagccatc agactagctc tattttcact tatattatca
    21541 aaagctccac attttattag actttctatt actctcttat ttgtatcttt ttggtctaat
    21601 cttttgacta aatctactaa atctacaaat aatccattag attctctttc agcgattata
    21661 ttgtttatta tatttacgcc aacatttttt acggctgcta gaccaaatct tatattgtcg
    21721 ccttcaactg aaaactttga aaaacttttg tttatatctg gaggtaatac atctatttct
    21781 agtgctttac attctcttat atactcaact accttatcag tatttcccat tacactggtt
    21841 atcaatgctg ccataaattc aactggataa tatgttttta gataagctgt ctcatatgct
    21901 aaaaccccat atgcagctgc atgtgattta ttaaatgcat atctagcgaa gtctatcata
    21961 tcatcaaata ttttattagc tatttcctct tctacaccat ttctgataca ccctgcaatt
    22021 tctatattac catcttcatc aaatttacca tgaataaaat attgtctttc ttcttccatc
    22081 acgtccatct ttttcttact catggctctt ctaactaggt cacttcgtcc gtaactataa
    22141 ccacctaaat ctctaacgac ctgcataacc tgctcttgat ataccaaaca accatatgta
    22201 acttccatta taggctttaa tttttcatgt agatatgtta catcttttgg gttgctttta
    22261 ttttttatat atgcaggtat agaatccatt ggacctggtc taaataggga tattcccgct
    22321 acaatatctt caaaattgtc tggttttagc tgtttcataa aacttctcat gccagcactc
    22381 tccaattgaa acacccctag tgtatttcct gatgaaagca tttcatatac tttcgggtcg
    22441 tcataatcca tctttgaaaa gtctattttt tctgtgtaat tttttataac tctatttttt
    22501 tctattaaat caagagtatc tcttataact gtaagagttc ttagcccaag aaaatccatc
    22561 tttaaaagcc caagttcttc aagtgttgtc atagtaaact gtgttgtgat tgcatcttga
    22621 tgtttgtaca aaggtacata ttcatctacg ggattttttg atataacaac tcctgctgca
    22681 tgagtagaag catgtcttag cattccctct atctgttttg atatatttat tatttctttt
    22741 gtttccgggt cttggtcata caattcttta agattaggat ttgtatctag ggccttatct
    22801 attgtcattc ctaaagcaaa tggtatttcc ttggctacct tgtcaacttt attgtagcct
    22861 atatccaata cccttccaac atctcttatt gcagcttttg cacccattgt tccaaaagtt
    22921 attatctggg ctacatggtc atcaccatat tttctcttta cataatctat aacttcttct
    22981 cttctctcat aacaaaaatc tatatctata tctggcatgg atatacgttc tggatttaaa
    23041 aaacgctcaa ataatagaga gtactttatt gggtctatat cagttatttt aagtgtatat
    23101 gctactatcg aaccagccgc actacctcta cctggtccta ctataatatt attttcttta
    23161 gaaaaattta taaaatccca aacaattaaa aaatactcta cataacccat tttttcaatt
    23221 acatctaatt cataatttaa tctctctagt atctcattac taggattatc atatctctct
    23281 tttaatccat taaaacacaa ctcccttaaa taagtttctg gtgtatatcc ttctggaaca
    23341 tcatacttag gaagatgtat agtattaaaa tcaaactcca cattacacat atcagctatt
    23401 ttcacagtat tgtcaatagc ctctaatgca tatggaaata attcttccat ttcttctctt
    23461 gattttaaat aaaactcatc actaccaaat ctcattctat ttgggtcatt taatgttttt
    23521 cccatttgaa tacacataag tacatcatgt attttcgaat cttctttatt tacatagtga
    23581 acatcattag tagcaactag tggtatacca gtctcttttg ataattttac taaaccagag
    23641 tttacttctt tttgctcagg taaattgtga tcttgaatct ccaaaaagaa attttcttct
    23701 ccaaatatgt ctctgtagtc aagagcaaat tgttttgctt tttcataatt tctattcatt
    23761 aaagcacgtg ctacatctcc tgcaaggcat gcagaaagtg ctataatccc tttgttatgt
    23821 tttttaagtt cttccatgtc aactctaggc ttatagtaaa aaccttctac gtatgaagtt
    23881 gaaactattt ttattaaatt tttatatcct tccatattct tagctaacag aactaaatgt
    23941 ccttgatatt tgtcatagtt tgggtcttta tctctcaatc cacgtgcagc tgtatagact
    24001 tcacacccta taattggctt tattccttct tttatagctg ttttgtagaa atctataaca
    24061 ccaaacatac aaccatggtc agttatagct attgagctca tgtttagttc cttagccctt
    24121 tttataagtt ttttcaccct cgaaaaccca tcaagtaaac tatactcagt atgtacatga
    24181 agatgagcaa aatctttgtt cattaaatca cctcctgctt tataatttta attttaccac
    24241 aaatatatgt cctaaaacaa aaacagagac ttttatctca aaataggaat taacttcaac
    24301 atataccatt atatgttata aattaatgct tattctgaga ttgtcccctc aatttattgc
    24361 tatgattttt ttttcaaatc tgttgcaatt tcttctattt ttctaagtct atgattgact
    24421 ccagattttc ctattggtgg ttctaacatc tctccaagtt ctttgagagt catatcctca
    24481 tactcgattc taatcttagc tatctcttgt aaattttctg gcaaactgct aattcctata
    24541 gtctcttgta tgagcttaat attttctact tgtcttactg ctgcatttac tgttttagaa
    24601 aggttagctg tctcacaatt tactatcctg tttacattat ttctcatttc ttttactatt
    24661 ttagtatttt gaagacttaa aagagcgtgg tgtcctccta taatactcaa caaatctgat
    24721 atttgttcac tctctttgat ataaacaacg tgattatttt ttctagccac aattttacta
    24781 ttaaaaccta aagagtttat aagttctttt agagaatctg caaactcctc attattagtt
    24841 acaaattcca tatgatagtt cttttctggg tcgcttatag aacctccacc aagaaaagct
    24901 cctcttatga aagctctctt acatccttca tgcttaatta agttctctga aattttgttt
    24961 atagtaaaaa atccttcttt atcttcaagt atacctaact tttttaataa catttctgaa
    25021 cccatttcgc ttgttaccat taatacataa ctgttatttc tctttagcat ttgattctta
    25081 tttactgaga ttgtagtatt gatattaaaa ttctttttta ataatttaaa tactttacgg
    25141 gctattgaat ttaactctgt tgttatcttt agatttagct tcttatatcc aacaatttgt
    25201 atacttcccc ctgatttaac tagagctgac aattcagcta tattacaaca ttcattccca
    25261 gatataactc ttgctagttc atttttggtt tcagttgaaa aagacatatc tatcacctat
    25321 taactaattt ttctttaacc ttttttttat ttcttgtaag gcaaccttta ttatctcctt
    25381 ttcatcatca tacctagcta agtctactac tctatctagg tggataaatt tagcatcaat
    25441 aaagccttcc tctttttgtg gaatagtatc catattcttt gcttgcatca aataccaaaa
    25501 aactgtttta tgaactgctc tattttcatc ccagttttct ttgaatgtat agtgtatttc
    25561 acctaaatat ttcaatatat ctgcttttac tccagtttct tcacttactt cacgtaatgc
    25621 tgcctcttgg ttattttccc cttcttctac ttttcccttg ggtaacaccc aatctccatt
    25681 gaacttcctt agtaaaagaa tcgtattgcc aaacaggact acaccaccag cactaacctc
    25741 ttctctcatc gatgttcact ccttaaaact aacataatga taaggatttc tacacaattt
    25801 tttaaaatcc ttttttacta acttttgttg taattatgat ttagggctaa ctcaataact
    25861 atgttagata tatactttgc atcatgtctt atataattat tttttatttc aattaaattc
    25921 ttctctacag tttttatacc catctctttt aatttatctt tttgcttttt atctaacaag
    25981 acttgctctg ctccatcttg tttatataag tcaagcattt cttctggcaa tatctcatta
    26041 tttgcgatta cataatctat taaattctca tcagtgtgtt ttactatagc attaacatgc
    26101 tctaatacat tgtacccttc tgtctcacct ggttgagtca ttatatttgc tatatatact
    26161 tttggagcta cagacgattt aatagcgtct acaatcccct ctacaagtaa attgggtatt
    26221 atacttgtat ataaacttcc aggacccata attataatgt cagcatcata aatagatgca
    26281 ataacatcct ttaatggttt tacatctttt ggatttaaaa aaattttatc aatagagctt
    26341 ttttgaattt ttgactcttc aggtatacta gattctccat ttattatatt gccattttct
    26401 aatttggcaa ctaaatttac atcttccaaa gttactggta aaactcttcc tgttatagca
    26461 aaaatttcac ttaatttata tacagctttt tcaaagtttc catacaaccc attcatagca
    26521 gccaaaaaca aatttccaaa actctgacct tttaaaagcc cttctgtaaa cctatattgc
    26581 ataacttcat tcattgttgg ctctatattt gcaagtgcta ataaacagtt tcttatgtct
    26641 cctggtggga gcattcctaa gtcttctctc aatacacctg aacctccacc atcatcagca
    26701 acagttacaa ttgcagttat attttgcgta gtgtgtttaa gacctcttaa aaataccgat
    26761 tgaccagtac ctccacctat cacaaccaca tttggttctc tattttcaac aatagacctt
    26821 tgaaatagtt ttactttttc attttttgct tttttatata taaataacga tacaattaat
    26881 gcaatccaac ctaaaattcc tacaataata agtaatttag ccatgtttgc ctccattatc
    26941 gcaacataga gtctctatgt ttctttacaa ctctatatcc cttattagat aaatcctccg
    27001 ctataagatt tgttatagta actgacctat gtcttccacc tgtgcatcca acaccaatta
    27061 ctagatgttg ttttccttcc tctatatatt gaggtactaa aaaatgaatc atatctaaaa
    27121 gtttcacata aaattcttca ctgattttag aattcataac ataatctcta acttctttat
    27181 catcaccagt tttagctctt aactcttcaa cataataggg atttggaaga aatcttacat
    27241 caaatactaa atctgcatca gcaagtatac catgtttaaa tccaaaagat actacagaaa
    27301 tagttaggtt tggattatct tcacctgaag aatatatttt ctttatttct tccttaaggt
    27361 ctttaggttt catgttagtt gtatcaatta tacatgtaga taactccttt aaaggttcca
    27421 ttatctttct ttccattttt ataccttctg gaatctgcat atcttttgcc aatggatgat
    27481 ttcttcttgt cattttatat ctctttaaca atacatcatc attacaatcc aaatatacca
    27541 tttcatattc ataattttct ttttctaaat aatttaagct ttcatgaagc gcttcaaaaa
    27601 actttctccc tcttatatct atccctagag caactttatc aatacttgaa tttggttgat
    27661 aacataattc tgcaaattta gttatcaaag caggaggtag attatctaca caataaaatc
    27721 ccatatcctc taaagctctc attgtctcac tttttccaga tcctgaaaga cctgtaacta
    27781 taacaaattt cattgctaac tcctcccttt tatcttatgt ttattatatt ataatgtttt
    27841 ctactaacga tatatccaga ttagagtcag atatagtctt catagctagt tcaaaatcac
    27901 caactctttc tggacaatgt gcatctgagc ctattataaa tttattttct agccctttta
    27961 ttttatttaa ttgatttaca ttcaaaaaag aatggctact atttatctca agtctagtat
    28021 ttgtcttttt tgctgtcttt gctacttctt ctatatacac atcgccttta tctcctggat
    28081 gagtaattat aaaaatatca ttattactta tcgcatttat tacagctttt gtattatatt
    28141 cttttgcttt ttcagtttta aaaatatagt tattacaatg atttaataag cttcttaatg
    28201 atgttggagt agaaccaaaa tgatatccag ccataatata atcaaaatct ctaataaatt
    28261 tatcatccat atctatattt cctttatcat ctaatatgtt acactccata cctaaaagta
    28321 tatttatatc agtgtactta tcatttaaat aatctacttc ttctctcatt ttgagaatat
    28381 cttttttctt tacaccatat gcaatatgtt tataactatg gtcagatatt cctattgttt
    28441 ttattccttt agaaatggct acctttacat tatcctcaat agtccctttg ccatgactat
    28501 ataatgtatg tgtatgataa tcagccagta ttttcatttg ttaatcctct ccaagtattt
    28561 tgacttcttc ttccaacata accccaaact tggcatatac agcacttttt ataacatata
    28621 ttaaatccaa aatatcttta gcacttgctt ctccttgatt gactacaaat ccacaatgct
    28681 tctcagatac ttgtgctcct cttaaagtaa gacctctcaa tccagtttct tctattaatt
    28741 tacctgcaaa atatcctgtt ggtctcttaa atgtacttcc tgcactagca aaatttaatg
    28801 gctgtttagt tattcttctc gtcgctaaat catccattaa actttttatt tcatcataat
    28861 ttccttcttg taattccaat tctgctgata aagctatata tccgtttttc gatattatac
    28921 tttttctata cccaaattcc atctgttcat ttgataattc aaatatattg ccttccatat
    28981 ccatcagtct tactgacttc actatgtcct tcatttcacc accatatgct ccagcattca
    29041 tggctaatgc tcctcccaat gtgccaggta ttcctgatgc aaactcaaac ccttttaatt
    29101 cttgcttttg gagagctttc cctaaaactg ataataaagc tcctgactga gcagtcatcc
    29161 tagtatcatt tatctcataa gaattgaaat tatcagctag ttctattacc actcctctta
    29221 ttccaccatc tttaattaaa atgtttgaac catttccaat tattaaatac ggaatagatt
    29281 cttcttttac caacttaagt acatttttta attgttcttc agttctaggt ctaactaata
    29341 tatctgctgg tcctcctacc ctaaatgaaa tatgtttttt cataggttca tcgactttta
    29401 tatcctcttt acttaatata tttaataaat ttatatatat atcttgatta ttcattgtat
    29461 ttcctcccaa attatactga actttagctg tcattattat ttaaattaca ttagtatata
    29521 gtattaacta tattcaataa tatatgattt tgcttatcat atggatttta tattttaagc
    29581 ttcttaatta caaactatat tcaataaata tataataaag taaaaaaaaa aaaataacaa
    29641 catataaaag aatataaata atttaaaaaa agcatcaata tcactgatat caatgcttta
    29701 aaaggcaatt taattcgtat tatttatcta actatttttg acatagcaaa gataataaat
    29761 ccctccaact actatggaac ctccaacgat atttccaagc gttacaaata ccaaattagt
    29821 tataaattga accatagtta tatttgctcc taatgccatt cccattggta taaaaaacat
    29881 gtttgcaaca ctatgttgga atccacataa tacaaatagc attattactg tccaacatga
    29941 agccaatttt gacattatat cttgagatgc tgtagccatc catacagcta aaacgactag
    30001 aatattacaa agtattgctt ttaaaaacat tgatgctata gttgaattaa ccttagcttc
    30061 tgccacagct atagcttttg tagctgcatc tcctgtcaat gtagaagaat aaaacacaag
    30121 tgcaactagc aaaattgaac ctactaaatt tgcaaaataa accagtcccc agtttcttaa
    30181 catttttcct aatgttattt ttttgtccat aagtgcaata gccattaagg tgtttccagt
    30241 gaataattct gctcctgcca ctactacaag cataagacct actggaaaga ccatagcact
    30301 taaaaagcta gctacccctg ggtctatctt gccaaaagtt tgactaacta tcatactccc
    30361 caatgcccca aaacctataa atgctcctcc aaataaacct aatagaattg cgttttgtgt
    30421 cgaaatattt gcttttttaa tgcctgcact tatagttgct tgtgcagttt ccccaggtgt
    30481 caagaatcct ttactcataa atccttcccc cttaaaaatt atactttccc ttgtcttttt
    30541 atggcaataa ttatataata acaatatgat atataataaa ttttattaac ataaaaaata
    30601 tactatatac ttatatatac ttgtctcata taattatact atttattata taacaacttt
    30661 gttaatttcg
//
LOCUS       DACULQ010000026        30441 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.026, whole genome shotgun sequence.
ACCESSION   DACULQ010000026 DACULQ010000000
VERSION     DACULQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.026"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            32..1144
                     /locus_tag="JA154_04990"
     CDS             32..1144
                     /locus_tag="JA154_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HAT7995214.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLAYLLLKKLNVGSTATIITFLCIATFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENL
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1395..3422
                     /locus_tag="JA154_04995"
     CDS             1395..3422
                     /locus_tag="JA154_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT7995215.1"
                     /translation="MMKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININL
                     QRKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNK
                     DPEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNE
                     YMRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYD
                     APILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGV
                     DRHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQ
                     GTYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIK
                     TFYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAE
                     TVYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGL
                     NGGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSI
                     HYNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKD
                     YLYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="JA154_05000"
     CDS             3642..4328
                     /locus_tag="JA154_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAT7995216.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="JA154_05005"
     CDS             4423..5478
                     /locus_tag="JA154_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HAT7995217.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7149
                     /locus_tag="JA154_05010"
     CDS             5593..7149
                     /locus_tag="JA154_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAT7995218.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7195..8901
                     /locus_tag="JA154_05015"
     CDS             7195..8901
                     /locus_tag="JA154_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAT7995219.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8923..9996
                     /locus_tag="JA154_05020"
     CDS             8923..9996
                     /locus_tag="JA154_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HAT7995220.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10008..10730
                     /locus_tag="JA154_05025"
     CDS             10008..10730
                     /locus_tag="JA154_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAT7995221.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LVCGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10746..11903
                     /locus_tag="JA154_05030"
     CDS             10746..11903
                     /locus_tag="JA154_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HAT7995222.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRILLFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPVILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11906..12745
                     /locus_tag="JA154_05035"
     CDS             11906..12745
                     /locus_tag="JA154_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT7995223.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12745..13935
                     /locus_tag="JA154_05040"
     CDS             12745..13935
                     /locus_tag="JA154_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphotransferase"
                     /protein_id="HAT7995224.1"
                     /translation="MRMVFMDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDI
                     YNDNSKFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIV
                     FSHAPSADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKS
                     YNIVTASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWI
                     KFSSGKFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGK
                     RIVFLDKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYID
                     LKSEMFGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVL
                     SKIK"
     gene            13964..14713
                     /locus_tag="JA154_05045"
     CDS             13964..14713
                     /locus_tag="JA154_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT7995225.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14735..15643
                     /locus_tag="JA154_05050"
     CDS             14735..15643
                     /locus_tag="JA154_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT7995226.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15691..16443
                     /locus_tag="JA154_05055"
     CDS             15691..16443
                     /locus_tag="JA154_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT7995227.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16469..17764
                     /locus_tag="JA154_05060"
     CDS             16469..17764
                     /locus_tag="JA154_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAT7995228.1"
                     /translation="MKIAIAGTGYVGLVTGVCLAEVGNENVVCVDVDEQKVKSMKLGI
                     APIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVGTPERSDGSANLDYIKV
                     VAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNDVKVELASNPEFLSQGTAV
                     KDTLHASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAEMIKYASNNFLALKISF
                     MNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGSCFPKDTKALHWLSEHN
                     GYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGLTFKPDTDDIREAPSIP
                     NIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIEDAEICFIMTEWKDVLN
                     YDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            17770..18861
                     /locus_tag="JA154_05065"
     CDS             17770..18861
                     /locus_tag="JA154_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAT7995229.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18945..20858
                     /locus_tag="JA154_05070"
     CDS             18945..20858
                     /locus_tag="JA154_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAT7995230.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKHN
                     VDEIIFSIANIEKRRKKEIIDVCKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21057..21764
                     /locus_tag="JA154_05075"
     CDS             21057..21764
                     /locus_tag="JA154_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HAT7995231.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22066..24177
                     /locus_tag="JA154_05080"
     CDS             22066..24177
                     /locus_tag="JA154_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HAT7995232.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGSLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24375..25340)
                     /locus_tag="JA154_05085"
     CDS             complement(24375..25340)
                     /locus_tag="JA154_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAT7995233.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWSKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25625..26581)
                     /locus_tag="JA154_05090"
     CDS             complement(25625..26581)
                     /locus_tag="JA154_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAT7995234.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GHGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26949..27737
                     /locus_tag="JA154_05095"
     CDS             26949..27737
                     /locus_tag="JA154_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAT7995235.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27860..28330)
                     /locus_tag="JA154_05100"
     CDS             complement(27860..28330)
                     /locus_tag="JA154_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995236.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28469..29113
                     /locus_tag="JA154_05105"
     CDS             28469..29113
                     /locus_tag="JA154_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAT7995237.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29156..30061
                     /locus_tag="JA154_05110"
     CDS             29156..30061
                     /locus_tag="JA154_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT7995238.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        12067 a         3305 c         5403 g         9666 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagcat atttactgct
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatgta tagctacatt
      481 tataatagtt gggtactaca tcttagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa aggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acctaggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cccattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatataca gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaat gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgacgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtgactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agattctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gattcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aaaacttatg
     2701 tttctgcata ccataataca aatttatcag aaaaaacagc agaaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcaggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaatagaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacag ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattga gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggtcctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagaatt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 atgaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gcaaaaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agagtttatt gcagagattg attctaactt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctagattag actttggtag tagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattggtct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaatgt atggatactg ccagcaggag cattattggc tatggcgagt
     6181 cagctattgt ttcaactgcc atttgcgttt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtctat aaaagagtta gtcaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gaccgattat tggcaaccac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagcac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttagaatatt attccaaagg
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacac caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atcaaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagataga taattacctt gaaattttaa attagagtca gaaaggatga aaatatggat
     7201 tataagaata attatgagat gtggttaaat tctccttatt ttgatgaaca aacgaaaaat
     7261 gagctattaa gtataaaaga tgatgaaaaa gaaatacaag atagatttta taaaaattta
     7321 gagtttggaa caggtggttt aagaggtata ataggagcgg gaactaatag aatcaatatt
     7381 tatactgtta gacgtgcgac actgggcgtt ttaaattata tcatgaaaac tcaaggagaa
     7441 gaaggtaagc aaaagggaat agttatagca catgatagta gatacatgtc tagggagttt
     7501 tgtatagaag tagcaaagac tttaagtgct tatggtgtaa aggcttatat ttttgaagaa
     7561 ttaaagccta caccagaact ttcttttgct gttagatatt taaagtgtgc tatgggaata
     7621 gtaataacag caagtcataa tcctaaagaa tacaatggat ataaagtata tgactcagat
     7681 ggtggtcaaa tctgtataga tatggcaaat gatataatag cagaagttaa taagattgat
     7741 gattacagta ctattaaaag tatagatttt gaagaagctt tatctaaaaa tttaataact
     7801 atattagata atgaagtaga tgatgagttt ataaaagctg ttaagaaaca agttttaaga
     7861 caaaatataa tagatgaata cgggaaaaaa ttaaaaataa tatatacacc tattcatggt
     7921 acaggaaata agccagtaag aaaagtctta aatgaatgtg gttttgaaaa tgtaatggta
     7981 gttaaggagc aagaattgcc agattctaat ttttcaacag taaagtatcc taatcctgaa
     8041 gaaaagtcag tgtttaatat agctattgaa atggcaaaga ataatggcac tgatttgata
     8101 ataggaacag accctgattg tgatagagta ggtatcgttg taaaagattc aagtggtgaa
     8161 tatgtagtct taaatggaaa tcaagtcggt tcacttttag ttagatatat attagagagt
     8221 ttagttgaag aaaataaact tcctaaaaac aatcctacaa taataaaaac tatagttaca
     8281 tcagaactag gtgcaaaaat agcaaaggct tataatgtag attgtttaaa tactttaaca
     8341 ggatttaaat ttataggaga aaaaataaaa gcgtttgaag aaagcaatga tagaagtttt
     8401 ataatgggat atgaggaaag ctatggttac ttaattggaa ctcatgctag agataaggat
     8461 ggtgttgtat cttcacttat gatatgtgaa atggcagcat actattcttc aaaaggtatg
     8521 aacttgtatg aagctttgat agatacatat aataaatttg gatactacaa agaggactta
     8581 aagtcagtaa ctttaaaagg catagatgga ataaaaaaaa taaaagagat gatgctttac
     8641 tttagaagtg ttaaaataga taatgtggct gatgtaaaag tagataaaat tttagactat
     8701 aaagatggtg ttgatgattt acctaagtca gatgtactta aatttttact tgaagatggc
     8761 tcttggatag ctataagacc ttcaggtaca gaacctaaaa ttaaatttta ctttggagca
     8821 aattcagaca atcaagaaga tgtagaattt aagttgaaca atttaatatc ttatatatta
     8881 aatgtagtgg attctatata aaaagctaag gggagataaa caatgaaagt atacaatgtc
     8941 ataatggctg gaggaggagg aactcgtttc tggccactta gtagacaaga agtacctaaa
     9001 cagcttataa atttaagtgg agaagatgct ttaataaacg aaactataaa cagaatagat
     9061 tctctagcaa aaaaagatga tttatttata gtaaccaatg agaagcaatt ggaagcttta
     9121 aaggatatag taaaagataa atgtttggat agtaatatat tgccagagcc atgtgccaga
     9181 aatacagcag cagctatagg ctttgcagca tttaacataa tgaaaaaata tggtgatggt
     9241 gttatgtgtg tatatccagc tgaccattat ataaaagatg aaaaagaatt taaatctatt
     9301 ttggaaaaag ctatctatat agcagaaaat aatgataaac tagtaactat tgggataact
     9361 cctacatttc catcaacagg atatggatac ataaatttta atagagaaaa tactatagaa
     9421 gatgttgcat atgaggtagt ggagtttgta gaaaaaccaa actatgaaat tgctaaagaa
     9481 tatgtaaact ctaaaaagta tgtatggaat agtggaatgt tcgtatggaa ggtatctaaa
     9541 atactagaag attttaagag atacttgcca aaagtatatg aaaaattaga agatataagt
     9601 aaatacttag gaacaaaaga agaaatggaa aaaataaaag aaatataccc aactatacaa
     9661 tcaatctcta tagattatgg tattatggaa agaagtaatg atgttatagt agttcctggt
     9721 gattttggat ggaatgatgt tggttcttgg gattctctag gtgcaatata tccaactgat
     9781 gatgaaggta atatcaaaag aggtgaaaat attactatag atactaaaaa ttcaattata
     9841 tattcagatg acaaattaat atcaacaata ggtattagtg atttgatagt agtatctaca
     9901 aatgatgcag ttatggtttg tagaaaagat aaagctcaag atgtcaaaaa aatagtagag
     9961 cagttaaaag aagaagatag acaagaatat atgtaggagg tgttcatttg attcctaaaa
    10021 aaatacatta tgtatggttt ggtggtccaa aaggaaatat agaaaatata tgtataaatt
    10081 cttggaaaga aaagcttcca gaatatgaga tagtagagtg gaatgaaaaa aactttgata
    10141 tagagaaaga aataaaagga aataagtttt tggaagaatg ttataaaaga aaactttggg
    10201 cattcatatc tgattataca agaataaaag tactctatga acaaggtgga gtttatatgg
    10261 atacagatat gcaaatatta aaggatataa ctcctctttt agaaaataat aggctagtct
    10321 gtgggtatga agatgataga gagtatataa atggtgctat aataggtgta gaaaaaggac
    10381 atcctttttt aaaagactta ttggagtatt atgaaaaaga agtacttact tcttcattgt
    10441 ttacaatacc aaagataatg actcatctta tggaaaagaa ttataagaag attgatccaa
    10501 ataattatga agaaggaata cgtgtttacg acaaagaata tttttatcct tttggattta
    10561 aagaagactt tacaccagaa tgtataacag aaaacacttt tgggatacat tggtggggaa
    10621 aaagttgggc taaaaagaga aattatttct tagaaagcaa acacttaaca ggtgtaaata
    10681 agatatggaa gtgttgtaaa atatttgcaa gtaatacttt acgaagctaa aagggagaaa
    10741 gtaaaatgaa gactttaaat gatgatagaa atattatgat tagaaaatat ctttatatat
    10801 ttgttttgtt tatcatatta cttagaaagt atatgatatc atttattgac ccaaatatgg
    10861 atatagggat gattaaatca gcgttatttt attcttcaat aggaatacta atgctacttt
    10921 ttttatttga taagagaaaa agtataacag aaatggtact agttggagtt tgtgtcttac
    10981 tttatttatt aaatagggaa ggagctatat tattaatcgt tttattagct gtatcagcta
    11041 agcaaataga tgataaattt atagttaaaa attatttaat aatatcggca tgttttttaa
    11101 tggtagctat attattattt aatttatttc cttcattaat atttaatcag gaagtccact
    11161 atagatatat agaaaaaata gatatgcttg ttacaagaat ggattttgga cttggtaatc
    11221 caaatagtgt attttatcat atggttacta tatatgcagc atatatattc ttaagatata
    11281 aagattataa taaatgggat agaatacttt tatttggttc tgcatttttt gtttatcaaa
    11341 ccacatatag tagaactgga tttttcacta tattagcagg attaatattt gtagaaataa
    11401 taagatggat agatataaaa aaaataaaag gactaccaat gctcctaaag acattaccgg
    11461 taatacttac tttatttagt gtaattattg gtacagtatt tgataaaagc actttattaa
    11521 atagattatt agcaagtaga cctaagttct ggaatgtata cttggctgaa aaggggaact
    11581 ttctaaaacc ttttggaaat tcatatagtc ctgctataaa agcaactaat ccattagata
    11641 gttcttatgt atatataatt agtgtactag gagtagttgc ttgtatatta tttatgtatt
    11701 tgatgtataa aggaatagaa agctttatag aaaaagataa aaaagcatat ttagtagctt
    11761 tatttatatt tttactatac tcttttgcag aaaatttatt attagaagct tcatttagtt
    11821 ttgcagtagt attactaata aaagaagtga tattaaatga taaaagggaa atagatttgt
    11881 ggaagatgaa aagtaggagg tagtaatgtt aatatcatta ataatgccaa cattaaatag
    11941 atatgatgat atatacttac ttatggacag cttagaaaat caaacttaca aaaactttga
    12001 acttatagta gtagaccaaa atgataatag caaagttaaa gaaatagttg ataaatacat
    12061 tgataagtta gatataaagt atataaaaag ctctaaaaaa ggattgagtt ataacagaaa
    12121 tgtaggcata gacaatgctg tagggcaaat tataggtttc cctgatgatg attgtgtcta
    12181 cgaaaatgat acattagaaa aagttataaa tttctttaat aaaaataaag attataaaat
    12241 atacagttgc aaaactatgg attcaaataa agttgatgca tttaagaaaa tgtatgatgg
    12301 aacttgtgat atcacaagtt ccaacgtttt ggatactata acatccataa ctttttttat
    12361 tgattttgaa ggtaaagact atacaagatt tgatgaaaaa ttgggtgttg gtggagagtt
    12421 tggtgctggt gaagagatag attatgtttt aaacttattg agtttaggtt ttaaaggaaa
    12481 atactttgga aatgatataa tttaccatcc tgcaaaaaaa cattctaaat ctaaagaaaa
    12541 gtatcaaaaa gattataact atggaagagg ctttggagcg ctttgcaaaa aggaaatagt
    12601 ctatagaaaa aattataagt ttgcaaaagt tatggtatca aaacttgtaa gaaatatagg
    12661 aggtcttata ttgagttcca atagagatta tcatagtgcc actataaaag gaagaattaa
    12721 tggatttaga cagtacaagt tataatgagg atggtgttta tggacaaggt taaattttta
    12781 ataaatatgc ttttggcttt ttttatatat ccatttaata agcataagtt taatggtaga
    12841 gatatctggc ttataggtgg acattcaggg gatatataca atgataattc aaagtttttt
    12901 tatgagtata tgctaaaaga acataatgat gtagaaactt attgggtagt aaataaagac
    12961 agtaaagtat ttgataaaat tccaggcaaa aaattaataa gaggaagtgt agaaaactat
    13021 ctgtattatt acaattcaaa agctatagta ttttcacatg caccatcagc agatatagca
    13081 ccatacaatt ttgctgtacc tgttcttaat tattttcata aaaaaacgat aaaagtgttt
    13141 ttaaatcatg ggactataag ttttaaaaaa agaaaaccta tgaacaaaaa gttcaaaaat
    13201 attatagata acctatataa aagctataat atagttacag caagttctga atttgaaaga
    13261 aatgttatgg taaatgattg gggaatgtta gatgactcag tttatataat tggaaatgca
    13321 agatatgaca atttaccaac aaatgaagtt gcacaaactc gagatatatt atatactcct
    13381 acatggagag attggataaa atttagttca ggaaaattta cggatacaga ttattttaaa
    13441 aatataatga attttttaaa tgatgacaaa ttaaataaaa tattagatga gaaagatata
    13501 aatgtaaaaa tttatatgca tcatcttatg catgagttta tagatgatat aaaagaaaat
    13561 ataacaggaa aacgaattgt gtttttagat aaaggggtaa ctctagcaaa tgaaatcaga
    13621 aaatcagctg caaatataac tgattattca agtgtagcca tagacttttt gtatatgaat
    13681 agacctatct tgttttacca attcgatttg gatgaatata tggaaaaagt tgattcatat
    13741 atagatttaa aaagtgaaat gtttgggtct ttggcttata ataatgatga agccgtgaat
    13801 aaacttattg atattattga aaataatttt gaggttatgg ataaccaaaa aaatgaaaga
    13861 aataagtttt tcagatataa tgataataag aactgcaaga ggatatatga ttgtgtatta
    13921 agtaaaatta aataaatatt ttataaatga tgaggaaggt aatatgaaga agaatttagt
    13981 atctataatt actcccatgt ataattctga aaaatttatt gaagcaacca taaaatcagt
    14041 attaaaccaa acttatcaag aatgggaaat gttaattatt gatgattgct caacagataa
    14101 tagtcctaat atagtcaaat cttatatgca acaggatagt agaataaaat gtataaagac
    14161 tgagactaat aagggtgtct ctaatgctag aaatttagca ctaagtaagg caacaggaca
    14221 atttatagct tttttagata gtgatgacca atggaatagt agtaagttag aaaaacaagt
    14281 aaattttatg ttagaaaatg actatgtaat ttcatttact tcatatgaac tgatggatga
    14341 aaatgataaa aaattaaaca aagtaataaa agttccacct aatgtagatt atagaagatt
    14401 attaaaagga aatatcttag gatgtcttac tgttgtgatt gataagtcta aattggattt
    14461 tgaaataaga atgagtggtg taagacatga agattatgtt ttatggttat cgatattgaa
    14521 aaaagggcat attgctcatg gaataaatga agttctagca ttatatagaa aatctagcaa
    14581 ttccctaagt ggtaataaaa taaaagctgc tatgtggact tggaatatat atagaaatat
    14641 agaaaaaata cctttataca aagctatata ctactttatt aattatggaa taaacggaat
    14701 caaaaaaagt tagacagaga ggaagattta attaatgtat aaatataaat ttactgtatt
    14761 tactccgacg tataacagag ctcatttact tgaaaatttg tacaatgatt taaaagctca
    14821 aacttatgat ttcaatgatt ttgaatggtt gattgttgat gatggaagta gcgacagtac
    14881 taaagagtta gttgaaaaat ttatatcaga aaataaatta aatataagat atatatacaa
    14941 agaaaatgga ggaaaacata ctgcaataaa tctaggtgta aaaaatgcag atggagagct
    15001 atttttcatt gtagatagtg atgatggttt aattaaagat tctcttgaaa ttgctaatga
    15061 tgagtgggat tctttagaga ataaagaagg atttagtgga gttgttggac tttgtgtata
    15121 tccaagtgga gatttgatag ggacagaaat gcctgaagat aagaaaatat gtcactttgc
    15181 agacctatat tttaagtatg gcgttaaggg agataaaagc atagtatttg taactgatga
    15241 gcttataaaa tttccttttc cagaaagaaa agaagtgaga tttttaccag aaagtgtagt
    15301 atggaatgaa atgtctaaat actataaagt tatatgtgta aataaaccta tgataataag
    15361 agattatttg gatgatggtt tgactaagaa tatattaagt aaaaatgcat taagaggtag
    15421 agcattagag tttttatatt taataaatca aaatacatat ccattaagta gatatccata
    15481 tatgtggata aaaaattaca taaatttagc cagatactca ttgttgtctg atagtcatta
    15541 ttttggtgaa ttaagaaaag tatctgataa attgttgtat ttagcattat tcccattagg
    15601 atattacaaa tatattggac aaagaaaatt agtatctaag taaacttgtt ggtatagtac
    15661 tgctagttgc tatataagga gagtaaaaat atgaatgagc ctttagtatc gataattact
    15721 cctgtttata attcagaaga gtttttatca gagacgataa agtcaatcca aaatcaaact
    15781 tataaaaatt ggcagctttt attagtggat gattgttcta aggacaattc aagtgaaatt
    15841 ataaaaagtt ttcgaaaaga agatgctaga ataaaatata taaagcttga aaaaaattct
    15901 ggagctgcag tatctagaaa tgttggtatt aagaatgcgg aaggtcgatt tattgcattt
    15961 gtagatagtg atgatttatg ggatagtaga aaattagaga ttcagataga atatatgtta
    16021 aaagaaaatg taggtttttc ttttacatct tatagatata tgagacaaga tgggtcaaaa
    16081 acgaataagg ttgcaagagc tcctaagaaa attgactatg aaggattact tagaaatact
    16141 ataataggat gttctacagt ggtcattgat aaggagattg taggagagtt cagcatgcct
    16201 ttggtaagaa gaggacaaga tactgcaacc tggttacagt tacttaaaaa ggaaaaatat
    16261 gcatatggga ttcaagaaga tttagtgaat tacagattag ttggaaattc tatatcaagt
    16321 aacaagataa aagcattaaa acgaacttgg aatacttata gaaatgttga aaatttaagt
    16381 ttaccaaaaa gtctatatgt attttgtttc tatgttttta atgccattaa aaaaagagta
    16441 taagttttat atacttggag gtaaaaaaat gaaaatagct atagctggga cgggatatgt
    16501 aggtttggtt acaggtgttt gtctagcaga ggtaggcaat gaaaatgtag tctgtgttga
    16561 tgtagatgaa caaaaagtta aatcaatgaa acttggaata gcacctatat atgaagaagg
    16621 cctagaagaa cttatggtta aaaattacaa cagaggaaga atagattaca ctacagacta
    16681 taaaaaagct tatgaagatg tagatgtaat attaatagct gtaggtacac ctgagagaag
    16741 tgatggttca gccaacctag attatataaa ggtagttgca aagcaaatag ctgaaactgt
    16801 gacaaaagat actctagtag taataaaatc tacagttcca attggtacaa atgaagagat
    16861 agaagattat ataaatagaa acctaaaaaa tgatgtaaaa gtagaacttg catctaatcc
    16921 agagttttta tcacaaggaa cagcagtaaa agatactcta catgcttcaa gaatcgttat
    16981 aggtgtagaa agtgaatggg ctagagaaat attagaggaa atctataagc cttttaatca
    17041 acctataata gttacaaata gaaatagtgc tgagatgata aaatacgcat caaataactt
    17101 cttagctctt aaaatatcat ttatgaatga tattgcaaat ctttgtgaaa tagcaggagc
    17161 taacatagaa gatgtagctt taggaatgag ttatgatgat agaataggaa gtaagttttt
    17221 aaaggcggga ataggatatg gtggttcttg ctttccaaaa gacacaaaag ctcttcactg
    17281 gttatctgaa cataatggtt atgaattaaa aacagtaaaa gctgcaatag aagttaatga
    17341 aagtcaaaaa cttaaattag ttagaaaggc atctaagtta gtagagagct ttcaagatat
    17401 gaaggttgct gttttaggtt tgacttttaa gccagacact gatgatataa gagaagcacc
    17461 atctatacca aatataaaat atttgctcga aagaggggca agagttactg tatacgaccc
    17521 aattggtgta aataatacta agaaaatatt tggtgactct atactttatg ctgattctgt
    17581 agatgatgcg atagaagatg cagagatatg ttttataatg acagaatgga aagatgtatt
    17641 aaattatgat atacataagt ataaggaaaa gatgaaaaat cctttagtcc ttgatggaag
    17701 aaattgttat aaaattaaag atatggaaga gttaggaatt gagtattatt cgataggaag
    17761 gtaaatatta tgaagactgt ttgttattta gctgatgggt caaatcctca tactttaaaa
    17821 tggtgtagtt tttttaaaaa taaaggatat gacattcatg taatctcttt aaatggaggt
    17881 cacatggaag gaataaaaat ttataatttt ggttctaatg tagaagaact aaaaaatgaa
    17941 aatatttcta aaaaaacggg atacttaggt tcaataaagc agataaagaa gctagtaaat
    18001 gaaataaagc cagatatatt gcatgcacag tatgcaagta gttatggatt tataggaagt
    18061 ctacttgggt atcatccata tattatttct gtttggggta ctgatattta tgatttccct
    18121 aataatggtt ttatacaaaa acaaattata aagcataact ttagaaaagc agactatatc
    18181 ttttcaaata gtagagatat ggctaaagag gctaataaat atacagctaa acatgtagat
    18241 gtaacctttt ttggagtaga tatggatagg tttaagccta tggaagtaga aaaagaagac
    18301 gcttttgtaa taggaattat aaagagttta gaaaaaaaat atggaataga atatttaata
    18361 caagcattta agatgcttaa agatgaatat aaagataaga aaataattct taaaataggt
    18421 ggctctggaa gtcagatgga caacctaata aatttagcta aagaattggg tatagaaaat
    18481 gatgttcagt ttttaggaag aatatctcca gaaaatgtaa gcaagacttt taattctttt
    18541 gatgtaactg tatttccttc tttaagagag ggatttggag tggcagctat agaatcagaa
    18601 gcatgtgaag tacctgttat agttacaaat gtaggaggac atccagaatc tgtatgggaa
    18661 aatgaaactg gcttaattgt agaaccaaaa caaccagaag aaataaaaaa tgctataata
    18721 aaactaatgg aaaatgatga acttagactt aatatgggta agaaagggcg tcaatttgtt
    18781 agagaaaatt atgaggttaa tttaaacttt aatgatatag aaaaaattta tgattctata
    18841 tttgataaat ataaaaaata aaatgaaaaa atacagcaaa agatagatgt tgttggctat
    18901 gcttaggtgc gtagcttttg ctgtttaaag gagggcttct agttatgcga gaaagtatag
    18961 atttagagaa agaaagcttt tttaataggc tttttaaaaa atataaattt agacatttaa
    19021 cactattaat tctagatatt tgtagcgtac tagtagcgtt tcgaatgtct ttaagcttga
    19081 caggtaattt aaatgcattt accactaatg atataataat ttcagtgtgt atatatatag
    19141 tacttcatat agtatcattt agattgttta agtgctacaa cacattatgg agatatgcag
    19201 gagaagaaga aattatatct atttttgtgg caactttagc ttatttaatt ccgatttata
    19261 taataaataa attattaggg tttgattatc ctataatgtt ttatgtatta aatacaatat
    19321 ttattataat gtttacaagt ggcgcaagaa tagcctatag agctataaga atagtcatga
    19381 acaagacata ttctagagga aaggtttcaa atatccttat aattggtgca ggagatgcag
    19441 gagaaatggt tatacaggaa ctaaaaagaa atccagaact taagaaagta gcagtggcta
    19501 taattgatga tgataaaaat aaaattggaa gaataatcca taatgtgaaa attgtaggaa
    19561 caacttcaga tataaaagat gtggtagaaa agcacaatgt agatgagata atcttttcaa
    19621 tagcaaacat agagaaacgt agaaaaaaag agattattga tgtatgtaaa aacactaact
    19681 gtaaaataaa gacaatacca ggaatttacg aaattataga tggaaaagtt gatataaaac
    19741 agattagaga agtagagata gaagacttgt tgggtagaga gcctataaaa acaaatttaa
    19801 gagagataag taattacatt gagggaaaag taatcctaat aactggagga ggaggctcaa
    19861 ttggttcaga gctttgtaga cagattgcag gatttaatcc taaagagctt attatagttg
    19921 ataattatga aaataatgct tactcaatac aacaagaatt acttagaaaa tataaaaata
    19981 aattagattt aaagacagta atagcatcaa taagagaaga aaaaagaatg gatgaaatat
    20041 ttaataaata taaaccagaa gtggtatttc atgcagcagc acataaacat gttcctctta
    20101 tggaatcgag ccctggcgaa gctataaaga ataatatatt tggaacttta aacatagctg
    20161 gcttgtcttc aaaatataga gctaagaaat ttgtccttat atctacagat aaggctgtaa
    20221 accctactaa cataatggga gctactaaaa gagctgctga aatgattata cagactatga
    20281 atgctgaaag ccagactgaa tttgttgctg ttagatttgg aaatgtgctt ggtagtaatg
    20341 gtagtgtaat tccacttttt aagaagcaaa ttgaagatgg cggaccagtg acagttactc
    20401 atccagatat tattagatat tttatgacaa taccagaagc agtaggctta gtcatacaag
    20461 ctggggctat ggctaaaggt ggagaaatat ttgtacttga tatgggagaa cctgtaagaa
    20521 tattggattt agcaaaaaac ttaattaaat tcagtggatt tgagccagat gttgatataa
    20581 aaattgaatt ttctggtctt agaccaggag aaaagttata tgaagaacta cttatgtcag
    20641 aagaaggtct gttggataca gagcataaaa agatttttat tggtagacca atagatgttg
    20701 atagagaaaa aataacaaaa tacttaaaac ttttaagaga aattactaac aatgaagagg
    20761 ttgaaaaaat agatggtatt atgagggaat tagtgccaac ttatattaaa ccagaagatg
    20821 ctaatatcaa agaaattgca acaactagag aaaagtagaa tatatttcta atttgtaacc
    20881 agggggagca aaaagaaaca aatatatact aaaaagaaac aaaagattac aaaaagatta
    20941 caaaaagatt acaaaaaagg ttgcaattta ttttgatgta agatagaatg ttaattagga
    21001 attggaaacg aaggaacaaa atgttaaatt ttcgaaagga ttaggtattt gagattatga
    21061 tgaatattaa aaataaaaaa catatattga aaaaatttat agctatggtg ttaatagcag
    21121 gagttgtcac agtagaagca ggagcaataa cggctagtgc agcagagcca actaattcac
    21181 ctatgagtgc aacagtagac cagtgtgatt ttttaaatgt aagaagtgga gctagtgcaa
    21241 atgatgcagt agtaggaaag ataaacacag gagacaaagt tgaagtttta gaattacatt
    21301 caaatggatg gattaaaata aaaagtgttg acaatgttac aggatgggta aatggagatt
    21361 atttaactat tcaaggtgga aatgtagatg caaaggtaca aaatgtttta aacttagcat
    21421 ttaagcaaca aggtaagcca tatagatggg gagcaactgg accaaatgct tttgattgtt
    21481 ctgggttcac ttcttatgtc tataaaaatg gagcaggagt taatttacca agagtatcaa
    21541 gatcacaagc aacagtagga aagaaagtat caagagcaga attaaaacca ggagatttag
    21601 ttttctttgg ttcaggtggt tcaataaatc acgttggatt gtatgtagga gattctaaat
    21661 tcatacattc accacaaaca ggagatgtag taaaagttac tagcatggct ccaggaacta
    21721 attatgcaag aagacttata actgctacac gtgttttaca ataataatag atattgattt
    21781 acataggatt gaatattaac attaaatata taattacatt taatgtttta catagagatt
    21841 taagagttta gtaatattaa aatagtgatg aacgttataa aaacagcaaa gaagataaat
    21901 ttctttgctg ttttttttat aataaattat ttcatattat aatcaaatat caaatataaa
    21961 ttttaactat attattcaat atttgacaga taatatgaat tttagatata cttaataaga
    22021 agtatattgt agaaaaaata aatttaaaaa attaaggggg atgaaatgaa aaaaataagt
    22081 atattagtat tatcattgat aatgacactt actatgtgta gtgtaagttc atttgctgat
    22141 tcatcaaatg ataaagaaat gagagctgct tggatatcta cagtttataa tttagactgg
    22201 cctaagacta agaataatga agccaagcaa aaaaaggaat acacagattt attggacaaa
    22261 ttaaaaagtg taggaataaa tacagcagtg gttcaagtaa gaccaaaatc agatgcttta
    22321 tataaatcaa atataaaccc atggtctgaa tatcttaccg gtacacaagg taaagaccct
    22381 ggatatgacc cattaccatt tttaatagaa gaagcacata aaagaggaat ggaatttcat
    22441 gcatggttta atccatatag aataactatg gcagatgaga gcatagataa attgccagca
    22501 aaccatccag caaagaaaaa tccaagctgg gtagttaaac atggaaataa gtattactac
    22561 gacccaggtt taccagaagt tagaaagtac atagtggata gtatagcaga agtagttcaa
    22621 aattacgata tagatggagt tcattttgat gattacttct atccaggagt aagtttcaat
    22681 gatactgcaa cataccaaaa atatggaaaa ggacaaaata aggatgattg gagaagagaa
    22741 aatgtaaata ctctacttag agatgtaaaa gcgtctataa agagtataaa gcctaatgtt
    22801 gtatttggtg taagcccagc aggtatatgg agaaataaga gtagtgaccc aacaggttca
    22861 gatacatcag gtaatgaaag ttatgtaggt acatatgcag atacaagagc atggataaaa
    22921 caaggattga ttgactatgt agttccacaa ctttactggc caattggtct taaagcagca
    22981 gattattcta aattagtggc atggtgggct aatgaagtaa aaggaacaaa tgtagactta
    23041 tacataggac aaggtatata taagcaaggt caatcgagtt atggtggaca aaatatagca
    23101 aaagaaatag tacaacaagt aacattaaat agaaagtata gtgaaataaa aggtagtatg
    23161 tatttctcag ctaaagatat agcaaatagt acttctatac aaaaggattt aaagagttta
    23221 tattctagta gtgaagaacc agttactcca ccatctaatg ttaaagtgga gaaattaaga
    23281 ggcgacgaaa gatatgacac agcagtagca ataagtaaaa aaggttgggc tacaaattca
    23341 gatacagtag tattagtaaa tgggtactct atagttgatg gtataacatc aacaccactt
    23401 gctacatcaa atgatgctcc tatactattg gtaaataagg ataatatacc tacatccact
    23461 aagaatgagt taaaaagact taatccaagt aaagttattt taattggagg aaataactct
    23521 attggagata aagttgagag tgagataaaa gatacattgt caaatgtatc aataaataga
    23581 gttggtggtt cagatagata tagtacatct ttaatgatag caaaagaatt agtaaaaact
    23641 aatccagtag aaaaactata tataacaagt ggtacaggtg aagcagattc tctttctata
    23701 gcttcaaaag caggagaaga aaagcaacca atagtattag tatcaaaaga taatgtaagt
    23761 gatgaagttt acaattggat atcagattta aaagtaaaag atgcttattt tataggtggt
    23821 aatctaagta tatctgattc agtaataaat aagctggata aggtcattac aaatgatgta
    23881 tctaaaaata gaatagctgg agaaaataga gaagaaacaa atggaaaagt aatacaaaaa
    23941 ttctatccaa atgctgaata ttcatcaatg tttgtaagta aaagtaatca attggtagat
    24001 gcattaacat caggttcttt agcggctaaa ttaaaatcac cagtagttat gcttggaaat
    24061 agtgtaacat ctgctcagaa aactgcttta gagcctaaga aaactactct agtatacgaa
    24121 gctggagatg gtataaatca aaacacatta aacactttct taaacttagt taagtaataa
    24181 aaaagttaat ttaaaataca gagtatttta caaatgtaga ttaaaatgaa aattatcatg
    24241 ataaattaga aaataaaaaa gaactatatg cattttgtaa tcaagtgtac acagcaactt
    24301 gaaaataatt atgcgatata gttctttttt agtaattaat cttatacttt ttaataaact
    24361 ttatgttgaa ttttttattg tttaaactct atgtcattaa atataaaatc gtgtaatatg
    24421 tctaaagtat ctgggtcaaa tctaagaacc catccagctt ttccatatat accacctgta
    24481 ctatgaattt catcatcaat aggaaattca aattgcttaa tatttaagtc acccattttt
    24541 aaaacttgag ctcctaaaga aagtattgca ttaggcttca tgttagtttt aacatatgga
    24601 agaactgcat ctaataaacc tggatattta gtaacaggta aagtttccgt tttcttgatt
    24661 atagcttgta taatctgtct ctgtctgcca tctctagcaa aggctgtatc gttgtgtctg
    24721 atacgtgcaa aagatagtgc ctgatatcca tttagagtct gctttccaga gtttctaatg
    24781 tattgtatgc tacctttatc atcactttta ctccatttat aagtttcagg aataaattta
    24841 tttagttctc gtatttctcc tttttcaaca gtaacctcaa cgccaccaat agcatcaatt
    24901 atatacataa atgattcgaa attaacacaa gcataatttt gaatatcaat attaaaattc
    24961 tcttcaattg tttgtattaa taaatcagct tgtccataag cataagcatg agtaagtttt
    25021 tgtttaccat gaccagggat gtctacatag ctatcacgag ctaatgatgt aagtttgata
    25081 tcattgtgtt tgttatcaat agtaagtatc atcatagcat ctgaccttga agaatcttca
    25141 tttggtctag catctgttcc cataagtagt atgttaatta tcccatcttc atttttatgg
    25201 tcattgttat ccaataaatc actacttatg ctcgagtcat gaatagcaga taatttataa
    25261 taaaaatatc catagacact tatagggaat attactacca agaaagccag tagtataaca
    25321 aatttcttta attttgacaa aagtacccct tctttcttct ttttcctgta tattttttaa
    25381 tagcatacac ttaattatat taaagtattg agatatattc aatacttgtt tcagttacaa
    25441 aaaaaagaca atgatataat ttataagaaa atttatcgac ctatatatat agttaaaagt
    25501 atatataata tattttttaa aagtaattta tattttaaaa taaaaacaca ccccataaat
    25561 acaaagtaat ttgcatttaa gaggtatgta tttatcatca aattattggt ttgttttgga
    25621 atttctaatc ttcaaccata atatctttaa atatgaaatc atgcattgca tctagttcat
    25681 attcctcaaa aggtattaca tagcctgcat tacctatttt tctaccattt tcagtagaaa
    25741 gaggaaactc catagatttc aaatttaaat ttcctatact taaaagttct tttcctaaag
    25801 atattatttc attaggattc atattagttt ttacatacgg aagtattgtg ttaactagat
    25861 taggatattt agtgactggt aaatccttaa caccatttat taaactctgt ataacacttc
    25921 tttgtcttct atctctttca tctgtactat cattttttct tatacgagaa aatgcaagtg
    25981 tctgatatcc atttaatttt tgctcaccag aatttcttat atatttaaga gaacctttat
    26041 tgtcattttt attccattta taagtttcag gaataaattt atttaattct cttatttcac
    26101 tcttatcaac agtaacagta actccaccta aagcatcaac tatatccata aaagagaaaa
    26161 agtcaactac ggcgtagttt tgtatatcaa gttggaagtt ttcttctata gtttctatta
    26221 ataagttagc ttgtccatat acgtaggcat gtgttaattt ttgttctcca tgtcctggta
    26281 tattaacata agtatctcta tttaatgaag ttagcttaag acttttattt ttattatcaa
    26341 tagtaagtat catcatggca tcagaacgag aaacttcttc tcctggtctt gcatctgtac
    26401 ctaacaataa tatgtttgtg atattttctt cgtttttata gtcattacta ctaagcgcct
    26461 ctgtatttat actatcatct tgtatagttt ttaatttagt aaaaacatat ccatatatac
    26521 taacaggaaa tgctataact aaaagggcta gaaatataac gaactttttg agtcctgaca
    26581 ataatatccc ctactttcta aattttttaa ttacacatag ttaattatat taaaaatttg
    26641 ttacataagc aataaaaaac acaataaatt agcataagtt tgtcataata taattatttt
    26701 tatagcattt tatagaagaa agtgtgtagt tattattgat tttattggaa tataattgta
    26761 tgaagactta aatttttata aactaatgtg tatattgaaa aaaatatttt cctgaatgta
    26821 gttatagcat cttatgaaag gtgcaaattg taatttaaaa tttttagtgt ataataagaa
    26881 ttgaaaattc aaaccgataa aaattaataa aaaataatta taaattaata atttggggga
    26941 ggaaatgtat gataaaatta atagcaacag atttagatgg aactttgtta gatgaaaaga
    27001 gcgaaataaa tccagagttt tataaagtat ttaaaaaatt aagagagaga gggataatgt
    27061 tttctgctgc tagtggcagg cagtatcaaa atctgataaa aaaatttgaa gatataaaag
    27121 atgatatgat gtttatatca gaaaatggaa cactagtagt atataaagga aaagaaatat
    27181 tatcaaatcc tttaaataaa gaattagtaa atgaaatcat agagacaaca agaagtataa
    27241 aagggaaaaa aattgtaatg agtgggaaaa aatacgctta tatagaaagc aaagatgaag
    27301 cgtttataca agaggttagt acttattatg caaagtttaa aattgtagaa gatttgacga
    27361 aagtagaggg agacatattg aagatagctg tgtttgactt caaaggtgca gaacataata
    27421 ataatatcta ctttgaaaag ttttctgata gagctcaagt atgtatttct ggagtggagt
    27481 ggcttgattt aactgcaaaa ggtgcaaata aagggagtgc cataaaaaaa gtacaaaaaa
    27541 tgttagatat aaaatatgaa gaaactatgg tatttggtga ccagttaaat gatgtggaaa
    27601 tgatgaaaag tgcctatcat agttatgcca tggaaaatgc aaatgaacat ttgaaacaaa
    27661 ttgctagatt tagagcaaaa agaaatactg aaaatggcgt tgtagataag ataaaagagg
    27721 ttataaaaat tggatagtca tatgtataga tgaaaaagat tattttgaag aaatcaaaaa
    27781 aatatcaatg taattttgat aaataagttt atttatgcga aaattacatt gatatttaat
    27841 aaacatcaat aatagatatt tattttttta tgatattcag tattttgttt tctatttgag
    27901 ttgcattttc agcagaaatt aaataaacat acctacctgc cttctcaatt atagtattat
    27961 ttgctatttc agaattgatt ttttctccat gtccttctcc aaaaggtgct tgtatcatat
    28021 cttttttata attttctaga gttttataga ttttatctac ctcgtcagtt tttataacga
    28081 taatatcttc tagagataat ttttcagaag aattaattac aaaaccttga attatgtctt
    28141 cctctacatc attaaaatag ttaaaatcct ttgcatcaaa gatttttgat tcctttataa
    28201 gtaaatcaga agattttaat gcttttttta tttttgatgt agaaacgttt ttgtaatcac
    28261 cacttgaaga acaactaacc attcctaatg ataagattat cgaaaaaaac aatattaaaa
    28321 tttttttcat gataaacctc caagtttttt aaactctgtc ttaaattagt ttactacata
    28381 attatataaa aataaatgta tttaatgtta cagttttgat taataatgta atattttgat
    28441 aataatacct attgagaggt gattagaaat gagaatacca aaacatgtag gaattatacc
    28501 ggatggaaat agaaggtggg caagtcaaaa tggtatgaca aaagataaag gatatgaaag
    28561 tggaatagac cctggactag agtcttatag attatgtaga gatttaggct gtgaagaggt
    28621 gactttttat ggatttacaa ctgataatac aagacgtcca tcagtgcaga caaaagcttt
    28681 tacaaaagca tgtattgatg ctgcaaatat attaatagat gaaggtgctg ctttattagt
    28741 tgtaggaaat acaaaatcgc caatgtttcc agaagaatta ttaccatata ctaatagaat
    28801 taaagccaaa gaaggtcaaa taaaagcaaa tcttctggta aattatggtt ggtattggga
    28861 cttaaataat atatcaggag tagaaaatgt aaataaaaat aatatacagg attacataca
    28921 gtcatttgat gtatccagac tagacttgat aatcagatgg ggtggaagaa ggagattaag
    28981 tggtttcctt cctgtacaat caatttatgc agatttttat gttatagata attattggcc
    29041 agattttaag agtgacgata taaaggacgc atttagttgg tatgatacac aagatgtcac
    29101 tttaggtgga taattaaaaa tattctgatt taaggttgta gagtgtttta aattattgaa
    29161 aaaaaaactt cttgatggta aaataacact aataatatgt aaaagtgtaa aaatttatac
    29221 caagaagggt gaagaaatgt caaaaggcaa taataacaat aattcaagaa acaaatctaa
    29281 aaagaccagt catctgaata gaaaaaagcg aaaattaaat aagaaaaagt tagcagtgtt
    29341 gatttgcttt acagttttat ttttatttat tgcatttaag gctactcaag gtgtagtagc
    29401 tttggtaaaa agtatggata aaagtaataa aactagtcaa caacaaaatg ttaactcaga
    29461 acaatttgac cttggtaatg aagaggaaaa taaaaagaaa aaatatactg tatttataga
    29521 cccaggtcat ggaggcaatg ataaaggaac tgaaagcaag accagtaata gatatgaaaa
    29581 agacctaaat ctacaaattg caaaaaaatt agcaaataaa ttatctaaac aaaaggatat
    29641 tcaagtagta gtatccagaa ctgatgatac atatatatca ttaaaggata gagctatctt
    29701 agcaaataat agttctgctg atgtgcttgt ttctattcat ttaaatgcag agaaaaatgg
    29761 taatacagca acaggtatag aaacttggta taggaataaa gctacagatg gttcaaaaga
    29821 acttgcacag acagtacaaa gtactatagt atcctatgtt aaagttagag ataggggaat
    29881 agttgaaaat aactttgaag tacttagaga atcaaatatg cctgctatat taatagagtg
    29941 tggtttttta acaacacctt cagaggaaca aaaaataata aatgaaaaat atcaagacca
    30001 acttgcggaa ggaattgttc aaggtgtact ttcataccta gattctaaag ggaataagta
    30061 aattaacttt actttaatgt gaggataaat ttaaaagttt aactgtgtaa attgtattaa
    30121 ctttatttcg taatgttgga aatattgttt gaatttagaa actatgaaat tagaaattat
    30181 tttatctata tctaatttca tagttttttt tgcaaaaaat ttatagttat aaaattttaa
    30241 aaaataaatt tagtataatg taatgtagtt atttatatgt gattttaact tgcattgatt
    30301 tgctatcaat tgtgctttat aattagtata tataaagttt aactgtaaga taatttaaaa
    30361 aataggggaa tttatatgaa taaaaatagc atgacaagaa tttacagttt aaataaaata
    30421 atttttatta taattggaat t
//
LOCUS       DACULQ010000027        30068 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.027, whole genome shotgun sequence.
ACCESSION   DACULQ010000027 DACULQ010000000
VERSION     DACULQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.027"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(189..767)
                     /locus_tag="JA154_05115"
     CDS             complement(189..767)
                     /locus_tag="JA154_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAT7995239.1"
                     /translation="MISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFLKIDLSDIPA
                     IFGGMSLGPFVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMILCYSYKRSGD
                     LKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINPQVHDLFTFV
                     IWMIAPFNVLKAVILSLVTLPLYKKMGNVIKK"
     regulatory      complement(918..1035)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1095..2261)
                     /locus_tag="JA154_05120"
     CDS             complement(1095..2261)
                     /locus_tag="JA154_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7995240.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(2409..4550)
                     /locus_tag="JA154_05125"
     CDS             complement(2409..4550)
                     /locus_tag="JA154_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAT7995241.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLEKLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMPPHRVL
                     AINRGEKENFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DNEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKTIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNICEIDEKIKEIGLRELSEKLEVGQVTLKDIITEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(4694..5801)
                     /locus_tag="JA154_05130"
     CDS             complement(join(4694..5725,5727..5801))
                     /locus_tag="JA154_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621577.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; catalyzes the release of
                     newly synthesized polypeptide chains at the stop codons
                     UAA and UGA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAT7995242.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNKQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(5816..8491)
                     /locus_tag="JA154_05135"
     CDS             complement(5816..8491)
                     /locus_tag="JA154_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAT7995243.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(8623..9174)
                     /locus_tag="JA154_05140"
     CDS             complement(8623..9174)
                     /locus_tag="JA154_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal subunit interface protein"
                     /protein_id="HAT7995244.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(9727..10401)
                     /locus_tag="JA154_05145"
     CDS             complement(9727..10401)
                     /locus_tag="JA154_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAT7995245.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(10404..11456)
                     /locus_tag="JA154_05150"
     CDS             complement(10404..11456)
                     /locus_tag="JA154_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HAT7995246.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(11505..12464)
                     /locus_tag="JA154_05155"
     CDS             complement(11505..12464)
                     /locus_tag="JA154_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HAT7995247.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(12560..13078)
                     /locus_tag="JA154_05160"
     CDS             complement(12560..13078)
                     /locus_tag="JA154_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline reductase
                     family selenoprotein B"
                     /protein_id="HAT7995248.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGIMMEQFLNE
                     SKVIATLFCGDEFFVNNEEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            complement(13089..14423)
                     /locus_tag="JA154_05165"
     CDS             complement(13089..14423)
                     /locus_tag="JA154_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAT7995249.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGSDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQVVLLILGIFMYIPFIKMLDKQYLREENATVEEEDDDISFDDLVL"
     gene            complement(14462..14788)
                     /locus_tag="JA154_05170"
     CDS             complement(14462..14788)
                     /locus_tag="JA154_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT7995250.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(14812..15120)
                     /gene="celC"
                     /locus_tag="JA154_05175"
     CDS             complement(14812..15120)
                     /gene="celC"
                     /locus_tag="JA154_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086604.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAT7995251.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(15261..17173)
                     /locus_tag="JA154_05180"
                     /pseudo
     CDS             complement(15261..17173)
                     /locus_tag="JA154_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
     gene            complement(17505..18287)
                     /locus_tag="JA154_05185"
     CDS             complement(17505..18287)
                     /locus_tag="JA154_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAT7995252.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKIVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKDNVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(18290..20509)
                     /locus_tag="JA154_05190"
     CDS             complement(18290..20509)
                     /locus_tag="JA154_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAT7995253.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIANKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHVEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(20773..21870)
                     /locus_tag="JA154_05195"
     CDS             complement(20773..21870)
                     /locus_tag="JA154_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995254.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMTIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(22185..23378)
                     /locus_tag="JA154_05200"
     CDS             complement(22185..23378)
                     /locus_tag="JA154_05200"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086599.1"
                     /note="catalyzes the formation of S-adenosylmethionine
                     from methionine and ATP; methionine adenosyltransferase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAT7995255.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEDTKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(23514..23617)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            23788..24315
                     /locus_tag="JA154_05205"
     CDS             23788..24315
                     /locus_tag="JA154_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HAT7995256.1"
                     /translation="MKGVFLLYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDR
                     AIGDTLGPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDAC
                     LGSQSNIGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFI
                     LDLAETIALALLVST"
     gene            complement(24426..24854)
                     /locus_tag="JA154_05210"
     CDS             complement(24426..24854)
                     /locus_tag="JA154_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HAT7995257.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRRNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(24870..25889)
                     /locus_tag="JA154_05215"
     CDS             complement(24870..25889)
                     /locus_tag="JA154_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086596.1"
                     /note="functions in MreBCD complex in some organisms;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAT7995258.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDNRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(26086..26346)
                     /locus_tag="JA154_05220"
     CDS             complement(26086..26346)
                     /locus_tag="JA154_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein D"
                     /protein_id="HAT7995259.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(26444..27112)
                     /locus_tag="JA154_05225"
     CDS             complement(26444..27112)
                     /locus_tag="JA154_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HAT7995260.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(27178..28242)
                     /locus_tag="JA154_05230"
     CDS             complement(27178..28242)
                     /locus_tag="JA154_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HAT7995261.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKAESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(28329..29600)
                     /locus_tag="JA154_05235"
     CDS             complement(28329..29600)
                     /locus_tag="JA154_05235"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086592.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAT7995262.1"
                     /translation="MRRSKNMAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGK
                     STLNGVPNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFL
                     VMGPLLARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGN
                     KLYLDFPSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNT
                     IKIKGVKELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAG
                     CEITEMDNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFE
                     NRFMHVAEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGET
                     QIGEIYHIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(29603..>30068)
                     /locus_tag="JA154_05240"
     CDS             complement(29603..>30068)
                     /locus_tag="JA154_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995263.1"
                     /translation="DKIQLCATIKGSDKEISIKGINKNNKESYIVVDILDNKVYKHKE
                     VIYSNVENVLNKYSSQVDVSACIVGEYTKRLQNNKYDDILQKILYNMSAKQIDEVKDV
                     NFLSITAYSKVLNDYELEYLGEKINLNIGIKYNEDDGRTLIYIATPIIKLDY"
BASE COUNT         8935 a         5669 c         3192 g        12272 t
ORIGIN      
        1 tagttaatcg ttttaattct tcctttattt taaaatttaa atttgtctaa atatttctta
       61 ttttatatac attttttgat aaattaataa agccataata tatttaatat atcaatcaca
      121 atataggact gaagttataa ataaattatg gctattcaat acatttaatt ttatattttc
      181 ttgtctaatt atttctttat tacatttccc atttttttat aaagtggcaa tgttactaaa
      241 gatagtataa cagccttcag cacattaaat ggagctatca tccaaattac aaaagtgaat
      301 aaatcatgaa cttgaggatt tatagctgaa cctaaaccta ttatagcatc tagtcccata
      361 agttgaccat aaagtggcaa cattacaaaa taatttacta atgaaccaat tatagtcata
      421 gatattgttc ctaatacaaa accaatcaat acacctttta aatctccact tctcttatat
      481 gaataacaaa gtatcattac ataagaacca ccaattaata tatttgcaaa ttctcctatt
      541 cctccagttg ttgttgctgt tattgcttgt aacacatttt ttactacaac tattccaaat
      601 ccaacaaatg gtcctagaga cattcctccg aatattgctg gaatatctga taagtcaatt
      661 tttaagaaat ctggaaatat acctggtatt ggtgcttgaa taaacattaa taaataagat
      721 attgcagata aaattgacat tactactaaa gttcttgttg aaatcatatt gcctcttttg
      781 gctgtgtttt gcataattat ttccccctaa tcaatatgtt tatatttttt aggaaaaaca
      841 aaaaactcga agaccctatc ttcgagtttt tatggatata gtttaaacaa actaaaataa
      901 acttatttat cttccataaa tatcttctct catccagact ttactgtcgg ctttggaatc
      961 tcaccaaatc aaccacttaa gtagttcgcg ggctataccg ccggtaggga atttcgccct
     1021 gccccgaaga tcttctttga ttttttaatt ttccttacac tcttatatta gttaattttt
     1081 taataattgt caagttattt tttctcatac actactttgc catttattat agtgtaaagt
     1141 acattacttt gaatctctag tggagaatta tcccaaatta ctaggtctgc atctttacca
     1201 acttctatag agcctactct atcttctatt cctaaagtct tagctggatt tatcgtaatt
     1261 gattctattg ctttttcttc tttcatacca tgtttgactg caatacctgc acatattgga
     1321 agatattgaa ctggtattac tggatggtct gtcattatac atacatcaag acctgcattt
     1381 gaaagtattc ctgctgtatt aaatgtcaaa tttctaagct caaatttaga cctttctgac
     1441 aaagatggcc ctactattac aggaaaccct tcttccacta attcatctac aataagatga
     1501 ccttctgtac aatggtctaa tgtaagcttt aaattaaatt cttttgcaat tcttattgct
     1561 gtaaacatat catctgctct atgagcatgt gccttaaaag gaatttctct tcttaaaact
     1621 ggtattaaac tttccatttt tatatcatat tcaggttttt cacagtcttc atcatcatga
     1681 ctctcataca tatcaatatc ctctaaatat tcttctgctt tttttaggtt ttctctaagt
     1741 aaagcagcta tagccattct agtttgagga gatttatcat cctgaccata acagctcttt
     1801 ggattttctc caaaagcgat tttagatgca actggatttt ttattaccat tttatcaatt
     1861 ctttttccac atgtctttat agctatacat tgaccaccca taacatttgc acttccaggg
     1921 gttgtacaaa cagaagttat tccaccttca aaagcttctt taaaagttct atccattgga
     1981 ttaattccat ctatgggatt taattgtggt gttattgggt cagtctcttc atttccatct
     2041 gctccttcaa accctatacc atcttcccac aaacctaaat gagtatgagc atctataaat
     2101 ccaggaaaca caagtttatt actagcatct attacctcta catctaaagg agctatcaaa
     2161 tcttctccaa tttctattat ttttccttca tctataagta tatctccatg aattatccca
     2221 tttgttatgg tatttatttt accatttttt ataaaaatca ttcattttcc tcctgatttt
     2281 caagactatc gttcttattt taatatattt tcttactgta tacttttaca aatttttaac
     2341 atttatacac tcgagtaacc tcatttacaa acatagaatt taagtactat aaccatacat
     2401 aaacttatct attttttcat tgataaggca actctttttt tgtttaagtc aattccaatg
     2461 acttttacat ctactatatc acctactgta acaatactca tagggtcttt cacaaaacta
     2521 ttactcattt ctgatttatg aactaatccg tcatttttta ttccaatatc tacaaatgca
     2581 ccaaaatcta caacatttct tattgtacct tttaaagtca taccctcttg tatatcttca
     2641 atttttaaga catcagttct aagtataggt tttatacctt cttctcttgg gtctcttcct
     2701 ggttttttga tttcagttat gatatctttt aacgtaactt gccctacttc cagtttttca
     2761 cttaattctc tcaagccaat ttcttttatt ttttcatcta tttcacatat attcttattc
     2821 tttacatcat ctaaagaata tccaattatc tcaatcattt ttttacatat atcataactt
     2881 tcaggatgca cacctgtatt atctagtgga tttttaccct ctaagattct cataaatcca
     2941 gcacattgag taaatgcttg tggtcctagt ctctttactt ttttcaattg tgctcttgac
     3001 gtaaaatctc cattttcttc tctatatgct attatatttt ttgctatagt tttacttatt
     3061 cctgcaacat gttctagtag ggagtatgat gcagtattta aatctactcc aacactattt
     3121 acagaatctt caacaactcc atctagtact tcttctaatc tctttttgtt taaatcatgc
     3181 tgatattgac caacgccaat acttttaggg tctattttta caagctctgc taatgggtct
     3241 tgtaatcttc tagctataga tatagctcct cttattgata catttatatc tggatgttcc
     3301 tcatttgcaa gttcagaggc tgaataaaca gatgctcctg cttcactaac tatcacatac
     3361 tgcacttcgt tatctatttc ttttatcatt tctgatacaa aagtttctga ttctctagat
     3421 gcagtaccat ttccaattga aattatatct atatcgtatt tttcaatcaa tccttttaaa
     3481 actttctttg ctccctctac atcattttga gggtctgttg gatatactgt tgtataatct
     3541 agtagtttac catttttatc tactacagct attttacatc cagttctaaa tgcagggtca
     3601 aatcccataa ctactttgtc tttaactggt ggttgaagca ataaactttt aacattcttt
     3661 ccaaatacac ttatagctct ttcttgagct atttctgtta aatgatttct tatttctctt
     3721 tctattgatg ggaaaattaa tcttttataa gaatcttcta ttgaacttac gatttcttct
     3781 ttatttttga aatttttatc atttacatat tcgtttatta tgtaatttaa aactttatca
     3841 ttatttattt ctaacttaac ctttaggaag ttttcttttt ctcctctgtt tattgccaaa
     3901 actctatgtg gaggcatact ttttactgct tcactataat cataatacat gtcataaacc
     3961 gatttctcat cagtggcact tttactaact atcatacctt ctcttaaagc taattctctt
     4021 atatactttc taatcttagc atcatctgag actagttctg caattatatc tctagcacct
     4081 tttagagcat cttcaataga aagaacttct ttctcttcat caagataatt ctttgcctct
     4141 atttctatat tatctaaatt attctctagt attgacaatg ctaatttttc taatcctttt
     4201 tcttttgcta ttgttgctct agttcttttc ttttgtttat atggagcata tatatcttct
     4261 acttcttgta gtgtttttgc tttttctata ttttgtgtta tttcatctgt taatttacct
     4321 tgttcatcta ttagtctaac tacatcatct tttctacttt gaagatttct caaatacatt
     4381 aacttttcat aaaattctct tagtgtaaca tcactcattt cgccagtcat ttcttttcta
     4441 tatctagcta taaatggtat tgtatttcct tcatcaataa gttttaatgt attatttatt
     4501 tgttcatctc taagattaaa ctcttttttt aaaatttgat ttatatccaa tttattcact
     4561 cctaatgtta gtttttcata agtataacaa aacagaacga aggctgaggg ttatcgtcct
     4621 cagcctaagt gctgtaagtg catattaagc actaaccttc ttatacatat atcttaatta
     4681 cagtgcatat ttattaaact attttattca tttttaagta ttcattaata aacaagtcta
     4741 tattcccatt cattacacta tctacatttc caaactcagc atttgttcta tggtctttaa
     4801 ccaatttata tggttgaaaa acatatgacc taatttgact accccaagtt atttgtgagt
     4861 atttgccttg tatatcttct attttttcct tttgttctaa ctcttttaat tctatcaatt
     4921 ttgccattaa aagtctcata gctctgtcct tatttagatg ctgagacctt tcattttggc
     4981 actgaactac tacaccagta gggatatgtg ttattctaac tgctgaatca gttgtattta
     5041 catgttgccc tccagctcca gaagctctat atgtgtctat ctttaaatct gatggattaa
     5101 tttctacttc tatattttca tctaactctg gtgttacatc tatagatgca aatgaagtat
     5161 gtctttttcc tgatgggtca aatggagata ttctcactaa tctatgaact cctttttcac
     5221 tctttaaata cccataagca tttgttcctt ctacaagaat tgtagctgtt tttatccccg
     5281 cttcagggtc tgatattata tctaagagct taactttata acccttactt tctgaccatc
     5341 ttatatacat tctcaaaagc atttgagccc aatcttgagc atctaagcca ccagtacccg
     5401 catttataga taatatagca ttatttttat catactctcc atttagaaga gtctttattt
     5461 tcatttcaga taatttttct tccattgatt ctatggactt ttctatatct ctttctacag
     5521 aatcatcatc ttcttctaaa cctatttcaa ttaatacttc tatatcttct agtaagcttt
     5581 ttaagctttc atattcttct aatgtctctt ttaaagactt attttcttga agtactcttt
     5641 gagcgacttc attatcattc caaaagtctt gtttattcat cttttcttca ttaatttgta
     5701 tatttttcat caatgaagct atgtcaaaga gaaaccctca attcttctaa atttgaagcc
     5761 atctgctcca tactatgtct atactcttgt aactttaaca tttaatcctc cgagattatc
     5821 ttccacaaca gtttttatat tttttaccac ttccacaagg gcataagtca tttcttccaa
     5881 ccttttcatc ttttttaaca gttttacttg ttggaagtgt tccatctgat ggagaagata
     5941 gattttcaac atctacaact gcttttcttt ccactggagt ttctattgtt atattaaata
     6001 aatatcttac agtatcttct ttgatatgtt tattcatttc atcaaacata tcaaaacctt
     6061 ccattttata agcaataact gggtcttgct gacctacagc acgaagacca ataccttggc
     6121 gtaattggtc catagcatct atatggtcta tccaatggtt atcaacagct tgaagtaaga
     6181 ttactctctc aacttctctc attctctcat agccaacttg ttcttccttt gaagtatata
     6241 tcttcatagc tatttcataa actttttctg ttatttcaac tggattaagt ttttctattt
     6301 ctggtatttc tatgctacct tttggcaaga acagattata catatgttct ttaaatcctt
     6361 cttcatcaaa tcctttatct tgtgtataaa gagtaactgc ttcttctatt atagaatgag
     6421 tcattgattg tatttgttct tgtaaatctt caccttctaa tacacgtttt ctctctgcat
     6481 atattatttc tctttgttta ttcataacat catcatattg aagtacatgc tttcttattc
     6541 caaagttttt accttcgact ttcttttgtg ctccttcaat agacttagat aatattctat
     6601 gttctatcgg catatcttct tcaagaccta ttttatctac aataccagat attctatcac
     6661 taccaaacaa tctcataagg tcatcatcaa gacctatata gaatcttgaa ctccctgggt
     6721 caccttgacg tccagcacga cctctcaact ggttatctat tcttcgagat tcgtgtcttt
     6781 ctgtacctat tatagcaagt ccacctgctt ctacaacttg tttttgttct tcttgtgttt
     6841 gttgcttaaa cttttcaaat agtttttcat actcttctct agcttcaaat agaatttcat
     6901 taccttttac aggtatacct tctattggag tatctactct atttactatt tcttctttaa
     6961 atccatttct tctcatttct ctcttagtta aaaagtctgg atttccacct agaactatat
     7021 ctgtaccacg accagccata ttagtagcta ttgtaacagc tccaagtcta ccagcttgtg
     7081 ctattatttc tgcctcttta tcatgatgct tagcatttaa tacctcgtgc tttatgccct
     7141 ttttctttag tatttgtgat aataattctg atttttctat tgatactgta cctacaagta
     7201 ttggttgatt gactttgtgc ctttcaatta tttcttgtgc tacagcatta aatttaccta
     7261 tttcactttt gtatacacaa tctggtaagt cttcccttat cataagctta ttagtaggta
     7321 cttggaaaac atccattttg tagattgctt taaactcttc ttcttctgtt tttgctgtac
     7381 cagtcatacc agaaagtttt ttgtacattc taaaataatt ttggaatgtt acagtagcta
     7441 gtgttttaga ttctctttgt attttaagac cttctttagc ctctattgct tgatgtaaac
     7501 cttctgaata tcttctacca aacataagtc tacctgtgaa ctcatctact attactattt
     7561 ctccatcttt agcaacataa tcaacatctt tcttcattat tacatgtgct cttaaagctt
     7621 gatttatatg atggtataat tctgtatgag atatatctgt tatattatca acattaaaat
     7681 acacttctgc tttttgtata cccgatgctg ttagagatac agccttatct ttttcttcta
     7741 attcataatc atctggtttt aaggttaaaa caaatgtgtt tgcatctgaa tataagtgag
     7801 ttgatttgtc tccaggtcca gatatgataa gtggtgttct agcctcatct attagtatag
     7861 agtcaacctc atccactata gcataattta atcctctttg aactctttgc tctttgtgta
     7921 ttaccatatt gtcctttaga tagtcaaatc catattcatt atttgtacca taagttatat
     7981 cacaatcata ttgttctttt ctaacttttg gattttgacc gtgtataata acgcctacac
     8041 tcattcctaa aaactcatag atttttgcca tttggtctct atcacgtttt gcaaggtaat
     8101 catttactgt tacaacatgt acaccttttc cagtaagcgc attcaaataa acaggagcag
     8161 ttgcaaccaa agttttacct tcacctgttt tcatttctgc tattcttcct tgatgtagta
     8221 ctatacctcc aatcatttga actctgtagt gtctaagtcc taacactctc ttagaaactt
     8281 ctctaactac tgcaaaagct tctggaagta tatcatctat actttcacca tttgctaatc
     8341 tttctttaaa tatatttgtc atatttttaa gttccgagtc tgccatagat tcaaatttag
     8401 gttctaatga atctattata tcaacagttt tattgaactt ttttaattct tttttatctg
     8461 ccatgttgaa taaattatcc ataaagctca tattttacat ctctcctcgt tctttattgt
     8521 actattattc attttaccat aaaaactata cttttaatat taatttaaaa agctatcgat
     8581 aatttattta tcgatagcta atatgattaa atctaaattg ctttattcat gttctattat
     8641 accatatcca ccattattac gcttataaac tactgctatt tcatctgaat ctatgttctt
     8701 gaacatataa aaatcatgtc ctattaattc catctgtaat acagcttctt cttcactcat
     8761 tggcttaaca ctaaatttct tacgtctttg aattactaat tcttgatttt catccccata
     8821 atcctcttgt tcatcagcta gatttatctc tgcatcttgg aatcttatac tcttgttgtc
     8881 tttatgttta tcttgtaatc tctttttata tttttttaat tgtttactta atttatcata
     8941 tacaacatct atagctgtgt ataaattttc ctgcatatct tctgctctaa ttataggacc
     9001 atttacagta gctattgtta cttctatttt ttgtcttgct tttctcgcac ttactgtaac
     9061 ttttacatca gtgtctggat gaatgtaatt atctaactta cttagtttag tttcgatttt
     9121 attttttatt gcatcagtta attcgatttg ttttccagat ataattatgt tcataaatac
     9181 atctcccttc aattatgcca gtataagaaa atttatactt taataatata ttctacacga
     9241 actctcataa tcctcttttt attatttact tttatgattt tttctattca attgatagaa
     9301 ttatttatca tttttagtta cattgatact tatgtggatt aatattatta caaacacctg
     9361 tggataatgt ggataaaact gttaataact cttgtttttg tttatttaaa gctattttta
     9421 ctcctatttc tatccacaaa aatctccatt aattgtttat taatatgtga attcatgtca
     9481 taagatggta attttaggaa attatgtgta ttatatgcca atataccttt ataatttttt
     9541 aataaatata aaccctctaa aacctccata atctatcttt tctttcatta tttatattta
     9601 aaactactta aaagttataa agcatactca acaataattt catatttaat acctattaac
     9661 attcatatac ccaaattata attaacttac tctctttatt taaactaagt attttatatt
     9721 catatactat cttgaaatta tatttacgag ttgtttaagc ttttgtctat ctatatcttc
     9781 aaaaggactg ttatttattc taatcgctgt accaaaatga aaattacttg ccttagttaa
     9841 ttcctttatc ttttctatat tattaaaatt aagacctcca cctaaaagaa tttttatgtg
     9901 attagaacta agcatcatat tctttatcat ttgtatgtta tcaactatac ttcctttccc
     9961 tcctgatgtt aatatattcg taattttatc ataatccttc aaaattttaa cactatctat
    10021 tgtatttgat tcatctattg ctctatgaaa tgtaacatca agattatcac aacatttcaa
    10081 taaaacattt agatttttct catctatact attgttttta tccaatacac caaatacaac
    10141 tccattagca ccaatctctt taaccacact tatatctttt tgcattatac ttatatcttc
    10201 tttactgtat acaaaacttt tcgcatgatg tcttatcatt acgttcacag gtatctttac
    10261 actgttaaca acactttcta taagaccaaa acttggtgtc aatccacctt cagttaaagc
    10321 actaactaac tctattctat ctgcaccaca atcttctata attttagcgt cttctactga
    10381 cattcctatt atttctaaca taactactcc tttgctaatt catatattgc atttgcaaat
    10441 atctttgcat ttaatattat atcttctata tccatgtatt catcaggatt atgagctatt
    10501 cctttttgtc ctggaaatga aggtccaaac ggaactatat ttggcattac ttttgcatac
    10561 gtacctccat tagttgttac tggagttcca tctaatgatg taaccctttc ataagctagc
    10621 tgaagctttt taacaagatt actctcttta tcaaaatata caggattaaa attagaaatt
    10681 acctctatat cgatactatt tggaaaacta gcttttattt catccaatac atctttcaat
    10741 ttataactaa aatcgtattt tacctttata tctaatatag tatcactatt ttctatattc
    10801 agttctttat atgtaacaag cacattttca gacatatttt ctataaaacc taagtataca
    10861 tcttcttcat aattgatttt actttttatt ctaatctttg ccatgccttt ttcaccatat
    10921 acaacaggat atttacaatc tggtgtaaat cccataatag gtgctttttc tttttctaaa
    10981 taatatggta tatcttcaaa tccactttct tcatttgtac caaatattat tctaactctc
    11041 ttatctaatt ttaaatttaa ttctttaata gcatataatc catataaggc agacattata
    11101 ggtcctttat tgtctagtac tcctcgtcca taaattcttc cattagtttc ttctccctta
    11161 taaggctgat gcttccaacc atctccttca tgtacaacat ctacatgtcc aattacacaa
    11221 acataatcat cactatctcc atgctctgca taaccaatat aattatccaa gtttctaacc
    11281 ttaaatccca atttttctga tatttctaat gctttattaa gtgcctcccc tactttttcg
    11341 ccaaaaggga agccattttc tgactcccct ttgacactag gtatttttac aatatctata
    11401 atatcactta ataaatcttc ttttaactca tctattttat tacttatgat attcataaaa
    11461 atctccttat tataaaactc tattttaatt atgtaaccac tactttactc acttacttct
    11521 attggtgctt ttattctagc ctcatatgct tccatccact catttgatat aaccttatgt
    11581 atagtctttc catctactct atttgaaata tatacagtag gagattcttc tttagttact
    11641 actgaaaaag taaaatcagc tatatttgta gctactcccc attcaccttt attattcatg
    11701 gcaactaatg ataaatcacc agcttttccc cttcttttga taagctgttt atcaagttca
    11761 tttactgcaa tatcacatgc ttcttgtgga tgcttccctt ctttcataag tctaactatc
    11821 tcataagata tacatccctt cattaaatct tctcccaaac cagtagctgt agctcctcct
    11881 atactactat caacatagaa tcctgaacca gatagtgcag aatctcctat tctacctctt
    11941 ttcttcataa acaatccact tgttgatgta gctgcacaca tttttccagt tttgtctaaa
    12001 gatatcatac caacagtatc atgaccagca tatggactta atcctttgtc taaagtctct
    12061 ttttttctct ttttataatg taacttcgct ctatcagtca acatattttt tctttcaaat
    12121 ccatttttat gagcataatc ttcagcacct attccaacta aaaaattatt taccttctca
    12181 taacttaact tcctagctat gctgacagga ttagaataat ctcttatacc agcaactgct
    12241 ccaatatcta atgtgttacc atccataaat gcagcgtcaa gttctacctc acaattttca
    12301 tttggaagtc caccatatcc aaccgattta tagaatggat agtcttcaac tcttcttaca
    12361 gcaagctcta cagcatcacc tgcttccatg cctttttcta aatgttctga tgcctcagtt
    12421 attccttcta gggccattct ccatgtcgct atcattcccc acatgttaaa attcccctcc
    12481 ttaaattata tagattttac taatgtatgc ttattataaa tagtataata tttaaactta
    12541 ctaataatta atactcttat taataacaaa attcttcttt catcaaagtt atatcttctt
    12601 tatttgctat tgcttttgcc aatttacata tgttatttaa tgaatcatta agaccaattc
    12661 cacctttttt tggagtctta actatacata tgtcattttt atattcgttt atcacatcta
    12721 tattttcttc tgacatagct gcaaaagctg gtatatctgt tttatcattt atatttttag
    12781 acagtattgc tgacatctta gaaaagtttt cgtaattaaa gcttggtcca cagattacta
    12841 catcagggtt taattttttt accatagcta tcatcttact agttacttct tcttcattat
    12901 ttacaaaaaa ttcatcccca caaaataagg tagctattac cttagattca tttaaaaatt
    12961 gctccatcat tatacctggt cctaatgggc ttgattttcc agcaggaggt atattacttt
    13021 tctcttttcc tccagctcca gcttgtattt gatccaatat cataactatt ttcttcatca
    13081 tcttcctcct ataatactaa atcatcaaaa gatatatcat catcttcctc ttcaacagtt
    13141 gcattttctt ctcttaagta ttgcttatct agcattttta taaatggtat atacataaat
    13201 attcctagta taagaagcac tacttgtaag actgcacctt gccatcctgt tgttaaaaaa
    13261 ccagagaata taattggagt tgtccacggt aactgaactc catttgtaag aggcactaat
    13321 cctgctgaca tacagaaata agctattatt atatttattg ttggaactat tgcaaatggt
    13381 atcaatagta ttgggttaag cattattggt agaccaaata tcaatggctc atttatacca
    13441 aatattcctg gtaaaattga tagttttccc attgacttta tacgtttact tttacatatt
    13501 aataacatag ctatgactaa tgatagtgta ctacctgctc caccaaatgt cgcaaacata
    13561 tcttggaatt gacctgttat tatgtttggt atttttgaac cagcttgaaa agcagctaaa
    13621 ttttcagctg atagaatttt taatattgga ttatacacag ccccaacaac actacttcca
    13681 tttattccaa agaaccagaa taaatgtaag aatatataag cgattgccat agcacctaaa
    13741 ctatttccta attttaataa tggcatttgt aaaaacttaa atataaattc atgtatactt
    13801 ccatatggtg tagcttcaaa agctatctta actaaaaaga aaactaccat aactattgca
    13861 cttggtatta atgctgcaaa tgattttgat acggttggag gaactccatc tggcattttc
    13921 aatgtccatc ctttttttac aactgcagca aataatttta cagaggttat tgctgttatc
    13981 atacctacaa acattccctt agagcccatc caacctagtg gtatacctgt taccatgtag
    14041 actgattctg ttccttctgg tgtatatggt attacaaatg gtgttattat aaaaaaggca
    14101 actattgcaa ctgctgctga tgctattggg tctacatcta tttgctttgc ataagaatat
    14161 ccaattccta aaactgcaag tagagtcatt acttcaaatg ttgctacagt tggttgttgc
    14221 aattttacag cccaatctga accaaaaatt tgacctacaa attcattcca tccttttata
    14281 ggaaaatttg ctactaataa aaataatgaa cctattatta ataatggtga tgatattaaa
    14341 aaaccatctc taattgcaat taataattta tttttaccta ttttttcagc caatggcata
    14401 agtattcttt ccaatctatt catctatcta ccctcatttc ctattatttt ttactataat
    14461 tctacgcttg tttggatttt attagcttta tagcgagttt taatactttt tccccattca
    14521 tagttccata atcaacttca ttgattatat ctataagttt tccttgagct tcaaactttg
    14581 gttttaattc ctttagcatg tatttaactt gtggtcctag taatatacaa tctggatttt
    14641 tttcttcaac tattttgtca atttctgcta tcggaaatgc ctctacttct attggtaaat
    14701 tgtgttcatc tgctaccttc tgcattttat ctgcaagtaa acttgttgac ataccaaagc
    14761 tacaaaataa atatactttt cttttcattt tttaaatccc ccttaatttt tttattttaa
    14821 tttttgaatt tcttttctta acataatcat ttcttttata aggtttctct cactcataca
    14881 agtcatcaaa tggtcttgag agtgaatcaa taaaatagat atatctgatt tatctccccc
    14941 agcttccttt tgtataagtt cagtttgtac tttatgtgct tttaaaattt cttcatttgc
    15001 attttcaatt aattcatctg cttttttaaa atcgttttcc tttgcatagt caagtgcttc
    15061 ataaagcata gattttgcat ttccagcatg aattataatc tttaaaacta tttcttccat
    15121 tttttcctcc taagttgctt ctctactttt agattattta taccttacaa acatataata
    15181 tcttataaaa acaaaaaaac atgcacaact ttttccatca catggaaaat tttgtgctta
    15241 tccatagttc actatgaatt ttatataaga atatctttta ctttttcaat gtcacattta
    15301 cttagaatat ctagcatatt ttcatcctct gttaaattag atatttcttt aaataattct
    15361 ctaaatactt ttctatcatc atctttttta tatgctaaca aaagtataat ctttatatcc
    15421 tttccatttc taattcttat tggtgttttt aaccttgcaa aacataaaac agattgtttt
    15481 atatatttca tatctccatg agggaataat attccttttc caacattggt tggatatgac
    15541 ttttctcttg ttataataga ttgtaaatac tctttttctg cataatgttt ttcaattaat
    15601 aaattagtta aatatgttat agcttcataa atatcatcat gttctaactc tcttattaaa
    15661 taatcagaca tcattaatct gttcataagt ttatatgaag tgtctttttc ttttattttg
    15721 tttgtgaact ctttaagttt attctcatcc tcatcagtaa ataatggtgt tgttataaaa
    15781 attggttttt ggatattttc atactcttga aatgcaatta taaaatctat agtcttattg
    15841 aagttatctc tttttaaatc ttcctctctt aatgtttcaa taatttttat gttatcaaat
    15901 ctcttttcta tctttacttt taatagcatg cttactccaa aactaaatgg acatacaata
    15961 cttgcctttt tatttgattc ccttttgctg tattttcttt ctagagaggt ttgaaagtga
    16021 agtgtaagat aaccaatttc atcttctgtt atttgttttc cttttaattc aatttctatt
    16081 gaacttgata ttactgtaaa taaaaaacaa aatttagttt ttatgttatc taaaagtgga
    16141 ttttctaagt aaacattgct tttcatttgg tgcattgtaa cattcaaatg acatattaat
    16201 tgttcataaa gtaatatatc attattaaaa tttattccgg attcttctga tactaaattt
    16261 ataatttttt tgtataactt gctaattctt catttagata taaagatttc ttaatattac
    16321 tactatttat ttgtttgttc atacccaata ttaaagactc tataaacaat ttttcttcat
    16381 aatttaatac tatggataaa ttagatttca attcatcttc tatatctaat atcagcattt
    16441 ttagcttaag ctctttctta gtatttgata cttttaaaga atttcctagc cttattcgta
    16501 ttatatttat aagtaaaaat agcatcaatt gcttgaatga tatatctgta agtcttatat
    16561 ccatatcctc ctctatagta aacattatgt tttttacaat tattatatct actatatgga
    16621 agtactctat ttcatctatg tttaatctgt atttatctat gtatttaaat aagaatgata
    16681 ctaacattat tcttatgtct ttctcatccc ctccaatata tataccactg ccttttttag
    16741 tcgagacatt taaattataa ttatttaatg ccactgataa tttacttatt tcttctctga
    16801 caatattttt atttatgtac aacttattag ataaatcatt taaagtaact tttttattat
    16861 tacaaagtag taaatttaat atctcaactt gcttatgaag ctcactattt actcttttaa
    16921 cttcacctcg caacttctga tttaccagta ttttgtctgt tccatctcca ctaaatttaa
    16981 ttccaatatt aggttttctt ttgatataag cattacaaaa agtagataac cattcatcaa
    17041 tatatttgca atcatttctt attgtccttt ctgaacactc aaaatcattg gcaatcttat
    17101 taacatttat aaactcttct tttaatagca acgttgttaa taagttttcc tgtcttttat
    17161 tcaaacatat cacctcttta tgactcaacc taaatttatt atatataaaa aagagctata
    17221 cataaaggta tgctagctcc cttttaatac gatatttagc tgacctggtt tttgacccca
    17281 cactcgccta ctggagggtt cgcttgggtt cgccgcacta ctactctctc tcgattttat
    17341 tatcggtagg tcttacttct aagccgcacc atgatcatta acccaatttt tagtgttaac
    17401 ttacgtggat ccctttgcct gcgactggct tggactttca accacagacc taaatatcac
    17461 ttattcattt taatatttta aattaaaatt gtcaatatat aagattattc cataatataa
    17521 ttatcatttg cttttgtaag tagagtcaat acaaaaacct tattagcacc cgataacttt
    17581 aaaagtttag aaatttcgtt tgttgttaga ccagtagtaa atatatcatc tattaaaagt
    17641 atatttttat tatttattaa tttaacattg tctttaacaa caaatacatt ttttaattct
    17701 tcttttcgtt ctttcttatt caatttatat agcattcttg tatatttttt tctacttata
    17761 caatccaata atggaatatt tactatttta cttaaattaa atgctatttt ttgtgcctga
    17821 ttaaaacctc ttttatttag ccttttttta tgaagtggaa caaataatat ataatcaaat
    17881 tttatgtttt ctaaatataa tttttctttc attatttgag ctatatactt agccataaag
    17941 gttttttggt tatacttcag ccctagcact atcttttttg tcacatcatt gtattcaata
    18001 caagatattg atttatcaaa ataaaagtct ttattaaaac aataactaca ttcttcaata
    18061 aattcttttt caattgaatg acgtattatt ggttttccac acttcatgca accatcttgt
    18121 ataaaattca tttctttaaa acaactttta catattgaat aagtatttgt tttctttatg
    18181 gatttatcac aaattatgca acttatattc tcaggatata taaaatccaa acatttatta
    18241 atcatatttt taaaattctt aataaaatta tcattgcata ttttcatatt tattctatca
    18301 acagcccttc ttgcttaaat ttatttagct tatatcctaa atttgaatac ctttgattaa
    18361 ctctattatt ttttatcata tattcaagat atttcacatc tccaactaat acaactaatt
    18421 tttttgctct tgttactgct gtatataata aattacggct aagtaacata ggaggagccc
    18481 aagctattgg caatactaca actggaaatt cacttccttg gcttttatgt attgttgtac
    18541 aaaaactatg gtcaatttca tctaattcat catataggta tgatacaatt ttagtctggt
    18601 caaataatat ataaatagtt tttttatctt tatctatgtg ataaacatat cctatatctc
    18661 cattatagat accttctcca ctttcttttt ggtcttctgt ttcccatttt tttgtataat
    18721 tattttttac ttgcattacc ttatcgccaa ctctaaataa tcttttagaa aaactttctt
    18781 caactttaaa tttttctttt ttgttaagat acttctgtaa ctctatattg agattagtaa
    18841 ctcccaattc accttttctc attgaggata aaacttgtat atcttttaat ttatcaactt
    18901 tataaaattt aggtaatctt tcattaacca atcctattat ttcatttaaa atttcttcat
    18961 tagttgattt tcttataaaa aagaaatctt taccttttgt atttagataa agtggttctc
    19021 cattgtttat cttatgggca tttacaatta tcatactttc ttgcgcttgt ctaaatattt
    19081 catttaattt aactacattt ataatattag aattaatcat atccttaagt acatttccag
    19141 cacctacaga aggtaattgg tcactatctc caactaaaat tactcttgtt cctaacttta
    19201 tagctctaag taaattgtac attaagataa tgtctaccat tgatacctca tcaacaatta
    19261 ttacatctga atttattggg tcttcttcat ccttaaaaaa agttaaatca tcatctgttg
    19321 aaaatcccat ttctagcaac ctatgtatag tttttgcttc tttattagaa gtttcactca
    19381 ttctctttgc tgctcttccc gttggagctg ccaaagtcac gcttttacca ttattttcaa
    19441 atatatctat gatagcatta atggtagttg ttttaccagt tcctggacca ccggttataa
    19501 ttaatacgcc actattaaca gattctttga ctgctaaaat ttgtttttct gctaacttaa
    19561 ttttcttatc ttcttctaaa acattaatct cgttatctat atctattttt aagtcttcaa
    19621 attcatactg tgataatttt actatttgac tacatactcc attttcagct aaatagtatg
    19681 gcataaggta tataagattt tcattgttta ctttctctac atgtattttt tgattataaa
    19741 ctagcatcat tacacactct tttattattt ctccatttag ctccaataat ttttccgaat
    19801 cttgtattag aacatgctct ggtaaatatg tatgcccatt acttaaagat ttatttaaag
    19861 tgtataagat tccctgacaa attctatcct tagaattttt atctatacca attttatttg
    19921 ctatactatc agctacttta aatccaattc ctcttatatc ttcagcaagt tggtatgggt
    19981 ttttatttat aacttctatt gcactgcttt tatatttttt atatattttt aggcagtaat
    20041 ttggtgttat tccaaaagga gatagctgta ttattatatt tctcaattct ctgtcttctt
    20101 cataactttt tactatttgt tttacttttt tactccctat accttctact tcttgtagtt
    20161 tttctggtga attttgtatc acctctaacg tatctacacc aaacttatca attattcttt
    20221 tagccatttt ttcaccaata ccatgtatca ttccagaaga taagtaaaca taaattcctt
    20281 ctaaggatga tggtgttact ggcataaaac tattcacttc aaattgagtc ccataaatct
    20341 tatggtttac ccactttcct tcaacttcta tgctttctcc aattgccaaa gtaggcatac
    20401 atcctacaac gacaatttca tcattttcat ttaccagatg agctattgta taaccattat
    20461 cttcgttttt aaagactatt tcacttatca ttccttctaa tttttccatt tttatctcct
    20521 aaaaatacta ataatcgctc catttaaatg ttagtataat tttatatatt aaaactatga
    20581 taatcgtaga aaagttttat atttttatac ttaacgtaat ttccaactat tatacatttt
    20641 atcagaaaca aaaaaaatct gcatccataa aatatggtac catgaaatac aggtaccata
    20701 tttaaatagc tattttattt taattactcc atatttagag tataaattaa atttcataag
    20761 tatattacag tactagctta tttcagattt attctccttg tacccctcag ttttagtaat
    20821 tcttctataa agcatacata gtaaaattaa aattcccatg taacctgtat atggatatag
    20881 tgtacctact aacttatcaa atggcagtag acctcctata caagctaata tacttactac
    20941 tattgttata attttaaatt tagggtggtc atcttctaca aatcggttag ttactgacca
    21001 taacaatggt acagctgtag tgtatattcc tagtaaaagt actactgaga ataaaatacc
    21061 tattattgga gatatcttat cagctaagta aagtgctggt atctcttttg tgtatatatt
    21121 tcctatgtct gaaagtaatg ccaaattcat cattatagct gctgccataa gtgctactcc
    21181 acctactata ccaccccaca cagcatcttt cttatttact gcagaagctc ccattcctgt
    21241 caagaatgtt attactatta ttacattata acaagtataa agtactcctg agaaatatcc
    21301 atttgatgta gcagttttaa tatttaaact atcaaccata tttccagcat ttgataaagc
    21361 atcaatattg ctaaataaac taattgctcc tactaaaagt gtaaatatta ttattatagg
    21421 tcctatatta cctagtattt tagaaagcct tgtcaaacct aatgaaacag ttattaggga
    21481 aacgattgtc ataccaatcc tacctacata tggatttaat ccatagtact ctgataatgt
    21541 agctcctgct cctgcaatca ttattactaa tgttccaaat aaaaacaatg gaccaaacca
    21601 ttcaaaaaat gttccaagat attttccaca atatacttga taaattttta ttggctcctt
    21661 aagtttcaat tctcttccct tatctataac ctctgctcca aaccatgaaa atagtaccat
    21721 agagattatt ccacctataa taccagcata tccataaaat gtaaaaaatt gcagtatttc
    21781 ttgaccagtt gcaaatccag aacctattac agtagcaaca taagctccag caaaacttat
    21841 aaccgtttta atatttactt tgcctgacat acactactcc ccccaattaa aattattttt
    21901 aaagtgtaag tttattaaga ttatattaga ctcattatta tgatatttca atatattttt
    21961 aattatttaa cattagttaa cacattctta accatccttt aacatatatc ccataatatg
    22021 cataaattta gtatagtatt atataaaatg tattgaccag attaaatact gtttattcat
    22081 tatatgatga taaattaaca tttattaaaa atatttaact cataataaaa aagctgttag
    22141 aaagtaatcg taaaattaca ttctaacagc tttttagtta aaaattattc acctaaagca
    22201 tcttttctta actgctcaac cttgtcagtt ctttcccaag gtaaatctat atcagttcta
    22261 ccaaagtgac cataagcagc aaccttttta taaagaggct ttcttaaatc taagtctctt
    22321 attatggctc ctggtcttaa atcaaagtgt ttatttacta attcaacaag tttttcttct
    22381 gaaactttgc ctgtcccaaa agtatctata aatatagata atggtctagc tatacctata
    22441 gcatatgcaa gttctatctc acatttgtct gcaagaccag ccgcaacaat attttttgca
    22501 acatatctag ctgcataagc agcagatcta tcaacctttg tagggtcttt tcctgagaaa
    22561 gctcctccac catgtctaga atatccacca taagtatcta ttattatttt tcttcctgta
    22621 agacctgtat ctccttgtgg tcctcctata acgaatcttc ctgttggatt tatataaatt
    22681 ttagtatctt catcaagtaa ttctgctggt ataacttctt taataacatg attaattaaa
    22741 tcttctctta ttttatcatt tgatactgtt tcacaatgtt gagctgatat aagaactgta
    22801 tgtactctta cagctttact tccttcatat tcaacagtaa cttgagtttt tccatctggt
    22861 cttaaataat caactagacc agtctttcta acctctgtaa gtcttcttga taatttgtgt
    22921 gccaaagata ttggaagagg cattaattct ggagtctcat tacatgcaaa tccaaacatt
    22981 ataccttggt ctccagctcc aactttttct atttcttctt ctatctcttc tccagtctta
    23041 ctttctaggc cttcatcaac tcccatagct atatctccag attgttcatc aatagaagtt
    23101 ataactgcgc aagtatcaca atcaaatcca tattttgctc ttgtatatcc aatttctctt
    23161 actgtttctc ttactaattt aggaatgtct acataagcag atgtacttat ttctcctgct
    23221 actaaaacta aaccagtagt aactgtagtt tcacaagcta ctctagattg tgggtctttt
    23281 tctaataatg catctaatat tgaatctgat atttggtcac atatcttatc aggatgtcct
    23341 tctgtaactg attctgacgt aaataaatgt cttgccattt ttattccccc ttatttattc
    23401 ttagttttta ttataggttt taccttattt ttaattttag aaatttactc aaaataaaaa
    23461 acctctcctt tataagaaga ggttaataat cgtaattaca tactaattaa ctgacctcat
    23521 ctttcaagac ttttgtcttg taggaattag caccttttcc catttgggtg gttgctgggc
    23581 ttcacagggc ctatccctca gcctctcttg ataaggtaat caagatttaa gttttgtttt
    23641 actttactag cttactattt ttttaaaatt ttgtcaatat ttttagtcat attttttcta
    23701 ttcctttttt tgtaagacaa ataaaatttt ctatctatct gaaatttaaa gttaggtctt
    23761 tttaatgctt tttattcata tatttttttg aagggggtgt tcctattgta tttagctaaa
    23821 gtaaattaca gcgatgatga tgttatacag atgctaagtt cagttctaaa aactataatt
    23881 aatgaaaata cgattgttgt ttgcatagga acagacaggg ctataggtga tactttaggc
    23941 cctttagttg gaacaatact taagaacagt aactttaaat accccgttta cggtacacta
    24001 gataacccta ttcacgcctt aaatatatat gagtctttag atactataaa aaatacacat
    24061 atacaaggta acttcttggc tatagatgct tgcttggggt cgcagagtaa cataggtaat
    24121 atacagatta gagaaggacc tatcctgccc ggaaaaggtg ttggcaaaaa actacctcaa
    24181 ataggaaatt attctatagt aggaattgta gataaaattg atgaaaataa taaactttca
    24241 tttaataata tacgtcttag ctttatatta gacttagctg aaacaatagc cttggcttta
    24301 ttagtatcca cctaatctaa atataaacta aatgataaaa ttctttatat aaaaaggtag
    24361 atattttaga gtatctgatt aaatactcca aattatctac ctttttactt ttatatttca
    24421 agtttttatt tttctactaa ggcaaacatt aattcccctt tacagacaag ttcttcacca
    24481 acatatgcct tagcatctgc cttggctata tttcttctaa gagttgtcat ttcaacttcc
    24541 atagttaatg tatctcctgg tcttacttct ctcttgattc taactttatc tatacctgca
    24601 aatacaccta gttttccttt gttttcctct acagacatca ttgccactcc acacacttgt
    24661 gctaaagctt ctactattaa tactcctggc attaaaggat attctggaaa gtgaccttgg
    24721 aaaaatggtt cattaactgt tacattcttt ataccaacag ctcttttccc aacttctagc
    24781 tctgtaatct tgtccactaa taaaaaagga tatctatgtg gtattaattt ttttatttca
    24841 tctatattta acattctaaa ctctcctaat tattttcttt tgaaagaccc gccagtttca
    24901 agtaggtcta ttgaacctaa agctattcct gtaccttttg caacacaaga taatggttca
    24961 tctgctattg taacagttat tcctgttctc tgttctatta ttttatctaa tccatataat
    25021 aatgaacctc ctccagtcat tattatacca acatctccta tatctgctgc taactcagga
    25081 ggcgtttttt ccaaaacagc atgagtagca actactattt gctcaacaca ttctctaagt
    25141 gcttccaaca tttctgttga atttatagtt atagaactag gaagtcctgt aattaaattt
    25201 cttcctgtta tcttcatata tttttcttct tctctcttaa atgcagaacc tatttcaaac
    25261 ttgattttct ctgctgttct ttctcctata agaagattgt gttgtttttt catatattta
    25321 actatagcat catcaaatct atctccacca acctttatag attcacttac aacagctcct
    25381 cctagagata ttacagctat atctacagta cctccaccaa tatctataat catattacca
    25441 ttaggctgtg atatgtctac accagctcct atggctgctg ctattggttc ttctattata
    25501 tatacttctc tagctccagc ttgtctcgta gcatcttcta ctgctctctt ttccacttct
    25561 gtgacacctg ttggtacgca taccataatt cttggcataa aaaatcttcc aaatcccttt
    25621 ttatcaacta tcttatctat aaaagatttt aacatcttct cagttacatc ataatctgaa
    25681 attacaccat ctcttaaagg tcttatagcg actatattac ctggtgttct ccctatcatc
    25741 tttttagctt cttctcccac agctaaaaca gaatctgttc tgttgtctat cgccacaaca
    25801 gacggttctt ctaaaacgat tcccttacca ctaacataaa caagcacatt tgcagtacct
    25861 aaatctatgc ctatatcagc tccagccatt ttttcaactc ctttatttat aatattagat
    25921 tatattataa gcctatttta atactcctat aataaagcat atacaaaaat ctcgctttaa
    25981 tcaagagaaa ataaatcttt tgagagtatt attattttgc tatttataat tacttaatat
    26041 atttcttttt ataatttaat taaataaaaa aagtaggttt ttaacctaca tttttttatg
    26101 ttccttctca tattttaatt tcgttgctaa tccccctcta atatgtctct ctgatttatt
    26161 tttgtccagc acatttttaa cttctttggc aagggatgga tttatttcct tcaatctttc
    26221 tgtaacatcc ttatgaattg tacttttact tactccaaat gtcttggcgg tttgtcttac
    26281 tgtagtgttt ttttcaagta tatattttgc taccactatg gccctttcct ctatatgaga
    26341 tctcaaagat tacccccctc acataatagc tttaatgata tatatgtaaa aacaccaaaa
    26401 aatataacaa gcttaaccaa atatgattaa acttgttata cttttactta attagactca
    26461 ttgggtctat agatttttct ccactatacg cctctaaatg aacatgtata ccttcttcac
    26521 tttctatttg agaagtacta cctacagtac ctagagattg tccttctgtt accttctgtt
    26581 ctttcttaac actaacattt ttatctagat ttgaatatac tactacaatg ttattgtcac
    26641 tttttatttt gactgattgc ccatattctt catcattaaa tacatctact actattccgt
    26701 ttaatagaga ttttacttct ttgcctttag tacaactaat atctacacct ttatgagttt
    26761 cccaaacatc aagtgtttta gaataactag gttctttttc tgaatatcct cttattactt
    26821 tatctccaat gtaatttaat tttgttgtac tcgatttatt ttcctctttt gacttagctt
    26881 tttctaagtt ttgttttgaa tccgttgcag tagggatagc atctttcttg tctttttcaa
    26941 ttaaatgtat ttcctcttca ctatctttat tagcattttc cataattcct ttattagaag
    27001 ccaatttatc tacattatta tttgtaaacc aaactccacc tactgctaat aaacaaacac
    27061 atacaaacaa agataaataa aaaccatctt tttctaacag ctttttcttc attttgcacc
    27121 tccaaatata tttctacatt tggattattg acgtgttttt ggcattttat acaattatta
    27181 gtatatatct tttatttttg tatctgtata gtaatgtgat aatatatcat aatatttata
    27241 gccctcttct gccattcctt ctgctcccca ctggctcata ccaacaccat gaccatatcc
    27301 ttttaccacg aaatctatgt atccttctcc aaattttatg tcaaagtttg cagagttcaa
    27361 tttaaaaaca gttctaatgt cttttccagt caattcttta tttcctaatt ttattttttc
    27421 tacagttccg gcatcacttc ttttagttat tgaaacttgt ttacttaaat tattcacatc
    27481 tatcacaata gtgctatatg cccttctaag acttttaaca aaatctgtgt tgcttatttt
    27541 aagtgttgac gcaaatttag gggaatactt atcataagga ctttccacag actttaaata
    27601 aggatatttt gcagaaaata cttcttcact attttctgtt tttcctgaag atgtagaaaa
    27661 ataaagagga agtatcgcct tatcgttata agttattatt tgacctttag ttcctctaac
    27721 tgcttcttgg attttggaat atttattctt aatccattct tcaccattta attttttaag
    27781 agtatcataa ctcttatatt cttgacaatg tttataatca gtacatacta ctgcattttt
    27841 atgtttacta gatttaccat gttcctgttt atatactaca tatgttctag ctgctacaga
    27901 ttgagctttt aaggcttcta tatcaaactc tgaagacatc tcaccagcta gtactccaca
    27961 taaatagttc tccatgtcca tcttttcggt tttccctgtt atatgattat atacattgat
    28021 aattggtgct tttttattta cagtttcata gttcactgta tttttttctt ctttattacc
    28081 tttgtcttca atatcacttt ttttaatagt tttgtttata gatactgggc tctctgcttt
    28141 ttcagttaat tctacatttt tataagaaac taaagttata agtgatggaa ctaacacaga
    28201 gcaagtcaca aatcctaaca aaacaaccaa tgggttcttc atatactctc ctccttagct
    28261 tttaaaacct tgtatttaag tatatgaaca accctattaa tttataactt tcttctataa
    28321 accaattatt aatcttctac tatttcgatt tgaccaccta atgctgttat cttcttatct
    28381 atatcaacat agcctctttg gatatggtat atttctccta tttgagtttc tccttctgct
    28441 ataagaccgc ataaaataag ggctgcccct gctcttaaat cagtagcgtt tactgctgca
    28501 ccatgtagct catctacacc attaacaacc gcacttcttc catctatttt tatattagca
    28561 cccattctat taaattcagc aacatgcata aatctgtttt caaaaacagt ttctataact
    28621 acaccagttc cattagctac agtaagcata gccataaatt gtgcttgaac atctgtagga
    28681 aatcctggat gtggtaaagt ttttatatct attggtttta atacttttgg acctacaact
    28741 cttacagaat tgtccatttc tgttatttca caaccagctt ctcttaattt agctacaact
    28801 ggttttaaat gctccattaa aacattttca acagttatat caccttttgt catagctgct
    28861 gcaaccatat aagttgctgc ttctattctg tctggaatta cattatgttc tgctcctttt
    28921 aactctttaa caccttttat ttttatagta ttagtacctg cacctttaac atctgctccc
    28981 atttcgttca agaagtttgc taaatctact atttctggct cttctgctgc attctctatt
    29041 attgtagtcc cttctgctaa agaagctgcc atcattatgt tttcagttgc tccaacagat
    29101 gggaaatcta aatataattt atttcctact aatttttctg ttgctgcttc aacaaaacca
    29161 tggtccattt ctatctttgc acctaaggct ttaaatcctt ttaaatgtaa atctataggt
    29221 cttgttccta tagcgcatcc tcctggcata gaaattttcg tactattaaa tctagcaagc
    29281 aatggcccca taaccaaaaa agaagctctc atctttctta caagctcata tggagcttca
    29341 caagttttta tattacttgc atcaacagta agtgtatttt ctttatattc aacttctgcc
    29401 cctacatgtc ttaataaatc tgatataaca tgaacatctc ttaaatttgg tactccattt
    29461 aatgttgatt tcccgtttgc taataaagtc gctgctatta ttggtaaaac tgcatttttt
    29521 gcaccatcta ttttaacact acctttaagt ggattactct tttttactat tatcttagcc
    29581 atattttttg atctcctcat aattaataat ctaattttat aattggtgtg gcaatatata
    29641 tgagtgttct tccgtcgtcc tcattatatt taattcctat atttaaatta attttttccc
    29701 ctaaatattc taattcataa tcatttaata ctttactata tgctgttata gataaaaagt
    29761 ttacgtcttt cacttcatct atttgcttag cactcatatt atataatatt ttttgtaaaa
    29821 tatcatcata tttgttgttt tgtaaccttt ttgtgtattc tccaacaata caagcagaca
    29881 catctacttg agatgaatat ttatttaaca cattttctac gttactataa ataacctctt
    29941 tatgtttata tactttgtta tccaatatgt caactactat gtaactttct ttgttatttt
    30001 tatttattcc ttttatagaa atttctttat cgctaccttt tatagtagca cataattgta
    30061 ttttatcg
//
LOCUS       DACULQ010000028        27296 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.028, whole genome shotgun sequence.
ACCESSION   DACULQ010000028 DACULQ010000000
VERSION     DACULQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.028"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..462
                     /locus_tag="JA154_05245"
     CDS             <1..462
                     /locus_tag="JA154_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995264.1"
                     /translation="DMVSVKDYEINHLEEIVFDFDSRRSRDVGTIVRFKISNNSKYSY
                     KLNSAKMKFENCVENKKGMNKCSAYVNVDLFNEKNSITERTLKYGIKPNNQGYVDFVI
                     PKGLNFDYRYFNESGMSVEYKGEYIVNIPMAKGLYLVVGKNNDTWNANIME"
     gene            464..541
                     /locus_tag="JA154_05250"
     CDS             464..541
                     /locus_tag="JA154_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995265.1"
                     /translation="MKNKSIIDIIKNNMKFIKVQTRRDI"
     gene            541..1008
                     /locus_tag="JA154_05255"
     CDS             541..1008
                     /locus_tag="JA154_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HAT7995266.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            1717..2853
                     /locus_tag="JA154_05260"
     CDS             1717..2853
                     /locus_tag="JA154_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT7995267.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            2912..3694
                     /locus_tag="JA154_05265"
     CDS             2912..3694
                     /locus_tag="JA154_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HAT7995268.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            3713..4723
                     /locus_tag="JA154_05270"
     CDS             3713..4723
                     /locus_tag="JA154_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAT7995269.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            4742..5539
                     /locus_tag="JA154_05275"
     CDS             4742..5539
                     /locus_tag="JA154_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAT7995270.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            5637..6482
                     /locus_tag="JA154_05280"
     CDS             5637..6482
                     /locus_tag="JA154_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HAT7995271.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            6569..7744
                     /locus_tag="JA154_05285"
     CDS             6569..7744
                     /locus_tag="JA154_05285"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HAT7995272.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            7940..8842
                     /locus_tag="JA154_05290"
     CDS             7940..8842
                     /locus_tag="JA154_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAT7995273.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEDLINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            8847..9860
                     /locus_tag="JA154_05295"
     CDS             8847..9860
                     /locus_tag="JA154_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAT7995274.1"
                     /translation="MIYMIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKR
                     IAKDEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESC
                     DLAICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGA
                     LISLSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWIT
                     NALSNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDES
                     FSIDKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFY
                     EEK"
     gene            complement(10004..10231)
                     /locus_tag="JA154_05300"
     CDS             complement(10004..10231)
                     /locus_tag="JA154_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAT7995275.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            10403..10828
                     /locus_tag="JA154_05305"
     CDS             10403..10828
                     /locus_tag="JA154_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995276.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            10876..11082
                     /locus_tag="JA154_05310"
     CDS             10876..11082
                     /locus_tag="JA154_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995277.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            11264..11443
                     /locus_tag="JA154_05315"
     CDS             11264..11443
                     /locus_tag="JA154_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995278.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            11477..11677
                     /locus_tag="JA154_05320"
     CDS             11477..11677
                     /locus_tag="JA154_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995279.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            11930..12946
                     /locus_tag="JA154_05325"
     CDS             11930..12946
                     /locus_tag="JA154_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAT7995280.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(13069..13509)
                     /locus_tag="JA154_05330"
     CDS             complement(13069..13509)
                     /locus_tag="JA154_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995281.1"
                     /translation="MNIIQYIFIERGFFMKKLDTESLEKMAKQKNIDKDKIEKMADSY
                     KGKSENELMEELIKIGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTK
                     PAKTKKAKPTKGKSNSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            13679..14584
                     /locus_tag="JA154_05335"
     CDS             13679..14584
                     /locus_tag="JA154_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995282.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            14699..14879
                     /gene="ssrS"
                     /locus_tag="JA154_05340"
     ncRNA           14699..14879
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JA154_05340"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            15062..16279
                     /gene="cdeC"
                     /locus_tag="JA154_05345"
     CDS             15062..16279
                     /gene="cdeC"
                     /locus_tag="JA154_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HAT7995283.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(16379..17710)
                     /locus_tag="JA154_05350"
     CDS             complement(16379..17710)
                     /locus_tag="JA154_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAT7995284.1"
                     /translation="MIFLKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLG
                     IYHMIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALII
                     SIVASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQ
                     IIEQIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYI
                     ISIKDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGI
                     VSGMVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSS
                     IFFYFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTI
                     TGMLIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(17752..18546)
                     /locus_tag="JA154_05355"
     CDS             complement(17752..18546)
                     /locus_tag="JA154_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAT7995285.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            18647..20101
                     /locus_tag="JA154_05360"
     CDS             18647..20101
                     /locus_tag="JA154_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAT7995286.1"
                     /translation="MINLEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPI
                     RKLSNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQR
                     EDLIHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSD
                     GNIKTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISI
                     PMLDDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYII
                     EDDFISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLW
                     AKYSSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKV
                     RKSCGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSM
                     EELEKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            20130..20339
                     /locus_tag="JA154_05365"
     CDS             20130..20339
                     /locus_tag="JA154_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAT7995287.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            20359..21753
                     /locus_tag="JA154_05370"
     CDS             20359..21753
                     /locus_tag="JA154_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087556.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAT7995288.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            22020..22745
                     /locus_tag="JA154_05375"
     CDS             22020..22745
                     /locus_tag="JA154_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7995289.1"
                     /translation="MDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLPSEGDLCKEF
                     NISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSCHRINAKPST
                     KIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRIDYMFLLKEE
                     LEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKVRQSVYTENN
                     DVLYYNEQFIRSDKYKYAVSAEI"
     gene            22771..23184
                     /locus_tag="JA154_05380"
     CDS             22771..23184
                     /locus_tag="JA154_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HAT7995290.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            23188..23931
                     /locus_tag="JA154_05385"
     CDS             23188..23931
                     /locus_tag="JA154_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HAT7995291.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            23931..24401
                     /locus_tag="JA154_05390"
     CDS             23931..24401
                     /locus_tag="JA154_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIB"
                     /protein_id="HAT7995292.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            24412..25176
                     /locus_tag="JA154_05395"
     CDS             24412..25176
                     /locus_tag="JA154_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter"
                     /protein_id="HAT7995293.1"
                     /translation="MKMEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALG
                     NLHTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPI
                     ATLVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGF
                     SLGVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVA
                     YLNITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            25160..26002
                     /locus_tag="JA154_05400"
     CDS             25160..26002
                     /locus_tag="JA154_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAT7995294.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(26162..27067)
                     /locus_tag="JA154_05405"
     CDS             complement(26162..27067)
                     /locus_tag="JA154_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT7995295.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT        10712 a         3014 c         4958 g         8612 t
ORIGIN      
        1 gatatggtaa gtgtcaaaga ttatgaaata aatcatttag aggaaattgt ttttgatttt
       61 gatagcagga gaagtagaga tgttggtact attgtaagat ttaaaataag caataatagt
      121 aaatatagtt ataaattaaa cagtgctaaa atgaagtttg aaaattgtgt ggaaaataaa
      181 aagggaatga ataagtgttc tgcatatgta aatgtagatt tatttaatga aaaaaattct
      241 ataactgaaa gaactctgaa atatggaata aaaccaaata atcaaggtta tgttgatttt
      301 gtaattccaa aaggactaaa ttttgattat aggtacttta atgaatctgg tatgagtgta
      361 gagtataaag gagaatatat tgtcaatatt ccaatggcaa aaggacttta tctagtagtt
      421 ggaaaaaata atgatacatg gaatgctaat atcatggaat aatttgaaaa ataaatcaat
      481 tatagatata attaagaata atatgaaatt tataaaagtt cagactagga gggatattta
      541 atgtcaataa gtaatgtaag agagtatttt aaacaatttg gcaaggaaga cagtatatta
      601 gaatttgaac aatctagtgc tactgtggaa cttgcagctg aagcagctgg ggttattcca
      661 gcaagaatag ctaagacact ctcatttaaa ataggagatg atgcaatttt aattgtgaca
      721 gctggtgatg ctaagattga taataagaag tataaggctg aatttaattg taaggctaaa
      781 atgttgacac cagaagaagt cttggagttt actggacatg ctattggtgg agtctgtcca
      841 tttggtttaa aaaattctat aaaagtctat ttagatgatt caatgaaaag atttgataca
      901 gtatttcctg catgtggcag tagtaactct gcaatagaac ttacatgtga agaaatggag
      961 aagttttcta agtgtgaaaa atgggtagat gtttgtaaga attggtaaaa aaatatagta
     1021 aactatagta ggaattagta aaaaactacc tctgtataca atctatacag aggtagtttt
     1081 tttgtgtaaa tatttataaa tctaagagga aaatctaatt taaactaata atccaaaata
     1141 aagttaaaaa attctgattt tttttcgaaa caataaaaaa atgacaaaaa agtggaaaaa
     1201 atgtaataaa tattgttaaa aaaataatta gaaattattg aaaaattatc ctttgagtga
     1261 tactattata gatgtaagat atttaaaaat aaaaaatagt cagataaaaa tacgattatt
     1321 tagctctatt aaaataattt ttatacgata aaaatcttct gtacatggct aaaaaacaga
     1381 tgtacagaca atatttaatg tttaatatac cctaatataa atatcgtaaa caaattattt
     1441 tatctgtaac cagtgtgaaa gaaatcttat tctaacttga aatttcacaa aattccaaaa
     1501 agcgagattt tttttaagta tagttgattt taaattaaca atttatgatt aaattaatgt
     1561 cataattggt tagtataatc acatataaat atagagcgta gtttgattaa ggttataaat
     1621 ttctcgacgt atattcaaat accttattat agatgttgcc gagattttaa ttaataaatg
     1681 aaaataaaat atttataaaa tactgaggag gctattatgg atttaaattc taaaaaatat
     1741 cagatgctta aagagctata tgtaagcttc gctgaaaatg aagttaaacc tttagcaaca
     1801 gaacttgatg aagaagaaag atttccttat gaaacagtgg aaaaaatggc aaaagcagga
     1861 atgatgggta taccatatcc aaaagaatat ggtggagaag gtggagacac tgtaggatat
     1921 ataatggcag ttgaagaatt gtctagagtt tgtggtacta caggagttat attatcagct
     1981 catacatctc ttggctcatg gcctatatat caatatggta atgaagaaca aaaacaaaaa
     2041 ttcttaagac cactagcaag tggagaaaaa ttaggagcat ttggtcttac tgagcctaat
     2101 gctggtacag atgcgtctgg ccaacaaaca actgctgttt tagacgggga tgaatacata
     2161 cttaatggct caaaaatatt tataacaaac gcaatagctg gtgacatata tgtagtaatg
     2221 gcaatgactg ataaatctaa ggggaacaaa ggaatatcag catttatagt tgaaaaagga
     2281 actcctgggt ttagctttgg agttaaagaa aagaaaatgg gtataagagg ttcagctacg
     2341 agtgaattaa tatttgagga ttgcagaata cctaaagaaa atttacttgg aaaagaaggt
     2401 caaggattta agatagcaat gtctactctt gatggtggta gaattggtat agctgcacaa
     2461 gctttaggtt tagcacaagg tgctcttgat gaaactgtta aatatgtaaa agaaagagta
     2521 caatttggta gaccattatc aaaattccaa aatacacaat tccaattagc tgatatggaa
     2581 gttaaggtac aagcggctag acaccttgta tatcaagcag ctataaataa agacttagga
     2641 aaaccttatg gagtagaagc agcaatggca aaattatttg cagctgaaac agctatggaa
     2701 gttactacaa aagctgtaca acttcatgga ggatatggat acactcgtga ctatccagta
     2761 gaaagaatga tgagagatgc taagataact gaaatatatg aaggaactag tgaagttcaa
     2821 agaatggtta tttcaggaaa attattaaaa tagataaata ttaaaatata cacaataggt
     2881 cgatattaaa atagatatgg aggaaggatt tatgaatata gtcgtttgta taaaacaagt
     2941 tccagataca acagaagtta aactagatcc taatacaggt actttaatta gagatggagt
     3001 accaagtata ataaaccctg atgataaagc aggtttagaa gaagctataa aattaaaaga
     3061 agaaatgggt gctcatgtaa ctgttataac aatgggacct cctcaagcag atatggcttt
     3121 aaaagaagct ttagcaatgg gtgcagatag aggtatatta ttaacagata gagcatttgc
     3181 aggtgctgat acttgggcaa cttcatcagc attagcagga gcattaaaaa atatagattt
     3241 tgatattata atagctggaa gacaggcgat agatggagat actgcacaag ttggacctca
     3301 aatagctgaa catttaaatc ttccatcaat aacatatgct gaagaaataa aaactgaagg
     3361 tgaatatgta ttagtaaaaa gacaatttga agattgttgc catgacttaa aagttaaaat
     3421 gccatgcctt ataacaactc ttaaagatat gaacacacca agatacatga aagttggaag
     3481 aatatatgat gctttcgaaa atgatgtagt agaaacatgg actgtaaaag atatagaagt
     3541 tgacccttct aatttaggtc ttaaaggttc tccaactagt gtatttaaat catttacaaa
     3601 atcagttaaa ccagctggta caatatacaa tgaagatgcg aaaacatcag ctggaattat
     3661 catagataaa ttaaaagaga agtatatcat ataatttaag gggggataaa gaatgggtaa
     3721 cgttttagta gtaatagaac aaagagaaaa tgtaattcaa actgtttctt tagaattact
     3781 aggaaaggct acagaaatag caaaagatta tgatacaaaa gtttctgcat tacttttagg
     3841 tagtaaggta gaaggtttaa tagatacatt agcacactat ggtgcagatg aggtaatagt
     3901 agtagatgat gaagctttag cagtgtatac aactgaacca tatacaaaag cagcttatga
     3961 agcaataaaa gcagctgacc ctatagttgt attatttggt gcaacttcaa taggtagaga
     4021 tttagcgcct agagtttctg ctagaataca tacaggtctt actgctgact gtacaggtct
     4081 tgcagtagct gaagatacaa aattattatt aatgacaaga cctgcctttg gtggaaatat
     4141 aatggcaaca atagtttgta aagatttcag acctcaaatg tctacagtta gaccaggggt
     4201 tatgaagaaa aatgaacctg atgaaactaa agaagctgta attaaccgtt tcaaggtaga
     4261 atttaatgat gctgataaat tagttcaagt tgtacaagta ataaaagaag ctaaaaaaca
     4321 agttaaaata gaagatgcta agatattagt ttctgctgga cgtggaatgg gtggaaaaga
     4381 aaacttagac atactttatg aattagctga aattataggt ggagaagttt ctggctctcg
     4441 tgccactata gatgcaggtt ggttagataa agcaagacaa gttggtcaaa ctggtaaaac
     4501 tgtaagacca gacctttata tagcatgtgg tatatctgga gcaatacaac atatagctgg
     4561 tatggaagat gctgagttta tagttgctat aaataaaaat ccagaagctc caatatttaa
     4621 atatgctgat gttggtatag ttggagatgt tcataaagtg cttccagaac ttatcagtca
     4681 gttaagtgtt gcaaaagaaa aaggtgaagt tttagctaac taatttcttg gggagggtat
     4741 tatgagtaca agtgatgtta aagtttatga gaatgtagct gttgaagtag atggaaatat
     4801 atgtacagtg aaaatgaata gacctaaagc ccttaatgca ataaattcaa agactttaga
     4861 agaactttat gaagtatttg tagatattaa taatgatgaa actattgatg ttgtaatatt
     4921 gacaggggaa ggaaaggcat ttgtagctgg agcagatatt gcatacatga aagatttaga
     4981 tgctgtagct gctaaagatt ttagtatctt aggagcaaaa gcttttggag aaatagaaaa
     5041 tagtaaaaaa gtagtgatag ctgctgtaaa cggatttgct ttaggtggag gatgtgaact
     5101 tgcaatggca tgtgatataa gaattgcatc tgctaaagct aaatttggtc agccagaagt
     5161 aactcttgga ataactccag gatatggagg aactcaaagg cttacaagat tggttggaat
     5221 ggcaaaagca aaagaattaa tctttacagg tcaagttata aaagctgatg aagctgaaaa
     5281 aatagggcta gtaaatagag tcgttgagcc agacatttta atagaagaag ttgagaaatt
     5341 agctaagata atagctaaaa atgctcagct tgcagttaga tactctaaag aagcaataca
     5401 acttggtgct caaactgata taaatactgg aatagatata gaatctaatt tatttggtct
     5461 ttgtttttca actaaagacc aaaaagaagg aatgtcagct ttcgttgaaa agagagaagc
     5521 taactttata aaagggtaaa aatctaatta aaaatatatt tgctgttaac tagattaaat
     5581 agtattttaa tctagttaac agaatataaa tactaaaata acaggaggtt ataactatga
     5641 aattagctgt aataggtagt ggaactatgg gaagtggtat tgtacaaact tttgcaagtt
     5701 gtggacatga tgtatgttta aagagtagaa ctcaaggtgc tatagataaa tgtttagctt
     5761 tattagataa aaatttaact aagttagtta ctaagggaaa aatggatgaa gctacaaaag
     5821 cagaaatatt aagtcatgtt agttcaacta ctaactatga agatttaaaa gatatggatt
     5881 taataataga agcatctgta gaagacatga atataaagaa agatgttttc aagttactag
     5941 atgaattatg taaagaagat actatcttgg caacaaatac ttcatcatta tctataacag
     6001 aaatagcttc ttctactaag agaccagata aagttatagg aatgcatttc tttaatccag
     6061 ttcctatgat gaaattagtt gaagttataa gtggtcagtt aacatcaaaa gttacttttg
     6121 atacagtatt tgaattatct aagagtatca ataaagtacc agtagatgta tctgaatctc
     6181 ctggatttgt agtaaataga atacttatac ctatgataaa tgaagctgtt ggtatatatg
     6241 cagatggtgt tgcaagtaaa gaagaaatag atgaagctat gaaattagga gcaaaccatc
     6301 caatgggacc actagcatta ggtgatttaa ttggattaga tgttgtttta gctataatga
     6361 acgttttata tactgagttt ggagatacta aatatagacc tcatccactt ttagctaaaa
     6421 tggttagagc taatcaatta ggaagaaaaa ctaagatagg attctatgat tataataaat
     6481 aagaatttgg gaattaaaag tttaaataaa attgtttaaa aaacaatttc ggatatatga
     6541 aaaatctaat ttaatggggg taatgaatat gagagaagta gtaattgcca gtgcagctag
     6601 aacagcagta ggaagttttg gaggagcatt taaatcagtt tcagcggtag agttaggggt
     6661 aacagcagct aaagaagcta taaaaagagc taacataact ccagatatga tagatgaatc
     6721 tcttttaggg ggagtactta cagcaggtct tggacaaaat atagcaagac aaatagcatt
     6781 aggagcagga ataccagtag aaaaaccagc tatgactata aatatagttt gtggttctgg
     6841 attaagatct gtttcaatgg catctcaact tatagcatta ggtgatgctg atataatgtt
     6901 agttggtgga gctgaaaaca tgagtatgtc tccttattta gtaccaagtg cgagatatgg
     6961 tgcaagaatg ggtgatgctg cttttgttga ttcaatgata aaagatggat tatcagacat
     7021 atttaataac tatcacatgg gtattactgc tgaaaacata gcagagcaat ggaatataac
     7081 tagagaagaa caagatgaat tagctcttgc aagtcaaaat aaagctgaaa aagctcaagc
     7141 tgaaggaaaa tttgatgaag aaatagttcc tgttgttata aaaggaagaa aaggtgacac
     7201 tgtagtagat aaagatgaat atattaagcc tggcactaca atggagaaac ttgctaagtt
     7261 aagacctgca tttaaaaaag atggaacagt tactgctggt aatgcatcag gaataaatga
     7321 tggtgctgct atgctagtag taatggctaa agaaaaagct gaagaactag gaatagagcc
     7381 tcttgcaact atagtttctt atggaacagc tggtgttgac cctaaaataa tgggatatgg
     7441 accagttcca gcaactaaaa aagctttaga agctgctaat atgactattg aagatataga
     7501 tttagttgaa gctaatgagg catttgctgc ccaatctgta gctgtaataa gagacttaaa
     7561 tatagatatg aacaaagtta atgttaatgg tggagcaata gctataggac atccaatagg
     7621 atgctcagga gcaagaatac ttactacact tttatatgaa atgaagagaa gagatgctaa
     7681 aactggtctt gctacacttt gtataggtgg aggaatggga actactttaa tagttaagag
     7741 atagttttag attataatat ttacaaatta aatatttaat tatgaattta acctgtaaga
     7801 ataactattt gttattctta caggttttta tagttaaaaa taattattat ctattaaaca
     7861 aaaatagtgt aaaatatata atatcatgat tttgaagata taaaaaagta ggaagatata
     7921 aaagaaagta ggtaaaagtt tgtttaaaaa agagaatcaa agatataatc ttatatcgta
     7981 tacaaatgaa gaagaaatga ctttaaaaga agttttattg gacaagctaa atttctcagt
     8041 taggtcatta tcaaaaatga agagggaaaa gagtgtttta gtaaatggag tatataaaaa
     8101 accgagttta aaggtgtata gtggtgattt aatagaagta aaaatagacg aggaaaaagc
     8161 aaattttgaa cctcaagatt taaatttaca gataatttat gatgattttg atataattat
     8221 ggtgaataaa cctccgttta tggtagttca cccaacaaaa agccactatg ataaaacaat
     8281 agcaaatggt attagttact atatagataa tcaaaaagaa aatgtaaaaa taagatttgt
     8341 aaataggtta gatatgaata cttcagggtt agttatagta gcaaaaaatg cttatgcaca
     8401 tcatactttg tctacagcaa tgagtgaaaa taaagttgaa aaaaagtata taacagtagt
     8461 tgatggaatt ataaaagaaa atgagggaac tatagatgag ccaatttata gacctacaga
     8521 agattcaata aagaggataa tagatgaaag aggacagtca tctgttactc actataaagt
     8581 aatagaaaga ctagaaaatg caactgtatt agaagtaagc ttagaaactg gtagaacaca
     8641 tcaaataaga gtacatatgg ctcatatagg tcatggaata ataggtgatg agttgtatgg
     8701 ttatgtagat gaagatctaa taaataggca agcacttcat gcatataagc tagagtttga
     8761 acaaccaaga acaaaagaaa aattgaaatt taaagcagat ataccagagg atatgaaaga
     8821 gttaatatcg aaattaagat aatggggtga tatatatgat aatacataaa tttataatac
     8881 atgttttgga caaaaatagt gatgttccaa tattaaatga ctttgaaggc aaagttaatc
     8941 aagaagtaga tggttttttc cagaaagcta taaaaagaat tgcaaaagat gaggatttaa
     9001 gaaaaggtgt ttttaaagat tataatgaca atttaattaa aaactgttgt gaacaaataa
     9061 tatatgatga aagtactttt ttgaaaaatt ctaaagaaat tgcatcttac ttgtttgatg
     9121 ttatgaaaat taatgctgca ctagaatctt gtgatttggc aatttgctta tacactatta
     9181 aagatgaaaa gagtgtagca atactgaaat tagattataa aaagttgtac actcattcaa
     9241 ttgaatttgt ggatgataaa tttaatattc aaatggtttc aaatgaaatt ggtatcccag
     9301 agactttaag acaaaagcaa ggagctttga ttagtctaag tggtataaat gatgagtttc
     9361 atctaaggct tttagataag gatgctgaaa aagaagggtc agaatctaag tttgtaacag
     9421 aatttttaaa tgctaaaaaa atagatgatg ataagtacaa gaccaaggta tttaaaaata
     9481 cagctgaaaa ttggataacc aatgctctta gtaatgatat aaagcaagct gaagatgtaa
     9541 gaagtatatt aaattataca ctgaaggaaa aacatgaagt tgatataaat gactttgttg
     9601 ataattcaat taaagatgat gagttaaagg atagctttaa agagcatatg gaagaaaaag
     9661 gtcttgacga aagttttagt atagataaaa aatgggttga gaaaaagctt aaaaaaagaa
     9721 gtataaaaac ggataatggt tttgacataa aagggaatct aactgatttt gaagacccaa
     9781 tgaagtatac tgtaaaacaa aatcaagatg gaactataga tataataata aagaatgtta
     9841 cgttttatga agaaaagtaa tttatatact tagtatataa attaaatata agagcgtgta
     9901 ttttatatat tgtaaataaa tgtaatctag tctgtgcgaa ctctaatata atttattatg
     9961 tagaagttta cacagactat ttatatttgt agacaaaacg atactatttg ttttcttcga
    10021 tatatttaca tatatcacca atacatttaa agttttcagc ttcctcgtct ggtatttcta
    10081 ttccaaattc atcttcaagg gccattataa tttcaacagc atctaatgaa tcagcttcta
    10141 agtcgtccat taaagatgct tccatagtta tctcatctaa attgtctacc cctaattgtt
    10201 ctgctattat ttcttttatt ttctcaaaca ttttaagcct cctaaagcag ttttaattaa
    10261 ttttaatgtt agtttaatat aatatcaaga atttttcaat atggaatcta taaaaaataa
    10321 taaaatttta taaatgtagt aataaagtat tatttgatga ataaacttta ttgataacaa
    10381 atataactta ggaggaaatg ctatgaaata tgtaggacta ttactaactt cagtaggaat
    10441 gtttttactt atagcagtaa atttttatta taattcaata actttagata tgcaacgtat
    10501 agaggattat gtaatggaga ccaatttaat ccttgaagat gtagctgaaa aggagagtta
    10561 tgtatctaat gaaaaagaag attacatatc aaggttaatg catgtaaaga aaggaataga
    10621 aaattcaaaa acatccttct tgatagaaag atataaggag tacaaaataa aatcaataga
    10681 aagtttaatc tatactatat cagaggagaa aaaagattat ctggatgaag ttgatagata
    10741 caataaactt ggtgaaaaag aaataaataa actaatcaat aaaaactttt tagaagtaac
    10801 ttatctgtct attactacat atatttaatt aaagagacaa aacttagtct cttaatgatt
    10861 caaggaggat tagttatgac aaaattagaa gacaatatgg aacgtttctt tggaggatgc
    10921 gaaggatttt ttgggaataa aatgttgata ataatagtta tagtattctt actattatgc
    10981 acagacattt tagaagactt attatgcgat gacaatatat ggatatgggt tatattaata
    11041 gtattgttat tatttaactt cgatgatggt tgttgttgct aaaaaatatt gattattaaa
    11101 taggtaaaac cagtaactta tacaagttat ggttttacct tttttagttc aaattaaaaa
    11161 atttaaaaaa atagtaaaaa tacattagca aaatggaaac caaatgcata gtatatgtta
    11221 ggagcgaaaa gctcaacaaa atatcaacag gaggtaacgt ataatgttag atagattctt
    11281 tggagaagaa ggatgttttg gtggaggagg atggtggata atagtactat tcttcctatt
    11341 cttagctttt ggagatagct gggcagaatt tgacataatg gcatggatac cattcctaat
    11401 agttttatta atactttgct gctgtggtgg atgcttagaa taagaacaca gttattaaaa
    11461 aaaggagggc tttgttatgt tagatagatt ttttggagaa gaaggatgct ttggtggagg
    11521 aggatggtgg ataatagtac tattcttcct attcttagct tttgaagatt gctggagaga
    11581 cattgacata atgtcatgga tacctttctt aatattattg ctaataactt gttcatgtgg
    11641 tggattcttt gatggtgaag taggttgcgg ctgctaatac ttaaatcatt agaataaaaa
    11701 aatgctggta ttttatacca gcattttttt attttatttt ttaatatgat ttaaattaag
    11761 aatgaatatt attaaatttg ttgaatatag ggtataatat aaaatataat gtatttaaat
    11821 gtacttgctt ttatggtaaa atgaaaactt atataaatgt agttaaaata ggaaccttca
    11881 aaaatttggt tcagttttag ataaatttag taaaataagg aggatgaaga tgaaaggcaa
    11941 tataacgata aaagatgttg ctaaacaagc aggagtgtca atatctactg tatctagagt
    12001 tataaatgat tcaaaacctg taactgatga agtcaaacaa aaagttttag aggttataaa
    12061 agagactgga tatataccaa atccacttgc tagaagctta gtaacaaaga agagtcaatt
    12121 aataggggta atagttccag aagtttcaga ttcttttgtt aatgaggtgt taaatgggat
    12181 agaagaggtt gctaaaatgt atgactatga tattctttta gcgaatacat actctgataa
    12241 ggaacaagaa cttaagagta taaatctatt gagagcaaaa caagtggaag gtatagttat
    12301 gatttcatgg atagttgaac aagaacatat caactatata caaaattgtg gaataccagc
    12361 gacatatata agtaaaactg ctagaaatta tgatatatat acagtaagta ctagcaacga
    12421 agaagctact tttgatatga cagagcatct tataaagaaa ggtcatgaaa agatagcttt
    12481 tataatgact agtaaagatg atactgtttt agaaatggaa agacttgctg gttatgagaa
    12541 agcactttca aataacaata tagaattaga caagagtttg attaagtatg gtggaactga
    12601 ttatgagagt ggatacaata gtatgaaaga actattagat gatggaataa tacctcatgc
    12661 ggcttttgta acaggtgatg aggctgccat aggtgctata aatgctatat gtgatgctgg
    12721 atataaggtt ccagaagaca tatcagttgc aggatttaat gatgttaaga tagctagaat
    12781 gtatagacct aaacttacta cagtatatca acctctatac gatatgggag cagtagcaat
    12841 aagaatggtt ataaaattaa taaataagga attaattgaa aataagaaaa tagagttacc
    12901 ttatagaatt gttgatagag aaagtgttac agaaagaaaa aaataaaact attaaaatca
    12961 atcaggtagt gccttaggca ctacctgatt ttttatgtca ataatatcaa atttaataat
    13021 agattaacta aaaataatta tctaattgct taaatagttt atttttatct attcatgagg
    13081 attagacttt ttagttttct taaataaact tttagatttt ttttgttgac tacctggtgt
    13141 attttttggt gcaggtgaat ttgacttacc tttagtaggt ttagccttct tagttttagc
    13201 tggtttagta tctctttggt tttctgcatc tgagattttt tccattatat tatccagttt
    13261 tttgcgttga ttttcatcta aaaatgcttt gaattttgat actacttcat ctctgccatc
    13321 tagattctta cctattttta taagttcctc cataagttca ttttctgatt ttcctttgta
    13381 actatcagcc atcttttcta ttttatcctt gtcaatattt ttttgtttag ccattttttc
    13441 taatgactct gtatctaatt ttttcataaa aaaacccctt tctataaata tatattgaat
    13501 tatattcata ctatttatat atagaaataa tattgaagtg gtgaattgtg agtgatatat
    13561 aatataaata aaaattctta tagataatat atatgcactg agctttaaaa atggaacatt
    13621 aaaatatgaa aaacatactt taatataggg caattatata atataagggg gaattgtctt
    13681 gaataactta ctatttttga ttggagtgat tgcattgagt aatggtaata tctctatatt
    13741 tgatgataaa aataaatcaa aaggaaataa tagagttaga aaaaacaaaa aaaatgcaaa
    13801 tgataaactt tcacagtcta cacatggcaa tttaaggttt gatgcaaatg atataaaaag
    13861 aggtttaagg ctcatggatt taagtgaaga agatttagaa atgggaatgg aaataataac
    13921 aagaactaaa aaatacatgt caagagatga aagaaaaata ctaataaaaa tagaaagtgt
    13981 tttagattta gttagaggaa taaaaaaatt aaataatata gatatggtag atacagaaga
    14041 tgaaacggat tttttcagaa aaatggatag tgatgataaa aagaatatga tgataaagga
    14101 aattatagat gtattcccag aaaaaagaaa aaattcagtt gaaaaagcta taggaatgaa
    14161 aaagaagata gatttatttg cagaattatt tttaccagat gactttggtg aaggtggatt
    14221 tagtttgagc tctttggcaa atataaacaa tcttggtagt atgaataatc tcaaattgtt
    14281 aggaagtctt ttaagaggtg atgatagtaa taatgatgat gaagacaata atgatgagtt
    14341 aaattatgaa gacgaagaat tagattgtga agacaaagaa ttggatgatg atgagttaac
    14401 agatgaaggt gaaatggagg ataatgaaga tgaattagta gaagataaga acacaggcta
    14461 tgaaaatata gaaaatgatg aagatatagt atacgatgag gaactataca acattaatga
    14521 gcaagaatcc gaatacaatt ataatgaaaa aaatactaaa actagagcta agaaaaaacg
    14581 ataattaact aaatacataa agaaaaaagg aagaaaaagt aatgatagaa taagattatc
    14641 tcaattacta tataaaacca ctttaaaaaa ttcaaaattt attatataat aataatatga
    14701 aatcctgaaa catacgataa ggctttttta aattcaacct acaaatgaaa tacgggagac
    14761 cgtgtctggt aaattataac atgcctagtt agtttggacg ttaaaggtta ctggcaagtc
    14821 acccacctgg gagtgcctag ggtgtgaatt tacttagcca ccggtgtttc gggacaaaaa
    14881 aagtgctact atttcaaata gtagtacttt ttttattaat atgcattttt ctactaaata
    14941 cgcctaatta ttgataaatt caaaaagcaa gtaacaatta agattataaa agcatagtat
    15001 taattaggct tcaaaaagcc aaagacttaa taagaatcgc ttacagggag ggcaatgaaa
    15061 aatgcaagat tataaaaaaa ataaaagaag aatgatgaat cagccaatgt ctacaatgaa
    15121 tgaagaagaa gtgtatacag atgaaataaa ttcagaagac atgagaggtt ttaaaaaatc
    15181 acaccatcat aatggatgta atactgataa taagtgtgag tgccatgatg attgcaatcc
    15241 atgcaaccca tgtaatccat gtaaacctaa cccatgcaat ccatgcaaac ctaatccatg
    15301 tgatgacaat tgtggatgcc atgacaattg taaatgtgat tgtgaaccat gtgaaatgga
    15361 ttcagatgaa tgttttgaaa acaaatgtgg accagaatgc tgtaatccta tatctccaag
    15421 aaacttctct gtatcaaatg cagtgccatt tgcaatagag gctaatagaa tatttgatac
    15481 tatgcaattc caaacattta cagatgcaac aggaccaaat ggagagccat taacttttga
    15541 aacagaagta gtagaagtat ttggttcagt tccaagtgca ggtcaagcaa gtgtaactat
    15601 agaaaaaata tgcttaagta atgatggaat cgttatagac acaggaatga caactttaga
    15661 agatttcgat ttagacccat taggagatat agtaggaaga aactgtgaaa caacttttga
    15721 atttgcagtt tgtggagaaa gaaactctga gtgctgtaga caaggaaaag gcaaatcagt
    15781 agcttataaa caaagaggat taactgtagc agttcgtaat ttagtactag agctaagagg
    15841 tagatgtgga tgtacagagt tcgttgcatt agctttccca gcagttagag caggaggtgg
    15901 atgtaagaga agagttgatt atgtagaatt tacttttaac acactttcag caccaatatg
    15961 cttgccagct gacggaagag ctgttacttt aagacaagaa tatcaaacta acttaactgt
    16021 agattgtata ggaaaatcta tattaaaatt agaatgcaac gaatgttgtg aacctttcta
    16081 tgaattaatt ataccaaatg atatagattt agtactttgc ttacaagaaa cagttagcac
    16141 attaataagt gaacaaatag tagttttagc atcaccaaat ccaatccaac caagacttgt
    16201 tgatactttc tctaaagtat gtgatttttc gcaatgtgga cctaatcatg gaagtggaaa
    16261 gccaagttgc cacagataga taggtagata tatagacagt ttaaattata aataaaaatc
    16321 atctaaggaa ggctaccaaa gtgtataatt tacattggta gccttcacgt ttttataact
    16381 agcgtataga ttttagtgat ttaaaaagca ctatagtatg taatagacaa gtaaagattg
    16441 atactatcgt cattgtgtat atgtatgcat aaatattaag tcctggaatt ggaagaaata
    16501 catataaagc tattagttga attaacatgc cagtaatagt atttatactt gaagctaatt
    16561 cttttcttat tgaatgtaaa atgccagata gagtttgatt tagggacata aatagtggaa
    16621 ctaaaaacat agcttttaga tattctcctg caagtgtatt tttaaatact aatatacata
    16681 aatcttttcc aaagaaataa aaaaatattg atgatagtat gcctacaaat aaagtaagaa
    16741 gtatggagta tttgattttt ttaattacac tcttacgctt tcttagagcc atttcttgtg
    16801 aaatagttgg tattaaattt acaataagcg ctgaaccaac agtaaaaggt aaaaatataa
    16861 aaggcattac catcccactg acaataccat acatacttaa agattgttga taagtcattc
    16921 ctgataatgc taatcttgat ggtatcatca tagaacttat agattgcaaa agtatgtttg
    16981 acattctatt acaagttatg ggaatagaca tttttaatgt ttccatagaa gaattataaa
    17041 aatcttttat gcttataata tacctattgt caaatgatga atctcgccat aagcaaataa
    17101 gcataaatat aatattactc aactcaccaa tcgatatacc taatagagct atataacaat
    17161 tcatagattt attatttatg tacataacaa gtaaaaaaac aaataatatt ttaccaattt
    17221 gttcaatgat ttgtccaata gcagggattt ttacattctt tatgccatag taatatcctc
    17281 ttagtacgtt tgaaagagtt attataacta tagcaggaca aacagctagt ataaatagat
    17341 ttaatttagc atcttttaaa aatttaagtg ataaaaaaga actattgaaa gatgcaacta
    17401 tagatataat tagtgctacg aaaaatgata tatataaagt tgatataaat aaagcattag
    17461 tattgtgtct atcgtttaaa gcttttctct ttgcaactag acaactaagt gcagtaggta
    17521 tacctgttgt tgtaacagct aagcaaatca tcaaaaagtt aaaaatcata tgatagatac
    17581 ctaaacctgt ttcacctagt gctcttgata agaatatttt atataaaaaa ccaaatattc
    17641 ttacaataaa attggatata aataaaataa ctgttgatag tactaaataa ggtactttca
    17701 aaaaaatcac ctccttatct tttaaatata ttcacaagga ggtgtaaaaa tttataattt
    17761 tataaattct ggtttcaatt tattaaattt agatacatat ttaaatccca tattttttag
    17821 taatgaatat atgtagtcaa actcacatgc aacttgactt atatgatgtg aatctgaacc
    17881 tgtggttatg atttcccctc ccaagtcttt gtacatttga agtatatact tagatggggt
    17941 taggtctgga agattatatc tataacaaga ggtatttatc tcgatacctt tgccatcata
    18001 aatagcttgt ttaagagttt cttcaattat atcagaaaat agtctatcat ctaaaatagt
    18061 gtcataaggt gcataccttt taatcaaatc taaatgacct aagacagagt aatctttata
    18121 attcttaaca atattatata aataaagata gtaattttca taaacttcat gttgagtttt
    18181 atctttaaaa taattaccaa ggtataagtc cattgtatct atagcatgga tagaacatat
    18241 tataaagtcc aatggatatt gatgtgcttc ctttttgtat gtatccatta attgagtttg
    18301 aactccaaat tcaattcctt tttttattga tattttatct ttatatttgc tttgaagagt
    18361 ctctaaactc ttaaaataat cttcgtagac aattgaaaaa gaatcatcag catatacatc
    18421 atagtcaaca tggtctgtga aacaaatttc ctccaaaccg agttcaatag atttttttac
    18481 catttcttcc attgtagatt ttccgtctgt agagaaactt gagtgcatgt gatagtcgta
    18541 aaacataaat atttcccctt tttataaaaa ttttggtatt atatatacat actaaataat
    18601 atcaaatata cataaaagca tcaataaaat ataaatacaa tgagcggtga taaatttgga
    18661 aaaatacaga atagatttaa atgagaaaaa atcaactaaa tatatacaga tatttaatca
    18721 tataaaagaa ttgataataa ataaagagtt agtagaacat gaaaaattgc ctccaataag
    18781 aaaactatca aattttttac aagtaaataa cactactata gttaaggtat acgagctttt
    18841 agaaaatgaa ggatatgtat ataaaattat agggagtggt acttttgtat caagtttaaa
    18901 ttcttataaa agcggtatag ataacaggaa aaataagatt attgtgcaaa gagaagattt
    18961 aattcatttt gacaatggga atccatctaa tgatatgttt cctatcgatt catttaaaaa
    19021 tgctgtaaat atggcattat ctaaagatgg tagttcaata tttgaatatg atgaaggtct
    19081 tggctctgaa gaattaagag aaaaaatggt agagtatcta agtgatggaa atataaaaac
    19141 tactaaggaa aatattcaaa taatatcagg tgctcaacaa ggtatagata tagtttgtaa
    19201 aggacttata agttattcag atgttgtttt tatggaagaa ccttcttaca atggagcaat
    19261 tgaagtgttt aaaagtagag gtgcaaaaat aatatctata cctatgttag atgatggaat
    19321 tgatataggt attttaaaat taaagttgga aaaaataaaa ccaagactta tatacattat
    19381 gcctaatttt caaaatccaa ctggaatatc ttactcaaca tataaaaaaa agaaattgat
    19441 agaattggca gaagaacatg atttttatat aatagaagat gattttataa gtgattttgt
    19501 ttttgaatca aaggacaata gaacaattag aagttatgat gataaaaata gagtagtata
    19561 tataaaaagc ttttcaaaaa tacttatgcc aggtcttagg ataggaattg tagaaatgcc
    19621 aaatgagttg ttaaaaaaag ttttatgggc taaatattca tcagatatat caacaccagg
    19681 tttaattcaa aaatcaatgt attactatat gaataatttt gattggaaga attatttgag
    19741 tcatatagag aagatatata ctgataagta taaattggca aaaaacttga ttaatgacaa
    19801 attgagtggt aaattaaaag ttagaaaatc ttgtggagga ataaactttt ttttagaatt
    19861 accaagagga tatacatctc aagcatttac aaattttatg ttaaacaaag gagtgtcaat
    19921 gcttccaggg acttattttt ttgacaatct agtggatgat agattcttta ggataaatat
    19981 agctcgtcca agcatggaag aactagaaaa gggaatatct ataataagtg ataatataga
    20041 tgagtttttt tgtgaatata aaaacaagtt agatataaaa agcaataaat tattttatta
    20101 aataaataga ggttttttag gaggaaaaaa tgtttgacaa gaagaaatta gataggatta
    20161 atgaattagc aaagaaaaat aaagagggaa tcctttctgc tgatgaaatc aaagaaagag
    20221 aaattttaag gaaagaatat ttagaaaact ttagagccca ctttagatca agactagata
    20281 gtgtcaaagt agtttcacca gaagaatatg agcaatacat gaaaaacaat aaaaattaga
    20341 acttagtagg aggaaaatat gaacttaaag tacgaattta agaatgcttg ggattttgaa
    20401 agaaaagaaa atactattga acaaattatg caatattcta gcaattatat ggagttttta
    20461 agtaagtcaa agactgaaag actttcagta aaagaaatta taaaattggc taaagaaaat
    20521 aactatattt caatagatga agctatggaa aaaggaagca taagttgtgg tgataaaatt
    20581 tatgttataa ataaagagaa ggcagtagca ctttttgtaa taggtaaaaa ttatatagaa
    20641 aaaggtatga agattatagg aagccatata gattcgccaa gattagattt aaaaccaaat
    20701 ccactgtatc aagagagtaa tctaggtttt tttaagactc attattatgg tggaataaaa
    20761 aaataccaat ggacggctat tccacttgct ttacatggta tagttatatt aaatgatgga
    20821 acaaaagtgg acatatctat tggggaagaa gatagtgacc cagtattttg tgtaactgat
    20881 ttattgattc atttggctgg agaccaaatg caaaagaaac tatcagaagg aattagtgga
    20941 gaagctttaa atatattaat tgggaatatg cctttagaag atgaagaaaa agaacctata
    21001 acagcaaata tattaaagat tttaaatgag aaatacaata tagttgaaga agatttatta
    21061 agtgctgaaa ttgaagtagt tccagctggt aaggctagag atttagggct tgataggtct
    21121 atggtgttgg gatatgggca tgatgatagg gtatgttcat atgctgctgt aaaagctata
    21181 ctagaaactg aacaaccaga atttacttca gttgcattat gtgtagataa agaagaaatt
    21241 ggttctaaag ggaatacagg tatgcactca aagttttttg aaaatactgt ggctgaactg
    21301 attgctttag aaggagatta ttgtgatatt aaagtaagaa gagctttagc aaatagtaag
    21361 gtattatcag ctgatgtttc tgctggatat gacccaaatt ttggagaagc atatgaaaag
    21421 agaaattcgg catatatggg aaatggagta gttttaacta agtatacagg ttcaagagga
    21481 aagagtggat gtaatgatgc taatgctgaa tttatgtcag aagtaaagag aatttttaat
    21541 aaaggaaatg tagtatggca aactgctgaa cttggaaaag tagaccaagg aggaggcgga
    21601 actatagccc atatattggc aaatcaaggt gcagaggtta tagactgtgg tgttggagtt
    21661 ttaaatatgc atgcaccaca tgagattgtc tctaaagtag acatatatga aatgtataag
    21721 ggatataagg cattttttaa tataaattta taagtaaaaa tataaatgta ataagtgaat
    21781 aaaggctgtt gtataaacaa tttaatctgt tgtacaacag tcttattttt atgtaaaaat
    21841 agcaatttgt cacactagag aaaataaaag aaaatactgt caaattaaaa attattttaa
    21901 aaaaaattga caacgatttc atcttgttat aatatgtaat aaagaacttg ttatataatg
    21961 ttataaaaat gttacatagg gaggtgagca aaatggatga aaagagacaa aaaaaagaaa
    22021 tggatttaga agtaaattcc tttgttcctt tatatcaaca actatatgac aacataaaaa
    22081 aacaaatagc atctggcata tataaaccag gagataaact tccatctgaa ggagacttat
    22141 gtaaagaatt taatataagt cgtataactg tgagaaatgc acttaatgaa cttgtaaaag
    22201 aagatatatt atgtaaaaaa cgtggtaaag ggacttatgt aacaatacca gagagaatag
    22261 aagcaacatg tgcaggaaat agttttacta attcttgtca tcgtattaat gcaaaaccaa
    22321 gtaccaaaat aatctctgtt ttaataaaaa aagcagataa gcaagtagct gaagctttaa
    22381 gtattgaact agaagagaag gtaatatgta ttaaaagact tagattaata gatgaagtac
    22441 ctgttatttt tgaagttgac tattttagaa tagattacat gtttttatta aaagaagaat
    22501 tagaagataa atctttaatg gaggttatat ctaataatat aagcacatta cctaaacgag
    22561 tagaaaatat atttgaagta aaacattcaa acaaagaata ttctgaccat cttaaatgtg
    22621 caagtaatat gccactattg aaagtaagac aatcggtgta tacagaaaat aatgatgtct
    22681 tatattacaa tgagcaattt ataagaagtg ataagtacaa atatgcagta tcagcagaaa
    22741 tataatctat aatttagtag gaggatgagt atgaaaaaga taatattagc atctcatgga
    22801 gacttttcaa aagggctttt aaatgcagta aaaatgatag taggagattt agcagattgt
    22861 gtcagttcgt atggtttgta ccctggacaa agtgcttcag attttgcttt agaattagaa
    22921 aaaacaatat cagaagataa agaatgtgaa tatataattt tatctgactt atatggagca
    22981 agtgtatgta cagcaatgtt aagattaact aatctacata atgttaggtt attttctgga
    23041 atgaatttga atatggtatt agaacttctt acaaggtttt ctgatgaatt aaccgttgaa
    23101 gatatagacc aattagtgga agaatctaga agaggaatac aaagcgtgac attacaaatt
    23161 aaagaagaag aagaggtatt ttagtttatg aaaaaaaagg gaatattatc agtatttaaa
    23221 agtaaaaaac caattatagc aatgatacac ttaaaaggag atactccaga agatatcttt
    23281 gaaagagcta aaaaagagat tacaatcttt gaagaaaatg gtgtagatgg aataatgtta
    23341 gaaaattact atggtaatta ttatgattta gaaagaatac ttgaatatgt atcaaaagct
    23401 aatttatcaa taccatatgg agtaaactgt ttaaatgttg atactatggg atttgaactg
    23461 gcaacaaaat ataatgctag ttatatacag gttgattctg tagtaggtca tgttaaacct
    23521 agagatgaag ctactttaga agagtttttt aaattacaac gttcaaaatg cccagcgtat
    23581 ttaattggag gcgtacgttt taaatatcag ccagtattat cagaaaatga tgttgaagaa
    23641 gatttaaaaa taggaatgac aagatgtgat gctatagcag ttacagaaaa tgctacaggt
    23701 caagaaacat caatggaaaa aatagaactg tttcgtaaaa atttaggaga cttcccattg
    23761 gttatagctg ctggagttac attagaaaat gcaaaaaaac agcttaaatt aggagatatg
    23821 gcaataatag gaagttactt taaagataat tacaaagatt ttggggatgt tagtgtagaa
    23881 cacgtaaaaa cattcatgga tgaaatcaaa aaaattaggg aggaattata atgattaaat
    23941 tagtaagagt agaccacaga cttatacatg gacaagtagc atttacatgg acaaagtttt
    24001 taagcacaga ctgtatattg atagcaagtg atgacttatt aaaagatgaa ttgagaatgg
    24061 caggacttaa aatggctaaa ccatctaatg ttaagttagt aatgaaaagc atagcagatt
    24121 ctataaaagc acttaactca ggtgttactg ataaatacaa cttattgata ctttgtgaat
    24181 ctgtagaaga tgtttataga cttgctaaag aggtaaaagc tattaaatct ataaaccttg
    24241 gtggaacaaa atcagatgat aatcgtgaaa atatatctaa agcagtgcat gtatcaaaag
    24301 atgatataaa gatgattaaa gagttagatt cagaaggcgt aaatgtattt gtacaattag
    24361 ttcctgatga tgatgctaca aatgtaatga aattaatata attttagggg gatgaaaatg
    24421 gaatttacac aagttatttt gataactcta attgcattct ttgcatatat gcatagtttt
    24481 gtaggttcta caatgcataa tagacctata gttgttgcac cacttgttgg tttagcacta
    24541 ggtaatcttc atacaggtat agtaataggt tcaacattag aattagtatt tatgggtgca
    24601 tttcctgttg gggcaagtaa tcctcctgat tttgtgtctg gttctattat tgccacagcg
    24661 tttgtaatat taacgggtca agatgtatca gcagcagttg tacttgcagt tcctatagca
    24721 actctagtat tgcttataga taacttctta atgacagtag tacttacatg gggagctcat
    24781 attgctgacc gttatgcaga agaggggaat attgaaggcg tagaacgtgt gcaattatta
    24841 tttggtatag gaaataaatt aatattagca ataatagtag gtataggatt ttcattaggt
    24901 gttccagtaa tagaaaaaat actttcattt ataccatcat atgtaactca tggaatggat
    24961 gttgcagcag gcgtgattcc agcaataggt tttgccatgt tagctagaat gatgcttaat
    25021 aagaaaacag tagcattttt attattagga tttatattgg ttgcttacct aaacataaca
    25081 gtaacaggag ttgcattgtt tggattagca atagctttaa tatatgttaa ctttgtagga
    25141 gagaaggagg taatagtaga tgacaacgag ttctaagaag ttagagacaa tttcaccaga
    25201 tagtaaaata acgaggaaag acttttggaa atgttttaga aggtcattga cattagattc
    25261 atcttggaac tacgaacgta tgcaaaatat agcatatgca tacatgatgg caccaataat
    25321 ccgtagatta tataaagatg ataaagaaaa gaaatctaag gctttaaaaa gacatttaga
    25381 atttatgtca gttactcctc atataagtac tcttttggta ggtatatctg gagctatgga
    25441 agaagaaaat gcaaagaata aagaatttga tgctaatagt ataaatgctg ttaaatcaag
    25501 tttaatgggt ccagtttctg ggataggaga ttcatttttc tggggaacat taaagcttat
    25561 agcagctggg gttggtatag ctttagcttc tcagggtaac ataatgggac cgattttatt
    25621 ccttttaata ataaatgttc ctcattttat tatacgctat atatgtctag ataaaggatt
    25681 taagtatgga actcaattct ttaaagatgt gagtggttca agtatagttt caaaagttat
    25741 ggaagcagct tcaatgttag ggcttatggt aattggaggt atgactgcat ctaatgtaat
    25801 gttaaaactt agtgttaatg taggtagtgg agaatgggca gagcctattc aaacttactt
    25861 agaccaaata atgccatgta tgcttccagc tatgatattc ggtattatgt attggttatt
    25921 aggaaaaaag gtaaagacaa ctacgatatt aatttcagtt atgattattt gtatagtatt
    25981 ggctgctatt ggagtagtat aaataaaaaa atcccaagtt catttgaacg agggattttt
    26041 tgactaaatt aaataaaaaa gaaagatggt tcataataat ttataagcat ttatgtgata
    26101 gttataatta gcttattaat tagttataaa ctcaaaatta agcactactt tattaaagtt
    26161 attatattat aatttcgaaa tactcacata atctgtttag aaatttatct tctaatgggg
    26221 aaaaagtttt ctttttagag taaatgaaga aaaactttct agtaaaatca acatccttta
    26281 ttttataaca ttttatttta cctgcattga gataatcgat agctgaaatg tacgatataa
    26341 aggacactcc aagacctaat ctaaccattt ccttaatagc ctcatttgat tctacatgag
    26401 cgattacatt taatttactt actggaaagt tatttttact aagtgtgttt agtattaagt
    26461 ttcttgtacc tgacccttct tttctcataa taaaatttag ataagccaat tctcctatat
    26521 cgatacaatt atttttatta tcaattttaa aatctgaagg agtgataaga acaagttcat
    26581 catctaataa gtctaggtac tctatttgag gattattgat tttagaacca actaatccaa
    26641 agcttattcg ttcatttaaa atttctgata tagcatattg agaatcataa tggcttatgg
    26701 aaaattttac atctggataa ctcatagaaa aactcttcat aaaatcaggt aaaatataag
    26761 tttcaggtat ggaactacat gctatatcta taataccttc tatttttcct gaatattctt
    26821 ttatatcata aattgcacgt ttgcaattat tcaatatata tatagcatgt tcataaagaa
    26881 tttcaccaga ttttgtgagt gtaataactt tgttgcttct atttataaga acagtttcta
    26941 gctctctctc taaattctgt atgtgtgcgc ttacagtagg ttgagttaaa aaaagttctc
    27001 ttgcagcctt tgaaaaactc tgatgttttg caacagcaac aaatacttca agttgtttaa
    27061 aatccattat ttaatcctcc ttaaattatc taaaactatt ataacatatt tgataaatta
    27121 atagtcaaag gtgattttta aaatagaaaa cctttaaata ttcatggagt ttttgattgt
    27181 ttaggaataa tttggtataa tgttgtaagg tacttacaat aaaaatatcg taaattgaat
    27241 tttcaaaatt caactttaaa gtgtttttta ttacagaaaa attacaatta gcttct
//
LOCUS       DACULQ010000029        26990 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.029, whole genome shotgun sequence.
ACCESSION   DACULQ010000029 DACULQ010000000
VERSION     DACULQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.029"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            49..1869
                     /locus_tag="JA154_05410"
     CDS             49..1869
                     /locus_tag="JA154_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT7995296.1"
                     /translation="MTLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPEYLNVWELQ
                     GITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLRKTSAPITE
                     LTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPILLVEKNNV
                     PNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRYETSLLIAK
                     EIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTYKWLKSEDL
                     QNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFYTDDELEAV
                     LVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVYKIGGGLTS
                     KVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIKEKDYTLNT
                     SLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNASDTTGNGVE
                     VFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLYVLRNNDMP
                     AVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            2377..4389
                     /locus_tag="JA154_05415"
     CDS             2377..4389
                     /locus_tag="JA154_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT7995297.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKIYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            4631..6544
                     /locus_tag="JA154_05420"
     CDS             4631..6544
                     /locus_tag="JA154_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995298.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKVTTYISFVKKELVKYWPLIIEKCDGISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            6537..8756
                     /locus_tag="JA154_05425"
     CDS             6537..8756
                     /locus_tag="JA154_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAT7995299.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKSGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLSLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            8865..9695
                     /locus_tag="JA154_05430"
     CDS             8865..9695
                     /locus_tag="JA154_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HAT7995300.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            9685..13152
                     /locus_tag="JA154_05435"
     CDS             9685..13152
                     /locus_tag="JA154_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HAT7995301.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNQTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIINFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13152..16979
                     /locus_tag="JA154_05440"
     CDS             13152..16979
                     /locus_tag="JA154_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAT7995302.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKSDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17025..18236
                     /locus_tag="JA154_05445"
     CDS             17025..18236
                     /locus_tag="JA154_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAT7995303.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18223..21750
                     /locus_tag="JA154_05450"
     CDS             18223..21750
                     /locus_tag="JA154_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease SbcCD subunit C"
                     /protein_id="HAT7995304.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEIPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            21881..23134
                     /locus_tag="JA154_05455"
     CDS             21881..23134
                     /locus_tag="JA154_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HAT7995305.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            23283..24335
                     /locus_tag="JA154_05460"
     CDS             23283..24335
                     /locus_tag="JA154_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HAT7995306.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            24425..25651
                     /locus_tag="JA154_05465"
     CDS             24425..25651
                     /locus_tag="JA154_05465"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087527.1"
                     /note="catalyzes the release of the N-terminal amino acid
                     from a tripeptide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAT7995307.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPKIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            25858..26910
                     /locus_tag="JA154_05470"
     CDS             25858..26910
                     /locus_tag="JA154_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HAT7995308.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADNISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT        11501 a         2645 c         4783 g         8061 t
ORIGIN      
        1 acctcaaaag tcagtgttaa gtttaaagaa aaaccagatg cagatagcat tacattaaag
       61 tataaatgct atgatatgcc attggataca actttaaact acaatcaatc aactgagtca
      121 tatgaaggaa ctatcaatta taataaagac ccagaatatc taaatgtttg ggaactacaa
      181 gggataacaa taaacagcaa aaataatcct aaaactttaa acaaacaaga actagaaaag
      241 atgggattaa atttaaaaga ctataatgta acacaggaat gtataattga agatataact
      301 tctagaaaag atgtaaataa atatttaaga aaaacttctg cacctattac agaacttaca
      361 ggaagtgata gatacgaaac agcagttaaa ataagtaaag aaggctggaa aaatggttca
      421 gataaggtag ttataataaa tggggatgta agtatagatg gcattatatc aactccacta
      481 gcaactacat ataatgcgcc aatacttttg gttgaaaaaa acaatgtacc taatagtgta
      541 aaatcagaat taaagcgttt aaatcctagg gatgtaatta taattggaga tgagaatgct
      601 atttctaaaa ctactgctaa tcaaattaaa tcgactgtaa atgctagtca aacacgtcta
      661 aagggctcta atagatatga gacatcttta ttgatagcaa aggaaataga taaaaatcat
      721 gatgtggaaa aagtgtacat aacaaatgct aatggcggag aagtggatgc acttactata
      781 gcagcaaaag caggtcaaga caagcaacca attatattaa ctgataaaaa tagtattaca
      841 gacaacacat ataaatggtt aaagagtgag gatttacaaa atgcttattt tataggtggc
      901 cctcaaatga tatcaacaaa tgttataaat aaggtaaatg atataactaa agataatgtt
      961 actaataata gagtatatgg agcagataga cacgaaacaa atgcaaacgt aataaaaaaa
     1021 ttctatacag atgatgagct agaggctgtt ttagtagcta aatcagatgt acttgttgat
     1081 gctttagcag caggtccatt ggctgccaac ttaaaatctc caatacttat aacaccaaag
     1141 acgtatgtat ctgcatacca taaagaaaac ttagaagcta aatcggctaa taaggtatat
     1201 aaaataggag gaggattgac ttctaaggta atgaactcta tagcatcatc attatctaaa
     1261 cacaatacga ctccaacaga accaggaaat agtgggggta agacagttat gattgaccca
     1321 ggacatggtg gttcggacac tggaacaaca ggtaaaccat tagggggtat taaagaaaaa
     1381 gactatacat taaatacttc acttgcgaca actgaatatt tacgttcaaa gggattcaat
     1441 gtaataatga caagagatac agataagact ttatctcttg gaaatagaac tgctttatct
     1501 aactctttga gaccagattt atttacgagt atacactata atgcatctga tacaactggg
     1561 aatggtgtag aagtatttta taagcttaaa gataaagatg gaggaactac taagactgta
     1621 gctacaaata tattaaatag aatcttagag aaatttaatc taaaaaatag aggtgcaaaa
     1681 acaagaacac tatcaactga ccctacaaaa gattatttat atgttttaag aaataatgat
     1741 atgccagctg tacttgtaga atgtgcattt ttggataatg agaaagatat gagtttactt
     1801 aatacatcaa ataaagtaaa agaaatgggt acacaaatag gtaaaggcat agaagattca
     1861 ttaaaatagt ctacaatgag agcatagaaa aatttaaaat aaattaatct ataattttaa
     1921 atttaaacta aagctataag ataagaatgt aattataata gtaataaaga ctgtatatac
     1981 attgtgcata taaatgcgaa atgaggtata tacagtcttt ttataattaa ttgtaaaatg
     2041 atttatgtta tatgaaaaat ttacatatat taaggctaca gttaattgtc aagagctgga
     2101 gtattcattt gtaatacaca atataagctt aattactata ttttattgtg tgttttttgt
     2161 ggatttttat gtcaataaat gatatagtat agataattaa atgtattaaa tatatttaat
     2221 ttttttattt tttgagtttt ataagtttat ttttatatat tacttatata ttacttatat
     2281 attatttata ttttgggtta gatacacttt gacatatttt caaaagttaa aagattaaaa
     2341 ataaagtaga aatttatgtt aagtaaggag attaatatga gaagaaatac aaaattatta
     2401 acaacaggga ttctttcaat ggcaatcgtc gcacctacaa tggcatttgc tactgaatct
     2461 aatgctatgg aaaataacgc tgatttaaat ataaacttag agaaaaaaag tatcgtttta
     2521 ggtagcaaat caaaagttag tgtcaaattt aaagaaaaac cagatgcaga tagcattaca
     2581 ttaaagtata aatgctatga catgccattg gatacaactc taaattacaa tcaatcaact
     2641 ggggcatatg aaggaactat caattataac caagacccag aatatctaaa tgtttgggaa
     2701 ctacaaggga taacaataaa cagcaaaaat aatcctaaaa ctttaaacag acaagaccta
     2761 gaaaagctgg gattaaattt aaaagactat aatgtaacac aggaatgtat aattgaagac
     2821 ataacttcta gaaaagatgt aaataaatat ttgagaaaaa cttcttcacc tattacagaa
     2881 cttacaggaa gtgatagata tgaaacagca gttaaaataa gtaaagaggg ttggaaaaat
     2941 ggttcagata aagtagttat aataaatggg gatgtaagta tagatggcat tatatcaact
     3001 ccactggcaa ccacatataa tgctccaata cttttggttg aaaaaaacaa tgtgcctaat
     3061 agtgtaaaat cagaattaaa gcgcctaaac cctaaagata taattataat tggagatgag
     3121 aatgctattt ctaaaactac tgctaatcaa attaaatcaa ctgtaaatgc tagtcaaaca
     3181 cgtttaaatg gttctaatag atatgagaca tctttattga tagcaaagga aatagataaa
     3241 aatcatgatg tggaaaaaat atacataaca aatgctaatg gcggagaagt ggatgcactt
     3301 actatagcag caaaagcagg tcaagacaag caaccaatta tattaactga taaagatagt
     3361 attacagaca atacatataa atggttaaag agtgaggatt tacaaaatgc ttattttata
     3421 ggtggtcctc aaatgatatc aacaaatgtt ataaataagg taaatggaat aactaaggat
     3481 agtgttacta ataatagagt atacggagca gatagacacg aaacaaatgc aaacgtaata
     3541 aaaaaattct atacagatga tgagttagag gctgttttag tagctaaatc agatgtactt
     3601 gttgatgctt tagcagcagg tccattggct gcgaacttaa aatctccaat acttataaca
     3661 ccaaagacgt atgtatctgc ataccataaa gataatttag aagctaaatc agctaataaa
     3721 gtatacaaaa taggaggagg attgacttct aaggtaatga gctctatagc atcatcatta
     3781 tctaaacaca atacgactcc aacagaacca ggaaatagtg ggggcaagac agttatgatt
     3841 gacccagggc atggtggttc agcacctgga aattcatctg gaggaatgat tgaaaaagat
     3901 tacaatttaa atacttcact tgcaacaact gaatatttac gttcaaaggg attcaatgta
     3961 ataatgacaa gagacacaga taagacttta tctcttggaa atagaactgc tctatctaat
     4021 tcattgaaac cagatttatt tacaagtata cattataatg gctcaactaa taaacaaggt
     4081 catggtgtag aagtatttta taagcttaaa gataaaaatg gagggactac taaaactgta
     4141 gctaccaata tattaaatag aattttagag aaatttaaac ttacaaatag aggtataaaa
     4201 acaagagtac ttcctagtga ttctacaaaa gattatttat acgttttaag aagtaatgat
     4261 atgccagctg tacttgtaga atgtgcattt ttggataatg aaaatgatat gagtttaata
     4321 aactcatctg caaaagtaaa agaaatgggt acacaaatag gtaaaggaat agaagattca
     4381 ttaaaataaa tggaaataaa aatatttaaa aacttaatgt aaatttatag ttgtaaattt
     4441 atactaaagt caaaaaatga aatataagtg tatcaatagt aagactgtat gtacatttgc
     4501 acatgtgaat gcaagtatat atacagtctt tttttgtagt taaatgcaat aatttgattg
     4561 tgttttgtaa aatacacaat atgatataat tagataatat acacttaatt taattgagga
     4621 aggtgattat atggaatatt tatttgaaaa aagtagttta aaagaatatg gaaatgaagc
     4681 tttagataaa tatagagaat tattagataa aactggtaat aattcaaggt gtattctttt
     4741 gttagtacca aacaataaca caaagatacg atatgaaagg gctctaagat taaattatag
     4801 tgaagaactg aaagtaacaa catatataag ttttgtgaag aaagaattag ttaaatactg
     4861 gccactaata atagaaaaat gtgatggaat aagtacaaag gttgtatctc caacatttat
     4921 atcaaatagt ttaagtgaat atttaatagt gcaaaaagtt aaggaaaaaa ggatacaaga
     4981 aggatatttt gaagatatta caggaaccaa taaatccata gcaaatagta taatgactaa
     5041 cataaataag tctgcattta atttaattga ttttgatttt attggagaaa aaatatattc
     5101 atcaaagaaa aataaagata gtatatatag attttcatac actcaaatgg atgaaattat
     5161 atcatattat ataaattcct tactatcaaa ttcaatttta gataatgcat taagcatata
     5221 tttatacaat caatacctac ttaatgatga tttttattta aaaaacttat ttacagaagt
     5281 gagatatata attgtggata gccttgaaag ctcaagtaat gcagaagttg attttataac
     5341 agaagcattg gataatacat tacaaagtta tatatatttt gattatagta gggattattc
     5401 tgtatttaat aatgtggata tagactatat aaatgagaaa ataatatcaa aaattaagcc
     5461 taaagaagaa aatattaaaa agaaggagat tagaatagaa gatttatact tattacctgc
     5521 aaatatagaa ttaaatcaat caagtcaatt atataatgaa atgttagatt taataagtga
     5581 aaaagttata tctcttatag aagaagatgt atctccaaga gatatagcaa ttatatctcc
     5641 tataaacaat agtatattgg agcatcaaat tagagattct ttgatagaaa aaaatataga
     5701 tgtttttagt actaagaagg ataaaaaagc tgtggattat ccatatggga atgcattggt
     5761 agtggctaca tgtatattct atggatattt agattttata aaagatgatg aatacttaag
     5821 ctttttagaa actcttttag aaataaatag gataaaagca tttaagattt ttaatgaaac
     5881 aagacattta agtattgata atatagatga acatagtcag tatcgtgata ttttacagta
     5941 tattgaagaa aaaaagaaga gtgatataaa aatacatgaa tttttaactc aattttacat
     6001 agataaaatg ttaaatttaa aagaaggtaa aaagaatgtt gggttatgta aaaagataat
     6061 agctgaaagt gagtctttta gtgaaagttt aagtctactt gggatgaaaa aagaaaagat
     6121 atttgtagaa gctttaaaaa gtacaataaa cgattattac tctgttgtcg atatagaaga
     6181 gttgaaatcc aaagacaaaa tagtaataac tactccttat tcatatatat catcaaatat
     6241 agatagaagt atacaaatat gggtagatat aggaagtaat gcttggaata tgaaaataga
     6301 aaaagatata agtaatttaa ttgtacttag aaaatctttt gaagaaaaaa agatttatac
     6361 aaatgaaatg gaagaatatt ataaaaaata ttatctatac aatacagtat ataatttgtt
     6421 attaaatgca aagaaaatct atgcatttaa aagtgaatat gcgattaatg gttatatcca
     6481 agagagtata ttatatagta tacttttaaa aatctcacat aaaggagata aaaattatga
     6541 ttaatataaa ttatagagaa gaccagatac caataataaa ttacgataat ggtactatgg
     6601 cagtaccagc agtaccaggt gctggaaaaa catttataat aacaaattta gtagctaaac
     6661 tattacttga acagaaacat aagagtggta aaatattaat tcttacatat atgaatagtg
     6721 ctgtaaataa ttttaaaggt agaataagaa agattcttga agagaataaa atagatgata
     6781 ctaattcgta tgaagtaatg actatacaca gtttggctgt aaaaataata aaggaaaagc
     6841 cagaagttgt aatgttaagt gaggatttta acatagctga cgatttgcaa aaaagtatta
     6901 tattaaatga atgtataagt agatttagat ttgaaggtgg agaaagagct tttagatggt
     6961 ttttaaagga gcaaaaagat gaacgctgga aagaaataac tcttgaagcc tgggaaagag
     7021 gtttttttga atttgcagga aatgcaataa gtgaacttaa gtataaggga atttctccag
     7081 ataaacttga agaaatattg tcaagaggac acaaaggaat gcttaaaatt attttaccaa
     7141 tatataaatt gtatgataga aaattaaagc aaaatggatt gctagattat gatgatatat
     7201 taattttagc agaaaaaact ttaagcttag acgaagggct tagaaagaag tttcaaacta
     7261 gatacaaata tatatttgaa gatgagtgtc aggactcaaa tgaaattcaa ggtaatataa
     7321 taaaaattat atctagcgaa aataaaaacc tagttagagt tggagatata aatcagagta
     7381 taacaggaac attttcatca tcagacccta aattttttaa agaatttata aatagtgcag
     7441 atttctgtta taggatggat atgtcaaatc gaagttcaaa agatatactt gatttagcaa
     7501 acactcttgt caagtatgtt acaaaggaat ttagacaaga agaatgtaga catgctcttg
     7561 aagatatgca aataaaaact gtgccaaatg gaatgggata taaagaaaac cctaatccag
     7621 aacattataa tataaatgta aagtggtatg agagttggaa aattgaaata gagcaaactg
     7681 ctagatacgt taagggtata aaaaataaat atccagataa aagtataggt atattggttc
     7741 catttaatga acaagtgaca caagttgcta aagaacttag agagaataat ctaacctttg
     7801 aagaattagg accaaattct ttgaataaaa ggaaggttat aaacaacata gcatatatta
     7861 ttgattttat gttacattgt gatgatgtag aaaagttaat agttgtactt aataaagttt
     7921 ttatagatac tgataatgaa gaagaaataa agtatttttt agaagaatta aaaaactatt
     7981 ctaccgaaga aattatttat aattttgaat ttagtaagtt agataatgat ttaatagaat
     8041 ttaaagaaga aagtgatata tacttaagtt ttaaaagagg aatagatatt ataaaacaga
     8101 tattagaata tccaataata aggttagacc tcttagtttt gtttattggg aaaaaactta
     8161 gtttagaaaa agaagataaa gctgtactgg attatatatc tttttatata aagtatctag
     8221 tatcagaaaa tataaatatg gatttagaag atgtatataa tttattgttt gatgtaaaga
     8281 acagggtgtt taatcatata atagatgtgg tatatgagat aaatggatat gaaccagagc
     8341 ctggtagtat aacactatgt aactatcata aatcaaaggg gatggaatgg gattgtgtgt
     8401 ttttactagg tcttgttgaa tataatttcc ctgacaatat taatcaaaag tttcagagtg
     8461 ataaatggta tctaaaagaa aaatataaaa accctatggc aattattaaa tcagaagttg
     8521 aagctatatt aaaaggaagt atttcaacag attatgctca taaaacgaag attgattcta
     8581 taaatgaaaa gattagactt ttatatgtag ggattactag agcaaaggaa atgttagtac
     8641 tttcaggaag tgcttataga gatgaaagtg atatagggaa taagagaaaa gagcaaaaac
     8701 catgtatata tttaagcaga cttaatcaac atattattga aaaaaggtca aattaatata
     8761 taataagtta ttaaaagatt gtttgaaata tatctaaaag aggataaaac aatatgtaca
     8821 gtaagtaact actaaaaaat aaagttaaga gagaagtgat atgagtgaac gataaattaa
     8881 aatatttttc ttatagtcaa aattcaatta acacatataa atcatgtcca ttgaaattta
     8941 aatataaata tattgataga ataaattgga aaaatgatga tgttggtagt agagagtatt
     9001 atgaaacttt aaaaacagga agagattttc atttgatttg tgaaagatat tttagcaata
     9061 tacctcttgg aatctatttt aatgaggatg ataaaaatag taaaaagttt ttaaagtgga
     9121 tggaaaatat aaaaaaagtt gttcctattg aaaaaggaaa aacgtatttg ccagaatatg
     9181 aagtaaggat gacattaaat ggggatataa tacaggcaaa gtatgattta gttgtaattg
     9241 gaaataacaa tatagaaata tgggattgga aaaccgagtc taaaaagatt gactataaac
     9301 acgtagaaaa tagaatacaa actattgttt atatgttttt agcaaaagaa gtaataccta
     9361 aagttttaaa aatggacata aatgttaaag atataaatat gagatattat caacctgaat
     9421 ttgatgactt accaattacc attttatatg atgaagaaaa acatgaagca aatagaaata
     9481 aaattcaaaa ttatataagt atgataaaaa acactaatta tgaagaacat acatacgaag
     9541 acaatctata caatgatata gaaaaggtat ataataaaga agaacgtatg tgctatagaa
     9601 ataaaaagca ttgcaagtat tgtgaattta ataagttatg taatggcaaa gaaatagatt
     9661 atagtatttt ggaggcggaa atttatggga cttaggtttg tattaggtag aagtggaagt
     9721 ggaaaatcca cctatatact agatgagatt aaaaaagaag ctcaaaaaaa tgaaacgaca
     9781 tcaattatac ttttagttcc agaacagtac acatttgagg ctgaaaatag agtaagtaaa
     9841 ctatttttag gaaaagaaaa agataagtat ctaagagtta gggtacttag ctttaagaca
     9901 ctaagtaata tagttttttc acaagtagga ggattgacag atgtaaatat aaattctagt
     9961 ggaaaagcaa tgatggtata tagagctatt gaagatgtaa gtgaagaatt aaatgtattt
    10021 tcaaaatcaa aatcgcagtc tggttttgta agctcaatta cagatatgat atcagaaatg
    10081 aaacaataca atataagccc tgaaatgtta gaaaatatat ctggagaatt ggataatgaa
    10141 actttaagtt taaaattaaa agatataagt aaaatatata attcttttga agggaaattg
    10201 catgaaaatt atgtagatgc tcaagatatg ttaacttcat tggcaagtaa aattgaatta
    10261 agcagttatt tagatggagc ttgtgtttat atagatgaat ttacaggttt tacaccaaat
    10321 cagtacaatg tcataaagag tatattaaat aagtctaaaa gtgttaatat ctctttgact
    10381 gtagatgata tcaactatat tgggtacagt aaatctgata tgttttccag aacgaaattt
    10441 acttattcaa aattgactca attgtgcaac gaagaaggca taaaaatatt gccacaagta
    10501 aatttaaaca caggtgttat aaaaagattt gaaaaagtta aggaattaca gcacttagaa
    10561 agattttata atgcttatcc atataaaata tattcaaatc aaactgaaaa tataaagata
    10621 aaagagttta acaatttata ttcagaggtg gaagagatag ccagagaaat tgtacattta
    10681 gttagagata agaatgtcag atatagagat ataaccattg ctacaagaga tttaaataga
    10741 tatgattttt tagtacactc tatatttaat gaatataata taccaaactt tatagataag
    10801 aagagagagg caaaaagtaa tccaatagtt attttaatta tttcagcttt agaaatgaag
    10861 aatagacgat atgggtatga aactatgttt aggtatttaa aaagtggact tataggaatt
    10921 gataatgatg atataaattt actagaaaat tatgtgttgg ctaatgggat taaaggtaaa
    10981 aaatggtttg atgaaaaatg ggattataga ataacccaaa gtctttcagg tcaagaaagt
    11041 gaatttgaac tggaattaaa agagaaaata aatgaaataa aaaatagagt attagagcct
    11101 atagtaattt tacaagaaaa attaagagga aaaaatagag taaaagaaat ctgtagatat
    11161 atatatgaat ttttattaga tataaatatg ccagaaacta tagaaagttt aataataaat
    11221 tttaaggata aaggggaact tgatgttgca aatcaatact cacaagtttg ggacatagta
    11281 gtggacatat tagaccaaat ggtggaactt atgggtgatg aaataatttc tttagaaaaa
    11341 tttataaaat taataacctt aggttttgat gaatatgaat taggactggt tcctcctagt
    11401 atagaccagg tacttgtaag tagtgtggat agaatgaaaa atccagacac gaaatattta
    11461 tacctagttg gaactactga tggtgtgttt ccgctgatta caaaggatag tggaatattg
    11521 agtgataatg atagggaaag tcttggaaac aaaggaatcg aagttgatat tgatagtaag
    11581 actaggagtt ttgaagaaca gtttcttgta tataaagctc ttacttcgac atctaagaat
    11641 ttaaccataa catatccaat atcagaccat gaaggtaaaa cgcttagacc atcaataatt
    11701 atatcaagac ttaagaagat atttccaaat atagaaaata aaagttattt agtggaagaa
    11761 aataaaagca ctgataaaga tatacttaaa aaaataacgg ttaaatcccc tacatttaat
    11821 gaacttataa acgtaataaa aaattatgac agtgatggtt acaatacaga agagattaat
    11881 agtatttggt tagatatata taggtactat ttaaaagatg aaatctacag ttctataaca
    11941 aaaaaagtta taaaaggtct tagttatact aatcaagttc ataagattga agagaaaaaa
    12001 ataaggtcat tgtataaaag taattctctt agcatatcta ggcttgaaaa atatgctgaa
    12061 tgtccatttg cttattttat acaatatggt ctaaaagcaa aaaagcgtaa agagtattca
    12121 tttacaccac cagatttagg aacttttatt cataatattt tagataggtt ttcaaaagaa
    12181 cttttacaag ataatttaac ttggagagat attgatgaaa aatatataga attaaagata
    12241 ggtataatag ttgatgaaat aattttaaag ataccaggat atatattaaa tagttctgag
    12301 aggtataaat accttgcata tagattaaag aatatgttga caactgctat aactataatt
    12361 agtcagcaaa taaaacaagg ttcatttgaa ccaatagatt atgaagttaa atttggcaac
    12421 aatggaaaat atccacctat aaaaatggtg ttagaaaatg gacaagaagt aagtttaatt
    12481 ggacaaatag atagagttga tgaatttgaa gaaggagaaa ataaatatat aagaataata
    12541 gattataagt caggcaataa gtccataagt ctaacagaaa tatattatgg tctacaatta
    12601 caattacttg tgtatttaga tgcaatcctt gaaagtgcaa aggatgaaga tataaatata
    12661 aatccagctg caatactata ttgtagaata aataatccaa tagcaaaatt taatgaagat
    12721 aaagatgatg aagaaataca agaagcaata ttaaaagaac ttagaatgaa ggggcttgta
    12781 gtaaaagact ctcacatagt taaagagatg gataaatcat taatagatgg agaaaggaaa
    12841 aactctttag taataccagt aggtcttact aaggatggaa atgtaggtaa aagtacttca
    12901 gcaataagct atgaggattt caaactgctt agaaaatatg taaggcatgc tataaaggat
    12961 ttatgtgaag aaatgttaag tggagaaata cgtatagctc catataaaca taaagatgga
    13021 acatcatgtg acttttgtga ttactctgct atatgtcagt ttgattcaac tataaaagat
    13081 aacaagtata aaaatttaaa taataagagt aatgaagaga taataaaaat gatgaaagga
    13141 gatgttaatt agtgagttct cctaaatgga caaaagagca attagaagtt atagaaagta
    13201 gagaatgcaa tttattggtt gctgctgctg ctggttcagg aaaaacagcc gttctagttg
    13261 agcgtataat tcaaatgata acaagtagag aaaatccaat tgatatagat aagttattag
    13321 ttgttacatt tactaatgct gctgcgtcag aaatgagaga gaggataggg gatgctatag
    13381 gaaaagcctt agatgaaaac ccagaaaata aacatttaca aaaccaactt gttttattaa
    13441 ataaatctag tattacaaca atacactcat tctgtttgga tgttataaaa tcaaactttc
    13501 atagaataaa cttagaccct aatttcagaa taggagacca aactgaatgt gctattttga
    13561 aacaagaagc aatagaggaa gtatttgaag atttatatga agaaagagat gaggggtttt
    13621 taaatttagt tgaaagttat gcagaaagag gaggagataa agaagtacaa gatattatct
    13681 tgggaatata ttcatttgca atggcttctc ctgaacctaa aaaatggttg attgactcag
    13741 cagaacgatt taatattgat gaaaattttg atttttcaca gtctatttgg gctagagcta
    13801 tattagatac tgtaaaaata gagattaatg gtctttgtct aaatatggaa agggcattaa
    13861 aagaagtgga atcaatagaa gaattagaaa cttttgcaga aaagttatcc gttgaatata
    13921 aaaaaattgc tgacatttca caagcatgta ataaatcttg ggatgaggca tataaaaaga
    13981 tggcaagtat gtcctttgaa aattatgtaa aaggagtaaa aagaatttca aaagatgctc
    14041 cttcgtatat aaaagaatca aaagaaaagg caaaaactat aagagataag actaagaagt
    14101 ctttagaaag tatagtaagt gctacattca ataaagataa tgattctatt agagaagaaa
    14161 taaaatatct ttataacata gttaaaccaa tatctagtgt tgtactgaga tttgaagagg
    14221 aatatagcaa taagaaaaga gaaaaaggaa taatagattt taacgatata gagcattttg
    14281 cattaaatat acttacagat gtagatgaaa aaggtaatat tgtaccttct gatatagcag
    14341 ttggatatag aaacaagttt tatgaaatat ttatagatga atatcaggat agtaatctag
    14401 tgcaggaagt actgcttaaa gctgttgcaa atacagaaac tccaaataga tttatggttg
    14461 gtgatgtaaa acagagtata tatagattta gacaagcaaa gccagaatta tttttgcaaa
    14521 aatataataa ctataatgat aaaaagggaa gctcccatag aaaaataatg ctttacaaaa
    14581 actttagaag tagagaagaa gttgtagatg ctgtaaatta tatatttgaa aatataatga
    14641 atgagaatat aggagaaatt gaatatacag aaaaagaaag actaaattta ggtgcaaatt
    14701 ttaatgtgga tacagacgag aaatctatta ttggtggagc aacggagata cacctaatac
    14761 aaaaagataa taaacttgat gatgatatta taaatgataa agacgataga attaataaca
    14821 aagaaaatga aatagaggaa gaagaaaaat tagataatat acaacttgag gctagaatgg
    14881 ttggaaacat tatcaaagat ttaatgaaag tcaatgaaga tggaaaaatt caaaaggtct
    14941 atgataaggg aatagatgga tatagacctg tggaatttag agatatagtt attcttttaa
    15001 gagctacatc tgcatgggct ccagtgtttg cagatgaatt gatgaatatg gatataccaa
    15061 cgtatgctga tgttggagtg gggtattttg atacgattga aataaaaaca atattgtcat
    15121 tacttcaaat aatagataat ccaatgcaag atatacctct tatatctgta ttaaaatcac
    15181 ctatatttgg atttacacca gaggatttga ttgatattag agttcagagt aaagataaaa
    15241 ttttttatga ggttttaaaa agtacagcag agtatgatgg atttacagat tctcaaaatg
    15301 aaaatgagtc tgagtttata ccaagtgaag aatgcataaa caaaagtaaa gattttttaa
    15361 ttaagctaaa ggaatttaaa gaaaagtcaa tgtacatgag tacagatgaa tttatatggt
    15421 acttgtacac aaggactgga tactatgcct atgttggagc attgccaggt ggaagtcaaa
    15481 gacaagctaa tttaaaagta ttgtttgaga gagcaaaaca atttgaagaa accagtctta
    15541 aaggaatatt caactttgta aattttatag agaaattaaa aaaatctagt tctgatatgg
    15601 gaagtgcaaa aacacttggt gagaatgcaa atgttgttag aataatgagt attcataaaa
    15661 gtaaaggtct agaatttcca gtagtcatat gctctgcaat gggtaaaaat ttcaatactc
    15721 aagattttaa aaagagtatt ttatatcatc ataatttagg gtatggacct caatttgtag
    15781 actatgagag aaggatttct tttccaagta tagccaaaga agcattaaaa agcaaaataa
    15841 atatagaaaa tttatcagaa gagatgagag ttttatatgt tgcatttaca agagctaaag
    15901 aaaaattgat tataactggt tctactagaa atatacaaga tagtataaag agatggtcaa
    15961 atggaattga aagtttagat acaatatcac aatacgagat attaaaaggt aaaaattttt
    16021 tagattggat aatgccttgt gtattaagac atagggattt atctaatcta ctagaagaag
    16081 taggattaga cgcagttttt aatgttgaac ataattcaaa atggtatgga aagttgtgga
    16141 ataagagtga tattttagtg gaaaagaaga gtgatgaaga aaaagaaagt attgaagaaa
    16201 ttcttgaaaa aatagatgta gataatcctg atagtgatta ttacagtgaa atagaagaaa
    16261 aattaaacta tatatatcca tatgaattta gtacaagaaa acctgctact atatctgtta
    16321 cagaaataaa aaagatacaa aataattatg aagaagagtt aataaatact atatttgaac
    16381 aaaaagtaat attaaaaaag cctctattta ttcaaaatga agaagaaaga gaaaaaataa
    16441 gtggaacaga aagaggtaca atagtccatt tagttatgga ggttttagac ttaaaaaatg
    16501 taagtagtgt aaatgatata aaatcacaaa taagaggttt tgtttcaaaa ggtataataa
    16561 cagaaaaaca agctagtata gtaaatccat ataaaatata taaatttttt gcctctaaca
    16621 taggaaaaag aatgttgaat gctgaaatta taaatagaga aaaatcaatt tatgcacaag
    16681 taaatatgaa agacatatat atttatgaaa agcttataaa caatgacgat aaaaaacttt
    16741 atgataatga aagtgtcatg ttaagaggta tagttgatgc ttattttgaa gaagacaatc
    16801 aaatagtttt ggtggattat aaaacagatt ttgtaaatga ggaaaatata aatcaaataa
    16861 tagagaaata taaaaaacag ttggatttat atgcagatat tattgaaact ttaactggta
    16921 agtcagtaaa agagaaatgt atatatttat ttggagttga tgaagctgtt tgttattagt
    16981 ttttatatat aaattaaacc taaaagatag aggaagggga aaaaatgaga tttattcata
    17041 catcagattg gcatctaggg aagtccctag aaggccattc tagaattgag gaacaagcaa
    17101 agttttgtga ggaatttata aaaatagtag aatcaaatga aatagatatg gttataattg
    17161 ctggagatgt atatgatact tctaatccac cagcacaagc agaaaaacta ttttatcaga
    17221 ctgtatcaag acttgccaat aatggtcaga ggtgtgtatt aataatttca ggaaatcatg
    17281 ataatccaga gagactctct gcaataacac ctctagcaca tgaacagggg atacttatat
    17341 atggttatcc attaagtgct acgatagaag ctaagtataa agggtttgaa ataacatatg
    17401 caactcaggg atgtacaaaa ctgaatataa atggagaaaa tattgtggtt gctacattac
    17461 cttatccaag tgaaaaaaga ttaaatgaag ttttttcaag tgaagatgag tttgagaaac
    17521 aaaaaaatta ttctgaaaag gtaggagata tatttagaag tttagaagaa aattttagaa
    17581 gtgatactat aaatatagcc gtatcacata tatttgtaat aggtggagag agtactgaat
    17641 cagaaagacc tatacaatta ggtggaagtt ttttagttga aagaaaagat ttgccagaaa
    17701 aggctcaata tactgcacta ggtcatttac ataaacaaca aaaagcttcc gaacgtttaa
    17761 atgcatacta ttctggttct ccacttcaat atagcaagga tgaaagagcg tataccaaag
    17821 gggcatacat agtggatata aaagctggtg aaaaaccaat aattgaagat gtatatttta
    17881 ataattacaa acctatagaa gtatttaaat gtaatggaat agaagaagca ctagatatat
    17941 gtgagcaaaa tcaagataga gagatttggt catattttga aataaatact gatgagataa
    18001 tatcacagaa tgaaattaaa aaaatgaagg aactattaaa agacataata gaaataaaac
    18061 caataataac atcttgttat gaacaagaaa gtgtagatat aaaagaaaag tctatggcag
    18121 agttgtttag agagttctat tcttttagta aaggtgtaga gccaaaagga gaacttatgg
    18181 atttatttct agatataata agtgaagaag gtgaatcagc agatgagacc aattagatta
    18241 gaacttacag ggcttaatag ctatattgat aaacaagtta tagactttga aaaattaatt
    18301 gaaagaggtt tgtttgggat atttggaact actggaagtg gaaaatctac aatcttagat
    18361 gcaataacta ttgctatgta tggaaatata tccagaaata caaaagaata tataaacagt
    18421 gtgtgcgata aggcaattat atcatacgag tttgagatag gaagcaaaaa cactagaaga
    18481 agatacattg tagatagaac cattgctaga agcaaaacag gaacaaagac ctctaatgct
    18541 agattaatag aagtattgaa tgacaatacc caaaatgttt tagctgataa agttgtggaa
    18601 gtaaatgaaa aagtagcgca ggtcgtagga ttaacggcaa atgattttac taggtcagta
    18661 gttttaccac aagacaaatt taatgagttt ctaagattat cacgtgcaga tagaagagac
    18721 atgttagaga gaatttttaa tcttgagaaa tatggaagaa gtcttggcga aaaagtgaaa
    18781 aagagaaaaa atatacagtt acaaaattta aaagatttga aatcaaaatt aagtcaatat
    18841 gatggaatta cagaggaagt atataacaat ataaaccaag aattaataga gttaaaaaat
    18901 ttggaaaaag ataagaataa tgctttagac ctagctcaaa aatcttatga agaaagtaaa
    18961 acagtatatg aagaacaatt aaagttagaa aaaaatgaac ttagaaaaaa tgagcttgat
    19021 ttaaaaaata gtgagataaa agaaaagatt gctcttgtag aaaatgatag taacgctaaa
    19081 aaggtagacc catacataag ttcagttcaa aatctagaaa aaaagataga tgaagatagc
    19141 ttcgcagtaa gtaatttaga aaagaaatta gcaatattaa atcaggagct tgaagttaca
    19201 aaaaatagat atgaaaaaat aaacaaaatt aagaatgaag aaatccctaa attgagtgaa
    19261 gaaaaaataa gattgcaaca agcaataaaa ttagaagaag agttagtttt attagataga
    19321 gagttaaaag agctaaaaga aagtggaatt aatttaaata agactaaagt tgaattagaa
    19381 aaagtaaagc aggtgtcaga gtccagaaaa gatgcagtta ctaaatctat aaaagaagtt
    19441 gaaggaaaga ttgataaagt aaatataagt gcagaattaa aacaaaaaat atttctagca
    19501 tatgaatacg agaaagatta caacaacgta ttggaagaaa aaaatcaaaa gctaaataaa
    19561 ctagaagaaa tactcaaaga gacagaaaat ataaatttaa aagttagata tattgataaa
    19621 gataagaatg atgtaaatcg taatttagaa aatttatcac ttcacttgga tgttcttctt
    19681 aaaaaatgtc ctggtaagag tgctgattta cttttaaaat ctgaatatgt tactgaatta
    19741 agaaataaag caaataatac taaagaaaat gaaattaaga aaagcagtat acaagatgaa
    19801 ttaaagataa tacttgaaag taagtttaat actgaaaggg aagtaaattt actaaatgaa
    19861 aaacttgaaa ataatagaaa gaacagagat gacttagaaa aagaattaga agaactaaag
    19921 tatttaaatt tagcctctga acttagacgt gaattaaaag aaaatatgcc ttgtccagtt
    19981 tgtgggtcaa aacatcatga aaatcatgat ataactaagt atgatgaaaa tatatctttt
    20041 gtcaaagaaa aactggagaa attagaaaaa gaaaaaataa gtataagaaa caatatagag
    20101 gaattaaatg ctaaagtaag tgggtattta tctatagaaa aaatgaagac taaagaactt
    20161 gaagatgtaa aaggaaaact aggggggatt ccatctagtc aattattaaa aaaacttgat
    20221 gaagaacaaa gaaaacttgc attgttaaaa tctaatattc aggagtggga gaaagaaaaa
    20281 gagagtactg aaaataaagt aacattagct aaagaagaaa aaaataaaat tgaaaaagaa
    20341 gaattaaaaa taagagagag tctgaataat tataagaaat taacaagaga tttaaacata
    20401 gaaatagaag gtttagaaaa taaatgtaaa aaacttaaac aggaatacct aggattaaag
    20461 acgattacta aagtctctaa tctattgtca aaagtaaaag aaataaggga gaatgaaaaa
    20521 gaattagaat tactaagtac tgggcattca aatttattaa aaaatagaga ctcactagat
    20581 attaaaataa gagaaaatga gagtaaacta catgaaatag agatagaatt aataaaggct
    20641 agagaattat atgtagaaaa gaaattgagt agagataata agtataaaga agttattagc
    20701 ataacgaaag gtgatttagc taaaaatctt ttacataatg ttgaagaaaa tatatgtaag
    20761 atattagaac aagaagaatc aagtaagaaa aaattggaag agcaaagatt agaatatgaa
    20821 aaaaacattg ctgaaaagca caatatagat ggtagactta aaactgctaa ggaacaatat
    20881 aaagaccaaa aatcaacact aaataaacta ttggcagaaa ataaatttga aagtatatat
    20941 gcagtaaaaa gagctttatt agatactgat gcagtaaaaa aattgattga agaaatacta
    21001 gagcatgaag aagaacaaaa gttattatca tttaaaataa aatcatcaaa agaaaaatta
    21061 aatggaagaa gcataaagaa ggagtacttt gaacagttaa aagatgaaat ttataatttg
    21121 aaagtagaaa taggaaatat atccaaagat attggagcta atcaaaatca gttaataaca
    21181 ttaaaagact ctttagataa gataaatgat tttaataaac aacttaaggt agttgaacac
    21241 aatgtggact tattggaaga attagataaa tgtgtacaag gaaataaatt tgtagaatat
    21301 gtttctacaa accaattaaa atatatagcg ttggaagcat caaaacgatt ggatggaata
    21361 acaaaaggaa gatatgcact tgaaattgac tcaacactaa actttgtaat gagagataac
    21421 tttaatggag gagaaagaag aagcattgat actctatctg gtggagagac attcttgaca
    21481 tctctatcat tagcacttgc tctttcatct caaatacaat taaagggaag tgcaccatta
    21541 gaatttttct ttttagatga aggttttggt tcattagata gtgaactttt agaaattgtt
    21601 atagaatcct tggaaagatt gcatagtaat aatctaagtg tgggtataat aagtcatgtc
    21661 gaggaactta aaaatagagt tcctgttaag ctattagttt catctagtga ggctggaata
    21721 ggttcaaaag taaaaattga atatagttag aaaatatatg ttaattgaat atattatcta
    21781 atatttccaa ataatatgta attgtgatat aataaaaact aatattaaag ttatttataa
    21841 ttaatattaa tataaaaaca tgtaagtagg agtgtggtaa gtgttggaat cgcccaataa
    21901 tttgattcag attatttttt taatagtatt acttataggg tctgcatttt tctcagcatc
    21961 tgaaacggcc ttaatgtcgt tgagtaaaat cagaatcaga tatatgcaag atgaaggagt
    22021 taaaggagct aagttagtaa gttcattaat agaaaatcca aataagcttt taagttctat
    22081 attggttgga aataacgtcg taaatatagc agcaacatcc atatcaacat ctttgtttat
    22141 aggattaatg ggagaaaaag gagtggcact tgctacagct gttatgacag tattagtatt
    22201 aatatttggt gagattactc caaaaacaat agcagcaaat aattcagaaa aggtttctct
    22261 attagtatct aaacctataa aagcaattat atttatatta agaccaatag tatggatatt
    22321 taatataatt acaaatatta tatttaaatt atttggtata acaaataaag gagctaaatc
    22381 attcataact gaagaagagc taaaaactat ggtaaatgta agtcatgaag aaggcgtcct
    22441 tgaaatggaa gagagagaaa tcataaataa tgtttttgaa tttggagata tgcaagctaa
    22501 aaatgcaatg gtacaaagaa tagatatggt agctatagat atggaagata gttatgatga
    22561 gataatacag gtatttaaaa ctgaaaaatt aagtagaatg cctgtgtatg aagaaactat
    22621 agatgatatt gtaggaatac ttaatataaa agatattata tttttatctg atgaagaaat
    22681 agagtcattt gatattaaaa attatatgag agaacctttt ttcacgtatg agttcaaaaa
    22741 gataacacaa cttcttgaag agatgaaact tgaaaaaagt cagatggcta tagttgtaga
    22801 tgagtatggt ggaacatctg gacttttaac tatagaagat ttagtagaag tcattgttgg
    22861 agatatagag gatgaatatg atgaagaaga ggatgaaata caagttataa aagaagatga
    22921 gtatattgta gatggaagta ctaaaattgg tgatgttaat gaacttattg gtgtaaactt
    22981 ggaatctgag gagtttgatt ccataggagg ttttataatt ggacacctaa gcagattgcc
    23041 agaagaaaat gaagtaattg aagttgataa tataagattt tgtatagaaa gtatagaaaa
    23101 aaatagaatt aaaaaaataa gaatatatac ataatgtaat aatgttctta aatttattag
    23161 aagtgtataa atataaaaga tatcttttat atttatacac ttctttttat tttgtattca
    23221 aataaaacta gtatgaaatg gacaaaataa aaatataaat tagaagaaag gagattttta
    23281 agatgagcat actagaaaat aattttttat acaaattaaa aaacaacact atcttttttt
    23341 gtatatatac attgttattt atttttattt acaaagcgtt tccatacata gctccatttt
    23401 ttctaggtgc tataatagca cttatgatta atcctatatc acaaaagtta gaaaataagt
    23461 ttcatataaa taaaggtatc tctactcttg ttctaagttt tcttgcagtg gctatagtca
    23521 gtacagttac tacaataata gtaataaatt caatgaaaga attaatggga tttttaaata
    23581 atatatctgc taatcctgag gatataagta atactattat gtatttttta aataaaataa
    23641 atgactttat gaaatcattt caagaaatag ccaattttga tgtagaacaa ttagtaaaca
    23701 aattcagtgg tgaagtaatg caaattacaa aaaatcttct tactagtata cttggattgg
    23761 ctacttctat tccatatatt ataatattta taattacctt gtttattgct acgtatttta
    23821 tagcaaaaga ccttgataag attgaaaata gtttttataa tatgtttact gttgatgtta
    23881 gaaagaaggt aaaaaacgta aagaaagagg cagggctttc acttgttgga tatataaggg
    23941 cctatacaat acttatggct ataacatttt ttgcaatctg gggaagtttt gctttatttg
    24001 gattgaaata cggtctaata gtagggtttg taggagcttt aatggattta attccattcc
    24061 taggtataat aacaatatat cttccagtaa ttgtttatta ttttcttata aaaaattatt
    24121 ttatagctat aagtatgact gtgatatttt ttgttttgtc tttgattaga gaaatacttg
    24181 aacctaaact agtatcagta aatgttggac taaatccatt agctacccta gcagcgattt
    24241 ttattggaat ccaagtaaaa ggaataattg gagtaatttt ttgtttgggt ttggtatgta
    24301 tgcatgatat acttaagaag gtggatattt tttaattata ataattgata gattttttaa
    24361 aaataaaatt ttattcacag gtaaataaaa aaatattaat cttattaaga aagagggtat
    24421 atacgtgaaa caaaaagtag tggaaagatt tttaaaatat gtatcatttg atacaacatc
    24481 taattctcag tgtgagaact gtccatcttc agaaggtcaa agagttttag ctaagtatat
    24541 agttgaagag ctaaaaacaa tgggtgttga tgatgtgagt ttagatgaaa atagttatat
    24601 aatggctact ttaaaaggaa atactgatgg tgtggatact ataggattca tatcacattt
    24661 agatacaatt gaagatgtta gtggaaaaga tattaaacca aaaataatag aaaattatga
    24721 cggaaaagac atagttttaa atgaagcgtt aaatgttatt acgtatgtaa aggattctcc
    24781 agagttggaa gaatttaaag gtgacgattt aatagtcact gatggaacaa ctctacttgg
    24841 gtctgatgat aaagctggta tagctgagat agtgactgca atagagtatc taataaatca
    24901 tccagaaatt aaacatggag atataaaaat aggatttaca ccagatgaag aaattggtag
    24961 aggtgctgat ttatttgatg ttgaaaaatt tggagcaaaa tatgcatata ctttagatgg
    25021 aggaatagtt ggagaacttg aatgtgaaaa ttttaatgct gcaaatgcaa ctattactat
    25081 acatggtaga aatgttcatc caggttcagc taaaaataaa atggtaaatg ctatacatat
    25141 agctgctgaa atatcagaaa tgttcccagc agatgaaaga ccagaaacaa cagaaggata
    25201 tgaaggattt tggcatttaa attctatagg tggaaatgtc gagaatgtaa gtatggctta
    25261 tattattaga gaccattgta aagaaaaatt tgaaaataga aaatctatta tgatagagaa
    25321 tatagaaaaa ataaataaga aatacgataa tagagtggaa ttggatttaa aagactctta
    25381 ttacaatatg aaagaaaaga ttgagccagt tatgtttata gttgatatag ctaaagaagc
    25441 tatggaggaa ttaggtataa aaccacgttt agttcctgta agaggtggta ctgatggagc
    25501 aagattatct tttaatggat taccttgtcc aaatatattt actggtggat taaacttcca
    25561 tgggaaaaat gagtgtatac cagttagttc tatggagaaa gctactaaac taattgtaag
    25621 aatagctgaa aaatatgctg aaagagttta gttaaaaagt atttaaaaat taagcattta
    25681 aagttataat agctatatca ttctttataa gtcaagctga ttataagctt gtgttgtact
    25741 taaaatggta tagctatttt tgtaattatt tttataaaag ataattattt ttagtttagg
    25801 gtatcaataa aaagtatgaa tatcaatagg tattaacatt taaaaggggg aattgtattg
    25861 aaaattaaat ctaaaaaaat aatagcattt gcactttcac ttgggattat gttgccaagt
    25921 tgtgtatatg cagacaatat tagtaaaaat caaaatgcac aggtagatat gaacaattat
    25981 cttcaaggta gaaaatctcc aaaggaggaa aaacttattg gtgaagatag atttgataca
    26041 gcgataaaaa tatctcaaag tggttggaat aatggttcag aaagggtgtt tctagttaac
    26101 tcaaattcat tgccagatgc attggcttca actcctttgg caagtaaatt ggatgcacca
    26161 atactgctta caaataaaaa tagtatacca tataatgtaa cagaggaaat aaaaaggctt
    26221 aatccagcag aagttatttt aattggaagt gaaggagcta tttcatcaag tgtaaaagaa
    26281 tctcttgaag atatggaagt attagtcagt agaatcggtg gagcagatag agaagaaaca
    26341 tctttacttc ttactagaca gctagatgat actggtgatt taggtgtctc aaaagtggca
    26401 gtggttaatg ggtataatgg actggcagat gcaacttcaa tatcaagccc agctgcaagt
    26461 gataatacag ctataatata tacaggtaag gatagcataa gaagtgaggc aaagaatttt
    26521 attacacaaa actcaactaa gacatacata gtaggtgggg agtacagtat aagtaaaaaa
    26581 cttgaaggac agttagtaaa ctcagaaaga ctagcaggga cagatagaaa agatactaat
    26641 gcgaaagttt tagaaaagtt ttatggaaaa tcaagtaagg ttaataatat gtattttgca
    26701 aaagatggaa gtggtagaga agctgattta gttgatgggc ttgctgctgg agtattagca
    26761 tcaaaaacaa agtcacctgt agtacttgca agtggaagtt taagtagtgc acagaaaagt
    26821 tttataaaaa aagtaaaggc tgataaattt gtacaagttg gtggaggtaa aaatagtaaa
    26881 ccatatgcag aagcattagc cttacagtaa tattgtgtaa atcaaaatta atatataata
    26941 atagcaagta aaccaagttg cactttaata tatataataa aataagtaaa
//
LOCUS       DACULQ010000030        26927 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.030, whole genome shotgun sequence.
ACCESSION   DACULQ010000030 DACULQ010000000
VERSION     DACULQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.030"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(46..1650)
                     /locus_tag="JA154_05475"
     CDS             complement(46..1650)
                     /locus_tag="JA154_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7995309.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2036..2962
                     /locus_tag="JA154_05480"
     CDS             2036..2962
                     /locus_tag="JA154_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase"
                     /protein_id="HAT7995310.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTSDSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNTYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3082..4080)
                     /locus_tag="JA154_05485"
     CDS             complement(3082..4080)
                     /locus_tag="JA154_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT7995311.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4184..4621
                     /locus_tag="JA154_05490"
     CDS             4184..4621
                     /locus_tag="JA154_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7995312.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            4707..5294
                     /locus_tag="JA154_05495"
     CDS             4707..5294
                     /locus_tag="JA154_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HAT7995313.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(5594..6214)
                     /locus_tag="JA154_05500"
     CDS             complement(5594..6214)
                     /locus_tag="JA154_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 domain-containing protein"
                     /protein_id="HAT7995314.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(6222..6989)
                     /locus_tag="JA154_05505"
     CDS             complement(6222..6989)
                     /locus_tag="JA154_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HAT7995315.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7030..7851)
                     /locus_tag="JA154_05510"
     CDS             complement(7030..7851)
                     /locus_tag="JA154_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995316.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(8210..10120)
                     /locus_tag="JA154_05515"
     CDS             complement(8210..10120)
                     /locus_tag="JA154_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate
                     aminotransferase"
                     /protein_id="HAT7995317.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10399..11184)
                     /locus_tag="JA154_05520"
     CDS             complement(10399..11184)
                     /locus_tag="JA154_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAT7995318.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPISIDAMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKAYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11401..11631)
                     /locus_tag="JA154_05525"
     CDS             complement(11401..11631)
                     /locus_tag="JA154_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HAT7995319.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(11650..12798)
                     /locus_tag="JA154_05530"
     CDS             complement(11650..12798)
                     /locus_tag="JA154_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAT7995320.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(12798..14312)
                     /locus_tag="JA154_05535"
     CDS             complement(12798..14312)
                     /locus_tag="JA154_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HAT7995321.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14433..15623)
                     /locus_tag="JA154_05540"
     CDS             complement(14433..15623)
                     /locus_tag="JA154_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAT7995322.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(15911..16930)
                     /locus_tag="JA154_05545"
     CDS             complement(15911..16930)
                     /locus_tag="JA154_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT7995323.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(17106..17645)
                     /locus_tag="JA154_05550"
     CDS             complement(17106..17645)
                     /locus_tag="JA154_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995324.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(17746..18426)
                     /locus_tag="JA154_05555"
     CDS             complement(17746..18426)
                     /locus_tag="JA154_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAT7995325.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(18455..19744)
                     /locus_tag="JA154_05560"
     CDS             complement(18455..19744)
                     /locus_tag="JA154_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995326.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            complement(20193..21794)
                     /locus_tag="JA154_05565"
     CDS             complement(20193..21794)
                     /locus_tag="JA154_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HAT7995327.1"
                     /translation="MIYDLILKNGFIIGGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIAKQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGINGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(21905..22582)
                     /locus_tag="JA154_05570"
     CDS             complement(21905..22582)
                     /locus_tag="JA154_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995328.1"
                     /translation="MSKVKSIYNEEYLPFKIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTMLIARAM
                     YKDKLSD"
     gene            complement(22595..23290)
                     /locus_tag="JA154_05575"
     CDS             complement(22595..23290)
                     /locus_tag="JA154_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 domain-containing protein"
                     /protein_id="HAT7995329.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(23342..24541)
                     /locus_tag="JA154_05580"
     CDS             complement(23342..24541)
                     /locus_tag="JA154_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAT7995330.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(24802..26847)
                     /locus_tag="JA154_05585"
     CDS             complement(24802..26847)
                     /locus_tag="JA154_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAT7995331.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
BASE COUNT         8713 a         4781 c         2746 g        10687 t
ORIGIN      
        1 tatataaaac tattttttat agattataaa atatctaact ttcatctact tcaaagaata
       61 caatacttct ccttctctta tagtctgcaa tactttaata tcttttattt tcatagggtc
      121 gattgtaagt gggttttcat ctaatattat caaattagct aatttccctt ctttaattga
      181 acctttttta tcttcttcaa aatattgata tgctgcattt atagtaacac cctttaaagc
      241 atctaatggg cttatctttt cattttctcc tatttgtata cctttttttg taattctatt
      301 gacagcacac catactgttt ccagcatatt aggtgctatt actggtgtat cctgatgaag
      361 agtgtaaatt aatccctttt ctatagttgt tttaagtggg ctaatcttaa acgctctatc
      421 ttctccaagg tttttgatat gaatatcacc ccaataatat gtgtgagcta caaaatatga
      481 aggtatcata tttattactt tcatatcatc aatctggtct gctcttactg tttgagcatg
      541 tatcataact ggtcttatat tattttcact actttgctct tttaagttta aaactttctc
      601 aaacgcatct atcaactggt ctgctgctgc atctccatta caatgagtta agagttgcat
      661 cttttctttt agtgaaatat ctataaactt ttctacttct tcatctttgt atataggata
      721 tccacaataa ccatcatctg aattttcata tggtctactc aaccatgctg tcttaccttg
      781 tggagaacca tctaagaaaa tcttataacc tcctatttta aatctattta tatatttttt
      841 tataaactcc ctattattat ctgctacaga cttacttttt ttcaaattta catatcctac
      901 aacatccatt tttaatttat tttgatttgc cattgctttt aaaatgttaa attgattttc
      961 ttccattaaa ccttcttgag ctgttgtaat accatagctt agatatatgt tctgtgcttt
     1021 ttcaattgaa ttaaaaatct catttgatga tggttgtttt attttagatg ctacattaaa
     1081 aaatgcattt tcttctaaat atccatttgg ttcttcactg ccttctactc tacctatatg
     1141 tccaccttct gggtcacgtg tttcattggt aacaccaagc tgttttaacc ccaatgtatt
     1201 tgcaactccc atatgaccag aagcatgtga aattagtatt ggattttctg aagatgcact
     1261 atcgagtaca ctcttatctg gatgcttatt ttcttctaaa aaattattat catatgaaaa
     1321 tccaattatc cagtctcctt tttttatatt attcgactct ttgaaatcct gtattttttt
     1381 tactatgtct ttaaaactgg ttgcatcttc taaaggaact agctttagag tagtagcaaa
     1441 agctatcaag tgactatgtg aatctataaa agatggcatc aatgtcttac cttgtaaatc
     1501 tataatttct gtatctttat ttttcaaagc aaatacttct tcttttgttc ctatcttttt
     1561 tattattttg tcttttataa gaatagcatc tccacatatt gaatcttcca ttgtgatgat
     1621 atttccattg taataaattt ttgctttcat aaaattctcc ttacgttgtc tactttaata
     1681 aatttttttc attattttct gcttaattat attatcttct ttataaactt tttctttaga
     1741 gtttaatcat ataaaagttt tatttttaat tattgtttgc caagaagtaa atactattca
     1801 ttgtaaataa gacaaattaa taggctttat actaattgaa aaaatattat aagttgttaa
     1861 atttaaatat ctaaactcaa ttatatctgt acaattgtaa ttttttaaat catgatgtat
     1921 tattttaaat atacagttac attattattc acaatataag tatcttagtt ctcaaataac
     1981 atctcatgta atctattcaa tgcaaaaaat ttaaatattt tttaggaggt tttttatgag
     2041 caaggaaaat aaaaatgtaa gaattgctgt tgtccaggcg tcaccagtca taatggattt
     2101 agaaaaaact gttgaaaaag cattaggatt aattaaagaa gctggtagaa aaggggcaaa
     2161 tatcgtagtt ttccctgaag cattcatacc tgcatatcca agaggtcttt catttggatt
     2221 tgttgttgga agtagaacaa tggaaggtag agaagattgg aaaagatact atgataattc
     2281 tgtaccagtt cctagtgcta caacagattt attaggaaaa gcagcacaag aagcaggagt
     2341 atatctatca atgggtataa cagaaagaga tggtaatgat ataaattgta ccctttattg
     2401 cacaaactta tttttctctc cagaaggtaa gctaattggt aaacacagaa aacttaaacc
     2461 tacaggaaca gaaagatgta tttggggaga aggagatgga agtactctta cagtagttga
     2521 tactccatat ggaaaaatgg gttcgttgat ttgttgggaa aattatatgc cattagctag
     2581 aacagctctt tatgctaagg gtgtaaaact ttacatagcc cctacttcag attcaagaga
     2641 agagtggcaa gcaactatga aacatattgc tttagaagga agatgttttg taattggatg
     2701 caatcaatat gttgaaaaaa atacgtaccc aactgacctt aactactata aggagctaga
     2761 tgcagaacca gaaattatgt gtccaggagg aagttgtata gttgaccctt ttggaaaata
     2821 tgtagcaggt cctattttca ataaagaaga aatgctaata gctgatttag acctagaaaa
     2881 aatagtactt agtagactag attttgattc agaaggtcac tattcaagac cagatgtatt
     2941 tgaattaatt gttcatgaat aatcttttta cacaaacatc tacataaatc tttttacata
     3001 aaaaaatgag atactcataa gtaaatttca agtatctcat ttttgcttaa aacctataat
     3061 cctaattata aatttattca cctatatttt ttctaaatca actactgttc ttccaacatg
     3121 ttttcctgcc attattgaat ctatactatc atttaactct tccaatgata ctacatttac
     3181 catatcatct aaatcatcta atttccattc atttgataat aaatcccata tttttaatct
     3241 taagttcatt gggcaatcca cagaatctat accatataaa gtaattccct ttaagataaa
     3301 tggatatact gaacttgtaa aatcaactcc tcctgcatta ccacaagttg ttactgctcc
     3361 acaataatta gttgtcttta ttgctgttgc tagagtattt cctcctactg tatctattac
     3421 tccagcccat cttggtctaa gcagaggtct tcctgagtta tcatccactt ctgacctatg
     3481 tataacatct ttaacaccta agtttagtaa tacttctttt tgttcttcta attttcctgt
     3541 tgaaactaca actgaataac ctaatttaat caatatttta gttgcaatta cagcaactcc
     3601 tccagtacca ccagtgacta atacatctcc catattcttg tctacagaat ctataaattt
     3661 atacacagat aaagctgctg taaaccctgc tgtaccataa tccattgctt cttttagtga
     3721 aagattttta ggaagtttaa ctacccaact agaaggaact cttatgtatt ctgaaaaacc
     3781 accagaagta ttcattccca agtcataacc tgttactaaa acttcttctc caattttaaa
     3841 atcatcacaa ctactagatt ctactatacc agatgcatca attcctggtg tatgaggata
     3901 ctttctcgta acacccttat taccatttgc agaaagtgca tccttgtaat ttaaagaaga
     3961 atattttact tttattaaaa cttcgccttc tggcaatgta tccacttctc tttcaactat
     4021 atttcttcta aaatttcctt cttcttctgt aatatatagt gatttaaatt ttttattcat
     4081 aaatttcctc ccaatacatt tagttttatg ttaaactaat tatagtttat aaatagtttt
     4141 atgtcaaact aatttaaata gattaaagat tggagaataa tttatggaag ataaatatat
     4201 tgtacatttc ataagtaaaa caaaggctag tatgattaaa ttcattgaaa ataaactcag
     4261 taaaaatggt ttaggtgagt taatacctac tcatggaaat attttaactg cactttatga
     4321 aaataatgga atacttacaa tgaaagaaat tgcagctaaa ataggaaaag ataaatccac
     4381 tgttacagtg ttggttaata aacttataaa cctaggatat ttagaaagac aaaaatgtac
     4441 aaatgacaaa cgtatcacgt atataaagct tacaaaaaag gcactgctta tagaagatac
     4501 ttttaatagc atctcaacac aagttaaaga gacggcatat cataatatta cagaaaatga
     4561 gaaacaagaa tttcttagaa ttttaaaaaa aattaatgat aattttaaaa atgctgacta
     4621 aattgagcat aattatatct cctattggaa taataaataa aaagaatcaa atattgtaaa
     4681 aatttattgt gatagagagg tagaatatga atttaaaaga tagtaaaact aaagaaaatt
     4741 tattaagagc ttttgcaggt gagagtcaag ctagaaatag atacaatatt tctgcatctg
     4801 tagcaaaaaa agaaggatta tatataatag agtctttatt taattacact gctgaccaag
     4861 aaaaatcaca tgcaaaacaa ttcttttcta aattaaaaga atttgctggt gaaaatataa
     4921 gtatatcagg agcataccct gtagatttat atgacaagac agcacaacac cttaaagctg
     4981 cacaacataa tgagcttcaa gagtgggatg atgtatataa aaactttgga aaaacagcac
     5041 gtgaagaagg atttgaatct atagctaagt tatttgaaaa tatagctgaa atagaaaaag
     5101 ttcatggtga tagatttggt agatatgcta ctgatttaga agaaggtact ttattcagaa
     5161 gaaatgaaga tatacaatgg atatgtacac attgtggcca tatacatata ggtaaagaag
     5221 ctccaaaagc ttgtcctgta tgtcttcatc cacaaggata tttcttagat tttaaaaact
     5281 cattatttga ataaattaag gcaataattc tatttaaaca atttactata tttatatcaa
     5341 gtatttttat aaatactgta ttatcttaat aacaataaaa tgaaccattg atattactat
     5401 aagtagaaaa tgtagtttaa atacaattta tagtaattca tcatggttca ttttattgtt
     5461 tactaatccc tataacctac ttaaatctat ctttaacttt tacatactca cataaaatct
     5521 tatctttaaa ctaaatattt aaataaagtg tattttaagt atattattat aatacattat
     5581 ataagataat acactatgat aactttatac ttatatcatt tatttctttt gtcaagtcaa
     5641 tctcaataat cttaactaca tctttttcaa actttacttt tttataatcc agacatccta
     5701 ctacgaatgg aactccaaac tttctaaaca aataaatctt tccactttca ttgtatattg
     5761 atgtaaaatt ttcttctatt atattcacta tcaattttaa ctcttcaaaa gttattatag
     5821 atttaactaa aaacttctct attatgctca atataagcag ttctttttta cctatgcttt
     5881 gctctttttt atataaattt tgaaatattt ctttggtata attacatata acttctttac
     5941 tgagaataaa atcaatgtca aattctttat taatatcctt tttcataaac atatttgcaa
     6001 tatcatcaag agaagtctct tctttcatag acaatattaa ctctattctt tctagtattt
     6061 tatctcttgg aaagaaagtc tcttgacctg taaaagctga tttttttata aaccattcct
     6121 ctggtattat gttttttctt ttccatctat ataattgacc ataagatata ttagttttgc
     6181 tcaataaatc tttcttagat attaattctt ccataacttt tctagcccac ctttatactg
     6241 ttttttacag tagaattatc tgaaactgat aaattatcac tgtattcaat atttccaaca
     6301 tcacaattat ctactagaga tatgctttta cctcgtatat cttttattga tgcatcttct
     6361 aattcaatag tatctccctc tatcaaatta catttaaact ttcctgaaaa aatattaatg
     6421 attttaatta atgaaaaagc atctttgctt cttcctattt ttatattagt tgcccctatc
     6481 tctttacaat aagaacctcc tcctgaataa acatatatat tttctccatt taaaagacca
     6541 tcacatttta ttgctccata tacctttata tcttcacatt ctatatttga tttatttctc
     6601 atttcgccat agatttttat atcttgtgca tacatatttt catttacact taattgtcct
     6661 cttattgtaa cattattaca tttaagtttg ccatccactt tctgttgagc agttataact
     6721 aagtcatcta tttctgcatc tccctctata ttaagttgac cattcactct tacacgctct
     6781 gctttaagat tgcctcttat aatagttttt ccgttaacaa caaaattttt tatattgatg
     6841 tcaccttcaa attttgcatc tccaagaact tttacttcgt tacttttaac atttcctttt
     6901 gatttagagc ttcccacgat ttttatattt tcatactcac cactacttat aaagccttct
     6961 ccaagtattt ttatagaccc tttttccata ttattacctc attttaaatt ttatttttaa
     7021 acctacttat tacttatcct tctttaattt tattttttca cttatattct ttatttcacc
     7081 aactattgaa tcttcatcaa tctctatact ttcactgtac tccaaacttt ctacataaca
     7141 tcccttgcct attttaatat tttttcctct tacaactttg acttcactat tttctaagct
     7201 tatttcatct gcttctataa tatttgcaaa caatttactt tctgtatact ttctaattga
     7261 taatagacct aaacttatca aatttgaaga cactttttca aagttgatat gctttttttc
     7321 tagcatacga aattttttgc aataatctcc atgccccttt tttatattta tactacttgc
     7381 tccaacttca ttaaaattag aagttccatt taaactaata ttaacttctt cacaattcaa
     7441 aaatccatta cactccaaga ttccacttaa attgatattt tcacaatcta tattctcatt
     7501 tgcttttaaa tatccttctc catgaataat atcagatttt atacttcctt ctacattaat
     7561 aattccacta gtatcaattt caacagaatt aatatttcct gtacatctaa gaagtcccga
     7621 tatctcaact tttgatgcca gtatgtttcc ttctacttta cttaaaccag atgtaaaaaa
     7681 ttcattacat tctgttgtcc caaaaacttt tgctgaacca gaagaatcaa atttactaca
     7741 ttttacactt ccttctattt tgcctgttcc tgatatatta acattatcaa aaattccacc
     7801 tttacatttt ccaacaccat caaatcttag tgaacttaat tcttcattca taatgatatc
     7861 ctttctttac cgtattttat ctttaaacct aatataacaa aacattgtta cgttgtcaag
     7921 tatgataata atacttaata aagcaactat catttatttt ctataaatag gcataaaaat
     7981 aatagggaaa tgctttattt aaactataaa tctaatttct tagattgtgg tttaaataaa
     8041 atatatccct attactcaat ctttatatat tcattgtata aacttctaaa atataagtct
     8101 ttaaattcta ctgtcaatac tttttataca ctgtttgtgc tatcatctca cacacctaaa
     8161 ggagtgggct ttccacacta ttctgtaacc tacaagacac tatcttcttc tatctattga
     8221 taaatttttc tattacatca gctagtctct ccatatcagt tggataaact tcacctatgc
     8281 tacctattct aaatgtatct atatcagtca atttacctgg atagataaca aatccattat
     8341 cttttaaata agtataaaat tccataaatt caaactttgc atttttagga tataagaaag
     8401 ttgttattac aggtgattga gcattatcat tcaccaaagt atcaaatcct aaagatttta
     8461 atcttgaagc tattgtaaac tgattttctt tatatctagc atatcttttt tcaacagaac
     8521 cttcttcttc caattctaat aaagcttgat aaaaagctct tacaacatgt gtaggtgagg
     8581 taaatctcca tttaccatta tttttctcca tagtctccca ttgcgcatac acatcaagag
     8641 ataatgattt agctatacct ttacatttag ataatttctc tttattagct attataaaac
     8701 caaatccagg tactccttgt atacatttgt tagaactact aactaaaaaa tctatattaa
     8761 aatcttcaac atcaatctca attcccccaa aactactcat agcatcaact atataaattt
     8821 tgttatattc ttttgcaagt ttccctacct cttgtatagg atttaatcta cctgtagtag
     8881 tttcacagtg aaccattgat atatgtgtta tatccttgtt ttcttttaat aaattttcta
     8941 ctacatttaa atccactgct tctatatctt taaaagtgca gtcaacaaat tgtatgttaa
     9001 gataattaca tatatctttc attctctttc cgtatgctcc atttgcaatt acaagaagtt
     9061 taccatcttt tgaaatagta cttcctatta tagcttctac tgaaaaagtc ccacttcctt
     9121 gcattaatac acttgtgtat ttatctgtat tttgggttgc tagagatact aatcttcttc
     9181 taacatcttg tactaggtta ttatattcaa catcccaagt acaccaatct tttaacatag
     9241 atgctcttac tgatttagta gtagatagtg gtcctggtgt aagtaatatg taatcatttt
     9301 ctggtacaaa atctgattta atagtttcat tctctatttt tattagtata tcttctaact
     9361 cagccatagt ttctattaca aaatgtgctc ctgcctcttt aaatttcttt gaaactatac
     9421 tcatcttagc ttttaattct tccttatcca tgttctctac ttcttcttgt gtaagcccta
     9481 actcacttga accttttatt acagcaacac tccacattcc tgcatttacg ccttctttta
     9541 catcacttat tgtatctcct acttttacca tagaactcat aggagacacc cctaaagctt
     9601 cagcattttt atagcacatc caaggatatg gtcttccttg agatacttct gatggtgtta
     9661 caagaaaatc gggtgagtat cctttctttg ctgcatttgg ttcaactatg ttcatcatct
     9721 ctcttgtata acccgttgta gaacctattt ttaatccatt ttttctcaac ttttcaatag
     9781 tttcaacaac atgaggaatt ggagtcgtat actcttctaa tgtctcaaat aacattggct
     9841 caaattcagc ataaagttca tttacatcat cttcagttgg cactttacca aacttatctg
     9901 accaaatatt ctttattcta tccatttcac acatttctct tatatggtct atttttaatt
     9961 ttcccatagg ttttcttgct tcttccattg ttacatctat acctcttctt ttaaatatct
    10021 ctataaatac attaagaggt gcaaaacatc cataatctac tgttgttcca gcccaatcga
    10081 atactactgc ttttattttt tctccatata tctttttcat atttatagcc tccatagatt
    10141 ctttttagaa tttttgtgtt tttaattgtt tttactttta aagtataact ttcatatgtt
    10201 aattatacat catatttagg ttaaatatat attaaatttc tgtttttagt tgtatttagt
    10261 tgtttttatt ttgtatcata ttcccataaa atcctaagtt acaatctaaa atttactttc
    10321 taatcttaat ctatgaactg actatatttt atgaatttag ttgtattgag catagttgta
    10381 ttatataaaa ctaagggttt attttgcttc tatccatgtt atatttatct catttaactt
    10441 atccttccat tcattcggag gttctacatc acaaataaca aaatctaatt cctctaaatc
    10501 tgctatctta tacatactag caattcctat tttagaacta tcacataata caaaactttc
    10561 ctttgaattt tttatatatg cttttgacaa caatgcctta ttttggtcta tactactaat
    10621 tccaaaacta tcaagtatac cttcactaga tataaaagct ttatctacgt ataagttttt
    10681 catagcatct attgatattg gacatgctgt cctttggtgt tttaaattta cttttccacc
    10741 aataaatata acttctccat caaacaaacc ttgattctcg taagaaatca attttgacag
    10801 aactggataa gagtttgtaa taatttttaa attgtttaca gatattatat aatctacaat
    10861 ctgcaaattg gtagtacctt catctatgat aattgactca ttgtcattta caaacttagc
    10921 tgcatattta gcaatacttt gctttttatc taggttagta ttgtttctct ttatatgttc
    10981 tggttcaatc actccataat ttatttttat agcaccacca tatacctttt ttaatttatt
    11041 ttcattttct aattcttcca aatatcttct tattgtttct gatgatactt ctaatctttt
    11101 tactaattct ttagtgttta ccttcccttt tgaatctagt aatttaagta tttctttttt
    11161 tctatctatc gaatttaaag acattcattt tcctcctaaa tataatatat ttttctccta
    11221 taatattaca atgataccta agatttatga aatcatattt atgtattaaa ttttttactt
    11281 aattaaagtt attttcaaaa gactttgtat tatcattaaa aaagtccatc aaatattagt
    11341 atcagatgga ctttgtaaaa ttttatttta taaattaata tttaaagtct aactactaat
    11401 ttattttctt gtctccaaga attttactaa attatttaca gtagacatat cttttctctc
    11461 taaatcagtt ggttgaagct ctatccctaa tctattttca atttcaagta aaacttcaat
    11521 tatagctaat gagtctaaaa gttccgtctc aaataaatta aggtctaaat cttctcttat
    11581 ctcatcagtc cctaatacat cttcaaatat ttctataact gtttcttgca taaattttcc
    11641 tccttaaaat taagctaaat aacctgagaa tatcagcaat ccaaaacaca caatatggaa
    11701 tgtaatgaaa atctgcactc tctcgaacca tttatctttt ttatgttttt tataaaattt
    11761 agattttcgc tgatatatat cagttaacac aagtgccaat ccttggtata caccataagc
    11821 aacataatac caagttagac catgccaaaa tcccattgtt atcatggtta tcatctgagc
    11881 cacatgagca gcagtagtcc ttttcttaaa tcttttcttt ctcattgatg acataacaaa
    11941 tctagaaaaa atataatctc caaaccatct tgaaagactt atatgccatc ttgtccagaa
    12001 ctctttcata tctttgctga taaatggctt gtcaaagttt ataggtactt gtattccaaa
    12061 tatgtaccct gtacctatag caaataaact atatcctgca aaatcaaaga acaggtataa
    12121 actatatgcg tacatataac ttaaaatatt tataaaactc atatctttag gtattcttga
    12181 tacccaatat gtatttatca aaaatgctat aacaaactta taacctactc ccatgacaat
    12241 attttttatt cctggcagta agtattcttc tatatactct tttctagaga tttgtttttc
    12301 caagtcctgt tcaaatcttc ttgacctatc aataggtcca gaacttaaag ttggaaaaaa
    12361 taacataaaa taaagcatct ttgaaatttt aacttctttt atagctccat cataaatttc
    12421 aataaccatt tgtatagttc tgaaatttaa gtatgatatt ccaataaatc caattattcc
    12481 aaaagaagta actggagata ttttatttat tattattggg agcattgatg caaacaagaa
    12541 tccccaatat atgtatttat ttttagtttt tcttcttaca tattcatatc ctttaactat
    12601 tattacttca agaataataa atattaccaa gtactttagt tggacatcaa ttcctactat
    12661 taagaatatc ataaacaaac ttgccaacat accataatac tttatattct tgcccattag
    12721 acctaaaatt actgctggta ttgatgttaa taataatata taaagataaa aataatctcc
    12781 atattgtgaa aaaatcatta aatttcctcc ataagtttct ttctgtctat tttaccattg
    12841 atatttgttg gaaactcaga gattattgaa atatttcttg gaatcatata tgaaggtata
    12901 tactttccaa gttccttttt tattatcatc ccatttttaa tattacttaa atcattcttt
    12961 tcatttaact caacaatacc ttttaagtaa gcaatttttt cgtccttata tactggtaaa
    13021 actactgcat tttttacatt atgtactttt ctcaaattat tttctatatc ttctatttca
    13081 attctaaatc catttaattt aatctgaaaa tcttttcttc cacaataata tatatttcca
    13141 tctaaaagat atcccatatc tccagtttta taagctctcc actttacacc atctatttca
    13201 tcatagaaaa atacttcatc agtcttttct ttattattga agtaaccttt agaaactgat
    13261 ggtccaatta ttataatttc acccttttca ttttctttaa gctcatttcc atcttcatca
    13321 agtattttta ttttacaatt tgacataggg tatccaactg gaagactttt ttcatcatct
    13381 atagcctttt gagtcatatc atttacactt acaccaacag tagcctcggt tggtccatat
    13441 ccattgataa ttcttgtgtt tggaaatcta ctcataagtt ctttagtaag attttttgaa
    13501 agtgcttctc ctatgaatat cattgactca aggttaggaa gcattttact attaaactct
    13561 ttttcagtta tacatactcc tgcaaaagaa ggagttgaca cccatacagc tatatctgag
    13621 atactaaatt gtctaaatag ttctttatag tctgctaata catcttttga tattgaaaat
    13681 aatgtagctc catggataag accagggtat attgttgtaa ctgaaaggtc aaaagaatat
    13741 gctggttggt tcattattac tttttcactt ccatctatgt ttagatatgg gcttatccaa
    13801 tctgaaaagc tatctaaatt gtttgaactt atttgaaccc cttttggctt tcctgtactt
    13861 cctgaagtaa atagtatata tgcattttca tcatctttta cccagttttc tttatctaat
    13921 gattttccct gatattcatt tattatataa tttaatttat ccatatcaat tacattgata
    13981 tctccaaaat ttctttcatc actaaagtta aataatacct ttggttttat ctctttagtg
    14041 acttcaaaaa ctctatcaat tggaaaacta atatcaagag gcacatacgc tctacctgat
    14101 tttaaagctc ctatcataca tgccattatc atgttttctt tatttccata aattactata
    14161 ggagtatctt cttctttgta tacatccttt agaaatactg atatagcatc tgaatattca
    14221 tttaaatctt tataagaaag tttatctcca ttacacatta gtgctgttct atctgtattt
    14281 gaatattttt ttattccttc aataattttc atataagaat gctcctttaa aatccgttat
    14341 aaataaatgg caattcttta aatggagtaa atgttagaac tcctatcgcc aatattataa
    14401 ccatagttaa acaaatacta gttaacaaat ctttattgtt ctttaaatat tttaaataat
    14461 ctctcacaga catctaccca tcctttcgta ccaagatgca ttacatccct taaataatac
    14521 ttatctgacc ctttatcttt aaggtttaga acttcaaagc ctttgctttc tgctattttt
    14581 tgtgctttat cgtagtactg atttctttct ttttcagaaa taccagtcaa attatagaat
    14641 ttatccatac ttgggattaa tacaacaact ggcttaatac ctaagtcagt acatacattt
    14701 agcatcagtt tataattttc aaactcctta gatgttaata aatttacatc tttgtctctt
    14761 cctttgtatt gctctatacc atctttaaaa tgctctttat aatagtactt atcaatattt
    14821 agtggatttt taccaactct ttttttagca tcttcaatgg ctttctttct ctcatatgac
    14881 caatttattg gtctttttct ttttgttgct ctctttttat ctagcttaat taatcgttta
    14941 taaagaatac ccttttcttt aagagctata caatactttc ttccttgaaa ataaggctct
    15001 aaaagcacct tttcagcttt ttcaggaaat gtcttaggct catatagctt agcatataat
    15061 gcttctgctt tatactcgtc acttccccat aataacttac ttgatttttt tgcatattca
    15121 atcttatttt gtttagatat ttttggatta tctaaaaatc gataaaattg tattggtgaa
    15181 aatctagact gataatggtg acttgtgact ccgtcttttt ccataaacca ttgcatagaa
    15241 attaacaaga caactttctt attatcaata ttaggattca tacttcctaa tattgcagca
    15301 tcttgtaatg tctgagtata ggctctccca attgcaaaca ccttattttt actcctattt
    15361 gtattaaaat agtactttgg atgttgtttt gttgaattac taagttctga tgaccccaaa
    15421 atcataatat ccttttctct taaaaaatga ttatttgctg taaccccttt atcttttata
    15481 tcacttaaag tatcatccat aattggaagt agatttttct ccctcaaaag ttcatcagtt
    15541 ttagaatcaa gaaatttgtc taacccaaat aaaaatacta cgcctattat aaaaggtgta
    15601 ataaagtata tcaattttct catattttct ctcctctaaa aatattcaaa tttatgttat
    15661 tattaatcat attagttttg atatgtatat tcaactacaa aattgtaata aaatcgtcaa
    15721 aacttttttt gatactgtta caaaattgta aaaaaaaaga aaaaaacgcc aaaaataaat
    15781 ttctttttag cactttttca tacttataat ttgacatttt aacacttggt taagtataca
    15841 ccttacctta acttaaaatt aaatttattt aagaatatat attataaatt cttatatttt
    15901 ctaatataaa ctatatacta tcagctggat aaacaatact actttgtctt cttatctcat
    15961 ccataatttc cattactatt attgaatttt catgagaatt tatatttgat tctattttat
    16021 tgttatttat aagacttata aactctacaa gttcataata cattccttct atttctctag
    16081 gcttttcctc tgctttttct tttggaatag atatatcttc ttctcttcca tctctgtatt
    16141 taataatagc tttttcaaat agattaagct tttcaattat tatacttccc tcttctccct
    16201 gtatttcaga tggtatataa gaatcagcta ctttagaata ctgaattatc gcatccatat
    16261 catcatattg aaatacagct gaaccttctc catccactcc agtttgaagt atatgagaaa
    16321 ctgcctttac agtttttgga gcaccaaata aattcaccat aggatgtata caatacactc
    16381 ctatatccat taaagctcca tttgacaact cttttttaaa ggcattttct ataattccat
    16441 ttttatattt atcatatctt gaagaatact gacaatagct tccaaaaaat cttcttattt
    16501 tccctatttt atacaagttt tctttaactg ctttaaagtt tggaacacat gtaattctca
    16561 tagcttccat aagcaataca ttattatctt tggctgtttt tatcattgat ttgacttctt
    16621 ttaaattaga tgctaacgac ttttcacaaa gcacatgttt tttgtgtttt aaacaagtaa
    16681 tggtttgctt tgaatgtaat gcatttggag aagctatgta tacagcatct atatccttcg
    16741 atttagccat ttgttctaaa tcatcaaaaa aaatacttgc tccatataga gttccaaatt
    16801 ctctagcttt ttctaaattt ctagaatata cagcaaccaa ttcaaagtct ttaaccctag
    16861 atgcagctct tagaaatata tttgctatat tgcttgtccc aacaatacca aatcttattt
    16921 tacccatcat aaatcactcc ccattttcta cactttattt taattttaat aaaaaacttt
    16981 acaacttata gatatattat aaaataattt tatcaaataa aaaagaaagc taattttaaa
    17041 tgatttcacc attttaacat agctttcttt taactacaat ctaaattcaa cttatatttt
    17101 taaagttatc tttctactat atctttattt cccctcaaaa attttatagt aagaatttca
    17161 tccacaaaca atgttactaa aagtatagtt acaacaattg gccaataagg tattataaga
    17221 ctatataaac tatcaactat atacacttga aaatatctaa ctccaataaa tgtcaatata
    17281 gtccaatata cagaaaactt tagacatact attcctctac tattgtactt ataatctgaa
    17341 taatcccaat agaccttatt aaaatatact ttcataagta ttcctgtaat atattctact
    17401 actgtcggca ctactagaca taataaaagc aatgcaaata tattattggg gtcaataaat
    17461 cttgagacct ctattataat cgacattgca attgcataca ttggtttaaa tggaccattt
    17521 aaaaatccat cttcttgaaa atgcccttgt ttataataac tatataagtt ttcaattatc
    17581 caacctataa aaccatacaa gaaaaaatta aataaaaaat ataatagaga acctacatta
    17641 tccatgaata ttcctcctag ctgtttatac ttattttttc tcatattcat ggatttattc
    17701 taaatcaaag aaattatatt taatttattt aattaatact ttatattata gattgtgaca
    17761 ttctttagag ttcaaacttt ttaggtattc tctaacttcc agtaaatttt caagtatctt
    17821 atgtgattgt gatttaattt gctcatctgg ctcttttaaa tctggtacat ttatacatct
    17881 tataccacca ttgtatgcag cttcaactcc cataggcgaa tcctctatga caatacaatt
    17941 tttgggatta acatttatct ttttagctgc ttttaggaat atctcagggt ttggtttcga
    18001 gtttacaaca tcatctccac aaactatagc atcaaagtaa tcttttaaat ttgccttagc
    18061 taatctcttt actgctcttt ctctctttgt tgatgttgca acagccattt tgtatccatt
    18121 ttcctttaaa aaagagatta gttcattgac tcctaatttg ataggagctc ccttcctctc
    18181 cataaattca atcatatttt tagttttttc gtcatataaa tctattattg gtacagaact
    18241 atcgtatata tctgtaagac cttctattat tcctttacga tttcttccca taactgatgt
    18301 ataaatttcc ttagtcatag tatatccata cttttcaaac gtctccatcc aaaattcaag
    18361 agaaattctc tctgagtcaa acaaaactcc atccatgtca aaaataattc cttctacttt
    18421 ttgcatacaa ttcctccaat attttttgat ataattatat tacacttttt atgattaatt
    18481 ttacaaccct caatttatcc aattctaata ttgtaaaatt gtaattttca aaagttacac
    18541 tttggtttac tttaatattt gaaccaagca tataggatat ccatccacct atactgtcaa
    18601 atccatcatg ctctatatca agtccaaaat acttattaat atagttgata gattcagttc
    18661 catcaactaa gtaagaacct gctcctattt tcttaatatg agtctcttct ttatcaaatt
    18721 catcttgaat ttctccaact atctcttcta gtatatcttc tattgttacc acaccagatg
    18781 taccaccata ttcatccaca actatagcaa gttgaagttt ttcctttctt attctctcaa
    18841 gagctttatc tatagttaaa ttttcagata tatatataat atctctcaat atttcttcta
    18901 gttcaatttt attttcattt atcttttgat tgtataaatc tcttatatgt acaaaaccta
    18961 atatatcatc tttatttttt ctgcatactg gatatcttgt aacaccttcc tcttttaaaa
    19021 tagctaatat cttttcttca gaatcacttt catatataca taccatatca gttctaggaa
    19081 ccattatctc tctaattttc ttttcttcaa attcaaaaat attatcaact aatctttgct
    19141 ctgattcatc tataagtcca cttttataac tttcttctac taatagtctt atttcgtcac
    19201 ctgtatgggg ttcatcaacc tcatcagctt gtgaatatcc aaatggttta agaaataaat
    19261 cagtacttaa gttgaaaaaa tatattattg gatatgttaa cttataaaat cctactaata
    19321 aaaaagatgt actcaacatt attttttctg tattataaag tgctaaagct ttaggaacca
    19381 actctcctat tacaacctcc atcattgtta taattaaaaa agaaatggaa attgatatag
    19441 tatatataat actttcacta agattaaata cacttactat tggtaaaatt aactctttaa
    19501 ctgtagattc tcccatccaa cctaatgcaa gtgaacaaag tgttatacca agttgacatg
    19561 cagaaagata ggaatttaag ttgtctttta ttattttaca tctttttgca ttattattac
    19621 cttcagttat catggtatca atcctagaac tacgaagttt taccatagca aattcacaag
    19681 ctacaaaaaa agcatttatc aaaacaagca ttactataag tgttactttt aataactccc
    19741 gcataaagta taggttctta taatgtaaga aatctataag aactcaaagt tcccccctta
    19801 tattaataaa tttaatttat aatatatatt ctatcatatt tattaatata tcctacttta
    19861 ttacaaataa tttttataaa ctaaataacc ccttttaata tttatgactg gctatttgat
    19921 aaatattaaa ggggttattt agttatcatt ttttatttct aaaaatcttt tctcaaatga
    19981 tttagttatc tatttatcca attctttaca taatttaatg attaaaaata ttaatttttt
    20041 cactataaag tataaatatt atctgattat taaaataaat attaatacta taaataaaaa
    20101 cgaagcatga tttttattta aataaatctc aaacttcgtc tttacttact tagatattta
    20161 tttactttct aaatcctatt atttttaaaa ccttatcttt ttaatacttc accacattta
    20221 ttctcagtaa tctctccatt ttccaacgca atttttccat ttacaattac atattcaaca
    20281 ccattggcta gtaattgtgg ctttgaaaaa tcaatatctt ctgacctaga ataactatag
    20341 ttatccatgt caataactgc tatatcagct atcaaccctt ctttaagaat acctctattt
    20401 tttattttaa attgcctagc tggtaatgaa gtaactcttc ttacaatctc ttccaatcta
    20461 acaccagact ttctgtacat attgaagaaa attggaaaag ctcctcttct ttgtagttca
    20521 aaccatgaca tatccccacc cacactaaac aaacaatctg aaccaaccat aacaaatgga
    20581 ttatcaatta ttttatcatc atgtagttta tctgggaaat cagctctata cattcctcct
    20641 aaacaaaaga taagttcttc gtctccatct aaaagtaaat cgagtaataa cttgtcttca
    20701 tctattcctt tttgtgtagc tatttcacgt attgatttct tttccatatt aatatcttta
    20761 gtgtttagaa gtattatact ccccctatct ccaagtataa atgcttcttt taatataagt
    20821 gttcttccct cttcatttct aagagcatcc attaccccat ttatacctaa ttcaaataga
    20881 ctagatgata ttgccataat aaattgaatt actctttttt tcttcataca atgcccagaa
    20941 cttcttggta ctgtgtcaac cattatttca acaccattat acctagcctt taatatctca
    21001 tcaaatgctt ctatttcaac tggacttaag tgagatactt gaaccctagc accacttttt
    21061 tgcccaacct ctatggcttc ttttactgca ttatatcttc ctatgtaatc tctaatatga
    21121 gatgtatata taccatcatg ctctttaatc tgctttgcaa tatcaacaag ttcatcaata
    21181 tcagcatact tacttggtat atatgccaat ccagtagata ttccaaaagc accacttttc
    21241 attccatcat ttattatatc tgtaatttca tttttttcct tttctgtagg aggtctatcc
    21301 ttagaacctc ccataacact ccatcttata gtcccatgcc ccaacaagaa tcccatattg
    21361 acagaaatac cacttttaga tataaggtca caatattctg taaaattatt ccaacattta
    21421 tttttatcta taagatactt tttttcttct tcaagtaata gaccattttt atacatatat
    21481 tcatatacat tttcagaaga atatggtgtt atagaatgtc cacaattacc atttatagtt
    21541 gttgtaactc cttgttttag aaacttttca aatttaccat ctagtattgc tactatttct
    21601 tcatgaacat gagggtcaat aaatccaggt gttataactt ttccacaaca atcaatctct
    21661 ttatttgtat tgctatttat cttagaatct atttttgcta ttaagttgtc tttaattgct
    21721 atatctccat aaaaaccagg attacctgta ccacctatta taaatccatt ttttaagatt
    21781 aaatcatata tcaaaatatt ccctccttaa aaaccaattt ttaaataatc ttaatgtaat
    21841 ttttatattt gaaaatcaaa ttattatatt tcatatttga aaattcacta ttatttcatt
    21901 gtaattagtc actaagctta tctttataca tagctcttgc aattaacata gtaccaaata
    21961 cacatataag aactactaga gactgtggta ttataccttg ctttgataat ataatagtca
    22021 atacagatat tacaattgct actaggccta attttttatc ttgtaaaaat acttgtccaa
    22081 aaactgaccc aaatagagct ggtaaaatta agtttaattt ttctactact tcagctggaa
    22141 ttttagatag tacgcttcct cctaaaatta cccctatagt tagtatactt atattcacta
    22201 aaatagatac agctattcct attgtagata ttatcgagcc ttcttctgta cctgattcaa
    22261 cttctgctgc cttttgagct gctattgaac atggtagcct catattagat atattaccag
    22321 aaagaaaact catataagtt cctgggatac ctaagattgg tgaataagaa actggttcta
    22381 ttatgtaatt tggagcactc atactgacaa ttgccattgt acttgctaat aatgctgata
    22441 ttggaggcat aataccaaat ccaaagctca aaattattcc aggaactaat gctgctataa
    22501 tccccaaaga tagtgttagt cttccatacc ttatcttaaa aggtaaatat tcttcattgt
    22561 atatagattt tactttactc ataaaactcc ccccttatat tactgaagct attatcattc
    22621 cgctaaacat tgatattgta agcccccact ctcttaacca ttttatattt ttcttctttt
    22681 caagcataca caatattacc attatagcca ttccacttat acatgcaatt gttccattat
    22741 caaaacgcaa caccctatca gcatttagat atgcaaatga acccaacatt gctcctagag
    22801 atattattgg tataaatgat ttcttgccac tagatataac attggttatc ttatctagtt
    22861 tatgtccaaa tagtaatgtg aatattatcc atcctaaaga acctaatatc atagtccaaa
    22921 ctgcatttgt aaatgcaagt tttgtcattt caggagaacc taaagttata cctaatgcat
    22981 ctgttccaaa tccggctgac attgattcaa aaattacaga acctataaat gataatctca
    23041 tccatgctat aggcccaccc atagttacta taagcgatac cattccagca aatatagtca
    23101 ttgaaggccc aatagctgaa atagcactac tcttaacagc tgactttatt tgtttatctg
    23161 ttattcccat tgttttacca actttgcaag attttatagc aaatataagg gattgtacca
    23221 ctactattga tactgctact gtactcgcta tccacaaaag tggatgatta gctaatttta
    23281 aataatccat ttataaaacc tccctataaa tcctcaaatt ttattaatat ttctaatata
    23341 tctatttcca aacctgtttt agtactctaa gtatatttcc acctagtacc ttctctattt
    23401 cctcatcact atatccatgc tttactaaat atctaacaat gttttttgaa gtttctgttg
    23461 ggttctcaag accttttaca tattctactt tgggatgttc ttttgttcct gttatttttt
    23521 ttatatcaaa tttagcagct agtactgagt gtaattttac atggtcgcca tatactgtat
    23581 ctggtccaaa agctacatgg tctattccta ctaaattttt aatatactca aaatgttcca
    23641 taaatgactc tattgaatgt tctttattct tctctgttat tgtagtatga ggtgctgctt
    23701 ctacacctat aacccctcct tttttagcac atgctattaa tacttcatct ggagttagtc
    23761 tctttgtgtt ccataaactt cttgccccaa catggcttaa tattattggg tccttgctat
    23821 attctattac atcaagtgta gtttgaactc caacgtgaga gcaatctatt gccattccaa
    23881 ttttattcat tctttcaaca gctttttttc cgaaatttgt aagacctcca tctttctctt
    23941 cttttagtcc tgaacctaat gcattggatt cgctatatgt tatacccatt aaccttatac
    24001 caaatccata cagtatatct attctatcaa gttcattttc tattggtgct gcaccttcta
    24061 atgttggtat caatgctact ttaccttcac ttttagctct gtatatatct tctacttttt
    24121 cgcactttat aacaaaatct tgatgagcta aatcacatag tctcatgcct aaatctatta
    24181 atatatcatc ccatttccac cctgaatttg atgttatcgt acatacacca tccattaaat
    24241 tatcaaatat agcatcataa tatcctcttg ataatgcttc ataagcacat ctttgtttac
    24301 cttccctatt atattcaaat atatctcttt ctaggtgttc tggaaataat actggatgtt
    24361 catgtaatga tataaatatt ttttcatcca ctatactttt aaccttagct tcttgaatat
    24421 catctaattc aataaggtat tctttaactc ttttatctcc tttacacatc tcaatagatg
    24481 aaatatcttt tcctttttct aaataactat aggcttcata tcctttgtaa gctgacttca
    24541 taacaacatc ctccaagttc tacaattttt tatatttttc ctatgtagca attgatgtgc
    24601 caaaatataa attttaattg tataataaat cttgcaaaaa ataaaaacag tagtaaggtt
    24661 gatatttact actgttttat taaaaataat cttcttagat tatttattca atatttttga
    24721 attatcttaa tcttaaagat aattttattt atttctatcc attataggga tttttttagg
    24781 gtgttttgcc tttttagtcc cctatatcac tcccttaatt tttacatatg atataaagtt
    24841 ctttccttca tcagttataa aactaccttg tttttttact ccagataaaa gtaaaccatg
    24901 gctctttaac ttatctattc tttttctaat ttggtctaca cttaaaacta tatctttttc
    24961 ttttaaaatc tcttgcagtt tatttcttcc taataatata tttttattat tccaagtttc
    25021 tatactggtc aatatacata tactttcctc aaagaaatta gattgtttga aatctaagat
    25081 aattgaatcg aaattttcat taactaaagt attgtttttt tcaaatctaa attgttctgg
    25141 caaatgctcg taatctactc tatcttcctc aactatagta tcaatataat aaattaaatt
    25201 tttaagctct cttacatttc ctggccattc atacaatgtt aaagcttgca ttgattcctc
    25261 tgtaaaacat ttgttgctat ttatttcatc taaaaaatac ttacttataa gaggtatatc
    25321 ttccttacgt tctctaagtc taggtatctc tatatgtaat acattaatcc tataatacaa
    25381 atcttctcta aaagaacctt ctttaatttt tttctttaaa tctttatttg ttgcagctat
    25441 aattctaaca tctattggta taattttact tccgcccatt ctcataactt ctttttcctg
    25501 taatactctt agcaatctct gctgtacatc taaagatata tcacctatct catctaaaaa
    25561 aattgttcct gtatgtgctc tttcaaatat acccattttt ccacctttta ttgctcctgt
    25621 aaaacttcct tcatcatagc caaaaagttc actttcaata agtgtatctg acaaagaaga
    25681 tagattaact gcaacaaatg gcttgttttt tcttagagat tcattatgta ttgcctgggc
    25741 aaatatttct tttcctgttc cattttctcc aagtatcaac acagaaaagt ctgtagttgc
    25801 tatttttctt gcaatattaa ttttttcttt tattatttta ctctcaccta caacactctc
    25861 aaatgtatac tttgatacaa aaccttttga ctgaagttta ttttgaatct tgtcctcaag
    25921 cacttgaatt gcacttatat ttttaatgct tataatactc tttattcgtt cattattttc
    25981 ataaacattt actttattaa ttatcagttt tttgttattt aaatttacta cttcatcatt
    26041 aacttcatct tcttgaaaaa atattttttt gacaacctta tcactaaata agtccataaa
    26101 gttattagat ataatttcat tttggtctat tccaactaga ttagaaaata ctttgttgca
    26161 atatataaat tttcctaatt tatcaacaga agcaatacct tcatctataa tctccaaaaa
    26221 actcttcatg gttttattca tagttgtata atacctatat ccacttactg tatcttcatt
    26281 gtatttttcc ttaataatat gtagtttatc tatagatata tttagttttg taaagatttc
    26341 tataactgta tttatatcaa ttaccttatc acctatgtca attatttgct ttatgttttg
    26401 tggtatgctg tttctgcttc ccgtaataat tccaatctca catttagatt tatcacaacc
    26461 tggataatat ggtattagat taatatgaat taatcctagt tttctaatta tcttggcagt
    26521 ttcatctgct gattctttat aagcatctat aaccataaca tcagaatcat tttctatact
    26581 tattatctga tttatatttt caatatttat agtacgatgt actattacaa taggtatatt
    26641 tttatcaata ttattatgta tatattcctt tatatcattg tctgaacata ctacaagctc
    26701 atagcttgac agttcaatat ttatcctatt tacatatacc aagttatcta tattacaaaa
    26761 ttctccaaat atattttcca attgttttga tatatctata tttattttgt catcagttga
    26821 tacaaataaa atattatgtt ttttcatagt cccacctcct gtttaacgag tatagaaagt
    26881 aataatttgg tctattgtag cactttttaa aaaagtttta aatctta
//
LOCUS       DACULQ010000031        26809 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.031, whole genome shotgun sequence.
ACCESSION   DACULQ010000031 DACULQ010000000
VERSION     DACULQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26809
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.031"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            319..2214
                     /locus_tag="JA154_05590"
     CDS             319..2214
                     /locus_tag="JA154_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HAT7995332.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            2676..4079
                     /locus_tag="JA154_05595"
     CDS             2676..4079
                     /locus_tag="JA154_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HAT7995333.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     regulatory      4251..4336
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4460..10423
                     /locus_tag="JA154_05600"
     CDS             4460..10423
                     /locus_tag="JA154_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HAT7995334.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDGLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQEISKGNSDLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGIINNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     gene            10627..12507
                     /locus_tag="JA154_05605"
     CDS             10627..12507
                     /locus_tag="JA154_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HAT7995335.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            12874..14475
                     /locus_tag="JA154_05610"
     CDS             12874..14475
                     /locus_tag="JA154_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HAT7995336.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASNSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLLVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            14694..16292
                     /locus_tag="JA154_05615"
     CDS             14694..16292
                     /locus_tag="JA154_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HAT7995337.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTIILVNTDNSIVDGLSASGLSGVAKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNDLKANIKGNVDINKAGTYVLNISVVDNLGQKSEISVNVKVVVNASTKDSNS
                     YEFKAMVSNEMYDLVNSYRKEKGKKSLRELDSLAGMANAWSKYMEDKKVFAHEIDGKN
                     AAEVFFGFGARSGENIAYLPMNVKSVYTSKDAKEMAESIFDLWKKSSKYNENMLKEEF
                     YSFGFGMHVSSKGEVNATMEFLNS"
     gene            16582..18780
                     /locus_tag="JA154_05620"
     CDS             16582..18780
                     /locus_tag="JA154_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT7995338.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYTVVQDKYEKLL
                     KELKAKIKDGTITSVGVEFDGKPITTLAPKADGSDKDAIAEQLETLTKNQLKGLGDGK
                     YVDFKITYGAKAEVPAASLSADDIQKYADQINASEKILVEVAAGSEAGIAKFDSVNNK
                     VIAGDAPLKVKDAVKATVTTNGSNKKVLTISAAAGLSGFSYGTLKDKGASSSDVDAIT
                     LDTTNATITEGDTKVLDFDNSFKFNESTKKVGSLVTPNTTNTPADPGTKTTVRVIKAV
                     EKTIDVSSNSTTKAKDLAKQYVFTDVSDTDPESLSYMLKNINDGKVAVKNSDGDYEVT
                     IFPEGKRLNTLSASSAKTILGDKDTPAKIVLKASTTKKLADYIDDLITYNNSYSNVQT
                     VAGSDRIETAIELSRKYYNSTDKNALYGDPVNNVVLVGSQAIVDGLVASPLAAEKDAP
                     LLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGLK
                     VTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVVV
                     DGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATNA
                     EVIKEYFDKDGVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNEDGDITTDASPA
                     PIILATDNLSAEQHVAVSKTATTNGAKNLVQVGQGIADSVVSKLKDLLDM"
     gene            19026..21368
                     /gene="secA"
                     /locus_tag="JA154_05625"
     CDS             19026..21368
                     /gene="secA"
                     /locus_tag="JA154_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089305.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAT7995339.1"
                     /translation="MSVLDVILDKTDELEIKKLNNIVDKIDALEDKIQILDDEALKNM
                     TNVFKDKLSKGETLDDILPEAFAVVREVSKRILGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPVYLNALTGKGVHVITVNDYLAQRDKELMQPVYEFLGLTVGVIINGQQ
                     PVERKLQYQCDITYGTNNEYGFDYLRDNMVTQKSEKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGGGDEGTKLYELANAFIKNIRDEDFEMDRKDKTIALTASGLSKAEKFFGIEN
                     ITHIDNIEIFHHVNQSLRAHKLMNIDVEYVVRDNEILIVDEFTGRIMDGRRFTDGLHQ
                     AIEAKEGVKINDESKTMATVTFQNFFRLYSKLSGMTGTAKTEESEFEATYKINVVQIP
                     TNKPVIRADLHDKVFKTERAKYNAVVEEIIRINKTKQPVLVGTVSIEKSEKLSKLLKK
                     KGVKHEVLNAKQHDKEAEIISKAGKLGSVTIATNMAGRGTDISLGAGDKEEEEKVKEL
                     GGLYVIGTERHETRRIDNQLRGRSGRQGDPGTSRFFVSLEDEVIKLYGGKSIEKILKR
                     MNANEDDALESKALNRAIERAQKGIEGKNFEQRKNVLKYDDTINEQRKVIYDERNKVL
                     DGSDIEEEIQKMVMDIITEAAEKYLKKFRDYHGYFKYLYNTFMPADTLLIPDLDKKNV
                     EEIVNQTYQISKRVYDLKKMMIGIEELAQLEKRVLLQVVDTYWVDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYAIEGYEMFEALNRNIRITTVQYLYKFN"
     gene            21782..23653
                     /locus_tag="JA154_05630"
     CDS             21782..23653
                     /locus_tag="JA154_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat 2 family protein"
                     /protein_id="HAT7995340.1"
                     /translation="MNRKNLYVIMAAAMVSTSVAPSFAAEATQVKKQTITKKEATELV
                     SKVRELMAQRYTGGSQVGQPIYEIKVGETSSQLKIITNIDELEKLVNALGENKELLVT
                     IADKGHTTNSANEVVAEAIEKYENSADLSAEANSITEKAKTEATGIYGVATVEASYDS
                     TKDKLVITLKDKTDVVTSKTLQIGIGDEKVDLTANPVDIAGTNLDPSAEGFRVSKINK
                     LGVEGAKNIADIQLAEITIKNSDLNTVSPQDLYDGYRLTIRGNTIANSVGKSISDISS
                     KDSETGKYKFTVKYTDTSGKAIELTVESTNDKELKDVKDALNGNTKVNLIAGEDRYAT
                     AVAIAKETEYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKEI
                     IKKSPSAKIYIVGGESAVSNTARKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKEA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLTADAIKLMDGKEIGIVGGSNNVPSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKNDITNSQKNTLDKKLNLGAEVTQIGNGVELTVVQKIGKILGW"
     gene            23762..24469
                     /locus_tag="JA154_05635"
     CDS             23762..24469
                     /locus_tag="JA154_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HAT7995341.1"
                     /translation="MIEATLFFSPHQDDETLSMGSAIIEHVERSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESDFSKNRDEEFKNSCKAMGVKENNIHIENNRAH
                     DGELSKEKARDIMLKYLEKYPDAKVKTVTPYKALGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYNDFKKVNPNVRVWKVLSSQEEKLLNAMDAYKKWNPENGNYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            24502..26634
                     /locus_tag="JA154_05640"
     CDS             24502..26634
                     /locus_tag="JA154_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HAT7995342.1"
                     /translation="MKISKRIMSLLTATFFIFALSINIADASSKESLVGSGRWETAIK
                     ISQNGWKDATNAILVNDNSIADALSATPFAKLKDAPILLTQSDKLDSRTKAELKRLGV
                     KNVYLIGGMNTLSTNIEKQLKSENMTFERISGNSRYETSLKLAERLDKEKSISDIVVV
                     NGEKGLADAVSVGSAAAQENMPIILSDPNNGIKVANSFISSKNIKKSYIIGGTYSVSS
                     SIEQSLPNVKRLSGNNRNETNAKVLEEFYKNTDLKNAYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVVLVGNSLDSSQYDVLNTKSFDNIVQVGGNGNEDAVKDILDLQEETIYRV
                     KTADELDVALRKADANDIITFKPDDNKKITSSFSIETKKAITVELEGLYTQTITVNMP
                     NGELTNKGTLDGTVKIMDIKNGTFINEGDINDIDIYDPNGCKIENKSGGDIWVLTISS
                     SAKDVNIENKGDISKVTNNSTSVIIRNSGNIDTLSGINAPAISGNKPRVDDTDKDEKR
                     ASGIYPTSEPLSPARAGYISVKIFTKPEDTNYKIYYRVVSSRPSALYIGDRIDTSDWT
                     AVPSSVLPFTIKAENGYYIEAVEINSDTKKVSRWGKSDYIDDGFYLKSETAKGLSADI
                     DFDGDYVKLTATGADSGCTVYYRIVSKKPVSMNVGDKVTISDWDEITTNRVRIEANDV
                     KDKYIELVELDDSTNKVTRWGASDKVVE"
     gene            complement(26687..>26809)
                     /locus_tag="JA154_05645"
     CDS             complement(26687..>26809)
                     /locus_tag="JA154_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAT7995343.1"
                     /translation="IIMYIMIDLFFVNDITVKVFTNIIVIVLNYLFSKMIIFRK"
BASE COUNT        11184 a         2928 c         5052 g         7645 t
ORIGIN      
        1 aatttagtga tatatattta agtaataaga gtaaagcaaa aattagtgca tagtagtata
       61 taaaaatgga gtttgaatac ttttgttagt atatgattgt ttagtagaaa atcactataa
      121 aataggattt ctatctttga gcataattat agagagccta agtaggggga agggtttcaa
      181 gaagatatta ttagtgtttg tgttagtaaa ttatgtaaac acataaaata tattgaaaaa
      241 taatattata tataatatta tttatgtgtg ggaaatacga atataaaaaa taaaaaataa
      301 tattcggagg acataaatat gttaagcaac aaaaagagaa gtatggcaat agtaatggca
      361 ggtgctacag ttatgagtgc agcagcacca atattcgcag acaataccgt gacagaaaat
      421 gttgataaaa attacacagt aagtgcgaaa gattctgcta aactaatcga agaggttaga
      481 aaagcattag aagttaagtt tgaagataca aaagcaggag caaatgtaaa tgatagagtt
      541 tacgatataa aagtagacaa tgtaaactta actaatgcaa ctcaattaca aaataaaata
      601 aactctttaa cagaagggca aagtttaaaa gttactattc aagataaagg gcatcaagtg
      661 ttaggtggaa aagtagttga ctataaaatt gaaaactaca aaactgctca agaaatagta
      721 gatgcagtta atgcttacaa tgcaacttta gcagaagatt ctgataacaa gttaacagca
      781 actataaaat ctacaaatac agttgaagta aaaagagcaa aagattcagc aaatgttatt
      841 acattaaatg taggagacca acatttagat ttctcaaaag tgataacatc agaagaaggt
      901 acttttgaag gttatgaaaa gagttatagt gacatagatt caaaagaatt acatacgata
      961 acagttaaaa acgcagattt acaagacata tcagcagaag aattatttga tggaattaga
     1021 ttaactacat taggaagaga aatagttaat aaagttaaaa atggatatgc tttaacattt
     1081 gaaaatgaag caatccttac tcaagaacaa gaagatagtg atgataaaga taaaccagaa
     1141 aaatcaagct ttgatatagt tttaagcaag gcaaatgaaa agccagaaac aataagtgtt
     1201 tcaagtaaaa accataagtt agttagagac ttacacaaag tattaactga tgtaaaagat
     1261 ggaaaagaat taaaagtaga agttttatct ggtgattcaa gatttacaac agcagtagaa
     1321 gttagtaaag agagatttaa agatggagaa gcagaagcta taattttagt tggagaagat
     1381 gctatagttg atggattagc atcagcacca cttgcctctc aaaaaaatgc accaatatta
     1441 ttatctaaaa aagattcact accatcagaa atagaagctg aaatattaag agtacttgga
     1501 agtaatctat cttctaagaa aatatatata gtaggtggag aatctaaagt atcaaaagaa
     1561 actgaagaaa aactttctaa attaggtgta agtaaagttg agagagtttc tggagaagat
     1621 agatttgaaa cttctttaga aatagcaaaa caattaaaag atacatttaa gactgctttt
     1681 gtagtaggtg gaaatggaga agctgatgct atgagtatat cagctagagc tgctcaattt
     1741 ggtgctccaa taatagttac aggtaacgaa ttagatgcaa atgctgaaaa attattaaaa
     1801 ggaaaagaat tagaaatagt gggtggagaa aattctgtat caaaagaagt tgaagacaaa
     1861 ttagttgata tagatttaaa taataaagtt gaaagattag ctggagaaaa tagaaaagat
     1921 actaatgcta aagtaatcaa taaatactat gcaggtgcaa ctaaagcata tgtagcaaaa
     1981 gatggttatg taggtggaaa tggacaatta gttgatgcac ttacagcagc accacttgca
     2041 gctagttcaa aggctccaat agtattaact acagaagaac tttctaaatc acaagaagaa
     2101 gtagttgagt taagacttaa gaatgctact aagttagtac aaataggtga aggaatagct
     2161 aaaaacgcta ttgaaaaaat agcagaaaaa ataaacttat ttactaagaa ctaataagat
     2221 ataggaatta atattaaaag tttatttaga taatatatga ttatacaatg cgttaatatg
     2281 taagacatag tgtagatatt ataaaatata caaataacaa gataaaataa taaaaataag
     2341 gtgtctcatt tagaattaga ttgagatacc ttattttatt ttttgattat aatgttgaga
     2401 aattcatatt gcaaaaatta ttataaaaag atacttctta taatgattaa acatattact
     2461 aacataaatt attttgaact tataacgtaa ttaaataaaa taaattataa tgttttgaat
     2521 ttatagagaa taaaaacttt aggtatattg tttgagttgt atggataact atggtatcat
     2581 atataaaata tggtaacaaa attgtaactt ttgattaaaa ataagtatga gtactaataa
     2641 aaaatatgaa aagtacattg ggggtagaaa ataatatgaa gaatagtaaa aagatattag
     2701 ctataggact tacactattt ttagtaatgg taaatactcc tatggtaaat gcgttgacat
     2761 cagttgagca aataaaaggg aatgacagat atgagacagc agcaaaaata gcagataagc
     2821 aaaactacaa tacagcaata ctaatcaact cagataatag cttagcagat ggtctaagtg
     2881 caagtggttt agctggagcc ttaaatgcgc ctattttgat gacaaaacaa aaccagattc
     2941 caaacacaac tatggaaaga ttaaacaaag caaagactgt gtatataata ggttcagaat
     3001 caacaataag taaaaatgtt gagaatcagt tactatccaa aaagaaggtt gtacaaagaa
     3061 tatttggtga aaatagattt gatacaagta taaaaatagc tgaaaaaatc aaagaaatta
     3121 agccaataga taaagtaatt atagccaatg gatttacagg agaagcagat gcgataagtg
     3181 catcaccagt agctgctaga gatggagtac ctataatact tacagatgga aatagtgtgg
     3241 gatttgacac gacaggctta aagagttatg cgcttggttc tagtgaaata ataagcgacg
     3301 aattagttaa gagcacaaat tctattagat tgggtggcac agatagattt gaaactaata
     3361 agatagttat acaagagttt tataaaaata gtaaagaatt ttatctaagt aaaggcttac
     3421 aattaacaga cgctttggct gcctctacaa tagcaaaaaa tgctcctgta gttttagttg
     3481 aaaatggcag taacaagagt attttaagtg gtgcagacaa attgactgta ctaggaggca
     3541 taaatcagaa tgttatcaag caatgtataa atcaagcttc accaaatcaa caaggtttat
     3601 attacaatcc aaatgataga gcatttaaag agagaataaa gggtaaagta tatgctctta
     3661 caaagcagta tagaaaagag aatggagtaa gagcgttatc tgtagcaagc agattagagg
     3721 gtctagcaaa tgattggtct aatttaatgg caaataagaa gactttatct catacgataa
     3781 atggaaaaaa ttcatattct acttttttga aatacttaga ttggagtgag attaaacctg
     3841 gatatatagc agttcaaggt gaaaatataa ttaaatataa aattccagat aaacctgtat
     3901 atacaaatag agatgcagat gatataggaa actttatttt taatgagtgg aagacaaatc
     3961 cagaagaagg aactaatatg ttgcataaag ggtatgaaat aatgggattt ggtatagcta
     4021 taacaggaga taagaatcta tatgctacac atgaatttta tggaagatat aaagaataag
     4081 aaaatataca caaaattatg cttgtaagga agctatataa ataattaaat tataatggaa
     4141 aatagcattt atattaataa attaatataa atgttatttt tttataattt aatctaaaaa
     4201 aattatatta aaaagtttaa atatagtata aaatgggtaa gaaagtagtg aaaattaaat
     4261 ggcaaaatta gggaaatcta atgacgcaaa actataggga ttaaagcttt taaagccata
     4321 tcagccagtt gctaaaaaga gtaaagtctt tttattttat aattaagatt ctctttgagt
     4381 gtcttttttt attgtctgaa acttatagta tttaaattca tgaaaaatta gatatttatt
     4441 aagggaggat tgttatttca tgaaaaaggc aatatcttgt gtactagcag tatctatgtg
     4501 tagtgcacca ctaaacgttt ttgcagaacc tatattagag gggaagctaa gggcagttga
     4561 aaaatcagtt acagaaaagt taagggggag tcttgaagta gatttaaatt tttcactgcc
     4621 aataaaaaat agcgagtcag aaagtatgac taatatgcag ttacgattaa aagatgatag
     4681 taacaatagt ggaataataa agcttggaga aaaagattca ggaacagtaa atgttgggac
     4741 agataatata gaatatacta taaaaaaatt atctgcaagt agaagtgaaa taaaaaataa
     4801 agatgagaat atatcatatt ataatattgt atttaataat ttacctgttg gaaaatacaa
     4861 tgtagaagtc tctggagatg gatttaagaa caaagaaatt aaagacattg atatatcaag
     4921 ttattcacaa agagtcttat taagcaatag agcaagtgta gataataaaa ataaaccttt
     4981 aaattatgaa ttgttcttaa tgggtgatgt aaatggagac ggtaaagtag ataagagtga
     5041 ttataataag gttttagaga atatagattc aaataaaaga gaatttgatt taaatagaga
     5101 tggaaaggta gatattgttg acttagatta tgtacagaaa aatttaggac aaagtgaaga
     5161 tgcaaaaagc ctagaaagta tagtatctac caatcctata gtagatacaa gtaaggttga
     5221 attaaaagga aatagtgatg tagatattaa tggtaatgta gaaaatttat ttagtggaga
     5281 aaataatgga gttacaatat cttcaaaaaa tgatgaaata tctgaggaaa aaccagcagt
     5341 aatggatata gctttatcag aagcacttaa tatggaagtc atcaatataa aagctccaga
     5401 aggtactgct ccagcaaaag gaatagttga ggttactgac aaagatggta atgttcaaca
     5461 gattccttat gaaaacaaag aatatatgaa aaacttaaaa gatgaggagc aacaagagga
     5521 agtaaataac gaacaagaag aagtaaatca agaagaagta aataataaag atgaaaatat
     5581 aaaccagact gataataact caaataataa agatgaaaat aatgataaac aacaggaaaa
     5641 tagcgataaa caagaagcaa actctaatac caatcaagat aaaaataatg ataaagaaaa
     5701 ttcatcaatg gataaaccta attcagatga agatactcca aaagaagagg gtaatatcag
     5761 taaagatgat aactctacaa catctacata tagtaggtca gcattagaaa catatgcaga
     5821 tgttcaaaat agtgatatag taataaatct tggtaaacaa gtagcagtaa aaaagataac
     5881 tataaaaata acagctacaa cagcaaacga tagaaatctt gcagaaatat caaaagttga
     5941 atttttaaac aatgtatata aagaaatacc aaagccagag atgaacatac ctaaaataaa
     6001 taaaatatcg actagcactg ctgttggaaa tgaaaaagta tctttttatt ggaataatga
     6061 agtaaatgta tcagcatacg aacttatact agacaaaatt gatagtaagg gaaaagtatt
     6121 atctactaaa aaattacaaa ctagtaaaaa tacaatagaa gttagagata tagaagcata
     6181 tggactatat agagttagca tacaatcttt aaatggagat tggtctagtg gttataaaga
     6241 tgcaacacca gaaaacttag acggagtacc agaaaatgta gattcaaact ataaacctat
     6301 acaatttagc ccagatagta tattagaatt tcaagttgtt ccagacacaa aaccagaacc
     6361 accagaaggt ataaatgtta aaggtaaatt taaatctctt gatgtaagct ggaaaaatca
     6421 taaacaagct aaagattttg acttgtacta taaagaaaaa ggctctaatg ggtcttggat
     6481 aaaatacaat gataagctta ttcgtgggac aagttcaaag atagaaggtc ttaaggataa
     6541 tacaacatat gaagtcagaa tgacagctac taaccacttg ggaactagtg gaatgtctaa
     6601 aacatatata ggtacaactg aaagcatgga tgcaccaaca acacctaact atagattaat
     6661 taatactcct aaagaaaatg gtggatttgg aataccaaca aatcacattg tagatgttgt
     6721 ttatccagat ggatatgaca aaagtgaata tagtgataaa aatccatttg ataaattcaa
     6781 tgtagtagat ggggacttta caacacattg gacattccca acatataatg cagctgaggg
     6841 aacgaataga ggaccaataa taacttttga taaatcttat actatggata cttttatggc
     6901 tacacctcgt tttgatgttt ctgataagac aataaatgac tttgaggtac gatattggga
     6961 tgaaaatggt caattacaca atctggggga tgttcctttt gaaacaaaga cagaccctgt
     7021 aaataataat aagtatgtaa tggttagatt aaaagaacca ataactgcta ccaagataca
     7081 agtaagtgtt tgttcatcct attcatataa gtctaatata tcagaactta agttttatga
     7141 gtatgacagt atagaagatg atgtaagaaa tctatttatg gatggcctac aagttgaatt
     7201 aaaagaagat gtaactcaag aaaaaataac tgaattaaaa gatagattaa atactccaga
     7261 taaagctagt ggagaatatc atccatataa gacagttatt gagagagaat taaaattagc
     7321 tcaagactta tataatgata gagccacact tactgatgta actactgtaa atcaagaaat
     7381 aaataaatta ggaaaatata aagataaaaa cggacagtat actgtaaatt ccaataattt
     7441 aggtatgcaa aatgactggc aggcattagg agtagctgct agagcaggag atgaaatcac
     7501 agtatatgtt ggaagtaaaa gtggaaaaac tcctaagttg atatatacac agtactatgg
     7561 agaatctggt gcatataaga gtggagaaat aaatcttaac actggaaaaa atgtaattca
     7621 attaagcaaa cttcatagtt tagatataga atgtggagga agtttataca taagatacgc
     7681 tgaagatact ccaagtggag gagatataaa agtacgtgta gcaggagcta ctaagatacc
     7741 acatcttaat ctaaatggaa caataaatga taagagtcct gcgggagttt ctgaatctaa
     7801 gaaaaagata aaagcatata tagaagaatt agctaaatat aaagatgctg taaaaggaga
     7861 accatattat ccaggaacaa atgttggtaa ttcatatgga tattcagaaa aaactggtgt
     7921 gttaaacact acagatattg aaagtgataa agtaacatta aatgttcctg caacagcagt
     7981 atatcaagag atatcaaaag gaaattctga tttaaataca caagtagata gattatataa
     8041 tagtatgctt gcatgggaac aaataatgga tttagtttac tcagaaagag gagtatttaa
     8101 aacacaagat ttagatggta atggaactgt agatgataat gaatttaact taactaaaaa
     8161 tgatatggct ccaaaatctc gtatgaacat aaaataccaa agaatgttta ttggagcatt
     8221 tatgtatgct tctggattac atgtaggagt agaatatggt tcagttccag ggcttatgaa
     8281 tggagtacct ttccaaatag atgatagtgg aaaagctaca ggaggaaact tatttggatg
     8341 gggaataggt catgagatag gacatgttac agatattgga aagatgacat atagcgaaac
     8401 tagtaacaat gtacttgctt tacttgctca gacctttgat gataagacac attcaagact
     8461 tgaaggttca acaatggata agatatatga gcatgtaact tcaaatagct taggtatacc
     8521 aagcaatgta tttgaaaggc ttggaatgtt atggcaatta catctagctt atgatgatga
     8581 ttttacgggt tcaatgttaa agaataactc agatgctgat ttaagtaatg atacattcta
     8641 tgcaaagatt tcaagaaaat acagagcact ttcaagtagt gacccaatta atagtttacc
     8701 aaaagaccaa atgctagtag caatggcatc aagtgttgtt gaaaaagatt taagagatta
     8761 ctttaaggca tggggagtag aaattacacc agaattaaac agtataatgg atagcaagaa
     8821 ctacgaaaaa gaatcaagaa agatacaata tgtaaatgat gaagctagaa gaaagatttt
     8881 aaatgaaaat atagctagta tggcaaaaga tactaaagta agtgctagtt tttctgatgg
     8941 aatacaaagt ggttctctag ttaaatctaa taaaatatct atagacctag atgtagataa
     9001 ggataaagat aagatactag gatacgaaat aataagaagt gatggaaatt atttagatgg
     9061 tgaaaatgga acacaggtaa aatacagaac agttggattt gtggatgcaa aaggaaatag
     9121 caagactaaa tttactgata atatatctcc tcttaataac agagcattta catataaggt
     9181 agttgcatat gattatcacc taaatccaac agaagaattt gaagttggta ctgtaaaatt
     9241 atcagatgaa gggaaaataa ataaatcagc atggtcattc ataacaaata ctgtttctga
     9301 cggtgatgtt agaactgaaa atgactcaca tggaccatta caaaatcctg aaattgataa
     9361 tataaaagat aatgatgcaa gtactacata taaaggaaag ataataagta aagcagagtg
     9421 gaacaaagac cctcaaaaag accctgatat aaatgtagaa gaaaatccat atataatagt
     9481 agacatgaaa gaaacattac ctatagtagg actaaaatat acaaaaccag agacagaagc
     9541 tagaaagttc tcattaaaag gattatttaa ctttagaaag aatactagca caacatacaa
     9601 tcctttaact aattacaaga tacaagtaag taatgataaa aagaactggg aagatgtaag
     9661 tcaaggaact tttgaatatg gacaagattt agttggtgga ggaaaagacc aagataatga
     9721 agctagagtt acatttaata aagatggtaa actttggaca taccaagcta gatatgtaaa
     9781 actaatctca aataataagt ctaatataga agttgcagaa ttagatataa taggacctcc
     9841 aggtgataat atagaaattg gcataataaa taatgcagac gaaactcgta ctaatggaat
     9901 tggaaaatta gcagaagatt atgtatatca aagtgatgac tctagcacaa gtgaagatga
     9961 aacaggtaag attccagcgg gttcaatagt tattacaggt gaatacagtg gaaatccagc
    10021 atttaattta ccactactta tagataaaaa caataagact atatctggag aagcactttt
    10081 atttgcagaa gttccagaaa aaggtgaact aggtgaaatc agtaaaggta catgggttta
    10141 ctatatgcca aaagaaaact ttgattcttt aagtgataag gttaaggctg aactatatag
    10201 atacaatgac cttcaaggag atactccagt aggtcaaaga tttgtaagtg atacaatgta
    10261 tgtacctgta ggagctaaga attatgatga gttaaagaca ataagtctta ctgatagtaa
    10321 tagtaaagct agaaaagcta caagaaattc tattgatatc agtaataaga aggttataac
    10381 tgtaagcaat aaagtaaagg gtaatattgt tagagataaa tagtaggtgg aactctaaaa
    10441 acgtaaaaat actaacattg tatatctaat aagattaggt actagaatag ttttaaaata
    10501 gtagatatta aaagtaaagt atataaatgt aaccataaat ttagggtaat ttataaatta
    10561 aatataaaag ctaggtggat aagtaaattt atccacttag ttaatgatag ataaggacgg
    10621 gtgttaatga gtaaaagaaa atcttttata agaactatag cagtatcaac tatggcagta
    10681 gctgtaactg gtagtgctac atgtgcatat gcagctccag ttttacaagg aacaaaaacg
    10741 tatgagaaag taaatactat agatatatct gtggactcag tagaaaacat agtatattca
    10801 tttcaagcca gtataaaagt acaaggtgag gttgaagtag tagataatga gcagaaagaa
    10861 aagattactt ggtctgataa tataaaatct cagataaaaa gtggaaatgc aaatgctact
    10921 tgtagagtag aatacaacaa atcaagtaat acaactacat tagatatata tgttacatca
    10981 aatgaagatt tactagacgg aaatagattg aatataggaa gaatatctgt aaaaaaatct
    11041 ggcagtaact caaatgctga ctataaagtt ttaggaaaag gaacaaatga taaaccagct
    11101 ttaaaaattg taacatataa caacaaaaca gtggattatg aaaatataag ctctgatgaa
    11161 ggattaatat ttacccttat taatgagagt gaagtaaaac caattggtgg aactggttct
    11221 agtaaaaatg accctgaaaa gtataaggta gagaaaagtg aagcacttga atatttatta
    11281 aacaatatta ggataaacta ttctattgtt agtaaagaaa cacaagaaag tggttcaaat
    11341 gtaatattaa aacttggatt agcacaaaaa acaactaagg gaagaaaagc tacaataaac
    11401 aaatatgttg aagttacact tccaaaaagt ttagaataca tagttgaaaa tgaattatca
    11461 aaaccggatg aattaccacc agataatggc aatggtggaa ataatggtgg aggaagtaat
    11521 tcaggcggct catctagtgg tggaagctct ggaggaggaa gttcaagtga ttctacatct
    11581 aatgtcacag tcaaaaaatt aaaaggtgct gatagatttg agacagctat caaaatatct
    11641 caaagtggtt ggacaaaatc agatacagtt gtaatagtaa atggagaaga taaaagtatg
    11701 gtggatgggc ttacagctac accacttgct agtgtaaaaa actcaccaat tcttttatca
    11761 tcaaatgaaa agcttcctca aaaaactgta gaagaattga aaaggctaaa tccatcaaaa
    11821 gtaattgtga taggtggaaa caactcaatg ccaaattcag ttgttgaagc tatcaaagct
    11881 gtaaattcaa aaatatcagt acaaagaata ggtggagata caagatatca aacttctatt
    11941 aatatagcaa aagaaataga taagacgaat aatgtaagca agttgtatat aggagcagga
    12001 aatggagaag ctgattcatt atcaatagca tcactggctg gtaaagaaaa aactccaata
    12061 gtacttacac aaaaagatgg tgtagataat gaagctgaac aatttattaa atcaaataag
    12121 gtgtcaaata tatattttat tggtggagta gaaaaaatat ctaacaaggc tattgaacaa
    12181 gttggaaaaa tagcaaacaa agatatatca aacaataggg tggcaggtca aactagacaa
    12241 gagacgaatg ccaaagtaat agataaattc tactctcaat ctaagctaga tggagttgta
    12301 gttgcaaatc aagataaact tatagatgct ttagcagttg gaccattagc agctaaaaac
    12361 aattcaccag ttatacttgc tacaaatacg ttagataaat cacaagaatc gtcattaaaa
    12421 ggtaaaaatt catcaaagtt gtttgaagta ggtggaggaa tagcctcatc tgtaatagac
    12481 aagattaaga gtttaattga aaaataaata ttaagtaaat agaaaataag aatgtattta
    12541 gatataaagt tatatattta aatgcattct tattttattt tcattatctt aacaatctaa
    12601 taatattgag gttaaaatgt ataataattt gagctatatt aagtttaatt ttaaataaag
    12661 cagatatctt ttatatatat aaatataaat tcaattttga atatgtttaa aaagaagtta
    12721 aaaataattt tttaaattta ataaaataaa acactaatat atagacgaga tatattcata
    12781 tcatatatac tctataatat atggaaaata aagagaaaat aaatggaaaa taatcacaat
    12841 ataaatataa aatataaaaa tcatcagggg gatatgaaaa tgaacaaaaa aatattatca
    12901 ttaggtctag cagtatcatt aatcttagta aacttcaaaa gtgtaaatgc atcatcagta
    12961 gtagaaaaaa tatacggtaa agatagatat gaaacagcag caaagatagc tgataaacag
    13021 acttatgaaa cagtaatttt agtaaataca gaaaaatcac ttgcagatgg attaagtgca
    13081 agtggattat cgggagctac aaaagctcca atactattta cacaacaaaa taagatacca
    13141 gcagacacaa atagatgtct aaaaaatatc aaaaaagcat atataatagg tacagaagat
    13201 actataagta aatcagtaga aaaagaacta gattctaaaa atatagaagt caaaagaatt
    13261 ggtggagaag atagactaaa aacaagctat ttaatagcta aagaaatagc aactataaaa
    13321 aaagtggata aagtactatt aactaatgca tatagtggag aagcagatgc aatgagtgta
    13381 tcatcagtag ctactagaga tggagctcca atcatactta cagatggaaa gagtgtgcct
    13441 tttgatgtaa aaaatattca atcatattgt ataggttcag aggagataat gagtaatcct
    13501 ctagttaaaa atacaaactc agtaagaata gaaggaactg accgatttga aactaacaaa
    13561 aacgtaatag actacttttt taatagtgca gatggattct atgtatcaga tggataccaa
    13621 ttagtagatg caatagcagc tgcaccactt actaaaaact ctccaatggt actagtaaat
    13681 gatggaagcg ataaaactgt attagaagga gctaagaata taacctctgt aggtgaaata
    13741 aatgagaagg taatacaaca gtgtataaat gcttcaaatt caaatgggca acccccaaca
    13801 attacagttg gaagtacaga ggtatataag ggtgaaaagt ttgacactgg caagttaaat
    13861 atagtagcta aagataacac agggaaggta ttaccaatag aagttgatgg gtttatagat
    13921 acaaatagag taggtacata catattgaca ttaaaagcta cagacgaatg gggaaaaagc
    13981 acaggaaaaa gagtagaaat aaaagtgttg gatgacaaat ctcatgatta caacagccca
    14041 gaatttaaaa aaatggtatc tactgaaatg tataacttaa tcaattccta tagaaaagaa
    14101 aaaggcaaag agcctttgct agtgtctagt aggctagaag gtatggcaaa tgcatggtct
    14161 aagtatatga tggataaaaa agtatttgca cattatatag atggaaaaaa tgctccacaa
    14221 gtattttctg aatttggaat gagaagtgag gaaaatatag catatattta tatagattct
    14281 aagaacgttc aaacgacaca agatgcaaaa gacttggcaa aggctatatt tgaggtgtgg
    14341 aagaagtctc cagagtacaa tgcaaacatg cttagtaatg agttttatag tactggattt
    14401 gggctttata tattatctga tggtcaagtg catgctactc aggagttttt aaatggaaat
    14461 gaaggtagct tgtaaaaaat tccataaatc ttgttttttt gttaatttac tgatattata
    14521 aaatgaaaat cacatctaaa gtactttttg taatctgttt tgttattgct ttacaggcta
    14581 tggtatttta ggtgtttttt gttttagtag tttatagatt ttgagggaat cactactgag
    14641 gatgtaattt atgattagct gaattatata aaaaaattag taggaggagt ttgatgaagg
    14701 taaataagag agtattatca ataggattga caatatcatt aataatggca ggagcaccaa
    14761 atatcaatgc actttcgagc atagaaaaga tacaggggaa agatagatat gagacttctg
    14821 cattgatagc agataaacaa atatatgata caatcatatt agtaaatact gataattcta
    14881 tagtagatgg attaagtgca agtggattat ctggagttgc aaaagctcct ataatgttgg
    14941 tgcaaagaga taagatacca acagatgttg aaaaaagact aaaggatgtt aagaatgctt
    15001 atgttatagg tacagaggat acaataggta aatctgtaga aaatcaactt aagaataaag
    15061 ggattgaagt taaaagaatt ggtggggaag atagaataaa gactagctat cttatagcta
    15121 aggagatttc agctattaaa ccagtaaatg atggtgataa ggtttttctt gtaaatggat
    15181 acacaggtga ggctgatgca atgagtgtat cttctgtagc tgctagagat ggagttcctg
    15241 tgatacttac tgatggaaag agtataccat ttaaagttga tggcgttcag tgctattctc
    15301 ttgggtcaga ggagataatg agtaatgaac ttgtgagtaa gactaattca gttaggattg
    15361 ctggtaagga taggtttgaa actaataaga aggttataca acgcttttat aaaggaacaa
    15421 agaagtttta tgtttctcaa gggtataagt tagttgatgc tgtggctggt tctccgttgg
    15481 ctaaggacaa acctatagta cttgttaatg atggaagtga taagagtgtg cttagaggtg
    15541 ctgatgaagt tacttcttta ggtggaatgg ataagaaggt tgttgaccaa tgtattagtt
    15601 ctgcatctga taaaaataca atgcctacaa ttactgctaa tgatgtagag atttctgtag
    15661 gggataagtt tgataatagt atgttgaata tagttgctac tgattattat ggaaatgatt
    15721 taaaggcaaa tattaaagga aatgttgata ttaacaaggc tggaacatat gttttaaata
    15781 taagtgtagt ggataattta ggtcagaaga gtgagattag tgtaaatgtt aaggtagttg
    15841 taaatgcgag tacaaaagac tctaatagtt atgagtttaa ggctatggta tctaatgaga
    15901 tgtatgattt agttaattca tatagaaaag aaaaaggcaa gaagagttta agagagttag
    15961 actctttagc tggaatggca aatgcatggt ctaaatatat ggaagataag aaggtatttg
    16021 cccatgagat tgatggtaaa aatgcagctg aagtattttt tggctttgga gcaagaagtg
    16081 gtgaaaatat tgcatatcta ccaatgaatg ttaaatctgt ttatacttct aaagatgcaa
    16141 aagaaatggc tgagtctatt tttgatttat ggaagaagtc ttctaagtat aatgaaaata
    16201 tgttaaaaga agaattttat agctttggat ttggtatgca tgtttctagt aaaggagaag
    16261 tgaatgcgac aatggaattt ttaaatagtt agattttata ttaatttaca ttggaaaaga
    16321 tatagtaaat ttgtaaaaat aaatatataa aattaaaata acttataaaa ttataaaaaa
    16381 taatttaaaa ataataaaaa tgatgaaaaa acaattaatt tgttggaaaa aacttcaatt
    16441 aattttgcat aaattattga atttatattt tatatagtct atcataagag tgtgagaaat
    16501 atttggatta tatgaacatg gaaatttaat gtacataatt aaagagatgt aattaatata
    16561 atgttgggag gaatttaaga aatgaataag aaaaatttag caatggcaat ggcagctgtt
    16621 actgttgtag gttctgctgc tccgatattt gctgatacta ctgttaaaga agaaggatat
    16681 acggtagtac aagacaaata tgaaaaatta cttaaagaat taaaggcaaa gataaaagat
    16741 ggaactatta catcagttgg tgttgaattt gatggaaaac caattactac attagcacca
    16801 aaggcagatg gtagtgataa agatgcaata gcagaacagt tagaaactct tactaaaaac
    16861 caacttaaag gtttaggtga tggaaaatat gtagatttta aaattactta tggtgctaaa
    16921 gcagaagttc cagctgcttc attaagtgca gatgatatac aaaaatatgc agatcaaata
    16981 aatgcttctg agaaaatatt agttgaagta gcagcaggat ctgaagcagg tatagcaaaa
    17041 tttgattctg ttaacaataa agttatagca ggagatgctc ctcttaaagt taaagatgca
    17101 gtgaaagcga ctgttactac taatggtagt aataaaaaag ttcttacaat atcagctgca
    17161 gctggattat caggattttc atatggaaca cttaaagaca aaggtgcaag ttcatctgat
    17221 gttgatgcaa taactcttga cactacaaat gcaacaataa cagaaggtga tactaaagta
    17281 ttagattttg ataattcatt taagtttaac gaaagtacta aaaaagttgg tagtttagtt
    17341 acaccaaata caacaaacac tccagcagat cctggtacta aaactactgt tagagtgata
    17401 aaagctgttg aaaaaacaat agatgtaagt tcaaattcaa ctacaaaagc taaagattta
    17461 gctaaacaat atgtattcac agatgttagt gatactgatc cagaaagttt aagttatatg
    17521 cttaaaaaca taaatgatgg aaaagttgct gttaaaaatt ctgatggaga ttatgaagta
    17581 actatattcc cagaaggaaa aagattaaat actttatctg cttcttcagc taaaactatt
    17641 ttaggagata aagatactcc agcaaaaata gttttaaagg cttcaacaac aaagaaatta
    17701 gcagactata tagatgattt aataacttat aacaatagtt attcaaatgt acaaactgta
    17761 gcaggtagtg atagaataga gactgctata gagctaagcc gtaagtacta taactctact
    17821 gacaaaaatg ctttatatgg agatcctgta aataacgttg tattagtagg ttctcaagct
    17881 atagttgatg gattagtagc atctccttta gcagcagaaa aagatgctcc tttattgtta
    17941 agttctaaag ataaattaga ttcatcaaca agagctgaga taaaaagagt tatggactta
    18001 aattcttcaa caggaataaa aaataataaa gaagttttca tagcaggtgg agtaaattct
    18061 atatctaaag acgttgaaaa tgaactaaaa gatatgggac ttaaagtaac tagattatca
    18121 ggtgatgata gatatgaaac ttcattagcg atagctgatg aaatagatat aaatgataaa
    18181 gcttatgtgg taggtggaac aggattagca gatgctatga gtattgctcc agttgcatct
    18241 caaataaaag atggtgaagc tactccaata gtagttgtag atggaaaatc tgacaagtta
    18301 tctaaagaag ctgaagattt cttagatgat gcacaagtag atataatcgg tggagaaaat
    18361 agtgtttctg ctaaaatgga agattatata gatgatgcta ctggaaaatc tccagaaaga
    18421 attagtggtg ctgatagaca agcaactaat gctgaagtta taaaagaata ctttgataaa
    18481 gatggagttt caaattactt cttagcaaaa gatggttcaa ctaaagagga tcagttagtt
    18541 gatgcattag cagcagcagc agtagctgga aactacggtt ctaagcataa tgaagatgga
    18601 gatataacta cagatgcttc tcctgctcca attattttag caactgataa tttatcagct
    18661 gaacaacatg tagctgtaag taaaactgct actactaatg gtgctaagaa cttagtacaa
    18721 gtaggtcaag gaatagctga ttcagttgtt agcaaattaa aagatttatt agatatgtaa
    18781 tataaatttt atatattgaa taaaaaaggt ttctcttttg agaaaccttt tttattttaa
    18841 acataaattt ttataaataa aaagaggcta attagcctct ttttaatgtg agaaacactt
    18901 aaaaaataag atgtaattat ttatttaagt atcattatta tactatacct taaaataaat
    18961 gtctactatt ctataatttt aaggtataat agtaagtgga aaataaataa ggaggtaaat
    19021 taaatatgtc agttttagat gtaatattag acaaaacaga tgaattagaa attaaaaaat
    19081 taaataatat agtagataaa atagatgctt tagaagataa aatacaaata ctagatgatg
    19141 aagcattaaa aaatatgacg aatgtattta aggataagtt aagtaaagga gaaaccttag
    19201 atgacatatt gccagaagca tttgctgttg taagagaagt atcaaaaaga atattaggta
    19261 tgcgtcaata tagagttcaa ttaattggtg ggattgtatt acatcaaggg aaaatagcag
    19321 agatgagaac tggagaagga aagactctag taggagtagc tccagtttac ttgaatgcat
    19381 tgactggtaa gggggtacat gtcatcacgg ttaatgatta cttggctcaa cgtgataaag
    19441 aacttatgca accagtatat gaatttttag gtttaacagt aggagtaatt attaatgggc
    19501 aacagccagt agaacgaaaa ttacaatatc aatgtgatat tacttatggt acaaacaatg
    19561 agtatggatt cgattattta agagataata tggtaacgca aaaatcagag aaagttcaaa
    19621 gagaactaaa ctttgctata gttgatgagg ttgattcaat acttatagat gaagctagaa
    19681 caccacttat aattgctggt ggaggagatg aaggtactaa gctgtatgaa cttgcaaatg
    19741 cttttataaa gaatataaga gatgaagatt ttgaaatgga tagaaaagat aaaaccatag
    19801 cacttactgc aagtggtctt agtaaagctg agaaattttt tggaatagaa aatataactc
    19861 atatagataa tatagaaatt tttcaccatg taaatcaatc tctaagagca cataaattaa
    19921 tgaacataga tgttgaatat gttgttagag ataatgagat actaattgta gatgaattta
    19981 caggaagaat aatggatgga agaaggttta ctgatgggct tcatcaagca attgaagcta
    20041 aagagggagt aaaaataaat gatgaatcaa agaccatggc aacagtaaca tttcaaaatt
    20101 tctttagatt atactctaaa ttatcaggaa tgacaggtac agcaaagaca gaggaaagtg
    20161 agtttgaagc tacatataag attaatgttg ttcagatacc aactaataaa ccagttataa
    20221 gagctgattt acatgataaa gtattcaaaa cagaacgtgc aaagtacaat gctgttgtag
    20281 aagaaataat aaggataaat aaaactaagc aaccagtgct tgtaggaact gtttcaattg
    20341 aaaaatctga gaaattatct aagctactta aaaagaaagg tgtaaagcac gaggtcttaa
    20401 atgctaaaca acatgataag gaagctgaaa taatatctaa agctggtaaa ttaggttctg
    20461 ttacaattgc tactaatatg gcaggtagag gaactgatat atcacttgga gctggagata
    20521 aagaagaaga agaaaaggtg aaagagttag gtggcttgta tgttataggt actgaaagac
    20581 atgagacaag aagaattgat aatcagctta gagggcgttc gggaagacaa ggtgaccctg
    20641 gaacttctag attctttgta agtttagagg atgaagtaat aaaattgtat ggtggaaaaa
    20701 gtatagaaaa aatattaaag agaatgaatg ctaatgaaga tgacgcgtta gaaagtaagg
    20761 ctttgaatag agcaatagaa agagcacaga aaggcattga aggtaaaaat tttgaacaaa
    20821 gaaagaatgt tcttaagtac gatgatacta ttaatgagca aagaaaagta atatatgatg
    20881 aaagaaataa ggtattggat ggttctgata ttgaagaaga gatacaaaaa atggttatgg
    20941 atattattac ggaggctgct gaaaagtact taaaaaaatt tagagactat catggatatt
    21001 ttaagtattt atataatact tttatgccag ctgatacttt attaattcca gacttagata
    21061 aaaaaaatgt tgaagaaata gtaaatcaaa catatcagat ttcaaagcgt gtttatgatc
    21121 ttaagaagat gatgattgga attgaagaat tggctcaatt agagaaacga gtattacttc
    21181 aagttgtgga tacttattgg gttgaccata tagatgcaat ggatcaacta agacaatata
    21241 taggtcttaa atcttatgca caaaaagatc catttaaaga gtatgctata gaaggttatg
    21301 aaatgtttga agctttaaat aggaatataa gaataacaac agtgcaatat ctatataaat
    21361 ttaactaaaa aagtgaccca ttgggtcact ttttatatat taagattaaa gtgaaattta
    21421 ttttacagta cacactcata tagataaata tttataataa tgaaaaagag gctgaacgtc
    21481 agcctctttt cctgtgtgag aaatacggaa aaaagagatg cttgaaattt ctttcttcca
    21541 tttaatatta tactataaag agaataaaaa gtccatgagt tatctttatg attaaaaaaa
    21601 aaaattacaa ttttgttact ttttagtata caataataaa tttaaattga ctttgttaat
    21661 ttttattaat aaaatatcca aatattacaa tatctacagt ttattgaaat aatgtgtaaa
    21721 ttatattata attggtaaat aaggaaaaat ggtgaaaacc aaattttgta gggggaaata
    21781 tatgaataga aaaaatcttt atgtaattat ggcagctgca atggtaagta catcagtagc
    21841 tccaagtttt gctgcagaag ctacacaagt aaagaaacaa acaataacta aaaaggaagc
    21901 aacagaatta gtttctaaag ttagagaact aatggctcag agatatacag gaggttctca
    21961 agttggacaa ccaatatatg aaataaaagt tggtgaaact tcatcacaat taaaaataat
    22021 aactaatata gacgaattag agaaattagt aaatgcttta ggagaaaata aagagcttct
    22081 tgtgactata gcagataaag gacatacaac aaatagtgca aatgaagtgg ttgctgaagc
    22141 aattgaaaaa tatgaaaatt cagcagacct ttcagctgaa gctaattcaa taacagaaaa
    22201 agctaaaact gaagctactg ggatttatgg agtggcaact gtagaagctt catatgacag
    22261 tacaaaagat aagttagtaa taactttaaa agataagact gatgtagtaa cttctaaaac
    22321 gctacaaata ggtattggtg atgaaaaggt tgatttaaca gcaaatccag ttgatatagc
    22381 aggaaccaat ttagaccctt ctgcagaagg atttagagta agtaaaataa ataaattagg
    22441 tgtagaagga gctaaaaata ttgctgatat tcaattagct gaaataacta taaaaaatag
    22501 tgatttaaat acagtttcac ctcaagattt atatgatgga tatagattga ctattcgagg
    22561 taatacaata gcaaatagtg taggaaaatc aattagtgat atttcatcaa aagattcaga
    22621 gacaggaaag tataaattta ctgttaaata tactgataca tctggaaaag cgatagaact
    22681 tactgtagaa agtactaatg ataaagagtt aaaagatgtt aaagatgcct taaatggaaa
    22741 tacaaaagtc aatttaatag ctggggaaga caggtatgca actgctgtag ctatagcaaa
    22801 agaaacagaa tatactgaca atatagttat agttaattca aataaattag ttgatggatt
    22861 agcagcaaca ccactagctc aatctaaaaa agctcctata ttgttggcgt ctgataatga
    22921 aataccaaaa gtaactttag attatataaa ggaaataatt aaaaaaagtc catcagctaa
    22981 aatatatata gtaggtggag aatcagcagt atcaaataca gctagaaagc aattagaatc
    23041 agtaactaag aatgttgaaa gattggctgg agatgataga catacaactt cagtagcagt
    23101 agcaaaagct atgggttctt ttaaagaagc atttgtagta ggtgcaaaag gggaagctga
    23161 tgctatgagt atagctgcta aagctgctga acttaaggct cctataatag taaatggttg
    23221 gaatgatctt acagcagatg ctatcaagtt gatggatgga aaagagattg gtatagttgg
    23281 tggttctaat aatgtaccta gccaaattga aaatcaactt gctgatattg ataaagatag
    23341 aaaggttcaa agagttgaag gtgaaacaag acacgatact aatgctaaag ttatagaaac
    23401 atattatggc aaattagata aactatatat agcaaaagat ggatatggaa ataatggtat
    23461 gttagtagat gcattggcag caggtcctct agcagcagga aaaggaccaa ttcttttagc
    23521 taaaaatgat ataacaaact cacaaaagaa tactttagat aaaaaactaa atcttggtgc
    23581 agaagtaact caaataggta atggggttga attgactgta gttcaaaaaa taggcaaaat
    23641 actaggatgg taataatcaa taatatagaa aaggattcct tattatttat aggaatcttt
    23701 ttctatcgac ataatattca ttaaagtata acatgctctt gacagatagg aggatatgat
    23761 agtgatagaa gcaacattgt ttttttcccc acaccaagat gatgaaacat taagtatggg
    23821 tagtgcaata attgaacatg tagaaagaag cgatacacat gttatattat gtactgatgg
    23881 cagtaaatca ataataagaa aagtattaga tgatggaggg tcatgttctt atcatattaa
    23941 agatgtacac aagtactctt tatcagaaag tgatttttca aaaaatagag atgaagaatt
    24001 taaaaatagt tgtaaagcaa tgggagttaa agaaaataat atacatatag aaaataatag
    24061 agctcatgat ggagaattga gtaaggaaaa agcaagagat ataatgctaa aatatttaga
    24121 aaaatatcct gatgctaaag tgaagaccgt tacaccatat aaagctcttg gaatacatga
    24181 agaccataga gcattaggtg aagctgcatt agaattgtat agagaaggaa aaataaaaga
    24241 tttaagattt tatgtagagc catacgatta taatgatttt aaaaaagtta atccaaatgt
    24301 tagagtatgg aaggttttga gtagtcaaga agagaaatta ttaaatgcta tggatgcata
    24361 caagaaatgg aatcctgaaa atggtaacta cgcaattgga tatcattctg taaaatctca
    24421 ctttgatgaa ttaaaatcca ataaaatcca atatgtacat gctccataaa cattaatata
    24481 aaaactagga ggatatagtt aatgaaaata tctaagagaa taatgtcgtt gttgacagca
    24541 acttttttta tctttgcatt atctatcaat atagctgatg cttcatcaaa agagagtcta
    24601 gtgggctctg gtagatggga aactgcaata aaaattagtc agaatggatg gaaagatgct
    24661 actaatgcta tactagtaaa tgataattct atagctgatg cattatctgc aactccattt
    24721 gctaagttaa aagatgcacc tattttatta actcaaagtg ataaattgga tagtagaact
    24781 aaagcagaat taaaaagact aggtgtaaaa aatgtatatt taattggagg tatgaatact
    24841 ctaagcacca atattgaaaa acaattaaaa tcagaaaata tgacttttga gaggatttca
    24901 ggtaatagta gatatgaaac atctttaaaa ttagctgaaa gattagataa agagaagagt
    24961 atttctgata tagttgtagt taatggagag aaagggctag cagatgcagt aagtgtgggt
    25021 tctgctgcag cacaagaaaa tatgccaatt atactttcag atcctaacaa tggaataaaa
    25081 gttgcaaata gctttattag tagtaaaaat ataaagaaat cttatataat tggtggtaca
    25141 tattctgtat ctagttctat tgaacagagt ttacctaatg taaaaagatt atctggaaac
    25201 aatagaaatg aaactaatgc aaaggttcta gaagaatttt ataaaaatac agacttaaaa
    25261 aatgcttata taactaaaga tggtatgaaa aaacaggggg atttgataga ttctctagct
    25321 gtaggtgtat tggcagctaa aaatagctct ccagttgtat tagtaggaaa ttcacttgac
    25381 tctagccaat atgatgtgtt aaatactaaa agttttgata atattgtgca agttggtgga
    25441 aatggaaatg aagatgctgt aaaggatatt cttgatttac aagaagaaac tatatataga
    25501 gttaagacag cagacgaatt ggatgttgct ctaagaaaag cagatgctaa tgatattata
    25561 acatttaaac ctgatgataa taaaaaaatt actagttcat ttagtataga aacaaagaaa
    25621 gctataactg tagagcttga aggattgtat acacaaacaa taactgtaaa tatgccaaat
    25681 ggagaattaa ctaataaggg tacattagat ggaactgtga aaataatgga tataaaaaat
    25741 ggcacattta tcaatgaagg tgatataaat gatattgaca tttatgatcc aaatggatgt
    25801 aaaatagaga ataaaagtgg tggagatata tgggttttaa ctatatctag tagtgctaaa
    25861 gatgtaaata tagaaaataa aggtgatata agtaaggtta caaataattc tactagcgtt
    25921 ataataagaa attcaggaaa tattgatact ttaagtggaa ttaatgcacc agcaataagt
    25981 ggaaataaac ctagagttga tgataccgat aaggatgaaa aaagagcaag tggaatatat
    26041 cctacttcag aacctttatc acctgcaaga gctggttata taagtgtaaa aatattcact
    26101 aagcctgaag atacaaatta taaaatttat tatagagtag taagcagtag accatctgca
    26161 ttatatatcg gagatagaat agatacaagt gactggactg ctgtaccatc ttctgtttta
    26221 ccatttacta ttaaagcaga aaatggatat tatatagaag ctgttgaaat aaattctgat
    26281 actaagaaag tatctagatg ggggaaatct gactatatag atgatggatt ttatttaaaa
    26341 tcagaaactg caaaaggatt aagtgcagat atagattttg atggagatta tgttaaatta
    26401 acagcaacag gtgctgattc tggatgtaca gtttattata gaatagtatc aaaaaagcca
    26461 gtatcaatga atgttggaga taaagtaact atatctgatt gggatgaaat tactactaat
    26521 agagtgagga tagaagcaaa cgatgtaaag gataaatata tagaactagt tgaattggat
    26581 gattcaacta ataaagtaac tagatgggga gcaagtgata aggtagtaga ataggtatct
    26641 aatgtgaaac ataaaaacag actgattaat aatgggcagt ctgtttttat ttcctaaaaa
    26701 ttatcatttt actaaataaa taatttaaaa ctataacaat tatattagta aatactttta
    26761 cagtaatatc atttacaaaa aataaatcaa tcattatata cataataat
//
LOCUS       DACULQ010000032        26726 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.032, whole genome shotgun sequence.
ACCESSION   DACULQ010000032 DACULQ010000000
VERSION     DACULQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.032"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..704
                     /locus_tag="JA154_05650"
     CDS             <1..704
                     /locus_tag="JA154_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT7995344.1"
                     /translation="KYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKFFTVPNTIV
                     RLCKKMEYDGFSQLKNNLKEELKNEDKEAKNGFESDIKKTLELIDYEKINKVADIIQK
                     SRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQDMFFALSLS
                     GETKEVIEVAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNGYNISDKVP
                     IMMIMRAIIEQYWLR"
     gene            834..2759
                     /locus_tag="JA154_05655"
     CDS             834..2759
                     /locus_tag="JA154_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089600.1"
                     /note="involved in the phosphorylation and transport of
                     sugars across the cell membrane; protein IIA transfers a
                     phosphoryl group to IIB which then transfers the
                     phosphoryl group to the sugar; IIC forms the translocation
                     channel for the sugar uptake; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAT7995345.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AVLLIITRKQKNQRKIH"
     gene            2903..5518
                     /locus_tag="JA154_05660"
     CDS             2903..5518
                     /locus_tag="JA154_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAT7995346.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKQEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            5778..6932
                     /locus_tag="JA154_05665"
     CDS             5778..6932
                     /locus_tag="JA154_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAT7995347.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKDTIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(7055..8968)
                     /locus_tag="JA154_05670"
     CDS             complement(7055..8968)
                     /locus_tag="JA154_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAT7995348.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     DLFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            9210..9527
                     /locus_tag="JA154_05675"
     CDS             9210..9527
                     /locus_tag="JA154_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT7995349.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            9550..10782
                     /locus_tag="JA154_05680"
     CDS             9550..10782
                     /locus_tag="JA154_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT7995350.1"
                     /translation="MNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMVFVGSL
                     VTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVAGLAGA
                     GLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGEDSALP
                     DFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFVLLNFL
                     VVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWITLGGL
                     GATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPMWIGTV
                     VISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFTVNQDVRGLIVLAINIVLLFMIWY
                     PFFKAYEKEEIKLESQEV"
     gene            10811..11164
                     /locus_tag="JA154_05685"
     CDS             10811..11164
                     /locus_tag="JA154_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAT7995351.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            11186..12631
                     /locus_tag="JA154_05690"
     CDS             11186..12631
                     /locus_tag="JA154_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HAT7995352.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKKRYKKDSFYWYQNVIRTNGEELKEK"
     gene            13023..13775
                     /locus_tag="JA154_05695"
     CDS             13023..13775
                     /locus_tag="JA154_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT7995353.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTVNKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            13877..14797
                     /locus_tag="JA154_05700"
     CDS             13877..14797
                     /locus_tag="JA154_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAT7995354.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICENKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            14800..16716
                     /locus_tag="JA154_05705"
     CDS             14800..16716
                     /locus_tag="JA154_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HAT7995355.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGL
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            16736..17605
                     /gene="kbaY"
                     /locus_tag="JA154_05710"
                     /gene_synonym="agaY"
     CDS             16736..17605
                     /gene="kbaY"
                     /locus_tag="JA154_05710"
                     /gene_synonym="agaY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089588.1"
                     /note="catalyzes the reversible reaction of
                     dihydroxyacetone phosphate with glyceraldehyde 3-phosphate
                     to produce tagatose 1,6-bisphosphate; in enteric bacteria
                     there are two D-tagatose 1,6-bisphosphate-specific
                     aldolases: KbaY (also called AgaY), involved in catabolism
                     of N-acetyl-galactosamine and D-galactosamine, and GatY
                     which is part of the galactitol catabolism pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HAT7995356.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            18114..19577
                     /locus_tag="JA154_05715"
     CDS             18114..19577
                     /locus_tag="JA154_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HAT7995357.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIVDGLEELERMLEV"
     gene            19581..20741
                     /locus_tag="JA154_05720"
     CDS             19581..20741
                     /locus_tag="JA154_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAT7995358.1"
                     /translation="MEYRLNEVFEFIRNGASIKQFDTKFGYPITRIETIANSYIDTNK
                     MGYADIKELGKLEDYLLKNGDILMSHINSEKHLGKSAIYEDINKKIIHGMNLLCLRPD
                     KEVCYPKYLNYFFNSNEFRRQLPRIIKKSVNQASFSVNDLKNLKVDLPEMEAQKDISK
                     VLDKAQELIDKRKEQIEALDELVKSRFIEMFGNFKEQKFVLLKDCTNFIDYRGKTPIL
                     SENGIRIINAKSVGNGVFKYINEYISENTYNSWMKRGFPVAGDVLFVTEGHTFGNVCR
                     IPKDLKKFAMGQRIITIQGDKKILNNAFLAQYMQTKAFRVEIDKYKTGSSAQGIRSKE
                     LQKILIPVPSIELQNKFEEFVNQVDKLKVGIKTSLKELEDNFNSLMQKAFKG"
     gene            20786..21094
                     /locus_tag="JA154_05725"
     CDS             20786..21094
                     /locus_tag="JA154_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995359.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            21127..21507
                     /locus_tag="JA154_05730"
     CDS             21127..21507
                     /locus_tag="JA154_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAT7995360.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYKSKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            21497..21925
                     /locus_tag="JA154_05735"
     CDS             21497..21925
                     /locus_tag="JA154_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995361.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            21975..25310
                     /locus_tag="JA154_05740"
     CDS             21975..25310
                     /locus_tag="JA154_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAT7995362.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKTETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            25691..>26726
                     /locus_tag="JA154_05745"
     CDS             25691..>26726
                     /locus_tag="JA154_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAT7995363.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVL"
BASE COUNT        10586 a         2774 c         4936 g         8430 t
ORIGIN      
        1 aaaaatatga gtataaattt acagatacag aagacatgat aattgaatat atgagaaaaa
       61 acaaagaaga aatagtaaaa ttatcaatac aatctttagc agataaattt tttactgtcc
      121 caaataccat agttaggcta tgtaaaaaaa tggagtatga tggtttctct caactaaaaa
      181 ataatttaaa agaagagtta aaaaatgagg ataaagaggc aaaaaatggt tttgaatcag
      241 atataaaaaa gacattagag ctaatagatt atgaaaaaat aaataaagta gcagatataa
      301 ttcaaaaatc taggagtatt atagtttatg gaataggtca aaacattcct ttatgtgaga
      361 tattagtaaa agaaattaga ggtgggcatg ataatataac ttactatagt caaagacatg
      421 aagtacttca tgatatagga aaattaggtg aacaagatat gttttttgca cttagtttaa
      481 gtggagaaac gaaagaagtt atagaagtag caaagacagc agttaataag ggttgtaagg
      541 tagtagctct aacacatata tgtgaaaatc aattgcaaca aattgcagat ataagtttat
      601 attgctattc aacaattaaa caagtaaatg ggtacaatat atcagataag gttccaatta
      661 tgatgattat gagagcaata atagagcaat actggttaag atagagattt gtgtataaat
      721 acacaaatct ttatttttta agaagaaatt gtgtgtataa tttcaaaaaa acattttcat
      781 aatttatttt atgctatatt catagcatta aatgatttaa ggagggcgta attttggaat
      841 ttaagaaaat cacaaatgaa aaccttgttt ttttaaaaca aaaaataaaa acaaagaaag
      901 acgcaataaa gttcttagca agaaagctat atgatgaagg caagttaaca tcttatgagg
      961 aatatttaga agctgtaatg tacagagaaa ctttatctcc aacaggtttt gaaagagggc
     1021 ttgcaattcc tcatggaaaa tctaagtcag ttaaagaagc tgcatttgca gctatgacct
     1081 tagagacacc aatagaaaat tgggaaagcg tagacccaaa taataaagta gagataatat
     1141 ttttaatagc agtaccagaa aatgaaggtg gaaatacaca catacaaata ctttctgagt
     1201 tagttactag actgtcaaat gaagattata tgaatatgtt gttaaattca aaaactaaga
     1261 aagagttatt taataactta gatataaaag aaaagaaaga acaaataaaa gaagagatta
     1321 ggattcaaaa tgaatcaaaa aaaattatat tagcaataac agcatgccct gcaggtatag
     1381 cacatacata tatgtcagca gaagcattaa ttaaagctgg taaagaaatc ggtgtggatg
     1441 tgtatgtaga aaagcaagga gctaatggta tggtggaccc tcatacacca gatataataa
     1501 aaagggcaga tgcaataata tatgcaacag atgtagcacc taaaaataca gaaagatttg
     1561 aacatttgcc aaacataaaa acgtcagtag cagctccact aaaaagagca aaagaaataa
     1621 tatacgaagc tttagaagta gcacaaaaac aaggtaaagg tgattatata gaaaaatcat
     1681 ctgatatatc atattgtgaa aaatcatcat ggaaaaaaga tgtaaaatca gcaatactta
     1741 caggagtatc tcatataata ccacttattg tggcaggagg tatgatactt gcatttgcag
     1801 tattagcatc tcaagcattt ggtctacaag aactatacaa tcaagaaggt tcttggttat
     1861 ggcaattaag aaagctttct ggaggtatgt taggtacatt gatggttcca gtattatcag
     1921 catatatgtc ttactcacta gctgaaaaac caggtcttgg accaggattt gcagcaggtc
     1981 tagcagctga tttaataggt agtggatttc ttggaggtat gttaggaggt ttacttgctg
     2041 gttatatagt aaaattttta aagaagaata taccagccaa aggcacatta gctggattcg
     2101 taagtttttg ggtatatcca gtagttggtt ctttggtagt aggtttattg atgttatttg
     2161 tagtaggaaa gcctgtagca gcacttaaca atggtcttat aaattggtta aatgggatga
     2221 ctggagtaaa tgcaatacta ctaggtgcaa taataggagc tatggtttca tttgacttag
     2281 gtggaccaat aaataaagca gcctatacat ttgcaatagg agcaatagca agtggaaatt
     2341 ttatacctta tgcaatattt gcatcagtta agatggtttc agcttttgga gttacaatag
     2401 ctactgtttt aggcagagat ttattttcag aatcagaaaa ggaaatagga aagcaaactt
     2461 ggatattagg attggctgga ataactgaag gtgcaatacc atttatgata gaggacccaa
     2521 tccgtgttat accatcattg gtagctggtt cagccataac tgggtctata gtagcattct
     2581 tcaatatagg tcttcaagtg cctggagcgg ggatattctc attggcgctt ttagagggaa
     2641 gtatgtcagg atttattata gcatcaatat ggttctttgc ggcagtactt ggagctataa
     2701 tatcagcagt attattaata ataactagga aacaaaaaaa tcaaagaaag atacattaaa
     2761 agatacgtta ttagatattt aaatcaaagg tttaattata gtgataaaat aaatattaaa
     2821 aaattttgta aactcagtag tatttttatt ggatttttaa ttagacagaa aatattatca
     2881 gataagtttt ggaggataag caatgaaaaa aaatgtacac atagttcctc atatgcattg
     2941 ggatagagaa tggtacttct caactgagga atctagaata ttacttgtaa ataatatgga
     3001 agaaataatg gatatgttag aaaataatca taactatcca tattatgtga tggatggtca
     3061 aactgctata ttagaagatt atttagcagt taagcctgaa tgtaaggaga gaataaaaaa
     3121 attagttcaa gaaggaaggc taataatagg tccttggtat actcaaactg atgaaatggt
     3181 agttggtgga gaatccatac ttagaaattt actatatgga ataaaagatt gtgatgaatt
     3241 tggagaatat atgaaaatag gatatttacc agattcattt ggtcaaagtg ctcaaatgcc
     3301 acaaatacta aatgggtttg atataaaata ctcaatgttt tggagaggat gttctgaaag
     3361 aaaaggaact aataaaacag agtttaattg gaagagtgat gatgggtctt ctgttttggt
     3421 tcaaatactt ccattaggtt atgcaatagg aaaatatcta ccaatgaatg aagatgagct
     3481 aagaacaaga atggataaat atttaccggt tcttgataag ggtgcaacta ctgaccatat
     3541 aatacttcca aatggacatg accaaatgcc aattcaaaag aatatatttg aagttatata
     3601 taaattaaaa gaatgttatc cagagagaaa atttttctta agtagatatg aaaacatatt
     3661 taaagagcta gaaaaaaatg aggatataga tacaataaaa ggggaatttt tagatgggaa
     3721 atatatgagg gttcatagaa gtatattctc aacaagaatg gatataaaat cagcaaatgc
     3781 tagaatagaa tcaaagataa ccaacatatt ggagccacta gcttctattg catattctct
     3841 aggatttgaa tatcatcatg gattaataga gttaatttgg aaagaaataa tgaaaaatca
     3901 tgctcatgac agcattggat gttgttgttc agataaagtt caccatgaaa taatgaatag
     3961 atttttctta gctgaggaaa aagtagacca attaataaca ttttacaaaa gaagaatagt
     4021 tgatgcaatg agttgtgaaa tggctttaga taagttagta gcatttaact taatgcctta
     4081 cgataggaat gaaatagtaa gcgctcaagt tataactaaa atgaaagcat ttgaaatatt
     4141 tgataaagat aataataagt tggattttga agtggtacat aaagaagtaa tagatgcagg
     4201 attaatagac cgtcaaattg ttcattatgg aaactatgat tcatttatgg tatatacaat
     4261 aaactttaaa gataaaatac ctgcaatggg gtataaggcg tatatgataa aagaagttga
     4321 acatatgtta gaaaaagctt atgaagtatg tgacatggta gataatgact tttatactat
     4381 tcaagttaat gaaaacggga cattaaagat atttgataaa aaattaaata aaacttttga
     4441 aaatgtatta ttgatggaaa atggaggaga tgaaggagat gagtatgatt tttcaccact
     4501 tccaaatgag aaattaatat ttaacgataa tatagtagca aattccacta taaagaaaaa
     4561 tagatttaat aatgagataa agataaatta tagattaaaa gtaccaaaag atatacaagc
     4621 tagaaaaaat aatagagcag attcatttat tgactttgat atagttataa atgttccaaa
     4681 tgataaagct ataatagatg tagtattcaa cataaataac caagcaagag attgtagggt
     4741 tagaacttat ataccaacaa atatagcttc aaagttttct gtgtctgata atcagtttgg
     4801 acatataaag agaagtgtgt atgatgatgc tatggaagtt tggcaacagg aagaatggag
     4861 tgaaagacca gattctatat atccaatgct aacattcgtt ggattgtctg acaaagagca
     4921 tggagtagct gttcttacaa acagtacaag agagtttgaa atagtagggg aaaaatttga
     4981 cacaatagct ataacattat ttagaagtgt aggattccta ggtaaagaag aaatggcaag
     5041 acgcccaggt cgtccatcag gaataaagtt gcctactcca gattctcaaa tgataggaga
     5101 tataaaaata gacttagcaa tagttactca tgaaaaatct acactagaag caaatgttgc
     5161 aaatatggct aaagagtatt taacaaaaat acaaacttat aataaaatgc catataatgc
     5221 tatgaaattg aatcaatcag aaatagaagt agattattca tatagtttac ttaaggaaac
     5281 taataaggac cttgtgttaa gtgttgtaaa aaaggctgaa aaacaagagg cattagtagt
     5341 tagattttac aattctaaag aagaaacaaa atcagctaac atagtattta acaaaaatat
     5401 taaaacagct aaatggatta acctaaatga aaaagaatta gaatctatat caatagataa
     5461 taactctgta aatgtagagt gcttaatgaa ccaagttaaa actatactgg ttaaatagtg
     5521 ctaaaaagga attatcttaa aatagagata atcctcctta tcattgttga aataataaac
     5581 atatatttga ttttatacaa aaagagtgtc tcatgaacta agaaatttat tttgagacac
     5641 tcccttttta taagataaaa gaatattaat ggtataagtt gtatacaaag agagatgtag
     5701 agtataaaat aaaagataaa ttaagcttat aaaattatat gtttaaaata aggagtaata
     5761 aaaaggagta gataaatatg aaaatattaa tttctataga ttctttgaaa ggaagcttat
     5821 cttcaataga ggcaggaaat gcaataaaaa aggggatatt gaaggttaaa gaagatgcgc
     5881 aagtaaagat attgccactt gctgatggtg gtgaagggac agtagatgcc ttagttcaag
     5941 ggatgaatgg taaaaaagat actatagaag ttactggacc aatagctaaa aaagtggatg
     6001 caacatatgg attattaaaa aatacaagta cagcaataat agaaatagct caagcatcag
     6061 gactaacatt ggttccgact gaacttagaa atcctttata cactacaact tatggtgttg
     6121 gggaaataat aaaagaagca ataaataaag gctatagaaa ttttatagtt gggataggtg
     6181 gaagtgctac taatgatgct gggataggta tgcttcaagc cttagggttt gagttttatg
     6241 atgaaaataa caagttagta ggattaggtg gaaaagtttt aaatgaaata agacatataa
     6301 aaatagaaaa tagattaaag gaattagatg aatgtaagtt taaaatagcc tgtgatgtaa
     6361 ataatccgct gtttgggaaa aatggagctg catacatata cgggtctcaa aaaggtgcaa
     6421 catcagaaat aattgaggag ttagataatg ggcttagaaa tttttccaaa gtagttaaaa
     6481 attacttaag taaagatgta gctaatgtag aaggagcagg agcagcagga ggattaggtt
     6541 ttgctttttt agcattttta aattcaaaat tagaatctgg aataaaaata atattagaag
     6601 aaattaaatt agaggaagag ttaaaagatg cagattttgt cataactggt gaaggtagat
     6661 tggacaatca aactgctatg ggcaaggctc caataggagt tgcaaaactg gcaaaaaaat
     6721 atggggtaaa agtaataggt ttggctggag ccacaacaga agatgcagtt aaatgtaatg
     6781 aagagggtat agatgcttat ttttctatag taaatagagc tatgacaata gaagaagcaa
     6841 tggataaagc aactgctagt gagaatatga cagctacaac aacacaaata ttcaatctta
     6901 taactagtat acaacagtca aaaaatatgt agtattaaaa caaggtatat taggtaaaaa
     6961 gctgtcacat attaatttag actttttagt gtgacagctt atctatttta tgttaataac
     7021 ttaagaaaca tatattggaa ataaattaat tgatttagat attttgaata agataagcta
     7081 ttctattcat aaaactacta tagttatctg ccttagtaag ttctttaaca ttattccact
     7141 cagataatat ttcaataaag ctttcaaata tgtaattgaa cttttctttt tctttttcat
     7201 ttataggtat catagctata atttgaactt tttcctcacc ccatttaact ggtttatcgt
     7261 taacaattaa acatactccg gtttttaaag catccatttt catgctatga ggaattgcta
     7321 cattgttatt aaatgcagtt gaagacattt tttctcttat aagaacatca tctatataat
     7381 tgggtttgac atatttattt tcgtacagaa tatttcccat aaatcttata taatctttag
     7441 cactatctaa atatatattt ttcataaaca aagattcact aaagtacatt tctaaatgat
     7501 tttgtacagt aagtatattt tttctatctt taatctgaat aaatttactt tgaatattat
     7561 cataatcctt tctatttagg taaggattta cataaacaaa aggtataggg catctatcgc
     7621 taattggtaa agtagtaata accatgtcta ccttattgtc aaaatcaaga ttatgagtat
     7681 ctgaaaaaat ctgaataatt tctatggatt cattaaatct cttctcaagg tctcttacta
     7741 ttttgaattg taaatcatag taatttggag taattaatgc acagagaatt ttatcttcta
     7801 gtaatttttg tctatcaaaa aatgaaccta catgaagaga tatatacgat atttcatctt
     7861 catctatttc tatgttttct aaaatttgaa tctgattaca aatataaaca gatatatcat
     7921 atatcagagg gtaggaatct tttaacttct gaggtatttg atttctcaaa acttgattat
     7981 ttttagccct actgattaaa ttttttacat gaagagtaaa ttttacaaca aattcatcat
     8041 cagataaatc tataaagtat aagtcatata catttttaat tattttttta gttaatttaa
     8101 caaaatgttc atctatataa tttggcaatg acttagtatc catttcatga taatttaaga
     8161 ctgtggtttt gtttgttaac tgaaatacta agtagtataa ttctgcacta gttaaagtta
     8221 tattgtattc agactctaaa aaatttgata tatctcttga aatgcttata tcattattat
     8281 tatctatgta gtttactact tcgacagatt ctatgtagtt attctttttt attctgtcta
     8341 tagttatcat taaatgaaga agtatgttat ttatagcata ctcatttata aacaaattgt
     8401 atttttttaa tatatatatt atattttctt tcagtttggt caaattatac tcttgatata
     8461 ttttttgtac atcaaataag cttaaaaaat cacttcccgt ttctttaaat attatagagc
     8521 tcattaattt gcgctttgca gactcatttc caattagttt caataaaaat ttcgattgct
     8581 ttatctttaa attataactt cctataaatt tattagcttt gactatatct ccttcaatag
     8641 tagctggact aacaaacaag tcttcactta aatcaaatat attaattcca ttactattaa
     8701 ctataagttc ttttaaaatt aaatttaacc tgttatcagg tgatatattt tctgaactat
     8761 caaccttggt ttgacaatta ttattgagat ttactttata tcctttttta gaggataata
     8821 taaattcacc attggaatta atttcgttta catattttct aattgaacgc gttgagacat
     8881 taagataatt agccaatgta gatgaatcta tccaatcatg ttgtgttttt atgaaattta
     8941 aaaatagttc ttttttagat gttttcatta gattccctcg catagtttta aaataaaatc
     9001 tggatgatat gataattata taattgaatg tacgtattat aagatacatt atactatatt
     9061 aatatgttta attaagagta actgatttcc tattattagg aaatatattt atgtgaaatg
     9121 aacttatttg gtaagtacaa tataaacata ataaaataaa aagttcatta agaaataaat
     9181 tgatggataa aaatattgga ggtatcatta tgaaaaatat aagagtgctt ttggcgtgtg
     9241 gttcaggagc aagtacaggt tttatggcaa ctagtatgag aaagagtgca aagaaaaaag
     9301 gtatagaagc agatataaaa gcgagaagtg agtcagagat agataagtat ttatcagaaa
     9361 tagattgttt attactagga ccacatttag attatatgaa ggaagaaatt caacaaaagg
     9421 ctaatgaata taacgttcca gtagcatgta tatctaaaac tgcatatgga agactagatg
     9481 gggcaaaggc tttggaagaa gcactagaac taatatcaaa caaataaaat atttatgggg
     9541 agagtgttta tgaataaatt tatagatttt ttaagtgaaa aatttactcc tgttgtaaat
     9601 aatatgacta agaatatatg ggtacaatca gttcaatcaa ctataatgaa agttttgcca
     9661 atggtatttg taggctcatt agtaactata gtttctgtct tgaagaatta cataagcttt
     9721 ttacctgatt taagtccaat aaatcaatat acatttggac ttttaggatt atttatagca
     9781 tttttattgc ctatggaaat aatgaagaat aagaaatttg aaagtatgag tgtagtcgct
     9841 ggtcttgcag gtgctggatt aatgttaatg atgattcgtc cagagattac gaatgaagga
     9901 gctatattta acttcaacag gtttggagga gaaggaatgc ttgtatcgct agttgcagga
     9961 ttattctctg gattaataat gtccttattt ggttcttttt catttttcgg agaagattca
    10021 gcacttccgg attttgtaag caagtggttt gataatatgt tacctataac agtgataatg
    10081 tttgtaggct ggggattaat ttatggactc aactttgaca tgtttgaaat aatagttaga
    10141 gtatttaaac cagtatcaga tatagcacag acattacctg gatttgtact cttaaacttt
    10201 ttagtagtat ttatatattc tttaggaata agtgggtggg tactatctcc tataacttat
    10261 tcaattcaac ttggtgcaat tgcagctaat gcagcagcag tagcatcagg aggagtagca
    10321 actaatatat atgtttacga agttattcaa acaggttgga taactttagg agggttgggt
    10381 gctacattac cattagttat aatgatgtgt ttatccagag ttggaagaat aaaagcaata
    10441 ggaagagcat gtattgtccc ttctataatg aatataaatg aaccagtaat atttggagct
    10501 ccagtggcat ggaacccatt tttaatgtta cctatgtgga taggaacagt agtaatatca
    10561 ataactactt acttatcaat gagtatggga tttgtgacaa ttccaagtaa ggtgtttaat
    10621 ttatggtatg ttccttttgg tatatctact tttacggtaa atcaagatgt tagaggtctt
    10681 attgttttag caataaatat agtattgcta tttatgatat ggtatccatt ttttaaagct
    10741 tatgagaaag aagagataaa attagagagc caagaggttt aaatttagaa attgaaagga
    10801 aattataaat atgacaattg aagaacatat aatagatgaa gaattagtag aggtggcaat
    10861 gcaaataata cttcgtgcag gtgaggctag aacagaaatt aaacatgctc ttaatgattt
    10921 ggaaaagttt gattatagaa atgctgattt aaaactagct aaagcaaaag ggtttatgac
    10981 agaagcacat agagctcaaa ccaagattat acaaggggaa gctggtggag agaaaagagc
    11041 acattcgctg ttatttgcac atgctcaaga tactcttatg accatattca gcgagttaaa
    11101 tataactatg tctttagtag gaatagtaaa gtctatagaa tataaggtaa ataatagtaa
    11161 atgatttaaa taaataaaat aaggagtgat taaattgggt aagtttccta aaggtttttt
    11221 atggggagga gctgtagcag ctaatcaatt agaaggtgca tggaatgaag gtggcaaagg
    11281 ttggtgttta gctgatgtac atatgtatga taaagataga gatatatcta aaccttatga
    11341 gtctgatatg actatagaaa gaataaagtt tgctatgaaa gataataatg gatattatcc
    11401 taagcgtagt ggaatagact tttatcatac atacaaagaa gatttaaagc ttttgaaaga
    11461 gctagggtta aagtgtttta gattttcaat aaattgggct agaatatttc caaatggaga
    11521 tgatttagag cctaatgaag agggattaaa gttttatgat aatcttataa atgaagtaat
    11581 agataatggt atggaaccta ttgtaacact acttcactat gagactcctt tgaatttagt
    11641 attaaaatat aaaggatggt ctaatagaaa agtggtagat ttctttgtta gatatgcaga
    11701 attaatacta aatagatata aaaataaagt taaatattgg atagttataa atcaaataaa
    11761 tttaatacat tatgaatcat ttaattcagt tgcattttgt gtagaccaag tagataatgt
    11821 agaagagact agatatcaag ctattcatca tcaattcata gctacagctt tgactaagaa
    11881 gcttggaaaa gaaataaatt cagatatgat gataggtatg atgatggcag attgtacatc
    11941 ttatccagaa gattgtgacc ctgaaaatgt agtatttgct ttaaaaagaa ataggctaca
    12001 atattttttc actgatgtaa gttttagggg agagtatcca gtctatatta agagattttt
    12061 caaagaaaat aacataaata tagttatgga ggaaggtgat gataaaatct taaaagaaaa
    12121 tactatggat tttctagctt tatcatacta ttattctaca acagtatcag caagtaaaaa
    12181 caccatgaat ccaactgaca atataaagaa tccatattta aaagcaaatc cttgggggtg
    12241 ggcaattgac ccaaaaggat tatacaatac tttatctcaa tattatgata gataccaaaa
    12301 gccaataatg atagctgaaa atggatttgg aatgcatgat aaatttgaag atggaaaagt
    12361 tcatgatagt tacagaatag attatttagg aaaacatata gaacaaatag gtgaagctat
    12421 agctgatgga accgaaatat tagcttattg tatgtggtca ccaatagata tagtaagttg
    12481 ttcatctcaa gaaatggaaa aaagatatgg atttatatat gtagatatag ataacaatgg
    12541 aaatggaagt aaaaaacgtt ataaaaaaga tagtttttat tggtatcaaa atgtaataag
    12601 aactaatgga gaagaattaa aagaaaagta aatagttttt aaatataaac aacccccctt
    12661 ataaatactg tatatattat taatatagtt ttataagggg ggttataatt gtaaataaaa
    12721 tgatttatta attatatgat tcatagcgaa atttatattt atacttttaa tcataaacat
    12781 atcagtataa ggaatatcta ttgcacttca tccaaatttt ggtagaggta caattctttt
    12841 ttattttaaa atgctataag ataacataat aaactttaag caaaaaatta ttatattata
    12901 aatttacata tatagacatt gactaaatct taattgtata taaaaataag tagttaagag
    12961 gtaatatatt atattactcg ataaatttag acaaataaaa cttatataag gggagataac
    13021 atatgataat tgagctaaac aaaagtaatt ctgaaaatct aaccaagacg gaacttgaaa
    13081 taattaaatt tataaatgaa aatgaatcaa tattaccaga attatctata gtagagattg
    13141 ctcatgaaac atattcatct ccagcaaccg tttcaagagc aattagaaaa tgtggtttaa
    13201 atggattcaa tgaacttagg tatagattaa cagtaaaaga tgaaaatgat gatatacaaa
    13261 atatggggga tataataaat aaatccttta tagaagcaca gagagttata gaacaaatat
    13321 ctttaactac actaatcaat ataataaaaa ctgtaaataa atctagtaag atatatattt
    13381 tagcaagagg gcttactgaa tatgtagcaa aagagtttag tcttaaattg caattacttg
    13441 attttaatgt tgtttttata aatgacccca acataatgaa aataaaatct aaatcaataa
    13501 aaaaagacga acttataatt atattttcat tgaatggaaa tacaatagag cttgtagaat
    13561 cagcacagaa tgccaattta gttggagcta aggttataac ttgctgttgt agtgaaagtg
    13621 caaaacttat aaagtattcg gatttatata ttgtaggata caaacatgaa catatatcaa
    13681 tttctgaata tgaagtatct tccagattgc ccttgtatat tatatctagg ataattatag
    13741 attatatggt cagatataat aattctgaaa attaaattga aaagtctatg aaaatcgatt
    13801 ttctatttgt gaaaagattt tcatagactt ttttattata atgaaaacat acacagcaca
    13861 agcaaggggg aagacaatga tatatacatt aactacaaat cctgcaatag atatgaatat
    13921 atctactaat gggataaaga gaaaattagt aaatcgtaca tcaaatgctg tatattctcc
    13981 taatggaaag ggattaaatg taacttttgt actaggtcat tatggtatag aatctaagat
    14041 attaggattt tttggaggat tttcaggaaa atatatagtt gaagaaagcg aaaagagagg
    14101 atttgacgta ttgcctactt gggtagaaga tacaactcgt ataaatatat ttttaaatga
    14161 tggaagtgat gaatttaaat ttgtaaactc tgggtcatat gtaaacgaaa aacaaaaact
    14221 agatatgcta gataagatac agtcattaga agatataagt tacttatcaa taagtggaag
    14281 tctacctcct ggtattgatg ataattacta tgaggatata tttaaaattt gtgaaaataa
    14341 aagtataaag acaatactcg atataagttc accaaaatta aaggagttac tacagtataa
    14401 tccatatttg ataaaaccaa atgatgaaga aatcaaagat atatttggaa ttattgtaag
    14461 agatgaagaa gatattaagg atgtacttaa attactacat ttaaaaggtg ctcaaaatat
    14521 attgctaact ttaggagaaa aaggttctta tttttataat ggaaaatcaa tttattatgc
    14581 tagtgctcaa ccagttacag tagtcagctc tgcatgtgct ggtgattctg cattagctgc
    14641 ttttttaagt atatggttgg aaaatccaga acatattgaa gaagcattaa aaaggtcagc
    14701 agcaacagga gcaagtgtgg ctgaaagtaa tggtataggt aatttggaga atgtggaaga
    14761 ttatataaaa aatataagag tcagaaaggt ggtataaaaa tgggaaagaa aatgattcta
    14821 ggggtaacag gttgtccaac tggaatagct catactttta tggcagaaga agcattaaaa
    14881 aattcagcca gggaattagg gtgtgacata aaagttgaaa cgaatggagc tataggtgta
    14941 gaaaataagt taacagcaaa agatatcgaa atggcagatg caataatagt tgcttgtgat
    15001 aaaaatgttg atatggacag attcaatgga aagccagtaa tagaagtacc tgtcaaagaa
    15061 ggtatacata aagctaatga acttatacaa agatgtattg atggaaaagt agctattaga
    15121 aaaggaaact tatcaagcag taaaattgca gaagaaggaa atttatcgtt tggtcaaaag
    15181 ttgtataaag acttaatgaa tggtgtatct cacatgttac ctttagtagt agcaggtgga
    15241 gtacttactg ctatatcatt tttatggggg atttattcat ttgacccaaa ttcagaacaa
    15301 tataatcaaa tagcagctac attaaaatct gttggtggat attctatgaa cttgatggtt
    15361 cctgtattgg cagcatttat agctcaatct atatcgggaa gagctggtat gttggcagga
    15421 cttgttggag gaatgatatc atttgataca ggttcggggt ttttaggtgg aatcatatca
    15481 ggatttttag caggttactt agttaaacta tttgtacatt tgttaagaaa attaccacga
    15541 caattagaag gtcttaaatc gatttttata attcctattg tcagcgtagg tcttgtgggt
    15601 gtagcaatgt tgttacttgg tgggccatgt tcagcactta acaatgctat gatgaacttt
    15661 ctatcggggt tacaaaattc aagccctata attttgggaa tagtaatagg atgtatgtca
    15721 gcatttgata tgggaggtcc agtaaataaa gcagcatatg taactggaac tatgcttcta
    15781 ggacagggaa attatttatt tatggcaggt gtatctgcag cttgtataac accaccattg
    15841 gtaatagcaa tagcatctac tttatttaaa aatagattta cagaagaaga tagagcagca
    15901 ggattaatca attatatact aggtagtact catataacag agggggcaat accttttgca
    15961 gcaaaaaatc cattaaaagt gttgccagta cttatgattg catcatcaat atcatcaatt
    16021 ttaacttata taatgaaaat agaagtacca gctccacatg gagggttttt ggttctagga
    16081 ttagtcaata agccactttt atgggtaggg tgtatatttg taggttcatt agtaggtgct
    16141 gtactttata tgatggttac acctaaagta gtaaatgaga aaaatactat agaaaataca
    16201 aatgtaacaa aagatgaaca taaaaatgaa agtgcagaat cggaaaacgt atctaataat
    16261 atctctttat atagcgaaga aacagtaatt ttagatgtta agggaaaaaa taaaacagat
    16321 gtaattgatg aaatgattgg aatacttgat aaatctagtg ttcttttaga taaaaagaag
    16381 tttaaagaag aaatccttaa aagagaagaa atatcatcta caggttttgg tatggggata
    16441 gcgataccac atgcaaaaac agatgcagta aaggtaccac gtgtagcagt aggtctatca
    16501 aaagaagggt ttgactttga atcagaagat ggaagcttgg cacatattat atttatgata
    16561 gcagctacag ataatggtga taatttacac ttaaaaacat tatctcaact atcttcaaaa
    16621 ttaatgaatg aagaattttt aaatgagtta ttaaatagca aaacaagtag agaaatagta
    16681 tcaaaattaa ataacgaaga aattaaatca gtataaacat ataggaggag aaattatggc
    16741 attagtgaca actaaacaaa tattattaga tgcacaagaa gggcattatg cagtaggtgc
    16801 atttaatgtt gaaaatatgg agatggttat ggcagtaata gaggctgctg aagaacttaa
    16861 atcaccagta atattgcaaa caacaccttc aacaataaag tatgcaggac tagattatta
    16921 tttagcgaat gtaaaggtag catcagagaa ggcaagtgtt ccagtagcaa tgcacttaga
    16981 ccatggttca agctttggac tagctatgca ggcacttaga gttggatata cttctattat
    17041 gattgatggt tctcatgaaa gttatgaaga taatatttca atatctaaat ctgttgtaga
    17101 tgcatgtatg ccttcagata tacctgttga agctgaatta ggtaaagttg gaggtaagga
    17161 agacgattta gatggtggtg atggtggagc atatacagac ccattagaag caaaagagtt
    17221 tgtagagaga acaggggtat catctttagc agtagcaata ggtacggcac atgggctata
    17281 taagggagag ccaaaacttg atttagatag attatctgaa attagagagg ttgtatcagt
    17341 tccattagta ttacatggtg ggtctggaat accagatgag gttattaagg agtctataaa
    17401 aagagggatt tgcaaggtta attatgcaac tgaattaagg attgcttata gcaatggggt
    17461 taaggatgtg cttaattctg acccagaggt aattgaccct aaaaaatatg gtaaaaaagg
    17521 tttagagtca gttaaagatt ttgttaagag taggatggaa gtttgtggat gtgtaggaaa
    17581 agcaatagag atagtgaagt gttaataata aagggaaaga ataagtaaga ttaaattaag
    17641 gggtgtctca aaatgaactt ttagttcatg aggcactctt ttttgtatga aattaaatat
    17701 attttcatca ttccgacaat aaaaaagaag attatatcta ttttgagaca gcatcttatt
    17761 ttattttttg attaagattg taattgagaa atttgaaata aggaagatga tataattaaa
    17821 taaaaaattt ctttctaagt ttaaattaat attaaaaagt tataatatgg taagagttat
    17881 ccaggtagat gacttctatg atggatatat gataaaataa ttttgtatgg tatatataat
    17941 aatcataaaa taagagtatt gcattagttt tttagctaaa atatgtaaat aggaaaaata
    18001 atttaataat tgtgtagtaa aaacttggat ttaacataag acactaacta ttaaggcgta
    18061 cgactaaaat atatatttaa attattttat gaacactgga gggaaagtca tatatgatta
    18121 caggagaatt aaggagtaaa gtggacaaga tttgggagac tttttggact ggtggattaa
    18181 ctaatccatt agaagttata gaacaattta catatctttt atttattaaa ggattggatg
    18241 aagtagaaac aaaaaatgaa gcagaagcaa ctcttttggg tgtttctttt gaaaagatat
    18301 ttgatgatga acatcaacat ttaaggtgga gtaagttttc taatgaagga aattcagaaa
    18361 aaatgtatga aatagtacaa aatgaagtgt tccctttcat aaaaaaatta catggaaata
    18421 aagaatcagc ttatgcaaag tacatgggag atgcaatatt taaaattcct acgcctctta
    18481 tgttatctaa aatagttgat ggtattagta atttagaatt aagtaaggat agagatacaa
    18541 agggagattt gtatgagtat ttattatcca aagtggctac agcaggtact aatggtcaat
    18601 ttaggactcc aagacacata atagatatga tagtaagact tattaaacca acaccagaag
    18661 atattatagt agacccggca gcaggttcgg caggtttttt agtatcatca caacaatact
    18721 taagggataa tcattcaagt cttttcttag tgcaagggct gaaggagcat tttaataatg
    18781 gtatgttcta tggatttgat atggatagaa ctatgcttag aataggtgct atgaatatga
    18841 tgttgcatgg cgttgacaat cctaatatag agtataagga ttctttatcg gaggttaata
    18901 cagataaaga taagtttaca ttggtgttag caaatcctcc ttttaaggga agtttagatt
    18961 atgaagcagt gtcagcagat ttattaaaag tgactaagac aaagaaaaca gagttattat
    19021 tcttggcact ttttttaaga gttttaaaaa ttggtggaag atgcgcatgt atagtgccag
    19081 atggagtatt gtttggttca acaggtggac ataaatcgat aagaaaagaa atagttgata
    19141 atcataaatt ggaagctata atatcaatgc caagtggagt gtttaagcct tatgcaggag
    19201 tatctacagc tataatgata tttactaaaa caggaacagg tggaactgat aaggtatggt
    19261 tctatgatat gaaagctgat ggttattctt tggatgataa gagaaatgag ataaaggata
    19321 gtgatataga ggatattatt aagagatttg ataatcttga tggtgaagta gatagaaaaa
    19381 gaacagagca aagctttttt gttgatgtgg aagagataag agagaatggg tatgatttat
    19441 ctattaataa gtataaagag gtagtgtatg aggaagtagt ttatgatgct cctagtgtta
    19501 ttataggtag agttagggag ttagagaagg aaatcgtaga tggattagag gaattagaaa
    19561 gaatgcttga ggtgtagagt atggaatata gattgaatga agtttttgaa tttattagaa
    19621 atggggctag tattaaacaa tttgatacaa aatttggata tccaattaca agaatagaaa
    19681 ctatagcaaa ctcttatatt gatactaata aaatgggata tgctgacata aaagagttag
    19741 gaaaattaga agactattta ttgaaaaatg gagatatact aatgtcacat attaatagtg
    19801 aaaaacattt agggaaaagt gcaatatatg aagatataaa taaaaaaatt atacatggaa
    19861 tgaatctttt atgtttacga ccagataaag aagtttgtta tccaaagtat ttaaattatt
    19921 tttttaatag taatgaattt agaagacagt taccaaggat aataaaaaaa tcagttaatc
    19981 aagcaagttt ttcagttaat gatttaaaaa atttaaaagt agatttacct gaaatggaag
    20041 ctcaaaaaga tatatcaaaa gttttagata aagctcaaga gttaatagat aaaagaaaag
    20101 agcaaataga agcattggat gaattagtta aatcaagatt tattgaaatg tttggaaatt
    20161 ttaaggaaca aaagtttgta ttattgaaag attgtacaaa ttttattgat tatcgtggta
    20221 aaactccaat tctttcagag aatggaatta gaataataaa tgcaaaatct gtagggaatg
    20281 gtgtatttaa atatattaat gaatatatat ctgaaaacac ctataattct tggatgaaaa
    20341 gaggttttcc agtagctgga gatgtacttt ttgttacaga aggtcataca tttggaaatg
    20401 tatgtaggat accaaaggat ttaaaaaagt ttgctatggg tcaaaggatt ataacaattc
    20461 agggagataa aaaaatttta aataatgctt ttttagcaca atatatgcaa acaaaagctt
    20521 ttagggtaga aatagataaa tataaaactg gtagctctgc acaaggaatt agaagcaaag
    20581 aacttcaaaa aatattaata ccagtaccat caatagaact tcaaaataag tttgaagagt
    20641 ttgttaatca agtagacaaa ttgaaagtag gaataaaaac aagtctaaaa gaactagaag
    20701 acaactttaa ctcattaatg caaaaagctt ttaaaggata gttattcaac taaaatacaa
    20761 ttattaaaat gggggaataa aatctatgaa tcaaatagag ctagatttat accataacat
    20821 ggtaaatatc tataaagaag ctgatttaca atgtaactat aagccaacaa gatttttaca
    20881 actgataaat aacaaaggag ccttattagc tgctaaagaa ttaatcaata aagaaggagc
    20941 tactgaagga tttacaaggc tatgggagtg taagagatta gatttatcat tagaagtatt
    21001 agtattaaaa gaaaagtata aagagttgtt tactgatgaa gaaagagaaa tatgtataaa
    21061 tagattaaaa gattatggat ataaattcaa ttaagcaata ttacctaata aacaagaggt
    21121 gattaaatga gaaatatacc aaatgagatt aaaaactcaa taataaatgt atttccaaaa
    21181 gaaaatactc tagcaatcat aatatttggt agttatggaa cagataaaca tacatcagaa
    21241 agtgatatag acatagcttg gattcccatc aaaaaagtac caattacaga acttgcaact
    21301 aagacagaag ctctaagaaa tatattagac attgatgtag acctaaaaat tgttacagat
    21361 aattatacaa tagcacttaa aaaagctatt ttagaaggtg aagttattta taaatctaaa
    21421 gattttaagg attatataaa taaattctat tttgaaaaca gtgatgtaat agatatctta
    21481 gattggaggg acatgtatgg taattagaaa caaattatct tggaactcaa tagaagaagt
    21541 aaatagggat tttggttctt gcttgtatga cctgcaaaac ttcaaaatct tatatgataa
    21601 ggaagaaatg cctgagttat gggctagata cataaaagaa ggctttaaaa gctatatggt
    21661 atcatttcta gaattaacaa aagcaatgtt gtattataaa tcaattgatt taaacataaa
    21721 atctaaaaac ttttatgact accttttagc ttgtgagtat cacaatttat tacctaaaaa
    21781 ttcttctata gttatagaaa cacttagaaa attgagaaat gatgattctc atggatatga
    21841 tataccacag tttgaagata tgtacgaatt gtttacagaa aatgaagaag tatttgttag
    21901 tataagaaac agctgtaaga aatagttttt agagagattt attttaacta agggacaaaa
    21961 gggggataaa agccttgggt aattttgatt ttttacaaaa gaagaaaaat ttcaatagtt
    22021 ttaatactgc ttgcttagaa gcagaaaaaa gtatactagt tagcccatct acttgtgcaa
    22081 ctataacaag aagagcttta gagcttgcag taaaatggct atatgctaat gatagtgatt
    22141 tagtattgcc ttatcaagat aatcttagta cgttgattca taataatagt tttatagaac
    22201 ttatagacta tgatatgtta cctttattaa aatatatagt taaattaggt aatttatctg
    22261 tacatactag tgcgaacata gagagaggag aagcaatact ttcacttaac aatttgcatc
    22321 aatttgttag ttggatagac tattgttatt cagatgaata tacagcagaa gactttaatg
    22381 aagatttatt acttcatggg gaagagaaaa ggacgagacc agacgaactt aaaaatcttt
    22441 atgagagatt aagttcaaaa gataaaaagc ttgaagaagt aataaaagaa aatgaggaat
    22501 taagaaagtc actaactgaa aagagaaaag ttaatactgt aaactatgat ttcaagatag
    22561 acgaagtaag cgaatttgag acaagaaaaa gatatataaa tatagaatta aagcttgcag
    22621 gttgggaatt tggaaaagat atatgggaag aaatagaagt acaaggtatg cctaatgaaa
    22681 gtggtgtggg atatgtagat tatgttcttt atggtgaaaa tggtaagcca ttagcagtag
    22741 ttgaggcgaa aagaacgagt aaagacccga aaattggtca acaacaagct aaattatatg
    22801 cagattgttt ggaaaagaga tatagtcaaa gaccggtaat atttttgact aatggttttg
    22861 atatgtatat ttgggatgac tattcagata ggaaagttta tggattttat aaaaaatcag
    22921 aattacaact tatgatagat agaagaaaga gtaagaagtc tttaagttct gtaacaataa
    22981 atgatgaaat atccaataga tattatcaaa aagaagctat tagggctgta tgtgaggctc
    23041 ttgaaaataa gcaacgcaaa actcttttgg tatgtgctac tggtacagga aaaacaagaa
    23101 ttgctatatc tatagttgat gtactttcaa gacataattg gattaaaaat atattatttc
    23161 tagcagatag aaaagcattg gtaaagcaag ctaaaaaaag ttttactaag ctattaccta
    23221 acttggcgtt atgtaattta ttggatagca aagacagccc agaagatgct aggatgatat
    23281 tttctacata tccaacaatg atgaatgcaa ttgatgatac gaagtctaag gatggaaaga
    23341 gattgtttac acctggtcat tttgatttaa ttataataga tgaatcgcat agaagtattt
    23401 ataaaaagta taagtcaatc tttgattatt ttgattctta cttaatgggt cttactgcta
    23461 caccaaagga tgagatagat aaaaatactt attcagtttt tgacatggaa aatggagtac
    23521 ctacatatgc ttatgagtat aataaggcag tcgaggatgg atatttagta gattatacta
    23581 gtattgagtt taagactaag attatggaag atggaattaa gtatgatgag ctttctgatg
    23641 aagagaagga agaatatgaa aatacattta atgatgatga gagtattgga gacgaaatag
    23701 ggaataatgc agtaaatgag tggctattta attctgacac aatagattta gttttaaata
    23761 agctaatgac agaggggttg aaaatagaag gagaagaaaa gattggtaag actataattt
    23821 ttgcaaagaa tacaaagcat gctagggcaa tagttgaaag atttaataaa ctgtatccaa
    23881 agtatggtgg gaactttgta aaagcagttg actatagtat aaattatgta gattctataa
    23941 tagatgattt ttctgataag aataaattac ctcaaatagc agtttcagta gatatgcttg
    24001 atacaggaat tgacatacct gaaatactaa atcttgtttt tttcaagaaa gtgagaagta
    24061 agactaagtt ttggcaaatg ataggtagag gaactagatt atgtccagat ttattaggtg
    24121 ttgatatgga taaggaaaga ttcttaatat ttgatttttg taataatttt gaatttttta
    24181 aatttaatcc taaaggattt gagggaaata aaacagaaac acttactgaa aaattattta
    24241 atataaaggt aagtatggtt aaagaacttc aagatattaa atatatagaa ggtgagtatg
    24301 cagaacttag aaaagaattg ttagaagaac ttataacttc tgtaaaagct ttaaatgagg
    24361 atagttatat agttaggatg aatttaagtt atgtacataa gtataaaaat gaaaatgttt
    24421 ggagcaatat tggagcagta gcacaaaatg agataagaga atatatatca ccactcataa
    24481 cttcatatag tgatgatgaa ttggctaaaa gatttgatgt agttatgtat aatatacaac
    24541 ttgcatactt acaaaataat aatgcaagta aaggtataag gcatgtaatg gctacagcag
    24601 aaaaactttc aaaacttggg actataccac agattcagca acaaaaatat actattgaaa
    24661 aagcaatgac agaggatttt tgggaagatt cagatatatt tgaggtagaa gaagtacgta
    24721 tatcattaag agagcttatt aagtatcttg aaaagtctag tcaaaagata tattatacaa
    24781 gttttgaaga tatgattgta gcagaagata gaaatgattc tgtatataat gcaaacaatc
    24841 ttaagaatta taaaaagaag gtagagtatt atttaaatag ccataaagat gaattagcaa
    24901 tatttaaatt aagaaataat aaaaagataa caaagcaaga tgttgaaacg ttggaggaaa
    24961 tactcttaaa acagttaggt aattgtgatg attataagaa ggagtttgga gatactccag
    25021 taagccaatt agtgagaaga ctagtgggat tggatagaga agctgcaaat gaagcttttt
    25081 cagagttttt aaataataag agctttaata ctaagcaaat acattttgtt aaacttatag
    25141 tcgattatgt agtcaagaat ggatttatag aggataacaa agtgttgatg gaagacccat
    25201 ttagaactgt tggaagtata attgatttat ttgaaaatca tattgaagag agaaataaat
    25261 tgattaaaac tataaataaa attaaagaaa atgcatcaga gataggctaa tgatgttatt
    25321 tagtgcaaaa ttattgttaa tgatgtagag aataaattta ttagtaggta gtctaatatt
    25381 gggttgaaat agataactag atatggataa tgtaaatata caaagagagg tattgaataa
    25441 ttatgcaata tctcttttta attgataaat aactatgaat tactcatgaa gaagattaaa
    25501 taataatttt taaaattaag tatttgtata ttttatagaa taatcatgta tttactttaa
    25561 taaaataaaa acaggtataa aaaggtatat aataaggctt tcatatacat ttatgagtca
    25621 aaattttgat ataatatata gatacatgga gttatgaatg gaatttattt aacttatagg
    25681 gggagttaat atgaaagcag gagaaattga atttcttagt tatttagagg ggtcaaacaa
    25741 aagttttgta gtacctgtat atcaaagaaa ttataactgg aagaaagaac aatgtaaaag
    25801 attgtttgat gatttagagg atataataga ttctggattt agaactcatt tcttagggtc
    25861 agttgtatct atatatagta tgggaaaaga atatttaata atagatggac aacaaagagt
    25921 gactactgtg tcaatattat tgattgccat gtgtcaaata ataaaagagt ttggggaatc
    25981 aaaaggaatt attacaacag aagaacagat taaggagtgt tatcttataa acaagcactc
    26041 aagagataaa gatggaaaaa taaagttaaa accaatagca gaagatagaa aagcgtacta
    26101 tgaactattt gaagaagaaa tgaatacaga tagaaattct aatatagttt caaattattt
    26161 atatttttat aatagaataa aagaagataa tatatcaata gatgatttgt ttgatgctat
    26221 acagaaatta actatagtag aaatagagtt aaaaaatggt gaagatgacc ctcaattaat
    26281 ctttgaaagt ctaaattcaa ctggcttaga tttaagtgaa gctgatagag taagaaactt
    26341 tgtgttaatg ggtagaagct ctaagttaca agaggaattt tataataatt attggtataa
    26401 aattgaaaaa aatacaagta gcaacgtaag tagttttata agagactatt tgactataaa
    26461 ggagagaagt ataccgaata aaaataaagt atattttagt tttaaaaggt atgtatcaga
    26521 taatggtata gatatagaaa ttttattaaa agatttactt aaattttcta actattatag
    26581 agagatatct tattcatgta ctgatgataa tgatgtaaac aaagttttga aaaggataaa
    26641 caaacttgaa atatttgtat catatccgtt tatattagaa ttatttgatg attataatga
    26701 aggtataata aataaaaaag tacttg
//
LOCUS       DACULQ010000033        26593 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.033, whole genome shotgun sequence.
ACCESSION   DACULQ010000033 DACULQ010000000
VERSION     DACULQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.033"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(216..1466)
                     /locus_tag="JA154_05750"
     CDS             complement(216..1466)
                     /locus_tag="JA154_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HAT7995364.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1499..2440)
                     /locus_tag="JA154_05755"
     CDS             complement(1499..2440)
                     /locus_tag="JA154_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAT7995365.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2680..3756)
                     /locus_tag="JA154_05760"
     CDS             complement(2680..3756)
                     /locus_tag="JA154_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HAT7995366.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4129..4644)
                     /locus_tag="JA154_05765"
     CDS             complement(4129..4644)
                     /locus_tag="JA154_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HAT7995367.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(5007..6077)
                     /locus_tag="JA154_05770"
     CDS             complement(5007..6077)
                     /locus_tag="JA154_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAT7995368.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGSVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(6084..6575)
                     /gene="leuD"
                     /locus_tag="JA154_05775"
     CDS             complement(6084..6575)
                     /gene="leuD"
                     /locus_tag="JA154_05775"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087472.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAT7995369.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6590..7867)
                     /locus_tag="JA154_05780"
     CDS             complement(6590..7867)
                     /locus_tag="JA154_05780"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087471.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAT7995370.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7824..9566)
                     /locus_tag="JA154_05785"
     CDS             complement(7824..9566)
                     /locus_tag="JA154_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAT7995371.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9690..9936)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10084..10530)
                     /locus_tag="JA154_05790"
     CDS             complement(10084..10530)
                     /locus_tag="JA154_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995372.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTNESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10551..11270)
                     /locus_tag="JA154_05795"
     CDS             complement(10551..11270)
                     /locus_tag="JA154_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAT7995373.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11388..12662)
                     /locus_tag="JA154_05800"
     CDS             complement(11388..12662)
                     /locus_tag="JA154_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAT7995374.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(12862..14229)
                     /locus_tag="JA154_05805"
     CDS             complement(12862..14229)
                     /locus_tag="JA154_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT7995375.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(14479..14916)
                     /locus_tag="JA154_05810"
     CDS             complement(14479..14916)
                     /locus_tag="JA154_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995376.1"
                     /translation="MGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVYVFSVIGSTL
                     VSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGIILFVGIPLPT
                     TGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(14948..15577)
                     /locus_tag="JA154_05815"
     CDS             complement(14948..15577)
                     /locus_tag="JA154_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HAT7995377.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRIIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNKFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(15611..16498)
                     /locus_tag="JA154_05820"
     CDS             complement(15611..16498)
                     /locus_tag="JA154_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HAT7995378.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(16527..17765)
                     /locus_tag="JA154_05825"
     CDS             complement(16527..17765)
                     /locus_tag="JA154_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995379.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(18149..19291)
                     /locus_tag="JA154_05830"
     CDS             complement(18149..19291)
                     /locus_tag="JA154_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAT7995380.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(19432..20298)
                     /locus_tag="JA154_05835"
     CDS             complement(19432..20298)
                     /locus_tag="JA154_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAT7995381.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(20632..22185)
                     /locus_tag="JA154_05840"
     CDS             complement(20632..22185)
                     /locus_tag="JA154_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7995382.1"
                     /translation="MFLANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGI
                     IISRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNT
                     YIGITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAF
                     IGAGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPK
                     KNYIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNT
                     NLKVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDT
                     MEKENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENRE
                     DGLVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDK
                     NFFVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAH
                     SFLVKEGLV"
     gene            complement(22172..23308)
                     /locus_tag="JA154_05845"
     CDS             complement(22172..23308)
                     /locus_tag="JA154_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein"
                     /protein_id="HAT7995383.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(23571..24668)
                     /locus_tag="JA154_05850"
     CDS             complement(23571..24668)
                     /locus_tag="JA154_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAT7995384.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVDVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(25058..26563)
                     /locus_tag="JA154_05855"
     CDS             complement(25058..26563)
                     /locus_tag="JA154_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995385.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
BASE COUNT         8630 a         4833 c         2765 g        10365 t
ORIGIN      
        1 taaaacctta taaatataca aatacaatta tatgaatttt catcctcaat tatagcatag
       61 aatatgacta tctatatcct aaaattatcc atttctaaaa aaaaggtaac tattattaag
      121 aatatcaact tatagaattt atattccaca tacgattttc aatcaaaaaa acaagggcta
      181 aaaagtgaat tgtgcacttt ttagcccttt attaattatt catttatttc atgtaagaat
      241 agaccacatc ttactttagg ttcaaaccat gttgacttag ctggcattat cttatttgca
      301 tctgctactc ttattaaatc ttcaatacta gtaggataca ttgcaaatgc tactttcata
      361 tcttcattaa ctcttttttc taattcttca agacctctaa ttccaccgat aaaatcaatt
      421 cttttatcta ctctaggatt atcaattcct aaaatagcat caataacata gtcttgtaat
      481 atagaaatgt ctaatgagtc aacagggtct tctatttcta ataaattttt atttgctttc
      541 attttatacc acttatcacc taggtacatt ccgaaagttc cttttttgtc aggtttatat
      601 ttttcttttc cttctatttc ttctagttca aaattttctt ttattttatt tataaattct
      661 tcctcgctta aaccatttaa gtctttgata accctattat aatccaaaac cattaaattc
      721 tcatctggtg caatcattgc tatgaagtaa ttaaattctt cttttccagt ataatttgga
      781 tttttagctc tcatttcttt ggctacattt tctgctgaag ctgtcctatg atgaccatct
      841 gcaatgtata gataatctaa tttttcaaat ttatctacta ggttatctat tatatcatca
      901 ttatcaatct tccatactgt atgctctatc ttgtcatctg ttatgaaatc atataaaggt
      961 tcattattat ccataaaatc attgattata tcatctatca ccctttgatg tttgtaagtt
     1021 acaagtatag ttcccgtatt agcatcacag tattttatgt gtttagttct atcaatttcc
     1081 ttttctggtc gagtgtactc atgttttttt attgttccat tcaagctttc atcaactgat
     1141 atacatgcta ctataccaac ttgagctcta ccatccatga tttgtttata tatatatata
     1201 cactctttgt catcttttac tagaatttct tgttttcgaa actcatctaa attttctcgc
     1261 gcttttaaat aaactttttc atcatataaa tctatatttt catctaaatc aatttctgat
     1321 ttatctatat gcaaaaatga atatggattc ccttctgcaa ttcttctagc ttctttacta
     1381 ttcataacat cgtaaggaag tgctgctact ttatctacta tatatttatt tggtctaatc
     1441 gccttaaatg gtttaatcgt tgccattact aatttcctcc ctaattatac aaaatatttt
     1501 ataatgctac ccctattaga ttgttgaagt caagaaagaa atcttgaatc acatttacta
     1561 tttcgtctcc tattctttct tgagcttcta ctgtagatgc cccaatatga ggtgtaggag
     1621 aaactcttgg atgatttact aagtctaaat ttaatgtagg ttcttgttca taaacatcta
     1681 tagcagctgc tgataattta ccttcatcta atgcttttag taaatctttt tcatcaacta
     1741 aaccgcctct tgcacagttt attaaataag cccctttctt catcttattt atttcttttt
     1801 cagttattac tgccccctta ttcttatcaa atggtatgtg taaagataag aaatctgcat
     1861 tttctaaaac ttcttccata tcacagaatt tataattgtt aaagccttgc gcctcaccca
     1921 ttatgtcagt atatataaca ttcataccta ataattctgc tctctttgca acttcttttg
     1981 caattcttcc aaaacctata agtccaagtg tctttccatt aatttctgta cctttatact
     2041 ttttcttctc ccattttcca tctctcatag ttacatttgc agtatttatg aatcttgcaa
     2101 gaacaaataa ttgacctatt gtaagctctg caacagatat actgcttgca tttggagtat
     2161 tcataacttt tatgccatta gcttgagcat atttaacatc tatattatcc agacctactc
     2221 cacctcttac tatcaacttc aactttttac cctctaatgc tttatctatt acatctttag
     2281 taactttagt agctgaacgt actacaagta catcaacatc tttaagttta tctcctaaaa
     2341 catctttttc ataaaactgc tctatcacat taaaatctaa ctctctcaat tttctcgctg
     2401 cttctttctc tattccatca gtaactaata tattatacat aacaaactcc tccaattttt
     2461 attcacttaa tttttccaat ttttattttt taaagtattt tcatcaataa aaaactttac
     2521 ttaatttaac tttctgttta tataattttc aaaatatttt tttattaaag tcatatcaaa
     2581 ttttcctatt gtttgttaaa aatttaatat gtaattttta taaaatatta tttattatat
     2641 attctaaatt tatcttaaaa catacatatt tttacaatat tatttattaa aacctaatat
     2701 atcatcaata ttatctaata attcttgtac atcttcgaca gtatagtcac ccatatgtga
     2761 aattctaaaa gttttttctt ttaaatctcc atagccattt gctatttgga atcctcgttc
     2821 ttgtaatttt gaatttaaat ttgatacact aataccttga gtattttcta taacagtaag
     2881 tgtattggat aagtgatttt cattggtaaa aatttggaag tgttttttag cccagtttct
     2941 aactaagttt gccatgtctt catgtctgtt gaatctgtta tctaggcctt catctaatat
     3001 attatctaat tggaaatcta aagcgaacat gtgagaaaga gatggtgttg aagggtattg
     3061 gtagtttttc ttgattaagt attcatacat tgctaacaaa tcaagatata ctcctctaaa
     3121 aggtacttgc ttagctctat cgattgcttt ttgagaaaat gtgcaaatag acattcctgg
     3181 tggtaatccc agtgcttttt gtactgatgt tatacaaata tctattccaa tcttatcaac
     3241 ctcgactttt attccaccgg ctgaacttac agtatctaca cagtagatta catcttcata
     3301 ctttttaaca acttctccaa tttcttctat tggatttctt attccagttg aagtttcatt
     3361 gtgagttaca gttattaaat cgtattctcc ggtagaaagc accttatcaa ccatttctgg
     3421 cgttgttgcc tctcctaatt caactttaaa tatatccgct gggacattat tagccactgc
     3481 cattttgtac catctatcac caaaggatcc acaggaaaat actgctgcct ttttggatgt
     3541 acaggaacga atggagcctt ccattaaacc agttccggat gacgtggata aaagtatcgt
     3601 gttgtttgta tagaatagtt tttgcatctt ttcacttata ctcttttgga gcatagatgc
     3661 atcttttcct ctatgtccta tcattggtgt agccatcttt tgcaagactt cttctttaac
     3721 atcaatagga ccaggaataa ataatttctt actcattata attcccccct ttttagtttg
     3781 taatatggat agcgttttta ctgtccttat ttttaataac tataatatca agctgaatta
     3841 actatgtcaa cataaaattt attttttata tataatttat tttatatatt ttatgaaaac
     3901 attcattttc taccttttca taagtctgaa ttttctgaat tattctctca aaaaaaatat
     3961 actttttatc tatataaatt aattttttta gtatgtaaat ttgacgctat tacgataaat
     4021 ctttttagta taactaattt atttttatct tgtattttta ttgtattttt gaattttatt
     4081 ttttctaaat ataaaattta tgttttaaaa tgtaaaattt ttatttaatc aaagttcaat
     4141 atattccact ttttctttcg attcttcaaa aaaccattct tcattagaaa aatcaccttc
     4201 tgcaatttgt ccaataggca ttttcatata ctttccagtt gctgtcactg ctatatctcc
     4261 atttggtaga attatttctc ctgtaccttc aaataatttt ctgttttctt tagttattct
     4321 tccaacaact tttatagtag catctgttgg tactggtttc ttatatttca actctaaaga
     4381 tactgttact ccccatattt ggtcatcatt tatacttaca gctcttccaa tagtctcatc
     4441 caatattgca gcagacatac ctccatgaac tctcccagga taactttgat gccaatcctt
     4501 tgtattacaa attgatacaa gctctccatt ttcaagttca taaaaatctg ctttaagtcc
     4561 tagctcattt tgtgttccac aaactaaaca agatttacta ctattttgct tcttcaaaac
     4621 tttatacttt gccaaaaaat ccatctccta accgacatta gtagtcgctt aatattaaat
     4681 aattctaaaa tatattatat aatattaata aaatccctgc aaacttctaa taataaaaat
     4741 aggctacttc aatatgataa gtttaattta aagtattatt ttccaaaaaa taactttatc
     4801 aaaatagaaa ctcaaagtag atataaacct ctttttcgtt attaaaatag taaaaatata
     4861 tttgacttaa tataaaaaag agtgtctcat taactaataa agtttatttt gagacacccc
     4921 tcataaaaca aattatacta caatagattt ttactaattc tacaagttat atatttaaat
     4981 tatattattc aaattaaact ataattctaa atttcattta ctattaattc gcccatttcc
     5041 cttgttccta caacatttcc cacaccatta tagatatcaa tagttctata acctttattg
     5101 agtacacttt gtacagcatc ttcaatacac ttagcttctt cttctaagtt aaaactatgt
     5161 cttaacatca tgcttacaga tagaatagtc gcaattggat ttacaatatc ttttccagct
     5221 atatcaggtg cacttccatg tattggttca tacataccaa aactatcttc tctaagtgat
     5281 gctgatggta acatacctat agaacccgta atcatagatg cttcatcaga tattatatct
     5341 ccaaatatat tattagtcac tattacatca aattggctag ggtcttttac aacttgcata
     5401 gcagcattat caacatacat atgtgacagt tctacatctt caaactcttt agctaagtct
     5461 tccattatac ttctccaaag tcttgaactc tcaagcacat ttgccttatc tacacttata
     5521 agttttttat ttctatttct ggctatttca aaagctctct taccaattct tcttatttca
     5581 ttttcgttat atatttctac atcataagca ttttcgatac catcaatgat ttttcttcca
     5641 cgttctccaa aataaatgcc ccctgttaat tcccttacaa caacaaaatc aatgcctttc
     5701 tcaactatat ccgttctaag tggtgaagct tcttttatac tttcatacat tgtagctggt
     5761 cttaaatttg caaataaacc caatgactct cttaatttta aaagaccaga ctctggtctt
     5821 ttatctccct tacatttatc ccactttggt cctccaacag aaccaagcaa tacagcttcg
     5881 ttttttcgac aagtttcaac agttttatta ggtagaggtt ctccagtttc atcaatcgcg
     5941 catcctccag ctaatacata ttcacaatcg aatctatgat taaatttaca acaaatttta
     6001 tttagaacct ttataccttc atctataatt tccggtccaa caccatcacc ttttatgact
     6061 gctatattac aattcatact ccactacccc ctatttgctt ttattgagtt aattaagcct
     6121 tcatttaata taatattttg cataaactca ggaaatggtt ctgctttata ttcttttcct
     6181 tttgttatat tttttatcac acctgtagaa aaatctactt ctatattatc cccttcatct
     6241 atgttattgg cagcttcttc acattcaagt ataggaagcc ctatatttat agagtttcta
     6301 aaaaatattc tagcaaaagt agaagcaatc acacaggata ctccactttc ttttattaca
     6361 attggtgcat gttctcttga tgaaccacaa ccaaagtttt tagttgcaac tataatatct
     6421 ccttttttta ccttagaaat aaatttatca tcaatatcct ccatacaatg cgttgctagt
     6481 tctttataat cagctatatt taaatatcta gctggtatta ttacatctgt atctatattg
     6541 tcaccgaatt taaaaacact tccattggca atcataaatt ttacctccct taaattattt
     6601 ctgatggttt tgatattttt ccagttatag ctgatgctgc tgctacagct gggcttgcaa
     6661 gatacacctc tgaatcaaca tgccccattc ttcctacgaa attcctattt gttgttgaaa
     6721 tggatttttc tcctgctgca agtataccca tatgtccccc tagacatgga ccacaagttg
     6781 gtgtagaaac tgctgctcct gcctctataa aagtggtaat ataccctttt tctatagctt
     6841 caagatatac tttttgagtt ccaggaaata tcataactct aactccatct gctacttttt
     6901 tgcctttcat tatctcagct actacttcaa ggtctgatat tcttccattt gtacaagagc
     6961 ctattacaac ttggtctatc tttatatcct tatctatctc atctactgtt ctagtatttt
     7021 caggaagatg aggaaaggct actgtttctt ttagttcagc taaatctatt tcgtatactt
     7081 catcatagac tgcatcttca tctgcttcat atatatttac atcttttgtc atagttgaac
     7141 acttatgtga atttatatat tccatagtct tatcgtctac tggaaaaatt ccattttttg
     7201 ctcctgcttc tattgccata ttacaaatag taaatctatc atccatagat aagtattcta
     7261 ctccatctcc acaaaattcc attgacttgt aaagtgctcc atcaacacct atctccccta
     7321 ttatatgaag tattatatct ttaccactta tccatttatt tggtttattc ttcaaaacaa
     7381 attttattgc tccaggaacc ttaaaccaac attttcctgt agccatacct gctcccatat
     7441 cagttgaacc tacaccagta gaaaatgctc ctaatgctcc atatgtgcaa gtatgagagt
     7501 cagcacctat aattacatct ccagcagtta ctataccttt ttcaggaaga agaacatgct
     7561 ctattcccat atctcctaca tcaaaaaaat ttacaactcc ttttgatttt gcgaaacttc
     7621 ttgtaaattt acattgttct gcactcttta tatctttatt aggtgtaaag tggtctaaaa
     7681 ccatcactac tttagtctta tcataaacat cttcaattcc taatttttca aattctctaa
     7741 tggctactgg tgatgttata tcattaccta aaactacatc taaatctgct tcgattaatt
     7801 gccctttctt aacttcattt aatttagcat gtttggctaa tattttctgc gtcatagtca
     7861 tccccatttt ttatttcctc ctcaatataa cttttattta ttgcattcat aagtgcatta
     7921 atagctgctg tatttatgtt ttccgatata cttacaccaa aatagacttc attattgtgc
     7981 tctattccta cataagctac ccctcttgaa tgtgaccctt cctctaatgc atgttcccag
     8041 taatatctaa atttataatc tttcatgcca cattgcttta gtgcattatt aaatgaatct
     8101 acaggtccat ttcctatacc aacaatcctt tgttcatttc ccatataact tattcttgca
     8161 gtcatctcaa tcatattagt atcattagaa tcatcatttt catagtttaa ttcatccatt
     8221 gattttattt tatacttctt aactttataa gggctctcaa cttcaatata ttctttctta
     8281 aacaaattaa atattgcttc actactaagt tcttccccaa ttctatcaga ttccattttg
     8341 actacatttc caaaatcaga atgcatattt ttaggtatca taaatccata atctgtttcc
     8401 attatataaa cagctcctcc ttttccagat tggctattta tccttataat ttctttgtat
     8461 tctcttccaa tatcatgagg gtctattggt aaatatggaa cttcccagta atcattatct
     8521 ctgtttttca ttgctatcat gccttttcta atagcatctt gatgtgaacc tgaaaaagca
     8581 caatgtacaa gattacctgc atatggatgt ctatcatgta ccataagttt tgtacagtct
     8641 tcatatatac ctattatctt atctatattg ctaaaatcta gctctgggtc tattccttgt
     8701 gtataaagat taagtcccac atttactata tccatattcc cagttctttc accatttcca
     8761 aatagtgttc cttcaagtct atcggcacca gccaataatc caagttcact tgcagccgta
     8821 caagttcctc tatcattgtg agtatgcaaa cttagtataa tagaatccct acaacttata
     8881 tttttacaaa accattctat ttggtctgca tatacgtttg gtgttgccat ttcaactgtt
     8941 gatggaagat ttattattac ttttttttga ggagttggtt tccaaacatc tatcactgcc
     9001 tcacatattt caagtgcata atccatctct gttcctgtaa aactctctgg tgaatattca
     9061 aaagtaaatt ctgtttctgg atattttttt acttcttcat taaataattt tgctccttca
     9121 actgctatat ctattatttc ttgtttactc atattaaaaa ccacatctct ttgaagaaca
     9181 gatgtagagt tatataaatg tataattgct tttttacatc cccttattga ttcaaatgtt
     9241 ttttctataa gatgagccct agattgtgta agtacttgta tagttacatc atctggaatc
     9301 atattctcat ctattaattt tcttaaaaat gtatactctg tatttgatgc tgatggaaat
     9361 cctacctcta tttctttaaa tcctgtatca ataagcattt tgaacattct gactttttca
     9421 tttacactca taggtgttgg cagtgactga tttccgtctc ttaaatcgac actacaccaa
     9481 attggagcct taaatatttc attatctggc catttacgat ttactatttg cattttatca
     9541 tattttttat actttccaca cttcatgtta tttccccctt tttctgtttt ttgtattcag
     9601 tatacctcat atcgacgcaa tcctgcacaa ttcataagga taaatgtcaa ttttatcatt
     9661 agaataaaaa aaacctcatc tctaataaaa ttagagacga ggttttgctc gcggtaccac
     9721 tctatttagc tttctaaaaa gctcccttag taatagccaa tacaataatt ataatgacta
     9781 tatttcctat aacgtggaat tactccgatt attctaagat ttaaatctta gaaaacagct
     9841 cctagacaag ttcagaacta tcaactacta aatcacacca tcctttagct ctctgaaagt
     9901 ttcattgttc ttactacttc tattcatcac ttttttaatc atttaaattt tctaaatatt
     9961 ttgatttaat agttattata tacacattaa aatgtattgt caacacaaaa aataattatc
    10021 tcaatttttt atttttgata agcaattcta taaaacatgc taactattga aaacagtatg
    10081 atattatgcc ttaaactttt tcatcttttc atctatgata ttcaataatt ttttttcatc
    10141 ttccaaactc aatgccctta atctaatttt ataattagat tcgttagtgt atatatttaa
    10201 tatatttttt actacttcta ttttttctat tatcttataa ttaaactttt gactttgttt
    10261 agattttata ctgaatatac attgattatt tattaatatt gaatgattat cgagagtaat
    10321 atatactttt ccctcatatt tatttgaaaa atcacgacct tgtttaaata gaaaaactgc
    10381 tgttattata cataaaatat ataatattgt cgttgaatat ataaaaattg tagctaaaat
    10441 tataagtgct aaaaccatat atatagtcat tcctttttta aaataaccat tgtcataata
    10501 attcatatag ataaacttat ttttactcaa attttttcac tcccaagatt ctaaaaattt
    10561 atatcaaaaa attctataat ttcatcttca tgataattta tgactaattc atctggaaaa
    10621 tctactgctt ctaaaacttt ttctgcattt tcaaattctc ctatagaata acatatatga
    10681 gagtcagtac caagaattac tcttgcacca tatgttttac acaatgtcaa catatgactt
    10741 acattttccc aagtacccgt tctatgagag tttgaactta atgatgaatt atttatttct
    10801 aaaaggatat ttttatcttt agcttttttt actataggtt cataatctaa aggataccta
    10861 ctgtcatctg gatgtccaat tatttttacc ttaggtttat ccataacttt taaaatagca
    10921 tttgtatttt cttcttttgt accagaagct atacaaggtc tatgcaaact tgcgattata
    10981 taatccaaat gttgcaacat atcttcttga acatctaaat taccattata atccattata
    11041 tttccttcta ttcctctaag aatctttacg ccttgcactt cacgtggaag caaatgcaaa
    11101 ttataaaaat agaatggatg tggtcctcct ggcatattag gtccatggtc tgatattcct
    11161 aagtatttta tattcttctt actcgcctct tcgacatttt ccttgaaagt actatgtgca
    11221 tgtccactta ttagagtgtg acaatgtaca tccataatag actttttcat taaatttcct
    11281 cctaatattt tacataaatt cgtgaaaata gctaaagggc tatttcaaaa ctaaagctgt
    11341 cataatagac agtgtaacgt tttaaaacag ccccattttc aattatttta cacttctaaa
    11401 tctttaaatt tatctttaaa taattctcca atacttccta aagattcctc tacttctagg
    11461 tattctgtta tatcctcttc tggctctcta gttgcttctt ttattgaaac caacattctc
    11521 ttagtctttg gtttaaaatc aagaatctta actttaacct catctccaat attaacaaca
    11581 ttagtaactt tagccactct agtatcactc aattcagata ttggtaaata cgcatctaca
    11641 ccttctttaa tttgaacaaa agcccctttt tctattattc taagaacttt agcattgaca
    11701 acatcatcaa tttgtacttc ttttgctatt aaatcccaag ggtcattatt tatgtctttt
    11761 aatgccaagg caattttttt agtctcttta tttacatcca acacataaac ttctacttct
    11821 tgtccctctg aaacaacatc ttcaacactt tcaactcttg tccaagctaa attatttata
    11881 tgtgctaatc cttcaatccc accaatatcc acaaatgcac cataaggcat tatctttgtc
    11941 accttaccat ctcttttttg tccaacttca agagaattaa ataagtcttc tttagctgct
    12001 ttgatacgag ctttttcttc ttctctttct tgcttaattc ttgctctttc agcttctttt
    12061 tctgccttaa tttgagctct tctttcttct ctctctttat ttatacgctc ttgtaataca
    12121 tctctatgag atgctacaag tctatttttt ctaacatcca attcttttat ataaacttct
    12181 aagttaagac caacaaattt agatgtatct gtaataaatt ttgtatcaag ttgagaaatt
    12241 ggtataaaaa atgtatatgt attgtaatta gcaaatacac ctttatctat attcttttca
    12301 acatttacag ttattattct atgttcatca taagcctttt gtaactcggc aaaatcattt
    12361 cttttgtcaa gttgtaattt agataaagtt attgtaccat ctttttgaga tacttttgtt
    12421 attacagcag ttatttcatc acctatgtgg tatatatctt ctatatattg atttttttct
    12481 atgttcaatt cacttatagg tattattcca tcaaacccat agtctaatcc taattgtact
    12541 tcatcatttc tcactgcagt tatttttcca gttgtcaatt ctccaacttt cactttgctg
    12601 aatacttgtt cttgctggtc aagtaattct tgcattgtta aatcattttc cattataaac
    12661 atcctttcct cttattgatt atcatatctg taaatattat accattaata ttgcaaatac
    12721 ttacattttt cttactataa tttttaaaat aaattcaact tcatatattt atacattata
    12781 gaaaatatct tattaaatta aaatacaaat tctaattttt atctaataaa atattttcat
    12841 aaaaaattta tattgtatat cttattcttt tattgcttta ggttttgata gaaaacattt
    12901 ataaaatata aagaaagaaa ttgctgttat agtagcagct gttatatctg caactggctc
    12961 tgctaaaatt actgcaaaaa gtttttcatt aaagaatgtt ggaagtataa atattaaagg
    13021 tattaaaagt acaatctttc taagtagagc taaaaccaaa gatattgttg cttttccaag
    13081 tgctaaaaat gtctgttgac aagctatttg aatcccaaac ataaaagcac cagcaaaata
    13141 tatttttata ctccaactag tcatactaag taactgcgga ttcttattaa atatactaac
    13201 aaaaaatgca ggaaatatca ttaataaaag ccataaaata gtagaatatg taaaacatga
    13261 caacaaacac aacttaaatg tcttttttac tctatcaagt tgacctgaac cataattata
    13321 acttattatt ggttgtgctc cttgtgtaac ccccatatta ggaagcataa gaatttgcat
    13381 tatactgctc attatagcca ttgaacctac tgctaaatct cctccataca tttgtaattt
    13441 actatttaag gctataagta ctaaactttc tgtagattgc attataaatg gagagattcc
    13501 caaaccaagc actggtaaga tgatacttgc ttttggtatc atatacttct tttttatttt
    13561 aagtatactt ttctttccaa agagaaacat aagtacccaa atagcagaaa cgccttgtgc
    13621 aattatagta gctaatgcag ctccttttac acccatatta aagccaaata taaaaatcgg
    13681 gtcaagtatt atgtttatta aagcacctat agcaactgtc atcattccag ttgtagcaaa
    13741 cccttgggta tttataaaag tattcatacc cattgaaatc tgaacaaaca ctgtacctat
    13801 taaataaata tttaaatagt ccattgaata tccaattgta gattcacttg ctccaaacat
    13861 ccaaagtaaa ggctcttttc caatcttaaa acctatagtc aaaagtattg ctaaaatcac
    13921 cagtacagaa aaactgttac tcattatttt ctctgcctca tcattgtttt gctcacccat
    13981 ttttatagca gctaatggac ctcctcccat accgattaaa taactacatg cagttattat
    14041 aattattatt ggaaatgcca ctccaacacc agccatagca acttctccat tttccattct
    14101 accaataaaa atcctatcga ctatgttgta taaaacattt acaatttgtg cgataatagc
    14161 aggtgtagca agtttaaata ataatttacc cacactttca ctacctaagt tagtgtttga
    14221 attttccatt ttaaaacctc ctcaatccaa taattaaaat gtatcactaa aattttattc
    14281 tatataaaaa tttcatttta ttagcagaca aatttagagt actttcccta caagtaatac
    14341 tatactatat ttcttttgcc atagtaaaca ttttagaact aactgaagtt aaatacttta
    14401 tctcattttt tatttattat taaaaatttt tcacacatac tcataaaaaa actatccaag
    14461 tatattactt agatagtttt atattatatg taaagaaaga gctgaaacta taacccctga
    14521 taacaaattg ccaagaaata ctcccataaa agcatctttt attctcattt ttaatatgga
    14581 agctatagca gatgcagtcc acgttcctgt agttggcagt ggtataccta caaatagtat
    14641 tataccaatt atcgaaacac tttttaattt cttcattctt gagtttattt ttctatcaat
    14701 tacatcagca atccctttaa atagtttctt ccctcttaaa aattgtaata catgtctgaa
    14761 tgtcaatatc aagggaatcg ataccaatgt agaacctatt acactaaata cataaaccca
    14821 tataggattt aagcccattg caatacctat aggaatggct cccctcaatt ctgttacagg
    14881 aaccatagat aaaattaata tgtgtaaata tcccaaacta ttccctccta ttctaagtga
    14941 cattaagtta tgaatttatt tgctctataa cctcaccatc ctttttacta actaaaaatg
    15001 catttttagt caatgaaagc attggtaaat cactcttaga gtctgaatat gcatatgaat
    15061 tttcataatc aatctctatc ccaagtttac ctaatacagc ctttattctt tttacttttt
    15121 caattccctt acagttttct ccaatgaatt tatttttata tcttgaattt tcataaaaaa
    15181 actccgtgcc tataacctcg tctgcaagac cataatactt aaaatatttc atatatgcat
    15241 atggagatgc agttatcata attataacat tatcttcttc tttatttttg taaataacct
    15301 ttttcataga ttcgttaatt tttttcatta tgcaatcatc aaaaaacttt tttaaatctt
    15361 cttcactaaa atctactatt ggataaaaga ttgcctcttt tgccttcttc aatggtatta
    15421 ttctaattaa atacataaat agaataaaaa taatgtatgg taactttata atagttttaa
    15481 aggttttatt ttttaaagag taaataaaaa aatctatcat gctatcagta tttataacag
    15541 tataatctac atcaagtaat aatacacttt ttttcataag tatttcccca tcctatcact
    15601 aaaaatatca ctaaaaaatc aatattatta aacatgtaat tatccataag attacgttta
    15661 tttttaaagc attatctttt aagacaatat cagtaggatt attttcattt gtagtgttaa
    15721 ttatataatt atatctaagt acaccataaa ctacaaatat tgttgtaaat atcatatgtg
    15781 gcttttctgg attacttgta gaatacaatg cataacatac aattgtagaa cttaatataa
    15841 ctatcatcat ttgatttaga ttttctatgt cataatcttc caatattttt ctatgttcaa
    15901 ctgcatcatc tcttagagtt tctatttctt ttttacgttt tccaagagct aagtacagag
    15961 ataagaaaaa tgtacataaa attatccaat ttgataaact aacacttatg gctacacatc
    16021 ctgcataaac cctaagtata aaaccaagag atattgaaaa cacatctaat agtattatat
    16081 tctttagttt aagagaatat aatatattgt ttatcaaata gagactgaga attatagaaa
    16141 gattaaaact aacaactgtt gataaacaga ctgaaattaa agctaatact acaaatagta
    16201 tattagcact ttttttgctt attcttccac ttgccactgg tctattacat tttttaggat
    16261 gttttttatc tttatccatg tctatagcat cattcagtgc atatactgat gatgacatta
    16321 gacaaaaaca cacaaacgca ataaacgtat ttaaaaataa atttatattt agtatgttgt
    16381 ttgaaaaaat tatagctgaa aatacaaaga aattctttat atattgcttt attctcatta
    16441 actttatata atctgaaatt ttagtaagaa gccttatact tgtattggtc tgtttcataa
    16501 acttactacc ctttcaatat tattttttaa acaggatatg attttatccc tatgtttaaa
    16561 atttctataa atccattgac attactgatt actatagtaa ccaataacag cgatagaaat
    16621 atattataca tattcttttc attttttcta agcgctttcg taaagtaagc caataaaatt
    16681 ataactgcaa aactaaaatt tatagccata atacttgaat tgactagatt atatcctact
    16741 ataccatgta aagttagatt gaatataatt attcccatac atagcttagg aactcttttt
    16801 tctgaatttt caataactga taaaacacaa aaacataaag ctaataatgt aagaattact
    16861 ggtattgtag caacttctcc attttgcaaa aataaccctt ttttaatact tgctccagga
    16921 aaataaaaaa ccgaatacaa taatctctct ataatcattg caatcttttc caaaattggt
    16981 tctccactta taaatttcgt atcatactgt attacatttc caaggttcat aaaactctca
    17041 taagactttg ttgaaactac acaaatatat gccattagga taaatgtaat taaatgtttt
    17101 aacatttttc ctatttttaa tttaaactca aatatcatat taaaaaaata caaatatata
    17161 ttactaatag ttattccaaa tagaggaata gctgctaatt ctactaaata agaatttttt
    17221 cctttcatct tatttgcaat aactatgaaa aatattaatg aaaattggct atatataaat
    17281 ctctctacat ttaaagtcat aaataactgt ggaaaagaaa atatcattat catagtaagt
    17341 aaaattaaag ttaatctctg catttttatc tttttaatat gattaaagac taaagttatt
    17401 gacatagcac tagttattat ttgtagacaa attagtccaa acccataggc actattaaaa
    17461 tctgttgtcc caggatttgc tattgaagta caaaataggt atattggata tgcaaaaatt
    17521 gatataattg ctgaaaacaa tatgtgctta ctattgtctt gacttatagc aaatacattt
    17581 aaattgaata aaactcctgt atctgtatct aacaatacat cataagtatt gtttaaatca
    17641 taaaatatag tagtcttaga aaaaaatatc ttagttatta ttaagtaagt tattacaact
    17701 aatataaaca aaaacatctc gtttttatat tttaatattt tactcatcaa tatatcacca
    17761 tccattcatt atataggatt tgctgacacc tacaatttat tccttatatt taatagccat
    17821 cttcataatg ttcttacttg ttttattgta attgaataac tctttattta caaccacaat
    17881 atttacttta ttgatgttat aatcagtcaa gtattgttta tataacatag attagtgttg
    17941 tataaaatga gaatttgtaa gcaatattaa tgtaaaatat ttgaaaaact ttatataaaa
    18001 atatacaatg ttgcttatgg aattaaacta taacaaatta aagcataatt taaggagaca
    18061 aaattataat ctgtctcctt aaatattaaa actatatttt aaaacattac taaatactat
    18121 acaaatttca aatatagtat aaaattaatt agactttaac actattagct ccatcttctt
    18181 cccatttttt aatttctttt aatctatcta ttataggtaa atgctgagta cattcaactt
    18241 cacattttcc acaagatata cattctttag cttttgcctt actattcatt gtgtaattcc
    18301 aatgtcctaa gccataaact aactttgtca tttcttcatc actcttttta aacatctgct
    18361 tttcattata aaaaagcata taggctgggg tattaatacc ttttggacat actttacaat
    18421 aaccacaacc tgtacatatt tcgttcatat tcttatttaa tctagtctca atatcatgta
    18481 tatcctcatc tgaataaatt ctattttcgt ctgcaatttt acatgcatca tctatatctt
    18541 gttttttatt aaaccctatc aaagatatac ttatttgtga aattcctatg ttgaatctta
    18601 aagctgattc tatagtactt tcgccatctt ttgatagaaa agaaagttct ttattatact
    18661 ttggaatagt tccaccactt aatgggttca ttgccacaac accatatcca ttatcaacag
    18721 catatttggc accttttagc ctatatggaa agtttaatat atttattccc atagttacac
    18781 cttcaaactt attttcactt agaatatcta taacctcatc tcctggttga tgagatgaaa
    18841 atactatatg gtctatcaat ccttcttctt tacatttcaa taatccttca tattgacctc
    18901 caggtctcat tgctagttcg taatgttcca tctttctaat attccaaaca tgataaaaat
    18961 ttattttaga tacgcctaat cgttctattg atttttttac agcctctata gctttttctg
    19021 atgtatcaca tgcttttggc ttttctttag ttgatacata aaaatcggtc ttaccttcct
    19081 ttatcatttg tctaaaggct agtccaaata tatcttcact tctgtcatca caataccctg
    19141 gtgctgtatc aaagtaatta ataccttttt catacgcata tttgactaaa tcagcatttt
    19201 cttgattact tttttctaaa tcaaacctta atcctccata gccaactact gaaaccattt
    19261 tccctgtctt tccatattca acgtatctca ttgtaaattc ctccttagat atattaagac
    19321 ttaatttctt tatagaaaat attataccat aaataatttt tataaattat ttatggtata
    19381 atattttttt tataaattat atttttttat aacttgtatt ttataactaa gttaaaaact
    19441 ataaaaaggt tcaatatgga ctattacttg agcattttca ttaatttctt cttgtatttg
    19501 tttctcaatt ttatgtgtta aatcatgaga ttgctctacg cttataaatg ggtctaccat
    19561 tacatgcatg tcaatataaa tagaactttt gctacctcta cttctgatat tgtgaacatc
    19621 ccttacctca ttaaattcaa acacaatttc ttttatataa tcctcatcta cagccactct
    19681 atcgaccaaa attccaatag atggcctaaa tattccataa gccgaataaa ttatgaatgc
    19741 agaaataata ataggaacta tagattctat tattactgga aatcctaact taacaccaac
    19801 taaagtaacc aatacaccta gagacacaaa tatatcactt cttgtatgca atgaatccga
    19861 aatcaaaaca taactattta gtttagtccc aattctatat tcatacatgc atacaaaaat
    19921 atttataaaa agtgtaataa ttaaagcaat taaactttct atagttatag caggtacaac
    19981 tggattagct attcttaaca ctgcactaag aattatctta attgctataa caaacaacat
    20041 accacttata aataaactgg ttataatttc aaacttttta tgtccataag gatgattttt
    20101 atcttttggt ttagatgcaa agaaaatacc tataagcccc actatattag atgctccatc
    20161 tgacaaagaa tgaaatccgt ctgctgtcat actatagctt tttatttgat taccaataat
    20221 gatttttaat aatgctactg caaaattagc aaataaaatt atccacaata cttgcttcac
    20281 tttcttatag ttatccataa caaattcccc ctcccttcga ttcaataaag aaagctatct
    20341 atcaatatca atcaaccaga caataaaatc gtatataagc acatttacta tatttatatt
    20401 caaaattggt tgtacttgtt cattctattt gaaacttaat tataaataaa aatcgtaggt
    20461 atggttttgg tatacataaa aataagagac tgtctcaaaa taaagataat cttctttttt
    20521 attgttgaaa tgataaaata tatttgattt catataaaaa agagtgcctc atgaactaaa
    20581 aagttcattt tgagacactc ccttaaattt ttgatataaa ctattaattt tttatactaa
    20641 gccttcttta actaagaatg aatgagcaac ttcttctggt gatttaccaa gttcatccac
    20701 ttgatagtta agttctctca tagtttcttc atttattttt cctgaaagca tattcataac
    20761 tttttctaac tcaggatatt tcttcaaagt atcttctctt actaatggtg ctgcataata
    20821 tggtacaaag aaatttttat cgtcctctaa tatcttaaga ttaaactttt taataagacc
    20881 atcagttaaa aatgcatcta taacctgaga ttcattatta tctaaagctg tatatcttaa
    20941 acttccattc attgctttta catctttgaa ttttaaacca taatctctag aaagccctac
    21001 aagaccatct tctctatttt caaactccaa agttggtgag aatgtaaatt ctttaccata
    21061 tttagtcaaa tcagaaatag tatttattcc atacttttca gcagtttcag gcatcatagc
    21121 taatgtgtaa gtattattaa atccaagagg gtcaagtaaa gttaactgat tttctttttc
    21181 catagtatct tttacaacat tatatgcttc atcagcatct ttaatcggag catgtttcat
    21241 tacatttacc aaaagtgtac ctgtatagtc aacatacatg tctaactcac cagatttaat
    21301 tgcattaaac gcaacagatg aaccacctag atttaaattc ttcttaactt taagattcgt
    21361 atttttttct actaattcag cataaacatt gccaagtata agttgttctg tataatcttt
    21421 agaaccaata gttattgtat cttttttact tgaaaaacta gaactaaaaa caacgaacaa
    21481 cattgaaaca acaagcacta cagatattac tttaagtgct atataattct tcttaggtgc
    21541 ttttggattt aatccttttg gagtaactgc aacttcaatt ttactaaata aataatcaac
    21601 taacagtgca attatacatg atgggattgc ccctgctaaa atcatattat tatttacagt
    21661 ttgaacccct gaaaatacta aatatcccaa accaccagca cctataaatg ctgcaagagt
    21721 catcaaacca acagctgtaa ctgcagatat tctaactcca cccattatta taggaagcgc
    21781 taaaggaaat tgtattttaa ataatgtctg atttcttgta agaccaatac ctttagctga
    21841 ttctaatact actgggtcaa tacttgttat acctatatac gtatttttta caattggtag
    21901 taatgaataa accactacca taaatatggc tggtttactt cctataccaa gtataggtac
    21961 aagaagaccc aaaagtgcca tagacggaac tgcttggact aagtttacaa atccaagtac
    22021 aaactttcta agagatgaga ttcttgaaat aattattcca agaggaacac caactattat
    22081 agctattaaa atagatgtaa cagttagact catatgttgt attaaaagct ctataatctt
    22141 atctttttgt aatagtataa agttaaaaaa attagccaag aacactcacc tccatttcta
    22201 agaattgttc acttaaaaca gaaagtaaac tacttctagt aataagccct accagtttat
    22261 tttcatcaga aataaccgga atatatccaa ccgaattacg attcattaca tttagtattt
    22321 ctactaaatt atcaccttca tttacatgca aaggctcact tgacataata tcagcaagta
    22381 atattgactt atcatcaata tctttcatat cttttacagt aacaatacct tttaatgttt
    22441 ttgctctatc tataattagt aagctatcaa ccttactcgt tctcataatt tctattcctt
    22501 gagttacagt tctagtagaa ttaacagcaa taggattttt tatcataata tctcttgctt
    22561 ttatatattc tggattatcc caaactctat cttctccgat aaaatctttt acaaagtcat
    22621 ttgcaggctt tctaagtata ttttcgggag tatcatattg tgctattctt ccatccttca
    22681 taatacaaat tttatcagct atctttaaag cctcatccat atcatgagtt acaaatatta
    22741 tagttttttt caattcatct tgaagagaaa ataattgttc ttgtaaagat gttctcgtta
    22801 ttgggtctaa agcactaaaa ggctcatcca tcaatattat ttcagcatca gtagcaatgg
    22861 ctcttgcaac accgattctt tgttgctgtc caccactaag ttctgaagga tacttattca
    22921 aaaattcatc actatcaagt cctaccatct caagtagttg caacgttctt ttttcaattt
    22981 cttcaacgga tttttccttt tttaggcgtg gaattagctc tatattttct tttattgtaa
    23041 gatgagggaa taggcctgtg ttctgtataa catatcctat ttttcttcta agctctattt
    23101 cattttcctg agaaattggt ttgccattaa tatatatctc accagatgta ggttttatta
    23161 atttgtttat aagcttcaaa gtagtagtct ttccacaacc acttgaacca atcaaaacta
    23221 ccaatgtacc tgtttctaca actagagaaa tatcatctaa tataacatta tttcctattt
    23281 tttttgttac atttcttatc tctatcataa tttatcatcc cctttttcga acaagcaacc
    23341 ttcttcaata ttataagttg tttttataat tatatcatac tattttattt atgtaaacaa
    23401 agtacgattt ttaggtcact atttgtctga atattgctac aaatataaag tatttatata
    23461 tagtatataa tacataatgt caaaaaacaa aaaatataaa aatattcata aaataacact
    23521 agcctcaatt atatagtatt aaagctagtg ttatttcatt taaacaaaac tcacatttgg
    23581 atactatcca tgtattcaaa taatgtaata tctttataag tttctttttt atcacttagg
    23641 tttgatattg taactcccaa tagcctaatt tgttttccat taacatcaac atttctcaac
    23701 aaattatcta caccacttct tatatcatta tattcgtcaa tatagtgttc taaactaagg
    23761 cttctagtta tctttgtaaa gtcctcatat ttaattttta atgttaatgt ttttccaaac
    23821 ttctcagagt tttttaatct atgacatact tcttcacaaa gttcatcaag aatgttgcgt
    23881 gtttcttctt catcaatatc caaattatga cttaatgtag tctcagcccc aacagatttt
    23941 ctaaccctat ttggttccac tggtctatcg tctattcctc tagcaaattt atataattca
    24001 taacctcttt ttttgaatat attttcaagt tcaaataaat ttaaacacct taaatcatat
    24061 ccagttttta tacctaaatt tttaagagta tttgaagtaa cttttccaac accaaaaaat
    24121 ttattcacag gtaatttatc taaaaagtcc tgggcatttt cttcggttat tacagttaac
    24181 ccatttggct ttcttaagtc tgatgcaatc ttagcaagaa acttattgta tgacacacca
    24241 gctgaagaag taagaccaac ttctctaaat atatcttttt ttatttgctt tgctatctca
    24301 attgatgaat caatattttt tttatttttt gttacatcta agtaagcttc atctaaagat
    24361 aatggttcaa ctaaatccgt gtatctataa aatatatctc ttatttgctc tgacacactc
    24421 ttataaacat caaatcgagg tcttacaaaa actgcatatg gacacctatt gtaagctaca
    24481 gctgatggca tagctgaatg gataccaaat tttctagctt cataagaaca cgttgcaacc
    24541 acacctcggc tttgtggatt tccaccgaca ataacaggtc ttccttttaa ttttttatta
    24601 tctctttgtt ctatagatgc ataaaaagcg tccatatcta tatgaattat ttttctataa
    24661 tgtttcatta ttatcacctt ttaaagtata aatagattaa ttttatttat attacttttt
    24721 aattatacca aacttatgtt tctttttggt attttttcat tattttcttt atatattcat
    24781 attttccata aataaaacca tctataatcc attaacttca atataattta taaaaaatcc
    24841 ttaaaaacac ttgcaaaaac tatataaaag ctttacaata ggtattccaa ggaactaaat
    24901 ttaaaattcc ttatggataa cagtatatat atttaattta tgggatgttt taaaaacaaa
    24961 agactgtctt atgcaagaca gcctactgat tttattataa ttgtaatgga ctgctcaaac
    25021 tattacttta aaacagccca tattttttta tataatttta actatagtaa agattttgtg
    25081 aaggatattc tgggtcacaa gttacatcta taccaagctt tctaaatgtt tgttcatcat
    25141 ctctatttaa tatagtagta gaatgagctt gacacccttt taataatgct aacttttcca
    25201 tagcaaattg tgcagttgga ttagttacag cacaaatact aagtgcagtt aacacctctt
    25261 ggcaactaag tgaaacattt ctactagcta aagtctttgt ttttaagttt atgataggtt
    25321 ctaatattac aggtgatatt aagtgcatat catcagatat attggctaaa tgtttaatgg
    25381 catttaatac tactgctgca gttccattca ttaaatcaga acccttacca gttaatattg
    25441 taccatcttc taattctaat gcaactacag gacaagtatc attcttgtca gcactttctt
    25501 tcaatttatt actgtagttt ctagcaggta atacaacatt tctatcttct ggctttaagt
    25561 taagctcttc cataatcatt ttcattctat cataagcacc tttatcaact tgtccctttt
    25621 tatactcaca ggcagtttta aagtatcttc ttattatttc ttctatagaa gcttttctta
    25681 ttacttcatc atctattatt ccaaagccaa ctctattaac acccatatca gttggagatt
    25741 ggaaaattga ttcttttcca gttatctttt ctattatttt tttaagcact ggaaataatt
    25801 ccaaatctct attgtagttt actgacattt caccatacgt ttcaaggtgg aatgaatcta
    25861 tcatattcac atcttttaaa tcaactgtag ctgcttcata agctatattc aaaggatgtt
    25921 ttacaggtac attccaaact gggaatgtct caaacttaga atatccaact acattacctc
    25981 ttttattttc atgatacaac tgacttagac aagtaccaag ttttccacta ttaggtcctg
    26041 gtgctgtgac tactactatt ggctttgtag tttctatata ttcatttgct ccatatccct
    26101 catcactaac tatagtctca acatctgacg gatatccctt tgttggataa tgcttataaa
    26161 ctttaattcc tctacgttct aatttattta taaatacagt tgtagcaggt tgtccttcaa
    26221 atctagtaat tactacacta tttacatcta gttcataacc tcttaaatca tctattaatc
    26281 ttaaaacttc catatcataa gtgataccaa agtctcctct tattttgttt ctttctatat
    26341 ctcctgcata gacacaaatt acaacttcaa ctttttcttt catatgttgt aatactttta
    26401 tctttgcatt ttcatcaaat cctggaagta ctctttttgc atgaaggtct cccatcaatt
    26461 ttcctccaaa ctctaaataa agtttgtcaa aattatttac tctttctaag atatatttag
    26521 actgctcttc taaatatttt ttgtggtcaa atcctacttt catcttaaca cctcactaaa
    26581 ttaaatttct tat
//
LOCUS       DACULQ010000034        26332 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.034, whole genome shotgun sequence.
ACCESSION   DACULQ010000034 DACULQ010000000
VERSION     DACULQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26332
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.034"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(141..1220)
                     /locus_tag="JA154_05860"
     CDS             complement(141..1220)
                     /locus_tag="JA154_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT7995386.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      1508..1623
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1798..3168
                     /locus_tag="JA154_05865"
     CDS             1798..3168
                     /locus_tag="JA154_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7995387.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFVGLILTVFFMRK
                     TDKSIS"
     gene            complement(3434..4018)
                     /locus_tag="JA154_05870"
     CDS             complement(3434..4018)
                     /locus_tag="JA154_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAT7995388.1"
                     /translation="MSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLLNRRNFARTS
                     QTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLESRQELCSIFL
                     LVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIRKKLQMESSE
                     KVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(4035..6398)
                     /locus_tag="JA154_05875"
     CDS             complement(4035..6398)
                     /locus_tag="JA154_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAT7995389.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            6578..7090
                     /locus_tag="JA154_05880"
     CDS             6578..7090
                     /locus_tag="JA154_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HAT7995390.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            7078..7605
                     /locus_tag="JA154_05885"
     CDS             7078..7605
                     /locus_tag="JA154_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HAT7995391.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(7676..8926)
                     /locus_tag="JA154_05890"
     CDS             complement(7676..8926)
                     /locus_tag="JA154_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAT7995392.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(8945..9529)
                     /locus_tag="JA154_05895"
     CDS             complement(8945..9529)
                     /locus_tag="JA154_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HAT7995393.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(9679..10965)
                     /gene="tig"
                     /locus_tag="JA154_05900"
     CDS             complement(9679..10965)
                     /gene="tig"
                     /locus_tag="JA154_05900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089822.1"
                     /note="Tig; RopA; peptidyl-prolyl cis/trans isomerase;
                     promotes folding of newly synthesized proteins; binds
                     ribosomal 50S subunit; forms a homodimer; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAT7995394.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(11159..11629)
                     /locus_tag="JA154_05905"
     CDS             complement(11159..11629)
                     /locus_tag="JA154_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAT7995395.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTHMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(11629..12978)
                     /locus_tag="JA154_05910"
     CDS             complement(11629..12978)
                     /locus_tag="JA154_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAT7995396.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKIIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNIPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(13138..15162)
                     /gene="ligA"
                     /locus_tag="JA154_05915"
     CDS             complement(13138..15162)
                     /gene="ligA"
                     /locus_tag="JA154_05915"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089825.2"
                     /note="this protein catalyzes the formation of
                     phosphodiester linkages between 5'-phosphoryl and
                     3'-hydroxyl groups in double-stranded DNA using NAD as a
                     coenzyme and as the energy source for the reaction;
                     essential for DNA replication and repair of damaged DNA;
                     similar to ligase LigB; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAT7995397.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(15348..16286)
                     /locus_tag="JA154_05920"
     CDS             complement(15348..16286)
                     /locus_tag="JA154_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAT7995398.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(16388..16810)
                     /locus_tag="JA154_05925"
     CDS             complement(16388..16810)
                     /locus_tag="JA154_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HAT7995399.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            16998..18281
                     /locus_tag="JA154_05930"
     CDS             16998..18281
                     /locus_tag="JA154_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7995400.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(18359..18874)
                     /locus_tag="JA154_05935"
     CDS             complement(18359..18874)
                     /locus_tag="JA154_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter HydN"
                     /protein_id="HAT7995401.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNLDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(18874..20259)
                     /locus_tag="JA154_05940"
     CDS             complement(18874..20259)
                     /locus_tag="JA154_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HAT7995402.1"
                     /translation="MTFMIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCV
                     MCGQCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSD
                     PNVFTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIME
                     EGSELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYG
                     AEINNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGID
                     FKNLREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGEN
                     GFRKSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQP
                     KILLEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLH
                     TTYCIGKEVAK"
     gene            complement(20275..20826)
                     /locus_tag="JA154_05945"
     CDS             complement(20275..20826)
                     /locus_tag="JA154_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT7995403.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRQCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(20884..21057)
                     /locus_tag="JA154_05950"
     CDS             complement(20884..21057)
                     /locus_tag="JA154_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995404.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            complement(21068..21826)
                     /locus_tag="JA154_05955"
     CDS             complement(21068..21826)
                     /locus_tag="JA154_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase FdhD"
                     /protein_id="HAT7995405.1"
                     /translation="MDYKYLNSFNVEVSKINEHEVSIDNEEIIAEYPLSFILNDKYIN
                     TFLCTPYNLEELIVGFLKTKGYIENKKSLLKFEINESERFAKVTIQEKQKNQDEQIIF
                     LNSLDYITCAPVKSNIKINYDTIYSIMDRNLNSSELFKNTGGVHSVSVFNFDKKIVTC
                     EDVARHNAMDKAIGFCVLNDITLKDKIVAVSGRISLEMILKAAMTQIPIVVSKSAPTN
                     LSIELSKKLNITLIGFVRGRRMNIYANPQRVIKK"
     gene            complement(21950..24094)
                     /locus_tag="JA154_05960"
     CDS             complement(21950..24094)
                     /locus_tag="JA154_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(23678..23680),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HAT7995406.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(24345..25403)
                     /locus_tag="JA154_05965"
     CDS             complement(24345..25403)
                     /locus_tag="JA154_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAT7995407.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(25614..26273)
                     /locus_tag="JA154_05970"
     CDS             complement(25614..26273)
                     /locus_tag="JA154_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7995408.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT         7861 a         4842 c         2932 g        10697 t
ORIGIN      
        1 taattatgaa aaacaaataa ttgctttgga gattattaat atcatttttt agcaaaaaaa
       61 agtaaaatgg catagcctca aagctacacc aatttactaa aaattatatc attattaaca
      121 ttaattttaa acatatacgt ttataagttt tcctcatatt ttaaaattct tttaattaat
      181 ttatctctta atttccctac ttcatcaaat ctcatattag gactatagta actttctata
      241 atatcatgtt tagacttggc tctcttaaga tttagtactg cccaacaaat taaatcatct
      301 aaaacttttt catcaaattt aatatcttct tcatatttaa gtagtttttc tctatttaaa
      361 aactgattta aatcaattgt atcttcttct ttaaaagtcg tacttatagt tatgcctata
      421 tctaaatcag ttaatagtac agtttcaatt ttctctggaa taagtggata atgataaact
      481 tctacattta aacctttttc aatagctttc ttatatactt tgtttaataa tgtagttttc
      541 ccagttccta tttctccatc taaataatag actttcttta catcttttaa aatgttttct
      601 gtataatcta aacaccctac aggagttata gctgaaccaa ataaatgctt ttctcttttt
      661 agatttcctg tgcttttaac tccatcaaat aaccttttta taaattcttc tgtgaccaaa
      721 tttactttgc cataatccat acaatcagaa tatttttctt ctatgtctct aaagattggt
      781 tctgctgatt ttaaaaactt aaatgctctt ttaaatgaat tactaatatc tttatcataa
      841 actgatattt cttgtttatt cttttctaat tcttctgtat tccagtaatc acctaaatta
      901 attatctcat ccaaagctcc aggtagcttt gggtccatag tatgtggggc agttgcatcc
      961 ataagtacca cgcctaattt ctttataact aaaccatcca acgaacttgg gtcagaagga
     1021 caatgatata aatcaatatc atatcctcta tgcaaaaact ctttataaat ttttttcata
     1081 agtgaggatt ttcctactcc aggtccaccc tttaaacaaa aaactctttt tacattttca
     1141 ggaattatat agtcaaaaaa agaatatgag ccttgtgatg tatttccacc cgggaatgcc
     1201 ttctttacct ttccttccat attagcgcct cctcataatt tacaatatct cttaattaag
     1261 atattcatat tataagaatt ttattacagc tttctgattt tgacaaattt aaaacttgcc
     1321 atctaatact aataacaggt taattttatc taattattat agattctcat actgtgcctt
     1381 attctatcta taaatacaat ttaagtgtcc atattgaaat atttgtattg taatacagct
     1441 ggatattact taaatccaat tgtttccatt ataattttat gttaaaataa tattacaaaa
     1501 tacatctgtt tttcttcata aacgggtgaa attacctatc ggcggtaaaa gcccgcgagc
     1561 cttatggcat aatttggtca tattccaaag ccgacagtaa aatctggatg gtagaagaaa
     1621 atagtatatg agtaccttta tgtaatttta catgagtaat ctatacaaat ccttcaacta
     1681 ccgtatttat tcatgaaatt agacacattc aaggtaccta atatacaggt gctttttttg
     1741 ttgtttattt tacaattata tcgtacttat aaaatctatt aagattggag tgttatcatg
     1801 aaacaaaaat ggatagtatt gattatcatc tgtattggtg tatttatgtc tactcttgat
     1861 ggaagtatac taaatatcgc aaatcctaca attgctgctg actttaaaat taacatgtca
     1921 caaattcagt gggttgtaac agcttatatg cttgttgtaa cagctactat gttatttttc
     1981 ggaaaacttg gcgataaagt aggttcaaat cgtctataca cattaggatt ttttatattt
     2041 acaataggtt cttttctgtg tagtatgtca aacaatttat caacattaat ttcttcaaga
     2101 atctttcagg cagttggagc aagtatttta atggctactg ggcttggtat agtttccaat
     2161 gcatttccag caaatgaaaa aggaaaagct ataggaatca ctggagctgt agttggtata
     2221 ggaaatatga gtggtcctgt aattggaggt ataattttag aacattttgg atggccttct
     2281 atttttatta tcaatatccc tattggtatt atagcagtct ttcttggcat taaatttctg
     2341 cctaaacctg ttttggatga acaaaacaag agctttgata ttccaggtct tctacttttc
     2401 gcatcttgca ctacgcttat attacttgca atgaatgaga agggtaatac aagattgtat
     2461 ttaggtataa cagcacttat aatcttctta ttgttggcat taagagaagt aaaatttgaa
     2521 caaagcttta ttgacctccc tctatttaaa aatagaaact ttactgtagg caatatcatt
     2581 ggagtagctt gttattttcc tcaaatggca gtatcttttc tacttccatt ttatttagaa
     2641 caattaaaaa atttatctcc tatgatggct ggttatgtaa tgactgtaca tccacttatt
     2701 atggtactta tagcacctat tgcaggttca ctttctgata aacacggtgc aaaaaacatt
     2761 ctaacagcat ctttttcatt tatgaccatc tcactagttg gaatggcact tttaaaagca
     2821 gattctccat tgtacctttt aattgtatgt ttagttattt ttggacttgg gctaggagca
     2881 tttagttcac caaacaatag tagtatttta gcagatgttc ctcctcaaaa acaggggtac
     2941 ggaggcagtt ttcttgcaac tattcgtaat ttatcatttg ctcttggaac agcatttttt
     3001 agtagttttt ttgcacaaag tcttacatac aatcaaaagt ttaaaagtca cacatcagcc
     3061 tatgttatag ctagcaatca gtcttattgg attgcagcat cagtatgttt tgtagggctt
     3121 atactaactg ttttctttat gaggaaaaca gataaatcta tttcttaaat gctatttatt
     3181 actgactaca taaatcaata attttttagt ttaactattt tatactaatt ttcccatacc
     3241 ttactaaaat ttattagtat ttagcaaaat tactgatatt tattgtttat aaacaataag
     3301 gatgccaaaa aaaagttcat taaaactatt ttaaggcatc ctatatttta ctttaatatt
     3361 aatataatta aaactaacta ttattacatt tattattcaa ataaatctta tgtcaatatt
     3421 taacatttta aatttaatct actgttattt catatccatg ttgattgtat tgatttacaa
     3481 tctcttccca taattcttca acaccagtct tttttagtga agaaacagga attacttttt
     3541 cactactttc catttgcaat ttttttctta ttatacttat gtgtttttga tattgtcctc
     3601 tagatatttt atctgcctta gttgcaataa ctacacagtt atatccaaag tgctttatcc
     3661 attcatacat aagcttatca tctgctgtag gctcatgtct aatatctaca agtaggaata
     3721 tagaacataa ctcttgtcta ctttctagat atcgttccat tatacctccc cacttttctt
     3781 tttcagactt agctatcttt gcatatccat agcctggaag gtctacaaaa taaaattcat
     3841 tatttattag ataaaaattt atagttctag tttttcctgg tgtttgactg gttctagcaa
     3901 agtttcttct gttaagcaat gtatttatta ttgatgattt tccaacattt gacctaccag
     3961 ccaatgctat ctctgggatt ccttctgctg gatactgtga tttattcaca gcactcatag
     4021 ttatctctga acttctaatt ttcattattt tcaccactct ttaataaagc ttgttctaat
     4081 acttcatcca tatgttctac aagcacaaac tccatttttt cttttacatt ttctggtatt
     4141 tcatctaaat cagcttcaca ttctttagga attaatactt ttgttatacc agctctatga
     4201 gctgctaata acttttcttt aactcctcct actgctaaga ctctacctct taaagttatc
     4261 tctcctgtca ttgctatatt tccaggcaca ggtcttttag taagtgctga gattactgca
     4321 agagccatag ttataccagc tgatggtcca tcttttggaa cagcaccttc tggaatgtgt
     4381 atatgaatat cattcgtctt ataaaattca gggtctatat caaacttgtc cactattgac
     4441 cttatataag agatacctgt cttcgcagat tccttcataa catctcctaa ttttccagtt
     4501 aatacaattt cgcctttacc tttaagtacg tttacttcta cttcaagtgt aactccacca
     4561 acttctgtcc acgcaagacc atttactaat cctatttgag ggttaacttc tgcaagttga
     4621 tacttaaaca tgtcttttcc aagatatgtc tctaggtcag acttatttat aacaacctct
     4681 tcaagagttg ggtcttcaac gtattttttg gcaacctttc tacaaatctt accaatagtt
     4741 ctttctagtg ttcttacacc agcttctctt gtataatggt tgattatact tcttaaagtt
     4801 tcatcatcta tttttataaa gttttctttt aaagcatgtt ctttaatttg ttttggaagt
     4861 aggtattttt ttgctatatt taatttttct tcttctatat atccagatac ttctattact
     4921 tccattctat ctaataatgg tcttggtata ttacttagac tatttgctgt tgtaacaaat
     4981 agtatttttg ataaatcaaa aggtatttct aagtaatggt ccacaaaatc cttattttgc
     5041 tctgggtcta atacttctag cattgctgaa gctgggtcac ctttataatc tgctgccatc
     5101 ttatctatct catcaaataa gaatacagga ttttttgttt gggcttcttt taccccatta
     5161 attattcttc ctggaattga acctacataa gttcttctat gacctcttat ttctgcttca
     5221 tctctaactc cacccaaaga tattcttaca aattttctat ttaatgctct ggcgattgat
     5281 tttactatag atgtctttcc tgttcctgga ggaccaacta gacatattat aggcccttta
     5341 agtgacttag caagagttct tatagctaga tactctagta ttctctcctt aactttttct
     5401 aatccataat ggtcttcatc taatatatct tttgccttag ttatatctaa tttatctttt
     5461 gtctctttat tccatggcaa tgagaatata gtatctaaat agtttctact tacagataca
     5521 tctggagaca tagaagatat ttttgaaaat ttatctatct ctttttctat tttctcttta
     5581 gttgtcttag gtgcttttat ttttttaagc ttttctctat actcatctgc ttcagagttt
     5641 atatcttctt cttcacctaa ttctttctgt atagctttta attgttctct aagataatat
     5701 tctttttgaa ccttattcat ctgttttttt actcttaaag ttattttctt ttctattttt
     5761 aatatatcta tttcctctaa taatatagaa taaataagtt caagtctttt tctaatatca
     5821 aattcttcta atatttcttg tttttggctt gattttagat atatgtttgc agctatcgta
     5881 tctataaatc tatctacatc ttctatatca gctaaagata ttaatatttc tggtgatacc
     5941 ctatttccaa tatttatata ctcttcaaag gcatcaaata catttcttac aaatgcttca
     6001 atttcaactt ctgtttcact atccaaatta tcactatcaa atactatttc ttctattact
     6061 gctctgaaat acccatcttc ttgttctatt ttctttactc taccccttga tacaccttca
     6121 accaaaacac gaactgtatc tcctggtaat tttatcattt gcttaacttt acagattgtt
     6181 cccacatgat aaaaatcctc ttctcctggc tcatctacct ctgcttcttt ctgagaagtt
     6241 aaaaatataa gttcttcatc catcatagct tggtctaaag ctttaagaga tatctctcta
     6301 cctatatcaa aatttaatat catatatgga aatatagcta atcctcttag aggaattaat
     6361 ggtaattcat gatctatttt agtataattt tgttccatca acattactcc ttttctagtc
     6421 taattgcttt gcttttaata ttatatattt gaacaagtta attttcaaca ttaatgataa
     6481 attcttagaa tactatcata ctatattatc tttttattat gatataatat taatattagt
     6541 atacttagat gttatttatc ttttggaggt ttctattatg ttgaaattgt ttttaacctt
     6601 tttaaaaatt ggtgcgttta catttggtgg tggatacgct atggttcctc ttatacaaga
     6661 tgaacttgta aacaaacaaa aacttttaga gcaggaagaa tttattgatt atttatccat
     6721 agctcaaagt tatcctggag tactggcagt taatatatct gtattattag gatataaact
     6781 ttatggtata ccaggagttc ttatatgtac cttaggctct gctttaccat cattttgtat
     6841 agtcttagtc ttatcgtatc tatactttaa aaattcatca tctaaaattt tagatggatt
     6901 ttttaaagga gttgcgcctg ttgtaatagc tcttatattg tattctttta cgagcatgtt
     6961 caagaaacta ccaaagtcta aaactaacat actactatta attatagcag ttttatctgt
     7021 tggtgtattc aatataagcc ctatttattt aataattgga ggaggtgtat atagtctatg
     7081 tcagaaataa tactactctt cttaatattc cttaaaatag gggcatttag ttttggtggc
     7141 ggttatgcca tgctaccatt tatccagcaa gaatttatat ctaaatatca ctttataact
     7201 aatcaagagt ttttggattt acttgctata tcacaatcta caccaggtcc tgtagcaata
     7261 aatagtgcta catttgtagg atttaagact gctggcttct ttggttctct agccgcaact
     7321 attggtgtta caatttttgc attaataggt ctaactatta tatcaaagtt attttctaaa
     7381 tttaaaaata ataaaaaggt agaaaactta ttgacagttc ttagaccaat tactctagga
     7441 ttcatattat cagcagcttt ttcatctgcc aaagaagtta attgggattt atatggaata
     7501 attgcattta tagcatcatt ttatctttta tataaaggaa aaattggttc cattgcaatt
     7561 gtattcgtct ttggtattct aggtatatta ctcacatttt tataataatg atacctattt
     7621 tatatttagc atattaacat aaaaaaagga gcttttaaag ctccttttca tcatcttatg
     7681 cactttcctc ttgatcctta agtactatga taggatttac tgagtcttcg ttcacagatt
     7741 tctctgttac tataaccttc tttatattat ctctagatgg tacttcaaac atagtttcca
     7801 tcattacgct ttcaactata cttctaagtc ctctagctcc tgtatttctt tctatggcct
     7861 ttttagcgat tgcacgaaga gcaccttctt caaattctag ctcaacatca tctaactcca
     7921 ataacttttt atactgtttt actagagcat ttttaggttc ctgtaatatc tgcattaatg
     7981 catcttcatc caacaactct aaagtagcta aaactggtat tcttcctata aactctggta
     8041 ttataccata ttttaataaa tcctctggaa gtactttttc atatagcttc cctaaatcta
     8101 attctttttt actttctatt ttagctccaa aacctagtgt tttatctcca ccacgttttt
     8161 gaattatctt ctctaatcca tcaaaagctc cacctaatat aaataataca ttagtagtat
     8221 ctatctttaa aaattcttga tgaggatgtt ttcttcctcc ttgtggtgga acatttgcaa
     8281 cagtaccttc taatattttt agaagtgctt gttgaacacc ttcaccacta acatctctag
     8341 ttatagatgg gttttcagat tttcttgcaa tcttatctat ctcatctata tatattatac
     8401 ctctttcagc tttttctata tcaaaatcag cagcttgaat aagttttaaa agtatatttt
     8461 caacgtcttc tccaacatat ccagcttcag tcaatgatgt agcatctgcc attgcaaaag
     8521 gtacatttag agttcttgct agagtttgag caagtaatgt ctttccagaa cctgtaggtc
     8581 ctaataaaag tatattactc ttttgaattt ctatatcctt actactagat tttttactgt
     8641 atattctctt ataatgatta taaaccgcaa ctgataaagc ttttttagct ttttcttgac
     8701 ctattacata atcatttaga atctccatca tttcctttgg ttttggtaaa gatgtagtat
     8761 cctcatctat agtatcttct atctcttctt gaataatttc gtcacaaagt tcaacacact
     8821 cgtcacaaat atatacattt ggacctgcaa ttaatcttct tacttgatct tgatttttac
     8881 cgcagaatga acactttaat tgtcgttttt cttcatattt agacatatta acacctctct
     8941 ctatctacgg tctctttgta aatacttcat ctattaaacc atattctttg gcttctagtg
     9001 cactcatgaa gttatcacgt tcagtatcca ttttaatttt ttctaatggc tgaccagttc
     9061 tttcagataa tatctcattt aaagtttctt ttatttttaa aattctcttt gcatgaattt
     9121 ctatatctgt tgcttgacct tgagctccac ctaatggttg atgtatcatt atttcactat
     9181 ttggtagtgc tagtcttttt ccttttgctc ctgctgccaa caagaatgct cccatagaag
     9241 cagccatacc tatacatata gtacttacat ctggtttgat gtattgcata gtatcatata
     9301 tagccattcc agaagttata cttcctcctg gagaatttat atataaatgt atatctttgt
     9361 ctgggtcttc agcttctaaa aataatagct gtgctactat aagccctgct gtggcatcgt
     9421 taacttgatc tcctaagaag attattctgt cttttaatag tctagaaaaa atatcataag
     9481 atctttctcc tcttcctgtt tgttcaacaa ctacaggtac taatgccatg gtttcatacc
     9541 ccctttattc tatataattt acagtagctt tttttaagct actgtaaatt attatacgca
     9601 tttaactaaa ttatatttgc acatcattat atactaaaca tttcataacg tcaactatat
     9661 tagttagcgt ttattttatt aagctattgt tgcattatca actaataaat ctattgtttt
     9721 tcttattttt atttgacctt ttatatcttc tttgtcggca tctcttaaag aagcttctat
     9781 tttttctact tccatattgt aagcagaagc cattttttct aactcagcct taaattcttc
     9841 ttctgttgct tcaattcctt cagctttagt tattgcttct aatactaaag aagacttaac
     9901 taatttttta gcatcttctt ttctctcttc tcttaattct tccatagttc taccagtcat
     9961 ctctagtaat tgttgtaatc caaatccttg atattgtaat tggtagttaa gttcatttaa
    10021 catattatct atttgatgtt gtatcataac ttctggtatt tctatttctg tattttcagc
    10081 aactttttct actactgaat ttctagtttc tgcatctgct ctatttttag cttcttcttc
    10141 taacttagct cttacatctg cttttaattc atctaaagaa tcaaattcac tagtatcttt
    10201 agcaaattca tcatcaagtg cagataattc ttttactttt acatcattta tttttacatt
    10261 aaatacaact ggttttccag ctaaatcttg tgagtgatat tcttctggga aagtaacatt
    10321 aacttctact tcttcaccag cttttttacc tactaattgt tcttcaaatc ctggtataaa
    10381 tgtgtttgaa cctataacta agttataatt ttcaccttta ccaccatcaa aagctacacc
    10441 attttcaaaa ccttcgaagt ctattatagc tgtatctcca tcttctaaag ctttgtcttc
    10501 tacacttact aatctggcat ttttttcaac catttcttgt agtttagcat caacattttc
    10561 gtcacttaca gtattctcaa ccttagctat ttctataccc ttgtagtttc ctaattcaaa
    10621 ctctggttta acttctacat taagaaccat aaccaaccca ttgtctttac ttatttcttc
    10681 tatatctaaa tctggattgt ctattgggtc tatgtctaat tccttgatag cttctggata
    10741 aacttctggg aataatattt ctatagcatc attgtagaat ataccttttc cgtattgagt
    10801 ctctataaca attcttggag ctttaccttt tctaaatcca ggtatattgt atttatttct
    10861 gcttttatta taagccttat ttactgctgc ttcaaattta ttgttatcta ctgttatttt
    10921 taaagtaact ttattaccct cttttttaat tagttctgct ttcatccttc taatcctcct
    10981 agtgatattt atgtaatacc cgataacggt attataattc attctattta taattcaacc
    11041 acttaattat atcacataag tttttttatt tacaagtgtt ccattaatat cgttgtactt
    11101 atttgataaa atttaatttt ttgagcgttt tttgccattt ttttagataa atacaccttt
    11161 atatgcgtat ttcttcaatt tttatgtcat cttcttctat atccataatg acaaaacttt
    11221 tatacgatac tcctcttgga agtgaagtgc ttccaggatt tatgtaaagt acaccatctt
    11281 taatttctct aaatggggta tgggtatggc caaatataac tatatcagca tcaacttcag
    11341 ttgccttttt ttctaacata tgagttccat attttacgcc atacttatct ccatggcata
    11401 agaatatggt tttatttgcc acttcaaaga caacttcttc ctcaacagca tcaaagtcac
    11461 aattaccttt aactgcaata atacctacat tagtcatgct atgaatatat cttgaatcag
    11521 taaagttatc tcctgcatga attattaggt cacattcttt caaatatggt attgtttttt
    11581 ccatattttt aatcatcata tgtgtgtcgc ttactatgcc tattttcatt acttcactac
    11641 cctttttata aactcttgtt tcataagttt tagtgcgttt gctctatgac tgattgaatt
    11701 ttttataaca ctaggtattt caccaaaagt cttatcatat ccttttacta taaataagct
    11761 atcatatcca aaaccatttt tacctttttc ttcaaatcct atcattcctt cacaaattcc
    11821 tcttactaca aattcttttc catcaggaaa aactactgct attgcagaga cgaatcttgc
    11881 atttcttttg ctcattggta tattttgcat agcttttaac agtttttttc tattttcttc
    11941 atctgttgca tgttctcctg catatctagc tgaataaaca ccaggttttt tgcctaaagc
    12001 atctacttct aagccagaat catctgatat tgctataagt ttagtctttt ttgctattgt
    12061 tctagctttt atcaacgcat tatgctcaaa tgtttttcca tcttctacta tctctatacc
    12121 tgctaaatca acatctttta aagagtacac tttatattca aaatctttta agatttcacc
    12181 tatttcttct agcttatgag cattattagt agcaatcact acttcattac cataatccat
    12241 acccaatatc tcatctgata tttcacctaa agcttctctt tgaagcctta ttaattcttt
    12301 attccctttt tcacctaact ctaacaataa atttaaatct gctctcgaaa aaggtttctc
    12361 ttctcctgtc ccctgtactt ctacaaactc acacttatca gtcattatta tattcatatc
    12421 tacctgtgct ttagaatctt cttcataaca taaatctagc atgtgtattc catttacaat
    12481 accaacacta attgcagaca caaaatttgt tattggcatc tcttttatta tctttgcttt
    12541 atataattta tatattgctt ctgcaactgc tacaaatgcc cctgttatag atgcagttct
    12601 agttccacca tctgcctgaa tcacatcaca atctacccaa atagttcttt ctcctaattt
    12661 attcaagtct acaacagacc ttattgctct tcctattaac ctctgtattt cttgtgttct
    12721 tccatcaatt tttcccctag atgcatctct tatctttctt tgttgtgttg ctcttggtag
    12781 catagaatat tcagcattta tccatccagt accactattt cttaaaaaag gaggaacttt
    12841 ttcttccact gatgcagtgc atataacctt tgtctctccc atttctataa gaacagaacc
    12901 ttctgcatac tttgtataat ctctagttat ctttacaggt cttatctgtt cgttacttct
    12961 atcataactt ctggtcattt tttatatcct ttctcatatt ttttattttt tataaattta
    13021 tttttataat ttttaatttt gtcaaatatt aattattgtc atttttcgta atcttattag
    13081 cttactgaac tattctatca ttaattatag tagttttcta ctaaattaag cgacttttta
    13141 actctctaat acagctttta cttcatcttt cgatgataac tttaataatt ctaaaaatct
    13201 ctcttcatct ataactttaa ttcctaaatc atttgcttta gtaagtttag aaccagcttc
    13261 ttcacctgcc aaaacaaatg ttgtcgattt actaacacta gatgtagctt ttccacctct
    13321 tttttctatc atatcttttg catcatttct ttttaatgta ggaagtgtac cagtcaatac
    13381 gattttcatc ccttcaaata ttttttctac tttttcacct gtattatcca atgattttgt
    13441 atttactcct acatttttta acttgtttat aacgttcata tttttgtctt ccttaaaaaa
    13501 ctcgacaacg ctgtttgcca ttatacttcc aaattcctct aattcttcta gttgctcact
    13561 agttgcagaa attacttcat ctaaatcctt aaagttatat gcaagtatct tagctgcttt
    13621 aactcctata aatttaatac ctaatccatt tataagtctc cataaatcat tttcttttga
    13681 tttttcaatt gaatttatta aattgtcagc agatttttcg cccattctct ctaggtcgac
    13741 tacatcttcc ttttttatat aatataaatc agatacatct tgaataattt tttcatttaa
    13801 tagcagtgta attatagatt ctcccaatcc atctatattc attgcatccc ttgacacaaa
    13861 gtgtataatt ccccttctta tttgggctgg gcaagacata ttgatacatt ttacagcagc
    13921 ttctccttct aatcttattg ttggctctga acaaactgga cacttttctg gcattttaaa
    13981 atctatttca tctccagtac gttcttcttt tataacttct aaaacttgag gtattatgtc
    14041 ccctgctttt tgaactaata ctgtatcatt tatttttata tctttttctc ttatataatc
    14101 ttcattgtga agtgtagctc tacttacagt agtacctgca agtctgactg gttcaagaat
    14161 tgctgaaggt gttattgtac cagttctccc aacttctact attatatcta ctattttagt
    14221 tttcttcttt tctgctggga atttatatgc aattgcccat ctaggacttt tggctgtata
    14281 tcccatttcg tctctttgtt ttatattgtt tacttttatt accataccat ctatttcgta
    14341 gtctaaatta cccctgttat ctgtccagta ttcaatatgc tcaataactt catctatact
    14401 tttacaaact ttataactct gacttactga aaatcctagt ttttctaagt actctagtga
    14461 ttcactgtga ctttctaaat cgtattcttc tatattttcc atattaaata caaatatatc
    14521 taatggtctc tttgctgtta gttttgggtc taattgtctc aatgaaccag ctgcaagatt
    14581 ccttggatta gcaaataatg gctgttcttg ttcttcttgt tgttcattta cttttctaaa
    14641 attatctttt gatatatata cttctcctct aactattagt tcttttttat catttatctt
    14701 aagaggtatg ctttttactg tcattaagtt ttgagaaata tcttctccta caactccatc
    14761 tcctctagtt gctccttttt caaactctcc attgttataa gttagaccta cagaaagacc
    14821 atctatttta aattcaacaa catattcaac attttctccc acatattctt gaactctctt
    14881 atcaaaatct ctcaaatctt gctctgaaaa agcatttgat aaactcaaca taggagtttt
    14941 atgtacaatt tgattgaact tatctaatgg cttacctcct accctatttg ttggagaatc
    15001 aattctcttt agttctggat gttcttcttc taaagaaatt aactccttca ttaacttatc
    15061 gtattcataa tctgatattt ctgggctatc ttgattataa tacctttcat tatggtagtt
    15121 tattgtctct ataagtttat taatttcatc ttttacgctc actttttgca cctcttcata
    15181 tttttaaaat taaattattt tgaaatttcc ctataattaa ttatctccta tttatatatt
    15241 ttttagtata tcactttttg ggctttaaat ttattacaaa aaaaactctt attagtatct
    15301 ttaatacgac taataagagt ttttattttt atcaaaattt tttattttta atagcctctt
    15361 gatatgtcta caacattatc tacaggttta ttctccatta tatagtgttt caaattatta
    15421 taaaccatat taaatgttct ctctttgttt ttatctgaca cccaagaatt atgaggagtt
    15481 actatgacat tttcgcactc ccacaactta ttgtccttgt ttaaaggttc actttcaaat
    15541 acgtctaatg ctacccctct aaatttctct acacattctt ctaaatcttt ctgattaact
    15601 gtatttcctc tacctacatt tataaataca gaaccatctt tcataagttt aaacatatct
    15661 ttgtttacta ttccatctgt actttttgta gaaggcattg ctacaactac tatatcacat
    15721 tcttcaaata caatattcat atcttcagta gcaaaacact tatcaaagta ttttttacta
    15781 cttccatcag tattgacacc ccatatttct acaccaaaag cttttaatct ttttgctgct
    15841 tctgaagata tagtacctgt tcctaaaaat ccaactcttt gtccatacaa ttctgataca
    15901 gaaaaatcca tcttccattc tttattttgt tgttgtttaa aaaattttgc actattttta
    15961 tatatttcta aaatatacaa tacaatactt tcaccaatag gtacactata tccgcctcta
    16021 ttattagcta tcttaatatt tctcttcaat acctcttctt ttggaagttg gtctatacct
    16081 acactagttg tctgtatata ttgtaaattt ttcatcttag aaatatctaa tctctcaaat
    16141 ggattgtatg taactagtac atctgctgta tttacatcct cattattttc aatactatct
    16201 tcagagtaat gtatgacatc atagcctaat tctcttattt tatcaaattt ttcttttcca
    16261 taatttattg taaataaaac tttcatctca aatctcctcc atctacccac atttaaaatt
    16321 ttatattcta aaatataata tgatactcaa tgtacattag ttaaaattac tataagatat
    16381 cttaaattta cttatgttca attgtttttc ctaaagacat agcattacgt tcttgaacta
    16441 aattttctat taaattattc aatgtccaat caatagatgt ctctgttacc atagctgtaa
    16501 aaggtctatt tattctcact ttttttagcc cttctaaaaa tgcatttact tgtgtgcttg
    16561 gagtgttatt aaatattaca gacttactct taatcccatt ttcacattca tttgatatat
    16621 ttccatctaa aatgtctctt attatattct ctccattttt atcacttaag atgatatggt
    16681 ctcttattcc agcaaagtta cttacgtttt taattaatga taattctttt ttattaaagt
    16741 ttacaagcat aatacacttc ctgttgtgta ttttatctag gttattatta tttatttttt
    16801 caaatgacat tttatccatc ctttcattta tttatatact ttaatttacc ccatacattt
    16861 aaaataatca atttttatag ttaattttta tattaatacc caaattatat ttaaatagtt
    16921 gggctatcca aaactaatat ataaaattag aataattaga ttaatatcta caaatcataa
    16981 cctaaaggag agataccatg aatacattac taaacaataa atttagcatt aaaaactttt
    17041 caaatgcttg ggttatagta cttggttgta tgttgataca agctgttcca tttagtattg
    17101 tagcaagtat acagcctcaa tttataagtt atgttgtaga aggagaaggc tttagtttag
    17161 cttctttttc tctaatattc actattggta ctattgcctc agcaattgca tcacctttta
    17221 taggtgtatt attttctaaa gtcaatatta agatactata tttattaggt tgtataattt
    17281 ctagtattgc ttttgctact ttttctatat gtactgaact atggcaattt tacactgtat
    17341 catgtattgt tcaaattggc gctacaatta tatctgctat aggaatacct gttttaataa
    17401 gtgcttggtt tgatgaagaa agcaaaggaa aagctttggg ctttgctttt gctggtggtt
    17461 ctattggtag tatatttcta caaccacttg cagttcgctt attagcaact caaggataca
    17521 gtcgttctta tctaatattt ggaattatag cattaatagt tggtatacct gtaatattat
    17581 tcatactcag gatgcctaaa gatattaatg aaaaggtaaa atctaaaggt aaaaaagaga
    17641 aagcatctga tacaatttct ccttcagtat ggggttatac attaaaagaa gctaaaaaaa
    17701 ttaaatattt ttggattatg actattggat ttatatttgt tggtatatat gtttcatcat
    17761 tatccgtaca atatccaact tatcttagaa attatctaaa tcctattcta ataggaacag
    17821 taggctccac atttgcttta tttgctctca ttggtaattt atttggagga ttattgtttg
    17881 ataaattcgg tcaaacaaag ccacttatta tatcttttgt attggttctt atagcaactt
    17941 tatcattact attggtagat aaatcttcta cttttgcatt tgtatttgcc attttgaaag
    18001 gtctttctgt atttgcttat atgattggtc ctgcttattt aactggctct ttctttggtc
    18061 aaaaggagtt tggtagcata tatggaatcg tacaattaat ttttgcagtg ggatttgcat
    18121 taggttcaac attatttgga ttgttagttg ataattttgg atacagattt tcttggtcta
    18181 ttataatttt atcagttata attgcctata ctttacttat tataacttct ataggaatga
    18241 aaaaattgaa taaagaaaga ttagataaac taatgcaata attgattttt agatatataa
    18301 atattataat aaaaaagatg gagcttttaa tcataaataa aactccatct tttttatatt
    18361 acaatatagc taattttctc ttatttttaa ctttactatc tatatcctca tccttaacta
    18421 tagtaaatgc cttagttgga caaacattta cacatgcagg cccttctggc gattctatac
    18481 aaaaatcaca cttatttgct gtcattcttt ctctaaagca aagctttcct tcatctacat
    18541 cttttaatgt aactatgtct attgcaccta taggacatgc taacatacaa gtcttacatc
    18601 ctatacaatt ttcttcattt atttttattg tgttcccaac cctaactata ccaccatttg
    18661 gacatgcctt tgcacaagga gcatcttcac aatgtctaca ctgtattgga gcacttactt
    18721 cttttgtctt tatgacagaa agttttggat taaaatttat ttcatctaga ttttggtaaa
    18781 aaatatcttc atctgaatgt gcaactacac atcctatcat acaagttcta catcctatgc
    18841 atttatcagg gtctgctata acaaaatagt tcatcactta gccacttcct ttcctataca
    18901 atatgtagtg tgtaatagat gatgagattt ttctcctaat ggttctacca gatattcatc
    18961 atacagtttt ttaatatcag gattctcatg agattttcta attgctaaat gttcatcatg
    19021 cacgtatgtt gcttttgttc tattttcata agccaattcc ttatattctt ctagtaaaat
    19081 ttttggttga cctccaccac ttacacaccc aacaggacaa gtcataactt caataaaatc
    19141 tacatctaat tttccttttt ccaagtcttc aagcactggt attacatttt ttaatccact
    19201 tactactcca actcttaagt ctaagtctcc tattttaata gttgactttc taaaaccatt
    19261 ttctcctctt acttgtttta cttctacatc aggaatactt tctccagtta taagctcata
    19321 tcctgttctt atagcagctt ccataacccc accagtaaca ccaaagatag ttccagctcc
    19381 actataacta cctaaaggac tatcaaattt ttcttctctt aaatttttaa aatcaattcc
    19441 catgtctttt attaaataag ctagttctct agtagttaat actacatcta catctctata
    19501 tccactagaa tccatttctt ctctatcaca ttcatatttt ttacatgtac atggcattat
    19561 agaaacacta aatatctttg atgcatctac attatttatc tctgcaccat aagttttaaa
    19621 tattgcacca cccatctgct gaggtgattt acaagaagat aaatgattag ttaatttagg
    19681 ataatttttt tccatgaatt ttacccaagc tggacaacaa gatgtaaaca ttggaagagt
    19741 tccaccttct gtcactcttt taattaattc actaccttct tccattatag ttaaatctgc
    19801 tgcaaaatta gtatcgtaga ctctatcaaa atttaaagct ctaagtgctg ctgccatttt
    19861 cccagcacct aagtatccta aataatatcc gaaatcttct cctaaagcaa ctctaactgc
    19921 tggtgcacat tggaccatag tgaatacatt tgggtcactt aaagctttct tcaccttatc
    19981 tatatcacat acattgtatg ctgcaaatat aggttcattt atagactctg gtatctctct
    20041 ttcttgtttt ttctcttgta aaaactcaaa tccttcatct ataactgaag cataagattt
    20101 acaagtttga acacattgac cgcacataac acatctatct aaatttatct cttgtggctt
    20161 tccctcttca ccttcaatag catttactgg acagaccttt gaacattctc tacatccagt
    20221 acataaatcc ttatctatat ttattatcat aaacgtcacc tacccctgta tcctttattt
    20281 atttgttaac aataaactaa gtgcagcttg tctattctta gcttttttgt catctattgg
    20341 agtaacaagt tttaatgctt cctgaggaca tgcacttata caagctattt tttcattgtc
    20401 tttacataag tcacatttat aagctatctt cttattttca tctaaaatta cttgttctac
    20461 ttctttacca tcttcatatt gtggaagtaa gtctatagct ccaaaaggac atgctagcag
    20521 acaagtttta catcctatac acttttcttc atttacagac ataatattgt tttctttaac
    20581 tatagctttt tgtggacatg agtttaaaca aggagcatct tcacactgtc tacactgtat
    20641 tggcatacag aaattgtcac cttttactaa gtaaagtcta ggtataacag gtatatcaac
    20701 tgttccaact gtataaccca cattattatg ctgattatgc attgtaaaac aagctacttc
    20761 acaagctcta caacctgtac atttgtttgg gtctgctatt acaaaagagc ctattttatt
    20821 tttcatatta tcccaccttt caatttgtat atttccaacc ctctagttat tttcatcata
    20881 tgtctactta ctttcatcat atatatttat atatttcact attttctcag tacttatatt
    20941 tcgtttacat tttttgcaca aattgtatgt gttattatac accttatcag tatcatcaat
    21001 taatactcca cagatttcac actttgaata tctttgctct tcttttttag tacacatata
    21061 ttcattctca ctttttgata actctctgtg gatttgcata aatattcatc cgtcttcctc
    21121 ttacaaatcc aataagcgtt atatttaatt ttttagaaag ctctattgat aaattagtcg
    21181 gtgctgattt tgatactaca ataggaattt gagtcatagc tgcttttaat atcatttcca
    21241 atgatattct tccacttaca gccacaattt tatcttttaa tgttatgtca tttaaaacac
    21301 aaaatccaat agccttatcc attgcattat gccttgctac atcttcacaa gtgactattt
    21361 ttttatcaaa attaaataca gatacgctat gtaccccacc agtattttta aataattctg
    21421 atgaatttaa atttctatcc attatactgt aaatcgtatc ataatttatt tttatattgc
    21481 tctttacagg agcacatgtt atatagtcta gtgaatttaa aaatataatt tgctcatctt
    21541 gatttttttg tttctcttga atagttactt ttgcaaaacg ttcactctca tttatctcaa
    21601 attttaataa gctttttttg ttttctatgt atccttttgt tttcaagaat cctactataa
    21661 gttcctctaa attatatggg gtacataaaa atgtatttat atatttatca tttaaaataa
    21721 aactaagtgg atattctgca attatttctt cattatctat tgatacttca tgttcattta
    21781 ttttgcttac ttctacatta aaagaattta aatatttgta gtccactaga ctataactat
    21841 tttcaaaaac tttatcctta ctattttctt gtaatttagt ttttgagtat atattgttca
    21901 taaaatatta gcctttctgc cttttataaa tcttaaatct aataaatcat tagatatttg
    21961 tagctgtcat tttgtctttc aaactttgat actcttctct tacacatttt tctgctaatt
    22021 cttggtcttc aagtttttca agtttaacag cacaatattt atactctggt gttttagata
    22081 taggatctaa atttgctatt gtaagttcat tgcaagctcc aacccaccac tgataagtca
    22141 tatatgttgc accttcctta actctatcag taactgttgc tcttgttatt acacttcctc
    22201 ttcttgaact aattctaact aattcatcat cttcaattcc taatttttca gcatcattag
    22261 aatttatttg aacacgtcct ggttcatctt ctaatgaact aagtgttcta caattaccag
    22321 tcattgttct tactgaatag tgtccaactt ctcttacagt acataaacta aatgggtatt
    22381 catcatcttc aacttccata gggggtctcc attcagcagc gaacaaatta cctttaccat
    22441 taggagtaga aaacttttga ccttcataca gatacatagt tcctttatct tccatacttt
    22501 cactcttaca aggccattga acgcatcctt gagtttctat cttttcataa gtagcaccta
    22561 agaaacttgg acataattgt ctaagttcgt cccatatttc cttagtattt ttataattca
    22621 taggatatcc cattgctgta gaaatttcgc ttataatttg ccagtctggt tttacatctc
    22681 cttgtggctc tatagctttt ctcatcaatt ggaatcctct atctgcacat gtatatactc
    22741 cctcatgttc tccccaagaa gtagctggaa gtataacatc agcgtgaagt gctgtcttat
    22801 tcataaatat atcttgaaca atgacaaatt ccaattcatc taatgcttct ctaacttctg
    22861 atgcatctgg gtcactttgc actgggtctt ctccaaatat ataataagct ttaagctttt
    22921 tctcttttag tactaaattt ggaacttgag ttaaactata accattatta ggagataatt
    22981 ttacccccca agctttttca aacttttctc ttatcttatc atcagttaca ttttgatagc
    23041 caggatatac attaggaaga gctcccatat cacaagctcc ttgaacatta ttttgtccac
    23101 gtacaggtcc tattccaacg ctttctctac caaaattacc tgtaagtaga gcaattgatg
    23161 caagtccctt aactacatca acagcttgac caaattgaca gacacccatt ccataaagta
    23221 tcatagcttt tttacctttt gcatattctc tcatagcttt tcttataagc tcttctggga
    23281 ttcctgtaat cttttcagca tatttagctg tatatggttt aacaatttct ttatactcat
    23341 caaatccttg tgtatgattt tgaacaaatt ctttattgta aagaccttct tctattaata
    23401 catttccaaa agcatttact aaaaccatgt ttgttccacc ttttataggt agatgtatat
    23461 cagcaattct tgcagattca gttactctag ggtcagtaac aattaattta gcaccatttt
    23521 ttttagcttt tactattcta tttgccacta ttggatgtga atctgctcca ttatatccaa
    23581 atataaataa aacatctgca ttttctatct ctggtataga attagacatt gcaccactac
    23641 ctaatgagta agccagaccg gctacagatg gtgcatgtca gacacgagca caatggtcaa
    23701 cattattagt tcctattgta gctctcataa atttttgcat tatatagttt gcttcattac
    23761 caggtcctct tgcagaacct gttcccataa tagaatcagg accatatttt tctttaattt
    23821 catttagtct tgaagctgta tagtttatag cctcatccca ttcaacctct tctagtacac
    23881 cttgttttct tatcattggc ttttttagcc taggtgttag aattttaggg tcatttagaa
    23941 aatcccatcc ataacgacct tttaaacaca aactttcttg atttgttcta ccattagccg
    24001 gttcagctct taatatctta ttatctttta ctactagata taattggcat ccagctccac
    24061 agtatggaca aactgttaaa atttttttct ccatacaatt ccccccttag atttatcccc
    24121 tgataagtaa aatttatctt aaagataatg agtgttttta taatatgcaa aatcataaat
    24181 attttgcaat actatctatt aaattcaaca attttctcac aattcctctt aaatttcaaa
    24241 aaaaatatgt cataataatc tttatatttc aaccatttaa ttaaaatatt ctagttctta
    24301 tgacatattt tttaatatac tcaatatata ttctttaaac ttatttagtt ttcacttaaa
    24361 acaatatttc tgccaattct atttttcatt ctatagtgag taattacagt aaatattcca
    24421 cttccaatca gaatcaaata gtacataaat ccaccatata aaaatactgc atatcccatc
    24481 aaaggttgag ggaatactga tttgaatata gtgaaaaagg ctaattcatt agctcctaca
    24541 ttcccaggtg ttggtattgg agatattgcc atatataaaa atgcttgtaa tgtcagtata
    24601 taaatatagc tataaccttg taaattaaat gctttatata tccaaaatga aacactaaag
    24661 taagcactta attgaataaa agttactatt atagttgaaa ttaatacctt tttattcttt
    24721 gcaaaaaaat ttattgcttt actataatca tctataaaac tttctatgga ttcaatctta
    24781 ttatctaaaa attttaaaaa cttaattttt gataaatgct ttatagtaat tctcataaaa
    24841 tattttattt ttttaggatt taaaataact aatattatca taattaagac aaatgaatta
    24901 atggctatcc ctagaaacac tagaggcata atcattttca tttgtttttc taacatagca
    24961 aaattcatta aaacaagtat tgtacaataa aaagtaacta ctatttggaa gataatagac
    25021 ttattggtaa caactgcact tgccttacta agtggcattt tacatttttt aagtacatat
    25081 atctgtactg gttggcttcc tgaagcaaaa ggagttatta aattataata aaatcccata
    25141 atggctagct taaaaccaat aaatctaata ttaactttgt gagtgctttc tataattatc
    25201 ttcattacta ctccttcaag cattatgtga cacattattg ccaaagcacc tatgaataaa
    25261 aacttcttat ctaccataac cataacattt gatagcattt taatatccaa tgttttaaac
    25321 actaggtaaa ttgttatacc tattaacaaa aataaaaatg tatactgtag taaactcttt
    25381 ctaatattct ttagtctatc cattagaaat aaccatcctt tgctcaagat ttatactaaa
    25441 tactatatta ttagtataca ctatgaatct taatatttta taacaaaata ccttacaatt
    25501 ttgtcattta caaaaaatat taatctataa attaaatttt aatatataga ttaatatttt
    25561 acaaacattt tgtagatata tataatattt tcactaaaca acacttatac ttattacacc
    25621 catttatacc ctatccccca tacagtttct attgtataaa tatcatattg ttttaatttg
    25681 cttcgtatat tttttatatg ctctgtgata gtacttatat cactttctcc atcaaaacca
    25741 tataccgact catatattct ttcttttgaa aatacttgtc ccttattttt tgctagaaat
    25801 tcacatatta gatattcact tttagtaaaa ttaatttttt taccatcaat tgaaacttct
    25861 ttgcctaaaa agttaaattg aacatttgag atagttaata aattcttttt ttctctactt
    25921 tctcgcctta aatgagcagt tatcctagcc cttaattctc ctacaccaaa aggtttggtt
    25981 atatagtcat ctgcacctat accaagaccg tacataatat cactttccat attttttgct
    26041 gtcaaaaaca gtattggaca atcaacctta tttcttatag ttttacatac ttcaaaacca
    26101 tccatcttag gcatcataac atctaatatt attagattaa aataattaaa atccatgttc
    26161 atagcttttt ctacatcact aattaccttt acttcatgtc catctctttt caacacattt
    26221 tcaatcaact ttaacatatc taactcatca tctactacta atatcttaga cataataact
    26281 cctccattta tccgtttaat agtattctaa tttatatatt taataatatc tt
//
LOCUS       DACULQ010000035        26156 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.035, whole genome shotgun sequence.
ACCESSION   DACULQ010000035 DACULQ010000000
VERSION     DACULQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.035"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(232..1761)
                     /locus_tag="JA154_05975"
     CDS             complement(232..1761)
                     /locus_tag="JA154_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAT7995409.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(1776..2255)
                     /locus_tag="JA154_05980"
     CDS             complement(1776..2255)
                     /locus_tag="JA154_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAT7995410.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2364..3332)
                     /locus_tag="JA154_05985"
     CDS             complement(2364..3332)
                     /locus_tag="JA154_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAT7995411.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3469..4239)
                     /locus_tag="JA154_05990"
     CDS             complement(3469..4239)
                     /locus_tag="JA154_05990"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090078.1"
                     /note="type III; catalyzes the formation of
                     (R)-4'-phosphopantothenate from (R)-pantothenate in
                     coenzyme A biosynthesis; type III pantothenate kinases are
                     not subject to feedback inhibition from coenzyme A and
                     have a high Km for ATP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HAT7995412.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4258..4848)
                     /locus_tag="JA154_05995"
     CDS             complement(4258..4848)
                     /locus_tag="JA154_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAT7995413.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5032..6000)
                     /locus_tag="JA154_06000"
     CDS             complement(5032..6000)
                     /locus_tag="JA154_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S58 family protein"
                     /protein_id="HAT7995414.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(5993..6970)
                     /locus_tag="JA154_06005"
     CDS             complement(5993..6970)
                     /locus_tag="JA154_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAT7995415.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7285..9279)
                     /locus_tag="JA154_06010"
     CDS             complement(7285..9279)
                     /locus_tag="JA154_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HAT7995416.1"
                     /translation="MYERRASILNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLK
                     FSEVYRELTDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKA
                     GKLTFGGEAKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVH
                     KDDEKTRVTFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKT
                     YLSRAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVG
                     RKRGAGLGGGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDR
                     QVVVGTPDVKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTA
                     RKREKKIKMETIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPV
                     HQVTIVPRGRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASN
                     DLERVTATARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETK
                     RIVDGAYDKTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTE
                     ENSSKEVEEQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9289..10677)
                     /locus_tag="JA154_06015"
     CDS             complement(9289..10677)
                     /locus_tag="JA154_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)-lysidine synthetase"
                     /protein_id="HAT7995417.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(10860..11273)
                     /locus_tag="JA154_06020"
     CDS             complement(10860..11273)
                     /locus_tag="JA154_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAT7995418.1"
                     /translation="MSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVIYKEKE
                     EDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDTRELDI
                     NIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11305..12111)
                     /locus_tag="JA154_06025"
     CDS             complement(11305..12111)
                     /locus_tag="JA154_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAT7995419.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12313..12819)
                     /locus_tag="JA154_06030"
     CDS             complement(12313..12819)
                     /locus_tag="JA154_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HAT7995420.1"
                     /translation="MDRWSKLMATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIA
                     KKEVINLSDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQK
                     NAFSVVLDGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMR
                     IGNHVFAK"
     gene            complement(12849..13508)
                     /locus_tag="JA154_06035"
     CDS             complement(12849..13508)
                     /locus_tag="JA154_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HAT7995421.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(13653..14309)
                     /locus_tag="JA154_06040"
     CDS             complement(13653..14309)
                     /locus_tag="JA154_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7995422.1"
                     /translation="MDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLAEQLGVSRT
                     PVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYLAAERISDE
                     DIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINSLWEQVYRF
                     RVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMIDKAMISKDL
                     "
     gene            complement(14317..15207)
                     /locus_tag="JA154_06045"
     CDS             complement(14317..15207)
                     /locus_tag="JA154_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAT7995423.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15325..16875)
                     /locus_tag="JA154_06050"
     CDS             complement(15325..16875)
                     /locus_tag="JA154_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAT7995424.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(16969..17235)
                     /locus_tag="JA154_06055"
     CDS             complement(16969..17235)
                     /locus_tag="JA154_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995425.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17383..18243)
                     /locus_tag="JA154_06060"
     CDS             complement(17383..18243)
                     /locus_tag="JA154_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HAT7995426.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18327..19265)
                     /locus_tag="JA154_06065"
     CDS             complement(18327..19265)
                     /locus_tag="JA154_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HAT7995427.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19375..19536)
                     /locus_tag="JA154_06070"
     CDS             complement(19375..19536)
                     /locus_tag="JA154_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995428.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19546..20043)
                     /locus_tag="JA154_06075"
     CDS             complement(19546..20043)
                     /locus_tag="JA154_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HAT7995429.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20158..20868)
                     /locus_tag="JA154_06080"
     CDS             complement(20158..20868)
                     /locus_tag="JA154_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995430.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21044..21574)
                     /locus_tag="JA154_06085"
     CDS             complement(21044..21574)
                     /locus_tag="JA154_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995431.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21591..21821)
                     /locus_tag="JA154_06090"
     CDS             complement(21591..21821)
                     /locus_tag="JA154_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995432.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(21836..23230)
                     /locus_tag="JA154_06095"
     CDS             complement(21836..23230)
                     /locus_tag="JA154_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HAT7995433.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23356..24609)
                     /locus_tag="JA154_06100"
     CDS             complement(23356..24609)
                     /locus_tag="JA154_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7995434.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(24660..25814)
                     /locus_tag="JA154_06105"
     CDS             complement(24660..25814)
                     /locus_tag="JA154_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase"
                     /protein_id="HAT7995435.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
BASE COUNT         7988 a         5064 c         2599 g        10505 t
ORIGIN      
        1 atattttcat aataaattac tttcaatcat attttctaaa tataaagtct caatcaacaa
       61 aaactaatat caatttctaa cgtttgttgg taatttataa attaaatatc taaaagaatc
      121 tttgaagcat ataaaaagca ttctacactg acattttaaa taaaatttag ctatttattg
      181 ataatttaat agctaaactt tatttaaaac ttagtagaat gctaatattt cttagttttc
      241 ttcttcttta tttggattgt tatcaatagg tttcatagtc gggaataata atacatctct
      301 tatagatgga gagttagtta ataacataat tactctatct atacctattc ctagtcctcc
      361 tgtaggaggt agacctacct ctaatgcttt taagaaatct tcatccattt caaaagcttc
      421 atcatcacct aattctcttt ttcttaattg gtctataaat cttccttttt ggtcaatagg
      481 gtcattaagt tcagaaaatg cattagctaa ctcccactta ttagcaaatg cctcaaatct
      541 atcagttctt cttgggtctt caacatttct cttagataat ggagatactt caactggatg
      601 atgagttata aatgttggtt ggattaattt atcttctcca aattcttcaa agaaagcatt
      661 tattacttct cctcttctca ttccaggttt tatctctata cctttttctc tagcaacttc
      721 tagtgcctct tcatctgtat ttatagtaga aaaatctaca ccagaatatt ctttaacaca
      781 atcttccata ctcattcttt tccaaggtgg tgtaaagttt atttctgttc cttgatagtt
      841 aactatcata cttcctgtag caacttcagc catatgtgca ataacatttt ctgttatttc
      901 catcatatca gtatagtctg catatgcttg atataattct attgcagtat attcaggatt
      961 atgttttaaa tccatacctt catttctaaa cattcttccc atttcataaa ctttatcaaa
     1021 tccaccaaca ataagtcttt ttaaatacaa ttcattcgct attcttaaat acattggtat
     1081 atgtaaagta ttatgattag ttataaatgg tctagcattt gccccacctg ctatagtatt
     1141 taatattgga gtctcaactt ctaaaaatcc tctatcatct aagtatgctc ttaatgcttt
     1201 taatgctttt gttctaatta aaaaagcatt ttttacttct ggatttacta ttaagtcaac
     1261 atatctttgt ctatatctta gttcttggtc ttttaatcca tgatattttt ctggtaaaac
     1321 ttgtaaagat ttacatagta atactacaga tttagccttt acagatattt cacctttttt
     1381 agttttaaag atttctcctt caattccaac tatatctcca atatcatatg ttgaaaatac
     1441 actatattct tcctctccta tagcatctag tcttacataa gattggattc ttccctcttg
     1501 gtcttgaata tctataaagc cagctttccc ttgtattctc ttgctcatta tacgtcctgc
     1561 tatttttgta gttttaccct caagagaatc aaagttatct tttatatcca tagaataatg
     1621 agttctatca tatctacttt ctttaaaagg atctctacct gattcttgta atttttttag
     1681 tttatctctt cttacttgaa gtacttcact aaggtcttct tgaatttgcg cttcttcatt
     1741 gctttgttga ttatttttca tttttttcct ccaaattatc ttcttatttc taatatttca
     1801 aacttagcaa ctccatcagg aacttgcact tccacaactt ctttcgcagc tctacctaaa
     1861 agtgctttcc ctacaggaga ttcgtttgat attttaccat catatgggtc tgcttctgtc
     1921 gaaccaacta tagtatattc aacctcttca tcaaattcta aatccttaac tttaactatt
     1981 gatcctattg taacaacatt aaggtctatt ttgctttcat ctatgataac agcttttcta
     2041 accatatttt ctaattttaa aattctttct tcaacttgtg cttgttcctt tttagcttca
     2101 tcatactcag cattttcaga taaatcacca aaagatatag caacttttat tctctctgca
     2161 acttcttttc tctttacaac ctttaagttt tctaattctt cttctaattt atcatatccc
     2221 tcttgtgtca ataaaaactc tttattttct tccattataa atacaactcc ttcacaaatt
     2281 tcttaggaat tttcctaatt atattttaaa aattttataa tcttttttta atttatgcgc
     2341 tatattataa gtcactatta tttttatgtc aaggagtctt ccatatagta ctccaatttc
     2401 gacacaactt cttgatagtc ttctattttg ttaatttcat cccttaattt tgcagagttt
     2461 ctaagtcctt ttaaatacca agcgatgtgt tttctcattt cccttacagc tacatactct
     2521 ccatgttctt ctactgcaag ttttaaatgc ttaatggcag tacttatttt ttcgttaaga
     2581 gttggctctg gcaaaatctc gcctgtattc atataatggt ttattctttt aaatatccaa
     2641 gggttacctt gagcacccct acctatcatt atagcatcac aaccagtttt gtctaacata
     2701 tttattgaat cttctattgt aaatacatca ccatttccta taacaggtat actcaaattt
     2761 ttcttaatct ctgcaattat atcccagtct gctttgcctg tataaaactg ttctctagtt
     2821 cttccatgta ttgctaaagc acttattcca caatcctctg ctatctttgc tatttcaaca
     2881 gcatttacac tattatcatc ccagcctttt cgaatcttta aagtcactgg cttttttgag
     2941 ttcttcacta ctgccctcaa tacctcttct gcaagcttag gatttttcat aagtgctgaa
     3001 ccatctccat ttttaactac ttttggagct ggacacccca tatttatatc gagaatctca
     3061 tttgaataat cattcattat ctctgctgct cttcccataa actctggttc tgaaccgaat
     3121 atctgaactg ctacaggatg ttcttcctct tctatcttaa gcattttttt agtattttca
     3181 tcatcatagc aaagtgcctt accatttatc atttctgtat ataaaagccc acatccttgt
     3241 tctttacaaa ttaatctaaa tggaaggtct gttacacctg ccataggtga tagaaaaact
     3301 ttatttttca attctaaatt tcctattttc attgtttttc tcctattttt gcctttattt
     3361 tgtgatttaa ctttaaattc ttatttcctc attataatat accacatata gctagaaatc
     3421 tatcataaaa agggaattgg atatataacc caattccctg tatattaatt attcttggtt
     3481 tttttcatat attattctca atccttctag tgttaaaaaa ccatctacat aatctatact
     3541 tttcgtctct gaagcaatca gtttagctaa cccacctgta gctataacct taacatcatc
     3601 acaattcaat tctttcttca tcatacttat tattttgtca actaaaccaa catatccata
     3661 aattattcca gattgcatgg ctgatacagt actcttacaa atagtcatac ctggcttagc
     3721 taattctact ctaggtaatt tagacgcact ttgaaataac gcctcactag atatttttat
     3781 tcctggtgct attgttccac ccaaatattc acctttttca gagatagcac aaaatgtagt
     3841 tgctgttcca aaatcaacaa gtatacttgg tgctccatac ttttctatcc ctgctacagc
     3901 attaactatt ctatctgccc caacttgttt tggattatca tattttatat ttagacctgt
     3961 ttttatgcct ggacctacta ttaatggctg ttttttacag tactttatac aaaagttttc
     4021 aagagaatgc ataacattcg gaactacaga tgatattata acatcatcaa tatcacttat
     4081 attcacatta tcataatcaa ataggttact tattaggatt ccatattcat cagacgtttt
     4141 ttctctatct gttttaattc tccagtaatt aactaatttg tcacctttat atatacctaa
     4201 aaccatatta gtatttccaa catcaaatac tagaagcata tgtcattaac cttctttcta
     4261 ttttttgctt ttcttaagta cagtggcaac agcagctact actaaagccc ctactattgc
     4321 ttctggcacc ccatttgctg cggcaagagc cattattact tttcctgctg aaccactttt
     4381 acccattgca tgaagatact tgtcagcata taagacatat atcattccta aaactccagc
     4441 tgtatttaac agagaaccta ttgcccctgt aattaaacct gatatgtata ccttatttgt
     4501 agccttaatt attaactgat atacataata agctaataca cctatcataa ctcttggtaa
     4561 tattgaaact aatggattta taaaaacaaa agatgttact gttgggtttg ttatagcatt
     4621 taagagactt gatatcccaa atataaatcc tattgctgcc ccaactattg gtccttccat
     4681 aatagctcca ataataactg gtatgtgcat tatagttgct tttgtaggtc caactggaat
     4741 aaatcccaat ggagtcatag atagcataat agaaatagct gatagtactc ctattatagt
     4801 catctttctt acatttactt ttcttactga tgcacttgtg ttcatcataa aaaatacacc
     4861 tccgttccag ctcattaaga tgccggacta cttaattttt taattttttt cgtttttttc
     4921 ttataactat gagtcaagct accattgtag gatgcccgct ctaacaagaa taattttatc
     4981 aagatgagtt gccattgtca acaatttgac aagaaaaata tgctaatttc atcatatttc
     5041 tcgttttttt actatatctt tatatgccat tatattttct acactatccg ctttttttac
     5101 tgcatttatt atactttttt ccacaacctc tacagccaat gaacctacta atgttatatc
     5161 tgcttctatt tctcctgttg atattgtgaa tattgtatct ccatcatgag gtgtatggat
     5221 aggaaaaata gacttagcat atccatcatg tcccatttga gatatctttt tacatccagc
     5281 tttggttaac ttagcatttg ttgcaattat cccaattgta gtattatcta cactaaatcc
     5341 acctttactt tcaccttgtt tcattaattt atatgtatca attgtttctg tctttttatc
     5401 atttaaagca ccagctataa ccttaccatt ttcataaaca tctccaagag catttacagc
     5461 aattaatgca gatacaatta gaccattatc taattttata gaatagctac ctattccgcc
     5521 tttcatagca tattcctgac ctcttatctt tccaacagtg gctccacaac ctgctccata
     5581 atttccttga tttaaaatat cagtatttgc aacttcacaa gctctataac ccatctttaa
     5641 atctggtcta catttaaagc tccctactcc taaatcaaat aaaactgctc ctacaactat
     5701 aggcacttta cctacaccta catcaaatcc aattcctttt tcttctaagt atctacttac
     5761 accacaagtg gattcaagac catatgcaga acctccagcc aaaactactg catgaacttt
     5821 ttgaaccata tttacagggt ctaataaatc ggtttctcta gtacctggag cagagcccct
     5881 tacatcaaca ccgcaagtag ctccttcttc acatattact actgtgcacc cagtaagacc
     5941 atttctatcc tcaacctgcc caacctttaa gccttctata tccaaaatat tattatacat
     6001 atcctttttc tcctcttata gaaacttcac ctgaaataat ttcttttata cttccatcac
     6061 ttctttgaac taataaattt ccctcttcat ttatgtctaa acatttcact agttctctat
     6121 tatctccttt taatacatat atatttttat ctataattgc agaataatct ctacatattt
     6181 ttaaggtttt ttctttggaa ttatttacag tataatctaa atataatttt tcaaattcag
     6241 tcaatacatt cctaacaata tctactctgg ataacatata cccttcttta tacaatgatg
     6301 tggctttgtc agctatttct tgttcaaaat ccattatttt aacatttatt ccaattccaa
     6361 gaacaacata atttgttctt tcaatttcag cagataactc tgttaatata ccacatattt
     6421 tttttccatt taaaataaca tcatttggcc attttattaa tgtatttgct cctagattgt
     6481 ttaaagcttt cgttacactt gctcctgcta ttaaagttat aaatggggct ttgaatggtt
     6541 ccatatctgg ttttaatata atactcatcc agattccttc atgaggtttt gaatcccatc
     6601 ttcttcccaa tcgtcctttc cccatatctt gatgttcact taaaatcaca gtcccatcta
     6661 cactatttat agctatcctt ttagcatgtt catttgttga atctatagtg tcaagatgtt
     6721 caatattttt tccaataaac tctgtatcta atttatagta aatattctgt gggcttaata
     6781 aatcagttgg attttccgct aatcgatacc cttttttatt tacagattct atattatatc
     6841 cctcttcttt taaggctttc atatgtttcc atatagctgc acgtgacacc cctaattgtt
     6901 ttgataattc ttctcctgaa ataaattcag atttattatt taatataatc tctattattt
     6961 tatctctcat atttcacctc taatatgaca taatttcatg acttattttc ttctaataaa
     7021 taattataca ttattaatat acttttaata ctattaattt taaatttcct ttttaattat
     7081 tataatggtt gttttatatt ttatattgat gatttatata attttacatt ttatttaaat
     7141 aattttaaaa taataaaaaa actgaaagta tcaattaaat taatacatgc aaatataatt
     7201 tgcttattaa tttaatatta tactttcagt tttagtattt aatccacatc agataataat
     7261 cttaaaatga ccaaggtctt aaaactattt atctttatca gatttatctt ctaaattttt
     7321 atttatatca ataacattgt ttctttcttc ttcatctttt tctaatttta tagttaattg
     7381 ttcttcaact tctttagaag aattttcctc tgtaactgca ttttcaattt cattcagagg
     7441 taactcttta ttaaatgctt ttacaaattg ttctgcatct aaagtttcat atataagtaa
     7501 agcttgtgct acatactcta atctatccat attttcttgt aatatacttc ttgtcttatc
     7561 atatgcacca tcaactatac gctttgtttc ttggtctatc tcaaaagcaa cttcttcaga
     7621 ataatttctc ttacttgaaa aactattacc taggaatact tcatcatcac tgtcaaatga
     7681 cattggacca agtttagaac tcattccata ctttgttacc atacttcttg cagtagctgt
     7741 aactctttct aagtcatttg aagcacctgt agatacatcc ttaagtacta gctcttctgc
     7801 tactctacca ccaagtaaaa ctacaatatt ttctttcatt tcattttttg tagcatagaa
     7861 cttatcttca acaggaagtt gcattgtaaa ccctccagct cttcctcttg gaactattgt
     7921 aacttgatga acaggactta cttcttcaag tacatgggca caaactgcat gccctccttc
     7981 atggtatgct gtaagtcttc tttctttctc acttattact ttcgattttt tagccacacc
     8041 tgctataacc tttgttatag cttcttctat agtttccatc ttaattttct tttctctttt
     8101 tctagctgtt aatattgcag cctcattcat taagttctct atatcagcag gagtaaatcc
     8161 tggtgttctt ctagccaaga catccatttt tacatcatca cttaatggtt tatttcttga
     8221 gtgaacttta aatatagcct ctctaccttt tacatctggt gtaccaacaa caacttgtct
     8281 gtcaaatctt cctggtctaa gtaaagctgg gtcaagtata tctggtctat ttgtagctgc
     8341 catgattatt ataccttgat ttaccccaaa tccatccatt tcaactagaa gttgattaag
     8401 agtttgctct ctttcatcat gacctccacc aagacctgca cctctttttc taccaactgc
     8461 atcaatttca tctatgaata tgatagctgg agcacttttc ttagcctgtt caaataaatc
     8521 tcttactctt gaagcaccaa ccccaacaaa catctcaacg aaatcagaac cacttatact
     8581 gaaaaaggga actcctgctt ctcctgcaac tgctcttgat agatatgttt taccagttcc
     8641 aggaggtcct accataagca ttccttttgg tattcttgca cccaattcaa tatatttctt
     8701 aggattcttt aagaaatcta ctacctcctg caaatcttct ttttcctcat ctagtccagc
     8761 tacatcttta aatgtaactc tagttttttc atcatcttta tgaaccttag cttttgattt
     8821 gccaaaactc ataacctttc cgcctccacc ttgagactga ttcataaaca cgaaccaaat
     8881 aatcaccatg aagaatatta acagtaatgt aggcaacatt tcgacaaacc aaggtgtaga
     8941 tggcttagct tcaccaccaa aagttagttt cccagcctta gcttggtcta acacctcatc
     9001 tgcaagttta tttcccatca cttctgttgg aacatatgac ttaaactttg tatttgtatc
     9061 ttttatagta ccttctacag aagtttgatt tacaaagtat aatcttgata tattctcatc
     9121 tgttaattct ctatatactt cagagaattt taaatctttt attttttctg taggcttacc
     9181 tgagaattga actataccta ctataattat aaaaacaagt agataaaaac ctgccccttt
     9241 tagcaactta ttcaatatag aagccctcct ttcatacatt gctttgccct ataatttgtt
     9301 aaaattaatt tttaatactt cttttgtgtt ttcatcaatt ttataatttt cactgatttt
     9361 ataatctcca acacatatta ttccttcact atctgtgatt ataggaattt tacatctttc
     9421 ttctcttggg atttttaaat ctatgaataa atctttcact tttttacttc ccatagcaag
     9481 ctttatctta tcaccttgcc ttctgcttct tattactata ccccctttta ccttattaaa
     9541 atcaaaccct ttagatgaat tgtctaattt catactctta tacctatcta tgctcattac
     9601 ttgggtctca ataatttgtt ttaattcttt tattttaata aacccattac taggtacatt
     9661 gtaataaaat tcaatttcct cattaacaat ttcctcattc gttaatatta tactgtcttt
     9721 ttttctataa acaaatatat ttcttggaag agttaacatt ttatttactt ttgaattatc
     9781 ttctaaagac attatatctt ctatatgtct ttggtcaaca aaattagtat ctcctagtat
     9841 atgttttatg gaatttctaa gaactctaac tttgatagca tcatgtaagc aaacaaaatc
     9901 gtctaaattt atctctacaa agcctttttc ttttatattt gaaacttctc taaatttagc
     9961 ttctgcctct ttctcaatat aatcgttatc actttttaaa ctattgctca tcctcactat
    10021 agattctatt acatttgaat taaaattatc cttcatatat ggtaaaaggt ctaaccttat
    10081 cttatttcta gtgtatatat tttctaaatt tgttttatct atcctcggat ttaaattata
    10141 agcttcacaa tactcctcta tctcatttct ttccacgtct aatatgggtc taattatgca
    10201 attatctcta atataatcaa ttccctttaa gccttttaaa cctgttcctc tcatgatacg
    10261 catcataacc gtttcggctt ggtcattgag attatgacct attgctattt tattggcttt
    10321 aatcttatcc ttaatttcat aaaacatttc atatcttagc ttccttgctc cttcttctaa
    10381 tgataatccc tcattttcac aatattttgg tacattaata gactttacaa agaatataat
    10441 tcccatatcc tcacaaagtt tagatacata taatgcatct ttttgagctt ctatccctct
    10501 tatttgatgg tttaaatgtg ctgcatatat ttctatatta aaatcttttt ttaatctatt
    10561 tagtacatgc aataaacaaa ctgaatcagg tcctccagaa agacctaaaa ctattttatc
    10621 gcctttttgt attaaattat gtttatttat agtacttagt accttatcaa atatcatatt
    10681 tatccctctt acacttagtt ttattcactt ttaagtatac tttctataaa aaaacgctaa
    10741 acccttgtat caattttaag aaattaaagt ttaatataaa tataataata ccaaataaat
    10801 taaataaaag gtagtttttt cactaccttt ataaaatttc tggaaaatag ccatagttat
    10861 tatttagtac ctatgtatat atctatttta ttcctaccta aaaataaatc ttgtaccttt
    10921 atattatagt ctattttcaa ttttatgtgt tccccttctt gtatatttat atctaattct
    10981 cttgtatcaa tatcaattaa taaagtacct tgtggggtca catactttgt agtactactt
    11041 tctccacatt taaacagcat tgttgagtta atagcaccaa actttttgat actaacttga
    11101 tttttaagta tctttatagt attagttatt tctatatctt cttctttttc cttgtaaata
    11161 acatatattc catcattttt ataaaaaatt ttaccaaagg cagtcatctc tatggtgtcc
    11221 atatttcctt tttcatcata ttgtctagta tttatactta tttttacact caacgatttt
    11281 tcctccatag attcttcata taaactagta tttttgtatt tctacttttt gaatcttatc
    11341 aatttttttc tttaagaact ctcttgctat atctgaaaat ttttcaggta tatctgatac
    11401 ataatattga taagttccat gaatttctgt attgtttata atattttgca cctctaaaat
    11461 cttttttaaa tcctttgcag tctctttagc tggattaaca agttttatat gctctccaac
    11521 ttcttcacct attgttctct ttaaaatagg ataatgagta catcctaaca ctaaagaatc
    11581 tatatttttt tctttcaatt cttgtaaata tatctttgct gtcagtttag ccacttctgt
    11641 atttgcccat ccttcttcaa caataggcac aaataaagga catgctttat taacaatttc
    11701 tatactctca tctattttag atatttccaa gttgtatgct tttgaactta tagttccttc
    11761 agttcctatt attcctacaa ttttgttttt tgtagaactt acagctgttc tagctcctgc
    11821 ttctataact ccaattatcg gtatatcata tttttcttga gcttctttta agcttcttgc
    11881 agtagcagtg ttacatgcta taactatagc ttttacacct tttgttttta aaaagtttat
    11941 agcttgaaaa gtatatttaa ttatagtttc tttggatctt gaaccatatg gtattctggc
    12001 agtatctcca aagtatatta tatcctcatt tggtaatatc ttcattatct cttttagaac
    12061 tgttaatcca cctaatcccg aatcaaaaac tcctattggt ttattgttca tctttataca
    12121 acacctactt tacatttttg tgtttaacaa caacttattt atcgctaaaa tttatattta
    12181 tattcataat taatatatta ttacctaaat gattattata gtttatttta cacaaaaaat
    12241 aaaggagcta tcttaaatta gaaaataatt tctacaaaaa gacaactcct ttcactactt
    12301 aaaaatttaa atttattttg caaacacatg atttcctatt ctcatataag gattcaatct
    12361 atttatccat ttgcaagtgg ctatttctgg attccaaaaa tatactgctt tattagttgg
    12421 gtcagttcca taaagagctt cctgagctgc tttacgtgct gagtctgttg gtgccatctt
    12481 tattgaacca tcaagcacaa ctgagaaagc atttttttga tatatgactt cttctattgt
    12541 gtctggaaat ctagggtcca atactctatt tattactaca gctgctacag caacctgtcc
    12601 ctcatagctt tctcctctag cttctcctgc aaccaactta gataataaca tcaagtcatt
    12661 atcagataaa tttattactt ctttctttgc tatatttttt tcttgtttgt ttactttctt
    12721 aatattttct gttggttcat atgcaaatac ctctttaata ggcataaata taaaagtcaa
    12781 agctaatagt gtagccatca gtttactcca cctgtccatg attaaaacca ctccttacat
    12841 tcttattttt aaaatttttt ctctaaatcc ttgactactt caagtacttt aaatttaatt
    12901 tttactctac ttgttttttt tggtgtatct tgttttatta acttatattc tttatcagtt
    12961 aaaacttcct tcaacttatc atctgtagat ttatctatca ctccattaga ttcatcaaca
    13021 aaacatagtg gagggaacat aacacaccac cagttttttc cttcaccttt cccaatgata
    13081 atctttaatg ctttatattc accagctgga agaactatat tagaatattg tttagtaggg
    13141 aaattgctat attgtatacc tacctttaca ctgtaattat atccattttt aagaataata
    13201 tttttactta tttcttccaa actactatat tcctttttta taatagcttc actttctttt
    13261 atactttttg aattttgaag ctttggctgt aaatatttta ttacttcatc tctaactttt
    13321 agtttaagtt cttggtcttc atctgtattg ctgtttgcta taacatgaaa tcttattaat
    13381 ttatctttat aatcttttga tatattgtct acttttttta cctcaccatt tattactatt
    13441 gtcattattg atatgactga tataagactt aatattaata gtcccaatct aatcttaatt
    13501 tttctcattg aaaattcccc ctcaagataa atttatatca tcaattatgt ccaattaaat
    13561 ttaatttatt cataatattt tttaatacta ataaaaaaag ctatcttaaa atgattacaa
    13621 tcattttaaa atagcttttt aaaccttata ttttataagt ctttactaat catagcctta
    13681 tctatcataa actgctctgc tttttctata tgctctgtag catacttctt ggatagctca
    13741 ctatcacctt taattatagc atcaagtaaa tatttatgct cttcaacaat attatgtgat
    13801 gtatcataag cagacatata ggtaactcta aatctatata cttgttccca caatgagttt
    13861 attaattgga ttaatttttt attattagtt gctttaaata tacattcatg aaattctaca
    13921 tcaagtttta ataacgcatc tacatcggat tccaaagtgc tttttttaaa ttcttctgat
    13981 atatctttaa gctttttaat atcttcatca ctaattcttt cagcggcaag atatgctgct
    14041 aagccttcta ggcttgctct tacttctaat acatccataa tatcctttaa tgacatattt
    14101 gccacatacg caccttttct aggcaacata acgactaaac cttctaattc aagctttctt
    14161 attgcctctc tcactggagt cctacttact cctaattgtt ctgctagttg cacttccatt
    14221 agcctttgac caggctttaa ttttccttct aagattgctt ctcttaaatt ctcaaagact
    14281 acatctctta atggcttgta attatccaag ttcagtttag ttaaattctc cacaaatttc
    14341 aacccctttt tgactgctat taacaacata cacctgttta tattttttta ataattcttt
    14401 ttttccaatt agagcatctt ctttattttt aaatagtcca aaaactgtag gaccacttcc
    14461 actcatcata gaacctaaag cattattttt catcattact tgttttatat cacttatttc
    14521 tttgtgttta cctatagtaa cattttcaag aatatttacc atgctttctg atacagcttt
    14581 tatatcttca gattttaaac actctatcaa atatttatta tttggtcttt ttttaatatt
    14641 attcaaatct aatccttgat aaacttcttt agtagaaacg aataagtctg gtttgcatat
    14701 caatatattt gtatcacatg gtaaaccttt gatgtttgtt agcttttctc caataccttg
    14761 agctaaggca ggtcttcctg atatacaaaa aggaacatct gcgcccaaat taagtcctat
    14821 ttcttttaat tcatcttcag ataatctaag ttcccatagg tgatttaaac ctactaatac
    14881 tgcggctgca ttagaacttc ctccagccat ccctgctgct acaggaatat ttttttctat
    14941 gaatatttct acaccttttt ttatataaaa tttattttta agtattttgg cagctttata
    15001 aacaatatta tcttcatcta aaggaatatc taaactagtg ctttttacct ttatttcatc
    15061 ttcgtctaat tcttttatct ttactatatc gtataaatct atcgtttgca ttatcatttc
    15121 aacaaaatga taaccgtctt gcctttttcc caagacatca atggataaat ttatttttgc
    15181 tctactcttt agtcttataa aatccattct atcctccttg tttaaagaat aatgcatcac
    15241 taacaataat attatactac aaaaaataaa ataatagggt attataatac cctattatac
    15301 ttaattactt taaatttttt aatattaatc tactaatacg actttttttt ctaaaataag
    15361 acacttacca ggcttaattg gttcatcaag ttttatatca ttaagttctg caatttcatt
    15421 ttctgttgta ttgtattttt ttgctatatt ccagaaagta tctccttctt tgcatatata
    15481 tacgattatg ctaggagctt tagataaatc atatacacct tggtcttctc ccttgataat
    15541 aaaattttct gctttcttat ctaatgcttc agtaaatctc ttaattttga taatcaagtc
    15601 tatctggtcc ctattcaaat caacttcgac tttatcaata catgctgtat taaacacaga
    15661 tgctgtatca gttagattat ccatttctat atcatgttca aatggaattt cctcactaat
    15721 tttataaacc aattttaaac cttcaactgg gacaaataaa atttcaactt ttatgattcc
    15781 ttgtataatg cttttatttc cttcaatata actattttct atagacatgg ttggacaaac
    15841 gcttacaata tcttttattt gtatatcatc attatcattt cttatgcctt ctctaaccat
    15901 aaatgtttct tcactacgtc ttaaagtctt atttaattga ataggtttgt ggtcaaattt
    15961 tattatcttt tgaggcgaat atgcatcttg taatacttct cttgtaactt catctgtaac
    16021 ttttacctta caacaagcca tacaatctat ttctaaaaga cctgtgttgc tttcactatt
    16081 ttgcttgaaa atatgattaa aatctgacat agacaacaat acttcttcag tcataccatc
    16141 actaactcca gggacttcta caaattgagt aaactcaata cctactctat ccaactctac
    16201 aagttcaccc tcatatgtac aagctaacgg atttatttct aatactccac caataataac
    16261 tttattgtca gtcactctac tttcttttac tttaacacat ggatttaaac ttattataga
    16321 ttgaatttcc tctgtgttta ttgttatagt atctcttata gaactttctg ctttttctat
    16381 tccaactata tcttgaaaac aaatttcctt tctgtgcttt tgtatgcctt ccacttgcgc
    16441 cacatctttt acaatgtcta acctttgctt ttcaaacaaa cttcccctta tattcataag
    16501 agctcctact ttaatctttc tctcattcat tatagtacaa tccatatgct ctacttctga
    16561 aaataacatg tattccatat cttgaacaac attgtctttt tctattactt cgtttatatc
    16621 tacttttccg tctacattag aaatagtatt tttatcatct gcaatataga ttacattata
    16681 attaaaacta cctctgcaaa gtatcttccc atctgccatt tcaatttttt taagtgatat
    16741 gtatccttct gtttttacaa tttcatatac atctaatttt ttgtctggta caactgcctc
    16801 cgcttcaatg aatgtttgaa attttccaaa gtctattcta ttgtcaactt taattacatc
    16861 tttaattaat tccatagtct acctccttaa gtattataaa aatatcacac tatatttata
    16921 tttaaggagc aaaaaaaaaa ttacacctat ttttaaatag gtataatttt aacttatttg
    16981 taatttatct tgatctctaa aaacttgcaa ctttacatta gatgttaata aatctgagta
    17041 actataagat actctaggat atccattact ttcatcatct aactttataa caaacacgct
    17101 tggataaact ttttctagta taccctcttt tgtaataatc tgctttcttc ctttattagc
    17161 tttcaacaat attttctttc ctatatgtct ctctaagctt actcttatct tatctagagt
    17221 ttgaacagta gccacaatat caccttcttt aacgtttata tgtttacata atatcacata
    17281 taaacatttt tgtcaaatgt ttaaactaat tattttatat attttttgca aaaatgtcaa
    17341 tactattttt gaagatatac agtataatta tgttaatttt tgctaatgta atattgtttt
    17401 tggcattgcc catctaaatt ttcctctggt ttcagaacca ccaattccct taacacctgt
    17461 aatagtctgc tctataacct catcaatagg aactactaca tcaattcttg aacaagcaat
    17521 tttctccact ataaaaacag ggtctaatgc atgtatcatt atccttccag gcgaacttgc
    17581 atagtttgct cctgcactta ttattttttc ataatttgat tggcatgctc ctgcgaatat
    17641 gacaagatta tccaaatttg gttgccattt acgtgcttct tttactgttt taacaaaatt
    17701 tgaagagttt ctatagttac tcatatcttc tatatctcct ctttttattg tcattgcatc
    17761 atgacctgtt attactagta tatccggatt atatttttct agtagtgctc ttacttcttt
    17821 atactgattt tgctctgaaa cagctacacc tactgcaggt attccaagtt gagcatatac
    17881 atccaagcaa atttttaaat attctttatc tccatctatc tgtagtactt ttccaggcat
    17941 tccatatgtg ttaggattta attgaagttt tggaactgct ctagtcattc ttttttgtct
    18001 ttctttagct tttcttactg acttatataa taaattttct acatttttat ctattaatat
    18061 atcttttata tcaggtgctt ttactaattc taggtcatca atataagcat ctgctattac
    18121 tctaaatgca attcccttta gtatagctat tttctcatta ttttcatcta caccaaaaga
    18181 gactatttta aatactatat ctttgttatg tgactttcta gctacaatgt ctcctacctt
    18241 catagttaaa cctccaataa atttttatct tagtttatac tatgttcgat ataaaacttt
    18301 gtttcctaat gtcaaaaaaa catttcttat aatacttctt ttatcactct taaagcattt
    18361 ttatagtata tcttttctat atcatcttca ctaaaccctt ctttttttag aggttcatac
    18421 agtttaccca tctgagatat atcttcaatc tcaacttttg aatctattcc atcaaaatct
    18481 gaccctagag atacaacatc tattcctcca acatttacaa tatgttttat gtgtctaacc
    18541 atatcttcaa gtttactttc acttttatca cttaaaaata agtggaagaa gtttatacct
    18601 gtaactccac ctttatttgc taatacttta atcatatcat cagttaaatt tctagaatga
    18661 ttcatcattg ctcttgaatt agaatgtgta gcaattattg gtttagaaga aatctttgca
    18721 atttcataaa atccaccatc tgaaatatga gaaacatcaa ccaacattcc caattcattc
    18781 atcttatgta ccacttctct tccaaaatca gtaagccctt tttctctata ttctacttta
    18841 ttatgaggaa agctcaattc attcacatga ttccaactta ttgtcatcat tctaacccct
    18901 aagtcgtaaa atttctttaa attttctaat ttaccttcaa gtactgcacc ttcctcaata
    18961 gataaaagag ctgtaagttt accttctgat tggtttttca taatttcttc atagttagta
    19021 gctaaagcta tttcatcact attttttttc atttcctcat gaaatttatt tgccatactc
    19081 atgcaataat caaaaggatg ttttacttct tcagtatcaa caaaaagagc aaatgtttga
    19141 gctaatgaat cccccttttt caatctgtct atatcaacag aatatttatt agacttaagt
    19201 tcacttgttt ctacattttc cattaatttt gctatagtat cacaatgtaa atcaataaat
    19261 ttcatattta taccccctgt atattttata tttatatcta aaaatagaaa tagttgtcct
    19321 tatagacaac tattgacaac tattttttaa tattcttttt aatattactt aatcttattc
    19381 ttcttttaag ttaggtatta cttttttaac ttcttcaatt tgatgccatg ttttatctat
    19441 attattttca ctaatatatt tttttaaatc agttaaagat gtaaaatctt taactgataa
    19501 attgtcatca tcatctgcaa atcttaaaat aaacatgaga ctcccttact ttccacaaca
    19561 tttcttatat ttttttccgc ttccacatgg gcatgaatca tttcttccta tttttgcttc
    19621 tttaactatt gttttagact tcttataagc ctttattatt tcttttcttt ggtctgctgg
    19681 tagtatatct tcccaacctt gtaaattata tagccaatga gcctcaacag ttaccatatt
    19741 gttatataat ttttcccaat ctatttttat agaaacttct ttatcagctt ctacttcttc
    19801 taaaactata tcttctttta agctttcact tattccatct atgaatccca tgaaaaattc
    19861 attagtagtt ccaaattttt cagccaattc acttacttta ccttcaacaa catccatttt
    19921 attatttaat atttcattgt atattccagc ttcaactttc aaatattctt cccagaattt
    19981 tacttctgct tcttgacttt catgattatc acttaaattt ctccattcag tatataaact
    20041 catacttttt cctcctaagt atacctatat ttttatagtt tttatagtat aaaatctttt
    20101 agttagattg gaagtttctt ctttttgatt ataccatatt ctatttaaag ttaactctta
    20161 tattatatca taattaaaca attcttttaa tacttttata gcatactctt ggtttataaa
    20221 ataagggctt gtcgcaattg ttattacttt ggctttttta attaagtttt ttatcttgta
    20281 aaatttgagt tcaaatggta tatagtccat atcacttgaa aatatatcca aatctatgtc
    20341 aagaacaaac tctgattcta cttctaaatc aaatccatat agactatcta ttattattaa
    20401 ttctgaaaat atgttatatt ttaaagcagg ttttataaaa cttcccacat ttaaaacttc
    20461 atttgtatat ctaaatacat cttttagatt atttacatct acatcatagt tctcaggttc
    20521 tctagtatcc ttatgttggt ctacatgcac taacttgcat ccttttgtaa actcaccctt
    20581 taacaaagac tttgcccaaa aataaaatgc atggttatga ttgtcaaaca catatatctt
    20641 tttatttccc aggacatatt cgaccatatt ctccaatcct tttgctttta tttccttatt
    20701 ttcatctact tcattaaata ctacctcatt gcctaacctt acatcattta atgtaccatt
    20761 tataagtttt ggaacgtata tgctcttatt ttctctttta tcatatgaaa atacattatt
    20821 tcctactggt ttttctatat aaaatccact atattcataa ttttccataa aactttattt
    20881 cctccattgt tatataaata tataagttat aattttaaaa attaaaatgc acttattgta
    20941 aatcatatca tctattatag aaaacatctt atataactaa atataatatc ttctctcaat
    21001 atgtattata caataagtgt aaaatatatt tatataaaat atactacatt cctataacaa
    21061 aaccatcagc ttcaattgga agataactaa ctcttctatt caactccacc tttccaaaaa
    21121 atactaccca atatggtcta aaaatacttt caattttaac tatttcaatc tctgggattc
    21181 ctcccataaa cctatgagtt aatctcatag ccattttatt tgcctttgct tttactcttc
    21241 gttcgtcctt atcactatac tgcacaatat tacaatccac attttttaca acttcatatg
    21301 taggaaaatc ttgtactata ctaccagaac ctgtagaacc attcactaaa atttttaatt
    21361 catttatatc attagaatat ctcctattct tgaaaatgga tttcgttttg tgaattttaa
    21421 gtgtgataat tttaaactct atataatgaa gtctaacttc atgagcatat ttgttcttta
    21481 atattagctt actaaataaa gaattttttc cacttttagc tatctctata gcttcttctt
    21541 ttgtaattct tataggtaac gctcttatat ccataaaatc attctctttc ttattttttt
    21601 cttgtcattt ttaaaactct atatatataa tcaaaaaata ttacaattac aactgacata
    21661 actccatatg gaattatttt tccaactata atatcatttc tagtcaatat accataaaat
    21721 ataaatcctg ttattaaaaa gtacaataat actagaaaac cgtctattcc aagccatttt
    21781 atttctaaat ctttttttac cttagacact aatttagaca tagcagtttc tccttttata
    21841 gattattttg tgtatttaaa ttttcaccta ttctattacc aatcatccat gcaataaatg
    21901 aaaaaggtat agctatcaca agaggctcta actcaaagct ctgaactgga aatattttaa
    21961 aataaccaaa agtcactcca ccaacaatca ttcctaaaat accacccata gatgttcttc
    22021 ttccatttcc aagaagccca cctactatag gtacaaatgc cgaacccatc caaatacttc
    22081 ctacaaatat acatgcatca tatacatttt caaatctaaa tgctacgcta agccccataa
    22141 ctgctgctaa acatacacct attctagtaa actttactaa ttgtttctga gataactctt
    22201 tttttgtaat tcttccatat atatcatttg ctaatgtagc cccagctgct agataatagc
    22261 tatccaatgt tgatattata gaacctaaaa ttcctattat aaatattact cttattcctt
    22321 gtggtaatgt atctaaaact aaagttatat atccagcagc tggagccatt tctggataag
    22381 tagctcttac tataagtcct gtcatagtta acaccatgtc aaaacatatc catagtgaaa
    22441 aacaggttaa taaggctctt cttccaacct taggagaatc tgctgctgaa aatctttgat
    22501 aaaaagttgg gtctgaataa gctccaaaag aatatacaat taacatcaat gctttcatag
    22561 ctgacatacc ctttgtaggg tgcataagct gtggtgcatc tttgagtgct tctgtaagac
    22621 cttgaaaccc tcctatttgt tcaaattgac ctggcataac catagttaaa ccaaatacca
    22681 tacaaaaaaa caataccata tctgtaatgg caactcccat aagcccacca agtactgtaa
    22741 gtacaacgac tactcctacc attattaatc ctattaattc tttatctatt ttccatgcta
    22801 cgttacctat agttcccata gcagttattt ctccaagttg tgcacaatat ataaacatca
    22861 aaattgcccc aattatagca acatgcttgc cataaaaact ttccagtaaa tcaggaactg
    22921 ttatattggt tcttatatga atccttggac caacccacaa tgctaaaggc attctcccaa
    22981 tatgtgcagc tattgaccaa actgcccatg cagatattcc atatatagcc gcatactcta
    23041 cactacctac tgtaccaact cctccatacc atgatgcaac caaagttcct actattaagg
    23101 accatggtaa acttcgattt cctaggtaat aactttccat atcttttaca tttctagaaa
    23161 aataataacc tattcctaat aatataaatg caaatataac tataatccca ttttcccaaa
    23221 atgttaacat atctaatctc cacattctta tattttaatt tttaacttta atatctaaat
    23281 atgaacaact ctacccattt ccataaaaat aggtagagtt atataataat tataagtatt
    23341 taagtaatct tgttattaag caattttatt gtttaaacct ttattttctc ttttatttat
    23401 tttatttaaa gcataagtag caaatatccc tataactgca catactacag ataaaataaa
    23461 tatatatgta tatcctgttt gtccatattt atctaaccaa ttacctgcaa tagtgtaata
    23521 aaacgcatct ggagaaaatc ctataaatga agcaaatcca actactgctc ctgtagtttc
    23581 taaagatata ttagactctg aaacagatgc aaagtatata cctctaaatc caaataaaat
    23641 tacacttagt attatcatgt ttattacagc tatccatata aatcctgtac tcttaggaat
    23701 aaccaagtaa gcagctgtac ttactgtcat taatataaat cctacaaaca ttactcttat
    23761 agatgaacca actttatctg ttataacacc agcaactgga gatgctccca tttttataac
    23821 ataagttctt attattgata aagcagaaac tagagtcatt ggcattacat aaacttctga
    23881 taagtatgga cttaaataag ttaaacttga gaatactaaa taagttgaaa atattataag
    23941 accaattagc caagcctttg gcattgtaac tacttttaac aatgacttaa tacttaatcc
    24001 ttcatctgat gctccactat cttttttctc ttccactaag aaaaatagaa gtataccaca
    24061 tattatagat gctgcagaat ataaccatac tacatatttg aatcctactg ttatttcagc
    24121 aaatttacca aagaagtaaa gacctataaa agaaattaat gtacctgata cacctgataa
    24181 cccttcataa aaaccaaata ttcttccttg ctctgactca cttccttgca ttctaacaac
    24241 cttaacagag gctgagaaga aagttaatat tgttgtaaat ccccataata caaatattaa
    24301 aagtatcata ctatatgatg ggaatgtaga gaaataaaat ccaagtattc ctgaagcaat
    24361 taaagaaaaa gttattaatt ttctaactga gaatctatct gcaaaccatc ctcctataaa
    24421 ataagatacc attcctacta atccatataa actcaataag ttccctattt gtgtatgact
    24481 taatgctaaa gcttgttgca ttgggtcata aaatgatgat ttcatatatg gtaaactata
    24541 catagctgtt gttccaaatg ccaatatgaa tattatcaaa aatcttttaa cattattttt
    24601 gctttccatt gattctctct ccttaaaatt ttattgttta taatctcatt catataattt
    24661 tattttaatt tttctattgc ttgtttaaaa tcatttatta tatcatttga agattctaat
    24721 cctacagcta ttcttattag attttcccca ttcatttgaa ctaatgtatg ctcttcccct
    24781 aagcttgttg ctattgttgc aagctctaaa gaattcatta atcttctact agcttggtct
    24841 cttgtcaatc cattgatacc atccttaagt ttaaatgaaa ctacacctcc acctaaacca
    24901 ttcatttgag ttttacaaag ctcatattga ggatgagatt ttaatgctgg atactttacc
    24961 aattccacat tctcttcttc ctctaaaaat tctgctactg ctaatccatt ttcacaatgc
    25021 tttttcatcc tcatatctaa cgttttcata ccccttaaaa tcatccatgc actcattggt
    25081 ggtaaagatg ctcctgtaaa acaacataaa cttggccatc taactaaatc tattaattct
    25141 tttgaacctg ctactgctcc ccctaaagta tccccatgtc catttataaa cttagtaaga
    25201 ctatgtatta ctaaatctgc acctaaatct attggtcttt gtacaattgg tgtcccaaaa
    25261 gtactatcaa ctatagctaa gcttttattt tcatgagcaa tttctgctat tgtttttata
    25321 tctactaagt ctaatgatgg atttaaagga ctttctatat aaactatttt agtatttggt
    25381 ctcattgctt cttttacatt ttgtggattt actgcatcta caaatgtaac ttcaactcca
    25441 aatttagata acaactcaga catgaataat ttagtgtgtg aaaatattcc atctgaacta
    25501 actatatggt caccactttt taccaatgct aatatagtag atgttattgc ccccatccct
    25561 gatgtactag atatggcact ttctgcattt tcaagcatag ccaacttagc ttctaactct
    25621 gtaagagtag gattacactc tcttgtatac acatatcccc atgtcataca caactcttca
    25681 aaatgctcta ctgtctttgc agcaaatgtt gctgtttgac atattggtgt agccaatgca
    25741 cctgttgttg ggtctgcact atgtccccaa tgtattattt ttgtctctaa atcttgatta
    25801 atattgttac tcattttgat ttcctcccat tttgacttat ttatacaacg ttttccctac
    25861 caaatttcat caatttagct tagtcttaat acattttttc aagtatactt ttttatttgt
    25921 tataatttct aaaaatatga cacttatttt gtttttttac agttctaatt ataaactcta
    25981 tagtaactat agagtcaata tgtttttgat atttttttta caatgtttaa aatagtcagt
    26041 acaaaaatag gtatatctta gaataactaa agtcatccta agatatacct atttttatat
    26101 cacaatataa ataattacat ataagcatta gtttttttat cataaataga tttttc
//
LOCUS       DACULQ010000036        25656 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.036, whole genome shotgun sequence.
ACCESSION   DACULQ010000036 DACULQ010000000
VERSION     DACULQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.036"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(253..396)
                     /locus_tag="JA154_06110"
     CDS             complement(253..396)
                     /locus_tag="JA154_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine peptide SCIFF"
                     /protein_id="HAT7995436.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(462..842)
                     /locus_tag="JA154_06115"
     CDS             complement(462..842)
                     /locus_tag="JA154_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HAT7995437.1"
                     /translation="MRGGFFMGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDN
                     ITMVSSFITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEI
                     GMIYKLLLISAAGGIGGVLGVNFK"
     gene            complement(882..1175)
                     /locus_tag="JA154_06120"
     CDS             complement(882..1175)
                     /locus_tag="JA154_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAT7995438.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1309..2430)
                     /locus_tag="JA154_06125"
     CDS             complement(1309..2430)
                     /locus_tag="JA154_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAT7995439.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2509..2994)
                     /locus_tag="JA154_06130"
     CDS             complement(2509..2994)
                     /locus_tag="JA154_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995440.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(3006..4031)
                     /locus_tag="JA154_06135"
     CDS             complement(3006..4031)
                     /locus_tag="JA154_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAT7995441.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEYGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4349..5368)
                     /locus_tag="JA154_06140"
     CDS             complement(4349..5368)
                     /locus_tag="JA154_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAT7995442.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5475..6077)
                     /locus_tag="JA154_06145"
     CDS             complement(5475..6077)
                     /locus_tag="JA154_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAT7995443.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(6070..6579)
                     /locus_tag="JA154_06150"
     CDS             complement(6070..6579)
                     /locus_tag="JA154_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAT7995444.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6922..7551)
                     /locus_tag="JA154_06155"
     CDS             complement(6922..7551)
                     /locus_tag="JA154_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995445.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7754..8077
                     /locus_tag="JA154_06160"
     CDS             7754..8077
                     /locus_tag="JA154_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995446.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8345..9199
                     /locus_tag="JA154_06165"
     CDS             8345..9199
                     /locus_tag="JA154_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995447.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHTIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9385..10821)
                     /locus_tag="JA154_06170"
     CDS             complement(9385..10821)
                     /locus_tag="JA154_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAT7995448.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSEKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKILGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10892..12253)
                     /locus_tag="JA154_06175"
     CDS             complement(10892..12253)
                     /locus_tag="JA154_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HAT7995449.1"
                     /translation="MIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMVRMPINEI
                     STTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNSALAVTTA
                     GFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVIPTPGPLA
                     VAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKNLEQGKDS
                     IKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFIGDKNIAI
                     LLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASGIGNFLVD
                     TMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVLVGLAICA
                     GGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSVINGIIPL
                     PGL"
     gene            complement(12410..13300)
                     /gene="garR"
                     /locus_tag="JA154_06180"
     CDS             complement(12410..13300)
                     /gene="garR"
                     /locus_tag="JA154_06180"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089326.1"
                     /note="catalyzes the reduction of tartronate semialdehyde
                     to glycerate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAT7995450.1"
                     /translation="MKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDELISCGA
                     KYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTPTESIL
                     CANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSSSILIG
                     DNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTILDAKI
                     PMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGHLEDDH
                     SGIAQYFEKLAGVEIKKHNN"
     gene            13711..14382
                     /locus_tag="JA154_06185"
     CDS             13711..14382
                     /locus_tag="JA154_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7995451.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            14494..15033
                     /locus_tag="JA154_06190"
     CDS             14494..15033
                     /locus_tag="JA154_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995452.1"
                     /translation="MNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEEFGE
                     DKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEELHC
                     PYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCERIY
                     FHDDKVSEGKYTYGNKNCK"
     gene            15295..15603
                     /locus_tag="JA154_06195"
     CDS             15295..15603
                     /locus_tag="JA154_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995453.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(15765..17555)
                     /locus_tag="JA154_06200"
     CDS             complement(15765..17555)
                     /locus_tag="JA154_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7995454.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(17555..19282)
                     /locus_tag="JA154_06205"
     CDS             complement(17555..19282)
                     /locus_tag="JA154_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7995455.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(19527..19694)
                     /locus_tag="JA154_06210"
     CDS             complement(19527..19694)
                     /locus_tag="JA154_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995456.1"
                     /translation="MKDNLIDDYYIYILPIILGRGISLFKSGIDKTNLNFKKASNIGE
                     LVKLEYSKKQK"
     gene            20091..21203
                     /locus_tag="JA154_06215"
     CDS             20091..21203
                     /locus_tag="JA154_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid racemase"
                     /protein_id="HAT7995457.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(21567..22076)
                     /locus_tag="JA154_06220"
     CDS             complement(21567..22076)
                     /locus_tag="JA154_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HAT7995458.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVLVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(22078..22725)
                     /locus_tag="JA154_06225"
     CDS             complement(22078..22725)
                     /locus_tag="JA154_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995459.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(22739..23911)
                     /locus_tag="JA154_06230"
     CDS             complement(22739..23911)
                     /locus_tag="JA154_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HAT7995460.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPAICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            24179..25555
                     /locus_tag="JA154_06235"
     CDS             24179..25555
                     /locus_tag="JA154_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995461.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILSNYMNITLRSANRILSILYKAGIATIVNTKLDNQRGKPKKIYKVDFLSFL
                     NKMQKLNS"
BASE COUNT         8647 a         4359 c         3021 g         9629 t
ORIGIN      
        1 agagtttaaa attaaaaatt attctaatac atgtaattga taaaaaataa aatataagag
       61 tagctacttt aaaataaatg ctcttacatg attagatatt tttgcataat taaacgataa
      121 gaaatcaaat ttacttctac ataaatagaa tatatatttt tatataataa ttgaaaataa
      181 aagaggatac ctcataatga taaaagaatc actttgagat accctcttac aaacaactaa
      241 atcaataatt ttctatcttt cacactcttg atttgcaact gtacaagaag ttttacaagc
      301 tgattgacaa gaagtttgac actctccaca tccacctttt gcagcacttt gctttaaagt
      361 agcttgagat aaagttttta catgtttatt ttccattttc aacgcctcca tttctaattg
      421 tttcctattg attatatcat aaaactactt taatataaac tctatttaaa gttgacaccc
      481 aatacgcctc caatgcctcc tgctgctgat attagcaata acttataaat catccctatt
      541 tcaaatacaa acttttcttg tgctaaaaac acagtcaaaa aaatacatac tatgtacaaa
      601 agtcctacta aaagcccata cataagtcct ttttctttca tgtgttttga agtgtaaaac
      661 cctcctatag ctgcagaaat agtagttata aatgatgaga ccatagttat attatctcca
      721 ccaatatctg taaatgtcaa aaataagtta tacactaata aaacagccaa agttattata
      781 tatgcatatc ctagaccttt aaaaatataa ttagattttc ccataaaaaa acctcctctc
      841 aaattaatat atattcgaga aaaggctttt aaattactaa tttagtcttc ttctatttct
      901 tcttttttag atttaacagt tcctatagcc catttttcaa atggaacttt agttctattt
      961 ggtcctaatt ctaatattac aacatcatct tcaactttag ctacattagc tactattcca
     1021 cctattgtaa tgaccttgtc tcctacactt aggctgcttc tcatttcttt taattgtttg
     1081 tcttttttct tttgaggtct aatcattagg aaataaaaga ttgcgattac tactacccat
     1141 agacctatac tcataattat ttgttgctgt ggcattaatt tttcactcct ttgtaaatta
     1201 tactcttttg taaatcataa taaatgttta tataacaatt tactttctct atctttgagt
     1261 actattgaat attatatatt aaaatgatta taattaatag tgcaaaacct atatttcata
     1321 tccatatttt gcaaaaaatt catttctaaa atcaagtaat ctatcttcca ttatcgcttg
     1381 tcttatttgt ttcattaaat ttaataaaaa gtgaagatta tgagtagtta ttaatcttgc
     1441 acccagtatt tcatttgctt ttataaggtg tcttatataa gctcttgaat agtttttaca
     1501 agcataacaa tcacattcag ggtcaagtgg agtaaagtct tcagcataag ttgcatttct
     1561 aactactact ttaccttggc tagtcattgc agttccattt ctagcgatac gagtaggtag
     1621 aacacaatca aacatgtcta ctcctcttat aacaccttct actaagtcat ctggactacc
     1681 aactcccatt aggtatcttg gtttatcctt tggcataagt ggagttgtat gctctaacac
     1741 gtcatacata agtggttttg gttctccaac actaagtcca ccaatagcat atcctggtaa
     1801 atcaatatta gttatttctt tcgctgattg ttcccttaag tctttataca tcccaccttg
     1861 tattatacca aataaagctt gcttgtcggt attattatgg gcatctttac atctttttaa
     1921 ccatcttgtt gttctttcta aagaattttt tacatattct ctatctgatg ggtatggtgc
     1981 acactcatca aatgccatca ttatgtctga acccaatgca ttttgtattt ccatagcttt
     2041 ttctggagtt agaaaatgtt ttgaaccatc taagtgtgac ctaaattcta caccttcttc
     2101 ttttatcttt cttaaaggcc ctagactaaa tacctggaac cctccactat ctgtaagtat
     2161 tggtttatcc caattcataa acttatgaag accaccagct ctttttataa gttcatgacc
     2221 tggtctcata tataaatggt atgtgttact aagtattatt tgtgaaccaa tctcttttaa
     2281 ttcttctggt gtcatagatt ttacagttgc ttgagttcca actggcatga atattggtgt
     2341 ttcaataatc ccatgtggag tgtgtaatct tcctaacctt gcaccactct gcttacaagt
     2401 ctttatcaat tcgtatctta ctgcgtacat aaagttctcc ttaatcatat ttatttctca
     2461 ttatttctta agatatattt gtaataagct attcatataa tttattgtct aaatagattt
     2521 atcttcgtct tctttgttct gttgttcctt aattgcttct tcatcatgaa tactcattat
     2581 attaagagca ctcttgtctt ttgttactaa aaactttttt atagcaaata gtcctgctat
     2641 agctataact cctattaaaa catctatcat accttcattt ttaggcagta gcaacatttt
     2701 tctagcaatt gcgtaaagta atgcctcaat aaatgctcct ggtatatgaa gagatagcat
     2761 tactacaagt tcaacaccta ttactaaaag taatatttgt gctaaaaaat cattaagctg
     2821 actgtattgt actggatttt gaaaatcaac tatatatgct ccccacataa gtcttagaac
     2881 atctatagtt cctaaaaata ctgctattag aactactact gctaaaacac gttcaaatat
     2941 aaaagcaatc tttaatatag ggttattctt tttttgtttt tgtaaactat gcatccatta
     3001 cctccctact ttataatcat agcatctcca aaactgaaga atctatatct ttcttttact
     3061 gcctcattat atgcatttaa aacattatcc tttccacaaa tagcacttac taacattatt
     3121 agtgtagact ctggtaagtg aaaattagta ataagtttat ctaaaacttt aaatttataa
     3181 cctggatata taaatatttc tgtccatcca ctagtttctt tcatctttcc atactcatta
     3241 caagctgatt ctatagttct acaactggta gtaccaacac atattacatt ttttccattt
     3301 tcctttgctc tatttatttt gtcagctgct tcttggctaa ccatataata ttctgaatgc
     3361 atcttgtgat ttaaaacatc ctctactttt actggtctaa aagttcctaa gccaacatgt
     3421 agagttacaa aggctatatc tacacctttt tcttttattt tatttaacaa ttcttctgta
     3481 aaatgtaatc ctgctgtagg tgctgctgct gaaccattat gctttgaata aacagtctgg
     3541 tatctttctt tttcatctag tctagcagtg atatatggag gtagtggcat atttccaagt
     3601 tcatccaata tttcttcaaa aattccatca taatggaatt ttataattct tgaaccttca
     3661 tctgataaat ctacaacttc tccaattagc tttccatttc caaaagaaaa tttagtccct
     3721 acttttgctc tttttcctgg ttttactaaa gcctgccaag tatcttcctc tgttcttttt
     3781 aataataaaa actctatttt acctccagtt tcaagcttct ctcctattaa tctagctggt
     3841 ataactctag tattatttaa tactaagcaa tctcctggat ttaaatattc tattatatct
     3901 ctaaatactt tatgttcaat attacctgtc tctttatcta ataccatcaa tcttgagcta
     3961 gctctatctt ctataggaac ttgagctata agttcctgtg gtaagtcaaa tttaaaatca
     4021 cttgttttca atgttaaact tcctctctta aaatttctcc agctacaata atatcaaaaa
     4081 aacaacttat tttccacaat attaccataa tctatcaaga aattgctatt tttaacagac
     4141 accatatcca caatatatag atatttttcc acgatataca tgtcttatcc acaatatata
     4201 gatacaatac acacaatttt tatcgtgttt atccacagta tctacagata taattaccat
     4261 tttgtggata tgtttgaatt tttagccatt ttagaaatat tttaatttaa tgactctttt
     4321 attttataaa tactcgtata atctattttt atttttcatt aggatatgga atttttaagt
     4381 gttcatatgc taatggcata gctactcttc ctctaggacc tctatttata aatcctaatt
     4441 gaagcaaata tggctcatat acatcttcta ttgtatttct atcttctccg atagaagctg
     4501 ccaatgtatc taaacccact ggtcctcctc taaatttttc tattatagtc ataagaagct
     4561 tctcatctac aaaatcgaga cccaaactat cgactcctaa aagttccaga gcatcttttg
     4621 ccaccttatc agttattttt ccatcagctc tgacttgtgc aaaatctcta actcttttta
     4681 gtaatctatt tgctatcctt ggagtccctc ttgaacgttt tgcaagttct aatgctccat
     4741 tagactgtat ctctgcatct aagatgcttg aagacctaag tactattttt gaaagttcat
     4801 caactgtata atagtccaat ttacaaataa caccaaatct atctctaagt ggatttgtaa
     4861 gcataccagc tcttgtagta gctccaataa gtgtaaattt aggaaggtca agtcttatgc
     4921 ttctagcaga tggccctttc cctattatta tatctaggca aaaatcctcc attgctggat
     4981 ataaaacttc ttcaacactt ctattgattc tatgaatctc atctataaat aaaacatcat
     5041 tttcatttaa attcgtaagt atggctgcca aatctccagc tctttctatt gcaggtcctg
     5101 acgtaattct taaatttacc cccatctcat ttgctattat acttgctagt gtagtttttc
     5161 caagacctgg tggtccatat aaaagcacat ggtctaactg ctcatttcta gatttagctg
     5221 cttctataaa tatactaagt tgttctttgg atttttcttg acccaaataa tcttctaaag
     5281 tttttggtct taaactattt tctatatcta tatcttccat cttcattgta gaagttatta
     5341 tcctattttc atcttcaaaa ccttgcattt atacccctcc tctcatactt ttgaaaatct
     5401 actttatata ttttcaaatt tacataaact ccttaaatat tgttttaaaa tccaaatatt
     5461 ataaaatcca aatactattt caaggattta ctcattatat atgtaagtgc tttctttata
     5521 atagcttctg tattcatacc atctttttta catttttgaa ctgcctcttt tgattccgac
     5581 aaagtatagc ctaaagctac aagtgcatct acagactcat cttgtgatat caaggtaggt
     5641 ttttgtgata ataatgttgg ttcaaactct atgttatttt tgtctacctt atccttaagc
     5701 tctaagacga ttctttctgc tgtcttttta ccaacaccag gagctttgga taattttcca
     5761 atgtcttcac ttaaaatata tgctcctaac tgtgctggtg atgcaaatga tagtattgaa
     5821 agcgccactt taggtcctat tttagataca gatgttagta attcaaacat cttaagttct
     5881 tccctactta caaaaccaca aagactcata tcatcttctc ttacaattaa tttagtatat
     5941 atttttgtgg cttctcccac ttgtaaattc attattgtat tggatgatac atttatttta
     6001 tatcctatcc cattattttc aacaactata ctgtctatat aaatttcttc tactgtacct
     6061 tttatataac tatacatact taccacctat attctttaga gttttttcaa gtttgtttga
     6121 atgagcatgg caaatagcca cagctaaagc atctgcaaca tcatctggtt ttggaacttt
     6181 ttttagactt aaaaatgaag ttaccatctg ttgaacttgt gccttatctg ctctcccata
     6241 tccaactaca ccttgtttta cttgaagtgg agtgtattca tatacaggct tcccattatg
     6301 agcacatgca agcatagtaa cacctctagc ttgtgcaact gttatagctg tttttacatt
     6361 cttgttaaag aataattctt ccattccaac ttcatctata ttgtaattct ttactattgt
     6421 atcaattcct ttatacacaa gttccaatct tctcgatata ttcatatggg caggtgttgt
     6481 aactgctcca taatcgatta ctttaaactt gctatttttg tattcaatta taccatatcc
     6541 aactatggct atacctgggt caatccccaa tattatcatt ttttaaaatt ccccctaaaa
     6601 ataaaagaaa taccaaaatg tatttctcat aaaaacttat gttctaaatc attatatcat
     6661 aaataatatt aacaaatcat aaaatgctct atgtaaaata taaaatcaaa taaaaaagtt
     6721 atatcaaagc aagattttac ctcaatataa cttttatatt tattacaaat aaattattgt
     6781 atttttatag gaattaaatg tgactacaat tttaatacta atctatttaa tactaatcta
     6841 tttaatatta aattatttat tattaattta cttagtattt aacttactta gtatttaact
     6901 taaaataata ttaatttact attaacttgc aaagtcttct aatcttgaat aggcctcttc
     6961 ttcagtatcc ataactacac cttttttgat ttcatcttga actgttttag gaagtgaatc
     7021 tactcttatt tttgtttttt ctactaatgt tttgcttcca ctatcatcat atttatatag
     7081 tgctatattt ccattatcat tttcaatagt atatttacct tttatagtaa catccattgc
     7141 ttgtacatct ttattatcac tatatttaga ttctaccaat atgatttcat ttttatccat
     7201 actctttatt tttttagttg gatattcttt tttaaaatat gatactattt ctgacttcgt
     7261 cttatctaat aatgactctg gaactattcc tgccattgta agtgttctct tatagttagt
     7321 ttcattttcg ccttcacttt gaagccaaac ttcacaattt ttatttaatc caaaagcttc
     7381 ttttgacttt tcattcatat tcgcactagt acttacaact tcaccagttt gaactccatt
     7441 gtgttttaat cttgtcccta tataaactcc gctacttaat acaaatactc caaccagtat
     7501 taaggcagat attttccaag gaaataattc ttttttcttc ttttcaaaca ttgattacgc
     7561 ctcctttttc tattattttc tccaagctgt aagtttttat acaatttagg taaattttag
     7621 aagaaaatat atttatattt caaattactc taagaataga tattttctaa aaggtagttt
     7681 tctcttaatg gttatattct tgtaattata tggacataat ttataataaa taatcgttta
     7741 ggaggtaata actttggaaa gaataaactg taatgtaatt aactgttcac ataacaacag
     7801 ccatatatgt tatgcagata aaatttttat aaatggtgaa aaagctcaca atgacagaca
     7861 tacttgttgt agttcatttt tagatgaatc tatttatagt aatttatcta gcaacgttaa
     7921 taatagaaat tgtgaggaat tagtttgtac tgtaaaaaat tgtaaataca acaaagatgg
     7981 tagcttatgt acacttgata atatattggt tgctcctgaa actgatagag taaaccttta
     8041 tattgaaaca tgttgctcta gtttcgaatg taaatagtca tagtataaat ataagggtgt
     8101 caaatgtaat taaactaaca tagtgtagag tatctacaac tattagtttg attatacttg
     8161 acacccttat ttattttaaa taaaatttca ttattataac catgtttata atgttctaac
     8221 tgtaattaaa tttgataaat ttctataaat tggtataatc aatatgtaaa ttgaatttag
     8281 taaattaatc ataaaaataa aatatcgagt atatagtgtt atactaacta gaaatgaggt
     8341 gcttatgagt acattttata ttcaatttct ttattttgta atttatagct ttataggatg
     8401 gtgttgtgaa actacatatt gttcagtttt acaaaaacaa tttgtaaata gaggtttttt
     8461 aaatggtcca ttttgcccta tatatggatt tggagcatta gcaatagttg ctaccttgac
     8521 tccttttgta cacactattc ctttgttgtt tttgtttagt ataattataa ctagcataat
     8581 ggagtattgt acgagcttta ttttagaaaa aatatttaat atgacttggt gggattattc
     8641 taaacataaa tttaatattc acggaagagt ttgtctggaa aactcactta tgtttggtgt
     8701 attatcattg attgtaatgc ttatagtaca tcctattgtg gttgacttta ttaactctat
     8761 ctctaaaaat gtgctattta tatttgctat atctatagaa atttattttg ttttagattt
     8821 agttatcaca gtccatacaa ttttacaatt aaatggtaag ttaaaacaaa tcaatcttat
     8881 aattaaggaa ttaaaagata agaaagaata ctacaaactt attacacaag aaactataga
     8941 aaacaaactt gaatctttag ttgaaaatag actggatact ttggaagata ggtttgaaat
     9001 acttaaagaa aaagaaaatt atcgctatgc taaaaatcgt attgatgagc taaaaaataa
     9061 acttaatact ttgctgtcta accacaaatt acttcataga cgtataatca aagcattccc
     9121 caacataagc tctaataagc atacagatat tttaaataaa atcaaagaaa atattaaatc
     9181 aattaaaaaa gataaataat aaaataaaca cataagttaa ataataaaaa tactaataca
     9241 taagattaag gtttagaata ttttttgtaa aatactctaa accctaatta atatactatt
     9301 atttttaatc ttaatttata attttagttt attttaattt taactcagta aatttaagcg
     9361 tttaaacttc aatacactaa tatactaccc catcaatatc tcaactgatt ctctaagtgt
     9421 gctaatctct cccacatttc cagggaaaat tatatatggc atattaggaa atttgctttc
     9481 tttgcctgtc atccatactg gtataccagg ttttatctgt cccattactg tagctttttt
     9541 tactcttagt gctttagtac caacatcact agaagtaatt ccacccttag ctataataaa
     9601 atttggtctt acattaagca taccaattat actggtaact gcatctgata tttctacaga
     9661 aatcataagc tgtttatctt tatcattcgt atccaagtca aaacgctctc gtctagtata
     9721 aacagcaaca gttttacccc tgcttatttt ttcctctaca acttttataa ctctttttac
     9781 ctcatttttt agaccatttt cttgaagaac taaatgttga ttaaattcta taaattctat
     9841 cggatattta caatttttaa gttcttctaa ctgttgggta gtcttattca catgtgaacc
     9901 aacaagtata attcctccat tggtattacc ttttgatact aattcatcct tagtaagtag
     9961 ttccttgtca ctcactccgc caagtatttt tgttatggca gcagcgcttc taaacataaa
    10021 ttccttacca ctatttactg ctcgtataaa cgcaattgta aataccttta catctacata
    10081 atcggttgca tttacaataa ttttattgaa tccttcagct tttttaagtt tttctgttat
    10141 agcttcaata tttagctctc taagttcttt tgacgaaata tatatcatat cactagattt
    10201 gtactttcca tttgtctttt cttcacacca ctcacctata tcagatgatt tatatccaaa
    10261 agatttatct tttgcaaatt cagtcattcc tgctggtaca agtatttctc cttctttaac
    10321 ataatgtaca ttatctatag taaacctacc accttcttta aaaaatggca ttattatttc
    10381 accctgaaat aacttctccg ataatctctc tacttcgctt tttaataaat tagtttcaag
    10441 aggataatgt cctctcatag tagaatcact acgacttatg attataaaat ccttattaac
    10501 tttctttgat acatccacaa tattctttga aatttcttta tgtgcttttg tagtttgagc
    10561 aacagtaaat ccccttgaat tggtaagtat aaaaaacata ctatttttct cattaaaacc
    10621 ttgttccata ctatccttat cccaatcagt ataaacagaa atatcatgaa ctgtttgtac
    10681 acctgttggg tcatcatcta atacaacaat ttttctatct aaatgcttaa gctcatctgc
    10741 aagaagttta tttaatctgt cctcatctac ttgtttaaaa gtattaagta tatctatgct
    10801 aattggtttt acttctctca tttaaaacgc ccctttatgt aatttgtata gtctaaatgt
    10861 aatttatatg atataaacaa tagtttgtta tttatagacc tggaagtggt ataattccat
    10921 ttataacact aagcaataaa accattataa atgctacaac tccagcaatg gttcctccaa
    10981 ttgtccaaga gtttaaagta tctttaactg ataatccact aaatcttgaa agtacccaga
    11041 atccagaatc atttggaagg gagaaaccaa ctcctccagc acatatagca agccctacta
    11101 aaactggtga cattccagtt gccaaaattg taggtcctaa aattgatgat gttgttacta
    11161 aggctacagt agcagaacct tgagaaattc taagcaaggc acttaataag aatccaagca
    11221 ctacaactga tatactaaga ccagacatag tatcaactaa gaagttacct ataccacttg
    11281 catttataac actgccaaac gcaccacctg accctgttat caataatatt agccctgctg
    11341 aatcagcagc ttcaataact acatctccca taggtttatt tatgtatttc tttaaaaatc
    11401 ctattgccac caatattcca agaagtattg ctatgttttt atctcctata aatgcaagta
    11461 ctccactcat tgtagaacct ttttctaaga acatactaaa tacatttgat ataagtataa
    11521 gaagtattgg gaacaataaa gaaaacatag aaattccaaa acctggtact ttagaagaat
    11581 catcatcttc aatttttatg ctatcttttc cctgctctaa atttttttct ctatcattct
    11641 catcaatagc ataagctgga tatttttcaa aacgttttcc ataaatccat ccacctataa
    11701 gagctgctgg aaaagcaaca attatagaat aaagtataaa actacctaca tttgcattta
    11761 catttgctgc aactgctaaa ggtcctggtg taggtattac aagtgcatga cctactatag
    11821 tcccaactcc taaggcacat acaaaaacca ttagagattt cttagttact ctcgaaacat
    11881 acttaattat aggcatcatt ataacaaaag ctgcatccat aaaaactgga attgaaatta
    11941 aaaaacctgc tgtagttact gctaaagcac tgtttttttc tccagtctta gctaacaacc
    12001 cctttgcaat agattctgtt gctcttgatt ctgaaagaat atttccaaat attattccaa
    12061 gtccaattac aatacctata ctacctaatg tatttccaaa tccagtcgat attgtggttg
    12121 atatttcatt tataggcatt ctaaccataa agcctgtaat tatagatgtt atcagtaatg
    12181 ccaaaaacgg atttacttta aatttaataa tcgatacaag taaaattcct atagatataa
    12241 caaatgctat caaaagtcca tatcctgtaa tcataatctt taccccttta tcattctact
    12301 ttattattta tcaattttat ttttctttta ctatgataac ttaacctcgc tgtatttata
    12361 taatctaatc ctaataatat gtatttaatt atattgatat atttaaatat taattattgt
    12421 gtttttttat ttcaacacct gccaactttt caaaatactg tgctattcca ctatggtcat
    12481 cttccaaatg tccatgtact tttaatgtct gcataatttc taataattga cttgtcatag
    12541 gtagtggtac atctaaattg tgtgctgttt gcattacatt cttaatatct ttcaaattaa
    12601 tagatatttt cccacctggt ttaaaatcac gattcataat cattggtatc ttcgcatcca
    12661 atattgtact accagctagg cctcctctta ttgccttata tactttttct gggtctgcac
    12721 ctgcttttgc tgcaagtaca aatgcctcag aaactgctgc tatcgtcaaa ttaacaatta
    12781 cttggtttgt aagttttgta acacttccac taccattatc tccaattaaa atagatgagc
    12841 ttcccattat attaaaaaat ggttctactt tcttataaat ttcttcctta ccaccagcca
    12901 taaaagcaag tgtaccatca atcgcttttg gctcccctcc acttactggt gagtctataa
    12961 attctaagcc catatctttt aatttatttg cacacaatat ggattctgta ggagtaactg
    13021 aactcatatc gactactata cttccttctt ttaaagtttt tgccaatcca tccatcccaa
    13081 acaatatatc ctgcacaata gttccatttg gaaggattgt aaatacaata tcacattctt
    13141 gacccatttc taaaacactt gcgtattttg ctccacatga tataagttca tccacagcag
    13201 atttattaat gtcatacacc aaaagattac atccatcttt taataaattt tttgccattg
    13261 gctttcccat tattcctaat ccaataaatc ccaatttcat tttataaccc tccatatctt
    13321 cacatttttg aaaacgtttt ttatgtttta gagtatacta cataccagta taccggtcaa
    13381 tataaaatat aaaagtattt attttaacaa taaatagtaa tttttaaagt catttattat
    13441 atttagtcat cctatataag ttttaataca tactattgtt ttcaaatata atatactact
    13501 tttacagttt tgtattttaa ctaatttcaa ttagttttaa ttaatctgcc tttaatgtta
    13561 atcatgcttc acacttcaat aattttttat attttttaat cttatctgca atttaaattg
    13621 caaaagattg accttatttt cactatgttg tatgcttaaa attatataag tttttgtatt
    13681 tttatattgt ttaggggggt attattaagt atggctacat ttaaaagatt acaagaacaa
    13741 gcatatgact acttaaaaga attgatacta tctggagaaa tgatagaaaa tgaaatatat
    13801 tctgaaacaa aacttgctag tgaaataggt atctctagaa cacctataag agatgctctt
    13861 caaagactta gtcaagatgg atttatagat ataatcccaa gcaaaggatt tagaatccat
    13921 caaattacag caaatgaaat tgtagagata ttccaaatac gctctgcaat agaaggattt
    13981 tgtacttttt taattacaag tcaatacaaa gaagctagag cagtagaaac tataagtaaa
    14041 ttaaaacatc ttttagataa acaaaaagat attttattag gtgataaaaa tttaaactca
    14101 tttgcagaat acgatacatt atttcacaca actatagttt catatgctca aaatactgaa
    14161 tttgacaaaa tgtttaacaa ttatatgtac agaataaaaa aattagctct cgattcattg
    14221 tcacatgaag gaaggcttga aacaacatta aaagagcaca cagatatttt taataatatt
    14281 gcaaatggat gtattgaaga tatttacaga actactctca ttcatatgga aactcctaaa
    14341 tacattaatt tagaagattt ttgtaacaat aagttatttt agcattatac tttcatttat
    14401 taaaaatata tctatctcaa attaatttcc taatttataa aaaatattct ataattatat
    14461 tgtaactaat acttattcta aggagtgatt tttttgaacg aagatgttgt aactaatgaa
    14521 actattgaca aagattatgc tattgaagaa gttcgtatgg catgcaaaca ttttggtgat
    14581 ttatattttt atttttccaa agtgttattt gaagagtttg gtgaagataa aactagcgaa
    14641 atattaagaa aagttttatt tgaaaggtct gaagaaagag ctattgctat gagagagaga
    14701 gctcatgaaa atggagatga attaattgca gacaatatta ttagcacaac tgatgttcca
    14761 tttctaggat gggttcctga atttgaagag ttacattgcc cctatggagc ttcctggctt
    14821 ttaagatttg aggaaaatcc ttggtttaaa aaattcgctt ctctttactg tgatgttaca
    14881 gatactactg tagctgaagt atttacagga gatacgtccc ataaaataac aaaaaacatt
    14941 ctatggggag ataaaagttg tgaaagaata tatttccatg atgacaaggt atctgaaggt
    15001 aaatatactt atggaaataa aaactgtaaa taaataattt aaattttcta tattcatata
    15061 ctatattttg tactttttca tataaatatt ataactagtc ttttatttta cttttgttta
    15121 acctaaagat aatttacttt taatctatat gaattatcat ataaataatc agaaagttat
    15181 ctttataaat tatagtttta gatttataat ttataaaata tttatgttct ttgtaaaatt
    15241 ttttagaagc ttctaatgtt atgctataaa actatcttga gaagggctga ttgaatgttt
    15301 aataaaatat atgaaaagaa agaatttata gtttttcaag ttaaaaaagg ttatgtagtt
    15361 tataatacta gaaagagttt tgaagaaggt catacacatt taaagcattt tgaagcagca
    15421 aagacagcta ttgatttagc aataaataaa aagattccta gaagcaaaga tggatattat
    15481 ctgactagtt taattcgcat aagtgatgat ggttattaca ttgataagtt aagcgaattg
    15541 ttatatgtta gagaacaaaa aggtaaaaaa gaaaagtact gtaattctgg ttatcagatg
    15601 taacaagact agttgtgaat tataagtacc aatcataagt acctttggtc ttaaaatcaa
    15661 agagtatcag taaatctata aaaaaacaac ttactgatac tctttttaat tatatgatta
    15721 atatttgaat ttgatacaaa attttataca aaagtttatg ttatctattc tgattctttg
    15781 tctgcaaatt ggctattata aagcttctca taaaatccac ccttttccat taattcatca
    15841 tgattcccct gctctataat acttccatta ttcataacta aaatcaagtc agcattgcga
    15901 attgtagaaa gtctatgtgc tataacaaaa ctagttctac cattcataag atttctcatt
    15961 gctttttgaa gcataagttc cagcctagta tcaacagaac tcgtagcttc atcaagtatc
    16021 aatattgaag ggtctgatat aaaagctcta gctatggtta aaagctgttt ttcccctaga
    16081 gatatattag atgcttcttc atttaaaaac atattgtatc catctggaag agtggttata
    16141 aagtgatgaa catttgctac ttttgctgct tgtataattt cttctttagt agctccaaat
    16201 ctaccatatt ctatattttc aaaaatggtt ccattaaata accatgtatc ttgaagcacc
    16261 ataccaaaca ttgaacgcaa atctttacgt ttcatatctc ttatatctac tccatcaatt
    16321 ttaatagcac ctttctttac atcataaaac ctcatcaata agtttattaa tgttgtttta
    16381 cctgctccag taggtccaac aatagctacc atctgaccac tttttacttc tgcatttaaa
    16441 tcttctatta aagttgaatt ttctccatat ccaaaattaa catgctcaaa tgtaacattt
    16501 ccttttacat tttctatctt aactggattt tcaacatcag gtatttcctc ttcttcgtct
    16561 aaaatttcaa atacacgttc tatagctgca aaagaagatt gtattactgt agataactga
    16621 gtcatttgtg aaagtggctg atttatctgc catatataac gcacaaatgc ttgtaaattt
    16681 cctactgtta ttcctccact tactgcatat atagaaccga caactgccac agctgcaatt
    16741 ccaaagtaag ttactaatga aaccatagga ttcatcatac ttgatataaa ttgtgcctta
    16801 aatccatttt cacaaagttc ttgatttact tttttaaatt cttttataga atcttcttct
    16861 ttaccatata gttttatttc attaaatcca gtatacattt cttgtacttt cccatttaat
    16921 tttccaagag cattttgttg caatgaaaat ctactttgag atttttttac tacaaattta
    16981 gatacaaaat aactaattgg aattataaaa agagctataa gagccatata tatattaatt
    17041 gtaaacatca ttgaaagtgc caaaattatg actaagattg agtttacaat ttgcataaaa
    17101 ctctgctgca atgcatttga tattgtatct acatcatttg atatacgact taatacatct
    17161 ccaaaactat tactatcaaa ataactaata ggcaatcttc ttattttatt ttcgacttca
    17221 tttcttaaat ctctcattgt gttttgaata gcattggtaa gtagaaaact tgaagtatac
    17281 gtaaaaattg cattggcaaa atatatacat gctaaaatca ccaatatttt tgttacataa
    17341 tcaaaattta cggaagcccc tggtactcct tttgcgatac taattacatc tttagtcaat
    17401 tgtgttatta tcaatccttc agttttaggt gcaagtgcat tcatagtagc tgctgaaatt
    17461 atacatataa tagctataat aaaggaaaat ttatatttct ttacaaatgg aagaagtcta
    17521 ccaattgttt tcttaaaacc tttagacttt ttcattattc taattcctcc ttttttaact
    17581 gagatgttgc aatttcatag tatatttcac agttttttaa taattcttta tgagttccca
    17641 ttcctacaac ttgtccttca ttgagtacta taatcttatc agcatcaata atagaactaa
    17701 ttctctgggc aacaatcaat acaattgcat cttttgtttc cttcttcaat ttagcacgaa
    17761 gtattgcatc agttttaaag tcaagtgcag aaaaactatc atcaaaaatg tatatttcag
    17821 gtcttcttat aatagccctt gcaatagaaa gtctttgttt ttgacctcct gatacatttg
    17881 ctccaccttc actaatcaat tcatcaaact ttctaggttt ttttgatata aactcatatg
    17941 cttgtgcaac ttttgcggaa tattctaatt ctgactctcc tgccttatgt ttcccaaatc
    18001 taatattatt tgctatgcta ccagtaaaaa gcagtgattt ttgaggtatg aatcctatct
    18061 tttttcttaa agcttttaaa tcatattctc ttatatctac tccatttatc ttaatactac
    18121 cttctgttac atcataaaat ctaggtatca agttaattag agtacttttc ccactaccag
    18181 tacttcctat aaaagcaact gtttcacctg tttttgcagt aaatgaaata tccttaagta
    18241 cagaagcttc tccatctgga taggtaaaag ttacattatc aaattctatg atacccttat
    18301 tttcagtatc cttaattcca ttttcaggat tttttatcaa tggttcttca tttaaaagtt
    18361 cttttattct atttgcagat acctctgctc ttggatacat aataaatacc attgaaaaaa
    18421 gcataattga aaacatggca tggaaaagat attccaaaaa tgcaaccaac tgtccaactt
    18481 gaagctctcc aatatttatc ttctcacttg aaatccaaaa tacagccaaa acagctatat
    18541 ttaatagtaa aaagaatgct ggttgagtaa ttgacataag cttaaatagt ttcttagaaa
    18601 cattagcata atatgtattt gttttttcaa atcgctctgt ttcataatca tcatttccaa
    18661 aagctctaat tactcttatt ccagttaaat tttctctcga tataaggttt aatttatcta
    18721 gtcctttttg ttgttttgaa gatattgcct gagatacttt tgctattatt gctactccaa
    18781 taattataaa aggtactgat atagccaata ctatagaaag ttccacactt gttctaattg
    18841 tcattattat acttataatg aacataactg gagttaataa ggcagtcctt agtagtatat
    18901 taacaaattg ttgaatttga aatgcatcat tcgttgttct tgtaatcatt gaagaaattc
    18961 caaatttatt gtattcagta tgtgaaaatt cttgtgattt ctcaaatatg tcatttctta
    19021 tatctctggt tacacttgtt gataccttag cagaacaata acccaacaat atactaccta
    19081 caacccctat aatagaaata ataacaatca ctattcccat ttgttttatg taagctatat
    19141 tctgatttgc aataccattg tcaattattt tagctattat agttggaatc cccaactcga
    19201 ctaatgcaaa actaaatata gccagtatgt ttaatactat taataactta taattcttta
    19261 aataacttaa aattaatttc atatttttct cctttcttaa tttttgagat agtgtaatca
    19321 tgtaataata ctaaatttat aaatgcttaa ttttataaaa aaaggagaaa agagttaacg
    19381 cactctcttc tccagcatat tctttttaat acaccctccg acaagtcgct tatatgttaa
    19441 catatttgac aagtaatgtc aattcatata gccaaaagtc ttttaaatgc ttcatttcat
    19501 aacattgtaa caaattggca tgtaatttat ttttgttttt tactgtattc caattttaca
    19561 agttctccaa tattactagc tttcttaaaa ttcaaattag tcttatctat accagactta
    19621 aatagtgaaa tccctctccc caatattatt ggcaatatat atatataata atcatcaatt
    19681 aagttatctt tcataaataa ttttactata tcgcctccac caactatcca aatatctttt
    19741 ttatccttct cttttccaat ttgctcaatt aattttttag gttctaaatt aatttatagt
    19801 gtgattattc tatgtaaaca tcactttttt actcattcaa taatacccta ttaataaatc
    19861 ctcaaactaa tttaaataaa ctttgcataa aattaaaaat atattatata aataatttaa
    19921 aaccacacta acttattgaa aattctaatt attacaaata aatattgctc tctgttaacg
    19981 ctagttctat aatataatta taagctatta atattataaa gacgatatta ctaatataat
    20041 aattataata cttatagtta atttactttt gttgaaaggt ggtaaataac atgaaaattt
    20101 cagaagtcgc tactccaaat tttttgctag atttagacca gttagaaaag aatatagata
    20161 aaattcaaca aatttgtaca aataataata aacaactttg gcctatgctt aagacacata
    20221 aaagtactta catagcgaga cttcaaaaaa atgcaggtgc tagtggtttt ctagttggaa
    20281 cacttgatga aggtgaagct ctgataaaag caggttttga agatattatg ttggcttatc
    20341 cttacatggg agaagtaaat ataaatagaa ttgtgactat ggcatcaaaa ataaatttaa
    20401 tctgtgctac agataatata gaatgtgcta aggcttatag tgaagctttt tctaaatcaa
    20461 aaatagagtg caagctactt attattgtag attgtggctt acaccgtttt ggggttgaac
    20521 cagagaaagt ggttgaactt gctactgaaa tttctaagct agaaaacata aagattgttg
    20581 gtataagttc gcatccaggt caagtgtatg gttgtagttc tccagaaggt gttccagatg
    20641 tttgtgttca agaagattct tctatggaga aagcatacaa agctctaact gataatggat
    20701 ttaatataga aattgtcgct tctggtagta ctccaacagt tgaatctgaa gctgcttcta
    20761 aaactataac tgctgtaaga cctggaaact atgtatacta tgatgctata cagataggtt
    20821 taggttgtgc tactgaagat atgtgtgctc ttactgttgt gtgcactgta atctcaaaaa
    20881 atagcagtgg atactattta attgattgtg gaagtaagtg ccttggactt gataaaggtg
    20941 cacatggaaa tagtagtatt gttggttatg gtagggtatg tggacatcca gaacttacta
    21001 ttgattcact ttcagaagaa gttggaaaaa ttaaagtaaa tggtgaaact gatataaaaa
    21061 ttggtgatag aataagaatt atacctaatc attcttgttc cactgcaaac ttgacaagta
    21121 atttattagg ttttagagga gatacaatag aaaaagtcat agaagtagat attcgtgaaa
    21181 attcaaaaca aatcatcctt taaaaacaga tttattctga tattaacatg aaagtatatt
    21241 tttcttaaaa ttatttctaa taactaatgt taatgttgga taaataaaaa acataaattc
    21301 tagaaatata aatttaaaag aagaggctgt ctcaaaatag agataagcct ctctttcatt
    21361 gttgaaatta tgaaaatata cttgatttca tacaaaaaga gtatctcatg aactaaaaag
    21421 ttcactttga gatactctct ttttttaatt aatatacaca ttaattacta tcttagttta
    21481 ttactatata tcaattacta ctcattaatc cttgtatata ttaattctta tatatcattt
    21541 atagttaggt tataatttta tgttatttac attaatccca taacagtgag taaaactctt
    21601 actattaaca tccccactat tgcatcaaat ataggaactg ccaacagtaa cattctatac
    21661 ttagtactgg catttgctac aagcactatt ctggcataat gaccaactag agtacccatt
    21721 aacatacatg gcataattaa tatggttgct tgtggtgcag tgattagacc ttgtgcaaat
    21781 aagtttgcag ctgtagctgc tcctgctgcc ttagcaaaga atgctgctat caaaactaaa
    21841 acagattccc caggtagtcc aaaaataccc attactggtc caaaaatatt tcctaaaata
    21901 tcaactaaac cagttaattg taaaaactgc actattacat aacccattat catggctggt
    21961 aatatctgtt caacacctat gtaaaaacct ttttttgctc cccccataaa tatctcaaca
    22021 gcagttttct taggtttact atttacaatt gcatttccag tattttgttg actcatacta
    22081 cacagcctcc ttagccttat tctttttatt tcttacacct atgattaatc taactagatt
    22141 agcaccaagt attttacaga atatctctat aaggattatt ggacctaatg ccaataatgc
    22201 tattggtaaa agtggagctt gagtatttat tgtgttcagt ataacagctg aaccagcata
    22261 ttgatatgct acaaatatag ttctttcatc atctgttatt tccccacttt caaataattc
    22321 tttcgtcatt attgaggcta catctgaact agtaaatgaa gatacaaatg ctattcctgc
    22381 tattccaggt ataccaagca gaggtctaag caatggatta aatatctttg atgccgcttt
    22441 cattcctccc atagattcaa ctacatctat aagacctaca gcaaaacaga cagttggaat
    22501 caaagtcaga gcaaataaga aaccatcttt tgctcccgtt cctcctttac ccataaagtc
    22561 tacaccatct gcaatagtac caaatgaacc tgtcagatta gcaaaatcta atgctttaag
    22621 gaagccttga ctatctttaa acactcctga aaataatact atcaatacaa ataaagataa
    22681 ataccctttc catgttacct tattatctcc agctgttaca tccatataaa atcccccttt
    22741 atacctcaaa tttatctaag tttaaaataa ctgtagaaat taatttcacc ctctcaaaca
    22801 tagaatctac tactgcatat tctcttgatg tgtggttcca ttcccctctt acaccaaatg
    22861 aacagattgc gggaacgttt gcaatagtca aataactagc atctgaacta cctccaagtc
    22921 tcttagcatt taattctcca aatccatttt cttttgacac ttcatttaca aatgtatgaa
    22981 atctcataac atcttctgta gtttcaaatg gcatcatttc acttataaaa ttaacttctg
    23041 tagaagttcc ttctatgtaa gtttctttac atatctctct aacttgttct ttaacattct
    23101 ccatttcttc tactttttca aatctaaggt ctacttttat actacaatct tctggaatag
    23161 aattttccac tcttccaccc ttaatcacac ttacacttac tgtagttcca acatctaaat
    23221 tagtcaattt ttgtatgcgt agtatcttat tagccatttc ttctatagca tttcttccac
    23281 cttcaaaatc attacctgcg tgtgtttcta caccttttac atgtatatta catcctattc
    23341 ttccttttct acctacacac aaactattat ctactaatcc agtttccata ttaaaagcac
    23401 aaagaccacc ttttgcttcc ctcaatataa catctgcacc tgttgaatcc ttatgtccta
    23461 tctcttcatc tccagaaaat actattttta ttggtctttc tttgtatcct attttattta
    23521 atgctttaat aacatataag gaaattatta taccaccctt catatctaaa acacctgggc
    23581 catatgcttt tccttctatt attttaaatg gattctctcc aaatgtacct gtttcaaaga
    23641 cagtatccat gtgacctgaa aaaattatag gctttttatt tatattagaa ccaagtgtac
    23701 ctatcaatgt actaccatta tctccaacat ctactaactc gcaatctaaa ccttcttttt
    23761 caaactcaaa ctttaatctc ttagccaatt tatttacgct ttctttacaa tgtgtatagc
    23821 tttctatatt aacaatctct ttccataatg aaactatttc ttctctattc tcatcaacaa
    23881 acttagaaat ttcatttaaa tatgtattca taatcttctc ctttattttc aaataaaatg
    23941 tatttctttc ctaaaatcta agtttatttt aggaaatctt aaaagactag tccttaatta
    24001 caaaatagca caattattct tgacagtcaa catatttttc agtctttttt atattttctg
    24061 atttttcttt aaattttcct tttgtaaaat aaattgacgc gttttatcta ctattataaa
    24121 atgaggttat aaattgattg attagttact atactctcga aattttaaag gaggttttat
    24181 gaaatataaa attggttttt tgacaacatc ttattttatg tcagataagt ttaaacaagc
    24241 tcttaaagaa ttaaatgata cttgtgaaat tacatttatt gagctcaaag gggagaatcc
    24301 ttttaataat ttacctaata tatactatga gcaagtagat aattttgatg gatttgtatg
    24361 cagtggtata gttccatata gtgaacttat aataaatata aaagacataa aagttccatt
    24421 aaatttttta aagcttgatg aaagagattt ttataaatac ttattcaaat tattaaatag
    24481 ccaaaaagat attgacttta caaaatcatt tatggatttt ttaagagagg acaacaatta
    24541 ttatgatata tattcattaa tagactctaa taaatgtcca tatacaatta aagattttaa
    24601 tatccctcaa cctgtatatg actttaaaaa attaaatgat aaattattgg aaatacattt
    24661 agacttattg cgagataata agattaacct ttcttttact agaaattaca tcattagttg
    24721 tgaacttaat aaacttggat atgattgtat ttacatggtt ccatctacag aatcaatcct
    24781 gaatacattt aaatctctgg tgaatgaaat aacattacaa aacttagaca aaaataaatc
    24841 tgcaacttgt atagttacag taaatagttc agagtacata aatgaagatt tgatatttaa
    24901 aaatgatgag atacaaaatc aaatatataa cactatatta aattctctat caagacatgg
    24961 attttatgga gttcaggtca aaaaaaatga tatgaaaata gagatacaca ctactaaaga
    25021 aatgttaaac aatatgacca ataactatac agattttttt atttcagaag atttaaaaga
    25081 aattaaatat agcttaaata taggttgggg cattggaaat acaaatattc atgcagagca
    25141 aaatgctcgt caagctaatg gtaaatctgc ttctttgaat ggaaactgta cttttatagt
    25201 aaatgatact aatgatacaa taggcccttt atattcaaat aaaaattcta ataatattga
    25261 agatagctca atagccaata aagttgcttc ttttatacct ctatctaata caaatgtatc
    25321 taaaatcatg tgtatgatta atgatagaaa ttcaaataat gtgtcagcag aaatattatc
    25381 aaactatatg aacataactc ttagaagtgc taatagaata ttgagtatct tatataaagc
    25441 aggaattgca actattgtaa atacaaaact cgataatcaa agaggaaagc ccaaaaaaat
    25501 atataaagtt gatttcttat cttttctaaa taagatgcag aaattaaatt cttaatattt
    25561 aattttttaa taagaacaat gtattttctc taattttaaa gaatcaaaat gtttaaattt
    25621 tattagaata tacaactttt tactttaaaa taccta
//
LOCUS       DACULQ010000037        25637 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.037, whole genome shotgun sequence.
ACCESSION   DACULQ010000037 DACULQ010000000
VERSION     DACULQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.037"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..2483)
                     /locus_tag="JA154_06240"
     CDS             complement(<1..2483)
                     /locus_tag="JA154_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAT7995462.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKEN"
     gene            complement(2497..3039)
                     /locus_tag="JA154_06245"
     CDS             complement(2497..3039)
                     /locus_tag="JA154_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7995463.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3283..4797
                     /locus_tag="JA154_06250"
     CDS             3283..4797
                     /locus_tag="JA154_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HAT7995464.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(4911..5540)
                     /locus_tag="JA154_06255"
     CDS             complement(4911..5540)
                     /locus_tag="JA154_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7995465.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRSVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            6128..6919
                     /locus_tag="JA154_06260"
     CDS             6128..6919
                     /locus_tag="JA154_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HAT7995466.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            6948..8168
                     /locus_tag="JA154_06265"
     CDS             6948..8168
                     /locus_tag="JA154_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995467.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            8188..8883
                     /locus_tag="JA154_06270"
     CDS             8188..8883
                     /locus_tag="JA154_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HAT7995468.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            8913..9899
                     /locus_tag="JA154_06275"
     CDS             8913..9899
                     /locus_tag="JA154_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KipI antagonist"
                     /protein_id="HAT7995469.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(10094..10660)
                     /locus_tag="JA154_06280"
     CDS             complement(10094..10660)
                     /locus_tag="JA154_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7995470.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            11012..12079
                     /locus_tag="JA154_06285"
     CDS             11012..12079
                     /locus_tag="JA154_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HAT7995471.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            12098..13258
                     /locus_tag="JA154_06290"
     CDS             12098..13258
                     /locus_tag="JA154_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-carotene 15,15'-monooxygenase"
                     /protein_id="HAT7995472.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPISMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            13271..15367
                     /locus_tag="JA154_06295"
     CDS             13271..15367
                     /locus_tag="JA154_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAT7995473.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(15526..15870)
                     /locus_tag="JA154_06300"
     CDS             complement(15526..15870)
                     /locus_tag="JA154_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAT7995474.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            16162..17052
                     /locus_tag="JA154_06305"
     CDS             16162..17052
                     /locus_tag="JA154_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAT7995475.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            17270..18556
                     /locus_tag="JA154_06310"
     CDS             17270..18556
                     /locus_tag="JA154_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAT7995476.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(18645..19088)
                     /locus_tag="JA154_06315"
     CDS             complement(18645..19088)
                     /locus_tag="JA154_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995477.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(19112..19906)
                     /locus_tag="JA154_06320"
     CDS             complement(19112..19906)
                     /locus_tag="JA154_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid amidase"
                     /protein_id="HAT7995478.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(20472..20948)
                     /locus_tag="JA154_06325"
     CDS             complement(20472..20948)
                     /locus_tag="JA154_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAT7995479.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(21049..22227)
                     /locus_tag="JA154_06330"
     CDS             complement(21049..22227)
                     /locus_tag="JA154_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7995480.1"
                     /translation="MCFMLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIK
                     YLNEIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKM
                     HACGHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLEKPNV
                     KYIFGLHVNPNINKNLIELKYNTLNASTNTLQLTVHGSKCHGAYPHQGIDAIVISAHI
                     ITALQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKR
                     IREICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTL
                     GAEDFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF
                     "
     gene            22381..23244
                     /locus_tag="JA154_06335"
     CDS             22381..23244
                     /locus_tag="JA154_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAT7995481.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWGKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            23315..23908
                     /locus_tag="JA154_06340"
     CDS             23315..23908
                     /locus_tag="JA154_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HAT7995482.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(24159..24794)
                     /locus_tag="JA154_06345"
     CDS             complement(24159..24794)
                     /locus_tag="JA154_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAT7995483.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(24874..25605)
                     /locus_tag="JA154_06350"
     CDS             complement(24874..25605)
                     /locus_tag="JA154_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAT7995484.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEGDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
BASE COUNT         9520 a         3110 c         4193 g         8814 t
ORIGIN      
        1 ttattttctt tatagaacaa taatattata aatgctagta gatttacaca gaatactgtt
       61 atatacatct gtttaaatcc tgagtttaga gtagattgta gtgtgttttc tatttttgtt
      121 ctattatttt ctatggaatt tatataatcc aattttgatt tatcaaaagc ctttggtatt
      181 tcagttttta cctcttgtgt ctttgctaaa actgcttgga gtgatttttt ttgctgttgc
      241 attgtatcaa ggcttttttc catgtctttc ttttggctta tagaattatt taacttgctt
      301 tgtagctctt tcttttgaga ttccattgat cttagttttt tacttaattc gttcttttga
      361 gttttatttt tttgtaactt tgtatttaag tcatctcttg tcttttttag ttgctgtatt
      421 tgagcattta aactttcttt ttgttcattt acatttggtt tattctcgct ggaattgtta
      481 ttcttatttt ctgtagaatc attcttagtg tcatctgatt cagaattact ggtattactc
      541 tggttttgtt cgtttgagtt attttcgtta tcagtaggag ttttttctgt agtaggtatt
      601 ttattataag cagttgtaag ttccttgatg gcctcatctt gtttttcaag accttgttcc
      661 atacctttta tacctttgtc aacaccagca ataccttttt ttacaccatc tattccttga
      721 ttaataccag ctctagcttt taccatattg tcaataccag tttgaactcc tttaatacca
      781 ctatttagtt tattttcacc atcttcaata ccgtttattc cactttgtgt tccatctatt
      841 cctttttgta catttttctg tattttagta attatagatg gaactttttc atcaaacatt
      901 gttgttgata tttcttttgt tatattggta atgttggttg tatctgctga ctgaacttta
      961 ttaagtaagg attttggaag tttgccacct gtcatgtcca tatttatatt tgaagtatca
     1021 ttcatatttg ggatagagac attttttaaa ttttttgcca tttcagggtc tgattttaag
     1081 tcatctatca tgttttcaag ttcttcaagt tgaactattt taggagttga gggctttcca
     1141 actaagtcca taatattatc ttgcacagat aatcctgcat gtgctataaa tccaatcatt
     1201 atagctggag aaattgaagt accaattgag cgtatcaagg ataatgttgc aagtgctgaa
     1261 ttagattctt ctaatcttgt atttgataac atcatatagt ttagaggagt tcccatagta
     1321 aatcccattc ctaatcccat aaatattaaa cttacacata cactaaatac agtattggtt
     1381 tttagagcaa ttaaaactag gtaaagagag cctatcatag atacagaaaa tcctagtaat
     1441 agtatttttt ttgccccaaa cttgtcaata agtcttccgc ttaaaggagc agcaatacct
     1501 gcaaataatc ccaatatagc tacaaaatat ccaccagaac cagcatttat ctttaatgca
     1561 ttttcagcat actgtggaac gaataccata cccatcattc caataccaac aattaaagat
     1621 aaaataagta ctatcaatat tctcggattt aaaaaatatt caaagcttaa aatagggtct
     1681 ttagctctat tttcaataaa tatgaaaata ggtataagta caataaatgt caataaatat
     1741 ggataaactg atatatcttg tatagaattt ttaaaattaa aaaagtctat attcataaga
     1801 ccatataata gtgatacgat tatacatatc aacatcaatg taccaagctt atctattttt
     1861 tctttagatt cagacttatt gtttttaata cagaaaactc caccaataag tatgattaaa
     1921 caaattggaa gatttacaaa aaatagccac ttccaatttt gagttccaaa tatacttaga
     1981 attgtgctac caatgcttga acctaaaata ttggcaatac catatgttgc cccaactaaa
     2041 cctaaagcca tgcctctttt gttttctggg aaggtagttc caaattctgc tgtagcaatt
     2101 ggcatgattc caccaccacc aattgcttga ataacacgac caattaataa aatatagaaa
     2161 ttggaaaata gtgatgataa tccacatatt aatgaaccac taccaaatag aaatatacta
     2221 actagataca catattttct tcctatttta tctgctaatt ttccagagat aggaattaca
     2281 gaagcgtatg ttaatgtgta tatggtaatc atccaaattc ccgttttttc atttactcca
     2341 aggctatttt gtataatcgt tcttgctggt gatactattc cagtatcaat agctcccata
     2401 aatataccta atagataaag tgtaagtact aaaaatattt ttggattact gtccttgttc
     2461 ttaatactag tattatgatt caaggtaaac accaccttaa tgtattaatt tttcaatata
     2521 gggttttaaa tcttcaaatt ctatatgaag atatgttgaa tatgaaaaaa acatttttat
     2581 tatgatattg catactaatt tgtcattatt aaaaaaactt tctttttcta aagcttcata
     2641 tactttattt gaaagctgtt catgaaagtc gtctttgtgt tttttcattt caaaaaatct
     2701 ctctgtttca aaagtgttta aaaagtcatt gtattggata ttatgaactt tgtttataaa
     2761 atttcttatt tcagtaaaaa taatgtttag tttttttatg taatcttcag tgtttttatt
     2821 ggagtattca attcgcctta tcaataaatc ccattcagat tttgtgatat attctattat
     2881 ttcttgtttt gaattaaagt aattatacac tgtacctgca cttataccac acctttgagc
     2941 tattttcctt atgctaatat tcgagtaatc ttcttctaaa atcatatccc gagacacttt
     3001 taatatatct tcttttacat tttctaaaat ctttggcata ttaccacctc ttttattaaa
     3061 attattatga acactgttta ttatactcct ttaaaattta ttaatcaata tgtaagctat
     3121 aatgtataat attattatat agatatgata gaaaataagt gtcttcttac taaatatcta
     3181 ttatattaaa tttgtactta agtatgatta tttattgaaa tatgtgatat taaatttttc
     3241 tttttaataa attaaatatt atatgataag gagaatatgg tgatggaaaa gaagtatgta
     3301 atggcattag accaaggaac aaccagttca agagctatac tatttaataa aaaaggcgaa
     3361 atagtgaaaa ttgcacaaaa agagttcaat caaatttatc caaaagctgg atgggtagag
     3421 catgacccta tggaaatatg ggggtcacaa agtggggtaa tgagagaagt tatagaaaca
     3481 gcaggtataa gaccagaaga aattgcttca atagggataa ctaatcaaag agagactacg
     3541 gttgtttgga gtaggtatac tggtaaacct atttataacg caatagtatg gcagtgtagg
     3601 agaacgtcag aaatatgtga tgaacttaaa aataaaggat tggaagaatc aataaaagaa
     3661 aaaacaggtc ttttaataga tgcatacttt tcagcaacaa aagtaaaatg gatattagat
     3721 aatgtagaag gagctaggga aaaagcagaa caaggagagt tattatttgg aactatagat
     3781 acatggttaa tatggaattt aactcgtgga aaagtacatg taacagatta ctcaaatgca
     3841 tctagaacaa tgatgtacaa tataaatgat ttagaatggg atgaggatat attacgagaa
     3901 ttggacatac caataagcat gttgccatta gtaaaacctt ctagctatgt ttatggtcat
     3961 acagatgagc gcatgttatc aggagctaaa atacctattg cgggatgtgc tggagaccaa
     4021 caagcagcat tatttggtca aaattgtgtt gaagaaggta ctgcaaaaaa tacttatgga
     4081 acgggttgtt tcttacttat gaatacaggc agtaacatag ttaaatctaa gcatgggtta
     4141 cttacaacaa ttgcttgggg agttgatgga aaagtaacat atgcattaga aggaagtata
     4201 tttattggag gagcatctgt tcaatggctt agagatgaat taaagataat tgaatctgca
     4261 aaagatagtg aaatgtatgc aaatagggta gaggatacta atggtgtata tgtagtgcca
     4321 gcatttacag gtcttggagc tccatattgg gatatgtatg ctaggggttc aattttagga
     4381 cttacaagag gagcaaaaaa agaacatata gtaagagcca cattggaatc aattgcttat
     4441 cagacaaagg atgttttaga agcaatgcaa aacgattcaa aattaaaact aaaatcatta
     4501 aaagttgatg gtggagcaag taataataat tttttaatgc aatttcaatc agatatatta
     4561 aatgtagata tagatagacc caaaatagta gaaactacag ctttaggagc tgcttattta
     4621 gcagggttgt ctgttggatt ctatattgga agaaatgaaa ttacttcaaa gtggtcagta
     4681 gaaaaagagt ttaatccaaa tatgagtgaa gagaagagat gtaaattata taaaggatgg
     4741 aaaaaagctg tatcgagagc tttaagctgg gaaaaagaag atgaattgga tatttaaaaa
     4801 ttaatcaaag ttaaatttat ttgaatttaa aaagagcttt atgtaaaata tgaagctctt
     4861 tttttgttgt taattttgca tttttatata atcatactaa attatataat ttatttttcg
     4921 ttttcatata aaaacattgg tatagaattg taaatgtgtt ggtcaccaac tactaagaga
     4981 atgtctcctt ctaaaaattc aatatatggt ccaggagata ttataagttc ttcacctctt
     5041 ttaattgcaa ctattgtagc acctgtattt tgccaaaatt tagtttgtgc agctgtttga
     5101 ccaatatatt tgcaattaga atttatggtg aactgaacgg gcactaaagg attgattatt
     5161 tcaagtctgt tagaatagtc taaaatttta tttatagtat ctaatatttc attttctaaa
     5221 ttagatttag tatctattaa attataaagt gttgatttat atgatgatat atttgtaata
     5281 tctctatatt tttttataaa agaagaagct ttgtctggag atagtacaat aacaccttta
     5341 ccctttatgc ttttaacaac gccaatgtcc tctaaaattt tgacagatct tcttatcgtt
     5401 tctggagata cattgtattt accagccaaa acagagcgtc caaatagtac tgaatcctct
     5461 tgtatttcac ccgtgtatat tttatttgca atatccaacg ctattttttg gtatattggc
     5521 atcgtatagt ttttatccat tgtttttccc ccaatacaaa atttatgtta aaaaaatata
     5581 taatcttaac aatactattg tataatttat aggtttatat gtctacaata tgtacaaata
     5641 ttgtgcatgt tttaaaataa tattttaaca tgcatttagt atacatgatt ttattatact
     5701 taaaactata taaagaatta attataaaaa tataaagata ttttacttac aaagaatgat
     5761 aattatatag atgaaagaaa caaatgaatg aagaaaaatg attctgtctt gcatataaag
     5821 tgattaaaaa tgaaaaatac tttaaaaata aaggttaaag cccttatttt aagtgaaaat
     5881 aattaattat taactaaaaa atggtaaaaa gattttttga aaaagaaata caaatgaatg
     5941 ttcaattaaa ataattgcaa aaatattcct taaatggtaa aattatatta attaaatgca
     6001 atagaatagt tgcaaaaata aaattgaata caagtgataa aatatttcat gcaatatata
     6061 aaataaacaa aatttaaaaa ttaaaactac ttattctatc aaaatgttag caaggagggg
     6121 tcataaattg tataaagtag atttaaatag tgatttagga gaaagttttg gaacgtataa
     6181 gattggttta gatgaagaag ttttaaaata tatttcatca gctaatatag cttgtgggtt
     6241 tcatgcaggt gaccctagtc atatggaaaa aacagtacag ctagctaaaa aaaatggagt
     6301 gaaaataggc gcacatccag gttttctaga tttaatagga tttggaagac gtgagatgaa
     6361 aataacaaag caggaagcta aagattatac aaaatatcaa ttgggtgctt taatggcatt
     6421 tgcaagttca aatggatgta atatacaaca tgtaaaacca catggagcat tatacaatat
     6481 ggcagctaaa gataaagagt tagctatggg gatttgtgaa gctatatatg aagtcgataa
     6541 agatattata ctgttggggc tatataatag tgaaatgata aattctgcta aagaaattgg
     6601 acttaggttt gcaaatgaag tatttgcaga tagagcttat gataataatg gttttttggt
     6661 tccaagaaat gtagaaggag cagttataca tgatacaaaa catgcaatag atagggttgt
     6721 taggatggta aaagaaggaa ctgtagaaac tttaacaggt gaagttatac atatcaaagc
     6781 agattctata tgtgtacatg gggacaatcc aaaagcaata gagtttgtca aagaaataag
     6841 aaagcgtttt gaattagaaa gtatagaagt ttgtccatta gaaaacatag aagtttgttc
     6901 gtcagagaac atagtataag actgataaca tgagggggag ataaaatatg agtacaaaaa
     6961 atattacaga taaaactaaa aataaaaaag atgtcggagc acttataggg gctgcattta
     7021 taatggcaac atcagcaatt ggaccagggt ttttaactca gactgcacaa tttacccaag
     7081 attttggacc gaatttttca tttgtaattt taattacaac aatccttttt ataggggctc
     7141 aagtaaatgt atggagagta attggtgtat caggacttag aggtcaggat atagcaaata
     7201 aaataatacc aggattaggt tatttagtag cttttttagt agctcttgga ggattagctt
     7261 ttaatatagg aaatgttggt ggagctgcac ttggtatgaa tgtaatgttt aatatgaata
     7321 tgacattagg aactgtatta agtggattaa tagccatatt tgtatttatg tcaaaaaatt
     7381 caaattctct agtcgataag ataactaaat ttttagcatt aggaatgata attatagttg
     7441 gatatgtagc aatatcaaat catcctcctg ttggagaggc agtatcaaga atggtaaaac
     7501 cagaaaatcc aaaaggactg atattcccaa taataactct acttggagga agtgttggag
     7561 gttatataac ttttgctgga ggacatagat taatagacgg tggaataaca ggagaagaaa
     7621 atataaaaga aataactaaa tcttctcttt tgggtatatt agttgccaca atgatgagag
     7681 ttcttttatt tttagcaata ctggcagtgg tttcaaaagg tcttcaactt gacccagaaa
     7741 atccggctgc ttcagctttt aaatttagtg caggagcaat aggatataaa ttctttggac
     7801 ttgtattatg gtctgctgca attacatcag ttataggagc tgcttataca tcagtttctt
     7861 tcttaaagac actaaatcct tttatagata aatatgaaaa atattttata atagcattta
     7921 tagcaatatc aacacttata atggcattta taggaaaacc agctacttta ttaattttag
     7981 caggagcttt aaatggattg atacttccaa tcacattagg aattatgctt atagcatcta
     8041 aaagaaaaga tattgttgga gattacaagc atcctacatg gcttttaata tttggattaa
     8101 tagtagtttt aatatctgca tatacaggaa taacttctct tagcagccta ggtgctttat
     8161 ttgcttaatt tagaagaggt gacatttatg gaaactagat acttgatatc aggggataag
     8221 gcagtggttg ctgaatttgg aaatgagata tcagaagata taaacaaaaa agtaatttca
     8281 tttatgagag caatagaaat atcgaatctt aaaggtattg taacagaaat ggtaccaaca
     8341 tataggtcac ttatgataag ttataatcca ttagaaatag attttgatag tttgattgag
     8401 aatttaaaaa aaatagaaga taatttggaa agtatagtat taccaaaacc taaaatacat
     8461 gagattccag tatgttatga tgaggttttt ggtatagata ttaaaaacgt tgcaagctac
     8521 aataatctca ctgtagatga agttataaaa atacacacaa gcagagagta cttgatatat
     8581 atgctaggtt ttactcctgg atttccatat ctaggtggta tggatgaaag aatagctaca
     8641 cctagattag aagtgccaag gactaaaatt tatggtggga gtgttggaat agcaggttcc
     8701 cagactggtg tatatcctat tgatagtcct ggtggatggc aaataattgg taggacacct
     8761 ttaaaattat atgatgaaaa tagagaagaa cagattttgc ttagagctgg agattttata
     8821 aaatttgttc caataacatt agatgaattt atagaaatcg aaaaaaataa cttaagtatt
     8881 taaatatcgt tatagtacag ggagtggtat atatgggatt caaagttgaa ttaggaggtt
     8941 tccaaacatt aatacaagac agaggtagag ttggctatgg tcaatatggt gtatctggat
     9001 gtggagcaat ggatgagtat gcacatagag taggaaatat attagtagga aattctgaag
     9061 atgaggcttc tttagaagtt ttaatgttag gtcctacaat tacctttgat gaatatacgc
     9121 aaatagcagt aactggggga gatttagggg ctaaaataaa tggaaaagaa attcaaaact
     9181 ggaggtcata tcaaataaat ccaggtgatg tattatcatt tagaggtgtg aaatcaggtg
     9241 ctagagccta tgtgtcaata gctggtggaa tagatgttcc acttgcaatg ggaagtaagt
     9301 caacttatac aagagctaaa attggtggat ttgaaggtag agcacttaaa aaaggagatt
     9361 atataaatac ttttattcag gaaaaagatt ttacaataaa taaaaaatta agttcaaaat
     9421 atattccaac ttatagttca gaaatcgtac taagaattgt aaaaggtcct caatttgatg
     9481 ctttttctaa cggtgaagta gagaaatttt tatcaaataa atacaaagtt acgaatgaaa
     9541 tagatagaat gggttgtaga ctagatggag agagtataaa gcaccttaat ggagcagata
     9601 ttatatctga tggtatttct tatggagcta tacaagtacc tggtcatgga aaaccgatta
     9661 taatgttatc agatagacag actagtggtg gctatactaa gatagggaac gttataagtg
     9721 tagatttgta taagttagca caagcaaagc caaatgatgt agtaaaattt gaacttgtag
     9781 atatatatga agcacataga ctattgagag agcaagaaga taagattcaa gatatctaca
     9841 agagtatgaa aaatataaga gttgtaaagg caaaagtatt aaatgatata gcagtttaat
     9901 tttaacaaat acaattttta ttataattta taatataact tacaaatagc tatttactat
     9961 ttaccataat taagatggtt tagattagtc aaatagctat ttttttagtt tgagtaaata
    10021 ttttaaattt aaaataaaag caatatttaa atctattata ttatctacaa aaagtaggtg
    10081 tgtcatattg agtttattta gattttattg gccaaattgg aggtgatata actccatatc
    10141 cacgagctat tgtatcttta cttggattga tgattttatg tggtacatta gcttttataa
    10201 atgttgagtc acctggattt aatctaacta ccttttcacc catacaaata tcaaccacac
    10261 cactcgttac caccataatt tcttctgatg catgatatat ataatctttg gcatcctcac
    10321 tttctggttt aagttcaaac tctacaaata aaattgaagg tgtaaactca gttgtaggca
    10381 ttggagatac tatctcgtaa aacatttcac tctttggaaa tttcatcata actctatctt
    10441 cttttctaat gactaaatca tcagtattat tgtcatccat aaacatatat aaaggagtgt
    10501 ctaaaacttc agcgattttt ctcaatgaag acaaggatgg ttcaatagca tctctttcta
    10561 tttgagagat ataacttata gataaatctg tcaatttgga aacatcactt aatgtttttt
    10621 tcttgctttt tcttatattt ctcatctttt cacctagcat aagaacctcc aaataataaa
    10681 attaatataa aatattcgct taaaacaaag taattatagc acaacataga agtagtggca
    10741 ataagaatac atagtagatt ttgacaatta aaatttaatt aaatccaaat aaaagtctat
    10801 gaaaatgaat tattgttaaa ttaatacaaa gaaaataata ataaaaaaat ttaatagaag
    10861 agtattaatt gaattaagat tatcacatta tattttggaa aagagtaaat tttcagtaaa
    10921 ttatcttgac atatatattg aagtaataat ataatttaaa gtataaaatt ttaaaaattg
    10981 aattataact tcaaaaaata aggagggtaa tatgaagata acagatatca aatttgaaaa
    11041 gcttagaatt aaattaaaaa aacctgtagt agtttcattt ggagtaattg agtatggaga
    11101 aagtataatt ttaaaaattg aaactgatga agggtattgt ggatttggcg aagcagcacc
    11161 tcttgcagca gttacaggtg aagttttgga taatgttctt tcagtattgc ttatgtttaa
    11221 aaaagagtta ataggcaaag acccactaga tattgaaacc atacatacca tcatggatgg
    11281 aattattata ggtaatactt ctgcaaaagc agctattgac atagctttat atgatattaa
    11341 aggaaaaatt atgaatgcac ctctttataa agttcttgga ggatttgata gtaaagtcca
    11401 aacggatatt actataagta tagataagcc agaaaaaatg gcagtagaag ctttagaaag
    11461 agtaaaagaa ggttttagga tattgaagct taaagcaggt ataaatcctg aagacgatat
    11521 tgaagctgta aagttaatta gagaagccgt tggagagagt ataagaatta gaattgatgc
    11581 aaatcaagga tggaacgtaa atagcagtat aaatactatc aaaaagttag aagaatttaa
    11641 tgttgatgca attgaacaag cacttccaca ttgggattta gatggtactg cttacataag
    11701 aaataaaagt aacacaaaaa ttatgataga tgaatcatta cattcaccaa tagatgcaat
    11761 taaggctata aaaaagaatg cagttgatac atttaatatt aagctgatga aatcaagtgg
    11821 aatatatcct gctataaaaa tcaacaacat agctgaggca agtggagtaa attgtatgtt
    11881 aggatgtatg ctggaaacga gaattggtat aacagcagca gcaaatttaa tagcatctaa
    11941 aaagaacata acagaagctg accttgatag ttttatgttt tgtgaagaat tagaaggtat
    12001 atctggagga tttgtaatgg atggagatat tatgaatctt gtcaataaac caggtttagg
    12061 aatagaagta aacttatgat aaatttggag gttaaatatg aatacacttt tttcaaatcc
    12121 aggtagtata cttgctgtaa tgacatcaat gattgcattg ggtttttact tacaaagata
    12181 caaagccatt aaaagcttag gaccagcact tacaataatc ataatgggaa taatactttc
    12241 aaacttaaaa gtagttccag taagtactga gttatatgga actatttcaa catatgctat
    12301 tccaatttct atgactataa tgttgatgag tgttgactta aaagaaatga caaaattatc
    12361 aagagaacct ttaattgcaa tttttgtagc tgtattaact gtaagtatca tggcattttt
    12421 atttggatta gtatttgcag aaaaaatatc agaaggttgg aaggttgctg gaatgtttgt
    12481 aggtacatat actggtggaa gtgctaatct tacagctatt ggaacaggtc ttaatgtaag
    12541 tagacaaact cttgcagcag caaatgcagc agactatgta attggagtac caacattgat
    12601 atttatgttt gctctaccag caatattaaa aaattcaaag aagtttaaaa aactttggcc
    12661 atatcatgta gaagaatcag aattagaaga ttgtcaaaat gaagaattta tggagtcaaa
    12721 agaatggagt attaaagata tagcttggat gttggctata ggttttgttg ttacagaagt
    12781 ttcaacaata cttgctggat atttcaattc aagttttagt agtgcagcaa gaatactttt
    12841 agttacaaca atttcaatta taattgctca attaaagcca gtaaagaaac taaagggaaa
    12901 tttagattta ggattatttg ttgcattatt ctttttatgt acaataggtt tttcagtaga
    12961 tataaaagag tttttaggtt caacttttac tataacactt tactgtttta gtataatttt
    13021 tgcttcattt gttttccatt taggcataac tagactttta aagataaagt atcaatatgt
    13081 aatactttct atagtaggag caattgctga tggtcctact tcagctttag tagctgcttc
    13141 tgcaaagtgg aattcgcttg taagtgtagc agtcgtaatg ggagtaattg gtggagtatt
    13201 aggaaattat gcaggtatat cagtggcata tgcaataaag atgtctctag gattataggg
    13261 gggagtttaa ttgataaaat ttaaattata catatctgga ctatacagtg gaaatgtaat
    13321 ctttgatgga gatttattaa ttgaaaagtt gaatcctttt acaaataaaa tagaaagttt
    13381 aaaaccaata agcaaagaag aaaatacata ttatttaaat cttacaaaaa tagatttaaa
    13441 gtctttattt aataattttg atgtatatac taaaagctta gtaaacacag ataaagacac
    13501 agttataaat aacttaggag aaacgcattc taaacttaat gaatatatat gggttcaaag
    13561 aaacaaaaag ttcccactag atataattat tgtagataat aaaatagtag gttttatctg
    13621 tttatctaga gaaacttgta ctatattaat tatggatgga tatgaagagt atactgtatt
    13681 aaaagaatgg gaaaaaactc ataaaaatga agagatatat tctataagat ttggtggaaa
    13741 ttatatgatt gatatgaaag atggtataaa actttctaca gatgtatatc tccctgattt
    13801 tgtagattct gccaaaaaag caccgacaat tcttatgaga actccatatg gaaaagaaaa
    13861 tgataaagaa atatattata aatatgtaca aagaggatat gctgttgtaa tacaagatgt
    13921 taggggaaga aatgaatcag aaggtaagtg ggaacctatg attcatgaga gggaagatgg
    13981 agatagtacc ataaattgga tagtatcaca ggaatggtca agtggaatag ttggaatgct
    14041 tggagcatca tatcttggtt atgtccaatg ggcagctgca tcttcaggca ataagcatct
    14101 taaagcttta gttagtatag taacatcagg aagccctttt attgatatac caagaaaagg
    14161 aggagctttt gtatctggta tgcttgcatg ggcatttatg gtatcaagaa ataaagttga
    14221 tagaagcaaa atggtaagag atgattggga tgatgtttta aacatacgac caatagaaaa
    14281 tatacctgtt gaagctcttg gatatagaat tgaatttctg gaagaatggt taaagagagt
    14341 agaaaaagat gagtattggg atttgatgga ctggcattta caaaaagata aaataaatgt
    14401 acctgcattg gttgtatctg ggtggtatga tgataatagt atgggaacta cagaggcctt
    14461 agatgttata aaggactatg aaaaaggaaa gagaaaggct attctaggtc catggatgca
    14521 taattcaaat actttaagag atataaatgg tatttcttta ggaaatagtt cattaagata
    14581 tgatttggat tataattatc tattatggtt tgataaatac ttaaaaggta tagaaaataa
    14641 catagatacg actgctccag tagagtatta ctctgtaggt tctaataagt ggaagacaga
    14701 agaaaattgg ccaataataa ataagataga taaaagtatg tatctaataa gtgatggaaa
    14761 tgctaacaca tctttaggaa atggtagatt agtatttgat aatgatttag aagaaaagta
    14821 tgatagttat atatataatc caaaagaccc atcagtacaa ctaattgata tgtcagagaa
    14881 tgaagttgga gttccaaata actacaaaga tttagaaaaa agaagtgata tgttgtgtta
    14941 tacatcagat gcttttagtg aagaatttac tgtaacaggt gatataaagt tagagttttt
    15001 tgcatcaagt tctgccaaag atactgactg ggttataaaa attatggacg tggatttaga
    15061 tggaaattca attaaattag ctgatggtat acttagtgct agatttagaa atagttttta
    15121 taagtcagag tttatggaag aaggagagat ttataagttt acgattataa cttcaaaaat
    15181 atcaaacacg tttaaagttg gtcataaaat tagactagat ataacgtcta gtgctaaaaa
    15241 ctttattttc caaaatagca acacgacaga gggatataat agtattgaat atatagaagc
    15301 aaagaatacg atttaccatg gaggtaaata tccatctaaa ttgatattgc caatagagaa
    15361 taagtaaatt tatatagatt aaatagtgag gaggtgtctc aaaatgaatt ttttagttcg
    15421 tgagacactt tcttttatac gaaatcaaat atattttcat catttcaaca atgaaaaaga
    15481 ggcttatctc tctgttttga gatagcctcc tataagctaa attgattatt ttgtagctgc
    15541 aacaacgctc atttcaacta acaaatgctc tctagcaagt ctagcttcaa cacaagctct
    15601 agcaggttca aatccttttt caacccaagc atcccaaaca gagttcatag cttcaaagtc
    15661 tttcatatct cttaaataaa tagtaacaga tagcaaatgt tgcttatctg aaccatattt
    15721 gtttaataaa tcttctattt tagctaaaac ttcagttgtt tgttcaatta ctcctccttc
    15781 agcatgagtt tgtccacata aataaactgt attgttatga actacagctc tactcattct
    15841 tccagttcct tcatatctct ttatttccat attttaaata cctccatagt taaaaaatct
    15901 attaaaaatc taaataataa attaaaaaaa gtctatacta tataaaatta tattatatat
    15961 tctgataata atccataaaa acatataact ttttagctat aattattatt gttattaatt
    16021 gaaaagattt attagttact tgacatagtt tacactttta attatagtat aaattggaat
    16081 atttagataa ttattattta atgtataagc taagttttta attaatatgg ctttatacat
    16141 ttatatagaa aagaggtacg tatgagtagc acaatcaaat caaatcgaat aaaaggtata
    16201 atgtttataa tagcatcagc gcttggcttt gcaatgatgt ctgcattcgt aaagctagct
    16261 ggagatttac caagttttca aaaagtgttc tttagaaatc tggtatcagc tataatagct
    16321 ttttggctta tcataaaaca taaaggaagt ctaactggta aaaaagaaaa tttaaaagta
    16381 cttttatata ggtcaatatt tggaactcta ggagttatat ttaactacta tgcaattgac
    16441 aagttgatgc tttcagatgc taatatgcta aataaaatga gtcccttcct tgtagtaata
    16501 ttttgtgcaa ttgctctaaa agaaaaaata aatgtaaagc agataggagc tattataatt
    16561 gcatttatag gagctttatt cataataaaa ccaacattta gtgtagaagt aattccatat
    16621 cttggaggaa ttgcaggagc tgtatttgca gcaatggcat atacttgttt aagagttctt
    16681 ggagatagag aagattacta tacaattgta tttttctttt cagttttttc attagttact
    16741 gtagggccaa ttgcttttgc agtatatgaa ccaatgaccc ttatgcaact tatatatcta
    16801 ttactaggtg gagtatttgc aagtctaggt caatttggaa taactctagc atataaatat
    16861 gcacctgcta aggaaatctc tatatttgac tatagtaata taatcttttc agcaatttta
    16921 agtatattct tattcaatgt ctatccagat atgttaagtg ttgttggata tttaatagta
    16981 ttttcggcag cattttatat gtttttatac aataagaagt tagataaatt agacaaagaa
    17041 aagaagaagt aaactagata aaagtgtttt tataatttgt atttaatatt taagtctttg
    17101 tattaaaatt aaagaaaatt taattaaaaa tgtttggtat attaacttat aacagcagtt
    17161 aaaattgaaa aattataact gctgtttctt ttattaaaaa tatccaaatg tctgtaaaaa
    17221 tagactataa tagagtagta taaataatat aaaggggata aattaaagta tggaaaaagt
    17281 gaaagttgct gtgattggag caggtaatag aggaacgtat gcatatgctc catatattta
    17341 tgaaaattct gatgtatgtg aaatagttgc tgtagcagaa cctaaaaaag gaagacgaga
    17401 actatttaca caaaaatata acctagatag taaaaatgta tttgaaactt tagaagactt
    17461 ttttaaacat gacaagatgg cagatgcagt aataatagct acaaatgatg atagacatta
    17521 tgatgtagca aaactagctc ttgaaaaagg ttatcatgta ctattagaaa agccaatgtc
    17581 taatagttta gatggtctgg tacatataga tgacttgtgt gataagtata aagataagat
    17641 ttttatgatt tgccatgtac tgagatattc tccattttat aataaattaa aagaaattgt
    17701 agaaagcaaa aaattaggag agctagttag tatacaatat aatgaaaata taggatattg
    17761 gcattttgca catagcttta ctagagggaa ttggagaaat agcaatgaaa ccagcccttt
    17821 aattttagca aagagttgtc atgacatgga tattttgtta tatttagtag gaagtagatg
    17881 caaaaaaata tctgcatttg gaagcttaaa gcatcttaat aaccaaaatg ctagtggaga
    17941 gatggcacaa aattgtcttc agtgtttagt tgaaaaaaaa tgtccatatt cagcaaagag
    18001 aatttattta gaaaaagata gaagtataaa tagagctgtt cacataaatc caactgaaga
    18061 gaatctattg aatatactta agacaagtcc atatggaaga tgtgtttaca gatgtgataa
    18121 taatgttgta gacaatatgg taaatatact acagtttgaa aatggagtga cggctacttt
    18181 taatttatgt gcatttacta aagaaaatgg aagaactata aagttaatgt ttagccatgg
    18241 tgaagttggt ggggatttaa ataaaaatga gataagaatt aaagaatttg gaaaaaacga
    18301 agaaatagtt atgaatccat caaatcaaca gaatatggta gaggaatatg atagaaatct
    18361 tatagctgaa tttattaaat tagtttcaaa taaagaacta gaaaagggta gagtagcagc
    18421 aaaagaagct atacaaagtc atgtaatggc atttgcagca gaatattcta gggtcagtga
    18481 tgaagtagtg tatatagaag aattttttga tagtgctaag caaatgacta aggagataga
    18541 agaaactata ttttaattaa taaaaattta ttaaataaaa taaagataga ttaaataata
    18601 gaaatacgaa aatatgtgtt ctattaagga atctatcttt tttattaagg ttttttctta
    18661 tccctatcaa aaaaatccat acattctgat aaaagataag aaagtaaagc gaataagatt
    18721 tctgtactta aaggaatcgt tactgaatcc ataaaggtat caattatgtt catagctaga
    18781 aaagttagta taaaatcaat tataaattcg caaagtttat atattgagta tggtaatttg
    18841 tttacatatc tgaatatatc taaaatagta gtgcatagaa aatcaattgg agtggttata
    18901 caaaaatata ttaaaaagaa aattaataca gctccaagag atttatattc aaatcctaag
    18961 aatgataaga agccaattcc tataaccgtg aaaatacaaa aagttatacc aaaaaaaaca
    19021 gcaaaaaata aaaatacaga aataaattcg cctaaaggaa tccttctttt attacttatc
    19081 ttaatcaact ccttaattta tgatatgaaa tttatataaa taataacatt cttaaaaatt
    19141 cataatagtc ctctgtttca aatagtactg tttgagaatc tatttttttc atatttggat
    19201 aaaaagaggc cttaaaagct ttataatgtt ctctaaattt tatttctatc ttaaatttat
    19261 ctggcaattt tattaagtgc ctagataaat ctccagataa agcttttttg acaccttttt
    19321 caattttttt tagtgcaagg tttggatgga ttgaaattga accatttcca attccttttg
    19381 acacaggaac agtgactata tttggattta gtttttttgc actctcacaa agcatttcat
    19441 ctccacttaa aaatgctaca ggaacattat aatatgcagc aatataagca tttatagtaa
    19501 attcagatgc tatttcatca tttattttaa aatagtcata atcaatatcc atagtatgag
    19561 aaagtggatt accattacaa gtagctccag agtgatagcc ggtgaatata catgcatcaa
    19621 agctttcatc aattcctgcc atcataacta gtgggtcttt ggtccatcct ctaagtattt
    19681 tgacgttttc aggtaaaata tttggattaa tatttctacc actgtcatgt gcatctttaa
    19741 caagtatttc atgtacacca gattgagtag ctccatcaca tgctgctgtt atctctttac
    19801 tcatttgcgt tctatagtgt tgcgaaaatt cacattcaag ttttgtttca tcccaattta
    19861 caataccaca agtaccttct atatcagcac ttaaatacaa tttcataata tataccccct
    19921 tatgtttatt attgatgata ataacagata acagaaaata cataatcaat aaagggcata
    19981 aaaatattat gtcttactat taactattat taaatctgta actgaaaaat aactaaattg
    20041 gtataaaaaa atataacact atgtgtcatt tatttatata gttttttata tttgatgttt
    20101 atataaaatt gtaacatata agtggttaca tttactagtt taacatagtt aaaatagtta
    20161 ggtattattt ttaagttaaa ttgattatta taaaatcgca gaataaaaga aaaatttaat
    20221 ttattaaaat gcatgaaatg ttttttcctc aacataatgc taaattaaac tcaaaaagaa
    20281 ataatagaaa aatcttatgt agcttagaaa gaatgtgaat gatttaaggg aaaaatagaa
    20341 atttattata taatattagg ttgtattata taatacagcc taatattata tgtatatgtg
    20401 acataaaatc ttggtaagtg taaataaaat aaatgtggaa aacaagttaa tgaaatcaag
    20461 aaaaaagtaa tttaaaattc aatttcatct tcccaattaa attctcctga tttttctaca
    20521 ctcttaggcc agtgactaca ccatttagga acggacggat tttcaaccct atcaacgctt
    20581 ggagtatatg gctttaagtc aataacagga ctattatcat tagcatcaat atatggtatt
    20641 tttattatgc cattttcatg gtcgatattg ataatttgta cagcagtcaa tgctattgga
    20701 ttgggtctta taggtgacct tgttgcaaat attcctataa cgtctggtcc acttttataa
    20761 ggttttgggg tttctaaaat acttcttagt tcaggattat caaacccatc agcccaccaa
    20821 aatacattta aatgactgaa atcgtcgagt tcatttaatg caggtacata ctttttgtct
    20881 aatttaataa agacctcttc gccttgaata catattttac ctataggatt tattaatatt
    20941 tctttcataa tattacctcc aactatttaa tttatcattt gtaattttag ggtataacat
    21001 gacatcatgt gagggtcaat ataaaataat aaataaaaat attaatagtt agaatgataa
    21061 tacatttttt acatggagca tgacaccagt aataaggcaa tcttcgtcaa tatcaaaagt
    21121 agaggtatgc aaaggggaag taatgttttt ttcacgattt gcacaaccaa ggtggtaaaa
    21181 agctccttca cagtgtctta gaaaatacga aaagtcttca gcaccaagag tgggcgagtc
    21241 tttaaggata atattttctt caccaaacaa ttctacagca ttttgtttga cgtaatctac
    21301 tagatggttt gaattgataa gagctggata accttcctca atttcaacag aaatacttcc
    21361 tccaaatgtt ttacaagtaa aatcgcaaat ttctctaatt cgtttttttg aaaactctct
    21421 agtttcagga gtcaaggttc tcaaagtacc ccttataacc actttgtcac aaactatatt
    21481 ttctttgatt ccaccttcaa tctttcctag agaaataacc acagaatcag ttggatttgt
    21541 gtttcgacta acgattgtct gtagagctgt aataatatga gcagatataa caatagcatc
    21601 aataccttga tgtggataag ctccatgaca ttttgaacca tgtacagtta attgaagtgt
    21661 attggtggaa gcatttagag tgttgtattt taattcaatt agatttttgt tgatattggg
    21721 atttacatgg agtccaaata tatattttac atttggtttt tcaagacatc catcttctat
    21781 cataagttga gccccaccaa tagtttcctc agcaggttga aaaaagaatt ttacatttac
    21841 atttaagtaa tcctttaatt taaatagtat attacaagta cccagtaaaa tagcagtgtg
    21901 tgcatcatga ccacatgcat gcattttgcc aatattaata gatttataag aataattaag
    21961 ttcttctgtt ataggtaagg catctatatc agctcttata gcaactgttt caaagtttgg
    22021 actcacgtta atgtatgcag ttatccctgt atgattctta tattcaatat aatttatacc
    22081 tatttcattt aagtatttaa taattttttt cttagtttga aattcttcta atccaagttc
    22141 tggagtctta tgtaaatctc ttcttatatt tataacccaa tcatttatct catgacaaag
    22201 agtttttata ttaagcataa aacacatcct aactatatta aaaatttatt tttcttcatt
    22261 aattatatta ttttatgtaa atcattatta tgtatcaagt acaaataaaa tatcttataa
    22321 ttatattaga gatatttagt atggagaaga atatatcatt ttataagaag aggtgtttaa
    22381 atgttaggaa taattgcgcc atctgggcca ttaagaaata caagtttgga ggaaataaag
    22441 tttaatttag aaagttatgg ttacgaagtt aagttttctg aaagttgtag tttaaattat
    22501 aaaggttatt tagctggtaa tgatgatatt agagcaaggg atatagaaga tatgttttta
    22561 gataaagatg tagatataat tatgtgctta agaggaggtt atggaacaac aagaatattg
    22621 gataaaataa attatgatat aataaagcaa aatccaaaac catttatagg tttttctgat
    22681 ataacaggtt taaatttagc tttctataaa aattgtggtc ttttaccata tcatggaata
    22741 atggcagcaa atgtaggtaa atggggtaaa tttacctata aatcattggt aaatgcttta
    22801 gaatttaaag atgaattata tttagaaaat ccaaaagaag aaaagattta tactgtatgt
    22861 gaaggtaaag cagagggaat aataatggga ggaaatttat ccttaataat tgccacaatg
    22921 ggaacaaaat atgaaataga tgcaaaagat aaaattttgt ttattgagga aattggagaa
    22981 cctcaatata aactggatag aatgttaaca caactatatt cttcaggaaa acttgaggaa
    23041 tgtaatggta taatttttgg agattttaag gattgtattg aagaaaatga tttgatggaa
    23101 ttactaatag aatttgcaaa taaggtcaat aaaccttcta tatataatct tcagtcagga
    23161 cattgtatcc caatgataac tattcctttg ggcagaatgt gcgaattaga tgctacagaa
    23221 aaaatcgtta aattaaaaaa atagtgtgta tataaatata atagaaatat ggtataatct
    23281 tatacaagta aaaatttagc taggaggatg aaaaatgtct gatgatagaa taatagactt
    23341 taacgaacta aagaacaaag ttaaagattc tgatgtagat aaatttgagc aatacatata
    23401 taacttatat ttttctgtta tggatggaaa gatgtctatg gctgagtttt caagaaaaat
    23461 attcgattat atgagagata acaacatatc tcaagaaaaa tttatgaaaa tacaaaagca
    23521 atttatggag agatatggta tggacactga agaagtagaa aaacaactta gaaactttgg
    23581 aatcgaccct tctacagcag gttttatgag taataatact tccagcaaag tatctacaga
    23641 agatttagag tcatttaaga agagtgcagg attttatgag aaatatggag aaaaaatcca
    23701 accaaagagt tgtataacaa catttataaa aaatgactta aatgatatta atgtaataat
    23761 cgaccaagaa aagataatgc tttgtagtga tagaaaaata aatcttatgg attcagaact
    23821 aaatgagttt ttattagaat ataagaatat gtttaataaa aaaataaagg tagtaatgtg
    23881 cgaaactacg aataagtatg attattaaaa aatgaattta aatactgaag tagtataaat
    23941 taattgtact acttaaaaat aaaaatcgca atttattatt ccttataaat ttgtataagg
    24001 aatatctaaa ctgcgatttt ttatattgtg aagattagta ataatagata gtgttttaaa
    24061 ttgtaatctt ctcactaaaa ctgtgttttt ctattagaaa ttttattaaa aataaatatc
    24121 tgataattgt attatatggg atgtaaagta ttagaagctt aacgaatctc ttttaataca
    24181 ttaactccat tttgaatcat actgttgaaa aatactaagt ttaaaatctc agcagggtga
    24241 acgttaggaa tatcataagt atctactaaa ttcataggta caactatatt tttttcaata
    24301 ttatgttgat taaaatatga ttttagagtt attgcaaatt gatatataca tatatctgtg
    24361 caatcgccaa ctataattat attatctata ttatcaaaat ccaaattttc taatgcaaaa
    24421 aatccattag tagaattttt aggaagaatt tgtaggtttt ctatcgactt aagttcatca
    24481 accaattcac tttctacatc attttccaaa cagtgaacag gataactcaa aagttctatt
    24541 gatttaggag tatgagaatc agtaaatgct attactctat ttaatttgtt tgatattttt
    24601 tttgtaaaca tttcaattgg ctttattaaa gactctactc taggagagta taatgcacct
    24661 tgtcttgcaa agccattatt aacatcaatt ataaataaag cagttttact caaatcataa
    24721 ttttctattt tttctattgg tagattatct aaattactct ttaaagcttc aagttcattt
    24781 aataagttat ccatagttat acctccaaga taaatttaat aaaattgtac atattaatta
    24841 tataaaagtc aaggagtaca aactattgta taattagctg tattgttttg agtgtaaaag
    24901 atttgaagca atatctggat agttagtaaa aataccatct acattgagtt ttagacagtg
    24961 tagtatatca ccctcttcat ttacagtata tacattgaca ccaagtagat ttttgtgaca
    25021 aagttcaatt agagattcat ttactaaaac tagaggtgga tgaagataac tattacccag
    25081 aaatttagca aaattgacta cattgtttat aggtaaataa cataataatc ctgtttttat
    25141 actagggtct aatttatgaa agttttttaa actattgctg ttgaaactag atataagaac
    25201 tctatttttc atattatact ttaaaataag ctctaaaacg tctttttcta tattgggata
    25261 gtttattttg tcagttttta gttctatatt taaaataagg tctgtaggtg aaattagaat
    25321 taatacttct tctaaagttg gaattttaca ttctttattg ctttgatatc cttcaaaaga
    25381 tgagtttaga gtttttaatt ttcttaaagt aaagtctttt acaaaaccat gtccattaaa
    25441 agttctatca accttttcat catgaataac tacaattttt ccatccttag ttctgtggac
    25501 atcaagttct attccatata tatccatatc caaacatttt ttaaaagcta gaatagtatt
    25561 ttctggatac tttccactaa accccctatg agcataaata ttcatttata tggttccctc
    25621 ctataagtat aacttat
//
LOCUS       DACULQ010000038        25599 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.038, whole genome shotgun sequence.
ACCESSION   DACULQ010000038 DACULQ010000000
VERSION     DACULQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.038"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            114..1331
                     /locus_tag="JA154_06355"
     CDS             114..1331
                     /locus_tag="JA154_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HAT7995485.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1426..1806
                     /locus_tag="JA154_06360"
     CDS             1426..1806
                     /locus_tag="JA154_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089026.1"
                     /note="has endoribonuclease activity on mRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive intermediate/imine deaminase"
                     /protein_id="HAT7995486.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2090..2572
                     /locus_tag="JA154_06365"
     CDS             2090..2572
                     /locus_tag="JA154_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAT7995487.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2593..3408
                     /locus_tag="JA154_06370"
     CDS             2593..3408
                     /locus_tag="JA154_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAT7995488.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3461..5011
                     /locus_tag="JA154_06375"
     CDS             3461..5011
                     /locus_tag="JA154_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAT7995489.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGYIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSVIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5108..6460
                     /locus_tag="JA154_06380"
     CDS             5108..6460
                     /locus_tag="JA154_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HAT7995490.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6591..7349
                     /locus_tag="JA154_06385"
     CDS             6591..7349
                     /locus_tag="JA154_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7995491.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLISNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELFNLLHTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7396..8115
                     /locus_tag="JA154_06390"
     CDS             7396..8115
                     /locus_tag="JA154_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HAT7995492.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8239..9639)
                     /locus_tag="JA154_06395"
     CDS             complement(8239..9639)
                     /locus_tag="JA154_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7995493.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9643..10533)
                     /locus_tag="JA154_06400"
     CDS             complement(9643..10533)
                     /locus_tag="JA154_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7995494.1"
                     /translation="MTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKATFYKHF
                     ETKESFICEIINYYDEKFLEIIHSINENNDIPASEKLNRKIIAVWKNIFSRTTINTYI
                     RENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDALIHQFI
                     YIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYFSLHNKS
                     LFFKMIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAYLEKEDT
                     LRPKVLLLNSIKNQLKKETL"
     gene            10812..11459
                     /locus_tag="JA154_06405"
     CDS             10812..11459
                     /locus_tag="JA154_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HAT7995495.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11783..14422
                     /locus_tag="JA154_06410"
     CDS             11783..14422
                     /locus_tag="JA154_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAT7995496.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14480..15583)
                     /locus_tag="JA154_06415"
     CDS             complement(14480..15583)
                     /locus_tag="JA154_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HAT7995497.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIHLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            15944..16765
                     /locus_tag="JA154_06420"
     CDS             15944..16765
                     /locus_tag="JA154_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAT7995498.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16844..17055
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17142..18458
                     /locus_tag="JA154_06425"
     CDS             17142..18458
                     /locus_tag="JA154_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAT7995499.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18707..19045
                     /locus_tag="JA154_06430"
     CDS             18707..19045
                     /locus_tag="JA154_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HAT7995500.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19172..20461)
                     /locus_tag="JA154_06435"
     CDS             complement(19172..20461)
                     /locus_tag="JA154_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAT7995501.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20541..21245
                     /locus_tag="JA154_06440"
     CDS             20541..21245
                     /locus_tag="JA154_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HAT7995502.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQRNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21337..22383
                     /locus_tag="JA154_06445"
     CDS             21337..22383
                     /locus_tag="JA154_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21385..21387,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAT7995503.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22410..23813
                     /locus_tag="JA154_06450"
     CDS             22410..23813
                     /locus_tag="JA154_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAT7995504.1"
                     /translation="MSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREVIDL
                     KRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLGRSL
                     LSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNAAAV
                     LLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYKEAI
                     TEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKYGLS
                     YEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDKLTI
                     CALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINIEDG
                     FSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDVRTI
                     FDDEFDVIVEELRKAFN"
     gene            23927..>25599
                     /locus_tag="JA154_06455"
     CDS             23927..>25599
                     /locus_tag="JA154_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAT7995505.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKV"
BASE COUNT         9920 a         2936 c         4526 g         8217 t
ORIGIN      
        1 gtgattaatt aaaaaattat aaaaatcgtt tacatgttga tttttaaaaa aaaaagatat
       61 agaattatat agaaagtaat aaacaaaaat tggatttacg ggagtggaat gaaatgatga
      121 atgttacctt aacagatgta aaagaggcta gagagaccat taagaacata gtaaaaagaa
      181 ctgacttact tgaaagtgtt aaacttagcg aaaaaacagg agcaaatgtg ttctataaat
      241 gtgagaatct tcaaaaaaca ggttctttta aattaagagg agcttgtaac aaaatagcga
      301 gtttaacaga tgaagaaaag gcaaatggag ttatagcttc aagtgcaggg aatcatgctc
      361 aaggagttgc attgggagca aaaatgactg gtataaaatc tacaatagta atgccagcaa
      421 ctgcgccttt agcaaaagtt agtgcaacta aaggttatgg agcagaagta gttttaaatg
      481 gtgcggttta tgatgatgca tatgcaaaag ctgttgaaat acaaaaagaa acaggagcaa
      541 ctttcttaca tccattcaat gataagtatg ttatagcagg acaaggaact atatctcttg
      601 aaattttcga acaattagac aataaagttg atactatact atgcccagtt ggtggaggag
      661 gaatcatctc tggtgttgct gttgcagcaa aagcattaaa tcctaatgta aaaatagtcg
      721 gtgttcaaac tgcaaatata ccttcaatga aagagtcaat aaaaaatgga aaagtaacaa
      781 cagcatttaa tgatactaca atagctgatg gtatagctgt taaaactcca ggagatttaa
      841 cttttgaaat aataaatgaa ttggttgatg aaatagtagt ggttgaagag acagaaattg
      901 cagaatcaat attatttatg atggaaagtc aaaaaatagt ttcagaaggt gctggtgctg
      961 tatgtacagc tgctatatta agtggtaagt atgtaccagc aaaagatgaa aatgtagttt
     1021 gtataatatc tggtggtaat atagacataa atacgttata tagaataata ggtgttgctt
     1081 tagctaaaga aggaagaaga tattcattta gtacaattat ggaagataag ccaggaaact
     1141 ttgctgagtt aactagaata ataagtgaga atggtggaaa cattttaagt gctaatcaag
     1201 gtaaattatc agcaggggaa gctctaggaa aacaaagtgc ggaatttata ttagagacaa
     1261 tagattatga ccatatagct agaataaaga aagctataga agaaaaaggt tttaaaatta
     1321 tagaattata ataaaaagtg tatataatat acaagaaaaa tagtataata gtgtatgaag
     1381 atacatttta tgtgtattag attaacgaat taggaggatt ttaaaatgaa acatgaagta
     1441 atacatacta atgatgcacc agcagcttta ggaccatatt ctcaagctat aaaagctgga
     1501 aacttgttat ttgtatctgg acaagttcct cttgtaccag agactatgga agtagttgaa
     1561 ggagatgtac aagctcaaac tgctcaatct ttaaaaaatc ttaaagctat attagctgaa
     1621 agtggagctg atttttcaaa tgtagttaag actacagtat ttataaaaga tatgaatgaa
     1681 tttggggcta taaacgaagt ttatgctgaa tattttggag aaaataaacc agcaagagct
     1741 tgtgtagaag ttgctagatt accaaaagac gttaaagttg aaatagaagt aatagctgta
     1801 ttataaaaat acaatatttt taatttgaaa aggctgaaaa actatattct agtgattcag
     1861 cctttttata aaaaaataaa tcacaataaa gaacatgata aatattgtcg aattaaaaaa
     1921 taaaattaaa ataaatattg caatattttt gagaaggttt tataatgagt atataatttg
     1981 aaacgtgtaa ctattaaatt aggcatgagt tcttggatat ttttatcctg aggattcatg
     2041 ccttttttgt attgaaattc aaaaatctat ttaataagga ggaagtactg tgttaaattt
     2101 tttcaagaaa aataaaagct ataagttaca tgcagtagtg agtggtaaca gcattaatat
     2161 tgagaaagta aacgattctg tgttttcaaa aaagttaatg ggagatggag ttgcaattat
     2221 tcctaatagt aatgttgttg tagccccttg taatggtaaa gtaactgtgc ttacagaatc
     2281 aaagcatgcc tttggtatgg tctcagatga gggtgttgaa atattagtcc acataggaat
     2341 tgatacagtt agtttacagg gtgaggggtt taaaaatgaa gtgtctcaag gagatactgt
     2401 taaaaaaggt agtccaataa taagttttga aagagaaaaa ataaattctc agggaattga
     2461 ttgtacaaca ataataattg tattaaatca tagcgaattt tcagaaataa attgcatggt
     2521 agaaaatgaa gttgttgcag ggcaagatac tgttattgaa attatgaaat aatagaaagg
     2581 gattaattca atatgagagt agtaaaaatc tttaataata atgcattatc tacagtaact
     2641 aatgatagga aagaagctat tttactaggt cttggtatag gttttaataa acgtccagga
     2701 gataaagtta atgaagataa aatagaaaag atttattatg ttcaagacca catgcaaaca
     2761 aaatttttag aattattaaa aaatgttagt ccagaagtga tggatgcttc acaacaaatt
     2821 ataagtctta taccaggaaa tgagggaaaa tttaataata aagggatatt atctttagtg
     2881 gaacatatta gttttgcaat agatagaatg aaaaataatg tttttttacc taatttaatg
     2941 cttttggata taaagatgat gtattcaaaa gagtttgaat taggagtaaa agctttagaa
     3001 attatatata gagtatgtca tatccatttg cctgaagatg aagcaggtta catagcttta
     3061 cattttgtca atttacaatc aaatgataat ttggcatatg atactttaaa atttgtaaaa
     3121 ggtagtattg atttgattaa agaatgttat ggattagaat tagacgaatc gagtttaagt
     3181 acattacgtt ttagaactca tcttaaattt cttgctcaaa gaatttttaa gaatgaaatt
     3241 tgtcaggatg ataaaatgat tgaaatgtat gattatttaa ttaacaatca tcctaaaaat
     3301 aaagaatatt tagaaaagtt aaatgtatat attgaaaagg agtttaggta taaattagat
     3361 aaacctgaaa aaatttatct actactacac ctaacaaaaa tcttataaga atgagatgaa
     3421 ttagtaatag aaatatatat aattttaggg ggggataaga ttgaaagaaa aactacttgg
     3481 aggactggaa acatttgcaa aggcaatggt tcagccatta atgtatttat cgagtgcagg
     3541 aatattaatg gtggtaggtg tactacttac aaatgatatg ttattaggag tacttccatt
     3601 ttttaaatgg gagccaattc aaatatttgg aagcctaatt tataactgta taatggctat
     3661 tattaataat cttgctgtta tatttgctat aggtattcct gctgcactag caaaaaaaga
     3721 gaagcacaaa gcagcgttaa taggatttat gagttatata atatacctta cagcttccaa
     3781 cactatgtta acatcactag gtcaactagc aaaagaaagc aagatgcttg gactgatagg
     3841 gactggacaa gctactattt taggtattca agttgtagat acaagtgtat ttggtggaat
     3901 tattttaggg tatatagctg gatatgtatt taatcgtaca agcaacaaaa catttaaagg
     3961 agctttacag atatactcgg gagcaaattt tagttttata tgcatgttct ttgtatccat
     4021 tttaattgga gttgtgagta actttatatg gccattagct caacaaggaa tagcttctct
     4081 tgcaaatgtc atcaaagata caggtacatt tggtttattc ttatatggtt ttttagagag
     4141 aatattagta ccaactggac ttcatcattt aatttataca ccatttcagt tttctgattt
     4201 aggaggagtg cttaaagttg gtgcagatac agtttcagga gcctatgcta tagttatgac
     4261 agaaatgaat ttaccagttg agcgtttctc agatagtata tactacatgg caactggatt
     4321 tacaaagatg tttggttata taggtatagg agctgccttt atttatactg catataagca
     4381 aaataagaag aaggttaagg caacgattat tccactaata attacggcag tattagcctc
     4441 tgtaacagaa ccattagact ttatgtttgt attctgtgca ccacttttat tctttgtaca
     4501 tgcagttata gctggtcttt ttatagcttt attaaaaata tttgatgtta ctgcattatg
     4561 tggagggaac ttattagtat cagttataat gaatactaca gtaggcgtag aaaaaacaca
     4621 ctggccagta atgatgatat taggacttgc acaaattatt gtatattttg tagtatttag
     4681 ttttttaatt aagaaattta attataaaac tccaggtcgt gaagatgaga gtacagctac
     4741 aggcgaagaa caatcaaaac aacttaattt agatgcaggg atagaaaata taattgatgg
     4801 gttaggcgga aaagaaaata ttaatacagt agaaaattgc atcactagat tacgtgttaa
     4861 tgtaaaagat gaaagtaaga ttaatgaaga tataattaat ttaacgccaa atagcgggat
     4921 tgtaaggaaa ggaaaagata tacaaataat ttttggactt catgtgcatg aggtaagacg
     4981 tgcagtagaa gattttttag aagcttatta aagatatata tttatatagc aagggtaaat
     5041 aacagcttta agtgctgttt actatttatt aaagatacat ataatttata gaggaggaat
     5101 aattattatg attattacta ttgttggtgg aggaagcaca tttactccag gtattgttaa
     5161 gtcaattgca ttaagagaaa aagagttaga gcttgaggaa attcgtttat ttgatattga
     5221 taaaaaacgt caagataaag tagctgtagt tgtcaactgg atactaaaag aagatgttaa
     5281 aacagatgtt aaactaactg taactaattg tgaaaaagaa gcgtatacag atgctacatt
     5341 tatatttgca caaatgcgtg ttggtaaata tgaaatgcgt gagcaggatg aaaaaattcc
     5401 attgaaatat ggttgtgtag gtcaagaaac ttgtggttgt ggagggatgg catacggact
     5461 gcgtacaata caaccaatga taaaggttat tgatgatgta gagaaatatg ctaaaaaaga
     5521 ttattggata ttaaattact ctaatcctgc tgcaatcgtt tctgaagctt gtcgtaagtt
     5581 aagaccaaat gcaaggatta taaatatctg tgatatgcca atagctatta tagatatgat
     5641 agcggggtca ttaaatatca atgacgttca taatatacgc tatgattatt ttggtctaaa
     5701 tcattttggc tggtttactt caatagatta taaacataga gacttaatgg aagagattaa
     5761 ggaatacata aaagaaaata aaatattatt accaaaatct tatatagaaa acttaaataa
     5821 aagtcgtagt acaggaaatc gccatgctgt aagtagctgg atacatgttt gggaatctgt
     5881 ttatacaatg ttagaaagct tcccaaaata tctgccaaac acttatttaa catactatct
     5941 tttaagtaaa gaaacagtgg aacattcaaa cataaatcgt actcgtgcaa atgaggttat
     6001 ggaaagccgt gaaaaagact tatttgaagg tgtgagacat tacttagaaa ctggagaaat
     6061 ttctgaaaaa gcattttatg caggttcaca tggagattgg attagtgatt tggcagtttc
     6121 aattaaaaat gatacacgta gtcgtttttt agttataact gaaaatcgtg gagctatacc
     6181 aaacatgcca tatgatgcaa tggtagaaat tccagcatat attggtaaag atggtccaga
     6241 agttattgct cgaggtccaa tacctctatt tcaacaagga ttaatgatgc aacaacttaa
     6301 cagtgaaaaa ttgatagtag aagcttatat agaaaatagt tatgaaaaag ctttacaagc
     6361 attcacatta aataaaacta ttccaagtat gaatgttgct aaagaaattt tggatgaaat
     6421 gatagaagtt aataaggaat actggccaga attaagatag taggataaaa gaaagggttt
     6481 ttatttaatc attagatatg ttttcagagt cttttatcaa aatgtattca caaacatatg
     6541 gcaatatatt aaaataaaga gtgatatttt tagaagaatg gggtgatgca atgaacatta
     6601 taaataaaat tttttggcaa gaagatagga ttggcatgat tacaaataat ttagaggcag
     6661 atacacatag ccactgtatg ttacaactgt tcttagggat agaagacagc attgaaataa
     6721 cagttaatga aaaattagtt aaatgtaatt gtatcatagt agataaaaat atctctcatt
     6781 cattttcagc aagaaaaaaa gtttattact ctgctattat tgaacctaca tctatatatg
     6841 cagaacagct tacctcaaaa atgaatgatt ttggctactg gatatgtgac aatgatggat
     6901 tagagaaatt acgtcaacaa ggaacttttt tgatatctaa ctctagtaaa gaacaatatt
     6961 taagatttat ggaaatgctt aataactatt tgaatatacc tataactctc aaacattatg
     7021 atgacagaat tactgaattg ttcaatttat tacatacttg taattgtgat aatcatacta
     7081 tttcaagttt tgcagataaa gtgtctctat cagcaagtag attatcacat ctgtttaaag
     7141 aacaaatagg gataccttta aaaagctata tcttatttca tcaaatggag tgtgctttta
     7201 gagaattact ttctggaaaa aatgttaccg aagcttctat gactgctgga ttcgatactc
     7261 catcacactt tgcagggaca gttaagcgaa tgatgggaat gcctgtttct ttatctttga
     7321 aagatagcga gtttttgaaa gtatattaaa tattctcgtt atataatgaa gttgtactaa
     7381 gaaaggggct ttgttatgaa taaagaatat ttagaagaaa caaaaatgtt aaattataat
     7441 gaaccacaac ttaagctttt ggtatcatca aagaattggt tggaattaga tgattttcat
     7501 aaaataaaat ctatttatga gtttgtacag aatgatattt tatttggata taatgcttct
     7561 gatatgttaa gcgcaacaca ggtattaaat gatggtatgg gacaatgcaa tacaaaagct
     7621 actcttttaa tggcattatt acgtgcagtg aatatccctt gtaggcttca tgcttttgat
     7681 gtaacaaaag attttcaacg aggagctaca tccaaattga tttcactatt agcaccaaaa
     7741 tatattcttc atacttgggt agaggtattt tatcaagata ggtggattgc tctggagggt
     7801 gtcataactg ataaaaaata tttagaagca attcagaaga aattttttaa tcatggtgga
     7861 acgtttaaaa agtatgcaat agctacaaat gatttgaaaa atacatctat tgattgggat
     7921 ggtaaagata cttttatcca aaaagaagct atcgtgtatg attatggtat ttttccatca
     7981 ccagacgtat ttttttctac tcattcacaa catatgtcaa aattaaaaaa tttcatatat
     8041 gtacatctta tcagaaaaat aatgacaaaa aatgtatgta aggctagaaa caattatatt
     8101 gataaaaatg aatgagtgtt taagaggtaa ctatatagga aatatataga ataattaaaa
     8161 ctgtactggt taaaatgaat aaaaaggagt tatcacagag caaacttaaa ttgttcatag
     8221 gataactcct tttctgtatt aacttctaat aatttgatgt ctttttataa aacatataga
     8281 tacaattaaa ccagtaaatg caagagtagc tgctattgaa aatgcaacat ctattccatg
     8341 aatgttagac agcatgtttg aactcatatg agctttctta gtagcacttg tcattatagt
     8401 aataagtata gcagaaccta tagctccaga tatctgacgg agtgtgttat taattgctgt
     8461 ggcatgagat ataagttctc tatctaatgt tttaattccc catgtagtta atggcattaa
     8521 aaccattgaa ataccaatca tacgaataca atacatcaat gacacatgaa ttaaagatgt
     8581 atctctgcct aaaaaagcga aggataaagt tcctaaaaac aaacatcctg ttcctaatat
     8641 tgatagagca tgaggtccat atttatctaa atgacgtcct gcaattggat tgagtacaac
     8701 catcaatata gcaccaggta acattaatga acctgaagat aatgcagaaa atcctcttac
     8761 agattgaacg tacagaggaa tcattaatgt agcagacatc attgaggcat atacaatcat
     8821 aattaatatt gtagaaataa gaaatggttt atttttaaaa accctcaatt ctaaaaaagg
     8881 ttctttagat tttaattgac gcaatgtaaa taaaattaat gacataattc ctattaatat
     8941 tggtaaatat gtggcaatat tagtccaccc ataattacct tgattagtaa atccaattaa
     9001 taaaccacca aaacctagag atgatagtac tattgaaggt atatctaatt ttagtttttg
     9061 agtttcccca acatttctta ataaaataaa tgatagtata acatctaata tagcaatagg
     9121 ggaaagaaac agaaataaac tatgccaacc aaatgaatct actaaccatc ctgataaagt
     9181 tggacctatt gctggagcaa atcctactgt aataccaact aaactcatag ctgcaccacg
     9241 tttttcctca gggcataaat agagtataat aacttgtaaa agtggcatta gagaaccaga
     9301 acccattgcc tgcaacacac gagcaatcaa catagttgaa aaattattag aaaaaagtgc
     9361 aataatacat cctatagaaa agatagacat tgatgtaata aataactttc tagtactaaa
     9421 tcgatttatt aaataagctg ttgtgggtac aataatacct gcaataagta gataaattgt
     9481 tgttaaccac tgaccaacat cagcagtaat tttaaaatct gacattatat taggtaatgc
     9541 tgttgacaat agtgtttgac tgagagaact tataaaagca ccagtcatta caattgctat
     9601 tattaaattt ccttttttat tgttactttg ggtttccata atttataacg tctccttttt
     9661 taattgattt ttaatgctgt tcaataaaag tacttttggt cttaaagtgt cttctttttc
     9721 taaatatgca atcattgcat ccataattaa agaatcatat tgttgatttt ttccctcgac
     9781 gtataatttt tttattgctt ctagtaattt aggattttca aggagagaag tatcagtttt
     9841 gattagttct tctatatctt gtatcatttt aaaaaatagc gatttattat gaagggaaaa
     9901 gtaaacttca tcttcttgtt tatgtaaaaa cattgatttt tcaattaaag gatttagatt
     9961 atccatattt tctacaacta aatctagaat ttgtatagta aatttggcaa taaagtatac
    10021 atttatttct cttttttgta catgaataat gtatataaac tgatgaatta gagcatccaa
    10081 taagaaaact aaatcaaata taattttttc tattttatca ccatagttat caaataagct
    10141 taatttgtat tcattcaata agttagctcg tgattttgtt tgcaatttac ttgtagcctt
    10201 acgttgctct tcagaaaaat tttctcttat ataggtattt atggtagttc tagaaaaaat
    10261 atttttccat acagcaatta tctttctgtt taacttttct gatgctggaa tgtcattatt
    10321 ttcatttatg gaatgtataa tttctaaaaa cttttcatca taataattga ttatttcgca
    10381 gataaaactt tccttagttt caaaatgctt gtaaaaagta gctttagaca ttttacactc
    10441 ttttacaaca tccgatattg aagtattttg aaaacccttt tggtcaaaaa ttttttgtgc
    10501 aatagttata atgagccttt gtttttctgt cactttatta tctcctttca cgcaaaaacg
    10561 tttagtacta tagagtacct acaagtttta ataaatatat tatacatcat ttggaaaaaa
    10621 aatcaatata tagactgtaa tatgaaattt ttgaaaaatt tgttgttatt tttagtcgta
    10681 taaaaaagaa acttaagagt ttcttaagaa ttgtttaaag aagttgcaat aaattagtgg
    10741 tatgattata gtatgaaaga gaatgtcttg aaatttttct gtatcaaatt ccaattatgg
    10801 aggtgtagta tatgaaaaag aaaatttgtg tagcaataat ttttgctcta tttatagttt
    10861 ttgtagaaat gtttattgat agctctagcg aaggaagtaa aattatgaca gctgaattaa
    10921 gtaaatacag gaaaggcgta aatgtattat caataaacaa tattatagta gcgaataaaa
    10981 aatatagttt gcccaaagat tattctcctc aggaaagcag tgaagcgaga gatgcttttt
    11041 ataaaatgaa taaagatgct caaaagtcag gattaaattt aaaagctttt agtacatata
    11101 gaagttatga atatcaagat agattattta agtcatatgt aaaagaacat ggagaaaaag
    11161 aagcaaatag attttcagct aaacctggag agagtgagca tcaaactgga cttgcttttg
    11221 atattggagg agatgaccag tcttgttggg caaataaaaa gttcaacaat acaaaagaag
    11281 ctaagtggtt gtatgaaaat gcatataaat atggttttat acttagatat ccagaaggta
    11341 aagagaatat aacaggatat atgtatgaat cttggcacta tagatatgtt ggaactgaac
    11401 atagcaaaaa ctttgcaatg aataatttga ctttagaaga atatttacat ataaattaag
    11461 agatggtttg ttaaaattag gttaaaataa aaaataacct agactattag ctgtgtaaag
    11521 tgatataatg taaatacaat ttagataact actcgaaggg gagtagctta cacgaaagtg
    11581 gtaattaatc gtcaagacag tagaaatact cggttaatta gcaagggatt ccttgatagc
    11641 aagaccttca aaataagcat taggtatgtt tgttttgggg tcttttttta ttcaaggaat
    11701 ttttatttaa aaaaatttaa atttcaagta tacataagag cccccaaaca taaaataaat
    11761 ttattgaaga ggagggcaac aaatgtctta caaaagtact tatcaagaag taattaaaaa
    11821 tttagactca agtttatctg gtttgagtaa agaaagagca gaagagttgt tagaaaaaaa
    11881 tggagctaat gaattaaaag aagcagataa ggttcctact tataagcttt tcctagaaag
    11941 ttttaaagac ccattagtaa taattctatt gatagctgct ttagtacaaa tatttttagg
    12001 tgaaacagtg gagtctataa taatttttgc agttattatt ataaactctg ttctgggtgt
    12061 tgtacaaaca aaaaaagcag aaagttcatt agaaagtttg aaaaatttat ctgcaccaaa
    12121 cgcaaaggta ataagagaca ataaaaaaat gacaattcca gcaaaaaatc tagtagttgg
    12181 agatattgta ttcttagaag ctggcgatta cataccagca gatggaaggc ttatagaagc
    12241 acaatctctc aaggttgtag aagggatgtt aactggagaa tcagaaccag ttttaaagca
    12301 tacagaaaaa ataagtgaag atgtaagttt aggtgaccag aaaaatatgg tatttagtgg
    12361 tgctgtagtt gtttatggta gaggttcttt tgtagtgact ggaacaggta tggatactga
    12421 aatgggtaaa atagctgggc ttttagaaag tgctgaaaat aaacttacac cattgcaaca
    12481 aaaaatagag gattttagta aaaaacttgg tgtaggtatt gtaatacttg ctcttttaat
    12541 atttataatt caagtagcaa gaaattactt tatggctgga aatggagaaa tgacacaaat
    12601 tattttagat tcattcatgt tctctgtagc agtagcagta gcagctatac cagaagctct
    12661 atcatcaata gtcactatag tattggctgt tggaacaaat agtatggcaa aaaaacaagc
    12721 cataataaga aaattaccag cagtagaaac tttaggttca acaagtgtaa tatgtactga
    12781 taaaacaggt actcttacac aaaataaaat gacagtagtt gattattata tgtatggaac
    12841 taataatgat gagttagata aagaaaaagt aaactattct tatcatgcta aattattgac
    12901 tatggtttct acattgtgta atgactctca tataactagt gatggtaaag aagttggtga
    12961 cccaacagaa gtagctttaa taaattattc aagtaaaaat gatttagatt atgataaatt
    13021 aagaagtaag tatatcagaa taaatgaaat accatttgac tcagacagaa agcttatgtc
    13081 tactgtaaat agtatatatg gagattatat aatgtttaca aaaggtgctc cagatattgt
    13141 gtttagtaga tgtaaatatg ctttgaaaaa tggttcaaaa gtagatataa cagatgagat
    13201 aatagaagaa tataaacata aaaatgaaga gttttcaaat agagcactta gagtattagc
    13261 ttttgcaata aaagatgtcc cagaagaaga ttttatacct tcaattgatg acgaacatga
    13321 catgacattg gttggaataa tggcaatgat agacccacca agagaagaag taatggatgc
    13381 agtaaaagag gcaaagagtg ctggtataaa aactgttatg ataactggtg accataaaac
    13441 aacagcagca gcaattgcaa aagagattgg tataatggaa gaaggagact tggctcttac
    13501 tggaaaagag cttgatgctt tgagtgaaga acaactatat gaaaaactag aaaatataag
    13561 tgtatatgcc agagtctcgc cagaaaataa aattcgtata gtaaaagcat ggcaacataa
    13621 gaataatata acagcaatga ctggtgatgg tgttaatgat gcacctgcat taaaacaagc
    13681 agatatagga atcggtatgg gaagtggtac tgatgtagca aaagatgctt ctgctatggt
    13741 attggttgat gataactttg caagtatagt aaatgctgtt gaagttggta gaacagtata
    13801 cagtaatata ataaaatcaa ttacttatct atttgcagga aacttaggag ctatagtatc
    13861 catagtattt gcagtgtttg caggttggtc aaatccattt acagctttac aacttttgtt
    13921 tataaactta gtaaatgact ctttaccagc aattgcatta gggtttgaaa agtcagaaaa
    13981 aaatataatg aataaacctc ctagacaccc ggatgaaagt atactagcag gaggaacatt
    14041 gaaggcagtt gctataagag gttgtataat aggtgtagtt acaataattg cacaatatat
    14101 aggattacaa gtatctccag aacttggagt agcaatggca ttttcaacat tgatattagc
    14161 tagaataatg caaacattac ctgcacgttc taataatcaa acagtaataa aattaggttt
    14221 tttcagcaat aaatacgtta taggagcagt tttagtctgt cttgggcttt actctttaac
    14281 actaatacca gtgctaagag gaatattctc tataccaaca acgtttggat tgtaccagtt
    14341 ggggatttgc ctagggttag cattaatttc atcaatatta atggaactta acaaattaat
    14401 aaatagaagt gaagtaaaat aatcaaaaaa ttaaagggca tattgatttt aacaatatgc
    14461 cttttataaa tagtctaatt tattctatat tatcgattat ttggaatgct tttataactt
    14521 caggtaattc tttttcttca ggcattctaa atcttatgaa atttttatca agtccagtaa
    14581 aatcacttcc agatataact cttattttta atttagcaaa ctcttgttgt aaatctatgt
    14641 ccttgttttt atgttcaact gtcataattg atacagtatc actagtttca gctatattaa
    14701 ggtttttcca tggaattaat aattgatttt ttatatcttt tgttttttct tttaattctt
    14761 ctataaattg aacatcatct aataaatttg ctgcaatact acgagatatt tcagacactt
    14821 cgtaaggagt agaaatcttt ttaatagggc taacaagttg ttctggaaga actgcatatc
    14881 ctgctcttag tcctgctaaa ccgaatcctt ttgaaaatgt ttttaaagca attacattat
    14941 cataattatt tagaagcttg acagctgaat tttcttttgg catgtattca ccataagctt
    15001 catctaccat aactgctata tcatatttag ctgcttcctt tactatattt tcaattgatg
    15061 ataaatgtat aatttgtcct gtaggattgt ttggattgtc tatgtaaatt actttatatt
    15121 ctggatttat tttttcaata aattcttttt cattaaactt gaaattatcc tctttcttta
    15181 aaagaacata atcataagtt gcaccatgca tttttatatc tgtctcatat tctgagaact
    15241 gaggtgaata tcctaaaact ttatctccct tttcaataaa tagtctattt aaaaggtaaa
    15301 taacatgtat agaaccatct gccaaacaaa ttctgtcagt atctaatgct ataaaatctt
    15361 tccaataatc gactatatta tcttttaaag caattgaatg aggatatcct cttatcatat
    15421 caaattttaa agcattaaaa gcttctacag ctttgtttga gtaagacacg gtatttatac
    15481 cttcaccaca gtctatctcg aatacatctt ctgaatgtcc agattctatt gcataacttt
    15541 ttttcaactg catttcaata gctttgttaa tttttagttt catactttat cactccttat
    15601 ttagtcaata ataaatgtta taaaagttgc tagatttaaa atattatgaa aatatataat
    15661 agtaaaaaat gaatataaat attataatag ctcactttat ttaatatgat tatatatcat
    15721 atattgtaaa atttctagca ttataagact ataaattgaa aatattcaga aaaatactac
    15781 aagaaacgat ttattttgct cttaaaataa gttaaatgaa tgttattaat tttgtaaatt
    15841 gaaatagttt aagtataaag ttatgatata atctaataag ggtaattctt tagattatat
    15901 taaggaaata taggtataca ataaaaagtt atggagttgg attatgggat ataaattaat
    15961 tgtaacagat atggatggaa ctttattagg caataatcat aaagttacag atgaaaataa
    16021 aactgcacta caaaaagtta taaaaagcgg tattaatgta actttagcaa caggaagagc
    16081 cttcgattca gctaaatgca atgtagattt tcttaaagag gacatgccaa taatagcttg
    16141 taatggttca ttaataagag agcaaaatgg aaatataata tattcaaata agattgatac
    16201 aagaacatgt ttaaacattt tagatgtatt agataaatac gatatttact accaatgtaa
    16261 tagtatagat tctatgcttt cgaaaaaaat agaaggacgt gaagatagat taagtgtatt
    16321 tttaggctct gaaacagaag taattgtaaa agatgatttg agagaagaaa tctttaaaaa
    16381 agatatacta aaatttgtaa tcatagaaga aaaaaatcca tctattttag atgaaataag
    16441 aaaagaattg agaaaagttc aaggaataaa gataacttct tcatggccaa acaatattga
    16501 agtcatgaat gaaggtgttg ataaaggtaa tgcagttaaa attttagctg aaaaaatgaa
    16561 tatagacaga gaagatataa ttgcatttgg agacaattac aatgacatag aaatgataaa
    16621 gtttgctggt cttggggtag ctatgggaaa tgcagaagaa ttgattaaac aagaagctga
    16681 ttatgttaca gatacaaatc aagacagtgg agttgcaaag gcaatttata aattcctaga
    16741 gttaaaagaa tcacttagct cttaaaacag tatataatag gaaaaaatag gttgactatt
    16801 ttgtagatag taattataat acaaatagat aaaacataaa aatttagatt cagtgaaaaa
    16861 gagagtagat atagagaaaa tctcagcgaa ttgggggatg gtgtgagcct aatgataaca
    16921 ctttatattg aaaagaactt tggagaatac tacttgaaat tcagtatggt agtaggtaat
    16981 ctcgccttaa gagaatagag tgggtaaaca ttgtttacca actagagtgg taacacggaa
    17041 tatttcgtct cttggtcttt ttgaccaaga gattttttag tttcattgag ttacaagact
    17101 tataattaaa atacaaaaaa ttaaaatatg aggtgaaagt tatgaaatta tggggcggac
    17161 gctttagaaa agcggaaaat caacttatgg aagaatttaa taaatctttt ggctatgatt
    17221 gtgtactgta caaaaaagat atcgaaggaa gtgtagcaca tgttcatatg caagtaaaat
    17281 gtggactttt aacagaagaa gaaggaaagt ctataacaga aggtttaaaa gggatacttg
    17341 aagatgtaga aaatggaaag ttagtattgg atggagagta tgaagatata catagtttta
    17401 ctgagataaa tttaattcaa agaattggag atgttggcaa gaagcttcat acagcacgta
    17461 gtagaaatga ccaagttgca gttgatatga gattgtatgc taaagaaaaa gcaaatgatt
    17521 tggtgggttt aatatctgaa tttaaagcta ctataaaaga tgttgctgac aaaaacccag
    17581 tgatgatgcc aggctatact catcttcaaa gagctcaagt agtgacattt aagcatcact
    17641 taatggcata ttatagtatg tttgatagag atgagaaaag aataaaaaat gctatagaaa
    17701 tattagatga atcaccttta ggctgtggag ctttagcagg aactacgcat gatatagata
    17761 gaagtataac ttgtgaacaa ttaggattta aaaaagtagt agataatttt atggatggag
    17821 taagtgatag agattattta ctagagctaa tgagtgattt ttcaattata atgatgcact
    17881 taagtagatt aagtgaagag ttgatacttt ggagttctca agaatttgga tttgttgaga
    17941 tagatgattt atatacaact ggaagtagta taatgcctca gaagaaaaat ccagatggtg
    18001 cagagcttat aagaggtaag actggcagag tatatggaaa cttatttggt ctttttacag
    18061 taatgaaagg aataccatta gcatataata aagacatgca agaagataaa gaaggttttt
    18121 tcgatagtgt acatactctt gaaatgtgta tacaaataat ggatagaatg atagccacac
    18181 taaaggtaaa tgaggataaa atgaaacaag cagtaaaaaa tggtttttta aatgcaacag
    18241 aagttgcgga ttatttggtt aaaaataatg ttgcttttag agatgcacat ggtatagtag
    18301 gaagtatcgt tatatattgt gaagataata aaaaagcaat agaagattta acattagaag
    18361 aattacataa gttttcagat gcttttaagg aagatattta tgattttatc gactatgaga
    18421 gtatattaaa taagggaata aaaaagaatt taaaataatg tatttatttt taaaagctgt
    18481 aaatttgaat gttatcattt aaatttacag ctttttattg tgtaagattt tattatataa
    18541 aagtaagaaa cttaaaagta gtagtattta aaataaatac aaataaaaaa ttgttattaa
    18601 aatgataatt aatatcaata agtattgaca aaattatttc taaatactat tatatataat
    18661 ataaataata atgaaaatca atatcaaata aatgggagga aatctaatga acttattagt
    18721 gattggtggt catgaaagaa tggaaaaaga ttatatggtt atggccaaga aaaaaggtta
    18781 taagacaaag gtatatacta ccatgtcatc taaattaaat aattctatag gaagaccaga
    18841 tgcagttgtc atacttacat caactgtttc acataagatg tctagaacgg ttgagtctca
    18901 agctaaaaaa caagatatat taatcgtaag gcataaaaat agtagtaagg tagcatttaa
    18961 tgaatgtcta gatatggttg atgaatgtct gggaaattgt tcaaaatgta agcttaaaga
    19021 caaacaaaaa caaaaaaata tataggtata aaaaatacat aaacataaaa ttaaaagtac
    19081 tgagataata gttataatta ttatctcagt acttttagat ttataaggat aagtatatta
    19141 taaatttttt attataattt ccaactactt gttacttttt catatttttt ttacttctag
    19201 ctttttttct ttttttcttc tttatatttg aagctattat aaataaaact aaaagcacta
    19261 gaatacctgc aataatctta acaaaagtca ttaaaatatt atttccagta aaaaatggaa
    19321 gactgctatc aagattattt tttgcaatta agtctatact gttttctagt ttaccattat
    19381 tatatacatt taaagtacca accttagccc ctttttttat aggcaaatca attttattta
    19441 actctgtttt ggtatcataa ttgccttgag attcatcttt ttctaagaat atcctataac
    19501 tattttttgg ttcataaatc aattcatgtt gttttgtaaa caatactttt ttagtatctg
    19561 tgtatttttc tttatcaaca atagtagcac acttaaagtt atcaaatcca taatcaatca
    19621 gaatacgtga gtctaaatat aaatcagcat tagttgaatt aaatacagca acgataactc
    19681 ttctgccatc ttttacagca ctcgataaaa gacatttacc agcagcatca gtatatccag
    19741 tttttatacc atcaactata tcatatttta tatttatagg ttgacctttg tatgtaatct
    19801 tatcatgtga tgtcaaaaac ttattagtgt ttacaaagta tctctcataa ggatatgctt
    19861 ttgtagcttc aaatttaagt gacttagttt ttacaatttg tctaaaagta tcattattca
    19921 tagcttctct agctatgaga gccatatcat gagctgttgt gtagtgattt gggtctggta
    19981 atccatgagg attattaaaa tgagtattag tagctccaat tttttttgcc tcagagttca
    20041 taaggtcaat aaatttttgt acattgccac caccaacata tctagctaat acaaaagcag
    20101 catcattagc agaatgaacc aaaagagcat ctagtaactg ctttacagta aaagattccc
    20161 cattttttag atacatacta gagccatcta tttgaggtaa atctttaatt tctattactt
    20221 gatttaaatc tttaacctcc tttagaacta aacaagctgt ccatacttta gttgttgaag
    20281 ctggataaag cttagaattt ccatccttat tatatagtat tttgccagac tcatagtcca
    20341 ttaaaactgc atgtttagca actatagctg gttcattatc tgcaaaagag atattagagc
    20401 ttcctaaaaa tgaagtaaat atcaataaac agattaataa aagtgataaa tttcttttca
    20461 ttgtgagcct ccaaatttta aattcatctt ttagtatagc atatatttgt caataattaa
    20521 aagaaaggag tgattaatat atgaaaaaaa aagtaaaatt tatttcgttt tttgttttat
    20581 ttattttaat aatatcatca ataatcgttt ataaatcgct tattttcaaa gatgatattt
    20641 atgttgtaag taaaaaaaat ttacctaatg aaaaaacaca tgttgtagag aatgaaatta
    20701 aaggtgaaga taataatcac aataaagtaa ctaaagacat agataaaaat tttcaaaata
    20761 ataaaataac tatatacata agtggagcag taaatagacc tggaattgta actatagagt
    20821 cagacaagag actttatgat gctgtagaat tactaggagg aacgacaaaa gaagcagatt
    20881 taaatggagt caatctatca gtaagattag aagatgaaca acattacata atacccaaaa
    20941 taggtgaagc aacatctgtt acaagtaatg atgattccaa taaaccaaat cagaggaatg
    21001 agtcaagagg tgagcctaaa aataaatcca ataaaaatat atctaacgaa tcaaaagtaa
    21061 atataaatgt agcgactata gaagagttgg attctctacc tggggttgga gaagctacag
    21121 ccaataaaat tttacaacac agagaagaaa atggacagtt cagttcaatt gaggaaataa
    21181 aaaatgtaaa tggtattgga gataaaaagt atgaaaatat aaaagattta atttgtgtag
    21241 actaaaatat aatacaaaac ttacatatag atatgttatt ataaatatgt atgtatatgt
    21301 gtgtacatgt ggaaatttag taaaggaggc acttttatga aaaaagaaca aaaaaaactt
    21361 acagagatga caacagcagg tggttgagcc gctaaaatag ggccagaggt tctggcatca
    21421 gttttatctc aattgcctaa aaatgataat atagaaaatt tattagtagg actagataca
    21481 gcagatgatg ctgcagttta taaattaaat gatgatatgg cacttatcca aactttagac
    21541 ttctttacac caatggtaga tgacccatac gtatttggtc aaattgctgc atcaaattca
    21601 ctatcagatg tatatgctat gggaggcaaa cctttggttg ctatgaatat tgtttgtttt
    21661 ccatcatgcc atgatatgga tgtacttgca gaaatattaa aaggtgggtt tgataaagtg
    21721 aaagaaagtg gagcactttt ggttggaggg catactgtag atgacaaaga gcctaaatat
    21781 ggtctttcag tttctgggat tgtaagtcca aataaagttt tatcaaatgc tactgcaaaa
    21841 cctggtgata agttaattat aacaaaacca attggagtgg gtgtattaaa tacagccatg
    21901 aaagaaggta tggtagaaca acatatagca gataaagtaa tagaaataat gatacattta
    21961 aataaatatg cagcaatgag ttttgagaaa tttgatgtaa attcggttac agatataact
    22021 ggttttggtc tacttggtca tactttagaa atggccaaag cttctgaagt aagtattgaa
    22081 attgaatcaa aacatgtacc aattattgaa ggtgcaatag aaatggcaca aatgggaatt
    22141 atacctgctg gtatgtataa aaatatgcat tatgtatcta aagatgtaga agttgtagga
    22201 aatatagaag ttgcagttca agatatttta tacgacccac aaacatcagg agggttgtta
    22261 atttcagtaa aagaagaact tgcagaggaa cttgtaaaag atatgaaatt aaatggagca
    22321 atagaagcta aaattatagg ttctgtagtt ccaaaagggg aaaaatatat tacagtttta
    22381 taaaatatta tagagaaaga gtgattgttt tgagcaaaag agaattgttt gcaatgttgc
    22441 cttcagttga tgaagtatta agtgataata gaattgtaga aataataaat gaatatccta
    22501 gaagtttagt tttagaatct gtgagagaag taatagattt aaaaagacaa tttatattaa
    22561 gattaaaaga agatgcatca aactctgtaa caatagagtt tggtgaaata atagaatctg
    22621 caatagagag agttaagtta aattactcat tatctttaaa aaaagttata aatgcaactg
    22681 gtacagttat tcatacaaat ttaggaaggt ctttacttag tgaagatata aaagatgaat
    22741 tatggtgtgc tgcttcaagg tattcaaact tagaatacga tttggataat ggagaaagag
    22801 gttcaagata ctctcattta actagtacaa taaaaagact tactggagca gaagatgtac
    22861 tagttgtaaa taataatgca gcggcagtac ttcttgtact tagcacaatg gctaaaggag
    22921 gagaagcaat tgtatctcgt ggagaattag ttgaagtagg tggttcattt aggattccaa
    22981 gtataatggc tcttagtggt gcagaattag ttgaagttgg ttcaactaat aaaactcatt
    23041 tgaaagatta caaggaggcc attacagaag atacaaatgt attgatgaag gttcatacaa
    23101 gtaattatag aataatggga ttcacagaga gtgtttctat agaagaattg gttaatttag
    23161 gtaaaaaata taaactacct gtaatagaag acttaggaag tggagttttt atagatttat
    23221 caaaatatgg tttatcttat gaaccaactg tattggattc tattcgacaa ggtgcagatg
    23281 tagtaacttt tagtggagat aagatgcttg gaggtccgca agcaggtatt atagttggga
    23341 aaaaagagta tatagaaaaa atgaagaaaa atcaacttac tagagcactt agagttgata
    23401 agcttacaat atgtgctctt gaagcaacat tgagaatgta tttagatgag acaaaagcta
    23461 tagaaaatat acctacttta aaaatgttga catataaaat tgaagaatta gaagtaaaag
    23521 caaataaact atttgaaaaa ataactgctt taaacttaaa tgcaaacata aatatagaag
    23581 atggattttc acaggtggga ggagggtcta tgcctcttga aactataagt acaaaggtaa
    23641 tatcaataac tcctgaacat atgaatgttt catctttaga aaaaaaacta agacttagtg
    23701 aagcacatat aatagccaga gtgtatgata ataaatatgt actagatgta agaactatat
    23761 ttgatgatga atttgatgta atagttgagg aattgagaaa agcatttaat taaataaatt
    23821 tgagttaata aatattaaat ttagaagtat aagtatgaag ttttgattaa aaaatagcta
    23881 cacacacttg tgactttagt catagcaggt ttagaggggg caaaaaatga aaaatgtagt
    23941 aattggaaca gcaggtcata ttgaccacgg aaaaactact cttataaaag cattgactgg
    24001 aagagagact gatacgttag atgaagaaaa aaagagaggt atttcaataa acttaggatt
    24061 tacttatttt gatttaccaa acaatagaag agctggaatt gttgatgtcc caggacacga
    24121 aaaatttata aaaaacatgt tagctggagc atcaggaatt gatatagttt tacttgttgt
    24181 agcagcagat gaaggggtta tgccacagac agttgagcat ttagatattc taagcttttt
    24241 aaatatcaaa aatggaatag tggttctaac taagtctgat actgtagatg atgaatttag
    24301 agagctagta aaagaagata tttgtgaaaa aataaaagga acttttcttg aagatgctga
    24361 aataattgaa gttgattcta tatctaaaag aggtatagac actcttattg aaaaaataga
    24421 taaaatgagt gatgatatag aagataaaaa tgaaaactct ccagcaagac ttaatataga
    24481 cagagtattt tcaataaaag ggtttggtac tgtaataaca ggcactttaa tagaaggaaa
    24541 gataagtata gaagatgatt tggttatata tccacaggta ttaaaaacaa aaataagaag
    24601 tatacaagtt catggagaaa ataaagatac agcttatgca ggtcaaagaa cagcaatcaa
    24661 tatatcaaat gtaaaagtgg atgatataaa aagaggagat gttttagctg caccaaactc
    24721 attggaggaa tcaatgatgt tagatgtaaa attatcattg gtaaatcatt tagataaagg
    24781 attaaagcac tgggatagat taagattgta tcatggaact agagagatac tttgtagagc
    24841 agttccacta gataaagaat taattgaaag tggagaaagt ggctatgttc agttgagact
    24901 tgaagaaagc atagtatcta agaaaggtga tacgtttgta gtgagacgtt attctccaat
    24961 ggaaactata ggtggaggtg taataattga cccatctcct aaaaaacata agaaatttga
    25021 tgaaaaagtt atagaagcat taaaaataaa agaaaaaggc gaactaaaag atataataga
    25081 agagtactta aaaaggaatt taaaaaatta tccaaatata aaagaaataa tgagctatag
    25141 tggtgctcat gaagaagatg taaaaagagc gcttgaaaca cttatatcag aagataaagt
    25201 atttattata ggaaatatgt atatgcatat aaatcaatac aataaattaa aagaaaatac
    25261 aataaagctt ttaagtgagt accataagaa gtataggctt agaaaaggga tactaaaaga
    25321 agaagtaagg tctaagatag agagtaattt taagactagg gagatggata ttttattaga
    25381 aaaattgtct acagaaaatg ctataaaaat agaaaataat atagtatctc tattagattt
    25441 tgaggttatt ttaaatgata agcaaaaaga aatagcaaaa aaaatagaaa aaagattaaa
    25501 atcatgtgga gtttcttcta tactgacaat agatgaagtt tctgaaggaa atcataacta
    25561 tgctgaagta ttggagtcaa tgataggtaa taaagtaga
//
LOCUS       DACULQ010000039        25076 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.039, whole genome shotgun sequence.
ACCESSION   DACULQ010000039 DACULQ010000000
VERSION     DACULQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.039"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            56..1912
                     /locus_tag="JA154_06460"
     CDS             56..1912
                     /locus_tag="JA154_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HAT7995506.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1995..2235
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2301..2536
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2621..3838
                     /locus_tag="JA154_06465"
     CDS             2621..3838
                     /locus_tag="JA154_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HAT7995507.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3955..4449
                     /gene="cdeM"
                     /locus_tag="JA154_06470"
     CDS             3955..4449
                     /gene="cdeM"
                     /locus_tag="JA154_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HAT7995508.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGENRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            4714..5979
                     /locus_tag="JA154_06475"
     CDS             4714..5979
                     /locus_tag="JA154_06475"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088082.1"
                     /note="catalyzes the oxidation of L-histidinol to
                     L-histidinaldehyde and then to L-histidine in histidine
                     biosynthesis; functions as a dimer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAT7995509.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6182..6934)
                     /locus_tag="JA154_06480"
     CDS             complement(6182..6934)
                     /locus_tag="JA154_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7995510.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7052..7321)
                     /locus_tag="JA154_06485"
     CDS             complement(7052..7321)
                     /locus_tag="JA154_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995511.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7796..8890
                     /locus_tag="JA154_06490"
     CDS             7796..8890
                     /locus_tag="JA154_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAT7995512.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISNITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8892..9842
                     /locus_tag="JA154_06495"
     CDS             8892..9842
                     /locus_tag="JA154_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAT7995513.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKVDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9835..11019
                     /locus_tag="JA154_06500"
     CDS             9835..11019
                     /locus_tag="JA154_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995514.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISNYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            11032..12528
                     /locus_tag="JA154_06505"
     CDS             11032..12528
                     /locus_tag="JA154_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAT7995515.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEECMKICEDFDFNIDPHMVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVND
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12551..13501
                     /locus_tag="JA154_06510"
     CDS             12551..13501
                     /locus_tag="JA154_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAT7995516.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13522..14484
                     /locus_tag="JA154_06515"
     CDS             13522..14484
                     /locus_tag="JA154_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAT7995517.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14856..15488
                     /locus_tag="JA154_06520"
     CDS             14856..15488
                     /locus_tag="JA154_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995518.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15914..15991
                     /locus_tag="JA154_06525"
     CDS             15914..15991
                     /locus_tag="JA154_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020062503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAT7995519.1"
                     /translation="MWFLAVVIIMLIIYLVYALINPEKF"
     gene            16020..17714
                     /locus_tag="JA154_06530"
     CDS             16020..17714
                     /locus_tag="JA154_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAT7995520.1"
                     /translation="MGGTIFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFD
                     KVDNFIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSM
                     EQGLSFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGL
                     VGKKKTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDI
                     ARGPVAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGH
                     MIKNKKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGI
                     AQSSLFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAI
                     IAVFLCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGIS
                     NPGFHGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAIS
                     SLLASKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17739..19796
                     /locus_tag="JA154_06535"
     CDS             17739..19796
                     /locus_tag="JA154_06535"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088093.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HAT7995521.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            19815..20441
                     /locus_tag="JA154_06540"
     CDS             19815..20441
                     /locus_tag="JA154_06540"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088094.1"
                     /note="one of the components of the high-affinity
                     ATP-driven potassium transport (or KDP)system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; the C subunit may be
                     involved in assembly of the KDP complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HAT7995522.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVEDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20678..21586
                     /locus_tag="JA154_06545"
     CDS             20678..21586
                     /locus_tag="JA154_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAT7995523.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21613..22203
                     /locus_tag="JA154_06550"
     CDS             21613..22203
                     /locus_tag="JA154_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAT7995524.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22363..22551
                     /locus_tag="JA154_06555"
     CDS             22363..22551
                     /locus_tag="JA154_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAT7995525.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22605..23474)
                     /locus_tag="JA154_06560"
     CDS             complement(22605..23474)
                     /locus_tag="JA154_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminopeptidase"
                     /protein_id="HAT7995526.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDVINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23671..24921)
                     /locus_tag="JA154_06565"
     CDS             complement(23671..24921)
                     /locus_tag="JA154_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995527.1"
                     /translation="MIMENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFY
                     KKEKDFYCIALAPCNVSSEKANRKISIYNMIKEINILIESITDKNELLSEELFTNNLS
                     EFYDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNS
                     LSLVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDS
                     SIDIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEP
                     EFSDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETA
                     HAIDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDE
                     DSKNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9640 a         2683 c         4531 g         8222 t
ORIGIN      
        1 tataattaat taagtagtat tattataaat aacattgaaa ggtagtggtt agaatatgga
       61 tacccataat aaatatgtaa attttataaa aaatatacct gtaccttttt tatattgtag
      121 aattgtaaag agacaagaag atatagaata tcgagtagaa tatataagta agggtatggg
      181 caaagtatta caattagaag aaggtatttg tgataaaaat atactagatg tattgcctgt
      241 atttaagtct aaaaaatatt tcaaagagtt gttctcaaat gaagtggatt gtataaaaag
      301 gtacattccg acacttaaaa actggataaa tataaaaaaa cagataatag gggattcata
      361 cataattttg tattttggta aaattgtatt tgattataga cagataatcg attcctttga
      421 taaaaaggaa aaagtagctt atattaaaga cgaagaaggc atatatatag attgtagtga
      481 aaatttaata ccaatattaa ataacaacat taaaacaacg aaagatatct ttggtaaaaa
      541 tgatatagaa gtatggggag agaatacagg aaaattattt agagatgatt atagagaagg
      601 agtatctagt aaaaaaagat ttttacaaaa tctttttgaa tatgaagaaa cattttttat
      661 ggttgaaaaa tattttttgt atgatgaaga cgaactttta ggtactatag ggatagtaga
      721 caatataata tatagtggtt acagtaatag aaattataac tcaaaagatt taatgaagat
      781 gatagaacac tcaattccag agaatatgtt ttataaagat gtgtatggaa attatattgg
      841 atttaattca ggttttttga atttagcttg tatgaataaa gaagaattat tagggaagaa
      901 tagttataaa atatcagaag aagaagcttt aatagataag atatttgaaa gtgataaggg
      961 tgtagttgaa aataaaaaag tagttacatt tgagttaaat ataagtatga atgatgaaaa
     1021 caagtgtatt gagattacaa aaaggccgtt ttttgatagc tatggaagtg ttattggaat
     1081 aattggaaca gcaagggata taagtagaag aaaaagatta gaagaggaaa tggataaaac
     1141 taggatggaa ttttttgcaa atttatccca tgaacttaga acgcctataa atttaatatc
     1201 atcttcatta caagtgatag aaaaaaaaga agcagattta atagaatcaa atgatacttt
     1261 aaaaagaaat ctaggtataa taaaacaaaa tggaaataga atattgaggt tagtaaataa
     1321 tgttatagac tttacaaaaa tgcaatcagg atatttagat tttaaaccag aagaatctga
     1381 tataattgca tttatagagg agatatgtat gtctgtagct gattttgcaa gccaaaataa
     1441 tatacaactt acatttgata cagaaataga agaattttca atgttatttg attcagaaaa
     1501 attggaaaga ataatattaa atttactttc taatggaatt aagtacaata agaaagatgg
     1561 aaaaataaat atatttttat atgtgaaaga taatgtattt aatatgaaaa tatctgatag
     1621 tggtataggt ataccaaaag aaaaaataga taaaatattt aataggtttg aacagattga
     1681 caatgaactt tcttacagag ttaaaggaag tggtattgga ctttcattag tgaaatcttt
     1741 ggttgagttg catgagggaa gtatatcttt aaaaagtcaa cttggtattg gaagtgaatt
     1801 tatagtttca ttgcctgtta gaagtaaaaa taatattgaa aaatataatc ataaaagaga
     1861 aatcagcaat gaactaagta aaaaattaga aatagagttc tcggatttat aagtttaaat
     1921 ataaaaaata aaattttaaa aaagtattga caattgattt agaagggttt aaaataaatt
     1981 acaacataat aaataaaaag gcgatgaata ggaagagtaa atctaattcg attaccagag
     2041 agaagagtcc gttggctgga agacttttta aattagaggg attgaaaact acctaggagc
     2101 tactttctga agtcatctgc attagtagat tttagtaaga atagtcgtta gctgcgttaa
     2161 agtgtaaagt gagttttcat gacatatgaa aactaaatta ggtggaaccg cgggaataat
     2221 actctcgtcc ttatgttata aggatgagag ttttttgtta tttaaaaata taaacagtaa
     2281 tcataataat tatagaaaat ataaaagcaa tgaataggaa gagtaaattt aattcgacta
     2341 tcagagagga aagttcacag gctgaaagac tttctaagtt tgagaaattg aaaactacct
     2401 aggagctgtg gtctgaagtt actttaaaaa gttttagtaa gactaaccgt taactgcgtt
     2461 aaagtgtaaa gtggatttta tcaataaatg ataaaatcaa attgggtgga accgcgagta
     2521 tagactcgtc cctttatata gggatgagtc tatttttatt gcaataaaag attgaaaata
     2581 aaaaaaaatc ggaaaattat aaatttagga ggaaaataaa atggagaata aagtgaaaat
     2641 tggagtacta ggatatggag ttgtaggaag tggacttata gatataatag ataataataa
     2701 agaaaaaaga aatatagaaa tagttggaat tttagttaat aatttggaaa aacataagga
     2761 taaaaaatat tctaatataa taactaataa tatagatgat atatttaata aagatataga
     2821 tatacttgtt gaagtcatgg gtggtttaga accatcatta tcttatataa aaaaagcatt
     2881 aaacaataaa attcatgttg ttacagcaaa taaagatttg ttagctgaat gtggagatga
     2941 gcttgcaaaa ttagcaagtg aaaataaagt aagtattaaa tttgaagctt cagttgcagg
     3001 aggtatacca gtattaaaac caataataga atcactagaa ggaaataata tagatagtat
     3061 aaatgcaatt ttaaatggaa ctactaattt tatattatct aaaatgtatg atgagaatct
     3121 atcttatgat atggcattaa gacaagctca agaattaggt tttgctgaag caaatccaga
     3181 gtcagatgta ttagggtatg atgctgctag aaaactatca atattatcaa ctctagctta
     3241 tgacaataga gtatattgga aggatgtgta tttagaagga attacagata tagatgaaaa
     3301 agatattgaa tatgcaaaaa aattaaattg taaaataaaa ttgattggtc aaagtaagta
     3361 tgaaaatgat aaagtaagtg cttttgttag acctgtttta gtagaaaaag ataatatcct
     3421 tgctagaata gacaatgagt tcaatgctgt catagtgaat ggagattcag taggagaagt
     3481 ttcttttgta ggtaaaggcg ctggaagttt agcaacagga agtgcggttt attcagatgt
     3541 tatagatata atagataata gagtttcaag tatagattct tttactaaag ataaaataca
     3601 agttaataaa atagttagag aaaagtgtgg agcacttctt agatttaaaa aatgtaataa
     3661 agatgaaatt ctaaatatag ttgagaattg tttagtaaaa ttcgatattt taaatgatga
     3721 tgatgaactt gctattatgg tttatgctga ttctgaatat gaaataaaca acagtttatg
     3781 tctaataaaa gataaaggat actgcgaaaa aatgaataaa atgttgaaaa taagttaaaa
     3841 aagaaaaaat ataagtttat atgtaacata ttttgacgat tttaatacta tatagtatga
     3901 gatgaaacaa aacatctcat aaaaaaatta aaattcaagg gagagatatg ttatatggaa
     3961 aataaaaaat atgcaaatgg tggttattca gaagactggt atgaaagagg agaatctaca
     4021 gctaaatggt tccagaatga tagagaagaa tatgaaagag cagcatacga tgaagataga
     4081 gaaagaagag gctcaaactg tggatgttca gattcaggag aaaatagacc tagaaactgt
     4141 gaaagattta gaagagaagc agagataaga gaaagagaag caagagaagc attctgtgaa
     4201 tcttcagaga gaaagaaaga agcactagca tatgaatgtg aagctagaaa attatgggaa
     4261 gaagcagaaa aatactggga tgaatattca aaatacaact ataaaggaat cgaatattta
     4321 gcagaagctg ctagattatt tgatgaaggt atggaatgtg aagctagaag aaatggaaat
     4381 aatggaggaa acaataataa ttgttgccat aaatgccata aatgtaattg taactgctgt
     4441 agaaaataaa aaaattaatt gacaaatcaa tagttaaaat atatcatatt atgtataata
     4501 aagagaaaat tcagaaaaaa ataatgatta gaagaaatat aagtatattt attagacaaa
     4561 tagtgctcag ttagtgaggt gagtacaata tgattaaact cccatcctct agcttttgat
     4621 agagggtggg agtttttatt tatttatata tcttaaatga ggataatatt gtaaaataaa
     4681 tgagtatgag attgtaaaat taggaggtaa aaaatgaaga gtattaaaac atcaagtatg
     4741 aaagaaaagg tagatgaaaa gatattaaga gagcaagtaa gttctataat agaagatata
     4801 agaaataata aggatgttgc acttaaaaaa tataatgaga agtttgatag aaatacaaga
     4861 gatgaattta gaattactaa ggaagaaata aaggaagctt ataagcatgt agatgacgaa
     4921 tttataaata acttaaagat tgctgccaaa aatataagag aatttgctaa agctcaaaaa
     4981 ctaagttttg aaaacccttt tgaaaaagaa atttatccag gtgtaatctt aggtcaaact
     5041 aatattccaa tagagtcatg tcttgcatat gttccaggag gtcagtatcc attgttttca
     5101 acagctctta tgcttataat acctgcaaaa gtagcaggag taaaaagaat agtggcatgt
     5161 tcaccaacta tgaaaaatac tgaaaagata aatcctaaga cattggtagc tatggatata
     5221 gcaggtgctg atgaaatata tgcaactgga ggagttcaag ctatagcagc atttacatat
     5281 ggaacagaaa agattaatcc agttgacata attgtaggac caggaaataa gtttgtaact
     5341 gaagctaaaa gacaatgtta tggacaagta gggattgatt ttgtcgctgg gccaagtgaa
     5401 gttcttataa tagcggatga aacttcaaat ccagtttata ttgcagctga tttattagca
     5461 caatgtgagc atgatttaaa tgcaagagga atattattga caaatagctt ggaagtagca
     5521 caaaaagttg aaaaaaatat agaaagtatg ctaaaagatt tgccaacaaa ggacatagca
     5581 tatagttcat gggaaaataa tggagagata atattggttg atgatatgga agaagccatt
     5641 aaaataagta atttttatgc accagaacat ttagaagttg cagtaaatga gtataatgat
     5701 atttgtgata gattaactaa ctatggttct ttatttattg gaaatctttc tgctgaggtg
     5761 tttggtgact atgtttcagg taccaatcat acattaccaa cacttaaagc atcaagatat
     5821 actggtggtg tttgggttgg aacttttatc aaaacttgta caaagcagat atttaatgaa
     5881 aaagctatac aatcattagc acctgtagct gaaaagctgg caaaagaaga gggattatat
     5941 gcacatgcaa aggctgctga ggttaggttt aaaaagtagt aaaaaatttt attttggcag
     6001 tcgagattta gtgttagact gagataagga aaacgctgag aacccgaata aatactatat
     6061 tttgtgggcc tcggcgtttc ttttatatct gtatataata ggataatact acctgtgtgt
     6121 ttaaatgcta gcttaatatt tcttttctaa ataatgttta tgaaatagtt aatattttta
     6181 gttaatactt gaaaaaaact tttgctaact gtgcaaattc atctgcattc gtcaatatag
     6241 cagggtgtcc tccctgacta aaaatgtgaa tacttgcttt tgaaataaaa gttgccattt
     6301 gtttgtactc ttcttcaaga ttatgacgac acatttcgtc ttccttactt cctgtaaata
     6361 aaattggcat ttctaattta cacaattctt tatgaaacaa agggcgtttc tcgtttgcac
     6421 attgcaatag agcttttgta tcaagattaa caacattctc ccaatctttt ccatgacacc
     6481 attcatagaa ttttcgtgct tgaatatctt gctttgattt ttcccttcca gtgattagat
     6541 tatctatgaa gttatcatta agagtacgtc catcaaagct atctgctata attgcttcaa
     6601 ctaaatcggg acgttctaat ccagtattaa cagctgccca tgctcctcca cttgttccaa
     6661 ttaagccaac tttaccacaa ttcaaatgtt caatcaatgc tattgtctgt aaggcttcat
     6721 catgccatat gtcaggagaa aattgagaaa ctctatccga ctgtccatta cctaaaaaat
     6781 caattaagat acatctaaaa ttttctacat ataatggcac aagtgcttta aacatattag
     6841 aagaaccagt attaccatgc aggaaaatta tcggtttacc ttgtccatat tctttataaa
     6901 aacaagaatt accttgatac tgaaaatatg acatatattt tcctcctttt tatttgttaa
     6961 gttaattata acaatatagg tatgaatgaa aaacctcatg ttctttctta gaataaaaat
     7021 gtgccacgca taaaacgtga cacatttcta attataaatt ttgctgttta agtattcttt
     7081 gtatgctttt ctctgtcaaa aagtatttct ctgataactg cttgatactc attccatttt
     7141 ggaaatttat acagatttga gaattacgag aagctaagac ttgtttggtg tttgtgtttt
     7201 caccccaatg ctttttgtgc tcttgctttt tgggaatata aatcacttta ccatctatat
     7261 attcttgcac ttgctcaatc agttctttag gcagtaagtc aatcgaattt tgatagctca
     7321 ttttgcacct cctaaataat tattctttag gattagcaaa ggctactgcc actcacaatt
     7381 aaaaataaaa ttttttatta tgtacaatag cctttgctat gcacaagttc caattcccat
     7441 aaactaaata ctcctttctg gtttgaaaaa ttcatctgaa cattgatttc gcttttaaga
     7501 tagagttcaa tgacaaattt acatttttat tatatatatt cagatagata attgcaactg
     7561 tctttgtatg aggatatttt attatagtca atactataat atgtatttat atcatcaaac
     7621 taatatgaac agttgaaagg aatatgaaca ataaaactta gtcaggaaga ataattatga
     7681 agcagacaat agagaaattt gattttaaag ttttattgaa aatttattga caaatttaac
     7741 ttgtaaatat tatactaaac ttaataaata aagtttataa agtaaggtgt tgtctatgaa
     7801 aaatatagtt ggaaaaccgc aaacgataaa aaaagtaaat gaagatttaa taaaaaatat
     7861 tataaaaaat gaaggtccta taacaaaacc agaaatttca aacattactg aattgagttt
     7921 agctacagtt aataagattg tagagcaatt agctttgaaa aatgaagtta aagtaagtgg
     7981 actaagtgaa tctactggag gaagaagagc tcaattattt gaaataaatg caaacttaga
     8041 acacataata gctttatatt attatagaaa ttgttgtata ggggtagttg ccaatttatt
     8101 aggtgaaata atttatcaag aagaatttaa tataagaatg gatacatatg aaaacgttaa
     8161 ccaagatact ttttttgtta tagatactct aataaaaaag gaaaaaggaa acaatataag
     8221 ggctattgga attggtgtcc caggagttgt aaaaaaaggt gtagttaaaa atataccaac
     8281 tataaaaagt tgggaaggta taaatttaga aaatttaata aagagtaaat acaatactaa
     8341 agtgtttata gaaaatgata caaatattac tactattggg atttatcaaa aacagtataa
     8401 agacaaatat aaaaatatgg ctcttatgta tttagaagat ggtattggtt caggtatagt
     8461 tataaatggt gaattgtatt taggttctac aaattttgct ggagagttaa gttatctaaa
     8521 tatagaattt ggtaagtata actctaacaa cttaaatctt gaagattata ttatgagttt
     8581 aagaaaaaga tatttagaaa gtaaagataa taaatttaag aaagaaattt tatctataat
     8641 atcaaacttt ataaggactc ttgtatgtgt cctaaatcca caagctatga ttatacagtg
     8701 tagtatatta accaagagtg acatggagtt tatagaaaaa gatgtaagca ataatgttgg
     8761 agaagaaaat tttcctaaat taataaaagt agagtcattg agagaaggaa gtataaatgg
     8821 agtaattagt atgtgtttaa aagaaactga ctatcaatat tccttatcta ataaaaaagg
     8881 aggatattaa tgtgaaatat ttggtttcaa tagatggagg gggaacaaaa actaaatttt
     8941 gtgtaagtga cttagatgga aacatactta aagaacatac cactggttca actaattata
     9001 agtcagttgg aattaaaaaa acatatgaaa atattaataa tggatttaaa aaaatattaa
     9061 aagatttata tatcgattat gacgatattg agtacactgt atttggtata tctggttgtg
     9121 attctcctaa tgattataag attataatgg atgaaatatt aaaaattggg attaacaaag
     9181 aaaaaatata tctggcaaat gatgcagttt tagcttttta tgcacaagcg gattcccctg
     9241 gtttagtaat tgtggctggg acaggttcaa ttatactggg aattaaagaa gatggagaaa
     9301 tatatagagt tggtggatgg gggtataatt ttagtgattt gggttctgga tatgatatag
     9361 ggagaaaatt attaaagaaa gtattgcttt attgtgatga atgtcatgag tatagtgatt
     9421 tatttagttg tgtcttggat ttctttggag caaatagttt tgaacaactt gcatatatga
     9481 ttactgatat aaacaataat gttgaaatag caaacttagc ttcattagtg attgattgtg
     9541 ccgaacaagg tgataaattg gctattgaaa tattaagaga atcatcaact gaactatcta
     9601 aattagctca agtaatattg agaaaaatat ctaatccaat gaaagtggat aaaagcgaaa
     9661 taaatatagt tttgtctggt ggtacattaa ataaaactgt atatgctcaa atgttaatga
     9721 ataatttaaa aacagaaaat acagataaaa atttaaaatt tatatctcaa gaaaatcagc
     9781 cagtgtatgg ggggataaga cttgctatgg cattagctaa taggagggtc aagcatggat
     9841 agaaaaaagg taatagtaca aatttttact ggaggtttta aggctaaaga aataaagttt
     9901 aatgatataa aatgtaaact actagaatta ttggactgta ttgatataga aaaggttatc
     9961 atgggatgga gtgtagatga agaattatat gtagaaacta aaaaactatt aaataaatat
    10021 aatgttgaac tatatctttg gttgccatgt ttttctgaaa taggattact tgaaaaaagt
    10081 aatttgctaa ttgattatat ggggaaggaa gtcaagggct atgctttaca agaggatgaa
    10141 aattttgagt tttattgccc aaataataag gaaaatataa aaaatataaa gaaaatatat
    10201 agtacttatt ttagtgatat taaatttgat ggaatttttt tagataaaat aaggtatggt
    10261 tctttttcga ataaactaaa tggtgttttt aattgttttt gtaaagattg tttaaatata
    10321 tatgaaaaaa ataatattga taaagagtcg ttaatgagag aaatgaataa tgtgtctaag
    10381 ggagttatag ataatgaaga tactccattt ggtataacag aatacattaa tggaaaatac
    10441 aaatttaaaa ataaaatatg ggaagatttt tttgaaacaa aaaatcaaaa tatatacaag
    10501 gcattattag agataagtaa ttattttaaa agtaagggat tgaaaatagg tatagatact
    10561 ttttctcctt ttatttctta ctttacaggt caagatttag aaaaattaca agaagtagca
    10621 gattttataa aaccaatgat gtatagaact acaaaagctc cagctggttt accatttgaa
    10681 tttgaaaggt ttattgatga atccatacat aagaataaag agattggaaa aagaaatttc
    10741 ttagatattt taggaattaa ggagtatgaa ggagatgtat atcctataga atttgtacta
    10801 gaggaaatcg attttatgac aagaaatctt aataaagata tatatgtagg agttgaaata
    10861 aataaaaagg aaattatatc tcctgttaca cctaagtaca taagtgaaaa tatttcaaat
    10921 ataaataaga caacagtaaa aggatatgtt ttatcttggg atttgttgtc agcaccaaaa
    10981 gaaaatattg atgaaattat aaaatggttt cggaattagt gaggtgataa agtgagtaag
    11041 atattggaaa tgcaacaaat atataaaagt tttggaaagg tagatgtttt aaaagggata
    11101 aattttgagt tagaaaaggg agaagtaaga gctcttttgg gagctaatgg agcaggaaaa
    11161 tctacactta ttaaaatttt aggaggagtt cattctcaaa ctaaaggtaa agtaattttg
    11221 actggtaatg aaataatgtt taaaaatcca catcattcta aagaaaatgg tattagtata
    11281 atttatcaag aattaagctt gataccaacg cttacagtac ttgaaaatat ttttcttggt
    11341 agagaaattt taaaaggaag tgtgttttta aacaagaaag acatgcttga ggaatgtatg
    11401 aaaatttgtg aggattttga ttttaatata gacccacata tggtagtttc taatttaagt
    11461 gtagcaaacc aacaaatggt tgagataatg aaagctgttt catgtaattc tgaaattatc
    11521 ataatggatg aacctacaac atcacttact aataatgaaa aagatgggct atttaaaata
    11581 ataagtagac taaagtctaa aggtaaaaca atagtctata tttctcacat attagaggaa
    11641 gtttttaaag tatgtgatag tgcaagtata atgaaaaatg gaattatgat tggaacttat
    11701 gatgtagagg atttaactaa attaaagata actcaactta tgacaggaaa tgataatcac
    11761 aaacttattg agaagaagga ttatacattt gcaaaatatg aagagccacc agtactagaa
    11821 gtaaagaata taagctggtc taacaaaata aaaaatgttt catttaaagt ttatagaggc
    11881 gaagttgtag gtcttgcagg tcttgtaggt tcaagaagaa ctgaaattat aaatttgata
    11941 tttggtatag ataataaaga tagtggagac atttttatca atggtaaaaa agtagacata
    12001 acttctccta aggttgctat aaaaaacaaa ataggattga ttcctgaaga tagaaaaaat
    12061 ctaggattaa ttttagaaca agctatatac acaaactcat catcagtaca aattgataaa
    12121 ttcaagaaat tcttgtttat agatagaaat aaagaaattg agttttctaa aaaaggtgtt
    12181 aaggaattag gaataaaagt taatgatgta aagcaagaag taaaagagct tagtggtgga
    12241 aatcaacaaa aagtagtagt ttctaagtgg ttagagaaag atttagattt aataatatat
    12301 gatgagccga caaagggaat agatattgct gcaaaagaag atattttcaa tacaatcaaa
    12361 gaattttcaa gtaaaggaat aggagtaata tttatatctt ctgatttaga agaagtaata
    12421 agagtaagtg atagaatttt agtaactaga gaaggttcta ttgtagatga tttaaaaaat
    12481 gagaatatac aggttcaaga tataatgaac agtatattta atgtataaaa aatgaaaatg
    12541 gaggactgaa atgaagaaag cttttgagaa gttaaattta agagattatg gagtattatt
    12601 aggtttttta gctttatgta tagctataag tattgcaact ccatcatttt taggtaaaca
    12661 aaatatactt aatcttttac gacaatcttc tataattgga ataatttcag caggtatgac
    12721 ttttgttata atatctggaa actttgatat atcagttgga gcagtagcag ctcttgcagg
    12781 tgctataaca atgaaatttg cgactggtgg aactaatttg tttgcatcca tgctcttagg
    12841 tgtagcgatt tgtgcagtaa taggattaat caatggtata gtagttgcaa agataaatgt
    12901 accatcatta atagccacta tggctatggt cactattgta agaggattgc tgttgatgct
    12961 tacaggagga tatccaatta ctgaaaatat accaatactt gattacattg gaaatggata
    13021 tttgtttaat ataccaatac cagtagtaat tttctttata gtggtgttag cttcatttat
    13081 tgtacttaat aaaactaagt ttggtagata tgtttattct gtaggaggga atcaagaagc
    13141 aagtaaactt aatggtataa atgtagattc tcacaaagtg aaagtattta ttattaatgc
    13201 tgtacttgca ggaatagctg gaatcgtact aacaggaaga cttgggactg caactgcgat
    13261 tgcaggtgaa ggatatgata tggatgcaat tgcatctgta gtaataggag gtacatcagt
    13321 ggcaggaggt tctggttctg tacttaaaac tgttatagga gtattactta tgagtgttat
    13381 aaacaacagt tttaatcttc ttggaataga tgtatatttc cagtacatat ttaaaggatt
    13441 aataatttta gcagcagttg gatttgattc ttatagcaag aaaaaacttg cttcaagata
    13501 aaaaataagg gggatttttt tatgaaaaaa ttaatgaaac atttagcatt gttgctatca
    13561 cttgttatga tatttggttt agttggttgt tcaaatggtg gtgacaaaga aaaaaatgga
    13621 gaaaagaaaa tagcagtatt actaccaggt tcaacaggtt actttgtagc aactaaacaa
    13681 ggtatagatg caaaatctaa agaattaggt gtaagcgtgg aatatgcaga tgctcaatgg
    13741 gatgctagta aacaattatc acaagctgaa gattttatgg ctaaaggggt agacatgatt
    13801 gcattatgtg gagttgattc tgctgtaagt gagagaatag ttaaagctgc taatgattca
    13861 gacgttccta tagtagcatt tacaaattct ataggaagta acccaactgg agaatttaaa
    13921 ggtttagtta catatatagg acaaaatgag gaagaaacag gagcattaac tggaaaaatt
    13981 gctaaaaact tattaggaga aactggtggg aaagctgtat taatagaagg agtaccagga
    14041 actactccac aagtattaag aaaaaaaggt cttgaaaaag aattaaaaga tagtaatata
    14101 gaaatagttt ataatcaaac ttcaaattgg gagaaagagc aagctttaaa ggtaacagaa
    14161 gatttaattc aaaagaaaac tgattttaat gttataatat gtcaagatga taattctgct
    14221 acaggtgcag gtcaagcttt aaaagatgct ggtcttaagg ataaagttaa agtcataggt
    14281 ttgggtggaa gtaaagatgg attaaaagct ataactgatg ggttaataga tggtacaact
    14341 tatatgtctg ctgtagaaga aggtggatta gtcatagaaa aggcatctaa attccttaaa
    14401 ggtgaaaaaa tagaacctgt tacacaaatt aaacaagttg aagttaacaa agataatatt
    14461 tcagagttca agggtgaatg gtaaattgtc atagataata aataagattt ttagagttac
    14521 tttagaaact atataaagta actctttttt taatttaatt tagtgataaa ttattgaata
    14581 gtatttctaa acaaataaaa aattaatata aacaaagact taattatact aaattataag
    14641 taaaactaat ataaacttat ggaaaaaagg aaaacgtcta aagtattgga attactatag
    14701 tgtaggtgtg gtattaaaaa aacataaaaa aacataaaaa aattgttgaa aaaaatatag
    14761 aaattttgta taattaaaga tttgaaaagt gcggtataca aagccaattc ttttgtatat
    14821 aatatatata aattcagaaa aaggaaggga tatgtatggc caggcggtta tttttagaaa
    14881 ggattggact tatattttta ggttcagcaa tattagcatt tggagtttat aatttttatt
    14941 atttaaacaa cataacagaa ggtggagtgt taggtatttt actactgtta aaaaatttat
    15001 tcaatataca accagcaata gcaaatgttg taatagatgg tttgctactt ttagtaggat
    15061 ataaattttt cggtaaaaaa ttccttatat attcaatagt agcttctata actttttcag
    15121 tgttatatga cttatttgaa gctataggac ctctagtacc acaatcacaa aatatgcttt
    15181 tatcaactat tttggcaggt gtaactgttg gaactggtgt aggtatagta gtaaaagcag
    15241 gttgtgcatc aggaggagac gatgctttag cattagtaat ttctaagact acatctttga
    15301 acatagggca agtttattta gcaacagatg ttattgtatt gttgttatca ttattttatc
    15361 tatcagcgtt tgatatcttt tattctttga ttgctgttac aattagtggc aaggtaattg
    15421 attttattta ttatcatggt aaaagtttgg atatggatat aagtaatgat atagttccag
    15481 aatgttaata taaattaaat aactttagat tttattaggg gtggcagatt tgccaccttt
    15541 tttgtgtcta aatctttata gaaagtataa tcctatctag gtttttgcaa atctcaaata
    15601 tagaattatg ttgaaatgac tgggaatacg attgctagat gtgcatttta aaaacccaat
    15661 tttatattta tgaaatacta atcattaatg gatgtttctt aatcctatct ttatacctaa
    15721 aatgataaga taaatgaagt agcaatacaa aactaaaatt tgtaaaagag attaaattaa
    15781 atggaggagc taaaatgttg gatatagtta tgatagcatt gttattcata ggatttgttt
    15841 ccatgaagtt acttgctgat tggtgtggga aacaaactga aaaaaaataa tgtaatgtat
    15901 ggagggatta cctatgtggt ttctggcagt tgtaattata atgttaataa tatatttggt
    15961 atatgcgctt attaatcctg aaaaatttta aacagtataa ggctaataag taagttgaat
    16021 tggggggaac aatttttatg gtgcagatag taatagtgtt agcgattttt atgattttag
    16081 taatacctat gggcaagtat ttatatcata tagctactaa tcaaaaaaca tttggagata
    16141 gactatttga taaagtggac aattttatat ataaggtatg tagtatagat aagaaaaaag
    16201 aaatgaactg gaaacagtat gcattagctt tattatttac aaatgcagtt atggtattta
    16261 taggatatat catacttaga actcaaagta tgcatatttt taatccaagt ggaattaaaa
    16321 gtatggaaca aggactatca tttaatacaa taataagttt tatgacaaat acaaatcttc
    16381 aacattattc aggtgaatca ggactttcat actttagtca gatgacagtg ataatatata
    16441 tgatgtttac atcagctgca acaggatatg cggctgctat ggcatttgta agaggtctag
    16501 tcggtaaaaa gaaaacattg ggaaattttt atgtagattt aattaggata acaacaagag
    16561 tacttttacc aggagcttta attattgggc ttatattagt tacacaagga gtaccacaaa
    16621 cttttgctgg gacagaaaca gtgactacta tagaaggaaa gcttcaagat atagctagag
    16681 gtcctgtggc agctcttgaa tcaataaaac atttaggaac aaatggtggt ggattctttg
    16741 gttcaaattc atcacaccca tttgaaaatc caacaataat atcaaatata gtagaaatat
    16801 tatctatgat gatattacca ggagcttgtg ttgttgcatt tggtcacatg ataaagaata
    16861 aaaaacaagg ttgggttgtg tttggcgcaa tgtcaatcat atttttaata ggtctagtag
    16921 tgtgctttaa agcagaaagt gcaggaaatc ctattttatc acaattaggg cttaatcaaa
    16981 gtatgggaag tatggaaggt aaggaagtta gatttggtat agcacaatca tcattattca
    17041 caacagtgac tacttccttt acgacaggta cagttaataa tatgcatgat acactgactc
    17101 ctttaggagg attagtacca cttttaaata tgatgctaaa tgtagtattt ggtggtaaag
    17161 gcgtaggtct gatgaatatg ttgatgtacg ctataatagc agtattctta tgtggattaa
    17221 tggtaggaag aacaccagag tttttaacta agaaaatcga aggaaaagaa atgaagctta
    17281 ttgctttact tattatatta catccacttt taatactaat gttctctggt ctttcagtag
    17341 caataccagc aggacttgaa ggaatttcaa atcctggatt ccatggatta tcacaggtat
    17401 tgtatgagtt tgcatcttca gcagccaata atggttcagg atttgaaggt ctaggagata
    17461 acactatgtt ttggaatata acaacaggta tagttatgtt ctttggaaga tatgtgtcta
    17521 ttatagtact actagcaata tcaagtttgt tagcatctaa aaaagctgta aatgaaagta
    17581 taggaacact tagaacggat aattttacat ttacaatagt tttagtactt gttgttttaa
    17641 ttgttggtgc tttaacattt ttcccagcct tagcgcttgg acctatttcg gagcatctag
    17701 tgttgtggca ctaaaaaatt gggagggagt aaagttttat ggaaactaaa aaaacaaagt
    17761 ttattacaaa agatattttc aaaacttcca ttatagaagc ttttaaaaaa ttaaatccta
    17821 aatatatgat gaaaaatcca gttatgtttg ttgtagaaat tggatttata gtaacattga
    17881 tacttacaat agtacctagt ttgtttggtg atgaaggcaa taacttaaga gcttataatg
    17941 ggattgtaac aataatctta tttataacag tcctttttgc aaattttgct gaagctgtag
    18001 ctgaaggaag aggtaaagct caagcagata cacttaagaa aactagaaaa gatacagtag
    18061 caagggttat agatacgtta ggaaatgaaa aaatgataag tgcaagtgag ctaaaacttg
    18121 gtgatattgt tttagtaaat gctggagaag ttatcccaaa tgatggagaa gttatagagg
    18181 gtatagcttc tgtggatgag tcagcaataa ctggagaatc tgcacctgtc atgagagaat
    18241 caggtggaga tttcgcatct gttacaggtg gtactacagt tgtaagtgac tggttaaaga
    18301 taagaataac aacaaaacca ggagaatcgt tcttagataa aatgatagct cttgtagaag
    18361 gtgcttcaag acaaaaaacg ccaaatgaga ttgctttaaa tactttgtta gtagggttaa
    18421 cacttatatt tatggttgtt ttaatatctc tttatccaat ggcaaactat gcaggtgtaa
    18481 agattccaat ttctactatg atagctctct tggtatgttt aataccaact actataggtg
    18541 gattgttgtc agctattggt attgcaggta tggatagagt aactagattt aatgtaattg
    18601 caatgtcagg aaaagcagta gaagcttgtg gagacgttga tactatgatt ttggataaga
    18661 caggtacgat tacttatggg aatagacttg catcagaatt tatagcagtt ggaaatgcag
    18721 attcaaaaga tttaataaat tattctgtta tgtgttcatt aaaagatgat acaccagaag
    18781 gaaaatctat agttgattta ggtatgaaat taggttcaac aggtaagaca aaagaggcag
    18841 aggaagctga gtttgtagaa ttttctgcac aaactagaat gagtggtatt aacttagctg
    18901 atggtagagc tataagaaaa ggtgcttatg atagcataat aaaaagagta aaagaatctg
    18961 gtggtacagt gccagaagat ttagaagaaa atgtaaatcg agtagctaaa ctaggaggga
    19021 cacctttagt agtgtgtgta gataatgaga tatatggaat tatctactta aaagatacag
    19081 taaaatcagg gcttgtagaa agatttgcta gacttcgaga gataggaata aagacaataa
    19141 tgtgtacagg ggataatccc ttgacagcag ctacaattgc aagagaagct ggagtagatg
    19201 gatttattgc agaatgtaaa ccagaagata aaatagatgc aataaaaaaa gaacaattag
    19261 agggtaaaat agttgcaatg acaggtgatg gaacaaatga tgctcctgca cttgctcagg
    19321 cagatgtagg tcttgcaatg aatagtggta caacatctgc aaaagaagca gcaaatatgg
    19381 tggatttaga ttcagaccca actaagatat tggaagttgt tgaaatagga aaacagcttt
    19441 taataactcg tggggctttg acaacattta gtatagcaaa tgatgttgca aaatattttg
    19501 cgattatacc agctatattt actcttgcaa tacctcaaat ggagatatta aatgtaatga
    19561 aactttctac gtcatttagt gcgatattat cagctttaat atttaatgct ataattatcc
    19621 catgtttaat acctcttgca atgaagggag taaaatatag acctatgaaa tcacaagcta
    19681 tgttaatgag aaatatgctt atatttggtc ttggtggagt tatagtacca tttttaggaa
    19741 taaaattaat tgatttattg attacacctt tagtaatggt tcttaattta ggttaataaa
    19801 tggaggatat aaaaatgaaa acattaaagc cagcattact tgtaagtatt gtattgttag
    19861 ttgtttgtgg acttgtatat ccacttgttt tgacaggtgt aagccaagtt gcatttaaag
    19921 ataaagctaa tggaagtatg atagaagtaa atggtgtaaa agtaggttca gaacttattg
    19981 gtcaaagctt tacagatgcg agattcttta agggaagagt atcatcagta aactataata
    20041 cttatacaaa agaagattta gtaccagata aagatggaaa cacatcttat ggaggtgtat
    20101 catcagggtc ttttaattat ggagctacaa atccagaact tcatgataga gtacaaaaag
    20161 atatagaaca gtttttaaag gataatccaa ctgtcaaaag agaggatata ccaacagatt
    20221 tacttacagc atcaggttca ggacttgacc ctaatataag tccagcctct gcaaaaattc
    20281 agataccagc tatagcaaaa gcgtctggaa tatcagaaag taaattacaa aaaattgtag
    20341 aagataatac ttccaaaaaa ctttttggag ttttaggtga agatagagtt aatgtattaa
    20401 aggttaatgt agaggtcgca aaaatattag gtcttatata gtattttgtg gaattttagc
    20461 aacaaaatat tactcaagat ttaatttaaa ggttaaattt taaagagtga aaatcgataa
    20521 tgattttcac ttttttattt gaatattact aatatttgga ataaagtgat tgtaagtata
    20581 aaatccaaaa aaaggttgca ttatcaaaat gataatacta taatatattt atcgttatca
    20641 atttgataat agatttggac atataggagg aaatagtatg ttatataata acgcattaga
    20701 gttgatagga aacacacctg tagtaaggct gaataattta ggctgtacta atgggtgttc
    20761 taacatatat gtaaaattag aaaaagtaaa tccagcagga agtataaaag atagagctgt
    20821 ttatgaaatg ttagagggtt tagagcaaag aggagaatta aaaaagggag atgtactagt
    20881 tgaagcaact agtggaaata ctggtattgc attatctatg ataggaaaat taaaaggata
    20941 tgaagtagta atcgtaatgc ccgaaacaat gagcgttgag agaagagatt taatgaaggc
    21001 ttatggagca aatttggtgc ttacagatgg taaagctgga atggcaggtt ccatagaaaa
    21061 agcaaatgaa cttttaaaag aaaatccaaa ctataaaagc ttaaaacaat ttgaaaataa
    21121 agataatcca aatgcacatt ataaaacaac agcagttgaa attatgaatg atgtcaaaga
    21181 tttagatata ttcgtatgtg gagttggaac aggtggtact ttaataggaa ctgcaagata
    21241 tttaaaagaa caaaatccaa atataagagt aattgcactt gaaccaaaaa aatctccagc
    21301 tatatcagaa aacaagactg gacctcataa aatacaagga ataggtgcta actttgttcc
    21361 tgaaaattat gattctagtg tagttgatga agtaatatta gtagatgatg aagatgcatt
    21421 tgatacagtt aaattacttg cagcaaaaga gggaatttta gtagggatat cttctggagc
    21481 aaatatattt ggtgctatag agatggcaaa aaagtatcca gataaaaaga tagttacagt
    21541 agctccagat ggagttgata agtatatgtc tatgggaata ttttaataat tttatttgag
    21601 ggagtgaata ctttgtttaa gaaaataaat aaagatattg aatatataat gaagaatgat
    21661 ccagcagcta ggtctaagat agaagttttt ttactttatc catcagttca tgcaatgata
    21721 atgcatagga tggctcatgc tctttataag aagaagaagc tttttacagc aagattaata
    21781 tcccagatat ccagatttat gactggcata gaaatacatc ctggtgctaa aatgggggaa
    21841 ggtatattga tagaccatgg aatgggtgtt gtaattggag agacagctga agtaggtaat
    21901 agggtgacaa tatatcaagg agctacttta ggagctacag gaaaagatac tggaaagaga
    21961 catccaactg ttggtgatga tgtattaatt ggtgctggaa caaaaatact aggaccttta
    22021 aatataggtt caaattcaaa aattggtgca aattctgttg tcgttaagga tgtacctaat
    22081 ggtgcaacag ttgttggtat accagcaaaa atagtaaaaa taagaaacct agaacctgta
    22141 aaaaagaata aaaaagaagt gtcctatgag tatgatgaat tagataatgt atattatata
    22201 taggatttat ataactataa aataaacttt ataaaaatta ttttaaaagg tatttttttc
    22261 aaatttgagg acataataaa aaagaactga atgttacagt tttggttcat caatctttga
    22321 taaacaagat tttaaatttg tagcgtctag gaggatttta ttatgaaagc aaatgttaat
    22381 caagatactt gtataggttg tggattatgt ccatcaatat gtccagaagt atttgatatg
    22441 aaggatgatg ggaaatcaca tgttatagtt gatgaagtac caagtgatgc tgaagaatca
    22501 gcagcagaag ctagagaaag ctgtccagtt gatgcaatag atgtgcatta ataaaataaa
    22561 tgcctataaa aattgaattt tcaattttta taggcatttg tcttttattc agttagtatt
    22621 tcaacacctg tttctgtaac taaaaccata cattcccatt gagcagataa tttaccatct
    22681 tttgtataaa tagtccaatt attttcttca tcaacaaaaa tttcgtgagt tccttcattt
    22741 accataggtt ctattgtgaa tatcatccca ggaaccaata gcatctcagt ccctttttta
    22801 gatacataac ttacaaaagg gtcttcatga aagcttaatc caactccatg accacctatt
    22861 tctctaacaa cagagtatcc atttttcttt gcataagagt tgataacatc acctatatct
    22921 cctaagaatc cccatggttt ggcaacttca agaccttttt ggacacattc ctttgtaact
    22981 tgaactaatt tttttgcatt ttcgttaaca ttacctatca taaacattct tgaagcatct
    23041 gaatagtatc cgttaaaaat agtagaaaca tctacattta taatatctcc atcttttaag
    23101 ataatgtctt tacttggaat accatgacaa acttcattat ttactgatgt acatacactt
    23161 ttaggaaatc cattatagtc tagtggagca gggatagctc cattttgtag tgtaaagtca
    23221 tgaaccaact tatttatatc ttctgtagac attccaattt cgattttatc tgaaatataa
    23281 tccaaaattt ttgtgtttat tacagcactt ttttttattt cctcaatttg actttcattt
    23341 tttataatat catgagttgg tacaatatga cctaacttag caaatgaatc tattttatta
    23401 tccatatcca tatgacattt tttatatttt ttagtacttc cacaccaaca gctatcattt
    23461 ctatttaact tcataacttt tcatccttct tttattattt atttaaatta tgggttacat
    23521 aaactatttt aaaacataac cattataaat attataacat aaatactaag aaatccttta
    23581 tagatagtga tttattcaaa gtataaatta ttatatggtt attgttgatg aaaagaggat
    23641 agttaaatgt attattggat attagcgcac ttaatagttt agttcaagtt tttcaagaaa
    23701 atcatatgtt agaggacaat tttcctttaa agtatttttt gaatcttcat caagataata
    23761 atgtacaaat gattctgcaa aaaattcttc tataaacttg cttatatatg catgtgcaac
    23821 ttgcttaggg tcataaagct tatatctttc ctgagcaaat acttccataa attcttcaga
    23881 atttgatttt ttatttaaaa catttttatc tatagcatgt gcagtttcat gtaattctaa
    23941 attttttgat gaatgtacat ttgcatagct agggttacta taacctattt tagccactat
    24001 agaattattt ctatatatac caggtacatc tttccaagtc ttgcctgatc ttacccaaca
    24061 tggaggtaat tgatatttta aatcactaaa ttctggttca tcagttagat ttgaatttat
    24121 taattttatt tttatatcct tttctttaag tgtctgtaaa tatttaatat ttatttttgc
    24181 aagtctgttt ttcatatttt caacttcatc tttatcataa ttcaagtcat caggaagtat
    24241 aagcatttgt tcaattatat ctatactact gtcttttata ttttcagatt cttcatcaaa
    24301 tttatattga agagtattat tttcatagat agttatattt gtattatttt ctatagaata
    24361 agagtgaata tcctctaaat ctaaaatatt catttgttgt aaaatatgtt ttgtttcgcc
    24421 actccacact actaaagaaa gtgaattaaa attaattagt ggattttctt ttgcttttat
    24481 atcattgctt atatctacat caaatacata ttcaattgag tcttctatgt taagactatc
    24541 tgtattcttt ttaatatata aatacgcttg ttttaagtct atataaatct catctaaaga
    24601 ttcgtcataa aattctgata aattattagt aaagagttct tcacttaaaa gttcattttt
    24661 atcagttata ctttcaatta aaatattaat ttcttttatc atattgtaaa tacttatttt
    24721 tctatttgct ttttcactag atacattgca tggcgcaaga gcaatgcaat aaaaatcttt
    24781 ttctttttta taaaaggctt taagtgattt tgataaaagt gtaaagtcta cacctatctg
    24841 taagttgacc ttaagtgagt cattttgtaa taattcagta ttatttcttt tagctgtatt
    24901 taaattgttt tccataatca ttgccccctt tttattaggg tatcataatt tttttatatg
    24961 tgtcaatttg tttttttgaa aattataatt tttttgaaaa ttttattttt tataatttgt
    25021 gaatttttta ttatagaaat aaatatgtta ttattatgtc aaaatttagc attaga
//
LOCUS       DACULQ010000040        25061 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.040, whole genome shotgun sequence.
ACCESSION   DACULQ010000040 DACULQ010000000
VERSION     DACULQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.040"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..896)
                     /locus_tag="JA154_06570"
     CDS             complement(<1..896)
                     /locus_tag="JA154_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAT7995528.1"
                     /translation="MKLKHIAIIGSLFPILFSIVLFFGVLMSADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAKEYGISEYVNVLLAIIQVESGGTAKDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGINDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQMQYDATQHIPLSQA
                     KAGELVFFHSTYNAGSYVTHVG"
     gene            complement(893..3085)
                     /locus_tag="JA154_06575"
     CDS             complement(893..3085)
                     /locus_tag="JA154_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAT7995529.1"
                     /translation="MKERIQSAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPENEDGKTREEVVKTEIESNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYMAHRARRMERRLAGAVTAGGVAGAVAGSTVSNARTTRNNSAKDNRGNTSSSMGQR
                     AGSKVGAVLDTKNKVKDKANAMKETVKDIPTQTAYAVHSAKEKAKTSVSDFKRGMVQE
                     QQSRQSGRLEKQEQHKKNIADKRMELQKAQEARQARKTDGSATTGATRPHERPVTASA
                     IPKTNVEKTQEVKRPATTSTSKAGEPVKTNSVKERPLSSGTSDGKIPQSAQTVHSKNV
                     EKVVSHETHQNYKAERTTKAQTVEQAKRTTEHTEKNRNLVTKKGQKKK"
     gene            complement(3085..5535)
                     /locus_tag="JA154_06580"
     CDS             complement(3085..5535)
                     /locus_tag="JA154_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAT7995530.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNLKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEHQLPKKKYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSETRGLLHVIDELRCEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGEILSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPIQRNEVE"
     gene            complement(5522..5911)
                     /locus_tag="JA154_06585"
     CDS             complement(5522..5911)
                     /locus_tag="JA154_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995531.1"
                     /translation="MKKIRSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPAFLTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLQKEIFNETITAVRSVSYVPD"
     gene            complement(6029..6532)
                     /locus_tag="JA154_06590"
     CDS             complement(6029..6532)
                     /locus_tag="JA154_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAT7995532.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIEIGEYVSIDELNEMYEMIEELPDYIVDCLDEFIGHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            complement(6535..7038)
                     /locus_tag="JA154_06595"
     CDS             complement(6535..7038)
                     /locus_tag="JA154_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAT7995533.1"
                     /translation="MQETRVLVETKRTTGEETTSYWFDLPIEVAEFEEKLGVSTESQD
                     YRIIEKLLPYADEVHEHTSVYQLNELDYMYRQLSSDMQEEYTMLHTVYENLEALYICR
                     NVITLYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFETYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(6957..7253)
                     /locus_tag="JA154_06600"
     CDS             complement(6957..7253)
                     /locus_tag="JA154_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995534.1"
                     /translation="MNDIFRDMQAKVGCENISDLPSYKRKVWHEMKQLNLADYEEKQL
                     EDFSKYVFGMSCQTLKDVMKQQKGREEQCRKQGYWWKRKEQLAKKQHHTGSTCR"
     gene            complement(7350..7952)
                     /locus_tag="JA154_06605"
     CDS             complement(7350..7952)
                     /locus_tag="JA154_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7995535.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            complement(8037..8198)
                     /locus_tag="JA154_06610"
     CDS             complement(8037..8198)
                     /locus_tag="JA154_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995536.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALVAVGLVFNAGGVKDVLL
                     ELFNKIIGA"
     gene            complement(8365..10275)
                     /locus_tag="JA154_06615"
     CDS             complement(8365..10275)
                     /locus_tag="JA154_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAT7995537.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNHILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11017..11151)
                     /locus_tag="JA154_06620"
     CDS             complement(11017..11151)
                     /locus_tag="JA154_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995538.1"
                     /translation="MWELLKDLLLVSFGMGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            complement(11159..12280)
                     /locus_tag="JA154_06625"
     CDS             complement(11159..12280)
                     /locus_tag="JA154_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAT7995539.1"
                     /translation="MANISREHLSRIEAGKVTLTEDMKHTLLEAVEKFNPNNPMFLLF
                     DYVRIRFPTMDIKHIIKDILKLNIGYMLHEDYGHYKYTEHYHIGDVFVYVSQDEEKGV
                     LLELKGKGCRQFESYLLAQERSWYDFFTDALVEGGVMKRLDLAINDRTGLLDIPELIR
                     KCENEECISKFRSFKSYGSGELVKHNETDKYGMGHTLYIGSFSSEVYFCCYEKNYEQY
                     AKLGIPIEEVPIKNRFEIRLKNERAYYAVVELLTHYDAELTAFSIINQYIRFADKEPD
                     KRKSDWKTNVRWAWFIGEGRPPVKLTTQPEPYTLERTLNWLQRQVAPTLKMLKQIDKD
                     NQTEYLETIEKQAKLTERHYQIIKQQTATVEEMVANVKE"
     gene            complement(12560..13954)
                     /locus_tag="JA154_06630"
     CDS             complement(12560..13954)
                     /locus_tag="JA154_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT7995540.1"
                     /translation="MKMILNKGHRIRASDKNLVYHFSIGTLLFVFIAVILLLNMKQLM
                     RTDWEHFSLLDNGLTLSTYNFITLLIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIIWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTMLYDMIANRITIDEVKAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFSSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            complement(14071..14556)
                     /locus_tag="JA154_06635"
     CDS             complement(14071..14556)
                     /locus_tag="JA154_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995541.1"
                     /translation="MAKEKGSFKEFLSAIAPEHQAFVEKLNTKLIEQGCDLVIKEAKS
                     GYTASYQLEKKTVMNWVFRKTGVFARIYGDNAGKYEDIIASLPADMQKKMTTSRDCKR
                     LIDPNACSDTCVKGFVYALNGDTHKKCRNDGMLFVLTNETAEHIAGLVCAEVNVRKSA
                     L"
     gene            complement(14654..15043)
                     /locus_tag="JA154_06640"
     CDS             complement(14654..15043)
                     /locus_tag="JA154_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995542.1"
                     /translation="MIMRLANGIVLDKEKTFGVLKFSALRHEVRAENDDGSTSEEITR
                     RTYDLKCSVQERMIQVSIPADIPVKDYPYNAEVELVNPVADTVANASYRGVNVDWYIK
                     ADDIILKNKGGQVGNPQNHAPQGQPKK"
     gene            complement(15052..15378)
                     /locus_tag="JA154_06645"
     CDS             complement(15052..15378)
                     /locus_tag="JA154_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995543.1"
                     /translation="MELKYVVPNMAQTFGNLEFAGENKIEQQRINGRFTPVSRSYNLY
                     SDVQRADDIIVTIPAKAGEKHFKPEQRVKLINPKITADGKAVKGRGFTNYILTADDML
                     PAEESK"
     gene            complement(15385..15601)
                     /locus_tag="JA154_06650"
                     /pseudo
     CDS             complement(15385..15601)
                     /locus_tag="JA154_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843347.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15614..18658)
                     /locus_tag="JA154_06655"
     CDS             complement(15614..18658)
                     /locus_tag="JA154_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HAT7995544.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPTTLVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTDNHNGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITNGNG
                     NYSIAGATYGVFSDKDCTKQIATLTTDDSGNTEVAEVKATTVYIKELSAPLGYKVDTT
                     VYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKANPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTTAETDSNGTTHYITKLADAYKVSGDSFYMQDGKAFLPL
                     GTLTVEETKSPTGYLLDGAYMQANGSEEQIKALYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETKDTKPQGSATLKDTAFDIISLNDNAVLVEGKIYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPNGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSSKWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGMELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEITIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAEIIIDGKRVESDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPMKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTKYKLVGWQMLKEENAELLINGKRVESDYTFIADSETMKVEVTFTFDATSL
                     DGKQLVTFEELYDLSNPNEPKKVTEHKDIEDEGQTITFKEKPEVPEKPETPTTPEKPS
                     RPTDSPKTGDNTNVMAFLVMLLTSAGGLAGTYLYKRRKMKKS"
     gene            19186..20193
                     /locus_tag="JA154_06660"
     CDS             19186..20193
                     /locus_tag="JA154_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit A"
                     /protein_id="HAT7995545.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKDVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            20194..20985
                     /locus_tag="JA154_06665"
     CDS             20194..20985
                     /locus_tag="JA154_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HAT7995546.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            20998..21969
                     /locus_tag="JA154_06670"
     CDS             20998..21969
                     /locus_tag="JA154_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HAT7995547.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            22026..22802
                     /locus_tag="JA154_06675"
     CDS             22026..22802
                     /locus_tag="JA154_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088744.1"
                     /note="member of the FNT family of formate and nitrite
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAT7995548.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMILLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            23070..>25061
                     /locus_tag="JA154_06680"
     CDS             23070..>25061
                     /locus_tag="JA154_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAT7995549.1"
                     /translation="MMTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAML
                     TLLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDL
                     KRAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKE
                     LISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDD
                     FYISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVA
                     TKEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKK
                     VKSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHL
                     RDGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLL
                     AGYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFIL
                     PYDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINA
                     IYKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDE
                     GRSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNI"
BASE COUNT         7331 a         4816 c         4635 g         8279 t
ORIGIN      
        1 attcccacat gggtaacata cgaaccagcg ttataggttg aatggaaaaa gaccaactcc
       61 ccagcttttg cctgcgataa agggatatgc tgggttgcgt catattgcat ttgtgccgtt
      121 ctcggtaaag agataccagc ttttccataa caccattgcg tcagtcccga acagtcaaag
      181 gaagtgttgg gattgctacc gccatacaca tatttccagc cttgatattt caacgcttca
      241 ttcattacct tttgtgccag ttctcccgat acctgcggaa cagttaaata ctgattgact
      301 aattccacat aaaacatatt tccatagcca taccgccagc ccccattttt cgcaacggct
      361 atcgggttgg tgtaggttac ttttttaccg ccagatttct cacgggcaaa gctctctgca
      421 aggttaaaag tatgtttctt tccttttcct gccacatatc ccacatagcc accgccatag
      481 ttataggact gtatcgccac attcaaatca tttattcctt gatttttgca ggaagaaagc
      541 agggacgcaa aatatttaca cccctgcttg attgagcttt ctgtatctaa cgagttgggc
      601 ggtaaaccaa gactttctga actctgcatg acatcttttg ccgtaccgcc actttctact
      661 tgaatgatag ccagtaacac attcacatac tcagaaatac cgtattcttt agcgtacttt
      721 tccaccatag gctgatgttt caagacttct gcggataaat tcatacccgt aatgcccgaa
      781 gaaaagttgc tgttctcgtc gtcgctgtcc gcactcatca gtaccccaaa gaaaaggact
      841 atggaaaaga ggataggaaa cagactgcca atgatagcga tatgtttcaa tttcattttt
      901 tcttctgtcc tttcttcgtt acaaggttac ggttcttttc agtatgttct gttgtacgct
      961 ttgcttgttc aactgtttgt gcctttgtgg tacgttctgc tttgtaattc tggtgtgttt
     1021 catgcgatac cactttttct acatttttac tatgtactgt ctgtgccgat tgtggaattt
     1081 ttccgtcaga tgtacccgaa gataagggac gttctttcac actatttgtt ttgactggct
     1141 cgcctgcttt ggaagttgaa gttgtagcag gacgtttgac ttcctgtgtt ttctcaacat
     1201 ttgtttttgg aatggctgaa gctgtgactg gtcgctcatg gggacgggta gctcccgttg
     1261 tcgctgatcc gtccgtcttt cgtgcctgcc ttgcttcttg tgctttttga agttccatac
     1321 gcttgtcagc gatattcttc ttatgctgtt cctgcttttc caagcgtccc gactgtctgg
     1381 attgttgttc ctgcaccatg ccacgcttga agtccgatac acttgttttt gctttttcct
     1441 ttgcggaatg gacggcataa gcggtctgtg tcggtatatc cttgaccgtt tctttcatag
     1501 cgtttgcctt gtctttgacc ttattcttcg tatctaagac agcacccacc tttgaaccag
     1561 cacgttgtcc catgctggaa gatgtattcc ctcggttgtc ttttgctgaa ttattccttg
     1621 ttgtccttgc attagaaacc gtactgccag ccaccgcacc agcaacaccg ccagcggtaa
     1681 cagcaccagc aagccgtctt tccatacgcc tagccctatg tgccatataa agatacggtc
     1741 tgcggaaaat tcttcgtccc atgctttgac tgtcgttagc gttcaaagag aacatactca
     1801 ttaaatcgcc taacttcatg tagataccag cgaaacacac tatctgcaag aacgccacca
     1861 taaagaacgg gtagtctgtg gaaatgttat aaaacatact ggaaatgcta aatgccaccg
     1921 ttacaatgag cgtaattcct gcccttgtca ttatggtatt aaataccctc acgattgcct
     1981 gctttgccat gctttcatag ctcggtatca tggatagtaa aaagctgata ggtaaaaaca
     2041 tggcgaagat aataaaaagt atctggctga acaacatcat acccgtaagc aagaatacaa
     2101 atatcgtgat acctaagttg aagaacaaga ggaagaacac catacctaaa cggtttacga
     2161 cttgcggtat cgtcagattg ttgttgtcgt tactttcgat ttctgttttc acgacttcct
     2221 ctctggtttt gccgtcctcg ttctctgggc ttgccgatac aagagcttct acacggtctg
     2281 tcccgatttc ctctgcgttg ctgttcccaa actgtaagag tagccacggc tgttccactt
     2341 gaatagagaa taggctgtcc cttatcaagt ccacgctgtc cttgccctcg ctgtcagagt
     2401 ttgggagcat gatttttgtt cccaagtcca aagaagcggt actgatgtcg cttgaaaatt
     2461 catttatctt tttgatgtag tcgggagcat aggcaataaa tgaagccgac aaaataaaca
     2521 ccacaacaaa gttgataacg gcgtgaagtg ccttgctggt ttctctttta atcagtcccg
     2581 tataggcaac atatagtccc accactaaga taatgagaag caggaaaccg acatagaagc
     2641 cagaggttga aaaaccgctt gaggtaacgc ctgcaagggt ctgtatgctc tgcccgatac
     2701 tgtctgccat atcattgatg aagtccagct tgtaggcttc ctgcactaca tagcccgtgg
     2761 cattacttaa atataaactg atagtccaga caaaattagt gatacagtac aaaccatatt
     2821 gtactgattt tccaatcccg tccagccagt tccacggcaa ccaagcccag ctattatcca
     2881 catagaagtc aagctgatag ttggaaagcg gatatttgga atatagattt tctgcgttta
     2941 tcgtatcgtc cacaagcccc gtcgcatgag ccaccgtccc cagaagtgaa agcaggataa
     3001 gggagagtgc caccacgaac agagccattt tgagaaagtg gaaaatcttc ttttttgtga
     3061 acgcactctg tatcctttct ttcatcactc cacctcattt ctctgtatag gcggtctggt
     3121 atcaaaggcg tgtagcagtt cttcaaagac ggggtgtatc tgtaccacgc cgacacgccc
     3181 gtataagtcc tgcaacaagc attgtccgtt ctccaaatca cgaagtcgct tctgattgtt
     3241 ttcgtcctct ttgtcgatac cgaaaaattc taaagtctgt tttatctcgt tgatgtcagt
     3301 agaacggaaa gcgaatttta agccgatatt gtttttcaga ctttcctttg acacgtcccc
     3361 agaagattgc gtaacgaaat acacacctgc ctgcatagct cgtccagcac gcaccagctt
     3421 gttagataaa atttcgcctt gtgccacatt taagaacgcc cacgcttcat caaggtctac
     3481 gattttgaag atagagcggt cgctgtggat aaaatctaag gcaaaggtac taatcacaat
     3541 cagcatggaa accgatagta attcaatggt cgtgtattcc tcaaaagtcg tgtccttatc
     3601 tggcagtaca aggtcggcta cttgaatgat attgagctga ttatctaaac tgatagcatt
     3661 ttccaccgtt ccgtctgaaa acagaagatg tgcaaaatca tagtctgtaa agctgtcgat
     3721 atggtctgcg atattgcgtg aaattgcggt atcttcacag cgtaactcgt ctatcacatg
     3781 aagcaagccc cttgtttcac tctgggtaac agaacgcacc gccttacgaa gtacggggaa
     3841 tttttcgccg tccctagagg aaataccagt taaaaaggtt agtatgtcga ttgccagact
     3901 ttcagcgtct ttcacattct tcataatcac aaacgggtca agaagccctg cgttgtcctt
     3961 gtcgcttgtt aggtttacga tattgatttc atgggcgatt tctggaagtg tttctttcca
     4021 gtttccacgc tctgattttg ggtctaagat aaccgcttga ccgccaaaca aaacagaata
     4081 gtacacaagc aggttattac agaatgactt cccaccgcca agcgaaccaa caaaagcaga
     4141 agcaagggca tttgtaactg taccttttac gccttgactg gcaagggacg gttgcagata
     4201 tacatttctt cccgtatcaa cggaatagcc catatagata cccgtgtttt cccctaactg
     4261 ctgtgtcgca ccaaaaccaa gcccagctaa gaagtctgat tttacatact gcacataatc
     4321 attgatatat cgcttgctgg ctggtagaaa ttcagaatga agccccagca tatccccagc
     4381 aggacgtact aacttcacat tcaaatcgtc ataaaaatct ttgacttcat cacaacggcg
     4441 tttcagttcg tccaaatcat cagcagacac acggataaca taagagagct tatacatact
     4501 ttcttttgtc tggtctaaat cggtttccag ctcgtccacg ctgtctaaag cgtctactac
     4561 atttgagctg gtttcacttc cagcttgata agcgtgatta tcaaggtctt tcagttcctt
     4621 tttcttgttg cggacggtcg ttaatgcttt tcggttttcc acgatttcta cattcatgct
     4681 cgtatcaacg ggaaaggtaa attgctgttg ctggaaatag aagatttcac tagacggaaa
     4741 atcaagctcg cctacaatcg cattgacggt aaaataggac acataacttt ccttgtcctc
     4801 atgttccaat cgtaaatatc gctggctttc ctcaatcaca caccttgtcg ggcggataag
     4861 gtcgtaatac ttgataagtg tttctttctt atactttttc tttggtagct ggtgctcata
     4921 atcttcatag gcgataccgt ccctgccgta aagatgttcc ataagatacc caaagtcatt
     4981 gatttccaga cggcgaacct taaagcgacg ggagatttta ttttccagta acttttccat
     5041 tttcatgtaa cggttgattt catcattcgg catggaaaca aagtcgttca tcagcgtgtg
     5101 gttcacttca tggagaaact ctttgaacgt cagccacgcc gattttttaa ggttcttcag
     5161 attgagctgt tcttccgtaa ccatgagttt aaagccaaga aaaaagcggt agtccacttg
     5221 attgtcccca atcattgata ctaacgcttc ggtctgttcg tctatcttct ggtaagcgac
     5281 ttcctttaat ttccccgtaa ccaatctttt tgactgctcc tgcatacttc ttatagagct
     5341 ttccgtggca atctgcaaag catgaatttt accctcacgg gactgtgcga ttagctgtcg
     5401 gaaactgtca tgcacgataa atttctgttc tgcggataag aaagaatagt tatacggtat
     5461 cagctcatag taggcgaaca cctcattgtc cttgttccag acaaggttgt tgtcaatata
     5521 tttaatcggg aacataactc acactcctta ctgccgtgat tgtttcgttg aatatttctt
     5581 tttgcagttt tacggctttt cctgcataag tgattttcgg tcgcaacgca taggttatct
     5641 gtgatttcaa aaagctgtat ggcttctttc cgtcaaaggt tttctgcgac ataaaccaag
     5701 tgaggaaagc aggaataccg aagtatttta aaaatgctcc ctcaatcatg gaaagtgggg
     5761 gcatatcccc aaacagaatg atgataaatt ctgtaatcac aaaccatgta atctgtgtaa
     5821 aggtaacggg aaagggcaag ttaaagtcat tgattgcata caagactttt tctacattcc
     5881 agatacccgt gtagcttcta atctttttca agttctttca actcctctca taagtttcta
     5941 aatggaaaag gacagccctg tttcagactg tcccttaaag agaaatacgc cgtcagtagc
     6001 aacaatgctt gtcgctgatc ttccagcgtt aatatggtat ctcgcacatt cctcggcttg
     6061 ttgcaatgaa gcaaccgccc atatccaaat ctcgcccgta tgcttcatag tcaatatagt
     6121 tctgcaagct agggggaata tcgccaagtg cctgcaattc atctatgtag taataggcaa
     6181 catctgtcat ggtttcacag tcgggataat agtaaatatc gtccttgtgt tccacgactt
     6241 cctccagcgt cccataatga ccaataaatt cgtccaaaca atccacgata tagtcgggaa
     6301 gttcctctat catttcatac atttcattga gttcatcaat ggaaacatac tccccaatct
     6361 caatcgggaa gttatcggta tcatggattg cgtattcttc atactgttca ttcaagccga
     6421 ttttttcttt cacatcttcc tcgtcaatag ggaaagtaaa ccaagcaccg actaaatagc
     6481 cctcattata cttgccaaga ttagcgatat aaactgccat atcatcaatc atacttactc
     6541 tggcaattca aagataccgt gttccgttac taaaaatttc ccgtgttcct gcaagtgtga
     6601 agcataggtt tcaaagtcaa aatagtattc ttgacagtcc tcggataaat gtttgaatgt
     6661 agggtcattc atcagctttt gccttgcaac atcaatcatg cttttacagt cgggataaag
     6721 cgtaattaca ttcctgcaaa tataaagtgc ttctaaattt tcatacaccg tatgaagcat
     6781 tgtatattct tcctgcatat cgcttgaaag ctggcgatac atataatcta attcattgag
     6841 ctggtacacg cttgtatgct catggacttc atcagcataa ggcaacaatt tttcaataat
     6901 gcggtaatct tgactttctg tactgacacc taacttttct tcaaactcgg caacctctat
     6961 cggcaggtcg aaccagtatg atgttgtttc ttcgccagtt gttcttttcg tttccaccag
     7021 tacccttgtt tcctgcattg ttcctcacgt cctttctgtt gtttcatcac atcttttaaa
     7081 gtctggcatg acataccaaa cacatatttt gaaaaatctt ctaactgttt ttcttcatag
     7141 tcggcaaggt tcagttgctt catttcatgc cacaccttac gcttgtaaga gggcaggtct
     7201 gaaatgtttt cacaaccaac ttttgcctgc atatctctaa aaatatcgtt cattacatca
     7261 ctccaatctg aaatttaggt aagaaaaaag cagtcaatcc taagactaac tgctttgtgt
     7321 gtatggatat gaagttgtca aactgggatt tatctggcag acttgtttat ccactcatca
     7381 agaaatttca tttgttcttc cgtgtgaaac caatgctctc cgtttctcat aacagtaagt
     7441 gttgcattag tttgttttgt aaattcagat atagttgcat aagaagtcaa gttatctttc
     7501 tcaccaaata aaatatttgt tggaattttc catgtagttg gattttcttt tacataacag
     7561 agatattccc atgagagtgt ttctccaaaa gttgttttaa tttctttttt atctcgaagt
     7621 tcatcttctg taacatttgc ccaatacatc atatctgcaa tcagtttttc catattcaca
     7681 ataggtgaaa taaaatatgc tttttctatt tgcttatctg ataaggcgtt catagcgaaa
     7741 taggcaccta tgctatttgc aactatttga acagatttgt aatgtttgca aatcaaatca
     7801 aaaagtgatg gaaattcttc ttttgcttcc catggatatt gtgcagtata gttgagtcca
     7861 accacatcac aatcactaaa aagaggtttg taatgtgaag actcctctgc atttccacct
     7921 ttaccatgta tataaataac tactttatcc ataatagttg tcctcctcaa cttcaaattt
     7981 gtcgctttct tcatatccac attatatcat tctcatatca gcaccacaat aaaattttac
     8041 gcaccgataa tcttattgaa cagctctaac agtacgtctt tcacaccgcc agcattgaat
     8101 accaatccaa cggcaacaag ggcaactacc aagaagccga taagtttaga aaactcacgt
     8161 ttgaacccta agtagatacc aataaccaca atcgccataa gcactaagct ctgggcgttg
     8221 cttaaaaacc atgtgtatat ggataggtaa gttcctgata ttataaatat ctcaaacttt
     8281 tccccccatt cacacggagc gtgcgacttt caacgcactc cgcgttccat cgtgtttata
     8341 ttgaaaagta tttatgtagt agacctatat caagtttaat tcttgattgc cagctaatac
     8401 tctatattta tttagtctta tgagctggac tttatttagc ttcaatagag ctatatattt
     8461 ttgtattgtt tcttctgttt tagcgtgtat tagacggtgt accgcctctt ttaccaaaat
     8521 atgattttta taatcgtcat ttccaccagc tccagttggt acaatatgat gacaatgtat
     8581 ttcattagct tgttgaaatt cttccagagt tatagcacat tttccatgtt gagcagaaaa
     8641 tagcgaaatt cgattgtctg cgtattctat tgtgcgattt tccaaagata atttcattaa
     8701 ctggtacatc agtaatttat caacacttag attggtgtgg ataaattttc ttccctctag
     8761 agtgtagcaa ttcgctaaat acacctttgc cattggtttt ctgtgttgga tatatccaac
     8821 agggaaaata ggttcgccct cgccagcaac ataccggagc attttagatt taccatatct
     8881 tgttgcctcc tttgtcgtaa gttgtctgcc tgtttttctc aatctgcccc ttttgtttgt
     8941 acctaatctg ttagtgaaga tagccattac agctctattc agtttcatac aatccagatt
     9001 tacattcgtt gcaatctgat aataattttg catacctaat accattgaat tataaagtct
     9061 tgtttcttct aattccgttt tcccttttga gggagaagca attttctttg cctgtgatac
     9121 cagcttgtct ttttggtttt tgaattgttt atcacttata tgtgatttaa caactaactt
     9181 ctttcttctc ggtatcattt taatcttgaa accaagaaag tcggagtatc tttttctaac
     9241 atttacaatt cttgtttttt cttgtgatac ttccaatttt aatcgttgtt caatccattg
     9301 agtgattgct attttggctt tttcagcact ttccttgtag cggcagaaaa ctctaaaatc
     9361 atctgcataa cgaaccatat acatttcctt taactttgtt ttcttcattt ctttgtaagc
     9421 gttgctttta catgggctac cgcttttatt gatatgaacc acatagtttt ttgtaacagg
     9481 attttcctgc cattggcttt ctacccaatg gtctaactca ttaagaacaa tgtttgctag
     9541 tagtggggat attattcccc cttgcggagt tcctttctct ggatatgtta ttgtaccgtt
     9601 agtcattttg attggagctt ttaatatcct ttttaaaatg aatatcaagt gcttgtcccg
     9661 tattcccata gcccatatct gttttatcag cttggaatgg ttcacgttgt caaagaaacc
     9721 cttaatatca aattcaatca cataatgtag ctggctgatt tgtaggcggt tataggttgc
     9781 ttttattgca ttttcaactg acctattagg tctgaaacca tagctgtttt cactaaattt
     9841 agcttcgcag attggttcta atacttgttt tatacattgt tgtataagcc tgtcccacat
     9901 acaaggaata cctagaggtc tggttttacc atttggtttt ggtatttctt ttcgcctcac
     9961 tggcttaggt cgatacccat gtggactgcc tgcaacaatg tatcttactt ttttgacaac
    10021 tgtttctgct ggcaatttgc caatatcttt aattattgta ccgtctgttc ctgctgtttt
    10081 actgcctgca tttgctttga tatttcgata tgccagtaat atattttctc tcgataggat
    10141 aatatccata agatgagtga aaatatctcc attcttactt ttggcatata aatcatcaaa
    10201 ggtgttttgc atttcgtaat attcagcgtg tcttaaatct tctacacaca atgtttcatt
    10261 tttattttta ggcataaggc ttcactcctt tgcttgaaat tgagtgagct tttttatctt
    10321 actcgtaatc cttattgaaa caacataata cttttctaat atatacacga cttgagggca
    10381 ttcctccatt ctcattacaa gaatttctta ggttcgccct ctgcttttca gcattagttc
    10441 aactgcttat tgttcttcgt cagtttattc tcttgatgag aacccaatac ctttcctcgt
    10501 tccgataatc ttatctatac atatatatcc ttaggtcact agctataagc ctgctggctt
    10561 gataccgcct gtaacgttat atggcatttc ataacctata ttcttacttt gccacaccag
    10621 ctacgatttc tcgtaccttt gcatttctac aaagttcaaa tttagacccg tacattcgct
    10681 gtttcgtcag ttccttatag aacattctaa ccatagatat tttatagacc cccgacctgt
    10741 tggatacgct acccgcctct ggatagtttt cgatagaaga tatatgttca tcaactatta
    10801 cggtatcttt cagccgtctt taccgagctt caaacattct gagtttcctc atacaatgct
    10861 tgtcggaggc tcagggcagg cgtttcagag cgttacttca tcattccacc tgtcagatta
    10921 tcagttctcc taataaccaa actttttcgt tcttttatta gtgccttagc ttttcactaa
    10981 ggaacgagtc gcacataaat tttgtccgaa attcatttaa ttgtcctctc tttctttgat
    11041 ttcttccata catctgtcag cttctttgcc gacagttaaa atgcacatca agactacacc
    11101 gatacccatt ccaaatgata ccagcaataa gtctttcaat aattcccaca ttttttcatc
    11161 actcctttac gttggcaacc atttcttcaa ccgtggctgt ctgttgcttg attatctggt
    11221 agtgtcgttc ggtcagtttt gcctgctttt caatcgtttc cagatactcc gtctgattgt
    11281 ccttgtctat ctgcttcaac atcttcaagg tcggtgcaac ctgcctttgt agccagttga
    11341 gtgtacgctc cagcgtgtaa ggttctggct gtgttgtcag ctttacgggc ggtcgcccct
    11401 cgccaatgaa ccaagcccag cgtacattgg ttttccagtc gctttttcgc ttgtctggtt
    11461 ctttatcagc gaagcggata tattgattga taatagaaaa tgctgtcagt tctgcgtcat
    11521 aatgtgtcag cagttccaca acagcataat aggcacgttc atttttcaaa cgtatctcaa
    11581 atctattttt tatggggact tcctcaatcg gtatgccaag tttggcgtac tgttcgtaat
    11641 tcttttcata acagcagaag tacacttcac ttgaaaatga accaatatac agcgtgtgtc
    11701 ccataccgta tttatccgtt tcattatgct ttaccagctc tccagaacca tagcttttga
    11761 aagaacggaa tttagaaata cattcctcat tctcgcattt tcgtatcagc tctggaatat
    11821 caagtaagcc cgttcggtca ttgattgcca aatcaagacg cttcattaca ccgccctcaa
    11881 ccaatgcgtc cgtgaagaag tcataccaac ttcgctcttg tgccagcaga tagctttcaa
    11941 actggcgaca gcctttgcct ttcagttcca gcaggacacc tttttcttcg tcctgcgata
    12001 catacacaaa cacatctccg atatggtaat gctcggtgta tttgtaatgt ccgtaatctt
    12061 cgtgaagcat atagccgatg ttgagcttca atatatcctt gatgatatgc tttatatcca
    12121 ttgtgggaaa acggatacgc acataatcaa acagcaggaa catgggattg ttgggattga
    12181 atttttccac cgcttctaaa agcgtatgtt tcatgtcctc ggtcagcgtt acctttcctg
    12241 cttcgatacg gctcaaatgt tcacggctga tatttgccat gattgcaagc cttgtctgcg
    12301 atacgccgta tgcacagcgt ttttctttca aggcggttaa aaaatcagtt tcattcagtt
    12361 gtcatacccc caatcgtgat aattttgtga tattgtgata atttcaaggc tttatcacaa
    12421 aaggcgaaat gtacgaaaaa cccttatttc atgcggtctt gcaaaggttt ttgtggaagt
    12481 ttccgccgta attgtgcccc tctgttagat aatgaggggc ttgtatctgc tggcgtggct
    12541 aacgccacac cagcaacggc tagtccgtgc cgtcgccttt cgcttcgcac gtcgccgtcc
    12601 cgtcctgcct tgcgtatgca agtgagccta tggtctgcaa aaaatcatgt ccttttggaa
    12661 ctaagggtgt gtaaaattca cttatgacgc ttgttcccac atcacaatag ccacgtccct
    12721 tgatacgctt ctggaaaaac tgttttttca catcagaact gaacagcata ccgtaaccta
    12781 attcgctgat acgccccaag ccaacacgga aattaaaatt atctctgata ccgtccgaga
    12841 aatattttgc gtctggacgc tggcaggcaa caataaggaa atatcctgct tgtcgtccta
    12901 acatgacgat tttctttagc tggctaagta ggctcacact ttcttttgtc cccagcattt
    12961 caaaaaacgc cacatattca tcaaagataa gaaagcaggg cggtaaacca agataggcat
    13021 agttttcgcc cgttttatag tctgggtgtc gcttcatttc ttcgcttctt tgtaccatgc
    13081 cctcataaaa ggcattgaca caatcaatca tttcttcttt ggtgtgatac acatttccca
    13141 ttaccgtacc taaatctgca aggtcagcgt tcttcgggtc taatatgtaa agaacagcgt
    13201 tcgtatgtaa cagagcttca ataagcgtca gcagaaaata cgttttaccg ccaccagtcc
    13261 caccagcaat cagagcgtga gggagtgcgt catattccca tacaagattt ttcatcagcc
    13321 taagacagcc gttttctgct ttcacttcat caatggtaat gcggttcgct atcatatcat
    13381 aaagcatggt gtattcgata taaccgtcat gtaacgtctt gtcggtcagc tcacaataca
    13441 agccactttc caatttatcc tctaaccgta agagctggtc ttgatatttt cctaatgtaa
    13501 tttcacagcg gatatgaagc agtccttttt ccatttgata ataaatcttt ggaaaccaga
    13561 tgattttttc ccttgatctg ctttgcaggt cagtaaaaaa accgctgtct tgtacggtgt
    13621 ctgcttcata ccacttgttt tccagtatca ttcgtgccag cttttgacgg tgcaggagct
    13681 tcttgaaact gtcataacag aagcggtaat acagaaaagc gaccaatgca caaacaccag
    13741 tcgctatcag tagggttatg aagttgtaag tggaaagcgt caaaccgttg tctaataagc
    13801 tgaaatgctc ccaatcggta cgcatgagct gtttcatatt cagtagcagg ataaccgcaa
    13861 tgaatacaaa cagaagcgtc cctatggaaa agtggtagac aaggttcttg tcgctggctc
    13921 tgatacggtg tcctttgttt aaaatcattt tcataaatca atcactcctt tctgggtacg
    13981 aaaaaagcag tcaatcctaa gactaactgc tttgtgtgta tggatatgaa gttgtcaaac
    14041 tgggatttat tgttcttaac ttttctgtcg ttataaagct gacttacgca cattaacctc
    14101 tgcacagacc agcccagcta tatgctccgc tgtttcattt gtcagtacaa agagcattcc
    14161 gtcattacgg cattttttat gtgtgtcgcc attcaaggca taaacaaaac cttttacaca
    14221 ggtatcacta caggcattag ggtcaatcag tctcttgcag tcacgagaag tagtcatttt
    14281 cttttgcata tcagcaggaa gcgaagcgat tatatcctca tactttccag cgttgtctcc
    14341 gtaaatccgt gcaaaaacac ctgtcttgcg aaacacccaa ttcattactg ttttcttctc
    14401 tagttggtat gaagcggtgt aaccgctttt tgcttccttt attacaaggt cacagccctg
    14461 ctcaataagt ttggtattaa gtttctcgac aaacgcttgg tgttctggcg cgattgccga
    14521 tagaaattct ttgaatgaac ctttttcttt tgccattatc tttttcctcc acaaatttca
    14581 atttatccgt ttcttcatac acatattata acagttgctt atatccgcta caatgaaaat
    14641 ctagtttgcc agcttatttc tttggttgcc cctgcggtgc atggttctgt ggattgccta
    14701 cttgaccgcc tttattcttc aagataatat cgtcagcctt gatataccag tccacattga
    14761 cacctcgata gctggcgttt gctaccgtat cggctacggg attgacaagc tctacctctg
    14821 cattatacgg atagtccttg acgggaatat cagcaggaat tgatacttgt atcatacgtt
    14881 cctgcacact acatttcaaa tcataggttc ttcgtgtgat ttcttcactt gttgaaccgt
    14941 cgtcattttc tgctcgcacc tcatgtctga gggcagagaa cttcaataca ccgaaagttt
    15001 tttccttgtc taatacaata ccgtttgcta atctcataat catttacccc cttacttgct
    15061 ttcctcggct ggcaacatat catcagccgt taagatatag tttgtgaacc ctcgtccttt
    15121 gaccgcttta ccgtctgcgg taatctttgg attgataagt tttactcgct gttctggttt
    15181 gaaatgcttt tcgcctgctt tcgctggaat ggttacgata atatcatcag ccctttgtac
    15241 gtcagagtac aagttgtagc ttcttgatac gggcgtaaag cgtccgttaa tgcgttgctg
    15301 ttcaatcttg ttttcgccag caaattccaa gtttccaaaa gtctgtgcca tgttcggcac
    15361 tacatatttt aattccatag tgttttacct cgtttctttc ttctttttta agtttgttga
    15421 ttgtgctttc ttattctggg aattagggaa ataccagtaa tcccccagat agtaaagggt
    15481 aaatcaatgc ttcgcaccct tgactatccg tgtgcttgca ggttgttggc agacaagcca
    15541 gaataagtaa acgtctgccg tttgacagct tcggcagaga gcgtgatttt tcttttctca
    15601 taccgttacc gccttatgat ttcttcattt tacggcgttt gtaaagatat gttccagcca
    15661 gtccaccagc agatgtaagc aacattacca gaaatgccat gacatttgtg ttgtcgcccg
    15721 ttttaggact gtctgttggt ctgctcggtt tttctggtgt tgttggcgtt tcgggtttct
    15781 ctgggacttc tggtttttcc ttaaaagtaa tcgtctgtcc ctcgtcctca atgtccttat
    15841 gctcagtaac ttttttcggc tcgtttggat tgcttaaatc atacaattct tcaaaagtta
    15901 caagctgttt gccgtcaaga gaagtagcgt caaaggtaaa ggtaacttcc actttcatag
    15961 tttcgctgtc agcgataaac gtataatcac tttccacacg ttttccattt ataagaagct
    16021 ctgcattttc ttctttcaac atctgccagc ccacaagttt atactttgta ccaacttcca
    16081 agccctctaa ggttacagta tcaatgattg ttacgtcttt tcctgcttca agctctttgt
    16141 taccgtcctt atcggtagca gttgtatgta ttttgatgat acgttctgta ataagtaccg
    16201 tctgcccgtc gtcctcaata tctttatgct ccgcaacttt catgggttcg tctggattgc
    16261 ttaagtcgta taattcctcg aatgttacca gatttttacc gccaagctcg gacgcattga
    16321 atgtataaga aatttccact ttcatttctt catcatcagc aacaaaggta taatcgcttt
    16381 ctacacgttt cccgtcaatg ataatctcgg cgttttcttc ctttaacatc tgccagcctt
    16441 ttaactgata cttagtgcct tttgtaagtc cgtctaattt gacagtatca acgattgtta
    16501 cctcttttcc tgcaaggatt gtcttttcgc cgtctttact ggttgctgtg gtatggatag
    16561 tgatttcctt ttcgtattca tcagtcaaag tccctaaatc aatcacaaga ttatttcttg
    16621 ataccacgat ttcaaatggt gggataagtt ccatgccttt gttgctgtca gagcgtaatt
    16681 cttcaataat gtaagtatcg taaggcaacg cacctttgct gtcgtctggt tcagaagttc
    16741 caaaccatac accgtcctcg cttgttttgc cagcgttggt attgtgcttg tgcgaagccc
    16801 attttgaaga agtggagaat tgcccattat cgtcagttac cacaacatga ctttcgcccg
    16861 ttgtcttgct tgtgatccta aagggaacat cagcaagacg cttgtgtgtg cctgcaccga
    16921 ttttcacgcc ctcaatatcc ccacgcttaa tctgattgta gatagagtgg gcttcgtctg
    16981 ttaagtccac gatttttccg ttttctgtga ttgtaaaatc aatcggcttc gcaccgtctg
    17041 tcaagtaacc gtttggagct tgtttctcaa cgatacggaa attcccgtaa ggcaaaaggt
    17101 cagcagaagt agaagcgata ccctcaatat ccgtatggat tgtttttaca acttcgtttt
    17161 tcttgtatat cttgccctca accaataccg cattatcatt taaggaaatg atgtcaaagg
    17221 cagtatcttt caaagtggca cttccttgtg gtttggtatc tttggtttct aaatctcgtt
    17281 tctgaatttt gacaccgcca cgaatgactt tatctgatac ggaaaactgg ttacttccag
    17341 ttaatacggc aaggtcgccg tcctcggtaa tctgcgtaag gtataaggct tttatctgtt
    17401 cttcactacc attcgcctgc atatatgcac cgtctagtaa gtagcccgtt ggcgatttcg
    17461 tttcttcgac cgttagtgta ccaagaggta aaaacgcttt tccgtcctgc atatagaagc
    17521 tatcgccaga taccttgtat gcgtctgcta attttgtgat gtaatgggtt gtcccgttac
    17581 tgtctgtttc agcggttgtc tttgtaaccc atgtacgagt agcttcggca ggaagattgt
    17641 ctttattgta gaagcctgca taatatttcc atgtaaactc cgccccctct aaagaagcgt
    17701 tcccttgtgg gttggctttc tgtgtttcca tgtcaatttt aaaaagctca atcaaagtat
    17761 cggtaacttt tggcgtatca gatactttca aagtcgctgt ctttccagct tcgactttta
    17821 aggaatacac cgtagtatca actttatacc ccaatggtgc agataattct ttgatatata
    17881 cggtagtagc tttgacttct gcaacctctg tatttccgct gtcgtcagtc gtaagggtag
    17941 caatctgttt ggtgcaatcc ttatcggaaa agacaccgta agtcgcacca gcgatagagt
    18001 aatttccgtt accgttagta atgctggtat tattggaagt cttttgaagt ttggcatttc
    18061 cgacatttaa cttcgcccag aattgcccta attcctgccc ctcgccagag tagatataac
    18121 cgccgcattc atagcgtcct ttgttttctt tcacaaaggc ttttgcacca gcaaacactt
    18181 catcttgtgt tgcctttggt atttcatcat aagaagctcg cacgttatca cattgccagc
    18241 ctaagtgaac actcaatctt tgccacacaa caagctgtct taaaaggtag gcgtgatttt
    18301 tgcttatccc attgtggttg tcggtgtatt gcttcacata ttcgatagat aaggcaacat
    18361 cacttatctg gtcggcactc atacgggagc ttgcgtcagc tctggtcttg tagccgttct
    18421 taaaatctgt gttaatatcg atacaataag cgtcctcgcc ctcaacggtc aaatgcccct
    18481 cgttaaaggt agaaccaatc gaaccgtcat tcattacttt ttcaacgata ccgacacgtt
    18541 cttcactttc cgtccagtat tgcttgcttt ctgcatggac aagtgtagtc ggtaaagcag
    18601 taacgacagt cgcaaaggca agtacgcccg tacacaatcg tttcaatatc tttttcataa
    18661 ttctaaatgc tcctttcatt ttgggtaata aaatagccgt ccataatgaa cggctggaaa
    18721 tagaaaagga acgtcaattt aggcgttcca tagtctatga aatattcaat ttttttcttg
    18781 cttcaatact gatgtgccgt accatatctt tgcatatcta ggaaaacttg cgtatcaagg
    18841 ctcattcatt ggtagtaaat tagtagtaaa ttgatgtgtt tgtttccttg aaaatgctga
    18901 tagatactat gttttagcgg ataatcaact ttttagtatg ttttttgcat aaaaattgag
    18961 tagatagtag ttttattgta cttaatgatt gtatttaagt gattagattt gaaattgaat
    19021 aatgtagata ataaaaaaat attaacagaa cttaataaac tccattaata tttttttatt
    19081 atttttgaaa ctgtgataat tatcacggaa gcatagaaaa taaagtgtta agatttaaac
    19141 aagatagtaa gtaagtaaaa ctaaaatata agaaaggtga taaaaatgaa actaaaatta
    19201 gattcatcta agttcaatga aggtctgaat tatctaaaaa aagactacaa gatatttgca
    19261 ccagttttaa taccatttaa aggtactttt tcagatactg atgttataag atacaaggag
    19321 gtaaacgctt ttgaagaaat ggaatttgct aagaaagcaa acttttcacc aaaagatgtt
    19381 gtacttccca taaatcaagt attgttttat tttactgaaa aagagtttaa ggaaagcgat
    19441 ttagacaaca agaaaatttt aattttctta agagcttgtg acataaatgg tataaaaaga
    19501 cttgatgaaa tatatctaaa taatggtgaa gaaaaagatt atttttataa aaatatcaga
    19561 gataaagtaa agtttgcact tgtagggtgt aaggaaagtt ttagaaactg tttttgtgtg
    19621 agcatggatt ctaacaaaac tgataattat tcaattggat taaatataga aggagataca
    19681 ctgtacttag atataaagga tagtgaattt gaagtattta atggagaagc aactgaattt
    19741 aatgttgatt atgtaactga taatttaata tctgtagatg tgccagagaa tattgattca
    19801 aatgaaatga taggaaattc aatatgggat gaatatgata caagatgtat aggatgtggt
    19861 agatgtaatt ttgtatgtcc aacatgttca tgttttacaa tgcaggatat tttctataag
    19921 gaaaacgaaa atgtaggtga gagaagaagg gtttgggctt cttgtcaagt agatggatat
    19981 acagatatag caggtggaaa ttcttttaga aaaaaacaag gcgaaagaat gagatttaaa
    20041 actatgcata aaatacatga ttttaaaaaa agattcggat atcatatgtg tgttggctgt
    20101 ggaagatgtg atgatgcctg tccgcaatac atatcatttt caaagtgtat agaaaaaata
    20161 aatgatttag taattagtaa ggaggaagta taaatgaacc catatatacc agtaagtgca
    20221 aaaatactag atatagttaa acatactgat aaagaatgga cttttagagt aaactgcaat
    20281 acaaaaggtg tattgcctgg aaagttttat gagatatcta tacctaaata cggtgaaagt
    20341 ccaatatcag tatcaggata tggagaggat tatattgact ttacaataag aaatgtagga
    20401 aaagtaacta gcgagttgtt taattataaa gaaggagatt ctttttttat aagaggtcct
    20461 tatggaaatg gatttgatgt atccctttat gaaggaagag aaattgtagt agtggcagga
    20521 ggaagtgcat tagcccctgt aagaggaata gttgaatatt tttataataa taaagaaaaa
    20581 tgcgaaaagt ttaaattaat agtaggattt aaatcaccaa aagatatttt atttgcagat
    20641 gatttaaaga gatggagtaa aaaacttgat attttagtta ctgtagatgg agcagaggaa
    20701 ggatatagtg gtaatgtagg attagtaaca aaatacatac cagaattgga tatgaaagat
    20761 ataaacaata cttctatagt tgtagttgga cctccaatga tgatgaaatt tacagtagaa
    20821 gaatttttaa aaagagattt agatgaaaag aacatttggg tttcttatga aagaaaaatg
    20881 tgttgtggaa taggtaaatg tggacattgt aaaatggatg atacttacat atgtttagat
    20941 ggaccagtat ttgattattc atatgctaaa aatcttattg attagggggt gaagtttgtg
    21001 ataagagatt taaataccaa aaaagtaatg aaaaatgctt atagaattac aaaaacaaaa
    21061 tatgagactt ctttaagagt tagagtacct ggaggtctta ttgaccctga gagtttagct
    21121 gtagtttcta aaattgcaag tgagtacggc aatggacagg tgcacataac tacaagacag
    21181 gggtttgaaa tattaggaat aaatatggaa gatatggaag aaataaataa aattatacaa
    21241 cctgttattg aaaaaatgaa tataaatcaa agtgagaaaa acgcgggtta tccagcagca
    21301 ggtacaagaa atatagcagc ttgtatagga aataaagtct gtccaaaggc tcaatataat
    21361 acaactgaat ttgctaaaag aatagaaaaa gctatatttc caaatgattt acattttaaa
    21421 gtggctttaa ctggttgtcc aaatgattgt ataaaagcta gaatgaatga ctttggaatt
    21481 attggtatgg ctttaccaat atatgaaaag gatagatgtg taaattgtgg agcatgtgta
    21541 aaaaaatgtt ctaagatatc tgttggagca ttaaaaacag aaaataataa ggtggtaaga
    21601 gataaagata agtgtatagg ctgtggagag tgtgtactaa attgcccaac aaatgcatgg
    21661 actagagatg agaaaaaata ctatagatta gctataatgg gtagaacagg aaagaaaaac
    21721 cctagactag cagaagattt tcttttatgg gtagatgagg acagcataat aaagataata
    21781 cttaatactt atagatatgt agaaaagtat atagataaag atgcaccagg aggaaaagaa
    21841 catataggat atataattga tagaacagga tttatggaat ttaaaaaatg ggcacttgaa
    21901 ggtgtagagc ttccagaaat aactaaaatg tacgaaaaca tatactggag tggaattaaa
    21961 tatctataaa ttgagtaaaa catatatttg aataaattaa aatttaatag aaggaagagg
    22021 gagttatgca taaagaaact ttggataaat taactaatgc agctataaat aagataaatt
    22081 tattaaacac aagtaaagta aaatatttgg tatcttctgc gtttgcagga ctttatgtag
    22141 gtataggtat acttttgata tttactattg gaggactttt gactgatgca ggttcaccta
    22201 tgactaagat agtaatgggg ttatcatttg caatagcact aagtttggtt ataatgacgg
    22261 gaacagagtt atttactgga aacaatatgg ttatgtctgc tggtatgtta aataaaggtg
    22321 taagtataaa agatacttct aaaatatggg catacagttg ggtaggtaat ttaatcggag
    22381 ctttggtttt aggcattata tttgtaggaa ctggtctagt agacaaagga cctgtagctg
    22441 aattttttgc aaatacagca gctagtaaag catccatgcc atttacagct cttttcttta
    22501 gagggatttt atgtaatatt ctagtatgtg tatcagtgtt gtgttcgttt agaactaata
    22561 gtgatactgc aaaaataatt atgatactct tatgtttatt tgcttttata acatcaggtt
    22621 ttgagcatag tgtagcaaat atgacaattt atagtgtttc attattttca ccaacaataa
    22681 gtacagttac tattggagga gctatttata atttagtagc tgtaacactt ggaaatattg
    22741 ttggaggagc tttatttatg ggtcttggaa cttatatatt aggtaaagaa aagcttaatt
    22801 aaatttattg tataaattat aactctcttg ataaatacat ttttagtatt gttgagagag
    22861 ttatttttat atttttgaaa aaatatttga aacaaaaaaa atatgggagc atacgaaaat
    22921 atatcggtta tatatataaa aaatacgttt atatatgaat tatatagttc taaatataaa
    22981 aaaagttaaa gtcctaccgt aaaaaagcgc attttgaata agataacaaa tatgataaaa
    23041 ttggtcttgt tgaaattttt aggggggata tgatgacaaa gaaactagta atatcaaaaa
    23101 caaagttgtt tatttttata atagcattta tttctatatt tatatattta tttggaagta
    23161 aaaatacact tataggtgta ggtatagtaa cagctatgct aacactcttg gaaagagatt
    23221 taactatttc tccaattaaa aatttactga agtatttagc aataaatata atcttaggaa
    23281 tactatcatt ttttgcagta caaaatatgt atttaggagt tttattaaat tttatagcat
    23341 tatttataat agggtatgta tttagttatg acttaaaaag agcagtatat gtaccctttg
    23401 gtctaatgta tatatttatg gtaagtatac ctgtaggaaa aagtgaattt cctatgagat
    23461 tgtcggcttt agctgttgga gcagttataa taatgattgc tcaatttgtt atgaatagaa
    23521 ataggatgaa aaatgtcgga gataaagaac tgatatctat atgtgatgaa ttattagaaa
    23581 aaattagcct tttaaaaaat actattaatg atgatagttc tataattaaa tctagtatga
    23641 gaaaaataga tagctgtaat tatagaataa attctatatc taaaaatttg aaaatggtta
    23701 tttttgataa tagaaaagat gacttttata tcagtattag aggtatagac ataatgaata
    23761 ttttattttc attagaaaga ataagtttaa tcctggaaag atacaaaaag gatagtaaag
    23821 aatttgaaga tgaagatata aaaaatattt tagaagaaag tagcataaat agtaaatcag
    23881 atgttttagt tgtagcaacg aaagaaataa actatattaa gatgtgttta gaaaataaag
    23941 ataccataac tgataaagag atattaggat ttagagatta tgttattgct caggatacta
    24001 aaaatataaa tttaaaagaa atttatagtg ttttagaaaa tttatatgag tttttagtag
    24061 attataaaaa agtaaaatca agagacgaaa agaaagctga aagaaaatca aagatacctc
    24121 atgaatttaa acgactatca atatataaga aaaactttaa tttaaattct ataagatttt
    24181 cttatgcagt aaagattgct ttagctacag ctgtagcggg ttttattatg gattatttcc
    24241 atttaagaga tggaagatgg ataatgttaa ctgtcttttc attaacacaa ccttacgcag
    24301 aaaattgtat acaaaggtct agaaaaagaa tagaaggtac atttatagga gctgtaatat
    24361 ttattgtatt attttcaata ataaaagata gtacattaag gtcgttaata gttcttttag
    24421 cagggtatat aaactcttat gtagttgatt atagaaaatt aatggtatgt gtaactgttt
    24481 cagctcttgg ttcagcagta gttatgggag acccaaatgt tttaacaata agcaggattt
    24541 catatgtagc attaggagca attattgcat taatagtaaa taagtttata ttaccatatg
    24601 atgctaaaac aggctatcaa catgttatag aaatgtataa aggaatagtt aaaaatataa
    24661 ttgatgaagt caataagtct atagaaaatg tagctgatgt ttactatata aaaaatttat
    24721 tattgatacc atcacttatt gaagatagac ttatgttgat aaatgctata tataaagata
    24781 aacatcaaga ggatttttta gaaaatcaga aattactaat aagtaatatg tataatctat
    24841 atataaatgt taataaaaat aaaataaaag atgaagatgt tgaaaaaata ttgagagata
    24901 ccaattatat ttcaaattac aatgtagata agtatgatga aggaagaagt gtaattttag
    24961 agagtattgt aaatactaaa agtctaggtg acaagataat atgtctaaac ttactgcaaa
    25021 ctctaaatgg tgtcaaagaa atgtatagaa taagtaatat a
//
LOCUS       DACULQ010000041        24972 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.041, whole genome shotgun sequence.
ACCESSION   DACULQ010000041 DACULQ010000000
VERSION     DACULQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24972
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.041"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            112..780
                     /locus_tag="JA154_06685"
     CDS             112..780
                     /locus_tag="JA154_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995550.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            782..1267
                     /locus_tag="JA154_06690"
     CDS             782..1267
                     /locus_tag="JA154_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995551.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1328..2449
                     /locus_tag="JA154_06695"
     CDS             1328..2449
                     /locus_tag="JA154_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7995552.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2730..3920
                     /locus_tag="JA154_06700"
     CDS             2730..3920
                     /locus_tag="JA154_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAT7995553.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4054..4309
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4368..5369
                     /locus_tag="JA154_06705"
     CDS             4368..5369
                     /locus_tag="JA154_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAT7995554.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5473..5862
                     /locus_tag="JA154_06710"
     CDS             5473..5862
                     /locus_tag="JA154_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995555.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            6023..7231
                     /locus_tag="JA154_06715"
     CDS             6023..7231
                     /locus_tag="JA154_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAT7995556.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            7607..10651
                     /locus_tag="JA154_06720"
     CDS             7607..10651
                     /locus_tag="JA154_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HAT7995557.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTPVHAESKQYWTESKERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKIRTDASTRMSADQISDVA
                     LSIEYVKQYTDSHSGISNNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGARAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDENGNTDVVEVKAGTVYIKELSAPAGYKVDKT
                     VYPLTIKAGETATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGVEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTVAETDSDGATHYITKLADAYKVSGDSFYMQGVKAVLPL
                     GTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYVTQITEDGDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIIESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLDGLEKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFVADEEEMKVEISYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENIELLINGKRVESDYTFTADSETMKVEVVFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKPETPPTPEKPS
                     RPSDSPKTGDSTNVMAFVVMLLASAGGLAGIFFYKRRKMKNS"
     gene            10664..10864
                     /locus_tag="JA154_06725"
     CDS             10664..10864
                     /locus_tag="JA154_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995558.1"
                     /translation="MRKEKSRSPPKRSNGRLPLILACLPPTCKNTDSQGCVSIDFTLY
                     YLGDCWYFPNRQNKKSQSIYQK"
     gene            10886..11212
                     /locus_tag="JA154_06730"
     CDS             10886..11212
                     /locus_tag="JA154_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995559.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKKFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            11228..11611
                     /locus_tag="JA154_06735"
     CDS             11228..11611
                     /locus_tag="JA154_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995560.1"
                     /translation="MRLSNGFVIDREKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGSPQNNNSQGQQKK"
     gene            11695..12333
                     /locus_tag="JA154_06740"
     CDS             11695..12333
                     /locus_tag="JA154_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7995561.1"
                     /translation="MKYIFLHGLGQTSFSWNKTLEVMSDKWDILCPNLADWLYNERPC
                     YETLYKALERCCKQFNEPLTLCGLSLGGILAMQYSIEHSEKVHSLVLIGTQYAMPKKL
                     LKMQNIMFQFMPSSMFSKMGFQKADFISLCKSMIDLDFTHNLKDISCRVLVICGEKDK
                     PNKSASLQLGNLIPYAKITIISGAGHEVNIDAPIKLGNELNVFFNSSHNTER"
     gene            12477..13268
                     /locus_tag="JA154_06745"
     CDS             12477..13268
                     /locus_tag="JA154_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7995562.1"
                     /translation="MTLGEKIQKLRKQNGFSQEELAEKVTVTRQTISKWELNQSEPDL
                     DFIAQLSNIFNVSADYLIKEELTKPDELPFRKKRYQYYFSERSKRTMFVAISIVALIA
                     SIVCLICDYFTSDKLSWSFIAIEAIIAVWLVFLPCMLFKEKVIFKTLIICSIIPIPFL
                     AILALLLKVTTIFTLGSCISVLCIAIMWAIYGIFRKYHQRIFLSLGFSLLLLIFVPIV
                     IVRVTDYFLPQYEIVSKSDVFNGIITFAIALICFGIDYLTQHKNK"
     gene            13338..14720
                     /locus_tag="JA154_06750"
     CDS             13338..14720
                     /locus_tag="JA154_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT7995563.1"
                     /translation="MRKIFNKGHRIRASDKHLVYHYCIGTLLFVFMAVLLLLNSKQLM
                     RTNWEHFSLLQNGLILSPYNFITILIATGVCVLVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHICCEITLGKYQDQ
                     LLRLEDKFESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNSDLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGRLAQERQ
                     VMPEQQGKEN"
     gene            14724..15140
                     /locus_tag="JA154_06755"
     CDS             14724..15140
                     /locus_tag="JA154_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995564.1"
                     /translation="MEIKEINKIIQSEDKDEKVKSVKCICINYGDFLADCEGFVQKRY
                     HDFKCNPKHQFEKKADTILENAIHEKNFMPDLFLIRLNRKQSACNSQIDFVFELLDKS
                     FLETDPIRKSEISEETLNLCLSADVSFIMVYIGMNR"
     gene            15385..16581
                     /locus_tag="JA154_06760"
     CDS             15385..16581
                     /locus_tag="JA154_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAT7995565.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLALASGITREYLNKIESGK
                     MKPSKELLNILHEELARFNPEAPLTMLFDYVKIRFSTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSSDEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMDTIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            16594..16728
                     /locus_tag="JA154_06765"
     CDS             16594..16728
                     /locus_tag="JA154_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995566.1"
                     /translation="MWVLLKDFLLVSMGMGIGVVLMCILNVGKEADYKMKQLEESEDN
                     "
     gene            16729..16950
                     /locus_tag="JA154_06770"
     CDS             16729..16950
                     /locus_tag="JA154_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995567.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            17021..17647
                     /locus_tag="JA154_06775"
     CDS             17021..17647
                     /locus_tag="JA154_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7995568.1"
                     /translation="MKYIMLHGLGQSSESWSDTVKVFNDDFEVLCPNLSDWLSGKTPC
                     YDTLYRTLETYCEQFEEPLNICGLSLGGILAMQYAIEHPEKVNSLVLIGTQYEMPKYL
                     LKMQNLIFRIMPCSAFEKMGFRKTDFINLCKSMMELNFKHELKKISCSVLVVCGDKDK
                     INKKASLELQQQIENAEIAIIENAGHEVNTDNPTLLGQKLNVFFKQYV"
     gene            17736..18032
                     /locus_tag="JA154_06780"
     CDS             17736..18032
                     /locus_tag="JA154_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995569.1"
                     /translation="MNDIFKDMQSKVGCDYISDLPSYKRKVWHEMKRLNLADYEERQL
                     EDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGCWWKREEQLAKKQYHTGLNCR"
     gene            17951..18454
                     /locus_tag="JA154_06785"
     CDS             17951..18454
                     /locus_tag="JA154_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAT7995570.1"
                     /translation="MQETRVLVETRGTTGEETISYWFELPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            18472..18975
                     /locus_tag="JA154_06790"
     CDS             18472..18975
                     /locus_tag="JA154_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAT7995571.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHFGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            19092..19490
                     /locus_tag="JA154_06795"
     CDS             19092..19490
                     /locus_tag="JA154_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7995572.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            19468..21918
                     /locus_tag="JA154_06800"
     CDS             19468..21918
                     /locus_tag="JA154_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAT7995573.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPKKKLNKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQAVILDPKSERGNWKE
                     TLPEIAREINIVNLTSDKDNAGLLDPFVIMKDKEDGATLAKEILTFLTGISTRDGDKF
                     PVLISAISKVSESEHRGLLNVITELRQENTPIANHIANHIASFTNYDFAHLLFSDGTV
                     ENTISLDNQLNIIQVADLVLPDKDTTFNEYTTIELLSVAMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            21918..24080
                     /locus_tag="JA154_06805"
     CDS             21918..24080
                     /locus_tag="JA154_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7995574.1"
                     /translation="MKERIKGAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINVENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTQNGFSSSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTNRVEALV
                     SASPEVEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYLAHRARRMERRLAGAVTAGGVAGAVAGSTISNRKPTRNTATKDSRGNTSTSSMGQ
                     RAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKRGMVQ
                     EQQSRQSGRLEKQEQHKKNIADKRMELQKAQEARQAQRKADGSATTGATRPHERPATA
                     SKPSAEKMQEVKRPAIAQTPKASEPVKTNVIKERPLSSGASDKKATQSAQITHRQNVE
                     KVVSQETRQNYTKDRRTKVQQTTEKTRNLVTKKGQKKK"
     gene            24077..>24972
                     /locus_tag="JA154_06810"
     CDS             24077..>24972
                     /locus_tag="JA154_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAT7995575.1"
                     /translation="MKLKHIALIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKEKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVAHFDNATAQAIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVG"
BASE COUNT         8416 a         4053 c         5449 g         7054 t
ORIGIN      
        1 tttacaataa aaaaataaat acaaaggctt tatcattaag gtattggcat aagttttgct
       61 tattaaacaa gtatactaat aaaattcatt taaacaggag ggagaattta tatgagtaaa
      121 ttagaatcaa caacagaaaa agttgaggta aaatggtatg ggtatgtagc attaatattg
      181 ggagcattat ttttttcagg aatatttaaa gatgcaccag gaccattaaa agtattggat
      241 tttaacaatg tattaggttc ttttggaaca ttgggaacaa ttaatgatgg tgtaggtaca
      301 ttagctgcaa actttagagg tgatggaggt actggccctc gagatggatg gctatatgca
      361 ttgacattaa taccatcagt tatgtttgca cttggagtag ttagagttat agaccactta
      421 gatggaatga aggcagctca aaagcttctt agtccactat taaagccatt attagggtta
      481 cctggatttg caggactaac tttgatagct agtttacagt ctacagatgc agctgcatct
      541 atgactaaag aacttaaaga tgatgggtat atagatgaaa aacaaaaagc tgtattttgt
      601 gcatttcaat tttcaggtgc gtctgcaatt acgaatttct ttgcttctgg agcagcacta
      661 tttccattta taggagatgt accaatattt ataccacttg ctttaatatt aattatgaag
      721 tttgttgggg cgaacttact tagattatat ttaaataaat ttgacaaggg ggaagcttaa
      781 catgagtgag gtagcaaaaa aagaagtaaa agttaaaaaa ggaaatgtga tagatgcgtt
      841 tattggagga gctagaaatg gattccaaat aagtacaaat tcaatggctc caaatgtcat
      901 cttcggtttt gctataattt ctgtgttcaa tttaacaggt cttttggatg taatagggac
      961 aatatttaca cctgtaatga gtatctttgg acttccaggt gttgcagcaa cagctataat
     1021 gacaatattt atatcaatgg gaggagcatc tggagtaatc gcaggtttat ttacagcagg
     1081 tcaaataagt caagcacatg ttgcaattct attgccaatg ttattgcttt caggagcgca
     1141 gatacaattt atggggagac ttttaggaac agctgagtta aaaactaaat attatccaca
     1201 tatgtttgtt atagcaactt taaatggttg tattgcaatg cttataatga attatttcgt
     1261 agtataggta attataataa agatttaata taaatggaaa gtttaaattt taaggaggag
     1321 ctagattatg gaaactaaca aagactatat tgagtatgta cataaagtgt atgattatct
     1381 acacactata ccagaaaaag gatttgaaga atataagaca tcagagtttg ttttaaaaga
     1441 gcttgaaaaa atggggtttg aaaatattaa taaaggaatt gcaggaactg gtattatagt
     1501 aactatagat tctaaaaaag aaggaccagt atttgctata agagcagata tggatgcatt
     1561 ggaatttatc atagatggag aaaagaaaat gatacatgct tgtggtcatg atggtcatac
     1621 ttctatgctt ttaacagctg caaagttaat aaaagagaga aatttagtaa aaagaggtaa
     1681 gttaaaaata ctttttcaac caggtgaaga aaaattatat ggagcattaa gagtaattga
     1741 ttctggtttg ttggatgatg tagaagaaat ggtagggatg catttaagac ctggacaaga
     1801 ttgtaggttg ggaaaggcga tacctgcaat gtaccattgt gctagctgta taatggaatt
     1861 tacgattaaa ggtatcactt gtcatggttc taaaccacat ttaggagtaa atgcagtaga
     1921 tgctgtctgt ttagcagtga atgcaataaa tgcaataaga gtaaatccag ctataccaaa
     1981 ttctataaaa gtaacaaata tatcagttaa gggagaaact tataataata taccagatga
     2041 agcatttatg gctttagata taagggcaga aagtaatgat ataatagaag aattaaaaga
     2101 aaaagcaata catgctgtaa aaactgctgt aagttctata aatgcagaag ctgtactaat
     2161 ttccaaagat ggagtaccag cagcagaatt tgatgaagaa cttgtaaact tggctgaaga
     2221 aactataaaa gatgtattgg gagctgatgc atctataggt atatttaaaa attctggtgg
     2281 agaagatttc cattattata cacaaaaact aaaatgtaaa actacatata ttggattagg
     2341 agctgatgct acaccaggat ttcataatcc aaatgtaaca tttgatacga aagctttaga
     2401 atatggtgta gatatatggt gtagattggt tgaaaagaga ttgggataac atccaaacta
     2461 ataattctac atttaaaatt ataattatag aagttataga aaaagattgc ttatgttaat
     2521 tagaaaataa ttaatatgag caatcttatt ttgatataat gaaactgatt ttggaatatt
     2581 gaaaaaatat gatggaaaag taattatatt ttaaatctat tggacaaaaa agtgatatta
     2641 ttataatatt aagaattgta aaataatacc aaagtgcatc tagtaataga cttaacaatt
     2701 ataaaaattt tattcttagg agggaaaaaa tgagtaagta taattttgac agagtaatag
     2761 atagagtagg aacagattgt gtaaaatggg attttagaac taattgttca acaaaagcac
     2821 aaaaagatgg tttgcctttt tggattgctg atatggattt tgaatgtgca gaaccaataa
     2881 tagaagcatt acgtaaaaaa gttgaacata aaatatttgg atatagttca aatgatagtg
     2941 ataaatactt taatgcagtt tgtgattggt ataaaaggag atttaactgg gaaataaata
     3001 gaaaagatat tatttttagt cctggtgttg tacctgctgt tgctatttta gtaagaatat
     3061 taactaattc taatgaaggt gtaatagtcc aaaaaccagt gtattaccca tttgaagcta
     3121 agataaagag caataatagg gatgttgtaa acaatcctct gatatatgag aatggtactt
     3181 ataggatgga ttatgatgat ttagaagaaa aagctaagtg tagcaacaat aaagtactga
     3241 tactttgtag tcctcacaac cctgttggaa gagtttggag agaagatgaa ttaaaaaagg
     3301 ttgtagaaat atgtaaaaaa tatgacctat ggataatagc tgatgagata cattctgatt
     3361 taattagaaa aggatttaaa catactcctt tacagtcatt atgcccagag tacaaggata
     3421 aaatagttac atgtatagct ccaagtaaaa catttaatct ggcaggaatg cagttgtcta
     3481 atataatagt gaataatgat gaactaaaaa agaaatatca agaagaggtt acagctgttg
     3541 gagttgcaac ttcaccaaat ccctttgcta ttgtagctac aatagcagca tataatgaaa
     3601 gtgaagactg gcttaatgaa ttaaatgatt acttagataa taatatacaa tttattgatg
     3661 aatacttaaa agaaaaccta ccaaaagtaa aacttgttta tccagaagga acatatctag
     3721 catggttaga ttttactgct tatggattaa atgaagtaga acttgaagat ttaatgttta
     3781 aaaaagctaa tgtattattt gatgaaggtt atatatttgg aaaagaagga ataggatttg
     3841 aaagaataaa tgtagcttgt ccacagtctt tattaaaaga atgtatggac agattaaaga
     3901 ctatatttga aaatttataa agtataggta aaaatttata aagtgtttaa aaataaaaaa
     3961 ataataaact tgacaaattt taactaatca cttatcatac tattatgaat aaatagaaaa
     4021 cataagagag ctaaatttta ttgaattaaa ctcttaaaga ctttgaaagg aagagtagtt
     4081 atagaatgta attaaagcga gttggtgatg gtgtgagacc aatatgaagt ctataatgaa
     4141 gaacatcctg gagttgctaa ccgaaatttt aaaagagtag gcttagtcgg aaccaaaccg
     4201 ttatcatatt ggggtgtatc gagtaatatc tgtacattgt gagctgggtt tgaatttatt
     4261 tcaaactaat cagggtggta ccgcggaaaa gcaacctttc gtccctttta tatagggatg
     4321 aagggttttt ttaatggaaa aatatataaa tatagggagt gaatgatatg agcacaataa
     4381 taccaaagga ttataaaagt agtttgaacg taattgatac acaaatagct ataaaaaaat
     4441 taaaagattt ttttgaaatg agactaagta atgaattaaa tcttataaga gtttcttctc
     4501 cgttatttgt attgccagaa acaggtgcaa atgataattt aaatggagaa aaggctgtaa
     4561 gctttgatat accatttatg aataaaaatg cagaaattgt tcaatcactt gcaaaatgga
     4621 aaagattagc ccttaaaaaa tatgggtttg aagttggtag tggtttatat acagatatga
     4681 atgcaataag aaaaaatgaa gaattagaca atatacattc tctttatgta gaccaatggg
     4741 attgggaatt ggttatagat aaaagtagta gaaatgaaaa gactctaaaa gatgttgtaa
     4801 aaagattgta tggagtattt aaagatactg aaatttttgt atgtagtatg tatgaaggta
     4861 taaaagaaat attaccagaa gaaataactt ttataacatc acaagagtta gaagatatgt
     4921 atccagaatt atcaccaaag gaaagagaag ataaaattgt aaaagagaag aaagcagtat
     4981 ttataactca aataggaaaa acattaatat caggagaaaa acatgatgga agaagccctg
     5041 attatgatga ttgggaattg aatggggata tattatttta caatcctgtt ttagattcag
     5101 cattagaatt atcttcaatg ggaattagag ttgatgaaga atctcttgat agacaattga
     5161 aacttgctgg atgtgaagag agaaaagagt ttgactatca taagatgctt ttaaatggcg
     5221 aacttccgta tacaattggt ggaggaattg gtcagtcaag aatatgtatg tatttcttac
     5281 aaaaagctca tataggagaa gtacaagttg gagtatggcc acaggatatg attcaaaatt
     5341 gtagtagtgc aggaattgaa ttattataat ttaaatataa actaattgat aaaatttata
     5401 tctaatatgt tcatcaaatt aattatatcg gttataatgt agaataacct ataattataa
     5461 gaggtgataa atatggagtt tttaaaaata atcattaata tagttttaga tattttaaaa
     5521 aaaattttgg tcagatttaa aaatgctaaa tttggtttgg tttttgtatt tgatttactt
     5581 aaactacctg attttatgac agataaaaga atcaatatag ttgataaaat aaaggtaatt
     5641 tctgtattaa tttttactat atcctacttt gtatctgggg ttgatataat tccagagatg
     5701 attgcagggg catttggttt tatagatgat gctatagttt taatttggag tataggtata
     5761 gtgaatgaag aaattaataa gtatagagtt atcgcaaaaa aagataaaca ttcaaatata
     5821 attgaaaatg tagaattttc tataaaagat gaagaagaat aacaatttat atatgttatt
     5881 ctttttttta ttaacaaaaa atggttaaat tgttgacgga gaggtaaaac tctattatca
     5941 taataagtgg atatccaata tttaactaag tattcaatgt gcaagaatgc ttaatttaaa
     6001 aaagttatgg agggtaaata ttatgaagaa aaaaataggt ttagtgccca aactaatcat
     6061 cggtatcata gtcggtattt taatcggaag ttatgcacca gaaataatag tacaaatatt
     6121 agtaacagtt agtactttat tctcagcatt cctaaaattt gttataccat ttataattat
     6181 aggttttgta acagcaggta tagctgattt agcaacaggt gcaggaaagt tattggggat
     6241 aactacggga atagcatatg gttctacctt aatagcagga ttattatcat ttgcagtatc
     6301 tacattaatc ttcccaaatt ttattgatgc aagtgttgca agtcaaattg gagacccaga
     6361 ggcaggaatg ttagctccaa tattcacaat tccattagag cctatggtag atgtaacagc
     6421 tgcaatcgta tttgcatttg taatgggact tggaatatca gctttaagaa atcatggaaa
     6481 aggagagact ttatttaatt tcttccaaga atttcaagga atcgtaacga aaacgttgtc
     6541 aacagtgatt atacctttat tacctttata catagcagga acttttgcaa atataactat
     6601 agcaggggaa gtatggacaa tattaggggt attctggaaa gtatacttag tagtaatacc
     6661 tttacatttt gtatatatgg catgtcaatt cacagcagca ggtgtatttt caggaaaaaa
     6721 tccagtaaga atgttaaaaa atcaagtacc tggatattta acagctatag gaactcaatc
     6781 ttcagcagca actataccag ttaatgttgg ttgtgcagaa aaaaatggag taagtaagca
     6841 aataagagaa tttgttgtac ctctttgtgc aacaatacat ttatcaggta gtatgatatc
     6901 aataacagga tttacaactg cagttttaat gatgaatggt atgccacatg gaataggtgt
     6961 attattccct tatattgcaa tgttaggtat agcaatggtt gcagcaccag gagcaccagg
     7021 tggggctata atgagtgcac ttcctttcct accaatggtt ggaataccat cagatggtgg
     7081 gcttgcaagt ttaatgatag ctttatactt aactcaagac agttttggta cagcatgtaa
     7141 tgtatcagga gataatgcaa tagcagtagt agtagataaa ataaatgatg gaatgagtaa
     7201 aaaaggaaat aaagaagagc aaagtgctta gttttttaat aatcaaaaat gagttatctc
     7261 aaatagagat agctcatttt ttatggtata aaaaattata aaataaagtg atttttttat
     7321 ttaaaaacac aataaaaatc tgattatccc ctaaaacaca gtatctatca gcattttcaa
     7381 ggaaacaaac acatcaattt actacttatt tactactaac gaatgagcct tgatacgcaa
     7441 gttttcctag atacgcaaag atatggtaca gcacatcagt attgaaacaa gaaaaaattg
     7501 aataacttat agactatgga acgcctaaca tgacgttcct tttctatttt cagccgttct
     7561 tattggacgg ctattttata cccaaaatga aaggagcatt agatttatga aaaagatgtt
     7621 aaaacgattg tgtacgggct tcttagctct tgcaactgtc gttactgctt taccgactac
     7681 acccgttcat gcagaaagta agcaatattg gacggagtca aaagagcgtg tcggtatcgt
     7741 tgaaaaagta atgaatgacg gttcgattgg ttctaccttt aatgagggac atttgaccgt
     7801 tgagggcgag gacgcttact gtatcgacat taacacagat tttaagaatg gttacaaaat
     7861 cagaactgac gcaagcaccc gtatgagtgc cgaccagata agcgacgttg ccttatctat
     7921 cgaatatgtg aaacagtaca cagacagcca cagcggaata agcaacaatc acgcctacct
     7981 tttaagacag cttgtagtct ggcagagatt gagcgtacat cttggctggc aatgcgacaa
     8041 cgtgagagct tcttatgatg aaattccaaa ggcaacgcag gacgaagttt tcgctggtgc
     8101 aagagccttt gtcaaagaaa ataaaggacg ctatgaatgt ggcggttata tctactctgg
     8161 cgaggggcag gaattgggac aattctgggc gaaactgaat gtcggaaatg ctaaacttca
     8221 aaagacttcc aataatacca gcattacaga cggtaacggg aattactcta ttgcaggtgc
     8281 gacatacggt gtcttttctg ataaggactg cacgaaacaa cttgccaccc ttacgactga
     8341 tgaaaacgga aatacagatg ttgtagaggt aaaagcaggc acagtctata tcaaggaatt
     8401 atccgcacca gcaggatata aagtggataa aacagtctat ccattaacaa tcaaagctgg
     8461 cgaaacagcg accttgaacg tatctgatac tccaaaagta acggacactt tgattgaact
     8521 tttcaaaatt gatatggaaa cacagaaaga caatccgcag ggaaacgctt ctttagcagg
     8581 tgtggaattt acatggaagt attatgcagg tttctacaat aaagacaatc tccctgccga
     8641 agctactcgt acatgggtta caaagacagt cgccgaaaca gacagcgacg gggcaaccca
     8701 ctacatcaca aaattagcgg acgcatacaa ggtatctggc gacagcttct atatgcaggg
     8761 agttaaggcg gttcttccac ttggtacatt aactgttgag gaaacgaaag ctccaaacgg
     8821 ctacttgctg gacggtgcat atatgcaggc tggggataag tccgaacaga taaagggatt
     8881 atatgtaaca cagattaccg aggacggcga ccttgccgta ttgagtggaa gtaaccagtt
     8941 ttccgtatcg gacaaggtta tccgtggcgg tgtgaaaatt cagaaacgag atttagaaac
     9001 gggcgatacg aaaccacaag gaagtgcaac attgaaagat actgcatttg acatcatttc
     9061 tttaaatgat aatgcggtat tggttgaggg caagttatac aagaaaaacg aagtggtaaa
     9121 gacaattcgt acagatattg agggtgtcgc ttctacttct gccgacctct taccttatgg
     9181 aaaattccgt atcattgaga gcgaagctcc agacggatat ttgacagacg gtgcgaaacc
     9241 gattgacttt gcaatcacag aaaatggtaa aattgtggac ttaaccgacg aagcccactc
     9301 tatctacaat cagattaagc gtggagatat tgagggtgtg aaaatcggtg caggcacaca
     9361 caagcgtctt gctgatgttc cctttaggat cacaagcaag acgacaggtg aaagtcatgt
     9421 tgttgtaact gatgataacg ggcaattctc cacttctgct gactgggctt ctcataagca
     9481 caataccaac gcaggaaaaa ccagcgagga cggtgtgtgg tttggaactt ctgaaccaga
     9541 cgacagcaag ggtgcgttac cttatgatac ttacatcatt gaggaattac gctctgacag
     9601 caacaaaggc tttgaactta tcccaccttt tgaaatcgtg gtatcaagaa ataaccttgt
     9661 gattgattta gggacgctga ctgatgaata cgaaaaagaa atctctatcc ataccacagc
     9721 aaccagcaaa gacggcgaaa agactatcct tgcaggaaaa gaggttacga ttgttgatac
     9781 tgtgaagctg gacggcttgg aaaaaggtac aaaatatcag ctcaaaggct ggcagatgtt
     9841 aaaggaagaa aacgctgaac ttatcattga cgggaaacgt gtagaaaacg attatacctt
     9901 tgtcgctgat gaggaagaaa tgaaagtaga aatttcctat acattcaatg cgtctgcttt
     9961 aggtggcaag aaccttgtta cctttgaaga attgtatgat ttaagcaatc cagacgaacc
    10021 cgtaaaagtt gcggaacaca aagacattga ggacgacggg caaacggtac ttatcacaga
    10081 gcgtatcatc aaaattcata cgactgctac cgataaggac ggcaacaaag agcttgaagc
    10141 tggaaaagac gttacaatca ttgataccgt aaccttagag ggattagaag tcggtacaca
    10201 gtacaaactt gtgggctggc agatgttgaa agaagaaaat atagagcttc ttatcaatgg
    10261 aaaacgtgtg gaaagtgatt atacctttac cgcagatagc gaaactatga aagtggaagt
    10321 tgtctttacc tttgacgcta cttctcttga cggcaaacag cttgtaactt ttgaggaatt
    10381 gtacgattta agcaatccag acgaaccgaa gaaagttacc gagcataagg atattgagga
    10441 taagggacag acgattacct ttaaggaaaa gccagaagaa ccagaaaaac ccgaaacacc
    10501 accgacacca gaaaagccta gcagaccgag cgacagtccc aaaacgggcg acagtacaaa
    10561 tgtaatggca tttgttgtga tgttgcttgc gtctgctggt ggactggcag gtatcttctt
    10621 ctacaaacgc cgtaaaatga agaactcata aggcggtaac ggtatgagga aagaaaaatc
    10681 acgctctccg ccgaagcggt caaacggcag acttccgctt attctggctt gtctgccacc
    10741 aacctgcaag aacacggata gtcaagggtg cgtaagcatt gattttaccc tttactatct
    10801 gggggattgc tggtacttcc cgaaccgcca gaataagaaa tcacaatcaa tttatcaaaa
    10861 atagaaagaa acgaggtaaa acaatatgga aatgaaatat gtcgtgccag atatggcaca
    10921 gtcttttgga actcttgaat ttgcaggcga aagtgagcca gtctttgaaa gagataaaaa
    10981 taaccgcagg gtcttagcca gacgaagcta taacctttat tctgacgtac agaaaggcga
    11041 aaatgttgtg gtagaaattc ccgtgcaggc tggcgaaaag aagttcaagt atgagcagaa
    11101 agtaaaactt gtcaatccga agttatacgg cagaggttac gcaatcgggg atatgggaca
    11161 taccgattat gtgttagttg ctgatgatat tgtagcagtt gaagaaaagt aaaggagtga
    11221 agaatttatg agattatcaa acggttttgt tattgacaga gagaaaacat ttggagaatt
    11281 aaaatttaca gcggtgcgtg atgtgttctt gcagaatgag gacgggacac cgagtaccca
    11341 gcttaaaaag cgtatctatg atttgaagtg cagtctgcat ggtggaatta tccccgtgtc
    11401 cgtaccgcca gaagtaccgt taagggaatt tccgtacaat gcagtcgtgg agcttgtcaa
    11461 tccagtagcc gatacggtat cacgaaaaac ctatacgggt gcagatgtgg actggtatgt
    11521 aaaggcagag gatattgtat tgaagaataa aggcaaccag aacgcaggca gtccacagaa
    11581 caacaattcg cagggacagc agaaaaaata ggcttgctga atagattttc attgtagcgg
    11641 atatgtgata cctgctataa taagtggtat atcaaaatgt aggagtgaaa caaaatgaaa
    11701 tatatttttc tacatggact ggggcagact tctttcagtt ggaataaaac attagaagta
    11761 atgagtgata agtgggatat attgtgtcct aatcttgcag actggctata taatgaacgc
    11821 ccctgctatg aaactttata taaagcgttg gaaaggtgct gtaaacagtt caacgaaccg
    11881 ctaacgctgt gtgggttatc tctaggtggt attcttgcta tgcaatacag tattgaacat
    11941 tcagaaaagg tacattcttt agtgctgata ggaacacagt atgcaatgcc aaaaaaatta
    12001 ctaaaaatgc aaaatatcat gtttcaattt atgcctagct caatgttttc aaaaatgggg
    12061 tttcaaaaag cagattttat cagcttatgc aagtctatga ttgatttaga ttttacccat
    12121 aatttgaaag atatttcttg tcgtgtattg gttatatgtg gggaaaaaga taaaccgaat
    12181 aaatcagcgt ccctacaatt aggaaattta ataccatacg caaaaattac gattatatct
    12241 ggtgcaggac atgaagtaaa tatagacgct cctattaaat tgggaaatga attaaatgtt
    12301 tttttcaata gttctcataa taccgaaagg taaaaagcaa agtaatctaa aagctgtaaa
    12361 tgcctataaa tacgctgttg caatggttcg ttgcgttaat tccagctaat gttggtagac
    12421 ataggacatt ttatttttta tgattttatg caaataacga taaaaggaga atgtctatga
    12481 cacttggaga gaaaatacaa aaattaagaa aacaaaatgg gttttcacaa gaagaacttg
    12541 cagagaaagt aacggttact cgacaaacta tttcaaaatg ggagctaaat caatcagaac
    12601 cagatttaga ctttatagct caacttagta acattttcaa tgtatctgcc gattatctta
    12661 tcaaggaaga attaacgaag cctgatgaat taccattcag aaaaaagagg tatcaatatt
    12721 acttctctga aagaagcaaa cgcacaatgt ttgttgcaat atcaatagtt gctttaatag
    12781 caagcatagt ttgtcttatt tgtgattatt ttacttctga taagctatct tggtctttta
    12841 tagctattga agcaattatt gcagtttggc ttgtattcct gccttgtatg cttttcaagg
    12901 aaaaagtaat ttttaagaca ttgataatat gcagtattat tcctatccca tttttagcaa
    12961 tacttgcctt gttattaaag gtaacgacca tttttacact tggcagttgt atttcagtcc
    13021 tttgtattgc gattatgtgg gctatttatg gaatttttcg caagtatcat caacgcatat
    13081 ttcttagttt agggttttct ctgttacttt tgatatttgt tccgattgtc attgttcgtg
    13141 ttacagacta tttcttaccg caatatgaaa ttgtttcaaa atctgatgta tttaatggca
    13201 taatcacatt tgctattgcc cttatatgtt ttggtataga ttacttaaca caacataaaa
    13261 ataagtagat attatttcaa agcagttagt cttaggattg actgcttttt tcgtacccag
    13321 aaaggagtga ttgatttatg cgtaagattt ttaacaaagg acaccgtatc agagccagcg
    13381 acaagcacct tgtctaccac tactgcatag ggacgcttct gtttgtattc atggcggttc
    13441 tcctgctatt gaatagcaaa cagctcatgc gtaccaactg ggagcatttc agcttgttac
    13501 aaaatggctt gatactttcc ccttacaatt tcataacgat actgatagcg actggtgttt
    13561 gtgtattggt cgcttttctg tattaccgct tctgttacga cagtttcaag aagctcctgc
    13621 accgtcaaaa gctggcacga atgatactgg aaaataagtg gtatgaagcc gataccgtac
    13681 aagacagcgg tttttttact gacctgcaaa gcagatcaag agaaaaaatc gtctggtttc
    13741 caaagattta ttatcaaatg gaaaagggac tgcttcatat ctgctgtgaa attacgctgg
    13801 gaaaatatca agaccagctt ttacggttag aggataaatt tgaaagtggc ttgtattgtg
    13861 agttgaccga caagaccttg catgacggtt atatcgaata taccctgctt tatgatatga
    13921 tagcgaaccg cattaccatt gatgaagtac gggcagagaa tggctgtctt agactaatga
    13981 aaaatcttgt ctgggaatat gacgcactcc ctcacgccct tatcgctggt gggactggtg
    14041 gcggtaaaac ctattttctg ctgacgctca ttgaagcctt actgcacaca aacgctgtcc
    14101 tttatatctt agacccgaag aatagcgacc ttgccgactt gggaacggtt atgggaaatg
    14161 tgtatcatac caaagaagaa atgattgatt gcgttaatgc tttttatgag ggcatggtac
    14221 agcgaagtga ggaaatgaag cgacacccga actataagac gggcgaaaac tacgcctatc
    14281 tgggactacc cccctgcttt cttatctttg atgaatatgt ggcgtttttt gaaatgcttg
    14341 ggacgaaaga aagcgtgggt ttacttagcc agttaaagaa aatcgttatg ttagggcgac
    14401 aagcaggtta tttccttatc gttgcctgcc agcgtccaga cgcaaagtat ttctcggacg
    14461 gtatcagaga taacttcaat ttccgtgtgg gacttgggcg tatcagcgaa ttaggttacg
    14521 gtatgctgtt tggttcagat gtgaaaaagc agtttttcca gaagcgtatc aaggggcgtg
    14581 gctattgtga tgtgggaaca agcgttataa gcgaatttta cacgccttta gtcccaaaag
    14641 gacatgattt tttacaaaca atcggcaggc ttgcacaaga aagacaagtt atgccagaac
    14701 aacaaggaaa ggagaactaa ctcatggaaa taaaagaaat caataaaata atacaatcag
    14761 aagataaaga cgagaaagtt aagagcgtaa aatgtatctg tatcaattat ggagattttc
    14821 tagccgattg cgagggattt gtgcagaaac gttatcacga ttttaaatgt aatccaaaac
    14881 atcagtttga gaaaaaagct gatactattc ttgaaaatgc aattcacgaa aaaaacttca
    14941 tgcccgattt gttcttgata cgcttaaata gaaaacagtc ggcttgcaac agccagattg
    15001 attttgtttt tgaattgctg gataagtctt ttctggaaac agaccctatc cgtaaaagtg
    15061 aaatatcaga agaaacatta aatttatgtt tatccgctga tgtttcattc atcatggttt
    15121 acattggtat gaacaggtag gataggttct gcctgcacaa cggcaggaac ggtcggcgga
    15181 gcaaatacga ccgtggaagc cgttttgcag agcagggctt gtccctgctc tgctgatgaa
    15241 cccctcggta tctaacagag gggtacgttt gcaaatagac aatagacaaa aaacatagga
    15301 aacataaggc ttctgacatg gtttcctagc aaaaatcggc tgtgaagtcg ccttaaaaaa
    15361 cttcacacgt tacagattgg gggtatggtt ctgaatgaag aacaatggat aaaagaatta
    15421 cgggagaaac ggattgctta cggtatctca caaggcaggt tggcgttagc ttctggcatt
    15481 acaagagaat acctcaacaa gatagaaagc ggaaaaatga agccgtcaaa ggaacttctt
    15541 aatattctgc atgaggaact ggcaaggttc aatccagaag caccgcttac catgctgttt
    15601 gattatgtga aaattcgttt ttccacgctg gatatacagc acatcatcaa agatatatta
    15661 aaactgaata tcaattatat gctccatgaa gattacggac attacagtta tacggagcat
    15721 tactctttag gggacatctt tatctatacg tcgtctgacg aagaaaaagg tgtcctttta
    15781 gagttaaagg gtcggggttg ccgacagttt gaaagttacc tgctggcaca gcaaagaagc
    15841 tggtatgact tcctcatgga cgcactcata gacggtggcg tgatgaagcg tatcgacctt
    15901 gctatcaacg accatacggg cattttggat attccagagc ttgcggaaaa atgcaggaaa
    15961 cgggaatata tcgggaagtc cagaagttat aagttctacc agtcgggcga gcttatcaag
    16021 cacagagagg acgacagaga atatatgggg cgtacccttt atcttggttc gctgaaatct
    16081 gatgtatatt tctgtatcta tgaaaaggat tatgagcagt acgtcaagct ggggacacct
    16141 ctggaagaag ccgacattat caaccgtttc gagatacggc ttcgcaatga acgtgcctat
    16201 tatgcagtac gagatttgct gacctattat gacgcagagc agaccgcctt ttctattatc
    16261 aatcagtatg tgcggtttgt tgatgaagaa ccagacaagc gaaaaaatga ctggaaactc
    16321 aatgaccgct gggcttggtt tatcggcgat aacagacaga gtttgaagct gacgacaaag
    16381 ccagagcctt acaccttaga ccgcacattg cgttgggtac aaaggcaggt agcaccgacc
    16441 ttgaaaatgc tgaaaaagat tgataaagga aacggtacag actacatgga tacaatcgaa
    16501 cagcaggcaa agctcacaga aaagcatgaa atgataatca aacagcagac gacccctgca
    16561 aaagatttag tagaaagtta ggagtgatga aaaatgtggg tattattgaa agatttcctg
    16621 ctggtatcta tgggaatggg tattggcgta gtcttgatgt gtattttgaa tgttggcaaa
    16681 gaagctgact ataaaatgaa acaattagaa gaaagcgagg acaattaaat gaatttcgga
    16741 caaaatttat atcaatggtt tttatcaaac gcacagagct tagtgcttat ggcgattgtg
    16801 gttatcggta tctacttagg tttcaagcgt gagttttcca aacttatcgg cttcttggta
    16861 gttgccttga ttgctgtcgg cttggtattc aatgctggcg gtgtgaaaga tgtactgtta
    16921 gagctgttca ataagattat cggtgcataa aattttattg tggtgctgat atgcgaatgg
    16981 tataatgtgg atatgaaaca cagtatctag gagtgatgaa atgaaatata taatgcttca
    17041 tggtttggga caatcttctg aaagttggag tgataccgta aaagtattca atgatgattt
    17101 tgaagtttta tgccctaatt tatcagattg gctttctggg aaaacaccat gttatgatac
    17161 attgtatcgg acattggaaa catactgtga acagtttgaa gaaccgctaa atatttgtgg
    17221 actttcttta ggtggtattc ttgctatgca gtatgcgata gaacacccag aaaaagtgaa
    17281 ctcattagtg ttaataggaa cacaatatga aatgccaaag tatctgttaa aaatgcaaaa
    17341 tctgatattt cgtattatgc cttgttcagc gtttgaaaaa atgggctttc ggaaaacaga
    17401 ttttatcaat ctatgcaaat ctatgatgga acttaatttt aaacatgaac tgaaaaaaat
    17461 atcatgttcg gtacttgttg tctgtggcga taaggataag ataaataaaa aagcttcttt
    17521 agaattgcaa caacagatag aaaacgcaga aatagctatt attgaaaatg caggacatga
    17581 agtaaataca gataatccaa cactattagg gcaaaagtta aatgtatttt tcaaacaata
    17641 tgtctgataa acaatttcat atccctacac acaaagcagt tagtcttagg attgactgct
    17701 tttttcatac ttaaatttca gattggagtg attacatgaa cgatattttt aaggatatgc
    17761 agtcaaaagt cggctgtgat tatatttctg acctgccctc ttacaagcgt aaggtatggc
    17821 atgaaatgaa acgactgaac cttgctgact atgaagaaag acagttagaa gatttttcta
    17881 aatatgtgtt tggtatgtcg taccagacct taaaagatgt gatgaaacaa cagaaaggac
    17941 gtgaggaaca atgcaggaaa caagggtgct ggtggaaacg agaggaacaa ctggcgaaga
    18001 aacaatatca tactggtttg aactgccgat agatgttgcc gagtttgaag aaaagctagg
    18061 tgtcggtgca gaaagtgggg attaccgtat tattgaaaag gtgttgcctt atgctgatga
    18121 agtccacgaa catacaagcg tgtatcagct caatgaatta gattttatgt accgccagct
    18181 ttcaagtgat atgcaggaag aatatgtatc actacttacg gtgtttgaaa atttagaagc
    18241 actttatatt tgcaggaacg tgattaccgt ttatccagac tgcaaaagca tgatagatgt
    18301 tgcaaggcaa aagctgatga acgacccgac atttaaacat ctgtccgagg actgtcaaga
    18361 atactatttc gattttgaag cctacgcttc tcacttacag gaacacggga aatttttagt
    18421 aacggaacac ggtatctttg aactgccaga gtaggaaatg aggtggtgct tatgattgat
    18481 gatatggcgg tttatattgc taatcttggc aaatacaatg agggctattt agtcggtgct
    18541 tggttcacgt ttcccattga cgaggaagat gtaaaagaaa aaatcggctt gaatgaacag
    18601 tatgaggaat acgctatcca tgataccgac aacttcccca ttgcgattgg cgagtatgtt
    18661 tccattgaag aactcaacga gatgtatgaa atgatagagg aacttcccga ctatatcgta
    18721 gagtgtctgg acgaatttat cagccacttt gggacgctgg aagaagtcgt ggaacacaag
    18781 gacgatattt attattatcc cgactgtgaa accatgacag atgttgccta ctactacata
    18841 gacgaattgc aggcactagg ggatattcca cccagcttgc agaactacat tgactatgaa
    18901 gcctacgggc gagatttgga tatgggcggt tgcttcattg aaacaagccg aggtatgtgc
    18961 gagataccat actaacgctg gaagatcagc gacaagcatt gttgctactg acagcgtatt
    19021 tctctttaag ggacagtctg aaaatggctg tccttttcca tttagaaact tacgaaagga
    19081 gctgaaagaa cttgaaaaag attaaatctt atacgggtat ctggaacgtg gaaaaagtct
    19141 tgtatgcaat caatgacttt aacttaccct ttcccgttac ctttacacag attacatggt
    19201 ttgtgattac ggaatttatc atcattctgt ttggggatat tcctccactt tccatgattg
    19261 agggagcatt tctcaaatac tttggtattc ccgttgctct cacttggttt atgtcgcaaa
    19321 agacctttga cggaaagaag ccgtacagct ttttgaaatc acagataact tatgcgttgc
    19381 gacctaaaat cacttatgca ggaaaagccg taaaactgca taagcagacc ttgaatgaaa
    19441 caatcacggc agtaaggagt gtgaactatg ttcccgataa aatatattga caataacctt
    19501 gtctggaaca aggacaatga ggtgttcgct tactatgagt tgataccgta taactattct
    19561 ttcctatccg cagagcagaa atttatcgtg catgacagct ttcgccagct tatcgcacag
    19621 tcccgtgagg gtaaaattca tgccttgcag attgccacag aaagctccat acgaagtatg
    19681 caggaacagt caaagaaact ggtaactgga aaattaaagg aagttgccta tcagaagata
    19741 gacgaacaga ccgaagcgtt agtatctatg attggggaca atcaagtgga ctaccgtttt
    19801 tttcttggct ttaagctcat ggttacggaa gaacagctca atctgaagaa catcaaaaaa
    19861 tcggcgtggc tgacgtttac ggaatttctc catgaagtga accacacgct gatgaatgac
    19921 tttgtttcca tgccgaatga tgaaatcaac cgttacatga aaatggaaaa gttactggaa
    19981 aataaaatat caaggcgttt taaggtgcgt cgcttggaaa tcaatgattt tgggtatctc
    20041 atggaacatc tttacggcag ggacggtatc gcctatgaag attatgagta ccagctacca
    20101 aagaaaaaat tgaacaaaga aacgctgata aaatactacg accttatccg tccgacaagg
    20161 tgtgtgattg aggaaagcca gcggtattta cgattggaac atgaggacaa ggaaagttat
    20221 gtgtcctact ttactgtcaa tgcgattgtc ggggagcttg attttccgtc gtctgaaatc
    20281 ttctatttcc agcaacagca attcacattc cccgttgata cttctatgaa tgtagaaatc
    20341 gtggagaacc gaaaagcatt aacgacagta agaaacaaga aaaaggaatt gaaagacctt
    20401 gacaatcacg cttatcaagc aggaagtgaa accagctcaa atgtggtgga cgccttagac
    20461 agcgtggacg agctggaaac agacttagac cagagcaaag aaagtatgta taagttaagc
    20521 tacgtgatac gggtgtctgc acccgatctt gacgagctga aacgccgttg tgatgaagtc
    20581 aaggactttt acgacgacct caatgtaaag ttggtgcgtc ctgctgggga tatgctgggg
    20641 cttcattctg aatttcttcc agccagcaag cgatatatca atgactatgt gcagtatgta
    20701 aaatcagatt tcttggcagg gcttggtttt ggtgctacac agcagttagg agaaactacg
    20761 ggtatctata tgggctattc cgttgatacg ggaagaaatg tgtacctgca accgtcttta
    20821 gcttcgcagg gcgtaaaagg cacagttaca aatgcccttg cttctgcttt tgtcggttcg
    20881 cttggcggtg ggaagtcgtt ctgcaacaat cttctggtgt attatgctgt gctgtttggc
    20941 ggtcaagcgg ttatcttaga ccctaaatca gagcgtggca actggaaaga aacgctccca
    21001 gagatagccc gtgaaatcaa tatcgtaaac cttaccagcg acaaggacaa tgcaggactt
    21061 cttgacccat ttgtgattat gaaagataaa gaggacggag cgacgctggc aaaagaaatc
    21121 ttaacatttt taacgggtat ctctacccgt gacggagata aattccctgt gcttattagt
    21181 gcaatcagca aggtatctga aagcgaacac agaggactgt taaatgttat cacagaatta
    21241 cggcaagaga atacaccgat tgcgaaccat atcgcaaatc atattgccag ttttacaaat
    21301 tatgattttg cacatctgct gttttcggac gggacagtag aaaatacaat cagtctggat
    21361 aaccagctca atatcattca agtagctgac cttgtactgc cagataaaga taccactttt
    21421 aacgaataca cgaccattga attattatcg gtggcaatgc tgattgtgat tagtaccttt
    21481 gcccttgatt ttatccattc agacagaagt atttttaaga ttgtagacct tgacgaagcg
    21541 tgggcgttct taaatgtggc acaaggcgaa accttatcaa ataagctggt tcgtgctgga
    21601 cgagctatgc aggcaggcgt ttattttgtt acacaatctt ctggcgacgt gtcaaaggaa
    21661 agtctgaaaa acaatatcgg tttaaaattc gctttccgtt ctactgacat caacgagata
    21721 aagcagacct tagagttttt cggtatcgac aaggacgacg agaacaacca gaaacgcttg
    21781 cgtgatttgg agaacggaca atgcttattg caggacttat acgggcgtgt cggtgtggta
    21841 cagatacacc ccgtttttga agaactgcta cacgcctttg atacaagacc gcccgtacag
    21901 agaaatgagg tggagtgatg aaagaaagga taaaaggtgc gttcacaaaa aagaagattt
    21961 tccactttct caaaatggct ctgttcgtag tggcactctc ccttatcctg ctttcacttc
    22021 tggggacggt agctcatgcg acggggcttg tggacgatac cataaacgta gaaaatcttt
    22081 actcaaaata ccccctttcc aactaccagc ttgattttta tgtagataat agctggtcgt
    22141 ggcttccgtg gaactggcta gacgggattg gaaaatcggt gcagtacggg ctttactgta
    22201 ttaccaactt tgtctggacg ataagccttt atttgagcaa tgccacgggc tatgtggtgc
    22261 aggaagccta taaacttgat tttattaacg atatggcaga cagtatcgga aagagcatac
    22321 agacccttgc aggcgtaaca cagaacggtt tttccagctc tggcttctat gttggtttcc
    22381 tgcttctcat tatcttggtg gtgggacttt atgttgccta tacgggactg ataaaaagag
    22441 aaaccagcaa ggcacttcac gcagttatca actttgtggt ggtgttcgtg ctgtccgctt
    22501 cgtttattgc ctacgctccc gactacatca aaaagataaa tgaattttca tcagatatta
    22561 gtaccgcttc acttgatttg ggaacaaaaa tcatgctccc aaactctgac agcgagggca
    22621 aggacagcgt ggacttgata cgggacagct tattttctat tcaagtggaa cagccgtggc
    22681 tactcttaca attcgggaac agcaacgcag aggaaatcgg gacaaaccgt gtagaagctc
    22741 ttgtatcggc aagtccagag gtcgaggacg gaaagaccag agaggaagtc gtgaaaacag
    22801 aaatcgagga caacgacaac aacaatctga cgataccgca ggtggtaaac cgcttgggta
    22861 tggtgttctt cctcctgttc ttcaacttag ggataacgat atttgtattc ttgcttacgg
    22921 gcatgatgtt gttcagccag atacttttta ttatcttcgc tatgtttctg cctatcagct
    22981 ttttactttc catgataccg agctatgaaa gcatggcaaa gcaggcaatc gtaagggtgt
    23041 ttaataccat aatgacacgg gcaggaataa cgctcattgt aacggtggcg ttcagcattt
    23101 ccagtatgtt ttataatata tccacggact atccgttctt tatggtagcg ttcttacaga
    23161 tagtgtgttt cgcaggtatc tacatgaagc tgggcgattt aatgagtatg ttctctttga
    23221 acgctaacga cagtcaaagc atgggacgaa gaattttccg cagaccatat ctatacttgg
    23281 cacatagggc taggcgtatg gaaagacggc ttgctggtgc ggttactgct ggcggtgttg
    23341 ctggtgcggt ggcaggtagc acgatttcaa acagaaaacc gacaaggaac acagctacaa
    23401 aagacagtcg gggcaatacc tctacttcca gcatgggaca gcgtgcaggc tcaaaggtgg
    23461 gtgctgtctt agatacgaaa aataaagtga aagacaaggc aaatgctgtc aaagaaaaca
    23521 tcaaggatat gccaacacag accgcttatg cggtgtattc cgcaaaggaa aaggcaaagt
    23581 ccagcgtgtc cgactttaag cgtggcatgg tacaggaaca gcagtccaga cagtcggggc
    23641 gtttggaaaa gcaggaacag cataagaaaa atatcgctga caagcgtatg gagcttcaaa
    23701 aggcacaaga agcaaggcag gcacagcgaa aggctgacgg atcagcgaca acgggagcta
    23761 cccgtcccca tgagcgacca gccacagctt caaagccgag tgcggaaaaa atgcaggaag
    23821 tcaaacgccc tgccatagct caaactccga aagcaagtga gccagtcaag acaaatgtta
    23881 tcaaagagcg tccgttatct tctggtgctt ctgataagaa agcgacccag tcggcacaga
    23941 taacacatag gcaaaatgta gaaaaagtgg tatcgcagga aacacgccag aattacacca
    24001 aagaccgtag gacaaaggtt cagcagacca cagagaaaac ccgtaacctt gtaacgaaga
    24061 aaggacagaa gaaaaaatga aactgaaaca tatcgctctc attggcagtc tgtttcctat
    24121 cctcttttct ctggtgcttt tctttggggt gttgattagt gcggacagcg acgacgagaa
    24181 cagcaatttt tcttctggca ttacgggtat gaacttatcc gcagaagtct tgaaacatca
    24241 gcctatggta gaaaaatacg ccagagaaaa cggtatctcc gagtatgtca atgtattgct
    24301 tgctatcatt caagtagaaa gtggcggtac ggcagaagat gttatgcaga gttcggaaag
    24361 tcttggttta ccgcctaatt ccttagatac agaaagttca attaagcagg ggtgtaaata
    24421 ttttgcgtcc ctgctttctt cctgcaaaaa tcaaggtatc gacgatttga atgtagcgat
    24481 acagtcttat aactatggcg gtggctatgt gggatatgtg gcaggaaaag agaaaaaaca
    24541 cacctttaac cttgcagaga gcttcgcccg tgaaaaatcg ggtggaaaga aagtaaccta
    24601 caccaacccg atagccgttg cgaagaacgg gggctggcgg tatggctatg gaaatatgtt
    24661 ctatgtggaa ttagtcaatc aatatttgac cgtggcacac tttgataatg cgacggcaca
    24721 agcgattatg aatgaagcgt tgaaatatca aggctggaag tatgtgtatg gtggcagtaa
    24781 ccccaacact tcctttgatt gtagcggact tgtgcaatgg tgctatggaa aagctggtat
    24841 ctccttgccg agaaccgcac aagcacaata tgacgctacc caacatcttc cgctttcgca
    24901 ggcaaaggct ggggacttgg tgtttttcca ttctacctat aacgctggtt cgtatgtaac
    24961 ccacgtcggc at
//
LOCUS       DACULQ010000042        24798 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.042, whole genome shotgun sequence.
ACCESSION   DACULQ010000042 DACULQ010000000
VERSION     DACULQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.042"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            243..1064
                     /locus_tag="JA154_06815"
     CDS             243..1064
                     /locus_tag="JA154_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAT7995576.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            complement(1201..1350)
                     /locus_tag="JA154_06820"
     CDS             complement(1201..1350)
                     /locus_tag="JA154_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995577.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSNDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(1357..1842)
                     /locus_tag="JA154_06825"
     CDS             complement(1357..1842)
                     /locus_tag="JA154_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995578.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            complement(1855..2607)
                     /locus_tag="JA154_06830"
     CDS             complement(1855..2607)
                     /locus_tag="JA154_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7995579.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            complement(2607..2744)
                     /locus_tag="JA154_06835"
     CDS             complement(2607..2744)
                     /locus_tag="JA154_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT7995580.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            complement(2784..3413)
                     /locus_tag="JA154_06840"
     CDS             complement(2784..3413)
                     /locus_tag="JA154_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7995581.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            complement(3374..3937)
                     /locus_tag="JA154_06845"
     CDS             complement(3374..3937)
                     /locus_tag="JA154_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995582.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            complement(3947..4171)
                     /locus_tag="JA154_06850"
     CDS             complement(3947..4171)
                     /locus_tag="JA154_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995583.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            complement(4176..5267)
                     /locus_tag="JA154_06855"
     CDS             complement(4176..5267)
                     /locus_tag="JA154_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7995584.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            complement(5302..5760)
                     /locus_tag="JA154_06860"
     CDS             complement(5302..5760)
                     /locus_tag="JA154_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995585.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            complement(5753..6838)
                     /locus_tag="JA154_06865"
     CDS             complement(5753..6838)
                     /locus_tag="JA154_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAT7995586.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            complement(6865..7065)
                     /locus_tag="JA154_06870"
     CDS             complement(6865..7065)
                     /locus_tag="JA154_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995587.1"
                     /translation="MFIKKMSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQK
                     KKYDRLNKDTIERAIVEAINNN"
     gene            complement(7212..7544)
                     /locus_tag="JA154_06875"
     CDS             complement(7212..7544)
                     /locus_tag="JA154_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAT7995588.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            complement(7800..8612)
                     /locus_tag="JA154_06880"
     CDS             complement(7800..8612)
                     /locus_tag="JA154_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HAT7995589.1"
                     /translation="MIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQDGIIAGLGV
                     FKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYLQRMSGIAT
                     ITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGILLKDNHIG
                     AAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSLEMAKQAVD
                     IINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLRNI"
     gene            complement(8617..9918)
                     /locus_tag="JA154_06885"
     CDS             complement(8617..9918)
                     /locus_tag="JA154_06885"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088883.1"
                     /note="catalyzes the formation of oxaloacetate from
                     L-aspartate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAT7995590.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            complement(9931..10845)
                     /locus_tag="JA154_06890"
     CDS             complement(9931..10845)
                     /locus_tag="JA154_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAT7995591.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            11263..12720
                     /locus_tag="JA154_06895"
     CDS             11263..12720
                     /locus_tag="JA154_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HAT7995592.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            12943..13371
                     /locus_tag="JA154_06900"
     CDS             12943..13371
                     /locus_tag="JA154_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995593.1"
                     /translation="MNNKLHELEKNLPEISCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            13431..13739
                     /locus_tag="JA154_06905"
     CDS             13431..13739
                     /locus_tag="JA154_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HAT7995594.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(13886..14908)
                     /locus_tag="JA154_06910"
     CDS             complement(13886..14908)
                     /locus_tag="JA154_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAT7995595.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(15083..15589)
                     /locus_tag="JA154_06915"
     CDS             complement(15083..15589)
                     /locus_tag="JA154_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAT7995596.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            complement(15649..16353)
                     /locus_tag="JA154_06920"
     CDS             complement(15649..16353)
                     /locus_tag="JA154_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995597.1"
                     /translation="MMGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGL
                     SIKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHAS
                     NTYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEIL
                     ALVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKS
                     NEHKCKSSIVIKNEQN"
     gene            complement(16568..17638)
                     /locus_tag="JA154_06925"
     CDS             complement(16568..17638)
                     /locus_tag="JA154_06925"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088891.1"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HAT7995598.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            complement(17691..18266)
                     /locus_tag="JA154_06930"
     CDS             complement(17691..18266)
                     /locus_tag="JA154_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HAT7995599.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            complement(18268..20055)
                     /locus_tag="JA154_06935"
     CDS             complement(18268..20055)
                     /locus_tag="JA154_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HAT7995600.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            complement(20116..21375)
                     /locus_tag="JA154_06940"
     CDS             complement(20116..21375)
                     /locus_tag="JA154_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase"
                     /protein_id="HAT7995601.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            complement(21637..23628)
                     /locus_tag="JA154_06945"
     CDS             complement(21637..23628)
                     /locus_tag="JA154_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAT7995602.1"
                     /translation="MRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLMENQ
                     GRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVVLIL
                     GDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEIVIVGGGLACSFAR
                     QYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAIDSNG
                     SIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINNLIV
                     NTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKDKLT
                     KEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAENLL
                     ESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEERQIM
                     RIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRREDIIP
                     LFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYVVTG
                     KIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKEVNK
                     YVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            complement(23645..>24798)
                     /locus_tag="JA154_06950"
     CDS             complement(23645..>24798)
                     /locus_tag="JA154_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT7995603.1"
                     /translation="AHSNNSISVTERVSIKIDLIWAYLKEDMNKEAEVILLKTIKLMK
                     VVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYKSFGDVFIYNSKLD
                     YIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYGLIANGYELKKDFK
                     LAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLVDDNKKLENDILKD
                     GLTKLYNRRYLEQQLDLFNETRDKNMVAILVDIDCFKSYNDNYGHLAGDKVIVKVTDI
                     IRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRVFKLLEFEGIEHIY
                     SKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQYIII"
BASE COUNT         8024 a         4467 c         2677 g         9630 t
ORIGIN      
        1 caaacgatac cattatttgt aattaagaat tttggaatta ttgattacaa agtttttaca
       61 ttctcatctc acataagtaa tatttttatc aaaaaattaa tattattaaa ctttctaaat
      121 tacttatctc ttgatataat agtatacaaa acaaaccata tttcccactg aaactttgtt
      181 caacactagc tgtgtcaacg ttttcacagg aaatttgagt atataattta ttggaggttg
      241 atatgtacaa attaatcgca ctagacattg atggaacaat attaaataca caaaaaagga
      301 ttactcctga agtctttgag tccattcagg aggcaaaaag ggctggggca aaagtagtta
      361 taacaacagg cagacctctt cctggtgtaa aagaacttct aaatcaacta aatttaacag
      421 atgaaggtga ttatgttata tgctttaatg gagcaattat tcaagaagtt aaaagtgaga
      481 aaatcataca tgatgtagaa atgagtttag atgattttga ttttatatat aataacgttt
      541 gtaaaaaata taagactaaa atacacataa atacaatgac aaatttaatt acaccaaatg
      601 aaacgcctgg caaatataca cttcatgaag cgaaattaaa taatatagaa gtcaaatata
      661 tacaaaaaga taaaattgat gaatctatta aaatctgtaa aattatgata gttgatgaac
      721 cagaaagatt ggaagaaatc atacagcaac tacctaaaaa tttattcaat aaatatacta
      781 tagtaaggtc tgctcctttt tatctagaat ttttaggtaa aactactaat aaaggaactg
      841 ctcttaaaac attatgtacg aatctaaata tacctataga aaatgctatt gctgttggcg
      901 atgaagaaaa tgaccaacat atgataaagt atgcaggtct tggtgtggct atggggaatg
      961 cacgtaatag tataaaggaa attgcagatt atgttactga tactaataat gaaaatggag
     1021 ttgcaaaggt tataaataaa tatatactta ataaggctat ctaatcatct aaaaaaaact
     1081 aaaaatatac ctttagattt aaatactttt cacatcccta ctctatacta atttatatag
     1141 tatggatatt acctatcatt tttatctaaa ggtcattttt agtttttcat atatgtatat
     1201 ctatgatagt accttttgtt tgagtttaaa tattttatat gattctacta ttgcctcatt
     1261 aatatctaaa caatatatat cttcatcatt agataatccc gcttcgtttg tccaagcatc
     1321 aaaatcactc ttcgtccaga agaaattcat tatatttcag caactagcag cccaatttag
     1381 atgcttatca agatttatat gaagcactct taagtttgga ttattcacat actcaatctt
     1441 ttcatttttt ataattactt ctatttcctt acctgtagca ctacaaatag agtttatttt
     1501 tatatcttgg ttgaatgtgc aagaggttcc tatagcatct atagcacaca ttgcactgaa
     1561 acttctatta tctctcaaag ttactttatg atttgttggc aatgctgata caggatatat
     1621 aaaatttata ctattcccat caacaaccat tatattttta taaataaaat attgtagtgt
     1681 cctttctata tattcttttt ctatattcaa ctttttactt atatattcag tcacttcttg
     1741 aagtgtaaca ctagaacctt tatctattat catatccata atagagattc ttattttctg
     1801 ttgtgtatta tccaaactat tgtatttttc tatttgactc atgtctactc ctctttaaac
     1861 ttcataacac tttgataaag tatcagttaa tttgtttttg tactcttcat atttttggct
     1921 tcctctaatt ctaggtcttg gaatatctat atttatatca tcaataattc ttcctttatc
     1981 tctcgacatt actataactc tatcactcat atatacagct tcatcaacat catgagttac
     2041 cattaatgca gttattttct tctttatcca tattcgttct agctcttcct gtaaattttg
     2101 tctcatttga aaatctacag ctcctaatgg ttcatccatt agtaatacct ctggattaca
     2161 tgctaaagct cttatcatgg ccactctttg tttcattcct ccagataatt gatgcggaaa
     2221 cagtttttct tttcctttca agtctgacat aaccaaaagt tcttctagca tttcttctct
     2281 tttttctttt ggtattttct gctgtttcat aggaaataga acattacctc ttatagtttt
     2341 ccaaggaaat aaagcataat tttgaaatat aaaagctcta tctatgcccg gttttttaac
     2401 tcttttatca tttataaaaa catctccacg ttctgatttt tgaaacccac ctattatagt
     2461 aagtaaagtt gtttttccac aacctgatgg tcctaaaaga cagacaaatt gtccatcatc
     2521 tatatccata cttatatctt ctaaaacctt tgtatgtact ctattattta caaatgtctt
     2581 atttatatct cttacactta acttcattaa tctgcaaacc tccatctaca aaggatttta
     2641 tttataaact ttaataccct atctattata aatcctatta aagctgcaaa tatcataagc
     2701 ccaattaata catctgtatg cattaatgtt tgagcttgta ccattgagta accgagaccg
     2761 gcactcgtag ctataaactc ggctcagata acagacatcc atcctgtact tatagcaatc
     2821 cttgaaccag ttagtatatc tggaagagat gcgggaacaa ttacatttgt aaatatcaca
     2881 attggcgatg ctcccatact tctagctgca ttataataat cctttgatat tgctcttact
     2941 ccttgaatag tatttaaaat tatagggaaa acccctgaaa aagctattaa aaatattgtt
     3001 ggaccatctc ctataccaaa ccatactata gtaagaggaa cccatgccat tattggaact
     3061 tgccttacag aatctactac tggagataaa accctttcaa aagttttaga aaatcccata
     3121 ataaatccta ttggaagccc aaacaataat gcatacataa aacctttaag tactctctgc
     3181 aatgttataa aaagattagt gactgtctct gcatctgtca cacagaaaaa gaaacctttt
     3241 attacatcta ctggcattgg aagtaacaaa gaactcccta tatcttttgc tgcaaaatgc
     3301 cagactaaaa atataataac taatagtatt attttatata aaatatccat actaccatca
     3361 gtttttttct tagttaatct atcgctttta tctttagaaa ttttaatttc caattgctta
     3421 tttcctccat atctatattt cttatattcc tgtactcttt taatgctttc ttaaatgtag
     3481 ctttattctt taaagcatca acacttttat tgtaaagtgt tataaaatca ctatatgctt
     3541 tacttttttt aaactcttta cttacaacta atacataggt atcataatct cctaatttag
     3601 ttgtagattt tcttttgccc tctaacccaa ttgactttat gcaatctatt acaacaccat
     3661 ctactaaatt actttctaat ccatatgcta gtgcagtccc taataaggga gctacttcca
     3721 cattcttgta gtatttttta accaattctg actggtaatc tctattttga atgactccta
     3781 tcttttcagg attattatca cttaatagaa atatatcaga attttgaact acagtaccaa
     3841 caatttcaaa tccactattt acatttacat attgttttgc agcatcttta catatgattg
     3901 ctatatctat ttcctctgaa ctcagagccc actgcattgt acttgctcag caatcattaa
     3961 ttgtaaaaat atccattata tcacttactt ttatattttc aaagtagtct ctatttacca
     4021 tataatttat tacaaaacca gaggtatcat cagatacccc tattcttaat tttcctttta
     4081 tttcttcttt atcttcaaaa ttaaaaaata ttataaatat acttcccaaa ataatgataa
     4141 aaagtattat tatatttttc atattctcca tttatttata atacctttgc tttccagtct
     4201 tcataactca taccttcagg aaactctggg ttaacttttt cttttataaa tttgtcaaaa
     4261 tcatcagctc cacaagagtc caataagctt gtatcaatcg tatcttcata tttaggtata
     4321 tcatctaaag cctttaaatc tttatacatt tgtaaattat ttttatattc ttcacctgta
     4381 actttccaag taagagttct accttctcca acagtcttct tatagatagt tctaagtgca
     4441 acttcttctt ctacattata atattttgca aatattttag ctgcttcagc tggatttgta
     4501 tatatatatt ctattgcttt tgtatgtgct agaaccaatt tttttgctaa atctggatgt
     4561 tcttttataa agtttttgtt taatgagaat gaacaacagt tatattcttt gccattagct
     4621 ttttctttat attgagtaga tgctattatt tttcctgcac cgtcattttc agctacagaa
     4681 ccccaagggt cacaagatgt aaaagcttga attttcccag ttttaagagc taaatattta
     4741 tctttgtctg aatccatatt tacaacttca tattttgatg ggtcagttgg tagacctgtt
     4801 tctggaccat aatcacttgt ccataagaaa tcactcatat ctgttgcaat ttttttacct
     4861 attaaatctt taggttcttt tatatctttt gaaactatta aatattcaga accaccagta
     4921 tggttgttag ctgctattgt tattggtgaa cccttaggta ttgctccaac taacccctta
     4981 gttcctatgt aacctgcatc catttttcct gctgccatag cttcaggaac tttaccattt
     5041 ccaacagttt ttacattaag acctaaatct ttatatattc ctgccgattc tgctactggt
     5101 cccgcagtca tatggtcaca attataataa cctactgtta ttgtataatc atcttttcca
     5161 gtttttgctt cttctttttc ttttggttta ctactacatc ctgttaatat agatgtaccc
     5221 attacaccaa ataagattaa tgcttgaatc ttcttagata atttcatata aatttcccac
     5281 ctttatgtat tatttttatt tttagcaaca tttgtccata tcttcgccaa ataaatcaac
     5341 aaatttttta ccctctactc tgtaaatagc atatcctaca ttaccatttt tagaccctga
     5401 tacaattact tctattggtg ctttatcatc aatattttta aaggttatag tttgattctc
     5461 tataggtgct ggtattggct ttgttatata gtggctattt ttatcagaaa ctacgactac
     5521 catttcattt gaattattgc tttttttata tataactact aaatcttttt tcccatcatt
     5581 atttaagtct tcttcagcac aagttattat gtcttcttta taattttttc taaaatattc
     5641 taatgtttct ttattcacat ttggaataga agcatcttct ttgatgttat catcaactat
     5701 ttttgataaa atgattaaca caactactat gcaggatatt ataactatct ttttaagcat
     5761 gatttcctat gacctctttc atctttggta ttattgattt aaatgcaaat aaaataatta
     5821 aaatagtaca tacatactca aaactatcag gtatatttaa cattagttta tttgccaact
     5881 gtattgaaga atttgttgca tctaaattta ctgagttatt attcactaaa agtaaatttg
     5941 ttatatatcc aattattatt gaagacagtg tcaaagttac agaatatata ataacagttc
     6001 tttttccaat aagtttaaac atacttataa gttctggtag atttgtagct gctccagcta
     6061 ttagaaatgt tatcgctata ccaggagatg ctccacttgc aacaagggct gctataaacg
     6121 gtatatgtcc tacagcacat acatacataa cacatgcaag tattgctata cttccaagag
     6181 atagcaaacc tggatttcct aaatattttt gaattatact atttggaaaa atagttgtta
     6241 taaatcctgc aaaaaccata cccattacta catatttact aatagtaagg gctaattcac
     6301 caaaagacca tttaagacca tcaatcatat tttccataaa acttcttttt tcttcttcta
     6361 attctattac ttcttcctct gaatcattat gaagtgataa ttcttctttt ccaaaacaat
     6421 ttcctaaaaa tcctactaaa aatggtacta aaaaaccagc tattagataa atgattgtaa
     6481 ctttaggacc taaaagacct agacaaagaa ctacagctat tggatttatt attggagttg
     6541 atgtcataaa agcaagtact ggtcctaaat atgctcctga ataatacata cttattccaa
     6601 gtggtattac tccacaacta catattggta aaaacattcc tgagatagta cttttaatta
     6661 aagaagaaaa tttcttattt ccaagttgtt tttgaaacct tcttggagtt aaaacattgt
     6721 gaagtacacc tgccaagata aaacttatca taagccatcc tgatgctcca ttcagtgtaa
     6781 ctatagaagc tactatagta tctttaactc catttaaaac aatttctaat atattcattt
     6841 ttgatactcc taatttttaa acttttaatt attgtttata gcttctacta ttgccctttc
     6901 aattgtatct ttatttaatc tatcatattt ctttttctga tttattatta aagttccttt
     6961 tgttataatc ccatattttt ttatatacga aaaatctttc cctgcttgat ataattttat
     7021 ttccaattta tcagcatact tttctgacat tttttttata aacactccat gttcatcaca
     7081 agatggtcaa gtgtttataa attcaactaa aattttagac atttgttcat cccctacttt
     7141 aaacaattta tcttttactt aaaataatcc acagaaaata aatttttcta tggattgtat
     7201 cctatactaa tttataacgt tataacttgg tccacacctg cactgcctaa acaagcatat
     7261 aggtttggga aacatccaat tgtagctcca tcaaccaaat tattctctat acctctttct
     7321 atagcacatt ggtcacaagc cattattatc atatcagttt tttcatgtaa tttacttagt
     7381 ctctcgccta caggattacc tttagataaa aataaggtat tatccataaa gaaaaacatc
     7441 cctagaacgt ctactccatg agttccttgt tcaagttgtg gtattatcat tttgtctaga
     7501 attttatgtg tattagtact actaaaaata taagctactt tcatttttat tccccctata
     7561 ctttttatat tttacaaaat tttacagata caattatatt ataagtttat tcttatttat
     7621 ctgtaaaata gatattaact atattattaa aagtgtatat agtatttatc tatttttatt
     7681 aaattattga ttgatttttt tctattttta ttaattttaa tgaataaaaa atagactcct
     7741 taaatttata aaacaaattt aaggagtcta ttttaattaa gtttatttca atataaaatt
     7801 tatatatttc tcaaattctt catacttata tcgagagctt taacagaatg tgtaagcgct
     7861 cctacagaaa caacatccac accaattttt cctatatctt ctatagtatc taaatttaca
     7921 tttccggaaa attctacaat ggctcttcca tttataatat ctactgcttg ttttgccatt
     7981 tccaaactca tattatccaa cattattata tctgcattgg cttctattgc ttcttttacc
     8041 atgtctaaag tttccacttc cacttctatc tttctaacaa aagaagtatt ttttctagct
     8101 aattcaactg ccttcttaac actaccagca gccccaatat ggttgtcttt aagcaatatt
     8161 ccatcagata aattaaatct atgattgcat cctccaccta ctttgacaga atacttatct
     8221 aatattctaa gattaggtat cgtttttcta ctatctaata attttgtatt tgtaccttca
     8281 agcttttcaa catacctact tgtaatagta gctataccac tcattctctg taaataattc
     8341 aatgctacac gctctcctac aagtaagttt ctagtactac ctgttaatac agctatcttt
     8401 tcacgattct ttactctatc accatcattt ttatataatt ttacatctac atcaccaagt
     8461 atctcaaaaa ctctcttaaa tacacctaag ccagctatta taccatcttg tttgcaaatt
     8521 aaatctacag tagatttaga attttcatcc acaatagaat tagttgttat atcttcactt
     8581 ggaacatctt ctatcaaagc atcctgtatc attctatcaa taatcaaata attcatcgcc
     8641 aaaatcctca cttgttcttt caatttcttt tatgactaat attttatttt ctttttttac
     8701 ctgttctata tctatatcca tatctggtac atctataaac ttaactttta cattatctat
     8761 aactgagttt atatttttag ctgctctttt tgaaaataca agcccttcaa gtaatgaatt
     8821 acttgccaat ctattggctc catgaacacc tgtacaacta atttcaccaa ctgcatataa
     8881 attttccata gatgtttttg aatccaaatc tacatgtatc ccgcccataa aataatgttg
     8941 tgcaggagat acctttatac attctttcgt tatgtctgtt cctctagcta aacattcatt
     9001 ataaataaaa gaaaatctat taattaaata ttcactatct aaatgtgttg catctaaata
     9061 cacataaggt aatttatctt tttccatttg ctcaaaaata gcttttgaaa ctacatctct
     9121 aggaagaagt tcatccacaa atctttcttt atttttattt aaaagtttag ctccctctcc
     9181 tcttaatgat tcagatataa gcattctcct accttcatta ttttcttcat aaaaggctgt
     9241 aggatgtatt tgaatatatt ctaaattttc tatttttata ttatttctta aagctattgc
     9301 aattccatca cctgtaagat gtctttgatt agtagaattt ttaaaaagcc caccaattcc
     9361 acctgtagct aaaactacta tttttgaaaa aacatgaatc tcttttttag atttaaaaat
     9421 tctagctcct atacatttat tgccatcttt taatatatct aaaagatatg catcttctat
     9481 aagagttata ttttctctag ttttagcttc ttttaataaa gttttaaaaa caacttctcc
     9541 agtattatcc ttactatgaa ctattcgatt tacactatgt gctccttctc ttgtgtaatc
     9601 tatctctcca ttttctttgt ctaaaggcat accatattct actatctgac ctacattttc
     9661 tatagattca tctgcaagta cttgaactgc ttcaactcta tttttatact gcccagcctt
     9721 cattgtatct tctacaaagg attctatatc atcaatattt ctagcagttg aaattccacc
     9781 ttgtgcaaga tatgtattgt tgttttcaat tgttgatttc gatactacta gcacatttaa
     9841 acttttatct aaatttaatg cacagtataa tcctgaaact ccacttccaa ctattaaaac
     9901 atcttgttct aaattcataa gtctatccct ctatccttct gctaatttgt gcatgttaag
     9961 cagtgaattt aaagcctttt ccatgatttc atcttctaca ataacttcat tttccatatt
    10021 tttaagtgta ctatataaat tttctaatgt tgtctttttc atatctgtac atagtatttt
    10081 tccaccagga atataaaagt ttttattagg attatttttc tttaattcat gtaatatacc
    10141 ttcttcagtt gctattataa agtctttgtt tttactatta gtagcatatt ttattattcc
    10201 tgatgtacta cctacataat ctcctaaatc tcttatttct tttttacatt ctggatgtac
    10261 taatacttct gcatttttat acttatcttt taactcaatt atttcttcag ctcttatatt
    10321 gttatggtat ttacaatatc cgtcccaaaa tataaagttt ttatctggaa attgttctgc
    10381 aatatatcct cctaaatttt tatctggaag gaatataatt tctttattat ctatgttttc
    10441 tagtatttta attgcactag aagatgtaac agaaacatca caatgtgcct taactttagc
    10501 tgtagagttt atataacata caaccaaagc atttggatac tgttgcttta attttatcac
    10561 gccttcttca tctaccatat ctgccatagc acacccagcg ttacttactg gcatcaatac
    10621 agttttttca ggtgataata tcttagcact ttctcccata aaccttactc cacaaaatac
    10681 aaccacttct tctttacaat ctctagcaat ctcacttaaa taatatgagt caccaactgc
    10741 atctgctaat tcttgtattt ctggtggttg ataaaaatgg gcaagtatta ttgcattttt
    10801 ttctttcttt agctctttga tttgatatgt taaatcttta tccataattg caccgctttc
    10861 tatatgtact agtgtatata cacctgtaaa cccattatat cacttgacta ttctttcttc
    10921 aagaatttta aacctcttaa tattgttaag atatagacat ttatattctc aattgatttt
    10981 ccttttttct ccatctattt aaactaattt ttatattttt taattttttt gtaaaatatt
    11041 atcagaattt tgtttatatt ctttattgct ataacattga aaatattacc aaatacctgt
    11101 atactaagta catggttatg tatatgtatt tatttctgaa attatattag ttgtaatact
    11161 ttatagaatt tgtaggttgt ccagaattaa attcaatata aatctacaca tttagataat
    11221 atgtatacaa agaccaaaaa attttataaa tggggtgata ttatgacaac attcttaatc
    11281 ggcttggcta tattactaat aggaggtgct ttatatggtg cttattgtga aaaagtattt
    11341 ggcccagatg acagaaaaac acctgcactt gctcaaagtg atggagtaga ctatgtccca
    11401 atgaaaaaat ggaaaaatag tctaattgag ttgcttaata tagctggtac aggaccaatt
    11461 ctaggaccta tccaaggtat attgtttggt ccaatagcat tcatactaat acctatcggc
    11521 tgtgtatttg gaggagcttt gcatgactat atgagtggaa tgattagtat aagagaaaaa
    11581 ggagcacaaa tgccttctct tatcagtaga tttttaggta ataaagtatt tcaagtctat
    11641 aatatattcc tttgtctttt aatgctttta gttggtgcag tttttattta tacacctggg
    11701 gatttagttg ttactcaaat tttaaatatg aaatcgacta taaataatcc agttgtctgg
    11761 atagtttatg gacttatctt tctatattac ttgtgtgcaa cactatttcc aattgataaa
    11821 atcataggaa aggtatatcc aatatttgga gctattctac ttttatctgc tgctggtgtt
    11881 ggaattggaa tctttacaca aggttatgat ttagctaatt taagtcttgc aaattggaaa
    11941 ggtattcatc ctgatggtat tcctcttatt cctacattct ttgttacagt tgcttgtggt
    12001 attgtttctg gattccactc aactcaagct actcttatag caagatcagt ttcaaatgaa
    12061 aaagaaggaa aaactacttt ttataatatg atgattttag aaggtttaat tgcaatgatt
    12121 tgggctgcag ctgctatggg tatttacaat aaaggaatac ctaaagaatt agtaggttct
    12181 cctgatgtaa taggtttggt tgcaagagat ttacttggtt ctattggtgg tatcatagct
    12241 ataataggtg taattgtttt accaataact tctggagata cagctttaag atctttaaga
    12301 ttaatgcttg cagattactt tcattatgac caaaaggaaa agaaacatcg tgttatattg
    12361 tcaatctgta tatttattcc tgtcatagcc atccttatat ttgcaaagct tagtgcttct
    12421 ggatttaata tattgtggag atacttctct tggagtaatc aaactattgc catatttgca
    12481 tttgcaatga ttacagttta ccttataata aaagaaaaga attatattat aagcttaatt
    12541 cctggtatgt tttattcctt tgttatattc agttatatat ttaatgctca aattggattt
    12601 aacttaaaca taaatatatc atatgtttta gctgccatat ttactgtatt atatgctatt
    12661 ttaactgtac gctctggtag aaagttaaaa agcaaagcag atactaagtt ggctgactaa
    12721 atctaaataa aataatactt agaataaatt ttattaaaaa atgataaaaa agtaggcaat
    12781 ctcaagttag atttgcctct tttttattta atacagtaat aaatacttaa gtatttactc
    12841 cttattggaa tatttatcct aaatctctaa tataaataaa taaaagtttt aaaataatct
    12901 attcatttat atacttaaaa taaatttttt ggaggaatac ttatgaataa taaattacat
    12961 gaacttgaaa aaaatctacc agaaatatct tgttcttttt gcacacatct ttcatttaag
    13021 gggcctagcc tagactatag atatgatatt agatgtgtta tttcagatac aaagcccgat
    13081 tttagaggat gttgtgaata ttttgaacca gaatatacag aattaaatac aggagattta
    13141 gacaatctat acataaactt tcttgagaca tgcctcaagg taaattataa tgattacatc
    13201 aattctatgt attggaagtt atttaaagaa agaaccctac tagaaaacaa ttttaaatgt
    13261 tctatatgtg gatcaactga aaatgttgat gtgtatcatg ttaataaaaa tttaggaagg
    13321 gaaacttcca aagatgtagt tgttaaatgt gataaatgta attgtaaata agtgtataaa
    13381 atttagataa cttcacataa taagtttaaa taaaacttag gaggttattt atgaaaaata
    13441 ataatttaaa ttgtgctgct actaattgtg cttataatac tagtggatat tgttatgctg
    13501 gtagtataaa agtagatgga atgcaagcaa ctactacaga aaatacttat tgtgcttctt
    13561 ttgaagacaa atatacatct ggtataacta gtcgttctaa tgaaactaac caagttgata
    13621 cagataacat tcattgtgaa gctgtaaaat gtaaatacaa taaaaatgaa ctttgcaaag
    13681 ctgaaaaagt tcatataaat gaaagaaatg caagttgtga aacttttgaa atgaaataaa
    13741 tttcttttta tttagtttta tagtatttat acaaagcaaa attgaaccta tcaaatagat
    13801 gtctttatta atgatgtcta tttataggtt cttttttaaa acaattcata atttatatat
    13861 aaaaataaat tattatttta atatattacc tagaagtaaa tttaacaccc agaatatcca
    13921 taaggaacat aaatattgga actccaatta gtaatcccca tacacctaaa taatgctctc
    13981 ctacaagtaa aataataaaa acaaaacaaa ctggaagttt agttctattc gacatcaact
    14041 tagggtttaa tatgtatgtt tctactgcat gcactataca aatcataagt aaaactccaa
    14101 aaacctttgt tattcctcct gtattaaatg ctattacagt taatggaatc aaagacacta
    14161 taactcctgc tactggaata agacccaaca caaaaatcat aaatcctaaa gcccatattt
    14221 gagggaatcc catgaaacct aagaaaatca tagaaacaac agaatttata aaggcaatca
    14281 taacttgtac tttcattact tcaccaaaat ttttaacaaa gctctttcca aaaaatacaa
    14341 gcgaattata aataaaagaa atcttactat cagaaagttt atctccaaaa tttttaattt
    14401 catttttttc aagtaaaagg agtaaactca gtaaaaatgc caataatata tttactccaa
    14461 aacttccaac ttttgttgct gttgatgcta taagtagccc tgcttgtgaa atatatttat
    14521 taaaatccag ctttgagaca ataggataca atttcggtcc taaagattga gcaaattgat
    14581 ttacatcaaa atcactaaaa accctactca actctgtaag ttgaactata attattggta
    14641 caactgcata gctaatcaat actaacaata tagcaaatag tgcatataac actataatta
    14701 tcaatgaatc tggaatattt aaagaagaaa acttcttaat tcgttttctt gtggctacaa
    14761 gcaagttata gaaaataaaa gtcataataa atgttaaaag caccatattt aacatatacc
    14821 aaaatgtaat tagaataatt actacgacag atatagaaac aattttatat tttgaactgt
    14881 cacgtgccaa tcctttgtta tcttccatag tttaaatcct ccccaaattt ttatgacttt
    14941 ataatgactg ccctttttat tttaatctca aaaaacaagt agaaaaatac taaaaataac
    15001 ttattaattt tcataaaaca atattaaaat ttagagaaga tacattttaa ataatcttac
    15061 ttatactgaa atctgctcta catcatatat ttaatttatt attatctatc tcatatagca
    15121 attcgtatat ctctggatat tcaaaaaaca aaccctcttt tagtaattgt cttatttcat
    15181 ctgtgcttac ccactttata tcacttactt cttcctcctg taaaatagct ttagatatat
    15241 catattcttt tttaactaag tatacatccc aaagagtatc ttcatgaatc atacttctaa
    15301 aaactttcat ctcatcttta gttatatcta tccctatttc ttcctttgct tcgcgaattg
    15361 ctccttctaa actgtcttcc ccagaaacaa tacatccagt tgtcattccc cacatatttg
    15421 gcaatgtttt tttactttta gaccttttct gaattaaaat ttgagagtta ctatttaaaa
    15481 tccacacttc aactgccaaa tggtaataac cttcttcaat agaattacct ctttttataa
    15541 catttcctgt tttaattcca tcagcattat ataaatccca taattccatt tcaaaatctc
    15601 cttgacttta ctataaatta gataaactac aaatatattt tagcataatt aattctgctc
    15661 attttttatt acaatacttg atttacattt atgttcattg cttttctttt ccttccattc
    15721 tcttatatca tctataataa atctatgttt cagcaatttt gtgtcaaatt taaatattct
    15781 aaacactatt tgtaatacat atcctaaacc taaagcactt acaagcgtac caatacctac
    15841 agaacctcct aataaccatc caacaactaa tgctagtatt tcaagaattg ttctaacaag
    15901 acgtacagat ttattagttt ttttctgaag agcaatcata agcccatctc tcggtccact
    15961 tcctaaacaa acacctaagt atagaaaact tgctatagct gccaaaataa ttcctataac
    16021 aaccataaac acacctatat aagtgttgct tgcatgaggt atcactccag aaaatattac
    16081 taaatcaata aaaattccta taaatgtcat gttgagtaaa gtaccccatc ctatattttc
    16141 accaaaaact gcatccaata taacaattac aactcctacc attatagaag cctgacctat
    16201 agttatgcct agctttatag ataaaccttg gtgaagtaca tcccaaggca taagacctac
    16261 atgtgcatta atcataagca cagaagaaat tgcaaatata aaaaaacctg aaaataatct
    16321 tatcatactt tttaaactta tttttcccat caaaaatcac tcctcataat aataataaca
    16381 ataaatacca ataaaatata tatataaata cgaaatttta cagaattatt tattaggtta
    16441 agataaaatt ttaattataa aaagtggcaa tccaaatttt atacattttc acctatactc
    16501 taagctttcg tgtataaaaa acggatatac cactttttta gtatataaac atttttagtt
    16561 tttcttatta attttcacta taaattctag cttcttcttc tccatttagt actcttaaag
    16621 ttccaaaagc aagtgattcc aactcatttt ctcctgccat aatttctact ggtgctataa
    16681 actcaacttt ctttttaacc attgaagtaa acatttcaga atatgcaatt ccacctgtta
    16741 taattatagc atctaccttt ccatctacta cagttgctaa ctctccaata ccttttgcta
    16801 actgataagc cattgcttca taaataattt ttgcttcttc atttccttct gctatcattt
    16861 tttcaacttc tctagcatct acagtattta ggtatgaaac tataccacct ttacctctta
    16921 tctttttagt catttctctt tctgaatatt gacctgaata acaggcagcc actaattttg
    16981 tagcaggtac tcttcctgac ctttctggag aaaatggtcc ttcatcatca gatagcatat
    17041 caaccattct tcctttttca tgaagataaa tacttactcc tccaccaatg tgagctacta
    17101 ttaagtttaa gctcttataa tcttttcctt tatccttagc atattttaaa gccattgctc
    17161 tagtatttaa tgcatgccct gcacttgatc tatccattcc acaaagtcca gatattctcg
    17221 ctacatctat aagctcatct actgcaacag aatcataaat atatgagttg attccaagag
    17281 gttctgatat tgcatgtgcc acaacagcac ctaaattgga agcgtgttca agtactggtc
    17341 tatgtcttag tacatcaatc atttcttcat ttactaaata tgctcctgat tttactggtg
    17401 gtaaaactcc acctcttcct actattgcac taagagattt taagtctata ctattttctt
    17461 taagaatatt caaaactgct tccttacgca tttcaaactg gtcttgtata gtattatatt
    17521 ttgcaatctc ttcagctgga tggtctatcg tcttcacaag aatttgttct tctccatcat
    17581 atactgctat ttttgtagaa gtagaacctg gatttatggc taatattctg taagtcataa
    17641 ttttcttcct ttcaccttaa tttctatagt ctatttatat ctattataaa ctatttgcta
    17701 accatttcca taccttcatg gaaagctttt atgtttaatt caacaaattt tggtttaaca
    17761 ttttcactta taattttttc ccaatcaata gactctaatt gcatagattt aactagacat
    17821 cctaataaaa taacattcat tatttttgaa tttcctagtt cttcagcttt atcagctgca
    17881 tttataactt ttgcatttaa tctacttaac tctgcatcta tatcactttc ttgatattca
    17941 gctcctccga ttaaaacagt catagaatct atcctatgat tatttactaa agcttttcct
    18001 tctggtttta aataattgaa ccatcttaaa gcttccattt tttcaaaaga tactaatata
    18061 tctgctccac caatttctat aactggagaa tgaacacaat ctccatatct aacttgtgag
    18121 gaaactgaac ctcctctttg actcattcca tgtatttcac tcatctttac atcatatcca
    18181 gcttccatca atcccatagt taataattta ctagcaagta tagttccttg acctcctaca
    18241 cctaccaaaa gtatactctt agtcatatta tttttcctcc ttaactattg cttgttttgg
    18301 acaaacttgt gcacatactc cacatcctac acactgattt ctatctatat ttgataactt
    18361 attctcttta tcaaatgata gagctggaca tccagttttc aaacataatt tacatcctat
    18421 acatttatca tggtcaactt tacacttagt cttaaatgga ttattaaatt cttctatatc
    18481 ttctttagag aattttttaa gtacacatgg ccatcttgtt attattactg atggttcatc
    18541 ttctatagct aaagcccaat ctagtgcttc atttacttct tttaaattat tagggtttat
    18601 aactcttaca tgttctattc cacaagcttt aactaatcct tctatatcaa cttctttagt
    18661 tttagcacct tgtaaagtat atcctgaacc tgggttttct tggtgacctg tcattccagt
    18721 tattctatta tctaatataa ccgaaataga attacctcta ttgtaaacaa catctaaaag
    18781 tccatttatt cctgtatgga agaatgttga gtctccaagt acacctacca atcttttttc
    18841 attatcatcc ttcatattaa gaactttttg agctccatgt gcagttccaa aagcagaacc
    18901 catacaaaca acataatcta ttccattata tggaggagca aatccaagag tataacaacc
    18961 tatatctcca ccaactatta aattttttct ctttccaagt tcatagaaga atcctctatg
    19021 tggacatcct gcacagaaac ttggtggtct tggtgttact aattcagatt tgtattctat
    19081 agtatcattt gtttttccaa atatagcttt tcttaaaaca tctggtgtca tttctccata
    19141 tggtggtatt acatcttttc caatacaatc tattccatat gcttttattt gttcttctat
    19201 aaatgggtca ttttcttcta taacatatat tttatcaact ttttctgcaa attcttttat
    19261 tttttcataa ggtaatggat ttgtaaatcc tagtttcata tatgatgcat tttttccaaa
    19321 aacttctttg gcaaaattac agcacatacc agaggctata acacctattt ttgtatcatt
    19381 tatttcatag tagtttaaat cagtttcatt tgagaatttt ttaagaattt ccattctttc
    19441 ttcaacaacg ccccttctta tttgagcatt tgctggaaca gttaccattt tcttagcatt
    19501 tttaacatac tctttaattt caacttcttc tctatcatag cactccacta atccttttga
    19561 atgacaaagt cttgttgtaa ctctatataa aactggtgta tcgtattttt cactaacttc
    19621 aaaagcttct tttatcatat ctttagcttc ttgactagtt gatggctcaa ataaaggtat
    19681 ctttgcaaat tttgcataca tcctattgtc ttgctcattt tgagatgaat gcattcccgg
    19741 ctcatctgct gtaataagta ccataccacc atttacccct gtatatgcat aagtaaatat
    19801 tggatcagct gcaacattca aaccaacatg tttcatagaa gccattgttc ttgctcctgc
    19861 aattgaaccc cctatagcag cctcaagtgc taccttttcg tttggagccc actctgcaac
    19921 tattgcatct ttatatgttg ctatattttc taatatttct gtacttggag tccctggata
    19981 tgcagatgca tatttaacac cagcctcata ggctccacgt gcaattgctt cattacctgt
    20041 catcaattgt ttcatataaa attccctcca tctacaatta ttaaattcat agtccaccca
    20101 atttgttcaa tgaaattaat tatttatttt tacttctaaa ctttcaagtg cttcttttat
    20161 aatacttggt gctttcttac atttatcttc agaaactgca caaactgcaa atcttaaacc
    20221 catttttaat ggaactgcaa ataaattatg tttagtcaat tcttcacata cttcttttgg
    20281 attatcacat ggtatactta caaagaaacc tgctatatat ggaagaattt ctaatcctac
    20341 tctttcagct tcttttacaa atacatctgc tcttctagta agcatgtttt tgtatatttc
    20401 tttttcatcc tcatattctt tgaatttttt agggtcattt acaatgttag ataatactgc
    20461 catggcactt ctattacaat ttgaccaatt tgctctacat gaatgaacac atgagtaatg
    20521 gaactcttca gctacatctt cacttgaaga tatacaaatt atagccccca ttctcatacc
    20581 ataagctgta aatccttttg acatactgaa tccaaccaat acgagaacgt tttcaggcaa
    20641 attagaaaat ttcttaaaaa actttctaca tacatcatca tcacctgcaa agtcaatata
    20701 tgctgaatct acgaaaaata ttatcttttt gtctttatct tttgcgactt cttttgataa
    20761 gtctaatatt ttatcccact catcatctgc aacactatat ccagttggat tatgcgctgg
    20821 tgtgtttata attgtaaaga ctctaccttg tttttctgct atatttacaa atttttcttt
    20881 aaacgactca aaattaaacc ttctattttc atcaaaaagt tggtaattaa ctacttttct
    20941 attagcctct tcagctatac ttacatatgg actccagaac caatcagatg ttaatacctc
    21001 atctccttca ttagtatagt tccatacagc taattttatt gcaccacttc ctcctggtga
    21061 agctaatact cttatatgtc cttctggtaa gtaatctctg aaaaatactt ttttaactgc
    21121 ttctaagtaa tctggttgac cttcaagagc tgcatatgca cctatctcac agttatctaa
    21181 tgctttatat tcttcatata cagttttcat tgttatcaat tttcctgaat catccattag
    21241 agcgcctatt gttgcattta ttacattatc cataccaaaa gccttctctg ctgcttgagc
    21301 tctgccagat atgctaaata taacatcatt ttcttttggc cacattgcat gcttaccaac
    21361 catactttga ctcataccat cacccctaaa attgtattta ctcttacatt atatattaag
    21421 caattactat gccaataaat cccactttat gtaaaattga ttttgtttag aattattatt
    21481 tttttcatta ttaaattaag tgttttaact atttttctta taaatatata tgcctgtcta
    21541 aaaaaataaa aatttgcaaa aaagtaatgt taatttttct taacacttgt tttaaaaaat
    21601 taacattact tcaaaataat ttatatgtaa ttatatttaa atatttttat tgtatttctt
    21661 ccataaagat gtcctgctta ttccaagaac ttgtgcaact tcatttttag aaagtccttg
    21721 agcaaaaagc atacttatta ttttttcttc aacatactta tttacttctt ttatatctat
    21781 acttatctta ccatcattta tcttacttat atttatagat tcatcttgga cttctacact
    21841 tgctgtcgtt tcagaattaa atttgtctac ttcagagtta ggaagtaatt ttttagtttt
    21901 aaaatctcta tcttgtctca atttaatttt acctgtcaca acatattttt gagctattgt
    21961 ctttaactct cttacatttc caggccagct ataattatat agcttatcca tttcatcttt
    22021 agtcaaaaca aaattttcat ctatactgtt tattccattg agaccatcat cctttaatac
    22081 ttcatttaca aaattgttaa acaaaggtat tatatcttct cttctgtctc ttaaaggagg
    22141 tatatttatc tctaaactac tcaatctata aaataagtct gctctaaatg tacccataac
    22201 tattttttcg gttaaagatt cgttcgttgc tgctataatc cttatatcca gaggtattat
    22261 ataatcagaa ccaattctca ttatttgacg ttcctctatt actcttaata gttttgtctg
    22321 aatattaaag gatagactat ttatttcatc taaaaaaaga gtccctccat gagcaagttc
    22381 aaatagccct ctttttccac cctttctagc accagtaaat gcaccttctt catagccaaa
    22441 aagttcactt tcaagtaaat tttctgctat agtagcacaa tttatagcaa caaaaggcct
    22501 atcttttctt ttacttatat tgtgtatact atgagctata atttctttac cagacccact
    22561 ttccccataa agtaaagttg tataatcact ttttccaatt tttttagctt cttcaataaa
    22621 ttctttagtt aatttatctt taaataaaaa atcatcaaaa gtatatctag catagagacc
    22681 tttttgattc aagtcaaatc ttatttttct ttctaagttt tgtagcttgg ttatatcttg
    22741 tattattcct aaaactccat aagtactatt atcaactttt atcaatgttg tcctagtatt
    22801 aacaattaga ttatttatat ttcttatctt tctatcttct acgtcctctt tttcatgtaa
    22861 acaatctaac atccactcca ttttaggaaa tacatctaag atgtatttat taagtgcaca
    22921 ttttctctct actttaagca tgttttttgc actttcattg tacaaaatta tactgccatt
    22981 actatctata gcaattactc catcttttat accatcaagt atatttcgta acacttcatt
    23041 attaaacttt tcttcaccta atgtatctaa taatttttta caatattcta cagcttctct
    23101 tatagattca tcactagcag ttgcaaatac actatcaatt ccatattgcc ttgcaaaaga
    23161 acatgccaaa cctcctccta cgattacaat ttcatctttt tggtctatat attttacaac
    23221 tttacttctt atctcatact tagattcaaa ccattcttca gttatttcag tacttatgac
    23281 atttctccag ccaacatagt caaaagaaac ttcattgtct ccaagaatca atactacttt
    23341 tttagaatat ttctttgcta tttctattgc tcttaaaagg tcagttgact tcatattcat
    23401 tggtataaca ggtacattta ctgtatctat tactaaacta taacctccac ctctaccaat
    23461 tatagcttgg gcaccttttt caacaagtat acgaccttga ttttccatta agtctaggtt
    23521 aagtatgtcg attattatag ttccattttc tacatcttct ctatataatt cttctattct
    23581 ttcttttaat tcaatatcag aggctataat tcctattttt tttcgcaaca aaatcacctc
    23641 aattttaaat tataatgtat tgatttctac ctgctttttt tgatttatat aggttcttat
    23701 ctgcatcttc aattaatatt tcaacatctt ttctactacg acatcttttt atacttactc
    23761 cagcacttat agttatatag ttttttattt tagaatatat atgttcaata ccctcaaatt
    23821 caagaagttt gaaaactctt ttaatatata attctatatt atcaatctct tcaacaccga
    23881 ttgattctat agaatctact aatattaaaa tttcttctcc cccatatctg attatgtgtt
    23941 cggtaatatc tataaataca cttcttataa tatcagttac cttaactata actttatctc
    24001 ctgctagatg accataatta tcattataac ttttaaaaca atctatatcg accagtattg
    24061 cgaccatatt tttgtctctg gtttcattaa ataaatccaa ctgctgttcc agatatcttc
    24121 tattatatag tttagtaagt ccatctttta aaatatcatt ttctaatttc ttattatcat
    24181 ccactagtaa tcttaaactc tcttgttttt gtctgagttc atattgtttg tgaattgctt
    24241 gagatgtttt ataggaatta tttttttccc acctatcaga ataccaatcc atacttttat
    24301 aatattctat tgccagttta aaatcttttt ttagctcata tccatttgct attagcccat
    24361 aaaatataga tatttttcta agattcaatg aactcttttt aactaaattt aaacctttta
    24421 tataacaact tataccttca tctatattac caaattttat atataaatct ccatacaact
    24481 tatatatgta atctagttta gaattatata taaaaacatc tccaaaactt ttatatatat
    24541 tttttgataa aattaaatag gtgtttacct tttcaaaact acaattatct ttctttaact
    24601 caatttcagc tgaatataaa aatataaatg aatataaata atccttatat acaccttcaa
    24661 caactttcat taattttata gtctttaata aaataacttc tgcttcttta ttcatatctt
    24721 cttttaaata tgcccatata aggtctattt ttatagacac tctttcagtt actgatattg
    24781 agttattact gtgtgcaa
//
LOCUS       DACULQ010000043        24698 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.043, whole genome shotgun sequence.
ACCESSION   DACULQ010000043 DACULQ010000000
VERSION     DACULQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.043"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..178
                     /locus_tag="JA154_06955"
     rRNA            <1..178
                     /locus_tag="JA154_06955"
                     /product="23S ribosomal RNA"
     gene            298..414
                     /gene="rrf"
                     /locus_tag="JA154_06960"
     rRNA            298..414
                     /gene="rrf"
                     /locus_tag="JA154_06960"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            534..725
                     /locus_tag="JA154_06965"
     CDS             534..725
                     /locus_tag="JA154_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAT7995604.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            880..2289
                     /locus_tag="JA154_06970"
     CDS             880..2289
                     /locus_tag="JA154_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAT7995605.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNILKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            2420..3109
                     /locus_tag="JA154_06975"
     CDS             2420..3109
                     /locus_tag="JA154_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAT7995606.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            3112..4047
                     /locus_tag="JA154_06980"
     CDS             3112..4047
                     /locus_tag="JA154_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAT7995607.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMNGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            4044..4937
                     /locus_tag="JA154_06985"
     CDS             4044..4937
                     /locus_tag="JA154_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation protein"
                     /protein_id="HAT7995608.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            5015..5761
                     /locus_tag="JA154_06990"
     CDS             5015..5761
                     /locus_tag="JA154_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7995609.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5762..6595
                     /locus_tag="JA154_06995"
     CDS             5762..6595
                     /locus_tag="JA154_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAT7995610.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFIFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6618..7322
                     /locus_tag="JA154_07000"
     CDS             6618..7322
                     /locus_tag="JA154_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995611.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLKTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7510..7938)
                     /locus_tag="JA154_07005"
     CDS             complement(7510..7938)
                     /locus_tag="JA154_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAT7995612.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7975..9405)
                     /locus_tag="JA154_07010"
     CDS             complement(7975..9405)
                     /locus_tag="JA154_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAT7995613.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9537..10121
                     /locus_tag="JA154_07015"
     CDS             9537..10121
                     /locus_tag="JA154_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HAT7995614.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            10137..10658
                     /locus_tag="JA154_07020"
     CDS             10137..10658
                     /locus_tag="JA154_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HAT7995615.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10724..10970
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11054..12991
                     /locus_tag="JA154_07025"
     CDS             11054..12991
                     /locus_tag="JA154_07025"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090061.1"
                     /note="methionine--tRNA ligase; MetRS; adds methionine to
                     tRNA(Met) with cleavage of ATP to AMP and diphosphate;
                     some MetRS enzymes form dimers depending on a C-terminal
                     domain that is also found in other proteins such as
                     Trbp111 in Aquifex aeolicus and the cold-shock protein
                     CsaA from Bacillus subtilis while others do not; four
                     subfamilies exist based on sequence motifs and zinc
                     content; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAT7995616.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            13021..13791
                     /locus_tag="JA154_07030"
     CDS             13021..13791
                     /locus_tag="JA154_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase TatD"
                     /protein_id="HAT7995617.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            14128..14559
                     /locus_tag="JA154_07035"
     CDS             14128..14559
                     /locus_tag="JA154_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAT7995618.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14574..15149
                     /locus_tag="JA154_07040"
     CDS             14574..15149
                     /locus_tag="JA154_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAT7995619.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            15152..16237
                     /locus_tag="JA154_07045"
     CDS             15152..16237
                     /locus_tag="JA154_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase"
                     /protein_id="HAT7995620.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            16257..17126
                     /locus_tag="JA154_07050"
     CDS             16257..17126
                     /locus_tag="JA154_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnJ"
                     /protein_id="HAT7995621.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            17119..17985
                     /gene="phnK"
                     /locus_tag="JA154_07055"
     CDS             17119..17985
                     /gene="phnK"
                     /locus_tag="JA154_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAT7995622.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            18026..18739
                     /locus_tag="JA154_07060"
     CDS             18026..18739
                     /locus_tag="JA154_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAT7995623.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18740..19909
                     /locus_tag="JA154_07065"
     CDS             18740..19909
                     /locus_tag="JA154_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAT7995624.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19913..20749
                     /locus_tag="JA154_07070"
     CDS             19913..20749
                     /locus_tag="JA154_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAT7995625.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20750..21745
                     /locus_tag="JA154_07075"
     CDS             20750..21745
                     /locus_tag="JA154_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HAT7995626.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21763..23451
                     /locus_tag="JA154_07080"
     CDS             21763..23451
                     /locus_tag="JA154_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HAT7995627.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGTSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23463..24530
                     /locus_tag="JA154_07085"
     CDS             23463..24530
                     /locus_tag="JA154_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT7995628.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDVMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9829 a         2777 c         4429 g         7663 t
ORIGIN      
        1 tgtacggaat ggataagcgc tgaaagcatc taagcgcgaa gccaacttca agataagatt
       61 tcccaccgca agggtaagac cccagaaaga ctatctggtt gataggtcga aggtgtaagt
      121 gcagcaatgt atttagctta tcgatactaa taggtcgagg acttgaccaa tatttatttg
      181 atgttcattg attaagatat atgtgtagtt tttagagtgc taactctaaa gaaactagta
      241 ttactaggtt ctataaactt attaaaaact taatagaaat attaagcata aagattatgt
      301 ggttacaata gcagagagga tacacctgtt cccattccga acacagaagt taagctctct
      361 agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtaatttt
      421 ttttattttt tgtcaaaaaa aacaaaaaat acgtatttct tttttcattt cgggaaaact
      481 aaacacagga attatggact ttataaaaat ttaattcttg aggagtgata taagtgaaaa
      541 aagttgcttt aattttagct ataataggtg cgcttaactg gggtgcaatt ggtattctag
      601 gtacagactt aataggtagt atctttggtg gaacatatga aatggtaagt agaataatat
      661 acttcattgt aggtttagct ggattatact taataccaag tttaatgtct gatgatagag
      721 aataatttat gcttttatgt taaatacctc attctaaatg tttagaatga ggtatttaat
      781 tttataaaaa tattaatttg tttctttttt aatggtaaaa tagataagaa ttgaagtttt
      841 aaaaagaagt attatgtaaa aaaggagtac tattaatata tgtttattat taatgaatta
      901 aataagattg taaatgaaga agtaatatct tttcatatgc ctggtcataa aaagggacag
      961 gtttataaaa agttaggata taaagatata ctaaaaaatt tgtataaaat ggatacaact
     1021 gagatattag gaacagataa tttacacttt ccggaaggaa ttataaaaaa ttcacaaaaa
     1081 aaagcatcaa gagtatttaa aagtgattat acttgctatt tagtaaatgg tagtagctgt
     1141 ggaatacaat ctgcaataat gtcagtatgt agtcctaaaa gtaaaataat aacaaataga
     1201 gactgtcatc agtctgttat aaatggatgt attttaggag atatagatat agagtatgtg
     1261 ccttgtgaga taagtgaaga gacaaatatc ttaaaaggga tatctataag taatgtgatt
     1321 gatataatag ataaaaactt ggatgcaaaa gcagtactgc ttacttatcc tacatattat
     1381 ggaatgacat atgatttaga atacatatgt aattatgcac atagcaaaaa gatggtagtg
     1441 atagtagatg aagcacatgg tgcgcattta gggcttagtg agaggcttcc taaaacagct
     1501 cttgagcaag gtgcagatat agttgtacag agtacacata aaactcttcc agcatttaca
     1561 caatcgtcaa tgatacatat acagggagac cgagttgata tagataaaat attgaacatg
     1621 ttaagaatta cagaatcttc aagcccttca tatttattat tatcttctct ggaattagca
     1681 gtagatatat atgaaaataa aggaaaagaa ttaatggaag agcttttaaa taatattata
     1741 actttcaaaa ataaagttga ttcaaatatt aatatatacg acacaaatga taaaacaaaa
     1801 atatttatat catctaaaaa catgggttta acaggttatg aactagaaaa tattttgaga
     1861 aataaatata acatacaagt ggagttatct aattattatg gagttctttt aatatgtaca
     1921 ataggaaata ctgctgagga tttctctagt ttagaaacag cattaaacaa tgttgtaaag
     1981 gaagacttta aatttgaaaa gttagaaaat aaaaaatacc ctgttgttat tccaaagaaa
     2041 atattaacac ctagagaagc attttataaa actaaaaaaa gtgttaaaat atatgacagt
     2101 ataggtaaag tttgtggtga atgtatagtt ccatacccac ctggaataag tgtaatatct
     2161 cctggtgaag taataagtaa agaaataata gactatctaa tgttttgtca ttctaaaggc
     2221 atgattgtga gtggattgaa agatgtagat ttaaatttta tacaagtaat agatttagaa
     2281 gacatttaaa aattatgata taaaagtaga tagaagtaga tagtttaaaa ttgtgaagag
     2341 aaataaatag actgcttata tagagatatc taataaaagt actaaaaaag taaaaagtgt
     2401 ttgtatggag gatatgaaaa tgaaaggtaa gttaataatt atagagagtg gttcagatgc
     2461 aagtggaaag gctactcaga cagaaatatt atatgaaaga ttaaaaaaag aaggctatat
     2521 gattaaaaag gtagaatatc caaattatga atctccatca tctgccttgg tgaaaatgta
     2581 cctaaaagga gactttggta aaaatgcatc agatgtagac ccatatatag catcaacttt
     2641 ttttacagct gataggtatg catcatttaa aaccgattgg gaggagtttt acaatgaagg
     2701 tggaataatt atcgcagata gatatacaac ctctaatatg gtacatcagg cctctaaaat
     2761 ggatatttgt gatagagaag agtatttaaa ttggttagct gattatgaat ttaatttatt
     2821 taagatacca caacctgatt gtgttatatt tttagatgtg cctattgaat ttagtaaaaa
     2881 gcttatggag aatagaaaaa ataaaattac aggggaagaa aaaaaggata ttcatgaaag
     2941 tgacatagag tatttagaga aatcttacaa caatgcttta tatatagcag ataaatatga
     3001 ttggaaaaaa ataaattgtg ttttagataa caaattaaga agtattgaaa gtatacataa
     3061 tgaaatttat aaaatagttc ttgattcaat aaaacctatg gagagataaa tatgtatttt
     3121 gaaaatatta tagggcaaga ttttgcaaag aaatatttaa caaattctat aaagaaaaat
     3181 aaacttaata atgcgtatat gtttgaagga atggatggaa taggaaaaaa gaaatttgca
     3241 gatgaattat caaaactatt acttgattat gaaaatctcg aaaatagtcc agactatgta
     3301 ctcataaaac cagatggaaa tagtataaaa atagctcaaa tcagaaattt acaatcagat
     3361 atagtaataa gacctcataa agactataaa atatatataa tcaataatgc tgaaaaaatg
     3421 actgtggaag ctcaaaatgc acttttaaag acgctagagg aacctccaaa ctatgctata
     3481 ataatcttag ttacaaacaa caaggaatct ttattagaaa ctataaaatc tagatgtgat
     3541 ataataaagt tctcacctat accaattgaa gacttaaaac gttatttgat aaatacaggt
     3601 atagaagaag aaagagctca attattggct attttttcga gaggaagtat tgagaatgct
     3661 ctaaatctat ctcagtcttc tgagttttca atgatgagag aagatataca acagtatatt
     3721 cagataatgt tagataaaaa catagtggag attttaaata taccaaataa tatggaaaaa
     3781 tatagaggta agattatagc tttattagat atgatgataa actattttcg agatatcatc
     3841 ctattaaaag aaaatgtaaa caagaacatg ttaataaatg tagataagct agttttcatt
     3901 caaaatatga atggaaaaat tagttattct caattgtcca agattattga tataatagaa
     3961 gatgcaaaga gtaaaataaa aagcaattgc aatttcaata taagcataca agttatgtct
     4021 ttaaatatat acgaggtgat taaatgataa agatagtagg tataaggttt aaaagtgctg
     4081 ggaaaatata ttattttgac cctgttgatt ttaatataga gcaagatatg gatgtagttg
     4141 tagaaactgc aagaggcttg gagtatggga aagtagtcgt tggtccaaaa gatatggatg
     4201 aaagcgaatt agtttctcct ttgaagccaa ttataagaat agctacagaa gaagataaaa
     4261 aagtatatct agaaaataag gaaaaggcaa aagaaacttt tgagttgtgc caacaaaaaa
     4321 taaaagaaca tgagttaaca atgtttttaa tagattgtga gtatacattt gataggaata
     4381 agttgatttt ttactttaca gcagaaggaa gaatagattt tagagaattg gtaaaagact
     4441 tagctgctat atttaaaact agaatagaac ttagacaaat aggtgtcaga gatgaagcta
     4501 aatctatagg aggtctaggg ccttgtggta gaaaactttg ttgttcatct tggttaggtg
     4561 attttcaacc agtttcaata aaaatggcaa aagaccaaag tctatctcta aatccaacaa
     4621 aaatatctgg aatatgtggg agattattct gttgcttaaa atatgagcat gatgtatata
     4681 gtgaagcaat agaaaaaatg cctgttgtag gtgcattggt gcaatcagat gatggaaaag
     4741 gaaaagttgt agaagtgaat cctttattag aacaaattaa agtggaattt caagacaaga
     4801 cagtaaagat atacacaaga gaagatataa aaatacttag agagcctaaa aaatgtgatg
     4861 gctgtggtgg taaatgtaag gataatgatg gattagatga agccacacta agagagctaa
     4921 agaaattaga agattaaatt taaggtaggg cttttcctac cttttttaaa gcaattaatg
     4981 cttaaagtta ttaatatttg aggagaattt atatatgcaa ttaaaagatt cagaaagaat
     5041 agatgattta caattaaaag gccttaaaat tatacaagat actaatggtt tttgttttgg
     5101 tatagatgca gttttattag ccaattttac taaaataaaa aaagatgcca aagtagtaga
     5161 tttgggtact ggaactggca ttatacctat tttgatagct ggaaaaagtg aagctaaaaa
     5221 aattattggc gtggaaatac aagaagatgt atatgaaatg gcaactcgtt ctataaaatt
     5281 aaatggatta gaagaaagag ttgagattat aaatgaagat ataaaataca tagacaaagt
     5341 cttagacata aatggttacc atgtagtcac atcaaatcct ccatatatgc atattgatgg
     5401 aataaaaaat ccaaatgata aaaaagcaat atcaagacat gaagtaaggt gcaatttgga
     5461 agatgtaatt agagctgcat ccagattggt tatgcctaga ggaaaattct tcatgataca
     5521 tagaccaaca agattggttg atataataac tttagggaga aaatataaat tagaaccaaa
     5581 ggttattcaa tttgtgcatc caagacctca aaaagcaccg aacttagttt tggttcaatt
     5641 tgtaaaagat ggaaggccag agcttaaaat attggaccca ttgtatgttt atggagaaga
     5701 tgggaattat acaaaagaat taaaagcaat atataataat aaagatatag gagagttgta
     5761 aatgagcggg aaattgtaca tatgtccaac accaatagga aacctagaag atataacata
     5821 tagaacttta agaatattaa aagaagtaga cttgattgca gcagaagata caagacatag
     5881 tataaaactt ttaaatcatt ttgaaatctc aaaaccacta actagttatc atgaacataa
     5941 taaggattca aagggagatt atttgataaa taaactaatt gatggtgaaa atatagctct
     6001 gataagtgat gcaggtatgc ctggtatatc tgacccagga gaagagataa taaaacaagc
     6061 tatacaaaat aacatagaga tagaagtatt gccaggtgca acagcctttg taacggcttt
     6121 agttggctct ggtatggaca cacatagatt tatttttgag ggctttttag atagagataa
     6181 aaaagttagg aaagcacaat tggaagaatt aaaggaagaa agtagaacta taattttcta
     6241 tgaatcacca catagattga aggatacact aaaagatatg cttaaaattc ttggtaatag
     6301 gaatatttct ataaatagag aacttacaaa aaaatatcaa gaaataataa gagaaaatat
     6361 agaaactacc ataaaaatat ttgaggaaaa agaagtaaga ggagagtttg tattaatagt
     6421 agagggtttt catggtgaga agtcaaagaa agaaaattat gatgggctta cagataggga
     6481 atatgtttta aaactaattg aaagtggtat gaacaaaaaa gatgcaataa aaatagtttg
     6541 taaagataga aagttaaaga aagatgtggt ttataaacaa gttttagatt tataaaacct
     6601 gaatctgagg tgattttatg gaaataggga aaattataat agagagttta aagagggaag
     6661 atacacataa aatatttata gaattagata ataagcatga attagaaaaa ataattccta
     6721 aaataagctg tatgaagtct gtaggtgaat gtaaatatca tgtagtaaat tgttttgaac
     6781 attctataaa tgcattaaaa gaattagaaa tagtattaaa tgataaggat ttttttccaa
     6841 tacatctaag aaaacatgtg tcaaactatt taaaaacata tattgaagaa ggaataaata
     6901 agttacatgt attaaagctg ggaacatttt tacatgatat aggtaagcct gacagtatga
     6961 ccttagatga aaccggaaga gttcatttca caaatcatga aaaaataggt gctcaaataa
     7021 ttgacaatat gggaataaag ttagagttat caacagagac atatagactt ataagtaaat
     7081 atgtaagata tcatatgata cttttgtctc tatataagaa aaatgatttg agtaggaaag
     7141 aattaattaa tgtatttaac ttagtagatg aggacactat aggtgtaata atgcttgggt
     7201 atgcagatat agtgtctaca gtaaaattat tgggcaagag tggagaagtc agtgtgttaa
     7261 agacatatat ggaatatatt ttaacaaatt acttgtataa gagtaattat acacatgtat
     7321 aaatttagtt aatataattg agttttagat gattaattga agtttgaaat atacggattt
     7381 caaaaacata aaagaagttg tctttgttgt aaaattatga aagacaactt tttttatatc
     7441 aaaaattgaa attttattaa ggatacttaa tggtctatta attaataaac cattagtatt
     7501 taatttatat tacaatattg attttgcttg tattggcgtt gatagtataa cagaggtctt
     7561 agtagaacca attttcttta tagtatcaat cacattttct agctcgtaca tatcttttac
     7621 aattactttt aaaagtaagc aatcatcacc tgttatatgg tgacattcta caatcctagg
     7681 gtcctttgca gctgactcaa taaattctgt atatccattg cttggaagag aaatatgaat
     7741 aaatgccttt ataactctgc ctaatgaatc tgggttgaca atagctttat atccttctat
     7801 aacaccagac tcttctaatc ttttgactct ttctgaaact gcaggagaag ttaaaccaac
     7861 tatttttcct aagtccttca tagaaattct cccatcatcc tgtaaaattt ctatgattct
     7921 gtaatctgta acatccataa ataaacagtc ccccttaaat aaaaataatt aatgctataa
     7981 aactttttta gaatctatca tttgtgtttt taaataccaa agatttctag ataaatcaac
     8041 cttataaatg tgtggttttg aaagtctttt gtattgttcc cagatagtac ttaattcata
     8101 ttgtacatga ttttttattt caagaacagc ttttttatta tatttgcatc tgccttttat
     8161 atacaatggt ttaagaagct ctttaacttt gtaatttgta aataccttcg ttttccatgt
     8221 ataagtagga tggaagattt ctaaaggttt agactcatct ataacttcat catgtaacat
     8281 aatcaaatct gcctctgcct tattgtcttc gttatatatt cttataactt tcttgtaacc
     8341 aggattattt attttttctg gattctcaga aatctttatc ttaggttcaa gaactccttt
     8401 ttcacaagat gcagctaatt tataaactcc tccaagagaa ggtgaatcga aagatgtaat
     8461 gagctttgta cctactcccc atgaatttat agtagctcct tctgctttaa gtgatgttat
     8521 ggtatattcg tctaggtcat tagatgcagt tatgctgata tctgaaaagc cagcattatc
     8581 taattgtttt ttagcttctt tagataaata agctaaatct ccagagtcaa gtcttatacc
     8641 cataggtttg tatccttttt cagaaatatt tttgaatact tttattgcat taggaactcc
     8701 actgtttaat acatcataag tatctacaag tagtaggcat ttatctggat aaacatcagc
     8761 atatgcttgg aatgcttcaa gttcattatc aaatttttgt acccaactgt gagcttgtgt
     8821 accaattata ggtatatcaa acattttccc tgctagtacg tttgcagttc cagcacaacc
     8881 accaactacg gcagccctag ccccatatat ccctgcatct ggaccttgtg cacgacgtaa
     8941 tccaaattca aatacagggt ctccttgagc tgcaaagcaa actctagatg ctttagtagc
     9001 aataagagac tgaaaattaa ctattgttaa caatgctgtt tcaataagtt gagcttgata
     9061 taaaggtgcc ttaacagtaa ttaatggctc attagggaac atgatagttc cttcctcaac
     9121 agcatatata tctcctgtga atttaaattc ttttaaaaat tttaagaact tgtcagaaaa
     9181 taaatttaag ttttttaagt actctaaatc ttcatctgaa aaatgaagat tatctatgta
     9241 ttcaacaacc tgatcaatac cacaaactat agtataccct ccatcacaag cgttttttct
     9301 aaaaaacata tcaaatacaa caatgtcttc atgaatattc ttttcaaaat agccatttaa
     9361 catagtaagc tgatataaat cagtaagaag tgttagattt ctcattattt ctctccttaa
     9421 ttattattaa atattaaaaa ttgtaaaaac tcccacactt atactaaaac ataatatagt
     9481 acaactttca agtataaagt attacctaac tatcataaaa ttatttaaag aggaggatga
     9541 ttataatagg aaaaatatgg ttttatatgg tatcgatagg aataatagga agcttgattt
     9601 ctaaaaatat gggtgaatta aataaagtta tattaagtga aacatcaaaa ggtatagagt
     9661 ttgcaataag tctagctggt atcatggcac tctggatggg gataatgaat atcgcaaaag
     9721 aatcaggttt aattgaaaag ataggtcaaa aactaaatcc tataatgaga agattatttc
     9781 cttctattcc aaaaggtcat aaagcaatgt cttatatagt tatgaatatt gctttaaata
     9841 tgttgggtgc tggaaatggt gcaacagcat ttggtttaaa agcaatgagt gagcttcaaa
     9901 ctttgaataa caagaaagat acagccacta atgatatgat tatgtttatg gtaatcaata
     9961 tatcatctat acaaataatt ccatttacta taatcaaact aaggatggat atgggggctc
    10021 aaaatccaag tgaaataatt tttacgactt tatttgcaac cataatatct acagcagtag
    10081 caataataac atgtaaattt tttcaaagaa gatatagata aaggggatgt agatacatgg
    10141 agatattttc aaacttactt ataccaataa taattctgta catagtagtt tatggtaaat
    10201 ataaaaaaat agatgtgtat gatagttttg taaaaggagc aattgatggg ttaaaagcag
    10261 catgggatat cctaccatat ataataggta tatttttggc aataggaata ttcaaaactg
    10321 gtaaaggtct tgatatgtta gaatggctat ttacaccaat agcaaatatg atgagtatac
    10381 caaaagaatt aataggtcta ataagtgtaa aacctttatc tggaagtggt gctttaggga
    10441 tgtacagtga acttgcaaat agagttggaa taggaagttt agtagaaaaa atgggtgcta
    10501 cgattgtagg ttcttctgag actatttttt acacaatggc aatatactat ggaagtctga
    10561 agataaaaaa tactagacat acattatcat gtgctatgat tagccatgtt gcaggtgtca
    10621 tagcagctgt ttttatttgc tatgtaatat ttgtataatt tgttgacaat atgggaaaaa
    10681 ttttataata tagtgattat atagaaacaa atagtaatac aaattacaag ctatgaaaag
    10741 aagagtaaat aaaggttttt tctatcagag agctaggtta ggtgaaagct agtgtcaaaa
    10801 gctttattga agatggtctt ggagcttttt atctgagcaa gtgtgctagg gtaaaacggt
    10861 tggacacgtt atagtctact aaggtatcta taaaatgttt ctacatttca tgtgaaacat
    10921 aactattata gatgaattag ggtggtaccg tgaataatct cgcccctatg ttaactcata
    10981 ggaggcgatt ttttattatt taaatgaaag aatcatatga ttacgaacga taaaaaatta
    11041 ggaggtaaat aacatgagca aaccgagttt ttatgtaaca acaccaatat actatccaag
    11101 tggaggatta cacataggac atacttattc aacagtagct gcagatacaa tagctagatt
    11161 caagcgtttc tgtggatatg atgtaaagtt cttaacagga acagatgagc atggtgaaaa
    11221 aattcaaaag aaagccatag aacaaggaat gtcagagata gagtatcttg atggaatgat
    11281 aaaagatata aaggccttat ggaatactat ggatatatct tatgatgatt ttataagaac
    11341 tacagaaaaa agacatacag acataataca aaagatattt actaaactat atgagcaagg
    11401 tgatatatat aaaggtgaat atgaaggtag atactgtact ccttgtgaga gtttctggac
    11461 agaatctcaa ttactagaag gaaataaatg ccctgactgt ggaagagaaa catatttagt
    11521 aaaagaagaa tcttatttct ttagattatc taaatatgaa gacagattaa aagagttatt
    11581 taaagatgat agtttctgtt ttcctgctgc tagaaaaaat gaaatggtgg caaacttttt
    11641 agacaaagga ttagaagatt taagcgtaac tagaacgact tttgactggg gtataaaagt
    11701 tccttttgat gaaaaacatg taatttatgt gtgggtagat gctttatgta actatataac
    11761 agcattaggt tatatgactg ataatgatga agaatttaaa aaatattggc ctgccaatgt
    11821 tcaaatagta ggtaaagaaa tagtgagatt ccatacaata atatggccag cgcttttaat
    11881 ggctttaggt ctagaagttc caaaacaagt atttggacat ggatggatac tatttgctga
    11941 tgataaaatg agtaaatcaa aaggtaatgt ggtttatcca gaacctataa tagaaagata
    12001 tggaatagac acacttaagt acttcttatt aagagagttt gcatttgggc aagatggaag
    12061 ttatactcat agaaattttg taacgagaat aaattatgat ttagcaaatg atttaggaaa
    12121 cttaataagt agaactgtag ctatggttga aaaatacaat aatggtataa taccaacagc
    12181 aaaagtttca actaattttg atgctgactt aaaagaacaa gctgtttcaa ctagagaaaa
    12241 ctttgaagct gaaatggata aaatgcaatt ccatgaagca ttagagagtg tatggaaatt
    12301 agttagaaga actaacaaat atatagatga aactatgcct tgggcactag ctaaagatga
    12361 aactaagaaa gatgaattag acacagtttt atataattta tgtgaatcta taagaataat
    12421 cgcaacacta ataaatccta taatgaatga gactgctaat aaaatatatg aacatatagg
    12481 aataaaaggt caagatgata taactacatg ggaaagtaca aaaacatttg gtcttattgg
    12541 agaaaatgta aaagtattta aaggtgaacc tttattccca agattagatg tagaaaaaga
    12601 gatagaagaa ttaactaaaa tgttctctgg gaaacctcca gtagaagaaa aacctttaga
    12661 gcataaagaa gaaattacta ttgatgattt agacaaaata gagcttagag tagggaaaat
    12721 aataagctgt gaaaaacatc caaaagcgaa taaattatta gtatcacaag ttaagattgg
    12781 accagaaaca agacaaatag tatcaggaat agctgaatac tataagccag aggatttagt
    12841 aggaaaagag gttacagtgg tatgtaactt aaaaccagtt aaattaagag gtgtagaatc
    12901 tcaaggtatg atattagcag caggtgatga tggagaccca tatgttttac catttacaca
    12961 aggggctaaa gatggatgtg aggttcgtta gtaggtaatt aaatagtaag gagattaaag
    13021 atgttatttg actcacatgc acatttaaat gatgaaagtt ttgatgaaga tagagatgaa
    13081 cttattggtt cattaaaaga taaaggtgta gatttggttg taaatccagg agcagatata
    13141 gagacgtcga taacagcaat tgaacttgcg aaaaaatacg actttatata ttcagcagtt
    13201 ggggtacatc cacatgatgt atcaaaacta gatgatacag caatagaaac tctaagaaaa
    13261 cttgcaactg agaatgaaaa agttgtagct attggagaga ttggattaga ttattattat
    13321 gattattctc caagagaaga acaaaaagag tggtttaaga agcaaattga attagctaat
    13381 gagctaaaac ttccaataat aatacatgat agagatgcac atggagatac ttttgagata
    13441 ataaagaata ctaaaaatcc tgaaatagga tgtgtacttc attgttatag tgggaatgta
    13501 gagttagcta gagaatatgt taagatgggt tgttatatat caattccagg tacggttact
    13561 tttaagaata ataaaaaaac tagagaagta gtaagagaaa tacctctaga aagattattc
    13621 atagagactg attcaccata tatgtcacca gagccacata gaggaaagag aaacaatcct
    13681 tctcaagtat cttttgttgc tgataagata gcccaggaaa aagggatatc ttatgaagaa
    13741 gtatgtagag ttacaaaaga aaatgcaaag aaatttttta atataaaata gacaaattaa
    13801 atcttataat gatatatata ataagtcatt ataagtatag gatgagagtg cctcaaaatg
    13861 agttttttag tttatgaggc actctttttg tatgaaatca aatatatttt catcatttca
    13921 acaatgaaaa agaagtttat catattttga gatagtttgt ttttatttat ttaattcata
    13981 ttttaaaata tattttctta acattgttaa aaaagaattt acaagatatt cattagaatt
    14041 aactcaactc taatattcac ttaacttaag ttgtttatta tattattaaa gagaagtaaa
    14101 cgaaaagtag attaaggagg ttgaattttg tatacagttg atagtactaa gatattgata
    14161 aatatgaact tagagaaact agaaaagata tactcaatga taaaagatac atcaagtatt
    14221 tctataatta aagaacctaa cttggctaca gtaatggtaa gagctaatga aagtgtaaaa
    14281 aacacaacat ttaatttagg agagatatta gttactgaat gtagtgtaaa ggtagatgaa
    14341 agcttgggtt acggaatagt ttcagaaaac aataataaga aagccattta tttagcagtt
    14401 atagatgcag ttttacatag caataacagt aaatttgatg agttaaagaa ttacataaat
    14461 aaaagtgtat atgaagagaa cttaaggtat gaacaagaga ttattgatga atttagcttg
    14521 attaataaaa cgaaagttca attcaatgct atggattaaa taggaggaga atcatggtaa
    14581 attatacaca agaaatatat agaaagatgc tagatagtgc ttcatttcct gggaaattaa
    14641 ataatataga taatataaat atagaaaata atacaaaact attgaatgga gcaattggta
    14701 tagcaatcac tttattagac caagaagtta ccttctatat agaaaattat actcaaaaag
    14761 atataaaaaa tataagagca cttacaatgt caaatcaaat agattataga aaatcagatt
    14821 atatattttt agatattaat tcagaagtgg atatattaca gattaaaata ggttcttttg
    14881 aatatcctga tgaaagtgca actatagtac atcaagtaaa tgatttatca acgtactctc
    14941 aaactaagta tataaagctt gatttgagtg gtcctggtat aaaatctaaa aatagtatat
    15001 atataaatgg aattagagaa gaatttatag aaagattatc tagcataaat aaagattatc
    15061 caataggaat agatttgatt ttagtagata aaaaaggaga aattgcattt ataccaaggt
    15121 catcaaaatt aagttgggag gtattataga aatggcttat gtagcagtta aaggtggaga
    15181 agaagccata ttacaggcta aaaatatact tgagttttat agggtaaaag gagatagctt
    15241 gcctatagat gtaaagcaga ttgaggaaca aatgtcttat gctgtagaca aagtaatgtc
    15301 tgaaggaagt ttatacaaca aggaactatc agcattggct ataaaacaag ctcaaggtga
    15361 tatattagaa gcctctttct atgtaagagc tttaaggtca acaatgccta gaattacata
    15421 ttcagaaccc ataaatacag aagaaatgag aataaaaaga agaatatcag ctacttttaa
    15481 agatatacca ggaggtcagt atcttggggc aactagagac tatgaaaata gaatattgaa
    15541 tgtagaatta ttagaagaag atgaaaaaaa gaatagacat tttagagata gttttataaa
    15601 tgaattagaa acagtagatg aaatccctga atttacaaaa atatctaata tatttagaaa
    15661 agaaaattta ttacaagaag aagtttgtga aaacaaattg gaagaagtta ttgatgatat
    15721 tacaagaaat agtttaagat ttccagtttc taggtcagca aagttacagg cattaactag
    15781 aggtgaaagt ggagtagtgt caggttttgc ttatgctatt gtaagaggtt ttggtgatga
    15841 acatccatat ataagtgaac ttagaactgg taatgtattt ataaaagtaa aacatcccat
    15901 ctatgaagat gaagaaataa taatagggga tatgctggtt actgaatgtg aaatagtttc
    15961 aaaagcatca caaaataaga ctgaaaattc taaaactaat tcggaagata aaaataagat
    16021 gtctttagga tatggtcttt gtatggggaa taatgagaat aaggctatat ctatggccat
    16081 actagacaaa tctttggagc caaatagtga aaaactagca caagatgaag aatttgtatt
    16141 gcttcatata gatggaatag attcattagg atttatatct cactttaagc ttcctcacta
    16201 tgtagatttt aaagcggatg tagaaagaat aaaatagttt gaggaggtaa gtttaggtga
    16261 tatataacta tggtttttta gacgaaaaca caaaaaaaga aataagaaga aagatattaa
    16321 aagcaatatc aatacctggt tatcaagttc cctttggttc aagagagttg cctatagcta
    16381 agggatgggg tactggaggt ttacaattaa cactatcatt aattggtaaa aatgatgtag
    16441 taaaagtaat tgaccaagga agtgatgatt ctacaaatgc ttgtaatata aggagcttta
    16501 ttgcaagtgt aagtaatgta gaaaccacaa aagatacatt agaagctaca ttgattcaaa
    16561 cgagacatag aataccagaa gaaaaactta aatctaatca aatactaata tttcaagtac
    16621 caataccaga aacccttaga atagtagaac ctagtgaagt agaaacaaga agaatgcatt
    16681 cagaagaaga ttatagtagg atgtgggtat atctttatga agatatagtt agatttaatg
    16741 atatatctat agcagttgaa tatccatgta aagtaaatga tagatattta atgaatccat
    16801 cacctatacc tagatttgat ataaaaaaat taaatatgtc agacaattta ttcttgtttg
    16861 gtgcaggtag agaaaaaaga atttatgcta ttcctccata tacaaaggta gaacctttag
    16921 agtttgagga ttataagttt gaagaggaaa agtttgaagg aaaatactgt agtttatgta
    16981 acagtacaaa tacattttta gatgaagtat atgacagtga cactaatgaa aagtactata
    17041 gttgctcaga tacaagttat tgtgaaaaag ttagattaaa aaataacagt atagatgtga
    17101 caataggagg tacttggaat gagtaaaata aatgactcta aaagattaga aaatatagac
    17161 aagcaagtat taataggaag aaatatcaat cttatttatg gagatgggtg tgaaaagtgt
    17221 tttgaaagca caggatatga aagcaataat atatgtaagt attgtggttc tatagttgca
    17281 tgtaataatg taaatttaga actttatgaa ggagaagtcc ttggaattgt aggtgaatct
    17341 ggttcaggaa aaagcacttt attaaaaata ctgtttttcc aagaacatgc aaatagtgga
    17401 gaagtatata tatcccatta caataaagac acaaatatac tagagctttc agaccaaaaa
    17461 aagagataca taaagaatca ttttatgggt attgtgtatc aaaatcccca ccttggttta
    17521 aatttagact ttagtagtgg tggtaatata gcagaaaaat tattgatggc aaatttatat
    17581 aatgttgaaa aaataagaga tagaggcaaa gaacttttaa taaaaactaa tatacctgta
    17641 gaaagaatag accataaacc aaaatacttt agtgggggaa tgcaacaacg agttcaaata
    17701 gcaaaagctc tttcaaataa ccctccaata ttgctattag atgaggttac aacaggttta
    17761 gatgtttctg tgcaagcgga agtattggat ttaataagag aaatccaaag agaacttaag
    17821 atttctatga tagttgtatc ccatgacttt gatgttataa agatgcttgc tgataggaca
    17881 attgtaatga aaaatggaag tgtaattgag tctggtctta cagaccaaat aatggaggac
    17941 ccacaacacc catatactca gcaacttata aattcattgc tataaatatc gtttgtaaat
    18001 aagcaaaatg ggggttttta ataaaatgaa agaaactata cttgaagtaa ataattttag
    18061 taaagacttt gagttacatg cattgaacaa gactataacg gcatgtagta atataagttt
    18121 tgaagtttca aaaggtgagt ttttaggaat aataggtaaa tctggagcag gtaagtctac
    18181 catattaaaa agtatctata aaacatatat accaacaact ggagaaataa tttttaattc
    18241 agaaatatat ggtaatgtag acctggctaa aattggagac cgagaaatga taaatcttag
    18301 aaagaaagaa ataggatatg tatcacagtt tttaaagaca cttccacgaa taactgcaat
    18361 tgaactggta gtacattctt taattgagag tgggtttgaa aaagattgtt cgtatgatat
    18421 ggcaaaagat atacttaacc aatttgaaat aaaggaaaat ttatgggatg cgtatccaaa
    18481 taatttttct ggtggagaaa aattaagatt aaatttagct caagctatgg ttaaaaagcc
    18541 tagacttttg ttacttgatg aaccaactgc atcacttgat aatcaatcta aaatatatgt
    18601 aaaagaaaaa atgttggagc ttaaaagtca aggaactaca atgataggaa tttttcatga
    18661 tatagagttt atggaaactg ttatagataa gacctttact atgacaaaag ggactataag
    18721 tgaaagtggg gtagcctaga tgataaaact aattcataat gcaaatattg tattagaaga
    18781 tatgatttta gaaaatggtc atttaatcat agagggagat acaataaaaa aagtatcaaa
    18841 tgataagatt gattacgcat ttgactttaa tgagatagat gaaataatag atgcaaaaaa
    18901 cttatatgta atacctggta ttatagatat tcacagtgat gccattgaaa aggaaataga
    18961 gccgagacca agtacattat taccatttaa tatggcattt tatgaactag ataaaaaact
    19021 acctgcaaat ggaataacta cagtttatca ttctatatct ttaggggatg gagtaggagt
    19081 tagaagtatt gataactctt taaagatgat taaaaatatt gattcatata aaaatataga
    19141 tagtaagagc ataaatcata aagttcattt aagatatgaa gtgttgtatt atgaaggttt
    19201 agaaaaggtt ttagaactat tagatgaaaa taaaatagac tacttatcaa taatggacca
    19261 ttctccagga caaggtcagt atacaaatcc aacattttat aaagagtatg ctactaaagt
    19321 gtggggagtt acagaaaatt atgtagatac atggttagat gatttagtaa atctgcatga
    19381 taatttagat tggaataaaa tagctaatat tataggtata gccaaaacta aaaatataaa
    19441 tgttgcatca catgatgatg atacattaga aaaaatggat ttcataaaaa atataggcat
    19501 aaatgtatct gaatttccta taactcttga agttgcaaaa cattcaaaga aattgggtgt
    19561 tctaacttgt ttaggtgcac cgaatattgt aagaggtaaa tctcataata acaatttaaa
    19621 agcaatggat gctatattgg aagattgttg tgacattgtg tgttcggatt atcttccatc
    19681 agctatgata aagtctatgt gtatagttgc agaaagaata aataatttaa ataaagcagt
    19741 cagtttattt acctcaaatc ctgctaaagc agttggtata tatgatgaaa gaggtagtat
    19801 aaaagaaaat aaaaaagcag atttagtttt agtagatatt gatagtgaat atccaaaggt
    19861 tgtaaatact attgtaaatg gaaaaacagt ttataaaagg gaggtataaa gattgctaac
    19921 agagttacat tgtcatacaa gtatatcaga taataatttt actactgaag aaataataaa
    19981 aaaagcagca gaaaagaata taaatatctt agcaataaca aatcatgaca cattatctgg
    20041 attggatgaa gctcttgtgc ttggagagca ttatggaata acaataatac caggtataga
    20101 aatttcagcc tatgattata aaaatagaaa aagagttcat attcttggat ataacataga
    20161 tttaaacagt caagaaataa aaaatttgtg caatccaatg gtatgtgaca gacataaagc
    20221 atctgttaag atggtaaata aaattataga tttaggatac aaaatatctc tggaagatgt
    20281 aaagaaatat tcatcaaaga ctggaatatt taaacaacat ataatgaaag ctcttataga
    20341 taaaggatat acagataata tatattcaaa tctatataaa gaattgtttc atagaggaaa
    20401 cggcaaggta tatgtatctt taaaatatgt ggattacaga gatgcaataa aagctataaa
    20461 aaattctggt ggaatttgtg tgttagctca cccgggtcaa atggataatt ttagcgctat
    20521 tgaagagatg gtaaaagtag gattagatgg aatagaagta tatcatccat ctcacaacaa
    20581 agaactagaa aaagagtcgc tttcttatgc taagaagtat aatctagtgg tcacaggagg
    20641 ttcagattac cacggatttt attctcaaca taattatgaa cttggaagca agagcttaaa
    20701 taaagaagat ttatttaagt ttaggttagc tttaggaggg gtattataaa tgtctttttt
    20761 acaagtaaaa aatgtaggta aaagttatgg acaagttaag gtgttaaaag acatatctat
    20821 tgatattgaa aaaggagagt ttatttgttt actaggtcca agtggttgtg gaaagagtac
    20881 actacttaga ataattgcag ggcttgagga taaacatggt ggaaaaataa taataaatga
    20941 taaggatatg actaattcac cacctgaaag tagaaatttt ggtatagttt ttcaatcata
    21001 tgcattattt ccaaatatga atgtttacaa aaacatagcc tttggactag aaaataaaaa
    21061 cataagtaaa tctaatattg ataaaaaagt taaagaagtt ctagaagttg tagaattaag
    21121 tggatatgag aaaaaatatc catcacaatt atcaggtgga caacaacaaa gggttgcatt
    21181 agctcgtgcg attgctttag agccagactt tttacttctt gatgaacctt tatctgcatt
    21241 agatgcaaaa gtaagactta aacttaggga gcagattaga agtttacata gaaaactagg
    21301 cattacaact ataatggtaa cgcatgacca agaagaagct ctttgtttag ctgataagat
    21361 ggttgttatg aatagaggag aaataataca agttggaacg ccaaaggagg tatataaaaa
    21421 tccagaaaca ccttttgtag ctgattttat aggaacaata aattttatag atgatggtat
    21481 taataagata gctattagac cagaagatat aaaagtagag tctaatagag attctaaaga
    21541 taaggatata aaagtgggag aaattttaga tatcgaattt agaggattta actatagaat
    21601 tacagttgag tatcgttcta agcaaatgaa attagatgta gtttccaaag ttgcagaaca
    21661 aatgaaactg tgcataggtt ctaaaataaa ctttaagata cctaaagaag gaattgtaca
    21721 atataaatca gagggatgtg cttaatagct aggggtgaat aaatgataac atcaaaaaat
    21781 aaaagaagaa taagtgatag cacaatacaa aaatatttct tgattttttc ggtaattgta
    21841 ctaatagtat ttatattatt tccattagta tcattattaa aaaatgcatt tatggataca
    21901 agtggaaatt ttataggaat ttcaaacttt agtaaatatg ttgaaaatcc aagtcttata
    21961 gcatcattta aaaattcaat ttttgtatct acagtctcat ctttaatatc actatgttta
    22021 gcatttatat attcatacgc aataacaaga acaaacataa agtttaaaga tttttttaaa
    22081 actatgggaa tgtttccact ttttgcacca actatgttat atggtatttc acttatatac
    22141 ctttttggaa ataagggaat ctttacaacc atgggtctgg aagtaccact atatggtcca
    22201 ttaggaataa ttatttctca gattctattt acatttccac aagctttttt aatattatct
    22261 gtagcacttt cgatggctga ttatagactt tatgaagcag cagatagctt gggtgctagt
    22321 gaatttaaga aattttgcaa aataacaatt cctggtataa agtactcatt acttagttca
    22381 ttctttgtat cctttatact tgcatttaca gattttggag cagctaaggt agtcggagga
    22441 aactacaatg tactggctac tgacatatat aaacaagtag ttggacaatt taacatacca
    22501 atgggagcta ctgtttctat ggtaatgtta attcctgtac taatagcatt tacattagat
    22561 aagatggcaa gtaagaaaca aggtatgacc ataactgcaa agtctatgcc atataagatt
    22621 aaggaaaata agtttagaga tagagtattt ttaatagtat gtagttttat aacattgcta
    22681 atttttattt taatattcgt ttcagttata ggttcagtta taaagttatg gccatataat
    22741 ttaagcttta caatggaaca ctatacattt aaaggtattt tgggaagtgg aataaaaaca
    22801 tacttaagca gtttgaaaat atcaacttta acagcaatat ttggaactat atttgtattt
    22861 tttagtgctt atatgataga aaaggtggat aagtttccta aattaagaca aacagcttac
    22921 cttttatcaa tgataccaat ggccttacct ggcttagttt taggtatatc atatataact
    22981 ttttttaact cagcaagtaa tccattaaat tttttatatg gaagtacagc aatattagtt
    23041 atagtaaatg tagttcattt ttattcagtt tgttttataa cctcaaattc atctttaaaa
    23101 atgttggata aagagtatga attagttgca aaatccataa atattccatt ctataaagta
    23161 ttttttaata taacagttcc aatgtctata acatcaatac ttgaaatagt agtatattat
    23221 tttgtaaatt caatggttac tgtttcagca ttaatatttt tatatacacc tcaaactcag
    23281 actgcatcaa tatcaatact aaagttagat gagataggat atataggaac atcagctgct
    23341 atggcagtat tagtgctttt aacaaatata atagtaagac ttttgtacga atttgtaaca
    23401 aagaaattaa aaaataacac acaaaaatgg cagcaaaaag atgttgcata ggggaggtaa
    23461 aaatgaaatt taagaagcta gtttctttag gagtattatc aacactttta ttaagtgtaa
    23521 taacaggatg tagtactgat tcatcaggtt cagattcaaa gtctgatagt aagacaaaag
    23581 gggatttaac agtttacaca gcaattgaag aagatagtat agagccatat ttagctacat
    23641 ttaaagagaa atatccagat attaaattaa atattgttag ggcatcaaca ggagatataa
    23701 cagcaagatt attagcagaa aaggataatc ctcaagcaga tgttgtatgg ggagttgcag
    23761 ccacaagctt attggttgca gatgaccagg ggatgttaga accatatgct ccaaaaggtt
    23821 ctgaagaaat agagtctgca tttaaagatg ataaagaagt tccttcttgg gttgggatag
    23881 atgcttggat gacaggtata acagtaaaca ctaaagaatt aagtgataag aacatcccag
    23941 ttcctagttc ttacgaagat ttaataaagc cagaatataa aggtcttata tctatgccta
    24001 atccatcttc ttctggtaca gggtacttaa cagtatcagc ccttatacaa attatgggtg
    24061 aagaaaaagc atggcagtat atggataaat tgcatgagaa tattggtgta tacactcaat
    24121 ctggttcagc tccagctaca tcagcagctt caggagaata tcctattggt atatcttttg
    24181 gatatagagg tataaagtta aaagaggaag gctatccagt agaagtagta tttccaaaag
    24241 aaggttctgg atgggatata gaagcaaatg ctcttgtgaa aaaagataat ataaaagaag
    24301 catcaaaagt atttttagat tgggctataa gtaaagatgt aatgaacgaa tactctaaga
    24361 actatgcagt tactactatt tctacaggaa atccaatacc agaaggattc cctaaaaagc
    24421 cattagagca aatgatagat aatgatttaa aatcagcagc aaaaaataga gaagatatac
    24481 taaataaatg gatatcaaaa tatgatggta agacagaaaa agagagttaa gtaaaactat
    24541 acaaatggag taaaaagaaa gaattttttt aagtagattt tattataata atataagttg
    24601 agaataatat aggtcaagaa aagtgaatat tctataaaaa tgtatagagt aagtatacca
    24661 ttcagtttga atggtatatt ttttattttt agagatta
//
LOCUS       DACULQ010000044        24355 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.044, whole genome shotgun sequence.
ACCESSION   DACULQ010000044 DACULQ010000000
VERSION     DACULQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.044"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            99..515
                     /locus_tag="JA154_07090"
     CDS             99..515
                     /locus_tag="JA154_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995629.1"
                     /translation="MYKEKLFDNYFKFLALLFWPIMWYKWIVISNGTLENMLFTIYAI
                     VAIVFIILYSVFMIKYKDITQIDFFYRISTLLAFIFTLFSFLIYPKSLFFLYLKIIFT
                     GIYLYYSIVKTLKFKDDEGVVGIMSSLLLIVITLFY"
     gene            636..1391
                     /locus_tag="JA154_07095"
     CDS             636..1391
                     /locus_tag="JA154_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAT7995630.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(1534..1962)
                     /locus_tag="JA154_07100"
     CDS             complement(1534..1962)
                     /locus_tag="JA154_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAT7995631.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(2383..2583)
                     /locus_tag="JA154_07105"
     CDS             complement(2383..2583)
                     /locus_tag="JA154_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAT7995632.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(2766..3920)
                     /locus_tag="JA154_07110"
     CDS             complement(2766..3920)
                     /locus_tag="JA154_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HAT7995633.1"
                     /translation="MKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNMEEKVM
                     ESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYENEVKK
                     MKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQKDKENA
                     FRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGVELQLT
                     DEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGRFKKNS
                     IWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFVGYEKK
                     GRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            4171..4992
                     /locus_tag="JA154_07115"
     CDS             4171..4992
                     /locus_tag="JA154_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine kinase"
                     /protein_id="HAT7995634.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRHEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(5068..6456)
                     /locus_tag="JA154_07120"
     CDS             complement(5068..6456)
                     /locus_tag="JA154_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7995635.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGSLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVRNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(6591..7349)
                     /locus_tag="JA154_07125"
     CDS             complement(6591..7349)
                     /locus_tag="JA154_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HAT7995636.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFASSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(7351..8067)
                     /locus_tag="JA154_07130"
     CDS             complement(7351..8067)
                     /locus_tag="JA154_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HAT7995637.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(8069..8776)
                     /locus_tag="JA154_07135"
     CDS             complement(8069..8776)
                     /locus_tag="JA154_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HAT7995638.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEKLFMKVVAENRKRGQ"
     gene            complement(8892..9428)
                     /locus_tag="JA154_07140"
     CDS             complement(8892..9428)
                     /locus_tag="JA154_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAT7995639.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(9530..10600)
                     /locus_tag="JA154_07145"
     CDS             complement(9530..10600)
                     /locus_tag="JA154_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995640.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(10593..11651)
                     /locus_tag="JA154_07150"
     CDS             complement(10593..11651)
                     /locus_tag="JA154_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995641.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(11638..11970)
                     /locus_tag="JA154_07155"
     CDS             complement(11638..11970)
                     /locus_tag="JA154_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAT7995642.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            12169..13290
                     /locus_tag="JA154_07160"
     CDS             12169..13290
                     /locus_tag="JA154_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HAT7995643.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(13418..15316)
                     /locus_tag="JA154_07165"
     CDS             complement(13418..15316)
                     /locus_tag="JA154_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAT7995644.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            15631..16407
                     /locus_tag="JA154_07170"
     CDS             15631..16407
                     /locus_tag="JA154_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087838.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAT7995645.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(16499..17251)
                     /locus_tag="JA154_07175"
     CDS             complement(16499..17251)
                     /locus_tag="JA154_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAT7995646.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(17412..18362)
                     /locus_tag="JA154_07180"
     CDS             complement(17412..18362)
                     /locus_tag="JA154_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAT7995647.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            18747..19943
                     /locus_tag="JA154_07185"
     CDS             18747..19943
                     /locus_tag="JA154_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087835.1"
                     /note="catalyzes the formation of oxalozcetate and
                     L-glutamate from L-aspartate and 2-oxoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAT7995648.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(20055..21395)
                     /locus_tag="JA154_07190"
     CDS             complement(20055..21395)
                     /locus_tag="JA154_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HAT7995649.1"
                     /translation="MKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVVEE
                     RQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKIPL
                     QFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALKRV
                     FPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDLVP
                     KIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKVAK
                     SNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAHNK
                     HQIAIVITENNGAISNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAYEK
                     LAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(21534..22286)
                     /locus_tag="JA154_07195"
     CDS             complement(21534..22286)
                     /locus_tag="JA154_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT7995650.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(22434..24011)
                     /locus_tag="JA154_07200"
     CDS             complement(22434..24011)
                     /locus_tag="JA154_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAT7995651.1"
                     /translation="MKQKMKGFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     MPFLANNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGIIAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYAIVYYNVFKILILRFNLQTPGREEITDINFDLKPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKDNIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
BASE COUNT         8326 a         3912 c         2750 g         9367 t
ORIGIN      
        1 gtttctataa attcaatatt aatatataat aatgatatca tattatttaa aattctaaaa
       61 acaaatatat gcttttgtta agaaaggttg tttgatgaat gtataaagaa aagttgtttg
      121 ataattattt taagttctta gcactattat tttggccaat tatgtggtat aagtggatag
      181 taatttcaaa tggtactctt gaaaatatgt tatttaccat atatgcaatc gtcgcaattg
      241 ttttcatcat tttatattcg gtgtttatga taaaatataa agacattaca caaatagatt
      301 tcttttacag gataagtact ctacttgctt ttatatttac actttttagc tttttaatat
      361 acccaaagag tttatttttc ttgtatttaa agattatttt tactggaata tacctttatt
      421 attcaatagt aaaaactctt aaatttaaag atgacgaagg tgtagtgggt attatgagct
      481 cacttttatt aattgtaata actttgttct actaaattta attctatttg acttaatatg
      541 gctttaaata ttagacatct acattatttt ctgatattat ttaagatggt agtcttatat
      601 taataatgaa atacactatg aaggagttaa ctattatgga aaaaaaagtt ttagcaacag
      661 ttggagaaaa agagataact aatattgata ttgaaaatgc attaaaatca ttagaccctt
      721 atcaagctat gcaatttaaa actgaagaag gaaaaaaaca cttattaaat gatttagtga
      781 atcaagaatt attcttctta gatgctaaag aagaaaaatt agatgaagaa gaaatattta
      841 aattagaaat gaaaaagata gaagaaaatg tattaaagca atttgctata aataaagttt
      901 tatctagtgt taatgtaaca gaagatgaaa aagtaaaatt ctttgaagct aataagagct
      961 ctttctctaa accagaaagt gcaactgcta aacatatatt agtagatagt gatgaaaaag
     1021 ctaaagaaat acttgctcaa ataaaatcag aagaaatatc ttttgaagat gcagctctta
     1081 aacattcttc ttgcccatct aaagatatgg gtggtgattt aggaacattc ggtagaggac
     1141 aaatggttcc tgaatttgaa gaagctgtgt tctctatggc taaaggtgaa gtaagtgaac
     1201 cagttaaaac tcaatttgga taccatataa taaaattaga agaccttcaa gaaagtacag
     1261 agtctacatt tgatgaagtt aaggctgaag ttgaaaaaag tttattatat caaaaacaaa
     1321 atgaagttta tggaaataag ataaatgctc ttaatgctaa gtacggaaac cttgtaaaat
     1381 acaacgatta atcataaggt catatataaa aaagctgtct tttcacaata tttattagaa
     1441 aagacagctt tttatattaa agtttaaata ttcagtttca tattatcatt cttttattgt
     1501 aaagaactca gcttaaaagt ttaaataaga tttttaatta tcattagcta aatttttata
     1561 tccacgtatt atctcataac agtaacctat tacatctctt ctttttatta tcccaataaa
     1621 tgtgtcttgg tcatctataa caggaatgaa attttgattt aaagatttta taactaaatc
     1681 ttcaatatct gcgttgatgg aaacaggaga attatccatt tttctattta tatctgtcac
     1741 tggaacatct tccaatccct ttaagtccag atttaaatca ttttttaatg tccataaaaa
     1801 atcaccttct gttatagttc caacatattt accatcctta cttattattg gaattgcaga
     1861 gtatttgtga tattccattt tttctagggc ttgccttata gtgtagtctt catatatata
     1921 tgctacttca ctttttggtg ttagaaagaa taatatgttc attacactac ccaccgttac
     1981 tgaaaactta taaatattac tcaatattta taatgttact tttttgcagt aactttctcc
     2041 tttcttattt tctaagtatt cttaatacta tacgctgtaa caagcgtttt tcctttgctc
     2101 attttaatct tattatgctt tattaatatt tttatatggt ctatcagtat cacttattct
     2161 ctttttagaa aatttactaa aaattaattg acttaaactt agccataata attttactct
     2221 acactattaa ttttaacata tttatctcat tattaatgat tttattttaa taattttaat
     2281 ttattttgct attaatttta tttacaaaaa ctgaaaaaac cgctaaatga ttagcggctt
     2341 atctctcata ttactagaac actaatatat atgaattttt aattataatc taactacgtt
     2401 agcagcttga ggacctttag ctccatctac tacttcgaac tcaacgcttt gtccttcttc
     2461 taatgttttg tatccatctc cagttatagc tgagaaatgt acgaaaacat ctccttcacc
     2521 ttctacagat atgaatccaa atcctttttc gttgttaaac catttaacta caccagtttt
     2581 cattaataaa tcctcctaaa aaaacacaaa tatttagcta ctcttttctg taaccttatt
     2641 actactctat catatatagc tatgtttgtc aattattatt atttttatac attaataatc
     2701 aattttttta ctttataatt ttaaatgatt attatctttt acactttatt tttctttata
     2761 attttttaat aatttatttt tattatatgg aatcttatgg acttatcact ccaacctaaa
     2821 tcccatggca ccaacattct atcagtatta tgacaagtta ccaaaggata ccctcttgag
     2881 tctaatcctg tcactgtcga aacatgtgtt attctgcctt tcttctcata accaacaaaa
     2941 tcccctggac gtaaattata agcttctttg tatacttctt cataggaacc tttagcaatt
     3001 aatgaacctc ttccactata cagtgcataa tttttaaaag cttgtgcatt tacccatgct
     3061 tttgtacctg ctctctcgct ataattccaa attgagttct ttttgaacct tccactttca
     3121 aacattatct gtgatgcaaa gtttgcacaa tctccacctt ctggattata atccctatag
     3181 tttggattaa atttcatacc atgttcttca tcagctgcgg ctccacagta tttatgtgca
     3241 taatctatag cctttttttg ttcatctgta agctgtaact ctacaccatc ttgttgacca
     3301 atataatttc ttatatcatc agattttata ttttctaaat ttaaagagtc tgcaaaaggg
     3361 tctgtatacc actctttagt tatgacatat tgattatcta taatttttac atttagataa
     3421 tgatatgtac ctattctaaa ggcattttct ttgtcctttt gattttcata tgaatactta
     3481 tattcagtcg atactgaaca gattatacca tagaggtctt tttctttttc ttttattttt
     3541 ttaatcttaa cccttgtctt tatgtcttta aattttacac cttgcttact agaccaattt
     3601 tctaaatact tcatcttttt tacttcattt tcataagccc acagaccaaa ttttttatcc
     3661 gtctcataaa gaccttttaa aatgttttca tttttctcta atattgcctt atttctgtaa
     3721 tcaaataagt tttcaagcaa tagtttatat tggtctttta catcatcacc ttcaacattt
     3781 gcagattcca tgactttttc ttccatattt ttcatactta aatcattgat taaaaataca
     3841 cctgctgtag tagctaaaga caaaaataca aatgccatta tacctttgat gtacttattc
     3901 ttatttttca gtattttcaa agctacacct cccaaatagt acaccttaca aatcaaatta
     3961 caaagaatat tctgtcacat atagtaattt tcatatttaa taattctcta ttttattatg
     4021 tccaatatta aaaattaatt gtatcaatac actgtttttt ttacaatttt ttattttata
     4081 atctactttt ttcttttttt tatagtaata tatatgtata taaatatttt ggtatcatta
     4141 taattaatac atactaatgg agggtttttt atgcaaaaaa aagtcgcagc aattaatgac
     4201 ttatctggaa taggaaaatg ctcattatcg gttgctatac ctatactttc agcattaaag
     4261 gttcaatgtt gtccatttcc aacggccata ctttcaagtc aaactggtta tcctgaattt
     4321 acttttttag actttaccga tgaaatggta aaatattcaa atgtttggaa aaatttaaaa
     4381 gtcaattttg atagtatata tagtggtttt ttgggttcaa agcatcaaat tgagatagtg
     4441 gctaatttta taaacgatta tcccaatgct tttatcgtag ttgaccctgt gatgggtgat
     4501 aatggagtta tgtatccaat attcacagaa gaaatgagac atgaaattaa agaattagtt
     4561 aaacattcag atttaactac tcctaattta acagaagctt gtttcttaac tggaaatgat
     4621 tataccaaaa gcgattataa tagagatgag cttatatata ttgcaaaatc tgtatctgat
     4681 ttgggaccta gcaaagtcgt tattactggt atattagaag atgataatat attaaatttg
     4741 gcttacgata gagataatga ccatgtattc tttacatcag tcaaatataa taattgttct
     4801 tatagtggaa ctggagatat attcacatct atactttgtg gtatgttagt aaataaacat
     4861 gatttgggag tagcggttaa tactgcaaca gattttatat ataaaactat aaattacaca
     4921 tctcaatttg atactgatag aaatgatgga gttatgtttg aaaacttttt atcagattta
     4981 actaatatat aaagtcaaaa aaactatcca ttttataaat acctataaat cataaaattt
     5041 aggtatttac aaaatggata ttatttacta aactattggt atttcaattg ttaccatagc
     5101 accaccagtt ttcacagaat tttcaatata taaaatccca ttatgttttt gtacaatatt
     5161 atttacaata tagagtccca ttccataatg agatttagaa tttctactca aatctcctgt
     5221 ataaaactgt tctgtagctt ttgacaaatc tgctttagaa aaaccacttc cacaatctgt
     5281 aactacaaat tttaagtatt tattacgtac tgatacactt atgtatagtt tactctggct
     5341 tggtgaataa tctatagcat ttgaaattat attcataata gctctgaata acagttctct
     5401 atctatagtt atatttttag gcaaattatc ttgtttataa aactccacat ctaagttttt
     5461 agtactagct aatgcttcta tttgcactaa gacatctttt ataaactcac tcatactgat
     5521 tgcttcttta tttaatatat agccattctc tgtttttgat atatctatta aagtattaat
     5581 atatttttct atttgagtga caccctttga gatatattcc atgtattcta ataactcagg
     5641 attttcattt atttcaatta ataagtcagt atttccatgt ataattgtta gtggtgtttt
     5701 tatatcatgt gccagtgcag atatctgttc ctttctattt tcttctagca tccattgttt
     5761 ttctaaagaa tcttttaacg ccaacttcat tttatccata gaaaaaagga cattattaat
     5821 ttcataaatt ttactagatt ctacaacaaa atctaaatcc tgttgttcta ttttttctgt
     5881 agtatttttt aaaagctcca tttcaatatt aaatttcttg ccaaatactt ttgataatat
     5941 aacaatttca ataaaaaata ttatactaaa aataatcata cccaatgtct ctggttttgg
     6001 taaatatgct cttaaaattg gagaactata ctctgatact attttgtatt ttattataaa
     6061 aacttcatct ttcttaaaaa actttatata actttctgaa tatgcatgcc tttcttcaat
     6121 atctctcatt aaattccata cttcttttga ttctttttta ttaaaggtcc ctgaaattaa
     6181 actcccatcc aaagtataaa ccccatactc atatatactt ggaattatat cttcagttac
     6241 tttttcactt gaaataatct gttctttata tttattaaat tgtttttctg cataattagc
     6301 tggtaaaatt acacctgatg ataataatac agaaaatgat aacattaaaa ggagtacaat
     6361 tgaaattgtc ataataaaga ataaagataa atattttata aaaatagttc ttaacttttc
     6421 aacttttttg ttgttagatt ttttacctcc aagcatttgt accctgtcct ccaatagttt
     6481 aattaattca taattaatat ctaaccagtt ttttatttga ttattttata tctttttatt
     6541 gctaagttat attttaacat aataatataa ataaacagtc aatttttact ctattcttct
     6601 gattttttac cttcccattt agaaaaccaa atacataaaa ttacaaatgc taaaattgtt
     6661 acaaaaccac acattaaacc tgttgattga tatgctttta ttgattcaac ctttatatat
     6721 tcaaccgttt tagaactcga aaaagacgac caaatagtta taaatctaac accccatgca
     6781 caaggaaaat actgccaaat accagaaccc aatccagtta ataataatgc tgaaagtaac
     6841 atttcaacta tacctactcc aattgatgtt ccttttccga accttaaact taagaataaa
     6901 tgtaatatgt attcaaataa attacaccca attaaaatta gaattgaaat catataaaag
     6961 cttgatgcaa agtaagcctt agatatacca aatttaaaac ctaatacaga cactaatgtt
     7021 gctacaaaac caagacatac taagattata tatttactta taagagtaag atattttgga
     7081 ttagatgttg ttaacatatg tttataattc cctgcataat attcttgttc aacaaacatt
     7141 gaacacacaa cacctgacaa taaaggaaaa gctattgcta taacttgtaa atatgctact
     7201 atctttagta tattatcagc tcctgaaacc ttataatacc ataaaaacaa tcccatcccc
     7261 attaaagcaa tgcaaatgtg cattagtatt ataaaatttc tctttaattt tattaaatca
     7321 gaacaaaata ttcttattaa ctgtctcata ttacttcacc tctctattat tgaaccaaac
     7381 ccttgtagct aaagcaaata atacaaataa aacaatagat attaatactc ctggcaaaat
     7441 aacagcatta tttaaaaatt cacttccctc tggtacagga agaccatttg gcagaatatg
     7501 caatacaaca gccattaatc ttgaaggaat cgaatatgga aacatatacc acatttctgt
     7561 agttgagaaa acagcaactc caacaatagt aaagaacata tttattagaa ctgtaacaaa
     7621 tataccaaat tttgaagcta aaaacataca aagaggaatc tgccacataa atagtaaaat
     7681 aattaatata gttgcaatga tattttgacc tattgaaata gtttctttat agattatccc
     7741 aacaaaaact acacctattg cagaaataat tgatgctata aaacaacaaa taatacagta
     7801 aatcattttt ccagtccaaa cgtctttcat ttcaattggc aatggtaaaa taccacgata
     7861 tttacttttt tcatctttta atactattaa agaagatatt aatgttaatg ctcctggaag
     7921 aaatgtcgta taccaccagt tgaaagcagc attttgccct ccacctagtc cacataaaag
     7981 tatacaaaac actggaacta tccatatcaa cttcattcca aaagttcctt tcatcttttg
     8041 aaattccact ctaataaatc tttccatatt actgccctct ctttctattt tcagctacaa
     8101 ctttcataaa aagtttttct aagttttgtt cttttgatac ttctccttga taacccatta
     8161 ctccattaga aattattcct atttcatcta caacttgttc aacttcagac aagatatggc
     8221 ttgataaaat aactgtaatt ccttgttttg gaaaagaacg aatcaattct ctaagttctt
     8281 caataccaat agggtcaaga ccatttgtag gctcatctaa gattaaaagt tttgggtgat
     8341 ttaataatgc aatagcaata ccaaggcgtt gcttcatgcc tagagagaat tgacctgaac
     8401 gctttttaaa ggtatctctt aaatctacag tctctaaaac ttcttcaatg cgagagtcag
     8461 gaagaccaag taaagtagta tggactttta aattttctcg tgctgttaaa ttctcatata
     8521 tagcaggaga ctcaataaga gaaccaatat ttgataaatc ctttctagtc catctatgtc
     8581 cctcaaacat tatttttcct gaagttggat ttaaaatccc agttatcatt tttaataaag
     8641 tagatttccc agctccattt ggtcctaata atccatatat agaattagat ttaattgcta
     8701 gtgaaatgtt atcaactgcc atctgctcct taaatttttt agaaactcct tttacctgca
     8761 aaatataatt actcataatt ttcatccttt cttttgtcaa acttatttac aagaatcatt
     8821 ctaaatcata aatataagga ttttataaga attaaaaaac aaaaaaagat gtgttaaaaa
     8881 cacatctttt tttatcttaa ttctattaat tttccattgt agtatatctc tacttcttca
     8941 atccaaatgc tattatcttc ttcttggatg acatttttaa taatattatt tatagtatat
     9001 attatatttt gttcattcaa ataatcatct ttccaagaaa ctctactttt tttatctggt
     9061 tctatcaagt ttattttaac aagattatct ttttccttaa cttctacttc catgtctaaa
     9121 ccattaaaat actcatttga taatgtatct aagattaatc gcagtttatc cttaagtgaa
     9181 ctttcttcat caatctgtaa gtactttact attttctcat cctttgaaat tatctctata
     9241 ggtattttag tagttttttt agaattaatc attttactag ttgtttcttc ttttgcatct
     9301 tcatccttct ttacaactgc tgattcttta gaatcaacta gctcaactgc actgatgcta
     9361 taatccatac atgccgtact caataaagta gtagcaatta ttaaaaaact tactgctatt
     9421 ttattcataa gtatccctct cctaattctc tttcgatact tattatacca tagtatagat
     9481 tatattttta tacttttaaa tcaatttata aattattata gtaaattatt tacaattcta
     9541 agctatttga atttattttt atagtatatt ctttctctaa attcgacaga tttttattca
     9601 aaagcccttt tatttctctc gtatttctat acttgttgct atgttcttca ctatgttcat
     9661 aatcatatga atcattgtca tattcttcat cattattaaa ttcttcaatt gcttcttttg
     9721 actttatatc acaattaaat ttatacttat ttactggtat atcaagtctt accttagaat
     9781 ttggaacttc tatgtccagg aacttagcaa ttggcatatc agagtacaag taaaattcac
     9841 tttcataatt tgaatttcta cctattttct cttttatatt tacattttca ggtatatact
     9901 cttctatatt attgataaat acatcttcat tctctgaaga tatatacaat gactctgatt
     9961 ctatgtttac atttttaacg cctaaaattg attttgttga caagtgaaga ttgttattag
    10021 atattaagtt taggttttct aatctattta ctttgttctc tgttatagct ctactaaaac
    10081 ctccataaga agttttatat gttatagtat ttgaagctac attatcgtat acaaataagt
    10141 gtgagcttat acttgatgca tttatgtcta cattatttgg tacatatatt ataatttctc
    10201 tataatcatg tgaaaacata ctatttatag atgtatttaa taattggtcg aaatttttaa
    10261 tctttttaat tttattttca tattttcttt caccttttac aactaaagtc ttatctttta
    10321 aagttacatt gtatttgtaa aactcattat ttcctcttgt tgttacacgc acatcttcac
    10381 ttgaactttt ctttattgta acgtttatat tgtcaacagc tatatctaac ttgtcaatat
    10441 ttactttttc ttgataaagt ttattttctt ttttaaattc agcttccttt tctaaaccaa
    10501 agtttattag gcttggcata actaaaatcc ctgaccatgt tgtccctatt atacctatta
    10561 ccaataaaac cgcagcaaat atagcaagtt ttttattcat gtttatcttc tcctttctta
    10621 ttttctatat cttcaaggtc cttatatatc gcctcaaaat ttatactatc atcattatat
    10681 tcatcagttt cttttatgct ttcttcttct ctattgtagt aatcattatt atatgaatct
    10741 tcattaatgt ctttattcaa atcttcatca tttaaagtag ctttttttct tttttccatc
    10801 ttttcatttg cttttatata aatatttttt ctctttatcc aatgtatata attatttagt
    10861 aattgtttac ctagttttat ggcaaataat aaaatttgcc atgcaagtat ctcaagccct
    10921 acaaatgcta tggttgcgaa aatacaaaac atcataacat ctggaaccat tgctataaat
    10981 ttaatcacaa atggaattaa tataacaaaa gcaattaatg agcatactaa tgcaatagta
    11041 gcagatacaa tagcacatac aaacgcaaat gctggaacaa ttagaaataa agataacact
    11101 actaataacc cctttataac ttgtctggat aatcctccta caccttcttc tatacttggt
    11161 gtaagtttat catttatatt ttcttttgat ttattgtact tttccttagc ttcatctttc
    11221 aagatagaat aatccttttt tacatttgat ttaaatctac taaatttacc atttaatatg
    11281 tctgtattca ttatttttcc atctatattt tcttttgatt tactatactt ttcttttgct
    11341 tcatctttta agatagagta accttttttc atatctgatt taaatttact aaacttatca
    11401 ttcaaaacat ctgtatttat tattttttcc tcttcaatat ttttttcttt agtttgtaag
    11461 ataagttctt ttgctataga ttttggtgag ccaagtgatg ctattatctc catatcactc
    11521 tttccctcta taagaccatc tacaaaatac tcttcataat ctctcaatat atcattaact
    11581 tcgctctcag aaaaatcttt ttttagataa tcctttaaaa tttcaagaaa ctcagtttta
    11641 ttcaagttca ttacctccct catcttcact tattaaaatc tcaacagcct ttacaaattc
    11701 tctccactct tccatcagat tggataaatt ctcttttcct agtgaagtta ttctatagta
    11761 ttttctagca ggaccttcat tagattctag tatatatgtt tcgaaataca aatcttttgt
    11821 aagtcttctt agaataggat atattgttcc ttcatttaca tctattactt tagagatgtt
    11881 atgtactatc tcatatccat acatatcttt cttagatatg agagcaagta cacaaatttc
    11941 taaaacaccc tttctaaatt gtgtgttcat atactccatc ctttctttaa atacacagta
    12001 ttgtgtttta caaggtacta tgttaaaact tcttatatcc tcataataac acagtactat
    12061 gttctgcaca atagttattt ttaaatattc ttgttgatta aatccttcta taaatgtata
    12121 atactctaat cagatatatt ttatgaaagt ggtgataaat tggctaaaat gaaagatgat
    12181 aaaacaatat taatcctaac tgctcaattt ggtgctggac atataagtgc tgcaaaagca
    12241 gtcaaagaat gtattattga aaaatatagt aactataatg ttgttataca aaactttata
    12301 aatgcaagta ttcccatgat gaataagcct atggtaaaac tttatgaaaa caatacaaaa
    12361 tatactcctg gattatataa ttactattat tactttaaga aatcgtttga ttcaagacat
    12421 gatttatctc ataaattata tacacctaaa ctttctgagt atattgctga tataaatcca
    12481 gatttgatta tctctacatt tccactagct gctgcttgtg taaataactt taagataaaa
    12541 aatcctgata taaatatacc tacacttaca gttataacag atgttgtaga tagtatggaa
    12601 tgggtttttg aaaatacaga tttgtatttt gttccttctc ctgaaataaa aaatagattt
    12661 tttcagaaag gaataaatcc tgactccata aaggttactg gagttcctgt tgataagaga
    12721 tttcaaattg aaagtaaaga actatgttgt gataaatata gactattact tctaggtgga
    12781 ggaagaggtc tatttgatat agatgaggat tttatgcatt ggatagatga atttattgaa
    12841 gaacatagta attctataga aattacaata gttactggta aaaacaaaaa attatatgac
    12901 aatctaactc ataaaaaacc tctaaaaaat attaaagtac ttggatttgt aaacgacatg
    12961 tataatctaa taagagaatg tgacctaatg ctaactaagc ctggaggagc aactatattt
    13021 gaagctattc aatcacaaac tccagtactc gtaaaaatgc caaaagttgg acaagaaatt
    13081 gaaaacgcta aatttataat agataagggt cttggcatga tttatagtga tgacctagac
    13141 cttaaaaaca tattttataa attagtatca aatgaatttg attctataat aaactttatg
    13201 aaaaaaaatt taaaagaatt taaaacagtc attcatccag agaaaattgc tgattatata
    13261 tcagaattaa tagataatca ttacagttaa attgtactat taaaaaattt caaacataaa
    13321 attaaaggca atcaagtatg attttacact tgagaattta ctcatagtgt ttaataatac
    13381 ttgattgcct ttatcatatc aaaatcaaat tgttatatta gtatatattt ttcttgtact
    13441 gcatatacag ttgttgtatt tcttccatac ttctctttaa ttctatttgt aaatctgaag
    13501 cagtctggta atccttattt tctatagctt ctctcactct agtgaatagt gattctctag
    13561 ttaaagtatc cttcataata ttgaacttta aatccgatat tgagttccaa actacctcat
    13621 caagcgcatc catattttct tttgtgtgta tcttgacaat acttcttatt ttttgaatcc
    13681 cagcctctat tattctagtt tttaatttct tttgccactt atctatagct ccaatcttaa
    13741 atgattctat tatactatca gtaaatacat caccttgttt taaagatttt aattttgaag
    13801 catatatttc taagtttttc atattttcat aaactgtagc tggtggtatt ccaaatcttg
    13861 catttctttc ttccaaacta taatgttcaa atacatcatt ttcatctcta tatgctctat
    13921 ctttttctaa atagaaactt tcttcaccta catttttaga taactctttt tcaagttctt
    13981 ttgtactaag accactctta gctgctgctt ttataccatc caacattgtt tgataacatc
    14041 ctgctattac taaatacgtg ttagatagag ggcttggtga ccttaattca aatcttacag
    14101 tctttggatt tttcatatct cttattaatc caacaagcac tgacctattt cttgatggaa
    14161 tctcataact atgccctaaa gatgtaacta tacatacagg agcttcaaat ccaggaacta
    14221 atctattaaa tccatcatta gaagctgtta caattgggtt taatacttca taattgtaaa
    14281 gaagtcccat aagagcccca tatcctagtt cacttagata atcttctttt aaatcttttg
    14341 gagcaaatag attttttata cttccatcct ttaacttcgc acttactcct acatgtgtat
    14401 gtccaccact tcctgctact ccatgaattg gttttgcttt aaatgtaact tctagtccat
    14461 gtgatgtaaa tacatcctct ataacatctc ttactaataa ttcattgtct gctgcttgta
    14521 atggtgtaga aaacttccaa gaaacctcta attgctccat agcatggttt gttcttccat
    14581 ctatactaat agaactttgt attcctccga cctctttgtg agccatttca gggtttacac
    14641 ctagcttttg aagtataatt aaacttttct ctaaacaagt tcttataata ccatgagttc
    14701 ttttccagta ttgctctttt aggctttgtg atacatataa tttctctaag tcagctttat
    14761 cctcaggagt attaacccag aactcaagtt ctgtagctgc tgttaacatt atttcctcta
    14821 tatcatctac tgaatctatt cctatgttat ttattacatg tggatattct ctaaatattt
    14881 catacattga ctttttaaag tacttatctg ctttttgaag tactcctcta gaacatactt
    14941 ttttattatc atgtattaaa aatgcaggta tctttaatgt tccaacaggt agacctactt
    15001 cttcgtctat atattccata ttgtaatcta cataccaatg acaactttta tctggcatta
    15061 aatctacttt cgcattattt aatgtagcta tattataaag ctcaacactt gaaccatcag
    15121 tctgtatagc tgattccaaa aatttatcta tatcctccaa aaataattct acaggaatct
    15181 tttcatctgt tgcatttcct cctaaatcaa cccccattaa ggaaacaaat cttatatttt
    15241 tattgctttt taggatttct cttaattctt cattagtgtg tttatctttc tctattacgt
    15301 ataatagact atccattttt ccactctccc ataattttta tgtatatttt attttgttaa
    15361 atacaagata aatttcattt tccttacatt tatgaatttt tcttgcaaat tattcgtgtt
    15421 ttattcgtgt ttttatttat attattatat atcaagtata aaaagttcgc aatatttttt
    15481 attggaaaaa atattataca tacaatagtt taacattttt acaaatatgg ggtatactaa
    15541 aataaaagta tttttatttg aaaatttaat tttatttata atataatcta tagtaaaaga
    15601 aataataaaa aaatttagga gataaacttt atggaataca taattttcga tttggaattt
    15661 aaccaaggat ttgataaaaa actcaataaa actgtatcca atgaaaagtg tccttttgaa
    15721 attatacaaa taggagcaat taaattggat tctaaattca atataataga tacgtttaat
    15781 agttatgtaa aacctacaat ttataaagac atacatccct ttattagtcg tatgactaat
    15841 attaaaaatt ctgattttaa tgattcgcct actttccctg aggtctacaa caattttata
    15901 aaatttatat catcacaaga ccctatatta tgtgtttggg gagctggaga tttaaaggaa
    15961 ttatacagga atatcaacta tcacaagcta ccatcaaatt ccttacctaa atcttatatt
    16021 aatatacaac aacatgcatc taaatatttc aataatccag caggaaaaag cattggccta
    16081 caaaatgcta tttctatatt agaacttgat gaaaaaatgt cttatcacaa tgctttaaat
    16141 gatgcatatt atactgctaa agtttttatt aagatataca atccaagtat agtcccagat
    16201 atatatcttt atacaagtat aaaacctaaa actataagat attctaacaa aaaaagagtt
    16261 gattatgata agttatttga tgaatttagg aaaattttaa atcgtgaatt aactaaagac
    16321 gagaaaaaaa tcattaactt agcttataat atggggaaaa caaatcaatt tacattagaa
    16381 aatgttaagc aaagaaaaaa caaatagagt ttatttagat aaaaaatagg catgtagatt
    16441 aaaaaactag ttaactttaa atttatatct acagcccaat ttttatcata attaattttt
    16501 attttatctc taattcaact ggacaatggt ctgaacctaa tatttcagtg tgtatatccg
    16561 cacttactaa tctatcttct aattttttgg atgcacaaaa ataatctatt ctccaccctg
    16621 cattattttt tcttgcatta aatctatatg accaccaact ataaacacct tctttatcag
    16681 gattaaaata tctatatgtg tctataaacc cactgtctaa taatttagaa aacttctctc
    16741 tttcttcttt agtaaaacct gcatttttca tattattttt aggatttttt aaatctattt
    16801 ctttatgtgc aacgttcata tctccacaca caattacagg cttatgatta tctagtttgg
    16861 ttaaataatc tataaagtcg tcttcccatc tagttctata ttctagtctc tttaattcag
    16921 attgagaatt tggcgtatat actgtaacaa agtaaaaatc ttcaaactct agagttatca
    16981 ctctaccctc tttgtcatgt tcttctatat ttattccata cattactttt aaaggctctt
    17041 tttttgtaaa tatagcagta cctgaataac cctttctctc agcataattc caatattgaa
    17101 aatatcctgg taaatctaac tctatttgcc cttcttgcaa tttagtttct tgtaaacaaa
    17161 atatatctgc atctacttct ttgaaaaagt ctaaaaatcc tttcccaaca caagctctta
    17221 ttccattaac attccatgat ataaatttca tacttatact cctcatctaa acattgacac
    17281 actccccata gctaaagatg ggggcttttc aacaatttta ttgtaaattc ttaattcact
    17341 tttattttaa caatgctata ctataatttc atctaaaatt actatttact taatacctaa
    17401 aaatatatgt tttaatcaat ttttaacttt tttgttaaat cttcagggct cattgaagaa
    17461 acatacatat ttgttccaag ttcaaatcct ccaaaattat attttcttgg gattatgtgc
    17521 atatgaacat taaaatacga ttttgtttca atctttgttg gatgagtgtg aatacataaa
    17581 ttaaatggca tatatccatt gatattatat aacttcttaa acaacttttc aaatattgta
    17641 gaaagctcct gtatattatt atcatttaac tcaccaaact ctattttatc tttaaatatt
    17701 attcttactt ctccactata cttggttgca tatggtataa gtaccaaaaa agcttctcca
    17761 ttataaacaa ctcttctatc taaacttatt tcattttcta ttaaatcatc atacaaagag
    17821 cttttattgc tgtcataata ctcctttgaa accaacagtt cattcgtaat ttcaggtgga
    17881 ataaaagaca tagacaatat ttgagcatgt ggatgcatca aagatgctcc tgcctttctc
    17941 aagaagtttt tgaagatact aacatactct actttatcat ctttcgacaa atctctaaat
    18001 ctatttctat acataaaaaa aacatcttca aattcttttt gactcatgtt ataaaaacta
    18061 cgattatgtt tatcgctctc tatcattact tcatgaacac catatacatc tcttgccata
    18121 tcatcaatta taggaaattt attgtagaca gattttacaa gccatccttc ttcactttct
    18181 atttcaaata atgcatcagt tgcatgagct tcatttcctt tgcaaaatgg gcattcttca
    18241 tcatattcat tagtttcttc atcttcattt gggatatctg ctgtatctaa aggtcttttt
    18301 aacctatctg tagcaaatat aacaacatca ttggtaattg gatctattct taattctttc
    18361 ataattaaca cctcttagtt aatagccttt aatataatta tatgatattt atcgtatctg
    18421 ttaaataaat ttataatatt ttttataatt ttgcatattt attattatat attttgtcta
    18481 tagctgaaag attataacta aaaaatcact atcagtatat cagaatatta tcttactata
    18541 aattaattta tgaatacata atttagtggc ataaacatgg gtattttact ataatttgtt
    18601 aaaagtttat tttaaagcta tattaatttt ttttgatgat ttttacccat aagtatgtta
    18661 ttattatctt gtaaagatgt cttgtattca aacatacaat ttacataata caatatacgt
    18721 atttaaatta aatagggggt attaaaatga attattcaaa cagagttagt gcaatgcaag
    18781 cctctccaat cagaaaactt gttccatttg cacaagcagc aaaagataaa ggaatcaaag
    18841 tatatcactt aaacatagga caaccagaca taaaaactcc taaaggattt tttgatgctg
    18901 ttaaaaactt tgatagcgaa gtcttagaat atgctacatc agaaggtatt ccagaattat
    18961 tagaagcact tcaaaattac tataaaactt acgatatgaa ttttgaaaaa gatgaattat
    19021 tagtaactaa tggtggtagt gaagctttac ttttcactat gatggcagtt tgtgacccag
    19081 gagataatct attagttcca gaaccattct atacaaacta caatggattt ggtcaatctg
    19141 taaacgtaga agttaatgct gttacaacaa aggcagaaaa cggtttccac cttccatcta
    19201 aagaagaaat attgtctaaa gtagatgata aaactaaggc tataattctt tctaacccag
    19261 gaaatccaac tggtgcaatt tatacaaaag aagaacttaa tatcctagct gaaatagcta
    19321 aagaaaaaga tttatggata atagcagatg aagtttatag agaatttgtt tatgatgggc
    19381 ttgagtatac aagctgtggt aacttagagg gtgtacagga tagagttata ataatagaca
    19441 gtgtttctaa gagatacagt gcttgtggtg ctagaattgg ttctatcgct tgcaaaaata
    19501 aaggtcttat cgctcaaatc cttaaattat gtcaaggaag actttgtgtt cctacattag
    19561 agcaaatagg agctgttgag ttatacaaaa ctcctgtttc ttacttcaaa gaagttaatg
    19621 aagagtacaa aaaaagaaga gatgtattat acaatgaatt aatgaaggtt gaaggtgtta
    19681 tatgtaaaaa accaactggt gctttctata tagttgctaa attaccagtt gaaaatgcag
    19741 aagatttcac aatttggatg ttaaaagaat ttaacaaaga taatgaaaca gttatggttt
    19801 gccctgctga aggattctat gctactccag gtttaggacg tgatgaaata agattagctt
    19861 acatcttaaa tgaaaaagac cttcatagag ctgctacatt attaaaagaa ggtttagagc
    19921 aatatgtagc tttacaaaaa taatttttta aacttaattt atataaattt aatgatataa
    19981 aatgtcccct tttcagatat tatttgaaaa ggggacattt cttaattaat atattaatta
    20041 agacttagac ctgactattt ttcattttct ttatatagtg ttggccagta atctttattc
    20101 actttaatta aagcatctaa aacttgtctt gccttagttg gagagattat agttctattt
    20161 aatgttaaag ataaaatagc tttttcataa gattgttcaa agtatgcatc aactgctaac
    20221 ttttcataag ctagttgacc ttctaacagt cccttataaa atgttggtat attacctaca
    20281 tgtaaaggtc tcggtccatt ctttgtaaga atcgcagcta cttctaccat tgcatcatca
    20341 ggtaagttgc taatagcccc attattttct gttataacta tggctatttg gtgtttgtta
    20401 tgagctattg cttctgcaac ttcaactata aattccccat gagcatcatt atgcactaaa
    20461 ttagaaccta tagatgttcc tttttctata acagatttac attcttcaaa tacacgcttt
    20521 tctcttccat tcataacttc tcttgctcta gtacaattta tatttgattt agctactttt
    20581 ttatcaggat atagataata ctgtacatat gtattaggta agtaatctgg tacatcttcc
    20641 aacatatctt taaccattcc ataagtgtct aaccaagatt tatctctttg ttctgcatca
    20701 acaggttgga atccagagtt tgctactaaa tcttttatct taggtaccaa atcttcacca
    20761 tttttatcat acaaatgtgt aaaccatcca aagtgattta atccaaagta tacaggctca
    20821 aatgttcttg aatccatgtc taacaatctt ccataagacc ttaacaagtt aactggttgg
    20881 tcacacatat taagtaatct tttatcatct ggaaatactc tttttaatgc ttctgcaaca
    20941 atagcagcag gatttgtata atttaatatc catgctttag gagagtattt tcttacagtc
    21001 ttaaccattt ctatcatatc acctatagac cttaacccat aagcaaatcc accagctcca
    21061 caagtttctt gaccaaccag tccaaattgt agaggaatct tttcatctaa ttctcgcatc
    21121 ttaagaccac cagttctcat ttgaacaaat acaaaatcta catctataaa tgcttcatct
    21181 gggtttgtag tatattcaag tgttgcttta ggatattctt ctttaaataa tatttttcca
    21241 aattccccta ttaatctttg acgttcttct actacatcta acatagttac tttatttaga
    21301 ggaaatctat cactcatttt acataaagac tttaaaagtc ctggagtcca agtactgcct
    21361 cctccaacta tagctatatt gtatcttttt ttcattgtca tattaatcac ccctgttttt
    21421 tattaactaa cttgtaagat gattataata tataaagttt tctacatgaa tacttttagg
    21481 taatcattaa aaatagttca attttagtat ttttttcaaa attgtaattt ttttcatcta
    21541 ttgtattttt caatgtattt atgaaacata tattccataa gagaaagtgc tggcactcta
    21601 gatgtatagt taacactatt aaaattatat tgtggagaag atacaaataa ttttatatca
    21661 gctatatttg ataatgaatt gacacttata tttgtcagag aaattacttt aacttgattt
    21721 gtttttgcta tattacatgc cttaataact tgagcagtct ctccagacat agatacagca
    21781 attacaaggt catctttagt aatatttgat gcagccaaat acatgagagt tgaatcatca
    21841 tagtatttta catttacacc tatagctgaa aaacgttgag acatttcagt acatactaat
    21901 ctagaagacc caacaccaaa aaaatatata tgcttagaat tatgtatttc atttaaacac
    21961 atatctatat tctctacatt tattaaattt tgtgtatcca taacatcagt aaaaatatca
    22021 ctttgacttt tagtaaactc cttatttctt tcatttctat ttgaatatga aaaaactaaa
    22081 aagtttttaa attcactata tccagaaaag ccgagcttct gacaaaacct tactatgtat
    22141 gcagtcgaat taaaagtttc atttgctata tctttaatct ttttttgatt tattttttca
    22201 gaatttttta gaaaatagtt taaaagagat atttcatttg tagataattt tttttgattt
    22261 atatctaatt tgtctattag gttcatttat acctccataa taatgatttg tagttaaaca
    22321 actttctcat atttattata taaatataca ctatacagca tttatcttat gtttattata
    22381 ctataaaaaa gacaatacaa attataaatt agtattgcct tttaatattt actttaaata
    22441 gacattgcaa aagaaacttc gtctcttact tgagatacag atggcccata aattatttgg
    22501 atatctttat cagatacttt tataactccc attgcacctg tatatttttt aaaagcattt
    22561 tcatctactt tactcatatc tttaactact actcttaagc gagttataca gtttgttata
    22621 tcttctatat tgtccttacc acctaaatat ttaattatag ataatgctct cttagtagtt
    22681 tctccacttt gagctatttc tactttattt gataaaggtt ttaaatcaaa atttatatct
    22741 gttatttctt ctcgtcctgg agtttgtaag ttaaatctaa gtatcaaaat cttaaataca
    22801 ttataataaa ctattgcata cactaaacca agtattatag aataaatcca ctttgtattt
    22861 ggttgcataa ctccaaatac tactaaatct attatgccac cttgagtatt tcctattgct
    22921 actccaaata tatgcatcat cataaatgat atacctgata aaaatgcatg tataacaaat
    22981 aatattggtg atacaaataa gaatgaaaat tccaatggtt ccgtaattcc tgttacaaat
    23041 gttgctaaag caccagctag taatatacct ttaaccttcg acctattttc aggctttgca
    23101 gttttataca tggcatatgc agcagcagga agaccaaata ccattatagg tattttccct
    23161 tgagctaaaa atctagttgc ttctgttgaa aaactgattg tttcagggtc acttagttga
    23221 gctaagaata tgttcaatgc tccaaatact tcttgccctc ccacaactaa agaacctcct
    23281 acttcagtaa atctaaacat agcatttaat atatgatgta atccaaatgg tattaaaagt
    23341 ctttcaagaa atccaaatac aaatgttcct aaatatccta aactagctat accaatacct
    23401 acactctgaa taattgtatt tacatatggc catatatatg gtattattat ccccaaaata
    23461 gagaatacta ctgttgttat tattggaaca aacctagttc caccaaagaa tcctaaataa
    23521 tctggcagtt ttatattata gtatttatta tgtaatgatg ctgttattat accaactaca
    23581 attccaccaa atactcctaa ttctaatgtt tgtatgccta aagttacagc ttgacccatt
    23641 tctctcatca tgtcaggttc agcaataatc cccctacacc ctaacatata atttacacta
    23701 gtgtgtaata ctaaataacc aacaaaacca gaaaatgcag aaactgcttt ttcttcttta
    23761 gcaagaccaa cagatattga tatagcaaag aatattgcta agtttccaaa aagtactcct
    23821 gttatagctt ttgtaaaacc caatattcct tgaataaaat tatttgctaa aaaaggcata
    23881 tcactaacta tattaggatt tgaaaatgct gaacttattc ctaaaaatat acctgctgca
    23941 gatattaatg ctattggcaa taaaaatgat tttcctaact gtaaaaagaa ccctttcatt
    24001 ttttgtttca tttctatccc tccattttta aatttttaaa acgttttcta tgtcataatt
    24061 atatattttt attttgtttt tgaaacttta taatttgttt taaaatattt ttacataaat
    24121 agtaattttt tcaaaactgt aaattttttc aaacaaagac atagtaacgt tgtaacatat
    24181 ataaatttaa aaagagctta gaaaataaaa ttattgaata taaaatggaa aaatataaat
    24241 tacacaaaga gatatgagta cgtactaaca aatacgaaaa tgtataagaa attataaaaa
    24301 agtacatcaa gaaaatttaa gatgtacttt ttagattgta tttagactta taatt
//
LOCUS       DACULQ010000045        24001 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.045, whole genome shotgun sequence.
ACCESSION   DACULQ010000045 DACULQ010000000
VERSION     DACULQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.045"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..902
                     /locus_tag="JA154_07205"
     CDS             <1..902
                     /locus_tag="JA154_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAT7995652.1"
                     /translation="IKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLFYKGDIGLVN
                     NNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHIGCMSGKSKT
                     IAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRNRIISGLSDG
                     VVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGAKLIENIDDI
                     LNEYNIFNIIYKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYTGIEIKYVNS
                     IINELVLNELVVEMNNKTYSLNV"
     gene            935..3022
                     /locus_tag="JA154_07210"
     CDS             935..3022
                     /locus_tag="JA154_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087768.1"
                     /note="catalyzes the ATP-dependent breakage of
                     single-stranded DNA followed by passage and rejoining,
                     maintains net negative superhelicity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HAT7995653.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            3187..3972
                     /locus_tag="JA154_07215"
     CDS             3187..3972
                     /locus_tag="JA154_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAT7995654.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            4079..5344
                     /locus_tag="JA154_07220"
     CDS             4079..5344
                     /locus_tag="JA154_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT7995655.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDVEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDSHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            5375..6145
                     /locus_tag="JA154_07225"
     CDS             5375..6145
                     /locus_tag="JA154_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAT7995656.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMIFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            6254..6700
                     /locus_tag="JA154_07230"
     CDS             6254..6700
                     /locus_tag="JA154_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HAT7995657.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            6718..7911
                     /locus_tag="JA154_07235"
     CDS             6718..7911
                     /locus_tag="JA154_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HAT7995658.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLDNRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            7965..8405
                     /locus_tag="JA154_07240"
     CDS             7965..8405
                     /locus_tag="JA154_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HAT7995659.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            8688..9773
                     /locus_tag="JA154_07245"
     CDS             8688..9773
                     /locus_tag="JA154_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAT7995660.1"
                     /translation="MFMNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDV
                     VEIEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCI
                     ACNKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYN
                     LTQEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSK
                     KRIEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDN
                     EDLFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQ
                     RAITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    9916..10154
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10222..12861
                     /locus_tag="JA154_07250"
     CDS             10222..12861
                     /locus_tag="JA154_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAT7995661.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            12886..13143
                     /locus_tag="JA154_07255"
     CDS             12886..13143
                     /locus_tag="JA154_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995662.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            13153..15324
                     /locus_tag="JA154_07260"
     CDS             13153..15324
                     /locus_tag="JA154_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995663.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSCASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            15530..15955
                     /locus_tag="JA154_07265"
     CDS             15530..15955
                     /locus_tag="JA154_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAT7995664.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            16033..16320
                     /locus_tag="JA154_07270"
     CDS             16033..16320
                     /locus_tag="JA154_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAT7995665.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            16602..17060
                     /locus_tag="JA154_07275"
     CDS             16602..17060
                     /locus_tag="JA154_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAT7995666.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(17177..17914)
                     /locus_tag="JA154_07280"
     CDS             complement(17177..17914)
                     /locus_tag="JA154_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAT7995667.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            18131..19807
                     /locus_tag="JA154_07285"
     CDS             18131..19807
                     /locus_tag="JA154_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAT7995668.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            20015..20215
                     /locus_tag="JA154_07290"
     CDS             20015..20215
                     /locus_tag="JA154_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HAT7995669.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            20269..21432
                     /locus_tag="JA154_07295"
     CDS             20269..21432
                     /locus_tag="JA154_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAT7995670.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLT
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(21643..22065)
                     /locus_tag="JA154_07300"
     CDS             complement(21643..22065)
                     /locus_tag="JA154_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HAT7995671.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(22086..22922)
                     /locus_tag="JA154_07305"
     CDS             complement(22086..22922)
                     /locus_tag="JA154_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7995672.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIILDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            23220..23816
                     /locus_tag="JA154_07310"
     CDS             23220..23816
                     /locus_tag="JA154_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HAT7995673.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            23846..>24001
                     /locus_tag="JA154_07315"
     CDS             23846..>24001
                     /locus_tag="JA154_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HAT7995674.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDL"
BASE COUNT         9554 a         2587 c         4370 g         7490 t
ORIGIN      
        1 atataaaaga actactttac aaaaaagcga ttaaatatat ttgtaaatat gataaagaat
       61 atcctgaaaa tttaaagaat atatacaacg ctccaaagtt attattctac aaaggtgaca
      121 taggcttagt taataataat tttaatatag ctatagtagg ttcaagaaaa cctacagcct
      181 atggaattaa ttgtgcaaaa acaataagtt gtcaactatc ccaatatgga gttaatatag
      241 taagtggatt agctatagga atagatgcat actcgcacat aggttgtatg agtggaaaat
      301 ctaaaactat agcagtactt ggttcaggag tagacaatcc tcttccaaaa caaaatttac
      361 atttatcaaa taaaatatta gaaaatgggg gattattatt atcagagtat aatattaact
      421 cgacagtagc cccgtaccat ttttctaata gaaatagaat tatcagtggt ttaagtgatg
      481 gagtagttgt tgtagaagca gcaataaaaa gtggtgcact aatcacagtg gattttgctc
      541 ttgaacatgg aaaaaatgtt tttgcaatac ctggtaatat aaattctcaa atgagtagag
      601 gttgtcataa aattataaaa gaaggtgcaa aattaattga aaatatagat gatattttaa
      661 atgaatataa catatttaat attatttata aaaaaataaa tcaaaaatat gataatataa
      721 gtctaaatgc caagtctaag caaataattg aagctataaa aagagaagga aacttgcata
      781 tagatagtat ttgtgattat actggtatag aaataaagta tgtaaattca ataatcaatg
      841 aattagtact aaatgagtta gtagtagaaa tgaataataa aacgtacagt ttaaatgtat
      901 aaattaagat taggtagtgg gggtgtatgg ttttatggcc aaaacattag tcatcgtaga
      961 atcgcctgca aaagctaaaa ctatagaaaa atttttaggg aaaagtcatt acacagttaa
     1021 agcatcagtt ggtcatgtca gagatttacc taaaagtaaa ttaggtgtag atatagaaaa
     1081 taattttgaa cctcaatata taaatataag aggaaaaggc gatgtaataa aagaattaaa
     1141 aaaagaagct aaaaaatcta aacaagtata tctagcaact gaccctgata gagaaggtga
     1201 agctatatca tggcatttag ctcatatact aaatttagat gaatctgagg attgtagaat
     1261 agagtttaat gagataacta aggatgcaat taaaaaagca attaaacatc ctagaaatat
     1321 tgatataaat ttggttgatg cacaacaggc aagaagagtg ctagacagac ttttaggtta
     1381 tcaaataagt cctatattat ggcaaaaggt tagaaaaggt cttagtgcag gtagagtgca
     1441 atctgttaca acaaagttaa tttgtgacag agaaaaagaa ataaaggcat ttattccaaa
     1501 agaatactgg acaatagatg ttgaggctaa gacagtaaaa gacaaagata taactttgaa
     1561 attttatggt aaaaatgatg aaaagattga attagaaaat gaagaagctg taaatgaaat
     1621 actaaaagaa atggaaggta aagatttaaa ggttgagaag atagaatcta aatcaagacg
     1681 taaatcagct ccaaaaccat ttacaacaag tatgcttcaa caagaaggag caaataagtt
     1741 attttttact actaaaaaaa caatgatgat agcacaagaa ttatatgaag gtatcgacat
     1801 agaaggtgaa ggtactgttg gtcttatatc atatataaga actgactcta aaagaatatc
     1861 tgatgaagca aaagaaaaag ctaagggcta tatcgtaaac aatttaggag aaaactacta
     1921 taaaaacact ggtgataaaa aagaaaaaaa ggatactaaa aaggttcaag atgcccatga
     1981 ggcaataaga ccaacctcag ttgatagaac tcctgagagt ataaaagcct cattgagcaa
     2041 agaccagtac aagttataca atttaatttg gagaagattt gtagctagtc aaatggaaga
     2101 ttcagtgttt gatatactaa atgtagaatg taaaataggc gatattgtat ttaaggcaac
     2161 tggttcaaaa atgaaatttg atggttatac caaggtatat aatttttcag aaagagaaga
     2221 caaaatttta ccaagtatag aaaaaggaga tatattaaat atagaaaata tattgccaga
     2281 ccaacatttt acacaaccac cagcaagata tacagaagct agtttagtaa aaacactaga
     2341 agaactaggt ataggtagac caagtacgta tgctccaaca atagctacta tactgaatag
     2401 agagtatgtt gaaaagcaag gaacaagtct atgtccaaca gaacttggaa taatagttac
     2461 tgatattttg gaaaataatt tccataaatt tatagatgta gattttactg ctgatatgga
     2521 aagtaaactt gatgttatag aagaaggcaa tacagattgg aaaaagattg tatcagaatc
     2581 atatgcacca cttaaagagg ctattgaaga agctcttgaa aatatagaaa aagtaaatat
     2641 ggatgaagaa actgatgaga tttgcgagaa ctgtgggtct aatatggtta tcaaatatgg
     2701 tagatttgga aagtttatgg cttgtaaaaa ctatcccgat tgtaaaacaa ctagaccaat
     2761 tatgaataaa attggtgtta aatgtccaaa atgtgaagaa ggagatatta tacttagaaa
     2821 atctaaaaga ggtaaagctt tttatgggtg ttctaattat ccaaactgtg actttgtagc
     2881 ttggaataag ccaacaggag agttttgtga gaagtgtggt tcatatatgg ttgaaaaagt
     2941 gactaaatct gaaacaaaaa cagtgtgttc taataaagaa tgtaataaag agacaaaaga
     3001 gcataatgac ggaaaagagt gaaaattgaa aaaaatcaca taattctctt gaaaaaggtt
     3061 ccaatctatg ataatctttc aagtgtaaac aactaataga aaaataaatt ttcattaaat
     3121 gtgaaaataa tattagaaaa tataaaaggt tattaaaagg cctaaatttc atgaggagat
     3181 gattaaatgg caagtgaagt gttacaaaaa acaaggaaaa taaataaaac attacaaaca
     3241 agtggtggaa gcagtgtctc ttttgattta ctggccggag cattgggcga cgttttaagt
     3301 tctaatgttt atgtagtaag tgcaaaaggt aaagtactag gtcttcattt aaatgatgtt
     3361 caagacagtt cagttataga agatgagtat actaagcaaa agaaattttc agatgaatat
     3421 actcaaaatg tgttaaaaat tgatgaaaca ttagaaaatt taaatggtga gaagatatta
     3481 gaaatctttc ctgaagaaca tggaagatta caaaaatata ctacagtagt tccaatatta
     3541 ggaagcggtc aaagattagg aacattggta ctttcaagat attcaaattc attcaatgat
     3601 gatgatttag taatagctga atacagtgca actgttgttg gtcttgaaat attaagagca
     3661 ataggtgaag aattggaaga agaaatgaga aagaaagctg tagttcaaat ggcaataggc
     3721 actctgtcct actccgagct tgaagcagtt gaacatattt ttgctgaatt ggatggaaaa
     3781 gaaggtctac ttgtagcaag taagatagct gatagagttg gtataactag gtctgtaata
     3841 gtaaatgcac ttagaaaatt tgagagtgca ggtgtgatag aatcaagatc attaggtatg
     3901 aaaggtactc atataagaat acttaatgac aaacttacag atgaattaaa aaaattaaaa
     3961 aacaatcaat aatctatatt ttataggttt agattagaaa aggtatctat ttcaaataga
     4021 tacctttttt agacgaatta ttaaggtaat gataagtata ttaaaaagga gtgagaaaat
     4081 gcctttagca gataaaataa gaccaaaaac aatgaatgat gtaataggtc aaagtcatat
     4141 aataggtaat ggaaaaatat tgtctaagat attacaatct aattttttac caaatatgat
     4201 attttttggt cctcctggtg ttggtaaaac tacagtagcg gaaataatag cagaaagaag
     4261 caataaaagc ttctataaaa taaatgctac taattcatct ttagaagata taaaaaaggt
     4321 gatatcagag cttggaagta taaataatgt aaatggagtc ctattatata tagatgaaat
     4381 acaaagtttt aataaaaaac agcaacaatc aatattagaa tttatggaaa atgggagtat
     4441 aactttgatt gctagcacta cagaaaatcc atatcattat gtatataaag ctcttttaag
     4501 taggtctact gtatttgaat ttaaaccttt agaaaaactt gatgtagaaa aaggactgaa
     4561 aagagctgtt gaagtattaa atgaagatag ctatatggat attgagtgta ataataatgc
     4621 tattgaagat atagcaattc tttctgatgg agatatgaga agagccttaa atattttaga
     4681 agtggtagtc tattcaacta aaccaaataa ggataatata acttatatag attcagatgt
     4741 aataagggca tctacattta ataaaattat aaactatgac aaaaatggtg atagtcatta
     4801 tgatatttta agtgcatttc aaaaatctat aagaggaagt gacccacagg catctataca
     4861 ctatctagca agacttataa aaggtgggga tttgataagt atatgtagga gacttttagt
     4921 aatagctagt gaagatatag gtttagctta tccaaatgct attgtaattg taaaggcatg
     4981 tgtagactca gctatgcaac ttggatttcc agaagcgaaa ataccattag cagaagctac
     5041 tattcttttg gctacatctc caaagtctaa ttctgcatgt atagcaataa tgaaagctat
     5101 ggatgaactg gataaagagt ttgttaagga tataccaaat agtataaagg attctcacta
     5161 ttctggttct aaagaaatag gtaggggttg tggatacaaa tatcctcata attttaaaaa
     5221 tcattatgta aagcaacagt atttaccaga tagcataaaa gatagtattt attacatacc
     5281 acaagaaaat aaaactgaga aaaatataaa aaaatattta gaatatctaa atagtgattt
     5341 ttagactgaa ctttatgtat cgggggtaga tattatgatt ataaaactta actcaactta
     5401 tcacagtaga gttatgaaat acttaaaaaa agaacctgag tacaatttgt ttataatagg
     5461 ggatattgaa agatatggat atggaaataa ttttttaaat atatgggcag atgtgggtga
     5521 acatggtgaa ataaaggcaa tattacttaa atattttgaa tttatgatat tttattctga
     5581 tggagagtat gatgttgaag gtttttataa tctacttcgt aatactaatt acgaagaaat
     5641 atctgggaaa atatgtgctg tagatgcttt agcaaagaga ttaggactta ataatttaaa
     5701 ggtagttgat ttttgcaaac ttcaaactaa aaagtttttg atagataata attgtaatgc
     5761 aaaggttaaa agaattaggc ttggaaatct aaaaaagaca gtgaaacttt atgacttaat
     5821 agatgaattt cacagtacaa ctttagagaa tttaaaaaat ggactaagga caggtagagg
     5881 atattgtata gagattaaca aacaggtggt gtcaatggca aagagtactt cagaaaatag
     5941 gactcatgca atgataattg gagtaggaac acatcctaaa tatagagcta aaggacttgc
     6001 tacaaaatgc ctaattaaac tatgttcaga gttactgaga gaaaacaaaa ttccttgttt
     6061 gttttatgat aatgaagagg ctggaaagat atataaaaaa ctaggatttg aaaacattgg
     6121 aaaatgggga atttattcaa aataaaaaat taaaattaat cttgacaaaa ttagtagggt
     6181 ataataaaat tatcttgaaa gtaattccta ctaaaacagt atgaattaga tttctatata
     6241 gaggtgataa tatatgaagt tgtctactaa aggtagatat ggactaaaag caatgtttga
     6301 attagcatta aatcaagaca atggaccagt atcattaaaa tttattgcta agaagcaaaa
     6361 aatatcagat cagtacttag aacaaatatt ttcatcttta aaaaaatcgg gtctagtaaa
     6421 aagtgtgaga ggtgctcaag gaggttattt gctttcaaag aatgctgaag acataacagt
     6481 aggagatata cttgtagtat tagaaggacc agttgcatta tctgactgtg tactagatga
     6541 agatgtatgt gaaaactcaa acatgtgtgt aacaaaaata gtttgggaaa agatgaaaaa
     6601 gggtatagaa gatgttatag attcaattac tcttaaagat atgataaatg actataacaa
     6661 aaataaatta gaaaatgata taacaaatat taaaaaatag atttggagat gaatgtaatg
     6721 gaaaaaagaa gattgtatat ggattattct gcaacaacac ctattaaaaa agaagtattg
     6781 gatgcaatga tgccatacct aacagattat tttggtaatg catctagttt tcacacattc
     6841 ggaagagaag caaaagatgc tttggataaa gcaagagaac aagtagcagc ccttataaat
     6901 gctgaaccta gtgaaatata ttttacagca ggtggttcag aaagtgataa ctggacacta
     6961 gagggtgtag cttatgctaa taaaaacaaa ggtaatcata taataacttc aaaaatagag
     7021 caccatgcaa tacttcatac atgtgaatac ttagcaaaac atcatggatt tgaaataact
     7081 tatttagatg ttgatagcga aggaaaagta gacttaaagc aactagaaga ttcaataaaa
     7141 gatactacta tacttataag cataatgttt gcaaataatg aaataggaac tatacagcct
     7201 attaaagaaa tatccgaaat agctaaaaaa cataagatat tattccacac tgatgcagta
     7261 caagctacag gtaatattcc agtagatgtt aaagaattag gaatagattt gatgagtatg
     7321 tcttcacata aaatatatgg accaaaaggt gtaggagctt tatacataag aaaaggtgta
     7381 agacttcata actttgtcca tggtggagca caagaaaaaa gcaaaagagc tggtacagaa
     7441 aatatacctg caatagttgg atatggaaaa gcagcagaat tagctaagga aaatatgcaa
     7501 aatcatgtag aaactttaac tcgtcttaga aataagttaa tagatggtgt gttagaaaga
     7561 ataccttaca caagagtaaa tggtagctta gataatagat tgccaggaaa tgcaaacttt
     7621 gcattccaat ttatagaagg tgaaggaata cttttattat tagatatgtt aggaattgct
     7681 ggttcaagtg gttctgcatg tacttctggt tcattagacc cttcacatgt acttcttgca
     7741 ataggtttac cacatgaaat agcacatggt tcattaagac ttacagttgg agattttaca
     7801 acagatgatg atatagatta tatactagag aacttaccaa aagtaataga aagacttaga
     7861 agcatgtcac cactttatga tgatgcgaag aaacaaggtt tagtaaaata gacataaaga
     7921 taaagcataa aaaatagaca taaattgagt aggaggaaaa gataatgcaa tatagtgata
     7981 aagttatgga acattttatg aatccaagaa atatgggaga aattgataat gcaagtggtg
     8041 taggtgaagt tggaaaccct acatgtggag atataatgaa gatattttta gacatagatg
     8101 gagatgtaat aaaggatgtt aagtttaaaa catttggctg tggttcagct atagcaagtt
     8161 cttctatggc aactgaaatg ataaaaggaa aaactataaa agatgcatta gaacttacaa
     8221 ataaagctgt tgcagaagct ttggatggtt taccaccagt aaagatgcat tgttcagttt
     8281 tagctgaaca agctgtaaaa gcagccctta tagattatgc tcaaaagaac aatatccata
     8341 taccagaatt ggatggatat gttatagatg atgctcatga ccatgatgtt gaagaagaag
     8401 aataaaaata agtgattgtg ttatgagaag tttacaatga ttaaaaagaa gtactaatga
     8461 tataattagg acataaaaaa tagctgtttt taaaatggct attttttatt gcctaaatta
     8521 agtattttaa cttgatttag aggttaatta gttaacaaaa taatccataa gattcaaata
     8581 ataaagatga ttattttttt agaaaataga acaagctata ttttaaataa gtacaatact
     8641 tgctctatat ggtataataa taagtaatgc aaaagatgag gtgaaatttg tttatgaata
     8701 aaaaagtaat gataggtatg agtggaggag ttgatagctc tgtagctgca tatcttttaa
     8761 aacaacaagg atatgatgtc attggtgtta ctatgaagtt gtggcaagat gatgatgttg
     8821 ttgaaattga aggtggttgt tgctctttat ctgctgttga agatgctaga agggtagcaa
     8881 ataaaatagg tataccattt tatgttttaa attttagaga agtgtttaaa gaaaaagtaa
     8941 tagattattt tatagatgaa tatttggaag gaaaaactcc taatccctgt atagcttgta
     9001 ataaacatat aaaatttgat gatttttata agaaggctag acaaataggt tgtgattatg
     9061 tagctacagg tcactatgca aagatagaaa aggatgaaag tacaggaaga tatttgctaa
     9121 aaaaatctgt tacagataaa aaagaccaaa cttatgcact ttataatctt acacaagagc
     9181 aactagagca tactttattg ccaataggtg actatgaaaa agatagagta agagaaattg
     9241 ctaaggaaat gggtatggca gtacacaata agcctgatag tcaagaaatt tgttttgtta
     9301 aagataatga ctatgcaaat tatgtaaaaa aacattctaa aaagcgtatt gaagaaggtt
     9361 tttttgtaga tactaaaggc aatatacttg gaaaacataa gggtatatta tattatacta
     9421 taggccaaag aaaagggctt ggaattactt ttggaaaacc aatgtttgta atagatataa
     9481 atccaataaa taataccata gttcttggag acaatgaaga tttatttaaa aaggaactta
     9541 ttgctaaaga tgttaacttt atatcaatag atacgttaga agaaccctta agagttcaag
     9601 ccaaaataag atattcagca aaaccatcac cagctactat tcatagagta ggagaagata
     9661 ctattaaaat agtttttgat gaagctcaaa gagctattac taaaggtcaa tctgtagtaa
     9721 tgtatgatgg agatattgta gtaggtggag gtataatcga gaaaagttta taaaaacatc
     9781 taaaaaatta aaaaaataag caataaagta tattaagagt aaataaagtt tataaaataa
     9841 atttattgta tatgaaaata tagtattgat taattttgta aaatttagta atataattag
     9901 tatattaatt aaaaaacaaa tacagtgata aggaatagta aaatatttga tgtcttacag
     9961 agagagggaa atgctggaag cccttagaat atgatatttg aagcagcctt ttagtagcaa
    10021 tcttgaacaa tttatctttt ataaagaaga tttaagtaga gattgacggt agttacgtta
    10081 tagctaaggt tttccgaaga tatcatactt tgtatgataa ttagggtggt accgcgattg
    10141 tcctcgcccc tataaattta tttataggga cgaggttttt tatttaaacg aaaaaataaa
    10201 atttattagg aggaacatga tatggaaaag atgggattaa atgaaataag aagtaagttt
    10261 ttagaatttt ttgagtcaaa aggtcattat gtagcaaata gttattcttt agtaccaaac
    10321 aatgataaga gtttgttact tataaactca ggtatggcac ctttgaaaaa ctacttttca
    10381 ggtgtagaag tacctccaag tgtgagaatg tgtacatctc aaaaatgtat aagaacagga
    10441 gatatagaaa atgtaggtat aactgcaaga catgctacat tctttgaaat gatgggtaat
    10501 ttctcatttg gagattactt taaaagagag tcaatcaaat ggggatggga atttgttaca
    10561 gaatggctaa atataccaga agataagata tgggtgactg tatatgaaga agatgatgat
    10621 tcttatgata tatgggcaaa agaaatgaat ttcccagaag aaagaatggt tagacttgga
    10681 aaagatgata atttctggga aataggtaca ggtccttgtg gtccttgttc agaaatatat
    10741 tttgatagag gagaagaata tggatgtgac aatccagact gtaaaccagg ctgtgattgt
    10801 gataggtatt tagaattttg gaaccatgta tttactcaat ttgatagaga tgaagaagga
    10861 aattatagtc tattagaaaa caagaatata gatacaggta tgggtcttga aagaatggga
    10921 tgtattatgc aaggcgtaga cactatcttt gaagttgata caattaaatc tatactagaa
    10981 gcagttgaga agttaacagg agtaaaatat ggagagaatc caaaaaatga tatatcaata
    11041 agaataataa ctgaccacat aagagctgta acattcttag ttagtgatgg agtgcttcca
    11101 tcaaatgaag gaagaggata tgttcttaga agactcctta gacgtgctgc tcgtcatggt
    11161 aagttattag gagtaaaaga attattttta cagaaactaa tagatgaggt tataaaagta
    11221 aatgataaag catatccagt tttagttgaa aaagaaagtt atattaaaaa agtagttgga
    11281 atagaagaag aaaaattcaa tgaaactata gaccaaggaa cagaaatatt gaactcttat
    11341 atagaagtat taaaaaatga aggaaagact gtcctaagtg gtcaagaagc ttttaaacta
    11401 tatgatactt atggcttccc tatagactta actaaagaaa tattagaaga agaacatcta
    11461 tctgtagatg aagaagcttt taatgaagaa atggaaaagc aaaaagaaag agctagaaat
    11521 gctagaggaa atatggatgg agaaagttgg aaggaagacc ctctatcaaa attagaatct
    11581 acagttgaca gtacatttaa tgggtatagt gaaatttatg gtgaaggaac tattgaagct
    11641 atagttaaag atgatgagct agttcaaagt gcagaagaag gcgataaggt atctatagta
    11701 ctagataaca ctactttcta tcctgaaggt ggaggacaag ttggagattg tggattgata
    11761 actaatgaaa acttagtttt agaagtatta aatactaaaa aaggtgctaa taatagtata
    11821 aaacatatag gtataataaa atcaggaaga ataagtaatg gggacaaagt aaagacactc
    11881 gtagatagag aaactagaat gtcagctgca agaaatcaca gtgctacaca tttacttcat
    11941 aaggcattaa gagaagtatt aggagaacat gttaatcaag ctggttcttt agtaactcca
    12001 gaaagactta gatttgatat tactcacttt gaagcaatat caaatgaaga attaaaagta
    12061 atagaagaaa aagtaaataa tgttatttta tcatctttag acataaaatg tgatattatg
    12121 aatataaagg aagctaaaga aaaaggagct acagcactat ttggtgaaaa atatggtgat
    12181 gaggtaagag tagtttctat gggagattat tctacagaac tttgtggagg aactcacttg
    12241 acaaatactt ctcaagtagg aatgttcaaa atattatctg aaggtggagt agcagcaggt
    12301 gtaagaagaa tagaagcaat aacaggaaaa gctgtttatg aatacctaaa agaaagagat
    12361 ggaattatct ctgaagtttg tgtaaactta aaatctaaag aagataattt aattcaaaga
    12421 ataagttctt tattagaaga aaataaaaac ttatctaaag aattgcatga tatgaaagct
    12481 aagatgagtt tacaatcagt tgattctatt tttgattcta aagttgaagt aaatggagtt
    12541 aatttgataa ctaataaatt tgaaggtatg gatatggata cactaagaga aacagcagat
    12601 aatctaagag ataagcttgg ttctggtgta gtagtacttg caaatgtagt agatgataaa
    12661 gttaactttg tagttactgc cactaaagat gtattagata agggaataca ttcaggtaat
    12721 attgttagag aagttgcaaa aatagctgga ggaaaaggtg gaggaagacc taatatggca
    12781 caagcaggag ctagtgatgt atctaaggtt gaccaagctt taagctatgc aagtgaggtt
    12841 attaagacac aagttaaata acattcactc aaggaggttt ttagtttgga aaaagattta
    12901 gactacacta tgaaatttga aggaatacca gaagatagga tgagtgtagg agatactatt
    12961 gattttgtat ataaagcatt agttgaaaaa ggatataacc ctataaatca aattataggg
    13021 tatctccttt ctggtgattc tagctatata actagtcata aaaatgcaag agctataatt
    13081 aaaaaatttg aaagagatga gatacttgaa gaagtaatca cgcactatct gaatagaaag
    13141 taggtcaaat aaatgagaaa aaagattatg tcgttgtttg caatagtcat tatgattttt
    13201 gtatcaactg ctagctatgg ttttgctgaa gaaaaaggca aggtcatatt tattgatatg
    13261 aataggacca atcttggaag tatgatgaat attccaatct tgaaaaatga gatagaaaag
    13321 agaggctatg tagctctcat gaatataaga ggggatcaag gtactgatga tagaaggtct
    13381 tgtgcaagta tgggtgcagg aacaagagct agtgtaacta ctgaagacta tatcaatttt
    13441 caaaaagtag accaagatac tgctaaagct tttgaagtcg caacaggtca aaaagctaaa
    13501 gaaataaatg atttaggcat taatagatct ataaatgaaa atcttgagta tggagaatat
    13561 ggagcaaatt tagggtcatt aggaaaaact ttatcagaaa atggtttaaa gacatctgta
    13621 attggaaatg ctgatataat tgaaaataat caattagtta aaaacagaaa cttatgttta
    13681 gtagctatgg accaatatgg aaggattgat tcaggtaata ttgataatat taataagaaa
    13741 gattctaaaa tgccatttgg aatatctaca gattatgata aattactaga tgaaactaaa
    13801 aaatcctata aaaatagtga tgctatattt gtagaacttg gagatacata tagattagat
    13861 atgtatagat taaatctaaa tgataatact tatgatacaa tgaagaaaaa tattgtaaaa
    13921 aatataaata aatatcttga acaagtattt tctatggttg gagaaaatga cactgtttat
    13981 atagcaagtg catttccaag tgacctagat tataagaata agagaagact ttctccaata
    14041 ataaaattta gtggagaagg gaaaggtcta ctggaatctg ccacaacaag aaaggctgga
    14101 attgttgcca atttagatgt tggagtagat atactaggta catttggtct tgaaaataaa
    14161 gagatggtag gtagaagtta tactttagta aataaagagg acaatattag ctatctaaaa
    14221 tcactttatg aaaggatagt gtctatatct tcaataaggt caactattat aaatggcttt
    14281 gtaggcgtaa tatctgcttc ttgggtaatt gccatgtttg cgattttctt tagaaattat
    14341 atgccacata aagagcgtgt attcaaagta cttaaggagt ttataaaatt aggcataata
    14401 atgccactat catttatgtt agctcctttg atgaatttta aaactccaat ttcaatatct
    14461 ttgggaatat ttgcaactac gctaatatta tacttgttag gtagaaaatt atttaaggac
    14521 aatgatataa agcacatggg attttttgca acacttacta ttttagtcat agttatagat
    14581 tctgtatttg gaacatactt aatgcaaaat aacataatga gttatgatgc tgtcatagga
    14641 gctaggtact atggtgtagg aaatgaatat gaaggtgtaa ctatagcaag tgcagtattt
    14701 ggcttagcag tattattaca ttataagaag gtatctaaat tacttgttgt aattttttca
    14761 ttaataatac ttataactag tgcatatcct tcaatgggtg caaatgttgg tggagctata
    14821 tcagaatgtg tagcatattt gttgtttata atgcttatat atgacattaa aatagatttt
    14881 aagaaagctg tattattagc tgtttcagct gtagttgtcg tttttgcatt tgcagcttta
    14941 gatatacttt caggtagtga atctcattta ggtatgtttg tacagagaat tttcctaaat
    15001 ggaccaggag agatattaca aacatttgga agaaaaattc aaatgaatat gcaactagca
    15061 cagacaagtg tatgggtaaa tattttactt gtaggaattg gagtaattgc agtgcttata
    15121 tttaaaccat ctagacactt tagaaagata atgaataatt ttccaatctt gtttaaaggg
    15181 tttattgcat ctatggttgg ttgtataatt actcttttag ttaatgattc tggaatagtt
    15241 gctgcatcta cagcatctat atatatactt ataccactaa taataataag tataaatatg
    15301 ataatatttg aaaaaaataa ctaaaaaaat ttttactgtt catataggat ttaaataaaa
    15361 aatgcaatat gattattaat gtaaactgga tgcttacata aaaaatcata ttgcaatttt
    15421 tatgattgat aatgtaaaat tctatgaagc agtaagcctg tgaccttagt tatgagaggt
    15481 tcagaatatg tatttaatct atagaatata aatagataaa ggaggaacta tgttagatgg
    15541 aagaataatg ggtcttgatg ttggtgataa aactattgga gttgctgtga gtgatttgat
    15601 gggattaact gcacaaggtg taaaaacaat aaaaagagtt ggcaagaaaa aagatattga
    15661 agagctaaaa gcaataataa aagaaaaaca agtaaataaa attgtatcag gattaccaaa
    15721 aaatatgaat ggaactttag gtccacaagg agaaaaagta atcaagtttt gtgaattggt
    15781 aaaagcagaa acaggtattg atgtagagtt ttgggatgag aggctttcta cagtagcggc
    15841 agagaggtct ttattagaag cagatgttag tagacaaaaa agaaaaaagg ttatagatat
    15901 gttggcagca gtaataattt tgcaaggata tttagatttt aaaataaatt cataaaagaa
    15961 ctataaaatt gaatacttat gatatataat ataaataata aaaataaaaa taaatttgag
    16021 gtgaatgaca ttatggaaga aaatataata aatttaatag acgaaaatgg cgttgaaagc
    16081 caatttgaaa taatattaac attagaagca gaaggaaaag agtatgcaat attaatgcct
    16141 ttagatgatg aagaagctga agaagcttta atctttagaa ttgatgagga tgaagaaggt
    16201 gaaatcttaa tacctttgga aagtgatgaa gagtatgaaa ctgttgtagc tgtgtatact
    16261 gctataatgg aagaagaagg tttaaattac gacgaagatg aatcaaatgg acttaactag
    16321 ataagtaatt aagtttgtat ataaaaatca gatatttatt atatttgctt taattaaacc
    16381 ttattaattg taaaagcatt tatagtatat atctgttttt ttgtttttta ttaaaaatac
    16441 caaaaaattt atgaattttg gtgaataata tatcttttat gggtatatat ttatattatg
    16501 agaataaatt aaatttattt aaatgtcttg acaaaaaatg aattatcaat tacaatatta
    16561 tttaataaag attatcaatt gaaaatagta agggtgatgt tatggcaaat acaatggatt
    16621 tattaaaaga caagttaaaa gaaactggtt ttaagattac gccacaaaga agagcaatag
    16681 ttgaaatatt gcttaaacat gaccattcac atctaagtag tgaggaaatc tatgatttag
    16741 ttagggttga ctgtcctgaa ataggcttag ctactgtata tagaacaatg cagctattgg
    16801 atgaaatagg attaatatca aaactaaact tagacgatgg atgtataaga tatgagatta
    16861 gtctacataa agaagactgt cataatcatc atcatcttat ttgtaaaaat tgtggaaaga
    16921 taatggaagc taaagaagac cttttagaca atatagaaaa agagatacaa tcattgtaca
    16981 aatttaaaat acttgatcat gatgttaaat tttatggact atgtgacgag tgtaatggag
    17041 taagcgattc agaagaataa acttctattg cttaatgtcg atggaagaat agtatagaac
    17101 ttttatattc tatgctattc ttccattttt tattataaac catctataaa atgcaaatat
    17161 acagttttca aagtgattat agttttaata ttgaaaatat aattaatcct ataagtacaa
    17221 aaatgaatga tttttctatg aaattactaa atggtcctcc aacagttaaa aaccctaaag
    17281 aaattttctt ttcgcttagg ggataaaaaa ttggtattcc ttgctttgta aataaatctc
    17341 ctaaaaacat atggcttgca taaccaatcg taccataaaa agataaatta ttaatattag
    17401 tgattaattc tatctgtttt agtaaaaaat atactattat agttgcaaat atgctatgag
    17461 aaaaacttct gtgctttaac caggggaaaa ttgacaacat tagacaaaaa attgtaaaat
    17521 atatttcaac ttctatgatg tataagcata tagctaatag tataaatatt aatgatagaa
    17581 aaacttttct tagaaatgta tgggttagtt ttgcttcaat aattataatt gcaacaaatg
    17641 taattattgc gcttaaataa aaattatcat ttatatttat tgaaataaag aaaataataa
    17701 tattaacagc atatataaaa attctatata tgtatttgga tacattgttt tttagaatga
    17761 aatttgagac catagaatta gatttatcca aatcgggtag tacagcaaaa gtagctgaaa
    17821 ctaaaatatc aactaaagat ataggaattt taaatattaa agatgtctgg atagctgtta
    17881 agattcctat tgcacaatga gttttaccac gcataaatcc tcctaaatta tccgatatta
    17941 aatcatataa tatttagagg atatcaaaat aaaataggtt tgtcaaaata atacgttaaa
    18001 tgtattttaa atatagctta aaatatgata cgaattatgg tacaataaga ataactggta
    18061 gcgagtgcgt gatttgatat tatttatgat tgtagaacgt gttaaaaaat taaatagaca
    18121 gaaggagatg atgcaattgt tcaaaaagaa caccaataag ataaaggtaa tggccttggg
    18181 tggacttaac gaagttggaa aaaatatgac tgttgttgag tataaagatg aaataattgt
    18241 tatagatgct ggacttagtt ttccagagga tgaaatgtta ggagtagata tagttatacc
    18301 agatataact tatttagtaa aaaataggga taaaataaag ggtatattta ttacacatgg
    18361 acatgaagac catataggag ctcttccata tatattaaag aaaataaatg tacctgtata
    18421 tggggcaaga cttagtatag gtcttataca agtaaaactt aaagaacata agatgaataa
    18481 tgttaagtta aatgttatag gtcctagaca agttataaag cttgataata tggaagtaga
    18541 gtttttgaaa aataaccata gtataccaga tgcttactct attgcaatac acacagacca
    18601 aggtataata taccacacag gagattttaa gattgacttg actcctattg atggagatgt
    18661 tatggatatg catagaatct gtgagttgag caaaaaagga gttcttttaa tgctggctga
    18721 tagtactaat gcagaaaaac caggatttac tatgtctgaa aagactgttg gggtaggtct
    18781 tgacgaatta tttgcaaaag gaaatggcag aagaattata gttgctacct ttgcatccaa
    18841 catacacaga ttacaacaaa taataaatac tgctgagaag tttaatagaa aagttgctat
    18901 atcaggtcgc tcaatggtca atgtagttgg agttgcaaaa gaattaggat atctagatat
    18961 ttctgatgac atgcttatag atttaaatga tatatgtaag tatgaagata gtgaattagt
    19021 gataataaca actggttcac aaggtgaacc tatgtcagct cttgctagaa tggctttttc
    19081 agagcacaaa aaagtagaaa taaagagtgg cgatttagtt ataatatctg ctcatccaat
    19141 accaggaaat gaaaaattaa tatcaagagt aatcaatttc ttatttgaaa aaggagcaga
    19201 agtagtatac agtgatatag ctgatataca cgtttctgga catgcctgtc aagaagaatt
    19261 aaaacttata catgcattag ttagaccaaa gttttttatg ccagctcatg gtgaatatag
    19321 aatgttaaaa agacatgctg aaatagctga acagcttggt atggataaag aaaatatttt
    19381 tgtcatgcaa acaggagatg ttttagaatt agataaaaac tctgctaagg tagcaaatcg
    19441 tatacaaaca ggaaacatat tagttgatgg tctaggagta ggcgatgtag gtaatatagt
    19501 tttaagagat agaaagcatt tatcagaaga tggtcttatg atagttgtag ttactatttc
    19561 taaggatgag ggtaaagttt tagcaggtcc agatataatt tccagaggat tcgtatatgt
    19621 aagagaatca gaagatttaa tggatggagc aaaggatata attaagaatg ttctaaatga
    19681 atgtgaagag aaaaatatta aagaatgggc ttatttaaag aataatatta aagaaaacct
    19741 aaaagaatat ttatatcaaa aaactaaacg aaatccaatg atacttccta tcataatgga
    19801 agtataatat aaaaaaacta taaatttgaa aattagacct cttgtaattt ttaaagtaat
    19861 atcaaaaatc caattgatat tatcttaaga attacaagag gtctttttaa tacttgattt
    19921 aaaaaaatat atatattaca taaaatacca attataacaa tcttttatta gtaaaaacta
    19981 cttatatata aattttactg ataggaggat tcttatggat gatgtttcaa gacaaaatgc
    20041 tataaaagct ttaaaacaaa caaaaatgga aatagcagga gagtatggaa tgaattatga
    20101 agatgctttt gaaataatag aaaatgcgtc taataaaggt gttttagaag gttactttaa
    20161 aaagcttgaa aagaaaaaaa atttaggtca aggtatatct aggcatttag aataaaaaaa
    20221 gttaaatgtg agaaaataag tttaaataaa ctttaagagg tgacatcgat gaaaaaatta
    20281 gctagtactg ctttggctat attaattgcg cttactccct taagcttttc ttttgcaaac
    20341 aataaggaaa atgctgatgc caaccaacta aatatatctt caaaatcagc aattttgatg
    20401 gatgttggta gtggacaaat tttatatgaa aaggatgctc ataaaaagct tcctcctgct
    20461 agtgtaacta aggtaatgac tatgctactt atagtagaag ctttagattc aggcaagatt
    20521 aaacttgatg atgaagttca agtaagtgaa actgcatcta gtatgggtgg tagtcaaata
    20581 ttcctagagc caggagaaac tcaaaaggtt gatacattgt taaaaggtat agctgttgct
    20641 tctgcaaatg atgcttgtgt tgctatggct gaacacttag ctggaagtgt tgaagggttt
    20701 gttgatagaa tgaatgctaa agcaaaagag ttaaatatga atgatactca ttttgcaaat
    20761 acaaatggac ttccagttgc aaaccactat acatctgctc atgatatagc tttaatgtct
    20821 agagaactat taaaacacga aatgataagt aaatacttaa ctacatggat ggataaggtt
    20881 gtagtaggga agaaacaagt tacagtaggt cttgcaaata ctaacaaact tataaagcat
    20941 tatcaaggag caactggtgt taaaactggc tttactcaag aagcgaaata ctgtttatca
    21001 gcatctgcaa agagaggtaa tactcatcta gttgctgcaa ctttaggagc agagacttct
    21061 ccagaaagat ttaatgatgc ctctagcctt ttaacctatg gatttgctaa ttatgaaagt
    21121 gttaagttat gtagtaaagg tgataatata gcaacactta cattggataa ggctgatgaa
    21181 aacaaagtta aattggttgc taaagaagat ttaaatgctc ttattaaaaa aggaagttct
    21241 aaagaatttg agaagaaaat tgaaatagta aaaaatccta agatgccaat taaaaaagga
    21301 actgttttag gaaagataaa gatatgtaaa gataaaaaag taataggcga agtggaatta
    21361 ataaatacta aagatataaa taaggcaagc tatctacaaa tgcttcaaag aataattgat
    21421 aatatgatat aaataattat cagatgaaat ataattataa tacacaaaga tatgattata
    21481 atacacataa aaaataccct tatgtaatta tttgccatag gggtattttt atttactata
    21541 tagaagtatt ttataatctt atgattttta tagaaatata gaaaagttta tgtatcttaa
    21601 aatatcattt gatttaatat catatatagg gttgtttaac aattatctaa gtaaaggcat
    21661 actaccagta agtttattca ctttcttttt attcccataa attgctatcc caaaatactg
    21721 taactcatct tctggaacac tagcaatctt ttgtgtaaat tcttcataaa gattgcaact
    21781 ttgtgctaca tctgaaaaat ctacaacagt taaatcgtta aattccaaag tatataattt
    21841 tttacgtaaa tcttttatta tttctttgtt tccttttaaa ataggtacag gaatgtcaat
    21901 aattcctaag tggctattac ctgttttgtc aattacatct ggcccaacag tttctggggc
    21961 atgttttcct agtgtaattc ccattatagc tgctgtattg gatataattc ccattggtaa
    22021 attttcgtct attaccatga cacattttga atttttattt tccattttta tttctccttt
    22081 ttaccttatt taaagttgtt tgaatcatca tttataaata tattttgata ctgctttggt
    22141 gtaagaccaa taaaattctt aaaaaagttt gtaaagtgac tttggtctac aaatcctgtt
    22201 tgtatcgcag catctatagg ttctacgcct ttttctaaaa gctttttagc tttgtttacc
    22261 cgtatatttt ctaggtatct atatggtgta atacctttta atttcgtaaa attacgaagt
    22321 agagagtact tattcatacc tgctatagtg cttaattcgt caagtattat atgttcagca
    22381 taattatttt ctagataatc acatactgct tgaatttcta tatttgtatt ttccaagtta
    22441 gattgcaaat ttggctctgt atgttcttca attaattgtt caattaggaa gaaaaaaagc
    22501 tcttcctttt taaaatccaa ttcttcttcc atgattatat agtgtaattc ttgtagtaga
    22561 ggtactaact cactgcgaaa tataacaggt tgattaaatt ttggtaaata atttttacct
    22621 gtaatttcaa agacggtttt tttcattatt tctggcttaa tattaataca tctgtaatca
    22681 agtactttat catctatttg ttcacaagta tgactatcaa aagggttaaa aagtagcaaa
    22741 tctccagtac ttgtggtata ctctttattt ttacaagata aatatctttg acctttttct
    22801 ataaaaccaa ttacataatg ttcatgaaaa tggtttggga atttttgcat tatgccttta
    22861 aatctatatg cttcaatttt taattcccta tcaaagcata ctgtgcgttc ttcttgtctc
    22921 acttaggtaa cctcctttca tgttatacaa agtgtaacac atattttttt atacttcttg
    22981 tataatattg actaagagac aataatgtca attcatgtca atataatatt acacttacag
    23041 taggtgtaat tattatattt tcacaatgaa tatgatataa taataaaagt tataaaataa
    23101 aaagcaaatt ttaattttat gcctagaagt taaaattatt ataatgtaaa ataattttga
    23161 ttataagcta tttaattgtt ataataatat taaaaattta atttaggaga tgtacattaa
    23221 tgttaagtat gaatatagga gaaatagtag caagtttagt tggtatagct atggcaatat
    23281 caatacatga gtttggccat gcttattcag ctcacttatt gggagatgat acagctaaag
    23341 cttatggtag aatgacatta aatcctgtaa gacatgttga cccaataggt cttttggcta
    23401 tgtttatcct taagataggg tgggcaaagc cagtaccagt aaatccaaat aactttaaaa
    23461 actataagat aggaaacctg atagtttcac ttgcaggcgt tttttgtaat gttttgactg
    23521 ctatactgtg tgtaattatc aataagtatg tgcatatgta tgctataaat acaatagctg
    23581 aatatgtgtt tttatatagt attggatttg cagcttttaa cttacttccg atacctccac
    23641 ttgatggatg gggagttata tctacatttg taccatataa atggaatgaa tatttgtata
    23701 aatttgaaag tatgagttat ataattttat taattgcttt atttactggt atgtatagaa
    23761 taatattaac accaatatat gcagtgttgc taaaaatagt atatttattt gtttaatata
    23821 ataaacagat taaagaggga ttgatatgaa gtataatata caattacaag tctatgaggg
    23881 accattagat ttactttatg atttaataac aaagcataaa atagatataa aggatatatc
    23941 cataatagat ataactaagc agtatctaaa ttatttgaaa atgctagata aaatggattt
    24001 a
//
LOCUS       DACULQ010000046        23480 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.046, whole genome shotgun sequence.
ACCESSION   DACULQ010000046 DACULQ010000000
VERSION     DACULQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.046"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            194..376
                     /locus_tag="JA154_07320"
     CDS             194..376
                     /locus_tag="JA154_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995675.1"
                     /translation="MHMEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNE
                     AFSTLEKELNISKANT"
     gene            550..1680
                     /locus_tag="JA154_07325"
     CDS             550..1680
                     /locus_tag="JA154_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAT7995676.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            1921..2271
                     /locus_tag="JA154_07330"
     CDS             1921..2271
                     /locus_tag="JA154_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization protein"
                     /protein_id="HAT7995677.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            2279..2728
                     /locus_tag="JA154_07335"
     CDS             2279..2728
                     /locus_tag="JA154_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutP"
                     /protein_id="HAT7995678.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            2830..3405
                     /locus_tag="JA154_07340"
     CDS             2830..3405
                     /locus_tag="JA154_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT7995679.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3398..4807
                     /locus_tag="JA154_07345"
     CDS             3398..4807
                     /locus_tag="JA154_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT7995680.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            4991..6424
                     /locus_tag="JA154_07350"
     CDS             4991..6424
                     /locus_tag="JA154_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase"
                     /protein_id="HAT7995681.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6442..7806
                     /locus_tag="JA154_07355"
     CDS             6442..7806
                     /locus_tag="JA154_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088420.1"
                     /note="with EutC catalyzes the formation of acetaldehyde
                     and ammonia from ethanolamine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia lyase large subunit"
                     /protein_id="HAT7995682.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            7819..8700
                     /locus_tag="JA154_07360"
     CDS             7819..8700
                     /locus_tag="JA154_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase"
                     /protein_id="HAT7995683.1"
                     /translation="MNEKDLKALVEQLVGQMLGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8720..9373
                     /locus_tag="JA154_07365"
     CDS             8720..9373
                     /locus_tag="JA154_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088422.1"
                     /note="carboxysome structural protein involved in
                     ethanolamine utilization; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein EutL"
                     /protein_id="HAT7995684.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9384..10085
                     /locus_tag="JA154_07370"
     CDS             9384..10085
                     /locus_tag="JA154_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine carboxysome strutural protein"
                     /protein_id="HAT7995685.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            10088..11557
                     /locus_tag="JA154_07375"
     CDS             10088..11557
                     /locus_tag="JA154_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HAT7995686.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11653..11940
                     /locus_tag="JA154_07380"
     CDS             11653..11940
                     /locus_tag="JA154_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088425.1"
                     /note="ethanolamine utilization protein; involved in the
                     degredation of ethanolamine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutM"
                     /protein_id="HAT7995687.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGATAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            12067..12828
                     /locus_tag="JA154_07385"
     CDS             12067..12828
                     /locus_tag="JA154_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HAT7995688.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12841..13470
                     /locus_tag="JA154_07390"
     CDS             12841..13470
                     /locus_tag="JA154_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088427.1"
                     /note="catalyzes the formation of propionyl phosphate from
                     propionyl-CoA in propanediol degradation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization protein"
                     /protein_id="HAT7995689.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13512..14237
                     /locus_tag="JA154_07395"
     CDS             13512..14237
                     /locus_tag="JA154_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAT7995690.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDGFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMNGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDILNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            14250..14522
                     /locus_tag="JA154_07400"
     CDS             14250..14522
                     /locus_tag="JA154_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutN"
                     /protein_id="HAT7995691.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14515..15066
                     /locus_tag="JA154_07405"
     CDS             14515..15066
                     /locus_tag="JA154_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAT7995692.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKICAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            15078..16169
                     /locus_tag="JA154_07410"
     CDS             15078..16169
                     /locus_tag="JA154_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088431.1"
                     /note="may be involved in the transport of ethanolamine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAT7995693.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            16171..16644
                     /locus_tag="JA154_07415"
     CDS             16171..16644
                     /locus_tag="JA154_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutQ"
                     /protein_id="HAT7995694.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            17157..17678
                     /locus_tag="JA154_07420"
     CDS             17157..17678
                     /locus_tag="JA154_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7995695.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            18057..19220
                     /locus_tag="JA154_07425"
     CDS             18057..19220
                     /locus_tag="JA154_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7995696.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGPDQSIDPIKPACDIVLKVSSIPVN
                     HISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMSNGVG
                     QVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGEILKE
                     YSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDILKLGCEFMAKYAVEFLNKNNN"
     gene            19254..20792
                     /locus_tag="JA154_07430"
     CDS             19254..20792
                     /locus_tag="JA154_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HAT7995697.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            20824..22026
                     /locus_tag="JA154_07435"
     CDS             20824..22026
                     /locus_tag="JA154_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAT7995698.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(22193..22792)
                     /locus_tag="JA154_07440"
     CDS             complement(22193..22792)
                     /locus_tag="JA154_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7995699.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            22962..>23480
                     /locus_tag="JA154_07445"
     CDS             22962..>23480
                     /locus_tag="JA154_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995700.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKD"
BASE COUNT         9453 a         2688 c         4277 g         7062 t
ORIGIN      
        1 tatttatttt atttctttac tattcaagtt ctaaatcaat aataaatttt gatatttaat
       61 tgtatcagga caaatctcta atttcataga ttattaatat aaaatcatag gttagtgtga
      121 aaaagttgaa atttaaatat tttaaaagat aatttttttg aactaatagg tataacaaaa
      181 atttataggg ggattgcata tggaaatcaa tgttatagaa attttcccta aagataaagc
      241 taaacttaat aaaatagaaa tggataaagc tagttggttt gtaaatataa taagtaaaaa
      301 atatcctaaa gaagctttaa atgaagcatt tagtacttta gaaaaagaat taaatataag
      361 taaagctaat acataagctt tgattattga ttttatatac tattggtttt atacgctatg
      421 acttgacatt atattaatca aatagtatat ttttaaatat agcacaatga agtgttaaaa
      481 gagcaatgga gctccataga taaagtctat ggagcttttt tgcgtattta acttaaggag
      541 gataggactt tgaataactt tgagattaaa actagaataa aatttggaga aggctcacta
      601 aaagctttgc aagaattaaa aaacaaaaat gtactaataa taacagaccc ttttatggtg
      661 aaatcaaaaa caatagataa aataacatct aatttaataa actcaacata tcaagttttt
      721 gaagaaatag taccagaccc accaatagaa gtagtagtag caggaataga agcatttaaa
      781 tctataaatc cagacgtaat aatagcatta ggtggtggtt ctgcaataga tgcagcaaaa
      841 tctataatgg acttttctaa aaaaatatta aaaattaacg aagtagagtt tatagcaata
      901 ccaacaacaa gtggaacagg ttcagaagta acatcatttg ctgtaataac agatagtcaa
      961 aaaaaatgta agtatccatt agtgagtgat gatttacttc cagatatggc aatactagac
     1021 ccagaacttg taaaaactgt accaaatttt ataacagcag atacagggat ggatgtattg
     1081 acacatgcta tagaagctta cgtatctaca aattctaatg acttttcaga tgcactagct
     1141 gaaaaagcta taaagttagt atttaaatac ttaataaaag catataaaga tggaaatgac
     1201 atagaagcta gagaaaaaat gcacaatgca tcttgtttag caggtatggc atttaaccaa
     1261 gcatcacttg gcataaatca ttctattgca catgtattag gtggaaaatt ccatatacca
     1321 catggaagaa caaactctat attacttcct catgtaatag aatataattc atcaatagta
     1381 ggatatacta acacaactta ctctgctaca gctaaaaaat atgcacaaat agcagaatta
     1441 gtaggacttg ggagtgctaa tataagaata tctgttagaa atttagtaaa tgaaataaag
     1501 aaactacaaa aagaaatgaa tatgccaact aaattgacac aatgcaaatt aagtttagaa
     1561 gatataacta atgaagaaag agaaatagca aaacttgcta taaaagatac ttgtacatta
     1621 actaatccta aggtaccaac tgaagatgat ataataaaca tattaaagaa cattaaataa
     1681 ctacatttta ttacatgtaa atataatatt atacaaatag ggaaaatgtt tttaaaaata
     1741 taaattatat attgacaaat ataaaacata aaactataat gaaagtatca caaaggcgtg
     1801 atactttagt agaaagcaat ggagcttctt aggaatatta atattattct tatggagctc
     1861 cttttatttt aatcaaatag ctttttaata aactatattc aaagaaagca gggaataaat
     1921 ttgactgaag aatctaagca aagagttatt caagaatatg ttcctggaaa acaggttaca
     1981 ttagcacata tcatagctaa ccctaatgaa gatatatata agaagttagg attagtactt
     2041 gataaaaaag atgcaatagg aatacttaca ataacaccaa gtgaagcgtc aataatagca
     2101 gcagatgttg ctacaaaggc ttcaaatgta tctttaggtt ttatagatag atttagtggt
     2161 tcagtagtaa tatcaggtga tgtaagttct gtagaatcag cgttaaatga tgttttagaa
     2221 gtcttaggaa atatgttaaa cttctcatct acaaaaatca caaggacata ataatacaat
     2281 gaagaatata atatttatgg gaaagacagg tagtggtaaa actaccttat gtcaaaaact
     2341 agaccagtta gaattaaagt ataaaaaaac tcaatcagtg gaactttata ataatgcaat
     2401 agacactcct ggtgagtata tggaaaatag aaacctatat aatgccttaa taacaacatc
     2461 agcagatgct aaaattatag caatagtgta tgatgccaca caagaagaaa attatatagc
     2521 accaggtttt gccagtatgt tttgcaaaga cgtaatagga attataacta aaataaataa
     2581 agctactgaa aacgaagtca atatagctta tgaaagacta tcactagcag gagcaagtca
     2641 aatctttaaa atagacacag ttgataatat tggacttgaa aaattattta aatatctaga
     2701 aaaatataaa aatatagatg aagtttaagg ggaaatattt ttttattcaa aaatattatt
     2761 aaagtatgat aaaataaaaa aatgtataaa ctattttaag aagatttaat ctttaagcga
     2821 ggtaggaaca tggggaaaaa aatactattg gttgatgatg aaccactgtt aaggatggat
     2881 acaaaagata tattagaaag tcatggctat gaaataatag gcgaagcaag tgatggattt
     2941 gaagctgttg aaatgtgtaa aaaacacaaa cctgatttag ttataatgga tatagagatg
     3001 ccaatacttg atggtataaa ggcaggcaaa ataatatgta aagaaaatat agctggtgga
     3061 gtacttttat taacttcatt tgaagataaa gaatacgtag aaaaggctaa aagtataggt
     3121 gcttatggat atttagtaaa aacagcaagt gaaaaatcac ttatcccaac catagaaatg
     3181 tgtttaaata aggtaaatga atttgaaaaa atgaaaaaag atttagaaaa agtaaattct
     3241 aaattgaatg aaagaaaatt agtagaaaga gcaaaaggta tacttataag agagtttaag
     3301 atttctgagg atgaagcata cactagaata agaaaattaa gtatggataa aagaactaca
     3361 atgatggaaa ttgccaaaat gattataata ggatacgatg actaatacga taaaaaaaat
     3421 atcaactgca caaacatact taaataataa cgaaattgaa aaaattatag aagtatcaaa
     3481 atccttagat ttaatggctt ctttttatga agctgatgtt tttatagatg tactaagtaa
     3541 atttgatgat gaagcaattg ttgtggcaca tggtagacct aaagaaaagt caatgtactg
     3601 tgaaaatgtt ataggtaaaa aggctttaat tgaaaatgaa ccaggagtta taaagtgctt
     3661 aaaaacagga gaaagagtaa gagatattaa agctttaaca caggaatata aactggtcaa
     3721 acaaaatatt cagccaataa cactagatga caaagtttta gcagtactaa tagttgaaaa
     3781 agatataagt agtgaagtta agaatgagtt taatgctagt gaagacgaag aaaataaaaa
     3841 tttacatcag tttataaaac ttattggaaa taatgacttt ttagttgata atttaaattc
     3901 agccatatta atatttgata aaaatggaaa attaaaaatt aaaaatcgta ttgcaactga
     3961 gatatacaaa aatcttgggt ttggatatga tatacaagat atgcattaca atgatttaga
     4021 tatagaaacc aacacctttg aaaatatatt aatggatgaa aattatgatg agacaaatga
     4081 agtttcaata ggtaatatgt tttttaaaat aaagaccata tacatcaaag atgatgaatt
     4141 taaggtttgc aaaatagtac aagatataag tgatttaaag agaaaagaag aagaattagt
     4201 tttgaaaaca gtggctataa gagaagctca tcatagagta aaaaataatc ttcatacagt
     4261 aatatctata ataaaaaaac aaagtagatt atctcaaaat gatgaagtaa agcagtgttt
     4321 agataacata ataaataggg tatttgcaat actttcgact cattatttac tatctaaaga
     4381 agttgataat aatatatcta tacgagaagg tatcaattta ttagtgtcaa atattcaagg
     4441 tggctattta tttagtaagg atatagatat acgtataact ggtgaagatt ttaaaattag
     4501 tggagaaaaa gcaacagcaa tacttttggt tataaatgaa atactacaaa attgttatga
     4561 ccatgcattt tcggatagag aacatggtac tatacaaata ttaataaata aagataacga
     4621 ctatagaaat attgctatta tagatgatgg aattggattt gatagtaata atataaataa
     4681 aaatagctta gggacatata taatagatag ttatataaca cagatgttaa aaggaaatat
     4741 agagagaaaa agtagtgaga atggtacaga agtattaatt actataccta cacaaattaa
     4801 tatttaaata ataactcatc tacaaagtag tagttttaat tgagcaaaga ggcttaaggt
     4861 tattaataga ttaagttaat tgaatgtatt tataaaaatt ataagtatat attcaattta
     4921 acaggtattt attataactt taagcctctt tgcttgttta agacatactt aaattttagg
     4981 aggtgagaaa atgagagaag agttattaag tgttggtatt gacattggaa caagtactac
     5041 tcagttaatt ttttctaaat taatagtaga aaatactgaa tcaaattttt cggtacctag
     5101 aataagtata gtagacaaag aaattattta taaaagcaaa gtttatttca cccctcttat
     5161 ttcaaataat gaaataaatg gtcaacaaat aaaagatata gttgaaaatg aatataaaaa
     5221 agcaaatata tctaaagaag atatacatac aggagcagta ataataactg gtgaaacagc
     5281 tagaaaagaa aatgcaaatg atgtacttca tactttgagt ggatttgctg gggacttcgt
     5341 tgttgcaaca gcaggacctg atttagaaag tataatatca ggaaaaggag ctggagctca
     5401 tatttattca aaagaacatt caactacaat tgtaaatata gacataggtg ggggaactag
     5461 taacatagcc tcatttaaaa ggggagaagt aaccgatact gggtgtttgg atataggtgg
     5521 aaggcttgta aagatagata aaataagtaa aaaaataact tatatatcac ctaaaataga
     5581 taaaatcata aaagataata atttaaatct tcaagtaggt acaatggcta ctgtagaaaa
     5641 tacaaagcca atagtggata taatggttga cttattaatg gaaagcatag gactaaagaa
     5701 acagacacct ttgtttgatt ttataataac caataaatca ataagtatag atgaaaatat
     5761 taaaaatgtt tcattctctg gtggagtagc tgattatatt tactatgatg gagaaataaa
     5821 agattatttt gaatatggtg acttgggaat tttacttggg caagcaataa aaaattgtga
     5881 ataccttaaa aaagtaaatg tagttaaatc tatggaaaca ataagagcca cagtggttgg
     5941 agcaggttct catactactg aagtaagtgg aagtacaata acttacacta gcgaaaagtt
     6001 tccacttaag aacttgccag ttttaaaatt atcaattgat gaagaaacag gtacttcaaa
     6061 acaaatcagt gaagctataa aaaataaact taattggttt aaactagaaa atgatttaca
     6121 gaaagttgct atagccatga taggaaagaa aaatccaacc tttttagata tacaagagta
     6181 tgctaaaggg tttgtagatg gaatgcaaca aatcatagaa aatgatttac cttttatagt
     6241 aattgttgaa aatgatatgg caaaagtgtt aggtcaagca atatatgcat tattaaatta
     6301 tgaaaaagaa attgtttgta tagacagtgt caaggttgaa aatggagact atatcgacat
     6361 tggaaaacct atagttgaag gtaaagtttt acctgtggta ataaaaacat tagtttttaa
     6421 ttaatagaag gaggtaagta aatgatttta aatacaaaat tatttggacg tacttatcag
     6481 tttaaaggct tatatgatgt aatggctaaa gctaatgaag aaaaatcagg agataaatta
     6541 gcaggtcttg cagcagaatc agctgaagaa agaatagcag caaaagttgt tttatcacac
     6601 ataaaattgg aggatataag aaataatcct gcagtggcat atgaagaaga tgaagtaaca
     6661 agaataatac aagatgctgt aaatgaaaaa gtttatgaag aaataaaaaa ttggacagtg
     6721 tctgaactta gagaatggct attggattca aatactacaa gtcttgatat aagaagaata
     6781 tcaagaggat taacttctga aatgatagca gcagttgcaa aattaatgtc aaatatggac
     6841 ttagtttatg cagctaaaaa gataaaggtt actgctcact gtaatacaac tataggagaa
     6901 caaggaacat tatcagcaag acttcaacct aatcatccaa cagacgaccc agatggaata
     6961 cttgcatcac ttcttgaagg tttaactttt ggtataggag atgcagtact tggattaaat
     7021 ccagttgatg actctgttga gagtgttact aaagtattaa aaagatttga agaagtaaaa
     7081 caaaaatatc aaataccaac tcaaacatgt gtattggcac acgttactac acaaatggaa
     7141 gcaataagaa atggtgctcc tgcagattta atattccaat ctatagcagg ttctgaaaaa
     7201 ggaaatgaag catttggatt taatggcgac actatagaag aagctagaca gttagcctta
     7261 aaacacggta cagctacagg acctaacgtt atgtattttg aaactggaca aggttcagaa
     7321 ttatcttcag aagcacatca tggaatagac caagttacta tggaagctag atgctatgga
     7381 tttgctaaaa agtatgaacc attcttagta aatacagttg ttgggtttat aggacctgag
     7441 tacttatatg atgctagaga agttgtaaga gcaggacttg aagaccactt tatgggtaaa
     7501 ctacatggct taccaatggg tgttgatgtt tgttacacta accatatgaa ggctgaccaa
     7561 aatgatatgg aaaacttagc catactatta acaacagcag gatgtacgta ttttatggga
     7621 atacctcatg gagatgatgt tatgcttaac tatcaaacaa caggatacca tgaaacagca
     7681 tcacttagag aaatgtttgg actaacagca ataaaagaat ttgatgcttg gatggaaaag
     7741 atgggattct ctaaagatgg taagttaaca gagttggcgg gagacgcatc tgtattatta
     7801 ggataggagg tatttaatat gaatgagaag gatttaaaag cattggtaga acaattagtt
     7861 ggacaaatgc ttggagaatt agatactaat gttgtgtctg agactgttaa aaaggcaact
     7921 gaagttgtag tagacaacaa tgcttgtata gatgatataa cagaagttga tataagaaag
     7981 caattactag taaaaaatcc taaggatgca gaagcttatt tagatatgaa agcaaaaaca
     8041 cctgcaagat taggtatagg aagagctggt actagatata aaacagaaac agttttaaga
     8101 tttagagcag accatgctgc tgcacaagat gctgtattct cttatgtaga tgaagaattt
     8161 ataaaagaaa ataatatgtt tgcagtggaa actttatgta aagataaaga tgaatactta
     8221 acaagaccag atttgggaag aaaattttct ccagaaacta taaataatat aaaatctaaa
     8281 tttggtacaa atcaaaaggt tttaatatta gttggagatg ggcttagctc agctgcaata
     8341 gaggcaaatt taaaagactg tgtgccagct ataaaacagg gtctaaaaat gtatggtata
     8401 gattcaagtg aaattttatt tgttaaacat tgtagagttg gtgctatgga ccacttaggt
     8461 gaagaattag gttgtgaagt tatatgtatg ttggttggag aaagaccagg attagttact
     8521 gctgaatcaa tgtcagcata tatagcttat aagccatata taggaatggc agaagcaaaa
     8581 agaacagtta tatcaaacat tcacaaaggt ggaacaactg cagttgaagc aggtgctcat
     8641 atagctgaac ttataaaaac tatgttagat aaaaaagctt caggaataga ccttaaataa
     8701 agtcgaggga gtgtagaata tgaaaaatga tgtaattcgt cctaatatac taggaattaa
     8761 aataatttca aatataagtc cagaaatggc acaaaaacta gaattaaaat cacatcacaa
     8821 aagcttaggt ttaataactg cagactgtga tgatgttacg tatacagctc ttgacgaagc
     8881 aactaaagct tcagaagttg atgttgtata tgcaaaatca atgtatgcag gtgctgcaaa
     8941 tgcttcaaca aaacttgctg gtgaagtatt aggaataata gcaggtccaa gcccagcaga
     9001 agtaagaagt ggtctaaatg cagtagttga ttttctagag tatggagcaa cttttataag
     9061 tgcaaatgat gatgattcaa tagcatacta tgctcactgc gtatcaagaa caggtacata
     9121 cctttcagag gtagcaggaa taagagaagg tgaagcatta gcttatttag tagcaccacc
     9181 acttgaagca atgtatgcac tagatgcagc aatgaaagct gcagatgtta agatgtgtga
     9241 attatttgca cctccaactg aaacaaactt tggtggagca ttacttacag gttctcaatc
     9301 ggcttgtaaa gcagcatgtg atgcatttgc agaagcagta aaatcagttg cagacaatcc
     9361 aacaggattt taaggatagt tagatggatg ctataggctt aatagaagta ataggatatg
     9421 ttgctgcaat agaagcttct gatgcttgtt taaaatctgc aaatgttaat attgtaagag
     9481 ttgataaggt tggggcaggt attgttactt taacaatatg tggagatgtc ggtgctgtaa
     9541 aagctgcttt agaagcgggt gaaatagcag cttccagagt tggtacatta agaacatcac
     9601 atattatacc gagaatccat aatgaagtaa caaatgcttt attcaaaact aaagaaccta
     9661 aagtctgtga agtttcagaa gataaagaaa aaaactttga gattacattg gaaatagctg
     9721 ataatgtatc gaaaaattta tcagaaaaag aaaatgtaat tttagatgtc aatgaaaaca
     9781 gtggagatac tgaaaattta tatagtgata tagataaaac aaacaaaaaa attgaaacag
     9841 ttgatgatag tttaacagaa gctaatgagg aaaaaataga aaacactaat gataaagaag
     9901 tattggaaca agttaaaata gaaaattccg attttgatag agatgaccta tcaaagaaca
     9961 gtgtaaaaaa acttaaagct atggctaaaa aattagattc ttcaataact tataaagact
    10021 taaatgttct taaaaaagaa gaattaataa acttagttaa aaagcttagc agaagggata
    10081 gatagttatg acacttttag ataaagattt agtatctata caagaagtaa gaactcttgt
    10141 aagaagatct aaagtggctc aagagaaact ttctaaaatg agtcaagagc aaatagataa
    10201 aatagtaaaa gctgttgtag atgcagcatt taataatgct actaaattag ctaaaatggc
    10261 aaatgaagaa actggatttg gaaaatggga agataaagta attaaaaata cttttgcatc
    10321 tatgggagtt tatgaaactt taaagaacac aaaaactatt ggaataattg gtgaagataa
    10381 agaagccaat acgatagata tagctacacc agttggagtt gttgcaggtc ttataccttc
    10441 aacaaaccca acatcaacag taatatacaa aactataata tctctaaaag caggaaatac
    10501 tatagttttc tctccacatc caaatgcaaa aaactgcata cttgaaacag ttaatataat
    10561 aagtgaggct gctataaagg ctggagctcc agaggattca atattatgta taactaatcc
    10621 aagtatagtt ggaaccaatg aattgatgaa acataaagat acaaacttaa tactagcaac
    10681 tggaggagaa gcaatggtta gagctgctta ctcttcagga acaccagcta ttggagttgg
    10741 accaggaaat ggaccagcat ttatagataa atcagcaaat gttaaattag caatcaaaag
    10801 aataatagat tctaaaactt ttgataatgg tgttatatgt gcatcagaac aatctatagt
    10861 aatagaaaaa tctatggaag aaactgtttt aaaagagctt aaaaatcaag gtgcatatgt
    10921 tttaaatgaa gaggaaagtg aaagattagc aaaattcata ttaagagcta atggtactat
    10981 gaatccacaa atagttggta aaagtgttga ggtaatagca aatttagcag acttaacaaa
    11041 tgtacctaaa aatacaaaag ttctagttgc aagagaaaat aaagttggtc acaatgtagc
    11101 ctattcaaga gaaaaactta caccaatact agcgttctat gttgaagaaa atgtagaagc
    11161 tattatgaac agatgcagag aaatattgtt aaatgaaggt gcaggtcata cattctcaat
    11221 gcatgtaaat gatgaagaat tggtaaaaag atttgccctt aatatgccag tatcaagaat
    11281 acttgtgaat actccttcag cacttggagg aataggtgca tcaacaaacc ttgtaccagc
    11341 actaacactt ggttgtggtg caataggtgg aagttcaact tcaaacaata taggaccact
    11401 tgatttaata aatgttaaaa gagtagctta tggaatcaaa gaaatagaag aacttggaag
    11461 agataaatct caagttgaaa aatcaatgtt tgactcaatg gataaagaag aattaataaa
    11521 tcttcttgtt caaaaactag tcaaagaact agcataacta aaataaacaa tgaaacttat
    11581 ggctatcgct ataaatttct aaaaatataa actataaaaa actataaaat taaatttttg
    11641 gaggataaga ttatggcaag tgcaaacgca ttaggaatga tagaaactaa aggattagta
    11701 ggagcaatag aagcagcaga tgcaatggtt aaagctgcaa atgtacaatt agtaggaaaa
    11761 gagcaagtag gtggaggatt agttactgta atggtaagag gagatgttgg agctgttaaa
    11821 gctgctacag atgcaggagc tacagctgcc gagagagttg gagaactaat atcagttcat
    11881 gttataccaa gaccacactt tgaagtagat gctatattac caaaggtatc agctgagtaa
    11941 tataagaata attataaaat ttaaattttg attgaaaaag aggctatctc aaaaatgagt
    12001 cttactaata aactcaatgt attttgaggt agtctttttt atacatctaa taggagatgt
    12061 gataaaatgg gtgttttaac agaaaacgaa gtaaaaagaa aaattggaaa aaacaaaaat
    12121 aatataactg aattttatat agaaaaaggt caaataataa ctccttctgc aaaatcttat
    12181 ttagctgaga gaagtattga gcttaaatat gttgataaac tttcagatgt aaacaatgaa
    12241 cctaaagaag ttgtaaaaga agtagttaaa gagaaaaaat ataaatatac tactgtattt
    12301 ggcgccaaat tggatgaaaa accagaacat atgactcaat taaatggaaa tcttttagtt
    12361 tttaaagacc ataaaagaat aatattgaga ggaaaaatag attctcttga atcagaaata
    12421 ctaaaggtac agatactttg tcagaaacta gatatgccaa agcttgttaa ggaattgcaa
    12481 gaagtattag actttgtaag aaatataatg agatgtgaag ttttagatga aaagctaggg
    12541 gactttaaac ttttaggatt aactcctcaa gaacttagag aacaatctca ctatcctaaa
    12601 aaatactttg gtataggaca tgagttccca agttatgaaa tgggagaagt agtagtagct
    12661 ataaatagta taagaagtaa tacacgagag gttgaacttg cagcctatga agcatttaag
    12721 ggtgagtatg gaaatgttca cagagaagat ttaataaagg cacttaatag attatcgtca
    12781 gtattttgga taatgatata taaggttaga actaataagt ataagtagtt ggaggacata
    12841 atggagaatc tattaaatga aatagtggaa gaagtaataa ctagagttaa aaaagaagca
    12901 tttatagaag ttgaagcatc aggaagacac gttcacttaa gtagagaaga tgtcgataaa
    12961 ttatttggag aaggatatac tcttacaaaa ctaaaagacc tatctcaacc aggtcaatat
    13021 gcatgtaagg aaagagttac aataactggt cctaaaggaa gtataaaaaa tgtagttgta
    13081 cttggaccat gtagaaatga aactcaggta gaaatatctt taacagatgg ttcaactctt
    13141 ggactaaaag ctcctataaa acaaagtgga gacttagaag gtactttaag tattaaaata
    13201 tcaacacaat atggtgaagt tgaacttgat aaggggctaa tggttgctaa aagacatata
    13261 cacatgactc ctaaagatgc tgaaaaattc aatgtatgtg ataaagagat agttgaagct
    13321 aaggtgatgg gtcaaagacc tttaacattt gatgatgtag taataagagt gagtgacagt
    13381 tttaaaactt atatgcatat agactatgat gaagccaatg catgtggtta ttcaaaagga
    13441 actgttgcta aaataataaa gaaagcataa atttaaccta ctatataaat tacctctagg
    13501 gaggatttaa tatgaattat gataaccttg tagatttaat aacggaagaa atctataaaa
    13561 aaataaatag taatgaaata aaaatatcta ataaacctaa agctgtgatt gtatttgaac
    13621 aagataacaa taaatttaac ttattaaaag atggatttga aatagtagaa tttgataaaa
    13681 gtataaggga atgtgaaata gtgatagttt caagactttg tatgaggggg ctatcgaata
    13741 tagcacttgg aaattcaaca agtgatgaag aaagatttat actaaaaatg attatgaatg
    13801 gcaaaaaagt atatgtccta gatgaaggaa ttgaatacaa aaaatataag gatactgcac
    13861 caaaagcact atataagaaa tttatgtcat ttgaagatga aatatgtaaa tttggtgtag
    13921 aaattataaa agacttaaat gttataacta aaaataagtt aaatataaag aaagaactag
    13981 aaagtactag aggttctaaa aaagaagata caaaagtttt agataaagat attttaaatt
    14041 taaccagtgt agaaagtgta aaaatagata atgaatttag tttggattta aggaataaaa
    14101 aacttatatc ggaagctgac ttaagaaaac caactataaa tggagttaaa aacatattag
    14161 ttaataaaaa aagtataata acaccattag ctgtagattt tatgagaata caccatctca
    14221 aactaaagaa actgtaagga ggtaatttta tggatatagg aaaagttgta ggtaatgttt
    14281 gggctacaag aaaagatgaa aaattatgtg gccaaaaact tttagttgta aaaattttaa
    14341 aaactaaaga cacatacaag gaaggcttca tggttgcagc cgataatgca ggagcaggaa
    14401 atggagactt agttcttata tccaaaggtt cctcagctag agagtcgatt gaaaaatctc
    14461 atgttccagt agatgcaact atagttggag tagtcgattc gtttgaggtg tttgatgaat
    14521 aaaagtatag gagctataga gtttaaaagc attgctaaag gtatagaagt atccaatgaa
    14581 atgataaaga aatcttcagt tgatgtattg tacttaaaaa gtatttgccc aggtaaattt
    14641 ctaataatag taggtggaga aacttcttat ataaatgaat gtgtagatta tggaataaaa
    14701 cttggagaag gatatattgt agataacttc gtaataaatg caatttcaca agaaatatta
    14761 gatggattta aaaataagta tcaaaagcta gatagtatag tctctatagg cgttgttgaa
    14821 agtagtaaaa tatgcgctgg aataaaaatg cttgataaga ctttaaaatc tggcgactta
    14881 gtattagtaa aacttcaatt atcttttgct ataggtggca aattagtata tatagtagca
    14941 ggagatttaa gtagtttaga atatgcatta aaagaaagtg aaaatgtagt aagagaaaaa
    15001 gaagtaatat ataagactgt aataccatca gtagacagtc aaataataaa aagtctaata
    15061 aaataatggg gggaattatg agtataaacg aaattataat ctatgtaatg gtagtcttca
    15121 tggtacttgg agctatagat aaatgtatag gtaacaaatt tggtcttgga gaacaatttg
    15181 aggaaggtat aatggcaatg gggtcacttg cagttgcaat ggttggtgtt atatgtttag
    15241 caccagttct tgctgatgta ttaagaccaa ttgtagtacc agtatttaat atacttggag
    15301 ctgacccagc tatgtttgct ggaagtttat tagctaatga tatgggtggt gcgccacttg
    15361 cacttgaact tgcgaaagac cctaatgctg ggttatttgg tggattaata gtaggggcta
    15421 tgatgggacc tacaatagta tttataatac cagttgcgct tggaataatt gaaaaagaag
    15481 accaaaaatt cttagcaaca ggaatacttg caggtataat aacaatacct attggagcat
    15541 tcgttggtgg attagttgca ggattcgagg taatgatggt tcttagaaac ttagtgccaa
    15601 taataatatt tgcagtagtt atagcacttg gattattaaa attcgaaaat gctatgataa
    15661 aaggattcac atactttgga aaaggtgtag taatagttat aactttagga cttgctgctg
    15721 ccatagttga agctttaaca ggaatagttg taatacctgg aatgaaacca atcagtgaag
    15781 gtatagaaat agttggagat atagcaatag ttttagcagg agcattccca ttagtgtttg
    15841 ttataacaaa ggtatttaat aaaccattaa tggctttagg aaaagttctt ggaatgaatg
    15901 atgtatcagc agcaggcctt gttgcaagtt tagctaactg tataccaatg tttggtatga
    15961 tgaaagatat ggacaataga ggaaaaataa taaatgtagc attctctgta tcagcagcat
    16021 tcgtatttgg agaccactta ggatttacgg caggatttaa ttctaatatg ataacaccaa
    16081 tgatagttgc taaattagtt ggtggtataa cagcagtatt gttagctatg gttatcgcaa
    16141 ataagacact aaagaaggag aatgtataag atggatatat caaatataga taaaaattta
    16201 attgaaacat tagtaagaca aataatagaa gaaaaaataa gtggaactaa agacacagta
    16261 gattttgtta gaaataaaga cattagtgga ataacttcta taaagttacc aactgtaaaa
    16321 gttagtgaat ctgatagatt agatacaggg aatccaagtg atgtagttta cacaaaagat
    16381 ttatttacat tagaggaaag cccaagatta ggttgtggta tgatggaaat gaaagaaaca
    16441 acttttgatt ggactctaaa ttatgatgaa atagattatg taatagatgg aactttagat
    16501 ataataatag atggaagaaa ggtttctgct tcttcaggtg aattaatatt cataccaaaa
    16561 ggaagtaaga tacaattttc tgtacctgat tatgctagat ttatatatgt aacatatccg
    16621 gcagattggg catctcaaaa ttaataaaaa tcaagttaag ttaataatca aataaataat
    16681 ttaaaataca aactcttaaa taagcacgca tatttaaaaa agctatgaat taaaattcat
    16741 agcttttttt ataaagaatg ttatatttta gacgtatttt ttctatattt actgggagat
    16801 atattatatt ttttattaaa taatctaata aaatgctcta catttggata accaatacta
    16861 ttttactgat aatcttttcc ttatcactaa tcttacattt taaaatatta tttttactcg
    16921 taatattatc ataagaattt gtttcttatt taaaactaat tagaattata aagtttattg
    16981 ccatgttatt aataaaataa atccagttaa atcgagtgct agttaaagta ataagaaact
    17041 tttacaatgg tttcctttat cgattgaaat aaaagttaat atggtataat atctaaagag
    17101 agaatcttat caattagaat atgcatagaa acaagtggct taaaaggaga cgttatatgg
    17161 aagagatagt aaagaaggaa caagcaagta tgaatgatat ttttttgtat cttaaagcta
    17221 aatcaaactc attatataaa tttgtaatat tatataatga gtttatcaat aaacctaaag
    17281 attatggaac tggggaactt ataaacatgg ttgaagtaca tatgctaaca gcgattgaag
    17341 aaaatccagg aataagtcca actcagttag ctaaaatatg gaacagaact aaaggtgcaa
    17401 tatctcaaac tatatctaaa ttggagaaaa agggttatat aactaaagaa aagaaagaag
    17461 gaaatgcaaa aactatatta ctatatccaa ccacatcagg agtagagtta agtaaggcac
    17521 ataaactata tgacaccata gatgttacaa gaacatttga atacttaatc aaggagtgta
    17581 cattagagga gatagatagt ttttataaag taattgattt gtttattaat ttattagaag
    17641 aatctccgca ggaaaataat gattctgaaa aagtgtagct gagattgaag ttgaataaag
    17701 agaaatagat attatagaag tagtacttaa gtacacatta tataaaaata taataggtat
    17761 aggattaaaa agtttagtgg tttaaagttt aaccactaaa cttttttaat gtctataatc
    17821 tactattatt taatttacta aaaataaaaa aactcattat attaagatag aaatagaaaa
    17881 ataactagaa aaatactata atatatcagc ttaatacaga aaaaagttaa aaaatttgag
    17941 aaaaataaaa atgaaaacgt tttatattga catagagaaa aagtagtgat accatttaaa
    18001 tatagtttag tagctaaact atatttaaat tataataact attttagggg gatgttatga
    18061 atttaaaaca attaagtgaa aaatacgaag aatatgtaat tcaaatgaga agagaatttc
    18121 ataagtatcc agaattatct tttaaagaaa taagaacaac ccaaagaata agggaagaat
    18181 tacaaaaaat aggggtccct tttgtatcta ttgaaccaaa tatcgtaata gctactatac
    18241 aaggcaaaaa tgaaggtaag acaattgcaa ttagaggaga tatagatgcg cttccaatgc
    18301 aagaagattc agatgttgaa tacaaatcag aatatgatgg aataatgcat tcttgtggac
    18361 atgatgctca tggagctatg ttactaggac ttgcacatat gctaaatgat ataagaaaag
    18421 atttaaaagg tagagtatta ttagtttttc aagcagctga agaagttggt ggtggacaca
    18481 aggagataat acagtacttt aaggaaaatg gaggaccgga tagattaata gctactcatg
    18541 tttggtccga tattgaagct ggtaaaattt ctgttgaacc aggacctcgt atggctggtg
    18601 ggtctggttg gagaatagac ataacaggta gaggaggtcc agatcaaagc atagatccta
    18661 taaagccagc ttgtgatata gttttaaaag tatcttctat accagttaat catataagtg
    18721 tactagaaca atgtgttgta catgcatgtg caatacatgg aggaactaca ataggaaatg
    18781 ttattccaaa caatgcagag gtaataggtg gatatagata ttttactgaa gaatcaggca
    18841 aaaaagtgtt tgacattata aagcaaatga gtaatggtgt tggacaagtt tatggagtgg
    18901 atgtgaaggt tactcatact ggaggcatag gacctgttat caatgatgaa gaagcagtta
    18961 agatggctaa agaaatagtt tctaatatag atggattgga gttagattct tatgagccaa
    19021 tatgtgcatc agattgttat ggtgaaatat taaaagagta ttctggcttt tattgttatc
    19081 taggggtagg taacaaagaa aaaggaataa tatatgcaca acaccatcct aagtacaata
    19141 tagatgaaga tatattaaag ttaggttgcg aatttatggc taaatatgca gttgaatttt
    19201 taaataaaaa taataactaa aataaatcta attttataaa tggggggatg aaaatgcaag
    19261 gtgcattaaa ggaaaataag aaaaaaagtg gtggaggatt tttaggatgg attgaaaaag
    19321 ttgggaataa aatgccacat cctctagcat tatttacata tattacactg atagtagtag
    19381 cattatctgc aatagcaagc tttttaggtt tttcagcaac aagtcctgct acaggaaagt
    19441 taataaaagt tatgaactta ataagtgcaa aaggtttagt actatttatg caagagtttg
    19501 ttaagaactt ccaaaacttc cctgtgcttg gagtagtttt agttatggca gttgccacag
    19561 gagtttgtga aaagacagga tttttttcaa ctgccataaa aatgagttta tcaaaagtta
    19621 aggggaatgc ggttgtattt ataatagctt ttataggagt atttgctaat caagctggtg
    19681 atgctgcttt tgtattagta ccaactcttg caggagcaat attttatgga ttaaatagaa
    19741 atccattagc tggtatattc tgtggatttg catcagttgg tggtggattt gcaacagctg
    19801 taattcctgg gggatgggac gttacgctaa cgccaataac tgtatcagct gctcaaacta
    19861 ttcaacctgc atttgatatg acattattag ctagttatta tgcactattt gtatctgcgt
    19921 ttatagtagc tacagtatca acaatagtaa cggttaaatt tatagaacct aaattaggta
    19981 agtatactgg taaaccagaa ggtatagatg ataatcaagg ttttgaggtc acaaaagaac
    20041 aagcaaaagc agcaaagtta gcaggtttta cagttttagc ttatgtagcg ttattaattg
    20101 ctttgtgtat accagctaat agttttttaa gaagtccaga aggttcttta atatttggag
    20161 caccacttat gtcttgctta cagtttttta tagtaatact attcttcttg ccagggattg
    20221 tgtatggaat gagggttggt aaaatcaaga ctgtaaaaga cttaaatgat atattatgtc
    20281 aatcaatggc agcaatggca ccgtttatcg tacttgcaat agttataggt caattcttaa
    20341 cgctattctc agtgtctaat ttagcacagg tactagctat aaaaggtgga gcattactca
    20401 actcattgcc actaccacca caagctataa tactgctatt cttagtacta gtagcgttta
    20461 taaatatatt tgttataagc ggaagtacaa aatggatgat atttggacca gtatttgttc
    20521 caatgttaat gcaattaaac atacatccag catttactca gtttgtatat agattaggag
    20581 attctgtaac caatcattta tcaccattaa atgcattctt tataatactt ttagcaacag
    20641 tccaaaaata cgataaaaat gcaggtatgg gaactatatt ctcagctatg ataccttaca
    20701 ctataggtta ctttgtagta cttgcagttt tagtagtagt ttggatgaca ataggtatac
    20761 cagtaggaat aggtggacaa gtttggttat aaaacatatt taataatttt gggaggaaaa
    20821 aaaatggaaa caactgaaat aagatacaat tttgatgaag taatagatag aagtggaaca
    20881 aactgtctta aatatgatct taagagtcaa tttatgccag aatctccaga agatgcacta
    20941 ccactatggg tagcagatat ggattttgca tgtgcccctg agataattaa tgcaatgcat
    21001 gaaagaatag acagaaaaat atttggatat agctctcaac aaactgatga ttattatgaa
    21061 gctgtatgtg gatggtttaa gagaagattt gattggaaaa tagaaaaaga gaatatattc
    21121 tttagcccag gggttgtgcc tgcgttagga tttttcacac aaatattaac acaaaagggt
    21181 gatggtatca taatagaaaa gcctgtatac tatccttttg aagataaaat aactaacaat
    21241 gaaagaaaag tagttaatag tcctttaaag tttgaagatg gtcattattc tatggattat
    21301 gatggatttg aagaattagc taaagatgaa aataataagg tgtttatatt atgtagtcct
    21361 cataatccag ttggtagagt atggaaaaaa gaagagctta gaaaaatagt agacatatgt
    21421 aagaaatatg atttgtggat aatatctgat gaaatacatt gtgatattat tcgtaaaggt
    21481 gaaaagcata taccattaga agtagcatgt cctgattata aagatagaat aataacttgt
    21541 acagctccaa gtaagagttt taacttagct ggtatgcaat tatcaaatat agtaatcaat
    21601 aataaggaat tacaagaaaa atggatgctt gaaacagatt ctaaattagc tatattccta
    21661 cctaatccat tcgcaatagt tgctactaaa gttgcttata atgattgtga agattggatg
    21721 aatcaagtaa atgagtatat agatggtaat attaaatatg tagaagattt tgttaatgag
    21781 tatatgccta aagtaaagat aataaaatct caaggaactt acttagtttg gttagacttt
    21841 agagggtatg gattagatgc aaaacagctt gaatatgtaa tgcttaacaa ggcgaaggta
    21901 ttgctagatg aaggatacat attcggagaa gaaggagcag gatttgaaag aataaatgtt
    21961 gcttcaccta gaacaattat agaagagtgt atgaatagaa taaaatgtgc atttgaagga
    22021 ttataatagt aatatctaaa taatatattt gtgtatttta aaaaaagact atgctaaaaa
    22081 aatatttcaa agcatagtct tttaaagttt tatgatgagt ttaaaaaatt tcaaaacgtt
    22141 actattcact tatatgttta tattagaaat aattccacaa tatactcatt ttctaaagtt
    22201 gcacttttat acaccttgct tcaccaatat catgtgtaac caaaatagta gacttattct
    22261 cattcttaat aatactataa acatcatcac aaactaataa tctagtctga taatcaagag
    22321 ctgaaaaagg ttcatctaaa agtagaatat ctggatttac agaaagtgtt ctaataagcg
    22381 caactctttg tctcatacct ccagataatt cttttggata catacttcta aaatcccata
    22441 atccataagt ctttaaaagg ctttcaactc tatctaaaga ttttttatct tttttgcctt
    22501 gaatttccaa tccaatagtt atattatcca ttatatttct ccattcaagc aaattatctt
    22561 tttgaaacat atatccaata tttccagtaa tctttatatc accactagtt ggttttaaaa
    22621 gattagtaag aatatttaat attgtagatt taccacttcc agaaggacca agcaaagtta
    22681 atatttctcc ttctttcaag ttaaaattga catcctttaa tacttcaagc tcgccttcct
    22741 tagtgtagaa gttcatattt atatttttaa cttctactat gttgtcattc attatgccac
    22801 cccctataac ttttagctcg tatatattat gttattaaca atatgaccta tttgtaccat
    22861 ttttcataaa tataatataa atattttgtt tatttacgta ttttcttagt aacttgtacg
    22921 tataaattat agataaataa attataaggt aggggataaa tttgagtaac ataataacga
    22981 atttaataga gtcaatattt actaatccaa taactatatt gtttttactt gcaataactg
    23041 tgtattctgt ttttaaaata atagaaaata acagagtata ttcatttgta agttcaaaat
    23101 tagacgaaat caatagagac tataagacaa gtgagtttta taaaaaggta agggatgact
    23161 actatacata tagtaaagaa aatccttatg cagacgtaaa tataacttca tttatagagg
    23221 aagttgtatc tgatttaaag cataataatt tgccattgtt agaaaaaatt agaagtataa
    23281 aaaattcatc ttcaataagt atattacttg gggttttagg tacgtttgtt ggtctttcta
    23341 caatgctttt atgtgttgat actaaagaca ttataaattc acttccttca actattagta
    23401 gtatgcaaac tgcatttaca actagtatat ttggagttgt attttctcta attataggtt
    23461 attttactaa gataaaagat
//
LOCUS       DACULQ010000047        22782 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.047, whole genome shotgun sequence.
ACCESSION   DACULQ010000047 DACULQ010000000
VERSION     DACULQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.047"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            188..652
                     /locus_tag="JA154_07450"
     CDS             188..652
                     /locus_tag="JA154_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAT7995701.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            659..1153
                     /locus_tag="JA154_07455"
     CDS             659..1153
                     /locus_tag="JA154_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAT7995702.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1169..2194
                     /locus_tag="JA154_07460"
     CDS             1169..2194
                     /locus_tag="JA154_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HAT7995703.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2181..4628
                     /locus_tag="JA154_07465"
     CDS             2181..4628
                     /locus_tag="JA154_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpC"
                     /protein_id="HAT7995704.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4808..6181
                     /locus_tag="JA154_07470"
     CDS             4808..6181
                     /locus_tag="JA154_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAT7995705.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6184..7254
                     /locus_tag="JA154_07475"
     CDS             6184..7254
                     /locus_tag="JA154_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086491.1"
                     /note="non-specific DNA-binding; scans chromosomes during
                     sporulation for DNA-damage; delays initiation of
                     sporulation; participates in a checkpoint signaling
                     cascade for cell-cycle progression and DNA repair; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HAT7995706.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7343..8437
                     /locus_tag="JA154_07480"
     CDS             7343..8437
                     /locus_tag="JA154_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAT7995707.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8566..10743
                     /locus_tag="JA154_07485"
     CDS             8566..10743
                     /locus_tag="JA154_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995708.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KETYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TIYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWYIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(10929..11774)
                     /locus_tag="JA154_07490"
     CDS             complement(10929..11774)
                     /locus_tag="JA154_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7995709.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            11991..13157
                     /locus_tag="JA154_07495"
     CDS             11991..13157
                     /locus_tag="JA154_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HAT7995710.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYRVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYVCGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            13179..14825
                     /locus_tag="JA154_07500"
     CDS             13179..14825
                     /locus_tag="JA154_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAT7995711.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            14922..16295
                     /locus_tag="JA154_07505"
     CDS             14922..16295
                     /locus_tag="JA154_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7995712.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCDTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            16740..17693
                     /locus_tag="JA154_07510"
     CDS             16740..17693
                     /locus_tag="JA154_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAT7995713.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            17725..18693
                     /locus_tag="JA154_07515"
     CDS             17725..18693
                     /locus_tag="JA154_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase"
                     /protein_id="HAT7995714.1"
                     /translation="MYMSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSV
                     GQEASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSM
                     HIADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGIN
                     LSSIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYET
                     VQKAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLI
                     ENNLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            18718..19704
                     /locus_tag="JA154_07520"
     CDS             18718..19704
                     /locus_tag="JA154_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAT7995715.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            19717..20763
                     /locus_tag="JA154_07525"
     CDS             19717..20763
                     /locus_tag="JA154_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin dehydrogenase"
                     /protein_id="HAT7995716.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            20930..22660
                     /locus_tag="JA154_07530"
     CDS             20930..22660
                     /locus_tag="JA154_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAT7995717.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
BASE COUNT         9242 a         2375 c         4306 g         6859 t
ORIGIN      
        1 aaaaattagc atttggttat caataaatat aataaataaa aactcataat taatgaaaga
       61 ataaattcat gtctaaattt ctataaaaaa tttaaattta tacaatttag ggctttaaaa
      121 atttaaataa taggtgtaaa ataatagtca aagaaagtca aaaataaaaa ggtgtgtggt
      181 gatattaatg gcaactatga cagatataat tgaaaaattt attaaagatt tgatggaaga
      241 agataatagc atacaaattc aaagaaatga actagcaaac ttgtttagtt gtgcaccatc
      301 acaaataaat tatgtcttaa caacaagatt tacaatagat agaggatatt acattgaaag
      361 taaaaaaggt ggcggaggat atgttcaaat agaaaaaata cgaaaaagta aagatgggca
      421 tattagagaa ctactaaatg aaaaaatagg aagtcagata tcctataaaa aagcaaaaga
      481 attattagaa ggtttaaaag agtcggattt gattaacgaa agagaattaa aattaatatt
      541 atatgcaata gatgataaat cattgtgtat gccaatatat gaattaaaag agaaggtaag
      601 gtctaatatt ttaaagaata taataatagg attatttagt atagaggagt gataatttat
      661 gctatgtcaa aaatgcaata agaataaagc atctgtatat tataataaga tagtcaatgg
      721 agaaaaaaca gagatgtatt tatgtagtga gtgtgcaaaa gagaatacag aaatgaattt
      781 taacttagat atgccttttt caatgatgga tatattttct aatttaggtt ttcaacctaa
      841 aaaagaactt gaggaaaagc tagtttgtcc taaatgcaat acaacatata gtgagtttaa
      901 gaataatggg agatttggat gtagtgaatg ttataatgca tttagtagtc aagtaaatcc
      961 aatgcttcaa aatatacatg ggcatataga acatacagga aaagcaccta aaaaatcttt
     1021 ttataagata agtgtagaaa atgagattaa agaactaaaa gaagatttag atagagctat
     1081 aaaaaatgaa gagtatgaat tagcagctca atttagagat aaaattaaat atttaaaagg
     1141 ttctattgac taagattgga gtgatattat gaaagagaac atagtcatga aatcaagagt
     1201 taggctagct agaaatttaa ataactatcc atttccaaat aaattagata aagaatgtgc
     1261 catggaaata atagaaaaag taaaaaatgc gtttattaat tctaacttag aacaaaaaga
     1321 agaatttgat ttctataaaa ttgaagattt agaccaatct aaaaaaatgc ttatggttga
     1381 agaacatata ataagcccag atttagcaga aaatgataaa agtgcagtta tagtaaaaaa
     1441 agataaaact ataagtataa tgataaatga agaagaccat ataaggatac aaactatatg
     1501 tgatgattta aacttagaat atgcatatag tgtagcaaat gaaatagatg atttgttaga
     1561 atcaagttta gaatatgcct ttaacacaaa gctaggttat ctaacatctt gtccaactaa
     1621 tacaggaaca ggaatgagag cttctgtaat gatgcattta ccagcattaa gtcaactagg
     1681 ctatatggat gaactttata aaatttcatc acaaataggt atagcaatta gagggatata
     1741 tggagaacga acagaagcat tgggaaatat atatcaaata tctaatcaac ttacacttgg
     1801 aagaacagag tctaatatta tagaaaatgt aagtggactt acaaaagatg caatttcaaa
     1861 agaaataaaa gctagagaaa tattgcaaaa aaaattaggt ataaaactag aagataaaat
     1921 atttagatcg atagggacac ttgaaaattc aagagtaatg agtagcgcag aggcaatgag
     1981 ccatctatca aatataaaaa tgggaataga aatgaactat atagataaac tagatttaag
     2041 ggctatagaa caacttatga taggtataca accagcgcat cagtcaataa tgtataagag
     2101 tgatgatgtt gagaatagag atataaatag agcaacatat attagagaaa ctttggaaaa
     2161 attaagagga gggatgaatt gtgaacttca atagatttac acaaagagct aaaaaggcaa
     2221 ttgatttagc gtttgaatct gctaaaagtc taggacacaa tattgtcgga agtgaacata
     2281 tacttttagg tcttttaaga gaagaagaag gtatagctgc taaagttcta agtaaagttg
     2341 gatttacaga agcttattta gaaggcaaaa tagttgatat ggaaggtaag ggagaagaaa
     2401 tctcagaaga tatagtatta agtccaagaa gtaaacaaat acttgaatta tcaggaatgt
     2461 ttgcaaataa attaaaaaca aattatattg ggactgagca tatattatta gcaattattc
     2521 aagaaggtga aggaatagct aataagattt taaattatgc tggagtaaat gataggactt
     2581 tagcccaatt aacaattgat atgatgggta ttagtgataa aaatcaatac aaagcagaaa
     2641 atagttacac gggcaaccag aatcaagcag aatctaaagt acttgataag tatggaagaa
     2701 atcttacact atatgcaaaa cagaataaaa tagaccctgt aataggtaga gaaaaagaaa
     2761 tacaaagagt aatacaaata ttaagtagaa gaactaaaaa caatccagtg ttgataggag
     2821 acccaggtgt agggaaaaca gccatagctg aaggtctggc tacaaatata gccttaggaa
     2881 atgttccaga aacactaaaa aacaaaactt tatactcttt agagatgggt tcattattgg
     2941 ctggggctaa gtatagagga gaatttgaag aaagaattaa agaagttgta gatgaagttg
     3001 ttaaaaatgg aaacataatc ttatttatag atgagatgca cacaataata ggggctgggt
     3061 ctactggaga aggctctata gatgcttcaa atatattaaa gccagcctta gcaagaggag
     3121 aaatacaagt tataggtgca actacaattg atgaatatag aaagcatgtt gaaaaagatt
     3181 ctgctctaga aagaagattc caaccagtta tggtagatga accaactaaa gaggactcaa
     3241 taaaaatatt agaaggatta agagataaat atgaagctca tcataaagtt aaaataactg
     3301 atgatgctat aaaggcagct gtagaattat caactagata tatatcagac agatatctgc
     3361 cagataaagc tatagacttg attgatgaag ctgcatcaaa agtaagatta aaagaaaata
     3421 cacctccttc agaaataaaa aaattagaac tagaaataga aaatatagat aaagaaaaag
     3481 aagaagcagt aagatgtcaa gactttgaga aagcagccaa aataagagat gaacaaggtc
     3541 tacttaaaaa acaattagaa gatgttagag aaagatggaa caagtcatct aaacattcag
     3601 atttagttga tggagaagtt attgctgaag tagtgggctt atggacagga ataccagtta
     3661 ataaaattct tgaagaagag gctgatagac ttttgagact tgaggaaata ctgcacaata
     3721 gagttatagg tcaagaacaa gcagtaaaat ctatttcaaa ggcaatcaga aggtcaagag
     3781 caggtcttaa agaccctaat agaccaattg gttcattttt atttttaggt ccaacaggag
     3841 taggtaaaac agaattatct aaggcattag cagaagtgca atttggagat gaaaatcaaa
     3901 taattagaat agatatgtct gaatatatgg aaaaacatgc tgtgtcaaga atgataggtt
     3961 cacctccagg ttatgtaggt catgatgaag gtggtcaatt aactgaaaaa gtaagaagaa
     4021 atccatattc agttatatta tttgatgaga tagaaaaagc tcatccagat gtatttaata
     4081 ttctgttaca aattctagat gatggtagac taactgattc aaaaggaaga actgtagatt
     4141 ttaagaatac tattgtaata atgacatcaa atgttggagc atctacaatt ggtagacaaa
     4201 aaactttagg atttagtata gctaaaggag acgaagaaga aaaatctcaa tatgaaaaaa
     4261 tgaaagaaaa tataatgggt gaattaaaac aaagattcag accagagttt ttaaacagaa
     4321 tagatgatat aattgttttc cactcactaa atgaaaatca tatatcaaaa atagtacttt
     4381 taatggcagc gaaactacaa gagagattaa aagaaatgga tataaaatta gaaatgagtg
     4441 atgaggctgt taaattaatt tctaaatctg gatttgactt agaatatgga gcaagacctc
     4501 ttaaaagagc tttacaaaaa gagttggaag atgagctatc tgaagcaatc ttaaaaggta
     4561 atgtgaaaaa gggaagtaat gtagtagcaa aagtcaaaga tgaaaaaata gtttttgaaa
     4621 ctaaataatt taatagagtt gcattttaaa atattgcaaa tataagtttt ataatctaaa
     4681 caaaatttta agggcaatga atctataata attcattgtc cttaattatt ttatttaatc
     4741 tttacatatt gtctttcaga tatgctaaaa ttatagtata gaataaaaaa ttaaggatga
     4801 taaaagtatg gcaaaaataa aaacaaaata tgtatgtcaa tcttgcggat atgaaacagc
     4861 taaatggctt ggtaaatgtc cagaatgtac aaaatggaat acatttgtag aagaaataga
     4921 ccaaaaaagc accaaaaaag aggtctttat aatagataaa tcttcatcta aaccagtcag
     4981 tataaactca atagaaagta aagaagaaga gcggtttaca acagatataa atgagttaga
     5041 tagagtgctt ggaggaggta tagttaaagg ctctttagtg cttgttggtg gtgaccctgg
     5101 tataggtaaa tcaacacttt taatccaagt atctagtaat gttgccaatt taggaaaaac
     5161 tgttttatat ataactggag aagaatcaga atctcaaata aaaatgagag ctaaaagatt
     5221 aggtattaac tcagaaaatt tatatatatt tgctgaaaat aatttaagta taattgaatc
     5281 ttatttagaa agtgtaaatc cagaattaat aattctagac tcaattcaaa ctgtcttcag
     5341 tccagaaata tcctcggcac caggaactgt cagccaaatt aaagaaggta cttcaaaatt
     5401 catgaaaatt tctaaaaaaa tgggaatttc aacgttcata gttgggcatg ttacaaaaga
     5461 aggttcacta gcaggaccaa aattattaga gcatatggtt gatactgttc tatattttga
     5521 aggagaaaga tataatactt atagattagt aagagctgtt aaaaatcgat ttggttcaac
     5581 taatgaactt ggagtatttg aaatgaggga tttaggatta gtagaacttg ataatccatc
     5641 taaaatttta atctctgaaa aaccaaaaga tgtagcaggt tcagtaataa tatctactgt
     5701 agaaggtacg agaccaatgt tacttgaatt gcaagcatta gtttctccta caagttttgg
     5761 tataccaaaa agaacatcta caggagtcga ttacaataga gtaggtatgt tattagcagt
     5821 attagaaaaa cgtgttggtt tgcaaataca aaatcaagat gtctatataa acatagttgg
     5881 aggaataaag ataaatgaac cctcaattga tttagggata gccatttctg tagcatctag
     5941 ttttagaaat ataccgatag atgaagatat agctgttaca ggagaagtgg gacttacagg
     6001 agaagttaga gcagtaagct ttatagaaaa gagaatagct gagtgtaaaa aacttggatt
     6061 taaaaagata gtagttccta gaagtaatta tgatgttgta aaagagacca aaggcataga
     6121 aatatggcca gtagataact taagacaagc tataaatata gtacttggga ggaatcaata
     6181 aatatggaga attttctaga taataaaaat atgctatatg cattaaaaat gatatctcct
     6241 ggaactccac ttagattagg tctaaacaat gtactaagag ctaagactgg tggattaatt
     6301 gtaattgcaa caaacgaaga tgtaatgaaa atagtagatg gaggatttgc tataaatgca
     6361 gaatattcac catcatatct atatgaatta gctaaaatgg atggagctat agttttaagt
     6421 ggtgatgtaa agaaaatatt atttgctaat gcacaactta tacctgacta ttttatagaa
     6481 acatcagaga caggaacaag acatagaaca gcagaaagag tagcaaaaca aactggtgct
     6541 atagtcatag gaatttcaca aagaagaaat gttataacag tttatagagg aaatgagaag
     6601 tatgtagtcg aagatatatc taagatattt actaaggcaa atcaggctat acaaactctg
     6661 gaaaaatata agacagtatt ggaccaagct gtaacaaatt taaatgcctt agagtttaat
     6721 gatttggtaa ctatttatga tgttgcatta gtcatgcaaa agatggaaat ggtaatgaga
     6781 gttacaagta taattgaaaa atatgtgata gaattgggtg atgaaggaac tttagtaagt
     6841 atgcaattag aagaattaat gggtacaacc agaatagacc agaaattaat attcaaagat
     6901 tataataaag aaaacacaga aataaaagaa cttatgaaaa aggtcaaaaa tttaaattca
     6961 gaagaactaa tagaattggt caatatggca aaactattag ggtatagtgg tttttcagaa
     7021 agtatggata tgcctataaa aacaagaggt tataggattc ttagcaaaat acatagacta
     7081 ccaacagcaa taatagaaaa cttagtaaat tattttgaaa actttcaaca aattttagat
     7141 gcatctattg aagaattaga tgaggttgaa ggaataggtg aaataagagc aacatatata
     7201 aaaaatggac tcataaaaat gaaacaatta gtcttattag atagacacat atgatgaatt
     7261 ttaaatgaat tttactactt ttttcttgaa tataatatac actttggtct ataatagata
     7321 agtataacta gggggtgaag cattgataag gaaagtaaca agaatattgt ttattttact
     7381 tggatttaca ataggtataa caacctattt aacattaatg aaagattttg aaatattaac
     7441 atttggaaaa gaaacatatg gttatatcgc agctgtagta gcaggtataa taatagccat
     7501 tttaggatat cttatagaac catgggttgt aaataaagta aaagaaattg ctaaaatagt
     7561 ggataaagaa ttgtcaaaat atcctcagac tgatatattg ttagggtcta tgggtcttat
     7621 agtaggattt gttattgcgt atttattaag tggattagta aatagaatac caatagtagg
     7681 tggaatacta tccttactat tatatttatt cctaggatat cttggaatga aagttgcgct
     7741 taaaagtaaa aatgatttat ttaatattgg aaaactagga agattagcaa atcctattaa
     7801 ggataaagat aaggataaag aaaataaaaa agaagtaaaa gccattcctc caaaagtatt
     7861 agatacaagt gtaataattg atggaagaat agctgatatt tgtaagactg ggtttataga
     7921 aggaaaactt attattcctg cttttgtttt ggaggaacta agacatattg cagattcttc
     7981 agatgattta aaaagagtaa gaggtagaag aggtctggat atattaaata taattcagaa
     8041 agaattaaat atagaagttg aaataagtga gagagacttt gatgatatag cagaagttga
     8101 tagtaaatta ttaaagcttg ctcaggtttt aaatggaaaa gttgttacaa atgattataa
     8161 cttaaataaa gttgctcaat tccaaggtgt tgaagtatta aatataaatg agttagctaa
     8221 tgctataaaa ccagttgcta taccaggtga agatatggtt gttcaagtgg ttaaagaagg
     8281 aaaagaggct caacaaggtg ttgcatatct agatgatgga actatgatag ttgttgatgg
     8341 aggaagaaaa tatatgaatg agactataaa agtactcgtt acttctgttc ttcaaactcc
     8401 tgcaggtaga atgatatttg caaaacctaa aaattaatat gttcaaagaa acctataatt
     8461 tgggctgttt attttaaata gatggtccaa atttatttat ttataacgat aatgtttaat
     8521 gtattcttaa agatggggat ttaaatcaga aagcagggag aatttatgag aaaaaagatt
     8581 atatctttct tagcaacatt tatcataata ttaacatcag caagtcagta tagttttgca
     8641 gatgatatat caacacgtgg aaaagtaata tttatagata tgaatagaac tagtatgagt
     8701 aatatgttaa gaataaaatc attaagagaa gaactagaca atagagggta tattggcttg
     8761 atgaatataa gaggcgataa aggaagtgat gatagaaggt catatgctag tatgggtgct
     8821 ggtggtagag ctaatgtggc taatgaagaa gatattaatt ttgaaagctc gagtaaggat
     8881 agaaatatag tttttgaatc agctacagga aaaagtgcaa aaggtataaa taatttaact
     8941 ataaataagt ctataaatga aaatttaaac tttggagagt atggttctgt attagggtca
     9001 ttaggacaaa gtttatctga taatggatta aaagcatctg tattgggtaa ttcagatata
     9061 attgaaaatg gtcaacttat aaaaaataga aacttatgtc taactgcaat ggatgaatat
     9121 ggaagaatac ctaatggaaa tgtagatact ataaataaaa aagacttatc tatgccttat
     9181 ggaatatcaa ctgattatga taaattgatt gtagagacta aagaaactta caaaaataat
     9241 gatgtgattt ttgtggagct tggagacaca tatagattag atttatataa accaaattta
     9301 aatgaaaaaa catatgagag tatgaaagat aacatagaaa aaaatataga tatatattta
     9361 aaaaatatat tttctatggt tgaagaaaat gacactatct acatagcaag tgcatttcca
     9421 agtgatttag attataaaaa taaaagaaga ctttctccaa taataaaatt aaatggagaa
     9481 gggaaaggta tattatcatc ttcaactact agaagagaag gtattgtaac aaatttagat
     9541 gttggggttg aaatattaga caactttaat ataaaaaatc aaaatatggt tggaagaaaa
     9601 tatgaactta ttaacagaga tgataataaa gaatttttga tggatgaata tcaaaagata
     9661 gtatctattt caagcataag gtctacaata ctgaatggtt ttgtaagtat agttttcttg
     9721 tcatggatag ttgctatgat tgcaatttta tttagaaagc atatatcaaa aaattataaa
     9781 gaaacaacat tttttatatt aaaggaactt ttaaaaatag gaatagtaat gccactgtca
     9841 tttatgataa caccaataat gaatttcaaa acaccattag caatatcttt aggaataatt
     9901 ataataacac ttactatata tcttataagt aaagttttaa taaagaacga tttaaaaaac
     9961 atgctgtttt ttactggatt aaccatagta attatggtta ttgatgctgg gtttggctca
    10021 tatttaatga aaagcaatgt tatgagttat gattgtataa ttggagctag atattatgga
    10081 gtaggaaatg agtatcaagg tgtggcaatc ggtagtgcaa tatttacatt tgcaatttta
    10141 cttacatata aaaatatacc aaagtggtat ataatagttt tttctttggt tatcttaatt
    10201 acaagtgcat ctccaattat gggagcaaat gttggaagtg ctatttctga atgtgttgca
    10261 tttttactat ttatactatt gatatataat gtaaagatag atttcaaaaa aatcgtatta
    10321 ttaggtatag cagtagtatt tgtattagga gtttttgtag caatagatat gattttggga
    10381 agtaattcac atttaggtat gtttgtaaaa gaaatttact ttaatggacc aggagaaata
    10441 atacaaacat ttagtagaaa gatagagatg aatttaaagc tagctcaaac cagtgcatgg
    10501 gtaaatatac tgttaactgg aataggtgta attttagttc ttatgattaa tcagattaga
    10561 tactttaagc aactgatgga tgagtatcca attgtattta aaggatttat agcatcaata
    10621 gcaggatgtt tagtaactct tttagtgaat gactctggga ttgtgtcatc agctacagcc
    10681 tttatttatg taattgtacc tatgataaca ttaagtataa atcttacagc attaaaagaa
    10741 taagtactgt atgaataata tgtaaatatt aatacattaa tttaacttct ttatttacaa
    10801 aataaagaag aaagtttata aaaaataaat tataataaaa aagagtttat ctcaaaattg
    10861 attattctaa tcaaatttta agataaactt tttttattat aaagataaat aacatataac
    10921 ctcatatttt aggtagattt ttttcttata tgtttaggag tgcatttaaa tttttcctta
    10981 aaaattctgt aaaataattt tgtattagta aatccatgag cttctaatat actgcataag
    11041 gtatcatttg tgttaattaa atctttataa atatgagaaa tacgcacatc atttaaatac
    11101 tctaagtaag tttgtcccat atactgttta aattttctac aaaaatattc tggttgtaag
    11161 tgtgctacct tagcgatttt gttaatagaa ataggcattt tgtaattaat ctttgtatat
    11221 tctaaaactg gttcaaattt tgacaaatca agtactgttt ttttcatatt tttattacct
    11281 acagatattt taaagtttgt atataattca aataaaagtt caaataataa gcttgtaaag
    11341 agcagattag ctgcaggagg agaactaagt ttaatttctc tcatgttttc taatataagt
    11401 ttaactttac ttgaattttt tttatagtta ttgtcattgg cattaacctt aaagtcaaaa
    11461 tagcaatttt taaaatcagg gatatatttg tttagaaaag acaatggtac ttgcaataat
    11521 atagaggtat tttgataaat acatctagtt gagtgaataa ttccagaatt aatgaacata
    11581 cactcattag cttttaaatt ataagtgtaa ccctccatgt taatttgtaa atcaccagaa
    11641 gttatataaa ttatttctaa tgagttatgc caatgatttg ctacataact accactatca
    11701 tcaaatagtt tgaaattaac atctaaatta tcaataggtt caattatttc atgtgaaaaa
    11761 ttatttctgt tcataataat atcctcctta taattttaaa ttataaatag taaatttata
    11821 atgttcaata ttaaaaagat aatatagact aataaattag ataataaaca tacttaagaa
    11881 aacggataaa atcaaatata ataactgtat attaaaataa cttaaaaaaa ttattttatt
    11941 ataagtgcaa aatataccta tttaaataag ataagagggg aggattttat gtgtatataa
    12001 aaggtgtaaa tttaggaggt tggttagttt tagaaaaatg gatgacctca agtttatttg
    12061 aaggcacaga agctgaagat gaatactatt taccaagaca gctttcaaga gaggcatatg
    12121 aatctagaat taagactcat cgtagtgaat acatcacaga gagagatttt gcaacgatta
    12181 aatctatggg atttaattca gttcgtatac cagtaccata ctttattttt ggagattgtg
    12241 aaccattcat aggatgtgtt aaagaacttg ataaagcatt tgcttgggca gataagtatg
    12301 ggcttagtat attgatagac ttacatacag ttccaggaag ccaaaatgga tttgataatg
    12361 gaggaatatc aggtatatgt agctggtcac aaaatccaga atatgtagcg tttacattaa
    12421 atgtattaga aagacttgca aaacgttatg ggatgaggca tgaattgtat ggaatccaaa
    12481 ttttaaatga gcctataaca gaaagaatgt ggaacataat gaatgttcca aatcggttta
    12541 aggctgtaga taaggagatg gcaaggggga gtaaacctaa ttcattagag tttttacgtg
    12601 atttttatat aaaagcatat agggtaatgc gtccttatat gagagaggaa aacgttatag
    12661 tgtttcatga tgcatttgaa ttaaaagcat ggaaagactt tatgagagag gaagaattta
    12721 aaaatgttgt tctagataca caccaatatt tgatgttagc tgaagcagat ggatgtgaac
    12781 aaagtattga tagctatctc aaatatatta gagaaaatta tgctaaagat attttacaaa
    12841 tgcaaaaata ttttcctgtt atatgtgggg agtggagctt atttaattca tatgtttgtg
    12901 gtatagatac aaatggaggt caatcacctc taaatgggat tgaatcaaat atagataaat
    12961 tatcaaaaga cgataaaaga gaattatata ggaagatagc aaaagcacag cttgatgcat
    13021 ggagaaatgg aagtggtcac tactattgga attataagtt attactagat acagttaatg
    13081 aagaaggttg gataggatgg gatagttggg acctaggcaa atgtgtagct caagaatggt
    13141 atcctataga atattaaact aaaattggag gaaaagatat gattaaaaac cctatacttc
    13201 caggttttaa tccagatcct tgtatttgta gaaagggaga tgattattat cttgtagttt
    13261 catcttttga atggtttcca ggtatacctg tttatcactc taaagactta aaaaattggg
    13321 agttatatac tcatattttg acagatgaaa caaaaataga tttgaagaag cttccttcat
    13381 ctaaaggaat ttgggcaccc tgtttgactt attgtgaaga agaagattta ttttacatag
    13441 tttatggaat tatgaactct atgaatgcaa gatattttga tgttgataat tatttaatta
    13501 catcaaaaga tataaaggga gagtggagtg aaccagtata tctacactca tcaggttttg
    13561 atgcgtctat ttttcatgat gatgatggta aaaagtggat tgcatcatta gactgggaga
    13621 ctagagaagg atatgaaaaa cctggtgtta tatgtttagt ggaatactgt acaaaaaaga
    13681 aagaaatagt aggatatcca aagagaatat ggtcaggggg aactgataga ggttgtattg
    13741 aagcaccaca tataacaaag cgtggagatt actattatat aatgtgtgca gaaggtggaa
    13801 ctggatatgg tcatagtgta actatgggaa gggctaaaaa tatatgggga ccttatgaaa
    13861 aagattcaat gaatccaatt gtaacttcaa ttccaggcga tttttatgaa agacatgacc
    13921 ctgaccatct aaagcctaaa tactataatc cagaatcaaa acttcaaaaa tctggtcatg
    13981 gaagctatat agaaacaaca tcaggtgaag tatatttagt tcatctaaca tctagaccat
    14041 ttgtaccaga gttaaggtgt actttaggta gagaaacagc tattcaaaaa atgaaatgga
    14101 caaaggataa ttggcttcga atggaagatg aaagtaattt ggcaaaagaa tacgtatcag
    14161 aaagtaaact agaagaacat ttagtttcat ctataccaag ttttgatgac tttgattcaa
    14221 atgaattagg tttacagtac tatgctccta ggatttcacc attatcattt gcagatgtta
    14281 agtctagacc aggatatgta aggattagag gacaagaatc aagaacatca ttaaataaag
    14341 taagcatatt ggcaagaaaa ttaacttcag tatatgcaag aataaccact aagatggagt
    14401 tttacccaga ggtacatcag catagtgcgg gtttgataat gtattatgat aatatgaatt
    14461 atataaattt aagaaagtat tatagtgaaa cattgggtca aagtgcacta tcaataatac
    14521 atcttgaaaa tggagagaaa acggaatttt taaatactag gataccaata aaagatatac
    14581 caatatatct tcgtctgtat atacaaggtc gtaagtcata ttttgaatgg agttatgatg
    14641 aaaaaaacta ccagagaata ggaaaggtat ttgatacaac taagttttca gatgagtact
    14701 gtaaatatgg agaatttaca ggtacgttta taggattaac ttgtgcagat cgtgtaaagc
    14761 ataaacacta tgctgatttt gatttttttg aatatatagt agatgaatca aaagatgtcg
    14821 attaataaaa tcattactaa aattttcaga gcaaaggtaa atcttatgct ctgaaataag
    14881 aaacattaga aggcaaatct tacaaaaagg agtatggtat tatgaataca gaagttaatg
    14941 ttaaagcaac tacgattaga tatgaaaaga tttcattttt tgaaagaatt tcttatggtt
    15001 gtggggattt ggggtgcaat atcatatact cagctatgtc agcatttttg ctgttttatt
    15061 atactaatta tgcagatgtt agtgcagcag ctgtaggtag tattatgctt gtatctagga
    15121 tactggatgg gtttagtgac ttaacaatgg gaattatagt ggatagaact aaatccaaat
    15181 acggtaaggc taggccttgg attttgagaa tggcgattcc ttttgctata gcagcagtat
    15241 tattattttc tgttccatca aatcttggta tcacttctaa attaatttat atttttatta
    15301 catataactt agtttctact gttatttata cagcaatcaa tgtaccctat gcaactctaa
    15361 attcacttat aactcaaaac cagtatgaaa gaggagtatt aagtatattt agaatgatat
    15421 tagccacttg tgatacctta ctaataaatg gacttacact acctctagtt gaatactttg
    15481 gaaataattt atcagcatgg actaaaacat tctttgtatt tggaatagca tctataatgg
    15541 tattttttat aacatttact ggaaccaagg aaagagtaaa agcagttaag caagatagga
    15601 atgaagtaat accatttaaa ataggtatta aatctttatt tagaaataag tactggatac
    15661 aaataacatt atgtttagtt tgcatattta ttgtatttgc gataaatggt ggttcatccg
    15721 tatattatgc aaagtttatt ttgggagatg aaaaattatt tggtcctata aatatggttt
    15781 caaatatatc ccaaattata gctatgttta tggttgctcc ttttataaaa aaatttggaa
    15841 aaagaaatgt attaatagta ggttcaataa tattaatatc gtcaaatgtt atgtttataa
    15901 tagttggaca aaatcatata ggagtaatat ttgcaagtgc tataaaggga ataggaagtg
    15961 ctggaatagc atcaacaatg tttgctattg tttcagatac tattgaatat ggagaatgga
    16021 agactggata tagaactgag gggcttataa atagtgcatc tagttttggg tttaaagtgg
    16081 gaaatggatt aggttcagca atattaggtg cagtattatc aattggtggt tatgtaggaa
    16141 catcagctac tcaaagtgat ttagctatag tatcaataaa agtatgtttt atatacctac
    16201 ctatatttat aacaatatta caaattatca taatgtcttt ttataagctt gataaagaat
    16261 atagtactat acttaatgaa ttaaatacta aataatgaaa aagtttctta taaagtctta
    16321 agtttaatta aggtctattg taaatgttcc ctataaaaac tattatatat ttatgattat
    16381 tgaagaattt ttagagaagc gaataataaa aatataagaa gactttaaat gatgaaatgg
    16441 ttggtttttt agaaatgatt taataagtta ttgttattta aagcattata agtggacaat
    16501 taatttttaa tttatggaga tgatatttaa caattagaag taatatataa tatgcactaa
    16561 gaagcaattt ttcaactatt tatgaaggtg gtggtacaag aaaagtatca cctttttttt
    16621 gttatacaaa aaaacagttg actttttata tgaacccctt ctataatgtt actgaaatta
    16681 agtaaaatga ttgaacaaat gtaaagataa ttgaaaagct tgattttgga ggaaagcaaa
    16741 tgaaaaaaat tggagactta agattaatga tgaagtgttg ttcactttat tacgaagata
    16801 atttgaatca acaggaaata gcaaatcaat tgggaatatc aagaccaaca atatcaagaa
    16861 tattaaaaga agcttttgaa caaggaatag taaaaataca gatagttgat gttttgaaaa
    16921 atgattacca aaaaatagag agaagtttgg agagaaaata taaattaaaa gaagtaatag
    16981 tagtagatga taaacaggat gcactaacac aaaaacaaga actagcaaga gctgtatctg
    17041 aatatttaac tagagtagta aaggaaaatg atataatagg tgtttcaata ggaacaacac
    17101 taaaagaaat accaagatat gtggaaaaaa gtaactgtaa aaatgttaca tttatacctc
    17161 tattaggagg gattggagac aatgaaatag atattcatgc aaatcaaata gccgtaagtt
    17221 tagcgagggc ttttggaggt gattttaaat tattacatgc tccagctgtt atgtctgact
    17281 tgtctacaaa agagaaactt tgcaaagacg aaaaaattaa agaagtacta gatttaatag
    17341 ataaaacaac tatagcaatt gtgggtatag gaaatcctat gagtttaaac tcaacaataa
    17401 tggcttctgg atacatgaat gaagcagata tagaagattt aaaaaaatac aactcaatag
    17461 gtgcaatttg tttacaagcc tttgataaag aaggaaaaac ttctatactt gaatttaatc
    17521 aaagagtatt aggagtgaaa ctggaagatt taaaaaagat aaaaagaaca ataggtgtcg
    17581 cttcaggaga tgaaaaaata gaggcaataa aggcatcatt aaaagcgaaa tttataaata
    17641 gtttagcaat taatcacagt ttagcactta aattattaga agattgtgac taatagaaag
    17701 atagataaaa gttaacagga gggaatgtat atgtcaatat caaaagaaac attgcttgaa
    17761 atgtataaga gaatgaatca ggctagaaaa tttgaagaga aagttagttg gttttttgca
    17821 agaggtatgg ttcatggaac aactcactta tctgtaggtc aagaagcgtc aagtgtggcg
    17881 gctgtaatgg ctttagaaaa aggagattta gtatcattaa ctcatagagg acatagtcaa
    17941 tttataggaa tgggaataga cctaaataaa atgatggctg aacttatggg taaagaaact
    18001 ggattttgta agggaaaagg aggctcaatg catatagcag atattgagtc aggtaactta
    18061 ggagcaaatg gagttgtagg tggaggtctt acaatagctc caggagctgc acttacacaa
    18121 cagtataaaa aaacaggcaa aatagtttta tgtagttttg gtgatggagc atcaaatgaa
    18181 ggtacttttc atgaaggaat aaatttatct tcaatatgga aattgccaat aatattttac
    18241 tgtgagaata atttatacgg tatgtcaaca agcataaaac gtcatatgaa tatagaaagt
    18301 attgccacta gagcagcttc ttatggaata gaaggaatat caatagatgg atataaccca
    18361 atagaagtat acgaaacagt acaaaaagct gctgagaagt gtagaagagg agaaggacct
    18421 gtattaattg aaagtagaac ttatagatgg ttaggtcatt caaaatcaga tgcaaatgta
    18481 tatagaacaa aagaagaaat agaatcatgg aaagcaaaag accctattga atttttaaaa
    18541 aattatctta tagaaaataa tctatctaat gaagatgaat tagataagat tcaagaattt
    18601 gcaaagcaat caatagaaga tgcagtagag tttgctcaaa atagcccaaa tccaaaaata
    18661 gagtctttat tagaggatgt atatgcagac taagaatggt aaaggaggaa aataaaaatg
    18721 agtacaaggg aattaacata tgcacaagca ataaaggaag caatgtcaga agaaatgaga
    18781 agagatgaaa atgtaatatt tatgggagaa gatataggaa tatatggagg agcatttggt
    18841 gtatcagttg gtatgataga tgaatttgga ccagaaagag taagggatac tcctatatca
    18901 gaagcagcta tagcaggagc ggcagcaggt gcagcagcaa ctggcttaag acctattatg
    18961 gaagtaatgt ttatggactt tgtaacaatt tctatggatg caattgtaaa tcaagcggca
    19021 aaaatgagat atatgtttgg aggaaaagca caagttccaa tggttgttag atgcccagga
    19081 ggttctggaa ctggttcagc agagcaacat tcacaaagtt tagaagcttg gttttgtcat
    19141 gtaccaggag taaaagtagt tgcaccttct acaccagcag atgctaaggg attgttgaaa
    19201 gcagctataa gagataataa tccagtcata tttgttgaga ataaattgtt atacagaaaa
    19261 aaaggttttg tgccagaaga tgattatgta attgaaattg gaaaggcaga tattaaaaga
    19321 gagggtactg atgttacagt aattacttat ggaagaatgt tacaaagtgt tgaagaagca
    19381 gcagagactc tatcaaaaga aaatataaat gttgaaatta tagatttaag aactttatat
    19441 ccattagata aagagactat agtaaagagt gtatgtaaaa ctgggagagt tttaatttgc
    19501 catgaagcag ctaagactgg tgggctaggt ggagaaatat cagccctgat aacagaaagt
    19561 gaatcatttg attatttaga tgctccagta aaaagaattt gtggaaaaga tgtgccaata
    19621 ccatataatc cagagttaga aaaagcagtt gtcccaagag ttgatgaaat agaagaagca
    19681 ataaaatctt taattgttag ataaggaggg atagatatgg tggcaaataa aataaaagcc
    19741 acaccagcag ctagaagtca ggcaagaaaa gacaatataa aattagatag gctgattgga
    19801 agtggagaaa atggaaggat tcatttagta gatgtgttaa attatttaaa agataataaa
    19861 gcaaatacta ctcctttagc tagaagaata gcagaagatt taaatataga tttagaaact
    19921 attgtaggta ctggatataa tggaaagata agaaagtgtg atgtggaaaa attaactgga
    19981 aaagaaacta ttgtatcaac taatacatct aaatctagtg agaaaaaaga gctgaaaatt
    20041 gaaaatgaaa attcaagaat gtttaacaca gtagaaggaa tatttgaaaa gccaaatcca
    20101 atgagagcta ctgtagcaaa acgaatgtca gaaagttatt ttagtgctcc tgtatttaca
    20161 ttcaatatag aagtagatgc tacagaattg aaggtactta gagcaaagtt aatcgacaca
    20221 gtaaaagaat caactggagt aaaattaact atgacagatt taatagtaat ggcagtatct
    20281 aaaatacttc caaatcatca agccttaaat tcagcatgga cagatgaggg tatatttaga
    20341 tataaagatg taaatatagc tattgcagtt ggtttagatg aaggcttata tgttccggta
    20401 gtaaaaaatg caaacaaaaa gtctttaaaa gaaatagcaa aagaaagcaa agaacttgcg
    20461 gaaaaagtaa agacagggaa acttatgcca gcagaccaag aaggtaatac atttacaata
    20521 agtaatgttg gaatgtatgg tataactaca tttacaccta taataaatat gccttcaagt
    20581 gcaatcttag gggtaggtgc tacacaagat aagtttgttc cagtaaatgg agaggctaaa
    20641 ataaaaccta taatgaactt atcattaaca tcagaccata gagtaataga tggaacagta
    20701 gctgcaaaat tcttaaaaga tttaaaagaa ttgttagaaa atcctttatc aatgcttgta
    20761 taggttttga tattttgtta ctgatataaa tagtttgaat agattatatt attataattg
    20821 tttcagtaaa tttaaagctt ttatatatta agaattattt ttaacagtaa ctatatgtga
    20881 atatgatttg cttaattaaa atatatgaaa ataaatagga ggagagccta tgtcagtaga
    20941 agtaataatg ccaaaagcag gagtagccat ggaagaaggt actatagtat cttggctaaa
    21001 gcaagaaggc gaagaagtaa agattggaga acctatacta gaaataacaa cagataaagt
    21061 taatatggag atagagtcag aaggagaagg tacacttgct gtaatcatac ataaagagga
    21121 aggtgaagta ttaccagtat ttacagtaat aggtgtaata gcagaaaaag gggaaaatca
    21181 agaggaagta aaagctaaat atttatctgg aaatgtttca aaagaagata tagttgaaga
    21241 gaatcaaaat atagaattta aagaagaaaa aataaacaaa aaagagtgta atcatgatta
    21301 tgatgtagtg gtaataggag gaggacctgg gggatattta tcagcattaa aagcagctct
    21361 tttaggtgga agagttgctt tagttgaaga aaatatacta ggaggtactt gcttaaatag
    21421 aggttgtatc cctacaaaga cttatataaa aacagcagaa atattagaag aaattgacca
    21481 attatcaaag agaggtgtaa aagtaactgt agataaagaa caggacatta agaaggccat
    21541 aaaatataaa aatagagtag ttaaaaaact tacagctggt gttggcggat tattaaaaag
    21601 tagagatgta gatgtgttta atcttaaagc tagtgttaaa gaagagcata aagtaatttt
    21661 atcagatgga aaagttttag atacagaaaa tataataatt gctacaggtt ccaaagtaag
    21721 gatattacct ataaaaggaa tagaatcaaa tttaataata actagtacag aagcattaga
    21781 tttggaaaca gtgccagaag aattagtaat aattggtggt ggagtgattg gttgtgaatt
    21841 tgcagagata ttcaattcaa gaggttctaa agtaactata gtagaaatgg aagatagagt
    21901 aatcccaaga atggataaag agttaagcga atctttaaaa tattctctta gtaaaaaagg
    21961 tataaatgta ttaactaaaa agaaagtttc tgaatttaaa gaagaaggaa ataacatatt
    22021 agtgtgcatt gaaggtgaag agccaataaa agcagattta tgtctatatg ctataggaag
    22081 agaagctaat ctttctggca tagaggactt agatattaaa atagataaag gttcaatagt
    22141 tgtaaatagt aaaatggaaa caagtatacc aagcatatat gcagtaggtg atgttactgg
    22201 aggtgtaatg ttagctcatg ctgcttttaa aatgggagag gtagcagctt caaatgctct
    22261 aggtgtgaat aaagaagttg acttaggtgc attacctagt tgtgtttata caattccaga
    22321 agtagcatca gttggtatta cagaagagga tgctagaaaa aaatataatg taaaagttgg
    22381 taagtttaat tttgctggaa atggaagagc tttagcttct ggtcaagaac aaggatatgt
    22441 gaaggtagta gcagatgcta aatatggaga aattttaggt atacatatgt ttggatgtgg
    22501 tgtagcagaa cttataaatc atgcagcatc atttaaagcg ttagagatac ctactgatga
    22561 agcaagtgag ttgatatttg gtcatccatg tacttcagaa gctttaatgg aagctttggc
    22621 agatgtaaat ggagaatgtt tacacttacc taaaaaataa atctaaacat aaattattaa
    22681 tttaagtcta ttattaatat taatttatat cataaaattt taaaacactt acttagccat
    22741 accccttatt atgcaaataa tttatttgca taataagggg ta
//
LOCUS       DACULQ010000048        22372 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.048, whole genome shotgun sequence.
ACCESSION   DACULQ010000048 DACULQ010000000
VERSION     DACULQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.048"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(3..166)
                     /locus_tag="JA154_07535"
                     /pseudo
     CDS             complement(3..166)
                     /locus_tag="JA154_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895106.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(345..485)
                     /locus_tag="JA154_07540"
     CDS             complement(345..485)
                     /locus_tag="JA154_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995718.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(743..1903)
                     /locus_tag="JA154_07545"
     CDS             complement(743..1903)
                     /locus_tag="JA154_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HAT7995719.1"
                     /translation="MKLNKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGSVSAVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSISDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            complement(2074..2277)
                     /locus_tag="JA154_07550"
     CDS             complement(2074..2277)
                     /locus_tag="JA154_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HAT7995720.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            complement(2364..3398)
                     /locus_tag="JA154_07555"
     CDS             complement(2364..3398)
                     /locus_tag="JA154_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HAT7995721.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTATSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKEAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSKFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            3689..3838
                     /locus_tag="JA154_07560"
                     /pseudo
     CDS             3689..3838
                     /locus_tag="JA154_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3920..5275)
                     /locus_tag="JA154_07565"
     CDS             complement(3920..5275)
                     /locus_tag="JA154_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HAT7995722.1"
                     /translation="MKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRAGV
                     KKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMDLF
                     LKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVEEC
                     MLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIIVNIVTSSQ
                     EDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILGLK
                     FKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKVYG
                     IEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKDAI
                     RDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKLIR
                     E"
     gene            complement(5308..6030)
                     /locus_tag="JA154_07570"
     CDS             complement(5308..6030)
                     /locus_tag="JA154_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HAT7995723.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(6030..6359)
                     /locus_tag="JA154_07575"
     CDS             complement(6030..6359)
                     /locus_tag="JA154_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HAT7995724.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            6689..7615
                     /locus_tag="JA154_07580"
     CDS             6689..7615
                     /locus_tag="JA154_07580"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086869.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     glyceraldehyde 3-phosphate from fructose 1,6,
                     bisphosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAT7995725.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(7750..9381)
                     /locus_tag="JA154_07585"
     CDS             complement(7750..9381)
                     /locus_tag="JA154_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT7995726.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(9864..10901)
                     /locus_tag="JA154_07590"
     CDS             complement(9864..10901)
                     /locus_tag="JA154_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAT7995727.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(10920..11705)
                     /locus_tag="JA154_07595"
     CDS             complement(10920..11705)
                     /locus_tag="JA154_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HAT7995728.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(11738..12871)
                     /locus_tag="JA154_07600"
     CDS             complement(11738..12871)
                     /locus_tag="JA154_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT7995729.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(12919..14046)
                     /gene="hadC"
                     /locus_tag="JA154_07605"
     CDS             complement(12919..14046)
                     /gene="hadC"
                     /locus_tag="JA154_07605"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HAT7995730.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(14046..15272)
                     /locus_tag="JA154_07610"
     CDS             complement(14046..15272)
                     /locus_tag="JA154_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HAT7995731.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(15287..16087)
                     /gene="hadI"
                     /locus_tag="JA154_07615"
     CDS             complement(15287..16087)
                     /gene="hadI"
                     /locus_tag="JA154_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HAT7995732.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(16126..17325)
                     /locus_tag="JA154_07620"
     CDS             complement(16126..17325)
                     /locus_tag="JA154_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HAT7995733.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            18002..19000
                     /locus_tag="JA154_07625"
     CDS             18002..19000
                     /locus_tag="JA154_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HAT7995734.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            19362..19889
                     /locus_tag="JA154_07630"
     CDS             19362..19889
                     /locus_tag="JA154_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HAT7995735.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            19916..20512
                     /locus_tag="JA154_07635"
     CDS             19916..20512
                     /locus_tag="JA154_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT7995736.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(20577..22160)
                     /locus_tag="JA154_07640"
     CDS             complement(20577..22160)
                     /locus_tag="JA154_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HAT7995737.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASGPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(22225..22362)
                     /locus_tag="JA154_07645"
     CDS             complement(22225..22362)
                     /locus_tag="JA154_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995738.1"
                     /translation="MKNKKFNVITLIILDNHIYAINKEQISTNSKRIYFEKSRFFSDY
                     K"
BASE COUNT         6929 a         4063 c         2672 g         8708 t
ORIGIN      
        1 tcttaccagt tttttatcca cttttctaat gaagttattt tttcatatgt taattcctct
       61 tgaaataatg atactaaaag actttttata aaattataac ttaaaagttt atgtattttt
      121 ttgatatgaa tttatagtac ttatcttctt taattgagat tgtgtatatt taactaaaca
      181 ttacaacaat aattctatga aatcaaatca attatttaca tattttttta gaacaataat
      241 aagtatatat tcacaaaatt ttgtgttaat atttttaatt tgtgatataa taaaagcaag
      301 gaaacatatt ttacttaact ttgagtgatg tttccttcaa caaattattt tgaagattta
      361 cttttctgga actttatatt aagttcccag ccactcttag ttggtcgctc agagtggctc
      421 tttaatttat cgtaaatgta actggctata acaccagtta taatactcat cattagattt
      481 tccatagttt tcacctcctc tcataagaaa gtgaagaaac ttccatggaa acaccactca
      541 tagctttttc aaatatttat tccttgccat attaattata tcataaatat tggaaacata
      601 aaattttaat acttatcgtc tttattatta ttttttataa ttaataattt tttttaataa
      661 tacataatat aaatttatat ttcttcaata aaaaagacaa tcttaattaa ttgtcttttt
      721 taatatatat ttggctaata cattatttta cacaaacatt acaagggtct attttacctt
      781 gtgacttagc ttgttttaat gttactttat ttattgtttt acttcttttt agtgtagaac
      841 aattttttgt tagatgatat gattttccat taggagttac atatactgta cttttatcag
      901 ttaatccagg attattagag ctaggctcat tacttggttt gttcgtatta tttgaattag
      961 tatcttttac aatgcctgta tttgaatccc caacattatt agttatattt aaacattgtt
     1021 ttataacaac atcatcaata tttccaatag cagttatttt acttgcattt tttaatatac
     1081 tcttattact tccatttcca actaacacta taggtgtttt ttgtgcaatt gtagaaccaa
     1141 ctaaagaata aactaaatca gtcccacttg ctatgtaaaa ttcttttgag ccattataaa
     1201 atttatttat tatttgttta tttgtatcgt atctatctac tccacctatt cttgttgctt
     1261 tagtttcatt tattaactta tcactaatac tgacttttcc accaattgca taagtttcta
     1321 atccatctgt agcaaaactt gtagtgtttc catcagttaa tactattgga gatttatctc
     1381 taactgctat agaagcaaca ctcatagcat ctggttcacc tttaaaagca ttagttaata
     1441 ttactttttt aacagcactt acacttccta tttcttttgc aactttatga cttgtatcta
     1501 ttctatcagt tccacttatt ctatttatgc ttattttttt atcacttaat attttctgaa
     1561 ctgatgctcc tatcgaattt tttcctccga ttagatatac ttcttttacc ttttctaatc
     1621 tttttaatgt ttctgaaggg attgtatctt tcttagttaa caatattggg gcatttttag
     1681 ctcctgacaa tccacttgct gacaatccat cagataatcc attttcagaa ttaataagta
     1741 ttgcaatatc ataattttgt ttatctgcta ttaaacctgc tgtttcatac ttatttgtac
     1801 cttttattga attaatagaa ctaagagcgt ttgatgttgg acaactagca atcaataatg
     1861 aaacagacat ccctattgcc aatagtgttt tgtttaattt cataatgaaa tcctcctata
     1921 aattttataa tattacttaa atattataat tctacataaa agaatatttt cctttatgaa
     1981 attagtttat atttcaaaat tttgtcactt tttaaatcta tatactaaaa aatagactat
     2041 ttgtaacagt ctatttctta gtatatcaaa tatttattta ggtttacgtt tctttgtact
     2101 agtttttgta tatgatattc cagttccagg tatactcaat gtagctgatt ttcttccttt
     2161 tgaattaact gacactctag cacctttaac accagcactt atacctacac tcttcttacc
     2221 taaatttaat ttgagtcctc ctcctagatt tatgcttttc ctaaagttaa atcccataga
     2281 tataaccttc ccttctatat tttaaatatt tatacttaaa attatatttg ttatacactt
     2341 gccatcattg gtatatctag catctatatc tttttacgtt ttttcttctt atatactact
     2401 ccagcaacta cagctgctgt tccagctcca accccataag tagcatttct acttttctct
     2461 tcttctcttt tttctaaacc ttcatcatat acacctttta acttagtttc aatagacttc
     2521 atttcatctg gtatactaaa atcttcttca ttgtaacctt gattatatac actttctttt
     2581 gctttaggta acttaacctt accattatca tatccttctt taaaccaatt agcatattta
     2641 acattaccta tagttgatgt gtcaaatctt tctcctttga tagcactctc aaatccttga
     2701 gtagtgtaat cattttttaa ttgttcttgt atttcgtcat aagcattatt ataagcatct
     2761 ataaatttag aatctatgtt ttcttcaaat gtcatttttt ctttatcttt attgctatcc
     2821 tctatccctt tagctttata ttcttctatc tttttagtct tatattcatt gtatccagtt
     2881 gaatatccgg tatcataagc attttttaaa acttcttttt catatgtatt attagattta
     2941 gcacctgtaa gccctagatt atatccagtc tctttagcct ccttttcttc ctcttctaat
     3001 ttagccttac ctttttcata tccttctgag tactttgatt catatgtatc actataatct
     3061 ccactataat ttgatgaact ataatttcct ttatacccat cttcataccc ttgattatat
     3121 cctttttctc caacagacct cttttgcttt tcttcattat taaccgaagt tgctgttcca
     3181 gaactgcttc ctcctccagt ataaggacaa ccaccattat tatgtaaatg agctggataa
     3241 ccaccacaat gatagtgata acttcctaat ccagatttat ttttattatc acgatgacct
     3301 cctgatgaat cagttcttcc tgaatgagca tacgaaaagc tccctacact aatcatagtt
     3361 atactcattg caacagctat taactttttc ttaaacataa tctatttccc cctaaaatat
     3421 tctatgattt tattatatca gtcaatatat tatgtttaaa ccgttaaaat tcgacaatgt
     3481 aaatatttga tgaattgtat ttgtaaataa atcttttagc atatcttgaa tatctgtagt
     3541 tgttttgaca tcatattctt caataagttt tttaatattt cgtttttgtt aaacataaaa
     3601 agcgtgcact tttctttgta tagtgatatt tgatatttta attatcacta ctttagaaga
     3661 atacacactt tattttaaaa accctgatac tatgttatta gaaaatggta ctaatataaa
     3721 agatatccag catatactcg gacatgctaa aacttcaaca actatggata catatttaca
     3781 tgtaactaac aacatgagaa accagacggt aaatattttt gaaatcattg tagaataaat
     3841 ttgccaccca aaaataatta tggtggcaaa taggtggcaa atctactttt aagacacact
     3901 ataaaagtct gaaaccctgt tattctctaa taagtttaac tacagtctca caatgtggcg
     3961 tatttgggaa catatccata cacttaacca tcttaatctc ataaccatac cccttaaaat
     4021 caaccaaatc aacaaccaaa gacttaggat tacaagaaat ataaacaatc tccttagcac
     4081 caaatccaca aatatcccta atagcatcct tatgaatgcc tggtctagga gggtcaacaa
     4141 taatcaaatc aggcttgtcc tttaaatcct taacaacctt agccacatcc ccagcaataa
     4201 actcacaatt agtaagtcca ttcaacttag cattctcatt agcagcaaca acagcttcct
     4261 caactatctc aataccatac actttcttag cagcaccagc cataacctgt ccaatagacc
     4321 cagtaccact atacaagtca aatacaactt tatcattgta atctccaata aaatccctag
     4381 caatactata aagaacctca gctcccttag tattggtttg gaaaaacgaa aatggtgaaa
     4441 tcttaaactt aagacctaaa atttcttctt gtatataatc tcttccatac aatactctta
     4501 actcatcaca ttggactgca tctgctaaac catcattaat agtatgaagt acacctacta
     4561 actcagcttt taaatcaatc cctaaaagca tatctttaaa ctcatacata tcaaaatctt
     4621 cttgagaaga agttactata ttaactataa tctcattagt gtgaatccct tttctaacta
     4681 caagatgtct caaatatcct ttgtgattca ttgttctata ataaggaagc tttttttcat
     4741 tgaaaaactc tacactagaa actaatatct ttgagaagtc ttcatcaacc aacatacact
     4801 cctcaacagt ttgtatatct atatgctttc cttttttatg aagacctaat gtcaaaggtc
     4861 cattttttac ttcatctcca aaagtatatt ccatcttatt tctataatat ttgttttctg
     4921 gacttccttc tatccctaaa aactgaaatc caaataaatc ttgctttaaa aataaatcca
     4981 taacttgttt ttcttttatt tctaattgtt tttcatatgg cacagacaat agtgtacatc
     5041 ctccacactc tctaaagtgc ttgcaagttt cttcagtttc aagtggtgag ttctctaata
     5101 attctatcat cttaacttca gctttcttac ttctaacttt tttaactcca gctctcacct
     5161 tttgaccact tattccaccc ttcatatgga taactctatc tccaacttgg cttaaagatg
     5221 taccaccaaa ttccatttta tctacttcaa attctataac atcttttttc ttcacagaca
     5281 ttcctccaat ttactatagt atctttatta tctttcttca atcaatttta cttcttcatc
     5341 tgttaactct ctatactcac ctaactctaa actttcatcc aattttaaat tgcccataga
     5401 caatcttttt aagtatacta cttttttgcc tacgctttca aacattcttt ttacttggtg
     5461 aaacttacct tcatgtattg taagctctat ttctgattca tcatcacttt ttaatatatt
     5521 taactgtgat ggcatagttt tatatccgtc atcaagcact acaccttcta aaaaagcttc
     5581 tacatcttct tcagtaacta ctccatcaat ttttgcataa taagtcttag gcacatgctt
     5641 cttaggagat aaaactctat gagaaagctt tccatcatta gttaaaacta ataatccctc
     5701 tgtgtcttta tctaaccttc caacaggaaa tggttcaaaa gctaagtatg ataaatcaat
     5761 taaatctaat actgttgggt catatttatc tgtagttgct gatatatatc catcaggttt
     5821 attcatcatc aaatatatat attctctata tataacttct tctccattaa ataatatctc
     5881 atcattttca gtgtctactt gagttcctgg atttgaaact tcacttccat ttactactac
     5941 agacccttgt ttacaatact tctttatttc acttctgctt ccataaccta aatttgaaag
     6001 aattttatct atacgttgct ttttagccat taaaaatgct ctccattctc ataatatttt
     6061 tctttttcaa atactgtaaa tgctaaatcc atactttcta catctaaatt atttcttata
     6121 ctatcagtta ttattcttgc tactatatct tgtacttctt gtcctctatc aaaccaagct
     6181 acttcaacaa aaggatatac atctgctatt ttaccatctc ttattgcaac tgactttata
     6241 cattctattt caaagtagtc tcttggacat tttacagctt caactaaatc atttatcatt
     6301 ttttcactta ttttacatat atcattttca ttaattcctc ttatttttat ctgtggcata
     6361 gtttatctcc tttattttaa atttttagtg aattatcatc cacttaattc ccttcgataa
     6421 cgtttgaagt ggtaacttct tggtcaagac aactattgtt atcagtatct ccaaactaat
     6481 aaggtagttc ctacccttat agttagcatt atatagctaa ttttcctaaa ttgtttatat
     6541 tctttattat tttatttaat cctattgtct tctatttaac attatacttg taatttatag
     6601 ttattacaat ttactatata ctttttttga tttaacgtta taatataagt gatgggtaaa
     6661 ttttaaaagt taataggagg agtttaaaat ggcattaatt actacaaaag aaatgtttaa
     6721 aaaagcttac gaaggtggat ttgctatagg tgcatttaac ataagtgact tagaacaatt
     6781 acaaggtgtt ttaaaagctg ctaaagctaa aaattcttat gttatgatac aagcttctat
     6841 gtcagctgtt aaatatgctg gacctcatac attagttgaa atggttaagg ctgcttctga
     6901 tgaaataggt gtagatgtag ctcttcactt agaccatggt ccaaatatgg atgctattaa
     6961 aacttgtatt gatgctggtt tctcttcagt tatgatagat ggttctcatt ttgactttga
     7021 agaaaatgtt agaataacta aagaagctgt tgaatatgca cactctaaag gtgttgttgt
     7081 tgaagcagaa cttggtgttc tagctggaac agaagatgat gttacttctg atgttcacaa
     7141 gtatactcaa ccagctgagg cagttgagtt cgttgaaaga acaggtgttg attcattagc
     7201 aatagctatt ggaacttctc atggtgcttt caaattcaaa ggagaagcta aattaagatt
     7261 cgatatacta gaagaaatcc aaagcaaatt accaggtttc ccaatagttc ttcatggtgc
     7321 atctgctgtt gaccaaaacg ctgttgctac atgtaatgaa tttggtggaa atatagctgg
     7381 tgctaaaggt gtacctgtag atatgttaag aaaagcatct tctatggctg tttgtaaaat
     7441 aaacatggat actgacttaa gactagctat gactgctgct atcagaaaat tcttagctga
     7501 aaatccaaaa gaatttgacc caagaaaata cattggtgct ggtagagatg ctatacaagc
     7561 tgtagttgaa agcaagatag atgatgtttt aggttctgct aactctataa actaatataa
     7621 cttttaaata tttataaaag aaaagagatg acttaaagta attaaattta tttttaagcc
     7681 atctctttta agttgtataa attatgtatc ttataatatt ttaattatat tgtcttttta
     7741 aactgacagc taaacgtgaa tatgtccact ctttatcttt ctatggatgg tagaaggata
     7801 tatacctata acttcagcag cctttgttat tgaaccatac ttatttatag ccttacttat
     7861 cattatttgg tccactattt tatatgcatc attaagattc ataattccat ttaccactat
     7921 tggtgataaa tcatctgttt cattatcaat catatctaaa tttatcagca tgttaaattc
     7981 atcttctcca attacatttt tagctgataa tactacaaat ctttcaatga tgttttttaa
     8041 ttctcttatg tttcctggcc aagaatattc ttctaataac tcaataactt taggcgaaac
     8101 ttctacttct ctattgtact tttcattgta aagttttaag aaataattta ctaatggtac
     8161 tatatcctct tttctttctc taagaggagg tatttttact ggaataacac ttaatctata
     8221 gtacaaatct tgtctaaatt taaggttatt tctaaggtct tcatgagaaa tattagtagc
     8281 acttatatat ctaacatcta tttttatagg cttactacca cctattctag taatagtatt
     8341 ttcttgtata accctaagta attttttttg catctgcatt ggtaactcac ctatctcatc
     8401 caagaaaata gttccaccat tcgcctcttc aaaaagtccc tttttacctt ttttcttagc
     8461 tccagtaaaa gaaccttctt catatccaaa aaactctgac tctaataatt catttggtat
     8521 agctccacag tttatagcta taaagggttt gtctctcctt gaactatact tatatattgt
     8581 tcttgctatt atttcttttc ctacacctga ttctccattt ataaatatag aagaatctga
     8641 ggctgcagct ctcttaacaa ctttttttat attgtccata atcttacttt gacttatata
     8701 ctctgtgtct tctgatatat cactatattt tatctgagat tcaagcttaa taacccttga
     8761 aatatctctc cctgttagta ctcctcctgt aagctttccc ttagaatccc atcttggaac
     8821 tgccgtatat gcaataagct tcccatctgg gtaaacgata tttctataaa tctttttctt
     8881 cactttaagc atgtttaata ttattggttc ttcaactaat ccttgtctca caaggtctgt
     8941 tatatttcta ccaagtacct cagttctttt aaaaggtaat atctcatcac tgacagtgtt
     9001 catcttttca attaccccat ctttgttaaa cacaacaaca acttcatcta tatgctctat
     9061 tatatcttca attactttat cagatattag agacttctca aataaataaa ctatattatt
     9121 ttcattatta ttatctttca tattgtatat atgtgcagtc atccttaact ctctgaaagc
     9181 tattttcttt ttactatctg tcagtatttc tgttttgtca aaatttgaat ctaattcatt
     9241 tatatttcta ggctttttta tatctagaaa actaaaaata ctatctatta gatgattaca
     9301 gtatattatt tcgcctgatt ctttacataa aatggctgga ttttctacat gttctataag
     9361 ccctattaaa ttattatcca ttaatattcg cacctttcat taagtaattt attgaactac
     9421 atacaaaatt tattgtttgc ttacaataaa taccatcagc acatataatc aactccttat
     9481 aatattcgtc ttttcaagta gtttatttca ttaaaacgta actaagatat aattttaatg
     9541 attatagttt gttaaaaatt tcattttgct tttaattgta ttttatcaca tatttagtaa
     9601 ttatttgtct atttaataca atttaattaa atttaagatt tatttaaaaa aacaaagata
     9661 atccttacag aatataactc acaatttgta agcaatatac atataaggat tatccagatt
     9721 atcttgtatt ataaaatttt attcaaacag actttttaat aactgacaaa tacacactag
     9781 caataactta tcaaagggtt aataattatg ttactaacta ataagactta ttcaatcagt
     9841 aatatttata tagtttgtat ctattaagct tttatagctt ctaataaatc atcaacgcta
     9901 tctaattttt ctataagcat tggaactatt tcatgtaagt ctccaactac tccatagtca
     9961 gcgatttcta aaattggagc tgctgggttt ttgtttatag caattatgaa atctgatgat
    10021 tgcatacctg ctaaatgttg tattgctcct gatataccac aagctatata taggtttggt
    10081 ttaacagtag ttcctgtttg acctacttgg tgagaatggt ctatccatcc agcatcaaca
    10141 gccgcacgag aagaaccaac tactccacct aatttgtcag ctaatttctt aagcatttca
    10201 aatccttctg gtccacctaa tcctaaacca cctgatacta taacatttgc atctgtaaga
    10261 gatactaaat ctttcttagt tttaactgtt tctaaaacag tagttcttat atcatcttga
    10321 gttattttgt agtctaatgc tataacttca ccagttcttg tttcgtcttt ttcagcttta
    10381 tccataacac caggtctaac tgtagacatt tgaggtctat ggtttggaca aatgattgta
    10441 gccattatgt ttccaccaaa tgctggacga gtttgtttta ttttcttatc ttctgggtct
    10501 atttctaact tagtacagtc agctgttaat ccagttccaa ctcttgaagc tattctaggt
    10561 gctaagtctc tacctatatg agttgctccg aaaagcatta tttctggttt tatttcatct
    10621 attgcatctt ttattacttt tgtatatgca tcagttgtat atttttctaa aagagcatca
    10681 tctgcaacat aaactttgtc agctccaaaa gtgattaatt ctttagctaa tccatcaaca
    10741 tcttttccta ataatattgc acaaagttct gcatctactt ctttagctaa ttttcttcct
    10801 tctcctaata attctataac tactggagct atttttcctt ctctttgttc tgcaaatatc
    10861 catacgtttt tataagaact taaatctttt atatcattca tagtttgtac tcctccaact
    10921 tatataatgt gcttttgttt tagaccgatt attattttac tagctatttc atcagcttta
    10981 ccttctaaga tttctccttt tcctcttggt tcaggagtaa atgatctaaa tactttagtt
    11041 ggtgatgctt ttaaaccaac ttcttcttta tttacatcta aatcatcaat tgtccaaact
    11101 tttacatcat gtttatatgc ttttactatt ttatcaacag acatgtatct aggtgtattt
    11161 aactctttta ctgctgttaa aagtacaggt gtacttactt ttattaattc gtatccatct
    11221 tctaattgtc tttgaactat tatatctttt ccttctattt tgaaatcttg tacatatgta
    11281 acttgaggaa tgtctaattt ctctgcaatt tgtggtccaa cttgtgcagt atctccatct
    11341 attgcttgtc ttcctgcaaa tattatgtca tagtcaccta ctttagatat tcccgctgca
    11401 atagtatttg atgtagccca agtatcagct cctccaaatg ctctatcact taataatatt
    11461 gcatcatcag cacccatagc taaacactct cttagcataa agtctgcttg aggaggtccc
    11521 attgttataa ctgttatagt tacatcatca tgctcatctt taatttttaa agcttcctct
    11581 aatgcatttg catcatctgg gtttaatata cttggaactc catctcttat aagagtacct
    11641 gtttctttat ttattcttac ttcgttagta tctggaactt gttttacgca aactaatatt
    11701 ttcaaagtct atacctccaa tttcattaga ttatttatta ttttaatacg tttgatgaaa
    11761 ttaccatttt ttgaacttct gatgttcctt cgtatattga tacgattcta gcatctctgt
    11821 acattctttc gatttcataa tcttttataa atccatatcc accgtgaagt tgtaatgctt
    11881 tataagcaac ttcatttgca atttctgatg catagtattt agccatagca gattcttgag
    11941 caactctcat tcctgcatct ctcttttgtg ctgcatcata aactaatcct ctagctgcac
    12001 aaacttttgt ttccatgtca gctatagtaa attgagtatt ttggaattta gctataggct
    12061 taccaaattg aactctttgt tttgtatatt taacagcttc atctaaagca ccttgagcta
    12121 ttcctagagc taaggcagca acacctattc taccaacttc tagagtaccc attgctatct
    12181 taaatccttg accttctttt ccaagtaagt tttcttttgg aactttaacg ttttcaaata
    12241 ttaagtcaga agtttcagtt cctcttatac ccatcttatc ttcatgagct cctgttgaga
    12301 aaccatccca tttactttct actataaacg ctgatgttcc tctaagtcct ttacttctat
    12361 ctgtaactgc tattactata gctacatcac agaatggtcc attagttatg aaagtttttc
    12421 taccatttaa tatatagtgg tctccaacta attctgctgt tgtttgttgt ccacctgcat
    12481 cagaaccagc tcctggctca gttaaaccaa atgctcctat taattcacct gttgctatac
    12541 cttttaagta tttttgtttt tgctcttcag taccattgta aagtattggt aatgaagcaa
    12601 gagaagaacc agctgtaaca aaagtagctg ttgaagcaca tcttctagct atttcttcca
    12661 ttactatagc gtaagaaaca tatcctgctc ccccaccacc atattcttct gggatattac
    12721 tgcttataaa ttttgcatca gctaattttt tgattaattc cttaggcatt acacctgttt
    12781 tatccatttc tgctggtaaa gtatctaatt ctttactaac aaaatctctt actgcttttc
    12841 ttaaaagttc ttgttctttg ttatataaca tagcttcctc caattttcat attttatagt
    12901 aaatttattt taatgaactt acgctaaact catcatctca gcaaaagttt gaatacgagt
    12961 tctggcttgt tcattattct gagtttgttg gtcgatttca acatataaag taggtattcc
    13021 actatcttct atatcttttc taactaaagg ataatcgtat tcttctgggt cacagaattt
    13081 catcatacag aagataacac catctatatc tttcttttta acttcatcta ctataagtga
    13141 cccacgtttt ttcttagggt cataagctaa tgaacatcct tctatgtttg accattgtct
    13201 tgctaatctc tctaacgcat catcacctgc tggtacatct gttctgaatt gtcttgtttc
    13261 ttgtgctaag tcgtcagcta caactgatat attgttgtct tctaaaatgt ctaatatatc
    13321 tttagaatca gctaatatac ctgttaataa aactttcttt ccagaacaga cttcttctgg
    13381 catagcattt aattttgcta ttaaatcttt cactagctca gtatgttctt cttttctcat
    13441 aaagaaccca ctcttaatta ctaagcttct tattgatggt tttatagtat tagaatgttt
    13501 ataagctact tcaacaaagt ctctcatagt ttttctatgt tcattgtaaa cttctatact
    13561 ttcatgaatt tttgcttctt ctatttcata tccacaaatt tcttcaagtt ctctttttac
    13621 acctttgtac tcactgatta agtattttac accagcttca agttttctat tttgtgggtg
    13681 tactaatgat atatatttta tatgaggtac tgctgatttc cagttttgtc ctaaacaaat
    13741 tagtgtatca cacatacctg gtataataac tccagataat tcatcataag caccttttaa
    13801 tccatattct aaacatgatt gcattattga acatgcaaat gcagggaaat attgtttagc
    13861 taaatctaat tctgtttgtc ctccccatat accaactgga agcattccag ctgcatgtat
    13921 aatttcttct gggcaataaa ctgggaaaca acctatagct tttttaccag tttcactttt
    13981 atattttttt acagccgcat ttggattttc aactacttct ttcattttag ataaaatagc
    14041 ttccattata tctcacctct attaagtttt tttctttctt ccattacttc aactaaacct
    14101 tgaattcttg tttcaaattg agcattagtg aaagctctag ggtctgcttg gtcaccatca
    14161 aatccagcat atggtaatcc agtttcttca gctgctcttc tttgcatttc atattgtatt
    14221 aaactcataa gtttacagct tctgttcata tgatagaatg ctccatcaca tttaccctct
    14281 actaaagaat caactctata ttttgtcata cggtctaggt ttacattgtt aaacatagta
    14341 ctatatgcta ctgccattcc atctaaatca ttaacttcat attgtaatgc ccaagcatgt
    14401 gggtaaacac tacctgtcat gttaactcca aatttagcta atgttttcat cttgtatcct
    14461 atatatggcc aacaaggtat accttccatc attattctgt atttttcttc ccctctgaaa
    14521 gaagatttac cagttttcat gttgtcctct aattcttcta taagtaactt aaatgcttct
    14581 gtagtttctt ttttacctct agcacaaact attacagcca tgtaagtaaa taagtcaaat
    14641 ccattcattg gagaaggaga agaatctgct ggtaaactca tagaatactt ccatagtctt
    14701 ccgttttcag ctgatatttt cattacttct tcaaacttct tagggtcaaa tttctttcct
    14761 gatataattt ctagttgttt tatagcttct tcaaattgag ctttaatata atctattctt
    14821 gattgagtaa cttcgtcttc attattgaaa gttgtatcaa tcattattaa aggtatatct
    14881 aattctcttg aaatattttc ataccatttt ataacttggt tacatatatt gttacagcaa
    14941 agtaggaaat ctggagccgg catatccaaa gcttcacatc caccattttc taaaagacca
    15001 aaatttgttc ttgcatatgc acataggtca atagaatatc ctttagattc agctatttca
    15061 cataattcta aagaaccttt tttagctgct actccagctg cttggttttc tggatataat
    15121 acgtttaagt caaatacttc tgctaactct tgaggaaata ctgatgttga ccaacctaca
    15181 ggtcttcctt cttctttagc tttaaatgca tttgcatatt gttcagctaa taaatcatta
    15241 attactactc tagcttcttt tttttcagac attttcatcc ctctttttat atatttttca
    15301 cttctttttg tgattctttt gcttcatcaa aagcatataa cgctgctcct aaagcaccag
    15361 ttaattgagg tatatcaggt acaataattt ctgtgttgat ttctcttgcc atagctctta
    15421 caataccact atttctagca actccaccaa ccataactac atttctttgt actcctattc
    15481 tttttactag gctagaaact ctctttgcta ctgaagtatg aatacctgcc actatatctt
    15541 caatttttgc attttcagat aaatgtgata taacctcaga ctctgcaaat actgtacatg
    15601 tactgcttat tgatacttca ttttgagaat tcatagatat acttccgagt tcagatacat
    15661 caacctctat tatttttgcc attacatcta aaaatcttcc tgtacctgca gcacatttgt
    15721 cattcataag aaagtttaat agtcttccat tattatctaa ttttaatacc tttgcatctt
    15781 gaccacctat gtcaataatg gttcttgtct ctggaattat gaaatttacc cctctagcat
    15841 gacagcttaa ttcacttatt tgcttatcag catctgaata gttcattctt ccatatcctg
    15901 taactacaac ttttttaata tcttctcttg caagacctgt cttgccatat aatttttcta
    15961 aaactcttga tggtccagta gtcccagtac cagaggatat tgtttcagaa gctacaatat
    16021 cttccccatt ctttaagatt actccctttg atgcagttga acctatatct aatcccattg
    16081 tgtacatctt tagcattctc cttttaaaaa atctagaatt actaattaat atcttacaac
    16141 tttactatct ttaaagttat ttattttctc ttcatcgtag cctaaagatt ttaatacttc
    16201 tacagtatgt tgacctactt ttggtgctgg tgtatattct ttaattcctt catttctaaa
    16261 cataactgga gtattaacta agacacctgt atttccgcta tcgtatgttt tcttaaataa
    16321 gaagtcattt gcccaagctt gttcgtcatc taataaatct tcacagcttt gaattttttc
    16381 aaatggtaag tctgcttctt ctaataaagc tgaccactcg tctaatgttt tttctaacat
    16441 agcttctcca actatcttaa ctaaatcttc aacatgatta accattgaat ctatgttatt
    16501 atatctatcg tcttctaata tatattctct atttataacc ttacagaatt tgcctaacca
    16561 cttgttgtat tgtattaaag ctaattgaat ccatcttcca tctttacatt tatatgtagt
    16621 cattaatggg ctgtttggat tttctcttga taaaggcatt tcatttccgt attgtgctgt
    16681 tgttatcatt gttcccattc catatatagc tgtatggaaa agacttactg ttactctctc
    16741 acctttacca gtttgagctt ttttatgtaa tgctgctaaa cttcctgctg ctagtgctag
    16801 acctgcatag tggtcaccaa atcctgctgc tgtatttgct ggagatgttc ctttttccat
    16861 aacagattgg ctaactcctc ctcttgcgaa gtatgcagta tagtcaaatc ctggtttatc
    16921 ttttaaaggt cctttttcac catatcctaa tatttgagag aatattaatc ctggatactt
    16981 atcttttatt tggtcataag ctatacccat tttttctaat gcttgaactc taacattagt
    17041 tacaaatatg tctgcttctg ataataattt atgtaatatt tctactcctt cttttgattt
    17101 tacattaata cttacaccct ttttatttcc attttctaat tcaaacatag ggttttcatc
    17161 atctgatgct ggagatttaa atgttccacc cataactctt attccatcac cttctatagg
    17221 ttcaatctta ataacctctg caccccagtc acctaacatt tttgcacaac atggtgctgc
    17281 gatgaaactt gaaagttcta ctactttaac tccttctaaa agcataacgt tttcctcctt
    17341 aatagtatca attataaaat ttcgattaaa tcactcataa aatacttttt tctgatatta
    17401 actgtctgat gtcaaagttc ctaatcttat ctaaaataac ttaaaaatta ttaaaattat
    17461 tttttattgt tttattgtct tacacctata ttgttaagca tttatcgtgc caactttata
    17521 tttaatcaaa atatcgcttg atttgaatgt tttttaacaa tatgcaattg aataatcttt
    17581 ttcatatcgt ggcatcttgc cactatccat tgcattttgc tattctttct atgcttataa
    17641 aagttttttt tatatatttt tttatgaaaa agcttgacat ttcaaaaaaa gttttcattg
    17701 ctaaactcaa aaacacatgt tatcattgta attaatcagc aagttagtta actaatttca
    17761 aacttagata attttttaat aatatttaat ctaaaaaata acaatttctt tgataaattt
    17821 tagagtaatt attacattaa ataaaaaatt actaaatatc aaaataacac agggaaaaca
    17881 atttcaaaac taaattttta accttaaaaa ttttgtataa aaaatataac cttatatgct
    17941 acatattatt tctaaatgaa aaattaccat aataatttaa taattacagg agggtattga
    18001 tatgaaaata ctagtatttg gagcacgcga ttatgaagaa ccagtaataa aaaaatggtc
    18061 tgaagaacat aaggatgttc aagtggatat ttatcctgaa aacatgactg aagaaaatgt
    18121 agttaaagct aaagggtatg atggtatatc tatacaacaa actaactata tagataatcc
    18181 ttatatttat gaaactttaa aagatgctgg ggttaaagtt atagcttcaa gaactgcagg
    18241 ggttgacatg atacattttg atttagttaa tgaaaatgga cttatcgtta caaacgttcc
    18301 ttcttattca cctaatgcaa tagctgaatt agctgttact caagctatga accttttaag
    18361 aaagactcct ctagtaaaga aaaaagtctg tgaaggtgat taccgttgga tagctgaact
    18421 tcttggaaca gaagttagat ctattacagt tggtgttata ggtacaggaa aaataggtgc
    18481 tacttctgca aaattattca aaggcctagg agctaatgta attgcatttg accaatatcc
    18541 aaatagtgat ttaaatgata tattaactta caaagattct ttagaagacc ttctaaaaga
    18601 agctgacctt ataacattac atactccttt acttgaagga acaaaacata tgataaataa
    18661 agatactcta gctataatga aggatggagc ttacatagta aatactggcc gtggtggttt
    18721 aattaataca ggggatttaa tagaagcact agagtcagga aaaattagag ctgctgccct
    18781 tgatacattt gaaactgaag gattgttctt aaacaaaaaa atgaatcctg gagaattaac
    18841 tgacccagaa ataaataaac ttctttctat ggaacaagtt atattcactc atcaccttgg
    18901 tttcttcact agtacagcga ttgaaaatat agtttattct agtttaagta gtgctgtaga
    18961 agttataaaa acaggaactg ctactaatag agtaaattag tattttataa atatattttt
    19021 tcattaatta gctattatct aacaaaatag cctttaattt cccaaatata agagagtcag
    19081 ctcagtatat attgagtaga ctctcttata ttatttttta tattaaactt gtactcttct
    19141 tattcatacc aaattaaatt tatattaact tactctaact actcctactc tatacatttt
    19201 acctattttt acttttctaa ttatatacat ttgaatgggt acaatctgta ttttacaaaa
    19261 aaaacaccta acaaactatg aaaattgtat gattaatatt aatatattta taagaaaaca
    19321 taacaattat gttagctctg gatttttagg gagggagtat attggaaaaa atgaataaag
    19381 ttacttctaa aaaaactacc tatattgtta cttcagcatt atttgcatcc ataatatgcc
    19441 tgacaatagc atatatatta catattccag ttggtggaaa caatggatat gttcatatag
    19501 gagatgcttt tatttatcta gctgcaacta ttttgccaac aaactatgct atagctgctt
    19561 ctgcaattgg tgctggtcta gctgatttat ctacaggtgc tgctatctgg gtaataccaa
    19621 ctataatcat aaaaccaata cttgtattat ttttcacttc taaaagtgac aagataataa
    19681 ataagagaaa tatagttgct tcggttgtag caggtatagt aggattagtt ttatacatgt
    19741 ttgctgaagg tataattatt ggaagtttta caagtgcatt tgtaatgagc ttacttggct
    19801 tattacagcc aataggaagt tttattgttt ttataatctt aggtatggca cttgataaac
    19861 ttgattttaa aaaacgttat tttaattaag aatttaaatt tatggaggga aaattatgaa
    19921 tatcttatat acaattgaaa atagtatcta tgtaaatata actaatacat gtccatgtag
    19981 ttgtgttttt tgtataagaa acgaaaaaga tgaagttgca aatagtggta gtttatggtt
    20041 ggagcatgag ccaagtgtag atgaagtaaa agaagccttc aataaataca atttagacga
    20101 ctatgatgaa atcgtatttt gtggatatgg cgaaccatta atgagaataa atgaattgat
    20161 agaggtagca aagtttataa aagaaaaaag tagcataaag ataagaataa atacaaatgg
    20221 tctaagtgat ttaatacata acaaaaaaac agctattctg ttaaaaaatg ttatagatgc
    20281 agtatcaata agtcttaatg ccccaaataa agaagcttat aatagagtta ctcaaccaaa
    20341 gtttggagaa aaatcatttg attatatgtt agattttgca agagattgca aaaaatatat
    20401 aaaagaagta gctttttcag tagtagatga aatatctcca gaagaaatag aagaatctaa
    20461 acaactggca aaaaaacttg atataccatt aagggttaga cataagaatt agcaagtaaa
    20521 attataaaac attaagaatc acctgaatgg tctttcaggt gattcttcct cttgtattaa
    20581 cattttacaa aatagatttc ataccacact aggaatgtgt aaatcatcca aatctttctc
    20641 caattatcag agtttccact aatgtagtca ttaaaaatct cattaatctc atctatgtta
    20701 aagaatttat ctgctatctc actattgaat ttatctcgga tatccttatt atattggtca
    20761 tcagccatcc atatacgaat tggcacaata aatcctaatt tatttctaaa agcaatctct
    20821 tctggtaatg tttttgatgc agctgtacga aaggcaattt tattctcttt tcctttcact
    20881 ttatattctg atggtattct ggatgcaata tcaaatattc ttgtatctgt aagaggcatt
    20941 ctgacttcca atcctactgc agtactcatt ttatctatat taaaataaat atctccttcc
    21001 aaccatattt gaatatctac atttgacatg ttagtaagtg tatctagttc ttctgtttct
    21061 tcataaattc cttttaccac ttcaatagga agcacatcag gattgtattt cttaagaatt
    21121 tttcttttct caccctcatt cataatgttt gtatttccaa tataaaatgt cttcaaattc
    21181 tctccataac actctgcctt atggtatata ttatatccac caaaaaactc atctgctcct
    21241 tctccagagt aacagatatt tgtgttttca gctgctgcct tgcatccaat agcaaaaacg
    21301 atagcagatg catctccagt tggttgctcc atatggtaca ttacataagg tcctgacgca
    21361 aaatactctt caggtgtaat tatatatctc gaatttttac acccaagaat attagctgtc
    21421 tgttgtgcta attttgattc atcaaaacgc tcttcctcat aaccacatga acctgttttt
    21481 tctatatctg acattgccaa tatataagat gaatcaacac ctcctgatag gaataactct
    21541 gcagtttcat tctctgattt tacctctggc ataattttct gaagtgttgt atgaatttca
    21601 tccgtccatt cctcaataga cttgctgtgg tcaggatgaa attcaggttt ccaataacga
    21661 ccaatacata ctgttttatt ctgatagaca aggtaatgtc ctggcataag cttctttact
    21721 ccacggaaga atgtattttc acctgctaca tatgtgaaag acatataaat ctgtaacatg
    21781 tcttcattta actctttcac aaatcctggt tgctccataa tcttacgaat agatgaacca
    21841 tagagaagct tcccattttc agtctcataa taatagaaag gttttacccc aaactggtct
    21901 cttaggcaga agagtttctt ttccacagta tcccataatg caaaagcaaa cattccatgc
    21961 atgtgatttg ccatatcata tccccatgtc ttatatgctt ccactaaaat tgctcgttca
    22021 cgctcatctc tacataatga agaatcgatt ccaagttctt cacaaagaac tttccagttt
    22081 cgaatatgcc cattgtattc tagaattttt atcatatatg tgtcttttcg tgttaaatac
    22141 ttttcttcta aatttatcat atgtgtatcc tttcgtatta aaacatttaa cttctaaatt
    22201 tattaaatgt ttatatatat ttattcactt ataatcactg aaaaatcgac ttttttcaaa
    22261 gtaaattctt tttgaattag tactaatctg ctctttatta atggcataaa tatgattatc
    22321 aagtattatc agtgttataa cattgaattt tttattcttc ataaatattg cc
//
LOCUS       DACULQ010000049        22370 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.049, whole genome shotgun sequence.
ACCESSION   DACULQ010000049 DACULQ010000000
VERSION     DACULQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.049"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="JA154_07650"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="JA154_07650"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            626..1696
                     /locus_tag="JA154_07655"
     CDS             626..1696
                     /locus_tag="JA154_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7995739.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1680..2549
                     /locus_tag="JA154_07660"
     CDS             1680..2549
                     /locus_tag="JA154_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAT7995740.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2593..3453
                     /locus_tag="JA154_07665"
     CDS             2593..3453
                     /locus_tag="JA154_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAT7995741.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3484..4788
                     /locus_tag="JA154_07670"
     CDS             3484..4788
                     /locus_tag="JA154_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7995742.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4851..6113
                     /locus_tag="JA154_07675"
     CDS             4851..6113
                     /locus_tag="JA154_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HAT7995743.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6300..8270
                     /locus_tag="JA154_07680"
     CDS             6300..8270
                     /locus_tag="JA154_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089932.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. The beta-hairpin of the
                     Uvr-B subunit is inserted between the strands, where it
                     probes for the presence of a lesion; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAT7995744.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8289..11114
                     /locus_tag="JA154_07685"
     CDS             8289..11114
                     /locus_tag="JA154_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAT7995745.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11373..13190
                     /locus_tag="JA154_07690"
     CDS             11373..13190
                     /locus_tag="JA154_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HAT7995746.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13190..14119
                     /locus_tag="JA154_07695"
     CDS             13190..14119
                     /locus_tag="JA154_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAT7995747.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14333..16129
                     /locus_tag="JA154_07700"
     CDS             14333..16129
                     /locus_tag="JA154_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HAT7995748.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16269..18050)
                     /locus_tag="JA154_07705"
     CDS             complement(16269..18050)
                     /locus_tag="JA154_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HAT7995749.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(18073..19959)
                     /locus_tag="JA154_07710"
     CDS             complement(18073..19959)
                     /locus_tag="JA154_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HAT7995750.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19976..20473)
                     /locus_tag="JA154_07715"
     CDS             complement(19976..20473)
                     /locus_tag="JA154_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HAT7995751.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20791..22272)
                     /locus_tag="JA154_07720"
     CDS             complement(20791..22272)
                     /locus_tag="JA154_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAT7995752.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8604 a         3000 c         3738 g         7028 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc attccgaaca cagaagttaa gctctctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg taattttttt
      181 tgggtaaatt tataaaaatt aaaaaatttt tattttttat gtttaaacct tgtaaatttg
      241 ggacataata taattgtaaa acttaatata gcaaaaataa attactcaat ctcccccact
      301 taaagacctc tatcccccat agaggtcttt tttactttca aatcaaatta tataaaaaat
      361 aaatagtttt atatgactct tcataaatta aaattacatt gtgatagcaa acaatgaata
      421 tgaaaaatat agaaaattat agctaacata agctgtatat catcttaacc tcagtatatt
      481 taacaaaaaa tgtttataat ttaaccttta ttttaataag tttaccatat attcactttg
      541 tcttaatata tcctttacaa attaagagta ttattaatct ataagtaaat cgtagaatta
      601 aattaaatat tttgggagga taaaaatggc agatataaga ctagaaaatg taacaaaatc
      661 ttataataaa aaaaatacag ttatagaaaa tttaaatctt actatagaag atggttcttt
      721 tacagtattg gtaggcccaa gtggatgtgg taaatctact acgttaagaa tgattgcagg
      781 tcttgaagat ataacttcag gtaggttgaa gattgaagaa aatgatgtta caaaaacgga
      841 tgctagtaaa agggatatag ctatggtatt tcaaaattat gcattatatc cacatatgac
      901 tgttagagaa aacatagaat ttggtcttaa aaacaaaaaa gtagaaaaag aaaaaagaga
      961 aaaacttata acagaagtta ttgatgtagt aggattgaga gaatatttaa atgtgaaacc
     1021 aggcaactta tctggaggtc aaagacaaag agtggcactt gctagagcta tggttaaaaa
     1081 tccaaaggta tttttaatgg atgaaccatt gtcaaattta gatgctaaat taagaaatca
     1141 aatgagaaca gaattaatac aattacatca aaaactaaag tctacatttg tttatgtaac
     1201 tcatgaccag gttgaggcaa tgtcaatggc agataagata gttataatga acaaaggaaa
     1261 gataatgcaa ataggttctc caaagaagat atatcaaaat cctaaaaatt tatttgtagc
     1321 tcaatttata gggagtcctt gtatgaacat actagatata aaaaatgata ttaaatttgg
     1381 attcagacca gaaaaagcaa cactagagag tataaacata gatggaaaaa atgatgatgt
     1441 atttagtcta tttggaaaag ttgtaactaa agaagtgttg ggaaatgaaa ctatatattg
     1501 tttatctgtt cttaatagag agataagagt caaaacagaa aatgatatat ttgatattgg
     1561 aagaacttta agaataagtg tattagaaaa agatttatat tattttgata aaaatggaga
     1621 aagaatagaa gatgtaaaag tatgtaaaag tatgtattct aaaattaaag gagatattta
     1681 tgaaaaagca atttaataaa gcatatttat atataacacc tgctattata ggaactttat
     1741 tgtttatagt ctatccaata attaagacga tatctctaag cttcaaatct ggtagtcttc
     1801 ttagttcaac ctttgaaaat gtaggcttaa acaattatac agaactttta aagaatccag
     1861 agtttataca aactataaca aacactgcaa tttatacttt ttttatggtg actatatcta
     1921 tatcactagc catagtactt gctgtttggc taaataaaaa ttctataata cacaatttaa
     1981 cgcaaagtat aatctttaca ccacatgtaa tttcactagt atctgtagct gtattgtggc
     2041 tttggattat ggagccacaa tatggctttt taaactggat actatcgaca ttacatttac
     2101 caacatcaaa gtggctttca agtgaaagtt cagctttaat gtcactaatt ctaataggaa
     2161 tatggaagac tcttggatat aatatcttaa tagtgttatc aggtcttaaa tccataccaa
     2221 actatatata tgaagcatct aaattagata atgcaaacaa aataacgaat ttctttaaag
     2281 tgacattgcc actattatca ccaacaatat tttttcttat gataacaaca acaactgcat
     2341 cttttcaagt atttgatgag ataagtataa tgacacaagg tgggccagtg ggttcaacta
     2401 acatgttatc ttattatata tatgagtctg gatttatata ctatgacata gggcaagcga
     2461 gtgcagcgag tatgtttttg ttagctatag ttatgattgc tacatatgct aactttagac
     2521 tcttatcaaa gaaagttcat tatcaataaa gaaaaagtag atattataag gtttatccaa
     2581 aaggaggaga caatggatat tatacaacct atcaaaaaag acaaagttga tataaaaaaa
     2641 atagcatcaa aatcaataga aatattaatt ctattacttt tatctgcaat atttatattc
     2701 ccttttatat ggatgctatc tacatccttt aaaaatccac aagaaatgat gcaattaccg
     2761 cccactatta taccaaatga tatagtgtta gaaaattata gtacagcatg gcattcaggt
     2821 ccatttttta gatatttgct aaacagtgta tttgtaacag gaagtgtttt aattgtacaa
     2881 tttttagtta tggttccagc agcatatgca ttttctaaga ttaaatttaa aggtgaaaaa
     2941 atgctatttg ggcttttgct tgtaggactt atgattccac cacaagtaac ttttctacca
     3001 gtctatatga tgatgagtaa gtttggattt ataaatactt atgttccact tataattcca
     3061 tttatgacat cttcatttgg gatatttctg cttagacaga attttatgca gatttcagat
     3121 gaaattattg aagcagctaa actagataat gcttctgact taaaaataat gtttagaata
     3181 atggttccta tggcaaaacc agcagtaata acatttatat tatttaattt tatataccat
     3241 tggaataatt atttctggcc cttagtaatg acaaatacag atgcaataag aactttacca
     3301 ataggagttg cgctccttaa atcttcagaa ggaattactg catggaatgt aataatggca
     3361 ggaaatataa ttctaatttt accaataatt cttgtttata tatttgcgaa taaaaaagta
     3421 aaagaagctt ttatgtattc aggaataaaa taaaaaatta aagactaaat ttggaggatg
     3481 aaaatgaagc ttaaaagaaa attagcaaca ggattagcaa taacattatt attaggttct
     3541 ttagttgggt gttcaaattc taatgatgca aatgcatcta aaggagaaaa gactgatagc
     3601 aatattacga agatagattt ctgggctcct ttaggtggaa ctaatggaga aactgctcaa
     3661 aaaatggttg accaatttaa tagtgaacat aaggatgtac aagtaaatat gttaaaacag
     3721 aaagattact atgaaaatgc aacaaaacta caggcagcct taacatctaa agaccaacca
     3781 gatgtaacat tattagaaat aactcaaaca ggtacattcg catcagcagg tgctttagta
     3841 gatatgagta aatattttga taaaacatat caagaaagat tcttttctgg tcttttaaca
     3901 aattcatatt atgaggataa gtttgttggt atgccattta atagaagtac accaatatta
     3961 tacataaata aagatatggc aactaaagca ggtcttgacc cttcaggtcc aaaatcatgg
     4021 gaagagctta aggattatgc tagtaaaatg acaaataaat cagaagatac ttatggtttt
     4081 gaaacaccaa tagatatttg gttctatgaa gctatggtaa tgcaaagtgg aggagaaata
     4141 actaatggta aaaaagtagc atttaataat gaggcaggac aagcaccagt taagttctgg
     4201 caagatatga tgaaagctgg aataatgaaa atgcctcctg gagaagatta taatgcctgg
     4261 gacgtagcaa aacaagattt tgtaaatggt aaagtaggta tgatatttac atcaacagca
     4321 gatttagcag gacttatgca acaaacagaa ggtaagtttg aaatatcaac agcattctta
     4381 ccaaaaaatc aaaaatacgc aactcctaca ggtggagcta atctagttat gcttgaaggt
     4441 ggaacagata aagaaaaaga agcaagtgca gaatttatag aatggatgac acaaacagat
     4501 aaaatcgttc agtttagttc atcaacagga tacttaccaa caactgaaga tgcaacaaac
     4561 tcagaaaagt tacaaacatt atataaagaa aaacctcaat ataaggttgc aactgaccaa
     4621 ttacaatatg ctgttgcact tccaatgtta aatggataca aggaagcaac tgataagcta
     4681 atggatgaga ttaaaaaggg gcttactgat ttaaatgcaa atccaaaaga tgtagtaagc
     4741 aaaggaactt ctgcaatgca agcagtaata gataaaacaa ataaataatt atatagactt
     4801 gtaagttgat taaaagtatt caataatata taaatgaaga ggtaatgtaa atgacaaagg
     4861 ttatagtatt atcagtagat tctttatttg aaaaagactt ggaatttgta aaaaatctac
     4921 caaactttaa atctatacta gaaaactgct ccatagtaaa aaatataaat tgtgtttatc
     4981 ccactctgac atatccgtgt catactagta tggttacagg agtatatcct aaaaaacatg
     5041 gtatatatca taatgaaaaa tttgacccaa ataaagaaaa taaagactgg tactggtact
     5101 caaaggacat aaaagcaaaa actattatag atgtagcaaa agaaaataat ctaactacct
     5161 ctagtgtatt atggcctgta atgggagcaa atccaaatat agattataat attgcagaaa
     5221 tatgggctcc tagtagagaa gatgacccaa gagaaacttt tgaacgaagt tccagtaagg
     5281 ttataatgga cggtatttac aatagacatt gtcattatat cgattggaaa ttagaaccca
     5341 acatggattt atttggagta aactgttcag tagatataat aaaggaatat aaaccagatt
     5401 taatgctaat acatttagct acattggacc atacacgaca caattatggc ttatttaatg
     5461 acgaagtaga taaagcatta aaaatgaatg ataaatggtt aggagatatt attcaggcta
     5521 caaaggatgc aggtacatac aaagatacaa attttataat acttggggac catggtcatc
     5581 ttagagttga taaagttata aatccaaatg tcttgcttaa atcaaatgga tttataaaag
     5641 ttgaaaatgg agaagttaag gattttgatg cctatgtaaa ttcttctgga atatcagctc
     5701 aaataatagt aaataactta gataggttaa cagaattaag agaactgctt gaagaattta
     5761 aagaagaatt attcatagag aatattttta ccaaagagga agcatctaac cttggtcttg
     5821 aaggtgattt tgaaatggta attgaaggtc ttgaaggaat ttcatttgga aatgatttcg
     5881 aaggaagtat tataaaagat tcagatataa aagattacaa gtttgctgtt gcgacacatg
     5941 gtcatttacc tactaaaggt aatagacctc catttatagc ttttggacca aatattaaag
     6001 gtggtgtggt tatagaagaa ggagacctga gagcacatgc agcaactata ttaaaaatgt
     6061 ttaatttaaa attagatgga gttgaaaaag aagcatttga ttttattatg taagttgaat
     6121 aaagtattta ttttccatga attatagact tctattgcaa agatagaagt ctttttttat
     6181 gttttagatt taagtaaaat ttaaatgggt aaaatattta aaataactta aacaatttta
     6241 taaaggtatg atataataat acatgtcagg aactaatgtt cgaaaaaagg agtgatacta
     6301 tggattttaa aataaaatca gacttcaaac ctacaggtga ccaaccagaa gctattaaat
     6361 caatagtgga ttctataaat agaaatgaga aattttcgac attattaggt gtaacgggtt
     6421 caggaaaaac attcacaatg gcaaatatca ttcaacaagt taagaaacct accctaataa
     6481 tggctcataa taaaactcta gcagcacaac tttacagtga gtttaaagaa tttttcccag
     6541 acaatgcagt tgaatacttt gtaagttact atgattatta tcaaccagaa gcatatgtag
     6601 ctcacagtga cacttatata gaaaaagatg caagcataaa tgatgaaata gataaacttc
     6661 gacattctgc tacagcatct attttagaga gaagagatac tataataata tcatctgtat
     6721 cttgtatata tggacttggt gacccaaaag actataaaga acttatgtta tctataagac
     6781 caggtatgca aagagacaga gatgatgtaa taaaaaggtt aatagagata caatacgaaa
     6841 gaaatgacat aaactttaca cgaggtacct ttagagttag aggagatata ctagaaatat
     6901 tcccagctag taatgatgaa aaagcaataa gaatagaatt ttttggtgat gaggtagata
     6961 gaataacaga aattgattat gtaacaggga agattgttgg tactagaaat catgttgtta
     7021 tattcccagc atctcactat gtaacaacgc cagaaagaat tgaaaaggca attgtagaaa
     7081 tagaaaacga gcttcaagaa caaattaaat ttttcaagga aaatgatagg ctattagaag
     7141 cacaaagaat tgaacaaaga actaagtatg atatagaaat gttaaaggaa ataggatttt
     7201 gtcaggggat agagaactat tctagacata ttacaggaag gtcagaagga gaaagaccat
     7261 atactttaat ggatttcttc ccagatgatt atttgataat tgtagatgag gcacatgtca
     7321 ctataccaca agtaagaggt atgtatgcag gagacaggtc tagaaaaaca tcacttatag
     7381 agaatggttt tagattacct tctgcacttg acaatagacc attaaatttt caagaatttg
     7441 aaggaaatat aaatcaaatg ttgtttgtaa gtgctacacc agggccttat gaaattcaac
     7501 actcagagac aatagcagaa cagataataa gaccaactgg ccttttagac ccaattgtag
     7561 aagtaagacc tataaataat caaatagatg atttggtagg agaaataact aaaactatag
     7621 aaaaaaatga aagagtgctt ataaccactt taactaaaaa aatgagtgag gacttgacta
     7681 actatcttaa agaaataggg ataaaagtta aatatttaca ctcagatata gttacattag
     7741 aaagaacaga aataataaga gatttaagat tagggaagtt tgatgtcctt gtaggaataa
     7801 acttacttag agaaggattg gatataccag aagtttcact tatagcaatt ttagatgcag
     7861 acaaagaagg tttcttacgt tccgaaacag ctttaataca gactattgga cgtgcagcta
     7921 gaaatgaaaa tggaagagta attatgtatg cagacaggat aactgactca atgcaaaatg
     7981 ctattgatga aacaaagaga agaagagata tacaaaattt atataatgaa gagcacaata
     8041 tcattcctaa aactatacag aaaaatataa gagatagtat agaagctact aaggttgcag
     8101 aggaagaagt tgtatatgga atatctgata cagatgataa agacgaaata agagctaata
     8161 tagataaatt aaaatcagag atgatggaag ctgctcaaaa cttacaattt gaaagagcag
     8221 cagaacttag agataaagtg aaacaattag aagaaaaatt ggaaaaatag aaaggtaaaa
     8281 acaacattat ggaagataaa attataataa gaggagcaaa agaacataat ttaaaaaata
     8341 tagatttaga attaccaaga gataaattta ttgtttttac aggtttgtca gggtctggga
     8401 agtcttcttt ggcatttgat acaatatatg cagaaggaca gagaagatat gtagaaagtc
     8461 tatctgctta tgcaagacaa tttttaggtc agatggaaaa gccaaatgta gaatatatag
     8521 agggtttatc accagctatc tcaattgacc agaagactac ctcaaaaaac ccacgttcta
     8581 cggttggaac agtaacagaa atatatgatt atttgagact attatttgca agagtaggag
     8641 atgtgtattg ccctacatgt ggtgaaaaaa taagtcagat gactattcaa gaaatagttg
     8701 ataaaatgct agattttcca gatagaacga aattacaaat tctttcacca attgttagag
     8761 gacaaaaagg tacacataaa aaagtctttg ataatatcaa aaaagaagga tttgtaagag
     8821 ttagagttaa tggtgaaaac tatgaagtta gtgatgatat aatcttaaat aaaaatcaaa
     8881 aacataatat agaagttgta gtggacagaa tagtaattaa agatggaata gaatcaagac
     8941 tagcagattc aattgaaact gcagttaagc tatctgatgg tcttgtaata gcccaaataa
     9001 tagatggaga agaaattctt ttttcaacta aatttgcatg cccagaacat ggaattggca
     9061 tagaagaatt atctccaaga atgttttcat ttaatgctcc ctttggtgct tgtgatgttt
     9121 gtaatggtct tggggaaagt aaagaagttg acccagaatt agtagtacca aacaaagatt
     9181 tatcaataaa acaaggtgca gtggcagcat ggggttcaac aggtgttaat gatgacactt
     9241 attacagtaa aatgataaaa agtttagcag agcattttaa tgtatctttg acaacaccgt
     9301 ttaaagattt accagatgat tttgtggagg aattacttta tggtaaagat aatataattg
     9361 tagagtttac ttatgagtca aaatttggag gaactagaaa ttataaatca tactttgaag
     9421 gtgtaatagt aaacttagag agaagatata gagaaactaa ttcagattac atgagagata
     9481 agattgaaga atacatggca gagagaccat gtccaaagtg taaagggatg agacttaaaa
     9541 aggaagtttt atctgtttta gttgatggaa aaaatataat ggaagttaca aacttatctg
     9601 taaatgaatt gattgacttt atggaaaata taaatcttac agaaaaacag agatttatag
     9661 ctcatgagat tataaaagag ataaaaggaa gagctatgtt cttaagagat gtaggtcttg
     9721 attatttaaa tttatcaaga aaagctggaa ctttatcagg aggagaagcg caacgtataa
     9781 gattagctac tcaaataggt tctgcattgg ttggagtttt atatgtactt gatgagccaa
     9841 gtatagggct tcaccaaaga gataatgata ggcttataac tactcttaga catctaacag
     9901 atttaggaaa taccttaatc gttgtagagc atgatgaaga cacaatgaga gaagctgatt
     9961 atgttataga cattggtcca ggtgcaggta tacatggtgg tgaaatagtt gcacagggta
    10021 ctttagatga aataattgaa aatgagaact caataacggg tcaatattta agtggtaaaa
    10081 aacaaatatt gcttcctgag actactagag aaggaaatgg aagttttata gaaatagtta
    10141 aagcatcaga aaataatctg aaaaatattg atgtaaagtt cccattaggt aagtttacat
    10201 gcataacagg tgtttcaggt tctggaaaaa gtacattaat aaacgatata ctttacaaag
    10261 gtgttgcaag taaagtgaat agacttaagc aaagagctgg taagcataag gagataaaag
    10321 gaattgaaag tatagataaa gttataaata tagaccaaag tcctattgga agaacacctc
    10381 gttcaaatcc tgctacttat acaggtgtat ttgattttat aagagatatt tttgcatcta
    10441 ctaatgaagc taaagcaaga ggttataaaa aaggtagatt tagttttaat gtaaaaggtg
    10501 gtagatgtga agcttgtaag ggtgatggaa taataaaaat agaaatgcat ttcttaccag
    10561 atgtatatgt tccttgtgaa atttgtaaag gtgaaagata caatagagaa acattgcaag
    10621 taaaatacaa agataaaact atatctgata tattagatat gaatgtggaa gaagctgttg
    10681 agtttttcga aaatatacca aatataaaaa gaaaattaga aactttaatg gatgtaggac
    10741 tttcatatat taaattagga cagccttcaa ctcaattatc aggaggagaa gcacaacgta
    10801 taaaattagc tgctgaactt agtaaaagac caacaggaaa gactttgtat atattagatg
    10861 aacctactac tggacttcat atggcagatg tagacaagct aatccaagta ttacaaagac
    10921 tagcagatac aggaaataca attatagtca tagaacataa tttagatgtt ataaagactt
    10981 gtgattatgt aatagactta ggaccagaag gcggagataa gggtggaact atagttgcaa
    11041 ctggaacacc aaaagaagta tctaaagttg atggttctta tacaggtcaa ttcttaaaga
    11101 aatatttttc ttaagttata taaattgtga aaaattttag agcagatata cgataaactg
    11161 ttttgttata agtaaattag tcaaatataa gttgtaaata gtaatacaaa gtaaagtcca
    11221 tattactctt catggttaaa attgttttca aatcaattat agaagggaat atgggcttta
    11281 acttgtttaa agcactggaa attgtataac tttttaaaat tcatatttta gggtataatt
    11341 ttagtataaa atttaatgtt ttggagggcg agatgtttga tatacaagag catttaaaaa
    11401 aattaccttc tgagcccgga gtatatttaa tgaaagacaa atacgaccat ataatatatg
    11461 tgggaaaagc catatctcta aaaaataggg taaggcagta ttttcaatct tcaaaaaacc
    11521 atacatctaa agtaaaatct atggttaaga atatatataa gtttgagtat attataacgg
    11581 attcagagtt agaggcatta atacttgaat gtaatttaat aaaaagatac agaccaaagt
    11641 ataatgtagt gcttagagat gataaaactt atccatatat aaaagtaact acgaatgaag
    11701 attatccaag aatcttaaaa gttagaagag ttcttaaaga taaggcaaag tattttgggc
    11761 cctataccaa tataactgct gtgaatgata ccttggagct tataagtagt acgtatccaa
    11821 taagaagctg taaaatagat atagataaag caataaagaa taagacaaga ccttgtttaa
    11881 atctacacat aaataaatgt ttaggacctt gtacaggaaa tgtttctaaa gaagagtatg
    11941 gaaaaatgat agaagagatt ataatgtgtt tatctggtaa agaagaaaaa ttgatggaac
    12001 ttttaaagga aaaaatgaat gagagttcta tgaactttag atttgaagag gctgctgtat
    12061 atagggataa gataaagagc cttgaagaga tgatacagaa acaaaaaata gatgctactg
    12121 taagtgactt aaaccaagat gtggttgcaa tggcaagagc tcataatgaa gcttgtgtcc
    12181 aggtattttt tataagaaat ggtaagattg taggtaggga acattttata ttagaagggg
    12241 taatggatag tccaagagct tctatattaa gttcatttgt aaaacaattt tataatgaac
    12301 aagagtatat acctaaagaa ttaattatag aagatgagat agaagatagt tctatattag
    12361 aagaatggct gtcatctaaa aaggggcaaa aagtaacaat aagagttcct caaaagggag
    12421 agaagaaaag cttagttgaa atggttagga aaaatgctgt agaatatctt gaaaaatttt
    12481 ctgatatgaa taaaagaaaa tatgaaaaaa gtattggagc attggaagaa ttaaagcaaa
    12541 tattaaatct agaaaagttg ccaataagaa tagaggctta tgatatatca aatatacaag
    12601 gtgtagattc gattggttct atggtagtgt atacgaatgc taaaaaagat aagaaggaat
    12661 ataggagata taaaataaag acagttattg gacctaatga ttatgattct atggctgaaa
    12721 ttgtagatag aagattaaaa catggaaatt tacctgactt gatattgttg gatggtggaa
    12781 aaggacaagt tagtgcagta aaaaaagtac tagaactcaa tgatgtagat atacctcttt
    12841 ggggaatgta caaagatgat aagcatagaa ctaaaggatt gatatgcaaa gaaaaagaaa
    12901 tagaactaga taaaacaact aatttatata gatttattgc tagtatacaa gaagaagttc
    12961 ataattatgc aataacatat catagaagtc taagaaataa agctttgaca aaatctatct
    13021 tagatgatat acaaggaata ggtgaaaaaa gaaagaaatc attattaaat cactttaaag
    13081 atgtagatgc tataaaaaaa gctactatgg aagaactttt agaagtagat ggaatgaata
    13141 aaagcattgc agataatgtt tataattttt ttagaaagga agaaaattaa tggaaactag
    13201 caataaagta tcaataagag agataataaa agagttgaag ttaaaagtag tatacatgcc
    13261 tgatgatgta gattattatg tctactctca agatataaat agaccaggat tacagtttgc
    13321 aggatatttt gattattttg catatgaaag aatacaaata gttggaaaga cagaatataa
    13381 tttttttgga ttaatggata gtcatattcg ggaagaagta ctagatagat ttttctcata
    13441 tgaaatacca gcgttaataa tttctagaga cttggaaatc aagccagatg taattgaaaa
    13501 ggctaaaaaa tataaaagaa tacttttgag cacaaataga aataccacta gacttataaa
    13561 tagactgtca aactatttgg ataataagtt agcaccacac acaacaattc acggggtgct
    13621 agtagatatt tatggtatag gtgtactgat aaaaggtgaa agtagtatag gaaagtcgga
    13681 aacagcatta gaattgattc aaaggggtaa tagattagtt gcagatgatg ctgtagaaat
    13741 aagaaaatta gatgaaagta tgcttgtggg tcagtcacct gaactaataa gacattttct
    13801 tgagataaga ggaataggaa taatagatgt tagaagttta tatggagtag gggcaatcaa
    13861 aaattctaaa atgatagatt tagtagttca tttagaagct tgggatgaaa ctaagtatta
    13921 tgatagactg ggactggata aagaatatga agaaatactt ggtgttaatg tagagaaatt
    13981 agttgtacct gttaaacctg gaagaaatac tgctatgata ttagaagtag cagcaatgaa
    14041 ttttagacaa agaggaatgg gatacgatgc agctcaagaa tttactaaaa aattatcaaa
    14101 attaatcgat actaagtagt actgaataat agtgaactac acatcattta attaaatatt
    14161 gattaggatt atgtcctaca ccttataaat taaaatcaag ttgtatatat ataatgttta
    14221 ttaataagat atattatata aggataattt aaagtacagt gttagattat aataatacta
    14281 gattaatttt atatttgagt ttcattatat aacaagaaag ttggaatttt ttatgggaat
    14341 agaatatttt agtaaacttt tagaaagtag tactgaaaaa gaattgctct taagtaaaaa
    14401 gtcaaataac ataagtacag caagattaat atcttttttg atagtgatag cagggtttgc
    14461 tattggattt tacaataaaa atatagtagg tatttttgtt ggagttttat caataataat
    14521 atttatagca ctcttagtta ttcatagtaa agtaaaagaa gaagaaacat attttaaaag
    14581 taagagcgaa gtattaaata agtatattaa gagatttgga gatggttgga aggaatttaa
    14641 aatagatggt aaagaatact taaaagatga aaattcgcaa gcaaaagatt tagacttatt
    14701 tggaagagca tcattgtatc aatatatctg tgtagctaat acatcttatg ggaaaaattt
    14761 tttagccaaa tatttgtgga atgaaaatcc agatgagaat gtaatcttag aaagacagag
    14821 agctataaaa gaacttttat caaaagatga tttttctatt catattcaaa cactcagtaa
    14881 tataattggt aaagagcaaa aaaacaattc agatagtagt atagaatcgt ttatagaata
    14941 tggagaaaat aagaatgtgt atataccaaa atggatgcat atttttactt ggggtttacc
    15001 aactgcaaca atcttatctt ttatattttg tatgttagga tttttacctg ttttacctgt
    15061 ttttttactt tttataatcc agttagggtt tagtggtttt ggatatccaa gacttatgca
    15121 aactcttgct ccattatttt catttagtag aagtatacaa gtatatgaaa aaatgtttga
    15181 ggttttagaa aaagagacgt ttgagagtgc atatttgaaa gaattacaag ataaactttc
    15241 taagggaagt ggagtttcaa gaggtgttaa acaattaaat tcaataggaa atgctgtaaa
    15301 cctaaggtat aatcagatac tatatataat tgcctgtggt gttttgatgt ggaactatca
    15361 ttgtgcagaa gctttggagc gatggaaagg tatatatgga aatcaaatta gagattggtt
    15421 tgaatctatt ggagaatttg aagctctaat aagtttaaca gttgtttctc atgtaaagga
    15481 aaatacatgt tttccagtaa taaaatatga agatactcca agattgaaag ttgaagaggt
    15541 ataccaccca ttaatagcag aaaaaagtgt catagcaaat tcgatacaat taaattctca
    15601 gacttgtatt atcacaggtt caaatatgtc tggaaagaca acatttttaa gaagtatcgg
    15661 tgtaaactta gtgctggcat atgcaggtgc tccagtatgc gctaagagtt ttgatgcgac
    15721 ctgtatggct atatttactt ctatgagaat acaagatgat gtaagtcaag gaatatcaac
    15781 tttctatgct gagattctta gaataaaatc aatgattcaa tatagtttaa aagaactgcc
    15841 aatgttggtt ctagtagatg aaatttttaa gggaactaac tctgctgata gaattattgg
    15901 agcaagtgag gcagtaaaga aattatcaaa accctgggtt ataagtatgg tgacaacaca
    15961 tgactttgag ctatgtgacc tttcaggtag tggagatgtt gaagttgtaa actatcattt
    16021 ctcagagtac tatgtagacg ataaaattca ttttgactat aagataaaag atggaagatg
    16081 taaaactaca aatgcaaaac aacttatgag aatggcaggt attctttaat taatagatag
    16141 gttaattaaa gaataagtct atttagaaat ataatttaat ttgtgaaatt aataaggatt
    16201 gtcttaaatt acgtaaaagt attttaagac aatccttttt taagaataaa ttttatattt
    16261 tgttatgact acttagcttc ttcacaagca cattcttgtg gacactctcc atagacacaa
    16321 ctcttatcaa agtaatgagt atgaaggtat ttatgggcta catcactatt tggttctcca
    16381 aagaagtctt tataaatagc ttgtaaatca ggattctcat gagatttacg aatagcctta
    16441 tttctatcta tttcatataa cgcatttgca cgtctatcaa ctatttcagt attaccatgg
    16501 tgataaggtt gaccagcacc tccaacacat ccaccaggac atgccataac ttctatgata
    16561 tggtatttac attctccact tctgacctta tccataatct ttctagcgtt tttaagtgta
    16621 tttacaatac acacattaac tgtagttcca tcaacatcta tacttgcttc cctaattcct
    16681 ttaagacctc taacattagt gaagtttaca ttatctaaag tcttattagt tatcttctca
    16741 tatgaagtac gtagggcagc ttctaaaact ccaccacttg ctccaaatat agatgcagca
    16801 cctgtagatt ttcctagagg attatcaaaa tcttggtctt gaagattagg taaatcgatt
    16861 gcagcttctt ttatcatctt agctaactct ctagtagtta tagaaaggtc aacatctagt
    16921 attccagatt cacttaattc ttctcttgaa gcttcatatt ttttagctac acaaggcatt
    16981 acagatacaa cataaagttc ttcaggattg attcctaata ttttaggtgc atagtagttt
    17041 ttagctatag aaccaaacat accttgagga gacttacagc tagatgctaa gtttaacttg
    17101 tctggataat tgtgctctaa gaagtttacc catgcaggac aacaacttgt taatatagga
    17161 agattttcac ctttttggat tctttctata aattcagtag cttcttccat tatagtaaaa
    17221 tctgcaccaa agtttgtatc aaatacatgt tcaaatccaa gtgcttttaa agcagctacc
    17281 attttccctg tagagattga ccctggttct aatccaaact cttcaccaat agcaactctt
    17341 acagcaggag caacttgcac tactatagtc ttttcttttt tatttaaaac atcccaaagt
    17401 ttaggtacat tatctacttc tgttaatgct ccagttggac agacagatat acattgacca
    17461 cagaatgtac actctgtttc aaccatatca gagttaaaga atgtactaac taaagtatta
    17521 aatccacgat taactccaga taatgctcct actgtttgta tgtcattaca tacagtctca
    17581 catcttctac ataatataca cttagaatgg tctttaacta aagatttagt agaagtatct
    17641 ttacctgcaa aagatttaga accttgatat cttatttttc ttactcctaa atcagcagct
    17701 atagtctgta attcacaatc tccatttttt tcacagacaa aacaatcttg tggatggtct
    17761 gataacaata attcaacaat agttctacga gcatttaatg ctttagcact attagtctga
    17821 actttcatac cttcctttat aactgttcca caagctggaa ctaatcctct ctctgtctca
    17881 accatacaaa cacgacaaga agcacaagtg tcaagcttat ctatttcatt catatgtaaa
    17941 tgacaaaggt tgtgtatttt tatatttatt aatttcgcag catctaagat agaagttcca
    18001 ctaggtgctg aaacgtgttt gccattaata gttaaattaa ctaaactcat tgttattcct
    18061 cctccaaata agttatttct tgattatagc gttaaatgta caattatcta tacaagcacc
    18121 acattttaga cacttagaag tatctattgt atgttgttcc ttaacagaac ctgttataca
    18181 accagcagga cacactctag aacattttgt acaaccctta cagtcatctg ttataaagta
    18241 agaaagtaag ttctgacatt taccagcagg acatttctta tctacaacat gagctatata
    18301 ctcatctttg aaatattgaa gagtacttaa aacagggttt ggagcagctt taccaagacc
    18361 acataatgaa gcagtttgta tagtttcagc aaggttttct aggtcatcta aatcttgtag
    18421 agtaccatta ccttctgtaa ttttagttaa tatttctaat aatcttgtag tacctatacg
    18481 acaaggtgta catttaccac aagattcttc aacagtaaac tcaaggaaga acttggctat
    18541 atctaccata cagtcagatt catcaagaac aagcatacca ccagagccca tcattgaacc
    18601 gatagaactt aatgaaccaa agtctatagg agtgtctaaa tgttcagttg gtatacatcc
    18661 gccagatggt ccacctgttt gaacagcctt aaatttctta ccttcaggga ttcccccacc
    18721 aatttcaaat acaatatctc ttaaagtagt acccattgga acttcaacaa gtcctacatt
    18781 ttctactttt ccaccaagag caaagacttt agtaccacta gaatcttcag tacctaagtt
    18841 ggcaaaccaa tctccaccct taagtataat tgcaggtatg ttagcaaaag tctcaacgtt
    18901 atttaagcaa gtaggagatt tccatagacc actcttagaa gaactataag ttttcattct
    18961 aggttcacca cgtctacctt caattgaatg cattagcgct gtaccttcac cacaaacgaa
    19021 agcacctgca ccatatttta attctaattt aaagttaaat ccagtgccta agatattttc
    19081 gccaagtaaa ccgtattttt cagcttgagc tatggctact tttaatcttt ctatagattt
    19141 tggatattct gctcttatgt atatgtatcc tgtatcagaa cctatagcgt atccacatat
    19201 tgccatagct tctagtacac tatgagggtc accttcaagt acagatctat ccataaatgc
    19261 acctgggtca ccttcatcag cattacatac aacaaacttt ttatctgttg gaccagctgg
    19321 attttttgaa gcagcttccc atttagagcc agtagggaaa cctgcaccac ctctaccacg
    19381 aaggccagaa gtttttacct ctgcaataac ttcttgagga gtaagagaag ttaaacattt
    19441 gcctagagct aaataaccat cattggcaat atattcttct atactatctg ggtctataag
    19501 accacaattt tttaaagcta tacgtaactg ttttttgtag aaagacattt cttcttgttc
    19561 ttcaactttt ttacttaaag attcttcctc atataataat tcctcgacaa ctgtatttct
    19621 aattaagtgg ctttgaacta tcttttcagc atcagatggc tcaactttta cgtagaaaac
    19681 gttatcagga taaactttta caataggacc ttgagcacag aaaccaaaac aacctgtcaa
    19741 acgaacacta actttatctt ggattcctga tttctttatt tcattttcta attcactaac
    19801 tatttctaga ctgtttgaag atgtacatcc agtatcacaa caaacaagta attctcttct
    19861 taatacattt tccttagaat aattttttct taagctaaga ttaggtttca gctcatcagc
    19921 taaaactttt aattcatcaa atgaatttac tttacgcatt atttattcac ccctcttaac
    19981 aattcaattc taattctcta taagtatcta atatttcgct tacttggtca ggtttcactt
    20041 taccataaac tttaccattt acagtaacaa ctggagctaa accacatgcc cccaaacatc
    20101 ttaaaccttc taaagaaaat ttaaaatcac tagtagtttc tccaactttt atgccaagct
    20161 gtttttcaaa agcacttaag attttgtcag caccttttac aaaacataca gttcctagac
    20221 agacactgat tttatactta cctacaggtt ctgtagtaaa ataagaatag aatgtaacaa
    20281 ccccatatac ctttgcagga gctactccaa gttttctagc aacatgaagt tgaacttctt
    20341 ttggaagata tccaaatata ccttgggctt catgtagaac ttgtattaac gcaccttcct
    20401 ttgttgcaag agagtcaata aatatatcta gttcgtcaaa taattgcttg ttgtgtgaaa
    20461 taaaatcgca cataacaaat cccccttttc tttttaaagt tattattttt cataaaataa
    20521 ttaaccatga gttgacccca acattatgtg ggtgaacaaa accaaaacaa taaagaaaga
    20581 ataatataca acaatttctt tatttataaa aacaatgcaa aaaagttacc tattaaaaat
    20641 aaaaactata ctagtctatt ataatataaa ataattcaga atagttaaaa aaaataaaaa
    20701 aaatgagcta aattcaataa aaaaaaaata atacatataa aaaactttaa aaaataaacc
    20761 aaaatgtaaa atttttataa aataaaagat ttataaaact gctgataata gtcttgaaat
    20821 tgattccttg atttttatat aagtagacct agaattatat atatctaatg ttatttcctt
    20881 agaatttaaa atatcatttt caaatataat tctttgttcc aatgcttttt ttgaagagta
    20941 cataaatgca ttaacttcga aattaagctc aaaacttctg atatccatat tagctgttcc
    21001 tatagagcat atagagtcat ctataactat tgtcttagca tgtaaaaatg cattttgacc
    21061 ataagtatag attttagctc caaactttaa aagttctcca gcatatgaat atgatgccca
    21121 gtatacaaag ggatggtcag gtttagaagg aatcataact ctcacatcaa caccagataa
    21181 acaagctatt tttaaagtat ctataaaagt tgagtcaagt attaaataag gactctgtat
    21241 gtatatatac tttctagctt tctgaatcat tttaaggtaa ccatatttaa tctcatcaag
    21301 ttctgatata tctggaccac ttgatactat ctgaatccca atattttcag aaggatgatt
    21361 agaatttgta tcttcttcta taaagtactt ttctaaatct aagtcttctt tagttgtata
    21421 tctccaatca aggatgaatc tcatatttag gtcaattacg caatcaccag taagcttagt
    21481 atgtgtatct ctccatagac caaattttgg atttctacct aaatattcat caccaacatt
    21541 atatccacct acaaatccta ccttaccatc aatgacaaca atttttcggt gatttctata
    21601 gtttaagtta aagtttacaa ttttcaagaa tgatgggaag aagcttccaa ctttaacacc
    21661 actttcttta agtctggaaa gtgtactatt ttttagtgtt ctaccaccaa ctgcatcaaa
    21721 taaaagtctt acttcaacac cttctttagc tttgtctata agaatgtcta ttatttcagt
    21781 tccgattttg tcatctttga aaatgtaaaa ttgtatgttt atatattttt tagctttttt
    21841 aagttcttca agaagactgt taaaaaattg actagattca gcataaatcc acacatcgtt
    21901 attattggta taatgagcat tatttgaatt agccaatgca taaatcatat ccttatgttg
    21961 atgtatatct gaatcaattt cagatgtaga ttggagttta acctgagtat ctagtatatt
    22021 acttttgatt atcttatcat ccttttcttt caatctaaac atattgtttt ttgaaatatt
    22081 tctgccaaaa gctatataaa gtatgaatcc aagagcagga atcaatacta atataagaag
    22141 ccatgttaaa gttgtattta tgtttctttt ttctctaaag ataaggttta gtataaccat
    22201 gaaattaatt atatatattg tagtaactgc tatttcatat attgacattt cgaaaaatga
    22261 tatatcaaac ataaaattcc ctccttcaaa tatttattaa aataagtaat tatatataat
    22321 aacacaaaca tatatctatt atatataata cctcaataat taaaaagtaa
//
LOCUS       DACULQ010000050        21471 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.050, whole genome shotgun sequence.
ACCESSION   DACULQ010000050 DACULQ010000000
VERSION     DACULQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.050"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..288
                     /locus_tag="JA154_07725"
     CDS             <1..288
                     /locus_tag="JA154_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAT7995753.1"
                     /translation="KILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRNDYWIF
                     NPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(430..690)
                     /locus_tag="JA154_07730"
     CDS             complement(430..690)
                     /locus_tag="JA154_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HAT7995754.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            907..2190
                     /locus_tag="JA154_07735"
     CDS             907..2190
                     /locus_tag="JA154_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAT7995755.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            2198..2875
                     /locus_tag="JA154_07740"
     CDS             2198..2875
                     /locus_tag="JA154_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HAT7995756.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDKAIKSLE
                     NAKNLKK"
     gene            2961..3404
                     /locus_tag="JA154_07745"
     CDS             2961..3404
                     /locus_tag="JA154_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAT7995757.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            3709..4353
                     /locus_tag="JA154_07750"
     CDS             3709..4353
                     /locus_tag="JA154_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAT7995758.1"
                     /translation="MSNYRTLHEFGMDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(4439..4816)
                     /locus_tag="JA154_07755"
     CDS             complement(4439..4816)
                     /locus_tag="JA154_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAT7995759.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(4816..5202)
                     /locus_tag="JA154_07760"
     CDS             complement(4816..5202)
                     /locus_tag="JA154_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAT7995760.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            5381..6136
                     /locus_tag="JA154_07765"
     CDS             5381..6136
                     /locus_tag="JA154_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthetase"
                     /protein_id="HAT7995761.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            6219..6938
                     /locus_tag="JA154_07770"
     CDS             6219..6938
                     /locus_tag="JA154_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAT7995762.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(7105..8109)
                     /locus_tag="JA154_07775"
     CDS             complement(7105..8109)
                     /locus_tag="JA154_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAT7995763.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            8375..9271
                     /locus_tag="JA154_07780"
     CDS             8375..9271
                     /locus_tag="JA154_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HAT7995764.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREMNLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            9409..10671
                     /locus_tag="JA154_07785"
     CDS             9409..10671
                     /locus_tag="JA154_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995765.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            10747..11940
                     /locus_tag="JA154_07790"
     CDS             10747..11940
                     /locus_tag="JA154_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HAT7995766.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSIKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            12581..13363
                     /locus_tag="JA154_07795"
     CDS             12581..13363
                     /locus_tag="JA154_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAT7995767.1"
                     /translation="MENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQVIDVV
                     SENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGVFDKIEALEKTVIA
                     AVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTGIAKE
                     LIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVINKGI
                     DMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            13458..14798
                     /locus_tag="JA154_07800"
     CDS             13458..14798
                     /locus_tag="JA154_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HAT7995768.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14929..16167
                     /locus_tag="JA154_07805"
     CDS             14929..16167
                     /locus_tag="JA154_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HAT7995769.1"
                     /translation="MKVLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFG
                     DPGRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSS
                     YRTGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDI
                     AEKDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSL
                     LASTQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIK
                     EEKFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQ
                     AIANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSD
                     IEALIESGIVTMNKEACEIC"
     gene            16207..17349
                     /locus_tag="JA154_07810"
     CDS             16207..17349
                     /locus_tag="JA154_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT7995770.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            17360..18154
                     /locus_tag="JA154_07815"
     CDS             17360..18154
                     /locus_tag="JA154_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HAT7995771.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            18165..19187
                     /locus_tag="JA154_07820"
     CDS             18165..19187
                     /locus_tag="JA154_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAT7995772.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            19286..20662
                     /locus_tag="JA154_07825"
     CDS             19286..20662
                     /locus_tag="JA154_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAT7995773.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNRSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20994..21338)
                     /locus_tag="JA154_07830"
     CDS             complement(20994..21338)
                     /locus_tag="JA154_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995774.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT         8052 a         2525 c         4120 g         6774 t
ORIGIN      
        1 aaaatcttaa aaactacatc aataacactt ataattatgc cattattatt aacagttcca
       61 attttattaa attttgaaaa gtcatttatt atttttcata aattattatt tagaaatgat
      121 tattggatat ttaatcccga tttagaccca gttataaata tattgccaga ggaatttttt
      181 tttcactctg gaatgatgat tttaatatta attttgttag taagtatagt actgtttgta
      241 atgtataaat tatgtaaatc tttatcaata aaaatatttc aatgttaaat agagggatat
      301 ttttatttcc aaaataagat aataaaaaag gattagatta aaagttatac aataaaaata
      361 gctgtgcaat aatatttaga ctatgaaagt agttaaaaaa tatttatgca cagctattta
      421 taatagaatt tactttttac tagtgatttt aactaaatct tgaaatttac tcataatgta
      481 attgtaatta tcttttttat gaggtaatat acaactactg caatctttga tacctttatc
      541 tgtatactta aagtttccac cacagttttc tcctaaagca tacaaaggac aatagcaaaa
      601 tagacagtta aactcttctg gtttattagt tttatgacaa gggaaaaatt cgcaatcttt
      661 gtgattaaaa aacttataat tttcgcccat attttcctcc taattaaata aaatttaaat
      721 tttttatact atataagatt ttggtgataa tataattata ctaaataatt ttatgaatag
      781 tcgataatat agtattatta atcatagtat taatggtata attagtcaac agaaaaataa
      841 tgtaaaaata atgtaagtgt attttgtaat tagaatatgt tttataacaa aaaatgaggt
      901 gattaagtga aagaaattca agaaaaagca cttcttgtag gtctgaattt gactacaata
      961 gtgaaaaaaa atgatgatat agacactaat gaatctatgg aagaattaaa agaactaaca
     1021 aaagctgctg gggcagaagt tgtaggtagc ttaattcaaa ataaacactc tgtagatgca
     1081 gcttattata taggaaaagg taaagtagaa gaaataagag cctatagtga ctctttagat
     1141 gctactttag taatttttaa tgatgaatta tctggtgctc aaatcagaaa tatagagaat
     1201 gttgttggaa gaaaagtaat agatagaact actttaattt tagatatatt tgctcaaagg
     1261 gcacttagca aagaaggtaa attacaagtt gaattagcac aattaaagta tagattgcca
     1321 cgactttatg gaatgggtgg tgagatgagt agaacaggtg cagggattgg tactagaggt
     1381 cctggagaac aaaaactaga aatagataag agacatatac ttaataaagc agcagatata
     1441 agacgtgaac ttaaagaagt aaaaaagaat agagagacac agagagttaa gcgtttaaag
     1501 tcaaatatac cgattgtagc acttgttggg tatacaaatg caggaaaatc taccctgttg
     1561 aacgaattaa taaagacaca caaggattat gaacaagaaa aagaagtttt tgtaaaagat
     1621 atgctttttg caactttaga tgtaaccttg agaaaggctc ttttgccgaa taaaaaagaa
     1681 tttttagtag tagatactgt tggatttgta agtaaacttc cccatgattt agtagaagcg
     1741 tttaaggcaa cattggaaga agtacagtat gcagatttaa tcttacatgt aattgatgca
     1801 acaaatacta gttatgaatt gcaaaaaagt acgactgaag gtgtattaaa agaacttggt
     1861 gtaaatgata aaaaacatat acttgtatat aataaagtgg acaagctaga attggatata
     1921 tatcctaaaa gtcaagaaga tattgtgtat atttctgcaa aacaaggcat aaatatggat
     1981 aagttattaa atatgataga aattgcttta atggaaaata cttattcagt aagtttgatg
     2041 ttaccttatg agagaggtga tatatttagt agaataaaag ataaatataa tgtggagaat
     2101 tttgagtatg gggaaagtgg tataacgcta gatgtagatt tagatgaaga agattttaat
     2161 atctatagag agtacatact agaaaaatag gttgaatatg gatataaact gggaaaatat
     2221 agataatctt gaagattatt ttataaccta tcttctttat aaagaatcta aaacagtatc
     2281 tcaaattagt aaaattagaa atatttcaag tactgaagca agtgagcaac ttatacaagc
     2341 aaagttaaaa ataaaggaaa tgcaaaaaga tgattttgag gcatctaagg atattttgga
     2401 taaatttcta gaactagata aaattaagag acttgatttt atggatagct tagatgacga
     2461 aaaaatggta tactttaaga gaaaggtttt caaaagaatc cttgtagaaa aaaatgctga
     2521 agatttaatt gttttaattt gggcgacagg tgagctaaaa gatgataggt ttttgaaatt
     2581 gcttcatcag ctcacaaacc acagacactc agatattaga agaataactt attcagcaat
     2641 cagaaagatt gaatcaccaa gtagtagaga agtactgcaa aaaggtctct acgacaaaaa
     2701 tgcacagact aggcagtact gtgcaaaagc tctttcaaaa ttgggagatg aaaacagttt
     2761 aaagatttta cagcagttga aagacaagaa taagaatttt gaaaaagaat atgtgcttag
     2821 agcttatgac aaagccatta agtcattgga aaatgcaaaa aatttgaaaa agtaattttt
     2881 tattaaagca atttgtttac ttaatgatgt atttctaaag tttaacaagg taataaataa
     2941 attaatggga ggggtttcaa atgatagtaa gacgttgtgc aggtggagta gtgttttatg
     3001 ctaataaagt acttattgta aaaaatgata ggggtgaatg gacacttcca aaaggaaaga
     3061 tactaggagg gggacttcca tatgagagtg ctgttcaaag agttaaagta gaaactggca
     3121 tagatgctaa aatgatagat gtggctggcg atactatgta tgaatttttc tcaagaagta
     3181 gacaacaaga agtttgtaat gcaataatgt ggtatgttat ggaagcttgt aacactgatt
     3241 gtgtacttgc tcctgaattt caagaaggtg gattttataa agtaaaggat gctttagaaa
     3301 tgttatcaca tcataaagag caagctttag tagaagtatc ttataaaaag tttaaggaat
     3361 taaagaaatt agtttcagag ggcgatgatg ttccagcaaa ttaattgtga atttagagga
     3421 ttctgaggaa gggatagccc cttccttttt taattgaagc agttttaagc gtaaattata
     3481 tagtctcttt aaactatgat ttatcaattt agagatttag tagtttatat ttaaaatcag
     3541 taagatatat ttaaaataaa aattaaaaag tatacaattt tattatataa ttgtcttatg
     3601 atgttgatta aagaattatt aaaagtccat ttatttttaa atagaataat tttataaata
     3661 tatatttgat gtgtatatta gaaaatgatt aaaataggag gaaatacaat gagcaattac
     3721 aggacattac atgaatttgg aatggatgaa ataacgattg aaaagtctgt ttttattgga
     3781 tatgctaagc ctataaagag tgaagaagaa gcattagagt ttataaatga aataaagaaa
     3841 aagcataaag atgcaactca caatgtgtgg gcttatactg taggtaaaaa tatgaatata
     3901 caaagatata gtgatgatgg agaaccacaa ggaacagctg ggatacctac attagaggtt
     3961 ataaaaaaag aagatttgag agatgtagcg gttgttgtta ctaggtattt tggaggaaca
     4021 aaattaggtg caggtggtct agttagagct tatacaaaag gtgctaaact gggacttgaa
     4081 gcaggtaaga taatttataa ggtaatgtat caagaagtta aggttaagat agattatact
     4141 caacttggaa aagtgcaaaa tgagttaatg aatttggggt attttataaa agatactgta
     4201 tatgaggata atgttgaaat agtagtatat tcaagattag aagatgtaga gaaattatca
     4261 gaaaaaatga ttgatataac aagtggaaca ggaaaaatag ttttaggaga ggaattttat
     4321 ttgtctgaac aaaatggaga aattttaata taatctaaaa ggttattaga atagttaata
     4381 cctcaaatag ccaattagta tttttaagtg aaaagccatt aaattgattt aatctaaatc
     4441 aatttaatgg ctttttatcg gattttgcac agaatccttt ttttacttca aataaaccga
     4501 ctaaaagtaa aaatatccca aatatttttt ttaatatatc tgaagacaga taattagcta
     4561 atagtgaacc aaatacagct cctaaaaccc caaacacagc tataggtata accaatttaa
     4621 aaattacatt tttatttttt atatgtatag caagtgctat aagtgtcata gggatagatg
     4681 aaagtaaatt tacgctttgt gctattttag tgtctatgcc agcaagtaaa acgagtgcag
     4741 gtataaggat tgttcctcca cccattccca ttcctccaat aattcctgca aaaaatccaa
     4801 ttagagcaaa taacattaaa acaccatcct taatgctgca attatcataa ttattccaaa
     4861 agaaattcta agaaactttc cagtaacctt atttagtaat ttagctccta taataccacc
     4921 aagaatactt ccaactgcaa ctttaattgt taagttaaca tcataagtac ctttagatac
     4981 atataaaaca gaacttgctg tcgttaagaa tattattata gaaagagcag ttgcatgaga
     5041 tttatgttct tctaccttaa cgatgtcatt taatattgga actaaaagag tacctcctcc
     5101 agaaccaaaa actccattta taaatcctgt aaaaactcca attatcgtat tttttaagtt
     5161 aaaatcaaag tattttttct ttaaatcatc agtttttttc ataaaaatca ccttctgaaa
     5221 tagttttacc aaaataggca aattatattt atataaaata agattaaata taatctttca
     5281 tgtttgttta attttgcata aaaaaaaaca tattaaaaca acatattaat atatggtata
     5341 atatattaaa acaaagtagc aaatttgaga ggagataaca atgtcaaata taaaaataca
     5401 aatagacaaa actgtagaat ggcttataaa taaggttaat gaggcaaatg caaaaggatt
     5461 aattgttggt gtatctggag gaattgattc ggcagtagtt gctaatttaa ttaaaaaggc
     5521 atttccagaa aactctatgg gagttataat gagcataaaa agtaatcctc aagatagaga
     5581 agatgcactt aaggttatag agggatgtga tatagaatac cttgatttag accttataga
     5641 accacaaagt gctatattag acatggtagt aggaaattta aaagataaac atttatatag
     5701 agaagaatat ttaaagatga cagatgcaaa cttaagagca agagtaagaa tgagtacaat
     5761 ttatacaatt gctaacaatc taggatatct ggttgttggt acagacaatg ctgcagaaat
     5821 acacacagga tattttacta aatttggtga tggtggagtt gatattttac ctattgcaaa
     5881 tttaactaaa ggagaggtgt atgaatgggc aaaagagctt ggtgttcatg aagatttaat
     5941 aaacaaagct ccttcagcag gtctttggga aggtcaaaca gatgaagatg aaatgggaac
     6001 tacttacaat atgatagatg ctgtgttaga aggtagattg gatgaagttc ctaaaagaga
     6061 ccaagagata attgaaagac ttcatagatt aagtgaacac aagagaaaaa cgcctgcaca
     6121 acctcctaag ttctaattta ctatttataa gatttgtgat aaaatttaca agtgatgaaa
     6181 attagaataa ttaattgtac gtattaagga gtggaaatat gggacgtata ggtaatataa
     6241 ttaatagaaa aggtaaacaa gatgctcaaa gagcaaagat atttactaaa catgctagag
     6301 caatagcagt tgcagctaag gaaggtggag cagacccaga gtacaatgca gcgttaaaaa
     6361 cagctataga aaaagctaag gcagataata tgccaaatga taatatagat agagctatcg
     6421 ctaaaggtgc tggagcaggt gctggagaag actatgaaac tatagtttat gaaggatatg
     6481 gacctggtgg agttgctgtt atagtagaaa ctttaactga caacaaaaac agaactgctg
     6541 gtaacgtaag atactatttt gacaaaaacg gaggaaactt aggaactagt ggatgtgtgt
     6601 cttttatgtt tgataaaaaa ggacaaatat tagttggttt aggagatgga gtttcagaag
     6661 aagaattgat ggatgttgca ttagaagcag gagctgaaga tttcataaca gaagaagatg
     6721 gttatgaaat aataactaca ccagaagatt tttctagtgt tcgtgatgag ttaaaagcta
     6781 aaggatatga gtttatatct gctgatgtga agatgatacc tcaaactaca actgtattga
     6841 ctgaagaatc tcatttaaaa atgatgaata aattagttga tatgcttgaa gaagatgatg
     6901 atgttcaaga tatatatcat aactgggaag tagaataaat agtataagca tattgaaatt
     6961 tataagtatt gaatcaattt taacatgtga taaattttat agaataagaa acactaaaat
     7021 agacatacgc acctctaacc tatatttgca taataatatg taagcatagg taaggggtgt
     7081 ttttatattt ataggttaag aaatttaaag taaattttga gctggactcg attgtctata
     7141 aataattgat gggtatataa cttgttgctg aataccattt atacgcccag ttactgtatc
     7201 aataatcatc ctaagactac gttctgctgt ctcttccatt tttaaatcta taactgtcaa
     7261 aggaggaata cttcttgcca ttagtcgtga aggtccatta cttgctgcaa atatttgaat
     7321 atcctctcca attacaattc ccgattcatg aaatgcattc atcataccaa gagctaccac
     7381 atcatgatta caaaaaatta ctttttttag tgaatttttt gcaagcattt tttgagcaat
     7441 atcatatcca ctatctattt tattagaaca ataatatata gaatctgata gatgtattcc
     7501 atgttcttta catgttttta aaaaagattt accccttaaa gtcatacatg taaaggaagt
     7561 tgggttgaat acaacagcaa tatcattttt accattttga atagcatgta aagctgctat
     7621 acgaccagtt tcctcattat caatagatac acttgaaaaa ccaggtattt cacgatttac
     7681 aacaactaca ggaattttag gtttattttt tactagatat tctatatcat cagcattgat
     7741 acctgttaaa acaattccat cataataatt atttgcaaaa aagtattcat catcacatag
     7801 atgaccaact ttgtaaggtc gtatgatgat atttatgttt aaatgttcta aagaaattat
     7861 gtggtttatt gcttgaagta tattggttga tgccaactca aatccctcat cctgccaata
     7921 aaaagctatt ttaatattat tatgtttaat aggagtagag cgtttatatc caagcttatc
     7981 agctgaattt aatataagct cttgtgtatg tagcggaatc ctgcgttcat ttcctttacc
     8041 acttaagacg tacgagacag ttgctgaaga aagaccacag tcttttgcaa tatctttgat
     8101 tgttgccatg ataaaatcct ccttaaatag ttgcttaaat ttgcttatac caataatctt
     8161 aacatatatt caactaaaga acaatattct acaaggtgca tattttacag aattagaata
     8221 aataaattta aaaaaacttc ttacttatgt tgacataatt tagtttatag gattataata
     8281 tgcttaaata agcttattat taacttaaat taagcaaagt taagcaggaa catgcaatgc
     8341 tagcttgcaa tgtgactgta aagggggaaa aggtatgagt aaggtaaata tatttgaagt
     8401 tggtcctaga gatggatttc aaaacctgaa agagttttta gaaactgata aaaagcttaa
     8461 gattattgat ggtcttattg cttcagggat taaacaactt gaaattagtt catttgtaag
     8521 cccaaaagca atacctcaga tgaaagatgc aaaagaagta gcaacttatt gtgtagaaaa
     8581 atatcctgat gtgaaacttt atgcattagt tccaaattta tacggtgcta aagttgcatg
     8641 ggagtgtggt ataagagata tatcctatgt tatatctttg agtgaaactc ataataaaaa
     8701 aaatataaat cgtacccatg aagagtctat tgatgagctt aaaaaaataa tgaatactta
     8761 tccagacatg aatatatgta ttggaatggc tactgtattt ggatgtcctt ttgaaggttt
     8821 accaaaaatt gaagatgcca taagatttgc taaacgcctt tgggaaaacg gtatcaggga
     8881 gatgaatcta gcagatacaa tagggattgc tgaccctaag caagtaagag aaactattag
     8941 tgcattagtt gaagctttac cagagtgcag atggcaggta catattcatg atactagaaa
     9001 tatgggaatg gttaatactc ttgtggctat tgaatctgga attactactg ttcagtctac
     9061 acttggagga ttaggaggat gcccatttgc tcctggagca tctggcaata ctgcaactga
     9121 agatttagta tatatgcttg aacgtatggg gtatgataca ggagttgatt ttgttaaact
     9181 tcttgaatta tcaaagtatc agaagagttt tgtaaatgga gtatatagtg gtcatcatgt
     9241 aaatataaag tctgatagtt gtaattgtta gttctattga ttaatcgatt aaaaaaatat
     9301 gttgattaaa aaaatatgct gattggtaaa atagatttat tgataaaaaa tataaattat
     9361 aaaatacatt atctaataaa acaaataaaa aaattgaggg ggattagtat ggatattata
     9421 gctttacttt tatttttagg agttattatt attgcatttg tacgtaagaa taatgcaggt
     9481 attttggctc tagctattgg tgtcatagct gttagaattt ttggaatgga tgataaagaa
     9541 ctttatggag ctataagcaa ctcaatgttt actacacttg ttggtataac actactcttt
     9601 gcaatagtga actcaacagg agcattaaag cttgttgcag aaaaaattgt agctgcttct
     9661 ggtaaacgtg tttggcttat acctatagca gtttttatag caggatttgt tgttgctggt
     9721 gttggacctg gggcaattcc agcacttgca attatcccag caatggctgt tcctgtagcc
     9781 ataacggttg gttataatcc tgtaatgctt gcacttatag gtgaatgtgg attaatggca
     9841 ggtcgtatga ctcctattac tccagaaggt caaattataa caaccgttgc agaaggggtt
     9901 ggaattgaaa atgttatgcc tactatctta gcctgtcaaa ctctttctac aattgtattt
     9961 tcaatagtat tgtttataat tttcaaagga tataaactta aaaaacctat aaatgttata
    10021 gatagtaagc aaatagaaaa attcaatata aagcagataa tttctctaat aagcatagta
    10081 gttatgattg cttttatcat aatttttgat atgaatgttg ggcttgcagc ttttgcagtt
    10141 tcagctgtac ttattttttt caatattgct gatgaaggag aatgtatcaa atcaattcca
    10201 tggagcacga tagtaatggt tcttggagta ggtgcaatga tgacaatcgt agatgcagca
    10261 ggaggtattg acttgatgag taatgcatta tcatcactga tgaatagtaa aacagctact
    10321 ccaattatga gtatttcagc aggacttatg tctatggtta gttctgcttt agcagtagtt
    10381 tatccaacta tgatgcctat gtgtgttgat atagctaagg cagttggagg agtagaccca
    10441 ctagcactta ttgctgcagt tggtgttgga ggttctcttg ctggtgtaag cccactttct
    10501 actggtgggg cattgatttt agcagctatg ggttcaagta agaaagactt taataaggaa
    10561 gaacaaaata aggtttttgt acagcttttc attatgagtg cagttggctt attggttatt
    10621 gcagtagtat caatattatc atttaatgca ataacacatt taatgaacta attagaataa
    10681 ctgaaaataa atcattaaat ttaactttta atattaatca atataatata atttggaggg
    10741 gaagttatga aaaaaggagc attaagcaat ataaaggtac ttgaccttac aagagttctt
    10801 gcaggtccat attgtacaat gatgttggcg gattttggag ctgatgttat aaaaatagag
    10861 atgccaagaa aaggtgatga tacaagaggt tttggacctt ttgtaaatgg agagagttta
    10921 tactatgcaa atataaatag aaataaaaaa ggtatcactc taaaccttaa atcagaagaa
    10981 ggaaaaaata tatttttaga aatggtaaaa catgctgatg ttgtagtaga aaattacaga
    11041 ccaggtgtaa tggaaaagtt aggtctgggt tatgatgagt tggctaaagt taacaatcaa
    11101 ataatttatg gagctgtatc aggatttgga tgttatggac catattcaga acgtccaggt
    11161 tatgatatta ttgcacaggc aatgggtggt cttatgagta taacagggca agaaggagga
    11221 gaaccaacga gagttggtaa tgctatgggt gatgtactag gtggaatgaa tttaactatt
    11281 ggtatactca cagcactcaa cgctagaaaa ataataggaa aaggacaaag agtagatgtt
    11341 tccctcgttg attctgttgt atcgtctctc gaaactggta tacagagata ctttgattca
    11401 ggacaacctg aacttatggg taatagatat gcttcagcct atccttatga ctcttttaag
    11461 gcaagtgatg gaagttttgt aataggctgt ggtaatgaca aactttttga aaagttatgt
    11521 aaaaaagttc tttttagaga agacctttta atagatgaga gatttaaaac aaatcttaat
    11581 cgttgtgaac attacaaggc tcttaaacca gaaatagaaa aatggtctat taagcataca
    11641 ataaaagaag cagttgactt aatatcagag gctggagttc ctgctgctcc tatctataat
    11701 cttgattgtg ttacaacaga tgaacatata gcaaaagcaa gagaaatgat tgtagatatt
    11761 agtcatccta ttataggaaa tatgaaagtc aatggaaatc cagttaagtt gatgacaaca
    11821 aaagctaaaa ttgatatgcc agcaccatct ctaggggaaa gtaacgagga gatttataat
    11881 ggttggcttg gttttgatag tgataaaata aaagatttaa aagaaaatgg agttatttga
    11941 ttaaaaaata gctaaacaca cttgtgactt tagtcagggg aggttcagtt agaataatat
    12001 caaaaaatat taaatcaata ctgtaaatca aatatagaat tgtcagaagc atttaagatg
    12061 ggaaaagcta cttagacatt gagtctttgt ggcttttcct attttcatta agaattttat
    12121 attatcagaa tagatatgta ctaaactaaa gtattaacaa tttaaattaa tagaaaataa
    12181 actacctaat tatgttgaat aaagactact ttagtgaaaa taaacttata gagatgctat
    12241 ttaaattatt ttaatggaaa tatcaaaaat gagactgcat tctcaaaaat gagactgcgt
    12301 tttttaagta ttttctcgaa aatgagacaa aaatattgtt ttatgggttt tattgaagaa
    12361 aaattcttct gtgaattaca atttaatcaa ttttaaagtt gaaaattatt aaaaatttag
    12421 tgaaatttat gtatatttcg tttattttat taaaaataac aattttttac taaaaataaa
    12481 gaacttcaat ggtgattggc atagtacttg ctattttata ttaacaagaa atgtagaaaa
    12541 aataacaatg tataatgggg ggaatatgat ggaatctgta atggaaaatt tgaacaatct
    12601 taaggtggag gttaaggaca aagtctgtgt tattactata aatagaccta aagctttaaa
    12661 tgctcttaat agtgacactc ttagggaact atcacaagtt attgatgtag tatcagaaaa
    12721 tgaggcaata ttaggagtta ttattactgg agaaggtaaa gtttttgtag ccggtgctga
    12781 tatacgccag atgcaaaact ataaaagtga ggaaggcaga aaatatgcag gttatgctca
    12841 gggagttttt gataaaattg aagctcttga aaaaacagta atagcagcag ttaatggata
    12901 tgcattaggt ggtggatgtg aacttgctat gagttgtgat attagaattg catcagaaaa
    12961 ggctatattt ggacaacctg aagttaattt aggagttatt ccatgttttg gtggaactca
    13021 aagattatca agacttgttg gaactggaat agctaaagag ttaattttta cagggagaca
    13081 agtaaatgca gaagaagcta aatctattgg gcttattaat aaagtggttc caagtgattt
    13141 acttttagaa gagtctatga agatgatgaa ccaaatagtt gaaaaagcgc caatagcaat
    13201 aagatatgca aaagttgtta ttaacaaagg gatagatatg gatttaaaga atgctcttga
    13261 actagagaaa gatattgcag gtttaacttt tgctacaaga gataagcaag aaggaatgaa
    13321 tgcatttata gaaaagagaa agccagtttt tgaaaataag tagctattat aactaaaatt
    13381 ggaacaatgt agaatatatt caaaatctaa atttcgtgaa aaaatacata tagtttaatt
    13441 ttaaaaggag ttaatttatg aatacgggag taattataag tttagtaggg attatcatag
    13501 ctctatctct tctagttgta atggttatga agggaataaa tatttttatt atagcaattg
    13561 tatgttcatc attagttgct ataacaggaa gcctaaatgt gtatgatgca cttaaagtgg
    13621 attatatgac agggtttgtt ggatttcttc aggcaaattt ctttattttc ctaacaggga
    13681 cattaatggg aaaaataatg gagattactg gtggagcaaa atctattgct aagatgattg
    13741 taagatggat tgggaaggat aaagcactac tttctattcc aatagcttgt ggaatattag
    13801 cctatggtgg agtaagtgtt ttcgttgtaa gttttgcagt gtttccgata gctttagaaa
    13861 tttttaaaga agctgatttg ccacgaagat ttataccagc agctttgact tttggatgct
    13921 caacttttgc tatggttgca ccaggggcac cacaaattca gaatattgtt ccagcatcaa
    13981 cattagggac agatattatg gctggagcag taaatggttt tatatcatgt gcagtaatgt
    14041 ttatagttgg aagtacaatt ttgtacaaaa tggtttcaaa agaaaaagcc aatggtggtc
    14101 attttatagc acatgaatca gatatatttg atgaagcaga agcaactaat tctaagagag
    14161 aaaatggtcc caatggtgta attgccttaa ttccattgat agtgtcaatt ttaataatta
    14221 atgtaaaagt taatggaaaa gcgatagtac caattgaggt gggagtattt attggagctt
    14281 tattagtata tttattgtta aataaatatc aagataatac taaaatagtt ggacatgttg
    14341 gtgatgcatg taaaactaca gtagtagcaa tatgtaatac ttgtgctgta gttgcatttg
    14401 gttcagtagt aaaatcagca gttggttttg acttcatagt taatgctatg actaatattc
    14461 ctggtcctcc tattgcaggg gctgcagttg gaactacagt tattgcaggt atttgtgggt
    14521 cagcttcagg tggactagga attgcagctc cacttcttgg accagtattt ttagccaagg
    14581 gtgtttccgc tgcagcatta actaggacta tggcaatttc atcagcagca ttagattcac
    14641 ttccacacaa tggatatatt gttactgtaa caaatggtct ttgtaaagaa acacataagg
    14701 atgcctatat gccaatattc tgggtaactg ttttaacccc atttattgga accatagtag
    14761 cagtcctact gtttacaatg ttcccgggat tgccataaga gaaaagataa aaggtataaa
    14821 ttatattttg atgttataaa atcaattttc aaaatatata caataaattt catgttttaa
    14881 tactgtaaat atgtatttaa aataacaaat tgaccatata aggaggaatt gaaagtgtta
    14941 aataaaccat tagagggagt taaggttatt gacctaacgt attttgtagc aggtccaggg
    15001 acttctaaaa tattagctga ctggggtgct gacgttatta aggtagagcc aagctttgga
    15061 gacccaggaa gaaagacagg ggcaacaatg acaatgccaa tagatgatta taataatcct
    15121 ttttacagca catataattc aaataaaaga ggtttgtcta taaatcttaa gagtgaaact
    15181 ggaattgaaa ttatggataa gcttttaagc gaagcaaacg tatttgtttc aagttataga
    15241 acaggagcat taaaaagact aggtcttgat tatgaatcat taagtaaaaa gcatccacac
    15301 cttatctggg gtcagataaa tggttttggc gattttggac ctgctaagga taatgcagga
    15361 tttgatacag ttgccttttg ggcaagaagt ggggctatgc ttgatatagc agaaaaagat
    15421 acaagtccaa taaatccacc tatagccttt ggagatgcaa caacttcttg tagtttatct
    15481 ggaggaatat gtgcagcttt gtaccagcaa gctaagactg gaaaaggaca aaaagtaatg
    15541 gcatctttat ttgggcaagc aatatggaat gcatcatctt tattagcatc tacacagttt
    15601 agtgatgagt atccaaaaac tagaaaaaat gctatttcac cagtaataaa ttcttatcgt
    15661 tgtaaggatg gaaagtggat attcttaagt attttagagc atgaaagata ttttgaggca
    15721 ctttgtagat tatttggaag agaagactta ataaaagaag agaaatttgc atcttcagta
    15781 gaaagtaaga aaaatgctac agaacttatt aatattttag atggtgaatt tgcaaagttt
    15841 actcaaaatg agatggttga aaaacttact gctgctgata tagctcatga aaaaattcaa
    15901 catgttaatg atgtagtgac tgatgaacag gctattgcta ataactatat atatgaacat
    15961 acaagtaaag atggaaataa gacaatgctt gcatctactc cagttaaatt tggaaatatt
    16021 gaagttaaca tgacttgtga tgctccactt ataggagaac attcagatga gatattgaga
    16081 gaacttggat acagtgattc tgatatagag gctctaattg aatctggaat tgtaacaatg
    16141 aataaagaag catgtgaaat atgttaataa acatgtgaag tatgttaata aataggaggg
    16201 aattttatgg attttagatt aacagaagca caattaatgc ttcaaagagt agcaaaagaa
    16261 tttgcagaga atgaaatagc accaatagct gctgaaacag ataaaacagg tatttttcca
    16321 agggagcttt ttagcaaaat ggctaaaata ggatttaatg gaattggaac acctgtagag
    16381 tatagtggtt ctggtggggc agatatagaa aaggtaattg ttgttactga gattgccaaa
    16441 aaatgtgcag catctgcagc aatattatca atacacacaa tatatgctca agctattttg
    16501 aaatttggta ctgaggaaca aaagaaaaaa taccttccaa tgatggctga aggtgggtgt
    16561 gtaggtgctt ttgccttaac cgaacctaat gctggttcag atgctgcgag agctgcaaca
    16621 actgcaatta ttgatgaaga aactgatgaa tatgttttaa atggaactaa gtgttttatt
    16681 tctggtggag gtcaagcaga atcattaata atatttgcat tgacagaccc atcaaaggga
    16741 ataaagggaa tgtctgctat cattgtcgac aaaggtacgc caggtttttc aataggaaaa
    16801 attgaagaaa aaatgggtat tcatggttca gaaactgcag agttaatatt tgataactgt
    16861 agagttccaa agtctaactt attaggaaaa gaaggtaaag gatttaatat agctatgact
    16921 tgtttagatg gagcaagaat tggagttgga gctcaagctg taggaattgc tgaaggggct
    16981 ttagaagaaa gtatcaaata ttctaaagaa agagtccagt ttggcaagcc aatttcagct
    17041 ttgcaaggta ttcaatggta tatagcagat atggcgacaa tggtggaatc agcaaaatta
    17101 ttagtatatt atgctgcaga tttgaaagct agaggagaaa aacacactaa agaagctgct
    17161 atggcaaaat acaatgcgtc acgtactgca agagaggtta caaatttagc ccttcaaatc
    17221 catggtggat atggatatat gaaggattat ccattagaga gaatgtacag agatgccaaa
    17281 ataacagaaa tttacgaagg tacttcagaa atacacaaag ttgttatttc aagagcagta
    17341 ctaggatagg aggaaaagaa tgaatatagt tgtttgctta aagcaagttc cagatactaa
    17401 tgaagttaaa ataaataaag agacaggtac tctaataaga gatggtgtac caagtataat
    17461 caatccagat gatagaaatg ctttagaaga agctctaaaa atgaaagatg aactaggagc
    17521 agtgattaag gttataagca tgggacctcc tcaagctaaa agtgctttaa aagaagcttt
    17581 ggcaatggga gctgatgaag cttatttaat ctcagataga gcttttggtg gttcagatac
    17641 ttgggctaca tctacaatta ttgctgctgc tatagagaag gttggaaagt atgatgtaat
    17701 attctgcgga agacaagcta tagatggaga tacagctcag gttggaccag aggttgcaga
    17761 atttttaggg ataccacaag ttacttatgc gaaagaagta aaggttcaag atgacaaatt
    17821 actagtaact cgttatacag agacagggga ttatttaata gaagcaaaga tgccagtttt
    17881 attaactgct ataaaagagt taaacaatcc aagatatcca agtgtcaagg gaatcttaga
    17941 agcttataat aatggagatg caaagattac tgtattaaca cttgcagatt tagatgttga
    18001 tacaactcaa attggattaa agggttcacc tacaaacgtt tataagtctt ttgtaccagt
    18061 taaagacaag cataatgaaa taatagaagg tataaataaa aaagaaaaag ctgagaaact
    18121 aatagaaata ctattcgatt tgaaactagt ataggagtgt taagatgatg agagcaaaag
    18181 ttaatcaagg tataaattta aatgattata atggtgtttg ggtaatagga gagcaaaggg
    18241 aaggtaaaat taatcctgtt acgatagaac ttattggtga aggaagaaag cttgctgacc
    18301 aattaggaaa agagcttgca gttgtgatag ctggatatga agtagagaaa gaagtgaagg
    18361 aattacttca ctatagtgtt gataaagtat attacatcaa tgaccctctt cttaaagatt
    18421 tcactactga tggatatgca atttcaattg caaatttaat cgaaagaaaa aagccagagg
    18481 ttgtattggt tggtgctact tctataggaa gagatatagc gcctagaatt gcaggtaagg
    18541 ttggaacagg tctaactgct gactgtacaa aacttgaaat agattcaaca gataataagt
    18601 tattacaaac aagaccagca tttggtggaa atttaatggc cactattgtt tgtccaaaaa
    18661 atagaccaca gatgtcaaca gttcgtccag gagttatggc aaaagctgta agaaacgaat
    18721 cagagactgg aattttagaa gttgttactc cagaacttac tgaaaaaatg attagaacta
    18781 gattagtaga gatacttcca caagaaaaga aatctgtaaa tctaactgat gctagaataa
    18841 tagtatcagg aggaagagga ttaaaaagag cagaaggatt tgagcttata aaagaattgg
    18901 ctgataaatt gggggcagag attggtgctt caagagcagc agtagattca gggtggatag
    18961 aacattctca tcaagtggga caaacaggaa ctactgttag accagaacta tacattgcat
    19021 gtggaatatc tggagcaata caacatttag caggtatgag tgattctaaa tatatagttg
    19081 ctataaataa agatgcaaaa gcccctattt ttagtatatg tgactatgga atagtaggag
    19141 atttatatga aattatacct gaaatgatag aatcattaaa taggtaactt ttagaaaaca
    19201 ttgaatatgt agaatgataa gtcttataat attgtattgt aagacttatt atttttgctg
    19261 aaggttatag tggggagatg aaacaatgaa agagcagtgg tataaggata tatttgcaag
    19321 agtattatct atgactgatg atggttttat tgttgtaaat agaagtggag taataataga
    19381 tattaatgat aaatattgtg actttttagg aaaagaaaga aaagatataa taggacaaaa
    19441 tatacaaagt attataccaa atacaaaaat gctagatgtt atgaaaaata agtattgtga
    19501 agaaggagct atacatcatt attcaggtgg aaatacaaaa gaaaaaagtg taatagtaag
    19561 taggtcttat gtggaaaatg acaatggaga agtagttgca ggagtagctc aagttaaatt
    19621 tagattgcaa tcatttgatg tagctaaaaa gttaatgtca gaatatatgg agctccaata
    19681 ttataaagag cagtttaagg ataactgtgg ttttgacaag ctcataggtg aaaatagaga
    19741 cttcatagaa cttaagaaaa caggtgtgaa agcttctaaa acaaactttc cagtactttt
    19801 aacgggtgaa acaggtacag gaaaagaagt ctttgctaga gcaattcaca ataatagcag
    19861 taggtcagat aaacctatgg ttagtataaa ctgcgctgca ataccagaag aattattaga
    19921 gtcagagcta tttggctatg acgaaggagc ttttacagga gcaaaaaagg gtggtaaaaa
    19981 aggtaaattt ttagttgcga ataatggtac aatcttttta gacgagatag gtgacatgcc
    20041 tttgaccatg caagcaaaac ttttaagagt tcttcaggaa agtgagatag aaccagtggg
    20101 aggtcttaaa actataaaga tagatgtaag agtaatctct gccacaagaa agaatttaag
    20161 taaaatggtt gaagagggat tatttagaga agacctatac tatagattaa atgtaataaa
    20221 cattcatatg atggaattga aggacaggca agatgatatt ttattgctag caaattatat
    20281 attaaacaaa ttaaatgttg aatacaagga attgaaggtt ttaagtgata aggttaaaaa
    20341 ttgttttatt aactatactt ggccgggaaa tataagagaa ctacaaaatg taataaaaag
    20401 tgcctatgct gtaagtgatg atatggtcat tatgatgtgt gaccttccat ctaagatgga
    20461 taatatcagc agagtagctc aatgtaatgt ggatagcaat tgttcaattc atgaaatggt
    20521 tgagaattat gaaaagagct taattattga tgtgttaagg aagtataatt ggaaatgttc
    20581 taaggcagca gaggttatgg gaatacacaa gagtctttta tataagaaaa taaaaaagta
    20641 tgaaattgaa ttaaataatt aagataaatt gaattaaggc aatgctaaaa aaacgccctt
    20701 attcaaagcg aactgtataa actgttttat gcagttaaaa cgtgtaaaag tagatgtgaa
    20761 gtactaatgt caatcattag tactttttta ttatatttat aattactatc tatatttcag
    20821 tatcttttga tatctaagat tattatgttt aatttaggat ataacaaaac cctcttagtt
    20881 atggacagtt ttttatattt taactgtcta ctaagagggg atagtttagt ttaaatttag
    20941 agtaaatttt atctagcatt tataaaaaat attttgaata gcttttttgt tatttaatca
    21001 taattttcta cttctttatc aagttctttg ccacaagaat cacacttata tatagcattg
    21061 tatccgtaat catattcatc aactattttc ttttgtctaa atacaccacc acactcacat
    21121 aggaaaccaa tttttataag tgtaacgata aattgtgatt ttataaattt tatatcatta
    21181 catttatcgt tagtgtaaaa tgtataaata taagagttat cgcactcatc tcttaattca
    21241 aatctttcaa tatatgtgtt attttttaat tcctcaaatt cttctttagt taaatatgat
    21301 ttttgttgca attcatgtaa acgcttaatt tggtccatta tttatatcct cccaataatt
    21361 ttagtatttt aagcttagat aaggaatcta agttttatta aatttatccc aaggaattat
    21421 ttaaatcata tttagtttta ttaattacaa catatataaa ttacacgaaa a
//
LOCUS       DACULQ010000051        21428 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.051, whole genome shotgun sequence.
ACCESSION   DACULQ010000051 DACULQ010000000
VERSION     DACULQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.051"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..2011
                     /locus_tag="JA154_07835"
     CDS             <1..2011
                     /locus_tag="JA154_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HAT7995775.1"
                     /translation="MLHHGNYEDYMRLSISKVQEEIYEYIEKGILEKEEFNLKDIESY
                     INSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDDSVLNYRKFKYILYKGML
                     NLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLEKNNIEFNTIQKRLRELS
                     GRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYRKNIFQNKNDLDTRSSND
                     YIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCVIATVDQLLLSMFKFPGY
                     EKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDVDFHLMTATLPKSYKEQM
                     INKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVSFIKEKEIKSIVEDALEN
                     KQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRFKFKDKKEKYSKILNGEG
                     DIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNNKYEYGNFYILPSEDKIY
                     DNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVKKYFEENFISCDKEIKNI
                     YGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSYKIILEEDFYKEDRELQQ
                     DSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVGLILK"
     gene            2453..3400
                     /locus_tag="JA154_07840"
     CDS             2453..3400
                     /locus_tag="JA154_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAT7995776.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            3419..4177
                     /locus_tag="JA154_07845"
     CDS             3419..4177
                     /locus_tag="JA154_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA methyltransferase"
                     /protein_id="HAT7995777.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            4179..5477
                     /locus_tag="JA154_07850"
     CDS             4179..5477
                     /locus_tag="JA154_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HAT7995778.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            5520..5870
                     /locus_tag="JA154_07855"
     CDS             5520..5870
                     /locus_tag="JA154_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HAT7995779.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            6033..6212
                     /locus_tag="JA154_07860"
     CDS             6033..6212
                     /locus_tag="JA154_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAT7995780.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            6242..6685
                     /locus_tag="JA154_07865"
     CDS             6242..6685
                     /locus_tag="JA154_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAT7995781.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            6796..7293
                     /locus_tag="JA154_07870"
     CDS             6796..7293
                     /locus_tag="JA154_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAT7995782.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            7486..8049
                     /locus_tag="JA154_07875"
     CDS             7486..8049
                     /locus_tag="JA154_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal subunit interface protein"
                     /protein_id="HAT7995783.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            8256..8498
                     /locus_tag="JA154_07880"
     CDS             8256..8498
                     /locus_tag="JA154_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HAT7995784.1"
                     /translation="MLMLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIK
                     TKVKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            8511..9854
                     /locus_tag="JA154_07885"
     CDS             8511..9854
                     /locus_tag="JA154_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein"
                     /protein_id="HAT7995785.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            9892..10908
                     /locus_tag="JA154_07890"
     CDS             9892..10908
                     /locus_tag="JA154_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAT7995786.1"
                     /translation="MIIQKKFTIADGNFERELFGNFDENVKLIEKTLNIDVILREGNI
                     ILIGEEKNVDSALKLMNELHQTVSNGKHLDKQSISYSLSLLLEGSEQKIKELEGTIVI
                     TQRGKAVQPKTLGQKEYIKLIENNDITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRII
                     LTRPAVEAGESLGFLPGDLKDKVDPYLRPLYDALFEMLGADKFNKYLERGTIEVAPLA
                     FMRGRTLDNSFIILDEAQNTTSEQMKMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLI
                     QATEILKGVPGIGSIMLTDRDVVRHELVQRIIRAYEKHDKREEFKKEERKKAKMQEKT
                     FKRK"
     gene            10965..11426
                     /locus_tag="JA154_07895"
     CDS             10965..11426
                     /locus_tag="JA154_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAT7995787.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            11432..12151
                     /locus_tag="JA154_07900"
     CDS             11432..12151
                     /locus_tag="JA154_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAT7995788.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            12270..12671
                     /locus_tag="JA154_07905"
     CDS             12270..12671
                     /locus_tag="JA154_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAT7995789.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            12735..13628
                     /gene="era"
                     /locus_tag="JA154_07910"
                     /gene_synonym="bex"
                     /gene_synonym="rbaA"
                     /gene_synonym="sdgE"
                     /gene_synonym="yqfH"
     CDS             12735..13628
                     /gene="era"
                     /locus_tag="JA154_07910"
                     /gene_synonym="bex"
                     /gene_synonym="rbaA"
                     /gene_synonym="sdgE"
                     /gene_synonym="yqfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088950.1"
                     /note="Era; Escherichia coli Ras-like protein; Bex;
                     Bacillus Era-complementing segment; essential protein in
                     Escherichia coli that is involved in many cellular
                     processes; GTPase; binds the cell membrane through
                     apparent C-terminal domain; mutants are arrested during
                     the cell cycle; Streptococcus pneumoniae Era binds to RNA
                     and Escherichia coli Era binds 16S rRNA and 30S ribosome;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAT7995790.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            13631..15019
                     /locus_tag="JA154_07915"
     CDS             13631..15019
                     /locus_tag="JA154_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAT7995791.1"
                     /translation="MGYMDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFD
                     IMENLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDM
                     ADKLSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEA
                     IDHMREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVD
                     EDREEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHE
                     REVAEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFI
                     PVVIGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVM
                     RKLDIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVY
                     FIITTALLSKIV"
     gene            15092..15247
                     /locus_tag="JA154_07920"
     CDS             15092..15247
                     /locus_tag="JA154_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HAT7995792.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            15213..15986
                     /locus_tag="JA154_07925"
     CDS             15213..15986
                     /locus_tag="JA154_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAT7995793.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            15976..16578
                     /locus_tag="JA154_07930"
     CDS             15976..16578
                     /locus_tag="JA154_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995794.1"
                     /translation="MMSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYL
                     ENKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIP
                     LTVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMIT
                     NAAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            16738..17616
                     /locus_tag="JA154_07935"
     CDS             16738..17616
                     /locus_tag="JA154_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAT7995795.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            17616..19682
                     /locus_tag="JA154_07940"
     CDS             17616..19682
                     /locus_tag="JA154_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAT7995796.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKILIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(19848..21395)
                     /locus_tag="JA154_07945"
     CDS             complement(19848..21395)
                     /locus_tag="JA154_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HAT7995797.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
BASE COUNT         8637 a         2179 c         3907 g         6705 t
ORIGIN      
        1 aatgttacat catggaaact atgaagatta tatgaggtta agtattagta aagttcaaga
       61 agaaatttat gagtatattg aaaaaggcat tttagaaaaa gaagagttta atctgaaaga
      121 tatagaaagt tatataaata gcattttaaa tgtagacttt aaatttggtg aagatgtatt
      181 ggattatgat tacatagata aattaagtga aagtatgttt atagacagtg attataacaa
      241 tggtcaaata gatgattcag ttttaaatta tagaaagttt aagtatattt tatataaagg
      301 gatgctgaac ttaatagacc atagtgcatc aagtagacag aaaggtataa aattttataa
      361 cgattttact gatgaagaaa ttgataatat gattttaaat gagatatata agagtcaggg
      421 taatttggaa aaaaataata tagagttcaa tactatacaa aaaaggttga gagaactttc
      481 tggaagaaat gttctgactg ttgcatttac aggttcagga aaaacagctg cagattatag
      541 aaagactttt aaaagaaaat tctttttagt gccaaataaa atatcagcag aaagttttta
      601 tagaaaaaat atttttcaaa ataaaaatga tttagataca agaagtagta atgattacat
      661 tggattactt catggagata taaacttata ttcagagaat gaagataatg gagaacatga
      721 ttttgtattg acattaaggg atatagattt gagtataaac ttttgtaaac cttgtgtaat
      781 tgcaacagtt gaccagttat tacttagtat gtttaaattt ccgggatatg aaaaaatatt
      841 cgcagccgta aaaaatgcat ctataactgt tgatgaagta catttattgg aaccaaagat
      901 gtttttgatt ttgatatatt ttatgcagtt tgcatgcaag tacttagatg ttgattttca
      961 cttaatgaca gctacacttc caaaatcata taaagagcaa atgataaaca agggaattat
     1021 ttttcaagaa gaaagtaatg aaaatgttac tgataaagga gaaatagttt ttattgagag
     1081 caacaaggaa gaagagattt gtgaaggtaa agatgtaaag gtttctttta taaaggaaaa
     1141 agaaatcaag tcaatagttg aagatgcttt agaaaataaa caaaaaattc ttataataaa
     1201 gaatactatt gattcagtca atagaacata tgagttttta aaagaaaatt tatctgataa
     1261 gtatggtggt gtagatatag atgttttaca tagtagattt aagttcaaag ataaaaaaga
     1321 gaaatatagt aaaatattaa atggagaggg tgatatttgg atatcaactc aatcagtaga
     1381 aatatccttg gatttggatt tcaatataat aataagtgat ttagcaacta tggatagctt
     1441 gatacaaaga atgggtagat gtaatagaaa taataaatat gaatatggaa acttttatat
     1501 tctacctagt gaagataaaa tttatgataa caaacttaag aacacaacta aaaacatctt
     1561 aaaggacata ttaaaaacta actctatttt tacaatggga attagaaaaa ctattttgga
     1621 tgattactat gataatagtg tagtcaaaaa atattttgaa gagaatttta taagttgtga
     1681 taaagaaatt aagaatattt atggtataaa taaagaaatt tttgatggat tggatttaat
     1741 ttttaatttt gaaccttaca aaaatattgt tgatagtaag aaggaagcag ctaaaatttt
     1801 tagagatgtt gatgttagtt acaaaattat tctggaagaa gatttttata aggaagatag
     1861 agagttacaa caagattcga ttcaagtatc agggtttata tttaatagat tatattatta
     1921 tgggttaata agcaaagtag aaggatacat ggtactaaaa tctagtagta agtttgagta
     1981 taatagtact gtaggcttaa ttttgaagtg agcatatgtt taaatataat atacctttag
     2041 acattgaaaa tacaaaaagt actactgtaa gctcactgta aaaactattt ttttaaaagg
     2101 aaaaataagt tgtgagatag agtgtttaca agtgtatata gataataaat ttgaggttta
     2161 agattaacta agtgggtttc aggtacatcg cgaaaataat tataagttgg agattttgta
     2221 tgtttaagat taactaagtg gatttcaaat attgatatag tagatataat agaagtttta
     2281 ttattaggtt taaaattaac taatcggatt tttaaatatt ctgactatta tataaggata
     2341 ttattagctt gaatttaaaa ccaataatat aaaacaacaa aatattatat ttatgatata
     2401 ataaaagtta tggaaaaaca ggaatattac tatagagaag agaggtaaac ttatgaataa
     2461 ttggatagaa gtaactataa aaacaacaac agaagcagtt gaacctataa cgaatatact
     2521 atatgaacaa ggagctggag gagctgtaat agaagaccca aaagactttt tattccaaaa
     2581 gaaaaatgaa ctagactggg attatgttga agaagaagta tttaaaaaga atgaagaaga
     2641 tgatgttctt ataaaaactt atgtttctga agaaaaaaat gtgatggaat ttgtcgaaat
     2701 aataaagcaa aaagttttag ggttaaaaga ttttggtata gatataggtg aaggaagtgt
     2761 atcattggac caagtaaatg aagctgattg ggcaaatgca tggaaagcat actacaaacc
     2821 aacaaaagta ggacaaaggg tagtagtaaa gcctacatgg gaagattatg ctatgcaaga
     2881 gggggattta atcatagagc ttgaccctgg aatggcattt ggtacaggta cacatgagac
     2941 tactagtatg tgcattagag aattagaaaa gtacgtgaat aaagattcta aagtatttga
     3001 tattggctgt ggtagtggaa tccttgcaat agcagcagct aaattaggag caaaagaagt
     3061 tgtagctgta gatttagatg aagtggctgt aaaagttgca aaagaaaatg ttcttgaaaa
     3121 taaagttgaa aaaagtgtat ctgtaatgca tggtaattta actgatgtaa taaaagataa
     3181 agcggatgta atagttgcaa atataatagc tgatataata aagatacttg caaaagatgt
     3241 tcaaaacttt atgaaagaag atgctatatt catatcttca ggtataattt tggataaagt
     3301 agaagaagtt aaagaaagct taattgaaaa tggatttgaa atagttgaag ttcaaaaatt
     3361 aggtgaatgg tcagcaatag tttctaagct taaaaaataa gggatatggt gacgtataat
     3421 ggatagattt tttgttgaaa aaaataatat aaatctacaa gataaaactt gcactataga
     3481 aggtgaagat gtaaaacata tttcaaaagt tttaagatgc aaattaggtg agaaacttga
     3541 aatatgtgac aagaataata atgagtatat ttgtgagata atgaatatag ataagagtat
     3601 agttaattta gaaattttag aaaaagttga tataaataga gaatcagaac ttaaagtaag
     3661 gctataccag ggacttccaa aagctccaaa aatggaaatg atacttcaaa aattaactga
     3721 agtgggtgta gaagaaataa tattagttca aactaagaga agtgttgtta aagttgatga
     3781 caaaaaagaa gataaaaaat ttgaacgttg ggaaagaata atttatgaag cagcaaagca
     3841 aagcaaaaga ggtaagatac caaaattaag aggtgtttta agttttaaag aagctttaga
     3901 agatatgaaa aaaaataatg taaatatttg cccttatgaa aatgaaagaa cagtttcaat
     3961 aaaacatgca ctaaaaaaat gtgattctaa tatagatagt gttggaattt ttataggtcc
     4021 agaaggtgga ttttctgaag aagaaataga acaaatacaa aaaaataatt gtaatgttgt
     4081 atcattgggg cctagaatat taagaactga gacagcttct gttgtagcat caacaatagc
     4141 tttgtatgaa ctaagtgatt taggaggaga aaaataaatt gaaaaaagta gctttttata
     4201 cacttgggtg taaagttaat caatatgaaa cagaagctat gctagaacta tttgagaaag
     4261 atggttatga acaagtcaat agtgaagagt atgctgatgt ttatgttata aatacatgta
     4321 cagttactca tatgagtgat agaaaatctc gtcaatatat aagacgagtt aaaaagaaga
     4381 atccagatgc aataatagcc gttgtaggat gttattctca ggtatctcca gaagaaattt
     4441 tggatataga agaagtaaat ttagtcatgg gcacaaatga tagaagaaag attgttgaag
     4501 aaattaaaaa gataaattca agtaagaaag ttagtacagt tgatgacata atgaaagtta
     4561 aagcttttga agaaatagaa atcagccaaa ctaatggaaa gactagagca tttatgaaaa
     4621 tacaagatgg atgtgacaga ttttgtactt attgtatcat tccttatgcc cgaggacgcg
     4681 ttagaagtag agatattgat agtatagtcg atgaagtgaa aaaattggct aataatggct
     4741 ataaagaggt tgtattaaca ggaatacatg ttgcctctta tggaaaagat ttaaaggata
     4801 gggatattaa acttcttgat gtaataaaac aaataaacca gatagaaaaa atagaaagaa
     4861 ttaggcttag ttctgttgaa ccaattttat ttacagatga atttgtaaat gaagttttaa
     4921 agatggataa agtttgtcct cattaccatt tatcacttca aagtggatgt gatgaaactc
     4981 taaaaagaat gaatagacga tacacaactt tagagtacaa aactattgta gatagattga
     5041 gaagtaaaat gccagatgtg gcaataacta cagatgtaat agtaggtttt cctggagaga
     5101 caaatgaaga gtttaaaaaa acgtacgagt ttttaaaaga aatagagctt tcacaaatgc
     5161 atatttttaa atattcacca agaaaaggaa ctccagctgc aactatggaa aatcaagttg
     5221 accctcaaat gaaacacttt agaagtgaac aacttttaaa tttaagtaaa gtaaatttta
     5281 ataaatttgc aactaaattt atcggccgtg agttggatgt tttatttgaa caaaatatag
     5341 aaggcaataa atatgaggga ttaacttcaa attatataag agttgttgtt gaaagtgaca
     5401 aaaatataca aggtcagata ttaaaagtga aaataaatga tgtaaaagat gaatatgtag
     5461 aaggaatttt gctataacat gtagaaatta taatatttat aagataggag gtggaaagaa
     5521 tggattgtat tttttgtaaa atagcgaacg gagaaatacc ttcaactaaa gtttatgaag
     5581 atgatagagt cttagctttt aatgacttga acccagttgc tccatatcat atattagttg
     5641 tacctaaaaa acattatgat agtttaattg atattcctga taaagagatg gacattgtgt
     5701 cacatattca tgttgttatt aataaaatag ctaaagagaa aggttttgac caaactggat
     5761 tcagagtcat aaataattgt ggaagtgatg gagggcaaga agttaaacat ttgcactatc
     5821 atattttagc tggtaaaaag cttcctaatt acgaagcggg acaaaactaa actaaattat
     5881 tgaaaaattg agttccttct tgataaaaat acttgataaa agtaaaaatt tagtttataa
     5941 taaacaaagt gttaggttgt aatgcatatt atgcaaaaag aacatagtta cagtcccagc
     6001 ataattgcgc tattggaggg agggaaaaag aaatgtcaga agtaagagtt agagagaatg
     6061 aaacattaga cagcgcttta agaagattca agcgtcaatg tgcaatgtct ggcatcatgt
     6121 cagaagtgag aaaaagagag cactatgata agccaagcgt taagcgtaag aaaaaagctg
     6181 aagcagcaag aagaaaaaac gctaagaaat agtagtaaat aagaataaag aggtgaaggg
     6241 gatgtccctt aagcaaaagt tacaagagga tttgaaatct tcgatgaaaa acaaagatac
     6301 agtgagaaaa tctgtagtaa ctttaataag agcttctata aagcagtatg aggttgataa
     6361 tagggtcgag cttgatgaag atggaattat cgatgtaata gcaaaacaat taaagcaacg
     6421 tagagatgca ttagttgaat ttgaaaaagc tggaagagaa gacttaataa aagaaacaga
     6481 aggtgaaatt gaagttttaa aagagtacct acctcaacaa ttaagcgaag aagagttaga
     6541 agaaattgta aaatctacta tatctgaagt aggggcaact tctatgaagg atatgggaaa
     6601 aataatgtca gttatccaac caaaagttaa aggaagagct gacggaaaac ttataaataa
     6661 gttggtaaaa caaaacttac aatagattga tatagcctag agagaaatct ctaggctttt
     6721 tatagttttt gggaataacg agtcatattt gagcatatct ataatcaaag aaaattttga
     6781 aagaagggga aaaatttgat tatatcagta aaagagatta ggaatttttt gataaacata
     6841 tttataccac taataatagg ttacttaagt agcatcttaa caatgattat cagtggaaca
     6901 gatatttcaa cctattattt acaactcaca aagccaagct ttgcaccacc agcttttatc
     6961 tttcctatag tttggactat attgtacata ttgatgggta tatcttcata cctaatatta
     7021 agaaagggtt acaacttacc taaagtaaga gatgcaattt tttattatgg attacaactt
     7081 gtattaaact ttatttggag tatactattt tttggatttg gacttagatt tacagctttg
     7141 atagatataa taataatgat tttggtttct ataataatga tttcgaagtt ttctaaaata
     7201 gataagagat ctggatacat aaatttgatt tatttaatgt ggcttgttta tgcaggattt
     7261 ttgaactatt ttatatggtt tattaataaa tagaatgtaa agattttgta aattaccctg
     7321 tacttttaat aatatcataa caaacgaaaa ataaatagac tattaaaaat aatcattttc
     7381 atatacaata atattaattt aaatacaata aatttataaa aattgtattt aaagattaac
     7441 atattaaagt attagaattg gaaaggcttg aagggagagg tttttatgca aataatagta
     7501 tctgggagac aaatgaaatt aactgacgga ataaaaggat acgtagatgg aaaattaagt
     7561 aggttagaaa aataccttga tcctgaatca gaagtgaaag taacagtaag tgcgaaaaag
     7621 gatagacaaa aagtggaggt tacaataatt cctattaacg gtcaaataat aagagcagaa
     7681 gacgtagaag atgacttata tgcagcaatt gatattgttt gtgacaaatt aagtagacaa
     7741 gttgtcaaat ataaaactaa agttaaagac aaagttcaaa ataataagag cataagattt
     7801 gaaaatcttg actttataga taatagcagt gaatttgatg attatgatta tgatgatgaa
     7861 gaagatgaga atatagtaat agaaagaaga aagaaattta atgtaaaacc tatgagttct
     7921 gaggaggcga tacttcaaat ggaactagta ggacacaatt tctatatgtt tagaaaccaa
     7981 gataattttg aaataaacat agtgtataag cgtaaagctg gtggatatgg tttaatagaa
     8041 caagattaat aaaaattgta tacgtttaga ttttatataa gaaaaaataa aaaggtaatc
     8101 atgttgattt tttatcaata tgattacctt tttcttgttt aaataatata ttagatatta
     8161 ggtatttatt atataaaaat tttaaaacta atctgttaca acatcaataa agtgcataaa
     8221 tacctatttc aagcataaaa ttaattaagg gggtaatgct tatgttagaa atatccactg
     8281 atttacaagt aaatcaacca gttattactg ttacaagtaa tacgtttatt agtatagaaa
     8341 attatctatc aatattagaa tatgaagttg atttaataag aataaagact aaagttaaaa
     8401 caattaaaat atctggggat aaactttcac ttaagtatat tactgatagt gagataggta
     8461 taaaagggat tatctacaat gttgagtatg ttgattagga ggagatacac ttgataagtt
     8521 ttataagggg atactatgtt atagttgtgg aaggagtagg actcgaacaa tttttaaatc
     8581 atcttataag aaatgggata agtgtttaca atgtaactag aataaaaaat actaaaatgg
     8641 agtttcatat agatagacaa gatataaaag aatttaagaa tgtttataga ggaagtaagt
     8701 ttgatataaa ggtaaagcaa aaaactgggg tcccttttat tatcaaaaga gtttataagc
     8761 acaaaggtat gtggatttgt gctcttgttt ctttattttt attgatgtct acatcacagt
     8821 ttgtgacaga tgtatatata caatctcctg aaggtataaa aaaagaagct cttagaaatg
     8881 agctttataa ggttggagta agaccaggag tctataagaa gagtattgat agaaaagaag
     8941 ttagagacca tatgatgtca aaatttaatg atgtggcata tttatctatc aatgtaaaag
     9001 gaacaaatat atttgttact gttacaaaaa aggcagaatc cctaaaatct acagaccaat
     9061 caaattattg taatgtaatt gctttaaaaa atggtataat agaaaaggta atccctagaa
     9121 gtggtaaatc agttattaaa tctggagaca tagtccaaaa gggagatgtc ttattaaatg
     9181 gagctaatac caagtcaata ccagaagtat gggcaagtac tttttatgag tccacaaaaa
     9241 aggcaagtta tgtagatact gtaaataaaa aaactggaga aaagaaaaat atatatacac
     9301 taagttttta tgataaagag tttacaatga gaaaaaatat aaaatataaa gattatgttg
     9361 ttgaaaataa agaaaaaaaa ctatcaatag gaaactatac ttttccaata aagataaaaa
     9421 cgagtacttt ttatgaaaca aaaaaggttg aagtaaaaag gaataaagaa gagttaaaaa
     9481 aagaattatc tgaaaaagct ttaaaagagt tagaatacat aatcccagct tcggctagaa
     9541 taatagatgt aaaacataac tttaaggtta ataaaaatat gttagaatat ttaataacag
     9601 ttcaaactag tgaaaatatt gctaaaattt atcctcttag taagtcagaa gcagaaaggt
     9661 ttataaagga agaaagtaaa ccagatgaag gtgaagagga agttccttcc aatccagaaa
     9721 aaagaccatt aaatgatata agaaatgagt ttgatgagga taataaggat aataaagatc
     9781 aaaacaattc tgatgaaaat aatagtaatc aaaatagtaa taatagtcaa aataataata
     9841 gcaataacaa ctaataaaat taaacttaaa atcttaggag gactaattca attgataata
     9901 caaaagaaat ttacaatagc agatggtaac tttgaaagag agttatttgg caactttgat
     9961 gaaaatgtaa aattaataga gaagacttta aatatagatg taatacttag agaaggcaat
    10021 ataattttaa ttggagaaga aaaaaatgta gattctgcat taaaacttat gaatgaatta
    10081 caccaaactg tatcaaatgg taagcattta gataagcaga gtatttctta ttcattatct
    10141 cttttgcttg aaggaagtga acaaaaaata aaagaacttg aggggacaat cgtcataact
    10201 caaaggggaa aagctgttca acctaaaact ttaggtcaaa aagaatatat aaaacttata
    10261 gaaaataatg atataacttt tggtgttgga cctgctggaa caggaaagac ttatcttgcg
    10321 gttgctatgg cagtaaaagc ttttaaaaga gatgaagtta gtagaattat acttacaaga
    10381 ccagctgtgg aagcaggaga gagtctaggt tttttaccag gagatttaaa ggacaaagtt
    10441 gacccatatt taagaccact atatgatgcc ttatttgaaa tgttgggtgc tgataaattt
    10501 aataagtatt tagaaagagg tactattgag gttgcaccac ttgcatttat gagaggtaga
    10561 acacttgata attcatttat aatattggat gaagctcaaa atactacttc tgaacaaatg
    10621 aaaatgtttt taactagact tggatttggc tctaaagctg tagttacagg agatgtaact
    10681 caaacagatt taccacaaaa taagaagagt ggtctaatac aagctacaga gattcttaaa
    10741 ggtgttccag gtattgggtc tataatgcta acagatagag atgttgtaag acatgagttg
    10801 gtacaaagaa taataagagc ttatgagaag cacgacaaaa gagaagaatt taaaaaagaa
    10861 gaacgtaaaa aagcgaaaat gcaggaaaag actttcaaaa gaaagtaaaa gtgtagtaat
    10921 atagatatat agcattatta atgaataaaa agtaggtgta gtgaatggat ttgatactag
    10981 atgatagaca agataaatta gaagtaagtg aggagttaat tgaaaagata aaggatataa
    11041 tcatagaatg tttagattat gaaggatatg atgataatta tgaagtgagt ctttcttttg
    11101 tagataataa agagattcat gaattaaata gagaatatag aggagttgat agagtgactg
    11161 atgttttatc atttcctctt ttaagtgatg attttgagga tgttgagtta gaagaagaat
    11221 ccctaggtga catagtagta tctcttgaaa gagccttaga acagagtata gagtataacc
    11281 atagctttga aagagaagta tgttttttaa tatgtcatag tatgtttcat cttttaggtt
    11341 atgaccacga tacagatgaa aatacaaaag aaatgagaga aaaagaagaa cacatattaa
    11401 ataagttaaa cataacaagg gagtagtttt tatgaaacca gaaaaaactc gtcaaggtat
    11461 aattaaagca tttaatgcag ctatagaagg gatattgtat acatttaaat ttgaaagaaa
    11521 tatgaaaata cattatttag gttcagttgc tgttcttata ataagtttgt tttttaactt
    11581 ttcaaaatta gaaatgataa tgttacttat gtccatatgc ttagttgtag ttgctgaaat
    11641 gtttaataca gctatagaaa aagctgttga tttagtaact gatgaatatc atgttcttgc
    11701 taagatagca aaagatgttg cagctggagg cgttctagtg gcagcactaa attcagtagt
    11761 agttggatat attttgtttt atgataaatt gacagatata tctggaatat taatttataa
    11821 aataagagaa tcagaattac atataacctt gatatgtata ttattggttc ttatagctgt
    11881 tgtagttgta aaagcattga cttctacagg tactcctcta aaaggaggaa tgccaagtgg
    11941 tcatgctgcc ttagcatttg ctatagcaac tgcaattaca cttatgactg aaagagttgt
    12001 tgcctctact cttgcatata taatggctgt attagttgcc caaagtagaa ttgaaggaaa
    12061 aatacatacc ttttgggaga ctattgctgg agctttgtta ggtgtgttga tagctatatt
    12121 agtatttcag cttggaatgt tctataatta aaataaatgt acataaaata tttaaaataa
    12181 aacaaatctg tgctaattaa atttttatga tataatgtag agtattaaat aaaaataact
    12241 tttaattatt gtatacagga ggagttgttg tggataacat agaattgtta aggttggcag
    12301 aagatgccag acaacattct tatgctcctt actcaggttt tagagtaggt gcagctcttt
    12361 taactaagag tggtaaagtt tatactggat gtaatataga atgtgcatca ttaggtggaa
    12421 caaattgtgc agaaagaaca gctattttta aagcaatatc agagggtgat aaggatatct
    12481 ataaaatagc tatagcaagt gataactcag aaaataatga gcaaacatat ccatgtggaa
    12541 tatgcaggca ggtaattata gagtttggtt ctgatataaa aataataacg ggatatacta
    12601 aaggagaggt gtttgagcat agtataaagg atttgttgcc aaactatttc tctggaaatg
    12661 atttaaaata aatttaaaag tataaataaa tttaagtaat taaaacaaga agataaaaag
    12721 ttaggagaca gttaatgttc aaatcaggtt ttgtcagtat agtgggtaga ccaaatgtag
    12781 gaaaatctac tttaatgaat aatgtagttg gagaaaaaat agctataatg agtgataagc
    12841 ctcaaactac aagaaataca atacaagcag tatatacaga tgaagaaatg cagatagtat
    12901 tcttagatac accaggtatt cacaaaccaa aaaataaatt aggtgaattt atggtaaagg
    12961 cagcaacaga agcttttaaa aatgtagatt taatattatt tgtagttgat gattctaaaa
    13021 aaataggtcc tggagataga aagataatag aggatttaag aagtgttaag acaccaataa
    13081 ttttagttgt aaacaaaata gaccaactag accaaaaaga tgagctattt gatataatca
    13141 aaatgtatga tagagaaggc atttttaaag aaatagtacc aatatcagca ttaaaaggaa
    13201 agaatacgga taccttaata aaagttatac aaaattattt agaagaaggg ccaaagtatt
    13261 ttccagatta tatgattaca gaccagccag aaagagtatt aatagcagaa cttataagag
    13321 aaaaagttct tcactatctt aatgatgaaa tacctcatgg tgtagctgtt gaaattgaaa
    13381 aaatgaaagc gagaaatgat aaggaaatag tagatgtatc tgctgtaata tactgtgaaa
    13441 gagattcaca taagggaata ataataggaa aaaatggtag gaaactaaaa ggaataggta
    13501 agtcagcaag acaagatata gaattgcttt taggttcaca aataaatctt caattatggg
    13561 ttaaagtcaa agaaaattgg agaaatttac aaaattatat aaataatttt ggatataatg
    13621 ataaataaag gtgggttata tggataggaa actggatgct tcccaagatt tattatatga
    13681 agtaaaatca ttaattgata ataataaagt attggagtta agagaattaa tagaggaata
    13741 tcatataatt gatatattcg atataatgga aaatttagag gaagacatga aaattcaatt
    13801 atttgaagta cttcctttag atatggcatc ttctatatta gaagaaggta gtgtagagtt
    13861 ttttattagt attttatcta aactggatgt agaacactcg aaaaatatac ttgaacttat
    13921 gtctcttgga gatatggcag acaagttgag tgaacttgaa gaagaagaaa gagaacacat
    13981 aataaatctt ttgaatcaag agaatgccga ttatgtaaaa gagttactat tctatgatga
    14041 agattctgct ggtggaacaa tgaccactgg atatatttct ataaataaag atatgacagc
    14101 tcttgaggct atagaccata tgagagaaga agctgaagaa gctgaaacta tttattatat
    14161 atatgtggtt gatgatgaag aaaaactagt tggagtattg tcattaagag aattaataat
    14221 agctagagac gcaaatatag tagaagattt gatgagtgaa aatataatct ctgtttatgt
    14281 agatgaagat agagaagaag cagtaagatt agtatctaaa tacaatttaa tagcaattcc
    14341 agtagtagac agacaagaaa aattaaaagg aataataaca gtagatgata taattgatgt
    14401 aatggaagaa gaagcaactg aagatatgta taaatttgca ggaagttcag aacatgaaag
    14461 agaagttgct gaaaaagaaa accctacatt gagagagcaa ataatatcag cacttagagg
    14521 acgattacct tggttaatta ttaccttagt tggagggttg ttggcttcgt taatactttc
    14581 taacttagac tatataatga atcctgtata tgctccatta gtatttttta tacctgtagt
    14641 aattggtatg ggtggaaata ttggaactca atcatcatct gttacagtaa taacattatc
    14701 taataaggat ttaaatttta gcaatgtggt tagagaaggt attgtaggta ttattacagg
    14761 tcttttgtgt agtataataa caggtattgt aatttacttt gttatgagaa agttagacat
    14821 tgtattaatt gtttcaatat cattatttat aaatatggtt ttaggtgcaa caataggtgc
    14881 gtttatgcct gttttattaa aaaaaatgga tgcagaccca tctacagttt catcaccaat
    14941 tatatctaca gctcttgata taactggtat agcagtttac tttataataa caacggcatt
    15001 attgtcaaaa attgtttaat aactttatgt atgatttatc ctcttcttac taaaaatata
    15061 agtataaatg taagaagggg gttttttaaa aatgaacaat atcagttggg aagtttttaa
    15121 aaaaacaggt agtatagatg catatttata tttctgtgat tgcaaaaaca tacatgaaga
    15181 aattgaagaa gtaagggaga aaaaagaaga cgatgataat cctgaacacc caggggatag
    15241 tacttaaagc tataaggtat aaggaaagtg atataatact tacactattt acaagaaagc
    15301 ttggaaaagt atctgcaata gcaaaaggag ctaaaaaaaa taagagctct ttgctttcat
    15361 catcacagtt attttcttat agcaatttta ccttaaaaaa acaaggtaat atgtacaagg
    15421 ttactcaaag tgaaataata aaaagttttt ataatatatc ctatgatata gaggcatttt
    15481 cttatgctac atatattaca aaattagttg aaaattctat acttgaaaat cagactaata
    15541 ataggctatt tatattactt gcgcagactc tgtatttgta tacacaggat aatacagaca
    15601 ataggtttat aacagcagca ttcgagttaa aatttttaga ctatatagga tttaaaccta
    15661 tagtaaataa atgcattaat tgtgaatgta aaattttaca aaattctgtg tttaatatct
    15721 acgaaggtgg tatactatgt agtaagtgta gtaaactttt tgataataat ataaaattag
    15781 acttaacaac tataagtctt atggaatatg tattaagaaa tgatatttta acatgtagta
    15841 aagctaaagt atcaaaatat ataacacatg aacttgagaa tattctagac aagtatttga
    15901 aagtttatgt tgataatatt aattttaaat cattacacgt attgcaaagt gtaaaaaata
    15961 ataagggagt ggataatgat gagtagtaat ataactattg aaatggttga ttcagtcttt
    16021 gaaagagttc caggagcaac ttatgcagaa gcaaaagaag cattagtaat gtgcaatgga
    16081 gatgtaattg aggcagtaat atatttagaa aataaaaaaa atacttgtaa ttgtaaagca
    16141 aaatctgcta aagaaactat ggaagaagta tttggcaaag atggagaaaa tataaagttt
    16201 caattaaaag agcttcttag aaaaagtagt gtagtgagaa taatagttga aaaagatgga
    16261 aaaactatga tgaatattcc tttaacagtt ggagttgttg gattagcttt tgtaccatta
    16321 gctactttag ttggattatc agctgctgtt ataagtaaat atcgtataaa agttcaaaat
    16381 gaagaagatg gagaaattgt agatttaggt gaattgaatg aagaaaaact tcacgttttg
    16441 aagaatatga taactaatgc agcaaaagat gtgaaggatg tagtagttga caaaaaagaa
    16501 gatgacaaag atgtaactgc tgaccttatg aaagaagaat ctgaaaagaa taaagaagac
    16561 gaagacgaaa ataaatagat ataaagtgac ttttaaaaaa tgaagagaag ataattataa
    16621 gaattatcac taacattgac aaattttata gaatttgata ttctaaaagt tgtagaagaa
    16681 ctattaaaat agcctttcgt gtagacgaga ggctttattg ttataggagg gatttttatg
    16741 aattttcaag agatgatact ggcgttgcaa aaatactggt caaagcaagg ttgtatcatg
    16801 atgcaacctt atgatattga aaaaggtgcg ggaactatga atcctaatac ttttttaaga
    16861 tcattaggac ctgagccttg gcaagtatgc tatgtagagc cttcaagaag accagcagat
    16921 ggaagatatg gtgaaaaccc aaacagacta tatcaacatc atcaattcca agttatacta
    16981 aaaccttctc cagataatat acaagaatta tatttggaaa gtttgaaaga gattggaatt
    17041 gacccaagtg agcatgacat aagatttgtt gaagataact gggaagctgc tacagttggt
    17101 gcttggggac ttggatggga agtttggcta gatggtatgg aaataactca atttacgtat
    17161 tttcaacaag taggtaatat tgaatgtgag cttgagacag gagaaattac atatggatta
    17221 gaaagattag ctatgtacat tcaagaagtt gatagtgttt atgatttaaa atggaatgat
    17281 aaaataactt atggtgaagt atttaataaa gctgagtatg aaaactcaat gtacgcattt
    17341 gaattatgtg atgcagatat gttgtttaat ctgtttgata tatatgaaaa agaagcatta
    17401 agacttatgg aaaatggcct tgtaataccg tcatatgact atgtgttaaa atgctctcat
    17461 gcatttaata cattggatgc aagaggtgca ataggagtta gtcaaagagc ttcatttata
    17521 ggtagagtta gaaatatggc aaagactgtt gcagaaactt ttgtaaaaca aagagaagaa
    17581 atgggattcc ctcttttaaa ggacggtgac aaataatgaa aaattatctt ttatttgaaa
    17641 tcggtgttga agaaatgcca tcaagatttg taggcagtac actagaacaa ttaaaaaaca
    17701 atcttagtaa attatttgat gaaaatagaa tagcttttga taaaataaat gcttatggga
    17761 caccaagaag acttacttta gtagtcgaag gattgagtga tagacaaagt gatttagaag
    17821 aggaaataaa gggtcctgct aaaaaaattg ctgttgatgc agaaaataat ttaacaaaac
    17881 cagcattagg atttataaaa agcaaaggat taagcgaatc tgacatatat tttaaacaag
    17941 ttggaaaaga agaatatata tttgcgacta taaagcaaga tggaaaagaa acttcagaag
    18001 tactaaaaga catattacca gagactataa aatctgtagt attcccaaaa gctatgcgtt
    18061 ggggaggaaa aaatcttaaa tttgcaagac ctataagatg gatagttgca ttgttaaatg
    18121 ataaagtatt agagtttgat ttagaaggaa taatatcttc taatgtaaca aaaggtcata
    18181 gatttttagg tgaaagtaca atagaagttg attctataga agattatttt gaaaaattag
    18241 aaaaaaatta tatagtttta gaccaaaaca agagaaaaga aatgataaga aaacaatgta
    18301 tagaagtagc aaattcttta ggtggagaag ttgaatttga tgaagattta cttgaagagg
    18361 taacttattt agttgaatat ccaactgctt tttatggaga atttgatgtg gattatataa
    18421 aactaccaaa agaagttgtt ataactccta tgaaacaaca tcaaagatat tttccagtac
    18481 ttaaagaagg aaaactgtta cctaacttta tagctgttag aaatggtgat tcttacagaa
    18541 tagacaatgt taaagctgga aatgaaaaag ttttagaagc aagacttgca gatgctttat
    18601 ttttctatag agaagatact aagagaaact tagaaagcta catagaaaaa ttaaaaactg
    18661 ttgtattcca agtaaaatta ggaactattt acgacaaaac tttaagactt gaaaaattaa
    18721 gtgctgatat acttgatagt ttaggtttag tagatgaaaa ggcagatact ataagggctg
    18781 ctaaattatc taaggcagat ttagttactg gaatggtagg agaatttgat gaattacaag
    18841 gttttatggg aaaagaatac gcaaaagttg gaggagaaaa tgatgttgta agtgaagcta
    18901 tttttgaaca ttatcttcca agatttgcag gagatatact tcctaagact aatgcagggg
    18961 ttgccttatc aatagctgat aagttggatt caatagcagg tttctttgct ataggaatac
    19021 aaccaacagg ttctcaagac ccatatgctt taagaagaca agctttaggt atacttagta
    19081 tacttatgga cagaaaagtt gctgtggata taaaaatact tattgaacat gctttagaca
    19141 attattctga attagatttt aataaagaag aagttgttga gcaaataatg aatttcttca
    19201 atgagagagt aaaaaatcta tttaaagatt taggaataag atatgatgtt gttgatgctg
    19261 ttttaagttc tgatataaat gatgtttctg atatgcatat gagagcttta gagcttaata
    19321 attggctaga aaaagatgaa ttagtggaga tgcttactgc atttaataga gtttctactc
    19381 tagctcaaaa agcagaaagt gataaaattg atgaatcttt attaaaagaa gatgctgaaa
    19441 ttaaacttta caatgaattt aaacaagtaa aggtaaaagt taatgaatta ttaaaggata
    19501 agaaatatgg tattgcttta gatgaatttg catctttaag accagtagtt gataatttat
    19561 ttgatacagt tatggtcatg gataatgatg aagctattaa gaacaataga cttgctttac
    19621 ttaaacaagt atatgatact atgctaggaa tttgtgattt atctaaaatt gtttataaat
    19681 aaaatttaaa ctatttataa gaaataaggc tgtctcaaaa tagagatagt cttatttttc
    19741 attgttgaga tgatgaaaat atatttgatt tcatagaaaa agagtgtctc ataaactaaa
    19801 aagttcattt tgagacaccc atgttttttg taagtaaata taataaatta tattttatac
    19861 ttatcagtta ttttgtttaa aatattatca tttgaattta cccattcata aaaattgcct
    19921 gaagatgcgt ccgatatttt ataaagcatt tcaggaatat cactagcttt aaaatctcca
    19981 agtgattttc ccaatctagt cttacctatt tcaaaactgc ctccaacaaa taattcaaat
    20041 gcatctgtag atataccatc aacattcttc tttttaccac ataaacctat gctaccaact
    20101 tgatgaacac cacatgaatt taaacatcct gatatatata acttaggtgc tttatttaaa
    20161 atttcttcat tattttgtaa tctaaaataa tctatgattt catgtaacat tttctgacta
    20221 ttttgtatgc ccatttgaca aattggaaca cctatgcaag atatactttg ttctaattga
    20281 gaattacaac taatagtttt acttatttct aatattcttt ttgcttcatt tccatttaaa
    20341 tttaaaatat ataatccctc tgtcattcct agtctaatca taggattttc aacattatct
    20401 aattctttaa gtaaagtact taaatccttt gttaacaaaa tacctccaac tggatgtata
    20461 tatacagtgt ataaaccatc ctgtttttga ggtattagtc tagaatcttg tatatctatt
    20521 ttaactccag gtttagagta atctatttct tcaatattga gttcaagact accattttct
    20581 ttttctaatt tatagtattc tttaaatttt tctaagaata gttcttcacc aagtgattcg
    20641 accatatatc taaccctagc tctgttttta ttttcataat ttccataatc cataaagaat
    20701 ttaatcatac cttcaacaca atataaggcg tctttcggct ttataagttc atcaagttct
    20761 aaagcaactt tgggttgttt acctagacct ccacctaaga aaactctgaa atatggttca
    20821 tcatttttca aagtagcaac aaatcctaag tcttgtatac tacaatgagc agtatctgtg
    20881 taacaattag aataagaaac ttttaatttt ctaggtaaat ggtatgtagt agcatttttg
    20941 ataaaatatt tatcagtagc aactgcatat ggagtaacat caaagacttc actagggtca
    21001 actccagata atggagataa tccgacattt ctaggataat taccaccacc acctctagtg
    21061 aatatatcat tttttattcc ttcttccatg atatcaacaa ttgaatttat agataaatcg
    21121 tgtaattgta tagcttgtct agttgttaag tgtatcttat ctaagttgtg tttaatagct
    21181 atttcataga ttttattaag ttgatactga gataaaactc cagcagatac tctaagtctt
    21241 atcataaaag attttttgtc tctttgagca taaacaccat atccaccaga aaatccctta
    21301 tattgcattt tatttatttc tccatttaaa aattttgaag aatattcttt aaaattaggt
    21361 agttcattta acaatatctt tttaaaatta tccattattt cacctcattg ttagtcttgt
    21421 ataaaaat
//
LOCUS       DACULQ010000052        21253 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.052, whole genome shotgun sequence.
ACCESSION   DACULQ010000052 DACULQ010000000
VERSION     DACULQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.052"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            129..803
                     /locus_tag="JA154_07950"
     CDS             129..803
                     /locus_tag="JA154_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAT7995798.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(925..2319)
                     /locus_tag="JA154_07955"
     CDS             complement(925..2319)
                     /locus_tag="JA154_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAT7995799.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFTFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(2397..3743)
                     /locus_tag="JA154_07960"
     CDS             complement(2397..3743)
                     /locus_tag="JA154_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT7995800.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      3993..4095
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            4189..5565
                     /locus_tag="JA154_07965"
     CDS             4189..5565
                     /locus_tag="JA154_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAT7995801.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(5679..6824)
                     /locus_tag="JA154_07970"
     CDS             complement(5679..6824)
                     /locus_tag="JA154_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAT7995802.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            7077..7784
                     /locus_tag="JA154_07975"
     CDS             7077..7784
                     /locus_tag="JA154_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7995803.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            7831..9426
                     /locus_tag="JA154_07980"
     CDS             7831..9426
                     /locus_tag="JA154_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7995804.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMLIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            9529..10611
                     /locus_tag="JA154_07985"
     CDS             9529..10611
                     /locus_tag="JA154_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAT7995805.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            10760..10921
                     /locus_tag="JA154_07990"
     CDS             10760..10921
                     /locus_tag="JA154_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HAT7995806.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            11262..12530
                     /locus_tag="JA154_07995"
     CDS             11262..12530
                     /locus_tag="JA154_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAT7995807.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            12725..13513
                     /locus_tag="JA154_08000"
     CDS             12725..13513
                     /locus_tag="JA154_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAT7995808.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            13513..13962
                     /locus_tag="JA154_08005"
     CDS             13513..13962
                     /locus_tag="JA154_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HAT7995809.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            13966..16230
                     /locus_tag="JA154_08010"
     CDS             13966..16230
                     /locus_tag="JA154_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidase"
                     /protein_id="HAT7995810.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVK
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGRMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            16370..16681
                     /locus_tag="JA154_08015"
     CDS             16370..16681
                     /locus_tag="JA154_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995811.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            16967..17467
                     /locus_tag="JA154_08020"
     CDS             16967..17467
                     /locus_tag="JA154_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAT7995812.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            17524..18510
                     /locus_tag="JA154_08025"
     CDS             17524..18510
                     /locus_tag="JA154_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7995813.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            18560..19282
                     /locus_tag="JA154_08030"
     CDS             18560..19282
                     /locus_tag="JA154_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HAT7995814.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            19311..21245
                     /locus_tag="JA154_08035"
     CDS             19311..21245
                     /locus_tag="JA154_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAT7995815.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKQEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
BASE COUNT         8130 a         2274 c         3517 g         7332 t
ORIGIN      
        1 aaaaattata aaactaagaa ggaaaagtaa aacaataata aaaaataaaa aacaataagc
       61 aaaatgttga aaataatacc aaatcaatat ataataagaa taaagaaaga ttaaaggggg
      121 atattttaat gcaactaatc ttagatacag gtaatgtgga ggagattaag gagttatgta
      181 cttgtttacc aatagatggt gtaactacaa atccaagtat tgtttcaaaa gaaaaaaaga
      241 attttaagca acttataaat gaaattggtg agattatagg tgaggatatg cctatacatg
      301 ctcaagtgtt aagtacaaag tatgaagaaa tattagaaga agcattatat ataagtagtt
      361 tgagaaaaaa tatatatgta aaaatacctg taacacaaga tggtttaaga gctataaaag
      421 accttcatag aaaaggtgta aaaataactg ctactgcaat attcactgct catcaaggtt
      481 ttttagctgc aaaggctgga gcaaattata ttgcacctta tgtaaatagg cttgataata
      541 tatctggtga tggagtagct atggtttcag aacttataaa aataatagat acttataaaa
      601 tggatacaaa agtacttgca gccagtttta aaaatgcaca acaagttata gaattaatgc
      661 aacatggggt gcatagtgtt acagtaccat atgatatttg taaatctatg atgaatcatc
      721 cattaactga ttggagtgta gataagttta tagaggattg ggagaatact tttggaaaag
      781 gaagtaaaac aaataatatt tagtattatg gtaaaagagt agttttgtcc aattttacaa
      841 gaacttatat aaaaagatga aaatatgaat tttcttttta ataatttcgt tattatctta
      901 agttaattca tattttcatc ttttttagat ttctagtcct ataaatttcc accaagggaa
      961 tgttatacat attattataa agtaggttaa aggaatcaaa gttaaaccgt atttgaaaat
     1021 aaccttgcta ttatacagat tttcaccatg accaaccatt attgtagcat gatgaaatgg
     1081 taatatgtac tgtatgctta ctataatgta gacaaataga cataaagtaa cactattttc
     1141 ctttagtata tgtaaacttc caagtaatgg tattactaca gatgatgtag tgacagcact
     1201 tccaagacat atattcaaga ttattgtgag agttatcaaa aacaatattg aaataatttc
     1261 tgaagagttt ggtataaact ttatcaaata atttcccatt atatctacaa ctccagagta
     1321 ctttaacact cctccaattg aaaatgctgc tataaaaaaa ataaggacct tccaatttat
     1381 ttcttttaaa gtactaaatc caactatttt tcttagtaat aaaagaagta cacaaattga
     1441 cattataatt aatgtgttta tgttatgtat gaactgagtt aaaaacataa aaaacattat
     1501 tcctaggcat aaaaatacgt gtttttgttc tttgtttagc aaattaaatt ctttttttaa
     1561 attagaatat gaaaaatgaa gtttattacc ctcaagattt tcattagaaa taaattttat
     1621 tgaatgcaat tcttttttaa agacaaaggt aaataaaaaa taagttataa aacaagtaat
     1681 tatagttgga acagacatat aaaaagccca ttgaccccaa tttatattac aatttccaag
     1741 ttctagtaca acataattta gaagagtgtc tccatttaca aaaaacattg atgtaaaggt
     1801 agatgctgta aatatactaa ataaaagtat agattttgaa tcttcagtta tctgttgttc
     1861 ttttaaaaac tccttgtaaa aagcagatac aagaataacc cttggaaatg gttgtgggat
     1921 aaaaaatatt aaaagtatac ctaaaatata cgataaaaga atcatttttt taggggtatt
     1981 tacagtattt aaaattaact tctcagaaat aatttttgcg acacctgttt ttgtaactgc
     2041 attagtaatt atgtatgcaa gaattattat gtaaaaattt tctgtaaata aaaactgtaa
     2101 aataaccttt attggtgcaa tgttaaatat aaacgctaga attacaaaaa acaatgcaac
     2161 aaaagttttt tcaactgagt ctacagccca tatcaagata gttaaaaaag ataagcatat
     2221 agaaaatttt atgttgaaac tgtaattaag agagaagtag agaattatgg tagctataac
     2281 agtcatataa aattgttttg gcttaagaat aatacccatt tttatattca cctccataat
     2341 ttattatatc ataatggagg tgaatacaat gaatgtgaat acattttata aaaagattat
     2401 tcattgattc tatgatttac aatagcttta gttttccaat gtcctttctt atagtagata
     2461 actgtaacaa taagacctaa agtccaacca aaaggaatac ccatccaaat tccgtctgtt
     2521 ccaaaaaatt tagaaagcat ataagaagct ggaagtcttc caagccataa agatactatt
     2581 gagcttataa gtgcaaacat ggaatcacca gcacctttca ttgcacttga aagtacaaaa
     2641 gaagttccaa taaataagaa gaatggtcct attataaata agtaagaaga gccaatttta
     2701 atgacatctt tatcagtatt gaatagagca attagatttt ccctaaatag caaaattaaa
     2761 gcaataactg taactgctaa aactaaggtc atttttatag attctcgaat gcctttttta
     2821 actctttcat tttctccagc acctatgttt tgagcaacaa aagtagaaac agcagaacca
     2881 agattcataa taggtaagct tataaaattt tctatcctaa gaccagcacc aaaagctgcc
     2941 atagcagatg taccataact attaactaaa aattgaagag ccatatttcc aattgaaaac
     3001 aacatttgtt gaactccact tggaagtcct aatctaagtc catctttaaa tattttgtta
     3061 tcatatacaa ttcccttgat tttaaattta agcacttcat gttttttatt taaataaatt
     3121 atacttatta taaatgatac tgcctgagcc attatagtgg caagtgcaac gccctcaaca
     3181 cccattctta gcactacaat aaaagttaag tctaaaacta tatttagaat tgtggctatt
     3241 atcagaaaat aaagtggagt tactgaatct ccaagtcctc taagtattgc acttactgaa
     3301 ttatatccag cagaaaataa tattccacta aatattatag taagatacaa ttttgattga
     3361 gcaaaaacgg attctggtgt attcataagc ttaagcattg ggccaccaaa tactattcca
     3421 agaatactta ttaatataga accaataaat aaaagtatat atgtagtatc aatagtagct
     3481 tttacctttt ttaaatctcc tgagccataa aattgtgaca ctaaaataga aaatccaagg
     3541 ctaacaccca taagcgctgc caccaataaa aacattatag ggttagctcc agagactgcc
     3601 gccattgctt cttttccaat aaatctacca actattatac tgtcagctgt attgtatagt
     3661 tgttgaaata aactaccaat aagcataggc atggcaaaaa agaatatgga ctttccctca
     3721 tgacctgtag ttaaatcttt caccttattc ctccaaataa aaaaattatt aatttattat
     3781 agtataatct taacataaac tttatatcca aactataaat aaattgtatc aaaatatcat
     3841 atattttttt aaaatgtaaa gaatataatt ataatttgaa tttaataaat caatatttaa
     3901 tataatcgaa tattaataca aaatcaatac aatatatacg tatatattga caaaattaat
     3961 tatataaatt aaaataatat tcgtaaagaa aaattcatgt tttgtactgt ataacatata
     4021 attttgacaa tatgggtcat aagtttctac cggaataccg taaatattct gactatgtat
     4081 actattattt agttaattaa atatttaatt aactgaatta tgaaggatac ggctcagctt
     4141 atcaactact gtgtcgtttt tttgtaaaat ttaataggag gtgttgttat gcaaacaaca
     4201 aacagtagta ctatgcagaa actttttcca attttgacta ataaaaatgt cgacatgaag
     4261 aaggaaataa ttgcaggagt aactactttt ttaactatgg cttatataat agctgtaaat
     4321 ccaaatattt tatctgagac aggcatgcct gcaggtgcac ttgttacagg tacttgttta
     4381 gcagcagcat ttggatgtat tttaatgggt gtagttgcta accttccttt tgctttagca
     4441 tcaggtatgg gtcttaatgc attttttgca tatacagtag ttttacaaat gggtgttcct
     4501 tgggaagtag ctcttacagc agtatttgta gaaggtatta tttttatagt attatcagta
     4561 agtggagttc gtgaggctgt tgtaaatgcg ataccaaaaa atatgaaatt agctgttaca
     4621 ggtggtatag gaatatttat tgcacttata ggtctagtta acagtggaat tgttattgga
     4681 gaccaagcca ctcttatcaa aatgggtaga tttacaccag cagtaataat aacttgtgta
     4741 ggattaataa ttattgctgt attagataaa aaaagagtta aaggttctat actttttgga
     4801 atagtagtga gttcattact tgcttgggca tttgctttta taaatccaga gtatgcacaa
     4861 aaattaggta tatatttacc tggtggatta tttaaattcg aaagtttagc accaatagca
     4921 ggtaaaatag atttaggata tgtactacat ccaacaaata taggtggttt tttagtagta
     4981 gtttgtacat ttttatttgt tgattttttt gatacagtag gtacattagt tggtgtttgt
     5041 tctaaagcaa acatgttaga tgaaaaaggg aatgttccta atgtaggtag agccttactt
     5101 acagatgcgg ttgcaactac agttggtgca ggacttggag tatctacagt tacaacttat
     5161 gttgagagtt caacaggagt tatcgcaggt ggaagaactg gatggacagc aataacagtt
     5221 ggaattttat tcttagctgc aatgtttttc tctcctgtat ttatagcaat tccatcatgt
     5281 gctacagcac cagctttaat atatgttgga tatttaatgc ttggtacagt taaggatata
     5341 gagtttgata atataactga aggtgttcca gcttttgtta ctattgcttg tatggcatta
     5401 acatatagta taggtgatgg tttaacttta ggtatattaa cttatgtttt cgtaaatata
     5461 ttttataata tatttggagc taaaaaggca gaagacaaaa aacatgtttc tgtagtaatg
     5521 attgttctgg caatattatt tataataaaa ctttggctac tataataggt attatttaaa
     5581 attttaattt aatataaaat aaagggctgt aatttattat caattgtgat gataaactac
     5641 agctcttttg tatttgttgt atttttgtat ttattgtatt atttaataat gtctattagt
     5701 tctccatttt gtaaagtttt aggtgtaatt actatattga actctttttg caaatctttt
     5761 ataatatcta attctttttt tgttattatt gagattgtgt taccacttcg atttcctcta
     5821 gcagttcgac cacatctatg caggtattca tttttacttt ttggaaaatc caagttaaaa
     5881 acatgactta catccacaat atctagtcca cgagcagata aatcagttgt aattaaaatc
     5941 tttgctttac caagtttaaa tttatttatt gcattctttc tatcttcttt ttccatattt
     6001 ccaaaaattc ctattgcttt gtaattatga taatttagtt ttgatactag gacttctata
     6061 tttttttcat tatttacaaa tacaatagct ttttttggat ttgtagctgc taatgccttt
     6121 ctaagaaaag taaatttatc ccttatctca cctaataaat atgagtgatt gatgttagga
     6181 tttatttgtg atttttcctt tgtttttatt atttcaaact ccttcataag tttatcacaa
     6241 atttttatag tgctatcagt aagacttgca gagcatccta taatttgtct atctcttaac
     6301 gttgttctta ttatgtcttc tatacaagtt atattttttc cattcaatag attgtccact
     6361 tcatctaaga caatagtttt tatattatgt gattttagtt tcttttgttt tatcaagtct
     6421 aaaactcttc cacaagagcc aattactatg tgtggtttta ctgcttttat attttttatt
     6481 tgtttttgta tattgacctc cccaattaaa gctaaagaag taacggagag ttctgcattt
     6541 ttagccaata attcaacctg gtttgtaatc tgcattacta attcatgcgt aggtgcaagt
     6601 ataagagctt gtgtttctct ttttgatgta tcaatttttt caaatatagg taaaaggtac
     6661 gctagtgttt ttcctgttcc agtctgtgaa ttaatcaata aatccttatt ttgaattata
     6721 ttccctataa caagtgactg aacttctgtt ggagaagtta tgtcttgttt tttgagtcca
     6781 tctattagag tggaacttat ttttaattgt tcaaatgtat tcatatttta ctccttatat
     6841 tttaataatt atagtcaact attttttatt atacattgtt tattcaaatc gaactatata
     6901 ttcttttata attatgtgta atttgaaagt aaatatcaat tatatttata cctttatatt
     6961 tctgatacct ttgcatttta tagatttcta aaataaacaa ataaaagttg taattgtttg
     7021 atatttgtta tatattgtat ataacaaata tcaaaactat aatatggagg gaaattatgt
     7081 taaaagtaat caatgtatct aaatcatata aaaataaaaa agtagtaagt agtgtctcat
     7141 ttgatgtaaa tgatggagag ctttttgggt ttataggaca taatggagca ggtaaaacta
     7201 ctactattaa agcaattgtt ggaatacacg attttgaaga gggagaagta ctaataaatt
     7261 ctaaatctat aaaaaaacag cctgttgaat gtaaaaagga aatggcatat atacctgata
     7321 atccagattt atacgagtca ttaactggta tacaatacct taattttata gctgacatat
     7381 ttgaagttgc caaagataaa agagaagagt taataaaata ttattcaaat aagtttgaac
     7441 tcaataaagc attaggagat ttaatatctt catattcgca tggtatgaaa caaaaattag
     7501 ctataatttc ggctttgata cacagtccaa aaatcttaat tcttgatgaa ccttttgtag
     7561 gtcttgaccc aaaagcttcc tttatattga aggaaattat gagggagttt tgtgacaaag
     7621 gtggttgtat atttttctca acacacgttt tagaagttgc tgaaaaaatt tgtgataaga
     7681 ttgccataat aaaagatgga aatataattg cttatggaac tacagaagaa gttaagggaa
     7741 gtaattcact tgaaagtatc tttatggaat taatagagaa ataaaatgtt ttttatacaa
     7801 tatagtgtct aaagtgagga atgataaaat atgagcaatc taagtatatt aataaaaact
     7861 aatataataa atgaatttaa attaaatgcg ttaaaaaaag ctgatggtag agaaaaacaa
     7921 aaaataatgg gaatgatgct ttcagttgtt ataatagcgg gtctattaat tttttatgta
     7981 acaagcttaa gtctctcttt agttgatatc ttaaagcaaa taaatcaact ggaaatgcta
     8041 ctgatatttg gttttggatt atcaacatta acaactattg ttacatcatt atataaaacg
     8101 tcatcatatc tatttcaagc aaaagattta gaccttttga ctagtttacc tataaaagaa
     8161 tctactgtac tagcaagtaa gatattaatg ttggttggag caaactattt attttcttca
     8221 atatgtatgc ttatacctgc agtggtttac tttttaaatt ctgatgtaag tataacttat
     8281 ttcccttatt tgatattgct atttttagct ttaccattat taccgatagt tgcttcatca
     8341 ataatttttc tatttatagg gcagatatca tcaagaataa aatacaaaaa tttagtatta
     8401 atcataggta gcattatatt gatgttgctt tataccctac ttatgtcaag acttaattct
     8461 atgtcagttg aaatgctaaa gaatagtgtg tctatttcag agacgattag aaaagtatat
     8521 cctcctatat attattttgt tgatgctctt aaaacaggaa gtatagtttc atttttagca
     8581 tttatagcca tctcaataat accatttttt atgtttgtaa ctctgtttgc aaaaaaattt
     8641 aaagatataa atgctaagat gagtgaagga tataagtcaa aaaattatga atttaagggt
     8701 caaaaaactt caagaccttc aaaagcttta ttaaaaaaag aatttaagag atatacttct
     8761 tcgtttatat actttttaaa ttcatctgca ggtttgttat ttctaatagt aggtacaatt
     8821 ttaatattat tatttggagc agataaagtg ggtgagttac tgaaattgaa tatagatttt
     8881 aatcttataa aaatacaatt attgggagta ttcttcttta taatagtaat gaattgtaca
     8941 acatactgtt caatttctct tgaaggaaaa aacttatgga tactgaaatc ttctccaata
     9001 gatgaaagag aaatattctg gtcaaaaata ttgatgaatt ttttattgaa tgcaccacta
     9061 tctattttat gtctaatatt agtatctata agattagact ttgaaattca gtttattatt
     9121 gtatcaatct gtttgattgt aagtttttca atgtttgtag cattgatggg attattatca
     9181 aatttattat acccaaacct tgattggaaa aatgaggtgg ctgtagtaaa aagaagtgca
     9241 agtatgatag ttacaatgtt agtatcatta gcttatgtaa ttatacttgg aggtatatat
     9301 gtattacttg gaatttcgtt tttaaatata tatattattt tggcaagtat acttactttg
     9361 atattagatt ttatgatatg gagaattatt tctactaaag gagtaagaat atttaaaaat
     9421 ttataggtgt atttatgtca aataaagact tatctttttt agtcttgcaa ataataggtt
     9481 aagtagtata ctatgtatag tgatatgaaa aaatttagga gaaaataaat gagaaaatta
     9541 tccaaaagag aaaaagattt tattcaatat ggatttttat tatttttgat atgtattact
     9601 acttatttag tattaactac tttagatata aaactaatac ctaaaattat aaggctagta
     9661 aataagactt atatatttct tggcatattg acaattatag tatatatgat aattgaatct
     9721 attgtaactc atttgataat aaatgcagtt caaaaaacaa aagtaaaatt tattggtatt
     9781 aaaatggcta caatgggatt ttactataac ttagttactc catttgcatc tgggagtcaa
     9841 ccaatgcaaa tatatgcact tacaaaatat gatgtaagcc ttagtaaatc agtggctata
     9901 gttactaata aaactgtagt atttcaaagt acagttactg tattttgtgg aattttaatt
     9961 ttgctaaata agtcattact gatggagcaa gtacattctg tgaaagtctt gataactttt
    10021 ggaatgatta tgaatgtaag tatgttgtta ggtggactac taatagtatt tagtccaaaa
    10081 caagtaaaaa tgatagcaga agttttgatt aattttttat ctaaatttaa aacatttaaa
    10141 tttttagatt caaaacgtga tagaataaat cattatattg atgattataa ttattctata
    10201 aaaatattta ttaaaaatgt aagaatctta gttttaagta tattacttac tgtgttacaa
    10261 ttaatcgcat atttcagtat agtttattgt gtgtataaag cctcaaattt aagaggagtt
    10321 tcatatgtac atataatgac attacaggtc tttttatata tggctatatc accaattcca
    10381 acaccaggaa atgttggagc taatgaggtt acatttttta ctatgttctc caatataatt
    10441 ccaaaagaat tattggggta ctctgtactc ttatatagtg ggtttgtata ctattttata
    10501 ttaatagttt caggtttatt tacatttgga acgcattata cattaaattc ttggaaaaag
    10561 gaaaataaag taaattctaa tttagataag gttattgaaa aattaaatta ataaaataat
    10621 tagggaaatt ctatatataa atatggtgtt gaataagcta tattatattg agatatttcc
    10681 cttttttgat taaaattata gaaatttgaa ataataagta ataaacagaa aaagtgatat
    10741 tgttttgaaa ggagaaacta tgaaacaaga taaacaaaat gaaaaacaaa gtgagatttc
    10801 taagataaaa caaagcaatg ataataaact tgaaatagct caagaattac atgtaagtaa
    10861 aaatataggg aatatgcttg gatatagaat agataagaaa aaaatgaata gaccaagtta
    10921 atgaaaatgt tgaaaatggt acattttaaa aaatatatat aatgaaatgt tttattgtta
    10981 aatttaaact tattttaatg ggggaaaaag tcgaaaaata tctaaaaaga tatggatata
    11041 aaaaatatta atatatatat actataaact tgacaaaatc aaccttttaa taactagcaa
    11101 aaaatagcaa aattatataa gcgttattta attgtcaaaa atgaaagcta aattgcatgt
    11161 cctaaaaaaa atgattgata acatttattt gtgtgctata attatatagt aaaacaataa
    11221 gtatattgca taatgtatgc aaatcgggag gagagacgga catggaagca tctatacaca
    11281 caattgccag caataattta ttgataattt ttgctattgt aagtataact ggaataattt
    11341 gtagtaagct tagtgaaata ataaaggttc cagatgtagt gttatattta cttgtaggta
    11401 ttttaatagg accatcattt ttaaaattta tagatattcg tggatttcaa atagaaaatc
    11461 aactaatact tacatttggt tctgcattta ttttatattt aggtggaaaa gaaataagtt
    11521 taaaagtatt gagaaatgtg aagatatctg tattcttatt agctacttta ggagttgtga
    11581 tatctgcttt tataatgcaa caagtaatag gatttacttt tgggataagt gcaatgactg
    11641 ctttactagc aggaactata atagcatcaa cagacccagc cactcttgta cctatattta
    11701 atcaggtaaa gataaaggat agggtaaaac aaactgtaat aagtgaatca gcttttaatg
    11761 atgcaacagg agctatacta acatcagcag tgttggctgt aattttgtca aataaatttt
    11821 cattaggtga aaatatttat gaactgggga caatggttat tgtcggagtt ttagttggtt
    11881 taattacagg aatactgctt ttgaaattag ttaatgataa accttatgga atatttaaag
    11941 attttgcacc aataatatct gttatttcag ttgtagttgc atatgaatta tctacaaaat
    12001 taggtggaag tggatatatg tcttgcttta ttgtaggtat aataactggt aacaagaaaa
    12061 actttaaaat ttggttaagt caaaaatctt atgatgcaga tttttatgtt gctgaaactt
    12121 taggtactat ttgtcgtatg gcaatattta taatattggg tagccaagtt aatttggctc
    12181 agttaagtaa atattttatg ccatctgtaa ttacagtatt agtattaatg tttatagcaa
    12241 gaccactttg cgttttagtt tgtacacttg tagacaggga agcaaaatgg agtaaaagtg
    12301 aaatattatt tatgatgtgg gtaagggaaa caggcgtaat accagcagca ctttgtggta
    12361 ttatttcagc aatgaaagtt cctggatacg aagtgatttc atctgtagta tttatgacta
    12421 tactaattac attagtaata caaggaagta ctacaaaatt agtagctaaa aaacttggat
    12481 tattagaaga agaagtgata aatataagtg aaaaagtatc aactttttaa agttttgctt
    12541 taaaatagta cctgttaatg gctattttaa tattgtctaa tgatgattat tgaaatagtc
    12601 atttttttat gtataagtaa tttacagaat ttacatatta gacatattta ttatcaaaat
    12661 ttgttgaagc tatgatataa ttagttgtat ttaaaataag aatagcaaat aagggaggaa
    12721 atttatgttt acggttatgg atattgtaca gccagataca gtagaagaag cgtactcaat
    12781 tttaaataaa agaaaaacta accaagtaat tggtggaagt gcttttttaa gaatggggaa
    12841 aaaaagaata ggaacaggaa tagaattatc taaattaaat ttagattata taaaagagta
    12901 tgaagattac gttgagattg gttctatgac tacttttaga acattagaaa caagttccat
    12961 aataaaaaat aattttggaa gaataataga agattcagtt aaaaatataa taggagttca
    13021 gtttagaaat gtagtaacag ttggtgcaac agtattttct aaatatggtt tttctgactt
    13081 gatagtagca ttactttcat tagatacaga agtagaatta tataatattg ggagaattag
    13141 tcttgaagag tttctaaata gagattatga gaaagattta cttattaaga tatacataaa
    13201 gaagacaaat aagaatgctt cttataaatc acttagaaat gcaaagagtg attatccaat
    13261 tctaaatgta tctgtatcaa aacatatgga acaatttaag ttatgtgtag gagcaagacc
    13321 gcagaaagct acgattgcaa aacaagctag tgaatttcta tctaacaatg aaatcaatga
    13381 aattaatata gataaagcag tagaaatagc atcagaagaa ctaacttttg gttctaacat
    13441 gagagcatct agggagtata gaaaagctat gagtaaagta cttttaaaaa gagctataat
    13501 ggaggttatt taatgttagt aaagattaat ataaatggga agaaaaaaag tgttgatata
    13561 gaaccagaag aatatctggt tgatacttta agaaaagttg gtaatttaag tgtgaaaaga
    13621 ggttgtgaca caggctgttg tggtttatgt acagttttaa ttgacaagaa acctacatta
    13681 tcctgtgcaa cactgacatt gagagcttta gataaagaaa taactacaat tgaaggttta
    13741 gaaaatgaag ttaaagaatt tagtgaagtt ttggtaaaag aaggagctga gcaatgtggt
    13801 ttctgttctc ctggctttat acttactgta cttgcaatga aggatgagct tgaaaatcct
    13861 acagatgatg aaataaagca ttatcttaca ggaaatctat gtagatgtac agggtatatg
    13921 ggacagttaa gagctatcaa aacttatctg gaggtaaaat agaacatgaa tatagttgga
    13981 aaaggaatca agaaaataga tggaatgagt atcacaacag gaaagcctgt atatacagat
    14041 gatttatcag caaaagatgc tttggtggta aagatactta gaagtcctca cgcttatgca
    14101 aaaataaaag atatagatac atcaaaggca gaaatggttg atggtgttga atgtgtttta
    14161 acatataaag atgtaccaaa atctcgattt acacttgctg gtcaaacata tccagagcca
    14221 tctccatatg atagattaat tcttgaagat gttgtaagat atgtaggtga cgaagtagct
    14281 atagtggcag ctatagatga aaaaactgca attaaagcaa tgaatcttat caaagtaaag
    14341 tatgaagtac ttacacctgt tttggatttt gaagaggcaa taggaaataa aactatggta
    14401 catgatgaag atagacattg taactttgat ataggtatgc aaagagaaaa aaatatagta
    14461 tcccatcatg attatacaaa aggtgatgta gaaaaagtat taagtaattg tgatgtagtg
    14521 attgaagaaa cttattatac tcaagctcaa gcacatgcaa tgatggagac gtatagggca
    14581 tacaattatt tagaccatat gggaagattg gttgtagtaa gttcaactca aataccattt
    14641 catgtaaaaa gacatttatc tcgtgcacta gaaataccag ctagcaaaat tagggttata
    14701 aaacctagaa ttggtggcgg atttggtggt aaacaaactg catgtgttga gattttttct
    14761 gcaattgtta ctttaaaaac agggaaacca gctaaaataa tttatgatag aaaagaaact
    14821 tttaattgtt caacaagtag acatgcaatg agattaaaag ttagactagg tgctacaaat
    14881 gatggcataa taaaggcaat agatataaaa gcactttctg atacaggagc atatggagag
    14941 catgcttcta caactattgg tttggttgga gaaaaaactt taccaatgta taataaagtt
    15001 gaagcaacaa gatttattgc agatgtggta tatactaata agatgccagc aggagcactt
    15061 agagggtatg gagcatcaca aggatgtttt gctgttgaat caactataaa tatgcttgct
    15121 aataaattaa aaatggaccc aacagattta aggttaaaaa atgtggtaac agaaggtgaa
    15181 actacatttg catatggtaa aactttaaat agtgtcgatt taataaaatg tataaataaa
    15241 ggcaaagaac ttattggatg gaatgaaaaa tatccttgta aacatcttga taatggaaaa
    15301 attagaagtg tgggtatggc aattactatg caaggttctg gtatacaagg tcttgataag
    15361 tcttcagctg aaataaaatt gaatgactgt ggagattata ctcttatgat aggttctact
    15421 gatatgggta caggaagtga tacgatttta gctcaaatgg ctgctgaggt tcttgataca
    15481 agcattgaaa atataacagt tatatcagct gatactgact tagttccata tgacccaggt
    15541 tcatatgctt caagtactac ttatgtaaca ggtatggcag tagttaaggc agctcaagac
    15601 ttaagagaaa aaataatttc tgctggagca actaaactag gtgttgagaa ggatgaagta
    15661 aaatttgatg gtataaaatt aactagtaaa aaaggtagca ctttattatt cgatatagca
    15721 gttgattcta caattggtcc agatactaat caattaatag gtcttgcttc tcatggtagc
    15781 ccagtatctc ctcctccttt tatagcaggt tttgcagaaa ctgaaattga cacagaaact
    15841 ggaaaagttg atttagtaaa ttatgtagct gtagtagatt gtggaacggt tataaataaa
    15901 aatctagcta agatacaagt tgaaggtgga attgttcaag ggattggaat ggcattattt
    15961 gaagaagtta gatacacaga aaaaggaaga atggacacaa atacatttat gcaatacaag
    16021 attccagcta gatgtgatat tggaaatgtt aaagtagatt ttgttgaaac ttatgaacca
    16081 actggaccat ttggtgctaa atctgttgga gaagttgtta taaatacacc tttaccagca
    16141 atacaagaag ctgtgtttaa tggaattggt gtaagaataa atacactccc tattacacct
    16201 gaaaaagttt ttatggaaat gaataaataa ttatttatga aaatttagac tagttttaca
    16261 tgttaaacaa ccagttatgt catacctgtt gaaagatatt taataagtag ttattataat
    16321 attatgaatt ttacttataa aatacaaaat taaataaggg gatgatgttt tggctggtaa
    16381 tctaaataat atgagagcag taaataattt tagaggagat aagaacattt tagaatgttt
    16441 agtcagcttt gagggtcgtt caataagtca gagaaaagta agggtatttt ttaaagaaaa
    16501 acaaaatcaa atagaaattg attttgcaga agaggaaatt tctaaattgg ttgaaaatgt
    16561 tgttttaaat acatcatatc aagaaatgtt atatgatgaa atagagaaac aactggaaat
    16621 tgattgtata ggtacttgga tgatattatc taaattaaaa gatggtagta gagttcactg
    16681 attttaaaat aaattattag agcctaattt taaggctctt ttttgctgtt tttaattaac
    16741 aatagaaaac ttatctttag tttgtatata attttaatat catatattct atatatataa
    16801 aattgaattt gtatgtatta tattgtataa tgtaaaatga attggaaaaa tagttcgata
    16861 aaataatttg tttttataaa attactgttt aagtttatag attttgtata aaataaagtt
    16921 atgttattaa tttttttaga aataaaactt ttactaggag acagatatga gaaaaaatag
    16981 tattaatata gcactaactg cagcaattac agcaatctat gcagttttaa cattatcatt
    17041 gggatttata agttatggac caatacaatt tagggtagca gagattatga tgcttttggc
    17101 atttttagat aaaggatata tagtaggtct tacattaggt tgttttttag caaatgtaat
    17161 aggaccatat ggtgtacctg atataatttt tggaacatta gccacattta taagtgcaag
    17221 tatgatttat attactagaa agttaggtaa acagaataaa agtactttag ttatagcttc
    17281 tttatggcca actgttataa attcgattgt ggttggtcta atgttaaatg tattctttgg
    17341 gcttccatta attctatcta tgatacaagt aggttttgga gaatttgtag tagtgacaat
    17401 tgtaggagta ccatttttct gctttttaat gaaaaaatat aaaaatgtca ttaatattaa
    17461 attttaaata gtgtatagta atttaattaa ttgagagtat tattagtgaa aagaggtgtt
    17521 tatttgtcga aggtaaaaag attgactgat gtttggatat atattaatga taagcaaaag
    17581 tttacaataa aagagttgtc agaggaattt aatctttcag ctaaaacaat acaacgatat
    17641 ttaaaagaat taaataaaat gggacttcca attcaagcag aacaaggtag aaatggaggt
    17701 tatagggtgc taaataatag ctatatacct cctgttatat ttacagaaaa agaagttatg
    17761 attatactat ttgcacttaa gtcacttcag atttataatt ataagttaat ggaaatagaa
    17821 attaattcta taatgagaaa agtaagttat gaacgtaaaa gcagtataaa agtaggtata
    17881 gaaaatatta aaagatacat tggctttgta gctaatgata ctaatgatgg agattttaag
    17941 agtagtaatg taactgagtt ttttagagct tcttcagaat ctctaatttt aaatattaag
    18001 tataagtgtg gtaatcagat tttggaaaaa aacgtatgcc ctataggtat atatttaaat
    18061 aagggagcat ggttttcacc agcatatgat tgtaaaagtg ataaaataat attagttcat
    18121 atagataata taattgatat aaaaagaaca ggagaaagta aaaaaataca tataaacttg
    18181 gaagaatggt tacatgaagt atataacaat ttatatggaa atagttcaac tactaaaaaa
    18241 tttggtgaaa ttatttatct aaatgtatta ttaactaaag aaggtgtttc taaaataaag
    18301 gacagttctt ggaatttaga tatggagcta aatgaagatg gtagtggaat aattagtaca
    18361 ttcataaagg ttgaagatat agattgttat gcttcgattt tattttttat tggaaataat
    18421 gctaaggtta tagagccaaa atttttgaat aagctacttt atgataaggc caatgagtta
    18481 agatattttt atgaagaaaa tatggtataa tatttagaaa tgttgaaatt aaataattat
    18541 taaggagata tacgttataa tggaaaattt taataagaat gctaaaataa ttggtattgg
    18601 agctgaaggt attaatataa taaatgaagt agaagaaaaa ataaaagcaa atatggatat
    18661 agaaaaaata aacataaatc aagagattga aaaagaatat gttagaagtc ttttagatgg
    18721 tgtagatata ttatttttga tatattcatc tgaagataaa catattagag atattataaa
    18781 agctgtttca tatatgtcaa acgaaagaag agttctatct ataggaatgg attgttcaga
    18841 gaaagaaaat aaagaagatt tagagcttgg gagagagttt aaaatcaata atgatagtat
    18901 atttaaattt gttgatttaa tgaatatcat ggtagagtct atttctgatt cttgcatgat
    18961 aaatatagat attactgatt taaaagaagc tattgtagga gataaaggaa ttaagtattc
    19021 ttttgaagag tttgaagata caaagtcata tagcgagata gctgacattt tatttgacag
    19081 gatggaatat attggtgagg aatttataag taagaaagga attgtatttg ttgaaggaag
    19141 tccagaattt tctataatgg aattgaatga tttaataagt aatattcaga gcaaggtaga
    19201 agaaagctat gaagtaatat tttctttata tataaaggaa aatttaaatg gaaatattag
    19261 agtaggctta ttgtataatt agtatatttt agatattaag gagagtgtat atgtctgaaa
    19321 atataagagg tacagagtat tggttaatgg aagaattttt ttatcaggaa acagacacac
    19381 cacagatttt tagagaagaa agcataattg cacttggatt tgacataaaa ataaatttaa
    19441 aaaaagtatt cagtatgggg aaaaaatcag aaaagtatat taatgagaat tgtaatgata
    19501 aagattttat aaaaaagttt atgtcgatta aaaaaggaga ttatttttta tataaagcat
    19561 atcaaaatat agaagaaaaa gttaaatgta atgtgtctat tggcatagtt gaagaagatt
    19621 ttgaaaatgg atatgaatta agtcatattt tagggcatac actaccagta aaatggatgc
    19681 ataattttga tacagtttca aattgtaaag tatatacaag tgaattttat aaattaaata
    19741 ataatgatat tagctcatat ttagaaatta ttaagtcaca agataagaaa ttattgagaa
    19801 aaagaataga agaaagtgaa aataataaat ggtatttaaa aaactattat tataaaaatg
    19861 tagataagtg tgtagatgat gaagaattat ttattcagcc cattaatcta gaacaattag
    19921 aaagcctaaa aaatataaag acagatgatt atattttatt gtataatagc gagaaagtaa
    19981 tttgtgaaaa tgaccataca atgaagagtc tatttggcgt atgtcaagtt atggaagggt
    20041 tcgataaaga gaaagtaagt gctttggaag gtggatattt ttttcctgtt agatggatag
    20101 agtatgaaaa ggagaatata gacggaaatg catgggttat atttgaaaat aatagtatag
    20161 ttgaaaagta tgtaaaaaga tttactaata aaatgccatt aaatactata ctttatggac
    20221 caccaggaac tggtaaaaca tacaatacaa aacagatggt ttacaatcta gtaagtgaaa
    20281 ccagtctaag aaacaaatac tatacaggtt acttagatta taaaaacaat gtagaatttt
    20341 gtacatttca tcaatcatat ggatatgagg aatttataga gggtcttaag tctgatggtg
    20401 aaggcaattt taagcaagaa gatgggatat taaaggatat atctatagaa gcatgttata
    20461 atgcattgaa atttgaaaaa aagatgagct ttgagttgga aaaagatagc ttgactttag
    20521 aaactataaa agacaggaaa aagaaattag tattagaaaa tatagaatat agtgagagtt
    20581 ttaattttga agaatcagaa aactatgttt taataataga tgaaattaat agagggaatg
    20641 tatctaaaat atttggtgag ctaataacat tattggaaga tgataaaaga cttactaaat
    20701 caaatcaaac tattgtcaaa ctaccatatt caaaggaaaa attttctcta cctccaaatt
    20761 tgtatatagt tggaactatg aacacatcag ataaatcaat agcactttta gatatagctt
    20821 taagaagaag gtttaatttt gaagaaatta tgccagatta tgatttattg tctattgtag
    20881 atggtataga tattagaaaa atgctatata caatcaataa aaggatagag tttttatatg
    20941 atagagatca tgttattgga caagcttatc tacttgctgt agaaaatatt aatgatatag
    21001 tagtagtatt tagaagtaaa ataataccac ttcttcaaga gtatttttat tatgattcag
    21061 aaaaagtagg tttgatttta ggaggaattg gaagaaatga aaaagataaa tacattgttt
    21121 ataaagaaaa acttatagct tctgagttat ttaaaaataa taatgcctca ataatggaga
    21181 gtaaagttaa ctattgtata aaacaaaaca tatctagtga ggagctaaaa aatatatatg
    21241 agtaaaaata tat
//
LOCUS       DACULQ010000053        21010 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.053, whole genome shotgun sequence.
ACCESSION   DACULQ010000053 DACULQ010000000
VERSION     DACULQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21010
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.053"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(441..2828)
                     /locus_tag="JA154_08040"
     CDS             complement(441..2828)
                     /locus_tag="JA154_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAT7995816.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTDPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="JA154_08045"
     CDS             complement(2851..3216)
                     /locus_tag="JA154_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7995817.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="JA154_08050"
     CDS             3415..4263
                     /locus_tag="JA154_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995818.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="JA154_08055"
     CDS             complement(4700..5788)
                     /locus_tag="JA154_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995819.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="JA154_08060"
     CDS             5913..6641
                     /locus_tag="JA154_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HAT7995820.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6657..6839)
                     /locus_tag="JA154_08065"
                     /pseudo
     CDS             complement(6657..6839)
                     /locus_tag="JA154_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899621.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6843..7343)
                     /locus_tag="JA154_08070"
     CDS             complement(6843..7343)
                     /locus_tag="JA154_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995821.1"
                     /translation="MINMSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVS
                     IVIMEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIE
                     YFMKLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLR
                     KMLFKK"
     gene            complement(7345..7788)
                     /locus_tag="JA154_08075"
     CDS             complement(7345..7788)
                     /locus_tag="JA154_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995822.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7778..8617)
                     /locus_tag="JA154_08080"
     CDS             complement(7778..8617)
                     /locus_tag="JA154_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995823.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8836..10221)
                     /locus_tag="JA154_08085"
     CDS             complement(8836..10221)
                     /locus_tag="JA154_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7995824.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10394..11137)
                     /locus_tag="JA154_08090"
     CDS             complement(10394..11137)
                     /locus_tag="JA154_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAT7995825.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11157..12419)
                     /locus_tag="JA154_08095"
     CDS             complement(11157..12419)
                     /locus_tag="JA154_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAT7995826.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12603..13601)
                     /locus_tag="JA154_08100"
     CDS             complement(12603..13601)
                     /locus_tag="JA154_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAT7995827.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13585..15096)
                     /locus_tag="JA154_08105"
     CDS             complement(13585..15096)
                     /locus_tag="JA154_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086770.1"
                     /note="with RbsBCD acts to import ribose into the cell;
                     RbsA contains 2 ATP-binding domain; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAT7995828.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15150..16112)
                     /locus_tag="JA154_08110"
     CDS             complement(15150..16112)
                     /locus_tag="JA154_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAT7995829.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16160..17050)
                     /locus_tag="JA154_08115"
     CDS             complement(16160..17050)
                     /locus_tag="JA154_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAT7995830.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17092..18105)
                     /locus_tag="JA154_08120"
     CDS             complement(17092..18105)
                     /locus_tag="JA154_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAT7995831.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18333..19310)
                     /locus_tag="JA154_08125"
     CDS             complement(18333..19310)
                     /locus_tag="JA154_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAT7995832.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19516..19779)
                     /locus_tag="JA154_08130"
     CDS             complement(19516..19779)
                     /locus_tag="JA154_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995833.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19849..20922)
                     /locus_tag="JA154_08135"
     CDS             complement(19849..20922)
                     /locus_tag="JA154_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HAT7995834.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
BASE COUNT         6737 a         3858 c         2134 g         8281 t
ORIGIN      
        1 cttaaaaata attcgtcaat actttttaaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaacgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatacttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaaaaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctagaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgttaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggatctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcatgcc taacaatgtg cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggca tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctgtac aaggtatacc acaagctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtgttgg tgattcagaa
     4501 tagtatggta ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gcaaaaaaat
     6301 ataatgtaac cgattttcta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gaatatatta aatttagctt tatgctttaa tgtatattca
     6781 tatatatcat ttacacttat ctaaactaca atttcattaa aattaaatat aaaaatcaac
     6841 tattattttt taaatagcat ctttcttaat ctattattgt aataacctat aaaagaactg
     6901 taaacatagt catatactat aaaggctatc tcaaatgaac ctataataaa tatgtttatt
     6961 ggaatataaa ctattgttct taataaaaaa tacaatataa gtatcattat atttgcaaat
     7021 actaatttca taaaatactc aatatatata gacctatctt tttctataag atattttaca
     7081 ataccatata ttccaaaggt aagtgaatat acaatccatt gagacttact tgccatcact
     7141 ataaatccta aaactataga acctatataa aaagcaattc cactcttaaa gccccattcc
     7201 attattacaa ttgatactaa aagtgatgct aaccccataa taaacaatgt atttacagga
     7261 ataatgtttg taagagtcaa caatatcaca tttaatgcta ataaaactcc tccataagca
     7321 acttttatac tcatatttat catcctaaca acaagttatt aaatctccac caaaacattc
     7381 acaacatgaa tctaaacacc aaagtttagc acaattttca caacaatcgt cacctagaca
     7441 acaacagcaa tcatcacagc aacagcaaca acatccatct aaatcattac gtcttcttct
     7501 attataattg tcagccctat tgctataatc atttctatat tgtgtataac ttctaagtgc
     7561 atcactatat tcactatttt caggctccat aaattttgct ctttttaaga agtcttcacc
     7621 ttcttcataa tatcctatgt tcattgcaga aaaaccagtt aaataatacc actctgcaca
     7681 tttgtcagtc aaagttttta aaaaatcata tgctttttta aattcactat tttctatgaa
     7741 tgatcttgct ctcataaaat tttcattatt ataagtatta ctgtacattc tttccacctc
     7801 tactttctaa tatattaata tatcttaaat aaacaccaga ataaattata ttttctatta
     7861 ttccaacatt tttatttaga tttagattat caattcccct agctaaaaat cctaatgatg
     7921 ttactattat tttttttact ttttctttta actcatcatt tttatcaata tactctataa
     7981 atggattata tcttcctttc ttaaaatctt catctaaatc ttcataggca tctagtagat
     8041 atatatactt acctatatta aaacctatca ttcttaagtt tctctcattt tcatcttttt
     8101 tatagacaaa tacttcaccc attatatttc caaatatact agatacttca tctatattta
     8161 tatttttatc tttttctaac ttatttaatt catttagttg ttgttttata tactctgctt
     8221 tttcaggata cttttcatat gctaatttta atttgttttt atatatataa tatgccaatt
     8281 tatcttttat gcgtttatca tccattaaat tatcttccaa tttataatac gttaaaagaa
     8341 tattcatact tgctgcatat tctgtaatat catttgtaat tacctttttc tttttaaatg
     8401 gatttactat acacacttcc tctgtactta tagactctgg gttatatact gctgttaata
     8461 aaacaattaa aaatgttata tcatagttca aagaaaacct agagatttct ccatgatttc
     8521 tttttaagta tttacatagt ccacaatagt aagctttgta ataatcatat tctctaaatg
     8581 ttaaatccat tttgtttatt ttaacatatc caaacataaa tactcctttt tgtactattt
     8641 tttatttaga ttatatatag tataacactt tttatcaaga ttatataatt tacagtattc
     8701 taagaaactt aaaaaattcc ttctaatcat tgtatcttaa atttaaaata attagaaggt
     8761 tgggttaaaa ataatattaa attatatact tatattaact catacaagtt agttatgctt
     8821 atatcaattt aagttttaaa taattggtat atatttaaaa ctacctacag tatccttcaa
     8881 ttcttttttt gctttgttta taaccaattc atcaagcatt acatcattta ttgaacatga
     8941 tatagattta gatgccaata acattgcctt aaatcccata tcagaacctg cactggcaca
     9001 agattgccat gtatgggcag aaataccaat aggccaaact gaacaagaac actgtgctgt
     9061 aggaattatg tagctaacat ctccaatatc taatgataat gatatacagt tattatgagt
     9121 aaatgtatca tccgtaactt catcatgcag attcatctta gtaatatcct tatttccact
     9181 taatacacta gaaacactac ttcttttatc tatagacact gtctcagcta atttatctgc
     9241 aaaatagtag tcatcttcat tatagttttg cacacctact aatttcatat tttgtgatag
     9301 aatatctgtt aatattgtat ttggaatata gtcatatact ccacttataa tgtcatattc
     9361 cataatagta ccagtcatta atgctgctcc ttttgctata tcaacaatcc taccaaatac
     9421 atctcttaca tgctccattt taactcctct tatgtaatac cacacttctg caaaatcagg
     9481 aactatattt ggcatttttc caccattagt agttacatag tgtactctac aattatcaaa
     9541 tatatgttct ctcaaataat tacaacctat attcataagt tctactgcat caagtgcact
     9601 tcttccgtta taaggagctt gtgctgcatg ggcactaatc ccattatatc taaatttcac
     9661 agagtaatta gcaagtgttg gtattcttat tggagtattt atatcaaatg gatgccaact
     9721 aaaagcacaa tcaattccat caaaaaaacc tttttttatc atcaatactt ttccagataa
     9781 atcttcttct gctggacatc catagtattt tatagtccct ttcagctttt ttagtttgat
     9841 taattccttt actgcaatta cagctcctat tgaacctgta ccaagtaagt tatgtccaca
     9901 tccatgacct gcaccaccat ctacaatagg ttctttagta gtggatactt tttgagacaa
     9961 accatctaaa gcatcatatt ctcccaaaac tgctataact ggtctacctt ctccataact
    10021 agccacaaat gctgtatcca taccttctaa tccctctata ctaaaaccat gtttttttaa
    10081 acattctttt tgtaaatttg aagcatagta ttctttatac tgaagttctg gatttttcca
    10141 tatcttatct gatatatctc ttaattctcc ctgtatctca tctatatatt ccatcaaaaa
    10201 ctttttattt aaattatcca tattaattat ataaacctcc ctgtttctaa taacttatcg
    10261 attatcagcc aaaatgacta attttaactg atttaaaaat attttctacc atacaaccat
    10321 tttatacaat aaaacctctt gacttgtaag tttcatttta tcaagtaaga ggttttcaag
    10381 ttttataaaa tttttagcat gttaaccaat caagcatatt aacccataat ttattatagt
    10441 atttccaatc agtaaattct tttggacccc agtgaggtgc acagtcagaa ctaaagatag
    10501 cagattttcc ttttccaaac tcgccaacag ctacaaatgg gtctcctcca atagttgcta
    10561 aaacaggaca attatctcta gctactgttt tgttatatcc taaaaacttt ggccattctg
    10621 ttggtatacc ttttaatact ggatgttcag agtcaataac ttctggaatt attcctgctg
    10681 gtttttcaac tctatcatct ttatctatca ttgttattgg taatacatct ttgatagcag
    10741 tttctccaaa tctagctttt gcatctattc ctgtaaatga catatatcca cctaccatga
    10801 ttaacgcccc accattattt acatactctt tgattagttc aagtctatct ggattactat
    10861 tgcaatctat aaaagtacta ttagataata agaacgtatt agaacctata tcagataata
    10921 ttatacagtc atactcattt aactcttcta atttatacgg aaaactatct gctgctacat
    10981 gtgcaggcat atgaactgtc tcatatcctc cagattctat tgcttctttc aaccacttta
    11041 cagcttcttc ataatgagtt gtaacaaacg tatcaaaccc ttttatatga gttatatttt
    11101 ttacccaaga ttcccctact attaatactt ttttcattta tatattcctc ctacttttat
    11161 aagtttaata ttagacttgc atacataagt atactatcta aatactcctt aatttcaaca
    11221 tattcatcaa ttgaatgaca ttgttctatt gaacctggcc ctaatataag tgttggtatt
    11281 tcagctaaat ttttcattac tctcgcatca ttgccagctg ttgaaccttt tatctctaat
    11341 ttttttccta gagtttcttc tagtatttta tgtgcattca ctacaaaatc agaatccttg
    11401 tccatctcaa aacctaaccc tgattgatat atgtttatat ttggtttatt atctttaagc
    11461 cataaatctc cattagccct tgtcattatg acatttgtta catcatttac aacttgttca
    11521 taactcatta tatttggcaa gaagtgtaca cataaattaa atacacactt atctggcaca
    11581 gtagaacctg ctgttccacc atttattaca cctaaattta ttgtaggtga aggtaatagt
    11641 ggatgtttat atatcataag ccagttatgt tccaactctt ttatatcttg aagcaacaac
    11701 atagccttct caattgcatt tacaccctcc cacttactac cacagtgaag agacacacct
    11761 ttaacctcaa cttcaaagaa tacaaatccc atatgtgcaa ctattaagtt ttgttctgat
    11821 ggttcacaaa ttatgcaaca atctccatca ataccattca taactgcatt tatggtacca
    11881 tttccgccac cttcttcatc tactactgac attattttta cattaccagg tacatttaga
    11941 ccactatctt ttataagctt tacagctaat attgatgcta taagtccaga tttcatatca
    12001 gcagttccaa gaccataaag ttttccatta tcttctgtag cacggtatgg attatatttc
    12061 cacttactta tatcacccgg tggcatagtg tctacatgac cattaaatac aattgtctta
    12121 ccaggtaaat catcactata cttacaaatt aaattatatc tatcttgata attgtgacct
    12181 agatttcctt ctttatatat attttttgct tttttaatta attcttcgct catttcttgt
    12241 ctatccacac taaaacctat gtaatttgca agtttttcaa tatattcctg tccttctttt
    12301 tcaaatccac ctagtattcc atgaccaaca tcttcagtct ttatacttac aagttctttt
    12361 aaataatcta tgtattcttg ttttttacta tttaatgtat ctaagagaat ttttttcata
    12421 tttcctccta agatttattt ttacatttaa aatttcaagt ctaatctcat ctatttaagc
    12481 catagtaaac tttttcactt agcatattct cattcaatgt ataaagatgt atgcaaatta
    12541 aagtataaaa atatatacaa aaacttaata atttttatag tttataagct aaaatttaat
    12601 tactatttag atttagctga tttttttaat tttatattat ttatccatat agcaaataac
    12661 attaccaaac cttttataat catttgatta tatgaagata cacttaataa gtttaatcca
    12721 tttcctaata ttcctattat tccgacacct atacatgtac ctactataga accagaacca
    12781 cccatcatat tagtaccacc aagcacaacg gctgctatag catccaattc tgctccatct
    12841 ccaacagttg gtgaacctga tgccaaacgt gatgatagta ttatcccagc taatgctgtt
    12901 agtagtccag ctagtgcgta tacttttact ataactttat ttacttttac tccacataat
    12961 ctagcggcct cttcatttcc acctactgca tgtacatgac gtccaaaatt agttcttttt
    13021 aatagaaaat gtcctgctat tacaactata atcataatta taactggtat tggtattggt
    13081 ccaatatatc ctctaccgag catcttaaat gcatctgtat ttactgatat aggtcttcct
    13141 cctgtaaata caaagcttgc cccacttatt atcgttaaca tggctaaagt tacaatgaat
    13201 ggaggtagtt tttgtgttga aataattgca ccttttatga ctcctaatat tgtccctatt
    13261 gctaatgcaa gtatcatacc tacccatata ggtgcattat aattcactac tgttgtagcc
    13321 caaaatgtac ctactaaagc aattactgct cctacactta aatctattcc tcctgtaatt
    13381 attacaaatg tcatacctac tgcaagtatt gcatttattg atatttgtct tccaacatta
    13441 attaaattat ctaaagttaa aaagttagat gaagctacac tcattattat aaaaattatt
    13501 acaagtccta cagctgcact cccttggtct ctaaatacac ttgttaattt tgtcaataat
    13561 ggagaagatt tttcttcttt atgcttagtt attgaactca ttaattttac ctccgaaagc
    13621 caattttaat acattatttt caaccatttc ttcaccttct aaaaatccac ttatatgacc
    13681 ttctctcata accattattc tgtcactcat tgatattaat tctggtattt cagaagatat
    13741 cattattata cttacaccac tctccactaa atctcccatt agattgtata tctctgcctt
    13801 agcatttaca tctatacctc tggttggttc atccataatc aatatatctg gttgtaataa
    13861 aagccatctt gctattatag ctttttgttg gtttccacca cttaaacttt gtataacttg
    13921 attcatatgt ggtgttttta tctttaaagc ttctatgtat ttttctaaaa tctctttttc
    13981 cttttttcta tctataattg atttaaactt agatatcttt tttaacccag ccatagttgt
    14041 attttctttg acactcattt gtaaaaacaa tccagtacct tttctatctt ctgttattaa
    14101 tcctagtcca ttttctattg catctgatgg agttttaaac tttacttctt cattgtttaa
    14161 atatattttt ccagaatcat atgttctaag tccaaatata gcttctacta tttcacttct
    14221 cttacttcca actagtccac ctattcccaa tatttcacct tttcttatat taaagctaat
    14281 atcattaaaa acattgttct tagttagatt ttccactcgc ataatttctt cttttatttc
    14341 tacttttctt tttgggaaaa attggtctag ctttctatct atcattaaat taactaattc
    14401 ttcctcagac gatacatctt tggtatttaa agtcgaaatc gttttcccat ctcgcattat
    14461 agttattctg tcacaaatct caaagagttc ttccatccta tgggatatat atattatact
    14521 aactccactc tcttttaaat catttataag tctaaaaagt atttcagtct cttttttact
    14581 tattgaagaa gtaggttcat ccattattaa tactttagtt tctgatgata aaactcttgc
    14641 tatttctacc atttgttttt gagctatact aagctctttg actaaactat ttggatttat
    14701 atctattccc atattatcga aaatcttttt agcctcttca ttcatttttt tgtcattggt
    14761 taagactttg ccatttattt cttttcccat aaaaatattt tgtgctactg ttaattcgtc
    14821 aaaaactgtt agttcttgat atatggcatt tatacctatc tttttagaga tatctgggga
    14881 tatattctct atttctttgc cttcaaaaat aaattttcca gaagttttcg aatgtgcacc
    14941 agttaaaatc tttatcattg ttgatttacc agcaccattt tcacccagta gtgcatgaac
    15001 ttcgccatga aataactcaa aattaacatt atctaatgct ctgacaccag gaaattcttt
    15061 agcaatatta cttaacttca gaattatatt actcataatg acactccttt ttaaatagga
    15121 tttctttcaa ataatctcat tttattttac taattcttaa agtcttttat attttcctta
    15181 tcataaagta aagctggtac tggtatattc ttttctacct tttccccttt tatagctttt
    15241 actactgctt ctacagctaa acgtcccatc tctttaggtt gttgttgaat catagcaaga
    15301 atagttccat ctgttattcc acttgatgct tcttcagtta agtcaaatcc tacaatctta
    15361 acatccttat tttttgcaga ttcaagtgca gctttagctc ctagtacact attttcagct
    15421 gttgcatata ttatatctac atctggatta gattgaatta agttttctac tgtattaaga
    15481 gattcttctc tgtcatatcc tggttgtgaa tttaatatct taacatttgc tgaacctttt
    15541 aatgcatctt taaatccatc tattctctgc atttgtattt gagattttaa atctgtcact
    15601 attgctattt ctgactttcc acctaacttt tcatctatgt attttttagt gtattcccct
    15661 aattttttac cagcatcata actaactgtt cctacataac ttgtaatatt ttcagtattt
    15721 aatacagcat ctactgtaac tactggcatt ttttcaaatg ctccttctac tgctggtact
    15781 atagccgaat tatcagttcc aagtactatc attccatcta cattttgttg tataaaatct
    15841 tctattgctg atgtttgctt agctacatct tggtctgggt catttactat aactttcaca
    15901 cctaattctt tagctttttc ttttattccc tcatcaatat ctataaaaaa ttgatgttga
    15961 agagttattg tagatactcc aatagttaaa tctttcttgt ctgaaccacc tttattatct
    16021 gatttacttg gtccttccat tgaacaccct gttaacattc ctattgctag tattccagca
    16081 attcctaaag ataaaatttt tctcactctc ataattaacc tccattttta atagttttaa
    16141 atttgaacaa attagatttt tatttattat ttaaatattt ttcaacatct tctttataag
    16201 gcataccaga ttgtgcacct agtttagtta cagacagacc tgatactact actgcatatt
    16261 ctactgctgt atataaattt ttcccttctg ttatagccac tgccaatgct ccattaaatg
    16321 tatctcctgc tcctgtggta tcttgaacat caacacttat acaagataca tgcttgattt
    16381 cctcaccatc ataaaatcta gctccatttt ttccttctgt tattacaagt ttatttggat
    16441 atttttttaa tacttcttct atcccttcat ttgtgtcaaa aactatctta tattcatgtt
    16501 catttggtgt taagtaagtt actttttcta ttatatcttc atttagtttt actgctggtg
    16561 ctggatttaa taaaacccta atcctatttt caaaacagaa attcactaca taatttattg
    16621 tttttaatgg aatctctaat tgtagcaata ctatatctgc attctttatc tcttcttcat
    16681 tctttttaat taattctata tctactagct cattagtccc tggtacaaca acaatactat
    16741 tatcattttc acataatgtt ataacagcaa caccagaact tttgtgatta tttctggata
    16801 ctaagctata atcaacttta ttcgttctaa aatttcttat taactcctct cctaagccat
    16861 cttcccctat gcatgatatc atttttacat ttgctcctag tttagaagct gagatagctt
    16921 gattagcccc ttttcctcca ggtgatgtaa aaaaactatt acctaatact gtttctcctt
    16981 tttctggtct tttatctaca gaacatacca tgtccatgtt tacacttcct atgacaacta
    17041 tgtttcccat tacattactc ctttacatat ctatttatgt ctctcgtgct gttattcact
    17101 ttcttttgtt gaatctctta tgaccaattc tggtttgaaa atcaattcat caaacgattc
    17161 ttttttatct cctcttaaac aatttatcaa tatttcactg gctttatatc ctatatcata
    17221 agacggctgt tttactgtgg taagagaagg gcttattaga cttgatgttt gaatatcatc
    17281 aaatcccacg acacttatat catttggtat acttaatcct ctttctttaa gacctcttat
    17341 gacacctatt gcaatcaaat catttgcaca aaatattgca tcaaaatcta tcttttctaa
    17401 attttttata aattcttgtc cccattcaat cttatatttt ccttcaaata cataagcagg
    17461 attgtagctt aaattgcact cgattagagc ctttttatat ccttctaatc tatctactgc
    17521 tatttcattt tgaagtggac cacttaggta tattattttc ttatttccaa tatctaataa
    17581 atgttttgta gctatatagg ccccttcaaa gttatttatt ttaacaattc cttttaaggt
    17641 ttctttgttc atacttatct tcttatcaat caagacagtt ggtattttta tatctttata
    17701 atttgcttct ttccagtttg tatttgaaga tgctgcaaat ataattccat ctatcatcct
    17761 ctctgatagt gttcttattc cttcatattc ttttttagca tcttcatctg tattacataa
    17821 aattatattg tagccttctt cattagcttt gtcttctaca cctcttgata catctgtaaa
    17881 gaatggattt cgtatatctg gtataagaag ccctatagta ttagtttttt tagttactaa
    17941 actttgtgct aatttatttg gtacataatt tttttccttc attattttca aaattttttc
    18001 tcttgttgcc tgacttatat tttcatcttt attattaatg acttttgaca cagttgtttt
    18061 agaaacacca gccaattcgc caatttcttt tatagttata ctcaagaagt tcacccccaa
    18121 aatttaggaa accgttttac ttaaccggtt acctagatac tatcacatct tttttccatt
    18181 tgcaaccatt ttatataaat tttgatattt ttcccttttt tcaaaatatt gcaacttata
    18241 attcaatttt aatatttaaa ttaatctgaa aaaaaaatta atatactttt ataattatct
    18301 aaaagtatat taattaattt aagcagtatt tattaaagtt caaatccttg ctctataatc
    18361 atttttttat cattttcaat cccacttcca acagttgtca aataatcacc aacaattgca
    18421 gaatttactc ccccattaaa tccaactttg tcatatccct ttaatgatag ccttccacca
    18481 gcatacctta tgtacacatt aggaattata aacctaaata ctgctgttgt ttttagtatt
    18541 tccattggtt ctaatacatc ataattttcc attggtgtat tcttaatagg atttaaaatg
    18601 tttattggaa atgattttac atttaattct cgtatttcaa aagccatttt tagtctctgt
    18661 tcttcactct cattcattcc aattataccg cctacacata catctagccc agctttttta
    18721 gcatctttta tagtttttat cctatcttta tatgtgtgtg ttgtacatat attatgaaaa
    18781 ttatctctac acgtctcaac attgtggtga tatgtagtca cacctgctga cttcaacatt
    18841 ttagcttgct cataatctat aatacctaaa gatgcacata attttaaatt agtattttcc
    18901 ctcaatcctt catagatatt taaaatatta ttaaactcct ttcctgacat acctttacca
    18961 cttgtaacca atgagaatct atgcactcct ttactctcca tctctttagc tctatttaag
    19021 atttcattgt aatctaataa tgaatattct tctacaccag ttttataata agaagactga
    19081 gcacaaaatt tacagtcttc actacatttg ccagatttag cattcattat cgtacataaa
    19141 tctgcttttc ttcccataaa atattctctt atttcatttg cagattttaa tagaatatca
    19201 aaatcgtttt tattatttgt atcaaggctt attaaattaa gagcttcttc ataacttatt
    19261 tctttttctc tcaatacttt attttttaat tttattatgt atttttccat atatcctcct
    19321 atttgtcaac ttaatatttt tatgtagttt acttattata tatagtattg catatataac
    19381 atttattggt caactttatt ttagacacaa gtttactata cacaaaaaaa tactgcttca
    19441 aaacaatatt agtatataga ttatacatat tattattaaa gcagtatttt ttattagaat
    19501 ataattctta ctggtttata aaactatcct tgtcttaact ccatccaagc tttccattgt
    19561 aataacttct aagccttcat attctctaaa tagccatttt aaatccttca tggcttgatt
    19621 aacctgactt tttgttagat tatttataaa atattctttt ctagtttcct catactcttt
    19681 gctagtcaaa aagtttctta gaaaattaaa cgcaaataca cttggaatat tgtttacatt
    19741 agccttcttg ctgttttcct tctcatagta aattttcata aatgcatact cctttgatac
    19801 ttttagattt ctcttacaat aattatatgc atttaagtgc tactattctt ataattttat
    19861 taattcatat tctttacttc ccatacccat ttcacatgca tagtctatta ttctagttcc
    19921 atttacatga ggatgttcat gtttgaaaac atcatgaccc atttccttac aaatcaaatc
    19981 atagcatgct tgttcaatag ctacagggtc tgttgatgct aatattccta tatcatgggc
    20041 aattggtcta cctgaccaag gtacacaatc acaaagtgga gttacattca taacaaaagt
    20101 tatataccct accttgtctt ttttatttga tactgctcca taagcgtatt ctgccatctt
    20161 ttcgacaaac acatctgagt cactttccca ttgtatagtt actgctctag ttggacagac
    20221 agttggacat tctccacatc cataacatac atcagaatct ataactgctt ttttatccac
    20281 tatacttata gcttttgtag gacaagaatt aacacacttt ccacaaccta cacacttctt
    20341 ttcctttact actggagtaa catcagaatg ttgcatttgt tttcctgctg cacttgcaca
    20401 tcccatagct aagtttttta atgctcctcc aaaacctgct gcttcatgac ctttaaaatg
    20461 actcattact atcattgcag aagaattata aatttctcca cctattttta cactttcaaa
    20521 atgttttttg tctattttta cattctcata atttctacta tataatccat ctgcaattat
    20581 tactggtgca tttacaactg cataagcaaa tccattttct atagcagttg taagatggtc
    20641 tacagaattt gttctactcc ctgtatacag tgtatttgta tctgttaaaa atggctttcc
    20701 ttcgcagtct ttaaccttat caaccacttg tctaactgcc acaggactca tatatgtagt
    20761 atttcccttt tctccaaagt gaagtttaat tgcgacttgg tcattttttt ctattaaatc
    20821 tttgaatcct gcttcatcaa ataatcttct tacattgttt ggtacgtttt tattttgcga
    20881 atttgagtat aaatcacaaa aatatacttt tgacttttcc atatctacac ccctttaaaa
    20941 tatttactta tactaatttt atcactttta aagtaatttt tagtatattt taacataatt
    21001 aaatattctt
//
LOCUS       DACULQ010000054        20953 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.054, whole genome shotgun sequence.
ACCESSION   DACULQ010000054 DACULQ010000000
VERSION     DACULQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.054"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            161..1171
                     /locus_tag="JA154_08140"
     CDS             161..1171
                     /locus_tag="JA154_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995835.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            1282..1770
                     /locus_tag="JA154_08145"
     CDS             1282..1770
                     /locus_tag="JA154_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAT7995836.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1946..3874
                     /locus_tag="JA154_08150"
     CDS             1946..3874
                     /locus_tag="JA154_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF-domain containing protein"
                     /protein_id="HAT7995837.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            4051..4461
                     /locus_tag="JA154_08155"
     CDS             4051..4461
                     /locus_tag="JA154_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAT7995838.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4483..4785
                     /locus_tag="JA154_08160"
     CDS             4483..4785
                     /locus_tag="JA154_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HAT7995839.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(5002..5694)
                     /locus_tag="JA154_08165"
     CDS             complement(5002..5694)
                     /locus_tag="JA154_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAT7995840.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5720..6907)
                     /locus_tag="JA154_08170"
     CDS             complement(5720..6907)
                     /locus_tag="JA154_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7995841.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            7243..9120
                     /locus_tag="JA154_08175"
     CDS             7243..9120
                     /locus_tag="JA154_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit EIIBCA"
                     /protein_id="HAT7995842.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKVGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            9430..10836
                     /locus_tag="JA154_08180"
     CDS             9430..10836
                     /locus_tag="JA154_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAT7995843.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDIYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            10864..11712
                     /locus_tag="JA154_08185"
     CDS             10864..11712
                     /locus_tag="JA154_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7995844.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNEKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            12214..12906
                     /locus_tag="JA154_08190"
     CDS             12214..12906
                     /locus_tag="JA154_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7995845.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            12903..13838
                     /locus_tag="JA154_08195"
     CDS             12903..13838
                     /locus_tag="JA154_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7995846.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            13986..14909
                     /locus_tag="JA154_08200"
     CDS             13986..14909
                     /locus_tag="JA154_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7995847.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            14902..15663
                     /locus_tag="JA154_08205"
     CDS             14902..15663
                     /locus_tag="JA154_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7995848.1"
                     /translation="MLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNLPFE
                     QLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLAVLL
                     FISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYFNRS
                     YIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEYTVL
                     RQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            15673..16455
                     /locus_tag="JA154_08210"
     CDS             15673..16455
                     /locus_tag="JA154_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7995849.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVILVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            16832..17368
                     /locus_tag="JA154_08215"
     CDS             16832..17368
                     /locus_tag="JA154_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HAT7995850.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            17400..17789
                     /locus_tag="JA154_08220"
     CDS             17400..17789
                     /locus_tag="JA154_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAT7995851.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            17826..18212
                     /locus_tag="JA154_08225"
     CDS             17826..18212
                     /locus_tag="JA154_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HAT7995852.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            18339..19781
                     /locus_tag="JA154_08230"
     CDS             18339..19781
                     /locus_tag="JA154_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HAT7995853.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            20078..20893
                     /locus_tag="JA154_08235"
     CDS             20078..20893
                     /locus_tag="JA154_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT7995854.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         7732 a         2455 c         3865 g         6901 t
ORIGIN      
        1 ggtattgtat actggaatta gagtaattaa tttatttttt aacatacaaa tgattttatg
       61 aaaaaaaaca agactcaaaa atttttatat tctttgatag aataaaattg agagtaaatt
      121 tattgaattt tataaaattt ataaggggtg agtctaagat atgcatgaag ataaaaatga
      181 tatatttgaa tcaatagata ctgaggattt tgataataaa acagaagaag aatgtttaga
      241 gctagtaaga aaaaatggat tgaatttaat atacataaaa aatcaaacag aagagatatg
      301 tctagaggct gttagacaaa attgtaatgc aataaaatgt gttcagaatc agacagaaaa
      361 aatatgtata gaagcagtta aacaagattg gagaatgtta gaatttgtag aagagccaac
      421 agaagaaatt tgcatgttgg ctattaacca agatggaaca gcattaaaat atatagaaaa
      481 tcagacagag gagttatgct taagagccgt tgaaaaaaat ggtgcagctt tggagtatgt
      541 aaaagaacaa acagaagaaa tatgtataga agcagttaga aacagcgaat ttggattggc
      601 aagagtgaaa aatcaaacag aaaaaatatg tatggaagca gttaagcatt gtagctataa
      661 tctaaaatgg gtagaaaatc aaacagaaga aatatgtatg gaagcagtga gacaaaatgg
      721 gcttgatttg aaatttgtaa aaaatcaaac agaaacaatc tgtttaagag cagtaagaca
      781 aaatggaatg gcattagaat ttgtaaagga acagactgtt ggtatatgct taaaggcagt
      841 aagacaatat ggaatggcat taaaatttgt aaaagaacaa acagaagaaa tttgtacaga
      901 agctataaaa caagataaaa gagcacttag ttttgtaaaa ggagataagg aaaaatacaa
      961 ggctctatat gataataatg aaccatttgc aaagagatat gttagaaatg taatagaaaa
     1021 agaaaacaga gcattgatta aaaaaggaga agaaaatgca aaaatcaaaa ttgcaggaaa
     1081 actttatgat agaggcatgt cttttgaaga tatatcagat atagttgaga ttgaaataag
     1141 caagttaaaa gtgagtttag gtgtttttta agggaaatag ttaataaaaa aataatcgac
     1201 tagctctttg taggacagca aaattagtga tataatttga tgtataataa attattttta
     1261 ttagataagg gggaattaaa gttgaagatt acagtattat accaaacaag aagtggaaac
     1321 acagaaaggg tcgctaagct gatagaagag ggcgctaaaa aggtggatgg agtagatgta
     1381 aagcttatga gacttgataa tattgattta gattatttaa atgaaagtaa agcagtaata
     1441 tttggaacac ctacttattt agcaaataca acttgggaag taaaaaaatg gtttgatgaa
     1501 gattcaaaaa aagttaattt agctggaaaa ttaggagctg tttttgctac atgtgattat
     1561 atatgtggag gtccagatgt tgcgatactt actatagtag gacatttgat ggttaaaggg
     1621 atgcttgtgt attctggagg tggttcttta ggaaagccat ttatacattt aggacatgta
     1681 cattcaattg aaggtccaga gttacaagat gagaaggcta taatatttgg tgaaagaatt
     1741 gctactaagg caaaagaact atttgcttaa tttctatatg cataataatg attagagtaa
     1801 atttagggtt ttaatggaga tttgaagtaa aagttataaa ataaaaaatt ttccataaaa
     1861 ttactcaaga tgaattataa tataattata aataaattat attattaaaa tatttaaaat
     1921 caaacgtcaa tagcgaggga agtttatggt atggcatata gaatatgaga ttttttctgc
     1981 aattatagtt atttttttaa tggtatactt ttttagaagt aaatttatac ctactttaca
     2041 aaacaaaata tattgtgcac ttttaatatt ctcattttta tttataattt caaatatttt
     2101 aggttcattt tgtctaaata atatagacaa aattccaatt tttattactt ttttattgaa
     2161 tcagatatac ttattgttat tgccaatacc agcagcactt gtatcatttt atgttatggc
     2221 tattatttat caagatataa ggtatatgaa aaaaagatta ttatttttat ctataccact
     2281 tataatttgt ataatcttat ctattacaaa tccttttacg catattctat ttggattgtc
     2341 aaaagaaaca ggttatatac gaggttgtgg atatgttgca acttttgcat cttttttctt
     2401 ttatgcaata tattcagcat ttttatcttt taaatataga aaagcaatgc atgaatcaaa
     2461 aatatgggct attagaggtt ttcttatagt ttcaattgtg gcaattatct tacaggcaat
     2521 ttttttacag tatcttctta caggaactgc atgtgcatgt agtttacttt tgacatatct
     2581 ttctatgcag aatcgaggtc ttattattga tgaccttact ggtgtactca atagacaatc
     2641 ttttattcaa gagctagatt tgaatatcaa tactgaacaa tcaggattta ttattacaat
     2701 tgcgcttgat gattttaagt ttatgaatga aacttttgga acaaaaaatg gagatattgc
     2761 actaaaagaa gttggaaaat atttgattca gatacatgat gtaaaccatg tatatagaat
     2821 agggggagat atattttcta ttgttgtcaa tagtcaatta ggtatagaac cagatgatat
     2881 tatcagaaag atagaagaaa gatttaaaaa gccttggcta gtagaagaga taagttttaa
     2941 tttagcaact agtattgctg ttgtttatta cccagaaaat gcagaaacga cagaagatgt
     3001 aattgttgct atagatttct ctatacatga agcaaaacgt tctgagtcaa ggcgagttgt
     3061 ttatgcagat gcgtctataa gtgaaaagat aaagaagaaa catgttatta aaaattgttt
     3121 aaaagaagca ttaaaaaatg atgggtttga agtgtactac caacctatat tttctaatag
     3181 caggggcagg tttacttcag cagaggcatt acttaggcta gaacacaaag agttaggttt
     3241 tatacctcct gatgaattta ttccaattgc agaacaaaca ggattgatta actcaattgg
     3301 attagtagtc tttgagaagg tatgtagatt tatagctagt gatgagtttg aagatttaaa
     3361 attggataat atagctgtga atctttctgt agtacaatgt atgcaaaaaa acttagctga
     3421 tgatttactt tatcttatga ataaatataa tgtatcacca actaaattta aacttgaaat
     3481 tactgagact gtggctatgg gctcttttaa tataattaaa gaaacaatgg aaagattaat
     3541 tgatttaggt gttaagtttg cacttgatga ttttggaatt gcttattctg gagttactaa
     3601 tatgctttct ttaccatttt cagtaattaa gttagataag agtttgattt ggtctatgaa
     3661 tgaagatagt aggcataaac ttacagttga gacgattata gctcttataa ataaattgaa
     3721 tatgaaggct gtagcagaag gtgttgaaac cattaagtgt gctgaagatt taatagctat
     3781 ggagtgtgaa tatttgcaag ggtattattt ttctaaacca gttccagaag atgtttttaa
     3841 aaatttgttg aaagaaaata cttttacaag ttaacaccct ttattttttt aattacaact
     3901 ttgttcattg acactgccag cgatatatta taaaattaac acaataataa atattaaatt
     3961 tattatacat attattaata ttaattaatg gagtaaagat ttattatata ttttatttaa
     4021 ttatcctaaa gtgagaaagg aagatgtgac atgaataaaa agaaaatatt agtgccaatt
     4081 gatgggacag aaagaagtat gcattctttg gaatttataa aaggaatatt taaaaaagat
     4141 gaagttgaag tagaaataat gaatgtaaaa gaactagtat ttattgatgg catatccctg
     4201 gctgaagaga taaaaaattc tgaaaattta ggaagaagaa tcttggataa agcagcagag
     4261 ataatgggag agtatgatgt taaagtacat tttacttttg gatatccagg agatgagata
     4321 attagaaaag caaaagaaga taatgtagat tttatagtta tgacaaaatc tactaaaaaa
     4381 ggtcttacaa gaatgatagg ttcagtaaca gcaagtgtag ttaaacaagc aaaatgtata
     4441 gtgatgattg taccggagta gaattagaaa ggtatttgaa atatgaataa aaagatagta
     4501 gcagtttgtg aaagtgaaag aagtttaaat tctcttaaga cagctgcaaa tgatttagga
     4561 tttgatttag tttatgaaat tcaagaaaat gataatataa aaaatgaact ttctatttat
     4621 gatatagaag atgcatccat agtgttattt gttacaagtg attctataga aagcatagag
     4681 aagattgata gatttataga tagagaattt tatgaagttg atccaaagta tataatagag
     4741 gatgcaaaat ctatagtaag tgaaatttta atagacttaa attaatcgtg ggtgtcgcac
     4801 taagaataga gcgacgcctt tttatatatt ttacacaaga aataaagagg ttgtatcaaa
     4861 atatagataa gagtcttttt cattgttgaa atgatgaaaa tatatttgat ttcatacaaa
     4921 aaagagtacc tcataactaa aaagttcact ttgagacact ccatttttat atgttagttg
     4981 catttaaggt aaaatagaag tttaatcatg ttgaaataac tgaattacat cttctaaatg
     5041 attgtacatt gatgtataac ctaagtaaga gtctttacta tcaaatgcaa tcattatctt
     5101 gttatggtgg ctaatcatat ccttgagaga atttctatat ttaaaaccaa agtattggtt
     5161 agaagaaact gttttaggta ttaactcata atattttata aaaattggat tatgagaagc
     5221 taaaattatc tctttgtgaa attgcaagtc agcatcataa cattcttcta tcaaaccgtg
     5281 agaaacctta tccaattctt ctattgactt taggtaagta tattttattt tttgaagttc
     5341 tttagatgta gcatttaaaa ttgctagttt agtagcttca ctttcgataa gttgtcttgc
     5401 tcttaaagat tctacataaa attcttgtat gttatgattt ttatagattt caccatcatc
     5461 aaataggtca acttttttaa atccttcaac tataggactt gaataaggca cagctctaag
     5521 tattccttcc ttacagagta tatttatggc atttcttaca ggtattctac ttatgttaaa
     5581 taggtcagca atctttctct cacttggtaa tttactatta attttaaaag ttccatttag
     5641 aatgtttaat ttaatataat caataatttt ttcagtggca tttaagcttt tcataaaaac
     5701 tcctaaacat ataatttttt tatgattgta ttatatcata gttaagagtt aaatctactt
     5761 ttttataata tgtataagac aggatagagg ccaaaatagc tataatagcc aagataaagt
     5821 atccatattg tattgaagta tattccgata ttttaccaac tatcattgga aatacagaca
     5881 ttcctatagc ataaattgct tgaaaaaaac ccattgcagt agatttctga tgttttggta
     5941 catttttcat agactcgcta gtaaggtaag aaaggagtat acctgtagat atacctggta
     6001 gtacttgtag tacaaatatt atattaatat tttttattgt tggaactaaa aagcaatagg
     6061 ctgaaactaa aataaaaact aaaggaatcc aaaattttgg cccatactta gtacaaaatt
     6121 tagtagaagc aaagccagaa gaagcaacag cacacaccat gtaaaatata gaagatagac
     6181 ctatgagggt agaactagaa cctaagtttt taagaatttg agtagtaaat gacattgttg
     6241 tagacatttg aataccctgt tgaattaatg ccataaagga aaaaaataca agtcttttat
     6301 ccttgcagat agataaatat tttttaattg atattttttc aggtattttt tttgaattat
     6361 gattttttat atttaaggct agaatcgttc caataactga agacaataca ctcatgatac
     6421 ataaggctgt cattcctaat ttttcataaa ataaagtact cataacaaat ccaatcaaca
     6481 ttcctagatt gtttactaat attattgtac ttgtagcttt ttgttgatgt tctgtagaaa
     6541 aaaagttggt atagtgaacc atgtaagaaa tccaagttga tgatgatatt ccagataaaa
     6601 tgttagctat aagaaaacca ttgcctccag gagaaaagac tcttataaga gatgctaaac
     6661 cagcaaagaa aattccagac agtataaata ttttatgttt atttatggaa tcagcaaata
     6721 ctccaacagg aagccttaat aaacattgtg agattccata tgcacctatt attataccaa
     6781 tcatatttgt agatacatta catgcactta aatatggatt ttggaaaggt atatatacat
     6841 attgtgaaaa ccagaaaaat gtaacaatag aaagtaaaag taatttttga ttagatattt
     6901 ttttcatatt atcctccgta tggtattttt ataatgcaaa taatatacca tttggatttt
     6961 ttaatcaagt actttctcta ttaaaaaaaa tttaaatata taattattat ttacaaaaaa
     7021 attatacttt atagttgaat taagaaaacg cttatgatac tataaagtta acaaatttga
     7081 atatataaca gtagacgtgt tactggtaat gcaggcaaga tcgaatcaat ttttatagat
     7141 tatctataga gtactatggg tataatctat ttatttggtt cgattttttt gtttaattgg
     7201 ccataaaaca aaaagcctat agttgaaaat ggaggatttt ttatggatta taaaaatata
     7261 gctcaagaaa ttctactaaa tgtaggagga aaagagaatg taaatgaagt gactcattgt
     7321 atgacaagac ttagatttaa agtcaaaagc gcttctaaag tgaataagga taaattatct
     7381 aaaactgaag gagtaataac tgttgttgag agtatgggtc aaatacaagt tgtaattgga
     7441 aataaagtaa aaaaagtata tgatgaggtt ataaaaattg tacctcaatc aaataacgta
     7501 ggtaaaaaga atgaaagtca agaaaagcaa ggtataatta attctatatt atcagcagta
     7561 gctggtatat ttacacctac aatacctgct atagcagggg ttggtatgat taaaggtatt
     7621 ttatcagttc ttgctatgta ttatatgaat aaaaatggag tagatataaa agaaacgcaa
     7681 agctatatta tcttaaatgc catggctgat tctatattct attttatgcc tattatacta
     7741 ggatatactg cggctaaagt atttaatgca aataaaataa tatctatggt attaggagct
     7801 acactttgtt atccaacttt tactgctttg atggcaggtg aagaatctgt tagattttta
     7861 ggattggcag taacaaaagc tacgtataca tcatctgtta ttccaataat tattgctata
     7921 tgggctcttt catacgttga aaaagtatta gaaaagtata tacctgaggt aattaaaata
     7981 ataatggttc caactttatc attggtagta atgttacctg caacattatt tttatttgga
     8041 ccaatcggaa tttatatagg aaatgtcata aacttttctt ataagtatat ttatgaatta
     8101 agcccagcat tgtgtggagc atttgtaggt gggttatggt gtgtgttggt tatatttggt
     8161 gctcatagag cattattacc aataggtata agtgatgttg ctcaaacagg aagacagaat
     8221 ttattggcat ttgcaggagc tgcaaacttt tcacaagcag gggctgctct aggagtattc
     8281 tttaaaacta aaaatcaagg actaaaaaca atttctatgt ctgcaactat aacagcttta
     8341 tttggtataa ctgaacctgc aatatacggt gccaatctta gattaaaaaa gcctatggta
     8401 tgtgccgtaa tatgtggtgc atttggaggt gcgataatgg gaatgggtgg agcttatggt
     8461 aatgcatttg caaaccaagg tgttttaact atacctgtat atgctgaagc aggtgcatta
     8521 ggtttcttaa gttatctagg tggttgtgct atagcatttt ttggttcagc tatttcaact
     8581 tatctagttg gatttgaaga tttggaagag tcttctaata aggaaaattc atctataaaa
     8641 gtagaaacga aggatggtgt tattgatata acttcacctg tagagggcga atgtatagaa
     8701 ttgtcagaag taaaggatga tgtatttgct tcaaaagcta tgggagaagg tatagctgtg
     8761 ttacctacaa aaggagtaat aacagcccct actgattgtg aagtagcttc tttatttcca
     8821 acattacatg caataggatt aaaattagat aatggagcag aaatgttaat acatgttgga
     8881 atcaatacag tagagttaaa tggtaaatac tttaccaaac atgttaatca aggagattta
     8941 gtgaaaaaag gtgataaact tatatcgttt gatatagata aaataaaaaa ggttggttat
     9001 gatgtaacta cgccagttat agtaaacaat acatttgatt ttgggcaagt agtttcttgt
     9061 aaatctagtt atgtttcaac caatgataat ataatttcat tagtattagc tggtaattaa
     9121 gttaatttga ctagagataa gtaaattgat ttaaaaggaa ataagtaaat caacaattaa
     9181 atacatctaa aagtaagttt attgcaatga attgttaaag aaaataatta atatggagta
     9241 aatctattgc aatgaagtaa caaagaaaat aattaatata gagtaagttt attgtaatga
     9301 attgttaaag aaaataatta atatagggta aatctatttc aatgaaataa caaagaaaag
     9361 agttaatata gagtgaactt attgcaatga atcaacaaag aaaataatta ataaaaagga
     9421 gaaatattta tgaatacagg atttccaaaa gactttttat ggggagcatc atcttctgca
     9481 tttcaagtgg aaggagcttg ggataaagac aataaaggaa aaaccgtagc cgactataat
     9541 tcttttaaaa agtctcattt acaggcagat actaaagtcg catcagactt ttaccataat
     9601 tatgaagaag atattgaatt aatgaaagaa ttagggatga aaacatatcg tttttcaatt
     9661 tcatgggcaa ggataatacc agatggtgaa ggagagataa accagaaagg tttagacttt
     9721 tataataaga taatagacaa actaatagag tgtgatattg agccattcgt gactttatac
     9781 cattttgacc ttccatttaa attagtagag aaatataatg gttgggaaag tagagaaact
     9841 gtctatgctt ttgaaagatt tgctaaaata tgctttaagc attttggaga tagggtaaag
     9901 tattggcaac ctcataatga acagaattta attgttagag tggaggagag aattaatata
     9961 tatgatgaaa cagattcatg gaagatagat aaaatgcgtg ctcagatgga ttataatctt
    10021 tgcttagcac atgcattagc agtcaatgca tgtcatgaga tgataaaaga aagtaaaatt
    10081 ggagcagcag tatcatcatc tgtaacatat cctcttactt ctaagccaga agatatatat
    10141 gcagctagaa tgaatgataa ttttaaagtg tactatatgc ttgatatgca tcattatgga
    10201 gaatatccag ggtattatat gaagtatctt gaaaaaagaa atatagttcc tcacatggaa
    10261 gatggggata aagagatact taaaaaagct aaaatggatt ttatagctgt aaattattat
    10321 aggacaaatt gtgcagaagc tttacctgaa gattcacaac atccatttgg attaagagaa
    10381 ggaactgttg attttagtat gtatggacta tttaaaatgt caatgaatcc taacttagag
    10441 gcttctgagt atggagcagc aatagaccca tcaggtctta gagttgcctt aaatgaatat
    10501 tggcaaagat atcacttacc tgttataata acagaaaatg gtctaggagc taaagatata
    10561 ttagaagatg gaaaaattca tgatgactat cgtatagact atttaagaag tcatataaat
    10621 gcatgtaaac tagctattga agatggtgta gagatgattg gatattgtcc atggtcattt
    10681 acagaccttt taagttcttc tcaaggtttt aataagcgtt atggacttgt ttatataaat
    10741 agaactgacc atgaaatttt agatttaaaa agaatcaaga aagatagttt ttactggtat
    10801 aaagaagtta tagagaacaa tggaatagta aaataaacat aaatatatat tgagggtttt
    10861 agtatgtaca ttacaaaggt attaaataat tcgttgctac ttgcaaagga tgataatgat
    10921 gaagaaataa tattgatggg gaaaggcatt ggccataatt ataaggccgg gtctgagtta
    10981 aatgaaaaag atatagaaaa gatttttgtt cttcatgatg aaaatataaa aaagagtatt
    11041 atacaattgg caacagaaat agatgcagaa tattttggta tagctcaaat gataattgca
    11101 tatggaatag aaaaatataa cttaaaactt atgaaccata tatatttggc cttaactgac
    11161 cacattgcct ttgcagtgag acgttttaaa gaaggtataa tgattgaaaa tcactatttg
    11221 ttcgaaatca aggaatttaa tccaaaagag tatgatatag gaaaatatgc tattactgtt
    11281 tttaaggagg ttcttggttt agacctacca gaagaagaga ttggtaatat agcaggccat
    11341 tttataaatg cacaacaaga taatccttat agtgatagaa ataagagaag tgctaaaatt
    11401 gtaaatgcta ttttgcaaat agttcattat cacttttcta ttgtatataa taaagagtca
    11461 ttttattata ggagatttgt aatgcatcta aaggcctttt cacaaagatt tctggctaat
    11521 gaaccatcaa gagaaaaaat agattttata tatgagcaag ttcaaaaaaa ttgtaaggaa
    11581 gagtatgaat gtgtgaaaaa ggtaggagct tatatgtata aggaatactc cagagaatta
    11641 ccaagacaag aggagttata tttgatgatt catattcata agattttagg agaattatgc
    11701 gaaaatagtt agaatatctt aatatgctag taatattata aaaaattaaa aattaactgc
    11761 actttaaata tgaataattg cattacttaa gggggttgtc ttaaaattga tttttttaat
    11821 taaatttgaa gctcaactcc ttttttaaac ctttatatca ttacaacttt aatgtttata
    11881 cgcctgtact tcagatgtat atctactttt tacatttctc aacatgcaaa tctaccattc
    11941 tgtatatagt aaatgtatta tcatgtttgt tttattcaag cacacttata atacgcttga
    12001 ttttttcaca atgatgaaat atactaatgg gtgttatagg tataagaatc gcattacact
    12061 tgaagaagta agtgataaat gagatataaa tcctcatatg ataaactact atttcattga
    12121 ggaacatatt ctaagtgatg tcaagatact acaatatgac taaattccta gtgataaaaa
    12181 agtctacgaa tgagtgtaga aaggggaact aagatgaata aagttttaat tatagatgat
    12241 gataaagatc tttgtatttt gttaaaaaag agtatttcaa ttgaacagat tgaagcaaac
    12301 tattgccatt ctgggagaga tggactggtt gaactttcaa aacaagatta tcagttagta
    12361 gttctagatg tgatgatgcc aggtatggat ggttttgaaa ctatggaaca aatacgaaaa
    12421 tatagcagta ttcctatctt aatgctgaca tcaaaaagtg ataattacag caaagtgcat
    12481 ggattgcgag ctggtgcaga tgactatcta accaaaccat ttgaaattac agaatttact
    12541 gctcgtgtac tgtctttaat ccgacgttac acccgtttta atccaaaaaa tgaggagcca
    12601 cagcaacttt ctttcaaagg tttgttgatt gaccttgacc agcatacagt tactaccaat
    12661 ggcgaaccta tgttgcttcc acccaaggaa tttggtgtac ttctttactg tgcaaggcat
    12721 cagggtaaga ttttgacaaa acagcagatt tatgaagccg tgtggggtga gccatatgta
    12781 tatgatgaca gcaatatcat ggctattatc agtcgcctaa gaaaaaaaat tgaactagac
    12841 ccatcatctc ccatctattt gcaaacaata aaaggaattg ggtatcaatt caacacggag
    12901 gtatgaaaat ggatgaacaa acgataattc ttttcttact cttgctgtcg attggtctac
    12961 tttttctttc catatttgct ttttccaagc ttgggcatat tcacaaacgc ttaaaagata
    13021 tagaggagat actcgctgat gtacaaattg gagacgaaaa tcgtaaaata ctaattaagc
    13081 catgtgatgt tatggctcca ttggtttatc agcttaatga gattgtctac gattatgaaa
    13141 ataagttact ttctctgaaa aagtcggata aagctagcaa acaacttatg acaagtttat
    13201 ctcatgatgt tcgtacacca ttaacaactc tgattggtta tctggatgct gtacacagtg
    13261 gtatagttat cgaacaagag cgggaggaat accttgaaat cgctaggcgt agagcctacg
    13321 acctcaagga ttacattgat gttctttttg actggtttcg gttgaactct gatgaattta
    13381 cgctgtcaat agaatctgtg gaaattgcag agttgtcacg caatattttg aaagactgga
    13441 ttcctatatt ccatgaaaag aaactgaatt ttgaaattga tattccagaa aatcgactaa
    13501 tggtaaacct cgacccagat ggatattccc gtgtggtcaa taatctggtt caaaatgttc
    13561 ttgctcacag taaggctcgg caaatcaaga ttaccatgag tgaggattcc agaatggtat
    13621 tactccgtgt tgaggatgat ggtgttggga ttgccagaga aaatttacca catatttttg
    13681 agcgactgta taagtgtgat aaaggacgtt cagaaaaagg aagtggtctt ggtctatcta
    13741 ttgtcagtca aatggtggag cgaatgggtg gtcaggtttc cgtagaaagt gagattggaa
    13801 agtataccgt atttacagtg agcttaccat taatataaaa ttgtttttcc cccatgtgac
    13861 atgtatacac agggggattt tttgtaagtt ataaagttgc aaggttaata caaggttgtt
    13921 gcaaggttaa tgtaagattg aggtggtatg ataactttaa taaacaaagg aggcttttat
    13981 tatttatgaa taatttaatt attgaaacaa aaaatctcac taagcaatat ggtgctcaaa
    14041 agagcgtttc taatttgaat attcacgttc agaaaggtcg catttacgga ttgctaggaa
    14101 gaaacggtgc gggtaagacg acaactatga aaatgttatt gggcctaaca aaaccaacta
    14161 ctggagaaat taaaatattt ggtcaggaca ttcacaataa cgagaagaag cttcttcctc
    14221 gaattggatg tttgattgaa tcaccagggt tttatcctaa tctgacagga acagaaaatc
    14281 tcaaaatctt tgcaaggctt agaggaatac caaaaaaaaa tgcagtagaa tcagcactgg
    14341 atgtagtcgg tttgccaaac aatgacaaaa agttattttc tcaatactct cttggtatga
    14401 aacagaggtt ggcgattgct cttgccatta tgcatgaccc acaactgttg attttggacg
    14461 agcctatcaa tggacttgat cctattggta ttgcagaagt aagaacgttt attcgtgatc
    14521 tctgcacaga acgaggtaaa actattttga tttccagtca cattctttct gaaattgcct
    14581 tgttggcaga tgacattggc attattgacc atggtatttt gttggaggaa gaaagtcttg
    14641 cccaattaga agaaaaaaac agtaaataca tacattttgc tgtatctgat gcagcacagg
    14701 cagcaaggct tctggaaacc caacttaacc tgaaaaattt caaagtagca gataattaca
    14761 atattatgct atatgataca gatgtttctg ttgcagaaat aaatcgtgct ttcattctaa
    14821 acaacattga ggtatcagaa tctcatttat gcgaagatac tcttgaagat tacttcaaga
    14881 aagtaacagg gggtgaagga attgcttgat ataatttcat gtgaattttc aaaactcaag
    14941 cggttaaaat ttatactgat ttctatttta ggtgcttgct tatttccaat tcccgcaaca
    15001 attttgattg caaaggacaa cttgcccttt gagcaattgt tcaaattagt tgtcaatttt
    15061 ggatatttct tgctattacc aattgtatta agtattgttg ctagccaact attttttata
    15121 gaaagagata atgatgtatt aaaaaatctt gcaacagtgc cagtgccaaa aggaaaactg
    15181 gctctggcaa aattggctgt gttgctgttt atatcattgt tctactctgt tgcagggtta
    15241 ggagctacta tcattggagg tttcattgtt ggaatagtag agggggttgc tgtaaaatta
    15301 ggaatgagca ttgcttttgg aattatgttt tttgttactg tacttcctgt tgtggtactc
    15361 attgtatatt tcaatcgcag ctatattttt tctattatac tttcttttgt atatgctatt
    15421 ttcaatttta gtatatcctt aaatattatc aattttgaac caaataaccc actattaagc
    15481 gtattacctg ctcctgttat aatgcgttgg tggatggcat tttggggaga tcccactggc
    15541 gaatacacgg tacttagaca gccatatttg ctctcaactc ctgcttgtgt aggtatttta
    15601 tgtttgataa ccattatagc agtcctgctg atttgtatga tatacaaaaa gcaggagaat
    15661 taggaggtat ctatgcttga tataattact acagaatttc aaaaaataaa aagatataat
    15721 attctatgga ttggtgttgt tgctgttctg ttttcagcac tattggcagt ttttcaggtt
    15781 gtaagttcac acggcagtga ccctctaacg tatgagagct ttgcaaacgg agttatctgg
    15841 aataatttca gtcttagttt tccatttggg attacactta ttggaggata tcttattaac
    15901 cgtgaatata cagaccaaac cttaaaaaac atcctaactg ttcctatttc attacgcaaa
    15961 ttgctagtgg gaaaattaat tgcggttgga ggaattactg tattgttaag tcttttcagc
    16021 ttcttatgca catttatttt ggggttgtta ttttgcaata tcaatatatc tgttatactc
    16081 gttgtaaaat ccttagttca gattttgtct gttaatctgt attgttatgt tgctgttttg
    16141 ccgattattg cttattttgg tcgtaaacaa aatgcatttc tgacgggtgt agggattgca
    16201 tttgtatacg gtttttgtgg tgtgtttgtt gctggaagaa atctgacaga cttttatcca
    16261 atcacagcag gtcttggtct agttggatac aacaatggtg ctggtactgt ttatcagcca
    16321 cttatcggtg ctatgacttt gattgtaatt ctaatcttga caactattct acttgtattt
    16381 acacctaatt atgataaagt tatgaccata tctaagaaaa aagggataaa gcataaaaag
    16441 tattctcata aataacataa atattgtttc tattatttaa gttgctagac agtgaaaaga
    16501 aaaaatctgt cgtacagtgg ctatatgctc atgtattcaa gaagctacag ttttgatttt
    16561 tcataactgt agcttctttt tgtatttatt aaatcacgac tatcataata cttgcaatag
    16621 gtatagtaac aagagagagt actgtcatat gtgttttata tattacatta agtatattct
    16681 aaaatatatg ttttaaataa tatatttgaa aataggaata tttaaataaa aaaaataata
    16741 ttatatatag ttgacaaaaa ttggcaaatg atatactata taaatataga aatagtaatc
    16801 attactatta ttaaaaaggg aggaattaat tatgaactta aaaggaacta aaacagaaaa
    16861 gaatttaatg gcaggatttg caggagagtc agaagcaaga aataaatata catactacgc
    16921 ttcaaaagca agaaaagaag gttataatca aatagctgct atatttgaag aaactgctaa
    16981 caatgaaaag gaacatgcta aattatggtt taaactttta catgatggta tgccttcaac
    17041 agaggaaaat ttaaaggatg cagcagcagg tgaaaactat gaatggactg atatgtatgc
    17101 aaaatttgct aaagaagcta gagaagaagg atttgacaag atagcttatt tatttgaagc
    17161 tgttggaaaa atagagaaag agcatgaaga aagatactta aagcttttag aaaacttaaa
    17221 tgaaggaaaa atatttaaaa gagatgaaga agttgtttgg caatgtcaaa actgtggtca
    17281 tgtgtatgta ggtactgaag caccagaaaa atgcccagta tgtgaccatc caaaagcata
    17341 ttttaatata aaagctgaaa attattaaaa attatcatta ttaaaaagga atgttaacta
    17401 tgaaattttc taaacaacga gaactgattt taaatgaaat attaaataat ccggttcatc
    17461 ctactgcgga ttacctatat gaaaacttaa aaaaagataa tccgaattta agcttaggaa
    17521 ctgtgtatag aaatttagct cagctaacgg aacatggctt tataagaaag gttagtattc
    17581 cagggtatcc agatagattt gatggtcgaa tagataatca ctatcatatc atatgtgaag
    17641 tatgtggaga ggtatacgat ttagaatccg aagttcttaa taatttacaa gaattaatat
    17701 ctgaggagac agacataaaa ataacatctt ataacataag ttttaaaggg atttgtaata
    17761 attgtaaaag gtgtagccaa gtaggttagt tttcaaattc taaaaataat taatgaggtg
    17821 agtttatgtg tagtgaacaa aaatttttta tatgtgagac ttgtggaaac cttgtaggaa
    17881 tgatacaaag tggtggtgta cctattttct gttgtggaaa acctatgaag gaacttgttc
    17941 ctaatacaac agatgcagca gttgaaaaac atgtacctgt aattgaagtg gatggaaata
    18001 atgttactgt taaagtaagt agcacaactc atccaatgac taaagaacat catatagcat
    18061 gggtgtatct tatgactgaa caaggtggtc aacgcaaatg tctagcagta gatggagaac
    18121 ctgtagttaa atttgctttg aatgatgatg ataaagtgat ttctgcctat gcttactgta
    18181 atttacatgg attatggaag gctgaactgt aatatttaaa taaattgagt caaaaaggag
    18241 gctataaatg tattttcata caaatagcct cctataaatt tatataaatt caagttttaa
    18301 ttttgtttaa tgtcaatata atataaggag aaaataatat gtcaatttat aaatgtagtg
    18361 tttgtggata tatttatgat gaatctaaga atgataaaac atgggatgaa ttaagtgaag
    18421 attgggaatg ccctgtatgt acaaaaggtc gtagttattt tggaaagata agtactgttt
    18481 actatgaaga agatgaaaag atagcagaag acatagttga agatgaatct aaattaaata
    18541 ctgaaaaaga gggagattta aattacttaa gtacatattt aagaagagat gatgaagttg
    18601 agaaacatat ggatataatt cacgaaatgg ctgttacagg aaaatctata atagaaccta
    18661 tgagaactaa actaccagta atttcttggg atgatatttt aattatgggt gctcaattaa
    18721 atccattacc tttaaatgag catgatgaag taaatacaac aacaataata ggaaaaaaag
    18781 caaagaaacc tatgattata gaaaatccag tttatatttc ccacatgtca tttggagctt
    18841 tgtctaaaga actaaaaata gctttagcaa agggtgcagc ccaaaataag acagctatgt
    18901 gtagtggtga aggaggtata ttaccagaag agaaggaagc ttcatacaag tatatttttg
    18961 agtatgtacc aaataaatat agtgtaactg aagaaaatct taaaaattct gatgctatag
    19021 agataaaaat tggtcaagga acaaaacctg gtatgggagg tcatcttcct ggcgaaaaag
    19081 taactgaaga aatagcaaaa gtaagaaata agcctgtagg tcaagatgta ataagtccat
    19141 cttgttttga ggaaatacaa tccaaagagg atttaaagaa acttgtagat gaacttagag
    19201 aagtttcaga agggcgtcca ataggtgtta aaatttcagc aggtcatatt gaaaaagata
    19261 tggaatttat agcttatgct aaacctgatt ttgtaactat tgatggtcgt ggtggagcta
    19321 ctggagcaag tccaaagttg cttaaagatg caacttcaat tccaacaata tttgctcttt
    19381 atcgtgctag aaaatacata gatacacatg gattagatat agatttggtt ataacaggtg
    19441 ggcttcgtat atctactgat tttgctaagg caattgctat gggggctgat gcagttgcta
    19501 ttgcaagttc tgctcttatg gcagcggcgt gtcaacaata tcgtatatgt ggttctggta
    19561 agtgtcctgt aggagtagct actcaggatg aggaattgag aaaaagatta catatagaaa
    19621 atagtgctaa tagagtggca aactttttaa atgtaagttt agaagaactt aagacttttg
    19681 ctagaatttc tggtcataaa gatatacatg atttaagtgt tgatgattta tatactgtta
    19741 actctgaaat atcaaattat acaaacatac agcatgtata gattgaagtt tgaaactatt
    19801 agattttaga cagctataaa agtaaatgat aaatatattt tataaaagag agtgtcttaa
    19861 aatgaacttt ttagttcata agtcactctt ttttgtatga agccaaatat atttatcatt
    19921 ttagcaatga aaaagaatct tatctcttta ttttgagata gactccttct atttatgaaa
    19981 attaaagata ttaaattgta aaatattctg tgatgtcgta ctattaaact aaagattctg
    20041 tactttaaag taaataaaaa tttaaggagt gattattatg gaaagattga agatacaagt
    20101 tttagtcgac aataatactt atattgatag atactttgtt ggagaaccag cagtaagcta
    20161 ttatattgag attgatggaa ataggatttt gtttgactca gggtattcag atgtattcat
    20221 atcaaacgct gaaaaattaa atatagattt aggaaattta actcatgttg ttttttctca
    20281 tgggcataat gaccatacaa gaggtatcca gtttttagaa aataggtatg atttatctac
    20341 agttgaactt atttcacatc ctaactgttt tatacctaga aaacatggag agaaaagcat
    20401 aggagctcca ttttctgctg aggaaattaa aaatattttt atgtataatc ctaaggataa
    20461 gccatttaat ctaagcaaga attgtgtgtt tttaggtgaa ataccttcta taaatgattt
    20521 tgaaaaaaga gctaaaattg gtaaatgtaa aataggagat ttgtgggaag atgattatgt
    20581 tttagatgat tctgctatag tttgtaaaac tgataaaggt atttttattg ttacaggatg
    20641 ttctcatagt ggaatttgta atatagtaga gtatgctaaa aaggtatgtg gagatgatag
    20701 agttattggt atactaggtg gttttcatct ttttgaattg aataataggt tagatagtac
    20761 aatacaatat ttggataaag aagaaataag aatgttgtac ccttgtcact gtgtttcttt
    20821 taaggtaaaa gcgaagatga atgaaatatt gaatataaat gaagttggag tagggctgac
    20881 cataagtata tagtatttga aagtatataa gatttaagtt gaaggttaaa aagatatttt
    20941 ctatagaaaa tat
//
LOCUS       DACULQ010000055        20928 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.055, whole genome shotgun sequence.
ACCESSION   DACULQ010000055 DACULQ010000000
VERSION     DACULQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20928
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.055"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(27..881)
                     /locus_tag="JA154_08240"
     CDS             complement(27..881)
                     /locus_tag="JA154_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HAT7995855.1"
                     /translation="MKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKEVP
                     SKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDIEE
                     VEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCDKI
                     IKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLNTI
                     KEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEEEE
                     EKYRRIFK"
     gene            complement(1204..2532)
                     /locus_tag="JA154_08245"
     CDS             complement(1204..2532)
                     /locus_tag="JA154_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HAT7995856.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2680..3009)
                     /locus_tag="JA154_08250"
     CDS             complement(2680..3009)
                     /locus_tag="JA154_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAT7995857.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3033..4337)
                     /locus_tag="JA154_08255"
     CDS             complement(3033..4337)
                     /locus_tag="JA154_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT7995858.1"
                     /translation="MGGIKMEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPII
                     IVGSLSMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKK
                     YNLNHVEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDK
                     NLVIRFPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAI
                     SSVDNVFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYV
                     FTIGFYYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPI
                     VMNPYMFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIV
                     LVFASIILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4338..4664)
                     /locus_tag="JA154_08260"
     CDS             complement(4338..4664)
                     /locus_tag="JA154_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT7995859.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4799..5428)
                     /locus_tag="JA154_08265"
     CDS             complement(4799..5428)
                     /locus_tag="JA154_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995860.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5644..8301)
                     /locus_tag="JA154_08270"
     CDS             complement(5644..8301)
                     /locus_tag="JA154_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HAT7995861.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKHYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8660..9604)
                     /locus_tag="JA154_08275"
     CDS             complement(8660..9604)
                     /locus_tag="JA154_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7995862.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9597..10622)
                     /locus_tag="JA154_08280"
     CDS             complement(9597..10622)
                     /locus_tag="JA154_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7995863.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10697..12265)
                     /locus_tag="JA154_08285"
     CDS             complement(10697..12265)
                     /locus_tag="JA154_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7995864.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGVFWNIADWTIE"
     gene            complement(12378..13289)
                     /locus_tag="JA154_08290"
     CDS             complement(12378..13289)
                     /locus_tag="JA154_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7995865.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13273..14259)
                     /locus_tag="JA154_08295"
     CDS             complement(13273..14259)
                     /locus_tag="JA154_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7995866.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14586..15020
                     /locus_tag="JA154_08300"
     CDS             14586..15020
                     /locus_tag="JA154_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7995867.1"
                     /translation="MKSNYDCFRIAMLLKELYSKTMYTVEENFKENGLTHQQIIVIKL
                     IAHNQELTISQLCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFE
                     FATNFKIKMQESFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15023..15421
                     /locus_tag="JA154_08305"
     CDS             15023..15421
                     /locus_tag="JA154_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HAT7995868.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(15871..16095)
                     /locus_tag="JA154_08310"
     CDS             complement(15871..16095)
                     /locus_tag="JA154_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HAT7995869.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(16130..17497)
                     /locus_tag="JA154_08315"
     CDS             complement(16130..17497)
                     /locus_tag="JA154_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7995870.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTAMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(17581..19116)
                     /locus_tag="JA154_08320"
     CDS             complement(17581..19116)
                     /locus_tag="JA154_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995871.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDAILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(19139..19573)
                     /locus_tag="JA154_08325"
     CDS             complement(19139..19573)
                     /locus_tag="JA154_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995872.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(19833..20753)
                     /locus_tag="JA154_08330"
     CDS             complement(19833..20753)
                     /locus_tag="JA154_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HAT7995873.1"
                     /translation="MIIMNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVF
                     TMYNPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVY
                     NRDIVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREE
                     SPKFFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIM
                     SGGFNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYI
                     PTNSKSQYCSPSCRNCSNVKKSRSRAKKDK"
BASE COUNT         6579 a         3733 c         2175 g         8441 t
ORIGIN      
        1 tatttttagg attctgttac ataatattat ttaaatattc ttctatattt ctcttcttct
       61 tcttcagtaa acacattaaa tacaactttt aaattttttt caggattttc ttttagccat
      121 agtctaactc tacttacagc aagattagct gcctcttttt ttggataacc aaatacacct
      181 gtagaaattc cacaaaaaac aatatttttt atttcatcaa tttcttttat tgtatttaaa
      241 catgatttat aacaatgaag cagttgcttt tcttgctctt tacttggctg accaccagat
      301 acaataggtc caacagtatg tactacaaat tttgcaggta aacaataacc tctagttatc
      361 ttagcatctc cagtatattc taaatgccct tgttttttta ttattttgtc acaatcttct
      421 ctaagtctag gacctgcgca tgaatgtatc tcattatcca cgcatagatg taatggttgt
      481 aaacacccta ataacttgtt atttgcagca tttactattg catctgctct taaattcgtt
      541 atattacctc tccatatggc aatcccttcc tctacttctt ctatatcatt tacatctact
      601 aactctcttt cttcacattc aatcaacaat aaattatgta acatattaag tatctctgga
      661 gcaatttctt ttggtggata tacattcaaa agtcctctaa gtatatctct aggcttaatt
      721 tcatcatttg ataaattgta tattttagaa ggaacttctt ttgaaaaata tgcaattaaa
      781 gtgtttatat tttttcttct ttgttcgtca gttagaggct tgatagtttt atcaaaatcc
      841 tcaaataaat ttacatcatc agcataatct ctccacttca tagccatctc tccattcttg
      901 tattttagac ttaaaaataa ttatgtattt atgtacatat ttatttattt ctataaacac
      961 ctacataaat cctgttaaaa gtgtaaattt tttattatac aacttctctg gttatttcta
     1021 ttgattgata gtaaattatt gtacctaaat ttaatccaac taacaagtaa ctaaacctac
     1081 tttcctaact gcaaaataca ctatgtttgc cacttgtatc tcatttgtag ataaataaag
     1141 tccctacaaa tacattaact aggctatgta ctaaactgta ggaactttaa aaatatatta
     1201 ttcttaatta agttttggcc aataattttt atttgcttct attaaatcat ctagtaattc
     1261 ttttgcaact gttgcactag gaactgtctt agatagcata agtgcttgcc acaatttatt
     1321 ataagacttc tcaatccaag catcaactac taatttttca actgctaatt gttgttctat
     1381 taaacctttt tggaatgttg gtattttccc tattgataat ggttcatacc cgtcttttcc
     1441 aactatacat ggaatttcaa ccatagcatc atcttgtata ttttctatag ctccattatt
     1501 ttgaactatc aataacatac gctcctgtgt attaaaagct agagctgtag ctaaatccac
     1561 tataaactct gcatgtattc ctatcttaag cccactgccc ttagaatgtc cttgcttttc
     1621 tatcaatgca catgaaccaa aaacttcttt ttctcttcca tcaataactt cattggcacg
     1681 agtataattt acatcagaat gatttacaac ataatctgga aatagatagt actttaagta
     1741 actacttggt attgtagtta aatctgtttt tactatttct ttagtaaata aattagtttc
     1801 aaaccaacta tctttcttgt catttttcat tccttcttca ccatttataa aaccatattc
     1861 ttttatatgt tctactaatt ttggtaaaag ttcatttcct tttttatctt taattgaagt
     1921 ataccaacca aagtgattta atccaaagta tcttatatcc atatgtttac gagatttgag
     1981 tcctgcaatc cttgctatat tatgttccat acctattggc atatcacaaa tatttattac
     2041 cctagaatta ggtctcaacc ttctaacagc ttccgctatt attgatgctg gattagaata
     2101 atttaacatc caacaatttg gagaatactt ttccatataa tctatgtttt caagaacacc
     2161 ttctatagac ctcattccat aagctattcc acctggtcca caagtttctt gaccaactac
     2221 cccatgtttt agaggtatct tttcatctag ttctctcatt tcaagttttc caactcttat
     2281 atgagctaaa caaaaatcta catctgtata agcctcttta gggcatgttg ttgctaaata
     2341 ctctatatta gggtcctttt cctttattag tatttcacaa gctttagcta ccttattttg
     2401 tctttcttca tcattatcat ataatttgat tgctcttaat ggtaacctat ctaagttatc
     2461 tagcaacatt aatatgattt ctggggtata tgtacttcct cctccagcta ttactactga
     2521 aaatttttcc ataataatat ctccttttct atttaaaaat ttactgtatt tataaataaa
     2581 tttataaata ctagtacttt aaagtaaaag ggagttcatt tattaaatct attgatttaa
     2641 taaactatag ccaaacttca agcactattt acaagaaatt taattcttat taattcttgg
     2701 gtccatcatc tcaaatatat ctacaagttc agatactaaa gaatgagcaa ataatgcatt
     2761 tgaataatgg tcttcagagt gcacaactaa aaaatcagtc ccactatctt cactattcat
     2821 agcatttgat agtaattcta aatgatgctt tgatgctgtt tgtattttct ctgaggctga
     2881 atttaaaagg tgttttgctt tattgtagtc accttttttt atttccttaa gagacatcat
     2941 tacatcactt ttagcatctc ctgcttctga tattatacta aatacttttt cttccatttc
     3001 aggtaccata tccttctcct tctaatgact tgttaaaaat caaagctatc atcatctaat
     3061 gttgtagcta attctgcatt tttatttaat tcttccttct ccattatctt aaagaatgga
     3121 taatatatca aggtcataag tattatactt gcaaatacta atacgattgc cttccaatct
     3181 aatgtcatca aaaatgctgc aacccctgga ggtaaaaagc ctggtggttc tgcaataggt
     3241 aatcctacta atcctacttt taatgccata aaattaagtg ctccaaccaa tacaggtcca
     3301 aatacaaatg gtacaaacat atatgggttc ataactattg gtgttccaaa tattaccggc
     3361 tcatttattc caaatagtga tggtaaaatc acaacttttc ccatactatt aaaactctta
     3421 cacttacttc ttgacataag gtacactaat cccaaagtta ttcctgaacc agttacttgc
     3481 aagaaaccat agtaaaatcc tattgtaaac acatattttg ggtctcctcc tgctgctatc
     3541 agtgttgcat tttctgccat atatgtagct gctattggca tccatatact tgaagttatt
     3601 gctgctccat gaagcccaaa gaaccataat aattgagtta taaatagtat tatagttact
     3661 gcaaaaacat tatctacact gcttatagct ggtgctaaga aattcattat aatagctggt
     3721 agtggttgac ctgagacatt tttagttatt gctgctattg ctgtaaataa cattatacat
     3781 actatcattg gagttaaatt tctaaaactt ttatttacta cactaggtac ttcttttgga
     3841 aatctaatta cgagattttt atcattcata aacttagtaa tatcaactac taaaagtgct
     3901 actaatatac ttacaaataa accttgtgca tctaaataac ttgtatctaa tacaccatct
     3961 actacttgtc cacatacaac aagttgtgca acaactgctg tcatgcctat ttctacatga
     4021 ttaagattat attttttagc tagtgcctga gctataaaaa atgctgaata tatagataat
     4081 aaactcatag taaatttatt agggataagt aatgtatcca tattttcaga taataatgtt
     4141 tttgcacttc cttcaggtaa catattgttt aatgccataa ataacatact taaagaacca
     4201 acaattatta ttggggtaat gcttatcata ccttctttta tagcttgaag atgcctttgt
     4261 ttttcaatct tttgtgctat tggaagaaga aatcttctta aattattttg aagtgcttcc
     4321 atttttattc cccccaatta tctattttta tataattcga ttgccgactt cataacagaa
     4381 ggagcatcca tacgaccata atgcttagta tctattaaac caactgctat tttgtaattc
     4441 ttacaagctt caactacatc atcttcctta aacctaattt gtggtgctac aagtactaca
     4501 tctgtatcat tagcttcaat gtatgatttt aaattttcaa atgatgttgc ctctatattt
     4561 atagttttag tttttaactt ttcatgctca ctacaaaaac ttttcatctt ttccacaagt
     4621 acacctgttg aagctccatt tgcacatact aataaaatat tcataattac cctcctacaa
     4681 tatctaacct cttttatttt ctaatttttt atcagccaat tcttactcta attcctatca
     4741 gtttttactt taattcttat tattttattt taattcttat taatatttac tctaattctt
     4801 agtactgcca atgattaaac ttgaaagccg tatctataac ttcttcttgc ttatattttt
     4861 ctaatatttt taagtatctt tctttataat cattatctac ttctttttct ggtattacta
     4921 tactagcttc atttaaaaag tggaatgaat cccttcccat ttctaatcct ctatatgtat
     4981 gcttgtcaag tgccacgtcc ataacttctg gaactttacc cattgcaaat ccttttataa
     5041 ttatattttt taataaatca cttgatctat ctttctcaca tgcacaaaga tatcttattg
     5101 catgtatgaa aaataaaggt ctgtctccat ctgcatatga atattccttt cttacttgat
     5161 ttaaattatt tatgattatt acagctgatg gatcccccat tcctatatct tcaactgata
     5221 ttgtaagtat ccttctccat agcttatctt ctaattctgg tgatgatata tatagttcat
     5281 aagcaaattc acaagcttct tccaccatat ttcttcttat agctttttga aatgacgata
     5341 ttatttcgtc tgcactaaat ccgtttcttg tagtcattct tgcccatggg tcatttgcct
     5401 ttttatctct taatatttta tcttgcatat tatttactcc tttttattta aattttaatt
     5461 atttatatta ctcatttaat aatataagca agtattatgc cagttttttt agacacttta
     5521 aaacactgtt atttcaatca tttttatcat caaaacttag atactttgtc taagtatatt
     5581 tatatgtgtc taatattaat attattttag actaaaaaaa aattaccaaa taagttttta
     5641 tactcattgg taatcttttt cgattatttc tgcatattct aatattaata atatttgata
     5701 gcactctttt tcattaattt ctatattata ttttttatct aattgagata ctatatcttt
     5761 tattttgctt agactcttgt gataatcaag tgttttttct ttatcaagct tttcaatttc
     5821 tctagtattt ccaatcaacc catctaccat gaaagctaaa tgcatcataa gtccaattac
     5881 tttatcgtat ttcttttcta tattaaatac taaaaacaat tcatcagaca catattttat
     5941 atctttaaat attctattat aattagagct ttttaaggat gtctttaaac cttcaactac
     6001 atcatcataa cctatactta tctttataat attttttaat ttattgatat tttcatcctt
     6061 atctatatct tttttgctaa tatagtcaat tcctctaata ttaattcttt gttcattgat
     6121 tactgcaaca atattactac tttctttaag tttcgtaata tttaaactta aatctttttc
     6181 tgttttatat gggacagata taatatctat tccatctaag tacctactca tcttttcttc
     6241 taatattttt cttgttttat tatctacacc ttctgaaaca tgtgatgcta taataattgt
     6301 tttagacaga ttttctttat tttgaatttc attagttcca aaatagtgct ttctcgtgtt
     6361 agtgatagag ttgtatacgt catttaaagg taatcccata ttggccttct gaactgcttc
     6421 taacacagtt aatgtcgtca ccataggaat tactcttact ccacactttt tttctttctt
     6481 tataatttca tcaaaggagt ttaatgaacc catatctgta aatagtaata catctttatt
     6541 gtctatatta ttaattaaat ttttaaaatc tacaataact tcatcatagc ttttatctaa
     6601 cttcatatcg aaagaaatag cactgcactt tccaataata tcaccaactg cttttaccat
     6661 actacttgct gtagtttctc catgcatagc aactataata ttaatgttat tcactatttt
     6721 attttgttca tcttgtttag cttgtactat gaataatgta atatatgcta cagtttctat
     6781 agataaaaat aaatcaaatt cattttcaat tattccaact atttttaatg ctgttttaaa
     6841 ttcacttctg ttatttcttc taatttgatt caaatctatg ttgtttgtag agctactttt
     6901 atccaatacg ctttttatat aaattgccat tgacttaaat attgattcac ttatgtctat
     6961 ttcttcatct tctttaataa tttgtcttat tgattcacac atgcttttaa acttaacatt
     7021 attaaataga gaagcatctg ctatattcat ttctacagat ttattaagat tagtatttac
     7081 tgcattaata atatttatat cattgtagtt aataacttct tcttctccta atgacgtaaa
     7141 tacaaaatat tctattccat gtgttataac ttcatctgct ttaatagact tctctctata
     7201 atcatcactc acttcattta tagatttatc aaaatatttt ttctctatat aaactggttc
     7261 tttattatct cttttatatc ttagatatcc atttgcaaca gctaatttta tatcattttg
     7321 aagttgacct acattatttt tacaatcata aagtaatagt gatttaatta tttctttatg
     7381 tacttttatt gaaatagaaa tactttttga ttcttccttg aaaaaatttt tgactaattc
     7441 cattctttca atcattgtcc tatctctcaa tgatggtatt tctattttca ttggtattct
     7501 tctattgaat gtatctaata gtgttgatgt cggattttct gtagtagcac atattattaa
     7561 taccttagac tctctttctt tcgttgcatc tcctagtctt ttaaatgttc ccttatctat
     7621 aaaagtaaac aacatttctt gaccttctgc tggtagtcta tgaatttcat ctaaaaatag
     7681 tatccctcca tttgcaattt ccaaaagacc ttttctggaa gattctgcac ctgtataagc
     7741 tccttgttct accccaaaaa tatgtcccat aagtaattgt gcattattag cataatcagc
     7801 acaattaaat gttacaaaag gagctttatt ttttctaatt ccactatcta tagaataatc
     7861 gtacatatat ctagcaatca tactctttcc tactcctgtt tctccataga taatagtatg
     7921 aagtccattt gtaggataga gcattgcgac cttgcatttt tctattatag gtcttaaact
     7981 atgttcttta cctattagtg tttcaaaact atcttttttt attttgtttt ctacttttgt
     8041 tccttttttt atgccaagca actcactttg tgtatcaagc aattcattct tgttaaggtt
     8101 aagatttgca gggatataat acacaggtct tccttttatc ttcgtaacca taccctcatt
     8161 gaataattgg tttaaatatg tacttatatt gtttcttgca attcctgtct tttctgataa
     8221 ttggttagct gtaatccctt tttcattttc cctatatgat gattctaaaa ctttcattac
     8281 tttatctttt ttatatatca ttacaagctc ccctacatta tagtatttta ttaactaaaa
     8341 cccctataca cactttattt tttaataaac tcttattctt taactaaggt tataatttat
     8401 caaactttcc ttactttaaa ttatagaaaa ttattgtaga aaaaataaga ttattgctca
     8461 ttttttctac attactattt ttaattttat agtactttct taacaacata ctttacttgt
     8521 ttttaatttt attatatttg aatttattat atttgaaaag tagaaataaa aaaccctatt
     8581 ttggagtagt tttaaaattt ctccaaaaat agggttttag tttgtatttc aaataaatac
     8641 ctgttcaagt atttttatac taaacaactt ttaaattttg ttgatattta tttatttctg
     8701 atttagaaga atatacaaaa tgtccatctg aaacctcaat ccaacttaat gacttttcat
     8761 ttgaacaatc atatccatta gaattatact ctattctatt tcttgactta gcatatctag
     8821 ggtcaggaac aggaattgca gaaagtagtg attttgtata tggatgaata ggattattat
     8881 acaattcatt tgaatctgcc aattctacta attttccatt atacataact cctatacggt
     8941 cactaatatg tctcaccata gataaatcat gagctataaa taaacaagtt aaattctttt
     9001 cttgttgcaa ttttttaaat aaatttacta tttgtgcttg tatagacaca tccaaagaag
     9061 ctataggctc atcagctatt ataaattctg ggtctactga aagagctcgt gctatgccaa
     9121 tacgctgtct ttgtccacct gaaaaatcag atggatactt atttgcataa cttctatcaa
     9181 gtcctactag ctttagcatc tcatatactt tattcattct atctgtttta tttttacaga
     9241 taccttgaat ttttagtggc tctgaaatta tatctgcaat tgtcatacgt ggatttaatg
     9301 atgaagtaga atcttgaaaa ataatctgca tacttttgtt tacatctttt tttataaatt
     9361 tgtacgtttt tttatctgat atgcaatttc ctttgtaaat tacttctcca ccagtagatt
     9421 catgtagtct tattatagtt ttaccagttg tagattttcc tgaacccgac tctcctacca
     9481 atccaaatat ctcaccctta tatatatcaa aacttatatc atcaactgct tttacagtag
     9541 tacttttgtc caaatgaaaa tattgcttta agttttttat ttccagtatt ttttctctat
     9601 tcattagaaa tcacccttcc acaatttact ctaactggta catctacttc aggtgcatct
     9661 gggtgcaata accaagttgc tgcactatgt gtatcattaa ttttaaacat tggtggctct
     9721 ttttcataat ctatcttaag tgcattttta ttccttattg caaaagcatc tccttttggt
     9781 gggttcaata aatttggagg cattccaggt atattatata aatagtcatc ctgcgaatct
     9841 aaagtaggta atgaacccaa caacccccaa gtatatggat gtctagggtc ataaaatata
     9901 tcctctacag ttcctatttc tacaatcttt cctgcataca taactgctat tctatcagct
     9961 atagttgcaa ctactcctaa atcatgagtt ataaatatta ttgacaaacc tattttatgt
    10021 tgtaaatcct ttattaaatc aattatttga gcttgtatag ttacatctaa agcagttgta
    10081 ggttcatcag caatcaatac atctggatta caagcaagtg ctatagctat tactatacgt
    10141 tgtctcattc cacctgaaaa atgatgtgga aattgtttaa atcttttctc aggattatca
    10201 attccaacca attcaataag ctctatggct ctcttttttg cctcactttt acttatacct
    10261 tgatgaatga ttacagcttc tgcaatttgc tttcctattg atatagttgg attgagagat
    10321 gtcatagggt cttgaaatat cattgatata ttctttcctc ttatatcttc catctctttt
    10381 tcagacttct taaccaaatc acttgattta agtaaaacct ctccattttt aatatatcca
    10441 ttgtatggga gtatcctcat caaactctta cacagtacag atttcccaca accagattct
    10501 ccaactatag ccaatgtctc accttttttt agattaaaag atacatttct tacagcctga
    10561 acttctcctt cttctacttt aaaacttaca gatagattat taacctccaa taaatgttcc
    10621 aaaaaccatc aacccctatg ctattcaaac cttagggata tatttttatc cctaaggttt
    10681 ctaattaatt tcagtattat tcaattgtcc agtcagctat gttccagaat acacctacac
    10741 catgatgacc taaaactgta tcaggtgtta atccttttat gtttggtttt ccaacatata
    10801 ttgcatctat ataagcaata aatgtataag gcatatcctt agtcatttct acttggaatt
    10861 gtttatataa ctttaatttt tcatctttat cttctgtttc tcttgctttt tgaagtattt
    10921 tatctatagt aggattagag taagcactgt aatttgcacc tttatctgtt ccaaatactt
    10981 tatatgtatg gtcatctggg tcaaatggac ttccccaacc tattaaatga gcatcttgat
    11041 tagcccaatc tgtttcagtt acaacaacag ctttagcatc aacaccaatt tcttttaact
    11101 gttgtgcaca tattttagcc atatcaactc taacttggtc actctcacct gctgttatct
    11161 cgaaagctaa cttagtacct tctttttcat agattccatc agaacctaat ttccaaccta
    11221 gcttttcaat ttcttgttta gctttttctg gattatattc aaacttttct atatcaggat
    11281 tattatatgg tcccatttgt aaaggtgaat atgctggtac accatgtcct aataatacac
    11341 tgtcaactat tgcttttcta tctattgcat agcttaaagc atttggtaat cctttagctt
    11401 ttttatcttt aaagaattta gaattaaaat tgtataatat acctctatag tcggctgttt
    11461 tcattatatt tactttaaaa ttcttttcgt ctttttcaaa gttagacata tctttaggag
    11521 ttatttgagc taggtctaat tctcctgatt ttaattgcat tgccttagcc ttatcatctg
    11581 gaacaatctt aaagactact ttatctaatc ctggttcttt tacaaaataa tcactattct
    11641 taacaagtgt tatactttga cctttgtccc atttttcaag tttaaatggt ccagtaccta
    11701 ttggtttttg attaaattca tcagtagcta tatccttacc ctccaaagca tgttttggta
    11761 atactcctac tgttaaataa tcaagcattg ctgtatttgg agcttttaaa gtaattttta
    11821 tagtattatc attaaccaca tcaatttttg ttatatcttc ataatttgat gctatctcag
    11881 aagcattatc tggattcatt atagtttcta ttgtaaactt aacatcattt gctgtaaatt
    11941 tctctccatc atgccactta acatcatctc ttaaattaaa agtatatgta tttgttgcct
    12001 catcaaattt ccagtccttt gctagacaag gaaccacctt attattttca tcatgagctg
    12061 ttaatccatt aaatattaac gaatttattt ctccatgttc atatagtgca ggatttatac
    12121 ttgtatagtc attacttcca taaacaagca cctttccatc tttaggcgta tctgccttct
    12181 tatctttgtc tcctccacta gaacaacctg ccattagact aagtatcatt actaaactta
    12241 aaacttttaa cttctttaat ttcatgatgc tccccctttt gctttttata aaatattttt
    12301 caaagctttg accccattta tttaaataga tatttcaaat acctcagtat ttgcttattt
    12361 aatactcaaa tgctgattta taaattacta gattttttat tattattctt tcttatataa
    12421 ttacctatat ttgttataca caccaatgtg gtaaccaaaa atatacccgg aatcagtata
    12481 atccaccatc tatttgaaag taatgcttcc tctgacaaag ataacatact tccccaagaa
    12541 acaatttcta ttggtaaacc aattcctaaa aagctaagtg tcgattcagt accaatagct
    12601 gcgcctatgt ttgttacaac catgaacatt attgatgcca caaaatttgg aagtaaatgt
    12661 tgctttaata catagaagaa tcctcctccc atgctcttag ctgctagtat gtattcgcta
    12721 ttttttattt gtctaacttc tgttctcact atcttagata tattcatcca acttgttatc
    12781 cctattacta tggacataga tactggattt gaatttccaa gtatagcttg tacaaatatt
    12841 ataattaata ttgaaggtat actaagtatg atttctgtaa acctcatcat tgcatcatct
    12901 acgagttcag atgccgaacc acttatacta ccataaacaa ttcctataac agttgatatt
    12961 acagtggaaa atagtcctat aaataaagat atttttcctc cataccatat catagaatat
    13021 atatctctac ccattgagtc tgttccaaat aaaaaagttt tatttggagc taaattagaa
    13081 cttgctaaat ccatgtatgt tggttcatgt gtcattataa aagatgagaa tacacttcca
    13141 atgactatga ttgataaaat aataattgat atatatggta attctttaat ttttttagat
    13201 aaggttttct ttgtttctat atgttcatct tctgctacta aaatataatt ttctcctaca
    13261 atttcaaaat cactatattg tggcttcatt agtatcacct ctatcatatt tcattctagg
    13321 gtctatttta ttgtttataa tttgagctaa catattccca aatactacta aagcccctgt
    13381 tattaagcat aaaactaata acatattata atcatgatac tttgcacttt caaaacaaag
    13441 tgttcctaac ccaggataag aaaaaacttt ttcaactaca taagttcctc ctagtatatg
    13501 aggtatcgaa atggccatta tactaatata tgatggcatt atatttctca ggcaatgctt
    13561 aaaaacaata gttctattat ttaaaccttt tgctttacat aaaagcacat aatcttctct
    13621 tatttcctct agtaatttat ttcttatcat atatgtgtaa taccatagat ggcttaatat
    13681 aagtactgta agaggtaaga ttagatgtga tatcctactt aatatactgg actcttctcc
    13741 cattgaataa gcaccactac ttggaagtat acttaagttt atactaaata taagtatgag
    13801 tactaaggca acccaaaatg atggaataca gttggttatt gttcctaatt tgcatatgat
    13861 tctatcaatg agcttatctt catgtaatgt acaaaatatt cctaacaata aggctagcac
    13921 aaaggtaagt atatatcctg aaccacctaa tattatagtg tttatataga catcatttat
    13981 aacagaagta acattttgtt tatatttaaa tgaaattcca aattcccctt ttgatgcatt
    14041 tgaaatccat tttatatatt gactgtatat tggttcatcc aaaccaagtt ttttcatagc
    14101 attttctttt tcttgtgtac tcattctttc tactccatcc ccatagtaag agattaatgg
    14161 gtctcctgga gcaagtcttg ccatgtaaaa aacaataaaa gataataaaa acattactaa
    14221 aataaactgc aacaactttt tcagtatatc ctttaacaaa ccccaacccc cctttgttgc
    14281 aataaaaaaa accacataaa agcaaacgta aagtaacgtt aaaaccttcg tggttccatt
    14341 atcattaaac aaaattaaat tttctatatt tatctatgag ttaattttag catcatttat
    14401 taactgtgta aacaaacttt ttaaaaattg ttaaaatttc atcaaattat tttatttata
    14461 cctaaaataa aatttttata ttctattctt cattattttc tataaaatta taaaatttta
    14521 ggttgactat ataagaaaag tatattatat tagtttgtat gcaaaccatt taagaggtga
    14581 ttttattgaa aagtaattat gactgtttta gaatagccat gctcttaaaa gaactttatt
    14641 ctaaaactat gtacacagta gaagaaaatt ttaaagaaaa tggattaaca caccaacaga
    14701 ttatagttat aaaactgata gctcataatc aagaacttac aatatcacaa ctatgtgatg
    14761 aaatgtcttt agctaaaggt acggtttcag gcataatcag cagattagaa cagattggat
    14821 atatagaaaa attcaagaaa tctaatgata aaagaaatac atacgttaag tttacaacaa
    14881 caggatttga atttgccaca aattttaaaa ttaaaatgca agaaagtttt gatgatattt
    14941 ttaaaaattg tgatgaaaac gaattgagtg acttagtaaa aaatcttaga aatatattgg
    15001 caaaagtaaa ggagagataa ttatgaatta taaattaata cttgcaatag tatttataac
    15061 atcagcttta attttctaca ctataggtgt atttggagaa agaaaggcaa aaattttaaa
    15121 aaagaaacat gtaattatat tttggcttgg ttttatattt gatactttag gaacatttac
    15181 aatgagtaac atagccaaca gccatacttt tgaagtaaaa tctgctttat cacaaaacct
    15241 tcatagtatt acaggtcttc ttgcaattgt gcttatgctt tttcatgcaa gttgggctac
    15301 ttttgtccta tacaaagatg atgaggaaaa gaaaaaattc tttcataagt ttagtatagt
    15361 tgtatggact atatggttag ttccatattt tataggtatg tttattggta tggctggata
    15421 gtaaaataaa aagtgtgtaa gaaatactgt aggtaaattt gtataagaca aatttatcta
    15481 catccttctt tcacactttt acatatttat ttataatact ccattatttc tttcatgttt
    15541 cttaatacct atatagaata tttaattatt tctatagtat tttattattt tctcacactt
    15601 tttcatatat atattgtttt actatttctt catatgtata tatgaaatat tccattattt
    15661 ctataaatgt tttattatct ttctcatact ttttcatata tatattgctt cactattttt
    15721 tcttatttct tcatatacat atgaaatatt tcattatttc tataatgttt tattatcttt
    15781 ctcatacttt ttcatatata ttgcttcact atttttttta cttctttatt aaatacttat
    15841 gtataacata catttaataa acgctaacta ttaaacacct aatacttgag ttactcctgg
    15901 aacttcactc accaaaacat tttcaataat agctttgatt gtcatagtcg ctcctggaca
    15961 cccactacat gccccttgta gtctaactaa aaccacacca ttttcattta catctataag
    16021 ctctacatct ccaccatctc tttgtaatac tggttttatc ttttcctcaa gcactttttc
    16081 aactttttct ctcatagcta ttccccccag aaatatttta ttagcaaaat tataccaatg
    16141 gtttaaattt ttgtccatct aatgatttat caaattcaat ttttgcttca tcaataattt
    16201 tagtatccat aaatacatca taagcactac aagccagtac tttagctgaa tttatcatag
    16261 cagaaaatcc tatatttgaa cctgctgatg aacatgactg ccatgtatga cttgcaattc
    16321 caacaggcca agctgccatt gcaaattgag ccactggtat tacataactt acatctccaa
    16381 catcagtaga accagctagt cctttatctt tgtcaccgtc aactatttcc ttgtgtatat
    16441 agctttctac aatagattta tcattttgta atgatgttgc tacacctaat ctttcttctc
    16501 tagtaagagt atcacacaac ttttttgcaa attcttcttc ttcaggagta tttttagggc
    16561 aaccaacaaa aaccatattg tcataaacta aatcagtcaa acattggttt ggtatatagt
    16621 catatattcc atctgtaacc ttatactcca tttcagtttc tgtcatcatt gctgctcctt
    16681 gagctacttt tattaatcta tcaagaacgt gttctgcatc ctttgctttt ttacctctaa
    16741 taaagtacca actttcagca aatccaggaa ctacattagg tcttccacca ccattagtta
    16801 taacatagtg cattcttata gagtcaataa catgttctct caaataatta gctccaacat
    16861 tcataatttc aacagcatca agtgcgcttc taccattatg aggagcttga gctgcatggg
    16921 ctgttatccc tttaaattta aattttacag ataagtttgc cagacttcca cctctccaag
    16981 gtgcatttat atcaaaagga tgccatgtaa atgcacaatc tacatcatca aaacagccat
    17041 ttatacacat agctgtcttt cctccacctt cttcttcagc aggacaacca aaatatttta
    17101 ttgttccatt aaatttattt tcttctatta atttttttat agaaacaact gctcctacac
    17161 ctgctacacc caacaggttg tgcccacatc catgtccagc ttttccttct accaatggct
    17221 ttctttctgc acttacacat tgtgatagct ctggtaaagc atcatattct ccaagtatgc
    17281 caatcacagg tttaccctct ccaaaacttg ctataaatcc agtaggtaaa ttttctactt
    17341 cttcaatttt aaatcctttt gactttaggt attctttttg aatgctactt gcatactttt
    17401 cttcaaaggc taattctgga ttttcccata ttttcttaga taaatcaatt aattcttctc
    17461 ttatttcttc aacattattt atgatatact ctttcataat tttcctccaa atatatgtat
    17521 ttttgatatg ctttacagat tatattatat actaacattc tatttattca caattttttg
    17581 ttaattaatt ttattcatat gctcttctat ggagtatatc ttatcttcac tttgcctctt
    17641 cataaattta atgtactcta tcttccataa agtagtttca gttttctcat ttaacataaa
    17701 ttctaatata ttaatttctc tcattctaat atcttcataa aatactacac tacccaatat
    17761 tgccaattct aattcactta atgatgcaaa caacattaaa tactcttctc caaataaaca
    17821 ttcagattca agaatatcga taaagtcttt tttagcattg atattttcct ttattattct
    17881 tattttttca attatagatg tactatctaa tatctcattg aatatctctc taaacgattc
    17941 atcatccaat ttactttcat catcaacaat ataatcttct aagtgttcct ttttattttt
    18001 actcacagtt accatacttt taaaagtgtt actttttatt gagcttagca cagattcaat
    18061 aaatatcttt tcatattttt taatatatct tattacatct tcatcttcta tgcttaatct
    18121 ttttattaat acttcaattg cttccataag tagtaaagtt atttttttat ttattctaat
    18181 ttctctgtta atttcttcaa aatcattata atccaaatta ctatctactt cagtgttaaa
    18241 actatcatct acttcaatca attcaattat attactttct tcagagtcta taactttatc
    18301 ttcagtaata ttattgtaca tatttttaaa ttgtttttct aaaaattcat actcatattt
    18361 tgaaatttct aaattagtca cttcatttcc taaaatagta gaaaaaatac aattatttat
    18421 aataagttca aataaattta caagtatagc atcacaatct attttataag tcaccccata
    18481 gtcataaaat aatttttctc tatctttttt cttaaatagg ttacaaaatc tattttctat
    18541 atctatactc tcaatataat tcttcacata ataaataccc ttaacatcca tatcatctag
    18601 tgccaatgga taatctatac ttactggaac gtcctgagct tcaaatttta caatataact
    18661 tttaaagaaa cttgataact ccatcaaggt atcattatat gctattgaat tagtatccaa
    18721 ttttctactc atcattagtt tataaaaatc ttttagccta actaactctt cctctaatat
    18781 ttcaatgcct ccaaggtaca cttttttaac attcttagca gtcaactcca caactctttt
    18841 ttctatatcc ccaagcttat ttatatatgc atcaatagca taccatattg aaatcaacag
    18901 tttttcggca acttctgttt ttactgatgt gctttgacca tttgtatatt ttttaatcaa
    18961 atccataagt attagagata tttcttgttg tatattatat acttctcttt catctatcac
    19021 tttacaattt agacattcct taagtagaga tatggaatat tggttcttta ttaaattatt
    19081 ttcgtttata ttaatattac ttaaattatt taacatacaa aatctctcct atcatatatt
    19141 aaacttctcc aaatttaaat ttctttgcaa attcgtctgc ttctcctctt acattgtcca
    19201 tattgcctga aaatttttca taaaattcac ccagtatttc tataatctca tcatcacata
    19261 tcttgtcatc agtttcattt tttaaataat aaaaaatttc ttgcatatca ttaatagcct
    19321 ctaaataatt gtccacattt gtatattgtg atgtatatat attttctatt aactgttttg
    19381 taacttttat atctaattct attcttccat acccctttaa agtattatct ctcgaattta
    19441 ttatatcttt tacatcagat tcagaaagag ctagaccata atctccagat tctatattag
    19501 tttcaagcag ttcatttctt tctatttcct ttaaattctg atttatagaa ctcaaaataa
    19561 taggtgatat catttttaaa ttctcctttt ttattttttc cattgacttt ttttgttttt
    19621 tatgctatac ttaaattaat ttatattatt ttaccaacca cttataatag tagtgttaaa
    19681 agtggttttt gtcaactcaa aattactatc tcaattaaac ttttcaattt tctcaacaaa
    19741 aaaataccat catttagata tataactttc tataacaact tttaattcag tagttattta
    19801 ctaaaatcta aacaatgata tatttattaa ttttatttat cttttttggc tctacttcta
    19861 cttttcttta catttgagca atttctacaa gaaggtgaac agtattgaga tttagaattt
    19921 gtaggaatat aaattttacc acagtacttg catttattta aagaagaagg ttcacttgta
    19981 attgcaaagg catagatagt ctcaatagct gttttcaaag aatcaaactc ccatgcaata
    20041 attggtttat ctaattggct tatagaaaat cctatctttt gaggattaaa ccctccagac
    20101 ataatagtaa cactttcagt taatagatta gatgtatttt tatatacaca aatttgatta
    20161 aaatgcttac acatatcttt agcaaaactt acaatccaat ctagttgttc agtgtagaac
    20221 tttgaaaata ctaaatctaa aatcaatggt ctctttccaa aaaactttgg agattcttct
    20281 ctctttgata catctatacc ccttctatac tttttaaaat acacatcttc atcacttgca
    20341 aatggtataa acatgtttac atattcatct gtactcaata tattctcttt tgtaatataa
    20401 ttcttttcaa tcatcagtac tctttcttct ccaactatat ctctattata tacacttgaa
    20461 tatattaaac ccaataatcc atactttttt acaaattcaa gtacttcttt ctttagtttc
    20521 atttcttttg tctcttttga tatatcttgt aatgcctctt gtcctatgcg aattatatct
    20581 actattatat catctgctac atcaaatggg ttatacatgg taaacacagc atcttctgtt
    20641 ggaactatat ataattcatc ttcttcagta attttatatt cataatcact atacctaatc
    20701 caatcacttg caaaattccc tattaattct atttttttat tcataataat cattcacacc
    20761 tttcaatgta atatataatt tcatattact attacacttt tagtttattc catattttct
    20821 acactgtcaa ctattctttt tatattttta gcatattaaa atttataata gcctttataa
    20881 tggatttcta gagctaaatt tcttgtttat tgatgactaa ttcataat
//
LOCUS       DACULQ010000056        20677 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.056, whole genome shotgun sequence.
ACCESSION   DACULQ010000056 DACULQ010000000
VERSION     DACULQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.056"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            46..1572
                     /locus_tag="JA154_08335"
     CDS             46..1572
                     /locus_tag="JA154_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HAT7995874.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1594..2157
                     /locus_tag="JA154_08340"
     CDS             1594..2157
                     /locus_tag="JA154_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995875.1"
                     /translation="MYMNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGT
                     FILSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCI
                     VAFSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGL
                     EDTMIDMVDALIGTIITIPFIVKKSNI"
     gene            2679..3722
                     /locus_tag="JA154_08345"
     CDS             2679..3722
                     /locus_tag="JA154_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995876.1"
                     /translation="MLKKWFGIVKKRQKSESAKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLECIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            3833..4975
                     /locus_tag="JA154_08350"
     CDS             3833..4975
                     /locus_tag="JA154_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAT7995877.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            5429..6820
                     /locus_tag="JA154_08355"
     CDS             5429..6820
                     /locus_tag="JA154_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7995878.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            7073..8866
                     /locus_tag="JA154_08360"
     CDS             7073..8866
                     /locus_tag="JA154_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAT7995879.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(8947..10329)
                     /locus_tag="JA154_08365"
     CDS             complement(8947..10329)
                     /locus_tag="JA154_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7995880.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            10587..10934
                     /locus_tag="JA154_08370"
     CDS             10587..10934
                     /locus_tag="JA154_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HAT7995881.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            10952..11986
                     /locus_tag="JA154_08375"
     CDS             10952..11986
                     /locus_tag="JA154_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995882.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(12035..13084)
                     /locus_tag="JA154_08380"
     CDS             complement(12035..13084)
                     /locus_tag="JA154_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT7995883.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            13359..14894
                     /locus_tag="JA154_08385"
     CDS             13359..14894
                     /locus_tag="JA154_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995884.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(15079..15765)
                     /locus_tag="JA154_08390"
     CDS             complement(15079..15765)
                     /locus_tag="JA154_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAT7995885.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            15890..17272
                     /locus_tag="JA154_08395"
     CDS             15890..17272
                     /locus_tag="JA154_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAT7995886.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            17674..18942
                     /locus_tag="JA154_08400"
     CDS             17674..18942
                     /locus_tag="JA154_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HAT7995887.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(19035..19391)
                     /locus_tag="JA154_08405"
     CDS             complement(19035..19391)
                     /locus_tag="JA154_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995888.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(19542..20636)
                     /locus_tag="JA154_08410"
     CDS             complement(19542..20636)
                     /locus_tag="JA154_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAT7995889.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         8199 a         2342 c         3423 g         6713 t
ORIGIN      
        1 tgatgggaaa actattacta tttaatatgt aaaggagtat tttaaatgaa tgaatcatat
       61 tggtttttaa actcaagtcc aaaagaatat aacaaattag gtgaaaatat aaaaacagat
      121 tgtcttatag taggtggtgg aataactgga ttgacaactg catatttatt agcaaaagaa
      181 ggtaaaaaag tagtgcttgt tgaggctgat aaaataggat atggaacttc tggaaggaat
      241 actggtaagg taacttgtca gcatgatata ttttactcta agatagagaa aaaatatgga
      301 ttagataaag caaaatcata ttataatgca aataatgaag ctttaaattt agttgaacaa
      361 ataatagaag aaaacaatat aaaatgtgat tttaaaaggg aaacttcttt tgtatttaca
      421 gaaaaagagg atactataaa ggatattaaa gatgaatatc gtacatgtaa aaaaatagga
      481 ataaattgtg agtatcatga aactatagaa aatattccat tagatataaa gggggctatt
      541 agttttacta atcaaggtca atttaaccca aagaagtata ttgatggatt ggcaaaagca
      601 gcagtaaatt tagggctaaa aatatatgaa aatacaccag tagtagacct tgaaaaagga
      661 aaaatatgta gggttaaaac aagagaggat aatataatag aagctgagaa tgttataatt
      721 tcatctcata gtccatggta tgatggattg aatttttatt ttgcaaaaga atatgcagag
      781 cgtgcttatt taatggttgc agttttggaa aataaattag cagatggtat gtttataagt
      841 atagatgacc catcaataac ttttagacaa tataatgatg gaagtgaaaa tttactgatt
      901 tttggaggag gagaccataa agttggtcag ggaggaactg agaaagaaat atttgatgat
      961 ttagagcatt atggtaaaga agtctttaag gtaaaagatt ttaaggggaa atggtctgct
     1021 caagacaata tgagttttga caatgttcct tatattggtt atatcaacaa aagagaagac
     1081 aatatttatg tagctacagg gttctctaaa tggggaatta ctaatggaac tgctgctgga
     1141 ataataataa aagatttgat aataaataat aattctgatt ataaagatac ttttaatcca
     1201 tccagattag gtagctactt ttcaaaagat tttataaagg aaaatgcaaa cgtagcaata
     1261 aattatgtaa gtggaaaatt aaaaataggt agtggagata tgcctaaaaa taatggagaa
     1321 ggaaagattg ttaatattga tggaaaaaga tatggtgtat ataaggatga taatggagat
     1381 ttttatattg tagacactac atgtactcat ttaggatgtg aacttaattt caacagtgaa
     1441 gaaaaaacat gggactgtcc atgtcatggt tctagatttg actataaggg aaatatactt
     1501 gaaggaccag ctcttaagcc attaaaactt tatggacatg gtgataatga tgttaatcct
     1561 aagttattat aaaattattt ttaggaaggt aaagtgtata tgaataaact tttaaagcca
     1621 ataactatac tattgtatat agtttatatt ttatctgccg tattttttgt agcaaacaaa
     1681 gaatatggaa gtctaggtct ggtagcaatt agtttagttg gtactttcat tttgagtttt
     1741 ataaataagc aaacggaaag attattgagt aaagaattat atataatgct tgctttattt
     1801 ataatgtttt catcattgtt aggtacttgt tttaattttt atgatataaa ttattatgat
     1861 gattttttgc atttatggtc aggtataata tcttgtattg tggctttttc tataattaaa
     1921 tacttttata cacaaagaga gataagtaaa atgagtaaag tgtttttagt tatattttta
     1981 tttatgttta gtatgggagt agcgagttta tgggaaatag gtgaattttt aatggatact
     2041 tttgttggga ctactacaca ggccggaggg ttagaagata ctatgattga tatggtagat
     2101 gctttaatag gcacaattat aactattcct tttatagtaa aaaaatctaa tatatagttt
     2161 taaatttttt atattaatta taatggattt aattaatcat gttgagtttc aaggtctata
     2221 tgattaatta aatccatttt tgttatttat aattattatt gaatatattt ttacatataa
     2281 gatataaggt gatagataga aattgttgga ttatgtaaat aattaatata taataccatt
     2341 atgggcaata aatacaaatc aaatgataat atagtctatt tatgtaaatg ttatattgta
     2401 tactaagtat aatagtaagt tttttgatag tggtgtagaa agtaaaaatc tactagccta
     2461 aattgatgta tggattagtt taggaacagt aattgcatat cttaagtttg atgtcatgtt
     2521 gggctactag aaataggtca tctttttcac ttgaatatct tttctttaaa catggagagt
     2581 atcatatcaa aaacatatga aaattaagtg atttataaga acagtaatat tttatatgat
     2641 agtatagaat agtaataaag tattaggagg aacattgagt gttaaaaaag tggtttggta
     2701 ttgtgaaaaa aagacaaaaa agtgagtcag caaaagaaga aggtgaagta atattaaaaa
     2761 acgaaaaaat attatctgaa gaaaagttga tagatgaaga aggagttgta gttaatattg
     2821 ataatgaaat attaactaaa gtagaagtag taaatgatga taatgaaata gaagaaaaaa
     2881 taatagaaga agattggtta attagtgaaa ataccataaa attagatgat aaagaagcaa
     2941 ttattaatga taaaaacata gaattatgta aaaaagaagt tcaagttgaa agtgaaaaaa
     3001 taggtttaaa taagtttgaa ggacttgacc aaaatgaaaa ttataatcta gaaaaaaatg
     3061 ttattgaaga aaaaaaagta agtgaatgtt tgacagaaga agatttagag tgcataaaag
     3121 aaattaaaat aaaaagagga aaaagtataa aagctataaa cttgtatact aaagaagagt
     3181 gggtttttga cactcatata cagtgtagta aaaaactcaa agttccatta gggtacataa
     3241 gagaaaattt aaaatatgga tatatggatt actttggaga tgcaataaat tatttaagtg
     3301 aagtattaaa tatagatgaa tactgcaaaa gtgaatggag ctatctcgat aatagcaaat
     3361 ctccatctga gatatttaat attctaaaca ataaaatatt tagtataagg ctttctaatg
     3421 aaaaaagaaa tgaaattttg acaaatgata aaattgaagc attaaaaatg aattatagat
     3481 ttgaatgtat tgatgaagaa tatgatgaat attttaaaaa atataagtct ataatcaaaa
     3541 gaggtggaaa gaaaaaagtt gaattagtaa ataaaaaagg tgacatttta gaaatattta
     3601 agtctttaga agaatgtgcc atttatttgc aaaaggaaaa gaatgaagtt atacaaatgt
     3661 taaaatatgg agatacaaaa gtaggaagaa actttataag gtatagtttg agaagtattt
     3721 aatttcattt taaattcata agagtttcat ttctgattca cacatttaat attattttaa
     3781 tgtaaaataa taaaggtgaa gtattgcaca attaaaatta aggagggtat taatgtctga
     3841 agaaaaaaat ccaaaagaaa atacattaga tgaatctaaa aattcagata atgtaaattc
     3901 taaaaaagat gatgtgtcag atgaaaaaaa tactgataac aaagttaaaa aaagggaaat
     3961 aaagctaaac aacaaaacac ttaaagctat tgtagcaatt gttgcagtag gagctatatg
     4021 tttaggtgga ggctatgttt ttggcaaaga agctggaaga aaattgcctg ctacttcaaa
     4081 acactataat agcagtgagg tagtagctac tattggggat aaaaaaataa caggtgaaat
     4141 gttaagaaag agaatggagc cattatttta tttaaatgca aaaactacta tgactgatga
     4201 cgaaatacgt tcatatgaat caaatatgat tgaaactatg attacatctg atatattatc
     4261 ctctgaagca gcaaaagaga aaataagtat aactaatgac caactagatg ctcagtataa
     4321 taatttaatg agtagtatag aatcagcaat gggaatgact aaagaagagt ttttaaaaca
     4381 gtttgatttg actgaaaaat atataaaaga tgatatgaaa aaagagctag tagcatcaaa
     4441 atatttaaat gaaaaatcaa aagttactga taaagaagca gagaactatt acaataaaaa
     4501 caagagcaat tatttacaag ttagagcttc acatatttta ataaaaactg ttgatgataa
     4561 aggtaagcaa gttagtagta gcaaaaaagc tgaacttaag aaagaagctg aagaaatact
     4621 taaaaaagcc caagctgggg aagattttgc tactctagct aaaaaatact ctgaagatag
     4681 tagtgcagaa tcaggtggag atttaggttt ctttggtaaa ggtcagatgg tggaatcttt
     4741 tgaaaaagca gcctttgcat tgaagaaggg agaggtatct aacaaacttg tagaaagtga
     4801 ttatggatat catataatta agaaaactga tgaaaaatat cagccatttg aagaggtaaa
     4861 atcagattta gtaagcagtt taacatcaga aaaacaaaac ctacttatac aaaatctaaa
     4921 agaaaaatat aatgttaaaa ttacagataa atatcaaatg aaagctaaag aatagataaa
     4981 gtcaatttat taaaaaaaag aaaaattaac tgtatggata aaagctattt ttactcttaa
     5041 tatagagagt ataaaatagc ttttattatt ttgcaataaa atctatttat atatatgaag
     5101 tgacaaagaa ataacatgca aaaagatatt aaaaagaaat atttttaatt tgaatttttt
     5161 caataaaaat ttacaaaaat gaaaaaacaa acaaaaaaga attgaagatt taagttgtta
     5221 tttaaatata tatatattaa gattttctgc tgaagatact aaaaaaataa tatttttcat
     5281 aattttaaaa ataataataa aaaaatgaaa atacaggcga taatctattg agtaaacata
     5341 gaaaagatga tattataagt atgtgctaaa atggttaaag caatattaat attaaattta
     5401 actataaaac aaaagggggc gtttttttat gtcaagtaca gaaagcgtat ctaaaaaaag
     5461 tggtcatccg aagagttttt ggcttatttg catgacaatt ttgtgggaaa gatttagtta
     5521 ttatggttta acagctttat tggtattatt ttttacagca agtgtagctc aaggtggttt
     5581 aggtctttct actcaagaag caacatccat gtttggctta tttacaggtc ttatatatct
     5641 tacaccatta gtcggtggct ggttagcaga taaagtttta ggtcaacaaa aatgtatttc
     5701 tataggtgca cttttaatag caataggaga tttcgtacta tttgcttcat ctacatctat
     5761 tgcaatggtt tgggtaggtc tatcaataat tattttagga aatggttttt ttaaatcaag
     5821 ttgctcaaat atagttgggg atatatatcc taaaactgat gtgtcaagaa aagatgcagc
     5881 atacagttta ttctatatgg cagtaaatgt aggagcattt atagctccaa taatatgtgg
     5941 attgctttct gataatgtat ttgcagtaag aggagcaagt ggagatatag tatcttatgg
     6001 atataagatg gcatttttaa tttgtggtat aggcatgatg ttaggtatga ttatatttac
     6061 acttcttgca ccaaaattat taaatgaagt cggaaaatat cctgtgacta aaggtaataa
     6121 aggtgaaaaa attgagtatg gaccacttac taagttggag aaaaatagga taatagcaat
     6181 ggttatttta tttttatttg tggtcttatt ctggacagca tactatcaaa cacaaagttc
     6241 ttttatgatt tatgtaaggg ataatgtaaa tagaactcta tttggatttg aaatgccagt
     6301 agcatggctt acatcactaa atgctatatt atgtgtggct ttatgcccag tattagctac
     6361 cctatgggtt aaattatctc ataccaaaag aggtgatttc tctattccag ttaaaatggg
     6421 attaggaatg ataataatgg gtgttgggtt tatagttatg gtatttgcta cattagctaa
     6481 tggtccagat ggaatggtaa aggctagtat attgtttata gtattgactt atatatttac
     6541 aacagtaggt gaattattct tatctcctat aggacttgca atgttcaata aattagctcc
     6601 agctaagtat gctacgttgt ctatgggagc ctggtatcta acaagttttt ttgcaagttt
     6661 attatcagga aagattgctg gttttacagc cacacttggt tttttacaga tatttggagg
     6721 aatagcaaca gttttaataa tatttggttt aatattattg ggaataagaa atgtattaac
     6781 aagattaatg gctatggatt tactaacaaa agaaaaataa ttcatgacga attattgtaa
     6841 ataaatgaat tactttaaat aaaggaagtt acatttaaat ataaaattaa atgtaatttc
     6901 ctctttgatt atgaaaaaaa gctttacaca aatagatatt ataattaaaa ttataattgt
     6961 gataattaga gtgtataaga tttatataaa ttatacaaaa aatatttatg taataaactt
     7021 tacatactta tatatttagt tattcaaact attatttcga ggaggtcttg ttgtgaatat
     7081 aaaagataga ttaagtggat taagaaaatt tatggaagaa aaaaatatag atgcctatat
     7141 gattccatct tctgataatc accaaagtga atatgtagga gattacttca aatcaagaga
     7201 atttatatca gggtttaatg gttcagcagg gacggttatt gtgactaaag acgaagctgg
     7261 gttatggaca gatggaagat attttataca ggcagaatct caattagaag gaagtacaat
     7321 aaaattattc aaaatgggac aagaaggttg cccaactaca gatgaatatt tatataaaaa
     7381 tattcctgaa ggaggaactt taggatttga tggtagagtt atatctgcta gagaaggagc
     7441 tacattagct gaaaaattat ctaaaaaagg tataaagata gaatatcagt atgatttaat
     7501 agatagtata tggccagata gacctgcttt atcagatagt aaagcatttt tattagatgt
     7561 taaatattgt ggagaaagtt ttagctcaaa attagctaga ttaagagaaa aaatgagtga
     7621 aaaaggtact tctactcatg taataactac tcttgatgat atagcatggt tattcaatat
     7681 aagaggtgga gatgttaaat ataatccagt tgtactttct tatgcagtaa taactttaaa
     7741 agaagtttat ctatttgttg atgaaagtaa gttaaatgaa gaaatactaa atgaactagc
     7801 taaggaaaat gttcaaataa agccatataa tgatgtgtat gaatttgtaa aaaatataga
     7861 caaaactgaa aaagttttat tagatggaac taagcttagt tatacaattt acaataatat
     7921 accttgtgaa gttgagaaag ttgatgagtt caatccagta atgtttttta aagctcaaaa
     7981 aaatgaggta gaattagaaa atataagaaa ttcacatgta aaagatggtg tagctttcac
     8041 taaatttatg tactggttaa agaaaaatgt aggtaagatg gaaatcactg aaataagtgc
     8101 tactcaaaaa ttagaggact taagaagaga gcaagaaggt ttctttgagc caagtttcaa
     8161 tacaattgct gcatataaag agcatgctgc aatgatgcac tatagtgcaa ctccagagtc
     8221 aaattataaa ttagaggcag aaggattatt tttagtagat tcaggtggac aatattatga
     8281 tggaactact gatataacta ggactacagt tttaggacct ataagtgatg aattaaaact
     8341 acactttact tctgtagcaa gaggaatgat aaatctttct aaggctaagt tcttgcatgg
     8401 ttgtagagga tataacttag acatactatc tagaagttgc atgtggaata tgggaattga
     8461 ttatcaatgt ggaactggac atggtatagg atttgtgcta aatgttcacg aagctccaaa
     8521 tggatttaga tggagagtag tacctgaaag atttgatagt gcagttcttg aagaaggaat
     8581 ggtaactact aatgaaccag gtatatatat agaaggttct catggtataa gaacagagaa
     8641 tgaaattgta gttagaaaag ctgaaaagaa tttctatggt caatttatgg aatttgaagt
     8701 tgttacgtta gctcctattg acttagatgg tatagtacca gaattaatga ataaggatga
     8761 aaaagattat ttaaactggt atcataagtt agtttatgat aaaatatcac ctttcttaac
     8821 tgatgaagaa agagaatggt taaaagttta cactagagct atatagtttg tactagatac
     8881 aataaaaatc gaggtattat aatgcctcga ttttttattg tatattttat taactttaaa
     8941 aaatttttaa tttgataatt ctactaattt tttattcatt ataagtagta taattccaaa
     9001 tatcaataaa aatgatggta tcactatgaa gacttgtaac ataccaagtt ggtctataat
     9061 agcttgcgta tagccagata atttatttgc tgcaaatttg cctaaaaacc aaacacccat
     9121 taggaatgaa atatactttt caggcgaaag tttatttacc ataccatacc cgactggaga
     9181 aaagcaaatt tctcctatac tttggaatgt taaaaatcca actacccaaa atacacttgc
     9241 tttgtgaatt gaattagggt ctgttcctgt ttgtataact gctactatca taaacaaaaa
     9301 tgctatggct aatagtataa aacctaagct catttttttt ggcacactta aatcgccttt
     9361 tttagtttga gataatttta cccatactaa agcagagata ggtccaagaa taacacaaag
     9421 gagaccattg tatgaatcta tccatgaagt aggaatttga aaattgccaa cagtaagatt
     9481 tatgtgttcc tttgtataga gtgcaataga catagaggct tggttatatg ctatccaaaa
     9541 aaatactgtg aaaaaagcta acaccaatat tactattatt ctttttttct caatcaaagt
     9601 taaaggtctt gaagctagca ttttcttata ttctagtgat tttcttatag actctctccc
     9661 gttactaggg tcattattca aaggtttata aaactttctg tttccaaata taaagaagaa
     9721 taaacctatt agaaccgtaa ttgatgaaaa taaaaataca tatctatatc catacatact
     9781 tatagataat ccgcttttca tagcaaacca ttcattagca actaaaccgc atattaaagg
     9841 gccaaataag gtacctagat tagtagccat atacgtaata gagaaagctc catcttttct
     9901 tgggtcatca ttggtatata agtttccaac catagtatgt aagtttcctt tataaaaacc
     9961 tattcctaat ccagataaaa atatcatgat gtttatatcc atcttactat ttgctatgta
    10021 tcctagaaaa taaccaattg aaaccattag cattccaagt ataatacagt ttttgggacc
    10081 taaaaatcta tcactgaccc atccaccaat aactggagag aaataagtcc atgctaaaaa
    10141 agatgcaagc attgatgcgg attctgctgg tgttaaccct aaaccacctt tggagacttg
    10201 ttcagctaaa aacaataata ataccaattt cataccatag tatgcaagtc ctacaaaggt
    10261 aaatattgaa cagcagaaat ataaatctct aggttgttta agtttttctt gcgtttttaa
    10321 aactgacata tgtcgagaac ctccagataa atagtttaac aaaattttac catactatat
    10381 gagtatatgt ttttttcata tagacgtcaa cttgtaagat aatttaatta agtcgaaata
    10441 tatgtaaaaa tagtatcatt tttgtgtatt cagtaatatt tttttatgat aatatatgtt
    10501 attattatat taaaatacaa acatatttat ggaaaggata tgccaattta gtagttagat
    10561 tgattgtttg gcaggtggga tgagctatga taagaaagaa atctgaatta caagtagaag
    10621 taaaagaaaa tttaaatggt ggtataggaa aggttaagct tgaaaacata atacaaaatg
    10681 aagagcttaa aggaaaagga agactattta aaagagtagc tttaccagta ggaagttcaa
    10741 ttggtgtgca tgaccataca acagattttg aagtatatta tatactaaag ggaaaaggta
    10801 aagtatttga taatggggaa tttgtagaag taaatgaggg tgatgtagtg tatacagctg
    10861 atggcgaaaa acattctata gaaaatatag gagaagaaga tcttgagttt gtagcactag
    10921 ttttatatat gtaaaatatg gaggaggagt catgacaggc aagatagaag aaattaagga
    10981 caagataaat agattttgca actccaattt aaatcaagaa tacaaagata taagctttaa
    11041 aattttgcaa aaccttttag ataataataa agagataatt cattcttcta gagctgatat
    11101 atggtcttca gctatactaa atatagtttt agaacaaaat cttttataca ataagaagca
    11161 tccactacat ataacaaaaa aagagttttc aaaacaaata ggtgtttctt taaatactat
    11221 aaataataaa tcgtctttaa taaaagatgt ttgtagtatt gactttttaa atcaagatac
    11281 tatagctatt tctaatgtag aattttgggt aaaagagacc aattccaaat caaagtctgt
    11341 agatttagat ttagaaaaga aaaaaatact atataaaaga tatattaagc tatcacaaga
    11401 tgcgaatagt gatattgaat caattagata tatggaggaa gctgtaaata taggtaaaag
    11461 tatgattact aaagaagata aagaacttgg attttggaaa ggtttatcta ccagagccta
    11521 tatgatagca cttgaatccc ttgctagaaa gttagaaagt attaataatc taaaggagtc
    11581 caaaaaagta ttagaatatt taatagaaat taatcctagt gatgagcagg gaataagata
    11641 caaattatta aatgtattaa ttagattaaa tgatagaact tcaataaata aattatttga
    11701 actttataaa gaagaaaaat ctgctacatg gatgtacagt aaagctcttt attactttaa
    11761 aaataaaagt atgtttttgg caaatgattc tattaaggtt gctagaagta aaaataagta
    11821 cgttggatta tatttgttag attggcgaaa tgcttttggt agagaatttg ttacagagga
    11881 agaaaaggca gaagctgtct attactatga tgaaaacata gtgatttgga atgaagttaa
    11941 aggttctatg gattggctat taaaaaaaat gttggaattt tcataaaaaa aactactcct
    12001 tacttttata taagtaaaag gagtagtttt taatttatac atctagtttt aaatcttcag
    12061 gttttattag accttttttt ctcataaatt ctgaaatcaa caaagttaca atagcaggaa
    12121 gtataaaatt aagtaaaagt ataccaaccc acatagatat acctcctttt ccagcacttt
    12181 gcatagcagt tactgttcca aattgtccaa ccaatccaca agtacccata ccagaaccaa
    12241 taggaatatt ctccattttg aatattgttg tagataatgg tccaagtaat attgaagcta
    12301 cagtaggtgg tatccaaatt ttccaatttt ttattatatt aggtacttgc aacatagatg
    12361 tcccaagacc ttgtgccaca aggccgccaa ctccattttc tctaaagctc ataacagcaa
    12421 agcctaccat ttggcaacaa caacctacag ttgctgcacc accagcaaga ccacctaaac
    12481 ttaacatcat acaaatagct gcactactta taggtaatgt aagagctatt ccaacaagtg
    12541 cagagactaa agctcccatt attataggtt gcatagttgt agcttgcatt attatatttc
    12601 caaatgaagt cataaaagca gaaacagctg gaccaataag ttcagctaca agtactccaa
    12661 ttagtattgt tacagcaggt gtaaccaata tatctatttt tgtttctttt gaaactaatt
    12721 ttccaacctc tacagcaaaa agtgtagcga caaatgctcc aacaggtcct cctaattcat
    12781 taccacaagc tccaacaagt gcacaagaaa atagtacaag tggaggtgct tgaagtccat
    12841 acgcaatagc cactgctata gctggaccag taacctgttt agcaagaggg cttataactt
    12901 cagtaaaaag agaaatattg aaattatctc ctaatgtatt gaaaattgtt ccaattaaaa
    12961 gggacgaaaa aagaccgagt gccatataac ttagtgcgtc aataagatac ctttgaacag
    13021 aaatttcaat attttttctt ttagcaaatg atttgaaatt tcttttttta taattgattt
    13081 ccaatttaaa atcctcctca tataatataa gtataatttc tttttaatta attatataac
    13141 ttttcagctg acaagacaaa tgttatttca ctaaaattag actattgtaa tagtaagaaa
    13201 ttaacattaa ttttagtttt aaattttaaa tttatggttt ataattagta tagggatatt
    13261 ctgaaatata ataattttcc agtttatccc aatatttaac ttaggtagat tgcttacaaa
    13321 acaaatgatt atatattagg ctaaaaggaa gtggatagat gatatttgta aaaaataaga
    13381 aatacagaaa aattgccata attatatcta ttcctatatt gcttgttgcg cagtactacc
    13441 tttttaaatt tggaatactt agaccaatgg taaagggagt agaaattgaa attgtagatg
    13501 gggaatatgt taaagatata gataaatatg tagtaaaatt aaatgaaaca gttactttgt
    13561 ctacaggtga atatattaag gttccttcgt atgctaaaaa tccaaatatt tggtttaatg
    13621 tattggataa ctcagggact ttaaaaatag atggaaataa aatgacagca ttaaaggttg
    13681 ggtattcttc tgtgggtatc atgaaaaatt caagagtatt aaaaaaggct actataaaag
    13741 tagtagaccc agaggtagaa tcactagata tggagattaa tggggattta aaacatgttg
    13801 gtgatagtgc tactatagaa agtacaatag aagttgatta tgataagttt aaaaaatcat
    13861 ataagccagt ttataagagt agcaatgaaa atattttaaa aatcaaagga aataaagtaa
    13921 gtgcagttgg agttggtaaa gctacaatat atgctaagtg tggaaataaa gaaattgaga
    13981 gaacatttag gattcaagct agagttgctg aaataaatat ggatgatata aaaatagaag
    14041 ttgaccagaa tgaaaaattg aaaccagata taataacttc tccacctggt ttagaaccac
    14101 caaaaatata ttatgagtta gtagattcaa aattacctgt agaaagagca ataagtttaa
    14161 gtagtaatgg aaatgttgta gggcttagag aaggtaaaga tagggtgaga ataacttgtg
    14221 gagataaatc taaagtaata acagtaacag tagtcaaaga aactataacg aatgactata
    14281 tacaaaactt ggttggttca cataaaatag taaacaataa agttattatt aaacttgaat
    14341 gggattatat gaaagatgtc tttgattatg atatatatct taaagataag ttgtcaggag
    14401 aatcatttaa gaaaattaaa tctataacta taaatgaaag tgatttaggt aagtctaaca
    14461 aggtatatgc aactatagaa gtagatatga agggtaatca aaatatagat tttgatattt
    14521 atgtcatagg taaaaaggat tcagatacaa ctaagccaag taatgtaata aatattacca
    14581 atggaaatgg aaattcaaat aatgataata atattaaaga tatgagggta gaaaatatat
    14641 cagctaatgt tgatagagat aataatgtca ttagagttaa ctggagtagt atatctcata
    14701 aggactgtac ttacagtata tatgtaaaaa ataatactaa tggagatgga gaatttactc
    14761 tatatcaaag tgatatacag ggaaatgagc atactataag tattcctgaa ggagatttaa
    14821 atctagatat ttatgttgtt gcaaattata atggaaatag ctctcaaaat agtgatgtta
    14881 taaatgtaag atagatttgt atacaaattg aaagtgttga gtttatttaa atattaaatt
    14941 aagaaaaagg atagtatagg gtaattatac tatccttttt tgttacaata tataggttta
    15001 aaatctctag taatataaat tataaaaagt cttatttaca caaatctata gatatttttt
    15061 ttattaatca atattgtctt aaattgaact tagtgcggct tctattgcat gtattactgt
    15121 cgtatcaaat agtggaatag aagtgtcatt ttgtttaatt aaaagaccta tttctgtaca
    15181 tcctaatata acagcctctg caccttgttt gccaagctta tctataatag ataagaaagc
    15241 tttttttgaa gattcagata taattcctaa gcaaagttca ttaaatattg tgtcatttac
    15301 tattatacgg tcttcttcat ttggaattaa gacttcaatt ccattattaa taattacatt
    15361 tttataaaaa tcttgttcca ttgtatattt agtacctaat aaggccactt tttttatttc
    15421 tttttccctt aataccgtag cagttacatc tgctatgtga agtaatggaa tatgtatact
    15481 ttcttgtatt ttatctgata ccttatgcat agtattagta cagataacaa taaaatctgc
    15541 ccctgcctct tgtaatttaa ttgcagcatc agctaaaatt ttggcgcttt tttcccaatt
    15601 tccactagat tggcattctt caatttcttg aaaatctaca ctgtaaagta tacatttaga
    15661 agagtgtagt ccacctaatc tttcttttat tactgtatta ataacttgat aatatgtaat
    15721 ggtactttcc caactcatac caccaattaa gcctatagtc ttcatattat acctctctta
    15781 tttatttatt ttgaaatagc tatttacaaa tacaagtata gcatctaaaa tagaagcata
    15841 caataataaa attgtatgga gtacaattat aattaagagg agtaattata tgccaataaa
    15901 ttcttttgaa aactatccta tgacatggaa accaaagtta gataaccgaa aaccaccaat
    15961 atataaaact ttagcaatgt tattagaaga agatattaaa agaggaaatt taaatcctgg
    16021 agataaattg cctccacaac gggaattagc agattttcta gatttaaact taagtacaat
    16081 aacacgagca tttaaacttt gcgaagaaaa gggactaata tgtgcaaaag tgggaaaggg
    16141 aacattcatt tcttctgatg taaatgtaag taatacatta ttatatcaaa cggaatcaaa
    16201 agacataatt gagttgggga cagttcatcc tccgtatgag caaaatacat atattattga
    16261 ctttattaaa aatgtactaa aacaaccaga aatggatagg tttttacaat acatgtctcc
    16321 atctggcaca tatatgcaaa aaaagtcaat tgcaaaatgg atagaaagaa ataatgtata
    16381 caccagtgaa gaaaatatat tattatctac aggagggcaa aatgctatat gtgcaacttt
    16441 actaggatta tttaaagctg gtgatagaat tgctacagat tctttaagtt tttctggaat
    16501 taagtcaata gctaaaatga taggtattca attagtccca attcctcaag aaaataatga
    16561 aatatcgatt gaatatcttg aaagttattg taaaaatgaa aatataaaag gcatatatct
    16621 tataccagat tatcataatc caacgacaca tactatgtct gatttatctc gtaaaaagat
    16681 agccaagata gcaaagcaat ataatataat aattattgaa gatgctataa atagcatttt
    16741 tagagatggt atacaaactc ccatattttc tttagctagt gataatacta tatatatttt
    16801 tagtacatct aaatttttat gtgctggtct tagagtagca tttgtagttg ctccaaaacg
    16861 atatatagaa aatttagaaa atgcactata taatatgaat ctaatggttt ctccatttac
    16921 agcagaaata gtacaccgtt tattatattc tcctattata gataaaatta tagaagagaa
    16981 aagagatgca attatagaaa gaaatgaagt tgctgataaa gttttgtcag attataattt
    17041 gattggtgat aagaattgca gttttcgttg gttacttctt cctgatgaat tggatggaaa
    17101 atcatttgaa atttctgcaa aaaatctagg agttcaagta tattgtgccg aacgattttc
    17161 agtaggaaac tctacagttc caaaagcagt tcgtatttgt gtaacagctc caaaagatgt
    17221 agaagaatta gaaaaagggc ttaatataat aaagtcactt cttaagagtt aaatttattt
    17281 tactattatt taattaaata aataaaaagg cggagaaatt tattataata aacctccgct
    17341 tttattattt aatgaaatac tttacttttt attaaaaaat ttaattttaa taatcttgaa
    17401 aatataatcg ttttatggta aaataattct taaaaataga aaaaattaat catttgttaa
    17461 aaaaaatgca taatttattg taatttattg acgaaggttg aaaaatattc ataaatgtgc
    17521 gaaataacag aaaaaatgca aaaattaatt caatttcatt tgttataaaa tctatatggc
    17581 aaaattaaat ctgcctatca gaatgatata aacacatcag aaaggaggac gctatcaaat
    17641 aatacaagta gttaactttg atagcaaaaa attatgaatg aacaaactag aatatcctta
    17701 gagagagctg ctgaattaaa atcaaaaatt gatgattata ttcaggctag aaaaacgatt
    17761 aacagaggtc ttgaaaaaga agaagagata aataaacgaa aacagaaaat attaagtatc
    17821 ttaaatggaa ctgaagagga ttggaataac tacaaatggc aattatcaaa tagaataaca
    17881 gatgtagata ctttatcaaa aattataact ctaactaaaa aagaaaaaga atatataaaa
    17941 gaggttggta ctcaatttag atgggcaata tctccatatt atttgagtct tatagaccca
    18001 gaagatatat gtgacccaat aaaattactg tctataccaa cacatataga gttggaagat
    18061 gagcaagaag atttggaccc aatgggagaa gagtatacaa acccagcagg atgtataact
    18121 agaagatacc cggatagatt aataataaat gtaacaaatg agtgtgctat gtattgtaga
    18181 cactgtcaga gaagaagaaa tattggacaa caagattctc ataagtcaaa agctattatc
    18241 caagaatcta tagactatat cagagaaaat gaagaaataa gagatgtact agtaactggt
    18301 ggagatgctc ttacattaaa agatgattat ttagagtgga ttcttagcca acttaaagag
    18361 ataccacatg ttgattatgt tagattaggt actaggactc ttgttacaat gccacaaaga
    18421 attacagatg aattttgcaa tatgctaaaa aaatatcacc ctgtatatat aaatactcat
    18481 tttaatcatc caatggaaat aactaaggaa tctaaagaag cttgtgaaaa gttagcaaat
    18541 gcaggcgttc cattaggaaa tcaggcagta ttattaaatg gaataaataa tgataaattt
    18601 gtaatgagat gtttaaatca agaattactg aaaataagag taaaacctta ttatatattc
    18661 caaagtaaac atgtaaaggg aacaaaacat ttcaatacat cagtagatga tggtcttgaa
    18721 atcatggagt atttaagagg atatacatca ggaatggcta taccaacata tatagtaaat
    18781 gctccaaaag gaggaggaaa gactcctttg cttcctcaat accttgtatc aaaaggaaca
    18841 gattacgtta tgcttagaac atgggaagga aaagttataa aaatggaaga tgaacctgct
    18901 gtagatataa agaaacttat aaaagaacaa gcacaggatt aaaaaaatat ttaaagttta
    18961 taaaaagaaa caactttaaa ataaaaagaa aatggatgtc aaatttatat caatttgcat
    19021 ccatttttta ttagttattt attattttgt tgttgattaa ctaattcaag atatccactt
    19081 gggtggaagt aatatatctc agaagtattt tcatttacaa agtaaccaat atcaccatct
    19141 gcatctggta aaaatacata accattaaga tttttagctt ttaatgcttc aaaatcacta
    19201 gcatttcctt gatatatgta atcaacttca ttgttgtatt ttactaaaag ttctttagct
    19261 tgttcttgag tcaatttagt attttggctt acaggaacat tcttttgaga atctgcataa
    19321 gaagattcat ttactggtgc agttaataac aaacaagata aaactaataa agatataagt
    19381 gcttttttca tactaaaacc ctctttcatg tttaattata taagattcat tacttaataa
    19441 tattgtatgt ttcctataat taaatattac cattaaagag aataatatta cagttacaca
    19501 gctgtaactt ttgattacaa aatcgtaatt aaattgtatt tttattccat ttgtatttta
    19561 tcatgtctac gatttacaaa ctcacagaag tatatcttta taacgcccat cacaggagca
    19621 gatagaatca taccaggtat accaaagaat cctccaccaa cagtaactgc taggattgta
    19681 aagaatgggc taagacctag ttgtccacct acgatttttg gctctataaa tgtaacttca
    19741 acttgttgaa ttattattaa gtaaattaaa gaagttaaag caattgttgg attggagaat
    19801 aaattaatta ttacaactgg tgccatacct attactggac caaaatatgg aaccatattc
    19861 atgacaccca taagtgtacc aaataatagt gcatattcag attttaaaaa gtaaagacca
    19921 attccagaaa gtactccaac tataaaagaa tctaaaatct tacctgtaaa gtatttacca
    19981 atatttaaat ttaagctttg acatagttct attataaagt ctccatattt tttacctaag
    20041 gaaatataaa ctactttttt tgtaaacagt aagaattttt ctttttctaa taatatatag
    20101 aaacaaatta taaaacctaa aagaaattgt actataaatt ttccaactga aaatgtagtg
    20161 ctaaatatcc caccaagtga accaataagc aagttactaa atttaggcat tacactcata
    20221 attttgtcac catactcttt taatgtatta gggtcaactg attgtaaact cttgcctaag
    20281 tcgaataaaa actgctctgt tttatctatg tatgtaggaa tttgagcaac taaatcagct
    20341 aaactattta ctatggatgg aactgtaaat aatataaacg aaattagtat aactatgagt
    20401 acaccataag taagaagtaa cgaatatatt ctcttaaaat ttaatttact ttctaagaac
    20461 tttactagtg ggttgaatat atatgctaat acaaatgcta ttataaatgg tgttagtagt
    20521 gacattaata aacttatcac tccaaaaaaa tatttgtaat tatcaataac tttaatcaag
    20581 atatatgaaa caactacagt gagtacaata tttagataaa tttttttatc ttgcaaaata
    20641 acacaacctt tcttatattt attaaattta gatatac
//
LOCUS       DACULQ010000057        20601 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.057, whole genome shotgun sequence.
ACCESSION   DACULQ010000057 DACULQ010000000
VERSION     DACULQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.057"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1098
                     /locus_tag="JA154_08415"
     CDS             <1..1098
                     /locus_tag="JA154_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT7995890.1"
                     /translation="YKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVYKSDKETF
                     TYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKFKSKKINR
                     FSYKTNFTNGNIMYCGQYIKLPKLGMVKIRDKQVPQGRILNATISKEPSGRYYVSLCC
                     TDVDIEVFENTNNHIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTI
                     GSLNRNKARLKVAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLS
                     RLISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIREWI
                     CPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            1216..1521
                     /locus_tag="JA154_08420"
                     /pseudo
     CDS             1216..1521
                     /locus_tag="JA154_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            1534..2439
                     /locus_tag="JA154_08425"
     CDS             1534..2439
                     /locus_tag="JA154_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAT7995891.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            2570..3499
                     /locus_tag="JA154_08430"
     CDS             2570..3499
                     /locus_tag="JA154_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FMN
                     adenylyltransferase"
                     /protein_id="HAT7995892.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            3499..3597
                     /locus_tag="JA154_08435"
     CDS             3499..3597
                     /locus_tag="JA154_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995893.1"
                     /translation="MYDKMIKENNHYSAFEYSNQCLVIGVSKKIRR"
     gene            3603..3860
                     /gene="rpsO"
                     /locus_tag="JA154_08440"
     CDS             3603..3860
                     /gene="rpsO"
                     /locus_tag="JA154_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087812.1"
                     /note="primary rRNA binding protein; helps nucleate
                     assembly of 30S; binds directly to the 16S rRNA and an
                     intersubunit bridge to the 23S rRNA; autoregulates
                     translation through interactions with the mRNA leader
                     sequence; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAT7995894.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            4100..4945
                     /locus_tag="JA154_08445"
     CDS             4100..4945
                     /locus_tag="JA154_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAT7995895.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            5070..7181
                     /locus_tag="JA154_08450"
     CDS             5070..7181
                     /locus_tag="JA154_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAT7995896.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            7447..8190
                     /locus_tag="JA154_08455"
     CDS             7447..8190
                     /locus_tag="JA154_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HAT7995897.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            8348..9595
                     /locus_tag="JA154_08460"
     CDS             8348..9595
                     /locus_tag="JA154_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAT7995898.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGI
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            9630..9875
                     /locus_tag="JA154_08465"
     CDS             9630..9875
                     /locus_tag="JA154_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HAT7995899.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGDILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            9885..11108
                     /locus_tag="JA154_08470"
     CDS             9885..11108
                     /locus_tag="JA154_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAT7995900.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            11221..11955
                     /locus_tag="JA154_08475"
     CDS             11221..11955
                     /locus_tag="JA154_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease"
                     /protein_id="HAT7995901.1"
                     /translation="MKDYREDNEKFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            12078..14489
                     /locus_tag="JA154_08480"
     CDS             12078..14489
                     /locus_tag="JA154_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAT7995902.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            14492..15550
                     /locus_tag="JA154_08485"
     CDS             14492..15550
                     /locus_tag="JA154_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAT7995903.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            15544..16878
                     /locus_tag="JA154_08490"
     CDS             15544..16878
                     /locus_tag="JA154_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAT7995904.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16865..17407
                     /locus_tag="JA154_08495"
     CDS             16865..17407
                     /locus_tag="JA154_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAT7995905.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            17695..18741
                     /locus_tag="JA154_08500"
     CDS             17695..18741
                     /locus_tag="JA154_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HAT7995906.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGISVSKEIVEE"
     gene            18917..20458
                     /locus_tag="JA154_08505"
     CDS             18917..20458
                     /locus_tag="JA154_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087825.2"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAT7995907.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8442 a         2093 c         3966 g         6100 t
ORIGIN      
        1 tataagttta gaatgtatcc aaataaaaaa caacaagagt taattaacaa aacttttgga
       61 tgttgtagat ttgtatataa taaatatctt gctaaaagaa tagatgttta taaaagcgat
      121 aaagaaactt ttacttataa acagtgtagt tctgatttga ccaatttaaa aaaagaatta
      181 aaatggctta aggaacctga caaattttca ctacaaaacg ccttaaaaga tttagataat
      241 gcatataaaa aattctttaa agaaaaggca ggatttccta aatttaaatc aaagaaaatt
      301 aatagatttt catataaaac taactttaca aatggaaaca ttatgtattg tggtcaatat
      361 ataaaactac ctaaacttgg tatggttaag ataagggata aacaagtacc tcaaggtaga
      421 atacttaatg ctactatatc taaagaacca agtggtagat attatgtatc attatgctgt
      481 actgatgtag atattgaagt atttgaaaat actaataatc atataggttt agatttagga
      541 attaaagaat tttgtatctc aagttgtgga gactttatag aaaatcctaa gtatcttaag
      601 aagtcattaa acaagcttgc taaattacaa agagaattat cgagaaaaac aatcggtagt
      661 ttaaatagaa ataaagcaag attaaaagtt gcaggacttc aagaacatat agctaatcaa
      721 agaaatgatt ttctacaaaa actatctact aaattaataa aagagaatga tattatttgc
      781 atagaagatt tacaagtaaa gaatatgatt agaaatcgta aactatctcg cttaatttca
      841 gatgtatcat ggtcagaatt tattcgtcaa ttagaataca aggctaattg gtatggtaga
      901 aaaattgtaa aggtaggtaa attctttgca agttcacaaa tatgcaataa atgtgggtat
      961 aaaaacgagg aagttaagga tttaaatata agagaatgga tttgtcctag ttgtaatgaa
     1021 actcacgata gagatataaa tgcaagtata aatatattaa aagaaggatt aagactaata
     1081 acgattcaaa ataaataact tatgtaagaa ccgtaggaac tacggggata gcctgtggag
     1141 aattagtaag acatatttta atatgcaaaa ttctgtgaag caggaaccct gtgactttag
     1201 tcatgggagg ttcagtcaaa ggtatctagt atagttttaa ctcaagacat gatgaataga
     1261 aataatatta gctttaatga tgttgatgga ataacaacat atacaagaga cataaaaggt
     1321 atagaagttg gaattttatt taaagagaaa aaacaaaatg aaattaaagt gagttttagg
     1381 tcaaaaaatt atgttgatgt aagtgaaata gccaagttat ttggtggtgg tggtcatgta
     1441 agggctgctg gatgtactat tagagatagt attgataatg caaagaaaat ggtactagag
     1501 gcagtgttaa aatcaattta aggtgacagt aaaatgaata agattataag tatattgaaa
     1561 cccacaggta tgacatctca tgatgttgta agtagagtga gaaagatttt aaatataaaa
     1621 aaagttggtc atacaggtac tcttgaccca gatgcttcag gtgttttacc aatctgtata
     1681 gggaaagcta caaaagtatg tgaagttatt ttaaacaaag ataaatcgta tatatgtgag
     1741 cttactttag gtataagtac agatacttat gatgcttcag gtgaaatttt aaaaaaagtt
     1801 gatgatttta aatttagtaa tgaagacata gaaagagctt ttgacacaca aagaggagag
     1861 ataaatcaac ttcctccaat ttattcagct ttaaaggtaa atggaaagag aatgtgtgat
     1921 ttagtaagaa gtggtagaca atctgaaatt actttaaaaa caagaagagt aaatataaaa
     1981 gacataaaaa tactaagtat aaaaggtaat aaagtaatgt tttatgttga gtgttctaaa
     2041 gggacttatg tgagaagcat ttgccatgat ataggagaat atctaggttg tggagctcat
     2101 atgtcgtttt tgaatagaac atcttctggt aaatttgact tagataattc aataacatta
     2161 gaagaattag aattatttta tgaaaataaa actttagaca aatacttata tgatatagat
     2221 tatgtccttg attcgtttaa ttatgttgta ttaaacccta atgctataaa atattatagt
     2281 aatggtggct caatagatga taaaaggttt ttaaaaaata atttcgataa agatgatgaa
     2341 tttgtaagag tatacagtac agataatttc ttaggattag gaaaattatc aaaacacaat
     2401 aatacaataa gtgttaaaag tgataagatg ttcatataac caatgtattt aaaaaaacaa
     2461 caaaagagat agtactataa taacttctat agtctatctc ttttacttat gctataatag
     2521 tttgtgaata agttaagagt aaattatatt ttagcgggga acatttatta tggtgattat
     2581 aaaatctata aaagaaatag caaacataaa agaaagtgtc attaccatag gaaattttga
     2641 tggtgtacac aaaggacatc aagttcttat aggaaaaaca gttgaacatg caaaaaaaga
     2701 aaatatacag agtatcgtat ttacatttgc aaatcatcct gtaaattact ttagaccaaa
     2761 ctcaacaaaa aatattataa gcaatgatga taaaataaaa ttattagaag atttgggaat
     2821 agatatagtt gtaatcatac catttgatga gtatatgact aaaatacctg caaaggattt
     2881 cgtagatgag atactagtaa aaaaattaaa agctaaaaag atagtaattg gacatgactt
     2941 tacatttgca agggcaaaag aaggcaatgt taatctatta aaaagcttag aacatgaatt
     3001 tggttttgag gtagaagtaa taaaacccat caaaatcaat gatgtaagag tgagtagtac
     3061 ttatattaga agtttagtat cccagggtga tatggctaat gtcaaggaat atttgggtag
     3121 aaattacaag ttagaaggtt gtgttataca ttctaaacac cttggaagaa ctataggatt
     3181 cccaacggca aatattgacc ttaagaataa tatgctcgtt ccaaagagag gtatttatgc
     3241 ttctattgta catattggag atgagactta ttttggtgct acaaatgtag gctataaccc
     3301 aacagttaat ggaaaaactt tgtctattga aacaaatatt ttagagtttg atagagatat
     3361 ttatggggaa aatataattg ttgaattttt ggaaagaata agagatgaaa gaaagtttaa
     3421 ttcaatagat gacttaaaaa atcaattgta taatgataca aattatgtgt atgaaaacta
     3481 tgtttgcaaa aacaagtaat gtatgataaa atgattaagg aaaataacca ttactcagca
     3541 tttgaatact ccaatcaatg cttagtaata ggcgtttcta aaaaaatacg gaggtaatct
     3601 aaatgataaa caaatcagaa ataataaaag aatatggaag agctgaaggg gatactggtt
     3661 ctccagaagt acaaatagct ttattaacag ctagaataaa cgagttaaat ggacatttaa
     3721 aaactcataa gaaagaccac cattcaagaa gaggactatt aaaaatggtt ggtagaagaa
     3781 gaaacttatt agcttactta aaggaaaaag atcttgaagg atatagagca ttaatagcta
     3841 aattaggatt aagaaagtaa tttcattctt tataaaaaag caggtatagt gtatacctgc
     3901 ttttttatag ttaattcatt ataaagtaaa atagttaata ataaaagtag tttatatgtt
     3961 ataaaaaatt ataaaatgaa taatattggg taagaatatt aaaatagtat acaaatacat
     4021 aatcaattta aaaggaaaga caggataaat atagaaattt tataacagga taattagttg
     4081 gaataaggag gatattattt tgaaaataaa gttagtatgc gatagtttgt gcgatattcc
     4141 agatgaaata tcagaaaaag attatataga gatagttcca cttacagtta tttttaatga
     4201 tagggaatat atagaaggag tagatataaa gaaagaagag ttttataaaa aagtaaaaga
     4261 aatcaagcaa atacctaaaa cttcacaagc tacatatatg gagtttaaag aagtgtttga
     4321 taagttcata acagaaggtt atcatataat ctgtatgaca ggggctgcaa acgcatcagg
     4381 aacttttcaa agtgctatga tagctaaaaa tgatgtaaat gaaaatgaaa aaatacatat
     4441 atttgatact agaaatttat ctctaggtag tggacaatat gttataaagg catgtgaact
     4501 tcttgaagaa ggactgggat ttgaagaaat tatagatgaa ttagaaaata ctcgcagtag
     4561 tgtaagattg ctttttgcac catatacact tgatttttta aagcagagtg gaagagttcc
     4621 agttgcaact gctttgatag gaaatatgtt aaatataaag cctatatttt tctttgataa
     4681 tggggaagct aagttggtaa ataaggttag aggaataaag aatatagcaa gtaaattggt
     4741 cgatataatt ttagaaatga atgaagggtc tttagaagga aaaatcgtca caataggttg
     4801 tggagataat ttacacgatt gtgaaatatt aaaagatgaa gtaaataaaa gaataaaagc
     4861 aagaagagta ttatttactc gaggaggagt ttctatttgc tctcatacag ggccagatat
     4921 attggctata agttgttcta attagtagta atataaggtt tttgcatata tatgtgaaga
     4981 taattttata ttgttaattt atagtatttt tgataatata gtaaatagag tgatatacgt
     5041 gttttttaat aagaggagga caagcattaa tgtttgaaca taaaatattt aaaatggatt
     5101 ttgcaggaag agaactttct gtagaaatag gaaagatttg tgaaatggct agtggtagtt
     5161 gtatagttcg atacagtgat agtatggtaa tggtaaatac aactaaatca gccaaaccta
     5221 gagacggaat agactttttt cctttaagtg tggattatga agaaaaatta tactctgtag
     5281 gtaagatacc tggaggtttt ttaaagagag aaggcaagcc ttcagagaaa gctatactta
     5341 catcaagatt gatagataga ccaataagac ctttattccc aaaaggattt agaaatgatg
     5401 ttcaggtagt tgcaacagtt ctatcagtag accaagattg tacaccagat atagtagcaa
     5461 tgataggttc ttcaatagcc ctttctatat cagacatacc atttaatgga ccaacaggtt
     5521 cagtatgtgt tggccttgta gatggtgcat ttgttgtaaa tccaaacgca gagcaaagag
     5581 aaaaaagttc aatgcatcta gttgtttcag gtacaaagga agcaataatg atggttgaag
     5641 ccggagctga tgaagttcca gacgaggtaa tgcttgatgc tattttattt gctcaccaag
     5701 aaataaaaaa aatagttgag tttatagaag gtatagttgc agaagttgga aaagaaaaaa
     5761 tgcctgttga attataccat gcaggagaag aaataactca attggttcgc gaatttgcaa
     5821 ctgataagat gaaaaaagca gttcagactt ttgaaaagtt agagagaatg gaaaatatgg
     5881 atagagttaa agaagaaact ttagctcact ttgaagaaac tttagaagat tttgaagatt
     5941 ttgttggaga tatagaagaa gtattacaag atataataaa agaagaagta agaaaactta
     6001 tagtacatga aaatgtaaga ccagataata gaaaacttga agaaataaga cctatatggt
     6061 gtgaaactgg catgatacct agagctcatg gttcagccat atttactaga ggacaaactc
     6121 aagtacttaa tgttgctact ttaggtgctt taggagatgt tcaaaaacta gatgggttag
     6181 atgaagaaga aaacaagaga tatatgcatc attataattt ccctgcatat agtgttggag
     6241 aagctagacc atcaagagga ccaggaagaa gagaaatagg acatggtgct ttagcagaaa
     6301 gagctttatt accagtaata ccatctcaag aagaatttcc ttatgctatt agattagttt
     6361 ctgaagtatt aagttcaaat ggttctacat ctcaagcaag tgtatgtggc tcaacattat
     6421 ctttacttga tgcaggtgta cctataaaag atatggttgc aggtatagca atgggactta
     6481 taaagcatga tggtaaggtt gcagtgcttt cagatataca aggtatggaa gaccatttag
     6541 gcgatatgga ctttaaagta gctggaacag agtatggtat aacagcaata caaatggata
     6601 taaaaattga tggtatagat aaagaaattc tacaacgtgc tttaaaacaa gctaaagagg
     6661 gtagaatcca tatactagga gagatgagaa aaactatatc tcagccaaaa ccagaacttt
     6721 caccatatgc acctaagatt gttaaaatgc aaataaatcc tgacaagata aaagatgtaa
     6781 taggacctgg tggaaagata ataactaaga taatagacga aactggagtt aagatagata
     6841 ttgaacaaac tggggaagta tttatatcag gtattgagat tgatatgatt aagaaagctc
     6901 aagaacttat aaataatata gtagttgaac cagaagtagg aaaaacttat aaaggtaagg
     6961 taagtagaat aatgaacttt ggagcatttg ttgagatact tccaggaaaa gaaggattac
     7021 ttcatatatc tcatatagct catgaaagag ttgccaaagt tgaagatgta ttgaatatag
     7081 gggatgaagt tgaagttaaa gttacagaga ttgatgaaaa aggaagagtt aatttatcaa
     7141 gaaaagttct tttaccaaaa ccagaacata aaaataaata acttattata taattaaaag
     7201 caaagtctta ggattttgct tttttaaata caaaaaagta gataatttta aaatgatagt
     7261 tatggatggc ttatagttct ttttctgtca ggctttactc ttataaagaa gctatttaaa
     7321 agctgattaa tagagagctt tattgatata tatagaatca cttaagttat atttagtgca
     7381 taaacagagc ttatttttaa tagtattaaa taagaaacaa tttgtacata attaggggga
     7441 gttttaatga taatgatggt acttaataag agtaagctca aaaaaatcat aatactaatt
     7501 ttagtagtgt tattagtatt atcaggaggg gtaatggtat taaagttatt taacaacaag
     7561 ccagtattta atttatttaa tggtgtagat ttaccatatg aaagaggtac aaaggataaa
     7621 agtggatatg ttgcattaac ctgtaatata gaccttggat gggaaacaga gtacgtagaa
     7681 tccattttag aaacacttaa aaaagaaaat gtaaagatta catttaatgt aactggaaaa
     7741 tgggcagaaa agaataagga tgaattatta aagataaaaa aacaaggaca tgaaataggt
     7801 aatcatggat acaaacattt agattattct acattatcat atgaagacaa ttatgagcaa
     7861 atagagacct ctaaaaagat aattgaagaa attataggtg aaaaaactaa attttttcaa
     7921 gcaccagcag gttcttttgg tccagaaaca gtaaaggctg ctaaagcatt agggtatact
     7981 tctatcaaat gggatgccga tactatagat tggaaataca aagaccaacc agaggtaatc
     8041 attgatagaa tgaagaaaaa agacattaaa gatagtagta taattttaat gcatccaacc
     8101 aacgcaacga caaaatgtat agatgatatt atagctattg taagagaaaa aggactaaaa
     8161 ccaggaaaac ttagtgatgt atttaagtag acaaagtata aatatttggt taagattgta
     8221 taagttgtag ttgaattaat taaaagactg acttataatg taatacatat aaaaaataaa
     8281 tataattaaa acatagtgtt ttcaataatc caatatgaaa caaagaataa attgttagga
     8341 gaatgtaatg tataagacaa aaatactgga aaatgggctt acaataattg gtgaagaaat
     8401 accatatcta aagtcaataa cattaggtat ttggataaat gcaggctcta ggatagaaga
     8461 agcacaggtg agtggaattt ctcattttat agagcatatg atgtttaaag gaaccaaaaa
     8521 cagaacatca aaagagattg ctagttcaat agataatttg ggtggtcaaa taaatgcatt
     8581 tacaagtaaa gaatgtacat gttattatgt aaaactaata gatgagcata tagatacagg
     8641 aattgatgta ttgagtgata tgatactaaa ttctaaattt gataaaaatg atatagataa
     8701 ggaaagactt ataatattgg aagaacttaa gatgtatgaa gactctccag atgacttatc
     8761 ttatgattta ttagtggaaa atatatatgc taatgatgga ttaggaatga atataattgg
     8821 gacaaaagaa tctctgtaca atataactag agaatccatg ttagaatact taaataagta
     8881 ttatattcca aataacgctg ttatttctat agctggaaac tttaactttg atgatatggt
     8941 ggagaaaatc aaatcaaaat ttggacactg ggaaaagaaa aatttaagta tagacataag
     9001 tgaggctaag tttaatcctt gctttatatc aaaaaataaa gatactgaac aggtgaactt
     9061 agctatgtgc ctaaaaggta ttccttttga aaatgatgaa gaagtctatt caatggcagt
     9121 ggtaaataat attttcggtg gaagtataag ctcaaggctt tttcagaaaa taagggaaga
     9181 aaaaggttta gtatattcaa tatactcatc acagacatta tataggaaat gtggtgaatt
     9241 aggtatattt gcaagcatga gtacagaaaa tttacaagat gtatataatt taataaagaa
     9301 agaaattgaa aatataagag aaaactatct tacagaaaaa gaaatatctg aaagcaagga
     9361 acagttaaaa ggaaattata tattggattt agaaagtact agcagtagaa tgatgtctac
     9421 tggtaaatct atgttactta gcaaaaaagt aaaaactaca gatgaaatac ttgaatgtat
     9481 aaataatgtt aatattaatt caataaagaa ggttgttgat aaggtattca atatcgaaaa
     9541 tattggtaca tgtatagttg gaagagatgt agaaaagatt ctacacttgg attaatattg
     9601 aaaaaagtaa atctctggag gtataaaata tgaatttatc tgaaatagca ggtaaggaaa
     9661 tagttaactt ggtcacagga gaaagattgg gggtcattgg agaatgtgac cttatactag
     9721 atgaaactac aggcgatata ttagcgcttc taattccaaa agaaaaagga ttttttgcca
     9781 gaagaaaaga taagtcattt ttagaagtac catggagaaa tgtaaaaaaa ataggtaatg
     9841 acatgataat tatagaatat gagggatata tttaggagga aaaaatgagt attttagtgc
     9901 aaaagtttgg tgggacatca gtagaatcat atgaaaaaat gaatgaagta tgtaagatag
     9961 taaaagcata caaaaaaaat gatgaagaat tacaattagt attagtagtt tcagcaatgg
    10021 gaagaaaagg agctccatat gctacagata cacttataaa tttgtgtagt gcagtaaatg
    10081 atgagccaag taaaagagaa cttgatttaa taatgtcttg tggagaaata atatcaggaa
    10141 caattcttgc taatttattg aatgcacagg ggatagattc tgttttcttt actggtcagc
    10201 aagcaggtat aattacaagt gatgaatatt ctaatgcaaa gataaagtat ataaatccta
    10261 aaaaggtaaa aagagcttta gatgatggta aggttgtagt tatagctggg ttccagggtg
    10321 taacagatga tggagaaata actactcttg gaagaggcgg aagcgataca tcagcagttg
    10381 ctattggtaa agctttagaa tgtgaaacag ttgaaattta cactgatgtg gatggtataa
    10441 tgactgcaga ccctagagta gaaccaaacg ctaaagtatt gagttttata gactatgagg
    10501 aagtattcca aatggctgac aaaggtgcaa aagtaataca tccaagagca gtacagttgg
    10561 ctaaaagtgg aaatataact ttagccataa agaatacaat gaatccaact tttgaaggta
    10621 caaaaatagg ttctttatgt agacacttag aagacaatat agaatatgaa caagaaagag
    10681 attttaaggt tgctgttgca aataaagata gcgtagcaca agttaagatt aagtcagcag
    10741 aagaagtgtt tacagaagta ttaaatgaga tagaaaagaa acttataact atagatatga
    10801 taaatttctt tatatcagaa aaagcatttg ttgtagaaga tgctgatata aaatcattaa
    10861 aggaaatact agataaattt gaattagatt atgaagtaaa tagagactgt gctaaggtaa
    10921 cattgatatg ttcaagaatt gctgatgaaa tgtctggtat catgtcaaag gtagttagag
    10981 gattatcaaa agcaggggta tctcttttac aaacatcaga ctcaaatatg actatatctt
    11041 gtttagttag tgaagaagat atgcatacag cagtacatgc tatacaccaa caattttatt
    11101 taaaataaac tgtataatac aattattttt taatcaaatt taagaatttc atatatatct
    11161 taatatattt gatttatttg gtaaaactaa attaaatata attttgatag gagttttttt
    11221 atgaaagatt atagagaaga taatgaaaaa tttgataaag ataattgtag tgaagaatct
    11281 agttgcgaag agagagaatg tataaagcaa tttggtacca atgaaatgcc acctcaacct
    11341 cctaaagata ttcaatgtat aactataatt ggagaaattg agggacattt tataggaaat
    11401 cctcaaaaaa aagctacaaa gtacgagcat ataattccaa tgctatactc aattgaggaa
    11461 agtaatgatg ttaaaggagt gttagttgtt ttaaacactg ttggaggaga tattgaagca
    11521 ggtttagcta ttgcagaact tttaaacagt acatctaaaa aagtagttac tcttgttcta
    11581 ggaggaagtc atagtatagg tgttcctctt gctacagcag gagattactc atttatagca
    11641 ccaacagcaa ccatgattat acatccagtg cgaactactg gtcttgtgat tggtataaat
    11701 gagacttttg aatattttaa aaagatgcaa gatagaataa ttcaatttat aataagaaca
    11761 tcaaatatca aaaaagatgt acttgaaaaa cttatgcatg aaaaagatga acttgtaagt
    11821 gatgttggta gtgtattaat aggaaaagaa gctgtggatt atggtcttat agatgaagtt
    11881 ggaggactaa aagaagcact taaaaaatta agagagctta taaaagaatc tgaggaagaa
    11941 aaaaataatg attaaaattt gtttaaataa taaaatttat gatataatga tgtaataaag
    12001 taggagtgtt agtgttaagg cattagcact ttttgctgtg aaggggtaaa acttgctaaa
    12061 aataaggagt gatattaatg gccacgaaaa agaagaaaaa aaagaaggta aataccttaa
    12121 gttttaatgc agaatatcat aatttaataa caatatttat aggtgtattt ttattatata
    12181 gtttaaattc taattctatg ggatggatac ctgtgttaat gcaaaatctt tttaagggac
    12241 tatttggagg gctttcaata gcaataccat ttatagtaat tataacagga cttttaggat
    12301 tttttgatgg aaatgaatat atatataggc taagaaagac taaactatac tacataatta
    12361 ttttatttat atttgtattt tatggattat taaatgcagg aactcttcct gtagatagcc
    12421 cactaaaagg taacatgttt gatgatgtta tgaaattggg tgtaagtgga caaggtagtg
    12481 gtttgatagc aacaactatt gcgtattata tgagtaaaat atttggtata gcaggtggat
    12541 ggttaataag tatcttcgca cttatacttt ctgtaatgtt tatatttaat atttctataa
    12601 aagatttggt gtcaaatgca aaatcaaaat ctaaagcatc taaagacagc aatttaacgt
    12661 ttaaagataa aattgcaaat atgaaaaaat ctgcaataga tatgatgact gatgaagtag
    12721 atgatactac tataaataaa aaaccaggtt tctttaaagg gttaatgtca aaaggtctta
    12781 ataatgatga tgaagaagat gaatattttg aagaagaaaa tagtgatggt gtagatgata
    12841 aaactataaa aatagttgga tttaataagg cagaagatga gtatttagaa atattagaag
    12901 gaactcaaag tatgccagag ttagatgttt taaaagaatt acaaaaagct accaatgaaa
    12961 atccagttgt agatactaaa ccagaaaaaa aagttgatat agctaaacct aatttaaata
    13021 tagaaaaaac tcaacctatg agtattgtag cagagccagt taatgaggat tattcaaact
    13081 ataaaaagcc tagcattgag cttttaaata aagtaaataa gaagtctgat gaaaatggta
    13141 aaaagaaagt gttgaaaaat gcttctcttt tagaaaagac actatcagat tttggagtgg
    13201 aagcaaaaat taatcaagtt acagttggac ctactataac aagatatgaa atacaaccaa
    13261 gtccaggagt caaggtaagt aaaattgtaa atttaactga tgatatagct ttaagtttag
    13321 cagctaaaag tataagaatt gaagccccaa ttcctggtaa aagtgcaatt ggaatagaag
    13381 ttccaaatga agaagctcaa atggttggtg tgagagaagt tctagaaagt gaagaattta
    13441 ataattttaa ctctcctctt gcaatgggac ttggaaaaga cgtagcagga aaaattataa
    13501 ttggagatat aggtaagatg ccacacttac taatagctgg ttctacaggt tcaggaaaga
    13561 gtgtgtgtgt aaatacatta ataagtagta ttttatataa ggcaaatcct gatgaagtaa
    13621 aattactatt aatagaccca aaagtagttg aacttgcaaa ttataatgga ataccacatc
    13681 tattgatacc agtagttact gaccctaaaa aagctgcaaa tgctttaaat tgggcagtta
    13741 cagagatgaa tagaaggtac aaattatttg cagatgctca agtaaaagat gtaacaagtt
    13801 ataatgagaa agcggaagaa aaacttccta aaatagtaat aataatagat gagttagcag
    13861 atttaatgat ggcaagtgca aatgatgtag aagattatat ttgtagatta gctcaaatgg
    13921 caagagctgc tggaatgcac ttgattgtag caactcaaag accatctgtt gacgttataa
    13981 caggggtaat aaaggctaat ataccatcta gaatagcatt tgcagtatca tctcaaactg
    14041 attcaagaac aatccttgat atgggtggtg cagagaagct tctaggtaaa ggtgatatgt
    14101 tattctatcc acttggtgca gcaaaaccag ttagacttca aggggcattc atatcagaga
    14161 gtgagtcgga aaaagtaatc gattttgtaa aaagtcaggt taaagatgga ataaaatatg
    14221 aagaagatat aattgaaact atatctaagg ttaatacttc aaagggtagt gatgaggatg
    14281 agtttttgag tgaagcgata gaatttgtcg tggaaagtgg tcaagcatct gcctctatgc
    14341 tacagagaag atttaaaata ggattcaata gagcagctag acttatagat tctatggaag
    14401 aaagaggaat aattggagca agtgagggaa gtaaaccgag aaaggtatta ataagcaaac
    14461 aggatttgca gaatttagaa ggtgaataga attgtcagtt ataaaaatag aaaaagcact
    14521 taagctagat aaagtaatat ttgtagatgt gagaactgaa ggagaatatg aagaagacca
    14581 tatattgaat gcaatcaata tgcctctttt taaaaataat gaacataatg aagttggaac
    14641 aatttataag atgcagggaa aacatgaagc aatacaaaaa ggatttgact atgtatcgta
    14701 taaattaaag gatatatatt tacaagcagc agaacttgca ttaaattatg ataatattgt
    14761 catatattgt gcaagaggcg gaatgagaag tggcagtata gtaaatttat tatcatcatt
    14821 aggtgtaaat gtatatcaac ttgaaggtgg atataaggct tatagaaatt ttgttttaga
    14881 gtatttaagg catgtaatgg atacaaaaaa ctttgtatct gttcatggat tgactggtac
    14941 aggaaagact gatttgttac atttattaga tgaaaaagga atagacgtgc tggatttaga
    15001 aggcatagct aaaaacagtg gttctacctt tggttttata acttttgata aaaagccacc
    15061 atctcaaaag aactttgaaa caaaactatt tgaaagtata tttttttcga aagagaatta
    15121 tatatttgtc gagagtgaaa gcaaacgagt tggaagtgtt acagttccaa atgaaatttt
    15181 cgaggcaatg acacgagaag gacatcatat attattagaa tgtgatattg aaaatagagt
    15241 tgatagactt tgtagagatt atatttatga taaagatgaa ggtaatatat tgattttaaa
    15301 agaatgtata aacaagttta gaaaaagatt gggtaataaa gtagtagatg attatattga
    15361 tttattagaa tcaggtgaat ataaagaact tgtaaaaaga tatcttttag aatactacga
    15421 cccactttat atgcattcag tagagaaata taagtatagt aagattataa attttaatga
    15481 tacacaaaaa gctttagaag atgttataga cttatatgag agtatattgg aaggagaaag
    15541 tgaatgttaa aaatagcatt ggaatcatta ggatgctcaa aaaatttagt agatgcagaa
    15601 ataatgatgg gaattttaaa taacaaaggc tataaattaa ttggagattt tgaagaagct
    15661 gatgtaatta tagtaaatac atgtgggttt atagagtcag ctaaacaaga atctatagat
    15721 acgataataa actttgcaga gttaaaaaaa actggcaatt tgaagttgtt gatagtaaca
    15781 gggtgtctag cccaaagata ttctgaagaa ttaaaaactg aaattcctga aatagatgct
    15841 atagtaggaa caggaagcta tcaaaatata gataaaatac ttaaagaatt aagtgaaatg
    15901 catcaaattg taagccttaa tgatatagag tttgtattta atgaagattt accaagatat
    15961 atatctacac ctagctatat ggcctattta aaaattggag aaggatgttc caataattgt
    16021 acttattgta taataccaaa actaagaggt aaatatagaa gtagaaaatt tgaagatata
    16081 attaaagaag ctaagaaatt agctgaatct ggagtaaaag aacttgtggt tatagcacag
    16141 gatacgacaa agtatggttt tgatttatat ggaaaagaaa gattatcaga acttttagaa
    16201 gaacttgcaa aaatagatgg atttaagtgg ataagggtta tgtattcata tccagaatct
    16261 ataacagaag aattgataca agttataaag aaatatgata atatatgtag ttactttgat
    16321 atgccaatac agcatgcaag taacaatatt ttaaaattaa tgaatagaaa aactactaaa
    16381 gaagatatat taaataaaat caatctgata agaagtaata taccagatgc tatacttaga
    16441 actacaataa tagtagggtt tcctggtgaa acagaagatg acttcaaaca acttgtggac
    16501 tttgttgaag aggttaaatt tgatagactt ggtgcatttg catattcaag agaagaagat
    16561 acaccagcag atagattgcc aaaccatata gatgaagaag taaaaataca aagaagagat
    16621 actttaatga tgattcaaca aaaaatatct gaagaactta atgacaaaaa gataggaaaa
    16681 acttatgagg tgttaataga agaacagata gaagataatg tatatacagg aagaacacaa
    16741 ggtgatgcag aagaaattga cagtatagtg tatgtaaaaa gtgtagacaa tcttgaagta
    16801 ggagaatttg taagtgttca aataaatgat gcaatggaat acgatttaat gggagatgtt
    16861 ttatatgaac ttgcctaaca agttaacttt gtttagaata tttttaatac cagtatttgt
    16921 tttaattatg ttgctcaatg taccaaataa atttttaata gcttgtatta tttttataat
    16981 agcttcaatt actgatgctt tagatggaaa aatagctaga aaatacaatc tagtcactga
    17041 ctttggaaaa tttatggacc cattagcaga taagttattg gttatttcag cacttacatg
    17101 tatgatagaa gaccatcttg taagtagttg gatggtaatt ataatagtgg ctagagagct
    17161 tacagttagc atattaagag ctatagctgc tgctgatgga aaagtaattg ctgctggaaa
    17221 cagtggaaaa cttaaaacga taactcaaat ggtatctata gtgtttttat tgttaggtgc
    17281 tcagtttgaa aatgtattga tattaaatat tggtgaaatt ctcattctta tagctacact
    17341 acttacatta tattctggat gggaatatct atataaaaat aaagagcttt ttatgtcttc
    17401 taagtaaaaa attatgcttt aataaataaa aactaatgat taataaataa aaaataacaa
    17461 cttaaaattt taaaaatgta aaaaagggta tactattata gtatacctct tttcatttta
    17521 agaatatttt cttttgtgta tataattatt aataatggtt tataaataaa atgtaatgaa
    17581 taaaaatata atcattagta gcgtttttaa attgactagg tttgatttat gttatataat
    17641 taatatataa caagagaaca aatgtttgta gataaatgaa gggatggtat agagatgagt
    17701 gtagatcaag aaaaattaaa agcgttgaat gaagctttag gtaaaattga aaaagatttt
    17761 ggtaaaggtt cagtaatgaa attgggagaa gcaacatcta tgtctataga tgttatatca
    17821 acaggagcga ttggtttaga catagctatt ggtataggag gtctacctag agggagaata
    17881 gttgaagtat atggtccaga atcttctggt aaaactactg ttgcgcttag ttgtgtagca
    17941 tcagctcaaa aagatggagg aatagctgca tttatagatg cagaacatgc acttgaccca
    18001 gtatatgcaa aagctttggg tgtggatgtt gataacctaa taatatctca accagataca
    18061 ggtgaacagg ctttagagat agcagaggca ttgataagaa gtggagcgat agatataata
    18121 gtaatagact cagtagcagc attagttcca aaggctgaaa tagatggaga tatgggtgat
    18181 tctcatgtag gtttacaagc tagacttatg tcacaagcac ttagaaagtt aactggttca
    18241 attaaaaaat caaattgtgt tgctatattt ataaaccagt taagagagaa agtaggaata
    18301 atgtttggaa acccggaaac tactactgga ggacgtgcac taaaattcta ttcatcagtt
    18361 agattggatg ttagaaaaat agatacaata aaacaaggtg ataaagttat aggtagtaga
    18421 actagagtta aagttgttaa aaacaaagta gcaccaccat ttaagcaagc tgaatttgat
    18481 ataatgtatg gagaaggaat ttcaaaaatt ggagaccttt tagatatagc tgctgatgta
    18541 gatatagtta aaaaatcagg ttcatggtat agttacaatg atactaaact tggacaagga
    18601 agagaaaatg ttaaaaaatt cttggaagat aatttagatt taactactga aatagatgag
    18661 aaagttagag cattttacaa tttaaatgaa gaacatgaag aatcaggtat ttcagtatca
    18721 aaagaaattg tagaagaata agtaaaaata caaaaaaata actagtaatt aaagacatgc
    18781 cacgctccct attagcttga cacaatgttg aaaaaattat aaaattaagt gaggataaaa
    18841 tatctaaatt taattttaat tattaggaat attttttaat catagtgatg acattttggg
    18901 attattaacg aaggaggtgg atgtcataga tagtgtagta atgattgtaa taggtgcagc
    18961 tgttggtatt atagctggat attttgttag aaaaaatata tctgaagcaa aaattggaca
    19021 agctgaaaac ttggccaaag aaataataga taaagcacac cacgactcag aaacagtaca
    19081 aaaagaaaaa ttactagaag ctaaagaaga gatccataag tggagaacag aagctgaaag
    19141 agaaaacaga gaaagaagaa cagaagtaca aaagtatgaa aaaagagtag tacaaaaaga
    19201 agaagtctta gatagaaaat tacaaaattt agaatctaaa gaagtaaacc taagtgaaaa
    19261 attaaaggta gtggaaaaga aagaagaaga agttgaagta ataaaaactc aacagttaga
    19321 aaaattagaa agtatatcag gaattacttc tgataaggcc aaagaaatca tcttaaccaa
    19381 tgcggagaga gatgtaagac gtgaaatgtc tataatgata aaagagatag aaagtcaagc
    19441 taaagaagaa gctgataaga aatcaagaga aattatagga tatgcaattc aaaaatgtgc
    19501 agcagatcac gtagccgaaa ctactgtaac agtagtaagc ttgcctaatg atgaaatgaa
    19561 aggtagaata ataggtaggg aaggtagaaa tataaggact ttagaaacct taacaggtat
    19621 agaccttata atagatgaca ctccagaagc cgtaatttta tcaggatttg atccaataag
    19681 aagagaaatt gcaagaattg ctctagaaaa attaatagca gatggaagaa tacatccagc
    19741 tagaattgaa gaaatggttg aaaaggcgag aaaagaagtt gacaatatta taaaagaata
    19801 tggtgaacaa gctgcatttg aaactagtgt acatgggatt catccggaat taataagatt
    19861 attaggtaga cttaactata gaactagtta tggacaaaat gtacttaaac attctataga
    19921 agttgctcat atagctggta taatggctgc tgaaatagga gcagatatta gattggctaa
    19981 gagggcaggt ttattacatg atataggtaa ggctgtagac catgaaatgg aaggtacaca
    20041 tgttgaaata ggtatggatt tacttagacg ttataaagag tctaaagaag ttatacatgc
    20101 catgagtact catcatggag attacgagcc tcaaacagta gaggctgtat tagttacagc
    20161 agcagatgca atatcagctg ctagacctgg tgcgagaaga gaaactttag aagcatatat
    20221 aaagagactt gaaaaattag aagaaatagc taattcatat gaaggtgtag aaaaatcatt
    20281 tgctatacaa gctggtagag aaataagaat aatggtaaaa ccagaagcta taaatgatga
    20341 agaaatacat ctattggcta gagatatgac taagaaaata gaggatgaac ttgaatatcc
    20401 tggacaaata aaagtaagca taataagaga aacaagagct atagagtatg cgaagtaaaa
    20461 gaaataatat ctaaataata agaaaatatt tttagaaatt taaatttgcc acaagatatg
    20521 ataatatatc ttgtggcttt taaattgttt aagtttaatt ttatataatg caaaaataat
    20581 ctaagtgcta aaagtgctac t
//
LOCUS       DACULQ010000058        20536 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.058, whole genome shotgun sequence.
ACCESSION   DACULQ010000058 DACULQ010000000
VERSION     DACULQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.058"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..761)
                     /locus_tag="JA154_08510"
     CDS             complement(<1..761)
                     /locus_tag="JA154_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator LicT"
                     /protein_id="HAT7995908.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEK"
     gene            complement(1022..3064)
                     /locus_tag="JA154_08515"
     CDS             complement(1022..3064)
                     /locus_tag="JA154_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAT7995909.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNIMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVIKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATILLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3216..4187)
                     /locus_tag="JA154_08520"
     CDS             complement(3216..4187)
                     /locus_tag="JA154_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT7995910.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKKIKSVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(4188..5165)
                     /locus_tag="JA154_08525"
     CDS             complement(4188..5165)
                     /locus_tag="JA154_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7995911.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5384..6943
                     /locus_tag="JA154_08530"
     CDS             5384..6943
                     /locus_tag="JA154_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7995912.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            7049..8008
                     /locus_tag="JA154_08535"
     CDS             7049..8008
                     /locus_tag="JA154_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7995913.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8011..8823
                     /locus_tag="JA154_08540"
     CDS             8011..8823
                     /locus_tag="JA154_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7995914.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLIDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(9039..9941)
                     /locus_tag="JA154_08545"
     CDS             complement(9039..9941)
                     /locus_tag="JA154_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT7995915.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            10296..11474
                     /locus_tag="JA154_08550"
                     /pseudo
     CDS             10296..11474
                     /locus_tag="JA154_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089187.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
     gene            11483..12139
                     /locus_tag="JA154_08555"
     CDS             11483..12139
                     /locus_tag="JA154_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid dehydrogenase"
                     /protein_id="HAT7995916.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIIEGGNL
                     "
     gene            12139..12804
                     /locus_tag="JA154_08560"
     CDS             12139..12804
                     /locus_tag="JA154_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HAT7995917.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            12909..13685
                     /locus_tag="JA154_08565"
     CDS             12909..13685
                     /locus_tag="JA154_08565"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089190.1"
                     /note="catalyzes the formation of acetoacetate from
                     3-hydroxybutyrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HAT7995918.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            13747..15081
                     /locus_tag="JA154_08570"
     CDS             13747..15081
                     /locus_tag="JA154_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HAT7995919.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            15577..16473
                     /locus_tag="JA154_08575"
     CDS             15577..16473
                     /locus_tag="JA154_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HAT7995920.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PGNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPEDNNNSDSTNKPD
                     DNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKKSQDMIDNNYF
                     SHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGHRKNIMNPNFS
                     KIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(16495..16623)
                     /locus_tag="JA154_08580"
     CDS             complement(16495..16623)
                     /locus_tag="JA154_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995921.1"
                     /translation="MLYTLKLREQIIFIVCIFGFDNFKIDTKEYLICVFYHLEIIF"
     gene            complement(16865..20404)
                     /locus_tag="JA154_08585"
     CDS             complement(16865..20404)
                     /locus_tag="JA154_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAT7995922.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         7078 a         3226 c         2902 g         7330 t
ORIGIN      
        1 ttcttttcta catgagtttt tattttatat gcacaatcat aatccttttc atactttact
       61 ttaattatat ctataatttc attatttgta tcattgattt tttctttttt tactaatcgt
      121 tttgcaaaga attttaaatg agtcagaagc ctatcataat ttatatcatc ttctttaaat
      181 tcaactctat aataatatct tataatattt agtatgtctt ttacaatttt agtaattaat
      241 agtgattcct ttgaacttcc cttatagttt gaatttacta tatgcattgc tataaaacca
      301 gcttcatcta ctggaaattt cacatcaaat tctttattta tataatctac agcccatttc
      361 ccaatctcat attctttttt atgtattctt tttatctcat gtagtaaatc attctttata
      421 gttatgtttt catgatatct tttaattgca aatgcaatat ggtcagtaag tgctacataa
      481 atatattcat aaagcttagt attcaagttc tcctctgcat aagcaattat ttcagtacta
      541 acctcaaaaa atttctcatc aacctggctt gctagtttac taattttatc taaaaagctt
      601 tcatcttgta ttacaaaagt tctttcaatt ttacttttat ctatttcttg acctttcttt
      661 ttttgaaacc caattccaca tcctgttaat attaattctt tttttatatt ttcatctaaa
      721 actaatacaa cattattatt ataaatttgt tgaataatca taatcaatat ctctcccaaa
      781 atacaatttc ccttattcat aaaaaaatac ctaaattaag atacaattca cctgtatacc
      841 ttaacctagg tattgcctgc ttaccagtaa caatccagct tgttaatgct tatatattgt
      901 attcaattta tggttttatt ctattataaa taaatatata tgtcaagtaa attattgaaa
      961 actatttcct attttttagt attaatttta acatcaaaaa tcaaatttta ttccattata
     1021 tttatgattc tatcatcatc atttcatcat agagaatatt ttccaataac tctttagcca
     1081 cttctcttga tatttgctta gtttcaacta attgttgaac actgtctctt tgactttgta
     1141 tcgccattac tctgtagtat tctactcttg cctcaaactt atcatcattt tgaatctttc
     1201 tagtaatcca attcaaataa cgattataga atctgattgc tattaaggcc tcatgtctgt
     1261 tatcttcatc agtattttta tttataaatg atatagcagt tttagtagaa agtgcatatg
     1321 catggtgaaa ctctttgatt atttctttat tctcagatac tatttttgta attttttctt
     1381 ttacttcttt ctttactttt ctatttataa tccacatgct tatttttaat tttacaattt
     1441 taaatataga tttttgaaca gattttcttg attttgtaat ataaaatcta tacaatttaa
     1501 aggctctatc agaaatttta ccatcttcta atagtttcaa aacagctaat aatccctgtt
     1561 ggtctgcttc tgcaatcatc atacttataa gtttattatc tggagtattt agtattcctt
     1621 tttctgagtc taccaactgt tcatttaaat catgtataac tggttgtact tcttgtgctt
     1681 tttcattact aagttttaaa atggcttctt tcaaaattag tttatatgcc tctttatcag
     1741 ataataaagg ttctgactct tgtacgttat tttttaataa tcttggcagt aaaattgttg
     1801 ccaatactaa acttataaga ataacttctg atgatataaa taaaacagta tctctcattg
     1861 gaaatacatc tttatcagcc aaataaaaag gtatagataa ggcagttgct agtgtaaatg
     1921 taccatgaac accacaagta gcaactatta gtgagtattt acatttagaa atactctctt
     1981 ttataacttc ttcatcctta aaacctaaaa aacctacaaa aaaattattt aaaggattct
     2041 ttttaggttt tataaaagaa ttatgccata aatataccca tataaatctt ataacaaata
     2101 aaataagtac aattaaagta gaaataaaag tcaattccaa tgccatattt tcatttttac
     2161 tactaagtcc tgtatatata cttggtaaca aaaaaccaag cagtgtaaag acaaatccat
     2221 tcaatacata ctccaaaaca taccaagtat tgtctgatat tatccttaat ttagtagttg
     2281 tattttgtaa atgctctttt tcaattccat gagctatccc tgccattaca acagctagga
     2341 ttccagatac tcctatttct tctgctacaa aatatacaaa aagtggagtc attatttgta
     2401 taactattac cattggaatt tcttccaaat tccacttgtg taaactaagt cttattttta
     2461 taattatgta tcctaaaata cttcctacta ttattcctcc aaaagctgta atcataaatt
     2521 gaatccctgc ctcttctatt gaaaaaactc ctgtaactgt agcaagtact gcaaccttaa
     2581 atgctactac tccagcagca tcatttaata aagattcacc ttcaagtata ctcattatat
     2641 ttttaggaaa atttaatcct tgtgtaattg attttactgc tactaaatca gttggagata
     2701 ttactgctgc caaagcaaat gaaacagata atggcatccc aggaataaga aaatgtataa
     2761 agattccacc tgcaaacacg gttattaaaa ctaaaccaaa tgcaagaagc aaaattggtt
     2821 ttctaagctc taatagctct tttctcgata cattttgtcc ttcactgaat agtaatggag
     2881 ctatgataca tattacaaat atttctggat taaatgaaaa tgagaattta aatggcaaaa
     2941 tagacaatat aatccctagt acaatttgat atattgcaag aggaatatta ctccactttt
     3001 tgctaaatac agttgaaatc gttgtacaaa ccaataatcc caacaatgtt actaaaattg
     3061 acataactac ctctcttttc aataataata ttatagatta aacttatata tcaactaact
     3121 taatattaac agcatgaaat cccatcttaa tcaaaaaaat tttcagttta ttctattatt
     3181 cttattatat acatcaaatt taatttttga aatatctata ctagatgaca tgcaacaaaa
     3241 tgatttcttg atatttcttt taaaactgga atctcttttt tgcatatttc cttacaataa
     3301 gggcatcttg tatgaaactt acatccactt ggtggatttg ttggagatgg tatgtctcct
     3361 tttaatataa ttctattttt cttaattgta gggtcttgta ttggtactgc gctcataagt
     3421 gcttttgtat atggatgttg agaatgtaag taaagttctc ctttagttgc aatttctatt
     3481 atagaaccta gatacataac acaaactcta tgtgcaatat gatttactac actcaaatca
     3541 tgagatataa ataaatatga aagtttatat ttatcctgta aatccataag taaatttata
     3601 atttgagatt gaatagaaac atctagtgca gaaacaggtt catcacaaac tataaattct
     3661 ggatttaatg ctaatgctct tgctatacct attctctgtc tttgacctcc agaaaactca
     3721 cctggttttc tatttaaatg gtacttagaa agaccacaac tttctacaac atttaatata
     3781 ttatctttta tttcattctt tggcactaaa ttatgttcta taactgcctc ggatattatt
     3841 tgttccacat ttaatcttgg atttaatgaa ctatatgggt cttgaaatat catttgcatt
     3901 ttgggtctta actttctaag ttcttccttc tctaacttat atatatctac accattaaat
     3961 tcaactttac cttcagtttt ttcaattaat ctaagaatgg ttcttcctgc agttgatttt
     4021 ccacatcccg attctccaac tatagccaaa gtttctcctt tttttaaatc aaaagaaatt
     4081 ccatctactg cctttacaga tttaattttc ttatcaaata atccagattt aacttcaaag
     4141 tactttttaa gaccttctac ttttaataaa ctctcttctt ttttcaatta tatctcctcc
     4201 tttttatata accaacatgc aatactatga ccactattca attctattaa aggaggtatc
     4261 tgagttctac atttatcaca tactttttca catctatcac taaaataaca tgaatctggc
     4321 attccaattg gatttggtac ttgtcctgga atcgaataca atcttttatc tttgtttttt
     4381 ccaagagcag gctttgattt tagaagtcca atagtataag gatgttttgg atttttaaat
     4441 aattcaacaa cctctgccac ttctataatt ttgccagcat acatcactaa tactttgtca
     4501 cacatttctg caactactcc caaatcatgt gtaatcatca ttatagatgt gtttaatttt
     4561 tcttttatat ttttcattat atctaaaatc tgagcttgaa tagtaacatc taaagctgtt
     4621 gttggttcat cagctataag taattttgga ttacatgata aagccatagc tatcattatt
     4681 cgttgtctca taccacctga taattcatgt ggatatgaat tatatatttc ttcagctctt
     4741 ggtatttcta ccagttttat catatcgatt acctttttct tagcttcttc ctttgataaa
     4801 tcttgatgta tcataatacc ttcaatgatt tgacttccaa cagtaaacgc tgggtttaaa
     4861 gatgtcattg gttcttgaaa aatcattgat atctcattcc ctcttattcc cataagttca
     4921 tcttcattca tctttaaaat gtctttgcct cgaaagatta tttctccacc ttctattttt
     4981 gatgttggaa gtaacttcat caaagacatg gctgttacag acttaccaca tcctgattct
     5041 ccaactattc caatagtttc accttcattt atattaaaac ttacttcatt tacactcttt
     5101 acaatccctt tatctgtatg aaaataaact tttaaatctt taacctcaat tagtgcttta
     5161 ctcattattt tctcctctca gcaaaattac ttaagataac atacaccttt tgattgtatt
     5221 ttataaaaat ttaaattaca acattttctt acattttatt atttttttct attaattata
     5281 ataatataat agttaatttc tatatatatt gatttaataa tgattactat tatacaataa
     5341 aaaatatgtg ttaaatatta ttttatggat gttttataag aatatgaaat ttaaaaaatt
     5401 ggcatcatta atcttagttt catcattaat gcttacattt acagcctgtg cttccaatga
     5461 taaagacaag tctagtggaa gcaaatctgg tggaactgtt gtaataccta tggcaagtga
     5521 cccagatata atcaatggag cttttgcaaa cgttaaagaa gactctcttg catcaaatat
     5581 agtttatgct cctctttata cttatgaaaa aggaaattta gtaaattatt tagcagaaaa
     5641 agtagatttt aaagatagta aggaattaac tataaaatta aaatccaatt taaaatggca
     5701 tgatggaaag ccaataactg ctgaagatgt cttatttaca tttaatacag ttttagacga
     5761 aaaacaaaat tcaccatcaa gacaatactt attggtgggt gagaaaccag ttaaagtaga
     5821 aaaaattgat gatttaacag ttaagattac tttaccaact gcaagtgaat ctttcttata
     5881 tggtatatct aaaatttctc caataccaaa acatgtgttt gaaggagaat caaatatagc
     5941 taagtcagaa aaaaataata atccagttgg ttctggtgca ttcaagttta aggaatggaa
     6001 aaaaggtgaa agcatagtat ttgaaaagaa tgcagattat tttggtggag aacctaaggc
     6061 agattctata gcgctaaaaa taattcctaa tgaagcatct caagaagcag ctttaaataa
     6121 tggcgaaatt tcacttatga agacatctgc tgaaggatat gaaaaggcaa aatcaaactc
     6181 aaacttacaa acttatactt atagtgaaga aagattaaac tacattgtat ttaaccaaaa
     6241 tatatcaaac atggctaaca aagaagtaag acaagcctta tcatatgcct taaatagaaa
     6301 tgaaatgata gaatcagcat atggaaaaga aggttctgta cctgctaaat ctatattagt
     6361 tccagaagca gacttctata cagaagaagg tgttgaagga tatgaccaag atacaaataa
     6421 ggctaaagac ttattggata aaagtggtgt taaaattgat aaattaaaaa taggttataa
     6481 cacaggtaga tttgggcata aaaactatgc cttagtagct caacaagagt tgaaaaaaat
     6541 tggtatagaa gctgaaatag ttccttatga aagtaaggca ttcttcaata tactatttag
     6601 taatagcact gaatgtgata tgtatgtaaa tggttatgct tggggattag aaccaaaccc
     6661 atatagaggt atgtttgaaa ctggtcaata ttgcaaccaa actaaatatt ctaatgctga
     6721 aatagatgct ttatgggaaa aaggatttac tgagttaaac aaggaaaaac gtgaagaaat
     6781 atataagcaa atacaacagg atatatctaa ggatgcgcct atttacacta tagattatga
     6841 acaaaattta atggcagctc aaaaaaatct aaaaggaata aaagatgcta aaccatctcc
     6901 tgcaatatta tttgaagatt ggtctaaact gtatgtagaa taattgatgt aaaattttat
     6961 attattaatt agaaaaggag atgtctaaaa ataatattgt tttattattt taaaacgaga
     7021 catctctttt aattaggaga atttacttat gttaaaatta atattgaata gagtaaaagt
     7081 tttaatacct atgttaatct taatatctat attatcattt ggacttttag aattagctcc
     7141 tggtgaccca gcagatgcat atataaatcc tttaatgtct tcacaggata ttgaaaatat
     7201 aagggttaat atggggcttg ataaaccagt atatataaga tatttaaact ggttgaaaaa
     7261 cactttaagt ggaaacttag gtatttctta tataaaccat atgcccgtta cagaacaaat
     7321 tatggaaaaa atgggaaata catttattct tatgggtact tctttgatat tttcaatatt
     7381 ggtagctata cctcttggaa tatttttagc tgtaaataaa aattcaataa ctagtaaagt
     7441 atcatctgtt tttaattata taggtgtttc tataccttct ttttggatag gtatgatact
     7501 catttcaatt ttttcagtta aacttaacat tttcccatca ggaggtatgc atactattgg
     7561 aaatgatagt attggagact tagtaaaaca tttagttttg cctgtaataa ctttaggtct
     7621 atataatact gctattttta caaattatgt agaagctagt gtaaatgaac aattaaaaaa
     7681 acaatatgtt attacagcaa gagcaaaagg attatcagaa aaagtcatac tatttaaaca
     7741 tgtcttgaaa aattcactga catcattggt tacaatccta ggcatgagta tacaaaaatt
     7801 agtcactggt gcatttgtta cagaagtagt tttctcttgg ccaggtatgg gaagattaat
     7861 gattgattca attttttcaa gagattatac tgtaattatg gcaataacta tgttatcagc
     7921 actattttta attttaggta atttagttgc tgatatacta tacttattaa ttgaccctaa
     7981 aataaaatct agcaaaggag gattctagtt gtgtttacaa gtatcttaaa aaaattaaaa
     8041 aaagataaat cctttgcttt tgcatgtatt actcttagca taataatcat attaattata
     8101 gcttctttta tattaaatat agactctaat gcaattgata ctacttctaa acttcaagaa
     8161 cctagcttaa agcatatctt tggtacagac gaacttggta gagattactt ctctagagct
     8221 ctttatggtg gaaggatttc acttagtgtt ggtattttat caatgcttat ttctgtagta
     8281 tttggtactg ctgtaggtgt tattagtgga tatattggag gtaagctaga ccaattcttg
     8341 atgagattaa tagatatgct tatgagtata ccaagtttct tagttttgat tgtattaaat
     8401 acttatctaa cacctaaagt ttccactatt atactaatta ttggattctt aagttggatg
     8461 gaagtttcaa gaattgtacg tggcgaaaca ttaaaaataa aggaaaatga atactgtcta
     8521 gctgctaaag ctactggtat caagactaga aatatactat taagacatat agttttaaac
     8581 ttaaaagaga ctattgtagt tgctggttcg ttaaatattg ctaatgctat cttaactgaa
     8641 tctgctctta gttttttagg tcttggaatc caattgcctc ttgcatcatg gggtaatatg
     8701 cttcaagatg ctcaaagaca tatttttgac aagttatatt tagctgtatt tccaggatta
     8761 cttatatttt taacagtcat gagtttgact ataataagca aaaaattttc tagtaatact
     8821 taacaaattt tatatagaaa ataaaaacag tgattatcta aagatataca ctgtttttat
     8881 ttttcgttta aaaaaataca tattttgtat attataactt taaatactac tctaccatgt
     8941 tatgtaaaac ctattattgc tctcaaacat atagatgaaa aactaatagt ttattataat
     9001 aaaaattgat ttatttattt gttattttaa taacattttt aagagttctt tttactaaag
     9061 aaatctataa taaatttaaa tacatgttgg tctacatggc tataacgttc ttttttaggt
     9121 ctacataata taactttcca tgtaatagga tgattaaaag gcttttctat aattttgcta
     9181 ttttcaaaaa aatcatgaac tggtgatggt agaattgtta taaaatcaga atttgtggtt
     9241 gatagcaaca aaaaatccca tgaaccagac attatatatc tttttaactt aacattttca
     9301 tcagtaaatt tttgcattag cttatgatgt atcataaaag taggattaaa tattgcaagt
     9361 aactgctcat tcaaatcgtt ccaatctatc ttatccttct tagcaagtgg atgatttata
     9421 tttaaaaatg ctgtaagttc atcttcttga agcacatgtt cagttactac acttttatca
     9481 attttatgtg gatgtaacaa tactgcataa tctaattcct gtaagataag cattctattt
     9541 aagtcatatg cacccttctc tactatatca aattctatat ctggattatt tgcaactaac
     9601 tgtgcaacta catctgaaaa tgctattcca agtataagtg gaggtattcc tattctaact
     9661 tttcctttaa attgaactga atcttctctt aaatcttcca tcatattttt gtattcattt
     9721 atgatatgtt ctgcatttac ataaaatctt tctcctgctg gtgttaaccc atttaacctg
     9781 cctttatacc tttcaaataa atatacatct tcactttctt caaaattttt tataatttga
     9841 cttaatgctg gttgagatat gtgaagtgtc ttagatgcta ttgataaatt aaaaccagat
     9901 ttcactattt caacaaaata tttcaactgt tttatatcca ttttaaaccc ccttccccaa
     9961 aagtataatt tagattatat tttttcttct aatgatattt tataggaaaa tcaacataat
    10021 ttcattaata ttttaacaaa atatttttct aaatctacta taaattttta tcctataaat
    10081 ttttcttata cactatataa aaaattagac ttagacaaaa cgcttttttc gccgtaatct
    10141 ataattagaa acaggaaaac ctaaagcatt aggaataaaa gtaattgaaa tacttaaaaa
    10201 caagcttgta ttaaaaaagt actttattat agtgtttcct aatttcttta ttcagaaaat
    10261 ttttaattat cattcaagaa aggtaactat tagatatgaa agatgtagta atcgtatcag
    10321 cagtcagaac acctatagga agttttggag gtgtatttaa aaatacttca gctgttcaac
    10381 ttggaactat cactgttaaa gaagcgatat cacgtgtagg acttaattta tctgaaatag
    10441 atgaagtaat aattggaaat gttcttcaaa cagggcttgg acagaatgtt gctagacaaa
    10501 ttgctataaa tgcaggtata ccaaattctg ttccaagcta cacagtaaat aaattatgtg
    10561 gttcagggct taaaagtgtc cagctagctg cccaatcaat cacatctggt gaaaatgatg
    10621 tggtaattgc aggtggtact gaaaatatga gccaagctcc ttatatcgtt ccaactgctc
    10681 gctttggtag taaaatgggt gacataacca tggtagatag tatgctaaca gatggcctta
    10741 ttgatgcttt caatcagtat catatgggaa taactgcaga aaatattgca actaaatttg
    10801 aatttactag agaaatgcaa gataaattgg cattagaaag tcaaagtaag gctgagaatg
    10861 ctataaaaaa caaccgcttt aaagaagaaa tcgttccagt agatgtttta atacgtcgag
    10921 gaaaaataga aactattgat aaagatgaat atcctaaatt aggtatgaca tttgaaggtt
    10981 tagctaaact taaacctgca ttcaaaaaag atggtactgt aacagctgga aatgcctcag
    11041 gtattaatga tggtgcggct atgttaatac taatgagcca ataaaaagca gacgaactag
    11101 gtattagacc acttgctaaa ataaaatcct atgcttcagc tggtgttgaa cctgaagtca
    11161 tgggtactgg tccaatccct gcaactagaa aagcactgaa aaaagctgga cttagcatca
    11221 atgatataga cttaatagaa gcaaatgaag cgtttgctgc ccaagcatta gctgttaaaa
    11281 atgaacttca aatagattca tcaaagttaa atgttaatgg tggtgcaata gcattaggac
    11341 atccaatagg agcaagtgga gctagaatac ttgttacctt aatttatgaa atgcaaaaac
    11401 gtaaagttga aactggactt gcaacacttt gtattggtgg tggtcaagga atatcaatgg
    11461 tggtttcacg ataggaggaa atatgaataa aatagttagc attgatgaag ctctatctca
    11521 tgtaaaagat ggagatacaa taatggttgg tggattcatg gccaatggtt caccagaaaa
    11581 tttaattgat tatttatgta gtaaaaatat aaaagatttg acactaattt gcaatgatac
    11641 tggctttatt gacaaaggag ttggcaaaat ggttgtaaat aagcaattta aaaagataat
    11701 tgcttctcat gtaggattaa ataaagaaac tggaagacaa atgaataaca atgaaacaga
    11761 tgttgagtta gttcctcagg gaactcttgc tgaacaaata cgtgcagctg gttatggtct
    11821 aggaggtata ctcacaccga ctggtattgg tactcttgtt gaggagggaa agcaaaaaat
    11881 tgttgtcgat agcaaagaat accttcttga aaaaccaatt tatgcagatg ttgctcttct
    11941 atttgcatca aaggtcgata aagcaggtaa tctagtttat aaaggctcta tgaacaattt
    12001 taataattta atggcatctg ctgccaaaat tacaatagtt gaagcagatg aaatcgttga
    12061 aattggtgat attgacccaa atgaagttaa tacaccaggt atttttgtga attacatcat
    12121 tgaaggaggt aatttataat ggataaatta gaaatgcaag aatatattgc aaatagagta
    12181 tctaaagaat taaaagacgg agccgttgta aaccttggaa taggtctacc aaccaaagta
    12241 gcaaattata ttccggataa tgtaaacgtt atcttacaat ctgaaaatgg ctttttaggt
    12301 ttaggttcag ccgaagatgg aaattcaagt gatgaaacta ttgtaaatgc aggtggtcaa
    12361 ccagttacta tacttcctgg aggatgtttc tttgatagtg ctacttcttt tggaataatc
    12421 agaggaggtc atgttgatat aactgttctt ggcgcacttc aggttgacaa atatggtaat
    12481 atagcaaact atatgattcc aggtaagatg gttccaggta tgggtggagc tatggaccta
    12541 gtaactggag ctaaaaaagt tattgtagca atggaacata cttcaaaagg ttctgcaaaa
    12601 atattaaata attgtactct tccacttact gcaacaaatg ctgtagattt gatagttact
    12661 gaaatgggag ttatggaagt aacatcagat ggaattttac ttaaagaaat aaatccagct
    12721 ttcacattag atgatgttat ttctgctact gaggctcctt taatattgtc agattcatta
    12781 aatggaaaaa tatctgttgt ttaaaataat taaaactaga aaataaaaat ctcttagacg
    12841 tattacatat aaatttgtat tgcttaaaat attaatataa aaataaaata aaattggagg
    12901 aaatgaaaat ggttaaagat aaagttgtat tcgtaacagg agctgcaagt ggaataggta
    12961 aacaaatagc tgaatcgttc ttaaaaaatg gtgctaaagt aatgttctct gatataaatc
    13021 aagaagcttt aaataatacc ataacagagt tacaaaaaaa aggttatgat tgcatgagtg
    13081 taaagtgtga tgtaactaag gaagaagaaa ttaaccacgc tatagacaaa actgttgaaa
    13141 agtatggcag attagatata ttaataaaca atgctggact tcaacatgtt tctatgatag
    13201 aagatttccc aactgataaa tttgaattta tgataaaaat aatgctgaca gcacctttta
    13261 tagctactaa aaaagcattt cctataatga aaaaacaagg ctttggtcgt ataataaata
    13321 tggcctcaat caatggtgta ataggatttg ctggaaaggc tgcttataac tcagcaaaac
    13381 atggacttat tggactaaca aaagttgctg ctcttgaagc tgccgattct ggtataacag
    13441 ttaatgcttt atgccctgga tatgtagata ctccacttgt cagaggtcag ttcaatgacc
    13501 tttcaaaaac tcgtaatata ccattggaaa atgtcttaga ggaagtttta tacccacttg
    13561 taccacaaaa acgcctatta gatgttcaag aaattgccga ctatgctatg ttcttagcaa
    13621 gtgataatgc taagggtatt acaggacaag cttgcttact agatggagga tatactgcac
    13681 aatagttatt atatttaggg cttgaagaaa gtattcttca ggtccttcta ctaaggaggg
    13741 atatttatga gtattatcgc accaattggt ataattattg gtatcatagc tattatatat
    13801 ttttcagtta aagaaattca tataactata gctgctccaa tagcaacttt aatagttgtt
    13861 attttaaata aaatggatgt tgtaacatca atgctaggtg ctgacaaaaa caattatatg
    13921 ggtgcacttg gaaactatat aatgagttat tttgcaatat tcttattagg ctcaatactt
    13981 gctaaattta tggaagaaag tggagctact gtatcaatag ctgattttat tttaaataaa
    14041 tttggctcta atcatcctta tagagtttta gttgctatat ttatagtaag ttttatatta
    14101 acgtatggtg gtataagctt atttgtagtt atgtttgcgg tcattccact tgcaagaact
    14161 ctatttaaaa aacttgatat ttcttggaac cttattcaga taccactttg gcttggtatt
    14221 gcaacaatta ctatgactat aattcctgga acaccagcca ttcaaaatgt tatacctatt
    14281 caatatctaa atacgtctct tactgcagct actataccaa gtattgtagg tagtataggt
    14341 tgtgctacgt ttggacttgt ctatatgaaa tatgctctaa acaaaagtat tagaaaaggt
    14401 gaaacttatg caacttatac ttcagaatta gaagaagaat ctttagatag agaattacca
    14461 ggattttttg caagtatttt tcctctatta gcactcgtaa ttatagctat aactggaagt
    14521 acttttggtt cagaatttat gaaaaaaaat gtaatttaca tagctttaat agttgcaatt
    14581 cttttatctg cttttttatt ccataaattt ctatcatcta agatacagac attgagcata
    14641 ggtgctagtg gttctatagg tcctatattt gctacatctt ctgcagttgc atttggagca
    14701 gtaataatgg ctgcacctgg ttttgatgta tttagcaaac ttataatgtc aataccaggt
    14761 agtccactta taagtcttac tgttttgact tcaacaatgt cagcaataac agggtcctct
    14821 tctggtgcat taggaatagt aatgccaaac tttgcagatt attatttaag tactggtcta
    14881 aatccagaat taattcaccg tgttgctaca atagcttcaa atatactaac tatagttcca
    14941 caaagtggtg tttttcttac attcttaagc ttatctaagt taacacataa aactggattt
    15001 aaagaaactt tcattgtagt tgcagttggt tcactaatag cagaaattat tgtaattaca
    15061 ttaggattac taatgtatta atataaaaca aattaaaatt cataatatga aaggtggtgt
    15121 ctcaaagtga actttttact taatgagaca ctctttttat atgaaataac atatgcctat
    15181 catttaaaca ataaaaaagg ccttatctct attttgagac agaccctttt tatttcttaa
    15241 agatttaaat aattataatt tgttgctaat acgaaagcat ctactgaata aatttaacca
    15301 aataaaaaaa aatttatcat aactataaat ttacgttatg gaattattat aaaaatatta
    15361 ttttctctaa ctaagtaatt ccatactata caggctttta atacaaattg tcaaacacac
    15421 tgaaatatac ctatttattc catatatatc ttttttacat atctttacag taaaaaaagt
    15481 cttaatctta tgttagtata gattcacggg taacccccta cactaaaggt tacaaatcag
    15541 taacattttt agaattttta gattaagagg tgtttaatga tgagaagaaa tataccaaca
    15601 aaattaaaaa ctatattagc tttaggagtg ttaactttag caacagcttc aagtgctgtt
    15661 actgcaagtg cttcatcact tgaaaataat gtaaatataa gtaacaaaag tttagtatat
    15721 agtaatacta aatacaagtt ttctgcaaaa ccaaattgtt ctgtaaaacc agaacttaag
    15781 cctgataaag ataactcttg taaggataag aatcataatg acaagaattg caacaacact
    15841 aataaacctg aggacaacaa taattcagac tctactaata agcctggtaa caataaacct
    15901 gagtctaata aacctgaaga caacaataat tcaggctcta ctaataaacc tgataataat
    15961 aaacctgagt ctaataaacc tgaagacaac aataattcag gctctactga taaacctgaa
    16021 gacaacaata attcagactc tactaacaaa cctgatgata ataacaactc aggctctaca
    16081 agtgaaagtt tttcagctta ccaaaaagaa gttgttgact tagtaaatat agaaagagct
    16141 aaagctggat taaatcctct tactcttgat tcttctatat caaatgttgc tacaaaaaaa
    16201 tctcaagata tgattgataa caactatttt tcacacaatt ctccaactta tggttctcca
    16261 tttgacatgt tgaaaaaatt tggaataagc tataagacag caggtgaaaa tatagctatg
    16321 ggtcaaaaaa ctcctaaaga agttgtaaat gcatggatga attctgaagg tcacagaaaa
    16381 aatataatga atccaaattt cagtaaaata ggtgttggag tagcacaaaa aagtggtggt
    16441 tcaatttatt ggacacaaat ctttgtagga taaaataatc tttttccata catatcaaaa
    16501 gattatctct aagtgataaa aaacacaaat gagatactct tttgtatcta ttttaaaatt
    16561 atcaaatcca aatatacata ctataaatat tatttgttct cttaatttta gtgtgtagag
    16621 caatatataa aaagtattgg caagatgaaa atggaaaact taaaaagata tttaataggt
    16681 aaaaacagga tcttaatatc ttcataaaaa aaggctgtct caaaatagag ataaggcctt
    16741 tttcattgtt gaaatgatta aaatatattt gatttcatac aaaaagagtg cctcatgaac
    16801 taaaaagctc actttgagac actctctttt atcaattcta tataagcatt ataatacttt
    16861 aatattattc agctaatttt ttgtatcctt catatctttc tttagcatca gcttcagctt
    16921 tagcaaataa tccatcagct tcctctggga attgtttagc tatagcagaa tatcttactt
    16981 gtgcaagtat gaaatctttg aagcttgcag ttggttcttt tgaatctaaa gtgaatgggt
    17041 ttttaccttc agcttttact tctggattga atctgtataa atgccagtat ccgcaagcaa
    17101 cagcttgtgc ttcgttagct acagttcttc ccataccttc ttttaatcca tggtttatac
    17161 atggagcata agctattatt aaagatggcc catcatgttt ttcagcttct ataacggctt
    17221 tgataaattg gtttttatca gcacccatag caacttgagc tacatatacg tttccatatg
    17281 tcatagccat cattcctaaa tctttctttc tagatttttt acctgaagca gcaaacttag
    17341 ccatagcagc agctggagta gctttagaag cttgacctcc tgtatttgaa tatacttctg
    17401 tatcaaatac aagtacgttt acatcttcac cagatgcaag aacatggtct aatccaccgt
    17461 aaccgatatc atatgcccaa ccgtctccac ctagtatcca ttgagatctc ttaactagat
    17521 aatcttttct atcttttata gcttttacta actctttagc tttttcatct gtacaatcac
    17581 aagctggttt ttctaataat tcaacaacct tagcacttgc tactttagaa gcttctccat
    17641 catttctaga atctaaccac tctgtaaata ccgctttagc atcttcacag atatccatag
    17701 aaactaactc tgtcatagca tctactattt tatttcttat ttgcttaaca gctgtgtaca
    17761 taccaaatcc atattcagca ttatcctcga ataaagagtt tgcccaagat ggacctttac
    17821 cttcatggtt acaagtataa ggaattgatg gtgcagaagc tccccatata gaagaacatc
    17881 cagtagcatt agctatcatc attctatctc catataattg tgtaactact ttaatgtatg
    17941 cagtttctcc acatccagca catgctcctg aaaactccat taatggttgt ctaaattgac
    18001 ttcctttaac agtgtttgca ggcataacat caccttttgg agctacctca tcaggattta
    18061 ctgcaaatgc ccaattatct aactctgtat caagttgagt atctattggc ttcataacta
    18121 aagctttttc tttagctgga catatgtcag cacagtttcc acatcctgta cagtcaagtg
    18181 gactaagttg cattctatat tttaatcctt ctaatccttt acctaatgct tttttagctt
    18241 caaatccttc tggagcatta gctaattctt cttcagtaac taatactggt cttatacatg
    18301 aatgaggaca tataaaagaa cattggttac attgaataca gttatctatt atccattcag
    18361 gaacatcaac agctatacca cgtttttcat aagctgcagt accacaaggg aatgttccat
    18421 cttctatacc attgaatgta cttactggta agttgtttcc ttcttgtgca gtcattggtc
    18481 ttagtatatt ttttataaat tctggttctt taacttctcc tttatctgca tcaacagcat
    18541 tttcccaaga agctggaact tcaactttaa ctaaagcatt tattcctgca tcaacagcag
    18601 catagttcat gttaactatt ttttcacctt ttttaccata agctttaact atagaatctt
    18661 ttaagtattt aacagcatct tcttctggta ttatttcagc taatttaaag aatgcagatt
    18721 gcattatcat gtttattctg tttccaagac ctatttcttg tcctagttta acagcgttaa
    18781 ctgtatagaa ttttatatca tttttagcta tatattgttt catatgagct ggtaaatgtt
    18841 cttcaagtcc tgcttggtcc catatagtgt taagtacaaa tgtaccacct tttttaagac
    18901 cttttaataa atcatattga taaacatatg attgtttatg acaggcaata taatcagctt
    18961 catctattaa ataagtagct cttattggag tatcaccaaa tcttaagtga gacatagtta
    19021 taccaccaga ttttttagag tcataatcaa agtaagcttg agcatatttt tcagtatggt
    19081 caccgattat tttgatagct tgtttattag ctccaacagt accgtcagaa cctaatcccc
    19141 agaacttaca tcttatagtt cctttagaag ctatttttat tggctcagat ggagttaaag
    19201 aagtattagt tacatcatca actataccta ctgtaaatcc atttttaggt tgttctgaaa
    19261 ctaagttatc aaaaacagtt tttatatctg aaggagtagt atcttttgaa cctaatccgt
    19321 atcttccacc tattatcata ggagcatttt cttttccata gaatacatca cgtacatcta
    19381 agtataatgg ttcaccagtt gaacctggct ctttagttct atcaagtaca catattcttt
    19441 caacagtaga tggcatagca tctaagaaat gtttcattga gaatggtcta tataaatgaa
    19501 ctttaactaa accatatttt tctcccttag catttaagta atctatagtt tcttctattg
    19561 tttctgttac tgaacccata gctattaata cgtatttagc atcttctgct ccatagtaat
    19621 caaataaact atgttttcta cctgttaaat tactcatttt ttccatgtat ttttcaacta
    19681 tacctactat attttgatag tatttattag aagcttctct agtttggaaa tatatatctg
    19741 ggttttgagc agtaccacga gcaactggat gatttggaga taaagcatta tctctaaatg
    19801 cttgaactgc ttcaaagttt aataaacttg cataatcttc attttctagt aattcaactt
    19861 tttggatttc atgtgaagtt ctgaatccat caaagaaatg tataaatggt aatctacctt
    19921 ctattgcagc taaatgtgca acaggtgcta tatcagctac ttcttgaact gaaccagaag
    19981 ctaataatac acatccagtt tgtctagcag ccataacgtc ttggtgatcc ccaaatattg
    20041 ataaagcttg agatgctaaa gcacgagcac ttacatggaa tactcctggt aacaactctc
    20101 ctgcaacttt atacatgtta ggtatcatta ataataaacc ttgagatgct gtaaaagtag
    20161 aagttaaagc tcctccttgt aaagaaccgt ggaatgttcc tgatgcacct gcttctgact
    20221 gcatttcaac aacattaact gtttgtccaa atatattttt tcttccttgt gatgcccact
    20281 catcaactac ttcagccata gttgaagatg gtgtgattgg atatatagct gctacatctg
    20341 taaacgcata ggcaacatga gctgcagctg tatttccatc aagtgttttc ataaacttag
    20401 ccataatatg tatcctcctt gatagctaca attgtacttg tttataacaa gtctattttt
    20461 ttatatttac atataattta cacaacaaaa ctataaacac acatatagtc gcgcaaatac
    20521 tctatcttct aatata
//
LOCUS       DACULQ010000059        20088 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.059, whole genome shotgun sequence.
ACCESSION   DACULQ010000059 DACULQ010000000
VERSION     DACULQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.059"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            218..673
                     /locus_tag="JA154_08590"
     CDS             218..673
                     /locus_tag="JA154_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995923.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(880..1140)
                     /locus_tag="JA154_08595"
     CDS             complement(880..1140)
                     /locus_tag="JA154_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995924.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(1403..1756)
                     /locus_tag="JA154_08600"
     CDS             complement(1403..1756)
                     /locus_tag="JA154_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995925.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANVEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(1773..7094)
                     /locus_tag="JA154_08605"
     CDS             complement(1773..7094)
                     /locus_tag="JA154_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995926.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDVFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNALISQYT
                     EISLSNVTDAYSSYNATFIVVDEKKVYATGINTNYLLGFSTSDGSNVNLGLLSDWYYI
                     NISGSSYSRVSCTNNITKINNIIIYEYVTVFCTNIGSFLTGYHGTSWTKPTDSSYRVQ
                     YQGISYAGYLDSYIYNYYPTRCTQSSSSTTFAYLYNGESSSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNIFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYLCNDVSIIVKNDNTCYVCGLVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(7111..8094)
                     /locus_tag="JA154_08610"
     CDS             complement(7111..8094)
                     /locus_tag="JA154_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAT7995927.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(8095..8793)
                     /locus_tag="JA154_08615"
     CDS             complement(8095..8793)
                     /locus_tag="JA154_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995928.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(8790..9842)
                     /locus_tag="JA154_08620"
     CDS             complement(8790..9842)
                     /locus_tag="JA154_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein J"
                     /protein_id="HAT7995929.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(9835..10263)
                     /locus_tag="JA154_08625"
     CDS             complement(9835..10263)
                     /locus_tag="JA154_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HAT7995930.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(10263..10589)
                     /locus_tag="JA154_08630"
     CDS             complement(10263..10589)
                     /locus_tag="JA154_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995931.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(10605..12134)
                     /locus_tag="JA154_08635"
     CDS             complement(10605..12134)
                     /locus_tag="JA154_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HAT7995932.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGIIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(12416..12838)
                     /locus_tag="JA154_08640"
     CDS             complement(12416..12838)
                     /locus_tag="JA154_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995933.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(12838..15291)
                     /locus_tag="JA154_08645"
     CDS             complement(12838..15291)
                     /locus_tag="JA154_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995934.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(15291..15458)
                     /locus_tag="JA154_08650"
     CDS             complement(15291..15458)
                     /locus_tag="JA154_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995935.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(15485..15931)
                     /locus_tag="JA154_08655"
     CDS             complement(15485..15931)
                     /locus_tag="JA154_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAT7995936.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(16045..16473)
                     /locus_tag="JA154_08660"
     CDS             complement(16045..16473)
                     /locus_tag="JA154_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAT7995937.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(16487..17551)
                     /locus_tag="JA154_08665"
     CDS             complement(16487..17551)
                     /locus_tag="JA154_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HAT7995938.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(17556..17999)
                     /locus_tag="JA154_08670"
     CDS             complement(17556..17999)
                     /locus_tag="JA154_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995939.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMKEINLNLGE"
     gene            complement(18248..18688)
                     /locus_tag="JA154_08675"
     CDS             complement(18248..18688)
                     /locus_tag="JA154_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995940.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(18793..18990)
                     /locus_tag="JA154_08680"
     CDS             complement(18793..18990)
                     /locus_tag="JA154_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7995941.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            19159..19479
                     /locus_tag="JA154_08685"
     CDS             19159..19479
                     /locus_tag="JA154_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7995942.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            19561..20067
                     /locus_tag="JA154_08690"
     CDS             19561..20067
                     /locus_tag="JA154_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995943.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
BASE COUNT         6242 a         3546 c         2106 g         8194 t
ORIGIN      
        1 gcttttgata cgattttaca tcatttcttt gttctaatat ttttatttca tttattttct
       61 tttttataat tttatgataa aataattagt atattttgct ttacttgaat gtaattattt
      121 atacaaaagt caaacataat ataaatgtag taaattttta gtttcaagta atatctcgtt
      181 attataacta aatatttatt aaaattagga ggataaaatg ggcttaagag acaaattcgc
      241 gcaatcattt gctagatcaa aaactatgag tggccctgag aaaaaggcta atgaaataat
      301 gggtaaattg cttcttaaga aagctatatt acctatcgtt ttaatgttcg tgataataat
      361 cgctggagct atgcttaaaa tcaattcttg ggttacactt ggcataaatt tagttattgc
      421 tgttggtgct ttcttctaca taagaaattc tagtaaaaag tatcagaact ttaaacctta
      481 tgttggcaat ttgattagtt tagagaaaaa aggtaaaaaa gagtacgttg ctattataaa
      541 acaaggtaag ttaccagtta agttacaaat agcttatggt ggtgaagacc ttgaacatgt
      601 taagaaaaat caaatggttc aaataagtta taatccagat gctaaaatag ctattttggt
      661 taatagacaa taatttaata aaaaaaagga gttgtcttaa aattgatttt ttctaatcaa
      721 atttaagctc aactcctttt ttaattttta tatgttattt ttattacttt ttttaattaa
      781 tattttaaat attaaatcct tctcaataaa atagtgaact atctacaata ctactttatc
      841 ttttcctaac aatatataat ctggtgctac tctttcatgt tatacaccat tttgtaacat
      901 accttttata tcagtaacat cttccttaat ttcctctaca tctgttttca ttatacccat
      961 ttgaacaagt atatttttgt ttatttcttg ttgttgtgta gataattcta taaaattttc
     1021 tacagttttt ttatacatgt ctctatcttc ttttttctcc tgcatagtat ttttaaataa
     1081 tagagcacat agtataccta ttgcccctag actactcaat tcggttatta atttatccat
     1141 tatagcctcc tattgtctaa attttttata cttatagctt taatttttgt tgtattctta
     1201 catcatgcaa tattatattt tcaggattat caatttaatc ttctgtactt taaattgtag
     1261 gtgtaattta tagattgaat aaataataaa tatatataca agttctaatg taacaaaaaa
     1321 taagtacaag gcttacttat taaagttaag accttgtact ttacataaaa aaaataacta
     1381 atacatagtt attctaaatt tactattcaa ctggtatttc taattcttta catctaattc
     1441 taacttgctc tctaattact gctggtactt cttctattgt ttttttgcct cttaaaatta
     1501 aagctacata tacttctacc attccactct cacctccaga ttttcttaca ctaaaagtgc
     1561 ttatattact tgcttcattg acagaacttt gatttaacaa catctcatat atttctacat
     1621 ttgctataaa attatttgtt atatcatcat cttgctttac ttgacctata cttatgtctg
     1681 aaatgtcttt atactgcatt tctacttttc taccttgttt gatttgttct attttatttt
     1741 tatcgaattt aaacaatttt cacacctcca ttttaagctt gtggctcgtt gttttcagca
     1801 tcatatctaa aaatcataga tggacgtttt atagctgtcc atttatctcc tttttctact
     1861 atgattttag gctgagcact ttttatgttg gcttcttcta attcatagat ttcttggtaa
     1921 cttgagtctg attttttgat ttcaactagt ttcatgtctt tgaataagtc agatttattt
     1981 atttttactt gaactttagt tttatttatt aattgatata taggattgtt ttccatcata
     2041 tcatccttta atccctcatt taatgttact attaagtcag tgttattttc agtaatgttt
     2101 atgattgaat atactttatt gtttatcatt aaaatagaat ccttttttac tttaagccct
     2161 gtttctgatt taaatataaa catacttgaa tataaatcgc ctgtaggact actggcttta
     2221 atcagtatct tattttctcc taatactatt ttattttgtg gtatctcaaa tataatgcta
     2281 tttatttcca aattagtctt agtagatatt aattcatcat ttataaacat ttcaagtttt
     2341 tctataacat tattagggtc ttgtactaca actctcactt gagatattga tttttcaact
     2401 atttctatag atgctttact tgtatctact tttgttatca tgtatattgt atcatctgaa
     2461 agctctatat ctaaaatatc atttagatgt tcaggaattg gtattttagt aaattcatat
     2521 aaatcagtaa ttgatataga tattttatac atagatttat ttttaccagt tatccataga
     2581 ctattatcat ttttaattac tagtatgtgg tcatcattat gtgacttaat ttctttaaca
     2641 ttagttaata ctttagtaaa agttcttatt atcttatctt tttcactttc tcctagtcca
     2701 aataatccat ttgtattgta cccttgagcg tacaactctc cctcattagt taaaacaagt
     2761 gtcattgagt tatatatact tatatcttta acagaaggta atccagtaac cttttgtaac
     2821 tctcttacat cattattgtg gccaactccc aattggtact cagtattctt tccatatgac
     2881 catagagtat tatctgtctt aataatatag gagtagttat ctccacaagc aacttttttg
     2941 acatctgata tatttacttc agtaagagtt aataattcta tatcttcaag ggtttgatta
     3001 atttctttat atgaattact tcctattcca tataacttgc catccttagc aacagccaat
     3061 gaatgtgttt ttgatatatc aatatcagat acctcaaatg gcaatattat tgaagtatac
     3121 ttaaaattat ctttaaatat gtttttataa tttatatact tattatttgt ttttatatac
     3181 ttatcattaa acattactag cttttcattt ttcttattaa aaactatttt atctgatgtg
     3241 ttatctgttt taaaatatga atgtagggaa ccttcaagag cttcattttt atcattatca
     3301 tatctatggt gaatacctaa atgattatat ctattatctc ctgttgaaga ataaaatttt
     3361 ccatatatat ctttaataaa tatagtattg taactagaaa atacagtatc aacatctttc
     3421 tttatagata ctttataata tggaactgac atatccctat aagattcatc agatatacca
     3481 taagtatctc tacctgatga tatcaaattg ttattattat ctatataaag tgtattatta
     3541 gctgttacaa aaacatcttt aacatcttcc acactatttg tattattatt atttgctata
     3601 cttccatatc cactaagacc tccaacaaat agtttattgt catttgttaa aatggctgaa
     3661 gtatatccat taactactat tttttttgca tttataatat tattaattac tcgtatcccc
     3721 tttatatcat tagtctctgt tgaaaagaat accctctctt ttttccctgt agtataaatc
     3781 atattgtcat ttgttataaa aaatgtagct tcacttcctg ctgtcacaac agattttaca
     3841 ttctcaatat ttatttttgt aaatgtactt atacttcctt cagtaaaccc aaaatagcca
     3901 tttacaagtc cacatacata gcatgtatta tcatttttaa caatgattga tacatcatta
     3961 catagatata catctctaac accatttata ggtatacgtg taaatatatt cctacttgta
     4021 gtatcaccca atcctaactg tccttttcca ttattacctg ctccatataa aagaccatca
     4081 ttagttaata atagagatgt gtttccatat acagctacat attttacatt ttcaacattt
     4141 attttagtaa atatatttct atttacatta tctcctattc ctaactgacc agaattattt
     4201 aaaccaacag cataacaggt attatcattt tttatcgcaa atgcatgttt actattgtca
     4261 gtaaatctat ttacatttat agattttata ttatcaatat ttattttagt aaataccttt
     4321 ctatttatct catctcccac tcctaattga cctacatcat ttaaaccaac actgtaaagc
     4381 atattattat tagttaataa atatgtagac tttgaatggc tatatatttc ttgtatatta
     4441 gaagatattt cactactgct attcttttta ttgtaattta tcttattatt taaatttaaa
     4501 ctactattaa ctgatatacc atgcgtaccc ttagagttat atccacatat aaataagctc
     4561 ttatcagtta atattgcaaa tctagtttta tctctactta aatcataagg aggtataaat
     4621 atatttttta tatttttcat taccttttta taattagtgg cattatatcc atcttgtgta
     4681 tctgaaacat ctagttcttt tgcactttca gatatattcc caaaaccata tagcgtgtta
     4741 gaagataaat acaatagagc tttatataaa aatatggctt tatcagaggt aataggacct
     4801 gaatttatat tagatgctgc aatattatta gatgattgat tgttaagata attccttcca
     4861 tattgatgta tataagatga acctccagaa ataagtaaat tatctggatt gacatttttt
     4921 aaattacttg acgattcccc attatataaa taagcaaaag ttgtagaaga tgatgattgt
     4981 gtacatcttg taggataata attatatata taagaatcaa gatatcctgc atatgaaatt
     5041 ccctgatatt gaactctata gcttgaatca gttggttttg tccatgaagt accatggtat
     5101 ccagttagaa aagatccaat gtttgtacaa aatacagtta catactcata tatgataata
     5161 ttattaattt tagtaatatt attcgtgcat gaaactctac tataacttga ccctgatata
     5221 tttatataat accaatcact taataaacct agatttacat tagatccatc agaagtacta
     5281 aaacctaaca ggtaatttgt atttatacca gttgcatata cctttttttc atcaactaca
     5341 ataaatgttg cattatatga actataagca tcagttacat tactaaggct aatctcagta
     5401 tattgactta ttaatgcatt actaagtcca ttaacataac ttgaactctt tccagaagtg
     5461 tatatttgct tattattagt tacaattaat gaaaaccagt ttgataaatc agtatttata
     5521 acaacttttt ttacattatc tatagatatt ttcttaaata catctgaatt agaaggaaga
     5581 acacctgcta ttgagttact atttggtcca gaataataac aagtatcatc atttttaatg
     5641 tagaatattc ctccagaaat attttttgat gctactatag actttacatt agagatactt
     5701 aattttgtca actcatttaa ttgacttggt aagtcagaac ccattccttt gtatctatta
     5761 tttcctagac catatacatc tccattccct aaaataacca gagtacaata aggactaaaa
     5821 acgacatctc ttacagaatg tatatctagt ggaagttcaa tatcctttat ttccatttct
     5881 tcttttaata aagttaattt atcctcagat ttaattaaat ttatataatt actataacta
     5941 tatttttcac tatccatttt cttaaattct acaatgtgca tatcatcaaa aaacgaacct
     6001 aattgtccat aagtattatc tcctgtacaa tatgaatcat tatttgaatc aattagtatg
     6061 ctatgtgata aaccaatgaa aatattctta gctacaatat tttttaaatc tataaatgaa
     6121 ttatctatat tctcagagta atttgctata cctaactgat atatatcatt atttccagca
     6181 acctttaaag tacctttgtt atctataagc atagtgttac catgaccaca tgcaatagtt
     6241 tttatattta cttcttctat cttagtaaat agtgatgatt gaagtgcatt tccaaaacat
     6301 ttttcctttc cacaaccata tatatcatta ttatacgtca ttaacacagt gtgttcagaa
     6361 ccacaagcta tagtcttaac atcattaata gaaactaaac taaataaatc tctattattc
     6421 ttatctccta aacctagttg accttgatta ttaaaaccag tagaatagca tttattgtca
     6481 ttctttatta taaaagctga tttattatat gaaaatacag cctttacatt gtcaacatct
     6541 acttttgtaa atgtatttac atttgcagat gctccattag ttcctagacc taattgacca
     6601 tacaaattat ttccagcaac atataaagtt ccatcattgt ataaaagata tgttgattga
     6661 agtccacatg caatatcctt tacgcctttt atttctggaa ccttctcaaa tgtagaaact
     6721 gttttcccta ctttttctaa tcccaatccc atttgaccat ctgtatttat acctgcgaca
     6781 agtaattctc catctgaagt aagtataact gcatgagatt ctccacatgc aactttttta
     6841 atattggtag atatatttgg tattttcgta aataattttc ttgttgtatt gtcttctccc
     6901 aatccaagtt gaaagttact attatctcca catccataca agatattatt attttttaga
     6961 ccaaatgtat agttacaaca tccagtaaaa aaattaataa agtcataatt atcataccct
     7021 tcatcaaatt catcaactac catattttta tcgccaaaga aattacctct ttgaagtagt
     7081 ttagttcttt tcattataaa aacctcctaa ttatcttatt tcatacgaat atttatttaa
     7141 agttatatct gtctgaccaa ttgaatatgc cctattagat tttgtttcaa caaagttatt
     7201 atccatttca tgtatatatc taactctatt tgcattaaat ggtagcacca cattcttaaa
     7261 tgtaacacta ccaagttctc cagttataca ctttctattt aaatctaact taaggccttc
     7321 tatagctatt atgtttttag cattagttaa ggtatcatac caatatccag cttcatctac
     7381 atctatttct gactctaatt ctaagtatat taagatatct aatacttttt cagataattt
     7441 attgtaacta ttttttaata cagatagttc agtttgtatt tgacctccta ttacatcatc
     7501 aatagatata tttttaattt tttctactac ctcatttata gctcctgata agtttttagc
     7561 ttctgtagat aagagttctt cgccaatttt tacttttatt tcttttatat ttttatcaaa
     7621 cttatcatct aaatcttgaa tatccttttg agttacaaaa accaacgtcg ggtctacttc
     7681 taatgtaaca tttgaagtat tggacaattg taaaatcatt tttacaacca aatctttaat
     7741 actgccatct tcagcacgtg gcttatacgt ctctggatac ttacctatag ctattatatt
     7801 attctcatta tccagaacag cagcttctct tatcataaat ccaccaactg ttcctggtaa
     7861 aatagtctct ataactatcc aattaggatt tttttcatca attcttagag aatttatatt
     7921 tccttcccaa actacattct ttaaagcagt ttgttcctct gttggctcat aatattctcc
     7981 attactatct ccaacttgaa acttggttaa tccaacaagc tctcctagtg cagttgcatt
     8041 tgctattttt tctctaccaa ttttggttaa tattatataa aatttttctt ccaattatac
     8101 cacctccgat ttaggataaa ctactacatt ttctacacct gtatcatttg caatagctat
     8161 attaatctta caattatttt caatgcattt aatgcttaaa ggataaacct ttatttcttc
     8221 tctacttatc attccagctc cacagtttac aacagcacta cattttataa ttggttcaaa
     8281 gtatggatga acttttacct cttcactaca taccatacct ccaccacaat agaccatact
     8341 tttatctagt attattggtt ctaaatagtg taataagtga gcaggtttta ctctttcaat
     8401 catatcgcta caatctaaaa gagttttata gtcacagtta tttgctataa aactcaatac
     8461 gaatgtaaat tcatcactat aaacttttat atctgtttct gattttgtat atgcctcaca
     8521 tatacttttt ataacttcaa tagtactagt acctctgctt ctcattttag cttttatatt
     8581 gctacgtctt gtgtcaaaat caagttcttt tttttcaatg cagagtatgt cttcccacat
     8641 atctaatccc catgttgcag tgtcaacaaa taattggtta aaggtatcat caatatcttc
     8701 tttaagaata tttaattctg tatcgtatgc accttgtatc tcctctacaa tgtaatttct
     8761 atcaaatgat ggtagtttat caattagctt cattgatttt ccacctcact aaaattaaca
     8821 gttgttacac taggtatttt atcttcatta atagttatat tatctgtact tccatttata
     8881 agtaggttac ttaaatcgtg tacaccagta gtatttataa gtattcccat tacttttgta
     8941 tagattatct ctcctctaat atctctaaag tatgtattta tactttctag gaaagattct
     9001 tttacattgt ctaatgtata tccatcttct agtttcatta ctgcacttat acttatttct
     9061 attggtaatg gtgtcacaac tgttatagtt ggtccaatag gcttctcttc atctatatgt
     9121 tgctgacacc tttcaattgt ttctgtatca acagcttgat tatttttccc aaatatcaag
     9181 accttaactg ttcccggacc atcccatctt ggataaacct taacattata gactccatct
     9241 acttccaaag cccattcttc atagtgagct ttatttccac ttgtagcttg atttctttgg
     9301 attttataaa atctttcttt tagttcttca tctgtctcta tatctgtacc accttcaaaa
     9361 cttcttgtgt tagtaatctt tgttactcca gatatattat caactagttt aaattcacaa
     9421 tttgtaggta aattatattt cttaccaact tccagagctt gaactgggct attgtcacct
     9481 tcttcactac caatagttac atcttttatt actacaaata gtaaatctct atatgatatt
     9541 attgtgccat tatttattac agtacctttc tctccaataa attcaacttc tccatttgac
     9601 tctgtaccta actttctata tacaccaaat tcattgactc ttttatcaag aaactggtta
     9661 tatgtgtctt gtataaaagc cattttatgc atctttgaaa gttctagata tatcttcgaa
     9721 agttctagat tatttccaga taccatgttg tttagaaaag aaccttctcc tttataaata
     9781 tcaagattaa tattttcaag agttctattt tttattactt catatgtctg gtcactatac
     9841 atttatttcc acctccccat agatagtgga tacttttaca tttgcactca atatgtctct
     9901 gttaaagtta gcacttttta cgtttacttc taatatatat ggatttatta gaagggcctc
     9961 ttttatgaat ctactagctt cactttctgt aagacctttt gtatattttt gtcctattag
    10021 ttctgaaagc tctgtcccat attctaaaga gtatatctca tgttcatatc tatttgtctt
    10081 tatacacttg tatatccaaa cttttaaggc ttcttttttt tcaattattt taaagtcacc
    10141 tttttctaaa ataggttcat ctttttcaaa atcccatgcc acttcacgaa agattggcaa
    10201 ttcttctgtt ttaggtaaga tataatcctc tgggacacct ataaaaggaa atattgtact
    10261 cattataagc tcaccaactt acttacaaca gcaaattttt cgcctattct aaacattatt
    10321 accatatccc cagattcaaa gttatctata aaaggatttt ttacttcatg ctggtgttct
    10381 tgatttgttt ctgtgtcaaa tgtttctagc tgtctatcaa tcatccaact atctatcaaa
    10441 atatcttctt tttctaatat gatgttattt gtctctattt ttaaatctgg taatttactt
    10501 ttaatttttc caataaaaaa agaaggttga ttatgatact ttccttcttc tcttattatt
    10561 cctataaatt catttattgg attagccatt atatcaccat ccttttataa tactctccta
    10621 gctgtgttaa aatctcttct actacttaac ttacttattt taactacatc acctttttgt
    10681 ggagcatgta aaaattctcc attacctata tataatccaa catggctgac tggattatga
    10741 aaaaatacta aatctcctgc ttgaagattt ttttgttcta ctttcttgcc ttttttagat
    10801 tgttgatttg acgttcttgg caaactaaca ttaacttttt taaaacaata gaccattaaa
    10861 ccagaacagt caaaactact tggtccattt ccaccccatt tataaggttt acctagatgt
    10921 ttttttgctt ctgatattac tattttagct ttatcagata cattaccaga ataaccacca
    10981 ataataattt ttccttttct tcttccaaat ttactagctt cttcaacatt tccaaataaa
    11041 atatctatat ggtatgttcc attcttttct atagttattg caggtcctac gtcatttact
    11101 ttatagactc catcacgacc agaaactcct gtaattacct gtatttcatc tccatagttt
    11161 aataaaggat gtttatttaa aaactctttt gtataataag tttgctcata tttaccaacc
    11221 ataggagcag cgcaagtttt tttagatggg tcaagttttt tctttctaca atctgtatct
    11281 ccaccttctt cttttctagg acagtaagct gtaaattctg ctgtaaactc ttttcctcct
    11341 gcataatctt ctccccctaa attactttct tccttaggtt catcctgtcc tgctgacttt
    11401 tcatccataa gattttgaaa attaagttca agctcaattt gatattctcc attttgccaa
    11461 gtatgtttgt ctgtatctat ataaaatagt cctacaagct ttgtataaga atctttaact
    11521 tttactcctc taccagttat acaacttaca tctccataac ctttaagaga acagcttttt
    11581 tctatcccat taaactcgct atcaatatct acatcttgat tttcttgttg ctgaattact
    11641 ttttgcatta ttacatttac ttccttaaaa atttcattat ctattttttc gctaatcttg
    11701 cttccatact ggtctaccac tattacttta ttttttacat tttccatgct ctccgaaaag
    11761 gtggtattta taatattaaa tccctcttca aacataacac ttaatgtaac agttcctttt
    11821 tcaataacat taaacttatc taaattggcc tctatcatat actttttctt tgtcttttta
    11881 cttgcttctg tatatgcact cattattgtg tcataaccat ttactcctat aaacatcttt
    11941 gtatacttga cattggtctt tgctattatt ccaactgaaa gcctattttc agcaaatact
    12001 tgctttgcta tgtcttcaac taacttatct ttaaaattgt atgacacttc actttgtgta
    12061 agtaaaaatc ccatatcttt agatacaaaa ctaatttcat tactgcttga atctttagac
    12121 ctattaatta tcattcctct aaagagttct ttctcatcta catagaaaca gactgtacta
    12181 gctattggta tatcgatttg ttggaaattt acatcaaatg atgattgtat tattgaaaac
    12241 tctagtgtcc ttgatggaga tttataatca cctgaccatg ataccttgtc tactatgtca
    12301 gttatgtcat atatacttcc gttttttatg tgtacccaaa tcttaataat aaaaaatcac
    12361 ctgccttttc atttaattct atcagcagtt atttataact tattatctat aatagttatt
    12421 gttttggagt tacttttggt atctttattc ttttatactc ttttaaatct aatgtaaaat
    12481 atacatctcg agtcccatct ctttcttcat aactaaaccc ttcaattatg acttccatat
    12541 ttatatttgt ttccgtaatt gtaaatctta atataaaacc ttccttcatc catttttcta
    12601 tcttatttac acaatcatat ggtgatggaa aacctgtata gtcacagaaa gtatagtctt
    12661 tatttggaaa aaaacttgat attgaaatgc ttttaagagc tacaccacca aaagttgcta
    12721 tttcacctat tttgagtata ctagaagagt ttacagcagc ttttccatta atactaaagg
    12781 aagatggaaa tactggaaat ctaaatctat cttcagcttg tctaagccac atttccatta
    12841 taagacaccc cctaactgtg ccaattggat acttgcaact aattttgatg ttatcttttc
    12901 tatatcagct tcttctctaa tcactattgt atcagctaac ttagctatgt ttatatttcc
    12961 accatttttt ctgcttctat attgatttgc ttcttgttta gttaaaacca tttcaccttc
    13021 atggagtctg gtttgatagt cattataagg aacatagttc aatccaaatg cattattacc
    13081 tttcttacct gaataagctt taccagaatc accagccttt gttccagcat atggattatc
    13141 tttgtaattt tttttactct tttccactag atttaaaact gttcctgtga tgcttccatt
    13201 ttttatctta ttaatcatag tttgccacca tttacatata tctttaacta ttttacatac
    13261 tttatctaat atgttaaaaa aagctcccaa tattggctct ataattttcc atccagtttc
    13321 caacagaggt cctaaggtct cccatacagt tttccaaata acacccagtg tctctataaa
    13381 attgtttatt tgaggtgcat attcacctaa aaaagtaaaa attcttgtga taataggact
    13441 aacagcttca actacaggtc cgattagttg gaatgctgta gttactattg taccaaatat
    13501 acttattaat ccttgtgcaa ttggtgattt attgtttata ctatctagta aattatttac
    13561 taagtttact actggaagta atgaatctat taatgctgta aaatcgatat tttgtaatag
    13621 tgtacttcct gcgttaacaa ttgcttttgg tgcattccct atcattgttg ctgcatgatt
    13681 ttcaaaacgt tcttgcttat tttcaaccat cattttacgc ttttcagcct cattagaaat
    13741 tcgatattga gtagaatcag aaaactcttt tccatttttt acacttctag attgcatctg
    13801 gtcatatttg ttgccaataa ttatcttacc attcattaca ttgcctattt taccagccat
    13861 tttttcagat aagccagcta aattgcctaa tccactttct ataaaagttt ttattttctt
    13921 tagtccttga cctaaaatat catcaaaagg tttaacaata ctcttcaagc cattgttaat
    13981 gcttgtttga gctcctgata ttatacctgg tattgtatta ctcatttctt tagctaaccc
    14041 catctgcttt acacttatgc cttccaatgc ttcatcaaaa gttttataat tttctcctac
    14101 cagagtatta agagattcca tgtttccttt tgctgcactt aagaaagctg ctgatacatc
    14161 ttgttcacta cttgtattca tatttaaagc tctaacatct accatagctt gagttatatc
    14221 tttttctttt ttgccaccag taatattact aatcatttta gctttagttc ccattgcaac
    14281 aacatcttca gggtcaaaag gcgtatcatt tgccattctt actaaactgc cataataatc
    14341 atcaacatct atttgattaa cttttccagt atttcttttc attacacttt ctagaacaac
    14401 tttctgattt tgttcctgac caagtaaatc aacacccttt ttcacagcga cacctatagt
    14461 agcaccattt gtcaaatcag atatcttccc ttttatcgaa ccaagtattc cacttgccat
    14521 atcctttaca gctattgcac cactccatac cttacctgca aataatctca atttgggaaa
    14581 tactttactt ataatgccac ttgccatatc ttttatagct attacaccac tccaaaaagt
    14641 atttttaaac aacattaact taggcaaaat tctacccata gcactactca ccatatcttt
    14701 aagattaata cttccttgat aaggacgttt ctctacttgt tttaattttc ccaagatact
    14761 acttatcact ctcttagtca tatcttttgc ttttattacg atattccaaa tcttgccacc
    14821 aatctttttt aaatttccaa gtaatttatt tttagttttt tctacttcat cacttttagc
    14881 tttaaatatt aatataatca taatatttga attaaactca tttttctttt gaatcaactt
    14941 attaaaataa tcaataatca tagctatagt tattgctagt ctcataatac ttccatttaa
    15001 acttccaata actcctgaaa cagcatttat gctagaactt aattttgtca tagttgaact
    15061 tacactacta gcagtagttt gaacttttct taacgaggaa tttaaactgt ttactctatt
    15121 acttgcactt ccaatagaat tttttatgct actagccatt acattagtgg aaagtgaaac
    15181 agccacagtt gctctcctaa aattatcagt tactgatata atttgattca tttttggtgt
    15241 gtaattatct gtaaggtaca tatttatacc ttcttctctc acatttccca ttacatacca
    15301 cctcctgtca aaggacacac tccatatcca tgtttcattt tttcaacttc ttcctcaatt
    15361 tctaaggcga aaaaagcttc aataagcttt aattcgcctt tatccattgc ataaaaaagg
    15421 gacggtctta tacctttttt cttccaataa tagtacatta ttgtagtcct ggtatccgtc
    15481 cctattagtt ttttacctct tctatcactg catttttacc ataacccata acatcttgaa
    15541 gtattctata taagttggta atttcacctg gtaataataa ttttcttgct aattcttttg
    15601 gtgttggagc cttaaattta tccattagct ccttattttt aaataaaagc tttccatcca
    15661 aatcacaaac accttctata attgtaagca tctgcatctc cggtaagtct atatccgttt
    15721 tcatattttc atcaatttta agacaagtat tttgtatttc ttcatcttta cttattgtta
    15781 aagctctaca cattacttta aattcctgcc caaatatagt tgataatctc ttaagttcaa
    15841 ctatagtact tggtctttct aagttttctg tatctgaatt taaaagtaaa tctactatgt
    15901 ttatattttt tgataatcca ttttcactca ttattatttg ctcctttatc aatcaaattc
    15961 aatcaactaa cttttttact tattaactat ctgaatttat taagtatata cttactaaat
    16021 atcagtataa aactaaatat aaaactaaat tatatcaaga aaatcatact cagtaaatgt
    16081 gaaaggagct tctatctctc cttttactcc aacctcccaa tcagctaaag ttaaatcatc
    16141 aaatgctata ttttttactg ctattctttc tgctccataa gaatctgggt catttaattt
    16201 tgatatagca acaaatctag gttcagaacc tctctttatc ttttctccaa ttaacttgtg
    16261 cattcttgaa ctaacgtggt atagagttat tgagcctttt cctttatatc ccatatactt
    16321 tgtatcagta cccatttgac ctgctattat aatatcttct tttgtaaatt ccatctttgc
    16381 ttgaaatttc tttacttctg ctactttgtt tccatcaagc caaagttctc cccaagtacc
    16441 actcattaca tttctagctt ccatatttgc catattaata atcctcctat atttctattg
    16501 ataaatctat atcttccata gcatcaagta cttttatttt tgcttttaaa aatactttag
    16561 aacctgtgtt agcttctttt atttcttgta atgtcatata agataaatct actccttttg
    16621 attttaaata cgatttttgt gcttcaaaat ctatttcaac agtagaatca gattctataa
    16681 gtgctgattt ttctaattct tctaaataac tttttatagc tactatcaat aaacatttgt
    16741 tgtcataact gttagtaacc tttcctatat agtcatctat tatcaccttt cttatgtcac
    16801 tatgtataat atctaaagtg tcaactattt ttattttctg gaacatttct cctttttctg
    16861 ctgttaattc agttaaagaa tttactcctc tagcaattct tatagctcct gcttctttaa
    16921 taagtataag ctctccttta ttaacccttg attctgcatc aactttcgtc atcttaggta
    16981 tatcgactac atcactaagt ttagtataag ttactgattg acttaaaggt gtacctgcta
    17041 taagtccagc caccctgctt gtaaactcat caacactgta tttcttttct ccaactaaca
    17101 catcttctgt agtaaaatta attatccctt catgatttcc tacaactttt cctaatacag
    17161 ctttaacctt aaccttatct atatctctaa gtttaattat ccaatttttt atagcagtct
    17221 tatcagcttc tactgctttt ggcatacata gataattaaa ttccttagtc tctaaaaaat
    17281 ctaatgcagt ttgaatatct gcttctcctt ctattacata aactaataat ttatttggag
    17341 tgttaacatt tcccatcaaa gctaaattta tatattcttt attttcagca gataaatcaa
    17401 ctggtatatc ctctttttca tgtatttcat taagacctag tgccttagca tctttaagta
    17461 ccattgcaat tattcctcta gctgaacgtt ctttaacagt tgtagctagc tccttaaatg
    17521 atatgttgat acttggtaat cctatagcca tactatcact ctcctaaatt taaattgatt
    17581 tctttcatat tttcatatac tacatcttct tcatatacac tatccagata gtttagtgta
    17641 aatgtaaagt tcaatacata aattgactct tcttttttaa tactttgttc tagctttgtt
    17701 atatttaatt ttctgtctgt tacctttata tttctgttaa atagcttatt taattcatct
    17761 gaaatttcat acattctaat actctttgac tgtggttttg gtaagtattt tatagagata
    17821 acaatatctt tgttattcgt atttaacatt acaggtgtac aaataactga tagtatactt
    17881 acaaaaaaac aagacccttc agagccttgc acactttcat catcaataaa aatactttca
    17941 gtaaagtttt ttttgagaat ctcaataatt gtttctaata tttccttgta tttaagcaaa
    18001 taatcaccac actatcacta aatattcttg cttaatctga taatcttact ataaatgata
    18061 taagtcattt ttaaatatat atctcattta ttcctgtcaa gtatcttcta tatttttagg
    18121 taatattatt cttttcaatc tatcacctcc ttcaatatgg atttctttca caataacatt
    18181 atctcacata ttttattgcc tattgttgca atggtgtata taaagtgttg taaaattgtc
    18241 ctatttttta taatatctca ctcaatttaa atatcatctt tttctttaaa ttgtagcaag
    18301 atgttctatc taagtgtagt ttcatagaaa tatatgtcat attgtttttt gtcttactat
    18361 tataaaaaag attaaaaaat tccatttcta tatcatttag acacgttaga gcattttcta
    18421 tcttctcttt ttctatttct atatcttctt tttccatttt taattttctt aatctttcct
    18481 ctctttttaa cacctcattt tccacagaag agcttatgtt atatgtgtta ctagtctttt
    18541 ctgtatatac cattgctcca catccagagt attcattctc acatcttttt atgtccaagt
    18601 taatattctt tatctttata tccaaatact tataattatg taatctttta tctgtttctt
    18661 taaataatat atcttttttg ttttccatag tacttcccct tttttatata cttattatta
    18721 gcttcaaatt tacaataatc tctttagtgc acagtttttt actaatctca caactttcaa
    18781 gaacatattg ttctataact gtataaaaaa aacttcttct atattttgtt ctaaaatact
    18841 agaaatcttc ttagccataa gaagtccagg attttttata cctctttcta tttgagatat
    18901 ataatcattt gtaattccag cctttctggc aagttgaatt tgtgtcatac ctttgcattt
    18961 tctataatat ttcaagttat ttttaaacat tgttttatac ctccattact attaagaata
    19021 ttttgttctt cctcacttag attatataga attatttgtt ctttatcaat agattttaga
    19081 aaaaaatatt cttttatatt ttattagttt tattttttag aacaatttgt tataatctat
    19141 acataaggtg gtgttataat gtttgccaaa agattaagag aacttagaaa ggaatttgga
    19201 ttgactcaaa gagaacttgg agaaaaagta ggcgtttccc aaagagtctt agggtactat
    19261 gaaacagaaa acagatttcc tgatgaacac attttaaata aattggctga tgtatttaat
    19321 gtatcagttg attaccttct tggaagaacc ttagttaagg aaaatattga tacagtagct
    19381 gcacatagaa aaaatccaca tgaagagtta cctgaagaag cacaagaaca acttaatgat
    19441 tatatagaat ttttactaaa taaatataaa aaaaaataaa ccatatgatg agcagtctat
    19501 ttttctgctc ttttaatata atttaaaggc aaacatacat tctattttag ggggattttt
    19561 atgaataatt tggataagct ttttgaatta gcttctcaag aagagataat aattcattat
    19621 accacttata ttgcaggtga tttagaagga ctatatataa acaaacatgg tattaaaatt
    19681 atatcattac tcagtaattt aaaacaaaac tcaaaaaaat tgacatccat cttagctgaa
    19741 gaacttggac atcatttcac tagccttgga tactatgtat cctcttataa cgattattat
    19801 acaaaaatta ttatagataa gtgcgaaaat aaagccttaa aatgggcttg tgagttctta
    19861 attactgaag aagatataat aaacataatt aactctggca tcacttgcgt ttatgaaatg
    19921 gcagacatac ttaatgtgga tattactttc tttcaaaaaa gattagaatt tctatcatta
    19981 aaaaaacagt ctttacaact tggaaataat aaatatttaa tattaactaa tttgccgtat
    20041 ttctacatat ttgatccaat ttcataataa aaatagtagt aattttaa
//
LOCUS       DACULQ010000060        19641 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.060, whole genome shotgun sequence.
ACCESSION   DACULQ010000060 DACULQ010000000
VERSION     DACULQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.060"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(128..1318)
                     /locus_tag="JA154_08695"
     CDS             complement(128..1318)
                     /locus_tag="JA154_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HAT7995944.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKISYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(1578..2009)
                     /locus_tag="JA154_08700"
     CDS             complement(1578..2009)
                     /locus_tag="JA154_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAT7995945.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            2122..4350
                     /locus_tag="JA154_08705"
     CDS             2122..4350
                     /locus_tag="JA154_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAT7995946.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(4461..4946)
                     /locus_tag="JA154_08710"
     CDS             complement(4461..4946)
                     /locus_tag="JA154_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7995947.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            5198..6421
                     /locus_tag="JA154_08715"
     CDS             5198..6421
                     /locus_tag="JA154_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAT7995948.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            6425..9559
                     /locus_tag="JA154_08720"
     CDS             6425..9559
                     /locus_tag="JA154_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcrB/AcrD/AcrF family protein"
                     /protein_id="HAT7995949.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSREKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            9581..10891
                     /locus_tag="JA154_08725"
     CDS             9581..10891
                     /locus_tag="JA154_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAT7995950.1"
                     /translation="MNKKMSKIVAISCGVGIITANLIPVYADNNTSSINKVDNSILSD
                     SNKRTSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFP
                     YDQIGLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMK
                     LKKDLGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEY
                     RFESFRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVA
                     PTKPEQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFN
                     TENGLVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGL
                     ITLTDYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(11180..11449)
                     /locus_tag="JA154_08730"
     CDS             complement(11180..11449)
                     /locus_tag="JA154_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995951.1"
                     /translation="MSLLEGIKLTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCP
                     EPSCRALVSRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(11639..14266)
                     /locus_tag="JA154_08735"
     CDS             complement(11639..14266)
                     /locus_tag="JA154_08735"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088922.2"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HAT7995952.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(14327..15169)
                     /locus_tag="JA154_08740"
     CDS             complement(14327..15169)
                     /locus_tag="JA154_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase regulatory protein"
                     /protein_id="HAT7995953.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(15176..15814)
                     /locus_tag="JA154_08745"
     CDS             complement(15176..15814)
                     /locus_tag="JA154_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7995954.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(16250..17542)
                     /locus_tag="JA154_08750"
     CDS             complement(16250..17542)
                     /locus_tag="JA154_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAT7995955.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(17579..17935)
                     /locus_tag="JA154_08755"
     CDS             complement(17579..17935)
                     /locus_tag="JA154_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HAT7995956.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(17970..18329)
                     /locus_tag="JA154_08760"
     CDS             complement(17970..18329)
                     /locus_tag="JA154_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995957.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(18398..>19641)
                     /locus_tag="JA154_08765"
     CDS             complement(18398..>19641)
                     /locus_tag="JA154_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAT7995958.1"
                     /translation="FDLLKSYDEYFIAREIKHIISGMFECTLNDEEIIYLTIPLVSGN
                     APASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLGYHLSFTLNRLLF
                     NIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYIAIHFSGYLERNS
                     SRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLRNINLDEYDIVFT
                     TIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNSTNLLINNLLDEDK
                     FMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFDKGLLFPHSVNEK
                     SDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYEEVLKIGQDVKLT
                     NKISKCRTFLEFKKVLLKNLI"
BASE COUNT         6783 a         3212 c         2610 g         7036 t
ORIGIN      
        1 aataaaaagc aggtatcaat caaatagtaa catgtattat actatacatt actatcaata
       61 ttgtacctgc tttcatatta taagcaataa agaaaaatct atttattagg ttttataata
      121 aaaacaatta taaaactctt cttgcattta catatctact tgaatagtag ctactattta
      181 tattagatat tgttactttt tttgatgaac ctgaagatgc atgtatcata tctccatttc
      241 ctatgtatat tccaacatga cttacagaac cattatttga accttgtgta tcaaagaata
      301 ctaaatctcc acttctaagg tctcctctat ttacatatgt tccatactta ctttggtctc
      361 tagatactct tggtatacta actcctacag attttttcat aacgtactgc gtgaatccag
      421 aacagtcaaa actatttgga ccttctgctc cccatacata tggttttcca agtagtgtct
      481 ttgcaaaact tattacacta tcagctcctt gacttggaga attagtcgaa taagatggaa
      541 ttgaagtatc aacagaacct ttttcagcaa ggtattgact tgacacatat ccagtttgat
      601 tgccataact tatttttgtc catcctccac tttcagaaat acttccaacc tcagtaccat
      661 aacttaatgt agctatttta gaatgacttg tacctggtcc tgttctaacg ttcaatgact
      721 ttgctgttac aacctttgta ccctttacaa ggtcatttga actgttttca gcattttcat
      781 ttgtagagtc atttacacta gttcctaaat atttacttga aacataacct actctaccat
      841 tagaacttat ttttgaccat ccatttgact cagatatgta tccgacttca gtcccatatc
      901 ctagtgttga tattttagaa ctacttgtac ttggtcctgt tctaaaattt aatccttttg
      961 ctgttacaac ttttgtcgac ttaactgaag tatcactact ttcattgcta cttccaaggc
     1021 tatttattgc tacataattt ccatgcacat aacctgtgtt tccattatat ttaactttta
     1081 cccaagaacc ttcttttcct acgtactcaa ctttatcacc tttgtaaagt ttccccattg
     1141 attcatggtt tgtacctggt ccttttctaa aatttaccgc attccctgtt attgttctgc
     1201 tttctatact cacactctct acttcagact cattagcata tgctaaattc cccatagtag
     1261 gcattattat cgcagttgcc attatactgg ctgctattct cttttgatta aaattcataa
     1321 ctttccactc atctcctctt ttctactatc tttatttcac ttcaaaatat aaaagaaata
     1381 attgacacta tcaaaatatc aattatttct tttatattaa tattattaca aatttgtaac
     1441 tatttgtcta tagtaatatt tgcttaccat aaattaccat gtataaattt taatctttaa
     1501 tagcctgtaa tttagtaata tagccatctt ttaccttttt taaaaatggc tatatttttt
     1561 aacttttttg taacttttta aaatccagtt gagccaaacc caccttttcc tctttcggag
     1621 tcacttaatt cttttacttc ttctatgtta atatcataga taggttttac tatcatttga
     1681 gcaattttca tatgcttttc aaccttaaaa tcatttttac catgatttat taatataatt
     1741 ttaagctctc ctctgtatcc ttcgtcaata gttcctggtg tatttaatac agtaacagaa
     1801 tgctttaagg caagcccact tctaggtctt acctgcgctt ctgtcatagt aggaagctct
     1861 atacatatcc cagttttgat aagtttagtc tctccaggag gtattacaac ctcttctata
     1921 gaatacaaat ccataccagc atcacctttg tgagcaaaat taggtattat agcatcatca
     1981 tttaattttt ttacttttaa attgtacatc ttatatctcc tttcaacttt ctataaatat
     2041 tataacttaa atttcgacct cttgaagttt tttatttttt tgtggtaata taattattag
     2101 tgttttaaag gaggattttt tatgaaactt gtacttgctg agaaaccatc tgtggcaaaa
     2161 actatagcat catttttagg tgcaaagacc agacaagatg gttactttga aggaaatgat
     2221 tatataatta cttatgcagt tggacactta gtatcattat atgatatgaa ggattatgat
     2281 aaagataaat actctggctc ttggaaaatg aacaatttcc catttgttcc tgaggataag
     2341 tttaaattta agattgatag ttctaaaact aaacaattca acactgtaaa aaagcttttg
     2401 aatagaaaag atgtagaata tgtaataaat gccactgata atgaccgaga aggagaactt
     2461 atagcatttt taatattttt actggcaaaa aataaaaaac cagtaaaaag aatcttggta
     2521 aatgaatgga caccagaaga tattacaaga ggtataaaaa acctaaaaga tgaagatgaa
     2581 atgagaaatc ttcaagctgc tggatatact aggcttatta ctgactggtt gattggaata
     2641 aatttcactt ctgttgctac attgaaatac ggaaatggga aactattaaa cataggtagg
     2701 gtaattcttc caacagttaa attagtctat gatagagata tggaaatact aaactttgtt
     2761 cctaaaactt attatgaaat agaagggcat tttaaagctg aagctggtga atataaaggt
     2821 aaatatgtta agggtaaaga aagtaaattt gatacacttg aagatgcaaa taaaattata
     2881 gcttcaatta catctgaaac tggtaaaata ctagataaaa aagttactat gtcaaaggaa
     2941 tatgcaccaa aactttttag tttgacatca cttcaaggat atataacttc taagtattcc
     3001 aattttacat ctgataaggt tttaaatgtc tgtcaatctc tctatgaggg taatggtaag
     3061 ggtggataca taacttaccc tagaactgat tccattttct tagaagaaag tttggcttca
     3121 aaagcttctc aaactttaga taaattaaag gttggtcttg aatatgaaaa taaaattaaa
     3181 ttttctaaaa ctaaaagagt ttttgattct tctaaagtag actcccatag tgctataatt
     3241 ccaacttata taattccaaa aagtttatct aaggatgaac aattagttta tgatgccata
     3301 aaagatagat ttgtagcaaa ctttatgcct cctgctgaat atgagaatac agaaattaag
     3361 actgaagtag ataattgtac tttcttaaca aaaggtaagg tgcttaaatc aaaaggatac
     3421 cttgaggtat ataataagga agaaaaaaac gatttacttc cacttgtaaa taaagatgat
     3481 gttgtagatg tgttagaaat aaaaccatta actaagcaaa ctactccacc taaaccgtat
     3541 actgaggaca cacttttaaa ggctatgaaa aattgtggta aaaatgtgcc tgaagaagat
     3601 acaacagtgc tatctggata ttcgattgga acatcagcca cacgtgctga tgtgttaaag
     3661 aagataagtc aagtgggcta tgtaaagaaa aaaggtaaat cttattctat aacagacctt
     3721 gggaaaaatt tagttgaaat ttttcctgtt aaagacttat ttgatgtaga ttatactgga
     3781 aaacttgaaa aaagtttaag cgatattcaa aaaggaaagt atacaagaaa agaatatctt
     3841 actaatatca ttagttttat atatcaaaat gtcaatatta taaaacaaga tgcttcaaaa
     3901 aatataaaca cagaagcata tactttcgat tcaaaaacta aaaaatttac taaaacaaaa
     3961 gaaaaaaaac agaaaaaaga tgtaactaat actgaaaaag aagttgctat atcaaaggat
     4021 tctaataaca aaaaagaagt taaaaatggt gatgaaaatc aatctcttgg caaatgcccc
     4081 atttgtcaag gtgatgtact agaatttgac aaaggctttg cttgtaaaaa tcataaagaa
     4141 tgtaaatttg ttatttggaa aaatgataag tatttaagtc tttataaaaa gaaagttaac
     4201 aaaacaatgg tcaaaaatat tttaaaaaaa ggtgaaacta aagtaaaatc tttaactgct
     4261 aaaaacggta ataaatttga tgctacatta aaatatgata aaaatccaga tactggctat
     4321 ttcaattgga aaatagagtt tgacaactaa atattcataa gaaaagaaat agcagtagta
     4381 taataatcta tattatatta ctgctatttc ttttcttatg aagtttcaac ttttttaata
     4441 gtgactttta caatcacact ctaacctaat ttctttacta tttctgataa ctttaatgta
     4501 tgttcatcta ataaagttag ttcctcttca gaaagattag ataatttttt tactagagat
     4561 tctttgagaa tagcttttac tgcatcaaat aatttaaatg ctttatctgt taattcaacc
     4621 ctaagaatcc ttctatcttt tgggtcagga aatttattga ctaaaccatg ttccactaat
     4681 ctgtcaataa tcggagtcat atttgatttt aaaattccta aattagaagc tatttgagaa
     4741 attgatgatg aatttgatgt cataagataa agaattacct tagcatgtga tggagccatt
     4801 ggacattctt caaaataagc tttcattata tcagagttat tttgaaactt ttttaacaaa
     4861 tcgctttctt taaatatatt attttcaagc acaaataaaa aatctaaaaa agagtccgct
     4921 attttaagta tattgttgtt atccatattt gtaacatccc ttcaatagat aatataataa
     4981 aacattgttg tttaatatag tataaattat aaactattaa taaatttaaa acaagaaaaa
     5041 gttattgact cataataatt ataaatttat aatcaaatta gagaattata aattgccaat
     5101 aaagggaagt tttttaatta gtctaaaact aaacttaaat tataagacac tttaaattct
     5161 acaacaagct agtgtgcaat atataatagg aggagatatg aaaagatatt taatagtttt
     5221 cttaatgtgt agttcactgt ttttagttgg atgtggaaaa acagaagaat ctgaacctga
     5281 gaaaccaata gcagtttctg ttcaaaaagc aataggtgga gaaattgaaa acacaaattc
     5341 tttctcagga actacaaagg ttaaagatga aacagctgtt actgcacaga ctgttggcac
     5401 agttcaagaa gtgtatgtaa aacttgggca aaatgtaaga aaaggagatg aattattgag
     5461 tataagcagt ccagagctag aaaatagtgt aaagcaaagt aaagcctcac tagatttagc
     5521 aaaagcaagt tattcaagtg ccactggtgg ttcattagag gcacaagtaa atcaggcaaa
     5581 aacagctctt gacaatgcaa agattcaata tgaggagtct caaagaaatt atgacaataa
     5641 taagattctt tatgaacagg aagtcattag tttagaccaa tttaaaaaaa tagaattttc
     5701 tttagagcaa acaaaacaac agttagattc tgctcaaaga gcatacgata cagcaacttc
     5761 aaaaagtata cctcaagcta aagcacttgc taaaaaacag cttgaccaag ctcaagtatc
     5821 atataattta gcaatgagta atttagacaa attaacatta acatctccag tagatggtac
     5881 aataacagct aaaaactttg attcaaagga aatgataaca caatcacaac cagcttttat
     5941 tatttctaat cctaatatac tagaagttga tttaaatgta gcagaatcag atataggtaa
     6001 atttaagaaa gatggaaatg tagatgttat aattgaagac caaaggattt taggaaaaat
     6061 agattatgta ccttcagtag ttgaccctca gacatcttta tatcctgtta aagtacttgt
     6121 aaataataca aacaacaaat ttaaggcagg tatgtctgct caagtaaact taagtgttga
     6181 aaaagaaaat ggtgctgtta cagtacctaa aaaagcaata tttgaagaaa atggtaaaaa
     6241 atatgtatac atagcaacaa aagacaatat agcaaagaaa cacttggttc aaactggaat
     6301 tgtaacagag gataaaatag aaattaaaag tggtgtaagt gataaagata cagtgattat
     6361 cggagggata agtcttattt ctgacggaac taagatattt cctgtagaaa aggagaagta
     6421 gactatgaat ttaacacaga cttccgttaa acgtcctctc acaatcataa tggtcttttt
     6481 agtattaata gtttttggtg gtattggata taaaaaaatg tcaataaatc taatgcctga
     6541 tatagaaata ccagttgtaa tggtcatgac tacttggaca ggtgctggtc ctcaagacgt
     6601 tgatgaacaa gttagtcaaa aagttgatga aagtttatct gccgtttcaa atgtaaaatc
     6661 tactatatca agttcacaag aaagtgtttc tatggtagtt gctcagtttg aatttggaac
     6721 taatttggat gaaataatga atgatgttag gtctaaagtt gatgcattac agaccagcct
     6781 cccagatgat gcagctaaac caacagtttt aaagctggat atgaatgcac aggctatagg
     6841 tcaacttgtc atatctggag gaaatgaaaa tagttcacaa gccttaagaa aatacgctga
     6901 agatgtaatt cagcctaaaa tagaatccat agatggtgtt acttcagcag accttaaagg
     6961 tggagaaaaa gcacaggtta atgtaatagc tgacccagct gttctttcaa attatggagt
     7021 tagtttatcc actataaaag gtgtcttatc ctctagtaat aaaacattcc cttatggttc
     7081 aattactcaa ggtgaggata aaatagtttt aagagcgatt gataaattag aatccctaga
     7141 tgatataaaa cagattcaaa ttcctgtaaa aggaggaaat acagttagat tagataatct
     7201 ttgtagtgta gattatggat atgcagacaa agatagtatt tatagataca atggaaaaga
     7261 cagtttggta atagatattt ctaaacaaca agatgccaac actgtaaaag ttatgcagag
     7321 tgcatacaaa tacgtagatg aattgaataa ggaaaatcct caatttaaaa ttaaaattgc
     7381 taatgataca agttcataca taagtgaatc aataaacagt gtaatgagca acttgcttat
     7441 aagtgctgtt atcgcattta ttgtaatatt tgctttccta aaaagtgtaa gagcttcact
     7501 agttgttgca gttgcaatac caatttctat aataggtgct atagcaattc tctactttac
     7561 tggtgaaagt ttaaatctta ttactgcaag ttcattggta atttcagttg gtatggttgt
     7621 tgataatgct acagttgtaa ttgaaaatat atttaaatat aggaaaagtg gtagattgag
     7681 tcttgatgat tgtgctattg agggaacaag aactgtaaca aatgcaatac ttgcatcaac
     7741 attgaccaca atagcaatat tcttacctat catgtttaca gaaggtatag ctaaaataac
     7801 ttttggggca ttaggtaaaa cactaatagc ttcactagca ttctccttta taggtgcaat
     7861 tacactcgtt cctagtgtat ttgcaaaatt aagcagagaa aaaaatagtc aaaagatgca
     7921 agaaaaagat agtcctatat ttgataaggt aagtgaagca tataaaaaat tattaagtgt
     7981 atgtttgaga catcaacgct tagttgtaat atcaagttta cttatttttg tagcttcctt
     8041 atttggaatg ggatttatag gtatggactt tatgcctgca gcagacaagg gtgaattatc
     8101 tatttctata gaacttccaa aaggtcttag cctagaatcc aatgattact atgtatctat
     8161 gactgagaaa aaagtctcag atattccaga aattaaaact ttaataacaa ctttgacatc
     8221 agattcaaat aatagtaaag caagtatagc aattgaatta gaatctcaaa aaaatagaaa
     8281 aagaactact gatgaaatag aaaaagagat aattgataga gtggctactg taccagattg
     8341 taaaataaat gtaagtcaaa acagtagtgt tatgggtgga gctggaggag cagacttcac
     8401 attagaaatg aaaggtcctg atttagatgt attaaaaata ctgggaaaag aagctgaaaa
     8461 agaatttagc tctataaaag gatttagaaa tgttgaaact tctcttacag atacaacaca
     8521 agaagctcaa tttactatag ataaacataa ggctcaagaa tatggtgtaa ataccgctga
     8581 aattgcaggc atgttacgta cagcaataaa tggtgactcc gtaaccactg ctactattga
     8641 tgattacaaa gtggatgtaa acctaaaatt taaagcagat actattgata gcttagaaga
     8701 tataaagcaa ctaaaagtaa actctgcaac tggaaaagaa gttcctcttg gtgccttttc
     8761 agatataaaa atggcagatg gtctacaatc tatatctagg actgatggag atttttctgt
     8821 aaatataact gctacacttg acaatttaga tgctggtact gctacaaaac tagcactaga
     8881 aaaaacgaat aaaatggtaa ttcctaaaga ttactctata ggtgttggtg gtgaaacaga
     8941 aatgatgaat gaatctatgg caagtcttgg attttcaatg gtcatagcta tagcattagt
     9001 ttatatggta atggtagctc agtttgaatc atttagtaaa ccatttataa tcatgttctg
     9061 tgttccattt gcattcgttg gagttgtgct ttcgcttcta gttagtggtc aaagtttaag
     9121 tactgtcggt atgctaggta taatcttact tatcggtatt gttgttaata atggtatagt
     9181 tcttattgac tacatagagc aacttagaaa aacaaatcgt aacaatgatt taattggttt
     9241 ggtttcaatt ggttgtgcag caagattaag accagtctta atgactacag caacaacaat
     9301 tttaggtatg ttgccaacag cactatctct tggtgaaggt ggagaaacta tgcaaccact
     9361 agccatagtt ataatgggtg gtctaactgt atctacactt ataactttgg tgcttatacc
     9421 tactatatac ttaatatttg ataaatggga aaataagttt tcagacaaaa tgcctaaaat
     9481 atttggtaag ataacagata aaataggaag tataaaacgt aaaataataa agaaaccaaa
     9541 attgaaagct aaagtttaaa tagaatggag ggtgattgta ttgaataaaa aaatgagcaa
     9601 aatagttgca attagctgtg gtgttggtat aataactgcc aatttaatcc cagtttacgc
     9661 agataataat acttcttcta taaacaaagt tgataattct attttaagtg actcaaataa
     9721 aagaacatct gaaaaggaaa ttttgactgc tagagaagct gttagtgctg cacttaaaaa
     9781 tagtgaaaaa ttaaaaatga aatcagaaga aataaaaatg ttaaaagaaa agttagaagt
     9841 acaagatgag tttgatagtt ttactggaag tgacaactcg tttccatacg accaaattgg
     9901 acttcttaaa aatcaaagtg aacaggctaa aggctttatg gaggaccaaa tagcaaatga
     9961 tattactaat aaatttaacg atttagtttc aagagaaaat gagctagata aaataaaaaa
    10021 caatcttgaa ataaaaacta aagaaattaa agatatgaaa ttaaaaaaag atttagggtt
    10081 agtaacatct ttagaaactg aatcagctga acttgaactt caaacactac aaaatactca
    10141 aaaagctaaa cttcaagaac taaaaaacaa tcaagactat tttaagttat taactaatat
    10201 agatttaaat aattatcaat tggataaaga atatagattt gaatctttta gagtaggtgg
    10261 ctcagttgat tcatatatgg aaggtaaggt taatgaatat ttaaaatacg accaattgat
    10321 tttagaacgt acagaagaaa gctttaatga taaagatgag aacaaagcag atttaccaga
    10381 tagacctaac cttacaagac cagttgctcc tacaaaacca gagcaaggta acctaactga
    10441 tgatgaatac aaggttctta tggataagta tgaaaaagat tatgatagat ataaaaaaga
    10501 agttgaagca tacaatcttg aaagacagac ttatgctgct ggccttacta cttatgcaaa
    10561 ttatcttcaa caaaaattta atactgaaaa tggtctagta acattagaag acagtaaaaa
    10621 agctcttaaa aaagggttaa ttgatagcta tgctcaatta cttgctttag aagatacaat
    10681 tcagattact aaaaaacaac ttgatttatc agaaaaacaa ttaaaaaata ctaaattaag
    10741 atatgattta ggattaatta ctctaactga ttataaaaaa caagtggtaa gtaatgaaga
    10801 tgctaaaaat agctatgaca ctcttattgt gaattataac tcactaaaaa atggtataga
    10861 aaaaccttgg atacttaata ctggaaaata attctggtaa atagaaagat tactttaaca
    10921 tacgtttgat taaaaaatag ttatgcatac tcgtggcttt agtcatggaa gattcagaag
    10981 gcaaataata gaaatagatt agtaaaaata tatttactta taaaactgta tacttttata
    11041 gaggctatct caaaatagag ataagcctct tttttattgt ttaaattata aaaatatatt
    11101 tgattccata caaaaagagt gccttctgaa ctaaaaagtt cattttgaga caccctcaaa
    11161 agcttaactc taaatttaat tattttttat caaatgtttc atctatttta catctcacca
    11221 tgttaacaaa ttcagaaata ttatatcctc taagtttgca tccacattta ggacaataat
    11281 ccagtttcct agataccaaa gccctacaac taggttctgg acatcttatt tttagactct
    11341 catctgctac tttaaatcca catcgtgggc atatactacc tggacgtgat gatacttcct
    11401 ttccacattt aggacacttt gtcaacttta tcccctccag taaactcaat ttaattgata
    11461 gttgaaattt ctctctacaa taatatatgc aatataatta aatacgtttc acttacaact
    11521 atataagtta aatttttata ataaaaatcc cctatattca atttacaaat acttaatttg
    11581 atacattgaa caatagggga ttttattttt atttagttac atattttgca gtctattatt
    11641 atctaggatt ttttatagat gcttgagctg ctgcaagtct agcaattggc actctaaatg
    11701 gtgaacaaga tacatagtta agtcctacac tataacagaa ctctactgaa gatggttctc
    11761 ctccatgctc tccacatatt cctagtttta attctggttt aacttctcta cctaatttag
    11821 ctcccatctt aactaactta ccaacaccat tttggtctag tacttggaat gggtcttttt
    11881 ctaatatttc ttcatctaca tattgaccta agaatttacc agcatcatct cttgaatatc
    11941 caaatgccat ttgagttaag tcattagtac caaaactgaa gaagtctgct tcttgtgcta
    12001 tttcatcagc agttaaacaa gctctaggta tttctatcat agtacctaca gtataaggaa
    12061 cttctatacc ttcttctttt attatagcgt caacagtttc tactatcatt tttcttatta
    12121 tttttaactc atttacttct ccaactaatg gaaccattat ctctggagaa actttaacac
    12181 ctgctttgat tgcttctata gcaccttgta ttatagcttt agattgcatt acacatattt
    12241 caggataagt tattgcaagt ctacaacctc tgtgtccaag cattgggtta aactcatcta
    12301 aatcaactat tctcttctta atatcactta cttttattcc catagatttt gaaagttctt
    12361 ctatagtctc atcatcatga ggcaaaaatt catgtagtgg tgggtctagt aatcttatat
    12421 taactggttt tccatccata actttaaata actctacaaa atcctctctt tgcattggta
    12481 atattttttc aagagctttt tctctttgtt ctactgtatt agataatatc atttctctaa
    12541 ctgctggcaa tctagcttca tcaaagaaca tatgctcagt tctacataga cctatacctt
    12601 ctgcaccaaa ctctatagct gcacgagcat ctctaggatt atctgcatta gttctaacca
    12661 tcatacattt gtgtttgtct acccagttca ttaatttttc aaaatttcca gttaaagata
    12721 cttctgtttt cttaacatct cctaggtaca catttcctgt agaaccatct atagaaatgt
    12781 attctccttc ttttataact acatcatcaa gtgctcttat ttctttatta aactcatcaa
    12841 cttttatttc tccacatcca gcaacacaac atttacccat acctctagca acaactgctg
    12901 catgtgacgt cattccacct ctagctgtaa ggattccttc tgctttaacc attccttcta
    12961 tatcttctgg agatgtctct agtctaacta aaactacttt ttctcctttt tcatttgctt
    13021 ttactacatc atcagcattg aaataaactt taccacttgc tgcacctggt gatgctggta
    13081 aaccttttgc tataaccttg gcttctttta atgccttatc ttcaaatttt ggatgtaata
    13141 attggtctaa ttgattaggc tcaattctca tgatagcctc tttttcatca attattccag
    13201 cttcaactaa atcaaccgct acatttattg ctgctgctgc tgttctctta ccatttctag
    13261 tttgtagtat atataatctt tcattttcta tagtaaactc tatatcttgc atatctttat
    13321 aatgaccttc aagtatatga gtaattttta caaattcatc gaatacagct ggcattacat
    13381 ctgcaagagt tgatatattt tgaggagttc ttatacctgc aacaacgtct tcaccttgtg
    13441 cattcattaa aaattcacca aataatttat tttctcctgt agctggattt ctagtaaatg
    13501 caacacctgt accactagtt tctcccatat taccaaatac cattgattga atatttactg
    13561 ctgttcctaa gttatgagct atatcattca atttacggta tacgattgca cgaggattat
    13621 tccatgatct aaatacagcc tctattgcaa gcattaattg ctcttttggg tcttgtggga
    13681 aatcattttt tatttctttt ttataaattg ctttaaattc ttttactatt tcttttaaat
    13741 catcagttgt aagttctgta tcaactgtgt atccctttgc ttcttttact ctgtctaata
    13801 cattttcaaa tttatatttt ggaacttcca tagcaacatc agaaaacatt tgtatgaatc
    13861 ttctgtaact atcatatgca aatctttcat tttgtgtagc ctctgctaag ccaacaacac
    13921 tattgtcatt aagacctagg ttaagaattg tatccatcat tcctggcata gagaatactg
    13981 caccagaacg aactgatact aataatggat tcttattgca tcctaatgtt ttatttaagt
    14041 ctttttctaa agtagctaaa tgctcttcta tttcttttat tatctctgct cttatacttt
    14101 cattgttaac ataataatca ttacatgctt ctgttgttat agtgaatcca ggagggacag
    14161 gtaagcctat cttagtcatt tctgctaaat tggcaccttt acctcctagt aaagatttca
    14221 tgtctttgct tccttcacca aaactataaa catacttagt ttccattgtt aaatccccca
    14281 ttaatctttt tattttaatt tatatcattt ttattaatag acattattaa atttctattt
    14341 ctttgtatat ctttagacca ttttctttca ttatgtctaa aattatacct gctgtttctt
    14401 ctattgcctt atttgaaacg tttattactg ggcatccaat tcttttcatt atctcttctg
    14461 aataatctaa ctcctgaagt attctttcta aattagcgta gtttgcttta ctggacaatc
    14521 ctaaagcttt taatctttgg gttcttatct cattcagctt ttcaggtgaa ttagtaagac
    14581 ctattatttt tttagtatct atctcaaaca cttctttagg aattggtatt tctggaacta
    14641 atggaacatt agctactttt atatttttat tagctaaata catactcaaa ggagttttag
    14701 atgttcttga tattcccact aaaattatgt ctgcttgaag aacacctcta gggtcttttc
    14761 catcatcata tttcactgca aattctatag cttcaactct cttaaagtaa gattcatcta
    14821 attttctaat tataccagct tctcttttag gttttcttcc tgttctcttg cttatttctc
    14881 ttagtatatc tgtcattaaa tctatacaac taagaccttc tctttcgcaa tatctttcaa
    14941 caatactgca taattcttca tcaactaatg tatatacaat taaagcgttt tcagcttttg
    15001 cattctccaa agtttcaagt agtactcctt tattaatcat gtatggaaat ctttttattt
    15061 cataagtttc attcacataa aactgagaca tacatgcctt tgcaacttgt tgtgcagtct
    15121 cccctactga atctgatact gcatatataa ttaggttttt caccagcata cctccttaat
    15181 tgtcaactat ttctaagaat agcttagtta tgttggtttt tgatattctc ccaacaactc
    15241 tcatatgatt tttgtcttct cccttatatt caacaatcgg aatcgaatct acttcatgtt
    15301 ctattatttt tcttgaagct aataaaacac ttgctccttt agttaaagtg actacatttg
    15361 gagttcttgt cattatcata cctattggca ttttatttat atcagaacct cctatagttg
    15421 cttttagcaa gtcttttctg gaaacaactc cacataactc ttcattctca tctataataa
    15481 ctatactccc tacatcagat aaaaacatag tgactattac atcatatata ctttcatcct
    15541 tcttagctag tacaggcatg ctcattatat cctctactgt tttttctttt acacttttac
    15601 caattaaatt tatttcacta actcctgaat aaaaatatcc aacttttggt cttgcgtcta
    15661 atattcctgt cattgttaat atagctaaat cagatctaag tgtagcacga gtcacattca
    15721 agctggaggc aatactttca ctcgttattg gttcattttc cttaactata tctataattt
    15781 ttagttgtct ttcattaagc tgaataataa tcaccttctt tcattgtgct atacttttta
    15841 tcttttcgca tcttatatag tatattatat aactatatat accattctgt aaacagattt
    15901 tttattgaat acgatatatt ttttactaag ttctctcgct taaagatatt tattatatat
    15961 taatggggga tatttcttta attaatagaa ctatattagt attacagtta atattataat
    16021 acataattgg tcattttaat tcattttata ttaaaatttt taataatatt atttcaatgt
    16081 gaatataaaa ttgcagaact aattcaaaat aatttttacg actacaaata acctataata
    16141 tacttttaaa gaaagtatcc taacaagaat atataaaaat ttaaaagggt tgatttaaaa
    16201 ataaccatca acccttttta ctaatatcta tttatattac catcaaatct tacccaaata
    16261 ttttttcttg caattgtctt aaatgatata aagtagtatc tttttctatc tctaccatat
    16321 cgtaagttat aacttcacct tttttaatat cttttacaac tttagtcttt ttacttatca
    16381 aaccaatagg tacagcattc atagcctttg ctacatcgta tttttctata gtaccatagc
    16441 atgtatatgc tccatagcca tccataaact cacctttttt caaatctttc ttagctatag
    16501 tcataacttc tgctactagt ccagctttag gtactattgt agccttatgg tctaagactg
    16561 ccatagctgc tgataaagga gtctctaaac tacataaatg atatggtcta tataatatgt
    16621 aattaggtcc atcacccata cttaaatatt ttagttcatc attaactgct tttaatttat
    16681 gggctactac tacgaaaact ccaggtgcta taccatttac aaaatcaact acaccataat
    16741 tatctaatac tccaccttcg ctttttaatg catatttctt aggtacttca ttaactgtag
    16801 cttcaatacc atgaccacct cttacatcag gcacaaatcc agttgcatta cacattaaag
    16861 ccatttctac cattgtttta gttccttctt taaaagaagc tatcatatga ggtgaagctc
    16921 cttttctttc tgcttcttct ctaactgtat caggattaca atctaaatct aatttattat
    16981 ttttaccttt acctatcact ctaacttcaa accccattgc atctgcaaaa tcaaataatt
    17041 ccattactgc acctggctca tctcctgcag aacctgtaaa tattactcca gcatcatctg
    17101 ctaatttttt aagtattggt ccaacaacac aatcagtttc agcatttaac ataactatat
    17161 gttttttatt taaaatagag tttaatgcta tctctgcacc aattgcaata cctccagttg
    17221 catctataac tacatctatt gacttagttt tacaagccac aagaaaatca ttacatgtca
    17281 atacttttcc ttcttgtaat tttatttctg catccttttc atttgttaca ttatctataa
    17341 tttcttcatc tattcctgca tcagtaaaag ctttatgagc caactccaaa tttatatcta
    17401 caacaataga agcttcaata cctttcatat ttgccatttg gcttaccatt ccatttccca
    17461 tatgcccagc tcctataaga gctactttta tttttttacc ttcctcttcc aattttgcaa
    17521 gttttctgtt taaatctaac ataccaattc ctcctgttat aataatttta ttttcaaatt
    17581 aagaaatagt tattaaatct ccaatagtta tatctggatt tcctatttcc acatgtattt
    17641 ctcctggtaa atttacttca cattttccat caaatttgat tgtacaatgc ccaatttctc
    17701 taagagtctt ctgagcaata tcaccaactg aaactataca ataatctcta ttccctatag
    17761 taagtgtatc tcctatttca acatcacttt ttaacgttgc tattgaatga attacagata
    17821 tatcctttaa gtcgtcatca ttaatggttt catcatatat aataagacaa ttgctttcat
    17881 ccattaactc tttaactaaa ctgcctatcc cacttatctt aacttcatat ttcatgacat
    17941 tcctcctgat aatttatatt tatattgagt taattaaaag aaacatttat tttttctttt
    18001 ataaatttta tagcttgtac aatactctct tcatctttaa atttttgctc tgcactatat
    18061 atatctaatc cttctatttc ttcaatctct ttaaatttag ccattactgt acgtcctttt
    18121 aaaatttctc caaactttat aaacccatct gaatctgaag atataatggc tatacttcct
    18181 ttagaaaaat agcttttctt tttgcctgta gacactatat cacccttttt tttagctcta
    18241 tttaaagttt taaaatacaa tttattctgc ttcattataa atatagtatt taaaatataa
    18301 gcaacaaata aaatcactaa taatttcata tttatacacc ttccaaaaat cacattattt
    18361 ttaaatctgt ataacaacta taaatgctgt acaatagtta tataaggttt ttaagtaaca
    18421 cctttttaaa ttctaaaaaa gttctacatt tacttatctt attcgttaat tttacatctt
    18481 ggcctatctt taaaacttct tcataaattt ttactaataa gtctgagtct atcttattct
    18541 cacatggaaa cattgtcata agaattattt ttatacttct atttgcatat tctattggtt
    18601 cttctaaaat tccaactgcc attaaaaact tatctgattt ttcatttaca gaatgaggaa
    18661 ataataaacc tttatcaaat actgtgggag atttttcttc tctattgact atattttttc
    18721 taaacttatc atcaatgcat cctaaatcca taagatggtc catcattttt tctaatgaag
    18781 caagtatagt cttttcattt aatatcataa atttatcttc atctaacaaa ttatttatta
    18841 gtaaatttgt agaattagta gtgtcaatat tcccttctct taggcacaat actgttttta
    18901 attgttctct tagtttgttt tcatcaaata ttgtatttat ttttaatact attgtattta
    18961 tatttgatgt gtctaaatca atagttgtaa aaactatatc atattcatct aagttaatat
    19021 ttctcaaatt aaaacttgaa agagtatcaa ttttaggatt atttcctatc agtttttcta
    19081 ctcttatttt caatagtttt gctgttccta gacctgtact acatataatt gctatttttt
    19141 ttattgaata gaatctactg ctatttcttt ctaaatatcc actgaaatgg attgctatat
    19201 atccaatctc atcttctgaa acttctaagt tatacttttt ctctattact tttcctgcta
    19261 tttgtgctaa tttaaatgga agtatataat tttgcttaat ctcttctaac aaaacatttt
    19321 ttaactttat attaaataat agcctgttta aagtaaaact aagatgatat cctagaccta
    19381 ctcttaattc atcctcattt ataaaaatac ccatatcgac atatatttga ttaaatatat
    19441 tttccatcaa ctcatcaatg tttttactaa ttctactact atttaaagca cattctgatg
    19501 ctggtgcatt tccactaact aacggtatag ttaaataaat aatttcttca tcatttaaag
    19561 tacattcaaa cattcctgat attatatgtt ttatttccct agcaataaag tattcatcat
    19621 atgatttaag taaatcaaac t
//
LOCUS       DACULQ010000061        19590 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.061, whole genome shotgun sequence.
ACCESSION   DACULQ010000061 DACULQ010000000
VERSION     DACULQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.061"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            64..399
                     /locus_tag="JA154_08770"
     CDS             64..399
                     /locus_tag="JA154_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995959.1"
                     /translation="MIKLWKKEDLNLLLEYPKEVVENVDNVINILDENYGCNRKLTDD
                     GGYVCIIEDIKEVENLKSNILKGLVEEFSDVIYEDEVNTYNSTLYLLSSDYSVTVISK
                     NEETEYLLK"
     gene            463..921
                     /locus_tag="JA154_08775"
                     /pseudo
     CDS             463..921
                     /locus_tag="JA154_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            1061..1354
                     /locus_tag="JA154_08780"
     CDS             1061..1354
                     /locus_tag="JA154_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995960.1"
                     /translation="MFKKENRYMTKEIAENLPIEIAILLWNLIDDLTTEKDYLQLFEI
                     NPIGLGVVEIVHKQEVPEYEARIYIQNDKIKDKLKIYVIDNVEYRTMMFSNEY"
     gene            1381..1977
                     /locus_tag="JA154_08785"
     CDS             1381..1977
                     /locus_tag="JA154_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995961.1"
                     /translation="MINNKSEIINKLFFNELKELIDKYNNIDDKTVDVIEKIFTCIED
                     EEIKNYLIRDNCKLQELVNTYNKLEDLNIDEAIFFAWYNLTINTISIDKASEYYDDLT
                     TSNYIEVENHIIYTNERDLRKYAEDELETMLSMEHHIDRLLHKDMLIDMWLNNTSKEE
                     VIEEILMSDDIEDILNLSPEYAFTLANGIEYRYSEKEE"
     gene            2178..2399
                     /locus_tag="JA154_08790"
     CDS             2178..2399
                     /locus_tag="JA154_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HAT7995962.1"
                     /translation="MVEVLGEEIVIKLHKYYRGQQITFPMKLYSIEYVERYIVKNYRT
                     KTIKEMCRELGYTEGWIKQLINKNRLNSK"
     gene            2425..2667
                     /locus_tag="JA154_08795"
     CDS             2425..2667
                     /locus_tag="JA154_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995963.1"
                     /translation="MILTVLAILMGTGIFFLINSMLDITYFGCGPIGFLWFGCFIVSY
                     AILTSLGGIVLGLLKWIVIGLIILFVIGLIANKIND"
     gene            2873..4006
                     /locus_tag="JA154_08800"
     CDS             2873..4006
                     /locus_tag="JA154_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAT7995964.1"
                     /translation="MQDFDFISETIKKFQSDLENMDKVNILVVGKTGVGKSTLINNIF
                     REELAETGIGRPVTQHLKKISKEGLPINLYDTKGLELEEDVQETIKKEILDEIENCNR
                     NSLERNDKSELMHVCWYCINTSVARCEETDIKWINELSEKIPVIVVLTQGFPRKKARE
                     LKSSIDDLNLNCKNVIPVLAEQLVEEEDEIVIKSYGLDKLVEVTYQVIPKEVKKAFVN
                     VQKVDIKKKVEESRKWVLGYVGGSGAIGFSPIPFSDAPLLATAQVAMISHITVIFGLQ
                     VDKALLTAIVSSIGGVTSATLLGKTLVSNILKFIPGAGTIAGGAISGSVAALTTTALG
                     MAYISVLEYILNENMKGNEVNNTEIAEKMKDAYKDQLGMLSKK"
     gene            4367..4635
                     /locus_tag="JA154_08805"
                     /pseudo
     CDS             4367..4635
                     /locus_tag="JA154_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427580.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4770..5015
                     /locus_tag="JA154_08810"
     CDS             4770..5015
                     /locus_tag="JA154_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995965.1"
                     /translation="MRSVTVEDGDERFAIEKIYIKSLQRYEVRICLYRDCRDRINKLI
                     PRPVDLTMDKFVALILVGLKAGVLDDDFKQELIKGLA"
     gene            5363..6724
                     /locus_tag="JA154_08815"
     CDS             5363..6724
                     /locus_tag="JA154_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995966.1"
                     /translation="MKGNINKKDIDLSKITFDKFLEDEGLQNRERDYLYVDDYDNTLD
                     TQEFKLHIIEENTDNTYIDKDKYWYLDLDDSLFEYEEDEDTIAKTLYSLIDIRIKFLK
                     HVEYRKYINLGVIDEEYFDEIKSFLKFYIVAKTKKNELGNSNLKQMAIDIFDKERFED
                     DISDDDMENISEWIGEDQNFKMFYDKVCRDLSNSTQDKEILKLAGIRNNKVKHYKGRK
                     ISRKCKLELKSLTKMWEYETKLTHDANKAHKKIKSNMLEKMTDKNYKNIYEYLNNEII
                     NSNEEYKNIYFYSLELKLANELRKCTLKNLNKIKDIENQVKFISYTIAYIHIVPVLTI
                     REKLSDKLFNILSTNEGKDIEKFTKEVSLIMRIINIECLELLNIVSKNNYYKNFSEKE
                     LEEFKSIYNINNGRKSFENKYKIKVDYNIDDVRKYGEVIKAINIESKKKIVDRIKKKR
                     KSN"
     gene            6914..7231
                     /locus_tag="JA154_08820"
     CDS             6914..7231
                     /locus_tag="JA154_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995967.1"
                     /translation="MTNFKSVKVELILLIECEEGNRNELEGMIDEGVLNEKEYSLTIL
                     GSTEYEDNARAIYILMNTGGSYEKNLQRLSRLHLKIENLLKDTNVKYRGISLVPNIVK
                     WDK"
     gene            7243..9006
                     /locus_tag="JA154_08825"
     CDS             7243..9006
                     /locus_tag="JA154_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995968.1"
                     /translation="MSKAINREELIKLYEEIGLNVLDNNTVMNSSNKKICNFLVNIIE
                     QRVIDGKMYLCINAMGINGELLSEVILDSNTINRTTWVIENWGFKYSIDKKYINDFIE
                     LIQHLCMNIKTKYIYDHTGWIYQDNKWIYLHGGGVIGGSDVQVELDESIDNYILPDKI
                     SDINKACEMSLKLVDLIEDDKGIIFSSLVYLSPLIEIISRKIKPPENIVWTYGKTGSR
                     KTAVSKVVLSHFGDFNKKIPATFNDTITAIELKANKLKDSLYLCDDFAPKQDFRDKKI
                     QDSKAESIIRMYGDRVGKGRSSNKLDIKDAKRPRGMILITGEDVICGESSNARLLSIE
                     LNRSSVDLNILTELQNNSHLLGEAMRGYIEWLLSEMGNIDELSNILLYNFKSYRDEIR
                     EQYKDDIHGRTIESSAWILVGFSCMLNYIRDKGVIDKYEDDEYMDRAIKVVERLIEEQ
                     IKLISRNSPIDIFLNTLKESINSKSIRIATLNDQNQVIEDKLEYICGYKDKEYYYFYT
                     DKVYSFIEFENKKRGIHLGISQRQLVKQLRDSGIVKVDSNNDSPKKTIHTKDASDSTG
                     YTTIRPRMLQIERSIIENFQI"
     gene            9311..10876
                     /locus_tag="JA154_08830"
     CDS             9311..10876
                     /locus_tag="JA154_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAT7995969.1"
                     /translation="MTGKKAVVYARFSSDNQRDESIDAQLRAINEYADKNNIKIVNQF
                     IDRAKSATSDKRPEFQNMIKFCEADTTGISMVIVHKLDRFSRDKYDSAMYKQKLKVKG
                     IRVVSVLENLDNSPESLILESVIEGMAQYYSANLAREVAKGQKENALKALHNGGDAPL
                     GYDVAHDKTYTVNEEEAQAVKIIFDRYIKGYSYSKIIDELNILGYKTKRGNKFGKNSL
                     HGILNNEKYTGVYVFNKTQRKGVDGKRNGHKQKSEEEIIKIEGGMPQIIDKGVFKQAQ
                     DMIQKRKKTPGAHKATTTYLLTGLIKCGECGHAFQGNKRKDSYGNEYVSYRCGCRKQK
                     RECNNREIKRDYLEEFILQELEKNILNDEAIPILSKALNEKLNDKNESNAELLKNLEQ
                     KLDKVNKEISNILNAIMNGIVNSMLKDKLDELEQVKFNLDLKMNELKIENKVVDGVGV
                     TEDQIRGMFSRFKEFVLERNIPECKKFIGDYIKEVIVYKDHIEVIFNVVFCFVDNDIT
                     HDLHIITETGKIT"
     gene            11022..12314
                     /locus_tag="JA154_08835"
     CDS             11022..12314
                     /locus_tag="JA154_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995970.1"
                     /translation="MADIFTVLNYQGSKKRLLDFIYENTQEYIDSDKAFLDICSGTSA
                     VAYNMKRHFTVYTNDSENYAYQISNSIVKNNERYNFSQSDEMIIREKYNINLQRLNNI
                     FNDMVNQEDMLLEQEDADGLIELYSNCPCIWKNNVDIEIEGNTIQSIYDLRKCKRDIP
                     NVLFTLYYSSSYFGIKQSMEIDSIRYAIEFIESEITKSILFTSLFFAMKECVFAKDGH
                     MAQPLNMETGKKRMLRLKARSIYDIFIDKLKEFNTEEFVLSQKENKCFNKDFRTLLET
                     EESIKDEVGFIYIDPPYTDMQYSRYYHILNTVVLYDYPEISLSRGTISKGIYREGRYQ
                     SPLSQRKHALNEIDFLFSFCKENKINVALSYAYPIDTEKQATNRYTASIDDLINVAKK
                     HFGDNVKVVHEEYSHANNRNSDKKNVLEYLIMGIHNKE"
     gene            12317..13936
                     /locus_tag="JA154_08840"
     CDS             12317..13936
                     /locus_tag="JA154_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995971.1"
                     /translation="MDKINKLKERIANIEATNKNDIYSSNLYWSQKPFNICDEIIEHL
                     SEDGDIIFDPFMGSGVTVIEALKKEYKRNAIGCDINDAPIFIVKTLLEKYNVPKIEKD
                     IKELNNKLSKLSTYYSTKCRECGEVVTISKTIFDKENRNAKCELKSIHYKCKECKNDF
                     KGEPNETEISKFFIDEELKNIDDFQFIANSRLAVGEGDTVRTIFSNRNLIVLDKILGH
                     IDEYKNSESYEPMKYILLSILHLSKITDTHSNSQWPLWIPKKDCVEKNVVELFIKNSK
                     KTIKALKLASKYLCENRENVELIKNLDRGKFKLIKDGVQNIEDDVIPNEFVDLIITDP
                     PYMGQVLYSEYMQLYKPFMDLEFELENEIVISSAPARDKGKVLYFDMMNSAFENISRV
                     LKVGKYMCMYFHDSNLDVWDNIIDIMTNNNLKYMGQIHVSKNKNTLKNILSPKKSLNG
                     DCIIFFKKDLNINYDINEVDNIEDKINNIAQAIIDIKGYASTPELYDNGALEYIISNG
                     KLKEISKKYKDLTSIFEQRFNWDKERGVWTV"
     gene            complement(14061..15212)
                     /locus_tag="JA154_08845"
     CDS             complement(14061..15212)
                     /locus_tag="JA154_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995972.1"
                     /translation="MKKLTIRLKDDFHKDLLDLVAFKNDSINSYVINLIKNDLSKNTN
                     LLRSYDNMIRDNITNVLITQKINELKAIQDGIFKEYKVNDILSNSKIYEVMTANSLNH
                     ILIPGHSGSRDAKDEFGNEFEYKHFKKSSSNHSWTFNDFSDTSIAKLKKAKAVIFAHI
                     NDVDFPYPGKLDWYYSIPGDIMCEYLLKYTQSIQNSRKMINVSPNQLESRLNASKIIP
                     SAQNGVYDKYLSAIYKASSELEELTGVKNLLTSNKFWELIVALELNHQVNPEQGGREG
                     AHDAYDSEGNPYEYKVSKNHAWNFQDISDNVLNKYYNDKAIILAVVDKENIEVEHIYS
                     TEPADVVPLLRKKLQEKINRCLEKGSPIRRLQVSLSKSDLTRVNAKKLY"
     gene            15702..15917
                     /locus_tag="JA154_08850"
     CDS             15702..15917
                     /locus_tag="JA154_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7995973.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            15917..16396
                     /locus_tag="JA154_08855"
     CDS             15917..16396
                     /locus_tag="JA154_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HAT7995974.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFDSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            16396..16632
                     /locus_tag="JA154_08860"
     CDS             16396..16632
                     /locus_tag="JA154_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995975.1"
                     /translation="MIMEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKL
                     ISEGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(16748..17914)
                     /locus_tag="JA154_08865"
     CDS             complement(16748..17914)
                     /locus_tag="JA154_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT7995976.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPLRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKYLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQGPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYINNNYVFCNELGIPIDDKRPLRNLKSILNSLNIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(17904..18656)
                     /locus_tag="JA154_08870"
     CDS             complement(17904..18656)
                     /locus_tag="JA154_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995977.1"
                     /translation="MNKSYIPKYTHKFPTNIPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILINNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(18748..19239)
                     /locus_tag="JA154_08875"
     CDS             complement(18748..19239)
                     /locus_tag="JA154_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7995978.1"
                     /translation="MMNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDI
                     KTDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(19236..19434)
                     /locus_tag="JA154_08880"
                     /pseudo
     CDS             complement(19236..19434)
                     /locus_tag="JA154_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19491..>19590)
                     /locus_tag="JA154_08885"
     CDS             complement(19491..>19590)
                     /locus_tag="JA154_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7995979.1"
                     /translation="YFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
BASE COUNT         7964 a         1932 c         3202 g         6492 t
ORIGIN      
        1 agatgttggt agaaatacga acatctcttt tttagtgcaa aaaattaaaa ggaggaagtt
       61 aatatgatta aattatggaa gaaagaagat ttaaatttat tattagaata tccaaaggaa
      121 gtagttgaaa atgttgataa cgtcattaat attcttgatg aaaactatgg atgtaacaga
      181 aaattaacag atgatggtgg atatgtgtgt ataattgagg atattaaaga agtagaaaac
      241 ttaaaatcaa atatattaaa ggggttagta gaagagttta gtgatgtaat atatgaagat
      301 gaagttaata catataattc aacactatac ttgttatcta gtgattatag tgttactgta
      361 atttctaaaa atgaagaaac tgagtactta cttaaataag catatacatt gataaggtgt
      421 atatgcttat tttaatttta ataaataaag gagaaaagca gaatgaataa aaagagaact
      481 cctgccaaga gagttgatat agttttactt aaattagtaa aagaacgtag tgttctatat
      541 gaaactagaa aaataagtaa tctttatgat gcttatagat tagcaaagaa ttttttataa
      601 atagtgctag agaaaagttt gtagtggtat gtttagatac aaagaattgg ccagtagcat
      661 agaggttgta agtataggca ctgtaaattt atctatggta tatccaagag aaatatttaa
      721 agtagctata ttaaccaatt catctgaaat gatatgcttt cacaatcacc caatgggtaa
      781 tactgacttt agcaaggaag atagttataa ctaatatgct acagaagcgt ggagaaatat
      841 tatgcattga attagtagat acatatagtt attgtagatg aggataaata ctttagcttt
      901 aaagaaaact gtaggatata agagtcttag cttaaataaa ttaggctaag gctttttcta
      961 tgttccaaaa caattaaaaa taaatcaaat ttaggttaag cccggaaagc ccttgaaaaa
     1021 aataatacat ataaatttaa aactaaatag gagttgtaac atgtttaaaa aagaaaatag
     1081 gtatatgaca aaagagatag ctgaaaatct accaattgaa atagctatac tactatggaa
     1141 tttaatagat gacttaacta cagaaaaaga ttatctacaa ctatttgaaa taaatcctat
     1201 aggattagga gtagttgaga tagttcataa gcaagaagta cctgaatatg aagctaggat
     1261 atatatacag aatgataaaa ttaaagataa attaaaaata tatgttatag ataatgttga
     1321 atatagaaca atgatgtttt caaatgaata ttagttaatg attaaaggag agagagtaat
     1381 atgataaata ataaatctga aataattaat aagttatttt tcaatgaatt aaaagagtta
     1441 atagataaat acaacaatat agatgataaa actgtagatg taatagaaaa gatatttaca
     1501 tgtatagaag atgaagaaat aaagaattac ctaataagag ataattgtaa gctacaagag
     1561 ctagtaaata catacaataa acttgaagat ttaaacatag atgaagctat attctttgct
     1621 tggtacaact taactataaa tacaatatct atagataaag caagtgagta ctacgatgat
     1681 ttaactacaa gtaactatat agaagtagaa aatcatataa tatatacaaa tgaaagagat
     1741 ttaaggaagt atgctgaaga tgaattagaa actatgttaa gcatggaaca tcatatagat
     1801 agattactac ataaagacat gttaatagat atgtggttaa acaatacttc aaaagaagaa
     1861 gtaatagaag aaatattaat gagtgatgat atagaagata tattgaattt atctccagaa
     1921 tatgcattta cattagctaa tggaatagaa tatagatact cagaaaaaga agaataaaat
     1981 aagtatatgt actgaaaaat acgggcttta agggcttata cattaaagta catatttgaa
     2041 aataccatag attttacaag agaaaatcag aatgaaactt tttcaaatac tagttattat
     2101 taattagtgc tagaaaagga ggtggagtga tgactaacaa caataataat tgtgattata
     2161 agggcatata tgcagatatg gttgaggttt tgggtgaaga aatagtcatt aaattacata
     2221 agtattatcg tggacaacaa ataacatttc caatgaaact atattctatt gaatatgtgg
     2281 aacgatatat agtgaagaat tatagaacaa aaacaataaa agaaatgtgt agagagttag
     2341 gatatacaga gggatggata aagcaattaa ttaataaaaa tagattaaat tcaaagtaat
     2401 tagagaagga gattgacagt agatatgata ttaacagtat tagcaatact tatggggaca
     2461 ggtatatttt ttctgattaa ttcaatgcta gatataacgt attttggatg tggcccaata
     2521 ggatttttat ggtttggatg ttttattgtg tcatatgcaa tattaacatc attaggtggt
     2581 atagttttag gattgttaaa gtggatagta ataggattga ttatactatt tgtaatagga
     2641 ttaatagcga ataagataaa tgattaaatt actgtagatt attttgataa aataattatt
     2701 taatatattg aggcattaca tgatagaaga ataaatataa atgacttaaa atctttaact
     2761 taactaaaca aaaaatatat tatttattac aaaaaatgac aaaaaaatta aatattatat
     2821 tgtattatga caatataaaa cattggagtt tataaattag gaggaatatt ttatgcaaga
     2881 ttttgatttt ataagtgaga caataaagaa atttcaatcc gatttagaaa acatggataa
     2941 ggtaaatata cttgttgtag gtaaaacagg agtaggtaaa agtacattaa taaataatat
     3001 atttagagaa gaattagcag aaactggaat tggtagacct gtaacacaac acttgaaaaa
     3061 aataagcaaa gaaggattac caataaattt atacgataca aagggattag agttagaaga
     3121 agatgtgcaa gagactataa aaaaagagat attagatgaa atagaaaatt gtaatagaaa
     3181 ttcattagaa agaaatgata aaagtgagtt aatgcatgta tgttggtatt gtataaatac
     3241 atcagtagct agatgtgaag aaactgatat aaaatggata aatgagttaa gtgaaaagat
     3301 accagtaata gttgtattga cacaaggatt cccaagaaaa aaagcaagag aattaaaatc
     3361 tagtattgat gatttaaatt taaactgtaa gaatgtaatt ccagttttgg cagagcaact
     3421 agtagaagaa gaggatgaga tagttataaa gtcatatggt ttagataaat tagtagaagt
     3481 tacatatcaa gtaataccaa aagaggtcaa gaaagctttt gttaatgtac aaaaggtaga
     3541 tataaaaaaa aaggttgaag agtcgagaaa atgggtttta ggatatgtag gaggatcagg
     3601 agcaatagga ttttcaccaa taccattttc agatgctcca ttattagcaa ccgcacaagt
     3661 tgctatgata tctcatataa cagtaatatt tgggttacaa gttgataaag ctttactaac
     3721 ggcaattgta agttctatag gaggggttac atcagctact ttactaggaa aaacattggt
     3781 atcgaatata cttaagttta taccaggagc tggaactata gcaggtggag cgataagtgg
     3841 ttctgtagcg gcattaacaa ctacagcatt aggaatggca tatataagtg tattagaata
     3901 tatactaaat gagaatatga aaggtaatga ggttaataat actgaaatag ctgaaaaaat
     3961 gaaagatgca tacaaagatc aattaggaat gttatctaag aaataattaa ttcaagataa
     4021 agtataagat taattatagg gaaaaagtaa aatttaaagg tatatttggt aaaacataaa
     4081 caaaaatgat aatggcgtca atattatttg ttgtcatatg tagtagctta gtatatatat
     4141 tattgagtga taggtttaat cataagttaa tttacttaga gaaagatata ttagatttaa
     4201 gaaataaaaa tttgtaacta gctaataaag gatcagtata gaaacagata aacaagagga
     4261 taatgtagat attaaatcta aaaacactaa taatgaagaa tataaaactt caaaactatt
     4321 taggcactag atagagtatt tgctataatg aataagctaa tagagaaatt aaaacgttta
     4381 tagtaagata actaattcaa atacatttag tggacttcaa ggaacatttg atacactatc
     4441 aaactatggt agctaattga tagaccaatc atctaaattg aatttatcaa atgttggatg
     4501 gttagaaata tctgatataa ttacaagtat aggaaaggac tattagattc tgaaaatatg
     4561 tcatttgaag cattaaatat aatgggaaaa ataggaaaat ttataaatta tattgaaatt
     4621 aattatatta actaattaaa tattgataca taatgccttg ctacagttaa atgtggcaag
     4681 gcattatttt tgtaaagaaa ggaagaatta acatggcgaa gcaaagtaaa gaggaactat
     4741 ttgaaaaaga aactaaatat tgcaagataa taagaagtgt aacagtagaa gatggagatg
     4801 aaagatttgc aatagagaaa atatatatta agtcattaca aagatacgag gttagaatat
     4861 gcctgtatcg tgactgccga gatagaatta ataaacttat tccaaggcct gtagatttaa
     4921 ctatggataa gtttgtagca ttaatactgg taggattaaa ggctggtgta ctagatgatg
     4981 atttcaagca agaactaatt aaaggtttag cttaggaggt agaatatgac aatagaagag
     5041 tttactagtg gagtaatgtt tgggatttca atttttagtg cagtattaaa gcaagtatta
     5101 gaaaataaaa aatagtatac aggaggtatg agtatgattg gatttataat aggaagtata
     5161 ataggaattg gactatgtat tagtatagag atactgatat cgaaaaatgc cataatatac
     5221 taagttatat ttaaattatt aatagattca tatataattt atttaagccc ttaaagccca
     5281 gataagttat attaaagtaa agtttgtatt tatgtaaatt aaataatata tattattata
     5341 aatttgaaag gactttataa atatgaaagg caatataaac aaaaaagata tagatttaag
     5401 taaaataaca tttgataagt ttttagaaga tgaaggatta caaaatagag agagagatta
     5461 cttatatgta gatgattatg acaatacttt agatactcaa gaatttaaat tacatataat
     5521 tgaagagaat actgataaca catatataga taaagataaa tactggtatt tagatttaga
     5581 tgatagttta tttgaatacg aagaagatga agatactatt gctaaaacat tatatagtct
     5641 aatagatatt agaattaaat ttttgaaaca tgttgagtat agaaaatata taaatctagg
     5701 agtaatagac gaagaatatt ttgatgagat taaatcattt ttgaaatttt atatagtagc
     5761 aaaaacaaag aaaaatgagc taggtaattc aaatttaaag cagatggcta tagatatttt
     5821 tgataaagaa aggtttgaag acgacatatc agatgatgat atggaaaata taagtgaatg
     5881 gataggagaa gatcaaaact ttaaaatgtt ttatgataaa gtttgtaggg atctctcaaa
     5941 ttctacacag gataaagaaa tattaaaatt agcaggaatc aggaataata aagtaaaaca
     6001 ttacaaagga agaaaaatta gtagaaaatg taaattagaa cttaaatcat tgactaaaat
     6061 gtgggagtat gaaactaaac tgactcatga tgcaaataag gcacataaga aaataaaatc
     6121 taatatgctt gaaaaaatga cagataagaa ttataaaaat atatatgaat atttaaataa
     6181 tgagattata aatagtaatg aagaatataa aaatatttat ttttatagct tagaattaaa
     6241 gttagcgaat gaattaagaa agtgtactct taaaaactta aataaaatta aagacataga
     6301 aaatcaagta aaatttattt cttatactat tgcttatata cacatagttc cagttttaac
     6361 gataagagag aaactaagcg ataagttatt taatatattg tcaactaatg aaggtaagga
     6421 tatagaaaaa tttactaaag aagttagtct tataatgaga attataaata tagaatgttt
     6481 ggagttatta aatatagtta gtaagaacaa ctattataaa aattttagtg aaaaggaatt
     6541 agaggagttt aaatctattt ataatataaa taatggaaga aagtcatttg aaaataaata
     6601 taaaataaaa gtagattata atatagatga tgtaagaaag tatggagagg ttataaaagc
     6661 aataaatata gaaagtaaga agaaaattgt agatagaatt aagaagaaga gaaaatctaa
     6721 ttagattttc tctatttttt ttgaaaaaaa taaaaaatat ttggtgaaaa cgtttatctt
     6781 taggtgatat gagtagtcat tatttattat ataatcatat ttgtaggaac gagctaatta
     6841 ataaaatcta agaacaatag cttaatgatg aaaaagtacg gcctttcggg ctttgacaac
     6901 aggaggtata ttaatgacaa actttaaaag tgtaaaggta gaattaattc tacttattga
     6961 atgtgaagaa ggaaatcgta atgaattaga aggtatgatt gatgaaggtg tattaaatga
     7021 aaaagaatat agtttaacta tattaggtag tacagagtat gaagataacg caagggctat
     7081 ttacatactt atgaatacag gaggatcata cgaaaaaaac ttacaacgat taagtagatt
     7141 acacttgaaa attgaaaact tacttaaaga tacaaatgtt aaatatagag gtatatcttt
     7201 agtaccgaat attgttaaat gggataaata ggaggaatac aaatgagcaa ggcaataaac
     7261 agagaagaat taattaaatt atatgaggaa ataggattaa atgttttaga taataacacc
     7321 gttatgaatt catctaataa aaagatatgt aattttttag taaatataat agaacaaagg
     7381 gttatagatg gaaaaatgta tttatgtata aatgctatgg ggattaatgg agagctatta
     7441 tcagaggtaa tattagattc gaataccata aatagaacta catgggtgat tgaaaattgg
     7501 ggttttaaat attctataga taaaaaatat attaatgact ttattgaatt gatacaacac
     7561 ttatgtatga atataaaaac taagtatata tatgaccata caggatggat atatcaagac
     7621 aataagtgga tatatctaca tggaggagga gttataggag gttcagatgt tcaagttgag
     7681 ttagatgaat caatagataa ctatatacta ccagataaaa ttagtgacat aaataaagca
     7741 tgtgaaatga gtttaaagct agtagactta atagaagatg ataaaggtat tatattctct
     7801 tcattagttt acctatctcc acttattgaa attatcagta gaaaaattaa accaccggaa
     7861 aatatagtat ggacatatgg aaaaacagga tcaagaaaaa cagcagtatc aaaagttgta
     7921 ttaagtcatt ttggtgattt taataaaaaa attccagcta catttaatga tacgattaca
     7981 gctattgaat taaaagctaa taagttaaag gatagcttat acttatgtga tgactttgca
     8041 cctaaacaag actttaggga taaaaaaatt caagactcaa aggcagaatc cattataaga
     8101 atgtatggag atagagtagg gaaaggacgc tcaagtaata agttagatat aaaggatgct
     8161 aagagaccta gaggaatgat attaataaca ggtgaagatg tgatatgtgg agaatcatct
     8221 aatgctagat tattatcaat agaattaaat agaagttcag tagatttaaa tatactaact
     8281 gaattacaga ataatagtca tttgcttgga gaagcaatga gaggatatat agaatggtta
     8341 ttatctgaaa tgggaaatat tgatgaatta tctaatatat tattatataa ctttaaatct
     8401 tacagggatg aaattagaga acaatataaa gatgatatac atggaagaac tattgagtct
     8461 agtgcatgga tattagtagg ttttagttgt atgttaaatt atattagaga taaaggtgtt
     8521 atagataagt atgaagatga tgagtacatg gatagagcga ttaaagttgt agaaagatta
     8581 atagaagagc agataaaatt aatatcgaga aactcaccta tagatatatt tttaaataca
     8641 ttaaaggaat caataaattc aaagtcaata agaatagcaa cattaaatga ccagaaccaa
     8701 gttatagaag ataaattaga atatatatgt ggatataagg ataaggaata ttattacttt
     8761 tacacagata aagtatattc ttttatagaa tttgaaaata aaaaaagagg tatacatttg
     8821 ggaataagtc aaagacaact agtgaaacag ttaagagata gtggaattgt gaaagttgat
     8881 tctaacaatg attctcctaa aaaaactatt catacaaaag atgctagcga ttcaacagga
     8941 tatacaacaa taagaccaag aatgcttcag atagaaagaa gtataattga gaactttcaa
     9001 atataactta taatcaagag tgcttatgta ctcttgatta tattctatga taaatggagg
     9061 atataaatat gacagaaact tataaaatca gcataggaag aaaagattta tattctaaag
     9121 actataaggt agttgaaaga actgactatg aaaagagaca cacagttaat ataagtagaa
     9181 gcttagtaga catactttct atacttgtaa aagaagatag atagattaaa atatggttac
     9241 acacttagtg ttagtgtgta gcctttaata tagattaaaa ttagtatatt taaactttgg
     9301 aggaaataat atgacaggaa agaaagcagt agtatatgca cgctttagtt cagataatca
     9361 aagggacgaa tcaatagatg ctcaattaag agcaataaat gaatatgcag ataagaataa
     9421 tataaaaata gttaatcaat ttatagacag agcaaaatca gcaacatcag ataaaaggcc
     9481 agagtttcaa aatatgatta aattctgtga agcagataca acaggaatat ctatggtaat
     9541 agttcataag ctagatagat tttctagaga taagtatgat tcagctatgt acaaacagaa
     9601 attaaaagta aaaggaataa gagttgtaag tgtattagag aacttagata attcgcctga
     9661 atccctaata ttagaatcag ttatagaggg aatggcacaa tattacagtg ctaatttagc
     9721 gagggaagtt gctaaaggtc agaaagaaaa tgcccttaaa gcccttcata atggaggcga
     9781 tgcaccatta ggttatgatg tagcacatga taaaacatat acagttaatg aagaagaagc
     9841 tcaagcagta aagattatat ttgatagata tattaaagga tattcatata gtaagataat
     9901 agatgaacta aatatcttag gatataagac taaaagaggt aataaatttg gtaagaatag
     9961 cttacatgga atattaaata atgagaaata tacaggagta tatgtattta ataagactca
    10021 gagaaaaggt gtagatggaa aaagaaatgg acataagcaa aagagtgaag aggaaataat
    10081 aaaaatagaa ggtggtatgc cacaaataat agacaaagga gtatttaaac aagcacaaga
    10141 tatgattcaa aaaagaaaga aaactccagg tgcacataaa gctactacta catatctatt
    10201 aacaggacta attaaatgtg gtgaatgtgg acatgcattt caggggaata agagaaaaga
    10261 ttcttatgga aatgaatatg tttcatatag atgtggatgt agaaagcaaa agagagaatg
    10321 taataataga gagataaaaa gagactattt agaagagttt atattacaag aacttgaaaa
    10381 gaacatatta aatgatgaag ctataccgat tttaagtaaa gcattgaatg agaagctgaa
    10441 tgataagaat gaaagtaatg cagagctact taaaaatcta gagcagaaac tagataaggt
    10501 aaataaagag ataagtaata tactaaatgc tataatgaat ggtatagtaa atagcatgtt
    10561 aaaagataag cttgatgagc tagaacaagt aaagttcaac ttagatttaa aaatgaatga
    10621 actaaagata gaaaataaag ttgtagatgg tgtaggtgta acagaagatc aaataagagg
    10681 tatgttttca agatttaagg aatttgtact agagagaaat atacctgaat gtaagaagtt
    10741 tataggagac tatataaagg aagtaatcgt atataaagac cacattgaag tcatattcaa
    10801 tgtggtcttt tgttttgttg acaatgatat aactcatgat ttacatatta tcacagaaac
    10861 gggtaaaatt acatagtaaa tatatatttt ataaaagtaa atatcagata ttatttagat
    10921 aaaaattatt tttgaaaata ttagttttaa aaaatatatt tatataatta taaaataaaa
    10981 tggtataata aaactttaaa tatgataaag aggtgagact attggcagat atatttacag
    11041 tactaaatta tcaaggtagt aaaaagagat tattagattt tatatatgaa aatacacaag
    11101 aatatattga ttctgataaa gcttttttag atatatgctc aggaacgagt gcagtagctt
    11161 ataatatgaa aagacacttt acggtttata caaatgatag tgaaaattat gcatatcaga
    11221 tatctaattc aattgttaag aataatgaaa gatataactt tagtcaatca gatgaaatga
    11281 tcataagaga aaaatataat attaatttac aaagattaaa taatattttt aacgatatgg
    11341 taaatcagga agatatgtta ttagaacaag aagatgcaga cggtttaata gagctatatt
    11401 caaattgccc atgtatatgg aaaaataatg ttgatattga aatagaaggc aatacaatac
    11461 agagtatata tgatttaaga aaatgtaaac gagacatacc gaatgtgtta ttcacattgt
    11521 attattcaag tagttacttc ggcataaagc agtctatgga gatagattct attagatatg
    11581 ctattgaatt tatagaaagt gaaataacaa agagcattct ttttacaagt ttatttttcg
    11641 caatgaaaga gtgtgttttt gctaaagatg gtcacatggc acagccatta aatatggaaa
    11701 caggcaagaa aagaatgtta agattaaaag caagaagtat atatgacata tttatagata
    11761 aattaaagga atttaatact gaggagtttg tattatcaca aaaggagaat aagtgcttca
    11821 ataaagactt tagaacatta ttagaaacag aagaaagtat aaaagatgaa gttggtttta
    11881 tatatataga ccctccgtat acagatatgc aatactcaag atattatcat attcttaata
    11941 cagttgtttt gtatgattac ccagaaatat cattgagtag aggaactata tcaaaaggga
    12001 tatatagaga aggtagatat caatcacctc ttagtcaaag aaaacatgca ttaaatgaga
    12061 ttgatttctt atttagtttc tgtaaggaaa ataaaataaa tgtagcatta agttatgcat
    12121 atccaataga tacagaaaaa caagcaacta atagatatac ggctagtatt gatgatttga
    12181 taaatgtagc caaaaaacat tttggagata atgttaaggt agtacatgaa gaatatagtc
    12241 atgcaaataa tagaaatagt gataaaaaga atgtacttga atatttaata atgggtatac
    12301 acaataagga gtaaatatgg ataaaattaa taaattaaaa gagaggatag ctaatataga
    12361 agcgacgaat aaaaatgata tatattcttc taacttatat tggtcacaaa aaccattcaa
    12421 tatatgtgat gaaattattg aacatttaag tgaagatgga gatataatat ttgatccgtt
    12481 tatgggatct ggggttacag ttatagaggc tttgaaaaaa gagtataaaa gaaatgctat
    12541 tgggtgtgat ataaatgatg caccaatatt tatagtaaaa acattattag aaaaatataa
    12601 tgtgcctaaa atagagaaag atattaaaga gctaaataat aaattaagta aattatcaac
    12661 ttactattca acaaaatgtc gtgaatgtgg agaagtagtt actatatcga aaactatatt
    12721 cgataaggag aataggaatg caaagtgtga gctaaagtct atacattata aatgtaagga
    12781 atgtaaaaat gactttaaag gtgaaccaaa tgaaactgaa atatctaaat tctttattga
    12841 tgaagaatta aagaatatag atgattttca attcatagcc aattcaaggc ttgcagttgg
    12901 cgaaggtgat acagtaagaa ctattttttc aaatagaaat ttaatagtat tagataaaat
    12961 attaggacat atagatgaat ataaaaatag tgaatcttat gagcctatga agtatatttt
    13021 attatctatt ttacatttaa gtaagattac agatacacat tctaattctc agtggccttt
    13081 atggattcct aaaaaagact gtgttgaaaa aaatgtagta gaattattca taaagaatag
    13141 taagaaaacc attaaggcat taaaactagc atctaaatat ttatgtgaaa atagagaaaa
    13201 tgtagaacta atcaaaaatc tagatagagg gaaatttaaa ttgataaaag atggagttca
    13261 aaatatagaa gatgatgtta tacctaatga atttgtggac ttgataataa cagatccacc
    13321 atatatggga caagttttat attcagagta tatgcagtta tataaacctt ttatggattt
    13381 ggaattcgaa ttagaaaatg aaatagttat atcaagtgcc ccagctagag ataaagggaa
    13441 agtattatat tttgatatga tgaattctgc atttgaaaat atatctagag tattaaaggt
    13501 aggtaaatat atgtgtatgt acttccatga tagtaatcta gatgtatggg ataatataat
    13561 agatataatg accaataata atttgaaata catgggacaa attcatgtaa gtaagaataa
    13621 aaacactctt aaaaacatac ttagtcctaa gaagtcgtta aatggagatt gtataatttt
    13681 ctttaagaaa gatttaaata taaactatga tataaatgaa gtagataata tagaagataa
    13741 gataaataat attgcacaag ctattataga tataaaagga tacgcatcaa ctccagagtt
    13801 atatgataat ggagcactag agtatataat atcaaacgga aaactaaaag agatttctaa
    13861 aaagtataag gatttaacaa gtatatttga gcaaagattt aattgggata aggaaagagg
    13921 ggtatggact gtataataaa taaaaagcat ctatagataa ttatagatgc tttttattta
    13981 tattttatag taaactaaat atttaataat ttctaatttt tatacaatat gttaaaggat
    14041 tggctatcca aagatattat ttaatataat tttttagcat taactctagt taaatcactt
    14101 ttagataatg aaacctgtaa tcttctgata ggagaaccct tttctaaaca tctattgatc
    14161 ttttcctgta acttcttacg aagaagagga actacatctg ctggttcagt tgagtagata
    14221 tgttcaactt ctatattttc tttatcaaca actgctaata ttatagcttt atcattataa
    14281 tatttattta gtacattatc agatatatct tgaaagttcc aagcatgatt tttagaaact
    14341 ttatattcat aaggattacc ttcactatca taggcatcat gagcaccttc tcgtccacct
    14401 tgctctgggt ttacttgatg gtttaattct aatgcaacta ttaattccca aaacttatta
    14461 cttgtaagta aatttttaac tcctgttaat tcttcaagtt cacttgaagc tttataaata
    14521 gcacttagat atttatcata aacaccattt tgagctgatg ggattatctt acttgcattt
    14581 aatctagact ctagttgatt agggctgaca tttatcattt ttctggaatt ttgtatactt
    14641 tgagtatact ttagtaaata ttcacacatt atatctccag gtatagagta ataccaatca
    14701 agctttccag ggtaaggaaa atcaacatca tttatatgag caaatataac tgccttagct
    14761 ttctttaatt ttgctatact tgtatccgaa aaatcattaa acgtccaaga atggttagag
    14821 ctacttttct tgaaatgttt atattcaaac tcatttccga attcatcttt tgcatctcta
    14881 gaaccactat gtccaggtat tagtatgtga ttaagtgaat tagcagtcat aacttcatat
    14941 attttgctat tagaaagtat atcatttact ttatattctt taaaaatacc atcttgtata
    15001 gcctttagtt catttatttt ctgtgttatt aaaacatttg ttatattatc tcttatcata
    15061 ttatcatagc tccttaataa attagtattt ttagataagt catttttaat tagatttatc
    15121 acataactat tgatgctatc atttttaaat gctactaaat ccaataaatc tttatgaaaa
    15181 tcatccttga gtcttatagt tagttttttc aagctatcac ctcggatatt aattagatgt
    15241 gccgtcgaga taagtataat taagcaggtt tataaagtca aggattaaaa aaattaaatt
    15301 atagatatat aaaagtatat atagcaatat agataaatag ctaaactatt aatcgtgggt
    15361 attattcttg cgattcgtct ccaccaattt tgaaaaaaga atgatgaaat acttggaaat
    15421 actagtcttt gttgactagt attttttatt ttggagaaga aatccaccaa ccgtacccca
    15481 accacagaaa atgaggtttt tttgggggta tattggggtg catttaggct gtataaaaag
    15541 tttttatttg tttattattt tatttgttta ttatgataat aggtaacata gaattttaat
    15601 atctacttta tgatataata tttttataaa tgtaagacag tttaagttgt taattagtaa
    15661 aaatatatat tatataagct tgataggagt tgaggtttgt aatggatgaa agaaaactag
    15721 aattttctat tttttgcatt gaagctttag cagaaaaatt gaatatagat tcagaaggag
    15781 tttataaact tcttaaacat gatactgata ttttagataa ctatataata ccttgctatg
    15841 aagcactaca ttctcaaagt aaaggttaca tagtggaaga tttaatgcaa gtacttaaag
    15901 aaaagggtgt gatataatga ttgtatatca tggttcatat tgtgtggtaa agacgcctag
    15961 tattaccttt tcaagagatt ctttagattt tggaaaagga ttctatgtaa ccagtataaa
    16021 gacacaagca attaactgga cagagcgttt taaaaaaaga ggtaaaaaag catatttaaa
    16081 tagttattct ttagatattg ataaattaaa agaaaactgc aaggtaaaaa tcttcgatag
    16141 ttataattta gaatggcttg attttataat agaatgtaga aatatgagtg atatatatat
    16201 gaaatatgac attataatag gtggtattgc tgatgataag atttacaata caatagaact
    16261 gtatcaagat aatcttattg aaaaagatga agctttaaaa agattaaaat actataaacc
    16321 aaatgaacaa atatgtataa ccaatcaaaa tgtaatagat aaatatttaa agtttaggta
    16381 tgaagaggag gtataatgat tatggaagca aataaaacat tattacaaat actatataaa
    16441 aaaatcatat tagaattttc caaacaaaca ggtaagagct tggaagaaag tatggattat
    16501 ttatatactt ctgaaacata taaacttata agtgagggtg tatcagatat gcactgtaag
    16561 ggtcatatat acttggctca agaattaatg ttagaaaatg gattaatgta ccataagggc
    16621 tatccaagat aaataattaa aactagatac gaaataagga tacaatataa tataaaatta
    16681 atttgtatcc ttattttgat tttgtaatta actatatgaa atcacaatat ttatatgtat
    16741 ttgattttta caatgaaaat attttatcca acttttctac agcttcaact ttcttatctt
    16801 ccataacttg agtatatata ttcaaagtta ttgatatatc ataatgaccc attaaaactt
    16861 gtacagtttt aggaggaaca tcattctcaa aaagtctagt agcataagtt ttcctaagac
    16921 catgaaactt tataggctct atatttaagc tatttaatat agactttaaa ttccttaaag
    16981 gtcttttatc atctataggg atgcctaatt cattgcaaaa tacataatta ttatttatat
    17041 ataattctcc agcctttaat atatctgtat tttgatttac tttatgctct ttcaatttat
    17101 ttaaaacatc ttttggaata ggtacagttc tgtaagaatt tgatgtcttt ggcccttgct
    17161 ctaatacttt aagctctcta tttccagttt tatctataaa atatgttctt tgaagagttc
    17221 ttttaactgt taaattagac tttttaaaat caatatcaaa ccatttaagt cctaataatt
    17281 ctcctaatct aagtccagta ccaagagcaa gtaaaaataa tacctctaat ttatgcccat
    17341 ctatagcttt tataaaactc ttttgttgtt caagagtcaa tatttcaagt ttgttttctt
    17401 ttttctcaac tggtatattt accatagtac aatagttacg ttgtataaga ccttgttttt
    17461 cagcttctga taagcaggtt ttcagtttag tgtttatttg ccttatagta ggtatacttt
    17521 tattggaatc aagcaatcta ttgtaatatt tttgtatgtg agatactctt aagtacttta
    17581 atttaacatt gcctaaatca gtatctttaa tataattacg atagatgctt tcatatcttt
    17641 caaatgactt gggttttaaa tcttttattc tatattcaaa taaccaattg taaaaccaat
    17701 gctctaaagt tattttatca tctgaaatat cagagcttaa ataatattgt tttttataag
    17761 catctaattt atctttaact tccttttgag tcttaccata aaattctttt ctcagtggtt
    17821 taccatcttc attatagcca atcattatac gggaacgcca accatcttta tattttccga
    17881 tagagccttc tccattagct cttttagttg ccatttttaa acagctcctt atatgattta
    17941 tagttatatc catcaaagaa tgtaatacat ttctctaata agaattttat aacttcatca
    18001 gcttctagtg tttcatagaa gtttagcatt aatgtgttgt actcttgttt atgtttagct
    18061 ggtattgata gaactcctat atcattattt ataagtatta ggtttgaggt aagtctagca
    18121 gttcttttgt tgccatcata gaagaattgg caataggaca accataaatt tattattata
    18181 gccttttcta atgggttagt tattttattt atgatattta tgtcatttac aaataaatca
    18241 tttaatagag tggcttctat gcattcatat tgagtagtac cagctatacc tatattacca
    18301 tttctgaaat ctccaacgat tagagcttca tcttttgcaa ctattttatg tatagagcaa
    18361 attgtatctt tatttaaaga taagttttct ttgttactta attttattac ataatcccat
    18421 gatgatttta tatttagtat ttgattttgg tcacttaatt tatgtccgcc tacggttata
    18481 ccatcaagta atgtttgtac ttctgggaat gtgaaggggt tattttctag gttacccata
    18541 tcatatacaa attcacttat cattcttcta atgtatgtta aagtatattt tttatctcca
    18601 tgaggtatat tagtagggaa tttatgtgtg tattttggta tatatgattt attcatgatt
    18661 ttagctcctt tcatatctaa tttttattta ttaactatga atgtataaaa gttaataggt
    18721 aaactcatat tttgaaaatg tgcaatacta atattcttca tcctcatctt caataagacc
    18781 attagagttt tcttgtataa aattttcaac aagaccattt atcacccaat ttagattttt
    18841 ggagaagtct ttgaagtcag agaagtttag gttaattggt tttattgtat ctgcattatc
    18901 tgtaatttca aacttttttt tatagtctgt ttttatatca taaccataat tttctaatat
    18961 gtgaattact aaatccaact ctttatcaaa tacttttaaa ttaatattat tatttgttaa
    19021 agtatctaaa tcattttctt caacaatgag tttatttata gcaattgata aagcagtagc
    19081 tatttttctt atcatatcta aactaggcat tctttgattt tgctcatatt tagctatagc
    19141 atgaactgaa caaccaatct tgttggctag ctcatccctt gacatattta tcgattttct
    19201 atattgttta atcttttctc caattgtatt attcatcata atttcacctt aaatatatca
    19261 tcaaacaatg attgtacttc taaacatgta aaggagttat tttctagatt atcccatatc
    19321 atatactaaa ttactcataa ctctcttagt gtatgttaga gtatattttt tatctccatg
    19381 aaataaatta gtaggggatt tatatgtgta ttttagcata tatgatttat tcataattgt
    19441 aactccattc atagtttttt atcattgctt tagatagtat tgatattatt ttatatttta
    19501 gttagtagtt tttttatttc tttaagtgta ttttcaagct tttcattttt ttcctttagt
    19561 tcgttatttt cttgtaataa tttaaagtaa
//
LOCUS       DACULQ010000062        19347 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.062, whole genome shotgun sequence.
ACCESSION   DACULQ010000062 DACULQ010000000
VERSION     DACULQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.062"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            168..689
                     /locus_tag="JA154_08890"
     CDS             168..689
                     /locus_tag="JA154_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7995980.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            723..1499
                     /locus_tag="JA154_08895"
     CDS             723..1499
                     /locus_tag="JA154_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7995981.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1841..2794
                     /locus_tag="JA154_08900"
     CDS             1841..2794
                     /locus_tag="JA154_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HAT7995982.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2812..3831
                     /locus_tag="JA154_08905"
     CDS             2812..3831
                     /locus_tag="JA154_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HAT7995983.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3862..4113
                     /locus_tag="JA154_08910"
     CDS             3862..4113
                     /locus_tag="JA154_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995984.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4395..6200
                     /locus_tag="JA154_08915"
     CDS             4395..6200
                     /locus_tag="JA154_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088981.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAT7995985.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6340..7686
                     /locus_tag="JA154_08920"
     CDS             6340..7686
                     /locus_tag="JA154_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HAT7995986.1"
                     /translation="MIFMINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPN
                     DWDITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDS
                     IEFTNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDA
                     LRMLRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLL
                     KSTGLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGK
                     KVTKTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRK
                     IIKKLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECF
                     NLKDLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7919..9232
                     /locus_tag="JA154_08925"
     CDS             7919..9232
                     /locus_tag="JA154_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT7995987.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9404..10579
                     /locus_tag="JA154_08930"
     CDS             9404..10579
                     /locus_tag="JA154_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HAT7995988.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10682..11701
                     /locus_tag="JA154_08935"
     CDS             10682..11701
                     /locus_tag="JA154_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HAT7995989.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11731..12351
                     /locus_tag="JA154_08940"
     CDS             11731..12351
                     /locus_tag="JA154_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAT7995990.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12443..14290
                     /gene="dnaK"
                     /locus_tag="JA154_08945"
     CDS             12443..14290
                     /gene="dnaK"
                     /locus_tag="JA154_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088975.1"
                     /note="heat shock protein 70; assists in folding of
                     nascent polypeptide chains; refolding of misfolded
                     proteins; utilizes ATPase activity to help fold;
                     co-chaperones are DnaJ and GrpE; multiple copies in some
                     bacteria; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAT7995991.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSESEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14501..15655
                     /locus_tag="JA154_08950"
     CDS             14501..15655
                     /locus_tag="JA154_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088974.1"
                     /note="chaperone Hsp40; co-chaperone with DnaK;
                     Participates actively in the response to hyperosmotic and
                     heat shock by preventing the aggregation of
                     stress-denatured proteins and by disaggregating proteins,
                     also in an autonomous, dnaK-independent fashion; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAT7995992.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            15867..15983
                     /locus_tag="JA154_08955"
     CDS             15867..15983
                     /locus_tag="JA154_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7995993.1"
                     /translation="MGTDVALPNDEENFIVIYKIKLDTFSARLSTGKTHELG"
     gene            16621..17556
                     /locus_tag="JA154_08960"
     CDS             16621..17556
                     /locus_tag="JA154_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAT7995994.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKKRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EGLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17592..18965
                     /locus_tag="JA154_08965"
     CDS             17592..18965
                     /locus_tag="JA154_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7995995.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19153..>19347)
                     /locus_tag="JA154_08970"
     CDS             complement(19153..>19347)
                     /locus_tag="JA154_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HAT7995996.1"
                     /translation="YTGIKFINNDLNISKTSTSMFLHRNTLVYRLEKINEILGFDLKN
                     FENAMIFYLSVKSYFLYKKI"
BASE COUNT         7668 a         2130 c         3522 g         6027 t
ORIGIN      
        1 caatataact aatatgttag aaggttatat gtaattaatg tcagtgttta tatagaatag
       61 catttgtagc atttattata gttgtaatta ctatttatat catagacttg aatagtctaa
      121 aaaaatagac tataatattt ttatagacta aagaaagggc tgataagatg gacatagcaa
      181 aactaatttt aaatcctgca aggcttcgta ttttacaata tattcgactt tatggcagtg
      241 ttcgtacatc tgatattgta aagtatttaa atgatattcc tcgtgcaaca gtttatcacc
      301 atgtaaaaat tttagaagag aacaatatga ttgaagtaat taaagaaaat cgtgtgcgtg
      361 gtactattga aaaggtttat gcactaaagg aatatacaac atctatggaa ggagagacct
      421 tcgttgcact ttctacagca ttttatgtag gtctgatgca ggaaatgaat gaatacttta
      481 gcagaaaaga gcaagaccat aaaaaggata atgtattttt ctcttcagca ttactatata
      541 tgagtgataa tgaatatgaa aatcttctta aaagtatagc tgatttattg aaaccatata
      601 tagaacaaaa acctaaatca aatctaaaac ttagaaaact ttcaattata tcttctccac
      661 ctgtagctga taatgataaa acaaaataac atgcaaccca tttttataaa aggaggttat
      721 atatgaaatt tcatgagttt ggaaataaaa acagaccaca tattatgttg attcatgggg
      781 gagggaatgc atggtggaac tatttgcgac aagcagaagt tttgtctaac aaatatcatg
      841 tgatacttcc tacattggat ggtcatggag aagaatatga aacaacatac atttctacag
      901 aggatacagc agataagttg atatcatata tagatgaaaa ttgcagtgga catcttttcg
      961 ctttgtgtgg ggtatcactt ggtggtcaaa ttgtcatgga acttttatct cgaaaaccta
     1021 atattactaa aaaagcaatt attgatggta gtatttgcta cccaagacct cttatggcaa
     1081 ggttttgtat tgctggtatt cgattttttg gaggactttt gtttagtgag aaggcttgtc
     1141 gctttcaaat tgctatgatg ccaaaattgt tacctgaaaa tatgcaatat ccagatgaaa
     1201 taaaagcgta ttatatgaca gatatgccac atgtaagaaa agaaacattc tataatatgt
     1261 atcgtactta catgatgaac tatacactta aagagaatgt taaaaaaacc acagcacaag
     1321 taatgtactg gtatggagaa aaagaaatga agtgtgtcaa aaaatctgca aaactgtttc
     1381 agtcttatgt gccctcttgt gagatttatg aagctaagag ctataatcat gggtatttat
     1441 cactttatct tccaaatgaa tggttggaaa ttgcagaacc atttttccaa aaggaataaa
     1501 ggtgttaggt gattattgta taattattgc tgttttttta tatagtaaat aaattaggta
     1561 taaatataaa aaattatttt agttaatata ttagtgaaag cttaatagga aaattataaa
     1621 tagattaaag gtagcaccta tataaatagt tgctaccttt ttctgtttgt aaataaatga
     1681 tagataagaa ggtttactga caaatcactg actaaaccca attttcttac attgaaagcc
     1741 ttaattttac ttgttttaaa taaatttaca atttcttgtt ataaaatact aaattttgtt
     1801 aacaattata attagcattt aatttatact ggaggtttat atgattagtg taagaacaga
     1861 tttagcttta gaagctagtg aaatgtgtga aaagtctcaa gaaggaagta gtattcctgg
     1921 tgtaaaaata gaaactaaag aattagaaaa ttgcattgta actaaagttg aagtaataga
     1981 caggcaaggc gcagagataa tgaataaaga tataggaaag tatataactc tggaaagtaa
     2041 tcttatgaag tttgatgatg atgagtcaag agaagaaatg ataagttatt taaaagagga
     2101 gttagtagat atatttggcc aagacaaaaa taaaaaaacc ctggtaattg gtcttggaaa
     2161 tagaaatatt acttctgatg cgttaggacc aaagagtgta tcaaaaacac ttgtaactag
     2221 acatcttttt aaaaattata ataaagacta tgatgatgat ttcactgagg tatcagcttt
     2281 aagcccagga gttatgggtg ttactggaat agaaactagt gaaatagtaa aatcattggt
     2341 tgagaaggtt aagccagata gagtagtagc aatagatgca ttggcttcaa gaaaaatgga
     2401 gagagtaaac tctactatac agatatcaac agcaggaata tcacctggtg gaggtgttgg
     2461 caatactaga aaatctctaa ctaaagaaac tttaggcgtg gatgttattg caataggcgt
     2521 tccaacggtg gttgatgcag caactcttac aatagatgtc ttagatatgg caatagacaa
     2581 tttaatagct caatcagaag aaacagaaag tttttatgaa atgttgaaaa aacttaaaga
     2641 agaagaaaaa tatcatctaa taaaagattc gttagaccca tatgacaaga acttaatagt
     2701 aacaccaaag gatatagatg atacaatcga aaatctatct ataataataa gtgaaggctt
     2761 aaataggtct ttacatccag gtagattggt ataaaataag gaggagcaat tatgtttaaa
     2821 aaatgcatta aagtagtaac cttgactttt atattagtat gcattttacc aggaaaatca
     2881 ttagctttga atcaagatga ttttttaaag tttttagtaa attcatctta tccagaagct
     2941 aaagtcgaag gaaatgatac agaaaataaa aagaataata aaaataaaga aacaagtaag
     3001 gaaaataagg aagaaagtaa agaaggaaac actaaatcta aagatgcaag taaagttgat
     3061 aataaaaaag agagtgaaaa agaatatata aaactttatg ttggtaaaga aaatgttcca
     3121 gatattgagt caaaaaattc agacacaact gaaactaaca caacatctag ttcagattat
     3181 aaggatgatt taagagtaac caaagaaaat ccaagaattt tgatatatca tactcatgga
     3241 tgtgagactt attcaaattc acctgatgga aattatcatt caagagataa gaaaaattca
     3301 gtaatggaag taggaagtgc attaactagt gctttagata gcaaaggatg gggtgtagtc
     3361 catactacta aataccatga ctatccatct tataataact cttatgcaag tagcttaaaa
     3421 acaatacaaa gtatattacc taaatataac tcagtggata tagcgataga cttacataga
     3481 gatgctaggg atttaactaa tccagccact aaggaaaaag accatttaaa atatacaact
     3541 atgataaatg gagagagagt atcaaagttc ttctttgttg taggaggtaa aaatacaaat
     3601 agaaaacaac ttagagcttt agcagaagat ataactgctt ttgctgagaa aaagtatcct
     3661 ggtctagtat ctccaatagt agaaaaagac tatgctagat tcaatcagtt tgcagttaag
     3721 aatcatatgt tagtagaaat agggaataac gcaacaagtg tagaagaatc taaggctact
     3781 acaaagtatt tagccgagat tttggatgaa tattttaaac aaaaaaatta gagctttaag
     3841 ttataattag gagcatgcta aatgagccga ttagaaaaaa atatagaaag taaaagtaaa
     3901 ctaagaaaat acaagtatat tatgagaatc ttatttttga ttactatgat tatatgtaca
     3961 tcagtttgtg tatttatagt ggacagtaat gcaaaaagta tgctaggaat gaaacaatat
     4021 gattttccaa gtattaaatt tgatataaca aaatttgatt ttgacatacc aaattttgat
     4081 atacatgaaa tcataaaaaa atttaataag tagtaataag tgaataaggt ttattgatta
     4141 aaataagcct tattttagtt atatattttt aatgtataaa ataaaaattc attaactgta
     4201 ttttatagga tttactttaa gtttatgata ttatataata tttagtgtta gacttgataa
     4261 tccacatata atgtggataa ttttgtttag tgccaattag tttatattca aaaattttaa
     4321 atttggtata taaagagttt ttagataatg taaaataata actataagaa taatcaagag
     4381 gagggaattt taaggtggac aataaacaaa gtagaactag aaattttagt ataattgcac
     4441 atatagacca tggaaagtct accttagctg atagattaat acaacaaact gggcttgtta
     4501 gtgaaagaga catgaaaagt caattacttg acaatatgga ccttgaaaga gaaagaggaa
     4561 tcactataaa gcttcaaaac ataagattaa tgtataaagc gaaggatgga aatgaatatt
     4621 atttaaatct tatagataca cctggacatg tagactttaa ctatgaggta tcaagaagtt
     4681 tagctgcatg tgaaggagca ctattagtag ttgatgcagc tcaaggtgtt gaggcacaaa
     4741 ctttagccaa tgtttatctt gctatagacc aagatttaga aatattacca ataataaata
     4801 aaatagattt accaagtgca agaccagagg aagtaaaaaa tgaaatagaa gatttaatag
     4861 ggcttgattc aagtgaagct cctcttattt cagctaaaac tggtttaaac atagaagatg
     4921 ttttagaaga tattgtaaaa aatgttcctc cacctaaagg agataatgaa gctccattaa
     4981 aggcattaat attcgattct tactatgatg cttacaaagg tgttgtagct tatgttagag
     5041 tatttgaagg aactgttaaa aagggtatga caataaaaat gatgaatacc aataaaaagt
     5101 ttgaggtaac tgaagttggt gttatggctc caggtcaaac tgaacttagt gaattatcag
     5161 caggtgatgt tggttatatt gctgctagta taaaagatat aagaagttgt cgtgttggtg
     5221 atacaataac agattcaaat aatcctacag aagaaccttt accaggatac aaaaaagcta
     5281 ctccaatggt atattgtggt atatatccag gagaaggaga aaagtatgag aatgttagag
     5341 atgctcttga aaagcttcaa gtaaatgatg ctgcacttga atatgaagct gagacttcag
     5401 cagcactagg atttggtttt agatgtggtt tcttaggtct tttacatatg gagataatgc
     5461 aagaaagact tgagagagaa tttaaccttg atataattac tacagctcca tctgttatat
     5521 atagagttac aaaaatggat ggagaggttg taatgataca aaatcctgca aacttaccag
     5581 aaccatctga gataaagatg atagaagagc ctatagtaaa aggagatata atagttccta
     5641 aagactatgt gggtgttgtt atggagcttt gccaggaaag acgtggaaat atgttaaata
     5701 tggaatatat agatgataga agagtaatgc ttcactatga ccttcctctc aatgaagttg
     5761 tttacgattt ctttgatgct ttaaaatcaa gaactagagg ttatggttct cttgattatg
     5821 aggttaaagg ttatgtagca tctacacttg taaaacttga tatattaatc aataaagagc
     5881 aagtggatgc acttagtttt attgtccatg agacacgtgc tttccctaga ggaaaggcaa
     5941 tgtgtgagaa attaaaagga gaaattccaa gacatcagtt tgctatacca atacaagctg
     6001 ctgttggtaa taaggttatt gcaagagaaa caataagcgc tctaagaaaa gatgttcttg
     6061 ctaaatgtta tggtggagat atatctcgta agaagaaact tcttgaaaaa caaaaagaag
     6121 gtaagaagcg tatgagacaa attggttctg ttgaagttcc tcaaaaagca tttatgtctg
     6181 tgcttaaatt agatgagtaa atccttataa atttagataa agatatttaa aagctagagt
     6241 gattttagaa ttatttctaa attagtctag cttttttatg gtatatttta agtaaactca
     6301 tagagaatat aagtaagaat attaaagaag aataaaaagg tgatatttat gattaatata
     6361 gaaattccta aaaaagtaga ctatataatt aaagaattag aaaagaatgg ttatgaagct
     6421 tatatagttg gaggatgtgt tagagattgt ttattagaaa gagttccaaa cgattgggat
     6481 ataacaacta gtgctagacc agaagtggta gtagaattat ttgaaaaaac aattccaact
     6541 ggtatacaac atgggactgt aacagttatg attgaacatg aaccatttga agtgacgact
     6601 tatagaattg atggaaatta tagtgatggt agacatccag attcaataga atttacaaat
     6661 aacatagtta aggacttgtc aagaagagat tttactatca attcaattgc atacaattca
     6721 aagacaggat tagtcgaccc atttaatggc tatgaagata tacaaaataa gtatatacgt
     6781 tgtgttggaa atccagtaga tagatttgaa gaagatgcac ttagaatgct tagagcagtt
     6841 agattttcag cacaactcaa ttttaaaata gctgaaggta ctaagcaaag tattcataaa
     6901 aaagcagatt tgataaaaaa tgtttctatt gaaagaatcc agacagagtt taataaaata
     6961 ttagtatctg attcatctaa attaaactta ttaaaatcaa caggtttgtt gaaatttata
     7021 attcctgaaa tatgtgaatt agaagatgtt actcaacaca atccatatca catctatgat
     7081 gtacaaaaac atactttaat agctacagaa gtaattgaag atgaactgta tttaaaactt
     7141 acaatgttgt ttcatgattt aggtaaaaaa gtaactaaaa caactgataa aaatggtgtt
     7201 gaccattttt atactcatag tagagaatct gtaaagattg caaagaagat tttgaaaaga
     7261 cttaagtatg ataattatac tattaataaa gttttaatat taatacaata tcatgattat
     7321 agaatagaac ctaaaaggaa gataattaaa aagttattaa ataaacttga agatgttgaa
     7381 ttatttgaag atttaataaa agttaattgg gctgatacac ttgcaaaaaa tccaaagtat
     7441 gcaaaacaaa agatattaaa tcttattgag tgtgaaaaag aatttaaaca tataataaat
     7501 caaaaggagt gctttaatct taaagattta gctattaatg gaaaagattt gatatctata
     7561 ggtataaaac caggtaaaga tataggatat attttaaata agatgcttga aatagtcata
     7621 aataatccag agttaaatga aaaagaaata ctaaaagaaa aagcattaaa tatatataca
     7681 ttttagattc aaatataaaa aatttaatta ctttaaaaaa catagaataa aatacttatt
     7741 tagataaaat aaataatttt tagagaatat tttagaagtt acgattgtat gatttttaaa
     7801 tgcataggca tttactctag gactatagac taatataaaa aaaaactttg ggaatactaa
     7861 aaccataaat ttataaaatt aatattttat atactgtaaa acagtgagga gggacagtat
     7921 gcatactcaa ctacaaaaga ctattggtct ttcagcagct ctttctactg ttgtaggtat
     7981 ggttataggt tcgggagttt tttttaagcc acaagcaata tacactacaa ctaatggagc
     8041 tccaggtcta ggtattatag cgtggctttt aggtggtttt ataacaataa ctgctgggct
     8101 tacagctaca gaaatttcag ctgctatacc aaaaactggt ggtatgatga tttatatcga
     8161 agaaatatat ggagaaaagt taggtttttt aactggatgg atgcaaactg tattattttt
     8221 cccaggtaca tcagcggcat taggagtaat atttgctcaa caagcatctg aacttttagg
     8281 aatgagccca aataatatgg caaatgtact tcctatagca attggagtta tactattttt
     8341 agcgttatta aatataatag gttcatcctt aggaggaaaa gttcagacag tagccactat
     8401 tggaaagatg atacctttaa ttttaataat agtctttgga tttattaaag gtcaaagtag
     8461 tgaagtatta aatccatttg taggagatgg tgtaaatgca tcaaatgcac ttggacaagt
     8521 attaatagct actttgtttg catatgatgg ttggataaat gttggagcta tatcaggaga
     8581 aatgaagtct ccagagaaag atttaccaag agcaatagtt ggtggtttat cattagttat
     8641 ggcagtttat attataataa atatagctta tctttgggta gtaccagcaa gtgaattagc
     8701 aacagttact tccccagcta cattagtagc aacaagattg tttggaaata taggtggaaa
     8761 agtaataact gtgggtatat taatatcagt atttgggaca ttgaatggat atttattaac
     8821 aggttcaagg ataccttata cattagctga aatgggaact ttaccagcat ctaaaactct
     8881 tttaaaagta aattctggag gttctcctgt aaattcaata ctacttataa ctgtgttagc
     8941 ctgtgtatat gcattatctg gtcaatttaa tcttttgaca gacttaacaa tattttcaat
     9001 atgggtattt tatgtattga cttttatagg tgttatgagg cttagaagac aaaagccaga
     9061 tttacacaga ccatataaag tccctttata tccaataata ccaattatag ctatattagg
     9121 tggactgttt gtaataataa atcaaatatt gacttcaaca gtcatatctt taggcggaat
     9181 atttataaca ctattaggtt taccagttta ttattatatg aaaaaaagat aactacttaa
     9241 tttaagttag ataaatttgg gtaaaggtga aaattaactt ttatccaaat tttttatact
     9301 gttatacaat ataagtatta tatattgctt gatgtgctat tttgtttgat ttataattta
     9361 gtaattgatg ttttaagata atttaagaaa tgaggagtga aagatgctag gactttatgt
     9421 acatattcca ttttgtgtga aaaaatgtaa gtattgtgat tttaattctt ataaaatgga
     9481 catagattca aaaaaaagat atatagaaga cttaaaaata gaaatggaat tatatagtaa
     9541 taaattatat aaagataata aatataaaaa taaagaatgc tgtagtctaa ataaaaatga
     9601 caaaataacg agtatatttg tagggggagg tacacctagt atactaacct ctgatgaaat
     9661 aagagaagta tttataagta taaaagaaat gtttgatata gatgagaatg cagaaataac
     9721 tatagagtgt aatcctggga cattaacatt agaaaaatta aaaactatga aagagattgg
     9781 cataaataga cttagtatag ggctacaagc tattcaagaa aagcatttga actttatagg
     9841 aagaatacat acttatgaag aatttgagaa aaactataaa gatgcattaa gtgtaggttt
     9901 taaaaatatt aatatagatt taatgtacag tcttccaaat caaactctat gtgattggaa
     9961 ggaaactctt gaaaaagttg tacacttaaa tccaacacat atatcagctt attcccttat
    10021 actagaagaa ggaactgaac tatacaatat gtatgaaagt aataaatttg aacttataga
    10081 tgaaaatgta gatatagaaa tgtatgagta tacaataaac tacttaaagt caaaaggata
    10141 taatcaatat gaaatatcca actactcaaa agaaggctat aattgtgagc ataatatttt
    10201 atattgggaa tgtgaacatt atatagggat tggagcagga gcttctggct atattaatga
    10261 gaacagatac aataatgtag aatctttaga agattatcat ttaagtttgg taaaaagaga
    10321 aaaaccaata caagaaaatg aaattctttc tgagaaagat atgatagaag aaaagatatt
    10381 tatggggctt agaatgaata aaggcataaa atttgaagat tttaaaaaaa aatttggaat
    10441 agattttaga gagaagtata ataagcaaat agaaatgcta ttagctagaa agcttataaa
    10501 tcagagcttt gaaggtatac aattaactca aaaaggaaga gaaatatcta atagtgtatt
    10561 tatagaattt atggaataat ttttaaggtg ttgacaaaat aaatttataa tggtatataa
    10621 tatatataat ctttttagca ctcgaactta gtgagtgcta acagctagga ggtataataa
    10681 tatggaatta aatgaaagaa aattaaatat cctcaaagct atagttaaag attatataga
    10741 aacagctgaa gctataggtt ctagaacaat atctaaaaga catgatttag gtgttagtgc
    10801 tgctaccata agaaatgaaa tggctgacct tgaagaatta gggtatttaa ttcagcctca
    10861 tacttctgcg ggtagagttc catctgaaaa aggctataag ttatatgtaa actctcttat
    10921 gagtaaaagc gaattggatg ataatgataa gattcttatt gaacaatgta tgaatcacaa
    10981 tataaatcat ataaaagaat tgattcatga aacttctaag ctattatcgc aactaacaaa
    11041 ctatacaact gtagcagtta ctaagagttt aataaatcaa agtgttataa agcacataca
    11101 gttggttgct atgaatgaca ataatatttt acttatagtt gtaacagata aaggggactt
    11161 aaaaaaagct aaccttacaa ctaatgtata tttagatcaa tctaagttaa atttgatttc
    11221 tgataatctt acaaggaagc tcttaggtaa aagtataaca gatttggatg ataatctaat
    11281 tgcttttatt aaatacgaaa taagtgagta ttctgggctt atagatgagc ttttaaatgc
    11341 tttgaactca aatatgaaag aagaagattt ttctctatca ttaaatggag ctactaacat
    11401 atttagttat ccagaattta atgatgtatt aaaagcaaag tcatttttaa atatgttgga
    11461 aaaaaaggaa acaatagctg acataattaa atcaaaggga atacaaaaag acaatctaaa
    11521 tataataatt ggtagtgata atgattgtga actggcacag gattgtagta tagttacagc
    11581 aacttataat gtagatagag acttggttgg gcgaataagc tttataggcc ctaccagaat
    11641 ggattatgct agaatttact ctataataaa ttatatgagt ttattaatta atagaaagta
    11701 aagaaatttt aaattgcgag gtgtaaagtc ttggataaaa aaaatgagca acaggaagta
    11761 agagaagaaa atgatacttc tattaaccaa gaatcggaaa ctcaagtaga gttagaagag
    11821 gaagttgtta atgaagaatg tgaaacatca agtgaaaaaa ctgatgaaaa agaagttgat
    11881 gatgaaaatg taacagatat aaattctaag ttagcagaaa aaaaattgca ggatgagttg
    11941 gatgaattaa atgataaata tcaaagactt caagccgaat atgcaaacta cagaagaaga
    12001 actcaacaag agaaagagac aataggcgta tttgcaaatg aaaaaataat aactgaattg
    12061 ataccagtta tagatagtat ggaaagagcc ttagatgcat gtgaagataa ggaagatact
    12121 atgtataagg gtataagcct tgtacataaa caattaatag acactttagt aaaatttgga
    12181 gtagaagaaa tagaagcaga atctaaggaa tttgacccta atctacatct agcagttatg
    12241 caagaaagtg tagatggagt tgaagcaaat caaatagtaa tggtacttca aaaaggatat
    12301 aagctaggaa ctaaagttgt aagaccttca atggttaaag tttcttgcta agtataaatt
    12361 aaaggttaaa ttatttaacc taatataaaa tattaaaata agtaaaactt aattatataa
    12421 ttactcgagg aggattttag tcatgggaaa aataatagga atagatttag gaacaacaaa
    12481 ttcatgtgtt gcagtgttag aaggtggaga agcacaaata atagcaaata gtgaaggtat
    12541 gagaactact ccatcagtag tagcatttac aaaagatggt gaaagaatag ttggagaacc
    12601 tgcaaaaaga caagcagtta caaatgcaga taaaacaata acttctataa aaactcatat
    12661 gggaactgat tataaagtaa atatagatgg aaaatcatat actccacaag aaatatcagc
    12721 aataatatta caaaaattaa aatcagatgc agaaagttac ttaggacaaa cagtaacaga
    12781 agcagttata acagtaccag cttactttac agatgctcaa agacaagcaa ctaaagatgc
    12841 tggtagaata gcagggcttg atgttaagag aataataaat gagccaacag cagcagctct
    12901 tgcttatggt atggataaat tagaccaaga aaagaaaata ttagtatttg acttaggtgg
    12961 aggaactttt gacgtatcta tacttgaaat aggagatgga acttttgaag ttttagctac
    13021 agctggtaac aatagattag gtggagatga ctttgaccaa atagtaatag attatttagc
    13081 agaggaattt aaaaaagctg aaggtgtaga tttaagaaat gataaaatgg ctcttcaaag
    13141 attaaaagaa gcagcagaaa aggctaagaa agaattatca tcaacaatga gttcaaacat
    13201 aaacttacct ttcataactg ctacagcaga aggaccaaaa cacttaaata tagatttatc
    13261 aagagctaaa tttgaagaat taactagagg tttagttgaa aaaactatgg aaccaactaa
    13321 gacagcttta caagatgcag gactttctac aggtgatata gatgatgtat tattagtagg
    13381 tggttctaca agaataccag cagtacaaga agctgttaag aagtttatag gaaaagaacc
    13441 tcataaaggt ataaacccag atgagtgtgt tgcagcaggt gcttctatac aagctggagt
    13501 tttagcaggg gatgttaaag acttattatt actagatgtt actccattat ctcttggtat
    13561 agaaactatg ggtaatgtaa tgactaagat aatagaaaga aatactacaa taccaactaa
    13621 gaagtctcaa atattctcaa ctgctgctga taaccaaaca gctgttgata tacatgttct
    13681 tcaaggtgaa agaagtatgg cttatgacaa tacaacttta ggaagattcc aattaacaga
    13741 tataccacca gcacaaagag gaatacctca aatagaagtt acttttgata tagatgcaaa
    13801 tggtatagtt aatgtttcag ctaaagattt aggtacagga aaagagcaaa aaataactat
    13861 aacatcaaac actaatttat cagaatcaga aatagagcaa aagataaaag aagcagaaat
    13921 gaatgcagaa gctgacaaac aaaagaaaga aaaaatagaa gcattcaatc aagctgaatc
    13981 tactatatat caaactgaaa aaactttaaa tgaacttgga gataaaataa gttcaggtga
    14041 aaaagaggat atagaaaaag ctatagcaga cttaaaagct gtaaaagata atcaagatgc
    14101 tacagcagaa gaacttaaga aagctacaga tgaagtaatg actaagttcc aaaaagtatc
    14161 tcaagaaatg taccaaaaag cagctcaaga acaacaagcg gcacaaggtg cagaacaagc
    14221 acaagacaat ggacctaaag atgacaatgt agtagatgct gactttaaag aagtagatga
    14281 agataaataa gctatataat atgtaacact aaatgataat tggaacatat atgatttgtg
    14341 aaaatcatat agatgttaaa gatatatgga caatatacat cataaagaat ataatatatc
    14401 atagattata taacttaaag tagaatataa gaattaatga tagcttgtag tttattattc
    14461 tacaagctat cgttttgtaa aatgaaggtg gtgtcaattt ttgagtacaa aaagggatta
    14521 ttatgaagtc ttaggtatca gtaaaggtgc agaagctcaa gagataaaaa aagcttatag
    14581 aaaattagca atgaagtacc atccagatag aaatcctgga gataaagagg cggaagaaaa
    14641 atttaaagag ataaatgaag cttatgaagt tctatctgat gatacaaaga gaaaaactta
    14701 tgaccagttt ggacatgatg ggctaaatgg tcaaggcggt tttggtggtc aaggaggatt
    14761 tggcggtcaa ggctttggtg gttttgaaga tatgttcgga gatatatttg gtgacatgtt
    14821 tggaggaagc ttcggaggtg gaagagcaag aagaagaggg cctcaacgtg gtgcagatat
    14881 aagacagagt gttactatat cttttgaaga agctgcattt ggtaaaaaga tgtctataaa
    14941 agtaaataga agtgaagaat gtgaagaatg taatggaact ggagcaaaac caggaacatc
    15001 aaagaaaact tgttcaactt gtaatggaac tggtcaagtt agaactgttc aaagaactcc
    15061 atttggtaat atagcaagtt caagaccatg tagtgcttgt aatggaactg gagaagtgat
    15121 tgaatctcca tgtagtaaat gtcatggaac tggaaataca agaaaagtta agactataga
    15181 agtagatata cctgctggta tagatgatgg tcaaatgata aaactttctg gtcaaggtga
    15241 agtaggagaa aaaggtgctc caagaggaga cttatatatt gtagttaatg ttaagtcaca
    15301 tccactattt actagagatg gaaatgatat ttattttgaa atgccaatta catttgttca
    15361 agcaacttta ggtgatgaga tagaagtacc tactttggat ggaaaagtta aatatagtgt
    15421 gccagagggg acacaaactg gaactgtatt tagacttaaa gagaaaggaa tccctagaat
    15481 tagaggtaat tcaagaggag accaatatgt taaggttgta gttgaaattc ctaaaaaatt
    15541 aaacgataag caaaaagaac tattgagaga atttgcgaag gagtgtgggt cgaacgtcca
    15601 cgaaaagaag aaaaccttcg gacaaaagat agaagatatg tttaagaaaa agtagttaaa
    15661 gtagccaata tataatcata tttttaaaat cttagttatt tataagattg tcaaaggaga
    15721 tattatgatt tttatattgg ctatttttgt ataaaaaata aatgtgcata aaataaaact
    15781 gaatataaat aattttctga aaaatacttg tcctaaaata tataatatgc tataatataa
    15841 tcaaataaaa gaaaggggtg attccagtgg gaacggatgt ggcactgcca aatgatgaag
    15901 aaaactttat tgtaatatac aaaataaaat tagatacttt tagtgctaga ttaagtactg
    15961 gaaaaactca tgagctggga taattattgt aattacctag actcaaactt ttagtagtaa
    16021 aatagtaata ttatatagac aaggtatcta atttttaaat cttttgtatg aattaaaatt
    16081 tttataaagg gtatatctga ttagaatgta attagggaaa cctagttgtg aattactttt
    16141 agtatataca tgtgtaaaga aatatgtata tggtattggg tattgtgcta gatgtgtatg
    16201 taaaggaata tgtgtctagt tgggtatatg taaagaaata tacttataat ctatgaaaaa
    16261 tttttagtat aaattgctaa aacattgaga ttgtttttta tagaaagtta gtttatacca
    16321 actaaaaatg tgcttaagat ttgtttgatg tttaattata aataaggttg tagataaaaa
    16381 aataagtaaa gacaaaggtt taaaatactt gctttaaata tagtttgttt tgaacacttg
    16441 ccaaattaaa ataattaata aaatagacat cttctcatag taaaatatac tgtgagagga
    16501 tgtttttgct ttgttcataa acttttatga aaaatttgca tagtgtcaca ttttaatata
    16561 ctatatatat aataaaatat agataaaatt aaatgattca aaagagaaag gtgaaaagag
    16621 atgtattata taggtgtaga cataggagga acaggtatac aagcaggagt agttgataac
    16681 tatgggaaga taatttttag gtcagagtgt aaaacagtaa ttgaaaaagg atttgaagga
    16741 atacttaatg acataaaaat aatgatttat aagttgttgg aagataataa acttactatg
    16801 agtgacataa aatctattgg atttggtgtt ccaggtttta taaataaaga aggattagta
    16861 acttgtgtaa atttaaaatg gaataaaaaa gcttttaata aagagttaaa aaaaagattt
    16921 ccagatgttg aaattcatgg agaaaatgat gcaacagtag cagcacttgg agaagcaaag
    16981 tttggaagta tgaaaggtgc taatgttgga gtattatata ctttaggaac tggtgtaggt
    17041 ggaggaatag taattaatca aaaagtcttt tctggagcac atggattggg ttcagaaata
    17101 ggtcatcaaa ttataggaga aaactatttt aattgtaatt gtggaaataa tggttgtgta
    17161 gaaacttttt gttcagcaac tgcaattata aagtattctc aaaaactgat agaagaagga
    17221 gaaaagagta ggattttaga tttggcagag ggaaatttag aaaatgtaaa tgcaaagatg
    17281 gtgtttgatg catatagaga aaatgattta gtggcaatta aagtaataaa tagatttaaa
    17341 gagtatttag caaaaacttt tgcaaacaca ataaattctt tagacccaga aattatatct
    17401 ataggaggag gcatttctaa atctagcgat ataatacttg atggaataga aggtttggta
    17461 agaaaatttg tattatacaa aacagaagat atagctacaa ttacatgtgc aactttaggt
    17521 tcagacgcag gaattatagg agcagctttt ttataatgta aatgaactgc ttattggagg
    17581 cgtgatgata tatgggtaaa acaaatgcta gaagaacgct ttttttgata gcaataggtt
    17641 caggaactat gctgaatcct cttaattctt ctatgatttc acttgcttta catagtatac
    17701 aaaatgattt tcatattagt tttacaactg tatcttggat tgtttcagca ttttacctag
    17761 ctagtgctat tgcacaacca gtttcaggta agataggaga tttaataggt agaaaggtct
    17821 tatttttgag tggtttgatg cttgtaattt tatcagcatc catagtacca cttgttcaat
    17881 cattctttat attgattttt ataagagtaa ttcaagcaat tggaacaagt accctatatc
    17941 catctggagt tgctcttata caaaataata taaaagaaag acaatcttct gcattagcag
    18001 ttctaaccat ttttgcttca actacagcag gattaggtcc gacacttgga ggattattgt
    18061 tggacttagg aggatggcat gctatatttc ttgtaaatat acctgttgtt ttggtttcta
    18121 tatgcttagg gtatttttta ttcccaaaag aagtaaaaga aaagaaatct ataaaagaaa
    18181 ctttaaaaaa tgttttgtca agattagata ttattggcat acttctattt tcaattgcaa
    18241 tgatttttat attactattt ttgctatcta tgaaggaatc ttttaactta gaacaacttg
    18301 tatttggtat aatactgatg tgtcttttta tttggcatga attgagaaca aaattgccat
    18361 ttatagatat taagcttttt gtaagtaatc ctaaattgtc aaaagtttac ttgcaattta
    18421 ttatattaaa tttctttaat tatattttgt tttttggtct tcctagttat tttcaagatg
    18481 ccttacatta tagtgcaaag tcgactggat tatttatgct atttatgtct ggtataggta
    18541 tatttatatc tccattaact ggaaaatgga tagataaatc tggaactaga tttcctcttg
    18601 ttacatcatc aatctttatg tttattagtg cagtacttct ttcagtagtt tttatccata
    18661 tacctgtaat aggaaagggt attatattat cattagcagg tattagttat ggagttggta
    18721 atgtagccct gcaatcctct atgtttgaag aaagtcctcg tgacagtata gggacagctt
    18781 ctggtctgtt tcaaactagt cgttatttag gttctatttt agcaactgtt ttacttggaa
    18841 tggtatttga agtaaatata acatcagaac aatttcagat attaggttat gttatggtgg
    18901 tcttaggtac aataagtttt ttattaaatt ttatattaaa gaaagaatta agaaaagtag
    18961 aatagtggat ttatggattt aataagtcta ataactgata agtttaaaaa tattgtttgt
    19021 aaaacataac tggtttgata tttattaatt ctaagttttg aagcatagag tactcgataa
    19081 gtatacaata ttttgaatta aaattaagca gaaatttgaa ctagtctttc aaatctctgc
    19141 ttaatttagt agttaaattt ttttatagag aaaataagat ttgacactta gataaaaaat
    19201 cattgcattt tcaaaattct ttaaatcaaa cccaagtatc tcattaatct tttccaatct
    19261 atatactagc gtgtttctat gtaaaaacat actagtagat gtcttagaaa tatttaaatc
    19321 attatttata aattttattc ctgtata
//
LOCUS       DACULQ010000063        19286 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.063, whole genome shotgun sequence.
ACCESSION   DACULQ010000063 DACULQ010000000
VERSION     DACULQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.063"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1631
                     /locus_tag="JA154_08975"
     CDS             <1..1631
                     /locus_tag="JA154_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT7995997.1"
                     /translation="RMEILKYLLLNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIK
                     EYNLKLNKTLEGTKIIGKEVDIRKSIAKMMDDLLDENYEQHDISELTRLDSATFSALI
                     NLFDIDSIIFVETIITELEYNLGYTINQPYYINLITHILICLKRIEEGNQIESQEERE
                     ISVDNLDELVYKNVSTLIDKIENRYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDES
                     DRLSESEKVSSIMIENMSRFLNINLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLG
                     EIQERFSDVLGLCMMTINIMTEYDNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVC
                     HTGYGTSQLLAARLKREFPEWTIVDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIV
                     VSVLLMENDINNIKNALLSKPKKSMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLG
                     SKYESISLGKDLNIYIHKQSKLNKGIMNINNVTRTIDIYLEISNQSFLKNILMEISNL
                     YRQKNYINYIIDCKSKEDIKILFKNNLLEKTNGN"
     gene            1621..2070
                     /locus_tag="JA154_08980"
     CDS             1621..2070
                     /locus_tag="JA154_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT7995998.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNANTKKEILDIFYEV"
     gene            2170..3057
                     /locus_tag="JA154_08985"
     CDS             2170..3057
                     /locus_tag="JA154_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7995999.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            3460..4908
                     /locus_tag="JA154_08990"
     CDS             3460..4908
                     /locus_tag="JA154_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAT7996000.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMIIGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            4934..7324
                     /locus_tag="JA154_08995"
     CDS             4934..7324
                     /locus_tag="JA154_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAT7996001.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVEDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSVGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            7326..7997
                     /locus_tag="JA154_09000"
     CDS             7326..7997
                     /locus_tag="JA154_09000"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="catalyzes the interconversion of beta-D-glucose
                     6-phosphate to beta-D-glucose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAT7996002.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            8036..9406
                     /locus_tag="JA154_09005"
     CDS             8036..9406
                     /locus_tag="JA154_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAT7996003.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQDVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            9732..10595
                     /locus_tag="JA154_09010"
     CDS             9732..10595
                     /locus_tag="JA154_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator LicT"
                     /protein_id="HAT7996004.1"
                     /translation="MLKRRIIMIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGN
                     LIDKTKVNKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYI
                     SLSDHTYSAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEA
                     GFIAFHIVNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFF
                     AQRLLLDSAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTI
                     HLAKVVKDSNM"
     gene            10740..12668
                     /locus_tag="JA154_09015"
     CDS             10740..12668
                     /locus_tag="JA154_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit EIIBCA"
                     /protein_id="HAT7996005.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            12723..14156
                     /locus_tag="JA154_09020"
     CDS             12723..14156
                     /locus_tag="JA154_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAT7996006.1"
                     /translation="MTMSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRT
                     AVITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKG
                     DEETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEK
                     LCNVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVAS
                     AIATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYP
                     NYALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFT
                     SAKNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDD
                     YRIDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSG
                     NGSLRRMKKKSFEWYKKVIASNGEDLY"
     gene            14597..15379
                     /locus_tag="JA154_09025"
     CDS             14597..15379
                     /locus_tag="JA154_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HAT7996007.1"
                     /translation="MNMKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQD
                     CLMCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVS
                     SEIIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSK
                     EVEIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNS
                     PKLKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            15395..15760
                     /locus_tag="JA154_09030"
     CDS             15395..15760
                     /locus_tag="JA154_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAT7996008.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(15926..16675)
                     /locus_tag="JA154_09035"
     CDS             complement(15926..16675)
                     /locus_tag="JA154_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAT7996009.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            16847..17005
                     /locus_tag="JA154_09040"
     CDS             16847..17005
                     /locus_tag="JA154_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996010.1"
                     /translation="MYMRRDNNLLMKDYKYKGYFKGVEIFDNVNDLNLNTLTTVSRFV
                     LVFLFTIT"
     gene            17133..17558
                     /locus_tag="JA154_09045"
     CDS             17133..17558
                     /locus_tag="JA154_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAT7996011.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            17590..18777
                     /locus_tag="JA154_09050"
     CDS             17590..18777
                     /locus_tag="JA154_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAT7996012.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            18969..>19286
                     /locus_tag="JA154_09055"
     CDS             18969..>19286
                     /locus_tag="JA154_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996013.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDME"
BASE COUNT         7705 a         1978 c         3365 g         6238 t
ORIGIN      
        1 gaagaatgga gattttaaag tatttattac taaattcaaa taatataact tcaataaatc
       61 agttagcaga taaatattat gtaagtaaaa cttctatagt aaatgatttt aaatatatag
      121 aggcatggat aaaagaatac aatttaaaac ttaataaaac tttagaagga actaagatta
      181 taggtaaaga agtggatata aggaaatcga ttgccaaaat gatggatgat ttattagatg
      241 aaaattatga acaacatgat atatctgagt taacaagatt agattctgct acatttagtg
      301 cattgataaa tttatttgat attgattcta ttatatttgt agaaactata attacagaat
      361 tagaatacaa tttaggatat actataaatc agccctacta tataaattta attacccata
      421 tacttatatg tttaaagaga atcgaagaag gtaatcaaat agaatctcag gaagaaagag
      481 aaatttctgt agataatttg gacgagttag tatataaaaa tgtcagtaca cttatagata
      541 aaatagaaaa tagatacaga gtaaaaataa cgaaagatga aattatgtac atatatattt
      601 ttttggtatc atctggattt agtagcgaaa ataatagtag ttacaatgat gaatcagata
      661 gattaagtga aagtgaaaaa gtttcatcta taatgataga gaatatgtca cgatttttaa
      721 atataaatct caaaaatgat gacttactac agaaaagtct agcatctcat gtaaggccta
      781 tgttaaacag attaagatat gatatccaaa taaaaaaccc gttgttagga gagatacaag
      841 agcgattttc agatgtgctg ggattatgta tgatgactat aaatattatg acagagtatg
      901 ataatctgaa gaatattagt atagatgaag tttcgtatct tgccacatat tttcaagcgg
      961 caatagaaag aaatatgagt agtaaaagag taatagtagt ttgtcataca ggatatggaa
     1021 catctcagct ccttgcagcc agattaaaaa gagaatttcc tgaatggact atagttgata
     1081 tagttcctat gcatcagttg agtaagcgaa atttagatga tgtagacttt gtaatatcta
     1141 ctgtaaactt agatttgaaa gataaattac atatagttgt atcagtacta ttaatggaaa
     1201 atgatattaa taatataaaa aatgctttac tttctaaacc taaaaaaagt atgaacttag
     1261 atacaaactt attaggaatg tatttagaag ataatatata ttttaatttt gatgtagctc
     1321 agatggaaag tttaataggt gtgaatttag ggagtaaata tgaatctatt tcattaggaa
     1381 aagatttaaa tatatatata cataagcaaa gtaaattaaa taaagggata atgaatatca
     1441 ataatgttac tagaacgatt gatatttatt tagaaattag taatcaaagt tttttaaaga
     1501 atattttaat ggaaatatca aatttatata ggcaaaagaa ctatataaac tatataatag
     1561 attgcaagag caaagaggat attaaaatat tatttaagaa taatctattg gagaaaacaa
     1621 atggaaatta gagaaatatt aacagaagaa agcattattt tagatttgaa atcaactaca
     1681 aaagaaggca taatacagga attagtagat aaattatacg atacaggaag tataacttta
     1741 aaaagtaagt ttataaaggc attaaaacat agagaaaaaa taggctctac tagcataggt
     1801 tatcaaatgg ccatgccaca tggaaaaaca aaatatgtaa agaaggcatc catagtaata
     1861 ggtaaatcta aaaaaggtgt agattggcca agtatagatg gcttacctgt aaaggtaata
     1921 attatgtttg ctatgccaca atctggaggc aatgaagata tgatattaat atctaaaata
     1981 gcttacattt taggtggtaa aaatataggt gaaagacttt taaatgcaaa tacaaaaaaa
     2041 gaaatattag atatatttta tgaagtgtag gtattgtata aattataaat ttgaggtttt
     2101 taaaataaaa tgtgtattct tttaaatagt ataaattaat ttttagacac tagagaggag
     2161 aggatatata tggcaatata tttagaaatg cctaaattaa tacatgaatt tccttttcgt
     2221 agtctatcaa atgaaggaga agtattaaca acaccacact ggcataagga aatagaaata
     2281 ttacttataa cagaaggtgt agtaaatcta tttataaatg acaagcctat gcaattaaaa
     2341 aaaggcgaga tagtgataat aaaaggagga gatattcatt atatactgcc atcaccggga
     2401 agtgaaagat tagtatttca atttgatata tcttttttta atgatttaat tttattaaat
     2461 gaggaaaaag aaagcttaat taatttattc agtagtataa aaaaatgtag tagagattgg
     2521 tcagcaaaaa taagaaagtc agttttcgat atattgatag atatctacaa tgaagaccta
     2581 tacaaaatag aaggatatag ttatgcaata aaaggtaaaa tgtataattt aattcctata
     2641 ttatatagac agattcctag agaaattgaa gaaaatacag tagaatatga aataaactct
     2701 aaagagatat tagagagatt gaacaatata ttcaaatatg tagaaaaaaa ttataaacaa
     2761 cctataaaat tagaagatgt gtcttatgaa gtaggattta gtgtatatta ttttactaaa
     2821 tttttcaaaa aaaatacagg aaaaacattt ataacttttt taaatgaata tagattagaa
     2881 aaagcaaagt ggatattact taataacaat atatccattg tagatttggt tgaagaagtt
     2941 ggttttggaa gtacaaaaac tttttacaga gtatttaaag aaaaaattgg agtctctcca
     3001 ttagaattta aaaaaagata taatagcttg gaaaaaaaag ggagtgtctc aaaatgaact
     3061 tagttcatga ggcactttct tttatatgaa atcaaatata ttttcagtat ttcaacaatg
     3121 aaaattccat tcatttataa agtctaaatt ttagttctca atacaacctc tatttcgaaa
     3181 taaatccttt tatatataaa tatacagcaa tatatgagaa taagataaca ataatgagga
     3241 tataaaaatg tatttgctat atatataatt atatacatag atatctaata ttgatattta
     3301 atatgaaagt attgaaaata gacaatatta attatagtga gtaaaaaata taattggatt
     3361 tttaaattga attatttaaa aggtatagta tgaaaaaaat cttataacta aatatgagat
     3421 agaaattata aaactttata ttgtgaaaga ggtagatgca tgaaattagg gaaagatttt
     3481 ttatggggag gggcaatttc agccaatcaa agtgaaggat tttatttaga tggaggaaaa
     3541 ggtttatctc agtttgataa actcccatta aatgataaga gattaaaaga tgtatgttta
     3601 gatgaggata atattttaaa tgaaaataat caatactatc caagtcatat agggattaaa
     3661 ttttgtgaaa attacaaaga agatatagca atgttagcag aaataggttt taaatgtttt
     3721 agattttcta tatcttggtc aagaatattc ccaacaggag aagaaagtga accaaatgaa
     3781 aagggattac aactttatga taatataata aaagaattaa aaaaatataa tatagagcca
     3841 ttaataacga taagccactt tgatatacct tattctctag ttgaaaaata ttctgggtgg
     3901 gctgatagaa gagtggttaa tttatatgta aaatatgctg aaattttgat gaaaagatat
     3961 aaagatgatg tcaaatactg gattcctttt aacgaaatga atatgataat gcatattcca
     4021 tatataggtg ctgggttaac ttttatagct gatgaaaatc aacttcagaa aaaatatcaa
     4081 gcagcacacc atcaattatt agctaatgca aaaactatag aaataggaag gtatataaat
     4141 aaagaattta agtttgggtg tatgttagct gctggaaaaa catatccata cacatgtaaa
     4201 ccagaagatg tatttgcaac tttagagcat gaaaggcata atttgttttt ttctgatgtt
     4261 caagttaggg gtgtgtatcc aagctttatg aattattatt taaaatcaaa tcaaataaaa
     4321 ttaataataa aagaggaaga tttatcaaca cttaaggata atactgtaga ttttgtatct
     4381 tttagctatt attctagtgc ttgtagttca gctagagctg aaggtttaga aaaatcaaag
     4441 gcaaacggac cagaaaccat aaaaaatcca tatcttccaa aatcaaatag tgtatggcaa
     4501 aacgaccctg tagggttaag aataactatg aatcagttat atgaaaggta tcagttacct
     4561 ttatttatag tagaaaatgg actgggtact caagatattg taaatgaaga tggaaaaata
     4621 attgatgatt atcgtataga ttacatgaaa gaacatatta aaaatatgat tattggagta
     4681 aatgaagatg gaatagagct tataggatat cttatgtggg gatgtataga tgtaaccagt
     4741 gtaagtgaag gaaagatgac aaaacgatat ggcataattc atgttgattc tgatgactat
     4801 ggaagaggct catttaaaag aactaaaaaa caaagtgctt tttggtataa aaaagtaata
     4861 gaaacagatg gagaaatttt atttaatgac atggaaactc atgcataatt cattttaaga
     4921 aaggagtgtt attatgagtt taatatttac tactaaggac caacttatag agcaagggtt
     4981 tagtaaggat acaataaaat ccaatgaaac gctgtttaca ctagctaatg gacatttagg
     5041 agttagaggg aatattgaag agtctgattt taataatgag tatgtagata atatgggtac
     5101 atatgtaaat ggattttatg aagaatctcc aattacatat ggagaaagcg cttatggcta
     5161 tgcaaaacaa aatcagacta tctgtaagtt acctaatgct catatagtaa atttctctat
     5221 tgaaggagat tgctttgatt tatcaaaagg tattacaagt gaacattcta ggatactaga
     5281 tttaaatgaa gggattctta aacgttcatt tatatgggaa aactctcgtg gtagaaaagt
     5341 taaagtatct atagaaagat tagtatcaca agatatagaa gaattaatga tgatttcata
     5401 tacaataaca ccattgaatt ttaatggaaa aataaaattt aaaaataaaa tggaaaatag
     5461 tatggagaag tttaattgtg aaaaagtaga ggacccaaga gtggcatcta taaataagaa
     5521 gcaatttaaa attaatgtaa aaaaatataa caataagtat tttatgaatt tagttacaga
     5581 aagaagtaga ttagggttat ggtgtgggtt tgattcatat attacttgtg gaaatattac
     5641 aaatgagacc tataatgttt cagatgaatg tattgagcga gaaatagaag tctatgccaa
     5701 aaaagatgaa aggttaaatt tagaaattgt tgtaggctat agtagagtat attctgattt
     5761 aaatctaaat gaagaacgtt taaaatcatt agaatgtctg gagtcgttaa aatgtttaga
     5821 atcagaagca catttaaaat cattagaatt ggttttaaga gaagcatgta atattggatt
     5881 tgataaagtc aaaaatattc agagaaagaa aatgaatgag ttttggacaa cctctgatgt
     5941 agagattgaa ggcgataaaa ctcttcaact ggggataaaa accaatttat ttcatattta
     6001 tcaatcagta ggaagagatg gaaagactaa tatatcagca aaaggtctta caggtgatgg
     6061 ctatgagggg cattatttct gggatacaga aatgtatata ttaccatttt ttatatatac
     6121 tcaaccatcc attgccagag ctttattaga ttatagatac tcaatcttag atgcatcaag
     6181 agaaagagct agagaacttg gaataaataa aggttgctta tattcatgga gaactataaa
     6241 tggtaatgaa tgcagtgcat attatccagc aggaacagca cagtttcata taaatgcaga
     6301 tatagcttat ggtattagaa cgtattttga agcaactaat gatgaagaat ttatgatgca
     6361 aaatggctta gaaattctta tagaaacagc aagattttgg gttgaatttg gagattatat
     6421 agaatcaaaa aataataaat tctgtataaa tggagtaaca ggaccagatg aatatacagc
     6481 aatagtaaac aataattact acactaattt aatggctaag tataatttaa tatatgcagt
     6541 acagatggta gataaattta agcataattt attttcaaat gaagtattta gaaaagttaa
     6601 attacaagaa tatgaagtga aaaactggat aaatgccgct caaaatatgt atttaccata
     6661 tgatgaagag aagaaaataa gcaaacaaga cgattcattt ttagaaaaat cagtatggga
     6721 ttttaaaaat acaccaaaag aaaaatatcc attacttctt cattatcatc cattaacaat
     6781 atatagatat caagtaaata aacaagcaga tactgtactt tcagcgttgc ttttccctga
     6841 tgagtttagc ttagaacaaa agaagagaga ttttgaatac tatgaaaaaa taactactca
     6901 tgattcatct ttgtcaaggt caatatttgg aatgatggca agtgaaattg gaaatcatga
     6961 aaaagcttat aactatttta tggacacagc attaatggat attaataacc tacaaaaaaa
     7021 tacaagtgat ggaatacatg ctgctaatat gggtggaaca tggatgagtt tagtatatgg
     7081 atttgcagga atgaaaataa aaaatgataa attaagtttt gaaccaagga tacctaaaca
     7141 ctggaaaaat attaaattta atatattatt tagagataat ttagtttctg taaatataga
     7201 agaatataaa tcttattata agttaatcaa aggtagaaat ataaccatat tacatgataa
     7261 taaagaaata aaattatcaa agaacataat agaagttaat aattgtttta aagggagtaa
     7321 ttaatatgaa agcagtctta tttgatcttg atggagttat aacagatact gctaagtatc
     7381 attttgaagc ttggcatgag atagcaaaat cgctagatat agaaatagat gaaactttta
     7441 atgaaaaatt aaaaggcata agcagggaag aatcattaga taaaatattg ttatctgcta
     7501 ataaagtaga tagttatact aaagaggaaa agttaaaggt gtgtgaagaa aaaaataaca
     7561 tttatataaa gttaataaac tcattaacat ataaagatat attaccaggg ataaatgagc
     7621 ttataaaaga acttaaaaaa aataatgtga aattgggaat agcatctgct agtaagaatg
     7681 cacctaggat attagaatct ttgggaataa aagatgaatt tgattgtata gtaaatccat
     7741 ctttattaaa aaggggaaaa ccatatcctg atatttttat tgaagggagc aagattctaa
     7801 atttaaaaca atatgattgt gtaggaatag aagattctta ttcaggtatt aaagctataa
     7861 atgctgctaa tatgacatca attggtgtgg ggaaaaaaga agtactagga aaatgtgacc
     7921 atattgtaga agatactagc ttattaaatt acaatacact attacaagct tggagtaaaa
     7981 agattaattt ggattaaaat tatattaaaa attattttaa aagagaggat aaggtatgaa
     8041 gaataaatat gaagaagtag ctcaattgat aattgatgga cttggaggaa aggataatat
     8101 aattgactta actcattgta tgacacgatt aaggttcatc ttaaaagatg aaaagaaagt
     8161 caatgcagat aaattaaaat ctatagataa ggtagttgga gtaaactcaa cttcaactca
     8221 atatcaggtc ataataggta atgaggtagg tgttgtattt aatgcaataa taaataaggg
     8281 gataaatacc acaggagata ttaaaagcga tataaaaaat aaaaataaaa aaaatagtat
     8341 tttaaataat ataatagata caataactgg ttgtatgaca cctatgatac cagcccttac
     8401 agcatctggg atgataaaag ttatattatc tctttgcacc acttttaatt ggctatcatc
     8461 aaattcaagt acatatagag tattagatat aataggagat gtagcatttt actttatgcc
     8521 tataatactt gcaataaatg cagctaaaaa atttaatgtt aatacatcaa tagcagttat
     8581 agtagtaggt gtgtttttac atccaaattt ctctgcatgg gtatcaagtg gggaccctat
     8641 atcatttata ggagtaccaa ttcaagatgt tatatatgca gcatctgtta tccctgcact
     8701 tttaacagta tggatgatgt catatataga aaaattcatt gataaattaa cacctagcat
     8761 gttaaaaaca attttaaatc caactttagt attattaata agtgcgccaa tagcattaat
     8821 agtaattgga ccaataggaa atttcttagg agagggatta gctagtataa taaatttact
     8881 acaaggaagg ttaggattta taatggtttg tttattagca gctgcaatgc catttatcgt
     8941 tatgacaggg atgcatcatg cattgactcc tatattcata tcagcatttg caactacagg
     9001 acaagaatcg ctaatattaa tatctcaagt ttgtgctaat ttagcgcaag gtggcgcaac
     9061 attagctgta gcattaagaa gcaaagatag taagatgaaa cagttagcat cagctgcatg
     9121 gatatcagct atcatgggta taactgaacc agctctttat ggagttacat tgaaacttaa
     9181 aaaacctgct atgttagcat ctataggagc aggaatcgca gggttatttg caggaattac
     9241 tcatgtgact ttatatgttc cacaaaatag tataatggca gtacttggat tatcagggga
     9301 aaaggggatt tcaaatatag taaatggact tatagttata tttatagcat tggttgctcc
     9361 atttattatg acatatgttt ttggagttga aaaaaatgtt aaataaagta cagataatgt
     9421 agtagaggaa gtataatatt aactatttac attaatagaa tttttattat tattgactat
     9481 aacttgtttt gtatttatat tgatgcaaag aaataagtta atattaataa gaataattat
     9541 tagaactgac aaaaagcaac ataaaagatt gatgaagtat atggaaataa aaagttgaca
     9601 aaatatgtct gatgttttat catattaatt ggaattaggt agtaattgtt taagtacaag
     9661 ctaagcttat tcattaataa tagcagtatc ctaggaagga gtatgcccat tattatggat
     9721 aagctttttt tatgcttaaa aggaggataa tcatgataat taataagata ttaaacaata
     9781 atgttgtaat cacattagat gataatgatg aagaagttat cgttatgggt aaaggaattg
     9841 gttatcaaaa atcaaaaggc aatctaatag ataaaactaa agtaaataaa gtatttagaa
     9901 tatcaaataa agaaatatca aacaaactac aagaattact caataatatt cctatagaac
     9961 acatgaaatt atcaagtgaa attatagaat acgcacaaat aaagttaaac aagaagctaa
    10021 atgaaagtat ttacatatcc ttaagtgacc atacatattc tgcaattcaa aggatgaaag
    10081 aaggaataaa tgtaaaaaat gcaatccttt gggaaattaa aagattttat aaagaagagt
    10141 ttgaaattgg aatgaaagca ctagatatta ttgaaaacaa aacaggtata aaattaccag
    10201 aagatgaggc tggatttata gcctttcata tagtgaatgc tcagttaagt gaaggtcata
    10261 ctttggcatc tgatattacg aagttaatac aagaagtact tagcatagtt aggtatcatt
    10321 ttaggataga atttcatgaa gaatcagtgt tttattatag atttataatg catttaaagt
    10381 tttttgcgca aagattgctt ttagatagtg cacatgaagg tgagacagac aaagaattac
    10441 taagcattat taaatctaaa tataataaag agtttgaatg tgtagttaaa attaaaaatt
    10501 tcattaaaaa acaatataac tacatattaa cagatgatga gcttatatat ttaacaatac
    10561 atttagctaa agtagttaaa gactcaaata tgtaagtgaa ataatttttc agtagtatat
    10621 taagaatgat tgaaaggagt gatactaaca aaattatact tatggttgga tggtaacatt
    10681 gagttggcca aaccttcaaa tttcaaaaaa ttaatttaaa cgaaatatgg aggagaaaaa
    10741 tgggaaaata caaacaatta gcacaagaga tagtaaacaa tgttggtgga aaagaaaata
    10801 ttagtgggtt agtacactgt attacaagat tacgttttac tttaaaagat gaaagtatag
    10861 caaatgacaa tgtattaaaa aacatggaag gtattgttac tgtaatgaaa agtggaggac
    10921 aatatcaagt agttatagga aatcatgttg aagctgtcta taaagatgta gttgaaataa
    10981 ttggtttaga tgattcaagt acaagttcag aaactaaaaa atcaggtaat atactagata
    11041 aaggtataga tataatatca ggaatttttc agccagtgtt aggaataatg gcagcttgtg
    11101 gtatgttgaa aggatttaat gcattatttg tagcaatggg attatatagt gatgtatctg
    11161 gtggttatat ggtaataaat gcagcaggag atgcattatt tacattcctt cctttgttct
    11221 taggatatac ttcagctaag aagtttggat taaaaccaat gttgggatta gcactaggag
    11281 ctgcagtatg ttatcctgct atacaaggta gcagtatatc agcaggagca gatgtgatgt
    11341 acactttatt taaaggaaca atgtttgcat caccagtata tattgatttc tttggactac
    11401 caattgtatc aattgattat actggaacag tcgtaccaat tatatttata gtgtattttg
    11461 cttctaggtg tgaaaaatta tttaataagt gtgttccaaa tttagttaag tttttctttg
    11521 tgccaatgct tacattatta ataacagtgc ctgttgcact tatagttata ggccctatag
    11581 caacatttgg ttctacactt atttcacaaa ttgtaatttc aataagagat tttagtccac
    11641 tattggcagg tgctatagta ggatttactt ggcaaattct tgttattttt ggtatgcact
    11701 ggggatttat tcctgtatat ataaacaatg ttatgacttt aggatatgat aatgttatga
    11761 tgccattctt tgcctgtaca tttgcaagtt cggcagttgt gttggcaata tttttcaaga
    11821 ctaaagataa aaaactaaaa gagatggcaa taccaaactt tatatctggt atatttggag
    11881 ttacagagcc agcaatttat ggaatattat tgcctttaaa gaaaccattt ataataagtt
    11941 gtattgctag tggtattgga ggcgctttct atggattctt taatcttaga aaatttataa
    12001 ctggaggaat gggaatattt gaacttccag caatgattga accagatggg ggaatgggta
    12061 acttaatagt agctctttct ggtatagcta tatcaatggt cgtagctttt gtattaacta
    12121 tgattttata taaagatgag aaagtagaag agtctaaaaa agtaagaaat aagggtaaag
    12181 aagaaataaa agaagtcaag tctactaaat tagaaagaga aatagtaaca agtcctataa
    12241 aaggtgaagt attaaaatta agtgatatag aggatgcagc atttgcatct ggtgtactag
    12301 gtcaaggggt ggcaataata cctagtgatg gaaaagtagt tgctccagta gatggggtag
    12361 ttacaacctt atttcctagt ctacatgcta tagggattct ttctgataca ggtgttgaag
    12421 tgcttattca tattggatta aatactattc aattagaggg aagaggtttt gaggcatgta
    12481 taaaacaagg tgatagaatt acaaaaggtc aaactatatt aaactttgat gtttatgcaa
    12541 taaaagagtt ggggtatagt actgtaactc caattgttat tacaaattca tcacaatttt
    12601 tagatgtagt agaaacagaa agtaaaaata ttgaattaga agacaatcta attacagtct
    12661 tattttagta aagattgagg tattagcata atctagtgct ttggttataa ttttaggagg
    12721 ctttgactat gagttttaag aatgattttt tatggggagg agctactgcg gccaatcaat
    12781 gtgaaggtgg atacaatgaa gacgaaagag gattggcaaa tgtagatgta tgtccaacag
    12841 gaaaagatag aacagcagtt attacaggga aactaaaaat gtttgatttt gatgatgagc
    12901 attattatcc agctaaaact ggtattgata tgtatcataa ttataaagaa gatataaagt
    12961 tatttgctga aatgggattt aaagtatata gaatgagcat tgcatggagt cgaatatttc
    13021 caaaaggtga tgaagaaaca ccaaatgaaa agggacttca attttatgaa aatatattta
    13081 aggagtgtaa aaaatataaa atagagcctt tagtcactat tacacacttt gattgtccaa
    13141 tgcatttagt taaaaagtat ggtgcatggc gtaatcgaaa aatgataggt ttctatgaga
    13201 aattatgcaa tgttatcttt agaagatata aaggattagt gaagtactgg ctaactttca
    13261 atgaaataaa tatgatttta catgctccat ttatgggtgc tggtatttgc tttgaagaag
    13321 gtgaagatgt tgaaaagata aaatatcaag ctgcccatca tgagcttgta gctagtgcaa
    13381 ttgcaactaa aatagcacat gaagttgatt caaataactt aattggatgt atgtttgcag
    13441 ctggaagtgt atatccttat agttgtaatc ctaatgatgt atgggaagct acaaagttag
    13501 atagagaaaa ttacttcttt gtggatgtac aatctaaagg taaatatcct aactatgcgt
    13561 taaagtatat ggagcaaaaa ggtattacac cagaaatgga acctggtgat attgaattat
    13621 tgaataaata tacagtagat tttatttcat tttcatatta caatagccgt tgtgttagaa
    13681 ccgatgaaaa tgcagatgat atggctgaag gaaacatatt tacatctgct aaaaatccat
    13741 atctttcata cagtcaatgg ggatggccaa ttgacccatt aggtttgaga attacactaa
    13801 atcatgtata tgatagatat gaaaagccac tatttattgt agaaaatgga ttaggtgcta
    13861 aagatatagc tgatgaaaat ggttatgtag aagatgatta cagaattgat tatctaagag
    13921 aacatataaa agctatgcat gatgcagttt ctatagatgg ggtcgattta ttaggttata
    13981 ctacatgggc acctattgat ttagtaagtg ctggtacagg tgaaatggaa aagcgttatg
    14041 gatttatata tgtagataga gacaatagtg gtaatggaag tctaagaaga atgaaaaaga
    14101 aaagttttga atggtataag aaagtaattg cttcaaatgg tgaagattta tattagacaa
    14161 gaaatctatg ttaaataaga aatctatatt agacaagaaa tctatgttaa ataagaactt
    14221 tgtattaaat aagaagagta taaaaattaa taataagtat aatttttaac ttttttaact
    14281 atagaaaatc aatatagaat tattttacat tgtattgatg caatagtatg attcacaaat
    14341 aaaattttag aaaataaaat tgttaagaag tatgggaagt aagtgcaatt tactttgtaa
    14401 taaaagatga aaaaggcaag cttaggtttt aagcttgtct tttttattat gtataaacta
    14461 tgatatttta aaattctatg aaaaatttat ttaatagaga aattaaaaat ttaatggtaa
    14521 tcaaattctt taccaatgaa tataaaaaat gtctacgtgt tataatagtt taaactaata
    14581 aacgaaaggt agatgaatga atatgaaata ccaacatata atagaagttg acaaagaact
    14641 ttgcattgga tgtgggcttt gtaaaaatga ttgtccagta aacaatataa ttatagaaaa
    14701 taaaaagtca gtaattaaaa aacaagattg tctaatgtgt ggacattgtg cagcaatatg
    14761 tcccacaaaa gctattacct tgacaggatt tgatgaacct cctatagaac ttacaaataa
    14821 gccaaagcta gattcagatg aattactaat ggcaattaaa tctagaagaa gtatacgtaa
    14881 atttaaagat aaagaagttt cttcagaaat tataaagcaa attattgagg caggtaggta
    14941 tacccctagt gctaaaaatt cacaagatgt atcatatatt gttttagaca ataaaaagtc
    15001 aatttatgaa aacgaagcag tgaaattttt tagaaaaata aaacctattg caaatatagc
    15061 tattaaatat tcaaaagaag ttgagattga cgataacttt ttctttaagc atgctcctat
    15121 tgcaataatg ataattacaa aagataagat tagtggttca cttgcagcat caaatatggc
    15181 attaatggca gagtcttatg gtctaggtgt tttatttagt ggatattttt cagatgtagc
    15241 aaataattcg ccaaaattaa aaaagctttt aggtctaaaa cgtagtaatc atgttcttac
    15301 aacattagta attggatacc cagatgttaa atatagacgt actgcacaga aagaagtagc
    15361 aactgttaga tacttataag agagggtgga ttttatggaa aaagaatgtc aacaaagaat
    15421 gcaagaaata acgtctaaat ttaaagaatg tcgcaaagct atctctgcta tgggtgatga
    15481 gactagacaa ttgattttaa ttgctctttt agagagtgat tttaatggta tacgagtggg
    15541 agaaatcaca aaaaaaacac atctttctcg tcctgctgta tctcatcatc ttaagatatt
    15601 aaaagaagca gagattgtta atttgcgtag agaaggaacg aaaaattatt attatcttga
    15661 ttcaaaaaaa tcacaatgga agtcactaac agagctaata aatcttatat ttgttggaat
    15721 acaacatatt agtgaaaatg atataagaaa aggtgattaa attatacttg atttaataca
    15781 aaaagattga tttaggaact aaaaaattca ctttgagaca ctatcttttg aactataccc
    15841 attttaggag acggttaaaa tatgtaaaac tgtataaaac aatgggtata gttcatttta
    15901 taatggttta aatattaaga atatattatt ctaaatagta tctttctctg ttttgagttt
    15961 tatttttata ctcaatagct tcagtggggc aaccacaaat acatgccata caatgagtac
    16021 agttattttt ccaaactggt tttttgtttt ttaaattaat attatttaaa ggacatagct
    16081 ctacacattt tccacagcca atgcatttat cagtataatg aaatcctttg gcactaataa
    16141 ttgtcttata aaaaactgta tttattatac cacttttaat tgtacctcct aagccactag
    16201 gagtaatagt ttcaaattca ttttcattgc gtatatcaga aataatccta tatatttgtt
    16261 tatctgcttc tttaatcatt tgttttgatg tttctttgtc tgttgtagaa aataaagcta
    16321 tataattctc tggcatttta atttctgcca taccttttaa ttgccattcc ttgtatttac
    16381 atagtttttg aatatagttt atggattttg ctgtatctcc accacaagtc attacaaagt
    16441 aaactctgtt gcttcctaga aattttgttt cttttataaa atccgtaact actattggca
    16501 atctccatgc ataagtagga catacaaata tgaatggttt atctgctgat attaattcat
    16561 ctgttttttc ctcttttata agttgattta gtgatatcag ttcatcattt aattcttgtg
    16621 caattttacg agccacatat cggctattac cagttccgct aaaatataaa atcataataa
    16681 tatctcccta actatttttt atttattata gtaatttatt gatatattct attcaaatgt
    16741 atgtaaaatt tagtagttaa gaaaattata acacattagt aatttttata cttattggta
    16801 aagaatttga ttaccattaa atttagcaac aagacatcaa ctggacttgt atatgagaag
    16861 ggataataat ttgcttatga aagattataa atataaaggt tattttaaag gtgtagaaat
    16921 atttgataat gtaaatgacc ttaacttaaa tactttaaca acagttagta gatttgtgct
    16981 tgtttttttg tttacgataa cttaaggaaa tatgttacct gaataataaa tttatataat
    17041 aaaagtatat aaatatatat atgaatgtta agattagttg atagaattta aataataaat
    17101 ccaatattat aaacatagga ggtatgaaaa atatgattaa tgagaattta aaaagtctaa
    17161 gaaaaatcaa ccagtataca caagaagaat tagctgaaaa acttaatgtt tctagacaat
    17221 caatagcaaa atgggaaagt ggagaatcta ttcctgatat atgtagttgc attaaacttg
    17281 caaaattgta caatgtcaaa ctagatgact tagttaatca ttcagaagaa aaaacaggta
    17341 ttatagtacc tcctaaagga aagtttttct ttggagcagt tgtagttggt gaacgtggtc
    17401 agattgtcat tccaaaagaa gcaagagaag tatttaatat aaatgcagga gataaattat
    17461 tggtacttgg agatgaggaa agaggattag gaattgttca tcaacgtgat ttgataaatt
    17521 ttataggtca tgtgggagta gtacgtaaag aagaatgatt taaaaaaatt ataaaaggag
    17581 gtatgaaata tgagtaaaat agtatttttt agcattcctg catatggtca tactaatcct
    17641 actattgaag tagttcgtga attagtggat agaggaaatg aggtattgta ttattctttt
    17701 aatgaattta aagataaaat tgaaggtgct ggagcaaaat ttatttgttg tgataaatac
    17761 cttccagaat tactccctgg agatgagaaa aaaattggta aggattttcc tggattaatt
    17821 gagatgatag tagatactac tatttcttta gatgaaaaag tttgtagaga acttaaagat
    17881 tttaatccag attgcattgt atcagattca ttaagttttt ggggaaagtt atttgcacaa
    17941 aaacttaata ttccatatgt atgctctaca acaacttttg catttaataa acatacagca
    18001 aagttgatga aacaaaactt tatagaaatt atacgtatga tttttggtgt aagacgcatt
    18061 aataaaaaaa taaagctttt acaaagaaaa gggtatgaag taaaaaattt tatttctata
    18121 gtttctaatg ataatgatac ggataccatt gtatatacat caaaagaatt tcaaccaatg
    18181 gtagaaacct tctctagtaa gtattcattt gtaggtccat cagtatcaaa gttgattatt
    18241 gaacctaaag agagaaaaag aaagctaata tatatttcgc ttggtacaat taataataaa
    18301 aatgttagct tttataaaaa ctgtataagt gcatttaaag attctgatgt tgatgtgatt
    18361 atgtctgttg gtagaagtac tgatattaaa agtttaggaa atataccaaa taattttgag
    18421 gtcaaaaata gtgttgaaca gattgctata cttcaaaaaa ctgatgtgtt tgttactcat
    18481 tctgggatga atagtgttaa tgaaagttta tattatggag tacctatggt attatttcct
    18541 caacattcgg agcagagaat ggtagctaag agagttgttc atttgggagc aggaattatg
    18601 ttaaaagagg ataagtcaga aagtattaaa aaagcagtat ttcaagttat aaatgacaat
    18661 gaatataaag aaaatgcaac taaactatca aaaagctttt ataatgcagg aggctcaaaa
    18721 aaagcagcag atgtaatatt acagattata tgtaatagta gacaagttga catataatag
    18781 atttgttaaa aatatatata catacatgta atttaatcaa agtactgaca gtggggtctt
    18841 ttaacctacc agttggggca tatatattga tgctaaatat gagggatgtt atattttaat
    18901 aaaaaagata acatcctttt tttagtgcat tatatcgagt tttaaataat aaatgaagga
    18961 ggattaatat gaaaaaatat aaagttattt ttatagtatt aattataggt attataagtg
    19021 taacagggtt tgttgtatat aaaaactgca ttaaaccatg gaaagatgct gaaacatata
    19081 ttgataagta tatggtaaaa caagatattt caaaagataa tataaaaagt attacaaaag
    19141 aaaaagccaa gaaggcaagt tacgaaggaa tactatacaa agtgtattac aaagatgacc
    19201 ctcgatatga gtatcaatat ttttatagtg ataattatta tgctctatat gtaaataaag
    19261 taatgttaca aatttatgat atggaa
//
LOCUS       DACULQ010000064        19275 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.064, whole genome shotgun sequence.
ACCESSION   DACULQ010000064 DACULQ010000000
VERSION     DACULQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.064"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            16..666
                     /locus_tag="JA154_09060"
     CDS             16..666
                     /locus_tag="JA154_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7996014.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(823..1854)
                     /locus_tag="JA154_09065"
     CDS             complement(823..1854)
                     /locus_tag="JA154_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAT7996015.1"
                     /translation="MVLNMEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKI
                     NRTKENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEG
                     QVIVNVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDL
                     KIAQMIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMT
                     RGISEMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLK
                     EVKMVVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEM
                     EEVVLGDDI"
     gene            complement(1873..2487)
                     /locus_tag="JA154_09070"
     CDS             complement(1873..2487)
                     /locus_tag="JA154_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-phosphate glycerol 3-phosphate
                     acyltransferase"
                     /protein_id="HAT7996016.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(2522..3847)
                     /locus_tag="JA154_09075"
     CDS             complement(2522..3847)
                     /locus_tag="JA154_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAT7996017.1"
                     /translation="MSISRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRI
                     FAEVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAA
                     DKEIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLD
                     EIVENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAID
                     TYFEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTE
                     QDTKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAK
                     TNQRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTD
                     IRPPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(3875..5212)
                     /locus_tag="JA154_09080"
     CDS             complement(3875..5212)
                     /locus_tag="JA154_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HAT7996018.1"
                     /translation="MKILEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIH
                     DIIEYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCM
                     FCFIDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVH
                     TTNPELRQKMISNKFAGKLYGIMKRLADTHIEMNCQIVLCPGINDGKELDRTIKELAQ
                     LYPYVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFL
                     SDEFYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSI
                     ATGHSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDK
                     ELGEALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(5441..5641)
                     /locus_tag="JA154_09085"
     CDS             complement(5441..5641)
                     /locus_tag="JA154_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996019.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(5643..6575)
                     /locus_tag="JA154_09090"
     CDS             complement(5643..6575)
                     /locus_tag="JA154_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore envelope protein"
                     /protein_id="HAT7996020.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(6620..7552)
                     /locus_tag="JA154_09095"
     CDS             complement(6620..7552)
                     /locus_tag="JA154_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996021.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(7607..8896)
                     /locus_tag="JA154_09100"
     CDS             complement(7607..8896)
                     /locus_tag="JA154_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT7996022.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(8925..9617)
                     /locus_tag="JA154_09105"
     CDS             complement(8925..9617)
                     /locus_tag="JA154_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996023.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(9632..10393)
                     /locus_tag="JA154_09110"
     CDS             complement(9632..10393)
                     /locus_tag="JA154_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAT7996024.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(10390..10851)
                     /locus_tag="JA154_09115"
     CDS             complement(10390..10851)
                     /locus_tag="JA154_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAT7996025.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(10873..11136)
                     /locus_tag="JA154_09120"
     CDS             complement(10873..11136)
                     /locus_tag="JA154_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HAT7996026.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(11240..12013)
                     /locus_tag="JA154_09125"
     CDS             complement(11240..12013)
                     /locus_tag="JA154_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089153.1"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; this sigma factor is
                     responsible for the expression of sporulation specific
                     genes and is expressed after engulfment; this factor is
                     involved in the transcription of small acid-soluble
                     proteins involved in protecting the forespore chromatin;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-G factor"
                     /protein_id="HAT7996027.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(12105..12827)
                     /locus_tag="JA154_09130"
     CDS             complement(12105..12827)
                     /locus_tag="JA154_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089154.1"
                     /note="sigma-29; sigma factors are initiation factors that
                     promote the attachment of RNA polymerase to specific
                     initiation sites and are then released; this sigma factor
                     is responsible for the expression of sporulation specific
                     genes and is expressed in the mother cell at the onset of
                     sporulation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HAT7996028.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(12852..13697)
                     /locus_tag="JA154_09135"
     CDS             complement(12852..13697)
                     /locus_tag="JA154_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAT7996029.1"
                     /translation="MVYIEYYVIENLVINYIIISCTSILTKRYNPKKNKLLGASLGGA
                     YSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLVNIFISGSTY
                     FIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTININLLEKSFCC
                     KALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQEIIGSLDDK
                     TSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDREYGAIMNPN
                     ILPGF"
     gene            complement(14014..15288)
                     /locus_tag="JA154_09140"
     CDS             complement(14014..15288)
                     /locus_tag="JA154_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAT7996030.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(15572..16732)
                     /locus_tag="JA154_09145"
     CDS             complement(15572..16732)
                     /locus_tag="JA154_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAT7996031.1"
                     /translation="MMLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISV
                     NTDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFV
                     TAGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVD
                     TLITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTI
                     MKDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEV
                     NEASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSI
                     RSSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(16919..17272)
                     /locus_tag="JA154_09150"
     CDS             complement(16919..17272)
                     /locus_tag="JA154_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HAT7996032.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(17371..18045)
                     /locus_tag="JA154_09155"
     CDS             complement(17371..18045)
                     /locus_tag="JA154_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HAT7996033.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(18050..18748)
                     /locus_tag="JA154_09160"
     CDS             complement(18050..18748)
                     /locus_tag="JA154_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HAT7996034.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIEIYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(18771..>19275)
                     /locus_tag="JA154_09165"
     CDS             complement(18771..>19275)
                     /locus_tag="JA154_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAT7996035.1"
                     /translation="NPYIENVEIKRKLPNKIIISLKEKEIFAVLKDEDNYCYIDKKGN
                     LLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTKENVFKTLNYLKEEGIYRKINY
                     VNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKILIDLQNKNTNGGTINLNYGKL
                     AVYSPEG"
BASE COUNT         5884 a         3578 c         1966 g         7847 t
ORIGIN      
        1 ttgtaggtga ataaaatgac aaataaagaa ttacaaaaga aaaggatatt gatgtatttc
       61 atagaagctg ctcaaaatat tatggaaaac gaaggtatag aaaatattac acttcgtaag
      121 gttgcagata tggctggcta taatagttca actctatata aatactttaa aaatttagac
      181 catttaatat catttgcatc tataaaatat tttaaagatt ataacttgaa tatttcaaga
      241 tgtatagaaa atgtcaatga tgaatataag aaatatataa ttatgtggaa attattttgt
      301 aagcattctt ttgataatgc tcaagctttt taccaggtat tttttaactt gtctagtgat
      361 gaacttagct atataacaaa acaatattat gatatgtttc cagaagattt aggtattcat
      421 gatagtgaca tatctttaat gattacagga gaaagtataa aggaaagaaa taagatacta
      481 ttaaacaatc ttgtagatac tggatatata ccagctaaag atttagacat aatcaatgat
      541 ataattattt ctttttatca aaatctatta ttcttaaaaa agaacacagg aaaaaattct
      601 gatgatgatg aattaactat gaaaatgatt tcttctattg aatacataat aaaacatgct
      661 aaataaaatt aaaatatggc tgtctcaaaa tagagataag ccttttttat tgttgaaata
      721 ataaaaatat atttaatttc atataaaaat gagtgcctcg taaactaaaa agttcatttt
      781 gagacacccc taaaataaat ttattattta ttatacaata gtttatatat catcacctaa
      841 aactacttct tccatttcat gctttttact tctcatcatt agttctattc caacttcttt
      901 aggatttgaa cctttcttta ttacagaata tatagcatta gttataggca tatctatatc
      961 taatttttca gcaacatcat gtgctacttc agtagcagtt atgccttcta caaccatctt
     1021 tacttctttt aaagtatctt ctaaactcat ccctttacct atgagtatac cagctcttct
     1081 atttctacta tgcatacttg tacaagtaac tattaaatct ccaatacctg aaagacctgc
     1141 aaaggtagac atattagctc ccatagcaat tccaagtctg ctcatttcac taatacctct
     1201 tgtcataagg gcagcttttg cattatcacc ataccctaat ccgtcacata tcccagctcc
     1261 aaatgctata atatttttta atgctcctcc aagttctact ccaactatgt ctggattcgt
     1321 ataaactcta agtttagggc tcataaacaa atcttgtatc atctgtgcta tttttaaatc
     1381 tttagaagca actactacgg tagtaggaat atcccttgct acttcttcag catgagatgg
     1441 cccagatagt attacatatg gattttgtgg taattcttct tcacagactt gtgataatct
     1501 aagcccagtc cctttttcaa gacctttagc tacattaaca ataacttgtc cttcttttat
     1561 aaatggtttt attttttggc aagtacttct tacagcctgt gatggaactg ctaaaactat
     1621 taccttacaa tcttttatag ttttttctat atcagtactt atagttatat tatttggaaa
     1681 taatactcct ggtaaatagt ctgtattttc tttagttctg tttatttttt tagcttgttc
     1741 ctcttttcga gtccacatgc tcacatcatt accttttttt gcaagcccta aagctaatgc
     1801 acttccccag cttccagtac ctaatacaca tactttctcc atgtttaaca ccacctgagt
     1861 ataatatatt tactattctt tcttttgacc aatttttctc tctgtcccat ttaataatcg
     1921 ctttatattt gctctgtgat tatatattac tgatgcagtt aagaacagag ctactattaa
     1981 accagcttta ttttttacca taatcataaa tattggtgaa catcctattc ctacaacaga
     2041 acccaatgaa acatactttg ttatagctac caccaatata aatactgcta gacacattag
     2101 tgttattact ggatttacag ctagcattgc ccctagtgaa gttgcaacac ctttgcctcc
     2161 tctaaatcct aaaaccgctg gccaattatg acctgctaca acacatataa ctgccagata
     2221 tccagctaaa agagtatcta ttccaaccaa tcttgctgca aattctgata ttaaaacagc
     2281 cattacacct ttaagaacat cacctaaaaa tgtcattgct cctgctcttt ttccaagtgt
     2341 cctaagtaca tttgtcgaac ctgcattacc agaaccttga gtccttatat caaccccagc
     2401 aattcgcttt gcaactatgt atgaggtaga tatatttcct aaaagatatg caactacagc
     2461 aataattata taactaaata tctccactga atttcccccc tttagttata ttttaagata
     2521 tttatttttt atttttttgt ctatactcaa actttatgga agttccttca aatccaaaat
     2581 tttctcttat cttattctca aggtatcttt gatatgagaa gtgagttaaa tccttatcat
     2641 ttataaacaa tgtaatttta ggaggtctaa tgtcagtttg agtcccatag taaattttta
     2701 atcttctacc tttgtcagat ggtggttgat ttaacattac agcttctcca attacttcat
     2761 tcagtgctga agttgatatt ctcttagaat gttcatttga cacatattca actgtatcta
     2821 gtatcttgtt cattctttga ttagtttttg cagaaacaaa tacgattgga gcatacatca
     2881 taaatggaaa tttttctttt atatcttttg tgtaattact catagtctta ttatctttct
     2941 ctattaaatc ccatttattg attacaaata tgcatccttt tccttcatca tgtgctatac
     3001 ctgcaacctt tgtatcttgt tctgttacac cttctaaggc atctattact atcaatacta
     3061 catctgctct atctacagca ctcatagctc ttattacact gtatttttct attgtctcat
     3121 atattttact ttttcttcta agccctgctg tatctattag aagaaatttc tgtccatttt
     3181 tttcaaaata agtatcaatt gcgtctctag tagttcctgc aattggactt acaattactc
     3241 tttcttctcc aagtatctta ttgagtatag aactttttcc tgcatttggt ttacctgtta
     3301 tagctactct tattatatct tcttcatact ctgtatctaa tcctgctgga aaattttcaa
     3361 ctatctcatc taataaatct ccaaatccca tactattggc tcctgaaact gcaaaaggcg
     3421 ttccaaatcc caactcataa aaatcgtata tattatcaaa ttgactttga ctatcaatct
     3481 tattaaccac taagattact ggtttttttg tctttctaag caattgtgca atttctttat
     3541 cagcagctgt tattccagct ttaccatcaa ctataaaaag tattacatgt gacatgtcca
     3601 ttgcaagcat ggcttgattt ctcatttgag ataatattat atcttcgcta tcaggttcaa
     3661 ttcctcctgt atccactaat gtaaaatatt tatctaacca ttctacttca gcaaatattc
     3721 tatctcttgt aactccaggt gtattttcaa ctattgatat cctttttcct gcaaatttat
     3781 taaatattgt agacttacca acgtttggtc tcccaactac agctacaact ggcctactta
     3841 tactcatttt atttctcctt ccatcttttg ttttctattt taaaatttta tccacaaaat
     3901 cttttccttc attcaaacaa gggataattt ttattctcat aagttcttct aattgattta
     3961 attcaatatt atctaagaaa atttcttcat ctgcttttag catacttctt gttatataaa
     4021 gagcttctcc taattcttta tcttcaagtt gttcttttaa atccttagct gttaaaagac
     4081 ctgatacagt tattgtctct ccaaaaaact tgtttttgat ttcatataca tttacttgaa
     4141 gatttttaaa cttgtccatc atagcatctg ccatactctg tataaattca tatgcagaat
     4201 gaccagtagc tattgatact ttcttctctc tttttagaat cttctcaggc aatatattta
     4261 gatactcttc aatctctgtt tttaacttac gtatcatacc tacaccatct tcaaactgta
     4321 aaaaaccttc atattcttca tatccaggca attcactatt agatagtata taaaattcat
     4381 ctgataaaaa tgcaaatcga gtacctaatt tttcaagata tttctgttgt atttgatgta
     4441 tttgctctat agttttactt gcgctcttat cattaaatat atttaattct actagatttt
     4501 ctctatgctt tgtaatccca actggaacta tcgcaacact gtttacatac ggataaagtt
     4561 gagctagttc ttttattgtt ctatccaatt cctttccatc atttatacct ggacataaaa
     4621 caatttgaca gttcatttct atatgagtat ctgccaatct cttcattata ccatataatt
     4681 ttcctgcaaa tttattgctt atcatttttt gtcttaactc tggattagtt gtatgcacag
     4741 atatatttat aggacttatt ctatatttta taatattatt tatatcttct tcactcatat
     4801 ttgtaagtgt gacaaaattt ccttgtaaaa aagataatct cgaatcatca tctttaaaat
     4861 aaagagtttc tctcatacct tctggcaatt ggtctataaa acaaaacata cacttatttc
     4921 tacagctttt agcttggtca attatcggat ttgtaaattc aacacccaaa tcttcatcat
     4981 agtctttttc tatctcgtat atgtagactt cgccatcttt tttttgtatt tctacatcta
     5041 aatattcatc acttagtaag aatctatact ctattatatc atgtattggc tgttcattca
     5101 cagatataag taaatctcct acctctatac ctatttcttc tgcaatacta tctttataaa
     5161 ccttacttat aatattattt acttttttta catttacttc caatattttc accactcttt
     5221 tgtattcatc aatgcttatt ataatatatt ttaatatgca atttatttat ttattatatt
     5281 ttatgatagc atttttaagc atttcatgtc ataataacac attttaattc taatagtcaa
     5341 tataagtaaa tagctataaa atagattaga acaaataata ttcaaattta agtttcttaa
     5401 aaatttgaat attatttgtt cttaaaaaaa ttttataaat ttaatgtttt actatgatta
     5461 acgtcccatt tgtcatatca tgaaccattc cacctacaca ctttgacatc aacaatgcct
     5521 tattttgtaa gtctttctca tcatttgcat aaaatattgg acaacctcca ccatttttca
     5581 tattcttatc aagtgtaact atagctaggg tatattcatt taaacctata tccattccca
     5641 tcttatgcac ttcctttctt tcctatttta taatttttta gtgataagtt ttttccttta
     5701 gaacttgaaa gtattgggca tgactttacg gcttccatta ctttttccac attcttatct
     5761 actggtaaat aagcaatcat tatactttca tcctctttat tccttctagg taatggtacg
     5821 aatgaaggtt ctccataatc tccatataaa cctaaccttg aatatatatt gtatactatt
     5881 gcctgtcttt gacctgagtc aaataatatc cctgcattag cataatcttt atttttagga
     5941 attatttgta ttcctacacc tctattaata tatacttctc tatcactttt tagacctata
     6001 tttgttattc cctttaaatc tcctaccttc aatatagact catctacaaa actaatctca
     6061 gcaggcatta catcagctat gtctccaata ctttttctag taagaagttt ttttaatata
     6121 aaggctaaca ctaatccaca aattgaactt attaccaaac tcattccttc acttattttc
     6181 gtttctgcaa gtattacata atacaatcct attgttaaaa aagatgtaac tatacacata
     6241 taatttctta cttcataggt tctagctatc tcttctatga atgcagctcc tctctttaca
     6301 agttgaactt cctctaagtt ttgcaatgta tttcttctat ttgctctcac ttgtctaaat
     6361 tgctctgctg ctagggataa aaaagttata gatgtataag aacgatttat aagagctgga
     6421 accaataatg ctcctaatga agaagcgacg aatgctaata taagttgaga tagtaaaaca
     6481 tttggttgcg ttggatactg tttttggtct aaattcaaca ttataattct agatgttata
     6541 ccaataataa cagcaacaaa aaatactcta tccattaaat cactcctaaa atatttaatt
     6601 tgaaatttta catgtaaatc tacaaagtct ttctagaaaa atcactctgc tttccttttg
     6661 atgtttctat gataggtgta cttttcacta catctatcat gacatctata tctttgagta
     6721 ttggcatata tggtataacc acagtacttt tttctaaatt ggtcttagat atagcaacaa
     6781 tatcaacctc atctacatct ctatctatcc ccatatgaat aaatagatta tgtattatag
     6841 cttgtctttg ccctatatca tttactattc caaatgcacc taaatctctt ggtataactt
     6901 ctatggccag acattcattt ctatactttt ctcttgtact ttctagacct atgttagtta
     6961 ttattacacc atttaccatt aaaataggtc cttcaaaact tatttttgca ggaactatat
     7021 ctgctatatc tgctatactg ttccttctta aaaatcttct aaaaatcaaa ccaacaatag
     7081 ctcctgacac aatagctagg attgtgcaat aaaaaaagct aaggccatac tttcttgcaa
     7141 aaacgatata aactatcgaa gataccagtg cagaaaaaag acttatataa cttctagatt
     7201 catacgtaga tgctatttct tcaacgtaag cgtctccttt tggtactaat tcttcattat
     7261 cgatattctt tagtgttatt ctttcttgtt ctgcaagacc ttgaaactgt tgtattgcta
     7321 ctgcaaaaaa tgtcaaggct gcaaactcct tatctataag tgcaggtaat gctatagcac
     7381 ctaacgaagc tgaaagccca gatataatta tctgttctaa ataatcttgt ggtcttgatg
     7441 ggtattgttt gtctgtaact cttaaaacta atcctctaca taatattcct acaactaatg
     7501 caactataaa agagtgtcta aataaatttg cattcaataa actttcttcc attttctcag
     7561 ctccttctct tatatagttt gtgcaaaaaa caaaaaaaat tcccttctat ttaaggaaat
     7621 ttttttgtaa tattgtaact ataaattcgc taccaatgcc aagttcactt tttactttta
     7681 tacatccatt tagtgatttg actatatgct ttgttatagc caatcctaaa cccgttcctc
     7741 ctacatccct acttcttgct ttatctaccc tataaaatcg ttcaaatatg cgttctacat
     7801 cttcttttgg tatccctaca ccattgtcta taactttaat tacgattttt tcttcatctt
     7861 ttaacactac aacttgtaca atcccattat cttgggtata ctttatagca ttatcaacta
     7921 ggtttaagaa cacttgcttt atatagtctt tattggatag tatacttatt gatttatcat
     7981 cacatttata taaaacttct atattttttt cactagctga atgttgagtt atttcatata
     8041 cctctttaaa tacatcatat actacaacat tttccataag aagagtatct ttattttcta
     8101 caaatgataa cagcaatata tcatcaatca acctttttaa tctatctgat tcgctttcta
     8161 ttatttctaa gaatctattt ctagtattca catctatatt ttcatttgac tttagtgttt
     8221 caacaaaccc acttatagat gtaagtggtg ttttaagttc atgacttaca tttgcaacaa
     8281 aatcacttct catattttca agtttaactt ttttagtaat atcttctata tttactatcg
     8341 aacctattat tacgttgtta gtattctgaa gataaactgg gtctactttt atcttatata
     8401 cgatatcatc tttagtagta atattagcag tttcattatc tttagaacca ataaattgaa
     8461 gtatttgttt taatatttct tcttctttta ttacaacatt aatattatga ccttcaacta
     8521 attgatttgg ttcactcttt atcatttctt ttgcttcatc atttatcaga agtatatttc
     8581 catttacatc tatagctaaa attccatgag atatactctt taatatagac cttagctgta
     8641 aatgtttata ctgtacttct tctaaggttt tatccatatt ctttatcata tgattaaaat
     8701 ttctagctaa ttcgccaagt tctcctttgg ctgatatgtt aagcctagca tgatattcat
     8761 tattacttac tttttttgag acgtatataa actcttttaa atatttcctt aatgttattg
     8821 tgtatcttac agctaatata acaatggcta tggacaacat aacaataaaa aaatatatac
     8881 tatttaaatc ttccacaaga taacactcct aaaatataac ctttttaatc tattttgtat
     8941 cctacacctc ttatagtctc tatatatttt tccgatttat cttcatcttc tatttttttt
     9001 cttaaatatc ttatatgaac atctactgtt ctagtttctc catagtattc atacccccat
     9061 attttatcca aaagataatt tcttgaaagt acttttcctt tattttgaat taataatttt
     9121 aacaattcaa attccttaag agttaactct atcttttcag agcctttggt aacttcatgt
     9181 tttgataaat ctagttttaa attacctgta gtaagtatat tgttttcttc acctaagcta
     9241 gtcaaattat atctccttaa aacagaacta atcctagcta acaactcttt tatactaaat
     9301 ggcttagtta tatagtcatc agcacctatt tcaagccctt caaccttgtc attttccata
     9361 tttttagcag taagcattat aacaggaata ttttttaagt ccttatcact tcttattttt
     9421 ctcaaaactt ctattccact tatatttggt aacatccaat ctaaaagtat taaatctggt
     9481 ttaatttcct tagcctttat aaatccgtca aatccatcat atgaataact aacttcgtaa
     9541 tttgacacct ccaaattgaa ttttaatagc tcaactatat gcatttcatc gtctataaca
     9601 agcactttag tattcattaa aaagctcctc tctattttat ctgtagtacc gttcctattc
     9661 gttctgatgc cttattatgt tttctataag agtggaattt atctgctcta caactagtgc
     9721 ataaatttaa atttattata ttttcttttt taactccact acatctaagc atatactcat
     9781 taattttcca taaatctaaa tagtaacttc cctcttttat tatatagaac ttttcatcat
     9841 tatttgtaag aattgtgtta aatttttcta ctaaatcttc agacacttca taacaacatg
     9901 gtccaattga tggcccaatt atacatacta aatcttcagg aactgtttta tagcattcag
     9961 acattaattt tattgtttta tgacctatat tactaaaagt ccctctccaa cctgcatgag
    10021 ccagtcctat ggctttattt ttagcatcta taattgatat aggtacacag tctgctgtaa
    10081 acagtaaaag tggtactttt tccaagtttg ttataagtgc atctccatct cttcttttcc
    10141 ctatagtatc tttattgact atattaacta tatctgaatg tatttgagaa ttactagtta
    10201 gattttcaaa tgtcaaatca cattctttac atacttttaa catatcttct ctattttttg
    10261 catctatatt cttacatgtt ataactagat ttacagaatt taatttttca tcaagttctt
    10321 cacaattacc acttaaatta acatactctt taatatcttg tacatttttt atatttatat
    10381 aatctttcat catttgtctc ctttttctat cagtatactt ttaagttcat ttacaaaagt
    10441 atttatatct ttaaattgtc tatacacaga agcaaatctt acataggcaa cctcatctaa
    10501 ctctttcaat ttatccatta ccatctcacc gatttttttg gtttcaattt cttctataaa
    10561 acatttattt atttcatttt caatatgtac aacaatactt tcaatctcgt caatagaaac
    10621 aggtcttttt tcacatgctt ttagcaatcc atattttatc ttttctctat caaaatattc
    10681 tctactatta tcctttttaa tgaccattaa aggagttgtt tctattttct catacgtagt
    10741 aaacctcttt ttacatgatt cacactcacg tcttcttctg atagatttta aatctgtatg
    10801 tctagaatct attacctttg attctttata attacaataa ggacattgca tctatattcc
    10861 tcctttgttt aactatatct caaactcatc taagcttaat tctgaaccta tatttactat
    10921 tattgtatca caacctattt taagtatatc attccaaaat ataacttctt catcccctcc
    10981 tcttgaaaaa aaacttctac ttccatctcc aaaaatggta aatgagacta ccttaccttc
    11041 gtgaatatcc atgtctatgt ctattataaa ccccatcctt tttccatttt ttacatttat
    11101 aatttctttt tccattatat cagaaactct aatcatattg tactcaccct ttctttaaaa
    11161 ataaatatgt atcgaagtaa aaaaaaatga cctatatagg tcatttaaat taaaataatg
    11221 tttacagtcc atggattact tatacatatt ttctcatatt ctttaaagca tttttttcaa
    11281 ttcttgatac ttgtgcttga gatataccga tttcatcagc aacttctatc tgagttcttc
    11341 ctttgtaaaa ccttaaatct aataccaact tttctctact atttaatttc ttaatagctt
    11401 ctttaagtga tatttcttgc aaccaatttt catcagtatc ttttttatct tgaacctgat
    11461 ccataacgaa tattgcatct ccattgtctt gatatactgg gtcaaacaga gatattgggt
    11521 cttgtatggc atcaagtgcc attacaactg attctacttc taattctaat tcctttgcta
    11581 tttctgatac tgtaggttct ttagaatttg ttctaattaa tctttctcta acttgtagtg
    11641 ccttatatgc tatatctttt aatgacctac ttactctaat tgggttattg tctcttagat
    11701 accttctaat ttctcctata atcataggaa cagcataagt tgaaaacctt acattttggc
    11761 ttaaatcaaa gttatctatc gcttttataa gaccaataca acctatttga aataaatcgt
    11821 ctatattttc tcctctgttg ttaaactttt ttattacact caagactaat cttaaattcc
    11881 ctcttacaaa ctgttgtctg gcttcttcat caccattttt tatttgtaat aaaagttcct
    11941 tcatttgctt atttttaaga actggaagtt ctgatgtatt aacaccacat atttcaacct
    12001 tattaacttg catatatttt cagtcctctc tcaaaagtat tcatctattg aaattattta
    12061 catttccaat ttttttatac tttttaagaa gaattaattt agcattatac aaattttttc
    12121 atttcttttt gcaaccttga gattattttc ttttcaagtc tagatatgta ggactgtgat
    12181 attccaagca ttccagcaac ttctttttgt gttttttcaa tacctttatc aataagacca
    12241 aatcttaatt ccataatttg cttttctctg tcacttaatc tgtcaagtgc cataaccaac
    12301 aaatctctat ctatttcttc ttcaattatt ttgtatattt catcattttc tgtacccaat
    12361 acatcagata ataaaagttc gtttccatct aaatctatat ttaaaggttc atcaaatgat
    12421 acttctgttt tctttttatt attttttctt aagtacatta atatttcatt ttctatacat
    12481 ctcgaagcat aagttgccaa ttttatattt ttattcagtt taaaagtatt aactgctttt
    12541 atcaaaccta tagttcctat tgaaatcaaa tcttctacat ctatacctgt attttcaaat
    12601 tttctcgata tgtagactac taatctaaga tttctttcta ttaaaattga ctttacactt
    12661 tcatctgttt ctagcttttg taaaagctcc atttcttctt ctggtttcaa tggaggagga
    12721 agtatatttg cccctcccat atagtatatt ccctttggct ttattatttt taatttaata
    12781 cctaacatag taataaagct tattattctt tctttcaatc gtaacattcc ttgtccccct
    12841 taaattttca cctaaaatcc aggcaatata tttggattca ttattgctcc atattctcta
    12901 tcattaaaat ttgaaatacc aagtattata tttcctatct tttgctcatc tacttctata
    12961 tagtcagctt ttaagcctat tattatgctt gtcttattac ttccagcatg tttataaggt
    13021 attattctaa ctctacctga agttttatca tctaaacttc ctataatttc ttgtgctttc
    13081 ttcatatcta tatcttcata attaatattt aatagttttt ctggaataaa tccttctaac
    13141 acactagact taactattat tacatcactt tgacttattg ggtcttttaa caaattacca
    13201 ctatctacta aagccttaca gcaaaaactt ttttctagta agtttatatt tatagtcttt
    13261 gtaagctcct taatgtactt aagcattttc aaatctttat aaatgtattt taataattca
    13321 caactcacat aagtacatgc aattaagaaa gatattttta agtgacttat tcctgtaaaa
    13381 taaattataa aataagtgct tccagaaata aaaatgttta ctagataaaa aactaacgct
    13441 acatgaacat aatctttttt gctcttactt ctaaatgaaa ttaaagttat tatactcatg
    13501 attattattt taaatggtac agtaaatagt atattcattg atggatacaa ataagcaaca
    13561 gaataggcac ctcctaaact agcccccaat aacttatttt ttttaggatt atatctttta
    13621 gtaagaatag aagtacaact tataataata taatttatta ctaggttttc tataacatag
    13681 tactcaatat acaccatgta tcccccctta aaacctttaa acatattata agatagtatg
    13741 tacaaaattt tatgtcactt ttgatggtcg aatccaaaac aaattctatt taaaaagaac
    13801 aatgatatat gtcttatact ttatgtcgtc aaaagatatt tttttaatgc caaacaaata
    13861 gacttttttg tagaatccaa tatatctagg tagaagttat tcgctctact tttggtatat
    13921 ttacaaaaaa agactacata atttaataat atatcaagta aatcttttac ttatataaaa
    13981 tcaaattata tagtctgtaa attcaaatat aaattattgt gctagtaatc catttgcata
    14041 ttttgcagat tcatttagtg cttgttctgg agttatgtct ccagctatca tcatttgaag
    14101 ttgtttccat aaatattcat atatctctac tccatgaggt ccaactgtca atggtctata
    14161 catatgttta tctcttgtca ccatttcttc aaatcttggg tctccttggt atttttcacc
    14221 tttagcaact ggagctctag gtatttcttt gtggaaagct tccattgatt ctttgcttag
    14281 catatactca agaagtttaa atccaagttc tttattttta catgctgaca ttaatgttaa
    14341 atcatctact gaagcaaaac ttccaactgt cttatcttct aatgatgtta catatcccca
    14401 gtttaaatcc gggaatgatt tatcaaatgt acttgtagca gcagaggata gcattatagt
    14461 aaatgcagct tttcctggtc caaatacact ttcaatcatt tcattactat cttttgataa
    14521 agcatcttct ggaatatact ttttcatatc atttataaag tttgcagctt tcaagccttc
    14581 tgcattatta aatttaactg attttaaatc atcattatat atatctccac cagcttgcca
    14641 taaatacgga taccagttcc agtttaagct tccaaatact ttagaacccc aaccttgtgc
    14701 aagaccccac tggtcgattt ttccatctcc atcagtatct tttgttgctt tttcacagat
    14761 tcttttaaaa tcatcccatg tttttggagg tttttctcct aatttttcta gtatatcttt
    14821 attgtagtac aatacgcctg gattagcagc ttctataggt aaaccataaa taccacccat
    14881 catttttgca tcatctaagt atagataatt atctgaagtt cctgattttt ctaaataagg
    14941 tgttaaatct tctaccgcac ccatttctat aaattgagga aacatctctg catacatgta
    15001 tcctatatct ggaccttcac ctgcacttat tgctgttgca tatttttctg cgtagttatc
    15061 ccatggtact atctgaaatt caatttcaca gttattttct ttttcaaatt tttcaaatat
    15121 cggcttccat gcatctgcat catggtctgt taatggtgca gaccatacaa ctaatttttc
    15181 tttaccctct ttgcctttag aatcttcttt ttttgaacat cctgcaagaa ttgatactga
    15241 tagaatccct accataacta aagaaagaac cttttttagt ttaatcatgc ttaatcccct
    15301 ttcagacttt aatttgtctt tttcctcaaa tgtttacatt taattcagat ttaatattaa
    15361 caaataagat tactcttgtc taatcataaa aaactatact aactcctata actatatttt
    15421 ttttctatac ctattagatt taattcatac taaaaactta ctttttaaat tttatcaata
    15481 tttaaaaatt tttttaagtc tagccacaaa ctaactatct aaattgagca aaataaaaag
    15541 aagtctattg acttctttat aagcttacat attatcttct tcttcttagg aatgtaggta
    15601 tctccatgct ctcatcatca tcttcttcaa ttatacttgc ctttttagga ggctctattt
    15661 tttcctcagc aattacttct tcttcaattt cttttacagc ttgttttact gttgtattta
    15721 atgaagaacg tatgcttgat ttaggtttag tatctaaatc taaggcacca ttttgtaatc
    15781 cttcaaatcc tgtcgcgatt actgtaatca ttatttcatc acctaaatct tcttttatag
    15841 aagctccaaa tataacattt gcttctgggt cacaggattc cataactaaa gttgatgcct
    15901 cattaacttc aaataatcct aagttaggac cacctgtaac attaagaagt actcctttag
    15961 ctccttgtat tgatgtttct agaagtggag attgaatagc ttgtcttgca gcatcaattg
    16021 ctctagtttc accactagca ctacctatac ccatatgagc aagtcccttg tctttcatta
    16081 tagtagtaac atctgcaaag tccaagttta ttaacccttc aactgctata aggtctgaaa
    16141 ttgattgtat accttgtttt aacacatcat ctgcaactgc aaaagcatct aacattgatg
    16201 tatttttttg tactatctgt aaaagtctat catttggtat cgttataaga gtatctactt
    16261 ttgatttaag ttctgctatt ccaccttcag cgttcttcat tcttatctta ccttcaaatg
    16321 caaaaggttt ggtaactaca cctactgtaa gtatccccat ttcttttgcc aagcctgcaa
    16381 ccactggagc agctcctgta ccagtaccac cgcccattcc ggctgttaca aagaccatat
    16441 cagctccttg aagtaacttt actatttcat ctttactctc ctcagcagct cttttcccta
    16501 cttctggatt tgctcctgct ccaagtcctc tagttaattt ttctcctatt tgaactttat
    16561 attcagcttt tgatgtatat aaagcttgtt tatctgtatt tactgatata aattcaacgc
    16621 cttttagttg agcttctacc attctattga cagcattgtt tccaccgcca cctacaccta
    16681 ttactttaat ttgtgcacat tcttctaatt ctacgtcaaa gtttagcatc ataaaccccc
    16741 ttctctcatg attcaaagtt atcgtatgta ttaattatat cattttcttc ttttattttg
    16801 ttaaccttta agtaacatat tccacaaaaa cgtaaatttt cctttttaaa tttcttactt
    16861 atattgtcaa atattactct ttaagtataa aataatttac gtatatatta aatgctcttt
    16921 aatttttacg ttttttgttc ataatattac taataatata tcgtctaatt atagataagt
    16981 tattaaacaa tctcatacca aaaactaata cagcaacata gtaaagagaa actcctaact
    17041 tttctcctat ataagtcaat aatatagcca atatagcatt acctatgaaa ccagtaataa
    17101 aaattacatt atcatagtta tcttcaaaat tggattttat cgctccaaaa atagaatcca
    17161 ttattgcaag aatagctact gacatatata tggaataatc catagaatat gtaaatggaa
    17221 tatagtaacc tactactaca ccaaatgcta atcctcctaa aacccataac ataaatcttc
    17281 acctctttat tttactttga aacacaacta cattacaatc ctttttatta atttttttta
    17341 ctttttcttt atttttttgt atttttcgtt ttattttgca aataaattca ctttatttcc
    17401 attattttcc tgaactttta tattaattcc atatatatcc ttaagagatt tagcatatga
    17461 ttctggagag tttatagctg cactcaataa atctatatct cctatagctt ttattataaa
    17521 tggttgtcca tatgtagtat catttactgt aatagtagct cctgaacatt ttatcttact
    17581 tgttgctagt actctttctc catttataga aattgctctt gctccagcct tttttaaatc
    17641 atttaaaacc ctcaaaatat ctatatcatg tataatcaaa tcgtttggat tctgtccgtc
    17701 ttttaactct ctctcactat ccctcatagt aatagttatg cctggccctg taacagttga
    17761 ataaccactt aattctttaa cttcttcaag ctctttttgc ataagtgttt ggatagattc
    17821 atttccattc ttttttgctt ctttatattc attcaaagtt tttttgttct ttttaagagt
    17881 ttcttttaag ttttttattt ctttttttgt attttctacc tcactttcaa tactttcagt
    17941 ttgacctaat gttatgtata ctctatttgg gtctagtgat tttatataag ttgatatgaa
    18001 aacgcctaat atgaatgctc ctaatataac tattttcttt ttcatactat tatttcccct
    18061 ctgctttgtt tatatcacta tgaccattta tttctatatt gtcttctttg gtcactttta
    18121 ctttggcatt ataatatgtt tctatttccc agacaatacc atactttata ttaagtgccg
    18181 aagccaatgt atcagaatca ccaattgctt ttattataat tggttctttt atgacagtgt
    18241 cattcatata aagcttgtct tcttttaaat acatatatgt attagaaact attctctggt
    18301 cgtttataga tatggcactt gcttgtgctg aatttaattt atttatcatt gataataata
    18361 aatcataatt atatagtata ctatcatttt tatcagagtt tcctaagtct tttgtagaat
    18421 ttatctcaat ttttatgcct gttccttcaa catctgtata tccagctaat gtttcatatt
    18481 tttctatttc tgacttaagt atgtcttgtt ctccttcatt gtttttgcct ctataaatct
    18541 ctatatcttt cttcacacta ttaacttctg attgtagatt ctctttttct ttttttaaag
    18601 atttaagttc ttctgtaagt tgctctcctc ttttaaaagt tgtcatccct ctattttcta
    18661 tattaataat tttaagttgt aaactaacaa gaacacccaa tacaatactt cctatgatta
    18721 tacttttatt tataaatctt tttttcaata tatcacttcc ttacaattgt ctatccttct
    18781 ggactataca ctgccaactt tccatagtta agatttatag tccctccatt agtattttta
    18841 ttttgcaggt caatcagaat tttacttacc ctagatatat tataatctaa gttatcatca
    18901 ttagaaagta agatttttat attacttctt gtaagcattt ctatattgct ttcttttttt
    18961 aagtttacat aatttatttt tctataaatt ccttcttctt ttaaataatt taaagtttta
    19021 aatacattct ccttagtttt ataattttta aattttatag atttattatc atcaatgcta
    19081 taatctacat ctactatcaa gtctttttta ctttcattag aacctcttag ttcttccaat
    19141 aaatttcctt ttttatctat ataacaataa ttatcttcat cttttaatac tgcaaatatt
    19201 tctttttctt ttaaacttat aattatctta ttgggaagtt ttctttttat ttccacattt
    19261 tctatatatg gattt
//
LOCUS       DACULQ010000065        19271 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.065, whole genome shotgun sequence.
ACCESSION   DACULQ010000065 DACULQ010000000
VERSION     DACULQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.065"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            326..508
                     /locus_tag="JA154_09170"
     CDS             326..508
                     /locus_tag="JA154_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996036.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            complement(796..1019)
                     /locus_tag="JA154_09175"
                     /pseudo
     CDS             complement(796..1019)
                     /locus_tag="JA154_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431039.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
     gene            1340..2254
                     /locus_tag="JA154_09180"
     CDS             1340..2254
                     /locus_tag="JA154_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996037.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRYYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2644..3291
                     /locus_tag="JA154_09185"
     CDS             2644..3291
                     /locus_tag="JA154_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HAT7996038.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3458..4027)
                     /locus_tag="JA154_09190"
     CDS             complement(3458..4027)
                     /locus_tag="JA154_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAT7996039.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(4032..4931)
                     /locus_tag="JA154_09195"
     CDS             complement(4032..4931)
                     /locus_tag="JA154_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAT7996040.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGILLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4942..5091)
                     /locus_tag="JA154_09200"
     CDS             complement(4942..5091)
                     /locus_tag="JA154_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996041.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(5084..5668)
                     /locus_tag="JA154_09205"
     CDS             complement(5084..5668)
                     /locus_tag="JA154_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAT7996042.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            6057..7382
                     /gene="cdeA"
                     /locus_tag="JA154_09210"
     CDS             6057..7382
                     /gene="cdeA"
                     /locus_tag="JA154_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HAT7996043.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFISSLLVFIIALVFA
                     PFIVRVFVNPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(7477..8271)
                     /locus_tag="JA154_09215"
     CDS             complement(7477..8271)
                     /locus_tag="JA154_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996044.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(8272..9147)
                     /locus_tag="JA154_09220"
     CDS             complement(8272..9147)
                     /locus_tag="JA154_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996045.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(9151..9519)
                     /locus_tag="JA154_09225"
     CDS             complement(9151..9519)
                     /locus_tag="JA154_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7996046.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(9702..10361)
                     /locus_tag="JA154_09230"
     CDS             complement(9702..10361)
                     /locus_tag="JA154_09230"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088002.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HAT7996047.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(10397..11584)
                     /locus_tag="JA154_09235"
     CDS             complement(10397..11584)
                     /locus_tag="JA154_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAT7996048.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(11661..12779)
                     /locus_tag="JA154_09240"
     CDS             complement(11661..12779)
                     /locus_tag="JA154_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT7996049.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTDVDIEVFENTNNQIGLDLGIKEFCISSCGEFIENPKYFKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLTTKLIKENDIICIEDLQVKNM
                     IKNHKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKN
                     LNIREWICPSCNETHDRDINASRNILKEGLRLITIQNK"
     gene            13054..13203
                     /locus_tag="JA154_09245"
                     /pseudo
     CDS             13054..13203
                     /locus_tag="JA154_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589351.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13430..13834)
                     /locus_tag="JA154_09250"
                     /pseudo
     CDS             complement(13430..13834)
                     /locus_tag="JA154_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14308..15198)
                     /locus_tag="JA154_09255"
     CDS             complement(14308..15198)
                     /locus_tag="JA154_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HAT7996050.1"
                     /translation="MIMDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEE
                     VELAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKY
                     DYKKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLRE
                     PTIDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIH
                     EVEQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIE
                     AKAIRKLRHPSKLKQLKHFY"
     gene            complement(15483..15959)
                     /locus_tag="JA154_09260"
     CDS             complement(15483..15959)
                     /locus_tag="JA154_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996051.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            16225..17400
                     /locus_tag="JA154_09265"
     CDS             16225..17400
                     /locus_tag="JA154_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HAT7996052.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(17481..18284)
                     /locus_tag="JA154_09270"
     CDS             complement(17481..18284)
                     /locus_tag="JA154_09270"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087996.1"
                     /note="catalyzes the formation of L-proline from
                     pyrroline-5-carboxylate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAT7996053.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(18324..18908)
                     /locus_tag="JA154_09275"
     CDS             complement(18324..18908)
                     /locus_tag="JA154_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT7996054.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         6579 a         3056 c         2153 g         7483 t
ORIGIN      
        1 aataatagat tacaataact aaaattttaa aattaaattc cttaaaagta tgattaatta
       61 taattatata agtatattct tattacactt atattaattt atattaaata tctactaact
      121 caaatattca taattaaact ttaaactgga taaattttct tttaagaatg aaagtttatc
      181 cagttttgcg attataataa ccagttttac atatccactt gtaaaatata tctaagcaat
      241 atatcatact atatagattt atgagtgaaa taatatctgc tatttactga atatttttat
      301 ttttcgtttt ttagggggat tagttgtgtt taaagataag attgatgaat gtgttcatat
      361 aatgactgca tatattgcta gtttaaaaga atactattca ttcatagaaa ctcaaattgg
      421 tgattttatt aaaaaatatg gagaagatgt tgtagagtta tgtctccata gagtgatgat
      481 tctactatgc gaatgtggtt tggcataaaa ttaaatttat taaaatgggt aagaagaatc
      541 ctatataata ttcttcttac ccattttaat atatcaattt catagcttat cgtttaaaat
      601 attttacatc cattactaga caactttaca ttctcttttt aattctagta tatttaaatc
      661 attttagttg ctccttattt attcttatta cttgttttat tttataaata aaataattct
      721 ttttaataat gttttttgtt gatattccta tcacacatcc aattatttat cattgtcttg
      781 aaattgtttg catacttaat ttaatccaat tgaaacaccc cagcatagaa tcccctgtaa
      841 aatagaagtt ggattaaatc ctaacatcta tacagaaaat actactccaa gcacaagcaa
      901 tataatggaa tatatttatc acttaatttt ttatactttt taaatcccat acctaaagca
      961 ttaagtgcag ctattagaat taataattgt tgtggtataa aattatgctt gcttcatatt
     1021 tattcatttt ttactataaa agttcttgtt ttacaaaaaa catttgtttt gtatcaaaat
     1081 aatacttgta aattacataa ttctcataaa aatttctagt attacttgta ctcttaataa
     1141 ataatagcca acaagtacct taaatcatta tatctatatc taataagaaa cacagtaaga
     1201 taattttttc gcccataact taataaaaac tgttgatatt agtagcaatt gaatttagtt
     1261 aatttattta taatatacat aaatttaaat attacgaata aagtattata aataggatac
     1321 tttctaaagg gaggaaatta tgatagataa tcaaaaatat gttattttat cactagaatt
     1381 acatttattt ttttcaagaa ttatgaaaga gcatgctctt tttttagaag caggattcac
     1441 aaataaaaat tataatcttg ctatggaagc tgaccactat aaaaagcaat ttgaagattt
     1501 attatcatac actgttagtg ctagtaatgg tataattaga cctgatatat tatattcaga
     1561 agagcttgta actactctca catcagttgc agaacaaaaa acagaagagt ttacagggat
     1621 agaaataaac aaaaacatca ctacaagaga attaaatcta caaagtggtg taaacccaca
     1681 agttggtcaa gacttagtga actatgtagc tcaacttaac tctgatgcaa taagattact
     1741 tgatgggctt attaatttta aagaaagagt cttagatggt gtactatcat gtactatatt
     1801 tacatcaaac taccctctac ttcttgaaca tataatacat gaagcaaatt tatatcgtta
     1861 ttatgtagtt gaccttgaaa ataaaataga tattgagtca aaaaacgcta aagaaataga
     1921 attattctgg gaccatatta tgatggaaca tgctctgttt atgagaggat tactagaccc
     1981 ctcagaaggt gaactaataa atacttcaaa tgattttgct ataaaattta atgaattaat
     2041 tgaaaaaaca aacgaaatga ctgattctaa tatcaagaac attacagaag aaactctaaa
     2101 tgaaactgtt gagtttaaag attttaaaga agcaggagca tcaggaatag aacagtgtaa
     2161 gataaaatct ataatattac cacttttagc agaccatgtt ttaagagagg caaatcatta
     2221 tattagaata ttggagagtt ataaaaacat gtaattaaaa attactttta aaataatctt
     2281 tgcatattta gataaaataa aggtacagtt aactgtacct ttatttttta taaacttata
     2341 aaatttaaac aagttattca taaaataaaa taataatcat ctataaccca caactttatt
     2401 ttatattaaa taaaaagtat tagtgattat gttttatagg tatttcttta ttcagttatt
     2461 tattaaaaac taatagtaaa tcttatacta atcgcatatt tatgttataa ctatattgta
     2521 actattttcg taatcttagg aaaagattac ttataaaatt aaccttatac aataatatat
     2581 cgtataatct taattagcat aatagttaca atattaaaat ttaaatatgg gagtgatttt
     2641 agtatgataa gacaaacacc attagagaaa cgatatgaca agctaggtcc tcatattgta
     2701 acagcactca aaaaacgtta ttttgatgct tattactgta aaactagaaa tgaagctttg
     2761 caacaaattt tagatttaat tcctcaaaat catgtagtat catggggagg atctgaaaca
     2821 cttaaagaaa tgggcgtaca aactgctgtt agagagaaag gatttaaagt tattgataga
     2881 gatttagcaa aaactccaga agaaagaact gaaattatgc gtcaagcact tctatgcgat
     2941 acttttttga tgagtagcaa tgcaattagt gaagatggac aactctttaa tattgatgga
     3001 aatggcaatc gtgtagctgc aatgatattt ggtccaaaaa atgttatagt tgtagctggt
     3061 atgaataaaa ttgtaaaaaa cattgatgat gcaatacaaa gaactagaac aattgcatct
     3121 ccagcagttg ttcagcgttt tcaagatgtc aatacaccat gctatattaa tggaagttgt
     3181 atagattgca caagccctga aagcatttgt gcatacatgg ttactacaag aatcagtcgt
     3241 ccagcaaaaa aaataaaggt aatattggta ggagaaaatc ttggtctata aaatatacat
     3301 acatgtaaat agctctataa ataataaaat catatgaaat aacttatttc taaaagtggt
     3361 agaataaaag cttctacaag actttcattc taccactttt atattctttt aatttgtgtt
     3421 tctatattat taattgctcc gattttcata tatattacta actattttta ttttcataat
     3481 ttttttgttc ttcatcaagc tgtttataat aatctttatt atagtcagct agataatcta
     3541 taccacctcc tgtaatcaca tttaataatt tcattgcaga ttcttgatta actttacaaa
     3601 cataattatc accagattta acaagctcta atgtaatatt aaattctttt ttctttgcag
     3661 aatcaatatc tttcatagtt ggatttggca tcatttctcc tagtttagga ggattctttt
     3721 ttaaaataga aaccatatca gtattgttaa tttttaaatc tacagtcact ttatccttat
     3781 tattctttat atctgaaatt tcccaagtca tatttttaga atacatttta aatccttctt
     3841 tatcttcgta tataagatta cctaaccctg attttgggaa atacttatca acagtatctg
     3901 catcttgtgt ttttattgaa ttaaacatac tatccacttc attttttatc ttattatcat
     3961 tagtacaacc agctaacacc agtactgata ttgcaaacat tataatacat ataataaatt
     4021 tcttcattaa attatatccc cctatacttt tttgcacaaa tattattctc ttttttatct
     4081 tttgaaaaag ttgtagtaat tgcattttgt ggacaaactt ttatacattc accacaccta
     4141 atacagtctg gactatttac atctttatat attggtatat caagcttaca ttttgtttga
     4201 catgctgtac aatttgtaca tttatcttta tcaattttaa atctatatat tgaaatagga
     4261 ttaaaaaaac catagattgc tcctaaaggg catatatact tgcaaaatgg cctataagta
     4321 atgattgaca tcaataatat caatagcaaa atacttaact tccagctaaa taaaaatcct
     4381 attgattctc ttaaattctc atttcctaaa ataagtagta ttcctcccaa aagtgttcct
     4441 gaagggcaaa tccatttaca aaaccaagga tttccacccc ctgctacatt tacaatagca
     4501 actggcaaaa taattacaaa cattattaag actatatact ttagatattt tagatattta
     4561 tcaccataaa ctttttttat ctttactgga aagggtactt tatgtagtaa atcttgtaca
     4621 agtccaaagg gacaaagcca accacaaaca aatctgccaa aaaaagcacc aaatgccatt
     4681 aaaaaaccaa caacataaaa tgagaattta tactctctgc ttgataaaac tgcttgtaat
     4741 gaacctattg gacatgctcc aagtgcacct ggacatgaat aacaattcaa tccaggtaca
     4801 cataattttt tactttctcc tccatatata gttccttcta aaaatccatt tacatatcca
     4861 tttgttaatg ctgtaaatgc aacttgagta ataagtctta tattattatt taccttagat
     4921 aataacttca tccctcttct cctaaccaat accaatacac tcaagacaaa tcataattgc
     4981 tttagttaat actgtatttg tttctccacg atatgtacct aatgcaataa atataatact
     5041 aattgcaata atagaaatgc cttttttatt atctttaata aagttactca ttggctttta
     5101 ccatttttag tctttcatct ataatttttt tccaatcttc cttactattt gcacctacaa
     5161 ccttctcacc cacaaagttt cctttcttgt ctacaaaata agttgttgga aactccataa
     5221 ctcttttaaa atcagttttt ttaagttctt cagatacagt caattgttgg taagttgcat
     5281 ctgacttttt cataatgtct ttaactaaat ttttctcttc atttgaaaga ccagttctca
     5341 tatcagtttt atcaacttct acaactaatc cctttatagc tacattggaa ttattactct
     5401 catattcttt tgataatgct tcaagctctg gcatttcacc cacacatggt ccacaccaag
     5461 tagtccagac attaataaga gtaagttctt tatttgaaaa tatctctttt gttaccttat
     5521 ttccatcaac atcctccgta tctaaattac taaaccattc actattattc ttgttttttt
     5581 ctactggctt agattctttt ttgctacatc cagaaatcat acctaaaaca caaatacatc
     5641 ctaaaactac tatgaaaaac cttttcatat ctcacactcc tagaatttat atagtatata
     5701 aaatcgctgc ttgaaataaa aataacttat cttctatatt aaagtaatta tatttaataa
     5761 ataagagcta atttaaaaaa agatatacag ttaaaatcta tttcttttca gacaaaatct
     5821 tatatatata taatagcttg taaatcttaa gattatatac atatatttct aaagattttc
     5881 ttaagaaaca gttagatttt actaatataa gtttattaat tagcattata ctaatttatc
     5941 ttaatatgat tttttgttga ttttaagctg tttatcgtat aaataaaatt tttcttgact
     6001 atatagttac tatatagctt attatatata gagagtaata aaaggaggta aaatttatgg
     6061 aaaatttatt tacaagaaaa ttcactactt ttgaatttct aaaatttgtt tctcctgcaa
     6121 ttatatccat gatatttata tctttgtata caataataga tggcatcttt gtatcgacat
     6181 tagttggttc tgatgctctt gctagtataa atattgtact acctataatt aaccttgttt
     6241 gtggatttgg aataatgatg gcaactggtg gaggtgctat cgtttctata cgtatgggtg
     6301 aaaatagaca ggatgaagcc aactctacat tttcttttat agttttgttt tcattgattg
     6361 ttgggatttt attcacagta atctcatatt tctttatcaa agaaatatct attttgcttg
     6421 gtgcaacaga taagttatta ccatattgta taacttatgg taaagttatg attttatgta
     6481 caccatttta tattttaaaa tttatatttg agtactttgc aagaactgat ggaaattcta
     6541 aatttagttt atttctatca gtcattggtg gtgtaacaaa tataatattg gattatgtat
     6601 ttattaaata ttttggaatg ggtcttttag gagctgcagt tgcaactgct ataggtatta
     6661 ttttaacttg tgttttaggt attatttact tcttatctaa taaatctaca ctaaaattaa
     6721 gaaaaccaaa aaccgatttt agacttataa gagatactat gataaacggt tcttctgaaa
     6781 tggttacaga attatctaca ggaattacaa cattcttatt taatgtagta gctttaaaat
     6841 tagcaggaga aaatggactt gctgctctta ctatagtatt gtatgctcat tttttaatga
     6901 catcagtcta tctaggattc gctgctggag tgtctccatt aataagctat aattttggtg
     6961 ctgaaaacag tgataaatta aaagaaacat ttaaacattc tctaaaattt atatttattt
     7021 cttctctttt agtgtttatt attgctttag tatttgcacc atttattgtt agggtctttg
     7081 taaatccaga taacacagta tttaaactag ccttacaagg attaaaaata tttgcatttg
     7141 cttttttgtt tgttggtata aatatatttg catcaggatt ttttacagca tttcacaatg
     7201 gaaaaatttc agctattata tcttttagtc gtgcctttgt ttttataatc ataggaatca
     7261 taattcttcc tcctatgtta aacatgactg gattatggct tacagttcca tttgctgagg
     7321 ttataaccat atttatatct attctattta taaaaaaata taaatgtaga tataagtatt
     7381 aaaaattaat acttaaaatc aacatttatt aaaactaata aacctgcttt aaaatacaaa
     7441 tttacaaatt tgatttttaa gcaggtttta ttttagttat aataagtcat ttgcatactc
     7501 tttaattggt gcttttctaa gtaataaaat tataaatatt atgcaaataa ctataattcc
     7561 tacctttgtt ccaaataaaa taaaaggatt ctgaaatgaa taaataattt tttctaaata
     7621 caatagcatt ttatgcatat ttttagttgc aaaattaact atataaatta ccattaatat
     7681 cggtaatcct atcaatatag taatactagt aacttttcta actctatatg taaaagctcc
     7741 aagtaacact cctataaaac ctatacatat ttcatttata catatttgta caaaataatt
     7801 taaataagtt agtggcatac ttgatgttga aggatctatc agtgctgata tagatgtcca
     7861 aatcatatct ctctttagta aaactgatag ttctatactg taacctgtta taatttctat
     7921 aaataacttc gataaaaaaa tcaaaactat acctaaaata gaaatcacaa agcttaaaat
     7981 aattaaatct atgacaatag cttttataca actttttcta tctgctctta tacttaaagc
     8041 tcctgaaaag tctttatttg ctatatttac tccatacaca aatagcagta tagttatata
     8101 tgttaaaata ggattcacaa tcgtggataa attagtttta gatgcttctc caaaaatctt
     8161 gagaataaac actggcaaaa tattcataac aattcccaca atcataaata taattatata
     8221 caatttagta tttttattaa aaaattttat atatggcttt aattcattca attacaattc
     8281 ctccttctta gtcatgttaa caaacatatc ttgtagccct atgtatccta catcaatatt
     8341 tgaattttta agtatttttt catcattttc actaaaatca ccatagtaaa aataagcaac
     8401 tgcatttcca aatgcctttt taggttttat attttttata cattctaatt tttctaactc
     8461 ttctttattt ccagtaatat aatgagcttt ttgttttatg acatcaattt cttcatctat
     8521 aataattttt ccctcattta atattattac atgctccagt aaatcatcta tttcatcaat
     8581 caaatgagtt gatattatta ttgtccttgg attttttata taactatcta aaattatgtt
     8641 ataaaactct tgtctattta ctgcatccaa accaattgtt ggctcatcaa atatggtgat
     8701 agctgaatta gaacaaatac ctattatatt tgaaagtata gttttcatac ctcttgacag
     8761 ttgtttatac ttttttttag tatttaaatc aaaatatttg cataattctt tttctaagcc
     8821 tttatcataa cttttataaa aactagaaga aaattcaaat atctgaccta ctttaaaatc
     8881 tggactataa aattcttttt ccctgacaat acaaacttct tccaaaactt ttatatcttc
     8941 ttttgggttc tttcctaata tttctatctc accttcattt tgaattaact gatttaccat
     9001 tatatttaat aatgttgttt ttccagctcc attcttacct aataaaccat atatcttatt
     9061 tttttcaaag tttagagaca tattattaaa aatattttgc ttcttaaaac tgtgagataa
     9121 attttctatc ttaatagaac tactcatata ttaatcctcc ttgaaattaa ttataatatc
     9181 aaccaattct tctctattta tttctaattt gttagcttct tgaagtaggt tttttacaaa
     9241 attatgttta aaattttctt ttctagcttc ttttataata gtttttgcac catctgatac
     9301 aaacatccct atccccctct tcttatataa aatattttta tcaaccaata tatttatacc
     9361 ttttaatact gtagctggat taattttata aagctttgaa agctctgttg ttgatggaac
     9421 ctgttcttct tcttttattc catttgatag tatctcatct tcaattgctc gtgctatctg
     9481 aataaatata ggttcttcat tatttaatac taatttcata ggttcctcct ttctccatta
     9541 gttagttact caactaatta actatataac ttataatatc ttgaaaaata ttttctgtca
     9601 atactaaata aaaaacagag ggaaaattcc ctctgttttt tgttctttat atttataatt
     9661 aaattttgat ttttgtaatc ctatcccata agttaaaaac tttaataatc tgttgtatct
     9721 tcatttttta atattttaca tattgagcta gttcctaacc tttctgctcc tgcctctata
     9781 aatttttctg catcagaaat tgatttaact ccccctgcag cttttatttt tacatttttt
     9841 ccaacatgct ctttcataag ctttatatct tctaaagtag ctccacctgt tcccattcca
     9901 gtagatgttt ttataaaatc tgaacctgac attgttacta tttcacacat ttttattttt
     9961 tcatcttcat ctaaataaca tgtctctata atgactttta atactttatc tcctatagct
    10021 ttttttattt cttttatttc ttttcctata ttatcataat ctttattttt tatatctgaa
    10081 atgtttacaa ccatatcaac ttcatctgct ccattttcaa tagcatcttt tgcttcaaat
    10141 acttttgtag cagtagtttg atatcccaaa gggaacccta tcactgtaca tatctttatt
    10201 ttacctttta gatattctga tgctcttttc acatatgatg gtggtatgca aactgatgct
    10261 acactcatat ctacagcttc atcacataaa attttaatat cttcccatgt agttgtagct
    10321 tttaaaatag tatggtctac tgtttttaat atatgtttca taaaatcctc ctaaatttat
    10381 aattaatata attagattac ttttttaaat gcaattttct ctttgcaata ttcagtatca
    10441 tatcaacctc ttcaacttta aaaatagtca ttagtagtgt ataaattcca cctccaacta
    10501 taactgaaat agctagtgag acaaactcat taaatgttcc tactcccaaa attccaaata
    10561 taaatttata cgtaaagtat gataaaacac ccataattaa agatgcaact accgatttca
    10621 aagttgcttt tattatttta tcttgtccaa aatatccaac ttttctcttt aggttcaaga
    10681 agagtaacaa tatacatgca attgatgatg aactagttgc aagagcaaga ccaccatgag
    10741 ccattggttt tattaaaact aagtccaata ctatatttac ccctacagat ataatcccat
    10801 ttaccatagg tgtttttgtg tcttgtaatg aataaaaaac ctttcccaaa atatctctta
    10861 agccaaatgc agtcatacca actgcataaa aaattaaggc agttgctgtc atttgggttg
    10921 ccctcgcatc aaaagcacct ctctcaaaaa tgattctaac aacaggagtt gcaaaaaaaa
    10981 tagaagcaac tgaaatagga atcatagaaa ttattatcat atttatacta gatttaactg
    11041 aactagtaaa cttcttttga ttattttctg cacttaactt agataacata ggatacataa
    11101 cagatactat tgaagctgta aatgtccctg taacaaaacc atttaatcta tctgcataag
    11161 taagtgctgg aatgcttcct ttaactaatg ttgaggctaa agttgtatct accaatgagt
    11221 tcacttgatt aacagcaaca cctataaata ctggtgaaag taatgctagt aatttaataa
    11281 ggctatcatc tttaaagttt aagtagtata agtatttgta gttcgttttt ttaacaaaaa
    11341 acatgtaaaa gagcagttga actaccatag caaccacagc accaattgga agtatgtatg
    11401 gtccaaatat ggtacttaac attattgaaa taattattac tatattatag ggtatacttc
    11461 caaatccaac tacaataaag ttctctttaa tttgtaaaaa tgctgacata acacttgtaa
    11521 tacctatgaa tattattcct gttctgaacc tcccatgact aaagtcacag ggttcctgct
    11581 tcatagaatt ttgcatacta aaatatgtct tactaattct ccacaggcta tccccgtagt
    11641 tcctacggtt cttacatacg ttatttattt tgaattgtta ttagtcttaa tccttctttt
    11701 aatatatttc tacttgcatt tatatctcta tcgtgagttt cattacaact aggacaaatc
    11761 cattctctta tatttaagtt cttaacttcc tcgtttttat acccacattt attgcatatt
    11821 tgtgaacttg caaagaattt acctaccttt acaatttgtc taccatgcca attagccttg
    11881 tattctaatt gacgaataaa ctctgaccat gatacatcag aaattaagcg agatagttta
    11941 tgatttttaa tcatattctt tacttgtaaa tcttctatgc aaataatatc attctctttt
    12001 attaatttag tagttaattt ttgtagaaaa tcatttcttt gattagctat atgttcttga
    12061 agtcttgcaa cttttaatct tgctttattt ctatttaaac taccgattgt ttttctcgat
    12121 aattctcttt gtaatttagc aagtttgttt aatgacttct taaaatactt aggattttct
    12181 ataaattctc cacaacttga gatacaaaat tctttaattc ctaaatctaa acctatctga
    12241 ttgttagtat tttcaaatac ttcaatatct acatcagtac agcataatga tacataatat
    12301 ctaccacttg gttctttaga tatagtagca ttaagtattc taccttgagg tacttgttta
    12361 tcccttatct taaccatacc aagtttaggt agttttatat gttgaccaca atacataatg
    12421 tttccatttg taaagttagt tttatatgaa aatctattaa ttttctttga tttaaattta
    12481 ggaaatcctg ctttttcttt aaagaatttt ttatatgcat tatctaaatc ttttaaggcg
    12541 ttttgtagtg aaaatttgtc aggttcttta agccatttta attctttttt taaattggtc
    12601 aaatcagaac tacactgttt ataagtaaaa gtttctttat cgtttttata aacatctatt
    12661 cttttagcaa gatacttatt atatacaaat ctacaacatc caaaagtttt gttaattaac
    12721 tcttgttgtt ttttatttgg atacattcta aacttataag cttttttcac tgctatcacc
    12781 tcactttaat tgttttttga tacacttttt aatgatttta tcacatttta tcacatttaa
    12841 tatattatat aatgctgtgg taaacaattt attaatcacc tataataatg ttaacaaatg
    12901 taattattta aaacaattat ttagaaggtt ttatatttaa ttttaaataa catatttaag
    12961 aaccgtaagt cttaactttt atcattttga ggttgtcgtt cacataagtt cgctactacc
    13021 tatgcagttc tcttatgaac ttcttacatt ttcatgtaag cacagactat atcttatcct
    13081 tcagcattat ccgttaaggt ctacccactt ccactaccaa tagcttgtag tgtacttccc
    13141 tcaagaggaa tagtcgttga agtttctcct gttcggagtt tacctgctga ttgcccattt
    13201 taaagtactt aggatttaac cttataccat ctaaccaatt ttttctactt tcgtaacttt
    13261 cacacttagg tttatttcat ccttatgttt tagtttgatt agctttaggg gtttccagca
    13321 attcaagtag tattggatag gttctatcct atctctacat ataggtttcc ctatatgctg
    13381 actattttag ctactaactc atgactaaag tcacgagtgt gtgtagctat tttttaatca
    13441 atattttagt aaattttacg gtaagtaaaa acctctctcc ttcaaatcct atagcaaata
    13501 tacttacaag ttgctttgag aaaataactc ctaatatagc tacaactatg catataccaa
    13561 ctattatatt taaaacattg tttaaaaact ttagtgcttg cttttcacct tgtttactac
    13621 ttatatcttg atacattgga ataaaagtag taactatagc agtacctatt gctgcaaata
    13681 taatatttgg tatattcata gctgttaaat aactctctgt atataagcct gtaccataaa
    13741 ttgatgataa tactaattct ctacccattc caagaatctt agagattata gtaactatca
    13801 tcaaataaaa agttgcttta gctaccttgc tcattttatc actccacttc taacatacct
    13861 ctttttaatc aagtaaaatt tagctcatta gatataattc tatcaactaa tttgccttgc
    13921 ttagccctat caaaatataa ttaacaccta tttcttaata tatcacaaac tacattattc
    13981 ttcatatttt tttatttctc caataagttt tacatttttc ctatataaat tgtatagctt
    14041 caattatatt caaagaaatg gagttgctac ccgataactt taaaagtaaa aaacagattt
    14101 tattatcttt ttaaactata ttctaacata ttttgtccat attgagaaat tttactaaac
    14161 tattatgtac tttattaaat aaataaaata acagcacacc tataaaatac aagtgtgctg
    14221 ttcataaaca gattttataa aaaaataatt cctaagtaga gattaacatg ctttctcata
    14281 ctctcatata taatgtgtct aaaaatatta ataaaaatgt tttaattgtt ttaacttact
    14341 tggatgtctt aattttctta ttgctttagc ttctatctgt cttattcgtt ctctggtaac
    14401 tccaaatacc tttccaatct gttctagtgt tttagggtca tcatcatcaa ttccaaatct
    14461 cattcttaaa acttgctgtt cttgctctcc aagacctgta agtagttctt ctatcttttc
    14521 ttttaaatta tgctgttcaa cttcgtgtat tacaacatct ttaaaatcag catctgatgg
    14581 tataaattca actaaactac tatcctcatc ttcttttaat ggagtatcca aagatacaac
    14641 atttttatct cttcttaata attccaacac tttgtcaact tctaagccac atgcatctgc
    14701 aatctcatct atagttggct ctcttaataa tacatttaaa agttcttgtt tctttttctt
    14761 tacaaaattt attctctgat gaagatgtat aggtatccta atagtatttt cgcaatcgtc
    14821 gatatatctt gttatacttt gttttatcca ccaagttgca taagtgctaa atttgtatcc
    14881 ttttttataa tcgtactttt ctacagcttt tataagtcct atatttcctg cttgaattaa
    14941 atccaacata tcaatactat ctcttttata tctctttgct atgctcacta ccaacctata
    15001 atttgaattt ataaattttt cttttgctac tgaagatgag ttgattattt ccttagccag
    15061 ctctacctct tctcctgctg acaacatttt gtattcctct atttccttta aatacatttt
    15121 catcgcattc gatacatttg atgaacccac acaaatataa ttttcataat tattttcttt
    15181 tttagtatcc ataatcaaac cccttctatt catagaattt gctaaaacaa aaataaataa
    15241 tttaaaccta cataatctac aattacataa tatcccattc tttaaatgaa ttgttttgta
    15301 tattatgtat acaaatttat aaaaactata cctataatat tattctactt ctaacagcaa
    15361 tatcctcctt atatatataa tttattttat tatatgttgt tatattctta gttttttgaa
    15421 tgatatatgt ctaaaaaaag actgtttact aaacagtctt tttttatata tatgctcaat
    15481 atttaatttt catgatgatg attacatcca catccacaat tttcatcatg gtgatgatgt
    15541 tcttctaaat cttttacctg ttgttccatt tcatgaaact cttttaccat tatatcataa
    15601 gttggaagag ctgcctcagt atcgtattct tcccctctcc aatcagcgta catcatagca
    15661 tctccattag ttaaaaccaa tctacctgac atagttatat tatccacttc ttctaaaaat
    15721 tctatttgtt tttgtaaaac ttctccttct aactccacat cttcatctaa acattgcact
    15781 atcattacag gtgcataata atcttcttca tctttaacat ttacaacaat atctaaaatt
    15841 ccttctgcac cttcttcaaa ttctgccaac tcaacatttg tatcaatcat atcttgaggt
    15901 actaataaat cttctattaa atattttatt actctatctt ttactaattc ttttgacatt
    15961 tatttttctc cttccgattc tataacatta tcataatcat ttctatatta tagtatacta
    16021 cattaattgt tttttcaatc tcttacaaaa attatttttt attatcttcc ttatattccc
    16081 ttttttatta caaagttgta atcgcattga tttaaaaaat agtttatatt tagtataata
    16141 gaaatgtagc acatattttt attcaatata ttaataaaaa aatatattga aaatgtaatg
    16201 tatgttaaat ctaggaggaa tattatgaag tttaaaaaac aaattagttc tattgttgtg
    16261 tctactatgc ttgtacttgg gagtatgaac ctttcttatg cagacggtac aaatgtagtt
    16321 acacttggag ctaacttatc agagtctcaa aagcagcaaa tgcttaatta ctttggagta
    16381 aaaaaagacc aagtcttagt tttagatgta acaaatgcag aagaaagaca gtatttacaa
    16441 ggtgttgcaa cagagggtca acttggaaaa gttactatct cttgctcata cgttgaacct
    16501 actaaaaaag gaaatggaat taatgttaaa acagcaaact taacttgggt aactagttca
    16561 atgattgctt caacccttac aacagctggt cttgaagatg caaatgttat tgctgctgct
    16621 ccatatcctg taagtggtac tggagcattg acaggaatca tgaaagcatt tgaggatgct
    16681 tcaggaaaga aacttgatga aactaagaaa gaaattgctt ctgaagaatt ggtagttaca
    16741 ggtgacttag gagatgatat agggcaagaa aaagcaacag gtgtaatgaa tgatataaaa
    16801 acagaaattg taaaaaatgg tacaaaagat accaatcaaa tagcaaatac aataaataat
    16861 gttgtaaata attacaatat tactattaca ccagaacaaa aagaacaaat taaaggtgta
    16921 atgaaaaaaa tagctgacca aaattacgat tacaatagca tgaaaaatac actagataat
    16981 gtatcagata atgtaaatga acaattaaag aaattaggtg aaagtgttaa gggttctggt
    17041 atactagata ctattggtgg atggtttagt ggaataggag actggttctc tggtttattc
    17101 tcaggcggag ataaaaaaga tttgggaatc ttaaataata ctaatgatga gtctcttggc
    17161 tctgatgctg taataaattc tacagaaggt gttcaaataa atccacaaaa taatactaat
    17221 tctaacaatg atagtgatac aaatagctca aaaaatgata cttcaagtaa taatgaaaat
    17281 aacgatagtt ctcaaacaaa tgaaaacaac acaacaaata attcagaaaa tcaatcaaat
    17341 aataatgact cagttggttt ctttgaaaaa ataaaaaatt ggctttcagg cttattttag
    17401 aaattaaaca aataaaaaga attgcctaaa atggaaattt tctataaaag gcaattctct
    17461 ttatttacaa taaattttta ttactcactc atttttttag atttttctat acatgcacat
    17521 attgctttta tcacagaagc tctaaaacct tcttcttcaa gtactttaac tgcttctata
    17581 gtagttccac caggtgaaca taccatatct tttaattcac caggatgttt tcctgtctca
    17641 agtaccattt ttgcagaacc cataacagcc tgtgatgcaa acttatatgc ttgtggtctt
    17701 ggcattcctg caattactgc tgcatctgct atagcttcta taaataaaaa tacatatgct
    17761 ggtgcagaac cacttgcacc aattacagct tctataagat actctggtac tacctcagta
    17821 tttccaaaag aattaaatac ttctgtaact gcttctaagt cttctttgtt tatattttta
    17881 tttatagaaa gagaagacat ggcttcattt acaagagctg gagtatttgg catagttcta
    17941 actatttttt tatcttctcc tataatagac tctatatctt ttatagattt tcctgctgct
    18001 attgtaacta taattttatt gtcatctata aagtctttta tctcttccaa aatatcatca
    18061 tatatatctg gtttaacagc tacaattact atatcagagg ttctagttac ttccttcgag
    18121 tcttttgtag tatttattcc aaaatttgca tctattctat caagagttcc ttgactatat
    18181 gctgatgcta taaccatatt aggttcaact aattttgaat ttactattcc ccctatcata
    18241 gcgcttgcca tatttcctgc acctatgaat ccaatttttt tcactttagt acctccttat
    18301 tgactaatag taatcgttaa aatttaagct tctttaactg catcagactt ttcaaatgga
    18361 tttggtatac ttactaccaa aattttcact accttattag tatagttgac ccaattatga
    18421 ttgttctttt cactattata gtgtattgtg tctcctggat acatttctat ttgttcatct
    18481 cccaaaaaca ctgtaagagt accctctaat acatacacaa attcctcacc ttcatgtacg
    18541 taacaacaaa tttcctcact actcttactt ggaagaattt caattaatct tggtagcatt
    18601 gttttttcct ttaaactaga tgataaatga taatgtataa atgttgagtt ttctacatca
    18661 aatacttctt tttcataact tcttaataca gctcttttat gctcttttgg cttcataaaa
    18721 aagtaagtta attctacatc tagtacactt gctatttttc ctaaggaatc tgttgccaca
    18781 cttgttaatc ctctttcaag ttgtgataaa aaaccaattg acaagtttgt tttttcactc
    18841 atatccttca gcgtcatttg cttttgagtt ctgagttgct ttatttttgc tcctatgtcc
    18901 ttattcaaaa ccatcacccc taaataattt taattttttc ataatataaa tacaaaaata
    18961 taaatatagg tactgaacct atctttattt catttaaaaa aatttttata agtattacta
    19021 taatttatca atcttttttt gtctacattt ttacgattat ttacgattta tttttcacat
    19081 atgtatattt aatcttgcca actaaagata cattttttat aagcatttac tctttatata
    19141 ataaaactta agttgttcat gagtcttctt ctggtttaat tattaaaatt tcaaattata
    19201 ttattatttc tacacattat tacatccttt agtacaatta taacataatt ttacaattta
    19261 atgatatcta t
//
LOCUS       DACULQ010000066        19254 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.066, whole genome shotgun sequence.
ACCESSION   DACULQ010000066 DACULQ010000000
VERSION     DACULQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.066"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(315..1514)
                     /locus_tag="JA154_09280"
     CDS             complement(315..1514)
                     /locus_tag="JA154_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4'-phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenoylcysteine synthetase"
                     /protein_id="HAT7996055.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(1519..1785)
                     /locus_tag="JA154_09285"
     CDS             complement(1519..1785)
                     /locus_tag="JA154_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAT7996056.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(1788..2405)
                     /locus_tag="JA154_09290"
     CDS             complement(1788..2405)
                     /locus_tag="JA154_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAT7996057.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(2435..3316)
                     /locus_tag="JA154_09295"
     CDS             complement(2435..3316)
                     /locus_tag="JA154_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996058.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(3320..4150)
                     /locus_tag="JA154_09300"
     CDS             complement(3320..4150)
                     /locus_tag="JA154_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAT7996059.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(4437..5471)
                     /locus_tag="JA154_09305"
     CDS             complement(4437..5471)
                     /locus_tag="JA154_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase"
                     /protein_id="HAT7996060.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            5767..7542
                     /locus_tag="JA154_09310"
     CDS             5767..7542
                     /locus_tag="JA154_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAT7996061.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDALHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(7757..9409)
                     /locus_tag="JA154_09315"
     CDS             complement(7757..9409)
                     /locus_tag="JA154_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAT7996062.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(9708..10919)
                     /locus_tag="JA154_09320"
     CDS             complement(9708..10919)
                     /locus_tag="JA154_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAT7996063.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(11203..11736)
                     /locus_tag="JA154_09325"
     CDS             complement(11203..11736)
                     /locus_tag="JA154_09325"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089112.1"
                     /note="regulates pyrimidine biosynthesis by binding to the
                     mRNA of the pyr genes, also has been shown to have uracil
                     phosphoribosyltransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase"
                     /protein_id="HAT7996064.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(12096..13007)
                     /locus_tag="JA154_09330"
     CDS             complement(12096..13007)
                     /locus_tag="JA154_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HAT7996065.1"
                     /translation="MDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRSYIQKIIKDGKVK
                     VNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDVLIINKPQDMVVH
                     PAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLMIAKNNYAHNFLA
                     EQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKDGKNAITHFEVVE
                     RFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKLKGQTLHAKKLGF
                     IHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(13012..13458)
                     /locus_tag="JA154_09335"
     CDS             complement(13012..13458)
                     /locus_tag="JA154_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089114.1"
                     /note="lipoprotein signal peptidase; integral membrane
                     protein that removes signal peptides from prolipoproteins
                     during lipoprotein biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAT7996066.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(13768..14547)
                     /locus_tag="JA154_09340"
     CDS             complement(13768..14547)
                     /locus_tag="JA154_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide deacetylase"
                     /protein_id="HAT7996067.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(14602..15084)
                     /locus_tag="JA154_09345"
     CDS             complement(14602..15084)
                     /locus_tag="JA154_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7996068.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(15350..16771)
                     /locus_tag="JA154_09350"
     CDS             complement(15350..16771)
                     /locus_tag="JA154_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HAT7996069.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(16917..17687)
                     /locus_tag="JA154_09355"
     CDS             complement(16917..17687)
                     /locus_tag="JA154_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996070.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(17692..18894)
                     /locus_tag="JA154_09360"
     CDS             complement(17692..18894)
                     /locus_tag="JA154_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996071.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         6323 a         3396 c         1958 g         7577 t
ORIGIN      
        1 tagtattact tctaactatt acctttgcat attttttcat tataaaacct ctacttaaat
       61 ttaatatatt gttcatattt taatatactt tacaagatat taaaatctca gaatttataa
      121 gcaatttcaa gttattaata ttaactttat tttaataatt aactattaat tatattttat
      181 actttatgac aaaataaatg ctgaaaacta caatttaact gacatttgcg agtttttact
      241 cgctaaaatt aaattgcagc tttcagccac tttagctgtg ataaaataca caaatatcat
      301 atttaaaaaa ggtcttattt ctcaagcaac gctttaactt tatctaaaat aacattggct
      361 acttcttctt ttttcatctt aggatactca gtaatatttc cctctacatc tattattttt
      421 acaatattag tatcagctct aaaaccagca ccttctttag ttaaatcatt tgccacaata
      481 aaatctaaat ttttcttttt tatttttcct ttggcatgtt ctataaggtc attagtttca
      541 gctgcaaatc caattagtat cttatcttct tttatttttc ctatttcata agcaatatca
      601 taatttctac ttagttctat tgataaatca tcattcttct ttttaatttt tttgtcagaa
      661 taagtttttg gtttataatc tgcaactgct gcactttgaa ttactatttt attttcactt
      721 aaatttgata ctactgcttc atacaaatct tgtgctgact ctatttgaac aaatttttta
      781 agattttgtg gaatagaaag atttgtagga cctgatatta atgttacatc agcacctcta
      841 gctatagctt ctcttgctat agaatatccc attttaccag aagacctatt agttaaatat
      901 cttactgggt ctatactctc tacagttgga ccagctgtaa ctatcatact ttgacctttt
      961 aaatcttgct ctttagtaag attagtcaaa acttcctcta ctattatatc tggattagct
     1021 agtttacctg ttccaatatc tccacatgca agccttccac tttccggttc aataaattta
     1081 tatccaaagc tctttaaagt ttgtatattt ttttgaacaa ttggattttc atacatatta
     1141 gtattcatag ctggtgctat aagaacctgt gcttttgttg ccataacagt agttgtcaac
     1201 atatcgtcag ctataccatt tgcaatcttg cctataacat ttgcacttgc tggcactatt
     1261 aagaataaat ctgctttttt tgctaatgat atatgttcaa cttcccaagt ttttggttct
     1321 tcaaacatat cttctactac ataattttga ctcagcgatt gaaatgtaag tggtttaaca
     1381 aattctgttg ctgattttgt cattataaca tggatatttg catttagttt ttttaactta
     1441 cttacaacat cacaagcctt atatgcagct ataccgccac ttatgccaat tacaacagtt
     1501 ttatctttta gcatgacctt actcctcttc tgaaatttgt tggtgttgct cagcatgatg
     1561 tctagcttct tctatctcta tttcctcttc tgtcaataat ctatatgtta tttttccact
     1621 tgcaacttct ttagttgcta cacatacagg cttttcctta gttttatttg aaacatatgg
     1681 ttcttctcca tcaattaatt ttcttgctct ctttgaaaca gtccccacta aatagtatct
     1741 gttgtctatt ttttctagta cctcattgat agatggtttt aacataatta taattcctcc
     1801 ttaaatttct ctattataat atttttatat ctgctaactt tattcttttc agatgatatt
     1861 ataccttcta tttctttagc agatgttttt acatcttcat taaatataaa ataatcataa
     1921 tccttaatca ttttaatttc ttcatatgca cagctaaatc tcttctctat ttcagcttct
     1981 gtttctgtac ctcttccaac aattctattt tttaattctt caagtgatgg tggtagtaca
     2041 aatataaata ctccctctgg gcaaacttct tttatttgtt ttgctccttg catttcaatc
     2101 tccagtaata catcttgtcc tttttctaaa cattccatta ttgccgcttt tggagttcca
     2161 taaaaattat cataaatttg agcatactcc aaaaactcac ctttttctat catctcttca
     2221 aacttttcct tacttatgaa gaaataattt actccatcaa cttctccagt tctgggtttt
     2281 cttgtagtag ctgatacaga aagttttatt gtatcatttt ctttaagtag ttctttgcaa
     2341 atagtacctt ttcctgcacc tgatggtcct gatacaacta gcaataatcc ttttttcaca
     2401 aacatttaag cattcccctt ctaatattgt attcttactc tatattttgt atttgttctc
     2461 ttattttttc caattcgctt tttacttcta ctactaaatt agttatattt atatcagatg
     2521 acttcgaacc tatagtgtta gtttctctat tcatctcttg tattaagaaa tctatttttc
     2581 tacctataga gtcgtcttta aatatagtat ttttaagttg ccctatatga cttttaaatc
     2641 taactatttc ttctgttata ctgcttttat ctgcatatat tgcaacttct tgtgctaatc
     2701 tattctcatc tattatactt gggtcatcca gtaattcact tattctcaaa ttcaattttt
     2761 ctctataatc ttctacaaca ctacttgaat atttttcaat ctcttctata tgatttttta
     2821 ataaatcaca tctcatggca atatcttcag ctaatttttt accttcttca cttctcattt
     2881 ctcttaattt gattaatgca tcttcaactg cttgattcaa cattgaccac aataaatctt
     2941 catcttcttc cttttcttct attttcacaa tatcaggaaa ctttgcaaca ttcataacgc
     3001 taatatcatc tataatatca aatttatttt ttatttcttt tagtatgtct atatactgag
     3061 atgctaatcc ctcatcaaaa tttaagttta catcttcttt gcctaataag tcaaacttta
     3121 tgtataaatc tactcttcct ctttttacat aattttttac taaatttcta actttatcct
     3181 ctaaaaatga aatttttctt ggaagacgta tgtttatatc tgaatactta tgatttatcg
     3241 ttttacactc tactaaaaag taataattat catccttgta ttctcctctt ccaaaaccag
     3301 tcatacttat agccattaat taaacctcca aaataccttc acatattttt actgctggac
     3361 ctgtcatata agagaaatct cctacatcta ttttaactgt tccaccttga gacgttacat
     3421 ttacactact ttctaccata ttaagtaaat tgcacactat tgctgatgca gtcataccag
     3481 taccacaacc taaagtatat ccacaacctc tttcccaagt ataaacatgt atattatact
     3541 tatcttctac ctttacaaaa tttacattcg ttttttcagg aaatatttta ttattttcaa
     3601 tctcttttcc atatttacaa acatcatcta catctaattc atctactata actactgtat
     3661 gaggaacccc catcaacata gaagtaactt taagctccct atctaaaacc tttatacttt
     3721 cttgcacaaa acgttcttta tttgtaagta caggaacgtc cttagctata aaacttcctt
     3781 ttcccatatt gactcttata gagtttattt ctctattagt aagatttact tttatatctt
     3841 ttataccatc taatgtatct actgtaaaac tttctttttt aactatacaa ttatcataaa
     3901 caaatttaca aaaacatcta aggccatttc cacacatagc tgctcttgaa ccatcagaat
     3961 tataatatac catttctaca tctgcaactt ttgaatcttt cacaactaac aatccatccg
     4021 ctcctacaga aaatcttcta tggcatactt tttttgctaa agcagtataa tcagatggat
     4081 ttatctggtc gaatcttcca tctattgcta taaaatcatt tcctattcca tgtagtttcc
     4141 aaaatttcat aagtatcgct ccttcgtaaa ttaacgctta aactattata acataaaaaa
     4201 attcccataa acttataatt tatgggaaaa gttttgtaat gacaactata atttgaggat
     4261 gtgagggaga gttgccatta caattagggt aattatatta tgtttttaag ctagttcttt
     4321 ctatgtttat atattaatat ttctatatga attttatgta tgttttattt aaactgtcta
     4381 tgaatttttt aattaaacat ctggtaacta gctataatcc ttctagtaga tatctattaa
     4441 catacaccta ttaattcttt aagtgttttt aaaacatagt ctaaatcttc cttagtaata
     4501 tctacatgag ttacaaatct atattcacca ttttccattt tattaatttt tatatttctt
     4561 tcaaaaaatt cttttattaa aacatcctct tttataatat cttcactcat cttaaaaaat
     4621 accatactta tatctggatt aacattgttt acttcaatgc catcaatttt atttaattca
     4681 caagctaaat attttgcatt ttcatggtct tcattcaatc ttaaagtcat tttttcaatg
     4741 gcaattatac cagctgctgc aactatacct acttgtctca ttcccccacc cataactttt
     4801 tgctttcttc tagcttttgc tataaattct ttatttccag caagtataga acccattggt
     4861 gcagctaaac cttttgacac acagaacata acactatcac aacactttgc tatttcttta
     4921 gcttctacac ccaaagttaa tgctgcgtta aataatcttg ctccatctaa atgtactggt
     4981 aagttatgtt tctcagctat ttttttaatt tctcttaaat tatcaagact cacaactgtt
     5041 ccacctgaat atgcattttc tacacatata agccctgtct gtggttcatg tatgtcatct
     5101 cttctaattg ccttttcaac aagttcagga tttagagctc cactttcgtc ttcatctata
     5161 gttctaagct gaactccagc tattacagca ggtgccccaa cctcatgagt cacaatgtga
     5221 taacctttac caataattat ttcttgccct cttaggcaat gagtaaatag agctaattga
     5281 ttcccaaaag ttccacttgg aaggaaaata gcatcttctt ttccaacttt agctgctgct
     5341 aattcttcaa gtttatttac agtagggtca tctctaaaga ttgaatctcc aacaggagcc
     5401 tttgccatag cttctctcat ttcgtcagta ggcatagtaa cagtatcact tcttaaatcg
     5461 ataaatctca ttttttcaac cttctttcat atttatatac tatagaacct taaaattgat
     5521 tttttctgta aagttcatat actttcatag cttatactct acacaataat tatactatat
     5581 ttactttata tttaatctac tttaaattat taattttaaa aataaagttt cctctctaat
     5641 aaatataaat aattatacaa tttaaatatt aatctctatt gtttaaattc cccctagtca
     5701 caaagcgtat attatgtgta taattaattt actgactcat tttggtcaaa gaaaggggtg
     5761 ctataaatgg cattagatgg attagttata cattcaatag tcgatgaatt atcatcaaaa
     5821 cttacaggtg gaaaaataga taagatacat caacctgaag atgatgaggt aatttttaat
     5881 ataagaaata ataaagaaaa ttttagattg gttttaagtg caagtgcctc aaatcctaga
     5941 gtttatctta caagtaatta tcaaaaagaa aaccctctaa aagcgcctat gttttgtatg
     6001 ttacttagaa aatacataca aggtggaaat atagtagaaa tttctcaaat aggttttgaa
     6061 agaataataa aaataagtgt tgaatcttta gatgaattaa aagaaaagac agttaaaaat
     6121 attatgattg aaattatggg aagacatagc aacataataa taactcatgg tgaagaaaat
     6181 aaaattatag attctataaa aagagtccct tttagcataa gtagagttcg tcaagtttta
     6241 cctggtcatg attattcatt gccaccagaa caaaacaaac tcaaccctct tgatgatata
     6301 tctaaggatt tgtttattaa aaacctagag gaattggaag gacctatctt taagtctata
     6361 tattctaaat ttctaggtat aagccctatt atagctaaag aaatttgcta cagagcaggt
     6421 gtaaatcaaa atgctatcat aaaggatata tctgatgaac aatttgatgc tttacacaaa
     6481 gtattttgta atttattcaa tgatataaat tctaacaaat actctccttg cattattata
     6541 gataaaaagg tggataaggt agttgatttc agttgtataa accttacatt atttagcgat
     6601 ttatcatata taaataagga tagtatgtct cgtattctgg aagatttcta tagaactaag
     6661 gatattaaag atagaattaa tcaacgttca tctgatttaa agaagagtat atctgttaag
     6721 cttgatagac tttataataa acttaaaaaa caagaagaag aattatcaga atccgaaaat
     6781 gcagacattt ataagataaa aggagaattg attacttcat atatttatat ggtagaaaaa
     6841 ggtatggaaa gtattgaagt tgctaacttc tatgatgaaa attgtaatga tgtaacaata
     6901 gaattaaata aaaacttgac accatctgaa aatgctcaaa aatactttaa aaagtataac
     6961 aaaatgaaac atgctaaagt cgagatatct catcaaattt ccttaaataa ggaagaaata
     7021 gattacttag aaaacataat tttaagtatt gaaaactgtg aaaatttagc tgaattacaa
     7081 gatataaaag aagaacttgc taaagttgga tacatcaaga ctcaaaagaa aaacagtaaa
     7141 aaggatacta tcccatctac aaaacctcat gaatttgtgt cttctgatgg atttaaaata
     7201 ttggttggta aaaacaataa gcaaaatgac tatcttacac taagacttgc tgataatgat
     7261 gacttatgga tgcatacaaa aaatattcct ggttctcatg taataataaa gtgtgctggt
     7321 aaggaagtcc ctgataatac tgtatttgaa ggagctatgc tagcagcatt ctttagtaag
     7381 tcaaaacttt cttctcaggt gcctgtagac tatactaaga gaaaaaatgt gaaaaaacct
     7441 agtggttcta aaccaggtat ggttatttat gaaactaaca gtactattta tgttactcct
     7501 gaagaagaaa cagtagccaa gcttaaggtt aaatctgaat aaattctgca ctaaatacaa
     7561 aataaatgtt agacaactac acattatcat tctaaataaa aatagtggcc ttcttataaa
     7621 gtacaataat aaggccactt tctaattagc ttacttttaa ttttctaaca aaatactttt
     7681 ctgttttaat aactatactt atattcccaa caaatatttt cagtcttctt atatagatgt
     7741 aattaaaaag ttaacactaa aagctatttt ttaatttatt gatttgtttt agtaattcat
     7801 tatattctga ttcatacaat tcttttttta gattatcttt ttctatagat aacatagata
     7861 taacagttgt aagtctattt tctaatacta gaagattttc ttttttgatt tggttttctc
     7921 tactaggagt ttttttattt ctgctaattg tatattcgtc ataattacaa tcaaactccc
     7981 ttattttagc atcttttatc tctataatat agttacaaat atgtgataca aatactctat
     8041 catgagacac tataagcata gttttatcag tatttatcag tgccttttcc agtgcttcca
     8101 tagattttat atctaagtaa tttgttggtt catcaaatac taaaaaatta ttatcttcca
     8161 atattatttt acatagtgct atttttacct tttctccacc actaagtact tttacttttt
     8221 tgtagacatc atcaccttta aacccaaata ggcttaagtt tatccttata aaagattcat
     8281 caaatgaact attaacttta gtattgtata gaacactctt ttcctcatct aataaacttt
     8341 gattttggtc aaaataacct acttttacct tgttatttat ttttatattg tcatttttat
     8401 cagccaatat ttcttttatc agagtacttt ttccacaccc attatcacct aacaatgcta
     8461 tctttttacc tctttttatc ttaaaggaaa cattatctaa caggagctta ttttctaact
     8521 ttagagtcat atcttttacc tctactagat ttttgctaat tatctccata ccatcttgca
     8581 tcttaatgtt catttcttta gaaactttag gtttattctt tacctcaagt ttatcaattc
     8641 tgctttttat agccttcaca tttgagtcca actttttctt attagtctga ccacccattt
     8701 tgtgcaacct agcttctgaa ttacccattc tttttggtgt ttttcttatg ccatcactta
     8761 aagctttttt atataaaaga gctttttcaa gccttttttt ctctttaaca tattcttgat
     8821 actcaaaatc agctcttttt acttcttcat ctttttgcat taaataatca gtgtaattgc
     8881 ctttgtaagc ttttagtttt ccatcttcta gttctaatat agttgtacat aactcatcca
     8941 gaaaacgtct atcatgagat attaataata atgctccctc atgcctttta aacatatctt
     9001 ccaaaacccc aatgcttttt ttatctaagt ttgaagttgg ctcatcagct attatgatat
     9061 ttttcttatt actcagcgca tctgctattt ttaacttaac cttttcccct cctgataagt
     9121 gttcttcaaa tttatctgga gcatttaaaa gactcttctc cttactatga ctacatgttt
     9181 caatattatt ttcactctga cttatataag aatagctttc tgtaaggtaa gtatatcctt
     9241 catcaatttc tatttctccc aatattgctt ttaataatgt tgtttttcct tgaccatttg
     9301 aaccaactag acctattttg tcattttcta atatttcaag tttatctata tccaaaatta
     9361 atttatctgc ataatatttc tttaaattct ctacttttac taacaacata aaaaatccct
     9421 ccttaacaca cttaaatata aggagagatt ctataaatag gcataacaaa taaacgtata
     9481 gactataatc tactctcctt aatttaagca ttgcaaataa agccaccaaa ataggctttt
     9541 caaaaattac aatactttaa attataaaaa atagattata ttctcattgt cctagttacc
     9601 ttgccctttc tttcatttat ttttaatcat tatatcaaca ttaattacaa atgtctatag
     9661 aaaattaact aattttagtt attcaatata caacttattc tttaaattta ttcttcttca
     9721 actttaaagt cctgattatt taaaattctg tttaatacaa ttccaacaat tgcagctaaa
     9781 ctaagaccag ttatctttac tgtacttgtt atagggattc caacagaaat acctttatta
     9841 gataaatatg ttgttccaag acctattaat aatataactg ctatgattaa tatatttatt
     9901 ttactttcta cacaagaact gtcccttata gtttttaatc ctacataagt tatcatcgaa
     9961 aatagcatta tactaattcc acccataact gaacctggta tactttgaag aaatccacca
    10021 aacttgccta cacatgcaag caatatagca aatattgctg tacgtctaag tattgaaggg
    10081 tcatagtttt tagttatagc aagtactgct gtattttctc cataagttgt attggctggt
    10141 cctcctaaac accctgcaac tatagtcgca aatccatccc caagtaatgt tctatttaat
    10201 ccgggatttt ctataaagtt tttacctact acagcaccat tagtagtcat atctcctata
    10261 tgttccataa atacagctaa tacaactgga gatataatca ctattgctcc taaactgaac
    10321 ttaggaactg taaagttagg tattgcaaat aaacttgctt ctgtaatagt agttatatct
    10381 actaatctta atatcaaaca gattatatac ccagaaaaaa ctgctattaa tattcctaat
    10441 tgttttataa atcctctacc aaacttattg gttaaaattg ctattgctaa agttataaat
    10501 gctatcacaa aattggctga agccatacta aaagctgttg gcaataaatt taatcctata
    10561 actactatca tagctcctgt aacttgtgca ggaaaatata atttaatttt atttacacca
    10621 actactttta ctataatcga cattataata taaattaatc ctgctactat tataccacct
    10681 tgagcatatg ctaaatctcc accatatgcc tctttagctg ctagtataac tggtataaac
    10741 gcaaaacttg aacctaaaaa ggcaggtact tttccttctg tacaaaaatg aaatatcaat
    10801 gtaccaacac cagcacagaa aattgcaact gacggattaa aacctgtcaa aatgggaact
    10861 aatactgtcg ctccaaacat ggctaataga tgttgtaaag acattatcgt ttttttcata
    10921 aaaacctcca aaattattat atataaataa atcctctgtt taattaatat cttttaatta
    10981 attatcattc cctagattct attttttaac ctctatatac ttattatata atttatcatt
    11041 attttacatt ttacgacatc aaaaactcaa ataaaggaga ataaaaacta cattaagact
    11101 cacatccata aaattataat atttattttg tgcaatctat gtaagcttta tcctcctccc
    11161 tgactattca atgctaagct tttatataat acttaaaaat aattattctt tgattgtaac
    11221 tgaatcttct ccatcaattt ctaataaact gacagatata atttcatgtc tagaagtagg
    11281 tacatttttt cctacataat ctgctcttat aggcaactct ctatgccctc tgtccactag
    11341 cactgctaat tgtattgatt taggtcttcc tatatctata atagcatcta gtgaagctct
    11401 cacagttctt ccagtgtata aaacatcatc aactaaaact actgtcttat catttacatt
    11461 aaaatctaaa taagttcctt taactacagg ttcaacatga attttttcca aatcatctct
    11521 atacaaagtt atatcaatat ctcctgtatc tacttttgtt ccttctattt gttctatttt
    11581 cttagaaatt ctatttgcaa ttggcacacc tctagtcttt attccaacta aaactagatt
    11641 ttctacacct ttatttcttt caattatttc atgacttatt ctagtaattg ctcttgctat
    11701 tgccttttca tccattaatt gagctttttc taccatatca taattattgt caaagatata
    11761 atatatctat ttgacaaatc tactcctttc ttttaatttt ttataaattt atttttattt
    11821 aaaatttcaa tttaaacaac aaaaaaactt ctcatcacaa gacaagaagc tcttatataa
    11881 aaaatataca gtgcaattta gcaattgtct tacgatatct ctgtatcgtt taaagataat
    11941 tttttaatct atcaaatcaa attatatatt tatatttcaa atttttcaag cttttttaaa
    12001 caaaatactt taatataaga tattttattt aaatttcact aaatttattt acttatataa
    12061 aaacaaaaaa tgatttataa gtattataca aatagttatt ttatcttttt aataacttcc
    12121 ttaaaatact caggtaattc tgaattaaat tcgatatatt ccttagtagt aggatgtata
    12181 aatcccaact tcttagcatg caaagtttgt ccttttaatt taaatttagt ttcttttgga
    12241 ccatatacat aatcacctaa aagtgggtga tttatagaca aagcatgaac tcttatttga
    12301 tgagttctac cagtttcaag cctagctctc atatgtgtaa acttatcaaa tctttctaca
    12361 acttcaaagt gagtgattgc attttttcca tcttttacta cagccatttt taatctatcc
    12421 ttaggatttc tccctatagg tttatcaaca gttattttat cttctttaac cacaccatag
    12481 catataaatt catactccct agttatagta tgttccttta actgttctgc tagaaaatta
    12541 tgtgcataat tgttcttagc tatcataaga agaccagaag tatctttgtc tattctatga
    12601 actataccag gtctaataac gccatttatt gatgacaact tatctttaca atgatataaa
    12661 atcccgttta ctaacgtatt ttcatagttt ccaggagcag gatggacaac catatcctga
    12721 ggtttattta ttattaagac atcatcatct tcatatacaa tatctattgg aatatcttga
    12781 ggtaaaactt ctaatacttt aggttctggt atctctataa caatattatc ttcctcttta
    12841 accagatatc tagctttttc tactttatta ttaaccttta cttttccatc ttttataatt
    12901 ttttgtatat aacttcttga catatcacct aattgttctg ctagataaat atcaagtcta
    12961 acttcttctt cctcttcaag tactaaaaac tcttttactt catccattat atcacctact
    13021 tttactttca aaaaataaaa catatataca taaaaacaca gttcctacaa ctacaaatac
    13081 atcagctata ttgaatacat attcccatat aattctaaag tcgaagtaat ctactacaaa
    13141 acctaatcgt actctatcaa ttagatttcc caatgcacca gatataatta atataattcc
    13201 caaccttcca aatatatgta ctttccttgt attaagatag tatagtccaa atactgttgc
    13261 aagtaatgca acaattataa atatccattg attattttgt aataatccaa atgctgcacc
    13321 tctattttcg acataagtta aatgaaatac attatttatt attggtattg ttgatacatc
    13381 caccaaatta ttcaataccc atattttaga cagttggtct aaacctatga gtagaattat
    13441 tattaatata tatagcaatg atttttcccc ctataaatta tttaaaaacc aattttattt
    13501 gaagcacact attattatta tacaaaaaaa atgctctaaa gagcaacttt attaaaattg
    13561 tattttctta ataaagctag aacaaaattt aaaatagtac tacttttttt atattgtaga
    13621 tttctacata tttaaaatca tatcattatg tataaaaaat ttaaaaatac catactaatc
    13681 aatatttttt gtgtatttta tagattaaag attaatgatg tctgtaattt aaacttacaa
    13741 tcaccactaa tcttcaaaca aatattttta cttttgcact tgcttatttt tatctaaatg
    13801 ttgtataagt tcagatactg ttacgaattt atatcctctc tcttttaatt taggaattac
    13861 tacttctaaa gcttgtatag tttgtgattt atcatttctt ttagtgttat aatcatgtaa
    13921 aagtattata ttaccatttt ggacctttgt aaggatagta tttataattg catctactcc
    13981 agggttagac caatctctag ggtctaagtt tgaccaaaga attatattca tgcctttaga
    14041 aactataata tcacagacac ttttactcat ggctctatat ggaggtctaa atatctttgg
    14101 ttctactcct attacacttt ttataatatt ttgcgtatct gttatttctt tttcaacact
    14161 actataacta ttttttgcaa catttatatg tgtaaatgta tgattcccta tttcatgccc
    14221 ctcatcatat tgtcttttta ataattcttt gttatattct actttttctc caactacaaa
    14281 aaaagttgct tttacatcat ttttctttaa tatatcaagt acttgtggtg taaaatcctc
    14341 atctggacca tcatcaaaag ttaatgctaa aattttttca tcctcatttc ctttttttat
    14401 tattacatct tcataatcgt cttttatatt gtcatttaaa ctatatgctt cttttgaact
    14461 atcttgatta tgtgtattat gattatatgt atatacacct actatagata ataaacatat
    14521 acatataccc catattattt tcttcatgtc attacctctt ttctaattca ttctagcaat
    14581 atattttata atgtcagttt tttaatctct caatattcca tttttattta aatcagttaa
    14641 atttgttagg tcttctgaat aaaagttaga atcttctaca tcagggtttt gcattgcatc
    14701 acctaataaa ttattttctt tctttgcaca atcattacat aaacttgtat ctggcaatat
    14761 atccaatctt tctaaatcta tttgattatg acataaatca caaatcccat atgttccatt
    14821 ctctatttta gataatgcta agtctatttg atacaatctt ccctcttcat gatttattag
    14881 actgttttgc atattttgca tatataaatc tgttcccatg tctcctatat gattatcata
    14941 actagataat tctcctgatg aatacttttc acttgtatgt ttagttgtat ctccaaataa
    15001 agtattatct ttcatatcct ctactaaatt agttaaattt tctctttctt tcaataattt
    15061 atctttatat tttttagtat ccatttttaa cacctctaat tttaatattt acatcataaa
    15121 aatagtatgt ggaaaagtaa attaaacata caataaaaat aagatttgat tcgaaaaatg
    15181 gaatgtctca aaatgaaatt tttagttcat ttaaccctct tttttatatg aaatcaaata
    15241 tatttttatc ctttcagcaa taaaaaagag acttatctct attttgagat agtctcttct
    15301 ttgaacataa tttaaataca attttttaat ttttatagtt attattttac taacttgcca
    15361 atttatgatt attttgttct ttaatatgtt ttgctttttt aaagaataaa agaccaagaa
    15421 ttattgctac aataacacct attccattag atatattaac tggtagtcta aatccttctt
    15481 ttgcttgcat tatataacta aatgttacta cagtcataaa aagtgctggt aatgtagtta
    15541 tataatggtt tttatttgct ttgactaaat atgcagaacc agtccacaac attatcattg
    15601 caagtgtttg atttgcccaa gagaaatatc tccaaattat attaaaatct ataaatgtaa
    15661 gagcaacacc aactgcgaat aatggtaaag ctattaaaaa tctatttttg actgcatctt
    15721 gattataatt cattgcatct gctattgtca gcctagcact tctaaatgct gtatctccag
    15781 aagttatagg acatgccaca actccaagta tagctaaagc tcctccaata ggccccatta
    15841 atccaacaga tactgtattt actactgttg ctgctccacc atcattaaat atcacattaa
    15901 gttgaggtat gccatggaaa tatgacatgg ctgctgctgc ccaaactaaa gctattatac
    15961 cttctgcaac cattgaccca taaaatactg gtcttgcctc actctctttc tcaacacatc
    16021 tcgccataag tggtgattga gttgcatgaa aacctgatat tgctccacat gctattgtta
    16081 taaataagta tgggaataaa ggtgttccat ttggattcat attttgtaaa tttatatttg
    16141 gtatgttata cccctttatt attaatcctc cagctacacc tactgccata attaataatg
    16201 ctgctccaaa taaaggatat attttaccaa tcaatttatc tattggtaaa acagtagcca
    16261 taatataata aagtattatc actccaaccc atatttgtgc gtttacacct gtcaatgtct
    16321 ttaataatcc tgctggacct gttacaaata caactccaac caatactaaa agaataacac
    16381 taaatattct cattgctatt tttgcaccat ttcctagata aaggcctacc aactctgatg
    16441 cagatgtacc tttgtttttc attgatagat atcctgaagc aaaatcgtgt actccaccag
    16501 caaagattgt tccaaacaca atccataaaa atgctgatgg tccaaataat gctccttgta
    16561 tagctccaaa tatcggacct aaccctgcta tatttagaaa ctgaattaaa aagattctct
    16621 tccatttcat tggcacataa tctactccat ccctacaagc tatagctgga gtttgatttt
    16681 tgtcattggt tccaaaaacc ttttctacaa aagtaccata gacaaaataa cctactataa
    16741 gtactactat tgaacctaaa aagcttacca taaattcatc cccttagttt ttatataatt
    16801 gctttataaa tttttatgag tttaagaaaa ctatttccag ttcaaatagt aatacttttt
    16861 tatgctaata taaatattaa ttacattaag tgcatattta agtacatgaa atgactttat
    16921 atatgcatta acagcctaaa ttcttttact ttgcttctac ttacagttac ttcataatca
    16981 ctatttttta attttaatat gtatgtatta ttaaaccatg gtataatttc ttttactttg
    17041 gttaagttta cgatataaga cctatgtgtt ctaaaaaatg ttttatcatt tataagattt
    17101 tcgacttctg atatgccttc ttttaatata aacttttctt tctcagtata tataaatgtc
    17161 tgcctttcat tagcttcaca gtaatatata tcatcaatat ctattacaac taatttatca
    17221 cctttccaaa ggctaatttt atgtgttatt tcttctatct cctgatttac tgcttccttc
    17281 ttatatttgt ataaattact atttacatta aatagttcaa tatcactcat ctcacttttt
    17341 tctaatttat tcagcataga aataattctc tcttcagaat atggtttcaa tatataatca
    17401 aatacatcca aagaaaaagc atctactgca taactttcat atgctgttat aaaaacaatt
    17461 ttaggtcttg atttaaactg atttaatgtt tttgcaagta gcattccatc aagatgaggt
    17521 atatttatat ctaaaaaaat tacatctatt ttattttcct gaataaagtc taacacctct
    17581 ataccattgg taaattcact tactacctcg attccacttt tattttcaat aaaatatctc
    17641 aattcctttc ttgctggaaa ttcatcttcc actattatag ctctcatact tctactcctt
    17701 tacataaaat actatcaaag tcccattatc tattcttctt atattaagcc ctcttccata
    17761 catcagttta agccttaaat gtacattata aagcccaatc ttattttctg gcatattatc
    17821 ttgatataca ttatttataa tttcctcact tatacctata ccattatcct ctattgatat
    17881 tctcacagta ttttctaatg aatacggtaa cttttttatg gatattttca ctacaccagc
    17941 tctaccactt tctaaaatac catgtattat tgcattttcc actaatggtt gtataatcaa
    18001 acttggaatc tttatattta catcttctat atcataagat acagttagta attctccaaa
    18061 cctagctttt tctatctcta caaaagattt aacttgttca atctctttat ttatatcaat
    18121 taagttatca ctatattcaa gattatatct tagataagtt gataaattta ttattaactc
    18181 tctagcctta tctggactaa atcttataaa tgaagctatt gtgtttaggg aattaaacaa
    18241 aaaatgtgga ttaatttgtc tttgaagtgc ttttatttct gctttatttc tcgcttctat
    18301 atctttttta tctttctcaa tcccctctac tatagatata acaaatccta ttccaatctg
    18361 cccaactatt aaaggtaaat atattccacc gattatttga attgctaaag aatgtggata
    18421 tgatattaag tatataagta atatagtaaa actttcagaa atcatgccta ccaatattcc
    18481 atacattact ctatgttgtt ttggtatcct cttatataga aatcctgata atacacctgc
    18541 tagtatactt gataaaagac atggaacaga tgttatcccc ccaatatcca taaaatatct
    18601 atgaatccct gaaaaaactc ctgctgtaat acctaccatc ggaccaaata aaatgccacc
    18661 tgatactata gatattattc tagtgtttac tattgacccc atataattta taccactata
    18721 agttccaaat atggctaaaa aagtaaaaac taatgctata cagaccaagt cattaaaact
    18781 atattcttcc ttttgaaata tctgcttaaa tatttttaac tttgttatta gaagaaacaa
    18841 tactacaata aggcttgtct tttctattaa tgcgattaaa attgatgttt tcataatctc
    18901 cctttaaaaa ataaattttt acacactcta aatttattat ggttgttata aattacatat
    18961 ttgaagtaat cctatcacaa aactttccac atttaatctt ttaattaaat aagaaatagt
    19021 tgtacttcta ttaacaacta atactagcta gcataagctt gtaataactt gtagttatta
    19081 ttaactttac aattctagta tttaatttta tactttagaa atctcttttc ttagaaataa
    19141 tactttcgaa tatcatataa tacaatatta tgtagattct tactcataca acatatttct
    19201 ttaaaaattt tatactattc tttgttcaaa tatctcattt gatttaattg tgtt
//
LOCUS       DACULQ010000067        19225 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.067, whole genome shotgun sequence.
ACCESSION   DACULQ010000067 DACULQ010000000
VERSION     DACULQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.067"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..961)
                     /locus_tag="JA154_09365"
     CDS             complement(<1..961)
                     /locus_tag="JA154_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996072.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTIN"
     gene            complement(948..1715)
                     /locus_tag="JA154_09370"
     CDS             complement(948..1715)
                     /locus_tag="JA154_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacitracin ABC transporter ATP-binding protein"
                     /protein_id="HAT7996073.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            2003..2677
                     /locus_tag="JA154_09375"
     CDS             2003..2677
                     /locus_tag="JA154_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996074.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            2701..3705
                     /locus_tag="JA154_09380"
     CDS             2701..3705
                     /locus_tag="JA154_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996075.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            3817..5046
                     /locus_tag="JA154_09385"
     CDS             3817..5046
                     /locus_tag="JA154_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAT7996076.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            5054..5728
                     /locus_tag="JA154_09390"
     CDS             5054..5728
                     /locus_tag="JA154_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996077.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            5731..6963
                     /locus_tag="JA154_09395"
     CDS             5731..6963
                     /locus_tag="JA154_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996078.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(7249..8514)
                     /locus_tag="JA154_09400"
     CDS             complement(7249..8514)
                     /locus_tag="JA154_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAT7996079.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(8567..9715)
                     /locus_tag="JA154_09405"
     CDS             complement(8567..9715)
                     /locus_tag="JA154_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HAT7996080.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNKVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(9815..10360)
                     /locus_tag="JA154_09410"
     CDS             complement(9815..10360)
                     /locus_tag="JA154_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HAT7996081.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(10583..11497)
                     /locus_tag="JA154_09415"
     CDS             complement(10583..11497)
                     /locus_tag="JA154_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAT7996082.1"
                     /translation="MKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTPLQIITLRFFL
                     AAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTPSKNAFITAAN
                     VVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFLTLICSFGFAF
                     HIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGYMGTMYLAVFS
                     TTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLVIGSILIFVAI
                     ITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            11913..12794
                     /locus_tag="JA154_09420"
     CDS             11913..12794
                     /locus_tag="JA154_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HAT7996083.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(12855..14063)
                     /locus_tag="JA154_09425"
     CDS             complement(12855..14063)
                     /locus_tag="JA154_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAT7996084.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(14136..14388)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14540..15508)
                     /locus_tag="JA154_09430"
     CDS             complement(14540..15508)
                     /locus_tag="JA154_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HAT7996085.1"
                     /translation="MNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDFIVPVFFC
                     IICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINKPTMVMDD
                     LVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLMIALFPFG
                     MVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICVSYLAIFE
                     VLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYARILFLII
                     ILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            15681..16121
                     /locus_tag="JA154_09435"
     CDS             15681..16121
                     /locus_tag="JA154_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7996086.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            16448..16675
                     /locus_tag="JA154_09440"
     CDS             16448..16675
                     /locus_tag="JA154_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAT7996087.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            16701..18821
                     /locus_tag="JA154_09445"
     CDS             16701..18821
                     /locus_tag="JA154_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HAT7996088.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(18954..>19225)
                     /locus_tag="JA154_09450"
     CDS             complement(18954..>19225)
                     /locus_tag="JA154_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7996089.1"
                     /translation="YLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIGKYEDSYKSY
                     EKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
BASE COUNT         6848 a         2896 c         2482 g         6999 t
ORIGIN      
        1 aatttatggt ataactgtat ttataatttt cacttatgtt cctatctata tcgtaataaa
       61 gtgatatggt aaaccctagc atagttacac ttgtagctat taatattgct attacagcca
      121 atacactact attgctcttt aatttaaatc caagttcaga aaatgcaatt aaattctgtc
      181 ccttataata aaaacttttt ctcttcttaa cagcatcaat aatcatatac atagttgatg
      241 aaaaaaataa aaaagttcca ggtattattg taactaatat aaaaactgaa agcataatat
      301 tttcagcaat aaaataacta attgacatta aataagaaat agctattaat aaaacaccaa
      361 gtacacattt taactttata aattttttaa acttatactt ttctggatat ttattaaata
      421 actctataat tctcttattt ctgataacta tagtatttct tattccaata actatgaata
      481 ttattaaaaa taccgctagt gtctgaacta aggcctttat acttaagttc atatttacta
      541 ctatcatttc attcattaat tttacaagca tcatagtcat aagctttgaa aataacagtc
      601 ctaaaaatat acctattagt attgcaacta tacccatcaa aaaagtttca tataaaaata
      661 tttttcctat ctgatttttc ctcattccaa gtaaactata agttcctact tctcgcctac
      721 gtctattgaa aaaaaatgag tttgaataat atataaataa aaatacaaat accactatta
      781 caattgaact tgtattaata ctcatcctag ttttctctcc aagtgaactc aacgcttcat
      841 tatactctat agatttaaaa ctaaaaaata caaatacact aaatacaatt gaaataaagt
      901 acattaaata attaaaaaaa ctattcttta cattttgcat tgcaattcta aataaagtca
      961 tttttattaa ctcctccaat taaggaaact gtatttacta ttttattata gaactctctg
     1021 ttactttctc cctttacaat ttctgtgaat attattccat cctttataaa gagtattcta
     1081 ttgcaaaaac tcgcacttat agcatcatga gttaccatca atattgtttt attgctgtct
     1141 ttgtttaatt ttaatagaca attcaacaaa tccctagcag atttagaatc taatgctcct
     1201 gtaggctcat ctgcaagtac taatgatggt tttgttgcta tagctctaca tacagctcct
     1261 ctttgctttt gtccaccaga aacttcgcca gggtatttat ctaatataga ttctatattt
     1321 aattcttttg aaatctcttt tattctattt tctattactg atactctttc attttttaaa
     1381 gaaagaggca acattatatt ttcttttatt gtaagtgtat ccaaaagatt agaatcttga
     1441 aatacaaagc ctagattatc acgtctaaaa tctgccaatt cattttgctt cattttaata
     1501 gtattctttc catttatata aatatctcct gatgaaggta aatctattgt agaaataata
     1561 tttaaaagtg ttgatttacc agcaccggaa gaccccataa taccaacaaa ttctccttta
     1621 taaaccttga aagttattcc tcttagagct tcatgttttg aaccttttct tccatactcc
     1681 ttttttatat tttcaacaga taatatttct tccatagtta ataatcacct ttcatcacaa
     1741 ttagttattt atcaattctc acaaatatat tatatatatt aaaatggctt ttaaacattt
     1801 aattatatta atttaaggtt acattttcgt aagtattaat tatatttctt caaatttatt
     1861 gcaattatgt ttaaaatata atttatcaag taaaatatag ttatattaat ataattattt
     1921 taggtaataa ggtagaaata ttttaattta tcaagtagaa tatagttatg ttaatataac
     1981 tattttgaat taggaggtaa gaatgtttaa aataatggta gttgaagatg acgtctcatt
     2041 gaaaaatatc atagcaaagt gtcttactaa atggggacat gatgttcatc aaatagaaaa
     2101 tcttgaaaat ataatagaag agttcaaaaa ctataaccca gaattagttt tattggacat
     2161 aaaccttcca ttttatgatg gttttcattg gtgtaatgaa ataagaaaaa tttcaaaagt
     2221 tcctataatt tttatttctt caagaaattc taatatggat gtaataatgg gtgtaaattt
     2281 aggtgcagat gattatatac aaaaaccttt ttctgtagat gtcttagtag ctaaagttaa
     2341 tgccctttta agaagaactt ataattttgt tgataataac tcaaatcaga ttattcacaa
     2401 tggtgttact ttagatttat caactgcaac aataaactat gaagataata ctattgagct
     2461 aacaaaaaat gaaatcaaaa ttctccatga gcttatgaaa tataaggggc agatagtaag
     2521 taggaataaa ctcatgaaaa aattatggga taatgattgg tttgtagatg ataatacatt
     2581 aactgtcaat gtaaatagaa tacgctcaaa attaaatgaa attggtttag aagattttat
     2641 agaaacaaaa agaggattgg gttatataat cagttaaaac aattctaagt aggtgaattt
     2701 atgagcttta aagattttct caaggataaa ctaggttttt tagtatacaa ttttactttc
     2761 ttaattttta cagtatcagt tattatattt tctcctgtag atgtttttct gactgatact
     2821 attttatata tattagttgt aaatgttgtg tttttatttt tatatctatt tattagctac
     2881 atcaagaaga acaaattttt agataagata aaaaatgata cattcaaaat taatatgaat
     2941 gatatagaat tagctagatg taaaaatgaa gaaaaaatat acttgaacat tattaaaaat
     3001 catcaatatc aatgtaataa cataataaat aataatatcg agaaatttga agaaaacaaa
     3061 gagattttaa atatgtgggt acatgatgta aagataccta tagctataat aaaactcatt
     3121 cttgaacaaa atgaaaattt aattgatgaa aaaatttcag atgacataga tagtgaaata
     3181 tttagaattg aaaactccgt tgaaaaaatt ttatatttat caagactcga agattttcat
     3241 aaggattttt taattcaaga ggtaaatatt gaaaaaatca ttagaaatat tataaaaaaa
     3301 tactcaaaat attttatttc aaaaaaaatt attttagact taaataattt gaattttaag
     3361 gttttatctg atgaaaaatg gctatacttc atattcgagc aattattgag taattcccta
     3421 aaatactcct cattaggaga taatatatct atctattgca aaagaaccaa aaattattta
     3481 caattaagag tggaagatac tggttgtggt ataaaaaagg aagatttaaa tcgaatattc
     3541 aataagggat ttacaggtaa taatggaaga aacaattcta aatctacagg tcttgggctt
     3601 tatttagtaa aagaactttg tgataagctt ggtcataaaa ttgatgtaga ttcagagtac
     3661 aaccaatata caaaatttac tattacattc aaatgtgatt tatagtatct tattattgat
     3721 agtttatttt cattaactta tattcaactt cttatagtaa ttctatatgt acatcccagt
     3781 atataattaa gtagttttta taaaaaggag gaagacatgt cttttctaaa aaaatttaaa
     3841 aataaaagat ttagaaataa cagttttaaa agtaacaact ttagaaatag cagttttgga
     3901 agtagtggct tcaagtctaa aaataaaggt tttaaaaaca atccctttaa aaataagaga
     3961 ttcaaaatat atgcaggaat agcagtaatc atacttttaa ttctaggagt tcttgcttat
     4021 gttgattcga aaaacactga attacaagct aatgaaaatt ttatcgaaac atatactatt
     4081 cctgaaaatg aaaaaatatt tataaatggg atggttgttc ctaagcaaac taaggacttc
     4141 aatatatctg gagattatga gttaagtgat gttaatgtta caaatggtca aaaagtaaat
     4201 caaggagacc ttctttttac tgctaaaaat ccaactataa tagcagaaat agatagttta
     4261 aaatcacaac ttagtcaata caaaaaacaa aaaatatcac tttctgatat tgctgaaaat
     4321 aaagatgcta ttgcttctat aaatgcacag ataactgctt taaactctca gatagcttct
     4381 ctggataaaa aagcttatga cagagttact gctccttttg atggcacagt atacttgaat
     4441 gaccaaactg gaaatccaga ccaaccagtt tcatttatga ctattcaagg tctagagttt
     4501 tatatgaaag gacaagcaag tgaacaagat ttacctaaaa tgaagattga ccaaatggtc
     4561 aatatactag ttttttcaac tgaccaaaag cttactggta gaataagttt tatatctgat
     4621 aaaccatcta ctccaaatac tgaaatggga gctcagcaga atacactatc ttattatgat
     4681 ataaacatag catttgataa tcaagaagga ttagtaaatg gattccatct tcaagcatca
     4741 cttgaagtag ctaattcaag ctttaaaata ccagcatctt gtgtacttaa agacaacaaa
     4801 cactcatatg tatttaagga tttagatggt atattgaaaa agcaaattgt agatgtagca
     4861 agtcaaaatg atgattttgc tgtagttaga ggcggtcttg aacaaggcga tataataata
     4921 aaacacccta ctaaagaaat gaaagaagga gacccagttc aaggcgatgg cgtaactgct
     4981 ggttctaata atggagatac tactccaaac aaaaaagtag aagaagttac tatggatgtg
     5041 aattaattta catatgttaa ttaaattaga aaatattcaa aaatattaca aagtagggaa
     5101 agatgaatta catgttttaa aatccttaaa tttagaaatt gaatctggag aatttgtcat
     5161 gataatgggt aagtctggta gtggtaaaac tactttactt aatatcttag gttttctaga
     5221 tgtatttgat gaaggaagat atatatttga tggaacagat gtaactaatt tgagtgaaaa
     5281 tgaacgttct gtttttagaa atattaatat tggatttgtt ttccaacaat ttaatttaat
     5341 agaaactcta aatgtatatc aaaatgtaga gttgcctcta atttacaata aagcactaaa
     5401 aaaatctaat agagaagaaa ttgttaaaga taaattaagt tctgttgggc ttttggacaa
     5461 gcttaagcag aaaccacttc aattatctgg tggtcaacaa cagcgtgtag ccattgcacg
     5521 ttgcctagca aatgaccctc agataatctt tgctgacgaa ccgactgggg cattagatag
     5581 tgaaactagc agagaaatta tggagctttt aacaaggctc aacaaacaag gtaagactat
     5641 aataatggtt acacatgatc aagatttaac taagtatgca actaaggtta tacgacttaa
     5701 agatggtgtg tttacttcgg aggtgtaatc atgagtttat taaaaaattc tttggccaat
     5761 ttaaaaggtc acaagcttcg tgtatttgta gctttactct ggattataat aggaataact
     5821 tcagtaatac ttgttagttc tataggtaat ggatttcaaa aagaaattaa gaaatctgta
     5881 aacaatgtaa accctaataa aactactatt tcgtttgaat cagccgataa cacaggtctt
     5941 actgatgata tgagtatttt tttaaaaccc tttaatgcaa aagatttaga agaattatca
     6001 tttgttgaag gagtagaaag aatagctcct tcaagagatg ggtttaacct tgattcagtt
     6061 tactcttcac aagcatcttt tgataaaaaa actacgtatg ttgatgttgg accagtaaaa
     6121 aaagattcta aaataaattt aatttgtggt agagattttt ctttagatga tgaaaaaaga
     6181 aaagtaattt tacttacatt acaaagtaca agtgaaattt ttgaaaatcc agaagatgcc
     6241 ttaggtcatg gtatcaacat aaatggaact atatttgaaa taataggggt attagatgat
     6301 tctcagcaaa accaagctgg aggattcttt ggaggttacc aagatatgca atttactact
     6361 tccctagtac ctaaaaaggc ttttgacact ttaatgagtc aaaattctta ttcaaatgaa
     6421 atttaccagt tagacttagt ttcttctaaa ggctataatg taaatgaagt tgcaaacaat
     6481 gttatagcta aattatatga gatgcaccct ggtatcaatg gttcttatac tacaccagac
     6541 ccaactgaac agacagctta tcttgaaagc attaattcca atgtaaacaa atatgtatct
     6601 ataataactg tagttgcaat gtttgttggt ggtattggtg taatgaatat aatgtatgta
     6661 tctgttatgg aaagacaaag agaaattggt ataagacgtg ctattggagc aaaacctagg
     6721 tctatattat ttcaattttt agttgaagct gtttttataa cagtatgtgg tggaatttta
     6781 ggaactatag ttggatttgc tgctactaac tatgtttcta aatatatagg ttttgaagct
     6841 atacctagct taaatagctt attttatgca attgtagcta ctatattgac tggagttgta
     6901 tttggtctga ttccagcctt taaagcttct aaactagacc ctataaaggc aatttataag
     6961 tagaaattgt ataaagattt tcgattttta gattagttta tataaaacat acttaaatat
     7021 ataacgcact attatatatg atagattagg aacttaatct ttccaaatat aatagtgcgt
     7081 tctttcataa aaaggattct cttccttact atatctaaat taccaagatg ttatgttcta
     7141 attgttcaaa ctaatacttc caattttgat taatcaatct cattctccta atatacaatc
     7201 ttttactttc aatcattgta cactccgcct taaaaaaata atcttaaatt aggcgttata
     7261 tacaatctcc ccattacata ttgtataatt aacctttcca tataaagatt tacctttaaa
     7321 tggtgaatta tcagctttag aaacaaagct ttcaactgtc cattcttctt ctggattgaa
     7381 tataacaatg tctgctacag ccccttcttc tataaatcca atatttaaat tgtacaattt
     7441 tgcaggattt atagacattt tttctattaa ctgcatcata ctaagatgat ttttatgcac
     7501 caaatttgtt attccaagag ctaaagaagt ttcaagccca ataattccac ttggagcttt
     7561 tataaactct ctatcttttt cttctttact atgaggtgca tggtctgttg ctattatttc
     7621 aatagtacca tcatttaatc cctctataat cttatctctg tcccatttac ttcttagagg
     7681 tggattcatt tttgcatttg ttccaaactc caatacatct tcctcagtca atgtaaagtg
     7741 atgtggtgaa gcttctgcaa ctacatttgc acctaagtat tttgcaaatc taaccatatc
     7801 aacagataca cctgaactta tatgttgtat atcaacttta gcaccagtct ttagtgccaa
     7861 catacaatct ctggctacca taacatcttc tgctacactt gatgcacctt ttaatccaag
     7921 ttctgaagct acctttcctg catttacacc tggatttcca actaaagaag ggtcttcttc
     7981 atgaaaacta agtggtacat ccaactcttt tgccttaatc attgcctcca taattagatt
     8041 gctatccatt aaaggtaatc catcatcagt aaaccctggt acaccagctt ttttaagagc
     8101 ttccatatca actaactctt taccttcaaa acctttagtt atagctgctg cttgtaaaac
     8161 atttatacat gcattttttg acttttcttt aacataatta aaagtatctt cattatctac
     8221 aataggatta gtatttgcca tacatataac agttgtaaat ccacctctag cagcagattt
     8281 agctccactt tctatatctt ctttatatgt aaacccaggg tctctaaagt gtacatgtac
     8341 atccactaaa cctggagcaa ctacattacc actagcatct attatttttt catattcatc
     8401 actttcatca aacttgccta ttttataaat tttattttct tttataatta agtcaatatt
     8461 ctcatctctt tgacttttag ggtctattac tctaccattt ttaattaaaa tcatattatt
     8521 tctcctttat atttttaatt tatatagact catataatat tatacattat tttacatact
     8581 taaatacaaa attacgtttg acatcaatag cctttttagg acaaagttca tggcaacagt
     8641 aacatcttat acatttactt aagtctataa tagcaccctt agtccctatc tctatagtgg
     8701 atgcaggaca agcttccttg cattttccac attttatgca atcttgaaat cttaccacag
     8761 gctttggtgt tataagcttt gaaataggtt catttaaaaa ttttggtagc tttacggaaa
     8821 acaatcttaa atctttactt acatttggaa tcttgtaatt ttttataatt aaatctttta
     8881 tatcttcacc taaaatttca atatctgaaa aatcttcttg tatatatcct ctcttaatag
     8941 cacctctaat tgtagcaact ttatttggaa gtaaacttat aatcttacat gcaacaacat
     9001 ctatagcata tggattttgt gatgctaata aaactcctat ttctcgagta gtaccagctg
     9061 aaggtccatc accttccata cctactatac catccataat tgtcaatgtt ggttttatat
     9121 aatcacaaat atctaaaagt acattttcgc aaaacaactc ttctgtagga aatctataat
     9181 gtatctctgc ttttttcaat ccagctatgc atccatataa gttttttgta ccaccagtaa
     9241 aagttgccat tatatgagtc tttaatttac aaagatttat tatataatct gaatctacta
     9301 ttggtttaat aatatccata tattttagaa catgtgcttt ttcactatgt atcttaacat
     9361 ctgaaacatc ataatttaac ttaatgccca gttcatttgc tacttcttca attccagatg
     9421 ccttataaat gctcttaaga gaactttgat tatatggacc accagggcta tctgctattg
     9481 taatatcaca atttaactct aaaagttttt cacatacaac ttttacaacc ataggatgtg
     9541 tagttgtagc ttcttcaggc ttttttttca ttaataaatt tggttttata agtactttgc
     9601 ttcctttttt aacaaactta tctataccac caatatcctc taaatttttt tcaatagata
     9661 actttacttc ttcataattg taagttttac aactcctaac agataccgac ttcatattat
     9721 ctccatcctt ctatcacaac agtttattta tataatttaa aaaaggagaa accaaagttt
     9781 ctccttttaa tctatgtttt aattgtttta attattattt tccaaaatgt ctgtttaata
     9841 atcctaagaa tgctttacca tgtctagctt cgtctttaca catttcatgt actgtatcat
     9901 gtatagcatc taatcctaat tctttagctc tcttagctaa ttttaatttt ccatcagttg
     9961 caccatactc agcatcaact ctaactctta agttttcttt tgtatctgca actacaactt
    10021 ctccaagaag ttctgcaaat ttagcagcat gttcagcctc ttcaaaagct attctcttat
    10081 acgcttcagc tacttctgga taaccttctc tatcagcttg tctactcatt gctaaataca
    10141 ttcctacttc tgtacactca ccagtaaagt tagctcttaa tccttcgatt atttctgcat
    10201 ctacaccttg agctactcct attctatgtt catcagccca aaccatttcg cctttcattt
    10261 cttcaaattt atcagctcca actttacata ctggacattg tgctggtgca gcatctcctt
    10321 catgtatata tccacatact gtacaaacaa attttttcat aattaatttc ctcccaattt
    10381 tatataatta aatttttatt tttaagttta ataacttgct acaagaatat atatacccca
    10441 gactttatag cttaaacact aaaaataaaa tttatttcct aattaatttt atcactttga
    10501 aacaccagta tcaaccaagt tttgatgtca aatttctaca taatacataa tttatttgat
    10561 taattaggaa atagttgtca tattaaatac tttctaatga agactcctct gaatctttaa
    10621 gtttcacttt tttgctcttt aaaaaagata atttagtttc tgcagttatg attgcaacaa
    10681 atatcaagat actaccaata accaattttg ctgtaataag ctcctttaaa attattatag
    10741 aaaatattgt accaaataca gcctctgtgg ataatattat agctgttcta gttccatcaa
    10801 ctcttttttg acatatagtc tgaaataaaa atccaattgt agtactaaat acagctagat
    10861 acatagttcc catgtaacca gataattcag cttccatctt taattgacct gcaaatgttt
    10921 gaactacaac agacattaaa aacgctactg taaattgaat agctgtcaat gccattgggt
    10981 tgttatcttt tgcaaactca cttgtaaaga atatatgaaa tgcaaatccg aatgaacata
    11041 taagtgttag aaaatctcca aaattaatag agaaatcagc ttctaaagaa agtatcccaa
    11101 ttcctataag agctaccaaa ctacttatta ttcctatttt atcaagtttt cttctataaa
    11161 gtataaatcc tatgaatgga actattacaa cattagctgc tgttataaac gcatttttag
    11221 atggtgtagt gtacataagt ccaatagttt gtactgtgaa tgctagaaat aaaaatattc
    11281 ctaaaatccc tcctgctttt aataattttt tacccatatt ggcacgaatc tgtttaaaaa
    11341 acagtaaatt cataattata gctgccaaga aaaatcttaa agttatgatt tgtagaggtg
    11401 taagcccacc gtctaatgca aattgggttg ctacaaagcc acttccccat ataattgcta
    11461 taaatattag tcctatttct ccaaagtatt ttttcaataa agctccctcc tttttatttt
    11521 tttgcaaaat tataaacccc ctaaacagca agagagtttt tctataagca ttactataaa
    11581 ttaatatagt aaattttttc agtattttaa atctaaattt acatacattt tgagctcaaa
    11641 tttatatcca ctatttaaca gtaatatatt aatcattttt tgtaaacacc tttttaataa
    11701 atttttatat tttttatatt tttttaccta tactaaattt ttttatagat atttttaata
    11761 tatatgatac tttaatatta tgcaaataat gtcatataag ttacacttta gttatcttag
    11821 ttacaacttt gtcggtttac tttttctact ttcttcgata taattataat atatttttga
    11881 tacaattcga caaaaggggg aatgctgtaa ttatggtaaa aaaaagaaaa aagaaaactg
    11941 ttacacttac tatacttgtt gtaatagtta ttggtatttt ttctatggca tgggcggaca
    12001 tgtctagaaa aaaagaaatg aaagaaactc aagaaagact taaagctgaa agattagaaa
    12061 aagaaagaat tgaaaaagaa aaaaatgaac ctattattgg tgcaaaaaaa gaagctgtaa
    12121 aagaatatgg atatgacgca aaaatagttt gggacaaact taacaaatac gactattcaa
    12181 acaatggaga aaagatagtc tttcttacat ttgatgatgg accatctact actaatactc
    12241 cacaagttct agatatcttg aaaagacatg gtgttagagg aactttcttt attaagggtg
    12301 attctcttga aagaaaagga gctaatgaaa ttttaaaaag aacatttgac gaaggaaatg
    12361 caatagccca tcactcttat actcatgact ataaaaaact atatcctaac agaagcctaa
    12421 atctcgatac attcgtaaat gaacttaaca aaactgatga agctatgaaa aaagtcttag
    12481 gaaagaattt ttcctcaaat gtagttagat gcccaggcgg gtacatgtca tggaaaaata
    12541 tggaacctct aggaaactac ttaaaagaga aaaacatggc atcaattgat tggaacgctt
    12601 taaatgctga tgcagaaggt aaaaagaaaa atgcacaaga attatttgaa catgcaaaga
    12661 aatcttctga aggcaaagaa atggttgttc ttttaatgca tgatacttat ggaaaacaag
    12721 aaacagtaaa tgcattggac caaattataa catattttaa agacaatggt tatcaattta
    12781 aaattttaat ataataaaaa agcaagttta ttgaatgtac attttatact caatagactt
    12841 gcttttactt tactctattt tactagctcg atttttacaa attttttctt tcctatttga
    12901 acaactaatt cactctcttt tacaatagtg cttaaatcct ctactttttc accatttacc
    12961 tttactccac cttgagaagc taatcttcta acttcactct tactcttaac aatctcatta
    13021 gaaactagta cttcgattaa gtcaaatcca tcttccttaa cttgaattgt ttgtatatct
    13081 tcaggtattt gacctttttg gaatacagat ttaaatcttt cttcagcttc tttagcagat
    13141 tcttctccat ggtataatgt tactatttct cttgctaagt tcatctttat atctcttgga
    13201 tttactgaac cctctttcaa ctgagattct attttattaa cttcatcagg atgaacatct
    13261 gtcactaaat tgtagtattt aattataagt tcatcaggaa tttccataga tttttggtac
    13321 attattcccg cttcttcatc tattccaata tagttaccta aactcttact cattttttct
    13381 ttaccatcta atccttctat taaaggcatc attattacta tttgagactc cataccaaat
    13441 tctctttgta atgatcttcc cattagtaaa ttgaatcttt ggtcagttcc accaagttct
    13501 atatcagctt ctaatgctat agagtcatat gcttgcatta atgggtagaa gaactcatgt
    13561 actgatattg gcatttgtcc ttcatatctc tttttaaaat cttctctttc taacattctc
    13621 gcaacagtaa tagttgctgc taattttatt acatcttcaa aatttaactt agctaaccac
    13681 tcgctattaa atcttacaat agttttttct ttatctaaaa ctttgaaaat ttgttcttca
    13741 taagtttttg catttgcaag aacttgctct gttgttaaag cttttctagc tttagatttt
    13801 ccagttgggt ctccaatttt tcctgtaaaa tctccaatta ttattactat ttgatgacct
    13861 aaatcttgta attgtttcat ctttctaaga actactgtat gccctaaatg tatatctgga
    13921 gcacttgggt ctagacccaa ttttactatc ataggctttc cttctttttc tgatttaata
    13981 agcttttccc ttaactcttt ctcatctatt aaatcatcta cacctttcat aattattcgc
    14041 atttgctcgt ctatcgattt catttttcat tcctcctaaa atatttatat aatctataat
    14101 ttttattttt ataataaaaa aagctttcat cctcttaaaa ggacgaaagc attgcttacg
    14161 tgttaccacc ttagttcatc aatatctcac gatattaatc tcttagagta caattacata
    14221 tactcgtgta ctataacgtg tactaatccg atatctctta atggttgtta tctttcagag
    14281 atagtgctct gagatgtatt caaactaaat caatttattc catttcacca aactggaact
    14341 ctctgttaaa taaaatctag ccctacttct tctcttcata gcatttatat attcagatta
    14401 tttcatcaat tacaagaaat ttttcttaac ttactcataa tatattatta tttttatatt
    14461 ttgtcaacct tttttattat tatatacatg tttttttaca ttatactact tttttatatt
    14521 ttttaccaat acccaaaatt tatttttcaa tcaaaagatt tatttgttta ttgactatat
    14581 tactctgact caatcttata tttattttct tatttaaaaa tgcgtttata attattaaac
    14641 taataactgt tacatcaaat ataaaaaaca gcaataaaat tattatcaaa aataaaattc
    14701 tagcatataa taattttcta aacttagcta ttataaagtt ttgtagtgac atacatgagt
    14761 ctctaatgat aaaatagaca cttatatagg ttattgatat aaagattgtt tttaaataaa
    14821 cagaatcaac tttaacattc aacagtttta gaacttcaaa aatggctaga taagaaacac
    14881 atatgtaaaa agcttttttt gagcaacata aaaattggtt taaatttcta aagttcttaa
    14941 atattaaaaa tttttttaaa gaattagaat ctaaaatatg caatttattc cctaaaatta
    15001 aacttaataa atatatgcaa aacaccatac caaaaggaaa caatgctatc attaaataat
    15061 aaataaaatt tgtataccca ttaaaataaa tccatttgct atatccttga aattctttca
    15121 taaaactata acctattaat gttgaagcat atatatcaat gaaaaccatt acaataaccc
    15181 caaatactga accatatact aaatcatcca taaccattgt aggcttattt attagatagc
    15241 cacacattat accaagtaat acactctgac ttgcccaaat agcagttcct agatttccaa
    15301 gtaccagaga aattattaaa agcgaagtaa ttccagataa aatggtatac tttaaatcac
    15361 atttcaagca gattatacaa aaaaatactg gcactataaa atctaagtaa atagcatatc
    15421 caaatccagt actcacagct ataattgtta ctactatgaa taaagatgac aataaagctg
    15481 cttcagttaa ttttttagta gtattcaaaa cactgcctcc ataaatcata aattttctaa
    15541 ctaaagtttt gtttttaata tagtatatca ctttttaaaa atatttttaa taaatataaa
    15601 ttttaataaa ggtatttatg ttaaaaatgg tataatgtat cttaatattc agttttttaa
    15661 gatgctagga ggtaattact atgtttaaaa ttaaacactt taatgatttg agtttagatg
    15721 aattttatga aatagcaaaa tcaagatatg aggtatttgc ttgtgaacag aagatatttt
    15781 cattaaacga ttatgatgat atagataaaa gctcttatca catattttta aaagaaaatg
    15841 gattgatttg tgcttacgct agaataattc ctaaagaata cagctcttat aatgatgttt
    15901 ccattggtag agttttagtt ctttctagtc atagaagaaa aggattagca aaacaaatga
    15961 tggattgtgc tatagatttt ataaaagtta acttacacga aaataatata accttatcag
    16021 cacaaactta tataaagaac ctttacttat cttgtggttt taaagaaatc tcagaagtat
    16081 acgacgaagc aggtattgaa catattaaaa tgagattata gtgttgacaa gctaatattg
    16141 ttaatatata atttaagtat aaattaaata ttgaattgat atttaggtat ttgaaaatac
    16201 cttacataga gtcagaaggc ttttgctttc tgactttttt attatcaaaa ttcaataatt
    16261 atatggattc aaagtgttaa tttttattct atattctact ttccccaaaa gtaagaatta
    16321 tacccaactt tgtatgatag gcatcttgcc tatctttttt ttgtaaaagt aacaaggtaa
    16381 taatataatg catagtttaa aataagaaga ttagcccagg ctaaatttca agcatggagg
    16441 tgctattatg aaaaacctta acgatattca agtgaaaaaa actgtcaaag ttgaagatat
    16501 attatctagt ggtaatttaa gagaaagaat gttagcgctt ggtcttacta gaggagctgt
    16561 tatagatgta gttagaaaag gtccaaaaaa taatttaact gtctataaaa tcagaggtag
    16621 caagatagct ctacgacaag aagaaagtag cttaatatta gtttctgaca tttaaaaatt
    16681 tctagcagga ggatattaat atggggttga cacataactc aactaaaatg agttctttaa
    16741 aagacatgtt tgatatagac aataaggaag accagtttgt aattgcattg gctggcaatc
    16801 caaatacagg taaaagtact gtatttaatc atcttacagg acttagacag catactggaa
    16861 actggccagg aaaaactgta gctacagctc gtggtaattt taaatataaa aataccgaat
    16921 atgcattaat agatttacca ggaacatatt ctttatttgc cttatcacaa gaagaaattg
    16981 tagctcgtga ttttatatgt tttggtaatc cagatgcagt aatagtagta tgtgatgcta
    17041 catgtcttga aagaaattta aatttagtat ttcaagtgat ggaacttaca gataaagtta
    17101 ttttgtgtat taatttaata gatgaagcta gaaaaaaagg cattactatt gataaaaaac
    17161 ttttagagga ttctcttggt attccagtta ttttaactgc tgcaagaaat ggctctggta
    17221 tggatgaact attggatact ttaaatgatg tatcttttga caaatataaa ttaaataata
    17281 aacctgttag atacaatgaa aatatagaga atgtggtaaa atcaattcaa ccagaattgg
    17341 ataatattat accaggtata aattctagat ggttgggact tagacttatt gatggcgatg
    17401 aaagtatatt tgaatctatg agtaattata tagacaaaga ttctatagat gctattaatg
    17461 aagtaaagaa aaagattcca gacaatataa ataaacagaa aataagagat gagtttacta
    17521 aaatcaacta tgattatgct aaaaaactga gtgatgagtg ttgttctaat gttgctaaaa
    17581 aatcaactga tagagaagaa aaagtagata aaatattaac atctaaaata tttgggcttc
    17641 caataatgct cttattatta ggtactatat tgtggattac tatagaagga gctaactatc
    17701 catccacttt attatctaat ttattactcg gatttgagcc aagtatttcg ggtatattaa
    17761 atagtattaa ttgtccatca tggttaaatg atatgttggt tttaggatta tacagaacat
    17821 tagcatgggt aatctctgta atgctacctc ctatggcaat attttttcca ttatttaccc
    17881 tacttgagga ttttggatat ttgccaagag ttgcatttaa cttagaccac ctatttaaaa
    17941 aagcttgtgc acatggaaaa cagtgtttga ctatgtgtat gggatttgga tgtaatgcag
    18001 ctggagttat tggttgtagg ataattgact ctccaaggga aagacttata gctatactta
    18061 caaacaactt tgttccttgt aatggtcgtt ttcctacttt gatagctata tctacaatat
    18121 tttttagctc agttataacc aactctttcg tatcaagcgt agcaactgca ctttgcataa
    18181 ctttactgat aattctaggt gttataataa cattgttggt ttcttatact ctttctaaaa
    18241 cgctattaaa aggagttccg tctacattta cattagaact tcctccttat agagttcctc
    18301 aaataggaag aactttatat acctctataa tagatagaac aatatttgtt ttaggaaggg
    18361 ctgtaatggt agccatacct gctggtgtca taacttggat atttgcaaat atatatatag
    18421 gagatttaag tatattatca catgttgcaa actttttaga ccctttagct aaattaattg
    18481 gtcttgatgg ttttatatta cttgctttta ttcttggatt cccagcaaat gagatagttg
    18541 tacctatact tttaatggct tacctagcaa ctggttctat gatagaatta gatagcttta
    18601 gtgctttagg tcaagttctt agagaacatg gatggactta tttgacagct ttaaatgtta
    18661 tgctgttcag tttactgcat tggccttgtg caacaacact tttaacaata aaaaaagaaa
    18721 ctggtagttt aaaatggact gccttaggat tcttaatgcc tactatactt gcttttgttg
    18781 tttgtttttt aacaactaca gtttacaatt tatttatata attagtatta actgacagta
    18841 gacatatatt agagaaacca tttaaaaatt tatatacttt attataaagc atagagatat
    18901 attcaatgat aagtaaaatt acttaacatt tgaatatatc tctattttaa ttcttatctg
    18961 gatacttgga ttgaaatttc agtcatataa ttatcttcat tactttcaca aaaaaagtca
    19021 agtatactct cctcatacga atttccaaca attttatatt cattttcata tatatatttt
    19081 ttcaattttt cgtaagattt atatgaatcc tcatattttc caatatgttt tatgcaagca
    19141 tacattcctt ctggctttac atatagcttt ttatccttta tttttttatc tatcttcaaa
    19201 taaaaacttg atatacttaa atagt
//
LOCUS       DACULQ010000068        19030 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.068, whole genome shotgun sequence.
ACCESSION   DACULQ010000068 DACULQ010000000
VERSION     DACULQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.068"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..6842
                     /gene="tcdB"
                     /locus_tag="JA154_09455"
                     /gene_synonym="toxB"
     CDS             <1..6842
                     /gene="tcdB"
                     /locus_tag="JA154_09455"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HAT7996090.1"
                     /translation="NNNLTPVEKNLHFVWIGGQINDTAINYINQWKDVNSDYNVNVFY
                     DSNAFLINTLKKTVVESAINDTLESFRENLNDPRFDYNKFFRKRMEIIYDKQKNFINY
                     YKAQREENPELIIDDIVKTYLSNEYSKEIDELNTYIEESLNKITQNSGNDVRNFEEFK
                     NGESFNLYEQELVERWNLAAASDILRISALKEIGGMYLDVDMLPGIQPDLFESIEKPS
                     SVTVDFWEMTKLEAIMKYKEYIPEYTSEHFDMLDEEVQSSFESVLASKSDKSEIFSSL
                     GDMEASPLEVKIAFNSKGIINQGLISVKDSYCSNLIVKQIENRYKILNNSLNPAISED
                     NDFNTTTNTFIDSIMAEANADNGRFMMELGKYLRVGFFPDVKTTINLSGPEAYAAAYQ
                     DLLMFKEGSMNIHLIEADLRNFEISKTNISQSTEQEMASLWSFDDARAKAQFEEYKRN
                     YFEGSLGEDDNLDFSQNIVVDKEYLLEKISSLARSSERGYIHYIVQLQGDKISYEAAC
                     NLFAKTPYDSVLFQKNIEDSEIAYYYNPGDGEIQEIDKYKIPSIISDRPKIKLTFIGH
                     GKDEFNTDIFAGFDVDSLSTEIEAAIDLAKEDISPKSIEINLLGCNMFSYSINVEETY
                     PGKLLLKVKDKISELMPSISQDSIIVSANQYEVRINSEGRRELLDHSGEWINKEESII
                     KDISSKEYISFNPKENKITVKSKNLPELSTLLQEIRNNSNSSDIELEEKVMLTECEIN
                     VISNIDTQIVEERIEEAKNLTSDSINYIKDEFKLIESISDALCDLKQQNELEDSHFIS
                     FEDISETDEGFSIRFINKETGESIFVETEKTIFSEYANHITEEISKIKGTIFDTVNGK
                     LVKKVNLDTTHEVNTLNAAFFIQSLIEYNSSKESLSNLSVAMKVQVYAQLFSTGLNTI
                     TDAAKVVELVSTALDETIDLLPTLSEGLPIIATIIDGVSLGAAIKELSETSDPLLRQE
                     IEAKIGIMAVNLTTATTAIITSSLGVASGFSILLVPLAGISAGIPSLVNNELVLRDKA
                     TKVVDYFKHVSLVETEGVFTLLDDKIMMPQDDLVISEIDFNNNSIVLGKCEIWRMEGG
                     SGHTVTDDIDHFFSAPSITYREPHLSIYDVLEVQKEELDLSKDLMVLPNAPNRVFAWE
                     TGWTPGLRSLENDGTKLLDRIRDNYEGEFYWRYFAFIADALITTLKPRYEDTNIRINL
                     DSNTRSFIVPIITTEYIREKLSYSFYGSGGTYALSLSQYNMGINIELSESDVWIIDVD
                     NVVRDVTIESDKIKKGDLIEGILSTLSIEENKIILNSHEINFSGEVNGSNGFVSLTFS
                     ILEGINAIIEVDLLSKSYKLLISGELKILMLNSNHIQQKIDYIGFNSELQKNIPYSFV
                     DSEGKENGFINGSTKEGLFVSELPDVVLISKVYMDDSKPSFGYYSNNLKDVKVITKDN
                     VNILTGYYLKDDIKISLSLTLQDEKTIKLNSVHLDESGVAEILKFMNRKGNTNTSDSL
                     MSFLESMNIKSIFVNFLQSNIKFILDANFIISGTTSIGQFEFICDENDNIQPYFIKFN
                     TLETNYTLYVGNRQNMIVEPNYDLDDSGDISSTVINFSQKYLYGIDSCVNKVVISPNI
                     YTDEINITPVYETNNTYPEVIVLDANYINEKINVNINDLSIRYVWSNDGNDFILMSTS
                     EENKVSQVKIRFVNVFKDKTLANKLSFNFSDKQDVPVSEIILSFTPSYYEDGLIGYDL
                     GLVSLYNEKFYINNFGMMVSGLIYINDSLYYFKPPVNNLITGFVTVGDDKYYFNPING
                     GAASIGETIIDDKNYYFNQSGVLQTGVFSTEDGFKYFAPANTLDENLEGEAIDFTGKL
                     IIDENIYYFDDNYRGAVEWKELDGEMHYFSPETGKAFKGLNQIGDYKYYFNSDGVMQK
                     GFVSINDNKHYFDDSGVMKVGYTEIDGKHFYFAENGEMQIGVFNTEDGFKYFAHHNED
                     LGNEEGEEISYSGILNFNNKIYYFDDSFTAVVGWKDLEDGSKYYFDEDTAEAYIGLSL
                     INDGQYYFNDDGIMQVGFVTINDKVFYFSDSGIIESGVQNIDDNYFYIDDNGIVQIGV
                     FDTSDGYKYFAPANTVNDNIYGQAVEYSGLVRVGEDVYYFGETYTIETGWIYDMENES
                     DKYYFNPETKKACKGINLIDDIKYYFDEKGIMRTGLISFENNNYYFNENGEMQFGYIN
                     IEDKMFYFGEDGVMQIGVFNTPDGFKYFAHQNTLDENFEGESINYTGWLDLDEKRYYF
                     TDEYIAATGSVIIDGEEYYFDPDTAQLVISE"
     gene            6964..7464
                     /gene="tcdE"
                     /locus_tag="JA154_09460"
     CDS             6964..7464
                     /gene="tcdE"
                     /locus_tag="JA154_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HAT7996091.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            7573..7704
                     /locus_tag="JA154_09465"
     CDS             7573..7704
                     /locus_tag="JA154_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996092.1"
                     /translation="MPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKDGAWSVGKIY"
     gene            8192..16324
                     /gene="tcdA"
                     /locus_tag="JA154_09470"
                     /gene_synonym="toxA"
     CDS             8192..16324
                     /gene="tcdA"
                     /locus_tag="JA154_09470"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HAT7996093.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(16659..17357)
                     /gene="tcdC"
                     /locus_tag="JA154_09475"
     CDS             complement(16659..17357)
                     /gene="tcdC"
                     /locus_tag="JA154_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HAT7996094.1"
                     /translation="MFSKKNDGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            17783..18028
                     /locus_tag="JA154_09480"
     CDS             17783..18028
                     /locus_tag="JA154_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HAT7996095.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLI
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(18246..18515)
                     /locus_tag="JA154_09485"
     CDS             complement(18246..18515)
                     /locus_tag="JA154_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HAT7996096.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(18688..18926)
                     /locus_tag="JA154_09490"
                     /pseudo
     CDS             complement(18688..18926)
                     /locus_tag="JA154_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7493 a         2194 c         2904 g         6439 t
ORIGIN      
        1 agaataataa tttaactcca gttgagaaaa atttacattt tgtttggatt ggaggtcaaa
       61 taaatgacac tgctattaat tatataaatc aatggaaaga tgtaaatagt gattataatg
      121 ttaatgtttt ttatgatagt aatgcatttt tgataaacac attgaaaaaa actgtagtag
      181 aatcagcaat aaatgataca cttgaatcat ttagagaaaa cttaaatgac cctagatttg
      241 actataataa attcttcaga aaacgtatgg aaataattta tgataaacag aaaaatttca
      301 taaactacta taaagctcaa agagaagaaa atcctgaact tataattgat gatattgtaa
      361 agacatatct ttcaaatgag tattcaaagg agatagatga acttaatacc tatattgaag
      421 aatccttaaa taaaattaca cagaatagtg gaaatgatgt tagaaacttt gaagaattta
      481 aaaatggaga gtcattcaac ttatatgaac aagagttggt agaaaggtgg aatttagctg
      541 ctgcttctga catattaaga atatctgcat taaaagaaat tggtggtatg tatttagatg
      601 ttgatatgtt accaggaata caaccagact tatttgagtc tatagagaaa cctagttcag
      661 taacagtgga tttttgggaa atgacaaagt tagaagctat aatgaaatac aaagaatata
      721 taccagaata tacctcagaa cattttgaca tgttagacga agaagttcaa agtagttttg
      781 aatctgttct agcttctaag tcagataaat cagaaatatt ctcatcactt ggtgatatgg
      841 aggcatcacc actagaagtt aaaattgcat ttaatagtaa gggtattata aatcaagggc
      901 taatttctgt gaaagactca tattgtagca atttaatagt aaaacaaatc gagaatagat
      961 ataaaatatt gaataatagt ttaaatccag ctattagcga ggataatgat tttaatacta
     1021 caacgaatac ctttattgat agtataatgg ctgaagctaa tgcagataat ggtagattta
     1081 tgatggaact aggaaagtat ttaagagttg gtttcttccc agatgttaaa actactatta
     1141 acttaagtgg ccctgaagca tatgcggcag cttatcaaga tttattaatg tttaaagaag
     1201 gcagtatgaa tatccatttg atagaagctg atttaagaaa ctttgaaatc tctaaaacta
     1261 atatttctca atcaactgaa caagaaatgg ctagcttatg gtcatttgac gatgcaagag
     1321 ctaaagctca atttgaagaa tataaaagga attattttga aggttctctt ggtgaagatg
     1381 ataatcttga tttttctcaa aatatagtag ttgacaagga gtatctttta gaaaaaatat
     1441 cttcattagc aagaagttca gagagaggat atatacacta tattgttcag ttacaaggag
     1501 ataaaattag ttatgaagca gcatgtaact tatttgcaaa gactccttat gatagtgtac
     1561 tatttcagaa aaatatagaa gattcagaaa ttgcatatta ttataatcct ggagatggtg
     1621 aaatacaaga aatagacaag tataaaattc caagtataat ttctgataga cctaagatta
     1681 aattaacatt tattggtcat ggtaaagatg aatttaatac tgatatattt gcaggttttg
     1741 atgtagattc attatccaca gaaatagaag cagcaataga tttagctaaa gaggatattt
     1801 ctcctaagtc aatagaaata aatttattag gatgtaatat gtttagctac tctatcaacg
     1861 tagaggagac ttatcctgga aaattattac ttaaagttaa agataaaata tcagaattaa
     1921 tgccatctat aagtcaagac tctattatag taagtgcaaa tcaatatgaa gttagaataa
     1981 atagtgaagg aagaagagaa ttattggatc attctggtga atggataaat aaagaagaaa
     2041 gtattataaa ggatatttca tcaaaagaat atatatcatt taatcctaaa gaaaataaaa
     2101 ttacagtaaa atctaaaaat ttacctgagc tatctacatt attacaagaa attagaaata
     2161 attctaattc aagtgatatt gaactagaag aaaaagtaat gttaacagaa tgtgagataa
     2221 atgttatttc aaatatagat acgcaaattg ttgaggaaag gattgaagaa gctaagaatt
     2281 taacttctga ctctattaat tatataaaag atgaatttaa actaatagaa tctatttctg
     2341 atgcactatg tgacttaaaa caacagaatg aattagaaga ttctcatttt atatcttttg
     2401 aggacatatc agagactgat gagggattta gtataagatt tattaataaa gaaactggag
     2461 aatctatatt tgtagaaact gaaaaaacaa tattctctga atatgctaat catataactg
     2521 aagagatttc taagataaaa ggtactatat ttgatactgt aaatggtaag ttagtaaaaa
     2581 aagtaaattt agatactaca cacgaagtaa atactttaaa tgctgcattt tttatacaat
     2641 cattaataga atataatagt tctaaagaat ctcttagtaa tttaagtgta gcaatgaaag
     2701 tccaagttta cgctcaatta tttagtactg gtttaaatac tattacagat gcagccaaag
     2761 ttgttgaatt agtatcaact gcattagatg aaactataga cttacttcct acattatctg
     2821 aaggattacc tataattgca actattatag atggtgtaag tttaggtgca gcaatcaaag
     2881 agctaagtga aacgagtgac ccattattaa gacaagaaat agaagctaag ataggtataa
     2941 tggcagtaaa tttaacaaca gctacaactg caatcattac ttcatctttg ggggtagcta
     3001 gtggatttag tatactttta gttcctttag caggaatttc agcaggtata ccaagcttag
     3061 taaacaatga acttgtactt cgagataagg caacaaaggt tgtagattat tttaaacatg
     3121 tttcattagt tgaaactgaa ggagtattta ctttattaga tgataaaata atgatgccac
     3181 aagatgattt agtgatatca gaaatagatt ttaataataa ttcaatagtt ttaggtaaat
     3241 gtgaaatctg gagaatggaa ggtggttcag gtcatactgt aactgatgat atagatcact
     3301 tcttttcagc accatcaata acatatagag agccacactt atctatatat gacgtattgg
     3361 aagtacaaaa agaagaactt gatttgtcaa aagatttaat ggtattacct aatgctccaa
     3421 atagagtatt tgcttgggaa acaggatgga caccaggttt aagaagctta gaaaatgatg
     3481 gcacaaaact gttagaccgt ataagagata actatgaagg tgagttttat tggagatatt
     3541 ttgcttttat agctgatgct ttaataacaa cattaaaacc aagatatgaa gatactaata
     3601 taagaataaa tttagatagt aatactagaa gttttatagt tccaataata actacagaat
     3661 atataagaga aaaattatca tattctttct atggttcagg aggaacttat gcattgtctc
     3721 tttctcaata taatatgggt ataaatatag aattaagtga aagtgatgtt tggattatag
     3781 atgttgataa tgttgtgaga gatgtaacta tagaatctga taaaattaaa aaaggtgatt
     3841 taatagaagg tattttatct acactaagta ttgaagagaa taaaattatc ttaaatagcc
     3901 atgagattaa tttttctggt gaggtaaatg gaagtaatgg atttgtttct ttaacatttt
     3961 caattttaga aggaataaat gcaattatag aagttgattt attatctaaa tcatataaat
     4021 tacttatttc tggcgaatta aaaatattga tgttaaattc aaatcatatt caacagaaaa
     4081 tagattatat aggattcaat agcgaattac agaaaaatat accatatagc tttgtagata
     4141 gtgaaggaaa agagaatggt tttattaatg gttcaacaaa agaaggttta tttgtatctg
     4201 aattacctga tgtagttctt ataagtaagg tttatatgga tgatagtaag ccttcatttg
     4261 gatattatag taataatttg aaagatgtca aagttataac taaagataat gttaatatat
     4321 taacaggtta ttatcttaag gatgatataa aaatctctct ttctttgact ctacaagatg
     4381 aaaaaactat aaagttaaat agtgtgcatt tagatgaaag tggagtagct gagattttga
     4441 agttcatgaa tagaaaaggt aatacaaata cttcagattc tttaatgagc tttttagaaa
     4501 gtatgaatat aaaaagtatt ttcgttaatt tcttacaatc taatattaag tttatattag
     4561 atgctaattt tataataagt ggtactactt ctattggcca atttgagttt atttgtgatg
     4621 aaaatgataa tatacaacca tatttcatta agtttaatac actagaaact aattatactt
     4681 tatatgtagg aaatagacaa aatatgatag tggaaccaaa ttatgattta gatgattctg
     4741 gagatatatc ttcaactgtt atcaatttct ctcaaaagta tctttatgga atagacagtt
     4801 gtgttaataa agttgtaatt tcaccaaata tttatacaga tgaaataaat ataacgcctg
     4861 tatatgaaac aaataatact tatccagaag ttattgtatt agatgcaaat tatataaatg
     4921 aaaaaataaa tgttaatatc aatgatctat ctatacgata tgtatggagt aatgatggta
     4981 atgattttat tcttatgtca actagtgaag aaaataaggt gtcacaagtt aaaataagat
     5041 tcgttaatgt ttttaaagat aagactttgg caaataagct atcttttaac tttagtgata
     5101 aacaagatgt acctgtaagt gaaataatct tatcatttac accttcatat tatgaggatg
     5161 gattgattgg ctatgatttg ggtctagttt ctttatataa tgagaaattt tatattaata
     5221 actttggaat gatggtatct ggattaatat atattaatga ttcattatat tattttaaac
     5281 caccagtaaa taatttgata actggatttg tgactgtagg cgatgataaa tactacttta
     5341 atccaattaa tggtggagct gcttcaattg gagagacaat aattgatgac aaaaattatt
     5401 atttcaacca aagtggagtg ttacaaacag gtgtatttag tacagaagat ggatttaaat
     5461 attttgcccc agctaataca cttgatgaaa acctagaagg agaagcaatt gattttactg
     5521 gaaaattaat tattgacgaa aatatttatt attttgatga taattataga ggagctgtag
     5581 aatggaaaga attagatggt gaaatgcact attttagccc agaaacaggt aaagctttta
     5641 aaggtctaaa tcaaataggt gattataaat actatttcaa ttctgatgga gttatgcaaa
     5701 aaggatttgt tagtataaat gataataaac actattttga tgattctggt gttatgaaag
     5761 taggttacac tgaaatagat ggcaagcatt tctactttgc tgaaaacgga gaaatgcaaa
     5821 taggagtatt taatacagaa gatggattta aatattttgc tcatcataat gaagatttag
     5881 gaaatgaaga aggtgaagaa atctcatatt ctggtatatt aaatttcaat aataaaattt
     5941 actattttga tgattcattt acagctgtag ttggatggaa agatttagag gatggttcaa
     6001 agtattattt tgatgaagat acagcagaag catatatagg tttgtcatta ataaatgatg
     6061 gtcaatatta ttttaatgat gatggaatta tgcaagttgg atttgtcact ataaatgata
     6121 aagtcttcta cttctctgac tctggaatta tagaatctgg agtacaaaac atagatgaca
     6181 attatttcta tatagatgat aatggtatag ttcaaattgg tgtatttgat acttcagatg
     6241 gatataaata ttttgcacct gctaatactg taaatgataa tatttacgga caagcagttg
     6301 aatatagtgg tttagttaga gttggggaag atgtatatta ttttggagaa acatatacaa
     6361 ttgagactgg atggatatat gatatggaaa atgaaagtga taaatattat ttcaatccag
     6421 aaactaaaaa agcatgcaaa ggtattaatt taattgatga tataaaatat tattttgatg
     6481 agaagggcat aatgagaacg ggtcttatat catttgaaaa taataattat tactttaatg
     6541 agaatggtga aatgcaattt ggttatataa atatagaaga taagatgttc tattttggtg
     6601 aagatggtgt catgcagatt ggagtattta atacaccaga tggatttaaa tactttgcac
     6661 atcaaaatac tttggatgag aattttgagg gagaatcaat aaactatact ggttggttag
     6721 atttagatga aaagagatat tattttacag atgaatatat tgcagcaact ggttcagtta
     6781 ttattgatgg tgaggagtat tattttgatc ctgatacagc tcaattagtg attagtgaat
     6841 agataaaaat atgttaaata tatcctctta tacttaaata tataaaaata aacaaaatga
     6901 tacactacat aaagtgttct atctaatatg aagatttacc aataaaaagg tggactatga
     6961 tgaatgcaca gtagttcacc tttttatatt tctaatggta acaaaatatt tttttatata
     7021 aacctaggag gcgttatgaa tatgacaata tcttttttat cagagcatat atttataaag
     7081 ttagtaattt taactatatc atttgataca ttattaggat gtttaagtgc aataaaaagt
     7141 cgtaaattta attctagttt tggaatagat ggaggaatca gaaaagtagc aatgatagca
     7201 tgtatatttt ttttatcagt agttgacatt cttacaaagt ttaacttttt atttatgtta
     7261 ccacaagatt gtatcaattt tttaagacta aaacatcttg gaatatctga atttttctct
     7321 attttattta ttttatatga aagtgtaagt atattaaaaa atatgtgctt atgtggatta
     7381 ccagtaccta agagattaaa ggaaaaaata gcaattttac tagatgcaat gacagatgaa
     7441 atgaatgcta aggatgaaaa gtaagtaatg gtagatataa taaagatatt aacaaataaa
     7501 aagtgttatc caaataagaa tagctgaaag ttatcataat tcatgaaact aataatgaaa
     7561 acgagggagc agatgccaag agacacacaa gtattaaata catataattt cgaagcaagt
     7621 gttcattact atatagatga caaggtagta tatcaaacat tggttcacaa agatggtgca
     7681 tggtcagttg gtaaaatcta ttaagctaca ttagttacag atatcacaaa ctataatagt
     7741 taaacataga aatatgtgta aattgtgatg gaaattattc aaaaacacaa aaatacgtga
     7801 tgaaggacaa aatgatatag aaaataagta tcaaacctta ataaatgatt taattgatag
     7861 tttaaaagtt ataggaaaaa tatataaaga aataaaaaca ttaaaaaaat ataagatatg
     7921 tttacaaatt actatcagac aatctcctta tctaatagaa gagtcaatta actaattgag
     7981 tatctttaaa ttgaaatgtt aggaagtgat ttaaatatga aaacttaaat tataaaaaat
     8041 caatattaat ttatttttaa aaaatagaaa ggagtgtata agatttattt tcaaagttta
     8101 aaaacaagaa aatcaattta aatttcagaa ggaataaatg tggttataga agtggattta
     8161 ttatcaaaaa taataatact aggaggtttt tatgtcttta atatctaaag aagagttaat
     8221 aaaactcgca tatagcatta gaccaagaga aaatgagtat aaaactatac taactaattt
     8281 agacgaatat aataagttaa ctacaaacaa taatgaaaat aaatatttac aattaaaaaa
     8341 actaaatgaa tcaattgatg tttttatgaa taaatataaa acttcaagca gaaatagagc
     8401 actctctaat ctaaaaaaag atatattaaa agaagtaatt cttattaaaa attccaatac
     8461 aagccctgta gaaaaaaatt tacattttgt atggataggt ggagaagtca gtgatattgc
     8521 tcttgaatac ataaaacaat gggctgatat taatgcagaa tataatatta aactgtggta
     8581 tgatagtgaa gcattcttag taaatacact aaaaaaggct atagttgaat cttctaccac
     8641 tgaagcatta cagctactag aggaagagat tcaaaatcct caatttgata atatgaaatt
     8701 ttacaaaaaa aggatggaat ttatatatga tagacaaaaa aggtttataa attattataa
     8761 atctcaaatc aataaaccta cagtacctac aatagatgat attataaagt ctcatctagt
     8821 atctgaatat aatagagatg aaactgtatt agaatcatat agaacaaatt ctttgagaaa
     8881 aataaatagt aatcatggta tagatatcag ggctaatagt ttgtttacag aacaagagtt
     8941 attaaatatt tatagtcagg agttgttaaa tcgtggaaat ttagctgcag catctgacat
     9001 agtaagatta ttagccctaa aaaattttgg cggagtatat ttagatgttg atatgcttcc
     9061 aggtattcac tctgatttat ttaaaacaat atctagacct agctctattg gactagaccg
     9121 ttgggaaatg ataaaattag aggctattat gaagtataaa aaatatataa ataattatac
     9181 atcagaaaac tttgataaac ttgatcaaca attaaaagat aattttaaac tcattataga
     9241 aagtaaaagt gaaaaatctg agatattttc taaattagaa aatttaaatg tatctgatct
     9301 tgaaattaaa atagctttcg ctttaggcag tgttataaat caagccttga tatcaaaaca
     9361 aggttcatat cttactaacc tagtaataga acaagtaaaa aatagatatc aatttttaaa
     9421 ccaacacctt aacccagcca tagagtctga taataacttc acagatacta ctaaaatttt
     9481 tcatgattca ttatttaatt cagctaccgc agaaaactct atgtttttaa caaaaatagc
     9541 accatactta caagtaggtt ttatgccaga agctcgctcc acaataagtt taagtggtcc
     9601 aggagcttat gcgtcagctt actatgattt cataaattta caagaaaata ctatagaaaa
     9661 aactttaaaa gcatcagatt taatagaatt taaattccca gaaaataatc tatctcaatt
     9721 gacagaacaa gaaataaata gtctatggag ctttgatcaa gcaagtgcaa aatatcaatt
     9781 tgagaaatat gtaagagatt atactggtgg atctctttct gaagacaatg gggtagactt
     9841 taataaaaat actgccctcg acaaaaacta tttattaaat aataaaattc catcaaacaa
     9901 tgtagaagaa gctggaagta aaaattatgt tcattatatc atacagttac aaggagatga
     9961 tataagttat gaagcaacat gcaatttatt ttctaaaaat cctaaaaata gtattattat
    10021 acaacgaaat atgaatgaaa gtgcaaaaag ctacttttta agtgatgatg gagaatctat
    10081 tttagaatta aataaatata ggatacctga aagattaaaa aataaggaaa aagtaaaagt
    10141 aacctttatt ggacatggta aagatgaatt caacacaagc gaatttgcta gattaagtgt
    10201 agattcactt tccaatgaga taagttcatt tttagatacc ataaaattag atatatcacc
    10261 taaaaatgta gaagtaaact tacttggatg taatatgttt agttatgatt ttaatgttga
    10321 agaaacttat cctgggaagt tgctattaag tattatggac aaaattactt ccactttacc
    10381 tgatgtaaat aaaaattcta ttactatagg agcaaatcaa tatgaagtaa gaattaatag
    10441 tgagggaaga aaagaacttc tggctcactc aggtaaatgg ataaataaag aagaagctat
    10501 tatgagcgat ttatctagta aagaatacat tttttttgat tctatagata ataagctaaa
    10561 agcaaagtcc aagaatattc caggattagc atcaatatca gaagatataa aaacattatt
    10621 acttgatgca agtgttagtc ctgatacaaa atttatttta aataatctta agcttaatat
    10681 tgaatcttct attggtgatt acatttatta tgaaaaatta gagcctgtta aaaatataat
    10741 tcacaattct atagatgatt taatagatga gttcaatcta cttgaaaatg tatctgatga
    10801 attatatgaa ttaaaaaaat taaataatct agatgagaag tatttaatat cttttgaaga
    10861 tatctcaaaa aataattcaa cttactctgt aagatttatt aacaaaagta atggtgagtc
    10921 agtttatgta gaaacagaaa aagaaatttt ttcaaaatat agcgaacata ttacaaaaga
    10981 aataagtact ataaagaata gtataattac agatgttaat ggtaatttat tggataatat
    11041 acagttagat catacttctc aagttaatac attaaacgca gcattcttta ttcaatcatt
    11101 aatagattat agtagcaata aagatgtact gaatgattta agtacctcag ttaaggttca
    11161 actttatgct caactattta gtacaggttt aaatactata tatgactcta tccaattagt
    11221 aaatttaata tcaaatgcag taaatgatac tataaatgta ctacctacaa taacagaggg
    11281 gatacctatt gtatctacta tattagacgg aataaactta ggtgcagcaa ttaaggaatt
    11341 actagacgaa catgacccat tactaaaaaa agaattagaa gctaaggtgg gtgttttagc
    11401 aataaatatg tcattatcta tagctgcaac tgtagcttca attgttggaa taggtgctga
    11461 agttactatt ttcttattac ctatagctgg tatatctgca ggaatacctt cattagttaa
    11521 taatgaatta atattgcatg ataaggcaac ttcagtggta aactatttta atcatttgtc
    11581 tgaatctaaa aaatatggcc ctcttaaaac agaagatgat aaaattttag ttcctattga
    11641 tgatttagta atatcagaaa tagattttaa taataattcg ataaaactag gaacatgtaa
    11701 tatattagca atggaggggg gatcaggaca cacagtgact ggtaatatag atcacttttt
    11761 ctcatctcca tctataagtt ctcatattcc ttcattatca atttattctg caataggtat
    11821 agaaacagaa aatctagatt tttcaaaaaa aataatgatg ttacctaatg ctccttcaag
    11881 agtgttttgg tgggaaactg gagcagttcc aggtttaaga tcattggaaa atgacggaac
    11941 tagattactt gattcaataa gagatttata cccaggtaaa ttttactgga gattctatgc
    12001 ttttttcgat tatgcaataa ctacattaaa accagtttat gaagacacta atattaaaat
    12061 taaactagat aaagatacta gaaacttcat aatgccaact ataactacta acgaaattag
    12121 aaacaaatta tcttattcat ttgatggagc aggaggaact tactctttat tattatcttc
    12181 atatccaata tcaacgaata taaatttatc taaagatgat ttatggatat ttaatattga
    12241 taatgaagta agagaaatat ctatagaaaa tggtactatt aaaaaaggaa agttaataaa
    12301 agatgtttta agtaaaattg atataaataa aaataaactt attataggca atcaaacaat
    12361 agatttttca ggcgatatag ataataaaga tagatatata ttcttgactt gtgagttaga
    12421 tgataaaatt agtttaataa tagaaataaa tcttgttgca aaatcttata gtttgttatt
    12481 gtctggggat aaaaattatt tgatatccaa tttatctaat actattgaga aaatcaatac
    12541 tttaggccta gatagtaaaa atatagcgta caattacact gatgaatcta ataataaata
    12601 ttttggagct atatctaaaa caagtcaaaa aagcataata cattataaaa aagacagtaa
    12661 aaatatatta gaattttata atgacagtac attagaattt aacagtaaag attttattgc
    12721 tgaagatata aatgtattta tgaaagatga tattaatact ataacaggaa aatactatgt
    12781 tgataataat actgataaaa gtatagattt ctctatttct ttagttagta aaaatcaagt
    12841 aaaagtaaat ggattatatt taaatgaatc cgtatactca tcttaccttg attttgtgaa
    12901 aaattcagat ggacaccata atacttctaa ttttatgaat ttatttttgg acaatataag
    12961 tttctggaaa ttgtttgggt ttgaaaatat aaattttgta atcgataaat actttaccct
    13021 tgttggtaaa actaatcttg gatatgtaga atttatttgt gacaataata aaaatataga
    13081 tatatatttt ggtgaatgga aaacatcgtc atctaaaagc actatattta gcggaaatgg
    13141 tagaaatgtt gtagtagagc ctatatataa tcctgatacg ggtgaagata tatctacttc
    13201 actagatttt tcctatgaac ctctctatgg aatagataga tatatcaata aagtattgat
    13261 agcacctgat ttatatacaa gtttaataaa tattaatacc aattattatt caaatgagta
    13321 ctaccctgag attatagttc ttaacccaaa tacattccac aaaaaagtaa atataaattt
    13381 agatagttct tcttttgagt ataaatggtc tacagaagga agtgacttta ttttagttag
    13441 atacttagaa gaaagtaata aaaaaatatt acaaaaaata agaatcaaag gtatcttatc
    13501 taatactcaa tcatttaata aaatgagtat agattttaaa gatattaaaa aactatcatt
    13561 aggatatata atgagtaatt ttaaatcatt taattctgaa aatgaattag atagagatca
    13621 tttaggattt aaaataatag ataataaaac ttattactat gatgaagata gtaaattagt
    13681 taaaggatta atcaatataa ataattcatt attctatttt gatcctatag aatttaactt
    13741 agtaactgga tggcaaacta tcaatggtaa aaaatattat tttgatataa atactggagc
    13801 agctttaact agttataaaa ttattaatgg taaacacttt tattttaata atgatggtgt
    13861 gatgcagttg ggagtattta aaggacctga tggatttgaa tattttgcac ctgccaatac
    13921 tcaaaataat aacatagaag gtcaggctat agtttatcaa agtaaattct taactttgaa
    13981 tggcaaaaaa tattattttg ataatgactc aaaagcagtc actggatgga gaattattaa
    14041 caatgagaaa tattacttta atcctaataa tgctattgct gcagtcggat tgcaagtaat
    14101 tgacaataat aagtattatt tcaatcctga cactgctatc atctcaaaag gttggcagac
    14161 tgttaatggt agtagatact actttgatac tgataccgct attgccttta atggttataa
    14221 aactattgat ggtaaacact tttattttga tagtgattgt gtagtgaaaa taggtgtgtt
    14281 tagtacctct aatggatttg aatattttgc acctgctaat acttataata ataacataga
    14341 aggtcaggct atagtttatc aaagtaaatt cttaactttg aatggtaaaa aatattactt
    14401 tgataataac tcaaaagcag ttaccggatg gcaaactatt gatagtaaaa aatattactt
    14461 taatactaac actgctgaag cagctactgg atggcaaact attgatggta aaaaatatta
    14521 ctttaatact aacactgctg aagcagctac tggatggcaa actattgatg gtaaaaaata
    14581 ttactttaat actaacactg ctatagcttc aactggttat acaattatta atggtaaaca
    14641 tttttatttt aatactgatg gtattatgca gataggagtg tttaaaggac ctaatggatt
    14701 tgaatatttt gcacctgcta atacggatgc taacaacata gaaggtcaag ctatacttta
    14761 ccaaaatgaa ttcttaactt tgaatggtaa aaaatattac tttggtagtg actcaaaagc
    14821 agttactgga tggagaatta ttaacaataa gaaatattac tttaatccta ataatgctat
    14881 tgctgcaatt catctatgca ctataaataa tgacaagtat tactttagtt atgatggaat
    14941 tcttcaaaat ggatatatta ctattgaaag aaataatttc tattttgatg ctaataatga
    15001 atctaaaatg gtaacaggag tatttaaagg acctaatgga tttgagtatt ttgcacctgc
    15061 taatactcac aataataaca tagaaggtca ggctatagtt taccagaaca aattcttaac
    15121 tttgaatggc aaaaaatatt attttgataa tgactcaaaa gcagttactg gatggcaaac
    15181 cattgatggt aaaaaatatt actttaatct taacactgct gaagcagcta ctggatggca
    15241 aactattgat ggtaaaaaat attactttaa tcttaacact gctgaagcag ctactggatg
    15301 gcaaactatt gatggtaaaa aatattactt taatactaac actttcatag cctcaactgg
    15361 ttatacaagt attaatggta aacattttta ttttaatact gatggtatta tgcagatagg
    15421 agtgtttaaa ggacctaatg gatttgaata ctttgcacct gctaatactc ataataataa
    15481 catagaaggt caagctatac tttaccaaaa taaattctta actttgaatg gtaaaaaata
    15541 ttactttggt agtgactcaa aagcagttac cggattgcga actattgatg gtaaaaaata
    15601 ttactttaat actaacactg ctgttgcagt tactggatgg caaactatta atggtaaaaa
    15661 atactacttt aatactaaca cttctatagc ttcaactggt tatacaatta ttagtggtaa
    15721 acatttttat tttaatactg atggtattat gcagatagga gtgtttaaag gacctgatgg
    15781 atttgaatac tttgcacctg ctaatacaga tgctaacaat atagaaggtc aagctatacg
    15841 ttatcaaaat agattcctat atttacatga caatatatat tattttggta ataattcaaa
    15901 agcggctact ggttgggtaa ctattgatgg taatagatat tacttcgagc ctaatacagc
    15961 tatgggtgcg aatggttata aaactattga taataaaaat ttttacttta gaaatggttt
    16021 acctcagata ggggtgttta aagggtctaa tggatttgaa tactttgcac ctgctaatac
    16081 ggatgctaac aatatagaag gtcaagctat acgttatcaa aatagattcc tacatttact
    16141 tggaaaaata tattactttg gtaataattc aaaagcagtt actggatggc aaactattaa
    16201 tggtaaagta tattacttta tgcctgatac tgctatggct gcagctggtg gactttttga
    16261 gattgatggt gttatatatt tctttggtgt tgatggagta aaagcccctg ggatatatgg
    16321 ctaaaatata tgtttgataa aaaattattc ctgtgctact aagaaattat ttttatataa
    16381 taaatattga gatttaatta aaagtcatgt gttattgtaa tacatgactt ttagttaaaa
    16441 tttttctatc atttaataat ctattattct tgactatttt ataataaaat tcatatatgg
    16501 aaatattaat actaaataat taatagttga taaaaaatag ataatatgct aaaagcaaaa
    16561 actaatttag agccttgtaa ctgtttattt gcaattataa aaacatcttt aaacatattg
    16621 actataatat aaaatattaa ctataataca aaacaatatt aattaatttt ctctacagct
    16681 atccctggta tagttatatt tccacccata gttgattcat aagttatagt tccagcactt
    16741 atacctctta tagttatgta atcatcctct aatactcttg aaggagttat tgatttttta
    16801 taactacata gtacaacctt atcataattt ccagacacag ctaatcttat ttgcacctca
    16861 tcaccatctt caataacttg aataacctta ccttcaaatt ttaccttttt gtacttataa
    16921 tcatcaggtg ttctagctaa ttggtcataa gtaataccag tatcatatcc tttcttctcc
    16981 tcttcttctt ttttgcgttg ttcttcagcc tttttagctt cttcagcttt tttagcttct
    17041 tcagctttac gttgattttc agcttcaata gctttctttt cgtcgtcttt cattttgaac
    17101 catggttcag catcagacaa tttgctattt aaagtttcta tttctgtttt ttgagaatct
    17161 ataactgatg tttggttaga aatgacctcc tcatggtctt cagaacaagc tggtgaggat
    17221 atattgccaa aaaacacgcc taaaataaat gccagtagag caataccctt tgtgctctta
    17281 aagaatttaa ttattttctt agagcttcct tttccttcat tactaaattc gttaccatca
    17341 ttttttttag aaaacataat acaatctccc ttttattaga tatattttat tttaaaagtt
    17401 aaaaacttaa aataactagt tgactaaaaa ttatatattc atattttcct aaaatatatt
    17461 tcccctaagc ataattttac aaaataaagt aagaagattc aatatatttt gaaaaaataa
    17521 gataaaaatg tcagattata atataatttt cttgaaggtg aattttatat attaaattaa
    17581 acataaagta ttttatttgt ataaaactat attatgatgt aattaagtga ctcagtgatt
    17641 tagaggtggt aaaatatata tatatgaaag agcataaata aattgatata ataaaaaatt
    17701 aaatattaaa tattaactat aataaaaaaa tatctggtaa taggtataat ataaaaagaa
    17761 tataccagaa agaaggtgaa gtatgggaga actacaaaaa attccagggg tagggaaagc
    17821 aacagaaaaa tctttaatca tgttaggata tacaactatt aaatcactta aagatgcaaa
    17881 cccagcacaa atgtatgaaa aggaatgttt aatacgtgga cagcacatag acagatgtca
    17941 gttatatgta tacagatgtg cagtatattt tgcatcaact gaaaatccag aaccagaaaa
    18001 attaaagtgg tggtattgga aagattaaaa aatcaaaaaa gttagacata aaaaagagtt
    18061 gaatatccgt tttaaataat aaaaactaat actcaactct tttttagtcg taaattttat
    18121 gtaaatctat ttgtatggct aaatataccc atcaataaat gtataaatat accaatcaac
    18181 aaatgactaa acatactacc taacaaagga ttaaataaat tacttaacaa agcattctaa
    18241 aacaattaat tagaaactgt ttcttcaagc cattttctac attgctcaat atttaccata
    18301 gggtcaaaac cttggtcttt tcccttattt aaaagcatat cacaagggaa agtatcacat
    18361 tcaccacaat gattaagttc cttactttca cagcaagttt taaccttaca ttctccaccc
    18421 caaaatggtt tttccatagt tgggcaacca gtgcaattta cttgttcttt ttttgtacaa
    18481 ctgtcacaac agacaccaca tcttgattca aacatgttca aacatccttt ccattattta
    18541 aaaatattat aaaatgattt tagatgaata caaaagtgca aatagttgat tttaggtcat
    18601 ctaaatgtta aacataaaat tattagtact ggtaaattta taaaaactat tccaaaaatc
    18661 tttttgaata tttcaaaaca caataaatta cttcaaatcc ctttgaatat attttacagc
    18721 attatagatt gctttaaatc tctttgaata ttctacaaga ctatagatta gtttaaatcc
    18781 ctttgaatat attttacaac attatagatt gttttaaatc tctttgaata ttctacaaga
    18841 ctatagatta gtttaaatcc ctttgaatat ttcaaaacac catagattac tttaaatccc
    18901 tttgaatatt ctataacact ataaattact tttaactttt atataaccat aaagttttta
    18961 tctggcactt taaatggagt tttatatcaa atttgccttc catcgaataa ccaaacttaa
    19021 tttaattaca
//
LOCUS       DACULQ010000069        18990 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.069, whole genome shotgun sequence.
ACCESSION   DACULQ010000069 DACULQ010000000
VERSION     DACULQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.069"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..414
                     /locus_tag="JA154_09495"
     CDS             <1..414
                     /locus_tag="JA154_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7996097.1"
                     /translation="KYLAMDEAFPFEETVEFIKNIINKNLPQLFIIDLESDNCIGWCD
                     VLPKTEKVGYLGMGILKEYREKGIGSNLLKQIIDLSKEYGYEKIELDVFKSNSRAIHV
                     YKSLGFVEVNTISSGFTWNDRPVKEEVIQMELTLI"
     gene            complement(664..1443)
                     /locus_tag="JA154_09500"
     CDS             complement(664..1443)
                     /locus_tag="JA154_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996098.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1440..1919)
                     /locus_tag="JA154_09505"
     CDS             complement(1440..1919)
                     /locus_tag="JA154_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HAT7996099.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            2418..2708
                     /locus_tag="JA154_09510"
                     /pseudo
     CDS             2418..2708
                     /locus_tag="JA154_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2905..3912
                     /locus_tag="JA154_09515"
     CDS             2905..3912
                     /locus_tag="JA154_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HAT7996100.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            3950..5377
                     /locus_tag="JA154_09520"
     CDS             3950..5377
                     /locus_tag="JA154_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HAT7996101.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTKKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SAGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQMYMGVEE"
     gene            5519..6784
                     /locus_tag="JA154_09525"
     CDS             5519..6784
                     /locus_tag="JA154_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7996102.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTRIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            6826..8424
                     /locus_tag="JA154_09530"
     CDS             6826..8424
                     /locus_tag="JA154_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HAT7996103.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            8459..9481
                     /locus_tag="JA154_09535"
     CDS             8459..9481
                     /locus_tag="JA154_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAT7996104.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(9869..11272)
                     /locus_tag="JA154_09540"
     CDS             complement(9869..11272)
                     /locus_tag="JA154_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HAT7996105.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            12139..12648
                     /locus_tag="JA154_09545"
     CDS             12139..12648
                     /locus_tag="JA154_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAT7996106.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            12676..12870
                     /locus_tag="JA154_09550"
     CDS             12676..12870
                     /locus_tag="JA154_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAT7996107.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            12926..13282
                     /locus_tag="JA154_09555"
     CDS             12926..13282
                     /locus_tag="JA154_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAT7996108.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            13607..14137
                     /locus_tag="JA154_09560"
     CDS             13607..14137
                     /locus_tag="JA154_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996109.1"
                     /translation="MDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATLYRYLN
                     KMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQYMMGFL
                     REFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHEEGRRM
                     HSIGVIITPPKEDEGK"
     gene            14577..16502
                     /locus_tag="JA154_09565"
     CDS             14577..16502
                     /locus_tag="JA154_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide phosphodiesterase"
                     /protein_id="HAT7996110.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKYTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            16903..18807
                     /locus_tag="JA154_09570"
     CDS             16903..18807
                     /locus_tag="JA154_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide phosphodiesterase"
                     /protein_id="HAT7996111.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKI
                     FGKEVNIITTSSLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         7164 a         2145 c         3356 g         6325 t
ORIGIN      
        1 aaatatctag ctatggatga agcctttcct tttgaagaaa cagttgaatt tataaaaaat
       61 attataaata aaaacttacc acaactgttt ataattgatt tagaatcaga taactgtatt
      121 ggttggtgtg acgttttacc taaaactgaa aaagttggtt atttaggcat gggtattctt
      181 aaagagtata gggaaaaagg tatcggaagt aatcttttaa aacaaattat tgatttatca
      241 aaagaatatg ggtatgaaaa aattgagctt gatgtattta aaagtaatag tagagctatt
      301 catgtttata aatctttagg gtttgtagag gtaaatacaa tatctagtgg ttttacatgg
      361 aatgatagac ctgtaaaaga agaagtaatt caaatggagc ttacacttat ataaaataaa
      421 gttaaagtct ttaaaattcc atatacactt gtataaaata aagttaaagc ccttaaaatt
      481 ccatataaaa gaaatatttt taagggcttt ttaataatgc ttcttttgaa ggtagctaaa
      541 tatctaaaat tttatatatt aaatttaaag aaaatataaa gatggaagat acaaccatgt
      601 ttactaataa aaaaattaaa gtcataaata ataaactcat aaacaactta ttattactta
      661 tgtttatttt gcatctatta cttcatgagc cattttaact aagtcattca tagataaatt
      721 tgagtcttga actgtaaaag aatagtaaac tccatcttgt ttccatgtca aaaacttaaa
      781 tttattaact tcaactttgt ctgattcatt atttggccct acactaaatg tgtatttgcc
      841 tgatttttca tcaattttat cctgttctgt cattttataa ccttctggta caaacttttg
      901 tgcgaatgct tcataagaaa tattaatatc tttgtaagta tctacaactt ttagattgtc
      961 agaactcttc ccaagtacat tatttgctgt gtcaagagat atttcatcat tgccattttt
     1021 atattcaaaa ctaagtgctt ttgattttcc taaaacatta ttttctttgt caaatgcttt
     1081 tttatttaca gtatatgctc ctttaaattt atatccatta ctaaattcag aaactatatt
     1141 aggggtaaag ttaaagtctt tttttacttg ttctgtagtt gggatagatg tataggtagc
     1201 cttagtactt gattttccaa ccaaactata tatatgtcct actgcaaggg ctgttgtgct
     1261 caatatcaaa gtaatggcta aagttgcaac taagcctttt ctaaatggta atttgtgtgt
     1321 tttattatta atcattttat aattttcctc tctttctggt aaactattta gtgttttgct
     1381 taaactatta tgaaaacttt ctggaacttc tggaaacgaa ttatttaata tatttttatt
     1441 catatgatta cctccgtgtt ttctaattta gtttttaata atcttcttcc tcttgataat
     1501 ctacttttta cagttccttg aggtatatct aatatttcct taacttcatc tactgaataa
     1561 ccttcaatat agtaaaggac tattggaatt ttaatttttt taggtagact ttgaatagca
     1621 gtatacaaat cgctatagtc atctttttca tcaatttttt tgtttttgaa gacatcttca
     1681 aatgatagag attttaatcg tttgcgtctt aatgaattac attcatttat gactattcta
     1741 atcagccatg ttttaaagta ttgttcttcc ttaagagaat ccaacttttc atatgctttt
     1801 aaaatagcat tgttgacggc atcttcacag tcttggtcgt ttcctagaat agacttagat
     1861 actcgataca aagtctgttc tgattcaagt atattgtttg taaatgtagt tttatccaac
     1921 taagttcctt cccttctatg caaatcgtag cgctactttt ttgtctttca cttattagat
     1981 aaaattttac tatagatggt tcactttttt tatgattatt tgataaattt aagtttgaaa
     2041 gttttgccta ttctatacaa atattctata atatattttt tctagtattt tcccctcata
     2101 tataaagcca tgtagatata ttaaacttta gaagccattt atatatttag ttttatagta
     2161 agtctttata ttgagtaatt agaaaatagt aagtatttaa tgttgagtag ttagaataac
     2221 aatgcttttg gattatttac ttcatgtaca gatttaaaat aagttcttta aatagcaact
     2281 ttttgaaaaa acgcaaaaat attttatgtt gtctgattat atttatagat gtgaaacaat
     2341 attgtataga gtaaaagtat atagaagatg attacataga gtttatactt gaattttaca
     2401 aatataatta tcaaaatact gtcaaagaga aaaatagttt tgatgaacgt ttttatcaaa
     2461 ttgctacaga gacagggcta agtgctaata ctgtatataa agtattcaat aaaataataa
     2521 atagtggaaa aactgaattt acatcaaagg aattagctac tatatgtggt gtaagtatta
     2581 gaactatgga caggattata cttagattgt gtgattctgg ttattgtgag gtagtgagtg
     2641 aacagcttat gagcaagtat ggtagaccca gtagaatatt acgttttaaa ccttttctgt
     2701 tgttttaaat agatataagt caaaaattaa atttttatga taacattttc tttacaattt
     2761 tttagatata tgttaatatt tagaaaaatg catatgtatt cataaaataa acttaaaaca
     2821 gctaaaaaat cattaaaaag gaaattgtat gcatgaaatg agaaagttaa tttataactt
     2881 aaataaatct gggaggagat aattatggga aaatatcagg attcaaaaaa agttgtaaga
     2941 aattattttg aagcattaga aaatgctact ggggatgagg ttgaaaatgt cttaaagcaa
     3001 catatgaaat cagattataa ctggaaaggt gtgtatccat ttcgtgaaca agaaggcact
     3061 gaaaatgtag cagatgtatt ttggaagcct ttaaaaaaat cattgagtaa tatgcaaaga
     3121 cgacaagata ttttcattgc aggaacaaat gagattgaca atagtgaatg ggtaatgagt
     3181 atggggcatt ttatgggact ttttgataac gattggcttg gcattaaaca tactcgtaaa
     3241 atgattagtt taagatatgc agaatttaac tgtgttcaag atggtaaaat tgctaaaact
     3301 ggattatttg tagatataat tgggtttatg attcaagcag gagttaatcc attgccacca
     3361 caaacaggtt catattttgt atatccaggg ccaattgacc ataatggatt actttttgaa
     3421 gatgcagatg aagttgaagg agtaaaaact ttagcattag taaataaaat ggtagatgat
     3481 ttatctgaat taaatgaaag tggtgctatg ggatgcccac cagaaatact tgaaaaatct
     3541 tgggctaaag atatgatttg gtatggtcca ggtggaattg gtgcttctta tactatacct
     3601 agatatcaag agcaacacca gctaccattt agaaataatt taaaaggaaa gactttcaat
     3661 gggcatgtat gtagatttgc agaaggtaat tttgcttgtt tctttggatg gcctaatctg
     3721 actaatgtac catgtggagg ttttttagga ttaccaggtg gagaggttaa agcagatatg
     3781 caagttgttg atgtttatta tcgtaagggc gataaacttc aagaaaactg ggtgttaatt
     3841 gatattcctt attggttgaa acaacaaggg cttgatattc ttgagagaac taaaagtatt
     3901 tttaatgctt agttatgcat acgtattcta aaataaaggg ggatataaag tggcatctac
     3961 caacaaaaat aatgcaacaa gtaaagaaga agaaaaagaa gtttctacaa gtgggcttga
     4021 atcatgtacg gttaaagaaa acaaagctaa aatgaatcaa actaatttta gtaatataaa
     4081 tgaaaatcaa acaaatgaga ataaaacgaa cacatataaa agaaatatag ataaaggaaa
     4141 tatagataaa gggaatatag ataaaacaaa cacaaataaa tccgacataa ataaagttaa
     4201 catagataca gtggatacag ataaattaaa tacaaatact cttactaaaa aaaataaaaa
     4261 agttatattt aagggggaag ataatatgtc atctacaaaa atttactatg gagattcatt
     4321 agaagtaacg cctgttggag taatggatta caatgatttt tcaaaacaga caaaaaggga
     4381 acaggaaata cctggatttg attctaagta tagagatttt gtagattata ttatgaagat
     4441 tacacataat atctgggaag aaaaaggtat aggtgttatt tatgatacat atcacaataa
     4501 tgttacaatg cattgtggct cttcaaattt agttggtatt aaagatgtta tttccaatac
     4561 tcttcaaaca ttacatgcgt ttcctgatag aagattaatt ggtcaaaatg taatatggtc
     4621 taactttggt gctgatggtt ttttatcttc tcaccgtgtt ttgtctacag caactaactt
     4681 aggtgatagt aattttggac cagcaacagg tagaaaaata aactttagaa ctgtaattga
     4741 ttgtgccaca actaacaata gaatccacga agaatggttg gttagagata atttatggat
     4801 tgttactcaa ttaggattaa accctcagga agtagcaaag agtatggcaa aggcaagtga
     4861 atctaaagtg cttagtttac aatcaactta tggtatctgt gaatctatgg atggtcaatt
     4921 tatgccaact aagtatcaag caaaagatga ttctgctggc gaaatgatgc ttgaaatgac
     4981 aagccgtatt tataattata aatatataaa tgaagttaaa aaatactatc atgataatgc
     5041 agtagtacat tttatatgtg ataaagattt aaatggttat gatgagattc aaggaatgat
     5101 tgtaagttta cttgcatcaa ttccaaatgg aagttatgaa gtggaaagag taacttgtaa
     5161 tcaaagagaa aaaaatgaag gttatgatgt atctgtaaga tggagattac gtggaataaa
     5221 tgaaggtata ggattttttg gacagccttc tggaaagcat atagaagtaa tgggaatcaa
     5281 tcattatcat atactacaag gtaaagtaaa ggaggagtgg ataacttttg atggtatgga
     5341 tgtattaaaa caaatgtata tgggagtaga agaataaagc tgtttagagt ataaatagtt
     5401 ttgaaatata tagggaagta ttgctttata aaatttaagt ggggaatgta tatgaaaaat
     5461 agatgtttaa acttttagca aaattatgac taaatttaaa agggggaatt ataaaaatat
     5521 gaaaacaacg gttgaaatag aaaatgatat catattagga aaagactatt caaacctaaa
     5581 aagaggaatt gcattttttt caattgcttt aatatacttt ttttattgct acaattttat
     5641 ggtgggtact tttattaaac caacaatgat ttatgcttta gcagatgggg gatttggttt
     5701 ttcattaaaa caaacagaag aaatatttgc agttatgtct tttggtacaa ttccaggtac
     5761 atttattttt ggggtgattt ctacaaaaat tgggaagaag aggactttaa ttagcgttgc
     5821 tttactgatt ggattgacaa catttatacc aatgttatct cctacaaata ttatgttatg
     5881 gaaaatagca cgtttgtgta caggagtagt gttgggaggc gtatttggaa cagctatgcc
     5941 tttagttgca gatatgtttc caagtaaata tcgtggtaag ttagctgcta tattaactgc
     6001 tttattttca ttagcaatga tttttggtgg gaaagtatat ggcgttttag gtgatgcaaa
     6061 ttggcaaata ttgatgtata ctgcgattat accacctata gttggagcta tcttagcaat
     6121 tttctttgtg ccagatgatt tggagtatac aaaggaatta gttaaaaagg gaaaagaaac
     6181 tggtgaaaag attagctata taagtatgta taagggaaaa tacttatgga ttggattggg
     6241 aacgattctt ttatcaggtg caaattttac agcatattca gcattttcaa ataatgcaac
     6301 tacataccta gtgacaagaa taggaatgtc agcagcagtg gctgggtcta tatatagttt
     6361 acaaggaatt ggtcaattag taggatattt attctggggt tcaattgcag ataaatttgg
     6421 aagaaaaata ccttttatag gtatggcatt agcagcagta tttgttttta tatatacaaa
     6481 attaggtgga gataatacaa caacattcta tatgatatca gtattactag gtgttgctgt
     6541 tggatattct ggagcatggg gagcatacta cacagagttg ttcccaagta aatatcgttc
     6601 gttgtcatct ggtatgtcat ttaatggagg aaggattata tctacgtttg caatacctgc
     6661 tatagctggt acagcttctg gtactttagg tatgatgact atcttttaca tatcaatcgc
     6721 agtctttgta gcaggtgcta ttgtatggtt attcttaccg gagacgttaa ataataaaaa
     6781 ataaaatact aggaatttat tttgttactt agaaaggggt ggcttatgat ttcatcaatt
     6841 gatgtatgta ttataatagg atatttgcta ttaatgttag ttattggtta ttattcagga
     6901 aaagataata aaaatcaaga agattatttc ttggcaggta ggtcaatgcc atggattcca
     6961 attgctttat cagttgcagc tactatgatt agtgcaaatg gctttatagg tggtcctgga
     7021 tgggcatatg tagatggaat gtatccagta atggttaata ttacagtacc tttagcaata
     7081 ttttttgcac tgagcattac caccccagtt atctataaat taaaaataac ctctatatat
     7141 gaatatatgg gacttcgttt aggaaattat tcaagaggat taactattat tcaatttttt
     7201 attaattcat tgattcaagc tagttctatg gtatatattc cagtgcttat tattcaaatg
     7261 attactggct ggtcacttta tgctttagtc ccaatagttg tacttgtatc aattatatat
     7321 actctattag gtgggattaa ggcagttata tggactgata gtattcaaat gatagtagta
     7381 ataagtgctg tatttatagt aattattgta gccttaaaag gaatgaatct aagtttcttt
     7441 gatacattac aaattgcaca acagagtgga aaattaaata catttgattt cagtagggat
     7501 atttcagtga caaatacttt ttgggctact ttaattggtg gaactgtaat gtggataaga
     7561 tacttttgtt ttgaccaatc tcaagtacaa agagttttaa catctaaatc tttaaaatgt
     7621 gcaaaaaatt cttttgtagt tagtgcattt gttatgaatt tagtttacta ctttatgcta
     7681 ttggtaggag taattttatt tgttttttat gggggaagga catttgaaac ttcaaatgaa
     7741 ataatgatta cttttatttt aaatgaactt ccagtaggag ccattggaat tattattgca
     7801 ggtgtgtttg cagcagcaat gtctagtatc gactctatat taaacagtat gacaactgtt
     7861 tttacgaagg atatttatga gttttatttc aaaaaagaca acaaagaatc atcattaaaa
     7921 gttacaatga ttataactgt agttatagga gttgttatga caggttttat tattatggga
     7981 tttggaggaa gcatcaaatc aattcttgac cttgttggta actatatctc atactttgct
     8041 ggaccagcta caggtgcatt tgtactagca atgtttacat ataaagctaa tgataaagga
     8101 gtatctattg gttttattgt agggttagtt ttaggatttt ttatttctaa taaatataat
     8161 gtgagttggt tgtggaatcc agcaataggt gctacaataa cattgatatt tggatatatt
     8221 tttagtgtca ttttaaagaa aaatcataat gcagatgata ttaaaaaata cactgcattt
     8281 ggtatgagaa atcaccttat ttctcaagga atggataaag tagatggagt atctatagtg
     8341 ccattttctt taggtaaaca agaactaatt gtattagctt tttttattat ccaatatatt
     8401 atattatttt tgatacaatt ttaagaagaa atactagata tttaaggaga aatactaaat
     8461 gaatcaaaag aaagttcatt ataataaatc ggctacctca aaagatgttg caagattggc
     8521 aggagtatct caatcatctg tttcaagagc atttggtagt gcaaatggaa agggcgtaaa
     8581 acctgaagta agagaaagaa tttttcgtgt agcaaacgaa ataggatatg ttcctaattt
     8641 ggtagcacgg ggaatgatta gtggaaaaac taatgttata ggattgatag ttggtgatag
     8701 tctaggtcca ttttacaata gaattatcaa tttatttgta gaaaaaattc aagagatagg
     8761 taaacagtgt ctcgttttta aagttcctag acaacaaagt atagatagta taatttcaaa
     8821 agtaattcag tttcaagtag aagctgtgat tattacagct tcggccatga ataaggtgat
     8881 ggctgagact tgtgaagaaa acaatattcc tgtgattctt tttaatcgtt ttatacctgg
     8941 tataaaaata agtacagtct atgtagaccc aattgaaggt gggggaatgg ctgctgaata
     9001 tctgcttaag aaaggtcata agaatatagg atatattcaa tttacgagag aaacaagtga
     9061 agaaatggaa aagaaaattg gattttattc taagttaaga caaagtggga ttcataacct
     9121 taaagaagaa tctgcaaact atgattatga ttctggatat gaagcaggta agagaatgtt
     9181 agcattatca aatcctccaa cagcaatttt ttgcacaagt gacttgatag ctattggtgt
     9241 aatggatgct gcaaggtttg agtacaagtt gagggtgcca gaggatttat cagtgatagg
     9301 atatgatgat attcaaatgg cttcatggaa gagttataat ttaacgacga ttagacagcc
     9361 actagatttg ttgattgaaa aaacaattaa catacttaag tccttattaa atgaagaaaa
     9421 tccagagtca gttgttgaaa tgttaaaacc tgaattgatt gaaagaggtt ctacacttta
     9481 agtggtagat atttaattaa gtggtagata cctaattaag ttgtagatat ctgataagaa
     9541 atttttaagt tttcaatcgt tctaaattgt gagtataaaa atctactaaa tttagttatg
     9601 taaaatataa attataagtg tagaaactag gctactagct tttataccac ttcaaacatt
     9661 actggaggta gttaagttta catcagttta gttttatgta gatataatat tctgtaaata
     9721 gacaaaatta aaatatgtaa gtgtctcaaa atgaaatttt tagtttatga ggcacttttt
     9781 tgtataaaat caaatgtatt tttataactt taacaataaa aagaggctta tctctatttt
     9841 gagacagcct caaattttta aaactatatt agtttacagc ttcttttcct atcaaatctt
     9901 ttaactcttc atgagtcata gtttcttttt caaataagaa tttagaaaca gaatgtaaat
     9961 cttcaagatt atcctttaac atctctaaag tctctttata acaaatttca actatacgat
    10021 ttgcttcctt ttgaacctta tcacataaga aagtcttatc atttccatca atagtcataa
    10081 atcctaagtt actcatacca tattcacaaa ccatttggtt agctatttca gtgactttct
    10141 ttaagtcgtc ctttgcacca gtagatttat gattaaatat taattcttct gaagcctttc
    10201 cagcaagtaa tatttttatt tttccaatca actcatcctt agtataaata tatctatctt
    10261 catcaggtaa ttgaagaaca taacctaatg cttgtcctct aggaactata gatatttttt
    10321 gtataggaca aattcctact atatcactta caagagcatg tccagcttca tgatatgcta
    10381 ctattttttt ctctttttca accaatgcag agtttttaga ttctagccct gcaataactc
    10441 tttcaagtgc cttgtcaaaa tgttctgatg taatacactc actgtcatct ctaactgcaa
    10501 atatagctgc ttcatttgct atatttgaaa gatgagcccc attgaaacca tgagtttttt
    10561 tagctaaaag ttctagattt acagatttgt ctataggttt gtcatctgta tgtaccttta
    10621 gaatttcaaa tctagtgtga tagtttggtg cacctatatg aatgtgtctg tcaaatcttc
    10681 ctggtctaag taaagcttca tctaataagt caagtctatt ggtagcacct attattaata
    10741 cattggaatc tttattaaat ccatccattt caactaaaag ttggtttagt gtttggtctt
    10801 tttcgttgtt actttcaaga tgtctctttg ctcctacagc atctatttcg tctataaata
    10861 ttatacttgg agcttctttt ctagcttttt caaatagagt tctaactctt tttgctccca
    10921 cacctacata tttttcaacg aactctgaac cagtaacatt gaaaaatgat gagtttgttt
    10981 caccagctac agcagatgca agtagggttt tacctgttcc tggaggacca taaaataata
    11041 ctcctttagg tatttttgca cccatttttt gatatttttg tggtgacttc ataaaatcga
    11101 ctatttcaaa tagttcttct tttacttcat ctaatccagc aacatctcta aatgtagtct
    11161 ttggttttgt agaaagtgaa tcatctgttt gattatctct ttccattttt gtctgtgcaa
    11221 agacaggagc tggattgttg attttcttaa aaaagttgtt tagcattttc atattcacac
    11281 ctcaggtaat taattattgt taatcaattt tatattttcc atatttacta ttttttatta
    11341 tttatttttc atggaaaaaa tatacatgtt atctaaataa aaataaattt gtaagatgta
    11401 tgaaaaaatg aatttatgta tatagagatt tctacttatg tattgaataa agtattacaa
    11461 attataaatt atgtattgat tatatattga gtaaagtatt ataaagcatg tattgattaa
    11521 gtattgaata aaatattaca aatttagttg aaatgaaaat gataaaaaat cttgtactat
    11581 attttacaaa tcatataaat agagaaattg ttgtagcaca aagtagtagg tagtatatta
    11641 gaatataata taggatttgt tgtgtagtat agtagcatat tagaatataa tataggattt
    11701 gttatgtagt atagtagtat attagaatac aatatagaat ttgttgggta gtatagtgtt
    11761 atattagaat acaatatagg atttgttatg tagaatatat agagtcatgt agaatataaa
    11821 tatataaaag gattttgggt ataaatttaa aaattaatag caaaatgagt agtatagaca
    11881 tataattagt agtaggaagt aattaaattt ttcctaaaca aaaaaaatag ttgcaaataa
    11941 tttgattaag tgatatagta gataaggtaa gaataaaaca aagaagaagt ccgcttctca
    12001 ccttacgccg gaggttagta aggtaaatat aagttaaagc ttaattttaa tttaagtttg
    12061 tttaaatttg gtaacttata ttgcggatga ttacgtcatc cgctttttat tttataaaaa
    12121 ttttggaggt gtcctacaat tagcaaagaa ttagcaatca atgaacaaat aaaagataag
    12181 gaaataagag tcctttcacc aacaggtgaa caacttggag taatgcctac taaagaagct
    12241 caagcaatgg ctaatagcaa aaacttagat ttggttcaaa tctcgccgaa tgcaaatcca
    12301 ccagtatgta agataatgga ttatggaaag tttagatatg agcaagcgag aaaagagaaa
    12361 gaagctaaga aaaaacaaaa aactatagtt gtaaaagaag taagacttag accaggtatt
    12421 gaacaaaatg atttaaatac gaaagctaat aatgctataa agttccttaa aaaaggggat
    12481 aaagtcaaag ttgaacttag atttagagga agagaattag gacataaaga cataggtaaa
    12541 gaagttatgc ttaagttttt agatataata aaagagtttg gagaaccaac aaaagcccct
    12601 gcatttgagg gtaataatat ggttgtaatt atagatccaa aaaaatagat gattaactga
    12661 gaggaggaat ttgtcatgcc taaaatgaaa actcatagag gtgcagcaaa gagattaaag
    12721 aaaacaggaa ctggaaagtt aaagagagct aaagctttta aaaaacatat attaactaaa
    12781 aaatctgcaa aaactaaaat gaacttaaga aaatcaactt tagtaagtga tggagatgct
    12841 aagagaatag cacaattatt accatactaa gatacagatt gtaaataata actatagaag
    12901 agggatataa aaggaggtca taacgatggc aagagttaaa aaagctatga acgctcgtaa
    12961 aaagcataag aaaatattaa aacttgcaaa aggattcaga ggatctagaa gcaaattata
    13021 tagaccagca aatacattcg taatgaaagc attaaaaaat gcttatatag gaagaaagtt
    13081 aaagaagaga gacttcagaa aactttggat acaaagaata aatgcagctg ctagaatgaa
    13141 tggaatatct tattcaagat taatgaatgg attaaaatta tctggtattg aagttaacag
    13201 aaaaatgtta tctgaaatgg ctatacaaga cccagaagga tttgcaaaat tagcagaagt
    13261 tgctaaagca aaattagctt agtattgaat catgagatgt aaagtctttg agttatataa
    13321 ataattcaag gactttttta ttttttgaaa aatacttatt agtgtcatgt acaaccaatt
    13381 ttataatgat aaataaaata agcaatacaa tagaatttta tgagatttat tataagaatt
    13441 gttacaatta tttatgatag ctataaaaag tcatagtaaa aatgatattg atgtatgttg
    13501 atatatgtat aaagctttca atcaatcttg aattaatatt atcaacatga taatattatc
    13561 ttattaataa tataaataag ggggagataa tatgaatgaa aaactaatgg attgttttgt
    13621 aaacccaatt aaatgcaagt tattgattga aattttttca aataatcgaa ctacagctaa
    13681 acaattagga gaaaaatttc aagatatatc aaaggcaaca ctttatcgtt accttaataa
    13741 aatgttgaaa gatgatatta taaaagtagt tgaagaaaat aaaataaggg gaactgttga
    13801 aaaagtatat gctatgaagt tagatatagc tgatgatgtg aacaaaatta tagatgagaa
    13861 ctctggagaa gaatatgctc aactgtttac tcaatatatg atgggatttt taagagaatt
    13921 ttatgaatac gctgaaagaa aggaaaagga tatcctaaaa gatggttcag gattttctgt
    13981 ctgtccagta tatgctagta ttgatgagat agttgaagtg agtaaaaaaa ttgctagtat
    14041 actagaacct ctaagaaata actcgcacga agaaggcaga agaatgcatt ctataggagt
    14101 gataatcact ccacctaaag aggatgaagg gaaataaatc tgatttacat gaggtagagc
    14161 caatttacac ggaaaatcgt tttatgattt acatatgtat ccagagcaaa gtatctctgc
    14221 ttggattgta cttcattgta tcatttcatc tatctaatga cttggagtgg tacaatgaag
    14281 ttttttaatt tttcatatca atgcctaaag aaatgaactt tagaccacat atgcaattag
    14341 gttacattat acaattagac cagatataca aaataaaaat cagtattttt atagcagata
    14401 aattgactgt tattcttctt aaaaatcatc tattagttat tgttagcata ttgaaagaaa
    14461 ttataggaat ctattgaata aatgaatatt gcccataatc atataaactg ctatagttta
    14521 aaataaagca ggacgttaaa ttttatagta atctaaaaat ttaagagggg gattttatga
    14581 aaataaaatt tgttattatg ccattggtga tatttttaat ggtattattt gtggggtgtc
    14641 aaagtacaaa agatacagaa gttaaagcaa aatatacaga aattaaaata ttaggtacag
    14701 gtgatttgca ttcaattttg acagactcta tggtttcata tgtaaatgag gaaagggaaa
    14761 agaataaaaa tctgcttatg gtagatgcgg gagatttcta tggtacacaa agtagagaaa
    14821 tgtgggaatg gtcttctgga aaaaagttaa ttaatattaa aagaaatggt agagcagagt
    14881 atgatgacat aattaagagt agtaaagatg aagtacctat agttaaagat atggcaaagt
    14941 tgaaatatga tgctgtagtt ttgggagata atgaatttgt ttcaaatgat aagcaaagtc
    15001 ttgataaact agtttctgat tttaaaaata ataatatgcc attggtatca gcaaatatat
    15061 atgaacaatc tggagaaaat tatgtacaac catatgtaat gaaaaatatt aagacagatg
    15121 agggcaatgt aaaagttgga atactaggtc ttactataaa agaagtaggg gaaacccttg
    15181 gtcttgagga ttttgaaaag gataaaaaag ctagagagct tggagaacaa gctgattaca
    15241 aaggaaaatt atatgcaaat gatttagtag aagatgcaaa gaaatggata aaagttatgg
    15301 aaaaagaaag tccagacata atagttgcag ttgtacactc aggggaagaa cctaaaacag
    15361 atagaaatcc aggaaataga ataaaagagt tagcaactac agtggatgga atagatgcaa
    15421 ttgtagctgg tcatactcat gaaaaaatag atgaacacaa atataaaaat aaatctggag
    15481 atgaagtaat tgttacacaa ccaggttggc atggagatag agtatcagag attaatttca
    15541 aattaaataa agagaatgga aagtgggaca taaaggaaaa gtctagtaaa ttaaaaataa
    15601 ttgataaaga agtaagtata gttgctactg cagatttaca ttcacatttc tctgataaat
    15661 taacagttga ccttgttaat gaacgtagta acccaattga acctgttgta gtagatgcag
    15721 gcgacttctt agaccctcaa acagatgaaa tgactcaatg gtataaagag tggaaaagta
    15781 taagagataa taatagtgat aaaacgatta gtagatgccc aatggtcatg gatatgaatc
    15841 agggaatgta tgatgcagta gtacttggaa atcatgaatt tgtatccgaa aaagatgaat
    15901 tttggtcaga tgaacgcctt ttagatgctg ttatagagga ttttgaacag gtggcaattt
    15961 ctgtgttatc ggcaaatata tataggcaat ctggtaaaaa ttatgttaaa ccatatatta
    16021 taaaagatgt ggagacgagt gaaggtaatg taaaagtagg aatattggga cttactataa
    16081 aagaagtagg taaaggtctt aaaaatgtaa acttacaaga gcaatttcaa tataaggata
    16141 aattgtatgc aaatgattta gtggaagatg caaagaaatg ggtaaaagtt atgaaaaaga
    16201 aaaaaccaga tataatagtt gcagttgtac attcaggaga agaaccaaaa aatccaaaac
    16261 atccaggaaa tagaataaaa gaactagcaa ctacagtaga tggaatagat gcaattgttg
    16321 ctagtcatac tcatgaaaaa atagatgagc atgaatataa aaataagtct ggagaaaaag
    16381 ttatagtgac acaaccaggt gagcatggta aatattattc taaaattacc tttaaagtaa
    16441 ataaagaaaa gggttcgtgg attattaatg ataaattcag taaaacaatt gaggttgaat
    16501 aactttataa aatttataga taaagtaaat tatctgatat tgacatgata tataagataa
    16561 agtttctaat attagtataa tgcataatgt gaaatatttt aataataaga gaataaacaa
    16621 gacaaataaa aatattttaa catgtaagat atagtatgaa ctattatact aatgcttact
    16681 ttataatttg aacatgttag aataagtctt atttgaaaat tttagtgtat tagagtaagt
    16741 attattttac cagtaaaaaa tattgagaac tatatgtaga tttgcttaaa aaggaattta
    16801 catatagttc ttttcgtata ataaatagta caatcttata tggtaaagat aaagtgtgat
    16861 attaaatttt attatcatcc aaaatctaag gaggagtctc ttatgaaaat aaaatttatt
    16921 atagtagcat caataatatt tttaatagta ttacttgtgg ggtgtcaaag tactaaagac
    16981 acagaaatca atgtactcgc cacaagtgat ttacattcaa ttattccaga taatttaatt
    17041 tcctatgtag aagaggagag aaaatatgat gagaatctac ttatggtaga tgcaggagat
    17101 ttttttgaca tgcaaagtgg tgagatgaat aagtggttta ctggtcaaaa attagtaaga
    17161 tttaaagatg ggataccaga gtacaaaaaa atagctgaac cacgtgaagg agaagttcca
    17221 ttggctaaaa aaatggcaaa gctgaagtat gatgcagtta catttgggaa tcatgaattt
    17281 gtatctaatg acaagcagag ccttgataaa ttggtttctg attttaaaaa taacaatata
    17341 ccattagtat cagcaaacgt atatgaacaa tctggagaaa actacgtaaa gccatatgta
    17401 atgaaaaata taaatactga caagggaact gtaaaggttg gtatacttgg tctgactata
    17461 aaagaagtag gggaacgttc aaggtttaaa agttttgaac aagataaaaa ggctagagaa
    17521 cttggggaac aacctcaata caaagggaaa ttatatgcaa atgatttagt agaagatgca
    17581 aataagtggg taaaggttat ggaaaaaaaa gaaaagccag atataatagt tgccattgta
    17641 cactcaggtg aagagccaaa aaatcctaaa aatccaggaa atagaataaa agaactagca
    17701 acaactgtag agggaataga tgctattgta gcaggtcata cacatgaaaa aatagaacag
    17761 catacatata aaaataattc tggtgaagaa gtaatagtaa cacagcctgg agctcatggt
    17821 aatagtatat caaagattaa ttttaagttg gataagaaga atgataagtg gtttataaaa
    17881 gacaaatata gtgaattaaa gatttttgga aaggaagtaa atataattac aacttctagt
    17941 ctgaatccaa aattttcaga tgaaatggca gttaatttgt ttaatgaaag agatagagat
    18001 gttcaaccta tatttataga tactggagat tttttaaata ctaatacaaa agaaatgact
    18061 aagtgggcta aagagtggca atacaatgaa aataaaggca tctataaaaa aataaatgaa
    18121 tggccaattg tatatcttgg ttatgatgct gtagtacttg gtagccatga atttacagag
    18181 gataaagaga cttttgagga aaaaaaatct acattggatt atattataga aaactttgaa
    18241 gaaatgaaga taccagtatt atcagctaat atatatgatg aatctggaga aaattatgta
    18301 aaaccttata taattcataa tgtagaaact agggaaggaa atataaaggt tggagtatta
    18361 ggtcttacta ctactgataa ggatgaagaa gacttaagta gctcaagctt aagcaaagac
    18421 tcaaagactt cgaaaattcc tgaagaaaca ggaaagttat atactaataa tctagtaaaa
    18481 gatgcaagcg aatgggtaaa agttatgcaa aaggaaagtc ctgatgtgat tgttgcagtt
    18541 gtacattcag ataataatag tactctaaaa aatccaagta gtgaagtcaa aaaacttgca
    18601 atgtcagtag atggaataga tgctattgtt gtaggtcata cgaaaaataa ggtagaagaa
    18661 catatttaca aaaataagtc tggggatgag gttatagtaa cacaatctgg agaagattgt
    18721 tattcaaaaa taagtcttga actaagaaaa gaaaatggca agtggaatat cataaacaaa
    18781 tatagtaaag caataaaaat gaagtgaatt tattgatata taaaccatta ttaacactga
    18841 tttttataat ataataaata ctgattctat aatataatta acactacttt ttataatgtc
    18901 atcaacactg attctataat ataattaata ctactttttt aaatataatc aatactgatt
    18961 ccataatata attaacacta cttttttaaa
//
LOCUS       DACULQ010000070        18619 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.070, whole genome shotgun sequence.
ACCESSION   DACULQ010000070 DACULQ010000000
VERSION     DACULQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.070"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1126
                     /locus_tag="JA154_09575"
     CDS             <1..1126
                     /locus_tag="JA154_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HAT7996112.1"
                     /translation="NEILRNLTKIIKRNIFEEEMFARISADNFIIIMKYIEEEDITKR
                     LKTIFEELDLFNNNQEEKYKLVLSCGIYFILPEDRDINSIIDRANIPHKMAKGGHKSS
                     YAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIYLQPKIELNTGEIQGSEALVRWKRP
                     DKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIYLRKWIDAGINPVTVSVNVSRIHLY
                     CNNFIETYKNIIDKYNIPAKYIELELTESIIFDNFDILIDIMNNLKKIGFLISMDDFG
                     SGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSKIIVTKVIEMAKELGMKVISEGVET
                     EEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIVSNLVRG"
     gene            1302..1670
                     /locus_tag="JA154_09580"
     CDS             1302..1670
                     /locus_tag="JA154_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7996113.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            1672..2397
                     /locus_tag="JA154_09585"
     CDS             1672..2397
                     /locus_tag="JA154_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996114.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2391..3155
                     /locus_tag="JA154_09590"
     CDS             2391..3155
                     /locus_tag="JA154_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996115.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3377..4627
                     /locus_tag="JA154_09595"
     CDS             3377..4627
                     /locus_tag="JA154_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7996116.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNIDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            4820..5704
                     /locus_tag="JA154_09600"
     CDS             4820..5704
                     /locus_tag="JA154_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAT7996117.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            6063..6680
                     /locus_tag="JA154_09605"
     CDS             6063..6680
                     /locus_tag="JA154_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996118.1"
                     /translation="MCKKMYLLVVGILIAGLLTACNGSPTKDSEQAQDNTKKEANVSN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDFNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            6788..9319
                     /locus_tag="JA154_09610"
     CDS             6788..9319
                     /locus_tag="JA154_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT7996119.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDEDEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            9753..10454
                     /locus_tag="JA154_09615"
     CDS             9753..10454
                     /locus_tag="JA154_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanS"
                     /protein_id="HAT7996120.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            10444..11586
                     /locus_tag="JA154_09620"
     CDS             10444..11586
                     /locus_tag="JA154_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HAT7996121.1"
                     /translation="MKSKNIKVNPDFTQVRNKIFMRTALMAFIAIIFIWILYSFILYG
                     KFSIWIVSFFENAFRMEYKAALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTK
                     YFSEINQGIDSLIKEDVGEVALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELI
                     VYLAHDLKTPLTSVIGYLTLLRDESQISEELRKKYLSISLDKAERLEDLINEFFEITR
                     FNLSNISLEYSTVSLTRMLEQLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVF
                     DNLLRNAVYYSFEDTTIEITAVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSR
                     NSNSGRAGLGLAIAKEIVELHNGTITAHSENNIITFKVIIPIVGNL"
     gene            11847..12947
                     /locus_tag="JA154_09625"
     CDS             11847..12947
                     /locus_tag="JA154_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase"
                     /protein_id="HAT7996122.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            12944..13750
                     /locus_tag="JA154_09630"
     CDS             12944..13750
                     /locus_tag="JA154_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAT7996123.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            13768..15906
                     /locus_tag="JA154_09635"
     CDS             13768..15906
                     /locus_tag="JA154_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid racemase"
                     /protein_id="HAT7996124.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
     gene            16220..16840
                     /locus_tag="JA154_09640"
     CDS             16220..16840
                     /locus_tag="JA154_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAT7996125.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(17006..17542)
                     /locus_tag="JA154_09645"
     CDS             complement(17006..17542)
                     /locus_tag="JA154_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAT7996126.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(17655..18359)
                     /locus_tag="JA154_09650"
     CDS             complement(17655..18359)
                     /locus_tag="JA154_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAT7996127.1"
                     /translation="MKKKILIPVIMSLFIISQCITSFAFTPENNKFKVKPLPYAYDAL
                     EPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVR
                     NNAGGAYNHKFFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWA
                     WLVATKDGKLSIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVV
                     NWNGALENYKNLKSQD"
BASE COUNT         7078 a         1910 c         3228 g         6403 t
ORIGIN      
        1 gaatgaaatt ttgcgtaatt taacaaagat tattaagagg aatatatttg aagaggaaat
       61 gtttgcaaga atatctgcgg ataattttat tataattatg aaatatatag aagaagaaga
      121 tataacaaag aggttaaaaa ctatatttga agaattagac ttgtttaata ataatcaaga
      181 agaaaaatat aagttagttt taagttgtgg aatctatttt atacttccag aagatagaga
      241 tatcaactca atcatagata gggctaatat acctcataaa atggcaaagg gaggacataa
      301 aagttcatat gcattttatg ataataagat tcatgaccaa gagataaaag aaaaagagat
      361 ggaaaataca atgttttcaa gtctagaaaa taaagaattt attatatact tacaacctaa
      421 gatagaactt aatacaggag agatacaagg ttcagaagca cttgttaggt ggaaacgtcc
      481 tgataaaggg ctaatacctc caaatgaatt tatcccattt tttgaaagaa acggttttgt
      541 tataaattta gatttatatg tgcttgaaga agtatgtata taccttagaa aatggattga
      601 tgcaggaata aatccagtta ctgtttcagt taatgtttca aggatacatt tatattgcaa
      661 taattttata gaaacataca aaaatataat tgataaatac aatatacctg caaagtatat
      721 agaacttgaa ttaacggaga gtatcatatt tgataatttt gatattttaa ttgatataat
      781 gaataattta aaaaaaatag gatttttaat atctatggat gattttggct cagggtactc
      841 atcattaaat atgttaaaag aaattcctat ggatatctta aaacttgacc aaaaatttat
      901 tatggaaact tataatagta aaagaagcaa aataattgtt actaaagtaa tagaaatggc
      961 aaaagagttg ggtatgaaag tcatatcaga aggagttgag acagaagaac agtttaaact
     1021 tttaaaagaa gtaaagtgtg atatggcaca aggatatttg tttggaaagc caatgccaat
     1081 tgaagaattt gaacatttaa ttgtatctaa cttggtaaga gggtaattaa tctgtaatat
     1141 aaagaattta ataaattgtg ttaaaaatgg actctaatgt agtgttcatt ttttttgtaa
     1201 aattttttat acttttacta aaaagtaatt gacatatact ttgtattatt atattattgt
     1261 attaacaact taatacaata atataataag gaggcatgaa catggaatgg gaacttgata
     1321 ataacaaacc tatatatata caattagtag aacatttaaa gttaaaaata atttcagggg
     1381 aaattaaaat aggatctaaa ttagaaacag tgagagcact agcagaagat gcggaagtta
     1441 atccaaatac aatgcaaaag gctctaacag aacttgaaag acaaggttta gtttatagtc
     1501 agagaactaa aggaagattt gtaacagatg ataaggaaaa aataaaagct atgaaagagg
     1561 aaatagctaa tgtggagata aatacattaa aagtaacctt agaaaagctt ggatatgata
     1621 gagatgaaat gctaaaactt attacagaaa atctgaaagg agaattataa aatggataaa
     1681 aatataagca atgtttctca agatggaata gttgaattta aaggtgtaaa caagagttat
     1741 ggaactaaga atgttttaaa aaatattgat ttaaatatac caaagggaaa aatagtcggg
     1801 ctgttaggac ctaatggaag tggcaaaagt acaatgatta agctaatgaa tggattatta
     1861 cagcctgata atggtgaaat tatgataaat ggaatgaaac catccataga tactaaaaaa
     1921 atagtttcat atttaccaga aagaacttat ttaaatgatt ggatgaaggt atcagattta
     1981 cttaagtttt tctatgattt ttatagtgat tttgatgtta gaagagcaaa tgagatgatt
     2041 aaaagcttag atattgatgt aaatgaaaag ctaaagacaa tgtctaaagg aactaaagaa
     2101 aaagttcaac ttattttagt aatgtcaaga aatgcgagca tatatatatt ggatgaacct
     2161 ataggtggtg ttgacccagc tgcaaggtca tatatactta agactatatt aaaaaattat
     2221 tctgaagata gtactttact gattgctacc catttaataa gtgaaataga aaacatatgt
     2281 gatgagataa tattcatatc aaaaggagaa atagtgttac aaggagacgt tgaagcaata
     2341 agagaagaaa aaggaaaatc tatagatgca ttatttaggg aggaatttaa atgttaggta
     2401 aattattaaa atatgagtta aaagcaagtg gaagaatatt tataccgtta tatatagcca
     2461 tattgatagt agctgtattt aacggaatat ttatgaatac aaatatactt caggtacagg
     2521 gaataggaat actagtatta acaagtcttt ttatggcatt aggagtttta actattgtag
     2581 ttacaattca aaggtttaga aaaaatcttt tgggggatga aggttattta atgtttactc
     2641 ttcctgttag cactagttca cttatattat ctaaatgtat aacagcactt atatacgctg
     2701 tacttagttt tatagttgca gtattcactt ttggagttct tatgcttttt ggtacttctg
     2761 gaattttact acctgaaatt ttagatttgt ttaacactag ttttaaatgg atttctgaaa
     2821 actttttaga tattttatta ttagtagtag taatgtttat ttcatattca tcatttattt
     2881 tgttattata tacatcaatc tcaatgggac aattaccaaa attcaataaa catagaaaca
     2941 tagtggcatt tgcttctttt atagctataa atatagtaat atcaattgtt ggagatgcag
     3001 ttggaagcat cttaccaaat gaagatacaa atatggtata tcatctttat cagagtccta
     3061 gttttatgct tgcaatactt ggtagtcttg tagttgcaat agcgttattt ttcgctacaa
     3121 aatttatatt agataaaaaa ttaaatttag aataaatgtt tttatatgga gccatctttt
     3181 gagtaatgtt aaaagatggc tcttattatt tatgtagtta attcatgtat ataatatgaa
     3241 tagtgtttta tttctagtta aatatataca tatgttgtat aatgatattg cgtgtaaaaa
     3301 tttgtaagaa taactataca ctttatataa gttatatcaa gaaaatttaa aaatatatgg
     3361 atttgaggga gaagatatga aaagttatac aaaggctaag tggagtgttt ggggtataat
     3421 tacattttca tttgttttag tattattttt aaggatgtca actgctgttg tttcagacaa
     3481 tttagcaaat gagttaggtt ttaattctat acaaatttca aatatagcat ccttttgcct
     3541 atatgcatat gcatttatgc aaatacctgc aggaattttg attgataaat atggagcaag
     3601 aaaaattagc agtcttggaa taataatggc aagtttaggg tcaattttat ttggtttgat
     3661 tcaaagtata gaattagcat atatttctcg tgtaattgta ggggcaggaa catcagtaat
     3721 attactttgt attttaaaaa ttcaagggag atggtttaat aagtcagaat ttgcttctgc
     3781 aacagctaaa ttttcatttg taggaaattt aggtggagtt cttgctactt ttccacttgt
     3841 gtttttgtct gaacttgttg gttggagaaa ttcattttta ttaatcggta taattggtgt
     3901 tgtaattgga tgttttatgt atattattgt tagagataca ccaaaagagt atggattcaa
     3961 tatagatact gaaccctatg aaaagtcaga gaaggttaat attgttgatg gtattaagtc
     4021 tgttataaag aacaaatcaa catggtataa ttcaatgata atgttctctt ttgtgggtct
     4081 tacaagtgca tttataagct tgtggggagt aagatacata atggacgttt atggtgtgag
     4141 taagagtttt tctgcgttta tagtatcatt ttttacatat ggatttatat ttggttcaat
     4201 aattatggat tttgtatttg ctaaaataag gtctagcaaa tttaatataa taaaatttgg
     4261 ggcaatgata gatttgttta tatggattgt aattgtagtt gtataccagg taaaaccacc
     4321 aattatagtt ttaccaatat cattctttat tatgggatgt atagttatgt cacatctaca
     4381 ggtttttaat gatgccaagt ataaaaataa agaaatttat tctggactgg caactagtgt
     4441 tattaatact tttgaattta taggaagtgg aattattaat ttgattatag ctatctcttt
     4501 acaagtaaat tcatataata tagttgatgg atataaaaaa ggttttgtag tatttatagt
     4561 actaagtatt ataactatag tatcatctca tataggggta aaaaatgatg attttaaggc
     4621 tatatagtgt tttattaata gtttaattta gtattatatt ttgcatatat atataacaaa
     4681 tagtttgcat tttcctaaga tgtataattg gaatataaat cttgtttact atgtacaaat
     4741 ggtattgtat tatattaaat agaaaataag aactttaaac tataatattg gttctaaaat
     4801 ataaggtaag gagtttttta tggatacgac gaaaatagat gatataagca agttagattt
     4861 aaataattta gatattaata agatggatat gggaggtata atggaaagtc tattagaatg
     4921 ggctactaca agcggagtaa aacttataat aggcatactt atactttcta ttggatttaa
     4981 aataataaaa aaatttgtaa accatgttat gttattactt agtaaaagag atattgattt
     5041 aactttaaga aggtttttga agtctcttct tttgagtgta cttaaagttg ccgttataat
     5101 cattgttctg gaatattggg gaatgagttt gtccagtttt gctgctgtga tagcatcagc
     5161 aggggtagca ataggtttgg ctttacaagg tagtctatca aattttgctg gaggatttat
     5221 tatactattg attagacctt ttaaagtagg agattacgta gaagcagcag gccatggagg
     5281 aacggttgaa caaataggac ttttttatac acaattggtt actccagata ataaacagat
     5341 acttatacct aatggttctg tatcaaatga tagtctaata aactattcag caaagaatac
     5401 tagacgagtt gatttgactt ttagtgtagg gtatgaagat gatatactac atgttagaag
     5461 agtacttaaa gatattgtta ataatcataa attaataata aatgaaccag aaccatttat
     5521 aggagtagta gaacatggag ataatgctat taaatttgca actagagttt ggtgcaagac
     5581 agaagattat tggacaattt atcttgattt actagaagag gttaaagtta ggtttgacga
     5641 agaaggaatt acgattccat atcctaaaat ggatttaacg gtaaaagaat taaataaaat
     5701 ttaatttttt tatgtagtgt ttaaatttaa gaatattata tagatatatt tataagattt
     5761 gtgaaaaatt aataagtcct gttattttgt aagtgaaaat ataaaaaatg ggagtgtctc
     5821 aaaatgaact ttttagttca tgaggcactc tttttgtatg aaatcaaata tatttttatt
     5881 atttcaacaa taaaaaagag gtctgtttct attttgagac aatattattt ttatgtagat
     5941 ttaatcttaa tgagtataaa gttttaaaac tactaaaata ggaggaattt taatataaaa
     6001 gggtaaataa agggtttaga gggcataata tagaataaat taattattta aggagggttt
     6061 ttatgtgcaa aaaaatgtat ttattagtgg taggtatact tatagcaggt ttattaactg
     6121 catgtaatgg gagtccaact aaggattcag aacaagcaca agataataca aaaaaggagg
     6181 caaatgtatc aaataacgct ttaaaagatg aaaaaaataa tgaaaattta atggaacaag
     6241 attttaaagt gccatacaca gatgcaataa acatatttaa agacaaatac aaagatgctg
     6301 atatagtaga tttaagtctt gagagagatt taaataaatt tgtttatact gtggaaggtg
     6361 tagatgataa taatgaatat aagatgaaaa ttgatgctaa tacaaaagat gtattagaag
     6421 ataaaacaga aaagttggat tcagaagatt taaatggagt cgctagaaaa gaaaaattag
     6481 attttaatga cataatgaca cctcaacaag ctatggaaat tgcattaaaa gaacaaaatg
     6541 gaatggttaa agagtggagt ttagataaag atttagatgt tactttttat aaaataagaa
     6601 tagataaaga taaaaatgaa tatgacataa aagtagattc taaaaaggga actgtattga
     6661 aggtagaaaa agaagattag aagatttaaa ctatctttaa aataatttct tttttgaata
     6721 aaatttgttt aaaatataac aagtagggta taaagagact aaataactat tttaggagga
     6781 gtacggtatg tcattgaaat cgctaaaaat aaaagaaaat ttatattggg taggttcgct
     6841 tgaccctgat ttaagagtat ttgacataat tatgtataca ccttatggaa ctacttataa
     6901 ttcatatgta ttaaaaggaa cagaaaaaac agttttattt gagacagtta aagataagca
     6961 ttttgacaat tatattgaaa gattaaatga tttaaatata gattttgaaa aaatagacta
     7021 tattgttgta agtcatactg aaccagacca tgcaggaagc gttgaaaagc ttttggattt
     7081 agctaaaaat gcaaaagtag tagcttcaga aacagcaatt aaatatctta aggaaatcgt
     7141 aaacaaagac tttgaatatg ttgcagttac agatggagat acattatcaa ttggggacaa
     7201 aactttagaa tttttctctg tacctatgct tcattggcca gataccatat acacatatat
     7261 aaaagaggat aaaactcttg taacatgtga ttcatttggt agtcactata gtaatgataa
     7321 gatagtaaat acacttgatg aagatgaaga aaaggattac ttggatgctt tgagatacta
     7381 ttatgattgt ataatgggac catttaaacc atctatggta actgccatag aaaagataaa
     7441 agatttagat atagatactg tatgtccagg tcatggtcca gtattaactg aaaatcctag
     7501 aaaaattata gacctttatt acaactggag tgtaaatgaa caaataaagt tagaaaaaga
     7561 agttacaatt tgttatgttt ctgcacatgg atatacaaag attatggcag aagctataaa
     7621 agcatatatt gaaaaaaata gcaactataa agtaaattta tttgatgtaa tagagcataa
     7681 gcaagaagat attttggcaa aaatagcagt atcacaaggt gtattatttg gaacaccaac
     7741 aatattagga gatgcgttaa agcctatatg ggatatatta atatcgttaa acccagttct
     7801 tcatggtgga aaagttgctt cagtatttgg ttcatatgga tggagtggag aaggtataga
     7861 aaatgctatg gagagaataa gtcagcttag aatgacagca gtaaaacctt ttgctgttaa
     7921 ctttaaacca tcaaatgaag agattgataa attacattct tatacaggta aattcctaga
     7981 taagttaaat tctacttttg gaagtaaaaa gaaaactaag aaatttaaat gtgtaatttg
     8041 taatgaagtt tttgaaggtg atagtgctcc tagtgtatgc cctgtatgtg gagctaagga
     8101 agaccaattt atagaagttg aagaagatga agttactttt agaaaagata ctgatgaata
     8161 tttcgtaata gtaggaaatg gagcagctgg tttttatgca gcagatgcta taagaaaaag
     8221 aaataaaact tgtaagataa ctatgatttc taatgaagat gagttgactt actatagacc
     8281 tgcactttct gatggtataa atgaagaact tggttcagat ttctacatgg aagataaaga
     8341 ttggtatgat aaaaataata tagtagtaat attaggaacg aatgtagata aattagatga
     8401 agtgaataaa actattatag tgaatgatgg agctattaaa tttgataagc ttgtaatagc
     8461 tacaggaagt agaaacttta taccaccaat taagggtcat gatttagaaa atgtgtttac
     8521 tcttagaaat ataaaagatt tatatagtgt aaaagaagca ctagaaaaat ctaagaaagt
     8581 tgtagtgatt ggtggcggtt tattgggact tgaagctgca tgggaattta gactaaaagg
     8641 attagaagta gtagtagttg aagccatgga tagcatacta tcaaaacaac ttgataaaga
     8701 aggcagtaaa atattagaac aatgtgtaag agatacagga atagatgtaa gattgggtgt
     8761 agcagttgat ggtattgaag gtgatggaaa agctcaaaaa gttgtattta aagatggaga
     8821 tagtgttgac tgtgatatgg tagtattttc aattggtgta agagcaaaca ctcaaatggt
     8881 tcaggataca tctgtaaaaa ttgacagagg tattgttgta gataagacat tacagacaaa
     8941 tgttaaagat atatatgctt gtggagatgt tgcacaagtg ggaaatatta gtttagcaat
     9001 atggccatct tcagtagaaa tgggaaaaat agcaggggca aatgcaagtg gagataattt
     9061 gacatttgaa agtgaagtat atccagtttc attagatgca atgaatgtaa aagtatttag
     9121 tattggtaat atacaaaatt ttgataaaga aatttcatca aaagatgaag gtcaaagaat
     9181 atataaaaaa ttatttatga aagatggttc attagttgga gctatactta taaatgattt
     9241 aagctgtact gttaaattga taagattaat atctgaaaaa ggtgattttg aggatattat
     9301 gaaggctgat atactataat ttaaatacat taaaataaaa aggagttgtc ttaaaattga
     9361 tttttttcta atcaaatttt aaattcaact ccttttttga atttttatat aaaataacat
     9421 ggaaatttta gtaatgtctg tggttacata cataggttta attgtttttt atatggattg
     9481 tctctggtat aataaattga attgtgtttg actgatagat atttttattg aaagttaata
     9541 cctgttatgc gataataagg acgtgttagg ggagttttta taataacaat taatctcagt
     9601 ttttgtgttc caatagtagc atcaacacat tctgatgtag tagttctttt cttataaaaa
     9661 ttagttacaa atatgcttgt aattatttga tatgtaaatt taccaatatt ataaatgatg
     9721 acctcgataa taaggagagt gaaaaataat ctatgaacga aaatattttg attgtggatg
     9781 atgaaaaaga aatagcagat ttaattgaag tatatttaaa aaatgaaggt tttacagtat
     9841 acaagtttta tacaggtaag gaagctctag aatgtattga atctaaaaat ttggatatgg
     9901 ctattttaga tattatgctt cctgatattg atggatttca tatttgccag aaaattcgag
     9961 aaaaatacta ttatcctatc attatgttaa cagctaaaat tgaagatata gacaagataa
    10021 tgggtttaac cataggtgct gatgattata taacaaagcc atttaaccct ttggaagttg
    10081 ttgcaagagt aaagacacaa ttaagaagat atatgcgata taataattct tatgaacaac
    10141 agtcgataat tgttaatgag tatgatatta aaggattgat tatcaataaa gaaacacata
    10201 aatgtagttt atttgggaaa gaagtagcat taacaccgat tgaattttct attctttggt
    10261 atttatgtga acatcaagga aaagtggttc cttctgaaga attgtttgaa gctgtatggg
    10321 gagaaaaata tcttgataat aataacacag ttatggctca tatagggcga ttacgtgaaa
    10381 aaattcatga accttctaaa aacccgaaat ttataaaaac tgtatgggga gtaggttata
    10441 caattgaaaa gtaaaaatat taaagtcaat cctgacttta ctcaagtgag aaataaaatt
    10501 tttatgagaa cagctttaat ggcatttata gccattattt tcatatggat tttatattct
    10561 tttatattgt atggtaagtt ttctatctgg atagtatcat tctttgaaaa tgcttttcgt
    10621 atggaatata aagctgcatt gaatttatat cagcagattt tccgtaatag tatggagctc
    10681 ttcatgatac tagctatggt gattgtgttt tttataatat tccgtgttta tttaaattgg
    10741 tttacaaaat attttagtga aataaatcaa ggtattgact ctcttatcaa agaagatgtt
    10801 ggagaagtgg ctttatctcc agagttattg gcaatagaaa agaaaataaa ctcaattaaa
    10861 catatacttg aacaacgtaa atttgagaca caaatggcag aacaacgtaa gaatgaactt
    10921 attgtttatc ttgcacatga tttaaaaaca ccacttacat cagttatagg gtatctgaca
    10981 ctacttcgag atgaaagtca gatttctgaa gaactgcgaa aaaaatattt atctatttcg
    11041 ttggataagg cagagcgtct tgaagattta attaatgaat tctttgaaat tacaaggttt
    11101 aatttatcaa atatatcact tgaatacagt acagtaagct taactcgtat gcttgaacaa
    11161 ctaacatatg agtttaaacc tactttgatg agtaagaatt taaaatatac actgaatgtt
    11221 acaccaaata tgatgataag gtgtgatgtt aataaaatgc aacgtgtatt tgataactta
    11281 ttacgaaatg ctgtttatta tagttttgaa gatactacta ttgaaataac agctgtacag
    11341 gaggatgatt atataaagct taaatttata aatcatagca atacgattcc agaggaaaaa
    11401 cttgagcgta tttttgaaca attttacagg ttggatacat ctcgtaattc aaacagtggt
    11461 agagctgggt tgggacttgc tatagctaaa gaaatagtgg agcttcataa cgggacaata
    11521 acagctcata gcgagaacaa tataattacc tttaaggtta ttattccgat tgtaggaaat
    11581 ttgtaagaat ttaagtataa aaaaatagga aattctttag gataaataaa taaagagcac
    11641 tcttgttatt gatataatta ctgtatcaaa acaagagtgc ttttattttg atacagtaat
    11701 tatatcaata gaaaaatgtg aaaatatatt atgtggaaca gatgcaattg taagtgaagg
    11761 ttgattggat ttgaaataga aaaagtattt taacatgttt ctgtagattg tataaaaata
    11821 aataaatcaa caaaggagaa tcaaagatga ataataaaaa aattgcagtt atatttggtg
    11881 gaaactcaac agaatatgat gtatcattac aatcagcttt tgcagtactt gagaatttga
    11941 atacggaaaa atatgatatt ataccaattg gaattacaag ggagggtaat tggtatcatt
    12001 atattggaga ttataataat atccagaata acacttggtt tgaaagggat actgatataa
    12061 ttccagttat tgtttcgcag aaccgtgtca aaaatggtat tattgaattt ttaattcctg
    12121 atgctgtttc tcagaatcat attccaaata gtgctattga atcatgtgaa aatgatatta
    12181 aatacactaa acttgatttt gtgtttcctg tactgcatgg aaaaaatgga gaagatggaa
    12241 cagtgcaggg gttatttgaa ttggcaggta tacaggttat tggatgtggt actctttctt
    12301 cagcattatg tatggacaaa gacaaatcac ataaacttgt tcattcagaa ggtattaaag
    12361 taccaaaatc aatattacta aaatactcag ataaatttaa tgtggctgaa ctgtcaaaag
    12421 acttaactta tccattattt ataaaacctg tgcgtgcagg ttcatcattt ggtattagta
    12481 aaatttataa aaaagaagag ttacaagtag cagtagactt ggcttttgaa catgataatg
    12541 aagtaataat tgaggaaaat attgatggtt ttgaggtagg gtgtgcagtt ttaggaaaca
    12601 atgacttact tgttggaaga gtagatgaaa tagaattgtc aaaaggattt tttgattata
    12661 cagaaaaata tacattgaaa agttcaaaaa ttcatatgcc agctagaatt gatttggatt
    12721 gtgagaaaag aatacaagag acagcaaaaa ttatttatag aactcttgga tgttctggat
    12781 ttgctagagt agatatgttt ttaactccat ctggtgaaat tatttttaat gaagtcaata
    12841 caattcctgg ctttacttct catagtcgtt atccaaatat gatgaaaggt attggactgt
    12901 cttttgaaga aatattggac aagataattg aggtgtacat aaaatgatga ataatatttt
    12961 attagataaa aataaagaag ataaaatgac aaatactatc ttattgacta aagataaagt
    13021 ttatgatggg aatctaattc tagtaaatgc ttttttacca gttaaaacat ctgaagatat
    13081 agatttaatt cctgtagata caaggtttcc aagtattctt atgaaacgtg aagctacaaa
    13141 cattttacaa aatattttaa aaagtatatg tggaataaat gaaatagtac ctgtaagtgg
    13201 atatcgtact gcagaggaac aacaagatat atattcaagt tctctgcgtg acaatggaaa
    13261 agactttact aaaaagtttg tagcacttcc aaatcacagt gaacatcaga cagggcttgc
    13321 tattgattta gggttaaaga aagaagttat agattttatc tgccctgaat ttccttataa
    13381 tggtatttgt aatgagtttc gtaaggcagc actacattat ggttttatag agcgttatca
    13441 gtgtaaaaaa gaagaaataa caggaatatc tcaagaacca tggcattttc gttatgtggg
    13501 atatccgcat tctgagatta tcaataaaat gaatctcgct cttgaagaat atatagaata
    13561 tattaagaga ttttcgtata caaattgttt aaaaatagaa gataaagaaa aaattataga
    13621 aatcttttat gtgcctattc atactaaaga agaagtgaga atctctatac ctgaacaatg
    13681 tgtatatcaa atatcgggta acaataagga tggttttatt gtgacatttt ggagaaatag
    13741 aaaggactaa gaaatggagg aacaagaatg attaggaata aatcttatgc agggcttgat
    13801 tattttagga ttatagctgc attacttata attgcaattc atacatctcc attattatca
    13861 tatagtaaaa tggctgactt tgtgttaact agtataattg ctcgtattgc tgtgccattc
    13921 ttttttatgc tatcaggctt ttttctaatt tcaacatata attataattg taataaactg
    13981 aaatcttttc taaaaaatgt attatttatt tacgggatag ctattgtaat ttatattccc
    14041 ttaaatattt acactggata tttttccatg aataatttag tgcccaatat tattaaggac
    14101 attgtatttg atgggacttt atatcattta tggtatcttc ctgcatcaat tattggtgca
    14161 agtattgcat actttcttgt aaaaaaacta ggtttaaagt actcactagt aatcactatt
    14221 gttatttatg taattggtat gtttggtgat agttattatg gttttttaga aaaaatacca
    14281 ctattaaaga atttatatgg gtatattttt gaagtatcag attatacacg aaatggtata
    14341 ttttttgcac ctgtattttt catacttggt ggaataatat ctaataaatc taattatatg
    14401 tcaactaaaa atagcttgat tggatttctt atatcattct tacttatgtt atgtgaggga
    14461 atgtttttac ataaactagg ggtacagcgt catgatagca tgtatattat gttattgcca
    14521 tgtatgtatt tcttatttac agccttaaca tttttgaaag gttctcgagc taggttattg
    14581 cgaacatcat cattaattat ttatattata catcctatga tgattgttgt tattcgtatg
    14641 ttttctaaaa tattcggaat acaaactgtc ttggttgaca atagccttat tcattatata
    14701 gtggtaagta tagcatcttt tatagcttct ataattatta tattgattct taaacctcta
    14761 aaaaaagact tgaaaggaga acataaggta aataccaata gagcttggat tgaaattgat
    14821 ttaaataatt tggaacataa tgtaaagatg atacaaaaag ctatgccaga agattgtgaa
    14881 ttgatggcag ttgtaaaagc taatgcctat ggtcatagtg cttttgaagt agcgacttgt
    14941 gcaaaccaaa taggtgtaaa agcatttgca gtagcaacaa tagatgaggg gattgattta
    15001 cgtcgatatg gaatactagg agaaatattg attcttggat atactaaccc aataagggca
    15061 aaggaacttc ataaatacaa tcttacacag agtgttattg accttaatca tgctatctct
    15121 ttaaacaaac agaaatacaa cataaaatca cacattaaaa ttgatacagg tatgcacaga
    15181 ctgggttttg atgttaatga tgtgaggtca attttaaagg catttaaatt aaaatactta
    15241 gatatatgtg gcatatttac acacttgtgt gtttcagata gtctgaaaga tgaagatgta
    15301 gactttacaa ataaacaaat tgaatgtttt tataagttaa ttgattttct gttaaaaaaa
    15361 ggtataaaaa ttcctaaagt acatattcaa agtagttatg gtttttttaa ttatcccaat
    15421 cttaagtgta attatgtaag aattggaatt gcattatacg ggatattaag ttttccaaat
    15481 gatacgacta aattgcaact tgatttacga cctgttttat ccttaaaatc acaagtaatt
    15541 cttattagaa aaatacaaaa agatgatagt tttggatatg gaagagtctt tattgcaagc
    15601 agagatagcg taattgctat attatctatt ggctatgccg atggtatacc tagaaattta
    15661 tcctgtggaa aaagttacgt aataataaat ggttgtagag cagcaatagt gggtcgaatt
    15721 tgcatggacc aacttgctgt tgatattaca gatataccta atgtagaagt tggtaatacg
    15781 gcaataataa taggaagaga taatttatct gaattatctg tttctgaagt tgcaaataac
    15841 tcatgtagta tatcgaatga attattgagc agagtaggaa gaaggttaaa tgttataaag
    15901 aagtaactca attaaatagt aatatttgtt gtactgtgta atttatttta ctctgactag
    15961 acatttttag taatattatg tctaatatat attttttata acttacatga caatttaagt
    16021 tataaaaagg tgatgtttca gatttttatt ttgaaacatc accttttttg ttataatata
    16081 taataaaata attatacctt aactgtaaac ttatatagat tatttacttt ttaatattag
    16141 attcattttt tgagattggt atataataaa catatagagt atataataat agttaaagtt
    16201 aaaaaagggg aaaagcaata tgcagtatat acaagaacta cttgatttga ctcaaaatta
    16261 ttgggtgtta gccacaataa tcggcttatt aagtgcattt atagaaagct ttataccagc
    16321 cttaccactt gttgctatag ttacagcaaa tgctgcaata caaggattat ttattggatg
    16381 tttgttatca tggattggct caggacttgg gacaacttca ttattcttat taataagcag
    16441 atttaatgat agtaagcttt ttaataagtt gagaaatagt aaaactgaaa aagcaataag
    16501 ttggatggat aaacagggat ttaagttatt atttatagcg tatgcctgtc catttatgcc
    16561 cggttgttta gttactatcg catcagcttt tggtaaaaaa gatttaaaag attttgtgcc
    16621 agcaatgttg gcaggaaaat ttgtaatgtt tatagttata agttacgtgg caagtgatat
    16681 agaaggtttt ataacaagtc ctttaaagat agcatcattt atattattgg tgtttttgtc
    16741 atggaagatt ggaaatagag taaataaaaa tcttgaaaat cataattatg atttccatca
    16801 taaaaaacat cacaatgata aagatgataa aatgatttag cttgtacgaa ataaaacgta
    16861 tcttaaaatg atttagtact aaattatttt aagatacgtt ttattttttg tattaaggta
    16921 caaaattttt agttcttttg taaactaaaa tatataaaac ttatttatac ttagttcaaa
    16981 atattgttat ttattttttc tacaactata ataaaatata aaattgatat ccaatcaaaa
    17041 aaataactat gaaatttaat agcttagata tgaatttttc tgtttttgag ttagttttta
    17101 taggtagtaa agaaatgttt atagtaatgg gataaaacaa ttcaatacct tctttagtaa
    17161 taaggtcaag acacaagtga gatagatatc ctattataaa cccactggac aaacatgaga
    17221 aaactatatt attgttagtg aagattgtca gagatttaaa aaaactataa ataagtaata
    17281 aggcaattag actgtgagta aaccctctat gtctaaacct acttccaaat aatttgtata
    17341 ttaacataaa ttttttactt atgtaagaac ctctcatatc tatatctgga aataaagaac
    17401 ctaaatatgc aaaataaata taaattataa ttaatactat tttatatgca atatcgtatt
    17461 taactaggta cagattgttt ataaagggga gtatcaaaag tgcgaatata taacctcccc
    17521 tttcatgtgt ttcttttatc atataaaaat cctcctgaat cgtcactata aacattatat
    17581 tggtaaattt agactatctt ttattatatt atatagcaaa cataagtttt tttcaaaagg
    17641 attttaatat ttaattaatc ttgagatttt aaatttttgt aattttctaa agcaccattc
    17701 caatttacta cattaaacca gttgtcaatg tattcatttc ttctattttg atattttaaa
    17761 tagtaagcat gctcccaaac atcaagtcct attataggag ttaggttttt acttacaggg
    17821 ctatcctgat ttggagtagt cataatagat aatttcccat ctttagtagc tacaagccaa
    17881 gcccaaccag aaccaaagac atctaaagca gatttttgga actcttgctt aaatttttca
    17941 aaagaaccaa agtctctatc aatagcttct tttaaagatt cagaaggtat ggttttttct
    18001 ggcgtcatta tatcaaaaaa gaatttatga ttataagctc cacctgcatt atttcttaca
    18061 gttgtagcaa tatctttagg taaagaatct aaattttgca ataattcaca taaagaataa
    18121 ttataaagct caggatattt ttcaagagca gcatttaatt tatcaacata agcttgataa
    18181 tgcttatcat gatgcagttt cattgtttct ttatctatat aaggttcaag tgcatcatat
    18241 gcataaggta atggtttaac cttaaatttg ttattttcag gtgtaaaagc aaatgaagtt
    18301 atgcactgtg agattataaa taaagacata ataactggta ttaatatttt tttcttcata
    18361 aaaatcacct cctaatatag tatttctaga aataaaaaaa atatgaaata ttaacagata
    18421 atttataaga atttaatagc aatttcattt taaattgaat aagttttaaa gccaaagaca
    18481 aaactatgaa atatagaata agactaaagg acagaaagga attttgatta tatggacggc
    18541 aatatttcaa tttattataa aggtgatata tctaaaaaag aagatattaa atttaaaata
    18601 aaagaatctg taaataata
//
LOCUS       DACULQ010000071        18512 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.071, whole genome shotgun sequence.
ACCESSION   DACULQ010000071 DACULQ010000000
VERSION     DACULQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18512
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.071"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..230
                     /locus_tag="JA154_09655"
     CDS             <1..230
                     /locus_tag="JA154_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996128.1"
                     /translation="NEGNAKKLYDTQEEIYSGCKIIVGMEFETGYVFVEGSDELNDEI
                     TSFKGLDKYDINNYYLVANYIRCLKKYKLID"
     gene            485..1189
                     /locus_tag="JA154_09660"
     CDS             485..1189
                     /locus_tag="JA154_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HAT7996129.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            1186..1956
                     /locus_tag="JA154_09665"
     CDS             1186..1956
                     /locus_tag="JA154_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HAT7996130.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            1953..2729
                     /locus_tag="JA154_09670"
     CDS             1953..2729
                     /locus_tag="JA154_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HAT7996131.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLFMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            2784..3476
                     /locus_tag="JA154_09675"
     CDS             2784..3476
                     /locus_tag="JA154_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996132.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            3495..4919
                     /locus_tag="JA154_09680"
     CDS             3495..4919
                     /locus_tag="JA154_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996133.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(5158..7626)
                     /locus_tag="JA154_09685"
     CDS             complement(5158..7626)
                     /locus_tag="JA154_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996134.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGASENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTIAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTSISLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSILDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDNITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFATIFGSIAGYVSAIIINMATIEKLDFTNIPITSILQATIISILAC
                     IIATLIPLRKVKKMNIIDCIDVNL"
     gene            complement(7629..8297)
                     /locus_tag="JA154_09690"
     CDS             complement(7629..8297)
                     /locus_tag="JA154_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996135.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            complement(8583..9056)
                     /locus_tag="JA154_09695"
     CDS             complement(8583..9056)
                     /locus_tag="JA154_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase"
                     /protein_id="HAT7996136.1"
                     /translation="MLSFTFCSLPFWICYFWCGISDIFDGLIARKLRQQSGIGAKLDS
                     IADFIFSVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYRTFSSLHTLMNK
                     ITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELNRDIKSIFM"
     gene            complement(9327..10469)
                     /locus_tag="JA154_09700"
     CDS             complement(9327..10469)
                     /locus_tag="JA154_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996137.1"
                     /translation="MSLKKAFFVLTLSSLIIASTLTTFSYILLNNLYNSIQDKYLKTP
                     INTGFITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYKVKLKTP
                     IEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTMINDRKQ
                     LNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIEYYVENM
                     SNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLFFDENII
                     LRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRPFYRDKT
                     LNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            complement(10475..11149)
                     /locus_tag="JA154_09705"
     CDS             complement(10475..11149)
                     /locus_tag="JA154_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996138.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKGALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREKRKSNQTKIKFYNDLTIDYEKRLICIDNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            complement(11971..12798)
                     /locus_tag="JA154_09710"
     CDS             complement(11971..12798)
                     /locus_tag="JA154_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAT7996139.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGSKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            13180..13500
                     /locus_tag="JA154_09715"
     CDS             13180..13500
                     /locus_tag="JA154_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996140.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            complement(13633..14313)
                     /locus_tag="JA154_09720"
     CDS             complement(13633..14313)
                     /locus_tag="JA154_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAT7996141.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            14709..15761
                     /locus_tag="JA154_09725"
     CDS             14709..15761
                     /locus_tag="JA154_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAT7996142.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            15817..16224
                     /locus_tag="JA154_09730"
     CDS             15817..16224
                     /locus_tag="JA154_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAT7996143.1"
                     /translation="MAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSIDEF
                     SRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPMLLEL
                     FLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            16253..16726
                     /locus_tag="JA154_09735"
     CDS             16253..16726
                     /locus_tag="JA154_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIB"
                     /protein_id="HAT7996144.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSYNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            16773..17531
                     /locus_tag="JA154_09740"
     CDS             16773..17531
                     /locus_tag="JA154_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT7996145.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            17555..18394
                     /locus_tag="JA154_09745"
     CDS             17555..18394
                     /locus_tag="JA154_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAT7996146.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
BASE COUNT         6446 a         2431 c         3113 g         6522 t
ORIGIN      
        1 caaatgaagg aaatgcaaaa aaattgtacg atactcaaga ggaaatttat agtggctgta
       61 agattattgt aggtatggaa tttgaaacag gttatgtttt tgtagaaggt agtgatgagc
      121 ttaatgatga aataacttct tttaaaggat tagataaata tgatataaat aattattatt
      181 tagtagcaaa ctatattaga tgtctaaaga aatataaatt aatagattag tgtttgatta
      241 ataaattagt gtttgattta aggattatag agtaaatttg aagttagtgt gataatgaaa
      301 ctaagcttaa atctaatata actgtaaaac ataaacttaa atagagttgc caaaataaaa
      361 ttggtaactc tattttttat tttaaatttt attaaaatta gtaggtgtta tagaattgtt
      421 attttttcat gttagtattc tcttagtaac agatttaagg tattacaaga agggagaaat
      481 taatatgaaa gatttaattc ttgaaacaaa aaacttatca aaaagatatg gagaacaaat
      541 ggcagttaac aatgtttctc tacaaattga acgaaatagt atatatggac ttttaggacc
      601 aaatggagca ggaaagtcca caacacttaa aatgcttgtt ggtcttcttc gcccaacaaa
      661 tggtcgtatt atttttgata gaaagccttg gaaacgagaa tctcttgcaa aaataggttc
      721 tttaatagaa tcccctgcat tgtatggaaa ccttacagca gaggaaaatt tgttagtaca
      781 tacaaaaata ttgggaatac ctaaagataa aatttatgaa gtgttggaaa tagtaaattt
      841 gaaaaataca ggtaaaaaga gagcttcaca attttctatg ggtatgaaac aaaggttagg
      901 aatagctatt gcacttttga gtgacccaga gttgttaata ctagatgaac caaccaatgg
      961 tcttgacccc tttggcattc aagaattgcg tgaattgatt gcttcttttc caaaaaaagg
     1021 tataactgta attctttcta gccatattct atcagaggtt gctcaagttg tagataatat
     1081 aggaattata aatggaggac gactattatt tcaaggaatg cctgacccta atgaaaatct
     1141 agaagaattt tttacagatg ttattcttaa aggaggacaa aacagatgaa tgctttacaa
     1201 tcagaacttt taaagtataa aagaacattt atgggtaaac ttattgtttt tttcccagtg
     1261 tcttttgcag catatgcatt tataatgcaa tcaacactca tgcaaaatcc attatctcaa
     1321 acaacttctt gggcgtggca aagtctttta gcattaattt ttaactggtg gtcattttta
     1381 tttctgccta ttggatttgc attgtttgct actttggtag ctttccagga aaaaaaagct
     1441 ggaaattatc gtgctttacg tacacacaat gtgtctccta tgacactttg gattaataaa
     1501 gttatagcta tggcagtcta cagttttatt tcaacgctag ttttaatagt cgtaacaatt
     1561 ataactggtt taatattgaa agctggtcca gttccttttg gacagattat aggggcaagt
     1621 attgtatgct gggttgtttc tcttgctata cttccacttc agctatggct agctacatgg
     1681 aaaggtatgt tcctaagtat gggagttgga gctttaggta tgatatttgg tgtccttgct
     1741 gcgacaaaac cattttggat tgcagtacct tggagttggg cagttagaat ggtatgccca
     1801 atcatcaaca tacatcctaa cggtactata ttagaagctg gagacccact tttgaatact
     1861 tcagtgattc ctattgggat tgttgtttct ttggttgtgt ttatagtatt aacagcactt
     1921 actgcagctt ggttcaatag gagggatgac aaatgataaa gctattatca tcagaatggc
     1981 ttcgagcaaa gaggacagct gtccagtggc ttactctttt tatgcctatt attttttcat
     2041 tatgtgtggt tttttatctc acaactaaat ctggaagtac acaagaattt gcttttgaag
     2101 gattttttac agtatggact gtattcatca ttcctattgg agtaggtatt ttagcaggat
     2161 ttatagtgca ggaggaagag cttgctggaa attttaatgg ttttttatgt gtaggtattt
     2221 ctcgtgttag attatatctg ggaaaatttc tttttcttct gttttgccta actatatgca
     2281 catttattgc tacgttgata ctttgcatag gaatgagtat tgcagtgcca tctggagcaa
     2341 caatatggtt attcctgtca gcagcaggac ttgtggtgat tgggacactt ccactattag
     2401 ctattcatct ttgggtgagt tttgcatggg gtatgggtgc atctattggt attagtatcg
     2461 gtggaatttt gatggcagca atacttggat taactagcgt tggtgaaaaa gtatgggcat
     2521 ttgttccatg gacatggcca gtaaaactag gtatgcttcc aggaatttat tttattaaag
     2581 aagctggaac tatttcaact gaggcatttt actctaaagt gatgcaaaca gctagtatag
     2641 agcttatagt agtagcaata ggtttgatta tattcttgat aggaggcgtt atttggttta
     2701 aaatgtggga gggaaggaaa agttattagt agacgaatat tgttttaata ctgaccttcc
     2761 cataagagaa aggtggtgtt atattgagta aaatattgat tattgatgat gaaatggacc
     2821 tagtaatgct acttgaggat gaattgaagg caaaaggaca tgaagtgttg atagcttatg
     2881 atggtcaggc aggtatagag ttatcaaaac aggagcctga ccttattata ctggatatta
     2941 tgatgcctaa aatgaatgga tttgaggtat gtcaggcaat tagagatgat gttttatgcc
     3001 caattatatt tttaagtgca aagcaatcag aaaccgacaa aatcaaaggg ttgacctttg
     3061 gaggggatga ttatgtaaca aagccatttg gactgcgtga acttatggca aggattgaag
     3121 ctaatctgcg tagggaaaag cgttcacagt atatcaatga agagaacaag agatctactt
     3181 tgtattttgg gaagttatgc ttatatatga aggaacgtgc aattaaaatt gatggtaaga
     3241 atattgattt aacaaaaata gagtatgata ttgttgaatt actagcactc catgcaggac
     3301 aagttttctc aagagaacaa atctatgaaa aagtatgggg gtgtgattct gaaggtgatt
     3361 cctcaactgt tgttgaacga gtaaaaaaaa tacgagctaa gttttctgct gtgacaccag
     3421 aaaaagaata tatttccaca gtttggggta ttgggtataa gtggaataaa atgtaaagag
     3481 agagaggtta ttaaatgaaa aatcaatccc ttattaccca atttagacat acttttattt
     3541 ttattataat agccagtata gttgccacag tgattactta tgtatttgct ctctatttgt
     3601 atattcattc cttaaataag gatatctatc caccaaacta ttatgagcga caagttccta
     3661 gaattgaaaa atacatcaat gaaaaaaaca taactttgtt atcacaatcg aatgaagaag
     3721 gtttaaaaag aacgattcgt ggggatgata tgctatatca agttgtggat aataatggca
     3781 atattttata tggtactaac cccaaaaaac tatttaaaac aaaagaagaa ttatttaata
     3841 attttatcaa taaaactgtt cgtaaaggtg gttatatcca tactgttcca atcaaagggg
     3901 ataatggaaa gattgaggga gctgttattc tattctacca agtgaaaatc acctttgcaa
     3961 atattagagg gagatttgta tttgcagtta ttataatggc tctgttttct ccatttctat
     4021 atatcgtggg ttttactaga tggctttcta agcgctttgt aaagaatatc aatcaaccat
     4081 tacatctatt gattgatgcg tccaagaaga taaaagaaaa ggaccttgat tttgaaatcg
     4141 actattattc agataatgaa ctcggtaaac tttgtagtgc attttctgaa atgaaagacg
     4201 aattaaaagg ctctttatct gcacagtgga agatggagca ggaacgtgtt gaaatggtgg
     4261 aagctctagc acatgattta aaatctcctc tttctattat tttgggatat acagatgcac
     4321 ttattggtaa taatacagat gacaatgaaa aacttcatag gtatcttaca gtgataagag
     4381 aaaatacaga aaaaagtgct gctcttgtcc aaaagatgca atatacgtct gacttagaaa
     4441 agtcaaatat acaattaaat cttgttccta taaatttacc tgaatttttg aggcaaaagg
     4501 tacaagacta tgaattacag gctcatcaaa aggaagttga acttatattg aaaatgcagg
     4561 ggaatataca atccccaatt caaatcgacg tggataggtt gacacgaatt tttgataata
     4621 tcatatccaa cagtctgcaa tacactccat caggtggtaa tattagtatt actgtaaagg
     4681 atgaaaaaaa ttgtatttct tatgagattt gtgattctgg tagagggttt agctctaaag
     4741 atttaaaaaa ggctcttgat aagttttatc gaggagatga agctaggcag actaaaggag
     4801 gacattctgg tcttgggctt tacattgtaa aacaattagt ggaacagctt ggaggttctg
     4861 ttaaaataga aaattcaaaa tcaggtggag catgtgtgaa attttggcat agcatttaac
     4921 tatatatttt aaattaaagg ggagtgtctc aaagcgaaat ttttaattcg tgaggcactc
     4981 tttttttgtg aagttaaata tattttcatt atttcgacaa tgaaaaaagg cttatctcta
     5041 ttttgagaca ggcctttttt aaagcttata ttatagttaa aaagatttat tagttagatt
     5101 taaaagaaaa tagagacagt cattgataat ttaatttatt tattatctgt aaaatagtta
     5161 taaattgaca tctatacagt caattatatt catcttttta acttttctta acggaataag
     5221 agtagctata atacatgcaa gtatagaaat aatagtagct tgtaaaattg aagtaattgg
     5281 tatattagta aaatcaagct tttctatagt tgccatgttt ataataatag cactgacata
     5341 cccagcaatt gaaccaaata tagtagcaaa aattccataa taaacacctt cccataaaaa
     5401 catcttatat aagctactat tactcatacc aatagctctt ttaactccaa tctcattagt
     5461 cctagtattt atatttgtat gagttgtgtt aatgatgttt aaagtaccaa tcaatcccac
     5521 aaataatata actgcccatg ctagaaattc tatttgttta aaactctctt taagttgttt
     5581 atctgattca atatatgata tccaatgaga gccaggatta ttttcacaaa tctgttcaat
     5641 agacaatctt atttgttcta attttgattt atctttagca taaatattta tctggttgaa
     5701 cttgttttta tttgtcaagg tgttataaac agaatcatat acaataacat caacaccatt
     5761 tatccagcca ttaccttgta aaaagaacat tttatctact gtactatata tatctagttg
     5821 ttttttgtta attgtgatat tatctttagg ttttaaattt gtatgtctag tttttacacc
     5881 tggtgtagaa atagcaatcg gattttttat aaaacagata gaaccatttt taaagtcttc
     5941 tagtatagaa tctgtaatag aaggcattaa gtcttttaaa gtttgctcat caattccaat
     6001 gatttttaac atctcaccac tattttcaaa atttatatct gtatctattt cgccctgttt
     6061 gtattcagag tactttataa aggaagtgcg attgacgttt ttcattttat ctattttatc
     6121 tagtattgac ttatcaaaac cagatatttc atttgttaag gaaaaatctc cttcttttaa
     6181 attacttact gacctagata catctaatac attagagaac tcactcagtg caacgaatac
     6241 tgttatactc ataaataatg atagtatggt tattatagtt cttcctttat ttctttttaa
     6301 gtttagccag gctagatgtc tttcaaagtt ttttattgtt ttttctcgtt taatttttct
     6361 tttgattttg gtagtactgc cagccatagc aattttggga gagacatgtg aagcatatat
     6421 tgctgatggt aatgctgaaa taagtgaaaa aatcaaagat atactagtgc ttaatactaa
     6481 tgggaatata tatgctgttg tgtttttatg tataagctca gtaatttctt tattatcatt
     6541 tgctagaagt atatctggat ttaagatatt agtgaccatt ccagttatgc ctttagaaga
     6601 tataattcca attacagctc caataggtat ggcaatagta cataaaatta agatttgtaa
     6661 aataactatc ttatatatct ggtttggttc agctcctatt gctcttaaac atccatactc
     6721 cttaattttc tttgtaacag agatttttaa aatattgtag attacaagtc cagaagccag
     6781 taaaaccaat aaggcaacaa ataaagatac caatataatc atagatatac catcactatt
     6841 agaactattt tcatcttttt caaactgtat tttaagagca tttaagtaaa tccaattata
     6901 ttgaattgaa ttgtgagaaa gatttatttt tttagctaat tgatttacta tttcttgaaa
     6961 cttctcttgc tgttttattt taaaatccaa agaggataaa atatatctct taggtaatag
     7021 attttctgat gcacctttgc ctacaatacc gtttactata ccacttacat atcctgtata
     7081 atcatcctct aaaataccac tcaatttaaa gttagctgtg taattatatg gtggtattgt
     7141 atcatttaaa agtgaaatat ctaaattcat aggtatagtc actcctagtc ttggttttat
     7201 acccataagc tttagtgtgt tttcatctaa ggctatttcg tttttatctt taggcaaatt
     7261 tccagatttt aatttcatta atttggggta gttggaaagt cctgttttat catactcttt
     7321 tacaagtaca gagataccag aatcctttat tttactactg ccaagagtta ttgaagtacc
     7381 agcttgataa actctatcat ctttctttat gtcatcaact tgatttttag taagttggtg
     7441 aaaggataca tgtctatcac cgttaaaaga acgggcttgt tcaattctta aattttgtag
     7501 gataccaatt gactgtccaa ccacagttgt cataatagtt gatagtacta ttgctataat
     7561 gatgaatgta gtcataagtt tctggctttt taattctttt attgaaagtg aaaggtaatt
     7621 cttcatttct atttcacctc cataagaaca ccatcaataa ttgaaatttt tctatcagca
     7681 agttttgcaa ttctatcatc atgagtaatt accactaaag tctttccaaa ctgttttgaa
     7741 gtttttataa gaagattcat aacttcactg ccacttttac tgtccaagtt tcctgtaggc
     7801 tcatcagcaa atattatatc tggttttgca ataagtgctc tagcaattgc aactctttgt
     7861 tgttgaccac ctgaaagttc atggggaaga tgattgagtc tatctgcaat tccaagtaat
     7921 gatgagattt catttaaata ttctttatca ggttgttttt tatctagaag tacaggcatt
     7981 gaaatatttt cagatgctgt cagaacaggt aaaagattaa attgttgaaa gacgaatcca
     8041 aaggattttc ttctaagtgc agatagttct ttttcagtgt gtttatatat atctttatca
     8101 tcaagtaaca cttgtccaga ggtaggatta tctaatccac ttaaaatatg taaaagtgtg
     8161 ctttttccac ttccagaagg tccaattaca gaaacaaatt cacctgagtt tatttctaaa
     8221 tttacattat taagtgcgat aactttattg ttattagaac catatatttt tgataattgt
     8281 tttgctttta taatcataaa aaacactctc cttttctata taagaagagt gtaagtttta
     8341 aaactcaatt ttttcccaac ttttagctta aatctaaaaa gtcttcacta tttgcggata
     8401 attcgcagtg ctgataagca gtttcagtat catttttaat ttaaaatgta atttatgcac
     8461 taaaggcttt acaatacaac tctattgctt taaaactatt ttacacataa acttgtggat
     8521 aattttttat gttttacttc ctcattatta tataaattat ttttgtcaca aattctaagt
     8581 tttcacataa atatactttt tatatcacga tttaattctt ttgattttat cataattgct
     8641 atttcttcaa aagaagaaag aaaagccacg atacatatca taattcctgc aacatcaaga
     8701 ccaaagcaaa tatagagtaa tggaaacgca aagatagaaa ttccagtgat tttattcatc
     8761 aaagtatgaa gcgatgaaaa tgtgcgatac ttaaaaaaac caatactgta actaatcaac
     8821 cgtaacaatg ctattgcaac aatacaccac catagataca tgggaagagc aatatttttt
     8881 ccgacagtaa ccaaaattgt aaccgaaaaa atgaaatcag caatactatc taactttgca
     8941 cctattccac tttgttgtct caactttcgg gcaataagac catcaaatat atcactaatt
     9001 ccacaccaaa aataacatat ccagaaaggc agtgaacaga atgtaaaact aagcattagc
     9061 aatgcaaaca caatacgaga gaatgtaata ttattagcaa aacttttaat ataatcacct
     9121 ccaaaattct aatttcatac tattctacat atttaaatat atatttctat tattgtttca
     9181 aatgcctttt gccaaaatat tataattgaa gcaactctta caatttctac aaaatagtat
     9241 atatattggt tataaattga aatacatttt ataaattcta aactaacaaa ttttatataa
     9301 tagcagtgaa agcttacaaa atcattttaa tttcttgttg agaatttagc agtaatttta
     9361 gcactgttag tagaattatt ttcaatagaa atagagccac catgtttttc acataatatt
     9421 tttgagatat aaagacccat accaaagtgt gcattgctgt catttaaagt cttatctcta
     9481 tagaaaggtc ttaaagctaa ttttagagat tccttactaa atcctatacc atcatcttca
     9541 attacaaagg taaggagttc ttgttcaaga tatataagta tggatacatt atttctagca
     9601 tatctaaaag cattcgatat tatattttcg acgactctaa gaatgatatt ttcatcaaaa
     9661 aatagattta tattatttat ttgactttta agtgtaaaag attttccttc ttgtttagca
     9721 atgatagata tattttcatc taagttttct ataaacttgt ttatattcga catagatttt
     9781 gaaacaacta aatcttctaa tcgttgagca ttactcatat tttctacata gtattcaatt
     9841 ctgttaatat gctcagacat aagttgtgtg gttgataaaa tcttctcatc actaagctta
     9901 cctgttggta tgtattttgt taaatagttt gagtaacctt ttagaactgt taggggattt
     9961 cttaaatcat gagaaaaagc agcattaagt tgttttctat cattaatcat agtccacatc
    10021 tttatgttgt ttttatttag ttcagacctc attttttcaa aagcattaca tagattacct
    10081 aattcatcat tattattata ctctaagcaa aagtctaaat tgttgttgga tatttccatt
    10141 gcacctttat tgagtatttc tatgggtgtt tttaatttta ccttgtaaaa aattatatca
    10201 gctaataata aaagtcctat aaaaaatata agtggaagta caagctgaag tatgttgatt
    10261 atattaagta acgttttatc ataatcacta aattggttca cattgttttg aactatggtt
    10321 ataaaacccg tatttatagg tgtttttaaa tacttatctt gtatagaatt atatagatta
    10381 ttaagcaaaa tgtatgagaa agtagtaagt gttgatgcta taattagtga agataaagta
    10441 agaacaaaga aagctttttt taatgacata ttatttattt tatccattta taaccaactc
    10501 cccagactgt ttctatataa ttttccagag tatgttttga tagtttgatt cgtatctttc
    10561 taatgtgttc cattataaca tcactatttc cattgctatt gtatccccag actctatcat
    10621 agattttttc tttatcaaat acttgtccag gattcataga gagaagttct actatatcaa
    10681 attccttttt tgaaagtgaa atctgattat tatctataca aattaacctt ttttcgtaat
    10741 ctatcgttaa atcattataa aatttaattt tagtttgatt tgacttcctt ttttcacgtc
    10801 ttaaatgagc agatactcta gctccaagtt cgtctaaatc aaaaggtttt acaatataat
    10861 catcagcacc agcacgaaaa ccatttatct tgtcagtatt ttcaattcta gctgtaagaa
    10921 atagaatagg gcatgataca taatctctaa ttctattaca aacttcaatt ccatctattt
    10981 caggcatgtt tatatcaagt agaataatat ctggattttt ggatattttt tttaatgcgc
    11041 cttttccact ttgagcagtg taaacctcat aaccattcat ttcaaagtaa tcttttaaga
    11101 gtgttattat accttcctca tcatctacga cgagtatttt attgttcata agaatgctcc
    11161 cttctaaatt aagcttgtat agtatgtata ctttagtata aaaatctcaa taaaatctca
    11221 aattcttagt tatatttcaa aacttaatca ttaaagttag tatattttta tatattaaat
    11281 aaatcaagga tgaaattatt agaaatttta atagaatata tatttgatat ttaatactta
    11341 ttggaatgca aaaacacagc tgatgaaatc ttataattta agtttaaaat ggataaatag
    11401 aaaggaagaa ttttaattaa aaaggtagat taattaaaaa gtatataaat tataaaaatg
    11461 aattaatcta cattaattag ttgatatatc aattattatt gtttttttca gttgcttaaa
    11521 atatactcga tatatcaacg aatgttgttt tttaattaaa agaaatttta ggaaagataa
    11581 tatcaactag gagagataat atcaacattg aagaaggtag agcaagattt ttacataatt
    11641 gtgaacattg tctggaatgt gtgcattggt gttttaagca agtaattcaa tagaaagatg
    11701 tgactaaaaa tagaaagaaa tactataatc caaatgtaaa gctagaagac ataataagta
    11761 aaaaaagtaa agatatttaa taaaagaaat aactaatagt gattaaatta gtaaatgctg
    11821 ataattagca taatcattag taattattag tattatttaa ttaaattaat attagtatta
    11881 tttttatgaa attaatttta aatcaaatta atgagaaccc cataaatttt tttaacactt
    11941 aatttatgat ttatggggtt atgttctgtt ttatttcaaa gtattaagtt catataaatc
    12001 agttcttctg tgcttaagaa gtggtaactg ttgtcttatg tcaggaatta aatctctatc
    12061 aatgtcagca taaataatac attctttttc atcagcttga gcaattattc ttccccaagg
    12121 gtctgaaatt cttgaatttc caaaagctat gtaggaagca ttcatatttc tagcaggtgc
    12181 agcaccaaca tagaatatct gattatctaa agcacgcatt cttatagata actcccaatg
    12241 ggcgggacca gtagtcatat taaatgcagc aggcaagata acgatttcag cacctttaag
    12301 agccattaaa cgtgataact cagggaaacg aatatcataa caaatagcta taccaacttt
    12361 tccatattcg gtatctatta cagtaacatc atttccagct gtaagagtat ctgattcttt
    12421 aaaactaact tttcctgtaa cttcaatgtc aaataagtgc attttacgat gttttccaat
    12481 tagagctcca tttttatcaa atacatagca agtattatag atttttccat cttctaactc
    12541 aggaatacta ccagaaacta gataaattcc atgtttctca gctaatttac tcaatattgt
    12601 tgttgtttca cctggatatt cttcagcaaa tttaggaaaa tatttatttt catatggaca
    12661 gttaaacatt tctggcaaaa ctacaatttt agatccattg atggcagctt ctgttaccat
    12721 atctacagca tgattgatat tttcaatttt attttctgta gtaatcatct gacatactgc
    12781 aattttatag aaattcatag aaattactcc tttcaatatt tttatgttat tataacatat
    12841 taaataaaaa ttagatattc ttagattgac ccaaatgggt atggagagaa gtgaataaga
    12901 atattaaaag gtatataaaa gttattttta ttatatatgg aacccatata ttgacttttt
    12961 ttatatatgt tgatagtatt attatagata aaatagaata attaatagag ttttataggg
    13021 ttccgcaatt attaatgatt ggtctggtcc gagataaaac ccacaacata gtttttgttg
    13081 tgaacacgga aggacaaaaa cctgggagat attttataat atttctcagg ttattttatt
    13141 ggaaaaataa gagacatatt tgaaaagagg gatgaattta tgaaatggtt atttttgttt
    13201 gttgcaggtt tgtttgaagt gttttgggct tttcaactaa aaaattcaca tggattttct
    13261 aaattaactc caagtatact tactgttgtt ggaatgattg taagttttta ttttttgtca
    13321 ttagcactaa aacatcttcc acttggaact tcatatgcaa tatggactgg aattggtacc
    13381 gtcggtacag ctattgtagg catggctatt ttaaatgagc ctattagtat atcaagaatt
    13441 gcatgtatag gttttattgt ggtgggaatt gtagggttaa agctattatc tactaattag
    13501 agtatattaa tttaaataag ctatgaaatt aaaggcgaga aaatgtttaa aaataatcaa
    13561 catttaaaaa gtctttactt catagcttat cttatttata agtttattat atttgtaaat
    13621 tatatgtata tactatttat ctaataaaag agcagttaaa tccatagggt caactattac
    13681 accatcttta taaacacgca tatttccaga tgctatttca tctattaaga ttacttcatc
    13741 attgtagaat ccaaattcaa atttaatatc ataaagctca agaccttttt cagctatagt
    13801 atcgcgaact atgttagaaa ttttttgagt catgcttttc attgaatcaa attgagcttg
    13861 agtcataatg tttaaagctt ctaaaccttc agatgttata agagggtcac aacggtcatc
    13921 atcttttaaa gttgcttcta cgtaacaatc tagtttagca ccttcttcaa cacatgaacc
    13981 ataacgacgt aagaaactac caacagctct ataacgacaa ataacttcta atccattacc
    14041 aaaaactttt gctggtagaa cttccatagt tgcttcttct aaattagcac ttacataatg
    14101 agttttaatt ccagcattgt ttagaatttc aaagaactta acagatgtct ctaaattagc
    14161 tctaccaatt ccatctatac ttagacctac actattttcg cctgggtcaa atactccatc
    14221 tttaccagtt acatcgtctt taaatttaag taagtaattt ccattatcta attcaaaaac
    14281 atctttagtc tttccttgat acactttttt cataaacaac ctccaaaaag ttaaattatt
    14341 atattgaata aggcattagt gtcaaaaaag ttctgatatt cgcatgaaaa tttatattga
    14401 taccttatat ttaatattat atcacaaata gaagggaaat ttccgaacaa ttttataaaa
    14461 ataacaaaaa aagtacattt tataaaactt ataataagag aactaatata aaatgttagt
    14521 gtcgtttata ttgacacaca taagttttaa tataagaaac ttttgacaca ctttgacaca
    14581 catttaatga taattttata aaatgcatga atacaatgct tacatatatt ggcatgaaag
    14641 ttgctttaga tatttaacat ataaacaata gggatatatt ctcaaataaa aagtaggggt
    14701 ggttaatagt gaagtcagtt agattctatg gtataagaga cacaagagtg gaggatgtgg
    14761 atgtaccaaa aatattagaa aaagatgatg taataattaa agtaaaagta gctggaatat
    14821 gtggttctga tatatcaaaa tattcaaaga caggtccaca tatggttgga gaaattttag
    14881 ggcatgaatt ttctggagaa gtggcacagg ttggtaaaga agtaaggagt tttaaaatag
    14941 gagatagagt tgctgtttgt ccagctatgc catgttttga atgtgatgaa tgtaaaaaag
    15001 gcttgtattc aagatgtaat aatgtagcaa taataggaaa taaagaactt ggaggttgtt
    15061 ttgcagagta tacaaaggtg aaagagagaa atttaataaa aataccagat gagataagtt
    15121 atgagactgc cgctgcgcta gaaccagtat gtatagcagg acatggtctt tttaggtcag
    15181 aagctaaagt tggagatact gttgtagtac ttggtacagg tccaatagga ttattttcaa
    15241 tacaatgggc aaaaatattt ggttctacaa aaataatagc tgtggatgta tttgatgaga
    15301 agttagactt ggcaaaagag ttaggggcag atatttgtat aaatgcaaaa gaaaagaata
    15361 ttgtagaaga aataaaaagg ttaactgatg gagatggtgc agatattgtt attgaatcgg
    15421 ctggtactcc actgacatgt ggtcaagtac tgttgctagc taaaaaggga ggtacagtat
    15481 tgtatgcagg tgtaccatat ggggatgtag ctttgactag agagcaattt gaaaagatag
    15541 taagaagtga attaactgta aagggaactt ggtttggaaa ctcctttcca ttcccaggaa
    15601 aagaatggag tgctggattg tatcatatgc aaaagggtga catgaatgtt gaaaaattag
    15661 taacacatag aataaattta gaagaagtac cagcttattt tgaaaaagta tacaaaaggg
    15721 atattttctt tggtaagata atgattaata tagataatta gattagagta ctaaataata
    15781 atccatataa aaaaggttaa gaaaggtatg attcatatgg caggaataat tatagccaca
    15841 catggaaata tggctaaagc aatgttagaa agtgctgaat taatagtagg caaacaggaa
    15901 aatgtagaaa ctttaggatt aaaccatggt gacagcatag atgagtttag tagaaagtta
    15961 gaagatggaa ttgagaaata taaggatgaa ggggttttag tattgcttga tttttatggc
    16021 ggaactccat ttaatacatc tgctattata ataaataaat ttgctaaggt atgtgagtta
    16081 gaatgtttga caggtgtaaa tttacctatg cttttagagt tatttttaaa cagagatact
    16141 atgaagttaa aagacttgaa gaatttatgt gaggaagttg ggatatcagg aatcaaagat
    16201 gttaaaactg ttttaaagtt gtaaaataaa ttaaaaatct ggaggaataa ttatgagtaa
    16261 tatagcatta acaagaattg atgatagatt aatacatggg caagttatta ctgcttggtg
    16321 taagattaca tcagcaaaaa ggatagtaat cgtggatgat ttagtagtaa aggacccatt
    16381 tatagttcaa gttttacaaa tggcagctcc atcaactgtt aaggttgaag tacatgatgt
    16441 agaatcaggt gctgaagtct taaaatcata taatggagat gaaaatttaa tagtattggt
    16501 caaataccct aaaactgtct taggtttagt aaatagcggg gtagatttaa aggaattgaa
    16561 tgttggaggt atgggagcag ggcttggtag aaaaagtttt tataaaaata tatctgtgtc
    16621 agatgaagaa aaagaaatat ttagagagct aatatcaaaa gatgttaaat gctttataca
    16681 aatagtacca gatgcaaaga aaattgatgt aggaacatta ttgtaaagaa gactatcttg
    16741 taaatcttta aaagaaaata gggaggaaga atatgcacat ttcactaatt caagcagtat
    16801 taatagcaat tttttattat ctttcttgga gtccgtggtt gacttatgtt ggatttttta
    16861 catggaatag acctctattg gctggatttg taacgggaat tatattagga gaccctgttc
    16921 aaggagcaat tataggagca ggtataaata tgatatatct aggttttata tcagcagggg
    16981 gagctcagat gggggaccca gcttttgcag gatatgtagg tactgcttta gctatagcat
    17041 ctaagctaga tgtaagtact gcaatggcaa tagctgtacc attgggaact gttgcaactg
    17101 tactttggat tggaaagatg acagtaaatt ctttttttgc acattgggct gatagagagg
    17161 ttcaaaaagg gaatgttgat agagtagcat ttataaatat agtaccacca cagatactgt
    17221 tatttgcaat gagttttata ccagcattat tagttgttta ttttggtcca aatgcgatag
    17281 atggtatgtt ggaagtaatg aatgataatg tgttacatgt atttaatgta attggggcta
    17341 tgttgccagc acttgggatt gctatgaatt taaaattgat tggaaacaag tttactatgc
    17401 cctttttcat attaggaata ctaatggctg tttattttaa agtagatata atagtaatat
    17461 ctgttattgg agtaatactt gctcttacaa ttacttcaat taagtacgga aaagatagtg
    17521 ctacatctta aatacatgtg agggggaaaa tattatgtca gatataaaaa atgaagtatc
    17581 taagaaaacg ttaagcaaga aagatgtaat aaaatcatgg ttaagatggt ttttctttgc
    17641 acaatctaat tacaattatg aaagattgca atcaacagct ttttcacatt caatgttgcc
    17701 agtacttaaa aaattatatc cagataagga agagttaaaa caagaagtgg agactcatct
    17761 agcatttttt aacacagaac ctatatgtgg atgtgttata catggaataa caatagctat
    17821 ggaagaagaa aaagctaatg gagcagatga aataagtgga gatggtatga atgctatcaa
    17881 aacaggcttg atgggtccac tagcgggaat tggcgatact ttaactcaag gagttataac
    17941 tcctatagtg ttggctgttt gtattggttt aactgaggga ggagcatcaa ttgcaggacc
    18001 tatattattt gtaatagctc aatatataat aatgacttca atttcttttg gaatgtggac
    18061 aaatggatat aagtatggga aaaaagccgt tgaaagtata cttcaaggtg gaatagtaaa
    18121 taaggttata gaaggggctt ctatactagg taccttagtc atggggggac ttgtaggtag
    18181 gtttattaat ttatctacac caataagtta tactagtggt gattttaaat ttagtcttca
    18241 aacggattta ctcgataaga tatttcctgg gttaattcct ttggtactta ctttattagt
    18301 actctttgct ttaaaaaaag gtctttctcc aataaaaata atgtttatac tgatagtagt
    18361 gggagctatt acaggtattt taggactatt ttaaataaat atatattggt gtaagttagg
    18421 attctatatc tattgattat atttgtagga tgttggaaaa tttatattaa aaagtttggc
    18481 aattagggta attttataat agattaaatt tt
//
LOCUS       DACULQ010000072        17567 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.072, whole genome shotgun sequence.
ACCESSION   DACULQ010000072 DACULQ010000000
VERSION     DACULQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.072"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            26..361
                     /locus_tag="JA154_09750"
     CDS             26..361
                     /locus_tag="JA154_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7996147.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            498..1775
                     /locus_tag="JA154_09755"
     CDS             498..1775
                     /locus_tag="JA154_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein CcsB"
                     /protein_id="HAT7996148.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKEQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1896..3284
                     /locus_tag="JA154_09760"
     CDS             1896..3284
                     /locus_tag="JA154_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089526.1"
                     /note="Involved in disulfide oxidoreductase activity and
                     electron transport; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HAT7996149.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3779..4201
                     /locus_tag="JA154_09765"
     CDS             3779..4201
                     /locus_tag="JA154_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996150.1"
                     /translation="MIISIEVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNL
                     IGKFFNKYNAQNLKFYLDAPVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLD
                     RVVSSDAVIVDKCISYFNVARGIIEEYIKECNIINLNK"
     gene            4337..5188
                     /locus_tag="JA154_09770"
     CDS             4337..5188
                     /locus_tag="JA154_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996151.1"
                     /translation="MKLTKLNIKKYRAPIYKYALANINLRSAKSTNSSIITVIPQGAK
                     MEVLDEEDDWIKVMYNSQEGYVYKELVSVSEYAWSNLNLREDKSTTSNIITVIPEKSR
                     VEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNENNIKST
                     SDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRKPSFGTD
                     EYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWIYDNIPD
                     TTTVIIH"
     gene            complement(5343..6488)
                     /locus_tag="JA154_09775"
     CDS             complement(5343..6488)
                     /locus_tag="JA154_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAT7996152.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(6821..7597)
                     /locus_tag="JA154_09780"
     CDS             complement(6821..7597)
                     /locus_tag="JA154_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAT7996153.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8179..9153
                     /locus_tag="JA154_09785"
     CDS             8179..9153
                     /locus_tag="JA154_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HAT7996154.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9251..9556
                     /locus_tag="JA154_09790"
     CDS             9251..9556
                     /locus_tag="JA154_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT7996155.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9712..10872
                     /locus_tag="JA154_09795"
     CDS             9712..10872
                     /locus_tag="JA154_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAT7996156.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            10922..11824
                     /locus_tag="JA154_09800"
     CDS             10922..11824
                     /locus_tag="JA154_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT7996157.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            11904..12797
                     /locus_tag="JA154_09805"
     CDS             11904..12797
                     /locus_tag="JA154_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HAT7996158.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      12850..13031
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13250..14389
                     /locus_tag="JA154_09810"
     CDS             13250..14389
                     /locus_tag="JA154_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7996159.1"
                     /translation="MKEMKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGK
                     KLELKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLI
                     ASTTQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVD
                     LNPDLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKE
                     DMATSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGI
                     NACASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKN
                     GNLWVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14461..15486
                     /locus_tag="JA154_09815"
     CDS             14461..15486
                     /locus_tag="JA154_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAT7996160.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            15489..16685
                     /locus_tag="JA154_09820"
     CDS             15489..16685
                     /locus_tag="JA154_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996161.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFNLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(16813..17538)
                     /locus_tag="JA154_09825"
     CDS             complement(16813..17538)
                     /locus_tag="JA154_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAT7996162.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         6728 a         1968 c         3077 g         5794 t
ORIGIN      
        1 taaataaaga aaagaggaga ataaaatgta caacatagat gatatgatat ataattgtcc
       61 agtagaagct ttatcaaata ttttaggtaa aaaatgggtg gctataatta tatggaaaat
      121 acaagaagat aaaaaacgat ttggagagct acaaagagct ataccagact gtagtaagaa
      181 aatgttagtt caacaactag attttttaat tgaacagggg attatagtca accaaaaaaa
      241 gttggttaat aatgttgtag agtcaacata ttttttaagt gaatcaggat tacgattgct
      301 tcctgtcatg gaaaaaatga ttatgtggag caataaaaat ttaaattgtg ataaatacta
      361 aaatttactc tgtaagtaat gaaaatttaa ttaagctatg agtagaaaaa tgtattaaaa
      421 ttacataatt ttttagttag attttaattt agaaatggga ttgtaatatt ggaaataaaa
      481 taaggaggaa ttttaatttg caaaatgtaa atttattttt agtatttata gaaggtatag
      541 tatcattctt ttcaccatgt atactgccaa ttttgcctat ttatttgagt atattgtcaa
      601 atagtagtgt agaaaattta aaagaaggaa agacgagttt tataggaagt tctttattta
      661 aaaatactat attttttgct cttggaatct caacaacatt ctttatattg ggttcatcag
      721 taaaagtatt gagtatgttt tttaatgaaa ataaagattt aataatgttt attggtggga
      781 ttataattat tatcatggga ttattttaca tgggaataat taaatcttca atattgaata
      841 gagaaaaaag atttaatgtt aagtttaaag aaatgaaagc aataacagct tttatattag
      901 gttttacgtt tagttttgga tggacacctt gtattggtcc aatattggca tctgtattag
      961 tcatggtttc aagttcaagt aatcatctta gtgccaatct tttaatagca gtatatacta
     1021 ttggttttat tttgccattc ataattacag ctatgtttta cagtaaacta tttaaaacta
     1081 tagataagat aaaatctaac atggaaatta ttaaaaaaat aggtgggatt atacttatag
     1141 tatcaggaat attaatgatg gtaaatggat ttggaagtat aagcaaacac tttaatacgt
     1201 cacaaaatag taaaattgaa agcaagcaag aagaaaataa gcgagaaaat tctactgata
     1261 aagaagaaaa ttctgatggt aatgatagtc aaaaagactc taataatgac aataatgata
     1321 aagggtctaa tgatgaagat agaataaagt ctattgattt tactttaaca gaccagtatg
     1381 gtaaaacaca taaattaagt gattatgaag gcaaagttgt attcttaaat ttctgggcta
     1441 catggtgtcc tccatgtaaa gaagaaatgc cttatataga acaactgtat aaagactata
     1501 ataaaaataa tgatgatgta gtaattttag gagtagcttc acctaattta ggtagagaag
     1561 gttcaaggga acatgtagtt aacttcttaa aagaacaagg ttatacattc cctgttgtac
     1621 ttgatgaaga tggagcatta gcatatcaat atggaattaa tgcttttcca actacattta
     1681 taattgacaa ggaaggatat gtaactcagt atattccagg agctatggat aaggcaacta
     1741 tggcatcatt tattgaaaat cagagaaata aataaaagta tttaagtata agtttataaa
     1801 tcaatttaag gttacaaaaa agtgcctaat tgtaccacct aaataatctt gttatagtaa
     1861 aagtataaac aatacaagaa agggttgaat tttatatgaa aaaaacattt gatgctataa
     1921 taataggatt tggaaaaggt ggaaaaacat tagcagggga tttggcaaac aggggattaa
     1981 aagtagcttt aatagaaaag tcaaacaaga tgtatggtgg aacatgtgta aatgtggcat
     2041 gtataccaac aaaaagctta gaaaattcag caaacagcgt aaaaacaaag aatataaatt
     2101 cttgggatga agtacaagct gaatatgaga aagctataga taaaaaagaa actttaatta
     2161 caaaactcag agaagctaac tataataaat taaattcaaa tgagaatgta actatattca
     2221 caggaatggg aactttcatt gatgaaaaaa cagttcaagt caaaactgaa aatgaaattt
     2281 atgagttagt agcagataat atatttatta atactggttc aagaccattt atacctaata
     2341 ttaagggaat tgaaaataag aatatagtat atgatagtga gtctcttatg aatctaagag
     2401 ttttacctaa aaaaatgaca attataggag ctggatttat aggtttagaa tttgctggta
     2461 tttatagttc atttggagca gaagttacaa tattaaactc taataatggc attttaccaa
     2521 atgaagatgt tgaggattca gaagaaataa taaaattact tgcaaagaga aatgtaaaaa
     2581 ttgttaataa tgcaaatata aaagagatta aagaagtttc tgaattagca atagtagaat
     2641 atgaagtaga tggtaaatct aaagaattaa caagtaatat gatacttgta gctacaggaa
     2701 gaaaagcaaa cactgaaggg ctagggctag aaaatgcagg tatagaatta aatgaaagag
     2761 gatttattaa agtctcagaa actcttaaga ctaataaaga gcatatatgg gctataggag
     2821 atataaatgg aggtccacaa tttacttata tttcattgga tgattatcgc atagttatta
     2881 atcagttatt tggagataag actagaacaa ctaatgatag aaagaatatc ccaaattcta
     2941 tatttataag tccagcattt tcaagagttg gtcttaatgt taagcaagct aaagaaaagg
     3001 gatatgaagt attagtggca aagatgccag tagaagcaat tccaagagct aaacagattg
     3061 gcaaggcaga tgggtttata aaaatagtga tagataaaaa atccaataag atacttggag
     3121 ctagtatgat atgtgaaaat tcaagtgaga ttatccatct tattcaatta gctgtagatt
     3181 tagaagtaga gtatacatat ttaagagacc gtgtttatgc acatccaact atgacagaag
     3241 ctcttaatga tattctatct cctaatatga taaaagaagt ataacataag tttaatatat
     3301 aaatctaaaa ttatcattat aagtagattt tagaaagagg ctatctcaaa aaatagagat
     3361 aagtctcttt tttactgttt aaataataaa aatatatttt aatggtatta aaaattatct
     3421 taaaaggtga aataatctgt atttaatgta gtacagttat atctttaaag caaggtggtg
     3481 aaatgatata aataagatat ctaaaagagg gtttgatgat agtgataaaa gatggttttc
     3541 aagtaaagaa ttaacaagac ttataaaggc taaagaagaa atagaatggt tattaaatag
     3601 ggattacaaa atagacccag tcgttacttt tgttggtgat agatatcagt tttctataag
     3661 acaaagagat gcattaaaaa gggcagtctg tactgatgaa aaaaatatta taagacaatc
     3721 aaagaggctt tctctagata aaatcaaaga agataccgta aatatagatg gctttaattt
     3781 gataatatcc atagaggtag gattatctgg tggtactcta gtaataggaa gtgatggcaa
     3841 tataagagat ttagcaggtc ttaggggaac ctataagatt atagataaaa cagaagaagc
     3901 tttgaatctg ataggtaaat tttttaataa atacaatgca cagaatctta aattttattt
     3961 agatgctcca gtttctaatt caggaaattt aaagtatagg atattagagc atgccaaaac
     4021 ttggggaatt gaaactgaag tagaattagt aaaaaatgca gatgttgtat tggaaaaatt
     4081 agatagagta gttagtagtg atgcagtcat agttgacaag tgtataagtt attttaatgt
     4141 ggctagaggt ataattgaag agtatattaa agaatgtaat atcataaatt taaataaata
     4201 gataaatata ggtgagaata tataagtata tgtagcaata ttcttagtaa tacaagtaaa
     4261 gtttgaaaat aggagaacga atacatatcg aatttcataa aatgttgtaa aaagacatat
     4321 gagaggatgg atatgtgtga aattaactaa actaaatatt aaaaaatata gagcacctat
     4381 atataaatat gctttagcaa atataaattt gaggtctgct aaatctacca attcaagtat
     4441 cataacagta atacctcagg gagccaaaat ggaagtattg gatgaggaag atgactggat
     4501 taaggtgatg tacaattctc aagaaggata tgtgtataag gagttggtat cagtaagcga
     4561 atatgcttgg agtaacttaa acttaagaga agataaatct acaacttcca atataattac
     4621 agtaattcct gaaaaatcta gggttgaagt ccttcaagta gatggagatt ggagtaaggt
     4681 tgtatatgat gataaaatag gatatgtttt taattatttt ttatcaattg atgggaataa
     4741 accaaatgaa ttagattata agtattttta tactgacatg attaaatttg ttaacgaaaa
     4801 taatattaaa agtactagcg attatctaat tgtaacagac cttagaaata aatatactta
     4861 tatttttaag aaagataatg gaggatgggg acaactttac aagtggcaat gtactatagg
     4921 caagcctgag actccaacta taacaggtat attttatata agtggaagaa aaccttcttt
     4981 tggaacggat gagtattcag ttaagtatgc tactagaatt aaaggtggat attactatca
     5041 ttctgtacta tatgattcaa caggaagtta tataatagat ggcagacttg gtgaagctct
     5101 tagtcatgga tgtataagat taagtacgga aaatgcaaag tggatatatg ataatatacc
     5161 agatacaaca acagttataa ttcattaaga aaaattttga gttttttatt gggataatga
     5221 ttcttaaatt aataggatta tatattaact tctcataaag agtactattt aaaaagtgta
     5281 ataagagagt gctattaaca aaacaaagat attttgaggc actctcttat aaatatacat
     5341 aattaagata ttttcataaa caattctata atcttatctt tatcaaagaa aacaggatta
     5401 gcaccagcac aagcatcttt catagagttt tctgcaagca attcaaaatt aggattatca
     5461 acacctacat cttttaaaga ttttgggatt ccaacctctt cagaaagagc ttttatctta
     5521 tctattacaa agtcaacaca ctctttatct gatttattat ttacatccat tccaataact
     5581 tcagctattg gtcggaattt ggcaggggct gatttagcat tttcctcctc aactattgga
     5641 agtagcatgg cattacatac tccatgaggt aaatcatata aacctcctaa ttgatgagcc
     5701 atagcatgta catttccaag acctgcatta ctaaaggcaa ttccatttaa aaaacaagca
     5761 taacacattt gttctctagc ttctatgtca ttaccatttt taacagctct tggaaggtat
     5821 ttaaatatct gctttatagc atataaggca gttgaatctg taacatcata agcaccatta
     5881 gctactacag cttctatagc atgagttagt gcatccatac cagttgctgc tgttaaatta
     5941 gatggtttgc taatcattaa ttctgggtca tttactgtca ttgtggctag tgaattggta
     6001 tcaaccatta tcatctttac atgtctgtct tcatcagtta taacatagtt tattgtaact
     6061 tcagcagatg taccagcagt agtagttata gctatgatag gaagacattt tttagaagtt
     6121 ttgttgatac cttcatagtc tctaatatct cctccattag tggctaatat agagatagct
     6181 ttaccacagt cttgtggaga accacctcct atagttataa cgaagtcgca attttctgat
     6241 tttaatattt taagtccttt attcacatta gttacagttg gattttgttt aacttcatca
     6301 taaacaacaa agtctatatc tatctgagat aaaatatctg ttactttttt tacagtacca
     6361 tttgaaacta gaaatttatc actaacaata aatgcttttt tagttcctaa tgatttaatt
     6421 gaatctttag cttcattaag acatcctttc ccaagtaaat ttattggtgg tacataataa
     6481 atgctcataa ctaaaatctc cttttatttt cattgatttt tatgtaaaaa tatatatcta
     6541 acaattttca ctaatttact cataatatat cgtatcttag attgagatag ggttgctggt
     6601 tcttatataa gaacttaatt cgtaattacg attatgatta gaatttaaca tagtattttt
     6661 atcatgtcaa taaaaatata aaaaatatta taaaaaaaac aaagttactg tagttaaatc
     6721 agcaactttg tttttatagt ataaatcaaa taaatacatt agtttaaaac aaaattttta
     6781 attattatgt tttgatatct tccatataca gaatattaat ctaatttatt acaatgtctt
     6841 cattacgttc ttccatcatt gattgaatat ttttctgagc atttaagagt aattttttta
     6901 tcagttctat tttttcgtca ttagctctaa ataaaggcag tgttacacta acagcagctc
     6961 ttatacatcc attttttctt attggtacgg caagacattt taggtgttcc attgattcct
     7021 cacactctga agctatttgt gttctttgaa cttcaagaag ctggtcgtat aaaacatcaa
     7081 aatctgtaat tgatttttct gtaaaacctt ttagaccatc tggatataag cttattagtt
     7141 tttccttgtc aaaatcacat aaaagagctt ttccaagacc tgtacaatta gcaggtattt
     7201 tttttccaac ataagaaata agtctaatcg gttctggaga atctactttt ccaatataaa
     7261 gaacttcatt attgtcaagt ataccaagct gacaagtttc agaacaggaa ttaactatat
     7321 tagtcatttc gtcaattata tagttaaaaa tagttctttt ttcaaaaaat gaagacccta
     7381 atgtgtaaga acgtatccct atagaatatt tggatgtgtt tttatcaata gtaatgtatt
     7441 ttctagcatt aagtgtatga actataggaa agatgctact ttttggagca ttgataacat
     7501 ctgctatttc tgtaagagta tatccatttt cggattctgc taatgcttct aaaatatcta
     7561 atatcctagc tgttgggcga tgattatctg aagtcataaa tttccccctc tttttattaa
     7621 tttaaagtat tagaatatgt tgattttagc acactatacg atattttaca atgtagaaaa
     7681 taaatatgta aatttatata taaataagct cttgatttag atacagatag atattttttt
     7741 agactgtaca ttaaggaata actaatttcc aaaatatacc taaagaaagt aagtgatatt
     7801 tagttatata atttaagtaa atatatgcaa tagttatttt tatatgtaaa tatcaaaata
     7861 ttatgaactt atattttatc tattttttac actgtataaa aactttatag ttttgaatac
     7921 tatataattt taaatacgct gtatttttgc atataaggaa gtcatttgat aatatacaag
     7981 gtattatcag aaagtttgaa aaaattttga caaaaattta ttttttttat tgactcataa
     8041 ataggaaaat gctatcatta attcaaagat tcgtaattaa aaacttagtt cttaaatacg
     8101 aaccgaaacg gccgttagaa ttaaagctaa aaacttaata agagtaatta aatatcatgt
     8161 tttaggagga ttaactttat gataatggat tttattaaaa aaattcctgc aggtatgatg
     8221 atagtgccaa tgtttatagc agctttcatt aatacctttt gcccagatat tgtacaaata
     8281 ggttcgttta cgacagcagt attttcaagt gttgggtctg caactataat tggggttcaa
     8341 ctggtatgct tgggaactca gcttcaattt agagaaatgc caaaagtttt aaagagaggt
     8401 ggtatacttc ttggttctaa atttgctata ggtgctttga taggtatact tataggaaag
     8461 ctatttggaa tggatggtgt ccttggtctg acaacattgg ctgttataag tgcagttaca
     8521 aatagtaatg gtagtatata tctttcattg atgacttcat atggagatga aacagattgt
     8581 gcaactatga gtttacttac tttaaatgat ggaccatttt ttacattaat agctcttggg
     8641 acttcaggtc ttgcaaatgt accattgttg tcattattgg ctgcaatagt gccaatactt
     8701 gttggtatga taataggaaa ttttgacaaa aaaatgaaag agtttttaga gccagggtgt
     8761 aatttactcg taccttttgt agggtttgca cttggtagta gcataaatct tgcaagtata
     8821 ctaaaaggag gtttatcagg agtattactt ggattaatat ctgtatttgt aggaggtata
     8881 tttatagtat tctgtgataa atttatagga aaacgtccag gatatgcagg atgggctgta
     8941 gctacaactg ctggaaatgc aattgctgta cctgctgctg ttgcattagt tgacccatca
     9001 tggcaaccat atgttgcaac agctacaaca caagtggcag ccgcagtagt tgtcactgca
     9061 atattagtac ctcttataac tagttggtgg gctaagaagt atggatgtcc taagttccca
     9121 aaacaaaatg aggatggtac aataacagct taatttatat tacagtgagt tgttggtaaa
     9181 ataataaatt ctttaattag attttaggat attaataaaa ttatattatt tataaaggag
     9241 agcaagaata atgataaatg cagtgataat tgatgaaaaa gataatgttg cagtagctat
     9301 tgaagcaata gctaaaggta gtgaaattag ttttaaatta aaagacaaaa cagtaaaaac
     9361 gattacagca ctagatgata taactattta tcataaattg gctataaaag atatggccaa
     9421 aggtgataaa gttactaaat atggtgaaca tataggagaa gcaaatcagg aaataaaaag
     9481 tggccaacat gtacatattc ataatgttag aagtgttaga gaggatttgg ataaacaagt
     9541 tatgactaat atataaaata atatttatag agttataaaa gttgtattca ttgcttttag
     9601 tgtcaaatag tttttaaata ggtttttagc tgtttgatta gtgagtgtaa taaaaattat
     9661 aatatataaa aattatagaa agtatataga ctattttagg aggaaatcag tatgaaattt
     9721 tatggatata aaagaccaga tggaagagtt ggtataagaa accacatatt aatattgcct
     9781 acgagtgtgt gtgcatcaga tacaactaga ataatagcat cacaagttca aggggcagtt
     9841 acctttaata atcagaatgg atgttcacaa gtacattcag accaacaatt aactatggat
     9901 gttatggctg gttatgcagc aaatccaaat gtatatggtg taatagttgt atctttggga
     9961 tgcgaaaatt gccaaaatga tttggtagtt gatgcaataa aagagagaac aaataagcca
    10021 ataaaaactc ttgtaataca agaagaacat ggaactttaa aaactataga aaaagctgtt
    10081 cgttatgcta gagaaatggc acaagaagct tctttgctta gaaaagaaga atttccaata
    10141 tctgaactta tactaggaac tgaatgtggt gggtctgacc caacaagtgg attagcagca
    10201 aatccattaa taggtgaatt aagtgataaa cttgtcgatt taggagcaac atcaatttta
    10261 agtgaaacta ctgaatttat aggtgcagag catatactag ctaggagagc agtaaatgaa
    10321 gaagtaaaag aaaaaatatt acatatagta catagatatg aaaattcatt aaaattggtt
    10381 ggagaagaag ttagagaagg taatccatct cctggaaata tagctggtgg tttaacaagt
    10441 ttagaagaaa aatcacttgg ttgtatacat aaaggtggtc atagacaaat aagtgaagta
    10501 tatgattatg ccaaacaaat tgataagaaa ggtcttgtta taatggatac ccctggaaat
    10561 gatgcttcat ctgttgctgg tatggtagca ggaggagcac aaatagtggt atttagtaca
    10621 ggaagaggaa caccatcagg aaatccaata gcaccagtaa taaaaataac tggaaataaa
    10681 ataacttttg caaatatgga agataatatg gattttgatg ctagtccagt tatctatggt
    10741 ccacaaacaa tggaagaatt aacagatgat ttgttaaata tggttgtaga tgtagcaaat
    10801 ggaaaacaat caaaagcaga gtcccttgga tatactgaaa tggcaatagc tagagtatgc
    10861 aactatgtat aatctttaag taagaaaata aatacataga attttaataa agggtgagat
    10921 tatgaagttt gtaagtttta ctgaaggaga aaatgaatgt aaaagaactg gagttttttc
    10981 taaagatgaa ttatttatta tagatgttaa ctctttaaat ctcagtagaa attttaagga
    11041 tttaaatgaa ttaattcaaa atgtatctag tgaagatatt attaagttga aaactattat
    11101 aaatgaaagt acattctata attatgatgt ttttaaaaga gagagatgta cggtacatgt
    11161 tcctatagaa aaaccaatac atgatatctt atgtgtggga gtgaactata aagaccatct
    11221 tgaagaaact caaactcact ttgatgatag ttttgaagaa ccacaaaaaa cggtttattt
    11281 tacaaaacgt gtgacaaagg caataggtcc agaagatgag atagatggtc attttgagat
    11341 aaataatcaa ctagactatg aagttgaact tggagtagta ataggtaaaa caggtgttaa
    11401 tattagaaaa gaagaggtag aagattatat ctttggttac actgttataa atgatatttc
    11461 tgctagaaag ttgcaaaaag aacatgttca atggtttaga ggaaaaggat tagatacatt
    11521 tacctcttta ggtccatgta ttttacataa aagtgcagtt ccttttccta taaaacttaa
    11581 agtatgtagt agggtaaatg gtgaagaaag acaaagttct aatacaggat tattcatatc
    11641 agacgtttca gacatagttt cagaactttc aaaaggtatg accttagagg ctggagacat
    11701 tatagctact ggaacacctt ctggagtagg gatgggattt tcacctcctc gatatatgag
    11761 tagtggagat gtagttgaat gtgagattga gagtattgga attttaaaaa attacattaa
    11821 gtaactgttt taatataatt aatattaaag ctaaatctga tgtattaatg aacatattaa
    11881 aataaatttt aggagtttaa attatgaaag cagaattttt gacaccagtt gttacaatat
    11941 ttgatgaaga aggaaattta gataaagaag gtaataaaaa agtgtatgac catcttatag
    12001 atggaggtgt agatggacta gtcataatgg gaagtactgg agagttcttt aatatgtcta
    12061 tggatatgca aaaagaactt atagatttag taactagtca tgtaaataag agagtaaaag
    12121 tgtttatagg tacaagtcgt atgtgtataa atgaatctat agaactttca aactatgcac
    12181 ataatagtgg agcagatggt gttatgataa taagccctta ttatttctct cttgatgatg
    12241 atagtattga gttattttat agtgaaatag ctaagaatac agaggctgaa atatatttgt
    12301 acaattatcc agatagaact ggttatgatt taagtccaga acttacatta agacttgcaa
    12361 gaaaacataa aaacatagtt ggatataagg atacagtaac tttgatggga cataccagag
    12421 atttaataaa tactatgaaa atagagttcc cagaatttaa ggtttatagt ggttatgatg
    12481 aaaactttat acataatcta atgtctgggg gagcaggttg tatagggggg ttatcaaatt
    12541 taattccaga agtttgctcc aaatgggctg aagcttacaa taatagagat atggagaagg
    12601 ttatagaaat tcaaaaatat attaataaag caatggattt ttacttaata accaatccat
    12661 ttataccagc aatgaaaaaa gctatgaata tcagagggtt acaaataagt gattatatga
    12721 ctgctccatt tgtaaaacca aatgaaaaac aagtatgtga aattaaaaaa ttgatgaatg
    12781 aattaaatat atgttaaatc aaccttgcaa agaggtcaac aataaattac aattatctta
    12841 ataaaaatta aattaaaata atactagggt actatattag tttaataggg aaagtgatga
    12901 aaattcacta cagcccccgc tactgtatac ggatacgaaa tcaaaatcca ctgaaattta
    12961 taaaaatttt gggaagggtg atggaggaaa agccgtgagt caggagacct gcccagtatt
    13021 atataagttc aatttagggt ggagattgta atttataata aaatgaagta tcaattcgtt
    13081 tttgtaaagt tgatatatga acttctcctt attaaattag gagaagtttt ttattttatt
    13141 aaaaggttat acttattaaa gatgacttta ggataacttt aataagtata ctttaaaaag
    13201 catatattat taaagttttt tagccactca taaaaaataa gatattggag tgaaagagat
    13261 gaaatttaaa tcaaatgttt taaagcagtt ttttttaatt gctatggtaa tttttatggt
    13321 aggaggtctt atagggtgta gtaaaagtga caaagagaat aaacaagcta gtactggtaa
    13381 gaaattagaa ttaaaatatg ctagtggatt tagtgttgaa gatttagaag atggaattaa
    13441 aaaagtaact gatggagata atcgagaact gattttaatc ccaaagaaga ttaaaattcc
    13501 tgaaaaatat aaaaatgcta atatagttag aacgcctgtg gacaatgtat taatagcatc
    13561 tacaacacag gcttgttctc tgagaacaat agatgaacta gatagtataa aagccgtaac
    13621 aacagaagag cagtactgga ctattaaaga aattaaagat ttaatgaaag atggaaaaat
    13681 acattacgtt ggttcaaatt tagcaccaga ttatgaaaaa atagtagact taaatcctga
    13741 cttaactata gtgagtagtg gtttatcaga ggctgataca aaatttattg acaaattaaa
    13801 agaattaggg cttaattatg ttgtagacaa tgagtataaa gagcaaaatc catttggaag
    13861 aatggagtgg acaagtttaa tagctgcatt ttatgataaa gaagatatgg ctactagtga
    13921 actaaataaa actgtacaaa aggtagaaga agtaagtaaa aaaataaaaa acaaagctaa
    13981 acctaaagtt gtatgggcat ctgtatatga aggaagtgct tatatagctc ctaaagattc
    14041 atatgtggat aatatgataa aaatggctgg tggaataaat gcatgtgctt caatagattc
    14101 aaatgaaggg cgagtaagta ttgaacaatt gcatgaagtt gcaaaagatg cagatatatt
    14161 tgtatactca tcaagttcag attatgcacc aaatttagat tctataaaat ctagtgcacc
    14221 tgtattggct gatttggatg ttgtaaaaaa cgggaaccta tgggtgtttg ctccagatta
    14281 ttatcaatca gtagataaga cggacgaatt aatagtagat ttagttgaga tgttccaccc
    14341 aggtactaca ggtgaaaaaa tcaaacatta tataaattat gataaatagt acagagagga
    14401 cacaaaaaga taggttttat agattttagt taatatagct ttgctaagag gagtatacaa
    14461 atggatttta tcaataaaat gagaataagg aatacaacaa tatttatagt attaattata
    14521 agtatgatac ttactttttt agtgtgtgta gcatttggct ctgtaaaaat tgaatttaaa
    14581 gaagtaatag atgttgtatt aagaggtatg gataatacag aacattataa aataatatac
    14641 aatataagaa tccctagagt acttgcaacc atggtaggtg gagcatgcct agcagtatca
    14701 ggtattttat tacagatatt ttttaaaaac ccaatagttg aaccgtatat attaggagta
    14761 tcttcaggtt ctactttatc agtagctctc atgattttgg gaggatacac attgggattt
    14821 gagagtgtaa attcaatgag tatatttgta gcagctttta taggagcttt aattgtaatg
    14881 tctatagtca ttttatttgc aaataaggta aagaatataa caactctttt aataatagga
    14941 attatgaccg gatatgtatg tagtggtata acaaatgtgt tacaatcatt tgcaaatagt
    15001 gataaattaa aagaatttac tatgtggtct atgggaagtt tttctggatt tacatgggaa
    15061 aaggtagaaa tacttataat agttggtata ttaggtcttt tgatgactgt attcataata
    15121 aaacctttaa atgctttttt actaggagaa gagtatgcaa aaagtattgg cataaatata
    15181 agatttttta gagttttgat agtatttata tctagtatat tagcagcaat agtatcagca
    15241 tttgcaggac ctatagggtt tattggactt gcagtgccac aaatttcaaa aatgatattt
    15301 aaaacatcag ataatagagt tttgatacca gtagtaatat ttttaggcag tatagttaca
    15361 gctctttgtg atttaatatc aagagtaatt ttatcaccag tagaattacc aataagtacg
    15421 ataacatctt tattaggagc tccaattgta gtaatgttat tggtgaaaag gaaggatagg
    15481 gcttagacat gttgaaaact aataatttaa gtgtaggata caataacaaa gttgttataa
    15541 gtaatataaa tgtagaagta aaaaatggag aaatactatg tcttttggga agtaatggag
    15601 ctggaaaaac taccctatta aggtccttat ctaaacttat atcaccaata aaaggtgaaa
    15661 tatatttgaa tggtgtaaac ataaactgta tatcaagaaa agcattatca aaaaaaatgg
    15721 ctttagtact tacaaataga ttattaggtg atttaatgac tgtacaagat atagtcaata
    15781 taggaaggta tccatataca ggattttttg ggagtctatc taagaaagat ttaataatgg
    15841 tagatgaagc attggagtct gtagatgcct tacatttaaa gaaaagatat tttgatgaac
    15901 taagtgatgg agaaaaacaa aaggtgttgg ttgcaagggc tcttgttcaa gaaccagaaa
    15961 taattatatt agatgagcct acaactcact tggatataaa acatagattg gaactcataa
    16021 acatactgaa aaaattaagt aaagaaaagt caatatcagt aatactttca ttgcatgaaa
    16081 tagacatagc attaaaaagt tgtgataaag ttgctcttat aaaaaataat aaagtcatag
    16141 cttatggtca gcctgaagat gtagtagatg aggatattat aaattcgctt tatgaattgg
    16201 atgataaaaa ctttaatagt cttttaggtt cagtagaaat tagtaataaa tctaaaaatg
    16261 aagtttttat aattggtggt gggggtaaag caacaccaat ttatagagct tttactaaaa
    16321 aaggtatagg tctgtattct ggaataatac atgaaaatga tattgattat gaaattggta
    16381 gaactatggg gataaagatg tttacagaaa atccatttga gcctataagt gatgagagtt
    16441 tcaatttggc tattagaaat ttaaatgatt ccaagattat aatagataca ggatttagtg
    16501 taggagagac aaataagagg aatatagata ttataaaaga ggctttaaaa ttggataaaa
    16561 aagtttattc ttttagaaat agagatgaga gtaagaagta ttatgacagt ttggatagta
    16621 agatagaaca tatagataaa gtttctcaaa taatcaatag tgcagatata aataatttat
    16681 tataatttat gaaataaaat gtacttatgt gctatagaaa aaagagtata gattataatt
    16741 tttttgaaat tacaatctat actcttttgt tattttgttt atgtatttat atatttataa
    16801 aactttagca ttttatagat aaccaatagc ttcctctcta gttaaaaaaa agtgaatccc
    16861 tgtagtcgat tctttccatc tattttcatt aaaatcttca gctacaatcg tttctcctac
    16921 tctatatata aaattttcat cagcatatgc acgagcactt ttaaaagatt ctttaccatc
    16981 tatactagta attgacagta cttttgcctt atcacatcta caagcgtttg atgtagcaga
    17041 cactcttttt gcgtcgctgg gtattaataa ttgtaccatt ctaaaattaa aacatttttt
    17101 atagcctata aatgctcctg tttcaggaca atacaattta aaaaaacggg tactttcatc
    17161 tgatattact ccatttaaag tagcatgttc taaatttgaa ttttctaaat tagctcctct
    17221 aatatctgag ttagataagt cacaaaatct aaaacttgca ccacttaagt ctgcattttt
    17281 taatatagaa ttttttaatg aacaatccga aagataagaa ttattaaaaa tagagttttc
    17341 taaatctgct ttttctaaat ttaaattgat aaggtcagac cacgaaaagt caatattact
    17401 taagcagtat cctcttaagt ccatattttc taatcttaat tcttttaaat ctgccttttc
    17461 tccagcatta ttgtttttaa gccataaata atgtttctca agtgtttttt ctaattcttg
    17521 tttagaaata gttttcatta aaatcacctt cataaattta tttttat
//
LOCUS       DACULQ010000073        17489 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.073, whole genome shotgun sequence.
ACCESSION   DACULQ010000073 DACULQ010000000
VERSION     DACULQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.073"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(427..2322)
                     /locus_tag="JA154_09830"
     CDS             complement(427..2322)
                     /locus_tag="JA154_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HAT7996163.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(2358..3236)
                     /locus_tag="JA154_09835"
     CDS             complement(2358..3236)
                     /locus_tag="JA154_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAT7996164.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(3941..5374)
                     /locus_tag="JA154_09840"
     CDS             complement(3941..5374)
                     /locus_tag="JA154_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAT7996165.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KITDGVIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPKGDESTPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     NRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAMPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     HTLKLWERKNYNLDITEEDKKILKEGIVDFIGCSYYLTTVVTADKTMKTTGNDSAGKA
                     DVVENPYLKISDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDVLKSDNTVDD
                     DYRIEYLASHIREMKNAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFDWYKNVIKFNGEIL"
     gene            complement(5397..7238)
                     /locus_tag="JA154_09845"
     CDS             complement(5397..7238)
                     /locus_tag="JA154_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit EIIBCA"
                     /protein_id="HAT7996166.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETIIKKAVLQGQNTEEQGEKVSIVSKIFE
                     SISGAFSPLIPALAGAGMIKALLTILTTMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLSANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDIISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     NGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYTTHMNAGDKVKKGDRIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            complement(7364..8197)
                     /locus_tag="JA154_09850"
     CDS             complement(7364..8197)
                     /locus_tag="JA154_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAT7996167.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNNEESLSELHSLLDRIPLKDVELVSEIIQMGREELGDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYNFSKKAVEYLRKKLDKNIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENTNVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            complement(8602..8703)
                     /locus_tag="JA154_09855"
                     /pseudo
     CDS             complement(8602..8703)
                     /locus_tag="JA154_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(9241..9441)
                     /locus_tag="JA154_09860"
     CDS             complement(9241..9441)
                     /locus_tag="JA154_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7996168.1"
                     /translation="MKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNPSLKL
                     AMDIAKVFDTTVENLFEFIEEE"
     gene            complement(9454..9870)
                     /locus_tag="JA154_09865"
     CDS             complement(9454..9870)
                     /locus_tag="JA154_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996169.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(9940..10353)
                     /locus_tag="JA154_09870"
     CDS             complement(9940..10353)
                     /locus_tag="JA154_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996170.1"
                     /translation="MSKSELGVKANLVSELMMLSYFFIDLLLQKFIFKGEGIWFTFII
                     LLAFWVCNLMYNKKYNDLVDEASKLMLLKVDQIASKIMLYSMFVIAVLFSVNHTRALD
                     ISNLDIGIMLLAVLFIQSLSKLILYIYFDKKGICN"
     gene            complement(11187..11816)
                     /locus_tag="JA154_09875"
     CDS             complement(11187..11816)
                     /locus_tag="JA154_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAT7996171.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDVRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(11895..12857)
                     /locus_tag="JA154_09880"
     CDS             complement(11895..12857)
                     /locus_tag="JA154_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAT7996172.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQNQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(13619..17317)
                     /gene="cbpA"
                     /locus_tag="JA154_09885"
     CDS             complement(13619..17317)
                     /gene="cbpA"
                     /locus_tag="JA154_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HAT7996173.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAEGDTNAGNAGGDANAGNAGGDANAGNAGGDANAGNAGGDANAGNAGGD
                     ANAGNAEEDSNAGNAEGDANAGNAEEDANAGNAEEDSNAGNAEEDMNALVVEEATAED
                     PTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAEL
                     IFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLT
                     IDDSVVNRDIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYY
                     SAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTIT
                     AKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFG
                     DGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLG
                     GAVAYGDLEKKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDDQG
                     VLYLWFSDQFKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQFLKQYEEFTIDDT
                     PPYLIIKRYDGLPFEEDVEKLFLDWILKDGGIPIITPKGEKLTDKKFMTISSQRLNEE
                     TMEPDTNLGSDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHRAYYKAEIIPADTE
                     TSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVE
                     LKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLK
                     LDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGN
                     GDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPN
                     LNKPDPDNGGNSKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGN
                     GGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNGGNSKPDI
                     DTDGDGKPNINIDIDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDIDGDGKPDI
                     NIDIDGDGKPDINIDTDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNVQTGDQS
                     NIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT         5555 a         3247 c         1822 g         6865 t
ORIGIN      
        1 ttttatatta aaagtttcta aatatttctc aatcatcttc aaatcttttt gtaaagtttt
       61 atcagataca tttaatttat ctttaaagta ttttataggt ctatatttat tttcgtttag
      121 tatcaattta agaaattcaa tttgcctcga cacaagcata cttagccctc cctacctatg
      181 tttatatatt tctatgttta tattaagtac tattttaaca tatttactaa ctatgtaaaa
      241 ttactaagat acttctgtat cataaaagaa tttatgatac accttactaa attgatataa
      301 tagataatat aatttcaaaa taaaaattaa ggatatttag aagtagttaa ctgattctaa
      361 atatccttat tattattcta tatacttgtt gttattccat taattatttt tactattatt
      421 tataatttaa gcaccaagta attcaacttc actattttca ctccttatag taggctttgt
      481 tacccctata atcaatgctg ttattactgt accaatcaat atacaagcta tatatgcaac
      541 tggtgcacta catgcaaatg gtattacaaa tattccccca tgtggtgcca ttaaagttat
      601 atctaaactc atacatattg ccccacctat acaagaacca attatatttg ctggaatcat
      661 tcttaatggg tctgcagctg caaacggtat tgcgccttct gttatgaaag atgcccccat
      721 tatagcagtt ggtagagatg actttctttc ttctttagta aacttatttt tgaatattaa
      781 catagctaat gatattgcta atggaggtac cattcctcct accattaatg ctgctgatgg
      841 ttcaaatata ttacttgaaa acatagccaa tccaaaagct gaagctgttt tattaatagg
      901 tcctcccata tctgatgcca tcatccctgc taatattgca cctaataata ctttattagt
      961 tccagacata ctttgtaacc aagattgtaa agcttcattt aaccatgatg ctggcgtatt
     1021 aactacaaag aacattatag ctccactcgt aaatgcaatc aaaagtggac atatcaatac
     1081 agcatttact ccttttaata catctggtaa attttctgtt atcttcatta ctcctaaagc
     1141 tatatatcct gttgcaaaac cagctaccat accacctata aaacctgaac ctccaataac
     1201 tgccataaaa ccagctatca tagcaggagc aaatccttgt cttcctgcta ttgatttagc
     1261 tataaaacca gcaagtatcg gtataaataa cttaaatgct gaatcgcctc caagttgtcc
     1321 aaagaatcca gctatagggt tgtatgtttc atgagttggg tctgatgcaa atataccaaa
     1381 cataaaagct atggctatac caattccacc tatcactaca aatggaatca tttcagatac
     1441 accactcatt aagtgcttat aaacaccctt tttctctgac ccttgaagca tttcttttga
     1501 atcttctaaa gtctttccac ctggtttaat ttctttagcc attccatttt cagcgtttat
     1561 aattaaattt ttagctaatg aaacattctt agcatctact actttctttt gtagcacttt
     1621 ctttcctata aatctactca gttgaacttc tttatcacaa gctattatta cgtattcagc
     1681 tctttttata tcttctttag tcaacttatt ttctacacct atagctccat gagtttctat
     1741 tttaacttca tatccaagtt cttgacctgc ttcctctaat gctttttttg ccataaaagt
     1801 atgagctata cctgttggac atcctgttat cccaactata agacctttat tttgattatt
     1861 actaaaatct cctgattctg cagctttatt attaaatata cttaatattt cgtatggagt
     1921 ttctgcactt agtagatgct taagaaaatt ttcttccatt agatatgtag ctagtttaga
     1981 tataatctta agatgttcat ctgaacctga ttctggaact gctatcataa aaaatgacct
     2041 aagtttttga ccatcattat ctgtaatctc tctatctgtt cttaaataca tcattacagg
     2101 ttgatttact acatttgact tagcatgagg tatagctatt aaattaccca tattagttga
     2161 tgattctttt tcgcgtttat taatatcagc tataaacttt tctttattat ttatataatt
     2221 tccatggtac aaatgatttg ccatataatt aattacatct ttttttgttt gtaattgttt
     2281 atctagtaca atacaatcgc ttcgtagtat tttacctatc ataaattctc ccctcctttt
     2341 aatttgttac ctcataatta atttttattt ttataaaagc actttaactt atctatagca
     2401 ttgaatagat tcattttaaa ctctataact tctcttgcag attttataca tggtggatat
     2461 atagtatctg gctcatataa gcttgggttt tgttctaata gttctctgca cttcttataa
     2521 tatgccgact taacatcact cgaaatattt attttacaaa ctcctagttt aactgcttgt
     2581 gctatttctt catctggatt tgatgaacca ccatgaagaa ctaaaggtat atctactact
     2641 tctcttattt cttttaataa atctagttta agctctggtt taaaaccttt tggatatata
     2701 ccatgtgctg taccaattgc aatagctagt gtttctactc ctgttttttc aacaaaatcc
     2761 ttagctaatt ttgaatctgt atatattatt tcatcagtac caccttcact tgatgttcct
     2821 gttgttccta ttgttccaag ttctccttct acggacacat ttaatccttt tgctatttct
     2881 actatttccc tagatatagc tacattctct tcatatggta aatgtgagcc atcaatcatt
     2941 actgatgtaa atccacatct tattgccctt aatatttgtt ctttattttc cccatgatct
     3001 aagtgtatta ccataggtac tttactctta tttgcttctt ctatgcaact agctacaaag
     3061 ctatctccta gaaaacttag ctcatttggg tgtattgcaa gtattactgg tgcatttttt
     3121 tcgttagcac tctgtactac tgctttaagt atttgcccac ttcctatatt aaatgctggt
     3181 acagcaaatt gattttcttg tgccactttt agcatttcct tcatatttat taacatattg
     3241 tacctcccga tattttaaat tttaggttga atttgcttct tgttaatttt gttgttgttt
     3301 atatttaaat aatatcaggg taatgaagat cagaaaataa caatcgtttt ccttagtgaa
     3361 aaggaacttt tattatacac tctaagttcc ttttcactaa ataattttta gaagtctgca
     3421 tcatagttaa ttatatttct catatacatc ttctcttttt aagtgtttta tgctttagcc
     3481 ttcttaactc tttccaataa acaattagat agtttagcta taaatttaaa gatattcgta
     3541 tcgtagttag cactatattg gaatcaaaaa aattaaaaag atactagata gcagatacca
     3601 attattaata caccataagt catagatact tctatcttaa attcttgcat attagttaca
     3661 atagaaatta aataggacta ttataaaaat cgtaaggtaa gactgacatt tattctccaa
     3721 ctatacaaat gaatactaat atagctcctt ctaattaaat catactatat aaatcatata
     3781 tatcccaatt tttaacctta cctctttata tttacttttt aataaaaaaa gatattgact
     3841 aaatagtcaa tatcttttag tggatttaaa tacccacgat tttaatctaa tttttaatgg
     3901 tttccttaac ataatcttat aaatattaaa atttatctac ctataaaatt tctccattaa
     3961 attttattac atttttatac caatcaaaag atttcttttt atatctttta agagtcccac
     4021 ttccatcatt atttttatct acatatataa atccatatct ttttttcatc tctcctgttg
     4081 taaatgatac tggatctata catccccaaa cagtataccc tataacatca acaccatcaa
     4141 tttcaatggc atttttcatt tcccttatat gactagccaa atactctatt ctataatcat
     4201 catctacagt attatctgat tttaatacat cttctgctcc aaatccattt tcaacaatga
     4261 atataggaag ttcatactta tcatatagtt gattaaggta aaaccttaga cctacagggt
     4321 ctatattcca tccccaatca gaaattttta agtatggatt ttcaactaca tcagcctttc
     4381 ctgccgagtc attacctgtt gttttcatag ttttatcagc tgttactaca gtagttaaat
     4441 aataactaca tccaataaaa tcaactattc cttcttttag gattttttta tcttcctctg
     4501 taatatctaa attataattt tttctttccc ataattttag tgtatgtctt ggataatgac
     4561 ctcttacatg tacatcagta aacataagtt gttctctatt agattctaca aatttaatca
     4621 tatcctctgg attgcaagaa taaggataac ttggcatagc tgccaccata catccaattt
     4681 taaactcagg gtttatttca tgacctatct tcacaacctt tgcacttgct acaaattcat
     4741 aatgaactgc ttgatacata acttcttctt tgtcttcctg ctcttcatag attattcccg
     4801 agttagtaaa tgcataaata ggatttgtag ttatactttg attatttatt tcattgaaag
     4861 tcatccagta cttaacttta tccttgtatc tattcattac cacttcacag aagtttacaa
     4921 agaaatctat taattttcta tttctccatc caccatagtt ttttactaaa tgataaggca
     4981 tttcaaaatg acttaaagtt attactggct ctatattgta ttttaataat tcatcaaata
     5041 aatcatcata aaatttcaat ccatcttcat ttggagttga ttcatcgccc tttggaaaaa
     5101 ttctagtcca agcaatactt gtcctaaaac acttatatcc catttcagca aataatctaa
     5161 tatcttcttt ataattttca taaaaattaa tggcttcatg attaggataa tttaaaccct
     5221 caattactcc atctgttatc tttcttgaaa tagtatgagt acctgcagtc atcacatctg
     5281 caatacttat tccttttcca cctttattat atccaccttc aacttgatgg gcagcaactg
     5341 cccctcccca taaaaattct tgattagatt tcatagaaat atctcctcca gtataattaa
     5401 taaacaactt ttattacagg cgttccaatt ttaatatgtt tattttcttt aactattaca
     5461 tctgctaaat catttgtgtt agttagtatt attggtgtaa caatactata cccttcacct
     5521 tctattttat ccttatcgaa agaaacaatc ctatctcctt ttttaacttt atctcctgca
     5581 ttcatatgag tagtataata ctttccatct aacatagtag tgttaagacc tacgtgtatt
     5641 aatatttcta cacctttatc agaggtaatt cctattgcat gctttgtagg aaaaatagtt
     5701 gttattgttc catttacagg agacacaact tctccctttg aaggtattat tgccatgcca
     5761 ttacctatag ttcctgatgc aaacacttca tcttctactt cagataactc tatttgtaag
     5821 ccttctattg gagataatat ctcctctata ccatcatctt catcaaaagc aacttcttca
     5881 ttaccttctt cacattcttc tttactcaat ccaaagaaat atgacaatac tgctcctagc
     5941 actaatgctg ttcctattcc taatatatat cctaggaatg gtgtataaac tggcattgag
     6001 aatatattat gaaatacata tgcattacaa gtaacaccaa atacaccaca tataccacca
     6061 ccaatacctc ctgcaatagc caatactgga atcattcttc tgtatcttaa tataattcca
     6121 taaattatag gttctgttac tcctgctaaa attcctgtaa ctgctgttga acctgctaat
     6181 acctttaatt ctgaattttt tttagatttt ctaaccttca taaataaacc tattgcaata
     6241 cctatttgag caaatactgc tgcaacagct gcaccttcta ttgggtctcc acccattgta
     6301 gccaagtttt gcaaagtaat tggtgtgaag ccccagtgta gtcctaatac tgctaagaat
     6361 ggcatagcta tacctattgc agctcctgca attactctgt taaattcaaa taatcccata
     6421 aaaatattag atattatatc accaacatta gttgctattg gtccaaataa aatcacagtt
     6481 aaaggaacca tgactaataa tgatatcatc ggtactaaga ataattgtaa atcctttaat
     6541 ataatcttct ttaaaagctt atcaacatag taataaataa atatagcaac aaatataggg
     6601 aatacaccaa aagaataatc aattgctgtt actggcatcc caaaaaagtt agcaccacct
     6661 tttgctccaa ttaaagctgt aaaattagga tctaacaaag ctgctcctat cactccacca
     6721 acatatggat ttgcactaag ttttatagct aaggtaatac ctaagaatat tggtaaaaag
     6781 taaaatacag catttccagc tgctgataat atagcataag tgctacttgt atcagacatc
     6841 catttcatag tagttagtat tgtaagcagt gccttaatca ttcctgctcc agctagtgca
     6901 ggtattaatg gagaaaatgc acctgaaatt gattcaaata ttttagaaac tattgaaact
     6961 ttttctccct gttcttcagt attttgtcct tgtaaaactg ctttttttat aatagtttca
     7021 aagtaatcag ttactttatt tccaattacc acttggtatt gtcctccact atttactaca
     7081 gatagtacat ccttcatttt taataactct tctttgttag tttttgaagt atctttcagc
     7141 ttaaaacgta gcctagttgc acaatgaact aaactaataa tattttcttt tcctccgaca
     7201 tattttataa tttcttcaga ctctctttca aatttcatac aatctatcct ccattttcat
     7261 aattttatgt ataaaaatag acctaaatca atacaattag atattaaaaa ctaattatac
     7321 caacttaggt catgcctgcc aaacagtaac aatcctacac tatttacttt atatttgata
     7381 atctatttaa atgcaatgtt aagtacaaca attcattttc gctaattttc catttatacg
     7441 tcttttcaag aaagtttttt actttttttg cacaattgta atcactttta tatttcattt
     7501 ttattatatc taacaatgat gtgtctacat ttgtattctc accacttaat tgccttttta
     7561 taaaacatct taaatgactt ataaaccttg tgtagtcaaa ggaatattca tttatttcga
     7621 ttccatagtg atactttatg atatctaata cattatcaat tatcttagtt aacaataaag
     7681 tttcttgcat atcttttgtt tctaactgag catttgcaaa gtgtatcgta ataaatgcta
     7741 tttcagaatc aggaatattt ttatctaatt tttttcttaa atattctaca gctttcttac
     7801 taaaattata ttcattagga tatatttgtc ttatttccca ttgaagcggg ctttgaaata
     7861 ctaatccttc atccattctt tttaacatat aacttatatg ttctgataat gtgattaata
     7921 tactatcatt aagattatca cccaattctt ctcttcccat ttgaataatt tcacttacaa
     7981 gctcaacatc ttttaaaggt atcctatcta gtagtgaatg taattcactt aatgattcct
     8041 cattattgaa tataaatttc ttttcaattt tatctatttc aactaaatct ttagtaaatt
     8101 tattaaatcc aattcctttt cccattaata ttacttcttg tcctgaatta tctctggcaa
     8161 atacaacatt attattaaat actttcttta taaccataaa ctacctccac tgtaaacaaa
     8221 aagagaccta aaaaagacta ataatacttt aagcatttac tagaattttt aggtcacgcc
     8281 tgattacatc tatctcagta acgatccgtt tatttatctg tatgataact ttattttcat
     8341 tataaaataa taactgtact tagtcaatat ataatttcat gtatatctct actagattat
     8401 tgtcataagt attcactcat tctacttttt ttatatgatt tatataatct aaacatgcta
     8461 aaataagttt tgaagttatt tcataacaaa attaaggtca tatatttttt attatatata
     8521 ctcgattatt tatccatata taaggaaatc ctttattttg taactacaat aactgcctaa
     8581 gttttgattt tcttttatct tttacttctc cttattaaaa aataattctt ttactgttgt
     8641 atctaaggct ctaaacagtt taatcatcaa atcattgttt ggattattaa ccttacctaa
     8701 cttactatat agctttttaa ctgtatatct ttaatagacc ttttggtatg ctaatccatt
     8761 ttataacact aattcttgaa tcaagcatat attaccctta caaagcatat cttataccct
     8821 ctgtagagtt atgatagttg atgaacctga agaaattacc cttacaagac atatctgata
     8881 caacctcact tattagttta tatgtttcag aagtatataa ataacacata cttacttcca
     8941 aattcttaac tgttttctta gaaaattcta atatgatttc cttacatagc gattataaca
     9001 gtgtcttatt agatttcata aaattataca tcctctttac atttaaactt taaattttta
     9061 tctattgttt attaattaac tgtatattca ctatttttta tcatttataa tactcttctt
     9121 attatcttaa aacaattata atctaacctt aatcacatat tatataaata tttttaataa
     9181 acttaataat taaataacta tatttcatta tccatcttat aaatgtttat aaactttcca
     9241 ctattcttct tcaataaatt caaacaaatt ttctactgta gtatcgaata ctttagcgat
     9301 atccatagct aacttcaaag aaggattata tttcccattt tctaagtgta caatagtctc
     9361 acgtcttacc ccaaccaatt tagcaagttc attttgcgtc ataaggagtt tttctctgta
     9421 ttctttaatt tttgttttca tcaatattgc cacttagtta catatccccc ttttatcaaa
     9481 gtatatatac aacactaatt tagataaaga ctgtataaat aaaactgcaa tgaggattat
     9541 acctgtatct aggtttgata tatttatatt acttgtataa ggtgttgcaa agaaaataga
     9601 tattattact atagaatata tcattatttt tattgcaatc ttattaacct ttgatagtat
     9661 aaattcagat gattcatcaa ctaaatcatt atatttttta ttataaattg aataacatcc
     9721 aaaaactatt aaccaactca accctggaag agaagcagtg cttggacctt taaaaatata
     9781 tttagtagct aatacagcta taaaaaaata agttattaca agtaattcca agatgagctt
     9841 tgatcttaca ccagtttctg atttagacat ataaaatcac tccttaaaag ttatatattc
     9901 tctatatttt ttaatttttg ttttcataaa tattattact tagttacata ttcccttttt
     9961 atcaaaatat atatacaaaa ttaattttga caaagattgt atgaataaaa ctgctaagag
    10021 cattatacct atatccaaat ttgatatgtc caaagctctt gtatgattaa ctgaaaagag
    10081 aacagctatt acaaacatag agtataacat tatttttgat gcaatctgat caacctttaa
    10141 caacataagt tttgatgcct catcaactaa atcattatat tttttattat acatgagatt
    10201 gcacacccaa aaagctaata aaattataaa agtaaaccaa ataccttctc ctttaaaaat
    10261 aaacttttgt aataacaagt ctataaaaaa ataggatagc atcattaact ctgaaacaag
    10321 atttgcctta acaccaagct ctgatttaga catataaaat caccccttaa aagttatata
    10381 tttataacac cttatgttat aaatatataa cttttccaaa taaattgcaa tagaatattg
    10441 aaaatattca ataatatctt cataaatata ttttataaaa gattttataa taaacattac
    10501 aatacagtac taaattagtt tatttcatct atcatctcaa tcatatcatt tactaactat
    10561 tattctaatg agcatataac attgaaattt ataattatac aacctaaatg cctcccaatg
    10621 taccctcaaa aacatcattt cctgtaactg aagtataatt ggtatatttt atcactgaaa
    10681 taaaaaaaca ctagtcaata aagactagta ttttcaagta ttttatcatt ctttttttaa
    10741 ttattggaga cgaatcatca gaataatgcc cataatttat ctatatgcaa attctaaatt
    10801 taaaataaag tttcagatga atttactatt ctatgctatg atacaacaga aaaaactgaa
    10861 atctatgagt ggtagcttca tataatccta gttcctacag taatcaaaac tgaggtttat
    10921 tctatatgta actaatgata aattttatta taggagttat tagctctatt tgtgttacta
    10981 taagtagtaa ttatatatat gataattttt taacactttt ctgttggcta tatagaaagt
    11041 ggtttagaat ttaaatttaa ataaaattta aattcaccaa aaagaaatct atattagaaa
    11101 ccactactct attccaccag attgtaattt tttgttcttt atatcacaat gctataccat
    11161 attaaattga aaaataaatt ttaaatttat ttgttacaat tcattaattc aaaaaaatat
    11221 tttgagtcat gagttttgta aagatttagc catgaattta atgcttctga ttcaaaaaca
    11281 tctaaaagtt tcaatacttg aaacgtaaat tccaacggag cgattcctga agccgtaact
    11341 aaattttcat cactaactac agattccgtt ttataatatt tttctccaga gtaatttggt
    11401 attaccattt taagaaactc taagtcatta cttgtatgat ttctaacatc taatagccct
    11461 tttttagcta ggcctaatgt agctccacat atggctgcaa ctatagaatc ttgtattaaa
    11521 gcttctttaa ctttttctaa tattatctca tgattcatgg ataaccaagt atttccccct
    11581 ggcaaaatta ataaatcctt actttccata ttaaactcat caactgtaat ttcaggtaaa
    11641 acttttaacc cacccattgt tataatagga tttttatcaa ttcctacagt aattactttt
    11701 agtggcttta atcctttttt aaaatatctt cctgtattta attcagcagt taaatatgcc
    11761 acttcccaat ctgacatagt atcaaataca tacatatata cttttctatt tttcatcttt
    11821 tttactcctt atgtaattaa tttatatatc taattataaa ataacttaac tgacatattc
    11881 agtcagttaa gctattaaat ttgataataa tcaattaatt ctagtaaatc ttcaattatt
    11941 ttcttcctaa ggcttagtgg ttcaattact tgtattgtct ttctataagg taaaagtata
    12001 tttggtatgt acttgcatat tacttcctct tcgagcataa atattatttt attaggtgtt
    12061 ctttctttca agtagtgccc taaaaaccaa tgttggctta aactatctaa agaatctata
    12121 ctcccttcta caactaattg aattatattt tgttcatctt ttatttcagg tataattcct
    12181 tttataaaaa attcacttgc tgaaaattct gtcggtctgt taaatgtatt tgatgtttct
    12241 atgatttcac taatcctatc cactctgaaa ctacgtacct catttctcat attacaaaat
    12301 gcaactacgt accaattatt attccagtga acaattccat atggatttat aattctatat
    12361 tttgattttg ctttacgacc tgcatgatag ttgatttcta cagatagctc ctttaatata
    12421 ctattctcca attttttaag tactgattct atagacactt tcccaattgg ttttattact
    12481 tcaagggctc ttaagtggtt tgttaaaata tcttcttggt tttgatttga atacattttt
    12541 aactttaata tcgatttatc taatgcttcg tctaagaagt atccttcttt tttagcaaat
    12601 agagctgcat gaagtagaga tttttgctct tctatatcaa aaaaaagagg tgctttagta
    12661 aaattattta aaaggctata cccaccatta tgacctgtat ctgaaattat aggtactcca
    12721 ctcgcagaaa gtacatcaat atatcggtac actgttctta tatttatttc taatttttca
    12781 gctatctgtt ttgctgtaat ttttttgtca gagtttaaca tccacagtat agacatcata
    12841 ttgtcaattt tagccatgta gtttcacctc tttctatttg ttatataaag ttattatata
    12901 actttttgat tgatttttta caaaaaaaca ttagtaaact gctaataatt tatactaaga
    12961 tttatttcaa atatttatag atagtctttt tctatagtta agttatccat taaattacat
    13021 agtagtatat ccattttagt tgatatattt tcagctacct cccttgaata ttattacaat
    13081 aagtctaaat cttctttctt ccataaatat attatgagga aagactatca tctttaatgg
    13141 tttaatagta taaaagatat taatttaaaa aaatctattt ttatatcaga tgggtattgt
    13201 tttttataaa actgaaaaaa tcacctaata gaatctatga ttttttaaca taacctccat
    13261 aaattagtac caattatcac ttcaaattca aatttttatc tgaagaatat acaatcatat
    13321 ttccatactc aacataatat tgagtgtttg gcttgtattt acaaattatt attctaacaa
    13381 aaacaactat acatagatta aaatcacctt ctaaacataa ttattaaaat ttaattaatg
    13441 atatcaattc cattaatctc ttttaataaa aaattctctt ttctatatga aaattatttc
    13501 aaaactttta agttaaatac aaaaatgact ataaatagat ttttatttac acaaatctat
    13561 ttatagtcac tatagtttat ccaataaaat aataaaataa atatgtacac aataaatgtt
    13621 atttacgtct taagtattta tttgttagat ttttaattaa aaaaaacaag ctaatgaaca
    13681 ttaatgctaa atctagcatt atattacttt gatctcctgt ttgtacattt tgacctacat
    13741 tactatctaa actttcatct gtatctatat ttatatctgg ttttccattc ccatctgtat
    13801 ctatatttat atccggcttt ccatccccat ctgtatctat atttatatcc ggttttccat
    13861 ctccatctat atctatattt atatctggtt ttccatctcc atctatatct atatttatat
    13921 ctggttttcc atctccatct atatctatat ttatatctgg ttttccatct ccatctgtat
    13981 ctatatttat atctggtttt ccatctccat ctatatctat atttatattc ggttttccat
    14041 ccccatctgt atctatatct ggtttgctat ttcctccatt atctgggtct ggtttactat
    14101 ttccaccgtt atctggatcc ggtttgctat ttcctccatt atctggatct ggtttaccat
    14161 tgccaccgtt atctggatct ggtttgctat ttcctccgtt atctggatcc ggtttgctat
    14221 ttcctccatt acctgaatcc ggtttgctat ttcctccatt acctgaatct ggtttgctat
    14281 ttcctccatt acctgaatcc ggtttgctat ttcctccatt acctgaatct ggtttgctat
    14341 ttcctccatt atctggatcc ggcttgctat ttcctccatt atctggatct ggcttattta
    14401 gatttggatt acctgtatca taacctcctg agccagtatc tacattttta tcaggcttcc
    14461 aataatcatc tactccatca ttatctgtat ctattgctgg acgattataa tctgtatcag
    14521 ggtttccatc tccattggta tctatatttt tatcaggttt ccaaatacca tctccatttc
    14581 caccttctgt actaggcttc cattcacctg tattatcagt atctatgtta atatctggct
    14641 tccaatcacc actcgtatct atattgatat ctggccttcc atcaccatca gtgtctatat
    14701 ttatatccgg tattccatcc ccatctacat ctacatttgt catatctaac tttaacttct
    14761 tttcatcctc actaccttca atgtaattta tatcatctcc aatatatttt acagtaatag
    14821 tttgttcagt agtaaaaata tgatgattgc tatctaaagg tctccattca atagatgcag
    14881 tactataatt atgactctca ctacccttat tatgagtttt taattctact ggatctttat
    14941 attcctttcc attaatatag aattgtacgt aacctcttgg agaggcacaa tctggtcctt
    15001 ctttcttatc tggtgaaata gtaacattta atgtaaacgt atctgttggt ttaaaaacat
    15061 cagttttagc tttagactct tctacaacta atgaagtttc tgtatcagca ggaataattt
    15121 ctgccttata atatgctcta tgcccccaat aagcttcatt aaaaggatat acatttgaaa
    15181 actgtgttga tgtaatgctt aattgatatt ttccagcatc aacttgcttt gtaaggtcat
    15241 cacttcctaa atttgtatct ggttccattg tttcctcatt taaacgttga gaactaatag
    15301 tcataaattt tttatctgtg agtttttcac ctttaggagt aattattgga atacccccat
    15361 cttttaatat ccaatctaaa aataattttt ctacatcttc ttcaaatggt aatccatcat
    15421 atcttttaat aatcaaatat ggtggagtat catcaatagt aaattcttca tattgcttta
    15481 aaaattgaga acctgaacct atatctttaa cataaaaatc atctggtttt atatttcctc
    15541 cagtaatttt atctataaca actaaattaa cagatacttc agatccctta aattgatcag
    15601 aaaaccataa gtaaagaacc ccttgatcat ctgtataact aggtgctcca taaggataaa
    15661 tttttccatc aattagcatt ttaaaatcat caactaatgt agtactaact ttatcttgtc
    15721 ctaaatactt taaagatatt ttattcataa aaacttttgt tttcttttct aaatcaccgt
    15781 atgcaactgc tccacctaaa cttttgaatg ttgttgcttt taatgaacca cctgttacta
    15841 taacatctcc tgttgtagca cttaggtcaa aaatccaatc tcccactcct gtcatataag
    15901 gtaaaatcgt tcctccagtt acaagtatac gatatcctct atttgttcct ggattaacac
    15961 atccatcccc aaatgcacta ccatgtgcac ctccatatga ataggaaagt ccaccattaa
    16021 ttgtaatatt tccactataa ccagaacctg tgctaggatc tactactgag ggacttaatg
    16081 cattctttgt tgttgtatag tatcctccac ctattccagc tccatggtat gcacctttag
    16141 cagttatagt accaccattg attgtgatat ctccaccact accattaccg cctccaccta
    16201 ttccagctcc atatcctgta ttacgactcg attcttgacc tatagcagtt atagcaccac
    16261 cattaataat aatatttcct ccactttcag tatttccacc gccaatacct gcagaataat
    16321 aatttccaac aacttttaaa cttcctctat tattactatc tagtagtgta aggcgatctc
    16381 ctttttttaa cttgtctcca ttttctagag ttacatctct aggaattttt ccaccctcag
    16441 gaactataaa ctctccttca atatctctat ttacaacaga atcatcaata gttagagtag
    16501 taccttttcc tgctctcata cctggataat atacatcacc tgactctagc ttgttttcac
    16561 taccatcttg taatataaca tgtgcatatg caccaccatc tttatctggt gttaaagtaa
    16621 atgggctatt agatgcagtt ttaatattta ctccatcaaa tattaattca gcttttacat
    16681 ctttaggtat aacaatccta tgtgttgttg gagtatttgg attattattt tgaattgtca
    16741 tttgttttcc actaagaata gttaaaacgc cacttgcaaa tttaaaatcg acatttattt
    16801 ctccaccact aattttaaag tctcctacag atgtaggatc ttctgcagtg gcttcttcca
    16861 caactaatgc attcatatct tcttctgcat ttcctgcatt agaatcttct tctgcatttc
    16921 ctgcattagc atcttcttct gcatttcctg cattagcatc tccttctgca tttcctgcat
    16981 tagaatcttc ttctgcattt cctgcattag catcccctcc tgcatttcct gcattagcat
    17041 cccctcctgc atttcctgca ttagcatccc ctcctgcatt tcctgcatta gcatcccctc
    17101 ctgcatttcc tgcattagca tcccctcctg catttcctgc attagtatct ccttctgcat
    17161 ttcctgcatt agtatctcct tctgcatttc ctgcattagt actttcttct atagtatctg
    17221 cgaaagtatt tgacgcatta aatgatgcta ctgatccaag tcccattgag atacttaaaa
    17281 tcattgatag tgctttcttt ttacattttt gttgcataaa ttccccctcc ccttaataat
    17341 ttttaatata cactgtatac atccttatat acactctaat aattcttaat atacactgta
    17401 tacatcctta tatgcactct aataattttt aatatatact taaaacttat atttgtcaat
    17461 gaatatactg tttttttcaa ccttatata
//
LOCUS       DACULQ010000074        17407 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.074, whole genome shotgun sequence.
ACCESSION   DACULQ010000074 DACULQ010000000
VERSION     DACULQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.074"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..707
                     /locus_tag="JA154_09890"
     CDS             <1..707
                     /locus_tag="JA154_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7996174.1"
                     /translation="YIFNDDLNCYSCIKCTKTYEVGEIYKIGNRFFEASKAIELHMEL
                     EHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVGVSTSTIRHQKFIFR
                     EKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVTEEERKHILDTSFES
                     MNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILESIYDDFATIRRYLIE
                     YGFMSRNKECTKYWLT"
     gene            768..1442
                     /locus_tag="JA154_09895"
     CDS             768..1442
                     /locus_tag="JA154_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HAT7996175.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            1811..3157
                     /locus_tag="JA154_09900"
     CDS             1811..3157
                     /locus_tag="JA154_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996176.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3184..4584
                     /locus_tag="JA154_09905"
     CDS             3184..4584
                     /locus_tag="JA154_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996177.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GESGHYKNLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            4794..6230
                     /locus_tag="JA154_09910"
     CDS             4794..6230
                     /locus_tag="JA154_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996178.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            6574..7518
                     /locus_tag="JA154_09915"
     CDS             6574..7518
                     /locus_tag="JA154_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996179.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7687..8619
                     /locus_tag="JA154_09920"
     CDS             7687..8619
                     /locus_tag="JA154_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HAT7996180.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENTLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8724..9191
                     /locus_tag="JA154_09925"
     CDS             8724..9191
                     /locus_tag="JA154_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996181.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9435..10289)
                     /locus_tag="JA154_09930"
     CDS             complement(9435..10289)
                     /locus_tag="JA154_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAT7996182.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10449..12419
                     /locus_tag="JA154_09935"
     CDS             10449..12419
                     /locus_tag="JA154_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter"
                     /protein_id="HAT7996183.1"
                     /translation="MKMLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKS
                     LKEVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVL
                     LKSNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDK
                     RQLTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRE
                     RKFEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKF
                     SILLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETA
                     YDKDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDI
                     RINDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIE
                     KVLSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTS
                     KKYKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYT
                     SFQSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEV
                     FYDFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12419..12880
                     /locus_tag="JA154_09940"
     CDS             12419..12880
                     /locus_tag="JA154_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAT7996184.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12883..13203
                     /locus_tag="JA154_09945"
     CDS             12883..13203
                     /locus_tag="JA154_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAT7996185.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13204..14319
                     /locus_tag="JA154_09950"
     CDS             13204..14319
                     /locus_tag="JA154_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAT7996186.1"
                     /translation="MEGNVMLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGS
                     FYNIPDIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPG
                     LVGGMIAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLIT
                     VVLINYVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTS
                     LALLTSGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSE
                     GAIPFAVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSI
                     AVGTVIVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14439..17039
                     /locus_tag="JA154_09955"
     CDS             14439..17039
                     /locus_tag="JA154_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAT7996187.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDENIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYHIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6926 a         1781 c         3003 g         5697 t
ORIGIN      
        1 gctatatatt taatgatgat ttaaattgtt atagttgcat taaatgtaca aaaacatatg
       61 aggtaggaga aatatataaa ataggtaata gattctttga agcttcaaaa gctattgaac
      121 ttcatatgga attagaacat agtgattatt tagagcaatt gcttaatagt gattcaaaat
      181 acaacacatt gactgaaaat caaaaacaat tatttcaatt atttattagt gatttaccag
      241 ataaggagat agcaaaacag gttggagtat ccacatctac tatacgccat caaaaattta
      301 ttttcagaga aaaagcaaaa caggctaagc tctatttagc actatatgaa tctgtatttg
      361 aagataaatc taataaaagt agtactataa taccaatcca tgaacatgct cgaatggtag
      421 atgaaagata tgtagtaact gaagaagaga gaaaacacat tttggataca tcatttgaaa
      481 gtatgaatcc tttaaaatta aaagtatttt cttcaaaaga aaagaaaaag gtcgtaattt
      541 tatctaagat tttggaacaa tttgaacgtg ggaagaaata ttcagaaaag gaagttaatc
      601 agattttaga atctatatat gatgattttg caacaattag aagatatcta attgagtatg
      661 gttttatgag tagaaataaa gaatgtacaa aatattggtt aacataatta aaataataaa
      721 ttttactaat gtgtaatgtt atttttatct agggggagga gtcaaatatg aatgatgaat
      781 taaaatctat tttatacaat aaaggtgtag acattgttcg ctttgttgat atatctgaat
      841 ttccaataaa tcaaacacaa ggtttttcta aagcaatttt attttgtata gggttatcaa
      901 aaaaatttat tacagatata tacaataact tacctacaga tagtgatgag tttttggaaa
      961 aagaggagaa agtagaagaa ttagctgact ggatttcaaa atatattcaa aataaaggat
     1021 atagagcata ttctcaatcg gagaaaaaca atctagaaca tggatatttt gaaaaagcct
     1081 atattaatcc agaaatgcaa tcagggatta gtcctttacc acataagaca atagcaaata
     1141 ttagtggcat tggatttatg ggaaaaaata atttgtttgt aacagaggaa tatggatgtg
     1201 cttttagcat gtgtacagta cttacagatg ctccaatttc tgttgaaaga tatcctttga
     1261 ttgattcaaa atgtatggat tgcaatgttt gtgtagagaa ctgtcctgca aaagctatac
     1321 atggaaatga atggacttta cctggaaaaa gagaatctat tattgatgtt tcaaagtgtt
     1381 tttgtgtatt gaggtgtatg atgagttgcc catggtcatt aagatatgca aatcaaaaat
     1441 aactttacta gagaaggtat taaattgaat attataaaat ttttaaaatg taagattata
     1501 taagatttct caaaataaaa atttgtatag aacactcaga aatgagtgtt tttttattgg
     1561 gattaaatta tattgataca aagcttaact attttaatca ttatataaaa tttgtattaa
     1621 aaaattaaga ataacttggt aatagtgaaa atataatgag attataattg atttataata
     1681 taactataat gatattgtaa aaatgaaaat tgtcctctca aaattcccaa agagagtaaa
     1741 agtcacttca gttatagtca aaaaaatttt tctgtgaaaa gaatttagat ttgaaaagga
     1801 ggaattaatc atggaaattg aaactaaaac tccagattgg attaatgtac tagaattaaa
     1861 cagtaaaata gatattactg ggaaatatga tgtaagtaat atgatacaaa atattgtctc
     1921 tgataagtca ctagaaggtt ccattatata ttttccaaaa ggtaattact tatttgagaa
     1981 aggtattaaa attagtcaac aaataacact tcaaggagat tcttattatg gaggaggtaa
     2041 tcaagtatca aatttagata aaaaacaatc aatcatagga acaaccaatt ttataacccg
     2101 aggagtttct aatatgagta ttattacatt gtcaggaaca agtcaatgta tcaaaaatat
     2161 taatttttat tatgatagtc atgatataga gaaaataccg cctaaaaatg tatctgctat
     2221 tacagaatat ggagaaacac aaggactatc tcattttgaa catctgttta tttctggatt
     2281 ttcagggata ggaattgaaa ttccatacta ttcaactggt aatgatatta ctgttagttc
     2341 atgtggtctt ggtatgaggc ttggagagaa gtcaatgctt tctagtagca agatttacga
     2401 atgtaaaaat ggaatggaga ttactactgg tgttagctta aataatgtga gaatagaaga
     2461 aataaaagaa ataggtatta ataataaagg atttggtttt aacctgatta tgaatcttat
     2521 ggtcgaccaa tgtggatatt gtggatttat gtttgaaaaa atgtgctata gccagataac
     2581 tgccagaatt actagatgcg gtcagtatta taaaagtgtt gattataatg cttatgagaa
     2641 tatggaagat aggaaagaag aagcttattc cattttttat ggagatattt tagaaaattg
     2701 caatattgta ttgatgaata gtaatattga taccttggaa aatgattcac ttgatgaaca
     2761 taagatatat gtaatcaagg caaatgaaac aaacaatgta atgctaacat gttatgctga
     2821 agcagataac tttatacaat gtgcaaaggg caacttgctg ttagaaaatg gtgaaaatac
     2881 ttataaattt tataatggtg agatttgttc tgtaggaggt gttcaaattt ctgatataga
     2941 tgaaaaagat ttaatcaaaa taatagagaa cactttgtat atagatgata aaggtaagta
     3001 tttaatggtt cctagattaa aaaagaatac tgtaatatat tcagtactag attctacaga
     3061 agaaataaat aaattacatg gtggaatatg ggaacagatt gatgtaaaag tattagctgg
     3121 ggaatccatg tactattata aaaagataaa agattaaatt atcaaactag gaggtagtga
     3181 ttaatggaag agtctataat aattaaagat ataagtggct ggattaatgt ccttgattat
     3241 ggaatttgta atgatgaaac aaaagatgta agttttaagt tgcaagagat tatggatgct
     3301 gtaccagaag attctattat atattttcca aaaggaaaat acttatttga aagtggtgtt
     3361 aagattaaga aacgattaac tttatgtgga gattcttata ttttggcgaa tgttcctaaa
     3421 aatactgctg gagtaacaca atttactttt aaaggaaaga aaaatcctaa aagtgaaaat
     3481 atagcaatta taacagcaaa aagtgtaaga cactgtatta agagcatttg cttttattca
     3541 gatagttgtg atagagatat gacatctaat catgaacctc caacagatgg aaaacctaga
     3601 tttcatcata atgtaattat aaattataga aatatatcgg ctattgtatg tgaagataaa
     3661 cagggagaat caggtcatta taaaaatcta tcttttagtg gtttttctgg aattgcactt
     3721 gatatgccta ataattctac agcaaatgat attacaattt ttacatccgg acttggaatt
     3781 agtacaggag aaaattcaac tattgctaac agtaaggttt ggggctgtgt tgatggaatg
     3841 ataatttcta ctggaacttt tataaatggt atgagggtag aagaaataaa aaaagttgga
     3901 attaaaaata ttggtagagg tttaaatttt attacaaata tcacaattga ccaatgtgga
     3961 tattgtggag tttggtttga ttctatatcc aatgttcaga tatcaggaaa tatcacaagg
     4021 tgtgggcagt actattatga tgtaagctat gataattatt taatgttgga taatagagtt
     4081 aaagaagcat attctttatt ctatggtaat ttattggaat caagtaatat agttttatca
     4141 aataccaata aagataattg ggaagatttc aaagaagata agcataaggt gtatcttgta
     4201 gaagcacttg aaaccaagaa tgtactatta acatgcaata tagatgctac tgactggatt
     4261 gttaagtcag aaaaaggaaa tttaacgtac aataatatgc gacaaactgt tatattttac
     4321 gatggaaaac taagtagtat caatggaatt ggtatatctg atgaagatta tggggataaa
     4381 gtcaagataa aaaaaggaaa tatatatata aatagtaagg ataaggatat taagatgcat
     4441 aaaccagagg taggtgacat tatgtcaaca attattaaag ataaaagtat tctttctgct
     4501 cattatgggg gaacatggga gaaaattggt gaaaaaatag agtttggaat aactgtacac
     4561 tattataaat tgatagagaa ataagtaaat acgtagtatt tgaatagatg tattttattg
     4621 gagagctagg actatagttt taatgtaaat ggtttggtaa tagcatggat tgaataatat
     4681 aaaaataata taaaaaatta atttgattat gtaaaattct atgaagtagt aaaactgtga
     4741 ctttagtcat aggaggttca aactaagtaa aagggaaagg aatatataat attatggaag
     4801 gaagaactgg gattaaagat ggaagatgga ttaatgtagt agaaagaggt attaaaaatg
     4861 atggttcgta caacgtaggt cctgctttgc aggatattat tgatgaaata gaaacagatt
     4921 tttgtgtctt atattttcca aaaggtaaat acctatttac aagtggtgtt aaaattaata
     4981 aagaaattgt attgcaaggt gattctaatt catctacagg cactacacaa tttataactc
     5041 gtggagtaga aaatatgagt attattacat tgacagggaa aaaacaatgt attaaaaata
     5101 taaattttta ttctgatagt tgtgagatac aggtaaataa agaaccaccg acaaaaggta
     5161 atcctaaata tcattatgag atgattatta ataagaatga aaagaatgtt gagatgaata
     5221 atgtatcagc aatcctgtat gataacagta agaatatgat taaaggtcta ggtcattatg
     5281 aaaatattta tataagtggt ttttcgggta caggaattag gataccatat tactcaattg
     5341 taaatgatat tacagtttca tcttgtgggc tgggtattga tacaggaata gacacaatta
     5401 tttctaatag tagaatatct aagtgtcaaa atggaatgag gattagaact ggaactagta
     5461 taaataatgt aaggattgaa aaaatacaga aagttggtct tataagtagt caagttaagg
     5521 ggtatgaggg gtatggaagt tataaaatta ataatattac gattgaccaa tgtgggtatt
     5581 gtggcttttc atttgattca atgaaagata gttatttttc tggtattata agaaggtgtg
     5641 gtcagtatta ttataataca gattatgaca catatttaag tattaaagat agagtagaag
     5701 aagcatattc tttattttat ggaaattatt ttacagaatg taatattgat ttagttagtg
     5761 ataataagga taactgggat gatggcttag aatataaaca taaagtttat gtatttaaaa
     5821 cccttcaaat accaaaatta acattgagat gcaatataga tgcctatgat tatatcgtta
     5881 aatcggtaca tggaggaggt aatttaatgt taaaaaataa ttttaacaat tatatattta
     5941 ttgattcatc taatccagaa gttattaatg gaattaattt taattatcaa aagaatggaa
     6001 acttgttaag gttaaataat ggtaaattaa gttataaaaa tggaaaggaa gaaacagcta
     6061 tattcagacc taattatggt cttattgtag catcaacaga ttcagaatgt gaacaaatag
     6121 agactagcta tggaggaaaa tgggagaagc ttggttctga agtaattgga gaagaaacgg
     6181 tatattatta taaacatcat gaaccagata aaacaaacca aatacaataa aatttgtatt
     6241 atataatgat ataaagtttt aatatatagt gtttaccttt aatgtaatat tggtatattt
     6301 gtatagtatt aaatttgtta aaaagtaaaa ttgtagggtg cttgaaaata gcaccttttt
     6361 tagtttcatt ataaaattag ttttgaaagt ttaggaataa tgtatattta tttttaaaga
     6421 attataacct tattaaggat attttaaatg ctagaaatga atcagtaaat catactggaa
     6481 ttatcttaaa tgttttgatt aaattaagta tttagagcta taattatatt aaaaatatat
     6541 tagaacataa ttatattact atggaggtta aatatgatag taagtgtgag tagaagaact
     6601 gatatacctg ctttctatag taagtggttt tttaacagac ttaaagaagg ttttgtttat
     6661 gtaattaatc caatgaatcc aaaacaggtt agtaagatag agttaaatcc acatactgtt
     6721 gattgttttg tattttggac aaaggatgta actccaatgt tatatgattt aaataaatta
     6781 aaagactata aatattattt tcactatact ataacatcat atggaaaaga agtagagaca
     6841 tgtattttag ataaaagaaa ggtaatagat tcatttaagg aattgtctag aaaaataggc
     6901 aaagaaagag taattttaag atacgacccg attttcttaa gtgaaaagta cacagtagat
     6961 tatcatatca aagcatttgc gagtttatgt aatcagcttg atggatttac agaaaaatgt
     7021 ataataagtt ttatcgattt atataagaaa acaaaattta atactaaagc attacatata
     7081 aagccaatag aaattacaga aatagaaaca ttatcaaaag agatttctaa gattgctcat
     7141 aagcatggga taacacttga aatgtgttct gaagaatata atttaagtaa atttgggatt
     7201 ggaaaaagta agtgtattga tgatagatta atatcaaaaa ttataggatg tgaagtagag
     7261 gtaaaaaaag atagtacaca aagagatatt tgtggatgtg ttaaaagtgt cgatatagga
     7321 caatacaata cttgcagaca ttattgtctg tattgttatg ctaactttaa ttatgcaaaa
     7381 gtagaagaaa actgtaggct ttataaagaa gataataaat tactcataga tactttaaga
     7441 gatgatgcaa aaataagcat tagagatatg aagactatta aagtagataa gaataaacaa
     7501 attagtatat ttgattaaaa taaatatact aattacctta atatattaag aatatagata
     7561 cttttgataa caaaattaaa cttaatagat attttctata taagaaagat ggtcataata
     7621 tgtcaatttg accatctttt tgatataatt aaaacacaaa taatttagta acaaaggagg
     7681 cagaacttga atttattaaa aaaatatcct atacctattg caagtttgat tttgtcaatc
     7741 tttactttag gtaacttatt acaatcttat agtgaatcat tacgtaatgt aattggattt
     7801 ataggattta ttttgtatgc aatttatata gtcaaaataa tattacttag aaagggtgtt
     7861 aaagagcaat tagagaacac tttaatatca agtacatttc caactataac aatggcaaca
     7921 atgatatttg ctacatatat aaaaccatta tctttagaat taagtattat gatttggagc
     7981 ataggaattg tgggtcatct tgtgttaata gtcttattta gtaagaagtt cttagttaaa
     8041 ttctcaataa aaacggtatt tccatcttgg tatatagtat atgttgggat agttgcagct
     8101 agtgttacgg caccagcatt taatcaactt ttgattggca aaattgcttt ttggatagga
     8161 tttgtatctt atatagttct tataccagta attttaaaga gagtatggat aatcaaggaa
     8221 atgcctgaac catctttacc aagtatagct atattagctg caccagggtc actattattg
     8281 tctggatata ttaattcttt ttcagataaa aataatgtag tattatactt gttattcata
     8341 ttatcaattg tattttatat tatagttttg ggatatttac cgaaattatt aagattgcct
     8401 ttttatccga gttttgctgc atttacattt cctatggcaa taagtgcaat gggtatgaaa
     8461 ctaatgaatg gttatttaac taaatcaggt cattttataa gttggatttc atatgcttct
     8521 aagttagaag agataattgc tacagtgatt attttatatg ttttagtttt gtttattata
     8581 tatcttagta aatctgaaac tacatctaaa gctaaataag aattattaaa tatgtgtatt
     8641 taataggaat attgatataa atatatgaag ataagataaa ccatagaaac ttaatttagc
     8701 ttaattttat tggaggaaaa caaatgaaaa caaaagaaga atatttaaaa gaagttagaa
     8761 acatatcaga agaatacttt agaaaaggag agtttttttg ctctgaagca gttctccaga
     8821 caataaatga tgctttaggc caacctttaa gtccagagat aactaagtta gcatcaggat
     8881 ttccgatagg acttggaaag gcacaatgtc tttgtggagc aatatctggg ggagagatgg
     8941 ctttaggtat agtatatgga agagttcatg gtgaagctat gaatccaaag atgtttgagc
     9001 atgctaaagg tttgcatgat tatattaaaa aagagtatgg tgcaacatgt tgtagagcta
     9061 ttacaaagaa atgggatggt gataacttta tgtctcccga aagaaaagag cactgtatta
     9121 aaataacagg tcaagttaca gagtgggttg cagataagct tatagaagat gaaaaactta
     9181 atatcaaata gtgtaatatt agttggtgaa tttataaagt taaatttact tatagtataa
     9241 gatacttgat attctcgtat tgatagaaaa atttatagca tatgtattta atgaaatata
     9301 cttatattta gagtgtggtt taatttcata aatacatatg ctattatttt aagtatataa
     9361 gcaaatttat ttaaataaat agaaacagtt aatttatatg tacaaaacta tatatgatta
     9421 gtgtaaataa atacctattt tataatactc aacataaaga caccagacat gattagtatt
     9481 ataccaataa aaatagcaag ccattctttt ttgttcttct tttctttaaa aaatataatc
     9541 cctcctaaag tggctactat cacacaactc tgagagatag aaaaacttat tgtaactccc
     9601 aatgtattag tcgttatgaa catacctaaa tttgctattg accacaatat accagtaatt
     9661 aaattgaata aaacattttc cctatataca atctctttag aaaaatactt gacaataata
     9721 gatgaagtta tcaacatacc aacagattga ggtaatataa ttctatatcc ttcaattaaa
     9781 aatatttgat tagttacaac atataatgat aaaaatagag atgataataa tacaactcca
     9841 acacatttta acatattttt atctgtagat tttttagttt gataatttgt gagaagtata
     9901 ccaagtatta tacataatat tgataaagaa ccaatcagta acattttaga actggtccat
     9961 tcattaaaca agattacagc taataatgtc gtaaaagtta attgtgaacc atttgaaatt
    10021 ggcattactt tagatacact agcaatttga attgctttaa actgcattaa ttgtcccaaa
    10081 ctccagaata tgccagaaat catacttata caaaatgaaa aaaatgtaat ttcaggatgt
    10141 ataaccagag ttattatagt ggaaaatata aacgcactta tggatgtacc taaaagctgt
    10201 tcatgaggag aacttttctt tagatttgca actatgggca tcaatcccca tcccattaca
    10261 ggaagtaaag caataattat tgtcagcatt aaactatttc ctttcatatt aaatttagac
    10321 agcttttaat ttatcatcta aaattaaagt gttcaataca taaaaattac cgtatttaat
    10381 caggaaatat tagtagtttt ttagaaaaac attttccatt aaaataaaga caaagggaag
    10441 gagtaaaatt gaaaatgtta actgatatga ttcaaaatcg tgaaattaaa ataataaagt
    10501 ttttattaaa agaaggtcat acaactgtaa aagcaatagc agatgattta gatgtttcag
    10561 aaaaaacagt ttcaaaatct cttaaagaag tagaagcagt gcttaaaaag ataggtcttt
    10621 ctttaataag aaaacctaag ataggtgttt atattgatgg agatttaaaa aaaatacttc
    10681 cttacctaga taataaagga agtcaaattc ctacaaccag agaggagaga gttatctata
    10741 tatattcagt acttttaaag agtaatgatt atattactat acaacaactt tctgatgaac
    10801 tatatataag tcgtggaact attgaaaggg atttagcaga agttgaaaaa ctgctaaaaa
    10861 acgaagggat ttccatatat aagaaaccta gtaagggaat aaagttaaat ataagtgaaa
    10921 gagataaaag acaacttact gctaagttta tacataaatt ttggagtaga aactggtata
    10981 ttaagcaaga gcaagaaagc ttttatcaag catttgaaaa gatacaagca gatgtaaatg
    11041 gtattttttc ggaagatgat ttatcaataa ttattgatat tcttaggaat ttcagcagag
    11101 aaagaaaatt tgagttctct gattatgaat ttcagtcatt agttattcat ttggcaatag
    11161 ctgtagagag aattagaaaa ggcgaatata tagaagaaag tttgtatact ggtaatatag
    11221 aaaataatca aatgtcaaat accaattatt tagcaaaaca attagaagaa aaattttcaa
    11281 tattattacc aaaatctgaa attggatata taaatttaca tttagtagcg tctgaccaac
    11341 atagagatac taatgaacta aaaaatactt caactaatcc ttgtgagaca agtacaaata
    11401 atatatcaaa tcttcgggaa attataaaaa atacattgta tgagacagct tatgacaagg
    11461 atttaattga tggattgact tcacatgtac attcatcaat aaatagactg aaatatggac
    11521 ttagtattaa aaatccatat gtaaatacaa tcaaacaaaa cttttcttta tcatttgagc
    11581 aagctttaga tttaaagaaa gtaattgaat ttatatatga tattaggata aatgatgatg
    11641 aatgtgctta tatagcatta cattttgaag cttttagaga aagaatgaaa gaacttccac
    11701 aaaatattag agtaattcta gtatgcagta caggattagg ttcatctagg ttattgtctg
    11761 caagaataaa aaaatacttt ccaatgataa agattgaaaa agtactttcc gtacaagaat
    11821 tagtatccag taaaattgat gcagatttag taataagtac tatttattta gagttagaaa
    11881 caattccaac catagtagtt agtcctattt taaatgaaag tgatataaga accttggaaa
    11941 gaaatataca aaatgtttca agttgtactt ctaagaagta taaatctttc agaaacttaa
    12001 tctttgaaga aaatatattg ttgaatattg aagcttcaaa ctatgaagat gcaattaagt
    12061 atataacaga taacctaata aaaaaaggtt ttgcaaaaga aggtatagct aaaagtgcaa
    12121 ttgagagaga aaatttatct tacacttctt tccaatcaat agctacacct catgctaatc
    12181 ctagttatat taaaaaatct aatatatctg ttgcaacgtt aaaaaatcct attacatggg
    12241 gaaatgaaaa ggtggaagtt attttcttta tttcattaga aaatgacaaa aacctagagc
    12301 ttgatgaaat atatgaagtt ttttatgact ttattgacaa taaaaaaaat ataaatgaca
    12361 tattaagtgc aacttccaca aaggaagtgt acgatttgct agtggaggag agtatatagt
    12421 gaaaatatca gatgtattga aaaaagagca aattgttttt aatcttgata caaatacaaa
    12481 ggaagaaaca ataaaaaaat taagtagtat atttgttgaa aatgggattg tggacaatga
    12541 agaagaatat gttaagtctg ttttggaaag agaagaacat tcaactacag gtattggtaa
    12601 tggtattgct attcctcatg ggaaaagtaa tgctgtcaaa aaatcttcta taatatttgc
    12661 aaagactaag cataagattg aatgggatgc tttagatgaa aaaccagtag atagtatttt
    12721 cttattggct ataagcaata ttgataagga tagcaatcat cttgtactat tatctaaatt
    12781 agcaactaaa cttatggatg atgacaatgt agatgcacta aaattagcca attcagaaca
    12841 agaaataatc aatatattta gtgaagaagg agagatgtag aaatgaaaag aaaaattata
    12901 gcagttacag catgtgcaac aggagttgca catacatata tggcagctca agcattaaag
    12961 aaagcttcaa aaaagatggg tcaccttata aaagttgaaa cacaaggagc tacaggaatc
    13021 gataatgagt taactacaaa ggatgcacaa attgcagaag tagtaatatt tgcagtagat
    13081 actaaagtca gaaatgctga acgttttgag ggaaaagaaa tactagaagt accagttaat
    13141 gcacctataa aggatgcaga gaaagtaata aatgatgcat taaagttaat tgacactaaa
    13201 taaatggagg gaaatgtaat gttgaaaaag atagtctctg agttaaaaaa gcatgtctta
    13261 actggaatct catatatgat tccattagta attgcaggtg ctatgataat ggcgatatct
    13321 cgtgtaggtg gttctttcta taatatacca gatatatggg atgctaaata tgcagaaagt
    13381 gctagtagta ttgttagatt attacatgat ttagatgggt ttggaggtac tgcattagga
    13441 ttaatgtttc ctgtaattgc tgcatttata ggattttcta tagctgataa attagctatt
    13501 gtaccaggtc ttgttggagg tatgattgct aaagatatag gtgcaggatt tttaggagct
    13561 ttggcagttg gtcttatagc aggatataca tgtttgttta ttaaaaatca tgttaaatta
    13621 ccaaaatcag cagcttcaat agtaccaata tttatagtac cagtatttgg gacattaatc
    13681 actgttgtat taatcaatta tgtgataggt ataccttttg ccacattaaa tactggatta
    13741 gaaaattggt taaatggatt atctggtaca aatcagatac taatggcagc tattataggg
    13801 gctatgatgg cagttgactt aggtggacca gttaataaag cagctttaac aacatcactt
    13861 gcattattaa caagtggaat ttatgcgcct aacactgctg caatggtagg aatagttata
    13921 ccaccactag gattgggact tgctactata ctagcaaaac aaaaatataa taaacagtta
    13981 cgtgaagctg gaaaatcatc tcttataatg ggtttaattg gagtaagtga aggagcaatt
    14041 ccttttgcgg tagaatctcc attaaaagta ataccttcat gtgtaattgg aacagcaatt
    14101 gcttcagcta tggcagttgg tttaggttca gtaaatgcaa ctccaattag tggattttat
    14161 ggatggttta cagtagagaa ttggccaatg tatgtgttat caatagcagt tggaacagta
    14221 attgtagctg gattagttat agtattgcgt ggaaatccag tgattgagga tgatgaattt
    14281 gaagatgatg attttgatga agcagagtgg gaatcttaaa atttaataac tatttaagac
    14341 gtgttataag ctatattcaa tatgaatctt atactaaata aagtgttaaa tattcagttt
    14401 agacttaaat ataaaagata aagaacagag gagaataaat gataaaagca catattgtta
    14461 atcatacaca ctgggataga gaatggtatt ttacttctgg agatgcatta gtacttagtg
    14521 aacaactatt tacagatgta attgatgaat tagaacgtaa tccagatgta agttttgtac
    14581 ttgatggaca actttctatt ttggatgatt atgtacaatt acacaaagaa aaaatagaaa
    14641 tcattaaaaa gttaataaaa gaagatagac tacatattgg accatggttt actcaaactg
    14701 atgcattctt tgctagagga gagtctattt taagaaactt aatgattgga atttttgaaa
    14761 gtaaaaagta tggtaagtat atgaaaattg ggtatttgcc agatacattt ggatttaatg
    14821 cacagatgcc tatactatta aagcatgtag gtcttgataa tatattcttt tggagaggta
    14881 tacatcttgg taaacaggtt caatcaccat attttaagtg gaaaagctta aatggagaaa
    14941 gttatatata tgcagtaaat atgccacatg gatatggaac tggtatgctt ttagagcctt
    15001 cattaaagta tgttaatgga cgcttagacc cagctattga ttttattaaa tcatatactg
    15061 atgtagatga ggtgttaata ccttctggga atgaccagtt aaatataata ggtgatttca
    15121 aaaataaaat taaagcgatt aatgatatcg gaaagtattc atatataacg agttcatatc
    15181 aagagttttt aaaatatata aagtccatag aatcactaga aagctataga ggtgaattta
    15241 gagaacctgt attagcaaga atacataaat caattggttc tagtagaatg gatataaaat
    15301 tagcatgtga taaactagaa aacaagctga ttaaaagaat agagccatta ttagtaattg
    15361 ctaaaaagag caaaattgaa ataagcaatc agttacttat aaatacatgg aaaaaacttt
    15421 tggaaggaca agcacacgat agtttagctg gatgtgtaac tgatactgta acagatgata
    15481 ttcttcaccg tataagagaa gcaaatgaaa tatgtgacag tattgaaaat actattgtaa
    15541 aaaagatttc agaagggctt aaattatcta aaaatgatat tttagtattt aatacagaag
    15601 ctaagcgttt taatggatat aaagagatac aagttgtaag tgatagtaaa aatatttatt
    15661 ttaaagataa tttagatgct actataattg aagaaactta tgtaaaaccg agagaaaatg
    15721 tattagaaga aacacctgct ggtaatatat ttattgaaga accaggatat tatatattaa
    15781 aagtaagaat aaatgtacag cttcctgcac ttggttataa agttgttagt tttgaattaa
    15841 gtgataaaga aatggtgtca cttgataaat ctaatgacac ttttatatct aatgataatt
    15901 ataagataat atatgaagat ggaagcttaa atttacaact taaagatgga acttacatat
    15961 caaacttttt aacattaaaa gatagtggta atgcaggtga tacctatgac ttttctccac
    16021 taaaaaatga tgagaatata aaactttcgt ttgataaagt gtatacagaa aaagctttag
    16081 gatatgaaaa aatggtggtt aaaggtacta ctttactacc acttacatta gaagatagaa
    16141 aaaacaaata tcttaatggt aaattaaatg taaaagtaag tattgtctta tctaaagaaa
    16201 acccattgat ggatgtaaaa ctttgtgttg ataacacgat atataatcat agactacgtg
    16261 ttcatataaa aactgatata aaagataata aaaacatagc atcattgcca tttggatata
    16321 taacaagaaa aaatggagtt ttagacaact gggaagatat ttacagtgaa atgcctatag
    16381 atttagaacc tttggaaagt aatattacgc ttacaaacca aaatagaagt tgtactgtat
    16441 ttactagagg aataaaagag tatcaacata ttgaagatga aattgcattg accttacttg
    16501 ctactacaga tgaactagga aagcctgatt tattatatag accaggacgt gcttctggtg
    16561 atacaactaa aaaaggtcat ataagaatta aaacagataa agctcaaatg cttaaggaat
    16621 tagaattttc atttgcaata tatatggatt ctaaatcatt tgatgaattg gaaattgcga
    16681 atttaaccca caattatcta agggaatgtg taaattatca attacaggat tacaatttct
    16741 ttttatatca tatagataat aagattcaaa aatcaattgt caataaaact gtaagtagag
    16801 aattagaaat aatatcttta ccagaaaact atcttatatc agcatgttat ccttcttatt
    16861 acagtaaaga taaatttata ataagagtgg aaaatccaag tagtaataga atgatattgg
    16921 atgagcaaat atttaaaaat agaaatggga aaattgtaaa tgcaatagaa gatgtagaag
    16981 aggaacaggt atatgaaata gcaccttttg acgttatttc aatattattg gatttataag
    17041 tgtaaaagta agtttattgt ttaaagtctg acagatatta atctgccaga ctttttaatt
    17101 tgtataaatt tgggtaaata ttaatgaatg gttttagtat ttaagtatga atttagtatt
    17161 taatatagaa tttattgaag tagaataatt agtttaacag gataagcagt ttaaataaag
    17221 aattattata ttagagtgat tttatatata atttaaaata ataaaatttt aataaaatga
    17281 gttaaagatt gtatattaat agagttgatg tagttacaaa aaagttacca attgttatgt
    17341 atttacaaaa ctgatatatt aattacataa aatctaaaat agtttgtaat aggagtatat
    17401 ataataa
//
LOCUS       DACULQ010000075        17241 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.075, whole genome shotgun sequence.
ACCESSION   DACULQ010000075 DACULQ010000000
VERSION     DACULQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.075"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(132..656)
                     /locus_tag="JA154_09960"
     CDS             complement(132..656)
                     /locus_tag="JA154_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAT7996188.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(721..1473)
                     /locus_tag="JA154_09965"
     CDS             complement(721..1473)
                     /locus_tag="JA154_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HAT7996189.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1504..2802)
                     /locus_tag="JA154_09970"
     CDS             complement(1504..2802)
                     /locus_tag="JA154_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HAT7996190.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVKSTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2819..5083)
                     /locus_tag="JA154_09975"
     CDS             complement(2819..5083)
                     /locus_tag="JA154_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HAT7996191.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5338..6159)
                     /locus_tag="JA154_09980"
     CDS             complement(5338..6159)
                     /locus_tag="JA154_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996192.1"
                     /translation="MMFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKST
                     LFMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIA
                     FAMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIV
                     LLDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILID
                     EGKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6149..6847)
                     /locus_tag="JA154_09985"
     CDS             complement(6149..6847)
                     /locus_tag="JA154_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HAT7996193.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6850..7128)
                     /locus_tag="JA154_09990"
     CDS             complement(6850..7128)
                     /locus_tag="JA154_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter substrate-binding protein
                     CbiN"
                     /protein_id="HAT7996194.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7118..7870)
                     /locus_tag="JA154_09995"
     CDS             complement(7118..7870)
                     /locus_tag="JA154_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiM"
                     /protein_id="HAT7996195.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8052..8244)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8376..10148)
                     /locus_tag="JA154_10000"
     CDS             complement(8376..10148)
                     /locus_tag="JA154_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HAT7996196.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10162..11067)
                     /locus_tag="JA154_10005"
     CDS             complement(10162..11067)
                     /locus_tag="JA154_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAT7996197.1"
                     /translation="MQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYFYGKDILSM
                     CIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTLIGTDTFID
                     NEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPGYERVNLHP
                     ATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDADEEKLIHF
                     AGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGKTFDVYEKE
                     MVGDVIKTRIPNKWESIDVFPISHI"
     gene            11320..11508
                     /locus_tag="JA154_10010"
     CDS             11320..11508
                     /locus_tag="JA154_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT7996198.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11501..12202
                     /locus_tag="JA154_10015"
     CDS             11501..12202
                     /locus_tag="JA154_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996199.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12204..13127
                     /locus_tag="JA154_10020"
     CDS             12204..13127
                     /locus_tag="JA154_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996200.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13172..13291
                     /locus_tag="JA154_10025"
     CDS             13172..13291
                     /locus_tag="JA154_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996201.1"
                     /translation="MARFWQGSCFIILIVVDFDVVSTIFCRNKFIKSEEIIYE"
     gene            13284..14207
                     /locus_tag="JA154_10030"
     CDS             13284..14207
                     /locus_tag="JA154_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996202.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14200..14937
                     /locus_tag="JA154_10035"
     CDS             14200..14937
                     /locus_tag="JA154_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996203.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            14948..15721
                     /locus_tag="JA154_10040"
     CDS             14948..15721
                     /locus_tag="JA154_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996204.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIMSLLLCACLSAFILKGLKHNEKN"
     gene            complement(16028..16240)
                     /locus_tag="JA154_10045"
     CDS             complement(16028..16240)
                     /locus_tag="JA154_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996205.1"
                     /translation="MYKVIKTVEKIEGVVNLHKHYVFVGSRRFFYCLCLWYFFLKMHH
                     VLHFVEDKFAYIEEKAVALKIKTTTL"
     gene            complement(16262..16870)
                     /locus_tag="JA154_10050"
     CDS             complement(16262..16870)
                     /locus_tag="JA154_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAT7996206.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17048..17164)
                     /gene="rrf"
                     /locus_tag="JA154_10055"
     rRNA            complement(17048..17164)
                     /gene="rrf"
                     /locus_tag="JA154_10055"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5553 a         2995 c         2179 g         6514 t
ORIGIN      
        1 cacaatacaa ataaattaaa aagtgaaatg tatttgtcta aaaacataat aacatttcac
       61 tttatcctca tttttaagta atatatattt ttaattttta tttataacta aatctagttt
      121 ttatataaat tttagttttt ttctacatct gagtaatcta ttccaaaagt ttctacagta
      181 acagatttca taatttgtgg agtttgtggc ttatcagctg aatctgttct aactgatgct
      241 atttcatcaa ctgtatccat accttctatc actctaccaa aacctgcata ctgaccatct
      301 aagtgtggtg agtttttatg cattatgaaa aattgagaac cagctgaatt aggtgccata
      361 gttctagcca ttgatatgac tcctctttca tgttttaaat tgttagtaaa tccattacca
      421 ctaaattcac cttttattga gtgacctgga ccacccattc ctgtaccatt tgggcatcct
      481 ccttgtatca taaatcctgg tattactcta tgaaatatta ttccattgta atatccttca
      541 tttactaatg atacaaagtt tttaactgta tttggagcta tatttggata taactcaatt
      601 ttgatttctt ttccattttc catttctata gttactatag gatttttatt ttccataatt
      661 tattaccttc cttttcatta gttaaattac ataattacat ttattatatt atactctaaa
      721 ttaaagtcta tcaagtatat tttttcctat ttcatatcct tcttgataaa tagttttaca
      781 aaactcatca tcaaagttaa gcgtgtcttt aggagcaaat tgagtattag gtctaactac
      841 tattatttta tcttcatcat atactttttt aatatcttct ggcaactcat aattatattt
      901 tgtggatatt aaaataattt tatccacatt atccccaatt aatggttcaa ttaatgcact
      961 cctaattaaa cctccatcta atttgaaacc atcataaatt ccttcttgca catctttcac
     1021 aaattgttct ttaaatccaa gagttgcgtt tggatttgat ggtaacaacc cactgtatga
     1081 aatgctgata atggcttcat tacgaggcat ttttgataca tcaacaatct tttctgatac
     1141 tactttatta tcaacttcaa cataattaac ataaaagttc atttcttcta tattactatc
     1201 atctagttcc cttaggttgt ctatagcagg agaattatcc acagtattat ttactatttt
     1261 tacatttcca ttctggatat cttttatatt tgtccacatt ttctccaagt tatcaacatt
     1321 ccctgtgtac aaataatatc catttattgc tcctattgat gtccctgaaa tcgaataaaa
     1381 tttatttatt cctccatcat acaaggcttt aactactcct gcttgataag ccccttttgc
     1441 tcctccacct tctaaacata atcctaattt catagtctca tctccaaatt gtttatctta
     1501 tatttatatt tcaaaaaact tactaaatgt tttatatata ctatagtggt cctcaacatt
     1561 tcctaattct cttatagaat gcattgctaa aattggactt cccacatcca cagatggtat
     1621 atctatatga gtagaagata ttggacctat agtacttcct cctctttcat ctgacctatt
     1681 aacaaattct tgatactcaa cattagcttc tctacatata tttttataaa tagctataga
     1741 atatccatca ctagtatacg cctgactagc atttatcttt ataaccggac cttttcccat
     1801 tattggttta ttagttgggt catgcttttc tggaatgttt ggatgaatag catgagctaa
     1861 atctgatgat atcataaaag atgaataaat agactcaaag aatccctctc tatccttacc
     1921 cattgatata caaattctct ccaatatatt taacaacatg tttgaatctg caccttgttt
     1981 agtagagcta ccaacttctt cattgtcaaa tacagccatt acatttacac catgttgacc
     2041 tttagtatta acaagagcat tcaaactagc atgtaccatt gcaagattat cttgtctccc
     2101 aatagatata aattcatcat tagcccctat taaacttcct ttttcaaatt catataaaaa
     2161 taaatcaaaa tctataatat cgtccatatt tacatctaag ttagatgcta tttgctttat
     2221 aagaaaatta tctttttcta atgtatcatt taacatccca actaaaggta acatatcttt
     2281 ttgcttatta tatgaatgac catcatttat acttctattt aaatgtattg ctagatttgg
     2341 tattatacaa attggctttt taaagtctat aagcctttca tttggtttta atatactatc
     2401 accttttaaa actactctac cagctatccc aagaggtcta tccaaccatg tacttaatat
     2461 tgctcctcca tagcattctg tatttaactt caaataagat ttttcagctt ctatttctgc
     2521 atttggttta atactaaatg taggagaatc tgaatgagag cctattattc taaatccatc
     2581 tctttctata tcttctgaat ttactacaaa agctactata gctgataaat tcttagtaac
     2641 ataatacttt ccaccaactc ttagattcca tctttgattc aaaactaatt cttcaaatcc
     2701 atttttatct aagatttcct tagtactctt gactgcatga aaagacgacg gactatcata
     2761 tatgaagtct attaagtttt tagcaaagtt tatactatcc attcctcatt tacctccatt
     2821 ataaaatatt tagtgttgta tattctttat ttattttttt aattccctgt tggtcaaaag
     2881 ctatagtaac atctgtacca attatagaaa ccactgtccc aaccccaaat ttaggatgat
     2941 gtaccttaga accaagtttt atatcatctg gattagtttc tttattagaa tcttttattg
     3001 tagcattaac tttatctgca actttagatt tgcttatagt actcatatac tttttagtat
     3061 atttatctaa catattataa cttgctttag aatagcttag ttcttttttt actttgtaca
     3121 atctttctat acattcttct ggcagttctt ccataaatct tgaagctatt gcaacatttg
     3181 ttttaccata taatgttctc ttctctgtca atgacatata tagctcttct ttagctctag
     3241 ttataccaac ataacaaagt cttctctctt cttctatttg tgaatcactc atagatttta
     3301 tagctcttga tataggaaat aacccttctt ccatacctgt taaaaacaca acagggaact
     3361 ctaaaccttt agatgtatga attgtcataa gcgaaaccct atcattgtct tcttcatcat
     3421 ttgattcaga tgtaagtgct atacttgtta ggaatgtctc taagtcttta ttttcataag
     3481 tatcatcatt actactttca aactccaaag ctatagaaat aaattctctc aaattatcaa
     3541 ttctgtcttg agcttcttca cctttgcctg ttaaatcttt ttcattttta tttcttatct
     3601 ctacaagctc gtccatatat cctgtagtgt ccaagacttt ttctataagt ttgctaacag
     3661 gataaacttc ttttatagtt ctaagagtcc ctataatatc aacaaatcca cttatacttg
     3721 cccttgcctt agtagatata tctgaatttg tttctatatc aattaatact gagtatatac
     3781 tttcctgttt taagttagct ctatcttcaa ttttttctat ggttctaagc cctatacctc
     3841 ttctaggaac atttataatc ctttttatag atatatcatc ttgtggattt tgaataactc
     3901 ttaaatacgc caacaaatcc tttatttctt ttctctcata gaattttgta ccaccatata
     3961 tattgtatgg aatttgactt ctatttaaag cgtcctctac aggtcttgcc tgtgcatttg
     4021 ctctataaag tactgcaaaa tccttataag gtctattttc ttttctagct atttgtgcaa
     4081 ttgaatcagc aatgaaatca gcctcttcta tttcactacc agttaattgt atctttataa
     4141 gttctccttc ttttttttca ctccaaagct tttttctttt tctttctata ttattagata
     4201 taactttatt agctgcatct aatatcactt gtgtagacct ataattttgt tccaacttta
     4261 caacatgtac attgtcataa tctttctcaa attcaagtat gtttcttata tctgcacctc
     4321 tccatccata aatactttgg tcatcatctc caacaacaca aatattttga tgttctcttg
     4381 ctaataattt tatcaattca tattgagctt tacttgtatc ttggtactca tctaccatta
     4441 tgtatctaaa tctacttctg taatatgcta acacttcatc attagccttg aaaagttcta
     4501 cagttttaag tattaaatca tcaaagtcaa gtgcactatt tctttttaaa cggtcttgat
     4561 atagcgcata aatatctgca atcttaacca ttctattatc tgccatattg atatctttaa
     4621 actgctttgg agtataaagt ttatcttttg ctcctgaaat tgcacttata actgcttttg
     4681 gttcaaacac tttatcactt aaatttaatt cttttagaca gtctttaacc aatgtaactt
     4741 ggtctgctga atcataaatt acaaaacttc tattgtaacc aatcttattt atatcttttc
     4801 ttagtatacg aacacagcaa gaatggaatg tacttatcca catatcctta gtttcaggac
     4861 ctatattttg ttcaactctc tctctcattt catttgcagc tttgtttgta aatgttatag
     4921 ctaatatatt tggtgcttgt acacctttat cttctataag atatgctatt cttgttgtta
     4981 ataccttagt tttacctgaa ccagcaccag ccagtataag tactggacct tcagtctttt
     5041 ctactgcttc tctttgagca ggatttaatg tatctaaatt catatttctc ctcctgttaa
     5101 cagccataaa tatattttaa tctaaaatat gaattacttc aaattataat tatgtattat
     5161 attaatagat tatttccaat atattacaat taaataacat ttttccatat aatttatttc
     5221 attgtttaat aaaaaaaata accttatgat ttggggaatc ataaggcacc tttaaggggt
     5281 ctaggttaag ggttattata tataaatcta tttttttaaa ataggtttag ggcttaatta
     5341 tttatttgta ttaagttctc tttccttcca atacttatac aaatcttctt ctttcttaaa
     5401 taagggtaaa ttcatatttc tatgtacttt tactaaccat ggggattcta ggccagcttg
     5461 tataatatta ttttcattta taaacacatt ttctgctttt ccttcatcaa tcaaaatccc
     5521 cttatccaat acatatatgt aatcacatat ttcatacatt aaattcatat catgactgga
     5581 tattactatt tttatattat ttttatttaa tccctttatt atatctacaa tagacctagt
     5641 acttactggg tctaaaccag ctgtaggttc atcaagtaat acaatttcat tttccattgc
     5701 tataactgat gctattgcca ctcttttttt ctgtccatag ctaagaaaat gtactggcct
     5761 gtctataaaa tctattccat taacagctac tagagctttg tttattctct ctttaattgt
     5821 tttttcatcc ataccaatgt ttctcattgc aaaggctata tcatcatata cttttgaata
     5881 aaaaatttgt ttttctgggt cttggaacac aatacctaca ttttttctaa ggttgtataa
     5941 acttctctta tcatatttta acttttcttc tttaaaaaga atttctccag atgtaggctt
     6001 taatataccc attaaattca taaataatgt tgattttcct gaaccatttg aacctattat
     6061 acctataaca tttccctttg aaaaatccat attaatgttt aatagtgcat tggtattttt
     6121 ttcatattga tacgttaaat tatttatttt aaacatcatt atcccctact atgtgaaatt
     6181 taccatcata caattttatg tctaatgata tacacatatc atcatacctt gtcataagtc
     6241 ttttgtacag catacttcca agaataccta aggatttata tgaagttttt aaatttatat
     6301 atccaaatct tagctcttgt gacttgtata tttctgaaaa ttcttctaaa aatatgaata
     6361 taaacctata tattaacatt gataactcga caaaagtgtc aggtaaatga agttttttaa
     6421 aaaagaatat taattgatta aaaggtgttg taagtataca aaaatacaca caagtcaaac
     6481 aagacatcgc tctaaataac acatgaatag acatgtcaat agacgccttt gatattccta
     6541 cattaaaact aaaaaattca aaactatata ataaatctgc tttattaaaa gatatattta
     6601 caagagttat tcctatacta agaaataaaa agtacattgg tattctaagt aattttaaat
     6661 aattttttaa atctattcca gccacacaaa caaccaaaat actcattaaa atcataattc
     6721 cacttaaaat aaatatattt tgtattacca tagaagcaat cagaaaaatt actcctatgg
     6781 ctactttttt gtttggattt actttactca atctatttgt atatgcatat ttatcaatta
     6841 taagcatgat taacttctct ttcctttttg aagacctaat atgtatccta ttacaccagc
     6901 ccctaaagct gcttgtgcag agaataataa actttctatt tctccacttg gtggctcata
     6961 tggactgcta aaccatggtt tgtaatttgg atttatctta gtaatttcac tttcagcttg
     7021 accatctgct cctccatatt cagcacctga gttgacaagt aatgggaata taataagagc
     7081 tactactaat aaaagtaata atatgttagt ttttgtttta gcactcatta gctttaactc
     7141 ctttatcatt attttctagt atgctttttt ctccgttttc tgtaattaaa ttatatacaa
     7201 ttacagtaag tataccttct gctattgcta ttggtatttg agtcataaag aaaattccta
     7261 aaaatttaat tgctgaagcc attaccccac ctgatgggtc tgggaaagcc agtgctaatt
     7321 gtattgaagt tattgtatat gtggctaagt ctcctattgc tgctgcaaga aatactggaa
     7381 tagcattatt tccatctttt ttctttaaag ccttaaatat taggaatgat acgattggcc
     7441 ctattatcgc cattgaaaaa gcattagctc ctaaagtagt tattcctcca tgtgctaata
     7501 acaatgcttg gaatattaaa actatagttc caagaacaaa cattacgctt ggtccaaaca
     7561 ttattgctcc aagacctaca ccagtaggat gtgaacaact tcctgttact gaaggtattt
     7621 ttagtgctga taatacaaac acaaatgctc cacataaagc taataatact ttctttttag
     7681 ggtcttgctt tactatttta cctattgatt ttaatccgaa taagaaaaat ggtataaata
     7741 taactcccca tgctatagac cattttactg gtaagtaacc ttccattata tgcattgcat
     7801 ttgatgtaac tggagttaaa atgattaaca taagtgcagc tataacccct aatttaatat
     7861 tctgtttcat cttagtatcc ctctctccct ttctaataat taaaacataa taatacatgt
     7921 ctgatttatt taataaaata aaaaagccta actcgatggt taggcttcta attttccaca
     7981 atctaaatac aaacacataa aacaaacata tctgtataat tattataaga aactttctcc
     8041 atccccgaag aaattacttt aatattttag gcaggtctcc tgacttagct tcatcctact
     8101 cctcttcctt cccatgagat ataaaaaata tcatcatagt ggctaacaaa agtttcgtct
     8161 gcatcacagt agcgggggct gtaaaggaat ttaacctttt tccctattaa tcttgcttaa
     8221 agaacctata atattatata tttaattttt aaattcttct taaattattt tctatataaa
     8281 tcattgtata ccttttctgt gaaaatttct acatttttta taaattttat tcatttttat
     8341 atttttatgt atataaattc ttttttgtaa gtttattaat ttctaattat cttctttaaa
     8401 tacattgaaa ctacatcaga aaatctagtt atatctcttg aatatataaa atcatttcca
     8461 tatattaatc tttcagcttc aagttctgat tcttttcctg taaatactcc taatactaat
     8521 atattttgct ttctagcttt tctcacttca ttggctgtat ctctcaaacc aactataccc
     8581 ctataagcct tttctcctct taatgttctc tcactacttt ttcctatctt tacatcattt
     8641 ggtcttccat cactgagaac tatcaggatt ttattttctt catctctatt tattagacca
     8701 ctacatatag attttattgc caaaccatct ctattattac cagcacaaaa atattcaaat
     8761 atattttcac tattttttat actatcatca taatctttaa atctttttaa tattgtatag
     8821 tctaaaaaac tagaaaatcc catcactcta tttggtatgc caacttctgt tagagcattt
     8881 gcaattatgt aagcttgaat tgccacattt ccctgatttc tactttgaga gccacttgca
     8941 tccaataata tatcaataac gtatttccct ttttcatttt ctatatcttt ataaaatatt
     9001 ttattattat tacttctacc aactctccaa gctctattgg ctaatatagt accacaatca
     9061 gaatatactc tacttttagc attttcttct attaaaattc ttgatagact ttcttttaat
     9121 ctctttattt gctgtctata cattctagca tgttctctaa attttcctaa attattttct
     9181 ttttgtcttg ttacatattt tagttgaaat acattattac actcacttct aagaactcca
     9241 tctgtaaagt gtattctaca accttcatga acatttctac aattttgtgt ctcaattttt
     9301 ttaatttcac ctttactcaa ataagttttt ccataataat gttctatttt tgaatatatc
     9361 ttatttgcag tgtcttcatc aacatatata actcggtctg ctgaattgct acatttagaa
     9421 tcatcaaaat cccctacacc ttctacaagc atggatgaag atattgaatc tatatcactt
     9481 tcaataatat tttcttcttc atcactataa agttcttcat acatgaaatc agcaaatgta
     9541 tcaaaattta cttctacact ttctacatta ttttcatcaa taagactatt ttcttctcca
     9601 tttaatatgt attcaaaata agttaaatat ataacatcta atgcttttaa aatctccata
     9661 atatcttgat tggtatctat atttcttata tcattaacta ttcttctagt catactatct
     9721 aatgctgggt gcttgtccat actataaagt acaaatgtgt atttaatttt atccataata
     9781 ttatcattgt gtattttggt aaaacgtgaa acaatatctt tataagcatt tcttcttatg
     9841 tcttctatcc ctggcctatc tttaatgacc ttttcttcaa ctattgtatt taatataact
     9901 tgaattagac ccagtataat atctttgttg tatccttttc ttattctatt tgtaagatat
     9961 tttttaacta tatcccaatc tacatacttt cttctagcac ctgcaattat tgcaaaatat
    10021 agacctattt cttttgaaga gtaatctttt tctgatatat ctatactttc attgtaatca
    10081 ccagctattg tccatgataa gttattagct ctattttcaa attcataatc atcatatatc
    10141 catttcataa tctcacctat attaaatatg acttattgga aacacatcta tactctccca
    10201 tttattaggt attctagttt taattacatc tccaaccatt tctttctcat atacatcaaa
    10261 agtctttcct gtaagtccca tattaattgc aagagttggt tttaatcctc ttcttatagt
    10321 cttaagagat gccataagac cacgtaaatc aatagcttta ctagatatct ctccattttg
    10381 agacttcaat tgtaaatcta aaaatattcc tgcaaagtgt attagttttt cttcatcagc
    10441 atctgaaaat tcatttttta aaatcatcat taacttatct tcttctatag gaggaatatc
    10501 tattaccata aatctagata ccagtgcttc attaagttct tttgttcctg catattcata
    10561 attcatagtc ccaataaatc ttgttgctgg atgtagattg actctctcat acccaggtac
    10621 atcaattatt cttctatagt ctaaagctga atgtaccaca actatagcat cattttttgc
    10681 catattaatt tcatcaaaca ccccaaaacc tccattaata gcacactcat aaactgagcc
    10741 tcttctaagt ttaacttcat tgtctataaa tgtatctgta cctataagtg ttgaactgtc
    10801 tgtatttata tgaaatgatg tattccactg aggtctatta aatatttccc ctagattatc
    10861 agcaagtaag ttttttcccg ttgcctttgg tcccgataat aatatatttt cttcttctaa
    10921 aatagctgct atacacatag acaagatatc tttaccataa aaataagttt tagactttgt
    10981 tactctgtat tctaaatctt tttctaatcc ataataattt ctaaaataca gtacatcttc
    11041 aataagtttt tcatctaccc cttgcaccct tagattatct attatatcca tatttttacc
    11101 ccttttatat aaatagtatt tgtttcattt cttatcacca ttttagcata aaaggatata
    11161 agctaaaatt gattttttaa tctcccccat tttaagtaaa accatgcacc attttatcac
    11221 tacaaaatta agagtactac ttactgatat ttcttgattt tattcgtcta tacgtatata
    11281 atatatactg ataaattatg aggaaaggat gattgaaata tggaatatat gtcttgccca
    11341 gaagtagcaa aaaaatgggg tatttctgaa agaagagtac aaaaactatg tgaagaaagt
    11401 cgcataccag gagtttcaaa actcgggtat atgtggctga taccaaagaa cgcagaaaag
    11461 cctgttgatg gtagaacaaa aagaagaaag gaagtacaat atgaatagcg ttttgattat
    11521 agatgatgac aaagaacttt gtgctcttat gaaaaaatgt gttgagcaag aaaatttatc
    11581 tgctattact gcacatggtg gtgcagaagg tctacattta gtcgataaaa atagacacac
    11641 actctctctt gtaattttgg acataatgat gccagatata gacggttttc aagttctgaa
    11701 aaaaattaga gaaacaagta atattccagt aattatgctt actgctaaaa atgacgagga
    11761 tgataaagtt tccggactac gtttaggtgc tgacgattac ctaacaaaac cgttcagcat
    11821 aaacgaactt atggctcgtg tcaattctct tattcgtcgg tatactatgc taaaccctgc
    11881 ttttataaca gacacagatt tcataataat aaatggcatg attattgaca agacaaaccg
    11941 aattgtttct gtcaataata ttcctgttca acttacaagc aaagaatttg atttacttgc
    12001 ttttctggct tctaataagg gacgggtgtt taccaaaaaa cagatttata cacaagtatg
    12061 ggaagaagaa tatgcttttg acgatagtaa catcatgtct ttcatcagta aattgagaaa
    12121 aaagattgaa ccagacccca accatccatt ttatattttg acagttcgtg gtgtcggtta
    12181 tcgttttaat aaggaggctt aacatgaata caagtgtcta tctgcttttg atattggctc
    12241 ttagcctgat tattatagcg taccttgttt ctgtcctgct acgtgtccga atacaactta
    12301 cacttataaa agatgcttta gaggatataa aaaatggaaa ccttaatcga cgtattttaa
    12361 cagatgaaaa cgacattaca aaacaaattt gctatgatat taacgaaata gcaatcaata
    12421 gccaatttca acttattcag caaaaacaat cagaacaagc ctacaaacag ctaatgacaa
    12481 gtctctccca tgatgtaaaa actccacttg cttctttggt cggttatttg gaagcagtag
    12541 aaagtaaaat tgttgtagga gaggaaaaag acgagtatat ccatgttgcg tctaataagg
    12601 ctcattactt aaagaatttt gtagaaaatc tatttgaatg ggtaaaattg gattctaagg
    12661 aacaagtttt tcattttgat atttttgact tgaatgaatt atcccgtaat attatatctg
    12721 attggatacc tgttttagaa agcagtcatt ttgaatatga atttgatata ccagaaatag
    12781 agtacttctt acgtatagac gcaaatgcat atacccgtat cattaataac ctattacaaa
    12841 atattattac ccatagtagt ggaaacaaaa tgacattgag aatttttgaa aacaaagagc
    12901 aagctcaaat tataataaca gataatggga aaggtatatc ttcagacaat ctcccacata
    12961 tttttgaaag gttgtaccaa tgcgaccatt cacgagcttc aaagggaaat ggtttaggct
    13021 tggcgattgc aaaggaactt attaacgctc ataagggaaa tattaccgcc aacagcactc
    13081 ccggtatggg aacagaattt acaatattgc ttcctaaagc cctgtaaaag tacgaggctt
    13141 tcttactcta aaacaagata aaagcaagat tatggcaagg ttttggcaag gttcatgttt
    13201 tataattcta attgtagttg attttgacgt tgtatcaact atcttttgta gaaacaaatt
    13261 tattaaaagt gaggaaatta tatatgaatg attttgtgat tgaaacaaag caacttacaa
    13321 aaatttatgg gaatcaaaca gtagttgaca aagtcaatct ccatgtaaaa aaaggccgaa
    13381 tatatggtct gttaggacgc aacggagctg ggaaaaccac aattatgaaa atgattttag
    13441 gattgacttc aatcacgtat ggcgaggtag atgtatttgg agagaatatt aagggacgtg
    13501 aaaaacaagt atatccccgt atcggtgcaa ttatcgaaac gccaggattt tatcctaatc
    13561 tgacaggaac ggagaacttg gagatttttg caaaactgcg tggtacagcc gcccctaatt
    13621 ctgtaaaaag agcattggaa attgtaggct taccttataa aaataaaaag ctgtttagta
    13681 aatattcact tggcatgaaa cagcgacttg gtattgccaa cgctatcctg catgacccag
    13741 aactgttagt tttagatgag ccaaccaatg gtcttgaccc tattggtatt gcggagatga
    13801 gaaagtttat tagggacttg agcgttgagc atggaaagac aattcttatt tccagtcata
    13861 ttctttcaga aatctcattg ttggctgatg atattggaat tatcgaccac ggtgttcttt
    13921 tggaagaaag tagtatgagt gaactagaaa agaaaaacag taaatatatc ctgctacaag
    13981 tttcagatat tccgaagaca tcacttattt tagagcgtca atttaacacg aaggattatt
    14041 ccgttcaaga tgactacacg ttacaccttt ataacactaa tctggatatg ggtgaaatca
    14101 ataaagcact tgtaatgcag aatgtaactg ttattagttc tcaactttgt aatgatactt
    14161 tagaggacta ctttaaaaaa atcacaggag gagagggcat tgcttaaact tattcaaaca
    14221 gaatttctta aactgcgtcg taggaaactt atttggctga tgttgttgtc tgctctcatt
    14281 atgccgtttt tcgcactact atacttcaat tattttggta aaacaggtgt tgagccaata
    14341 aagttttata aatggtctgc atttggttat acactttgga ttattttgcc tgttgttttg
    14401 ggtatatttt gcaccatgct tatacacgaa gaaaaccagt atgatatgct caaacagctt
    14461 tggattgtac ctattagtaa aatgggatac ttttttagta aatttttcgt tgttttgatt
    14521 tactctattt gttttatgct gattacagcc gttgcttctg tgttatttag tgtactttct
    14581 ggatatgtcg tatttagctg ggcaagcatt ttatatttgt tgaaaaaatg tttagaaatt
    14641 ggtgtaatca ctgcttttgt aatgttgccg attttggcaa tcgctacatc tcaaaaagga
    14701 tacattctcc caatttgtat cacattggta tatgcctttt ccggttttgt tataatgaca
    14761 ataaatatgt atttacaccc attatccagt atgggagtta ttatcatgag aaataaagac
    14821 attccaggtc ttatattttc acaggaaatt aatgtcccat tagcatttct ttgtatttgt
    14881 atttggagta ttgtatcagt attgtttgca aatattgcac taggaagaag aaagtgaggt
    14941 gtagataatg tggaaattac tttgggctga atgtcaaaaa attcgtcgtt caaaaattgt
    15001 ttggattaca gtttttgcat ccattatgat agcagtcata gtttttgcag gggggcagga
    15061 tgtatataat gacccagatt tacattatgg cttaaagagc gtttatgacg gttcacggta
    15121 tatagacaat gcaggttggt atatggacgc agtacaacct tggtcaactt tttttgttct
    15181 tcctgctgtc attgccctat taggaagcta tattatttgt cgtgaagaag aggaggacac
    15241 tattaaatcc ctgcgtctta ttcccataaa tgaaacaaat ttaaccatag caaaaatgat
    15301 tattactttt atattcagta tacttcttta tttgttactc tttactataa catttttgac
    15361 tgaatccgtt ttacattttt ccgatttatc gacaaaattg gttttaagtt gcatgaaaga
    15421 gtattttctt gatggcatag gtgtattctt tgcaatttct cccattattg ccttggtatc
    15481 gcgcatgaaa aaaggatatt ggcttgcatt agtattcaca gaagtttatt ctattgctgg
    15541 actatttgcg ggtatgtcaa atgtcttaca aacctttttt cctattgcgg caatatttaa
    15601 tatttctgga tactatataa ctactggaga aaaaattatg gggagtatca tgagtttatt
    15661 actttgtgcc tgcctttctg catttatact taaagggcta aaacacaatg aaaaaaattg
    15721 aaataggtat atcaaaatga aaaagataaa aaaactgatt attctaattg tattcttgtt
    15781 tctttttggc atgttacact gttatactga atggaaattc aacaataacc cacctaaagg
    15841 ggtaatagaa agaataaatg agtggactga taagattgga ctggattagt aacttatgtt
    15901 aattatagtg aatgtaatat tagcaagtta ttgtttcgta ttttaatact ataactttaa
    15961 atatttggtt ttaatcttcc aaacgaatat aaaaaaactg tcacagaggc aacatatttt
    16021 tgaatattta taaagtggta gttttgattt tcaaagctac cgccttttct tctatataag
    16081 caaacttatc ctcaacaaag tgtaaaacat ggtgcatttt aagaaaaaag taccacagac
    16141 acaagcaata aaagaaacgc cgagaaccta caaaaacgta gtgcttatgc agatttacga
    16201 cgccttctat tttctcaacc gtcttaatga ccttgtacaa aagaaatttt tctaatattt
    16261 tttataatct tatcttgtga tgttttttat gtataaatga cttaataaca aatccaataa
    16321 aaattatgga tattggtata attagaacga ggaatttata atcattataa tgtgacatta
    16381 ttcttaccca actctcagaa aaaatatatc ctatactgat taatgaagta ttccatatgg
    16441 ttattcccac gatagatgat attacaaaag ttataatatt ttgacctgct atacctgcta
    16501 taaaagatat gtatgttcta cataaaggga ttaatctaca tacacaaaca gaaatagaag
    16561 agtatttatt aaaataatct tgagatgcaa atattccttt ctttgccttt ggacatatct
    16621 ctattatttt atttatgata gatttacctc cccaatttcc cactgtataa caaaatatcg
    16681 aaccaagtat acctgcaatt atacttaaaa ttaaaattgt cagaaatcca aaatgatttc
    16741 ttgcagcaat tgctccacat aaaggtaaaa caacttcgct aggtaatgga aaacaagcat
    16801 actcaatcat aattaataca aatacagaga ttaatccata ttgtaaaata aaactattga
    16861 ttacagacac ttacttatct atctcctttc ttatttaaag gttttaaggg tacatgttct
    16921 aaggattctc accctatatt taaagaatca atctctttaa actattatat gtatttacac
    16981 aaagcattta taacatactt taaaaatagt accttaaaat aagaaactta ataaataaaa
    17041 aaagattacg tggctacgtc ctactctccc aggcagtttc ccaccaagta ccatcagcgc
    17101 tagagagctt aacttctgtg ttcggaatgg gaacaggtgt atcctctctg ctattgtaac
    17161 cacataatct ttatgcttaa tatttccatt aagtttttaa taagtttata gaacctagta
    17221 atactagttt ctttagagtt a
//
LOCUS       DACULQ010000076        17028 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.076, whole genome shotgun sequence.
ACCESSION   DACULQ010000076 DACULQ010000000
VERSION     DACULQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.076"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(228..1271)
                     /locus_tag="JA154_10060"
     CDS             complement(228..1271)
                     /locus_tag="JA154_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAT7996207.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(1292..1738)
                     /locus_tag="JA154_10065"
     CDS             complement(1292..1738)
                     /locus_tag="JA154_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAT7996208.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(1916..3496)
                     /locus_tag="JA154_10070"
     CDS             complement(1916..3496)
                     /locus_tag="JA154_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAT7996209.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            4295..5197
                     /locus_tag="JA154_10075"
     CDS             4295..5197
                     /locus_tag="JA154_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996210.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            5184..5939
                     /locus_tag="JA154_10080"
     CDS             5184..5939
                     /locus_tag="JA154_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996211.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELIDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            6036..7073
                     /locus_tag="JA154_10085"
     CDS             6036..7073
                     /locus_tag="JA154_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7996212.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            7882..8784
                     /locus_tag="JA154_10090"
     CDS             7882..8784
                     /locus_tag="JA154_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996213.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIISFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            8771..9526
                     /locus_tag="JA154_10095"
     CDS             8771..9526
                     /locus_tag="JA154_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996214.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            9657..10679
                     /locus_tag="JA154_10100"
     CDS             9657..10679
                     /locus_tag="JA154_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7996215.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(10843..11400)
                     /locus_tag="JA154_10105"
     CDS             complement(10843..11400)
                     /locus_tag="JA154_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAT7996216.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(11406..12482)
                     /locus_tag="JA154_10110"
     CDS             complement(11406..12482)
                     /locus_tag="JA154_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAT7996217.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(12483..13166)
                     /locus_tag="JA154_10115"
     CDS             complement(12483..13166)
                     /locus_tag="JA154_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAT7996218.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(13315..14127)
                     /locus_tag="JA154_10120"
     CDS             complement(13315..14127)
                     /locus_tag="JA154_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT7996219.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMEDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            14320..15249
                     /locus_tag="JA154_10125"
     CDS             14320..15249
                     /locus_tag="JA154_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAT7996220.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(15410..15859)
                     /locus_tag="JA154_10130"
     CDS             complement(15410..15859)
                     /locus_tag="JA154_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996221.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(16062..>17028)
                     /locus_tag="JA154_10135"
     CDS             complement(16062..>17028)
                     /locus_tag="JA154_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7996222.1"
                     /translation="KNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGLEQLIK
                     NEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMILVHGI
                     GSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKFVKNKF
                     PEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEKRIPDI
                     LINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNSKKMYD
                     LVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         5868 a         2717 c         2090 g         6353 t
ORIGIN      
        1 atttgctata tgttttatat tatattatat aacacttttt acattagtaa aggataaatt
       61 gtataaagat tctgttaatt tatttttata atttcatctt tctaataaaa acaaagaaga
      121 agacttaaaa atttataagt cctcttcttt tcaacgtgca tttttgtgct atatttaatt
      181 aattcgacct ttaatttcca tatccttcct atgataacaa aactctttta tttttttaat
      241 gattcattat catttaaaac ttcttttata tcaagtactt ctttgttatc atcactcata
      301 acttccttta ttcctttgaa tgttcctagg aaattaactg cttctgaagg taaaaccagt
      361 tttgttgact taccttcagc cattttttca agagcttcca tagattttaa tgctagaata
      421 ttattgtcta tatcagcctc tttcattgct gaaaaaacta attttaaacc ttctgctgtt
      481 gccatttgag ttctttttat catttcagct tcaccttgtg ccttagcaat agctgtctgt
      541 cttatagctt cagcttcacc ttctgccact aatatagcag attctttttc accttctgct
      601 acacgtacca tagcttcttt tttggcttct gctgttaata ttgcagattg tttttcacct
      661 tctgcttgaa gtattgcagc tgacttatta ccttctgctt gaagtattgc ctctcttctt
      721 tctctttctg ctctcatttg tttttccatt gcaacttgaa tatcctgagg aggcattata
      781 ttttttaact ctactctatt tacttttata ccccatttat ctgttgcttc atcaaggata
      841 gttctcattt ttacatttat tatatctctt gatgttaatg tttcatctaa atcaagttca
      901 ccaattatat ttcttagtgt tgtagctgtt aaattttcaa tagcagctat tggattagct
      961 atctcaaaca catatctaac tgggtctgta actttataat atacaactgt atcaatctgc
     1021 atagttacat tatctttagt tataactggt tgaggtggaa aatctattac aatttctcta
     1081 aggtcaatta catatgccat tttatctaaa aatggtatta agaaatgtac acctgtttca
     1141 gctacttttt gaaacttacc aagtctcatt attataccta ctttagattg ttttataact
     1201 cttatacaag ttaaacttat tgctacgact actactatta atacaatact taaaacaatt
     1261 ttagtaccca tactataaat cctcctttat tctattggtt tgtcacatta actgtggcgt
     1321 ctactttttt aacaactaat tttacccctt caatttttaa tacttctact gtagaacctt
     1381 tttctatctt ttcaccatct atagaaatag ccgtccatgt ttcatgttca actacaacta
     1441 gacctgtgtt atttggagaa atctcttctg taacaatacc ttttttagac atcattgctt
     1501 gtaaattagt gttagattta tagcctttat ctttttttac tattttttta gtagctacga
     1561 ctaacattgc tatagaaatt actgcaaata ttaaaatttg taagaaaata ctctctataa
     1621 agctacttaa aaatataagt attacagctc ctacactaaa ccatatcaat gttaagcttg
     1681 gtgttaacat ttctgctatt ccaaataaga ctgctactat caaccatatt aacttcatac
     1741 tatctaccct cttttcatat gaattataaa accctatata aattatatgt ttatacaatg
     1801 tttttgaata taaatttaaa ttacaaaaca ataaaataaa ttaattaatt aattaatagt
     1861 attatatcaa catactttgt taaatttgtt acaaagtagt atcaaatatc accaattata
     1921 tatccaacaa ctctttcatt gttaatgaaa tgttacttat taaatccgta gcatttaccg
     1981 tatacattgc tttgctcagt ttatctccta tatagccatg tatatatgct ccactaactg
     2041 tagcttccaa tatatccata ccttgaccta taaatgaagt aatcattcca agcaaacaat
     2101 cacccattcc accagttgcc atacagctat ttcctgtagg atttatatat gtttttttac
     2161 catctgttat gacagtttga taacctttta aaagcaccac cagattatat ttttgagcaa
     2221 attcttttgc aacatctact ctatgttctc ttatataccc aattgaatct cctgtcaacc
     2281 tggccatttc tcccaaatga ggtgtaatta caaatctatt tttccattct aaaaacttat
     2341 aacatctgtc cttcataacg ttcaatccat ctgcatctaa tactattggg caattagaat
     2401 tttctactat cttaagtaat ttatcaaatg tttgactatt atctcccata cctggaccaa
     2461 aaccaattgc atcactagaa ttaagtaatt tatttaatct ttcttcttca aaatttaaag
     2521 tcattgcttc tgtaagtcta atagatacct tttcttgcac atcttcacta ctaattaatg
     2581 ttacaagtcc actcccactc ttaacagcag attcagttgc tatgaaactt gcaccataaa
     2641 atccttttga acctgcaact atagatactt ttccaaaatc acttttaaaa ccatgcttat
     2701 ctcttcttct aatattattt tttacaaaag tcttatcagt tatgaactct tcatcatggt
     2761 attttcttgt aataaaatct ggaacaccta tcttttctac aactacatca cctataagtg
     2821 cgctagtgtc atagtttaga aaacctcttt tataaaactc aaagctaatc gttttgtttg
     2881 ctctaataca agttcctaat acatttccat tatcgctatc tatacctgaa ggaatatcta
     2941 tagaatacgt atttttgcta ttatcgttta ctatgtcaat aacttcttta aaaataccag
     3001 ttatatcacg ttttaatcca gtcccaaata tagcatctac tacaacatca cttttagata
     3061 ctctatcttt aaaaattgtt aaatttttat tttttataat attttgtttc tcaactttaa
     3121 taattttatc tacttcattt tcctctccat aatttttttc acttaaatca tttttattaa
     3181 tatttataat attaattccc atatttaaaa gtacattgta atttatttta gagcattcac
     3241 ttagtttttc aagattacct actaaaaata cgtttacttc ttttccatat gtatttagat
     3301 gtcttgctat tccaagacca tctcctccat tatttccaac accacataca actgtataac
     3361 tattatacat gtctatatcc atatttttca ttgcagatat aaccgcattt tccatcaata
     3421 caatcattgg aatccctact tcttctacac atgcagtatc aacatttctg cttaatttag
     3481 atgtacctat tagcataaat tatcactcct ataaagttaa agacttctta aatacttacc
     3541 aaaaatatct attgaaatct ttttaatccc ataaatatca taaataaaat cactaaaact
     3601 aagagttttg aaataattta aaataacaaa aaattttgaa ttaaaacaat tatttaattt
     3661 attataccat ataatttaag ttattatgtt gatttaaaac ctatttacac taattatcta
     3721 aatatctctt tatttttata atcttttatg acttatttat atttttcatt tttataaaaa
     3781 aacaataatt aagaatataa ctatattcta catgtaaaaa tttttttatt taaactcaat
     3841 tttctgataa tttgataatt tttcaaaatt tatagttgac ttttattttt tactgtatta
     3901 aactaattac tatacaaaac agaaaacaaa attttcaaaa ttattaaatt aaaaggagag
     3961 gtaaatatga agactttatt aaacaaacaa gtacgatttt ttagtagcca atttagttag
     4021 ggtttgtacc taagttaaga caaattgtca taacatagat acaagcccct taggattatg
     4081 atttttaagg attgtatcta tgttggctat agtaattgtt gttaaagttt ttattttatc
     4141 atcataatat agagccacat ggattatact gtgtggctta ttcgaatttt tgccatacag
     4201 ttatatatac tgtgtggttt ttttatatca aaatcatttt ttaaaaccaa attttaaaaa
     4261 ccatttaaaa tattttcaaa ggggtgtttt atttatgtca ggtattatat ctgtaatgac
     4321 gcaaagttta attttatcga ttatggcact gggggtttat ataacttaca aaatcttaga
     4381 ttttccagat atgtctgctg atggtagcta tacaatgggt gcttctattg tagcattttc
     4441 tcttactaat ggtatttctc ctgtagtagc aactttaatg gctatcttat gtggttgtac
     4501 tgctggtctt gttacaggga ttcttcacat taagtttaag atatctaatt tactttctgg
     4561 tattttagtt atgggaatgc tatactctat caatttaaga ataatgggaa aatcaaatat
     4621 tcctctattt agctttaaac acttattcaa tggggaaata tctcctatag ttttagcctt
     4681 agcttttgta ttcatctgta aagtcctttt agatttattc ctaaaaacag gtcttggata
     4741 cactttaaaa ggtgttggtg ataactctca aatgattaaa tctctaggta taaatatagg
     4801 ttctataaaa atactaggac ttatgatttc aaatggactt atagctttat ctggtagtct
     4861 tatggctcag ttcttgggat tctcagatgt aaatatgggt ataggaactt tagttcttgg
     4921 aattgcctct atcataattg gtattacact atttaagaaa tttactttta tcaaagatac
     4981 tactgcaata attgttggct cttttatata ccaattcact atatactttg caatgagttt
     5041 aggaatgtta tcaactgact taaaactaat aactgccata gttataatag ctttcctagc
     5101 tactggtaac ttaaatattt cactaaaaaa aaacaaatgc aaagctagta ccaaaaataa
     5161 atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag agtttcccta
     5221 atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata gaagatggtg
     5281 aatttgtaag tatcataggt agtaatggaa ctggtaaatc tactttatta aatataatat
     5341 ctgggcttat aaaagaatct tctggtcaaa ttactttaga tagtactact ataacaaatt
     5401 tagctgagta taaaagaact caaatagtaa gtagagtttt tcaagaccca agccttggaa
     5461 cttgtccttc tatgacggtt agagaaaatt tatctctagc acttaataaa gggaaattat
     5521 tgaacttaaa aaaatgtctt cgtcataaaa cgaatgactt ggaacactta cttgaaggaa
     5581 tttctttgga tttaaaaaaa tatttagata ttaaagtaca atatctttca gggggtcaaa
     5641 gacaatcact atcactaatt atgtctagct tagcaagtcc taaggtctta ttactagacg
     5701 aacatacagc agcacttgac ccaaaaacat caaatgaagt aatagagtta atagataaaa
     5761 tcgtaagaga gaaaaatata actacactta tggttactca caacttgaaa cacgctcttc
     5821 aatatggaga taggttgata atgcttcata agggtgaagt tgtattagat gtaaaagata
     5881 aagaaaaaaa agatttaaca gttgaagaaa tcttagaaaa atttgaatat gcagtataag
     5941 aaattaaaat cttagtttta atcagaaata tttataatct atcatatgaa tatatgaaca
     6001 aatataatta ttaaatttta agggaggaaa ataaaatgat aaaaagtaaa aaaatattaa
     6061 gtttaattat agcaggagta cttggtgtat caatgcttac tggatgttct caaaatgatg
     6121 gctccaatgc ttcaaatgaa aataaagaaa cagatagcaa aaaacaaaaa aatataggta
     6181 ttactcaatt agtggaacat ccatctctgg ataaagcaaa gaaaggattc atcaaagcac
     6241 ttgaagataa aggctataaa gatggagata atataaaaat agatttccaa aatgcacaaa
     6301 atgatatgcc tactacacaa agtattgcta gtaagtttgt atccgacaaa aaagatttga
     6361 tatatgctat atctactcca tctgcacaag cagcttataa tgctactaaa gatattccaa
     6421 taataatgac tgctgttaca gaccctgtag aagctggact tgttaaatct cttgaaaaac
     6481 caggtggaaa tgtttctggt acatctgatt atctttcaat tgataaaaca ttagaattgg
     6541 ttaaaacatt gactccaaaa gcaaagaaaa taggggttat atacaatact agtgaagtta
     6601 attcaaaaat ccaagttgat tctctacatg attatgctaa gaaaaataat tatgaagtag
     6661 ttgaaaaagg aatcagctct tcaagtgaag ttaaccaagc tatttctagt ttagttggta
     6721 aaatagatgt tttatatgtc cctactgaca atttaatagt ttcttctatg ccaatagttt
     6781 ctaaagttgc taatgaaaac aagattccta taatagcctc tgaagaaggt tctgtatctt
     6841 ctggtgcttt agcttgttgt ggaatagatt atgaaaaact aggttataag gctggagaac
     6901 ttgctattga agtattagaa ggaaaatctg ttggcgatat accagttact acattagatg
     6961 aaactgaaat aataattaac gaagatacac taaaagcact tgacatgcaa aagttatcag
     7021 cagataatat aaagtatata aagtcagatg aaaatgcaaa atctacaaaa taaaacttat
     7081 taatttcaaa aaataggtaa tatgaagtag gtaatatgaa aactttatat aataagtact
     7141 cttatccacc ctaacccttt gaaaattgtg cttattgtag caatttgaat attgcatata
     7201 aagttattca ttttccataa aagtcgtcta tattgacgac tgattatgaa agttatataa
     7261 ctttctaatc actacttgag ctgtccaaaa gaattttaat attctccaga cagctttttt
     7321 ttatgatatt tgattgatat acatgtctag ttattccata tttagtaaat attttgataa
     7381 aatttccaca ttcacttata acttaggata acgttttttt caaacaatac attaatttaa
     7441 aagtattctg agtatacatt atttagtatt ttaattaaat ttagcagttg acttttatta
     7501 ttgtataatt tataatttat aacaagtcga gaatacatat aattttctga ctaattaaaa
     7561 tattaaaagg gagagataat tatgaatact ttattaaaca atagaaaata ttattattat
     7621 tatagccact atttttaggg tttgtaccta cgttttaaca tagatacaga ccccttaaga
     7681 ctactaaaat tttgggattg tatctatgtt ggctatattg tttataaagt tttcatttaa
     7741 ctatataaga gccacataga ttaaactatg tggcttattc gtgtttttac cacatagttt
     7801 tgtacactat gtggtttttt tatacacaaa attagcaaat aattaaaatt ataaaaatat
     7861 tttcaaagga gtgttttatt tatgtcaggt attatatctg taatgacgca aagtttaatt
     7921 ttatcgatta tggcactggg ggtttatata acttacaaaa tcttagattt tccagatatg
     7981 tctgctgatg gtagctacac aatgggtgct tctattgtag cattttctct tactaatggt
     8041 atttctcctg tagtagcaac tttaatggct atcttatgtg gttgtactgc tggtcttgtt
     8101 acagggattc ttcacattaa gtttaagata tctaacttac tttctggtat tttagttatg
     8161 ggaatgctat actctatcaa tttaagaata atgggaaaat caaatattcc tctatttagc
     8221 tttaaacact tattcaatgg ggaaatatct cctatagttt tagccttagc ttttgtattc
     8281 atctgtaaag tccttttaga tttattccta aaaacaggtc ttggatacac tttaaaaggt
     8341 gttggtgata actctcaaat gattaaatcc ctaggtataa atatcggttc tataaaaata
     8401 ctaggactta tgatttcaaa cggacttata gctttatctg gtagtcttat ggctcagttc
     8461 ttgggattct cagatgtaaa tatgggtata ggaactttag ttcttggaat tgcctctatc
     8521 ataattggta ttacactatt taagaaattt acttttatca aagatactac tgcaataatt
     8581 gttggctctt ttatatacca attcactata tactttgcaa tgagtttagg aatgttatca
     8641 actgacttaa aactaataac tgccatagtt ataatatctt tcctagctac tggtaactta
     8701 aatatttcac taaaaaaaaa caaatgcaaa gctagtacca aaaataaatc agaaaaaaga
     8761 ggtgttgtag atgttacaaa ttaaaaatct ttcaaagagc ttccctaatc catatggtga
     8821 acctaatact atctttgaaa atttatcaat cgatatagaa gatggtgaat ttgtaagtat
     8881 cataggtagt aatggaactg gtaaatctac tttattaaat ataatatccg ggcttataaa
     8941 agaatcttct ggtcaagtat tattaaataa acataacttg tctaatttag ctgaacacag
     9001 aagaactcaa atagtaagta gagtttttca agacccaagt cttggaactt gcccttctat
     9061 gactgttaga gaaaacttgt ctttagctct taacaaagga aaactattaa atctaagaaa
     9121 atgtcttcgt tacaaaagag atttcttaga aaatttattg gaaggtgtat ctcttgattt
     9181 aaaaaaatac cttgatgttc aagtaaaatt tttatcagga ggtcaaagac aatcattatc
     9241 actaattatg tcgtgcttaa caaatccatc agttttatta cttgatgaac atacagcagc
     9301 acttgaccca aaaacatcaa atgaagtaat tgaattgact aataagattg taagggaaaa
     9361 aaatataact acacttatgg ttactcacaa cttaaaacac gctcttcatt atggtgatag
     9421 attgataatg cttcataagg gtgaagttgt attagatgta aaaggtaaag aaaaagaaca
     9481 attaacagtt gaagaaatct tagaaaaatt tgaatatgca gtttaaaata atatatctat
     9541 tgtattaaac tcggctttaa aataaagtta aaattacaat aattatggtt actttgtaat
     9601 aattcatatg aatatatgaa catatataat aataaatttt tgagggggat ataaaaatga
     9661 ttaataaaaa aagattggca agtttgattt tagctggtgc tcttagtata tctatgctta
     9721 cagggtgttc acaaggagga gactctggta attcaaaaca agaaagtaat tctaaaggta
     9781 aagaagttaa aaaaattggt ataactcaac tggttgaaca tccagcatta gatgccacta
     9841 ggacaggttt tgtaaaagca ttagaaaaga acggatttaa agatggagaa aatattgata
     9901 tagacttcca aaatgctcaa aatgacatgc ctactacaca aagtattgcg agtaaatttg
     9961 catctgataa gaaggactta atatttgcta tatctactcc atcagctcag gcagcattta
    10021 atgctactaa agatatacct atacttatta ctgctgtgag tgaccctgtt gctgctggat
    10081 tagttaaaac tcttgaaaag ccaggcacaa atgtatctgg aacatcagat tttgtttctg
    10141 tagacaaagg tttagaactt cttaaaatat ttgctcctaa ggcaaaaact ataggagtta
    10201 tgtacaatac tagtgaagta aattccaaag tccaagttga tgctttaaaa gaatatgcat
    10261 ctaaaaatgg atttaaagtt gttgaaaaag gtataactac ttcaaatgaa gttaaccaag
    10321 gtatttctag tttagttggc aaaatagacg tattatatgt tcctacagat aacttagtgg
    10381 cttcttctat gcctatagtc tctaaaatag ctactgagaa taaaatacct gttatagcag
    10441 ctgaatctgg gccagttgaa aaaggtgctc ttgcttgtca aggtatcaac tacgaaaaac
    10501 taggttacaa aacaggtgaa atggcagtta aaattttaaa tggtgaatca gtatctgata
    10561 tgcctgtggc tacatcagat gatacagata tcattgttaa tgaagatata ttaaaagctt
    10621 taggtatgga aaaaccttct aatgaaaata tttcttatgt aaagactaaa caagaataga
    10681 aagatatttt ctatatacga ttaatatgcc tgtttctata aattaattta tagaaacagg
    10741 catatatttt tattttaatg tattatctat ttaaagatta tatgtaaatg ctcttaattt
    10801 gtttgtataa attttacttg tataaacttt atacaatcat atttattctt ctattttctt
    10861 tattactcta catggatttc caacagctac agtatttggt ggtatatctt ttgtgactac
    10921 acttcctgca cctattacta cattatcacc tatagtaatt cctggtgtta tgattactcc
    10981 cccacctatc catacattat cacctatttt tacaggagaa ccatactcta tacctgaatt
    11041 tctaagttgt gcatcaattg gatgataggc tgtatagatt tgaacgtttg gagctaacat
    11101 tacattgtca cctatttcaa ttttacatac atctaaaaaa atacaatcat aatttgcaaa
    11161 gaaattttcg cctacatgta tgttatatcc ataatcacac ctaatatttt gttctacatt
    11221 tatttgcttg ccaactgaac caaatagttg tcttaaaata tcttctcttt tttcatattc
    11281 atcctctaaa gtattattaa acaatctagt taatttttta caatactctc tttcttttac
    11341 taacaattca tcatttgcat agtatccttt cccagacaac attttttctt tttcagtcat
    11401 atagtttact cctttttgtt ataattgtat ctaaagcaaa ataaatattc ctgtggaaaa
    11461 tctaaatata ttctgtcacc tttcttaaat ctcattttac ttttttccaa ttctatttga
    11521 ataggtacac attcgttaga taaatcagtt tttctaacat atatagtata tgtaaaagga
    11581 ttttctacaa tgttaatcaa ttcgaaacat aacttttcat ttaaatcttc attagaatcc
    11641 aagaccttaa tatgatgctc cctgattccc acatattcta tattatcacc tatttctcta
    11701 tttaatgtca actcgcatcc ccagtctgta gcataaatag tatttttatc cagtctattt
    11761 aatttggata tatttttaca ccctgttatc ttagcttcta taaggtactt tggagattca
    11821 aagagctctt ttttaggtct ctttggaaga ccttctcctt tgttgtacac tataatatca
    11881 tcacaaactc ggtaagcttc ttcaatatca tgagtaacaa atagtatatc tcccttgtaa
    11941 tcttttaaca tattcataag ttctttttcc atattgcttc ttaaatgatg gtccaatgct
    12001 gagaaaggtt catctaaaag taatatatct ggagatgttg ccaatgctct agctagtgct
    12061 accctttgtt gttgaccacc agataattgc caagggtatc tcccctcaaa cccatttaat
    12121 ttaagtatat ctaagtacct tgctactatt tccttttttt cactcttact tagttttaaa
    12181 agcccaagtt ctatattttg acttacagtc atatgtggaa acaaagcata gttttgaaat
    12241 aaaaatccta cctttctatc ttgtgttgat aaatttatct tcttttcaga atcaaataga
    12301 actctatcat ttaatacaat tttacctctt gttggtttct caagccctgc tatacatttt
    12361 aatgtcatac tttttccaga accagattct cccaaaaacc ccagagtacc tttttcttgt
    12421 ttaatttcta cctttagttt aaaagaggat aaatcttttt caatatccac gtataagctc
    12481 aactatccac acctctttcc tataattttc tgctgaaatt ccgaccaata atttagtaat
    12541 aaaatcattg ctatagatat tgctacaata atcataaccc acatcatagc tttattcata
    12601 tctccgcctt caacagcaaa aaatatagct attggcatag tttgagtttt tccaggtata
    12661 tttccagcta tcataagtgt agcaccaaat tctccaagag ctctggcaaa cgacagcacc
    12721 aatccaccaa ttattccagg ccatgctaat ggaactgcaa tcttaaaaaa tactttccat
    12781 tcattaagac ctaatgttct agcagctgac aacatattat tatcaatctg ttcaaaagct
    12841 gaccttgaag ttctatacat cataggaaaa gacaccacta ctgctgaaat tacagttgct
    12901 gtccaactaa atattaaagt cctatcgaaa tttaataaaa actttcctat aaatccattc
    12961 ttcccacata gtaataataa aaagaaacct acaactgttg gtggcaaaat tagtggaagt
    13021 gtaaatagtc catctataag acccttccac ttacctctat agttagccat aatatatgac
    13081 acaaatattc ctattacaaa agttattatt gttgacaaaa tagatgtctt aagtgatatc
    13141 cataagggtg accaatctgt tcccatcttt ccctccttaa ttttaaatgg aaatagtgaa
    13201 actaataact ctattaattt cactatttcc attttattat aatacacaat tattttcaat
    13261 tttcatttat ttatatataa tttatactta attacacata atttatttct aagtttattc
    13321 tactttttta tatccaaatt cttcaaaaat cttttgtcct gactcactta ataaaaattc
    13381 ttcaaatttc ttagcagcat ctacgttttt actagcttta attactgaaa ctggatatgt
    13441 aataggcgaa tgagtttttt cgtctgtttt ttcaactact tttatcttat cattattgac
    13501 agtatcactg aagtaaacga atccaacatc agcatttcct gattgcaccc atgctaaaac
    13561 ttcttttaca tcttttgcaa atactagctt atcttttaac ttatctttta aatttaaatt
    13621 agttaacacc tcatctgcat acttaccagc tggaacactt tcagcttcac caacagcaat
    13681 tttcttcact ttatctgtag tcaaatcttc catacctgat acactactgt cagcagaaga
    13741 aataagaact aaatcatttt ttactaaatc tttcatagta tcagaaacta ataacttctc
    13801 ttcatccaaa gctttcattt gtttttgacc tgctgaaata aataaatcac aaggtgctcc
    13861 ttgttctatt tgttgttgta atgagcctga tgctccatag ttaactgtaa gttttacatt
    13921 tgaatcaact tttttatatt cttcttcaat ctttgccatt gcttctttta aacttgcagc
    13981 agccgatata tttaactcta ctgaatcact acttttagtt gttttttctt gttttccatc
    14041 atcttttttg tcttcattac tattgcaccc aactgtcccc aaagttaaac atgcaactaa
    14101 tcctaatatt cccaatattt ttttcataac aatctccctt tttgtaaatt tgatacacat
    14161 tttaacatgt tttattatgt tttatcatgt tttgttatat atagtttaat tatatataca
    14221 tgataataaa ttttcaagta atttatttac actactttta aatattttgc tacattttcc
    14281 tttttttaat gttaaaataa ccttatatgg aggtggatta tggactcatt atcatctttg
    14341 acacctgtcg aggtagctga attattaaag ataacaaaaa atactgtata tgaattaatc
    14401 aaaagaggtg aacttccttc ttataaagta gggaaaaaaa taagagttga tactaaagat
    14461 gttgaagaat atatcaacag ccaaaaaact ggtaaagtaa aggatacaca tttcaacaat
    14521 aaaaatatag atataaataa taatttcaaa attattattt ctggtcaaga tgtaatatta
    14581 gacattttag ctaggtctgt tgaaaaaaaa ttggatggaa taagtacatt tcgctcttat
    14641 ataggtagtt ataatgcttt gtatgagtta tataatggca gagtttctat agcatcatcc
    14701 catctatggg atttcgaaac ggatgaatat aatagcactt ttgttaaaaa gctattgcca
    14761 ggaataccat gtgtacttat aaatctagct tatagaatgc aaggtttcta tgttgctaaa
    14821 ggaaatccta aagagataac aacctgggag gatttaacaa aaccaagtat aaccattgta
    14881 aatagggaaa aaggttctgg aactagggtt ttactagatg gtaaacttag acttctaaac
    14941 tttaatggta aacatataaa aggttatgat aatgaagaat tatcacatct tggagttgca
    15001 agtactgttt ctcgtggaat agcagatgtt ggtttgggaa atgaaaaagc tgctttacaa
    15061 gtaaataata tcgactttat tcctcttcac aaagagcgct atgatttagt tattaaaaaa
    15121 gaagacttac aaaatccagt ttaccaaact ataattaaca taataaactc tcctgagttt
    15181 aaagctgaac ttcaaggtct tggcggatat gatttaactg atactggaaa aactatagca
    15241 aaagtttaag attaaaatcg caatctattt tataagattt ataatttttc acaaaattta
    15301 taacttaaac acgaaataaa ttgcgatttt agtgtctcaa aaaatttatg tctttcattt
    15361 tatcatccaa tacaaaatat aatataatta attgtgcaat catattatat taatatatat
    15421 ttacataata gtatttttct ttaatgtaga ttaacatatg atatgtttta aacatcaatc
    15481 cccttgcttt cttatatata tttggcaaca catatgagtt taataaatta tatacaatgt
    15541 tgctttcttc ttgtattttt tcaaccaaca agttatttat aaaattacct ttctcatttt
    15601 caataatctc actccaattt atcttaactt ttaatatata tcttcccact aggtatacac
    15661 actctacaat aaataataaa aatacaatgc agtcagtacc tgttgtctta tataaattac
    15721 ccgatactaa tggctttgcg aagaaattat aaaaattcaa agtatcaccc gtataatcaa
    15781 aaagtattaa tagcactcct agaactctca atactttaat attcaacatg tttttatctt
    15841 tattattttt gtttttcata tatactacct ctcttttctt tagatttgat tgtaatctaa
    15901 tactaactct tttatttgag gtataaatga atttaattgg tagttatttt catttttact
    15961 agaaattaat tcacattact aaacaaaact tatatttaaa cataaaaata gcctatctat
    16021 cttttaataa atacaaaaat aaataagcta ctaatattca attaactttt atcacttaaa
    16081 acattactat ctataaattt aaatatttta ttgaagtata ctgtactatc atgttcaaat
    16141 ccatcaacat gaccaatacc ttctataagc cataggtctt ttttatcatg tttaactaaa
    16201 tcatacattt tcttgctatt ttgataatta caaacagtat cttttgttcc atgtataaac
    16261 agtatgggta cattagattt ctcaactgac tcatatggtt ttacatcact gtaccaaaat
    16321 ccagatttaa acttattata taaatctcca caagtcacca cataattgat taatatatct
    16381 ggtattcgtt tttctagtac tcccattcta attgcatctt tcatttcaga ataaggagaa
    16441 tccagtatat aaaaatcgac cttatcatct tttgaattta tctctgaatg catagcagct
    16501 gtccctgccc ccatggaaaa tccatgcaca cctaatctcc cttcaggaaa tttatttttt
    16561 acaaatttaa ctagactatc taaatcatac ctttcataaa gaccaaatgt gtaattatct
    16621 ccaccactat taccagtatt tctttgatta taaactaata cattataacc tttatctaaa
    16681 tatctatatg ctacctttaa catttcatag taactacttc caattccatg aacaagtatc
    16741 atagtatctc ttgtctttat atttgaagtt ataaaaagac tttcaagctt gtaattatta
    16801 gaactttgta tcattaattc agatgtcttg tagtttttta atttctccag tggtttatct
    16861 tttctctcag aaaatatttt tactatatct tcgtttttta ttaactgctc taaaccaact
    16921 gaactattat acactaattt tccaaatata taagatacaa ttaataggat taatagaaac
    16981 acaattgata taattataaa tttatttctt aaactaatac aattttta
//
LOCUS       DACULQ010000077        17022 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.077, whole genome shotgun sequence.
ACCESSION   DACULQ010000077 DACULQ010000000
VERSION     DACULQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.077"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(216..1106)
                     /locus_tag="JA154_10140"
     CDS             complement(216..1106)
                     /locus_tag="JA154_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAT7996223.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(1136..2401)
                     /locus_tag="JA154_10145"
     CDS             complement(1136..2401)
                     /locus_tag="JA154_10145"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088330.1"
                     /note="catalyzes the formation of L-methionine and acetate
                     from O-acetyl-L-homoserine and methanethiol; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HAT7996224.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(2511..2613)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2667..5324)
                     /locus_tag="JA154_10150"
     CDS             complement(2667..5324)
                     /locus_tag="JA154_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HAT7996225.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFAPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(5452..6195)
                     /locus_tag="JA154_10155"
     CDS             complement(5452..6195)
                     /locus_tag="JA154_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996226.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(6205..6669)
                     /locus_tag="JA154_10160"
     CDS             complement(6205..6669)
                     /locus_tag="JA154_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAT7996227.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(7021..8499)
                     /locus_tag="JA154_10165"
     CDS             complement(7021..8499)
                     /locus_tag="JA154_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAT7996228.1"
                     /translation="MHIESTMVMPIELSKKLLKSGTTTIIADPHELVNVKGVSAIDFL
                     LESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNNPRILGLGEVMCFNDV
                     INSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDHECITFDEVYEKLRAG
                     LKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDIEKQGHIRWNIKCAID
                     LGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLDKMEVDSVYKDGNLVN
                     EEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEIVPYQILTNKVYESLP
                     CADGYFVPNKEYSKLCVVERHRMTGNVAVAPLKGFGIKNGAIATTVAHDSHNIIVAGD
                     NDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFTAEEVQEITDNMLEIA
                     RKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(8659..9777)
                     /locus_tag="JA154_10170"
     CDS             complement(8659..9777)
                     /locus_tag="JA154_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT7996229.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KKHKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALRDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQYIKLPKLGMVKIRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTDVDIGAFENTNNHIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARFQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            10043..10192
                     /locus_tag="JA154_10175"
     CDS             10043..10192
                     /locus_tag="JA154_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996230.1"
                     /translation="MQAQTISYPSALSVKVYPLPLPIACSVLPSRGIVVEVSPIRSLP
                     ADCPF"
     gene            complement(10426..10602)
                     /locus_tag="JA154_10180"
                     /pseudo
     CDS             complement(10426..10602)
                     /locus_tag="JA154_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
     gene            complement(10908..12062)
                     /locus_tag="JA154_10185"
     CDS             complement(10908..12062)
                     /locus_tag="JA154_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996231.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(12307..13146)
                     /locus_tag="JA154_10190"
     CDS             complement(12307..13146)
                     /locus_tag="JA154_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAT7996232.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKKVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(13159..13755)
                     /locus_tag="JA154_10195"
     CDS             complement(13159..13755)
                     /locus_tag="JA154_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAT7996233.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(13806..14456)
                     /locus_tag="JA154_10200"
     CDS             complement(13806..14456)
                     /locus_tag="JA154_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase"
                     /protein_id="HAT7996234.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(14502..15755)
                     /locus_tag="JA154_10205"
     CDS             complement(14502..15755)
                     /locus_tag="JA154_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HAT7996235.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(15779..16420)
                     /locus_tag="JA154_10210"
     CDS             complement(15779..16420)
                     /locus_tag="JA154_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAT7996236.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            complement(16622..17008)
                     /locus_tag="JA154_10215"
     CDS             complement(16622..17008)
                     /locus_tag="JA154_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HAT7996237.1"
                     /translation="MSERKLKYPCIYKHFKGKYYATMGLSKAIDHIENICEIYGKENL
                     IQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYDDTGIFARPL
                     DMFLEKVDTDKYVNSIQEYRFEEVYK"
BASE COUNT         5285 a         3055 c         1846 g         6836 t
ORIGIN      
        1 tattgatact ataaaggttt agtctcccat tatcgcacca tatataaaat taaagccacc
       61 tctatctaca tacacctaaa gtgattttct taaaatataa aactactatt ataaggtcaa
      121 attttaaaaa aataaggtat aaagtatcat taagattaac tttataccta taaatttcta
      181 tattttttta gttaacattt atatactctt aaaatttatt gtatcaaata gttttttatc
      241 caatttctaa acaacaattc tgaatgtttt ttccatttta cagttggctc ttctgaaggg
      301 tcatcattaa tatagtaatt ttgaggtata gatattttat ctcctttatt tatatccctc
      361 ttatattctt tatcaagagt gtatttgtca tactcgctat gacccattac gaacacatct
      421 cttgaatttg taattatata gggaccagct tctttagaat gactcacaat gctcaactct
      481 ttattctctt tcaaagcttc aatatttaca gtagtatgtc ttgaatgtgg agcaaaaaat
      541 tcattttcaa atccatctac tattttacta tctttatcta ccttatgctt aaatactcca
      601 aaacattttt gacttagtgg aagttttttt acattgtagt atttgtaaag acttgcttga
      661 gctgcccaac atatatataa tgtagattta gtttttctct tagaataatc cataatttct
      721 tctagttcat tccaataatc aacttcttca aactccattt gttcaactgg agctcctgtt
      781 attataaagc catcaaaata ctctcctttg gcttcatcaa atgttttata aaattcttta
      841 acatattcat tacaacttct tttactttct cttgttgaaa ttttaataaa ttccacagac
      901 acgttgaatc ctgttttatc tatccttctt agtaaatcta attctgtatc tatctttata
      961 ggcattagat ttagtatagc tatcttagtg tctatgtttt cttcatactt taattccttt
     1021 ttaaaatctt ctttataaat tacatctatt ccttcatcta ataatttatt aataacaggt
     1081 aatccctttg gtagtattaa agccatatct tttcacttct ctttctctta gaatattaga
     1141 aatttaaagc ttgatttaaa tcagctatta aatcctctac attttctatt ccaacagata
     1201 atcttattag tgaaggcaat actccagatg caattaattg ttcctctgtt aattgtctat
     1261 gagtagttga agctggatgt ataacacaag ttcttacatc tgaaacatgt gtaaccaaag
     1321 atgctatctg taatttttcc acaaattttt tggcattttc taacccacct cttactccaa
     1381 atgataaaac accactacat cctcttgata aatacttttt ggcattctca taatacttgt
     1441 tatcttcaag tcctgggtaa ttaatccaat ctacattttc atgtttttct aaaaatctag
     1501 ctattttaag tgcattttca ctatgtctct ccattctaag atgtagtgtt tcaacattta
     1561 aattagtaag atacgcattg aatgggctta aacaatttcc atagtctcta agcaactgaa
     1621 ctcttgcttt agttgcatat gcggcatttc caaatttttg agtatagctt ataccatgat
     1681 atgttgggtc tggctcaact aattctggaa attttccatt atcccagtta aagtttccac
     1741 catccactat aattcctcca acacttgaag catgcccatc taaatatttt gtggtagaat
     1801 gagtaactat atttgctcca tactttaaag ggttacaaag cactggagat gctaatgaat
     1861 tatcaacaat aaatggaaca ttaattctct tagctacatt tgctattttt tcaaaatcta
     1921 tgacttcaag agttggattt gcaagtgttt ctgcaaacac aaccttagta ttttcctttg
     1981 caagttctac tatctcatct tcacttgaat ctaagtcaaa cgatattaaa tctataccaa
     2041 attttttaag actaggtcct agtaaattga atgttcctcc atatactttt gaagaacaaa
     2101 gtatactatc tcctgattta catatattta aaactgccaa catatttgca gattgccctg
     2161 atgatacagc tacagaagat actccaccct ctagtaaact tattttttct tcaaaagctt
     2221 gaatagtagg attgcttatt cttgaatata tatgtccatc aacttttaaa tcaaataatt
     2281 cagcaagttg gtctatactg ctatatttaa aagttgtact ttgatataaa ggtagtactc
     2341 ttggttctcc attacctggt ttataatttc cttgcacaca tattgtttct ttattataca
     2401 ttcttgtatc ccccttagat tttagtaaaa aaaattaaac tatttaccag atgagagtta
     2461 aaataaataa agcctgagta tataactcag gctctgtata agcttaatta tactcatctc
     2521 tcagctttcg ccgcaggatt tagcaccact gtatataaat atactggttg ccgggtttca
     2581 tagggccagt ccctccacct ctctggataa gatttattta atttttctat gttttatatc
     2641 ttatatattt ttcctaattt tgtcaactat ttttttaatt tttttctttt aataattttc
     2701 ccaacctcta cttgaattac agacattatg gctaaaacaa tcgttataag ccactctaag
     2761 ttatttaaaa ctgttaagtt aaaagcattt ctaaaaacag gtgcaaatag tattactgct
     2821 attaaaaatg cagaagttaa aaatgagatt aaaagaaatg gatttttacc attatttcta
     2881 ttaaacattg aatctgtatt tgaacgctga ctaaatgctc gtaacatctg tgaaaatgct
     2941 aatacacaaa atgccattgt ttgacctaca gaatgatttt ctatattaag accaatttta
     3001 tatgctaaaa tagtcattat tgccacaaat ataccttggg taacaactct ttgtataaga
     3061 tttttttcaa acaaagtggt tgatttcact ggctggtgct tcattatatt tctgcttggt
     3121 gggtcaactc caagagcaag ggctggaagc gtagcagttg caaggtttac ccatagaata
     3181 tgaacagcaa gaagcggagt atcccaattt aataatattg ctacaaacaa cgtaagtatc
     3241 tctgctatat taccaacaag caaaaattgt ataacttttt gtatgtttct atatacacgt
     3301 ctaccttctt ttattgcata agcaattgtt gtaaaactat cgtccattaa aatcatgtca
     3361 gaagaatctt ttgcaacatc tgtaccactt atacccatgg ctataccaat atcagcagac
     3421 tttaaggcag gagagtcatt tacaccatcc ccagtcattg ctggtacttc tccaattctt
     3481 tttagagatt gtataattct aagtttatca aatggggata cacgagcaaa tactgtagta
     3541 tttttaacag cttcatctag ttcatcatca ctcaaattat caagttcatc accagatatc
     3601 acagtatttt cttcactcca tattcctaac tcatgagcga ttgttgtggc agtaacttta
     3661 tggtcacctg ttatcattat ggttcttata cctgcttctt tacaagtact tactgcatca
     3721 attacctcag ttcttggtgg gtcaatcata ccaaccatac ctataaatgt taaatcatat
     3781 tctatatttt cactatcttc ttttggtata gatgaaatct cacgcttagc aaaaccaagt
     3841 acacgcaatg catctttaga catgttaaga cataattcgt gtatattctt tttatccact
     3901 tcggtaatgt ttctttcacc ttttgaagtt aatattttag tacataggtc aagaatttcg
     3961 tctactgctc ctttagtata agcaataatc ttttcatcta tattatgaac agttgtcata
     4021 cattttctat ctgaatcaaa aggctgttca aatacacgtg gatattcttc ttcaagagct
     4081 tcttgatcta ttccaaattt tttggccata aaaattaatg cgccctctgt tgggtcacct
     4141 aatatttcat ctgttttatc tggattaata catgcatcat tacaaagtgc accagcataa
     4201 acaatctctt tatacactaa tggatgtttt tcagatgcag tatctattga tgtcactttt
     4261 ccagattcaa aatctccatt gacagcaata tgtgtaacag tcattttatt taaggttaac
     4321 gtaccagttt tatcacaaca tattactgta gctcctccta atgtttcaac agcaggtaat
     4381 tttctaatta aagcatttct tttagccata cgctgtacgc ctaaagccat tacaatagta
     4441 gctgttgcag gtaatccctc tggaataatt gatatggcaa gggaaattgc aatcataaac
     4501 attggcaaga gaggtctctg ataaaatgca cctattgcaa atataacaac gcatacaata
     4561 atacctatga cacttaatgt ctttccaaca gtattaagtt tacgttttat aggagtatca
     4621 aaatcatctt gtgaatctaa aagatgtgct atgttaccca cttctgtatc cataccagtt
     4681 gctactacaa cacctacacc tctaccatat gtgactattg aagatgtata cgccatattg
     4741 ctacggtctc ctaatacaca ttctttaggt aagattgtat ctgcatcttt ttctgatgga
     4801 acagattctc ctgttaaaga agcctcttga atttttaaac ttgatgtttc tattaaacga
     4861 aggtctgctg gaaccatagc tccatcttct aaaaaaacaa tatctcctac tacaagatta
     4921 cttgctggaa taacattttc ttcaccatca cgcataacac gagctgtagg tgagttcata
     4981 ttttttaatg cttctaatga tgattctgct tttttctctt gaacaattcc aataactgca
     5041 tttactgtta caattattag tattacaaaa gcttctgtaa attcgccaaa tatcaaagat
     5101 aatattgctg ctcctattag tatcataacc atagcatctg taatttgact ccaaagcatt
     5161 tgtaaagttg ttctaggtgg tttttttcta agttcattta agccattctc tttaagccgt
     5221 ttttccgcct cggtatcggt gagcccctcc tctgatgaat ctagactttc taaaatttca
     5281 tgcgttgctc ttgaatgcca aggcatctgt ttgtgatttt ccattttgga agaccactcc
     5341 tttctttttt taatttaaga ataattatat tttaaaatca agttcaattc aatacacact
     5401 aagtatattt tacctaaatt taacaatgcc atttaatatt taacaaaact tctactcttt
     5461 tacaaatatg tattcactat tgttagataa gttttcttta tacttataac cttctaaatt
     5521 aaactttttc agttcttcaa tatctcttat cctatttttt acaatgtatt ttgtcatcat
     5581 acctctagct ttctttgctt gtgtagattc cacttttaat atgccatttt tatttacttt
     5641 aaaagtacat gtaatatatg tatcactatc tttaataaat ttttctatgg atttgctgta
     5701 ttcatttgat gaaagattaa cgatagctgt tttttctttg attagttctt tatatatacg
     5761 atttccccaa tattcgtata aattcttaaa ctcacctact cgtaattttg tctgcatttc
     5821 taatctatat tcatatatac tatcatatgg ttttacaaca ccatacaatc cacttatgat
     5881 tcgaatacag ctatttgcaa actcttcatc tttccttgta aaattttctg cttctatatt
     5941 tttatattgt attccatcat aagttaagat tgcagggttt cctaatctat caaatttaat
     6001 gctttcaaat ctatttttat ttaatacagc taatttttca ttaattttca ttttattttt
     6061 aaaatcttct ataccaaatg tctttatatt ttccactatt tcttttgtgt tatctataaa
     6121 gcaaggcaaa ctcaaatctc tatcaaatac ttctatattc ttcatatttt tagctggtga
     6181 taacattata atcataaatt cttattactc ctgtttttcc aaatattcaa gaatctcttg
     6241 tgcatttgtg tctggttttg ctttttcaaa gactttttca ataattccat tttcatctat
     6301 aatatatgtc gcacgtacaa ctcccaatgc agtttttcca tacatttttt tctctttcca
     6361 tacatcaaaa gcttttatag caactaagtc agggtcagaa agtaaaataa aaggaagttc
     6421 atgcttttct gcaaattttt gatgtgattt tatactatct ttacttatcc caataacttg
     6481 tatatcttcc tttttaaatc catcatatgc atttctaaaa gcacaagctt gacgtgtaca
     6541 tcctggtgta ttatctttag gataaaaata tactactact tttttgcctt taaaatctga
     6601 catacttacc ttatttccat ctttatcttc tagtgtaaat tctggtgctt ttgttcctat
     6661 actcaacata aatatctctc cttttgtttt tattaataat tttatactat gcttatatta
     6721 ttaccctata aatctatata taaataaaat attttatttt ttatgtataa agtttcataa
     6781 atatataatt ttcatgtatt tatatgttaa aatagactga aaatagtttt attaagatat
     6841 ctatgcttaa aacaaggtta aatgatagat taattaaaat aaggatatct aaaaataaaa
     6901 tttttagttc atgagatgct cttttttgta tgaaatcaaa tatattttta tcatttcaac
     6961 aataaaaaga gacttatccc tattttgaga aagcctcatt tattattact tttatattat
     7021 ctatataaat ttaaattcct caacatcaaa tagacctaaa tctgtaagtc ttatttgtgg
     7081 aacaactgga agtgccataa aagaaagtgt aataaaaggg tctacatact ctggaactcc
     7141 catttttctt gctatctcca acatattatc agtaatttct tgtacttctt cagctgtaaa
     7201 agtactaatt aatccagcta cttgaagact taggtgtgca agaacttttc catttgatac
     7261 aattacataa ccaccttgta tttcctttaa gtaatttata gctactagaa tatcatcatc
     7321 attatcccct gcaactataa tattatgaga atcatgggcc acagtagtag caattgctcc
     7381 attttttatg ccaaatcctt ttagtggagc aactgctaca tttccagtca ttctatgtct
     7441 ctcaacaaca caaagttttg aatactcttt atttggaaca aaatatccat ctgcacatgg
     7501 taaagattca tataccttat ttgtcaaaat ttgataaggt acaatctcca ttacataatt
     7561 tttttcactt acttttaact gtattttttc tttattaata tatttaaact tgactgtatt
     7621 aagcaattct ttatctttaa tctcataatt gtaattagaa aacatctctt cgtttaccaa
     7681 attaccatct ttatatacac tatccacttc cattttgtct aaatcattca atacaacaat
     7741 atctgcctta taacccgcac ctattgctcc aattttcctt aacccatatg cttttgcaga
     7801 attataagtt gcaactttta tagccctaac aggttccata cctaaatcaa tcgcacattt
     7861 tatattccat cttatgtgac cttgtttttc tatatcatct aagtgcttat catctgtgca
     7921 aaacataaat tcttcaattg gcaagttatg ctttagcata ccacttacta tagactttaa
     7981 attttttgct gcgcttcctt ctcttattaa aatttttaat cctgctctca atttttcata
     8041 tacttcatca aaagttatac attcatggtc attttctatt cctgcacata catatgtctg
     8101 aagtgattta ccatttatat taggtgcatg tccatctaca actttatttt taaaaagctc
     8161 tagtttatcc aagatttcat tttcagaatt tataacatca ttaaaacaca ttacctctcc
     8221 taaacctaaa attctaggat tatttacata actctccatg tcttttgcac taaatttacc
     8281 aactccatta gtttcaaaac ttgttgcagg tacggatgaa ggcaccataa tatatacatt
     8341 aagtggtata tcttttgtcg attctaataa aaaatctatt gcactaactc cttttacatt
     8401 gaccaactca tgtgggtcgg ctatgatagt tgtcgtacct gattttaata attttttcga
     8461 taattcaatt ggcattacca ttgtagattc aatatgcata tgtgcatcaa taaagccagg
     8521 tgatatatat ttattgctac actgaacctc ccatgactaa agtcacaggg ttcctgcttc
     8581 acagaatttt gcatactaaa atatgtctta ctaattctcc acaggctatc cccgtagttc
     8641 ctacggttct tacatacgtt atttattttg aattgttatt agtcttaatc cttcttttaa
     8701 tatatttata cttgcattta tatctctatc gtgagtttca ttacaactag gacaaatcca
     8761 ttctcttata tttaagtcct tcacttcctc gtttttatac ccacatttat tgcatatttg
     8821 tgaacttgca aagaatttac ctacctttac aatttgtcta ccatgccaat tagccttgta
     8881 ttctaattga cgaataaact ctgaccatga cacatcagaa attaagcgag atagtttacg
     8941 atttctaatc atattcttta cttgtaaatc ttctatgcaa ataatatcat tctcttttat
     9001 taatttagta gataattttt gtagaaaatc ctttctttga ttagctatat gttcttgaaa
     9061 tcttgcaact tttaatcttg ctttatttct atttaaacta ccgattgttt ttctcgataa
     9121 ttctctttgt aatttagcaa gtttgtttaa tgacttctta agatacttag gattttctat
     9181 aaagtctcca caacttgaga tacaaaattc tttaattcct aaatctaaac ctatatgatt
     9241 attagtattt tcaaatgctc caatatctac atcagtacag cataatgata cataatatct
     9301 accacttggt tctttagata tagtagcatt aagtattcta ccttgaggta cttgtttatc
     9361 ccttatctta accataccaa gtttaggtag ttttatatat tgaccacaat acataatgtt
     9421 tccatttgta aagttagttt tatatgaaaa tctattaatt ttctttgatt taaatttagg
     9481 aaatcctgct ttttctttaa agaatttttt atatgcatta tctaaatctc ttaaggcgtt
     9541 ttgtagtgaa aatttgtcag gttctttaag ccactttaat tcttttttta aattggtcaa
     9601 atcagaacta cactgtttat aagtaaaagt ttctttatgt tttttataaa catctattct
     9661 tttagcaaga tacttattat acacaaatct acaacatcca aaagttttgt taattaactc
     9721 ttgctgtttt ttatttggat acattctaaa cttataagct ttttccacta ctatcacctc
     9781 actttaattg ttttttgata cactttttaa tgattttatc atatttaata tattatataa
     9841 tgctgtggtg aacaatttat taatcaccta taataatgtt aacaaatgta attatttaaa
     9901 acaattattt agaaggtttt atatttaatt ttaaataaca tatttaagaa ccgtaagtct
     9961 taacttttat catttttgag gttgtcgttc acataagttc gctactacct atgcagttct
    10021 cttatgaact tcttacactt tcatgcaagc acagactata tcttatcctt cagcattatc
    10081 cgttaaggtc tacccacttc cactaccaat agcttgtagt gtacttccct caagaggaat
    10141 agtcgttgaa gtttctccta ttcggagttt acctgctgat tgcccatttt aaagtactta
    10201 gggtttaacc ttataccatc taaccaattt tttctacttt cgtaactttc acacttaggt
    10261 ttatttcatc cttatgtttt agtttgatta gctttagggg tttccagcaa ttcaagtagt
    10321 attggatagg ttctatccta tctctacata taggtttccc tatatgctga ctattttagc
    10381 tactaactca tgactaaagt cacgagtgtg cgtagctatt ttttaatcaa tttctacatt
    10441 gcccttgtaa tcgccaattc caacgatagt gtctcctact attgctacat ctcctgttat
    10501 taactcttct gtaaatacat ttatgattgt cccatttttt agtacaatgt cagctttttc
    10561 tcttccactt gaaacatcac actgtctttt taaattaccc atttttacaa ctcctcctaa
    10621 gttttttata tcaatatact gactgtatta cgtattatag tttaatttat taatatatat
    10681 tatatatgag ttttgataga aagtatcact tgaaatttgt tccataattc ctttaattaa
    10741 atagagagaa catgaggaat aatattatct cccatgttct cattaatatt taagcaataa
    10801 tttaattcat attgaatttt ttatttttat atttaatctt cactattctt ataaataaat
    10861 ttatgtgctt tattattttt ttaatacttc tttattcata attatattta acctaaaaat
    10921 gcataaacta tcatacctac aagtattgct ggtatagtag taataatcat agcccataaa
    10981 gaagattttt tatccattgc taaaagtggg aaaagtgcat ctccatcttg agaaatagag
    11041 ttggctatca ctgcaccaag tggtatagtt cccttcaaat aaaatgacat aagcacaatt
    11101 tgtatcccac aacctggaat aagtccaaga cctgcaccta taaatacact tattacacct
    11161 gttgataata ctaatttaac taagtattca tctccaccta aaattaatat tatcaaatcg
    11221 taaataagat atgcaataaa aatccatgta attacaaatg atatttctcc aacagaatgg
    11281 ataagcattt ctcgcaaaga aatcttttta ttttcttcct ctttttgatt agaattttta
    11341 aatacctttt taaaacacca catataaatt attgaaaata atattccaat cactgctatt
    11401 acttcttcca atgaatgtaa cttctcaatt ataggaagat taagtccaga gtgagcaagt
    11461 gacataaata taaaaccgat tactataatt cctatataaa ttttatatcc ctttccatgt
    11521 gttattataa atgcaagtct atttgtattt ccatgagatt tagctagagt gtctaaaatt
    11581 atttcatcat gattatgttg ttctttattt tctttagggt tatttttact tatatcatat
    11641 ttatctgatt ttctacggtt ttttaaccct aatttttctt ctagtttaaa gtaatccact
    11701 aattgtccag ttattattcc tgtaattgta ctaactatag tgacaaaaaa atacatttta
    11761 atatttgatg aaataagtac aaatgctgca tctcccatac tagctattaa acttgcaatt
    11821 attgctccaa agcttaattt tccatttata tacaatggca ttattgccaa acttccgcca
    11881 caacctggta tagctccaat taaagcacct attaagggtt gaaattttct gttttttgat
    11941 atgatattag taaaatttcc actagtttta taattaatat atccaaataa caaaataaaa
    12001 aatccaatca tagaacctac atgtaaaaac gcctcttcag aagcggatat caacatttcc
    12061 attaatttac ctcctagact aattttgcaa caattatatt atattctatt ttctattgat
    12121 aatcaatatc aatatttctt ttgaatttct aatattaaaa agtgcgcatc ttatactctt
    12181 agtttttcat cttaagatac acactttttg atattaggtt atctatttac cacatgtaat
    12241 atgaaatcaa aggtggatat tatgataaat tataacaatg tccacctatt tttattacct
    12301 atcaaattat aaattttcaa ggaatgttac aacttcttct attatccaat taggtgtaga
    12361 tgcccctgct gtaataccca aatttttata ttttttaagc gtatttacat ccaattcatc
    12421 tttagtctct attgcaaaag ttggtactaa gtcttcacaa acctttacta acttttgagt
    12481 atttgaactg tgtttacctc ctataactat catacaatca acttttttag ctaactcttt
    12541 agctgattct tgtcttgttt ttgtagctga acaaattgta tcattaaata ctatttcttc
    12601 taatttatct gatagtttat taactatact tatatacaac tctggattta ttgtagtctg
    12661 tgcaacaaga caatatcttt tggagttatc aaattccata ttttccattt gttctaaggt
    12721 ctttattatt atagctgaat tttcacacca tccatttata cctataactt ctggatgctt
    12781 catatctcca ataactatta tttcatagcc tttattatga tattcactta ctactgtatg
    12841 tattttcttt acaaatggac atgtagtatc cacaattttt aattttttat ctttggcttc
    12901 cttatatata ttttcaggaa ctccatgaga ccttattatc atattttcat gatttggtat
    12961 tgtatcaatt tcattcacag tcttaagacc tttttcttca tattttgcta ctgcttgctt
    13021 gttatgaata agaggaccta aagcatatat gccagaatca tttttttcaa cttcatccca
    13081 tgccattttc attgctcttt ttactccaaa acaaaagcca gcattttttg ctatttttat
    13141 gttcataact ttaccccctt attttgaata tttaaagtaa ttttgtttta tcacttctaa
    13201 aatctcttgt gatatccttt catggtcatc attactcaat ttttccttaa aatgttcttc
    13261 aaatgatatt ggttctccaa tatatacaat tactttacta aaaagtttat atttagatat
    13321 tattgatact ggaacaactt tagatttact ttttattgta aatacagaaa gacctgcctt
    13381 agccttacca aaagaatctc cttttattct agtaccctct ggaaatattc ctaatacata
    13441 accgtctcta actgacctta aaatagtttt tattgtagat acatctggtt tttctctatt
    13501 tataggcata acatgcaatt tttttaatat aaatcccaat ggttttactt taaacaattc
    13561 tttctttgca attgcagcta tttctctatt ttctatggca gcagccaaga atatagggtc
    13621 aagattcgat ttatgatttg atgctattac tatatttcct ctatctggaa tattctctgc
    13681 acctataact tcatatttaa aaaatatttt agaaaaaccc ttaaaaatat ttattacaaa
    13741 tctataaaaa ttcacgttta tctcccttca acactacatt tattttcatt atgatgcata
    13801 aaaaactaaa gatttatttt agatacaact tcttctacaa cttcttctat agtttttcct
    13861 gtagtatcta tttctagtgc atcattagct tttaccaatg gtgcaaattc tctatttgaa
    13921 tcaatttcat ctcgtcttat tatatcctca attacttctt ctaaagtagt attatatcct
    13981 ttattacaaa gttctttata gcgtctattt cctctttctt ctggagtagc tactaaaaag
    14041 aatttgtaat ctgcatctgg gaaaacatat gaacctatat ctctaccatc tagtattact
    14101 gaatttttca ttcctatttt tcgttgaact tcaaccatca actgtcttac ttcttttatt
    14161 tttgctacat tagaaacatt attgcttact tctattgttc ttatttcttc atttataact
    14221 tctttgtcca aatatatatt gttatcttta aaatctatat ccgaattttc agctatttga
    14281 ataacttctt tttcattatt gacatcaata ccacttttta aacacttata agtaactgct
    14341 ctgtacatag cacctgtatc tatataattt atatttagct tttttgctac tatcttggct
    14401 attgtacttt ttccagctcc tgctggacca tctacagcta taactaaatt acccatatat
    14461 acacctctat ttattcatat ttaattatta aaaaattata tttatacact tatacccgca
    14521 agatatccag ttgaaaatgc tatttgtaaa ttatatccac cagtttctgc atctatatca
    14581 attacttcac ctgtaaaaaa taagttctta actaatttag attccatagt agaagaatta
    14641 atttctttaa ctgaaatacc accagaagta accataccag tgtttatacc tgtagtccca
    14701 ttacaagtta gcttcatatt ttttatatac tcaataattc taatttcatc tgccttagac
    14761 aaatcacttg cctttttatc aactaaagat aatagagaaa aaatctcttt taagaaattt
    14821 tgaggtagta tttgcttaag attatttaga acatttttat ttggaaatgc tctgattata
    14881 ctagagattt catcagtact taaatttggt aagaaatcta tatttaattc taccttttcc
    14941 ttttcaataa tcttgttaat ataagaagac attataagaa cacaaggtcc tgttatccca
    15001 aaatgggcaa atagcatgtc tccagatttt gatattttcc ttttcttaat cttacaagat
    15061 atttccacat tcttcatgga tattccttgt aaatctttaa tccatttttc ctctattgta
    15121 agtggaacta aagcaggata tagtttgttt aatgtatgtc catgcttttt aagaatctta
    15181 tacattgaac catctgaacc cgtctttgaa taactcactc ctccagttga tataataacc
    15241 ttgtctccta gtattttatc tccattatct aaaataatcc cttttattag atactttgac
    15301 ttgtccttat tagaatcatc ttttagagca atttcttcag ttataatatc tataactttc
    15361 ttattataca ttatctttac attgtgattg agtaaatctt tatttaatac ttctattact
    15421 tctatagatt tatcattttt agtgtatact ttgtggtcat tttcttcttc aatcttatat
    15481 tccaaatttc tactttcaaa atacgaaata aggtctttat ttgtaaatgt atagaaacta
    15541 ctatagagaa attttttatt actgacaact ttatcaaaaa aatcttctat ctctctatta
    15601 ttggtaaaat tgcatctacc gccaccagtt gccctaagtt ttcttcctaa ttctccattt
    15661 ctctcaacta atattacttc attattgttt tttgaagctg acaaagctgc catcattcca
    15721 gaagctcctc caccaactac tataacttta ctcataaatt tctccttata attgattcct
    15781 actccagtgc agaattattt atctttacat gattttttat atgacttgta tcattcattt
    15841 ttactataac aggcccataa tctttaaatt ctactatatt taaagcagta ttatcttgct
    15901 taattctata tatattttcc atacctgatt ctaaaaatga caacaacata accctcaatg
    15961 taattgaatg tgtaacaaga attatatttt tattatcata tttttcattt agttccttta
    16021 taaaagcatc tactctttcc tttataatct taagagtttc gccttctggt atatttacta
    16081 agtgtggttc atttctccat gttgcatata tatctgaata atctttttga atttctttta
    16141 ttaaaaggcc ttcccaaact ccgaatccca tttctctaag tgcctcagtt ttttgaactt
    16201 caatattgag cttatctcct aaaatttgag ctgtttgcat tgctctccca aggtcgctac
    16261 taaaaatata atcaattgaa tacttgccaa tatcttcaga aagttctttt gcctgttcta
    16321 taccttgagg cgtaaggtca gaattaccat gaccttgtgt ttttcccaat atattccaat
    16381 ccgtttgtcc atgtcttact atataaaaag tatttcccat atgtcctcct aatatatgcc
    16441 tattttttat taaatataaa ttatcacttc tcaaaactta attatatcat ataatacttt
    16501 actttgtttc tattttataa tctaatctaa catattattt acaataaatc tcatttgtat
    16561 ttctgcaaat tattgtactg ttaaagaatt tttctaaatt tataaacata tttctcttat
    16621 attatttata tacttcttca aatctgtatt cttgaattga attgacatac ttatctgtat
    16681 ctactttttc taaaaacata tccaaaggtc ttgcaaatat tcctgtatca tcatataagg
    16741 tcttgtataa aactaaatca ttagtatctt cttctttttt atgataaaag tttccatcta
    16801 aatccctata aacatagata tcttcttctc tttcagtatg tctaataact aatttatatt
    16861 tatttctgtt ttggattaaa ttttctttac catatatttc acatatgttt tctatatgat
    16921 caatagcctt gctaagaccc attgtagcat aatatttccc tttgaaatgt ttatagatac
    16981 aaggatattt taactttctt tcactgatac ctaagttatt ct
//
LOCUS       DACULQ010000078        16782 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.078, whole genome shotgun sequence.
ACCESSION   DACULQ010000078 DACULQ010000000
VERSION     DACULQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.078"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            149..1537
                     /locus_tag="JA154_10220"
     CDS             149..1537
                     /locus_tag="JA154_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996238.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            1559..2518
                     /locus_tag="JA154_10225"
     CDS             1559..2518
                     /locus_tag="JA154_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT7996239.1"
                     /translation="MKMKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSIN
                     IKHCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHT
                     IMDRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHA
                     MLALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVC
                     PHCHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHG
                     DDIKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            2578..2802
                     /locus_tag="JA154_10230"
     CDS             2578..2802
                     /locus_tag="JA154_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996240.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            2795..3307
                     /locus_tag="JA154_10235"
     CDS             2795..3307
                     /locus_tag="JA154_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAT7996241.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQEDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            3339..4193
                     /locus_tag="JA154_10240"
     CDS             3339..4193
                     /locus_tag="JA154_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HAT7996242.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            4727..5521
                     /locus_tag="JA154_10245"
     CDS             4727..5521
                     /locus_tag="JA154_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7996243.1"
                     /translation="MYTIGQVSKFLGISRDTLKCYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            5625..6431
                     /locus_tag="JA154_10250"
     CDS             5625..6431
                     /locus_tag="JA154_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT7996244.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SITVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     AIHQLFNFLIGIINEDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            6493..7014
                     /locus_tag="JA154_10255"
     CDS             6493..7014
                     /locus_tag="JA154_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAT7996245.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            7429..9516
                     /locus_tag="JA154_10260"
     CDS             7429..9516
                     /locus_tag="JA154_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT7996246.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            9531..9818
                     /locus_tag="JA154_10265"
     CDS             9531..9818
                     /locus_tag="JA154_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAT7996247.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            9845..11116
                     /locus_tag="JA154_10270"
     CDS             9845..11116
                     /locus_tag="JA154_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAT7996248.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            11150..11773
                     /locus_tag="JA154_10275"
     CDS             11150..11773
                     /locus_tag="JA154_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate isomerase"
                     /protein_id="HAT7996249.1"
                     /translation="MISRRIHLMENTLSEFLDNISNELSEFIASIDETSINKACELIL
                     EAEKNHGRVHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVI
                     AISNSGETQELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKA
                     PRASILAETIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            11778..12683
                     /locus_tag="JA154_10280"
     CDS             11778..12683
                     /locus_tag="JA154_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HAT7996250.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPSKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            12789..13421
                     /locus_tag="JA154_10285"
     CDS             12789..13421
                     /locus_tag="JA154_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HAT7996251.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNIRKI"
     gene            13899..16406
                     /locus_tag="JA154_10290"
     CDS             13899..16406
                     /locus_tag="JA154_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT7996252.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
BASE COUNT         6404 a         1882 c         3063 g         5433 t
ORIGIN      
        1 ttaagtatat ctttacatta gattttataa atttattaat aagaataact tatatattcc
       61 aatgaaaacg atattggatt taagtaagtt tgtctcataa aatgaaagta tatcaattga
      121 taatacttta aataagaagg gagaaaaaat ggaagataaa ttttatgcaa aaggcaacgg
      181 aaataacgga tatattaaaa atcttgaagt ttgttccttt aataacttag atggaacttg
      241 tggaatgttt caaatggctc tgtacaaaag agatgaaaaa tactatttat atggatgctg
      301 ttttggagga aataaaaaaa atggagtaat gattagcgat attacagacc cttataatcc
      361 acaatttata aaacattttc aaatgttaga ccctaaagag tatcctacaa caacaactcc
      421 caaaattcaa atagcagatg atttaatgat agtagcaatg agttgtggaa gtggaccagg
      481 agcacttgtt gaccaagcta aattagcaaa tattaagtgt gaagcaggaa ttagaatata
      541 tagtttaaaa gaagaccctt taaatcctaa gtttttagga tattgggatt gtggcttaaa
      601 gcatgtaatg ggtgttcata gattcatgta caatggtgga agatatgtac atttatcaag
      661 tgattgtgtt ggctttgaag gtctgattta tagggtcata gatataataa atcctactaa
      721 tccagtggaa ataggtaaat ggtggagacc agaccaatat gcagatggat atccaaatag
      781 aacttttgat gcaggagcac ctcattgccc agaatttatg gataaaggat ggcttcatgg
      841 acctccattt gtaagagacg gaaaagcata ttgtggttat ggaggagctg gtttagttgt
      901 attagatgtt gaagatttaa caagaccaag atgcttaggt gaattgccat ttatgcctgc
      961 attttctagt agacttgcag gtgcaagaac tcatacagca ttaccattgc caggaagaga
     1021 tttagtcgtt gttcaaaatg agggagaaag attccagttc tttaaaccag ataacattac
     1081 agatgttcaa gctatgaata atatacatat ggttgatgtt agtgacccaa caaaaccaac
     1141 attaattgct caatttccat atcctgaagt tccaaaagat ttcccttatc ctaactttaa
     1201 tgttgcagga ttaggaaaac cagggccatt tggtccacat aatcttcatg aaccaatgga
     1261 taataaacca tggttagagc aaagaggaga tagagtatat tgctgttatt tccatgcagg
     1321 gctaagggtt tatgatgtat cagacccata ttatatcaaa gagctagcat attttatacc
     1381 accaaatcca aataaaacac cagaagaatc ttatttccca ggattcccag gaccacgctt
     1441 ggcagtaaca gaagatctta tcgttgatga tagaggctac atcatcatag atgctttaga
     1501 tgatggattc tatatattaa aaatgaaaga agattaaata aatactaaag agggagagtt
     1561 gaaaatgaaa atactaggta tttcttttgg aacaaaaaat ggaaataatg acacaatgtg
     1621 taaagttgca cttaaaggtg ccaaagaagc aggggctgaa gtagaattta ttcaaatgtc
     1681 atctattaac attaaacatt gtactggatg ttgtgcgtgt gttaaaactt tattatctgg
     1741 gaaaggtagt atgtgtgtat taaaagatga ttttgaatgg ttgcttgaca aaatgaaaga
     1801 tgcagatggt atagtagttt cagaccctat ttttgaagaa ggggcatcag gattattcca
     1861 tactataatg gaccgttttg gacccagagc agatagagga aataacatta taggaacaaa
     1921 agttgcagaa gctataggag gaaaaattcc agattcaaga atgcttaatg ataaagtaat
     1981 atcatttatg ggaataggag gttctgactg gggtacaaga gttcaatgtg aacatgctat
     2041 gctagctctc ataccaatgt ggaaagttat agataatgct tggttcccat gggcaaagga
     2101 attagtaatg gatgatacaa ggcttgttca agttcatcaa attggtttaa atatagttga
     2161 agcagcaaaa gattttgaaa aagcttcata tcaaggagaa gaaggtgtat gtccacattg
     2221 ccacaataga ttattctacc ttgagccagg tactaaaaag gcaatttgtg ctctatgtgg
     2281 tatagttgga gagttagata ctataagtgg aaaaactata tttagttttc cagaagagca
     2341 attaggtcat gctcatgata ctttaccagg taagtttata catggtgatg atattaagaa
     2401 aatggaaggt cattatgcag aagttcgtaa aacacaagaa tttaaagaaa gaaaaacatc
     2461 ttataatttt atagagacat taactaaaga aaaacaagct ggagtttcat taggataata
     2521 attaaaatac taagactctt ttttaaggga tgttagatgt tataggaggc ttattgtatg
     2581 aatgaaaagc caagttttaa acaaattgaa gttattttag cagttatttt tttggtactt
     2641 acatattaca aggttagatt aactccagca tttattgtta tatttatacc actatattta
     2701 agttattttg cagtatctat gattgtaaat caaatagcaa ttaagctatt ggagataagg
     2761 caagctaggg aagctactaa ttacaatata ggaaatggat agaaagaaaa aagaaatatt
     2821 aacactgtct atggggattg ctttactacc tcctttatgg gcagtattag caccttatat
     2881 aggaataaaa actggagctg tagcactaat ctgtgcagga ttatatgtaa ctaatggtaa
     2941 taaacaagaa gatggattaa aaataatgtt tggtttttgg tgtggagatt tatgggcagt
     3001 gttagccatt ttaattatgg gatatatgaa tttcaatcaa aacctagaat tatttttaac
     3061 acttagtata ttaggttttt ttgcagttgt aattgcatcc ttatttgaaa aaataatttt
     3121 cctaccatca tggctttgtg gttgggctat aggtctaact attatgacag gtgaaaatat
     3181 tattaattta caagatattt gtattcaaat tgctgtagct atggcagtag gagtatggta
     3241 cgtgggtgca ggtgtagatt tgtttacaat atatattttg agtaaagata ggaaaaaggg
     3301 aaattgaaag catgtgaata tactttgatt ataggaggat ggctatgggt tttgaattta
     3361 aaataatgag aagtttaata tatgtaggac ttgccaagga agaatataga cctaagctaa
     3421 tggactggtt atatcgtcac catattccag atagtattag cacttttgga ccatattgta
     3481 ctaaatatgc cttttatcaa gcatatccta caccaaatga aggtgagcgt tttggtgcac
     3541 gtaagatgca actaacagaa cattattggc ttgtagatga acatatgcct gagatggcaa
     3601 atagaattat gacagaatat atgcctatgg atgttctacg ttggcaaggg tgtataccag
     3661 atgtagaaaa taaaagggtt catgaaaatg cagaaagtgg agatgcagga cgtgcagtag
     3721 gtggagataa tggatgtcca ccatttatat ttgcctttgt tccaataaac tgggaagaag
     3781 actttagagg aaaaggacgt actgtacaag atggaccaaa ctatcgttgg caatttatga
     3841 ttaagtatcc agatggtatc tctaaagaag aaggagaaaa atggttctat gatgaggtag
     3901 tgccatactt tacaaactgt tgctatgtta atcgttttgt cagtagtaaa ataatgatta
     3961 attatggagc aactgctttt gaccgtgtgt cagaactatg gtttgaaggg gaagaagaat
     4021 ggtataaagc tgtggttgaa gagacaaagt cgtttattaa aaaaccagaa tgggcacaag
     4081 aagaggagtt cccatattta aaaccacaat tcaatatcgc atcagtattc ttaggtgata
     4141 tagcaactat ggatgcatac tcacagtatc gtggatatat accaatgaga taaaaattat
     4201 agtttgtgat aagtagagaa ggcatttact cttactggta gaagaagact tattgtatcc
     4261 ttaaaagtag gtgaatatat agttttatat ttaaatttat ataaaactaa gactaataaa
     4321 tatgaaacaa tagaattaga ataatttgat aatatttgtt aaaagagcat ttagaatgtg
     4381 tttaaagatt aagatattcc ttttatggta gcagatagtt gaaataataa aactgttatc
     4441 atgaaaggaa tatttttatg tataggaaat ttttaaaaat aaaagttaaa tatgtttaag
     4501 aacatcttaa agtgaaagaa tcattgttag aaatatgtag acaatatagt atttataacg
     4561 tatcatatta acaagtttac acttggatta tgaaataaaa agagtaattc taaaggttgt
     4621 aaatactatg ctgactttga tacttcaaag tttaggtgat tttgtaattg ttaatagaaa
     4681 tacgtttaat gagcataaag aatttatcta ataataggag aatataatgt atacaattgg
     4741 acaagtatct aaatttttag gaatatctag agatacacta aaatgttatg aagacaaagg
     4801 attagtaaat cctaagaaaa atgatgaaaa tggatataga atatataatc aggttgatat
     4861 ctatgatatt gcaacaataa acttttatag agaaattgat atagaaatta aaaagattca
     4921 agaaataaga aaaagtaaaa gtataaacaa tttagaactc ttactagaag agaaggaaca
     4981 aatcatacta aaagagatag aatataaaaa gcttctctta aaaaaggttc agttagtcaa
     5041 agaagattgt gacaaaatca aaacatattt tggaaaatac acaataaaag aaatgaagcc
     5101 tttggaagta aaaggtaaaa taaaacatgt tactaactat gaggaatatg aagccttaaa
     5161 agagaataca aacaacttaa aaaaagcagt tacattaact gatttaagaa gagtaataaa
     5221 ttttaatgaa gaaggtataa tagaagaaaa atttataatt gtaagggaag tagatgacta
     5281 caacaaaatc atagaaggtg aagttctatc tcatcccaat tgtatttata ctattattga
     5341 agatggcaga tggtcaactg gaggagaaaa tattgataat aaagtggaag acagattaag
     5401 agaaatagca atagaaaatg aatgtgaatt acttggaata gtctatgtta atatattgct
     5461 tactacttat gaagaaggga ttgagcgtat atttttagag atttatgcac ctataaaata
     5521 atataaaagc attgacctgg gacttactca taggtttaaa ctcatgatat aagcagttat
     5581 aacttatata gcaattaaat attaatttga aatgaggatt acttatgaga acagagaaat
     5641 cattaaaaag gcctatagcc agttttatta ttctaacaaa tataattttt ttaccacttt
     5701 ttttattggt agtagtgact ataatgttaa aacttccaac agtgatatct gacattgcgt
     5761 tgtgtatatc agcgtggtca tccacttttg catttatgat attatttaaa aaaatttatc
     5821 caggtaagaa atttttagta catgtaaaag atagatttag aaataaactt aaattttcaa
     5881 cagtatctat aataattagt atacaagtcc taatagctgt agtagttata tttatattag
     5941 catctaaaaa tcatggaata atgcctaact ttacagtttc atcatgggga gcatttatat
     6001 atttattttt aaaaaattta tttgcaggat cattaggtga ggaattaggt tggagaggat
     6061 ttgttcaaaa tcatcttcag aaaagacatt ctcctttaaa agcatccata acagtaggtt
     6121 tttggtgggg gatgtggcat ttaccaatat ggtttacaac aggattcaaa ggacttgatt
     6181 taataaaata tattatattt tttatgattg caattatatc aatcagtatt ataatgacaa
     6241 ctttttataa tctaaataaa actttgataa tacctattgc aatacatcaa ctatttaatt
     6301 ttttaatagg gataattaat gaagatttaa tagagcttat aaattactat gcaatgttat
     6361 atttaatatt tgcaattgca ttaatagtta taaatccagg aaatgtatta tataaaaata
     6421 ttagagagta atatatatct ttatggtaag atataaagat attattagaa gaacatccaa
     6481 ggaggaataa acatggtttt actgattgtt gatgcacaaa agttaataac aaacgagagg
     6541 ttatataaat ttaatgaatt tgtagtaaat gtagaaaatt taattgatac agctagaaaa
     6601 aataatatag aagtaatata tgtacgtcat gatgatggag ttgaaaatga attgactaaa
     6661 ggtaaaaatg gctttgaaat atacgaaaaa tttaaaccat gtaatggaga aaaaatattt
     6721 gataaaaaag taaatagtgc ttttaaagaa actggtctat tagaatacct aacaaataat
     6781 ggtgaaaaag atattattat tgctggtctc caaacagatt attgtattga tgccactata
     6841 aaatgtggat ttgaacatgg atttaatata atagttccag cttattcaaa tacaactgtt
     6901 gacaattttt ttatgtcagc agaacaatca tataaatact acaatgaatt tatatggaat
     6961 gaaagatatg cgaagtgtat ttcattagag gaaacactta gaaaaatgaa ataaaaatag
     7021 agtatgaatt gtatatgtac ataagttgat ttcataaaaa aggatgttaa attccaagtt
     7081 aaaaattaga ttaaatatat tatattatag ataaggcaca tcaattttat tttaaattaa
     7141 tgtgcttttt aattgtagta atttatttat aatcaatatg atttttatta tttaaatcaa
     7201 tttttcttga gtttctttgt ctaaagaaac tttttttatt tataaaatct acagttatat
     7261 tatagtatct attttagatg agtttaattt ttgtatcaat ctaaaacaaa agttatactt
     7321 aaatgaaatt gaaaacgata aaattctcaa ataaaataag tattgtggag gtgatgaaag
     7381 aatccaatga taatatgaag actaatctta ataagaaagt aggggttaat ggtagtggac
     7441 aaagaaattg tatcaattct ccagatttta ttaaatagca cattgattac aactaatgga
     7501 ttacaagagg agtctaattc atcaaagaga caaattactt atagaataaa taaaatcaat
     7561 gacatgctta aagtaaaaaa tgtaccactt atatgtttaa gagccgacaa agatattatc
     7621 gttagaaaag aaacaaaaaa agcaattaaa gaaattttag aaaaaaacta ttctaagaat
     7681 acttattatt ttagtaagac agaaagactt ttatatatgt atttaatgct ttttattaac
     7741 atggaggata tttcaattaa tcattttata aattctataa aagtaagtag gtctactgta
     7801 aatcttgatt tcaaggattt gataccagag cttgaaaaga agaacattaa agttaagaac
     7861 aatcgtataa atggatatta catagttggc aatgagatgg aaattaggag agtacttatt
     7921 aaaaacatta tagaaacttt gtctaatgag aaaagttcaa gagtatttga tatttttatt
     7981 aaagaatata atttagatag ttttgaagag gctaaaaaaa ttatattaaa gctaataaaa
     8041 aaatatgaaa ttacttttgt tgaagataga ttaatagaat ttatatatat atttatcttt
     8101 ctaaaagcaa ggatgtattc aactagaata gttactcagg aaaatgtaga tattcctaat
     8161 atttcagtta tgagttcaat gaaggagtat aaatttgcta aggaattatt agaagtatat
     8221 gggagtgaag ataagataaa gcctcacaat ataatgtata ttagtgcttg gattttaggt
     8281 atatcagtag gaaattatga agaagataca gaagataagg atgtaatttc aaaaattgta
     8341 aataaaatga tgacaaagtt tggatattta agcggagttt actatataag ccaagaaaaa
     8401 atatttaaac aattatattc tcattttaga cctgcctact atagactttt atttaaatta
     8461 cctatctata atccgatttg tgaaaaagta aaagaagaat ataaattagt ttatagaatt
     8521 gtaagtaatg caatgaaaga gttttgcgga ttatttggag aagaaattca ggaagaagag
     8581 ttagcatatc ttacaatgca ctttgcaaca cttttttcaa ataaaaaaga atttgatggt
     8641 tttagaaaaa gaatagcatt aatagtttgt tcaaatggcg ttggtagttc agctatttta
     8701 catgcagaac ttacaaactt atttcctgac ttacacttta taccaatgtc ggaatcagtt
     8761 ggattagaaa atataagtga acctgtagat attgtattta ctactaatta taacgcagag
     8821 ggtttaaaaa cagatgtacc agtaattaaa gtaagccctg ttatgaccac aaaagaaaaa
     8881 tataagatta cacgtgaagt gtatattcaa ttaggtgata tttttttaag acaacctaaa
     8941 atagatgagg ttatggatat aataaaaaaa tatgcaaatg taacctctga aagtattctt
     9001 tcaagtgagc tattagctta ttttacacag atagagaatt ttacatctaa ggaaggagag
     9061 gggcctatgc taagtgagat tactaatgaa acactagtaa aaataaaggt aaaagctaag
     9121 aattgggagg aagctattag acagtcagct tctgtattag ttgaaaataa caaagtaact
     9181 gaagggtatg tagatgctat gattaactca gcaaaagcat ctggaccata catagtaatt
     9241 acaaaacatg ttgcacttcc acatgctaga ccagaagctg ggtcaaaaga gattgcaata
     9301 ggcattgcta ctttagatag tcctattgag tttgggaata acgaaaacga ccctgtaaaa
     9361 tatgtatttt gtctaagtgc aatagacaat aatagccatt tacgtgctat gtctgagcta
     9421 gttgaactat tggaagaaga tgagtttttt aaggttttag atagtgctaa agaagctaaa
     9481 gaaattattg attttataaa aacacacgaa ttataaagga ggatttaatt atgtataaaa
     9541 ttttaatcgc atgtagagca ggagttggct caagtttaat gttaaaaata aagactcaac
     9601 aaattattaa ggaaaataac tttccaattg aagtagagca tggttcattg gattcactaa
     9661 atggattcaa tggagatgct gtagaaacat taattgatgt tgctgaagaa ttaaaaaata
     9721 aaaatttgaa gtttgatata attgggattc acaatattgt tgaccgtaat gaaataaaga
     9781 cagcactaga aaaattcctt gcttcaaaag agggataaat ttaagatgat aaggaggaat
     9841 atttatggag tttattatta gtttgctgag tacacctgct gttttgcttg gcgtagttgc
     9901 atgtatagga ttgattcttc aaaagaaatc agctgttgaa gtatttacag gctcttcaaa
     9961 aacacttatc ggttttttga tatttggtat tggtgcctct gctatgacag gagcacttca
    10021 aaatttcaat aaactgttcc aacatggctt tggaattact ggtgttatag cttcaccaga
    10081 agcagctaca gcacttgcac aaggtacata tggttttgca gtttcatgta cattaattct
    10141 tggattcata ttaaatttag tatttgcacg tattacaaag tttaagaata ttttctttac
    10201 aacaggtcat agtttgttct tttcttgtgt attagtttta attatgaaag cacatggatt
    10261 tgataataca atcactattt taataggtgg aactatctta gggtttatgt ctgctgcact
    10321 acctcaattc tgtcaaccat ttatgcgtga attaacaggt ggcgacgaac aagctattgg
    10381 tcactttaac atgattggtt atggtttatc aggttatatt gggcgtttat tttcaaaaca
    10441 taaagatgat acaacagaga ctatagaatt tcctaagtgg ttatcaatat tcagagactt
    10501 cttaatgggt ctatccatag ttatgttaat cttattttat attgcaacat taaaagctgg
    10561 tcaagcattt actcaagaaa ttgctggttc aacacattgg ttggtttttc cgcttataga
    10621 ggcatttaca tttgtggcag gtatgtcaat attgatgtca ggtgttcgta tgttcttagc
    10681 tgaaataact gcagcatttg taatcatttc agaaaagtat attccaggtt cacgtcctgc
    10741 gctagatgtt ccaacagtat ttccatatgc accaaatgca gttattattg ggtttatatc
    10801 agcatatgct gctggtctat tagcaattgc tattatggca tcatttccta gtatatttcc
    10861 tgtagtaatt attccagctg ctcacatatg tttcttctca ggaggtacag ctgcaatatt
    10921 tggcaatact tcaggaggat ggagaggggc tattgctggg tcatttgttg tggggttatt
    10981 acttgcattt ttaccagtta tactatatcc agtttatggc actcttggta tagaaggagc
    11041 aactttccct aatattgatt acaatgtagt tggttcgata ttacataata ttttacaatt
    11101 tataaaacct atttaaacta aattatataa aagtatagtc atagttacta tgatttcaag
    11161 gaggatacat cttatggaaa atacattatc tgaattttta gataatatat ctaatgaatt
    11221 gtcagaattt attgcctcaa tagatgagac atctataaat aaagcatgcg aattaattct
    11281 tgaagctgaa aaaaatcacg gacgtgtaca tgtgactggt attggcaaac caggacatgt
    11341 atcgggatat atttcatcat tgctttcatc tactggaacg tcagcatata tattgcatgg
    11401 tacagaagca gtacatgggt caagtggtca agtagttgaa ggagatgttg ttattgcaat
    11461 atcaaatagt ggtgaaactc aagagttaaa agcaactctt aagactctaa aagtaaatgg
    11521 agcaaagata attggtgtaa gtggtaatga gagttcattt ttaaaaaata tttctgatat
    11581 attcctgttt gcaggtgtta aacaggaagg agattgtctt aataaagctc ctcgtgcttc
    11641 aattttagct gaaacaataa tattacaatc attaagtgtt gtattacaat atgctaaagg
    11701 tcttaataca cagcagtatt tgaagtggca tccagcagga agtcttggaa agtctattag
    11761 agagggaata taattttatg aaatataaag ggattatatg tgcaatgata acaccatttg
    11821 atgaaaatca aaatattaat tcacaagcaa catgtcaatt aattgattat ttgattgaaa
    11881 agggaattta tggattattt attttgggca ctaatggtga atgtcatgta ttaacagatg
    11941 atgaaaaagt tgaatttgca aagattgtta ttaatcatac aaataatcgt gtacctgttt
    12001 ttgttggaac agggggtaat tcaactcgtg aagttgtaga tttatctaag agaatggaag
    12061 cgattggagc aagtgcatta tctattatta caccatactt tgttacacca acacaacagg
    12121 aattaatctt acattacaaa actattgcta attcagttaa tttacctatt ataatgtata
    12181 atatgccaag taagactgga attaatattg aaccagagtc agtacgtgaa ttatcaaaga
    12241 taaagaatat tgttggaatt aaggatagta gtggtaaact tgacaacatg aaagcttata
    12301 ttgaagcaac gaagaatgag aattttagtg tgttttcagg ctcagattca cttattctgg
    12361 atagtctaaa agctggtagt caaggagctg ttgcagcaac aacaaacttc ctcacagaga
    12421 ttggtatctc tatctatgaa aactttatga aaggggattt ggaagaagct caaaaagctc
    12481 aaagtagtat agaagaactt cgacgtatac tgaaacttgg aacaattcca tctgttatga
    12541 agaaaactat tgtcttgaat ggaattaatg ttggaactgc acgacttcct gtaactgaac
    12601 caactgggga agtacttgaa gaaattaaac gagtagttga aaattatagg acaatactta
    12661 ataaaagtag tgagaataga taatataaat agggaaatta cactggtata taattaatat
    12721 atatattata aaacatgact aaaattaatg aataagtaaa gtggtttgat gagtatagag
    12781 gtgataatat gaaaaagatg aatataacca aaagaatgtc agaatgtggg gctcttgcaa
    12841 ttgtaagaga agaaaatctt aaccgtgcat gtgaaattgc tgaaggatgt ataaaaggtg
    12901 gtataacagt tatagaaatg agttataccc taaataatgc tggagaaata attcaagggc
    12961 ttaataaaaa gtatggtgaa acactttgtg tgggagcagg aacagttttg gatagtgaaa
    13021 cagctcgtca tgctatatta catggagctc aatttattat tgcaccaaat tacaatgaag
    13081 gtgtagcgaa gatttgcaat agatatcaga ttccttatgc tccaggatgt acatcattga
    13141 ctgaggcagt agacgcacta agtttaggag caacttttat aaaagctttc ccaatatcag
    13201 atttctatgg acctaaacta gtaaaggtat ttaagacacc aatacctgat atgccagttt
    13261 tagcaagtgg agggattaat attgaaaacc tacatatatg gttagaaaat ggagttgatg
    13321 tatgtggatt tggtggttta ttaactaaag gttctgttga agatattgct aaaaatgcta
    13381 aaaagattcg tgaaatcatt aaaaatataa gaaaaattta acatagggct gtatcatagt
    13441 atcttataat tactatgata cagccttttt aatattattc aaattaaaaa cagtttacat
    13501 ttctattata ttacatataa tttcagtaat tttatcttaa tttttctatc tggaaaaaaa
    13561 tgatttaaaa cttaagttat gatataattt cattaaaaga aggcgatttt tagtaaatat
    13621 gagaaaataa tgtgttttta ggggaaaagt taaatacaga tttaagtgaa caagaatcga
    13681 taattaagat attaaatggt gaatagggca tcaaaaaaat tactaaatta aaagttttag
    13741 agggagaatc ttagcaagtg tgtgtgaaaa ttcaatttca aacaaaaaat aattgtaaat
    13801 gaaatcaatt tatacacgtt gaagattcac ttttatcatt atattaaaaa gcagagtgaa
    13861 ttaaaagtga gtctaaagat aggaggataa ttaaaacaat gaaagaagac attaggttac
    13921 atcgaatgtg taaaaaagaa caagcaacaa agaatgaagt tatatcagag gatattctaa
    13981 aatctatgag tattggggtc agtaaacatt ttttagatga ttcatttaca ataatatggg
    14041 gaaatacatc attctatcaa ttattaggat atacagaaga agaattttta gttaaatttt
    14101 ctagtttaaa agattattat gaacagaatc tttatcattt tgaatctatg aaaaatcatt
    14161 ttttagaggc ttatcatcat ggcgaaaaga gtatagaata tcaaatttgc atttcagcaa
    14221 ataatggaga ccctgtttgg gtaataatga ctggtatatt aattgaatca caagataatg
    14281 agaaatcaat ggtctatata ttgtattcaa aaattaatga gttaaaaata aagcaagaag
    14341 aactatctga tatggaaaaa gagagtacag agaattttaa atggatgatg tcagagtatg
    14401 ctgggaatgt ttatattagc gatatggata catatgaatt actatactta aataagcact
    14461 catgcaatac tttacaggca tcagcaaatg aacttattgg aagaaaatgt tatgaggtaa
    14521 ttcaaggtag aacttcgcca tgtccgtttt gtacaaattc gtatttaaaa aaagatgaca
    14581 tatatgaatg ggaattttat aataaaaaac tgaaacgaac atttataatt aaagaccgta
    14641 tgttgaattg gcaagggcga agagttcgta ttgaattatc ttatgatatg tatagtgctg
    14701 aatataaatt agcaaaaaaa gatcaggaaa gagaatcaat tttgaaaact attccagcag
    14761 gaatggttag attagatgca agagattatc ttactatact ttggtgtaat gatatatttt
    14821 tgaatatgat tgaatataca aaggaacagt ttgcagagga acttaataat caatgtggat
    14881 atttattttc tgaagattat aaacgtagta agaaactggc acaagatttg aaggagtctg
    14941 gggataatgt tgtatttgaa gcaaaaatat atacacgttc aagggaagaa cgtatatgga
    15001 ctgttacatt gtgttatatc agtggggaag acagttggga tggaatacca tctttttata
    15061 gtataggtat tgacataaca aaggaaagaa aacagattga gaaattacag catatatcag
    15121 agaaagatgc attaacagga atttataatc gtgctgagac tgaaaggcag attaagaaat
    15181 attttgaaaa aaatttaaat gttatgggtg cactgtttat gattgataca gataatttta
    15241 aacagattaa tgatacagaa gggcatatga ttggagatat agtacttact gagatggcat
    15301 caggtatgaa aaaaataatg cgtgacagtg atgttgttgg aagaataggt ggagatgagt
    15361 ttacaatttt tatgaagaat atttcctcag ttaaagatgc agaaaagaaa gcagaagaac
    15421 tattgtatat gttccgtcat ctatttcaga aagaaaaaag ttttctaaaa gtaacttgta
    15481 gcataggaat agctatatat ccaaaggatg gtactacatt caaggaaata tatgcaagag
    15541 ctgataaagc cctgtatcaa gcaaaaaata tgggaaaaaa taattatgta atctacaacc
    15601 aagattattt taaagaatta gaagaatttg attgttcttc tcttggtaca gttattgatt
    15661 cagaaaaaca atacgctgaa tatcctgata atcttacacg ttatatattc cgtatgcttt
    15721 atcagactga tgatatagac caggcgatta atatgatact tgaggttgtt gggaaacagt
    15781 ttgatgtaag tcgtgtttat atttttgaaa atacagaaga tggtagatat acttctaata
    15841 cttatgaatg gtgtaatgaa ggaatctctt ctcaaatggc ttatttgcaa aacagcaatt
    15901 atcaagacta tgaggattat gaaaagcttt ttgaggatga attagttttt tattgtcgag
    15961 atatccatac attaagtcct aaactagaag aattgttttc tagtcaaggt atccactcta
    16021 cacttcaatg tgcatataaa gaagatagag ttttcagtgg atttgttgga tttgatgaat
    16081 gcacaggact acgtctatgg tcacaagagg aaataagtac attatcattg atttctcaaa
    16141 ttatatcaat gtttttacaa cgtaaaaaaa ataataaact taatcaacag attcaacaat
    16201 accagaatat tttagataat attgaccagt gtatttgtgt gattaataaa gaaaataatt
    16261 ttttattata tgtaaataaa aaatttaaaa atacatattc taaattccaa attggacaac
    16321 cttattatct tgattctata gatattcata agatatccat tatatggaat aaaaaagaag
    16381 catacctatg tactagcacg atttaatggt aaagtaaagt ttatattgga cttaaattaa
    16441 atttcgtttt aaataagata ttattttagt tgcttactga aaagactaga atctatatta
    16501 atgaattata ctaattttat accactatta aaatattgtt tgctaaaaat aaaaaatagc
    16561 cttaaaacag ttgattataa aaaattaact gctttaaggc tatttttatc tttgtgacga
    16621 aatagacact tgccatttag aatgtgacaa tatagtagtt gtttagaaaa ggcacaaagc
    16681 attatacttt aactaaagta aaatgtattt tgcttttaga ataaaaacaa ttaggaggga
    16741 gctataattg aaaaagataa tatatgtatt atttacaatt ga
//
LOCUS       DACULQ010000079        16657 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.079, whole genome shotgun sequence.
ACCESSION   DACULQ010000079 DACULQ010000000
VERSION     DACULQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.079"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..129
                     /locus_tag="JA154_10295"
     CDS             <1..129
                     /locus_tag="JA154_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAT7996253.1"
                     /translation="IKNNNLDDFTYNKMLQKIIESNRVDLEKKNIIRSMKRKTRRC"
     gene            143..817
                     /locus_tag="JA154_10300"
     CDS             143..817
                     /locus_tag="JA154_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT7996254.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYIFKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            834..1679
                     /locus_tag="JA154_10305"
     CDS             834..1679
                     /locus_tag="JA154_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996255.1"
                     /translation="MLNMNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDK
                     NEIKIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKK
                     IISSDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINI
                     RNNNIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNEN
                     GEYLTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHK
                     VKIDK"
     gene            1695..2366
                     /locus_tag="JA154_10310"
     CDS             1695..2366
                     /locus_tag="JA154_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996256.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            2356..3588
                     /locus_tag="JA154_10315"
     CDS             2356..3588
                     /locus_tag="JA154_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996257.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            3738..4055
                     /locus_tag="JA154_10320"
     CDS             3738..4055
                     /locus_tag="JA154_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAT7996258.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            4063..4950
                     /locus_tag="JA154_10325"
     CDS             4063..4950
                     /locus_tag="JA154_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin reductase"
                     /protein_id="HAT7996259.1"
                     /translation="MSIMRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLV
                     KAPSIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMY
                     EATTVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEAN
                     FLSELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIE
                     VDGVFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAG
                     QGQIAALNAVYYLDKLKRA"
     gene            5220..5588
                     /locus_tag="JA154_10330"
     CDS             5220..5588
                     /locus_tag="JA154_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAT7996260.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            5591..5962
                     /locus_tag="JA154_10335"
     CDS             5591..5962
                     /locus_tag="JA154_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT7996261.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(6107..6562)
                     /locus_tag="JA154_10340"
     CDS             complement(6107..6562)
                     /locus_tag="JA154_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996262.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(6601..7866)
                     /locus_tag="JA154_10345"
     CDS             complement(6601..7866)
                     /locus_tag="JA154_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996263.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            8336..9787
                     /locus_tag="JA154_10350"
     CDS             8336..9787
                     /locus_tag="JA154_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAT7996264.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(9985..10626)
                     /locus_tag="JA154_10355"
     CDS             complement(9985..10626)
                     /locus_tag="JA154_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996265.1"
                     /translation="MTDNYIFDSYNNLVDGLSAYLGEGYEFILYKFNGVHYSINKIAN
                     GKYNTVKEGDIITDFDLALINKLENDADGDFISYFNRNKLGEPLKSTDIVIRGVDNKI
                     IGLLCIHFYLNTPLTRIIGSYIPEHNAMQSAPVLENIVEDTDEVILKSYQDAKVIVEN
                     DSNISSNDKNKSIVSILNESGIFKIKDSVVKVADLMGLSKNTIYMHLRNLNKN"
     gene            11100..12479
                     /locus_tag="JA154_10360"
     CDS             11100..12479
                     /locus_tag="JA154_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose-like IIB subunit"
                     /protein_id="HAT7996266.1"
                     /translation="MAQDKIVVEILENIGGKENIKEVAHCMTRLRIKVCDSSQVNQEK
                     LKKIKGVLGLVLKGEQYQIVIGPNVSQVYNTLNEVIGIIPEKDSKKTISEGKQNSIAK
                     ILDTIAGIFNPIVPALAGAGLIKAILALLVASKFLSADSETYIILNTISDGVFSFLPF
                     MLAYSAAKIFKMNTYVAVAIAGAMLHPNFINLIQQGLTKISFIGIPMSLIQYNGSVFP
                     IVLTIWFASYIEKGVDKITPKALKIILVPALTILFAAPIALIIIGPIAQMLGNYIAGG
                     ASILFERGGIFAGLIYGGIYSFMVVTGLHHGMVPVLVDGITRYGFNYISPVSGSSNMA
                     QAGSAFGVWLKAKDKDLKAVAATATISALTGVTEPAIYGVNLRLKKPFLCAAIGGAAG
                     GGFAAYFQCKAFAMGGPSFLTLPMFIGEGGHVGLVAIAFTIAFIVSAIATYIVGFEEE
                     VTADSHANL"
     gene            12523..13257
                     /locus_tag="JA154_10365"
     CDS             12523..13257
                     /locus_tag="JA154_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007544858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAT7996267.1"
                     /translation="MKIYLTKDYEEMSQVAAQMVLKYMYSNDNKRINLSITAGQTPKR
                     TYEILSEFVKNKPYFENVHYYNFDEIPIKGEKVGVTIRNLTNMYFREAQIPEERIHVL
                     DENNYQGFDRKIEADGGIDMVLMGLGWDGHFCGNLSGTVNSFDEETRAVSTYITDDMA
                     KLLTKSVGGNPEKLFDYYVTYGPKTIMHCKNIIMIVNGKHKAQILKQTLFGPLDLLCP
                     SSLLRLHPNFTLIVDEEAASEIKDLI"
     gene            13346..14545
                     /locus_tag="JA154_10370"
     CDS             13346..14545
                     /locus_tag="JA154_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAT7996268.1"
                     /translation="MYNHMKKVFQGLEGGLFSSVEKADVGDSYQKLGEQGVSLMGWAD
                     PFFPDYCIPRHILDAATLAISSNMSTHYTAPVGNDLLKIEIAEKLKEVNNLDVDFKRN
                     ILITPGSDSGLYYSMLPFINEGDEVLIPTPCYPNNIQNTKLMGGVPIFVELKESDGYQ
                     IDITEFEKRLTNKTKLVVLTHPNNPTTTIFNKESLEKLAKFIIDNDLILICDQAFEDF
                     TFENELITPASLEGMFERTVTIFSTSKGMGLSGFRVAYMVCSDKLMDKYYGAAVTVLG
                     ATNTVAQMSTIEAFKDRSFMEDFERVFDKRRQEAYKIINEIPNVSMQMPESGFLGWIN
                     VSKLGTSSEIQKYLVDEAKVCINDGINYGPGGEGHLRIILGSYKDDSKVIGALNRIKD
                     ALIKKQQ"
     gene            14569..15051
                     /locus_tag="JA154_10375"
     CDS             14569..15051
                     /locus_tag="JA154_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAT7996269.1"
                     /translation="MLSIFKSKKNDNKLKAFASGKVIPIEDVDDNVFSSKMLGDGIAI
                     YPTNEYVVAPCDAEVNIIMKDTKHAVGIKLFNGMEILFHVGIDTVSMNGEGFEIFVSP
                     GDKIKRGDKLISFNSKLIKEEGLQDVVIMVVTNSEQYKNIKYHIGLEAVIDDTIIAEI
                     "
     gene            complement(15369..15966)
                     /locus_tag="JA154_10380"
                     /pseudo
     CDS             complement(15369..15966)
                     /locus_tag="JA154_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(16210..16638)
                     /locus_tag="JA154_10385"
     CDS             complement(16210..16638)
                     /locus_tag="JA154_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996270.1"
                     /translation="MKIHSIQNLTLDTRNLIKKYSLTLNEDLIWEFKHGKYHTIKYFT
                     HKFAVKESVLALLFNIHKLCYAKIKYFESSFSKYEPYKYSYQNEFVKCEMYDMNFMFH
                     KYSNIMIDLRNLQEIKTIEEFRLFCDYLESFEDNLQDLII"
BASE COUNT         6483 a         1854 c         2696 g         5624 t
ORIGIN      
        1 ataaaaaata ataatttaga tgatttcact tataataaaa tgctacaaaa aataattgaa
       61 tcaaatagag tagatttaga aaagaagaat ataataagaa gtatgaaaag aaagacaaga
      121 agatgttgac ggaggaatat taatgaataa caatgaagac agaattgaca aatttttaga
      181 acttcaaagc aaaggattta aatttgcaga gatagctcaa aatctaaaga ttagtcaaag
      241 tactttaaga acttttttaa ataaaaaagg atataagtca gaaaaaggta aatacatatt
      301 taaagacgaa ttaaattata ataaaagttt ggataataga aacttggaag aagagaaagc
      361 taaggagtat gaaaaaagta aaaagaatac tataagtaat agaaaaaaag taagcaccaa
      421 aaaaaagatt tctaatgaaa aggctttaag taaaagtgtt gaacaaattg agtttaatga
      481 tacaggttta aaaaatgcaa agccaaaaca aaatattaag cctaagaaag atagaaaaat
      541 aaatataact caagaagatt tagataagct ttgtgaagta tatgattggt atttagaagt
      601 aaaagattac aatataaagc ctaaaaaagt atctaataaa aaagatgtac atatagatga
      661 tgaacacata gatgaattaa aaacaacaag aatacgtata gataaagaaa tatggaatga
      721 ttttgaaaga ttatgtagta attcgaattt tgaaaaacat caaatactta cacaggcatt
      781 gaaagaattt atgaaaaagt ataaaaatct actataaaat actactatat gaggtgctta
      841 atatgaataa aatagtaaaa aaatcaattt ctctaatgat tatattaaca atatttattt
      901 ttatgttgac agcatgtgaa aaagatgaac agcctgattc agttgatatt caaacagaag
      961 ataaaaatga gataaaaata gatgagggaa atgcaaaagt attgaatata ggaaaatcag
     1021 aaattataaa tatagatgaa ggtgataaag tagatacatc aacaaaggta gaaaataatt
     1081 caacatttaa tatatccaat gttgaattga tatataatga atatgatgag aataaaaaaa
     1141 ttatatcttc ggatagtaag gctttgttgg acatgacttt aatgcctggg aaagttgctt
     1201 acatagaatg tgggcataaa acttttgtaa agggtgtaga agtttatgca tatgaatatg
     1261 aggcagaggg taagattgtg tatgttaatt taaaagaaaa tactattaat ataagaaata
     1321 ataatattaa gctagaaaat agtagtcagt atgaggtgct atctacatca gaacttaaaa
     1381 aagtaaatga atcaaatgag ggaattacgt atcaagtaaa agtaaaaaat tcatcatcaa
     1441 aagatttagg aaatattatt cttaagacag cagaagttaa tgaaaatgga gaatatttaa
     1501 cagtaaatcg tgtgccatca tataagattt taaagccttc agaagagaca gatatagata
     1561 tattgtgttc aacaaaggca aaaagtgttg aaatagtggg atatacgtat gatgatataa
     1621 aagaaaaagc caatgtggat attgacttaa aatctcataa agttaagata gacaaataaa
     1681 ggtaggggat acttatgaag gttttaatag ttgaagataa taagatactt ttagaaagtg
     1741 tagtagaaga acttagcaag cattttgaaa ctgaaaagtg tgaagatgga gaagaagctt
     1801 tatacttagt aaatcaaaat atatatgatt tagtcatttt agacttaatg cttcctaata
     1861 taaatggatt tgatatccta aagaaaatga gagttaataa tatagataca cctgtactta
     1921 tattgacagc aaaagaaaca ttagatgaca aagtagaagc atttactata ggagctaatg
     1981 attatttgac aaaacctttt tatatggaag agttagtagc tagggtatac gctatattaa
     2041 ggactaatgg aaagataaaa gaaagaaacg gattagaatt taaatcttta tatttagata
     2101 cactagaaaa aagagtgtat attgaaaaag aagaaataaa acttcaaaac aaacagttta
     2161 acttgcttga gtattttgta ttaaataaag gttcaatttt attaaaagag cagatttatg
     2221 atagaatatg gggaattgat tcagatgcga ctattgaaat agtggaagta tatgtaagta
     2281 atctaagaaa aaaactaagt aaatatggat atgataaata tattaaaact aaacgtaagg
     2341 taggatatat attcgatgat aaataagaat gtatttacca gtactaaaaa tcatttaata
     2401 aaaatgtata ttattgtagt aggtagtttt ttaataatct tttctatatt tatatactca
     2461 tattttaggg gacttacata tagtggaatt gatagtgaga taaatgatga acttgaatat
     2521 atagtctctc aatttaaaag aacttcattt ttgaacccaa taagactgaa agatcctaaa
     2581 gatatggtgt atgtgtatga agatggaagg ataagttact atactcaaaa tgaatatttt
     2641 gatgagttac ttccagatag aagactagat aaaaaaaatt cgttttttaa atacacagaa
     2701 aatggatata cttttagaga attaaatgta gatgtaggta gataccaaat acagataatt
     2761 agaaatatag attctgagat gaattcttta agacagctta catctgttct tataattgga
     2821 atattaattt ctgtaataat tacttacttt gtggctgtat atctcactag aaaagcttta
     2881 attccaatag aaacagcatg gaaaaatcaa gctaagttta tacaagatgc ttctcatgaa
     2941 ttaagaacac caataacaat agtttcatca aaattagaaa gtatgttaaa atccccagaa
     3001 agtacagtaa atgatgaagt tgaaacaata gctactgcaa tgaaggaaac tagaagactt
     3061 aaaaaaatga taactgattt attgtctctt actaaagagg actctattgt taaagttaat
     3121 ttagaagaaa tagatttaga aaagttattg gaagaaatat cagaagacta tattgacatt
     3181 gctgagtttc aggaaaaaag attcgtgttt aattctaaac ttaagaataa agttataata
     3241 acagataaaa ataaattaag acagcttata ttaattttca ttgataatgc ctttaagtat
     3301 acaaaattgg gtgatgagat agcattagaa ttgaaagaag atatagagga tgaggtaact
     3361 ttacttataa gtgacactgg tataggcata aaaaaagaag agatacctct tatatttgat
     3421 agatttttta gaagtgaaaa tgtaagaaat aaagatttag aaggttctgg aataggactt
     3481 tcaatagctc gcatgattag tttaaactta tctattgata taaatgtaac ttctgatgta
     3541 gatattggaa ctacatttga attgtcaata cccaaaaagt taaaataata aaaatataaa
     3601 aaattgtttt tagaaatgaa gaatgggtat actaattagt ataaaatata aaaagacctt
     3661 ataaaagtaa atttatattt ataaatttca tagtataatt atatagggat aataaagttt
     3721 aataggagga tttaaaaatg gcaaaagtta taaatacatc tgaatttaga agtagtgttg
     3781 aaggtagtaa gggagtagtt gtagttgatt tctttgcaac ttggtgtgga ccttgtaaca
     3841 tgttgggacc tgtatttgca gaattaggtg aagagatgaa ggataaagct agatttgtaa
     3901 aagttgatat agatgagagc ttagaaatag ctcaacaatt caatgtatct acagtaccaa
     3961 ctatgattat atttaaagat ggaaaaccag tagaaacttt aattggattc atgccaaaga
     4021 acaaaattga aatgcaagta aaatcatatt tataaaaaat gggtgagtat tatgagatat
     4081 gatatagcta taatcggcag tggtccagct ggattgtctg ctgctataaa tgcaaaaata
     4141 agaaataaaa caattatcat gtttggtaat gataatttaa gtaataagct tgttaaagct
     4201 ccttcaatag ataattattt aggtttttat gatataagtg gtgatgaatt aaaagacaag
     4261 tttaaatcac atatagatag tatggatatt tcaattgaaa ataaaagaat aaataatatt
     4321 tatgctatgg gagaatactt tacaataatg tctggaaatg atatgtatga agctactact
     4381 gtaatccttg caactggggt agagtatact agaccaatca agggtgaaga agaatttcta
     4441 ggaagaggtg tgggctactg tgctacatgt gatgcaccac tttatagaaa taaaaaggta
     4501 gcagttatag gatataatga agaatctaaa gaagaagcaa attttttaag tgaactcact
     4561 tcaaagactt attttatacc tatgtacaaa aaagataatc ttatgagaag ttcagataac
     4621 ctagatgatt ctattgaagt tatacatgac agaccagtac agatagatgg agataaactt
     4681 gtaaacaaag tatcttttaa agaaaaccac attgaagttg atggagtatt cgttataaaa
     4741 gatagtacag ctccaagtgc attggttcct ggaatagaga ttgatggcat tcatattaaa
     4801 gttgacaata atatgaagac tagtatagat ggatgctttg ctgctggaga ttgtgttgga
     4861 aaaccatatt cttatataaa agcagctggt caagggcaaa tagcagcttt aaatgctgtt
     4921 tactatttag ataaattaaa gagggcttaa tatttatatt gattaacttt ttaaagatat
     4981 ttataaaaaa ctgtcttgaa ataattttat tatttcaaga cagtttttta tttttaagtt
     5041 attaataaaa gtattttaaa atctatatgt aattactttt attaataaaa ctcaaagtta
     5101 tattagtaaa aattggaatt atttataata tactccaaaa tttaaaaata atggttgaaa
     5161 aatctaatta agagtactat aatttaatta agtaattact taatcaaaac gaggtactta
     5221 tggaaaactt ggttaaaata tttaaagcat tgagtgatga aactagactt aacatactta
     5281 tattagtatc caagagaaat atatgtcaaa aggggatttc taaatatcta gggatttcag
     5341 attctgctgt ttcacaacat ataaaaatat taaaagatgt tgggataata acaggatata
     5401 aagaaggata ctatgtttta tatcatatta ataaagaatc atttaatata tgtgtagatt
     5461 ttataaattc tatgttaagt aattctagtg aaagctttat agatgtattt gatgtaaata
     5521 caatacatct aggatgcagt aaagaatgta aatctataaa aaaatgttgt aagagaaggg
     5581 agaaataata atgaaagtat gtataccagt agaggagaat aaaggattgg atagtaaacc
     5641 atatggacat tttggttctg ctccaatttt tgttgtgtgt gatttagaaa gtggagaagt
     5701 taaatcttta gataatggag atttagatca tgaacatgga aaatgtcaac cattaaaagc
     5761 attgtcaggg acagctgtag atgcagtagt tgttggagga ataggacaag gagctataat
     5821 taaattaaat ggaatgggaa taaaagttta cagagcagaa ggtgatacta taagtgctaa
     5881 tttagactta cttaaaaatg gaaaattagt agaatttcca tcagaccata cttgctcaca
     5941 tgacggatgt ggacatcatt aaaaaaaaac agctgatgaa atatatactt catcagctat
     6001 tttttctttt aataaatacg attttattct tttaaaacca aaatttagat ttttatattt
     6061 tttgtagtat ccttaataat atttcaagtt tacaagttta ctacttttag attttttttg
     6121 tatgtttttc tatatagtca atatcattta atatgtcatt gagttcttta ttaagtgttt
     6181 ttaaatcatc accatctaca ttgtagttta aaaatagttc ctctacatta tcaatgatag
     6241 aattgacttt tctagtaact tcaacaaatg tcttaaagtt actactattt ttagcattta
     6301 gaaatttttt taaatccttt gatataaatg atactatttc atattcatca ttaaatagac
     6361 ctctaccaat aggatatggt tcaattctct gtaaaaaata ttggtcttta gcattaatat
     6421 tataagtata agatatttcc caaccgtctc ctaaatcaaa cttgataaaa tataatgtac
     6481 tacttacttt tgatattctt gtggcaccaa gctgtcttaa aagaacttca atcgaatcac
     6541 cttgaacagt attaatattc atatatacac atccccttta gtattattat actatatttt
     6601 ttaaaatttt ataattaaat aaacagtaga tataataata gatataatca taagtggaaa
     6661 acctattttg aaatactcct taaaagagat aggatgaccg tgtttattac ctatacctgc
     6721 tagtacaaca ttagcagatg ctcctattag tgttccattt cctccaagac aagctccaag
     6781 agatgttgcc caccatagtg gcattacatc aataccttca gcttgcatag tcaaaataag
     6841 aggaattagt gttgcaacaa atggaatatt atctaagaaa gaagagacta tagctgacaa
     6901 ccataatatc aacaacattg taaatacaag atgtccttct gtaagtccaa tcaatgcttg
     6961 agccaatttg tttattatcc caacttctac taatccacca actactacaa ataatcccat
     7021 aaagaataaa attgtagacc attcaatacc tgccataatt tcatctacat cttgttttcc
     7081 tattaaaagc attatagatg ctcctgtcag tgctacaaca gaagaatcta tgtggattgt
     7141 actgtgaaac ataaaaccaa aaagtatgaa tgctattaat ataaggcttt ttataagtag
     7201 aggtttatct ttaacagatt ttttttcatc tagttttaat attgcatttt tagctctttc
     7261 attcacaaca agttcttttc cataaataaa tctaaagcat attatagtta tagcaagtat
     7321 tactataaca gcaggaccta aatttattac aaaatccata aaactaagat tggcagcact
     7381 accaatcata atatttggag ggtctcctat aagagtagct gttcctccta tatttgaagc
     7441 taaaatctgt gttattaaaa atggaactgg attaagtcct aatatttgtg taataactat
     7501 agtcattggt ccaataagta agacggttgt aacattatct aatacagctg ataaaatagc
     7561 tgttataata gcaaagcaaa tcattatctt ccatgggtca cccttagctt tctttgcagt
     7621 ccagatagct atatattcaa ataaccccga gtttttgaca acagctacaa atagcatcat
     7681 accaactaat acaccaatag tattgaaatc tatatggctt aatccttcat caagtgtaag
     7741 tatattgaat attatcatta atagtgcacc accaacagct gctacggatc tgtttacctt
     7801 ttctgtaata atagctgcca taactattaa aaatatacat atagctatta tttgttgaaa
     7861 ttccataata taagtacctc catttgtatt attttgtgcc taatgctagt actatatata
     7921 gtattacttt tttattgttt tttcaatgga tgtatgaaag tatattattt aacaaagttt
     7981 ttgaaaaatt gacagaattt aaagaaaatt tttgttattt aaataaattt taaaattatt
     8041 ccattgataa taatattaaa cgacaaacga aaaaatatac cctttaaact agaatttatt
     8101 gtatctaata acaattataa aatattaaat atatctatat tataaaaaca ataaaaaaag
     8161 gtttgatttg aatataaaac tgcaatatat taaaaaaatt attcatttta aaccttgtaa
     8221 tttgatttta taaatattat aattaaatta atgaattttt aacaaggaat tattcatttt
     8281 tttgatttta agatttattg agaaaacaaa actaaatagg ggggtaatta atataatgga
     8341 ttttttcaat ggtattttaa cgagtattaa caatgcagta gtatttgtaa acaattactt
     8401 atggtcgtat atcttaatta taatgttagt tgtaattggt atttacttta ctattaaaac
     8461 aaattttgta caattcagat attttataga aatgtttaga cttctgggtg atggtacagc
     8521 gaataaaaat gcaaaaaagg aaggaaaaat ctcttcattt caagcatttt gtataagtac
     8581 atcttcaaga gttggaactg gaaatatagc tggaatagca attgcagtgg tagcaggagg
     8641 acctggtgct gtattttgga tgtggcttat agcattaatt ggttctgcat caagctttgt
     8701 tgagagtaca ttagctcaga tttacaaagt gaaagatggt caagctttta gaggtggacc
     8761 agcgtattat atggaacaag gtctaaataa aaaatggatg ggtattttat tctcaatact
     8821 tataactata tgttatggtt ttgtatttaa tgcagttcaa gcaaatactg tttcacttgc
     8881 atttaataat gcatttggaa taagtaaaat ggctatggga ataggtcttg gaattttaac
     8941 tgcaatagtt atttttggtg gagtgcatag agttgcaaaa gtttctgaaa taatagttcc
     9001 tgtatttgca ggattatata tattagttgc tttggctata gttgtaatga atataacaga
     9061 gataccaagt gttatagcat tgatagttga aagtgcattt gattttaaag gaatggcgat
     9121 tggtacattt atgggagttg taatgactgg agtgaagaga ggtctatttt ctaatgaagc
     9181 aggtatgggt agtgctccta atgctgctgc aacagcacat gtaactcatc ctgtaaaaca
     9241 aggtttaatt caatctttag gtgtttttac agatactata ataatatgta gttgtacagc
     9301 attcatggta ttattatatt caggttatag tagtagtgga gcaactggaa ttgaattagc
     9361 tcaagaagct ttaactcatc atataggacc agttggaaat atatttatag cagtttgtat
     9421 attcttgttt gcatttagtt ctatagttgg taattactat tatggtgaat caaatatgga
     9481 gtttatgagt ggaagtaaga ctaaacttaa tgtatttaga gtttttgttg taggtatggt
     9541 tttatttggt tcacttactc aagtggatgt agtttggaat cttgcagact tatttatggc
     9601 attgatggcg ataataaact taatagcaat agcactttta ggaaaatatg catttattgc
     9661 tttaaaagat tatacaagtc aaaaaaaatc aggaataaaa gaccctgtgt ttattgcaga
     9721 tgaaatagaa ggcctagaaa aggtatcaga gtggcatagg gaattatctg aggaacattt
     9781 aggataaaaa atagtataaa attataaaac cattttgaaa gactagagta tgtatatact
     9841 ttatatgaat ataatattga attaatttat attactggaa tttttaattc cagtaatatt
     9901 tttttaatta caaggatatt atgatgactt aaagactgtg gaaaactctt ttttagtttt
     9961 cattattaat attaattgac aaatttagtt tttatttaaa tttcttaagt gcatataaat
    10021 cgtatttttt gataatccca ttaagtctgc aactttaaca acagaatcct taattttaaa
    10081 aataccactt tcatttaaaa tagaaacaat agatttattt ttatcattgc tagatatatt
    10141 agaatcattt tcaacaatta cctttgcatc ttggtatgat tttaaaatta cttcatcagt
    10201 atcttctacg atattttcta aaacaggagc actttgcata gcattatgtt ccggaatata
    10261 tgaaccaatt attcttgtta gtggtgtatt aagataaaaa tgtatgcaga gtagaccgat
    10321 tattttatta tcaactcccc ttatcactat atctgttgat tttaaaggtt cacctagttt
    10381 gtttctatta aaataactga taaaatctcc atcagcatca ttttcgagtt tattaatgag
    10441 tgccaaatca aaatcagtta taatatctcc ttcttttaca gtattatatt taccattagc
    10501 tattttattt attgaatagt gtactccatt aaatttatat aaaataaact cgtatccttc
    10561 tcctaaatac gcagataatc catccactag attgttataa gaatcgaaaa tatagttatc
    10621 agtcatgtta aaagtcatat gttacctcct aagaaatatc ttaatatcat attaacatat
    10681 aaaatgaatt taataaaata tatttcagtg aaataataaa tataatgaaa aaaaaactat
    10741 tgactgaaaa atatttcagt gatattatta aaatacagta aggcaaacgt attattgaca
    10801 gaatatataa aatgttaaaa atagtatcta ggaattaaac aaattaaagg actagtgatt
    10861 tgaatttttt tagtataata tgtgacttcc aattaatatt tattaaacac caataaaatt
    10921 gtaaatatgc ttaatgatat actttagcga tgaagtgaaa atattttatt tttatgatta
    10981 acctatagga attaatttag catactgttt agctagtaaa tctaaataaa ttttagcttt
    11041 agctgatatc atgttgaata aagtttctat tggattttgt ataacaaagg agtggggaga
    11101 tggcacaaga taaaattgtg gttgaaattt tagaaaacat tggtggtaag gaaaatatta
    11161 aggaagtagc acattgtatg actagactga gaataaaggt atgtgatagt tctcaggtaa
    11221 atcaggaaaa actaaagaaa ataaaaggtg tactaggtct tgtattaaaa ggtgagcaat
    11281 atcaaattgt tataggtcct aatgtatcac aagtgtataa tactcttaat gaagttatag
    11341 gaattatacc tgagaaggat agtaaaaaaa caatatctga aggtaaacaa aatagtattg
    11401 caaaaatatt agatactatt gcaggtattt ttaatcctat agttccagca ttagcgggtg
    11461 caggtctaat aaaagctata ttagcattac tagtagcatc aaagttttta tctgcagata
    11521 gtgaaaccta tataatatta aatacaatat ctgatggtgt attttcattt ttaccattta
    11581 tgttagctta ctcagcagct aaaatattta aaatgaatac ttatgttgct gtagcaatag
    11641 caggagcaat gctacatcct aatttcatta atttaattca acaaggctta actaaaatat
    11701 cttttatcgg tattccgatg tcactaatcc agtataacgg aagtgtattt ccgatagtac
    11761 taactatttg gtttgcatcg tatatagaaa aaggagtaga taaaataaca ccaaaggcac
    11821 ttaagataat attagtacct gcattgacta ttttatttgc agctcctata gcattaatta
    11881 ttattggacc aatagctcaa atgctaggaa actatatagc cggtggagca agtatcctat
    11941 ttgaaagagg aggaatattt gcagggttaa tttacggagg aatttattca ttcatggtag
    12001 taacaggatt acatcatggt atggttcctg tattagtaga tggaataaca agatatggat
    12061 ttaactatat aagtcctgtg tcaggtagtt caaatatggc tcaagcagga tctgcatttg
    12121 gagtatggtt aaaagcaaaa gataaagatt tgaaggctgt agcagctaca gctaccatat
    12181 cagcattaac tggagttacc gaacctgcaa tatatggtgt taatttaaga ttaaagaaac
    12241 cgtttttatg cgctgctata ggtggtgcag caggaggtgg atttgctgca tatttccaat
    12301 gtaaagcatt tgctatggga ggtccatcgt ttttaactct tcctatgttt ataggagaag
    12361 gtggacatgt aggattggta gcaatagcat ttacaatagc atttatagta tctgcaatag
    12421 ctacctatat agtaggattt gaagaagagg taacagcaga ttcacacgct aacttataat
    12481 aaagagaaat tatttaacat atcaaaaagg agattaatta ctatgaaaat ttatttaact
    12541 aaagattatg aagaaatgag ccaagtggca gcacaaatgg tgttaaagta tatgtacagc
    12601 aatgataata aaagaataaa cctttctata acagcaggtc aaacaccaaa aagaacatac
    12661 gaaatattaa gtgaatttgt taagaataaa ccttattttg aaaatgtaca ttattataac
    12721 tttgatgaaa taccaataaa aggcgaaaaa gttggagtta ctataagaaa tttaactaat
    12781 atgtacttta gagaagctca aataccagaa gaaagaatac atgtgttaga tgaaaataat
    12841 tatcaaggct ttgatagaaa gatagaagcc gatggtggaa tagacatggt attaatggga
    12901 ttaggttggg atggacactt ctgtggaaat cttagtggta cagtcaactc ttttgatgaa
    12961 gagacaagag ctgtttcaac ttatataaca gatgatatgg ctaaactatt aacaaaatct
    13021 gtaggtggaa atccagaaaa attatttgat tactatgtga catatggacc taaaacaata
    13081 atgcattgta aaaatataat aatgatagta aacggaaaac acaaagctca aatattaaag
    13141 caaacactat ttggaccatt agatttatta tgtccatcat ctttattgag attacatcct
    13201 aacttcacat taatagtaga tgaagaagca gcatctgaaa taaaagattt aatataaata
    13261 agtaaggaat taaaattaaa aatgaattta ggtgcaaagt aatattttat tttgcacctt
    13321 tgctttaata aaaaggagaa taaaaatgta taatcatatg aaaaaagtat tccaaggatt
    13381 agagggtggg ttattttcaa gtgtagagaa agctgatgta ggcgattctt atcaaaaatt
    13441 aggtgagcag ggtgtaagtt taatgggatg ggcagatcca tttttcccag attactgtat
    13501 acctagacat atattagatg cagctacatt agcgatatca agtaatatgt caactcatta
    13561 tacagcgcct gtaggtaatg acttattaaa aattgagata gcagaaaaat taaaagaagt
    13621 aaataattta gatgttgatt ttaaaagaaa tatattaata actcctgggt cagactcagg
    13681 gctttattac tcaatgttac cgtttataaa tgaaggggat gaggttctta taccaacacc
    13741 atgctatcca aataatatac aaaatactaa attaatggga ggagttccta tatttgtaga
    13801 gttaaaggaa tcagatggat atcaaataga tataacagaa tttgagaaga gattaactaa
    13861 taagacgaag ttagtagttc ttactcatcc taataatcca acaactacaa tatttaataa
    13921 agaatcttta gaaaaattag ctaaatttat tatagacaat gatttaatat taatatgtga
    13981 tcaagcattt gaagatttta cttttgaaaa tgaattaata acaccagctt cattagaagg
    14041 tatgtttgaa agaacagtaa caatattttc aacatcaaaa ggtatgggat taagtggatt
    14101 tagagttgca tatatggtat gcagcgataa attaatggac aaatactatg gagcagcagt
    14161 gacagtatta ggtgctacta atacagtagc tcaaatgtca acgattgaag catttaaaga
    14221 tagaagcttt atggaggatt ttgaaagagt ttttgataaa agaaggcaag aagcatataa
    14281 gataatcaat gaaataccta atgtatctat gcagatgcct gagtccggat ttttaggttg
    14341 gataaatgta tcaaagcttg gaacatcatc agaaatacaa aagtatctag ttgacgaggc
    14401 taaagtttgt attaatgatg gaattaatta tggtccagga ggagaagggc atttgagaat
    14461 aattttagga agctataaag acgatagtaa ggtaattggt gcattaaata gaataaaaga
    14521 tgctttaatt aaaaagcaac aataaataaa aaataattgg agtggagtat gttatcaata
    14581 tttaagagta aaaaaaatga caacaagctt aaggcatttg catcagggaa agtaattcct
    14641 atagaagatg ttgatgataa tgttttttca tctaaaatgt taggtgatgg aatcgctatt
    14701 tatccaacta atgaatatgt tgtagcccct tgtgatgcag aagtaaatat aataatgaaa
    14761 gatactaaac atgcagttgg aattaaatta tttaatggta tggaaatctt atttcatgtt
    14821 ggaatagata ctgtatctat gaatggagaa ggttttgaga tatttgtttc accaggtgac
    14881 aagataaaaa gaggtgataa attaatatct tttaatagta aattaataaa agaagaaggg
    14941 ttacaagatg tagttataat ggtagttact aattcagaac agtataaaaa tataaaatat
    15001 catattggac tggaggcagt aatcgatgat actattattg cagaaattta aaaacagtta
    15061 tttaagtaaa tacttagata atttagatat agttaaaatt aaatccttaa aatcaaatta
    15121 tttaattttt gtgtaatttt aactataaag ccgattaata atataaagta tactaaataa
    15181 tatggttatt atgattgtta ctgacaaagc ctaagtagat ataacaattt gctctaattg
    15241 agtattgcta tatctacttg tttttatatt taaaataaaa gagtctgcat tgaaaagata
    15301 attctgatga gagtaaatag ggtatttaat aatagtttaa ttctgactta tcaagatgaa
    15361 cctattcata tacctaaaac atctttaaca ccttctaaat tcatacaaat agctgtatag
    15421 gctgccttat tgattatttt tccgtcaatt tttactttgt agtgaacagc atatataaat
    15481 attatcgaat agattgtatc aagagctcta ttttgctatt cattagctaa agatagtact
    15541 ctgtcagtta tttttgaaac taatgcatga gatatatcaa ttccgtaaat attatttaaa
    15601 tgtatgatat atatcgcgag tcgacattcc ttttgagtac atgccaatta cttggttctc
    15661 tattccagaa atttcatttt cgtaattttg aataacccta ggctggaaat catccctagg
    15721 gatatttaat tttacctctc caaaatctga tttaacattc ttagctctat atccatttct
    15781 tgaatttgat gttgctttac tttggttatc atatctctcg tacattaaat gatcttcaat
    15841 ctctgactct aacattttgt gaattgtgct tgcgaataaa tcttttaaca tctcttggat
    15901 gtctttagta ttttttacat catactcttg aactaagtgt tttaatattt aatttttgtt
    15961 gtacataaaa aatgtgtacc tcctattttg gtaattagat ttaatatctt gacttttagg
    16021 agaatacaca ttttatttta cagaccctct tttctagaaa agtttttatg aaaattgtga
    16081 tataaattat taataaaaaa taattttata tttattttat tatgtaattt agttttatta
    16141 aaaatttttt attattggat atagattatc tattataaat tttatcttta agtttattct
    16201 cctaaaatac tatataatta aatcctgtaa attatcttca aagctttcta ggtaatcaca
    16261 aaataatcta aattcttcta tggttttaat ttcttgtaaa tttctcaaat ctatcattat
    16321 attactatat ttatgaaaca taaaattcat atcatacatt tcacatttaa caaattcatt
    16381 ttgataacta tatttataag gttcgtattt tgaaaaactc gactcaaaat attttatttt
    16441 agcataacat aatttgtgta tgttgaacaa aagtgcaaga acagactcct taactgcaaa
    16501 cttatgtgta aagtatttta tcgtgtggta tttaccatgt ttaaactccc aaattaaatc
    16561 ctcatttaaa gtaagagaat atttttttat aaggttacgc gtatctagtg ttaaattttg
    16621 tattgaatgt attttcataa tttattctcc tcataaa
//
LOCUS       DACULQ010000080        16619 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.080, whole genome shotgun sequence.
ACCESSION   DACULQ010000080 DACULQ010000000
VERSION     DACULQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.080"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            77..1477
                     /locus_tag="JA154_10390"
     CDS             77..1477
                     /locus_tag="JA154_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAT7996271.1"
                     /translation="MNQEIFFKIMNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMN
                     RHDILTNSFFDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPID
                     IKGEFVGALEISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAI
                     AKSKKACKSDASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGIL
                     FGTSKGGFTGAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTK
                     DIPIDVRIIATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFI
                     SKYNKKLNKKVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINL
                     NDYYTLKENKESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMN
                     RQNLQYKIKKYNIKIL"
     gene            1733..2800
                     /locus_tag="JA154_10395"
     CDS             1733..2800
                     /locus_tag="JA154_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HAT7996272.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKINFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2921..3229
                     /locus_tag="JA154_10400"
     CDS             2921..3229
                     /locus_tag="JA154_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HAT7996273.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3240..4652
                     /locus_tag="JA154_10405"
     CDS             3240..4652
                     /locus_tag="JA154_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HAT7996274.1"
                     /translation="MKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYEKMMSET
                     GYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNASGSFKA
                     RRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNGVGQPEI
                     IEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVETLGYELA
                     IQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLHMASDNQ
                     FNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTTELLAQL
                     EGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARENGIDIK
                     FGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKLSNRDIE
                     FLMEEVKSSRDFVVDVLNNL"
     gene            4730..5104
                     /locus_tag="JA154_10410"
     CDS             4730..5104
                     /locus_tag="JA154_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase"
                     /protein_id="HAT7996275.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5098..7302
                     /locus_tag="JA154_10415"
     CDS             5098..7302
                     /locus_tag="JA154_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HAT7996276.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIIMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7302..8711
                     /locus_tag="JA154_10420"
     CDS             7302..8711
                     /locus_tag="JA154_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HAT7996277.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8696..9760
                     /locus_tag="JA154_10425"
     CDS             8696..9760
                     /locus_tag="JA154_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HAT7996278.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9790..11211
                     /locus_tag="JA154_10430"
     CDS             9790..11211
                     /locus_tag="JA154_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAT7996279.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11640..12104
                     /locus_tag="JA154_10435"
     CDS             11640..12104
                     /locus_tag="JA154_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7996280.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLVKLDVEVGFPVATYIDGKNEILQKIVPAQKIITAIDL
                     GLYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12338..12700
                     /locus_tag="JA154_10440"
     CDS             12338..12700
                     /locus_tag="JA154_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAT7996281.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13284..14120
                     /locus_tag="JA154_10445"
     CDS             13284..14120
                     /locus_tag="JA154_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7996282.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            14204..16318
                     /locus_tag="JA154_10450"
     CDS             14204..16318
                     /locus_tag="JA154_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAT7996283.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTCELTQLIDM"
BASE COUNT         6577 a         1840 c         3105 g         5097 t
ORIGIN      
        1 atgataagat aaatacaata gcaacatttt attaattttt tgggttttta tatatttagg
       61 tactggggtg atatagttga accaagagat attttttaaa ataatgaatg aaatattgga
      121 aaatattgat gaaggggtac attttgttaa taaagacggg aaaactataa tctacaataa
      181 ttctgtacaa aaaatggaaa aaatgaacag acacgatatt ttaaccaatt cattttttga
      241 tatagtcaac aaaatgaaaa taaacaggag tacactatta gaagttttgg aaaaaaggga
      301 agttatcaaa gacaatattc aaaaatatct ggataaaaat ggaaaagaga taacagcaat
      361 taatactaca ataccaattg atattaaagg cgagtttgta ggggcacttg aaatatcaaa
      421 gaatatgaca acgatagaaa atttactaaa tagaaattat aaaaaaaatt tagtcaataa
      481 agttagtatt agacaaaaaa agggttatga atttgatgat attttagggg aaagtaattt
      541 gattaaagaa gcaatagcta aatctaagaa agcttgcaag tctgatgctt cagtatttat
      601 atacggtgaa actggaacgg gtaaagagtt atttagccag tccattcact atggaagtaa
      661 taggagaaac tatccattta tagcacaaaa ttgtgcagct ttgccggaat cactattcga
      721 aggtattctt tttggaacta gcaagggagg atttacagga gcaatagata ggccaggctt
      781 atttgaacaa gctaacaaag ggacattgtt attggatgag ataaattcca tgcctattat
      841 gttacaggct aaactattaa gggttttaca agaaggttat gtaagaaggg ttggaggaac
      901 taaggatata cctatagatg taagaattat tgcaacgaca aatgaaagtc caaaggtaat
      961 tttaaacgag cataaaatgc gaaaagattt atattatagg cttaacataa ttcatataaa
     1021 aattcctcct cttagagata gaaaagagga catacctcta ttgtgcaaaa aatttatttc
     1081 aaagtataat aaaaagttaa ataaaaaagt ggtaggaatt tcagatgaag ctattaaaat
     1141 tttaaaaaat tacgattggc aaggtaacat aagagagcta gaaaacataa tttattgtac
     1201 tatgagtatg atggaagatg aggtacaaat agaaacaagt atgattaatc taaacgacta
     1261 ttacactctt aaagagaata aagagagtaa caattattct ttaaacgatt tggaggacaa
     1321 aacacttagt aagattatca gtgagataga agaaaagtat atatatgaat cattagaaaa
     1381 ggcatcttac aatataacta aagctgcatc aattttaggt atgaataggc aaaatttaca
     1441 gtataaaata aaaaaatata atatcaaaat attataaata agcaagataa aaaaacaaaa
     1501 tcaaagtaaa ataaaaaagc aaaatagagg agcaaaatta ttataaaaga gcaaaatatt
     1561 ttgctctttt ttttatttct cataagaaat attatttttt atttatgaaa atacataata
     1621 aaataattat agtttcaacg ttccaataaa tatttacttt aattttaaag ttggcacgat
     1681 ttatgcttta actaattatt gagaatatat aaatttttag gagggtgtat atatgagaaa
     1741 agtaagagta ggaatatggg gatttggagc tatgggaata ggtatggcta atatgattct
     1801 aaagaaagaa ggtatagaaa tagtatctgt ttgtagtaga agtacttcag ggaaaagtat
     1861 gtatgatgtt ttaggaattg aaagaggaga aagaccagaa gttattatca ataaaaatta
     1921 tgaagaagtt tttagagaaa aaagtgtaga tgttgttttg ttagcaactg attcatttac
     1981 taaaaaggct tttgataaaa taaatttctt attaaataga aaaataaatg ttatatcaac
     2041 agcagaacaa atggcatatc cacaagctga tgatgcagat ttagctaaaa aaatggatga
     2101 agttgcaaag gaaaatggag taagtatact tggaacagga ataaatccag gatttgtatt
     2161 agatttacta gtattggcac tttcaggtac ttgtgaagaa gttactagta taaaagctaa
     2221 gagagtaaat gatttatctc catttggaaa gtctgttatg gtggaacaag gtgttggagt
     2281 aacaagggaa gaatttataa aaggtgtaga agataagact atagcaggtc atgtaggttt
     2341 tgtagaatca ataaatatga tagctgatgg attaggttgg aaattagata agatagaaca
     2401 aacaaaagag ccaataatga ctacagtaga tagaaaatct aagtacggag aggcattggc
     2461 tggaaatgtt gctggttgta gacaatgtgg atatggatat gtaaatggag aagttttaat
     2521 tgaaatggag catccacaac agataatacc agaagcagaa ggtattaaaa caggagacta
     2581 tgtatcaata aaaggtatac caaacattga tttacaaata aatcctgaaa tacctggagg
     2641 agtaggtact tatgcaatga tagtaaactc tattccatta ataataaatg ctagaccagg
     2701 tcttaaaact atgttggata taccagtacc tagagcaata atgggagata taagaaatca
     2761 gatagaagtt gaattggaag aagaatcaaa ggctaattag taagatagca actagctaat
     2821 tagtaagata acagttagct aattggtaaa ataatgttta atttaaaata acagtaaatt
     2881 aattagtaag gcaatagtca attaataagg ggatgaaaaa atggatgcta aaattaatga
     2941 ttgggttatt atacacaata tagtattgac tccagaagaa agagctccac aagttccaga
     3001 ggatactaag aaagtatcct tagaaatgtg ggttaaaggt tttatacaaa gtgatgcttc
     3061 tataggcgac ttagtagaag ttaaaacaat tacaggcaga cttgtaaaag gtaatttact
     3121 taaaataaat ccatattata ctcatgatta tggtaaatgt ataccagagt tactccaaat
     3181 aggaatccaa gctaaagaaa tattatttgg aggtgtatat aatgaataat tcaacatcaa
     3241 tgaaagatat gagttatcaa gctgttatgg gaagaaataa cgaaataatg aaaaatgcaa
     3301 tcggattgga ttattcttct tttgaacaag agggaatagg gtttgattat gagaaaatga
     3361 tgagtgaaac aggatataca ttacaagata ttgaagcaat tcaatcacag tatgctgttg
     3421 gaaatactcc tttaatagag cttaaaaatt tgactaaatt agctagaaaa tgtgctaaag
     3481 aaggtaaagg agctagaata tttgtaaaag atgaagcaat gaatgcatct ggtagtttta
     3541 aagcaagaag agcagctact gcagtatatc atgccaaaca aatgggatat aaaggtgtaa
     3601 tagcagctac cagtggtaac tatggtgcag ctgtagcatc acaagcggca atgcagggat
     3661 taaaatgtat aatagttcaa gaatgttatg attctaatgg tgtaggtcaa cctgaaatca
     3721 tagaaaaagc gcgaaaatgt gaggcatatg gagcagaagt tgttcagtta actgttggac
     3781 cagagttatt ttatacgttc ttagttttat tagaagaaac aggctatttt aacgcatcat
     3841 tatattcacc atttggaatt gcaggtgtag aaactttagg ttatgagtta gctatccaat
     3901 ttagagaaaa gtataaaaaa gaccctgata tagttgtttg tacaaatgca ggtggtggta
     3961 atcttactgg aactgcaaga ggattgataa aagctggttc tattaatact aaagtagtag
     4021 gagcaagtgt agatttaaaa ggattacaca tggctagtga taatcaattt aataaaaaat
     4081 catttacaac aggacataca ggttttggga taccatactg tacatggcct gataggtcag
     4141 atgtacctag gtctgcagct agaccactta gatatatgga tagatatgta ttagtaaaac
     4201 aaggagaagt tttttataca actgaattat tggcacagct agaaggtata gaaagaggtc
     4261 cagcaggaaa tacatcttta gcagcagcat tctctttagc tcaagagcta gatgaggata
     4321 aagctatagt ggtacaagaa acagaatata cgggtgcagg taaacatata tgtccacaac
     4381 ttacctttgc aagagaaaat ggaatagata taaaatttgg aaatccaaga gaagaaattg
     4441 caggagtaaa cttaatatta ccagaaagac cagaattatt gaaatgtgtt gatattgata
     4501 tgaataaaat aagaaagtca tttataaaaa attgtatatc taataatcac attgataata
     4561 ttgataagct aagtaatcga gatatagaat ttttaatgga agaagtaaaa agttcaagag
     4621 attttgttgt tgatgtatta aataatttat agtaattatt gttttttaat ttaatataaa
     4681 ataaaaattc agaaaattcg attaaatcaa tacgaatgga ggaatgttca tgaaaaaaag
     4741 agaggatgat tttgaagtta gaagaaaaca tcttcaagaa ctgagtgatg atgagttaaa
     4801 agaaaggttt tggagtttag ctacccagat agtagaacca atgatagaac ttggtaaaaa
     4861 gaatacaaca ccatcaatag aaaggtctgt cttgttgaga atgggatttt cttctcttga
     4921 agtaaaacct attttagaag gggttatgga aagaggtctt ataggtaaag gagcagggca
     4981 tgtagtttat aaattggcta aatctaaaaa tataacggtt agagaagcag gtttgttatt
     5041 agttaagggt gaatattggg atgaagttac atgtttattt agagaagggg tagaatcatg
     5101 ttaaaggaaa ataaaaaatt ggatatagac tatatattaa aagatttgga taaatataaa
     5161 ccaaaacgaa gaggttgggt ttggagagaa cacttggaaa atcttgaaat gggacctttt
     5221 aaatataaag actgtacaaa acctttaaag aagagtgtag ggctaccatc atctaagtat
     5281 ttcaataata tagaccctca accaagtcca gttattacta ctgaaatagc ttctggtaga
     5341 tttgaggatg atataagaag aatgagaatg gcagctcatc atggagctga ccatctaatg
     5401 gttatcagaa ctgctggaca atcacatttt gatgggctta tagaaggaac cccacaaggt
     5461 ataggtggag ttccaataac aagaaaacaa gtaagagctc aaaggaaggc tcttgatttt
     5521 atagaagaag aagttggtag acctattaat tatcattcat atgtaagtgg tgttgctggt
     5581 cctgaagtag cagtaatgtt tgctgaggaa ggagttaatg gagcacatca agacccacaa
     5641 tataatgttc tgtataggaa tataaacatg gtaaggtctt ttgtagatgc ttgtgaggca
     5701 aagaaaataa tggcttttgc caatatagct caaattgatg gagcacataa tgctaatgct
     5761 actgctagag aagcatggaa ggtaatgcct gaacttatgg ttcaacacgc tttaaactct
     5821 atattctcag aaaaaatagg tatagataaa agtaatatat gcttatcaac agttccacca
     5881 acagcacctc cagcaccatg tttaaaaata gacttacctt acgctgttgc acttagagag
     5941 ttatttagtg attataagat gagagctcaa atgaatacta aatatatgga atcatcaact
     6001 agagaagcaa cagttactca tgtattaaac ttattaacat cagtattaac tagagctgac
     6061 atacaatcaa ctataacacc tgatgaaggt agaaatgtac cttggcatat atacaatata
     6121 gaagcatgtg atacagcaaa gcaagcttta gttggtatgg atgggcttat ggatatgata
     6181 gaacttaaag atgtcggtga attaagagac aaagcaagag aacttaaaga aagagctgta
     6241 ttatatatgg aagaaatatc tgaagttggt ggatattttg aatctgtgga acaaggattt
     6301 tttgttgatt ctggaaatta tccagaaaga aatggtgatg gaatatctag aaaaataaaa
     6361 ggtggagttg gtgaaggtac tgtttatgaa agagaagaag attatttagc acctgtaact
     6421 gctcactttg gatacaataa tgtagctcaa tatgatgaaa atgctataga taatccatca
     6481 atgttaatag atggatgtac atttgaaaat ccagacaaaa taatatatat tgatgagctt
     6541 gacgattttg ataatgtaga aaatagacta aaagaatcat atgagtacag aaatggaact
     6601 aaaattaagc ctgaaatgga atggtgtgca gatggaatta taatgataac aatgatgctt
     6661 cctactgata aaagaacagc agagtttgca gctttggaat ttgtcaaaaa aatgaactta
     6721 caagaaattg aggtcataag tagagaagtt atgcatgaat ctgaagggac tagaatagaa
     6781 gtgaaaggaa gagttccgtt tgatatagat ttaaacaatt tagtaatacc agaagaacct
     6841 aaggtactaa ctgatgaaga aataagagca gatatagaag aaaaacctat gaaaatagtt
     6901 tcagctacgg ttggagaaga tgaacattca gttggtttaa gagaaataat agatataaaa
     6961 catggaggta ttgaaaaata tggcatagaa tgccactatc tagggacttc tgtacctgta
     7021 gaaaaattgg tagatgcagc gattgaactg aatgcagatg caatacttgc atctacaata
     7081 ataagtcatg atgatattca ctataaaaac atgaaaaaat tacacgatta ttgtgtagaa
     7141 aaaggtataa gagataaagt aatgattgcg tgtggaggaa cacaagtaac acctgaaata
     7201 gctgttgaac aaggcataga tgctggattt ggaagaaaca gtaaaggcat acatgtagcc
     7261 actttcttag ttgaaaaaag aagagaaatg agtaataaat aatggaaaat agcaaggttt
     7321 ctttaaaaat agatgtgttg gtagctgaaa ttggaagtac tactacagtg gtaaacgctt
     7381 tccacgatat taatactaac aatcctatat ttttgggtca aggtcaagct ccaacaacag
     7441 tgtttgaagg tggcgatgta agaaatggct tatcaggtgc tattaaagat ttagctaata
     7501 aattatctgt agatgatata gagtataatg atatgtttgc aacttctagt gcagctggtg
     7561 gattaaaaat gactgttcat ggattagtct atgatatgac agtaaaagca gctaaagaag
     7621 cagctttagg ggctggagct gttattcgtc aaataacatc aggtagaatt aaaagaactg
     7681 atttaaataa aataaaagaa ataaacccaa acataatttt aatagctggt ggagtcgatt
     7741 atggagaaag agatacagct atttataatg cagaaatgat agcttctatg aatctaggca
     7801 taccagttat atatgctggt aatgttgaaa atcaagagga aataaggtta atatttgaag
     7861 atactaatta taagctttat ataactgaaa atgtataccc taaaatagat ttattgaata
     7921 tagaacctac aagaagaatc attcaaagtg tatttgaaga acacataact acagcaccag
     7981 gaatgaaata cattaaagaa atggttaatg aaaatattac tccaacccca ggagctgtaa
     8041 tggaagcttc taaactttta tataaaaata ttggagattt attaactctg gatgtaggtg
     8101 gagcaactac tgatgtacat tctgttacag atggaagtga ctatattaat aaaattttgg
     8161 ttaatcctga acctactgca aagagaagtg ttgaggggga tttaggtgta tatgtaaata
     8221 tgaagaatat agtagaagtt ataggtaaag aaaacttaca aagtgaacta tctatagata
     8281 ttgatgcagt aatagagaat tatcctccaa taccaaaatc aaaagaagaa atattatttg
     8341 ttgaaagatt gacaaaagaa gctgttgtta aagctatgct aagacattca ggaaaaataa
     8401 gaaatatcta tggaacaact ggaaaagtca aaattgcaga agggaaagat ttaacagaag
     8461 taagatatat agttggtaca gggggagcac ttactaggct ccctagtaga attgaaatat
     8521 tagataatat gctcaaatac aataaaaata atgaattact atttccaaag gaaaaaacaa
     8581 agatattaat agataatgac tatattatgg catctatggg tgttttatct aaaaaatatg
     8641 aagaagcatc tcttaagctc ctgttaaaaa gtttgaattt tgaggaggaa agattatgta
     8701 tcctaggtta gaaatagata tagaaaagtt aaagcataat gcaaaattaa tttcgcagat
     8761 gtgtcatgaa agaaatatta agatatcttt tgttactaaa tcattttgtg cacaaaaaga
     8821 aatagtagaa gaaatatgta gtgaaggaat agaccatata gcagattcaa gaatacaaaa
     8881 cttaaaaaat ttacaagata taaatctacc taagatattg attagaatcc caatgttaag
     8941 tgaattagaa gaagttatca attattgcga tattagtttt aactcagaat taggaacaat
     9001 aaagaaatta aatgaattat gtgaaagtaa aaatattatt cataagatag ttcttatgtt
     9061 tgacttaggc gatttacgag aaggatactt ttatgaagaa gactttttta ataatgtaag
     9121 agagatagta agattaaaaa atattgaaat aattggtata gctacaaact taacctgtta
     9181 tggagctatt ataccatcta gggaaaatat aggcagactg gtaagtatag caaaaagaat
     9241 ggaagaaaaa tttggaataa atctagaaat agtatctgga ggtaattcaa gttctattca
     9301 tctgttgata aataataata tgccagaagg tattactaat ttgagaatag gcgagtccat
     9361 tctacttggt agagaaacgg catacggtga aaatataaat ggaacatatc aagatgcatt
     9421 taaattaata tgccaagtag ttgaatgtaa agaaaaacca tcagttccta ttggagaaat
     9481 aggagttgat gcctttagaa ataaacctgt ttatgaagat aagggaatct taaaaagggc
     9541 aatcatagct ataggtaaac aagatataaa tattgactct ttaattccaa tagacactga
     9601 tataaaaata ttaggggcaa gttcagacca catgatacta gatgtaagta acacaaaata
     9661 tgattataaa ttaggtgaca atttagaatt tttattaact tatgggggta tcatgtcatc
     9721 tagtacgagc aaatatgttt caaaaaaaat tatagtgtag tgtttaaata attaggagga
     9781 aactatttta tgaaagagcg agaatgtaga aaagcaagtc tgtttgatgc aatgatacca
     9841 attacttgtt tgatattatt tttaagtcta ggagtattag tgtatggttc atcacctcat
     9901 gtaccactaa taggagcagc agcagtagca ggacttgtgg ctgtatatag acttggattt
     9961 aaatggaaag agttagaatt aagtatgttt aatagtataa agatggcaat gcaagcaatc
    10021 ttaatcataa ttataatagg cgtacttata ggaacttgga tagtaagtgg agtagttcca
    10081 tgtatgattt actggggact aaaaatttta tcaccaaata tttttttagt agcatcaaca
    10141 ttagtctgtg caatagtatc tcttgcgaca ggttcatctt ggagcacaat gggtacagta
    10201 ggagtagctc ttttaggaat tggtcaaagt ttaggtatgc caattggatt aatcgtaggt
    10261 tctataatat ctggagctta ctttggagat aaactatcac ctttatctga aacgactaac
    10321 ttagctccag caatggcagg aacagatttg tttacacata taaaatatat gctatattcg
    10381 actataccat cattattaat ttgtttagta atttatggtg taattggaat gaagtattca
    10441 ggtcaagcac tagatataaa acaaatagaa ttaattaggt ctacattaga ttctacattt
    10501 aatactttat caccagtatt attattagct ccagcaatag taattggatt ggttgtattt
    10561 aaagttcctg ctataccagg gcttatagta ggtgctgtat tgggagtatt gtttgcagta
    10621 gtttttcaag gagaatctat gaatacgata ttagatgcag cacaaaatgg atatgtttcg
    10681 tcaacacaaa tacaggaggt agatgcttta ttatctaaag gtgggattat gaatatgatg
    10741 tccactgtat ccttaacaat atgcgcttta tctcttggag gtattttaga aaaaacaggt
    10801 atgcttgaag tagtagcatc ttctttacta agattagcaa agggtgtatt tggaacagta
    10861 ttatgtacta tggtaacttg tacaattacc aatataatag caggtgaaca atatctatct
    10921 atagttatac caggaagaat gtataataag gaatataaaa agagaggtat acatcctaag
    10981 atgttgtcga gagctttaga agatagtgga actttaactt ctccattagt accatggaat
    11041 acatgcgggg cgtatataac agcaacatta ggtgtatctg cactaacata tggtccatac
    11101 gctttattaa atattataaa tccaatagta tctttagtat taattgcatg taagtttaaa
    11161 atagcaagaa tagaagatga acctgaaact tgtttaaatt ttgatgtttg ataatagata
    11221 acttatgtaa gaacgtagga actagggggg tagcctgtgg ggaattagta agacatattt
    11281 tagtgtgcga aattctataa atcagtaatc ttgtgacttt agccatggga ggttcagatt
    11341 agtaaattta tagttttata ttagtctata ttttgatatt ttaattgttt gtataaattt
    11401 tttatagaaa aagatttctt ttagacttgc ctaaaaatta ggcaagtctt ttaagtatgt
    11461 tctgattata agagcactgt gtgacaagaa tgatgagatt atttttgcta tactttcgat
    11521 ataagaaatg gaaggaggtt atgttttata ttcatatgaa ataattagtt acacagtgaa
    11581 ttttcgtata atttggtata aaaagattag aaatgaaaat aaagtaatgg aggaataaaa
    11641 tgactagaat atccaatata acaatagctg aacaatctga gtactgtttt ttggcaatac
    11701 gaaaaacaat tgactttatg gtagaatttt cagagttttc aaaacaaagt tttgaaaaaa
    11761 tttctaaata tctggaggag agaggaattt tatcttcagg agcaccgatt gtatgttttc
    11821 ataacatgga tttagtaaaa ttggatgttg aagtagggtt tcctgtagcc acttatatag
    11881 atgggaaaaa tgagattcta caaaagattg ttcctgcaca gaaaataata actgcaattg
    11941 atttaggact ttatgaatta caagacccta ctttagaaga cttattttca tgggcaaata
    12001 gcaatggata taaacttcat ggcgatatat attaccagta tttaaatgat atcaatcgtc
    12061 cagctagtga atatcttacg aaaatgatgt tacccattat atagaaagtt aaagattatc
    12121 gtttgttagt tgataattag aagaacattt actgctacta cataaatttt atcctaaata
    12181 atatcaaggt gatttaattt atgtattcta atgtaacccc aatatattgt taaggtctac
    12241 ttaagatgct caaaaggcta tgcttaattt attgttatat aatttgtaga gtggtataat
    12301 ttagaggtaa tattatatag atatataggg ggaaaatatg aaaatatatg atatattaac
    12361 acgtttttat attcaagata tagaaaaagc catacctttt tatgagaatc ttttaaaaga
    12421 aaaatgttct ttgcgttttt cctataaaga agttgggttg gaattagcgc aaataggaaa
    12481 tgttttacta ttatctggtt ctgatgatgc tcttaaacca tttatagaaa caaaatctac
    12541 ttttatggtt gattctgttg atgaatggag aagttatcta ttggataatg gagcagttgt
    12601 agtaagagat aagaaaaaag ttcctacagg atataatatg acattgagac atccagatgg
    12661 tacaattata gaatatgttc aacatacaaa gcaggattag ttaaaaacag attttactac
    12721 tgagcaacta attagaatct attcttagac gtacaattta ttatttttgg tattaataga
    12781 ttataagtca aattataata tttcaaaata gaatagaaat ttatttacat aaaagagagt
    12841 gtttaaaaat gaacttttta gttcatgaag cactcttttt tgtatgaaat caaatacatc
    12901 aatatataaa ataaactgat gaagagctta aattaaaata aaaagattca taacaggacc
    12961 ccattaaact ttaagatagg gtaagtttgt aatcatatta aataattgta aaaagctgtt
    13021 tttagtcgta taatataaat atttatcatt taattaagga atctaataag aagaaaataa
    13081 taaataactt tttagattta ctaaatgaaa agttattgaa aattaaagga ttagagtctt
    13141 taatcagaaa gtaaaaagta atatattaaa tttatgaatt gaaaacttaa acaacaaatt
    13201 tgtttataaa atagcaagat tcattaaata taactaagta ataattgata taatgttgca
    13261 tggataaaag gagggaagct gcaatgtctt acaaagtaat taatgtcata actgctattg
    13321 actatataga ggagcattta tcagaaaagc tggatttaga tatagttgct aatgctgttc
    13381 attactcaaa atatcacctg catagaactt tcacaacttc tgttggccta acaatgcatg
    13441 attacataaa gcggagaaag ttgacagaag cagcaaaatt attggtattt tcaaagaaac
    13501 caattattga aattgcattg atagctggat atgaaagtca acaggcattc acttctatat
    13561 ttaaagctat gtataaaaaa tctccaaata aatataggaa agaacaagaa ttttatcctt
    13621 tgcagctaag atttgttttg aaaaaagata acctattttc aaagaatgta ctagagttgg
    13681 aaaaagaaat taaacttgca agtatgtctg acataccatt atggatgaat ttagtgagat
    13741 tagtcataga tggatttccc aatttgcaag aggaagaata tatatatcaa ttagaacagt
    13801 atattttaga agaaagagct ttaatattaa aacttaataa tgtcgctatt gcaaatatgg
    13861 tgtttaatag agaaactaga agtattgatt tttttggaat acatccacag tatcgtaatt
    13921 cagacattgc acaagtgttt ttgaagaaag ttattgaaga tttcttgata gatacagata
    13981 tttcaattac tacctttcga gagggtgaca aagctgatac aggacataga gatatgataa
    14041 agaagttggg ctttgcagaa gcagaattgc ttgtggaatt tggatatcct actcaaaaat
    14101 ttattcttcc tttaagttaa atagcaagaa tgattaaaaa ctccaaaaca caattaagta
    14161 taatgattca gtgtaagtac ttaattaaca aaggaggatt taaatgtctg aaaataaaca
    14221 agtagtaaat tgcgatatta ttgcatctaa ttctaaacat aatatgtgtg aatatgatgc
    14281 agagttatta actttttttc cagatgagat tgcacatcta atagagataa ataacaagct
    14341 agacaatgct tttaagaaag ctgaaaatct tgttgacaag cttgataagg attatatgga
    14401 tgctaaaatg tatatggtaa aaaatcgtgg tgaaatagac ccacatgaaa tgtttcaaaa
    14461 tgaacaagga ctaaaacaaa tagataacta tggggctttt atggtaaaag ttcgagataa
    14521 gattaataaa atcaaagatt ctccatactt tgctagaata gattttaggt tgaaagatat
    14581 ggatgatgaa tctaagtatt atattggtag atttgcattt gattatgaag atgagcttat
    14641 aatacttgac tggcgttctc caattgccag tatgttttat gattatgaaa tcggaaaagc
    14701 tggatatgat gctccaatag gatgggtgga tggagaaata acaagaaaac gacaatttaa
    14761 aattaaaaat ggaaaattag aatatgcact agaaagttcc attaatatac aagatgatat
    14821 tcttcaaaaa gaacttagtc acacttcaga tgaaaaaatg aagtcaatca tttcaacaat
    14881 acaaaaggaa caaaatcaaa ttattagaaa tgataaagct gatacactga ttattcaagg
    14941 ggtagctggt tctgggaaaa catctatagc attacacaga attgcttttc ttctttatcg
    15001 atttaaagat aaaatatcag caaataatgt aattatccta tcacctaata aagtctttgg
    15061 agactatatt tccaatgtcc ttcctgagct tggagaagag ccattatgtg aattaagttt
    15121 tgaaaatata gcagaggtac aattagatag agttattaat tttgaaagtg agaaagaccc
    15181 attagaaata aatgatgcta aatggtctga aagagtgcgt tttaaatcta cacttgattt
    15241 tgtaaaactt attgatgatt atataaaaca aatgccaaat aagattttta ttccaaaaga
    15301 ctacactttt ggaagtttta cagctaaaag tgactggata cagagtagat ttgaggcata
    15361 taacagatat ccagttaaga agagattaga gaaggtagct gaagacattc attataaatt
    15421 tgaatcagat aatattatgg aagaagattt accaaaggta aaaagtatac ttaaaagttt
    15481 gaatggaatg ttgacaataa aaaatactct tacactatat aaggatttct ttaaacaaat
    15541 gaatatatca aacatgtttg tgatggcaga aaagaaaaca cttgaatggt cagatgtata
    15601 cccatttata tatattcatg ctgcatatga aggtatacaa gaagataaga ttataaggca
    15661 tattgttatt gatgaaatgc aggactatac acctattcaa tatgctgtaa taaacttact
    15721 ttttaagtgt aaaaagacta ttcttggaga ttttggacaa ttggttaatc ctaatcatac
    15781 tcatacacta gatgatatga aacagctata taatgatggg gaattagtta tgttgaacaa
    15841 gagttaccgt tctacttttg agattattaa tttcgccaaa aagattcaag acatatcttc
    15901 attagagcct attgaacgac atggtgaaga accagcttta ttaaaatgta ataacgagca
    15961 agatgaaata aataagataa aaatagaaat tgaagaattt aaaaagagtg ataatgcgac
    16021 tcttggtatt atattaaaga cagataatga cgctaaagct gtatatgatg cgttaagaga
    16081 agaatatagt gttcacttaa tatcttctga aagttctagc tttacaaaag gagtatccat
    16141 aacttctatt aaaatgtcaa aaggtttaga gtttgatgaa gttattgtac cttcagtaaa
    16201 taataaaaca tattatagtg attatgaccg cagtttgtta tatattgcct gtacacgagc
    16261 tatgcataaa cttaagttga catatacttg tgaattgact caacttatag atatgtagat
    16321 tatatactta ttatgtaagt gtttacttat ataaatataa cctgtcaata aaattcatta
    16381 ataaatcaat aaaaacaata tttgtaaaag taaaagctat aaattatata ttaaatttta
    16441 taatttatag cttttataat actgcttatc ttaatcctcc caatagcaaa ataattttaa
    16501 acatgccata ttttataaat gtaataacaa ttatacaatt aaaataactt tcctaaacta
    16561 acttgacatt taaaacttac tattgtaata tttaaatatt acaatagtaa gttttaaat
//
LOCUS       DACULQ010000081        16507 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.081, whole genome shotgun sequence.
ACCESSION   DACULQ010000081 DACULQ010000000
VERSION     DACULQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.081"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            213..1208
                     /locus_tag="JA154_10455"
     CDS             213..1208
                     /locus_tag="JA154_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HAT7996284.1"
                     /translation="MFITVVLFLVGFLLITKGADIFINCTVEIGKKTNISEIILGATI
                     VSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICNTGLALGLVVFISPFNVDKKMFF
                     SKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIFYMVNNYRSVVGKSDTKNRNIKN
                     NTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMMIIGSQILIESGVIIASFLNIPQ
                     GIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNILGANILNIVSVIAVSAIPNNIP
                     ILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGILMLFTYFLYISILYFMYII"
     gene            1299..2912
                     /locus_tag="JA154_10460"
     CDS             1299..2912
                     /locus_tag="JA154_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAT7996285.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            3187..5172
                     /locus_tag="JA154_10465"
     CDS             3187..5172
                     /locus_tag="JA154_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein BlgG"
                     /protein_id="HAT7996286.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            5177..5611
                     /locus_tag="JA154_10470"
     CDS             5177..5611
                     /locus_tag="JA154_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7996287.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            5640..6200
                     /gene="srlA"
                     /locus_tag="JA154_10475"
     CDS             5640..6200
                     /gene="srlA"
                     /locus_tag="JA154_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087241.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAT7996288.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            6264..6632
                     /locus_tag="JA154_10480"
     CDS             6264..6632
                     /locus_tag="JA154_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HAT7996289.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            6657..7223
                     /locus_tag="JA154_10485"
     CDS             6657..7223
                     /locus_tag="JA154_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HAT7996290.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            7264..7629
                     /locus_tag="JA154_10490"
     CDS             7264..7629
                     /locus_tag="JA154_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HAT7996291.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            7730..8503
                     /locus_tag="JA154_10495"
     CDS             7730..8503
                     /locus_tag="JA154_10495"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087245.1"
                     /note="catalyzes the conversion of sorbitol 6-phosphate
                     into fructose 6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate 2-dehydrogenase"
                     /protein_id="HAT7996292.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            8852..11290
                     /locus_tag="JA154_10500"
     CDS             8852..11290
                     /locus_tag="JA154_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HAT7996293.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDMALHKVNYVSDELDTTSNKVNYVSDELDMALHKVNGVSDELDMA
                     LHKVNCVSDELDMTSNKVNYVSDELDMTSNKVNYVLDELDITSNKTDYISNELYNLSN
                     KSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTVTLCDMFIP
                     CTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVEKR
                     TYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKNLG
                     ISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINQRMAKKVG
                     IEEKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFGGQ
                     VTYNESMVEIIRQN"
     gene            11378..11713
                     /locus_tag="JA154_10505"
     CDS             11378..11713
                     /locus_tag="JA154_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HAT7996294.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            11727..12155
                     /locus_tag="JA154_10510"
     CDS             11727..12155
                     /locus_tag="JA154_10510"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087248.1"
                     /note="binds to sigma F preventing its association with
                     RNA polymerase during sporulation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HAT7996295.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            12178..12942
                     /locus_tag="JA154_10515"
     CDS             12178..12942
                     /locus_tag="JA154_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087249.1"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; this sigma factor is
                     responsible for the expression of sporulation specific
                     genes and is expressed after engulfment; this factor is
                     involved in the transcription of small acid-soluble
                     proteins involved in protecting the forespore chromatin;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-G factor"
                     /protein_id="HAT7996296.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            13222..13659
                     /locus_tag="JA154_10520"
     CDS             13222..13659
                     /locus_tag="JA154_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HAT7996297.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            13826..14839
                     /locus_tag="JA154_10525"
     CDS             13826..14839
                     /locus_tag="JA154_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HAT7996298.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            14853..15215
                     /locus_tag="JA154_10530"
     CDS             14853..15215
                     /locus_tag="JA154_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HAT7996299.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            15398..16414
                     /locus_tag="JA154_10535"
     CDS             15398..16414
                     /locus_tag="JA154_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAT7996300.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         6613 a         1673 c         3064 g         5157 t
ORIGIN      
        1 aaaaattgtc gctttaaagt ataaaacaga ttatatgtta ataatgtggt gaaattatac
       61 ccatatataa ataaaataaa acaaataaat agctaattaa ggagaaaata ttttttattt
      121 tttgtttagg ataaataaat tttattatgg aaaaaagaaa aagcatacat cttcacaaat
      181 tgaatattta tttaataggg ggaggtgata gattgtttat cacagtggta ttatttttag
      241 taggtttcct attaattact aaaggcgcag atatctttat aaattgcact gtagagatag
      301 gtaaaaaaac gaatatatca gaaattatac ttggagctac tatagttagt tttgcaacaa
      361 cattacctga atttacagtg tctctacttg cttctattga tggtcatact actatgagtt
      421 taggaaatgc agttggttct ataatatgta atacaggatt agcattaggg ttggttgttt
      481 ttataagtcc atttaatgta gataaaaaaa tgtttttttc taagtcttta ctattaatag
      541 tatccgttat agttttaatt ttattgagtt tagatggagt tataacaaga ggtgattcat
      601 tattacttat aataatatta atcttttata tggttaataa ttatagaagt gtcgttggaa
      661 aatctgatac taaaaataga aatattaaaa ataacactat taaagacata tctactaaaa
      721 aagggaaaaa ttatagaggt ttttccgtat tagaaattgt aaaaatacta ttactttttg
      781 ctacaggatt gataatgatg ataataggtt cacaaatcct tatagagagt ggagtaataa
      841 tagctagttt tttaaatata cctcaaggta tagtaagttt aactataata gcacttggaa
      901 cttctctgcc agagatagtt tcgtcgatta ctgctataag aaaaaatcat catgaaatat
      961 cagtaggaaa tattttgggt gcaaacatat tgaatatagt atcagtaata gctgtatcag
     1021 caataccaaa taacatacca atactatctc aaaatagaca attggatata ccattcatga
     1081 tattattatt attaatagta ataataccaa cacttaaatc taatagactt agtagaatac
     1141 aaggtatatt gatgttattc acatattttc tgtacatatc aattttgtat ttcatgtata
     1201 ttatttaact atactgttta aaatattaat tgcagaatat cctaaaaaag tatataatgg
     1261 ataggttaag attaattgat aacggagagg aataatatat gaatataaca aagtttgaag
     1321 atttaccaat aagtgaggga ataaaaaaag ctatagctga aatggggttt gaagaaccat
     1381 ctcctataca agcacagtct atacctgcga ttttgtcagg taaagacgtt ataggacaag
     1441 ctcaaactgg tacgggaaaa acagctgctt ttagtatccc tatactagaa acaatagacc
     1501 caaataatag aagtttacaa gctgtagtgc tttgtccaac aagagaactg gcaatacaag
     1561 tatcaacaga aataagaaag ttagctaagt attcgcacgg aataaagaca ttacctatat
     1621 atggtggtca gccaatagat agacaaataa aatctctaaa gagtggtgtg caagttgtaa
     1681 taggtacacc aggacgtaca atagaccata tcaatcgtaa aacattaaag atggataatg
     1741 taaaaatgat tatattagat gaagcagatg aaatgctaga tatgggattt agagaagata
     1801 tagaaatgat tttaagtaag attccagaag aaagacaaac aactttcttt tcagctacta
     1861 tgccaagagg aattttggaa ttaactaaga gatatcaaaa agacccagag catataaaag
     1921 ttgttagaaa agaacttaca gtttcaaata caaagcaata ctatatagaa actaggtcat
     1981 caaataaatt agaagtatta tgtagattag tagatgtata tgaccctaaa ctatctgttg
     2041 ttttctgtaa tactaagaga aaagcagacg aattggtagg agatttacaa gcaagaggat
     2101 attttgcaga tgcattacat ggtgacttaa aacagactca aagagatata gtaatggata
     2161 agtttagaaa tggtacaata gatatacttg ttgctactga tgttgcagct agaggtatag
     2221 atgttgatga tgttgaatgt gtattcaact atgacttacc acaagatgaa gagtattacg
     2281 ttcatagaat tggtagaact ggtcgtgctg gtagagaagg tatgtcattt acttttgtat
     2341 ttggaaaaga aatgagaaag atgaaagata tagaaagata tactaaatct aaactaataa
     2401 agcataatat accaacaata actgatgttg aagagaaaaa agtaggtact ttctttgcac
     2461 aagttaaaca aactatagaa gaaggtcact taactaaaca attacaatgg ttagaaggtt
     2521 tctgtaatga tgaagattat gcaatggttg atatagctgc tgcacttgta aaactatctc
     2581 taggtgaaga aatgaaagaa gaaataatag aagaaaaacc tagaagagag cgtggagata
     2641 gaaaaggtgg tactggagct aaagatggta tgattagatt atttatcaat attggtagaa
     2701 atcaaagagt acaagcaaaa gatatagttg gtgccatagc tggagaagtt ggaataccag
     2761 gtaaagtagt aggtactata gatatatatg ataagtatac ttttgttgaa atacctaaaa
     2821 aagatgcaaa aacagtaatt gaaaagatga aagatattaa gataaaaggt aataagatta
     2881 atatagaaaa agcaaacaag aagaaaaaat aacgtatagt atacaaaact ataattagaa
     2941 aatatataaa ataatatatt ggatatttaa aaagcctgat gtgttttcaa aacagatagt
     3001 agataatctg taagagttaa cagcataagg cttttcttgt tttatatgat gttataattt
     3061 tccagattgt tgtgaaaaat gtatattagt tccttttttt caaatctgat ttgaaaaaga
     3121 gaatattaat tatctatata gtttagatta taataaaaaa tgtaagataa caaaggggag
     3181 aaaaagatgt caatgactta tatggaccct agggtttttt tgattttaga aatggtgtta
     3241 gaagaagaca caaatactct agaacaattt tcaaatgttt tgggtgttag tactaggact
     3301 atcagaaact atataaacca gattaatcga gaattaaaag gtggtattgc taaaatagtt
     3361 aaaaatgaaa aaggtacata tagtataaga attgaggatg aaagtaaatt aattgagatt
     3421 gtaaatttta atagaggaaa gctatcaaat tttataaatt taaattcacc tgatgaaaga
     3481 ataaattatg ttcttgatat tttaactatg accaacaatc caattaccat agatgatttg
     3541 gctgaagaga tatgtatagg aagaacaaca ttaataaagg atttaaaaaa agcagacaga
     3601 attttagctg aatatgattt agaattaaaa aggaagccaa atataggtat gattttagtt
     3661 ggtgaagaaa tggatataag attattaatc cttgatagat tgtatgaaaa ttatattgat
     3721 gtcttggaaa acgttaatca gataaattta gttaaagatt atgatattga atgtcttaga
     3781 gatgaattaa aaaagttatt taaaaaagaa gaactctaca taacagaaca ggtcttgaga
     3841 gatgttgaaa gatatataat catgtctgtc ttaagaaatc taaatgggta caaatttgaa
     3901 aagatagata agagatttga agtgataaga tgttcagatg aatatacttt aggattaaag
     3961 ctcaaagctt tattggaaaa gatatttaat atagttttaa atgaaaaaga aaccatattt
     4021 ttgacaatgc cattaatagg tagaaaagct cctagtacaa aattagcact tagcagtata
     4081 aagataaatg atagcgtaaa ggaagttgta gatgaagtaa ctagtttttt attagagacc
     4141 tcagggattg attttaaaga agataaaaag cttatagaaa atttggaata tcacttgtat
     4201 tttgcactta atagaatgag attcaacata agggtaaaaa atcctctttt acaagaaata
     4261 aaggaaagat atacatttcc atatagtgtt gcaaagatag ctgcaatgat aattgaggaa
     4321 aagtttgact tgaaaatatg tgaccacgaa ataggatata tagcattaca ttttggaagt
     4381 tattttgaaa aaaattcaag aaaagttcta aatgtaaata gggtagctgt tgtttgtgga
     4441 acaggtcttg ggactgcaca acttattaga ataaagatag gaaaaatact tggagaaagt
     4501 gtagaagtaa atactttttc tgatatagaa gctaaagcta atttagattt attaaagtat
     4561 tatgatttag tatttacaac agtagatttc aaaacagact tagatgttcc aatttttaga
     4621 gtaaatgcgt tatttaatga agaaagtata aagaaagaga tagaaaatgc aatacttttg
     4681 aaaaatagta taaatcttaa gtctataaaa aagattgaaa aaaatatccc ttttataggt
     4741 aaattaattg aagaggataa gttctttata ttgaataaga gtacgtttat ggaaaattta
     4801 gaagatatgt tagaaaaatt agttcataaa ggaattattg atatagagtt caaaaaaaag
     4861 gtattaaaaa gagaaaagaa atcttcaaca gcattagata attatatagc attgcctcac
     4921 tcagttaata ctaatgggga aaatctttat ctggcatttg gaatattaga aaaaccagtg
     4981 atttgggata caaaggaaat tagaataata atattgatga taattccaga taagaatgta
     5041 gatagcacag acctgataat aaaaagttat gaagaagtac ttgaagtagg gagagataaa
     5101 aagatggtag aagaacttgc taaggtaaaa tcatttgatg aattttataa aataattacc
     5161 aagaggaggt aataatatgg acccagtaat ttttattata gcatcactta ttacagtgta
     5221 tttattaaat atttttcttg gatacttaca gcttaaagat tttaataaaa attatattga
     5281 actaaaaaga aaaggaagag tggcaattgg cagaaaaaaa ggaagaattt catcaggtac
     5341 aattgtgttg atacttataa atgatgatgg tgtgattgtc gagactagaa aaatgcaagg
     5401 tgttacagta ttggcaagag taaagtcatt tgaaggtttg gtaggaaaga gcttagagta
     5461 tatagaaaaa tcagatttaa ctgtttacaa taaactactt aaaaaagcaa tactagatgc
     5521 agtcaaacag tacataacat ttgagaaaaa taaatgtgaa gatacaaata atctgagtac
     5581 tgatgaagag tgtaggatgg aagtagttta aaaaaatata tttataagga ggagttaata
     5641 tgacgcaatt ttttcaagta ttagcaagtg gtgcagatag ttttatgaat ttatttaggg
     5701 caggtgggca acaatttgta ggttttgtaa cagatatagt accactttta gtagcattat
     5761 tagttacaat gaatgcaatt atcaacttta ttggtacaga tagagtcgag agacttgcaa
     5821 aaaaatgttc tgcaaatata tttactagat atttgatatt accagtcctt ggtacatttg
     5881 tatttgcaaa cccaatgaca ctatcattag gaagattctt gccagaaaag tttaaaccta
     5941 gttactttgc agcagcaagc ttttcatgcc atacaatgaa tggattattc ccacatatca
     6001 atccaggtga actatttata tatctaggaa ttgcaaatgg tataacaact ttgggattct
     6061 caacagcaga tttagcagtt agatatttgt tagtgggtat agttgcaaac tttattaaag
     6121 gtgtagttac tgactataca acaaaatttg tagagaaaca acaaggaata gcattaaatt
     6181 cagatataaa gattggataa ttactttaag gtataaataa atttttcaaa taaaaagata
     6241 gtgtatagag gaggataaat tatatggcat ttaacaaagt taaaatagta aaaggttctg
     6301 gaggatgggg tggaccattg ataatagaac caacagaaaa gaagaataag gtagtatatg
     6361 tgactggggg agcaaagcca gaaactgcag taaaaatagc tgaacttaca ggatgtgagt
     6421 taatagatgg attttcacag ggtgttaaag atgatgaaat cgcatgtgta ataataaatt
     6481 gtggtggaac attgagatgt ggtatatatc ctcaaaagaa aataccgact ataaatataa
     6541 tgaaaacagg aaaaagtgga ccattagcaa tgtttataaa agaagatata tatgtttcag
     6601 gggtaaaacc aacaaatgta gagcagttat aattgaggta gtaggaggga agaaatatgg
     6661 cttatgatac taaaaagaag ataagtgagc agagtcaagg aattgttgca aaaataggta
     6721 tagcactagg aaatgtgaca agtactcttt ttcaagctgg tagggatact atagatacat
     6781 taatacatac tatactgcct tttatggctt tcgttgcagt cctaataggt ataataaatc
     6841 aatcgggtat aggaaattgg gtagccaatt tacttactcc attaatcgga aatgttggag
     6901 gtttagttgt cttatcaata atatgttcat ttccgttgtt gtctccgttt cttggaccag
     6961 gagctgttat agctcagata gtgggggtat tggttggtac agaaatagga aaaggaaata
     7021 ttccaccaag tttagcatta ccagcattgt ttgcaataaa ctcacaagca gcttgtgact
     7081 ttataccagt aggtcttggt cttgcagaag ctgaacctga tacagttcaa gtaggagttc
     7141 cgtcagtttt atattctaga tttttaacag gtgcaccaac tgttctatta gcatggttag
     7201 caagctttgg gttatataaa tagaagaata ctttataaat aattagagag aggttcagtt
     7261 gatatggaat taatatacag tactcaaata aataaaatag gagataatgc aaatgaattt
     7321 ttacaacata atatgtttgt aactttcaaa gataatgctc ctcaagagtt agaaaattac
     7381 tgttatatac attctgaaaa taatttagta aaagaaatac ttacggatga tgtgctatca
     7441 ataaataatg ttgattacaa aattacttct gttggtgagt tagtaaacca aaatctttca
     7501 gaattaggtc atataacatt taagtttaca ggtgaaaaag atggagcaat tggaggaacc
     7561 ttatatcttg aagaaaaaga aatagcacct attggcgtag gtactatact aaaaataatt
     7621 agaaaataaa atttaaaacg aatctaatca tattataagt aaattataga taaaagtcaa
     7681 tttaactaat caagttaact tttaatgtga taaaaaaggg gaaatgaaaa tgagaaaagt
     7741 ggctttagtc gttggaggag gaagaagttt aggtgaatat ttatctaaac atttagctaa
     7801 agtaggttat gatgttgcta ttgcagattt aaatgaagac aacgcaaaaa aagtagctga
     7861 agaaataaaa actgaatttg gttgtaaatc tgtaggatat agttgtaatg ccatagatga
     7921 aagacaggtt ataagtactg tcaaagctat tgaaaatgat tttgatagaa tagatttatt
     7981 agtgtacaat gcaggagtgg ctgtaagtaa aaaaataact gattttggat acaaagattt
     8041 taaatttatt gtagacgtaa atttaaatgg gtactttcta tttgcaagag aagtatctaa
     8101 agtcatgata gcaaaaggta ttgagggtag aataatacag ataaattcaa aatcaggtag
     8161 agtgggaagc aagcataatt caggatattc agcaggtaaa tttgctggag taggacttac
     8221 tcaaagttta gcgctagatt tggcagaaca taagataact gtaaatgcac tgatgctcgg
     8281 taatttactt gattctgata tgtttgaaag tttaattcct caatatgcag taaaattggg
     8341 aatacctgaa tcagaagtca agcaagttta tatagataaa gtacctttaa aaagagggtg
     8401 tagatttata gatgttgcaa atatgcttac attttatgct tcaaaggaag ctgaatactg
     8461 cactggtcaa tctataaata ttactggtgg acaagttatg taaggttttt atggagtgtc
     8521 tcaagatgaa ctctttagtt catgaggcac ttttttgtat aaaattaaat atattttcag
     8581 catttaaaca atgaaaaaga ggtttatctt tattttgaga tagcctcatt tttgtaatta
     8641 aaaaacatag tgtttaagta aacatataag attgttagtt ttttaaaaga gaatttttta
     8701 ataatattat atttatgaag tatttttaaa cttaatttga taaaatatag ctatgtttta
     8761 tatactgtag ttttgaacat agaaagttta ataaattata gtatatatgt taatttttaa
     8821 taaggtaaga aagtagaaag aaggatattt tatggatata ataaaaaaat taagtcatgg
     8881 gtgtacatta gattgtcatg actgttgtaa attcaatgtt tatactaaag gaaataatgt
     8941 agtaaaaatt gaaggagata aaaatcatcc ttatacaaaa ggttttatat gcaaaaaagg
     9001 gatggcccat ttagatagat taaatcataa agatagaata aagactccta tgcttaaagt
     9061 tgatggagta tggaaagaga tttcttttga taaagctata gaaataatgg cagaaaagct
     9121 tacatactat aaagaaaagt atacatcaaa gtctgtcatg cattatgacc aatatggaag
     9181 tgggtctgtg ctaaaataca taggagatat attttttaat ttttatggag gtgttagcag
     9241 acataagggt ggtccctgtt ggagtgcagg gatgcatgct caaaaatatg attttggagt
     9301 agctaagagt catgctatag aagatatgtt aaatagtaaa agtatatttg tttggggaaa
     9361 aaatccagca tatactacaa ttcatactat gcaaataatt aaaaaagcta aagaaaaagg
     9421 gataaagata gtagtgattg accccatata tacaaaaaca gctcaaatag cagataagta
     9481 tgtacaagta aatcctggaa cagatggagc tttagctata gctatggcaa aaattatagt
     9541 ggaagataaa ttatatgatg aagaatatat aaactcgtat gttattggtt ttgaagaata
     9601 taaaaaatat ttatcttcat tggaattaag ttttttaatt gatgaatgtg gtgttaaaga
     9661 gaatgatata agagagttag tggatttata tactaacaaa tattcaagta taaatgttgg
     9721 atatgggctt caaaaatata aaaatggtgg aaacacaata agagcaatag atgctttagg
     9781 tgctataact ggtcaaatag gatttagtgg tggaggggta aactatgcta ataaagttta
     9841 tccgagtgtt ataaattctg acccttacaa tagccaatca tatggtgaag atagggagtt
     9901 ttatgtaagt aatatcagta aatttataga agaatcttta aaaaatacat caaataaagt
     9961 aaattatgca tcagatgaac ttgatatggc attacataaa gtaaattaca tatcagatga
    10021 acttgataca acatcaaata aagtaaatta tgtatcagat gaacttgata tggcattaca
    10081 taaagtaaat tatgtgtcag atgaacttga tacaacatca aataaagtaa attatgtatc
    10141 agatgaactt gatatggcat tacataaagt aaatggtgta tcagatgaac ttgatatggc
    10201 attacataaa gtaaattgtg tatcagatga acttgatatg acatcaaata aagtaaatta
    10261 tgtatcagat gaacttgata tgacatcaaa taaagtaaat tatgtattag atgaacttga
    10321 tataacatca aataaaacag attatatatc aaatgaattg tataatttat ctaataagtc
    10381 aatcaaagat aatattccta taaagatggc tgtgataact aaaagtaata tgctgaatca
    10441 actgccagat ttagtagaat tggagagggt gttttcaaaa attgaattta aagtatgttt
    10501 tgatatgttt ataactgata cagttacttt atgtgacatg tttataccgt gtacaaatac
    10561 actggagagt gaagatataa tttatagctc aatgaccaat ccatatataa cctataatga
    10621 aagagctgta aagccagcac ataaattgat ggatgaatat tacttcttta tggagctagc
    10681 taaaaagatg ggattaaatg attatccttt tgtagaaaag agaacttatt tagaaaaagt
    10741 gattgaacca cttaagcgtt ttgacaaaaa tctggatata gaaaaactta aaaacaatta
    10801 ctttactata cataaccctg ttgcttggga agataagaag tttgagactc catctggaaa
    10861 atacgagctt tattcagaaa gtataaaaaa cttgggaata agtccaacac ctgtatatat
    10921 aagcaataaa tataaagaaa tagaagataa aaatatttct tttagattat taactaatca
    10981 tcatgcagat actttattta gtcagcattt tatggacaaa aaatctatag ctcaagcata
    11041 tataaatcag agaatggcta aaaaagtagg tattgaggag aaagatattg tgatattgag
    11101 gtcaaaaaaa gcaaaaataa atgtacaaat aaatatagat gatggagtag gtaattatat
    11161 agtaaaaatg tatgttggtt ggtggaagaa acatggaaat cctaatagtt taacagacac
    11221 tggtatatca gattttgggg gtcaggtaac atataatgaa agtatggtag aaataatacg
    11281 acaaaactag ttaaatttca ttgaaggaat aaaaatataa ttatagaatt gattaaaagt
    11341 tactattttt acaaaactat tgaaggaggg atttttgatg gtaaattatt ccttagaaca
    11401 taagaatcta tacatagaat ttatgtgctc agaactagac caccatgtag ccaatgaaat
    11461 aagggaagaa atagataact tattaagtgt aaatcaagta aaaaatgttg tatttaattt
    11521 tgaaaatatt aattttatgg attcttcagg tataggcgtc atcataggta gatataaaaa
    11581 gatttctaat gaagggggac gagtttcagt aattaatata agttcacgag ttaagaagat
    11641 ttttgattta tctgggttaa ataaaattat tggcatttat gatacgtatg aagaagctct
    11701 aagttctttg tagggaggag tatgtgatga ataatattat ggaagttaaa ttttcggcaa
    11761 tatctgaaaa tgaaagttta gcaagagtta tagtagcatc ctttgcagct aagttagacc
    11821 caacagtaga tgagttagta gatataaaaa ctgctgtctc tgaggctgtt acgaatgcta
    11881 taatacatgg atatgaagaa gacagttcta agtttgtttt cttaagatgt gagatagaag
    11941 gaaatactat aaagattata gttgaagatg aagggtatgg aatagaaaat gtggagaagg
    12001 caatggaacc actttataca tcaaaaccag aacttgatag atcaggaatg ggattcacag
    12061 taatgaaaag ctttatggac gatgtagagg taagttctgt aaaagataat gggactagaa
    12121 ttgaaatgac taaaaaaata aatgtgccta aataaagtcc aaaagtgagg ttttattatg
    12181 gaagtaactg ttgccagaga agaaaaaaaa cctcttctta gtcatgaaga gactctagaa
    12241 cttattgaaa aagttcaaaa tggagatgaa gaggcaaaag aaatattaat atcaagcaat
    12301 ttaggtctag ttaggagcgt tgtatctaga tttttaaata taggatatga tagagaagat
    12361 ttatttcagt taggttctat aggcttaata aaatcaatat ataaatttga cccaaaattt
    12421 aatgtaaaat tctctactta tgcagtacca atgattttag gtgaaataaa gagatactta
    12481 agagatgatg gtatgataaa agtatcaagg tcgttaaagc aaatagctgt aaaggcaaag
    12541 atggaaagtg aagctttaac taaaaaatta ggtagagagc cgagtataga agaattagct
    12601 aaagcaatag atgtagaaaa agaagattta gtaatggcta tggaagctaa tttcaatgtt
    12661 gagtatttgc aaggagttat tcatgaagag gaaggttctc caatatgcct tattgataaa
    12721 ataagcatga aaggagaaag tgaagaagaa aaagtagtgg ataacatatt gttaaaagac
    12781 atacttggaa gactggataa gagagagcgt caaataatag ttctcagata ctttgaagac
    12841 atgactcaaa gtgaaatagg agagatgctc aatatatcac aagttcaagt atctaggata
    12901 gagaagaaag tattgtctaa gttaaaagaa tatatatcgt gataatactt atgatatgta
    12961 gaataacaaa atataataaa tatattacta aaataaaatg taataacttt ttatgttaag
    13021 tttttaaatt attaaaagat attaaaataa taaaaagcat taaaaaagta aaaatataaa
    13081 aacaaggtgt ctttatggaa aaatagattt tttctttatt aggcatcttt tttatttttt
    13141 gaccaaactt tagatagtca aaataaataa taggtttatt tttttaaaat taacaaataa
    13201 tactttagag aggtgtttag tatggataaa aattataaaa aatatgtaga ccaaataagc
    13261 ccaaaaccta catatttaaa aaactataca cttgcattta ttgtaggtgg aattatttgt
    13321 atgatagggc aagcaataaa tgatttgtat atgaaagttg gaggtcttga taaactaggt
    13381 gctagttctg caacatcaat aacattaata tttatagggg catttttaac tggattagga
    13441 gtttacgacc taataggaaa aagagctgga gctggttcta taattccaat aacaggtttt
    13501 gctaactcaa tagtatctcc agcaatggag tataaaagag aaggctatgt attaggtgta
    13561 ggtgcaaact tatttaaaat tgcaggacca gttttagttt atggtatagg ttcttctatt
    13621 ttatgtggaa ttatttatta tatttttaag atgttttaga tgatttttat ttaatttaaa
    13681 ttttattaat tatgaagaag gtataaaatc tgaaaatagc tactaaagta actaattgaa
    13741 aaataattgt gcagatttaa ttaaaatttt taattaatat taaaaatttg aatagtaaaa
    13801 tgcaaaatta aaaaggtgtg aaaatatgaa aaataaaaga attggaaaaa gaacagtcaa
    13861 gttagaaaat aaaccaacca taatatcaac tggaactata gtaggtccta aggaaggtga
    13921 aggaccacta aaggactatt ttgatatgat tatgactgac gatttatatg gtgaaaagac
    13981 ttgggaatta gctgaaagta aaatggtaga gactgcatct caacaagcta tccaaaaggc
    14041 aggtaaaaaa ttgtcagatg taaattatat gctaggtggc gatttaataa atcaaatagt
    14101 tccagcatca tttgcagcaa gagaactggc tattccattt ttaggtatat atggagcttg
    14161 ttctacaatg gcagaaggtc tttgtatagg ttctatgctt atagatggtg gttttgctga
    14221 tttagtactg tcaggtacat caagtcacta ttgtacagca gaaagacaat ttagattccc
    14281 acttgaactt ggaaatcaaa aaccaatgac agcccagtgg acagttacag gagcaggaag
    14341 tgtattatta gctcctaatg gagacggtcc aaaagtaaag tatgttactg ttggtaaagt
    14401 aatagatgag ggtattgatg atggtaataa tatgggacca gctatggctc cagcagcaat
    14461 agatactatc tactcttact ttgaagatac taatgatgac ccaaatagct ttgatataat
    14521 tgctactgga gatttaggta ctcttggaaa acagatagca gaagactttc taaaagaaaa
    14581 aggtgtagat atatcaaaag tttatactga ctgtggtata gagatgttta accttaaaga
    14641 acaagatgtc cactgtggtg gaagtggatg tgggtgttca gccacagtgt ttgcaggata
    14701 tatatatgat aagttgagaa aaaaagaatt taacaaagta atgcttgtat caacaggagc
    14761 actactttca ccgacaagta cactacaaaa gcagacaata ccatgtgttg cacatgcagt
    14821 agtaatagta aatgagtagg aagtgatata atatgttaat ggactatgta agagtattta
    14881 ttgtaggtgg aattatatgt ttaatagccc aagtaatgat ggattatttc aaaatgcaaa
    14941 ctccatatgt tatggtaacg tatgtaacaa ctggtgtagt tttaactttt ctaggacttt
    15001 atgagccatt agttaagttt gcagcagcag gtgcaacagt tcctataata ggatttggat
    15061 attcacttgc tacaggagta ataaaatcaa ttcaaagtga tggtttttta ggtatttttt
    15121 taggaggtac aactgctaca gctggtggaa tagcagcagc tatattcttt ggatatatta
    15181 tgtcagtagt atttactcca aaagcaaaac cataaatctt tattaaaaga ataataagtt
    15241 tttgaaataa taaattttta taaagcaggt ggatatcttt tagattccac ctgtttttta
    15301 tataaagaag cgtttacata attgaagttg acaattattt acaacatagt gtagtattat
    15361 attatcaaaa taaaatgaag tgaagtaggg gataaagatg gtaactatta aagatattgc
    15421 aaggaatcta ggtatcagtt attcaactgt ttctaggtgc ttaaacaata atcctaatgt
    15481 atctgaaaaa acaaaaaaga aagtggtaga agaggcaaat agactagggt tccattttaa
    15541 tgtaaatgca agaaatttag caaaaaaaga aactaacagg ataggggtaa tcttttcaaa
    15601 taattttaat catcaagata ctagaaagtt ttttagtgat ataatggata gttctataaa
    15661 ttctattgag actaataaat acgattttat aatacagcct aataataaca ttagtggtga
    15721 tagtaatgta tataaaatgg taaatggaca aatggttgac ggattagtaa ttgtgtctca
    15781 atcaataaaa aaagaagagt atgatttttt aaaagacaat aattttccac atgtatttat
    15841 atattttaaa ccatctttta taggagaagt agataatttc ttttgggacg ataacgtgta
    15901 tggaggttat atagctacca agcatttaat tgaccatggt cataaagata taataacagt
    15961 aacttcagat gataagagtt caaaaatgca tgaagataga accaagggat atttgaatgc
    16021 tatggaagaa gcaaatttaa agacagaagt cattaaatct aaaatggatt ttgaatctca
    16081 agttgaattt ttagaaaaat acattgataa aataaaaaaa gcttcagcta tatttgtaca
    16141 acaagatgta ccagcgattt ctataataca agagttaaaa actacttatg gtataaatgt
    16201 accagaagat atttccataa tagggtataa taatattgaa ttaatatcat attttaggtc
    16261 gcatttatca acaatagatg acccaagaga gcaagttata aaaaatggag tagatagttt
    16321 agtaaatata ataaataaaa agagtacaga acacattccg agaaaattat atccaaggtt
    16381 aataatacga aatagcgtta aaagaataaa gtagtaagct tttaagtatc aaacgtttat
    16441 taaaaatatt atagattatg tgaagttttt taacaaataa tataaataaa aattaaaagg
    16501 agtaggt
//
LOCUS       DACULQ010000082        16365 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.082, whole genome shotgun sequence.
ACCESSION   DACULQ010000082 DACULQ010000000
VERSION     DACULQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.082"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..2089
                     /locus_tag="JA154_10540"
     CDS             <1..2089
                     /locus_tag="JA154_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAT7996301.1"
                     /translation="PNLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALE
                     LHDTNEELTKKTESLKEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFS
                     KDGEITYANKSFRNTFGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDN
                     KVYQAIFAPLIIASQTEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLE
                     GNNKIYVNNACREIFKNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAG
                     TNKTIIVIRDTTSRKISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDM
                     LAFTNIDDIKDSNIKLLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVE
                     VELSALPFAIYNTRYIMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELK
                     TPLNVISSSNQLVDSFYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMES
                     GFYTLKLAKYNIVSVIEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLN
                     LLSNCIKFTDNGGWIYVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSR
                     NTEGSGIGLAIVKNLVSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNI
                     QEKIKIEFSDIYY"
     gene            complement(2208..3152)
                     /locus_tag="JA154_10545"
     CDS             complement(2208..3152)
                     /locus_tag="JA154_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAT7996302.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            3316..4686
                     /locus_tag="JA154_10550"
     CDS             3316..4686
                     /locus_tag="JA154_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HAT7996303.1"
                     /translation="MIIMELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFG
                     KGVYDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSE
                     PFKLNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKC
                     IKHYYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDK
                     VYIKNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSE
                     NIDEKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYP
                     EILTIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDL
                     KNDVRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAII
                     TLCNKI"
     gene            4774..6663
                     /locus_tag="JA154_10555"
     CDS             4774..6663
                     /locus_tag="JA154_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT7996304.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            6678..7133
                     /locus_tag="JA154_10560"
     CDS             6678..7133
                     /locus_tag="JA154_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT7996305.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            7192..7509
                     /locus_tag="JA154_10565"
     CDS             7192..7509
                     /locus_tag="JA154_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAT7996306.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            7534..8796
                     /locus_tag="JA154_10570"
     CDS             7534..8796
                     /locus_tag="JA154_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAT7996307.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            8901..9965
                     /locus_tag="JA154_10575"
     CDS             8901..9965
                     /locus_tag="JA154_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAT7996308.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            10019..11197
                     /locus_tag="JA154_10580"
     CDS             10019..11197
                     /locus_tag="JA154_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAT7996309.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(11388..12446)
                     /locus_tag="JA154_10585"
     CDS             complement(11388..12446)
                     /locus_tag="JA154_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAT7996310.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSKIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            12513..12884
                     /locus_tag="JA154_10590"
     CDS             12513..12884
                     /locus_tag="JA154_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAT7996311.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIESRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            12921..13595
                     /locus_tag="JA154_10595"
     CDS             12921..13595
                     /locus_tag="JA154_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAT7996312.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            13844..14683
                     /locus_tag="JA154_10600"
     CDS             13844..14683
                     /locus_tag="JA154_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT7996313.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            14927..15023
                     /locus_tag="JA154_10605"
     tRNA            14927..15023
                     /locus_tag="JA154_10605"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14961..14963,aa:Sec,seq:tca)
     gene            15178..15885
                     /locus_tag="JA154_10610"
     CDS             15178..15885
                     /locus_tag="JA154_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HAT7996314.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(16027..>16365)
                     /locus_tag="JA154_10615"
     CDS             complement(16027..>16365)
                     /locus_tag="JA154_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT7996315.1"
                     /translation="FNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSS
                     LEFIKKISPDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEII
                     FTKYNYEIDT"
BASE COUNT         6509 a         1724 c         2758 g         5374 t
ORIGIN      
        1 tccaaatcta ttgcatatag ttattgataa tagaaatgta gagaggatat tatcacaaat
       61 aataataaat gtagccttat attatttata taattacata gtatctaaga atattaagaa
      121 aacagcactg gaattgcatg atacgaatga agaattaaca aaaaaaacgg aaagtttaaa
      181 agaaaagaat aaaaaattaa tctatgagac agaaaagata gaggaactta agggaatact
      241 tatgaaaaga gaatctaggc tacaatctac attagatgta gcagtaaata gcatagtagt
      301 atttagcaaa gatggagaaa ttacatatgc aaataagagc tttagaaata cttttggaga
      361 ctataaagaa caagataaat tgactgataa aataaaaaac ttcaatgatt tgatagaaag
      421 cattcattat gtgtttgttc atgagaagaa tgcagagaaa cttattagta caagcgataa
      481 taaagtttat caagctatat ttgcaccttt aattattgct tcacaaacag aaggagtatt
      541 gtgtgtacta gaagataaga ctaagaaaaa ggagtacgaa cgcaaactta ttgaagcaaa
      601 taagagaagt aaagattttt tagaaagtat tggagatggc atagtagtgc ttgaaggtaa
      661 taacaaaata tatgttaata atgcatgtag ggaaattttt aaaaataatt tagagagtat
      721 tgatttttca ttagtatgta aaagtgaaga aagtatagaa aaaagatatg taatagatgg
      781 tgaagtaaag tatgtagaga tgagtttttc tcagtatact aatgctggta ctaataaaac
      841 tattattgta atacgtgata caacctctcg aaaaatatct caaataaaac ttgaggaaag
      901 tcaatcatca tattcaagat ttatagatat attacctgat ggcatatgct tattaaaaga
      961 agatttaagt ataaactatg caaataagtc gtttttagat atgctagctt ttacaaacat
     1021 agatgatata aaagattcta atattaaact attaatgaat gcgaatactg aagaaaaaat
     1081 gaaatttacg gataatatgg ctaaagtttt aaatgaaaat agaagtatac ttttactgga
     1141 atatgaattg ataaatagtt atgatgatat agtagaagta gaattaagtg cattaccttt
     1201 tgctatatac aatactagat acataatgct tattataaaa gatttagtac ataagaaata
     1261 ttccgagcaa gcagaaaaag aactgcttga aagatttaaa acagataaaa tcaaaacgga
     1321 gttttttgca aatatgtctc atgaactaaa aactccacta aatgttatat ctagttcaaa
     1381 tcaactggta gattctttct atagaaatga aaagataaaa gattacaata acaatattaa
     1441 atctcatgtg gatttagtta gacaaagttc ttataggctt caaagattaa taaataacat
     1501 tatagattta acaaagatgg agtctgggtt ctatacacta aaacttgcga aatacaatat
     1561 tgtatctgta atagaagact tatttatgaa tatagaagag tatgcactta gaaaagacat
     1621 aaaaattctt tttgatacag accttgaaga aattaatgtt tatatagaca aggttgaaat
     1681 tgagagaatt atgttgaatc tattgtcaaa ctgtattaag tttactgata atggaggctg
     1741 gatatatgta agtattcatt ataaaataga taaagtaatc ataagtgtaa aagacactgg
     1801 agttggaata ccacaagata aattagagtt gatttttgaa gaattttcac aagttgataa
     1861 gactttatcc agaaatactg aaggaagtgg aatagggctt gctatagtta agaatctagt
     1921 aagtcttcat ggtggagata tagaagtagt aagtgaagtg aataaaggca cagagttctt
     1981 aatttcactt ccaatgaagg gatttagtag tgaacattac acagaagata agagaatata
     2041 taatattcaa gaaaaaataa aaatagaatt ttctgatata tattattaaa aatgattatg
     2101 agcaaaaata aaataaaaat actatttgtt taaatattta agttgtaaga aataggctat
     2161 cataaggtaa attattatga tagcctattt ttagctaggt tttatatcta tatgtgagaa
     2221 catataatct ctaaattacc ttcaaaacta aaatctccaa agtcgtttgg tagtataaaa
     2281 tgcataccct tttttaaatt gtattctaca gaattgtgta taagttttcc atttccatcc
     2341 aacacactgt ataaagaaaa aggtgtattg tgtgtaaaat tacattttcc aaaaacatct
     2401 aatttataaa cactaaaaaa ttcgtttgac acaaatgttg tgcatttaaa gtctgatgtt
     2461 gatacgattt tatagtcagt atcgaaattt atatgaggaa cattcgtaac atctattgat
     2521 ttttgaacat gaagttcacg cttattacct gaattgtcag ttctatcata atcatagact
     2581 ctataagtag tatctgagtt ttgttgagtt tctaaaatca atgttccttt gcatatagca
     2641 tgtatagtgc cacttggaac atagaaaaag tctccctttt ttatattaac ctttctaagt
     2701 agtttgtccc attcattatt atttaccata tttacaaatt ctttatgaga ttttgcatta
     2761 tgacctatta taatttctgc atcatcagaa caatctatta cataccaaca ctctgtctta
     2821 cctaactctc cattttcatt ttttttagca tattcatcat ttggatgaac ttgaactgat
     2881 aaattatcat ttgcatccaa tatttttgta agtaaaggaa acttatcacc aggtgtgttt
     2941 ccaaataatt ctctattttt attccacaat tcagatagtt ttttaccttt atattttcca
     3001 ttttctatta agcaatctcc atttttatga gagcttatag cccaacattc accagttgtt
     3061 ggcgaatcta tttcataatt aaatttatct ttaagtgcag taccacccca tattctatcc
     3121 ataaaaatag gttttaaaaa taatggttcc ataatcctgt tcctcctatg atattaaatt
     3181 tatcctacaa ataattatat ataaatagat tataaaatca atagtaaaaa agttttgtcc
     3241 cttgaaatga cataaagtat aaggagttat taaataagat aaactatgat atataatata
     3301 aagttctaaa ttggagtgat aattatggag ttgtttaagg cagaaagcaa aaaatataaa
     3361 aaagaatttt taaaattatt ggagcaatgg gttagtatac cttcatttta tgatagaaaa
     3421 actgtatcta aagacatgcc ttttggaaaa ggagtatatg atgcattaaa ctggtttgaa
     3481 aatctaggta gagaaaacaa tttcaaagta aaaaatatag ataatcatgc tgttcaaatt
     3541 gaatatggaa atggtaaaga gtatgtagat atatttggac attgtgatgt tgtaaatcca
     3601 ggtgaaggat gggatagtga accatttaaa ctaaatatta taggtgataa attagttgct
     3661 agaggtgtat ctgataataa agggcctatg atagtgaatt ttttagcact aaaaatgata
     3721 aaggatttag atattaattt aaaaagaaag gttagattaa tagctggggg aaatgaagag
     3781 agtggattta aatgtataaa gcattattat agcaaagagc catatggtgt atgtggattt
     3841 actccagatg ctaaatttcc tgtattaaat ggagaaaaag gtggagcaat aataaagtta
     3901 atttcaaata tagacgataa atctttatat ataagtggtg gaatagagtt taatacaata
     3961 ccagataaag tttatataaa gaatgtagaa aaattgggta aagataatat atgttttgat
     4021 attaacaata tatcgataaa ttatgataat ggcaattata ttgtgtatgg aaaaggtggt
     4081 cattcatcta aacccgaaaa atcaataaat cctatattag ctacaattaa acttttgtca
     4141 gaaaatattg atgaaaaatg gacaaaagat ttatataagt tgataaatca agataatata
     4201 aatggaaact tatttggttt aaatattgag gggaaatgtg gaattttatc aatggtgcca
     4261 acaataataa atatagtaga tggaaaatta gaagtagttt taagcgttag atatccagaa
     4321 atattaacca ttgaagatat aataaaaaaa tttaatttgt atatggagca aaataatatt
     4381 aataaatttg agcttattgg ggaaaattta aagcaagcaa attatataga tagaaattca
     4441 aaacttgtaa gaagtttgca tgatatatat ataaagtatt ctggagattt aaaaaatgat
     4501 gttagagtaa ctagtgcagg aagttatgct tctgaaatga ataattcagt aatatttggt
     4561 tgtgaatttc ctgatggtag ctttggcaat gtccatagtg cgaatgaatt tgctagctta
     4621 gatagattta taactgcaat aggtatatat gcagaggcaa taattacatt atgtaataaa
     4681 atataaatac aaaatgtccc ttatatagac atattatata tacaattaaa attttacttg
     4741 atatataatt gaaatcaaag agaaggtgat acaatgataa ctaagaaaca tgcaataata
     4801 gtaaagagtt taagcaataa agatggatat atgacatcaa acgaattagc tatcaaattg
     4861 gatgtgtcta ctaaaactat taaaagatat atagcagatt taaatagtgt tttaagcaaa
     4921 tatgatttgg taatagcttc ttctagaggt ataggataca agttaagcgg ttctaaaaac
     4981 aatatagcca gagctgttaa agaagcaaat aaatatattg atggtttttt agatgactct
     5041 gaagagtcaa gaatgtcaaa tataatatgt atgcttatta atagaaatta tatgagtata
     5101 gaagcaatgg cagaagaatt aaatctaagt atagccgcta taaataaatt gtctagtaag
     5161 cttaaaagaa aacttgaaaa gtatgactta gttataaaat ctaaacctta ctatggaagc
     5221 tatatacatg gtgaagaaat taatataagg caattaatta ctgactatgc aataaaatta
     5281 gatgaaaaaa gtaaagtgaa ggtgttttta gatgacatta gtgaaaacga tatacactgt
     5341 atagaaagta ttttagaaaa gcacttaaga gaaaaagata caataatttc agataaagat
     5401 tttaatcttt tgctttcaaa aataatcgtt tctgtattta ggagtaagag aggtcatagt
     5461 aacaatataa acttaatgga tacaagttat agatttcata actactactt tatagaaaat
     5521 ctaatgaaag aaatatctga taaaattgga tttaaactta ttgaagatga agtaatttat
     5581 atttcaaatt attctggtgt aatagcctat aaaggtacac aaggaagaaa aaacacaagt
     5641 gaaattgagg aacgaataag tactgttata agtagtgcac ttcaagatat attccttatt
     5701 tcaggtagtg attatacaaa agatgatgag tttatggttg caatctctga ccatataaag
     5761 agatttttaa atagagctag agctaatgta aaatcaaata atcctttact tcatcaaata
     5821 aaagagaagt ttcctatagc ttttaattta gccgtgtttt tatcaaataa actagaaact
     5881 gagtttaatt taaaacttga tgaagatgag cttggatata tagctatcca ctttgctgct
     5941 tcaaatgaaa gaatgaaaaa aaatactagt aagaaaattt gtatagtttg tcattatggg
     6001 ataggaacag gacaacttat ttctgaaaag ctaaaacaga atataagtga tttaagtgtt
     6061 gtgggtgttt atccagttag atatctcgat atggctataa gtcaagatgt tgatttgata
     6121 gtatctacag ttgaattaaa aggatatgaa aaacctgtct tatatataga aaatatattt
     6181 gatgatagct taattgaaaa cgtaaacaaa gccttttatg aaaaggaaga aagaagaaag
     6241 ataataagta acatgtttga tgaaaaagct tttttcagta taaaggctag tactaaagaa
     6301 gaggtcataa tttcactttc aaataaacta aaagaaagag attttataga ggaaagttct
     6361 ataaaaagta taatagatag agaaaatatt tcatcaactg aaataggaaa tctagtagca
     6421 ataccccaca caatagtgaa gggagataaa aaatctataa taggagttgg aatacttgaa
     6481 aatccaataa tttgggataa gcaagaggtt cagctagtat ttatggtatt ttttaatact
     6541 aaagaaaaac ataatttctc gatatttaaa tacctttata attttataaa agatgaagga
     6601 ggagtaagag gaattattaa aatatgtgac tttaataaat taatggcact tataggtaat
     6661 taaggaggaa attaataatg ggaacaaaca tatttaacaa agaatatgta tttttaaatg
     6721 ttgatgctaa gtcaaaggtt gaagttttaa aatttatatc aaaaaaagct aaagacttaa
     6781 acttggcaga agatgagagc ttagtatatg aaggtttaat ggctagagaa gaacaattta
     6841 ctacaaattt aggagaatct atagcaatac ctcacactaa aaatgatgca attgaaaatc
     6901 cagcagtagt agtattgaag tttaatgaag atgttgtttg gaatgaaggt gaggataagg
     6961 ttaaattagc aataagctta cttatgccag gaaaaagtaa agagaatatt catcttaaat
     7021 tattatcatc tctttcaaga aaattaataa ataaagaatt taaagattct cttttaaaaa
     7081 gtgataatgt tgaagaaata tctaacttaa taaatgaagc tttaggttta taattaaaca
     7141 ctaaggttat aaaataaaaa aataattaac taaaaatagg aggaatttga aatgaataaa
     7201 aaattagtag cattatgtgc ttgtccaatg ggactagctc acactttcat ggcagcagaa
     7261 gcaatagagc aagcagcaaa ggcattaggt tatgaagcaa aagttgaaac tcaaggtgct
     7321 gatggtgtac aaaatgagtt aacaagagat gatatattag gagcaactat gataatacat
     7381 gcagtagcaa taacaccaga aggtatggaa agatttgatg gttgtgaagt ttatgaagtt
     7441 gaattacaag aagctataaa aaatgcagaa ggtgttataa aagaaataga agaagattta
     7501 ggaatataat aaatttattg tggaggaaat agaatggcaa ttaagaaaaa agttataaac
     7561 agttcaggtg cttctacaac aggaggaaat aatgtaggaa aatctactcc atctagtcca
     7621 actcaatcaa agggaaatgg aaaatggaaa gaaataagta aacatataat gacaggtata
     7681 tcatatatga taccagtttt agtaatgggt ggacttatag gggccttatc tcaattaata
     7741 ccatatgcta tattaggact tgacccatct gtaggtatag ttgatgctat gaactcagga
     7801 gaatttacag ggttcaagct tagtctttta aatatagcac aattaatgtc aaatttcgga
     7861 tttactttgt ttggatttgc gataccactg tttgcagcat tttgtgcaaa ctctataggt
     7921 ggaaaaacag ctttaattgc aggatttata ggtggatata tagctaataa acctgtaggt
     7981 gtaccacaat tcgttgatgg acaatggact gaagttgttc cagttgcatc aggattttta
     8041 ggtgcaatac taatagcatt tataatagga tattttgtaa aatggttaaa taaatctata
     8101 aaagtttctc ataactggtt agcttttaaa acaacatttt taataccatt aatagcgtca
     8161 ttagcttgta tggtattgat gatatttata ataactccat ttggtggatt aattaatgaa
     8221 agtatgaaaa acttcttaac agcagcagga gcagctggag aatatgtata tgcaacagct
     8281 ttggcagcag ctacagcatt cgacttagga ggaccaataa ataaagcagc aggattcgtt
     8341 gcacttggtt taactacaga gaatgtactt ccaataacag caagaacaat agctatagtt
     8401 gttcctccaa ttggattagg attaacaaca ttattagata aaagattagt aggaagaaga
     8461 gtatatgata gacaattcta tcaagcagga aaaacttcta tattcttatc atttatgggt
     8521 atatctgaag gagcaatacc atttgcactt gagagaccag gttttgttat acctttaaac
     8581 atagttggaa gtgtaatagg agctattaca ggtataattc taggagcaat tcaatggttc
     8641 ccagagtcag ctatatgggc atggcctcta gtagataact tatttggata tataataggt
     8701 atagcagttg gtgctatatt catagcagta ggaaatatat tctatagaaa taaattaata
     8761 aaagatggta aacttgttgt agattatatc gactagtatt taatttttac tatctataaa
     8821 taactttggc tggtaatatg gttttaatca atattaccag ccttacctat atgattaatt
     8881 aaaggcggtg gagataagat atggataaga tagaaaaagt aagagagtat tttagagaat
     8941 atgatataga tggatttctt ataaatagtc ctacaaacaa gttttatgtt ggaaatttat
     9001 ttagctcttc aggttatgtt tttataacta aagaatcaca atatataata gtagatttta
     9061 gatattttga agaaattaaa agaaaatcta gtttgtttaa tgtagtatta atggacaaaa
     9121 ctagaactca ttttgatata attaatgata tttgtagaga gcagaatata aaggaaattg
     9181 gatttgaggg aaatgaagta tcttttgatt tatatagaag tatgagtaat aagttaagtg
     9241 caaccttaaa atcagttgat ttatctacat taagggaaac aaaaaatgaa gacgaaataa
     9301 aatatataaa aaaagcttgt gaaatagttg atgctacatt ttatcatata gtagacttta
     9361 taaaggttgg tatgactgaa aagcaagttg aaaatgaaat tgttagaatt ataaaagatt
     9421 taggtggtca aaaagaatct ttcgatacaa tagttgcatc tgggcttaga ggagcattac
     9481 ctcatggaaa agcaagtgaa aaagttatag agtatggaga ttttgttacc tttgactttg
     9541 gtgctaagta taataattat tgttcagata ttaccagaac catttgtatg ggtactataa
     9601 ataaagagtt agaagaaata tataatatag taagaaaagc taatgaagag tgtataaggg
     9661 ttttaagacc gggaatgact actggagaga tagataaagt tgcaagagac ataattggtt
     9721 catatggata tgccaataat tttggtcata atctgggtca tggtgtagga attatggttc
     9781 atgaatatcc tgcattagct cctgaatcaa atgaagtatt aaaagaagga atgattgtaa
     9841 ctatagagcc tggtatatat gttccaagtc ttggaggcgt aagaatagaa gatgatgtac
     9901 ttataacaca agatggatgt atgagattga ctacatctac taaggattta atagttgtaa
     9961 gctaaaaata aattagataa atattttata acaataagaa aagaagaggt aaatttaaat
    10021 gtataatttt gatgataaac cagatagagt aagtgaaaag tgtagaaaat gggatttaaa
    10081 tattataaga gataaatttg gagatattag agaagatttt ataccaatgt ggattgctga
    10141 tatggatttt aaaataccaa cagaaataga aaacaaattt attgaagctg taagaagagg
    10201 tgtatttgga tatacatact gttatgatga attttatgat tcagttattg gttggcaaaa
    10261 aaatatgcat gaggtaaatg tagaaaaaga atggattaca ttaacttatg gaactgtatc
    10321 aacacttcat tatgtagtac aagctttttg taagaaaggt gatagtataa tattaaatac
    10381 accagtttat gacccctttg aatcatcagc aaaaaaacaa ggtgtaaatg taatttgtaa
    10441 tacattagat gttgtaaatg atagatatta tataaatttt gataaattag aagttcaaat
    10501 taaagaaaat aagcctaaat taatgatgtt ttgtacacca cataatccat ctggaaggat
    10561 atggacaatt gaagaaatga caagggtagc aactatatgt aaagaaaata atgttatttt
    10621 ggttgcagat gaagtacatg cagagcatat tcattatggt acatttaatt caatacttaa
    10681 gattggaaaa gagcttttag aaaatgttat tctattgaca tcaccaaata aaggatttaa
    10741 tcttggtgga ttaaagactt cttattcaat agttataaat aaggatataa gggacaaatt
    10801 tagaaataaa ttaaagcaaa attctataac ttctccaaat gtgtttggaa taataggatt
    10861 gataaccgct tataatgaat gtcatgagtg gttaagtggt gtaaatgaat atataaaatc
    10921 aaattatgaa cttttagaaa cttgggtaaa taaatataat aagataaaat tgatgaaaat
    10981 ggaatcatct tacttagctt ggatggatat aagtggatta ggtattagtg caagtgagtt
    11041 tacagataaa ttggctatag atacaggagt attattagaa gatggaagtc attttgtaaa
    11101 agatggtgag aagtatgtta gaataaattt aggaactcaa aaggaaaatg taattgaagc
    11161 cttaaataga atggatttat ttttaaagtc tttataaaaa tttggttgaa ttagtatttt
    11221 atataaattt aaactattaa tatggtatat acataagtag aatttgtttt agattactta
    11281 agtatatacc atattttata taaaaattaa atttgcatta gtcatgtgat aactatttaa
    11341 attcatatgt attttgtatg tataatagat ataattatta tttaaactta ttgtttattt
    11401 aatttttcaa tagaaaaaga tttatctgta agtttaaaat tatcttttat acccttagtt
    11461 aagtatactt tcttatcctt agttatgaat atagcatcta cattatcaat tttattaatt
    11521 aaattcatac ctttttcaag accaagacca aatgtagaag tggataaggc atcacaatta
    11581 atagatttat cagagattat tgtaacacta cttaaattgt tttcaaaagg ataccctgta
    11641 aatggattta acatatgatg atatattttt ccgtcttttt cgataaatct ttcataaatt
    11701 ccagaagtaa caacagactt atttgtagtt tctatgtttc ctatggattc accattttta
    11761 ctagttgggt cttgaatacc tattttaaaa ggtttatcat ttttaccatt ttctaaagta
    11821 aatatatttc ctcctaagtt tactaaacct ttttttacat cctctgattt taaataggct
    11881 acaattttat cagcagcata tcctttagct atggcaccta aatcaatttt catattttct
    11941 ttggttaatt ttatagattt atttttttca tccaatatga tgttcttata atcaataaga
    12001 ggcagaagtt tttgtatctt agaatcactt ggaactttag ctttatctgt tccaatagcc
    12061 cataaatttg ataatggacc aattgttatg tcaaaatatc catctgataa tttggaatat
    12121 tgtatagctt cttttacgac gaaaaatgta tcatcagaaa ctctgacaaa ttttttacca
    12181 gcattatcat ttattttaga aacatcactt cctggtatat gagtgctcat tttgttgtca
    12241 atatatctta aaattgaatc acattcattt aagattttat cacttttgga ttcttttaca
    12301 ttaaatacag taacttcgtt tactgtacca aggtagtagc tagttttact atatttagta
    12361 ttctggcttg aactcctaaa aaatgaaaat aaaattaatg ctaaaatcaa tatcacaaaa
    12421 ataaatgtga tttttttctt attcatggta tattcctcct atgaaattgt gttattattt
    12481 tattataaca taatttcata ggaggacaaa aaatgaagaa aaaagacatt cttcttattt
    12541 taggcatttt ggttgttatt acagcatgtt atgggataat aaatgttata aattctaaaa
    12601 atgctggaaa tattgagatt tatgtagata ataaattgta taaaactgtt tcaataaatg
    12661 ccaaagaaga atttaaaata gagagtagag gggggtataa catagtaaag attcatgata
    12721 aaggggttga gatagtagat gcttcatgcc ctgataaggt ttgcgtacat actggattta
    12781 taaacaagcc aagtcaaagc atagtttgta tacctaatag agtgagcata aaaataaata
    12841 caaatgaaaa gaatgataat caagaagata taatttctaa ataaaataat aaaaataatt
    12901 gaaaggaatt ggtgataatt atggttaact taggaaacga ttgggatgaa ttattgaaag
    12961 aagaatttga aaaagattac tacttaaact taagaaaatt tcttatagaa gaatataaaa
    13021 cacgccaaat tttcccaaat atgcacaata tatatgaagc tttaaaacac acttcataca
    13081 aagacacaaa agtcttaata ttaggacaag acccttatca tggagataat caagctcatg
    13141 gtttagcttt ttcagttcaa ccacaagtga aaactccacc atctttatta aatatgtata
    13201 aagaattaaa agatgattta ggttgtttta tacctaataa tggatattta atgccttggg
    13261 cagaccaagg agtgttactt ttaaatactg ctcttactgt tagagcgcat gaagctaatt
    13321 ctcataagaa caaaggatgg gaaattttca ctgatagagt aatatctatt ttaagtgagc
    13381 gtgaagaccc agttattttt gtactatggg gaagtaatgc acgtaaaaaa gtagagttga
    13441 ttgatacaag taaacattat attttggaag cacctcatcc aagcccattg tcagcaagta
    13501 aaggcttttt tggatgtaaa cacttctcta agataaatga aattttgaaa aagctaggaa
    13561 aagaacctat taattggcag atagaaaata tataaaagta aaaggtaaca aaatgataac
    13621 aaaacaacaa taaacaatga cattgttgta actaatagaa atatgcttaa atagttgaaa
    13681 atactatgta tgcatatttc tatttttttg tcaagttgac aactcttgaa aaaatagtta
    13741 caatttagtt acacaaaaaa cacaaataaa aaaagaaact tacaaagcag aattttatga
    13801 agcaaaatat atagtgtaaa aaatcttagg gaggaaattt tatatgaatt ttaaaaaatt
    13861 gagtaatgct aaagtctcgg taatggcagt tgttttatca gttggaatat tatcgggata
    13921 ctctgcgctt aataaacaag taacattagt agtaagggga gaagaaagaa ctatatcaac
    13981 atttagttct aatgtaggag accttttaga atctcaaaac attagctatg attcaaatga
    14041 tatagtaact ttagatgaaa atacaaagtt atctaatgga gataaaatag aagtaattga
    14101 tgtaaaggaa aaaacagttg tagaaactaa agacattcca tttgaagtta cagttgttga
    14161 agatggctct ttattaaagg gtgattctaa ggttaaagaa gaaggaaagt ctggtaaaag
    14221 tgaattaata tataaagaaa cttatcacaa tggaaagaaa gtagaaaagg aatttgtgaa
    14281 aaaggttgtt aaagctgagc cagtaaataa ggttgtaagc aaagggacaa aggtagaaat
    14341 gcaagtagcc tcatcaaggg gagaaagttc aagaagaatg gcttcatcat catctagtag
    14401 cagtagtagt aacaatggta aaaatatgag agtggtagca actgcatata ctggtcacag
    14461 tataactgca acaggaacaa aacctaagtg gggaacaata gctgtagacc ctaaggttat
    14521 accatatgga actaaagttt atatacctca atttgacatg gtgtttactg cagaagactg
    14581 tggtggagca ataaaaggaa ataaaattga tatctacatg aatgattcaa aaacagttta
    14641 taactgggga agaaaaacta tagatatacg tatattaaaa tagttaaata atttatattt
    14701 ttactggaaa ttatattttg attatataga gaagaaactc tttatagatt ctaaaaatat
    14761 aatttccagt tttttatttt ggatatgtaa taaaacaggt aaattttaac catgtataga
    14821 taaaagctat ttaaattata atttactatc atataaatct ataaaaattg ttgcaaagag
    14881 tcaaaatata tgatattata gatttcgttg agaaggattt cattatggga gtggatgggt
    14941 gccggtgtgc cctctagtct tcaaaactag tatgaggggt taagagcctc ttaggtgggt
    15001 tcgattccca cgcattcccg ccaatatgta aaaggtatct tttaattaaa agataccttt
    15061 ttaaatttat tttaagataa tttattttta ataatatata atgttttgta ttatataatg
    15121 taaattttct aatacaaaac attatatatt tagataaaaa tctaggagtg tggaaagatg
    15181 gaaaataatt ttacaacgag aactagtttt ctagttggag atgatgggat aaaaaaactt
    15241 aataattcaa atataatagt atttggagtt ggaggagttg gtagttttac tgttgaagct
    15301 ttagcaagag ctggagtagg caatattact attgtagatt ttgatgatgt agatataacc
    15361 aatataaata gacaaatacc agccctacat tcaactgtag gaagatacaa agttgatgtc
    15421 atggaagaaa gaatattaga tataaatcct aatatcaata taaaaaaaat aagaagtctt
    15481 tataataaag atactagtga cgaaatacta actgaaagat atgattatgt tgtggatgcg
    15541 atagacatgg taagttccaa aatacatcta attgaaactt gtgaaaaaaa aggattgaaa
    15601 ataataagtt ctatgggtat gggaaataaa ttagacccaa ctaaaatagt cgttacagat
    15661 atacacaaaa caagtacttg tcctctagct aaagttatga ggaaagaact tagggataga
    15721 ggtatcaaaa agttaaaagt agtatattct actgaacaac caatagaact taagaagaaa
    15781 gtaatgaatg gtagaaaagt aacacctggt agtgtatcat ttgtcccatc tgtaggaggt
    15841 ttaataatcg catctgtaat agtaaatgaa ctattaggac aataagtttt aaatatttta
    15901 atttataaat tttatataca aatttgaagg ctagaagcat gaatacaaag caattttata
    15961 ttcatacttt tagcctaatt attttatatt accttatttt atgtagttgt agttttattt
    16021 tttagattat gtatctattt cataattata ttttgtaaat ataatttctc catcactata
    16081 aaaaacaata tctcctattc tatctgttct ataaattgat acatggtttt gttttagatt
    16141 attaataacc tccctatgtg gatgaccata ttgattttta tatccacatg atattgcagc
    16201 tatatctgga ctaatttttt ttataaattc taaagatgaa gaagaattac ttccatgatg
    16261 acctattttt ataaaattaa tattatttaa tttaaaagaa tgtaaaattt ctttttcatt
    16321 ttcttcttca gcatcaccca taaataaaaa agaattatca ttaaa
//
LOCUS       DACULQ010000083        16364 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.083, whole genome shotgun sequence.
ACCESSION   DACULQ010000083 DACULQ010000000
VERSION     DACULQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.083"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(236..457)
                     /locus_tag="JA154_10620"
     CDS             complement(236..457)
                     /locus_tag="JA154_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small acid-soluble spore protein"
                     /protein_id="HAT7996316.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(541..1767)
                     /locus_tag="JA154_10625"
     CDS             complement(541..1767)
                     /locus_tag="JA154_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HAT7996317.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(1829..2761)
                     /locus_tag="JA154_10630"
     CDS             complement(1829..2761)
                     /locus_tag="JA154_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996318.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(2850..3377)
                     /locus_tag="JA154_10635"
     CDS             complement(2850..3377)
                     /locus_tag="JA154_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAT7996319.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            3606..4694
                     /locus_tag="JA154_10640"
     CDS             3606..4694
                     /locus_tag="JA154_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAT7996320.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFILKTY"
     gene            complement(4857..6047)
                     /locus_tag="JA154_10645"
     CDS             complement(4857..6047)
                     /locus_tag="JA154_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT7996321.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGIDTVGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTPEQEGRLLAFMEKDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLKDSEMGYYIETPKTKNGKRELPLTE
                     RAYQAIQRILKNRGKAQPLIVGGYSNFLFLNREGLPKVAGNYEGMVRGLIKKYNKYHT
                     DKLPNITPHSFRHTYCTNMANRGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(6125..6328)
                     /locus_tag="JA154_10650"
     CDS             complement(6125..6328)
                     /locus_tag="JA154_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAT7996322.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENLDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            6438..6668
                     /locus_tag="JA154_10655"
                     /pseudo
     CDS             6438..6668
                     /locus_tag="JA154_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274596.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6642..6875)
                     /locus_tag="JA154_10660"
     CDS             complement(6642..6875)
                     /locus_tag="JA154_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT7996323.1"
                     /translation="MPKELTPTFTVISKACDGDETAISKILEFYDPYINRCCMRPFYD
                     DNNNLRLAVDLEMKGFIKEAIIRKILNFDIKRF"
     gene            complement(6860..7300)
                     /locus_tag="JA154_10665"
     CDS             complement(6860..7300)
                     /locus_tag="JA154_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996324.1"
                     /translation="MKPSELQEKTCHQFDCAIKTVIRHRAIDFYRAIRRSEKKEMPFA
                     EIMDDLLNSIGTEDTYSTDYVAVFEVCGEKVSIEDEQLARALKAIEERKRNIVLQYFF
                     IGDTDTYIGKMLDCSRANVTKCRIQALDKLKQLIEEEKEKCQKS"
     gene            complement(7575..8210)
                     /locus_tag="JA154_10670"
     CDS             complement(7575..8210)
                     /locus_tag="JA154_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996325.1"
                     /translation="MLEVKNVSYAYKTKKDKTILNNVSATFEEGIFYTIVGPSGAGKT
                     TLLSLLAGLDTAVKGTVLCNGEDITKKGLNYHRKHNISLVFQNYNLIDYLTTVENVKL
                     GGSGNAEKLLEEVGIGKEYWQRNVLQLSGGQQQRVAIARALASDAHVLLADEPTGNLD
                     ETTADEIIALLKKTAHELGKCVVVVTHSKHLAEEADEILEIKNGAISFLSD"
     gene            complement(8224..9669)
                     /locus_tag="JA154_10675"
     CDS             complement(8224..9669)
                     /locus_tag="JA154_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996326.1"
                     /translation="MNFFGLARRSVFRKPIKSILLLLIVFAISTLLLAGVASKNASIE
                     VQDKTRQAIGAGFLLERNAEYRTKRVREYSEKIGNDKEGSFGGYHQEKEIINGVETWS
                     GWTTNEFESLDIKDIEKLAKTKGIADYNITTATTPVNPVNFKRIEDKDVDQSVDEGGV
                     SLIGNKDMKLDRNVLSGNLHIKEGRMITPEDKDMCVISEELAKQNNLKIGDKISFNDY
                     HDTVNSKVSEAEIVGIYQVDQKMSPLMQGDTYRSENVIFTDLRFPEKAEGETSPLYER
                     AYFKVEDVEAYDEVKENLQKVDINWEQYDLIDNNGNSETMSSNFNDLAKVSEMMILVI
                     SVASFVILVLVFLFWLKNRVQEVGIFLSLGVPKFRIIGQIWSEAIMIAVLSLMLSFAV
                     APAVSKVTANYLVSQQVQQMQEEEKNNEGKVSTEYVAPKQDVQNISVEVTPEMYLLDG
                     VSVLVLITASVLVSGIVILKRNPKDILSEMS"
     gene            complement(9673..10896)
                     /locus_tag="JA154_10680"
     CDS             complement(9673..10896)
                     /locus_tag="JA154_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996327.1"
                     /translation="MKFYQLGFRYLQRKKGKTILLLIVLILVNSMILGTSMILRTTNE
                     SKQAMQEKTNSKIVAEITSKDSKITENEVNKIETLEDVSSINRIGRQEVFPNDFAPVT
                     LNTSTNEENLKIALLSYDDLEKDSPFSEMQYRLSSGDYIKHEKKGAVINANLANQNEL
                     KVGDTIELSTENGTKVSVQIIGIFMSAGNVEKDQPTETTAVNRIENQVFIDNSTYKEL
                     FKEAEFEKLCVYSKRPEKLDVLETSLQKIFGNDVELSTSDTLYQQMSAPLEQISKVAN
                     LMLVLTLVTGTVVVSLLLCMWMRTRQKEVAIFMSLGKTKSEIFLQTLLEAGSVFTLSV
                     LGATAIGKLFSGVLQNVITGSETVTENLKVVLQIQDIGMLFLLGGAVILVALCCSILP
                     ILRANPKDILAKMEG"
     gene            complement(10908..11405)
                     /locus_tag="JA154_10685"
     CDS             complement(10908..11405)
                     /locus_tag="JA154_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HAT7996328.1"
                     /translation="MKKTNISKFVAVGLCICALTGCGASPDEKPDTSNPIVNSNTNEE
                     NANGSSENKGNDILESANLIGSVLEFTDNGCSVSQAKETEGGASLQIEASGLENKDNA
                     VSVTYNPDCEFFVATLNKQLGSVTNITTGSISDVKKQSNVYLYGEFSDTLHFNATKVV
                     IARWE"
     gene            11568..12254
                     /locus_tag="JA154_10690"
     CDS             11568..12254
                     /locus_tag="JA154_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996329.1"
                     /translation="MKILLLEDDLELCKNIKISLEKENYMVDCCNDGETAMLYALNTD
                     YAYDLAIVDRMLPIIDGLTIIKSMRRKGISIPILIITAMSTLDNRVEGLDGGADDYLV
                     KPFHIKELLARVRALTRRPANIQLSSKLEYGDLTFDKDSRTLSTPVNSLLLTSKETEL
                     TFVFMQNPETLFSREQLILKIWGSSSEVETGNVDSYISFLRKRLKELKSSCKITTVYG
                     AGYKLEKETC"
     gene            12248..13540
                     /locus_tag="JA154_10695"
     CDS             12248..13540
                     /locus_tag="JA154_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996330.1"
                     /translation="MLNNLYRKLHILFASSVMLIITLVISFVVANTVHTEKINESTLF
                     QRLTTLLIYQVESASSDMDKALKTYEESYHIFSLITDTRGNTIYQSDFPFPTSADNLL
                     HDVEKQISTQPLSQIENSTTSQGGFLEIRGTHHDTYFVIPATIRTANDTVYHATFLYQ
                     TASLTDILQKTLPIYLLIWFLACIVVIVLTRYLLKKSFAPTERILQSQKDFVATASHE
                     LKSPLAVMISNTDMLLDNVSLNEQAKQAVQTIDFECMRLSRLVKDMLLLASSDAKTWT
                     LHKSTINVDTLLITLYETYEPVCMKQNLELKLHLSEESYPAMFTDKDRLFQILCVFMD
                     NAIQHSKNNSLIEIEVIATEKNIAFSVVDHGQGISDEDKQYIFDRFYSGDKSHTNKSN
                     FGLGLSIAKELTQMLNGIIIINDTAGGGATFTVKFPLK"
     gene            complement(13586..13810)
                     /locus_tag="JA154_10700"
     CDS             complement(13586..13810)
                     /locus_tag="JA154_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996331.1"
                     /translation="MIGLKKRDIKKALKAGAKAGGVSLADVRADIEATIDEAMNSTAP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTKI"
     gene            complement(13856..14551)
                     /locus_tag="JA154_10705"
     CDS             complement(13856..14551)
                     /locus_tag="JA154_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7996332.1"
                     /translation="MCTRFVYNGIDKIVGFNFDIDLSVWDHTVITEENRFYIGIKMPD
                     GLYHSFHGINKNGNVGTLLYVHGNENARYIESEDCITISELTEKFIKAEISFDKALDI
                     VKKKKIIYAPDATMQAMLSDKKGRVLIIEPGIGYRYEQENFSLITNYSILKPDITKPY
                     IVSGDTRYEVAKELLAGYDKDFSVNDAFKVLKSVSQKDLWATRVSFVYSTEKNKVYYV
                     LNNDFSNVFEFQF"
     gene            14729..15286
                     /locus_tag="JA154_10710"
     CDS             14729..15286
                     /locus_tag="JA154_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7996333.1"
                     /translation="MKTTKEAVIKATSDLADEKGLNNVSLKAVAQKLNIRTPSLYNHI
                     ESLDNLLLEVAHNGMRDMNERMMKIAVGKIGKEAIKLVSIEYLNYMIEHPGVYETIQW
                     AVWHGTEETATIFNNYLSLLTTLIQSCSLNKDKTLEILNMLTGIIHGYTTLQLGNAFS
                     ASDKVRFELAEAIDTLLVGIFQKYK"
     gene            complement(15333..16244)
                     /locus_tag="JA154_10715"
     CDS             complement(15333..16244)
                     /locus_tag="JA154_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7996334.1"
                     /translation="MFKKNKKQTETVKEPKEKKVRTVKVGTHKKTMIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIEARTQA
                     ISGYLTKELQDLNVDTIRTDIPTSSTVTDVIVWHIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTVNDATA
                     FLETFFKLYPTATEKELAYYVSGNVIEPIGRDYLYSELVNPIFTKNGDNVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDSNWKIVG"
     gene            complement(16260..>16364)
                     /locus_tag="JA154_10720"
     CDS             complement(16260..>16364)
                     /locus_tag="JA154_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAT7996335.1"
                     /translation="GNNQMYHAGDPIGYADLSSSYWQQHLIGAGRVKQ"
BASE COUNT         4911 a         3203 c         2410 g         5840 t
ORIGIN      
        1 ctattaataa atttgttttt taaatcctca aaatttacac tactcatttt acatcactcc
       61 ttatgtaaca tattcatttg tattatttta tccaaataca taaaatttat tacataaaaa
      121 tatatctatt taataattta ataaaaaaaa gctatctcaa tttagaaatt actttctata
      181 tttgagatag ctctagctaa actaatagcc aatagttata aataattatt tataactatc
      241 tgttgctttt tccagccatt tgttgttcag ccatttcaac taatttttta gtcatgtatc
      301 cacctacata accattttct cttgcagtaa ggtttccttt atcaacttgt tgataattac
      361 tcataccaag ttcattagct atttctaatt tcatttggtt taaagcctct tttgctcctg
      421 gtactactgt tctgttgttg ttattgttac ttgccatgtt gattaccttc cttctgttta
      481 atttattttg tgttgctgat aatattatgt gattttaaac atttttaatt caggtaattt
      541 ttagcaatta aaacctgcca aatatccagt agaaaaagct atttgcaaat tgaatccacc
      601 tgtatatgca tctatatcta ttacttctcc agcaaaaaat aaaccttcta ctaattttga
      661 ttccattgta cttgaattta tttcgtttac ttttacacca ccagaagtta ttatagcttc
      721 ttcaataggt ctgtatctct ttactgtaaa ctttaaattt tttaataaat ggactaagtt
      781 ttttctctgc tctctagaaa tttggtgaac aaccgtattt ggattaattt cacttaagtt
      841 tattattata ggtattagtt tttttggaag taaatcatct aatgcttttt caaatttttt
      901 atttgtatac ttctgaaaat ctttttgaac tcttttatcc aatttttcct catctaatgc
      961 aggttttaaa tctaataata tagtgtaatt ttctttcctt gtatctttca ttctacaact
     1021 tgcagacttg attataggtc catctaaacc aaatttagta aattcaagtt ctccaaaatc
     1081 actataaact ttcttttgtt tgctattaaa cacccttatt tcaacatttc tcaatgataa
     1141 tttttctaaa tcttttgtaa aactttcttg tacttctata ccaattaaag atggttttgt
     1201 atctataatt gtgtgcccct gtgaaattgc aaatttatat ccatcaccag tagaaccagt
     1261 aagtggataa cttaatccac cagttgctac tacaactgaa tcacacttaa tttctttttt
     1321 atcgtttaaa attacttttt caattttatt atttttagat attatttttt ctactttcga
     1381 atttaataat atatttactt tcttactttt aagttgtctt tcaagagcat ttacaatatc
     1441 aaatgcttta tcactctctg gaaaaaccct ttttcctctt tctgttttag tctttactcc
     1501 aagattatta aacatactta ttacatcatc atttgtaaaa gtataaaaag cactataaag
     1561 aaattttcca tttgtaggta cattttctat taattcctct atttcacaag cgttagtaat
     1621 attacatcta ccttttcctg ttattgcaag tttttttcct aatttatgat ttttctcaat
     1681 taatgttaca tcatagcctt tctcacatgc agttgatgcg gctatcatcc ctgctggtcc
     1741 accacctatt attactacct tcttcaaata atcactcttt tctaccctta atttttttaa
     1801 tttaaactat tgactctata ccttaaaatc aatttaattt taaaagttta aataatgctg
     1861 gagctcctgc tattccataa aaaacaacta ctgtattctt aataaaattc ataaaaatta
     1921 tactcatagt agagtcactt atatttaaag tgactattaa cattgaaata aacaacttaa
     1981 tgccaatata taacattcct attgttacta tcccaaccaa aaacctacat attcttgaaa
     2041 aaattatatt tttactcccc ttatttttat tactaaaatt tatttttttg ttattattat
     2101 ctgtattaaa tttaacaaat ctatcctcaa ccatatatcc aaatacaaat cctactatta
     2161 taccaaattt ttcaaaatat tcagggctat ttagaaatat agccactatt ataaatggta
     2221 taagtaaaac tacaagtaaa atataatttt tattatcatc aatatagtca aatattttta
     2281 caaatacaac tgtaaaaaat attcctaata tccaacccgc tatcacatct attggccaat
     2341 gaacagctaa atacagccta gacaatccta ctcctaaaac cataactgca cctattaaat
     2401 gaagatatct ctttttaaat atagtcataa tactaaccca aaatgttgtt gcagtttgag
     2461 tatgagcact tggaaaagaa taaccaccag cagtagaaac tctcaatgat tcaagacctt
     2521 ttattcctat tggtctggga gcttttacaa actctttgat gcctaaattt acaacaaaat
     2581 ttccaattat agcaaacagt attttctgtc cacatttttt atttatacac cagtatataa
     2641 ttgttgtaat taatattaat aacggtgctt ctgtgcttat tgtaaagaca agaaatataa
     2701 agttcaaaat tggatttctt aaattttgga aaaataataa tatatttaac tgaatatcca
     2761 tatagatgtc tcctttacta aaattttata taattaacta aaaagcacta actcccttag
     2821 aaattagtgc ttttgattca catttaaatc tattctacat cctcaactat acctatataa
     2881 ggtaaatttc tatacttttc agcatagtct attccataac caacaataaa tttgtcttct
     2941 ataacaaatc caacatagtc tacaggtata ttagcttttc ttctctgagg tttgtcaagt
     3001 agagtacata gttttaaaga ttttggattt ctagttttta aagctgcaac tagattactt
     3061 aatgttaaac cagaatctat tatatcctct acaatcaaca cattctttcc ttcaatatct
     3121 acatctaaat cttttaatat tttaactgtt ccagaagact ctgttgagtt tccataactt
     3181 gtaacactca taaaatctat atttacatcc aaatctatac atcttattaa atctgaaaca
     3241 aatacactag ctcctttaag tatacctacc actagtaaat cttcgccttt aaagtcttct
     3301 tctatctttt ttcctaattc ataaactttt tcttttattt gttcttctgt caacattttt
     3361 cctgtaactt tgtacatttt gtatcctccc gctttccttc tattaactta tatattatag
     3421 cataccaaaa tatcaattaa aatctttttc atcattttta aacctaaatt aacagcttat
     3481 ccatactttt ttgctttttc cattaaaaat ttggaataac gattcctttg tattataatc
     3541 tatatgtaca tatattaatc agttattttt atcgattata taattttatt tttggaggta
     3601 cttttatgag cacaaaccat ggagctaatt tatatagttt atctagtaag tatggctttt
     3661 caaaggaaga atttatggat tttagctcaa atataaatcc ttttggtaca tcaagtttag
     3721 ccaaacaata cattgtaaac aatatagata tggtatctat gtatcctgac cctgattaca
     3781 tagacttaaa aacttctata tccaattact gcaaatgttc tatagataat atagtacttg
     3841 gcagtggagc tacagaacta atttcatcat ttatacatac aattaaccct aaacaggctc
     3901 ttttactctc tccagcctat tcagagtatg aaaaagagtt atctaaaatt aattgttcta
     3961 tagaaaaata ctttgcaaaa gaagaagata attttcatat aaatttagaa aatcttataa
     4021 aaactatcaa tgctaaagat tatgatttgg tagtaatctg taatcctagt aatccaactg
     4081 gatttgcatt tactaaagta gaagtgagag aaattttaaa aaatacagac tcatttttaa
     4141 tgattgatga gacttatgta gagtttactg atacagatac atactcttgt actcaattag
     4201 ttgatgatta ttctaattta ttcgttataa gaggtacatc aaaatttttt tcaacgcctg
     4261 gaataaggct tggatatggt cttatatcta acactaatgt caaaaatgaa ataaataaaa
     4321 atctagattt atggaatata aacataatag cttctaaaat gggtgaaatc atgtttagtg
     4381 atttagattt tatatcaaat actatttcct taatgaacac tgaaagagat tacctgctta
     4441 aagagcttaa gaatataaaa agcttagata tatacaatac taaaggcaat ttcatattgt
     4501 gtaaaatcaa gacaaaggaa ttgactgcta aatctcttcg agaacaattg cttccacaaa
     4561 aaataataat aagagattgt tgttcatttg aaggtctaga tgagtatttc tttagagtat
     4621 gtatattaaa acctaatgaa aataaattat taatatcatc tcttaaagca atttttattt
     4681 taaaaactta ctaaatttct gattatcttt tcataagttg gtaaatgccg atatatcccg
     4741 tattttaggg ctttatcggt attttctttt tggaagatac cttgcataag ctggcattta
     4801 ccagcatgaa aatgcaacca aaagtagtaa atgagtagta aatataagct ccgatattaa
     4861 caagccagtc tttccagttc tgcttttgca gattgaaatg tgccgtgtgc gtaatagcca
     4921 agtgtcatgg ttatgttagc gtgtcccatg agatattgca gagtatttgg gttcattcct
     4981 ctgtttgcca tattcgtaca ataagtatgt cggaatgaat gtggcgtgat gttcggtagc
     5041 ttgtccgtgt gatacttgtt atacttctta atcagccctc gcaccatgcc ctcatagttt
     5101 cctgcaactt taggcaaacc ctcacggttt aagaatagga aattgctgta accacctaca
     5161 atcagcggtt gtgcctttcc tctgttcttt agtattcttt ggattgcttg ataagcccgt
     5221 tctgtcaatg gaagttcccg ttttccgttt ttggtctttg gcgtttcaat atagtagccc
     5281 atttcgctat ctttcaataa ctggtggtct atattgagta ttctgttctg catatcaata
     5341 tgcgtcgtca gtccgcaaaa ttcagaaata cgaagtcccg tttccagcag aagcacaacc
     5401 tcatcataat acttactgta aatcttgtcc ttttccataa aggcaagcag gcgtccttcc
     5461 tgctctggtg taaggataac tttctgttcg gtatcatctt ccagaacatc acttaactta
     5521 aattcaaatg ggttctttct gatacagtcg tcttgtatcg ccatgtaaaa tgacgctttc
     5581 aaggaacgct tatagttatc aatcgtttta taggcatatc ctttttcgct cattctgata
     5641 gcccattctt tagcgtccga ctgcttaacc gtatcaatcg ccctcatacc caatgggtca
     5701 tttttcagag cgttcataag atactgtcgt cctttttcag tagccctttt gatgttcttt
     5761 ctttggctgt tcttcttgtc gtagagctgg cagactgtca ttttaccgcc gactgtgtcg
     5821 ataccgtcgt taaggtcttt ttttatctgt gcttcttttt ccctcaacga tatatcatca
     5881 cgctttccag caggtgtctt gtctgtcggt acaagtttcc aagaatagat aaattgtggc
     5941 tttccgtata tatcggtata tttataaacg tatcttccgt cttttcgctg gctctctcca
     6001 ttacgcaaat tgcgattttt actgtctttt cgtttcacat tagacatagt gtaaagctcc
     6061 tttccgtcat ggaatgagcc gtgatacgct actaatatta taccatatct acggctcaat
     6121 gacattagat ttcgtccaat gtatctatga ttttttcaaa ttgttttcgc ttaatctgaa
     6181 tacgattacc attcacgata acccagccag cgtcaagatt ttcttcggct aatttacgca
     6241 atttcttttc gccaatgcgg aagtatttgg acgcttcttc aatcgtcagc gtatattttt
     6301 cccagatagg cacatcagta ttgttcataa tctatgcacc ccctttactg tgtgtcattt
     6361 tttacaagca ggcagacggc tttggaaagc tccgttagta tctcaactat tcccattcct
     6421 gtgggcagaa ccgcaccatg cggacgtatc attattctgt gaggataggt cgtggcaaag
     6481 cgacttttcc aacggtcgct ctcggatcac tgcgtgggtc gctcgctttc ttttgaaaag
     6541 gtcgtggcgt acagtccccg tggcttgcta tctcacaaac gtatctgtct gctttattca
     6601 gttgtcaaag accagacggg cataatcaat cccgatatga attagaagcg tttaatatca
     6661 aagtttaaga ttttacgaat aatcgcttct ttgataaaac ctttcatttc caaatctacc
     6721 gcaagacgta agttgttgtt atcgtcgtaa aatggacgca tacagcaccg attgatatat
     6781 gggtcataaa attccaagat ttttgaaatt gctgtttcgt cgccgtcgca ggctttggaa
     6841 ataaccgtga aagtcggtgt cagctctttt ggcatttttc tttttcctcc tcaatcagtt
     6901 gctttaactt gtctagtgct tgtattctgc acttcgttac attcgctctg gaacagtcaa
     6961 gcattttccc aatgtaagtg tccgtgtccc caatgaaaaa atactgtaaa acgatattac
     7021 gctttcgttc ttcaatagct ttcaatgcac gagccagttg ttcatcttcg attgatactt
     7081 tttctccaca gacttcaaat accgcaacat aatccgtaga atatgtatct tctgtaccta
     7141 tggaatttaa gaggtcgtcc ataatttcgg cgaatggcat ttcttttttc tctgaacgac
     7201 gtatcgctcg atagaagtca atcgctctat gacgtataac tgttttaata gcacaatcga
     7261 attgatgaca ggttttttct tgtagttctg acggtttcat tttcctcacc tcctttcgct
     7321 agtcgaaaag aggtgtctgc cccttttcat atataccgcg ttgaaagtgg gggacagcgt
     7381 aaccaaaatc gaaaaaactt taaaaaattt ttttctggca tgaaaaaagc ccgcacaaaa
     7441 catatagtct gtacgagctt ttaaaactgt gctatattca gttgttataa aaatgccgtt
     7501 aggttcaaac tgattgcgtc gaggtatatc taaaatattc cataatgaca actcctttac
     7561 ggcactattc taatttaatc agacagaaaa gaaattgcac cgtttttaat ttctaagatt
     7621 tcgtcggctt cttctgccaa atgcttactg tgtgtaacca caacaacaca ttttcctaat
     7681 tcatgtgcag tctttttcag caaagcaatg atttcatcag cggtagtttc gtcaagattt
     7741 cccgtcggtt cgtctgccaa aagtacatga gcgtcgctgg ctaatgctct tgcaatcgct
     7801 acacgctgtt gctgtccgcc agatagttgc aatacatttc tctgccagta ttcttttcca
     7861 atgccgactt cttccaataa tttttcagca tttccactac cacctaattt gacattttca
     7921 accgttgtca aataatcaat cagattataa ttttgaaata ccaatgagat attatgcttt
     7981 ctgtggtaat tcagcccctt ttttgtaatg tcctcgccat tgcaaaggac agtccctttt
     8041 actgctgtgt ccaagcctgc taacaaggat aagagtgttg tttttcctgc accactcgga
     8101 ccgacaatgg tataaaagat accctcctca aaagtagctg atacgttatt caaaatggtt
     8161 ttatcttttt tggttttgta agcatagctt acattcttta cttctaacat tttcgtcgtc
     8221 ctcctaactc atttccgata aaatatcttt cgggtttctc tttagtatta caattccaga
     8281 taccagaaca gaagccgtta tcaacacaag aacacttaca ccgtccaaca gatacatttc
     8341 tggcgtaacc tctacgctga tattctgcac gtcctgcttt ggtgcaacat attctgtgga
     8401 tacttttccc tcgttatttt tttcttcttc ctgcatttgc tgaacctgct gtgataccag
     8461 ataatttgca gttactttgg aaacggcagg agcgacagca aaggatagca taagggaaag
     8521 aacagcaatc ataatcgctt cactccaaat ctgcccgata attctgaatt tcggaacacc
     8581 gagagataag aaaatgccga cttcctgcac tcgatttttc agccagaaaa gaaatactaa
     8641 aacaaggata acaaaacttg caacagagat taccaatatc atcatttcgc ttacttttgc
     8701 taaatcattg aagtttgagg acatggtttc ggaatttccg ttattgtcaa tcaaatcata
     8761 ttgctcccag ttgatgtcta ctttttgcag attttctttt acttcgtcgt aagcttccac
     8821 atcttctact ttgaaatagg ctctttcata taatggactt gtttcaccct ctgccttttc
     8881 tggaaatctc aaatctgtaa atattacatt ttccgaacgg taagtatctc cctgcataag
     8941 tggggacatc ttttgatcca cttgataaat tccaacaatc tcagcttctg atacctttga
     9001 atttacagta tcgtggtaat cattaaaaga aatcttatcg ccgattttca gattattttg
     9061 ctttgcaagt tcctctgaaa tgacacacat atctttatcc tctggggtta tcattcgtcc
     9121 ctctttgata tgcaggtttc cagataaaac atttctgtca agtttcatat ctttgtttcc
     9181 aattaagctt acacctccct catctacact ttggtctaca tctttatctt caattctttt
     9241 gaaatttaca gggtttacag gagttgttgc tgttgtaata ttgtaatcag caattccttt
     9301 tgtttttgcc agtttctcaa tgtcttttat atctaagctt tcaaattcat ttgttgtcca
     9361 gcctgaccaa gtctccacac cattgataat ctctttttcc tgatggtagc caccaaaact
     9421 accttcctta tcattaccaa tcttttctga gtattcacga actcgttttg ttcggtactc
     9481 tgcatttctt tctaaaagaa agcctgcacc tatggcttgt ctggttttgt cctgcacctc
     9541 aatactggca tttttgctcg ccacacctgc aagaagcagg gtactgattg caaaaacaat
     9601 caacaataag agaatacttt ttatgggctt tctgaacaca gaacgccttg ccaatccaaa
     9661 aaaattcatg tactatccct ccattttcgc taatatatct tttgggtttg ctcgtaatat
     9721 cggaagaatg gaacagcaca atgccaccag aataactgca ccacctaaaa ggaacaacat
     9781 tccaatatct tgtatctgca acaccacttt cagattttct gttactgttt ctgaaccagt
     9841 aatgacattt tgcaaaacgc ccgagaacaa tttaccaatg gctgttgctc ctaaaaccga
     9901 aagtgtaaat acactgcctg cttctaataa agtttgcagg aaaatttcac tttttgtctt
     9961 gccgaggctc ataaagattg ccacctcttt ttgccttgtc cgcatccaca tacataacag
    10021 cagggaaaca acaacagtac cagttaccaa tgtaagtacc agcataagat ttgccacctt
    10081 actgatttgc tctaatggag cagacatctg ttgataaagg gtatcggaag tgctaagttc
    10141 tacatcattt ccgaatattt tttgcaagct ggtttccagc acatctaatt tttctggtct
    10201 ttttgaataa acgcagagtt tttcaaattc agcttctttg aacaattctt tataagtgct
    10261 gttgtcaata aagacttgat tttctattcg attgactgcg gtagtttctg ttggctggtc
    10321 tttttccaca tttcctgcgg acatgaaaat tccaataatt tgaacagata cttttgttcc
    10381 gttttctgta cttaattcaa tcgtatctcc aacttttagc tcattttgat ttgcaaggtt
    10441 cgcattgata acagcacctt ttttctcgtg tttgatataa tcgccagaag ataagcggta
    10501 ctgcatttct gaaaaagggc tgtccttttc caaatcatca taggagagca acgctatttt
    10561 caaattttcc tcattcgtag aagtattgag cgtaacggga gcaaaatcat tcggaaatac
    10621 ctcttgtctg ccgatacggt tgatagacga aacatcttcc agcgtttcaa tcttgtttac
    10681 ttcattctct gttatcttgc tgtccttact tgtaatttca gccacaatct tagagtttgt
    10741 cttttcctgc atggcttgtt tggactcatt tgtagtcctt aaaatcatgc ttgtacctaa
    10801 aatcatactg tttacaagta tcaaaacaat caacagaaga atggtttttc ctttttttcg
    10861 ctgtaaatag cgaaaaccaa gttgataaaa cttcatactt cacacctcta ttcccaacgg
    10921 gctatgacaa cctttgttgc attgaaatga agtgtgtctg aaaattctcc atagagatat
    10981 acatttgatt gtttctttac atcggatata gaccccgttg ttatattcgt aacagaaccc
    11041 aactgtttat ttagtgtcgc aacaaaaaat tcacaatctg ggttataggt tacagaaaca
    11101 gcattgtctt tattttccaa tcccgacgct tcgatttgca ggctagctcc accctcagtt
    11161 tcttttgctt gacttacgga acaaccattg tctgtaaatt ccaaaacact tcctatcaaa
    11221 ttagcagact ctaatatgtc gtttccttta ttttctgaag aaccatttgc attttcttca
    11281 ttcgtattag aattgacaat aggattagac gtatctggtt tctcatctgg acttgcacca
    11341 caccccgtca atgcacagat acataatcca actgctacaa atttactgat gtttgttttt
    11401 ttcatttttg tattccacct ttcttgttcg atatgcccct attgtaaaag caaaccattc
    11461 taaaaatcct gtaaaaaagt aagaaaatca tcatcatttt ttagaggatt tttacagact
    11521 tttctgttat actgtctata atttatatag aaaaggattg atacattatg aaaatacttc
    11581 ttctggaaga tgatttagag ttatgtaaaa atataaaaat ttctctcgaa aaagaaaact
    11641 atatggttga ttgttgcaat gacggggaaa ccgctatgct gtacgcttta aatacagact
    11701 atgcttatga cctcgccatt gtagaccgta tgctaccgat tattgacggc ttaacaatca
    11761 taaaatctat gcgtagaaaa ggaatatcta ttcctatcct tatcattact gccatgagta
    11821 cccttgacaa tcgggtggag ggtttagacg gcggtgctga tgattactta gtaaagccat
    11881 ttcatatcaa agaattgctt gcaagagttc gggcattaac aagacgccct gccaatatcc
    11941 agttatcttc caaattagaa tatggagatt taactttcga taaagacagc cgaaccttat
    12001 ctacacccgt caattctctt ttattaactt caaaagaaac cgagttgact tttgtattta
    12061 tgcagaaccc agaaacacta ttttcccgtg aacagctaat ccttaaaatc tggggcagtt
    12121 cttccgaggt agaaactggc aatgtagaca gttacatttc ttttctgcga aaacgcctaa
    12181 aagaattaaa aagttcctgt aaaataacaa cggtctacgg tgcagggtac aaactggaga
    12241 aagaaacatg ttaaataact tataccgaaa acttcatatt ctgtttgcaa gttctgttat
    12301 gctgattatt acccttgtga tttcctttgt cgttgcgaac acagttcata cagagaaaat
    12361 caacgaaagc accttatttc agagattgac aaccttgctg atttatcaag tggaaagtgc
    12421 ttcttcggat atggacaaag cactcaaaac ctacgaagag agttatcata ttttttcttt
    12481 aattactgat acaaggggga ataccattta tcagagtgat ttcccatttc caacttctgc
    12541 ggacaatctg ttgcatgatg tagaaaagca gatttctaca caaccattat cacaaataga
    12601 aaacagcaca acttcacaag gcggttttct ggaaataaga ggaacacatc acgatactta
    12661 ttttgttatt cctgctacaa ttaggacagc aaatgatacc gtgtatcatg caacctttct
    12721 ttatcagaca gccagtttga cggatatttt acaaaaaaca ttgcctatat atcttcttat
    12781 ctggtttttg gcttgtatcg ttgttatcgt gctgacccgt tatctgctga aaaagtcgtt
    12841 tgctccaacg gaacgaatac tgcaaagcca aaaagatttt gttgcaacag cgtcccatga
    12901 attaaaatct ccacttgctg ttatgatttc caacacggat atgcttcttg ataatgtttc
    12961 cttaaacgag caggcaaagc aagccgtaca gaccatagat tttgaatgta tgcggttatc
    13021 ccgtttagtc aaagatatgt tgcttttggc ttcttccgac gctaaaacat ggacattgca
    13081 taaaagcaca atcaatgtag ataccttgct cattacgtta tatgaaacct acgaacccgt
    13141 ctgcatgaag cagaatttag aactaaaact gcatttatcc gaggaaagtt atcctgctat
    13201 gtttaccgac aaagaccgat tatttcaaat cttgtgtgtc tttatggata atgccattca
    13261 acattccaag aataattctt taatagagat agaagtaatc gccaccgaaa aaaatattgc
    13321 tttttctgtg gtagaccacg ggcaaggcat ttctgatgaa gataagcagt atatctttga
    13381 ccgattttat agtggcgaca agtcccatac gaacaaatcg aattttggac tgggactaag
    13441 tatcgccaaa gaattaactc aaatgctgaa cggtattatc attatcaatg atactgctgg
    13501 tggaggtgct acttttactg ttaaatttcc tctgaaatag attaaggctt gcagatcata
    13561 cactatgtat ggagcaagcc ttttttcaaa tctttgtctt tttggtaatc ttgtaaatat
    13621 attcttctgg ggtagggcgt ttattaccaa aatacttttt gaaatttgcc tgcacttctg
    13681 gggcggtgct attcatagct tcatcaatcg tcgcttcaat atctgcccga acatcagcca
    13741 atgatacacc gccagctttt gcaccagctt tcaacgcttt ttttatatct cgctttttta
    13801 gtcctatcat aataaccttt cttatcataa ttgcaaattt ataaattcat tagtattaaa
    13861 actgaaattc aaatacatta ctaaaatcat tgttcaagac ataataaact ttattttttt
    13921 ctgtagaata tacaaatgaa accctagttg cccacaagtc tttttgagaa acagatttta
    13981 atactttaaa tgcgtcattt actgaaaaat ctttatcata tcctgccaat aactctttag
    14041 ccacttcgta tcttgtatcc ccagaaacaa tgtacggctt tgttatatct ggttttagaa
    14101 tagaataatt tgtaataagt gaaaaatttt cttgttcata tctataccca attccaggtt
    14161 caataattaa aactcgtcct tttttatcag aaagcatagc ttgcatagta gcatctggtg
    14221 cataaataat ttttttcttt ttaacaatgt ccaaggcttt atcaaaagaa atctctgcct
    14281 taatgaattt ttctgttaat tcagatattg taatacaatc ctcgctttct atataccttg
    14341 cattttcatt accatgaaca taaagaagtg ttcctacatt accattttta tttatcccat
    14401 gaaatgaatg atacagaccg tcaggcattt taataccaat ataaaatcta ttttcttcgg
    14461 taattacagt atgatcccat acagaaaggt caatatcaaa attaaaacca acaattttat
    14521 ctataccatt gtaaacaaat cttgtacaca tataatcact cctcaaaata ctattttaaa
    14581 tagtcattta actaatctaa ttagtatttt aaaatgtttt tgtaagaaag tcaataaata
    14641 atcataattt tctaattgtg ttattgacta cactaattca attagctaga attgtgtttg
    14701 tatgatattt tgaatgaggt gcataactat gaaaaccact aaagaagcag taataaaagc
    14761 aacatcagac ttagcagatg aaaaaggatt aaataacgtt tcgttaaaag ctgttgctca
    14821 aaaattaaac attcgtacac catctttata taatcacata gaaagtttag ataatcttct
    14881 tttagaagtt gctcataatg gtatgcgtga catgaatgaa agaatgatga aaattgccgt
    14941 tggaaaaata gggaaagaag caattaagtt agtttctatc gaatacctaa actatatgat
    15001 tgaacacccc ggagtttatg aaacaattca atgggctgtg tggcacggaa cagaggaaac
    15061 agcaaccatt tttaacaact atctttcatt attaactact cttatccaat cgtgcagtct
    15121 taacaaagac aaaacacttg aaatattaaa tatgcttact ggaataattc acggttatac
    15181 aactttgcaa ttaggaaacg ctttttctgc ctcagataaa gttagatttg aattagctga
    15241 agcgatagat actttattag tcgggatttt ccagaagtat aaataatata tttaggcttg
    15301 caaatcaata cagaactgca agcctttttc ttttatccta caatcttcca gttgctatcc
    15361 ttatgtagca caagctcata ctgtgatacc tgcgtcgcct ttgtctgatt atcaaggaat
    15421 tttaccgcta ccttgacttt cacattatcc ccgttctttg taaagatagg gtttaccagt
    15481 tcagaataaa ggtagtccct gccgataggc tcaatcacat ttccagatac atagtaagca
    15541 agctcttttt ctgtggctgt tggatagagc ttaaagaatg tttccagaaa tgcggtagcg
    15601 tcatttaccg tatcagcgtc cacgcttgcg tctgcttctg gtgtcttagg ctcatagtct
    15661 gatttttcga ttgctggtgc aagagtaggg ttctgaatga ttaccatatc cccgtcagcg
    15721 tctacatgga cttttacagt ataagtcgct ttcacattgc ttgtctgttc tccctctttt
    15781 atctgctgat ctacttcgta ggtagcagaa aaagtgtccg ttcccgattg ctcgatatgc
    15841 cacacaatca catctgtaac tgtggagctg gtcggtatat ccgttctgat tgtatcaaca
    15901 ttcaagtctt gcaattcctt tgtcagataa ccgctgattg cctgcgtcct tgcttcaatc
    15961 gcttctttgc tgttactcca tgtgtaataa gacttcgcaa agttcttcac gaaattttct
    16021 atcccgttgg tgtcctgcaa acgaagttca atgatttctt tttcatgggt cgtgtgctgg
    16081 tcgatagccg taaaattctt atacacccca aagctcacgc ttgcgataag caccacccac
    16141 aacgcaatca tggttttctt atgtgtgcct accttgacag tacgcacctt tttttctttt
    16201 ggttctttga cagtttctgt ctgtttctta tttttcttaa acatattttc aagtcctttc
    16261 tattgtttta ctcgtcctgc accgattaag tgctgttgcc agtaactact acttaggtct
    16321 gcatatccta tcgggtcgcc tgcatgatac atctgattat ttcc
//
LOCUS       DACULQ010000084        16350 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.084, whole genome shotgun sequence.
ACCESSION   DACULQ010000084 DACULQ010000000
VERSION     DACULQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.084"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..369
                     /gene="tuf"
                     /locus_tag="JA154_10725"
     CDS             <1..369
                     /gene="tuf"
                     /locus_tag="JA154_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086521.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAT7996336.1"
                     /translation="DNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEE
                     GGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLR
                     FSIREGGRTVASGVVATIIE"
     gene            619..768
                     /gene="rpmG"
                     /locus_tag="JA154_10730"
     CDS             619..768
                     /gene="rpmG"
                     /locus_tag="JA154_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262850.1"
                     /note="in Escherichia coli BM108, a mutation that results
                     in lack of L33 synthesis had no effect on ribosome
                     synthesis or function; there are paralogous genes in
                     several bacterial genomes, and a CXXC motif for zinc
                     binding and an upstream regulation region of the paralog
                     lacking this motif that are regulated by zinc similar to
                     other ribosomal proteins like L31; the proteins in this
                     group have the CXXC motif; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAT7996337.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            794..1015
                     /locus_tag="JA154_10735"
     CDS             794..1015
                     /locus_tag="JA154_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAT7996338.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            1035..1577
                     /locus_tag="JA154_10740"
     CDS             1035..1577
                     /locus_tag="JA154_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAT7996339.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1616..2041
                     /locus_tag="JA154_10745"
     CDS             1616..2041
                     /locus_tag="JA154_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAT7996340.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            2112..2810
                     /locus_tag="JA154_10750"
     CDS             2112..2810
                     /locus_tag="JA154_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAT7996341.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2862..2990
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3033..3539
                     /locus_tag="JA154_10755"
     CDS             3033..3539
                     /locus_tag="JA154_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAT7996342.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3597..3962
                     /gene="rplL"
                     /locus_tag="JA154_10760"
     CDS             3597..3962
                     /gene="rplL"
                     /locus_tag="JA154_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086528.1"
                     /note="present in two forms; L12 is normal, while L7 is
                     aminoacylated at the N-terminal serine; the only multicopy
                     ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two
                     L12 dimers bind L10; critically important for translation
                     efficiency and fidelity; stimulates GTPase activity of
                     translation factors; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAT7996343.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            4354..5142
                     /locus_tag="JA154_10765"
     CDS             4354..5142
                     /locus_tag="JA154_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT7996344.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5613..9329
                     /locus_tag="JA154_10770"
     CDS             5613..9329
                     /locus_tag="JA154_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAT7996345.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIVKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9371..12856
                     /locus_tag="JA154_10775"
     CDS             9371..12856
                     /locus_tag="JA154_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAT7996346.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            13145..13567
                     /locus_tag="JA154_10780"
     CDS             13145..13567
                     /locus_tag="JA154_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAT7996347.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13691..14161
                     /locus_tag="JA154_10785"
     CDS             13691..14161
                     /locus_tag="JA154_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086533.1"
                     /note="binds directly to 16S rRNA where it nucleates
                     assembly of the head domain of the 30S subunit; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAT7996348.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            14210..16276
                     /gene="fusA"
                     /locus_tag="JA154_10790"
     CDS             14210..16276
                     /gene="fusA"
                     /locus_tag="JA154_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086534.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAT7996349.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6411 a         2033 c         3339 g         4567 t
ORIGIN      
        1 gataatatag gagcattatt aagaggagta caaagaaacg agatagaaag aggacaagta
       61 ctagcaaaga ctggatcagt aaaggcacac acaaagttta cagcagaagt atatgtactt
      121 aaaaaagaag agggtggaag acatacacca ttctttgatg gatatagacc acaattctat
      181 ttcagaacaa cagacgtaac aggagcttgt aagttaccag aaggaataga gatggtaatg
      241 cctggagata acgtaacaat ggaagtagac ttaataaact caatagttgt agaagaggga
      301 ttaagattct caataagaga aggtggaaga acagtagctt caggagttgt tgctacaata
      361 atagagtaat tctttactaa actgatggat taaaaggaat aacaaaaaga ggggttatgc
      421 tcctcttttt ttaacataga aatacaaaaa cattgacaca gcataggatt ttgtgataac
      481 ttatacaagg taatgattat aggaagttaa taatatcttg aatactgtag atttttaata
      541 aatacatatt ttaaagatat tatattcaca tatattaaca atatatatta ttgtattaca
      601 aagttggagg tgtatcagat gagagttaaa gtaactttag catgtacaga gtgtaagcaa
      661 agaaactaca acactactaa gaataagaaa aataatccag acagaataga attacaaaaa
      721 tattgtagat tctgtaaaaa gcatacaact cataaagaaa caaaatagtt ttttataagg
      781 atgtgagtat ggaatggctg cccaaaaaaa tgacggtgca aaaactaaaa aaaggtttag
      841 tttgtttgga tacttaaaag aaactaaaca agaattaaaa agagttacat ggccgacaaa
      901 aaaggaactg tttaaaaaca caagtatagt tttaactgta gttatttcat gcactatatt
      961 agtatggggt atagacacta tattatctgg tgcactagcg ttattactaa aatagacgaa
     1021 aagaggtaag ttaaatgtca gaattacaag aagcaagttg gtatgtagtt catacctatt
     1081 cgggacatga aaataaagtt aaagctacaa ttgagaaagc agttaaaaca agaggtatgg
     1141 aagactgcat aagacaagta gttgttccaa cagaagaagt agtggaaact actaaaactg
     1201 gaaaagaaaa aactagacaa cgtaaagttt atccaagtta tgttttggtt aaaatgataa
     1261 taactgatga atcttggtat gtagtaagaa atactaaggg agttacaggt ttcgttggac
     1321 ctggttcaaa accagtgcca ttaagtgaag acgaagtcaa agctatgggg atagacacaa
     1381 cagaccctaa agtagtaaat agcgatattg attttgaaat aggagataca gtaaaagtat
     1441 ctcaaggacc atttagtgga caaataggta acatagaaga aattgactta gaaaatagag
     1501 aagtcaaagt atgcataaat gcatttggta aaaggactct atttgtaata gagcttgaag
     1561 gtatagaaaa aatctaatac tatattatag aaaaatctga ggaggtgcgc tcaaaatggc
     1621 taaaaaagtt ataggtcaaa taaaattaca aatacctgca ggaaaggcta ctccagcgcc
     1681 accagttgga ccagcattag gacaacatgg tgttaatata atgggattca caaaagaatt
     1741 taatgctaaa actgcagatc aagctggaat gataatacca gttgttataa ctgtatatca
     1801 agatagatca ttcagtttta taacaaaaac tccaccagct gcagttttaa ttaaaaaagc
     1861 attaaactta aaatcaggtt ctggagaacc aaacaagaaa aaagttgcta aaatgacttc
     1921 agctcaagta agagaaatag ctgaattaaa gatgcctgac ttaaatgctg catcagtaga
     1981 agctgctatg agtatgatag ctggtactgc tagaagcatg ggtgttgtaa tcgaagacta
     2041 gttaaaagat acactttttt ttataaagtg ggaggctaga agccgaaatt aaccacgagg
     2101 aggaaaataa aatggctaaa aaaggtaaaa gatatgcagg tgcattacaa aaagtagata
     2161 gaactaaatt ttatgatgca tcagaagcat taacattagt ttcagatata gctggagcta
     2221 aatttgatga gacagtagaa gcacacatta aattaggtgt tgactcaaga catgctgacc
     2281 aacaagtaag aggtgcagtt gtattacctc atggaactgg taaaacaaaa agagttttag
     2341 tttttgctaa aggtgaaaaa gctaaagaag ctgaacaagc tggtgctgac tttgtaggag
     2401 ctgaagaatt agttcaaaaa atacaaggtg aaaactggtt tgactttgat atagtagttg
     2461 ctactccaga tatgatgggt gtagtaggta gattaggtag agtattagga cctaaaggtt
     2521 taatgccaaa ccctaaatca ggaacagtta catttgatgt agctaaagct atagatgaaa
     2581 taaaagctgg taaagttgaa tacagattag acaaaactaa cataatacac gttccagtag
     2641 gaaaagtatc atttggtgga gaaaaattaa ctgaaaactt tactgcatta atggatgcta
     2701 taataaaagc taaaccagct gctgctaaag gacaatattt aagaagtata acagtagctt
     2761 cttcaatggg acctggagtt aaaataaacc cagcaagaac agctgaataa taaaactgtt
     2821 gacacaggtt gacaaaaatg ttattatatc acttgtcaat gtaaaaaaga atagatttgc
     2881 cgtagatagt gggtgcgaaa gcttaatatc ccgccgaggt tttagataaa ttgagattac
     2941 taaagctttt gatgtctact tttggcaaca aaagcttttt ataatgttgc ggtaaatact
     3001 aagcctattt ggtgaaggag gtgcacatct atatgagaaa agctatagaa attaaatcag
     3061 aagttgtttc tgagatagtt gaaaagttac aaaaatcttc tgctgctgtt gttgtagatt
     3121 acaaaggatt aacagttgaa gaagtaactg agttaagaaa acaaatgaga gaagctggtg
     3181 tagattacaa agtatacaaa aatactttag ttagaagagc agctaaagaa gttggtatag
     3241 agcaatttaa tgatgaatta ttagttggaa ctaatgccat agcatttgga tatgatgatc
     3301 cagttgctcc agctagaata ttaaaaggat tcatggattc tcatccaaaa atgaaattaa
     3361 agatgggtat tgtggaaggt gcattctatg atgaatctaa gattgttgaa atggctaaca
     3421 taccatcaag agaagttctt atcgctaaat tacttggtag cttaaaagct ccagtatcaa
     3481 acttcgcata cttaatagat gcaatagcga aaaaggcaga aggtcaagaa gaagcttaat
     3541 tgcttaacta aaaaattata aatattaatt tgaaataaaa ttcggaggtg ctaaaaatga
     3601 caatagaaca aatattagaa gctatagaaa atatgaaagt tttagaatta aatgaattag
     3661 ttaaagctgc tgaagaaaaa ttcggtgtat cagcttcagc tccagtaatg gtagctggtg
     3721 cagctgctgg tggaccagct gctgaagaaa aaactgagtt tgatgtagta ttaactgacg
     3781 ttggttcttc aaaagttgga gttataaaag cagttagaga aataacagga ttaggattaa
     3841 aagaagctaa agaagtagtt gacaatgctc ctaagacagt taaagaagga gcttctaaag
     3901 aagaagctga tcaaataaaa gagaaattag aagctgctgg agctaaagta gaagttaagt
     3961 agtttcttta aaaagatacc cattgggtat ctttttttat ttataaaaaa ctataaagaa
     4021 agtatttgga attataaaga aagtattttt ataaaataaa aaaaatttat ataatagtac
     4081 taaattaagg gtatcatata tggtgtaatg gtattttaat ttaacattat tatcaaatta
     4141 aaatataaac aattgttgta atgtctatat ataaagtata taattaaact aaaatgtaaa
     4201 attttacatt ttagtttaaa aaaatacacg aatgttttat aaataaaatg taataaataa
     4261 ttaattaaca aatataaatt ttaaaataag ttagatattt agaaaaatcg aaatttgtag
     4321 gatttaaagg acatattcaa ggaggagtta ataatggaaa aattacaagg aaaaattgca
     4381 gtagttactg cagcaacaaa aggtattgga ttagcatcag cagagatatt agcaaaaaat
     4441 ggagcaactg tgtacttagc agctcgttca gaagaattag ctaatgaagt tataaataaa
     4501 ataagtgcag aaggtggttg tgctaagttt gtttacttta atgctcgtga agaagagacc
     4561 tttacctcaa tgatagaaga ggtagttaaa aaagaaggta agatagatat acttgtaaac
     4621 aattttggtt caacaaaccc ttctcttgac aaagaccttg taactgggga tacagataat
     4681 ttctttgaca cagtaaatac taatttaaaa agtgtgtatt taccatgtaa agcagcaatt
     4741 cctcatatga taaagaatgg aaaaggaagt atagtaaata tatcaagtat aggttcagta
     4801 ttacctgatt tatctagaat agcttactgt gtatcaaaag cagcaatcaa ctcattaact
     4861 caaaatatag ctacacaata tgctaaagac aatgttagat gtaatgctgt acttccaggg
     4921 cttattgcaa ctaaagcagc attagacaat atgtctccag agttcataaa agaattttta
     4981 aagcatgttc ctttaaatcg tataggggaa ccagatgata tagcaaaagc tgttctattc
     5041 tatgctagtg atgactcatc atttataaca ggggatttac ttgaagttgc aggaggattt
     5101 ggtttaccta ctccacaatt tgcagataat atattaggat aattaaatta ctaatatgca
     5161 atgaaagctt atgcattagt tatagtagaa atgatatttt tataaaggcc atagatataa
     5221 tttatattgg aaaggtttga ggttcaaaat tccaagttaa atttatttat ggtctttatt
     5281 ttgtatattt taataaattg tatatatttg tataaatgta attatttaca atcattttaa
     5341 tatggaatta taattaaata ggggggatgg tacttgtata aaatttttta tagaaaatta
     5401 gacctataaa tgaaaaatat ggattttata ttggaaaatt gtacataata cataatatat
     5461 gcttaaaata atttacaaaa aatcaaaaat aatgcatagt ttacatataa gataaaaaat
     5521 atattgaaaa atacttgaca ttatgctaaa attattagat gcgttagaat gaactattgt
     5581 tttattttta attattgaga ggtgaaaagt gaatgccaca tcctgtcacg ataggtaaaa
     5641 gaactagaat gagcttttct aaaattaagg aaatagctga tgtgccaaat cttatagaaa
     5701 ttcaagtaga ttcctatgag tggtttttaa aagaaggttt aaaagaagtg tttgatgata
     5761 tttcaccaat agaagattat actggtaatc ttatattgga atttgtcgac tattctctag
     5821 atgataaacc aaagtacgat atagaagagt gtaaagagag agatgctaca tattgtgcac
     5881 ctctaaaggt taaggtaaga cttataaata aagaaacagg tgagataaaa gaacaagaag
     5941 tgtttatggg tgacttccct ttaatgacag aaagaggaac tttcgttata aatggagcag
     6001 aaagagttat agtaagtcaa ttagttagat ctcctggtgt ttactatgcg gaagaaagag
     6061 ataaaacagg taagagattg atttcatcta ctgtaattcc taatagagga gcatggttag
     6121 aatatgaaac agattctaat gatgtaatat ctgtaagagt tgatagaaca agaaaacaac
     6181 cagttacagt tttacttaga gctttaggaa taggaacaga tgcagagata attgaccttt
     6241 taggagaaga tgagagatta tctgctacat tagaaaaaga taatactaag acagtagaag
     6301 aaggtcttgt agagatatac aaaaaattaa gaccaggtga acctcctact gtagaaagtg
     6361 catcatcttt attaaatgct ttattctttg acccaaaaag atatgaccta gcaaaagttg
     6421 gtagatataa attcaataag aaacttgctt tatgttacag aataatgaat aaaatttcag
     6481 cagaagacat aataaatcca gaaactggag aagtatttgt aaaagctgga gaaaaaataa
     6541 gctatgattt ggctaaagcc atacaaaatg caggtataaa tgtagtaaat ctactaatgg
     6601 atgatgataa aaaagttaga gttataggaa ataattttgt ggatattaag tctcacattg
     6661 attttgacat agatgattta aacataaaag aaaaagtaca ttatccaact ttaaaagaaa
     6721 ttttagatgg atatagtgat gaagaagaaa taaaagaggc tataaaatca agaattaaag
     6781 aacttatacc aaaacatata ttattagatg atataatagc ttcaataagt tatgagttca
     6841 atatattcta taatatagga aatattgatg atatagatca cctaggaaac agaagaataa
     6901 gatctgtagg tgaattgtta caaaatcaag ttagaattgg tctttcaaga atggaaagag
     6961 ttataaaaga aagaatgacg gttcaagata tggaagctat aacgcctcaa gcgttagtta
     7021 acataagacc agtttcagct gcaataaaag aattttttgg tagttctcag ttatctcagt
     7081 tcatggatca aacaaatcct ctatctgagt taacgcataa gagaagatta tcagcccttg
     7141 ggcctggagg tctttcaaga gaaagagctg gattcgaagt gcgtgacgtt caccattcac
     7201 actatggtag aatgtgtcca atagagactc cagagggacc aaatatcggt cttataaact
     7261 ctctaggaac ttatgcaaaa ataaatgaat ttggatttat agaatcacca tatagaaaat
     7321 ttgataaaga aacatcaaca gttactgatg aaatacatta tttaactgct gacgaagaag
     7381 atttattcgt aagagctcag gcaaatgagc cattaacaga agatggtaaa ttcgtaaacc
     7441 atagagttgt ttgtagaact gtaaatggtg ctgttgaaat ggttcctgaa agtagagtag
     7501 attacatgga tatatctcct aagcaggttg tatctgttgc cactgctatg atacctttct
     7561 tagaaaatga tgatgccaac cgtgccctta tgggagcgaa catgcagcgt caggcagtgc
     7621 ctctagttag aagagaagca ccaattatag gaactggtat agaatacaga gcagcaaaag
     7681 actctggagc agttgttgtt gctagaaatt ctggtatagc agaaagagta acagcagatg
     7741 aaataattat aaaaagagaa gatggaaaca gagatagata taatctactt aagtttaaac
     7801 gttcaaactc aggtacttgt ataaatcaaa cacctataat aaataagggt gaccagatag
     7861 taaaaggtga tgttatagca gatggtccag caactgattt aggagaagtt gcacttggaa
     7921 gaaactgtct tatagcattc atgacttggg aaggttataa ctacgaggat gctatattaa
     7981 taaatgaaag attagttaaa gaagatagat tatcaacaat tcatatagaa gaatatgaat
     8041 gtgaagctag agatacaaaa ctagggccag aggaaataac tagagatata cctaatgttg
     8101 gagatagtgc aattaagaac ttagatgaca gaggtataat aagaataggt gcagaagtag
     8161 actcaggtga tatattagtt ggtaaagtaa ctccaaaagg agaaactgaa cttactgctg
     8221 aggaaagatt acttcgtgca atatttggag aaaaggctag agaagttaga gatacttcac
     8281 ttaaagtgcc tcatggtgaa tctggtataa tagttgatgt aaaagtattt actagagaaa
     8341 atggagatga tttatctcca ggagtaaatg aattagtaag atgctatata gctaaaaaga
     8401 gaaaaataaa agttggagat aaaatggctg gtcgtcatgg taataaaggg gttatctcaa
     8461 gagtattacc tgaagaagat atgccattca tggaaaatgg aacaccacta gacataatac
     8521 ttaacccaca aggtatacca tcacgtatga acattggtca ggttttagaa gttcatttag
     8581 gacttgctgc taagacatta ggatggtatg tagcaacatc agtattcgat ggtgctaatg
     8641 agtatgacat aatggatgct cttgaagaag ctgggtatcc tagagatggt aaattaactt
     8701 tatatgatgg tagaacaggt gagtcttttg acaacagaat aactgttgga tatatgtact
     8761 acttgaaact acatcactta gtagatgaaa aactacatgc aagaagtact ggaccatact
     8821 cattagttac tcaacagcca ctaggtggta aagcacaatt tggtggacaa agatttggag
     8881 agatggaggt ttgggctctg gaagcatatg gagctgctca catacttcaa gaaatactta
     8941 ctgttaagtc tgatgacgtt gtaggtcgtg ttagaacata tgaagctata gttaaaggtg
     9001 aaaatatacc tgaaccagga atcccagaat catttaaggt tcttataaaa gaacttcaaa
     9061 gtttatgctt agatgtaaaa gtattgacag atgaagacca agaaatagaa gtaagagaat
     9121 ctgtggatga agatgataca ataggtgaat ttgagctaga tgttgtaaat catatgggag
     9181 aagttgaaga aagtaatata atagaagaaa ttgaagatga ttttgcagaa aatgcagaag
     9241 atgaggatat agagaattta gaagaattca ctgaagatga tttatttgaa gaagaaatag
     9301 attttgatag tgatgacttt gatatgtaaa tttaattaat gtaaatctca aatagaaggg
     9361 agagaactcc ttgtttgaat taaacaattt cgagtcgata aaaatagcat tggcttctcc
     9421 agaaaaaata agacaatggt ctaggggaga agttaaaaag ccagaaacta taaattaccg
     9481 tactttaaaa ccagaaaaag atggtctttt ctgtgaaaga atatttggac cacaaaaaga
     9541 ctgggagtgc cactgtggta aatatagaag agttagatat aaaggtgtag tttgtgatag
     9601 atgtggagta gaagtaacta aatcaaaagt aagaagagag agaatgggac atatagagct
     9661 agcggctcct atgtctcaca tctggtactt caaaggtata ccaagtagaa tgggactttt
     9721 acttgatatg tcaccaagat cgttagaaaa aatattatac tttgcctcat atgtggtagt
     9781 tgatccagga gagactggat taaatgaaaa acaattgctt acagaaaaag aatacagaac
     9841 tgctcttgaa aagtatggat atacttttac tgtaggaatg ggtgctgaag ctgtaaagac
     9901 attactacaa aatatagatt tagagcaaca aagtaaagac ctaagagcgg agttaaaaga
     9961 tagtacaggg caaaagaaag ttagaacaat aagaagatta gaagttgtag aggcatttaa
    10021 aaagtctgga aataaaccag aatggatgat tttagatgca ataccagtaa taccaccaga
    10081 tttaagacca atggtacaac ttgatggtgg aagatttgcg acttcagacc taaatgattt
    10141 atatagaaga gttataaata gaaacaatag acttaaaaga ttattagagc ttggagctcc
    10201 agatataatt gtaagaaatg aaaaaagaat gcttcaagaa gctgttgatg cattgataga
    10261 taatggtaga agaggtagac ctgtaacagg acctggaaat agaccactta aatctttatc
    10321 agatatgtta aaaggtaagc aaggtcgttt ccgtcaaaac ttacttggta agcgtgttga
    10381 ctactcagga cgttctgtta tagttgttgg accagaactt aaattttatc aatgtggact
    10441 tccaaagaag atggcattag aattattcaa gccatttgtt atggataaat tagttaaaga
    10501 aggttatgca cataatataa aaagtgcgaa atctatagta gaaaaagtta agccagaagt
    10561 ttgggatgtt ttagaagatg ttataaaaag tcatccagtt cttcttaacc gtgcaccgac
    10621 tctacataga ttaggtatac aagcatttga accaatctta gttgaaggta aggctataaa
    10681 actacatcct cttgtatgta cagcttataa tgcagacttt gatggtgacc aaatggcagt
    10741 tcatgtacct ttatcagtag aagcacaagc agaagcaaga ttcttaatgc tttctgtaaa
    10801 taatatactt gctcctaaag atggttcacc tataactact ccatctcagg atatggtttt
    10861 aggttgctat tatctaacaa tagaagccca agatggagct aaaggaacag gtatggtatt
    10921 taaagacttt aatgaactat tacttgctta ttacaataaa tcagttcatc tacatgcatt
    10981 agtgaaactt aaggtaacac ttgaagatgg aagaagttca ttggttgaaa gtactgttgg
    11041 tagatttata tttaatgaaa atatacctca agacttaggt tttgtagata gaaaagaaaa
    11101 tccatttgca cttgaagttg atttcttagc agataaaaaa tctcttggta aaataataga
    11161 taaatgcttt agaaaacacg gaaatacaga aacagctgaa ttactagatt atataaaagc
    11221 tctaggattt aaatattcta cattaggtgg cataacagtt gctgttgatg atatgagtgt
    11281 cccagaagaa aagaaagtat ttatagctga ggcagaagct aaagttgata agtatgaaaa
    11341 agcatacaga agaggtctaa tctctgatga agaaagatat gaaaaagtta tagagacatg
    11401 gacagaaaca actgataaag ttactgatgc tcttatgggt ggactagata gattaaataa
    11461 tatatatata atggcacatt caggagccag aggttctaaa aaccaaatta gacagctggc
    11521 aggtatgcgt ggtcttatgg caaatgcatc tggtaaaaca gttgagatac cagttaaatc
    11581 taatttccgt gaaggtttat cagtactaga atactttaca tcttcacatg gtgccagaaa
    11641 aggtcttgcc gatacagcta tacgtacagc tgaatctgga tatttaacaa gaagacttgt
    11701 tgatgtaagt caagatgtta ttgtaaggga aatagattgt ggaacagaag atactacaga
    11761 gatttatgct ataaaagaag gaaatgaagt tatagaagag atatatgata gaattgtagg
    11821 aagatatact atagacccta tattaaatcc tgaaactgga gaagttatag ttgaggctga
    11881 ttcaatgata caagaagatg aagcagaaac tatagtagct ttaggaattg aaaaaattag
    11941 gataagaaca gttcttaact gtaaaactaa tcatggagtt tgttctaagt gttatggtag
    12001 aaacttggca acaggaaaag aagttaatat aggtgaagca gttggtataa tagcagctca
    12061 atctatcggt gagccgggta ctcagcttac aatgcgtaca ttccatactg gaggagttgc
    12121 aggagctgat ataactcaag gtctaccaag ggttgaagaa ttatttgaag ctagaaaacc
    12181 aaaaggatta gctgtaataa ctgaagtatc tggtagagtt gaaattgatg aaacaggaaa
    12241 gagaaaagaa gtaaatgtaa taccagaaga aggcgagact caaacatatg taattccata
    12301 tggttctaga ttaaaagtta agcaaggtca aatgctagag gctggagacc ctctaacaca
    12361 aggatttata aatcctcatg atatagtaag agtaaatggt gttaagggag ttcaagaata
    12421 tatagttaaa gaagttcaaa gagtgtatag acttcaaggg gttgatgtta atgataagca
    12481 tatagaagtt atagtaagac aaatgctatc taaagtaaaa gttgaagacc caggagatac
    12541 agatttattg ccaggtggat atgaagatgt attaacattc aatgaatgta ataaagatgc
    12601 tatagataaa ggtctaagac cagcagttgc taaaagagtt ttacttggta taactaaagc
    12661 atctcttgca actgattcat tcttatcagc agcttctttc caagaaacaa caagagtatt
    12721 aacagaggca gcaataaaag gtaaagaaga ccacttaata ggacttaaag aaaatgttat
    12781 attaggtaag ttaataccag caggaacagg aatgaagaaa tatagaaata tagctgttga
    12841 aaaaattgaa gattaatagc atatagagta aagaaatctt gacaaccatt atttgtggtg
    12901 ttaaaatagt aaagtgtgtt gtttaaaagt acactaaggg cttgaacaag ttcttagtga
    12961 cttttagatg gcaataaatg agaactaaaa agcatcaagc tagaaaagtg ctaatatcac
    13021 atatttatgt gttatagaca ttaaattgta agtaacgaat gttacaatca gtcagtatga
    13081 atattcatat tgatgtagtt ggcggtcact gataagatcg aaaatttgga aggaggtgca
    13141 gatgatgcca acaattaacc aattagttcg taaaagtaga aaagcgctag agaagaaatc
    13201 tactgctcca gcattacaaa aaggttacaa ctctttaaat aagaaagtaa ctgacgctag
    13261 tgccccacaa aaaagagggg tttgtacttc agtaaaaaca gttactccta agaagcctaa
    13321 ctcagcttta agaaaagttg ccagagttag attaactaat ggtatagaag tttctgctta
    13381 tataccagga gaaggacaca acttacaaga gcatagtgtt gttcttataa gaggaggaag
    13441 agtaaaagac cttccagggg ttagatacca tatactaaga ggtactctag atacagcagg
    13501 tgttgataag agaagacaat caagatctaa atacggtgcg aagagaccta aagaagctaa
    13561 aaagtaaaaa caattaatga ttgattatag ttcggtgctt taaatatata tatatttacg
    13621 gcatcacggc attaaatgat ttatgtcgag tacctatgat ttaagttaaa taattaagga
    13681 gggaagcgaa atgccaagaa aaggtaatat tccaaaaaga gaagttttac cagatcctat
    13741 gtatgggagt aaggtagtta ctaaattaat aaataatcta atggtagacg gaaaaaaagg
    13801 aaagtctcaa agaatagttt atgatgcttt cgcaattgtt gctgaaaaga caggagaaga
    13861 agctttagaa gtatttaata aagcaatgga taatataatg cctgttttag aagtaaaagc
    13921 aagaagagtt ggtggggcaa actaccaagt gccaatcgaa gttagacctg aaagaagaca
    13981 aacattaggt ttaagatggc tagtaaaata tactagagct cgtggagaaa aaggaatggt
    14041 tgaaaagcta gctaaagaga taatggacgc agctaacaac actggagctt cagttaagaa
    14101 aaaagaagat actcataaaa tggcagaagc taataaagca tttgctcatt acagatggta
    14161 ggatatatag atatcttata aaattgagga tatcataagg aggataacca tggctagaaa
    14221 gtttcctttg gaaagaacta ggaatatagg aatcatggct catatagatg caggaaaaac
    14281 tactactaca gaaagaatcc tgttttatac tgggcaaact cataaaatag gagaaactca
    14341 tgaaggagct tctcaaatgg actggatgga gcaagaaaag gaaagaggta taacaataac
    14401 ttctgccgct actactgctt catggaaaga ccatagaata aacataatag atactcctgg
    14461 acacgtggac ttcacagttg aggttgaaag atctctaaga gttcttgacg gttcagttgc
    14521 tgtattctgt gcaaaaggtg gggttgagcc tcaatcagaa aatgtatgga gacaagctga
    14581 aacttatgga gtacctagaa tagcttttgt aaataaaatg gatatattag gtgcagattt
    14641 ctacaatgtt gtaagcatga tgaaatctag attaaattct aatgcagttc ctatgcaatt
    14701 accaataggt aaagaagatt catttatagg tataatagac ttattaaaaa tggatgctgt
    14761 tatatacaaa gacgacctag gggttgaaat ggaagaaaca gatataccag aagatatgaa
    14821 agaattagct gctgagtgga gagaaaaatt agttgaatca gtagctgaaa ctgatgaaga
    14881 attaatgatg aaataccttg aaggtgaaga acttactata gatgaattaa aagttgctat
    14941 aagaaaagct actatagctt gtgaaatgaa cccagttttc tgtggtacag cttatagaaa
    15001 caaaggtgtt cagttagtaa tagatgcagt tttagattac ttaccagcgc caactgatat
    15061 accagctata aaaggtatat tagcagatgg agaagaagct gaaagacatt cttctgatga
    15121 agagccattc tctgcattag cattcaaaat aatgactgac ccattcgttg gaaaactagc
    15181 gttcttcaga gtttattctg gaacattaga atcaggttct tatgttttaa atgcaactaa
    15241 gaataagaga gaaagaattg gtcgtatatt acaaatgcat gctaatacaa gagaagaaat
    15301 aactaaagtg tatgctggag atatagcagc agcagtagga ttaaaagata ctactactgg
    15361 agacacatta tgtgaccctg ctaatccaat aatacttgaa tctatggaat tccctgagcc
    15421 agttatatct gttgctatag aaccaagttc taaagcagct caagaaaaaa tgggtatagc
    15481 tcttcaaaaa ttagctgaag aagacccaac tttcacagtt aagactgacc aagaaacagg
    15541 acaaactata atatctggta tgggtgaatt acacttagag ataatagtag atagattatt
    15601 aagagaattt aaagtagaag ctaaagttgg tgcaccacaa gttgcatata gagagactat
    15661 aactcaacca gttgatgttg aatacaagta ctctaagcaa tcaggtggta gaggacaata
    15721 cggtcacgtt aagattagag ttgctcctca agagccaggt gaaggataca aattcacaaa
    15781 caaaactgtt ggtggatcag taccaaaaga atatgttggt ccagttgaca tgggtataca
    15841 aggagctatg caatctggta tagttgctgg atatccagtt gtagacgttg ctgttgagtt
    15901 atatgatggt tcttaccatg aggttgactc ttctgagatg gcgtttaaga tggctggttc
    15961 aatggcattc aaagatgcta tgaagaaagg taatgcagtt cttttagagc cttacttcaa
    16021 agtagaagtt gttactccag aagattacat gggagatgtt atgggtgact taaactcaag
    16081 aagaggatta atccaaggta tggaagctag aagtggtgca caagttataa atgcattcgt
    16141 tccactttca gaaatgtttg gatactcaac agacctaaga tcttcaactc aaggtcgtgc
    16201 aacttacaca atgatattcg atcactatga gcaagttcca gcttcagttg ctaagaaaat
    16261 agctgaagga aaataattta aaaataatta ataatataaa tataaaaaat aatcagctag
    16321 atgagagata tctctcatct agcacaagat
//
LOCUS       DACULQ010000085        16286 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.085, whole genome shotgun sequence.
ACCESSION   DACULQ010000085 DACULQ010000000
VERSION     DACULQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.085"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            226..1221
                     /locus_tag="JA154_10795"
     CDS             226..1221
                     /locus_tag="JA154_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HAT7996350.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIDKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHRKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
     gene            1239..2927
                     /locus_tag="JA154_10800"
     CDS             1239..2927
                     /locus_tag="JA154_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HAT7996351.1"
                     /translation="MITIKNKRRISDSKIQKCFLVFLVIALVVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTNIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMSITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            2939..4006
                     /locus_tag="JA154_10805"
     CDS             2939..4006
                     /locus_tag="JA154_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT7996352.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            4198..4728
                     /locus_tag="JA154_10810"
     CDS             4198..4728
                     /locus_tag="JA154_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAT7996353.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            4899..5768
                     /locus_tag="JA154_10815"
     CDS             4899..5768
                     /locus_tag="JA154_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HAT7996354.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            5834..7099
                     /locus_tag="JA154_10820"
     CDS             5834..7099
                     /locus_tag="JA154_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996355.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEGSEE
                     EYIIEEYDEDEYEDYWEDDEDIDEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQ
                     GESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYL
                     GYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGF
                     LRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            7250..8365
                     /locus_tag="JA154_10825"
     CDS             7250..8365
                     /locus_tag="JA154_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HAT7996356.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            8551..9447
                     /locus_tag="JA154_10830"
     CDS             8551..9447
                     /locus_tag="JA154_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAT7996357.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            9517..10314
                     /locus_tag="JA154_10835"
     CDS             9517..10314
                     /locus_tag="JA154_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996358.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(10369..11721)
                     /locus_tag="JA154_10840"
     CDS             complement(10369..11721)
                     /locus_tag="JA154_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAT7996359.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            11940..12818
                     /locus_tag="JA154_10845"
     CDS             11940..12818
                     /locus_tag="JA154_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAT7996360.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            12974..13252
                     /locus_tag="JA154_10850"
     CDS             12974..13252
                     /locus_tag="JA154_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SpoVG"
                     /protein_id="HAT7996361.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            13465..14844
                     /gene="glmU"
                     /locus_tag="JA154_10855"
     CDS             13465..14844
                     /gene="glmU"
                     /locus_tag="JA154_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090036.1"
                     /note="forms a homotrimer; catalyzes the acetylation of
                     glucosamine-1-phosphate and uridylation of
                     N-acetylglucosamine-1-phosphate to produce UDP-GlcNAc;
                     function in cell wall synthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAT7996362.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            14939..15889
                     /locus_tag="JA154_10860"
     CDS             14939..15889
                     /locus_tag="JA154_10860"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090035.1"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-phosphate from D-ribose 5-phosphate and ATP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAT7996363.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
BASE COUNT         6497 a         1756 c         2952 g         5081 t
ORIGIN      
        1 tttcattatt aaagtataga tattatatat aaagtaaata aattattgag cgattattaa
       61 taagatatgt ataaatttta atttgggaac ttgtgttata tatcatgaaa aaagagaaaa
      121 aataaaaggt aaattgtttc ttgataactt gaaattggaa gtgagaatat ttaactagag
      181 aagaattatt taagtttaag tcagctttag gaggaggaat tataaatgtc ttttttacaa
      241 gtaaaaaatg taggtaaaag ttatggacaa gttaaggtgc taaaagacat atctattgat
      301 attgaaaaag gggagtttat ttgcttatta ggtccaagtg gttgtggtaa aagtacattg
      361 cttagaataa ttgcaggact tgaggataaa catgatggaa aaataataat aaatgataag
      421 gatatgacta actcaccacc tgaaagtaga aactttggta ttgttttcca atcatatgca
      481 ctatttccaa atatgaatgt atataaaaat atagcctttg gattagaaaa taaaaatata
      541 agtaaatctg atatagataa gaaggttaga gatattttag aagttgtaga attaagtgga
      601 tatgataaaa aatatccatc acaattatca ggtggacaac aacaaagggt tgcactagct
      661 cgtgcgattg ctttagagcc agactttttg cttcttgatg aacctttatc tgcattagat
      721 gcaaaagtaa gacttaaact tagggaacag attaaaagtt tacatagaaa actaggcatc
      781 acaactataa tggtaacaca tgaccaagag gaagctcttt gtttagctga taaaatggtt
      841 gttatgaata atggagaggt aatacagatt ggaactccta aagaagtcta taaaagtcca
      901 aaaacacctt ttgtagctga ttttatagga acaataaatt ttatagatga tgatgtgaat
      961 atgatagcaa ttagaccaga agatataaag gtagaattta atagagactt taaagaaatt
     1021 gatgtaaaag taggagagat tttggatata gaatttagag gattcaacta tagaattaca
     1081 gttgaatatt gttctaagca aataaagttg gacgtagttt caaaggtagt ggaacaaatg
     1141 aaactatgtg taggttctaa aataaacttc aagataccta aagaaagaac catacaatat
     1201 aaatcagagg gatgtgctta atagctagag gtggataaat gataacaata aaaaataaaa
     1261 gaagaataag tgatagtaag atacaaaaat gttttttagt ttttttagtg attgcattag
     1321 tagtatttat attatttcca ttaatatcac tattaaaaaa tgcatttatg gatacaagtg
     1381 gaagttttgt aggaatttca aactttagta aatatattga aaatcccact cttatagcat
     1441 catttaaaaa ctcaattttt gtatctacaa tttcatcctt aatatcatta tgtttagcgt
     1501 ttatatattc atatgcaata acaagaacga atataaggtt taaaaacttt tttaaaacta
     1561 tgggcatatt tccgcttttc gcaccagcta tgctatatgg tatttcactt atataccttt
     1621 ttggaaacaa gggaatcttt acaattatag gcatagaagt accactatat ggtccattag
     1681 gaataattat ttgtcaaata ctgtttacat ttccacaggc ctttttaata ttatctgtag
     1741 cactttcgat ggctgattat agactttatg aagcagcaga tagcttaggg gctagtgaat
     1801 ttaagaagtt cttcaaaata acaattcctg gtataaagta ttcattagtt agttcgttct
     1861 ttgtatcttt tatacttgca tttacagatt ttggagcagc aaagatagtt ggaggaaact
     1921 acaatgtatt ggctactgac atatataaac aagtggtagg tcaatttaac ataccaatgg
     1981 gagctactgt ctctatggta atgttaattc ctgtactaat agcatttaca gtagataaga
     2041 tagcaagtaa aaagcaaggg atgactataa ctgcaaagtc tatgccatat aagattaagg
     2101 aaaataagtt tagagataga gtatttttaa tagcatgtag ttttataact ttattaatct
     2161 ttattttgat atttgtttca gttataggct cagttataaa attatggcca tatgatttaa
     2221 gctttacaat ggaacactat acgtttaaag gtatatcagg aaatggaatc aaaacatatt
     2281 taagcagttt aaaaatatca attttaacag caatatttgg aactatattt gtatttttta
     2341 gtgcttatat gatagaaaaa gtggataagt ttcctagact gagacaaata tcttattttt
     2401 tatcaatgat accaatggca ttacctggtt tggctttagg tatatcgtat ataacttttt
     2461 tcaattcagc aagtaatcca ttaaattttt tatatggaag tacaacaata atggttatag
     2521 ccaatatagt tcatttttat tcagtttgct ttataacttc aaattcatct ttaaaaatgt
     2581 tggatagaga gtatgaatta gttgcaaaat ctataaatat tccattttat aaggtgtttt
     2641 tttatgtaac acttccaatg tccataacat caatacttga agtggctgta tattacttcg
     2701 taaattcaat ggttactgtt tcagcattaa tatttttata tatacctcaa actcaaactg
     2761 catcaatatc gatactaaag ttagaagaga tagggtatat agggccatca gctgctatgg
     2821 cagtattagt gtttttaaca aatataatag taaaactttt gtatgaattg acaacaaaga
     2881 aattaaaaac taagacacaa aaatggcaac aaaaagaagt tgcatagagg aggtaaaaat
     2941 gaaatttagg aaattagtct ctttaggaat attatcaaca cttttattga gtgtaataac
     3001 tggatgtaat aatgcttcat caggttcaaa tttaaagtct aataataaga taaaaggaga
     3061 gttaacagtt tatacagcaa ttgaagaaga tagtatagat acatatttaa ctacatttaa
     3121 agagaaatat ccagatatta aattgaatgt acttagatta tcagtaggag atataacagc
     3181 aagattatta gcagaaaaag ataatcctaa agcagatgtg ttatgggggg ttgcagctac
     3241 aagtttgata attttagatg acaaagaaat gctagagcca tacgctccga aaggctttga
     3301 taaggtaaat cctgtattta aagatgataa gaaagttcct tcttgggttg gaatagatgc
     3361 ttggatgact ggtataacag taaatactaa agaattaagt gataagaaca taccagttcc
     3421 tagttcttat gaagatttaa taaagccaga atataaaggt cttatatcta tgcctaatcc
     3481 agcttcttct ggaacaggat atttaacagt atcagaactt atacaaatta tgggtgaaga
     3541 aaaagcatgg cagtatatgg ataaattgca taaaaatatt ggtgtataca ctcagtctgg
     3601 ttcagcgcca gctatattag cagcttcagg agaatatccc attggtatat cttttggaaa
     3661 tagaggtata aagttaaaag gtgaaggtta tccagtagaa gtagtatttc caaaagaagg
     3721 ttctggatgg gagatagaat caaatgcact tgtgaaaaaa gataatataa aagaagcatc
     3781 aaaagtattt ttagattggg ctataagtaa agatgcaatg aatgagtatt ctaagaattt
     3841 tgcagttact acaattgaca cgggaaatcc ggtgccagaa ggatttccaa agaatccatt
     3901 agaacagatg atagataatg atttaaaatc agcagcaaga aatagagagg ctatactaaa
     3961 taaatggata gaaaaatatg atggaaagac agaaaaagag agctaagcct tgcaataaaa
     4021 tttagtacaa aaagcgccta gaatataagt tttatatcta ggctcttttg atatatttat
     4081 ttaaaatgca tatttctata taaaaataaa tagtttagta ccttgattaa attaaataaa
     4141 aaccttaaaa taaagagatg taaagaaaaa ctaaaaacga ataaaaggtg aatttatatg
     4201 ataaaagaaa taatagtagt agagggtaga gatgacgtta cagcagttaa gagagcaata
     4261 gatgcagaac ttataactac aggtggattt ggatttccaa aaggtgtaat ggagcgtata
     4321 aaagctgcta atgaaagacg tggtgttata atatttacag acccagattt tgcaggtgaa
     4381 aaaataagaa aaaaaatagc atcagaagtt ccaggatgta aacatgcatt tctaccgaga
     4441 gaggaagcta aaaaggatgg agatataggt attgagaatg ctactcctca aagtataata
     4501 gcagctctga ataaagttag aactgaaaat acagaaaaaa gagatgaatt taagcaagtt
     4561 gacttaatta gaaatggtct tataggtaat gaagaagcat cacttagaag agatgcactt
     4621 ggaaaaatac ttggaattgg atatggaaat gcaaaacaat ttttaaatag attgaataat
     4681 tatggagttg aaagagaaga atttatagat gcattaaaac aaatctaaac gtaatgttta
     4741 atgtatgaaa aatatgtata taaaataaat tgtggaatta cggttaattt aagatgaatt
     4801 atttataata tacaccttat tttaaatcaa tgtaaataca tataaataag tataattaat
     4861 aaaattaaat atcaaaataa gttaaaaagg agtaaataat ggatagacta tcttcacata
     4921 atgcaacaaa ggaagtagta caaaaatata attttaaatt ttcaaagtct ttaggtcaaa
     4981 atttcttgat agatagtaat ataattgata aaatcttatc tggagctaga atcacaagag
     5041 gagataatat catagaagta ggacctggta taggaactct tactcgtgag atgggtaaaa
     5101 tagcagaaaa agtagtggct atagaaatcg atagaaattt aattcctata ttgaaagata
     5161 ccttgtcaga tttagataat acagaggttg taaatcaaga tatactaaaa gtagacatac
     5221 aggaattagt aaaagataaa ttaaatggtg ggccagttaa gcttgtagca aatcttccat
     5281 attatataac tactccaata gttatgaaat ttttagagga agacatacct gtaacagaca
     5341 tagtagtcat ggttcaaaaa gaagttgcag atagaatgaa tgctattccg ggtactaaag
     5401 attatggggc attatctata gctgttcaat actattgtga tacagagatt gtggcaaaag
     5461 ctccaagaca tatgtttata ccacaaccaa atgtagattc cacagttata ggtctacatg
     5521 taagagataa aagaaaatat gatgttcata atgaagacat attttttaaa acagtaaaag
     5581 catcctttgg acaaagaaga aaaactcttc ttaattcgtt gggaggtctt ggttttctta
     5641 ataaagatga aataagagaa gtattaaaag aagctaatat agacgaaaaa agaaggggtg
     5701 aaactcttag tatagaagag ttttcagttc tttcaaatat aataaataca aaagtatctt
     5761 ctaaatagaa ggtacttttt ttaattttgt taacatatat tgtttagaga aactgtgtta
     5821 aggggggatt gcagtgggtt ctaaaagaaa gtttaaaaga gactatataa tactagaagc
     5881 taaagacata aactttagat acaaagaacg agtactgcca aaagcttttg caaaagttga
     5941 agtaaatgat gaaaaagcaa ttattgcatt atatgtagaa aatctaaaat atgttaaaga
     6001 tgggtataaa gcagtagcaa taaaagatga ccatggatta atagacttag gaaatataat
     6061 tttaaatgaa caaggaagag gtgaatttgt tttagattta gaagattatg atgatgagat
     6121 aaaaggtatt gcattattgt atgaaagaaa tataccttta attggattta aaggaaataa
     6181 gcttgaaaat tatgaggaca tattatttca agaggtagag gaagaagaat atgaagaata
     6241 tgaagagttg gaagaagaag acgaagagat agaggataat gaggaaagtg atagagacga
     6301 aggtagtgaa gaagaatata ttatagaaga atatgatgaa gatgagtatg aagactattg
     6361 ggaagatgat gaagatattg acgaaggaga gttaaaagca agaacggaag tagaagattc
     6421 tggtgatgag gaaatagaag agttcaataa ttataaggaa atagaaaatt ataatagggt
     6481 taaagtacag gaagaatata tagacataga atatgatgag gaagaagagg aagaagaaga
     6541 agtttttgaa gtaaatagag aagaagatag gtttgtttcc tcaaaaccta ctaaatcaaa
     6601 gaaagttcaa aaagatcaga ataatattga aaaaataaaa agagctaatg gagtaagtca
     6661 aggagagagt ggaaataaac aaaccagtac attacttatg ccaagacaaa taaaaaaagg
     6721 tttgaaatta tttaaagaag taaaaccttt tgtaacagat tatatagata agacaagatg
     6781 gtggaaaata gagataaatc ctacaacatt gtgtggatat acaatgcctt atttaggata
     6841 tttaaacgtg ttgaattaca ccatgtatag tgatgcagtt ttacattcat ataaatacag
     6901 acactattta tttggagtcc agtatgatga atataataag agaaaacatt atatttatgc
     6961 tatcccagga aatagaaatg aacagccaga caatggaagc acaggatttt taagatatca
     7021 agcttgtgat aatagaacaa atagtttagg atattggcta tgttttatag attctagaca
     7081 aagaaatata gttaggtaaa aataataaaa atgaaaaaat ttagactcaa ttcttatttt
     7141 actgaaaaaa ttaatgaaat gtagtacaaa actttaaaaa ctattgataa tatttaaata
     7201 ttacactaca atatatcttg aaatacctat tatattatgg aggaaaatta tgataaacga
     7261 gcaaagaatt ttaaatgagt ttcttgaatt agtacagata gatagtttat ctttaaaaga
     7321 ggggaatgtt gctaaagtat tagttaaaaa attagaagaa ataggttgtt cagtagtaat
     7381 agacaatgct ggtgaaaaag caaatggaga aactggtaat ataatagcta cattaaaagg
     7441 aaataaagaa ggaaagaaaa tattatttag ttcacatatg gatacagtaa ctccaggtat
     7501 aggggtaaaa cctatagtgg atgaagcaaa tggaataata aaaagtgatg gtactacaat
     7561 attaggttct gatgataaag caggtatagc agctatatta gaaggcttaa gatatataaa
     7621 tgaaaataat atagaacata ctgatataca agttgtattc tctatatgtg aggaatgtgg
     7681 attagttgga gctaaaaatt tagactatgg aaaaatagat tctgaatatg catttatatt
     7741 agatagtggt ggttctccag gtgaaataat agtaaaagct cctgcacaag atgtaataaa
     7801 tgttaagata ttaggaaaga ctgctcatgc aggtcttgaa cctgaagctg gaataagtgc
     7861 tataatggta gctgccagag caatagaaaa tatgaattta cttagaatag acgaagaaac
     7921 aactgctaac ataggaatta taaacggtgg aacagctaca aatatagtaa caggagaagt
     7981 taatataatt gcagaagcca gaagtctaaa agaaaataaa ttagatgttc aaactaaaca
     8041 catggtagag acttttgaaa aagctgcaaa agattttgga gctcaaattg agatagatgt
     8101 caatagagca tatacaccaa tagatgttag tgaggattct gaaataataa aacttgcaaa
     8161 gaaggctttt tcaaacttag gtatagaagg tcatactgaa tctacaggag gaggtagtga
     8221 tacaaatata ttaagtaaga atggtattga agctataact ttaggaattg gtatgaaaaa
     8281 tgctcataca ctaagtgaac atatagctat aaaagactta tatgattctg ctaaaatggt
     8341 agtggagata ataaaggaag cataaattta aataatagtg tataatatat atatcgccta
     8401 gcttaagcta ggtgattttt cattttatag aagattgata atctctattg aaagtaaaga
     8461 gtatacattt gcatataaga gagtgtttaa tttatgttca aatgtaggtt cttaataata
     8521 tttagattgt ttcataaggg aggaataaat atggaaacaa ggtatgaaga agcaaataaa
     8581 ataacaatac aatcaatact ttggaatgta gtattaacaa taattaaggt tatagctggt
     8641 gttattggta attctagcgc tatgattgcg gatggattac attcagcatc agatattata
     8701 agttctatag gagtactaat aggcaattat gtatcttcta gacctggaga tagagagcat
     8761 aattatggtc atgagaaagc agagacatta gtatcatttg tgttatcaat attgcttatt
     8821 ttcgtttcta taacaatagg tatagaggca attaaatctt tatttaactt agatgcatta
     8881 agtgttccat caatattacc attagtagta tcagttattt caatcctgat aaaagagtat
     8941 caatatagaa taacaataaa agttgctaaa aagataaatt caccagccct aaaagctgat
     9001 gcttggcatc ataggtcaga tgctttatct tcagtagctg cttttatagg tataggtggt
     9061 tctattttag ggtttaaacc attagaccca attgcgtcag ttgtagtggc tatatttgta
     9121 gctaaagtag gaattagtat acttataagt tctgttaatg aattgatgga tgtttcagtt
     9181 gatgaagaag agataaaaga attaaaattt attgtagcag atacagaagg tgttaaaaat
     9241 cttggtgata taaagactag aaaacatgga gctatggcat atgtagattt gactatatgt
     9301 gtggatgaaa atttgacagt taagcaaggt catgatattg ctaccaagct tgaaaaacat
     9361 ataataaaac atatggaatt tgtaaaaggc ataacagttc acgttgaacc atgtactaat
     9421 tgccaaggaa ataagtgtaa taattaatta aaacttaaaa aaaagtgtaa aaagttatat
     9481 aattgtttta tgattataaa ctctaggggt ggttaaatga gcaagaaaag aaaactaaag
     9541 aaaaaagctg caatattaat aggagtagtt gttttcttta taatatactt tatatccttt
     9601 agaataggaa taaatatgga aaaggataaa agtgaagtgc caaagaaggt agaaaaagaa
     9661 gaagaagata ataggtcttt aaggactcaa ataaaagaga agaagaaggt ttatatatca
     9721 gataaaaata tagaaaacat aaaaattgaa gaagatatgt gggatgaaat aaagttattt
     9781 ttctcagatt ttaagaaagt aagaaatact acttcttctt acgaagcagt ttatgaaggt
     9841 tattctgatg atggagttaa attttctact gaccttaact tttttagagt atatactgta
     9901 aataaagaag aatattataa agtaccagtt agttctaagg atgcttttaa gaaactttta
     9961 gatgagagca tatatacttc gtttgacctt ttaagtcatt ataaagattg gaaagaggtt
    10021 actataactc atggaaaaga aattaaaaaa gttcatagat ggaaatttga tgatttagca
    10081 cataagatga tttcaaagag ggttgtagga aaagttcaac ctgagaaaag taaggaaaga
    10141 agtgactata attttactat aaatataaaa ggtgataatt ttgatgtaaa agttgataca
    10201 atgggaaaag attatgtaaa agttacttct aagaaaaatg tatcttacta tgaggttcat
    10261 acaagtttat ttgattacct taaaaatgag atatttgaaa ttggaaagaa ataagacttt
    10321 aagaaggcta tcaaattaga tatttgatag cctaaattat tttatatatt atttttcaac
    10381 caacaagtct gcaaccttat aaattggacc agcaccagca gttattacta aatcattatc
    10441 cttaacattt tcacgtaagt attttactat atcttcaaat tcttttatat agatggcatc
    10501 tacattgttt tgatataatt tctcaaccaa gtccttagaa tgtatatctc ctgggtcttt
    10561 ttccctagca gcatatatat ctgttatgat aactttatca gcagcataga atgcctctga
    10621 aaattcacct aaaagagatt ttgttctagt ataagtatgt ggctggaaga tacaccataa
    10681 agttgatttt tttaattttt tggcagcagc taaagttgct tttaactcag ttggatgatg
    10741 tgcataatca tctactacta aagcattctt ataataccct ttagtttcaa atcttcttcc
    10801 gacaccttta taaattttta tattttttct tatagtctct aagtcaatac cagaaacaag
    10861 agctgccatt atggcagaag aagcattgta tatgttatga agaccatata cagaaagttc
    10921 aaattctcct aaatctcttc cttcatattc tattctaaat attccatgac cactattgtc
    10981 aaagtgaata tcttttatta cagcatcatt gtcagaatct ctgccgtatt ttataatagt
    11041 agctttaaca tcatacaata tgtcatcaat attttcatca tcgccattta ttatgaaata
    11101 gccttctggg ggtaataatt ttccaaattt gttaaaagaa gctttgattt catctattcc
    11161 agagaaataa tcaagatggt cttcttcaac atttaaaact atagatatct ttgggttaaa
    11221 attaaggaaa ctatctacat attcacatgc ttcagttata aagtggttgg aattacctat
    11281 cttaacatta ccacctatca tacttaagtt accaccaact agtatagttg ggtctaagtc
    11341 tgcatattca aagattgtag ataacataga tgttgtagaa gtttttccat gtgttccaga
    11401 aactgctata gaatttttat attccctcat gatttggcct aaaaaagtcg ctctgttcat
    11461 taccaattta tttttttctt tagcagccac aagttcttca ttgtctggat gaactgctgc
    11521 tgtataaaca accatattta catcatctga tatattatct tttttctgac caatataaat
    11581 agttgcccct tggtccctaa gtttgtctaa taggtatgat tcgtttgaat ccgaacctga
    11641 aacttgataa cctttattga tacatatttc agctaatgca ctcatgctga taccgccaat
    11701 tcctataaag tgtatattca taataaaacc acctttcttt ttaattaagt cctacaatat
    11761 gaactatttc tgtggtataa taatataatg ttcaatattt tataattaat atgcatataa
    11821 tattatatgt attttatttt tgaatataat aaaacataag taatattata ccacaataag
    11881 aattgttatg cattagttag tttaacgtta tataaaggta cttgtaggag gaaataataa
    11941 tgaaatttaa aagaacagag agaattggag caatagttaa aatactgtct gataatccaa
    12001 ataaaatata tactttaagt tattttacaa atcaatttaa tgcagcaaaa tcaacaataa
    12061 gtgaagattt attagttgta aaaaatgtat ttgagaagct acatttaggt aaagttataa
    12121 ctatttcagg agcagcagga ggagtaaaat atatacctaa aacttctata gcagaaaatg
    12181 cagagttctt aatggaatta tgcgagagaa tatgtgacaa gtctagaata ctatctggtg
    12241 gttttttata cttgattgac ctaatttacg accctacaat agctgctaag ataggaaaga
    12301 tattcgcatc aaatattgag tatgtagatg cagattatgt tgtgacaatg gaaacaaaag
    12361 gtatacctat ggcacttatg actgcaaaag ctatgaattt acctctagtt ataataagaa
    12421 aagatataaa ggtatcagaa ggtcctacac ttagcatgac atatgtgagt ggaaacagtt
    12481 ctaaagttga aagtatgagt ttgccaagaa aagctcttaa accagatagt aaagttataa
    12541 ttatagatga tttcatgagg ggtggaggta ctataaaagg aatggtagac cttatgaatg
    12601 aatttggtgc agaggttata ggtactggag tattcatatc tacaacgaat ccttcagaaa
    12661 aaatggtaaa agattatatt tcgttgattc aattggatgt taatggagat aaaatagtag
    12721 tagaaccaaa cttaaagact tttaaagatg aatatagaaa tgaagattta gacgaagaag
    12781 aagattcaga atttgaattt gagatagatg aagattaata aaaaaaatat ttagaaaaat
    12841 atgtaaaaat aaaaagagga tatccctagt tgttcataga attatttaaa aaacagttta
    12901 tattaattta ttccagcgat aataatttta atataaatta atttctgtca aaatcaaagg
    12961 gggatatttt ggtatgaaga taactgacgt aagagtaaga aaattaacag aagaaggtaa
    13021 gatgaaatgt atagtttcaa taactttcga taatttgttt gtagtgcatg atataaaagt
    13081 tatagaaggc cataatgggc tatttatagc aatgcctagc agaaaagtag gcgaaggaaa
    13141 tttcagagat atagctcatc caataaatgc agaaatgaga caagttttag aagatgcagt
    13201 tttacaagcg tatcatgaag cattagttca atgggaagta gctgctgaat aataataaat
    13261 atataaaatg gagagagccc gaacatttag gctcttttta aatgtagaat ttctattaaa
    13321 atatgtactt tttagtaaaa aagtgctata ttattagtga taatgtatat gttgtataca
    13381 aattgttaga taaatgtatt ataatgtata ttgattaaga gaataatacg aataaaatta
    13441 aattagattg aatggagtgt atcaatgaac tttaaagcta taatccttgc tgctggcaaa
    13501 ggaacaagaa tgaagtctaa atacccaaaa gttatacata aagtatgtgg aaaagaaatg
    13561 gttaaccata ttatagatgt ttcaaaaaaa tcaggggtaa aagatactgt cgtgatttta
    13621 ggacatgaag ctgatgtagt taaagaaaag cttgcagaag aaattataat agccatgcaa
    13681 acagaacaac ttgggacagg acatgctgta aagatggcta aagaatatat taatgatgaa
    13741 gatacaatag ttgtactttg tggagatact ccacttataa aagaagagac actaaagaga
    13801 ctatttgaat atcatataga aaataaatat catgctacag ttcttacaac aagagttgga
    13861 aatccaacag gttatggtag aataataaga gataagaagg gtgaccttct taaaatagtg
    13921 gaacaaaaag atgcaaattc agaagaaaag atgataagtg agataaactc tggaatttat
    13981 tgttttaatg gaaagagtct tagagaagca ctagatttgc taaataataa caattcacaa
    14041 ggtgaatatt atttaactga tactgctaaa ataatgagag ataaaggatt aaaagttggt
    14101 gcatttgcag gttcaactat tgaagaactt atgggcgtta actcaagagt agaattatca
    14161 aaggcagaag aaattatgag acgaagaata aatgaatctc atatggtaaa tggagtaact
    14221 ataatagaca ctaactctac ctatatagaa tctgatgtta tgataggcaa tgatactata
    14281 atatatccag gtgttatgtt acaaggaaaa acaaggatag gttcagattg tataattgga
    14341 atgaattcat ctataacaaa ttcagaaata ggtgatggta cagaaataaa aaattcaact
    14401 ataattgata gtaaggttgg agaaaattct actgttgggc catatgcata cttaagacca
    14461 aagagtgact taggaaataa tgtaaaaata ggcgattttg tagaagtaaa aaatgctatt
    14521 atagaagatg gctcaaaagc atctcacctt tcttatatag gagatgcaca tgtcggtaaa
    14581 aatgtaaata ttggatgtgg agtagtattt gtaaactatg atggtaaaaa taaatttaaa
    14641 tcaatagtaa aagacaatgc gtttataggt tcaaattcta atttagttgc accagtagtt
    14701 gtagaagaaa aaggatatat agcaacaggt tcaacaataa cacatgatgt cccagatggt
    14761 gctttggcaa tagcaagaga aagacaagtt atcaaagaag gatgggtaga aaaaaagaat
    14821 caaaaagacg accaaagtaa gtaattatgt agtttattat tttggctaaa aaatttagct
    14881 cgaaattatt ataaattata ttcattagta ttttaaaatt ttcaggaggg ttacataaat
    14941 gaatactagc ggaagcgaga ttaaaattat tgcaggtaat tcatcgaaag agttagcaca
    15001 aaaaatagct gactatatag gtgtatcagt actagattgt gaagtaggta catttagtga
    15061 tggtgaaata tgtgtaaaca tgaatgagac agtaagaggt tgtgatgtgt ttgtagtgca
    15121 atctactaac agcccagtaa atgataactt aatggagtta ttaatcttaa ttgatgcgtt
    15181 aaagagagca tcagcaggta gaataacagc agttattcct tactatggat atgcaagaca
    15241 agacagaaag gctaaggcaa gagatccaat cactgctaaa ttagttgcaa atttaataac
    15301 tgctgctgga gcagatagag tattaacaat ggatttacat gcagctcaaa tacaaggata
    15361 ttttgatata ccattagacc acttactagg tggaacaata ttagcaaatt attttaatga
    15421 aaagaaaata gaagatttag tagtagtatc tcctgactta ggaagtgtta ctagatcaag
    15481 aaaatttgct aatactttaa atggagaagt tccaatagct ataatagata aaagaagacc
    15541 gaaagctaat gtatgcgaag ttatgaactt aataggtgat gttaaaggta aaaatgttat
    15601 actattagac gatatgatag atacagcagg aacaatagtt aatgctgcaa atgcacttaa
    15661 agagtttgga gcaaaagatg tttatgcttg ttgtactcat ggtgtgttat caggtccagc
    15721 tattgaaaga attgctaatt cagaaataag tgagttaata gttcttgata ctatacaact
    15781 tcctgaagag aaaagaatag ataagataaa gataaaaaca gtagctcctt tatttggaga
    15841 tgctataaga atgatattct ctaatgaatc agtaagtaaa ttattctaat ttattgtatt
    15901 tatgagagtt gtttaaaagg atataaacct tttgagcaac tcttttttga ttttataatt
    15961 atggaatgtt atttttttct tgttagaaag gtatatatta ttgtaaaaag agattatagt
    16021 ttaaaattat gtatatttaa gtaaaataaa cttaataaag ggcatattat tcaaaatatt
    16081 atgaatataa aaaatattac aaaaaagtat tgaaaaaatt cagattatgt agtattatgt
    16141 aaatataaat aaagaaagaa agaggtgttt ccaatgggaa ggttagaaga tgaaccccta
    16201 ataattaaat gattcagtca caatctaaag tgaatataaa aatctattga aagtttattt
    16261 ataagaattg aaaatagtat ctattc
//
LOCUS       DACULQ010000086        16193 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.086, whole genome shotgun sequence.
ACCESSION   DACULQ010000086 DACULQ010000000
VERSION     DACULQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16193
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.086"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            178..1011
                     /locus_tag="JA154_10865"
     CDS             178..1011
                     /locus_tag="JA154_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HAT7996364.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1080..2036
                     /locus_tag="JA154_10870"
     CDS             1080..2036
                     /locus_tag="JA154_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl isomerase"
                     /protein_id="HAT7996365.1"
                     /translation="MNKKLYIGMVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVL
                     SATNNYINGQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVD
                     SKYQELEDKMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDM
                     EAYYTSHKKDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESF
                     ESLAKKYSDDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVK
                     VTDKRERQKSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2088..2570
                     /locus_tag="JA154_10875"
     CDS             2088..2570
                     /locus_tag="JA154_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HAT7996366.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2582..3451
                     /locus_tag="JA154_10880"
     CDS             2582..3451
                     /locus_tag="JA154_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-V factor rsiV"
                     /protein_id="HAT7996367.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3494..4249
                     /locus_tag="JA154_10885"
     CDS             3494..4249
                     /locus_tag="JA154_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HAT7996368.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4291..5112
                     /locus_tag="JA154_10890"
     CDS             4291..5112
                     /locus_tag="JA154_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HAT7996369.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5117..5878
                     /locus_tag="JA154_10895"
     CDS             5117..5878
                     /locus_tag="JA154_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HAT7996370.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLA
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6039..6296
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6409..6648
                     /locus_tag="JA154_10900"
     CDS             6409..6648
                     /locus_tag="JA154_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996371.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            6958..7947
                     /locus_tag="JA154_10905"
     CDS             6958..7947
                     /locus_tag="JA154_10905"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088065.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAT7996372.1"
                     /translation="MARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVHVV
                     IGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKEGQ
                     TLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYTKK
                     ATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVDAG
                     YQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQGMQ
                     NVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            7994..9688
                     /locus_tag="JA154_10910"
     CDS             7994..9688
                     /locus_tag="JA154_10910"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAT7996373.1"
                     /translation="MRMSGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFK
                     HIRTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQ
                     VPTSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVD
                     VPKDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPII
                     YAGGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLA
                     LSNSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLV
                     LSLLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVT
                     DVGQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSF
                     RMNCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVN
                     AYNIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            9899..10576
                     /locus_tag="JA154_10915"
     CDS             9899..10576
                     /locus_tag="JA154_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAT7996374.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            10750..12786
                     /locus_tag="JA154_10920"
     CDS             10750..12786
                     /locus_tag="JA154_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996375.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(12977..13543)
                     /locus_tag="JA154_10925"
     CDS             complement(12977..13543)
                     /locus_tag="JA154_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7996376.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            13831..14766
                     /locus_tag="JA154_10930"
     CDS             13831..14766
                     /locus_tag="JA154_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7996377.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            15231..>16193
                     /locus_tag="JA154_10935"
     CDS             15231..>16193
                     /locus_tag="JA154_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7996378.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETMNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKE"
BASE COUNT         6680 a         1582 c         2846 g         5085 t
ORIGIN      
        1 attctataca attatacaga aattatacag cataataatc accgaaataa caaattttaa
       61 aaattaagca aaactttttg tcgaagttca acgtctatat atatgtagtg taaatttttg
      121 ggataaaata ctacaaaaat ataaatgtaa aaatatagta atattggagg tcgaacgatg
      181 agaaataaaa agaaaaaaat aaatagaaag aagttgtatt tgcttttaag tgctattgta
      241 gtgtgtctgg cttttatagt agttggtgtt cgcgtcagct ctttaaatat gaaggaaaat
      301 gaaattagta gaaatgcaaa actgtcaagt tctactatta cagatatagt atctaataca
      361 tcaatagaag ttagtaaagg gccaagtaaa agttctggaa aaatagcata cataactata
      421 gatgatggtc catctaaatt tactgacgag atgataaaaa ctttaaataa atacaatgtt
      481 aaagcaactt tttttatgat agatgggaat atgaaggaat atccacagca agttaaaaat
      541 ataattaaaa atgggaacac agcaggtttt catagtgtat cacatgatat tcacaagctt
      601 tatgttacta gtacatcagc aaaagaggaa tttgatacaa atgatcaaac tttttataag
      661 ataactggta aacattcaaa agtaataaga ataccttatg gaagcaaacc atatacacca
      721 caagcctctt atcaggcttt agttgatgct ggatataaaa tttgggactg ggacttagat
      781 acagaagact ggagatctaa ttcttctcaa attgtacaaa atgtaaaaaa tcatattaag
      841 aatagaaaag gtgaagataa agaccaactt gtagttttaa tgcatgagaa aaaacagagt
      901 gcagaagctt tagattcagt tttaaaattc ttatcagatg aaggttatga atttgcgcca
      961 gtagatcaaa accaaatacc aaaaaattat tggttacgta atttagagta gttaaaatat
     1021 aagaatattg attgatattt ttagaaattg agaataaaaa tgagtgaggt gtaggtatat
     1081 tgaataaaaa actttatata ggaatggtcg gaatattgtc tttgatgatg gttggttgta
     1141 ataaaagttt agcaaaagta aatgatgtgg aaatcacaaa agaacagtac aaaaaaacaa
     1201 aagcagtttt atctgctact aataattata taaatgggca gtctttagat gaattagaaa
     1261 aaactttgga taaaaaaggt agaaataaat tagaaaatgt tataatatcg tttatggtgg
     1321 ataatgaact tttatatcaa gaggctaagg ataaaggact aactccaagt aaaagtgaag
     1381 tagactcaaa atatcaagaa ctagaagata agatgaattt gaatacaagc tataaggaaa
     1441 aaatggacaa agcaggtgtt gataaagatt atttaaaaca agaaatatct agagatttag
     1501 caatagataa aaataaaaag gcctttgaag atagaataaa cataagtgat aatgatatgg
     1561 aagcctacta tacaagtcat aaaaaagatt ttaatgtaga agaagtaagt gcttctcaga
     1621 tacttatatc gactttagat aaaaataaaa aagaagtaag caaagataaa aaagaagctt
     1681 tgaagaagaa ggcagataat atattgacta aaatcaaaaa tggagaaagt tttgaaagtt
     1741 tggctaaaaa atattcggat gataaagcaa ctggaaaaaa tggaggacag ctaggatatt
     1801 ttacaaagga tgataaaaat gctgagttta caaaggaagt gttcaaatta aagaaaaatg
     1861 aagtttcaaa tgtgtttgaa actagttatg gatatcatat agtcaaagtt actgataaaa
     1921 gagaaagaca aaaaagtttt aatgagtgtc agagtttaat aagagaatct attttaaatg
     1981 aaaagtatat tgagcatata aaaaaactga atgaagatgc taaaatagac agatagaatt
     2041 tctaacagct caatcctagc ctgtaatcgg agggagattt tacaggaatg tttaagagtt
     2101 taaagagaac gaaagttgag aaatacatta tcgataataa agactctttt tatagaatag
     2161 catatagtta tacgaagaat gaagaagatg ccttagatgt ggttcaagaa gccatgtata
     2221 aggcactata ctcagtggaa aatattaaag aagtaaatta tataaagaca tggttttata
     2281 aaattttagt tagaacgagt atagatttta ttagaaaaaa ccgtaaatat aataatatga
     2341 cagatattga ccttatagat gagactggag agtatgataa atatactgat ttggatttaa
     2401 gaagagcttt ggaagaatta cctattgaat acaaatctat tataatatta agattttttg
     2461 aagatttaaa gattgaagaa gtagcaataa tattggatga aaatgtgaat actgttaaaa
     2521 ctaggttata tactgcttta aaaaaactaa aattaaaaat tgaagagtag ggataaaaag
     2581 aatgacaagc aaagaaaggt taaataagtt aaaagatgag tatcataaaa ttccaatacc
     2641 caagaaatta gatactataa taaatcatga aaaaatagaa aacatatatc gagagaaaaa
     2701 taaaaaagta aatagattaa gatttaaagt tgcaatagcc tttgcatgta tatttacagt
     2761 attagtaaat ataagtcctg tatttgcaga caatttttct aagatacctg taattggagc
     2821 aatagtagaa gtaataacta taaaaaatta tagtttaaaa agtgaaaatt atgaagctga
     2881 gattgatatt cctaaaatac gaggtttaaa agataaaaac ttagaacaaa gattaaacag
     2941 tagttttatg gaagatggaa aaaggcttta tcatcaattc caagagagaa tggagaagat
     3001 acaatcatca aagaataaag gatataaatc tttaagtttg agttatagtg taaaaaataa
     3061 ttctaaaaaa tttctatcaa tagagatgac aaaaaatgag atagaggctt catcatatgt
     3121 gagtaaggta cattatacta ttgataaaaa aagacaaata gttttgactt tgccaatgct
     3181 ttttaaagat gataagtata taaaagttat aagtgataat ataaaagaac aaatgagaga
     3241 acaaatgaaa aaggatagta caaaatccta ttttatagat caaaaaaaag acttgcctgt
     3301 tgaagatttt aaaacaatta ataagtatca ggatttttat tttaataaaa atgaagacct
     3361 tgtaatttgt tttgatgaat atgaagtagc acctggttat atgggcgcag ttgagtttgt
     3421 aataccatat aaagtaataa aagatttgta aattgaatag tagtttatat ataaatcttt
     3481 taaggaggac aatatgacta catataaaaa acttaaaaaa tctatattta aaattgctat
     3541 attttcaata tgcttattag tttcattagt agatacaaac cctatatttg caggtagttc
     3601 ctttaatgaa acttcaaata gtgatacgat aaaggtgtta aatgctaaga gtatcaactt
     3661 taaacaagac aattatgaag tgaatgtgag tattcctaaa ataaaaggtt taaaagataa
     3721 aactttagag aataaattaa acaatgagtt tttgcaagat gggaaaaaaa tttacagtga
     3781 atttcaaaat aaaatgaaaa aggaaaataa taagggtcat agatacctaa atgtaatcca
     3841 taatgtcaaa aataattcta aggacttttt atcaatagaa atgacaaaag aagagataga
     3901 agcttcctca tctataacta agaagcatta cactgttgat aaaaatagag gaatactatt
     3961 aaatttacca atatttttta aagatagtag atatattaga gctataagtg ataatataaa
     4021 agagcagatg agaaatcaaa ttaagaaaga tgatacaaaa tcttatttta tagaccaaga
     4081 taaagactcg ccagtgaaag attttgaatc tataaataag tatcaagatt tttattttaa
     4141 taaaaatgga aatcttgtga ttagttttga tgaatatgaa gtagctcctg gttatatggg
     4201 agcagtagaa tttgtgatac caaataaagt aataaaaaga ctaagataga tatatagaag
     4261 atttttagat agactggtaa ggagaattat atgaatatat atgaacaagc aataagatta
     4321 atagaaaata atgaggattt cgcatttgct acaataacgt ctcattcagg ttcaacacca
     4381 cgtgaaactg gagcaatgat gattgtgaaa aatgattcta ctatatttgg ctcagtagga
     4441 ggaggtagtg tagaagcaga atgtataaaa catgcaatta atgttataaa aaacagagaa
     4501 tcaatgttgt ataaatttac tctaaacaaa tctgatgtgg caaaactagg aatgatatgt
     4561 ggaggtactg gagaaataca aatagatttt atagatagta aattaaaaag taatatagaa
     4621 aaatttaata agagactaaa ggaaaataca agtaaagcat atatatttgg tgcaggtcat
     4681 atttcaagag atgttgctgt gatactatct cttttagaat ttagaacagt agtaatagat
     4741 gatagagaag aatttgctaa tcatgaaagg tttccagaat cagaagtagt agttttagat
     4801 tcttttgaaa acataccaga ttttcccact gacgaaaata gttatataat tattttaaca
     4861 agaggtcatc tatatgattc aagtgcttta gaatgggctc ttaaaagaga ggctggttat
     4921 ataggtatga ttggaagtag aacaaaaatt ggattgactt atgaaaaact catgaaaaaa
     4981 ggttttaaga aggaagaatt atcaaaagta catgcaccaa taggcataaa attgaatgct
     5041 caaacaccag ctgaaatagc tgtatgtata gctgctgaac ttataaattg tagagctaat
     5101 aaggaaaaat aaactaatga acacagggag taaagatatt gatactttaa aggttaattt
     5161 tagagggatt gaaataaaag cagttttagt atataaaaat agaaaaaata tttctataaa
     5221 aatagatcca tttggcaaaa taactgtaat gagtcctcct aaaatatcca aaaaaataat
     5281 aaaagatata attattgaaa aaggagactg gatacttaaa aagtcagaaa aatgtaaagg
     5341 aagagaggaa gtatataaac aaagaatgtt tataactggc gaaaaatttc tttatttagg
     5401 tgaagagtac catctagtta taaaagaact gttgagagat tctgtcaaaa aaagtaactg
     5461 tagaataact attaacgaat atcaaatagt tgttcagaca aatgatacaa gtacagattt
     5521 tattaaaaaa tctttaaaat cttggtataa gatggaaagt gaaagaatag tattagagag
     5581 aatagatttt cttaaattaa attgtgaaat aatgaagcaa ttagttccag cgagtgttaa
     5641 ggtaaaagaa caaaataaga gatggggaag ttgtacagct caaaaaaata tttatataaa
     5701 ctcaaaaata tctatggcaa gattagatgt aattgattat gtaataatac atgagtttag
     5761 ccatttagca catatgaatc actctaaaaa attctatgat ttagtaaaat ccattatgcc
     5821 cgattataaa gataaagaaa attggctaaa aataaatgga tataaaataa taatctgaaa
     5881 acgattattc caaaatatag gcgtaaagta taaaagaagt attattgtga aaaaaataaa
     5941 taatctgaaa attttaaaaa aagtcattga cattgtaaat gatagagaat ataatctata
     6001 ttaacaacaa aacagaattg aaatataaaa ttaaaaaatt aaaagcaatg acagagacaa
     6061 caatactaat agtgttatcc acagagaacc aatgttgctg agaattggtg ttaacagtag
     6121 tataatgatc actctggagc ttttgtactg aatattttta gtaagtacaa acgtatgtca
     6181 acgttacatg actaaggtta aggtaattaa ttacccgacc tgaaaaaaga ggggtagatg
     6241 tattttttat atctacaaat aaggtggtac cgcggaatat aactttcgtc cttataagta
     6301 atatttatat tgcttataaa gatgaaagtt tttttatttt aagtgatata aataaattta
     6361 atccatataa aattaataaa aaaataataa aaataaggag ggatttttat ggaaaatact
     6421 gtatatgcta aattaaacca aggaatagat tcttttttaa gagtggctat gactctaaga
     6481 agaagagagg ttgatataca atcaataagt atgactgtag ataatatgaa taattcaggt
     6541 ataaaattaa tagtaaatga agaaaagaca tccttagatt ctgtcttaaa ccatatgaaa
     6601 aaactacatg atataagaga gataactgtt gaaagaatga gacattaatt aaatatattt
     6661 tataaaacta aagataaata tgcttatttt aaaataaaaa actaaagata aatattaaat
     6721 aagaaaaatc ttagattaaa atataagtgt cattgaattt attagtttat cacagttaat
     6781 aaatttatta gtttgttgta gctaataaaa actgaaaatt ctaaaattat aattgtaaga
     6841 ttatataaat ttagaattta aatctgttga ataatattaa aacaatgata atgagtatga
     6901 atttctaata aaaataaata tcataaaaat aagaaaaagt attgggagga aatgaaaatg
     6961 gcaagaatgt attatgaaaa agatgtggat ttagaggttt taaaaaataa aaaggttgca
     7021 gttttaggtt atggaagtca aggacatgca catgcacaaa atcttagaga taacggtgta
     7081 catgtagtga taggtcttta tgacggaagt aaatcagcac aaaaagcaaa agaagatgga
     7141 tttgaagtta agagtgtagc agaagctaca aaagaaagtg atttgacaat gatgttaatg
     7201 cctgatgaaa agcaaaagaa agtttatgaa gagagtgtaa aggataactt aaaagaaggt
     7261 cagacattag cttttgctca tggattcaat atacattata atcaagtaca accaccagaa
     7321 tttgttgatg ttgtaatggt agctcctaaa ggacctggtc acttagttag aaatgtattt
     7381 acaaagggaa gtggagttcc tgcactattt gcagtatatc aagactacac taaaaaagct
     7441 acagagacag ttttagcata tgcaaaaggg ataggagcaa caagagcagg tgttttagaa
     7501 actactttta aagaagaaac agaaacagat ttatttggag aacaatcagt actttgtggt
     7561 ggaataagtg aacttataaa attgggatat aaaacgcttg tagatgcagg ttatcaaaaa
     7621 gaagtagcat attttgaatg tttacatgaa atgaaactaa tagtcgacct tatatatgaa
     7681 ggtggatttg aaagaatgag atatagcata agtgacacag cagaatatgg agattatgta
     7741 tctggtaaaa gggtaataac tgatgctgca aaacaaggta tgcaaaatgt tttagaggat
     7801 atacaaaatg gaaaatttgc aaaagcgtgg attaaagaaa atgaagaagg acgtgaaaac
     7861 ttcctaaaaa ctagagaaga agaatataat acagaaattg ccgaagttgg tagaaatcta
     7921 agaagtatga tgtcattctt aaaataaata tatattaaat ggattagttt gagggaaaga
     7981 gtggtgtata gtagtgcgta tgagtggagc aaaggtaatt ttagaatgtt taaagaaaga
     8041 gggaattgac acaatatttg gatatcctgg tggagccgtg atacctcttt atgatgctct
     8101 atacgattat tcagatgatt ttaaacatat aaggacatcc catgaacaag gtttagttca
     8161 tgctgcagat gggtacgcaa gaagtactaa tacagtagga gtttgtttta caacatctgg
     8221 tccaggagca acaaatgcca taactggaat agcaacagct tttatggatt cttcacctat
     8281 ggttgtaatt tcagggcagg tgccaactag tttattagga aaagattctt ttcaagaaat
     8341 tgacataact ggagcaacat tatctatgac aaaacataat tatttagtta gaaacactaa
     8401 agaattagtt cctacaataa aagaagcttt tagagttgca aattcaggta gaaaaggacc
     8461 tgtacttgta gatgttccaa aagatttatt tttagcagaa atggatttta gtggtgagga
     8521 ctatgattta tgtcaaatag atgattatat ggattataag agtgattttg atctagatga
     8581 tgaaactaat attaagcttt taaatgaagc aatagatatc ataaaagaat ctaaaaagcc
     8641 tataatatat gcaggtggag gtgttaaatc ttcagacagt gaggaaattc ttgaaaaatt
     8701 tgcaactaag atagatacac ccgtactaaa tacacttatg gggcttggaa atatagatag
     8761 aaagaatgaa ttatctcttg gaatggttgg aatgcatgga agtagagaaa gtaatttagc
     8821 actttctaat tcagatttag taatagcgat aggtgcgaga tttagtgata gagtgataag
     8881 taaaagttct gaatttgcta aaaatgcaaa aataatacac atagacatag acccatctga
     8941 aataagtaaa aatatagaat ctaatgtatc tttggttgga gatgtaaagc tagtccttag
     9001 tttattaatt gaaagggttg aaagtaagaa taatagcaat tggaaagaag agataaagag
     9061 atttagaaag agtgagggtg tacaaacata tgaatttcat ccacaaaata tactaaaaaa
     9121 aataaatgaa aaatatgaaa cattaaagaa acccactgtg gtagtaactg atgtcggtca
     9181 acatcaaatg tgggctgcta agtactggaa ttttaaaggt aataaaagtt ttataacatc
     9241 agcagggtta ggaactatgg gatttggttt aggtgcagct attggaacta aagttggtaa
     9301 tgtagataaa aatgtagttt tagtcactgg tgatggaagt tttagaatga actgtaatga
     9361 actagctaca gttgcaaatt ataatgtacc tatgcttatt ttattactca ataatagaac
     9421 attaggaatg gtaaggcaat ggcaaaagtt attttccaat caaaggtact ctcaaactga
     9481 cattaatgaa aatgtggatt atgttaaact cgtaaatgca tataatattg atggatataa
     9541 ggtatccagt atggaagaat taggaaaggc gctagatatg atagatttta ataaacctgt
     9601 atttttgcaa tgtgatatag ataaggatta tgatgtttat cctattgtag cacctaatga
     9661 tgcattggaa aatttgatat gtaactaaaa cttatactga atattgataa aattttttat
     9721 taaattttta ttggtattca gtttttgttg tttagagata gattggcatt ttttattttt
     9781 gcaaataaat caatgactat ataattaaga acttattaaa ttttaattat acaatttaga
     9841 atagtattac aacttaagta aataataatt ttgaataata atttacggag gtattcatat
     9901 gtttaaacac gataaagcat tattaaaaga agtaaaagta gaaagaccaa atcctcaata
     9961 tgctgtttta atgcaagaac agttgggtgg tgcaaatgga gagttaaaag cagctatgca
    10021 atacttgtcc caaagtttta gaataaaaga ccctcaaata aaagatttat ttcttgatat
    10081 agcagcagaa gagcttagcc atatggaaat ggtagcacaa actataaatt tattaaatgg
    10141 acatgatgtt gattataatt cagttaatac aggcgaaata gaaacacatg tattaactgg
    10201 tctatcacca gtcttaataa actcatcagg agctccttgg actgctaatt acgttacagt
    10261 tacaggggat ttagtagctg acttactttc aaatatagct tctgaacaaa gagctaaggt
    10321 agtttatgag tacttgtata gacaaataga tgataaatat gtaaaagaaa caatagattt
    10381 cttacttaat agagaagaag cacataatgc tctatttaga gatgctttaa ataaagttaa
    10441 agatacaggt tcaaatagag attttggagt tacagaagat tctaaattat atttcgattt
    10501 gtctactcca gggccaaatc acgatactaa gatagatatt aatcctcctt cttttgaaaa
    10561 acctttaaaa aaataatttg atatttttta tgttgttttg ttttagtagt tataaaaatt
    10621 atttactagg agaattaatt atatgaaaaa ttaaatatgt atggttaaaa atgggtatac
    10681 acttattata agtgtatatc tatttgttta ttgcaaaaca tataataaaa taacaaatga
    10741 ggtggtattt tggacagtaa tagaaaacct aataatctaa taaatgaaaa atctccatat
    10801 cttttacagc atgcatataa tcctataaac tggtatagtt ggaatgatga ggcttttaaa
    10861 aaagcaaaag aagaggataa gccaatattt ttaagtgtag gatattcaac atgtcattgg
    10921 tgccatgtta tggaaaaaga atcttttgaa gatgaagaag ttgctgaaat aatgaataga
    10981 aactttgtgg cgataaaagt agataaagag gaaagacctg atgtagatag cgtgtatatg
    11041 acagtttgcc aagccatgac aggtagtggt ggatggccaa tgaccataat aatgacacca
    11101 gataaaaaac ctttttttgc gggaacatat ttcccaaaat attcaaggta taatagacct
    11161 ggagttatag atttattaga gaatgtatct gaaaagtgga atacaagcag ggatatatta
    11221 ataaaatctg gagatgaaat aatagaagct cttaaggatg attttggtgt aaaaaatact
    11281 gagggtgatt tatcaaaaga gatgttgagt tcatcagtta gagtatttaa ggctatttat
    11341 gatgaaaagt atggtggttt tggtaatgct cctaaatttc caagtcctca gaatcttatg
    11401 tttttaatga aatattatag tatagaaaaa gacaaagatg tgttaaaaat ggtggaaaaa
    11461 actttagatg gaatgtatag aggaggcttg tttgatcata ttggatttgg gttttcaaga
    11521 tactctactg ataaaaagtg gttggctcct cattttgaaa aaatgctata tgataatgct
    11581 atgcttacta tagctttttt agatgcgtat aaaataacaa agaaagagtt atataaagaa
    11641 attgctataa aaactataga ttatgtagtt agagagatga aggataaaga aggtgggttt
    11701 tattcagcac aggatgctga tagtgaaggt gaagaaggta aattttatac atttaatcct
    11761 cttgagataa ttgaagtttt gggtgaagaa gatggaattt tctttaataa ttattttgat
    11821 ataactagta gtggaaattt tgaaggcaaa agtattccta atcttataaa aaataaagaa
    11881 tatgagagac ataatgaaaa gatagctgac cttagtaaaa aggtatttga atatagaaaa
    11941 gaaagaacat ctttgcataa agatgacaag atacttacat catggaatgc attgatgata
    12001 gttgctttaa caaaggcata tagtacttta aaaaatgaca tatatttaga gtattcaaat
    12061 aagtgtctta actttataaa taataatctt gtaaatgagt caggtagatt attagctaga
    12121 tatagagatg gaagttccga ctatttagca tatttagatg attatgcttt tttaatctgg
    12181 gcgtatatag agctttatga gtcaacattt aatatgaaat atttagaaaa ggctttaaat
    12241 ttgaatgaaa gctgtatcaa ccttttttgg gattatgaaa aaagtggatt ttatatatat
    12301 ggtaaagata gtgaaaattt aatagcaaga ccaaaagatt tgtatgatgg agctattcca
    12361 tcaggaaatt ctgttcaatt atacaatctt ataagacttg cgaaaattac tggagataat
    12421 agacttgaag aaatgtcata taaacaatta aaactgtatg tggataatgt aaagagttct
    12481 ccaactggct acagctttta tatgctttca ttgatgtttg aactttattc tactaaggaa
    12541 attatatgta tttttaagga agattctgat ttaattgcat ttaaagaatt gataagtgaa
    12601 aattttatac caaatgcaac ttttttagca aagaaataca atgaagaaaa cactattata
    12661 ggttttttaa ataactatat attgaaggat gataagacta gttattacgt gtgtcaaagt
    12721 aatagttgta gtcaaccaat taatgattta caaaaattaa aagatatgat tttaggagta
    12781 gaataatcat aatagcgaaa aaggagatgc tttaaaataa aagtctaatc atctcctttt
    12841 tttgtataaa aaacagaaaa agagttccta agaacaccta atctatgtaa tgattgtttt
    12901 gttaaaaaat aaaaatgaca ttatagttat ataaaatatt gtactataaa aaagtggtat
    12961 tatagatgcg atagtattaa tcttttttat ttaccctatt tcttcttttt gcaaattctt
    13021 caagtgcttc agatttaatt ttatctattt cttcatattc cataaaatta gcatcttctt
    13081 caaagccatc ttgttcttca ccattaaaac gatacatttt ttttagtaaa ctccataagg
    13141 tctctaattc atctttggta aattcgtgaa atacatctgc cataaagttt attccagtcc
    13201 tactacatgt taccataacc ttttttccta agtctgtaac tttaacatta attgctctct
    13261 tatcatgagg acttggaatt acatcaacat atccattttt ttcaagatta gcaactagtc
    13321 tatttatatt ttgtttactt gtacccattt ttcttgcaat attatttaat gttgtttctt
    13381 cctctggtaa atgaagaatt gataaaatag tcatatactg tcttgaagta agtataccaa
    13441 aatatttatc tccttctatt tgtattttgt ttgtaagggt aaaaagagtt gcatatgttt
    13501 gattcattaa aaagagctct tttagttcat ttgaataatc catgaaatat atctccttta
    13561 tttttagatg ttgttttgtc gaaaacagaa tatcatgaat taattctaat gtcaatatgg
    13621 tgtcaaaata tattaaagta ttactttttt atgtaactat taattaatta aattattgta
    13681 taattttatg taaaaacttc tatgtaccaa tgataatttc tatgaggaat taataattct
    13741 taaatttttt agaaagattt tgacttgaaa atacttgtaa tataatatat aaattaatat
    13801 actgaataaa atcaatctgg agggataatt atgaagtgta caaactttac tttcaaagga
    13861 gaggaaggtt tagatatata tacatataaa tgggaagatg aaaatataaa aaaaccaaag
    13921 gcagttatcc aaattgcaca tggaatggct gaaactgcac aaaggtatga gacttttgcc
    13981 aaggtactta ctaaaaatgg ttatatagta tatatcaatg accatagagg tcatggaaaa
    14041 actgcaaaga taatagaaaa tgttggacat ttagctgaaa aagaaggatt tagatgtctt
    14101 gtagaggaca tgtatacttt aacaaatatt ataaagaaag aaaatgaaga tttgccaatt
    14161 tatctatttg gacacagcat gggttcattt gcaagtcaaa gatatataat ggattacagt
    14221 aataatttat ctggtcttat actatgtggt tcaaatggaa agcaaggaat cattctaaat
    14281 cttgctcatc tgataatcaa tcgtgagatt aaaaagtatg gaagacgttc taaaagtaat
    14341 aaaataaata atttgatatt tggtggcgaa ataataagaa gaaatgaaaa gactaagttt
    14401 gactggttaa gtagagataa agaacaagtt gaaaaatata taaatgaccc attttgtgga
    14461 gtagtgtgta gttgtggctt tttttatgat ttagttcaag ggttaaaaga aattgaggat
    14521 aaagaaaact taaaaaaagt gccacttgat atacctattt acataatatc tggagataaa
    14581 gaccccatag gaaaaaatgg taaaggagtc ttaagactaa gggatagata tataaaatta
    14641 ggagtaaaag atgtaacttg caaactttac aaagatggaa ggcatgaatt gttgaatgaa
    14701 ataaatagag aagaagtttt tgaggatata atttgttggt taaataataa aatagaaata
    14761 ttgtaataaa aaaatataat gatatatttg ctttaaattt atttatatat aatatttgaa
    14821 aagtggatta tgtatatgca aagtagaaaa gagagagcct agaagtgatt ttttgctata
    14881 atgccattta ttgaataggt gggtgttatt ataaataaat tattaacact cagtatgatt
    14941 atcattgtat atggaattta ttttatttaa aattccttga aagtttaaat atagtaagtt
    15001 gtaataaaga gaaaatctaa atatttaaat gctatttaat gtgaatctat taattaggtt
    15061 atacttaaac ttaattaata gattttatat ttcaagccaa tgagataggg ttttagatta
    15121 aaatataacc aatggttaaa ttacagacat tttgttaact ttccctcgat ttactagtaa
    15181 atattttttt tatatactta aattacaaca gatgtactag ggaggtatat atgcaaatta
    15241 aaataggtaa agtcatacag tgtcttagaa aagaacgaaa tttaactcaa gaacaattag
    15301 caaagtttat aggtgtgtca acaccagcag tatctaagtg ggagagtgga aattcttacc
    15361 cagatataga attattgcct ttattagcag acttttttaa tgtgtctatt gataagcttt
    15421 taaattacaa gattgattta agtgaagaag aagtaatgaa aatttataaa gaattagaat
    15481 caggctttgc tagaattgaa attgatttat caacagaaga accaaaagaa gaatttagac
    15541 aagacttaga atctgtaaaa aagctttcca atatgtatat agagaagtat cctaaaagct
    15601 atttattgaa gctaaggata tgttcgctgt atcagatgta ttcatataaa tttggtaaga
    15661 gtgaacttaa tgacagagta aaagaaacaa tgaatattct tgaagatata gtgagaaata
    15721 cagatgatat ccaaataaaa gaaacagctc ttattatatt atcaaacgca tattgtatgt
    15781 tggaggatta tgaaaaggca gagttgtatt taaatatgat acataagtca ataggggata
    15841 caagtgttaa tttagctatg atatatttaa agcaaaatag acttgaagaa gcagaaatat
    15901 tgcttcaaaa taaattgttt agtaatgtat ttaatataag catggattgt aagggaataa
    15961 ttaatgtata caaaaatcaa tacaaggaat taaagaaaaa attggagaat agaaatttta
    16021 ataaaaatgc aattgagaga gaaatggagt atataaaaaa taagttgctg gggtatgcaa
    16081 atctttcttt agaaataaaa aaaatgctca gtgaagataa aggtgcattt tttagtatgt
    16141 atatggatta tatggaatta tctctaattt ttttattttt taacatgaaa gaa
//
LOCUS       DACULQ010000087        16141 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.087, whole genome shotgun sequence.
ACCESSION   DACULQ010000087 DACULQ010000000
VERSION     DACULQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.087"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            50..726
                     /locus_tag="JA154_10940"
                     /pseudo
     CDS             50..726
                     /locus_tag="JA154_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            786..2813
                     /locus_tag="JA154_10945"
     CDS             786..2813
                     /locus_tag="JA154_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT7996379.1"
                     /translation="MVDTMKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIR
                     ASNGILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSM
                     RKGTQSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYR
                     PKIKNSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSII
                     CFTTFAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVE
                     DLSYFMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVN
                     IYGKLIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSN
                     RFEPIFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTL
                     LNERKRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRV
                     RSLQEFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINK
                     EMLTRAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRF
                     FTERDNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            2863..3543
                     /locus_tag="JA154_10950"
     CDS             2863..3543
                     /locus_tag="JA154_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996380.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            4032..4505
                     /locus_tag="JA154_10955"
     CDS             4032..4505
                     /locus_tag="JA154_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996381.1"
                     /translation="MFVICISIHLAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQ
                     ILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGG
                     IGFKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVP"
     gene            4886..5803
                     /locus_tag="JA154_10960"
     CDS             4886..5803
                     /locus_tag="JA154_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAT7996382.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQGIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            5805..6629
                     /locus_tag="JA154_10965"
     CDS             5805..6629
                     /locus_tag="JA154_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HAT7996383.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            6648..7412
                     /gene="pstB"
                     /locus_tag="JA154_10970"
     CDS             6648..7412
                     /gene="pstB"
                     /locus_tag="JA154_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089777.1"
                     /note="ATP-binding protein; PstABCS is an ATP dependent
                     phosphate uptake system which is responsible for inorganic
                     phosphate uptake during phosphate starvation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAT7996384.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            7440..8093
                     /locus_tag="JA154_10975"
     CDS             7440..8093
                     /locus_tag="JA154_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate transport system regulatory protein
                     PhoU"
                     /protein_id="HAT7996385.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            8251..9120
                     /locus_tag="JA154_10980"
     CDS             8251..9120
                     /locus_tag="JA154_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996386.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            9207..10643
                     /locus_tag="JA154_10985"
     CDS             9207..10643
                     /locus_tag="JA154_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HAT7996387.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            10691..11518
                     /locus_tag="JA154_10990"
     CDS             10691..11518
                     /locus_tag="JA154_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HAT7996388.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    11814..12076
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12152..14818
                     /locus_tag="JA154_10995"
     CDS             12152..14818
                     /locus_tag="JA154_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAT7996389.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     gene            15243..15953
                     /locus_tag="JA154_11000"
     CDS             15243..15953
                     /locus_tag="JA154_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996390.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNTITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT         6383 a         1656 c         2926 g         5176 t
ORIGIN      
        1 ctataaaact tggaattata attaaaatac ctttgaatgg agtggtgtaa tgtataattt
       61 actggttgta gatgatgatg ttgatatttt aaacataaat caaatttatt tttctatata
      121 ggttttaatg tatatacatc agagactgct aaagatgcta tggatatagt agagagtata
      181 gttttagact gtataatttt ggatatttca ttgccagata tagatggata taaaatgtgc
      241 aatgatataa aagaaaaggt taacatacct ataatatttt tatctaatta tgagcatgaa
      301 gaagaaaaag ttcggggatt tttagctggg ggggatgatt atataacaaa accttatagt
      361 ttaaaagaac ttgaacttag aatatatgct cgtatgaggc agtacaaaaa tattacatca
      421 gttcctaatg tgttaaagtt ttcagcgtta gtaataaatg taaattcaag aaaagtaact
      481 tttgatggag aaagtataga tttaactact atggaatttg aaatattatt gtttctagct
      541 gagaacaaag aacaagtatt ttcaaagata gagatatata accaagtttg gaaaatgcct
      601 gatattggag atcaacatac agtgcaggtt catattgcac agatgagaaa aaaaattaat
      661 tctttaagca gggagcatca atacattcaa accgtatggg gaaaaggata taagtttgta
      721 ccataagtga gatactgtaa ttaataaata gttcttgttt tatttaatac tactatgaaa
      781 gagatgtggt tgatacaatg aaaaagtata atatactaaa aggaactctc attgtattga
      841 tagtctctct gctggcaata acaggattaa atcttctgta taaatatgat aataaatata
      901 catataagtc tattagagca agtaatggaa ttttaactat agataatgat actttcacta
      961 aaggtaaact tgtctttttg atagatgact gggagtttta taatcataaa atatttaaac
     1021 cgaatgattt tacagagcaa gatataaaac cagaatatgt ttttataggt caatatccag
     1081 atttttctat gagaaaaggg actcagtcac catatgggaa tgctacttat cgtatgagaa
     1141 taaaaaatga aggcaaggat aagctactaa gtttagagct tcctgagatt ttttccgcaa
     1201 gtgaagtatg gataaatgga gaaatggttt ctaaattagg agatgtagga acagaaaaat
     1261 atagacccaa aattaaaaat tcagtagtaa gttttattgc aaagaaaaat acagaaatta
     1321 taatcaatgt atcgaatttt tcacattatt atagtggttt gtattatccg cctgcattag
     1381 gagaaacaaa ggacataagt aatatgatgt tttatcgatt acttttttat agtataattt
     1441 gcttcacaac ttttgcaata gctatatttt cattgtctgt atggtttctt tcaaacaaaa
     1501 gtaaaattta cttatatttt ggatgtatgt gtgttttctt tgccatccat gtatcctatc
     1561 cctttgtaca tttgttagga ctgcctttgg taagctttac atatgcggtt gaagatttgt
     1621 catattttat ggtagtttta tgtattattt gtataaatgg aagtatatcc aaaatagaag
     1681 aaaataaagt gtatagattt ttaattttac cattaggaat tacaatgagt atcatatcta
     1741 tagtgatacc aattattctt cttccatatg atatgtactt tgtcaacata tatgggaagt
     1801 taatagacat atataaatat attgtattta tatatatatt aatgtcttcg ctggtttcaa
     1861 tatataaaaa gacatgctct gagcatataa gttcttatat tttagtttca gtaaacactg
     1921 tttttggaat tagcatatta tttgatgcag ttacgagtaa tagatttgag cctatattta
     1981 cagggtggca aacagaatat tgtgggtttg taattgttat tttattttct gttatgatga
     2041 taaacaggaa tagaataata ataaaagaga atgaaaaact tacaaggaat cttgaacatg
     2101 aagttgaagt gagaactaat gaacttacaa cattgttaaa tgaacgtaaa agattcttgg
     2161 cagatgtagc acatgattta aaggcacctg tttcggctat acaagcattt atagatttga
     2221 taaaggttgg taatattcat gtagatgaag agacaagaac ctatctttat gctataaatc
     2281 aaaagtcaaa tgaagtacaa aatagagtac gtagtttgca agaatttaca tcacaagata
     2341 aaagcattgg tattagtgaa aaaatatgtc tgaataattt tattaaagag atatatgaaa
     2401 ataatttgcc agatacacaa gcatgtggtg taaactttga aacttatgta ttggagaaaa
     2461 aaatatatgc aaagataaat aaagagatgc ttacaagagc atttgagaat ttaatttata
     2521 atgctttgac ctttactcca tttgatggaa agataatttt atcaatggat aaagaagatg
     2581 gatttgcaat tattaaaatt tctgataatg gaaaaggtat acctccagaa aatataccaa
     2641 aggtatttga cagattcttt acagaaagag acaataatga gccaaaaggg caagggttag
     2701 ggctatatat tgtcaaatcc attataagag agcatggtgg agaaatattt gtagattcag
     2761 aactaggaaa aggaactgtt tttacaataa aattaagtct atgtttttta taataatagc
     2821 ttatttaata taaaagaata atgatatttg ggagtgatta taatgcaaac taagatattg
     2881 ataatagatg gagacaagga taattgccag aagctaaaag gatttctgga ggaaaaagga
     2941 ataagtatag atttagcata taattgtgaa gaagctattg gaaaaatatt tagcaataaa
     3001 tatgatttaa tatttttaga aataattcta agtgatggtg atggatggac tctttgtaaa
     3061 aaaattagaa atgtaactac ttgtccaatt gtttatatga cttatataaa tgaagaccaa
     3121 agcattttaa atgctttaaa ttctggtgga gacgattact taataaagcc tctaaattta
     3181 gaaatattat acgcaaaagt aaaagctatt ttaagaagaa tgaactcata tgtaaataat
     3241 aatgaaaata atacaaaatt agaggaatat aatagaacta gtggagtaat aaagttagaa
     3301 gataaaataa taaaattgac tccaacagaa aataagttgt taaacttttt tatagagaat
     3361 cctgaaaaga ctttgactac taaagagata tatgaacatg tatggatgaa tgaatactta
     3421 gaggataact atagtgtggt agttgcagtg aatggattaa gaaagaaaat agagaaagat
     3481 tataaaaatc ctcagaaaat tataacaata agaggagcag gatattattt taataaggaa
     3541 taataaatat attaatatca ttaataattg ttataaatgt gaaatactaa cgatatctaa
     3601 actaaattat tataaaatag ctgactgtaa tttaattgat aaatcctatt cttctagact
     3661 ttgccagtta aattacagtc agttttatta tataaaaaaa tgcgtaatgg aactattatt
     3721 ttatagatta tatttgaaaa gatggataaa ttgtatatct aaatctatat attattttat
     3781 tatttaatat atattaaata ataaaatagt taaataataa gattaagaat ttcttaataa
     3841 atccagaaga tgctttcgat tatataatat tttatatttt tatattatta attatatgtg
     3901 ttgcaatcgc tgcacctact ttttctggtg agtatcagac acaatcagat agcatccttc
     3961 gttgtacgaa acatggacat attcgtctag cgtttactaa aatattagct ctattttcta
     4021 tatttataat aatgtttgtt atatgtattt ctatccattt ggcaatttct gatttagctt
     4081 ttggtactga atgtttgaaa acctcatttc aaatgttgtt ttctgtcatt agtctagtaa
     4141 atataaattt actacaagca cagattctta tagctattgg tggtctaatt tcaatacttg
     4201 caatggttag tttaacatta tttttatctg caaaatgtaa ggattctctt acttctatgc
     4261 tgattgcttt ttctatttgc ttaattccta tgggaatagg tgttagttgg attggatacc
     4321 tattaccacc agggggaatt ggttttaaga ttagtttatt atatcaaatg acatcattta
     4381 atttcttaca cttaggaggt atgagttttt ggacaccaca cattatactg tttttctcag
     4441 tgatagaaat acctattttt atatttttgg ctattcgtac ctattgcaaa catcaagtac
     4501 cgtaacaatt tgatggaaat aatagtgata taaactagag gatacaataa ttatatatgt
     4561 agtataaaag ttaaatttag ttaacaaatc ttaatagcag atataggtaa taaattatac
     4621 ataaactgta ttattatcta tatctgctat tttaatatag agaaacaaca gtatattgta
     4681 aagtaaatgt caaatatagt tttaaaagta aaattaatgt taaaaagtaa atataatttt
     4741 acacttgctt aacttaatcc taatatagat ttaacttaaa aaaagtagaa taaaagtata
     4801 ggaaatttga tgaagacgaa ttataaaaaa ttaaattttg aatacagaac gaaaatcgat
     4861 aagtatattt taatgggagg gtattatgat agtaaaatct agattagtag gtttagaaaa
     4921 aaatgacaat aatggcaata agacaaaata cgtaattgaa aaagtagcaa aaaatatttt
     4981 tctacttagt gctttagtag cagttgcaag tttacttttg ataataggat tcgtatttta
     5041 taaaggatta agaccattta tatttgaagg atattcattt atagactttt taactggtgc
     5101 agattgggtt ccttctgcca ataaatttgg aatatcatct atgatagtag catcaatatt
     5161 tgcaacactt ggagcacttg ttataggagt acctgttggt atattaacct ctgtatttat
     5221 agcagaagta gcacctaaaa gattagcaaa aataatgtca ccagcagttg aacttttagc
     5281 aggtatacct tcagtattat atggtgtatt tggtcttgca gtgatagttc caactataca
     5341 aggtatattt aatcttccaa aaggtcaaag tttgttagca atagtaatag tattagcagt
     5401 aatgatgtta ccaacaataa tatctgtatc agaaacagca ataagagcag taccaaaagc
     5461 atacaaagaa ggttcattag cacttggtgc atcaaaaata gaaacaatat tcaaagttgt
     5521 attacctgct gcaaaatctg gagtattagc cgcagtagtg ttaggagtag gaagagcttt
     5581 aggtgaaaca atggcagtaa tacttgttgc aggcaactca ccagttatgc caactgcaat
     5641 aacagatagt gtaagaccac ttacaactaa tatagcactt gaaatgggat acgcatttgg
     5701 tacacatcaa gaaatgctat ttgcaactgg agtagtattg tttacattta tattaatttt
     5761 aaacttagtt ttaaataaac tttcaaataa ggcggtaaat taagatgaga aagttcaaag
     5821 agaatctatt gaagtcatta gtatatctat cagcactatt tacaatagta tcattagtag
     5881 ttatagtggg atttatattt ataaagggaa ttggcaacat gaattttaat ttcttattta
     5941 gcaattattc agcatcaggt gatggtggta tacttccaat gataattaca acattatata
     6001 cagtagtagt ttctatagtt gtagcaactc ctataggaat attggctgct atatatttac
     6061 aagaatatgc taaaaaagga aaagcagtta cagctataag atttgcaact gaaagtttat
     6121 cagggatacc atctatagta tatggattat ttggaggaat tttctttgta gttacattaa
     6181 aaatgggata ttccatagtt gcaggttcac ttacagttgc aataataata ttaccagtaa
     6241 taattcgtac aacagaagaa gctttaaaaa cagttcctca agcttataga gaatcatcat
     6301 tggcattagg agctacaaaa tttcaaactt tatataaggt agtacttcca agtgcaatac
     6361 caggaatttt atctggagta atcttatcag ttggtcgtat aattggagaa tcagctgcaa
     6421 tattacttac agcaggtact gtagccaaga tgccaggtgg aatatttgat agtgcaagaa
     6481 cattaactgt acactcatac ttactgacta aagagagtgg agatatagca acagcagcca
     6541 gtataggaat tgttttaata gtaattgtat tagcactaaa catgctagca agatttgttg
     6601 ctaagaaatt aaataaagct aattattaaa tttaagaggt gtgaataatg gaattaatcg
     6661 ataagataaa aatgagtgta aaagatttgg atttatttta tggtgataag caagcattaa
     6721 agaaaataaa tatggatata aaagagaata aggttactgc tttaataggt ccatctggat
     6781 gcggaaaatc aacttttata agaacgttaa atagaatgaa tgacttaatc gaagatgtaa
     6841 caattaaagg aaacatttct gttgatggag aggacatata tacctcagat gatgttataa
     6901 atcttcgtac taaagtagga atggtatttc aaaagccaaa tccatttcct atgagtatat
     6961 atgataatgt ggcatatggt cctcgtacac atggactaag agataagaag caattagata
     7021 aaattgtaga agaaagctta aaaggtgcag ctatttggga tgaggttaaa gacaggctta
     7081 aatcatctgc actaggactt tctggtggtc aacaacagcg tatatgtata gcaagagcaa
     7141 ttgctatgag accagaagtt attcttatgg atgaacctac atctgcttta gacccaattt
     7201 caacattaaa agtagaggaa cttatagagg atttaaaaaa agactacaca atagtcatag
     7261 ttacacataa tatgcaacaa gctgctcgta tatcagatga aacagctttt ttcttaaatg
     7321 gagaggttat agaatttagc gatacaaaaa ctatgtttac cactcctgta gataaaagaa
     7381 cagaagacta tataacaggt agatttggtt aaaatatata actataaagg agttagacaa
     7441 tggtcaataa tagtttagat ataagtattg atactttaaa agaacaaaca gtgcttatga
     7501 tggaaaaatg tgaagaagca atagaaaagt ctgtgacttg tatgatagag aaggatttag
     7561 agggtgcaag aaaagtagta gaagatgatg aagaaataaa taaattaaga gaagaaataa
     7621 gagataagag tatagagcta atagtattaa agcaaccaat ggcaagggac ttaagattta
     7681 tttattcatt aggtaatata tctatagagc tggaaagaat aggagattat gcagcaaata
     7741 tagctagaga aatgttgaag ataggtggag aggcttatat aaaagagcta atagatattc
     7801 ctaagatggc aaaagaatgt aagtctatga tgtctcagac tagagaagct ttaatcttat
     7861 ttgatgcaag tatagcttat gatgttggaa ctaaagatga tattgtagat ggattgtatg
     7921 aacaagtaca aattgattgt ttaaaaataa tgcatgaaaa ctcaaatacc atagaccaag
     7981 gggtaaaact tctacttata ggaagatacc ttgaaagaat tggagaccat ataacaaatg
     8041 tatgtgagaa gataattttt gcaattgagg gtaaaatggt tgagatagga taaaaaactg
     8101 ctttaaagca gtttttttat tgtctaaatt aagaaagaat tgataaaaaa atatgattaa
     8161 tgggtaaatt taaaaaattg tgatatacta ggaacatatt aaatatgagg atgcgtattt
     8221 tatataaggt gctttttagg aggtggaact ttgaaacttc aaaagatggg tatgaggact
     8281 ataaaaacag gaattagtgt cactttatgt atgattcttg gtgactattt agtagagaat
     8341 atgttttatt ctctggtagc atgtataatt tctgttcaag atacagtaaa aggttcttta
     8401 aagacgggaa tcaatagagt ggaaggtact attttaggtg gtataatagg attcatattt
     8461 gcaatcatta aagctggaga cccattttta tgtggtctag gagtaatgtg ctcaatatat
     8521 ttatgcaatc tgtttaaaat aaagtcagta gtagttgcct gtgtaacctt tctggctata
     8581 catctaggtg taggaaatag tgaccctgtg tattattctt tacatagggt gttagataca
     8641 tctgtgggtg ttttgtttgg agtacttgtt aattattatg ttgctagacc aaattatcta
     8701 aatagtgtta tagatgagtt taaaaagata gaagaacttt caatgttgct tattgaagat
     8761 aaaattctta gaaaagaaaa tttaaatgta gagaaatttg aagaagaagt agaaaaatta
     8821 gaagaaatat attcaaaact tattgatgag ttggattata gtttgagtga aactaatata
     8881 gaaaaaattg agaatgcctt aaggatatgt agagaaatac attttcatat gcagtctata
     8941 gagttacttg aaaataaact ttatttgaaa gagcatacat ataaatacct taagaaaaca
     9001 tataatgtgg ataagctaga ctggaatata gatgaagaaa aaagccctgt attcaactat
     9061 catctaatga aaataatgat tgaaatagag ttgttgagaa agctaaataa atcatattaa
     9121 taatagtagt taaataataa ataaaaaata gtatctttgt aaatattaaa catatataag
     9181 tttaatttat ggaggtacta ttttttatga atactagaaa tgacaattct aaaatactag
     9241 aaatatataa aatgaagctt tttggcgaac ttgtaaaagc agtgtggaat gatgatatag
     9301 aggatattga aaacttacct aataaaattt taaatgaaga tttaaaggat aatttaaata
     9361 attattttag tggaaaattt gataaagata ctatggtaaa tttaattagg gtaactatgg
     9421 gattggatgt tacagaagat acagataaaa atttaaaaga gtcactgcat gaagctgtaa
     9481 atttagatga agtcaaaaga ccaataatat ctgtaatatc agatgcttgc aaatattgtg
     9541 atgaaaataa taaggaagat gagtcatgta agataagaag taagcatatt aattgtaatg
     9601 aggaagatgt atgtagtgct tgtggtgatt gtgtatcaag atgtaagttg ggtgcaattt
     9661 cagataaaat agaatttata ccaatgataa aattgttaaa agatataaag agccctgtat
     9721 atgcaatagt tgcaccagct tttgtaggac agtttgggaa agatgtaagc cctggtaaat
     9781 taagaactgc attaaaaaag ataggatttg aagatatgat agaagttgct ttggcagcag
     9841 atatgcttac tgcaaaagaa gcatatgatt attatgagca tatgaaaaag gacgaagaag
     9901 gatgctttat aactagttgt tgctgtccta tttgggttag tttgatacaa aatagttttc
     9961 ctcagatatt ggaaaatgta tcaccatccg tttctcctat gattgcttgt gggagagctg
    10021 taaaaatttt aaatccagat gctaaagttg tattcatagg tccttgtatg gcaaaaaaga
    10081 aagaagcaat gttagaagat ataaaagatg cagttgattt tgtgcttaca tttaaagagt
    10141 tgcaagaaat atttgatgca ttagagttaa atccagcaat aatggatgag agtaatagag
    10201 tagaggcgtc cctttcaggt agagtttatg ctagaacagg aggtgtaagt aaagcagtta
    10261 agttatcagt tagaagaatt gacaaaaata taaaatttga agaaaaggca tttgaaggaa
    10321 caaaagagtg catggacggt ctaaagaaag ttctaaataa agaaatagaa gctacattta
    10381 tagaaggaat ggggtgtgta ggaggctgtg taggaggtcc gaagcgtatt ttaagtgtgg
    10441 aagaaggtac taagaatgtt gatgaatata gtgaaagtac gaatatggaa actccatttg
    10501 ataatttaaa tgtattacaa tttttaactt ctttgggaat caaaagagta gaatctttag
    10561 gagaaaaaga agaagaaaag gttaaaaaaa tatttagtag agatataaaa agtaataaga
    10621 aagagaataa atataaaaaa taacttttta agtcttaaac ttatgctttt ataaaaggga
    10681 gatatggggg atgaaaatta aagataataa ggctgttttt catgtattta taaggatatt
    10741 tcttatagct gttttaattt taactggaac attattggag gaaaaaatta ataatgttcc
    10801 agttgaaaat ataagttcaa aaggtgaaaa tacactggaa ctgctttctc aaagtaatac
    10861 tttaggagaa atcatataca aagcatctca agaagaaaca aaaggtccag gggctacttc
    10921 tgaaaaagta gcatacataa caatagatga tggtccatca aaatatacaa atgcactact
    10981 tgatatttta aaagaaaatg atgtcaaagc gacatttttc atgcttaatc agaatatgaa
    11041 aaaccacaag gaagaagtaa ggagagtctt ggaagaagaa aactcaatag gttttcatag
    11101 tgtaagtcat gatatacata agttatataa aagcccagaa gtaacagttg gggaatttaa
    11161 tacatgcaat aatactctac aagaaataac tggtcagagt tcaaaattaa taagattacc
    11221 atacggaagt aaaccatata tgccagaagg ttcatataat aaacttatgg caaatgatta
    11281 tttaatttgg gactggaatt tggatacaca ggattggaag tcttctacaa gtcagatagt
    11341 aagtaatata ttatattatg gaagaaaaag agatgaatta gtagtattaa ttcatgagaa
    11401 agagcaaagt ttaaatgctt taaataatat tataagaatc ttaaaagaga gaggatatac
    11461 aatattgcca attacagaaa atataacacc taaaaatttc tggcaagaaa acttataata
    11521 aatttaaata tagtaaaaga taaagtaaat tatttataaa ataaaacata taatataaaa
    11581 aatacgaata agaggtatac tgtaaggata atataaaatt aatgagaaag cattaaaatt
    11641 aaaataaaat aatcttgcaa ttattgacat tataagacag aataactgta aaaacatatt
    11701 ttaaaatata acaactatat aaaagagaat agacataatt aagaaaattc agaaaaaata
    11761 aaataaaaat aattgacaaa gagaataata tttgtatata ataatatgta atattaaaga
    11821 ctgagaagaa gaggagtaag taaatactac taaaagagag gaaaattcac tggctgtaag
    11881 attttctaag tataagattt actgaatgta gctttggagt ttctaaactg aactataagt
    11941 aagtttagac ggttagaacc gttaaattct ttgagagtca aagttgataa aataacatta
    12001 accaataatt taattatatg ttaatgtatt ttaagctttg gaattaaggt ggtaacgcga
    12061 gcttttcgtc ctttttaaag aggatgaaga gctctttttt atttctaaag atatttaaaa
    12121 aatttattta taaaatacag gaggcaggac aatggaaaat acgaatttat caaaaactta
    12181 taacccaaaa gattttgagg ctaggctata taaaaaatgg atggatgaag ggtattttaa
    12241 atctaaacca aatccagata aaaaaccatt tacaataatg atgccaccac caaatataac
    12301 agggcaattg catatgggac acgctcttga ccatacactt caagatatat taatcagatg
    12361 gaaacgtatg gatggatatg aagctttttg gcttccagga actgaccatg cttctatagc
    12421 aacagaagtt aaagttgttg aaagaataaa aaaacaagaa ggcaagacaa agtatgaaat
    12481 aggtagagaa gaatttttaa agagagcctg ggaatggaaa gatgaatttg gaggaaaaat
    12541 atctaatcag cttaaacaac taggtgattc ttgtgattgg gataaagaaa gatttactat
    12601 ggatgaaggg tgtaatgaag cagttataga attttttgta agcctatatg aaaaaggtca
    12661 catatacaga ggaaatagaa taataaactg gtgcccagat tgcaaaacta ctttatcaga
    12721 tgcagaagtt gaacatgaag aacacgacgg aaacttttat catataaagt acccactaaa
    12781 agatagtgaa gactttttag aaatagctac aactagacct gagacaatga taggagatac
    12841 tggtatagca gtaaatccag aagatgatag atataaacat ttaataggaa aaacagcaat
    12901 acttccatta gtaggaagag aacttccaat agttgcagat agttatgttg atttagagtt
    12961 tggaactggt gcagttaaga tgactccagc acatgaccct aatgattttg aagtaggatt
    13021 aagacataat cttgagcagt taaatactat gaatgaagat ggaactatga atgaagtttg
    13081 tggaaaatat gaaggtatgg atagatttga gtgtagaaaa gctatcgtag cagacttaaa
    13141 agaacaaggt tacctaatta agataaaaga gcataatcat aatgtaggaa cttgttatag
    13201 atgccataca gtagttgagc ctagattatc agagcaatgg tttgttaaga tggaagaact
    13261 tgcaaaacca gctatagata ttcttaaaaa aggagaatta gaatttgttc cagataaatt
    13321 tgataaaact tatttacaat ggctagaaaa tataagagat tggtgtatat caagacaatt
    13381 atggtggggt catcaaatac cagcttatta ttgtcaagag tgtggagaaa tagtagttgc
    13441 tagaaaaatg ccagataagt gtcctaaatg tggaagcact cattttaaac aagatgaaga
    13501 tgccttagat acttggttca gttctgcatt atggcctttc tctactctag gatggccaaa
    13561 taagactgaa gctttagatt attattatcc aacaagtgta cttgttacag gatacgatat
    13621 aatattcttc tgggtagtta gaatggcatt tgcaggtatg ttctgtatga atgaaaagcc
    13681 atttgaccat gttttggttc atggattagt aagagattct caaggtagaa aaatgagtaa
    13741 atctttagga aatggtatcg acccattgga gataatcgag caatatggag ctgatgccct
    13801 aagatttaca ttaacaactg gaaattcccc aggaaatgat atgagattct atatggaaag
    13861 agtagaattt gctagaaact ttgcaaataa gttgtggaat gcatcaagat ttgtatttat
    13921 gaatattgat gaagatataa ttaaaaacat gactagagaa agtgttaaag aggacttaac
    13981 tctagctgat aaatggataa tatcaagagc aaataatatt gtaaaagaag caactaataa
    14041 catggataag tttgatttag gaatagcact tcaaaagata tacgatttta cttggtcaga
    14101 atactgtgac tggtatattg aaatggttaa acctagatta tatggggaag atgcaaatgc
    14161 taaatctgca gcattatata cattaactta tgttcttgaa aagatattaa aattattaca
    14221 tccatatatg ccatttataa cagaggaaat atatactcat cttcctactg ttgaaggatg
    14281 tataatagta tctgaatggc caaaatataa tgaagaagat aacatggctg aagaagaaga
    14341 tatgatgaac ttattgatgg aaggtataag aagtataaga aatgtgagag cagaaatgaa
    14401 tgtaccacct tctaagaaag ctaaattgat aataatccca agtgaagaaa aaatagaagc
    14461 tattgagctt ggtaaagact attttataac tttagcatct gcttcaaatg tagagatagc
    14521 aaaagataaa tctaatgttc cagaagatgc agttggagta gttattgatg gtgttgaaat
    14581 atttatacct cttaatgaat tagttgattt tgaaaaagaa attgagagat tatctaaaga
    14641 aaagaaaaag ttagaaggcg aaataaagag agttaatgga aagcttgcaa atcaaggttt
    14701 cttagcaaaa gctcctgaaa gtttaattga agaagaaaaa gcaaaaaaag aaaaatttga
    14761 agagatgatt aaatctgttg aggaaagatt aacaaattta gaatctaaaa taaaataaat
    14821 ataagaatta gtaaaaacat acaaaaagta tatattctat actttttgta tgtttttctt
    14881 tatatatata caaatatgat gttataattc tttacagatg tgttatattt ttattttatg
    14941 ttgtgaatat atgaatttta tgtcttaaaa tttataagca ataaaaattt gatatattat
    15001 gatgtttaat atagggtaat aaatacatat tatatttatt ttactatatt tttttacata
    15061 atatatctag aaaagtatag gaatttatcg taatttaagt ttaaatatta aagagaattt
    15121 ctataagtag tttaatcgac aatttaaatt tataaagaaa tttaaatttg aaagctagtg
    15181 agtacagttt ggaaattgaa ataaagtaaa ctaaatgata ttttagattt ggaggtgata
    15241 ttatgttcag ggtagttata tgtgatgacg aaaaaataca aagaagcata ctaaaagagt
    15301 ttacagaaaa acttttgtct gaaagatgtc taaattatca aatactggaa ttttcctgtg
    15361 gagaagattt aatatccaaa tacccagaaa aaattgatat tatattttta gatatccaaa
    15421 tgaaagatat caatggaatt gaggtggcta aaaaaataag aaagtttgat gaaaaagtag
    15481 aaattatatt tacaacggct ttttcagagt atgcccccca aggttatgaa gttcgtgctt
    15541 atagatattt agtaaagcct atagagtata gtaactttgc aatgggtgta aatctttgta
    15601 ttgataacct tcaaagaaag aaggagcgtt atatagtttt aaatagtaaa aaaggattta
    15661 atagaatact gatagattct attttatatg ttgaaactgt aaagagagat ttagttgtac
    15721 atacaatagg tagagattat aaagctaata tgagcatgaa acaagcagaa aaattactgt
    15781 gtgaaaatgg cttttttaga tgtcatacaa gttttattgt taatttaaga agaatagaag
    15841 atcttaggga taatacgatt actattaatg aacaatttat acctataagt aagtatagac
    15901 ttaaagattt aaaagtagca ttaacaagct tgttatgtga tataatgtat taattttaaa
    15961 aaggtaattg aaaatatcgt aaaaaagatt taatgtcatc acagggctat tgattgaaac
    16021 tgtcttaata tataaaatgt tatttatatt aagacagttt tattatgcaa aatagcagtt
    16081 tatagacttt taatatggtt taatatgtat atgtagagtt tccattataa tagcatgatt
    16141 a
//
LOCUS       DACULQ010000088        16135 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.088, whole genome shotgun sequence.
ACCESSION   DACULQ010000088 DACULQ010000000
VERSION     DACULQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.088"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(2..205)
                     /locus_tag="JA154_11005"
     CDS             complement(2..205)
                     /locus_tag="JA154_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small acid-soluble spore protein"
                     /protein_id="HAT7996391.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            496..1260
                     /locus_tag="JA154_11010"
     CDS             496..1260
                     /locus_tag="JA154_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HAT7996392.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            complement(1378..1515)
                     /locus_tag="JA154_11015"
     CDS             complement(1378..1515)
                     /locus_tag="JA154_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996393.1"
                     /translation="MPTFKQTYTLWKYCLTAFINSSKFLNPSKKSQLLDTFLINLIYN
                     Y"
     gene            1649..2962
                     /locus_tag="JA154_11020"
     CDS             1649..2962
                     /locus_tag="JA154_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HAT7996394.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      3366..3451
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            4033..6282
                     /locus_tag="JA154_11025"
     CDS             4033..6282
                     /locus_tag="JA154_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HAT7996395.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTSDVEKPSDGQVPDGEKPSDSQVPDVEKPSDGQVPDGEKPSDSQVPDGEKPS
                     DGQVPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQ
                     TPDVEKPSDGQMPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINK
                     IVYNFWEDEEDVKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYH
                     DLRFDDITKTLTFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSP
                     YLPAEDFNGQENQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWI
                     ILGQMDEQERLKQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAG
                     REELKPNNTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLKLPDGVTAYDG
                     DGNIIGTGEVEISTQQKFKLKSVGKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKD
                     HSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPT
                     DDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPP
                     VPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSYVFISVIAICGL
                     AYQCKIKRN"
     gene            6689..8449
                     /locus_tag="JA154_11030"
     CDS             6689..8449
                     /locus_tag="JA154_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAT7996396.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            8730..8861
                     /locus_tag="JA154_11035"
     CDS             8730..8861
                     /locus_tag="JA154_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAT7996397.1"
                     /translation="MTLSEKKIILIITNYVGFNLLNFSVKGLPVEELRFKIINKKIM"
     gene            8878..10758
                     /locus_tag="JA154_11040"
     CDS             8878..10758
                     /locus_tag="JA154_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HAT7996398.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            10839..11123
                     /locus_tag="JA154_11045"
     CDS             10839..11123
                     /locus_tag="JA154_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996399.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            11161..11886
                     /locus_tag="JA154_11050"
     CDS             11161..11886
                     /locus_tag="JA154_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:11611..11613,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HAT7996400.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            11966..12724
                     /locus_tag="JA154_11055"
     CDS             11966..12724
                     /locus_tag="JA154_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HAT7996401.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            12739..13206
                     /locus_tag="JA154_11060"
     CDS             12739..13206
                     /locus_tag="JA154_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HAT7996402.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            13224..13706
                     /locus_tag="JA154_11065"
                     /pseudo
     CDS             13224..13706
                     /locus_tag="JA154_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089755.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
     gene            13726..14733
                     /locus_tag="JA154_11070"
     CDS             13726..14733
                     /locus_tag="JA154_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HAT7996403.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            14853..15734
                     /locus_tag="JA154_11075"
     CDS             14853..15734
                     /locus_tag="JA154_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAT7996404.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         6414 a         1932 c         3096 g         4693 t
ORIGIN      
        1 tttattttcc agccatttgt ctttcagcca tttcaactaa tttcttagtc atatatccac
       61 caacatatcc attttctcta gctgttaagt tacctttgtc tatgttttca tagttagata
      121 aacctatttc gttagctatt tctaatttca tttggtttaa agcagctttt gcttctggaa
      181 ctactgttct gtttgaattt gacatagtaa atttcctcct caaataatta gtttatgatg
      241 tctaacaaca cttttattta tgtgatgtta atactaattt ctccaaaata aaaataatat
      301 atacagaaaa ataaaggtaa aaatgtaaaa aatataaaaa aattaaataa atgagtattt
      361 tagagagttt aattaggtca atataggttt tattttttta gttattgtct tataaattaa
      421 atattacagt acttttttga gctgaaaaaa aataaatttt aaatatagaa gaaattattt
      481 caaaggggga atagtatgaa aagagaagtt aaaaggacta aagctttgat atatagtgtt
      541 ttattttttt cattattatt agtgttaata tttgtttttg tttatggaat aaaatacata
      601 tgttctaaat ctgacaattc aaaaatgcct atatataaag ttgatacaaa tgagaaaaaa
      661 atagctttga gttttgatgt agcatgggga actgataata tggatgatat tttaaaaatt
      721 ttagataaac ataatgtaaa agcgaccttc tttttagttg gtagttgggt ggatgataat
      781 gaagaaattg taaaagctat agatgaaaaa ggacatgaaa ttggaaatca ttcaaatact
      841 catgccaatt taaaggaaat atcaaaagaa gacataacaa aagagataga aactacatca
      901 gaaaaaatat ataatataac aggaaaaagg actaatttat ttagacctcc ttttggggac
      961 gtaaacaata aagctatgga catttgtagt gatttaggtt ataaagtaat taagtgggat
     1021 gtagattcta tagactggaa ggagcttgga ccaaatcatg taattgaaaa agtaataaaa
     1081 gaatctcaac ctggttcaat agttttattc catgctaata taaatgatgt agataattat
     1141 ttggatacta taataagtcg actaaaaaaa gatggataca gtctagtcaa aatatctgat
     1201 ttgttgtata aagataatta tgtagttgat tccaatggag tgcaaaaaac taagaattaa
     1261 taagtaataa atgtatattt taagtaaagt tgtgataaat tatatagtat atgttagaaa
     1321 agtgtcgata aaaagacggt gtctattttt tgacactttt tatacatgta tgcaatatca
     1381 ataattatag attaaattga ttaaaaaagt gtctaataat tgactttttt ttgatgggtt
     1441 taaaaattta gaagaattaa tgaatgctgt aagacagtat ttccaaaggg tataagtttg
     1501 tttaaaagtt ggcatagaaa ttgctttgtt aataagcgat atgttctata gaaataaaca
     1561 atatgttaag accttataat agaaaaatag cattaacatt atttgaacag cttataaaaa
     1621 aattaatata cttaaggggg cgtactagtt gaacaaacta tattctattt tattaaaaca
     1681 acacgttggt ggtccagata agccagtagt aagtgttgga gatgtagtaa aaaaaggaac
     1741 tctaatcgca gaaccaactg gattaggggc aaatatttat gccagtgtta gtggaaaaat
     1801 aagtgaaata aatgaccaag cgatagtaat tgaagctgat gaagtacaag aagatacttt
     1861 tgaaccatta aaaggagaag gtatacttga cttaattaaa gaagctggag tagtaggaat
     1921 gggtggagca ggattcccta ctcatgtaaa attaaacata gatttaaatg gtggaacaat
     1981 attagcaaat gcagctgaat gtgaaccttt attagctcat aatataaaag aaatagaaga
     2041 aagaccagaa atagtttatc aaggtataaa atatgctatg gaagttacta atgctggaaa
     2101 aggtatgcta gcaataaaaa gtaagcatcc aaaagcaata gaagctttca aaaaagtaat
     2161 aaagccagga gacaacatag aagttgcaga actagttgat atgtacccaa tgggagaaga
     2221 gagagcaata gtaagagatg ttctaggaaa attacttgag ccaactcaat taccttcaga
     2281 ggctaatgca gtagtaataa acgtagaaac actaactaga atagtagagg ctgttgagca
     2341 aaaaagacca gttatatcta aaaatataac tgtagttggt caattaaaca gtggaaaaga
     2401 atctatagtg tttgaagatg tgccaattgg aacaactgtc ggagaattaa ttgaaagagc
     2461 tggtggaata aaaggtgaat atggtgaaat aatcctagga ggacctttca ctggtaaagc
     2521 tactacttta gatgctccaa taactaaaac tagtggtggt ataatagtta ctatgccatt
     2581 tgttaatgaa aagagaaaaa tgggtctttt agtttgtgca tgtggaccta atgaagaaag
     2641 aatgagagat atagcaacta aaatgggagt tactgatata gtatcagttc aaaaatgtaa
     2701 acaagctcaa gaaattaaag gtgcattaaa atgtgaaaat ccaggacact gtccaggaca
     2761 agctcaaaaa tgtattgaat ttaagaaagc tggagcagaa gttatattaa taggaaactg
     2821 tactgactgt agtaacacag taatgggttc tgcaccaaaa ttaaaattag gtgtatatca
     2881 tattactgac catgtaatga gaacagttaa tcacccatta ataagaagaa taaaaggtga
     2941 cgtaagatta aaaataaaat aatagataaa tagaagttag acatctttag aagtaaaact
     3001 taaaagttac ttctaaggat gttttttaat gtattttgag ggcaatatat atattgtaaa
     3061 ataaatggaa aaaatagaca aaatatgcta gatattgtat aatgataaca atagctatta
     3121 caattaatat tggacaaaaa taatgatact caagtatgtg gtttgtatta atatgcatat
     3181 tatattaagt caactatccg atagagtaaa aggtttttgt gatatttgaa gatactctat
     3241 agtatctttt ttattataaa aactaatttt tgtcttttat tagataagta tctaggatac
     3301 taatttatat tttattgagt tgttaaaaaa gtgcacttaa aaagttaaaa aagaaaaatt
     3361 aaaaacctat ttatagaaac tgtgaagtat atcttaaacc tgggcactta aaagatatat
     3421 ggagttagta gtgcaacctg ctataaatat aaataggagt aacttttaat tgtcaaaaat
     3481 atataaaata aatacataaa taagtaatta atagaagata gatataaata tatttttgaa
     3541 atgctccctg cattcataac gagtgtagag tggcagatat ctgcccaaaa ctcctttaat
     3601 tgctggaagt tcctaaagct aactaaacca caacataatc atgaaataag gataagtgtg
     3661 atagagtgtg catttaataa gcacttgagg ttacgaaagt agaaaaaatt agttagatag
     3721 cataaggtta aatcctaagt gccttgaaaa tggataatca gcaggtaagc ttcgaaaaga
     3781 agaaacttca acgactagag agaaatctcg tacctacaag tgtggggaaa tggggagtac
     3841 ctaatttatg gttaggtaat gatatagtct gtgcttaata gaaatattaa gaagttcata
     3901 ggagaactgg ttaggagtaa cgaccctaat tgaacacact aaagatgtat tattgaataa
     3961 gattcatata atatttaata attttaatta tattaagtga atacaatata tgtttttagt
     4021 atcaggagaa ttttgaaaac aaaaattaaa aaatcaagta taatcagttt agtaattgct
     4081 tttagtatgg tatttactgc atttacacca atagtatctt atgctgacga agtgactagc
     4141 aatgatacaa tacttaatgg agaagaacaa tcaaatggtc agacatcaga tgtagagaaa
     4201 ccatcagacg gtcaggtacc agatggggag aaaccatcag acagtcaagt accagatgta
     4261 gagaaaccat cagacggtca ggtaccagat ggggagaaac catcagacag tcaagtacca
     4321 gatggggaga aaccatcaga cggtcaggta ccagatgggg agaaaccatc agatggtcag
     4381 ataccagatg gggagaaacc atcagatggt cagataccag atggagagaa accatcagat
     4441 ggtcagatac cagatgggga gaaaccatca gatggtcaga tgccagatgg agagaagaca
     4501 tcagatagtc agacgccaga tgtagagaaa ccatcagatg gtcagatgcc agatggggag
     4561 aaaccattag atgagcaaac tccagaagaa gaaaaaccat tggaagaaga aatagttata
     4621 gaggaaatga gtttaaaaca agatattgat aaaattcttg atatgacttt atctcaaata
     4681 aataaaatag tatataactt ttgggaagat gaagaagatg taaaggcaga tgaacaatca
     4741 gaaattaacc aagtttttac ttcagaagac tcatttatta gcttatggta tgacaaaaaa
     4801 gcaaaagtaa aaaattcttg tttactaaaa gaaatagatg gttcagatag accttatcat
     4861 gatttaagat ttgatgatat tacaaagact ttaacttttg actatgtaat aaagggtctt
     4921 gtaggagcaa gctcaaaaga cgacaaatat gtaatagaag gtgaagatgg agagcaaact
     4981 gcattttgtt acaataatca tttaagacca ccaagtagta atggaaagag tccatattta
     5041 ccagcagaag attttaatgg tcaagaaaac caaaatgaag aagcagttaa atctatacta
     5101 tatgcaggtt ctgaatttga tggatttgga tataagcaac aatttaattt aggtggagaa
     5161 gaaaatgaat tgatgactta ctcagctact caaagtgcaa tttggattat tcttggacaa
     5221 atggatgaac aagaaagact taagcagtat caaggttcta taaacctttg tgataaactt
     5281 atagaaagag caaaaactga agaggaaaga gctgagtatc agaagaagaa agaagtagcg
     5341 ataaatataa aagcttattt agaagcattg ttaaaagcag gtcgtgaaga gttaaagcca
     5401 aataatacag gaaaacctag tttgagtaat ggtttaacta agattaactt tgaaaaaaat
     5461 gaagatggta cttatgaaac agaagctgta gccttagtag gttatagtgg tgtagtaaag
     5521 ttaaaactac cagatggagt aactgcttat gatggagatg gtaatataat aggtacaggc
     5581 gaagtagaaa tttcaacgca acagaaattt aaacttaaga gtgttggaaa accagatgcc
     5641 aaagctaata tttctgctgt atcttatgat tatatttttc caaaagctat tcaatattat
     5701 aaggcagtgc ttgatttagg acaaaaagac cattcatctt cattaccatc tagcaagcaa
     5761 aatttattaa gttatacaat agagaaaaag aatggtcaag aagttaactt taatataggt
     5821 ttaccaacag atgatgatga tgttgtaaat ccaccagtgc caccaataga tgatgatgtt
     5881 gtaaatccac cggtgccacc aacagatgat acaaatggac ataagccaaa accatcacca
     5941 ccaatagatg atacagtaat aaatccacca gtaccaccaa tggatgatac aataataaat
     6001 ccaccagtac caccaacgga ggatgcagtg ttaaatccac cagtgccacc aatggaggat
     6061 gcagtgctaa atccaccagt gccaccaaca gaggatacag tgttaaatcc accagtacca
     6121 tcagtaagtg atacagtgga aaaaactcca gaactatcaa gagatgatac aataataaaa
     6181 tcaccaaaaa caggtgatga aactcagcta atgtcatatg tatttattag tgtcattgca
     6241 atttgtggat tagcgtatca atgtaaaata aaaagaaatt aatctagatt attattaagt
     6301 ttaaaataaa aagtctcaaa ttgatttata agtcattttg gggctttttt atgtaaatat
     6361 aatatgcaaa tatagtttgt ttaaaaatat ctattttaat tatattctta aatttattat
     6421 tggatacagg cattcattaa ttgtttgatg aatagaaaaa tgttttgaag gtgaaaatag
     6481 tattgaaaaa ataatatact aagggtataa ttcatttgat ttcatttgta taaatttaat
     6541 tataaatttt aaagtgatta tataaattta aatgctaatt acttaataaa ttttaataat
     6601 aattatttaa taaataatta ttataatatg ctagaataaa tatatataaa taagttttga
     6661 tgaggtacta ctataaaggg gggactttat gttttcgata ccagaagtaa aaaaagtaga
     6721 agaggtaatg gatacaaaat ttacaactat agacgaagac acaagaattg aagatgcaat
     6781 aaaagaaatg ataaaaagca acacaaaaac tctaatggta atagattcat ctgaccaatt
     6841 aaaaggcatt atatccatga cggatataca taatctttat gagatgcata aaaagtatga
     6901 aggtcaacca gtaaaactta taatgaagaa ggatgtaata tatgtcaacg aaggtttaac
     6961 cttagatgaa tgtagagata taatgatatt aaaaaatata gggattcttc ctgttcttag
     7021 agataataaa attattggag ttctaaaaca agagcacatt agggactatc tatatatgca
     7081 tttagaagat tatggattaa ctttgaagta tattataggt caaattaaag aaggaatttg
     7141 tgctataaat aacgaaggtg ttgttatctt gtggaataag tttatggagg aaaggtatga
     7201 tataaagtct gaagatatag ttggtcgacc gatgaatgaa tttttggaaa atactatttc
     7261 agaaaaagta ctaaatagta aagttggaat gagtgacttg tattttacag ataaaaaaga
     7321 aaatatgtat gcattagttc atgcaaatcc tatattttat aaagaagaat ttatcggtgt
     7381 tgtatgtaca gaagttgatg ttacagaagc taagatatta gctcttgaac ttgaaaaagt
     7441 aaatgataca ttaaaatatc taaagaacga agtcaaaaat ttatctaaag gtagttttga
     7501 taagatttta ggtaaaagtt ataaattgga aaaatcgaaa gcgattgcaa aacaagtggc
     7561 tagaacaaat agtagtatat ttatttgggg cgaaagtggt acaggtaaag aagtatttgc
     7621 aagagctatt catgattata gtgagagaaa agggcaattt atacctgtta actgtagtgc
     7681 cataccaaat gagttatttg aaagtgagtt ttttgggtat gaatcaggtg catttacagg
     7741 agcaagtaaa aagggtcgaa taggtatatt tgaacttgca aaagatggaa ctgtattttt
     7801 agatgaaatt gccgatttac ctcttagtat gcaagcaaag ttgcttagag tattacagga
     7861 aaaagaaata agaagagttg gtggagatac cacaataaaa ataaatccta gaataatatc
     7921 agctaccaat aaagatttga gtaaaatggt aaaggcagaa aaatttagag aagatttata
     7981 ctatagatta aatgttgttg aaataaagat tcctccactt agagagagaa aagaagacat
     8041 aggattattg gttcatagct ttttagagga gatatgtaaa caaaataata agcctgttct
     8101 tacaattagc aaagatgtaa tagatatatt tcagaattat agatggaaag gtaatattag
     8161 agaattaaaa aatacgattg aaaatattgt tgtattgtca caaaattcta aaatagatgt
     8221 ggatgatgta ccaagttata tgatggattc tacaaataat agtactgaag aagaagagta
     8281 tcctttagat ttaacgaagg caactcaaaa aattgaaata aagaatataa ccaaggcact
     8341 taaaatgtct aatggaaata aagctaaagc agctaagata ttaaatatac ccagaacaac
     8401 tttgtattat aagatagacc aatacaaaat agatgtgtct aaaatatgac acatgactaa
     8461 attttgacaa aatgataaac tttaatttaa aacctagtaa agccaacact tgtataagtg
     8521 ttgtgtatat agatgtgtta aaaaaaatga aaaagtgttg attaattgac actttttttg
     8581 tttgagaaaa tgtttacaaa tacaaaaaat aacatatttt aaataaatat ttatataata
     8641 tttggctaaa aatagcgata gatgaagaat ctattataga aaaaatcttt ttgttaataa
     8701 gttggcatag gaattgctta taatataaag tgactttaag tgagaaaaaa ataatattaa
     8761 tcataacaaa ttatgtaggt tttaatttac ttaatttctc agttaagggg ttacctgttg
     8821 aggaattgag atttaaaata ataaataaga aaataatgta aggaggtatt ttatactatg
     8881 tcaataactt tagaaacagc tcaagcccat gcaaatgacc cagcagtttg ttgttgtaga
     8941 tttgaagcgg gaacaattat agcgccagaa aacttagaag atccagcaat atttgcagac
     9001 ttagaggatt ctggattatt aacaatacca gaaaatggat taactatagg tcaagtacta
     9061 ggagctaagt taaaagaaac tttagatgca ctttctccaa tgactacaga taacgtagaa
     9121 ggatacaaag caggagaggc taaagaagaa gtagtagaag aaacagtaga agaagcagct
     9181 ccagtatcag aagcagcagt agttccagta agcacaggag ttgcaggtga aacagttaaa
     9241 atacacatag gtgaaggtaa gaacataagc ttagagatac ctttatcagt agctggtcaa
     9301 gcaggagttg ctgctccagt agcaaacgtt gctgctccag tggcaagtgc agcagcagaa
     9361 gtagctccaa aagttgaaga aaagaaactt ttaagaagct taactaaaaa acactttaaa
     9421 atagataaag ttgaatttgc tgatgaaact aaaatagaag gaactacttt atacatcaga
     9481 aacgcagaag aaatatgtaa agaagctaat gaaactcaag agttagttgt agatatgaag
     9541 ttagaaataa taactcctga taaatatgaa acttacagtg aagctgtatt agatatacaa
     9601 ccaatcgcta ctaaagaaga aggcgaatta ggttcaggta taactagagt tatagatgga
     9661 gctgtaatgg tattaactgg tacagatgaa gatggagttc aaataggtga atttggttct
     9721 tcagaaggtg agttaaatac tactataatg tggggtagac caggtgctgc tgacaaaggt
     9781 gaaatattca tcaaaggtca agtaacaata aaagcaggaa ctaacatgga aagaccagga
     9841 cctttagctg ctcaccgtgc atttgactat gtaactcaag aaataagaga agcattaaag
     9901 aaagttgaca actctttagt agttgatgaa gaagtaatag agcaatacag aagagaaggt
     9961 aaaaagaaag ttgttgttat aaaagaaata atgggacaag gtgcaatgca tgataaccta
    10021 atattaccag ttgagccagt tggtacatta ggagctcaac caaacgttga cttaggaaac
    10081 atgccagttg tattatctcc acttgaagta ttagatggtg gtatccatgc attaacttgt
    10141 ataggacctg catcaaaaga aatgtcaaga cattactgga gagagccatt agtaataaga
    10201 gctatggaag acgaagaaat agatttagta ggtgttgtat ttgttggttc tccacaagta
    10261 aatgctgaga aattctatgt atctaagaga ttaggtatgt tagttgaagc tatggaagtt
    10321 gatggagctg tagtaactac tgaaggtttc ggaaacaacc atatagattt cgcatctcac
    10381 atagagcaaa taggtatgag aggtatacca gtagttggtg taagtttctc agctgttcaa
    10441 ggtgctctag ttgttggtaa taaatacatg actcacatgg tagacaacaa taagtctaag
    10501 caaggtatag agaatgaaat attatctaac aacactttag ctccagaaga tgctgttaga
    10561 ataatggcta tgcttaaaaa tgctatagaa ggtgtagaag ttaaagctcc tgaaagaaaa
    10621 tggaatccaa atgttaaatt aaataacata gaagctatag aaaaagttac aggagaaaaa
    10681 atagtattag aagagaatga gcaatctcta ccaatgagta agaagagaag agaaatatac
    10741 gaaaaagacg aaaactaaaa tttagatact aactaagatt tagacaataa cataaattta
    10801 gatccaagtt taaaacagta tagagaggta gaattataat ggataggtta aaatatatct
    10861 catcagagac tttttatgaa ggtattatag ttgacatcaa aggcggtgga gtgactatag
    10921 acctaaaagg tagacttgga caatttaaaa tacctaatag aatgcttata actgactacg
    10981 aacttaagat aggtcaagaa gttggattca tgctaagcta tcctgaggta ttaagtccag
    11041 agcctaatca agagtatgta gaaaatatta ggagagaaaa agaaaaacaa gcagaaatgc
    11101 aatcaaaaca agaagaaaaa taataaataa tcgtttgaaa atagaaggga gaggaaatat
    11161 atgagcctta caacagtaca aggacttcaa tctgaaatat tcgttccaat aacacctcct
    11221 ccagtttgga ctcctgtaac taaagaatta aaagatatga ctatagcttt agctacagca
    11281 gctggtgttc atttaaaatc tgacaagaga tttaatctag caggagatac tacatttaga
    11341 gctataccta atacagctac agttgatgaa atgatggtat cacatggtgg atatgacaat
    11401 ggagatgtaa acaaagatat aaactgtatg ttccctatag atagattaca tgaattagct
    11461 gcagaaggat ttatcaaagg agtagctcct atgcactatg cattcatggg tggtggagga
    11521 aaccaacatg tcttcactga agaaactggt cctgctatcg ctgctaaact taaggaagag
    11581 ggagtagacg gtgtagttat gacagctggc tgaggtactt gccatagaac tgccgtgatc
    11641 gtgcagagag caatagagga agctggaata cctacaataa taatagcagc tcttcctcca
    11701 gtagttagac aaaacggaac tcctagagca gttgctccat tagttccaat gggtgctaat
    11761 gctggtgaac cacataatat agaaatgcaa acacatatat taagagatac attagagcaa
    11821 ttagttgcaa taccatctgc tggtaagata gttccattac catacgaata taaagctcac
    11881 gtttaaaatg taatttatat ttgaaaatat agaacccttc tcctacacgg ttaggagagg
    11941 ggttttaaga acaaaggtgg atattatgga agaaaaaata atgagaagac ttgttataaa
    12001 accgtttcat atgaatgaag ttaatttcgg ttcaaaaact tctataaaga aagatgtact
    12061 tacaatagat ttatcaagta tagatgaaat aaaagaaaga gaagagttaa taacagacat
    12121 aaaagtagac attataaaac ctggagatta tgacagagaa attaatacta ttatggacat
    12181 tataccaata tcaacaaaag ttttaggcag actgggagag ggtattacac atactttaac
    12241 gggtgtatat gtaatgttaa ctggtgcaga tgaagatgga aatcaaatgc atgaatttgg
    12301 ttcttcagaa ggaattttga aagaccaaat ggtatttggg agatatggaa ctccatcagt
    12361 tgaagattat ataatccatg tagatgtaac tttaaaggga ggtttacctt ttgaaagaac
    12421 acttccactt gcagcattca gagcatgtga tgattttata caagaaataa gagcgtcttt
    12481 gaaaatggaa gacggaagaa atgctactca agttcgtgaa tattttgaca aaattcgacc
    12541 aaatgcaaaa aaggttgtca tagtaaaaca aatagcaggt caaggtgcta tgtatgacaa
    12601 tcagttattc tcaaaggaac ctagtggatt tgaaggagga acatcaatta tagatatggg
    12661 aaatgtacct atgattatat ctcctaatga atacagagat ggtgccctta gagctatgac
    12721 ttaagagagg tggatattat gggaatagga ccttcaacaa aagaaacatc tcttcatcac
    12781 tttagagacc cacttttaga catagttgaa agtgataagg atgtagattt acttggagta
    12841 atagtagtag gaactccaga tggaaatgaa aataaaacct ttgtgggaca aagaacggcc
    12901 gcttggctgg aagccatgag agtagatgga gctatagttt catcagatgg ttggggaaac
    12961 tcacatgttg actacgctaa cacatttgag gaaataggaa aaagagatat acctgtagtt
    13021 ggagttacat ttaatgggac tcaagctaag tttgttgtaa gcaatcaata tatggatacc
    13081 atagttgata tgaacaaatc aaaagaaggt attgagacag aagtagttgg agaaaacaac
    13141 acaaatgaaa tagatgctaa aaaagctttg gcatttttaa aacttaaaat gagaaaacat
    13201 ggataaaaaa tttaagtaag ggaatgagtt ttgtgggaat ttctacatca atgaaaaaca
    13261 catcacttca tatatttaga gacccgttac tagatttgat agataatgat tctgacatag
    13321 acttgttagg agtggttgta gttgggacat cagagcacaa tgaatggaaa acatttttag
    13381 caactagact tggaagatgg attgaatcat tgcgacctga tggagtgatt ataactttag
    13441 atatggcagg aaaccagcac attgatttta caaatgctat agcagagttt gtaaaatcag
    13501 atatacctac agtaggtctt actattatgg gagcagatgg attagtaatt accaatccat
    13561 agttggataa agctaccata attgattata aaaagacaac aggctacata gaaacagaag
    13621 tagttggaga caatcacatg gatgaagttg atgttaaaaa agcattggct ttcctaaaat
    13681 taaaaatgcg taaagacgca aaataaacct aagggggaat ttgatatgaa atttagcaga
    13741 agtatacaag ctatagactc tcatacagca ggtgaagcaa ctagaatagt tgtaggtgga
    13801 atacctaaca taaaaggaaa ttctatgcct gagaaaaaag aatatttaga ggaaaatcta
    13861 gattatttaa gaactgctat aatgttagag ccaagaggac ataatgatat gtttggttct
    13921 gtaatgactc aaccttgttg cccagatgct gactttggaa taatcttcat ggatggtggc
    13981 ggatacctta acatgtgtgg ccatggtaca ataggagcta tgacagcagc tatagaaaca
    14041 ggtgtagttc cagcagtaga gcctgtaact catgtagtta tggaagctcc agcaggaata
    14101 ataagaggag acgttacagt tgtagatgga aaagctaaag aagtatcatt cttaaacgta
    14161 ccagctttct tatataaaga aggtgttgaa gttgacttac caggtgttgg aactgttaaa
    14221 tttgacatat catttggtgg aagcttcttt gctataatac atgcaagtca attaggttta
    14281 aaaatcgaac ctcaaaatgc tggtaaatta actgaactag ctatgaaact tagagatata
    14341 ataaatgaga aaatagaaat acaacatcca actttagctc atataaaaac tgtagactta
    14401 gttgaaatat atgatgagcc aactcatcca gaagctactt acaaaaatgt agttatattt
    14461 ggtcaaggtc aagttgacag atctccatgt ggaactggaa caagtgctaa attagctact
    14521 ttacatgcta aaggtgaatt aaaagtagga gaaaaattcg tatatgaaag tatattagga
    14581 actttattca aaggtgaaat agttgaagaa actaaagttg cagatttcaa tgctgtagta
    14641 cctaaaataa ctggttctgc ttatataact ggatttaatc attttgtaat agatgaagaa
    14701 gacccactta aacatggatt tattcttaaa taagtaataa ataaattagg aaattaaaat
    14761 agaatagtag ccacttgtcg gttgctattc tatagctttt aggctataag attcagaata
    14821 tatgaaaaaa taaaaaaata ggaggatgga ttatggtagc aatcttaaag gcattatatg
    14881 tagtattcgc agcaatcttt gcagttgtgt ttggtaaaga cgtagctaaa gcaaacaaag
    14941 aggggaaact agaagaacaa agtactccaa aagttgctgt aacaggattt ataacagact
    15001 tctttgatac attaggaata gggtcatttg ctccaacaat agcaatgtca aaggctttaa
    15061 agttaaatat accagataaa aaaatgcctg gtacattaaa cgtagcacat actattccag
    15121 ttgttacaga agcattcata tttacatcaa tcataccagt tgatggtgtt actttagtat
    15181 ctatggttgt ggcagcagca gttggttcat atataggtgc aggaataata gctaagatgg
    15241 acgagagaaa aatacaactt gttatgggtg taactttagc attaacagca atattaatgt
    15301 tattaggtca tccttggatt aacgtattac caggtggagg aaatgaatta ggtcttactg
    15361 gtgttaaatt agtaataggt gtaataggta actttatact aggagcttta atgacagctg
    15421 gtataggttt atatgctcca ggtatggcaa tggtttattt cttaggaatg tctgcaaaag
    15481 ttgcattccc aatagttatg ggttcttgtg cattattaat gccagttgca agtatgaaat
    15541 tcataaaaga agatgcatat actaaaaaag catcagttat aatagctata tgtggtttaa
    15601 taggagtttt cgtagctgca tatatagtaa aaactctacc aatggatata ttaaaagcat
    15661 tagtaatagt agttatagct tatacttctg ctacaatgat aatggctgca aataaaaaca
    15721 aaaagactgc ttagttaaag actacttagt gtttattaaa tattacagct aataataaag
    15781 attataaact gctttaaaat attgaattaa tattttaggg cagtttttta tttactaaat
    15841 taatataaag caattaacat gccaattatg taatatatat agtgatttag tagatttaat
    15901 gtaaaaaacg ttgtattttt aaagaataaa gcataattta aagctaaata tttaaatttt
    15961 ataatcaata agttcaataa atgttcatat attaatacag attaataaaa atataaccta
    16021 taataagtct aatataactt ataattaaga aaaataagtt tataatgact caatattcat
    16081 ctgaattatt tgaataaatt taattcgttg aagttgctta tatagagtga ataaa
//
LOCUS       DACULQ010000089        15897 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.089, whole genome shotgun sequence.
ACCESSION   DACULQ010000089 DACULQ010000000
VERSION     DACULQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.089"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(121..2187)
                     /locus_tag="JA154_11080"
     CDS             complement(121..2187)
                     /locus_tag="JA154_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAT7996405.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(2264..5299)
                     /locus_tag="JA154_11085"
     CDS             complement(2264..5299)
                     /locus_tag="JA154_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HAT7996406.1"
                     /translation="MGMENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKK
                     LNDNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGN
                     NEEINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDL
                     DPKFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAA
                     IDNPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELL
                     PYSEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYK
                     IKNKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVII
                     SIAISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIE
                     LLSANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYM
                     EKNKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDV
                     TKEFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYI
                     NDTYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSV
                     KKQMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVS
                     EFDINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFS
                     SPIDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFE
                     EIEDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLL
                     YIMPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINL
                     GVDYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(5346..6806)
                     /locus_tag="JA154_11090"
     CDS             complement(5346..6806)
                     /locus_tag="JA154_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7996407.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(6898..7272)
                     /locus_tag="JA154_11095"
     CDS             complement(6898..7272)
                     /locus_tag="JA154_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996408.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(7448..8194)
                     /locus_tag="JA154_11100"
     CDS             complement(7448..8194)
                     /locus_tag="JA154_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein MotB"
                     /protein_id="HAT7996409.1"
                     /translation="MFMLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAE
                     KYKQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLN
                     ENGMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRV
                     EGHTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENS
                     SEEGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(8209..8997)
                     /locus_tag="JA154_11105"
     CDS             complement(8209..8997)
                     /locus_tag="JA154_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HAT7996410.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLTFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(9085..9175)
                     /locus_tag="JA154_11110"
     tRNA            complement(9085..9175)
                     /locus_tag="JA154_11110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9139..9141),aa:Ser,seq:gct)
     gene            9434..10198
                     /locus_tag="JA154_11115"
     CDS             9434..10198
                     /locus_tag="JA154_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAT7996411.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(10479..11594)
                     /locus_tag="JA154_11120"
     CDS             complement(10479..11594)
                     /locus_tag="JA154_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087219.1"
                     /note="may be involved in the transport of ethanolamine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAT7996412.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(11635..13161)
                     /locus_tag="JA154_11125"
     CDS             complement(11635..13161)
                     /locus_tag="JA154_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HAT7996413.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            13454..14551
                     /locus_tag="JA154_11130"
     CDS             13454..14551
                     /locus_tag="JA154_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HAT7996414.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(15004..15516)
                     /locus_tag="JA154_11135"
     CDS             complement(15004..15516)
                     /locus_tag="JA154_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996415.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KMDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT         5149 a         2789 c         1680 g         6279 t
ORIGIN      
        1 ataatatatt aaacccttat ttctttttct aataagttaa ataatacatt ttacacatat
       61 ctatttgtct tgcacataat aaataataca ttatatttct atctaaattt agattctaaa
      121 ttaaatagta taaattatat tcttcaataa acaagttttc aaatccaaga ttcgctctct
      181 atctttaaac tttattgtaa acataattcc atctttttta attatttctc cttcatgaaa
      241 ttttttatgg tataccttat cccctacacc aatactgtca atttcttttt tcgttggaga
      301 ttttatatca ttaataaata atgatttatc aactttctta ccatacttat ttaaagtaga
      361 gcttatacat aacttttctt cagctcttgt aatagcaaca tacataagtc ttctttcttc
      421 ttctaattgt tcttcttttt tctcttcctt acaaatatca tatgattttt catgaggtat
      481 tgttccttca tttacaccta taatataaac atttctaaat tctaatcctt ttgcactatg
      541 catagttgta aatatgacac cctcagtctg cttattcttg ttattttccg ttaactcagt
      601 ctttactcta tcaatatgct ctaaaaactc tgtaacagta ttaaaattag ttgctgaact
      661 ctcaagttca tttaatatct ctacaagtcc attagtcttt atctttctgt tgctacaata
      721 atctaaaaca tatctatcat aatcaagact agttctaata taagatattg catttttagg
      781 atttaaagta tttaaataac ttaaatctat ttccaaatca tttattgttt taacttgttt
      841 aggatgaaga ttacacttat ttattaatgc tgttataaag tctttttcat ccttaaccat
      901 atttatactg tctttggata tatatctaaa aggcttgttt ataattctca accaatcttt
      961 atttgatttt ggattaatac ctattcttaa ataggctagt atatcttgac ttgcccaatg
     1021 gtcatatatt gtaattactg agtcttttac tacaaatgga atcctcatat ccataaatac
     1081 atctactaat gctcttgact gtatattagt tctataaatt actgcaaaat ctgaatactc
     1141 tacataatct ttttgtattt cgtcaattat ttccctggca atatataagg cttcttcctc
     1201 tgaatcatgt ggagaaatat aggtaacact tcctccatcc ccctgactac acttaatgat
     1261 tttttcatat ctatttttat ttttgtcgat aagtctattt gcagtatgca ctatctcact
     1321 tttagacctg taatttatat ctaatattat ttttttagta ttattaaagt attgttcaaa
     1381 ttcaagcaaa aaatctggtc tagcacctct aaatccatat atactttggt cttcatctcc
     1441 aactgcaaat atattgttta atgggctaca aattaacttt aaaacttcaa actgtacttt
     1501 atttatatct tgaaattcat ctatcaatat atatttatac acatttctaa ccatttctag
     1561 tgcagactta ttgttcaaaa gtaaataata agttcttata agcatatcat caaaatctat
     1621 tttattaact ttagattttt gttcttcgta taagttgtat acttttaaaa actcatcttt
     1681 tgttaatact tctgaattaa attcattttt atccataagt tcatttttta cataggaaat
     1741 ttcatttata acttgtccta tattctcatc atcatcagca ttctctatat ttaaactttt
     1801 taggattgcc ttgattgtca ttcgctttga cttttcatca aatatactgt ctaagttgta
     1861 tctttcaaag tatcttagaa ttttaaaaaa tacagagtga aaagtaccat aggttacctt
     1921 attaagtctt atgtcatcac ttaaattcaa agctctattt ttcatttcta ttgaagatgc
     1981 cttggtaaaa cttattgcca agattcttgt tggtgctatg tttttgttta caaccatatt
     2041 ggctattcta tatgtaatta ctctggtctt acctgaacca ggccctgcaa gtatcatgca
     2101 aggcccatct atatgctcaa ctgcttctaa ttgattttca tttaactttc ttatatctat
     2161 atttgttgtg attattagct ccaccacctt gatttataat tatattattt tctttttaat
     2221 tataaactaa aagttcataa aaataaattt aatttttcct ctattaaacc acatcattcc
     2281 tattttcatg acatatcttg agttcttttt taattttgtc gcttatatct tcaggtataa
     2341 atgatggttt actaatatag tagccttgca tataatctac tccaagatta attagtgttt
     2401 ccatttcacc ttttgtttct acaccttctg ctatcacttt aatatttctt tcgttagaat
     2461 atgagataag attttttaat attttctgtc tgttttcatc tttatcaata cctcttacaa
     2521 tagacatatc aatctttaca tagtctggca ttatatataa tagtacagca tctccgttgt
     2581 aacctgtccc aaaatcatct aaagcaattt ctatattcca atggtctata tatgctttct
     2641 ttttttcaat aaaatcttca ttaatcttat cattctctgt tatttctagt actactctat
     2701 ttaaatattc accatataaa tcttcaattt cctcaaaatc tacttttgac aaattattat
     2761 taggaataga atttaagaac aatttgcaat cgccaaattt ttctttgtat tttttaaatg
     2821 atttaagtgc tttaaaccat gtaatacgct caatttgata taatttagac tgtgaacgag
     2881 ctaatctaat gacatctatt ggtgaactaa aagtttttat tttaggtctc ataagtgctt
     2941 catatgcaaa tactttacct ttacaatcta caataggctg aaatgcatac tcaactaatt
     3001 gaccatcaat taacttgttt aattcttctc tattactcaa taagaatgaa tccttattat
     3061 atatattaat atcaaattca ctaacttcac ccttgattgt atttttaatt tgatacatag
     3121 caaaatctgc gtatctcatt aattcatcat agttggttga gtcatcaggg taccaggaaa
     3181 ttcctgctga tgcccttatt ttaaattctg ttccatcatg aagttttaat aaggtattat
     3241 tcatctgttt cttaacagaa ttaataatat ctcttatact atctttgctt tcatatccat
     3301 atataaatac ataaaactca tcacctgaaa gtctagatac tataccattc catgatataa
     3361 acttcttaag tatgtctgca gcacacctaa tatattcatc accagcatca tgtccatatg
     3421 tatcattaat atattttaga ttatctaaat cccacataat aaatgctgct atcttaagtt
     3481 catctttatt cttaaatttt tcttgtatct gtgaatgaaa agctctccta tttaaaagat
     3541 tcgttaaaat atcaaaatct ctttcatatt ctatcttcct tttctcaata aattcttttg
     3601 tgacatcaac aacaacacca agaaccttta gctcatcttc aaagatattc attcttatcc
     3661 acttaagttt tttatcttta gattcaaact tatatatata tgtattttct gtttcataac
     3721 tttcttgcaa atatttatct agtcccctta attttttcag gaacttattt ttttccatat
     3781 atccatattc ttcaaatttg atttcagtag taaacacgct aaaaaattct tttgtacaaa
     3841 aaacattctc tgcgtttttg tataactcaa aagccccaat tggcatatct accatttcaa
     3901 taatctgtga taattttgac gaagaatctg caacatttgc actgagcaac tctatagctg
     3961 aagataattc atctatttca gatatattta tcttatcaag atttactggt tttgatggat
     4021 tactgtttct aactttgttg gctaaaagaa caattggttt tgtaaataat cttgatgcaa
     4081 tgtaaatacc tattagacct attaataaag aaattgctat agatattata actaatttct
     4141 ctaccctttt tgaaaatgac aaaagacttt tttcttcaat aatacccaaa agagcccaac
     4201 gttcattttc aaatggagta ttggagttat ataaatccaa gtattgtaca ctagcataaa
     4261 tgttgccatt accacattca ttatctttat ttttaatctt atatatattt ttatacgatt
     4321 ccttctgctc taaattagaa tgtaaaccat cccaaaattt ttcttttaat gagtatccat
     4381 ttgaaattac attttcaaag gccatatcat tatttctatc tacacctaaa atataattac
     4441 cctttttatt tccatttatc tctgagtatg gaagtaattc tcttaaataa tttacactta
     4501 aatctattcc aagtactcca tatacagttc catcttcata aataagggga actgtatatg
     4561 tcataagcct atcacctgca tttgtcaata aaaacttact tccccagtat cctaaattag
     4621 aacttgatac atttggatta tcaattgcag ctctaaatgg tttataaaaa aaatcatctc
     4681 catcttgctc attttcactt ttaaatgaaa acttaggttc ccaataactg tccatagata
     4741 ttccaaaact tcttgctata tctgatgaac ctctctcaat taacaaatca gaattatcat
     4801 ttggattaaa cttagggtct aaatccctta tatacaatcc tgtcctactt atttcacctt
     4861 tactatctat tgacttatcg ttaatatttt tgtcacttag gattataaaa gctcctgtaa
     4921 cagaattttt tctcaaaaga tatattaaat catttgatat gctttttata atatctttgt
     4981 taatttcttc attgttgcct acatcctcaa ttaaggcatt tttttcattt aatacttttt
     5041 ttacagagga attaattgta gcttccgttt cacttgtatt tgaccatctt tgtaacattt
     5101 cattttgtat ataattttta cgattaatta ctctttcatt taagatgtca aacgaattat
     5161 catttaattt cttaatagtt cctccccata aaattgtggc acaaaataaa cttgtttgaa
     5221 ttagcataac aataattagg ggaaatatca tctttttcat tattgaattg ttttttctta
     5281 gattgttttc catacccatt ccacctatac ctttgtgctc gtcaacacac taactgccaa
     5341 ttcatctatt tttttatagc attttcaagc tcagccttaa aactttcaaa ccaagtttta
     5401 aaatttttat cattattaaa ttgtgctact gcatccttct tagaagttcc actttttact
     5461 aattcattta ttttatttga atcttcttta gctttatcaa gcattgaata ttcaagtatt
     5521 tgtctagctt tagttccacc atcaaatgct ttattagtat ataaattaca actgttaatc
     5581 tgttcaatag atgaggctat tgaaccttca actttatcag ataaactcaa attatctttc
     5641 ttcataactt cttttataac atccaaatca ttcgtttttt tctttactgg taagtatcca
     5701 gaagcaatag agaattttat attaggttct ttatctgtaa accattttaa aaattctact
     5761 gatgcatatt cttcttcttt agtagatttt gttacagcca ttcctgcacc ttgttgtaca
     5821 gcatattttc cttctccttt aaaacgtggt gcttgaaaaa cagatgtttc aatattataa
     5881 ctctcattat catttagcat tactctatct ggaaagtatg ctgctcctga actagaacct
     5941 acaagagcta atatatcacc tgtttttgca tcatcagaac gaaacttacc ataagaagca
     6001 aaatatccat ttatatatgg tacatagaaa ttatcccata gttttttcat aactttttca
     6061 tcaatattta gtgtaacttt accatcttta acagaaaaaa tttctactcc tagttgttta
     6121 cttcctataa taatataatt tgctaaagca tctcttccaa aaaatgcttt accatcatta
     6181 ggctctttcg ttaaactatc tgtccattca taatattttt ttgatgtctc tactaatcct
     6241 tcaattgttt ttatatcatt tatatttgaa tttgtagctt ttgcaaattt atcccaatca
     6301 gttttattta acatcaaaat ttcagttgat tttgcaattg ggaaaagttt aaactcatcc
     6361 ttagaattta ttctgccttc ttctacatat tcgtctatgt attcatttaa ttcttcttta
     6421 cttatgtact tatccatatc tactagtaac cctaatttat ccatttcata tgcagtatct
     6481 gagtatgctg caaatatatt tggtacatct tcagaaccaa cttttttatt tgcagaatct
     6541 aaaactttct ctgataaatc aactacatta ccttgactga atgcttcaac aataattcct
     6601 ttttcagcac ctacagtgtc attaaattcc ttaacaagct tatcaaaagc cattttctgc
     6661 tgtccattat aataatgcca aatttgtatt gatgtcggat tttctgggtc taacttataa
     6721 ttatcttcct tctttaaact acatcctgta agtaataaaa caatcattga tgtgataatt
     6781 aattttgcaa acttacacct attcataaaa tctactcctt caaattataa aatttataat
     6841 agcattaaaa ctgtaacttc actatttata ttaattatag aacgtttatt gatatattta
     6901 aacaggattt acaacatatt taaaagcaat taagaaacat actattacaa atatagaaaa
     6961 taaagctttt acataatttt tatttctaaa tgcaccaaat gacaccaatc cttgttgaat
     7021 agctactgta attactaaaa gtatcattat tatattaagt tgaaccagtt taaataagta
     7081 aagcaatgaa aacactacta gaagtacaca taatacaata ctagatttat taataatatc
     7141 atcaatctta tctactttct tagattgctt attaatttta tttaattttt ctttatttaa
     7201 attctcaact tttttattat acctttctct tgttttatct ttatatttag tatttttatt
     7261 tttatttccc atttattttc catcctctat aaatttttta attgagctta tacattattt
     7321 aaatattata tatttaagat accatatatt cctacaaaaa ggtaacttac tatgtatcta
     7381 tttttgattt tcgcataaaa aagccatctc ctttagaatt aattctgtat tgagacagct
     7441 aatttaacta tttatccttt gaaacatttt ctacattatc atatttacta tcaactaaaa
     7501 ctatatcaac tcttctattt tttgacctac cttcttcaga tgaattttct gctattggtc
     7561 taaactcacc atatcctaca gcagataatt tatctggtgc tatacctgca ttgtcaataa
     7621 gaagttgaac aacattggtt gctctcataa ctgataaatc ccaatttgac ttaaactctg
     7681 aattttttat agacatattg tctgtatggc cttctactct catgtaacta ttcatttcat
     7741 ttaacatctt acctatttga ataatcttat ctctagaatt atccttcaca atagctttcc
     7801 ctgtatcaaa tagtattgta tctttaaggc ttattactaa tcctctatct tgaactctta
     7861 atgatactga attagccata ccattttcgt ttaaatactt ctgaatttct tcaccaattt
     7921 ttttaaactt agcattttgt aaagtatcta aatcattact tcctggttca actactggtt
     7981 ctatcttgct attaccacct tcaataactc cagaagaacc accaaaagca gaatttagag
     8041 attgagataa ttgtttatat ttctccgcat caacattact catagaatac attattacaa
     8101 agaaaatcat aagtaatgtt atgaggtctg aataagttaa aagccatcgc tcattgtttt
     8161 cttctttttc atcttcatca tacaacataa acattccatc cttttatatt attcagctct
     8221 accaccaatt tcttcaagtt caattagttc ttttgaattt aaaaatattt ttaatttttc
     8281 tgtcattaaa ttagaattat caccctcaag tatggataat attgcttcta tcaccaaaag
     8341 tctttcactt acttcttgtt gatttatagc cttaagtttt gatgctattg gtaaccatat
     8401 taaattggca gaaccaacac catacaaagt agccaaaaat gcaacagaaa tttgaggtcc
     8461 taatgcatct ggattacttg aaagattacc taaaatatgt actaatccca taactgtacc
     8521 tattatcccc agtgtaggtg catatccacc tgctgaatca aaaattgcta ttccttcttt
     8581 atgtcgtttt gatgttatct gtatctgtga ttccaatata tttcttatag taactgcatc
     8641 cactccatct accataagtt caagcccttt tctaataaaa gggtctaatt catcattctc
     8701 acttatttct ttgtcaatac taagaagccc ctctttccta gcctttgttg caatttcttt
     8761 gaaatagatt atattttcta tgttattagg ctttttattt ttaaaagtta ataacattat
     8821 ctttatagac tttttaaaca cagggaatgg agtcgaagct ccaacagctc ctatagttcc
     8881 accaaacaca acaagtgctg atgaaaatac aaataatgct ccaacatgtc ccccatcaat
     8941 aacaaaagca actaccaaag atgctatccc cactatgaaa aaaattaaag ttgtcatcta
     9001 ttccctcctt tctttagtta tacatatcta atcatttatg acattataaa aagactatct
     9061 ttaataaaaa tagcctttaa taattggcgg agaaggaggg attcgaaccc tcgcaccggt
     9121 tacccagcct aatcccttag caggggatcc tcttgagcca cttgagtact tctccatatc
     9181 tctatactag taaattatat aataaaatca tattttagtc aattgtttta taaaatttga
     9241 gttattttga attattaaac atctattttt acttttttgc ttaaatgtaa gttttaaata
     9301 tggtaaaatt atcttgcata acacttataa tattatttga taaatataca tacattaaca
     9361 aatataccta aactctgact taattgtaag ttatgccaaa actaaaataa tactagtcca
     9421 aggaggatat tttatgttac cacaggaaag atatgagaag attttaagta ttctcaaatc
     9481 agataatata attaaaataa atgacatagt tcaaatgttt gatatatcaa tagaaactgc
     9541 acgaagagat ttagcccata tggagagtat cggtctttta aaaagagttt atggtggtgc
     9601 tataccagtt atagcaagta gcgtagaact taactatgtt aaaagaaaaa aaataaactc
     9661 aaacgataaa aaacagattg ctattaaatg tagtgagttt ataaatacaa atgatactat
     9721 ttttatggat actggaacta ctgtacttga agtttcaaaa aatttgaaag ataaaaagaa
     9781 tctaactgta ataaccaact caattttagt tgcaaacgag cttatgaata ctaatataaa
     9841 agtatatgtg cttgggggct atcttagaga aactgaaggt tctttttctg gtccgcttac
     9901 actgtcagct attgaacaat ttaatgttga taaagctatt ttaggcgctg gaggtatcac
     9961 tcttgaaaat ggaatctctg actatcactt agaagaagca cttgttagaa aaaaaatgat
    10021 tgaaaggtct agaaaatcta tagtagttgt cgaacctcct aagtttggta taaattcatt
    10081 tgcatcagta gctcctatag aacatataga tactattatt acctctgcta gtatagacca
    10141 taatataata atagagtttt ctcaaaaagg aataccttta gtcatagctg ataattagct
    10201 tatatgattg atttttagtt ccttgatgta ataaattaaa attaaggagc caaataatta
    10261 tttatttagc tccatttatg tctaaactat actttatatt ttaattatgt aactactttc
    10321 agttagctta tcatttttag ttatttgatt gccattttat ttaatttata agttttagtt
    10381 attcgattat tttttaattg tctgtctttt taatttattt ttagttattt aaactcctac
    10441 tttaaaataa tttaaatttg acttaagtat tgtttattct acactgcatc tttcttcatt
    10501 ttaatacttt gttcttcttc caaggttaaa tcttttgtca taagtatagc aagtgcaatt
    10561 gccaatactc ctccaaccaa ttttccaatt acaacaggtg taatcatctc tggaactact
    10621 cctgctgtaa atcctaaatg gtcccccaaa gctgctgtag cacaaaccag ccaagcagta
    10681 tttactactt tcccctttgg actcatatct ttcatcattt tatatactgg aatactattg
    10741 gctaaagtaa aaactaatcc tgctgcacta gtggcatcca tacctaattt tccacccact
    10801 attgtaagtg gtttattaag aactttaact aataaatgta atattggaaa agtacctaat
    10861 aacacgattg caatacttcc aattacttga ataccttctc ttataggtgt catgcctttt
    10921 ataagtacta tacctgttat ttcttgaaat gctgctgctc ccaaacctat cgttatcaat
    10981 atagatataa attttccaaa aactaatgac ccatttatca taagcgtaga attaaatttt
    11041 aaacctactg ccaatattac tgccaatatt atcactggta tcatatttat aattaccatt
    11101 gataagttaa atcctgctat taatcctcct ataatacctc caaaaggtat tgttatcaag
    11161 cctatcagta acccttttgc aaagaatggt ctgtcttcat attctataag acctagtcct
    11221 actggaattg aaaatactaa tgtacatcca agcatggatg acacaaccaa tcctgcaagc
    11281 attcctgcct ctttattgtg tgcaagttcc atagccaatg gataccctcc catatcattc
    11341 gctaaaatag ttgcaaacat agatgggtca gctccacaga gattgaagat tggtattatt
    11401 ataggaccta gtacatctga aattactgga gctaaacaaa ctataccaac cattcctaaa
    11461 gccaatggac ccattgaatt aaacccttct tcaaactgct cgccaagtcc aaatttattt
    11521 ccaattattc tatctaacgc tcctaaaagc actcccacag ccattatgta taatataatt
    11581 ttatcaaagg gcatttttaa ttcctcctat acatgtaaaa tactctattg taacttaatt
    11641 agcacacacc tttttggtat cgttttccca attcatagct ctttttactg cttggttcca
    11701 tccaagtaat atttcttctc ttttttgagt agtcatattt ggaatatatt ttttgcttat
    11761 tgtaaattca cttctaattt catcaatact gttccagaca ccacaagaaa gacctgccat
    11821 atatcctact ccaagtactg tcgtctcagg attttctgca atttcaattg gtatacctaa
    11881 tatgtcggat tgaaattgca ttgtaaagtt atttttagta attccaccat ctactctcaa
    11941 catttttatt tttttccctg aggctaagtt catagaatca acaacatctt tgacttgaag
    12001 tgcaacagac tcaagagtcg ctcttactat atgctctttt ttagtacttc ctgttattcc
    12061 aaccattgta cctctagcat attggtccca ataaggtgct gctatacctg caaatgctgg
    12121 tacaaaatat acacctccag tacttgatat ggaatttgcc atgtcatcag tttctgaata
    12181 attttttata actccaatgc catctctaag ccattgtatt gcagcacctg ctatatacac
    12241 tccaccatca taagcatatg taactttatc tcctattttc caagcaacag ttgtcaaaat
    12301 accattacct gctatattta gttcctcacc tatattcata tataggaaac atcctgtacc
    12361 ataagtattt ttaaccattc ctggttcaaa acatccattg ccaaataaag ctgctggttg
    12421 gtctaccatt gaagcagtaa tttttataga aaccccacaa aatgcctcct tatctgtagt
    12481 tccaaaatca ccactcgtag gcattatatc tgctaaaata cttctaggta ctcctataat
    12541 gtcaagcaac tctctatccc aatccatatc tcttatatta aaaagcattg ttcttgctgc
    12601 tgtagataca tcagttaaaa aagattttcc tcctgtcatt ttccatatta accaactatc
    12661 taatgttcct gcaagtactt ctccattttt aattttttct tttacacctt ttacattatc
    12721 tattacccat ttaatttttg tacctgagaa atatgggtct ataattaacc cagtcttatc
    12781 tattatctta ttttctaaac ctggtatatt ctttaaattt tccacataat cataagttct
    12841 cctgcactgc cacacaatag cattgtaaag tggtttacct gtatttttat cccataacat
    12901 tactgtttca ccttgattat ctataccaat tccttttata tcactttcat tgattccatt
    12961 ttcaaaagcc tgttgtaata cattattact cacagttttt gtattattcc aaatttctat
    13021 agggtcatgt tcaacatatc cagattgtgg atatatttga gtatgttcca tataatcgct
    13081 aaaaacaaac tctgattttt cgtttatcaa tactgctctt gttcctgttg ttccttggtc
    13141 aattgctaat acatatttca ctttaaatgt ccccctttat tttcagaatt aataaattct
    13201 tttttatttt ttgaatatat aatctattca agttatttat attttatatc atcatgtgga
    13261 tttatgcaat attcattaca atattttttg ttatttttaa ttatagtgat taaaaaagta
    13321 tttttattga aatattattg caatatttat tattttattc tataatctag ttaaaataac
    13381 acataatatt ttaatattta cacataatca gtcattcaaa aatttaaatt taacttttta
    13441 aggaggcttt acaatgaaaa aaacatatct attcagtcct ggaccagtaa tggttacaga
    13501 taaagtcaga aattcacttc ttcactatga tatatgccat agaggaaatg agtttatgga
    13561 tttattcaaa gatacacaac aaaaaatcaa taaactatat aatgcaactt ctaattacta
    13621 ctcattaatt gtatctggct ctggaacatc tgtaaatgaa tgtgtactat cttctatctt
    13681 tgacaaagaa gatgcagttc tgctagttag taatggtgtt tttggagaaa ggctagaaga
    13741 aatattatct acttataatg taaaaacata taaacctaat tttgaatggg cagaataccc
    13801 ggacctagac attttagaaa aatacataat tgaaaattct gatataaaag taattgctat
    13861 ggtgttccat gaaactagtt caggaatgat aaatccagtc cctgaactag gaaaacttgc
    13921 taagaagtat aacaaaatat tttttgtaga tgcaataagt gcctcagcag gtgaatatat
    13981 tgatgtggaa gagtttaata tagatataat aacaggtgtt ggtggcaaag ctcttggagc
    14041 atttccaggt tctgcatacc tttgtgctaa agagagtatt ttacaaaata ttaaagaaaa
    14101 tcaatgtaag aatgtgtatc ttaatcttta taaacactat aaagttgcta aatcatcttc
    14161 tcaaacacca aatacaccta atgttacttt aatatttgcc cttaatgaag ctttaactga
    14221 gtttttggaa gatgacagta aaatagaaag atacaaagaa tgttctgcta ttttaaggaa
    14281 tggtatggaa gaattgggtc taacattttt acttccagat aagtatatgt ctaatacagt
    14341 aacatctgta tttctaccta aagaaatcaa tgtaaatgag tttatattag ggctagaaag
    14401 ggaaagtggc tatgttgtct atccaggaaa aggtaaattc ctagatgcta atatattcca
    14461 agtagcaaat atgggagaaa tatatccaga agattgttat aaattcttag atatcttaaa
    14521 atcaaaattg gagataaaaa aagttatata acagtttgtt tgtaaaatcc tacaatatat
    14581 cagtataaat taaatagact gattttttta aattttccta taataaatta tattttatat
    14641 ttttgtataa gggtgaacta taatatgtaa agttcacccc tatttcaaat ctttttcctt
    14701 caatataatc tttttctttt aatttatttt atatttttga cataattatt cttacattta
    14761 attgtttttt atttttgacg caatcgtttt ttattcagtc attttatatt cttgatacaa
    14821 tcattcttac acttaattat tttttatttt tgacacaatc gttttttatt cagtcattta
    14881 atattttcaa cacaatcatt cttacattct attattgttt gttttgatac aactgttttt
    14941 attcagtcat tttatattct caacacaatt accttttcat tcaatcaaag taatctttaa
    15001 gcattatttt tcaagctcat caaagtaatt atatacgata acctcacctg tctctttatc
    15061 tgtagtcttt ataatgccct tagctccatt tacctcttct tttgtaaatt catcatctaa
    15121 cacaactttg aatttatctt cctttccata accttgttga aattcatctc tcaaaattgc
    15181 atttttttct gcttttggct tcaactcaaa ttcaattcca gatttcattt catcaaaata
    15241 atttaatact acaacctcat tattcatttt attagttgtc tttataatcc ctttagctcc
    15301 atttacttct tcttttgtaa attcatcatc caatgttata ttaaatttgt cttcttttcc
    15361 atattcctct tgaaaatcca tcttcatttc attgtattta gtttcaaact ctttactctt
    15421 gtttaattcc atagctgata catttgtcaa tgatgctcca attgccatta ctgttaatgc
    15481 cattactata ccacttttaa atttttttac tttcatatct tacctcctaa attaaatgtt
    15541 ctcaatgaaa ttatataaag aaaatcctaa gttttacagt ttcattcttg taactaaaat
    15601 tactattatg taaccaaact ttttgcttca actattcctt aaaagttgct tttttgagga
    15661 atagttgaag ttaaataatt agattttatc tatatttata gatttaaaat caaatttatc
    15721 catataaata aatacttatt tttgcaaaat aaaaattttt aattactcca atccttctta
    15781 tttacttaaa atcctatgat aagaaattta aaagtcttta ccttctttaa caataattta
    15841 tttactttta acatacaaaa tatagagagt atatcaaaat cattttactg aatattc
//
LOCUS       DACULQ010000090        15774 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.090, whole genome shotgun sequence.
ACCESSION   DACULQ010000090 DACULQ010000000
VERSION     DACULQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15774
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.090"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..496
                     /locus_tag="JA154_11140"
     CDS             <1..496
                     /locus_tag="JA154_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3793 domain-containing protein"
                     /protein_id="HAT7996416.1"
                     /translation="PAEIINISFTDVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLK
                     ILFVNKKALSEKLKCKKTVNFLKFLGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLG
                     YPLKDVIGFMGYSNYEVSMIKYWKVYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKI
                     VSCF"
     gene            complement(594..1586)
                     /locus_tag="JA154_11145"
     CDS             complement(594..1586)
                     /locus_tag="JA154_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7996417.1"
                     /translation="MIKRNKKILAVMTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPK
                     EINLTYVKSPLNVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFL
                     HALGGTSAIIAASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTI
                     LHQLLIAALAKEGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKM
                     IVNGEGLVSGIIVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVG
                     LSLDETKEMYSWYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(1587..2318)
                     /locus_tag="JA154_11150"
     CDS             complement(1587..2318)
                     /locus_tag="JA154_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996418.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(2311..3081)
                     /locus_tag="JA154_11155"
     CDS             complement(2311..3081)
                     /locus_tag="JA154_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996419.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(3166..3876)
                     /locus_tag="JA154_11160"
     CDS             complement(3166..3876)
                     /locus_tag="JA154_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HAT7996420.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(4056..4214)
                     /locus_tag="JA154_11165"
     CDS             complement(4056..4214)
                     /locus_tag="JA154_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAT7996421.1"
                     /translation="MNMATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSS
                     KSCHSAKR"
     gene            complement(4234..6390)
                     /locus_tag="JA154_11170"
     CDS             complement(4234..6390)
                     /locus_tag="JA154_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HAT7996422.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(6490..6711)
                     /locus_tag="JA154_11175"
     CDS             complement(6490..6711)
                     /locus_tag="JA154_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAT7996423.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(6724..6936)
                     /locus_tag="JA154_11180"
     CDS             complement(6724..6936)
                     /locus_tag="JA154_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAT7996424.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(7257..10154)
                     /locus_tag="JA154_11185"
     CDS             complement(7257..10154)
                     /locus_tag="JA154_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HAT7996425.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLNSGYEYSVLYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNIAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKYKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSNLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKANMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSIESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLKKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(10481..11086)
                     /locus_tag="JA154_11190"
     CDS             complement(10481..11086)
                     /locus_tag="JA154_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT7996426.1"
                     /translation="MDNLIKYIHKSYVAIVVLHEIYGINSFIEDVCQKYYKYGYDVFC
                     PELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGAT
                     IAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQN
                     IQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(11349..11894)
                     /locus_tag="JA154_11195"
     CDS             complement(11349..11894)
                     /locus_tag="JA154_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HAT7996427.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(12012..13835)
                     /locus_tag="JA154_11200"
     CDS             complement(12012..13835)
                     /locus_tag="JA154_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter ATP-binding protein"
                     /protein_id="HAT7996428.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(13848..15623)
                     /locus_tag="JA154_11205"
     CDS             complement(13848..15623)
                     /locus_tag="JA154_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996429.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         5241 a         2815 c         1541 g         6177 t
ORIGIN      
        1 acctgctgaa ataataaata tttcgtttac tgatgtaaat agagaggaaa aaattaatga
       61 tatttataaa tatctatata aatgtaagaa aatagatttt atagttattg ataaagaaaa
      121 aaagggatta aaaatcttat ttgtaaataa aaaagcatta tcagaaaaat taaaatgtaa
      181 aaaaacagta aactttttaa agtttttagg atacaaacaa aatacgaatg ttaatgctta
      241 tttagagcat ttagtagata aacttaagag tgatgttttt cctgatgaaa taggtatttt
      301 tttaggatat cctcttaaag atgtgattgg atttatggga tatagtaatt atgaagtttc
      361 tatgattaaa tattggaaag tttatggtga cacaaaacaa tccgaggaca catattcgaa
      421 gtttttactt caccgtaaga aaatgagaaa attactagac tacataagtg tagataaaat
      481 agtaagctgt ttttaattgt atttttgtat aataataaaa atacaccctc ttttaccata
      541 tagtttccat aataaataag ggtgtatttt ccactttaat attttaaagt attctaattt
      601 tgattgtaca acatatcttt aatatttatt ttcttttgtt gcattccatt agacattaaa
      661 aattcctgtg tatcttctaa ttcttttata tccttgtccg ttattttaga actaaagtca
      721 taccaagaat acatttcttt tgtttcatct aaagataaac caacttcttt agatacaaca
      781 tccatcactt catccttgtt ttcattcata tattttaaag tttcttcatg aaccttcata
      841 aatcttttta ctaattcagg atatttttca gcaaagtcat cacttacagc agtaactatt
      901 attccactta ctaatccttc tccatttaca atcattttgg cacctgaatt tatcgttttc
      961 aatgccactg gacctgcaag cattgctaca tcagcacttt tactttctaa tgcagaagca
     1021 gcactaggaa tatccatatt tacatattca acatcatcta tagtatagcc ttctttagca
     1081 agtgcagcaa tcaacagttg atgaagtata gtcccttttg gacctacaat ttttttacct
     1141 tttaagtctt ttatagattt aatattatca ttattagtta taagcatata tccttttggt
     1201 gaacggctat aaatattcgt aattttaaga ccaacaccat ttgatgcagc tattattgca
     1261 gacgttcctc ctaatgcatg taaaaagtct agctcacctg ctgctaatgc ttgagtttgc
     1321 tctggtcctg ttgtcaattc atggaaattg acttttatat tgtccttttt aaattccttt
     1381 ccaaataaat catccttttt ttctaatata ctaggaacat taagtggtga ttttacataa
     1441 gttaaattta tttccttagg taaactagcc tctttttttg caggttcatc tttcttatca
     1501 tttgttgttg aacatcctac agtcccaacc aacaccatta ttaaagatgt cattactgct
     1561 aaaatctttt tgtttctttt aatcaattaa aactccccca attcccttaa tatttctttt
     1621 ttcaagttta tataatagtt tttagttaaa tctctattat attcatcttc gacactaaat
     1681 tgtttaatcc ttttattttt actaaaaaca attattttct ttgctatttc catagcttct
     1741 tcaatattgt gagttacaaa tactacacct tttttagtat ttttatggat atttacaact
     1801 tctttttgca tttttcttct tgtgaagtaa tctagtgctg caaatggttc gtccatcagc
     1861 aaaatatcgg gattgaagga aagtgctctt gctatactta ctctgtgagc cattcctcct
     1921 gataattcat ttggatatga gtttttaaac ttttcaagct tcatcatttt taaatacttg
     1981 tctaaatcaa ctttatttct attatctttt tcagtatgta aaagtatatt ttcacttaca
     2041 ttcagccatg gcataagtct actttcttga aataccattc ctacttttgg agtacacttt
     2101 ttatcattat taaaaaaata tatgtttcca ctagtagcat tttcaagccc tgctaaaatt
     2161 ctaagtagcg ttgttttccc acatccactt tcacctaata taacagttat ctcttcacta
     2221 gatatattta aagatatatt atcaagaact aaatgctctt tattatcaac taaatatttc
     2281 ttatatatat tctctagcaa aaatccactt ctaaccattt aaatcaacct gcttcccttt
     2341 gctaaatttt gttattatct ttgaaaatag ataatctgaa agtataccta aaaatccaat
     2401 aacaaaaatt cctaccaata caacatcagt tctagacata tcttttccat cagaaatcaa
     2461 atatcctagt cctgaagaag ctgcaataag ctctgctcca attatagccc taaaactata
     2521 cccaagccct aaactaagtc caacaacaat atccagcata gaattaggga ttattatttt
     2581 ataaaaaatt ctctttttag ataaattaaa agatttacct acttctatta atttattgtc
     2641 acaatttttt atacctttca atgtatttaa aaatattgga aaaaaggatg ccaaaataat
     2701 aattataatt ttagattctt cacctatacc aaaccataaa ataagcattg gcaccaaagc
     2761 tagtggaggt gtacttcgca taaattcaaa tagtggtttg aaatattcat acattttttt
     2821 atttactcca aataaaactc caagtggtat agccatcaat gaacttatta aaaaacctat
     2881 taatatcctt cttacactgg cataaacatt taagaaaata gaaccgtcat atatcatttt
     2941 tataaaagta tctaagacta tttcaggagg aggcagaata tagctactcc atatacccaa
     3001 aacacatact atcttccata aaattaaaat tattaaaaaa atataatatc ctttaagcct
     3061 tttatgaatt ttctcaaaca tataaaactc cttttaacat atctatccct gctacccttt
     3121 ttatttttta atatcttaaa taataattaa gctatatcat aagatttaat ctatgccaag
     3181 tcttaatcca cctgaactca ttctatcaga aggtgtagaa tctgttttta agccagacaa
     3241 tctttcagat ggttgtataa tgactatttg tccaggttca acaaattcca tatgataaga
     3301 ttctcctgaa gtctgaccaa taaaagtctt ccatgatagt tttgatactt taggatatgg
     3361 ttctcttcca gtccaagcta ctaatgcatc tgggtcaaca caacaaggtc cttcaagaat
     3421 tagaggatta ccatctgtta ttatagcaac atcttgattt tgacctgttt tatttactaa
     3481 atgtgtagta aaaagacctt tttgagatat tacccctgaa ccaatcaatt ttacactgta
     3541 atttaattgt tcagaaaatg ccaataagtt ttcactctca acataaatgg aatcacctgg
     3601 gtcaattgat attacatcta catggtaagc attttgagct aggtaaattt caccttcacc
     3661 ttcaaccacc attattggca tttgttctcc tgtcaatgac ctctgaacat gacctaataa
     3721 tgctcttccc aatccaccgc cattacttgg acctaacagc aatttttcaa aattaaaatt
     3781 tcctttaaag gctaccatag cacctttttt tgcaaaaaat ttaccacttc cccttgctat
     3841 acacataagc tcgtttgtaa catcaaattt aaacataata ttcacttata ttgcttaatt
     3901 tagcaataat ccctcctaat catatattta tatataatta tacagtaaaa ttacagcata
     3961 gtctaaccta cttcaaatat gaaatttgtt atttatacaa aaaataagaa cctgataata
     4021 aagcaattta actttttatc aggctcttat cctatttatc tcttagcgct atgacaactc
     4081 ttactacttg agcatccaga acaaccacat ccacaactgc taccattctt ttttgaattt
     4141 ttaaccatat acattacagc tagtgccatt aatactacta caatagctgc tataataaat
     4201 gtagccatat tcatcactcc ttatttatat gaattaattt ttcatataac ttaatttacc
     4261 cattttttct tttttcatat ttttatttga ataagttaaa actacaaaga ctactgctac
     4321 tgctataaat atagatatta ttgccccagc aatacttcct ccagtaccta aaactaaact
     4381 tccaacttga tttataagaa gagcaattat atacgctgtt aaagtttgga atcctaatgt
     4441 aatccatgtc catttccatg aacccatttc tctttttatg gctccaatag ctgcaaaaca
     4501 aggtgcacaa agcatattaa atgccataaa tgcaaacgca ctagctactg taaacatgct
     4561 tgcaactgaa gcaagaagtg ttgggtcttg ctctgttgca tctgatatac caaataaaac
     4621 tccaaatgtt ccaactacat tttccttagc tactagacct gtaactgttg ctactgatga
     4681 ttgccaattt ccaaatccaa gaggagcaaa tattggtgca actatatttc ctaagctagc
     4741 taatatacta tctccagcat ctaccatttg taatgaccaa ttaaatgatt gtaagaacca
     4801 gattactcca caagcaacaa atataattgt tcctgctttg attataaatg cttttcctct
     4861 atcccacata tgtattaaaa caccttttgc acttggaata tgatattgag gtaattccat
     4921 aacaaatgga gatggttctc ctgcaaataa actagttttc tttaatataa ttccacaaat
     4981 gattatcatt gcaataccta agaaatacat tgatggagct acccatgaag cacctccaaa
     5041 taatgcccct gcaaataatg ctattattgg aatcttagct ccacatggaa tgaatgtagt
     5101 taacataata gtcatttttc tatctctatc attctctata gtacgagttg acataactcc
     5161 tggaactcca caacctgaac ttataagcat tggtataaat gattttcctg aaagaccaaa
     5221 cttacggaaa attctatcca ttataaaagc tacacgtgac atgtacccac aatcttctaa
     5281 tattgataat aataagaata aaagcatgat ttgtggtaca aatcctagta ctgctccaac
     5341 tccaccaatt agaccatcaa ctacaagtcc ttgtaaccac tctgctacat ttaaagaagc
     5401 tagaaaatta cttacattgc cttgaataat ttctccaaac agtacatcat ttgtccaatc
     5461 agttgctata gtacccaatg aacttactgc tatatagtaa actccccaca ttataagtgc
     5521 aaaaataggt aatgctagta tacgatttgt tacaatctta tctatcttat ctgatacagt
     5581 ttccttacct tttcgattct tctttattat acttgaaatt attgaactaa taaattcata
     5641 acgattggct gttattatac tttcactatc atcatccaat tcatcttcac aatttctagt
     5701 tatttcttca atactattca atattgtttt agaaatatca agtttttgta taacattttc
     5761 atctctctca aatagcttta tagctaacca gcgtgtttct acatctatat atggagtctt
     5821 ttcttctatt attttttcaa ttttttctat tgcatcctta ctttcatcaa caaagggtaa
     5881 tttgaaattt aatttattgt tactacttgc aagttcaact gccttttcag cagcctccat
     5941 aattccttga cctttaactg ctgttacttc taccactggg caacctataa cttcagatag
     6001 ttctttaata tttatcttat caccattctt atttacaata tccatcatat taagagctat
     6061 aacggtcggt atacctagct ctaaaacttg agttgtaaga tataaatttc tctctatatt
     6121 tgatgcatct acaatattta ttactgcatc cggcttatca tctagcataa aattacgtgt
     6181 aactacttct tctaatgtat atggagataa agaataaata cctggtaaat ctactatttc
     6241 cacatcttta tttcctttta attttccacc ttttttttca actgttactc ctggccagtt
     6301 accaacatat tgtgatgagc ctgtaagccc attgaacatt gttgtcttac cacagttagg
     6361 atttcctatg agacctattt ttattgacat ttccattccc tccattatta tttaaataca
     6421 tcactattca tgatatagaa aatcattctc attatataat tacattaact ctactctaat
     6481 taatctttat tattccacaa taattttttc tgcatctgac ttacgaattg atagttcata
     6541 atctctgact gttacctcaa ttgggtcacc aagaggagct acttttctta taaatatatt
     6601 aacgcctctt gtaatcccca tatccattat acgtcttcgt gtagctcctt ctccttctaa
     6661 ctttttgact tttacagtct ctccacactt aatatccttc agtctgttca tgtcattacc
     6721 tccctatatt attattctac ttgccatttt tttatctaat gcaactctag tatcttttac
     6781 gtctattatt aagttaccat ctatcttaga aactatttta acatttgtac ctactacaaa
     6841 acctatacta tttaaaaaag tcctcgtctt atcacttcct gaaattttct ttataactac
     6901 ttcttcacct gtatttgata gtatcaatgg catcatataa tatccctcct cttaaactaa
     6961 tttaaatcta caagcaatcg ttatcttata tataaattac aataccttaa atcttaatct
     7021 attaaaataa tatcctattt tttccttctt tttacaattc ctaattttga tttaagtcta
     7081 tcacaatagt atattttttg caagtgatta ttgtattctt ttgatttttt ttctcatttg
     7141 atttatttta ttaataaaat cgtaaaaatt aatacaaaaa taaagatgct ctaaaatagt
     7201 atactcaact aatattaaag cacctttatt atatatatca ttacttacac attgctttat
     7261 tcttttttaa aagcaatttt ttcaaactct tttacaggca ttggcttaga aaacaaatat
     7321 ccttgtacca tgtcacaccc aatttgcttt agaaattcta cttgcgaaat agtctctaca
     7381 ccttctgata ttactttcat tttcaattcc tttgccattt taatgacatt agatataaca
     7441 attttttcat tattactctc atctttttct gtaaaaaatg ctctatctag ttttaatatg
     7501 tcaacaggca attcttttaa aagattaaga gaggaatatc ccgtaccaaa atcatccata
     7561 gagattacaa atcccttttc tttcaaattg ttcattgtat caagtaaccc ttctatatta
     7621 tctaatactg ctgtttctgt tagctctaat tctataagtc ttgtcggaat tttgtattta
     7681 tctacaatta atttaaactt ctctataaaa ttactattta caaaatgcat tcgagacaag
     7741 tttacagata taggtactac tttttgtcct tcatccatcc attctctaat ctttttacac
     7801 acttcctcaa atacatagaa atcaatattt acaataaaac cattcctctc aaatacagga
     7861 ataaattcta ttggtggtat tagacctttt tgtggattat cccatcgaac aagagcctct
     7921 gcccctataa tttgataatc tgaaagacta tatttaggtt gaaaataaac aataaattct
     7981 ccattattaa gagcatctac cattgaattt tcaatttctt tctcttttaa tattttttta
     8041 tgcatttcct tatcgtaaaa agcaaatgaa tttttatgac caccttttat agtttttctt
     8101 gcagtattag ctctatccat aatagtattt atgtcattat cacttttact tattttatat
     8161 ataccacaat ctaaaatcaa tttataatag tttattttag gattactaag tatttgaacc
     8221 ttattgtaaa tattgtttaa ccttgtttta atatcatcat ctgtcttata cttaataaga
     8281 agaacaaaat ggtcagcaga aactcttgca aaaatttcat cttcctttaa ttcacttgca
     8341 atagtatttg atatatgtat caaagtatca tttcccatat catatccaaa catatcattt
     8401 atatatttaa atttgtcaac atctatataa aacaatgcat attcttctgg attattttta
     8461 gcaaacagtt tattcgaatt tattttaaat ttatctatac tatctgctcc tgtcaatggg
     8521 tctgtatatg ctatcttctt tagataatcc tctttcttct ttttattata aattatatat
     8581 ttaatagcaa ttaagaataa aatacataca aaagaaataa tttgtattga atattcttta
     8641 aaaatcacac caaatggtac tttataaggt ttgtcaatag tattagaata tatacaatca
     8701 cttaattctt cttcagtaat tttgtctatt gatttattta aaatagatac taatataggg
     8761 tcagttttat ttgatacccc tatacgcatc ccatagcttg tatctaaaac agatataaca
     8821 gataaattct cactctttcc ttctcttaaa agttcatcaa actgataaga actaagtatc
     8881 attaagtttg ctttaccttc atttatagcg tttagacaac tttctatact cttataagat
     8941 gttgtcttag cattttcaaa acttttttct atataagaat cgatagactt ataactactc
     9001 gttaaagcaa tatttaaatc attattgaga ttggtatcca gttttcctac cataatcatt
     9061 ggaatattta tgtatggatt tgtcaatttc atattaaatc gttttgcctt atcattttca
     9121 ttgattgagc cacatagcat atttgcctta ccacttttta tgtaatcaag actctcatta
     9181 taattttttg ttttaatata ttcaaatttt ataccacatt tttttgatat aattgacatt
     9241 aaattggaac ttataccttt aaacgaattg ctttccttat cataatactc tacaggagac
     9301 cactctgaat catataccat agtaagtttt gggtttgctt taataaaatt catttcttgc
     9361 cgagtaaatg ctatggaatt attgacctct cttttgaaat attttttata taattccaac
     9421 tcataataca tatcatttat ttttaattct tttaaagcat aatctaattc ttttaccaaa
     9481 ttaggtttat attttgcagt agcaaaataa aaaggctcta ctgaaaattt gctaactatt
     9541 ttcattcctt tagcatttat aatacttcca cagacaaatg catctgcctt tttttcattg
     9601 acagactcaa ttaattcatc aatagaatta cagtagatat attcaactga aaaattattt
     9661 tcttctctat acttctcaaa tgcaatatta tgaaagtttc ctctaagtac agctactctc
     9721 attcccttaa aggaacttat atcttcataa aacatattat tgttactatc ttttgtatat
     9781 agtacactat attcatatcc tgaatttagt ttaggaaaat taaatagttt atttctttca
     9841 tcattcttct gtaaaggtcc aagtaagtct atttcacctt tttctagtct gtctaaacat
     9901 tcttcccaag acccttttac aaattcatat tcccatcttg tatattttga gatttctctt
     9961 agataatcat aaccatatcc tgtataagaa tgctcttttg atgaggatat tattccgtag
    10021 tcatcacaat atccaactct tatttttttt gaattttcag actcatcagc aaagcatagt
    10081 atattgttaa aacatgatat aataagtaaa aatatcaaag aaagtatcaa gaagtgcttt
    10141 ttaaatgctt tcatatttaa aaacctccaa tttgccaact tactttaact tatttggttt
    10201 tctagttgta actactttta ttgtaatggt aaatatatca aattgcaata ttctcatata
    10261 atattcttat tcaactataa tgaatttatt attatctatt taaatacact atctaatatt
    10321 agtttcttat aagtcataaa agcacaacta acactataat tctagtgatt aattttattt
    10381 caaaaaatat aataacacta ctaatatttt aaatatcaat agtgtcataa aaacttttca
    10441 gattaattaa atctttctat atattttata ataatctaaa tcatgctaaa tatttagaca
    10501 gaaaactttt ttctttaata gtaaatactt ttgtttgaat atcagaataa ttatttgaat
    10561 atctatctat aaaaccatga tttgcatcta aaatctcaat ttggatattt tgtttcctag
    10621 aaagttggta agaaatagaa tcaacatcaa atgaatcgtg ctttgcaaat actaataagg
    10681 taggacattt tggaattaca tccatgtaat ctcgaatccg agagccataa caacaaatta
    10741 tgccatcaca aaaagaatct tcactgcatc tccatgcaat agttgctccc acactatacc
    10801 cagcaacaaa tacatattta tactctgatt ttaaatcatt tataagcttt tcaacttttt
    10861 tataaatatc aaaaccaaca ttatttataa aaaaatcata tgcctcttgg acttttgaat
    10921 atggaaaaac tcttttttct cccagaagtt caggacaaaa tacatcatat ccatatttat
    10981 aatacttttg acaaacatct tctataaagc tattaatccc atagatttca tgtaaaacaa
    11041 caatagctac ataactttta tgtatatact ttatcagatt atccaccacc tttacaaaac
    11101 agcaaatttt ttatacttag tttaatatat tcttctttac atgtcaattt acttttctaa
    11161 aatcaaattt atttttggat taatcattta tttttaaaag ttgtgcatta ttactattaa
    11221 attattctct attattaaaa atataatcgt tagaaataaa aaataaagta caaataatta
    11281 aaaaatatct cactcataac tttaaaaatt gtaagtgaga tactcctaaa atctacttta
    11341 tattaaattt attttccaaa atatctattt aataatccta agaatgcttt accatgtcta
    11401 gcttcatctt tacacatttc atgtactgta tcatgtatag catctaatcc taattctttt
    11461 gctttcttag ctaattttaa ttttccatca gttgcaccat actcagcatc aactctaact
    11521 cttaagtttt cttttgtatc tgcaactaca acttctccaa gaagttctgc aaatttagca
    11581 gcatgttcag cttcttcaag agctattctc ttataagctt ctcctacttc tggataacct
    11641 tctctatcag cttgtctact cattgctaaa tacattccta cttctgtaca ctcaccaaca
    11701 aagttagctc ttaatccttc gattatttct tcatctacac cttgagctac tcctattctg
    11761 tgctcatcag cccaattttt ttcgcctttc atttcttcaa atttatcagc tccaacttta
    11821 catactggac attgtgctgg tgcagcatct ccttcatgta tatatccaca tactgtacaa
    11881 acaaatttct tcataattac tttcctccta aattttatat atcaaattaa attttttatt
    11941 tattaagtta gtctatatat acccgcacca tataccttta aacaattttt ttaaaagttt
    12001 tttctaaata cttattttcc ctgtgtcaat aatatgtttc tataataatg tccctttaat
    12061 tttatcaatt ctttatgatt tcctgattcc acaattctac cattttctaa aactattata
    12121 ttatcacaat ttgatatggt acttaatcta tgagctataa ttatacttgt ctttccataa
    12181 gttaaattat ttattgcttc ttgaatttta gcttctgttg ctatatctat gttacttgta
    12241 gcctcatcca aaataagtat tggagcattt tttaatactg ctctagcaat acttattaat
    12301 tgtttttgac cttcacttaa atcttcaccc ctatccgata atacagtact atatccttca
    12361 cgcattttaa ctataaattt atgtgcattt gctattttac tagcatatat aacttcttca
    12421 tcagtagcct ccagatttcc ataccttata ttttccataa ctgtatcatt gaatatataa
    12481 ccttcttgtg gaactactcc aaaacaagtt ctcaaatagt ttctgttaat agagtatata
    12541 tccttaccat ctaaaaatat acttccttca tctatatgat aaaaatttgt aattagattt
    12601 ataatagttg tttttccact tcccgtctct cctatgatgg catttgatgt gccagatttg
    12661 atttttaggt ttatattatc taaaattttc ttttttgaat cataagaaaa acttacatct
    12721 ttaagctcaa tttctccctt tacaaaatga gagtttaaat taccatcttt attatcgtta
    12781 atctcacttt taaacatgtt atgcacttct tgctcttctt gttcatctat aatttcaaaa
    12841 actctttcac atccagctat ggctgaaagt aatgtattaa atagatttgc taattcattt
    12901 aaaggtcttg tgaattgtct tacataactt aaaaaactag ctataacccc tatggttata
    12961 atatctttag atgcaagaat gcctcctata atacatatag ttgcaaatcc tatattacta
    13021 atcacattca taataggcat taaaagagaa gtgtaaattt gcgatttaag ggaaatattt
    13081 agaagtctat cattgacttc tttaaaatca tttacagatt ttctttcata attaaaagat
    13141 tttactacat ttatgtttga tatcatttct tcaatatgtg catttagttt tcccaactca
    13201 acttgttgtt gcttaaaaaa aactctagtt ctctttgcaa taaaattact cacaaacatt
    13261 actatcggta ttaatattac actgcaaaag gttaaaattg ggcttaatac aaacatcata
    13321 ataaaagtca tcaatatgtt taagattcct gtaattaact gtactaatga atttgatatt
    13381 cctgtactga tattatcgac atcatttgtc actctactca taatatctcc agtagaactt
    13441 ttatcaaaaa aggttattgg tagattttta aattttaaaa ataaattact tctcatttct
    13501 ctgactatac tttgagataa tctagctgta catattccct tagacagttt aactgaaaac
    13561 tcaattatat aaaatgaaag taaaataatt attcctttta agaaaatgga ttttgaataa
    13621 gtatcgatat ctattatgtc aactaccttt ccaatagtat atggtattac agttgatata
    13681 attgaatcaa ttattactag caaaaaaata aatctagtaa tcttcttatc ttttttaaag
    13741 tacataaaaa ttcttttaaa cgttttttta aaatctttag gcttttcttt tttctttaat
    13801 ctactgacct tctgatttcc agtacttaaa ttcaatatac ttcctcctta atttacatct
    13861 tcacttacta aattttgcaa cgcaaaaatt tttttataaa ttggagaact aaccagtaaa
    13921 tcttcatgtg tagcaaatcc aaccacctca ccctcatcca agactaaaat cttatcacag
    13981 tcaattatag aactaatctt ttgtgaaata gttattacag tacatttctt actttccttt
    14041 tctcttaaat tacttatata ctcttttata ttctttctta taattgtctc tgtattaata
    14101 tccattgcac taaaacaatc atcaagaatt aatatttgtg gttttttggc tatagctcta
    14161 gctatagtaa ttctttgtct ctgtcctcct gataaatttt tacccctttg gaataaaact
    14221 tcatcataag tattttcgag ttttgttata aattgatttg catttgctat ttcacatgat
    14281 tttacaacat cctctttgca tatatctcta tttcccataa ctacattctc caatatactt
    14341 tttccaaaca cactactatt ttgaaatact acacctattc ttgcccttaa actttcttca
    14401 gaaatactgt atatactacg tcctccaatc ttaatatcac ctttatcaat tatatataat
    14461 ttttgaatta aatttactaa agaagtcttt cctgaaccag tttgaccaat cacaccaata
    14521 ttttgacctt cttctatttt aaaggatacg ttttttaata ttttttcatc tgtatttttg
    14581 ttatacgaaa aatctacatt ttcaaaaatt atatcacagt ttacaaactt taaatctttt
    14641 tctccaacat ctaaactttc ttctctctca attacttctt cattctcatc aaccaaaacc
    14701 tcacttattc tctcataaga tgcttttgcc ctaacaaaaa cattaaaaac atgtgaaatc
    14761 attataagag atgttaatat tctagtcata tagtttacat aagcaactat aatcccaatt
    14821 ttaatttctc ctgaataaat taaatgcctt ccaatcagta atattattat tatggaaaca
    14881 ttaatgatta ttgtaattgt cggtctaaaa aatgacatca tagttgtagc ttttctacta
    14941 tttttaaaaa gttcatcatt taactcttca aagctttggt tctcataatc gtatctacaa
    15001 aatagtttaa ctacttttat tccacttaaa aactgtctta attttgaatt tactttatct
    15061 gtactttctt gtacattttt aaataatcta taccctatct tcatattaag caatattata
    15121 ataaatataa ttaaaactgc aattagaata attattgaca tctttaaatt taattttata
    15181 gctataatta aacttcctat acacattatt ggtgacttta taaaaactct catcattcca
    15241 ttaacgaatt gttgcatttg attgatatca ttagtaattc ttgttattaa tgaagctcta
    15301 ccaaatttat ctatttgttt taatgagtat gtatttatct tttcaaatac atcctgtctt
    15361 aaatcataag aaaaatgctg agaaactctt gccgagatta cacttcttat tattgcaaat
    15421 attgcaccta aaaaagttat acctatcatt atagctcctg ttgatagtat gtagtctaag
    15481 ttcttatttg aaacccctat atctattatt tttgccatta tagttggttg agctaaatca
    15541 caaaaagctt ccaatgttaa aaaaaatact gctgttagaa ataatactat atattttttt
    15601 atgtattttt taaaatacct cattcaatca tcccttcata gaattttctc attaccaaaa
    15661 gtatatcata atggctaatt taattgatta tatcaattat aaatatttga tttattatta
    15721 atttttatac catatatttt ataaattatt tgtttttatt tcctatattt atat
//
LOCUS       DACULQ010000091        15746 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.091, whole genome shotgun sequence.
ACCESSION   DACULQ010000091 DACULQ010000000
VERSION     DACULQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.091"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            67..1470
                     /locus_tag="JA154_11210"
     CDS             67..1470
                     /locus_tag="JA154_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996430.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            1689..4166
                     /locus_tag="JA154_11215"
     CDS             1689..4166
                     /locus_tag="JA154_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996431.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSKELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4181..4867
                     /locus_tag="JA154_11220"
     CDS             4181..4867
                     /locus_tag="JA154_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996432.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            5141..5974
                     /locus_tag="JA154_11225"
     CDS             5141..5974
                     /locus_tag="JA154_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7996433.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            6012..6473
                     /locus_tag="JA154_11230"
     CDS             6012..6473
                     /locus_tag="JA154_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HAT7996434.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            6504..6752
                     /locus_tag="JA154_11235"
     CDS             6504..6752
                     /locus_tag="JA154_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HAT7996435.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            7205..7525
                     /locus_tag="JA154_11240"
     CDS             7205..7525
                     /locus_tag="JA154_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HAT7996436.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            7663..8307
                     /locus_tag="JA154_11245"
     CDS             7663..8307
                     /locus_tag="JA154_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAT7996437.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            8436..9182
                     /locus_tag="JA154_11250"
     CDS             8436..9182
                     /locus_tag="JA154_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996438.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEI
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(9311..9589)
                     /locus_tag="JA154_11255"
     CDS             complement(9311..9589)
                     /locus_tag="JA154_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996439.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            9855..10310
                     /locus_tag="JA154_11260"
     CDS             9855..10310
                     /locus_tag="JA154_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996440.1"
                     /translation="MRYMESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKY
                     FESIGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVV
                     FGFIGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            10580..11032
                     /locus_tag="JA154_11265"
     CDS             10580..11032
                     /locus_tag="JA154_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, biotin carboxyl carrier
                     protein"
                     /protein_id="HAT7996441.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            11048..12409
                     /locus_tag="JA154_11270"
     CDS             11048..12409
                     /locus_tag="JA154_11270"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088444.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAT7996442.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            12424..13278
                     /locus_tag="JA154_11275"
     CDS             12424..13278
                     /locus_tag="JA154_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HAT7996443.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKLLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            13299..14243
                     /locus_tag="JA154_11280"
     CDS             13299..14243
                     /locus_tag="JA154_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAT7996444.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            14432..14692
                     /locus_tag="JA154_11285"
     CDS             14432..14692
                     /locus_tag="JA154_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HAT7996445.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            14845..15216
                     /locus_tag="JA154_11290"
     CDS             14845..15216
                     /locus_tag="JA154_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HAT7996446.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            15246..>15746
                     /locus_tag="JA154_11295"
     CDS             15246..>15746
                     /locus_tag="JA154_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996447.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILG"
BASE COUNT         6116 a         1664 c         2576 g         5390 t
ORIGIN      
        1 caataattat tgtctatttt atataagcaa taatcaaaat aaaagtttaa gaaaagagtt
       61 gattaaatgc ataaacctag ttataaaaca gtatttcatt catacattat ttttttcatg
      121 atttttattt gcttaatcct gacagtaata ggtattttct tatcacttat tactattcag
      181 aaaccaaatg gggaatttat tcgtagtgac tggccgaaaa aatttacaga aaactttaat
      241 gaacaaatcg tatttataga taataagcca caagtaaaac agtctggatt agaaaaactt
      301 cgagaaaatc aattgtggtt gcaaatttta gattctagtg gaaatagagt ttatgggttt
      361 ttagaaccaa aagagcataa agattattat tctaattctg aattattagc attagctaac
      421 caaaataatt caagtaatgg aactgtatac tttaatacaa ttaaaaataa taaaaatgac
      481 tatatttata ttatatattt tccattaaat atatctaaag tgaatatgtt ttttgatgga
      541 ggtaaattta taggtggtaa gtcaattgta ttttcaatca tagggattat atttttaata
      601 attttggttt ctggaattgc ttatggttac tgggtaacaa agtgcatatc aaatattaca
      661 tctgctgtta gcgatatttc aaaacgaagc tatcttccag ttaatattaa aggtgttttt
      721 gctgatatat ataatagttt aaatatttta aatgaagaaa ttcatagaag tgatgagata
      781 caaaataaaa ctgatattat gagagaagag tggatttcaa atataactca tgatttaaaa
      841 actccacttt caccaataaa agggtatgca gagcttctta cagatagtag tactaattat
      901 tcacctgaaa aagtatataa atatgcttca atcatgttaa agaatataga atatacaaat
      961 acacttataa acgatttaaa attaacctat caattaaaaa atggaataat ccctttaaat
     1021 aaaaataatt atgatttagt aagatttttg aaagaattat ccattgatat tttaaactat
     1081 ccagaatatg aatcaaatat agttgaattt aagagtaata tagataatat taactttgat
     1141 tttgattgta cactattaaa acgagctttt agtaatctta ttgtcaatgc ttttacatat
     1201 ggagataaaa atactaaagt ttgtgtacat ataacattaa atgaaagaat tgaaatcatt
     1261 atatctgata atggaaaagg gatgtcaaga gaagaagcat caaatttgtt taatcgttat
     1321 tatagaggaa gcaatacaaa acaaaaaata gaaggaactg gtttaggact agcaataacg
     1381 aagcaaatta ttgaagttca tggagggaat atatctgttg aaagtatacc tggattaggt
     1441 agctcatttt atatcacctt tcctatttaa ttaaggttaa ttaaggtcaa ataaaaaata
     1501 tattaaggtt gatagattat tatatttata tagtagttta tcaatctttt ttatttaagg
     1561 ggtgatttga ttaaaaatat tataaataga atgataaaag tatggaggag tctattagag
     1621 atttaattta gattaaagta taaaaatatt agattgaaat atcttagata aggaaagaag
     1681 gtgtaaattt gaaaattata ttaaagtata ttttcacaaa tattaaagaa cgaaaaatta
     1741 gaacagcagt tatgttattg tccatagttt tatcaacagt tttacttttt gtttcattct
     1801 caattggatt atcttatgaa agtgcacaaa gaaaaatggc aaaaggtatg tatggtacag
     1861 cgactatatc tgtccaaagt aaaaatccag atatattaac caatttagaa gacatacctg
     1921 atttaaatgc tataaaatca aaggttggtg tattggaaag ctctgctatt tataataaag
     1981 ggggatacta tgaagaattt agtcttatat cagcagactt aagtcaacta aataaaatca
     2041 acaagccacg attagaaaat ggcgatagta ttactgattt ttctggtgat aaaattattc
     2101 ttccaaatcg ctttacatca aaatataaaa taaaaaaagg tgattctatt actttgcaga
     2161 tttatggaaa gtcttatact tttcaagtta gtgatattgc atcttatgat acagtatttt
     2221 tgaggaacac aagaggtgta aatgcactgc tacccaaaga gactctatca aaaattataa
     2281 ataaaggcag tggatataca agagttctta tagagtcaga agaagaaatt acagaaaatc
     2341 tagtaaataa attatccaaa gaactttcta ctgaaaaata tatggtatca aatactataa
     2401 atgaaactaa gataatttca gatgctagac aaaagacaat gcctttcttt ttaataagtt
     2461 tttttgcatt aacccttagt atatttataa tatacagcag ttataaggtt attacattag
     2521 aacgtcttcc ttttattgga acatttagaa gtattggtgc caatgaaaaa actgtaactc
     2581 gtatattgat gcttgaaagt atattatacg gaagtatagg aggattaatt gctattccta
     2641 ttggtgtagt tgttttaaat ttaatgcttc atgggttagg aaattctctc gaacaaggaa
     2701 tatcaattcc agtagtaata tcacccattg gagtgattat atctgttata gtagcaataa
     2761 tcgtttcttc atttagtgca tatatacctg ttaaaaaagc tagccatttg ccgataaaaa
     2821 atattgtact aggaacagta gaagaaaaga atgtttcaaa tcgttctata ctttttatag
     2881 gaagtataat gtttatttta tctattttac ttccaagaat atcccctgaa aatacattgt
     2941 atctagctgg tggattttca ttactgggat taattgtagc tactattgtg ttgattcctc
     3001 ttattactga tatcatgtct atagtttttg agtttgttta taaaaatatt ttagggaatg
     3061 agggtaaatt agctgctaga aatatgaaaa ataacaaaaa tataatacag aatatcactt
     3121 tactatttat aagtatttct gcagttattg caataagtgt tgttggtaac tttgtcaaaa
     3181 cttatataac agatgtgttt cgtgacgcag aattacaagg ttttgcagat ggaaagatga
     3241 atgaagagtt tattgaagat gttagacata tggatggaat taaaaaaata ttacctcttt
     3301 atgttatgaa taatgagatt tctggtaatg gtgtaacatt atcaagacta gagggtacag
     3361 ataatataaa actatataac tctatgtttg gaatcaatta tacaaatttt gagataaaaa
     3421 aacaagtaat agaagctttt aatgataaac gttctgttat tttgaatgaa gatactctaa
     3481 aaaaagtagg cttatctata ggagatacaa taactctatc taatgataag tatgattttt
     3541 cttataaaat agtaggtagt tttaaatccc gcgcaaatga tgtagaagct gttataccat
     3601 cacattatgc agttaatgat tttgataaaa ctaattatgg ttttttagta tatacagcag
     3661 tcaatccaga tgctgttatg attcagataa gatatttatt tggagataca tataattgga
     3721 gtcgtacagt tgaagaattt aataatgatt ctttaaatac tataagtagt tttttgtctc
     3781 ctatgaataa gatgacctat ttcatttttc ttcttgctac agtaggtatt attaataatc
     3841 tactgattaa ttatattcaa aaaagacgtt ctatagctat gtataaatct ataggactaa
     3901 gtaataaaca aaatataaaa gtgacattga ttgaagggtt tacttcaggt ttacttggag
     3961 ctgttattgg tattgtgata tctatacttg agattcaaac tatctttata gttgcaggac
     4021 caaaaatttc aatgaaacca gatttagatt ttaaaacatt tattattgtt gggttattgg
     4081 gaattatagt aactttaata ggttcgatag tgcctattat aaaagggaag aaaatgaaac
     4141 tgatagaaga aattaagttt gaataattat ggaggttaat atgaaaatta cacataatag
     4201 tttagctgta gaagcaaaaa atattatcaa agaatataaa attggaaata caacaactcg
     4261 agtgttaaaa gaggtatctt tacaagtaat gaaaggtgaa tttgtttcta tcatgggtca
     4321 atctggttct ggcaaaagta ctcttttata tattttaggt ggtttagata ctccaacgag
     4381 cggaaaagtg tatatgaatg gagctgatat atcacatttt aatgatgaga aaatgagcat
     4441 aatacgtcgt agaaatatag gattcgtatt tcaattttat aacttaatac caaatttaaa
     4501 tgtggaggaa aatataatgc ttcctttgtt attagatggt aaaaatttaa aagattataa
     4561 aaatcaatta gatgaaatct tagatatagt tggtttaact gatagacgta aacatacacc
     4621 acgagagtta tctggtggtc agcaacaacg tgttgctata gctcgagctt taattggaaa
     4681 accagagatt ctatttgctg atgagcctac tggaaattta gatagtaaaa cagggataga
     4741 aataatagat ttactgaata aaataaacag agacaatgga caaactataa ttatggtaac
     4801 tcactcacca gaagcagcca aaagtagtag tcgtactatt acagtttcag atggtttaat
     4861 tgtataagga ctaattattg tatttgagat aagcattttt aattatataa attagacata
     4921 tgtaaagtgg atataagtta aatactataa atgtgctata atattaaaat aacagataaa
     4981 agtactatat tttttattga ttattttaat tttatttgtt tattaaatat ctaatgagta
     5041 ttcatactat ctctattatt aaaagcagtt aatgatatat agagataata tggattcttc
     5101 ttactttaat aaatattcac gataggacag gatataatat atgttaaaaa aagaaagtat
     5161 tcattttata aataaaaata ggtcacttat agtttgtgaa gacaattttg tttatctctt
     5221 gccttgtata caattaagaa attggatatc gaattttact atatcgtttc caaatcaaac
     5281 aataatttct gataattaca cgattattcc tcatggaagt gtaactttgg tgttttttta
     5341 tgatgccaca gggttacata gtctattatt tggtccaact actaaaccaa aaacagtagg
     5401 aaatattgct aatagatgtg atgttatact aattatagaa tttcaacctg ctggtttttt
     5461 cccgcttata ggtatacagc aaagtgaact tattgataaa gttgttccat tttctataat
     5521 taatacttca ttagatttag aaattaaaaa aatatttaat gaatctttat ctatagataa
     5581 attgatttta aagttggaag aattattgat ttctaatatt aaaattgaat actcatatga
     5641 gtttattttg gcaattcagt tgattattca aaactctgga aatatatctt cacaagaaat
     5701 ttctaaaaaa gtattttata gttctaggca tttaaacaga ctctttaatc agtacctagg
     5761 gttgagtatg aagtcatttt ctcgtttggt acgtattaat aaatctataa aacttctaaa
     5821 caataataaa acttcattga tgtctatatg taatgaatta ggattctatg atataccaca
     5881 ttttattaaa gattttaaaa ttgtctgtgg aataactcct caaaaatacc gagctaatat
     5941 gtctgatttt tacagtgaaa ttgcaaaatt ttaagttata atacttataa caaagaaagg
     6001 atagtgataa gatgaaattt ggaggagtat tgctaattgt caaagacata aataaatcaa
     6061 aaaagtttta tgaagatgtt atggagcaaa aaatcacatt agatttagga gaacatgtat
     6121 catttgaaaa tggatttgga ttacaaagta attataaagc tataattgga tatgatttac
     6181 caatatatca acaatcaaat aactttcagt tgtattttga ggttaatgac atcaaacaat
     6241 gggaaagtaa gataaataga ataggaaata ttgaattttt gcattatata aaagaatacc
     6301 catggggtca aagaactttt aggttttatg attttgataa aaatattata gaaatttcag
     6361 aaagtatgga aagcgtcata aagaggcttt taaaacaagg tttagcttta gaagaaattt
     6421 caaaacgcac tatgtatcca gttgaattta ttacacaatt tcaacaatta taaaatatta
     6481 aaatatataa ttttttcttt atattgcaaa aaacaaaggc ttggggcata atagaaccag
     6541 aaaaacctga atctcaacag caaatggctc atatggattt cgctgttaat gatttagaag
     6601 aagcagttca atatgcaatt cattgtggag ctacaatcgc agaggagcaa tttactgatg
     6661 actggagagt tatgattgac ccagctggac accctttttg cttatgtcag atgaaatcaa
     6721 ttatggaaag tagccatttt tcattgttat agaccaaaat attgtttatt aattacaaaa
     6781 aagcacaaca gtatataccg ttgtgctttt acttattata catgaataca ctaaaaaata
     6841 taaatagtag agctttatca ataatgataa tttttaaaaa atagtatttt tttattgaat
     6901 aatttcttag atattttaaa gtatataaat tttgcatata tgaattggac attatcaaat
     6961 gtgttataat attaaaacaa caaataaaat tattatattt tttagaataa taattttatt
     7021 tgtttactaa gtttatgata gggtatttat ttaaggtaat aaaatattat ataaaatatt
     7081 ttaaagcctt aggtaatatt aggtagaagt tttagtaaaa tatgcaaaaa ctaattgatt
     7141 atgtaccttg tatgggtata taatagatat tgttttaata aaatatttga aagggaatga
     7201 tttgatgaaa attactttac cagaaacagc tattgatact ttaaaaagta tcttaaaaga
     7261 caatcaagat aaacctaata atataagagt atactttgca ggtgttggtt gtggaggtcc
     7321 ttcttttgga ttagctttag atgagaaaaa agaagatgac ttaacttatg aagttggaga
     7381 attacaattt gtaatgagct ctgatgaata tagtcagtat ggagatataa taatagaaga
     7441 tacaggattt ggatttagag ttattcctga aaatatgaaa gatcaaggtg gaggaggatg
     7501 ctctggttgt tcagggtgcc actaagatta agtttttagt aagtttagaa ctctctttat
     7561 agaaataaat ttctatttta agagagttct ttttattttt tggtgagata atgtataata
     7621 aaaatgtatt ttactaattt tttatatgta aggagaatta ttgtgagaag aagacgaaaa
     7681 aaaggtgcag atgaaaagct tttaagttat acaaaatatg ttttaagaga cgatatagac
     7741 aaattaaaag gcaagtggaa tttaaagttt agaaatgata atccaataca tgttgaattt
     7801 gggactggaa aaggtaagtt tataacaact ttagctaagc agaaccctga cataaattat
     7861 attgccatgg aacttaagga agaagttctt ttaaaggctg ttgaaaaagc agatgcttct
     7921 aatttgaata atatactatt tttatgggga gatgtaagca atattttgga ttactttgaa
     7981 gctaaagaat tatcgagaat ttatataaat ttctgtgacc catggcctaa aaatagatgg
     8041 agtaaaagaa gactcactca ttctggattt ttagaaatgt acaatagggt tttggaagat
     8101 gatggcgaaa ttcactttaa aacagataat gaaaaattat tcgagtttag tttaaatgaa
     8161 attgctgcaa ataattggct attaaagaat atttctcttg atttaggaaa tagtgagtat
     8221 gaaaataatg ttacaacaga gtatgaagac aaatttatgt cacaaggtat gcgaatattt
     8281 agatgtgaag ctaaaaaacg taactagtaa tatgtttata agtttaatag tagaaattta
     8341 ttttaattta tattaataaa gtgtacgaaa gtaggtatat ataacaagga aacagaaccc
     8401 tctcatagaa attaaattat tataataaaa aatttatgtg ggagggtact atgaatttag
     8461 ctaatttaat accatttatt atttctgcag cagctttaag tccaataaat tcaaatgata
     8521 aggttttgag cgaaaatcaa cttaaagata tttttcctga tgcaaattta agagcagttg
     8581 taaaaagata tataaatcca gatgagatga ctataagcaa tataaaggct ttagatggag
     8641 aattttatgc aactggtgag agcatttcaa atttaaaagg tatatcatat cttgaaaatg
     8701 tagacaattt tattttttgg aataataata taaaagagat tcctaaggaa gctttaagtc
     8761 taaaagatat ggattcaata aatcttgcca ataattattt gattgatgac gatgtagtaa
     8821 attctctttc tcataatggt gtggatgtaa attgcgattt aaattttatt gatacaaaag
     8881 ataatcagta taaactggac tctaaatatc ataacgttga tatagcaaaa ggtgaaagta
     8941 ttgatttgag aaaaataatt acgaagaaaa ttgatagtta ttataaatac tgggaagtta
     9001 ctgataacct tccaaaagat ttaaatttta ttgtatctgt aagtgataag agtgtacttt
     9061 catgtgaaga tatgataatt aaaggtaata aaacaggtca agcagtggta aaagttatac
     9121 taagcgacaa aaatgattta aatacatctc aagaagttct aattactgtc aatgtaaaat
     9181 aatctgtcaa cttattcaat cattctattt tcaatttaag tgtataatca aaataaaggt
     9241 tgttagtaac agaacgctaa tattagtgct ttgttactag caacctttaa attttataga
     9301 aatagattat ctaaaaaatt ccatttcttc tgttagtagc ccatattcct attaaaatta
     9361 tcagtgaata tattatatac aaaactattc ttatatattg gacatctaat ccaaaaaatt
     9421 caatgttact ctgtgaggta tgcaacatca caaggactgc agatatacca ataagcaaag
     9481 gattgattac tatttgctta tcagttctta aaatcattat tttacccatc caaattattg
     9541 ataatataaa aaataaaatt ttaagaccaa attctaatgt atctaccaaa gcgaaaacat
     9601 cccttcattt ataataaatt tttcagctca acaaaatata aaaccctaca aacaactttt
     9661 gaatccataa taagataact tgattaagca ctatgaaata agtactataa aacttcatct
     9721 acattaatat atactacact atacttcaaa tttcttaaac ataaattaat gaaattaaca
     9781 aaattttatt cagaaacata taaagatata ttttactatt taaatgcata tcattatata
     9841 tacagtttta ggaggtgagg tatatggaat ctaaattttt taattggtac actcaatcct
     9901 tagggggaac ttttggcatt atatcatgta tatgcgcata tttaaatgga tacatgtttg
     9961 tttatacaaa tagtgctagc aaatattttg aaagtatagg atttctggga ataatttcaa
    10021 gttatacact acttccactt tgtatgctta ctttgatttt tggagttata tattcttata
    10081 ctccagaaac atccgacaaa agtattttta atataccttt ttttacttta aataaaagat
    10141 taatacttat tacagtagtt tttggtttta taggtgctag agtttacttt ataattcctg
    10201 caattctaat aacttttaac ctatatagca atgttttgat agctaaatta aagtacatcc
    10261 tatgtaaaaa taaagaaaat gatactaatt tgaaagaaag cgaagtctaa attgcataat
    10321 attggtattt gtcttaaaaa ttcccgaggt attaatttgt attctcttgt attttgcaaa
    10381 aaatattgaa ttttattcat tttatactta taatttatat taataatgga aaattaaaac
    10441 aaatataagt tactggttgt taatttaaaa ttaaaagata tattgatgtg ttgattctct
    10501 agtcagcact ttttctttta taaatttgtt ttttgtgata taatattagc accaggtaat
    10561 aatacaaggg ggtaacaaaa tgaacatcaa tgaaattaag gaattattaa aggccataga
    10621 ctcaactaac ttagaatatg taaaacttga aagtagcgat ttaagattag aagtatctaa
    10681 aaaagctcaa tctacaagtt cacctgtttt aagtgtacaa caagaatcag tagtggatct
    10741 ttctttagaa aagccagttg taaacgatac acctgtcaca tctaatgaga atctatcagt
    10801 agtagtagct cctttgatgg gaacattcta tgattctcca agtccagatg cagatagctt
    10861 tgttaaagtt ggcgatgtag ttgaagaagg cgacacttta tgtatattag aagcaatgaa
    10921 gcttatgaat gagataacaa gtgaaattaa aggtgaaata attgaagtat tagttagtaa
    10981 tgaagaacta gtagaatata atcaaccttt atttaagata aaaccattgt agtaggaggt
    11041 atttaagttg ataaaaaaaa tactagttgc aaatagaggc gatatagcag ttagaatcat
    11101 cagaacatgt aaagaactag gtataaaaac tgttgctatc tattctgaaa tagataaaga
    11161 ttgttttcat agatatatag ctgatgaatc tatttgtata ggaccaaata atataagtaa
    11221 gagttataat aatatagaaa atataatcta tcttgctctt aaattaaagt gtgatgctat
    11281 tcatccaggc tttggatttt tatcagagaa tcctgaattt gcaaagcaat gtgaagataa
    11341 taatattatt tttataggtc caacaagaga acaaatgatt ttgatgggag ataaatctag
    11401 agcaagagaa actatgatgg aattgaatat ccctgttgtt cctggttcag aatctgtttt
    11461 gaaaactaag gaagaagctt tagaagttgc aagagaaatt ggatatcctg ttatgataaa
    11521 agcctcaagt ggaggcggtg gaaaaggtat gagaatcgtt agaaaagaag aagaactttt
    11581 ttcaaatttt gatatggcaa gttcagaagc tctagcagca ttttctaata gtgatttata
    11641 tatggaaaag tttatagaaa atcctagaca tatagaagta caagtatttg gagataaaca
    11701 ttcaaatgca atacatttag gtgatagaga ttgttctatg caaaggcgaa atcaaaaagt
    11761 aatagaagaa tctttaagtc catacctttc agatgaagaa agacaaaaac ttcataaaat
    11821 tgcagtagat atagtaaaag gagtaggtta tatcggtgca ggtactattg agtttatagt
    11881 tgacaaagat aaaaactttt attttataga gatgaataca agaatacaag ttgaacatcc
    11941 agttacagag atggtaacaa atcttgacct tataaaactt caaatatcta tagctaatgg
    12001 ggataaaatt ccttttaaac aagaagatat tacttttaga ggtcatgcta ttgaatgtag
    12061 aataaatgca gaagattcat caaagaattt tgctccatca ccaggtaaga tagaatcatt
    12121 aaatttacct ggaggttttg gtgttagatt tgatactttt gtatatgcag gatatactat
    12181 accaccttta tatgattcta tgataggtaa gttaatatgt tgggctgaaa ctagagaaga
    12241 atgtataagt agaatatata gagctctaga cgaaattatt gtagaaggta ttaatacaaa
    12301 tgtagaattt caaaaggcac ttgtaacaag tgaggagttt agagaagata ctcatcatac
    12361 caaatttata gaagatgtct ttatgaagaa agaatttgct acattataag gagtaaatag
    12421 attatgataa aaaaattttt atcatcaaaa gaatctaagt atgttacgat ttctttagat
    12481 gacaacttta aaaagaatag tgtagatgat aaattttgga cctattgcaa aggttgtgat
    12541 agtcatgtat ttagaaagga catagaagaa aattcatttg tatgtccaaa atgttcaaga
    12601 cattatggct taagagcaag aaaaagaata aatttattga ttgataaagg aacttttatg
    12661 gagtttaatt ctgatgtaga atttcaaaat ccactaaatt ttcctaaata caaagaaaaa
    12721 gtagactctt ataaggaaaa aacaaaagag tcagaagctg ttgtcacagg ttatggaaga
    12781 ataaatggaa ttaaaacagt tatttgtgta atgaatcctg attttatgat gggaagtatg
    12841 ggttctattg ttggggaaaa aataacttat tcaatagagt atgctgctga aaataattta
    12901 cctataatta tttgttctgc ttcaggagga gctagaatgc aagaaggtat ggtatctctt
    12961 atgcagatgg caaagacttc tcaagcactt tctaagcttg aagaaaaatt attgccatat
    13021 atatcagttc taactgaccc tactactgga ggagtaactg ctagttttgc tatgcttggt
    13081 gatattataa tttctgagcc aaatacgttg ataggttttg caggtccaag agtaatagag
    13141 caaacaataa atcaaaaact tcctgaagga tttcaaactt ctgagttttt acttgaaaaa
    13201 ggttttattg acatgatagt agatagaaga aaaatgaagg aagtattata tcaaattctt
    13261 gctatgcaca agaaataggg cataaaggag gaactgtttt gattgaaaat aatagtgaaa
    13321 aaataaaagt cttagaaaat gatattaaac aattaataac tatttctaag gcaaataata
    13381 tagatttaag tgataaaata aattcactta atgagaaatt ggaaaagctt aaggaagatg
    13441 ctttttcaca tctatcagca tatgaaaagg taacattaag tagagatata aagagaccta
    13501 cgacattgga atacatagaa catatttgtt ctaatttttt agaacttcat ggagataggc
    13561 tttttaaaga tgacccttct atggttggag gaattggtca gataggaaag tttaatgtta
    13621 ctatagttgg acaccaaaaa ggaagagata caaaagaaaa tataaaaaga aattttggaa
    13681 tgcctcatcc agaaggatac agaaaagcat taagacttat gaaacaagct gaaaaattta
    13741 atagacctat aataacgttt attgatacat caggagcttt ttgtggtcta gaagcagaag
    13801 aaagaggaca aggagaagct attgcaagaa acttacttga aatgagtaaa ctttctgttc
    13861 cagtaatcac ttttgtaatt ggtgaaggtg gtagtggagg agctttaggt atcggagtag
    13921 gaaatgatgt atgtatgtta gaacattcag tttattctgt aatttctcca gaaggacttt
    13981 ctagtattct atttaaagat tcatctaaag ctaaagaagc ttgtgatgtt atgaagctta
    14041 caagtaatga tttatatgat ttaaaaataa tagataagat tataaaagaa cctcttggag
    14101 gagcacaaaa agatgtagaa gctgtttcaa aagaaataaa agcttatatt ttagaaagat
    14161 taaatcatta caaagatatg gataaagagg aaattatagc tcaaagatat aaaaaattta
    14221 gaaatattgg aaaatgctta tagaatataa agaaatttgt agttttttca taataaaatg
    14281 gttaaaaaaa ataaaggttt tcttaaaacg attatagaag tatattttta taaaagtata
    14341 ctttaaatga tgattgtaat tagtaaatgg caattatgat ttagtacttt aggtaatgga
    14401 tactttaaca aattaacaag gagatgttga aatggaggta ctaaaagttt catcaaaatc
    14461 taatcctaat tctgtagctg gagccttggc tggagtacta agagaacgag gcgttgcaga
    14521 aatacaagct ataggagctg gagctctaaa tcaagcgata aaatcaattg caatagcaag
    14581 aggttttgtt gcacctagtg gaatggattt agtttgcatt cccgcattta ctgatataga
    14641 aatcgaaggt gataagaaaa cagccattaa acttataatt gaacctagat aatatatctc
    14701 tttattagaa tttaattaag ttttaaaggt tggctataga aataggacac tatagtgaac
    14761 cttttttact ttatatgttt tattttatgt aacttttaat gtataatctt aagtaaacaa
    14821 ataagattgg aggaaatata cattatgaaa ttaaaattat tggtagaaga gtatgcagta
    14881 tgtagattaa ataatgacag taaaatacca acatggatag atacagagaa gttttactct
    14941 ataactagaa ctgatgatga attaagtgtt gtatgtttaa ataataatat tccttctgat
    15001 gtcaagtcag aaaaggagtg gagaatactt aagatactag gtcctttaga cttttcactt
    15061 gtaggaattt tatctaagtt aagtagtttg ttagcagaca acaaaataag catatttgca
    15121 atttctactt atgatacaga ctatatctta gtaaaagaaa aagatataga aaatgcttgt
    15181 aaaattttaa gctgtaatgg atatgaagta gagtagttaa atacttttac atgaggactt
    15241 aacttatgat ttatataatc gaaattttaa aaataagtgt aataaatatt ttttgtttca
    15301 taggaatatt tattgtattt ggattgattt ttagcattac agaaaatcta aataataaat
    15361 ttatattttc atcttttgga aaaactggaa ttattgtaac tggagctata ggaacgtttg
    15421 ttcatgaatt gagtcatttg ataatgtgtt taattttcat gcataaaata aattcagtta
    15481 agtttttcag accaatagaa agtaaaaatg atggtatact aggatatgta agtcatagct
    15541 ataaaaaaga taatctttat caaagtatag gaaatttctt tataggaatt gcacctatta
    15601 taggtggaac aataatgatt attttaattt ttaagatatt actgccagat agttatatga
    15661 aagtgactca aaatatagat ttaaagttat atgtaagtat gattaataat ttaaatataa
    15721 atgggtttat acacattatt ttaggt
//
LOCUS       DACULQ010000092        15429 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.092, whole genome shotgun sequence.
ACCESSION   DACULQ010000092 DACULQ010000000
VERSION     DACULQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.092"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="JA154_11300"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="JA154_11300"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            179..253
                     /locus_tag="JA154_11305"
     tRNA            179..253
                     /locus_tag="JA154_11305"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Asn,seq:gtt)
     gene            259..344
                     /locus_tag="JA154_11310"
     tRNA            259..344
                     /locus_tag="JA154_11310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Leu,seq:taa)
     gene            360..435
                     /locus_tag="JA154_11315"
     tRNA            360..435
                     /locus_tag="JA154_11315"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Met,seq:cat)
     gene            443..517
                     /locus_tag="JA154_11320"
     tRNA            443..517
                     /locus_tag="JA154_11320"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Glu,seq:ttc)
     gene            532..605
                     /locus_tag="JA154_11325"
     tRNA            532..605
                     /locus_tag="JA154_11325"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Gly,seq:tcc)
     gene            611..686
                     /locus_tag="JA154_11330"
     tRNA            611..686
                     /locus_tag="JA154_11330"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:644..646,aa:Val,seq:tac)
     gene            692..768
                     /locus_tag="JA154_11335"
     tRNA            692..768
                     /locus_tag="JA154_11335"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:726..728,aa:Asp,seq:gtc)
     gene            779..853
                     /locus_tag="JA154_11340"
     tRNA            779..853
                     /locus_tag="JA154_11340"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:811..813,aa:Gly,seq:gcc)
     gene            863..936
                     /locus_tag="JA154_11345"
     tRNA            863..936
                     /locus_tag="JA154_11345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Arg,seq:tct)
     gene            1920..2759
                     /locus_tag="JA154_11350"
     CDS             1920..2759
                     /locus_tag="JA154_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAT7996448.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            2756..3937
                     /locus_tag="JA154_11355"
     CDS             2756..3937
                     /locus_tag="JA154_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996449.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            3934..4053
                     /locus_tag="JA154_11360"
     CDS             3934..4053
                     /locus_tag="JA154_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996450.1"
                     /translation="MNKYFEYLVVTRCDKKTYKIKFVNFMFLLHLVYFSYIIK"
     gene            4241..5143
                     /locus_tag="JA154_11365"
     CDS             4241..5143
                     /locus_tag="JA154_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HAT7996451.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            5165..6244
                     /locus_tag="JA154_11370"
     CDS             5165..6244
                     /locus_tag="JA154_11370"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086582.1"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HAT7996452.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            6234..6911
                     /locus_tag="JA154_11375"
     CDS             6234..6911
                     /locus_tag="JA154_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT7996453.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6954..7169
                     /locus_tag="JA154_11380"
     CDS             6954..7169
                     /locus_tag="JA154_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase"
                     /protein_id="HAT7996454.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            7198..8277
                     /locus_tag="JA154_11385"
     CDS             7198..8277
                     /locus_tag="JA154_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HAT7996455.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            8277..9029
                     /locus_tag="JA154_11390"
     CDS             8277..9029
                     /locus_tag="JA154_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HAT7996456.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            9030..9587
                     /locus_tag="JA154_11395"
     CDS             9030..9587
                     /locus_tag="JA154_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HAT7996457.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            9757..11103
                     /locus_tag="JA154_11400"
     CDS             9757..11103
                     /locus_tag="JA154_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAT7996458.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            11370..13202
                     /locus_tag="JA154_11405"
     CDS             11370..13202
                     /locus_tag="JA154_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HAT7996459.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            13653..14948
                     /locus_tag="JA154_11410"
     CDS             13653..14948
                     /locus_tag="JA154_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HAT7996460.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADNENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLIKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFTSNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNRQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
BASE COUNT         6001 a         1808 c         3055 g         4565 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc atttcgaaca cagaagttaa gctccctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg ttaaatgttc
      181 caaggtagct caacggtgga gcaaccggct gttaaccggt aggctgtggg ttcgagcccc
      241 acccttggag ccaaaaaagc cgaagtggcg gaactggcag acgcacagga cttaaaatcc
      301 tgcgggactt acctctcgta ccggttcgat tccggtcttc ggcacttata aaacaatatc
      361 gcggggtgga gcagttggca gctcgtcggg ctcataaccc gaaggtcgta ggttcaagtc
      421 ctgcctccgc aaccattaaa acggctcgtt ggtcaagagg ttaagacacc gccctttcac
      481 ggcggtaaca ggggttcaat tcccctacga gtcaccaatt caattaaata tgcgggtgta
      541 gctcaatggt agagttctgg ccttccaagc cagctgtgag ggttcgatcc ccttcacccg
      601 ctccaaaaat gggaccatag ctcagctggg agagcacctg ccttacaagc agggggtcac
      661 aggttcgagc cctgttggtc ccaccattcg cggcctggta gttcagttgg ttagaatgcc
      721 agcctgtcac gctggaggtc gagggttcga gccccttcca ggtcgccaaa ttaaataagc
      781 gggaatagtt cagtggtaga gcgcaacctt gccaaggttg aagtcgcgag ttcgaatctc
      841 gtttcccgct ccataataac ttgggtgcat agctcagctg gatagagcaa cgcccttcta
      901 aggcgtgtgt ccggggttcg aatccctgtg cgctcattaa gaaaagttta ctataaatta
      961 tagtaaactt ttatattgtt taaaaagaca caggaaacat gtatattacc tgtgtctttt
     1021 tatttataaa ctttaaacta tgtaaaaatt acagtatatt ctaatagagt tacagtatat
     1081 tcattaatat tgtaaacata cggagggtaa tgtataatag tctacaagat gtttatgaag
     1141 gcatcagtta aatataataa atataactaa ttagtaagag ttatatacat tatattgtat
     1201 actttatggt atttatatat tgaaataact tatgaaatta tttataaaga gattcatgag
     1261 catatttttg tctaaacaat aagtataaga aattataaaa agttagagac tatagtgaat
     1321 ttacaattta atgtaatatt tataagttat actaaataaa ttattatact gtgaaatgga
     1381 tatataactt taggtattgt gtattatttt cactgtagga gattatatct agttatacat
     1441 aataagtttt aatgatagtt tatagtaaga tattaaaata aatttatcta atctagatat
     1501 gaaataagtt attactttaa tataaaaatg aatctaatgg aataatattg atgataagta
     1561 atatttagat tttgtatttg tttatagtta ataagtggat tatagaattt ataataatta
     1621 atatatatta aacaaagata tattaagata ttttagaaca taaaactgaa ttaaggcgcg
     1681 agtactattt tgtattcgcg ccttgatggt ttgagttatt tgagtaaaaa ctaactttaa
     1741 taagaaatat gtatttatta agttgatatt tttaatgtgt ttagagatta tttaacaatc
     1801 tccaaatata aaactagaat tatttacata aaagttaaat agtttgtata gagttagatt
     1861 tatatagtat aatacttagt gtaaataatt aagaagttat caaacaggag gataaaactg
     1921 tgttagatat aaattctttt ttaaatggat tttttaatat aatattaaga atgagtatat
     1981 atgatttaat tgacatatct atagtagcgt atatttttta caaaatattt atgtttataa
     2041 aagatacaag agcagaacaa gtgtttaaag gtataatatt tcttttatta gcaactcaac
     2101 ttagtaacac ctttaaattg cataccgttt attggatatc tcttaaagct ttagattatg
     2161 gagttatagc tgctcttata atttttcaac cagaatttag agcaggactt gaacatatag
     2221 gaagagcaaa gtttaattta tttggaaaga atgtaaatac ttctgaagaa actctcaata
     2281 gaaatataga agaaattgta gaagctttat attcactttc tagacaaaaa ataggagctc
     2341 ttatcataat ggagagagaa actagaataa gtgatataat aaatacagga actattattg
     2401 atgctgaaat ttctaggcaa ctattaataa atatatttat accaaataca ccattacacg
     2461 atggagctgt tgtaattaga gattctaagg taaaagcagc tgcatgtttt ttgccattga
     2521 cagaaagtaa ggatttaagt aaagatttgg gcactagaca tagagcagga ataggggtaa
     2581 gtgaggtgtc tgactgtata actttgattg tatcagagga aacaggtggc gtatcaattg
     2641 ctaaagcagg taagttatat agagatattt ctagagaaag aatgatgaat atattacgta
     2701 gtaatttaaa aacaaataca gaaacaagaa gttttttcaa aggtggtata tttaaatgat
     2761 aaatagacta aaaaataata ccaaaataaa aataatatca tttttaagtg caattgcatt
     2821 atggatgtat gttatggctg tagttgaccc tgaagaaact aaagggtttg aagatatacc
     2881 tgtatctata agtaatatga gtgatttaaa ggataaaaat cttgttatat atccagacga
     2941 aaaattaaca gcagatgtat atataaaagg tagtttatca aaggtacaaa gtgttaaaaa
     3001 agaagatata catgtatatg gaaccattga agacccaata caaggacaga aggaagtata
     3061 tttaaaagca aatattcctc aaggtgttac tctagaattt aagcatgatg ttttagtagt
     3121 aaatcttgaa aaaaacataa aagaaaaaag agatataaag gtagaagtaa gtggaaattc
     3181 aaaaagaaat ataaataaaa ttgaacttag taaggaattt gtcgatgtat caggtccaag
     3241 aactctagtt aaagaagtca aaagtgtaag agctaatcta gatgttggta atgagacaga
     3301 tgatttttct aaaaaattgc aattaatacc attaaatagt gatggaacta aggttgaagg
     3361 agtaacatta agttcatcct ttgtgactgc aaagatagag ctattaaagg aaaaaattgt
     3421 cccaataaga gtaagtctaa atgataataa agataatgat gtactgaaaa actataaatt
     3481 aagtaaggaa caagttacaa taaaaggaaa gaaagaagat atagacaaaa taagttatat
     3541 taagacaaaa cctattgact tagctgattt ggctaatggt gaagaaaaag aagttagctt
     3601 agaaattcca gagggtataa ctcttgagga tagctctata acaattaaat taaatgatac
     3661 aaaacaattg agtgcagaat ttttatataa tgcgggagat attgaattaa gaaatatccc
     3721 aacaaatatg gataatccaa atgtaatttc tgatgtagat ataaaagttg ttgtagagtc
     3781 aacggaggat ttgagtaagt taaataaaaa agatatttca ttatatattg atttatcaca
     3841 aggtcctgag gaagatagta aatataaaat aaaatatgaa acaacatatc aatttaaaaa
     3901 aataaatata gaacctaata ttgttgaagt ggaatgaaca aatatttcga atatttagtt
     3961 gttacaagat gcgacaaaaa aacctataaa attaagtttg ttaattttat gtttttgttg
     4021 catcttgttt attttagtta tattataaaa taaggacaat gagatatttg ttaaatctaa
     4081 aaagcgctag atatatgaaa aaagactgat aaatagattt aaataaatat tttgaattaa
     4141 atatttcaat tatctctagg aaaacgttgc tgaacgagtt ataatttaat tgagatataa
     4201 aaagttctat ttcaaaaaat tcaagatgag gtggaaatgc atgagaagtt ttgaagaagt
     4261 aattaagttt gcaaaagaaa gaggacctaa aactatatca gtagcatgtt gccaagataa
     4321 agaagtttta atggcagttg aaatggctag aaaagaaaaa atagcaaatg ccattttagt
     4381 aggagatata gaaaagacta aagaaattgc aaaaagcata gacatggata tcgaaaatta
     4441 tgaactgata gatataaaag atttagcaga agcatctcta aaatctgttg aattagtttc
     4501 acaaggaaaa gccgacatgg taatgaaagg cttagtagac acatcaataa tactaaaagc
     4561 agttttaaat aaagaagtag gtcttagaac tggaaatgta ttaagtcacg tagcagtatt
     4621 tgatgtagag ggatatgata gattattttt cgtaactgac gcagctatga acttagctcc
     4681 tgatacaaat actaaaaagc aaatcataga aaatgcttgc acagtagcac attcattaga
     4741 tataagtgaa ccaaaagttg ctgcaatatg cgcaaaagaa aaagtaaatc caaaaatgaa
     4801 agatacagtt gaagctaaag aactagaaga aatgtatgaa agaggagaaa tcaaaggttg
     4861 tatggttggt ggaccttttg caattgataa tgcagtatct ttagaagcag ctaaacataa
     4921 aggtataaat catcctgtag caggaagagc tgatatatta ttagccccag atattgaagg
     4981 tggtaacata ttatataaag ctttggtatt cttctcaaaa tcaaaaaatg caggagttat
     5041 agttggggct aaagcaccaa taatattaac ttctagagca gacagtgaag aaactaaact
     5101 aaactcaata gctttaggcg ttttaatggc agcaaaggca taattttaag ggggaaatag
     5161 gcaaatgagc aaaatattta aaatcttaac aataaatcct ggttcgacat caactaaaat
     5221 agctgtattt gataatgagg atttagtatt tgaaaaaact ttaagacatt cttcagaaga
     5281 aataggaaaa tatgagaagg tgtctgacca atttgaattt cgtaaacaag taatagaaga
     5341 agctctaaaa gaaggtggag taaaaacatc tgaattagat gctgtagtag gtagaggagg
     5401 acttcttaaa cctataaaag gtggtactta ttcagtaagt gctgctatga ttgaagattt
     5461 aaaagtggga gttttaggag aacacgcttc aaacctaggt ggaataatag caaaacaaat
     5521 aggtgaagaa gtaaatgttc cttcatacat agtagaccct gttgttgtag atgaattaga
     5581 agatgttgct agaatttctg gtatgcctga aataagtaga gcaagtgtag tacatgcttt
     5641 aaatcaaaag gcaatagcaa gaagatatgc tagagaaata aacaagaaat atgaagatat
     5701 aaatcttata gttgcacaca tgggtggagg agtttctgtt ggagctcata aaaatggtaa
     5761 aatagtagat gttgcaaacg cattagatgg agaaggacct ttctctccag aaagaagtgg
     5821 tggactacca gtaggtgcat tagtaaaaat gtgctttagt ggaaaatata ctcaagatga
     5881 aattaaaaag aaaataaaag gtaatggcgg attagttgca tacttaaaca ctaatgatgc
     5941 tagagaagtt gaagaaagaa ttgaagctgg tgatgaaaaa gctaaattag tatatgaagc
     6001 tatggcatat caaatctcta aagaaatagg agctagtgct gcagttctta agggagatgt
     6061 aaaagcaata ttattaactg gtggaatcgc atattcaaaa atgtttacag aaatgattgc
     6121 agatagagtt aaatttatag cagatgtaaa agtttatcca ggtgaagatg aaatgattgc
     6181 attagctcaa ggtggactta gagttttaac tggtgaagaa gaggctcaag tttatgataa
     6241 ctaatgataa aagaataaga atcataactg gtcactatgg aagtggtaaa agtgaatttg
     6301 cgatgaacta tgttgttaag ttaagagata tggtttctgg caaagttgct atagccgatt
     6361 tagatgtggt aaatgtatac tttagaacta gagaaaagaa agagttaatg aaatccttag
     6421 gtatacaacc aatagatagc tcaattaatg ctccaacatt agatttacca gcagtatcag
     6481 cagaggtaat gtctcctatg gtagaccatt catacaattc tgttatagat ttaggtggag
     6541 ataatgtagg tgctagggtt ataggaagat ttagtcattt gttaaaagaa ggcgattatg
     6601 atatgctttt tgtaataaat gcaaataggg agaaaactca aacttctgaa gaagttatcc
     6661 aatatataaa agaaatagaa aaatcttcta aattaaaggt aacaggtcta attaataata
     6721 ctcatcttat taggtttact actattgatg atgttttaag aggtcagaaa gttgcaaaag
     6781 aagtttctga aaaatgtaat ataccgataa gatatgttgc atgtttagaa aatcttgttg
     6841 aacagttacc aaaagattta gaaggagaaa tttttcctat aaaattatat atgagagaag
     6901 actggatgta gtttgagctc aaatatatag aaagtttaag gaggcttagg taaatggcta
     6961 aaggaaaagt atcttttaat caagaacgtt gtaaaggttg tggattgtgt gttgaagcat
     7021 gtccagtaaa aatcatacaa ttagactcaa atgtaataaa caaaaaaggg tataacccag
     7081 caactgtttt tgaaatggaa aaatgcatag gatgtgcaag ttgtgcaact atgtgtccag
     7141 atgtagttat aacagtagaa agagattaat ttaattaata tttaaggggg aattttaatg
     7201 gctaagatac ttatgaaagg taacgaagca tttggaaaag cagctataga agctggttgc
     7261 aaatatttct tcggttaccc aataactcca caaagtgaat taccagaata tttatcaaga
     7321 gagttaccta aaataggtgg agcgttcgtt caagctgaat cagaagtttc tgcgataaac
     7381 atggtttatg gtggagcagg tgcaggagca agagttatga cttcttcatc ttcaccagga
     7441 gttgctttaa agcaagaagg tataacatat gcagtaggag cagaagttcc ttgtgttgta
     7501 cttaatgtaa tgagaggtgg tccaggactt ggaagtatac aaccttcaca agctgactac
     7561 tttatgtcta caagaggtgg aggaaatgga gactacagaa ctccagtatt cgcaccagct
     7621 acagttcaag aagctgttga tatgataatg gaagcttttg acgttgcaga ttattacaga
     7681 tcaccagtta tggtagttgc agatggtatg ataggtcaaa tgatggagcc agttgaattt
     7741 agagctcctg agaagaaaag agaattgcct cctaaagatt gggcaactgt tggaactaaa
     7801 ggaaaaagaa aacctaatgt aataaactct ctatacttag agccagaagt actagaagac
     7861 cattgttggc atttacaaga aaaatttgat gcaatggaaa agaatgaagt acaatacgaa
     7921 atgtacaaaa cagaagatgc tgaatttgta tttgcagctt atggaactac ttcaagagta
     7981 gtaaaaagcg caattgatat attaagagaa gaaggaataa aagcaggtct tataagacct
     8041 aaagtattat ggccattccc attcgaagca ttcaaccaaa ttcctaatgc tagaaacata
     8101 ttgactgttg aaatgagtat gggacaaatg gtggaagatg ttaaaatggc agtagaaggt
     8161 aaattaccag tttacttcca tggaagacca ggaggaatga ctccaacacc agcagaaata
     8221 gttgaaaaag ctaaaaaaat tattgccgga gaattggttg caggaggtgc tagataatgg
     8281 ctgttgtatt taagaaaact gaaggattac aagatactca aactcactac tgtccaggat
     8341 gtacacatgg tataatccat agattagttg gtgaagtatt agaagaacta ggagtattag
     8401 gtgatgctgt aggtgttgtt ccagttggat gttcagtttt aggatataaa tattttaact
     8461 gtgacactca agaagctgct catggtagag cgccagctgc tgctacagga ataaaaagag
     8521 ttcatcctga aaatacagta ttcacttatc aaggtgatgg agacttagct tcaattggta
     8581 ctgctgaaat agttcatgca gctgctagag gagaaaaaat aactactata tttgtaaaca
     8641 atacaactta tggtatgact ggtggacaaa tggctccaac tacattagtt ggacaaagag
     8701 ctactactgc tcaatcagga agaaatgctg aaactcaagg ttatccaata agagtaagtg
     8761 aaatgttagc tacattaaca ggagctgtat ttgtagaaag agtagcagtt gatactccag
     8821 ctcatgttag acaagctaaa aaagctataa agaaagcatt ccaagttcaa caagcaggat
     8881 taggatttgg aatagtagaa gtattatcta cttgtcctac aaactgggga ctagctccaa
     8941 acgatgcttt acaatggtta agagacaaca tgataccata ctatccatta ggaaatttca
     9001 aaaatgttga agttgaggag gtgaagtaaa tgtctacagc tagagtaata tgtgcaggct
     9061 tcggaggcca aggtgttatg tctatgggac aattacttac ttatgcagga atgctagaag
     9121 gaaaagaagt ttcttggtta ccatcttatg gaccagaaat gcgtggaggt acagcaaact
     9181 gtgcagtaac tgtatctaat gagccagttg gttctccact tataacagat gatgcaactg
     9241 cagctatatt attaaatata ccagcatttg aaaaattcaa agatgatgtt attcctggtg
     9301 gaaaaataat agtaaatagt tctttaataa aagaaaaagt tgatagaaca gatgtagatg
     9361 tttattatat accagcaaat gaattagcag ctgaattagg aaatgataaa gttgctaaca
     9421 tgataatgtt aggtgcttac ttaaaagtat ctgatactgt agatatagaa tctgtactag
     9481 aagcattcaa aaaagttttc ggacctagaa aagaaaaatt cgttccatta aacagagaag
     9541 ctttacaaaa aggtatggat gctacatgtt ctaaagcaac taactaataa agaaaaagat
     9601 gtcaaaaaaa agacaataga attatttcta ttgtcttttt tgctgataag tattaaaata
     9661 tttttatgac tgaaattaag cctataatga tattttatga actataaaaa tataatacaa
     9721 attacaagct tttcaattat gaaaggagaa tctttagtga gaaagtattt tggaacagat
     9781 ggagttagag gagtagcaaa tacagaacta acatgtgatt tagcatataa acttggtaga
     9841 gctggtggat ttgtcttagc tcaaggagac cacagagtaa aagtagtagt aggtaaggat
     9901 actagaatat caggtgatat gttggaagca tctttaatag caggacttat gtctgttgga
     9961 tgtgatgtta ttactgttgg aataatacca acacctgcag tggcatattt aactagaaaa
    10021 tatggtgctg attgtggtgt ggttatatct gcatctcaca atcctgttga atataatgga
    10081 atcaaattct ttaataagaa tggatataaa ttagatgatg aaattgaatt gaaaatagaa
    10141 gaatatatag acgatataga caaaattgat tgtcttccaa ttggtgaaaa tgtaggaaga
    10201 aaattacatg aacattgtgc acaaagagac tatgtagact acctaaaatc aataataagc
    10261 actgacttta agggattaaa agtagttttg gactgtgcta atggtgcgtc atataaagtg
    10321 gcaccaatag tgtttgatga actaggagca agtgtaattt caataaatag ttcaccagat
    10381 ggaaataata taaactataa atgtggctct acacatccag aacaacttca aagggctgtt
    10441 ttggaacata atgctgactt aggtcttgca tatgatggag atgctgatag attaattgca
    10501 gtaaatgaaa agggtcaaat tgttgatgga gaccatataa tgatattgag tgccttaaat
    10561 ttaaagaaaa ataataaatt ggcacaagat actttggtag ttacagttat gagtaatata
    10621 gggttgacaa tagctgctaa ggaaaatgga ataaatttat ctactacagc agttggagac
    10681 agatatgttt tagaagatat ggttaaaaat ggatataatc ttggtggaga gcaatctgga
    10741 catatgatat ttttagatta caacactact ggagatggag tgcttagttc tctaatactt
    10801 gcaaatataa tcttgcaaga aaagaaacct ttatcagaaa ttgcatcaat aatgagccaa
    10861 tacccacaag tattagtaaa tgcaacaata aaaaatgaaa ataagaacaa atatatggaa
    10921 tatccagaaa taaaaactga gatagaaaga atagaatcta ttttagacgg aaatggtaga
    10981 gttttgataa gaccatcagg aactgagcca ttagttagag ttatgttaga gggtaaagag
    11041 gaaggtcaaa taaaagaatt agcaacaaac ttagctaatc ttatacaaga aaaattatcg
    11101 taatttaaag ggtattctca gatataaata ctgaggacac ctttctaagc gccagaactt
    11161 agttatagac ataattattg agtaatatag gaactaagtt gacgaggtca gagttaatcg
    11221 aattgtcggc ggatgctctg cggtgtgttt accatcgaaa gacttcttta aataatagga
    11281 gcaatcctat ggacaaaggg aagccaaagt aaacttatat gacctaactc ttaaaatata
    11341 taaataatat ttttaggagg acacaaataa tgtgtggaat agttggatat ttaggaagta
    11401 gaaaggcagc agaagttata gtagaagggc tttctaagct agagtacaga ggttatgact
    11461 cagcaggagt agcagttaat agttctaatg aaaaagaact taatattaga aagtttaaag
    11521 gtagactttc tgtacttgca gaagacttag aaaagaatcc tattgatgga aatttaggta
    11581 tagggcatac gagatgggca actcatggag aaccatctga tgtaaactca catcctcatt
    11641 ttaatcaagc caaaacaata gcagttgttc ataatgggat aatagaaaac tatatggaaa
    11701 taaaagaaga attaatttct gaaggtgtta agtttgaatc tcaaacagat acagaggtaa
    11761 tagcacattt agtagataaa tactatgagg gtaatttatt agatgctgta tacaaaacta
    11821 tatcaaaatt aagaggagct tatgcattag gtgttatatg taaagaacat ggaaatgaat
    11881 tagttgcagt aagaaaagat agtcctcttg tagttggtgt tggtgaaggt gaaaacttta
    11941 tagcatcaga tatacctgca ttattaaaat atactagaga tgtttatttc ttagaaaatg
    12001 gagaagtagt tcatctaaaa gacgaaaatg taacagttta tgactctaat agaaatcttg
    12061 ttgaaaaaga agtatttcat gtaacttggg atgtagaagc tgcgtctaaa ggtggatatg
    12121 actactttat gtcaaaagaa attcatgagc aaccaacagg agtaagagaa actttagaaa
    12181 gaagacttga tgataatgga aatataattc tagacagtat aaatatttct aaagaagact
    12241 tagaaaaaat aaataaagtt tatatagtgg cttgtggaac agcatataat gctggattac
    12301 ttggtaagta tgccatagaa aaatttgtaa atataccagt aataactgat atagcatcag
    12361 aatttagata tagcgaccca tttgtagatg aaaattcatt agtaatatta gttagtcaat
    12421 ctggtgagac agcagatact ctagcagtat taagagattc aaaggctaaa ggagcaagaa
    12481 tactttcaat aactaacgtt gtaggttcat ctatagcaag agaatcagat gatgtattct
    12541 atacttgggc aggacctgaa gtagcagttg catcaactaa ggcatatact actcaaataa
    12601 cttctttata catgatagct ttagactttg ctatcaaaaa aggaacaata actagagaat
    12661 tttatgatag tatgatttct aagatgaaag aaataccatc aaaaatacaa gaaatactag
    12721 acaatgaaga atatataaaa gaagttgcta aaacagttgt tagttctgaa catgcattct
    12781 atttaggaag aggtatagac tatagcttag caatggaagg ttcattgaaa ttaaaagaga
    12841 tttcatatat acatgcagaa gcttttgcag caggagagct aaagcatgga acaatagctt
    12901 taatagaaaa aggaactcct gtaattgcca tagctactca agaaaagtta tttgaaaaaa
    12961 tggtttctaa tatggaagaa gtaagagcaa gaggagctta tgtagtagca atagctcaaa
    13021 gccataataa ggatgttgag aaagctgctg ataaaataat ctatatacca aattcagatg
    13081 atattttatc accaatatta gcagttgtac caatgcaatt acttgcttat catgtctctg
    13141 tactaagagg atgcgatgta gataagccaa gaaatctagc taaatcagta acagttgaat
    13201 aaaaacagat taaaaattaa atagataata tttattgaaa agaactcatc ctgttatttg
    13261 tattttatag gatgagtttt ttaaataaat attaaatttg ttttgttttt gctataataa
    13321 attttataaa aagattgata ttaaatcatc tgtaaaatga taaatatata gaccatacca
    13381 ggtataggga acgataaata tagttcatat aaaatatagt gagtacttaa taaacatata
    13441 gtcaatatca gataaaaaaa ataatagctt atgagaaaaa ttaaaataca aggtattagt
    13501 aaccttgtga taagataaag tgagtagttt aaaataagaa tataaggaac tatttataaa
    13561 agaatataat gaagctaata aagtataaga aaattacttt attttagtaa aataatttca
    13621 aataaaaatt ttttaaattg ggagtataaa ttatgacaaa tgattttaaa aaagaaaaga
    13681 aagaagatta ttttgttaat cttaaggcaa tatctactga agttttacaa gaaaaagtgc
    13741 aagataacgc ttgggttata gttgatactc gcttaaatga tgcttataat ggctggaagt
    13801 tagatggagt aaaaagaggt ggacatataa aaggagcagt agatttttct gctaattggt
    13861 taagtgtata ctcagataga aaagatgaag tgctagaaca agcattgaaa actaaaagaa
    13921 ttgatttaga taaaaatata gtattatatg atgcaaatgg aaaagatgct ttggttgtag
    13981 cagattattt atctaaaaaa ggatataaat atctatataa atatgatatt aaacaatggg
    14041 cagacaacga aaatcttcca atggaaagat ataaaaatta tcaaatgata gttcctgctt
    14101 ttataataaa agatatatta gatggaaaaa ttcctgagac atttgaagat tcaaagaata
    14161 taaaaatgat tgaagcgagt tggggagaag aatcgtatac aaaagggcat atacctacaa
    14221 gtgttcatgt aaataccgat attatagaac ctcctccaac atggatgctt gataatgatg
    14281 ataacttaat taagtttgct ttagactatg gattaacaaa ggatgatact gtaatagttt
    14341 caagtagtac acctatggca tcttatagac tagctgttat attgagatat ataggagtaa
    14401 aagatgtaag agttttgaat ggtggaacaa actcatggtt atcagcagga tatgaattag
    14461 aatttactag taatccaaaa cattcatgta ctaattttgg agcagatata ccagttaact
    14521 cacaattagt tgttactacc tctgaattaa gacaaaaact aaaagaaaaa aataagttta
    14581 tactagtaga taaccgtact tgggatgaac atataggaaa agtatctgga tatacatatt
    14641 atgataaaaa agggcgtata ccaggagctt tatatggaca ttcaggtagt gatagtgttt
    14701 cactagaaga atatcgtaat attgataata caatgagaaa taaatatgag attttagaaa
    14761 tgtgggacaa ggaaaatata gatgtgaata gacagcttat atttatgtgt ggaagtggat
    14821 ggcgtgctgc tgaagtatta acatacgcaa atgtcatagg agtggaaaat acctcccttt
    14881 atagtgatgg atggatgggc tggagtcttg ataattcaaa tttaattgaa gtgggagaac
    14941 acaaataaag tttttgaaag tacgatagag ttgtttaaaa caaaggtgtt tttgatttat
    15001 tgggaagagt gtgtaaattg tgttttggga agtgtactta ggtatgttca tttggagcta
    15061 tggaaattga taaaaaagta ttatctgttt attttgctgg taaaatgtga atagattata
    15121 gattatagat tatagattat agatttaagt atagactaag taatttatat tacagaagag
    15181 aatatataag aactagtgca agatgtattt gatacatata aaaaattggc aaatccagaa
    15241 taaggataga gaaaattgag attgaagagt ttgaaagaga cttattagat gaaaaataca
    15301 aagatgtata aaaatacata ttgagaaagt atatctctct taaatttgtt aataattcta
    15361 ttatacagaa aaacatattt aagggggatt ttttactatg aaaagagtaa aattagctat
    15421 aagcttttt
//
LOCUS       DACULQ010000093        15272 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.093, whole genome shotgun sequence.
ACCESSION   DACULQ010000093 DACULQ010000000
VERSION     DACULQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.093"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..2183
                     /locus_tag="JA154_11415"
     CDS             <1..2183
                     /locus_tag="JA154_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAT7996461.1"
                     /translation="LIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIV
                     EKVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLES
                     DKIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITL
                     EFKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEV
                     YMEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKA
                     VKAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNN
                     ITLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFS
                     LKLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGR
                     CHYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGA
                     LDEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLI
                     TQVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNM
                     LSVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQI
                     LFKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            2201..2641
                     /locus_tag="JA154_11420"
     CDS             2201..2641
                     /locus_tag="JA154_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAT7996462.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            2656..3585
                     /locus_tag="JA154_11425"
     CDS             2656..3585
                     /locus_tag="JA154_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAT7996463.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            3595..4347
                     /locus_tag="JA154_11430"
     CDS             3595..4347
                     /locus_tag="JA154_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HAT7996464.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTVVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            4416..5117
                     /locus_tag="JA154_11435"
     CDS             4416..5117
                     /locus_tag="JA154_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAT7996465.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            5179..6504
                     /locus_tag="JA154_11440"
     CDS             5179..6504
                     /locus_tag="JA154_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAT7996466.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            6512..7543
                     /locus_tag="JA154_11445"
     CDS             6512..7543
                     /locus_tag="JA154_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089096.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAT7996467.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            7563..8315
                     /locus_tag="JA154_11450"
     CDS             7563..8315
                     /locus_tag="JA154_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAT7996468.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            8332..10347
                     /locus_tag="JA154_11455"
     CDS             8332..10347
                     /locus_tag="JA154_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HAT7996469.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            10455..11192
                     /locus_tag="JA154_11460"
     CDS             10455..11192
                     /locus_tag="JA154_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAT7996470.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            11290..12174
                     /locus_tag="JA154_11465"
     CDS             11290..12174
                     /locus_tag="JA154_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089092.1"
                     /note="EngC; RsgA; CpgA; circularly permuted GTPase;
                     ribosome small subunit-dependent GTPase A; has the pattern
                     G4-G1-G3 as opposed to other GTPases; interacts strongly
                     with 30S ribosome which stimulates GTPase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAT7996471.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            12179..12850
                     /locus_tag="JA154_11470"
     CDS             12179..12850
                     /locus_tag="JA154_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAT7996472.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLSDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            12859..13500
                     /locus_tag="JA154_11475"
     CDS             12859..13500
                     /locus_tag="JA154_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAT7996473.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            13631..14260
                     /locus_tag="JA154_11480"
     CDS             13631..14260
                     /locus_tag="JA154_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAT7996474.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            14315..14971
                     /locus_tag="JA154_11485"
     CDS             14315..14971
                     /locus_tag="JA154_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HAT7996475.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT         6164 a         1504 c         2933 g         4671 t
ORIGIN      
        1 atttgatata tccaaaggga tacaaattaa ataattataa agtagtccta ttaggtgaaa
       61 gtttaagtgg attaaatgat attgaattga aggaaaaaat atcaacttta agtgataaaa
      121 atagagaaat agtagagaaa gtgattgata gtaaaggtaa aattaaggtt gataagttaa
      181 aatatatacc aaacttaaat agttctttgt acacaatgaa taaaaatgga attataaaat
      241 tatgttggga atataaaaat cacaaaaatg aaaagaaagt atgttatata tctttgagtt
      301 tagaaagtga taagattgac gattatatag agcaaaataa aataaatgtg ggaagtaaac
      361 aaaaagagat attaagtttt cttaaaaata atgaaaatgt agagataaat gatttattgg
      421 atttattaaa tgcttcaaaa caaagtataa attctctaag taagaaaaag ctaataaccc
      481 tagaatttaa ggattattat agagagccaa aaagtattta caaatccgta ctaaaaagta
      541 taaaattaaa caatgagcaa caagaagcag ttgatgagat aaagtcgaat atgtttgtag
      601 atgataaaaa accatactta attcatggag taactggaag tggaaaaact gaagtttaca
      661 tggaaataat agaatttgct ctaaatcaag gcttagacag tatattctta gtacctgaga
      721 tagcattaac tcctcaaaca atagataggc taaagagtag gtttggagat ttagttggag
      781 tgttccacag taaactatct gaaggagaaa aacatgacgt ttacaaagct gtcaaagcag
      841 gtaaagtaag agtgttaatt ggagctaggt cagctttatt tgcacctttt aattccttag
      901 gcttaataat aattgatgaa tgtcatgagt catcatataa gtctgagaag aatccaaaat
      961 ttaatgctat agaagttgct agatttatgg cattaaaaaa taatataact cttattttag
     1021 gttcagcaac gccctcaatt gaagagtatt atagggcaaa atctggagaa tacaaactta
     1081 taaatataaa aagtagagca aatgataagc ctcttccaaa cattgaagtt gtagacatga
     1141 aagatgagtt agataaagga aatagaagta tatttagttt gaaattacaa aaagaaatac
     1201 gttatgcgat tgaagaaaat aatcaagtaa tactattttt aaacagaaga ggttatgcaa
     1261 attttgtatc ttgtagaaag tgtggatatg tgtttcaatg tgaaaattgt gatatatctt
     1321 taacttatca taagaaaagc aatactggaa gatgccacta ttgtggatat gagaaagaaa
     1381 ttccaaaaga gtgtcctgaa tgtaaaagta cttatgttaa gccatttgga gttggaactc
     1441 aaaaaattga agaggaatta aaatatattt ttccagatat aaaaactttg agaatggata
     1501 aagatacaac ttcaaaaaaa ggtgctttag atgagatact aaataaattt aaagataaag
     1561 aagcagatgt acttattgga acacagatgt taagtaaagg gctggatttt gagaatgtaa
     1621 ctttagtagg tattttgtct gcagacatga tattaaattt tccagacttt aaaagttttg
     1681 aaactacatt tcaattgata actcaagtct ctggaagagc agggagagct gataaagagg
     1741 gaaaagtagt acttcagact tatgacactg aacactatgc tattaagcat gctattgaat
     1801 atgattatga gggtttttat gaagatgaga taaaaataag aaaagcattt ggatattctc
     1861 cttttaataa tatgttaagt gtagttgtta gtggtgaaga tgaaagatta gtgataaaaa
     1921 atataaaaaa tatgcatgca tcattgattt atttattaga aaaaaggggt ataaatgatt
     1981 taggatttat attaggtcca aatccatgtt caatatcaaa aataaatcaa aattatagat
     2041 ggcaaatact ctttaaagat gaaaatattg aaattaatct cttaaagggt ataataaaat
     2101 atatatgtat aacaaaacga gatttgatat ttgataagaa tatcaatgta tctatagata
     2161 ttaatccaaa cagtgtatta taaatttagg aggaattata atggctttaa gacaaatagt
     2221 tcaaatagga gaaccagtat taagaaaaaa atcaaaaaag gttgaaaaaa ttgatgaaaa
     2281 gataatacaa ttattagatg atatggcaga aactatgtat gatgcagatg gtgttggact
     2341 ggctgcacct caagttggaa tattaaaaag agtagtagta atagatattg gagaagagct
     2401 tatagaactt ataaatcctg aaataataga aacatctggg gagcaaatag atgaggaagg
     2461 atgtttaagt gttgttggag aagcgggaaa tgttagaaga ccaaattatg taaaggtcag
     2521 agctttaaat agaaatggtg aaacaataga gttagaggga gaagaacttt tagctagagc
     2581 attttgtcat gagatagacc acttagatgg aattttattt gttgacaaaa tagaaaagta
     2641 gtaggagttg atttgatgaa aatagtattt atgggaactc ctgatatagc agttccatgt
     2701 ttacaaaaaa taatagatga gaagtatgaa atattgggtg ttgtaactca accagataaa
     2761 ccaaaaggaa gaggtaaaaa actaggtatg agtccagtaa aagaactggc aattgaaaat
     2821 aatatacctg tatatcaacc agtaaaggct agagataaag aatttataga taaaattaaa
     2881 tctttaaatc cagatgtaat agtagttgta gcttttggac agatacttcc aaaagaaata
     2941 ttagagattc ctaaacttgg atgtataaat gttcatgttt ctttacttcc aaaatataga
     3001 ggtgcggcac ctataaattg ggtaataata aatggtgaag aaaagactgg tgttacaact
     3061 atgtatatgg atgaaggatt agatactgga gatatgatac taaagacaga agtaaatctt
     3121 gatgaaaata taacagcagg agaacttcat gataaaatga tgaatatagg agcagaaact
     3181 ttaaaagaaa cattaagatt aattgaagaa ggaaatgctc caagagaagt acaaaatcat
     3241 gaagaatttt cctatgcgcc aataatgaat aaatcattag gtaacataga tttttcaaaa
     3301 agtgccagag aaatacacaa tttagttaga ggagtaaatc catggccaag tgcttatact
     3361 acctataatg atgtcataat gaaagtttgg aaaactaaag tgttagatga aaaaagcaca
     3421 aaagatgtag gtacaataat tgatgtaagc aaagatggga taaaagtaag tactatagat
     3481 aatgtacttt taattgaaga aattcagatg ccgaataaaa agagaatgtt ggttggtgaa
     3541 tatataaaag ggaatactat agaaactggt ttagtattgg gttaggtgaa aagtatgaca
     3601 gatataaaga agtatttaac aagtgttggg attttgattt taatattagg aattttgata
     3661 ggagttgtta atttttttat aataagttta actcaaatat tctccttgat gataaatatt
     3721 ataataatat cactaatact tataatacta tgttcaacag tagttactta tagagtaatt
     3781 aaaggaaagt atgttaactc aaatataatg aaaataaatt ttaatattgt cagtggatta
     3841 tttcctatta tatctttcat tgcatcatca tttggtatgt caaaaagtga tattagaaga
     3901 atatatataa agttaaataa tgaatatata tacagtaata aatataattt taatccagag
     3961 gatataataa ttttaatacc tcattgtatt caagagaata gttgtaaatt aaaagttact
     4021 aatgatatag ataattgtaa agaatgtgga agatgcaaca taggagaatt aattaagcta
     4081 aaggaaaaaa ctaatgtaaa aatatttgta gctacaggag gaactctagc tagaaaaatt
     4141 ataatggaca ctaaacctaa agctgttgta gctgttgcat gtgaaagaga tttgacatct
     4201 ggaatacaag atattaagaa aattcctgtt ttaggtgtat ttaacaaaag acctaatgga
     4261 ccttgtgtaa atactaatgt agacatgatg gatattgaaa aagctatagg ctttttaaca
     4321 ggaaaaaata tattagtttg taattaaact gtttttaagt taagtgaata ttaagaggat
     4381 gtataataaa aatatatttt aggagagtgt tatttatgta ccctgtatat ggcggttttt
     4441 ggggatttga cccaacaatg gttgtattaa ttccagctat attacttact atatatgcac
     4501 aatttaaagt gagttcaaca acaaataagt atttaagagt aaatacacgt cgaggatata
     4561 ctggagagca aacagcaaga agagtacttg attcaaatgg tttgtatgat gtaaagatag
     4621 aaatggttag agggcatcta agtgaccatt atgacccaag aagaaaggct gtaaggttgt
     4681 cagaagatgt atattatgga acatctataa cttctgttgc agtagctgca catgaatgtg
     4741 gtcatgccat acaacatgct aaaggttatg cacctcttca aataagaagt agtttagtac
     4801 cagtagtgaa ttttgcttca agtatatctt ggtttttaat atttttagga tttattatgg
     4861 ctggtccatt tttaaaaatt ggtatactat tgttttcggc ttcagtgtta ttccaaataa
     4921 taacattgcc tgtagaattt aatgcttcaa gtagagcaat cgttcaactt gggaatttag
     4981 gtattataga tgaaagtgag tcaagacaaa gtagaagagt actttcagca gcagctttaa
     5041 catatgttgc agcagcattg gtttcaatac ttcaattact tagattgtta ctaatagctc
     5101 aaagaagaaa tgactaaaag taaagtgggt tgtttagaaa caaccctttt ttatgtttaa
     5161 taaaagggag aaatttatat ggatgcaaga gaagttggat ttaaagtatt atgtgatata
     5221 gaaaaaaatg acaattattc aaatattgca ataaataagc attttaaaaa tttagaaata
     5281 agtgatatgg atagaggact tgcaacagag ttaatttatg gagtaataga aaataaatat
     5341 tatttagatt atataatcaa taaactttca aaaataaagg taaaaaaaat gtcaacttat
     5401 gttaaaatct ttttaagaat gggaacatat cagattttat ttttagatag tatatcagat
     5461 tatgcagcag ttaatgaaac tgtaaaatta tctaagaaat atgataaaaa atcttctggt
     5521 ttcataaatg ctattcttag aaatgaaata agagctaaag agactataat ggacataact
     5581 gaggaagata gtgtaaaata cttatctata aagtattcct ataactcttg gattataaaa
     5641 aactggatag ataaatttgg acaagaattt gctgaagatt tgttggaagc aaataatgaa
     5701 aagcctagta tctatataag aacgaataca cttaaaataa gtagagaaga acttattgaa
     5761 aaattaagtg aaatgggtat tgtatgttta aaagttccta tggtagaaga agctattaaa
     5821 gtcgaaaaaa tgaaaaatat agaaaaaaat gaattattta aggctggttt atttacaatt
     5881 caagatataa gttctatgat agtagggaaa gtaattaatc caaaagaagg ctcatttgtt
     5941 ttggatgtat gtagtgctcc tggagggaaa tctactcact tagctacttt aatgaacaat
     6001 actggtcaag tagtagctag agatattttt gagcataaac taaagcttat aaactctact
     6061 gtaaatagat tggggcttaa aaatgtgttt gtagaaagat ttgatgcttc tgagattgat
     6121 gaaaatagta taagtaaatt tgattatgtt ttagcagatg ttccttgctc tggttttggt
     6181 ataataagac gtaaaccaga aattaagtat aagaaagaag aagagttaaa agacatcact
     6241 tctatacaaa agaaaatttt agaaaatgca tctaaatatg tcaaagttgg tggaacttta
     6301 gtatatagta catgtacaat tcaagatgtg gagaatataa atatagttac ttcttttgtt
     6361 gaagaaaatc acaactttga atttgttcca attgatacag taaatgtaga cttagacaat
     6421 caagataaag gatatttaaa gatatatcct aatattcatg gtatagatgg ttttttcata
     6481 gcaaaattaa agagaataag gtagtgagag aatggaaaag aaaaagatag tattaaaaaa
     6541 ttttactgaa gatgaactta aagaatttat gaaaactata gatgaaaagc cctttagagg
     6601 aagtcaaata ttctcttgga tatataaggg tgcaaaaact tttgatgata tgaacaatat
     6661 accaaaaagt ttgagaaata aattagaaga ggtttcatgt atagggcata tagatataga
     6721 attaaagtta gaatctaagg tagataatac aaaaaaatat ttgtttttat tagatgatgg
     6781 aaatataata gaaactgtga tgatggatta tgatagtaga gttactgtct gtgtttctaa
     6841 ccaagtaggt tgtaggatgg gatgtaattt ttgtgcatct acaatggatg gattaattag
     6901 aaacttagag ccatgggaaa ttttagacca agttattaaa attcaagaag atactggtaa
     6961 gagggtatca aatcttgtat tgatggggag tggtgagcca cttgataact ttgaaaatac
     7021 aaaacaattt ttaaaaataa ttaacgaaaa aaatggtctt aatatagggt ataggcatat
     7081 aactctttca acttgtggaa tagtacctaa aatgtatgag ttggcagact tagaaatagc
     7141 tataaattta gctttatcac ttcattcacc atatgatgaa gaacgaagaa aaattatgcc
     7201 tgtagcaaat gcttattcta ttgaagaaat attgaatgca tgtagatatt atataaaaaa
     7261 gacaaataga agggttacat ttgaatattc tcttataaaa ggggtaaatg actcagaaaa
     7321 agaagcaaag gcattagcta agctcctaaa aggtatgcta tgtcatgtaa atttaattcc
     7381 aataaataaa gttgaagaaa gagaatatga aaagccagat aaagcattta tttataaatt
     7441 tagggatagt ttggagaaga acaatatacc tgccacagtt aggatgtcta tgggttctga
     7501 tattagtgga gcttgtgggc aattgagaag aaaatacaag taatttgtaa ggaggaagtc
     7561 gtatggttta tagttgtgcc tcccatatag gaaaaataag aaaaaacaat gaagactatt
     7621 gtgagggaga agttatagat acagaacatg gtccaatagg aatatttgcc atagctgatg
     7681 gaatgggtgg acataaaaaa ggtgaagtcg ccagtaaatt agcagtagaa aatataattg
     7741 attttctaaa ggaaaactta ttacaacatg ataatgtaaa aatagactat atagatgata
     7801 tattaaaaca ggcttataat aatgtaaact caattgtgca caaaaaatct atggaagata
     7861 tagaatttga aggaatggga actacattag ttacggctat agtgtataat aatgtcttat
     7921 atgtggcaaa tgttggagat agcagatgtt atttgttaac agatgaaaaa tttgataaaa
     7981 taactataga ccattccgtg gttgaagaac ttatgatggc ccatgtcatt acagaagaag
     8041 aggcaagaag acatccacag aggaatagaa taactagagc aattggaaca gatgatatgg
     8101 ttgttgtaga tatttttaag aaagaaatca aaaaaagtga tataatactt cttgcgacag
     8161 atggattaac tgggtttatt gatgatgaag atataagaga tttaatatta gattatgaat
     8221 atgagagaac tagtaatata agtgaagaat tgataagtat ggccaatgat gtttctggta
     8281 aagataatgt ttcggttata gtgataaaag tataattagg acggtgatat tgtgggagat
     8341 acaattttag gaaatcgata tgaaatcatc aggaagattg gtgatggagg aatggcattt
     8401 gtatatgagg ctaaagacag attattaaac aggactgttg cacttaaagt acttagacct
     8461 gaatttgtag atgatgatga atttctaaca aagtttaaaa gagaggcaga agcagtagca
     8521 agtctttcac atccaaatat agtaaatgtt tatgatgttg gtgaagatgg aaaagttcac
     8581 tacattgtaa tggagtttgt agatgggaag aatctaaaag agattataca agatgaaggt
     8641 atattagatg aatatactgc attagatata acaaaacaaa tagctatggc acttagtgca
     8701 gcgcacaaaa aggggattat acatagagat ataaaacctc ataatattct catatcaaat
     8761 gaaggaagag tagtaaaagt ggctgatttt ggtatagcca aagctgtttc aaattcaaca
     8821 atgactaata ttggtagtat aataggttca gtgcattatt tttcaccaga gcaagctaaa
     8881 ggtaaatttg taactaataa tgctgacctg tattctctag gtatagtttt atatgaaatg
     8941 ttgataggaa aagttccatt taggggagat agtccaattt ctatagcact tcaacacatt
     9001 aatgatgata tagactttac atcagaagaa aaagttagga ttcctcaaag tgtaagaact
     9061 acaataaaaa agcttacaga aaaatctagt gcagatagat atcaaactgc tgaagaattg
     9121 atagaagata tagagtatat agaaaaaaat attgatttag attttataaa agaatatgat
     9181 gattttgcaa ctaagaaaat tgatgaaaaa gaaattaata aagttgtaaa tccaacactt
     9241 gcaaaaccag ctcctgaaaa agtggttaag cctgttgaag tagctgattt ggatgatgat
     9301 gaagattact atgatgattt ttatgaagaa gatgatgatg aagatgaaga agaggaagag
     9361 ataatgagag ctaaaaagaa tcagagacct aagagtactc caagtaagag aactaagaaa
     9421 aagaagaaaa aacaggaaag cccaaaatct agaagaagat taaaagtaat tgcggctgtt
     9481 ttgatactga tattgtgtgc tcaagtattc ttagcatata agtttttatt tgctggaggg
     9541 tttggcaata aatctttgac tgttcctaat cttgtaaata tgacattaga agaagctcaa
     9601 agtgctgttg aaaaagaagg attatacctg agtgtaaaaa gtgaagagta taatagtgaa
     9661 gttgatgaaa attgtataat atcacagaca cctgaaggtg gaagtacaaa tgtcaaaaag
     9721 ggtgatacta taaatgttgt cgtaagtaaa ggttcaagtc aggcatctgt tcctaatgta
     9781 gtgggactta ctttgagtaa tgcaaaacaa cttattgaag aaaataactt aaaagtggga
     9841 actgttaagt atgaatacag ctcaatatat aaagaaggta cagttttaag tcagagtcca
     9901 ggtgctggct catcaagagc tcaggaaggt gatgaggtaa atctatatgt aagcaaaggt
     9961 tcagaaaaat caaatacaca gactccaaca gtaccagata aaaaaccaac aacaccagag
    10021 aataatacac caacagaacc tggttcaaac tcaggaaaca gtggtggaaa ttcaggaggc
    10081 tcaaattcag gaaatagtgg tggaaactca ggcaataatg gaagtaactc aggaggttca
    10141 aactcaggaa atagtggtgg aaactcagga ggctcaaact caggagataa tggaggaagt
    10201 tcaggaggct caaactcagg agacaatgga ggaagttcag gaggttcaaa ctcaggagac
    10261 aatggagata gttcaggagg ctcaaactca ggagataatg gaggaacttc atcaggaact
    10321 ggcgcaaaca ttggtaaaac agaataaata ttaataaaat aagagctgtc cttttataga
    10381 aaatattttc tttaaagggc agtttgtttt gtataaaata ctgaattggt tataatataa
    10441 ggagaaaagt atttatgaag aagaaaacag ttttaattac aggaggagct agagggattg
    10501 gtaaagctat gtctaaagct tttgcaaaag aaggttacaa tgtattagta aattttaata
    10561 agtcagaaaa tgaagctaaa gagttgtaca ctatattaaa tgaaaaaaat ttttctgtaa
    10621 aactttttaa ggctaatatt tcaaatagag aagatgtaga ggatatggta gattattgta
    10681 taaaagaatt tggaggattg gatgtacttg taaacaatgc tggtgtaagt caagataagt
    10741 tgtttacaga tataactgat gaagattggg ataatatgat gaatattaat ttaaaaggaa
    10801 gcttttattg ttcacaagtt gctttaaaat acatgatatc agaaaaaaaa ggaaatataa
    10861 ttaatatatc ttcaatctgg ggtatatcag gtgcgtcttg tgaagtgcat tattctataa
    10921 caaaagcagg tataataggt atgacaaagg cattagctaa agaagtagga ccatcaaata
    10981 ttagagtaaa tagcatagct ccaggtgtca ttaatactga catgttatct ggatataatg
    11041 aggaagatat agacgctctt gtggaagaaa cccctctaat gaggctagga actccagaag
    11101 atatagcaaa ttgtgctatt ttcttggcat cagacaaatc aaattttatc actggtcagg
    11161 taattagtcc aaacggagga tttgttattt aaaaaaaatg tggtattata taaaaatagg
    11221 aaagaataac acgatatact actcataaac agtatgtgtt tatataaatt ttataaaggg
    11281 aggcatttga tgctagaagg aaaaattata aaggggatta gtggatttta ttatgtagat
    11341 acttataatg gtatttatga atgtaaggct agagggatat ttagaaagca gaaaataaca
    11401 cctttggttg gtgatagagt caaaataagc attgttgatg aagatgaaaa aaagggtatc
    11461 ttagaagaaa tcgacagtag agatacagaa cttataagac ctcctattgc aaatgtagat
    11521 aaagcattaa tagtattcgc aataaaaaat cctaaaccaa acttatctct tttagataga
    11581 tttatagttt tggcagaaaa agaaaattta gaaactgtaa taatccttac aaaagcagat
    11641 ttagatgata atgatacatt agaaactgtt aaaaatatat atgaacttag tggttataaa
    11701 gtcattcctg taagcaatat tactaagtta aatatagata aagtaaaaga agaattaaaa
    11761 gaaaatgtag tagtgtttgc aggaccatct ggagttggaa aatcaagctt attaaatgaa
    11821 atagatgaaa attttaaact tcaaacagga gttgtaagtg ataaaataaa aaggggaaaa
    11881 catactactc gtcacgctga acttttaaaa ttagaatttg gtggaatggt tgcagataca
    11941 ccaggtttta gttcccttgc acttgaagat atagaggaag ttgaacttaa agattatttt
    12001 atagaatttg ataaatttaa tgattgtaag tttggttcaa agtgtattca tgaaaatgaa
    12061 cctaattgtg ctataaaaga agctgttaca aatggagaaa tatctaaaga aagatatgat
    12121 agttatatac agctgctaca tgaaataaga caaaataata gtaggaggta ttagaaacat
    12181 gattaaatta gcaccatcaa tattatcagc agattttgca aaattattag aagatgtaag
    12241 aaaagtagaa agtgcaggat gtgaatatct tcatatcgat gtaatggatg gacactttgt
    12301 accaaacatt acattaggac cacttgtagt caaaagttta aagaaagaaa atataaatat
    12361 ggtatttgat gctcatctta tgatagaaaa tccagaccag tatattgaag aatttgtaaa
    12421 agcaggatgt gatataataa ctgttcacca ggaggcttgt gttcatctac atagaactat
    12481 acaaaatata aaatcacatg gaataaaagc tggtgtagta cttaatccag ctacaccagt
    12541 agatacgatt aaacatgtac tatctgattt agatatggta ttattaatgt ctgttaaccc
    12601 tggatttgga ggacaatcat ttattccttg tgttttagat aagattaaag agttaaaagc
    12661 tattatagat agtcaaggat taaacattga tatagaagtt gatggtggta taagccctaa
    12721 aaacgtagcc gaagttgtac aagcaggagc taatgtaata gtagcaggtt ctgcaatatt
    12781 tggaagtgat gacattcaag aaacagttaa tttatttaga aagaatgctt ctcttgaaga
    12841 attagtttaa atttaataat gaaaatctgt attgttttaa atggcgaaat tgaagattac
    12901 gaagttacaa gagatattat aattaaggaa tgttatgact gcataatatg tgcagatgga
    12961 ggggcaaatc acacttataa aatggaaata atgccagatt atatattagg agatttagat
    13021 tcagtagaag aagaaaagat aaacttctat aaaaataaag gtgttaaatt tgaaaaattt
    13081 ccatctaaaa aagatgagac tgatacagag ctttgtcttt ttttagctaa gacacttaaa
    13141 gcaaatcata ttgatttttt tggtgcttta gggggaagga ttgaccacac tcttgcaaat
    13201 ataaagctat tatattattt aaaagaagat ggtatatatt ctaggatact ttctgataaa
    13261 gaagagatgt atatagttga aaatgaggaa atttcattgt atggaaaccc aggggacact
    13321 atttctgtaa tagctataaa tggtgatgct aagggtgtaa cacttactgg cttagaatac
    13381 cctctcgata attattatat gaaatactct gtacctattg gaatatcaaa tgttatgttg
    13441 agtaattctt gtaagattaa agtagagcaa ggctgtgtac ttgtagttag aaatttataa
    13501 aatttgattt tgacaattgg ttttatattg tagagcagat acaatcaagt aaaatatatt
    13561 tatgattgta tctgcttttt agtatataat tataaatata taataaactt tgacataagg
    13621 gggatttttt gtggtagata gtattatttt tgatttagat ggaacattgt gggattcaac
    13681 tgaagaagta tgcaaagtct ggcaggatgt tttaagtgag cataaggaaa tagaattaag
    13741 tgtcacaaaa gatttattta gaagtcttat ggggctttct tttgaggaaa tagcaaatcg
    13801 tttatttcca aatttaagtg aagaagaaag aatgaattta ttgaatgaat gttctttgaa
    13861 agaatgtgac tatttatctg aaaatggagc taaattattt gatgatatag aagatactct
    13921 taaattacta tctaaaaaat ataaattatt tatagtaagt aattgtcaag ctggatatat
    13981 agaatctttt ttaaaagcac ataaactaga acagtatttt atagattttg aatgtcctgg
    14041 taatactggt ttgcataaag gagaaaataa taaacttata attgaaagaa ataaattaat
    14101 aaatccaatc tatgttggtg atacgcaagg tgatgcaaat agtgctaagt ttgtaggtat
    14161 accttttata tatgccaaat atgggtttgg gaatgtagat gtatatgatt attttataga
    14221 ttcatttaaa gatttgttag aacatgatat attaaaataa gatgagttta aaaattatat
    14281 taaaataata aaaaagttat ggggggattt aaaaatggaa ttacaagata ctatttttaa
    14341 aagacaaagt gtaagaaagt ttaaaaatca agatgtatca gatgaagata ttttaaaaat
    14401 gataaaagca gcaggtgcag caccatcagg caagaatatt caaaactggc attttgttgt
    14461 tataaaacgc cgtgatttaa tggaaaagat agcagatgtg ataactaaaa agcaacaaga
    14521 aatacttgta gaaatggata aagtatcagt ggataaggct aatagattta gaaaatttgt
    14581 taaaaatttt acactatttt acttaaaagc tccagtttta gtattagttt ttacaaaggt
    14641 atataatcca tcaggatatt atgaattaga gcttatagat gcacctaagg agactataga
    14701 taaattattt ataagaaatc caggtatgca gagtctaggt gctgcaattg aaaactttac
    14761 actatcagca attgaacttg gatatggttc ttgttggttg acaagtcaaa actatgcagc
    14821 agatgagata gaagctgttt tagaagcaga aactggtttt gaaaagggag agtatttttt
    14881 aggtgcaatg ttagctcttg gagtgccaga ggacaatttg aagagtccat ctaaaaaacc
    14941 agtagaagaa atatgtacat ttataaaata aaagtcaagg acattttaaa atcgaagtaa
    15001 attaaaaaca aaataaatta aaaataaata tttaaaaaat taaaaagtag gttttattga
    15061 tattaatttt atatttcaaa tcattaattt aattaataca ataagaacta cttttttttg
    15121 tatattaatt ttaaatatat ttaaatcttt agaaaatctt tagaaaagta tatgtaatat
    15181 cttaagtttt taattttaat attaagtata taaaacaaaa tgaaaaaata ttaaatttaa
    15241 actagaagga ggtttctaag atggacatta aa
//
LOCUS       DACULQ010000094        15270 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.094, whole genome shotgun sequence.
ACCESSION   DACULQ010000094 DACULQ010000000
VERSION     DACULQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.094"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(31..1128)
                     /locus_tag="JA154_11490"
     CDS             complement(31..1128)
                     /locus_tag="JA154_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAT7996476.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            1444..2709
                     /locus_tag="JA154_11495"
     CDS             1444..2709
                     /locus_tag="JA154_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAT7996477.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSSKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            2724..4010
                     /locus_tag="JA154_11500"
     CDS             2724..4010
                     /locus_tag="JA154_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M16"
                     /protein_id="HAT7996478.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LEYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            4404..5150
                     /locus_tag="JA154_11505"
     CDS             4404..5150
                     /locus_tag="JA154_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAT7996479.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            5180..6115
                     /locus_tag="JA154_11510"
     CDS             5180..6115
                     /locus_tag="JA154_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HAT7996480.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            6601..6948
                     /locus_tag="JA154_11515"
     CDS             6601..6948
                     /locus_tag="JA154_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996481.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            6960..8939
                     /locus_tag="JA154_11520"
     CDS             6960..8939
                     /locus_tag="JA154_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HAT7996482.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            9106..10479
                     /locus_tag="JA154_11525"
     CDS             9106..10479
                     /locus_tag="JA154_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAT7996483.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            10520..11488
                     /locus_tag="JA154_11530"
     CDS             10520..11488
                     /locus_tag="JA154_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAT7996484.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            11507..12862
                     /gene="murD"
                     /locus_tag="JA154_11535"
     CDS             11507..12862
                     /gene="murD"
                     /locus_tag="JA154_11535"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089164.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAT7996485.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASVIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            12914..14044
                     /locus_tag="JA154_11540"
     CDS             12914..14044
                     /locus_tag="JA154_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HAT7996486.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            14059..>15270
                     /locus_tag="JA154_11545"
     CDS             14059..>15270
                     /locus_tag="JA154_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAT7996487.1"
                     /translation="MMKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIE
                     SEIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGG
                     YVSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDK
                     LVFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVN
                     EDIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISL
                     AEITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLG
                     DRELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKE
                     TTPSIEGQAKVI"
BASE COUNT         6005 a         1660 c         2820 g         4785 t
ORIGIN      
        1 taatagtcaa agtgtatacg attaatcatt ttaaacatta aatctaaata atattacatc
       61 tccatcttta actatgtatt ctttaccttc taatttaacc aacccttttt ctttagcagc
      121 agccatagta ccatgctcaa ccaaatcatt aaaggctatt gtttctgctc tgataaatcc
      181 tctttctata tcagaatgta tttttcctcc tgcctgagga gctttacttc caacttttat
      241 agtccatgct ctaacttctt ttggaccagc agttaagaat gaaattaaac caagtaaagc
      301 ataagaagat tttataagct tatcaagccc tgattgttca agacctaatg tttctaaaaa
      361 ctctttcttt tcttcagcag aatcaagttc agaaatttct gcttctattt gtgcacatac
      421 tacaacaact tcggcatctt ctgtttctgc aaatgcttta acttgttgaa catatttatt
      481 attttctcca ttatcagcta aatcatcttc tgatacattt gaagcatata ttacaggctt
      541 agatgtaagt aaatccaatg aatttacgaa tgtttgttca tcttctgtaa attccatagt
      601 tcttacacac ttactttctt ctaaagtaga cattattgat tttaataaat caagttcaga
      661 acctaaagtt ttatcagcct tagcagcttt ttgagtttta ttaattcttc tttcaagaat
      721 ctctatatct gagaaaatta attctaagtt tatagtttct atatcacgaa gtgggtctac
      781 agaaccatct acatgtacaa cattttcatc ctcaaaacat ctaacaacat gaactattgc
      841 atcaacttct cttatatgtg ataagaattt atttcctaaa ccttctcctt tagaagctcc
      901 tctaactaaa cctgctatat cacaaaattc tatagcagta ggaactattt tttcagaatt
      961 atataattct tgtagtttgt ttaatctttc atctggaaca gatacaactc ccacatttgg
     1021 gtctattgta caaaaagggt aatttgcaga ttctgcacct gcttgagtta ttgcgttaaa
     1081 tagtgtgctt ttacctacat ttggtaaacc aactatacct aatttcatat taatcgtcct
     1141 ctctcttatt cttttttata ttctttatat aactttaaac aatcaatcta taggattgca
     1201 aaattgtttt aattatagac atacatattg attatacatc atatatatca agcttgtaaa
     1261 ctagacttta aagcatattg atttaaataa tatttgtatt taatttaaat aaaaatattg
     1321 cttttagaaa ataataaaaa ggatataatt aaatagaagg tttttatatg tgagtatatg
     1381 taattaaaaa ataaataaat ttcaatttta aaattgatgt ttaaataaaa ataggaggta
     1441 catatggtta atactaaaaa aataataaat ttaggtaata atattaatct tactttgata
     1501 aaaacagaaa agtttaaatc gaatcttata agtttatatg ttcagagact attggatgaa
     1561 aaagaaacta caaaaaatgc tctaatacca tcaataatag caagtggaag ctctaaatat
     1621 cctagtgcta gagcaatatc caataaatta gatgatttat acggttcatc tatgggagct
     1681 gatgctgtaa aacgtggaga acgtcaagta ttgagtttta aagtaattaa cataagtgaa
     1741 aagtatttag atgagagtat atttgaagaa gtagtagaat tttttaatga agtaataaat
     1801 aatacgcttg ttgtagatgg tgggtttaaa gaagaatatt taaatattga aaaagagaat
     1861 ttaagagaaa agatacaatc aataataaat gataaaaagg aatatgcgca agataaatgt
     1921 attgaggcta tgtgtaaagg agagagatat agtgtaagtg aatttggtta tgaagatgaa
     1981 atagatagta taacttcaag agagttatat gaacattata aaaacatact taaaacatca
     2041 cctatagata tagttgtaga aggtaatttt gatgaagata aagttgtaga tataataagt
     2101 aaaaatctaa aatttgaaag agaagaaatt ataaatatac caagagcaga ttttataaaa
     2161 aatgtggatg aggtaaaagt tatagatgaa caaatggaaa taactcaagg taagttagtt
     2221 atgggttata gagcaaatgt ggattatgca gacatagata aatattatgc tttagtagtt
     2281 gggagcaatg tcttaggtgg tggtccacac tctaaattat ttgttaatgt aagagaaaaa
     2341 gaaagtttat gttactatat attctcttca atagagaagt ataaaagtat catgtttata
     2401 tcatctggta tagaaactaa agattatgat aaagctgtag aattgataaa aaaacaagta
     2461 gaaagtgtaa aagcaggtga tatatctgat gaagaattag aaaacagtaa acttgcttta
     2521 gttaattcta tgaaatctat aactgataat ataggtggta tgtctgattt tgttttctct
     2581 caatctatgg caaagacaaa ttctgaagta caagatataa tatctagtat agaaaaggtt
     2641 acaaaaaaag atatagttga agctataaaa aatatagagc ttgatactgt atacttcttg
     2701 agaaattaat ggaggagatg tctatggaaa aaatagttaa tgatatattg aaagaagaag
     2761 tttattatga gaaattgcaa aatggattag atgtatattt tatgcctaaa aggggtttta
     2821 tgaaaaagta tgctatttta gctactaatt atggttcaaa tgacttagaa tttgtaccaa
     2881 ttggagaaga taagaaaatt agagtgaatg aaggtattgc acattttcta gagcataaga
     2941 tgtttgagca acctgatgga ggagatgcat ttgataaatt ttcaaaatta ggagtaaatg
     3001 caaatgcatt tactaatttt acaatgacag cctacttatt ttctgctact gaaaattttt
     3061 atgagagttt agaacatcta attgactatg ttcaaacacc ttattttaca gatgaaaatg
     3121 ttgaaaagga aaaaggtata atagcacaag agataaaaat gtacaatgat gaccctgatt
     3181 ggaatgtata ctttaattgc ttgaaggcaa tgtatgtaaa ttatcctgct agaattgata
     3241 tagcaggtac tgttgatagt atatataaga taactaagga agagctatat aaatgttata
     3301 atactttcta taatccagga aatatggctt tatttgttgt tggtgactta gatgtagaaa
     3361 aagttataga tgtaactaaa aagtcaaaca attacaaagt agataaactc tctaagagta
     3421 tagaaagatt ttatccagaa gaaccagaag gtgtaaaaga aaaagaggtt atagaaaaat
     3481 tccctatatc tatgcctatg ttcaacatag gttttaaaga tagtaatgtt ggtttaaaag
     3541 ggaaagaact tttacgtaaa gagattgtaa ctgatatact tgtagggatg ttatttaaaa
     3601 aaggaagtaa gctttatgaa gatttgtata tgcaaggttt aataaatgaa aattttggag
     3661 ctggattttc gtctcaagtg gattatgctt ttagcataat agctggagat tctaaagagc
     3721 ctaaaaaagt aaaagaaata attcttgatt atatagaaaa atcaaaaaaa gaaggattat
     3781 caaaagaaga gtttgagaga actaaaaaga aaaaaatagg gtcatttatt aagtgctttg
     3841 attctataaa ctttataggt aatagtttta tatcttatgt ttttaaggat ataaatctat
     3901 tggagtattt agatgttatt aaagatatta cttttgagga agttgaagaa agattaaaag
     3961 agcattttaa agaagagtat tgtgttattt ctattgtaga gcctaaatag aaacttaatc
     4021 ataataagaa taactaatta agtattagtt tatgttttta aatgtttatt ttggtaaaac
     4081 aaatttataa aatatattta tataaattga ggtaaaaatt taagagctat agataagaat
     4141 tagaagaggt tgatgtatat atgcatcaat ctttttgatt aaaaaatatc tatgtacact
     4201 tgtaacttta gtcatgagag attcagttta aaaaatttta tagtaattag atataatata
     4261 gtgtagggag ttgtagaata aattttaata ttttttgtag aattaacatt aatgtgttat
     4321 cataatttag tataattaca attaattgta attatagcat attgctttgg aaatattttc
     4381 aattaataca aagaggtgac gttatggata gagtagcatt tacactgttt ggcatagaca
     4441 tcatgtggta tggaatactt atggcatgtg gaatgatttt aggaacttta atagcaataa
     4501 aagaagcaaa aagagtggga attaaagaag atgatgtatt aaatatagct ataatagcaa
     4561 ttccagttgg actaatctgt gcaagaatct attatgttgt atttaattgg agttactatg
     4621 ctcaaaatat gtctcaaatt tttaatttta gaggtggtgg acttgctata catggagggt
     4681 taattggtgg aatattagct ggttatatat acactaagat taaaaatatt aacttcttaa
     4741 aaatggctga tacagttata cttggaatgc ctttagcaca agctattgga agatggggaa
     4801 attttattaa tggagaagca catggaggtg ctaccaattt accttgggga ataatggttg
     4861 atggagtaaa ggtacatcca acttttttat atgagtctat atgggatttt ggaatattta
     4921 tagttttatt actatttaga aaaaataaaa aatatgaagg tcaagttata gttacttata
     4981 taactttata ttcaatagga agatttttta tagagggatt aagaacagat agtttaatgt
     5041 taggaccact tagaatggca caagttataa gtctaattgg agttattggt ggcataattg
     5101 cacatgttta tttatcaaag aaaaataaaa ataatattag tgaagaatag tttaaaaagt
     5161 gaatttaagg gaggtaacta tggaatttca tcatgtatca gttcttttaa atgagtgtat
     5221 agagaattta aatataaagc cagatggcgt gtatgttgac tgtactatgg gaggagcagg
     5281 acattctaaa gaaatagtaa aaaaactgtc agataaggga ttatttatag ggtttgacca
     5341 agataaaaat gctataagca cagcaaaaga gagattgagt gaatatgaaa gtagagtgaa
     5401 gtttgttcac agcaattttg aaaatataaa agaggaatta gaaaaaatag gagtttacaa
     5461 aattgatggt gtattagctg atttaggagt ttcatctcac caacttgatg aagctgatag
     5521 aggattttct tatatgcaag atgcaccact tgatatgaga atggatgtta ggtgtgagtt
     5581 ttctgcatat gatgttgtaa atacttatac agaagatgaa ttaactaaga taataaaaga
     5641 ttatggagaa gataactggg ctaagcgtat agctaaattt atagttgaag aaagagcaaa
     5701 caaaccaata gaaactacag gagaattagt agatgtaatt aaaaaagcta tccctaagaa
     5761 agctagaata gatggacctc acccagctaa aagaactttt caggcaatac gaatagaagt
     5821 aaataatgaa cttggagtaa ttacaaagat gataaatgat gcttcttcta taatgaatga
     5881 aggtggaaga atatgtataa taacattcca ttcattagaa gatagaatag tgaaaaatgc
     5941 atttaagcat ttagcatcag attgtatatg tccacaacat ttacctatat gccaatgtga
     6001 taaagaatct gaggtgaaaa taataactag aaaacctata ttacctagtg aggaagaaat
     6061 agaagtaaat ccacgttcaa gaagtgctaa gctaagagta gcagaaaaaa tataagtagt
     6121 tataaggcta aagaataatc ttatatataa aataagatta ttataattaa ttaaaaagta
     6181 ttatatattt tgttgcactg gtaaagcaat taaaaaacaa ttaacactat aagaaatgtt
     6241 ctttgttatt gagatatata atgaaaattt agatataaaa atatagatat agatggtatt
     6301 aagtaatata atggagaact taggaatcag agagtagata gttagttaca tattatattt
     6361 atggaagaaa ttatctaaaa atactagaag tgataaaaat aaatagaaat taatatctaa
     6421 cctatagatt catataaaag tataagtgaa tttataggtt tttgttgtgt atagaaaaaa
     6481 atacaattag attgtgtaat agtgtattaa tttagcgatg aaattttaga ggaagaaata
     6541 tgattaataa caaatatagt atagaggaaa atttaaacta tatactatcg gggtgagaat
     6601 ttgaaaaaaa attataaaat acataatcta aatgaatata aaaataaaaa aaagaataat
     6661 tataaaaaga gaaataagaa aaaaatagga gctaaatgca ttttactgat tgcaactttt
     6721 tcagtaataa tattgaatct atgtggttat tcaaaaatct cggaaatgaa gtacgatatc
     6781 tattatttac aaaaagattt aagaaaaaaa gagatagtat tagaggaact aaaggcaaaa
     6841 gaaactgaaa aaacatcaat aaaagaaatt gagctaaagg ctaaggaatt gctcaatatg
     6901 gactatccaa aagatggaca aataaagtat atagatgtgg ataactaggg ggaatagatt
     6961 tgagaaaagt aaagaggata agtaagaaaa ggctggtact cgttcttatt ctagcatgtg
     7021 cattgttttt ttgccttgtt attaggactg ggtatctaca acttatgaag ggaaattggt
     7081 tgagtacaaa agcactagaa caacaaacaa gagatatacc aatagaaccc aaaagaggaa
     7141 ctatatatga tagaaatatg aaagagttag cagtaagtgt aactaagtat actgtttggt
     7201 gtaaacctgt ggaagtagaa gataaaaaag aagcagccga aaaggtagct gagattttgg
     7261 atgaagatta taaagatatt tacgctttaa taagcaaaaa aaatatggct cttgtaaaag
     7321 taaaaagatg gatagatgat gataaggcaa gtcaaataag agatgctaaa ttaagtggaa
     7381 tatgggtggc agaagataat caaagatatt acccatatgg gaattttgca ccatatgtgt
     7441 taggacatac ttcttcagat gcaacaggta tatctggtgt agaaatgcaa tatgataaaa
     7501 agttaaaagg aaagcctgga aaacttatag taagtactga tgcttcagga agagaaatac
     7561 cacaaggaat ggaaaaatat tatgagcctg tacaaggtaa tggtcttgtg ttgtctatag
     7621 atgaagtaat tcaacactat actgaaaaag cagtacagaa agcttatgag ttaaataatg
     7681 caaaaaaggt tactgcaata gctatgaacc ctaaaacagg tgatatactt gctttagcat
     7741 caaaaccaga ttatgaccct aatgactcaa gaacgccaat atatccatat tatcaagaag
     7801 aacttgaaaa atataatgat aaagataaaa taaaaggcta ttatcaaatg tggagaaatc
     7861 cagcagtaag tgatacatat gaaccaggtt ctacatttaa gcttattaca tcttccagtg
     7921 ctcttgaaga aggagtaatt aaagatggag aaaaattcac atgtacaggt agtgtaactg
     7981 ttggaggaag aaagattaaa tgttggagac attacagacc tcatggaaca caagaattta
     8041 aacaagcagt acaaaactct tgcaacccag tatttgttga gttgggaagc agattaggtg
     8101 ttggaaaaat gtatgattat atagaatcat ttggtcttat ggataagaca ggtatagatt
     8161 taccaggaga agctaaggga atactgtata acgaaaaaaa tgtaggacca gttgaattgg
     8221 ctacaatatc atttggtcaa tctatatcag taactccaat acaacttata acagctatat
     8281 catcaatagc taatggtgga gatttaatgc aaccaagagt ggtaaaatct tatactgaca
     8341 ataaaggaaa tataactgaa acagtaaaac caaaaaaagt aagaagtgtt atatctaaag
     8401 aaacatctaa aaaaatgtta gaaattgcag aatcagttgt aactgaaggt ggaggaaaga
     8461 tagcatatat accaggttat agacttggtg gtaaaacagg tacagctcaa aaagttatag
     8521 atggtaagta tgctccagga aaatatatat gttcatttgt tggaatagca ccttgtgatg
     8581 acccgcaaat agtagttctt gctattgttg acgaaccaac aggagtaagt gcatttggta
     8641 gtactacagc aggaccaata gtgaaagaaa taatgaatga ctctttaaaa tatttgggag
     8701 ttaaaccagt atataaagaa gaagaaaaag ctgagtatga aaagaagcaa gttaaagtgc
     8761 cagatgtaag aaatctaaaa ataggagatg ctgtaaaggc attggaggat gcgaagttaa
     8821 aaccagactt agatgcagat atagaacttc cagaagatac aaaggtaaag gatatattcc
     8881 caaaacctgg agtaaaagtt aatgaagact caagtataac cctatatttt gaaaactaaa
     8941 aagtaacaga taattaacat atacccataa acatcttcaa aaaaacagaa atatagtata
     9001 taattataaa gttaagagtt atcgtaggca atgtatatat aacggtatac attgcctttt
     9061 gttgctaaga gaatatagct aaggttttaa aggaggagtt taataatgga gtgcttaaca
     9121 ataaaagagt tagtacttgc aactaatggg aagttaatat atggagatta taatgactgt
     9181 gtaagtgata tagttataga tagcagggag gcaaatgccc aaaatgcatt tgtagccata
     9241 gttggagaaa atttagatgg gcatacattt atgaagtctg catatgatag tggatgtaaa
     9301 acttttataa aaaatgaaag taatggaata aaattagaaa gttcggatat aaatttaata
     9361 gaggtaaaag atacatcttt ggctctagga gatatttcaa aatactataa agaaaaattt
     9421 gatattccat ttataggtgt tacaggtagt gtgggaaaaa ctactactag agatatgatt
     9481 tactctgcca tatctgcaaa gttaaatata ctaaaaaatg aaaaaaactt aaataatcat
     9541 tttggagttc cattgacttt atttaatcta aataaagaac atgagtgtgc tgtaatagag
     9601 atgggtatgt ctggattcaa tgaaataaaa tatttagtgg atatagtaaa tcctaaaatt
     9661 gcagtcatat cgaatatagg attatcacat gtagaaaaac taggttcaca agagggtatt
     9721 ttgaaagcaa agatggagat tacttcaaac tttgatgaaa caaatacttt aattgtaaat
     9781 ggagatgata agtttttatc tacattaaaa gaaaaagaac atgtatataa gttaaaaaca
     9841 tttggcttta ataaaaacaa tgatgtttat tgtgaaagtt atactatgga agaggatagt
     9901 ttaacattta tatgtgtaat aaatgggaaa aaagaggaaa tatttatacc aacaatagga
     9961 gaacataata tatataatgc aatgtctgct atattagttg gtttgtgtct aaatatttct
    10021 ttagattata taaaaaaagg tttaaaaaat tttaaaggga caaaaatgag attagacata
    10081 ataaaaaatg aaaaattaac aataattaat gattcatata atgctagtcc agattctatg
    10141 gatgctgctt taaagatact tggaagatat aaaggtagaa gggtagcgat acttggagat
    10201 atactagaaa tgggtgaaat ttcagagtat ggtcatagat tagttggaaa atcatctatg
    10261 aataatactg atattataat aactataggt gaaaattcag tgtttattgg agaagaagca
    10321 aaacaattag gattcaattc agccaatata tatcactttg agaatagaga agatgtgttt
    10381 aacaagttaa atgaattagt aaaaacagga gacactattt tagtaaaagg ctcaagaggc
    10441 atgagattag aaaaaatagt cgaatatcta aataaataag cttaaactaa tgagttaatt
    10501 gacaagggag aggagtttta tgatgttagg aataacagag cttacgtaca ccgcattgat
    10561 tgcgttttta atagtaatta taataggacc aatatttata ccaatgctta gaaaatttaa
    10621 atttggtcag acagttagag atgatggacc ccaaacacat ctagctaaaa acggaacacc
    10681 tactatgggt ggaattataa tgattgttgc aattctaata actggactta caagagtaaa
    10741 agttagtcat gatatggcag taggtcttat atgtattgca ggttttggtt ttattggctt
    10801 tttagatgat tttataaaaa taaagctaaa aagatcactt ggactaaaag catatcaaaa
    10861 aataatcctt caagttgcat tatcttttta tgttgctttt tatcagtata catcatcatc
    10921 aagtgcttca caacttatga taccatttac agattttgtg ataaatgttg gaatattata
    10981 tataccaata atgatgttta taatcgtagc aatagtaaat gctgtaaact tgactgatgg
    11041 attagatgga ttagcatcag gagtaacatt aatagtttct gtattcttta tgctatttgc
    11101 aagttctatt gctggaaata cagaagtcgc agttttagca gcagcaactg ttggagcatg
    11161 tttaggtttc ttaggattta attcatatcc ggctagagtg tttatgggcg acacaggttc
    11221 aatggcatta ggaggtgcag ttgtagcatt ttcagttcta actaactcag tgcttataat
    11281 acctataatt ggaggaattt attttgcgga ggctctatca gttttgattc aagttggata
    11341 ttttaaggca acaagaaaga gattctttaa gatggctcca atacaccacc actttgaaca
    11401 gtgtggttgg ccagaaacaa gagttgtatt tattttttgg attataacag ttgttttagc
    11461 ttggataagt ataatagcag tattttaggg ctagggggaa tgaaaaatgg acttaaaagg
    11521 aaaaaaagtt ttattagtag gacttgcaaa aactggtata tctacaatta aacatttaga
    11581 taaattgggt gcaagtgtaa tcgtaaatga tataaaagat gaaaataaat tgagaaatat
    11641 acttgatgaa ttgaaatcta taaatgacat aaaatacata ttaggacatc acccagaaga
    11701 tgtagatgat atagatatgg ttgtagtgtc tccaggtgta ccactagacc taccattcat
    11761 attgaaatta aaaaattcag gcaagtatat aattggtgag gtagaattag cttttaaact
    11821 atcgaataat ccaattttta tagggattac aggtacaaat ggaaagacaa ctacaactag
    11881 tttggtagga gaaatttttt caagagcaaa acgagataca tatgtagttg gtaatatagg
    11941 aaatcctgta atagatacta tagaaacttc aagtgaagag tctgtgttag taactgaact
    12001 aagcagtttt cagcttgaga gtatagatga atttagacct aaagtaagcg caatattaaa
    12061 tattacagag gaccatttaa atagacacca tactatggaa aaatacatag aagcaaaggc
    12121 aaatatattt atgaaccaaa ctgttgagga tttctgtgta ttaaactatg atgatgagat
    12181 agtaaaatct ttagctgata aatgcaatgc caaagtaatt tacttttcaa gaactaaaaa
    12241 agtaaatgga ggagtatatt tagaaaataa tgacataata atagatatag atgataaaat
    12301 caaatttcta aataaagatg atgtaagttt acctggaggt cataacttag aaaattgtat
    12361 ggcagctata gcaatagcat atgtctgcaa aattgattta gaagttataa aggatgtact
    12421 tatgacattt aaaggagtgg aacacagaca agagtttgta agaaatttag acaatgtaat
    12481 atatgtaaat gattcaaagg gaactaatcc agattccact ataaaagcta tacagtctta
    12541 tgatagacct ataatactta tagcaggggg gatggataag ggaagtaatt ttgatgaact
    12601 tcttgaaact gcaaagtcat atgttaaatc attggtctta ttaggagaaa ctgcatcaaa
    12661 tatagaaaat tgtgctaaaa ataaaggatt taatgacata catatagtaa aagatatgga
    12721 agaagcagtt aagacttctt atgaaatttc aaaaagtgga gatatagtgc ttctttcacc
    12781 agcttgtgca agttgggata tgtatgaaag ttttgaagta agaggaaaag acttcaaaga
    12841 taatgtaaac aatttaaaat aatctcttac ctctaaactt acttaaatgg aagaatggag
    12901 gtagattagt tggatgccta aagaaaattt gaaaaaacaa attgacataa ggatgaagac
    12961 tgaatttgat ggagtagtgt tttacactac tatgctttta gtatttgtag gaatagttat
    13021 ggtgtttagt gcaagtttta tacaatcttc atttaaacac aatgatgcgt actattttct
    13081 aaaaaagaat gtaatatatg ctgtacttgg atttatagtt atgattatta catcaagaat
    13141 agattatagt ttttggaaga aaaatgctac agcaattgga gctatagcag tagtattatt
    13201 attgttagtt ttgacccctt tagggataga agcaaacgga gcaaaaagat ggttgggaat
    13261 aggagcttta acatttcaac ctgcagaaat cgcaaagttt gctacaataa tattaactgc
    13321 aaaattaatt gaaaaaaatt atgataaaat aaaatctttg acaaaaggtg ttgtaccact
    13381 tctcgtagtt ccagggatat tttttgcgtt gataatactt cagccaaatt tgtcaacagc
    13441 aggtactgta attctagtta cttttgtaat gatatttgtt gctggaatgg atatgaaaat
    13501 tgtatttgct atgataggga gtggggctgc tttatttgct gcattagtaa ttgctgaacc
    13561 ctatagactt agtcgtgtta catcattttt agaccctttt caagaccctc ttggtaaggg
    13621 gtatcaggta atacaaggtc tatatgcact aggttcaggt ggattgtttg gtctaggctt
    13681 aggaaagagt aaacaaaagt atttttatat accagagcca caaaatgact ttatatttgc
    13741 tataatagga gaagagttag gattgattgg ttgtataata gttattatgc tatttgtagt
    13801 tttagtctac agatgtgtaa gaatagcttt gaagacttct aatgtatttg cttgtatggt
    13861 tgttataggt ataggtgcac aaattggaat acaagcagct ttaaatatag cagttgcgac
    13921 atcatcaatg ccagctacag gtgtagcact accatttata agttatggag gaacttcact
    13981 caccatcttt atgggagctg taggaatagt gttaaacatc tcaaagcatg tcaaaattaa
    14041 ttaacaggag atgatgattt gatgaaagtt ttattatcag gcggtggaac tggagggcac
    14101 gtgtatccag ctatagctat tgcaaacaaa ataagagatg agcatccaga cgctgagata
    14161 atttttgttg ggacggaaaa gggaatagag tcagaaatag ttcctaagta tggatttgaa
    14221 ttaaagactg taacagttca aggctttaag cgaaaaatag atttcgataa tgtaaaaaga
    14281 gtttttaagc tttttaaagg tttggagcaa tcaagaaaga ttgttaagaa atttaaacca
    14341 gatatagtta taggaactgg tggatatgtt agtggacctg ttttatttaa tgcatctatg
    14401 gggaaaatac cagccataat acatgagcaa aattcttttc ctggtgtaac taataagatt
    14461 ttatctaaaa cagttacaaa ggtacttaca agttttgaag attcgcataa aagatttcca
    14521 gaggcagcag aagataaatt agtttttaca ggaaatccag ttagaaaaga aattttatta
    14581 tcaagaaaaa atattgcaag aaagaatcta tcaataagtg acgaaaaaag aatggtactt
    14641 tgctatggtg gtagtggagg ttcaagaaaa ataaatgatg caatgagact tgtaataaag
    14701 aatatggtaa atgaagatat agcttttata tttgctacag gcaaaagcta ttatgatgaa
    14761 tttatgggaa gtattagcga tataaatctt aaaccatacc aaaaagttgt gccttactta
    14821 gaagatatgg caaatgcact tgcagcaagt gatttggtta taggtagcgc aggagcaatt
    14881 tcattagctg agataacggc tcttggaaag ccatctataa taatacctaa agcttatact
    14941 gctgaaaatc atcaagagta taatgccaag agtatagaaa aacaaggggc aggaattgct
    15001 attttagaaa aaaatctaac tccagagagc ctaaatacag cagtatttaa acttttagga
    15061 gatagagaac ttttggttga tatggcaaat gcaagtaaaa ctataggtaa gcctgaagcc
    15121 atagacctta tatatgatga gataatgaaa gtatacaatt caacacaaaa gtcaacttct
    15181 aaaaaaacaa aaaaagaaaa agttattaag gaagtaaaag aaataaaaaa agaaacgact
    15241 ccaagtatag aaggtcaagc taaggtaata
//
LOCUS       DACULQ010000095        15016 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.095, whole genome shotgun sequence.
ACCESSION   DACULQ010000095 DACULQ010000000
VERSION     DACULQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.095"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..468)
                     /locus_tag="JA154_11550"
     CDS             complement(<1..468)
                     /locus_tag="JA154_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAT7996488.1"
                     /translation="MMFDRVKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYV"
     gene            complement(595..1284)
                     /locus_tag="JA154_11555"
     CDS             complement(595..1284)
                     /locus_tag="JA154_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT7996489.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(1292..1855)
                     /locus_tag="JA154_11560"
     CDS             complement(1292..1855)
                     /locus_tag="JA154_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAT7996490.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(1857..2825)
                     /locus_tag="JA154_11565"
     CDS             complement(1857..2825)
                     /locus_tag="JA154_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAT7996491.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(2926..2969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..3963
                     /locus_tag="JA154_11570"
     CDS             3334..3963
                     /locus_tag="JA154_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7996492.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMYSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            3938..4342
                     /locus_tag="JA154_11575"
     CDS             3938..4342
                     /locus_tag="JA154_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAT7996493.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            4345..4818
                     /locus_tag="JA154_11580"
     CDS             4345..4818
                     /locus_tag="JA154_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAT7996494.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            4994..5257
                     /locus_tag="JA154_11585"
     CDS             4994..5257
                     /locus_tag="JA154_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996495.1"
                     /translation="MSNELKNSLDTLFLYEIKYIFIISKVKNTLVSMFGTFSFYNIIS
                     NLKPISNIDIYLNYRLTKSIHISLKYNLCYSNSYTQPLFTLIL"
     gene            5388..6053
                     /locus_tag="JA154_11590"
     CDS             5388..6053
                     /locus_tag="JA154_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAT7996496.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            6075..7007
                     /locus_tag="JA154_11595"
     CDS             6075..7007
                     /locus_tag="JA154_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAT7996497.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            7028..7669
                     /locus_tag="JA154_11600"
     CDS             7028..7669
                     /locus_tag="JA154_11600"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088178.1"
                     /note="catalyzes the removal of 5-oxoproline from various
                     penultimate amino acid residues except L-proline; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAT7996498.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(7789..8121)
                     /locus_tag="JA154_11605"
     CDS             complement(7789..8121)
                     /locus_tag="JA154_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7996499.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            8387..9007
                     /locus_tag="JA154_11610"
     CDS             8387..9007
                     /locus_tag="JA154_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT7996500.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNKLIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(9048..9233)
                     /locus_tag="JA154_11615"
     CDS             complement(9048..9233)
                     /locus_tag="JA154_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996501.1"
                     /translation="MSRNSVIVISITLEYNIIIYLEIYDVIHGLVINVIIKQRFDIKF
                     NTSVLVLFILHLCLFYL"
     gene            complement(9332..10156)
                     /locus_tag="JA154_11620"
     CDS             complement(9332..10156)
                     /locus_tag="JA154_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HAT7996502.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(10167..11570)
                     /locus_tag="JA154_11625"
     CDS             complement(10167..11570)
                     /locus_tag="JA154_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996503.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(11574..12299)
                     /locus_tag="JA154_11630"
     CDS             complement(11574..12299)
                     /locus_tag="JA154_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996504.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLFAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(12411..13184)
                     /locus_tag="JA154_11635"
     CDS             complement(12411..13184)
                     /locus_tag="JA154_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996505.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(13190..14077)
                     /locus_tag="JA154_11640"
     CDS             complement(13190..14077)
                     /locus_tag="JA154_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996506.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRAGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(14091..>15016)
                     /locus_tag="JA154_11645"
     CDS             complement(14091..>15016)
                     /locus_tag="JA154_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996507.1"
                     /translation="PVLETESYYIDKEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQ
                     TDTGEFSKKIELMSKKKLDNLSVNEYKDISFWKVFNYRVTNSLINVGMLIIISIIISN
                     IYVDEVVTSVKDIILSSKEKKKALNSKIFVALLVPLVIYSVYLMGIFIITCMQQGVPV
                     NGDLESFRIVDNVVALKTNLSITKYVILNIGIELLMFEGWAMIAIFGSFISKSSISSI
                     SFFIFIISITKIMSVIHILPKKLLSVVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNID
                     IVNIAIGLLMAILIGAMSLCFLVIKSDYISR"
BASE COUNT         5398 a         2307 c         1708 g         5603 t
ORIGIN      
        1 aacataatat attaaaaaca taactaatgc aaatctaatt tcccatggat tctttgaata
       61 aacatatttt ttaagtatat catacatatt ttctttatat tttttcatag actttaaatc
      121 agaacaaaat ccatcgcaaa ctgaccaatt atcgatttta ggtataaatc tttttacata
      181 gtataaagtt tcttcaaaat tactatttat attgccaatt accatacctt gtagcctaac
      241 ttcttcatag tatgtatcct ctgctacttc taaaaattcc ttccaattcc cttggcttat
      301 ttcttttgat aactttctta aattaggaac tctaatacct attatatttt ctgttccagg
      361 tataagcttt ttattaaaat ctctatattt tatatcttgt atagacatca tatattctaa
      421 aagttcatta taatccaatt tattccattt taccctatca aacatcattt aattgctcct
      481 taattccttt tttcttattg taacataaaa ctctacaaaa aaagcttcta actattagaa
      541 gctttttcaa aaacataaga aacttttcaa ttttaaattt aaactaaatt aatgctaatc
      601 tcctacacca aaatctgtat tattgtataa aacttctata tttggatttt catccaagaa
      661 tttatactta ctaacaaacc atttcactaa ctcttcatct ctttttatac tagaagaatt
      721 atttataaaa acattcactg tcgctttttc aaaagtattt aggataatct ctatatcttt
      781 atctatcatc tcttttgttt gtccttttat tccaaccata atacaaggtg aatcaaaata
      841 ttcttttaca tcttgtggag tcttaaaatt tgcattttta tttaaaatat tatttctaaa
      901 atcattgtca aaggtttcaa ctccaatttt aaataccaca ggaatttcaa aatactctct
      961 catttcctta agcctatttt tgtaagacca atgactttct aaaaataatt tttttatatt
     1021 tttctctttt ataatacact ttattttttc taaagtatcc ttaggtaatt caaaacagct
     1081 tccagaattt atgacttcaa gaactccata tttcccagtt acgtttttta aaacctttag
     1141 atttaattta ttcatctctt cttctaaatt agaattatca tctatataat cacaaaaaga
     1201 acactttccc catatacaag gaaatccttt aagcagaaca atttctcttt gatttttatt
     1261 tgttatctca ctatatctat ccattttcta tctattttcc tccagtcaat gataccttta
     1321 aagtttttgg aactgcattt aatcctaaat tcactaaaac aactgctaac agcttatctc
     1381 cataatcagt aaatacttgg gttacaaaca cactaaaaac atcccccagt cctaacactt
     1441 tcaagatttg cactatatat gatgaacctg aagaggtcat acctccaaat aaaaatgctg
     1501 aaattattga acttattata gatgttggaa tggcaaataa aagtactcct agtggagttt
     1561 ttaaacccct taaaaaattc tttttaaatg ctattccagc aagtaatccg gttgatattt
     1621 gaactggtgc aaaatagaga gaatacacat caaacgtaat tccacttatt aaacttccaa
     1681 acacacctgt aacaacagcg tatttaggtc ctaaaagtga agctatcatt atagttccta
     1741 ttgaatctaa gtatacaggt aatcttaatc ccatagctat aaaagcgcct attatattta
     1801 tagaaatacc aaaagctata agtgtaatag tttttacaca tatttttctt ttcacactat
     1861 atcactcctt ccacagagtc tataatgtct tcataacctt taaaaagcct ctttaaaaac
     1921 atgtacataa atttcttctc atcaacttca tttagaacaa ttgcattagg atttttttta
     1981 tagaaattga aagaatccac tatagactga cccatagcta ttccatcttc tacaacttct
     2041 acatatgatt caaatccttt acatatactt ctatctataa agtacgctac tgctagaggg
     2101 tcatttatca cacagccaat tattccctcc tgttcccaat ggaaatcaat ataaaatcta
     2161 gttatttcag ttatatactt tgccatcttc ttatcaagtc tatttataaa ctcaataata
     2221 ttaggagtaa gtacaatttt tctagttaca tctaaaccta ccatgtggat ttttttagat
     2281 aaattcttgt aaacataatc tgctccatgt gggtctaccc aataattaaa ctctgctact
     2341 ggagagcaat ttccatgaat cctaaatgct cctcccatag atacaaactc atcgagattt
     2401 tcaaatgctt tcttatcttt aataagtgct ttagctatat ttgtaagtgg tgcaagtgct
     2461 attattgata ctttttcatg attatataaa gtatttatta taaaatccac tgctccattt
     2521 tttgctttag ctcctacaac cttttgataa aagttttctc caataccatc ttctccatgt
     2581 gtatcttgag ctgttacaag ttttcttttt agtggtgctt cttctcctat atatactgga
     2641 atatttagtg aagaacacat ttgaagtgtt tttagtgcat tttctgcacc tatatttgct
     2701 ggaacatttc cacaacatgt ggtaattcca attacttcta gctctggtga gtttaaagcc
     2761 agaagaattg ccaaagaatc atcaattcct gggtcacaat caattattac ttttctcttt
     2821 tccatttttt atcctccttg atttgtggtc gaggagaact acacatcttt tttccacttt
     2881 caaaaaggtg tgtctaaggg tattaattat atactgccac tatataattc cttagttttt
     2941 tatacttgga gttctcgaac aagtacagat gtaaaatcat atttttaggc tacttactat
     3001 agactcttaa aaactataga ctgttaaaaa atttttaaac atctgattta atagctataa
     3061 aataaatttt atagcataaa agaatttatt atattttaat gtatcttatt aaagtttatt
     3121 tagaattatg aaccaacttt aataagatgt ggcgcattta aaccgattat tattatacaa
     3181 taatatctta aatattacaa ttaataacat taatttatta tccataaaaa tatattaact
     3241 tatattattt aataatttat tgacagtatt aaaattaaat tatacaataa cagtgtaaat
     3301 taacttaaca ccgttaagca aagaggtgat tatatgagta tcaaagaaag acgtaaaaat
     3361 gaaaaagaag aaatgaagaa aaagattatg gatgctagca tcgaaattat aaaccaacat
     3421 ggatatgaaa atttatcaat aagaaaaatt gctacaaaaa tagaatattc tccaactaca
     3481 atttatcttt actacaaaga caaagcagag attatcagcg atatgaccaa taaactttat
     3541 aacacagtag aaagtaatgc cattgacatt atgaataatt gctcatcact accaatagat
     3601 aaacaaatca tagaaattat gactagcttt ataaaaactc tttctagtga accagagatg
     3661 gcaaaatcta tcatgtatag tagaatgaat attatatttg catctgaaaa tacaaacaat
     3721 acgcctagca ataatggtat aaaaatgctt gataaattcc tttccattgg aattgaacaa
     3781 ggaatattca aaaaaaatat agataaaagc tcatggatgc ttataagtgc attgttagga
     3841 ttcgttttat gtgttgttga aaataaacta tattctttac ataatttttc tcaacttata
     3901 gaaaatttct tagatatttt agtgggagga ctttacaatg aaaattcgca acaaggctac
     3961 taataaaaag aaaaaatatg taaaaatatc tattgctcta ttctttgtat ttatatgcat
     4021 tattattgta attgtaagca acttaaagcc agaaaacttg gtagtaaaag atatagatat
     4081 taataatgtt aaaaatggca tttatactgg aagtgctgat aataacttgg taaaagctac
     4141 tgtgtctgtt gaagttaata atggtaaaat ccaaaatatt aatatcttaa aacacgacca
     4201 tcttttaggt aaacctgctg aaaaaattac aacaagtatt attaaacaac agtctttaga
     4261 tgtggatgct ataacttctg caacttatag cagtaatact atacgaaaag ctgtagaaaa
     4321 tgctttaaga aagggagaat aattatgaat actattatta tatattcaag taaatatggt
     4381 tgtacaaaag actgtgcaaa tatactaaaa aataaactat cagataatgt cactattgtt
     4441 gatattaata ataataataa aattgagtta tcaaaatttg ataaaattat tattggaagt
     4501 tctatatatg ttggttcagt ttcaaaaaaa atacaagtgc tatgtaatga taatgtagag
     4561 ttattaaaca aaaaacaagt tggcattttc ctttgctgtg gcttttctga acaagcagat
     4621 aaatatttaa aatcaaattt tccatcatcc ttattagaaa gtgcaaatgc tattggaatt
     4681 tttggtagtg aagctcgatt agaaaaaatg aaatttttag ataaacttat tatgaaggct
     4741 gtttcaaaag gtaattatga cagttttaga atatcacagg ataatataga taatttttta
     4801 ataaatctta atagctaaaa taaaactaat tatttctatg ataaattctt ttatttataa
     4861 gtaatattgc tcaaaatagt ctttaaactt tatcaaaatt ttatttttct tagtgaaatc
     4921 taatgaaact agcacttaat atatgcttta ttaatcataa caaatatgtt ttattaatca
     4981 taacaaaaag agagtgtcta atgaactaaa aaattcatta gacactctct ttttgtatga
     5041 aatcaaatat atttttatca tttcaaaagt gaaaaataca cttgtctcta tgtttggcac
     5101 attctctttt tataatataa tatctaattt aaaacctatt tcaaatattg acatatactt
     5161 aaactataga cttacaaaat caattcatat tagcttaaaa tataatcttt gttattcaaa
     5221 ttcctataca caaccattat ttactttgat actttaaaac atcttcccta ttttttatta
     5281 aattttataa taatagtcct attaacgcaa atactataaa aagagtttaa ctttatccat
     5341 taaataaatt taaattaaaa catatagaaa tggaaggaga acaatttatg ataaaactaa
     5401 tcggtatatt gatagttgta attggattta tacttaaaat tgacacatta tttactgtat
     5461 tattagcagg agtggcaaca ggaattgttg caggattaga ttttaatcaa atttttacaa
     5521 ttttaggaga ttcttttgtc tcaaacagag gtgtatcatt attcatatta acattgccag
     5581 taatcggtgt attagaaaga tatggtttaa aacaaagagc tgtttcttta atagaaaaat
     5641 taaaaagact tacaacagga aaagtactta ctatatacat gatagctaga caaatagctg
     5701 gtgctctatc aataagaatg agtggacatc ctcaatttgt aagaccactt gttaatccta
     5761 tggcacaagc tgctggttta agtaatagtg atgaactaaa agaatcggat gaagaagcca
     5821 taaaggcatt atctgctgca tcagaaaact atggaaattt ttatggtcaa aacttatttg
     5881 caggtagtag tggagtatta cttatagcat ctactctgac tcaatttgga tacaatgtaa
     5941 ctggacttaa tatcgtaaag gctagtacta ttatggcagt aatagcactt gtagtggcta
     6001 caatccagtt taccttatat gataaaaaat taaacaaaag tcataaaaaa tagttttatt
     6061 taggaggaat aaaaatgcaa aattttataa acatatcatt agaaatcttt tacgcactaa
     6121 tgggattttt aatgattgtt atagcatata agtcttttac taccataaat aacaataaga
     6181 aatatggtac atcattattt tggatactta tatcactgcc attcatattt ggtagattga
     6241 ttccagccaa tataatagga ataatattaa tattatctag tttattaaca ctttctaaac
     6301 aagttgtatt tgcaaagtat gaagaaccag atgaaaattt tggaaaagaa caagcagaca
     6361 aacttaaaaa taaaatattt ataccatcat taatattagc atttgctgca gtagtagtag
     6421 ctatgagcct ttctaatttt gataattctt ctcaatttgc aataggtgtg ggttcaataa
     6481 ttgcattaat atcagccctt ataataacta aggcaaaacc tgctacatca gttcaagatg
     6541 gctcaagatt acttcaacaa atgggacctg ctagtatgtt accacaactt ttagttgcat
     6601 tgggagcttt atttactcaa gccggagtcg gtgaagttat ttcaaccatg atatctggtg
     6661 tagtccctgc tgatagcaga ttatttggtg ttatagctta tgtattaggt atggttatat
     6721 ttaccatgat aatgggaaat gcatttgccg cattttcagt cataacggca ggaattggta
     6781 ttccttttgt attgtctcaa ggtggaaatc ctgctataat tggagcatta gcattaacag
     6841 caggatattg tggaactcta cttactccaa tggctgctaa ttttaatata gttccagcag
     6901 ccttgctaga atgtaaaaat gattatattg ttataaaata tcaagctcca gttgctttgg
     6961 tattaattat agcccacata ttagtaatgt actttttagg attttaatat aattaggagg
     7021 taaaataatg aaaattttgt taacaggatt tgacccattt ggtggagaac caataaaccc
     7081 agctcaagaa gcagttgaaa gggtcaataa taatataaac ggtgcagaaa tcataaagat
     7141 aactatacca acagttatga caaaatctgt tgaagctata gataaagcta ttcaagagca
     7201 caatccagat atagtaatat cagttggaca agctggaggt agatttgaca ttacacctga
     7261 gagagttgct ataaatatag atgattttag aataaaagat aatgagggta accaggttat
     7321 agatactatt ataaaagaag atggtgaacc tgcatatttt tcaaaattgc ctgtaaaagc
     7381 tatggtaaaa catatgaatg aaaataaaat tccagctagt gtttcaaaca ctgcaggaac
     7441 atttgtttgc aatcatgtga tgtatggaat tctttatatg atagacaaga aatatcctaa
     7501 tataaggggt ggatttatac acatcccata tacaacttca caagtaatag ataaaaaaaa
     7561 tacaccattc atgtctttag aagaaatagt aaaaggctta gaattagcta tagaagcttg
     7621 tattatatat aaagaagatg taaaagaaat tggtggtgaa atttcataat aaatttttta
     7681 gtatttttat aaattaaaaa tactaactat taatttaaat atagactgtg agtagattat
     7741 tcaaacaatt ttactcacag tctttttatg gtatctacag atacaaaatt aaggtctatt
     7801 cctctctccc cactcacaca ttgcatctaa aattggaatc agagattttc ccctttctga
     7861 tagactatat tctaccttag gaggtatttg aggatattca gtacgaataa tcaagttatc
     7921 tgcttctaat tcttttaatg ataaactcaa agttttatag gagatagtat ttatataacg
     7981 ttttaatgca ttatatctta caacttcaaa ttctacaaga cagtaaagaa ttatcatctt
     8041 atatttacca ctaatcagtg aaagtgtata actaaatcct gtttcactta aatctgtatt
     8101 tgatatacag tctatactca tatattacac tctccttttg gttagtattg aacaaaaatg
     8161 tgcgtacttg tttttagaat aatttataat aaaattatag tgtaagaata attgaaagtc
     8221 aattttataa taggaggtgt atttactaaa aaaaaagtta ttttaaatag gattctaaga
     8281 ttagacattt ctatataaat tgataatatt catgaaaatc ctattttata aaaaataata
     8341 tctcttagaa aatctattaa ataaaattca ttggaggaaa tttataatga aagttttatt
     8401 gataaacggt agcccaaacc aatatggatg tacttataca gcactaaatg agattactaa
     8461 agtattgagt aaacacgata ttaaaacaga aatactgtat ttaggtaaag aaacaatccc
     8521 aggatgtatt agttgcgcat cctgttttga aactggaaaa tgtattcgaa atgataaagt
     8581 taataaattg attgaagatc tggataatat tgatggtatt attattggtt ctcctgttta
     8641 cttttctagt gcaactggtc aattaacttc ttttcttgac cgtctatttt tcatagctgg
     8701 aagtcgcatg gctacaaaat taggtgcttc agttgtgtca tgtagacgtg gtggtgcaag
     8761 tgcaacattt gaccaactaa ataaatattt ttcaatcagc aatatgccta ttgtatcatc
     8821 acagtattgg aaccaggtac atggctttac tccagaggat gttatgaagg atgaagaagg
     8881 attacagact atgcgaactc taggagaaaa catggcatgg cttcttaagt gtatatcagc
     8941 aggtaaaaaa gctggaatta aagaaccaca atatgaagaa acaattatga ctaattttat
     9001 aaaataaata aaaaatgtac taatattaat taaaacaaaa cactaattta taaataaaat
     9061 aagcataaat gtaggataaa aagcactaaa acagaggtat tgaattttat atcaaacctc
     9121 tgttttatta ttacattaat aaccaaacca tgtataacat catatatttc taaataaatt
     9181 ataatattat attctaatgt tattgaaatt acaataactg aatttctgct cataaattta
     9241 aatattctac aaatctcatc taaataacaa ttttaataga cctaaaacaa tcctcatact
     9301 gttcattttg tattaaaaat atcacttaca atcaaaaacc atttattaag tatattccta
     9361 agatatacaa tatatataca tggattggat agtataaata aaataaatac ttcatactct
     9421 tccctttttc tccattgtaa agtaacatta aaggtagaga agcaatcatc atccattggt
     9481 aattttgtaa aatagaattt tttaaactat aatctccatt tgacattgtt gtaaatataa
     9541 atatacacat taaaacataa caaatactca aactgaactt tttacctcta cacaaataaa
     9601 aaataatccc aagtataatc caaattggtc caccttctac taaaaatggt actggtacca
     9661 aaatcataaa taaagcaata actctgttag tgactttaaa aagtaaagct actgtaaata
     9721 ttcctaaaat tataggaatg attatcatca ataatcctat aataaaatat ttaatttctt
     9781 tctcttttct aaatttcttt atgaactcta ttgcttgtaa ataaatcgct attaaaaata
     9841 atgttgaaaa aatgttattt ataattatag ctccttctgg atgtgaaaat acattattaa
     9901 ttatttgatt tcctatagcc ataataacag accaaatata caatctactt atatattttt
     9961 ttctgttact agtgtgataa aatccttcaa cagccatgaa tataaacagt ggtgctgata
    10021 ttctgcctac ccaatgaaac caaattggta tttgcattgt ttgtggcata aatgctgcta
    10081 tatggtcaaa tgtcataaat attaatgcta aaattttaat agtaaaccca cttatacctt
    10141 ttttaaaaaa atccatatca tatctcctaa ttttgtttta tctctataat aatttttgtt
    10201 cctattccaa tttcactact tacataaatt ttacctttat gaatctctat tatttcttta
    10261 gaaatagcca taccaagacc tgaaccttta tgcatctcac ctgtattagt acctctataa
    10321 tattttttaa aaatatgttt caaatcactt ttgcttattc caattccatt atcttttatt
    10381 ataatttcta tattatcttt ttttacaatt tctacactta tcaaagttcc ttcgctatta
    10441 tgaactattg aattaaatat aagattggtt atagcccttc tgaataagat tgaatctaca
    10501 tatacttcaa ttaaatcttc attactttca aattcaatat tcctattttt atatctatta
    10561 tcatttaaaa tatcaattat tatatctctg actaaactga ctaaatttat ttttttcttg
    10621 tccagaacta tagtattatt ttttaatcga gtagataaat ttaaatcatc aactaaatct
    10681 tttatatact tagacttatt atatataatt tctgtatact cttgtatttc atcttcttca
    10741 aactcataat ctttgtcatt aattatctct gcataccctt gtatagatgc tagaggtgtc
    10801 tttatatcat gtgttatatt tgccagccat tcctctctca actcttcatt ttcttttctc
    10861 tcaacttcat ttactcttaa agtatcagct aacatattta tatttttaaa aacttcttca
    10921 tatatacctc tgtccttaag atataaacta taatttccat tagccatagt ctcaatactc
    10981 catagtacgc ttgaaattgg ttttgtaagt tttctactaa ataaatatcc tacacctaaa
    11041 gctaccacgc tatcaattac tagtgttata atgataactt tatttaaagt ttttttaatg
    11101 ttattttgag aataggttaa aattattctt gatatatctc tactaggtat acctactatt
    11161 atgtttaaat gttttttgtt taaatatttt tctagtacaa aattaacata ctttgtttct
    11221 atatacttgt aattattcac catttgaaag ggtgtatatt tttttggagt atcctttggc
    11281 acatttactc cataaacttc ctctcctaaa tcatttaaaa cctgtatcca ggaatttgat
    11341 ttttctaatt tttcagctcc aatcttagat agtttaaatt ctccatcttt ttcatataaa
    11401 tctttttcaa aagaacgtgc aaattcttca ggattatttc cagaatctac aactttaaaa
    11461 aagctattag ttgaaatcac atacaatata gatatgatat ttataattac tactgatata
    11521 gctacaaaca caattgtaaa tataaaattt ctagtaatct tccacttcat attctaatct
    11581 tcctttactg ctaatttata tccaagacct tttattgtca gaatatattc aggttttgaa
    11641 ggattatcct ctaatttttc cctaattttt cttatatgaa ccattatagt attgtcaaat
    11701 ccaacataat cttctcccca cactttatcc catagttttt ctttacttat aatttgattt
    11761 tgattatgtg caaataaaga taggattttt agttcctttg caaacagtcc tatagatttt
    11821 ccatctttta taagttctgc cctactttca tttaatatat atgggccaaa tgatataatt
    11881 ttttcttcat tattatttag atttttactt tcgttttgag catcactaaa ataacttaat
    11941 cttctcaaat gcgctttaac tctaaaagcc acttccttag ggctaaaagg ttttgtaaca
    12001 taatcatctc ctccaatcgc tagtccaagt aacttatcta attcttcggt tttagctgat
    12061 aaaaatatta ttggagaatt ggaggttttt cttatttctt tacaaatatc ataaccttcg
    12121 ccatctggta acataatatc caatacaatc aaatcaggat taatactttt aaataattct
    12181 aatccttctt ttttagtttt tgcagtatat atattattaa ggccttcttt ttttaggaca
    12241 gtttctaaaa gcttcaatat ttctacttca tcatctatta ttaaaacacg attttccata
    12301 aacaacctcc tgtaataatt tacatatata ttagcatata ttttatctaa aattcattta
    12361 tcttattttt aattaaacta aaatataaat aaaattacta ttatttttag ttaaaacctt
    12421 ttgcttgatg taaaattttc atatgttatt ttagttaaca ctatcattaa tataattgag
    12481 attactatag aaaaaactat caaaatactt ggtacattat acttaaccat ttgttggctc
    12541 atcataatcc aatttggagt cactgacata aattttatag ctttatctgg tataaaacct
    12601 acactaaagt atggaattgc cattatcaat ccacctagta taaatggaat tacagccgat
    12661 ttaaatatag atgaaagtgt taagcctatt tgtgcaaata taaaagaagc aaaaatagta
    12721 gtcaatataa caattatata catttgtatt actgtaagat ttaaattact atttccaaag
    12781 taccataagt tatgaatcga ttcatttaaa tttttaaaat caaaaaaact aaaacctcta
    12841 attcctacta acaatataaa agaaatatta ataatagata aaattaatcc attagctact
    12901 agttttgata atactactga tttttttccc tttttagttg tatttattaa acctgtcata
    12961 ttagaatcat attctttaat gtaatttcca gaagttatta taaacattat caaacctatt
    13021 agaggtattg agccttctat cttgtaaaat atatctgcat aagtatctaa aacatcaaac
    13081 tttctaatca gaaaaaatcc aaaaaatatt aaaaacagcc aaattatcca aacactcttt
    13141 gattttaata tttttatcat ttcagcttta actaaataca tcatttctac tacacctccc
    13201 ttgcatcttc taaatcaaaa taaaacatgt atacatcttc gaattttgct tgaactggtt
    13261 gtatattatc ctcgcttgga atatcctctg aaacaattct aagtactatt gaattaatat
    13321 cactttgaat acttacaacc ttatatttag attgtatatc atcaacttca cattcatttt
    13381 taacttgaac attatatact ttattttcca tgtgacttaa tatgtctttg tgtgttcctt
    13441 tcattaggag tttgccatct ttaatcatta ttgtttcctt tgcaactgat tctatatcgg
    13501 aaattatatg agtagaaagt attattatct tatctctaga catttttgct attaaattcc
    13561 taaatctagc tctttcttgt gggtcaagac ctgctgttgg ctcatcaagg actattatct
    13621 tagggtcgtt tagtaaaact tgtgctatcc cagctctcct cttcatacct cctgaaaatt
    13681 ttcctataga cttatttctc acatcataaa gacctactaa atctaataat tctcctatct
    13741 tactatttaa atattttccc cttaaacctt ttaaagctcc aatatactgt aaaaattctt
    13801 ttgctgtaaa atttttataa actccaaatt cttgtggcaa atatcctaaa atatttctat
    13861 aatcagcatc cattttaaat atatcctcac cattataaag tatctgacct tcatcaggtt
    13921 ttatcaacgt tgttatttgt ttcattaatg tagattttcc tgctccattt ggtcctagaa
    13981 gaccataaac tccattttct agagttactg taatcttgtc gtttgctact tttttaccat
    14041 atatctttga aagattttct atttctaatt tattcatctt aactcccctt ttatctactt
    14101 atataatctg attttattac taaaaaacat aatgacatag cacctatcaa tattgccatc
    14161 aaaagtccta ttgctatatt gacaatgtct atattcactt taaatatcat aatatcacct
    14221 agataatttc ctattatttt attaaaacca aatatcaaat cataataatt tacatttgat
    14281 acaactgaaa gtaatttttt aggtaatata tggattaccg acattatctt agttatagat
    14341 ataataaata taaaaaaact tattgaagat attgaagact tacttataaa agacccaaat
    14401 atggctatca tggcccatcc ttcaaacatc aaaagctcta tgcctatatt taatattaca
    14461 tactttgtta tacttaaatt agttttcaat gcaactacat tgtctactat tctaaaagat
    14521 tctaagtctc cattaacagg tactccttgt tgcatacaag ttataataaa tattcccatt
    14581 aaataaacag aataaattac taatggaact aataaagcaa caaatatttt agaatttaat
    14641 gcttttttct tctcttttga tgatagtatg atatctttta ccgaagtaac gacttcatct
    14701 acgtaaatat tagatataat tatagatata atgattaaca ttcctacatt tataagtgaa
    14761 tttgtaactc tatagttaaa tactttccaa aaactaatat ccttatattc attcacactt
    14821 agattatcaa gttttttctt tgacattaat tcaatttttt tagaaaattc acctgtatct
    14881 gtttgatttg aaaactgctt taaaacatta atcttatttt gaaatttctc attacctatg
    14941 tcgataccac ttctcgtatc ttctatacgt cctttttctt tatctatata ataactttct
    15001 gtttctaaaa caggcg
//
LOCUS       DACULQ010000096        14796 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.096, whole genome shotgun sequence.
ACCESSION   DACULQ010000096 DACULQ010000000
VERSION     DACULQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.096"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            175..570
                     /locus_tag="JA154_11650"
     CDS             175..570
                     /locus_tag="JA154_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996508.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            613..2712
                     /locus_tag="JA154_11655"
     CDS             613..2712
                     /locus_tag="JA154_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAT7996509.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYNGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(2937..8147)
                     /locus_tag="JA154_11660"
     CDS             complement(2937..8147)
                     /locus_tag="JA154_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996510.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKEIM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNALYEEHKDWIISKREDSGSA
                     GAEGTAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILKSLGYIDFKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(8162..10657)
                     /locus_tag="JA154_11665"
     CDS             complement(8162..10657)
                     /locus_tag="JA154_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT7996511.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIER
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(11340..11627)
                     /locus_tag="JA154_11670"
     CDS             complement(11340..11627)
                     /locus_tag="JA154_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996512.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(11647..11922)
                     /locus_tag="JA154_11675"
     CDS             complement(11647..11922)
                     /locus_tag="JA154_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996513.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(12559..12738)
                     /locus_tag="JA154_11680"
     CDS             complement(12559..12738)
                     /locus_tag="JA154_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996514.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGHAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(13023..14498)
                     /locus_tag="JA154_11685"
     CDS             complement(13023..14498)
                     /locus_tag="JA154_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7996515.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYITLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
BASE COUNT         4827 a         2609 c         1676 g         5684 t
ORIGIN      
        1 attatttcaa ctatataaac tacatatgta aaattcatgt aaatttacta atataaatca
       61 aataaaatta ctaaactctc taaactaatt ttgtaaaaca tactctattt ttaacatttg
      121 ttaccttata cataagtaca aacaaaatct aactaaatga aaggataatg taaaatgaat
      181 attttaaaaa tgaaaaaaac agggatattt ttaggaggag tactttttgg tactgtaggt
      241 gttaagatac tttcaagtga ttgtgttaaa aaaacttgca ctcgtgcaac agcaggagca
      301 ttgaaagtta aagattctgt tcttgaaaca actacgaaaa ttcaagaaaa tgtagaagat
      361 attttagctg aagcaaaaga aatcaacgag caagaagact tatctacatc taaggtaaac
      421 gttgtacaag atactcttaa atgcgaacaa gaagttctta aaactgaaac tataaaaact
      481 gtagaagata ctaattctaa tagcattaaa tgtgaagaaa tcaaagataa cgtaattgaa
      541 gaaaaaaata gtacttgtac aacagaatag atttaatgat tattatgatt aataatatca
      601 ggaggaagta aattgaattt ttatataaaa cacgaaataa gaggtcgtat acgctttgac
      661 ttacaacttg gcaaattaaa caacaagcag tccgatactc tcttatatta tttaacttct
      721 ataaatggta ttaccaaagc aaaagtttac gatagaactg gtgatgcagt catttattac
      781 aatggtgaaa gagaatacat aataagagaa atcactaaat tcagttttaa caatagcaaa
      841 atagaaaacc tagttcctga acataccagc agggaattaa acaattatta tagagacaaa
      901 ttagcaaata aactgctagt caaaagtatc tccaaaatat tttttccaaa gtccttaaga
      961 ttttttttaa ttggacttca ttctataaaa tattttaaaa aaggtctctc ttccctgcta
     1021 aataaaaaaa tagaagtttc tgttttagat gccactgcta taggtatttc tatttttaga
     1081 aaagatatca atacggctgg ttcagttatg tttcttcttg gtatcggtga actattagaa
     1141 gaatggacta gaaaaaaatc tatagacgac ttggctcaaa gtatgtcact aaacattgaa
     1201 aaagtatggt taagaaaagg tgacactgaa atcctaattc caatttcaga gataaaggaa
     1261 ggagatttag taagtgtaac tatgggaaat atgattccat tggatggagt tatagtatca
     1321 ggagaagcaa tggtcaatca ggcatcactt acaggtgaat cccttgcagt taacaaaaaa
     1381 gaaggatctt acatatatgc tggaactgta atagagcaag gcaatattgt tatgtgcgtc
     1441 aaggaaaaag ctggtactac tagattccag aaaatagtaa ctatgattga ggaatctgaa
     1501 aaactaaaat cttctgtaga gagtaaattt gaacatctag cagatacatt agttccatac
     1561 agctttttag gaagtatatt aacctatgca attactagaa accctataaa atcactctct
     1621 atattaatgg tagatttctc ttgtgcatta aaactctcta tccctatttc agtactatca
     1681 gccatgagag aatgtaacaa taacaacatt actgtaaaag gtggaaaatt tcttgaaggt
     1741 gttgcatgtg cagatactat tgtatttgat aagacaggca cattaacaaa agctcaacca
     1801 actgtttcag atataattac attccaaaat tataataagg aggatatgtt aagacttgca
     1861 gcttgcttag aagaacactt cccacactcc atagcaaatg cagtagttta tgaagctgaa
     1921 aaacaagggc ttagtcacaa agaaatgcat acagaagtag aatatattat tgctcatggc
     1981 atttcaacaa aagttgatga taaaaaggtt gtaataggta gcaaacattt tgtatttgaa
     2041 gatgagaagt gtactatccc agacggtgag gaggataagt acaataatct atcagatgag
     2101 tattcacacc tatttatggc aatatcaggt aaattatcag cagttatttg cataaatgac
     2161 cctttacgaa aagaagcaaa atatgtaatt tcaaatttaa gagaatgcgg aataaaaaag
     2221 attgtgatga tgactggaga tagcgaaaaa acagctaaat ctatagcaag taaagttggt
     2281 gttgatgaat attattcaga agtcttacca gaagacaaag ccaattttgt taaaagtgaa
     2341 aaactcaaag gtcgcaaagt aattatgatt ggtgatggaa tcaatgattc tcctgctatt
     2401 tcagaatcag atgtcggaat tgcaatgagt gaaggtgctg aaatagcaag agagatttca
     2461 gatattacca tatcagcaga taatttgaat aatttaatcg ttttaaagca gattagtaat
     2521 aagcttatga aaagagttga tttaagttct aagtttatta tcggctttaa tttaggtttg
     2581 attttacttg gtgtaggtgg atttgttaga ccaagtacat ctgccttttt acacaacgca
     2641 tctactgtcg gaataagttt aaacagtatg acaaatttat tggaaaacac caatacttat
     2701 aactaccatt aatctccatt acataaaaca tatgatataa aaaatttata attcatcttt
     2761 ttaaaaaaat atgtcttaaa attagctttt ttctaatcaa aattatataa agcatacttt
     2821 acaaatttta aactcaactt atttctattt tttacaaaaa aggagatgat tcaaattttc
     2881 attttgaaac atcccctttt gtaaatttct ttaaaattta tatattaatt atattttcaa
     2941 ccaataattg tattttcaac caatctttct tcatagcatt ttaaatcatt ttgctttaga
     3001 taattaaatt cagactctac ataaatacca cagcctacca attcaattgt caaattaggt
     3061 atattttcct ttagtatact agtctgctca taaataattc tttccaataa atcaacttgt
     3121 aatatagaaa aatgcttcat aatctcccca tcactatcta tatctttctt gatttcatac
     3181 caactctttg acaaaattcc cttaaaatca atatacccta gactcttcaa tatatctata
     3241 ctatcgctcc atgcatactt actaggttta tcaaagtatg actctagttt cttcttctgc
     3301 tcatttgtaa gtgaatcata agaaagcttc ttattaaaat gtgccttcaa agaaatctta
     3361 gccatagaat atggacttct accagagttt aatcctgcat ttttgaactt ttcaattcct
     3421 ttatcttcta ctgaagacca tattgagttt attgaatctg aatcattgta tttaacatca
     3481 agaccatgtg taatatttat atagttttgt ggaaatacat attgataata gacatctttt
     3541 ttatcatcat atagagagaa gtatccacta tcatataaat gaatatgcac ctttactttt
     3601 aaactatcct ctaaaatatt tcgtctaagg ttcgggatat ttgcatacat ggttacatat
     3661 tttatggcat caaagttttt atttaatgga acttcaaaac taatttcatc tcctgtcaaa
     3721 agctttttca cttcttcttc attacaatca tatagagaat atgtattttc aaatcccaca
     3781 cgtgatttcc catctaaact gcctgttaat gctaccttca aaatatggtt atctgttttt
     3841 tgccattggt aagcatcttg gtcaagaaac tctctaatct gtctattttg ctctttcgcc
     3901 tgatttataa cctcgttttc atcttctgta atccctacat ggcatctatt taagatattt
     3961 actatattgc ttccaaatgg gaatgtccat agttgaaaag ctgctgcctc aacctgacac
     4021 aagtatctaa cctgatttaa ttcaatttga tggataagtt ttttctgata ttcattcatc
     4081 tgattaccac tatctgacat ttgataaatc aattctccca ttgcaagatt atcttttttc
     4141 ttatcaatct tatcagtaat gataatttga gtttccatca tatcctgtac ttttttaaat
     4201 attatagaaa tctcctctga attttccatt tttaagcttc caaactttgt gcttaattca
     4261 gttaaaactt ccttcatctt taatttactt atctcatttt caaaaatatc ttgattttta
     4321 actgtactat cgaatttttc tacaaatggt ataacagatt ctttcaaagt tttatcttta
     4381 aattccacca tttgagtatt caattcatct aatttcttaa tattattttc tttatcttta
     4441 attgcctctt taactacctc tattttactc tcaaaactag attttacatt aagacaatta
     4501 ttaacaccac taatcgcagt atttataaga gaacaagcag aacttccaat actcaatgca
     4561 tttttaattt tagatgtctt ttgctgtctt tcaatagctt gtttactttt ctctagtgca
     4621 gctaacttat ctttttgctg tttattattg taatttgcct ccaacatacc tatttctttt
     4681 ttctggattt ttgtgtaaat ttccttatac tcgctttcac ttaacccctc ttttgctctt
     4741 tgaatatcgt cagctttgtc tgcataatta tcatcatcta tagacttaac tataatctcc
     4801 ttaatttggt caaatttagt tcttctttct tcatcaacac ctgccaaatt ataagtcatt
     4861 ttatctaaag cattttttct atcttcactt agctgttctc caagtacaaa tctcatacca
     4921 tcagaatcca atttaaaatc tgaaaaatta accatcctgt tagtccattt gtattcctct
     4981 tctttagctg aaagattttc cttcaaagat tttgcactgt caaatgcccc tagacaagct
     5041 tgaccaacac cactaaatgc agtcagtaaa ctcaatgcgc tttgtattcc agaaagaatt
     5101 aactgtgttt tcatttgttc ctcaagtttt gcctgtttct tttttagttc ctctatgtct
     5161 tcttgttcat cttctttcat ctttataatt tcttgaatca acaggttcat ttttgcatta
     5221 acagaaagca ttttattctc aagttcattt tttaaaacct ctatttgttg atttccagat
     5281 tcaatttgag ttaggatact ttccttatat gtattgaaat atccttcttt taactcatct
     5341 ttcttccctt gttccaattc tcttaaggtc ttatccagat aatctgtcaa agaacgaata
     5401 tctgtaacac gatttcctcc aatttcactt tcttcaatca tctgcacata aaatgctgtc
     5461 ttctgctcaa gtgcaaataa aatcatactc cattcgctaa aattctgatt ttttcctaca
     5521 aaagattcta ttctatcaga ctgcatcaat ttatttataa ggtattcata gatttccttt
     5581 gcttctttat aaaattcttt ctttgtattc atatcatcca gagaaagtac tgctttattt
     5641 ttcttctcaa aactatagta cttctgaagt atttctctat ttaattgcat ctctattttc
     5701 tcaactgcat gaaaaatttc ataaatatgt gaaagttcta aaatatcagc ttctaaaatt
     5761 cctactgcac acaacagtct aagatttcta taacctttaa ttccagaaaa accaaatctc
     5821 tcgaaaaatt catagaagaa tgtatctatt gacaaagaat actcctgctt taacaattcc
     5881 ttttctttta gtgcatctcc agagtgaccg gctgttccct ctgctccggc tgaacctgaa
     5941 tcttcacgtt tgctaattat ccagtcctta tgttcttcat aaagagcatt taatttctct
     6001 ccatcatctg ctgcttgctt ctgatttaaa gcatgattaa acacatatct atcaaaataa
     6061 tagtagtttc tatcactatt atccattgga ggagaggtac ctatatatct tattgtttct
     6121 tcatatatac ctctaattcc accaagacct cctgcaccac cagctcctcc agagcctcct
     6181 tttcctgcta aaccatgatt tccatctttt ttacttatag cactttttaa taaaggagct
     6241 tttccaagga gataaacctg tccagacttg cttccaagac ctgcatttcc accattacct
     6301 cctgtgcctc cagttccacc ttgttttcct ttttctgctt ttaccacata gtattttgta
     6361 cctttaacat tagcaaattc ttttgactct tttttagaac ctattttagc atttgcccca
     6421 gcttctccag ttatccctgt atctcctttt tttccatctt gtccatttcc accacgtcca
     6481 ccatctacgt aaagtttaag ttctcctctt ttttctattt ctttaaaaga taatatgata
     6541 tcacctgaag tacctgcatt attgccattt cttccattca ttccgtcgta aatagaactt
     6601 tctccaacca atttttctgc atccttacca gatacgttga ttgaataagt cccttctggt
     6661 atatgtaatt tttctgtaag tagtacaata ttcttacctg agaagacttc tttctccata
     6721 ttacagtcaa ttttcaactc acaatctcca tacatatata tagatgacaa aatattaagt
     6781 tgataaccag atattatttt agtcaaatcc gataaattca ctttaggtat aggaatatat
     6841 atagaaagta cattttcttt ctcatatgat tttatatatg tacaactttg aatcttctct
     6901 atcctatact ctgcatcttc aagctttttg gcttcttctt ccctatattc ttcttctgct
     6961 atccaatatt tgatatcttt taatgtatct tctaaagtat tttttagcaa tgattttaac
     7021 aactcactta gaccaacaat atgagtctca ctacagtaac tatttaactc taaaagaaga
     7081 cgtgtttctt ctaatatatt cactttctgt acgccatcac catatgaaag ttcttgtaca
     7141 aactccataa tctctttcat attattagtc ctactacata aatctgtata catcttatct
     7201 aattgaatct ttttatcttt tagactattt atagaagata gaatggaaat agaatcttcc
     7261 ataccttttt taacactttc tatataagga actaaaagtt tttctaggtc tttataggtt
     7321 acatcaacag ctttttgtat ttctatttta caagaagcag taagcatcaa ccccaaatct
     7381 gttttttcaa atgtttcagg tagatttttc atttcatcca acaacttttc tggctgttta
     7441 tctagtggat tacataattt aatagataca ccatctgata atgctacatt taataagtac
     7501 actaattcct tacaaccgtc gtaatctttc tcttgactat ctaggtcttc ttttgctttt
     7561 tccaatatat ttcttatttt ttcttctgtt acattaccaa ttagtgtttt aggtatttta
     7621 ttgataagaa agaagaaatt ttctttatac tttgcataat caccaagcat atctttcaaa
     7681 tttttgcctg tttctatcaa taatgtatgt ctagagtcca gtagggattg atagtctaaa
     7741 ataactacca tcccttttac ctttgaagat gtggaaatag tttcccttac aagcatagca
     7801 ccagcaatca tatactctgc tccacggtta tcaaaaaaac ctgggcaatc acataatgaa
     7861 aaatctctat ttgcaatatc tacaacttct ggacataaag tcttagaata tacaccattt
     7921 ccaatttcga cagctgaatc ttttgcagtt atatatattt gtccagtctc ttcatccgtt
     7981 ttttcttcca tcacacagcc ttttaaataa tttatacaag tactctttcc aacgcctgaa
     8041 gcacctaaga ataacaaaat tgacttttct tttaagtcat attttctaag ctcgtctctt
     8101 ccttcttcca aaaggtttac gatttcacta taaagtttat ttgccataca tccatccctt
     8161 tctatatttc ggaacaatag ccttgatatt tttctttaat aagattctct tctacctttg
     8221 aaacagctgt tgataactta gtgtttggag cattaaatcc tccattgtct cctctatctc
     8281 catattcagc gtattctctt gctggtcctg ttttaccttc attccagctt ccaccactaa
     8341 attctatatg atatttaatt atactatcta acttataatc ccaacttgta aaatatcctg
     8401 tatagtatac agtcttatct ataccacttg gagaaaatga aagtgtatta tatccgtgat
     8461 agccaccact tcctccatat ccacctaaac ctggtctacc atttggacct gcttctccat
     8521 ctttcactgt tatatcacaa tgattaatta ttctattttt ttgttcctct gaatctgaaa
     8581 tgaagtaaat attacctttc ttaccagcat ttccacctct tccagcattt ccaccagctc
     8641 cacctttttc accttttttg ccacttgtac ctttttcaaa atacccttta aacatctgtc
     8701 cgtggaaata acttgtatat cctgcatcag ctccattact tccagaagct cctgtttcac
     8761 cattttcccc atctattcct ttagaaccat ttcctccatt tgcaatcagt gttagacgat
     8821 aatttctaat ttcttcttta atatataagt aaatattacc tgcatctcta ccatcttcac
     8881 catttttccc atacatagaa ttatctacaa gttctccgct ctttccactt acattaattt
     8941 ttacattact acctacatca agattgttag aagcaagaat aatatttttc tctgatgcaa
     9001 agttcattcc aaggttatca tccatataaa tagtatcttt agcaaagaac ataaatgtag
     9061 aaacattgtc atctactttt ctatttttga aatattccaa tacactgcta attactaagt
     9121 ttggaattaa tgctcttatt tcaatacctt tcttatcatt aatattaatt gtttttacta
     9181 taaatggatt taaaatgtat ttaccaataa attctctaat tatctcatta ccttcttctc
     9241 catatgcact taatcttgtt atactgtctt ctaactcttc attttgtgta actgtaacat
     9301 ttgtcaaaat tgaattaaaa tcacttacac tatatacata atccttttta tttgcaactt
     9361 ccattaattt ttctctattt ggtaatgtaa atacctgttt ttgcacttca taagaaacta
     9421 aaactttttc taaattcttt cttattataa tgaagttttg ttccattatc ttcttacttg
     9481 ccttttctat aataaattcc atatgtacag aaggtaataa aatttcccca gttccatgtt
     9541 ctgtatattt acttaaagca tcaattatat ctaagtttcc ttttatctta ttttgtaatt
     9601 tcatgcaact atcttgtaag aataatccac tttgtttggc taatacgttg ataaatctct
     9661 ctatagtaag acttgttgta ctttccaacc agtttttaaa tacttttaca acctcatcat
     9721 attttgctat ttcttgaatc attgttatac ttccaataat attgataaaa tcttctgtat
     9781 acgccttaac ataatcttct aggctagata cgatattgtt ttgaatttct tttatcaacg
     9841 tatctaaaac taatcttgca tctgcactaa ttggatattg gaatgctgat gtcttatctt
     9901 taacttcatc taattctagt acttgcttta ataagccttc cctctcatct gatgcaagtg
     9961 gcttacaaag atgaatattt tttccattat cttttagcaa ttcttcaaat aaccaagctt
    10021 tttcctctat attactgtct aaattttcgc ataattgttc atattttctt tttataacat
    10081 tatatatttg tttttctgtt cttcctaaga aactttttga aatgaaaaaa gttatactac
    10141 ccttaaaatt ttctttatga agacacatat ttaactgttg gaacaggtct agtacttttg
    10201 taagtctaga atctaatata tctgcttctg gtatcaatac aattactgca catatagatg
    10261 cagaattttt aaatacttca cctaatgaaa ctgctgcact aatatcatgg gctactcccc
    10321 tattgtcgct aaaacctgca gtatcacata attttgcatc cattccttct attggttttc
    10381 tcatatttac aatgttcggg tataaagttt gagaaacagc tcctgaacca atctctgcca
    10441 cagggtctaa gcaatcaata tatgatatac cagtatcttc atcttctact tccatcatta
    10501 cacatcctgc aagatagtta attgcagtac ttttgccaca tcctgtccca ccaagaatta
    10561 gaattaaatt ttgattttca gccttattta ttgctacttt tccttcattt aataagagtg
    10621 ttatttcttc taaaagttga ttgttatttt ctggcattta ttttccccct tggttatata
    10681 ttttatttaa ataaagttta atcccttaag tagattgcaa agtttttata ctatatttaa
    10741 atttgttttt tattttttat ttttaataaa tttgcatttt cactaaattt gtattttcac
    10801 tcaataatta ttcttctttg tttattaaaa taactgtgtt gtataatata atctttatca
    10861 acacagttat tttatttaat gcgcttattt tattgtattt tatgtaatac agttgttttt
    10921 tatattatat gtccattatt ttcctgtggt tattacatat agatttttaa ttatatttgt
    10981 accattaatt taaacattcc tatactactg cttaagtttt acacctatac cagcttgaat
    11041 attttatttt tatttgctta aatagttcaa ctatactggt ttaaataggt tatctttatt
    11101 tgcttaaata gttcaactat actggtttaa ataggttatc tttatttgct taaatagttc
    11161 aactatactg gtttaaatag gttatcttta tttgcttaaa taagttcaac tatactggtt
    11221 taaataggtt atctttattt gcttaaatta gttcaactat actggtttaa atagttttcc
    11281 tttagtttaa ataattcacc tatacctact tgaatagttc acttttattt gcttaaattc
    11341 tataccaata ctggcttaaa cattatacta ccatgattgt attgtctatc ttcaactaca
    11401 ataaaaacag gttctaaatc tttagtagat gttattttta attctaaaaa aacattatca
    11461 gttggtaata tttttacatc ataattattt aaattattat tcgtgatact attaaataca
    11521 tcaccttgac gttcaaaaaa aacattatca actattacaa aataaacatc ttcttttcta
    11581 ctattttcaa cctctatagt attttgttta gttacattta gtctcatctt ccacctccta
    11641 ttaatattac ttaattatat taatacttgt aattgttgca ttatgttcat cacaaggaag
    11701 aggtttaaaa acaactatct ctgctaaata attctgtgga ggatttgtaa ctgaagtagc
    11761 attacaaata aatttaaaat ttgttcctgc tacaacttga gtagaaacaa taagtggttc
    11821 ataaccaacc ccttctaaca ttcctacagc ttcattaaat acttttaaat cttcttcttc
    11881 gacagctctg aattttgacc atccaccaac aaaaagactc atataaaaac ctcctttata
    11941 tatatattta tttatatata tagtagtacc aattttctca atattcaata ttttctgtgt
    12001 aactggtcaa tgaaaatgca taactggata aaaatactta taaaataact taactttaca
    12061 tataaatagc ttaatttaca tgtaataaga ttattctaca atatgaaata catcttattt
    12121 tgaatatatt ctaaatacaa taatggatat tttcaattat tatacttaga aaatatccat
    12181 tacttaattt gttttattta aaatcacata gagactttat tatttttttt attgcatgaa
    12241 tagctatttc aggtatcata aggtgatata ccccctcctt gaaaccacaa aaaacataac
    12301 aaactttacg aatcttgtct taaaagacca caggtacatt ccttatttgt ggattggagt
    12361 agccataaca agcaaatttt acaattttta tgatgacaaa aatcaataaa atcgcttagt
    12421 atataacacg aaatactact tgttgctatt aagaatagtt agataggaag tatgcattat
    12481 accaataaag gaatcaataa agaaagttaa aacatcccta caaattaaaa atctgttaca
    12541 tttttctata tttaaacgtt atttacttac aattggagaa ataacaggtt ttccttctcg
    12601 gtcaagtaaa ggcgtaaatc cggctgcatg gccataatag tggaatacat agttcacacc
    12661 tgtttcttta tccacccaaa tctccgttgc atttattttt ccttgtgaat atactttttc
    12721 aaatctatct tttcccattt tataatcctc catatttttg attggtctat gccttcttca
    12781 cttaagtaga aaaaatacat aacttatttt tttaattcta atatgagcac gtaaaaatgt
    12841 caaacagctt atttaatttc ataacttcat gttactgttc gtaaccttgt ctgtttactt
    12901 acactgcatt acagcaccat tttatccttt acattattgt attgcacaca gtaaaaatgc
    12961 ttactttatt ggatttttca actaaaaaaa ggattttttc tccacagaac atttactatt
    13021 ttttataata tatctaattt tactctcata tcaacattta atgaagatat aacctttttc
    13081 aaatattgta aagactcctt aaaaatatta gaaaaagatt ctgcaaatct ctcaacatct
    13141 ccttttttta aatacctaag tatctcaaaa catacatcaa aacgtgtttt caaaatttca
    13201 ttatattctt catgaaaaaa tctctctaaa tagattccaa atatcatata ttgttttaaa
    13261 tgtaaaaatg tttcctgtaa tacataaatt tgtgtataat ctactatctt acaaagtaaa
    13321 acaaaatctg gataaacctt acactcttca caattatttt gtaagtacct taaatgagtt
    13381 tctattttat ctacagctat caaatccatt ttatttccta gtaaaagtac tacatcctca
    13441 agagtaatcg taacaaactc catagcatcc aaaagcaaac tcaacctttt agctccttct
    13501 tctgtattta tataatcatc atcatattca gaaaaaagtg ttatatatgt tcccttgcca
    13561 ttaattgttt tagcaattcc caattcattt agctcattat aggcacttct aactgttgga
    13621 agagatacat tatatctatt aattatcgaa gtaattgatg gtagacaatc tcctatttta
    13681 atattgcctt caaaaatttg acaaattaca tctgaaacaa ttaagtcata taaataacat
    13741 ttttccaacg ttacagaatt aaaactactt gtatctatac tttgtggagc acgattttct
    13801 accattttaa gtatggattc tctacaagct ttatacatat atgttaatag ttttttaaat
    13861 tcaattagat tcccgtccaa tccatataag taagtttttc ttacctgctc tatgtattta
    13921 ttacgaaaaa agtctataca acaattatcc aagttatcca agttatcgaa attataggcc
    13981 aattgtgaaa taatgacatc atgctgaata atctcacaaa aatggtgaat caggaaatta
    14041 tttgttttac ttattagttt atttatatat tttagccttt gttctttaaa gactaaataa
    14101 ttagaactat ttttttccat acaatcgata atctcgttta attcttcaaa gtctttagat
    14161 gtaaatcttt taatattgta catagcaata gaaggaaata ttaaactaaa aatcttatat
    14221 atttctgata tttgtaaaat cttctcatat aataagtcag attctattga ttgtttactc
    14281 gtactctgga ttaaaactgg tctagaacca gcttttgtct ctataaagcc cttttctgag
    14341 agcattttta ttacagtttt tatagtattt tttgatacca tatattcttc tgacatgctt
    14401 tttatagaat caagcctatc tccaatagta attttaccag tataaatttg ttgaattata
    14461 tgctcaaaaa taattaaata ttgtggtttt ctcttcatat ttttcctctt cgataaatta
    14521 gatttattgt tttttgtaag gtatatttac ttttatttaa tagggcaaaa tagttgctta
    14581 ttttttcctc ataatctata attaatatag accatttata tatatggtct aagttttata
    14641 atactgtaat gcagtaaaat agcatattgt agctatacta cagcaaatta acataccatt
    14701 gtagtatatt aattggaata attttaagta cttcaaaatt ccagcaaaac atatatgtgt
    14761 ttttgatatt atgtatattc gttaatagaa tagcta
//
LOCUS       DACULQ010000097        14523 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.097, whole genome shotgun sequence.
ACCESSION   DACULQ010000097 DACULQ010000000
VERSION     DACULQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.097"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..606
                     /locus_tag="JA154_11690"
     CDS             <1..606
                     /locus_tag="JA154_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7996516.1"
                     /translation="ISGYLTKKLQDLNVDTIRTDILTSPTVTDVIVWNIEQSGTDTFS
                     ATYEVDQQIKEGEQTTTVKATYTVKVHVDADRDMVIIQNPTLAPAIEKSDYEPKTPEA
                     DASVDADTVNDATAFLETFFKLYPTATAKELAYYVFGNVLEPIGRDYLYSELVNPVLT
                     KDGDNMKVKVAVKFLDNQTKATQVLQYELILHKDSNWKIIM"
     gene            819..1676
                     /locus_tag="JA154_11695"
     CDS             819..1676
                     /locus_tag="JA154_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7996517.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPTWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVTSLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            1748..2335
                     /locus_tag="JA154_11700"
     CDS             1748..2335
                     /locus_tag="JA154_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7996518.1"
                     /translation="MSLPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVN
                     ISKGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTG
                     VITSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKS
                     LYSVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            2439..3284
                     /locus_tag="JA154_11705"
     CDS             2439..3284
                     /locus_tag="JA154_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996519.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            3281..3964
                     /locus_tag="JA154_11710"
     CDS             3281..3964
                     /locus_tag="JA154_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996520.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIICFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVEHM
                     WKSLGGVKL"
     gene            3961..4659
                     /locus_tag="JA154_11715"
     CDS             3961..4659
                     /locus_tag="JA154_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996521.1"
                     /translation="MKKILSSTIQIFKQIKSDPMMFAACFSPFIMGTLIKFGIPFLER
                     ITKFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            4671..5582
                     /locus_tag="JA154_11720"
     CDS             4671..5582
                     /locus_tag="JA154_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HAT7996522.1"
                     /translation="MEKGRLLQNKNMNKAIQVSKITLIINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVVSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVPSGIALTAAVLSIVIKEWMYWFTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALNIFKESINKMVDHS
                     CDNATETRIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            6065..6691
                     /locus_tag="JA154_11725"
     CDS             6065..6691
                     /locus_tag="JA154_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7996523.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            6813..7529
                     /locus_tag="JA154_11730"
     CDS             6813..7529
                     /locus_tag="JA154_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7996524.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            7679..8275
                     /locus_tag="JA154_11735"
     CDS             7679..8275
                     /locus_tag="JA154_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAT7996525.1"
                     /translation="MKILWIIIIMADNLLPLLLARFYPNYKHKEMALSVLGSRQSPVK
                     WIYNVWCIISGVVFCIAPYALYQENDSGWAIAIWMLLAIYGIGCEIISGVCPLNENRQ
                     DEDIITKIHGGTSAIGFMALLVVPLLFAILQFQTTKVVLGAISLVCFTIAFIFFCFFI
                     MGEKEKFANTVLRYGGLWQRLVLISCYIPLFVWSVSNQ"
     gene            8362..8553
                     /locus_tag="JA154_11740"
     CDS             8362..8553
                     /locus_tag="JA154_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HAT7996526.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            8577..8807
                     /locus_tag="JA154_11745"
     CDS             8577..8807
                     /locus_tag="JA154_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996527.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            9074..9493
                     /locus_tag="JA154_11750"
     CDS             9074..9493
                     /locus_tag="JA154_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAT7996528.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            9496..9750
                     /locus_tag="JA154_11755"
     CDS             9496..9750
                     /locus_tag="JA154_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT7996529.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            complement(9787..10019)
                     /locus_tag="JA154_11760"
                     /pseudo
     CDS             complement(9787..10019)
                     /locus_tag="JA154_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843377.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10125..10328
                     /locus_tag="JA154_11765"
     CDS             10125..10328
                     /locus_tag="JA154_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAT7996530.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRKLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            10406..11596
                     /locus_tag="JA154_11770"
     CDS             10406..11596
                     /locus_tag="JA154_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT7996531.1"
                     /translation="MSKMKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPTGKRDDKSLREKEKQIKKDLDDGIDTIGGKMTVCQLYAKKNNQKKNIKRN
                     TEIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFHFKLSDVLEDDTEPKVILTPEQEEKLLSFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHE
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            complement(11853..12653)
                     /locus_tag="JA154_11775"
     CDS             complement(11853..12653)
                     /locus_tag="JA154_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996532.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(12643..12831)
                     /locus_tag="JA154_11780"
     CDS             complement(12643..12831)
                     /locus_tag="JA154_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate/nitrate/taurine transporter
                     ATP-binding protein"
                     /protein_id="HAT7996533.1"
                     /translation="MAEAISISDKVAILSKRPATIKNIYDIELNMDGTKTPLKTRKVP
                     DFQKYFDILWKELDTHEA"
     gene            complement(12848..14446)
                     /locus_tag="JA154_11785"
     CDS             complement(12848..14446)
                     /locus_tag="JA154_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAT7996534.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
BASE COUNT         4894 a         2353 c         2688 g         4588 t
ORIGIN      
        1 atcagcggtt atctaacaaa gaaattgcag gacttgaatg ttgatactat tagaaccgat
       61 atactgacta gccccacagt tacagatgta attgtgtgga atatcgaaca gtcgggaacg
      121 gacacttttt ctgctaccta cgaagtagat cagcagataa aagagggaga acagacaaca
      181 acagtcaagg caacctatac ggtaaaagtc catgtagacg ctgacaggga tatggtaatt
      241 attcagaacc ctacccttgc accagcaatc gaaaaatcgg actatgagcc aaagacacca
      301 gaagcagacg caagtgtaga cgctgatacc gtgaatgacg ctaccgcatt tctggaaaca
      361 ttctttaagc tgtacccgac agccacagca aaagagcttg cctattatgt atttggaaat
      421 gtgcttgaac ctatcggcag ggactacctt tattcagaat tggtaaaccc agtacttaca
      481 aaggacgggg ataatatgaa agtcaaggta gctgtaaaat tccttgataa tcagacaaag
      541 gcgacgcagg tattgcagta tgagcttatt ctacataagg atagtaactg gaaaattatt
      601 atgtaagaca ttttgttggc aggatctatt ttcggatata gtctgccttt tctgtagcaa
      661 atgtactgca ctgacaagct atttcattgt attttaagca attaaacctt tcttgacaat
      721 tttatcttaa atcatatatt tatggatatt gactttctgc ttgcaataag ctataatatg
      781 actataaaat caaatcagtc atataggagg tatgtggtat gtataaagaa gttggtattt
      841 cttcaaataa tgttccgata acactttcta tctggtatac agaaagaagt agtcctacca
      901 ttgttttttt gccgggtact atgtcacacc ctctcatgta tgagaatttt ctatgtggtt
      961 tgagtgaaag aggatttaat ataataggca ttcactatct ttcacacgga aaaagcccta
     1021 aaatcaaaaa aaattataca atggaagatt tgctgcataa tgtatatgac gcaacaactt
     1081 acggaataga aaatttcggt gaaaacatag ctgtcatggg ttccagtcaa ggcgggattt
     1141 tagctgcaat ggcggcaggt agggacacaa ggttaaaagc cgtttttcca cataatatca
     1201 tgctgaccac attaaaagaa actatgagct taacaaaata tcccacatgg tcgcaccgtt
     1261 ttatggggct gatacagtgg ggctttcgtg taggtggaaa attgctgcct aactacaagg
     1321 ttccaggaga cgcttattta gactatgaca gagtatttta tagtgaacag gcaaaacaag
     1381 attgcttaaa tgaccctatg cttttaccct attatccttt aagatttgtc accagcttat
     1441 ttaatgctga cttatcgtgc ctggagaatg gggctataca atgtcctgtt attttaatta
     1501 ctgccaaagg cgacccgctt tttagctttg attataccaa caaagtattt gatttaattc
     1561 atgtaccgca aaaggaatta cttgctttag agcttaatcg tcacatgatt tttaatgagg
     1621 acacggacac tacaataaac gcattggaag ctaccttgca taaatatctt ggatagcata
     1681 ttgattatat aatcagtaaa tgttatactt ttcatgacga tataaatatt ttagtcataa
     1741 ggaggttatg agtttgccac gatttacgga acaagaaaaa gaaatcataa acagtaaact
     1801 tttgatagag ggagaaaagc tgtttgcatt acacggctta aaaaaagtaa cagttgatga
     1861 tttagtagct gctgtaaaca tatcaaaagg ttctttctat gctttttatc ccagtaaaga
     1921 acatctatat gttgaaatca attttcgctt acaaaaagag ttatttacaa gtatagaaac
     1981 aaccattaaa aagaaaaagt ataagaacca tagagattta gcaaaggacg ttatcatgct
     2041 aagtttaacg ggggtgatta cttcgcctat tctttcacag attgatttat ctttaatgga
     2101 ctatctgcaa agaaaaatat cgtcagatat ttttgaaaat catatgtaca gtgatattcg
     2161 tatattggag atattggaag atttaggggt tgtatttttg gttccgcata cggttattat
     2221 aaaatccctg tattctgtct tatcctgttt ggaacaattc aaagaggacg aagaactgaa
     2281 tatgatacaa aatcttttag tgaatggtat tattcagcaa gtagtagcag agtaacaatt
     2341 ttgtttggtt atttgacagg caggtagcca aacaaatttt ttacagttta tatgactata
     2401 tatctataaa cgtcataaag ttaaaggagg aatgaataat gattgatgta aaaaaactgt
     2461 attttagcta tactgacaaa ccgtttgtgg agaatgtaag tttccatgtt gggagaggag
     2521 aaatttttgg ttttttagga ccctctggtg ctggaaaatc aactattcaa aaagttttaa
     2581 ctgggcttaa tacaagatat aaaggcagtg taaaagttgc aggtacagaa ataagggagc
     2641 atacaaatca gttttatgaa aatattgggg tagactttga attttcaacc tgctacgaga
     2701 aattcacagc acgacaaaat ctcgcctatt ttgcttctct atatgaaaag caacctcggt
     2761 ctattgatga attattgcat atggtaggct tggaaaatga cggggacaaa aaagttgctg
     2821 acttttccaa aggtatgcgt tctcgcttga atttcattaa agcgttagtc catgaccctg
     2881 atatattatt ccttgatgaa cctacaagcg gtcttgaccc tacaaatagt cgtttaatga
     2941 aagatattat tcttgcggaa aagaaacgcg gaaaaacgat tatcattact actcacaata
     3001 tgtttgacgc aacagagcta tgtgaccaag tagcctttat cgttgccgga aaagtcagtg
     3061 cattggatag cccgcataat ttgattatgt caaggggagc tgccaaaata caatatacct
     3121 actatgataa aggcgagaaa accggagaat gtctgcttga tagaaccaca gaagataagt
     3181 tgttaaaaag tttaatttcc gaaaaccgtt tgctttctat tcatagtagt gagcctacat
     3241 taaatgatat ttttgttgat attacaggga ggacacttca atgagattgg gacgtttaat
     3301 ttgtggtgat atacatttcc aatggaaata tggtttctat tttatctact ttatattaac
     3361 agttttatat gtgtgcggga ttgccgcttt gccggaacat tggaaaacgg atattgcttc
     3421 tattatgatc tactctgacc cggcagcaat gggattattt tttatgggag cgattgtcct
     3481 cttagaaaaa agtcaaaagg tgttaaatgc tatggtggta tcccctgtaa aaatatcgga
     3541 atatatactt tcaaaaacag ttgcgttgat tgctatttct acggtaatcg cattgatatt
     3601 aggggtagtt tccggcagca atcatttact gggtatcgca gttggtacgg ctttgacctc
     3661 agcaatcttt actatgcttg gaattattgc agctacaaaa atttcaaact tgaaccaatt
     3721 tctcattgtg attatgccga tagaaattat ttgctttgta ccacctattg ttggtctgtt
     3781 tgtgaagtta ccatatctgt tccgcttttt tccattcaca gcttgcatga accttataac
     3841 tggaaaaagt gttttactat cgtttgatat ggtacttgta attgctacgt tgattatcct
     3901 ctacatagtt gctcgacata cagttgagca tatgtggaaa agtttgggag gtgtaaagct
     3961 atgaaaaaga tattatccag caccatacag atatttaagc aaatcaaaag tgaccctatg
     4021 atgtttgcgg cttgcttctc cccttttatt atggggactt taattaagtt tggtattcca
     4081 tttcttgaaa gaataacgaa gttttcttta caagcgtatt atccgatttt tgatttatta
     4141 ctttctatca tggctcctgt attgctctgc tttgcatttg caatgattac attggaggaa
     4201 atcgacgata aagtgtcacg gtacttttcg attacccctc ttggtaagtc ggggtatctc
     4261 tttacaaggt tgggagtacc ctcaattatt tcggcggtca tcgcttttat tgtactgtta
     4321 cttttttcat tagaaaagtt gtccattgga atgacaatat gtttggcgct tctcggctca
     4381 gtacaggcaa ttattgtttc acttatgatt atcacattat caagcaataa attagagggt
     4441 atggcagtta caaaactagc tgcacttaca ttgctcggaa taccagcccc cttttttatt
     4501 gatagttact accagttcgc ggttggcttc cttccatcat tttgggtagc gaaagccatg
     4561 cagaatgaag cggttcttta tttctccata ggattggtgg tagctttagt ctggtactac
     4621 ttccttacaa aacgtctgtt tcggaagctg gcaggataag gaggcatatt atggaaaaag
     4681 gtagacttct gcaaaacaaa aatatgaata aggctattca ggtttccaaa attacactaa
     4741 tcatcaatct gagtttatcg ctacttaaat ttgctgccgg atatatagga aagtcaagtg
     4801 ccatgctgtc ggacgctgta cattcggctt ctgatgtagt aagcacaatc gttgtgatgg
     4861 ttggaattaa aatatcagaa aaacagcctg atagagaaca tccttatggg catgaacgta
     4921 tggaatgtgt ggcttccatt attctctctg ttgcattggc tataacgggt gctggaatag
     4981 gatattcagg tatcaagaaa atcttttcag gacagtacaa tacgctttct gtccctagtg
     5041 gaatcgcact tacggcagca gtcttatcta ttgttataaa agagtggatg tactggttta
     5101 caagaagtgc tgcaaagcat acaaattctg acgcacttat ggctgacgca tggcaccacc
     5161 gctctgatgc actctcgtcg gttggttcct taattggcat tttaggagcg cgtttaggct
     5221 atgctattct cgatcctatt gcaagcgttg tgatttgcgg ttgtatctta aaagctgctt
     5281 tgaacatttt caaagaaagc attaacaaaa tggtagacca tagttgtgac aatgctactg
     5341 aaacaaggat tagggaagtt gtgcttcagc agcaaggggt tgatggtatt gatgaattaa
     5401 aaacccgcat gtttggagcg aaaatgtatg tagatattga aatattagct gatggtaatt
     5461 tagctcttta tgacgctcat agaattgctg agggagtcca tcaaacgatt gaaaacaact
     5521 ttccacaatg taagcattgt atggtacatg ttaatacgaa tgaacttctc tattctgcat
     5581 aggacaggga agatgattta aggctattga aagggaaagg agggaagtag gttatggaac
     5641 aatactggtg gattatcatt gttgtggttt tgattgtcgt tggcttactt aaaattgtaa
     5701 ttatgaagaa gtataaccaa aggagaaatt ctaacaatcg ttcaaaagat agcgaggacg
     5761 acaagatgta ttaaggttta ttgaaatctg ttatgttgta gaatgatgtt tttagatact
     5821 agaaagtgct ttaagggggg ctaacgttaa ggaaagcttt gagtacaatt ctttacaatc
     5881 aacagtcttt tgcatagaga aatgtatgta ggaaatatgg tgcaaggcac aaaacaccag
     5941 caattgagaa atcaacctat gaaccaaagg cacactacat tttatactac ttgacaagta
     6001 aacgaacatt tactttaatg gtttaaagaa gtaaacgttc gtttactaaa gaaggaggtt
     6061 gtaaatgtct gacacaaaag aaaatatatt aaaaattgca ctacacttat ttgcagtgaa
     6121 tggttatgag gctgtttctg ttagtatgat tgcggaagcc ttgggaatga caaaaggagc
     6181 attatataaa cattataaaa ataagcaaga tatttttgac tgtatcgtag aacgtatgta
     6241 tcaaattgat aataagcgag ctaaggatta tcaagtacca gaaagcacaa tagaggtaac
     6301 cccaaatgca tacagaaata caactattgg aagtctaata acatttactc ttgcacaata
     6361 cgatttttgg gtgactgatg aatttgcgtc taattttcgc aaaatgttaa tactagagca
     6421 atatagaaat ttaaaaatga atatgcttta tcaaaaatgt cttgtaaatg gtcctatcag
     6481 ttatatagaa gatatattta atgaaatgat tgaaaaaggt gttcttcaag agtgtgatac
     6541 aaaacagctt gctattgaat attatgcgcc attatttcta ttgataagtg tagcagacgc
     6601 agttgaaaat tgttgggatt taaaaactca attaactaca cacgttaaac aatttgtcaa
     6661 gaaatatatt ataaatgaga aagtaaaata atcctttatt ctgtcctaaa tataaaccta
     6721 actttttaat ttcagcagag aaactcaaaa tgaatattgt tataagtcac aaatattaac
     6781 tattaaaata ttacaataac ggagatgaga aaatggaata tattcgcagt aaaaaatatt
     6841 ttacacccga cttacagaca aaaattatgg ggccgaatcc tataaaatta caagaggaac
     6901 tattacaaaa tcataaaatc ccgtctggta gctatgtctg tgaccttgga agtggacaag
     6961 gtttaacttc tgtatttctt gcaaaagaat atggatttgt tgtttttgca gttgacttat
     7021 ggagtgagcc aaaagaaaat gaagaatttt tcaaaaaaat gggattatct tctgcacaaa
     7081 ttaaatctgt aaaagctgac gccaatgaac ttaaatttga gaaagagttt tttgatgctg
     7141 tagtaagtac agattcatat aattattttg gacgtgaccc tatatattta gatgaaaaat
     7201 tgcttccatt tgttaagaaa ggtgggtttg tttatatcac agttcctggc atgaagaaag
     7261 attgccatga gaaacttcca gaagagttat tgttatcatg gacaccagaa gaacttgatt
     7321 atatacatga tataacatat tggtctaaca taattagtca ttgtaaaaag gcagatttaa
     7381 tttcaatata tgaaatggaa agtaattcgg aagtttggtc ggattggata aagcaaaata
     7441 atgaatatgc aataaatgac cgtaaggcaa tagaagctgg cggtggagat tatctgaatt
     7501 ttattgctat aattctacaa aaaaaataaa ttctttagaa ccaaacacta taatttgcgt
     7561 tctaatatgt ttaccgattg acatcaataa ttttatcttt gagggtaatg atttgaaaat
     7621 agaagaatac aagaagatac gaaattgata aattagaatt tgatgggatg gtgatattat
     7681 gaaaatatta tggatcatta ttattatggc agataatctg ctgccgttac tgcttgctcg
     7741 tttctatcca aactataagc ataaagaaat ggcactatct gtccttggct ctcgtcaaag
     7801 cccggtgaaa tggatatata atgtgtggtg cattatatca ggtgtggtat tttgtatcgc
     7861 tccatatgcg ttgtatcaag agaacgatag cggatgggca atcgctatat ggatgcttct
     7921 tgctatatac ggcatcgggt gtgaaatcat ttccggagtg tgcccgttaa atgaaaatag
     7981 acaagacgaa gatataatta caaaaattca cggcggcaca tcggcaatcg gatttatggc
     8041 cttgcttgtc gttcctttgc tctttgcaat tctgcaattc caaacaacaa aagtagtgtt
     8101 aggcgcaatt tcactggtgt gctttacaat agcattcata ttcttctgct tttttattat
     8161 gggtgagaag gaaaagtttg caaatactgt tttgagatat ggcggtcttt ggcaaaggct
     8221 tgttttaata agttgttata taccgttatt tgtttggtct gtttctaatc aataaaattt
     8281 agtttatagg aataacaaat tccagtttgt aaaagtcaat aaatccaaag ataaatttgt
     8341 atttgaaagg agttttgaag aatgaaaaaa tatgagtatg tagaaattaa ctataatgcg
     8401 aaaggagttg tattcctttg tactgataaa cacaaagaaa ttattgacag ttacgcaaaa
     8461 acggggtatc actacgcagg gtttgtaccc acagaaattg acgcaaaagg ctgtatgcga
     8521 aagatagatt taatttttga aaaagaagat taacttattt tagggagttg gttctaatga
     8581 taggacttaa aagacgagat atgaattata ttatgaaagt tggtgctaaa gctcaaggtg
     8641 tttctgtatc tgaatttagg gctatgctac aagataccat agacaaaact atggaaagta
     8701 cagacccaga cgtgcaggca aattttaaac gatactttgg aaataagcgc ccaacaccag
     8761 aggaatatat ttatacgatt accaaaaaga caaaggttaa ggtttaaatg ttgtaaaggg
     8821 ggcttgccgt atgaaacgct ttatgtacaa ttcagtatat tcaagcccaa tttaacagca
     8881 tactatttac aattaaatac gctataccag tagctcgtac aaaatttata ttttgtgcgg
     8941 gcttttttaa tacccgaaaa gtttttttaa ttattttcat ttttttggaa gcaaatcaca
     9001 ccttgctgta cagatatggt gcggaaagag ggataaacac ttttcaattc atagctggaa
     9061 aggaggtgag attatgaaac cgtctgactt ccaaaaaaca attcagtgtc agtttgaatg
     9121 taagctgaaa aaagttgtca aaggtattat ctggaactac cgtaaagaat taaaacgaag
     9181 aaaaaacaag gaaatatctt ttagcgaatt accagacatt acggtagaaa gtctggcaac
     9241 atgggacgac tacgaaacaa actacacaat ttttaatgtg tgtggcatag atattaaagt
     9301 ccttgatgat gaacttgcag aagctctcaa acagctaccc gaaaaaaaac gtaacaatgt
     9361 attgatgtat tacttcttgg aaatgagtga tacagagatt gcagatatta tgaattatac
     9421 cagaggggct gtgttcaaac acagacaccg tgcactagaa actatgaaag agatactaaa
     9481 ggagaagaaa tagaaatgaa gaaaaagtac aagcacccgt cttattattt gatttcatca
     9541 gctatggatg gggatgaaac tgcgattgag aaattactgg cattctatga cccgtacata
     9601 tcaaagtgtt gtttacgtcc tttatatgat gaatacggca atgtttatat tgttgttgat
     9661 atggaattaa agggacgtat tcgagaagcc ttaatcaaaa tgattttaga ctttgagatt
     9721 gatatagata tagaaaccga agaagaataa gcaaatacca gcagggcgtg attggttaaa
     9781 ttcccttctt tcctgccctg ctcctgcttt tatacatgga agcctacggc tttcgccgat
     9841 tgttctttga caactgaata aagcagacag atacgtttgt gatacagcga gccacgggga
     9901 ctgtacgcca tgacctttcc gacaagaaag cgagcgaccc acgcagttga tccgagagcg
     9961 acttgtggaa tggtcgtttt gccatgacct gctatcacag aataatgata cgtccgcatg
    10021 gtgcggttct gcccacaaga atgggaatag ttgagatact aacggagctt tccaaagccg
    10081 tctgtctgct tatgataaga cacacagaaa ggagttgttg caacatgaac aatactgatg
    10141 tgccgatttg ggaaaaatac acccttagta tagaagaagc gtcccgatat tttcgtatcg
    10201 gggaaaagaa gttgcgtaaa cttgccgagg aaaatctgga ctctggttgg gttatcgtta
    10261 atggaaaccg tattcagata aagcgaaaac agtttgaaaa aattattgat acactggacg
    10321 aaatctgatg tcttagagcc gtagatatgg tataatatta gtagcgtatc acggctcatt
    10381 ccatgacgga aaggagcttt acactatgtc aaaaatgaaa agacaagaca gtaaaggacg
    10441 caatttgcgt cttggagaga gccagagaaa agacggaaga tacgtttata aatataccga
    10501 tatattcggg aagccacagt ttgtatatgc ttggaaactt gtaccgacag acaaaacacc
    10561 tactggaaaa cgtgatgata aatcattgag ggaaaaagaa aagcagataa aaaaagacct
    10621 tgacgacggt atcgacacta tcggcggtaa aatgacagtc tgccagcttt atgcaaagaa
    10681 gaacaaccag aaaaagaaca tcaagagaaa cacggaaata ggacgacagt atcttatgaa
    10741 cgcactagaa aatgacccat taggtatgag ggcgattgat acagtcaaac agtccgacgc
    10801 taaagaatgg gctatcagaa tgaatgacaa aggttattcc tataagacaa tagataacta
    10861 taagcgttcc ttaaaggctt cattctatat ggcgatacaa gacgactgta tcagaaaaaa
    10921 tccttttcat ttcaagctaa gtgatgttct ggaagatgat acagagccta aagttatctt
    10981 gacaccagag caggaagaaa aacttctttc ctttatggaa acggataatg tttacagcaa
    11041 gtatcgtgat gaagttattc ttctgctgga aacgggactt cgtatctctg aattatgcgg
    11101 actgacaaca cacattgata tgcttaatcg tgtgattaac atagaccacc agctattacg
    11161 tgatacagag gtgggctact atatttccac acctaagaca aaaaacggca agcgagagct
    11221 tccattgaca gaaagagcat atcaggcact tgaacgaatt ttaaagaacc gaggaaaagc
    11281 tcaaccgctt gttgtagacg gttatagcaa tttcttgttc ttgaaccgtg agggcttgcc
    11341 aaaggtagca ggaaattatg aaagtatggt gcgtgggctg attaagaagt acaacaagac
    11401 ccatgaggac aagttgccga acatcacgcc ccactcattc agacatacct actgtacgaa
    11461 catggcaaat aagggaatga accctaacac cctgcaatac atcatgggac acgcaaacat
    11521 aaccatgaca ttagggtatt atgctcacgg tactttccag tctgcaaagg cagagttgga
    11581 aaggctggct tcttaatatc gaaaatgtat cttactactt atttactact tttggttgca
    11641 ttttcatgct ggtaaatgcc agcttatgcg aggtatcttc caaaaagaaa atgcccatac
    11701 agcccgaaaa tacgggatat atcggcattt accaacttat gaaaagataa tcagaaatta
    11761 gtaagttttt atatcaaaaa aatgattact gtaaagaaat cattttttat tataatcact
    11821 gatacatcaa atattctaaa taaaattttt atttatctgg aattgtatat tttttctata
    11881 atattaacta cagcgtacat tgcccaagca cagatagcca atacaaatac tcccatcatt
    11941 actaaatcta atttaaatac ttgagaacca tatactatca agtagcctat tccatatctt
    12001 gaaactaaaa attctccaac tattacacct acccatgcca taccaatatt aattttagtt
    12061 aaatttatta aatttcctgt gtttgcaggt aatatgagtt taaataatat ttgagattta
    12121 gtagctccaa aactttttaa catcttaatt ttttcttcat ctatattcat aaaataatta
    12181 taagctgaaa gtatagtaac aacaacagaa atcgttacag ctgttacgac gattccttct
    12241 attccagcac ctacccaaac tataattata ggagctaaag cagtcttagg aagagcattt
    12301 aaaactacca aaaatgggtc taatattttt gataattttt ctgaccacca aagagcaatt
    12361 gctaccaaaa tacctaatac agtacctata acaagaccaa gaacagtttc atatacagat
    12421 attccaatat gtttaaataa ttgaccacta aatgcatatt gaataaataa attgtaaata
    12481 tcacttggtt tgctaaatag aaatgtattt ataacattta agtcagctaa aagttcccag
    12541 attacaataa aaccaattaa aattaatagc tggtagaata atacatttct tttttctctt
    12601 tttacacctt taacatagtt tatatatcct tcagatcttt tattaagcct catgtgtatc
    12661 tagctccttc cataatatgt cgaaatactt ttgaaaatca ggaactttac gtgttttgag
    12721 aggcgttttt gttccatcca tgttaagttc aatatcgtaa atatttttta tggtagctgg
    12781 tcttttagat agtattgcaa ccttatctga tatgcttatt gcttctgcta tagctaaaaa
    12841 agtgcaacta attgcttaaa gaaacagaat tatcaacttc agcacccata taaacaatat
    12901 taagatttcc taaactgcta tcccaaacga tactattaat aatagtagat aataacaatc
    12961 tcttatcatc taaactggca ttatcaactt catcattaaa tctcttcaaa ttactaacaa
    13021 taatatcaat attaatactg tcactactaa tattgatgtt actagattcc aaaatattaa
    13081 tcttatcctt taaaatacta atctcattat ttaggctctc aatctgggaa actatataat
    13141 cagaagcagt actaccagta ttttttgcaa gctctataac taaattttta ataagtcttt
    13201 ccttatctaa aatttgattt ttaagtaaat tagtctcaga tttaaaaata ttaaaaacat
    13261 tatcagtctt aatgttatta ctattgaatg cacttataat agactcttct ttacaattct
    13321 taattttatc tataactaaa ctttcaacta atggaccatt aagattttta cagttgcaac
    13381 tatcagaccc aagtgttttt ttagtaccac aaatataata atactttaaa tccttgtcag
    13441 tttttttgcc tttataggta attctcatat tagaaccaca tttaccacat tttataagac
    13501 cagataaaag agcagttttt ccagttgata tccttggagt tttatcacga tttttatcta
    13561 attgattttg tacttcaagc catatttcag gttctatgac tccatggtgt tttgcaattg
    13621 ctgctattga gttagatgta tttttagcat aggtaaggat tccacatttt ctatctggag
    13681 tacctacaac atccatacca ctatttttaa ggtaatctaa aatttcatca ttagctttta
    13741 catatgctgg atttctaagt attctagata aagaactagg gtcaaatttg ccaccacgag
    13801 gaccccttat tccattatta tagacatatt tatataactt agataaggac ctaagttcta
    13861 aatatttatt gtatattagc tttacaatct ccatttgttc atcatttact tgaagcttat
    13921 acatacttct ttcttttaga ttttcatcta agaatgtgat tttttcactg ttgaatccat
    13981 gaggtgtatt gcctccaagc catctaccac tacgggctaa ttcatacata ttgtctttta
    14041 tacgttctgc tattgtttct ctttctaatt gagcaaatac acttgatata aacatcatag
    14101 ctcttcccat tggagttgaa gtatcaaatt gttctttgat agatataaat gatatgctta
    14161 gttcatttaa tttttctatt aaagtagaga agtcagatac atttctcgat atcctatcta
    14221 atctataaca aactaggtaa ctaaatttct tatctttggc atcctgcatc attttcttaa
    14281 attgtggtct atctatattg ctaccactaa atccctcatc ttcatatatc aaaaaatctt
    14341 ttacaccaat agactttgca taatcaatac ataattgtat ttgattttct atagaatcac
    14401 cttttccagt aaatcttgat tttcttgaat aaattgcagc tttcaaaatt aaaaccccct
    14461 ataagttagg atatatattt gaaactattt atctacaacc ttttaagtaa acttattaga
    14521 atg
//
LOCUS       DACULQ010000098        14390 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.098, whole genome shotgun sequence.
ACCESSION   DACULQ010000098 DACULQ010000000
VERSION     DACULQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.098"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(14..265)
                     /locus_tag="JA154_11790"
     CDS             complement(14..265)
                     /locus_tag="JA154_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996535.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            354..1085
                     /locus_tag="JA154_11795"
     CDS             354..1085
                     /locus_tag="JA154_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAT7996536.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            1144..2055
                     /locus_tag="JA154_11800"
     CDS             1144..2055
                     /locus_tag="JA154_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT7996537.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKFIEAGKFELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            2106..2918
                     /locus_tag="JA154_11805"
     CDS             2106..2918
                     /locus_tag="JA154_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAT7996538.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            3027..3719
                     /locus_tag="JA154_11810"
     CDS             3027..3719
                     /locus_tag="JA154_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAT7996539.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            3785..4747
                     /locus_tag="JA154_11815"
     CDS             3785..4747
                     /locus_tag="JA154_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996540.1"
                     /translation="MRKETRLMRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVV
                     LATTIMISNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLNQDNKKNEE
                     ISNNKNLNNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYT
                     SKEKNSEDIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKD
                     VTNARNKCKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLIN
                     TKSPKYIGKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            4784..5941
                     /locus_tag="JA154_11820"
     CDS             4784..5941
                     /locus_tag="JA154_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7996541.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            6093..6653
                     /locus_tag="JA154_11825"
     CDS             6093..6653
                     /locus_tag="JA154_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT7996542.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            6681..7172
                     /locus_tag="JA154_11830"
     CDS             6681..7172
                     /locus_tag="JA154_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996543.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            7401..7952
                     /locus_tag="JA154_11835"
     CDS             7401..7952
                     /locus_tag="JA154_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT7996544.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            8038..8970
                     /locus_tag="JA154_11840"
     CDS             8038..8970
                     /locus_tag="JA154_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996545.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            9025..9888
                     /locus_tag="JA154_11845"
     CDS             9025..9888
                     /locus_tag="JA154_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996546.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            10135..11997
                     /locus_tag="JA154_11850"
     CDS             10135..11997
                     /locus_tag="JA154_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit EIIBCA"
                     /protein_id="HAT7996547.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            12000..13466
                     /locus_tag="JA154_11855"
     CDS             12000..13466
                     /locus_tag="JA154_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HAT7996548.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQRKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIESNSIDFK"
     gene            13490..14326
                     /locus_tag="JA154_11860"
     CDS             13490..14326
                     /locus_tag="JA154_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HAT7996549.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         5664 a         1407 c         2562 g         4757 t
ORIGIN      
        1 ttatttttga taactatctt tttttattta taaattttgc tcctaaatat tttttgatat
       61 cttcttcagg agaaaagtca tcaataagta tttctttccc agtgtcttca aatactacaa
      121 gaacttcaca gtcaccttgt aagaaaaatt ttctaactgt atcttcttta acttctctaa
      181 cttttattac attctttttt tctttttcaa agtcttttgt gtaataactt cccatcatat
      241 taattatttt tgtatatcta ttcataaaat acctccatat gcgattaaat ttaaaaaata
      301 tgttataata attgtttgta ttaacttaat aattataaca aggagtaata ataatgacta
      361 gtataaaaaa taaagatatt ttaaattgta tagattattc cattaaaaat aagctctttg
      421 acaaactaaa tgatgtatat aagtccttgc cagtaggaaa ttgttctgga tgtggaaatt
      481 gttgtatgga gtcagtaggt ataaatttaa ttgagttttt gaatatattt tgttatcttg
      541 aagatagagt tgatttaaaa aaaagctgta taagtaaaat attagattat tattttgagg
      601 agtatacaag aaagaaccca tgtccattta aagatactaa taatagatgt ttaatatatg
      661 aagttagacc attaaattgt aggatgtttg gacattggaa aaaagaggat tataataaaa
      721 atttagataa tgttattgag aaaaataata actatagaga gttaatgaaa aagcaatatg
      781 gatttgaaat aaatgatgaa gtagtaaatt acaggataga ttattgtgat agatttattc
      841 caaataaaga ttatttaagt aaatctaaga gattaagctt ttttgatgag cttatgatat
      901 tagactcaaa tatttattca agtggaaata tagatataga ctttagggat agaggtatag
      961 ttgaatattt tatagagtca ttactttata gaaatttagc atataatgtg aaaataagga
     1021 tatctaaaga atacaaaata agaaaaagaa ctatagatag aataaaaagg attgttcttg
     1081 tatagtttta tattagtaag taataaattg tatcataaat attgtcaaaa tgaggtgaag
     1141 aaggtgaatc ttacaaaagt aaaaggaaat acctttttta taaaaggtgg cacgaataca
     1201 ggaatatatg tatttgaaga taacacagca cttatgatag acccagggtt atgtggttca
     1261 agacctagga gagtaaataa agtattagat gaaaataata tacaggttag atatattata
     1321 aatactcatg gtcatgatga ccactatgga gcttgtaatc aatttaaaga atattataaa
     1381 gatgtgtgta taatgtcatc tgaatacgat aaattatata tagaaaatcc agaattgttt
     1441 tcaaagtata ttgttggggg aaagagcaat gtatttatgg ataatatctt aaaaaataaa
     1501 atgttggatg atataaaaat agataaattt atagaagctg gaaaatttga attaaacaat
     1561 gaaatgtttg atataattga atttcagggt catacacctg gtagtatagc tgttttgacg
     1621 aaggataatg tgatttttgt tggcgattta ttgataggag atgaaatttt atcaaaatac
     1681 gatttcttgt ttctatatga tatacaagaa caaataaatt cattagaaaa attaaaaaat
     1741 atagattttg agtacttagt tttagggcat ggcaagcaag ttatatcgaa agaagattct
     1801 tataaattaa tagaaaaaca tttgaatgct ttaaaaaaat atttatatca aataagagag
     1861 ttgcttgtag aaccgaaatc tttagaaatt ttattaaaaa acattataaa taacaataat
     1921 ttaagcaata attataaaga gtatcatttt tttaaatctt cgttagtatc tgtcataagt
     1981 tacttagttg atttagaaga aatagggtat atattggaaa atggagaatt gctttactat
     2041 acaaaaacaa aataaattat gataaaataa agaaggtcat aaaaaacaaa taaagggtga
     2101 gcaatatgga atacgaaaaa tggaatgaga tactagcacc ttatgaacat gcagtagagg
     2161 aattaaaaat aaaatttaaa aatataagaa aagaatattt agcaaaaggt gaatattctc
     2221 ccatagagtt tgtcacaggt agaacaaaaa aaatatcttc cataatatct aagttaaaaa
     2281 ggataaatgc aaaagatata gaaactgaaa ttgatgatat tgctggtata agaataatgt
     2341 gccagtttgt tgaggatata tatgctatag ttgatttgat aaaagtaaga aatgatatga
     2401 ctataatagg agaaaaagat tatattacaa attacaaaga tagtggttat agaagctatc
     2461 atgttataat aaaataccct ataaactcca ttgcgggttc aaaggaaatt atatgtgaaa
     2521 tacaaataag aactcttgca atgaatttct gggctacaat agaacattca ttaaaatata
     2581 aatatgacca ttacatacct gagactcttg ctgaaaggct tagaagagca tctgatgctg
     2641 cctttctttt agaccaagaa atgagtgaaa taagagagga cataatgaaa gctcaggcta
     2701 tgtaccaaat gaagtctata gctattagag atactctaaa tagaatacaa gagttatatg
     2761 acttgggtga tacacataaa gctatgcagt accaaagaaa acttgataga actgaaacag
     2821 ataaaaatat aactgaaatt ttggaattaa aacaagaaat agatatatta ttagagcaat
     2881 ataaggatac taaggtagat gataaagtag ttcagtaaaa actacttgta gaaaatttgc
     2941 taaatattaa tggggctgcc tcttaatgga aaataatttc taaaaagaga cagcctattt
     3001 agtgtaatta agaaaggaat gatattatga gtttgtatgc tataggtgat ttacattttt
     3061 caacatcagt caataagcca atgaatatct ttggaagtaa ttgggatggt catgaaaaga
     3121 aaattataga taattggaaa gaagttgtta aggaagaaga tatggtctta gtactaggag
     3181 atacatcttg gggaattaat ttatccgaag ctaaaaaaga tttagatata ataagcaaac
     3241 ttccaggtca gaaaatactt ataaagggaa atcatgatta ctggtggaca accgttacaa
     3301 gtttaaataa gctatatgaa gacatgcgtt ttattcagac aaatttttat gaatataaag
     3361 attatgcaat ttgtggaggt aggggctgga tatgtcctaa tgatgttaaa tttgatgaga
     3421 ctgatgaaaa agtttataaa agagaagaac atagacttag actatcatta gaatctgctc
     3481 gtaaaagtgg tcactcaaaa ataattgtga ttactcatta tcctcctacg aatgataaat
     3541 tggaagaatc tttatttaca aagttatttg aagaatataa tgttgaaaag gtgatttatg
     3601 gtcacttgca tggtaaagaa tcttttaaaa tgggattgaa ggggattcga aatggagtag
     3661 aatatacatt agcatcttgt gactatacag aatttaattt aataaaagtt cacgattaaa
     3721 aaacataaat tggtaaatac tataagatac atgattaaaa ataaatattt gagggatttc
     3781 ggttttgaga aaggagacac gacttatgag gtggatatta gtttttatat ttttatatct
     3841 tattccttta gtagtgatat tcagaaatta caagaatttt aagaggtctt gtatctacag
     3901 tagtatttat gtggtattag ctacaacaat aatgataagt aatatttata tgagtggact
     3961 taataaaata aaagagtcta tgtattatca caattatatt tctgatgaga catatgatga
     4021 aaagtatgta tcaaatttta aggaaaaaga aagtactgat ttaaaccagg ataataaaaa
     4081 gaatgaagaa atatcaaata ataaaaattt aaacaacaaa caagatagaa atatacaaaa
     4141 taaagaaagt aatgaagaag gaagtaatga tataaaagaa aagaataatt ctgatagtaa
     4201 tgtttcagtg aataataaat cagaaaaaca agaagataat acaaaagaaa ataataaata
     4261 tacaagtaaa gaaaaaaata gtgaagatat aaaaaaaata gatatggaaa gtataggaat
     4321 ttttaagaaa gaaatttata atatagaaag agcagcttta gttccaatga gagaatgcat
     4381 accttacact aaggatatag caaaaaatat aactaaatta tctagtataa aaaaagatgt
     4441 tacaaatgcc agaaacaagt gtaaagaagt tgtaaaagaa tatgaaaata tgcagattcc
     4501 agaactttct aaggagaagt atacagaaac attgagtaat gctagagatg acgtaaagaa
     4561 agcatatgag cttagagaaa aatctatgga aagtgctttg aaattaataa atactaaaag
     4621 ccctaaatat atagggaaga taacagaata tcttgattta tcagataagc agatagaaag
     4681 ttttaaaaat agattgaacg atttaaaaga agaaataaat aattcagaaa caaataatat
     4741 taattaataa gatttaagga tataagttaa aagggagatt atgatgaata aacagagtat
     4801 aggaaaacta aatatgtttt ttatggggat tactcaaaga aaccttgata atagggattt
     4861 ttttattgag ttagaggtga tttttaaatc tggaagaaaa gagtttaaag gaattgcaat
     4921 agaggaagat aataaatata aatttaattt taaaggaaaa tcagatttat atacgtttaa
     4981 tgagttgctt aagaatattt ctaaagaggc agaaaattat gatggattag tatttaaata
     5041 tgttgagcga ggaactattg ttgtaattga aggagacaat aagaaggtaa atgtaaaata
     5101 tttagataat aaggaagaag tacctaaaat agatgaattt acggcatctc aaataaaaaa
     5161 cagagattat tatgtaaaag taggacaagc aaatgcactt ttaaaagaaa ttggagtact
     5221 tacaaaagat ggtaagataa aaaatgataa aataagaaaa tacaatcaga tagaccattt
     5281 tgttgaattg atagatagta ttttaaaaga aataaaggat aaagactgta taactatctt
     5341 ggattgtgct tgtggaaaat cctacttaag ctttgtctta aatttttata taaaagaagt
     5401 attgaagaaa aactgttatt ttataggaat agattattca gatgtagtta tagaagcatc
     5461 aaaaaatatg gctaaaaact tgggttataa aaatatgtca tttataaaag aggatttgac
     5521 taattataca cctaataggg atgttgacct tgtaataagt ctacatgctt gtgatacggc
     5581 tacagatatg gcaataggtc taggaattag agcaaaatct gaagctatag tagtagtacc
     5641 atgttgtcat aaggagctat tagggcaata tagatatgaa gctatggaac caatattaaa
     5701 acatggtgta tttaaagcta gatttgcaga tttaataact gatggtttaa gaacattgtt
     5761 attggagggc aatggatatg atacttctgt agtagaatac atatctccac ttgatacacc
     5821 aaaaaattta atgataagag cgataaaaac taaaacgaat aatgacaaag cattaaaaga
     5881 atataaagaa ctgaaatcac aatttggagt ggaaccaacc ttagaaaaac ttatatatta
     5941 gttaaaaatg taaaatttac gaaaagcttg cttaaatatg caagcttttt ggtattatat
     6001 aggtatagtc atatccagag ttttgattta taattaaagc tgtgctataa tatttgatta
     6061 atcaaatcaa taaatgttac atggaggtaa aaatgaacaa attgaatata tgtattgata
     6121 tagatggaac aataacaagc ccttatcatt ttcttccata cctaaatgat atctttaata
     6181 aaaatataac tgaagcagag tgtataacac ataattggga agaattgtat ggttcgggaa
     6241 taagagatgt gtatgcggag tttaataata agtatataca ctcatatgat gaagcaaaga
     6301 ttgtagaggg tgctacagag gtaattagca cattatctaa aggacataat ttatcttttg
     6361 taacggctag acatgagtgt ttaactgatg taactaaaaa ttggctatcg agacaaggat
     6421 tttcagatat agatgtttac ttattaggaa gtgattataa agttgaaaag gcaagagaac
     6481 tgagttgtga catatttata gaagataatc ctttaaactc agtgcaacta gctgatgatg
     6541 gtgttaaagt aatacttctg gatactaact ataataaaga tgttaaacat caaaatatag
     6601 ttagagttag taattggata gatattgaaa agattataaa acaaggtatt taaagatttg
     6661 tagattgaaa gggggagcat atgatagatt taaaaattga aaatagaaaa ggaaaaatcc
     6721 atgtaaagag cagtgaaata aaagacattt tagaattacg tccagacttc gaatatgtac
     6781 aagatatatc aaatactata aatcaagaaa atatattggt atttgattgc cagttgacag
     6841 gaaattcttt tgatatggat gatttagata tagaagaaat attggaagag ttaggtgaag
     6901 aaatagatga gtcttatttc agtgttttat ttgaagatgt aagagcgtat ttaaaagatg
     6961 ctactgatga gatagaagca gacttacagg ataattactt atttgataat ttgagatgtt
     7021 attttgatat atacaatata aatcaagaat ttacagattt taagtttgta tttttagttt
     7081 cttttaaaga tataaaaatt tcttcattag caaacctagc taagattgtt tctaagagac
     7141 agcttatagg tgcatctaag ttttatagtt aaaatgaaag attattataa taaaagctag
     7201 taaccttgta aaaatatagg ttactagttt tttatttatt cattttcaca aaatttcaga
     7261 aaactattta cattctaaaa aaagtaatgt atattaaaca taatgtacat tataatggat
     7321 aaaaagtttc tattaaatta ctaagtttat ttaataatca aaataattta tcttatatta
     7381 gtatgtatta gggggtattt atgcaaagtt taaatgaaat aattgctgaa aatttaaaaa
     7441 agataaggaa agaaaaacat cttagtttag acaagattgc acaacttagt ggtgtaagta
     7501 agagtatgct atctcaaatt gaaaagtgtg aggtaaatcc tactatatct actttgaaga
     7561 aaataactaa tgggctaaaa atctcattta catcattgat ggagaggcag gaaagtgaca
     7621 tagaattgat tcaaaaaagt gatatagacc attttattga agataatgga aagtatataa
     7681 gttatccaat atttccattt gactccaaaa gaagatttga gatatttatg attgaaatag
     7741 aagaaggtgg aaatttagat tcaaatgctg aacttccagg aacacaagag tttataactg
     7801 tttttagtgg cgaagtaacc attaaaataa atggagaaga ttacattgta tctagtggaa
     7861 attctataag atttaaagct gatgtatcac atatatatag aaactcaggc aaaggtatag
     7921 ctaaaatgag tatggtagta tattatatat aaataattta atggaattag agtgatattt
     7981 agatgatttt aggtatgaag atatattata tttaagttat aggagtgtga tttatggatg
     8041 aataataaaa aagcagtttt tttcttttgc agtgatttaa agaaagaccc agtagccagt
     8101 aatgtgataa aatgttgtga agaaataatg gatttaaatc aaactgatat tgttattgat
     8161 gataattttg tattagagtt taaggataaa aacaataact tatttcattt tgtaaaaaca
     8221 aaagatgtaa taagtcataa ttataaatat tatcttccaa ttttaaatag acattttaga
     8281 gattatgatt ttgcaggagt tgtaaattgg catgagggag aaaatgctcc agaacatatc
     8341 ctaacagctc atacaattgg agatgttcca acaggagaat ttggaaattc aaatccaaga
     8401 tactttaaaa atctaatcaa tgctattgag gatataaaaa atgaaaatct acttgatgaa
     8461 tttactacat taacagaagc aactcactgg tctggaacaa catatggtga agaatctaaa
     8521 ttaattacag aatattctgt acctatgttg gatattgaga taggaagtag tagtgatagt
     8581 tttaataata gtatagcaat acaggtattg gcaaaatcat taattagagt atttgattgt
     8641 gatgaacctt tgaagacttt gttatgtgtt ggaggggtac attttgagaa atctttctca
     8701 gatattataa aaaataaaga atataacata tcaattggac atgtattgcc aaatcaatgg
     8761 attgtaagtg gaatgtatga tgatgaatct ggatttgaga aactagaaaa gtgtataaat
     8821 agtattgaag gaggaattga ttgtatagtt tttcatgata aattaaaagg tacctataaa
     8881 gagcaatgtc gtaaactagg tgaaaaactt aatgtacctg tatttaaaca taaaatatta
     8941 aaaaatccaa aagacttacc tatatggtag ttttatacag ttaatataac aataagtcgg
     9001 attaattttt aagcgaggtc aaaaatgata ttatcttatt ttgcattatt aattagagta
     9061 gtgctattag cttttgaaag agtagttgta agattattag gtgatgaaga aggagatatt
     9121 tataaaaata tagcttcatc atttctattt ttctttatag ggggagtatg tttacttcca
     9181 tttagcataa tggatagagt tagtgattgg tcgtttttaa taccatgtta tataagtagt
     9241 ttagtctatt caattggttc agtagcgtat gtaacgtctt tagcaactgg ggaagtttca
     9301 cttgttactc caataaatag tttaaattca ttgtttttac tgcttttatc agttatattt
     9361 ttaggtgaag gtttaagttt agcaaaagtt gcaggaatca taattatgat tggtggagtg
     9421 tttatattaa aaaaagtaag ctctttttta aagaatgtct ctgtaatttt taacaattta
     9481 ccatgtagat taatgttttt atacatattt ttgcagtcag taggaagagt attggataag
     9541 gctttttttg tacaagtatc tcctatatta tattcaacta tactgtattt tttcgtaggt
     9601 ttaaatttat ttatcttttt agcatttaaa aagaaacaaa aaataatttg tgagattttt
     9661 agtaataaaa aggggctttc aattttaagt ggtgctatta atggatattc ttatttagca
     9721 ttattaattg ctttaaataa tctagaactt agtatagcag agccattttc tcaagtatct
     9781 atgataataa ctttaatact tgctcatttt atattcaaag aaaatataaa agaaaaaata
     9841 ccaggttcaa ttttgatact aattggtggg tggctactgt tactttaatt gatataaaaa
     9901 atgaattata aaggtgctat tagttagtat cagaaaattt aaaaaagtat tgacaattct
     9961 tttaggcaaa tgttaatata ataatacaaa atagtataaa tagaagacat gttactggta
    10021 atgcaggcag gatcgaatat attgctttga tttttcaagg tgtttatata ggttctgcct
    10081 tttttgcatc ttgaatggcc attcataaaa aattatggag aaggagatta caagatgaat
    10141 tataaaaaaa ctgccattga cattattgaa caagttggtg gttcaaaaaa tattttagct
    10201 ttagaacact gttcaactag actacgcttt aagttgattg atagaagtaa agtcaatcag
    10261 gatgctttaa aaaaagtaca aggtgttatg agtatagtta atggggcaca atttcaagtt
    10321 gtcattggaa atagtgttgt tgatgtgtat gatgaaattt taaaagtttg ccctttagat
    10381 acaaatgata gtactgtaaa aactgaagaa aaaataaact taggaacaag atttatagaa
    10441 tttattatta atatttttca accactgatt tttgctattg caggagctgg tattttaaaa
    10501 tcactgttga tgttattagc aatgtttaat attttaaaat cagattctac tatatataca
    10561 ttattagttg ctatttcaga tgcaacattc tatttcctac ctcttatggt agctgtaact
    10621 actgccaatg tattgaaatg taatcgttta gtagctattg ccgctgtagg atacttattg
    10681 cttccagcaa caacaactgc tttaagtgaa ggtgtcgaac tatttggatt tacaattcca
    10741 aacattgcct ataatgcaca ggttttcccg gctatcttat gtgtgtcttt cttggcaaca
    10801 atggagaaaa tttttaacaa gtattcacca aaaccaattc gaactttctt tgttcctatg
    10861 atgtctttgg caattactgt tccaattact ctaactattt taggtccttt aggatataac
    10921 gttggtacaa tttttacaac cattatttta ttcttatata acaaaatggg attcttagtt
    10981 gtaggattac ttgctgcttt tcttccattt atggttgcaa caggaatgca caaagcacta
    11041 ataccttatt caattaatac aattggaaag ttgggttatg aagctttata tatgacatct
    11101 tcattagccc acaatatttc tgaaagtggt gcttgttttg cagtagcctt gcgaacaaaa
    11161 gatgaaacat taaaacaaac agcattatct gcaggaattt ctgccttgat gggaattaca
    11221 gagccagctt tatatggtat tactttacaa cataaaagag caatgttagg tgttgtattc
    11281 tcaagtgcaa tttctggtac atttttagga ttatttgcag taaaaggatt tgtaattgtt
    11341 ggaccagggc ttgccagtat gagtatgttt attgacccaa acaatggaaa caatttgatt
    11401 tttgcaagta ttggttttgt tattgcaata ttaggctcgt ttattattac actgattatt
    11461 tggaaggaag atacaaatac agtagagtca tcaattggag atgttgttga agaagagcta
    11521 gaagaagaaa aagaagtaaa tatcttagta actccagtag aaggaaaagt aatagattta
    11581 agtaaggtta atgatgaact atttgcaagt aagactttgg gagatggagt tgcaattata
    11641 ccgaccaatg gaaatttata cgcaccttgt gattcagaag ttgtgatgtt atttgaaaca
    11701 aaacatgcaa ttggtttaag aacaaaaaat ggggctgaga ttttgattca tattggaata
    11761 aatactgtaa gtatgaatgg agatggattt aaaacatttg ttaagacagg cgataatgta
    11821 aaagaaggag atttattaat tcagtttgat ttagacaaaa tctcacatgc taatttagat
    11881 tcaactgtta tgattgtaaa caataatgga gcagattatg cttataaagt gttaaaccag
    11941 tcttatggca atgtaaaaaa aggaagtatc ttgtttgatg taaagggagg aatataaata
    12001 tgagaaagtt tccagaagga tttctttggg gaggagctac agctgctaat caatttgaag
    12061 gtggttggaa tttaggtggt aaagggtggt ctgtatcaga tgtagcaaag gcacactttg
    12121 atgcagatgt gaaagattat aaatctaata atgaaatcac aacaaaagat atagaagaag
    12181 gattagcaca tcctgaagat gaagtaaatt atccaaaacg tcatggtagt gatttttatc
    12241 atcactataa agaagatatt gctctaatgg cagaaatggg atttaagaca tatcgaatga
    12301 gtattgcttg gtctcgtatt tttccaaatg gagatgataa agagccaaat gaagaaggtt
    12361 tacagtttta tgatgatgta tttgatgagt tgataagtta tggaattgaa ccacttgtta
    12421 ctatgtcaca ttatgaacca ccattaaata tcgttttaaa ctatgatgga tggtattcac
    12481 gacaagttat caatatgttt gttaggtatg tagaaactat ctgtgaaaga tataaaaaca
    12541 aagtgaaata ttggcttaca tttaatgaag ttgattctat gattcgtcat ccgtacacta
    12601 ctggtggttt agttcgagac cgttttaagg ataaaaactt tgaggaagtt atttttcaag
    12661 ctatgcatca tcaatttgta gctagtgccc ttgcaacaaa aatttgtcat gaaattattc
    12721 caaattcaaa agtagggtgt atgttaacaa aattaacgta ttatccatac acatgtagac
    12781 cagaagatgt tttggcaact caacgaaaaa tgagaagtat ttatgcatat agtgatacgc
    12841 aggtttttgg tgagtatcca gtatatttac tgtcgtattt taaaaataat aacattcaaa
    12901 tagtaaaaga agaacatgat gatgaaatta tgaaaaagta tccagtagat tttatttcat
    12961 tctcttacta tatgtcttct tgtgaggcag ctgatacaac tggattagat attacacctg
    13021 ggaatacgtt attagctgta aaaaatcctt atttagaaat gagtgaatgg ggttggcaaa
    13081 ttgattcaat aggtctacgt atatctttga ttgaattata tgatagatac agaaagcctt
    13141 tatttatagt tgaaaatgga ttgggagcca aagatatttt aactgaagat aaaaaagttc
    13201 atgaccaata tagaattaat tatttaaaag aacactttaa atgtatgtta gatgcgatta
    13261 ttgaagatgg tgttgaactt tggggatata cttcatgggg ttgtattgat ttggtcagtg
    13321 aatcaacgaa acaaatgtca aaacgttatg gatatattta tgtggatgct gatgattatg
    13381 gaaaggggac atataatcga tataagaaag atagttttta ttggtataaa aaggtaattg
    13441 aaagtaatag tatagatttt aaatgaccaa tagaaggtga gcaaataaaa tgaactacat
    13501 aataaagaaa gttttgaact cgagcgttgt tttagttcat gacacaaagg ataatgaatt
    13561 tattttgcta gggaaaggaa ttggatatgg taaaaaaaca ggagaatata tttgtggttc
    13621 caatgataat caaatgtttg tccctgttga aaatacaaaa tcaaaacaat ttttagaatt
    13681 aatggatgat attcctgtcg acattttaaa gataacacag gaaataatag tagaggcaga
    13741 aaagttattg aactcatctt ttaataaaaa cttatactta atattagcag accattttaa
    13801 ctttgcaatt gaaaggatgc gtaaaggaat aaaaattaca aatcgagtat tttgggaaat
    13861 aaaaaattat tatccaaatg agtttaaggt tggaatggtt gcaatttata tggtagagga
    13921 gagattgggc attcaattac cagaggaaga agcagctaac attgctttcc attttgcaaa
    13981 tgctatggct tcagatggaa attcatatga tacgattaag tatgccaaag taattggaga
    14041 aataattaat atttttgttt tttctttgaa tagaacctta gataaaaaga gtatgcatta
    14101 tatgagattt ataacacata taaaattttt tgtggaaaga tttttttcag atagtatgtt
    14161 aagcagtggt gatgatttat tatttaatca aatgaaaagg tcaaatccaa aagaaatgtt
    14221 aattgcatta aaagttagag attttttaga aaagaaatat ggaaaaaagt taacaaatga
    14281 ggaaatagct ttcttggttg tccatatcgc acgagttaag caatagagtg aattactaat
    14341 attatcttat acgtctacat ggtagaaaat tgaaagaagg taaagtatat
//
LOCUS       DACULQ010000099        14373 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.099, whole genome shotgun sequence.
ACCESSION   DACULQ010000099 DACULQ010000000
VERSION     DACULQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.099"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            147..368
                     /locus_tag="JA154_11865"
     CDS             147..368
                     /locus_tag="JA154_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996550.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            368..631
                     /locus_tag="JA154_11870"
     CDS             368..631
                     /locus_tag="JA154_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HAT7996551.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            693..1265
                     /locus_tag="JA154_11875"
     CDS             693..1265
                     /locus_tag="JA154_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAT7996552.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(1731..2050)
                     /locus_tag="JA154_11880"
                     /pseudo
     CDS             complement(1731..2050)
                     /locus_tag="JA154_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429539.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(2019..2369)
                     /locus_tag="JA154_11885"
                     /pseudo
     CDS             complement(2019..2369)
                     /locus_tag="JA154_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2686..3219
                     /locus_tag="JA154_11890"
     CDS             2686..3219
                     /locus_tag="JA154_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-O-methylguanine DNA methyltransferase"
                     /protein_id="HAT7996553.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPDFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGINRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            3445..3624
                     /locus_tag="JA154_11895"
     CDS             3445..3624
                     /locus_tag="JA154_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT7996554.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNNYNIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4232..4480)
                     /locus_tag="JA154_11900"
     CDS             complement(4232..4480)
                     /locus_tag="JA154_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996555.1"
                     /translation="MEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVI
                     KHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            4659..4889
                     /locus_tag="JA154_11905"
     CDS             4659..4889
                     /locus_tag="JA154_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996556.1"
                     /translation="MFSIPLILPCILLIVPSIPLILLCISLIVPSIPLILLCISLIVP
                     SIPLILPCISCNNSNIFSSITITLLFYYYNII"
     gene            5315..5728
                     /locus_tag="JA154_11910"
     CDS             5315..5728
                     /locus_tag="JA154_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996557.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            5801..6337
                     /locus_tag="JA154_11915"
     CDS             5801..6337
                     /locus_tag="JA154_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HAT7996558.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNGVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            6497..7159
                     /locus_tag="JA154_11920"
                     /pseudo
     CDS             6497..7159
                     /locus_tag="JA154_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7393..7542)
                     /locus_tag="JA154_11925"
     CDS             complement(7393..7542)
                     /locus_tag="JA154_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996559.1"
                     /translation="MQAQTISYPSALSVKVYPLPLPIACSVLPSRGIVVEVSPIRSLP
                     ADCPF"
     gene            7705..7824
                     /locus_tag="JA154_11930"
                     /pseudo
     CDS             7705..7824
                     /locus_tag="JA154_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7951..8853
                     /locus_tag="JA154_11935"
     CDS             7951..8853
                     /locus_tag="JA154_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HAT7996560.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            9465..10478
                     /locus_tag="JA154_11940"
     CDS             9465..10478
                     /locus_tag="JA154_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HAT7996561.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKIFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPSDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            10834..11403
                     /locus_tag="JA154_11945"
     CDS             10834..11403
                     /locus_tag="JA154_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HAT7996562.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYFNRYN"
     gene            complement(11739..13076)
                     /locus_tag="JA154_11950"
     CDS             complement(11739..13076)
                     /locus_tag="JA154_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT7996563.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYTLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMIFYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(13520..14230)
                     /locus_tag="JA154_11955"
     CDS             complement(13520..14230)
                     /locus_tag="JA154_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996564.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT         5238 a         1646 c         2364 g         5125 t
ORIGIN      
        1 agaggtaaaa taagaaaaaa agatacttat ttaatcattt aatcttatta aagacctatt
       61 aaaactacca gttataatat agaaccagta tacaattttt aaaaataatg ctattttaaa
      121 attctaaaaa ttaaggagga taattaatgg gatatttaaa agaggataaa caagactatt
      181 gttgtgaatt tgctagacca tacatcaatc gtcaaattta tgatgacaca tacaatccaa
      241 aaacagcatt aaaaagaggt actttatttc ctgaattaga tttaatagag tcaaataact
      301 ataatgattg gctatatgca aatcctaaaa atagagttag agctaaaaaa gggggtaaca
      361 tgtactaatg tcattaaata gaagagaact tttagagaga atttcagaat atcaatttgc
      421 ctgtatagaa ttaaatttat atttagataa caatccaagg gataagaaag ctcttgattc
      481 atacaacaga tactgcgata aatttacaca agctgtgtgt gattatgaat ctaaatatgg
      541 agcattaact aattttggat atgaatcaag tgaatatcct tggtcatgga tatctgaacc
      601 ttggccatgg gacaaatcat tttataaata aaattattaa actttaaaat tagatttatt
      661 attaaatata tattaaggag gagtaagata atatgtggat ttatcaaaaa acactggaac
      721 atccagttaa cataagacaa gcagacccta gaatggcaaa atatatcatg actcagttgg
      781 gaggacctaa tggtgagttg gcagctgcaa ctagatatct tcaacaaaga tatactatgc
      841 caactggaaa atctcgtgca cttttaactg atataggtac agaggaaatg gctcatgttg
      901 agataatttc ttcagtgtta tatcaattaa taggcaattg tactccagaa gagcttaagg
      961 ctgctggact tggtagtaat tatgctaatt ttggacatgg tcttcagcca gtagattcta
     1021 atggagtaaa ctttactaca agttatatta atgtctttgg cgattcggta actgatttac
     1081 atgaggatat ggctgctgaa caaaaagcat tggctacgta ctatcaatta ataaatttaa
     1141 ctgatgaccc tgatttgaaa gatatattga gatttttggg tgagagggaa gtagttcact
     1201 atcaaagatt tggtgaagca ttaatggatg tttatgagtt tacagagtgc aagcatcagt
     1261 tttaatgaag aattgaaagt gaatggagaa taagcatcat ttttaatgaa gaattaaaag
     1321 tgaatggaga gtaagcatca tttttaatga agaattaaaa gtgaatggag agtaagcata
     1381 agttctaatg aagaattaaa agtgaaaaga gaataagcat aagttctaat gaagtattaa
     1441 aaatgaaaag agaataagca tcatttttaa tgaagtatca aaaataaaaa gagtgtaaat
     1501 attagctctg ataaactatc aaaataagag gtaaaagttt tagttgattt cttttatctc
     1561 ttatttttta ttgattaaac agattacttt gctatttaaa tatattattt tagcaaaaaa
     1621 agtccaatca aaagttcaaa gtattaccat agttcaaagc attgccgttg ttagtaagtt
     1681 ttttacaata ttttttattt actctttgga taatcaaaag aaataaaaaa tcatatctct
     1741 ttttttatat ttctgtacac ttttggagaa tatcccgttt tttctttaaa acatttacta
     1801 aaatagatta tttgatgaaa aacaactttt atagtaatgt cacttattag ctcatttgtg
     1861 ccttttagaa attgagttgc ttttaataaa caaaactcag taaaatattt ataaggtata
     1921 gtatttaaac ttagttcaaa tcatctggag cactctgatt tattgatatt accactgctt
     1981 gatagtactc caaaattaag ttttcacaat agtacccttc aatataatgt aaaacaatag
     2041 tatttttcaa tataacgtaa aattttttgc atatggatat taattatatt tttgtgtttt
     2101 attggtggca aaacaatatt ttttatcata ataagccaaa ggaaagaaac agaaactaat
     2161 gcttcataca tatacagttc atttttactt ttttctaacc ttattaatcg ttgtagaata
     2221 gaaaacactt tattgcgtca attatattta tgtgtaaaat gataaatagc aaattgttcg
     2281 ttttctgtta ttctatctac taacgatttt actggatttc taacataaaa atacttttct
     2341 tttttacagc ctattcttac ttttcccatt tgaaagtatt atgatattac ttgatgctat
     2401 atcgatattt ttttggtcta tttagtgata aaattataag atacacttct ataaaagtta
     2461 atacagtagg aagcttaaat gtatgaattt tatatccatc aaagggaaag ggaaaaaagg
     2521 atgaacagat gcaggtaaat ataaaactaa tatgatatga ggatagatat tataacgaaa
     2581 gtgtagttag aaggaaatta tattagtatg tttttacata atggtagata gaaaagaagt
     2641 gtctaatgat ttgagtgaag ttttcagaat ataaggagga attgtatgca atatattagc
     2701 tattaccata gtccaattgg aaatattctt ttagcatcag ataatattgg gttaactggt
     2761 ctttggtttg agaatcaaaa gtattatgct tataatcttg accaagaaca tcaagaaaaa
     2821 gaattgccaa ttttcgagca gacaaagaaa tggttagata tttatttttc aggaaaagaa
     2881 cccgatttta caccatcact ccatatggta ggtactcctt ttcagactac agtgtggaaa
     2941 attttacaac aaataccata tggtaaaaca atcacttatg gagaaattgc atataaagtt
     3001 gctcaacaaa aaggaatatc taaaatgtca gcacaagcaa ttggtggtgc agtagggcat
     3061 aatggaatct ctattattat tccatgtcat cgtgttgtag gaacaaatgg gagtttgact
     3121 gggtatgctg gtggaattaa tagaaaaata aaattgttat ctttagaaaa agtatctgtg
     3181 caacattatt ttatcccaaa gaaagggagt gcattataaa gccattaaat ttagaaattc
     3241 ttaatcattt atttgatata caaacatcta attttgaaag caataacttg caattttaaa
     3301 aaatgattat ttaaactata tgttatacaa ccttattcct agtaaacatt atacaacttt
     3361 agaggtagtt tttagagctt gtattttgga agtttatttt cttttttcat gtgagtagtt
     3421 atatgtttag aggaagtaat ttatatgcta caaacaggaa aacaaggagc agacaaaaaa
     3481 tacttagata atatgttttt tgtgaaaggt tatgttgaag aacttctatt tttagacaac
     3541 aattataata ttgtattttc atgtattgta tttcatcatt gtacaaatgt tcaaaatata
     3601 ttttgtatat tttggaaatg gtaagagtgt taaatgtaag ggaaaaattt ataatgattg
     3661 atacttaagc tactcattaa gtattaagaa ccatacaaga taaggttgaa atttatatgt
     3721 ttcgttttat gtaaaaaatg taagtgagtt agaaatgaaa gaattattta taaaaaatag
     3781 attaagtatt ggaaaatatg aaaaatagat gttgaacaaa acttagtcga ttagttaaat
     3841 ttaaccaaac acttaaagta tcaaaagaaa aaattattac tcttaagcaa gaagatataa
     3901 atggaaaaat gaaaacaaga ttttatcctt ttataagtga taaggggatg tgttttaatc
     3961 aaagatggac ttttatttta ggctgaaaaa ttatataact atattatagg atataaggga
     4021 gatacacaac tttttagtgt ccaatctccc ttatttttta ttattatatc aagactcatt
     4081 tagtcgtgtt aattttagga taaaatgagt atttttttaa taaattattt ataagtaaag
     4141 tgtgttgtcg gataatcagt atttctgtat aaaatttggt ataactttaa agaagtatgt
     4201 gaaattttca ggtatttttt actatggtat attatttaac tgattttaat ttagcaatat
     4261 cattcgaatt cttagatgtg actatctcta tattatttaa atcattttta atatctgatg
     4321 tatcttttac taatgaagag gtatgtttaa tcactgtatc taatgtattt ctgttaattt
     4381 ttacatctga cttaatttct gtaacatcac tctgaatagt atcaagtcta ctactagttt
     4441 caacttgtcc agtttttaag tctttaatat caggttccat attgtctaac ttactattga
     4501 cttctttaat ttcaacttgc atatcagtaa tactgtcttg catactatta atagtgccat
     4561 ccatactact gatactgcct tgcatatcgc taatagtgcc attcatacca ctgatactac
     4621 cttgcatatc gccaatagtg ccttgcatac cactaatagt gttttcgata ccattgatat
     4681 tgccttgcat actactaata gtgccttcga taccattgat attgctttgc atatcgctaa
     4741 tagtgccttc gataccattg atattgcttt gcatatcgct aatagtgcct tcgataccac
     4801 tgatactacc ttgcatttcc tgtaataact ctaatatttt ttcatccatc actatcacac
     4861 tcctgtttta ttattataac ataatttagg tgttatattc cacaaacgct atgtcatatt
     4921 taatttttaa tcatatgatt atctgctatt caaaacatgg ttaaatatat ctatattgag
     4981 aactttttta ttaatgaatc ttatgtattg tctaagtttc taagatactg taataagtgt
     5041 ttttgaggca tgtgtggtta tatttgtatt taaaactgct ggtaatatca tatactttta
     5101 aatatacctt aagttattta tattgattac ttgtatttga ataatttgat tagtagcttc
     5161 ctttttatct ctttttattc ttagttttaa actttcatat ttccttgaaa atttattggc
     5221 aatgaaagtg catgaatttt ttagctattt tttatgttac aatatgaata tataacaatt
     5281 cgacaaattg gatttttgaa aggagtaaaa tgttatgaaa atgccaaaag agagtattga
     5341 tacgattatg tttgctcctt gtggaatgaa ttgtatggtt tgctataagc attgttatca
     5401 caaaaagcca tgtgctggtt gtttgaatgg cgatacagga aagccagaac actgccgtaa
     5461 gtgtaaaata aaggattgtg tctgtcaaaa agggttgttt tattgttttg aatgtttcaa
     5521 ttttccttgt aaactcataa aaaatcttga aaaaagttat aacaagagat atcaagcaag
     5581 ccttatggaa aatagtaaat ttgttcagca gtacggttta gagaagttta tgcaaaagca
     5641 gaaggagaga tatacttgtt ctaaatgtgg aggaattata tctattcatg atagggaatg
     5701 tagcgagtgt caagaaaaag tgaaatagct aaatcactgt ttatcaagag agaaaaaatt
     5761 gtaatatata taaaggtgga ttatattgag gtggtatata gtgataggtt taaaatgtgg
     5821 tatggttaaa ttattggaac atcagatgat atgggataaa agtgcaaaag atgtcattat
     5881 attattgaaa agtatatggg ataagactgc cattgatatt caacacattg gaagcacttc
     5941 tattcccagt attagcgcaa agcctattat tgatattgtt gtaggtgttg cttctttaga
     6001 ggaagctaaa ttatacctcg aaaggttaga gcaatgtgga atcgtttttc gaggtcagga
     6061 tgtaccaaaa caattacttt ttgctatggg agattttgag aaaaatactc gtactcacca
     6121 tattcatgtt gttgaatgga atggtgttgc atggaataat tatatcaatt ttcgagatta
     6181 tctaaatact tttacagaca aagcaaaact ctatgatagt cataaacaaa aattggcatt
     6241 acagtttgag gacaatcgta aaatctatac acagagaaag caagaactaa taaatatttt
     6301 gctggaagaa gcacgactgt ggagaataaa tacctaaaag aataaattta catttattga
     6361 gaaattgagc aattattttc ttatatttag taaataaaaa tctatttttg ttgattaaaa
     6421 aatatctgta cacactcatg actttagtca ttggagattc agattgtgta gaattattgg
     6481 atgaaggagg aacagtatga aaaaggatac atctcatatt atcaaacggg cagagcactt
     6541 tcaggtaatt ttgattggtg aaggacttat ggttggggct gtggctggtt tgattgtgct
     6601 tttatacagg atacttttag ggcaggctgg tacttggctt agccaaattt taaaatttgt
     6661 agaaggttct acaataaaaa taatagcttg gtttgcagta cttgcagtac ttgcatggat
     6721 tgtatcaaag ttggtggact gggagccaat gatttctgga agtggtatcc ctcagcttga
     6781 gggggaaatg acagggaaaa taaaacaagt atggtggcga gtgttgccaa caaaatttat
     6841 tggaggattc ttatcactca tgggtggtct tgctttggga agggaaggtc cttccattca
     6901 gcttggtgct atggcaggta aaggtatttc tagagctctt gaccgaggga agacagagga
     6961 aaaattcctg ttaacctgtg gggcaagtgc tggattatca gcagcctttc atgcaccact
     7021 ggcgggtgtt atgttttcac ttgaagaagt ccataaaaac ttttcagtat ctgttttaat
     7081 ttctgtgatg acagcatctt tgacagcaga ctatatttca gccaatttcc taggaatgga
     7141 gtctgtattc cagtttgatt aaaaaatagc tacgcacact tgtgacttta gtcatgagtt
     7201 agtagctaaa atagtcagca tatagggaaa cctatatgta gagataggat agaacctatc
     7261 caatactact tgaattgctg gaaaccccta aagctaacca aactaaaaca taaggatgaa
     7321 ataaacctaa gtgtgaaagt tacgaaagta gaaaaaattg gttagatggt ataaggttaa
     7381 atcctaagta ctttaaaatg ggcaatcagc aggtaaactc cgaataggag aaacttcaac
     7441 gactattcct cttgagggaa gtacactaca agctattggt agtggaagtg ggtagacctt
     7501 aacggataat gccgaaggat aagatatagt ctgtgcttgc atgaaagtgt aagaagttca
     7561 taagagaact gcataggtag tagcgaactt atgtgaacga caacctcaaa aatgataaaa
     7621 gttaagactt acggttctta aatatgttat ttaaaattaa atataaaacc ttctaaataa
     7681 ttgttttaaa taattacatt tgttaacatt attataagag aatggatttg tcctagttgt
     7741 aatgaaactc acgatagaga tataaatgca agtataaata tattaaaaga aggattaaga
     7801 ctaataacaa ttcaaaataa ataacttatg taagaaccgt aggaactacg gggatagcct
     7861 gtggagaatt agtaagacat atgttagtat gcaaaattct atgaagcagg aaccctgtga
     7921 ctttagtcat gggaggttca gattggaaat gtgttgccac aagattatta tggattaata
     7981 gtgattttag gtgttattct tggagttatg ggggcatttt acaactggtt tactctatat
     8041 gtgcagtctt tatacaagaa gattcctaaa attggtacgt ttggaaaact gttgattcca
     8101 tttataatgg ctggtattct tgggcttgtt atgcctgatg ttctggggag tggacacaat
     8161 ttagttgaag aacttacaag tcatgaatat atttttggaa tggttgtttt gatttttgtt
     8221 atacgtttta tattttcggc tgttagtttt gggtcaggtg caccaggggg aatatttttt
     8281 ccattgcttg ttttaggagc atttataggt ggaatatttg gtatggtagg tgttaaagtt
     8341 tttggaatga atcccgatta tgtaaataac tttgtgcttt tggctatggc aggatatttt
     8401 acagctattg tgcgtgcccc tttgactggt atcatattaa tttttgaaat gacaggttct
     8461 gttagtcaaa tgctttcact atcagtaatt tcaattgtgg catatattgt ggctacactt
     8521 atgaagtcta aacctattta tgaaagtctt cttgagaggt tacttgaaaa aaatggagaa
     8581 cctgtacata aagagagggg cgaaaagata ttagaccagt tctctgttat gtttggctct
     8641 cctgttgcga acaaggcaat tcaggatgta cagtggcctg aaaattgtct tttggtagca
     8701 attcaaagag gtggagaaga aattattcct agaggtaaga caattcttag accaagtgat
     8761 gtaattgtga ctatgacaga tgagagagat agtggtgctg tgtatgataa gatggaaaat
     8821 ttgtgtaaag aacatacgtc acatattttt tagggataat gatgaatgtt taataatgat
     8881 agaatattta atgaagattt gattaaaaaa ttggtaattt gaaagagagt taaggaatta
     8941 atataagaaa atgaaaatgt gatatcttag atgtaaaaga gtttgaagaa aataattgta
     9001 tatatttgtt aatgagatta tatggactaa tggtatattt atactgtgct aaaagttgca
     9061 ctattgtatg aggaatggga ataaagggtt tgaaaaaata ggtagaagat ttttcagata
     9121 ttcttactgg gatattttaa ttaatatatt ttaattgcga tattttggga agaatagaat
     9181 ttatttataa atgaaaataa ttaaaagggg ttaaggttgt attagcccct tttgcttata
     9241 gagtttggaa atggtatctt gatagcagtt gtttcatgct tattatattt tattacatat
     9301 tttaaaattg accttattac tcctaaatca ttgttatatc aacttttaaa acactttcaa
     9361 gacaatcgcc tattgaaaac aaagttaatt aaaaacctac atatcagttt agtattcgtg
     9421 tagatgatta tataaatata aaagtataag aaaggtgagt atctgtggtt agttcatttt
     9481 taaatgatat ctttagaaca attttagtaa gttgtatttt tatagttatt ttattagtct
     9541 ttagaataac tttatttaag attttcagta aaaaatttaa ttactatata tggcttattg
     9601 tgattattaa gctatcatta ccatttatga attatacttt tatatttaat gagtcaaaat
     9661 ataatggtaa tattaataag ataagtttag gcaattttaa tagtatttct attgtatata
     9721 gtactgtttt agtctctgtt tggattgcta ttacgatatt ttatttagtt tatactttat
     9781 tgaaatatat taagcttaaa aatatgataa atgatttgtc ttatgatgtt gaggatgata
     9841 agataaaaaa tctttatgaa aatttattaa aagagcttaa gataaataaa aaaatacggc
     9901 ttaaatattc atatgaagtt gaaagtccag tattttttaa ttcgtgtgtc attttaccac
     9961 caagtgatta tacattaaaa gagttaaact ggatatttag gcatgagttg atgcatttta
    10021 agagtaaaga cctttatatt aagtatttgg tgatattttt aaaatgtgtt tattggttta
    10081 atccttttgt gtatataatg gagaagttta tagatttgga ttgtgaattt tattgtgatg
    10141 aaagagtttt gaagaattgt acgatagaag agaagcagga ttatgcattg acaataatta
    10201 gtgcaatgag aaaaggttca aatagttcaa ataaatttgt ggctggatta cataaggagt
    10261 cagatattaa aaaaagagtg tttagtatgt ttaatgaaaa gtacagaaat ggtattttga
    10321 tagcagttat tttatgttta ttatcttcta ttacatattt taaaattgat ttaattacta
    10381 ctaaatcaat attttatcca ctttcaaaac aaacacctat tgaaaacaga actaattcaa
    10441 aacctacata tcagattaat gtttatatag ataattaaaa aatacaaagt ttaacaagag
    10501 attaatataa ataattttaa taagtatctt tcacatatgt taaatttact ttaattatat
    10561 taaagccaat gaattattct ttagaagctc caatgccagt aaatacaggt aaagaaaaat
    10621 attttactac aattgcagat taaaaagtgt tctaatattt aaagaaatac acaagtgtaa
    10681 gaagggtaaa aatctgttat aatacattaa aaactaaatt tagaaaaatt tacataatta
    10741 gaaaaatgat ttgataaaaa ataaagttaa atattaacta agtaaatttg aaaaagaaaa
    10801 aatataaagt acaaatttaa aaggaggtaa ctaatgaaaa agaagttgga agatatgact
    10861 ttagaggagt tatggaactt gtttcctata tttttgacta ctcacaaaga atgttggaaa
    10921 aattggttta aagaagaaca atatagacta actggaatat tgccaatgca aaaaattgtt
    10981 agaataagtc atattggaag tacttctgtg agtacgattt atgccaagcc cattgttgac
    11041 atactgattg agataattcc tgattataat atgaaagata ttaaagattt gattgagtcg
    11101 aatggatata tttgtatgtc tgagagcagt agtaggcttt cacttaataa agggtataca
    11161 gtagatgggt ttgaagaacg agtttttcat ttgcatataa ggtatgtagg tgataatgat
    11221 gagttgtttt ttagagatta cttaattgac cacccagatg ttgcaaagga gtatgaaaaa
    11281 cttaagttgg acctttggaa gaagtatgag cataatcgtg atgcttatac agatagtaaa
    11341 ctagattttg ttagggagtt tactgaaaag gcaaaggtgt tgtattttaa tagatataat
    11401 tagaaatagg tacaattaga tatttatctt atatatcgta ttatattttt taagaaagta
    11461 attaatagaa aaaggggttg tttcaaaatg gagagataaa tccctttttc attgttgaaa
    11521 ggataaaaat atatttgatt tcatgtaaaa aagagtgcct catcaactaa aaagtttatt
    11581 ttgagacaca cacattttta ctaaggtaaa taaaatatca catttgatta aattttaagt
    11641 taatgaatat atgtgtaaat atttaattat aagttattat aattttacat agtatcaaac
    11701 aatctaattt gtattatata agataatcta attttagact aaaataatct aatataaaga
    11761 aataaagaaa attcattctc agtataagag atatccaatt ttccattgta cttattaaca
    11821 atttctctaa tattctcaat accatatccg tgagaattac tatctttttt atctgaaata
    11881 aatagttctt gttttctatt gataggattt accttgttgt ttgtcaataa atagctaaaa
    11941 tactccttct cacaacgtgc ctttaatacc acttttctat ttgacaaatc aggaatcttc
    12001 aaagatgctt ctatagcgtt atctaaagaa tttgaaaaaa tagtacataa atctataggt
    12061 tctataccca agtgattgtc tatatctata ttaaagtgca catcaacacc attttcttcc
    12121 aaacttgtat gacgaagatt taaaattgag ttaagagctt ggtttttgca aaactgtttg
    12181 ccactaagag tacctatacc tatttttaac tgattaaaat actttttagc tccagcaata
    12241 tcttcaattt ctaaatacga ttcaatcatt tgaaagtgat tattcatatc atgtcgcatt
    12301 ttgcgtattt gaatttgttg attttctagt aagttatagt atttttcttt tagatgattt
    12361 gcttgttttt ctatcttcat ctttacattt tcaatcaatg gctgtaaaag aagtatcaca
    12421 ccgatatttg ccagaattgc tgacaagatg attagataag tgccataagg taagttgcct
    12481 aagataatat tcttattact acccataggg attaaattag taggtggcag aataatagca
    12541 attaatattg ataaaaatga agatatacat accatatcaa taaaaatcca tattttattt
    12601 gataagtatt gttttatatt gaccaatcta ttctttagga aaatatgtat taaaaaccaa
    12661 aataataatt taattagagc agaaattatc tggactattg aaagtattaa aatatactct
    12721 tttgagtgta taagttcctc atatataggg ttgtttataa aaagaaaatt aagcccaatt
    12781 ataataggat aaaatatcat gacaactgat attataggaa ttaatttgct tttgctacat
    12841 attataagta gtataaaata tccgaatagt gtaaacaaaa tatttacggg gtcggaaagc
    12901 catattatca tttgactaat aatagagtag cccaaaatca atatgatttt gaaaatccaa
    12961 tgattcctaa agtctataaa ataggaagtc aacgtataaa gaaaaaaggc tatgaaaata
    13021 ttgattaata ttagaataaa atttatgctt gtagagatta aagaataagt gcccatttaa
    13081 tatacctttc tattaattaa gattctggaa actaagtttt aaaaaattaa atcttaaaaa
    13141 ttaaaattct ggaaattaaa tttcaaagaa ttgattttga aaaataaaat tctagagatt
    13201 gaacttcaaa aaattgagtt ttaaaagatt gaaattttaa aaattaagtt tcaaaaaatt
    13261 aaatttgaaa aaaaactcta aaaactaagc ttcaaaaaat taaattttga aagattgaaa
    13321 ttctagaaac taagctttaa aaaattaagt ttgaaaaaac aaaactctaa aaactaagct
    13381 tcaaaaaatt aaattttgaa agattgaaat tctagaaatt aatcttcaaa aaattaaatt
    13441 tgaaaaaaca aaactctaaa aactaagctt taaaaagtta agtttcaaaa ataaaattct
    13501 agagattgaa cttcaaaaat taaggttcta acatatacct tgtaaatttt cctacaacct
    13561 cttgatattt tttacggctt ataggaattt tttcattctg aactttggca taagtttgag
    13621 ttatactatc tacatggttt agattgacaa tatacctttg atgcgtacgt ataaaattag
    13681 agggaagaac agtttccata tcatttaatt ttccgtagta ttctaatgtt ttatcaaagg
    13741 taactacagt taattttctt agctcacttt taaagtagag gatatctttt gtattaattt
    13801 tacaaagatt agttccaatc tgaactgtaa taaagttttc tttccattgt tctaattttt
    13861 ttagtgcttc ttttaatact ttacatattt tttcagtttt ataaggtttt aagatgtagt
    13921 tcaaagctcc tacatcatat ccatgaaata catagtcaga aaaacctgtg acaaagatga
    13981 ttatagtatc ctttgaatgt tttcgtatag tttttgctat gtcaacgcca ttactttttc
    14041 caagttcaat atctaaaaaa ataatatcta tgtaataatt ttcgtgttct agattgtata
    14101 ctagactttc tccatttgaa tattctttta tttggtaggg gtagccccac aaatccattt
    14161 ctcttgtaat gattttattc agcatgtttc tttgttgttt ttcatcatca caaatacaaa
    14221 tcgaagtcaa ttttttatct cctttcaaat tttatattta tatagttaag tatataatat
    14281 attttgctac ctagatatat aaaatgaaat aacaattata tccagatagc aaaataaaat
    14341 tttttatatt agtatactta cacttaaaac tat
//
LOCUS       DACULQ010000100        14330 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.100, whole genome shotgun sequence.
ACCESSION   DACULQ010000100 DACULQ010000000
VERSION     DACULQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14330
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.100"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            2..421
                     /locus_tag="JA154_11960"
     CDS             2..421
                     /locus_tag="JA154_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996565.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            446..5503
                     /locus_tag="JA154_11965"
     CDS             446..5503
                     /locus_tag="JA154_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAT7996566.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(5689..6426)
                     /locus_tag="JA154_11970"
     CDS             complement(5689..6426)
                     /locus_tag="JA154_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996567.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(6445..7380)
                     /locus_tag="JA154_11975"
     CDS             complement(6445..7380)
                     /locus_tag="JA154_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT7996568.1"
                     /translation="MANNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRVHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            7588..10164
                     /locus_tag="JA154_11980"
     CDS             7588..10164
                     /locus_tag="JA154_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996569.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVILANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            10176..10847
                     /locus_tag="JA154_11985"
     CDS             10176..10847
                     /locus_tag="JA154_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996570.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            10874..11215
                     /locus_tag="JA154_11990"
     CDS             10874..11215
                     /locus_tag="JA154_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996571.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(11575..14217)
                     /locus_tag="JA154_11995"
     CDS             complement(11575..14217)
                     /locus_tag="JA154_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAT7996572.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         5277 a         2067 c         2067 g         4919 t
ORIGIN      
        1 tatgcaaatt tattttagag ttaaggctgt tggcaaacgc aaacctatgc tagaacttac
       61 cccttttgat gtatctgaca acatcaattg cctaaaagaa gctatctctg aaattgtatc
      121 taaaaatgtt cttgaatata atgagaaaat cccagaaaaa tcaataataa acttcttaac
      181 taatgaagaa attgaatctc aagctgatat tggaaaagtt ggatttggct ctatttacaa
      241 tgagaataaa caatctgtag aaaaggcaat agaggtagcg ttacaggcat ttgaagatgg
      301 aatctacaaa gtgcttataa atgaagatgt tattgaaaaa ctcgattcct ctataaatct
      361 gaaaagtgga gatatattta cttttataag acttaccttg cttgctggaa gaatgtttta
      421 aaataaataa tttggaggta cataaatgtt agaacgtact agagtagaaa aaaatcctaa
      481 aataattgaa aattttaaaa ataattttac taaatcttct tcaaaacagt actacgactt
      541 agcaaagtct ctttgggact gcatagaaaa tagttcatac tattatagag aacctgaaga
      601 caaattctta aatatgataa agaaatacta caattgccac tactacgaaa ttaaaaatat
      661 ggacgagctc ctttctggac ctttaaaaaa tgcattagaa ttaatattta cttctgaaca
      721 agtagaagaa ctttgccaat taattaaaag aaagatggat tatacatatt caggaggata
      781 ttatcgtaaa gggtatcgct caaataaact tgtggattat tttagaggta ttataaggtt
      841 tatttcaaac tatgttgatt taatttatta tgatttttca gttatagatt atcttactct
      901 tccaaataag gtcttgtact atgactctat aatgagtgat aaaatagcac tagaaataga
      961 caaaggaaat caaaaagtaa taagtgcttt agaagaagta atatttggag aaaatcaaac
     1021 tgctctactt acaagacaaa tgataattgg tatcatgaag agtcatagtg ataaatgcta
     1081 tgaaatgctt tctaaacttc ttttggcagc caaacaacaa gaaggactta gacagcaaat
     1141 tgtagagagt ttagatgagg gtacaatttc tgcatcaata tacatgttaa aaactatttt
     1201 ggacaacaat ttagagcgat ttagttctgt tataagagcc ttggatacat ggacaggact
     1261 tggtttttca gaccaaaaac ctggaataat acgtaaatgt ttagaacttt catataaatc
     1321 attgactgat gaaaagttcc gtatagaatc aattaatagc aatgataatc ttgaaattta
     1381 ttttgcctta tgggctactg caacttacaa catatatgac tcttactctc ttattgaaaa
     1441 cttactttct tgtcaggaaa aatataaaaa acttgtagct ctttactttt tatcgcatac
     1501 agaaagtaca tattttaaac attctatagc atctaaaaac attaagggtg atgatttaga
     1561 gatactagca tgggtaatta caaatttcta tgtagaaggt tctgttggct actactatag
     1621 ttggcaaaac agagaaacta attttgactt gtcaaacact cctattgaca aagactttga
     1681 tgctagaaaa gaacaatttg aaagtttaaa acatacttta gaaattttaa attcaaagac
     1741 aaaatcattt tcaggaagtg tatttccatg gacttggatt gaaatttctt ctcatgaaat
     1801 acttaaatgt atgatgactt taacagcata cgatatgaat gctcaattta tagatgtgct
     1861 tatggaaaat tgtcatctta tgagttcaga tttaagagct tcacttatag tcaattttgt
     1921 aaatgaacca aagacagata gacaacgtga atttttattg gaatgtttaa gtgataagag
     1981 tgtttccaat agagaaacgg tattgagtct acttagaaag cttgagctaa cagatgtaga
     2041 attagataag ataaccaatc tattgtcttt aaagacaagc tctattagac aaacagtaat
     2101 aaaaatttta cttgaacaag atgattcaaa tttggaaaat attgcttctt cactgataaa
     2161 atcttcaaat tctcaaaaat gccttggagg tcttgaaatt gtaaacttat taaaagacaa
     2221 tactgctaga caagaactat ataataagct tgttttactt atagaagata ttgataaatc
     2281 taaaaagaat acatctcaag ttcaaatact aattgataat ttgttaggta aagcaaaaaa
     2341 tgattactgc aaagaaaatg gttttggaat atataaccct gaaaacaaag ttcaaattcc
     2401 agaacaattt gattttaaag atgattctaa tagctcatca aagcttaata aaattatctc
     2461 aaatataagt ttatctactc gtgaggattc tgtttctgta gcaacaaaaa aagaagcaaa
     2521 aaaaattctc gatacggata taaataaaat aataaaagtt tatgaaaaat ttgatgagct
     2581 tattactgaa aatgcagatt atgaatatga agttgaaaga tggtcaggta atgtagacaa
     2641 agtactttta ggttattcta cttataactt gactcctatt aaatataaag tttctaatga
     2701 tactcttgat aattatccat tagcagagct ttggagagaa actagtaaaa agcttgactt
     2761 agattacaaa ttaatacttg atattttatt ttatagtata agtacatgtg gatataatga
     2821 aacagaaaaa agttggtttt catctatata ttcaaatgtc cttgactcta aaaaacaaaa
     2881 taaaatatat acaaaacttg aaaaacttaa atttaatgga cttacaagaa aacttttaag
     2941 agttttacta aatgagtttg accataaaga aatatttaac tattgtttag aaatatacta
     3001 taaacttaga aacttattga ctatagaaca atgtaaaaaa caaagagttg aatttactaa
     3061 aaactattac agacaaaatg aatactgccc tgctttaaat gcccctattt taaactattg
     3121 gcttgataaa gcacattcgt ttatttatga tgatatttct tttactaagt ttttctacac
     3181 tacgttttca aattatcagt tgctagaata taagtttcct ttctatacta gcataaatgt
     3241 gtttgctcgt gcacatgaat taggattaat gactgaagag caagttaata gagaaatgat
     3301 aagtggatac aacgcatcaa atataataag aacattgaca aatcctagat gggctggcga
     3361 cacattagaa aagtaccctt ggcttaaaga taatttaaaa actgttacaa accgaattat
     3421 agaaattgaa tcacgtagag gtgatatgcc aacagaggtg tctcatctag ctaataatat
     3481 acaatacttt gaaggtataa attattttgt aaatattttg gtcgctcttg gtaaagaaac
     3541 gttccagaga ggatattcat atggtagcaa ttattcaaaa aaagatgttt taagttctct
     3601 acttaaaaat tgctatccta ctaaagaaga tgactcaaaa aaattaaaaa gtttacttaa
     3661 aaataccgac attactgagt taagattgat agaatctgct atgtatgccc ctcaatgggt
     3721 aaacataatt gaaaaatatt taaactggaa gggtctgaaa agtacaattt ggtacttcca
     3781 tgcacatata aatgaaggct tctctgcaca aaaagaatca gaggttgctc tattctcccc
     3841 tatttcacca cgtagtttca ttgatggtgc atttgatgta gattggtttt tagattcata
     3901 taaaactatt ggggaagaaa gattcaatat actttacaag tcagctaaat atataactag
     3961 tggtggtaac tctcatagac gtgcacaact ctatgctgat gctacacttg gtaagcttga
     4021 taaagacatg ttgatgaaag aaattgaaac aaatagaaat caagataaat tgagaagttt
     4081 ctctttactt cctatgaaaa aaggagacct aaaagaagca actttccgtt atgaatttat
     4141 tcaaaagttt gctaaagaaa gcaagcaatt tggtgcacaa cgtcgtgaaa gtgaatcaaa
     4201 agcttgtact atagcgctag aaaatctagc tagaagtgct ggatttggag atgtaaatcg
     4261 tatgatgtgg gctcttgaga ctgaaaaact aaatgaagtt gctcatctat ttaaaccaaa
     4321 agatattgat ggtacttcaa tttggataga tgttgatgaa aacggaaaag catctactaa
     4381 agttcaaaaa ggcaacaagt ctttaaagag tataccaaaa gctattgcta aacatccata
     4441 tgtacttgaa cttaaatctg tccaaaaaga tttaaagagt cagtattctc gtgcaaaaca
     4501 gagtttagaa caagctatga aaaatgaaat agtatttaca gttaaagaaa ttgccaatat
     4561 ctataaaaat ccagttatct gccctttgat taaaaatcta gtatgggtat gtgatgataa
     4621 tattggatat gtttataatg ataagtctaa gttctttatt tgcaatatag aaggtgaaac
     4681 ttataagttg acggcaaaaa ataaattaaa acttgcacat ccagttcatt tgtttgaatc
     4741 tggtaagtgg agtgagttcc aacatgattt atttagtaag caaattgttc aaccatttaa
     4801 gcaagtattc cgtgagtatt atagaataaa cagtgatgaa ttatctgaaa aaactatatc
     4861 tcgcagatat gctggtcacc aaattcagcc aaagaaaact gtagcacttc ttagaaatca
     4921 gctttggact gtcgattatg aagagggatt acagaaagta ttttataaag aaaatatagt
     4981 ggcaaagatg tttgcacttg cagattggtt ttcaccagca gatatagagc ctcctactat
     5041 tgaaacagta gaattttttg atagaaaaac ttataaaagt ttagaattag cttctatacc
     5101 tccaattcta ttctcagaag ttatgcgtga tatagacctt gttgtaagtg tcgctcatgc
     5161 tggtggtgtt gaccctgaaa ctagccattc tactgtagag atgcgtatag caattgctaa
     5221 agaactttta ttattattaa aaattaacaa tgtcaatttt gaaggctctc atgctcttat
     5281 agcaggtaat ttaggtgagt attctgtgca tatgggttct ggtattgtac ataaaaaagg
     5341 gatgggtgct ttgaatatac ttcctataca ttctcaaagt agaggtaaga tattccttcc
     5401 attcgctgat gaagacccta aaactgctga gattatgtct aagatactat tacttgctga
     5461 agatagtaag attaaagacc ctagtatctt aagtaattta taagtataaa aactatccct
     5521 aaaatatatt ggactttctt aaaaaaaatt agaaagtcca atatatacta tactgtaatt
     5581 tatatagatt aaatcataat tacaaataat ttttatataa acttctctta tacattatct
     5641 ttatggtact tattatatca cacaaaaagt atttttcttc tatcttcact agaatctttt
     5701 aaccctataa aatctatata aaaaatcttt ttctagagat ttgtttaata tggcattttc
     5761 ttgaaaattt ataaacctta tctctaatga agatttattt ataggtataa ccttatgtat
     5821 aaatcttaga tttactatgt attgtctatg ggtcataaag aaattaggag gtaggcgttt
     5881 ttttaaatca tcaaagttct cataaaactt tattatatct tcatctttag tgtgtagatt
     5941 aattgctctt gaatttttct caaacataac tatatcatca aaattaatca tatgtatctg
     6001 cttccttatt ttatatacaa aaagggtgtc ttctataaaa ttattattag caatcttatg
     6061 ggagttaata tggtctattg ctatgttaat tgaatcaagt attttttctt ttttaaatgg
     6121 tttaactaca aaatctatag gatgcacttt aaagctttca agactataat cagcgaaacc
     6181 cgtcacaaat actatatgta tatccttatc tatagaaaga atttcttcag cagaatcaat
     6241 cccattttta tatggcatat caatgtcaat aatcaataaa tctattttct cattttttat
     6301 aaattcaatt gcctctatac catccttaac ttttttaaca taatctacat atccagactc
     6361 tttagctata tttgctatat catctcttac aatcttatca tcatcacaaa tcaaaacttt
     6421 tatcatagac ttttctcctc ctaactaata atattttggg attaagatta caaattcagt
     6481 taaaaaatca tcactattta caagtatact ataatcatac ttatctaata tatttttaac
     6541 taaatgtagc ccatatcccc tatcttgtcc ttctttagta ctaaatccat tccaaaatat
     6601 actttcaaca ttatgtattt taggaccatt attttttata gctataatat actgaaattc
     6661 atcttcatat gcatgtactc ttatatactt cttttctttt tttatagtag acaaagactc
     6721 tattgcatta tctattatgt ttaatagtac tgaactcata tcattaggtt caatctctaa
     6781 attatccata aaatcttgaa tgtagacatc aaaagatata tccttttcaa gacattcctc
     6841 gtattttaat gaagctatag catctaaata agatactttt ccataccttg aattttttga
     6901 tttaaaacta tttgatatat tcattatata ttctttggca tcttcatcta gcccagtatt
     6961 taatagagaa tacaccactt gcaaatgttt caaatagtca tgtttttgac ttcttaattt
     7021 gataattata ttatccttat gatttatttt actatctagt ttttgtagtt tatattcctg
     7081 ttcattatta ttatttatca atcttataac tttaaagcct gtataaaaac ttaaaataat
     7141 taaaaccaaa aaaagtataa tatttaatac tccttcaagt aaaaaactat atcctaacct
     7201 taattgtata tatttagtgc cctccataaa taaaatcaat gcaaaacacg atataaaaaa
     7261 tgttaatatg cctcttttta atatgtctaa ttctttgtaa aatttatact tattcattat
     7321 aagattgcta aatataaaca aagttagtat tactatagga aatacaaaat tgttagccat
     7381 gatttccctc ccctctacaa tttatatagg gattatatca ttttaaaaaa attatgtaaa
     7441 aagttaaaat ttacatatag attaattaaa cttgtaaaaa tacagatgaa tttgtacaat
     7501 taactactta aaacttgaaa atacttactt tgctatatat tttagataaa tatataattg
     7561 atttagttaa gaaaggaagt ggtaaatatg aaagataatt tgaaaatagt actaaggtat
     7621 ataaaaagtt ataaagctag gtctttagca attgttgtga gtataattct tgctacatca
     7681 ctcgttgttg gtattggtac cctctcaaga agtgctcagc aagcagatgt agatagattg
     7741 aaaagagagc ttggaactga ccatgtatac tttaaaaata taaagaaaaa tcaattaaag
     7801 tccataaaat ctagcaaaga tattaaaaat ctgggtataa catcttacta tgcgtctaca
     7861 gatgttaatg aaaaattacc tataaacata ctatatgcaa ataaaaatta tttgactagt
     7921 cattctaaac ttataaaagg tcgatttcca aaagctagca atgaggtggt agtcgagaaa
     7981 tggattttaa atagtatggg cttaaaacca gagataaatc aaaaccttac ttttaaatta
     8041 tatcaaaaag aaaatccaga aacttttaaa gttgttggaa tccttgaaga tagatttata
     8101 gagaaaaaaa agggacaatg tgaaattttt tctaatctca atgaatcaga actgaatgat
     8161 tttacaactt atattgaatt taatgaaggc agtgatataa atacaaacat agacaatata
     8221 ataaaaaatg ctatgttaga tgagaaaagt ataggaagaa actcaatgct tatagaatct
     8281 actatgaaaa acggtacttt agataattca agtaaatacg ttgcaattgc tatgagtcta
     8341 tttgctggta ttgtaatata tagtatctat gttatatcaa tataccaaag agtacaggaa
     8401 tatggcgtct taagagctat tggttccacc aactttagga ttttcaaact tatgctttat
     8461 gaacttttca tacttgctct aatagcaatg cctataggta tatgtatagg tatgggaggt
     8521 gcacaaatat ttaataggtc tgtaggaaat attcaatttg aaggaaatat aaatgttaca
     8581 ccttttgtta ttccagataa aataattctc ttatcaattg catgtacaat actaataata
     8641 ctaattataa gtttctttac atacttaaaa ataagaagaa tttctcctat tgatgctatc
     8701 agaaaaactt ttggagctga taaaaatgta accaaagtta attatttaat atcaaaatta
     8761 actttaaata tgtcagtaac aaaatctata tctgcgaaaa acatctttag aaataaaaag
     8821 gggttcatta taattgtatt atctatgagt ataggaggta taatcgtcat aaaagacaat
     8881 tataaatatt cttttttaga tgtgcaaaac aaatctactc aagaacaaac atatatgaat
     8941 gcagatttta ttctatcaaa tgtttatctc aatgaaattg gtaacaatac ttcttttaaa
     9001 gatataaaag ggtttaatga taatcagata gataaaataa aaaatataga tggaattgac
     9061 aaagtcaaaa ctgctagtat cttaaacaca agaatagagg taaataaaat aaatagatta
     9121 gattactacg aagctataaa ttcaactcct tattataagc taaatcctct gttcataaaa
     9181 aataagacta ctggcaaata tacactaaag caaaagctaa aaggttataa tgatgagatg
     9241 ataaagtcat tagaaaaata cttagtaagt ggaaatataa acttgaaaaa aatgaaaaaa
     9301 gaaaatttag ctattgtata tgtaccagaa atttctaaaa cacagaaata cacaaagact
     9361 tttactcctg gtgaaggaac accagctgta gatataaaag taggtgatac tataaaagtc
     9421 aaatatccaa agggtgggat ggatacaggg ctttatgaaa gtctaaaaga taattatgaa
     9481 tacttagaat atgaatttaa agttggagct attataagtt atccttttgc agataatggt
     9541 atgtactctg gagatgatgg tgtagatgta ataacgagta gcgaatacct aaaaaaatta
     9601 actggtgtag ataagtataa cttagtttat gcagacctta atgaaaatgc aaacaagaaa
     9661 aaaataaata accttttagg taaaattgga agtgaagttc ctggaactac aacagtagat
     9721 atgatggtag aaaaagaaaa ttctgataag atgacatcta gaactatgat ttatagttat
     9781 ggtatagtag caataatgtt cataatcagt gtacttaaca taataaacaa cataagttat
     9841 aacttaactt caagaactag tgaatttgga atgttaagag ctattggtat ctctgagaga
     9901 ggctttaaaa atatgatact ttatgaagga atattatatg gtgtactctc aagtataatt
     9961 actatcgtgt caggaattat aatacaattt aaaatgtatt atatgcaggg ctttgtatca
    10021 tatggattgg gattttcaat agattacaaa atatatatcc tagttatttt agcaaatata
    10081 attgttggta ttctagctac atatattcca tcaagaaaga taaataaaat tagtatagtt
    10141 gaagctataa acattacaga ataggaggga taattatgcc tatattggaa actattaatt
    10201 taggtaagat ttatgggaaa aaggaaacat ctgtacatgc actaaagaat gcaaatttaa
    10261 aaataaataa aggcgaattt gttgctataa ttggaccaag tggaagtggg aaaagtacat
    10321 ttctacattt agtaggtggt cttgaaagac caagtaatgg tactataaag gtagatggta
    10381 aagatatatg ttgtctttca gataaagaat tagcaagata tagaagacaa aaggtaggat
    10441 ttgtctttca acagtataac ttaattcctg tattaaatgt aaaggaaaat atagaattac
    10501 cactaaagtt agacaataaa aaaatagata aagaatatat tgatgacctt ataaatttat
    10561 tgggtcttaa tgaaaggaaa aatcatcttc ccaaccaatt atctggtggt caacagcaaa
    10621 gagttgctat tgcacgtgct ttatctgcaa aaccaagtat aatcctagca gatgaaccaa
    10681 caggaaattt ggacagtaaa actacagaag aagtcatgga tttactaaaa agttctataa
    10741 aaaaatataa ccaaaccctt ataataataa cacataatga aaatattgct agaaaagcag
    10801 atagaattat atctataata gatggagagc ttaaattaac actctaaaac tatttataga
    10861 aaagaggatt taaatgttat tgattttagg taatttaaat ttaaacttgg attttgacta
    10921 cagaataata agagaagaaa atgatgatgt agatatattt atagatatta attatcgtag
    10981 cttagatata gatactgatg gttctaactt atttaattct agaatacaat ttccatttgt
    11041 aagagcattg attctaaggc taaataagaa caatcaatgt atgacaattc atttgttaag
    11101 agatatagat ttattctctg cttttgccaa ctttgaagtg gattatactg actctataat
    11161 aaatataaaa aatcaaaatg agaaagtaat tttaaataaa tctatcaaaa aatagtaagg
    11221 gggccttaat aggccccaat ttttttcttt tctaaactta ttatatcatg ataaataaat
    11281 tagtcaatag aattttctga atatttttat aattttttca taaagttttt tatatattat
    11341 gctttaaaat tatatgtaat aaaaaaattt tccgactaat tttaaagtta attgattaaa
    11401 tacacatatt cgaaattacc attaaattac tgacttttta tcatttccat ttagaattta
    11461 agttataatc tattatttat agttacttct acgtgtctct aataaaactc tatttatatt
    11521 caaatctaat aatttatata caaacccaat agaacctatg ttgcacaata gatattattt
    11581 accataataa acttttagat atatctcttt taattcactc atcaatgggt atctaggatt
    11641 agcacctgta cattggtcat caaaagcttg ttcaaccata gcatccaatc tttctaagaa
    11701 tttagattct tctactccag cctcttttat agtcttagga agacctactt ttgcctttag
    11761 ttcatctatt gcttttataa gattatctac tttttcttgg tctgtacttc ctttaatacc
    11821 tgcaaaagaa gcaaattcag catatctttg tatgcaatca ggatatctgt attgtgagaa
    11881 tgcacccatt ttatttggag cttctgcaca attgaacttg attacttcat ttattaataa
    11941 tgcatttgca acaccgtgtt gaacatggtg gaatgcacct aatttatgtg ctaaagagtg
    12001 acatattcct aagaaggcat ttgcaaaaga cattcctgcc atagtagatg ccattgccat
    12061 tttttctctt gcttctttat cattctttcc atttttatat gcacgaggta aatattcaaa
    12121 tattatttta cctgcttgta gtgctagacc gtcagctggt tcagttctta acatagatac
    12181 atatgcttct aatgcatgtg ttaaagcatc aacacctgat gctgctgtta atcttggtgg
    12241 catctccatc atcatatcag catcaattat agccatgttt ggcattaatt cataatctgc
    12301 taatgggtac tttacacctg aatcttggtc agttataact gcaaatggag ttacttctga
    12361 accagttcct gctgaagttg ggatagcagc aaaatacgct ttttcaccca tcttaggaaa
    12421 tacatatact ctttttctta tatccataaa tctcatagct aagtcttgga aatctacttc
    12481 tggatgctca tacattaccc acattatctt accagcatcc attgcacttc ctccacctac
    12541 agctattatt aaatctggtt ggaaatcagc catagcttta gctccttctc ttgcacaagc
    12601 taatgttggg tctggagcta catcaaagaa tgtagtatgc ttaattccca tctcatctaa
    12661 taaatcagtt aatttcttag tatatccatt attatatagg aaagaatctg ttactataaa
    12721 tgctttcttt ttatccataa catccttaaa ttctcttgct gctacaccta aacatccttt
    12781 tttgaaataa accttttctg gtgctctgaa ccaaagcata ttctctctcc tttcagctac
    12841 tgtcttgata ttaagcaaat gttttactcc aacgttttca gatactgagt ttccacccca
    12901 agaaccacaa ccaagagtaa gagacggtgc caatttaaag ttatataaat cacctatacc
    12961 accttgtgat gatggagtat taactaatac tctacaagtc ttcatagcat ttacaaactt
    13021 ttctactctc tctggatgat ttatagagtc tgcatataat gaagatgtat gaccatagcc
    13081 tccatctgca actaattttt ctgctttagc aactgcatct tcaaaacttt tagacttgta
    13141 cattgcaagt acaggagaaa gtttttcatg tgcaaaaggt tcatttaatt caacagattc
    13201 tacttcacca attattattc tagctgtttt tggaacttct acatccgcca ttttggctat
    13261 tacatgtgct ggttgtccaa ctattctggc atttaatgaa ccatcaatta ttactacttt
    13321 tcctactttt tcagtttctt ctttatttag gaaatgtgct ccacgatatt taaattcttt
    13381 tttaacttct tcatatattt tttctggtac tattacagct tgctcggaag cacatatcat
    13441 accattatcg aatgattttg atactaagat tgaacttaca gccattttta tatctgcact
    13501 ctcatctatt attgcaggta catttccagg cccaactccg acagctggtt tacctgaaga
    13561 gtatgcagat ttaaccattc caggtccacc agtagctaat ataaggtctg cctctgccat
    13621 gacaacatta gttaattcaa tcgaaggaac atctacccaa cctataagtc cttttggtgc
    13681 tcctgcctta actgctgcat catgtacaac tctagctgct tctatagtac agtttttagc
    13741 tcttggatga ggagagaaaa ttataccatt tctagttttt agtgctatta gggatttgaa
    13801 tattgcagtt gatgtaggat tagttgttgg tataactgct gataaaacac ctactggctc
    13861 agcaattctt gtaaaaccaa atgcttcatc tttttctatg acaccacaag ttttagtatc
    13921 tttataagca ttatagatat attctgaggc aaaatggttt tttataacct tatcttcaac
    13981 aatacccata ccagtctctt ccactgccat ttttgctaga ggtattcttt gttgattagc
    14041 tgcagttgaa gcagctaaga aaatcttatc tacctgttct tgcgtaaatg ttgcaaattc
    14101 tttttgtgcc aatctaattt cttctaatct cattcttaat gtttcaacac tgttgacaat
    14161 ttcaggacta catacttcaa catttgattt ttctactacc ttttcttttt tctccatact
    14221 aaaaacctcc ctaaaataat tatgcatctc taatttttat taatcttaaa tgcatcatct
    14281 atatttttaa cttatactta aaataatttt atagatttca tctctatacc
//
LOCUS       DACULQ010000101        14270 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.101, whole genome shotgun sequence.
ACCESSION   DACULQ010000101 DACULQ010000000
VERSION     DACULQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.101"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            174..407
                     /locus_tag="JA154_12000"
                     /pseudo
     CDS             174..407
                     /locus_tag="JA154_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417537.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(531..2291)
                     /locus_tag="JA154_12005"
     CDS             complement(531..2291)
                     /locus_tag="JA154_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAT7996573.1"
                     /translation="MQEVEVIKAKNVLARQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSKYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKTQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(2354..2497)
                     /locus_tag="JA154_12010"
     CDS             complement(2354..2497)
                     /locus_tag="JA154_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7996574.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            complement(2816..3055)
                     /locus_tag="JA154_12015"
     CDS             complement(2816..3055)
                     /locus_tag="JA154_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT7996575.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            complement(3060..3476)
                     /locus_tag="JA154_12020"
     CDS             complement(3060..3476)
                     /locus_tag="JA154_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAT7996576.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGAMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            complement(3473..3832)
                     /locus_tag="JA154_12025"
     CDS             complement(3473..3832)
                     /locus_tag="JA154_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7996577.1"
                     /translation="MPNVGQITGWSCFIGSRGSRMKHLHIQQLSTIGSSLKKHLNSTE
                     HNAPICGFLQHITGIICLAQDQYVCIALVLFYCIKLFQKIFAVGYQIPPFMVSVSAKR
                     GRSLFALATFAGKGVRL"
     gene            complement(4823..4993)
                     /locus_tag="JA154_12030"
     CDS             complement(4823..4993)
                     /locus_tag="JA154_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996578.1"
                     /translation="MMNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWS
                     MKPKKDTKESYK"
     gene            complement(5003..7768)
                     /locus_tag="JA154_12035"
     CDS             complement(5003..7768)
                     /locus_tag="JA154_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="P-type; involved in magnesium transport into the
                     cytoplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAT7996579.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            complement(7934..8245)
                     /locus_tag="JA154_12040"
     CDS             complement(7934..8245)
                     /locus_tag="JA154_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7996580.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            complement(8261..8491)
                     /locus_tag="JA154_12045"
     CDS             complement(8261..8491)
                     /locus_tag="JA154_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7996581.1"
                     /translation="MRREIEDLKRAILFAFYPVFTILCSYLGWNFFEQRMVIFKIWYQ
                     VSIVIWFILAVTIIVLFIKLLKLTKLRNRFKH"
     gene            complement(8492..9169)
                     /locus_tag="JA154_12050"
     CDS             complement(8492..9169)
                     /locus_tag="JA154_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAT7996582.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            complement(9307..9810)
                     /locus_tag="JA154_12055"
     CDS             complement(9307..9810)
                     /locus_tag="JA154_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix family transcriptional regulator"
                     /protein_id="HAT7996583.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            complement(9826..12489)
                     /locus_tag="JA154_12060"
     CDS             complement(9826..12489)
                     /locus_tag="JA154_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAT7996584.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGIYTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            complement(12582..12791)
                     /locus_tag="JA154_12065"
     CDS             complement(12582..12791)
                     /locus_tag="JA154_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996585.1"
                     /translation="MVKVKTKKKFVSTHMCQGEMPIYVRERYEKMLAVSIRAPNLEGM
                     VENFDKDLSRPNETDLIELKNAISK"
     gene            12741..13076
                     /locus_tag="JA154_12070"
     CDS             12741..13076
                     /locus_tag="JA154_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996586.1"
                     /translation="MTHMSADKLLLGFHLDHVSNLNELLRKVAHNNICIMSDRMTKVA
                     VWKPSVKTIQLVCMEYLNYIIDHSVIYEKIQCANWNGTEETNSIFNNSPSPLITLIYS
                     CDLQQNKTT"
     gene            complement(13275..14177)
                     /locus_tag="JA154_12075"
     CDS             complement(13275..14177)
                     /locus_tag="JA154_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT7996587.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIDARTQAISG
                     YLTKELQDLNMDTIRTDIPTSSAITDVIVWSIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDAGGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKESNWKIIG"
BASE COUNT         4392 a         3141 c         2338 g         4399 t
ORIGIN      
        1 aaagatgaac aaaaattata cagtttaata tggaatcagt aggtacatat gtattattaa
       61 gaattataaa taataaaaat aaattttagt caatatacaa aaacctttta aattgtctgg
      121 aaatcacctc ttcatttgtt ataaaaacat acaacaaatg aagaggtgat tttatggttt
      181 taaaaaaaat aattataagt ggatatgtgc tttcacatgt ttatcaacat ttgtaatgcc
      241 tatatttgct aatgaattat aaaaatatta aataatattc aactatagat tatgtaaatt
      301 actctatgaa aaaaatgagt tgtataaaat ctggctactg tagattgact atttctttta
      361 aaattacaat tataataagt tttatagaat taaaaataaa actttagaga tttttgataa
      421 cattgtagac taaatttgcc accgaaatcg ctttacagtg gcaaatcggt ggcaaataca
      481 atatattatt agtatataat ctatataaac attactattg tttgattatc ttagctacag
      541 tttccacatg tgtcgttact actatcggaa cacattttat caacctcgtt gctgtcatgt
      601 gaacacaatt cattagtcct atgccgtcct cttgcatttt tcctttgtgt tttaaattta
      661 gcaccatcta cgctattctt caagcctgtt ttataaacaa aggtcaactg tgcagggtca
      721 acatttccgt tttcatcaag tccacctaca attacttttt caacaatgct ttcaaacaca
      781 tagcggtcaa atttatcaag aacctcattc tgttccagag ttttcttaaa ctctttcaaa
      841 cgttttttta tgtctttctc attatcagaa gtttcttgta acttttgtct ttcttcaaca
      901 agctgttctt gtttacttac taaatcagca tatttacttt cgtaggtttc cttatcaata
      961 atttcttcca gacgcatatc cacaagttta ctttttttct tctctaatgc gtctatctcc
     1021 ttttccgctt ttgcaagttg ttttgacacc acactactac taagagtatc gtccattctc
     1081 tgcaaaaatt cttccagaac atctttatta tcatcacata ataaacgata actttctaca
     1141 aatgcttctt caatagctgt ttctggaata cctttgctat gtttacaaaa tttctttcct
     1201 ttcttcgtag ccgttacaca ttgccatata accttgctgt attttgagcc actgtgccaa
     1261 ttacgtcttg ttaaagtacc accacaaaag ccacattcta gcatacagct aaatgcgtat
     1321 ttcctgctat acttctcacg cttaccgctg cctaccgttg ttcggttctt atttctccga
     1381 ttaagaattt cctgtgcttt ttcaaaaact tcttcactaa taatcggctc atggtgatct
     1441 ttcatataaa atttatcttc ttcacccatg ttttcaaggc gacgtttgga aatcggatca
     1501 acagtaaaag ttttgcctaa gagtatatca cccttgtatt tctcattctt aataattcca
     1561 ataaccgtag atggtgccca tgtggaactc ccatacttcg ttttataacc tagactttca
     1621 agctcctgtc caataacaga accaccagca ccatcaacat aacggttgaa aatatatctg
     1681 acaatctctg cttcttcttg atttacagtt attgtcttat cctctgggtg ataatcatat
     1741 ccaatacaac cttgaaaacc taccagctcg ccgcgcttca ttttcatttt caaccctttt
     1801 tttacattgg ctgaaatgtt ctcgacttcc tgctgtgcaa cagaactaag aacaacaagc
     1861 aataactctc catccattgt aagtgtattg attttttcat cttcaaaata aacggcgata
     1921 tttctctctt ttagcatacg aacatatttc aatgtgtcta aagtatttct ggcaaatctt
     1981 gatatggatt ttgtaaatac catgtcaatt tcaccattca tacagtcatt tatcatacgc
     2041 tgaaaatctt ctcgttttgt tacttgtgtc cccgtaattg cttcatcagc ataaatgtca
     2101 gctaacaccc attccttgtt tttcttaatc atgtcagtat aatactgaac ttgtgattta
     2161 taactgttta actgatcttc gctatctgta cttacacggc aatatgcagc tacacgcaaa
     2221 cgctcaatgg cttttccacg ggcttgtctt gccaaaacat tttttgcttt tattacttca
     2281 acttcctgca tagtacacct ccttattttg tgttcctttc atattgttat tgtactactt
     2341 taaaaaacac aattcaagca gttatgtcag aaagcacctt atagtctttc atcagccttt
     2401 gttttacaag agtgtattct ttttcagaaa tcagtttacg ggcaagcagt tgttttaaca
     2461 tagcaatttg catactatac ctaattatat tattcattaa aaccctcctt gcttttttat
     2521 ttatggaagt aatcagacgg cttcggcagc tccactggac tttcaccatt cccattctga
     2581 tgggtgaacc gtgtcatacg ggtgtatcat tattcagata tacgagtcat ggcagcaacc
     2641 tttccaaaga tcgcttgcgg ttcactggca taatcgctcg ctgctttccc catttcagtg
     2701 gtcgtggcgt tccagtccgc tggctcgtcg tatatcaaac gtatctgatt actgtattca
     2761 cttgtcaaag aacggaaaag agttaaaatg aggtggtaga tactctctgt tatatttact
     2821 tcactttgac ttcaaaccct aagattactt taatcaacgc tgcccgtatc ctgcctttca
     2881 gttccatatc aagcacgata tacatattcc cgtgttcgtc atagaacggg cgtaggctgg
     2941 cttttgatat gtaagcgtca taatggttca aaatcttttc aattgccacc tcgttaccgt
     3001 cagctgcaga actgatggtc gagaatgatg gacacttttt tgtagacttc atcatgtttt
     3061 tattcctcct cgtacatatc cttaataagc tttaatgtgt gcagcctgtt tctacatacg
     3121 gaaaatcgtt ccatttccat aacctctgca atttcagcgt cggtcatttc aagaaagtag
     3181 gacataagca gaatgttacg tcggctttct gttaattctt tgatagcttc acataagcgt
     3241 tcatcataga tatggacttc tgaaccaaat acatcaaatg acgtaaactc aacgcaatat
     3301 tcgtccatgg caccgatctg gtttaactcc atttcagata aatcacagaa cggcgtttca
     3361 cgtcttgcac gtctgccaag ctctcggtca tagttcttta cagttcgtcc tacaaccttg
     3421 cgagctagac aatcaaattg aagtctgata gcgccctcga aagaagatgg tttcataatc
     3481 tcaccccctt tccagcgaaa gttgctaaag cgaaaagact tctacctctt ttcgcactaa
     3541 cactcaccat gaaaggtggg atttgatacc caaccgcaaa aattttttga aaaagtttta
     3601 tgcagtaaaa aagcacaagg gcaatacaga cgtactgatc ttgtgctaaa caaattattc
     3661 ctgttatatg ctgtaaaaaa ccacatatag gagcattatg ttccgtagaa tttaaatgtt
     3721 tttttaatga actaccgatg gtcgaaagtt gttgaatatg taagtgtttc atacggctac
     3781 ccctagagcc gataaaacaa gaccatccag taatttgtcc aacgttaggc aaaaataaaa
     3841 cggcggctat ataaccgtcg tcttattcaa aagttcaaaa agaactaaac taaaccctct
     3901 aaagcagtat gtatttataa tcagtcttgc aaaataacaa agtagaattg aaatataagg
     3961 tagcaatcct tgtgcatcta ataaacttat aggggttaca gcagaccacg gaataatatt
     4021 ttgtatactt tcagatcaat atagtccaac aaaaccaatt cccgatattt agtaactaaa
     4081 aagtaaacta gagtagaaaa catttctttg caaaacacaa ataataagta atggactcat
     4141 tgacaataga ttgagaaatc cagtttttta gacttagata gattatctca cttgtaaatg
     4201 atttaagttc tttgacttca tatatagcgg tataatctgt caacgtgatc attacaaacc
     4261 ataatgccta tataacatac tttccaagca aaaactttat tctattagtt ggggaataca
     4321 aaatatttat cattgtgcgt tcaacatatt ctttttgtat aaaaatgtaa ttacaaaaca
     4381 ccactcaata taagatagat aataatttga ataggaatta tcaatctacg taaccattca
     4441 ttacctgtca ttgttgttac aatagatttg ggattctgtt tccattacat aattttttta
     4501 tccttctttc agatatctcc tcattttatc gaagcttctt ctacagacat acattccata
     4561 ataaaatcta cctttgaata ttatttcacg atttcgttat agacgcgtaa acaaacaata
     4621 aaaaacgaaa tttcttttaa gtaccataat ttttatatcg aaattacaat tatccactta
     4681 tatacagcat ttataggtaa ttcaatgata aaaaaacaca tactttacta ctaaattgcc
     4741 acaattgaat gcgccttgat atgataggga gattccacaa taaaaaactg tgcatctccc
     4801 ttgattcatt catactgata tcttatttat aagactcttt tgtgtctttt tttggtttca
     4861 tggaccaaaa tactacattc attacaataa caatcagcaa cacaactgcc atagccaccc
     4921 aaaagccgat attaagacca agccattcgg tagttccaaa aagggtcatc caaagttctt
     4981 tccagttcat cattccacct ccttaaagca gttctccgaa atgacggaca taggcttttt
     5041 ttaaacttgt tgccaaagtc atatacagca gaatacatgg aatcagataa gcaaagtatg
     5101 ctgcaggcaa tgcaacaaat ccaagcatag ttccaaacac agtaaacgga ataatggtca
     5161 gaactataat tccagtcatt gttagcaaag tcaccggagc tgatgcacga ctctgaacaa
     5221 aaggtagctt tggagtacga atcatgtgaa tgactaatgt ctggctccac atagattcca
     5281 cgaaccaccc tgcttggaac atcccgatat actgtgcctg aagcattgcc agttctgcac
     5341 cgctgaaatg agcaggcaaa tcattaaata atacaccatt cgatacgaac aatggacaaa
     5401 atacaaagta cataaaaata taggttgtaa aatcaaagat agaactggtt ggtccaatcc
     5461 agatcataaa acttccaaca ctggaagcgt cccatttacg aggtttggta ataaactcct
     5521 catccacatt atcccaagga attgcagtac aggataaatc ataaattaag tttagaaata
     5581 taagatgaac actcatcatt ggtaagaaag gtagcaaagc agatgcagca agcactgaga
     5641 acatattgcc gaagttggat gaagcagtca ttttaatgta cttgatcatg ttggcatatg
     5701 tctttcgtcc ctcaataata ccctgttcca aaaccatcag atctttttcc aatagaataa
     5761 tgtcagcaga ttcttttgcc acgtccacgg cggtatccac cgaaatacca atatcggcgg
     5821 ctttcatagc tgcagcatca ttaataccgt cacccatgaa accgaccgta tgaccatttt
     5881 cacggagaac agaaaccaca cgggctttct gatcaggggt tagctttgca aatacatctg
     5941 tagactctgc cactcttgca agctctgcat cgcccatttg ttccaaatca gaaccgagaa
     6001 gcatattgcg aacctctaat cccacctgct tgcagatagt tcgagtcact ttgtcgttgt
     6061 cacctgtcaa aattttggtt ataacaccat ggtctttcag agctttaata gcatccgcag
     6121 tagattcttt tggaggatca aggaatgcca gatatccaat cagtaccata tcacactcat
     6181 ctttaacacc aaatgcgccg attggggaag gattactctt ctgtgcaata gctagtacgc
     6241 ggaatccctt atcgttaaga ttgtccactg tctccagaat tctgtaacga acgtcatcag
     6301 tcagaggctg cactcctcca ttacactctg caaacgcaca aatagagagc atttcttcca
     6361 cagcaccttt tgttaccatc tgggtcttac ccttattatc ctgtaccact gtagtcagac
     6421 gacgacgtgt gaagtcaaaa ggaatctcat ctactttcac atagttttca gacaggtcaa
     6481 tgagacgcga atctgcggct tcctcttcct ccgtcttatg gataattgcc aaatccatca
     6541 ggtttttata gccagtctgg aagtaactat tgaggtaagc gtgacgcagt actcgcgtat
     6601 cgtcctcgcc atttacattc agatggtatt ccagcacaac cttatcctgg gtcagcgtgc
     6661 cagtcttgtc agtacagaga atatcaatag caccgaagtt ctgaatggaa ttgaggtttt
     6721 taacgattgt ctgcttcttg ctcatggaaa cagcgccttt cgcaagacag gttgttacaa
     6781 tcatcggcaa catttctggt gtcaaaccaa cagcaataga aacgccgaat aagaaggccg
     6841 ccaaccaatc gcccttggtg ataccattga caaagaacac cagcggaacc ataatgagca
     6901 taaaacgaat caatacccaa gaaacggcat tgacaccctt ggtaaagctt gtttcaaccg
     6961 cttctccagc aactgcggac gccatagagc caaacagagt atgatcgcca acacaaacaa
     7021 caacagctgt tgcgctacca gaaattacat tactccccat aaaagcaatg tttgtgtaat
     7081 cagtcacgct ttctttctga gcacttacat tgggtgtttt ttcaataggt tcactctcac
     7141 cagtaagact tgcctgactg acaaacaaat ccttcgcatc caaaatacga acatccgccg
     7201 gaatcatatc tccagcagaa agatgcacaa tatctcccac caccaaatca tccataggaa
     7261 gttctttttt ttcatagttt ctacgagtaa ctgtacaagt ggtcgtaatc atggctaaca
     7321 atttctctgc tgcatttccg cttctggatt cctgtacaaa acgaagcgta ccggaaatga
     7381 ataccattgt taaaatgatt acaacagtca gggaatcaaa gtcctctggc acactgccaa
     7441 acagagaaaa atacggaaaa atcatatctg taattgtgga tactaaagcc agacaaaata
     7501 aaatggcggt aaatggattg ataaatgcgc caaccagtcg ttttgccaga gatttctttt
     7561 tttcatgagt gactttattg gtaccatatt tagcacgact tacggatact tcatctactt
     7621 ctaagccacg aagtgtggta tgaagttttt ttaaaacttc tttaatagga ttggttgccg
     7681 caaattgaat gcggcggttc tgttcgtcac ggatgactgc tttttctaca gtctgacgaa
     7741 tagccatacg attttctttt ttgttcattg gtcttacctc cttgaatttt cataggggca
     7801 aggaatatta tatgagcgac acggaatctc caaattctca tataatattc tctgccttcg
     7861 tggactgaca tcagagtcca tgtctttcac ctcacaatct taaaaaaata tctatgtgaa
     7921 acctcaatgt ggattattgg ttgcgatagc ggacactttt ttcgatatac cagacatcaa
     7981 agccggcaac aatcagccat gcgattattg caaatacaaa ccagtggttt gaaaatcccg
     8041 gcaaaaatcc tttcattaag aatataatcc catttaacgc aatcatttct ccaacattac
     8101 gacaaagcgg gacaatatta attttatctt tctcctcctg cggcatattt ttccacaggg
     8161 aaagatgacg atatcctttt ccaaaggcca accaaaagcc tgcaagagta aataaaattc
     8221 caaaaaagat acatgtaaaa tccatcgttt gttcctcctt tcaatgctta aacctgtttc
     8281 ttagttttgt cagcttcaga agtttaatga agagcacaat aatagtaaca gcaagaataa
     8341 accagatcac aatactcacc tgataccata tcttaaagat taccattctt tgttcaaaga
     8401 aattccaacc caaatagcta cataagattg taaatacagg gtagaaagca aaaagaattg
     8461 ctcgtttcaa atcttcgatt tctctcctca ctcacaacac ctcccaacca gcactaatca
     8521 cttccggaat taccaatacc tgcccaacga ttccttctaa aatattgttt ttggattttc
     8581 cgacagaaca gtattctgcg acaatctcaa ccttgtcacc aaccacatca ccgctttcca
     8641 gattgttcaa aaagagcgtc ttacaggaat tgctgttaat cagcaataag cgtatatcct
     8701 gctctgcctt ttcctgacat gtaaccgaaa tacgatactg cttttcagat tcatcactgg
     8761 aagtaattgg gtgaaacttt cttgcaaagg gacgtagaat caggttggaa ccaattaaaa
     8821 tagtagcggc ggtgatcgcc attgcataca ttcccgtact tgcaagaatt ccaatcgctg
     8881 cagtacacca taaggtggca gcggtattca ttccacgtac tgtccctctg tccttgaaaa
     8941 taacaccact gcataaaaaa ccaacaccgg atataatact gcttccgaca cggaatacct
     9001 gatccgaacc gtagagcata ggaaacaagg taaagaaact tgtacccatg cagattaaca
     9061 cattgatacg aatacctgca ggatgaccag tcaattgccg ttctaatcca ataagaaatc
     9121 caagtgttaa ggagagggca attctcaaaa taaaatctgt gtaaaacata tgaaacacct
     9181 ccctttaact actaacagta tataaaaaat ccaaatacaa tcctatggct aaagtcttgc
     9241 gattttcttg cgatttggta agaaaataga cggactgaaa ccgaaaatgg tcttagtccg
     9301 tctttgtcat atctgatcat taaatttgta tccaataccc cagatggtca aaatatatgt
     9361 gggacaatcc ggagcaggtt caatcttttt tcgcaacttc cggataaaag ccataatgtt
     9421 gctgttatcc agaatataat cttcctccca tacggcacga taaatctgtt cctttgtaaa
     9481 aacttcacct ttgttctgga caagaaagta caaaatatca aattcctttg gagtcaggtt
     9541 gacttcagaa ccttttatca gtacttttct gcctcttgga taaatttcca gatcccctgc
     9601 caggattcta tctgacagag tacccactgg ccaatcgctt tcttctgatg ctcctaacaa
     9661 taacgaagat acctctccac gaatcatctc ctgaatacca gaaagtaaat ccttcgcaca
     9721 atcactgtcc ggtggatctc ttagtagttt ttctaatagc cagatttcaa gagccgaatc
     9781 catcggaata taactaatat tctttttcat catgaatgcc tcctcttaaa tcaactcttg
     9841 atattttttc ttataaaaat atttggctac tgaattcagc agcatataga gagataccac
     9901 cagcagtaaa aatacgaaat accaaagagg taatttcgtc agtccaaaca acgacgcacc
     9961 gccgacaaat gtaagggaag taaaagcaat aatccccact agagttatag aaatgaccgg
    10021 tgcagatgcc ctgctctgga aaaaaggaat cttggaagtt cgcagaaaat gtaaaatcaa
    10081 aacctgtgtc cacatagatt ccaaaaacca tccagtctga aacagtgcta catactgaag
    10141 ttgcagggaa ggatctgtaa gctgcagata ggttgcccct ccacatagca tcggacatag
    10201 gaaataatac aaaaatagga aggtgacaat atcaaacaga gaactgatcg gtccgaaaga
    10261 cagcataaac tgtctcagtg ttttgccgga ccagtctctt ggggacagca tctcttcctc
    10321 atctacattg tcccaaggaa gcacaataca caacgtatca tacagcagat tcaaaagcag
    10381 aatctgaatt gaagtcatag gcaggaacgg taaaaaggca ctggcacaaa tgatggaaaa
    10441 aatatttcca aagttagagc tagcggtgat tttaatatat ttcagcatat tggtaaaagt
    10501 ttttcgcccc tccattacac cttgctccaa aacattcaag tccttttgca gcaaaaccac
    10561 atcggcagca tcttttgctg cgtccactgc cgtatctaca gagattccca catttgcttc
    10621 attcagtgcc ggaatgtcgt tgactccatc accaagaaat cccacggaat gaccattatc
    10681 ctgcatagca gagacaagac gcaccttatg tcccggagta agctctgcaa aaacatgagt
    10741 atgttccaca gcctttttaa gttcacagtc tgtcatttca tccagttgag taccagttaa
    10801 gatacgctct gtataaatac ccacccggcg gcaaacagac acagcaatcg ctgcctggtc
    10861 ccctgtcaga atcttaggga taacttttaa tcttttgagt gcagttacag actcctttgc
    10921 tgtctgcttt ggtgcatcaa agaaggatag atagccaact agagtcatgc cccattcgtc
    10981 atcaggagtt attttctttt gcgctcccac attttttcgt gccaccgcaa tgaccttcat
    11041 tccgtcctgt aacatttcac ctactacaga agatacactc tgtctcgcat cctgccccat
    11101 ggggagaagt tttccacgat attctacatg gttgcagcgt gagaatatat gttcaatatc
    11161 acctttcata ataagctgac tattcccatt gctatcctgt acaagtgtac tgacaaactt
    11221 acgagcatag tcaaagggaa tttcatcagc tttttggaat tttgtcagaa gatttgcgta
    11281 atgggcttcc cgccctggca tggtattgca ggcaagaatg gcattatcaa tggtattgca
    11341 gacaccagaa tgatagcagc tattcagata agcaagatca agaacctctg cattttcatt
    11401 cccaaaaata tccatataat attccagcag gatgctttca ttggtaagtg ttcctgtttt
    11461 atccatacaa agcacatcca tgctaccaaa gctctgcatg gcattaatat ccttgataat
    11521 ggtctgcttt ttgctcatag caagacttcc tttggcaagg caggctgtaa taaccatagg
    11581 aagcatttcc ggcatcagac ctacagcaac tgacagggcg aaagcaaaag attccatcca
    11641 ttttccatca gtaatcccca gtaatataaa tacaatggga actaatatcg ccataaaacg
    11701 tatcatcacc caggcgatgg aatcggctcc ttttttaaag gaatttttat catcagaatc
    11761 cggtttggta aatcctccat acagggtatc tttgcctact gccagtacaa tgccttcccc
    11821 ttttccactg atgactgtgg ttgccataaa agcgaggttt tcaagctgag tcagtgtatc
    11881 ctgctctccg taactgagtt ttcggcagct cttttcaatg atggcgctct cgccagtaat
    11941 cgcagcctga gagataaaga gatccgtaat cttcgtcaat ctgatatccg ccggaacacg
    12001 atcacctgca gagaatagta caatatcccc taccactaat tcttcgccag gaatctctat
    12061 taactttcca tcacgcctca ctgtgacgct ttcatgaact aggcgatcaa gctgagcagc
    12121 agcgctcttt gctcgcagct cctgaatcag acgaattacg ccactaatta cgatcatgga
    12181 aaagatgata atggcggtag tagcatttct cgcaaaattg gatgctacga aaacatccgt
    12241 aacaagagaa acaatgccca gaacaaaaag aatcacatga aacgggttaa taaatgcccg
    12301 tctcaaccgt tgcatcgtcg tatcattttt ccgcccattg aagctgttcg ctccgtattt
    12361 ctcccgcatt gaaccgattt gctcaatgga taaaccatcc ggagatgagc caatgtctcg
    12421 ataaatttct gatgttctac agtatgcgta ttttttgata cgactatcaa atagtgttgc
    12481 ttttgacacg aaacatcccc tcctttataa gtataagtaa taacatttta acacagtgtt
    12541 ctgaaaatgc tatatttata ttcttgcttt tttcttgcaa tttactttga tatggcattt
    12601 ttcagctcga tcaaatccgt ctcgttaggg cgagataaat ccttatcaaa gttttcaacc
    12661 attccttcta aatttggagc acggatggat acggcaagca ttttctcata gcgctctcgc
    12721 acataaatag gcatttcccc ttgacacata tgagtgctga caaacttctt cttggttttc
    12781 accttgacca tgtatcaaat ttaaatgaac tacttagaaa agttgctcat aataatattt
    12841 gtattatgag tgatagaatg acaaaagtgg cagtatggaa accgagtgta aaaacaatac
    12901 aattagtttg tatggaatat ctgaactaca taattgacca ctctgtaatt tatgaaaaaa
    12961 tacaatgcgc aaattggaac gggacagaag aaaccaatag tatttttaat aattcccctt
    13021 caccattgat tactcttatc tattcttgcg atttacaaca aaataaaaca acataaattt
    13081 taaatacgtt gactggcata attcatatgt atagtaagtc tacaattaag atgcgctttt
    13141 tctaatccaa atatattaag aaaaaagttt tgcgaagcca ttaatacttt gtttaagatg
    13201 tctttcataa atataaagaa taatgctctt aacaaaagcg tgcaaattcc taaagaaatg
    13261 cacgctttca ataattatcc tataatcttc caattgctct ccttatgtag cactaattca
    13321 aactgcgata cctgtgttgc ctttgtctga ttatcaatga atttcacggc aaccttgact
    13381 ttcacattgt ccccatcaac cgtaaagata gggtttacca attcagaata gaggtagtcc
    13441 ctgccgatag actcaagcac atttccagac acatagtaag caagctcctt ttctgtggct
    13501 gttgggtata gcttaaagaa agtttccaga aatgcagtag cgtcatttat ggtatccgcg
    13561 tctacacttg cgtctgcttc tggtgtcttt ggttcatagt ctgatttctc gattgctggt
    13621 gcaagggtag ggttctgaat aattaccata ttcccgccag cgtccacatg aacttttacg
    13681 gtataggttg ccttaaccgc tgttgtctgt tccccctctt ttatctgctg atctacttcg
    13741 taggtagcgg aaaaagtgtc cgttccagac tgttcaatgc tccatacaat cacatctgta
    13801 atggcagagc tggtcggtat atccgttcta atggtatcca tattcaagtc ctgcaattcc
    13861 tttgtcagat aaccgctgat tgcctgcgtc cttgcgtcaa tcgcttcttt gctgttactc
    13921 catgtgtaat aggactttgc aaaattcttc acgaaatttt ctatcccgtt ggtgtcctgc
    13981 aagcgaagct caatcacttc tttttcatgg gtcgtgtgct ggtcaatagc ggtaaaattc
    14041 ttatatactc caaagctcac gcttgcaata agcaccaccc acaatgcaat cacggttttc
    14101 ttatgtgtgc ctaccttgac agtacgtacc tttttttctt tgacattttc tgtctgtttc
    14161 ttattcttct taaacatatt tttcaagtcc tttctattgt tttactcgtc ctgcaccgat
    14221 taagtgctgt tgccagtaac tactacttag gtctgtatat cctatcgggt
//
LOCUS       DACULQ010000102        14177 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.102, whole genome shotgun sequence.
ACCESSION   DACULQ010000102 DACULQ010000000
VERSION     DACULQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.102"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            94..1722
                     /locus_tag="JA154_12080"
     CDS             94..1722
                     /locus_tag="JA154_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HAT7996588.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1843..2838
                     /locus_tag="JA154_12085"
     CDS             1843..2838
                     /locus_tag="JA154_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAT7996589.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3027..3410)
                     /locus_tag="JA154_12090"
                     /pseudo
     CDS             complement(3027..3410)
                     /locus_tag="JA154_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3636..3733
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3875..4232
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3875..3903
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(4518..4676)
                     /locus_tag="JA154_12095"
     CDS             complement(4518..4676)
                     /locus_tag="JA154_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996590.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5035..6405)
                     /locus_tag="JA154_12100"
     CDS             complement(5035..6405)
                     /locus_tag="JA154_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HAT7996591.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6805..7226
                     /locus_tag="JA154_12105"
                     /pseudo
     CDS             6805..7226
                     /locus_tag="JA154_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7604..7729
                     /locus_tag="JA154_12110"
     CDS             7604..7729
                     /locus_tag="JA154_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAT7996592.1"
                     /translation="MKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(8047..8715)
                     /locus_tag="JA154_12115"
     CDS             complement(8047..8715)
                     /locus_tag="JA154_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAT7996593.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9551..11971)
                     /gene="leuS"
                     /locus_tag="JA154_12120"
     CDS             complement(9551..11971)
                     /gene="leuS"
                     /locus_tag="JA154_12120"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089035.1"
                     /note="leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA
                     synthetase; charges leucine by linking carboxyl group to
                     alpha-phosphate of ATP and then transfers
                     aminoacyl-adenylate to its tRNA; due to the large number
                     of codons that tRNA(Leu) recognizes, the leucyl-tRNA
                     synthetase does not recognize the anticodon loop of the
                     tRNA, but instead recognition is dependent on a conserved
                     discriminator base A37 and a long arm; an editing domain
                     hydrolyzes misformed products; in Methanothermobacter
                     thermautotrophicus this enzyme associates with prolyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAT7996594.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(12325..12675)
                     /locus_tag="JA154_12125"
     CDS             complement(12325..12675)
                     /locus_tag="JA154_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAT7996595.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(12792..13364)
                     /locus_tag="JA154_12130"
     CDS             complement(12792..13364)
                     /locus_tag="JA154_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAT7996596.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(13365..14054)
                     /locus_tag="JA154_12135"
     CDS             complement(13365..14054)
                     /locus_tag="JA154_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinic acid mononucleotide
                     adenylyltransferase"
                     /protein_id="HAT7996597.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         4789 a         2296 c         1805 g         5287 t
ORIGIN      
        1 tataaaccta ttataattaa agtataatca tttttgcaac ttgtttattt tattataaaa
       61 atgtgaaact tattacataa ggaggaattt aacatgaaaa atcatactta taaagaaatc
      121 aaaaacatat atggaaaaat aagccctttt gaatttaagg acaaattaat agatattgca
      181 aaatacactg caaaagaaaa taacagagaa ttacttgatg caggtcgtgg aaatccaaac
      241 tggacttgtt caactgctag agaagccttt tttacttttg gtcatttcgc tattactgaa
      301 actcgttcta attgggattt aggtcatttg gctggaatgc ctcaaaaaaa gggtattaaa
      361 gaaagatttt ttaaatttat aaatgaaaat atagatatgc ctggagcata tttagctaga
      421 gatattataa actttggtat aaatgaactt ggatttgatg gagatgaatt tgttcatgaa
      481 ctagctgatg gaattattgg tgataactat ccacttccag atagaatgtt acctcatatg
      541 gaaaaaatag tacacgatta cctagttcaa gaaatgaaat atgatatatc aggtaaatat
      601 ggtgatgtag aaatttttgc tgttgaaggt gctacagctg ctatgtgcta tatatttgat
      661 tcattaatgg caaatgaact tctaaaaaaa ggagatacca tagccttaat gactccaata
      721 tttacacctt atcttgaaat acctaacctt cctcgttacg actttaaagt ggtaaatata
      781 aatgctaatg aagttgatga aaaaggtgct catacatggc aatatacaaa aaaagaatta
      841 gagaaacttc gtgacaaatc tattaaagct ttattcgttg ttaatcctaa taatcctgct
      901 tctatagcta tggatgaaac atcttgtaat aatctaatag atgtaattga aaattataat
      961 aaagatttaa tgataatatc agatgatgta tatggaactt ttgtagaaga tttttcatcc
     1021 ttaatgtcaa aactacctta caacactgtt ggagtatatt cttattcaaa atattttgga
     1081 gttacaggat ggagacttgg tacatttgcc cttcataaga aaaatgtttt tgataagaaa
     1141 ataaatgatt taactggaga attaaaaaaa tcagtagata agcgttacag tgatatgagt
     1201 cttaaccctt ctagtctttc ctttatggaa agagttgttg ctgatagcag acttgttgct
     1261 ctcaaccata ctgctggtct ttctactcca cagcaagtac aaatggcgtt tttctctgct
     1321 tttgcattga ttgacaaagt agatgcctac aaaaaactta atatgagtat ctgccataga
     1381 cgtcaaaaac tattatttga agctttagag cttccaatta atgaaaacaa aaacaatgct
     1441 gcatattaca ctcaatttga tattgaagaa tgggctaaat taaactacgg agaagactta
     1501 tttaaattta taagtaaaac tgcatctcca gtagatgttc tttataaatt agctaatgac
     1561 tattcagtag ttttacttag tggaaatgga ttctatggcc ctgaatggtc aattagaata
     1621 tctctagcaa atctatatga tgaagcttat actaagatag gaaaagccat aagagagata
     1681 ttaaatggtt atatcgttga atggcaaaaa attaagaaat aattatatta cactgaaata
     1741 tctaaattat atgaataaaa gagtttatgt agaccttaac tacttttttt gatataatcc
     1801 tatcggaatt ataaatttta tttatgaaag gcagagaata gtatgagtat taagaaaaaa
     1861 gttgccatag tttttactgg tggaactata tctatgacag tagatgataa agtcggagca
     1921 gctataccaa ctttatcagg tgaacaaata ctatcaatgg ttaccaacat tgataaagtt
     1981 gcagatgttg aagtttttaa ttttgatgaa attccaggac ctcacataac accttataga
     2041 atgatggaat tgaaaaatta tgtaaatgat ttattagcaa gaaaagatat tacaggtgtt
     2101 gtaataacac atggtacaga tagtttagag gaaacagctt attttctaga ccttacaatt
     2161 gacaacatca aacctgtaat agtcacaggt gctatgagaa gtagttctga acttggatat
     2221 gatggttcaa gtaatttatc tgcatcagtt tgtactgcag tatccaaaga tgctatggga
     2281 aaaggagtcc ttgtagtttt aaacaatgaa gtattattag cttctgaagc tactaaaaca
     2341 aatactcttt ctctaaatac atttaaatct ctaactagtg gcccattagg aataattgac
     2401 tgcaacgaac ttgtccttac aagagatatt gtaaatagaa caattataga tactgataag
     2461 gtagaatcta aagttgcctt atttaaagca tatgtaggag aaaatgctga ctttataaga
     2521 tttgctgttg atagtggtta taaaggtata gttattgagg caatgggcag aggtaatatt
     2581 cctcctcaaa tgctttctgg agttgaatat gctagagaaa aaggacttcc tgtagttata
     2641 gtttctagat gtcattcagg tagagtattc gatagttatg gttactttgg ttctggtaga
     2701 gatttaaaaa atcttggttg tatatttggt ggggacttac caggtcaaaa agctagaata
     2761 aaacttatct tagcacttgg aaaaactgat aaccttgatg aaatcaaaga cttctttgaa
     2821 aaaggaatct attgttaatt ctaataactt ctatttaatt atttattgat atttcaacat
     2881 ttttaaatca aaatctaata cttatgtcag ttaaatgtca gcaaaatatt ttatccacaa
     2941 tttttacaca aattttccac aacttatccc caatattatc cacaagaaaa aaggtagcac
     3001 ttatattagt agctaccttt tatttattaa tcttcatctt tccagctttt tatccaattt
     3061 cctatttcta ttattttttc ttcagttatt tcttctccat catgtaattt tgaaattagt
     3121 tctgataatg gattgttatg tagacctcca aaaattcttt ttccctctat tttgagatat
     3181 tctttctctt ttattaatac tctatagtgt acatgtttcc caattctctg cgatatgaaa
     3241 aaaccttttt tagttaatct agctaaaagt gtaattgtag ttgtatgctt ccaagcatat
     3301 gttttttcta tttaatctgc aacatctttt gatattacag ttttataacc agtattccat
     3361 atgtatttca taatttttaa ttctgcatct ggtaattttt tattcaacat aatatattcc
     3421 ttctttcaat ttatgtcact tcctcaaact tatctagctt aaattaataa taggagcttt
     3481 ttatcacaaa aactacaggt aaatttttct atatattcta cgtatttagt ctgtttttaa
     3541 gtgtgaaatt acgttcatac ctataaattt attcaatttt caaacaatct tgttgaataa
     3601 taactaattc tattgtatta tttagttata gaataaaact aaatcggcaa aactagagaa
     3661 atctagtgac gcaaagctat agggactaag acttatataa atatcttatg agttatgtta
     3721 gccagttgcc aaagagatat tgttcttttt gtttttatga aagtttttta gggggataat
     3781 aatttatatg tttagagata aaatggataa atgtacacat atattgactg cttatattag
     3841 caattcgtat gattattgta attttttgga tacaatttac attccatata gttaatataa
     3901 aactcaatta aattatgacc ttggtttaga tagttcatta tttacattcc atatagttaa
     3961 tataaaacca gaaggaatat ctggagttat tggagggtct attggattta cattccatat
     4021 agttaatata aaacatttac taagtgtaga tataattata aatacgattt aatttacatt
     4081 ccatatagtt aatataaaac ctgtagactt caaccctaca atgcttgttg gaagtgtatt
     4141 tacattccat atagttaata taaaactttt gtaaaagttt agaagtttat atataaaaaa
     4201 aatatttaca ttccatatag ttaatataaa accccaaaat aaattgagta tttccaatgc
     4261 ctacacatat acaaatctct caaatttgca gtgaaccatg agtagtgcaa ttgataacac
     4321 ttatcacaag tcctcaatgc cttatatttc aactgttaag ccttaatttc ttcaaaaaat
     4381 cgaacactgc aaaatttcta tgactttatt atatcataaa aatattattt ttgaatatct
     4441 gtaccaattt gtgatataat aaaagcaaga agaactacaa tctattttgc tgtagagtgg
     4501 agttcataat ttagaaatta cttagtttta tggaatctga tttttaaatc aaattcccaa
     4561 ccactcttag ttgccgcttt gagtggtttt ttacgctttc taaatagttt gctagctata
     4621 taaactatta agctagcaga tagacttgct agtatgcctt gtaaaaaatt atccatgtat
     4681 attcacctcc cttcttagcg tcgggaggat aattttttga tacatgaact ccactctata
     4741 aattgtagat tacatcttct tgctaaaaat attataacat ataattctta catattttac
     4801 ctatttatta ctttttacat tataataata ttcttttaat caaaaaaaca cctactatta
     4861 aagtaaatgt ttttaagtat ttttaatttt aaaatccata tagttcatat gaaactaaat
     4921 ataataactc aacatatgag caatatcgag acatttacat accacatagt taatataaaa
     4981 cgaaaataaa aaaattaaac ttataaaacc cttattaatt tacataccca ctagttattt
     5041 atattcaaaa aaatcttctc catctgggtt ataaaataat attctacttg ccaatccttg
     5101 ttcatattta taattttctt tatcacctac ataagaaaat gttaatgtca tgcttcttga
     5161 tggatctaat gaaattatat ctaaataaat agtacatttt tctcctggct ttaatacacc
     5221 gaaactatta aaatccattt ttattttagc atattgcccc atatataatg ttgaattagc
     5281 ttctccataa acttttaatt ttgttacatc taaattttgt gtacttgtat tttctatatt
     5341 tataggaact gttgggtctt tatatttttc agtataatat tcaaggttat catcataaca
     5401 ttcaagataa cttcctttat cttgccaaat tatgtgcttt gaatcaaaat caatctttaa
     5461 tccacatatt atatttttat ttgaagacgg aagactaata ttattgatgg tattttcaca
     5521 ctctagcata attttatcac ttagatttcc tatcccagtc acttttgcag catttttaag
     5581 tatatcttta ttacttccaa tatcaactag tacaatagga ttatttttag ccattggtga
     5641 agcaactaaa gcataaacta aatcagtacc actagctata taaaattctt ttgcgccatt
     5701 atagaactta tttactattt ttttattagt atcatatcta tctactccac ctaatctagt
     5761 tgaattagta tcattaacta gtttgtcact cattgatgaa gtaccaccta ttacataact
     5821 ttctacacca gttgtattaa atggaacact ttttccatca gttaatacta tagctgcttt
     5881 atctctaact gctacaggtg cagcactcat tgcatcaggc tctcctttaa aagcattagt
     5941 caatataact ttatttactt tgttaattga gtttatttct tttgcaacat tatagcttgt
     6001 cttaattcta tcactacctt gaagtctttt agcctctatt cctttatctt ttagaagagt
     6061 ttctgttgct ttatcaatag aactttctcc acctattatg tatacctttt tagctttctc
     6121 tactctttta agagttgcat taggtatgtt atttttctta gttaaaaaaa ttggtgcatt
     6181 tgttgctcct gcaagtccac tcgcacttaa tccatcagcc attgtgctgt ctgcattaat
     6241 taatatagct gtagtataat tttgttcatc agctataaga gcagccgttt catatttatc
     6301 ttttccttga atagtataaa ttgtatcaag tgcattagct gatataggac cagcagcaaa
     6361 cattgacacg gctaacccta gtgataataa tttctttaat ttcacaatat ctcccccttt
     6421 tatatatgaa atactatata tcttacataa ttctacatta aattttcttt tcctccttta
     6481 acgatattta tcttcctgtt aatttaagtg tatacaaata cgtgttataa tgtaactgta
     6541 aagaattaaa aagataatga cataattaga gcagtccaac tgttctttta taaaactaac
     6601 ttataaaggg agcgtacata tatgaatatc aaatcagctt ttataagaaa aagatgggaa
     6661 aaattctatg tatatgtgga ataagaaact ggcaaaaaga aacaaaaaag ttatggaagc
     6721 tataaaaaga aaaaggatgc tgaaaaacat ttaattgaaa taaagtctac tataaataat
     6781 aataagtttg ttgctccaaa aggtatgaca tttgtagaaa gatgttataa atatatagac
     6841 atgaataaaa ataattggtc tccatatact gttacaaata caaaatcttg ggttaaaaat
     6901 tacatagaac cttttttaag aatctaaaac tcacagaagt aaatcctagt tcagtacaag
     6961 cttttgtaaa ttataacttt aataattcta cctttactag tgctaaagta aggtatagct
     7021 ttctaagttc tgtaataagg gaagcgtata gattaagaga aatacaagaa aattcatgtg
     7081 attttataaa acttccttct aaagaaaaat attctacaaa tgaaatatac aatagagaag
     7141 aagtattatt gctaatagaa aagttacttg gtaataatat agaaatacct attcttttaa
     7201 tgttacttct aggtttaaga atgtgagaag ctattgggct taggtggtca gatgttgact
     7261 tagataacaa tctgataaat gttaatcaaa ttcttgtcta ttctaatggc aaaatatctt
     7321 ttaaagagcc aaaaacacca aaatcaaaaa gagctttatc agctccaaaa gaattaattg
     7381 aaaaattgaa aattgaaaaa ctaagacaaa gcaaattaaa attgtaaggt atcctggaaa
     7441 acagaaataa cttagtttgt ctaaacacca atttaaatcc gtttctacct tcttcactaa
     7501 gaaaaacgtt tcacaatttt acaaaaaaaa taatttaaaa aagattagaa tacatgattt
     7561 aagatataca agtgccactc tgctgctttt agggggaact aatatgaaag ttgtttctga
     7621 aagattgggt catacagata taaaaataac tatgaataga tattctcatg tacttaaaga
     7681 aatggacaaa gaagcttccg acaatctaag cgaacttcta tttaaatagt aaaaactgac
     7741 tgtcagttaa atgtcagtta aacctaaaaa aataggtttt gtcagttgat tatcagtcaa
     7801 ttattttata atagtttcac ttaaaaacgt ctatatctac acattttact atttgtttat
     7861 gcttattaat atctatctgt tattattaat tctaatagct tctatttaat tatttagttc
     7921 agtttacagg aaactacaca caaaccataa aataggatgt tgacaatata acagatagta
     7981 tgctcctgta aaaggtaaca tagtatctgt tatattgtct ggattcccaa attagctatt
     8041 tggaacttaa ctaaaaaatc ctaatataag ggcaactatt ataatggcaa tcggaataca
     8101 tactgtgtaa tcctgttcca tagagtcatc atctgaatgt agttttacaa ttcgcaattt
     8161 agcaatcagt ttgaagcaga aatagcctat tattgttcca attacgttcg tgagtaagtc
     8221 gtctatatcg gttgctctgt gtaacgtaaa aagctgaata gtttcgataa aaagagataa
     8281 tccaaaacca atcaagaatg tatttctcaa acgttcaaag gttttgctta taagcggaca
     8341 taaaaaacct aatgggacaa aaagtaaaat attcaaaata aaactcaaac taaatccatc
     8401 actaaaagga ataagattta tgtttggatt aaagaacgtt tcccctaact gtgacagtct
     8461 tatatattca ccgaatgtag gtataccaac aatatgcgat agtgttaaac ataagtagta
     8521 atacattgcc agtgataaaa tagatgtttt gacctttgga atatcactat ttttcttgaa
     8581 tattacaact aagaaaatac caatcaagat aattcccaac aagaaaatag gattaattaa
     8641 caataaatct ttgatagctt caaacagact gagttgaatt tctacacctg attgattatt
     8701 tgttacagtc tccatttgta catacctcct cataacattt caattatttc taaaaaacaa
     8761 aatagaagat aaatcttaaa atcatcctat caaaattttt aaatttgcct ttatcagttt
     8821 ataccaccag ttaattcctg tcttacttct gtatggtatt cttgggattc tttcatcaaa
     8881 ttagacaaaa aatggttacc atatcaataa actggttacg ctccatatct cccgaagcaa
     8941 ccatcccctg agaataaatc cgtatcagga atctccacat ctactgcacc tatcacaagt
     9001 tatttgataa catcctcaga caatcccaac ttggatatat cgacaacacc cattagaata
     9061 ccactgctga ccatacttgt tttcctgctg ataattttaa tcaactggga agttgaaacg
     9121 ctatcttttt tacaagttcc tttttagaaa catggtagct attatatgaa tctgaatttc
     9181 gttgtctggg tgtttcatat aaagcataga tatgcacctt ttttgcctgt tttcattata
     9241 tagaaaaaaa tttcaaaata caataattat atttttttcg tttaagctta gagcagttcc
     9301 ttgacgaaca acactttgga aactgaatga tttacatacc acttaataca ttaactcgat
     9361 ttaacattaa ttataataat atttaaaaca acaactaggg tatataagaa tgaacttttt
     9421 agttctttaa tcattttttt gtataaaatc aaatctattt ttataatttc aacaatgaaa
     9481 aaaggcttat ctctattttg agacagcctc atttatttta taaatttata tttttgttca
     9541 tatttttgag ttatttaaca actatattta ctaatttttt aggtacagct acaactttaa
     9601 ctatagtttt accttcaact aatcctttta ttttttcatc ttctaaagct accttttcca
     9661 tctcatcttt agatatattt gagttaacag ttaattttcc tctaactttt ccattaactt
     9721 gtacaacaac ttctatttca tcttttacta aagctttttc gtcatattta ggccagtcta
     9781 tatcaaatac acttccttct ttacctatca tagtccatag ttcttcacct aaatgagggg
     9841 caaatggtgc aattatagtt actatagttt gtacaccttc ttttataact gcttcgttta
     9901 tattatctaa ctctttatat ttatacatat catttattaa ttccataaga gctgatatag
     9961 cagtattaaa tccgaatttt tcacttaaat cagcagtaac ttttttaagt gtagagtgta
    10021 tagtatatct catatcctta tcttgggaat ttaattctcc aaactctaca tcctttttaa
    10081 ccacatctgc taactcgtca accaatctat aaaccctatt taagaatcta aagcatccat
    10141 caacaccttg ctctgaccag tccaaatctc tttctggtgg tgctgcaaat aatacgaaaa
    10201 gtcttgcagt atctgctcca tactcatcta ttatatccat aggagatact gtattccctt
    10261 tagacttact cattttagaa ccatccataa gtaccattcc ttgagtaagt aagtttttaa
    10321 atggctcatt gaagtctacc attcccatac tcttaaatgc ctttacaaac catcttgcat
    10381 aaagtaaatg cattatagca tgttctactc cacctatata ttggtctact ggatgccatc
    10441 tattaactaa ttccttacta aatggttcat tttcattatt gctatccaca tatcttaaga
    10501 aataccaaga agaatcaaca aatgtatcca tagtatcaac ttcccttcta gctggttttc
    10561 cacagtgagg acaagttgtt gacataaatt gctttgaagt cgtaagtgga gattcccctt
    10621 ttccagtaaa ttcaacatct gttggtaaaa gaactggtaa atcttccttt ttaactggaa
    10681 ctattccaca ttcatcacaa taaaccacag gtataggaca accccagtat ctttgtcttg
    10741 aaactaacca gtctctaagt ctataattaa ttgttctctt tcctctacct tctttttcta
    10801 acttatcgat tattccttca aatccttctg aagcatctaa tccattaaat tcttctgaat
    10861 ttatcatttt gttatcttct gttattacat caattatgtc taaattatat ttttcagcaa
    10921 aatctctatc tctttcatca tgtgctggaa ctgccattat agcacccgtt ccatactcaa
    10981 ctaatacata gttagctatc catagaggaa ctttcttgcc atttaaaggg tttataacat
    11041 atctacctat aaacattcct tctttttcta catcagctgc tgttctttct atttctgtca
    11101 tagtatgcat tttttgaaca aaagcttcaa catctgcttc atattcagtc cctgcaacca
    11161 attctttaac taattcatgc tctggagcaa gtaccatata agtaactcca taagttgtgt
    11221 ctggtctagt tgtaaatact gtcatagatt tatcagttcc atctatatca aaaactaaat
    11281 ctgctcctgt acttttacct atccaatttt tttgcattgt tttaactttt tctggccatc
    11341 catctaatgt atctaagtca ttaagtaatt cttctgcaaa ctcagtagtc ttaaaatacc
    11401 attgttctaa atctttcttt aatactgttg ctttacatct ctcacatgct ccttgaacaa
    11461 cttgctcatt tgcaagaaca gtctcacaag aaggacacca gttaacatat gattttttct
    11521 tatatgcaag cccatgctct aaaaacttca aaaatatttc ttgtgtaaat ctatagtatt
    11581 ctggagttga agttgcaact tctttatccc aatcataact aatccctaat aaatttaatt
    11641 gctctttcat ttcttctata ttttccattg tccatttatg cggatgtatt ccatgtttta
    11701 tagctgcatt ttcagctggt aatccaaaag aatcccatcc cattggatga agtacattat
    11761 atccttccat ttttttgaat cttgcgacaa catcacctat agaataattt ctaacatgtc
    11821 ccatgtgtat ttttccagat ggataaggga acatttctaa tgtgtaataa ttaggttttt
    11881 cagtttgtgc agtatccatt ttatactgat tattatcttt ccagattttt tgccatttcg
    11941 attctacttc tttaaaatta tatacgctca tattttgcct cctattttta attgtgtata
    12001 actaccttat ttagtattat ttacttataa tttccaaata attctttgaa aataaaaaat
    12061 cctcgtcact aaacttacgt ttagggacga ggataaatct tcgcggtacc accctaatta
    12121 gcctactaca tatatagact ctcttagata gcttttaatc cagccatgga gaatagattt
    12181 taatctagta agttcaaagt tatataatat tagttctcac cttccactaa ctctctgaaa
    12241 aaatataatc ttttactgct ctagtctatt caatttaata taatttttga aattttctaa
    12301 ttataataac atatattatc atgtttatgc taatgtgtca atatctatat aaggtgcatc
    12361 tttccataat ttttctaaat tataaaaccc tctattttcc tcattaaata catgaaccat
    12421 tatgtctcca taatcaagga gtacccaagt ttgagttcct tgtccttctt tacctcttgg
    12481 ctctaatcct agttttgcta attcatcttc cacattgtca gcaatagctt ttacctgtct
    12541 ctgagaagat gctgtagtaa taataaaata atcacacaaa ctagaaactt ttcctatatt
    12601 tattataact gtatcttgac ctagtttatc ttctatggca tcatatgcta tttttgtcat
    12661 ttgttccact gtcatataaa aattcctcac tttcctaatt aaaatacact tagttattgt
    12721 aacatattaa tacgattttg taacatagtc aaatttaaat ttattaatat gtttgccttt
    12781 atatagcttt tttataataa cttttcttta attagatagt ttcttgccat aactgttctt
    12841 ggatgtatct cttctttttt attgatgaca aacattaaag tattattaaa agaagttaaa
    12901 agtgcttcat caagcttccc tccatatgct aattctctta atgtatctac aactgggaat
    12961 tttcttcctt cttcaattaa atcagctatg taaattattt tttctaaaag agacatgtcc
    13021 tctctccctg ttgtatgata tttgattgca tttataatat cttcatcttc tacttcaaat
    13081 tcatactcgc atatgtacgc cccaattacg ctatgagaca aggaacgatt tccatcctct
    13141 attgggtcta aatatatttc atacttattt acataatcct ctatttcctt gtcacttaaa
    13201 tactttgcac aatcatgaat cattcctgct aagtaagctt tttctttgtc acatccataa
    13261 atctcacata atttttcagc acattttgca acattttttg aatggctaag cctccccact
    13321 ggtagcattt gatttagtct ttggttaata ttttctaagt tcatttaatc atctccatat
    13381 tgatatagtt tattttcata taaataattt tctacatttt cgggtactaa gtaacgtatt
    13441 gacttgcctt catttagctg ttcccttata tatgtggacg atatatccag tgatggaaca
    13501 taaactgtta taatgtcagc attatacttc ttagttagtt tttctatagt ctcttgtgac
    13561 cttaacaaac taattcctgg tctagttgcg gctataaaag tagctaactc aaagtttttc
    13621 tttacatctt tccactcttc aacactacaa attgcatctg cacctgttat aaaataaatg
    13681 tctgcatttt tgtatttttt ctttaagtat tttaatgtat ctattgtata agtccttttc
    13741 tttctttcga tttctacttt tgaaactaaa aaatcttcgt tgctttcagt tgctagaagt
    13801 gtcatattat atctatcata cttatctgta gtgatacata atttatgtgg tggatttcct
    13861 gatggtataa agattatttt gtcaatgtca tacttgtctc taataaattc tgctgttgct
    13921 aaatgagcat agtgtatagg gtcaaaagta cctcccaata tacctatttt gattttgcct
    13981 ttatggcgat taaatttctc tagtttttca gtatttttca actctgccat cttaactaga
    14041 ttttcacttc tcatattttg tctcctttta atgttataat actattgtac cattattttt
    14101 tatttcttaa agcttataat ttgttttttt gggtatataa ttatatttat atgctaaaaa
    14161 acacttaaaa aaataat
//
LOCUS       DACULQ010000103        13841 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.103, whole genome shotgun sequence.
ACCESSION   DACULQ010000103 DACULQ010000000
VERSION     DACULQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.103"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(127..969)
                     /locus_tag="JA154_12140"
     CDS             complement(127..969)
                     /locus_tag="JA154_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT7996598.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1221..3005)
                     /locus_tag="JA154_12145"
     CDS             complement(1221..3005)
                     /locus_tag="JA154_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAT7996599.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLVHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3071..3736)
                     /locus_tag="JA154_12150"
     CDS             complement(3071..3736)
                     /locus_tag="JA154_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAT7996600.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(3887..4102)
                     /locus_tag="JA154_12155"
     CDS             complement(3887..4102)
                     /locus_tag="JA154_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HAT7996601.1"
                     /translation="MFDKMYIKIYKFVIVVIIMANNELEALKNEIEAVREEINTYIEY
                     PEIFQEELVESSKKIDILINKYMYLSK"
     gene            complement(4126..4635)
                     /locus_tag="JA154_12160"
     CDS             complement(4126..4635)
                     /locus_tag="JA154_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase"
                     /protein_id="HAT7996602.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            4841..5059
                     /locus_tag="JA154_12165"
     CDS             4841..5059
                     /locus_tag="JA154_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAT7996603.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5077..7023
                     /locus_tag="JA154_12170"
     CDS             5077..7023
                     /locus_tag="JA154_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HAT7996604.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(7202..8257)
                     /locus_tag="JA154_12175"
     CDS             complement(7202..8257)
                     /locus_tag="JA154_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosugar isomerase"
                     /protein_id="HAT7996605.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8305..9111)
                     /locus_tag="JA154_12180"
     CDS             complement(8305..9111)
                     /locus_tag="JA154_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAT7996606.1"
                     /translation="MTLNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIK
                     KLYNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTA
                     LMGPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKY
                     GYQVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVIL
                     QDILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9098..9889)
                     /locus_tag="JA154_12185"
     CDS             complement(9098..9889)
                     /locus_tag="JA154_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IICD"
                     /protein_id="HAT7996607.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(9929..10351)
                     /locus_tag="JA154_12190"
     CDS             complement(9929..10351)
                     /locus_tag="JA154_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAT7996608.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10367..10846)
                     /locus_tag="JA154_12195"
     CDS             complement(10367..10846)
                     /locus_tag="JA154_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIB"
                     /protein_id="HAT7996609.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(10978..13734)
                     /locus_tag="JA154_12200"
     CDS             complement(10978..13734)
                     /locus_tag="JA154_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAT7996610.1"
                     /translation="MRDEILSFVNECVLFNSFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGIAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4463 a         2412 c         1590 g         5376 t
ORIGIN      
        1 cctattttat ctaaagatat ttatatttct acattcaata atagacaata ccctgcaagt
       61 taaagcatca tttttatgag cttatctcac agggtattat atgtttactt tatattataa
      121 ctcttgttat tgattaactt taataaatcc tttttctgaa acaagtgctt gtccttcatc
      181 acttaatata taatctatta aagctttacc tttttctgac aaaccatctt ctttattaac
      241 taatataaat ggtcttgata ttggatattt attagctatt acatttgcat cagttgcttc
      301 aacatcattt atttttaaac tatttacttt ctcatcaaca tatccaaatg atatatatcc
      361 tatagcattt tcatttcctt caactgtagt ttttatattt cctgaaccat cacttatttg
      421 tgcatcttta gttagttctt ctgaatcaaa ccctacaatt tcttggaatc catctctagt
      481 tcctgaacct tcttctcttg atactattac tattggtgca tcatttcctc ctacttcttt
      541 ccagttagta atctttcctg tatatatacc tttaacttgg tccattgtca gattttttac
      601 tgagtttgca gtattagtta tcaatgctat accatctatt gctattttag tttcctttaa
      661 tccagcactt ttttcttcat cttttaaatc tcttgaactc attcctattt cagatgttcc
      721 ttctatagca ttttttatac ctccagaaga accaatctgt tgtacttcta cactaacacc
      781 ttgatttttt tcttgaaact tttctccgat agtttccata agaggaccta ttgaagttga
      841 acctgaaaca gaaactttgc ttgaatctcc tgaatcacct gaacctcctg agttagaact
      901 acatcctact gctaaagctc caactaacat tgtactagct aaaagaacgc ctattttctt
      961 tttaaacatt ttacttaccc ccgcttatat tttaattttt tgttgtcatt tttctatatt
     1021 ttaataatac agattctttg ttaaatgaat attagagcag ggttaagtaa atgtaaatat
     1081 tgttaaatac atgtaaaaac ttaattttat atgatttatt cttaattaga ttaacttttt
     1141 aaagttactt taggcagata taaacataat acaactcata ttatcagaag tcttcattga
     1201 agctatcaac ctatcacatc ctataattct ttttctagtt tattaactaa ttcagcaaat
     1261 acatttaagg cttcctctat agattctttt ttcgtcatat ctacaccagc agattttaat
     1321 tgttctagtg ggtattctga ccctccactt tttaagaagt ttatatatct atctactgca
     1381 gtacttcctt ctgaaagtat ctgttgactt aaagcacttg cagcagaaaa ccctgtggca
     1441 tacttatata catagaagtt tgaataaaaa tgaggtattc tcgcccattc tattcctatt
     1501 tgtttatcta ctattgcaga ttttccgtaa tacttttcat ttaatttgta gtatatatta
     1561 gtaaattcat cagcagtaag tggttctcca ctctctaccc tttgatgtac aattttttca
     1621 aattcagcaa acatagtttg tctatgaact gtagttctaa attgttctaa ataataattt
     1681 aataagtata tagtttcatc cttagactta gaattttcta ataaataatt tataagaagt
     1741 aattcattta atgtagatgc aacttctgca acgaatatct tatagtccga atataaaaat
     1801 ggttgacttg ttcttgaata atgactgtga acagagtgcc ctaattcatg aactaaagtg
     1861 aatagagaat taaggtcatt tttatagctc attaatatat aaggatgtga atcataactt
     1921 ccccaagaat aagctcctcc ttttttacct tcattatcat atacatcaat ccacccttca
     1981 tcaaaagcac tttttatgac cttcaagtat tcttctccta aaggtttaag tgcctctaat
     2041 atgatatcct gtgctttatc atatggtata tccatgtcaa atttatctgt caaaggaaca
     2101 tacaaatcat acatgtgtat ttcattcaat cctaaaaatt tctttttaag ctcaacatat
     2161 ttatttagat taggaatact tttatctata gctgatataa gattattgta aacatctaaa
     2221 cttacatcat ctgaaaataa agacgcttct attgctgaat tatactttct agtttttgcg
     2281 taaaatatct ctgattttat tccaccataa agcgttgagg caaatgtatt tttatatttc
     2341 tcatatgttg aaaattcacc ttcaaaagcg tcttttctaa ctctactatt cttacttttt
     2401 ataaataaag agtagtttga atgagaaagc ttaaccttat tcccctcctc atcttctatc
     2461 tcaggaaatt ccatatctgc atatgctaac atatcgtata cattttctgg aattgtcgat
     2521 aattctgatg tagctgcaag tatttcttcc tctcgttcac tcaaagtatg tggtttatct
     2581 ctcaatatat cctttatata ttttgtataa aaggatagct tctcatcttt taagtattca
     2641 cttaacttat tctcttctat cgccagtatt tctggaacta tataagaagt agccacacct
     2701 aaatcagtag ataacatttc tgtcttagtg gctcttcctt gattatcatt aattctagta
     2761 tcttcatgtt gtttcatatg agtatataca tataaatttt gaacaatcct agatgcattt
     2821 tcagagatat ttaatacttt atataagttc tccttactct cagaaagctt ccctttatat
     2881 tcttttactt cttggataag attttttact ttacttatgt ctttttcaat ttcctcttta
     2941 ctggagtaca tcttatctat cttccatttg tacttttctt ctattgtact tctatcagtt
     3001 cccatatcat acacctctat tttaatttgt tttatatttt attcacttta caaaatatat
     3061 tgtatatatt ttagtccaaa taatctacat ttattactcc ttctatcata tttatttctt
     3121 tcataacatc gtgtaagtgt attctcttag cataagatac aaaaaaatta accttccagt
     3181 attcttcatt acgacattct atttccatat ttcttacgat aagttgattt ttttcaaatt
     3241 tatcatataa aaaaggtata gagtccaaat tgcttaattt taatatcaat acattctttt
     3301 cttggctaaa taaaaacgta ttgattatcc taagcacaaa taatgctatc attacaaata
     3361 atccacctag aattgccaat tcataataac catatccaac tgctatacca atacatgcag
     3421 ttgcccacat cccagcagct gtagtaagcc cttttacccc acctttattt tttaaaattg
     3481 ctcctgcacc taaaaaacca attccagata atacttgagc aggcattctt gctgggtcag
     3541 catttgtata caaattataa tcaaagaata attgaatcga agttattgaa acaatacaag
     3601 aacctattgc aattagtata tgtgtcctaa atcctgcaaa ctgtcttatt ttttctctct
     3661 ctaagcctat tattcctcct attattagtg ctaatagtaa ccttattgct atctccttac
     3721 ttcctatatc aatcataata taaaaccctc ctgacttaat tacaacttat gtcctacaga
     3781 taatttacac atcagaaata ctattttata tcttaatatt attattaata tatgtaaaat
     3841 atagccatct gttaattttt gatacaaaaa aacccacaac tgtgggctat ttgcttaggt
     3901 acatgtattt atttattaat atatctattt tcttacttga ttctactaac tcttcttgaa
     3961 atatttctgg atattctatg tatgtattta tctcttccct aactgcttca atctcatttt
     4021 ttaacgcttc taattcattg ttcgccataa taatcaccac tattacaaat ttgtatattt
     4081 ttatatacat tttatcaaac aatattatat tttgtcaata ttatgtcaat aaaacttact
     4141 ataaaaatgc tttaatttag gtattgtata cctggatact aagcctacaa acataccagt
     4201 tactaatgat gccattaaca taaatggtaa gtaccctata gcaagtatat tttctatgat
     4261 taaacttgca actattaatt gacctatatt atgccctatt gcacctataa tgcttatccc
     4321 tattagtgat aaatctttta ttttactcgc taaatacata aataaaagac tcagataagc
     4381 tcctcctata ctaaatagta gatatgataa agggcctgca aatattgatg acaaaattac
     4441 cctgatagta acaattataa cagtatcttt gacacctaac aacattaatg aaattaaagt
     4501 tataatattg ctcaaaccca attttgcccc tggaaatata actataagag gatttggtat
     4561 atatgtctct aatacatata aagctaaact ataccctatc ataagaccta aaaatgtcat
     4621 ttttctagtt ttcattaagt taactcctcc aaattttatc tatcatctta cattataata
     4681 caaaatatat aaaaacacct aatttagact aaaatatttt taatctattt agaataaata
     4741 ttagttattt caatgtgcaa ttacctctat aaatcttgac tttttgtatc atacttgtta
     4801 caattttaat gataatgaat ttcattagga ggtgttaata atgactgttt acgatttaaa
     4861 aataggagaa aaaggattta ttaatagtat agatggagat attaaattat ctaaaagatt
     4921 gcttgcactt ggatgtataa atagtactga aatagaagtt aaaaaggttg ccccacttgg
     4981 agacccaatt ataattagat ttagaggctt tgaccttgcg attagaaaat ctgatgctaa
     5041 aaatatttct ttacaataat agaggaggag ctacccgtga ttaatgtagc attattagga
     5101 aacccaaatg tcggtaaaac tactgttttt aatctactta caggttcaaa tcaatatgta
     5161 ggtaactggc ctggtgttac tatagaaaaa aaagaaggat ttcttggaaa agagataaag
     5221 gttgtcgatt tacctggtat atatgcaatg gatactttct caaatgaaga aaaggtttct
     5281 aaatcatatt tagaaaatga agatgttgat gtaatagtaa atgtagttga tgcttctaat
     5341 ttatcaagaa acctttactt aacaactcaa cttatgcaat ttaacaagcc tatagttata
     5401 ttgcttaata tgttagacat tgcagaatct aaaggagtta atatagatgc caaaaaatta
     5461 agtgaagaac ttggagtaat tgttgttcca attatagcaa aaaagaaaaa tggtgttgat
     5521 aggatagaag aagagattaa gggagctacg caaaaagctt ttatatatca gaaaaacttt
     5581 ggaagtgaaa cagaaacata taaaaaatta gaatccattt tatcgagatg tacaaaaact
     5641 acatatacac aaaagaaatc tatcagtgat aagatagaca atatagtctt gaatcctata
     5701 ttagcatatc cgatatttat tggtgtattg ttcctcttat ttaaatttac ctttgactgg
     5761 gtcggaggac cactacaaga gggatttgct agtcttatag aggcttatat ttctgcacct
     5821 gttagtgata tgttatctaa ctctagccca tggtttaaat cacttatagt tgatggaata
     5881 attggaggtg ttggtggtac tttaccattc ttccctttaa tatttacatt attctttggt
     5941 atatcgttgt ttgaagatag tggatacatg tcaagaacag catttttaat ggataaagtt
     6001 atgagaaagg ttggtctatc tggtaaagct tttataccta tagttatggg tatgggatgt
     6061 tcttctccag ctataatggc aactagaaca ttggaaagcg aaaaagatag aagagttaca
     6121 gctcttattg caccacttat gacttgtgga gcaaagctac ctatatatgc actatttgta
     6181 gctatattct ttccacataa tgctgcttta gtaactacat cactgtattt ggtgggtata
     6241 gttatggcaa ttgtagttgc attattctta aataaaacag cctttaaaac agaggctgaa
     6301 ccatttatat tagaattacc agaatataga atccctacta tagatgcact tattaaaaat
     6361 acttggaata aatcaaaagg tttcttgata agagttgtta ctgtcatgtt tgcaatgtca
     6421 gtcgtaattt ggggactttc ttcattcaat gtttttggat atacagaaga tataaatgta
     6481 agcttcttgg ctactctagg tcatattata tctcctatat tcaaacctct tggttttgat
     6541 gattggagaa cttcagttgc tattttaagt ggtcttggag caaaagaaat cgtagtaaac
     6601 tctttaaata tactatatgg taatctaact gttgtcttac caacaatatt taatggagtt
     6661 actgcatata cattcttgat atttactgct ttatatactc catgtatagc tgcacttgca
     6721 actatgaaaa aagaatatgg caataaaatg atgtttacat catttgcata tcaatttata
     6781 ctagcatgga taatggcttt tatagttaaa agtataggtg gagtactatt tatgggtaat
     6841 tcaataatgg aagctttgat aggtggaata attatagtcc ttgcactctt tattctgttt
     6901 aataaattca agtctacaaa aaacggaaat gtctgtagtg gatgttctat ttgttctgga
     6961 tgtccaagtg ctaattcttg ctcaagtaag gaagacagca ataaaaaaaa taatgcacac
     7021 taatatataa atattttagt ttaaaataag ataaaagtgg gtgtctcaaa attaactttt
     7081 tagttcatga ggcactcttt tttatataaa atcaaatata ttttcatcat ttcaacagta
     7141 aaaaagagct tatctagtaa aaaagaggct tatctctatt ttgagacaac ctcttttttt
     7201 gttacttatt cagtttcttg ctacctaact tcatgtggaa cctagtatct tttggaacac
     7261 ttgggtctac acctcttaat gtaggtattt ccccacatat tagttgtata ggtactataa
     7321 aattaaatgt ttgatagtaa ggattatctg tgatatcaat ctttaaattt ttatcatttt
     7381 ctgtaacatc tctacctata gcaaatacat tttccgtcca accagaaagt acatcaatca
     7441 tcttagttac tctaggttct tttcctgtat ctagtataaa tatagtagaa tcactattta
     7501 tagcattata tataccatgt ataaactctt caaactcata cccagttaca ggtattctca
     7561 ttgtttccaa taattttaat gctgcttcta atgtatctcc atatatatca gagtgaccta
     7621 ttattcttat ttctttagaa ttaactaact tttctttgtt tctttcaatc cattgtttag
     7681 agagcttata cactgcttca aatctattaa ttgcgtctaa tatcttattt atttcctcat
     7741 tgtacttttc actagatatt ataccttttt ctctagctat ttgaagacct aataacatta
     7801 agtttaactt tgtacaataa tatccttttg ttttagctcc agatttttct tcaccacaat
     7861 ttactgttaa tatataatca gaaatctcat ctattaaagc attcttacaa cctgccatag
     7921 atgctatttt acagccctta tcttcagcta acttcatagc gttgtatgta gaataactac
     7981 tcccaccttg agatacacct actactaggg tattttcatt atcaaacttg aatgtatctt
     8041 cagttatcat aaatggatac attttcacaa catctatatc taataaattt tgtaaatatg
     8101 gttgcacctg cactcctgag tgatagctag tacctgaacc tgttattata atctttttta
     8161 gatttgttct cttaacttca ttaaataagc tatctgcatt agatataatt tctctcattc
     8221 taactggtgt ttctaacata taatcttgta ttgtcatttt atacaccttc cttatctata
     8281 tattcaataa attataatta atttctataa caaacctata gcatgtaaag cgattcctgt
     8341 tactatagtg cctatagtta attttgttac acttaccttt ctctttaaca tgtagtatat
     8401 tacaaatgca aaggttaaag gtaacatatt aggtattatt ttatctagta tatcttgtaa
     8461 aattacttct gcaccattta agttgaattt cagaggagta gttatactca acatagttgc
     8521 aaccattcct cctacaacga ataaaccaac agttgtagcc atagacatta ttttctccat
     8581 aagtccctct ctttgtattc tttcaagaga atttacccct acttgataac catacttaag
     8641 tccaaatatt ctaagggcaa atgctggtat attgtataat agtaagaata atagtggtcc
     8701 aaagatatta ccttcttttg caagtgatac tcctactcct gctgctataa tcctaaatgt
     8761 tccccagaat aatgaatcac caattcctgc aagaggtccc ataagagctg ttttaacagc
     8821 actaattgaa ctttcatcaa attctggatt attagcattc tcttcttcca tagcggctgt
     8881 tatacctaat atagcaggaa ctaccactgt cgttgtatta aaaatctcca aatgcctttc
     8941 tattgctttt gcttgatttt ctttcttatt gtatagcttc tttattgcag gtaacattga
     9001 ataacagtaa cctaagtttt gcattctttc gtaattaaat gcaccttgaa gagcaaatga
     9061 acgccaaaac atacttctta attctttttt tgttaattta ttcaaagtca atctctcctt
     9121 ccgttaaagt attgattgct aattgaggat tttgattatt ttctttgtta ttgatataaa
     9181 agtttagtac aaatgctaca catactccaa ataaagctat agcagttata tctaatccac
     9241 cataagatgc taacaaaaac cctataaaga aaaatacaga catctttttt gaaagtaaca
     9301 tatccataag caatgcaaaa cctactgctg gcaacaatgt tcctgcaaca cccaatccgt
     9361 ctaatataac tttaggtatt gcatttatta atgctgtaac tttatcagca ccaatcataa
     9421 ttgctaaaaa ggttggaact gatgtactta aaaagaataa tattactggc aaccacatca
     9481 ttatggtaac tttcttaaaa tcagcctgtt tagcatagaa aactgccttt tgagaaaagt
     9541 acgaatttat tattctaact aatactccaa gagattgtgc taagactgct actggtactg
     9601 ctactgctaa agcaacttct gcaccattac ctgataatat agcaaaagct gtacctaata
     9661 cacctcctgt tactacatct ggaggagtag ctgcacctat tcctgcaatt cccatccaaa
     9721 ttaattcaag ctgtgcacca atcataattc ctgattttat gtctcctaaa actaatccta
     9781 ctaacatacc tgtaactatt ggcctatcaa gcatagattg accaaatata cgcccatcta
     9841 gtataccaac tcctgctata atagctaaca tagtagcttg tactaacata ttgaacctcc
     9901 attttaactt agtttaattt acattaaatt acattacatt tttgattttg ttttgatttt
     9961 ctaataactc atttacataa acaattccat tttttgcttc ctctaatcct gatttaacta
    10021 ttagttttgt atcctcctca actaataaaa gtatagataa aagcattggt aaatttactc
    10081 ctgtcaataa ttcaaatttg aacccttgta aaagtaagtt tgtacatatg tttgccacac
    10141 ttccaccaaa cacatctgtt aagacaacaa attcttcact atctttatta tttgataata
    10201 tacttctaat ttctttctca gcatcatctg ctgatttatc cgttgtcata caaaaatact
    10261 caatattagt taatgaacca attattaatt tacttgtgtc aacaagctct ctggacaatt
    10321 ctccatgtgt tgcaattaga aattttttca ttctctttct cctaaattat tttattaaat
    10381 ctgatagttg tttctttttg tctctaggta acattctaaa ttctatatca atccctaaat
    10441 cacctatttg tttaaagtta tttatatcat catcatcaac agcaactaaa tctgtaagtt
    10501 tcttcttccc ttcctcatat cttaatcctc ctacatttat ttcttttaat actcctcctg
    10561 aatttttagc tacaactaaa gcatctttgg tattttttac taacactaat acattgcttt
    10621 tctttgaaga agcaaatttt tctactattt ctccagattc ctctaccccc cttatatata
    10681 aagttacatt gctaggtttc gctaagttca gtgacatagc tttcatttgg tcattttttg
    10741 cttcatcatt tgcaactagt attacattta tacctagtga tgttgcccat ccatatgcaa
    10801 cctgaccatg tatcaatctg tcatcaattc tcatcaaaga taccattttc cccaaatcct
    10861 ccatcataca ttaaaatttt tattgtttat actctcattt atctataaaa gcaaaaagta
    10921 tgccaaatac tggcatactt ttttaaattt cattcactta ttttgtaatc ttgtatttta
    10981 ctgtggtaga ccatagataa tattataaat atatcctatc tctgaaattg gtatttgcac
    11041 actataactc ttttctagca cactaaatgc attttttatt agttttactt cactatgatg
    11101 gtttttgatt aacaaatcca tatcagtata ctcttcaatt tcagtttgtc taactaatct
    11161 ttcaatcata caacttaagt gaacatataa agaaatcttt ttatcatttg aaagtttttt
    11221 cttttgaata ttttctatag cattaattcc ttcttctatt ctctctatta tcttttttga
    11281 atctaaaata gttagactat ctattactct aattaatgaa aaattcttta caatagaatc
    11341 atttatgttc tttatttttt cattttctac tactctgcta aatattctaa aaacatcatc
    11401 ttctcctttt cctgatatta tatcctctaa taatataaaa tctaccccat tcacatttgg
    11461 attggcagtt cctactatag ccatcacatc atacatttca aaaactggtt ctttatctcc
    11521 catagaaaca agactatcat aatcataagg tattattttg acatcaacta ttccaattaa
    11581 actttcggaa agtagatgtt ggatttttat agcaatacct atccctgtaa aacaagatga
    11641 tattatagca tattctttat ttttttgagg ataatacaat tgtatattag gtttagtttc
    11701 ttcatccata atttctgcaa tctcttcaat gtttttacct tgcattaata aatttcctac
    11761 ttctagtgca gaaatagtgg atatttggtc tataaataac attgggctat ttatcttatt
    11821 ctttaaatct tcatatataa gattaagaga gcccatatca aataatacaa ttaaaccatt
    11881 ctttatccta tctccctcta gatatctaga catttgtttt gatacatcag caatagtagc
    11941 atctataggc atatcaatag ctgtaaatat atttttaccc aacatccgat tacatacatt
    12001 cgccatacta ctggcagtag catatccatg tgctataata gtcgcattaa cttgattaaa
    12061 tttcattctt ataattgttg atttaataaa tataatcatt atgattgtat ctacattatt
    12121 aagatacata tccaatctat tcgttattaa tgttgatatt ttacttatta tagagtattc
    12181 tttgtaactc tcttttttta aatatattag taatttattt tctaaagctt tatactcatc
    12241 agacaattga taagattcgt ccttcaaata aagataagac gaaaatgcca atacattatt
    12301 cccatcatat tttatattgt tattaacttc taaatttcta aatatatcct gcattatcga
    12361 tattataagc tccataagta cgttattatt ttcatcatta tttgaaaaga ctaaattgtc
    12421 cattagtcta atcattagat tagaactctt tttactaaat ttttcctttg ttatttcatt
    12481 tttttctagt ctatcaaagt aatctaaaca ttttatgaaa ctttgataga ttatatttct
    12541 tttatttctt ccatttgaca tttcaatact aaattgttta ggagaaaaga taatgctttt
    12601 gcttttacca ctttggaatt gaaatatatc tacattttct atataaagat ttctaggaag
    12661 gtcttgaaga gtaacaaaaa tatttccatt tcttctttga ttttttgcat aagctcgtcc
    12721 acatgcatat tttattacat ttttacattc acctatattc cccttgaatt ttatctttaa
    12781 aatcgcattt aatgtaattt gagttatctc tataggcaaa ccaagtacct tactttcttc
    12841 ataaataaaa tgttctacaa tctttctttt ttcaatagtc cctctttctt ctatattggg
    12901 aacataaata tttatgggta ttcttcttaa aaaggtttgt aaaaattcag atagactttc
    12961 tgttgtagca aatattaatc ttaccctagc ttttctaatt actccattct ctccaatcct
    13021 agaaaacgta cctgtatcca taaatgtaaa taacttttcc tgaccttcac tatttaatct
    13081 atggacttca tctaaaaata aaacaccacc atctgattgt tctattagac ctgctctaga
    13141 ttcatttgct ccagtatatg ctccttttac atgcccaaat aatagactag acaataactc
    13201 caaattatta taatactgcg cacagtttaa cacattgtat ggagaatttt tttctaatac
    13261 attactgctt acactatatt tgtatattat cttggctaaa aaagtttttc ctactccact
    13321 ttcacctgtc agcattattg taagtccgcc atttggataa agtacagcgg tttttatttg
    13381 ttcgattact tcttttaagc tattatcata tcctatgata ctattaaaca cagtctcttg
    13441 ctttaataat ttacactcat ttttaagttc atctaaattt tcataaatac ttttttttgt
    13501 aggtcctact acatcattta ttttattttt aggtaaaaaa ataacaggtc tactgtttat
    13561 ctttataaga ttatcagatt ttactaaatc atttaataat ctacttgatt gagttctact
    13621 taaattaaac ttttttacta tatcttctgt tattatgcca ctttcttttt taagttcatc
    13681 aaagctgtta aataatacac attcatttac aaaacttaaa atttcatccc tcatgttcaa
    13741 cctccaatac aaatatcaat aatactataa tattagtata tcatttatat atttatgaaa
    13801 aagataatta tattcctatc ttgattctct tttgtaaggt t
//
LOCUS       DACULQ010000104        13833 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.104, whole genome shotgun sequence.
ACCESSION   DACULQ010000104 DACULQ010000000
VERSION     DACULQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.104"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            376..3279
                     /locus_tag="JA154_12205"
     CDS             376..3279
                     /locus_tag="JA154_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter"
                     /protein_id="HAT7996611.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISVSEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3312..3758
                     /locus_tag="JA154_12210"
     CDS             3312..3758
                     /locus_tag="JA154_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT7996612.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3881..4183
                     /locus_tag="JA154_12215"
     CDS             3881..4183
                     /locus_tag="JA154_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAT7996613.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4364..5770
                     /locus_tag="JA154_12220"
     CDS             4364..5770
                     /locus_tag="JA154_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAT7996614.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            6114..7172
                     /locus_tag="JA154_12225"
     CDS             6114..7172
                     /locus_tag="JA154_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAT7996615.1"
                     /translation="MGDKMRASVLYSVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7271..7957
                     /gene="araD"
                     /locus_tag="JA154_12230"
     CDS             7271..7957
                     /gene="araD"
                     /locus_tag="JA154_12230"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088790.1"
                     /note="catalyzes the isomerization of L-ribulose
                     5-phosphate to D-xylulose 5-phosphate in the anaerobic
                     catabolism of L-ascorbate; links the arabinose metabolic
                     pathway to the pentose phosphate pathway and allows the
                     bacteria to use arabinose as an energy source; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAT7996616.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7975..8622
                     /locus_tag="JA154_12235"
     CDS             7975..8622
                     /locus_tag="JA154_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HAT7996617.1"
                     /translation="MLDSLKGKIVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8795..10162)
                     /locus_tag="JA154_12240"
     CDS             complement(8795..10162)
                     /locus_tag="JA154_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAT7996618.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10352..10440)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10868..12988
                     /locus_tag="JA154_12245"
     CDS             10868..12988
                     /locus_tag="JA154_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAT7996619.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKTIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKTCNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNILEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            13092..>13833
                     /locus_tag="JA154_12250"
     CDS             13092..>13833
                     /locus_tag="JA154_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HAT7996620.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSKMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDL"
BASE COUNT         5505 a         1583 c         2431 g         4314 t
ORIGIN      
        1 ctaaaatctt acctatataa gtatgtatac tatcatataa aaataaaatc ttaaaataga
       61 aagggttaaa aacacagttt attataattt ttattgttga gtagatacgt actattatat
      121 atgattgagc aagtttttca atcataaatt tataaagtat tttgcattaa aatttaaaca
      181 aagaattaaa ttatttaaga ttatgaataa ctaatacatt tagtaaatta ctaaatatac
      241 aaaatgtaaa tacttatcaa aatgtataaa tacttatgac atctagttac ttacagggtt
      301 taagtagaaa aaaatatcaa agtaagtatg gcatgatagt tgcttatata tttatcaaga
      361 aaggagttat taagcatgga gttgagagat aaacttgtaa acatcataaa tacagaaaat
      421 aaaaaagatc cattaacaga tgttcaaata gcaaagtttt taagtacaac tagagagaac
      481 ataactaatt tgagaaaaga acttaatata agtaattcaa ggcaaagaag atatccttat
      541 ttaaaatcag caatttcggc aatattacaa aagaataaga atatttctgt atcagaaatt
      601 acaagggaat tgatgacaga aggctttaat atatctagaa gagttgtgga ggaactttta
      661 cctaaagaaa gtttagaaat ggatgtagta gaggaatctg aagaagaatc gaatgaccca
      721 tttgaaactc tgataggtaa taaagggagt cttagaaatg ctgtagagca ggctaaatct
      781 gctatattgt atccaccaaa agggttacca acgttgatta ttggggaaag tggagttggt
      841 aagagtttat tttctagaca tatgtatgag tttgcaaagc aaaaaaagat agtaaaagaa
      901 agctccaatt ttgttgtatt taactgtgct gattatagtg acaatccaca attattatta
      961 tctttgttat ttggatataa aaaaggtgca tttacaggtg ctgaatatga tacaccaggt
     1021 cttgtagaag aagctgatga aggagtttta tttttggatg aaatacatag attgcctcca
     1081 aaaggccaag aaatactatt ttctatttta gataggggaa agtttaggag attaggtgaa
     1141 actaatgctg aaagaaaagt aagtattatg ttgattgggg caactactga aaatgtagag
     1201 actaatttat tattaacatt cagaagaagg ataccaatgc taataacttt gccaccatta
     1261 catgatagat tactaaaaga gaaaatagac cttatataca atatctttca acaagagtgt
     1321 aatagaataa atgcaaaaat atttgtagat aaaaatgtga ttgaaatact tgcactaaaa
     1381 aagtttagtg gaaatatagg acaattacaa aatatgattc aggttctttg tgcaagagct
     1441 tttatgaagt ttataaattc caaagatgaa gatagtgaat cgatagtagt agtagatata
     1501 aatgaagttt taaagcttaa agattctttt aaagatgctg cctttcaaga gattgaatat
     1561 acagagataa gaaaatattt gaaaaatgct atatttatac catttaattt agaagaatca
     1621 tcagtaaaag gaagtctttt aagtgatgaa tatatattac cagaggacat atacaaaaca
     1681 attgaaaaga aatattatga cctaaaatcc ctagacattg aagatataga tatagagaat
     1741 attttatgga cttttatttt aaatagattt gctgacttaa agtttaatct cgatagtaat
     1801 gaagaaattt tttcaatgag tgatttaaat agttttgtaa gtgatagttt agttaaactg
     1861 gtaaaagaac ttatgaatga tatagttgaa aaaaatatga atttagaagt aaataaaaat
     1921 atgttcaaat atctagcaat acacttagaa gaagctataa aaagaattaa gctaaatcaa
     1981 aagataataa atgtaaattt agaaaaaata aagattgact tttcaaaaga atatgaaatt
     2041 agtaaaagtt ttgcaataaa aattgaggaa tcaattggta ttaagattcc agatgatgaa
     2101 attggattta taacactcta cataaagtct gctctaaaaa atgaagtgaa aaaagataaa
     2161 gtagggttaa taataatttc tcatggacgt atagctacag agactgtaaa tgtagttaaa
     2221 gaacttttag gtgtaaagtt tcctgtggct atagatatgc ctttagatga aaaaccaata
     2281 aatatataca ataaggcagt agaactgtca aaaataatag accaagggaa aggcatattg
     2341 tttttggtgg atattggttc acttacaaac ataggtcaaa tagtaaataa gagaaccaat
     2401 ataagcacta aaactattga tagagtagac ctcctaatgg cactagaagc aactagaaaa
     2461 gtttctatgg gagaggaaga acttgatgaa atattttttt ctttgctaaa agataggatg
     2521 ggatataact atgaattaga aaaacacata gataaatcaa atgcaattgt aacattatgt
     2581 cttactggag aaggaactgc taagtatata agtaaaacgt tggaagaaaa gtatgataac
     2641 actaaatgtt atcaaatgag tgcattggat gaaaatttat tttgtaaaat tgaaaatcta
     2701 aaagagacaa acaatatatt agctattgtt gggacaataa accctaagat accaggtata
     2761 aattttatac cctataataa agaacttttc aaaaatttgg atatctatct atcaaaatca
     2821 aaacaacaag atgacagacc agttaaatcg tatgaaagaa tgcttgatga ggatttagtg
     2881 atttttgaac cagatatata ttttaaaaag gatttattag aatatgtatg ttcaattctt
     2941 ataaataaag aatatgtaga aaaagattac ttagattctg ttttacatag agaagaaatg
     3001 cttccaacat attctaaagg agctataggt gtgcctcatg gagattcttc tactgtaaat
     3061 agtacaaggt ttgtttttgt taaactaaag aatcctattg attggggtgt aggtaatgta
     3121 aatttcatat ttatgccagt ttttcaggct aatgataaag agattgttaa aaatgttctt
     3181 gagatactaa aagatgcaga atttatgaac aatgccaata agtgttttga taaagatgct
     3241 tttagaagga ttatatttga taaatttaaa catctgtagc catagcaaaa aaattgagag
     3301 gagttagagt aatgaatgta gatttaatat cattgggttt agaatctaat gattatgaaa
     3361 gtgttattga agaattagga agtataatgt gtaaaaaaga atatgtaaaa gaaacataca
     3421 taaatgctgt cttggaaaga gaaaggacat taccaacagg tctagatatt ggagaaatgt
     3481 gtgttgctat tccacataca gattcaaagc atgtaaatga gtctaatgta gctgtaggga
     3541 tattgaaaaa tcctgtaaag tttaattcaa tgatagaccc aaaagataga cttgatgtag
     3601 aacttgtatt tttattagca gtaaagaacc cagattcaca agtaaaactg ttaaaggatt
     3661 taatgtcagt ttttcaaaat ataaagttat taaaaaatat aaaaaacgca tcaacaaaag
     3721 aagaagtagc taaattattg gattttatag aaatttaatg taggagaatt ttataaataa
     3781 aaaattaaaa tttaaacaat caaagtaagt attatttttt taataggttt aatcttatat
     3841 aaaataaaaa tttagagtga aaaattagga ggaaattatt atgaaacaat ttaatgtatt
     3901 atcagtttgt ggttcaggga cagttacatc atctatggta gcaggtaaat taaaagaagt
     3961 actaggtaat aaggggattt ctattactac aacagaagct agaccaacag aagcactaaa
     4021 tttagcacaa tcaggaagat ttgactttat aacatttacg agtccacttc aaacaggcga
     4081 ttatggaata ccaactataa atgcatttgc ttgcctaact ggtatagggg aagaggcatt
     4141 ctttgaagaa gttttgactg ttattgaagg gatagataag taagttttgg agacgtgaat
     4201 ttatagtatt taaatcaatg atttagatac tataataagc taatattaac taaatatttg
     4261 tggaaaaaac attttatatg ttgtcataaa ttaaaaaatt ataaaaatag tcacttaagt
     4321 attaaattaa gcttataaat atttataagt aggagggaaa aagatgttag aatttatgaa
     4381 atctataatc gatacatttg gttctgcaat tatagttcca attataatat tcataatagc
     4441 aaagatattt aaagtaacta ctaagaaatc gtttctttct gctgtgtatg caggagttgc
     4501 tttacaaggt tttacactga ttttaaattc atttacacca ataataacac cagttatcaa
     4561 taggatggtt gagagtacag gagtaaacct accagttttt gatgttggtt ggcaagcaac
     4621 atcattggtt gcattttcaa caagtgcagg tatgatatat ttaggtcttg gtatattatt
     4681 acaaacaatt ttatttttga taaaatggac agacgtgttt caaccatcag atttatggaa
     4741 taactattct tatatggtat ggggagcaat ggttatagga gtaacaggaa attttccttt
     4801 aggtatagca tgtatggttt tattaaacct atatagttta ttaatatcag agttagttgc
     4861 aaagagatgg tctagttact atagatatcc taattgtaca ataatagcta tgcataatgt
     4921 tgaagcttct gtgtttgcag tatttgcaga ccctatatac aataaattgg gattaaataa
     4981 aataaaatta aatccaaaag aattagagaa gaaattaggg tttttaggtg aaccaattac
     5041 tttaggtctt tttttaggta tgtttatagg tatattaggt aatatgacga gaataaatac
     5101 tatggaagct tggggagaaa taatgaaagt aggtatatct acaagtgcag tgatggcaat
     5161 tttccctaaa gtagcaagta tgtttgctca agcatttgca cctataacag aagcagctag
     5221 aaagattatg caaaaagctg gaaaccgtga gtggtacata gcagtaaatg atgctgttgg
     5281 gtatggtgaa cctgcaactt taatatcagg tcttatatta attccaataa tgcttgtaat
     5341 agctatggta cttcctggta ataaggtatt accagttgtg gatttattgg caattccata
     5401 tatggttcaa ggtttagttg ccatacataa tggtaatata ccaaaagtat tggtttcagg
     5461 tataatttgg tttggattag gattatatgt ttgtacatct acagcaccat tatttacaga
     5521 tatggctaca aatataggtg tagctatacc tgcaggagca atgttaataa caagctttaa
     5581 tatactagga aaacctttga tgggattagt tttctttgca tttctaagtg caaatccaat
     5641 ttatataggt ctttcagttg taatttactt tgttctatgg gcattgttta gaaagaacaa
     5701 aacaagtatt cttgattatt tagaaaaaca agcacttaaa aatgtagaag aagagcctgt
     5761 agcagtttaa gtttataaaa atgaaataag aataattaaa ggagtttata attgatttta
     5821 tataagtatc agataatacc tgagaaatat ttaaatgaaa tatagacttc tttttatttg
     5881 ataatatttg taattataat gcattgtatc tttatagttg agttttctgt ataacctgct
     5941 atttaaaaca tactctttta tatttataca gtaatgtggt ttaattttat aaaatgtaat
     6001 ctctactggt aaaagacttt gtatataagg catttgcctg aataaaccat ttatcttttg
     6061 tatggcatag atattgctat atagaagtat atagcatgaa aggagatatt tatatgggag
     6121 ataaaatgag agcatcagta ttgtatagtg taggtgatgt cagatatgaa atggtagata
     6181 taccagaaat aacagatacg caagtgcttg taaatgtaaa atatgttggg atatgtggtt
     6241 cagatttacc taggtctatg gtaagtggtt tgagtggaaa tacaaaatat ccattgattt
     6301 taggacatga attttctggc gaagttgtta aaattggaga aaaagtaaag catattaatg
     6361 taggagatag agtagctgtt gcaccattag taccttgtgg aaaatgtgat tattgtaatg
     6421 aaggtaattt tggcttgtgt gatgattaca atataattgg aacaagagta aatggagcgt
     6481 ttgcagaata tgtaagagtt ccagaagaac atattttaaa actaccagat acattggatt
     6541 atgaaactgc tgccggaatt gaaccagcaa ctatagctta tcatgggatt tcaaagtcta
     6601 atattagagt aggagatagt gtagttgtgt taggatgtgg tccaataggt cagtttgtta
     6661 tacaatgggc aaaggtgttt ggtgcatcta agattatagc agttgatatt tttgatgaaa
     6721 aattagaact ttctaaactt ctaggagcaa actatatact taactcaaaa gaagtaaatg
     6781 taatcaaaga aataaaaaaa ataacaaatg gtggtgctga tgttgtaata gagactgctg
     6841 gtagccgatt tacacaagaa caatctctat ttgtagcaaa aaagagggga aatatagtgt
     6901 ttgtaggtat atcacacaca gaattaccac ttagtgcaga cgctactgag tgtatattac
     6961 gtggagaact taccttgaaa ggttcttgga attcatatac aagcccttat cctggaagag
     7021 catggactgc aacattggat tttatgggaa aaggtgacat tatttttaaa cccatgattt
     7081 cacataaaat aggtcttaat gaagtgggtg actttttaag taaaatgagc aagagagaaa
     7141 ttaatttcaa taaaatccta gtagaaatat aaaaatttaa taaaaaattc taaaagcaat
     7201 taaattttat aactatgaca ttaaataaaa taaaaataat tttcttaaat aaaatatagg
     7261 aggaagaaaa gtgttagaaa atttaaaaga agaagtatta aaagcaaact tagaattacc
     7321 taagaaaaag ctaattactt atacttgggg aaatgtaagt ggaatagata gagaaaaagg
     7381 attagtggtg ataaagccaa gtggtgtaga gtatagtaat atgactgtag aagatatggt
     7441 tgtagttgat ttagagggaa atatagttga tggcaaatta agaccttctt cagatacccc
     7501 gactcatcta gttttatata aagagtttga agaattgggg ggaatagtac atacacattc
     7561 aagttgggca acaatttggg ctcaaatagg aaaatctata ccatcttgtg gtactactca
     7621 tgcagattat ttttatggaa gtataccatg taccagaaaa atgactagag aagaaatttg
     7681 caatgaatac gaaaaggaaa ctggaaatgt aatcattgaa gaatttatgg gaagaaatcc
     7741 aattcattgt ccaggaataa ttgtaaatga ccacggacct tttacatggg gaaaagatgc
     7801 aaatgaggct gtacacaatg cagtagtttt agaagaagtc gcaaagatgg cttattatac
     7861 agagttaatg agtcctgata atattatgga taaggttctt atgaataagc atttttcaag
     7921 aaaacatgga aaaaatgcat actatggtca aaaatgatta tctggaggtg aattatgcta
     7981 gactctttaa aaggtaaaat tgtggaaata gcaattaaag cagagcatta tggtttatgt
     8041 aaagaaaaga ctggtaattt tagtattaga gataaggata ctggatatat acttataacc
     8101 ccatcaggtg taggaagaga agaattaaaa gttgaacata tttgtgtatt ggatttggat
     8161 ggaaatgtaa tagaagttga gaaaggtatt aagccaagta gtgaggtatt gatgcatatt
     8221 gaaatataca aaacaagaga aaatataaat gccatatcac atactcattc tttatatgca
     8281 acagcatttt cagtagcaaa gaaaccaatt ataccaattg tatatgaatc agttaattat
     8341 ggcggttatg tagatattgc tccatatgaa agaccaggaa ctttaaaatt agctaagagt
     8401 gtttcaaaat tactgataga tacagatgca tgcttattag aaagacatgg gttgacaact
     8461 gtaggtaatg atttagacga agcattatta aaatcaagat atgtggaaga ggtagcagaa
     8521 atatactata ggtctttagt attaaatcaa tttaaagaac ttgaagtagt agatgttgat
     8581 gagtttaaat cttggaaata cccagaagaa ataaagtttt aatctaaata attttcattg
     8641 aagtggttaa ttaaaatata tgtattattt atatcaatta ctatgtttta ttatgttaat
     8701 taccgtattt tgcaaatata tttattacat tttttcaaaa aaatatagaa tccgaaagcc
     8761 atataaaatt gacttgggat tctatacttt ttatttatgc atttttcttt atctgatttt
     8821 ctggagaatt tatatgttca aaatattttt tagtttcaga tactattata ggtgataatg
     8881 caagtaaagc tataaggtta ggtattgcca taagcccatt tacaatatca gcaattatcc
     8941 aaacaacttc taactttaag aaagcaccta ataacaccat agccacaaat acagttctat
     9001 acccattcat gccttttaca ccaaacagaa attcaaaaca tctttctcca taataactcc
     9061 atcccaaaat tgtagtaaat gcaaataatg ttaaactaac tgtaagtaat attggtccaa
     9121 atgtaggaat tgctccgtta aaagctgctt gtgtcataac agctccattt aggtctgaac
     9181 accacactcc tgaaataaca agagagaatc cagtaagagt acagattata atagtatcta
     9241 taaaagtacc tgtcatagat ataagacctt gttccgctgg ccattcagtt ttagcagcag
     9301 cagcagctat tggagcacta cctagaccag actcatttga aaatacacca cgagcgatac
     9361 catttctaat tgccagtgac atagttgctc ctaaaaaacc accagtagca gcagtaggtg
     9421 taaatgcact tttaattatt aacataaaag tttcagggat tttattccaa aaagatatta
     9481 aaatgattcc acagattaca acatatataa ctgccataaa aggtactatt ttagtagcta
     9541 cattagatat acttttaagt cctccaaata caaccaaagc tacaaatact gctaatacaa
     9601 cccctgtaat tctagggtct atcccgaaac tattatttat tgcatctgtt attgaattta
     9661 cttgagtaaa agtaccgatt cctaaaagtg caactagtat accacttata gcgaaaaata
     9721 ttgcaagagg tctccatttt tctcccatac catttactat gtaatacatt ggcccaccag
     9781 acacttggcc atttttatct ttagttctgt attttattgc aagaacacct tctgaatact
     9841 tagtagccat tccaaagaat gctgctatcc acatccaaaa aagtgcacca ggtccaccag
     9901 ctttgatagc tgtagcaaca ccaacaatat taccagtacc aactgtagca gctagagcag
     9961 tacataaggc tgcaaaactt gaaacatcac cactaccttt gttttcagca gagaatatta
    10021 acttaagagc tcttggcagt ttagttattt gaacaactcc aagcttaaca gtaagtagta
    10081 taccagtacc aactaatagt accagtaggg gtggtcccca aataaaagca tcaactttgt
    10141 ttaaaatatc aattagattc ataaaaacac tccctcattt tttttgcttt acataatcaa
    10201 tagaattttt aatgaagtaa ttgacaggaa ataagtataa tttattaata atttctagtg
    10261 taataaaaaa agctaaatct aaaaaagatt cagctctgga tgtacactaa ttagcaacca
    10321 aaataaaagt attagtaaat actgtttttg gtagataatc cctgtccttt tgcctgagag
    10381 attgagcata aaagccttgc cccttcggcg cccataatgg gtctctccag agttccgtaa
    10441 attttacagt cctactttta aaagtacctg agagtgttac tccttcggtg tgctatatag
    10501 caactcttct ataaaatctt aaccggatat tgatttgtca tagcttattg tattaataat
    10561 attaatataa gtttttatgt tttgtcaact aaaaaattaa aaaacaaaga aaaaatataa
    10621 aaatgtaagc ctatttacga attataaact tagaaagatg agtaatatac gtgaaaatga
    10681 attttgtaat acacatacaa atgaagtgta aaataagttc taaataaaaa aataataaaa
    10741 tcttaaataa ttaattagat atgattaaat tatggtaata ttatagttac gatattaaga
    10801 aaagttctat ttagttagat tttaagcgta atatgagatt atgtatagag aaaggatgta
    10861 tttttagatg ggaaaaatat tagtgctagc agaaaaacct tctgttggaa gagatatagc
    10921 aaaagtttta gggtcaaaaa atgaaaaaaa tggttatata gaggggccaa agtatgtgat
    10981 aacttgggca ctaggtcatt tggttacact tgctgacccc gaaagttatg gagaaagata
    11041 taaatcatgg agcttagaag acctgccaat acttcccaag caccttaaga ctgttgtaat
    11101 aaaaaagagt ggaaagcagt ttaatacagt taaatcgcaa atgaatagaa atgatataga
    11161 tgaaatagtt atagcaacag atgcaggtcg tgaaggagaa cttgtagcaa ggtggataat
    11221 agaaaaatca caggtaaaaa aaactataaa acgtctttgg atttcttcaa gtacagacaa
    11281 agcaataaaa gacggatttg caaaattaag aagtggaaaa gaatatgaaa atttgtatta
    11341 ctcagcaata gcacgagcag aagctgattg gattattgga ataaatgcaa ccagagcttt
    11401 gacaacaaag tacaatgcac agctttcttg tggaagagta caaactccta ctcttgcaat
    11461 gttgcttaaa agagaagaag aaataagaaa tttcaaacct aaagagtatt atggattaga
    11521 attaattgct acaaagggaa attcagatat aaaatttatt tggaatgata aaaacaataa
    11581 ttcttcaact tttagtaaag aaaagataga atccacatta aaaaaagtaa aaggtgtaga
    11641 cataaaaata tctgaagtaa acaagtcata taagaaaaaa tattcacctg cactctatga
    11701 tttaacagaa ttacaaagag atgcaaataa aatgtttgga ttttcagcaa aagaaacttt
    11761 atctataatg caaaaactgt atgaacatca taaagtactt acttatccta gaactgattc
    11821 tagatattta acatcagata ttgttggaac attaaaagat agaataaaag ctgtaaatat
    11881 tagtgattat tcaaagactt gtaataaatt attaaaaagc aatattatag ctaataaatc
    11941 gtttgttgac aattcaaagg taacagacca tcatgcaata ataccaacag aacagaaggt
    12001 attcatgagt gattttagtg acaaagagcg aaaaatatat aatcttgtag taaaaagatt
    12061 tttagcagta ctatctcctc cttttgaata tgagcagaca actataagtg caactgtaga
    12121 aggagaaata tttaaggcta agggaaacaa agtaataaat ttaggttgga aagaaagtta
    12181 taaagatatt gaagatgaag atgagtatga aagtatacca aatattttag aaaaagatgt
    12241 acttaaggtc aagggattaa agataacttc aggaaaaaca aatccaccac catacttaaa
    12301 tgagggaaca ttattgactg ctatggaaaa gaatggtcta gggacagttg ctacaagagc
    12361 agatataata gaaaaattat tcaattcatt tttaatggaa aagaaaaata aagatatata
    12421 tataacatca aaaggaagac aattattaga tttagttcca gtagacttaa agacaccaga
    12481 acttacagct tcatgggaaa agaaattatt ggatatttca aagggaaaac ttagtaaaaa
    12541 tacattcata aatgacatga aagattattc aaaagatatt gtactagaag taaaaaatag
    12601 tgataataaa tttaaacatg ataacttaac taaaaataga tgtccagaat gtggtaaatt
    12661 tatgttagag gtaaatggta aaaaaggtaa gatgttgatt tgtgaagata gagagtgtaa
    12721 tactagaaaa actatatcac aagtaaccaa ttcaagatgt cctaactgcc ataaaaaatt
    12781 agaacttcgt ggagaaggtg aggggagaat cttcacttgt agttgcggat acagagaaaa
    12841 gttgtcgtct ttcaacaaga gaaagcaaga agaaaaacag aaggcatcaa aaaaagatgt
    12901 taatcaatat ttaaaaaatc aaaataaaaa tcaagagaca tttaataatc catttgcaga
    12961 agcattagct aagctaaaaa acaaataatt ttaaagttat tattatacac aattataact
    13021 ttaactgtat attagttata tgtactgata taatatagtg tatagtttag gcatgaaagt
    13081 agggctgtta aatggattta agtggcatat ttaaatacta ttgtaaagag tgtgaaaata
    13141 catggaataa ttcgagtgtt gaattatttg agaatataga aacgtatagt aaagattcac
    13201 aaaaaaagag ggaaaaagaa ttagataaat tgctaaatac aatatcagtt catttagaga
    13261 ggtatccaag tgatgctgta ttgagaaaaa tgtgggtaaa aaagggcgag gttttcttac
    13321 aaaagacatt ggaaaaagaa aatattttta agttagaaaa aatggatgta gaggatagaa
    13381 aaaaattttt agatataaca aaacagttta ttagagatgc tagaaaattt gatgatgatt
    13441 tacctatagg tgatattatg caagctatga ggaatgtatg gatttcaaat gcattacaat
    13501 tattatttgg taaagaagta tattattcaa aagctaactt tgcatatagt atgttatatc
    13561 catatactga taattattta gacaatacaa atatagataa aaatgataag attttattta
    13621 ataactggtt agaaaaaagg ctcctgggag aacacattaa atctaaggat tatcatgaaa
    13681 gtaaagtatc taaaatgata gattatattg aaagtgtata ccctagagaa aagtttacag
    13741 aagtttatga atcgttatta ttaatattta aaagtcaagt aaatagttta aaacaacatg
    13801 gtaaggaaaa tcatttgtgt aaagaagatt tat
//
LOCUS       DACULQ010000105        13714 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.105, whole genome shotgun sequence.
ACCESSION   DACULQ010000105 DACULQ010000000
VERSION     DACULQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.105"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..255)
                     /locus_tag="JA154_12255"
     CDS             complement(<1..255)
                     /locus_tag="JA154_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996621.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDK"
     gene            581..838
                     /locus_tag="JA154_12260"
     CDS             581..838
                     /locus_tag="JA154_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996622.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            981..1607
                     /locus_tag="JA154_12265"
     CDS             981..1607
                     /locus_tag="JA154_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996623.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(1854..2633)
                     /locus_tag="JA154_12270"
     CDS             complement(1854..2633)
                     /locus_tag="JA154_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HAT7996624.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNRCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(2701..3126)
                     /locus_tag="JA154_12275"
     CDS             complement(2701..3126)
                     /locus_tag="JA154_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7996625.1"
                     /translation="MKDFEKLYEATTKLVSTSLKVKDDLKKMFKQNGYDITTDHYALL
                     RFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKDRRKFQIFLTDLG
                     RELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(3138..3815)
                     /locus_tag="JA154_12280"
     CDS             complement(3138..3815)
                     /locus_tag="JA154_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur flavoprotein"
                     /protein_id="HAT7996626.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            4048..4434
                     /locus_tag="JA154_12285"
     CDS             4048..4434
                     /locus_tag="JA154_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT7996627.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            4437..6236
                     /locus_tag="JA154_12290"
     CDS             4437..6236
                     /locus_tag="JA154_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HAT7996628.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSSNSVINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILKDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLNKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(6591..8465)
                     /locus_tag="JA154_12295"
     CDS             complement(6591..8465)
                     /locus_tag="JA154_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAT7996629.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(8470..10134)
                     /locus_tag="JA154_12300"
     CDS             complement(8470..10134)
                     /locus_tag="JA154_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucohydrolase"
                     /protein_id="HAT7996630.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(10148..10963)
                     /locus_tag="JA154_12305"
     CDS             complement(10148..10963)
                     /locus_tag="JA154_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAT7996631.1"
                     /translation="MYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICTGR
                     SMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAFSI
                     ESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKICLW
                     SNEKVFDEVKNILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKETIC
                     FGDGQNDVVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(11068..11832)
                     /locus_tag="JA154_12310"
     CDS             complement(11068..11832)
                     /locus_tag="JA154_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT7996632.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(11829..12578)
                     /locus_tag="JA154_12315"
     CDS             complement(11829..12578)
                     /locus_tag="JA154_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT7996633.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(12893..13075)
                     /locus_tag="JA154_12320"
                     /pseudo
     CDS             complement(12893..13075)
                     /locus_tag="JA154_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426285.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13082..13687)
                     /locus_tag="JA154_12325"
     CDS             complement(13082..13687)
                     /locus_tag="JA154_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT7996634.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
BASE COUNT         4861 a         2233 c         1473 g         5147 t
ORIGIN      
        1 tttatcactg gaaatctttt gtgaaaaagg cttgtttaac ttaatattat tgataactac
       61 atgtttaaat tcctctgaag tttcttctac tattacatat tttcgcttta aaaactctat
      121 tatttctaaa acagacttag aatttacttt taaaatactt ttatattttt catataaaga
      181 tttccactgt tcaatttttt cctgtggtac ttcttggata tgtgtactta ttttttcttg
      241 agatattttt ttcaattaaa ctctcctttc aatacttaaa tatccttata aatctcacat
      301 gactaaagtt acaaggttcc tacttaatag aattttgcat acttaaatat gtcttattaa
      361 ttctctacag gatatctccg tagttcctac gattcttaca taagttactt attttaaatt
      421 gttattagtt agattataaa aattatttat tttatcacta atataatact cttaaaaact
      481 ttacaaatca atttaaattt gctacctttt atttataaca acttatagta catttctcct
      541 atgcttttca atatattact tttaaaagga ggttttcatt atgattttaa atagagctga
      601 gcttttccga ggatattctt taaatagatt taacgagata tgcaatatat tatcagtaaa
      661 taaaattaaa tataagtata aagtgaaaaa taatgcttac tctaaaaaac ctttttttaa
      721 tgaaataaca cttggcaatt taggccaaaa ggaagatttc tcttatgagt acttaattta
      781 tgttcataaa gacgattatg aacaggcaga gtctctaata aattctaagt tgcgatagat
      841 tatctataaa acatgacatt ctgttgtcat gttttatatg ttattatact ttcaaatatg
      901 ctactatatt ttcaaaattg tgtgctttaa aactagcttt ttttagtaat ttaaagctta
      961 aaactaagga gactcgttat atgaaaataa atataaaatc aaagttaagt gaatttatta
     1021 aacagaacaa tctttttgat gacagcagaa taatatccta tcttcctaat ataacaatag
     1081 aaacagacaa aataaaaact ataatgatta atgaggttgt cccaactaac accaatgacg
     1141 atttttatag tgtagataaa gatgctgatt atttaaaaac tactattcct ttatttgaca
     1201 gtgcaggaat aaaagtatct aacataaatg atatcctaga tatgggtatc tatataacta
     1261 atgcagtaaa acttcctaaa tcagaatata caataactcg tgatacaatt aaattacata
     1321 tgcctatttt agaagaagaa attaaattat ttaaaaacct agaggtagta atgttaatgg
     1381 gagatgttgc aaaaaaatct tttaatatga ttactaaaaa gcatataaaa aagaatgtca
     1441 tcccttccat ttcaacatat aaacttcgta ataatgaact ttattatgat aatttaagag
     1501 tatttccatc atatattatg actggtggaa acatactgat tgaaaaatca aaatttgaaa
     1561 tggcatcaga agatataaaa aaaatgttcg agattataaa cggttgatat tacaaaataa
     1621 taaaaactaa tataaaataa aaagtgcctc aaaaacttct atttatgagg cacttttatt
     1681 ttatattagt gtaactatat actattaaca taatttatta tttatattgt tccttttaat
     1741 ctctattcca tttagtctag gtaacattac tctttacaag attactttat atttctttta
     1801 tatctctaac gtttttattt aataaaacaa ttattaatac aagtcaaata tatctattta
     1861 tctaaaaata tatcttttac agtaatatct ggatggtgat acttcttcct attcgttaaa
     1921 aaagattttt ttacattaat agcctctttt ggacaccagt gaaaacaagc taggcatctt
     1981 tcacactcat taccccactt tggcttttta tcaactatac ttatattctc tacaggacaa
     2041 acccttttac atgtcccaca tctattacat ttattttctg caaaaaattt agtagttgca
     2101 atttgcatat tcttacctac taaacgaagt acaacactat ctatatgtga ctttaaattg
     2161 ttttttcctt ctatatttcc aatctccaag ttattgatac attttgcaat ctcagctacc
     2221 ctattagtct gatttttcaa tctttcttct tcaacatctg gaggtgttat caaagcattt
     2281 ccaggcatat tgattaaaaa accagcagat aattttttcc cccttttttt aagtaactta
     2341 tctatagtaa ataaactatg tccagctacc ccattacaag tggcaatggc atatatatga
     2401 gtattaatat taaaatttaa tttctctaca aaagacttta ctatatcagg aacatcagca
     2461 aaataaacag gaaaaacaat taccaatata tcttcattta tgtctatatt attttctttt
     2521 ctatgtatag ctaagggtat aacttcttta cttccattta acgtttcttt taaaatattt
     2581 gaaactacta gcgaatttcc tgttccagaa aaataataaa tcttactatt cattcttatc
     2641 cctctttcta tatttctaca tcatcaatag ttaaaaagtc tcttaaaata ggatttatat
     2701 ttactttcca attctatcca aaatatcagt aaacaaaggc aattcttcat cagagataga
     2761 tttaaatgtc tctgttgcat atatacttgc aatttcattt aatggttctt ctaattctct
     2821 acctaaatct gttaggaata tttgaaactt acgtctatct ttaacatcta ctttacggac
     2881 aattaatccc ttatttttca taacatctaa gattcttgtc gtattagatt tatccttaca
     2941 tgatttttca cataaatcta tctgagaaat tccatcttgc tcccatagaa accttaataa
     3001 cgcataatgg tcagttgtta tatcatatcc attttgttta aacatctttt ttaaatcatc
     3061 ttttactttt aacgaagtac ttactaactt agttgtagct tcatatagtt tctcaaaatc
     3121 tttcatttta cctccattta aataatccct tgctcctccc atctatcaat tattccctta
     3181 tattgctcaa tatctttaaa agccattggc tttaaaacag catttatagc aataaaatta
     3241 tagatttgag aaagcacagg tggatttttc ttttcctcta tgcaataaat aaatttttta
     3301 gatacctttt gtatctttga ttctactttt tcagttatag gattttgatt aaatccagtt
     3361 tttatagata aactaccaca aacataacct cccgaatcta agaccatgga ttttataaaa
     3421 gaaattgctt ttgaaccacg tgcattttca tatgtagtaa ctgctataca tggcttacga
     3481 tacaataatc tttcaagcat catatgtgct ctatctgtaa acaccttgaa aagtccagtt
     3541 acactacttc cataagttgg gctaccaaat ataacaccat cactcttagt aattatatca
     3601 tgaatatact ctactttatc attttgattg atacacttgc ccattttata acaatttaaa
     3661 caaccaatac aatatgatgg attgacctca cttaaatcaa aataattaat ttttacatct
     3721 ggtattaatc tttctatatc tttatacaag taagtcaaaa cttttgatgt agctccattt
     3781 tttctagggc ttccatttat aactgtaatt atcatttcta tatctccttt ttagttgtta
     3841 tatcaacaat aagtatatca actatatttt tagctgtcaa tataaaccat ttatttaaag
     3901 ctatacttac acagcttaaa taaaagtttt atttaactac atgtgcttat ttaatcaact
     3961 ataaatgaat tatttttaat aattgacaaa ctaaattaat gataatatac tttatgtatt
     4021 acaaatgtaa gtagaaggag gtatagtatg aaaaagttac cacatatttc agaagcagaa
     4081 tatcaagtta tgaaaattat ttggaaatat gctcctatca gcactactga agtaattgaa
     4141 aagttggtag aaacaagtac atggagtcct aaaacaattc aaacaatgtt attgagatta
     4201 gttaaaaaag gagctttaac atatgaaaaa aatagcagag tattcgttta tactccactt
     4261 gttaaagaag aagaatacgt agctacagaa agtagttctt ttttaaatcg tttttataat
     4321 ggtactctta attctatggt actaaacttt ttagaaaatg ataaactatc tgaagatgat
     4381 attgaggaac tgagagaaat cttaaataaa aggacgacta agggggataa ataaatatgt
     4441 atgccccatt ctttatacga tttttactca gtacattaac cttatcaata ttaattgttg
     4501 taattttact tgccaaaaaa gtatttaaaa aacatatttc tgcaaagtac caatacaata
     4561 ttggatttat attgcttata atgttaataa ttccatttgt ccctcttaaa tatattaatc
     4621 ttggaaatgt atttaactat ttatatactt ttaatcaact tggaagcagt actaatagga
     4681 cttctatgat aaaaaatagt tcaaattctg taattaataa ctcaaatgca ttaaacgatt
     4741 tttcagtatc cctaaatcaa gttaattttg atagtttaaa tattttatta ctaatatcat
     4801 ggataattgg aattttaatc atgatagcta ttactattca ttgcaacatt aaaataaaaa
     4861 atatgaaaaa atcaatacaa attttaaaag atgaggagat agtatttata tttgaaaagt
     4921 gtaaaaaaga tattggtata aataaggatt ttatactatg caaatcacct attataaata
     4981 cacctattac atttggatac tttaaaccac acattatatt accagataag tctatatcta
     5041 aattatcttt aaaagatata aaatatatct tacttcatga attacaacat tttaaaaata
     5101 aagatatact gataaattat ataatgtgct ttcttcaaat attatattgg tttaatcctc
     5161 ttgtatggta tgcatttaaa gaaatgcgta tagaccgtga aattgcatgt gatatttcag
     5221 ttttaaaaaa gcttaataaa gatagtcata tagaatatgg acaaactcta ataaactttg
     5281 tggataatat ttcaagacca tcaaatctta cacttacatc agatattggt ggttcaaaaa
     5341 aacaaataaa aaagcgacta ttgaagataa tggattttca taatgaatca aatacgttaa
     5401 aattcaaaag cattttaatt tttacattga tagctttaat tgtatttgga aatattccca
     5461 aactatctat tatggcagat atcaacgaaa aatatagacc tacaaatgaa caaactgtgt
     5521 acgaggactt aagtcagtat tttaaaggtt ttgatggaag ttttgttatg tatgatttaa
     5581 aatccaagca atatcatatt tacaataaag aaaaaagtaa aacaagaatt tctccaaatt
     5641 ctacttataa gatttatagt gccttattgg gattggaaaa tgggataata acaagaaata
     5701 attcacatat cccttgggat ggaaagcatc attctaatag ttcttggaat gaagaccaag
     5761 acttattttc tgctatggaa aactccgtaa actggtactt tcagactcta gataaaaaaa
     5821 cagggctaaa aaacatacaa acttatctta ggcaaattgg atatggcaat tatgatgttt
     5881 caggtggtat ctcaaacttt tggctagaat cttcattaaa aatatcacct attgaacagg
     5941 tagaattact tgaaagtttt tatacaaata agtttaaatt tgaagataaa aatatagaaa
     6001 cagtaaaaga ttcattgtta atatcaaaaa aggatggggt atcacttttt ggtaaaacag
     6061 gcactgccaa tataaatgga aaagatgtaa atggttggtt tataggttat gtggaaaaaa
     6121 atgaaaatac ttatttcttt gctacaaata ttcaaagcaa agattattct aatggtagtt
     6181 cagcatctaa gataacatta tccatattaa atgacaaaaa aatctataat ccttaatata
     6241 tttactgtac attctaaaaa attcacataa ttttcatatt aattatcaat taaattcata
     6301 tacaaggctt attatataaa catagaagat agtaaaacta gcttaaaaac ataatataat
     6361 accctataca aatagtggca actctccctc acatcctcaa ttgttgccac tatttaattt
     6421 tttgtatgaa gttaaatata ttttcatcat ttcaacaatt tgggtgcaat acaatctcta
     6481 ttttgaaaca acctctttaa ttgttttaga gtttatgaat tatttctcat actttaaaaa
     6541 gattgaatta ctcttgataa tcttcttttg tagtcattac atatcaccta ctactcaaca
     6601 catattcttt gacctatttt tacatccttg tttatatcca aaatatttac cttcttacca
     6661 tctggaaaca ataccattgt caaagtagac tttcctaatt catgtattct ctttaaatca
     6721 acattaacaa gaacgtctcc ctgtttaacc atatctcctt gtttaacagc aacatcaaag
     6781 ccatctccat ttaatgcaac tgtatcaatt ccaacatgaa ttaatacttc tccattagtt
     6841 cctttaatcc caaatgcatg tccacttgaa accactgttt ctaatttacc tgaaatagga
     6901 gaaacaattt ttccatctat aatatcaata gcaaaaccat cacctaacat ttttttagaa
     6961 aatgtttcat ctggtacatt ttctaatgca acaattttcc ccttagctgg agacacaaag
     7021 ttttcattat tttcattaaa cactaattct tcgtcttcat cttcatttat agcttcttta
     7081 ggcactccaa ataaacatgt tacaataaag cagaatgcaa ccacaaatat tgatactaat
     7141 aagtatgtga ttaaattata tccatcatag atatacatta aataagaagg taaaactgct
     7201 aatccatatg aattagattg aatatgtaaa attgataata ttgctgctcc taaacctgaa
     7261 gatgcacaca taataatcaa aggctttaaa ttatatcgaa gctgaatccc aaataaaaca
     7321 ggctcactta taccaaaaag ttgagatagc attgctccaa ccgcagtttg tttaacttga
     7381 ttatttttag attttttcat aaatgctaga catgttccaa tattcgcaaa tccatacatt
     7441 gcacaaagag taataagggc attaaatcct gtatttgcta atagtgatgt ttcaatcatt
     7501 acataagtat gatgtaagcc agtaataaca gctaatggat atgtaaatcc aataataaat
     7561 cctccaattc ctaaaggcaa atcaatcaat aagctgatga tatcaaccat ttttaattct
     7621 attgtatgca ttatagggcc tacacctaac aaaataacta acattgttat tagcataaca
     7681 aaaaatggtg tgaaaataag gtctaataca ttaggcatat gtttacggaa ccatttttca
     7741 agattagctc caataaatgc tgtaacaata gctgttaaaa cacttccttg acaacctaca
     7801 ataggaataa atccaaatgc cataattgct tcaatttcat ttgatgggtc agcaacagaa
     7861 tatgcattag gaagtattgg ggaaactaac ataagtccaa taacaagtcc aataacaggt
     7921 gttcctccaa atactttaaa tgctgaccat acaataattg ctggtaaaaa agcaaatgct
     7981 gtttctgtaa gaaccgaaac aagtgtctga atatacaatg gaatatttgc agaagacatt
     8041 ccaaataatc caagtacatt atcattaaac aaacaaccct ttaatcccaa gaataatcct
     8101 gttgctgcaa taactggaat aataggaaca aatatatctg ctagagttct cataactttc
     8161 ttaaatccag tttcattatt tttaactaat tcatcttgtt ctttttttga taaagttttt
     8221 aatcccattt tttcaacttc tgcataaact ttattaacta ttcctgttcc taaaataatt
     8281 tgatactgtc cagaagtaaa gaaaacccct ttaacctcat caactttttc aactttctta
     8341 tcattaataa tttctctatc tttaaccatc actcttaatc ttgtggcaca atgtgtaata
     8401 gaaataatat tatcttcacc aataatttta ataagctcat ttgctatttt attatattta
     8461 ttcatttttc tacctcactt tcaataatac tgcatgaaat ggtttcaaac gcactgtttt
     8521 atttaataga tttatatcat ctaaattttg gaatacaata tcatattttt cttctactct
     8581 tatttctaca ggtttatcag aaaaattata ataacaatca ataactacca aatctaattg
     8641 ccttctatac gcaataatat catcactttc tatatcaata ggttctattg aaccataaat
     8701 caaacaatca gaatactcac tatcttgtct caactttatc atctttttat aaaattctaa
     8761 aatactctct gaattgttaa tctgactctg tgcattaata tctttataat tttcaataac
     8821 ctttaaccat ggtgttgtat ctgaaaaacc tgcattttct ttatctgccc atggaaatgg
     8881 cgttcttgca ttatctcggc ttcttaaatt cacaaattct aacgcttctt cttctgagta
     8941 actttcatct attgaacgat agtattggtc aatgctagat aaatcattaa aatcatttat
     9001 ggtatctctt ttaaaatttg tcattcctaa ttcctgtccc tgataaataa atggggttcc
     9061 tcttaaaaag aaatacattg ctcccaacat ttttactgca tcatcattcc tttcgtattg
     9121 caaaagatac tttgttgtag ctcttggttg gtcatgattt tcaataaaat tagctgccca
     9181 tccatgttcc tgaacataca tttgactatc taaaatgcat tttcttaaat ctggaattgt
     9241 ccatgaaatt cttttaaacc actcactacc agaagcaaca tctaagtcag catgtttaaa
     9301 gtcaaagacc attgaaaaga aaccatcttc tccaataaaa tcacctagct ggtcatagct
     9361 aatacctgct gcttcagcaa cagtcataca atcatatttt ttaaacgtat tttcttttaa
     9421 ttcataaagc attttttcta ttccaggctg atttctaact gtttttgtac atttaacaag
     9481 accatcaaca ccatcacttt ctaaactttg aaaacttaaa ttctttttga tgaatgtaat
     9541 agcatcaact ctaaatcctg ccactcccat ttgagaccag gtattaatca tttggtaaag
     9601 ttcacttctt aatattggat tttcccaatt aaggtcaggt tgttttttac caaaagaatg
     9661 aaaataatat tcgtctctat tctctacctt ttcccaaaca cttccaccaa aaacagacct
     9721 ccagttattt ggtaaatcgt ttcctttttt aaaaatataa taatcatgat aaggactttc
     9781 tggatttttt aatgcttctt gaaaccaagg atgttcatca cttgtatgat taagaactaa
     9841 atctaatata attttgattc ctctatcttt actttcttta attaactctt ttaaatcttc
     9901 catacttcca aattcacttg caatatccaa ataatcacta atatcatacc cattatcgtc
     9961 cataggtgat ttaaaaattg gacataacca caatgatgta atacctaaat tttttaaata
    10021 atctaaacga gaaataattc cctgtaaatc tccaattcca tcattattac tatcttgaaa
    10081 gcttcttgga taaatttgat aaacgatttc tttttgccac catttctttt ccataaatta
    10141 ctccttttta tatgatattt cttcttttta attctttata aattccatta tcgaatatgt
    10201 cctcacatat agatgtcgct atatctttaa gttgttgatg actatttttc attgcaattg
    10261 taacgtctga tgcttgaaac atgacaacat cgttttgacc atcaccaaaa catatggttt
    10321 ctttttgagt tactcctaat ctttcttgta atctcttaat tgctttgccc ttatgaaaat
    10381 ctttttgaat aatttcataa tactgactag aaatatctct ctgtgctaat tccattttat
    10441 cttgtagtat atttttcact tcgtcaaata ccttttcatt tgaccataaa caaattttat
    10501 gtatatcttg tgacttatac tcttcaatgt tattttcata tgttattttt tcttggatat
    10561 gttgtttatt tatacaagaa ttagttcctt tcaactgatt cattgtttca aatatttctt
    10621 tggctttttg attcatgaaa actttttcct gactctcaat cgaaaaagca acttctctct
    10681 tttttaataa acaaacaact tcctttatca atctttgatt aaaagattga ttataaagta
    10741 attcaccatg atattgaatg taattacctc ctcctgcaat atatccatca actcctaatg
    10801 ataaaacatc atcttgtatt gtacccatac ttcttccagt acaaataaca acactgcaat
    10861 gattcttttg acataaacgt atagcatgct ttgcagattc tgggattcca tatacctcat
    10921 ctctaagcgt accatctata tcaagtatta ataatttgta caatttcatc ttcctttctt
    10981 actatcaaaa tcataatagc attcaaaaaa aaagatatca atattttgat atcttttaaa
    11041 aaatatgttc atttttagca caaattttca ttttcgggga atatgttcac tttctaaata
    11101 catcttgtat ttaatataaa gcagttcaat taaaataaaa taaggtgttg ttacatcgta
    11161 gtccagttgt ttatctacag aaatagatat agattgaata tacaggtttt catcacataa
    11221 tcttgctaaa ggattactta tcatttttgt aatagaaacc atataaattc ctttcattct
    11281 catttcttta gcaaaatcaa taattgtaga tgcatctcca ttcaatgaaa taaagaaaat
    11341 catatcatct gtttttataa aatgattaag tgcctgaatc atatcataac cataaacatc
    11401 atatataatt ttccctgttg gtaagaaaat ccttttcatc tctttagcaa ctctttcctg
    11461 agaataacct tctccaaaga cgattatacg atttgccttt ttcattcttt caaacataat
    11521 cgaacaatcc ttcttctcta tatcatctat caccttatta taacaatcca ttaattggtg
    11581 tttactttga tttttatatt ctacttctgt ttcatttaag cgtataataa ctcttaattc
    11641 actatatcca ggtaattgta gtttttgagc aaaacgagaa agtgcacttg tagatatatg
    11701 gtatttgtct gcaaattcat ctatagaaag tttaatacaa tcttctttat gattaagaat
    11761 acactcacag atatacttat catttgctgt aaatgaatta taatatttat tgaaaagtcc
    11821 ttcaattttc attcttttct ccttctatac tctatgtaat tcatatgaag caaatctaat
    11881 agagcataaa atgctcctac gacttcataa gatatagctt gtaatgtatc aagtttttgc
    11941 gtttcaacat acagattata ctcacaaagt gttgcaattg tattattatc aagcctagtt
    12001 aaagaaatcg tatgtatatg attgttgata aattttgcaa tttcaattcc tgcttttgtt
    12061 tctccagaca aagaaatgat aataaataaa tcattttttt gaaatgattc tctcataaat
    12121 tcaatttcac ctatatcaaa taagtcaata acacatttac ctactgaaag aaatattctc
    12181 tttatttctt gagctaaact tttctgttca ttacctgtcc cataaatata aattgtttct
    12241 gcatcatata ttattttaca aatatcttga aatgatattt tttgtaattc attcattaat
    12301 agtcgataat tctcgcaaac atcatgaaaa ttattatcat tattaacaaa ttctagttga
    12361 ttatttttaa gtaaatactt taaatcagag aatgaatcta atccaatttt tctacaaagc
    12421 cttaataaag tagctctaga aacattacat tcatttgcaa ttttttcaca cgaaaaatta
    12481 cggtattctc tttgacaaat caatatttgt ttaataataa ttttatcatt ttcatttaaa
    12541 ttatcataat tttggttaac taacgattct aatctcattt tttccactcc ctaaatatta
    12601 ttttaatata cattagttgc tttttctagg gaagagtttt aaataataag gataaaataa
    12661 tcatttgatt ttatttttat taaaataaaa ataacctgct aaaagcaggc accaaaaagt
    12721 acttatcctt tcgaacatgt actttagata ttgctttctt cggaagcttc atctcttcat
    12781 actctaaaat atagtttttt ctaactcaaa agtattttcc atggtagaaa ttactttaat
    12841 tcatctgcca tttacttttt atcttattta ataattacta ttacttgaat atttacttct
    12901 ttaagtaaat ttcatagtta acaaagctaa aactattatt atagatgtta aaatacaaaa
    12961 ttgtaagtat tgttataaat atgcttacag ctacaaatat acatgatata ttaaaaagaa
    13021 gctgtaaatt attatcatac atacaagcaa ctatgcctct attaatccat gttaattttt
    13081 attatttaat acaaggagtt ccatgagaca tacatatcca ttcatatttt aaattatcca
    13141 attctcttat agatttcacc atctttttat aatcccaatt ccatggatta ggataaggaa
    13201 ctatatttcc attcttattt ttgactaaat cacctgcaaa taatacagct tcaaaaatca
    13261 tgcagacatg ccctggagta tgaccaggag tatggataat ttttatattc ccaatctgca
    13321 tattctctcc ataaggttca acatccttaa tgagccttct ggaaattgcc tttccaataa
    13381 acttcttaaa cccaggtcta tccttctctc ctataataaa aggtatatcc tctacatgag
    13441 cccatacttt tgctcctgtt ttttctttca ataatttaat attagatata tggtcaacat
    13501 catgatgagt taataaaata tgcttaatat ctgtaagttt aatgccaagt gtttttaatt
    13561 ctttaattat atttttcccc ataaaaggaa gacctgtgtc tataagaata atttctttat
    13621 caaacacaat aaaacaattt gagccacgag tactatctaa cattaataca ttttctttta
    13681 tcttcataaa taaattcccc ttatttggta tttt
//
LOCUS       DACULQ010000106        13607 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.106, whole genome shotgun sequence.
ACCESSION   DACULQ010000106 DACULQ010000000
VERSION     DACULQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.106"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..131
                     /locus_tag="JA154_12330"
     CDS             <1..131
                     /locus_tag="JA154_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996635.1"
                     /translation="ESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERRNE"
     gene            complement(306..626)
                     /locus_tag="JA154_12335"
     CDS             complement(306..626)
                     /locus_tag="JA154_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAT7996636.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(746..1483)
                     /locus_tag="JA154_12340"
     CDS             complement(746..1483)
                     /locus_tag="JA154_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAT7996637.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1698..3521
                     /locus_tag="JA154_12345"
     CDS             1698..3521
                     /locus_tag="JA154_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAT7996638.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3736..8034
                     /gene="polC"
                     /locus_tag="JA154_12350"
     CDS             3736..8034
                     /gene="polC"
                     /locus_tag="JA154_12350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087800.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time;
                     required for leading strand synthesis; PolC exhibits 3' to
                     5' exonuclease activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAT7996639.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8238..8699
                     /locus_tag="JA154_12355"
     CDS             8238..8699
                     /locus_tag="JA154_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAT7996640.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8729..9883
                     /gene="nusA"
                     /locus_tag="JA154_12360"
     CDS             8729..9883
                     /gene="nusA"
                     /locus_tag="JA154_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087802.1"
                     /note="modifies transcription through interactions with
                     RNA polymerase affecting elongation, readthrough,
                     termination, and antitermination; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAT7996641.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            9890..10171
                     /locus_tag="JA154_12365"
     CDS             9890..10171
                     /locus_tag="JA154_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HAT7996642.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10161..10472
                     /locus_tag="JA154_12370"
     CDS             10161..10472
                     /locus_tag="JA154_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7ae"
                     /protein_id="HAT7996643.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10494..12434
                     /locus_tag="JA154_12375"
     CDS             10494..12434
                     /locus_tag="JA154_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAT7996644.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12477..12857
                     /locus_tag="JA154_12380"
     CDS             12477..12857
                     /locus_tag="JA154_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HAT7996645.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12838..13515
                     /locus_tag="JA154_12385"
                     /pseudo
     CDS             12838..13515
                     /locus_tag="JA154_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
BASE COUNT         5427 a         1557 c         2563 g         4060 t
ORIGIN      
        1 tagagtcagc atcaaaattg gctgagatgg ttcatttatt ggctgttgaa aaggggataa
       61 ctgatataga agcatgggat gaagctgtga aagaatacag caaaatttat gaagaaagaa
      121 gaaatgaata gaatgataaa aaatgaaaac ttgatgataa aaaataactt tatttaaaga
      181 aattcaattt ttatagaagt attaaaaata atataaataa aaagatggta tttcaaatgt
      241 aagtaaaaaa tcattttggg ataccatcct tattatttat tttaataata tataagtaat
      301 aaatcttact tatccatatt ttttatgtaa cctctttttt ctaacttttt tatatgctcc
      361 ttacgtattt catcaagatt aataccatat tgttcttcta gcttaaacat catagtaaca
      421 catgtctgcg ctacatccat tagttcctta gcagttttct ttataatatc aacttcatct
      481 aaatcattat tttctccact tagatttctg aatttaccta tacattcagc taattcacct
      541 tgttcttcac ccattttgat aaatgtagat tctagtgttg ggtctaaatt attaagctct
      601 ggtaaagata tagtttttag tttcataaaa gctcctttca agttattcgt atttatacta
      661 aaacatcata acaaaaataa tactaaaaaa aaactttaaa ttcaacataa aaacttacat
      721 atataataaa aatcatgaaa taaatctata tttgtcttaa tacagcctca tataaaactt
      781 ctccaatagg tttatttatt acaagagaag ctgacttatc atagactgta tttgctttat
      841 taataagagc aatataatca cctttgtaat aatctataaa acttgctgct gggtacacag
      901 ctaatgatgt acctcctata atcaataaat cagcattact tatagcagaa attgttttag
      961 taatgacatc actgtcaaga gcttcctcat aaagtactac atctggtttt actatagaac
     1021 cacagttgtc acagtaagga atattacctc ccagatttaa catagattct aagtcaaaaa
     1081 acttaccaca tttagtacaa taatttctat gtacagaacc atgaagttct agcacatttt
     1141 tacttccagc catttgatga agtccatcaa tattttgagt tatgacagct ttaagcttac
     1201 ccatttcttc gagttttgct aaagctatat gagcattatt gggttttgca ttaggatata
     1261 ttaatttatc cttataaaat tcaaaaaatt cttctggata tctaacaaaa aaagtatgtg
     1321 aaactaattg ttcagcagta aattgtttat tgagtttttg gctaaataat ccagtagaac
     1381 ttctaaagtc tggaatatta gattcagttg aaacacctgc tccaccaaaa aatacaatat
     1441 tattgtgatt agctattaag tcttttaagc tatttgcatc catagttaca cctccaaatt
     1501 attatatata aattgtacta catattaggt cgtttatgtt ttattaattt aaataaatat
     1561 atagtttaat aagttaaata aagggaatat aaataaaatg tatacagagt ataaatagac
     1621 aatatttact aaaataacaa gttgtcgtat aattatatta aaattgtttt agtgtataag
     1681 aagggcgggg gaagtacgtg aagaaaaaag cagctttagc aacattggct atgttaccct
     1741 taggtgtggt aaatgcacat gctgatggag atataggtat agtgactata aattatttaa
     1801 atgtaagaaa tgaaccaact gccgaaagta gcatagcctt tgttgctaaa aaagatgata
     1861 aagttttgat taaagactct tctaatggat ggtataaaat aaaagctgaa tctggacaag
     1921 aaggttgggc ttcatcaaaa tatatagcaa aatcaaatag tgactcttta agaacgtcta
     1981 caaataaaga aaaacaagtt atttcaaata gtttaaatat gagaaatgga gcaggtacaa
     2041 gttacagagt aataactgta ctaaaaaaag gtcaaaaagt agaagtaata tcagagagta
     2101 atggatggtc taaaatcaaa tatgatggaa gactaggata tgtatctagc tcttatttag
     2161 gagacgtttc aaactcaact aataaatcaa aaactaaaca agtgaataca acttcactaa
     2221 atgtaaggag tggaccaaat acaagttatg gtttgttagg caagttgcca aaaggaagta
     2281 aagtagaagt aatatcagaa agtaatggat ggtccaaaat aaagtataat ggaaaagatg
     2341 cctatgtatc tagtatgtac ttatcggatg taagtcaaag taattcagac aattctagtc
     2401 aaagcaatga taaaaagaat actgataagg ttgtaaatac agcttcttta aatgtaagaa
     2461 gtggaccagg ttctacatat agtaagttgg gaaaagttta taaaggaagt aaagtaactg
     2521 tactatcaga aagtagtgga tgggctaaga ttaattttaa caataaagaa gcatttgtag
     2581 taggcaatta tttatctacc tcagcagata cttcaaataa taactcaaat agtaactctg
     2641 ataacagttc taatagtaat ggtaataatt cttcatcatc aggccaagta aatggtatgt
     2701 caggtataag tggggctaaa attgattata aatctttgag ttatacttta gaatctcata
     2761 taagtaaaca agttgagaaa gcagcatcag gaggaaatgt gatagctcca agtaatagga
     2821 aaagtactcc aagtcctgag tttagtactt tttcagctca aagaacaagc tcatttgtaa
     2881 atgcaagttc aagtgatata gaatattatt taaatcctaa gaattttaca aatactacta
     2941 aaggtatgat gcagttttta aagattaaca gttatagaga tggtatttca gaatcaagtc
     3001 ttaactcgta tcttaatggt ttatcttcta gtgtatttaa aaaccaggga gcagccttta
     3061 taaatgctgc caagaagtat aatatagatg ttgtatatct agtatctcat gctatgtggg
     3121 aaactgctta tggtaagtct acacttgccc aaggtcaaac attaacatct tataagggac
     3181 aacctcttag taagccagtt aaagtataca atttttttgg tataggagca atagataaaa
     3241 gtgctaatgt ttcaggtgca gaagcagcgt attccaatgg ctggactagt gtagaagcta
     3301 caatagatgg ctctgcaaag tggatatcac aaaattacgt aaatagttct aagtacaatc
     3361 agaatactat ctacaagatg aagtggaatt atgattatac atggcatcag tatgcaactg
     3421 atgtaaactg ggctaatgga atttctggaa ttatggaaaa tttaattggt ctttatggag
     3481 gaggaagtag tttggttttt gaagttcctc aatataagta atacataaat aaaaaatata
     3541 gactatgggt agttttcatt ttatttaaat tatctatagt ctttttgtat ataatttata
     3601 atctgtaaat ttaatgttaa caaaactttt atgttataat attaattcta agtaaatata
     3661 agtgagtaat aggtagaaat agataaaaag tggtaataat atatataaag ataattttga
     3721 gaaaggagga tatatatgga aagtattaag gaatatttgg ataagttaga aattaataat
     3781 agtggtcttg gaaagcaact taaagaagtt tatataaata gagtagttta ttttaaggaa
     3841 gataagatag tttattttta tctaacttct aaagatattg tttctcatga acttttagat
     3901 aaatttaaag aagaattgat gtataaactc gattatttta aagatatgag gatgaagata
     3961 agatttactg gtcttgaaag aaaatccaat aaagatgtta taaaaaaata ttggaataat
     4021 atattgtata tattaaagta tctttgtcca tctattgcag ggtggtataa acaagtagaa
     4081 tttctttgtt tagaagaaga attaaaaata aaacttccaa aaggtatatt ttatgagcga
     4141 ttaatgaaaa aaaatgtaac ttatgtactt aaaaccgtgc ttagcgaaga gttgggatta
     4201 gatttaaata taacaataga aaaagctgtt gatgaaaagg taaataaaga aagactcata
     4261 agaatcaatg atagagaaat ggaagaaaaa attagggctt tagaaattgg aaaagttaat
     4321 aactgtgaag aaaatgaaga ggaaagctat gtcattaagt ctgatgtaga tgaaaattta
     4381 atctatggag ataatgcaaa tgctatgatt gaaaatatag ttgaattaaa tgcatcttct
     4441 ggaacagttg cagttgttgg agatatattt gatgttgaca ctaaagaact taaaaacgga
     4501 aaaatactta tgatagcttc aattacagat tatactagct caataagctg taaacttttt
     4561 ttaacagata caaataagga tggagtactt gaaaatgtta aaaaaggagc atacttaaaa
     4621 atcaagggtg acatagttta tgatacatat caaagagaaa ttagtatgat gattagtggg
     4681 ataagaaaag aaactagaat agagagagaa gataaatcag aggaaaaaag agtagaactc
     4741 catgcacata cacaaatgtc ttctatggat gctatttgtt ctgttaaaaa gcttgtagag
     4801 cgtgcagcca aatggggtca ttctgctatt gctataacag accatggtgt tgtacaagga
     4861 tttccagatg ctatgaatgc aggtaagtca aataatataa aaatacttta tggagtagaa
     4921 gggtacctgg ttgaagatga ttctctcatt atagaggatg caaatgataa agaactttcg
     4981 cagacatttg ttgtatttga catagagaca actggctttt ctaatacaaa tgataaaata
     5041 actgagattg gtgctgtaaa aatagaaaac tttaagattg tagatagatt tagtgagtta
     5101 ataaatcctg aaaaagatat ttcttataag atacaggaat tgacaggaat aactaatgaa
     5161 ttaattaaag acaagccaac cattgaagaa gttcttccta agtttatgga atttgttgga
     5221 gatagtgtat tagtagcaca taatgcagag tttgatactg gatttatatc gcaaaaatgt
     5281 agggaacaag gcttgttata taataacaaa aaagtagata cacttatgtt agcaagagta
     5341 atgttgccaa acttaaaaag acatagatta aatgtcgttg caaaagaact tggtattcct
     5401 cttttaaatc accatagagc agtggatgat gctgaagcta cagcacttat atttaataag
     5461 tttttgcaga tgcttacaga aaaaggtgca aaaactctaa gtgatgtaaa caatatactt
     5521 ggaaagattg attacacaaa gcttagcaca aatcatataa ccttgatagc aaaaaattct
     5581 ataggtataa aaaatttata taaaataatt tcagatgcac atgtaaatca tttttttaga
     5641 gcaccaagaa tccttagaag tgtattaaat gaatataaag aaggattaat aattggttcg
     5701 gcttgtgaag ctggggtggt ttttcaagct gtaaagaaaa acgtgtctga tgaggaaatg
     5761 aaaaaaataa tagatttata cgattatatt gaagtaatgc ctatagataa taatagattt
     5821 atgattgata aaggagaagt aaaggacgaa gaagagttaa gggagttgaa taggaaacta
     5881 attgatacag ccaaaaaatt tgacaaaatt ccagtagcta ctggggatgt tcatttttta
     5941 gacaaacatg aagctgtctt aagaaaagtt cttaaatatt ctcaaggttt taaagtagat
     6001 gaagaagaaa cttatttaca ttttagaaca acagatgaaa tgttggaaga gtttagttat
     6061 cttggagaag aattggcata tgaagttgta gttgaaaata gtaatctgat tgccgatatg
     6121 gttgaagata taaaacctat accagatgat acttatcctc caattataga gggctcagat
     6181 gtggagttaa gagaaatgtg ttacaaaaaa gccaaacgaa tctatggaga cccaatacca
     6241 gaaatagttc aaaaaagact ggatagagaa ttaaattcaa taataagcaa tgggtatgca
     6301 gttatgtata ttatagctca aaaactggtt gcaaaatctc tttcagatgg atatttagtt
     6361 ggttcaagag gctcagttgg ctcatctttt gcagctacta tgagtgatat aacagaagtt
     6421 aatcctcttc cagcacatta tatttgtgaa aacgaagact gtaaatattc attcttttat
     6481 gaaataggtg aatggggctc aggtgttgat ttaccagaca aggattgtcc aaagtgtgga
     6541 agaaaacttg ctaaaaacgg tcatgatatt ccatttgagg ttttcttagg ttttgaaggt
     6601 gataaggaac cagatataga cttaaatttc tcaggaaatt atcagccaac aattcataaa
     6661 tacacagaag aactatttgg agaggggtat gtatataggg ctgggactat aggtactgta
     6721 gcagaaaaaa cagcttttgg atacgcaaga aaatatgtgg aagaaaataa tataagtgta
     6781 ccaaatgcag aagtacttag gctttcaaat ggatgtactg gagtaaaaag aacctcagga
     6841 caacatcctg gtggagttat ggttatacca gactataaag atgtatacga ttttacacct
     6901 atacagtatc cagcaaatga tacttcttgt ggtgttataa ctactcactt tgattatcat
     6961 tcaataagtg gtagaatact taaacttgat atacttggac acgacggtcc taccattata
     7021 aggatgcttg aagatatgac tggaataaat ataacagaaa ttcctttaga tgataaagag
     7081 actatgtctc tatttacatc tacagaagca ttaggtgtta caccagaaga aataaactgt
     7141 cctataggtt gccttgctgt accagagttt ggaactaagt ttgtacgaca aatgttactt
     7201 gacacaaaac ctactacatt tgcagaactt gtacgtatat ctggtctctc tcatgggaca
     7261 gatgtttggg taaataatgc acaagactta gttagagaag atatagttgg attaaaagat
     7321 gttatatcta cacgagatga cataatgaac tatttaatat ttaaaggatt acctcccaaa
     7381 atgagcttta caataatgga gagtgtaaga aaaggtaaag gtttaaaacc agaacacatt
     7441 gaagaaatga aaaaaaataa tgttccagaa tggtatatag gctcatgtaa aaagattaag
     7501 tacatgttcc caaaagcaca tgctgttgct tatgtaatga catcatttag aattgcatat
     7561 tgtaaagttc actatccaga agcattttat gcgacttatt ttacgacaaa agttgaggat
     7621 tttgatgcag atttaattgt taaaggatta gatgctataa aatcaaaaat aaatgaaatt
     7681 gagtcactgg gaaatgatgc aaccacaaaa gaaaaaggta tgttgaccgt tttagaagtt
     7741 gctcttgaaa tgtatgctag aggaataaaa ttattaccag ttgatatata taaatcagat
     7801 gcaactgagt ttatagttgt aggagaaaaa acattacttc caccaatggc agcaatacaa
     7861 ggtcttggag gaaatgcggc tataaacatc caaaatgaaa gaaaaaatgg tgaatttata
     7921 tcaaaagaag atttgagaaa gagaacaaaa atatcaaaaa cggtaataga aactcttact
     7981 aatcatggtt cacttgagaa tatgagtgag agaaatcaat tgtcattgtt ttaagttagc
     8041 ccaaaaagtt gcaaataaaa gaattttatg atatcatagt atagaaaata gtatactttg
     8101 cagaaactaa gagtgagaca gctgttctca ctcttttatt ttaaatatat tattaaaaag
     8161 ctggtttatt tttattaaat atattttatt taagattata taaactggtc atttacaatt
     8221 gaataggagg aactttaatg aaaaaaaatt tagaagctac aattgaggag attgtaacta
     8281 aaataacgga tgaacatgga tttgaaatgg ttgatgttga atatgtaaaa gaagcaggag
     8341 aatactactt aagagtatac atagataaag aagaaggaat atctttaaat gaatgtgaat
     8401 tagttagtag agaattaagt ccaatactag atgaaaaaga ccctataaaa gaaaattact
     8461 ttttagaggt atcttctcca gggcttgata gagcattaaa aaaagacaga gattttgtta
     8521 gatatcaagg tagagatgta gatttaaaat tatataaacc attaaatgga tgtaagcaat
     8581 ttgaaggtga acttgtagga ttaacagaag ataataatat aaaaatcatt gcaaatggta
     8641 aggaaatgga atttaataga aaagatgttg ctattgtcag attagcaata aagttttagt
     8701 ggtatattaa atagggagga tataaaaaat gaatcatgaa tttatggaag cattagatga
     8761 attagtagtt gacagaggta tagataaaga aatacttata gatactatag aacaagctct
     8821 tttaactgca tacaagaaaa actttggttc agcacaaaat gttagagttg aatttgatag
     8881 agacaaggga gatataaaag tattttctca aagagttgtt gttgatgaat cagatttata
     8941 tgatactttc ttagaaatag agttagcaga tgctagagag ataaatccta attatgaatt
     9001 aggtgatatt atagaacatg aagttacacc taaaaacttt ggaagaattg cagctcaaac
     9061 agcaaagcaa gtagttgttc aaagaataag ggaagctgag agagaaatag tgtataatga
     9121 atttatagaa aaagaaaatg aaatagttac aggagaggtt gctagagcaa ataaaaatgt
     9181 tgtttatgta aatcttggaa gaatagaagg tataatgact caaagtgaac aaataccatt
     9241 tgagaattat aaagcaggtc aaaaaataaa attctatata cttgaagtta aaaagactaa
     9301 caaaggacct caaatagttg tatcaagaag tcatccgggt ctagtaaaga gattgtttga
     9361 agaagaagtt cctgaaatat atgaaggaat agttcaaata aaatctgtag caagagaagc
     9421 agggtcaaga actaaaatgg cagtaagatc tatagatgaa aaaatagacc caattggagc
     9481 ttgtgttgga ccaaaaggac ttagagtaaa aaatatagtg gatgaacttg gcgatgagaa
     9541 aatagatata ataaaatata gtgaagaccc agcagagttt atatcagcag cacttagccc
     9601 atcaaaggtt gttagagtag atatagatga agatgaaaag tctgccttag taatcgttcc
     9661 agattatcag ttatcgcttg ctataggtaa agaaggtcaa aatgcaagac tagcagcaaa
     9721 attgactaac tggaaaatag atattaagag cgaatctcaa gcagaacaag aaatgttaaa
     9781 tcttgaaaat tccaaagaaa tagaagctga ggatgttgaa gctgaattta tcgaccaaga
     9841 agagttagat acttcagagg ataattttag tgaagaagag taaggaggga tgattttgaa
     9901 aaaagtaaaa aaagtgcctc aaagaaaatg catagcatgt caagatagag actctaaaaa
     9961 agagcttata agaatagtta aaaataaaga aggaaaaata tttttagacc caacaggaag
    10021 agccaatgga agaggagcat atgtatgtaa aagctctgaa tgtctaaaaa aagctataaa
    10081 gagtaaagca ttaaataaag cttttaaaat agatgttcct gatgaagtct atgataatct
    10141 acttatggag ttagaagaat atgaaaaata ataaagaaaa agtactttcg ttcttagggc
    10201 tcgcaactcg tgcagggaaa atagtgtctg gtgatgattc aactttgctt gaccttaaaa
    10261 aaggaaaagt aaatttgatt ttaatcgcag aagatgcatc aaataatact aaaaaacttt
    10321 ttaaagataa atcaactttt agaaatattc catatctatt tttttcaaca aaagaggaaa
    10381 taggatttgc aataggaaaa tctccaagag cagttgtagg aataaaagat gaaaattttt
    10441 ctaaaaagat aattgaacta atagaaattt aaaataaaat agggggtgat cttgtgtcaa
    10501 aaacaagagt ataccaaata gcagaagaat taaatatatc aaatgaagag cttataaata
    10561 agttagctga attagatata aatgtaactg acaaagattc agtattagaa ggtgaagagt
    10621 tagaattagc acttgaaatg ttaggtgaag atttatctca agaaaatgga aatgttatag
    10681 aaatagatgg aaagttgaca gtacaagtat tagcaacgaa attagataaa tctccatcag
    10741 aaataattat gaagcttatg aaaatgggaa ctatggcaac aataaatcaa gagataagtt
    10801 ttgagattgc tgctttggct gccaaagact atggatttga attaacagta gctgaaagtg
    10861 atgatacaga agctttagaa atagaagctt taatggaaat tgaagaagac aaagaagagg
    10921 atttaaaacc tagaccacca gttgttacag ttatgggaca tgttgaccat ggtaagacat
    10981 ctttactaga tgcaataaga aaaactgatg tgatctcagg tgaagctgga ggaataactc
    11041 agcatatagg tgcttcagaa gttaaaatca atggacacaa aatagtattt ttagatactc
    11101 caggacatga ggctttcaca tcaatgagag caagaggagc acaagttaca gatatagcaa
    11161 tattagtagt tgcagcagat gatggtataa tgcctcaaac agttgaagct ataaaccatg
    11221 ctaaagcagc aggtgtacct ttaatagttg ctattaataa aatagataaa ccaggtgcaa
    11281 acccagataa ggttaaacag gaattagcag accaaggatt attagttgaa gactggggtg
    11341 gagaagtaat agcagttcca gtatcagcta agaaaaaaga aggaatagat acacttttag
    11401 aaatggtatt attggttgct gaaatggaag agttaagagc taatccaaat aaaagagcag
    11461 taggaactgt tatagaagct gagcttgata aaggaagagg gccagttgca acagtacttg
    11521 ttcaaggtgg tactttaaca gtaggtgacc caatcgttgc aggtgttgca tgtggtaaag
    11581 ttagagcaat gataaatgca aaaggtaaga gagtaaaaac agcaggacca tctacagcag
    11641 tcgagatatt aggtctatca gaagttccac aaggaggaga ccaatttgtc gaagttccaa
    11701 ctgataagat tgctagaagt gtagcagcaa gaagacaaca aatagttaga gatgaaatgt
    11761 taaaatcaac tcaaagatta tcactagatg ctctatttag tcaaatgagt gaaggttcta
    11821 taaaagacct taatatagtt ataaaagcag atgtacaagg ttcagtacag gcagttaaac
    11881 aatctttaga aaaattatca aatgaagaag ttcaagttaa agtaatacat ggtggtgttg
    11941 gagctgtaac agaatcagac atattacttg cagcagcttc taacgcaata ataataggat
    12001 tcaatgtaag accagttcca ggtgcagaat ctctaggaga aaaagaaaat gttgatataa
    12061 gaacatatac tataatatac aaggcaatag aagatataca agcggctatg actggtatgt
    12121 tagaccctga atatgttgat gaagaaacag gaaaagctga aataagagaa atatataaaa
    12181 tatctggtgt tggaacagta gctggatgtt atgtgactaa tggtaagata tttagaaatt
    12241 gtaaagtaag attagttaga gatagcataa taatacatga aggtgagctt gcagcactta
    12301 agagatttaa agatgatgta aaagaagtta attctggata cgaatgtggt atgagttttg
    12361 taaattataa cgacataaaa gaaggcgata tagtagaagc ttatataact aaagaagtag
    12421 aaagaaaatt ataaattcgt atcaaagaaa atcagttagt aaagaaggtc gatggtatgg
    12481 catcatataa tagaacaaga aggatagcag aagaaataag aaaagtcgta agtactatgc
    12541 ttataaatgg agtaaaagac cctagaataa catctatggt gagtgtaact gatgtagaag
    12601 ttacaaatga cctaagatat gcttatgtat atgtaagtat tctaggcgga gatgaagaat
    12661 ccacactaac aggtcttaaa agtgctggag gatatataag aagagaagta ggaaaaaata
    12721 taaaacttag atatattcct gagatagtat ttaaacttga tgattcaata gaaaaaggaa
    12781 tgtatatgga tagccttata aaaagagtta atgaaaaaaa tgctcaacaa aatgaggatg
    12841 aaaattatga tgaataatat ggatagtatt ataaactata tttatgaggg tgactatttt
    12901 atagttacct ctcatatcag tccagatgga gacaatattg gttctacact ttcaatgtat
    12961 tatacattga agaatttagg aaaaaatgta tattatgtac ttgatgatga agctccatta
    13021 aatttaaggt ttttggttga aggtgtaaaa atctataagt ctaatgaatt taatatgaag
    13081 aactattctc taattgcact tgattgtggg gataagatga gaatatgtgt gagtgatgaa
    13141 atcaaaaata atgcatcaaa attaatctgt attgaccatc atgctagtaa tgattcatat
    13201 ggagatttaa attatgtaga tattgatgca tcttctacat gtgaattagt ttataattta
    13261 ttagttagat ttcagcaaat aaaagatatt aatattataa atgaagatat agcaacatgt
    13321 ttatatacag gtcttgtaac tgatactgga aacttttcgt attcaaatgt acatgcaagt
    13381 agctttgaaa tggcaaaaaa tttacttgtt ttaggtgcac aaaaaaatac tataatacaa
    13441 aatatatatc aaagcaattc ttctggttat tacaaattat taggagaagc tctaaagggt
    13501 ttggagatat ttgattaaaa aatagttacg cacactcgtg actttagtca tgagttagta
    13561 gctaaaatag tcagcatata gggaaactta tatgtagaga taggata
//
LOCUS       DACULQ010000107        13582 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.107, whole genome shotgun sequence.
ACCESSION   DACULQ010000107 DACULQ010000000
VERSION     DACULQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.107"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            194..778
                     /locus_tag="JA154_12390"
     CDS             194..778
                     /locus_tag="JA154_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996646.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(882..1661)
                     /locus_tag="JA154_12395"
     CDS             complement(882..1661)
                     /locus_tag="JA154_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAT7996647.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(1719..2975)
                     /locus_tag="JA154_12400"
     CDS             complement(1719..2975)
                     /locus_tag="JA154_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAT7996648.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            3233..4132
                     /locus_tag="JA154_12405"
     CDS             3233..4132
                     /locus_tag="JA154_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAT7996649.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            4156..5604
                     /locus_tag="JA154_12410"
     CDS             4156..5604
                     /locus_tag="JA154_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAT7996650.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDIEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            5663..6469
                     /locus_tag="JA154_12415"
     CDS             5663..6469
                     /locus_tag="JA154_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996651.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            6495..8414
                     /locus_tag="JA154_12420"
     CDS             6495..8414
                     /locus_tag="JA154_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996652.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(8656..9003)
                     /locus_tag="JA154_12425"
     CDS             complement(8656..9003)
                     /locus_tag="JA154_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HAT7996653.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(8990..10918)
                     /locus_tag="JA154_12430"
     CDS             complement(8990..10918)
                     /locus_tag="JA154_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HAT7996654.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(11329..13518)
                     /locus_tag="JA154_12435"
     CDS             complement(11329..13518)
                     /locus_tag="JA154_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide synthetase"
                     /protein_id="HAT7996655.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         4929 a         2086 c         1747 g         4820 t
ORIGIN      
        1 atttgataaa taaaaactta acactattta catattgtaa atttggccat ttactgttat
       61 tatgaatatg gttgaaatta tttaaataaa tactgttgac ttagtttcat atatttacca
      121 ttaaactttt atcataaata tcatatcata ataatatatt taataagtct aataaactat
      181 acgaggtgta aatatgttaa tgaataccca ttttattatg gcaaaatcaa tacttgataa
      241 tatagatgaa aataaaacat tctttataag cgaaaaaaat tttatatatg gcaatataaa
      301 gcctgatgct ttttctaaat ataaattaaa aaaacattat ttagatgagt catttaatat
      361 gattgtttct aaaataaact atctatgcag tcttaatata gatagtcttt ctaaatactt
      421 ttcagtaagt agacttagtc aggaactagg agtcatatgt cattttttat gtgatttttt
      481 ctgtgtagct cacagtgaac gttgggagtt taagcatagc ttaaataaac atgttactta
      541 tgaaagagaa cttggagcat ttgcaaaaga tgttgattta tctaaaatta aatgtggtac
      601 tataaatagt agttttgaga agttttttac taaactttat agcgattaca aaaagaaaac
      661 tgattatatg aatgatttaa aattttcagc ttatacttgt aatagtgtca taaattatat
      721 acttgactct attttatcta atacaataaa atcatatcat atagcaaatt gcggttaact
      781 taagaatttt taaacttata taaacttata attgaatata attaaaatga ctgctctata
      841 gaatataatt ttatacttct ctaggacagt cattttttat actaactatt tatctccatt
      901 ataacatcta tggcactttt tacactatct actacataat ttgcactttg ctttacatct
      961 tcccttgcct tagacattgc aaaactatat ttaaatcctt ctaacatgca aatatcattt
     1021 ggagaatccc caatagtagc aacttcatct ggacttacat ttaacgtttt tattacatgc
     1081 tctatacttc ctctttttga cacactttta ggaactatat ttaaagtata tgttccagaa
     1141 aaatatattt ctaagacttc actaaactca ctttttagat aaagttctat tctggataac
     1201 tcttccaaat tttcagaaca tacgcctata tttcctataa gattatcaaa actacttctt
     1261 ttttcaaagt ctgggtcaac tataaagtca ctagaataat gtctaaaagc agtggtatca
     1321 ctacccacaa ttacatcacc cttatattta aaataaagtt gtgcatcact tgactcaaat
     1381 ctatcaatga ctttttttac taaattatca tctatcgttc ttttaaaaat tacattataa
     1441 tctttatcat atataaatga accattttca catacatagt atccattcat atctaaacta
     1501 tctttgataa tattatgtgc ttgtttaaaa attctacctg tagcaacatt aaactgtact
     1561 cccttttttt ctatttcttc aattgctact atatcttctt ttgttatggt tccattttca
     1621 tatagagttc catctaaatc acaaaatata tgttttatca taaattaaaa gcctccaaaa
     1681 acaattttat attttactaa tactttaatc aaaataactt aaagcttttc taattgtatt
     1741 ctcaactcat taagcaaatc tctagtagct cttaatctta tctttcttct atccccatta
     1801 aatatatttt tctttactat tgtttttcca tttatatata ttgcagtata aaccagtccc
     1861 acaggcttct ctttggttcc tccaccaggt ccagctactc cagttgtaga tatacctata
     1921 ttagctttaa gtcctttagc aacgccctca gccatttcta tagctgtttg ctcacttact
     1981 gctccaaact cttccaaagt ttctcttttt actcctaatc ttttcatttt agaatcatta
     2041 gaatatgtta cacaaccttc aagaaataca gaggatatac ctggatagtt tataagagat
     2101 gaagaaacca aacctccagt acaagattca gccactgcta ttgttagatt tttttctaca
     2161 agtagcttag aaacagcatc ttctaaagca gtctcccctt cagatatgga aatatctcct
     2221 tcagtatata catatttacc aactctactt tttatttttt cttctactgg ttttattaat
     2281 ttaaaagctt ctctttcatt ttcagccttt gcagttattc ttattgtaac ttctaattct
     2341 tttgcatata aagcaactgt tggattagtc tgctcattaa ttatatcgag aatttctaat
     2401 tctagatttg actcacctat tccatataat cttaaagtct tagatactag catttcattt
     2461 gtgaattgtt gtaagtaagg tttgacactc tcattaaaca tagctttcat ttctcttgga
     2521 ggtccaggca atactattat aaatttacca tcttttttta aaattgcccc tggagctgta
     2581 ccattattat tttttagtac tatagcatct tttggaaagt acacttgttt catattattt
     2641 tcggccattt ttttccctgt tttaacaaag aaagattcca aaacctctag tgaaggttta
     2701 tgtagttcta atttctgacc aaagtattct gcagcagttt cttttgtcat atcatcacca
     2761 gttggcccaa gaccacctgt agtaattaca aaatcacttc tctttaagct ttcatcaaaa
     2821 cattctaaaa gtctttgttt gttatctcca acagtactct gatggtatac ttctattccc
     2881 aatgatgcaa gttctttagc taaaaattga gaatttgtat ttactatatc tcccaataat
     2941 atttctgttc ctacacttat tatttcagct ttcaaaattt atcactcctt gatttatagc
     3001 cattaagact agtcttaagt tcataacaat aattttagca aaaataataa cataaataaa
     3061 gttaaaaaat catttaaaca tatctttaaa ctagattatt attctaaaac cacacataat
     3121 ataaacttcg tatatagtgg tactttcatc ctcaactgtt aattcatata attaagtatg
     3181 taaataaaaa gtttacttta aaataataaa aattaaaata ggagtgtata atatgaaaat
     3241 agcagttata atgggtggaa tatcatcaga gagagaagtt tctttaaatt caggaaaaga
     3301 gatatataat aaccttgata aaaataaata tgaagttgta aaagtaataa tagatgataa
     3361 aaaagatata tttacaaaga taccagaaga tatagatttt gcaatacttg ctttacatgg
     3421 aaaatttggt gaagatggat gtatacaatc aatacttgag actatggata taccatactc
     3481 tggatgtgga cctctttgca gtgggatgtg tatggataaa aatataacta aaaaaatgct
     3541 tagagattca aacctaccta ctgctccatg ggttttagta aaatcagttg atgaaataga
     3601 ctatgatgaa atagataata taggttatcc agtatttata aaaccaaact caggaggttc
     3661 aagtgtagcc acattcttta ttcattctaa ggatgaagtt gaagaagcag ttagaaaagg
     3721 tctggaagtt gatgaatttg taatgattga aaaatatata ccaggtggag aatatacttc
     3781 attcatatta aatggagaag tgttccctac aatatctata aaatctgatt caggattctt
     3841 tgattatgaa gctaagtact cagttgaaaa aggtgcaaag gaagaagttg tttacttaga
     3901 tgaagaactt caaaaaagag taaacgaaat ttctgagact tgttggaaaa tttttaattg
     3961 caaagcatat gtaagagttg atatgattat aagtgaaggt attccatatg ttcttgaact
     4021 aaatactctt cctggaatga ctcaaactag tttaattcct agaagtgctg ctgctagagg
     4081 tattaagtac agtgaactat tagataaact tatagaatac tctttaaatt agatgaaata
     4141 atttgaggtg gtaatttgat attttcaaat ctaaaactaa atgataatga accaatttat
     4201 atacaactta aaaattatat aagtgatatg atatcaaaag gactaattcc agacaatagt
     4261 aagctccctt ctacaaggga gcttagtcag cttttacaag ttagccgaaa ttctgtagtt
     4321 ttaacttatg aagaattaaa atcagaagga ttaatctact ctatttcagg aaaaggtaca
     4381 tttgttaaat caaagaataa gtcttcaaat actacatggt cattaaactg ggattgttta
     4441 gagaatacat actctaaaaa agccaatgaa ttggacatta taaaaagtga aattccttgg
     4501 agctctgacc tgatttcctt taaaagtatt tctccagatg gagatttatt cgacatagaa
     4561 gaattaaaaa aatccttttt aaatagaatt tcactagaag gacataaatt actcaactat
     4621 ggatatgcac aaggttataa gccccttatt gactatttac tagaatatat gacaaataag
     4681 ggtgctgata taagtaataa agatatacta ataacaaatg gattcacaga aggctttgat
     4741 attattatgt catcttttac ccaagaaaaa gactacataa tatgtgaaaa tcctactcat
     4801 aatactgcta taaaaattat gaaatctcat aacctcaata tcttaggtgt agatataaat
     4861 gaagatggtt tggacttcaa tatgctagaa gacaatttag ttaagtataa aaataaaata
     4921 aagtttgcat acattacacc ctcttatcac aatcctacag gaattgtaat gactcctgaa
     4981 aataggtaca aattctataa tcttatgaaa aaatacaata tagcaattat agaagatgga
     5041 tttaatgaag aattactcta caatagttct catatatttc ctatctgttc cctagataat
     5101 atgaataatg gggtcgtata tattggtagt ttttcaaaaa ttttatttcc tggaatgaga
     5161 attggatgga tatttgcaga taaaaaagta atcaataagc tagaaagtgt aaaaaggtgc
     5221 agaaacatac atgtatcctt tttggaccaa ggaattttat ttgattattt aagtaatgga
     5281 ggttttgaaa aatacattaa aaaaatacgt aaattctatg gggacaaatt taactttgca
     5341 tataagtgta taaaaaaata tataaaaaca gaatatatct tgggtgatgg tggtttgcat
     5401 atatttataa aactaaaaaa tatagatact agagttttat tagaaaaatg ttatgaaaaa
     5461 aatgttatat ttatgcctgg agatttattt tttacagata actctggttt tgatacttta
     5521 agactcggat tttctaggtt gtcttttgaa gacattgaaa ttggaataaa aataattgga
     5581 gaaactataa atgaaatgtt ctaggttaac taaatattgt tagcctagtt tatttttatg
     5641 aggtataata tatatagttt atatgaatct aatactgggg gataatatga gtaaaaagtc
     5701 aaaacgaaga aaaattaata aattaaaatt aacgattgta ataactgtat tatttcttgc
     5761 tttattatct atatatgaga tactgttctc taatcccaac aaaaaaggta atataaagag
     5821 tcaagaaact atgcaaaata aaaataatac aacagatgat tctaaaaata atcaaagctc
     5881 taccaaaaag caggaagata ataatatcaa gcctactttt tcatattctt caataccaga
     5941 taatataaaa aataaaatga ttggtaaatc tatgcctact gatgaaccta taagctttga
     6001 tagtctatct tatttaaaac ttacatatta tggatttgat gaaaaaactc atcaaggaga
     6061 aatgatagta aacagtgaac tagctccaga agtagtggat atttttaagg aattatacga
     6121 aaaaaaatat ccaattgaaa aaattaagtt aatagatgac tatgatgctg tagatgaaaa
     6181 atctatgtct gataataata cctcatcatt ttgttataga actatagctg gtacaaatgt
     6241 agtttcaaat catggtaaag gacgtgctat agatataaac ccattacaga atcctcaagt
     6301 ttctggaaat gatgttacac ctaaagtaag tacagtttat gctgataggt ctagcacaaa
     6361 atttggaatg ataaaaaaag gtgatgattg ttataatgct tttgtaagca gaggttggag
     6421 ttggggtggc tattggaaaa acccagatta tcaacatttt gaaaaataat agattagaaa
     6481 taaaggtgaa ttatatgaat ttaatgacat tggaaaatat aagtaaaagc tattctgaaa
     6541 aaaaattact agaaaatata tctcttggaa taaatgaagg ggaaaaaata ggacttattg
     6601 gtgtaaatgg tactggtaaa tctaccttac tcaaaattat tgcaggagtt gaagaaagtg
     6661 agactggtaa tataacaaaa actaatggta ttaggataga atatctacct caaaatccaa
     6721 cttacaatga aaattcaact gtacttgaac aagtatttaa tggaacttct gaagaattaa
     6781 agctacttgg tgaatatcaa gaaatcctag aaaaacttaa ttcaaacttt acagaggaat
     6841 taaataaaaa attattatca gtacacgaaa aaatagatgc cttaaatctt tgggatttag
     6901 aaagtgaagc taagtcagta cttacaaagc ttggtataaa tgactttaat caaaaaatag
     6961 cagaactttc tggtgggcaa agaaagagag tctctctggc atcagccctt attacacctt
     7021 gtgagttttt gatactagat gagcctacaa accatctaga caatgatact atagattggc
     7081 ttgaggaata tcttaattcc agaaaaggct cattgcttat gataactcat gatagatact
     7141 ttttagaccg tgttacaaat agaattattg aactggacaa aggtagatta ttcagttatg
     7201 atgggaatta ttcaatattt ttggaaaaga aaatggaaag actttcgcta gaagcaagta
     7261 ttgaagataa aagacaaaat ttaataagaa cagagcttgc gtgggtaaga cgaggagctc
     7321 aggctcgtag cactaaacaa aaagctcgtt tacaaagatt tgatgaactt ataaatagag
     7381 attcttttaa acctgatgaa aatcttgata tatctgttgc atcaagtaga cttggaaata
     7441 aaataataga aatacacaat atatctaaat catttgaaga aaatactgtt atagataatt
     7501 tagaatacac tttaactcgt acagatagaa ttggtataat aggcaaaaat ggtatgggta
     7561 aatctaccct tataaatata cttaatggag aaatagagcc tgatagtgga tatatttcaa
     7621 ttggagaaac ggttaaaata ggttgttttt ctcaagacga ctctcatatg aatattgata
     7681 tgagggctat agactatgta aaagaagcta gtgactatat agaaacttca gatggtacta
     7741 ggattacagc ttctcaaatg tgtgaaagat tccttttcaa tggtactatg caatatactt
     7801 tgataggtaa attatctggt ggtgaaagaa gaagactaca tcttcttaaa atccttatga
     7861 cagctcctaa tgtgcttctt ttagatgaac ctacaaatga tttagatatt gaaacgctta
     7921 aaatattaga agaataccta gacgaattta aaggtgtagt agttacagta tcacatgata
     7981 gatatttctt agatagaatt tgtaataaaa tatttgcata tgaaggtaat ggaaaaattc
     8041 atatctatac aggtaattac agtgattatc ttatttatag agagattcaa ggaattgaat
     8101 ttgaagaatc taaaaaggaa tcagttaaaa aagatgactc tactacccta aaaaaagaaa
     8161 aacctaaaaa tgataaaaag cttaagttct catataatga acaaagggag tttgaaaata
     8221 ttgactctga tatagaaaaa ctcgaatcta aaattgcaac acttgatgaa agtactgcaa
     8281 aattttcaac tgattttact aaacttcaag aaattttaga tgagaaatct aaactagaaa
     8341 aagaacttga atacaaatat gaaagatggg aatatttaaa taatctagct gatgaaatag
     8401 ctaataataa gtaatattaa aataatctta agtttaataa gttttgcttt aaatctgtaa
     8461 tttataaata ttttaaagga agtgtctcaa aatgaacttt ttagttttat gagacactct
     8521 tttttatgag aaatcaaata tattttcatc atttcaacaa tacaaaagag gcttatctct
     8581 attttgagac agcctctttc ataattacct tctaatttat atgctctaat atatctataa
     8641 taattcttat tatatctata tatccagtct ttcaatataa tgacaattaa cttcaatatg
     8701 atttaaaaat actaacaacc tttcataaag tggatgagat tcttctccat atttagcttc
     8761 taggttcttg cctatttctc taacagtcct ttgaccatca atttgtaaaa aaacaaaact
     8821 ggaatattca tctagttcta tttttttgta tttaggtatt ctagctttta actttctaaa
     8881 aaacttttga attttatggt cctgtttttc aagtatagta acaactctat tttcagctac
     8941 ttcatattca agtccatcag taattttaaa tacaatatct aaaacatcat tactcttgct
     9001 cattattttt tactttagat aaaacaatag gtaatgctgt aagcacaact aaaactacta
     9061 ataaagcaat tgccattgaa tttgttgcag caaatccact tggtaccttt ggagttacaa
     9121 ctccagttac ttgtaatatt atacctatta aaccaatgat agaaccacca gcaacaagac
     9181 cagaagataa acttatacca ttagaaactt taacttcttt ttctttttca gttttagata
     9241 ctaattcaac aaataaacga attaatgctc caactaatat tatagaagtt gttgcaattg
     9301 gtaaatagaa tcctatagct atagtcatta ttggtaaatt taatgcatat agaactattg
     9361 ccataaatac tccaacaata atcataaccc aaggtaaact acctgacata ataccagaag
     9421 ttaatgttga cattaagttt gcttgtggta atgcaaattg tacattatca ccagttgctc
     9481 taagttggtc agaaagtaat aaaattactc ctacaacaac tataacacca actatacttg
     9541 caattgtaaa gtaacgttgc atttcgtttt tgctaccacc aataatataa gttacttttt
     9601 gagattgagt ataacctcca gctatagcaa tagcaacaac tataaatgaa ccaaataaaa
     9661 gtaatgattt attagcttct aaatcagtcc atcccattat aacaaacact aatgttacaa
     9721 taactagaga agctatagtc ataccagaaa ctggaagatt tgatgttccg attgttcctg
     9781 ttaaacgacc agctactatg acaaataata aagataataa aagagatatg atagctccaa
     9841 taattgccat aacaatgtta ccagaaatta agaatgctgc taaaaatcca ataactactc
     9901 cacctaataa aagtatcatt tgtatagatg aaccttcttc tccttctcca gcacttgatt
     9961 tagctttaag tgtttctttt atagaagcga ttatagttgg tataagtttt attgcaccaa
    10021 taatacctcc acaaagcatc ataccagcac ctatatattt tacataacta ctagatattg
    10081 ctccaacatc catttggtta agaggcattg caggattatt ccataccata gctccatctt
    10141 tagccatatc tgtaaagtat cctattaaag gtgctatacc aaagtttgat aaaatacttc
    10201 ctgcaaacat tgttaaagaa acttcaagcc ctactataaa tccaattcct aaaagtagag
    10261 gatttacttc aatatcaaat ttccatttgt agaatttgct tcctacaaga ctcattacat
    10321 tgtttgctac atttaagaat gaaccagtta atacagtaat aaatccactt attacaaatc
    10381 caattcccat gtacttaatt gaatctccac cagcctctga agcaacaagt gtttcagata
    10441 tagccattga ttctgggtac ataagtttac catgttcttc aactattaga tagttgtgaa
    10501 ctatagcagc aattcctata ccaaataaaa ctccaccaac tccaacagct aagccttcaa
    10561 agaatgtaat ttggcttcca attaatataa ctgctggtaa tacgaaaata aatccacttg
    10621 caacagattc tccaccactt gacatacctt ggattaagtt tttaccaaga ataccctttg
    10681 aacgagcaaa tgctccaaca aaagcagaac ctattatagc tcctggaata cctgcggcaa
    10741 ctgtaagacc ggctttcatt ccagagtaag ttgtagatgc ggcaaaaatg gcagctaaaa
    10801 taataccgat aatcagtaca gctacatttc caccggttct ggacttatct gtgatatatg
    10861 gaacgtaatc ttttccacta acctcaccat aagcaccctt aggtaatttt ttgttcataa
    10921 aattttcacc ccttttcaat ttgtatatat tataacatat atacatataa aacttaactt
    10981 tttattattt tacaaatagc gagtcaaaac tactaaaatt taaccctata ttattaattt
    11041 ctacttttag atatttttat gctaattttc tttttatttt tcaattctac taaaaactat
    11101 taagatataa aataaaaaaa ttttttaatg taaaattaat tttgtaaaaa aaatgcttta
    11161 aaacacggaa aagtataaaa aatacaataa tcattcttat aaataaattt tcaccatagt
    11221 aaatatatac agaaaattct tgtttttatg caaattgatt cttttataaa aataagtgtc
    11281 ataaagaaat gtctgaaatc actttatgac acctatcata ataaaaaatt attctggtaa
    11341 tgctctttta tttacttttc cgattggtgt aactggtatc ttatctatcc tcattattat
    11401 tgttggaatc atatatggag gtagatgctc atttaaatat tctctaatag ttctttcatt
    11461 tatcttacca ctatcttcct catcaattac ataatatgca catatataca tatctccatc
    11521 atcatttgtt ctcgatatac aaacaacact tttaatttct gtaatattat ttattatatt
    11581 ttcaatctca tatagctcta tcctaaactc atcaattttt acctgattgt ccatccttcc
    11641 aatataatct aaatttccat catctaacca tcttactaaa tcaccagttc tatacattaa
    11701 cttattatgt tcacaagttt tgtaaggatt ctctacaaat acttgctctg ttagcccttt
    11761 tttatttaaa tatcctcttg atatttgaat gcctgataga catagctctc ctggtgctcc
    11821 aattggacat agtttgttct ttttatctaa tacatatgca taagtattaa atataggttt
    11881 tcctattggt atttttccat attctctgtc cacatgaaaa gaagtcgata caacagtaaa
    11941 ttcggttggt ccatactcat tatatagttc ataattacgc tttctatatc cttttaatct
    12001 ttctcctccg gtctgtacaa ctctaagtga attattattg caattttgta taaataacct
    12061 tgcaaatgct gttggcaatg ctagtatagt tatgtcattt tcctcacagt attcatttac
    12121 tgtatattca tcaaatttag cttcttcagg cataatatct attgatgcac ctacaagcaa
    12181 ataagggaat agttgtttca ttacagcatc aaaactgaaa cttaagtata taccacatat
    12241 atcattttct gtagcgctta tagattttac gctataataa cacatattta ctaaactctt
    12301 atgttctata gctatggtct ttggcctacc agttgaccct gaagtagata tcgtatatgc
    12361 acaatttgat gatgttattt tttcagttaa ttcatatcca agcttaaaca tttcttcatc
    12421 atttatatcg agtactatgg tattctcatt tacaacctta cttttgagtt catctgtagt
    12481 tatcactact tttgcattac tatcttcaag aaggtaattc aatctttctt ctggattact
    12541 tgtgtctatt ggaaaaaatg ctccccctga tttcataatc cctattgctg taataattat
    12601 gttaatatct cttggtaaca ttacagctac tatgtcttca ctttccactc ctatatgatt
    12661 taaataacct gcaagtttat cagttacttc atccaattct ttatatgtta actttctatt
    12721 ttcatcccta gcagctatat tactaggtgt taccttaact tgctccctaa ataaatcaac
    12781 ataactttta tcatatggta tttcatattg attattatta aagtcttcca ataatacttt
    12841 ttcttcttcc ttacatacaa attctaaatc ttctattaaa atatcattat cttctattat
    12901 ttgctctatt atataatcaa attgatttaa aattcttcta atagtttctc ttttatatat
    12961 agatggttta tactttattt ttattttaag tgccctctta gtatatacta ttacagataa
    13021 atcatatcct tttttttcta tgtcataatc atatgataaa tcaaaacttt tagctataaa
    13081 tattgtgttt ataagcttag tatttagatg atttttattc tctatatctg ataacaaata
    13141 gtatccatac tctaaagacc tgctccactg cctttttagc ttccttacaa catctataaa
    13201 tttttcactt tgtctaactc ttactcttat tggaattata ttcattgttt taccaaaaac
    13261 tacatcatct tcatatatat acttttgaag tataaggcca tatatagttt ctatgataga
    13321 gtctatgcta atattgttca ctttagaata ttgaattaac ctttcatgtt tctcaatatt
    13381 catcttaaaa acctcatcta ctacttcatc agaacaatct ttataaaaaa aatcatttgg
    13441 aatttttgct atattcgtat aatcatctaa caaatccatc caataattca tagctcgttc
    13501 taaattaact tcatacatat caaaattccc ccttactact aagtctatag aaaaaattct
    13561 tttggaattt tattttcaca at
//
LOCUS       DACULQ010000108        13426 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.108, whole genome shotgun sequence.
ACCESSION   DACULQ010000108 DACULQ010000000
VERSION     DACULQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.108"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            217..918
                     /locus_tag="JA154_12440"
     CDS             217..918
                     /locus_tag="JA154_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HAT7996656.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1203..2006
                     /locus_tag="JA154_12445"
     CDS             1203..2006
                     /locus_tag="JA154_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HAT7996657.1"
                     /translation="MKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKDTLGEN
                     VIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYHCKKYI
                     FSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEEIRSLS
                     KTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRMHGDIA
                     RIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2061..3209
                     /locus_tag="JA154_12450"
     CDS             2061..3209
                     /locus_tag="JA154_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAT7996658.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3345..4733
                     /locus_tag="JA154_12455"
     CDS             3345..4733
                     /locus_tag="JA154_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAT7996659.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4726..5460
                     /locus_tag="JA154_12460"
     CDS             4726..5460
                     /locus_tag="JA154_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996660.1"
                     /translation="MVNIILNWRFYFMKEKILILEDEIGIRSFVSINLKREGYEIVEA
                     GTGREAIEKMTTEKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDK
                     IEGFISGADDYIIKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKL
                     FKNGKEIELTPTEFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRN
                     KIEDDPSKPKYIQTIWGYGYCFRKEE"
     gene            5463..6896
                     /locus_tag="JA154_12465"
     CDS             5463..6896
                     /locus_tag="JA154_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996661.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6918..7655
                     /locus_tag="JA154_12470"
     CDS             6918..7655
                     /locus_tag="JA154_12470"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088290.1"
                     /note="mediates pseudouridylation (positions 38, 39, 40)
                     at the tRNA anticodon region which contributes to the
                     structural stability; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAT7996662.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7766..7973
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8226..9425
                     /locus_tag="JA154_12475"
     CDS             8226..9425
                     /locus_tag="JA154_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAT7996663.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9672..10718
                     /locus_tag="JA154_12480"
     CDS             9672..10718
                     /locus_tag="JA154_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996664.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGEYLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10826..11989
                     /locus_tag="JA154_12485"
     CDS             10826..11989
                     /locus_tag="JA154_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7996665.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12078..12458)
                     /locus_tag="JA154_12490"
     CDS             complement(12078..12458)
                     /locus_tag="JA154_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996666.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12461..13120)
                     /locus_tag="JA154_12495"
     CDS             complement(12461..13120)
                     /locus_tag="JA154_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAT7996667.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13226..>13426)
                     /locus_tag="JA154_12500"
     CDS             complement(13226..>13426)
                     /locus_tag="JA154_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAT7996668.1"
                     /translation="VNNSKIINFKNLSSSFKLIKLNEFEEYPDFILKLGDKKSFVESV
                     IKINKSNRKSIKNLILKLSKKP"
BASE COUNT         5390 a         1328 c         2302 g         4406 t
ORIGIN      
        1 tttgttttgt gctatcatat ttatataagg gaattgtcat aaaaaatatc ttgttcatac
       61 agttttatat agaaaaaaga aatgaatctt ttctttatat aagtatattt tgtaaattgc
      121 ctctatattt atagtttaaa taatagtggt attaaaaatc tgacaacaaa aataaaactt
      181 tcaaaaaatt aaatctaaaa agaataggag ataaatatga gtattgaaga tttatatgat
      241 tttgaatgtg ttcctttgaa caatgagatt gatattgatg ttttatttga aaagatgata
      301 gaagatggaa aagaaaaaga atatacacca attatagtca ttgatgaaaa agtagggcta
      361 ttaagaaaaa atatagatat gattactaaa gagtatggtt ctcttgaaaa ctatagagag
      421 tattgtttaa caaaatataa tgaaattgat gtaaatcaat tttttgattt taggaagagt
      481 gagatttcag aattattaaa ggagctaatt gatttagaag atgattttta tgattataaa
      541 cattttagac ccattatgaa tatagatata tttcatgaat ataacaaagt ttacatagct
      601 aaaattccaa caattaaacc atatgaagta tttgcataca tacctattgg cggatttaat
      661 gattgtccta gagatgaaga acatatagct attgcaaaat attggtatga aaagcatgga
      721 atctttccaa tcgcaattgg atgtgataca gttcaatatt ctgtaaaaaa tttaactaaa
      781 gatggtaaaa aatttgatga cttatgtatt gaattagtat tttattgtga ggatattatt
      841 atacaagggt atgaaacctt aaaggctctt aaggatgtat taaaacgctc tacaatagtg
      901 cttttttggt gggattgata ttctatcatt tagatagtat taaattgtta gtcaccaaga
      961 atccacttgc tttagttata gggagtgtgt caaactaggt ttttatacac agtgtagcat
     1021 agtaatatgt ttaaattaca aaggttaaaa aaatacttta atatttttaa tattataatt
     1081 aatagattta aggtaattat aatttataaa ataaatactt tatatgttat aatcattaag
     1141 aatgtaaaaa aataatatat agttttatag ttcatatttt tccagatatg gaggtaaatt
     1201 ttgtgaagga aaaagaaaaa ttagataaat taaaaaaaat gttacttgaa ttaggcagtg
     1261 ttgtagttgc ttattctggt ggagtagata gtaatttttt gcttaaggtc gcaaaagata
     1321 cattaggtga aaatgttata gctgtgacta ttcatgcaat gatgcattca agtagggaaa
     1381 ttgaagaggc taaacaatat actcaaaatt ttggtgttaa acatatcata ttaaatatag
     1441 aaaactttga tttaaaagaa tttaaagaaa atggtgttga tagatgttat cattgtaaaa
     1501 aatatatatt ctctaaaata aaagaggtag caaaagaaca taatataaag tacatagtag
     1561 atggaacaaa tatagatgat ttaggagact atagacctgg gcttaaggca ttgagtgaat
     1621 taggagtaat tagtccatta aaagatagta gtttaaaaaa agaagaaatt aggtcgcttt
     1681 caaaaacatt aggattaaat acttttaata aaccatcatt tgcatgtctt gcgagtagga
     1741 taccttatgg agttgaaata acagatgaaa atttaagaat agtagaaaag agtgaagaat
     1801 atctatccaa tttaggtttt tctcaattta gagtgagaat gcatggagat atagctagaa
     1861 tagaagttgg acaggaagag ctaggtaaat tttttgaaaa taacaatttt aataaagttg
     1921 atactaaatt aaaaatattt ggctttaaat atgttacact agatatgtca ggatataaaa
     1981 tgggaagcat gaatttaaat gtgtagatta ttttaaaaat aaataagttt tatagttaat
     2041 ataaggaaac aggaggtttt atgaaaaatt atacgttaat atcgccatgt ttttttggaa
     2101 tggaaaaaat gctggctaga gagattacta atttaggata tgaaataata aaaacagaag
     2161 atggaagaat aacatataaa actgatgagt ttggaatagc aaagtcaaac atgtggttga
     2221 gatgtgctga aagagtacat cttaaaattg ctgaatttga ggcaaaaagc tttgatgaat
     2281 tatttgaaaa tacaaaaaga ataaactggt ctagatatat accttatgga gctcaatttc
     2341 ctatatcaaa agcttcctct ataaaatcaa aattatacag tacaccagat gtacaagcca
     2401 tagtaaaaaa ggctatagta gaaagcttaa agaaaagtta tttggaggat ggtttgctta
     2461 aagaagataa ggagaaatac cctatttttg tatttataca caaagataaa gtaaccattt
     2521 ctatagatac aacaggagat gctttgcata agagaggata tagagaaaag gcaaataaag
     2581 ctccaataag agagacttta gctgctgggc ttatttattt aactccctgg aaagcaggta
     2641 gagttttagt agaccctatg tgtggttctg gtactatatt gattgaagct gctatgattg
     2701 gtataaatat ggcacctggc ttaaatagag agtttatatc agaaaagtgg agaactcttg
     2761 ataagaaaat atggtgggat gtaagaaaag atgcttttaa taagatagat aatgaatcta
     2821 aatttaagat ttatggatat gatatagatg aagaatctat agatatagct agagaaaatg
     2881 ctgaaatagc aggtgtagat gaatatatag agtttaatgt aggagatgca actcaattta
     2941 aaagtgaaga tgagtttgga tttataatca ctaatcctcc ttacggagaa agattagaag
     3001 ataaagacag tgttaaacag ttgtataagg agcttggtta tgcatttaga aaattaaaaa
     3061 attggtcgta ttatttgata acttcttatg aagactttga atatgagttt gggcaaaaag
     3121 cagataaaaa aagaaaattg tataatggta tgttaaaaac taactttttc caatatccgg
     3181 gtcctaagcc tcctagaaat aataaataag ttttacatga tttatttgta aaatataaaa
     3241 ttttattaaa ataagtaaaa cattatgatg ccctgaaacg tctaattatt gtgaggagat
     3301 ttttgtgaac aaatttaaag atttatagga ttaggaggag ttaaatgaaa gtgttaaagc
     3361 ttattactgt agtagccctg atgatgttat ttgttactgg atgtaatata aaaactgaat
     3421 ctccagaaca attagcgaag atacctgact atgatgatac aaaaaaagta ttgtatgatg
     3481 gtattgacca gttactaaaa ccagattcat ctatgatatt gccttctaat acaaaagaag
     3541 taggaaaaat aaataaggta gatttaaaca gcgatggaat tgatgaatta gtagtatttg
     3601 aacaaaaaga agatttaagt aataatgtga gtcaggtagg gtttgtaacc ttgagttaca
     3661 atggagaaaa atacgtactt ggagaccatt ttttagaaaa tggcgaatca atagaatatg
     3721 ctaattttta tgatttggac tcagatggat ataaggaagt tatcttattg gttaaatcca
     3781 aagacaaaac gaatatgcat atatataaag tagtagataa tgaaataaca aaaatatatg
     3841 acttggatgc atcatggctt caaaataaag aagattttaa tgacatgaaa gtaaaaattg
     3901 gatatataga tggagatgac aagttagaca tattgatgct tcatttgaat aataagacta
     3961 atgaaatgtt tgcaagtgtt gcaaattttg atgggaaaat gaaaatcaaa gattctgtta
     4021 agtttgaaaa tgtaaaaaac ttaagtgaat tatatataac attaggtaat gttgcttcat
     4081 ctgttggaaa ttcttctgca gctataaaag gtattgtact tgatattcct atattgaaag
     4141 ataataacta tattacgcag atactttata tgaaagatgg gaaaattaac aaagctttta
     4201 gtgattatga taaaactatt actaaatctt attatatacc tgtagatgat attgacaaag
     4261 ataagattat agaaatacct atagttgcag gcagtacagg taataataaa aatacttata
     4321 gttctaaatc atctgcaact ataagttggt atagatggaa tggaaaagaa ggtgctgact
     4381 caagtttaat atttacaagt cagatatatt ataattataa atataatttt aaattattta
     4441 ttccaaataa tttatttgat aaaatacttg ttgaacagga atttgttgga gaaaaagcag
     4501 tatttaaatt ttactatttt gactattcag accgtaagaa tttatttaat atagtagtag
     4561 agagtaaaaa taaacttgaa gatagaaaaa atacaggtac tcaaaattct attgtacttc
     4621 aagagagcga tgaatatact ttcttactag ttgtaaacaa tgcaaatgaa atggataagc
     4681 tagatatgac tatagacgca ttaaaagaat atttctcatt aatatatggt taatattata
     4741 ctcaattgga ggttttattt tatgaaggaa aagatactta tactagaaga tgaaattggt
     4801 attagaagtt ttgttagtat aaacttaaaa agggaaggct atgaaatagt tgaggcaggt
     4861 actggaagag aagctataga aaaaatgaca acagaaaagg atattacaat agctctttta
     4921 gatgtaatgc ttccagatat aagtggtata gaagtatgta aatttataag ggagaatttt
     4981 gaccaagtag gtataataat gcttactgct aaagctcaag aagatgataa aatagaagga
     5041 tttatctcag gagcagatga ttacatcata aaaccattta gtataaaaga gcttttagtc
     5101 agagtttctg ctcttcttag aagagtagca aaggatgatt caagtgttaa gtcgagtgag
     5161 atagtttctc caccatttat acttgatata gacaaaagaa aattatttaa aaatggaaag
     5221 gaaattgagc ttacaccaac tgaattttca atagtgaaat atctaatatc aaatgctaag
     5281 cagtcgttaa gtagagacca gatattagac gaagtatggg gaactaacta tttatatgat
     5341 tttaaaatag tagatgtaaa tataagaagg ataagaaata aaatagaaga tgacccatca
     5401 aaaccaaaat atatacaaac tatttggggt tatggatact gttttagaaa ggaagaataa
     5461 tagtgttaca ttttgaaggt ctgagaaaaa aagtaataaa aaactatttt ataataatta
     5521 ttataatggt tactctgttt gaaggattgt tcatgtttta catacaaaac tactattatg
     5581 attctgtcaa acagctactt gaatctgaaa taaaatatgc tgatgaatat aatgctatta
     5641 caatggaaac tacaagtttt gagaaaaaag taaaaaatat atttgataaa caacccttaa
     5701 ctaaaaattc agaatttgga atttcaataa tagataaaga taaaaatatt atattagacc
     5761 aatatggttt taaaagtaaa gaaaaagcaa actatgaaga tgttaacaat gcattaaaag
     5821 atataaaaac taaaaattta acaccttata cttatagaat accagataca ggggagcatg
     5881 ttatgagtat ctccttacca cttaaggtaa ataatataat agaaggtgtt gttagatata
     5941 cagtatcatt agatgctatt gataatgcaa tattaaagca agctacatgg cttatattag
     6001 caggaatatt tatacttatt atagctatac taataagtct taaatttgct gagactctta
     6061 taaaaccatt acgagagcta aagaaatttg caaatgaatt ggctgtagga aattataata
     6121 taaaattaga aaagatgaag attgtagatg atgaaattgg tgatttagca cagacatttg
     6181 aacacatggc acatgagatt gacaaaagtg aaaaattaaa agaggaattt atatcatctg
     6241 tatctcatga actaaggact cctcttacat ctataaaggg gtggagtgaa accttaggtt
     6301 atgaaagtat aacaagagaa gaactagact taggtttagg cattatacaa gatgaaacag
     6361 aaagacttat aaaactagtt gaagaattat tagacttttc aaggctttct tcagacagga
     6421 taaaacttca tgttgatata gttgatgttg aaggtttaat tgtaggtgtt gtaaatcaac
     6481 ttaaagttaa agcagctgaa aaagatatat cacttttatt tgaatttgaa aatgaattta
     6541 tagagaacat tcaaggagat aaaaatagat taagacaggt tctcataaat ttaatacaaa
     6601 attcatttaa atttacgagt caaggtggtt acataaaagt tgtagcttct caagatgaag
     6661 agataacaac catatcagta gaggataatg gttcaggtat agaaaagcaa aatttaaata
     6721 aagtgttaga taaatttttc caagaagatt ataataaggc tggtagtgga ttaggactag
     6781 caattagtaa tgaaattgta aaacttcatg gtggaagaat gaaaatagaa agtgaaaaga
     6841 atgttggaac aaaaataact tttaatataa aaaataaatt tgctaaacaa gcataaattc
     6901 ttagaaagag gattaagatg agaaacataa aaatgatagt agcatacgat ggttcaagat
     6961 ataaaggata tcaaaaatta ggtgacaata atatgactat tcaagaaaaa cttgaaaatg
     7021 ttttaagtaa aatgactaat gaaacagtgg agataatagg ttctggaaga actgatatgg
     7081 gagctcatgc aagaggacaa gtggtaaatt ttagaactaa ttgtatggat tctttggata
     7141 aaattcaaaa atatttatat gaatatttac cagaagatat agttgtaaaa actgttgaag
     7201 aagttgatga aagatttcat tcaagataca atgtaaaatc aaagacttat atgtataaaa
     7261 ttgacaataa taagtatcat aatcctttta taagaaaata tgctactcat gtaagtaaaa
     7321 aattagattt agatagaatg agaaaagcaa gtgagtattt agtaggagaa catgatttta
     7381 ctagttttgc ttcttctaaa tctaagaaaa aatcaaatgt aagagaaatt tattctataa
     7441 atataaaaga agatgataat gttatagaaa tatatgtaga aggaaatgga tttttgtata
     7501 acatggtaag gataatagta ggtgctttga tagatgttgg tctaaaaaga agagctccac
     7561 aagatattaa gtatatgctt gaatcaaaag atagatgtca atcatcagat actgcaccag
     7621 ctaaaggact atgtctttgg aaagtaacat attaatcaat tatataaaac tctctttaaa
     7681 ataaaaataa aataaattat atagttgaca taaatggaaa aataaactat tatatatttt
     7741 ataagagaga tataaaactt tatatttaaa tcctgtgaga aagagagtaa cttctatgtg
     7801 ttttatagag agctgaggat ggtggaagct tggcaaataa gtattagtga aaagaacttt
     7861 ggaggatact aattgaattt agtatgttag taggtaatct cgccttaaga gaaagagtgg
     7921 ataagtttat cttatcaatt agagtggtaa cgcggatata tttcgtctct tggtttttcc
     7981 aagaggcgtt ttttaatata tttaattatt agtgtttatc tggtatgttg aacatttact
     8041 aattgattag ctaagtttat caataagagt ggtaccgcgg atatttcgcc tcttagtatt
     8101 ttaaaatact aaggggtttt tttagtatta aaatgatttt atgtgagtct aaattctcag
     8161 agtcattcaa tattaagaat ttctataaaa taaaaattat aatttaagat tgggaggaat
     8221 taaaaatgaa ggaaaaagta gtattagcat attcaggagg acttgacaca tcaatcataa
     8281 taccttggtt aaaggaaaat tatgaagata tagatgttat agctgtttgt ggaaatgtag
     8341 gtcaggaaga taaaatggaa gatgtatatg aaaaagcttt acaaagtgga gcatcaaagg
     8401 catatgtgga tgatataagt gaagaatttg taactgaaac tatatttaaa gctgtaaaag
     8461 cagaggctaa atatgaagga aaatacttat taggaacttc tttagcaaga cctataatag
     8521 ctaaaaaatt agttgaagtt gctcataaag aaggtgcaaa atatatatgc catggatgta
     8581 caggaaaagg taatgaccaa gttagatttg aagctacaat tgcagctctt gacccaacta
     8641 taaaagttat tgcaccttgg agaatttggg atataaaatc aagagaagat gctatagact
     8701 atgctgaaaa acataatata aaggtaacag caactaaagc taaaatatat tcagtagatg
     8761 ccaacctttg gcatgtttca acagaaggtg gagatataga gcacctagaa aatgaacata
     8821 aaaaagatgt ttataaacaa tgtgtagacc cagaagatgc atgtgatgta gctgaatatg
     8881 tagaagttta ctttgaaaaa ggtgtaccta aaaaggtaaa tggcgaagaa ttatctccag
     8941 ttgcactgat tcataagtta aatgagttgg gttgtaaaca tggaattggt gttatagata
     9001 tagttgagaa tagacttgtt ggtatgaaat caagaggtat atatgagact ccaggaggaa
     9061 caatccttta tgaagcacat aatattttag aaagtgctac tttagacaaa gatacattac
     9121 atttcaaaca aatggtatct tataaatacg gagaacttat ttacaatgga ctatggtatt
     9181 gtaaattaag agaatctata gatgcattta tggaacaaac tcaagataat gttacaggaa
     9241 cagtaaaagt taaactttac aaaggaaata taaaacctgc tggaatattt actgaaaatg
     9301 ctctttatga tgaaggaata tcttcttttg gaaatagtga actttatgac cacaaagatg
     9361 cagaaggatt tattaattta ttcactttac cattaaaaat cagagctatg aaagctggaa
     9421 aataataaaa taaattattt aagagtgtat aaaaacgatt tttaggtttt tatatgctct
     9481 ttttttattt gaatttatta aattatacag taaatagtat aaaaaaatat attatagttc
     9541 caaatggctt aataatgtga aaatataaga cctaaaatat aaattttata aatacaatag
     9601 ttattgactc tctcattgta agagggttta tactgaatct aatgaaaatt tatagtgagg
     9661 agatagatta tatgagaaaa ataattgaaa ttaaacatca ttcacatgat tatgagtgta
     9721 tgtggaatgg tatagaagat ttatatataa ataaaaccgg tgaatacctt ccaaacgatt
     9781 ttttcttttt actgtcaggt tttggttcat tttgttatat gaaaacaaat aaagctgatt
     9841 tgaaaagaat ggtagctttg ggagatggaa ggacaaaaca gatgtataaa tttttagcac
     9901 caattgttgg atttgaatac aaacatcatg agtttaaaaa gtatgagcaa gcattaaaga
     9961 aagcaaaatc tgaaattgat gatggatatc cagttatttt aggagcttta gatatgtatt
    10021 atctaccata ttataagaag ctatatcata aagaacatat tccttttcat tatatattaa
    10081 tggtaggata tgatgatgaa ggaatatatc tgtatgattg tggtagaaca gaattattac
    10141 atctttctta tgataaatta cgtgaaagca tgaattgcaa ttcccctgga ttaagcaaag
    10201 aaaatactat ttgcacaata agaatgaata cttcaaagaa taaatataac attgctagag
    10261 aagctattgc tataaagcgt gatatgtttt taaatccacc taaaggattt ttgggatata
    10321 aaggttttga aaagtttatt aaagagttgc cacagtggaa cactgaattg gataaggatg
    10381 attatgataa gattttgacc aatatggtta tgttttttgg aacagtacca acaattccaa
    10441 atgcaatcaa aggaattaaa gaaccagata ctgttcaatt taaaggtgga tttgataaaa
    10501 tgagtagaat gctcaacagt attggggagg aatacaaaaa caactctttt atacaagtag
    10561 ctgatatttt tgataaaact gctattataa ttgagaaaat atccaatatt ataatagatt
    10621 acttaactca aacatgtgat gatacagaac aacttccgtt attgttctct gaggtcttag
    10681 agcatatgaa aacaggatat ttaatcttgg atgtataaaa aatatattgt tgaccctaac
    10741 attacgtaat agtgtaatat gaaaatatag cttaaatata ttagtaggta ttgatggctg
    10801 taaaattaaa atttgaggtg tggatatgag aacagtgaaa caagtttctg atttaactgg
    10861 aataagtgtg agggcattac attattatga tgaaataggt cttttaaaac caaataaaat
    10921 tacagatgca ggatatagac tttatgatga tgaatctatt aaaactcttc aacaaatttt
    10981 attttttaag gagattgata ttcctttaag agaggttaag gaaataatgt caagccaata
    11041 ttttgataag gttgaagctc taaaaaatca aaaaaaatta cttattttaa aacgaaagag
    11101 actggatgaa ttgatagagc ttataaatca aactctaaga ggagaaggaa atattgattt
    11161 taaagagttt gatatgagcg agtattttaa tgttttagaa gaatttaaaa gagagcatag
    11221 gaataaggta attaaaattt atggtagtgt agaaaaatac aatgaatata tagaaagagt
    11281 taagtctaat gaagagaaaa ttgctaaaat ggctataaaa caatatggaa ctattgagaa
    11341 gtttgctaaa gctataaaaa caaattttag cagtgatata ttgaatttag gagaaaaatt
    11401 tgatagatat aaaaatgatt gtttaaaaga aaaacatcca aaattaaaag aattatatag
    11461 aaagcttgta gaagatttaa gtaggaatcc ttcttcaaca gaccttcaag aaatagcaaa
    11521 agcgataaca gatatatcta aaaaagatta tgaaattttc tctatggata ctggtgatga
    11581 caactggtat tatatggttc aaaattatct agtaaatcct atgtggatag aagaagtaga
    11641 taaaaagtat ggaagtggag caggaaagtt tattggacaa gtactaaaga cttatctaag
    11701 agatagaaaa ccaaagataa atacattgta tgaaaaactt gtagaagatt tgagtagaga
    11761 ttgttcttca agggaagttc aaagcattgt tgaagaaata gacaatgaaa tgaaaagaag
    11821 taatgaattt tataaaatag acaatggtga aagatacttt gattatatgt cagagcttta
    11881 tttacaagat tctaactata taaaagtaac tgataagaat tatggtgatg gtgcatctaa
    11941 gtttattgga gaagctttta agatttattt tgataataat aattgttgaa aatttggaag
    12001 taaaaaatat gtaagtttta taaaagcaca aaaataagga tgtagattag ctccatctat
    12061 atccttattt aaagttatta aaatatatag tttacatcaa acttaacact ttcaataata
    12121 tttcccaaat atgtttttat actttcaact tcttctaaag attttaattt attttcagta
    12181 taaattgctt tgtccagaat attaaacctt aatcctattt catcaaagta ctcatgattt
    12241 aaaactttgt accaatggtt tttttcttta ttccaactcg tagatattga atcagcttct
    12301 ttttgagctt tttcccaatc ttcattttct atagattttt ctaaaacaga tatattgtct
    12361 ttatatcctt ctgtaaaatc atatagttta ttgttaacgt aaaatccaaa taatacaaat
    12421 aagatagtcc atagtataac aaatgttgta gatttcaata ttaatcaccc tttttatttt
    12481 gaataaatat tttgtcattt tcatctaaaa cacaaataag aacatcttta aaagtttcta
    12541 tgtgattaga ttttaaaata cccattaacc agttagtatc tttttgagct atgtctagat
    12601 ttttatttat tattctacca tccagtataa gtgggatagg taaatgatta aaagctcttg
    12661 gaggagtact attgtagctt gatgctggta caacacttag attaccatca gtctccaaaa
    12721 tagcatattg tacatctttt aggttaaagt atccttgaat cctaagttgt tctaataatt
    12781 catctatact aattctttct tttttaagct ctttatatac aatttttcct ttatctatta
    12841 atacagaagg tttacctgat aaaaatgaac ttaattttct gctttttaat gataagaatg
    12901 aaattagagt ctgcatgatt acaagtccag atattgctgc aacaccattt atgattggta
    12961 tattattatt ttccattggg acagatgcaa cttctgcaat taatagtgtt ataactaaat
    13021 caaaacagtt catttcagct atttcacctt ttcccatgac tctaagtgca attagcactg
    13081 ttatgtataa tataatactt cttattaata ctactgtcat atctatcaat tactccttta
    13141 aaaaatattt acacttagtt agtatatcct atataaaaaa tttaattcaa agtataaaaa
    13201 accttaaata attggattca actatttaag gtttttttga taattttaat attaaatttt
    13261 ttatggattt tctgttactt ttatttattt taataacaga ttcaacaaaa ctctttttat
    13321 ctcctaattt taagataaaa tcaggatatt cttcaaattc attgagtttt attaatttga
    13381 aagaagaaga tagattttta aagttaataa ttttagagtt attcac
//
LOCUS       DACULQ010000109        13338 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.109, whole genome shotgun sequence.
ACCESSION   DACULQ010000109 DACULQ010000000
VERSION     DACULQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.109"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(171..914)
                     /locus_tag="JA154_12505"
     CDS             complement(171..914)
                     /locus_tag="JA154_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAT7996669.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            1080..3647
                     /locus_tag="JA154_12510"
     CDS             1080..3647
                     /locus_tag="JA154_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAT7996670.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            3877..4281
                     /locus_tag="JA154_12515"
     CDS             3877..4281
                     /locus_tag="JA154_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAT7996671.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4305..4802
                     /locus_tag="JA154_12520"
     CDS             4305..4802
                     /locus_tag="JA154_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIB"
                     /protein_id="HAT7996672.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            4805..5206
                     /locus_tag="JA154_12525"
     CDS             4805..5206
                     /locus_tag="JA154_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HAT7996673.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5224..5712
                     /locus_tag="JA154_12530"
     CDS             5224..5712
                     /locus_tag="JA154_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter"
                     /protein_id="HAT7996674.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            5739..6494
                     /locus_tag="JA154_12535"
     CDS             5739..6494
                     /locus_tag="JA154_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT7996675.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6494..7357
                     /locus_tag="JA154_12540"
     CDS             6494..7357
                     /locus_tag="JA154_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAT7996676.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7404..7883
                     /locus_tag="JA154_12545"
     CDS             7404..7883
                     /locus_tag="JA154_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996677.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            7948..9255
                     /locus_tag="JA154_12550"
     CDS             7948..9255
                     /locus_tag="JA154_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HAT7996678.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVSGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIVHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            10215..11309
                     /locus_tag="JA154_12555"
     CDS             10215..11309
                     /locus_tag="JA154_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7996679.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            11314..11514
                     /locus_tag="JA154_12560"
     CDS             11314..11514
                     /locus_tag="JA154_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996680.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11514..12431
                     /locus_tag="JA154_12565"
     CDS             11514..12431
                     /locus_tag="JA154_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996681.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12428..13186
                     /locus_tag="JA154_12570"
     CDS             12428..13186
                     /locus_tag="JA154_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996682.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
BASE COUNT         5117 a         1392 c         2459 g         4370 t
ORIGIN      
        1 ttattaaaaa tgttataatt tctattgcta tcatagacca tatcaatcca tcaagttcat
       61 aaaagtaatt aagctttatt aaatagcttc tttattataa tttctttatt tcatataata
      121 taaatagtat taaaattttt atgggagtta aagtaagcat gtattattac ttagatattt
      181 gtataatcaa catctagtaa tttacctttt tggtgaaaat gattaataag ttttttagga
      241 taattcatta ttaattcatc tggaaaatta atttccttta agatattttc agaatactca
      301 tagttaccta tgtcaaaaca aatgtgagca tctgaagtca gtatgatttt agcaccatat
      361 tttttgcaaa gtgtagcaat atacttacag ttatccgagc taccatttct tgacacgcct
      421 ttaatagaac aattatttat ttctattaag atattctttt ctatagctaa tttaatgatt
      481 aattcatagt cgattgggta gtttgggttt ccaaggtgtc ctaatatttc aatattatca
      541 tgcttattta ttgcatttat taaagcttta gtattattct ctaatgtatt tggagaaaac
      601 acagcttcgt gaaatgacaa aataagataa tctaagcgag gaattacaaa aggctctagg
      661 tctatgtttc ctttaatatc tagtatattt gcttcacagc ctcttaaaat tataatgtta
      721 tttatgattc ttgggatatt ttgaatgttg tgaaagtacc atctgtgggg agaaccaggc
      781 atcttaggac catgttcaga tgtacctagt atcttaattc cattttcttt acaataatta
      841 atattttcta gtaaagtgct atagccatga ccactgacta tagagtgggt gtgtaaatct
      901 gataaaaaat tcatataaat tcctcctcta taaaagatga gggtaacgta atttatatta
      961 taaaacgaat aggaaagaaa atcaatagtt acataattag acaaaaatat taaaatattg
     1021 tagaattaag cttaataata aatgtttaac agtattactg cttatagggg aggttaacta
     1081 tgacacaaaa gaagctatca aggaaagaaa aagttttaat ttgcttgaaa aggttaacag
     1141 aagaacactt gaactttaat aatggcatta gatttggatt tgatgcagaa tatatagcta
     1201 ctgaagtagg tataaataga agtaatgcta gtaaggaatt aaatcaatta ataaaagaaa
     1261 gtattattat aaagataaag ggtaagcctg ttcagtactt atataaagaa agtattgaga
     1321 gtattttagg aaagaaacta aataaaggga tatttgataa tattaactct ataaacgaat
     1381 atagtgaaaa tgattctatc aaagatgagt atattttaga gagtataaaa aaagagaaca
     1441 atatttttaa tttagtagga tgtgatggaa gtttaaggtc ccaaatagaa caggctaaag
     1501 cagcagtaat atatccacct aaggggttga gtacattaat aataggacct acaggagttg
     1561 gtaaatctat atttgcagaa tatatgtata agtatagttt aactataaaa gaagatagtg
     1621 ataatgcacc atttatcata tttaactgtg ctgattattc tgataatcag caattattat
     1681 tagcgcagct ttttggatat gtaaaaggag catttactgg agctgataaa gataagtatg
     1741 gtattgtaca tgaagctaat aatggagtat tgtttttaga tgaagttcat agattatctg
     1801 cagagggaca agaaatgcta tttctgctta tggataaagg gatatataga aggttaggag
     1861 aggctaataa ggtacacgag tgtagagtta tgataattgc agccactaca gaagacccag
     1921 aaacagcaat gttggaaacg tttttaagaa gaatacctgt aactataaag attccgtctt
     1981 tagaggaaag aggctttcaa gagagaatga agcttatatg ttattttttt aaagaagaga
     2041 gtgttaggat tggtgttaag ttaaaggttt caaaagaagt tattaaggca tttattttat
     2101 ataagtgtaa gggaaacata ggccaattaa aaagcgatat acaattaatt tgtgcaagag
     2161 cttttttaga ttatatgacc tataaaaggg aatgtgtata tgtaaggctc tctcttctac
     2221 caaataatat tagagaatca ttatatggaa ataatagaaa agaggagttt gtacaaaatt
     2281 tcagttttat tggaaaagat gatattattt ttttaccaga agaaaaggat tttgaaagtg
     2341 aaaatttatt aatagaaaat aaaaaatatg atttagattt ttatggaatg ataaaagaaa
     2401 cttggaataa acttcaacaa gaaggtatta aagagtcaca aattagaagt gttatagatg
     2461 gagacataca gaagtattcc tataatttaa tgaatacttt tatatataat agtagtaatc
     2521 aaacagctta taacaacatt gttaatgatt caattgcaac tactgtaaaa tatattttac
     2581 aaaagcatga taaatggagc aaaagagaag gattagataa attaataaaa gctatagctt
     2641 tacatattca gaatttaatt gaaagaataa agattggaaa tatagtaaag catcctaata
     2701 aagatgaaat tatgaaggaa agagcatatg aatataacat agcaaaagaa atattatcta
     2761 acatgtcatt aatgtatgga gtagagtttc cagaagatga agcaattttt ttagcaacat
     2821 ttctttattt atcgtatgtg ggattaaatg aggagagtat agcaatatta gtcataatgc
     2881 atggtgaatc tacagcatct agcatggtga atgtagccaa tactttactg gattgtaacc
     2941 atgcagtggc aattaatatg ggattagaag atagagttca ggatatttta gtacaagctg
     3001 tagaaatagc caataaaatt gataaaggaa agggaatttt aattttaact gatatggggt
     3061 ctattttaac atttgctaaa gttattcgtg atgctacagg aaaagaagta aaagcaatag
     3121 atatggtaag tacacctatt gtaatagaag caactagaaa ggctctaact ccagaaatga
     3181 cattagagaa attatatttt aatattatag aatctataaa gaaacattat ggagatatgg
     3241 aagttacata tgctgaaaaa gataatggtg gtagatattt tgatagatta ctaattgata
     3301 atattagtaa aactttgact tttttaaatg gggaaaaggc ttattttata ttaaaagaag
     3361 taattaatag aattagtgaa cactattctt tagtaattca agatgaactt ttagtaaagt
     3421 ttatatttca ttgttcatgt atgattgaaa gagtaataat aaaagagtct ttagtttata
     3481 aatcatatga agaaagaatt agtagaaata aagaattata tttagttata aaagaaagtt
     3541 ttaagctagt agaagagaca ttcgatataa ttatttcaga tatggaatat gcaaatatat
     3601 tagatatatt tgaatcacaa tatgatacag ataaattcaa aagttgatac aaaatttaaa
     3661 aatagccaaa aggctatctt ttttttactt tttttgatac agatttgata caggttgaaa
     3721 aaatcttttg atacatatcg aaataataag tttttaaaaa aaattgagta aaaagttttg
     3781 attaaatcct gtaaaatagc catttgttat tgatacacat aagttggcac ggcaattgct
     3841 tatataaaaa gtgacaagaa aagtgaggtg aaattggtga taggaataat agttatgact
     3901 catgggagtt ttagtgaaga aattataaaa agctgtgaac ttattgcagg accagcagaa
     3961 agaacagcag caattaaact aaataggaat gataatattg aagacttaaa taaaaatttt
     4021 gtagaaaagt taaatgaatt agatgaaggt gacggagttt tagtattagc tgacctatta
     4081 ggaggttcgc caagtaatgt tgcaagttta aaccttaaaa aaggtggcaa gtttcatgca
     4141 cttactggag taaatcttcc aatgctactt gaagctttaa ttaatagaga gggaaaaagt
     4201 ttagaagagt tagcagaagc atgtattgaa gcaggacaaa ctggtataaa caatattaat
     4261 aaagttctta gctcaatgta atagaaaaag gagtttgatt aaaaatgtct ttagtaaaat
     4321 attgcagatg tgatgataga ttaatacatg gtcaagttat atataaatgg gttaagcatc
     4381 taggtgtaaa aaagatagta gtagtagatg atgaaacaac caatgatgtt attgctaaag
     4441 gtttgataaa aatggcagca ccaaagaata ttgatttatc aatattaact gtaagtgaat
     4501 caaggagata tttttataat aatcaagctg atgataacgt ttttgtatta attaaaaatt
     4561 tagatactgc aaatagaatg atagaagaag ggattaatat caaaaaactt attgttggaa
     4621 gaatacctac aggaatagga aaaaagaaaa tatctcaaaa tgtatacatt aataaaaaag
     4681 agtttctttt aatagatgaa tttattaaaa agaatattaa tgtatcaatt caaatggtac
     4741 ctgatgaaga agaagtagaa ttaaaccaaa atattaatcg ctataaagga gagtttattt
     4801 agcaatgaat aaatataagg taatcatact gtgccatgga acttggggaa aagtcttagt
     4861 agaagaaaca aataaaaact ttggattgtc aaatcaatat gaagttttat cactaagttc
     4921 tgaaaaagat gtttctgtat ttatgaaaga ggttgaagaa gtagtagata aagaagatgt
     4981 gaaaattata atatcagatt tatatggagg atctacatca agtgttgcaa tagctgttgc
     5041 tataagaaga ggaatagatg caataaatgg acttagctta cagacaattt taatcgtaga
     5101 tgaagaatta agcaaagaag ataaattaca tacattacca gaaagaatcg tcaaaaaaaa
     5161 taataacctt tgtgtggatt taattaaaga atttaaaaaa atataaatta tgaggtgaat
     5221 ataatggcaa aaataacatt agtaagagta gaccacagat taattcatgg gcaagttata
     5281 acaaaatggg taaaaatcgc tcaagctcaa aaaataataa tagttgatga cttcttaggt
     5341 caagatgaat ttatggctga catatataaa atggcagcac catcaggagt agaagtagtt
     5401 attttaacag cagaagatgc gggacaagca ttccaaaaca acactttagg agataaaaat
     5461 atatttattc tatttaaaaa tgttgatatg gcaaataagg cttataaagc tggacttaaa
     5521 tatgaaaaga ttcaattagg aggtattcca aatgaagcag gaaagaaaat ggtgtttaca
     5581 gctgtttcat taggaaatga ggatgtagaa caacttaatg aacttaatga aaatggggtt
     5641 gaaatagtcc tacaagttat accagaggaa tcatctatga catatgaaaa tgcgttaaag
     5701 aaatttaaat aaaaaatata aattaggagg aattataaat ggaatctgtt ttgatgttgg
     5761 caattgtaac aggattatgg tattggtttg cagcaggtct tgcagggtat acccttttct
     5821 caacactaaa gtctccttta tttataggtt ttagccttgg attactttgg ggtgatgtaa
     5881 caactggtat gattgttggt gcaagtatag aaatggttta tctaggaatg gtcgcagcag
     5941 ggggaaatat tccatcagat aaatgtctag ctgctcttat tgcaatacca gtagcacttc
     6001 aaactggtgt taatgcagaa gttgcagtat ctattgcggt acctttaggt gttataggag
     6061 tattagttaa taacttaaga agaactggta atgctgtatt ggttcataag gcagataagt
     6121 atgctgaaga aggtaataca aaaggcatct ggagatgtgc aacactttat tctttaatat
     6181 ttgggtttgt tttaaggttt ccaatagttt ttgtatgtaa cttttttgga gcagatttag
     6241 ttcaatcatt attagatgtt ataccacaat ggttaatgaa tggattaaca gtaatgggcg
     6301 gtatattacc tgcattaggt tttgcaacaa caatatttac tataggtaaa aataaattct
     6361 taccaatgtt tattatagga ttttttatgg ttcaatattt tgaaatatca attactgcag
     6421 ctgcaatatt tggtgtttgt atagctcttt taattacctt tatgaaagaa gacaaaaggg
     6481 taggtgaagt ataatgcaag aaataaaaaa taaagaagtt aaaaatggac aaggtccaac
     6541 tgaagttaat aagacaagag tacttacaaa gaaagatgta acaaaaacat atctaagatg
     6601 gtggtggaca gcagaattat ccaactcttt tgaaagaatg caagcattag cagtttgtgc
     6661 aagtttcact cctgctttag aaaagttata taagagaaaa gaagatttag tagatgcgtt
     6721 aaagagacac ttacaatttt ttaatactca agctatatgg ggaggattaa ttcacggtac
     6781 agttttagct atggaagaag agaaagctac tgaaggaaaa atcccaggag aagttatttc
     6841 aggtgttaag aatggtctta tgggaccttt ggctggaatt ggagatactt tagattttgg
     6901 tacatttcaa acaatattct tagctttagg tgcttcattt ggagcagaag gaagtgttat
     6961 aggtgcattt ttcccaataa tgtttagtat attactattt tgcgaaggtt attacttatt
     7021 tcatttagga tattctttag gtagagattc aattaagaaa attctatcag gtggtattgt
     7081 caataagata attgatggtg cttcaattct aggaatgttt atgatgggag cattatctgc
     7141 aactacagtt aagctttcaa ctccgctatc atttgatatt ggtggtaaag caattgttgt
     7201 tcaagataca ttaaatatga ttgctccagg tttattacca cttggtgttg tattcttcgt
     7261 atattgggga atgaaataca agaagtggac aataactaag ttattagtaa ttttggttgt
     7321 attagcattg gtagggtcat tcattggaat attctaattc acagtgatta aaatatagaa
     7381 tttaatttta ggaggagaaa gaaatgtcag aaaaaatgta tacagaaaag gaatatttag
     7441 aaggaattag aggagcaaca ggagaaagag cagtatggtt ctatcttctt atgaaagaag
     7501 ctgaaaaatt aggagctaat ccagatgata tatgtaaaga agcaatatat ggatttggta
     7561 aaatgagagg tcaaaaatat aacgtagcag atactccagg aaaaatggca gaaatgttat
     7621 ataacagtaa aggacaaaaa gtgtttgaaa tggaattagt agaaaataca gatgagaatg
     7681 gcgttttaaa attccatcac tgtccattag atgctgcttg gaaagaatat ggattaacta
     7741 aagaagaaag aaaagaaatt tgtagattag cttgttatgg ggattatgga agagttgact
     7801 gtgcacaagg tgttaaatta gattttgctc aaaaatgtgc tcatgatgat gaagtttgtg
     7861 aattagtatt tacaaaaaaa taatttttaa tgctaaaagc ctgttcttta aggacaggct
     7921 ttaaaaagat agaaagtagg ttgatttatg gatataagca gaagtgtaga taatagttta
     7981 aatagaacca tagaatttct aaaagaattg ataaaaattg attcacaaca aggtgaacct
     8041 atttcacaat gtccttttgg tataggacct aaaaaatctt tagataaaac tttagattac
     8101 tgtgctagtt taggttttag tgttaaaaat attgataatt atattggata tgcagaaatt
     8161 ggagagggag aggaattaat agggattcca atgcatttag atatagttcc accaggagag
     8221 gggtggagtg tagacccatt ttcgggagca gtaattgaca atattattta tggaagaggt
     8281 gtcatagata ataagggtgc tgtatctatg cttatacatg tattgaaaaa catagaggat
     8341 atgtatccta caattaataa aagaataaga ttaatttttg gtacaaatga ggagacaggt
     8401 atgaaatgta tcaaatatta cttagataaa ggtgaagaaa ttccaagtat gggatttact
     8461 ccagatgcta tgtatccagt ggttaatgga gaaaaaggaa gggttcatat aaggatagaa
     8521 aaagaaatta aaattgacaa aagtaaacct tacataatag taagtggagg aactaaagaa
     8581 aatgttgttc catctcattg tactgcaaaa attattaatg gaattatttc agagttaact
     8641 actaaaggag tagctgtaca tgcaagtaat cctgaaaagg gagaaaatgc tatatctaaa
     8701 atggtaataa agatagttga ggataatatg gattttcagc atagagaaga tatagaatta
     8761 gtttcaaaat acctttgtag tgattattat ggagatgcat tggggattaa tcaatatgat
     8821 gaggtattca aaaacactac cttaaattta ggaatattaa aagtaaatga ggaaaaaatc
     8881 gtatgtgagt tagatataag gtatggtaaa aatatagttt taaacaatat aatagataga
     8941 ttcaaaaagg ttttttgtaa tggttggaaa atagaggtta ttgttcataa agacttacat
     9001 tatgtggatg agagtaattt agtacttaaa aaattattag aagcctatga agaagtaaca
     9061 gatgaaaatg gatatacaat tgctatgggt ggtggcacat atgcaagttg gtttaaagat
     9121 atggtggcgt ttggacctaa gttcttagcg tataaaactg gtggtcatgg agtagatgaa
     9181 agagtaccaa taaatcatat acgaaagaat atggagatat atactttggc tttaataaaa
     9241 ctattagaat tgtaaaagaa gaagagactt tatgtgcaaa tttacagata tcttaaaaag
     9301 agagataaaa cgagttcaac cttatttaaa tgttcaacac catcaaatgg ggctgtctca
     9361 aaatagagat aagcctctta cttattgttg aaatgatgaa aatatatttt acttcataca
     9421 aaaaagagtg ccttatgaag taaaaagtta attttgagac accctcattt gatgatgttg
     9481 aagagaaaat aagtatgaaa ttattaaaaa agatgatttt ataaaagtta aggttgaaaa
     9541 gaaaagttaa ttaaatggaa ttagataaat ggagaaggat gtatactatc taataaaata
     9601 acaaaagagt gttataaagt tggttatatg attagagaaa agtctatcga tatttatcca
     9661 gatagtggat ggggatttat gacttgggat gaaaatggag aataataaaa attatagttt
     9721 atacagttta ataagatata tgtacaagca gttaatctaa atgattgact gcttttcttt
     9781 tatagatttt tatataatag acaaatacca atacatgata gttgcctgtt ataattagag
     9841 gaataaatac gaataataac tttataaaag gattaaatgg aaaatcgaag agttatattc
     9901 agtattttaa aaagaatatg aaatgagaaa tgaaaataag gtgtttatat taaaagaggt
     9961 tttacacaaa atatttaaaa acgtgataaa taaaaactgt atttagatag attaactatt
    10021 agttttatat aatatggatt ttatttacaa agtttacata ttttacaaag gagattaaag
    10081 ataaatttaa atatatattt tatatacttg tttatttgtt tctctgtggt tgattttttc
    10141 gtcaaaagtc aacttcaaat cgcttgttag ctttacatgt ttaaggatat aattatacat
    10201 aaggagggat tgacatgaat gaattaaaca tcgcaaaaac gctgatttta aagcgtaaag
    10261 aaaaaggaat tacacaagat gaacttgcaa attatatagg cgtttctaaa gcttctgtat
    10321 ctaaatggga aactggacaa agctatcctg acataacctt tttaccgcaa ctcgcgacat
    10381 atttcaacat taccgttgat gagttaatat gttatgaacc tcaaatgatg aaggaagata
    10441 ttaataaact gtataataaa ctgtgcaaag actttacggt taaaccattt gatgaagtca
    10501 tggatgaaat aagagaaatc ataaaaaaat attattcatg ttttcctttg atttttcgga
    10561 tgggtctttt aatagttaat cactatgata ttgtagatga aaaaaagcgt gaactattaa
    10621 ttagtgaagc attagaaata tttattcgta tacaggaaac ttgtaatgac atagatattt
    10681 gccgtcaagc aaaaagtatg gaggcaactt gttatatatt gttaaatcaa cccatacaag
    10741 tgattgattt attgcaaaac agcaattttc ccatgataaa tgaatctata cttcttgcac
    10801 aagggcaaat gatgaatggt caaatggatg aagcaagaga aacatttcaa cttggtgcat
    10861 atcagaacct tatatcatta gtccaaaatc tagtgggtat tttgcaaaat gcagataaat
    10921 tacaaatgaa ggaaatagaa agacgtattc tagcaatttc agatatattt gagcttgata
    10981 cattatctcc tgctattatg ctttcttcat atttaacaca ggcacaaatt aatttaatac
    11041 atggtgataa tgaaggagct attaaatcat tacgaaaata tgtagattta gcaacacgtg
    11101 atatataccc aattataata catggggatg acttttttaa taaacttgac agatggattt
    11161 ctgaaattgg aacaggtatt acacgtgatg atacaatcgt aaaagcaggt attgttgctg
    11221 ctattaaaaa taatccaatg ttttctgtat taagtgaaaa caaagaatac aaatttctta
    11281 tagaaaaatt atctttattg gaggaataag attatggaaa atttgatgga atacttacca
    11341 cttttggttc ctgttattat tttagattta atacttataa ttactgcact tgttcatgtg
    11401 ttgagacatc caaattataa aattggaaat aaggcaatat ggattattgt agtactgttt
    11461 attagtttaa taggaccaat tttgtatttt acaataggaa gaggtgaaga atgatgaata
    11521 ttgtctcaat aaatggatta tcaaaaggtt ttggtaatcg aaagataata gataatttaa
    11581 attttactgt accagaaggg tcagtctttg ggtttgtagg taagaatggt gcaggtaaga
    11641 ctacaacaat gaaaatggta cttgggttac ttaaaccaga tagtggaact atagatgttt
    11701 gtggtgagaa agtgacctat gggaaaacaa gttctaatcg tcatgtaggt tatctgcctg
    11761 atgttccaga gttttacaat tatatgagac cacttgaata tctttctctt tgtggtgaaa
    11821 ttacgggact ttcaaaaaaa gaaatacaga taaggagtga agagttgtta tcacttgtgg
    11881 gtcttagaaa tgaaaagaga cgaataggtg ggttttctcg tggaatgaag caacgacttg
    11941 gtatagctca agctttgctt tcacgcccca aattacttat ttgtgatgaa ccaacaagtg
    12001 cacttgaccc tgttggtcga aaagaaatat tagatattat gcttaaaatc aaagactcaa
    12061 caactgtaat attttcaaca catatactat cagatgttga aagaatctgt gaccatgttg
    12121 ctatattaaa caaaggaagc attgccctta gtggtacact ttcagaaata aaaagtatgc
    12181 acggaaagga tagactttta ctggagtttg caagtaatga tgaaattcaa aagtttaagt
    12241 ctagtgatgg tattaaatcc ttactaaaag attcagaaga aactaatatg gaaattgttt
    12301 tgcatggtaa agatataaaa gcaatacaaa aaacagttat atctacgctc gcagaaatga
    12361 atctttgtcc agtaaagatg gaactaatag aattatcact tgaaaattta tttttggagg
    12421 tagtaaagtg agagaataca tagcatttac aaaaaaagaa tttaaagaga atttaagaaa
    12481 ttataaatta tttagtttaa ttattttatt tctaatattt ggtataagta gtcctatttc
    12541 tgcaaaattt atgccagact tgattgctca ttttgcaccg actttaaaag ttactgctgc
    12601 accaactgct ttagattctt ggacacaatt ttttagcaat atctctggtc ttggtatgag
    12661 tttaactcta attatttttt gtaatatttt atcaaatgaa tattctaaag ggacacttgt
    12721 tattatgctg actaaaggtt tatcacgttc atctatagta ttgtctaaat tctctgttac
    12781 ggtgattatt atgacaattg gtttttggtt gagtttttta tttgcttatg gctatacaat
    12841 gtatttttgg ccaactgcaa atcttaatca tattatattt tcagcattta atttatggct
    12901 tataggaatt atgtatatta gtgtattaat tcttgggtgt gtactattta gaccagcatt
    12961 tgccagtgtt ttacttgtat tggtagcaac tctcttattg agtttaattt ctattcctaa
    13021 acagattgca ccttatactc ctaatttctt gatgtcaaaa aatatagact tgatttcagg
    13081 gaaagtggca atgccagaat ttattattcc aattattata acagttctta tttctatagt
    13141 atgtttacta gcagctgtta ttttatttaa taaaaaacca gtttaataat acctttatca
    13201 caaggtaaag taatcttatg ttgttaaaaa ctacacctaa aaatcaaaaa gacttggact
    13261 taagtttcaa gtctttttga tttttaggtg tagtttaata tatagaatta agtgtagaaa
    13321 ataatacaaa taatatta
//
LOCUS       DACULQ010000110        13264 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.110, whole genome shotgun sequence.
ACCESSION   DACULQ010000110 DACULQ010000000
VERSION     DACULQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.110"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            149..2008
                     /locus_tag="JA154_12575"
     CDS             149..2008
                     /locus_tag="JA154_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HAT7996683.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2300..2899)
                     /locus_tag="JA154_12580"
     CDS             complement(2300..2899)
                     /locus_tag="JA154_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tellurium resistance"
                     /protein_id="HAT7996684.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3002..3799)
                     /locus_tag="JA154_12585"
     CDS             complement(3002..3799)
                     /locus_tag="JA154_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAT7996685.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3912..4017)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4109..5095)
                     /locus_tag="JA154_12590"
     CDS             complement(4109..5095)
                     /locus_tag="JA154_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAT7996686.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5579..7309
                     /locus_tag="JA154_12595"
     CDS             5579..7309
                     /locus_tag="JA154_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HAT7996687.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7419..7499
                     /locus_tag="JA154_12600"
     tRNA            7419..7499
                     /locus_tag="JA154_12600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7453..7455,aa:Leu,seq:tag)
     gene            7834..8349
                     /locus_tag="JA154_12605"
     CDS             7834..8349
                     /locus_tag="JA154_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HAT7996688.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8841..8945
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9155..11629
                     /locus_tag="JA154_12610"
     CDS             9155..11629
                     /locus_tag="JA154_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HAT7996689.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSILRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYIATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11629..13086
                     /locus_tag="JA154_12615"
     CDS             11629..13086
                     /locus_tag="JA154_12615"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088159.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HAT7996690.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         4971 a         1574 c         2193 g         4526 t
ORIGIN      
        1 gaggactcta aagatgaact atgtaaaaca aggtttatat attctaattg tatagaaaat
       61 ttattgtaaa cgcatgtata ttattagaaa aaatagtttg aaggtattca ataaacataa
      121 aaaacaatat aggggataaa ggtgttttat gaagagattt ttgaggagaa ttatcttagt
      181 tttatttatt ttgttgattt ttataagttt tatttctatt aaattaattc ataatgttgg
      241 agattatgga aaattgatta attatgttgg gattgttcgt ggagctagcc aacgtctaac
      301 taaactagag atgaatcatc aaccaaatga tgaattaata gaatatattg atgaaatcct
      361 acaggagttg attacagggc atggagatta tggtttagta atcactgatt gtaatgaata
      421 caatgaggac ttacttttat tagaaaaaaa gtgggaagat ttaaatattg aaattaaaaa
      481 agtaagaatg aaagaacaga ataaccagtt attaagtata agtgaagagt ttttttctct
      541 tgcaaatgat acagtgttta aaatagaaaa cttttctaaa gaaaaaagta attacttaat
      601 gactttaata ataattatat ctattatagg tatattagca tgtatcatat tgattttaca
      661 atatagtaaa aaaatggtta gactggaaaa attaaatgta gatttaaaaa atattgcata
      721 taaagatgaa ctaacaggag taaatactat agaaaaattt aaattggatg caaatcaaaa
      781 tatttgtata cataaggata agaaatttgc tgtattctat attgattttg aaaactttaa
      841 atacattaat gatatatttg gatatgatta tggtgacatg atactgaaaa gatatgctaa
      901 tcttatgatg aatgacatag ggaaatatga aatatttgca agagaaattg cagatagatt
      961 tgttgcgtta agatgctatg tagataagga agatttagtg gtaagacaac gagatgtaga
     1021 caatgaacta attaatataa caaatgaaat taaaaataaa catagtatta ctgttgttag
     1081 tggaatttgt tgtattgaag atgtaaatga aaaattaagt atagatggtt taataaatag
     1141 agcaaatttt gcacaaaaaa ctgttaagaa taaaccaggt actaattatg ctttttataa
     1201 tgacagtatt cgtaaaaaaa tgattgaaga aaatactatc aagagtagaa tacatgaagc
     1261 tattgaaaaa agagaattta tagtttattt acagcctaaa gttaatttac atagtcaaaa
     1321 aataaattgt gcagaagctt tagttcgttg gcttactccg gataaaggta ttatatcgcc
     1381 agcaatattt attccagtac ttgagaagaa tttttttatt gccttagtag ataagtatgt
     1441 ttttgaggag gtttgtaaat ggattagaaa gaggttagat gaaaataaac cttttgttca
     1501 gatatcagtt aatgtttcaa gaatacaatt ttataacact aaatttgtag aaacttattc
     1561 caatatacaa aataaataca ggatacctaa aaatactata gaaattgaat ttacagagag
     1621 tgttgctttt gagaatcaaa aacatctttt ggaaattata catgatttgc atgaaaatgg
     1681 atttacttgt tctttagatg attttggaaa aggttattcg tctttaagtg ttttaaaaga
     1741 tttaccattt gatgttttaa agcttgattc tatgtttttc aaggctagtt tagataaaga
     1801 taaagagaaa atagtaataa aaaatattgt acatatgtta aaagaactta atatcacaac
     1861 tgttgctgag ggcattgaat gcaaggaaca agttgaattt cttagggata ttgggtgtga
     1921 tttagttcag ggatttgtat tttataaacc aatgccaata ttagaatttg aaaaaatatt
     1981 ggataaagag tttgtttata atagctaaaa tgtactttat aaaatatgaa ctaattaagt
     2041 aaaattattt tgtactgaat aaatttttac tttatttaca gaataatgat tatttacaga
     2101 aaagcgcgaa aagtgtattc ctttaagtgt gggatggata gcacaaatag aggagaaaaa
     2161 atattggcca tataagaatg cataataata taaatatttg aaaggtcagc attatataaa
     2221 aattaaaaag aaaataaata atttttctat aaaaaagaga ctatgagaaa atttactcat
     2281 agtctcctaa ataatgtatt tattttcttc ctgcaaccca ttttaatccc caatgaaatt
     2341 cagtatccat ttctctatga cctttgaagt atttaactac tttttcaaca ctgaatgtct
     2401 catcatttac attttctatc aatgctattg aacacatatt atagccattt ttatgttcat
     2461 caagtttgac aactaagtct gaatcttgtt tttgtttgat tgttacaatt ccatcagctt
     2521 cagaccagtt agctacacct tcgtatataa aagcataaat aagtattctt tttatatctt
     2581 ttattttatt accatttatt cttaaatttt ctccttgtgt attacttcct gttctgtcat
     2641 ctccatctaa ttgagcaaaa ggtggattgt ctaaactacc aaaggcattt cctaatgctt
     2701 gaacagctcc ttttacacca tttttcatct caaataggca acccaaatct aaatctatat
     2761 tactgcttct taaagagcca aaaaaaccct ttttttgtac tttttgattc caatttaaat
     2821 taactaagat ttcacctaaa tcgctatttt ccttttttgt aaggtttatt ttttgtccct
     2881 tttttaattc tatagccata gtaatcttcc tccacaaaca tagatttatt taaatggtag
     2941 cacttaataa gtcaatattc aacaagatta gaataataat attaaaatta tactaaatat
     3001 actaaaaagt aggaatcaca atacctttgt atttttcttc aattaagctt cttgctttat
     3061 ctgatgtaag aacatcttgt agaactttaa tcttttcact gtctttatct tcagttctaa
     3121 atgctaaaag atttgcatac tcgtcattta taggagctgt ataaattccg tttttcttgg
     3181 catcaagacc agcaggtaca gcataagtag tatctataaa tgcagcagtc acatctggaa
     3241 gagaagatgg aatttgagca atatctaact ctttgaattg tattcccttt ggattttcta
     3301 ttatgtcctt aggactaact aatccatccc ctttaggtaa cgttaaaagt ccaatacttt
     3361 caaggtatct aaggttctta gaacaagaag atgggttatt gcttattgca attgtatctc
     3421 cttttttaaa ttcatcaaca gattttatct tataagagta aagtattgct gggcatacat
     3481 atagtttttt accagccatt attttgtaac ctttggcctt aacagcttct tttagataag
     3541 gttcatgttg aaatagattt gcatcaattt caccattatt taaagcttcg tttggaagta
     3601 tgtaatcatc aaagttttta acttctaaag tatatccttt ttctttaata agtggtttta
     3661 attcttctaa taactctcct cctggaacta aagtagcacc aacaacaatt tttttatcat
     3721 ctttagcttt agaacatcca accacagcta aagttaatac tagacataat aacaaacata
     3781 ataatttttt gaatttcata taaattccct cctgaatata attgattagt taaaaataaa
     3841 ataccattct ttatcacact gcaaaataaa aataagcctg agtttaaact caggctttaa
     3901 aagctaattt agactcatct ttcagtataa tactgcagga tttagcacca atgtatataa
     3961 aaatatactg gttgctgggt ttcatagggc cagtcccttc accactctgg ataagaagta
     4021 tcaattttta attatgttat aaatattatc atgatttttt acgtctgtaa acataatttt
     4081 ataaatattt taaaatcttt aatgtatttt atttaacacc taaaattcca gaaactagaa
     4141 tatcagtatc agcacctaag aaatagtcat ttatttcaat agaatccaga gtatttctca
     4201 aagcattttc ttcatgcctt ataccaataa gtttattttc tacaaatgca acatcacatt
     4261 ttccaaaaaa atctccaaaa aattttatac tagttattat gcctttatca acatttaaat
     4321 taaactcaac atttccacca gaatatttta attcatttga aagagtatat tttggtgaat
     4381 taccaaaatt ccaatcccaa gtttcgtatt ttgatttagt aagcttttct atttgtttta
     4441 tatcatgctc actcaaacaa taattagtat tatcagtatt tgttttagaa agataatcca
     4501 ttatagcatc tttaaattct aatatgtcca tttttgaatg taaatgttca cttatattgg
     4561 ttacacgaga ttttacagat tttattcctt ttccttcaaa ttttataggt ttaaccttta
     4621 atgcttttga gaggtcattt atttcagatg aaaataaaag agtaccatga tgcattactt
     4681 tgtttttgta gttgtattga gcatttccag aaaatttctt gccatctata agaaggtcat
     4741 ttcttccaga aaattcggca ttgacatcta gtgttttaag taaatctatt atgggctgag
     4801 tgaatcttct gaaatcacta aaattattat tgttacaagc tataaatgta aaatttatat
     4861 ttcccaaatc atgaaataca gctccaccac cagactgtct cctaacgaca ggtatagaat
     4921 attcttttat atattctaaa tttatttcag aaagtgtgtt ttggtttttt ccaacaatta
     4981 tagaaggttc attcctccat aaaataaata agtcttcatc aaaatttttt agaagatatt
     5041 cttccattgc taaattaaaa taagggtttg ttttttcatt ataaattaat aacattaaag
     5101 tagttcctcc atagaattga taaaattttt attatgaata caattaattt tataattcga
     5161 caataatata aaaactcctt taaattagaa attttaaaaa ctaccttgca aagtttaaat
     5221 aaataatata tactcaaaat atgtttaata aaagtgaata gaagggtaga ggcgcaataa
     5281 tctatcagta ctaataagga gttaagcaca gagatttatt aggaaaggag atattgccga
     5341 agttttataa tcgatgcttt aagattatat tgctgggggt acatagaaaa tatgtacaac
     5401 tgtcacaggt ttatttgtgg tgagctatca tttaaggaat tatattatat ttatttttac
     5461 tattgttgat atattgtaat gtttaagcct tgagtgttta ccactcaagg cttttttagt
     5521 tttattgtaa cataataccc tgtacagttc acaaaattaa aaggagaggg agatttatat
     5581 gaggaacaaa aaaattaata ttatttttct gacgacaatc atgttcataa tgtcaacagt
     5641 aatggttttt gcagaagaag atatagacac tattgcacta gccaatgcag aaaaatttgg
     5701 gattttaact ttaataccac cattagttgc tataatactt gcatttataa ctaaaaacgt
     5761 aataatttct ttacttatag gtattctttc tggtagtttt ataattaaag ctagtggtat
     5821 taatgttttt gcgacattta tacaggcatt tttagattta gtagacagag ctttagtatc
     5881 tttagcagac ccttggaatg cagggataat actacaggtt ttggcaattg gaggagtaat
     5941 aaatcttgtt gctaaaatgg gaggagctaa agctatagct gaagcacttg ctaaaagagc
     6001 aaagtcagca aaagggacac agcttataac atggtttttg gggcttttag tattctttga
     6061 tgattatgcc aattcactaa tagtaggtcc tatgatgaga cctgttgcag ataaaatgaa
     6121 aatatcgaga gaaaagctag catttataat agatgccaca gcagcaccag ttgctggttt
     6181 agctataata tctacatgga taggtcttga ggttggttta atacatgatg cttttgaaag
     6241 tataagtata gatgtagatg catttggtat atttttaaat acaataccat ttagatttta
     6301 caatatatta atattagctt tcattgtaat atcagcatta ttattaaaag aatttggtcc
     6361 aatgagaaaa gctgagataa aatctagaag tagaaaaatt agtatagacc ttgatgaagg
     6421 cgtagaagag ttggatgatt tagctccaaa aaatggagta aaattgagtg tatggaatgc
     6481 tataatccca ataggaacat tgataatagt tgcattagca tcattttatt atagtggata
     6541 tacatctata atgggaggag acgataaggc tctgatacaa ctatttacaa attctccata
     6601 ttcttttgaa gcaataaaag aagcatttag tgcatctgat gcatctagag cattattcca
     6661 gtctgcactt gttgcaagtt tagttgctat tataatggct gtagtaaaaa agatttttac
     6721 aatatcagaa gcaattgatg tttggataga tggaatgaaa agtttagtaa tcactggtgt
     6781 aatattaata cttgcatggt cattaagttc agtaataaaa gagttaggta cagctaaatt
     6841 tttaatacac ctattatcag gttcactacc accattctta ttaccaagtt taatatttgg
     6901 tttaggagca ataatatctt ttgctactgg aacagcttat ggaacaatgg gtatacttat
     6961 gccacttgca attccattag catactcatt aaatcctgat atgtcttatg taatagtaag
     7021 tactagtgca gttttaacag gtgcaatatt tggagaccat tgttctccaa tttcagatac
     7081 aacaatactt tcttcaatgg gagctggctg taatcatata gaccatgtta acactcaaat
     7141 gccatatgct atatttacag cagtaatcac tattgtattt gggtatatac cagctggact
     7201 tggattgcca atatatatag tattaccagt ggcaatagct gctatatttg taggtattca
     7261 aataattggt aagaaagttg atgaagcgga aatagaactt gtagaataaa gatataaata
     7321 agtttcacct tttattatca ctattttgtt agtttttaga taattttaaa aagttattga
     7381 aaataatggc gaaatatagt aaaataaaag agtgataagc ggatgtggcg gaattggcag
     7441 acgcactaga cttaggatct agcgccattg gcgtgggggt tcgactccct tcatccgcac
     7501 tttacagtac ttaattgtac tgaaaaaaac tggggttgct ggctttcgga gtgggctatc
     7561 ccgtttttat ttttttgtat atataataga ttggcacttt agtataaggt gcttttttta
     7621 taggcaaaaa cagtacattt atttctgttt tattatcatt catctattag aatatacttt
     7681 agttaagaat gtgttaataa gcagtgttca cattcttgat aaaatgtgga ataatatgta
     7741 tttttactaa gacggaaatg aaataattaa accattaaat aatattataa gatgatgaat
     7801 aagtagataa atcaaatttt gtaggaggat attatggaaa aatatatatg gttgtttcca
     7861 ttacttttta tttttcatga tatggaagag ataataggtt ttggaatatg gcttaaaaaa
     7921 aataaatcaa tgcttgataa gaagtatcca tttatcagta aagtttatga aaattatagt
     7981 actgaaggca tggcatttgc tgtgtttgaa gaatttattt tatgcattat tttctgtata
     8041 cttacagtta taacagagaa tcagtatgta tatctactat ggttaggtag ttttatagca
     8101 tataccttac atttggtaat tcatataggg cagtcaatca taataagaaa atatatacct
     8161 tcacttatta ccagtatcat atgcttacca ataagcattt ggtgtataag taagagtatt
     8221 tatatagttg attgtgaaat gagtacaaca attctgtaca gtataattgg cattataatt
     8281 gtagcattga atttaaagtt tgcacagtcg ctgattggaa aatttacaaa gtggatgtca
     8341 aacatataaa ttttagttta ttggacagtt caaggaatca gaaattggaa ttttatagta
     8401 ttaatatttt atggtattaa tataatgtat aaaattttat agcaatgtat ttcataagta
     8461 taaagtgact agaattttaa gaaatataca aattctataa aaactaaata atttcaagta
     8521 tattttaaaa atcttatttt tcacaagttt aaaattgtaa acgatttcat caagtctatg
     8581 aatatacttt taacatgtta tagtaaatct caatataaat ttacatttgg ttctttgtgt
     8641 gttattttaa taataaatta agtgaaaatg ttattttaca ttaatatata atatctttgc
     8701 ataagagtga ataatataaa agagaaataa ttgataataa aaacttaaca aaaaaattaa
     8761 tacataaaaa aaaataaata taatgtgttg acatttaatt gaaatattca tacaataaaa
     8821 ttgaaaataa atatttccaa atgagattag cgagagagtc ctttatacga aaaggcgccg
     8881 aagaagaaat ttaagaaatg ccaattttta gagaagcttt caggcaaaaa tatcgctaat
     8941 tgatgggact ctggagagac ttaagttaag atactataag tcaccgaaga agcaaaataa
     9001 tattaaatta ttaaatcttt caggtataca aacagggata tataggctag gattattttc
     9061 catgctgata tatctttttt ttatgaaaaa gtacaggaaa taatatcaaa gttacttaaa
     9121 tacagtaggt ctaattttct agggagggtt atatatgaat gaattgaaaa gagtatcttt
     9181 gtacaatata cacaaagagc ttggagcaaa acttgttgaa tttgctggtt gggaaatgcc
     9241 tttagaatat gagggtataa ataaagagca tgagaaggtc agaaaaagtg caggtatatt
     9301 tgatgtatct catatgggag aggtacagat aaagggagca gagtcagaaa aatttataca
     9361 aaacctagtt acaaatgata taagcgcatt aaagataaat gatattatat atacacctat
     9421 gtgttatgaa aatggtggag tagttgatga tttattaata tataaatttg gtgaagaaga
     9481 ttatttatta gtaataaatg caggtaatat agataaagat gtagcatgga taataaaaca
     9541 aagtgaagga tacaatgtag atataaaaaa tatatctagt gaagtgagtc aattagcaat
     9601 tcaaggacct aaagcagaag aaattcttca aaaaatcaca gatatagact taaactcaat
     9661 aaaattttat aagtcaattc catcaacaaa agtatgtggt tgtccttgtt tagtttcaag
     9721 aacaggatat actggggaag atgggtttga gatatactgt aaaaataaat atgtagaaat
     9781 aatttggaat gaagttctaa aggttggagg agaagatata tgtccagctg gtctaggttg
     9841 tagggatact ttgagatttg aagcagctct acctctatat ggacatgaaa taaatgaaca
     9901 catatctcca attgaaggtg gtttatctat ctttgtgaaa acaaataaag aatcatttat
     9961 tggcaaatca attttatcaa aagaaaaaga aagtggagct aaaagaaagt tagtaggatt
    10021 tgaaatgcaa ggtaaaggaa tgcctagaaa tggatatgat ataaggattg gtgataaaac
    10081 agttggattt gtaacaacag gatgtgcatc tccaacgaca ggaaaaattt taggtatggg
    10141 aattatagat tctgaatatg caaaagtagg aaatgaaata ggtatagcaa tcagaaaaaa
    10201 agttgttcct gctgtgatag ttaagaaacc tttttataaa aaacaatata aaaaagataa
    10261 tattatatta aacaaagaaa ataaattttc atatatacct gctacaagtg aagataaatc
    10321 aaaaatgtta aaagtagttg gattaaattc ggttgatgaa ttattttcag atataccaga
    10381 agaagtaaaa ttaaaaagag atttaaattt ggaaattgga aaatctgaat tagaggtaag
    10441 caaaatagta aaaagattgt cagaagaaaa tttaagttta gaagacttga cttgttttct
    10501 tggtgctggt gcatatgacc attatatacc atcaattata aaacatataa cttcaagatc
    10561 tgaattttat acagcatata ctccatatca agctgaaata agtcagggaa ctttacaagt
    10621 agtttttgaa tttcaatcta tgattgcaga aattacaggt atggaaattg caaatgcatc
    10681 aatgtatgat ggagcaactg ctgcaataga agcatgtata atggcaatga atcagactag
    10741 aaaaagtaaa atagtagtgt caaaaactat tcatcctgaa actttgtcaa tattaagaac
    10801 ttacttacaa tataaagatt gtgagatagt agaaattgac ttttgtaatg aatatggcac
    10861 tacagatata gaaaaattaa aagcttctgt agacaaagat acagcatgtg ttcttataca
    10921 aactcctaat ttctttggaa ttattgaaga aatggaagaa atagaaaaga taactcatga
    10981 aaataaggct atgttaatta tgagtgtaga cccaatatca ttaggagttt taaaaacacc
    11041 aggtgagata ggtgcagata ttgttgttgg agaagctcaa tcacttggaa accctcttaa
    11101 ttttggaggt ccttatgtag gatttttagc tagcaaatct aaatatacta gaaaaatgcc
    11161 aggaagaata gttgggcaaa gtttagatgt agaaggtaaa attgcatatg tattaacttt
    11221 acaaactaga gaacagcatg taagaagaga aaaagcaact tctaatatat gttcaaatca
    11281 agctttaaat gctcttgtag cttcaatata tatagctact atgggtaaag aaggatttaa
    11341 agaggttggt atgcagtcta tgaaaaaagc acattatact tacaataagc ttgtacaaac
    11401 tggaaaatat aagccaatat ttaaaggtaa gtttttcaaa gaatttgctg tacaaggaaa
    11461 tcttaatata gaaactataa atgataaact cttagaagaa aatatattag gtggttataa
    11521 tttagaatac aattatccag aattaaaaaa ttcaactttg ctttgtgtga ctgagaaaag
    11581 aagcaaagag gaaatagata agttagttgg aataatggag ggattataat gaaagaatac
    11641 aatagtttgt tgatagatat atctaaaaaa ggtaggaaag catactcact tccaaagtta
    11701 gatatagatg atataaaaat agaaaatatg attgaccaag atatggcaag acaaagtgag
    11761 ttgaatttgc cagaagttgg tgaattagaa ttagttagac attatacatt attatcaaat
    11821 aaaaactttg gagttgatac aggtttttat cctttgggtt cttgtactat gaaatataat
    11881 cctaaaataa atgaagatat ggcagcactt ccaaacttta ctggaatgca tccataccaa
    11941 tcttcggata cagctcaagg ttctctttct ttgatgtatg atttatcaag aagacttgca
    12001 gaaattacag gtatggatga agttacacta caaccttcag caggttctca tggtgagttt
    12061 acagggctta tgattataaa agcatatcat gaaaatagag gtgaccataa gagaaccaaa
    12121 gtgataattc cagattctgc acatggaaca aatccagcaa gtgcagctat ggcaaacttt
    12181 gatgtgattc aaatagcatc tgataaaaat ggagctgtag acataaatgc attaaaagag
    12241 gttttgaatg atgaagtagc agctcttatg cttactaatc caagtactct tggtttattt
    12301 gaaaaaaata taaaagaaat agctactctt gtacatgaag caggaggact tttgtactat
    12361 gatggtgcaa acatgaacgc tattatgggg ataacaagac ctggagatat ggggtttgat
    12421 gtggttcatc ttaatcttca taagactttt tcaactcctc atggaggtgg aggtccagga
    12481 gctggtcctg ttggagttaa aaaagaatta gtaccttttt taccaatacc agtaattgaa
    12541 agaaaagaag ataagtatgt actaaattat gatagagaaa aatctattgg gaaaattaaa
    12601 aatttctatg gtaattttgg agttcttgta agagcttaca catatatact aactatggga
    12661 agggatggac ttaaagaggc tagtgaaatg gcagttttaa atgctaatta tataaaggaa
    12721 agtataaaag atgattatat tttgccaata gataccttgt gtaagcatga gtttgtatta
    12781 ggtggattgc caagaggaga ggccaatata aaaactatcg atattgcaaa aagactacta
    12841 gattatggat accatcctcc aacaatgtac tttcctctta ttataaatga ggcacttatg
    12901 atagaaccta cagaaagtga gagtatagag acattagata gttttataga agctatgaaa
    12961 agtgttgcta aagaggcaaa ggaagaacca gaactattga aaactgctcc acataatact
    13021 ttggtcaaaa gagtagatga tgcaagggca gtaaagaaac ctatactaac atggtcacaa
    13081 agataaatag ttaaaaagta gagatttaaa gtataaagag aattaaattt gtaagaaaaa
    13141 gctatctaac ttaatctagt ttagatagct tttttgtata ttagtatgta tggtattttc
    13201 tatataaagt tttaaattat attagtgctt atatagaata ttgatatgat gattatgtat
    13261 agat
//
LOCUS       DACULQ010000111        13258 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.111, whole genome shotgun sequence.
ACCESSION   DACULQ010000111 DACULQ010000000
VERSION     DACULQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.111"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            337..1386
                     /locus_tag="JA154_12620"
     CDS             337..1386
                     /locus_tag="JA154_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase"
                     /protein_id="HAT7996691.1"
                     /translation="MEVILLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQ
                     YTDLYSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKK
                     VMTVGFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTED
                     VKLLRSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKD
                     MKSFLIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKE
                     NVEKENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDI
                     SSSTLGKRYKDLKIS"
     gene            1467..1832
                     /locus_tag="JA154_12625"
     CDS             1467..1832
                     /locus_tag="JA154_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996692.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            1837..2154
                     /locus_tag="JA154_12630"
     CDS             1837..2154
                     /locus_tag="JA154_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996693.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            2202..3806
                     /locus_tag="JA154_12635"
     CDS             2202..3806
                     /locus_tag="JA154_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996694.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            3930..5558
                     /locus_tag="JA154_12640"
     CDS             3930..5558
                     /locus_tag="JA154_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent phosphate transporter"
                     /protein_id="HAT7996695.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTSLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAALDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNIYFSEEGQSELD
                     VMYNRVVSAYTYALESMRSDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            5823..7172
                     /locus_tag="JA154_12645"
     CDS             5823..7172
                     /locus_tag="JA154_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAT7996696.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            7328..8407
                     /locus_tag="JA154_12650"
     CDS             7328..8407
                     /locus_tag="JA154_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HAT7996697.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            8752..9660
                     /locus_tag="JA154_12655"
     CDS             8752..9660
                     /locus_tag="JA154_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAT7996698.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            9665..12034
                     /locus_tag="JA154_12660"
     CDS             9665..12034
                     /locus_tag="JA154_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HAT7996699.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKQDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            12359..13162
                     /locus_tag="JA154_12665"
     CDS             12359..13162
                     /locus_tag="JA154_12665"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089797.1"
                     /note="catalyzes the formation of L-proline from
                     pyrroline-5-carboxylate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAT7996700.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         5354 a         1406 c         2481 g         4017 t
ORIGIN      
        1 aaagtcaata attttgatgt tcaaaatatt ccaaatgaat ataaacaaga aaaagacaat
       61 attgttgaat gtaaaatgac agttagagtc atataacaaa aaagaataga taaagtaaaa
      121 aactattatc aattaataaa attttataga ggaattagtt ttacgataat tcatttctac
      181 aagagggatt gttgatgatt ttatcgaaat cctaattaaa gttaatatat tgtaccaaaa
      241 tataagtgaa tttaatagaa tatttactta tactatagat tgattaataa aaaaatggta
      301 taataacaaa ttgtacttta atttaataat aagggagtgg aagtcatatt gttagggaga
      361 aatgagctat gcccttgtgg aagtggcaaa aaatacaaga aatgttgttt aaataaagat
      421 attgtttcag aaagagcatg cagaaaaact gaactttcac aaaaacaata tacagattta
      481 tattcaaagt tatatgatta ttcaagacag gataagttta aagaagaatg tgaaaaagct
      541 aaagaaatgt tttatataat gcaaaatgat agtgtaaatg aaaaatttga aagatttttt
      601 aatacttatt taatacaaga ccacataatg gaaaataaaa aggttatgac agttggtttt
      661 cttgaagaaa atggtgcaaa cctaagtcaa tcagaagtat caatattaaa aagtatgttt
      721 gaatcatacg taagtattta tgaggtaaaa gaagtttctg ctggaaaaat agtattaaag
      781 gactgtttaa gtggaaatga actttgtaca gaagatgtaa aacttctgag aagttttaaa
      841 atcggaagtt gtatgatagc tagaatagtt gatattgaag gaactaacat attgatagat
      901 ataactataa gtatatcaaa tgaggttaaa gatataattt taaaggatat tatgaatcta
      961 ttcaatcaat atcaagatgt atacaaagat atgaagtcgt tcttaattta tcatacacat
     1021 atactataca agtatataca acagctttta gacccaagta tagctgaata tttaaagaga
     1081 gaaagagata aaaacgatac aaaagttaat tcagaaaatg aattagctga agaagaatgt
     1141 aaagttttag atactcttaa agaaaatgta gaaaaagaaa atatagatgc atgtatagag
     1201 ttttggaatg aatataagaa aaatcatgaa aatataaaag gagctgaaaa tggttgggca
     1261 gctgctgttg aatactatgt taaaaaagaa gcaggacaac ctataactca agttcaaata
     1321 tcaaagaaat acgatataag ctctagtaca ctaggaaaga gatacaagga tttaaaaata
     1381 tcttaattaa ttataaaaag ctttaagtca tagataaaga atctatagta taatatatgt
     1441 ataatgttta gaaagaggta ttaataatga gtcttagttt aggaaatgca attgctgtat
     1501 caacgaactt tataaaatct aacccagctg tacctgtgta caatggagaa gaaatatatg
     1561 cagttattta tcatatagaa ataggatata ataatggtgt atcacctgat accaaacaat
     1621 atataataat agactataaa gagtttgatt tatctatgga taaaaaaaat gaactaataa
     1681 atattgctaa agagtactgt aaatctaaag gtattgtaga aaacgaaaac cttgaaataa
     1741 accttttaga caatgaagaa gcagagaact tcttagaaaa ggtgtttaat aatatgaagg
     1801 ttataagagg tcttataact aaaaaaggat aaataaatgg actatatagg aatagaaaat
     1861 ataacacctt atgaaaatac atatgaattt agtgtatatg aatatgatga tgaaatcacc
     1921 ttaggtagtg aaaagttata tgtatgtgaa ttaagggttg tattgattaa agttaattct
     1981 ctgtatgttg aaagattgca taaatcagtt gaagcaatgg tcttagtaaa aaacttgaaa
     2041 aaagatttag ataaaacact tgttgtaaac aaaataaaga attttgtgct agatgagatt
     2101 tgggtagaaa atctagtaaa agagaatata gaagttatat ttgtagaaag ctaggaataa
     2161 ccctagcttt tttatattac aatctaagag aggaaaacaa tatgttaaga gtatctaatg
     2221 taaaacttgg aattgataaa gatatatctt tattaaaaga tgttatactt aagaagctaa
     2281 aaataaaaga aaaaaactta gtcaaatata gtatatataa agaatctata gatgctagaa
     2341 aaaaaggaaa aatggaattt gtttattctg tagatgtaga agttaaagat gaaaataaaa
     2401 tacttaactc aaaaataaaa gatgtaacaa aaataaagga aatcagatac gtaaatgtac
     2461 ctgtgggtga taaaaaatta aagaataatc ctttggtaat tggtagtgga ccagctggtt
     2521 tgtttgcagg tctgctttta gctcaaatgg gttatgaacc aatcatactt gaaagaggtc
     2581 ttgatgtaga cagtagaacc aaagatataa gtgatttttg gacaactaga aaattaaaaa
     2641 ataattcaaa cgttcaattt ggagaaggtg gagcaggaac tttttcggat ggtaaactta
     2701 caactagaat aaaagatata cgatgtagaa aagtattaga agagttagtt cagtttgggg
     2761 caccagatga aatattatac tcacataagc cacatgttgg aacagatata ttaaaaaatg
     2821 tagttaaaaa tataagaaat gaaataataa gtttaggtgg tcaagttaga tttaattcaa
     2881 aagtaacaga tatagttgta tcaaatgatt ctatacaatc tgtaataata aatgatactg
     2941 aaaaaataga tacagataca attatattag ctgttggaca tagtgcaaga gatacttatg
     3001 aaatgttgta ttcaagaggt ataaaaatag ttcaaaaacc ttttgcaata ggagctagag
     3061 ttgaacatcc acaagattta ataaataaag cacagtacaa ggatttttat aatcacccta
     3121 ggttaggagc tgcagattat agacttatag agcatgtttc aaatggaaga acagcatata
     3181 ctttttgtat gtgtccagga ggtagtgtca tagcatctgc atcaaatgat tttgaaatag
     3241 ttacaaatgg tatgagtgaa catgcaagag ataaaataaa tgcaaatagt gcatttttag
     3301 taaatgtaac gccagaagat tttaagagtg ataatccact agcaggtgtt catttccagc
     3361 agaagtatga aaaattagct tttgaattag gtggaaaaaa ttataatgca ccagttcagc
     3421 ttgttggaga ttttataaac aataagactt ctttgaattt aggaagtgtt gagccatcat
     3481 atagaccagg atatacactt acaaatttaa gtgaatgttt gcctagcttc gtaacagaga
     3541 ctatgagaga agcgtttata tctcttgata aaaaattgaa tgggtttgca atgtatgatg
     3601 ctgttttgac gggagtagaa actaggtcat catcaccaat aagaatagtg agagatgaaa
     3661 atactttaga atcagtgagt attaagaatc tatatccatg tggtgaaggt gctggatatg
     3721 caggtggtat agttacagca gcagttgatg gtataaaatg tgcagaaaaa ataatacaaa
     3781 aatacaccaa taatacaaaa atttaacata aacttaatga ttttttaata tttaacatga
     3841 atttaacatt atcactccta taatttgata aaatacaata ggttataaaa tttttagata
     3901 cagtaatctc tttacaagga ggaaataatt tgaacattat aataagtcta atgggtggat
     3961 taggtctgtt cttatatggt atgaacctta tgggagaagg tcttcaaaaa tctgctggga
     4021 caaagctaaa aaagataata aaactactta ctagtaacct atttatggga gttttggtag
     4081 gtacaggagt tacagcagta atacaaagtt caagtgctac tactgtaatg gttgttggtt
     4141 tcgtaaatgc aggaataatg actttaaaac aggcaatagg agttataatg ggtgctaata
     4201 tagggactac tgttacagcc caattggtat ctttcgactt aactggtatg gccccagtag
     4261 cattaggtgt aggtattata ctttatttat ttggaaataa gcctagaata aaaaatatag
     4321 ccgaaatact tataggtttt ggtatattat ttacaggtat ggattttatg aagatggcag
     4381 tggagccact aagagactat caaggattta ctgacttact tgttacattt ggtagatatc
     4441 cactacttgg attattattg ggatttggga ttacagcaat aatacaaagt tcaagtgcat
     4501 ctatgggtat gttggttgca ctggcagcag aaggtctagt gccactatca gcagcactgc
     4561 caattcttta tggccaaaat attggtacat gtgtaacttc actgttatca agtattggtg
     4621 ctaacaaaaa tgcacgaaga gcagctatga tgcatctaat cttcaatgtt ttaggaacgg
     4681 tgatattctt aatattcttg aataaaccag tagtaagcat ggttacaagt tgggatccaa
     4741 gcaatgttgc aagacaaata gccaatacac atacattgtt taatataata tctgtattga
     4801 tacttttacc atttacaagt cttattataa aactagctat aaagcttgtt cctgatagag
     4861 caggtgatat agatgaagat gaaactaaaa ctattaaata tatagatgat agaatgatag
     4921 aaactccttc tatagctctt gcaaatacga taaaagaagc acttcgtatg ggtgagaagg
     4981 cgaaggaaag cctaaatgca tctatggaag ctttagtaga acattcaact gaaaaaatag
     5041 ataagacata tagacgagag agattaatca atgatttaca gaaagctatt ttaaattatt
     5101 tacttaaact ttcaaaagca gctttagatg atgattcaag agaagttgtg gatactttgt
     5161 ttaatacagt caatgatata gaaagaattg gtgaccacgc taaaaatata gctgaattat
     5221 cacaagttgc aatagattca aatatttatt tctcagaaga aggacaatct gaacttgatg
     5281 ttatgtataa tagagttgtt tcagcatata cttatgcatt agaatcaatg aggagcgata
     5341 atgttgattt agcttgcaag gttataaaaa ttgaagaaca agttgatata atggagaaat
     5401 cttgtagagc taatcacatg tatagattga ataataatct atgtagtata gaaaatggag
     5461 ttatttattt agatgtaata agtaatttag agagaatatc tgaccatgct gtcaatatag
     5521 cacaacaagt tatagcgaag agattaggaa atgattaaaa ataattaatt taaaataata
     5581 taaaatttaa aatagaggat aaataaaaga gaaactttaa atgatacgtt tagagtttct
     5641 ttttttgtaa atttatttga ctaaaatttt taaatttata catagttata gtatttagaa
     5701 gaatcgattt cctgataata aatgatgaga tatgtatgat tttgtggcaa acttttaaaa
     5761 aaaacaccta tattttgtgt tataatctct ttaaaacgaa tatgctgaaa agagggattt
     5821 aaatgggaaa aataaatttt gattatagta aagctacaga gtttttttgt caaaacgaaa
     5881 tagatgtcat gcaaccatat gtagatgttg cgcacgacat gttacataat aaaactggtt
     5941 taggaaatac ttttttagga tggatagacc ttcctaaaaa ttatgataaa gaagagtttg
     6001 acagaataaa aaaatctgct gataagataa aatcagactc agatgttctt ttggtaatag
     6061 gtataggagg ctcttattta ggttcgagag ctgctataga tatggtaagt cattctttta
     6121 ggaatggact taaaaaagag caaagaaagg ctccagaagt atattttgta gggcataata
     6181 taagctcaac atatattatg gatttattag atataattga aggaaaggat atttctgtaa
     6241 atgtaatatc taaatcagga actactacag aaccagctct ggcttttaga atatttaaag
     6301 attacttaga gaaaaagtat ggtaaggaag aagcaagaaa gagaatttat gcaacaacag
     6361 atgcaagtaa aggtgcactt agacagttag ctacagaaga aggatatgag acttttgtaa
     6421 taccagatga tgtaggtggt cgtttttctg tattgacagc agttggatta cttcctattg
     6481 ctgctgctgg acttgatatt gatgctatga tgaaaggcgc aaatgatgct agagaagcat
     6541 ttcaaaatcc tgacttaaaa agtaatgata gttatagata tgctgttgct agaacaattt
     6601 tacatagaaa aggaaaagat gtagagttat tagtgaacta tgaacctcaa ctacattatg
     6661 taagtgagtg gtggaaacag ctttatggag aaagtgaagg aaaagaaaat aaaggtttat
     6721 tccctgcttc tgtagatttc tcaacagatt tacattctat gggtcaatac atacaagatg
     6781 gaaaaaggct tttatttgag acagtactta atgttgaaaa ctgtaagaga aatataacta
     6841 taagttcaga ggaagtagac ttagatggtt taaattattt agcaggtaag actgtagatt
     6901 ttgtaaatca taaagctttt gaaggaactc tattggctca tacagatgga aaagtaccta
     6961 atttagtaat aaatatacct caactagatg agtataattt tggatattta gtttatttct
     7021 ttgagaaggc ttgtggaata agtggatatt tattgggagt taatccattt gaccaaccag
     7081 gagtagaagc ttacaagaaa aatatgtttg ctttactagg caaaccaggt tatgaaaaag
     7141 aaaaggaaga gctagaaaaa agacttaaat aattttaaat agttaaggtt taattaagat
     7201 taaaataata taatgaaatc atcaaaaagg agtttactca aaatagatga tttcattttt
     7261 tttatgaaaa attgggaaaa atatattttg ataatttata ttatattaag atagggagtg
     7321 gtatgagatg tcaagaagaa agaaagggat aagtttagta atattagttg ctataataag
     7381 ttcaatactt agtagttttc taacaataat tttagttaag gacaatttag taagcaaatc
     7441 tacaggaagt tctacgccga tagttgtaaa tgatgatggt aagagtcaaa atatttatca
     7501 agcagttgca gaaaaagcta cgccatctgt tgtaggtatt acaacaacta gtgttgatac
     7561 tagtaatatg tttgcaatac caactgaaac acaaggtgta ggaactggaa tcatagttga
     7621 ttcaaatgga tatatactta ctaattctca tgttatatca gatggtcaag ctacaagtgt
     7681 aaatgtatta ttcaatgatg gttcaacaac ttcaggtaaa gtagtatggt ttgaccaaca
     7741 acttgattta gctatagtta aggttgataa aactggacta acaccagcag aatttgctga
     7801 tagtgacaaa gtaaaagttg gagatatatc aatagcaata ggtaatcctc ttggattaga
     7861 ttttcaaaaa acagttactc aagggataat aagtggatta gatagaacta tacaaacaga
     7921 aaaaactaat atgactggtt tattacaaac tgatgcaagt ataaatgcag gaaatagtgg
     7981 aggaccattg ttaaatcaaa aaggtcaagt aattggtata aacacagcaa aagcttctca
     8041 agcagaagga ttaggatttg caataccaat caatacagct aagagtatag ttgaagaagt
     8101 aatcaaaaat ggaaaatatg aaaaagtaac tttaggcata aaaggtacag atgtatcaaa
     8161 ttatgaagca gcaacaggta caaaattaag tactgataaa ggtgtttatg ttgcagaagt
     8221 aatatctggt tcatcagcag aaaaagctgg agtaaaggta ggggatatta taactaaagt
     8281 aggagataca gatataacag gtatgaatga tttaaataaa aagttgtata ctttctcaaa
     8341 aggtgctagt acaaaaataa cagtaaatag aggcggaaaa gcagtaacaa taaatgtgaa
     8401 tttctaaata tgaccaaaaa gatagagact acatggtaaa gtgtagtctc tatttttatt
     8461 taatttttat agtatataca tctaaattaa ttaaaatgat gtattgttta tagtttattg
     8521 ctatattttc ttataaaaat aaatttaata aatatttaaa tttaatttac aaaaacaata
     8581 taaatatcat aataaaataa aattttttaa tattactaaa aatatataga caaaataata
     8641 acaaatatag tattatgtaa ttgtagaaat gcttttcata ggtatattct atgaagatga
     8701 attgcataaa aatgaaaaaa tggaatggat attatttagg ggtgaaaaat catgaatcca
     8761 ttagttataa acttacaaaa atgtagcata catgatggac cagggataag aagtacagta
     8821 ttttttaaag ggtgtccact tgaatgtgtt tggtgtcata atccagagag tcaaacatat
     8881 acaaaacaag ttttgtataa tgaagaaaga tgttcaaaat gtgaagcatg tataaatata
     8941 tgccctcata aggcaattta caaaggtgaa acaaaaatat gcttagacca agataagtgt
     9001 gagttttgtg aaacttgctt agactattgt gtaaacaatg caagagagat tgttggtcaa
     9061 gagtattctg taagagattt ggttaaagag atagagaagg atagaatttt ctatgaagaa
     9121 tcaggtggtg gagtaactct atcaggtgga gaagtaatgg ctcaagatat ggacttcatt
     9181 tgtggagtaa taaacatgtg caaatcaaag ggaatccatg tagcaattga tacatgtggc
     9241 tatgcaaagt cagaaaatta tgaaagagtt gctaaatgtg cagatttgtt tttatatgat
     9301 attaagctta ttgatgaaga taagcacata aaatttacag gaaaatcaaa tgacttaata
     9361 ctgaaaaatg ttaagatttt aagtgaatta ggggctaaca taaatataag aataccatta
     9421 attgtaggag taaatgtaga tgatgaaaat ttagaggtta aaaaaatgat agagttttta
     9481 aagcctttga atatacaagc agtaagtctt cttccatatc ataatattgg taaacacaag
     9541 tatgataaaa tttataaaaa gtatgaagga gaagaactac aaagaccatc agaagaaaaa
     9601 ttagaggaaa taaaaagact gtttgaagca agtaatttca atactaagat tggagggtaa
     9661 taaaatggca agaggaactt ttgagagaac taaaaaatta agagaagaaa gtattaatgc
     9721 agaaccacac atatctattg aaagagcagt acttatgaca gaagcttata aaaaatatga
     9781 gggaagtgta gagattcctg tacttcgtgc tttatcattt aagcattata ttgagaatag
     9841 aactcttagc ataaatgatg gagaattaat agtaggtgaa aaaggtgatt ctcctaatgg
     9901 agcaccaaca tatccagaaa tttgttgtca tacaatggaa gatttagaag taatgcacaa
     9961 tagagatata ataaatttta gcgtatcaga agaagctaga aaaatacata aagaagaaat
    10021 aattcctttt tggaaaaaga gacaaacaag agataagata ataaatgcaa tgacacctga
    10081 gtggttagca gcatatgaag caggtatgtt tacagagttt atggaacaaa gagccccagg
    10141 acatacagtt tgtggagata caatatataa aaaaggattt ttagatttaa agaaagacat
    10201 agaagcaaga ttaaaagagc tagattttct taatgattta gatgcatata ataaaaaagc
    10261 agatttagaa gctatggcaa tagcttgtga tgcaatggtt atattaggta aaagatatgc
    10321 tgagaaagca agacaaatgg cagaagaaga aacagatgaa gctaagaaac aagatttatt
    10381 actaattgct gaaacttgtg atgttgtacc agctcacaaa ccagaaactt atcatcaagc
    10441 tatacaaatg tattggtttg ttcatatagg ggttactact gaacttaata tatgggatgc
    10501 ttttactcca ggaagacttg accaacatct aaatcctttc tatgaaagag atgtagaaaa
    10561 tggaatacta gatagagata gagcacaaga attacttgaa tgtttatggg ttaaatttaa
    10621 caatcagcca gcaccaccaa aagttggtat aacattaaaa gaaagtagca catatacaga
    10681 ttttgcaaat ataaacactg gtggaataaa tccagatggt caagatggtg ttaatgaagt
    10741 tagttatata atacttgatg ttatggatga aatgaaattg atacaaccta gttcaaatgt
    10801 tcaaataagt aaaaagactc ctcaaaaatt cttaaagaga gcttgtgaaa tatcaagaaa
    10861 aggatggggt caaccagcat tttataatac tgaagcaata gttcaagagc ttatggaagc
    10921 tggaaaaact atagaagatg caagacttgg tggaaccagt ggttgtgttg aaactggatg
    10981 ttttggtaaa gaagcatatg ttctaactgg atatatgaac attccaaaaa tacttgaatt
    11041 aacattaaat aatggatatg acccaattag taaaaaacaa ataggtattg aaactggaga
    11101 ccctagaaat ttccaatctt atgaggaact atttgaagca tttaaaaagc aattacatta
    11161 tatgattgac ataaaaatag aaggaaatgc tgtgatagaa aatatatgtg caaaacacat
    11221 gccttgtcca cttatgtcta caatagttga tgattgtata gaaaaaggca aggattacca
    11281 aagaggtgga gcaagataca acactagata tatacaaggt gttggtatag gaactataac
    11341 agatagttta acagcaataa aatataatgt atttgataag aaaaaattcg atatggatac
    11401 attgcttaaa gcacttgatg ctaattttga aggatatgaa gctatactta atttagtttc
    11461 aaataagact cctaaatatg gaaatgatga tgattatgct gatgaaataa tgcaagagat
    11521 attcaatgca tattacaatg aggtaactgg tagaccaact gtttgtggtg gagaatacag
    11581 agtagatatg ttaccaacaa cttgtcatat atactttgga gaaattatgg gagcaagtcc
    11641 aaatggtaga ctttgtgcaa aaccagtttc agagggaatt tcacctgaaa aaggtggaga
    11701 tactaatgga cctacagcag ttataaaatc ttgtgctaag atggaccaca taaaaactgg
    11761 tggaacatta ttaaatcaaa gatttgcacc atcagtagtt caaggagaaa aagggttaga
    11821 taatatggct aatctagtaa gagcatactt taatatggat ggtcatcata tacaatttaa
    11881 tgtatttgat aaaaatgtac tgttagaggc tcaaaagaat cctcaggatt ataaagactt
    11941 aatagttaga gttgcaggat atagtgacca tttcaataat ttaagtagaa cactacaaga
    12001 tgagataata ggaagaactg agcaaacatt ctaggtttgc ttgtaaataa aacttcttac
    12061 tatataaaat cgtaactgat ataataaagt ataaatggtt tgattccatt tttaaaattt
    12121 tagattaatt atgagctaac tttctaaata ggaagttagc tcgttaagtt taatgaaaaa
    12181 atcgttaaat ttatatttag taaatcatac acatattagt aagtaataca tacaaaagaa
    12241 taatttatta tagttataat aaggttatat tttataaaaa taagataatt ttaagcagtt
    12301 aaaataaagt caatttagct actttaaaat aaaactaata gaaaaaagga gatttgatat
    12361 gaaaacttta ggatttattg gttcagggaa tatgggaagt gcaatgatag gtggcatagt
    12421 acagtcagga ttagtaaagg cagaagatat aactgtatca gatttaagcc aagttgcctt
    12481 agataaagta aaagaagaat ttggagtaaa tactacaact gattcaaagg tagctgttac
    12541 aaattctgat gtggtaatag tagcacttaa acctaatata tgcgaaaagg ctttaactcc
    12601 acttaaagaa ttaatagatg caaataaaat aatagtatct atagcagcag gtaaaactat
    12661 agaaagtctg gaaagtttta taggttcaga taaaaaaata gtaagagtaa tgcctaatac
    12721 tccagccctt gttggcgaag gaatgtcagc tctttgtaaa aatagtaatg taacagatga
    12781 agagttaaat atgattaagg ctctgtttga atcttttggt gaggcagaaa tagtatcaga
    12841 atatttaatg gatacagtta caggtattag tggttcttct cctgcatatg tatttatgtt
    12901 tatagaagct atggcagatg cagcagtatt agctggaatg ccaagacaac aagcttataa
    12961 atttgcatct caagcagtaa tggggtcagc taagatggtt cttgaaactg gaaaacatcc
    13021 aggtgaatta aaagatatgg tttgttctcc agcaggaact actatagaag ctgttaaggt
    13081 attagaagaa gaaggcttta gagcagcagt tataaaatct attgttgctt gtatagataa
    13141 gtctaaagat atgagtaaat aacagatgta attaagttta ctatatggtt tcttgaattg
    13201 tttttttagt gcttatttat atctatacat aaatgtattt ggtataagta aaataaga
//
LOCUS       DACULQ010000112        13235 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.112, whole genome shotgun sequence.
ACCESSION   DACULQ010000112 DACULQ010000000
VERSION     DACULQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.112"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            146..1564
                     /locus_tag="JA154_12670"
     CDS             146..1564
                     /locus_tag="JA154_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAT7996701.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            1640..2452
                     /locus_tag="JA154_12675"
     CDS             1640..2452
                     /locus_tag="JA154_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7996702.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            2875..3492
                     /locus_tag="JA154_12680"
     CDS             2875..3492
                     /locus_tag="JA154_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996703.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(3649..4101)
                     /locus_tag="JA154_12685"
     CDS             complement(3649..4101)
                     /locus_tag="JA154_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996704.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRIFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            4549..5907
                     /locus_tag="JA154_12690"
     CDS             4549..5907
                     /locus_tag="JA154_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT7996705.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDGEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGLLRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            6180..6560
                     /locus_tag="JA154_12695"
     CDS             6180..6560
                     /locus_tag="JA154_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAT7996706.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMRQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            6685..6885
                     /locus_tag="JA154_12700"
     CDS             6685..6885
                     /locus_tag="JA154_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996707.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            7081..7809
                     /locus_tag="JA154_12705"
     CDS             7081..7809
                     /locus_tag="JA154_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996708.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            7831..8748
                     /locus_tag="JA154_12710"
     CDS             7831..8748
                     /locus_tag="JA154_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996709.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTETGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            8890..10074
                     /locus_tag="JA154_12715"
     CDS             8890..10074
                     /locus_tag="JA154_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7996710.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPLNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGLNQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLSVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            10181..10369
                     /locus_tag="JA154_12720"
     CDS             10181..10369
                     /locus_tag="JA154_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAT7996711.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            10519..11169
                     /locus_tag="JA154_12725"
     CDS             10519..11169
                     /locus_tag="JA154_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAT7996712.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKTKEAVEKYREYFADKGIFENKIYENIKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            11272..12210
                     /locus_tag="JA154_12730"
     CDS             11272..12210
                     /locus_tag="JA154_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HAT7996713.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            12236..13087
                     /locus_tag="JA154_12735"
     CDS             12236..13087
                     /locus_tag="JA154_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAT7996714.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGTL
                     IKSDTIESKSERIKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         5272 a         1276 c         2344 g         4343 t
ORIGIN      
        1 cactcacatt ttaatgtatt gtgaaatttt tgttgaactt tatgtaccat aacttataaa
       61 atttatgtca tgttatattt gaataagtaa taaaattgta gatgtaaatt aatctacaaa
      121 atttatatga aatggagttg atgctatgac agaattaatg attataagtg ggttagtact
      181 attgatttgt ataacttcta gcaaggtatt gtataaattt ggagtaccaa tattattgat
      241 ttttatctta ctagggatgt tatttgggtc tgatgggatg gtaggaatct attttgataa
      301 ctatgaactg acaaaacaat tatgttctat tggactaata tttataatgt tttttggagg
      361 gtttggaaca aactggaata tggctaaacc tgttgctata ccttctatat taatgtcttc
      421 actgggtgta ataattacgg ctggggttac aggtatgttt tgccacatgg taataggaac
      481 tagtttactt gaaggtttat taattggggc aattgcagct tcaacagatg ctgcatctgt
      541 gtttgctgtg ttgcgttctc aaaaactaaa cttaaagggt tcacttgcat cattattaga
      601 agttgaaagt ggaagtaatg acccaatagc gtatatgttg acactaattc ttttgacatt
      661 gatgaataat tctggggtag aacttattgc accaatggta ataaaacaaa tagcttttgg
      721 gttagctata ggatttatac tagcaaaatt atctgtttat attttaagac attcaaactt
      781 cgagatagaa ggattttata ctatatttat aacagctatt gcaatacttt cttatgcatt
      841 tagcgagtat cttggaggaa atggatattt aagtgtttac atttcaggta ttattatagg
      901 aaatagtagg atacctctta aaaaaagttt ggttcatttc tttgatggaa tttcatggat
      961 tatgcaaata atgttattct ttatgttagg attgttatct ttcccttcta agttaccaaa
     1021 tgtatttggt atagcaatgg ccatatctat attcatgata gttattgcaa gaccattagc
     1081 aacatttata gttctagcaa aatttaaatt ctcaaataaa gaaaaattat tcatatcgtg
     1141 ggtaggtctt agaggtgcgg cttcagtagt ttttgcaatt tatgctgtta ctcaaggagt
     1201 agccatagag aatgacatat tccatataat attttttata gcgttgcttt cagtaggaat
     1261 acaaggttca ttgataccaa ctgtagccag aaagttggat ttagttgatg ataatacacc
     1321 tgtattaaaa acatttaatg attatgatgg agaaattagc tctaaactta tcgaagtaaa
     1381 tgttgatgag ggtaataaat gggctaataa gagtataata gattctaata taccagacga
     1441 tatattaata gttatgataa aaagaaagga acaggtttta atacctaaag gttcaaccat
     1501 tataaaaaca ggggatattt tggtattgag tggagataga attgaagaat taatacaatt
     1561 ataatttatt ttgtattgac tttaacctaa ggggaaagtt taaacttaaa ttaatcaaat
     1621 ctaatgcgca ggtgatgaga tgttttctat aggaatgttt tctaaaataa ataaaataac
     1681 tacaaagaca ttaaggtatt atgaagatat agggttgtta aaaccagaat atgtagatga
     1741 atttacaaaa tataggtatt atacaacaga gcaattacct aaattacatc agataataac
     1801 tttaaaacaa atgggtttat cattagctga gataaaaaaa gctgtagaaa atcctgaaga
     1861 agttgaaaat attttaatga ataaagaaaa agaaatgttg gatgtaatta aaagtgaaga
     1921 atataaaata attaaactac gtaattacct tatgaatttg aaaggagaag tcaatatgaa
     1981 tgatgtaatt attaagtctt taccaaaagt taaagttgct tctatgagaa aagtgttaaa
     2041 ggggtatgat gaacttttta atctatttcc aaatgtaatg gctgaagaaa tgatgaaaac
     2101 agggtgtaca tgtgcagttc ctgattattg ttttaatata tatcatgatg gtgaatatag
     2161 agagactgat atggatgttg aaatgtgtca agctataaat gatttaaagg aagatacgga
     2221 tatattaaaa tttaaagaat tagatgcagt tgaaaatgca gtttgtatat tacacaatgg
     2281 tccatattca acattaggaa aaacttacgc agatgcattt aaatggatag aggacaataa
     2341 atataaagtg gttggaaatc ctagagaatc ttatatagat ggtatatgga ataaagagag
     2401 tgaagaagac tggttgacag aagttcagat accagtgaaa cgcatgttat aatatgtcta
     2461 caaagtataa ataagaataa aaaaggtggt gtctcaaagt aaacatttta gtttatgaga
     2521 cactcttttt catatgaaat caagtatatt tttataatta cgattatgaa aaaaggctta
     2581 tctatatttt tagacagcct cttatttttt tattataata gttttctaaa gatgctctat
     2641 ttataaattt ttattaaaga ctttataaat aattgtattt aatatacaaa atatatataa
     2701 atattgatgt tttatatata aagttgtata tttttttaag gatataaagt aaaaaaatat
     2761 acaatatcaa gaaaaaaaga tattgggtat ttaaagtaat actttattta attttaagta
     2821 aatcgggtat ctacatatat agatactaat ttcaaagaat aggagggtgt ttatatggaa
     2881 atattcgcaa taggacattc aaattatcca tatgataagc ttattaatat gataaaaaaa
     2941 tatgacataa attgtgtagt tgacattaga gaaactccat attctaaata taatattcaa
     3001 tacaataaag aggcatttaa cgaatctctt agaaattccg gatttttata tatatacatg
     3061 ggaaaagaat ttggagctaa aagaactaat aaagatgtat atacgcaaga aggttatgca
     3121 gattttgaga aagtagcaaa agaagatatt tttttaaacg gaatagaaag attaaaaaag
     3181 ggttgtcaaa tgggttatag gatagtttta ttaggagcta tgcaagaacc tataagatgt
     3241 catagaagta tcttggtggg aaaagtatta aataaagaag gttttgatgt aaaatatata
     3301 atgcatgaag gaaatttggc atatcaggaa gatatagagg aatcattatt ggataagtat
     3361 tttagtgata gaaagcaact gagtattgac aatttattag ggagtgcatt aacgagagaa
     3421 gaaatgatac aagaaggtta taagctagct aataaagaaa taggatatag aactgagaaa
     3481 ctaggtaaat aaaaatatac aagtagataa aatacagtac ttatcttaag taaaataata
     3541 ttatgaattt ataatagcta ggcatgatgt gccaattaaa aatattctag ttatttaata
     3601 atatcattaa atttagggta ttttttaata gcacatcatg ttgcagtttt aaaaaataaa
     3661 atctctaata ttgccataat caactgttct attaagagta ggatgactag atataaatct
     3721 aactgtaatt tttgaattta acaaacaagg atttgaaatt aaaaatattc tatctccatt
     3781 gacaatagaa gtaatagcta ctggcaaaat atctacaaat gtatcatatc tgtttttagc
     3841 aatttgataa ggataatttc ctacaaaaac aaatagattt aaatcatata tattatcagt
     3901 aaaccaagcc cattgaccat aaactttgtc gatattcatt gtgcctaaac caagtgtatc
     3961 aaagtatgtt gctatagtta aataaatatt ttccatgttt tctaaatatg aaacagtata
     4021 tcttctgaaa cgtatgggtg ctgtggaaga aataggataa accatattta catttagatt
     4081 atttgcattt aaattaacca aagtatcaca tcctaaaatt agtctataaa atacaatatt
     4141 taaatggatg cgattttgtt acatctaaag tgtaatattt tatatattga aaaagtggta
     4201 aaagtttgga tgattaaata atattataaa aaatatataa aatgtactta ttgttattgc
     4261 tacacgatag tgatacaata ttaacgaaat atagctactt ttaataataa cggaaaatat
     4321 ttatattaaa ttaataaata ttttaaatgt ggtatccata aaaaataata aacataagat
     4381 aattataaag atatactgtg ttcaaatgat attaaacaag atttatatta tttgaaagca
     4441 gtatattata atgcgaacaa tacaattcta aactttgcat tgtcaattta cagttcgata
     4501 aactaattaa aaattaaatt tatataaaga gggaaaagag gaatagggat gtttataagg
     4561 aaaaatgtta taaagttagc aattcctata atgatagagc agacgtttgt catgctttta
     4621 ggagtattta atacaatgat ggcaggtcat attggggaag aagctgtatc tgcagttgga
     4681 atggttgatt caataaacaa tatatttata tctgtatttg ctgcactatc tgttggagct
     4741 actgttgttg tagctcaaca aataggaaaa aaagatggtg aaaaggtgaa tgaaactatt
     4801 aagcaggctc tagtgtctgg agtaatatta tcctttacaa taacaatatt gatgtgggtt
     4861 tttagaactg gtgtaattaa tttattttat ggttccgcag aagaattggt aaaatcaaat
     4921 gcaaaattat acatagagtt tacattattt acgtatccat ttattgcgac acaacaaata
     4981 gcaaatggtc ttttaagagg gtgtggagat gtaaagaaac ctatgtatgt gactatgttt
     5041 atgaatatag taaatgtaac attaggatat atacttatat atggtattga tttaaatcct
     5101 ctaggaataa atttgcaaac accatcctat ggcataacag gtgcagctat atctattgct
     5161 atagctagaa taattggatg tatagtaatt ataagagctt tatttaaagg aaacgaattt
     5221 atatctatgg gtaaaatatt tccatttaag attgatgttg aaactcaaaa gtctatattt
     5281 aatataggaa taccagctgg agttgaacaa ctacttttta atgcaggtaa attgattgtt
     5341 caagtattta tagttactat gggaacatca gcaattgcat ccaatgcaat aggaatgtca
     5401 attgcatcca taataaatgt aattgggaat gctttagctt tgtctgctac aaccttggtg
     5461 ggacagtata taggacgtgg agatattaaa ggagctaaga gtactttatt atatttaact
     5521 aaatttgcaa caatttgttt atttatagta ggagctatat ttataccaat tgcaccttgg
     5581 atttcaagta tgtatactca aaatgctagt gtaattgaca tctcaacaca attaattcga
     5641 agtgattgtt tagctatggt aatatggcct atatcctttg tattatcagc agggctaaag
     5701 ggagccggtg atactagata tacaatgatg actgcagtaa taggtatgtg gatttttaga
     5761 atttttacag gttacgtatt gggggttgta ttagaatttg gagtgttagg aatttggata
     5821 ggtatgtata ctgattggct tgttagaggg actttatatt gtttaagatt aagaggaaca
     5881 aagtggctaa aacacaaagt tgcatagaat atatgaaatc acttttagat ggataactct
     5941 atttagaagt gattttttat gtagttatta tcataattat aagtatgata tataaggtga
     6001 ttaaattaca taaataaagc ctataatagt atggatacta taaattatat attgagcaga
     6061 attacataat ttggcgataa atattagaaa tatatatcta tagaaaataa tatatatggg
     6121 tatattatta aatataaaaa atattaggta tttatttaat aataaaggtg ggtattgtta
     6181 tgataaataa aataggaaaa ataactctct atgtaaataa tcaagaggat gcaaagttgt
     6241 tttggacaga aaaattgaac tttgtagtaa cttatgaaaa ggaaatgggt ccaaatatga
     6301 aatgggtgga agtagcacca agtaaaaatg aatttacaac atttgtgttg tatgataaaa
     6361 atctaatgat gagacaaaac aaagagataa atgtatctca tccatcaata attttaagta
     6421 caagagatat taatggaact tatgaacaaa tggcaaataa cgaggttgag gttggggaat
     6481 taatggagat gccatatgga aggatgtttt cttttaaaga ccaagatgga aatgattatt
     6541 tggtcagaga ggataaataa ttttacaaag tattaaattt actcaatatc tgatataatt
     6601 agagacaata attattataa actagtctag gattagaatt atataattaa aaattaagtg
     6661 taaattttga gtagaggtga taaaatgttt aagaaaatgg cagtactaaa agatatagca
     6721 actaaaatag gtcgtaaaaa agcttatgaa ctattagaaa tggttgaagg taatgatgcc
     6781 tttgtagctg aggtgaagat aaaaaagaat ggaatagaat ctaaaaaaga agaaattatg
     6841 ttaaaagata atcaaaaaat aatattagag tatatagaag gctaattttt aaatacatat
     6901 aagttgatat actaaatttc tgagatgagt ttacataaaa ctcatctttt ttacttttca
     6961 ataactttgt tttattattt aatatacatt ttatctgctt agagttgctg atatataaaa
     7021 attactgata taataaaaga atggatatta atgtaatatt ttatagaaaa gaggggtatt
     7081 atgcttgaaa taaaaaatct ttcctttaat gttgagtcta acaatgagga actaggaata
     7141 ataaatgatg ttagcctttc atttgagagg ggaaaactta ttgttatcac agggccaaat
     7201 ggtggaggaa aatctacaat tgctaagctt attatgggaa ttgaaaaagc tacgagtggt
     7261 cagataattt tagatggaga agatataaca aatcttagta ttacagaaag agcaaaaaaa
     7321 ggaataggat atgcatttca acaaccacca agaataaaag gtatgacagt tgaaaattta
     7381 cttacacttg ctcatggaaa accattgtct actgatgtat gttgccaata tcttacagat
     7441 gttggattgt gttcaaaaga ctatttaaat cgtgaagttg ataactctct ttctggaggg
     7501 gaaatgaaaa gaattgagat agcaactctt tttgctagag atttaaaggt ttctatattt
     7561 gatgaacctg aagcagggat agacctttgg agctttggga aactcaatga aagttttaag
     7621 aaaatacatg aagagtctaa ccagactata atcataattt ctcaccaaga aagaatatta
     7681 gagttagctg atgaaataat tgttctacaa gatggttcag taaaaagcca tggaacaaaa
     7741 gaggctatat tacctgaaat catgtgtcaa gttaattcaa gttgtgaatt aatgaaagac
     7801 atgaattaat tagaaaggaa ggcaaattaa atgaacaaaa tagatttaaa tcttttagaa
     7861 aaagttgctg aacttcgtgg aactccatta ggagcataca atatacgtaa aaatgggcaa
     7921 ggagtagaga gacattcaac tgaaaatgta actataattc ctaaaactga taaacctgga
     7981 atagatatta tagtaaagcc aaatacaaaa ggagaaaatg tacatatacc agtaatagta
     8041 acagagactg gtgtacagga tatggtatat aatgattttg aaattggaga aaatgcagat
     8101 gttgttataa ttgctgggtg tggtatacat aactgtggtt gtgatgatag tgaacataat
     8161 ggtatacata gatttttctt acgtaaagga gctcgcctaa aatatgtaga aaaacattat
     8221 ggtgaaggtg atggagaagg aaagagaatt ttaaatccag ttactattgt caatatggat
     8281 gaggattcat attgtgaaat ggaaacaatt caaattaagg gtgtagattc aactaaaaga
     8341 gaaatggatg caaacctagg taaaggagct aaaatagttg tatttgagaa attattaact
     8401 catggtaatc aagttgcaga atctaatatg aaggttatct tagaggggga aaactcttct
     8461 gctcaagtag tttctcgtac agttgggcaa gataattcta agcaaaagtt taatccttgt
     8521 gttattgggg attccctttg ttctgcacat gttcaatgtg attcaataat tatgggaaat
     8581 tctcaaatta gtgctatacc tgaaattgct gcaaatcatc cagatgctct tttagtacat
     8641 gaagcagcta ttggaaagat tgcaggagat caaatactaa agatgatgac attaggtctt
     8701 actgaagaag aagccgaaca acagatactt aattgcttct taaagtaaat taaagatttt
     8761 atttaattta atataattaa tatagtataa tatagtgaat aagtctaaaa tatttttatt
     8821 tttaggctta ttttctttat gtaagaaaag ctattagaga aattatattg taattaggat
     8881 gtggaataaa tgaaaaattt gcttataaga aatgggacaa taatatctcc aacagatggt
     8941 atcaaatttg aaaaaagaga tatcttggtt ttacatggga aaatatatgc tattggattg
     9001 aaccttaaag agaaaataag aaaagaaaaa ttacttacaa ataaagagtt cgagataata
     9061 aatgccgata atatgtatat ttccccagga tttatagatg ttcacaccca ttgttataag
     9121 agaaaattac ctacaggtat ggattctgat tcaattggaa ttgaaaaagg ttctacagtt
     9181 atatttgatg caggtacagc tggtccttta aactactatg attttaaaaa aaagtatatg
     9241 gatgagtgta aaacagaagt atattctttt ttaaatgtta caaatgaggg actaaatata
     9301 ttgaatgaat caacttcttt agatgttata aattttgaca aggttgagtc agttgtagaa
     9361 aaaaatatag aaaagataaa gggtataaaa gtaaaagcga gtaaatttca atctaatgaa
     9421 agtggtatag atataattaa gaaatcaaag caagttgcca aaaaattaaa tttaccctta
     9481 gtggtatata taggagagta tccatcatat atagatgaag taatgaatat tttgggaaaa
     9541 ggagatgtgg ttacaccagt atatggaaat agtacaaatg gaatccttaa agaatctggc
     9601 acattaagaa aatcagtaat aaatgcaaag aacagaggag tattgtttga tgtatgtcat
     9661 ggattgaacc aatttgattt taaagttttt aaaaaagcaa taaagcaagg tttagagcca
     9721 gacctaataa gtactgatat gcatataaaa aacatgaaag acgtaaacta ttctctttta
     9781 agtgttataa ataaaattat ggaacttggg atatccttag aaaagtgtat agaaaaagta
     9841 actgaatacc caacaaagat tttcgattta catggaaata aaggcagaat aaagattggt
     9901 ggagaaggtg attttacaat attcacaatg gaggagtgta atgatattat aaaagactac
     9961 aataataatg aacttgtttt aaataaaaaa ttaagattgc aatatactgt aaaatcatgg
    10021 atgaaaagca gtgaagttta tagacatgga tacgaaaata ctataataaa ctaaacaata
    10081 gtgataaaat aataaatata tgtttaaatt ggtatatatt ggataaatat ctaaggaaac
    10141 aacaattaaa aaataataat tttaggggag gtattttagt atgaaagcat ttgttgataa
    10201 ggatgtatgt gtagcttgtg gagcatgtat aggaacatgt ccagacgttt ttagtatgag
    10261 cgatgaaggg cattcagtag caattgaagg agaaatacca gcagacttac aagatttatg
    10321 taaagaagcc gaagaaggat gtccagtttc agctataaca gttaaataga gctgtttaaa
    10381 tttattttta aaataaatat ataagattta attgggtact taagctatag tattagaaat
    10441 ttctattact atagcttttt aaaatagata aacttaatat ataagatatt actttttaca
    10501 aatatggagg actgaaatat gaaaaaaaat tatgaaatag tattattcga tttagatgga
    10561 acacttacag accctaaaga aggaataaca aagtctattc agtattcttt aaatagcttt
    10621 ggcataaaag aagatttgga aaacttagac caatttatag gaccaccatt acatgatact
    10681 tttaaggagt attataaatt tgaagataaa aaaactaaag aagctgttga aaagtataga
    10741 gaatattttg ctgataaagg gatatttgaa aataaaatct atgagaatat aaaagagata
    10801 ttagaaatgc tctataaaaa tggtaaaata ttattagtag caacttctaa gccaacagtt
    10861 tttgcagaaa ctatacttag atattttgat atagatagat attttaaata tatagcaggt
    10921 agcaatcttg atggtacaag agtgaataaa aatgaagtta tacaatatgt actagaccta
    10981 tgtaatgtaa aagataaaga taaagttatt atggttggag atagaaagta tgacataatt
    11041 ggagccaaaa aaataggtat agattctatt ggagtgttgt atgggtatgg ttcttttgaa
    11101 gaacttagtg aaagtgaacc tacttatatt gtcgaaaatg tagagtctat aaaagatatt
    11161 ttgttataaa tatcttttat agacaaaaat attacaaaat ggtgtcgcat atttaaaaat
    11221 atgatattat agttaaggaa gtcatttaaa aaagaggagg atgcacttaa aatgaaatct
    11281 attttaatta tgggtggaag cgacttcata ggttctgcac ttgctaagcg cttgattaaa
    11341 tgtgggtatc aaattgatat acttacaaat ggtaaaaagg aaatagatta taatggtttt
    11401 aaagaacatc taatttgtga tagaaaagtt cgaaaagata tggaaaacat tatcacagga
    11461 agaaaatatg attacatata tgatatgaca gcatatacaa aagaagatgt aagtaatctg
    11521 attgatttta taagtatgga taacttaaaa aagtatatag tattgtcagc aggagcagta
    11581 tataaagata gtggaagaaa tataaaagaa gaaaatgaga aaggtgaaaa tgaaaactgg
    11641 ggaaaatatg ggttaaataa aaaagaggct gaagatttta ttataaatag ccctatacca
    11701 tatataatta ttagaccaac ttatatatat ggtgaaaata ataatttata tagagagtat
    11761 tatttctttg aaaaaataga aaaaaatgaa aagataccag taccaaaagg aaaacaagtt
    11821 agtaatcaat ttatatatat tggggatttg gtaaaggtat tggaaagcat aatgaaaaat
    11881 cctcatgtaa gagaggccta taatgtgaca aatccacagt taatttcttg ggatgacctt
    11941 atatatacat gtggagaaat tattggcaaa gaacctataa taaaatatgt agatatggaa
    12001 aaagtagagt ttagagaaag aacgtatttc ccatttagaa atatagactt taatcttgat
    12061 ataaataagc ttattgaaca tgggctttat ataccaaatg tgttactaaa agaaggtctt
    12121 acagcaacat ataaatggtt ttcagcaaat aagccaaaaa tgcatgacag aaaaatgaac
    12181 aaagttgagt ctgtaatgca aatagtttaa aaaaataaat aagtaggtag atattttgaa
    12241 ctcaaaaatt aagtattttt taaaattttt tatcttaata tttattttga tttttatata
    12301 ctcagttgcg atagagccga gtctattaat tgtgaaaaaa tataatataa gtgaaaatgg
    12361 aacattgata aaaagtgata caattgagag taaatctgaa aggataaaag tagttcaaat
    12421 ttcagatact caaataggta gtttttattc aactaagaat ctcaaaaaag tagctaataa
    12481 gattaacact ttgaatccag atataatagt atttactggt gatttaattg attactccaa
    12541 taaaaatcca agtgtagatg aaattacaac tattttatca agtatgaatg ctaggttggg
    12601 aaaattttca gtatttggca atcatgatta tatgtataaa cttcctaggt attataggca
    12661 gattatgaaa aattccaact tcaatctttt agtaaatgaa aataaaaaaa taaaattaaa
    12721 agatgataaa tatattaata tattaggtgt tgatgaaata ttaaatggaa atcctaatat
    12781 aaaatatctg gagagtcaaa tagataataa gaattttaat ttattgttag cacatgaacc
    12841 agatttagta gatatgctta gtaaagatac aatgaattta gttctatctg ggcatagtca
    12901 tggtggtcaa ataagattac ctattaaggg agcattagtt actcctccat atggaaggaa
    12961 atatactaaa gggttttatg atataaatgg aaatcattta tatgtgagtt cgggtcttgg
    13021 aagtacaaag ttaccattta gattttttaa tataccagag ataatagaat ttaatattat
    13081 aatctaacat aaaatattta gaaaatataa ttttgctttt tataatatgt agatgtatat
    13141 actgtaaaaa tggattaaac cagtagattg aatgaattaa tagattaaat taatatataa
    13201 aatatttaag ttataattaa tgtattagac aaaat
//
LOCUS       DACULQ010000113        13101 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.113, whole genome shotgun sequence.
ACCESSION   DACULQ010000113 DACULQ010000000
VERSION     DACULQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.113"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1723
                     /locus_tag="JA154_12740"
     CDS             <1..1723
                     /locus_tag="JA154_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HAT7996715.1"
                     /translation="VIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTN
                     DDIEVLLASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAI
                     IFSSEYKFLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGIL
                     SLKRYNKLEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGG
                     IDSSIITTVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIK
                     SLPNVIYSLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMK
                     SVVNSPTYIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFK
                     YKEKNIYEYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELR
                     VPFLYKDVFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWL
                     KDSLGDIVKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFI
                     EHKNVEY"
     gene            complement(1815..3251)
                     /locus_tag="JA154_12745"
     CDS             complement(1815..3251)
                     /locus_tag="JA154_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAT7996716.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKRSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            3580..4158
                     /locus_tag="JA154_12750"
     CDS             3580..4158
                     /locus_tag="JA154_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemical-damaging agent resistance protein C"
                     /protein_id="HAT7996717.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            4197..4775
                     /locus_tag="JA154_12755"
     CDS             4197..4775
                     /locus_tag="JA154_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemical-damaging agent resistance protein C"
                     /protein_id="HAT7996718.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            4861..5490
                     /locus_tag="JA154_12760"
     CDS             4861..5490
                     /locus_tag="JA154_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HAT7996719.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            5480..8239
                     /locus_tag="JA154_12765"
     CDS             5480..8239
                     /locus_tag="JA154_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HAT7996720.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            8326..9888
                     /locus_tag="JA154_12770"
     CDS             8326..9888
                     /locus_tag="JA154_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996721.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            9889..11025
                     /locus_tag="JA154_12775"
     CDS             9889..11025
                     /locus_tag="JA154_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAT7996722.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            11089..12165
                     /locus_tag="JA154_12780"
     CDS             11089..12165
                     /locus_tag="JA154_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT7996723.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNRHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            12143..>13101
                     /locus_tag="JA154_12785"
     CDS             12143..>13101
                     /locus_tag="JA154_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996724.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFA"
BASE COUNT         5173 a         1391 c         2352 g         4185 t
ORIGIN      
        1 tgtaattgat gctatttatg gaaataaacc atttgaaaaa gatggaaaag ttattgtttt
       61 taatggaaat atatataact atcatgaaat aaaagaagaa ttaattaaaa aaggacacaa
      121 ttttactaca aatgatgata ttgaagtctt attggcatct tatatagaat ttggtaagaa
      181 ttgtgtacat aagattaaag gaatgttcaa ctttataatc tatgatagag aaaatgaaag
      241 tatatttgga gcaagagatt tatttgggat aaaaccactc tattatataa ataaggaaaa
      301 tgcaataatt ttttcctcag agtataagtt tttacttgaa tatatgaaaa atttaaatat
      361 aaatgaaaga agcttacaaa gttatttttc atttcaatat gtacttcctg aagatactat
      421 gatacaagga ataaggctga taccagcagg tcactatttt agggtagaaa atggtatact
      481 atctttaaag agatataata aacttgagtt tagaagtagt acaaaatttt tttatactaa
      541 aaatcactta ggcaatagag atgtgaacga ggatgatata agaaatatag tagtagattc
      601 aatagtaact catatggaag aggagaaaga aattggaaca tttctgtctg gtggaataga
      661 ttcatctatt ataactactg tggcttcaca aattaatcca aatataaaat ctttttctgc
      721 aggctttagt gtcaaaggat atagtgaatt agaagtagca aagaaaacag ctgataagtt
      781 aggtatagaa aatatccaga ttaatataac acaagatgaa tatataaaat cattaccgaa
      841 tgtaatttat tctttggatg accctatagc agacccttca gaagtaggtc tttatttctt
      901 aattaaagag gcaggtaaac atgttaaagt agcattgtct ggagaaggag cagatgaatt
      961 atttggagga tataatatat ataaagagta tagtactatg aaatcagttg tcaattcacc
     1021 tacttatatt aaaagtatat taggaagagt ttcagaactt atgccaaaca taaaaggaag
     1081 aaattatctt tatagagcta caacaccatt agaaaaaaga tatataggaa atgcaaaagt
     1141 ttttgaaaac agcgaagtaa aaagattctt ttttaagtat aaggaaaaga atatatatga
     1201 atacctgttg tctaatttgt atagagatgc acaaaaaaat aattacgatt atataagtaa
     1261 aatgcaacat atagatgtga atacatggct tcaaggcgat atacttcaaa aaattagcaa
     1321 actttctaca gctgagcagg ttgaacttag agtaccattt ttatataaag atgtatttga
     1381 tgtagcaaaa aatttaagaa tggagcaaaa aataaataag aataatacaa aagttttact
     1441 tagagaggcg tttagagaaa tagtcccaga acatgttgtt caaaggaaaa aattaggatt
     1501 tccaactcca ataagagttt ggttaaaaga ttctctaggg gatattgtaa aggaaactat
     1561 ttataattca aatgtagatg aatttataga taaaaaatat gcaataaaac ttttagatac
     1621 gcatcttaaa ggtcatagag ataattcgag aaaaatatgg actatattta cattttgtct
     1681 atggcatcaa ttatttattg agcataaaaa tgtcgagtat taatactttt ttcaatatta
     1741 atcagagggc aaaagcatgc aatgttttca ccctcctgat tattatggaa taggataagt
     1801 agaattttga ttgattagtt tactatctca ataaaattat tggcaaatat tttgacaaga
     1861 ttactttcaa cagctaaatt taagttttgt ccatcatcat atccagcgga aatcaatcct
     1921 agtaagtttc caaacatatc taaaagagct cctcctgaac taccactaga gattggagct
     1981 gtaaattgta tcatttgaac cgtttcaaaa tcacgaaaag ctgaaataat tccatctgaa
     2041 actgtattaa aaagacctag agggcttcca attgcaacta ttttttgacc tctcacaggt
     2101 tttttactga ctttaacagg tattggcttg cattttctat caacctttat cacagctaaa
     2161 tcataatctg aatggtattt tataatttgg taagatgtat aaactttatc atcattttca
     2221 aatctaacta aaaatgaata cccatcattg actacatgaa gattcgtaag tatataacct
     2281 tcattattta ttactactcc agaaccgctc ttaaacggtt tattttcgtt attgcacaca
     2341 acaatcataa caacagaagc tgataacttt gcaaggtctt cataagaaag ttctacttcc
     2401 tcatcttgta caattttttt agatgttaga tttgtgttat taagtgtatt tttagagtta
     2461 ggtttattcg tagtaggaga agtcttattg gtattttcac tgtaatttat atttgtaata
     2521 tgcctttcaa aaggtatttc taatatattt ggagtcttcc tagaaagttc aaatagagta
     2581 gaaaattttg aacattcagg atataaatca agaatatctt tatttggatt tatatataaa
     2641 atatcacacc caagttgtga taaaaaatat aagaaaaaaa gttcttgcct tttaatattt
     2701 ccaaagtata ctatctttgg tgaaatagta gtcttcacct tgttataaaa taatttaggt
     2761 agaaaatagt ctatccaaca caaaagttta actgcaaagt ttttgatgat agaggatgta
     2821 caatttggca tacttgtgga aaataaatct aaagtatttt taaatgcaat ctttttagtc
     2881 caatttaagg tgtcattttc tattttacat ggaaagtcga tattatataa atcccaactg
     2941 ttaaacttag aactattata aatattaaaa attttaagca tcttatctgt ttgttctttg
     3001 tcaaaattta tttttagatt gtttaatcta atatagggaa ttgtaggatt attaaaaaaa
     3061 taagtatcta tatttttaat tttttctatg tatttatcaa ttgaatcatc aacacctata
     3121 tatcttgtaa agtagacttc tttaatatat gaagaacgct tatttttata ggtagtatca
     3181 gatataaatt caattagagg ttcagtagac ttttttattg aaatctttaa tggtttaata
     3241 gtacaaatca tattttttct cctataataa gatgatataa tctattataa aacaacttaa
     3301 aacaatatag tacaataatt ttgtagtttc aaaataaaat atgtacttgt ttatataaat
     3361 gatatataat tacatattaa acatagcttt tatgcattta ataaagtatg taaaatctat
     3421 gtaaaaagta agaaatataa acattttttt aattatattt taaaaatatc aagcaagttt
     3481 tattcacaaa tgaataaaaa atgttataat atgattaaag aaaaagataa ataattagca
     3541 agatttgttt agctaaaatt aacaagggag cgataaatta tgggtataac attatcaaaa
     3601 ggccaaaagg ttgatttaac taaaggaaat ccaggtctta aaaatatttt agtaggttta
     3661 ggatgggata ctaacaagta tgatggtgga tttgactttg atttagatac agcagcattt
     3721 ttaacaggag caagtggaag tgttactaat gatggagatt ttgtatttta caacaattta
     3781 aaacatacat ctggagcagt tgagcatcta ggagataaca gaactggcga aggtgatgga
     3841 gatgatgaac aaatagttgt tgatttatct aagattcctg gagaaataag caagatttct
     3901 tttactgtta caattcatga tgcaacagaa agaagacaaa attttggaca agtaagtaat
     3961 tcttatataa gaatagtaga taaagacaca aatgaagagt taataaagta tgaattagga
     4021 gaagatttta gtatagaaac agctatagta gtagctgaaa tatataagca caatggagaa
     4081 tggaagttta atgcactagg ttctggcttt gaaggtggtc ttgctgcatt atgtggaaac
     4141 tttggaataa acttataatt tcaactataa caataaacta aagggggata ttttccatgg
     4201 ctatagtatt aaaaaaagga caaaaaatcg atttaactaa gggaaatcca ggtcttaaaa
     4261 atataaaact tggactagga tgggatacaa attcatttga tagtggctat gattatgatt
     4321 tagatgtaag tgttttcatg gttggagaat ctcaaagagt tgaaaaagat gaagatttta
     4381 tattctacaa taacttaaaa catccatctg gagcagttga acacttaggt gataatagaa
     4441 ctggtgaagg tgatggagat gatgaggaaa tattagtgaa tttagagaca atgccaaagc
     4501 atatacaaaa gatagctgta acagttacaa tatatgaggc tgctgaaaga agacaaaatt
     4561 ttggacaagt aagcaactca tatataagag tattaaattc tgaaaatgat gaagaaatat
     4621 taagatatga cttaggtgaa gaatttagta tagaaactgc aattacagta tgtgagatat
     4681 ataagtataa tggagaatgg aagtttagtg cagttggagc aggatttgaa ggtggcttag
     4741 aagcactttg tagaaactat ggcttaaatg tttaatttaa gcaatataat atattaaata
     4801 taaataacct atacaaataa tgtataggtt atattatata aaattttgtg gaggtttagt
     4861 atgtcgatta atttatcaaa aggtgataaa atagatttaa aaaaatctaa cccaggactg
     4921 agtaacatac ttgtaggtct aggatgggac cctgtgcaac aatctggcgg aggttttttt
     4981 aaatcgttat ttggtggagg acaagctgat atagattgtg atgcatctgt atttatgctt
     5041 aatcaagaag gtaaattaag tggaataaaa gatttaattt attttggaaa tctaaaaagt
     5101 gcctgtaaat cagttttgca tacaggagac aatctaactg gtgagggtac aggtgatgat
     5161 gaacaaatcc tagttaattt ggataaagtt ccatcaaata ttcataaatt attgtttgta
     5221 gtaaatatat acaattgtgt agatagaaaa caacactttg gaatgataga aaatgcctat
     5281 ataagagttg aagaccaagg taataagaaa gagatagcaa aatacaatct atctgataat
     5341 tattctgaaa aaactactct tatagtagga gctatatata gaaaagatgg aagttggcaa
     5401 tttaaagcaa ttggtgaagg aacaaaagat gcgggtctta aagaagtaat gcaaaattta
     5461 gacaggattg agtgtgcata tggaatttaa taatgatatc aaatattata atgtagataa
     5521 agaagaagtt acaaaagact tatctgttaa tcctgaaaaa ggtttatcag aatcagaagt
     5581 caagactaga agagaaaaat atggtttgaa tgaatttact ccaaaagaag agggtagttt
     5641 ttgggatgat ttaaaggaaa gtttaagtga accaatgata gttatcttga ttattgcagc
     5701 agtagtaagt gcagtaattg gggaaactca tgatgcaata gggattgtag gtgctatagc
     5761 aataggtata gcaataggta tgattacaga aggtcgttct aagaaagcag cagaggctct
     5821 ttctaagttg actgaaaaca tagaagtaaa agtattgaga gatggggaag tacatcaaat
     5881 ttctaagagt gaattggtac caggtgatat agtatatatt gaaactggtg acatggtacc
     5941 tgctgatggt agacttatag aaagtataaa ccttaaaatt agagaagata tgttaacagg
     6001 agaatctgat gacgtatcta aaaagtgtga tgtagtagtt tctatggaag agatagaatc
     6061 taaaggagca gtagtagtac aggaaccaat acctgcaaaa cagataaata tggtgtttgg
     6121 tggtacactt gtagcttatg gtagaggagc tctagttgta acttcaattg gagataatag
     6181 tgaaatgggt aaaattgctc aaaacttatc agaaacagat gagcaaactc cacttcagat
     6241 aaaacttgga aatttaggtg gtttgatagc caaagtttct agtgcagtag caggattact
     6301 atttatattt atggttttcc aaatgataag taagaatgta ttaaatgtag atatgtctgg
     6361 aatattaccg tttttagaat caatagaccc agttaaaaca gcatttacag tatgtgttgc
     6421 gttgatagtt gcagcagtgc cagaaggtct tccaactatg ataaatatga cacttgcaat
     6481 aacaatgcaa aaaatggcta aaataaatgc attggttacc aaaaaagaag cttgtgaaac
     6541 gataggttct gtttctgtaa tatgttcaga taaaactggt acattaactg aaaatagaat
     6601 gactgtagaa gttgcatatg ttgatggcag atatattgaa agttcagaag aggaaataaa
     6661 tagttatttt gaagaaaact gtatgataaa ctctacagct gatgtagagc acaatgatgg
     6721 tgatattaag tatttaggaa gtgctactga atgtgcattg cttttatatt acaaaaacgt
     6781 agattataga caagttagaa aaaattctaa tatagtagct caaaatcctt ttacttcaga
     6841 ttcaaagaga atgacttctg ttataggtca agataatcat catgtactat tatcaaaagg
     6901 agcaccagaa gtcttacttg aattatgttc acatattcaa agaggaaaag acatagttcc
     6961 attaactgaa aatataaaac atgagatttt agaagaaata aagaaattgc aaataaaatc
     7021 aatgagaact ttagggtttg catataaaga aatctcacaa gctgaagaag aagcagcagt
     7081 aactgctgaa agtgatgcta tgaatgtaag tgcaatggaa aacaatttag tatttagtgg
     7141 ttttgttggt ataagagacc cacttagaaa agatgttata gagtctgtaa atacagcaaa
     7201 taaagctggt gtttctgtaa aaatgcttac aggagataat ataaatacgg caagagctat
     7261 aggtgaagag ttaggcctat taaaaaataa tatgagagtg gttgaagctt catatataga
     7321 tactttagat gatgaagaat taaaacaaga gatacaaagt atttcaatcg tggcaagaag
     7381 taaaccagat agtaaaatga gaatagtttc agctctgcaa aagagcggag atgttgttgc
     7441 agtaacaggt gatggtataa atgatgctcc agcactttca aaagcagatg ttggtatagc
     7501 aatgggtata tctggtacag aggtatctaa aaacgcagca gacataattt taactgatga
     7561 tagttttagt accattgtaa aaggtattaa atggggtaga ggaatttatg ataacttcca
     7621 aagattcgtt caattccaat taactgtaaa tgtaattgca tttttagtgg ctataatatc
     7681 tcaagttatg ggacaagaga tgccatttac tacaatacaa ttattatggg taaacatcat
     7741 tatggatgga ccaccagcac tagcattagg tttagagcca gttagggacc atgtgttaaa
     7801 cagaaaacct gttgatagaa aagccaatat aatttctaag tcaatggtgt atacaatagt
     7861 attaaatgca ttttatataa cagcaatact aatgttacaa tctacattta atttcttagg
     7921 agttgatgaa gcgcataagg gaacagtaat gtttgcgtta tttgctttta gtgcactgtt
     7981 taatgcattt aactgtagag agtttaatgc agatagtatc ttccctaatt ttacacataa
     8041 caaattagca ctacaaataa tagtgttgac aggaattgca caagtaatat ttactcagct
     8101 attccaagat ttctttaact ctgtagcaat ggattttatg acatgggtaa aagttttatg
     8161 tgtagcagca agtgtaattg tggttaatga agttgtaaaa tgtataataa gactggtaaa
     8221 accaaaaaag actgcttaaa acaaattttt tagaagaaaa cagactgact caaaatatga
     8281 attttgggtc agttctgaat aaacatttaa attgggagga tagttatggg tgactcaaag
     8341 gtatttgaaa aaatattctc cagccaatca gatagaggaa attacactcc ttcaaaggga
     8401 tatctaagtt attttatttc gtatattggt ttagaagatg aagttttgta caatctcgag
     8461 atatttaaaa caaaacaaaa tattgatagc aaaaaagata ttgctttatt tactgatgtt
     8521 atagcaaatc ctagtgattt tgatattata aattatttta aaagtggact tcaaaaatat
     8581 agaactagca tggaagatgt agatataaat attttgggat ttgaagagat tgattataaa
     8641 ataaagcaag caatggatag agtgttaaaa gaagaggaaa aagaatttac taatgataga
     8701 gttaaacaaa attttatagt gaaaataatg gcatggataa aaatttatat cggtgcttta
     8761 gatataaata aaaatgaagc tccaaaagta atattttatg gagatataaa aaaacatgaa
     8821 gtttatttac tcttaatatt atacttggca ggttttgatg ttttatatct taatccaaat
     8881 tcaaagagta atatagatat tttaaaatca gaaagataca atatagagtt tgaagaagcc
     8941 aacataatag aagaaaaaat ttcttttgaa gaaagggtta ttttagggga aaaaatagat
     9001 aaaagttctg taaaaaaagc ttttactgtt ggggcagaag catcaaagcg tataagtgag
     9061 gaattgttaa atgatgcagg atttataaaa ccttggcaac tccaagatag aaaaataaaa
     9121 aatctacttc taagttcaac tgttgatgaa atatcaatat actggaatca accacttaaa
     9181 ttaagaccag gatttaagtt taatgatgct attgtagaag ctccaaattt tctttctaag
     9241 attaatggaa tatataacga taagaatgaa tatattaagt ttttagattt attaagagat
     9301 tcggaaagtt caacatttat tgaatttaat ggagatgtag atagattttc aaaagctttt
     9361 acaagagaag cttttagttt atccttttta ttggattcaa aaggtgcaat agataaaaac
     9421 tctgttttaa ataataaaga ttattccata tcaacattag cattgaatca acaaattatg
     9481 attttagaga aagtggaaga gttgttagaa ggaagtatgt ttttgaatgg tttaagtgga
     9541 gaagacaaga taaaaggtct atttacagta cttcacatgg ataagaaatt tgttcatatg
     9601 atgaacaatt tcgattactc acttataaac ccaaaattga ttatatacat gtacaagagt
     9661 attgtatttg ataaagaaat agtttttctt atgcttttac ttagtaagat tggatttgat
     9721 attatcatat tatgtccagg aggagaaaac aatatagaga atgttataaa taaccaattg
     9781 attgatgtac acagattgga taaaatggta tatgacctta aattaaattc gcttgaaaat
     9841 gatatacctt tacttaaaaa aatttttgga aagaggagac gattttagat ggggataaat
     9901 ataaatagtt tttcaaaaga taagaactct gtagaggtta ctgagaattt gcctacagca
     9961 gttcaagaag aaaacttcga tataatggaa tatacaaata ataaaaaaaa tgaactgaga
    10021 aagtctaagg aagttgaggc attgacatct cttatagaag tagaaaatcc agatacgatt
    10081 ttacagtttg gtaggaaagc atctgaagga gtagcaagag tttctgatag ccttttaaat
    10141 acaataaaat taaatagaaa tgaagaaaat tctaaaatgc tggttcattt aactaaaata
    10201 atggacaagt ttgatttaga cgattttcaa gaaactaagg aaccaaattt cgttcaaaaa
    10261 cttttcaaaa aagctaataa tgccattgaa atgatgtttc aaaagtatga aacattggga
    10321 ggagaagttg agaaaattca aattgagctt gaacaatacg aaagagatat agcactttct
    10381 aataagcaaa taggtgctat gttaaatgaa aactttgagt tttacaatga acttcaaaaa
    10441 tatattgttg ctggagaaat ggcaatagaa gaaatggata atgaaatact acctttcttt
    10501 aaacaaaaat cagagactag tggagaccaa atggatgttg tcaattatca agaactatta
    10561 aaagtttatg atatgttaaa tcaaagagtc tatgatttaa ggatagcaga aaatatagct
    10621 atccaaacaa tacctatgct tagaggtatg caacataata attatggttt aattagaaaa
    10681 ataaatagtg catttgtcgt aactttacca gtatttaaac aatgtttatc tcaagctata
    10741 ttacttaaaa agcaagaatt acaagcaaaa tctctaaaag cacttgatga caaaactaat
    10801 gaattattgc ttagaaatgc acaaaatgta agtactcaaa gtgcacaaat agctagaatg
    10861 gctggaacaa gttctgttca aatagaaaca ttggaaaaaa tgtacaatac tattaaatct
    10921 ggtatagatg aaactatgag aatagaagaa aacaatagag ctttaattaa ggataataca
    10981 aagagattgg aagaattaaa tacgactata atttataata agtagtatta tattaagggc
    11041 taatttgtat aaagttggcc ctttctatgt atatgaggag gctagacatt gaaatatttc
    11101 gattatatct gcaaagatga tttggagaga atttttttga aggagccaga agattttagt
    11161 gctaagactg aaaaagatgt gttgaaatat gcactgggcg cttttttata cgttccagct
    11221 actcaatata atatgattta taagagtatt ataggggatg ttaagggagt tagaccatta
    11281 gctatttgtt tagaagatgc tgttggagta aatggagagt tagaggctat agaaaatctt
    11341 agattaattt taaaaaatat tagcaatgaa agtataacta ataaagatgg aataccacta
    11401 atttttgtaa gaataaaaga tgtggagcaa ctgttgagaa ttaaagagat tataattaaa
    11461 aatagacatt ctataacagg aatattaatt cctaaagcaa attcggagtt aatagaaaat
    11521 tgtatagaag ctttagattc tatgaatctt caagatatgt atgttattcc gataatagag
    11581 acaaaagaat ttatatataa tgaaaaaaaa gaacttagtt ttactaattt gtacaatgct
    11641 attttaagac ataagtcaag aatattaagc attagagtag gcataactga tatattaggg
    11701 atgtatggta taaggagaga taagaacttc tctatatata ataatttgat ttgttcttca
    11761 tttatattag atataatcac atatcttcaa agaccagagt tagatatacc tataagtggt
    11821 ggagtatcag aattttttga tatgacgaat aaggatataa gaaataagta tatagaagaa
    11881 atattattgg ataaatatca tggattaatt ggaaagaccg taattcatcc aatgcaaatt
    11941 caaattgtac aagcgctatc tacagtatca tatgaagatt ttactgatgc tttggatata
    12001 ctggatagta cagacagtaa gtatggtgtg agcaagggtg tattaggaga gagaatgaat
    12061 gaaacaaatc cacatttttt atgggctaaa aaaacattga ttctatctaa aatatatggt
    12121 gtattaaata aaggagtaga ctatgaagaa ttgcttaaat tttaattgtg agattgacat
    12181 tattgataat ccattgaatt tagaactaaa tgatttttta gacgtaggaa taagaaataa
    12241 tagcaaaaga cgttttttgt ttatctcaaa gaaactcggt aaacatttag catgtaagcc
    12301 aagtgagatg gataatttgg gaaaattaat ggctacaatt tataatgaaa aaaataagga
    12361 agaatcaaat ggtactgtta tagcttttgc tgaaactggt acagctgttg ggcatagttt
    12421 ttttgattac ttatgtggag attatgaatt tattcatact actagagagc agtttgacga
    12481 gttagaaaat ctggaatttt tagaggaaca ttcccatgca acagaccaga atttatactt
    12541 tggtatgctt aacaacttta aatttggaga tgagataata ttggttgatg atgagattac
    12601 cacagccaat acatgcatga atataattag gaaaattcaa agcatttatc ctaaaaagaa
    12661 atacactata tgctcaatac tcaattgggt aagtgatgaa aatttaagta aagttactcg
    12721 tttagaaaga gagctagatt gtaagataga ttttgtatac ttatttaaag gaaactttaa
    12781 attttatata gatgaagata ttatttcaaa ttacaacgat aattatagta ttactaaatt
    12841 agagactatt tcaagtgata aagtaaattt agtagtaaat catatttatg ttgatttaga
    12901 agattatatt ggaaataaga aatatgtaaa atatacagga agatttggaa ttaatagaag
    12961 agaacaattt agattaaaag aaatcgttaa gagagaaagt aaaaaaataa ctatggccag
    13021 taaagaatct ttaaaaaatg aaaaagaaaa gattctattt ttaggtactg aagaatttat
    13081 gtatatacct atgctgtttg c
//
LOCUS       DACULQ010000114        12976 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.114, whole genome shotgun sequence.
ACCESSION   DACULQ010000114 DACULQ010000000
VERSION     DACULQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.114"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            114..569
                     /locus_tag="JA154_12790"
     CDS             114..569
                     /locus_tag="JA154_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAT7996725.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            618..1202
                     /locus_tag="JA154_12795"
     CDS             618..1202
                     /locus_tag="JA154_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family protein"
                     /protein_id="HAT7996726.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1207..1530)
                     /locus_tag="JA154_12800"
     CDS             complement(1207..1530)
                     /locus_tag="JA154_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7996727.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1682..2200
                     /locus_tag="JA154_12805"
     CDS             1682..2200
                     /locus_tag="JA154_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HAT7996728.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2376..3044)
                     /locus_tag="JA154_12810"
     CDS             complement(2376..3044)
                     /locus_tag="JA154_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HAT7996729.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3159..4574
                     /locus_tag="JA154_12815"
     CDS             3159..4574
                     /locus_tag="JA154_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAT7996730.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4773..5255
                     /locus_tag="JA154_12820"
     CDS             4773..5255
                     /locus_tag="JA154_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7996731.1"
                     /translation="MKTNTDVIMEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFT
                     NVVGCTIHQYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQ
                     VYRNKDEFYPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA
                     "
     gene            5252..5836
                     /locus_tag="JA154_12825"
     CDS             5252..5836
                     /locus_tag="JA154_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HAT7996732.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6148..7224
                     /locus_tag="JA154_12830"
     CDS             6148..7224
                     /locus_tag="JA154_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAT7996733.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7303..8940
                     /locus_tag="JA154_12835"
     CDS             7303..8940
                     /locus_tag="JA154_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HAT7996734.1"
                     /translation="MSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHCCPPS
                     CVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLRGPTG
                     ATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGATGATGIGITGPTGPTG
                     ATGIGITGATGLIGPTGATGTPGATGPTGATGPTGVGVTGATGATGATGADGATGVTG
                     PTGATGATGANGLVGPTGATGAAGTPGATGPTGATGPTGVGITGATGATGATGPTGAD
                     GATGPTGATGNTGADGVAGPTGATGATGNTGADGATGPTGATGATGADGATGPTGATG
                     ATGADGATGPTGATGATGVTGATGPTGPTGATGATGATGASAIIPFASGIPLSLTTIA
                     GGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTITSISAYFSTT
                     AALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGIVTGLN
                     IAATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            9109..9870
                     /locus_tag="JA154_12840"
     CDS             9109..9870
                     /locus_tag="JA154_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HAT7996735.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            10179..10457
                     /locus_tag="JA154_12845"
     CDS             10179..10457
                     /locus_tag="JA154_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996736.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            complement(10568..10747)
                     /locus_tag="JA154_12850"
     CDS             complement(10568..10747)
                     /locus_tag="JA154_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996737.1"
                     /translation="MQFPFYKITKLIESYEKVEYNQVFFSKQDLLILINFNFKHIKSY
                     YFLLDTLQPALLATV"
     gene            10750..11325
                     /locus_tag="JA154_12855"
     CDS             10750..11325
                     /locus_tag="JA154_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996738.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(11431..12264)
                     /locus_tag="JA154_12860"
     CDS             complement(11431..12264)
                     /locus_tag="JA154_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HAT7996739.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(12332..12961)
                     /locus_tag="JA154_12865"
     CDS             complement(12332..12961)
                     /locus_tag="JA154_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996740.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         4885 a         1649 c         2260 g         4182 t
ORIGIN      
        1 ttttaaggaa aaataacata tttgtattaa attttattga aatgattaaa tttatttact
       61 taatgtaaat tattagctaa aattgtatta aataattaag gaggtgagta gctctggatg
      121 aaatagattt aaaaataatt aattctctaa aaaagaatag tagggcttca acctctgaaa
      181 taagcagaca aattaattta tcagtaccag cagtctcaga gcgtataagg aaaattgagc
      241 agtcaaattt aattcaaaag tacactattc aaattaatcg ggaaaaaaca aaatataagc
      301 ttttggcttt tatatttatc aatactgatt tatctaaaag tatgaaagaa tttaagaaaa
      361 ctatattgga atatgatact gtattagaat gtcatcatat ttcaggagag tatgattact
      421 taataaaagt attagcagaa gatacaagta atcttgaaga gtttatatca gtgactttga
      481 aggaaattgg ggggattcaa aaagctaata caaccattgt tttatctaca attaaagaag
      541 aatttaatac aataaaagat attttttaaa taatttacta gtaaagtgtt aattatattt
      601 taggaggaaa atagaatatg aaagttttag tttttttagc aaaaggtttt gaaactatgg
      661 aatttagtgt atttgtagat gttatgggat gggctcgtaa cgattatggg catgatattg
      721 atgtagtgac ttgtggattt aaaaaacaag ttatgagcac atttaatata caagtattag
      781 ttgataagac gatagaagaa gtatgtgttg atgattatga tgctctagct attcctggag
      841 gatttgaaga atttggattt tatgatgaag catatgattc atctttttta aatctaataa
      901 gggagtttaa ttcaaaagaa aaaataatcg cttctatttg tgtggcagca ttgccagttg
      961 gaaaaagtgg tgttttaaaa aatcgtaaag ctactacata tcatttgaaa aatggtaaaa
     1021 gacaaagaca gttaagtgag tttgatgtaa atgtagttaa tgaaccaatt gttgtagata
     1081 aaaatattat aacctcatat tgtccagaaa cagctccaca tgtagctttt aagcttttag
     1141 aaatgttgac atctaaagaa caaatggatg aagttaaatt agcaatggga tttaaactgt
     1201 aatggtttat agattttcta agtaatcaga accccagtca cacatggaat ctaaaattgg
     1261 aataatactc tttccaaaat cagatagcga gtattctact tttggaggaa caacaggata
     1321 aaccactctt ataattaaac catctgattc taattcacgt aattgttgag ttaacatctt
     1381 aggcgttgct ttaggaatta atttttttaa ttcattgaac ctcaatatag tgtctttaag
     1441 atgccaaagt ataagtgtct tatatttacc accaatcaat gcaagtgttg cttctattgg
     1501 gcaactgtaa ttatctaaac attttttcat ttttgggctc ctttatttat tttaaagtat
     1561 ctttttaggt actatattac aaaaaagtgc attattgtaa tatagtacca tagtgataaa
     1621 attatagcat aggttaaata aatcatcaac ttagcctaat aaaacaatag ggggtaaata
     1681 aatgaatttc gttgaacttg caaaaaaaag atattcttgc cgtaattatc aagatagaaa
     1741 ggttgaaaaa gaaaaacttg agaaggtttt agatgttgca agaatagctc caactggtgg
     1801 taatcgtcaa cctcaaagat tgattgttat tcaggaaaaa gaaggtataa ataaactttc
     1861 taaggctgcc aatatatatg atgcgccatt agcaattctt gtatgtggag ataaagacaa
     1921 agtttggaca agaccttttg atgggaaaca acttacagat attgacacaa gtatagttac
     1981 agaccatatg atgttacagg caactgaact tggattggca agtgtttggg tgtgctattt
     2041 taatccagat ataattagag aagagtttag tttgccagat aatctagaac caataaatat
     2101 attattgatg ggatatgaat ctaagatacc agagagccca gagagacatg aaaaaactcg
     2161 tgttccttta agtgaaattg tttcttatga aactctttaa ttataaaatt aagttattca
     2221 taggataaat gtaataaagt gtttgttata aacaaaattc aaaataaata atatctacat
     2281 ataattaatt ttagtgaagt atatctagtt taactagaat gtatacttca ctaatttatt
     2341 tattcaaaat atttatgagg attttaatat attaactaaa atatatcaat tgaattaact
     2401 tttaaatctg gaaaagcact tgaaatatag tgttttatat catttaaatg gtcttgaatt
     2461 tgcttttctg atattttatc aggtccatat acaaaataca taatattttt agtatctttt
     2521 gttatagctg ttcgattatc agggccatta agaatactag ataatatacc aatattatct
     2581 gataaaaata aatcatcttt tgttaaaata tgttctttac cacctatacc ataaaaatgt
     2641 tcacttccat ttgctaattc tattttcaaa ggtaattcca ttttatcaag gtcatgacct
     2701 gctgttagaa gaagattttt caattctgct aaaaacattg actcaattgt tacacctaca
     2761 tctgggatac cttttgattt taatagaata gattctaatt gaagcaatac agggtaagtt
     2821 ttcttgaatt ttttataata ctttatataa cttgaaacag gctctgtttt aattttttct
     2881 ttgcgattgt attctgggtg ttgttctata atatttttaa ttgtagagtt tttaagaact
     2941 aaaaaatttt ctttattagg ttttgaatgt gtaatattta taaccataac tccaaattta
     3001 atattgggat aaatttcttt aactttatgt gatacctcta acataatata gactcctttt
     3061 tattttatat tttatgatat aatcttagca aattaaaagt ggattttata gttccatttt
     3121 tatagtaatt ttatagaacc acttttggag gtattagaat gtggattacg attaatagaa
     3181 atagtaatat atcattatca agacaaattt attgtgagat aaaagatatg attctggatg
     3241 gaagcttaaa gtttgagcaa aaactgccat catctaggac tttatcaaaa gaactatctg
     3301 tatctcgtaa tacagtttta gatgcatata atcaattaat tgcagaagga tatttggaaa
     3361 caaggaatgg atatggaaca attgtagcta aaggaatatc agggcataaa gttgatttta
     3421 agttaaatga tacttttaga gatgaaaaag atttacatac taataaacaa aaaaattata
     3481 tagattttcg ttcaggagtt ccagaattaa aactatttcc acgcaaagaa ctttcaaagc
     3541 tatataaaca aatactgtat gatttatcag attcggattt cagatatgga agtacagctg
     3601 gtgtatgggt tttaagagtg gaaatatcta agtatttatt tcgtactaga ggaattagat
     3661 gtaatcccaa aaacattatg attgtttctg gttcaacaca aggtttatct ttaatttcac
     3721 agcttttata caaaagtaat aaaaaggttg ctgtagaaga ccctattcat aaaggcttat
     3781 taaaggttat ttcatctatt ggatattcag ttgttggaat taaagtagat gataagggaa
     3841 tgaataccaa cttaataaaa tcaataaagg ggatttcttt tgtttatact acaccttctc
     3901 atcaataccc attaggaggg attttaccta ttcaaagaag actagagctt attaaatgtg
     3961 caacagagaa tgattactat ataattgaag atgattatga tagtgaattt agatatgagg
     4021 gtcaaccaat tagttctctt tatgaactta gcccaaataa agtaatttac ataggttctt
     4081 ttagtaaaat attatctcca gctattcgtg ttggatttat gttattacca gacaatattt
     4141 tagaaagcta taaaacctta aaaatgtact ctgatgttca tacagaaaca atattgcaac
     4201 atgtattagc tgaatttatt ggaaatggag gtttagagaa gtacatattt agaatgaaga
     4261 agttgtacaa tagaaaacgt aagtacctca taaaagtttt aaacacggaa tttaaaaatg
     4321 aatttaaaat aaaaggtgaa gcagcagggc ttcatatagt tgctcatttc tataatgtta
     4381 aatttacgga aagtttaatt cataaaattt ttgaagcagg tataaaagtt tatcctatac
     4441 aacaatatgc aattgagaat atattagaat atgactatga aattatacta ggttatgctc
     4501 atcttagttt tgatgaaatt gaaaaaggtg taaaaatact cagcaaaata atacatatga
     4561 atcaaggtac atagataaaa catgtagcaa tctacatata atagatgtaa ttaagtttaa
     4621 actttaaaag aaatatatat taatattttt gtataaaaat acctgatatt tttttatcga
     4681 tactttattg ctatacacta aattaagata gcaatttata ttaagaaaaa actttttgca
     4741 taggttataa tacttaaaag gagaggatag agttgaaaac aaatacagat gttattatgg
     4801 agataattga ttatatagaa gaaaatctta cagaaaatct aaatttagat aaaatagctt
     4861 atattgctaa ttattcaaaa taccatttac acagaatgtt tacaaatgtt gtaggatgta
     4921 ctatccatca atacatacag aggaggagac ttactgaagc agctagacaa cttatattta
     4981 cagacaaata tattatagac atagctatta cagtaggata ttctacacat caatctttta
     5041 cactagcatt tagaaattta tataaagaat ctccacaagt atatagaaat aaagatgaat
     5101 tttatcctat tcaacagaga tttgttttaa atctaaatca tcaaattcaa agtgaaaaat
     5161 gtatttctta tgcaaatatg aattataaac aaagaagaga tgaaatcaat tcttatctta
     5221 actttaatat taattgtgag gtgaaagtag catgagttta gtgccacatg taattgaaca
     5281 aacaggtcaa ggggaaaggt cttatgatat atattcaaaa ttattaaaag atagaattat
     5341 atttatagga gaagaaatta atgatgctat tgcaagttta gtagtagctc aattactgtt
     5401 tttagaatca gaagacccag attcagatat aattatatat ataaatagcc ctggtggctc
     5461 tgctacatca ggatttgcta tatatgatac tatgaattat gtcagatgtg atgtatcgac
     5521 tatttgtatt ggtatggctg caagcatgag tgctttttta cttgcaggag gaacacatgg
     5581 aaaaagatgt gctttaccta attcagagat tatgattcat cagccaatgg gaggagctaa
     5641 aggtcaagca acagatgtaa aaatagctgt agataatata ttgaaaataa aagaacgatt
     5701 agataaaatt ctttctgaaa atacaggtaa atcaatagaa gatattagga gagatacaga
     5761 tagagataat tttatgactg cactagaggc taaagagtac gggctgattg attatataat
     5821 gaataggaat gaataaagta tcaaagtatc atattaaaat gtctgtttta caaatatata
     5881 tataatatta aataagagtg tctcaaaatg aactttttag tttatgggac actctttttt
     5941 gtatgaaatt aaatatggtt ttatcatttc aacaattatt accaaatttc aagtttaata
     6001 aattctacta taaaaaatac ataaaagata aggttcttta gtaactttct gtcgtcaaaa
     6061 gtttttataa atttaataac aaaacaagag aaaataccat atattactta tgtctgcaat
     6121 atatagaaga caaaggagtt gtacattatg attacaataa gtttgtgcat gattgttaag
     6181 aatgaggaag aagttatagg cagatgctta gaatgtgtaa aggatatagt agacgaaatt
     6241 ataattgtag atacaggttc tacagattcc acaaaagaaa tagtctcaaa atatacagac
     6301 atggtgtacg atttcgaatg gatagatgat ttctctgctg caagaaattt ttcattttct
     6361 aaagcttcta aagactatac tatgtggctt gatgcagatg acataatttt agaagctgat
     6421 agagaaaaat tattaaagat taaagaaagc ttggatactt ccattgatat tgttatggca
     6481 aaatacaatg tatcctttga tgaaaatggt aacccaactc tctcgtatta tagagaacga
     6541 ttgtttaaaa gaagttctaa tcataaatgg gttgacccta ttcatgaagt agtacctctt
     6601 tttggagagg tattttattc tgacattgca atatctcata aaaagcttca tagacaggac
     6661 cctctaagaa atcttagaat ttttgaaaaa atgatttcag aaggaaaaac attggagcca
     6721 agacaccaat tctattattc tagagagttg tactataatg ctaggtataa agaagcaata
     6781 gaaggtttta ctaagttttt agactcatca agaggatgga ttgaggattg tatcagtgct
     6841 tgcagagatt tagccacatg ttattactta attaatgatg aaaaaagtgc cttatattca
     6901 ttgtttagaa gtttcgaatt tgatgagcca agagcagaaa tttgttgtga cataggaaag
     6961 catatgtttg atagacaaaa atataaagaa gccatttttt ggtataaggt agcattaaca
     7021 agagataaga atgatactaa tggaggtttt aagtcaaatg attgttatgg atatatacca
     7081 tctatacaat taagtgtctg ttatgacagg ttaggagaat cagataaagc tatttattat
     7141 catgaaaaaa caaaagaaat caaacctaac gatagtgctg ttttgcacaa tgaaaattat
     7201 tttagtaaat ttaaaaatag ttgataggtc acaatcatac taaatttgac atattatata
     7261 tagagcttaa aagctcaaat atatcaggag gtgcttttaa taatgagtag aaataaatat
     7321 tttggaccat ttgatgataa tgattacaac aatggctatg ataaatatga tgattgtaac
     7381 aatggtcgtg atgattataa tagctgtgat tgccatcatt gctgtccacc atcatgtgta
     7441 ggtccaacag gcccaatggg tccaagaggt agaaccggcc caacaggtcc aacgggtcca
     7501 acaggtccag gagtaggggc aacaggccca acaggaccaa ccggtccgac tggtccaaca
     7561 ggaaatacag ggaatacagg agcaacagga ttaagaggtc caacaggagc aacaggggca
     7621 acaggcccaa caggagcgac aggagctata gggtttggag taacaggtcc aacaggccca
     7681 acaggagcga caggagcaac aggagcagat ggagtaacag gcccaacagg agcaacagga
     7741 gcaacaggaa taggaataac aggcccaaca ggtccaacag gagcaacagg aatagggata
     7801 acaggggcaa cgggattaat aggtccaaca ggggcaacag gaacacctgg agcaacaggt
     7861 ccaacaggag caacaggccc aacaggagta ggagtaacag gagcaacagg agcaacaggg
     7921 gcaacaggag cagacggagc aacaggagta acaggtccaa caggagcaac aggggcaaca
     7981 ggagcaaatg gattagtagg cccaacagga gccacaggag cagcaggaac acctggagca
     8041 acaggtccaa caggagcaac aggcccaaca ggagtaggaa taacaggagc aacaggggca
     8101 acaggagcga caggtccaac aggagcagat ggagcaacag gtccaacagg agcaacagga
     8161 aatacaggag cagatggagt agcaggtcca acaggagcaa caggagcaac aggaaataca
     8221 ggagcagatg gagcaacagg tccaacagga gcaacaggag caacaggagc agatggagca
     8281 acaggtccaa caggagcaac aggagcaaca ggggcagatg gagcaacagg tccaacagga
     8341 gcaacagggg caacaggagt tacaggagca acaggcccaa caggcccaac aggagcaaca
     8401 ggagcaacag gagcaacagg tgctagtgca ataatacctt ttgcatcagg tataccacta
     8461 tcacttacaa ctatagctgg aggattagta ggtacacctg gatttgttgg atttggtagt
     8521 tcggctccag gattaagtat agttggtgga gtaatagacc ttacaaacgc agcaggaaca
     8581 ttgactaact ttgcattttc aatgccaaga gatggaacaa taacatctat ttcagcatac
     8641 ttcagtacaa cagcagcact ttcacttgtt ggttcaacaa ttacaattac agcaacactt
     8701 taccaatcta ctgcaccaaa taactcattt acagctgtac caggagcgac agttacacta
     8761 gctccaccac ttacaggtat attatcagtt ggttcaattt ctagtggaat tgtaacagga
     8821 ttaaatatag cagcaacagc acaaactcga ttcttactag tatttactgc aacagcttca
     8881 ggtctttcat tagttaatac tgtagcagga tatgcaagtg caggaattgc aataaattag
     8941 attagataag aaaaggcttc taatatcgta ttgatattag gagctttttt aagaatgatt
     9001 gatgaacata gttttaatat taattagtgc gcatattata aaatactatt atataatata
     9061 aaataataac attaaattgg aaatatagaa ttatctaggg gtgatactat gaaaaataag
     9121 attgaaatat caatgaacaa ctttgtaaaa aattatcata aatcaaataa tataactaca
     9181 aagtggcaag aacttttgtt tgcttatgca tctgctgaag atgatatgtt tcagaagcta
     9241 aaaagtgttg taggaacatc tcatgctatg ccaaaagatt ttctggaaaa tggaaaaact
     9301 ataattacat attttatacc ttttgatgaa agtgtaaata atagtaatat tggagggttt
     9361 gaatgttctg aaatatgggc aagagcgtac atagaaacta atatgttgat ttcaaattta
     9421 aatgatttct tatgtgaaga actagagaag ataggggcta attcatatgc tattcctggg
     9481 tcgtataatt ttgacagtga aaaattggta agtgactggt cacaaagaca tgtagcattc
     9541 atagcaggtc ttggaacttt tggtttaaat aatatgctta ttacagaaaa agggtgttgt
     9601 ggaagaatag gaagcataat cacaagttta gaattagagc caactaaaag accagaaaat
     9661 gaatactgct tgtataaaca taataatagt tgcaaaaaat gtgtcaataa atgcgtgaaa
     9721 gaagctttaa aagaagatgg atttaatagg aataagtgtt atgaaatgtg tatgataaat
     9781 gaaaaaaaat acacttatat aggtggatgt gatatttgtg gaaagtgtat agttggagtt
     9841 ccttgttcag taataaaccc atcaagataa tggatttaag ttgactaata attttaaaga
     9901 tagtttataa tgaaaaaatt ttagttatca aaataaatat aatataaaaa ctaaataaat
     9961 gtgtttaaaa aatggataaa taatttgtgt tgattgacaa ccagttatac tgttgataga
    10021 cttatatgta tcgctgtgat atataaggag ggttgagatt ttatgtttga tttaatcttt
    10081 atttttgcat agcaagggct attgcaacta tgaaatttaa tatataaata gccctttgct
    10141 attcctaatg aaaaattact atttaatagg agagcaaaat gagctataaa aaagcaatac
    10201 atatattacc agaagaatta cttgaattaa tacaggaata tgtagatggt gaatgtatct
    10261 atattcctag aaaatctaat aataaaaaag aatggggaag taatacacct actcgtgagg
    10321 agttgtcaat tagaaatatg cagatttacg aagactatca agttggatat gatttgcaat
    10381 atttatcgga aaagtattat ttatctttga aaagtatcca aagaattgta ttacaagaaa
    10441 aaagaaaaga tatctaagga tgagccagta tagttttata attatactgg ctcattttct
    10501 tttgataaaa gtgtttgagg tatagtttct tttaagtgga tgatataatt taagtaattt
    10561 caataactta tacagtagct aacaaagctg gttgtaaagt atctaataag aagtagtagc
    10621 tttttatatg tttgaaatta aaatttatta aaattaggag gtcttgtttt gaaaaaaata
    10681 cttgattata ttccaccttt tcataacttt ctattaattt agtaatctta tagaaaggaa
    10741 attgcattta tgaaaaaaat tacttgggag gtggaatata tctcccagtt acctgtaatt
    10801 aggtattgta aaaaatgtgg aaaaaagact gaatatattt gttcaggttt atttagggtt
    10861 aatgctcaac ataaatattt ggatatatgg ttaatataca agtgttcaaa ttgtgattca
    10921 acatggaact caactatata ttctcgtgtt aatccaaaaa gcttaaatcc agaaatacta
    10981 gaacaattcc atacaaatga ttataatctt gttaagcaat atgctatgaa tatggaactt
    11041 ttacatagaa atggagcaga agttggtatt ccagagtata aaatagtagg acaagaaatt
    11101 aatattaatg accttacaca agttcatatt attagtaaat atccatgtca gttaaaggta
    11161 tcaacattat tacgtgagaa actaggttta tctaggaagg tatttgacga aatgttggat
    11221 tgtgggatta ttagaagtaa ctcaggagtt gatttaaaga aaggtaaatt aaattatgaa
    11281 attatgttag atataggcaa ggagaagcaa aagtcttcaa attgattaga ataactaact
    11341 taatatattt tcaggaacat catttaaaaa aatagtgact ttaaaaaatt taaagccact
    11401 attttttaat atgttttatc tatataacta ttatttttct aggtttttta atatttcgta
    11461 atagtgtgtt gtggctattt cacttgactc gcctttttta caaaaagata atttagatag
    11521 ttttggaact acacgttcaa cgacaatttt attacctatc attttagatt caagtatctt
    11581 caatgcttct aaaaatctct tatccttttt tgcacgatta taaaatgata aaacgtagac
    11641 ataaaggaat aggttataat tacggaaagg atattcaact tgcataaata atgtgccaat
    11701 tccatagtga caagggccga ttggtttttt aatagtccaa tggtcaagta gaaaatctac
    11761 agctttgtca agttttgatt ctttatttag ataattacta aaacggaatg catttaatat
    11821 attaagagtt ggtagtggat ttgagtattc agtttctgga cctcgaccaa aactaaattt
    11881 attgcaacgc cacccaccat cagtatactg tgtatctaaa aaatgttgaa acgttttttg
    11941 cattctaatg tcagatgcat atcccatatg acagagtacg ttagcagcat ttgcagtatg
    12001 acaagggtaa atacctccat ttggatatag tttgaaacgt ccatcttctc gccatgtgct
    12061 aaatatcagg tcagcacatt ctttaagtaa agtatcatca gggttcacac ctaattctaa
    12121 tagataaagt acactatcaa gagtagagaa aggagaacct tttattaaac gcttatcagg
    12181 tgtagtccaa tagtctgcac cattatcata cctgtgtgat aatattattt caatatctga
    12241 taaagattga ttttctacat tcatttttta ctccatttca tattatattc tattaaatct
    12301 atcaaaaact ttattataat attatttata tttataattg atttatttta tttaaaattg
    12361 catttatagc tgtatcagga acaactgcta aatcttctgc attgaagtaa taaactatat
    12421 tagaatcaaa taatatattt gaatttggag gaaattcatc atctccaaac caaagtataa
    12481 ataccataga tgtattaccc atatatgtaa acctataagc taaatctccc atgtttactt
    12541 tttcagctcc aattttctcc ataactaact tatatttgtc tatttgagat gtaaaaactt
    12601 ctgaaagcct atatagagtc ctgtttaaaa aatttggata ataaacattg cctcctggta
    12661 tttctttaaa agttacatat ttattagttc taggaatacc tttagcattg attaaatatc
    12721 taactatcaa gattttaagc tcaaagttat tgcaaaggct accattacta ttccaaactt
    12781 ctccacttgg atagtctata atatattgtc tatcaaaaaa attgagtgtg aatctattta
    12841 aatcattatt gaaaggtata ttacttaaat tactaattgt atgagggtct aattctttga
    12901 gtttttttcg aacatagtca aatggaacat tataattatt ttttgttatc ttttcatcca
    12961 taattttttc ctccaa
//
LOCUS       DACULQ010000115        12914 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.115, whole genome shotgun sequence.
ACCESSION   DACULQ010000115 DACULQ010000000
VERSION     DACULQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.115"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            71..838
                     /locus_tag="JA154_12870"
     CDS             71..838
                     /locus_tag="JA154_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAT7996741.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            1121..1318
                     /locus_tag="JA154_12875"
     CDS             1121..1318
                     /locus_tag="JA154_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT7996742.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            1347..2423
                     /locus_tag="JA154_12880"
     CDS             1347..2423
                     /locus_tag="JA154_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HAT7996743.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            2426..3145
                     /locus_tag="JA154_12885"
     CDS             2426..3145
                     /locus_tag="JA154_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HAT7996744.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            3158..3697
                     /locus_tag="JA154_12890"
     CDS             3158..3697
                     /locus_tag="JA154_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT7996745.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            3854..4912
                     /locus_tag="JA154_12895"
     CDS             3854..4912
                     /locus_tag="JA154_12895"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088938.1"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HAT7996746.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            4930..5823
                     /locus_tag="JA154_12900"
     CDS             4930..5823
                     /locus_tag="JA154_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HAT7996747.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            5853..7061
                     /locus_tag="JA154_12905"
     CDS             5853..7061
                     /locus_tag="JA154_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAT7996748.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            7085..8344
                     /locus_tag="JA154_12910"
     CDS             7085..8344
                     /locus_tag="JA154_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7996749.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
     gene            8989..10452
                     /locus_tag="JA154_12915"
     CDS             8989..10452
                     /locus_tag="JA154_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter"
                     /protein_id="HAT7996750.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            10508..11632
                     /locus_tag="JA154_12920"
     CDS             10508..11632
                     /locus_tag="JA154_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HAT7996751.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKK"
     gene            11879..>12914
                     /locus_tag="JA154_12925"
     CDS             11879..>12914
                     /locus_tag="JA154_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyltransferase"
                     /protein_id="HAT7996752.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLK"
BASE COUNT         4966 a         1414 c         2542 g         3992 t
ORIGIN      
        1 taattttaat atataatatt tagtagagtg tgggagtagc acttaatatt tgcaggagga
       61 gtttttgtat atgggagaaa taataaatgc attagataga gccctagaca taattttgct
      121 gttatatcat gaaaagagag aaatgggaat aactgaaata agtaaggcta tgggagtata
      181 taaaagtact gtacatagaa ctttggttac tcttgaaaac aaaggttttg ttatacaaaa
      241 tgcagagaat ggtaagtatt ggctaggaat aaatttatat gcaataggta tggttgtggg
      301 agaaaaaatg tcattgactg aaatagtaaa accatatacg aaaaaattaa atcaagaatt
      361 taacgaagta gttaatgtat ctatattaga agaaagagct caagattctc caagaagtat
      421 cataatacat aaagagtatg gttcaaatca attactttca gtaaatccat ccgttggttc
      481 aagtagtgaa tgttattgtt cagcagttgg taaatgtctt atggcattta atgattctat
      541 agattttgaa aaatatagaa aaacaccaat acacaagtat acagaacata caattgataa
      601 ttgggatgat atgatgcttt ttttagcaaa aataaaagaa caaggatatg ctatagatga
      661 tgaagaacta gaacatggac taacttgtat tggagcacct atcttagata aaaataataa
      721 agctattgca gccataagtt tgtcaggtcc aactataaga atgagagaag gggactttga
      781 gtataagatt aaaagagtta tagaaactgc aaaaagtata tctgaacttt ttagatgatt
      841 tttaatctcg ataataaaaa taggctatat ttaaattgtt atttgtaaat atcagtttaa
      901 atgtagccta tttttcttta atctaaaatt aaatattctt aaaatttaaa atgttattga
      961 aatatataag cgaatgtgtt atcttttaat taagcaataa aaatagaaca aggttctata
     1021 ctatagaaca aaattgatgc atatatagta tttatacaaa atataaatat tatatattta
     1081 aaattaagta aaaatattac ataaagggga gatttgagaa atggaaaaag tcaagttgag
     1141 tgttgaaaga tgtaagggat gctatctttg tattgaagct tgcaaaaaag atgcaataag
     1201 tctttctgac tacacaaata aaaaaggctt tgtaactatt aaagtcgatg aagaaaaatg
     1261 tattcaatgt ggcgcttgtt atacaatgtg ccctgattta gtatttgaga tattataaat
     1321 tatttttatt agaggggaga aaaggtatgc aaagtttatt aattaaagga aatgaagctt
     1381 tagcagaagg tgctatacgt ggtggttgca gattttatgc aggttatcca ataacgccac
     1441 aatcagaaat attagaaatg atggcatgga gacaaaaaga agttggagga gtatttatcc
     1501 aaggtgaatc agaaatagcc agtgtaaata tggttatggg agcaagtgca cttggagcaa
     1561 gagctatgac ttcttcatct ggtccaggat tttcattaaa acaagaaggt attgcatatt
     1621 gggtatcagc tggtttgcca gcagttgtag catgtgtgca aagatatggt ataggtgatg
     1681 gatttatagg agctggtcaa gataattatt ggcaagcagt aaaaggcggt ggaaatggag
     1741 attatcattt aatcgtgtta gcacctaata gtgttcaaga aaatatggat atggcatatg
     1801 aatcttttga attggctgaa aaatatatgc atccagtact tatactttct gatgctacaa
     1861 taggtcaaat ggtagaacct tgtatagcac caccaatgaa agaatacgat atggataaag
     1921 ctccatgggc tgcaaaagga acacctgtag gagaaaaaaa taaagtaata actgatataa
     1981 catattttga agaccaagat acatggcaaa aagggtatat agaaaaaatg agaaaagttc
     2041 aagaagatga acaaagatgg gaagagatag aaatagaaga tgctgaagtg gtatttgtgg
     2101 catacggtat ttcatcaaga atagcagaag gtgcagttag agatgctaga gcaaaaggaa
     2161 tgaaattagg tctaataaga ccaaaaacat tgtggcctta cccaagaaaa gcatttaaga
     2221 aattaagtga ttcattgaag ggacttgtaa cagttgaaat gaacatgatg gcacagatga
     2281 aggaagatgt aattatagca tgtgataata gatttccagt atattcagta gcaacagctc
     2341 aatatatggc agaagtaaat caattaatag attttgctca acagattata gatggaaaag
     2401 ctaagcaaga ggaggtattt taattatggc taaaaaagca ccagcaataa tgatgtgtga
     2461 aaatatgttc tgtggaggct gtggacactc tatagtcgat agaattattg gagaagtatt
     2521 aacagaaatg gatatatgtc aagatacaat tatatgttca gatataggtt gtaatcatat
     2581 gtttcaattt agtatgaatg tagatgttgt cgttccacct catggtaaaa tgggagcagc
     2641 aatggcagct atgaaaagag ttagaccaga taaatatata tttacaatag ctggagatgg
     2701 tggagcttat gcaatagggc ttggagaaac tatgtcagct gctactagaa atgataatgt
     2761 aatatttctt gttatgaaca ataatatgtt tgcaatgaca ggagggcaaa tggctccaac
     2821 tactatagaa ggtcaatata cagcttctac tccagcgggt agaaattacg cagatcatgg
     2881 ttcaacattt gatgttgtaa aagttatggg aaatttagat atagcatatc tagcaagagg
     2941 aacaataact aaccctaaag aaataaatac aacaaaaaga tacatcaaaa aagctctaga
     3001 aaaacaaaaa gaaaagaaag gtttttgctt aatagaagtg cttgtaccat gtccaacaaa
     3061 ctggggatta aagccagtag atgctatgaa gagaattgaa aatgaacttg ttccaaagta
     3121 tccacttgga gaatttattg attagggagg aaataatatg ttgaatgaaa ttatatgtgc
     3181 tggttttgga ggacagggag ttcttacagc aggaaaaatt ataatgtatg ttgcatataa
     3241 ggcagaaaag aaaattacat ggttcccatc ttatggaaat gagatgaggg gaggaagtgc
     3301 aaattgcaat gttgttgtaa gtgatgaaaa aattgcaaat ccttgtgtaa aaaatccaga
     3361 tatactattg ggaatgaaca gcgtatcagt agataagtat gaaagtttaa tgaaaaaaaa
     3421 tggttatatg tttgtcaatg aatcactagt aagtaaggat agaaagtaca gagaagacat
     3481 taatgttata aaagtacctg ttactgaaat agcacaaaat ttaggcaatg aaaaagctgc
     3541 taacatttgc atgttaggtg ctatggtaaa gagtttggat ttatttacaa aagaagaatt
     3601 tgaaagtggt atgtgtgaat actttgaaaa tcaaggcaaa ggtaaattca attctaaaaa
     3661 tgtagaagca tttgaagctg gatacgacta cgaatgaata ttttaaatat ttaaaataat
     3721 tattgaaata cttattttta tatgttagta tttaattaat aaaatataga acgtcgttct
     3781 gaacaatagg acgaagtctt taggtcggaa tacaagaacg acgttctgta tagtagaact
     3841 agggggtgtt aaattgaaag ttttagtaat aaatcctggt ggtacatcca caaaaatttc
     3901 tgttttccaa gatgaaaatg aaattttgaa aaaaaatata acccatacaa gagaagattt
     3961 aaaaaatttt tctaaagtat ttgatgaata tgattataga aaacaactga tagtagatat
     4021 tttaagtagt gagaatcata gtataaattc ttttgacgca gtagttggtc gtggtggact
     4081 tatgaaggct ataaaaggtg gtacctatac tgtgagtgaa gaaatgattg aggatatgag
     4141 aaatgaaata aatggggaac atgcatcaaa tttaggtgca ttattagcta aaactatagc
     4201 tgatgaaata ggagtacaat catttgtagt agacccagtt tctgtagatg aatttgatga
     4261 tgtatcgagg attacaggaa tatcagacat agaaaaagca agttggttac atgcattaaa
     4321 ccataaagct gtttcaagaa aaattgcaca aaaactaggg ggaaaatacg aggattataa
     4381 ctttattgta gctcatttag gttcaggaat atcgattgta gcacataaac aaggcaaaat
     4441 gattgatgga agtggtggta ggtctgatgg tccattttct ccagaaagga gtggtggatt
     4501 acttgcatat cctcttatag aattgtgtta ctccggaaaa tatactcatg atgaaatggt
     4561 cgcaaaagta agttcgattg ggggaatgta cgattattta ggaacaaagg atatgataga
     4621 aattgaaaac agaatcaagg agggtgatga aaaggcaaaa cttataatgg atgcatttat
     4681 ttatcaagta gcaaaagaaa tttctatgta tggagcttca ctttcaggga atgtagatag
     4741 agttatcttg acaggaggga tatctcactc agaaatggta gtcaaaggag tagcagaaaa
     4801 agtttcatat ctagctccaa tagagatagt accaggagag atggaaatgg aggcacttgc
     4861 tctaggggca cttagagtac ttaatggaga agaaatagca aaaacttatt aatatttagg
     4921 agaaagagta tgttaaaaga ttttaataat ttattagaag aggctaaaaa gcttgaacct
     4981 gttattgtaa gtgttgctgt tgcagaagac aaagaagtat taaaggctgt gaaatcagca
     5041 acagatttaa acattataaa gccaattctt gtaggagaca aggataaaat agaaagtata
     5101 tcaagagaaa ttggattaaa aggtcatgaa ataataggtt gtcaaaatcc cgatgaatca
     5161 gttaaaaaag ctgtagagat agttaaaaat aaggaagcta aagttcttat gaagggatta
     5221 gtaaatacaa gtgtctatat gagagctata ttaaataaag aaacgggtct tagaacagga
     5281 agattattaa gtttgcttgc agtatatgaa ttacctcaat atcataaact tctttattgt
     5341 actgatagtg gaataaatgt atcgccaaac ttaaatcaaa aaaaagatat aatgacaaat
     5401 gcacttctta ctatgaaaag tataggcatg gaaaatccta aaactgcgat acttactgca
     5461 aatgaaatgg tagaccctaa gatacaatct acagtggatg ccaacctact tgtagagatg
     5521 gaaaaatcgg gagaaatacc aaagtgtata gtggaaggtc caatagcatt tgatgtagca
     5581 tttgatgcac atgcagcaga acataaaggt attaaaagta atattgctgg tgatgttgat
     5641 ttattagtat ttccaaatat agaagcaggg aatatattag gaaagtcatg gcttagattt
     5701 aataaagcta agtgggcagg tatagtttta ggagcaacaa atccgataat tttaggttca
     5761 cgttctgaca cagctgaaat aaaaataaat tcaatagcat tagcgtgcct agcatcacaa
     5821 taagaaatta ttattaagga ggaaaaagta ttatgaaata tgcaaaaaga atggatatgg
     5881 taaaagcttc agctataaga gattctcaaa agaaaatagc tcagaaggta gcaagtggtg
     5941 gaactgttat atcttttgct gcagggcttc ctgacccaaa tttatttcca atagaagaga
     6001 taagtgaaat aactcaaaaa gtacttaatg aaaggggaaa atttgcccta gcatatggac
     6061 ctactaaagg tgaagatgag ttgttagatc tattagtaaa aagaatgaaa gaagaagaaa
     6121 atattgatac aaaagcagaa aatattataa taactactgg gtcacaacaa gggatagcat
     6181 taagtgctat gattttatta gaaaagggag atgtagtagt tacagaaaat ccaagttact
     6241 taggtgcaat aaatgcattt agaccttatg aatgtgattt tttaggagta gatacagatg
     6301 aagaaggaat ggtaacagat gatttagaca aattattatc tgaaaattct aacataaagg
     6361 tgatatatgt aataccaaca tttcaaaacc ctacaggaaa aacatggtct ttacaaagaa
     6421 gaaaagattt tatggaagtc gtgaataaat atgatgtcat agtaatagag gataatcctt
     6481 atggagaaat aagatttaca ccagaacctc tacctacact taaatctctt gatactaaag
     6541 ataaagtatt gtaccttggg tcattttcaa aggtattatg tccaggattt agagttgctt
     6601 ggatgtgtgg taatgtagac atcatagata aggcagaact tttaaaacaa ggtatagatt
     6661 tacagtcaaa tcaattttcg caagtacaag taatagaatt tttaaagaaa tacaacttaa
     6721 atgaacatat agaaaaaata agagcagagt ataagaaaag atgtctgcta atgttagatg
     6781 ctatgaagaa acatttccca gaggaagcta aatatacaga accagatgga ggaatgttta
     6841 tatgggttga acttcctgaa agtataaatg tagatgattt attagataaa gccattgatg
     6901 ctggagttgc ttatgtttct ggagagtcct tctttgcaaa taatggtcca aaaaatacta
     6961 tgagacttaa ctatacaaca atgtcagaag aacaaatcgt taaaggtgtt gagatacttg
     7021 cagaagttat aaaaaaagaa ctcaattatg cttgtgaata aatataaaaa gtgagggttt
     7081 aaatatggga gaaaaagaaa acggtataaa gtttggaaag ttaatgtttc aacttgttat
     7141 tatagcaata tcttgggaat tagtttatat tataccattt atccaatata cattatatga
     7201 tccaattcta aaagcattag aatgtacaaa cactcaacta ggttttctac ttacaattta
     7261 tgggttaggt aatatatttg gagctcctat aggaggctgg ttagcagata gatttgatta
     7321 tagaaagata attttaggtt ctgtatttct taatggagta gtatcatttt tatttgcatt
     7381 taatatgaac tatccatttg cagttgctac ttgggtaggt tgtgcaatca catcattagt
     7441 tatgaactat ccttctatgg taaaaatact tagagttata ggaaaagata atcaaggtaa
     7501 ggtttatgga tttaatgaag ctatggttgg agtttctgga gtagtaatgg gagcaatatt
     7561 cttatatatt tatacatgct ttgcaactcc tacattgggt atgagatggg taatgatatc
     7621 tttaggaata ttatcaatag caatgtgtcc agtattatgg tttgtaatta aagatgtaga
     7681 tattaaagaa gaaagagaag aagcaggtga aaaaatgtct gctggtgatt ttataacagt
     7741 actaaaatca ccaaatactt ggctagtagg aataagtata ttctgtgtat attcattcac
     7801 agttacaatg tcttatttta caccatatat aacatctgta cttggaggtt ctgtagcatt
     7861 atctggtgca ttagcaataa taagacagca tggtctaaaa ttatttggag caccatttgg
     7921 aggatattgt gcagacaaat taaaatctcc tacaaaagta cttttaccaa tatatgtatt
     7981 tggtatagca gtaatagttt tattcctagt attacctgca tcaactccaa tgactatatt
     8041 catagcttta acatttgtag taggtatatt aggttacatg ggaaaaggta tatattatgc
     8101 agtacaagat gaagtaaaag ttccagttaa atattcagca actactatag gtatagctgc
     8161 agctttagga ttttcaccag atgtattcca atttgcatta ataggacact ggatagatac
     8221 ttatggcaat aaaggttaca catatacatt tatattccaa atagcaattc tagttattgg
     8281 aatactatct tgtttatata tattaaaggt taaaaagaga agagcaagta aattagaagc
     8341 ttaaatattc aatttggtaa ttaagtatat ataaatacaa tacttatttt gatgaatgtg
     8401 attgtataaa taacataaaa aatatcaata gattaagttt tataataatg tttagagaat
     8461 aaagtataaa aatcaaaatg ttatcaagtt ttctaggttt taacataaag ttacccctga
     8521 aggtgtaaaa ttgagtggca attattatct attttcttac ctacataaac ctatttaatt
     8581 taaattctaa gagattttaa aaatagatgg tagttgtact caattttaaa cctcataata
     8641 aatacgaaaa tttaaaatct aactagatac tatttaatta tatattaata aatatatatt
     8701 gtactattta tataataata atttcaggct tttcattata tgtattccaa tttatattgt
     8761 ttgattatta ttcaaacaca cgtcgttaaa aattttataa atttatgttt agatatgatt
     8821 aatactcttg attttagaaa taaatatttg tagattgtag attaaagata atataaataa
     8881 atagaaggta gattttttag atatattaat ataaagatta tataaaatat tgaaatatag
     8941 ctgtttaaat tattgtctat aaatataaaa tgaatggtgg tgagttaagt gaccgattta
     9001 gttaacacca ttaatggaat tgtttggtca cccgtattag ttgcaatgtg tttgtgtata
     9061 ggattatatt tttcattgaa attaaaattt tttcaaataa gagatgtaaa agaaatgttt
     9121 actctcttgc ttgaaggaga tagttctcat gttggtatat catcatttca aggttttgct
     9181 acatctatgg ctggaagaat agggactgga aatatagctg gtgtagctgt agcaatagca
     9241 atgggaggtc caggagcatt agtatggatg tgtataatgg ctacattagg ttcagcaact
     9301 gcatttgtag aatctactct agctcaaata tataaagatg agcatgatgg gcagtataga
     9361 ggaggacctc catattattt tgaaaaaggt cttgggtgga aaaagtgttc agttttattt
     9421 tcaatagttg caataataag ttatacattt tttatgcctg gtacacaatc aaacacttta
     9481 tatctagcaa tgaatcaagc atttggattt tcaaaaatga caatagcagt agcatcttct
     9541 attgcattag cgataataat ttttggagga attaaaagga ttggtttatt tgctgaaaaa
     9601 gtagtaccta taatgggtgg agcatacctt attatgacat tagtaataat tataacaaat
     9661 attagtttag ttccagaggc tattgtaaca atgtttaaat ctgcatttgg atttggttca
     9721 gtttttggag gaatgctagg ttcagcaata tcttggggag taaaaagagg tatatactca
     9781 aatgaagcag gagaaggttc tgggacttat ggtgcggcag cagcagaagt gagtcatcca
     9841 gccaaacaag gattagttca agcattttct gtttatgtag acacattatt aatatgtatg
     9901 gctacaggta ttatgattgt aataacagga atgtataatg ttacaggtgt ttgcacagca
     9961 ctgccaggtg tagaaccggg accacaatat gtacagagtg caataaatac attacatcca
    10021 caattaggtg atatttttat agcaatagtt ttattattat ttacatttac aactttatta
    10081 gccttctcat atatgatgga gactaatgta tcttacttag taactagaat taaagcagaa
    10141 aaacatgaaa agtacataat taatgtctgt agatttatgc ttatagcatg tgcagcaatg
    10201 tgttgtttta tagaaccact tacaagctgg gcattaggtg atatatgtat aggtgtatta
    10261 acatatataa atttaattgc catctttaca ctaaaaaaac cagctataaa ggcatattat
    10321 gattatataa gacagaagaa gatgggaata aaaaggagtg atagaacttt taaccctata
    10381 gagttagata taagaaatgc tgagttttgg gaaaatagat acttaaaaga ggataagcat
    10441 gagttaaatt aaagttataa taaaatataa aaattactaa ataaatttag tgggaggtaa
    10501 gattggtatg aaagaaagaa taatacaagt ggctgattca aagaaagaaa aaattctagg
    10561 gctatgtcaa tctttatatg atgaaccaga aattgctttg caagaatata aatctgctaa
    10621 aaaaatatca gaatttttaa gagaagaggg ttttgatgtt gaggaaaact tggcaggtat
    10681 ggctacagct tttaaagcaa ccaagaaaaa tggagatggt cctaaaatag catttatagc
    10741 agaatatgat gctcttccag gcaatggaca tgcatgtgga catcatttaa ttgcatctat
    10801 gggagttgga gctggaatag ctctttcaag tatactggat acttacaagg gagaagtaag
    10861 tataattgga acacctgctg aagaaactgg agatggaaag ccttacttga tagaacatgg
    10921 tgtatttgat ggctatgatg cagcaatgat gatacatcca aattcaaaaa cttgtgtaac
    10981 acctgaaatt atagcaatag gaggtctaga tttcatattt actggaaaag catctcatgc
    11041 aggagctaag ccatataacg gaataaatgc tttagatgca gtagtattac tatataataa
    11101 tataaatgca ctaagacaac aattagtaga tggaacgaga atacatggta ttatactaga
    11161 agcaggaact gctgcaaatg taatacctga tatgggaaaa gtaagattag agataagggc
    11221 aaaagaacaa aattactttg atgaagtagt agaaaaagtg aaaaattgtg ctagaggtgc
    11281 tgcaatagcc actggatgtg aacttgaatt ttatcacttt gaaccaactt gccaaggatt
    11341 aaatgaaaat aaagttttgg tagacatatt tacaaagatt atggaggaat ttggaatata
    11401 tgaagatgaa caagtaatgt taggttcaac agatatggga aatttaagtc aaataatgcc
    11461 atgtatacat cctttgatga agttttctga aaatggagaa gaactacata caaaagaatt
    11521 tttggaggct tctataaatt catatgcaaa agatagagta attgatggta taaaaatatt
    11581 agctttaaca ggatttaatt tatttgaaaa tccagagtta ttgaaaaaat gaaggaagag
    11641 tagaacttag actattttta attatactct ttaaaataag aagagtgtct caaaatgaac
    11701 tttttagttt atgagacact cttttttgta tgaaattaaa tatattttta ttattttaac
    11761 aatgaaaaag aggcttatct gtttttatat gacctttttt aacttgtagc acaaaatatc
    11821 atgaatgatt acaattcgaa tgataaaatc gattataaga tatataggag gggtacatat
    11881 ggaaacttta gaagtaagaa atattgaatt gagtggtaca aattatgaga taggatacag
    11941 actaggagaa ttggttgcta atatgccaga gataatagaa ggtcagatta ataaatctaa
    12001 tctggtaagc aaaaaagaag aaaaagagat gatagagttg tttgataaat attgtccagg
    12061 tcttaatgaa gaattgcaag gttttgctga tgcaattcaa gtgaattgta atcaaatttt
    12121 atattatact atgacttatc ttaaaccagg ttgtagtcaa gttgcattag caccagaatt
    12181 aactgaaaat gggcatgtac tatttgcacg taatttcgat ttctcacaca atatggagga
    12241 ttttgtttta tgtaaaacta aggtaaatgg aaaatatgca catataggta ctaccgttat
    12301 gcaatttgga agaggtgaag gtatgaatga atgtggctta ggtgtaagtc agtcatcatg
    12361 tggtattcct gtaggcaatt cagatggttt aagaaaacca gcaattgtag gtttacagtt
    12421 ttgggctgtt attagatatt tgttagaaaa ttgcaaagat gtagatgaag cattagaata
    12481 tttaaaagat atgccaattg catacaatat taatttattg cttgcagata agtctggaaa
    12541 cattgcactt gtggagactt tagatgggaa aaaagaagta aatataatca atagtttaga
    12601 aagtaaaaga gaatattttt tacactctac taatcataca catattgata aacttcataa
    12661 attagagcca caatcaatga aaaattctat tcatagatat aaattaatta aagaatatat
    12721 aaataagtct aaaaaaattg gagagaaaga gttaatgaat ttattgtctt caaaatatcc
    12781 aaatggctta tcttgtaatt actataatga tttttttgga actttaaaaa gtatagttat
    12841 ggatttaaat ataggaaagt ttaatatttt atggggtgga ctagagaata aatgggagag
    12901 ctactattta aaaa
//
LOCUS       DACULQ010000116        12523 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.117, whole genome shotgun sequence.
ACCESSION   DACULQ010000116 DACULQ010000000
VERSION     DACULQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.117"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            435..797
                     /locus_tag="JA154_12930"
     CDS             435..797
                     /locus_tag="JA154_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HAT7996753.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            862..1479
                     /locus_tag="JA154_12935"
     CDS             862..1479
                     /locus_tag="JA154_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HAT7996754.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            1476..1961
                     /locus_tag="JA154_12940"
     CDS             1476..1961
                     /locus_tag="JA154_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087011.1"
                     /note="catalyzes the conversion of glutamine residues to
                     glutamate on methyl-accepting chemotaxis receptors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HAT7996755.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            2004..2522
                     /locus_tag="JA154_12945"
     CDS             2004..2522
                     /locus_tag="JA154_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HAT7996756.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFRVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            2658..3617
                     /locus_tag="JA154_12950"
     CDS             2658..3617
                     /locus_tag="JA154_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase response regulator"
                     /protein_id="HAT7996757.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            3643..5346
                     /locus_tag="JA154_12955"
     CDS             3643..5346
                     /locus_tag="JA154_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAT7996758.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            5607..7709
                     /locus_tag="JA154_12960"
     CDS             5607..7709
                     /locus_tag="JA154_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAT7996759.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            7722..8162
                     /locus_tag="JA154_12965"
     CDS             7722..8162
                     /locus_tag="JA154_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAT7996760.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            8198..9001
                     /locus_tag="JA154_12970"
     CDS             8198..9001
                     /locus_tag="JA154_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheR"
                     /protein_id="HAT7996761.1"
                     /translation="MIKLTDEEFIVLVNYVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            9041..9637
                     /locus_tag="JA154_12975"
     CDS             9041..9637
                     /locus_tag="JA154_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HAT7996762.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            9743..10606
                     /locus_tag="JA154_12980"
     CDS             9743..10606
                     /locus_tag="JA154_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298/DUF4163 domain-containing protein"
                     /protein_id="HAT7996763.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            10673..11662
                     /locus_tag="JA154_12985"
     CDS             10673..11662
                     /locus_tag="JA154_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAT7996764.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYIEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAINNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            11754..12365
                     /locus_tag="JA154_12990"
     CDS             11754..12365
                     /locus_tag="JA154_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAT7996765.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         5077 a         1227 c         2330 g         3889 t
ORIGIN      
        1 aaatctaatt taatcgataa tacatataga gaaatataat taagtaaata aacaactatc
       61 tattagatat tgagaaagtc aaatcctata tgaatctaca gaaatgattt taatgaatct
      121 gaaaaatttt tttatacaaa cataaaaaag gtataaagtt gttttattat aatttaatta
      181 cttttgataa ctttttatgt tttatacaat tatattttta ataactcgtc aaaacgccaa
      241 acatatatta caatagatat ataagttttt ttgttgacaa attatgttaa gagaattaag
      301 tataatatgg cataaataat tacttttatt tatcaatgaa atgtatttat tgtgtagcga
      361 aaaatagtca gatagtatag acaataactg aagataaaag cgtttattgt tgtacatatg
      421 gaggagttag ttttatggaa aataaaattt tgatagtaga tgatgctatt tttatgagaa
      481 tgataattaa agaagctctg agtaaaagtg gctataataa tttaatagaa gcaaatgatg
      541 gagaagaagc gtgtaatata tttaaatatg aaaagcctga tttgatgttg cttgatatta
      601 caatgccaaa aaaagatggt ttagaggtgc taagagaaat aaaaaagttg gattcaagtg
      661 ctaaggttgt aatgtgctct gcaataggac aagagaatat gattgtagaa gccataaaac
      721 ttggagcgtt agattttata gtgaagccct tcaaaacaga aacattagtt aaagttgtag
      781 ataatgcttt aaaataaaag tgtaaatata cactaataca atgaaaagca aataaatgca
      841 ataataaagg gaggggttta tattataaat tatttagaac ttgatgatgt tcatatagat
      901 gctcttagag aaattggtaa tataggctct gggaatgcaa ttactgcact tgcatccatg
      961 ataaatagca atgtagaagt attaatacct atggtcaaga tacttgaata taatgaagcg
     1021 acaaatttgc ttggtggtcc agaaaacaaa gtagtatgta ttttactcga tatgaaaggt
     1081 gatatcaatg gaatgtttat gtttttgttg gatgaatcaa taactcaact tatgctaagt
     1141 tctcttttta ataaagaaga agctttttta gatgagattg aggcaattga aatttcagca
     1201 attaaggaaa taggaaatat tatggcaagt tcatatgtaa atgctattgc atctatgtta
     1261 aacatgacta taagtgtttc aattccagat atatgtattg atatggttgg agctgttcta
     1321 aatgtaccta tgataagatt ttcagatgtt ggtgacaaag tcttatttat agaaaataaa
     1381 tttaaaatga gtgataatta ttttactagt cacattttga tgattccaga aatgagctca
     1441 cttagagaaa tactcgtgag gttgggatta gaagtatgaa tagagagata gtagtgaaca
     1501 ttgcagatat gaagatagcc tatagaccaa atgttttagt tacttatgca cttggttctt
     1561 gtgtaggagt atgtttgata gataaagtag ctggaattgg tggaatgtta cacgttatgt
     1621 tgccatatag taaagatgct attaacattg aaaataagtg taaatttgct gacacaggaa
     1681 taaatgagct cattaaatct atggagaata taggtgcaaa tagaatatat ataaaagcaa
     1741 aaattgctgg aggtgctcag atgtttttag gaagtagcaa ttcagtaatt gcaagtatag
     1801 gacataggaa tgttgaggca gtaaaaaaaa ctcttttagg attaaatatt ccaatattag
     1861 ctgaggacac aggtatgaat tatggtagaa caatacgttt ttacactgag acgggtgaat
     1921 tacttgtaga ttcaataaat aaaggtaaga agaaattata atatatattt catagaaagt
     1981 gtattttagg agggaggtat taaatgagat ttccagatga cccatattta gattatggac
     2041 taattgatga aggaattaag tatctaaagt ttagagtaga tgagcaagac tttggggttg
     2101 aacttgaaaa ggtaatagaa attactggaa atcaagaaat tatatttatt ccagaactac
     2161 ctacatattc taaaggcata ataaatctaa gaggaaaaat cattccgata atagacatgc
     2221 gtttgagact taaaaaagat gaattaatga ctacaaattg catgtatata gtagtaactg
     2281 aaataaagga cttggtagtt ggatttgtgg ttgatagagt tgatgatata gtaagcttag
     2341 aaaaaagtaa aatatcacct ccaccaagag ttactataga atattcggat tattttgtaa
     2401 gtggagttgg agaattagaa ggtaaaataa ttactttact agatttagag aaacttttga
     2461 cagataaaga tgaagtttta atagatgaat taatcaatgg gtttgattgc actatcatgt
     2521 aacataggga tgtagttgga gtgttattat aaaaagattg tgatgagaac atttaagaat
     2581 atagtggtat aattattaag tataaaaatt ttaaatcatt aaaatttgaa tatattatgt
     2641 ttaagagagg tttgtatatg aaaaataagc aacagggaac aattttagtc gttgatgata
     2701 gtgttctgat gtgtgactta atagataaaa cacttacaaa agataatttt aaggttaaaa
     2761 aagcatttaa tgcaatagag gcaaaagaat ttattgaaga atttatacca gacattattt
     2821 tgcttgatat aattttacca gataactctg gttttgagtt ttgtaaggaa gtaaaatcta
     2881 atgctcgtac agcagatata cctataattt ttataacttc aaaaaataca gatacagaca
     2941 tagtaaaagg atttggtgta ggagcaattg actatatggt gaaacctttt agtatgacag
     3001 aattaaaagc tcgtgttata gctcatcttg aagtaaagaa atctcaagat aaattaaaaa
     3061 agataaataa tgaactggaa tcttctcttg aaaaattgaa tagactcgtt gtaagagatt
     3121 atcttacagg tctttacaat aggcgacata taattaatca gcttatggag caaagaagaa
     3181 ttaataaata tagtgaaaca tcaatatccc ttatccttgg tgatattgat gactttaagg
     3241 ttatcaatga tacttatggg catgaagcag gggatttcgt gttaacagta atatcaagta
     3301 taataaaaaa gaattgtaga caaattgata ctgtatcaag atggggtgga gaagagtttt
     3361 tgataatgct cacaaatact ccaatagaag gtgctaagat actttcagaa agaatccgta
     3421 aagagataaa ggagtttgat ttcaactata aagaacataa aataaagtgc actattacat
     3481 taggagtagt agaagtaaac tcaaatatat cattagaaga aaacatatcc ttagcagata
     3541 aagcaatgta tgagggtaaa aacttaggaa aagattgtag cgtagtgagt acaatgaaag
     3601 gattaaaaaa gatataaact tactttatga aggagtacag atatgaaaag agaagtaaag
     3661 ctttctaaat tagcaattgt attgctgatt attatagtat tggctgttct agcagggatt
     3721 ttttcttcta tttcatctta taaagcattt gaaaaagtag aaaaaagtat taatgatatg
     3781 aataatttga ggactcaaag tcagtatata aaagatacaa ctagagagat aagtgatgta
     3841 ttaagaagat atgtatttac aggagaaaaa aagaataaag atatttatga aaaagaatta
     3901 tacggaaaaa caagagaatc agctttaaac aaattagaaa aaattggtct tacaaagagc
     3961 gagtcaaata aaatcttaga agcaaaagaa atatcagatt ctttagtacc aacagaacaa
     4021 agtgcaataa aggcagtcga aagtaatgat aagagtaaag ctcaaagtat aatttttggt
     4081 agtgactata taaactccca agagaaaatg gattctttaa taaatgaatt tcaagaggat
     4141 atagttaccc gttcaaattc caaaattgta gaggtaaaaa aagaattaca aagtctaata
     4201 ttaagaacaa tatttatact ttccctttta ataatattga caataataga atatacagta
     4261 ataaaattaa aaatagtgat tcctattaat aaagtagaaa aacacttttc aagaattgca
     4321 tctggggact taagaacaag tatagacgtt gaagaaagta aatctgaaat agggctatta
     4381 gtgagttctg tcaaaaagat gcaaagtatg cttttaaatt acatagacct tatagacagt
     4441 acattaacta gtattgcaaa aggagattta agagtagaga tagaccaaga atttataggt
     4501 gattttaaat caattaaaat atctttagaa agtataataa actcttttaa tggtgatttt
     4561 tctgaaataa atttatcagc agagcaagtt gcaagtgctt cagaacaagt tgcagcaggt
     4621 gcacaagctc tttcacaggg tgctactgaa caagctagtt cagtagaaga attagcttcc
     4681 acgattaatg agatatcaga tggaattaag aagtgtgcag atagtgctag tcttgcatgt
     4741 gaggtatctg accaatctgc atcacaagtt ggttttggaa gtagatgtat ggatgatatg
     4801 atgacatcaa tgggtgaaat aagtgaaaaa tcgagtgaaa tatcaaaaat aatcaaaaca
     4861 atagatgaga tagcattcca aactaatatt cttgcattaa atgcagctgt tgaagcagca
     4921 agagcaggtc aatatggtag aggatttgca gttgtagcag atgaagttag gtctttggct
     4981 ggtaaaagtg caaaagcagc tcaaaataca gctattctca tagaagaagc tattaaaaca
     5041 gtagaaaatg gtgtcagaat tgctaaagag actgcaaatg cattggactt ggtagtaaat
     5101 ggtgtagaaa aaactactaa ttatattaat ggtatttctt cggctgtaca tagtcaagaa
     5161 gtagcaatta cacagatttt atctggaata gagcaaattt ccatagtagt ccaaatgaac
     5221 tcagcaacag cagaggaaag tgcagcagcc tcagaagaga tgtctgctca agctcaaatt
     5281 ttaaaagaat tagtagaaaa atttcatcta aaggaaagtg aagtaagaga atcatcatgg
     5341 gtttaaatta tgaggtgaat attgaagtaa gagaattatc atgtatttaa attatgagat
     5401 gtatatatat tgaaataaga gaattatcat gtatttaaat tatgagatgt atatatattg
     5461 aagtaagaga ataattgtgt atttaaatta tgaggtacat atagtgagat aagagaatca
     5521 tcaaggattt aaattatgag atgcatatat aatagaaaat tatttgtagg tcgtaggtga
     5581 taaatgtgaa ttatattgaa ccgagtatga gacaaatgtt agatatgttt atatttgaga
     5641 caagtttatt actcgaacaa ttagatgaaa ttatgattca aatagaaaaa gagaaagtat
     5701 ttactcataa caacattaat gaaactttta gaataatgca tacgataaaa ggttcagcat
     5761 ctatgatggg gttagacaat atatctgctt tagcacatag tgtagaagac ttgttttatg
     5821 ctatacgtga aggtaaaata gaagttttag acaatgacct attatttgat ttagtctttc
     5881 aatcattaga ttttataaga agagatatag atgagttaca atcaagaggt tgtatatctt
     5941 ctgattactc taattttatt gaaggaattg aagagtatgt gagtactttg gaattatcaa
     6001 gttctatgaa atcaaaggat aatgcaaatt tagaaagtaa agacgaaaaa gaagtaatcg
     6061 attcaaattt tgaatacaaa gaagactctg atgtagtaaa aataatcttt gaacaaggtt
     6121 gcatgatgga aaatgtgaga gctttcatgc ttgtatttga acttagagat tactgtgaat
     6181 ttatagattt ttatccaaaa gatgtagaaa caaatgcaga gtctacagag tatattaaaa
     6241 gttatgggtt ttatatacaa tttgtagcta aagaggataa ggcaatagtt tataaaacta
     6301 tagaagataa tctctatgta aaagaataca taaatattag taaaaaagaa atagaaatga
     6361 aaaatacatc aaacaatgat gcagtagaaa ataaattaaa agataataat aaagtaagta
     6421 aaagttatca aaataactat acagaagatg agatacaaga agatgtttta aatgaaaaaa
     6481 ctgatatgga aaaagatagt ctaagtgaag aaaatgaaca tttaccaata agtaagcaga
     6541 gtattatgag tgtaaattta gaaaaattgg acttacttca aaatatagtt ggagaaattg
     6601 taattatgga atcaatggtt gtaaatagcg taaattctac aggtgttgat atagataatt
     6661 tcaataaggc aacaagacaa ttacacaagc ttacagatga attgcaagat atagtaatgt
     6721 ctatgcgtat gattcaaatt gctggtgtat ttcaaaaaat gcatagaatt gtacgagata
     6781 tgaataaaaa attagataaa aatgtagagt taataactat aggagcagag acagaggtag
     6841 ataagagtgt aattgataat ttagcagaac cttttatgca ccttataaga aatgctatgg
     6901 accatgcaat agagcctatt gaggagcgta tagcgaaagg taaagaagaa gtgggaaaga
     6961 tagttttatc tgctgaaaat ctaggcggag aagctgtgat aagtatttca gatgatggta
     7021 gaggcattat gagagataag attttggcaa aagctaagga acaaggtctt cttaaacatc
     7081 aaagtaatga gtatactgat gacgaggtca attctttgat tatgactcca ggcttttcta
     7141 caaaagaatc tataactgaa ttttctggaa gaggagtagg catggacatt gttcgtaaaa
     7201 gtattgaaaa agttggaggt tcaattgaag taaaaagtaa acaagatgaa gggacaacct
     7261 ttactataaa aataccatta actctttcta tagtagaggg tatgaagttt aaagttggag
     7321 attcactatt tactttacca atatcatcca taagaagaac atttaaactt acagacttaa
     7381 atcaaataat tacagaagga aataagacag agttaattac aatatatgga gcatgtattc
     7441 ccattataag gttacataaa atatataata ttcaaacaaa agtaaccaat ctgatgaatg
     7501 gtaatatgat aattattgaa aatgagcata aagcagtatg catttttgtt gatgaaataa
     7561 taggtaatca gcaagttgtt gtgaaaccat ttccagttta ttttaaccga tttaatataa
     7621 aaaataaggg tttatcggga tgtacaatac ttggggatgg aagtataagt ttaattatag
     7681 atgcagatag cattatagaa atatattagg ggatttaaag tatggctaaa gatgaaatta
     7741 ttaaacaagt attaacattt tatgtcaatg atgttattta tggaatagaa ttagaaaatg
     7801 tgattgaaac cataagattt caatcaataa cgtatgtacc atgtcttcca acatatataa
     7861 gtggagttat taatttgaga ggaagaataa taccagttat taatatgcat ataaagtaca
     7921 atcttcctaa agctgattat aatgagagaa cttgtataat aattacaaag gttgatgagt
     7981 atcaagttgg aattattgta gataaagttg ttgatgttat tcatatagaa aatctaaatt
     8041 tactagagac gactaattct aacaacacta atattaataa agatataaaa gagattgcga
     8101 aagttgaaga taattctata ttaattttag atattcgaaa gtttttgatt aatagcatat
     8161 agttagttct gataaattag caggaggaag tttttgcatg ataaaactca cagatgaaga
     8221 atttatagtt cttgtaaact atgtaaaaaa agaatatggt attgatttaa gtaaaaaaag
     8281 agcgttaatt gaaggtcgct tatacaatac aatgatagag aaaaaactta gttcttttag
     8341 tcaatatatg aatctattat ttaaagataa gacaggaaat gaagctataa acctaataaa
     8401 tagattgagt actaaccaca cattttttat gagagaacct caacattttg agtttataca
     8461 gaatagtata cttccttttt gggaagaaaa taataaaata agaaacataa atatatggag
     8521 tgcaggatgt tctagtggag aagaagcgta ttcaatagct atgactttgg atgattattt
     8581 tggatataat aaagagttgt ggaacattaa aatcatagcc acagatattt caactaactc
     8641 acttgaaaaa gctaaaagag gtatatacat agaatcaaat gttaacaatg ttcctgagtt
     8701 atggaagaag aagtatttta tagacaacaa ggatggtaca tttaaaattt gtgataagat
     8761 tagaaaaagt gtaattttta agccgtttaa tttaatgaat gatttctcat atcaacattt
     8821 tgatttgatt ttttgtagaa atgttatgat ttattttgat ttaaaaacaa gagaagaact
     8881 tataaataaa ttttataatg ctaccaaaaa agatagtttt ttgtttgtag gacatgcaga
     8941 agtaatcaac agaagtagta ctaaatacaa atatgtaaaa cctgctatat ataaacgata
     9001 aaaaggtatt tttgatatat gattttttgg aggcggtaat gtggaacgtt ttaataatat
     9061 ggttattgct ataggcgcat ctgtaggtgg tacagaagca atacttgaaa ttataaaaga
     9121 cctaccaaaa tcaacaccag ggattgttgt agttcaacat atgccagcta tatttactta
     9181 tatgtatgca caacggcttg acaagcagtg tattatgaat gtaagagaag ctaagaataa
     9241 tgatagagtc gaacaaggaa atgtgttaat tgcacctggg ggttaccaga tgaaattgtg
     9301 tacagataaa caaggatatt atgtaacttg tgaaaaggga gaaagaataa gtggtcactg
     9361 tccatcagta gacgttttgt ttgattcagt agcagaagta gcaggtaaaa attcaatagg
     9421 aataatactt acaggtatgg gttatgatgg agcaaatgga cttttaaaaa tgaaagaaaa
     9481 tggttcattt acaattggac aggatgaaaa ttcttgtata gtatatggaa tgccaatggt
     9541 agcttttgat agaggttctg ttatgttaca attacctctt ggtgaaattt caaactgttt
     9601 gataagacat attaatattt taaaacaaaa agattaattt tttataaata aaaattaatc
     9661 ttttattaaa ttatatggat aaaaaaccct agaaactcca aatataataa gagagattta
     9721 tatttaggag gaaaatgata atatgaaatt ttttaaagtc gatacaaaag ctaaagtttt
     9781 aagatatgta tttgttttaa cattgactat aatggctgtt ttggcaatta aacatgacta
     9841 taaatataat aatttaagga aagatgcttc aattaattta aatagtaata aggaagcagt
     9901 gactgttatg agtaaccagt cattaccaaa gggtaataca aatagtaaac aaactttatc
     9961 agtaagtaca ataggaagta aaacaatagt gaaaaatgat gagtatttag aaagtactat
    10021 aaacatgcct gtaataacaa attcaaataa aattgttgaa aggtctacaa atgataagat
    10081 aaaaaatgac atatttgaat tttataataa atcatataaa gaagcaaagc agtatcttaa
    10141 ggataatcct gacgagaaaa ataagtttgt tgcaaatgta gactttgaac ttaaaaagaa
    10201 tactgattct gctcttagta taaaagtgag atattatact tatagtggtg gagcacatgg
    10261 attttatcaa gatatagcat ataatgtgga tatgagaact ggaaaatttt tagaattaat
    10321 ggacttgttt aaggataata ctaaatataa agaagttata gatgaagaaa ttaaaagaca
    10381 aatagctgaa cttgaaaaaa aggatgaaga aaatatcgga atatataatt ttaaaggtat
    10441 aaaagagaat caaaactttt atttgcaaga tgagaatcta gttgtatatt ttgatttata
    10501 tgatataact ccatatgcag caggaatacc agaattttca ataaataaag cgttgataag
    10561 ttctatgcta aaaccagagt atgtagattt atttgatttg aaatagaaga ttaaattaaa
    10621 tgttatgcta taatatttag catagagact tattaaaatt gggggtataa aaatgctact
    10681 tttaaaaaaa gacgacataa aaaaagtatt tacaatgaga gatgcaattg aagctgataa
    10741 ggaagctttt agaatttatt gtgaaggaaa gagtgtaaat cctttgagaa ccaatatatc
    10801 tgttccaagt caagatgcta gtatgttatt tatgccagga tacatagaag aattaggctg
    10861 tgggggaata aaaattgtat ctgtatttcc taaaaatgct caaaaaggca aaccagtaat
    10921 tccagcaaca gtactattat tagatggaga aactggagaa gttagtgcag tattggatgg
    10981 cacttatgta actcagattc gtactggagc agcctctgga gcagctatag acgtattggc
    11041 taaaaaagat tctaagattg gagctttgat aggtctagga ggacaaggtg agccacaact
    11101 tgaagccatg atagaagcta gaaatttgga tgaggtaaga gtttttagta gaaacaagga
    11161 aagtagagaa aaatttgcag aagaaatgaa cttaaaatta agtaaatata atactaaaat
    11221 agttgcagtt gaatcatctg atgaagccat aaataacgca gatgtgatag ttttagctac
    11281 tccatcaaaa caaccagtac ttaatggaaa cttagtgaaa aaaggagctt taataagtgc
    11341 agtaggttca tatatgccag atatgcaaga gttgtgccca aactgtttaa ctagagcgtc
    11401 taagatattt tttgaatcaa cagatgcagt tttatctgag tcaggagata tattaattcc
    11461 attaaaagaa ggtaagataa gtaaagatga ttttagtggt gatttaggga atgttataaa
    11521 cggaactatt cctggaagag aagatgatga tgagataatt gtatttaaaa ctgttgggat
    11581 aggtgtacaa gatgtcgtaa cagcaaaaag aatatacgat aaagcaaaag aaaacggtat
    11641 tggaatagac tggaagtggt aaaatattca aaataattct atagttagac aaatttatgt
    11701 tataatgttg ataaataaat tttttattat tttaattgag ggaggatgtt tgtatgaaaa
    11761 tggtaaaaaa gttaatatta tctacgatta tggctctagt tttagttcta actatagggt
    11821 gttctaatgc agaagaaaaa gtaaagtcga attttgaagc taatttagct gcattagaaa
    11881 tgtataagta tgatgaaaaa ggaatttctg atgcattaac agaagctgaa tataaacaga
    11941 ttcaaactta taacagcaag gacaaggttt caatgattgc tcaattgaca cctctttatg
    12001 gtaaagaaaa tgctgaaaag atatacgaaa caattgttaa attaattcaa ggtgttgaaa
    12061 agacagtaac agtggaatct tctagtaagg ataagataac tttgaaagtt gtaagtaaag
    12121 ctttagatac tagtaaagta atgagtgaag cgaacacttt agtaaaatct tatgtaactg
    12181 atgaaaagaa ttacgataaa aaagaaaaag aagttcaaga tgatatgaca aaaatgatta
    12241 tagaccttta caataagaaa ccaactaagg aagttacaac tatgattaca tatactaaaa
    12301 aagatggtaa atggcaacca cctagtaata tacaaaagtt atgtgaagga atatatacaa
    12361 tttaatttat tttaaagaca caaaactatc ttttaagcat ataataaaat gtttggaaga
    12421 tagtttattt tattttcaaa gtataaatta tattttataa atgatttatt ttataaataa
    12481 tttatcttga taaatagtaa ataatttact ttactaataa taa
//
LOCUS       DACULQ010000117        12501 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.118, whole genome shotgun sequence.
ACCESSION   DACULQ010000117 DACULQ010000000
VERSION     DACULQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.118"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..871)
                     /locus_tag="JA154_12995"
     CDS             complement(<1..871)
                     /locus_tag="JA154_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAT7996766.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFAN"
     gene            complement(1064..1750)
                     /locus_tag="JA154_13000"
     CDS             complement(1064..1750)
                     /locus_tag="JA154_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAT7996767.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(1799..2350)
                     /locus_tag="JA154_13005"
     CDS             complement(1799..2350)
                     /locus_tag="JA154_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAT7996768.1"
                     /translation="MFKEKSMSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEH
                     EAVYTAEQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSR
                     LSFASPERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTV
                     NISRKGFEEFLEYLKYPIQSIDV"
     gene            complement(2376..3008)
                     /locus_tag="JA154_13010"
     CDS             complement(2376..3008)
                     /locus_tag="JA154_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HAT7996769.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(3098..4177)
                     /locus_tag="JA154_13015"
     CDS             complement(3098..4177)
                     /locus_tag="JA154_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996770.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(4310..5656)
                     /locus_tag="JA154_13020"
     CDS             complement(4310..5656)
                     /locus_tag="JA154_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996771.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(5725..7005)
                     /locus_tag="JA154_13025"
     CDS             complement(5725..7005)
                     /locus_tag="JA154_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996772.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(7132..7392)
                     /locus_tag="JA154_13030"
     CDS             complement(7132..7392)
                     /locus_tag="JA154_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAT7996773.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(7506..8441)
                     /locus_tag="JA154_13035"
     CDS             complement(7506..8441)
                     /locus_tag="JA154_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAT7996774.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEAKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(8479..10446)
                     /locus_tag="JA154_13040"
     CDS             complement(8479..10446)
                     /locus_tag="JA154_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HAT7996775.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYFVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNIDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(10457..>12501)
                     /locus_tag="JA154_13045"
     CDS             complement(10457..>12501)
                     /locus_tag="JA154_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAT7996776.1"
                     /translation="DMFTRTNLELTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVE
                     EPLINKSKIENRLDVIEEIKDDFILREDLNDILKNIYDIERICGKIAFERVTPKELIH
                     LKNSIEKLPNLKDTINLSNAKILKEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNII
                     KSDFSDELKELREISKNGAFLVKEIENREREKTGVKSLKIGFNKVFGYYIEITKANFK
                     QAKLDETYIRKQTLSNAERYITPELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNI
                     DRIQKVAKTIANIDVFVSLATVAHINNYVKPAINENNKLDIRNGRHPVVENIVGEENF
                     VPNDTYLNRGENIINIITGPNMSGKSTYMRQTAIIALMAHIGSFVPAESADIPILDRI
                     FTRVGASDDLSQGQSTFMVEMNEVSLILKNATERSLVILDEIGRGTSTYDGISLAWSI
                     VEYIQKNIRCKTLFATHYHELTDLEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADK
                     SYGIYVAKLAKLPDEVIERAKYILKDLEKNHVYNSVAINGDKENNNIINSNLDEELVK
                     VNQSNFKNKYESLKIEHELIVKDYKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKIN
                     QEDSVKEVALTQISFDSVNRDILSEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
BASE COUNT         3857 a         2187 c         1255 g         5202 t
ORIGIN      
        1 catttgcaaa agtatatatg gcattaactc ttgctacata taaattctca tcactacatc
       61 caatatatct agcatatttt tcaatctttt cttttgcatt ttcaacatca ttttcatcgc
      121 tctccattaa ctcatttata taaatatcca ttaatgacgc tttatattct cttatatcat
      181 agcctaaaaa ttcagtcaat ctctcaattt ccttactaaa atctccatct tcttttacca
      241 aaaattcgta tacatcttcc ctatatggat ttaaagttat tatttgtttt actactttgc
      301 attcgtcagc cttcccaata aaaccatctt tataattatt aaataaggca ttagattttt
      361 ttatagattc ttcatcaata taagaatgta ccgcagttat acgattttct ataagggcat
      421 ctacaacagc ataatgaatt ttaaaacaat cattgtataa ataattgctc attttcttta
      481 ttgtcgattc tatttctaag aagctcgcgt ggccattttt tttattttta tttggtataa
      541 taaaagtttt attcaaatta ttaaaaagtc tcttaaaatc taaatatttt tcacaataaa
      601 cacttttaaa ggtatcgata tctatagtta taattccaaa acttactata gtctcaattc
      661 ctttcttaaa agactcttct atatattttt ctcctaaact taaacctcta tcagatattt
      721 gctttagatt ttgaaaacta tttaaacaat ctctttcaaa gtttttagat atttctaaag
      781 catataattt aaagattttc cttatcttat tataattatt ttttgtatca tcaaatctga
      841 taatagctcc atttacatca aaagtaatca ttgagtttta acctccccat tagtttcatc
      901 aataaaataa atttaatata aactttataa tcataatatt aaacttattt atactataat
      961 tattaatttg atgataaaag ctattttcct tctaaataaa aaatttgtct aaagtttcta
     1021 aaaaatttag acaaatctta ggttattact ctttaaatta tttttaaatt attccaaata
     1081 cgccaaataa tattggtata aatatagctg gtaacaaatt ggctactttt atattcgtta
     1141 atttaagtac atttagacca agtcctataa ttaataaact tccagctgct gtcatttctg
     1201 tgactacagg atcacttaaa aatccattta aaaatgatgc tcccacacat ataatacctt
     1261 catacaaaaa tacagatatt gcagaaaaca ttacacctat acctaaagaa gcagtaaata
     1321 taattgaagc tattccatct ataacagatt ttacaaataa agtatcatga cttcctgtaa
     1381 gaccactttc taatgaacca actactgcca tggctcctat acagaataat aaactagatg
     1441 atataaaacc ctctgctata gaatcttttt tatttccttt agaaaattta ctttgtaaaa
     1501 aagcacccaa cttatttacc catttgtcaa tatctattaa ttctcctatc agagcaccta
     1561 cagctacgga tattattgtc actaatgtgt tttttccttc tagtgctcct gatataccaa
     1621 tgtacaatac acataaagca attccattca ttatagtatc gcttactctt tgaggtaatc
     1681 ctccttttat tataagacct actatacatc cacctgcaat tgctagacta tttgctatta
     1741 ctccaaacat tttttctccc ctcgattagc taattgaaat ttatcctatt attaaaacct
     1801 aaacatctat actttgtatt ggatatttta aatattccaa aaattcttca aaaccttttc
     1861 tagatatatt tacagtttta gtatttatgt taggatgaaa atttaagtaa tcttctttaa
     1921 ttacatcttt atctatgtaa agctcaactt ttctttctgt atcatttatt aatgaaaaag
     1981 gatttacact tccaggaaga agtttaagta cattgtatag tctttcaggt gaagcaaacg
     2041 ataatctaga acttcctatc ttatctttaa gtgagtttaa atcaacttgt ttttctcctt
     2101 taactacaat aagataatac ttatttcctt tggcatttct taaaaataaa tttttacatt
     2161 gacacccctt tgttattcca tctaaaatga ctaattgctc tgctgtatat acagcttcat
     2221 gctctactac atcatattca acatttaatt tatccaaaat atcatatatt ttagtttcat
     2281 tcatgctaga ttcattttca tcttgttctt ttttattatt taaatcactc atacttttct
     2341 ccttaaacac ttcatcataa tatcattgtg aggtattatt ctactatagc tattgctctt
     2401 actggagctc catcagcatc attatactta aatggtgctg ctataaatag aaatttttct
     2461 ggaacatttt ctaaatttgt aagattttct atgattattt ttcctttttc aaataaaata
     2521 tgatgcactt caaatactga tgtgtcatat ctatccactg atagcatatc tattcctatg
     2581 ccttttatat tagtattagc tatatagttt gcagcttctt ttgttaatgt aggataacct
     2641 acatagtact gctttttatc ccaaaatttt gaccaacctg atttaaatat tataaaatca
     2701 ctatttttta tttcatcttc atattttttt agatattcta attctatatt ctcatttccc
     2761 ttttcaagtt ccaatacata agcttttcca acaaaatttt ctatatccaa cttatctagg
     2821 gtctcacctt tagtatacat atgtcttggt gaatccatat gagttccatt gtgagaaaat
     2881 acactaatta atgtttcttg ataaccattt tcttcaataa tagctacttt cttgatatct
     2941 ggtctattag gctctgcata tacaggcatg tcattatgtg tcacatgagt taaatcaaaa
     3001 accttcatct ttatataccc cctatgtttt tattttctat tatataccta aattcatttt
     3061 atttctatca gattttattc cattatatac catactacta tatatttttt ataaacctca
     3121 agggatttac cgaaaaatct tggaaagaat tatcagtaaa tataacatct tcacccaaaa
     3181 aacgtttata ccttccttct tctttcttta aagtaattct ctgtttttgt gaatgattat
     3241 taaaaaccat tatcttaaga ggaggttttt ttctattagt ctgcactctt tttaacatgt
     3301 attttatgtg catatcagct tcaggaaatg aatatccaca aaatattaaa aggtcagtat
     3361 ctcttaaagt tttttctgtt tcattccaaa cattacttaa aaatatgttt gacatattct
     3421 taaaatatgt tggtggcact ataataggtt ctgttaactc accacactct aaacattttg
     3481 tctctgaact attttcaata agcttcatga caccaccttc atgtggtgta agagttacac
     3541 tattacaaat ggggcaataa agccaattca atgacccatg tatcttatat aatttgatta
     3601 atggttcttt tggttttctc caatttaact cctctttaaa atttgtaaac tccacaccat
     3661 aatcaagcat aattggcaaa tcattcttat ctcttaatcc agtaactgta ttgtctatat
     3721 gtatatcata atttgcacta ataaaagtag tatcaagaag caaatcattt tccaaaagat
     3781 tttcaactaa aagtttatga tatttattat ctgttctagg tgtattatga attgcttcag
     3841 ccattagcag tattaaatat tgtcttaaaa aacgtatact atcacttctg ttatttagat
     3901 tttctagtcc aaaatttcta aatgcttctt ttctttgttc tgctaagtct aaaagaccta
     3961 aaacttcttc aaaggtagga aaatttgcat tatctatatt atcttttact atatcaatat
     4021 tgaacatctg tttaaagaaa atatacaatt ctctattcat tttggtatta taatttgtgg
     4081 gatttagttt tttaaaatat tcgctaaaaa gttcattttg aataggtaga ttctctgctg
     4141 ctgaagctcc tgctcctaaa aatattgcta ttttcatata gttcctccta atataaatat
     4201 tactcctatt tttatatcca ttaaatatcc atatcattta ttttatgata caacaataaa
     4261 gctgtctttt actagaaaaa atcctattac aagacagcct tatattatat taaatcttta
     4321 ataattcgta aatagaaaga gcatatattt gagtagcttt tataatatcc tctactatta
     4381 agtattcatt agcttggtgt tctgtttctg gttttcctgg aaaaatagct ccaaatgcaa
     4441 cacaattatc taaagctctt gcgtatgttg ctcctccaga tgaaagtggt gttccatcaa
     4501 gaccagtttc ttcttcatat acttttctaa gtgtcttaac aagaagagta tcttctggaa
     4561 catagattga atccaagaaa tcatattctt cataatttaa attgtattta tctgtcattt
     4621 tctttaactc ttttacaaaa tcatcctttt tgtatgttac tggaatcctt acatctattc
     4681 ctgcaaattc tttttcgtta tcaatagtta ctctacctat atttactgtt agtttacctg
     4741 atacatcatc cttgcaatta ggaattatat tgtttccatt agcatcttca ccaataactt
     4801 ctgctaaaaa cttaattata ttagaatcta tacctatatt attaagagct atacaaagtc
     4861 tagccactgc atttatacca gtatcacttg ctgctgaatg gacacttttt ccaattatac
     4921 agattttatt accttcaaca gtatactcaa aatttaattt atcaagttct ttttttaatt
     4981 tatcagagta ttttccaata tagattgctt tacctggaac tgcatttaaa gcctttccaa
     5041 ctgacaactc tatatcactt ttgctatcac aagtcaaatg aacttgtaaa agacctttct
     5101 ctgcatttgt tattgggaat ctagaatctg gagtaaatcc atagtttggt atttcttcat
     5161 tattttcttt atatttattt atacatctcc aaagattttc ttcatcagcc ccaaaaatga
     5221 atcttatcct cttattaaag tctacattta aatccataag agctttcact gcataaattg
     5281 ctgatattgt aggtccttta tcatcttgtg ttcctcttcc atacaattta ccatcttcta
     5341 aaacagcttc aaatggaggg taattccagc tttctaaatc accttctgga accacatcaa
     5401 cgtgaccaag tatacctatc attttttcac cttgtcctat ttctgcatat ccataatatc
     5461 catctttgta tactgtctta aatcctaaag atttacataa gtctaatata cctcttaaag
     5521 ctttatctac attttcacca aatgggcaat tttcagttgc ttcagatata acactaggta
     5581 tttttacact ttcttgtatt gaagaaatca tttcatcttg tagagaattt actttttctt
     5641 taatttgctc tttcatatta tcacccttca attttcatca taatatacta ataaatattt
     5701 tgacctttta aaaaaaaaat atgcttattt tttcagtttt tttataaatt caatcgcttt
     5761 taaagtcccc atttttgaat ggtcaaatgt aagtccacct tgaaaataaa cattataagg
     5821 aggtcttatt ggagcatctg cacttaattc tatagaagaa ccttgtataa atgctcctgc
     5881 tgccattatg acttcacttt cgtatccagg catgtcccaa ggaacaggtt ttacataaga
     5941 atcgactggt gcagcttctt gtataccttc acaaaaactt attacttcat cagcattatt
     6001 aagtctaata cattgaataa tatcacttct taagtcatca tattttggta atacatcata
     6061 tcctaatttt tcaaaagctc ttgagcaaaa gatagctccc attacagcct gagatactat
     6121 ataaggcgcc aagaaaaaac cctgaagtac atttctagtt gttccaaaag taagaccaca
     6181 ttcttttcct attccaggag aagtcattct ataagatata agctcaatca aatcttttct
     6241 accagctata tatccacctg tcaatgcaag cccaccacca ggatttttta ttaaagagcc
     6301 agccataaca tctgctccaa catcagtagg ctctttggta tctaaaaact caccataaca
     6361 attatcaacc attactatag cttctggttt aacagacttt attacttcta tagcttcttt
     6421 tatatcacta attgaaagtg attttctcca agaatatcct tttgaccttt gtatcataac
     6481 gagtttagtt ctatcattta ttttgttttt aattccttct aaatctaagt ttccatcctc
     6541 taaaaaatct acatcatcat atgtaacacc atactctttc aatgaacctt tttcttctct
     6601 tatgcctata actccctcta aagtatcata tggtctacca gtaacagata aaatttcatc
     6661 tcctggtcta actatacctt gtatacataa agtaagtgca tgtgtaccat ttacaattat
     6721 tggtcttaca agagcatctt ctgtattaaa tactttagaa taaatttcct ctattttttc
     6781 acgaccaata tcattatatc catatcctgt agtccagtta aagtgcatat cacttagttt
     6841 agattcttgc atagctttta aaactttata ttgattatat tctcttattt cttttatttc
     6901 ttcaaactta tcttttattt cttccattat ttcttgcgat agtttaaaag tatcatcatc
     6961 tattccatag tagtctttta ataattcttt tgtctcatta agcattttat cacctgtctc
     7021 tttattattt tatttaaaca tgtaaaggag actattttac taaaacaaat aaattatagt
     7081 ttataagtta gtataagtct cctctcaatc atcttaaatt aaattaatta tctatctgtt
     7141 gttattatta ttgttgtttt ggttttgatt tgttagatta atatactttc caggttgtat
     7201 agttgataca gcatgtttat aaatcatgtt ttgttgtcta ttatcccctt ctattaatat
     7261 tatataacta tcaaacccct ttacaagccc tttaacttgc actccattaa ctaaatatat
     7321 agtaacaggt atcctttctt tccttgcatt atttaaaaac aaatcttgta aatttaaaac
     7381 tgtatttttc attagatttg taccctccca agattaaatt gaacttctaa aatattccta
     7441 ccttatattc tatataagtt atgacaaatt ttcaatatta agttgtatac ttaaaaattt
     7501 atatattatt ttatagaatc tttaatatat aagattattt cattttttaa ttcgtctata
     7561 ttttcatatt ggtctaaatc aaaccatttt gctgttttat atcttttaaa ccatgttatc
     7621 tgacgttttg cataccttct agaatctctt ttaattattt caatagcttc ttcgtatgta
     7681 tattctccat ccagatactt taatatctct ttataaccta taccttgcat tgatatcata
     7741 tcttttttaa atcccatact taaaagtttt tttgcctctt caaccaatcc atttttaatc
     7801 ataatatcaa ctctcatatt tattctttga tacaaatgtt ctctatctct atttagaact
     7861 attattattg gttgatattc ttcattaaat tttaaatcac ttgaaaaatc attcattttc
     7921 ttaccactta gacaaacttc aagagctctt atgacccttt tagtattgtt tttatggatt
     7981 ctattagcag attcttcatc aagttctttt aacttattat gcatgtaatc tattcccttt
     8041 tcagcaagct gtttttgaag ttcttctctt aattcattat tggcatcaga ttgtgcaaaa
     8101 tccatattat atattaaaga gtttaaataa agtcctgtgc ctccagttac taaaacacat
     8161 ttttcctttt catttatttc atgtatatat ttttttgctc tttgttgaaa ctctgaaact
     8221 gaaaatggat agtcaggctt aacttcatct ataaggtaat gcttcactcc ttgcatctct
     8281 tcctctgtaa cttttgcact tccaatatcc atatattcat atatttgcat agaatcagct
     8341 gaaattatct ctgcattcat atcttttgct aattttattg acaaatctgt ttttccaaca
     8401 gcagttggac ctgtaagtat tataagtggt atttttttca taacattcct ttccatatat
     8461 tttaaatatc caaatatttt acataattct tttaaacatt ttttctattt ctgtcttaga
     8521 tatttcgacc attataggtc ttccatgagg acatgtaaat ggattctcac acttttctag
     8581 ctgttccaat aaacttttta tttcaatatc gtatatttta tcatttgctt ttatagcaga
     8641 cctacatgcc atagatgcaa atctctctcc ttttaaatca taattacttg taatttcttc
     8701 tatattatca attatttgaa gtataaattt ctcagtttca ggtactccaa atatagtagg
     8761 aacacatctt accattatat gattattgcc aaaaatttct agctcaaaac caaatttcat
     8821 aaataactcc aagttatttt caatttgaag catatctata tttgatactt caataaccac
     8881 tgggtctaaa agtatttgca tatttatatc ttgtctatag aacttctcca tatatctttc
     8941 atatagtatt ctttcatgtg cagcatgttg gtccaacaaa tacatagaat cgtctttgct
     9001 caaaattata taagtattaa atacaactcc aattacaaaa tatccgtata aagaaaactt
     9061 gcgttttgaa ctacacaagt attcttcttc actgtcctta atactatctc ctaagtaata
     9121 atttccttca tttttaattc catcaacttg aaactcttct tgtatattat catttgcact
     9181 atcttcactt agataattaa tgttttttac atcatcttct acacttgcat ttaggacttc
     9241 acttactgat tgaaattcat ttattggttt ttcacttgat aaagtaacaa cttcaatgac
     9301 ttcatcctta cctttagtaa tattactatt tttaggagta gattctaata aatcattatt
     9361 tcttaattta taatctgtac ttttttctct actgttaata gcgattctag gttgtgtttt
     9421 tttatcacta taagtagcat attttccaat aagatttgag tgtattaatt taacctttaa
     9481 aaaatctcta agttctatat acacttcttg ttctttttca aatttaattt caagtttatt
     9541 ggggtgtata tttacatcta tacatgatgg gtctacttct atatttaaga agcacactgc
     9601 atgtttacct attggaatta tagacttata actttctgtt attgcatcta tgattatttt
     9661 actttttaca aatcttttat ttatatagat atgttgtaag tttttatttg aacggtatat
     9721 attgttatta cctatatatc cattcatttt aaagtgattg cacttaaact ctacatctat
     9781 tatattttcc gttatttctt ttccatatat acttctaata gtgtttacaa gttttccatc
     9841 ccctggagta tttagcatct gtttattgtt atttgtatat ttaaattgaa tgtttggatt
     9901 acctattgca agtttattta tcaaatcact tatgtttata gtttctgcat gagtagattt
     9961 taaaaatttc tgtcttgctg gtgtattaaa gaaaatatct tttataatga tggtagttcc
    10021 attagtagaa cctattggtt ctttcgatat tatttttcca ccttcaacat atattttagt
    10081 tcctattatt tcatctttag ttttagtcgt catttctaat tttgatacag ctgaaataga
    10141 tgccaatgct tctcctctaa aaccaagaga gtataaatca tacaaatcat caattttttt
    10201 aattttacta gtagcatgtc tcaaaaatga tttttcaact tcacttgaag gtattcctat
    10261 tccattatct gtaatcttaa ttagactttt tcctccatca attatatcta tggaaatttt
    10321 atttgcacct gcatcaatgg agttttcaat aagttctttt actacagaag aaggtctttc
    10381 tacaacttca cctgctgcta tcttatttat agtcaaatca tctaggatgt taattatatt
    10441 tttcattttt acacctctaa gattttattt cttttgcttt tctttgaaga ttataaagag
    10501 agtttatagc atctaatgga gtcatattta aaatatccag atttaaaatt tcttctgata
    10561 gtatatctct atttacacta tcaaaagaga tttgagttaa ggctacttct tttacagaat
    10621 cttcttgatt aattttctct ttatcatcat tttgcttcat taaagcaact tcatcattta
    10681 atgctttaaa tttagaattt aatttatcat aatccttttt tatatgtttg tagtctttta
    10741 ctataagttc atgttctatt tttagagatt catatttatt cttaaaatta ctttgattta
    10801 ccttaactaa ttcttcgtcc aaattactat taattatatt attgttttct ttatctccat
    10861 ttattgcaac actgttgtaa acatgatttt tttccaagtc tttaagtata tattttgctc
    10921 tttctatgac ttcgtctggt aatttagcta atttagcaac atatattcca taactcttgt
    10981 ctgctccctg aggaattatt tttcttaaaa atattattcc ttccccatct tctttgactg
    11041 ctattgaata atttttaacc tctttaaact cttcttctaa atcagtaagt tcatgataat
    11101 gtgttgcaaa taatgtctta catctaatat ttttttgaat atattccact attgaccatg
    11161 ctaaacttat accatcatag gtactagttc cacgacctat ttcatctaat ataactaaac
    11221 ttctctctgt tgcatttttt aatataaggg atacttcgtt catctctacc ataaaagtag
    11281 attgaccttg agataaatcg tcactagctc caactcttgt aaatattcta tctaatattg
    11341 gaatatcagc agactctgct ggtacaaatg agcctatatg tgccataaga gctattatag
    11401 cagtttgcct catatatgtt gatttacctg acatattagg accagttatt atgttaataa
    11461 tgttttctcc acgatttaaa taagtatcat ttggaacgaa attttcctct cctactatat
    11521 tttcgacaac aggatgtcta ccatttctta tatcaagttt attattttca tttattgctg
    11581 gttttacgta gttatttata tgtgcaactg ttgcaaggga cacaaaaaca tctatattag
    11641 ctatagtctt agccactttt tgtattctat ctatgttttt atatattgta tctcttatct
    11701 ctacaaatat ctcgtattct aaagatttaa tcttttcctc tgcatgtagt atcttttctt
    11761 ctatttcctt taattctgga gttatatatc tttctgcatt actaagtgtt tgctttctta
    11821 tatatgtctc atctagcttt gcctgtttaa agttggcttt tgttatttct atataatacc
    11881 caaaaacttt attaaatcct atttttaatg atttaactcc tgttttttct ctttccctgt
    11941 tttctatttc ctttacaaga aaggctccat tcttagaaat ctctcttagt tccttaagtt
    12001 catcactaaa atctgattta attatgtttc catcttttat ggttatcgta ggttcttcta
    12061 gaatagcttc atctattaaa ttgtatatat catctaattt atccatttct gaaacatact
    12121 cttttaggat ttttgcattt gataaattta tagtatcttt taaattaggt aacttttcta
    12181 tcgaattttt taagtgaatt aactcttttg gtgttactct ctcaaatgct atctttccac
    12241 atattctttc tatatcgtat atatttttaa gtatatcatt taggtcttct cttaatataa
    12301 agtcatcttt tatttcttct attacatcta atctattttc aattttagat ttatttatca
    12361 aaggttcttc tacatatttt cttaaaagtc tacctcccat tgctgtagaa gttttgtcaa
    12421 gtacatgtaa taatgaacct tttttcttat tccctcttat agtttgagta agttctaaat
    12481 tagttctagt aaacatatct a
//
LOCUS       DACULQ010000118        12412 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.119, whole genome shotgun sequence.
ACCESSION   DACULQ010000118 DACULQ010000000
VERSION     DACULQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.119"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1123)
                     /locus_tag="JA154_13050"
     CDS             complement(<1..1123)
                     /locus_tag="JA154_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996777.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFK"
     gene            complement(1136..1801)
                     /locus_tag="JA154_13055"
     CDS             complement(1136..1801)
                     /locus_tag="JA154_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996778.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(1883..2914)
                     /locus_tag="JA154_13060"
     CDS             complement(1883..2914)
                     /locus_tag="JA154_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996779.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITGDLKLIALLL
                     IYTMVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(2904..3590)
                     /locus_tag="JA154_13065"
     CDS             complement(2904..3590)
                     /locus_tag="JA154_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996780.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYKDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(3748..4785)
                     /locus_tag="JA154_13070"
     CDS             complement(3748..4785)
                     /locus_tag="JA154_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HAT7996781.1"
                     /translation="MFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRPNLCSEGTMT
                     YNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQYGASVVGIT
                     KLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICEVIETSKCYV
                     DASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAILTNKDYGSR
                     LRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGDDGKYNWVIE
                     HETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALDEYTSKFGKR
                     IFIPGNPSWLK"
     gene            5074..5745
                     /locus_tag="JA154_13075"
     CDS             5074..5745
                     /locus_tag="JA154_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996782.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            5747..6655
                     /locus_tag="JA154_13080"
     CDS             5747..6655
                     /locus_tag="JA154_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996783.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            6732..7418
                     /locus_tag="JA154_13085"
     CDS             6732..7418
                     /locus_tag="JA154_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996784.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGELL"
     gene            7415..9970
                     /locus_tag="JA154_13090"
     CDS             7415..9970
                     /locus_tag="JA154_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996785.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWSLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(10164..11363)
                     /locus_tag="JA154_13095"
     CDS             complement(10164..11363)
                     /locus_tag="JA154_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-0--gamma-glutamyl ligase"
                     /protein_id="HAT7996786.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            11482..12369
                     /locus_tag="JA154_13100"
     CDS             11482..12369
                     /locus_tag="JA154_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT7996787.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
BASE COUNT         4669 a         1644 c         1667 g         4432 t
ORIGIN      
        1 gtttaaataa agaaattcta acagttaaaa acattaccag tgataaaatt aaaattataa
       61 ataaatcagc tatcatttgc catgctaatg gttgaattat gtaagcaatt atacaagaag
      121 ctataacccc taatggaata aaaataattg acaaaatatt tccttgtaga gaaataattt
      181 tttttaactg aaaagaagtc atacctaacg atttcaactt agcatacatc tgaactgaat
      241 taacaactga aatataaaaa atactatata taacaaaaac gctagaaaaa agaacaacta
      301 ttccaactac taaaactgta ataatctgct caccatcaat taaatttgat tcaatatagt
      361 tatcatttaa gtatatatta tatttcttaa tacctaaaga taaaccaatt ttatttaact
      421 gtgtcttaat tgaagctgaa tctttctctg tattttcttc tgatagttta actaatacat
      481 ctacagtatc aggtcttgac tcgtcaaaag tattactttt attttgtaaa ggtgtagaag
      541 ctatattctt aaaataatca tttgaaacga ctgtatagta ttgcttggca ttatcttgat
      601 aataattttc tagaaatcca acaataataa aatcacattg aacttcttta ttcgtaatat
      661 catcataata atttaattta atgctatctc caatatcttt atctaaacca aattcctgta
      721 aatatttttc tgaaatagca atttcatttt tatttgtagg tagtttgcct ttcaatggtt
      781 gaaaattcat aagtttaatt gctgtatcat ctgcataaac aatcgacata atacaatctg
      841 tctttttatt acttccaatt aatttgtaag ttcctacgcc actaaagttt ttatttttat
      901 atagtatatt ttttgctttt tcatctacag atttatatat agcatgatat ggagatgcat
      961 taactaaatc tacagaggca ttatatgaaa ctaaggtcac tgttaataat aaacatgtag
     1021 aaatcattat aatgatacct gaaagatatg ttttaagttt attaattttt aaatcattta
     1081 aagctatttt tcttataaat gtgcgattat tatttctaaa catatactac acctcctagt
     1141 taacaatttt tccatcttca atacgaataa ttctatcagt tagttgtgca atttcattat
     1201 tgtgtgtaat cattacaatt gtttggttga attttttact tgtcacttta agtaaactca
     1261 gtacatctga actagttcta ctatcaaggt tacctgttgg ttcatcagct agtataatag
     1321 ctggttttgt tacaagtgct ctagctatag caacacgttg ctgttgacca ccagaaagat
     1381 tacttggtat atcatataat ttttcttcaa gagaaagcat atttataatt tcatttataa
     1441 aatccttatc tatggtctct ccatccaatc ctattggtaa gataatattt tcatatacat
     1501 taagtaaagg aacaagatta taattttgaa atataaaacc tatatttctg cgtctaaaga
     1561 ttgtaagctg ttcatcagac atagtagata attccttacc ctttataata acctttccag
     1621 atgtaggatt atctaatcct cccatcatat ttaataatgt ggatttgcca ctaccagatg
     1681 tacctacaat agaaataaat tcgccttttt caatagatag agttacacca tcaagagctt
     1741 ttgtaatgtt tgaactttgt ccataatatt tctttaaatt aattacttgt aaaatactca
     1801 taataatacc tccttaacat cttgtaaata cagtataaaa ccaaaatgtt gcagaatcat
     1861 tacaaatgaa atttttaaaa atttatttat taggtaagaa aatagaaaaa gtcgaccctt
     1921 ttccaatatc tgatgtaact ttaatatagc caccttgaag agatataatt tttcttgaaa
     1981 gatagagacc tattccaata ccttctatgt catggacatc ttcttcacga taaaatcttt
     2041 taaagatttc tgcttggcga ctttctgata ttccttttcc attatcaata atatctattt
     2101 ttgtatacat aacccaacat tctgtagcta cacgtatctt accattagaa cttgtgtatt
     2161 ttacagcatt atctaatata ttaaaaatag cttcagaagt ccatttcttg tcatgaatta
     2221 tatataaatt tgggtcacaa tcaactgtaa cttcaatatt ctttttttca gcatcaaaca
     2281 aaattccact aagtgctgta gcaattgttt cataaattga atttttcttt ttagatagtg
     2341 tgattatacc tgtctctagt ctggaggttt ttaccataga ttgcattaaa aaatctaatt
     2401 tatctaattg gttttccata tccattaaaa aatccttttg aatttctgtt ttaaatgttt
     2461 gatttagtag agtagtatta atcattttta aatttgcaat aggagtttta atttgatgag
     2521 atatatcaga aactagttct tgtaaatcag ctttctctat agcaatacta ttgcgatttt
     2581 cttgaataac ttcatacatt ctaattaatc tgtgattgat acgtgataaa atagtttctt
     2641 cttctaattc aatttcaata ttatcttcac tattcatcat actatctaaa gtgtgacata
     2701 ttttagaaca aaatataact atttttttat gaagtaaaaa tattattaat gctataaatc
     2761 cacaaagtac cattgtatat attaataata gagcaattag ttttaaatca ccagttatta
     2821 gataaattga gatactaaat aaaatagctg taaaaattat accaacagca attattaaat
     2881 aaatagaact aaaagaaaat tttttaaaat tcatttcttt cacctaacca catatatccc
     2941 ataccatata ccgttttgat atacttaaga ttgtcttttt caattttgct tctaatccta
     3001 cttataacag atgttaaagt atgttcatct acaaagttag catctatatc ccagagtttt
     3061 tctaaaagta ctttccttgt taaaatactt ttaggatttt cagtaagcag ttttaaagta
     3121 cgatattcta atggtgtaaa tataatttga tttccattta tattggcaga taactcagaa
     3181 aagttaattt caagatatcc atccttatag taatcttgtt ttgtttttat tgtaagatga
     3241 tttaaaaaag ctttaatttt ttgtttaaat atattgatat taaatggttt tgttatataa
     3301 tccaaagctc caagttcata gcctttaatc atatcacatt ccatatcatt agcagttaaa
     3361 aaaattattg gtatattgta attttccttt atttcattac ataattcaaa cccactttta
     3421 tctggtaaat taatatccaa aattattaaa tcaaattcac attcaaaaat agactttagt
     3481 gcagatttag ctgtaaattt tgaagtaatt atatatccat cttcaattag gttatatgat
     3541 agagttttat ttaatataga atcatcttct actactaata ttttttccat acagttactc
     3601 cttttagttt aatattttat aatgtcaatt atagactaaa aacattgctg aattgttata
     3661 aacgagaaaa agtatttaaa aatttatata tttaataaag aacctgtcct atgataaatt
     3721 ctagtacaga ttctttatta aataaaatta tttcaaccaa gatggatttc caggtataaa
     3781 tattctttta ccaaattttg aagtatactc atctaaagct ttttgaataa gctcattatt
     3841 acccttaaat gaagtagtat tctttatagt ttcgagattt tggctaaatg ggcaagcaga
     3901 tatacacata ccacaatctg tacctaaata tctccattta atatagcatg tctcatgttc
     3961 aattacccaa ttatattttc catcatcacc aattttaata tcattagaaa gcgaatgaga
     4021 agggcagtta aaagagcatt ttttacagac cttacaaaaa tcttctagtc caaaatcaac
     4081 atattcatct tctaaaagag gaatatctgt agtaacaaca cctaatctaa gtctagagcc
     4141 ataatcttta tttgtaagta tagcatttct tcctatatct cctaaaccag catcacgagc
     4201 tataaaaaca ggcattacta aataattagc atccatatga tttctagcat catatcctaa
     4261 atttcttata tagtaactta aaatcatgcc gacgatagat gcatccacat agcactttga
     4321 tgtttctata acttcacata tcattggagc tctatttatc atttctttat ccatctcaca
     4381 tccaaataca attgcatact tatgagttag attaatttct tcaccgtaat tttcttcatg
     4441 tctaccccta tttgtataaa tatgatagtc ttttaattta gttataccaa ctacagaagc
     4501 tccatactgc tttgcaagac ccttaatttt ttttgtcatt gactttgcat ctacatctac
     4561 tttagtatca gaaacttttc cttcgcataa agatttgata tcagataaaa aatcgaatgc
     4621 actacttgcc attggagagt ttaactcatt gtaagtcatt gttccttcac tacataaatt
     4681 tggtctattt cttatactat catcaatttc ttttttgtca ggatttttct tataatagtc
     4741 attataagca gtactatctt ttttgtaatt tgctcttgca aacatagtat ttctctcatc
     4801 aacccttttc aattctattc cccctagtaa aaatatattt aaaaaatata actttattgt
     4861 tgtataattt aattatataa gaataaagtt atattttcaa tttaagcgta attaaattgg
     4921 gaaatatata actatatgct tgattataat gtttttattt tgaaaacaaa tttaaataca
     4981 atctttttag tgaaataaat aaaattaaaa tatttttagt atgataatgc aagaaaataa
     5041 ttatttatct aaaaaatcta aaggagagta aaattgaact tattaattat tgaagatgat
     5101 ataaacttaa atgaaggtct tttttatgca tttgagaatg atggatttaa tgtatttaag
     5161 gcttatacaa aacaagaagg attaaatata tttaatagta aaaatataga ttttataatc
     5221 ttagactgca atcttccaga tggagatgga tttgatgtat gtcaaataat aagggaaaag
     5281 tcagatatac caattataat gcttactgca agagactcag aaattgatga agtaaaagga
     5341 ttagaaatag ggcttgatga ttatattaca aaaccatttt ctttatctgt attaaaggca
     5401 agagtcaaag tagcaataag aaaaaaatcg aataataaag taatatattc caatggaata
     5461 aaattggacc aaaagttact aaaagtatat aaaaataaag aatgtttgga acttagctct
     5521 gtagaatata agcttctaag ttatctaatt gaaaataaag gtcaaatatt attaaaggaa
     5581 caaattttac accatatttg ggacagcgaa gaaaattatg ttgatgataa tattgtatct
     5641 gtaaatataa gaagacttag agtaaaagta gaggatgacc catctaatcc aaaatatata
     5701 aaaacagctt atggaatggg ttatttatgg aatgaggtag aataatatgt ttatattagc
     5761 ctttataata tgtacagttg ttatatttat agtatctact aaaataaatc gaaaaagata
     5821 tgatgaatta aatttgatgt taaatcaaat attggaaggt aaagaagtta cttatccaga
     5881 tacaaaggat acaagagcgt caaaaatttc tcaccaagta aaaaaaatta aggatatgat
     5941 agaaatagag gtagaacagt caaagtcaga aaaagaagcg attaaaggtt taatatcaaa
     6001 tatgtcacat caattaaaaa ctccactttc aaatataacc atttattgtg aacttctgga
     6061 gaatataaat atttccacat tacagaagaa ggagttttta caaaagatga aaaatgaaac
     6121 ttttaagatt gactggctgt tacaatcctt atttaaaatg actaaattag aggatggagt
     6181 tatagaattt gaagtagaag aactactaat taaagatact cttattcaaa gtatcagtac
     6241 tatctttaat aaagcagaag ctaaaaatat aaggataaat ctcgaaccat tttcagacat
     6301 aaaattagtt cataataaaa agtggaccat agaagcaatt gtaaatgttt tagaaaatgc
     6361 aataaaatac tcaccatcag atagcacgat tactatttct gtaataaaaa tggaactata
     6421 tacgaagatt actattaaag atgagggaat aggtattgat agtagagaac taaatgatat
     6481 ttttaaaaga ttttacagaa gtaaaaatgt tgaaaaccaa aatggcacag ggattggtct
     6541 atatcttaca agattaattt tagaaaaaga aaatggaaac attatagtag aatctaaact
     6601 aggaagtgga tgttgtttta gtatcttctt acagaattgt aagagtttaa actaattttg
     6661 taggatgagt gtaattatcg tcacttacaa tattttatag aacatatatc tgtattgaaa
     6721 ggagaaatta aatgagtata ttagtgacga ataatttagt aaaacactat ggaaaaggtg
     6781 agactaaagt aaaagcctta aatggagtaa gtatagaaat tgaacgagat acatttactg
     6841 ctatagttgg aacttctggt tctggaaaaa gcacattgtt aaatattata ggtggattag
     6901 ataatccaac ctcaggtgat gtaataatta aaggaaagaa catatctaaa ataggaaaaa
     6961 aagatttgac agtgtttaga agaagaaata taggatttat atttcaaaac tacagtttaa
     7021 tgcccgtatt aaacacttat gataatatag ctcttcctgt tacattagat aaaggaaacc
     7081 atatagacca tgaatatatt gaaatgctga tgaagacatt gggaatttgg gataagcgtt
     7141 taaaatttcc aagcgaacta tctggtggtc aacaacagag ggtagcaatt gctagagctt
     7201 tggcaaataa acctgcgctt atattagcag atgaaccaac aggaaatctc gatagtaaaa
     7261 ctacaatgga agtggtatgt ttattaaaag aaagtagtgc aaaattccat caaacgatac
     7321 ttatggtaac acacaatgaa aatatagcac aagtatgcga ctcaattatc catattgaag
     7381 atggagttgt ggttaatact ggtggtgaat tactatgatg tctctagtaa aattttcaat
     7441 tcaatatata aagcattata aaaaacaatc tatatctata gttttaagca tagtactatc
     7501 agtagcatta ttgacgggaa ttggttcttt agttcatagt gcagacaaaa gtagaattga
     7561 aaagataagg gaagatagtg gagattatca ttatttctat aaagtagata aggaacaact
     7621 taataaaatt aaagaaaata aaaaatcaaa ggaatacact gtagatagac ttggcgttac
     7681 aaaacctaaa aatcagatag atgaaccttt tattatagaa tttttaaatg ctgattcttc
     7741 atatttagac atgacaggta gaaaattatt aaaaggaact cttcctagta aagatggaga
     7801 gatagtactt gacacatatt ctctaaacaa tttaaatgta aacaaagaaa ttggaacaga
     7861 aatcaattta gatggtaaaa tctataagtt atgtggtatt ttatcaagtc tttcaaaccc
     7921 agaaactgct ctggagggat ttgtaaacga ttattttcag cctaaagaaa aggattctta
     7981 tatagtttat gtaaaatatg atgaaaataa aaatattaaa aagcaaaatt ataaatttat
     8041 gaaaaaattc aacatatcaa aagaaaaaac ttatataaac tggtctcttt cagttgaatt
     8101 tggagtaaaa ccaccaaaaa atatagaaaa atataatata tttgagttct taaaaaattt
     8161 agaactaaat acaaatgcaa ttatattgct tgttggagtg tttggtgcag cagcagtata
     8221 tagtattttc catgtttcaa tacttcagag aatatcacaa tatggggttt tagaagtatt
     8281 aggcgctaat aataaacaac ttttatttct actattttta gagttatttt tactttttat
     8341 tgttggattt cctataggtt gcttcttagg tattggagta gcgtctacaa tatatgaaca
     8401 atttccacat atatttttga gtagtgatat tgttccagga gcatttttta taagtgaaaa
     8461 atctatttat ttaggctttt tatttatctt attgttgtta attttaattg ctgtacgtgt
     8521 tgtacatcag ttgaataaat actctagtat agaatctatg aaaaacttta agattactaa
     8581 taagaaaaag agaatattag aaagtgtaaa acactctaac atgacaaaag tattatctca
     8641 taaatacatg actcaaaaaa agggaatgtt tattggaatt ttgttttcat tatcacttgg
     8701 tggaattata tttctatgtt ctagttactc tatacagctt actaaaaata ataatgaact
     8761 tacaatgaaa gctgatgatg gtttaaattc tgactaccaa ataactatgc aaacaacaga
     8821 ttttgatgta ggtataccaa aagataaaat ttcaagatta aacaatgttg agggaatatc
     8881 aaaagtctat ccagtaagat atttatttgg agctatgcaa attaaagaag aacaactatt
     8941 ttggaaaaat ttctttaaac ctcttgaaaa agattacagg ataaagactt tttttgatgg
     9001 tatttgtaca aagcaaaatg aaggtggcta tcttttaaaa acaaatattt atggttataa
     9061 caatggtatg ttagaaggat taaattctta tattatagat ggggagatta atacccatga
     9121 tatggttaaa aataataagg ttatagttag actacctatg gatggaactg ggatgtatga
     9181 tgcagttgat ataaatccag gagatacaat aaaagttaaa gtgccaaaga caatgaaacc
     9241 aacagatgaa attgttaaat ttaaagaaga aaataagaat gattatacaa caaaagaatt
     9301 tgtagttgct gctactgtaa aacgtgtgat ggcaaataac atatatttca ttggtgatta
     9361 tggtatggat atagttatga cgaatgggca aatgaaaaat aactttgata tcgaaaatta
     9421 taattcagta agtataaaaa agacagaaga tagtaacagt gaggaattgt ctgagaacat
     9481 aaaaagtgtt gtaagtgatg taaaaagatg tatcgtaaca gattatacca ttgctattga
     9541 aaaaaataat acctacttaa atcaaaaact cttatttata tatggtattg tatttgtact
     9601 tcttgcaata agtttgttcc atataatcaa tacagtaagc tacttaattt tttcaagaag
     9661 acatgagttt ggaattttaa gagctatggg tattacggat aataagtttt tacttatgat
     9721 gataagagaa ggttttttat atggattcta tgcaagtatt ataatggtaa ttggaagtgt
     9781 tattggacaa tttatgattt attttatggt aaaacgtgta tatttatata taaatccaat
     9841 tcttaaaata aatacacctc tatacatagg tatgattatt ttaaatatca ctattagtat
     9901 agttgctgta attattccag taagacaaat tttgaagagt gatattattt cagaaataaa
     9961 taagaattag agttatataa atttttaaga ttatataact ttctgacaat cttgtttaaa
    10021 ttatttaaat atatcttatc tgtgaacatt aaataaacct cttaatagat ataactcttg
    10081 aaaagtcata tctattaaga ggttttaata aattttcatg ttaatatata taatccaaaa
    10141 aaattaaatc ttattaaaac atactatttt gtatagttat cgaaatatcc ttgtatgtaa
    10201 ataataggag tacccttatc tccacttcca gatgttaaat ctgctaaaga acctataagg
    10261 tctgttaatc ttcttggagt agtaccttga gaaaccatgt ttcctgttaa atcatcagat
    10321 ttattatctt tttcaactat gtattttgaa attgcttctt ttaactcatc accagataag
    10381 tcagcaaaat cattatctgc taagtatttt aattttactt cgtttggagt tccctctagc
    10441 ccttttgtat aagcaggaga tactactggg tcagctaatt cccatatttt acctacaggg
    10501 tctttaaaag ctccatctcc ataaaccatt acttctacat ctttccctgt tatttctttt
    10561 atattacctt ggattttgtt tacaacttca tcacaattta tagggaataa ttttactgtt
    10621 tcttctgtag ctttgtttga acctaaaagt ccatactggt cattgtaacc actaccatct
    10681 acacttgctg tcattatatc atcaagtgaa tatacttttt tagctccatt ttgtcttaaa
    10741 attctctttg ttctttgtct tgtatgtata tcacaagtta aaatacttgt tgtatagtta
    10801 agaatagttt ttggattgtt tgcaaatact atttctacct ctgcaccaca atccttgatt
    10861 aaattcttat aataatcaac ataatctaca ccagtaaatg tatgcttgtt atatccaaat
    10921 agttctctgt atttttcttc tgttaaaaca tctgaccaag gatttattcc cttatcatca
    10981 agttcatcaa tacttattaa gtgatttcca acttcatcag aagggtagct taacattaat
    11041 acgacttttc tacatccttt tgcaattcct tttaaacata tagcaaaacg atttctactt
    11101 aaaattggga atataacccc aacagtatca tctccaaatt tatcttttac atcttttgca
    11161 atattgtcaa cgtgagcata atttccctgt gctctagcta caacagcttc tgtaacggca
    11221 attacatctt tgtccctaac ttcaaagttt tcactttttg acgcatttaa aacagaatca
    11281 acaactattt taactaagtc atctccttgt cttataatag gtgctctaac ccctcttgaa
    11341 acagttccaa ctaatctatc cattatctaa aatcctccta aagaattaat attgtcatat
    11401 tagtaccatg tatattataa ttcaaatata attataagta aaataaataa tactaatatt
    11461 tgacataagg agtaattata tatgaatata aaacttgaac tttataaggt ttttaatgca
    11521 gttgtaaata ataagagttt ttctcttgct gctaaggaat tgtttatgtc acagccagct
    11581 gtaagccagt caataaagca attagaagaa caattagata ctttattatt ttatagaaac
    11641 aataagggag taaaattaac tccagagggg aaaattctaa gtgaacatgt cactactgca
    11701 ttaaagttaa tatcatcagg agaagataga ataaataaat ttaaaaaatt agaatacggt
    11761 tctctgaaaa taggagtagg agatacagct gcacgttttt tcttgttaaa atatttagaa
    11821 atttttcata agaagtatcc acatatacat gtatctacaa taaatagaac gagtagagaa
    11881 cttatatctt tactcaaaga tgggaatata gatatagcaa ttataaacat gccaatagaa
    11941 gatgatacac taaatattgt tgagtgtatt gaaatacatg atatatttgt atgtgcaaat
    12001 gattacatag aatacaaagg taggaaaata tcattggaag aattaaacac attgccttta
    12061 ataatgctag aaaataaagc aaattcaaga ctttatgtaa atgaatattt tttatcaaaa
    12121 ggtataaaac taaacccgga tatagaattg ggttctcatg aattactttt agaatttgcg
    12181 tatataaatc ttggagtgtc ttgtgttata gaagaattta gcatagatta tttggaaaat
    12241 gagaaattgt ttaaattaga tataaaagag ccaataccta agagaaatat aggttactgc
    12301 catttaaaag atatatcttt atccttagca accaaagaat ttttaagcat gatatctaat
    12361 aatatataat ttagaataca tatttcacaa gtaatttaat ataaaaatag ag
//
LOCUS       DACULQ010000119        11956 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.120, whole genome shotgun sequence.
ACCESSION   DACULQ010000119 DACULQ010000000
VERSION     DACULQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11956
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.120"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..279)
                     /locus_tag="JA154_13105"
     CDS             complement(<1..279)
                     /locus_tag="JA154_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HAT7996788.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIF"
     gene            complement(308..1858)
                     /locus_tag="JA154_13110"
     CDS             complement(308..1858)
                     /locus_tag="JA154_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996789.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            complement(2060..2944)
                     /locus_tag="JA154_13115"
     CDS             complement(2060..2944)
                     /locus_tag="JA154_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT7996790.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ANKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            3172..4185
                     /locus_tag="JA154_13120"
     CDS             3172..4185
                     /locus_tag="JA154_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HAT7996791.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLFLLYFFSNNLI
                     YAQNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYS
                     DFANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTS
                     EFGAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENT
                     DFGIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKH
                     PESKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIK
                     WML"
     gene            complement(4401..5099)
                     /locus_tag="JA154_13125"
     CDS             complement(4401..5099)
                     /locus_tag="JA154_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HAT7996792.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            5313..8006
                     /locus_tag="JA154_13130"
     CDS             5313..8006
                     /locus_tag="JA154_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAT7996793.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFKENNEWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEILGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            complement(8280..9215)
                     /locus_tag="JA154_13135"
     CDS             complement(8280..9215)
                     /locus_tag="JA154_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HAT7996794.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(9259..10404)
                     /locus_tag="JA154_13140"
     CDS             complement(9259..10404)
                     /locus_tag="JA154_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HAT7996795.1"
                     /translation="MNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPELAFEEYKSQ
                     KALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPGMGHACGHNI
                     IGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFNNVDAALILH
                     PSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSVNSMRLHLKD
                     YARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKGAAISTGTNV
                     EIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGNLTHEIPAIQ
                     AYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            complement(10418..10837)
                     /locus_tag="JA154_13145"
     CDS             complement(10418..10837)
                     /locus_tag="JA154_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996796.1"
                     /translation="MFIYIIICFGQMIALKTQAQDLLIGSVSGFLLVIIAIIIKNFVK
                     KPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTPLLAFAGISVGNQLDI
                     LKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            complement(10897..11739)
                     /locus_tag="JA154_13150"
     CDS             complement(10897..11739)
                     /locus_tag="JA154_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HAT7996797.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
BASE COUNT         4053 a         2015 c         1560 g         4328 t
ORIGIN      
        1 aaatatattc ctgacttctt caaaatgtat cttcccttgt ggagaagatt ttaatgttgg
       61 taatttgaac tcagacacat tcttatttaa gttataatca attagataat cgtagttcaa
      121 ttttatatca tctttagata aaccagataa ctctgatata tttaagtaat caatgtcaaa
      181 ataataaagt tgtttaaatc caacagtaaa attaataaca cctactatta taacaatact
      241 aatacaaatt gaaaaaagta catttagaaa ctttttcatg aaatcccccc agcaataagt
      301 taaatcacta cacgatttta gttgtaaaat cttctatatt ccatacatca tcaacaattt
      361 ctgaataaaa ttctggctca tgacatacta aaagaactgt tcccttaaac tctttgatag
      421 ccttcttcaa ttcatctttt gcctcaacat ctaaatgatt tgtaggctca tctaatacta
      481 agaaatttat attttttaac attactttgc aaagtctaac cttagcagct tctccaccac
      541 ttaatactct catttgactc gttatatgtt catttgtaag tccacatttt gcaagagcct
      601 gtctaacttc aaagtttgta agccctggaa attcactcca aacttcatcc ataggtgtat
      661 tactattttc tcttgaactc tcttgttcaa aatatccaac ttccaaatag tcacctaatt
      721 taacatttcc ttcaaatggt tttattattc caagtaaagt ttttaataga gtagattttc
      781 caataccatt cattcctttt aaggcaatct tttttcctct ttctaaatgg aaattaagtg
      841 gttttgtcaa tgcttcatca taccctaaaa ctaaattttc tgtttcaaaa attattttac
      901 tagctgctct agcatcttta aaagcaaaag taggttttat tttttctttt ggtctttcta
      961 atatttccat cttatcaagt tgcttttgtc tactgttagc cattcctcta gttgctactc
     1021 tagctttatt tctagctaca aaatcctcta gtctttttct ttcttcaact tgtttatcat
     1081 aagcttgttc ttcttgacgt ttctttatat cattaagtct tacaaattca tcataatttc
     1141 ctttatatct atttaactct ccattttcca tatgatatat tacattacat gtattattta
     1201 taaattcaat atcatgagaa actaagacaa aactattctc atactcttga aggtacttag
     1261 taagccatgt tatatgctct tcgtctaaat agtttgtagg ctcatccaat ataagaatag
     1321 ttggattttc aagtagaagt ttagtaagaa gtacttttgt tctttgccct ccacttaagt
     1381 cagttacatc tttatcaaga ccaatttctc caagaccaag accatttgca acttcttgta
     1441 tctttgcatc tatcatataa aaaccactat tttcaagtat agtttgtatc tctgctgttt
     1501 cttcaagaag cttactcatt tcatcatcac tagcttctcc cattttgtca tacatagcaa
     1561 tcatttcttg ctctaagtca aacatatgct taaaagcctc tctaagcact tctcttatag
     1621 tttttccctt gcttaatacc gtatgttggt ctaagtaacc tactgtagct cttgaagacc
     1681 attttatatt tccagcatct ggcataagct tctttgttat tatatttaaa aaactcgatt
     1741 ttccttctcc attagcacca actagtgcaa tatgttcacc ttttctaagt ctaaaggata
     1801 cattttctaa tattgttctc gcaccaaatc catgactcac attttctact actagcattt
     1861 caacacctct caaaaaaatt ttacacattc tatactataa tatattcttt ttaaacttaa
     1921 atcaagtatt cccaatattt ttagttatat ttacgacaat ttaacctaaa ataaacttct
     1981 tctttgtgtt atgtacaata aaaaatagag ataaaaatta ggaaaataat accaacctaa
     2041 ctttttctct attcatatct tatttaaaat acgcatccaa tccatcagct atacactgca
     2101 tcatttttct ttggtagtct ggatttgaca tcatttggtc ttcattatag ttactcataa
     2161 atcccatttc tactaataca gctggtactt ttgaccagtt gaatccagtc aaatcacctc
     2221 tttgaacgat tccattgatt tgtatacctg attgttccat attctgtttg ataaattcag
     2281 cacatttatt actttcttca tatataggag ctgtatattt tccatctttt tcaggaatca
     2341 atatagatgc tccagttttt gaagaattat ttaaactatc tgcatgtatt cttacaacca
     2401 tatctgcttt tttgtcatta gcaagtattg ctctttctga attacttata tttacatcat
     2461 gtgtctctct agtcattatt acattataac ctttagattc taatatactc tttaaaacaa
     2521 gagatgcttc taagtttaaa acatattctg gcttttttgt agcaataccc tctgttcctg
     2581 aagatactct tgctttttta gaggaagaac ctggagctac tggttctaaa ttgctatctc
     2641 cttttccttg atgccctggg tctatacaaa ttaaaagttt tttatttttt tgaatttcat
     2701 ttttattatc tgacgatttg tcctctgatg acttatcttt attactttta gaattagatt
     2761 tgtcttcatt tttatcttta ttatttttgt ttgaattttc atctttattt gctttttcat
     2821 ttgtgatatt tcctgaacta atcaaatttg tagtcttgta aaaattgcta actggatttg
     2881 atgttcctat aattatgatt cccatggcaa ttatacaagt aactaacaat ctatattttt
     2941 tcattttttg ctcctttctt atgctagaca gttattgttt atttttattt atatgaatat
     3001 gtatttattt tatcatatac actatttatt ttatttattt ttatgaaaac taaggtgtta
     3061 ttgagctagt tttccaagca tcaaaacatg attcttgcac aacataatat agaagcagca
     3121 agaaaacttc aattctatta ttaaaataat ttaataagaa agaggtgttt tatgcaactt
     3181 aaaaattttc attttaaaaa taatacttta agtaaaaaaa ccaacttagc taagtcaaaa
     3241 tttacaattg ttttaacatt tttattttta tttttattat attttttctc aaataattta
     3301 atttatgcac aaaatttaca aaaaaatgaa cctgaatatt taataccaat tggaaacgta
     3361 cttcaaattg acgcagaatt aaaaaatatc atagttagaa atcctggaga agcatctcca
     3421 tttagattgg gtgatgctat agtaaaggta aataatgctg aagttgatgg atatagtgac
     3481 tttgccaata ccttaaatgc tttaccagaa gaaaaacaag tatctatttt attaaataga
     3541 ggtggacaac ttataacaat gaaaactacc aaacacatat tagaaaaaat tagttttaac
     3601 aatttacttt ctggatttgc tactttaacg tatatcaatc cagatacatc agagtttgga
     3661 gctgtaggtc atccaataag tgttggtaat gcaagaaaaa tacctattaa aacaggttca
     3721 atttctacaa ctaagcacct taatatagaa aaatcttata aaggtagtgt tggatgtata
     3781 aatgcaaaac gagaaaatat tattggtagc tttacagaaa atactgattt tggaataaga
     3841 ggtcaaatta ataactttga tatttccaag ttaaaaaaat ataaagtagc atctttagat
     3901 gaagttaaat taggaaaagc acaaataatt ttacaagata actttaataa gtgtaaaaaa
     3961 tataatattg aaattataga tatagaaaac caaaaacatc cagaaagtaa aagttttaaa
     4021 attaaaatta cagataaaaa gcttttagct caaactggtg gtattgtaca aggtatgagt
     4081 ggtactccaa tagttcaaga taataaaata atcggagcag tgtcacatgc aattgaaaac
     4141 gacccagctg taggttatgg tgtatttatt aaatggatgt tgtaaataat ataaataaat
     4201 aaataagttt taagatgtta ttaagttatt aataatatat ttggtctaaa ggtactttta
     4261 tttaacctac agaccaaata tattagatat tttacatagt ataaaatata aatttttatt
     4321 aaaattatat taatattatt tccaaaaatt ttatttataa actattattt tgaatttatt
     4381 taaaaatata caactttata ttacttgttg gtattctttc cagtgttagg taaaacattt
     4441 cctagatttt tttctggaat atttacataa ctttctggaa catcacctac tattatagtt
     4501 tcacaaatag gtatttgagc tttaatttgt tttgttgaag ttataagtgg tattactact
     4561 ttaacttgtg tttgagcttc taaatatatt ctatgtctag tttgatttat acctgaagac
     4621 tcaaattccg ttttaaagtt tactgatact gttcctatag gttcaataga tacttcaagt
     4681 ttaggaccgt atttagctaa tataggactt cctaatgctg ttcctatagg tatgtaagaa
     4741 cttgctgtct tatccttttt taattcatct tgtatttcta aagctattgc agatgcaatt
     4801 tcattcatca ttatagtgtt tgcttgtatc atagttattt taccattatt atcaagttgt
     4861 atatccatta aatcttcata atttattttt ccttctacca tctcagcaac agctttattt
     4921 atcgacctat ttgctagttc aagagcttta gtctctgcta aaactgttat tgtcggtctt
     4981 aaagttttat ctatatatat aaaactgcct ataaatacag acaagaaaag aattactaaa
     5041 aaaatagcta tgatttttct aaattcattt tttgtttttc tatctactat tctattcaaa
     5101 attatcccct cctatttatt tatatgtagc tttggcataa tttttataat atttgtcaaa
     5161 aataaataaa ataaaaatat agacttttta atatattacg tttatacata tacaatgtac
     5221 agaatattat agcaaattca ttttttctat taattacaaa actactccta tttgtgttat
     5281 aatattgcat taagtaagga ggttaaaatc ttatgaataa cgataatgat aaaaatggaa
     5341 ataaaatcag gagaaaaaaa gttagctcat ctggtaatac aaataaacca attaatagaa
     5401 cttcagctaa caagactcgt tcaacaaaaa acaagaaaaa atctaaaaaa agcgataaat
     5461 ttaaaaaact aagagtattt gggatagtat ttttagtttt actagttgtt ggtacagcag
     5521 gtactgctgg tctagtattt gcgtcactaa gagatgtaac tccagttaca gaagctgttt
     5581 tagataaaca gacgaaccaa acaactacta ttaaatacgc taatggaaaa acattatcta
     5641 cagctccaag tgtaaataaa aagacacccg ttccattaga taagatttcc ccttatcttc
     5701 aacatgctgt aattgcaata gaagatgagc gtttttatga gcataagggt gtcgatatta
     5761 aaggtttatt taggtcagta ttaaagaccc ttacaggtac taagcaaggt ggtagtacta
     5821 ttcctatgca ggtatccaaa atgctgttaa caactgaaca gcaaactata cctcgtaaaa
     5881 taaaggatat ttactatgca catgagatga gtaaaacagt aagtaaagat aaaatactag
     5941 aaacttattt aaataacttc ttcgttggta gaggtctagc aggtgctgag gctggtgcac
     6001 gtggttattt tgataagtct gcagctgacc ttactctagc agagtcagca cttcttgctg
     6061 gttcaactaa aaatccatca agattttcag cctataaaac ttctaagtta gagggaaatg
     6121 aaactaagga ggatttagag aataaattat tattttttgt taacacaact gatgatgatt
     6181 tagatgaccc tactcaagtg gactttgata tgattgaaaa aataaaatct tgggagctta
     6241 tatctaatga tacttataga caattaaaag caggtacatt agttgttaga aaggctgtaa
     6301 gtaatcctga agctaaaaaa agacaagaaa ttgttttaaa gaaaatgtta gaattgaaat
     6361 atataaaaca aagtgaatat gatgaagcaa tcaaagctaa aatagaaata aaattgcctc
     6421 aatcatcaga taaagtttct tcatctgtag aagaccttat cgaaagtgaa gttataaatg
     6481 ctttaatgga acaaggacat acaaatgatg aagctcaaaa cttattctac aatggtggtc
     6541 taattgtaaa cacaactata gaccctaaaa tgcaagatgc tttagaagaa gaatttgata
     6601 gaaatagcaa tttcccagga catatggtcg gtcctgatgg agttagccaa ccgcaagctg
     6661 caatggtaat cttagattat aaaaatggtg aaataagagc actagctgga ggaagaaata
     6721 taagtggtag aaaaacttta aatcgtgcta ctaaccctca tcagccaggt tcttctataa
     6781 aacctttagc aatatatacg ccagctattg atactctaaa aataacacaa gcaactgctc
     6841 taagtgattc tcgaggagga tataagttta aagaaaataa tgaatggaat ccaagaacta
     6901 ctactgctgg tcatggttct atgagtttac gtaaagcact tgctaaatct tcaaacacga
     6961 tagctgtcaa aactgctgaa atacttggag actcttatga tgaatgtgta gatataatga
     7021 tggattactt aaagaacttt ggtataacaa ctttaaagaa taatcactca ggttcatctg
     7081 aagcaagtga tagaaaattc ccatcattga cactaggtgg tatggcgaat ggtattactc
     7141 cacttcaaat ggctgctgct tatggtacat tggcaaatca cggtatttat gtagaacctt
     7201 ctatttttac tactataact acctttgatg gtcaattatt agttaaaaat gctcctgaag
     7261 aacataaagt tgtagaccct gaagtagctt atgttgttac agatatgtta gaaagcgtaa
     7321 ttactgaagg tactggtggt gttgctacac ttccaaaggg aatgcctgta gcaggtaaaa
     7381 caggtactac aaatagtgca tatgatgctt ggtttgtcgg ttacactcct tattatgttg
     7441 gtgcaactta tattggagat gatgctggta gaaaagatga ttctggtaac actataaaac
     7501 gtagagaagt cccacatgga agtactagta ctgcaaaact atgggaaaaa attatggaaa
     7561 aaatacatgc taatttaacg gttactgaat ttgaagttcc taaaaatgta tattttacaa
     7621 aaattaattt agaggatgga ggaaagcaat cttctggctc taaagcagca tttattgaag
     7681 gtactgctcc tactaaagta agttctcaac catcatctga agatacaaaa aaaccagaca
     7741 ataaccaacc agaagaaaat aataataaca ataataacac aggtgataat ggtggcggtt
     7801 ctacacctcc tgataatggc ggaaacaatg gtggtggctc tactactcct cctgataatg
     7861 gcggaaataa tggtggtggc tctactactc ctcctgataa tggcggaaac aatggtggtg
     7921 gctctactac tcctcctgat aatggtggaa ataatggtgg tggctctact actcctcctg
     7981 atggtggtgg aactccagca acttaataaa aaatagaatt taaataaaaa tataaggagt
     8041 tgtctcaagg tgattaaaaa aatcactttg aaacgctcct ttttttataa ttacaatata
     8101 ttatttgtat tcataaaaaa gagaatccca ataaccagaa tcctcctttt tatataaaca
     8161 aataaatatt ttatttttaa agtttttaac taagtgcttt attcttactt ttattcctaa
     8221 ttgtttatat attcttaaat agttatatca caaattatat aaacttaata tttttctaat
     8281 tactgtatac caaatgtttg aaatctattt aatttgccat atctcttaat aagcatttcc
     8341 cactcttctt tatcatagaa atcacactta tttctaccaa atagcttggc aacttcaatt
     8401 gcaaatctcc cagctgaatc taaatcacta aaattagttg cacctgtagc acatccagca
     8461 acaggcatct ctgttgtaat tgctactcct actattggtg catctgtagc tactgctggt
     8521 tgtaaaatac tatttaagtg atacagatta tttccatatg gtgttatatc ttgaatagac
     8581 aaagggaata caaatggaga tttccctgtt acagtctgca taatatctaa aaggttatca
     8641 ctagttttta atatgtagcc ttctttaaca gttggcgata ttgcaaatcc tctagtattg
     8701 attactctat ttcctttagt agtatctata gaaagtattg catcacaact actatcaact
     8761 tcattttcat tcatagtcat catatcaatt ggagaatcca taaaaggagt aggatagtgt
     8821 tctttagttg gagcatctgg gcaaatatgg gttgaaatta caacatcacc atctaatata
     8881 tccccttttc gttgcatatc caaaagttta gcagccactg caattgctac caatgctcca
     8941 tcaccatctg aaacaaaacc tataacttct ggtcttgctc caatccctcc tagtctacct
     9001 aaaatgccaa gagtaggagc atctccacct tttaattttc catttttccc aggaattgta
     9061 agctttacta aatctgttga gcctttactt gaagacaatt cttttacagt tacatctact
     9121 tcaccataac cttgtaagta agtttttact tcttccccat tagcatttgc tttatctaaa
     9181 atgtcataaa cctctattac ttgttttaat atcatgtcta atccgatttc ctttcttaat
     9241 atattttaaa attatatatt atttactata caaaatatct actccacaca ttgctaacac
     9301 cttaattgct tttataagtg ctactctgcc ttcttcacca ccagctgcaa cagcaaattc
     9361 atctgtatga gttgcagtat tttcttttaa ttttatatat gcttgtattg caggtatctc
     9421 atgagttaaa tttcctgtat cagttgaacc tataccttga cttaaatctc tttcaacata
     9481 gtcttcaccc aaaacttcaa aattttgtct aactatattc actaattcag aatcattctt
     9541 aatctctttg tatatttcat ctaattgaac tatttctaca ttagttccag tagaaattgc
     9601 agctccttta gcacaatttt taagcatttc acaaatctca tttaaatatt ctatgcttaa
     9661 agctcttaca ttaaatatag ctgtagattt ttcaggaatt atattatgta tgctacctcc
     9721 atcagttata attccatgta ctcttgcata atctttaaga tgtaatctca tgctatttac
     9781 actgttaaat agtgctatta ctccacttaa ggcacttttc ccttcccaag gaaaagcagc
     9841 agcatgtgat gccttaccag taaaatcaaa ttttaaatta acttgagcaa atgatatatc
     9901 atcaggcatt gaagcatcac ttggatgtaa aattaatgca gcatctacat tattaaatac
     9961 accttcttta atcatagtta tttttccacc tactctttct tctgcaggtg ttccaaaaac
    10021 ctttacagtc ccttctatat tatgtttttt catgatttct tttaatatga tacctgcacc
    10081 aacagaactt gtccctatga tattgtgccc acatgcatgt cccattcctg gaagtgcatc
    10141 atattcagct aaaaaagcaa ctgttttccc attagttcca tttgaataca cagcttcaaa
    10201 agatgtttct aatcctccta cacctttagt tacattaaat tcattttctt ctaacaaatc
    10261 acataatgct ttttgtgact tatactcctc aaatgctaat tctggatttg aaaataaata
    10321 gtcagaaact ttttcaaatg atgtcttttt ctcttcagat aataacttta tttcctcttt
    10381 aatttttgct tttaattcat tcatatttac tacctcctta aagcattcca tttaatctta
    10441 aaactgtttg agatataaaa gcagaaccaa agtaagtaga tgtcataact ataaatgata
    10501 taactactaa cttccaactc aaagtcttta atatatctaa ttggttacct acagagattc
    10561 ctgcaaaagc taataatggt gttactgtag acataaaatt gattttccct acatttgttg
    10621 ctatgatatc ccccactgga gatattggaa gcgataaaac aaatgcaact aatgtagccc
    10681 atgcaaaacc tggtaaatta ggttttttaa caaaattctt tattataata gcaattatca
    10741 ccaacaaaaa tccagataca cttccaatca acaaatcttg agcctgagtc ttaagtgcta
    10801 tcatttgtcc aaagcaaata ataatatata taaacaatac tatttttatc tgctccataa
    10861 tagtcttaaa agttttttcc atatatatat cctcctttac aatttattac ttgttcttct
    10921 tgtttttctt cctctaaatc tttcaataac tggagctact ttgctataat agtagttggc
    10981 tactggtaaa gataagaata attctatata tattccagta acagctgtaa tcaatccact
    11041 agtagctgaa tatgctaaaa tctgctctga catttgtgga accgtattca ttaatgatgc
    11101 tgttgcagct cccatcattg aaccactacc cattcctgat gccattgcta aagcatatgg
    11161 atgaaatcct aaaagtacac ccactggtgc taaaaatgaa aataataatg ttccaaatat
    11221 actaccactt atgtaagttc ctagtgttcc tataccttct ggtgagttta atccatactt
    11281 atctccaata attcctagtg ctgtgtcacg acataatgaa aaagtaccac ctaatgcact
    11341 tcttccaaga cctaatgcta ctccaacagg taatgctatc aacattgtac ccatatgacc
    11401 aaattcttgt aataaaagtg ctggtcctgc ttgaagtaat ataccaactt taggccctgc
    11461 attaattcca aacattatcc ctaatggata tagtgtcaaa gataataaat atggagatac
    11521 atttatttca ttatccccta taaacttttt taaggctggt ataactctcc ctaataaatg
    11581 aggagaaaca gccattccta tgaatatagc ccatagtata ctaagtaaag ttacattaat
    11641 tcccataact ttaaatgaaa taggtccaat taattctgcc aatattgtga ttataaaggc
    11701 tgctattatg gcatttctaa tccctgcttt ttctttcatt tctatacctc cttgtaattt
    11761 ttataatttt cttataatat aattgttagt gattttatta tataacctat cattgataat
    11821 gtataatata tatttattca tcatttttat atacttttag ttatgaatca aaaaaatatt
    11881 atagattcat gatataaaat acaaataatt tattaattta tattgttaaa ataacggatt
    11941 ttcaaataat tgaata
//
LOCUS       DACULQ010000120        11872 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.121, whole genome shotgun sequence.
ACCESSION   DACULQ010000120 DACULQ010000000
VERSION     DACULQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.121"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            36..419
                     /locus_tag="JA154_13155"
     CDS             36..419
                     /locus_tag="JA154_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAT7996798.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            421..771
                     /locus_tag="JA154_13160"
     CDS             421..771
                     /locus_tag="JA154_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996799.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFVDKK"
     gene            complement(872..1330)
                     /locus_tag="JA154_13165"
     CDS             complement(872..1330)
                     /locus_tag="JA154_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996800.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1375..2163)
                     /locus_tag="JA154_13170"
     CDS             complement(1375..2163)
                     /locus_tag="JA154_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HAT7996801.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(2302..2739)
                     /locus_tag="JA154_13175"
     CDS             complement(2302..2739)
                     /locus_tag="JA154_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP deaminase"
                     /protein_id="HAT7996802.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            complement(2973..3107)
                     /locus_tag="JA154_13180"
     CDS             complement(2973..3107)
                     /locus_tag="JA154_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996803.1"
                     /translation="MGILEGAAMIREIAIKIAKEKGITEQKAWPEAVKEFKEKYELVL
                     "
     gene            3561..4070
                     /locus_tag="JA154_13185"
     CDS             3561..4070
                     /locus_tag="JA154_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7996804.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGRLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4097..4246
                     /locus_tag="JA154_13190"
                     /pseudo
     CDS             4097..4246
                     /locus_tag="JA154_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            4484..4915
                     /locus_tag="JA154_13195"
                     /pseudo
     CDS             4484..4915
                     /locus_tag="JA154_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(5102..5295)
                     /locus_tag="JA154_13200"
                     /pseudo
     CDS             complement(5102..5295)
                     /locus_tag="JA154_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            5397..5903
                     /locus_tag="JA154_13205"
     CDS             5397..5903
                     /locus_tag="JA154_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAT7996805.1"
                     /translation="MKNEKKITVALLIVYLLILSWIILFKLQFSFSALDHIRQINLIP
                     FGGSVIANGKIDFDEIINNAIVFIPVGAYFSLLFKNKSVLKAIGSVLGISFVYEIIQF
                     IFAIGASDITDLISNTLGGIIGIALVYVLSIVLKDKTHKILNRIAIVCTVLVVAFLFM
                     LLAVNNIF"
     gene            5908..6184
                     /locus_tag="JA154_13210"
                     /pseudo
     CDS             5908..6184
                     /locus_tag="JA154_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
     gene            6453..7025
                     /locus_tag="JA154_13215"
                     /pseudo
     CDS             6453..7025
                     /locus_tag="JA154_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            7128..7943
                     /locus_tag="JA154_13220"
     CDS             7128..7943
                     /locus_tag="JA154_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7996806.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALNFSNNAIIEQLDRKSIEIMDNIQKEQEKLKKIE
                     IAKKEVLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            8024..8500
                     /locus_tag="JA154_13225"
     CDS             8024..8500
                     /locus_tag="JA154_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAT7996807.1"
                     /translation="MTTYKVNSIGTICNNEKGAFIQLEAPYILALRALDGFSHINVLW
                     WFSDFDNTNFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSAVEIIDIDYMSGLI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPQVPDWCSHWPHSLEESASFAWESEFNF"
     gene            8514..9077
                     /locus_tag="JA154_13230"
     CDS             8514..9077
                     /locus_tag="JA154_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAT7996808.1"
                     /translation="MSLQDYIFEKPVIETEHLILRTMNKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPELLFDKSKKSTKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKHYQGNGLMSEVLKNVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITRWCYG"
     gene            9332..10243
                     /locus_tag="JA154_13235"
     CDS             9332..10243
                     /locus_tag="JA154_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7996809.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKR
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     SEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEIWIIPV"
     gene            10321..10755
                     /locus_tag="JA154_13240"
     CDS             10321..10755
                     /locus_tag="JA154_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996810.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            11223..11872
                     /locus_tag="JA154_13245"
                     /pseudo
     CDS             11223..11872
                     /locus_tag="JA154_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
BASE COUNT         4211 a         1740 c         2039 g         3882 t
ORIGIN      
        1 atgtttagaa atattataat aaggagcgat taaacatgaa tctatcgaag aaaatagaag
       61 ataaaagaaa agctaaacag agagctgaaa aagttaagaa agctaaaatt gctacagctg
      121 gtgtggtatt aggagctgtt actggtgcag tgagtggagt acttttggct cctaaatctg
      181 gaaaagaaac aagagaggat ataaaagatg cttcacaaca aattgctgaa aagataaata
      241 tgaaaactgt agatgtaaaa ggcaaagtat ctgagaagct agaggataaa aaaggaaact
      301 ttatagaatc aaagaaaaaa ataaaaaaat atatagataa caaaaagagt gttgaaaatt
      361 ctagtgagga agataaaata atagatatag ttgaaccagt tagtgttact gaggaataat
      421 atggacaata caataacgat taacataagt ataatatatg tattattagc tatacttgct
      481 tgtattgctt tagtttattt aattattgta cttgttaaac tacataagat tttaaataat
      541 gttggaacta tgttaaatga aaatactcaa aatataaata caactttaaa taatcttcct
      601 aatgttgtag aaacttttgg agaagtagga gaaaatatga aagatgtaac agaagtagtt
      661 actgaagtta cagcagattt tcttgttact aaagacagtg tcaaatcaaa tatggatgtt
      721 atagttgata tattgactat aattaaaaac atttttgtag ataaaaaata ataagttaat
      781 ataaaataag cctataagat ataatttaaa cgaccttaaa atagcattta taaggtcgtt
      841 ttttattagc ttgatttgac actatgtgtt tttaataatt ggtaatattc ctctttatca
      901 tttgaacata tatatctatg cttgttgttt caattacatc cataagaaca tctttacatt
      961 caagctcaaa atcttccatt ccttctaaat attcatcatc ttcatcaaaa aagatataag
     1021 aattatatag ccattcttca aatttttctt caagtatatc ccaatacgaa ctccaaaata
     1081 atccacaacc tgatatataa gatgctctac tttgatttat ataaacttca tttagaagct
     1141 tttgtaatct actgctataa agaaaatctg ctcttccact gcttgatatt aattcattta
     1201 aatcttctat tgacattaat tcaactttac cacttttatc ataaggagta taattttcat
     1261 caacccattt atgtggtttg ttttctctgc atactgttgc tatcaactct acaacttttg
     1321 ttcttatcat ttttttatcc taacttattt attttttata aaataatcat gattttataa
     1381 tttatctatt tcttgtaatg ctaatttaac agtttcatat ctatcagcac catttattac
     1441 tacatcacct tttacaagtt tattagcttc tccacctaca acataaacct ttttacctaa
     1501 accttgctta taactagacg cttcaattat taaagaatct ttcaattgcc aatgtaaaat
     1561 ctgagcagcc actttatcag catcacctgt ataaacgatt acattttcat acactttatt
     1621 ttcccctccc ccttgagaat ttgatataga tttgtttagt ataccttctg ctatcaattt
     1681 agcaacttta tctttattat taacataata atctgtatct gctttactat ctacaaaaca
     1741 tacttctatt aatattgctg gtgcaatagt atgattaagc caataaagac ctctaacatc
     1801 agactttgca cccctatctt tgaatactgt agctaaccta gtattcactc tttctgcata
     1861 ggttttgcca ttattagtct tatatattgt ttctgtccct actggagttg aagttgtagc
     1921 atttgaatta aaatgaatct gaacagccaa atcagtttcc tgtttatttg ctatagcaca
     1981 ttggtctgct aaatgattac ttgattcatc aactcttcct gtataaacag tagctccacc
     2041 tattttaagc catttaacaa ttaagtcatt cagtattctt gtttctttgc tttcgtttat
     2101 atacccaaca gctcctgtac cttttccaat taaagtgtgc cctggtatta ttactacttt
     2161 caaactaaca tctcctttac aattaaattt aacttctatt taatatatgc ataacctaga
     2221 attttgtttc tttttatatc ttatactttt attataaccc attttgtaaa aatatagaat
     2281 attaagtaaa taatgttaaa attataattt tgtatataag acattacttg attgcaataa
     2341 ttttatagca tattcatcta ttctatatcc agttctataa tatatatgtt taattccagc
     2401 ttgaattata gccttagtac aatttaagca tggaaagtga gttacataaa tagagcaatt
     2461 tttaactgaa ataccttctt ttgcacaata ataaagggca ttttgttcag cgtgaatggt
     2521 tctaatacaa tgtccatctc tcatttcatg accaacttca ctacaatgct tatctccaga
     2581 aatactacca ttatatccag tagacactat tctgttttct gaattgacaa ttatagctcc
     2641 tacataagct ctatcacaag ttcctctttc agctactgtt tcgcacagtc tcataaaata
     2701 ttcttgccat gagcatctat tttcaagttt tttattcaaa tcatatacct ccaaaattta
     2761 tatctaatat ataagattta taccttaaaa ataatattaa aaacaaattt atatgataaa
     2821 taaaatttat taatactgtt tttagtctaa attatttcat tttaaattaa agttacaagg
     2881 aatctaagat tatatatatc tttcttcgta atatgaatgt tacaatataa aaaactgtct
     2941 caataaaata gagacagttt tacagtcaaa gtttataata caagttcata tttttcttta
     3001 aattctttta cagcttcggg ccatgccttt tgttctgtaa tacctttttc tttagctatt
     3061 ttaattgcaa tctctcttat cattgcagcg ccttctaata ttcccatttt taatctccct
     3121 tcaaatatct ttttgtatag aatataaata gtgctaagat ttattacatg ctgcatatac
     3181 aatatctata caacacataa cttgtaaatt ccttttattt tcatacgtct ttaaccgaca
     3241 tataattata ttcaataagt tttaaaatta tgaactcaat tataattatt tttatttttt
     3301 tagaatctaa acactaaaaa ttgtaggtaa atttgtctta tgcctttata aatatttcta
     3361 cagttttaat tactggacat atctattttt attataaaaa gcgattaaaa atggagaata
     3421 tcctcataaa aactatatat aagcaaaacg gttaaaggaa aaagaagaaa tttacttgaa
     3481 tgttaaaatg cgttgcttga agcaatgggc agttttgcct ataatatcgg taacaactga
     3541 aagaaaggtg gttagcccta atgttaagcg aaaacattaa aacaatcagg aagtcaaaag
     3601 gactttcaca acaagaactt gctataaaac tgaatgtagt gcgacaaaca atatctaaat
     3661 gggaacaagg actgtcggtt cccgattctg atatgttgat ttccatatca gaggtgcttg
     3721 aaacgcccgt aagcacttta cttggagaaa ctattgttga gtcggaagtt gataacttaa
     3781 aagtgatttc tgaaaaatta gagattataa acttgcaact tgcacaaaga aaaaacatga
     3841 gaagaaaaat gtttcattgg ttatttattt tattatgtgc agtcatatta atagtttttg
     3901 tggtcttagg gagactaaat agtccttact tggattggaa ttataccgac cctgaaaccg
     3961 ctgtttttgg agtagctttt catgcttttg agtggctatt tgtcagatta gtaccgattg
     4021 tccttatagg agcaattatt ggaatctttt tgacacgtaa gaaaatatga agtaactctg
     4081 ctttttataa tacagcaagc gttccttaaa agcgtcattc tacatggcaa tcgaggacga
     4141 ctatgtaagg aagaaccttt ttgatttcca actaagcgaa gttattgagg acgacagtag
     4201 gcccaaaacc gcattaagta aagtacagga agcaagactg ctggacttat actctcatgc
     4261 gtcagatgca ggaaatgcga ggtctggtag catgatttac aacgatttgt acaacgacta
     4321 cgagtagaaa tatatggaga taagtattat ctacgaaatc agaaaagaaa tgacttgata
     4381 atttactcat gttataatta ttgatgtgaa attaataaat cgaaattcct tttttggatt
     4441 taacaatact gagtaaggaa aaagtgaatt acttttgcaa ggaggtgtta tttggatggt
     4501 gctagcaggt attttgattg tagtacaagg ctttttgact ggtcctttag caaaaaaaat
     4561 tggtgagaag cgactaatac tgataggtat gttttgcaaa gccttagtag cgtttgccat
     4621 ggtattgaca actgggtttg ttagtgtgtt ggtggttttt ggcttatttg cggtatcttc
     4681 tgctattaca gtacccacat taaacgcaac cctttcaaaa actgacaatc agcataaagg
     4741 aacactgatg ggatttgcca gtaccgccgg aaatcttagc aaagttattg ctcctttatt
     4801 aactgggtat ctgtatgaaa gcaatattga attaccatat ataaccacgg gagggatagc
     4861 cttaatcgga gtggttatct gctatatctg gataaaaaat caaaagaaca aatgatggaa
     4921 gaggacgttt atatggaggt tatacacagt aactaatctg gtatagaaat tcaattgagg
     4981 cttttctgct ttattaaagt cttgctacta cttaacccta taatatcctt aattcattat
     5041 gacaaattcc aattcaacag ttttgataca cataaggagg taacaatact atggaaaatt
     5101 atcaatgaag tgctaattct ttatcagtga agttatccat aagtttttct acctgtaatc
     5161 gtctggtgct ttttgccaga tcattggtag gtaagaaaaa ttcatcaaca gacacatgaa
     5221 gtaatgatac aaggtcactt gtatgcttgg atgttgccct ttattttcaa tattagttaa
     5281 gtaacgtggg tcaatatttg taattcgatt ttagggaaat atttaaagaa atattttcgt
     5341 tttatttctt taaatcttca agaatattga tagttttttg aaagtaggtg tgtattatga
     5401 agaatgagaa aaaaattaca gtcgcattgc tcattgttta tttattgatt ttatcatgga
     5461 ttattttatt taagctacaa ttttcatttt ctgcgttaga ccacataaga caaataaact
     5521 taattccttt tggtggttca gttattgcaa atggaaaaat tgacttcgat gaaattataa
     5581 acaatgccat tgtttttatc cctgtaggtg catatttcag tttgcttttt aaaaataaat
     5641 ctgttctaaa agcaataggt tctgttttag gaattagctt tgtatatgag ataattcaat
     5701 ttatatttgc tatcggagca agtgatatta cagacttaat tagcaacaca ttagggggta
     5761 taattggaat tgcattagta tatgtattat ctattgttct aaaggataaa acacataaga
     5821 tattaaatcg aatagcaata gtatgtactg tattagtagt tgccttctta tttatgcttc
     5881 ttgctgtaaa taatatattt taatgccatg ttaattacat gttgttttgg aaaagacttt
     5941 aatctgtcaa agtttgcttt tgaaattgaa catgaaatta caccaatgga acaaatgaaa
     6001 gctataaatt tttcattgga gaaagatgac tgtacaagta aagtattaat atacaatcca
     6061 gacaaatgga aatatgtaga atactatttc tataaagata gacctattaa aaaaactatt
     6121 ggaaaaattt attcaatttt gcatttgtca caataaaata tgttttgaaa tattgaatac
     6181 ttaaatatgc aaaaaaacaa atgtaactgg ctatggggct atatctacta aaaagctatg
     6241 tgataggttg ggattaaatt taaacatcac ttctagaagg catgaataca caaggctgac
     6301 aaactataca gttatgatta aacgatacca acgggattga aaatttcgtg aagaactttg
     6361 agaagtccta ttacacatga gcaacagcaa cgaagctatt gaagcaagga cgcaggcaat
     6421 ctagcggtta tctgacaaag gagttgtagg acttgaatgt agatactatc agaacggata
     6481 taccgaccag ctctgcggtt acagatgtga ttgtgtagaa tatcgagcag tcgggaacgg
     6541 acactttatc tgctacctac gaagtagatc agcagataaa agagagagaa cagacaacag
     6601 cggtcaaggc aacctatacc gtaaaagtcc atgtggacgc tgacggggat atggtaatcg
     6661 ttcagaacct tactcttgca ccagcaatcg aaaaatcaga ctatgagcca aagatatcag
     6721 aaacagacgc aagcgtggac gctgataccg ttaatgacgc taccgcattt ctggaaacat
     6781 tctttgaggt gtacccgaca gccacagaaa aagaacttgc ctattatgta tcggaaaatg
     6841 tgattgaacc tatcggcagg gactaccttt attctgaatt ggtaaaccca gtctttacaa
     6901 aggacgggga caatgtgaaa gtcaaggtag ctgtgaaatt ccttgataat cagacaaagg
     6961 caacgcaggt gtcgcagtat gatcttgtac tccaaaagga tagcaactgg aagattttag
     7021 gataacatgg aaatgggctt gtatcataat tatagtgtgc aagccctttt catattgaag
     7081 tatcaataat caataattat aattaggtac atgaatggag ggataaaatg ttcaaaattg
     7141 gtgagttttc aaaattgaca caagtttcta ctcgtatgtt gcgttattat gatgaaacgg
     7201 gtttattaaa acctgctaaa attgattcat taactggata tcgtatgtat tcagcagaac
     7261 aaatctcaac tttaaataga attatatatt tacgtgatag tggcttcaat gtcgctgaaa
     7321 ttgcattagc cctcaatttc agtaataatg ccattataga acagcttgat agaaagtcta
     7381 ttgaaattat ggataatata caaaaagaac aagaaaaact aaagaaaatt gagattgcta
     7441 aaaaagaagt gttacatggc aaaagtgaat tgtattttaa cataaccatt aaagagatac
     7501 cgagttattg cgttttatct ttaagaaaag aaataccaaa ttattattat gagggcgaac
     7561 tctggaaaga actctctgct tttgctaaag aaaataaaat aaatatttct gatgaaacat
     7621 tctcaatata tcacaatgaa gaatataaag aaaaagatgt tgatgtagaa ttatgtgcca
     7681 ttgtaaataa aataggaaaa gctacaggag attttaagtt tcgttttacc gaaccagttt
     7741 ctatgatggc ttgtactatg gtatatggag atttttctaa tatttcttat gcttatcgtt
     7801 catttgcaaa atggcttcaa gaaaacagtc aatataagat gaaaggttca agccgtcaaa
     7861 tagttcatcg tggaccgtgg aacgagaaaa atccagagaa ctatctaaca gaaatccaaa
     7921 ttcctttaga aaatctcttg taaaaaatga taatctgcct tgactttaac atggtgttaa
     7981 ggtttatcgt ttatttatca agaagaaaac ggaggaaaat ataatgacaa cttataaagt
     8041 aaattcaata ggtactatct gcaacaatga aaaaggtgct ttcattcaac tagaagcacc
     8101 atatattcta gcattaaggg cattagacgg attcagtcat ataaatgttt tatggtggtt
     8161 cagtgatttt gataatacga attttagaag tgtattggaa acagaacagc cttataaaaa
     8221 tgctccagag aaaatgggga tttttgcaac aaggtcgcca gtacgcccaa atccgattgc
     8281 tttatcagca gtagaaatta tcgatattga ttatatgagt gggctgatac aaattgctta
     8341 tattgacgct aatgacaaaa cgcctatctt ggatattaaa ccatacacgc cgagtttgga
     8401 tagagtggaa aatccgcaag ttccagattg gtgtagtcat tggcctcaca gcttagaaga
     8461 atctgcttct tttgcatggg aaagcgaatt caatttttaa ggagtgatga accatgagtt
     8521 tgcaggacta catttttgaa aaaccagtaa ttgaaacaga acatcttatt ttaagaacaa
     8581 tgaataaaaa tgacatagat gatttgaaag aatggattag tgatatttct ttatacgagt
     8641 attgggggaa acgtccctca aaaagcgaaa aaaatccaga attactcttt gataagtcaa
     8701 aaaaatctac taaaagtttt cattggggta ttgtccataa acaagatagc aaagtgattg
     8761 gtgaaatatg gatatatctt attgaaaata atagaatggc aaaagtggct ttcagacttt
     8821 ctaagcatta tcaaggtaat ggactaatga gtgaagtgtt gaaaaacgta attgaatttt
     8881 gtttttcaaa aacagaatta caaaaaatat ggacagatgt gcatatttta aatcatgctt
     8941 cttacaagac attggagaaa gtcggttttc aaagggaagg acatattcga ggggggaaaa
     9001 tggtaaatac ctattgcgac tactacctat atggaatttt aaaatctgat tatatcacta
     9061 ggtggtgcta tggctaatat tgaagcgacg attggcgaag caatgaacaa tacagttcca
     9121 aaggtctatg atacgaaaag cattaaaaga tatgtgatga atttattaga tatggctcat
     9181 taaaaagtac caaagaaata aaagttacca tatagacaaa attggttttt tatatctttt
     9241 gatttcttat aagataatgg cattgtgcga caagctatgc aaaaaatttt catgtatact
     9301 ttgattataa tttatacaag gaggtactga tatgtacgca tatgaaaata ttgaaatgtc
     9361 attaaactat attgaacaac atttaagtga gaaaatcgaa acagagaaac tggcggaaat
     9421 tgcctgtctt tctacatttt attaccaacg cttgtttaaa aggttagtta agaaatcagt
     9481 tcaagagtat atcaaacttc gccgtttagc aatggtaata gcagaattga ataattcaga
     9541 ggaaagaata ttagacatag ctttaaaata tggattttca gaccatgcta acttcacaag
     9601 agcatttaaa gaagtttacc atattactcc cgatgaatac agaaagaatg ttcctatgtt
     9661 aaatacattt gacaagccag aactttcttc acgttatatc atgattgatg aaaatgtccc
     9721 attgattgta ggaaatattg ttttggaaat tcaaagaaaa acattaagaa caaaggaaac
     9781 atattttggt tttgaaaaaa cagtaaatat ttctgaacaa attcctgctg gagaaagtac
     9841 aggaattgat gttccaggtc agctatggcg cgaattccat gcaaagaaac tgcaaaccga
     9901 aaatgccatt tttaacaatg atattgaact tggtgtatct cacttagcaa gtccagataa
     9961 aggaacattc ctttactttg caggaggtat ggtttcatca acagatgtat gtcctaaaaa
    10021 tatggtacaa catacattac cagcaggtga gtatattgta tgcaaaattg aggcagaatc
    10081 ttttgaagaa cttgttacta ctgctttaaa tcaagcaaat aagtatctgt ttgaaacttg
    10141 gcttccaaat cataatttgg ttacaaagcc attcatggca gaaaaatatt acaaaggaaa
    10201 tgcagagatt aattatatgg aaatatggat aattcctgtg taaacagtaa atgctaaata
    10261 ggaattatct gacgtaataa aaaggaaata gaaagcttat aaaggagggc atagtccgtt
    10321 gtgataaaca taaaagatat aaattatgta cctaatatta gtgaaataag cgtttatatt
    10381 gaaaactctg catttgatga gttgtatcag catatgaaca atgaatataa agcagtttgc
    10441 aaaatcgaat acagtaaaga tgtctgggca cgaggttgga acgtaaagtt taaaaaatct
    10501 gggaaatcat tgtgtgttgt ctatccgaag aagaactact ttactgtatt ggtggttgta
    10561 ggtaataaag agaaagaaga tgttgaaaac ttgttaccta ggctttctga atatgtacag
    10621 gaaatttatc gtaacactag agagggaaat ggacaaaggt ggcttatgat agacttaaaa
    10681 tctaaaaata atgtatatca agatgtatta aggcttattg aaattcgtta tgccataaaa
    10741 tgtgtaaaac cgtaataaaa atatttttca catacgtaat ctcaaggtat tatcgttgag
    10801 acaagtaacc tatataatta tgggaaatga gatttttaga tacttaagaa agtactttag
    10861 ggggcttgcg ttatggaaag atttaagtac aattccacac aatcaaatac aatctaataa
    10921 aactttacta aaaattgaat gcaatacagc tttaaaggct cgtacaaact atatgttttg
    10981 tgcgggcttt tttcatttaa taggaatttg gattcgtcag agagtatatt ataaaaataa
    11041 aacgataagg gtaccttaaa aataaggtac ctcaggctgt agacaaagtc ccggactctc
    11101 gtctgggctt ttcttatatt tgagttatcc acaattcaac tttttaagag ttatcttatt
    11161 aagaaaaaag tgtatgtttt ccaccctata gttttacttc tgtgatattt gcagatgcca
    11221 ctcaagtaga aagtggtggt acggcagaag atgtcatgca gagttcagaa agtctgggac
    11281 taccgcctaa ttccttagac acggaaagct caatcaagca ggggtgtaag tattttgcgt
    11341 ccctgctttc ttcctgcaaa aatcaaggta tcgacgattt gaatgtagcg atacaatctt
    11401 acaactatgg cgggggttat gtgggctatg tggcaggaaa agggaaaaag cacactttta
    11461 atcttgcaga gagcttcgcc cgtgagaaat cgggtggaaa gaaagtaacc tacgccaacc
    11521 cgatagccgt tgcaaagaac gggggctggc ggtatggcta tggaaatatg ttttatgtgg
    11581 aattagtcaa tcagtattta actgttccgc aggtatcggg agagctggca caaaaggtaa
    11641 tgaatgaagc gttgaagtat caagggtgga aatatgtgtt tgggggaagc aatcccaaca
    11701 cttcctttga ttgtagcgga cttgtgcaat ggtgctatgg aaaagctggt atctacttac
    11761 cgagaacggc acaaacacag tatgacgcaa cccagcatat ccctttatcg caggcaaaag
    11821 ctggggagtt gttctttttc cattcaacct ataacgctgg ttcgtatgta ac
//
LOCUS       DACULQ010000121        11810 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.122, whole genome shotgun sequence.
ACCESSION   DACULQ010000121 DACULQ010000000
VERSION     DACULQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.122"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            166..3264
                     /locus_tag="JA154_13250"
     CDS             166..3264
                     /locus_tag="JA154_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HAT7996811.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSSDFEKLLY
                     QK"
     gene            3387..4673
                     /locus_tag="JA154_13255"
     CDS             3387..4673
                     /locus_tag="JA154_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7996812.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(5004..6110)
                     /locus_tag="JA154_13260"
     CDS             complement(5004..6110)
                     /locus_tag="JA154_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HAT7996813.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(6145..7686)
                     /locus_tag="JA154_13265"
     CDS             complement(6145..7686)
                     /locus_tag="JA154_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HAT7996814.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(8048..8794)
                     /locus_tag="JA154_13270"
     CDS             complement(8048..8794)
                     /locus_tag="JA154_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT7996815.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(9006..10316)
                     /locus_tag="JA154_13275"
     CDS             complement(9006..10316)
                     /locus_tag="JA154_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7996816.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(10329..10799)
                     /locus_tag="JA154_13280"
     CDS             complement(10329..10799)
                     /locus_tag="JA154_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996817.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(10803..11654)
                     /locus_tag="JA154_13285"
     CDS             complement(10803..11654)
                     /locus_tag="JA154_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HAT7996818.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
BASE COUNT         4327 a         1651 c         1789 g         4043 t
ORIGIN      
        1 attgtacgat tgacacaata ataattctat ttactaaaaa atagaacctc attaaggtac
       61 tattttaaaa cataaagaag tataatatca ttgccaaata tatagcaaaa ttttaaaagc
      121 tttaaaaatt caatctggaa aatgaataaa aggagatata tataaatgga tgtaataaat
      181 ttaatagatg agttggatga aattgtatat gtttcttctt tagatacata tgaactttta
      241 tatattaatg aaattgggaa aaaggcaatg ggacttaatg atatatccca tttaaaatgt
      301 tataaagcat tacaggggaa agattctccc tgcgaatttt gtacaaactc tattttaaaa
      361 gaagatgaat attacatatg ggaaaatacg aatactcgaa caaatagaca ttttatatta
      421 aaggacaagt taattaaatg ggagggcaaa catgcacgat tagaaatagc tcttgatatt
      481 acagaaaagg aagatataag taagtcaata gcagaaaaat tagagataaa ggagttatta
      541 gttggatgta ttaaacatct tataacagaa tctaattttt ctgatgctgt aaatcttgta
      601 ttaaccaata ttggtaaatt tcataaagct gatagagcat atgtttttga agtatcagaa
      661 gatagaacaa aagtaactaa tacatatgaa tggtgcaatg aaggtgttga tgaacaaaaa
      721 caggcactaa caaatcttga tattaatgaa ttaaagaatt ggtttttatt attcgaacaa
      781 aaaggttttg taataattga tgatatagaa aatataaaag aagaacagcc aatagaatat
      841 gaaattttaa aacctcaaaa aattgaaagt ttaatgacat cttcactaaa acaagatgat
      901 aaagtagtag ggtttattgg agttgataat ccacataaag ctgttggaga tacctctctt
      961 ttgacatccc tatcttattt tttattaact gagatgaaaa aaagaaaagt agaaaataaa
     1021 ttaaattata tgagctacta tgacttcttg actggtgcat ataatagaaa taaatatatt
     1081 aagtatatag ataattttca aaaaggaaaa gttaaatcca taggagttgt atttgtagat
     1141 ataaatgggt taaaagatat taatgataac tttggacatg ctaatggcga ttctgctatt
     1201 gtgtcagcat gtgtaataat aaaaaaatac tttcattctt ataatgttta ccgtattgga
     1261 ggagatgagt ttgttgtatt atgtgaagat atatcaaaac aactatttga agaattagtg
     1321 agttggttaa gtgaagaatt taaggatgag atgatatata gtgtagcagt gggcagtatt
     1381 tggagtgacg ataatattga tatacataag ctaattaaag ttgctgatga gagaatgtac
     1441 caagataaga agaggtatta taataagaac aaacttagaa ataatgaaat gaaagtaatt
     1501 agtgacttag ctgattcgac gaataatatt ttatttaaac ataaagagtt agttgataag
     1561 gtttattttg atgatttaat gataaaaggg tattctaaag cttatgaaaa attagcttat
     1621 aatacatatg atataatttt atctatttgc attgatacga atttaattac tccaatttat
     1681 caatcagaga gcaatatttt tagttttcct ataaaggaaa atcttgaaaa aactttttca
     1741 tacataaaaa aatatttaat tcatcctgat gatataatat tatttaatga taaccacctt
     1801 ctaaaatctc ttgatagtct taataatagc aagcaagacg atttagaact aaattttgag
     1861 tatagacgtt taggaataga taaaaagtac tattgggcat taatttatat gttaaagatt
     1921 agtagtaaaa ataagttagg tgagaaacaa acacatattt tagttgctat aaaaaatata
     1981 gattatctta tgcaactaca taaaaaccaa aaagatacat taacaggact atacaattat
     2041 gaaagttttt gtgaacaaat aagtttaatg cttaaaagat atcctgacaa gcgttatgca
     2101 attataatga tggatataga taagttcaag gtaattaatg acatttatgg agttagagga
     2161 ggagataaag ctttaatata tgttgcagag ttgattcaaa aaaacattgg ttctaatggt
     2221 atatgttgtc gtatgtatgc agatgtattt tgcatttttt atgagagtta ctctgataga
     2281 gatattcata aagtaattta taagattaaa aatagcttaa atagaaaaaa atttgaatat
     2341 atgttagtac catgttttgg ggtttataga attttggata atcatattcc aattacaaat
     2401 gcgtgtgaaa tggcaggata tgctcacaaa aaagtaaaga aacaaagtat caacaactat
     2461 atattttatg atgatactat aagaaatgat gcacttgaag aaaaacagat ggaaagggaa
     2521 atggaatacg cacttgaaaa tgggcaattc catgtatatt tacaacctaa gtataatctt
     2581 aatgcaaata aaattgttgg tgcagaagca ttggttagat gggttcatcc agaaaaaggt
     2641 ataatacctc caattaaatt tatttcacta tttgagaaga atgggtttat tatcagatta
     2701 gatatgtatg tatgggaaca agtatgttct ttgattagaa aatggataga tttaggtgaa
     2761 gagccagttc ctatctctgt aaatgtatca agattacatt tgcataatcc acattttaaa
     2821 gaaattgttt taagtttaat aaataaatat agtataccaa aatctttttt agaattagag
     2881 cttacagaaa gtttatttat taaaaatata aaattattta ctaagataat agccgacctt
     2941 agaatggatg gtattgtttt aaatatggat gactttggtt cagcatattc ttcattaaat
     3001 atgttaaaaa atattaatat tgatacatta aagatagatt gtggtttctt taatgaggaa
     3061 ggaatcacag acagagaaaa aatagtttta aagcacacta tagccatggc aaaagattta
     3121 gatatggatg tagtggcaga aggtgtagag acaaaagaac aagctgaatt tttagtgagt
     3181 ttagattgtg tggtaattca aggttattat tattgtaagc ctattccaag ttctgatttt
     3241 gagaagttac tttatcaaaa ataagctaat tattccattc taaatgtagg taggatggag
     3301 tagttcaaaa taattaaata gaagtagaaa atagtgagga tataattttt aaaaagtata
     3361 atatttatgg attaggagcg aagtttatga tagtgggtat tataggtcct tcagactctg
     3421 ggtttaagat aaaggatgat ttaaaacaga tagacagtga tttaaagact aaaatatatg
     3481 taagagaaaa agtagtagat acaatagaag ttatttctaa atgtgaagac gaatgtgatg
     3541 ctattatatt tacaggatgt gcagtttatg aatttataaa atctaagtat gagataagta
     3601 agccacattc atttgtaccg agaagtggga cgagtataat gaaggcattt tgggcaataa
     3661 aaagtgcaaa tataaaatta gataaattca gtatagatgt agttgataga tatgttgtgg
     3721 aagatgcact taaagaattt gatattaaag caacgagtgt attttgtaat ccattttctt
     3781 tggaagtagg agagtctgaa cttgtagaat ggcatataaa attatttgaa gaaaataaga
     3841 cggatataat gttaactggt tttggtgcag tatataatga attaaaagaa agaggctatc
     3901 ctgtatttcg tttacaagct actatacaat taatcaaaga aagttatgac aaggtaaaga
     3961 gtgaatatgc tctaagtaag gcaagatttt ctcaaattgc tgttgaaata ttgagtttaa
     4021 tagattataa agagaagata gataactatt attctgacat gataaaaaaa tcagatatgg
     4081 ataagcttgt agtaaactat gtaagaagta ttcaaggttc cttattttta cttggaagaa
     4141 atgattatgt tgtatttgtt cataaagggg tagctgataa tgagtataat tatgataagc
     4201 tattcaatct aaagagagag ataaaagaca tgggcttttc tcttagtata ggaattggta
     4261 ctggagtaac tgcgtatcag gctgaaaata atgcacataa agcattaaga cactccatag
     4321 attcaaaaga aattggtata tatttggtag atgaagatga aaacataaga ggacctttag
     4381 cttccgaaaa tgaattaaat tattctttga tgctatctga tgaaaaggta atcgaaatat
     4441 ctaaaaagac tggaatgagt tgtgagtcag ttgcaaaaat tattgctata aatgagaata
     4501 gaaaaagtaa gatttatgat tcaaaagagt tagcagagta tctaaatgta agtgaaagaa
     4561 gtgcaagacg tatattaaat aaaatagtaa atgcaggttt aggtcgaata tgtgcaaaag
     4621 agactagtat tggtggagga agaccaaaaa atattactga aatactgttt taagatattt
     4681 tataaaactg tataaatcat tatattgtat aaaattctac aacaaattac tatataaagt
     4741 attaaagctt attatgaaaa aaattagaac aaatgattat gtaaaatact agaataaatt
     4801 attaatataa aatactaaag cttgttatta aaaatgctag aacaaattat tatgcaaaat
     4861 attgaagctt attatgaaaa atgttaaaac aaattattat ataaaatact aaaacctatg
     4921 taaaacaact ttaatatata acttataaag ttgaagatac ataggttttt atttttataa
     4981 tttaaagagt tatattatga tatttataat ttaatcatat gataaactat tttggcagta
     5041 tcatataatt cgtctatatt tacatattca ttaactgtgt gtacatcata catacctaga
     5101 cttaatatag cacaattata tccaagatta gctaatatat ttgcgtcact tccaccacca
     5161 attaccattg gatttggaac aacaccaact tgtcttattg cttcttcact taacttgaaa
     5221 acatgactat ctctactaag ttcaaaacta gggtaagaca tactatgttc aaaagtataa
     5281 gtagtattaa atttagatgc agcatcctta caacattttt ccatatgatt aagctcatac
     5341 tctaaagttt caggtatatg agacctaatt tcagcagtca atgtaacctt atcagttact
     5401 atgtttgtag ctccaccacc ttctatttta cctatatttg aagtagtcaa tgaatctatt
     5461 ctgcctatgt gcatgttgct tattgcatga gaggctacta atatagcatt taaccctttt
     5521 tcaggttcta taccagcatg tgctttcttt ccatgaaaag agatttttat agattgttga
     5581 gcaggagctt tgtatgctat cgaaccaata gctccaccag aatctaaaat caccatgtct
     5641 ttacaaggca agttgtcagg attaaagttt ttgacaccat gcataccggc ttcctcacag
     5701 attgtaaaac aaagataaat atctctatgt ggtatttttt ctgtaataac atgttctaaa
     5761 gcttcaagta tagcagctat tccagcttta tcatctgcac caagtgtagt agttccatca
     5821 cttctaacta cattaccatc tagtattggt tttacaccta aacaaggttg aacttggtcc
     5881 atgtgtgcac ataaacaaag tggtctactt ccttcttcac cttttatata ggcaatgaca
     5941 ttacctgtat taccaccata tttttcacct gcatcatcta taatggcgtc tatatttctt
     6001 tcttttaagt agtttaccaa ccacttagac atttctaatt cttgattaga tgggctatca
     6061 attctaacca tatccataaa attatttata agtctatttt tatctaacat aaattatttc
     6121 tccttttaga aagatttaaa taaattacat gctatatgaa ggaagtattc caggtcctag
     6181 tggaatatta aataccataa acaatattaa tagtatagtc cacactatta agaatgatat
     6241 agagtatggt atcatattag caattacagt acccatacca gcatctttat cgtattttct
     6301 tgtgaatgct agtataatag ggaagtacgg aaataatggg ctaagtggat ttgttataca
     6361 atcacctata cgataagcca tctgagtaag tgctggattg tatccaacca tcataaacat
     6421 tggtacaaat ataggtgcaa gaatagccca ctttgcagat gcactaccta taaataaatt
     6481 tataaaacat gataatataa taaatcctat caacaataaa ggtccattta ttcctgctga
     6541 ttttaaaaat tctccgccct ttacagctag tatcagacct aaattactat ttgtgaataa
     6601 ttgcaaaaat tgtgaagcag caaatgctag tattatatat ccacccatat cactcattgc
     6661 actagccatt atagagacta catccttatc cttctttatt ttacctatag tttttccgta
     6721 tacaacacca ggtatgaaaa atgctagtgc tattataggt accatacctt tcataagagg
     6781 agcattgttt gatagtaaat ctcctgtttc tgggtcagct aagaatggtt ttttaccgat
     6841 tacactaaga gctactataa tagccacata tattaatata cttataccag cttttttaag
     6901 ccctttcttt tctacatcac taagacttcc atcaacatct agttctgcat ttccttcata
     6961 tttaccaaat ctaggagcta caattttttc tgttacccat gttgccaaaa gtattagaac
     7021 aaaagttgat gctatcataa aaaataagtt cattgttgca ttggcttgaa atccagaatc
     7081 aatcatttga gctgctggaa ctgtaaaact tgcagcaagt atatcattga cacttatcat
     7141 aatgtttgcg gcaaatccta gacagactgc tgcatatcca gaaaataaac ctataagtgg
     7201 atgtctacca acaccaagat atacaagtgc agctaatggt ggaaataata ttgtacctgc
     7261 atctgaagct atattggcaa gcatacctat agtcattaca acaagagtta ctcttgtttc
     7321 aggaatcttg cttactgatg ctttgattgt tgctgtcata agtcctgttt tgtcacatac
     7381 accagcacct atcatagtta ctagaactag acccaaagga gcaaaccctt ggaaattgct
     7441 gactatgttt cctaaaagaa tttgaagctg ttctacagtc aataaattta cagcggttat
     7501 agtttctcca gtaccaggat gaactactga tatacccata gaaccaacta gactagatag
     7561 tataagtaaa agtacacata accctaaaaa tattgtaact gggtctggta gtttattacc
     7621 aacagtttct attctgttta atgtcctgtc aaaaaagttt ttcttcttaa ctacgttatc
     7681 attcataata tcctccctaa tgtttaaaaa tataattaaa aaataagtat tgtggttaat
     7741 cttacaattt aaattctgtt tgtcgattta agacgtgtcc acaatatgtc ctaaaaaaag
     7801 tataaaataa actttttcct gtgtcaatgc agttttctga attttctggt tgagtggtgt
     7861 tagttaaata tgctttaagt agattaatga cgaattttat ctaatgtatt taaataacat
     7921 atgcgcttta aaattaattt taaaacaaaa ttttatgaaa agctagtgga agattaagtt
     7981 attaaaataa gtagcgtagt aaagtgttag attttttaat taatcaaata aatttacttt
     8041 gtattttcta caaaaacctg tgaatatctt ttgaattatg tagaggatat aaatttattt
     8101 tatttacata gattagattt tgttctaatt tactgtattc aaatggtttt ccatgtccag
     8161 gataaatcat tttaggtttt gtattgatga ttgttttcca agattgaata aattctagtt
     8221 tgctttctgc aaatatggta attttgtgag tacttggaaa tccattcatt gcagaatctc
     8281 cacaaaataa aacaccttca tcaattagta gtgaaattga atcagcagta tgtcctggtg
     8341 tatctataat ttttccattt agttcttttt caatttcttt acgattcttg tcagacacaa
     8401 gaatacacct tttttcaaat tcttgtttaa gtggagaaaa actatgctca ccttttccaa
     8461 atagtttcat aatttggcaa aagagaaagg cagtacggct ggtacaacca ccaataaaag
     8521 agttttgacc tttatataga atttctaaag ctttatcatg cataacaatc tttgtattag
     8581 gagattcgtc tagaatctga tttagaaaac caacatggtc atcatgagca tgagttagaa
     8641 atatgtatcg aatatctttg atttttatgt tgaatttaga aattcgtttt ttgaaatgtt
     8701 taaaaccatt ttcatatcca gtatcaatca agacaaagcc atcttgaata gggtacaacc
     8761 atgtatttat aattcgattt cctgcattaa acattttttc accaccttga attagaatag
     8821 aaatttatat taattagaaa gaatatagca tagttctact gatatttcaa cgtagctcta
     8881 aaagttttaa ggaggataat atatcttagt ttaatctaaa aactaaaaga aaagaagtat
     8941 actgttacaa aaacaaagag tggtataact tttacagtag ataccaccct attataaaaa
     9001 tagacttata aagttttatc gtgtttttct aagaatgtta gatattcttc tttagtcata
     9061 actggagtaa aggtatctaa tatatcttta gcatccttag cattatcaaa aagaaggtct
     9121 acaactgtaa gtgccattgc tttagcagga attatgtaag ctaaatcttc atcaactatt
     9181 tcaaaatctc ttgtatgaag agctcctttt acaccaccaa tcatagggtg aagagtaggc
     9241 attatatgag acacatctcc aaaatcaaaa gagcctgtaa aatctccacc atcaataact
     9301 tttcctttta gacctaaatc ttcaagattg tttttaaata aattatcaag acttttgtat
     9361 cgtaatattg gaagatatcc aggaatttca gttatctcaa catcagctcc aactgccatt
     9421 ccaccagcca tcaaagcctt atttattctt tcattagcat caatcatacc atttatagtt
     9481 ctagctctaa catatgattc catatgaaca tcagcaggaa caacatttac tatatctcca
     9541 ccttttgtga tgatagggtg gaatctaact ctttcagact ctttaaaagt ttctctaagt
     9601 gcattcacgt tgtttatagc aagcattgct gcatttaaag catttattcc atcatgtgga
     9661 gcagaaccag catgagattc tttacctata aatttaacct tttttccaac aaatccatta
     9721 ctctcaggac caactagagc cttatcttct cctaaatcag aagcatgaaa catcatagat
     9781 atgtctatat catcaaaata acctcttttt attaattctt gttttccacc aaaataagtt
     9841 atttttcctt ctttttttaa ttgctctctg tattccaact caataaactc ttcagcagga
     9901 gtagccataa aagatacttt accatctaaa tgttctaaaa cgccactttt tactaaacca
     9961 acagcagcac ctaacattcc agctatttga atattatgtc cacaagtatg agaagcacct
    10021 atttcattag catctttatg ttctttacat gatataccat ccaattctcc aagtatagat
    10081 atatgaggac ctttcttgct agaatttgca tctgctttgc atcctgtaac tgctatattt
    10141 ttttctacag ataaatttaa ttcttgctca aaaaagtttg aaacagcttc tgtagtttta
    10201 aattctttat atccatattc tggatttttg tatattcctt gacctatttt taatattttt
    10261 tctctatttt catctatagt ttttattact agagatttta attgttcttt attcataata
    10321 aattcctcct atattttacc ttgaatactt aaaactatat gagctatact cgcacatgca
    10381 aagaatatag ttgtaaatac aataagtgaa attataacta ttttccatga catttctttt
    10441 aaagcttcta ttttgtttcc aacagaaatt cctgcaaaag ctagtagtgg agtagttata
    10501 gccataaagt taatattatt agtgttagtt aagaatactt cagatgttgg actatatggc
    10561 atactaagta aaaggccgat tatagttgca aatccaaaag ctggaaagat tgatttagga
    10621 aataaatctt ttataatcat agctgccata gcagctaata ttagcatcac cataccagga
    10681 agagctgtaa ctatagatgt accaacgcta attctttgtc ctataagaat tataactcca
    10741 ataacagaca taaccaataa ttgatatgaa cttttcttta aatttaaatt agcaaacatt
    10801 tattagcact ctccttctgt agatttaatt tctttagttt ttttatttct cgaaaattta
    10861 ggttctaaaa tcttatacat aaaattagta aatggtaagg atacaaaaac taagaatata
    10921 attcctgtta tactagacat catattgctt gttgcagcat aggcaagtat tgtatctgcc
    10981 atttcaggtg gtaccatggt agataatgaa ccagcagctg ctgtcatcat acttccacta
    11041 ccaacaccac aggccattcc caaagcatat gggtgaagac cactataaat agatattgaa
    11101 cctagtacac taaagtatat agtaccaaga actgttccca ttaaatatgt accaagtaca
    11161 ccactacctt ctggtgagtt tatcccatat ttttctgata taactcctaa agaaggttca
    11221 cgacttatac ttgcagtagc tccaacagct tctcttttca ttccaaacat taatgcaaga
    11281 ggtaatgcaa cgattggtgc aagtatatga ccaaattctt gagcgataaa tgctggtcca
    11341 gcttgaatta ttttgtcaat gtttggtcca actgtagtac catattttat accaagcatt
    11401 actagtgcaa ttccaacaac ttcaccagca atatttattt ctttttctcc aaccactttt
    11461 ttaagtgctt taattttttt acctaataaa tctggtgtta ttataagtcc aattataact
    11521 gcataaagca tagggaacag tactatagag gcttttccaa tagctatagt tctttgccct
    11581 ataaattcac atattactac caagattaag caagttaaaa atacttgaat aaaatgtttt
    11641 ttattaattt ccatgatttc ctccccaaaa gaataaatat tttacttatc tgaaatgaga
    11701 aaatacgttt tcttaggtat aatacgtttt cctaaataaa atgaaaaaac tatttcaaat
    11761 aagtttcaga aatgtcaata acatgtcctt aattaaggat aaaacaaaaa
//
LOCUS       DACULQ010000122        11561 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.123, whole genome shotgun sequence.
ACCESSION   DACULQ010000122 DACULQ010000000
VERSION     DACULQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.123"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..432
                     /locus_tag="JA154_13290"
     CDS             <1..432
                     /locus_tag="JA154_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996819.1"
                     /translation="EDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNKIMVKLKISNQ
                     IVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSVKFVILEGITY
                     ENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            433..1146
                     /locus_tag="JA154_13295"
     CDS             433..1146
                     /locus_tag="JA154_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAT7996820.1"
                     /translation="MIIMGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVD
                     LVNEAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSL
                     DRIALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKI
                     KGLMTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEG
                     ATIVRVGTSIFGERNYNKK"
     gene            1186..1644
                     /locus_tag="JA154_13300"
     CDS             1186..1644
                     /locus_tag="JA154_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAT7996821.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            1663..1920
                     /locus_tag="JA154_13305"
     CDS             1663..1920
                     /locus_tag="JA154_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAT7996822.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            1930..2715
                     /locus_tag="JA154_13310"
     CDS             1930..2715
                     /locus_tag="JA154_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAT7996823.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            2750..3277
                     /locus_tag="JA154_13315"
     CDS             2750..3277
                     /locus_tag="JA154_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAT7996824.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     misc_binding    3452..3684
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3777..6884
                     /locus_tag="JA154_13320"
     CDS             3777..6884
                     /locus_tag="JA154_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAT7996825.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     gene            7171..8043
                     /locus_tag="JA154_13325"
     CDS             7171..8043
                     /locus_tag="JA154_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAT7996826.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            8170..8442
                     /locus_tag="JA154_13330"
     CDS             8170..8442
                     /locus_tag="JA154_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996827.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(8605..9177)
                     /locus_tag="JA154_13335"
     CDS             complement(8605..9177)
                     /locus_tag="JA154_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7996828.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            9361..10203
                     /locus_tag="JA154_13340"
     CDS             9361..10203
                     /locus_tag="JA154_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAT7996829.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEVGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(10320..11396)
                     /locus_tag="JA154_13345"
     CDS             complement(10320..11396)
                     /locus_tag="JA154_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAT7996830.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
BASE COUNT         4521 a         1301 c         2016 g         3723 t
ORIGIN      
        1 gaagattcta ataactttga aaaaaatcaa aatgtaataa ttgaatatca tgagcaaaaa
       61 ttagatgcaa aagtttacaa aatatatgaa aataataata aaataatggt aaaattaaaa
      121 ataagtaacc aaattgttgc aaatcaggat acaagtgtcg aagaatttga tataatatat
      181 aatcagatgg aatgtttaaa aatacctaaa acttctataa aaacaaaaga caataaacgt
      241 ggtgtatatg taattgatga acaatcacaa agtgtaaaat ttgtcatatt agaaggaata
      301 acttatgaga atgaatcttc tgtatttgtg gattattata aaaatgacat aaatggagtt
      361 acaagtgtta atctatatga caaaataatt ttaagaccta acagtataaa cacaaatata
      421 aggataaagt aggtgataat aatgggttct attaaagaca atttgtctga gataagaaac
      481 aaaattgata aagctgcaaa aaaagtagat agagacagca aagagattac ccttttggca
      541 gtcacaaaga ctgttgatgt agacttagtt aatgaggcaa tgagggaagg aatcacttct
      601 gttggtgaaa ataaacccca agaattagct agaaagtatg atattatagg tgataaatta
      661 aactggcacc tgataggaac tttgcaaacc aataaagtta agtacataat cgataaagtt
      721 tgtatgatac actctttaga tagaattgca ttatgcgaag aaattcaaaa acgtgcagaa
      781 caaataaaca gagaaataga ttgcttagta caagtgaata tatctaaaga agagtctaag
      841 catggacttg atagagaaga agttgtagac tttgtcaaag aagtgtcaga aaaatataaa
      901 aatataaaaa taaagggtct aatgacaatg gcacctttta tagaagatga agatgagata
      961 agaggtgtat ttaaaggtct taaagattta tcaaaacaaa taaaagaact aaacatccct
     1021 aatgtaggga tggatgtttt gtcaatggga atgagtcacg attatgaaat tgctatagag
     1081 gaaggggcta ccattgttag agttggaaca tcaatctttg gagaaagaaa ctacaataaa
     1141 aaataattac tcaaatttga ataaatttta aggaggaagt taataatgtc aaatggaata
     1201 atatctaaat ttaagaattg gatagttgat gaagaagaag attacattga agatgaatat
     1261 gaaagtggca tggacgatat agttcaagaa gaagaaatga acagtggctt tagtactgca
     1321 aaagcaaata aaattgtaaa tttacataca actagccaaa tgaaagttgt aatagttgaa
     1381 cctaaggttt atgatgaagc tgcaactata gcagaccatc taaaacaaag aagggcagtt
     1441 atagtgaatt tggaaggatt gactaattct gaagttcgta aatctatatt taactttatg
     1501 aatggggcag tttatgtctt agatggaagt atacaaaagg tttctaaatc aatatttata
     1561 ctagctccta ataatgtgga tatagatgca aatatgaaaa aagaattaga aagtaaagca
     1621 ttcttccctt ggcaaaataa atagtaaaaa ggcggttaat atatgggaac aataagaatt
     1681 gcactttatt atcttttaga tataatagca tggatgatag ttataaaaag cttgatgact
     1741 tggtttccaa atggaagaca aagtaaaatt tttgatattt tggaaaatct tacagagcca
     1801 atagaagggc caataaggtc tataatgtat aaatatacaa atggacctat agatttttct
     1861 cctttaattg caatagtatt attgatgttc ctaagacaat tagtattggt tatattctaa
     1921 ggtgatagga tggataaatt aaaactaaca aatcacataa aggatataga cttgaaaaat
     1981 aaaatgttta aagtgataga caaggctaat agttgtataa aaaactatga tgttaagtct
     2041 acggagtttc ttaatccata tgaagtgaaa aatgctgtag caatattaaa ttctacaaat
     2101 gaaataaaat acagtgtgga tggaggttat gaacaagctg aaagaagcac tgtatttatt
     2161 tatccatttt acatggaata tgaagatata gaagatactc ttagattttt acaaatagaa
     2221 gggaatttca aatttaaaaa tatatctcat aaagattact tagggtcaat actgggtcta
     2281 ggtataaaaa gagaaaaaat tggagatata attatacatg atagcttttg tcaagtagtt
     2341 gtaagtagtg atatttgcga ttttataatt gtaaacttag aaaaggtttc aagaaataat
     2401 gtaattgtaa aggaaatttc cagagaaaat attgttaata gttcttcaaa atataaagaa
     2461 gtatcattta cagtatcttc agataggtta gattgtgtga taagtggtat ttacaatata
     2521 tctagacaag atagtgcaaa atacataaat ggagaaaaag tccatgtcaa ttatgaaaag
     2581 ataacttcaa cttctaaaat agtaaaaaat gatgatttga tttctattag aggcaaagga
     2641 agagctaagg taacacaaat aggagatatt actaaaaaag gaaaaataaa agttcaagca
     2701 agattaattg tataaggact aatttaaatt atagctggga ggaaatgtaa tgctaactcc
     2761 aattgagata gaaaataaag agtttaaaaa aggtttaaga gggtatagag atgaagaggt
     2821 tgatgaattt ttagatatca tcaaggaaga ttatgaatct ctatgtaggg aaaatacagc
     2881 tcttaaagaa aaattagggt tatatcaaga ccaagtaaac aagtatgaaa atattgaaga
     2941 aacactaaaa gctactttaa taactgctca aagtgcagca gaagacactt gtagcgcggc
     3001 taataaaaag gctaagataa ttgttgaaga agctgattta aaagctagac aaattataga
     3061 acaagctaat aaccgtgtta tagagataag aaaagagtat gatgctttag tcaaagaatt
     3121 taagatattt agaaataaat ttaaatcact gttagaagat gaaataagaa gtgttgatga
     3181 gattttttat gatgtagatg aaaaatgtgt aaacgcattt gaaggaacaa cagtgtataa
     3241 ttatagtaat gatgaagctg ctacaacttt agaataagct tgtaatttgt ttatttttta
     3301 tgtagaataa ttgtgtgagt ttgagaaatc tataacaaag attaaaaata ggataaaaat
     3361 gtaagaaaaa tagaaattat tatatttttc aaaaatatat tgactattca aattatttgt
     3421 tatagaattt catataaaca cagataaatt tttaaaagcg ttgatagaga catttatctt
     3481 tagataattt tacagagaat tgggtatgct gagaaccaat gaatttataa agataaaaat
     3541 cactcttgag ctgcaggctg aacgattaaa ttctactaag ctgtgccggt tagtaccgtt
     3601 atactattag agtgttatat gtagctagta tttagtttac atatagaatt agggtggtaa
     3661 cgcgatttat aactcgtccc tttggtttaa agggggcgag ttttttagta tttaaattta
     3721 tgtgaaagaa taaaaaattt tggaattata atatttagta ggaaagggtg atttacatgg
     3781 cgaaatttaa gccattggta gacagttctg taaaacaagc agaagcacaa gtttttgatt
     3841 attggaaaga cataaatata cttgaaaaaa ctttagaaaa agggaaagat gacccaagtt
     3901 tcgtattcta tgaaggacct ccaacagcta atggaaatcc tggagtacac catgttttat
     3961 ctagaacgct taaagattca gtttgtagat ataaaacaat gtctggttat caagtaaaga
     4021 gaaaagctgg ttgggataca catggattac cagttgaaat acaagtagag aaagagttag
     4081 gacttactag taagcaacaa atagaagaat atggaatagc tgaatttaac caaaaatgta
     4141 gagagtcagt tttctcattt gaaaaacaat ggagaataat gactgagaga atggcttatg
     4201 aagttgattt agataatcca tatataactt tagataataa ctatatagag tctgtatggt
     4261 ggatactaaa caaattcaac aaagaaggat atatatatga agggcataaa atacttcctt
     4321 attgcccaag atgtggaact ggtcttgcta gtcatgaggt tgcacaagga tataaagaag
     4381 taaaaactaa tacagttata gctaaattta agaaaaaaga tgctgatgaa tacttcttag
     4441 catggacaac aactccatgg actttaccat caaatgtttc attaacagta aatgctgatg
     4501 tagattattt aaaagttaaa aaaggtgatg aggtatatta tgtatctaaa cctttagctg
     4561 ataaagtact aggtgaagat tatgaagttt tagaagaaat gaaaggtaaa gacctagaag
     4621 gtttagaata tgaacaatta atgccttttg tagaagttga caaaaaagca ttttttatta
     4681 cagtaggaga ttatgtaact actgaagatg gaactggtat agttcataca gcaccagcat
     4741 ttggtgaaga tgactacaac acaggtaaaa gatatgattt accagttatt caaccagtag
     4801 atgaaactgg taaatttaca tctactcctt gggaaggtag atttgtaatg gaagatgggc
     4861 tagatgttga aataataaaa tggctagctt ctgaaaataa attatatagc aaagaaaaag
     4921 tagttcataa ttatccacac tgctggagat gtcaaactcc attagtatac tatgcaaaac
     4981 caagctggta tatagagatg actaaactta aagataaatt aatagaaaat aataatggtg
     5041 taaaatggtt cccaagtttt gtaggagaga agagatttgg aaactggtta gaaaatctaa
     5101 atgactgggc aatatcaaga actagatatt ggggaactcc acttcctata tggagatgtg
     5161 aatgtggtca tacagattca gttggttcaa gagctgaatt agctgaaaaa gctatagaag
     5221 atgtaaatcc agaaacagtt gaacttcata gaccatatgt agatgatata cacttaaaat
     5281 gtgaaaaatg tggaaaacca atgacaagag taacagaggt tatagactgt tggtttgata
     5341 gtggggctat gccatttgct caacaccact atccatttga aaataaggaa aactttgacc
     5401 aattgttccc agctgactat atatcagaag gtatagacca gactagaggt tggttctact
     5461 cattacttgc agtatcaaca tttgtattag gaaaagcacc atataagagt gttttagttc
     5521 cagaccttgt acttgataaa gatggtaaga aaatgagtaa atcaagagga aatacagtaa
     5581 atccaatgga attatttgac caatatggag cagatgcatt aagatggtat ttactatatg
     5641 tttctccacc atggactcca actaaatttg atatggatgg attaaaagaa atccaaagta
     5701 aatttatagg tactatgaag aatgtatata acttctttac tttatatgca aacacagatg
     5761 atataaatcc aactgaattt tttgtagaat ataaagatag accagaactt gatagatgga
     5821 tattatctaa gttcaataat ttaatgaaag atgtagaaga aaatttagct atatttgagt
     5881 taaataaaac tttaagaatg atacaagatt ttataaatga tgatttatct aactggtata
     5941 taagacgttc aagaagaaga ttttgggcaa ctgaattaac agaagataaa aaagcagtgt
     6001 acaatacaac ttatgaattg ttacatggtc tttgtagagc tatagctcca tttgcaccat
     6061 atatgtcaga agaaatgtat agaaacttaa caggagaagt atctgttcat ttagctgaat
     6121 atcctaaatg taatgaagag ttagtagata ctaaattaga agaaaaaatg gatttagcta
     6181 aaaacttagt tactttaggt agagcttcaa gagaagtaga aagaataaaa gttcgtcaac
     6241 cattacaaaa agtgttagta gatggtaagt ttgaagatac aataagtgat gtagttgatt
     6301 taataaaaga agagcttaat gttaaagagg taatatttgc taaagattta gatgagtata
     6361 tgaattttag tttaaaacca aactttaagg aagcagggcc attattaggt tctaagatga
     6421 atttattcgt tggagcatta agtaaattaa atgctcatga aactgctaat aaacttgaaa
     6481 aaggtgagac tttaacagtt gatttagatg gtgaagcttt tgaatttaat aaggatttag
     6541 tattaatagg tataactgct aaggaaggat ttaatgtatc tgttgaaaac aatctttttg
     6601 ttatactaga tactaagcta actgaagagt tgattaatga aggttatgct agagaattta
     6661 tatctaaagt acagcaatta agaaaatcta ataactttga agtattagat aatatagtaa
     6721 tagaatactg tggtgatgat gaaattgcta aagctgttga ccattttaat gaatatatca
     6781 agtctgaaac tttagcttta gaaataaaca gagtagatga taaatcttta gaggaacaaa
     6841 acttaaatga ccatatgaca ggtataaaag ttataaaaaa atagttagtt aatcaaaatc
     6901 cctatagaaa ttagtttata gatttctata gggatttttt atgtaatata attatctatt
     6961 caataaaatg ttttaatatt ccaaaattta caataaataa atctgtataa atcatataaa
     7021 ttatttatgt catgtgatag aatattaatg aataaacgtt taagtgagga tgaataatat
     7081 ttttaggaaa ctacttctga taaatttatt tgtaaattta tcgtgtgtta tgcagggtgg
     7141 tttatttatt atgcaaaggg gtgcgttttg atgtatggtg aaagtaaaaa gctaagtatt
     7201 gataataata tatttttaca atattgtctg attatattgg gtatgattct atcaacctta
     7261 ggaataaatt tatatctagc accagccaag ttattaagtg gaggagtagc tggaatttgt
     7321 gttatattat acaaattatt tggtataaat cagggaatat caagtttctt gatgaacata
     7381 ccaatattta ttatagctag aaagtatttt gataataaat ttttgctaat aagttttgtc
     7441 aatatgttac tattttctct agcactgggg ctaacacaag atattgctaa atatttccca
     7501 atagatgata ctatgctaca gtgtatttat ggtggtgcat taactggtat aggtatgggg
     7561 ttaactttta aagcaagagc tactgctgga ggacttgata taatagcagc cataatgaaa
     7621 agaaagtatg atatacctat gaaaaataca tttttattta ttaatttttt tgtagtatgt
     7681 gcaggagctt ttttatttgg agctaaattg gtgatgtaca ctttaattac aatgtatata
     7741 atttctttta ctatggatat aggaaaagat tgctttgata gaaaaaaatc tatactttta
     7801 atatcaaata aatatgatga aatatccaaa gtgataatga ataaaatggg tagaggagtc
     7861 acttttttgg aggcagaagg tgcatataca caaaataaga aaaagatgat ttattgcata
     7921 atatctgcaa atgaaatagg taaatttaaa gatttaattt atgatatgga tagtgaagct
     7981 tttatttctg tcaataacgt tgaagaagta aaaggtggag gttttaagga taaatttctt
     8041 taagattata tatgttaatt atatatgtta aaaaatgaca tatgtattga gattatacct
     8101 agttagatgt ataccatagt taatggggta catgaagtac tcaataatat actaggaggt
     8161 ataagaatta tgttaagaga gagatttgta gtaagaaatc atcataatcc acatacaagt
     8221 gggtgttgtg ggactggagg agaaggatgc tgtggaggag ataaccatga acattcacat
     8281 caacattctc acagtcatgg gcatgaacat gagcatgtaa atggtgtagg tcacgaccat
     8341 aatcatgatg atggtttttc aggtactcat gagcatagcc atgagcatac acatacacat
     8401 ggtcatgaac attcagatgg gcatacacat gaacataaat aaaaacattt taaatttata
     8461 agagattttt attttgtgat tccatgaagg tttaaataaa aattgaatgt tttaagtaaa
     8521 aattattgag aataaataag aaagtgaact gttaaagtag ataacttaaa tagttcactt
     8581 ttattaatta tctaagtatt aagtttaaat ccaatgatta actaaaaaag aagctatatt
     8641 ttcataaggt attttttctg tttttaataa actttggaca actaaaccat caactattga
     8701 catgagtaaa aaagaaaatg attcagcatc ctgttcattt tcgctactat tatatttttc
     8761 tagtgattca acaatttcag atttaagagt agttctccat tcaatatatt ttaatcttat
     8821 tctttccttt aaatcattat ttgaagtaat tgcctcacaa ataagataca tatgtaccct
     8881 accacgactt tctatagtag atattttctc taagattaag ttaaccattt ctatttgaga
     8941 attttttgat ttgacatttt ttacacagtt tagtatagca ctagttataa cttctaaatg
     9001 agtatccgca atatcatata taatatcatc tttagatgcg tagtgataat acaaagtacc
     9061 tttacttata tttacatctt ttgcaatatc agcaaggcta gtattttgaa ctccattttt
     9121 ggctattaaa ttggttgcag atgataaaat agcctgtttc atattatttg ttttcataaa
     9181 acctaccttt ccatgtatat ttataacaaa ttaataacat tataagctta tggatattta
     9241 ttgtcaataa agcgagaatc tatatctatt gacttttttt cattcaatat ataaaataat
     9301 aattaaatcg gtcgtacgac cgactgaata aataaaataa tatcttatag tgaggttaat
     9361 atggataaaa taaaattgat agtggacagt gcatgtgact tacctgatga tataatagaa
     9421 aaatacaaca tagaagttgt tggtttaaat gtatcttttg gagaagaaag ttatatatct
     9481 ggaaaggaaa tagataatga aactttctat agaaaaatga gtgagagtaa agtactacca
     9541 aaaacatcat gtccttctcc tgataaattt ttagaagcat accattgtca ggaagaaagt
     9601 gtattagtga ttaatataag ttcaggatta tctgggactt ataatagtgc acttttagca
     9661 aaagatatgt ttgaaaagga aggaaataac aagaaagttg aggttataga ttcacttagt
     9721 gggtcaattg gaataggaca attagtgtta aaagcagcaa tattaataaa agaaggaaaa
     9781 gaaattgaag agattgtttc aataataaat aaatataaag ataatcctcc attttttgga
     9841 acacttgaaa ctttagaaaa tgctattaag ggtggtagaa taaatccaat agcaggaaaa
     9901 attataaata ccttaaattt taaggcgata atacaagttg cagatggcgt tgtaactcct
     9961 atagataagg caagaggtga aggaaacagt ttgaaaaagc tgattacttt agtagagtca
    10021 aaaattaaag ataaagaaga aaaggtttta tttataggac atgctaattg cccagaaaag
    10081 gctcaaaagg taagggaagt tatggaaaag gatgtaaaat acaaagatgt tgtaatctgt
    10141 gaagttggtt cagtgatggg gacatatact tcaaagggtg caatcttaat aactgcaata
    10201 taaatactgc aatataaata atggattgaa ataaatcata gattgagaaa ttaaagccat
    10261 ataaaaatgt ttaaaacatt tttatatggc tttttaattt tatcaaattg attttagaat
    10321 taaccaacta cacatatttc tttgtcatgt ttgtttaata tctcacaacc atcttcagta
    10381 acaagtacta aatcttctat tctaactccg aactcacctg gtaaatatat acctggctct
    10441 atagagaata tcattcctgg ttgtacagta tctgtattta tagaagatac atctccttta
    10501 tcatgaactt caagtcctat agagtgacca gttctatgag tgaagtactg tccatatccc
    10561 ttttcagtta tgtagtcacg agcagcagca tctatatcac agaatctaac acctggtttt
    10621 acaatagctt cagctctctt attagcttca agaactattt caaatatttc tctacctttt
    10681 tcaggtattt ctttatagaa tacagttctt gtcatatcag cacaatagtt atcttttata
    10741 caacctatat ctaagattat tgcatctcct ggtttcacaa gtgcatttcc aggttcacca
    10801 tgtggattag ctccattagg tccaaaacct ataatagggt caaatgaaaa tccatcagta
    10861 cctaggtctt catatatttt agataattta ccaacaagtt gtttttctgt aagttcacca
    10921 gaaacagtag ctttaagttg ttccatagcc ttgtcattta attttgaagc tattctcatt
    10981 aactcttttt cttcttcatc tttacacatt cttaaagtat ctattatata agatgaattt
    11041 acaaacttac ttcctccacc taattctatt aagtttaata agaatctagc tggccagttt
    11101 ttatcaacac ccatagtagc atttttgtct atatgttcag ttattatttc tactggattt
    11161 tgagtatcat tgaaccaaac catttcaaca cctaaatctt ctgaaactgg gaataattca
    11221 tttataaata atttattatt gccatttaaa tttaagtata atgctaatag tctttcgcct
    11281 ggatggatta gtactcctgt aagataaaat atagaagtag ggtcacttac taacatttga
    11341 gaaatatcat cttttttcat ttgttcaagt acagcattta atctttgagc tttcataata
    11401 atttcctcct aatcaaaatt caatacaaat caattttagc attattaaac aaaaaagagt
    11461 atacttttct tctagaaata taaaaaaaat ttgttatata ttttttatat taagtttaat
    11521 ctctactttt gcattaaata cggaagtaaa atgacttttt a
//
LOCUS       DACULQ010000123        11505 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.124, whole genome shotgun sequence.
ACCESSION   DACULQ010000123 DACULQ010000000
VERSION     DACULQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.124"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            7..1467
                     /locus_tag="JA154_13350"
     CDS             7..1467
                     /locus_tag="JA154_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HAT7996831.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAYKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSA
                     RLKNVSKVYIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVN
                     TVMLTNAYKGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTL
                     VNNTKSTRLGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLV
                     NDGSNKSILKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGM
                     YKVGKDISAGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYI
                     KIEDSTMTLAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLG
                     NAEGIVTNDTFSGRRYITVEEDQYLILNDCYLIENE"
     gene            1715..3151
                     /locus_tag="JA154_13355"
     CDS             1715..3151
                     /locus_tag="JA154_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088491.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAT7996832.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(3558..3896)
                     /locus_tag="JA154_13360"
     CDS             complement(3558..3896)
                     /locus_tag="JA154_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996833.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(4194..4805)
                     /locus_tag="JA154_13365"
     CDS             complement(4194..4805)
                     /locus_tag="JA154_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme A pyrophosphatase"
                     /protein_id="HAT7996834.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            complement(5036..5785)
                     /locus_tag="JA154_13370"
     CDS             complement(5036..5785)
                     /locus_tag="JA154_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996835.1"
                     /translation="MAKNHQENYDVHIRFFTLTNEVPICGHATIAAHYVRAVENQFNN
                     KTVFQKTSAGILPVDIIKKDNDYEIIMTQGKIEVSPPLQQTYQKRLLNAFGILKEDIR
                     TDCPIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETL
                     QVHGRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPTPNTEFIFEAVQGETIKRKGK
                     MLVKVIILDNKPVEVKIIGKAVIAFSTNIEI"
     gene            6205..6561
                     /locus_tag="JA154_13375"
     CDS             6205..6561
                     /locus_tag="JA154_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7996836.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            complement(6676..6852)
                     /locus_tag="JA154_13380"
     CDS             complement(6676..6852)
                     /locus_tag="JA154_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996837.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     regulatory      complement(6905..6994)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      7614..7713
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7844..8134
                     /locus_tag="JA154_13385"
     CDS             7844..8134
                     /locus_tag="JA154_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HAT7996838.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     gene            8109..8879
                     /locus_tag="JA154_13390"
     CDS             8109..8879
                     /locus_tag="JA154_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996839.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSIHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            8901..9914
                     /locus_tag="JA154_13395"
     CDS             8901..9914
                     /locus_tag="JA154_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7996840.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            9880..10617
                     /locus_tag="JA154_13400"
     CDS             9880..10617
                     /locus_tag="JA154_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996841.1"
                     /translation="MMLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFV
                     SILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLI
                     LKKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDE
                     PFGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKE
                     FVIDSRKLNEDNLENIIKLKKEIISLL"
     gene            10778..>11505
                     /locus_tag="JA154_13405"
     CDS             10778..>11505
                     /locus_tag="JA154_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAT7996842.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYI"
BASE COUNT         4481 a         1287 c         1787 g         3950 t
ORIGIN      
        1 tttattatga aattctataa aagaatatta acattgactt taagtatttc ttttgtattt
       61 gcaaatattc aattagtaga tgcaatatca tctgtagaaa aaatacaagg aaaaaataag
      121 tatgaaatag caggtaaaat agcagataaa aatgcatata aaactgcata taaaactgca
      181 tataaaactg ctatattaat taatactagt aactctattg cagatggatt aagtgcaagt
      241 ggactagcag gagctttaaa tgcccctata ctattaactg aaaaaaatac tataccaact
      301 gagacatctg caagattaaa aaatgtatca aaagtttata taattggagg aacatattct
      361 attagtacat ctgtcgaaaa ttcattaaaa tctaagaaaa tgaaagttgt aagaattaag
      421 ggaaatgaca gaataaaaac aagttataat gttgcaaaag aaataaattc aataaaaaaa
      481 gtcaatactg ttatgctaac caatgcatat aaaggagaag cagatgctat aagtattgca
      541 tctgtagcag caagagataa agcaccaata attcttacta atggacaaag tatacctttt
      601 tcaactagtg gtttaaagtc ttatgtaata ggaggaacag cttcaatgag taccacttta
      661 gtaaacaata ctaaatctac tagactaggg gggagtactc gttttgaaac taataaagca
      721 ataataaata agttttataa ggatgctaga gagttttata tagctggagc ttatgaatta
      781 acaaatgcac ttgtaggatc ttctttatca aaacatggtc caatggtttt agttaacgat
      841 ggtagcaata aaagcatttt aaaaaatgct aagaagataa cttctatagg atatatagat
      901 tcaaatatag tacaacagtg tttaaatata acaaatggaa ttggagatat taatactggg
      961 gttgtaaaaa atgttaaacc tactacaaag actataaaag atggaatgta taaggttgga
     1021 aaagatatct cagcaggtga atacttaata acttcaaact caggaagtta tgcttcttat
     1081 tatgaagtta caagtgatag tacaggtaat gctgatagta tactttcaaa tgatattttt
     1141 agtgggacta gatatatcac cttaaagaat ggtcaatata ttaaaattga ggattcaaca
     1201 atgacattag caaagtatgc taaagcccag aaggccaaga atggtaagtt tggaaatggt
     1261 atgtataaaa ttggattaga aattcctgct ggagaatata ttataatgtc aaattcttct
     1321 gatgcatatt atgaagttag aaatgatagc ttaggtaatg cagagggaat agttactaat
     1381 gatacttttt caggaagaag atatataacg gttgaggaag atcaatattt aatattaaat
     1441 gattgttatt taattgaaaa tgaatagatg taattgtatt taatagtaaa tcatatattt
     1501 tatttaagga aaggtgtttt aagttttaat ttaaaacatc tttttattat tataataatt
     1561 atatgatttt atattttaga aatatagtat atataaatca ttagatttac tattaaaaca
     1621 caactagaaa aataataata tctttaaaag atttttctta tttgatataa ttaaggttat
     1681 gtgtaatata agaaattaac taggaggaat agacgtgagc aaaagaaaac aagtaacaat
     1741 acctatagag aaaatacaag aacaagataa atatatagaa caaatacaca gacaaaatga
     1801 agaatacttc aacagaactg gaaagagaaa actcgtgttt actcaaacct tcgggtgtca
     1861 aatgaatgaa catgattcag aaaaattatg ttcaatgtta gaagagatgg gataccaaat
     1921 gtcaatgatg gttgaagaaa gtgatttaat aatatataac acctgtgcag ttagagaaaa
     1981 tgcagagcta aaagtgtatg gtaacttagg acaattaaaa catctaaaag gaaaaaatcc
     2041 agatatgaaa atagctgtat gtggatgtat gatgcaacaa ccacatgttg tagaggaatt
     2101 aagaaaaaaa tataaacatg tagaccttat atttggaaca cataatctat ataaatttcc
     2161 gcaattactt acagaatcaa taaattctga caaaatgtta gtggatgtat gggatgtgga
     2221 tggagaagta atagaaggtc ttagaagtaa tagaaaattt gaattaaaag catttgtaaa
     2281 tataatgtat ggatgcaata atttctgcac atattgtata gtcccataca caagaggaag
     2341 agaaagaagt agaactcctg aagatataat taacgagata aaagagcttg ttgcaaatgg
     2401 aacgaaagaa atcactttac ttggacaaaa tgtagattct tatggtaaga cacttgaaaa
     2461 tccagtttca ttctcagaac ttttaagaaa agtgaatgat atagaaggaa ttgaaagggt
     2521 tcgatttatg acatctcatc caaaagatat atctgatgaa gtaatatacg caattagaga
     2581 ttgtgataag gtgtgtgagt ttttacatct accaatacaa tgtggaagca gtagcctttt
     2641 gaaaaaaatg aatagacatt atacaaagga atattattta gaaataatag aaaaagctaa
     2701 aaaggaagtt ccaggtattg ctttttctac agacttaatg attggtttcc caggagaaac
     2761 agaagaagat ttattggaca ctttagatgt ggttgaaaaa gttagatatg attcagcttt
     2821 tacatttatt tattctaaaa gacagggaac tcctgctgct aaaatggaaa atcaaatacc
     2881 agaagatata aagcatgata gatttaatag ggtgttagaa gctgtaaata ggatttctgc
     2941 agaaataaat gatggatata aagatagaat agttgaagtt ctagttgaag gaagaagtaa
     3001 aaataatgaa aataagtttg ctggtagaac tagacaaaat aaactcgtaa atttcgaagg
     3061 tggaaatgat gatttaattg gtaaattagt tatggtaaaa attactgaac ctagaacttt
     3121 ttcattaaat gggatattag taaataatta atattgtaca aaataataaa attatgtaaa
     3181 ctaatcaaaa tagatgtact atagaacaaa ttatattaaa aatttttatt ttaagcgaat
     3241 ttgtcatatg ttatgctaat gtaagttttt gctattaata aaaataaatt tgatataaga
     3301 tataatttca ttgtacatct atttttgttt tattatattt tataaaagct tacctagttg
     3361 tcattagtaa ttccctgtta tcatattaaa ttatatatag tggactgtaa ccacaaaggt
     3421 aaaatcatta ttagaagttt gtactatatt cattattttt acatagattt ttaaatgtta
     3481 aaatatttaa taagagtagt caatatacat gaaaaagcag tcaatctttt ataattaact
     3541 gctcaactta caactattta ttttttacat tttttgttag agtagaggat attaaaaaat
     3601 acactggaaa gtatgactat tataaatatt atagaaaagg gaaccatttt tttttcgtcc
     3661 atttgaccac tctctattct atatcccaaa aacgccataa taaatagcat tatagagaca
     3721 ataaaagtaa caatgctaga acttctacat aatttttttt tatcgtatcg tgctttttct
     3781 ttcttactca tagagttata acctgctagt agcatactgg cttttccatt aaataatagt
     3841 acacttaaaa tgaaaaaggc aagtgcaatt ataacaacaa atcctacagc aaacattata
     3901 agcacctcca tatattttaa ttaattttat tacttattag taattttacc ataaaggcac
     3961 attaaaatca tttacttcat actttattaa atagattatc atactttaat tcatcaacag
     4021 tatacactaa tacatagtat ttatgttgaa attgcattga aaaccatcaa atattcaaac
     4081 aataataaaa taaaaaagtg ctacaaaata tagttattga tattttagaa ttgttaaatt
     4141 tagaaacttt tataaatttc ttatgtaaaa gttttgatga tttattatga gtgttattct
     4201 gataaaaaat ttaaaaagtt ttctagtatt tttgctgtca ttccccaaat tacataatct
     4261 ttatatttat aaaacagaat aggataactt ccactttcaa atttataatc tttttggttt
     4321 ggaattgaac catatggtag attttcgtct ggaaaaactt ttacagtatt actataagta
     4381 tttggaggat tctctaaaag atattttata ggaaccaaaa aagtatggtc aacttcatcc
     4441 ttgtttatat ttaaattatt aatatttttt atatatccta agtatggatg tatgataaac
     4501 tttactggtg ttataaggag gtcaagactt gatattactt ctatattgtc actaaaagtt
     4561 ccaagttcct cacaagtttc tctaataaca gcttcatatg gagtctcacc tgattctatt
     4621 ttaccacctg gaaaagaaat ttcactaggt tgatgcttca aattttttga gcgaacttca
     4681 aataatatat agtgtgtgtt atttatttct actattggaa ttaaaacaga tgctctcttc
     4741 atattcttgt atccatttat atatggtttg taatttttga atttattttt aatatcgttt
     4801 atcattttta cctcctaaaa taatattatt aaaaattata taattgagtt ttaacaataa
     4861 tatataatta tgatttatct aattataaga tatagtaact atctatatta ttatataata
     4921 taatgataaa tatatattaa caaataataa aaaaattaat ttgattgtat ataaattagt
     4981 ttgttattaa attagtatta aaaagttcaa aaatttactt agaaggaatc tataattaga
     5041 tttctatgtt tgttgagaat gcaattacag cttttccaat tatttttact tcaactggtt
     5101 tattatctaa aataattact tttactaaca tcttgccttt gcgttttatt gtttctcctt
     5161 gaactgcttc aaatataaac tctgtgttgg gtgtaggtat tactttataa tgtacaaggt
     5221 acgcacctaa gggtccatta gcatttcctg taacagggtc ttctgaaata ccaattgcag
     5281 gagcaaacat tcttccatga acttgtagag tttctttatt atgtaatgta aaaatataat
     5341 atccattaca ttttatttct tgacttattt tcgataattc atataaattt ggagataaac
     5401 tatttaatgt tgataattcc tttattccaa tcataacttt tgaatgtcct gtagatgata
     5461 tggcaatagg acaatctgta cgaatatctt cctttaaaat accaaatgca ttaagtaatc
     5521 ttttttgata ggtttgttgt aacggtggag atacttcaat ttttccttgt gtcataatta
     5581 tttcataatc attatctttt tttataatat caactggtaa tattccagca ctagtttttt
     5641 gaaatactgt tttgttattg aattggtttt ctactgctct aacataatgg gcagcaatag
     5701 tagcatgacc acatatagga acctcattag ttagtgtaaa aaatctgata tgtacatcat
     5761 aattttcttg atgattttta gccaagttat tgaaaataaa agcagtttct gaattattta
     5821 attctcttgc tattttttgc atttgtgatt cagataagcc atcagcattt gtaattacac
     5881 cagctggatt acctgtaaat ttttcttttg taatgagtca acttgataaa gacgaaattt
     5941 tttattcata ttatataatc ctttctaaac attagattag tttatctaca tagaaaactt
     6001 gagataaatt attatttatt attatctgtt taaataaaat taataaaata acatatgtct
     6061 atattaaagt ttaaacatat acatctaaaa aagtagtatt atgtaaggct ctatactaca
     6121 atcaagatta tattacgaat tagtacgcta aacaagaatg cactttttag ttagatactt
     6181 actattttga aagagaggat tgagatggat atagataata attttgaaga atttccaaat
     6241 attgaagaaa gacaaaaaga ttttaattgt tcaattggtt ttaccatgac tgtgatagga
     6301 agtaaatgga gggcaattat tttatggcat attttaaaac aagagccaat acgttacagt
     6361 caattaaaaa aagaagttgt tcatataagt cataagatac tgtcacaaga attaaagtat
     6421 cttgaaagag atggacttat taagaggatt tcttatccga ctattcctcc aaaagttgaa
     6481 tatttgtcta ctaaacgtgg taaatcatta gaatctatat tattagaatt atgtaattgg
     6541 ggtaaacagt atatgagata aactcaactg tttactttat gaaaaatata taatgaagct
     6601 tattttaatt agttctttaa tgaattataa aacgtagata aaactaaaaa gggttttcta
     6661 gaaaataaaa tttctttaat tatatctgct taccaatacc atcacttgat gcaaacaaga
     6721 ttctaccaca ttctctccat attctagtat aaaatcattt aattgtgtat ctataaaatt
     6781 gcagtaatcg cgtgaactgc atatatatgc agttagtata tgtgtacatc tatccatttt
     6841 atccataaac atatatcata tatcccccta aaaaacattc ataaaaacaa aaagaaaata
     6901 tctctttggc aactggctga cataactcat ttcataagtc ttagtcccta tagctttgcg
     6961 tcactaaatt tctctagttt tgcctattta gttttattct actattacca taatatagga
     7021 aaacgtttct tttttcaagg tgcatattaa aaaatttaaa aattaaaaaa atattttatt
     7081 tctcaaaaat aaattggata aaataattat attatagagt ttgattctta tctaaaatgt
     7141 tggttgctga ggattttgat gataatgatg agtaattttt atattatcac aaaagatact
     7201 tcctaaaagg cttgtatctt aaaaatctag atagaggttt atttttagat tactgaatat
     7261 tattaatgaa tttattgaag gattttttgt agataaagat aagtagagtc taaaagctat
     7321 ttctaatctg taatttgtaa cgattgcaaa ttagatgagt gaaaacagaa atttagcaca
     7381 atatttgtat ggaattgata tacatccaaa tatgcttgaa atagcaaaat aaaaactaga
     7441 aaattcagtt aatctagttt tggaagatgt tgataaatta ccattttaag ataatttatt
     7501 tggtgtaata atgtacaatt tatattagat atttttgaac ggataaaata tctaatataa
     7561 ataaaaaatt tgttaaaata tgataagtgt agtataatca agtagaaaag atataatata
     7621 tttcggggag ctaatgtgct gagaggagat tttatctcga ccctttgaac ctgtttggat
     7681 aatgccagtg tagggagacc agttaagata aaaataagtc ttatcctatt ttggataaga
     7741 cttttctttt atttaaatat aatatctttt tagtaataaa attttttaat aaaaaacaaa
     7801 atatacctag taggtattaa ttatttttaa aggagtgatt taaatggtaa tcgcagacat
     7861 agcagtaatg ccactaagac cttacaaagg tgaagaagag atgtataaag tagtagatgc
     7921 ttgtattgct gaaattgaaa aaagtggtct taaatgtgaa ataggagcaa tgagcactac
     7981 tgttgaaggt gattttgatg aagtttttga attattaaaa aaggtacata aaatcccttt
     8041 taatcttgga tgtgaaagag tcataacagt agctagagta gatgaaaaag ctggaggtct
     8101 gactatagat gaaaaactta gaaatcatag ataaagtcaa agatagtata tatccaatag
     8161 caacattttt gtttatactt attctttggc aatctatggt aggagtttta gaagttcctc
     8221 agtatatact acccacacca gtagatataa ttaatgtgtt ttttaaagat tatcaaaatt
     8281 tatccataca tgctgtggtt actataggag aagcaatact tggttttata gtcgcaatta
     8341 taatatcatt ggtaattgga atattgatgg attttgtatc aataataaaa aaatgtcttt
     8401 atcctattat gttggttaca cagatgatac ctacaataat tatagcacca ctctttatga
     8461 tatggtttgg atttggaaca atgccaaaag tattgatggt aatgcttact tgtttttttc
     8521 ctatattaat aagttttgta gatggcattg agaatattga taaagattat ttaaatttat
     8581 ttaagactat ggactcaaat aaagttaata cattcattca ccttaaattt cctatggcaa
     8641 tggataaatt tttttctgga cttaaaatat ctgcaacgta tgctgtaatg gcagcaactg
     8701 ttgcagaatg gctaggtgga acgaaaggac ttggtgttta tatgcttagg tcaaaaagtg
     8761 catatgcact tgataaagta tttgcatcta ctatattagt agttatattt agtttaatgt
     8821 ttgtaggaat tgtacaggct gtaaaaaaag ttgttataag acatagatta atagattaaa
     8881 ggtatttgag ggaggcaatt atgaatttta aaaagttagg aatattaggt ttagtaagtg
     8941 tttttacatt ggcaacaata actggatgtt cttcaaataa caataaagac aaaaacaatg
     9001 ataaagatac ttcttctaaa aaagtaacta tggtgcttga ttggacacca aatacaaatc
     9061 atactggatt atttgtagca cttgataagg gttactataa agaagaaggt ttggatgttg
     9121 aaatagtaca accacctgaa agtggagcag aaactttagt agcaacagga aaagctgact
     9181 ttggtataag ctatcaagaa caagttactt atgcaaagac tagtgaagat ccacttccaa
     9241 taaaagctgt agcaactgtt atacaacata atacttctgg gtttgcatca ccaaaagaaa
     9301 agaatataac aacagctaaa gattttgaag gaaaaactta tggtggttgg ggctctccat
     9361 cagaagaagc tgtttttaaa gcagttatga agaaaaatgg agctgatttt aataagttaa
     9421 agatagttaa tactggtcaa gatgatttct ttgcagctat gaaaactgta gattttgcat
     9481 ggatatttga aggttgggat gcagttaaag ctgatttaat tggatatgac ttaaacttta
     9541 taccagtaaa agatttagat gagagattgg attattatac accacttata atttctaatg
     9601 aaactgtgct taaaaataat ccagagcttg ctaaaaaatt cttaaaagct acaacaaaag
     9661 gttatgaata tgcaataaaa aatccagaag aatcagcaaa aattttagta aaacatgcac
     9721 ctgaagtaga tgaaaaacta gctttaaaga gtcaagaata tttagcaagc aagtacaaag
     9781 atgatgcacc acgatgggga gaaatgaaag atagtgtatg gaacaactat acatcattct
     9841 taaaagaata taaattaata gataaggata tgaaggcaag tgatgcttac acaaatgaat
     9901 ttttaccaca ataaaaaagt aaaagtatct atagaaaatg tatcaaaaac ttataaagat
     9961 attgaagttt taaaagatat aagtattgat gtttatgaag gtgagtttgt ttctatatta
    10021 gggccaagtg gatgtggaaa aagtactatt tttaatatta taactaaact tacagattat
    10081 gatagtggca aagtgaatat aaatggtaag tatagttata tgtatcaaaa agatttactc
    10141 ttgccatata aaactataat agataatgtg tcactaccac taattttaaa aaaagaaaaa
    10201 aagagtaaag ctaggtcaat ggtaaaacct tattttgagg tgtttggatt aagcggatat
    10261 gaagataaat atccttcaga actttctgga ggaatgagac agagagctaa cttcttgaga
    10321 acttttataa attctaatga cataatgctt ttagatgagc cttttggggc acttgattca
    10381 ttaactaaaa catccatgca aaaatggctt ttagatgtta agaaaaaagt aaattcaaca
    10441 atacttctta taactcatga tattgacgaa gctataatgc tatcaaacag aatttatgtt
    10501 atttcaaaaa aaccatcaat agtaaagaaa gaatttgtta tagatagtag aaagttaaat
    10561 gaagataacc ttgaaaatat cattaaactt aaaaaagaaa taatatcttt attgtaaaaa
    10621 aaaatatttt tatttaataa gcattaaact tagcaatcca aaatgtgagt atacattttg
    10681 gattgttttt agttatatgt caaaatttta tatattgtgt taataataat catctactat
    10741 gatataatat ttaattagaa aagaaggagg tttgataatg caaatagata tgaataaatt
    10801 aactcctatg atgaaacagt atcttgaagt aaaaaataga tataaagact gtatactgtt
    10861 ttttagactt ggagattttt atgaaatgtt ttttgaagat gcattagtag cttcaaaagc
    10921 tcttgaaata gcattgacag gaaaagcttg tggtttagaa gaacgtgctc ctatgtgtgg
    10981 tgttcctttc cactcagcca attcatatat atcaaaatta gttgaaaatg gatataaagt
    11041 agcaataggt gaacaaatgg aagacccatc tacagcaaaa ggaattgtta gaagagaggt
    11101 tataagggtc atcactcctg gtacagtttt agatggaaat ttacttgaaa ataaaaagaa
    11161 taattattta ttatctttgt ataaggatgg tactaatata ggtctaactt atgttgatat
    11221 aagtacagga gaaactaatg caacatgttt aaatgaagat aaggtaattg aagaaattgc
    11281 aaaaatccat ccaactgaaa taattataaa tgatttagac tttatagaaa agcttagaga
    11341 tatagctact gtaagtaata tatacataaa tgaaagtttt agtgataact atttggatat
    11401 aaatatatta aaagaatatt ttccagatgt ttatctacaa aaactaaagt ttgatgataa
    11461 aggtctaata aagtcaagct tatcaattct tctgaattat atata
//
LOCUS       DACULQ010000124        11386 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.125, whole genome shotgun sequence.
ACCESSION   DACULQ010000124 DACULQ010000000
VERSION     DACULQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.125"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            64..255
                     /locus_tag="JA154_13410"
     CDS             64..255
                     /locus_tag="JA154_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996843.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(344..1156)
                     /locus_tag="JA154_13415"
     CDS             complement(344..1156)
                     /locus_tag="JA154_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAT7996844.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            1578..2729
                     /locus_tag="JA154_13420"
     CDS             1578..2729
                     /locus_tag="JA154_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7996845.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            2847..3485
                     /locus_tag="JA154_13425"
     CDS             2847..3485
                     /locus_tag="JA154_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HAT7996846.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            3486..4508
                     /locus_tag="JA154_13430"
     CDS             3486..4508
                     /locus_tag="JA154_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HAT7996847.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            4498..5118
                     /locus_tag="JA154_13435"
     CDS             4498..5118
                     /locus_tag="JA154_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996848.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKVGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(5242..5928)
                     /locus_tag="JA154_13440"
     CDS             complement(5242..5928)
                     /locus_tag="JA154_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAT7996849.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(5942..6487)
                     /locus_tag="JA154_13445"
     CDS             complement(5942..6487)
                     /locus_tag="JA154_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT7996850.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            6715..7962
                     /locus_tag="JA154_13450"
     CDS             6715..7962
                     /locus_tag="JA154_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HAT7996851.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            8049..10043
                     /locus_tag="JA154_13455"
     CDS             8049..10043
                     /locus_tag="JA154_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT7996852.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNYCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            10043..11365
                     /locus_tag="JA154_13460"
     CDS             10043..11365
                     /locus_tag="JA154_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAT7996853.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
BASE COUNT         4569 a         1190 c         1835 g         3792 t
ORIGIN      
        1 gataaactct gcattaagag gatatatagt ttatcttaat tataggggaa gtgaggatat
       61 attatggaaa gattaatgga actcatagaa agaaaatttt taaaggatgt agaactttta
      121 gaattagaag acatagaaaa tgtaatagaa tgcaaaatga cacagccata taatgctcat
      181 tataatgaat atttagtaaa agtaaaaaat ggagatgaag tagaagaata ttttgtgtat
      241 ttaaaacata gataaacata aaagaggttt atatttattt gtatgattat aaaataaata
      301 caagcctctt tttttgataa atataaaatt ataaaatttt tatttattta attttttcaa
      361 caacattagt caatattgaa tctaaatcat cactaatttc tgtaactcca ttatgagtaa
      421 tatctataat ttctttagtc ataggaagtt ctcctaaaag ctctaagtct aaatcatcta
      481 aaaacttttc agtagattct ccttcaaata acttgatttt tttagaacaa tcaggacatt
      541 ggatataact catattttca ataactccaa ggacatttat gttcattttt ttagccatat
      601 taacagcttt tgatacaatc atagatacca aatcttgagg tactgaaacc ataactattc
      661 cacttatagg aatagattgc ataactgtta aggcaacatc tccagtacca ggaggcatat
      721 ctattaataa atagtctaat tcttcccaaa gtacatcagt ataaaattgc tttacaaccc
      781 ctccaagtaa aggtcctctc caaacaacag gttcattttc atcatctatc ataaggttta
      841 tagacattac ttttatgtta tttgagtttt ctacagggta aatataagag ccatcagaat
      901 aagcttttac attttttaca cccataagtc taggaatact tggtccagtt atatctgagt
      961 ctaatatacc aaccttatat cccattttgt ttagcttatt agcaagtaaa gcagttacag
     1021 ttgattttcc aactccaccc ttcccactca taacaccaat tatttttcca aatttattgt
     1081 tagggttatt ttcaatagaa caatttgatt gactattaca atttcccttt gatggacatg
     1141 aattacaatc tgccatatct attacctcca aactttaata tttattagat tatttgtaac
     1201 ctacatatac tattttagta caattgaacc ataaagtcat tatttaaaag caacttagaa
     1261 gattttctat tgatttattc atagactata gcaaaaagaa tacaataaaa aaattataac
     1321 attcttgaaa aagtgattat ttcaatttag ttatagaact tagtcatata aatatataga
     1381 aaatatttta atatattata cataacgggt tattttataa gtttaactat aaattaggtt
     1441 tatattttat aaaaatttgc taaaaaaaac aattaaaaat tgtaaagaaa aaagatataa
     1501 ttagaactgt agtaataaat gttttattat agataataga gttttaagta tcttaaataa
     1561 tatttggagg aatatgaatg gaattaagac caagaagtgc aaaattagaa gaatttgata
     1621 aagtaataga attaataaac tatgtattta gaatatcaag aaatcataaa ccaactatga
     1681 tggaagaatt tcctctttta ctaagtaaaa ataatataga aaatatgatt ataatatcag
     1741 aagatgataa agtggtttct gatgtaaact atctaattca ggatgtatct attcaaggaa
     1801 acagattgaa ggttgcagct ataggtggag tatgtactca tccagattat gaaaagagag
     1861 gctattcatc taagatattg gataaagtag aagaaaaaat gttttatgat ggtgtagata
     1921 ttgtaattat atcaggaaca agaagtctat acagtagaag aaattgtagt ttggtaaaga
     1981 gtttttataa atatacaata aaacctgaag atgtaaaaat agcatatgaa atagttgaat
     2041 ttgatgaaac taattttgaa aaagataatg atttagataa aatgattgaa ctatataatc
     2101 aaaatagtac tagatttata agaacgagag atgagtttca aaaactattg catgcagcaa
     2161 ctattgcctg gggaccaatt ggttacaaga aggtatttat aaaggaaaat aataatataa
     2221 taggatattt gataataaga acaataaaaa aagaagattc tactgtagga gaagttgctg
     2281 aaatagggtt aaactctgta aatgttgaaa atatattgaa atatgttgca aataaatttg
     2341 gattagaatc tttaaattat aaagtacatg tgaaaaatct taaagaccag ttgaaatgta
     2401 atgggacaaa atctttggat tatcaacaag gaactatgaa aataataaat tttactaagt
     2461 tgtgtgatag tttaagaagt tattttagtc aatatgtaga ttttgaatta ctaaaataca
     2521 tggaatttaa acaagttgaa aataaatata ttattaaata caaagaagaa gaacttgtaa
     2581 tagaaaattt ggataagctt aataaactgt tttttgaaaa aaatgaggaa cagtacaatg
     2641 aatttaaaca tctaaaaaat atatatgaat ttgctacaaa ggcatttcct gtagattttc
     2701 catggactgc aaatttaaat tatcaatagt tttagatgat attaaataaa attgattgat
     2761 acctagagtt agtccttgtt aaataagaga aattattgga tattgaaaaa gcaattttgt
     2821 aagtaattag aaaggatgag tattatatgg acatacttga tgatatagca gaatgtatat
     2881 taaatatggg tgtagataat atagagaatt tagtaaaaat tgctatagat aaaaatattg
     2941 atgtggaaga tatttatgaa tatggtttaa acaaaggtat gataggagct ttagataagt
     3001 ttgagaataa ggaatactat ctttcagaag ttatagtatg cacagatgca ttaaataaag
     3061 gtattaatct tttgaaggga actggaaaag taaagaaaaa atcaaaggga gtaattctga
     3121 tgtctgtagt agaaggagat actcatgaga taggaaaaaa tattgtaaaa gtaatggtag
     3181 aagccactgg atataaggtt attgacttag gtgtaaatag aaaaagtgaa gatataatag
     3241 aagaagctat aaaaaacaat gtagatataa taggcttatc ttcaatgatg acaactacaa
     3301 tggaaaatat gaaatcggtt atagacgaac ttaatatgat tgagatagat aaaagaccaa
     3361 aagtaataat tggtggagga cctgtaagta tggagtttgc agaagaaata ggagcagatg
     3421 gatacagttc aaatgctcca aaagcagtta aattaataaa taaacttata ggaggtgaag
     3481 tttaaatgtt ttcagatatg actttacttc agtatataaa tagtgaagat agaggttttt
     3541 ttctgcctga tatgggtaca aatggattgt tcttaataaa taaaaaggca tatgaagtat
     3601 atgatagtgt agatttgcaa ttagaattag caaagactat ggacaagtat tttgaatcag
     3661 attttatata ctcattctgt gatgggatta cattttgtga aactctgggt ttagatacat
     3721 taagaccaga ttatgatttt ccaagtgtat tgacacatac tataactgac agagataaat
     3781 taagaaaatt agatgttcca gacccttata cgagtggacg tatgccactc aatatagaga
     3841 gcttatcagt tatatcaaaa agtattaaaa aaccactata tgaatcgata caaggtccat
     3901 ttacacttgc aggtcaatta gctggagcta ctcaactttt aagatgtata ataactgata
     3961 aaaaatttgt agaagaatta ttggaattta caactgaatt ggtaagaaga tatgctgtag
     4021 ctgcaaataa agcaggagca aaatatattt caatagcaga gccaacatca gtaaccctaa
     4081 gtaaagatag atttgatgaa tttatagtaa aaaatttaaa taaaatctac aatgaacttg
     4141 attgttggaa aggtatgcat atatgtggag acaccagaga attattagat aatatgctaa
     4201 gttgtaatat agatgcagta agtttagacc aaattttaga ctatgaagaa attgctccaa
     4261 tgattcctag agatatagtt ttgattggta atttagaccc aataaaactt cttggacgtt
     4321 ctaagccaga taagattaga agagagacac taaaattgtt aaagaagatg agaagatatg
     4381 ataacttttt atgtgatttt ggctgtaact gcttaaatac cactccagta gaaaatttac
     4441 aagcagctat aaaggcagga agaattagtt ataaagaatt agataggatt gatattgatg
     4501 aattataata gtaataaaaa taaatatctt ttaaaggata taaatttaga tgatattttt
     4561 atagataaga atattgtgct aaagtttttg gggtatggaa atagaaaagc tccagataca
     4621 attagtaaaa tcgttgaaag agaaataaga aatataaatg atattttaga tatcaagata
     4681 tatataagtg aagttgatat taataaaaca cctaaagaat gtaaaaaagc atttgctgta
     4741 ctatatacaa taggggataa aattgatttt aagatgaatg actatatgga aaattgcaat
     4801 atgatggcag ggctggcatt ggataaggta ggtatagtat gtttagattg tattaatgag
     4861 aaaataaaaa tgtatttaaa aggaaagtat gatagtctca aaatatcgca tgaaatatat
     4921 ccaggagata aagattttga agtagagaga caaaaggata tttataacta tgtaaaaaat
     4981 aaatataata gtatagaaat agaaattaat gattatcatc aactatcccc cataaaaagt
     5041 gttgcaatgc ttattttaat gggggatgaa gaaaatttag aatcaagatg tagtaagtgt
     5101 cctagaaaat gcttttagtt aattttcaag actaagattt gttttgtgta taaattgcaa
     5161 taattttttt ggtcatttta tgtataaact aaaaaaatta ttgcaaagta atattctatt
     5221 taatttgaac agacttacaa actatagtag tttctttaat tcaataagtg tatttatttc
     5281 ataagttggt atatgattag tataatttat actgttattg gaatttaacc aacaagtatc
     5341 tattcctgaa tttattccac ctaagatatc agaggttaag ctatccccaa ttattaaggc
     5401 agaagattta tcatgacaat taaacttttt gagagcgtat ttaaatattt caggatttgg
     5461 ttttgatatt ttcaactctt cagatacaac cattccatca atatattctt ttatttttga
     5521 attttcaagt cttttctttt gaactgcaga aattccattt gttattataa caatagtata
     5581 ctttttttta agatattgta atatatcgat tgcacctgga attaaaaatg tacattcacc
     5641 taaaaaattt aagtacattt tgctaagagt ttcagaatca acatccaaag atattttatt
     5701 tgcaaaaagt tcaaatctaa gtaatttaag ttcattcaaa gttataatat ttttttcaag
     5761 gtcgagccaa agcttatcac ttatatttct atatgactct atataattct caaaattaaa
     5821 ttctaaatca aactcagata gaagtttttt aaaagcatgt gattcacttt tcttaaaatc
     5881 aaataaagta tcatctgcat caaaaaatat aaatttatat gtcttcatgg tttttactcc
     5941 tttacaagat gactgcaaaa attactttac tttcactatt tgagtcattt tgccatttat
     6001 gttttgttcc taaaggaact tttacactat ctcctttgga caaagtaaat tcatcatcat
     6061 ccataaaaag tttaacttct ccctccaaaa catatgcaat ttcatctcca ttatgagtaa
     6121 tcctatcaac acaagaagaa gtgtttggag ctaaatccat aattgcaaat tctaaattac
     6181 cactagagtc tggaattaaa atctcatata taacttcttt gcttccaggt aaaataactt
     6241 tttttctatt atcatgtctt actatcaagt tttttttatc tacctcagat acaaaaaatg
     6301 taaataaagg aacgtttaga gaagaggcta tagattttaa tgaatttaca gatggattag
     6361 caagacctcg ttctatttgg ctcaataatg atggtgtaac atcagctagt tttgctaaat
     6421 ctcgaatact taagttttgt ttttttcgaa cttcagctat tttttcccct aaatttaaat
     6481 cattcatata aatatcctct ctttttaatt taaaaaatat taacttaaaa taatcaagta
     6541 aaatgattat gtagtatgaa attattataa catatagaat taggttttga aaaaactatg
     6601 ttcaatataa ttaaataaat taaaatataa ttgacaaagt ggatacagaa tacttaaaat
     6661 atatttaaat aaaattaagc aaaagatagt taatttatga taggagggga tattatgcaa
     6721 ataacgactt ttggagctat atttggttta ttaatagcaa ttattttaat tataaaaaaa
     6781 tttcaagctg tatatagttt aatgctagga gcatttattg gaggattagt tggaggagca
     6841 aatataacac aaactgtaga ttttatggca aatggagcta tgaatatatc accatcaatt
     6901 cttagagctt tggcatcagg tgtattagct ggaagtttaa taaaaacagg ggcagtagat
     6961 aaaatatcag aacagattgt aaaaatattt ggtgaaaaga gggctttatt ttcgattgcc
     7021 atttcaacta tggtacttgc tggagtaggc gtaaatttgg atgtatctat aataactgta
     7081 gcacctatag gattatatat aggtagaaaa ttaaattatt ctaaattgtc aatactatta
     7141 gctatgttgg gagggggtaa agcaggtaat ataatatcac caaatccaaa tactatagca
     7201 gtagcagata atttttctgt caatctctct agtgttatga tggcaaatat aattccagca
     7261 attattggag ttgttattac agttatttta gcaagtatat taataaataa aggaaataaa
     7321 gtacaatcat atgaaatttt agagcaaaga gaagatttac caagtttatt taaatcatta
     7381 tgtggtccaa ttatagctat atttctattg tttttaggaa atgtatctcc tatagttata
     7441 gacccaatga tagcccttcc aataggagga atcgtaactc taattgtcac aggaaacttg
     7501 aacaattcaa gagaatattt agcttttgga ttatctaaga tgcaaggggt atgcatatta
     7561 ttacttggaa cagggactat cgcaggaatt atacagatgt ctgaattaca acagagtaca
     7621 ataggtgctt tacaattttt aaatatgcca caatttttat tggcaccagt ttcgggtata
     7681 cttatgtcat tagcaacagc atcatcaaca gctggtgcta ctatagcatc atctactttt
     7741 catgatgcta taataaatgg tgggctatca ccgatttcag gtgcctcaat agtaaatgct
     7801 gggtctagtg tattcgagca attaccacat ggctcactat ttcatactag tgcaggtagt
     7861 ataaacatgg atataggtga aagatttaag ttaattccat atgaagcatt gataggaata
     7921 gtaatgacta taatttcaac atctattcaa ttagttttat aataaatata aatatttaat
     7981 gaaaaaagta ggattttgtt attaaatatt gaatttatta tagtaaaaaa gaagattgga
     8041 gttttaaagt gaataaaaaa aagattgtta ttatagggat tatttattca tttttagtag
     8101 tattttcact tacaaatatg tatgtaaata tggagtataa tctaaatgta tttgaatata
     8161 ttaagaagtc acttccattt acagaagaag aaaaaaagtg gttagaaaaa cacaagaatt
     8221 tgatttacag ctctgaccaa agttcacctc ctttgagata taaaggtaag gaagatggac
     8281 agtataaggg aattattgtt gatttaataa attctctgtc aattcaaatt ggtagagatt
     8341 tttattttaa accaaataat tggtggaaag aatctttcgt aaattcaata gatgatagta
     8401 taaagttttt tgacttgatt ccatcaaaag aaagggcaaa taaatttata tttactgacc
     8461 caatatacac tttgagtgca aatatattga aagataaaaa gtcccaagat ataaatagtt
     8521 acatggattt aaaggggaaa acagtagcaa ttccagaagg tgattattct ataaattttt
     8581 taaagcaaaa aattcaagat ataaacattt tgttgactcc agatataaag actggagtca
     8641 accatttaat gtctggaaaa gtagacgcag ttgttggtga tgaaccagta ttgcgatact
     8701 atataaataa ttatggactt tcaaataaat acagtgtttt aagtaatcct atatatacta
     8761 aaaaagcagt tcttgctgta cctaaacaat atgaagagct ggttagtatt ttaaataaag
     8821 gcatttttaa acttcagaaa aatggagtgt ataaagattt aaagaaaaaa tggtattcta
     8881 cgtataatga agttgatgat atattgtatg agcgaggaat tgtgcctagt atttatcttt
     8941 ttataggtat aattttaata tcaatttatg ttttttatag ctatacatat ttgttaaaga
     9001 tagaaatcaa aaggaaaaca gaacaggtaa tagaaaataa aaaaacttta gaggcaactt
     9061 ttaatagtat tacagatata ataatgcttg tagatgaaaa taacaatata gtagaatcga
     9121 acaaagtact atatgacttt atgggtgaaa tgagttataa gatagcagat ttgattagca
     9181 tgataaaagg tgttattgaa aataccttct ctgaaaatac taataaaact agtgaaatag
     9241 aaatacacaa taaaatttta aagataaaca cttttcctgt agagtataaa aaaaataata
     9301 ctgagtatat agtagttctt attaaagata taactaatga taaaatagta gaggcgaaat
     9361 tacttagaga aaacaaaatg atatctatag gacaattagc ttctggggta gcacatgaga
     9421 taagaaatcc tttgggtata ataagaaata attgctatct cttaaaagat aacgttacaa
     9481 tggaagaagt aaatgattgt gttaaatcaa tagaaagtaa tgtagatagg gcaagtaata
     9541 taataaccaa cttgttaaat ttcgcaagaa tatcagatga taatttagaa catataaata
     9601 taagaaattt tattgaaaat atagtgaaac ttcaatataa aatgttacag ttaaagaatg
     9661 ttgaaataaa aattgactgt gaacataatt tagtatgcta tataaatgga gaatcgttaa
     9721 aacatgtatt tataaattta atttctaatt caatagatgc aatacatcaa gatggaaaaa
     9781 ttattatata ctgttatgaa aaaaattatt gtttatttat agattttaaa gataatggtg
     9841 aaggaataaa agaagatgcc ctaaaggaca tatttaatcc cttctataca acaaaaccaa
     9901 ttggtgaggg aactggttta ggtttataca ttacttataa tgagattaaa aagaataatg
     9961 gagatataag tgttgaaagt aaattggggg ttggaacttg ttttcatata aaaattccat
    10021 taaataaaga ggtgactata taatgaaaat actattagtt gatgatgagc tagaatatgg
    10081 agtggtaatg aaaaaaattc tacaaaagaa gggctatctt gtagatatag cactaagtgg
    10141 tgaagaagct ataaatataa ttaaaaaaga taaaaattat gatttggttt tgtctgatgt
    10201 tatgatgaaa aatatggatg gagtacagct attagataga attaaatcta ttaatagaga
    10261 tatagaggtt atattagtta caggatatgg aagtattgaa aatgcagttg atgctatgaa
    10321 aagaggagct ttatcttatt ttataaaaag taatcccatt gaaaatttat tagaagaagt
    10381 tgaaaaggta aaggcaagta aaatatctgt ttctgtacaa aaaaataatc tggaatttac
    10441 tttggaatct aaaaatagag attttaataa tatcataaaa atagcaaaaa aagctgcttg
    10501 taaagatgtt aatatattaa tcttggggga atctggtgta ggaaaagata tattagctag
    10561 atatatacat agcataagtc caagaaagaa tgagatattt gtacctgtaa attgttgttc
    10621 tttttcagaa aatctattgg agtctgagtt gtttggtcat gaaaaaggtt cttttacagg
    10681 agcagtagat agtagaaaag gtagatttga actttcgaat aaaggaactc tatttttgga
    10741 tgaaatagga gatatacctc taaatgttca ggtaaaactt ttgaggacac ttgaggacaa
    10801 atctatagag aggataggaa gtaataaatc tattaaagtt gactttaggt taatttgtgc
    10861 tatgaataaa gaaccaaaag tggaaatatc aaatggaaat ataagagaag atttttttta
    10921 tagaataagt acaataacaa taactatacc accgctaaga aagagaagag aagacttaac
    10981 tactcttata gaatttttct taaataagta ccaaattgaa catgataaaa agatacactc
    11041 gattgataag gaagtaaagg attttttatt aaattacaat tatccaggaa atataagaga
    11101 attaaaaaat ataataaata gacttgtagt tttaagtgaa gaaggcaatt tatcaaaaga
    11161 caatttaaat ttaatatcaa ataatgtata catagatgat aaaataagta ttagaccttt
    11221 aagagaaata agaaaagagt ttgaatgtga gtatattgaa aaagttttat ctttgtgtgg
    11281 aaataatatt tcaaatacag ctaaaaaatt ggagattagt agaagacagt taaccaataa
    11341 aatatcagag tataatatta agtgagaaaa aaacttctca ttaagt
//
LOCUS       DACULQ010000125        11312 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.126, whole genome shotgun sequence.
ACCESSION   DACULQ010000125 DACULQ010000000
VERSION     DACULQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.126"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(97..456)
                     /locus_tag="JA154_13465"
                     /pseudo
     CDS             complement(97..456)
                     /locus_tag="JA154_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(783..1313)
                     /locus_tag="JA154_13470"
     CDS             complement(783..1313)
                     /locus_tag="JA154_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAT7996854.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(1401..2495)
                     /locus_tag="JA154_13475"
     CDS             complement(1401..2495)
                     /locus_tag="JA154_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HAT7996855.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADRNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            complement(2497..2943)
                     /locus_tag="JA154_13480"
     CDS             complement(2497..2943)
                     /locus_tag="JA154_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7996856.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            3240..4439
                     /locus_tag="JA154_13485"
     CDS             3240..4439
                     /locus_tag="JA154_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAT7996857.1"
                     /translation="MLHMISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIG
                     NPSVEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTREN
                     LIMTCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEP
                     DLDALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDE
                     PYREIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSL
                     NVANRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTF
                     YLFPKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKL
                     AKLYNLK"
     gene            4609..5571
                     /locus_tag="JA154_13490"
     CDS             4609..5571
                     /locus_tag="JA154_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAT7996858.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(5693..6355)
                     /locus_tag="JA154_13495"
     CDS             complement(5693..6355)
                     /locus_tag="JA154_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996859.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYAEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(6455..6540)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(6726..9644)
                     /locus_tag="JA154_13500"
     CDS             complement(6726..9644)
                     /locus_tag="JA154_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HAT7996860.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(9762..9847)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(9974..11152)
                     /locus_tag="JA154_13505"
     CDS             complement(9974..11152)
                     /locus_tag="JA154_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAT7996861.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
BASE COUNT         3610 a         1816 c         1299 g         4587 t
ORIGIN      
        1 aaataatttc atatacaaat aataaaatag ccactacaat ccataatttg cagtggctac
       61 tttttacact atcacttatt acttaatttt taaaaattaa ttgtattttt taaataattt
      121 atacatacct attatcagta aagcaataaa tacaagtata tctataaaaa taattattgt
      181 aacatgtctg gcaatattgt aaataaaaag tatagagaat atttctatag acaacgctac
      241 aaaacacata aaattcgtta aataatctgc aatagtgtca ctataaaaag catgtatttg
      301 taaccatctc catataaatg gacgtaaaag gattctgaat acaacacaaa tgacagggta
      361 ggcataaata aattttttat ctacataatt agctacacca ttattccatt ggataggaat
      421 ttgaggtggc aactttggaa atactgttat tgcaatttaa acactacctc tctattctat
      481 taatttataa ctataatata gtcttaaata ttttcactct ataaaattgt atatttagta
      541 tatcttaaaa tcaaactaat tatcttttat aagttttcat acaataatat ctcctttcaa
      601 taatatatct ttttttatat ttaaaactat cttattttct ataaaataat gtataaacaa
      661 ggtttacagt caagtatttt tattattttt ttgatttcat aaaaaaagca tagcaaatta
      721 tttgctatac ttttttacta aattatttat tctatatctt atatgaactt attgtttaac
      781 tactacacaa tactagcttt aatcatcata ttttttgcaa acatactggc ttcatatatt
      841 tcttgttcat ctggatgact ttgagattcc tcatggttat ttaattgttg gcgaatatgt
      901 tcgttgtcag gagaaagttt taacatctct ttatattttg ctttcaatcc ttctgctatc
      961 ttaccttgac atatgaatga acctaatatt tcattatctt ttgggataat tgattctatc
     1021 ttttctatta tactagctcc ataacttcct tttttacttg ctcccattgt tccaaatagt
     1081 gctatctttt tattgtgaat attttcaagc acttgttttg aatttttatc acaaattccc
     1141 ttgtccaccc aatagcctaa gaatactata tcataatttt catagtcaat actataatca
     1201 actaattcat ttacatcttt aatgtctttt tcttcttgaa tttcatcata tatagtataa
     1261 gcaatctttt tagtatttcc agttaaactt gaatataaaa ctaaactttt catgatgtct
     1321 cctcctcttt atctaaatac ttatctaaat caccaaaaat atattaacct taacaaatta
     1381 tttattttta taaggatatt ctaatctagt atacatccaa actgagagcg tgtttgtaaa
     1441 gcatctgcac acttcttatt acaataacag cactttttaa tctcatctac tcttccatct
     1501 tttactttct gtaaccaatt cctatctgct agtaattgtc ttgacataga aaccatatct
     1561 actctattat ttgaaataat actttctata aaatctgggc tagacaattt tccaacccca
     1621 catactggaa gatttgtgtg tttctttact tcatcagcta aatataaaaa acagccttct
     1681 tctttaaagt aaggatgatt attggttggt atagtatctt ctaattttga atgatttgca
     1741 agtgttacat gaaaactgtt tacacctagg gattcaaatt ttttaacaaa atactcaact
     1801 tccgaaagta aaactcctgc atttccataa tgaggatttt cttgtcttat tgcaaattta
     1861 tagtctattg gaatgtcctt aacttcatct cggatactac taattatttc gcttgcaaat
     1921 ctacttctat tatcacacgt tctaccatat tcatcatttc tattattaaa tatacttgat
     1981 gaaaaacttc ctacaagcct atcaccatga atttgaatca tgtcaaaacc tgcttttttt
     2041 gcattcaatg ctgttacttt aaataaattt ataatatttt taatttcttc tttgggcata
     2101 tttgttatgt aatcttttac tccgtcattc attatttttt ttatctcatc atgtgaagta
     2161 attcccatat tcatagtatc tttaattaaa ttaacatcat aatctgaaca aaatagttgt
     2221 gctgatacct ttgcaccaga attgtgtatt tcatctacaa ttttcttgta tttcatgact
     2281 ccatcttcat ccgataggct tatagctacc ttatgaaaac taggtctaat agatacatct
     2341 cctagagtaa tcagagctac tcctgactta gcaatatcag aaaacttttt aattttttct
     2401 tccaatttaa gacccatgct tgtaggtgca aaagatactc tattttttaa ttccaaattt
     2461 ccaattttaa ttggttttaa taaattacaa tacatcctat tcttcctcca cagctgtttt
     2521 tatatccttt accacatgta aattctttac cagttcctta tattctgtat ccgagaactc
     2581 tttttttatg tgattatccc actttgaaaa tatgtttttt atatccccta atacttcctc
     2641 acccttatct gttactctca actgaaaagc tctactatca ttagggtttt ggattttttc
     2701 tatgtatcca tcttgttcta acctagatat agttcttgtt gtatgagctt tatcaacttc
     2761 aaattctgta gaaacatcat tcaaagaaca attcctattt ttattaatat aaattaaata
     2821 gaaatataga ccattcgtaa gtttatatgg ttttaaacat ttttcacaat aaagtttaaa
     2881 gtttttttgc aagttacaaa taatatatga caaactttca tactgtaaat ttttaaaatc
     2941 catcttaaat ttcccttcta taattataat atgtttgact atatcaacta tattatatta
     3001 aacaattaat atagtcaaga atattttatt ctctaaatct atgttttatt aattctttaa
     3061 aaaactatta tataaaataa tcatgataaa attagaaatt ggaattttta atatgtactg
     3121 tacataaata tattttatat aaaagtttat attttcaaac aagtaatttt tcgaaaattg
     3181 tctaaattca gtaaaaatat ggtaatatat acataagcaa tataaatcta taaaaaggag
     3241 tgttacatat gatatctaat aaaatgcaaa ctttagttgc aaatagttct gttataagag
     3301 ctatgtttga agaaggaaag aaactttctg atatttatgg tgaggaaaat gtttttgatt
     3361 ttagtatagg aaatccaagt gttgaacctc cagagactat taaagcagtt ataaatgata
     3421 ttttaaatga agaatctcca aatttagttc atggatatat gaacaactca ggatatgagg
     3481 atgtaagaga tgctattgct gaacacataa ataaaaaaga tggattaaat cttacaagag
     3541 aaaatttaat tatgacttgt ggtgctgctg gtggtctaaa tattatttta aaaactcttt
     3601 taaatcctgg tgatgaagtt attgcttttg caccgtattt tggtgagtat aaaaattata
     3661 cagaaaacta tgatggaaaa ttaatagaag ttcctacaaa tatagagact tttgagccag
     3721 atttagatgc tctaaaaaat gctattactc caaaaacaag agctttaata ataaacactc
     3781 caaataatcc tactggtgtc atatattctg aagaattatt aaaaaatctt ggagaattat
     3841 tggattcaaa acaaaaagaa tttaatacaa gcatatacct aatttctgat gagccatata
     3901 gagaaataat ttatgatggt gccaaagttc cttgtgtact aaaatactat agaaattcat
     3961 ttataggata ctcttatagt aaatcattat ctctacctgg tgaacgtata ggatatattg
     4021 ttgctaatgg gcaaatggat gattttgatg atgttatgtc ttcattaaat gttgcaaata
     4081 gaatacttgg atttgtaaat gctccttctc tttttcaaag ggttattgca agaagtttag
     4141 atgcagaagt tgatgtaaat atttataaaa agaattttga ccttctatat aatagtttaa
     4201 tcgatatggg atattcttgt gttaaaccta atggtacatt ctatttgttc ccaaaagctc
     4261 caattgaaga tgataaaaaa ttctgcaatg atgcaaaaca atttaattta ttattagtac
     4321 caggttcttc ttttggttgt ccaggtcatt ttagagtatc atattgtgta tcttatgata
     4381 aagttaaaag ttctttacca gcatttgaaa aattagccaa attatataat ttaaaataac
     4441 taaatcaaag taattcaaat aattaactac cttagtatag aatacttaca ttctatatta
     4501 aggtagttta taattttatg tactatattt tatacatacc atattattcc aatatgcttg
     4561 aatattctta tctatagtac taatataaat tacaaggagg tgtttgactt gaaaaaaggt
     4621 tatgtttata taatactgac taccttattt tttagttcaa tggaaattag tctaaaaact
     4681 gtcactaatg actttaatcc cttacaaata actttatcaa gattttttgt tggcggttta
     4741 gtcttactac cttttgctgt aaaaagatta agagctctta gtctttctat tacaaaatca
     4801 gacttaaaat actttgcatt tttaggtttt atgtgtgtag tagtaagtat gtcattgtat
     4861 caacttgctg ttctaaacac aaaggcatct gtagttgctg ttttgttcag ttgtaatcca
     4921 gtctttgtaa tgcttttagc ttacttaatt ttgaaggaaa aaatatatag gcacaatgta
     4981 atttcactta tattagaagt tcttggaatt atagttataa taaatccatt gcatacaaaa
     5041 ctaactctta gtggaatatt cttaacatta tcttctgcta ttattttttc aatgtatact
     5101 gtatttggta aaagaaaaac cttaaaattt ggtggaatag ttgtaacctg ctttagcttt
     5161 atatttggta gtttggaaat gttgatattg gttttactca ctaaaattaa ttttatcgca
     5221 gatatattaa ataaaaataa gcttcatgta tttgcaaata tccctatttt tagtggatat
     5281 accttacaca atatgcccat tatgatttat gtattcgtat ttgttacagg tgttggttat
     5341 gcgctttact ttatggcaat ggaagctaca tctactagtc ttacatcttt agtattcttc
     5401 tttaaaccag taatatctcc aatactagca ttattaatac ttaaagaaat tatccctata
     5461 aacatggtta taggaatttt attgatagta attggttcaa taatttctat tatacctacg
     5521 ataattactc aaatgcataa taaacacgtt gaagccttgt ataaggagta gtaattgata
     5581 atctatcata gttatacata agacaaaatt tattagattt aaacgcaata taaaagagat
     5641 gctattataa tagcatctct ttatttatat ttattttaaa tttattaaaa ctctatttag
     5701 ctaaattttg caaaaagcta taagtttgtg gacaagctga ttttaactta ctatttgtat
     5761 cttgatttaa atagtaatat gcaaatgatt ctgcaaaaaa ttcttctgca tatacaccta
     5821 agtagtttac attacctaaa gaacgacctt ctttagcaaa tatttgtttg aattgtgctg
     5881 atttagatat atcatttaat actatatggt ctattgcatg tgctgtttca tgtaattcta
     5941 aatttattgc atcatgccct ttacctttgt tactaaatcc tattctaaga gctactgtac
     6001 ttccacctaa acctggaaca tcatcccaag tttttccagt accttcccat cccttaggaa
     6061 ctacaccttt taaataagca tattcttttt catctgttaa ctttccactt aataatttta
     6121 tttttaagtt attttgcttt aatgcattta aatactttcc atctatatta gctaatctat
     6181 ttaccatagc attagcttcg tttttatcgt aatttccagt tggaaaaact actatttgac
     6241 taagtgtgtc tttattttgt tgtatagtag tactatctgc atgtgcactt actggaactg
     6301 aacttattaa gaatattgat attatagcta ttaataattt tttacttggt ctcatattat
     6361 tccctccttt gagttaggcc gggagtaata aatttcaaaa taaaaaagat acccaagagt
     6421 atctttgtat atacaaaaag aaatttttac tcttttcggc aactggctgg catggtttac
     6481 taaactttag cccctatagc tttgcgtgac taggtttccc tagttttgcc tcatttaatt
     6541 ttatatctat attatagctt atatcttttt aattgtctat atccctttgg aacttttttc
     6601 taaaaagtta ttattttttt acataaatct tcaaaaaaaa atatcttcta acaatatttt
     6661 cattattaaa tattgtttta gaagatattt tttaatataa cttatatttt ttataatgtt
     6721 agggtctaat ttgtattttt atttcttctt aatacgataa gtcctacaat agctcctata
     6781 accaaaagta tttctccaat aattgtagta ctatcccctg tttttggtgg tactggtgga
     6841 tttaccaaag tatcatcttt aactttatta tttttttctt ctgacactga tggctttact
     6901 acttctggta ttggaataat ctgtttaaca ggagtttcta tcatctcttc tgatggtggt
     6961 actggcggat tcaatacctc tggtattggc ggatttatta tatcttctga tggtggtatt
     7021 ggtggattta taactatagt atcctctgta tcagatgaac ttgatggttt ccttggttta
     7081 tctgtgcttg gtggtactgg tggatttact attggttcat ctgtatttgg tggtgctggt
     7141 ggatttactg gtggattttc ttgtcttatg ttttctatat ttccaatatc cactattcta
     7201 ccaatatctt tattaccaat ttcaacctta attttatcct tcagtttttc atatccactt
     7261 ggagccatta tctccactaa ataatatgtt cctggttttt ttatactaga ttgaattata
     7321 ccatcttcat cactaactaa atccttaact actatatttc caaatgcatc ttctaaattg
     7381 aacttagcac ctgaaagtac atttttatct aaagcatcca cttttttagc ttcaaaagca
     7441 taaccttgct tagtagtctc tactcctgga ggacttgaac cattaatctg agccacattt
     7501 tgtatagtct ccccaacttt tacatttgtg aagtctgcat caaatgtaac ccaactttgc
     7561 acaggttccc cttcttttat ctgtttaaat tcgtatttat ttactaatct tattgtattt
     7621 tttccatctc cagcttcttt tttaagttca agattatcag aatattttat attagatatt
     7681 tctagagctg agtttacttc atctaaaaga tttacttgtc ctgcatcata aactccataa
     7741 gttgacatat tgattttgta tttaacaatc tggttatcac tactatttaa aactttttta
     7801 tcaacttctt tttttgcgct tattaatcca gaatttttct taaagtttat ctcatcattt
     7861 ttatcaattt cactattatt tgcatcaaga acagctctat ttatatagct atcttcaata
     7921 ctaattacct ttaatgtata agctataaaa aactttgatt ccacttcttc tttactgaga
     7981 ttaattgtaa atccagttat aactttatct acttgcccac tttcatttct tttttcttct
     8041 actttatatg aaatattatt ggcaattttt tctttactcc aaggagtcca gctcccattc
     8101 attggaattt ctacaactct aaaatcatgc ccagcttctc ctgaagtagt taatctcatg
     8161 ccttctggta tattatctgt tataacaaca ttacttattg gtgatttatc atcatttatt
     8221 gaaatctgat agtttattac ttgaccaact tgagtaatat ttgttgctcc tgaatgtcct
     8281 ttagtaagaa gataatcatc tcctctatca aatatactta cagtagcatc atcttcttgt
     8341 atcttcttac catcagattc aatcctagct acattcttat atttattacc atgttttgcg
     8401 tttttgtcac tgattctagc cttatactta acagtatacg atttatctat atttccaaaa
     8461 ttaactgaaa tagaattttt actctgttca atattaaaat cactaacttc ttttccatca
     8521 cttgttacta cacttacaga accatttatc aatgtcaatc cttcaggaag ctcatcatac
     8581 aaaataatat cttttaactc ttcctgcttt tcatttactt gtatactgta atttatatct
     8641 ttatttttaa ctggcataaa tacagtagtt gatttttttc cttcaacaag ctttttaagt
     8701 gttcctttag catctggaac atccgtttta tctccaggtt ttatttctac atctatatct
     8761 ccacttccac tttctatttt tccagtagtt tcaatatgta tttttgcatt agtatcttca
     8821 tcaacctttt taacatatgc ttccaaacct attttacctt gcattacact ttggccatta
     8881 ggtaattctc taaatgtaag tgtatactct ccattacttt ctgttttatc aaagcattca
     8941 cttgtataat ctaaattaac tttattgaat acatctggca ccttaaaagt tattgaatct
     9001 cctgcttgta ttccttttga tgttggagta taagttaaat taaatgatat ttttttcatt
     9061 gattccgttt ctattgtatt gttattaatt tttacaacaa atccatagtc ttcattttct
     9121 tcattaagtt cttcttctaa aactttcaat aaattttcat cttctaatag attatcttcc
     9181 tctggagttt tttctgtatt tttattgtct agtggttttt catcttctaa agcttgttct
     9241 gactttttat tatctgttga cttatcttct tctggtatct gctctggctt tttattatct
     9301 attggctttt ctccttctgg agtttgctct ggctttttat tatctgttga cttatcttct
     9361 tctggggtct gttctggttt tttattatct gttggttttt cttcctctgg ggtctgttct
     9421 ggttttttat tatctgttgc cttatctttc tctggagtct gttctggctt tttattgtcg
     9481 ctttccttct cctctccagt tgatgaatta gttgaaccac tttgaatctg actattctct
     9541 cctaattctg aagcataaga tacagttgga tatattgtag acaccaccat acttaaaatc
     9601 ataattaaac taattaataa tgcctttcta tttcctttct tcatgtaaat ctcacccctt
     9661 taggttacct ataatttata atctaatctt ccattaatat cgtatataaa gtaattttta
     9721 ctaaaaaatt atacaaaaaa agctatgaaa ataaataaat ttatattcat agccggttgc
     9781 accactgact cagtatatcc ataaggtgcc caataataga atatacttca acatctctat
     9841 aatatttcat aaaaacaatt aaaattgctt tatttatatt aaaaatacaa ttatatactt
     9901 ctattatatt atgcctctat actattgtct atccttttta cattttttac cttatgatga
     9961 gattaattaa taattaaata aaatccttat ctacaacaat tacacaatta tattcaggat
    10021 ttatctcctt aactaatttt gttatatctt cttttaagtc atcttctgac atgtttttgg
    10081 aaaattcgga aggattaact actatatcaa atattaaatt cttcttgctt ccttttccaa
    10141 ctattctaaa atcatgcata gattttacaa gactattttt cttaagtatt tcttcaagtt
    10201 cttttttggc tgacataact tctttatctt caagacatat tgggtccatg tgaattacta
    10261 gatatatatc taatttttca gatatttctc tctctgcagt atctataatt tcatgtattt
    10321 ccattatatc tatgttggat ggaatttctg catgtataga tgcaatacat ctaccaactc
    10381 catagttatg aataattaaa tcatgtgtac ctattatata tttataagat aacaatagct
    10441 ctataataga atttaccatt tcttcatctg gtgcttcccc taaaagagga tttatagtat
    10501 cttttacaag agagaaacca gcataaagta tcactaatga tactacgata ccaacatatc
    10561 catctatagg aaaattagta aatctagcta caataaatga tataactaca cagcttgaag
    10621 taaatacatc tcctaaagca tcaagtgaaa ctgcttttaa ggctgaagaa tcaattttat
    10681 ttcccataaa tttattaaaa cgactcaacc agattttaat cattatagat gcaattaaaa
    10741 gtataagtgg tattacttca aattttacag gaattggatt tactatcctt tctatagatg
    10801 actttacaaa ttgaagtcca accaacatca ccataaatgc tactattaat gctgataaat
    10861 actctattct tccatgacca aaagggtgtt ctttatcagc tggcttactt gctaatttaa
    10921 aacctatcat agttattgct gaagatgcca tatcagaaag attattaaat gcatcagcca
    10981 ttatagctat actagatgtg agcattccta tgaatacctt tatcacaaat aaaagtaaat
    11041 tggataatat acctactaca ccagctacat aaccatactt atttctaaca tctgtatttt
    11101 gaacgttttc gctatctctg ataaatgttt tgactaggat tttggaaaac atgaaactta
    11161 cctccctgcc ctttatttta ttaacaaatt ttataatcta tatctttatt tatgataaca
    11221 taatttatat ataaaattct taaaaatcac tctacttcag taaaatatta ttctatatat
    11281 ctatttagat acgaatttta tatttagtaa ag
//
LOCUS       DACULQ010000126        11156 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.127, whole genome shotgun sequence.
ACCESSION   DACULQ010000126 DACULQ010000000
VERSION     DACULQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.127"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            328..1218
                     /locus_tag="JA154_13510"
     CDS             328..1218
                     /locus_tag="JA154_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996862.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(1611..2567)
                     /locus_tag="JA154_13515"
     CDS             complement(1611..2567)
                     /locus_tag="JA154_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT7996863.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3270..4265
                     /locus_tag="JA154_13520"
     CDS             3270..4265
                     /locus_tag="JA154_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter ATP-binding protein"
                     /protein_id="HAT7996864.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNYFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4288..5106
                     /locus_tag="JA154_13525"
     CDS             4288..5106
                     /locus_tag="JA154_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996865.1"
                     /translation="MFYKFKQSIRRYYEVAKINFKVQLIWRFDVVVNMVFTIVKILFA
                     YILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSEEICNRVKLGTFSKYM
                     IIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFTSNPVQILISILMIFL
                     GFIFMMKLHFFIGILSFKFLEISFLKMIIENIVEFITGTMIPLVLLPETIVTVMKVFP
                     FYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYKRMRTLFDGVGI"
     gene            5118..5897
                     /locus_tag="JA154_13530"
     CDS             5118..5897
                     /locus_tag="JA154_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996866.1"
                     /translation="MKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLMFNSIY
                     SHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYITKPLDP
                     LFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLLFYDVC
                     IILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGIFKVLFYLILPYGIMSTIPTQ
                     ILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6182..7561
                     /locus_tag="JA154_13535"
     CDS             6182..7561
                     /locus_tag="JA154_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT7996867.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIF
                     KYKNRYKYM"
     gene            8009..8722
                     /locus_tag="JA154_13540"
     CDS             8009..8722
                     /locus_tag="JA154_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996868.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(8994..10532)
                     /locus_tag="JA154_13545"
     CDS             complement(8994..10532)
                     /locus_tag="JA154_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996869.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVREL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         3990 a         1300 c         1697 g         4169 t
ORIGIN      
        1 tttataaatt taacacaaga agaaataatg aaacttgagt cgagaataga aaagttagat
       61 aaaaatgctg aagaatacaa acaaaattca aagactaata tatttgataa aacagatgaa
      121 caaaaaaaac atataaatgt actgatttaa acaatatgtt ttaaaaaaat aagtttatta
      181 tataatgaaa atataaaggt acaatttcaa tacacttcta ttaaaccaat aatttatgaa
      241 tcagatttaa tttcaattta ctaataacca tatatttatg agttaataat tttatgtttt
      301 aaaaagaatt aaataagggg gagtagtatg aagaagaata tattaactac tatgattacc
      361 atgattatta tattgatgat gagtatgaca gtaatggcag agccatatca agaagatgaa
      421 acatctagct tgaaaaaaca gagtgcaaat gaacgtaatc agactaatat aggcacaatg
      481 gatggcagag tgcaaacaaa tgcaataagt aaaggtgcta attatgaccc taaatatcct
      541 cttaaaggaa tgttaacaca acttggtctt aatacaacag gtaaagaggg atgggattta
      601 ctctatggag gaaatcccaa tggagttgag cttcgagcag atggtaaata ttattatata
      661 aataagggta tggaagggct aggattttat gcggcagcat tgtcaggtgg ctcatatcag
      721 agaataggct ttggaaaact tgaccctata gaagtaatag ctgatggaaa ttggatttct
      781 tacaaacaag ctcaagatac acttacagta atcaggaatt ttttaaatag ctttgactgg
      841 agaaatgcat ctgagatgga aagagctaat agagctgcaa aattggtaac cgaagcaaaa
      901 tatgcagact ctaaatattg taacatagtc tatggtaatc tggttgataa acgtggtgta
      961 tgtggaagtt ttgcaagtag ctttcattta ttaacaaggt taatgggaat ggattcacta
     1021 tctatattaa atccatcatt aaatcatgct tggaactata tacaaattga tgggaaatgg
     1081 tataggtttg atgggtctga aatatcagct tttggtggat cattagactt tgattaccgt
     1141 aaattaaagg atgcaacaag agagatgact acttactatg atgctaaggc attgtctata
     1201 ttaggattta atcaataaaa atagggggag gcttcttata gagccttttt ttattgattt
     1261 gaaatttaat aaggaatttt atatgaaaat ttaatcaata aaagcaaaaa aggctcttta
     1321 taaagccttt ctaagtgttt ttttaagtaa aattttttat atcaacatca gttttaatca
     1381 aaacatataa cattttggac ttacttttaa aaataaaatt attaaaatat aatattaatc
     1441 atgtttttag tttattatat tctataatag tggtcatgtt gactattgta gttttattaa
     1501 gaatagttgg tttatataaa tatatgcaaa gatagcttgt tgtttacttt acttattagt
     1561 atataggaca agatactcta ataaatttgc ctattttaat atcttttgta ctaaaaccaa
     1621 ctaaagtttc ctttattatt tcgttttgaa taatacaaag cataatccga ttgtttgtat
     1681 aaagactgga aagtatcaga gttattttct aatttagata tacctataga acaagaatta
     1741 ctctctaggc ataattctaa aaccttcttt gccttatttt caacattctc ttttgattga
     1801 ttactatatt caaaaacagc aaattcatct ccaccaagtc ttccaataat agcatcattt
     1861 acgaatactt tagtcaaaat atttgaaaaa tgtattagta tttcatctcc tcgaacatga
     1921 ccaaagatat catttacctg tttaaaatta tctaaatcaa agatgtaaaa gtatccatgt
     1981 gaaacagttt taagcatttc ttttatgtgt acttcaaaaa aatgtctatt gtatagtcca
     2041 gtcataaggt ctttttctga ttttagttta aattcatttg ccattgctag tgcttcttgt
     2101 gctttttcag ttattgtttt agggtaatac tcatctttca tttggtcaaa gtttggaata
     2161 gaatggtgaa catggataag tttaatcata tcatctgtaa ctctgtatac catagtaaat
     2221 cgagtatcca tttctacata gatatctttt tctaggtaat ctttttctat tacccaaaga
     2281 gtaccatata ctataaaagc ttcgtctgta actttggtac atgaatacca ctcatctata
     2341 atctcaaagt ctatttgctc tgattctgtt tggtcttttt caagacctat aataaattct
     2401 tctaatgttt cgtaaaattc atgttttcct gttccaatta atgcaaggtt gtcatctaat
     2461 aaggaaataa taaatttaag gttttcttct tctggattta aaatataaat atgccaaatt
     2521 ttttttgata aatctcttaa ttgtttttgt attgtattat tttccataga ttaccccctt
     2581 aagatttaca gtttatattt taatgtatta taagttgaac tcttattaca atattattat
     2641 aatatagaac taaagaaatt acatcctgta taagtaaaag tatatctttt ataataaaaa
     2701 tattacagta aaataaccag ttttaatata cgtattatat ttttaattta taaacgaata
     2761 ttataaatga aatttaaatt caaccctgaa gaaataagct aaatgtatta gtatataaat
     2821 ccatacttga acataaataa cctttatgat atgatatcaa taataatatt atgagaggag
     2881 tgccttgatg tcggagttat ataatggtaa ccaacaatta aatagttgga gacacatata
     2941 ttctgtataa tcagaaattt caagtgtatt tttaatacat ttaatttttg tgtcttattc
     3001 tgcctttata gataactgac atatgacaac aaatagtatt taaaatatat attactttgt
     3061 taaattatgg tgtagtaggc tttaatctgc tacactttta ttaattttgt atagtattat
     3121 tttgataact attttgaaat agagagcatt aatttttcta gtccacagga gttttctgtt
     3181 tttgtgctgt taaatataag cacagagaaa ggcagatata tacctgtgga ctttttttat
     3241 ataaattatg aaatatggag gtagttaaaa tgagtataat tgaacttaat gaactgacta
     3301 aatcttatag aagatttgag aaaaaggcag gatttatggg gagtgtaaag tctttattta
     3361 ataggaaata tacagaaaaa acagctatat cgtcatttga tttaaagata gaagaaggag
     3421 aatttgttgg attaataggt caaaatggag ctggcaaaac tactcttatt aagatgttaa
     3481 ctggtattat tgcaccgtct tctggagaaa tatctgtaat gggttattac cctaacaaat
     3541 tagaaaataa ttttaaaaaa caatatgctg ttgttatggg ccaaaaaagt caactattct
     3601 ttgaattgac accagcagat acgtttcttt tatttaaaga attatatgat atatctaatg
     3661 aagaatataa gaaaaacttg gattacttta tcgaattatt tgaggtatct gactatttaa
     3721 atgttcaagt taggacgctt tctttaggag aaagaatgaa aatggaacta atagtagccc
     3781 ttctacataa tccaaaaata ttatttttgg atgaaccaac tataggctta gatgctatag
     3841 cccaaaaaca aattaggtat ttcctaaaag aagtaaatga aacaaaagga actactatta
     3901 tacttacttc tcattacatg gaagatatta agagtctctg tagtagaagt attgttgtag
     3961 aaaatagccg taaaatatat gatggttcta cagagaaact atttaattat tttcaaaaat
     4021 ataaaaaaat aacagttagt ttttccaatg aaactaacat tgtatttcca aaagattgta
     4081 aagttgttga acaaacaaat cataagatat cagtaattgt gccaaaagaa agaggaaatg
     4141 aaacgattaa atggattttt gataaatatg atgttaaaga tgtaatgatt gaagaagaag
     4201 atataggtag tattgtagag aggatatata agaaaggaag tgatgagtat tatgagcaac
     4261 tataatgaag caagttcaaa tgattcaatg ttttataaat ttaaacagag tattagaagg
     4321 tattatgaag tagccaaaat taactttaaa gtgcaactta tctggagatt tgatgtggtg
     4381 gttaatatgg tgtttacgat tgtaaaaatt ttatttgctt atattttatg gaaagcaatt
     4441 tttggtgaaa ataaaatagt ctctggtttt acatttaact ctatgttatc ttactatata
     4501 ataagctcat ttttagcagg tattgatatg tcaaagagta ccagtgaaga gatatgtaat
     4561 cgtgtaaaat tgggcacctt ttcaaagtac atgattattc caattaatgt tcaaaattat
     4621 tttattgcta aaaattttgg aatgacatgt ttttattttg tatttaatat gatttctgca
     4681 tttgtatgga tttttatatt taaaatagat tttgccttta catcaaatcc agttcaaata
     4741 ttaatttcaa ttttgatgat ttttctaggg tttattttta tgatgaaact ccacttcttt
     4801 attgggattt tgtcatttaa atttcttgag attagttttt taaaaatgat aattgaaaat
     4861 attgttgaat ttattacagg aacaatgata ccacttgtac tcttaccaga aactatagta
     4921 acagttatga aggtatttcc attttattat acaacatatt tgccatctat gttactaata
     4981 ggtagaaaca gcaatgaggc aatacaagga ttaattattc tagcattatg ggttttattt
     5041 ttttctgtag taaataaagt tatgtataaa agaatgagaa cattatttga tggggtgggt
     5101 atatgatgaa aaaaaatttg aaaataatat ttatgttatt aaaaatgaaa ttatcaaaga
     5161 tgatggtatt tagattcgat ttttttggag cattttttgt agatagttca ttatttatat
     5221 tacaacttct aatgtttaat agtatatatt ctcatgtaga ctcaataggg ggatggcaac
     5281 aaggagagat gttaatattt ataggaactt tctcattaat aaatgcgata aatatgacga
     5341 ttttcttttt tggtatttat gatattccac gaaaaatcca agaaggagaa ttagactact
     5401 atattacaaa accattagac ccactattta ggttaacatt tgaaaatatt aatattggtt
     5461 catcaccatt aattctagca agtatactta ttgttttata tggaatatct gagttaaact
     5521 cagagataac tttagttatg ataataatat atattttcat ggtgttattg atgacattac
     5581 ttttttatga tgtatgtatc atacttagaa cagtttcatt ttttgttata tcatcttctg
     5641 cgataatgag attagaagat aatttattgc caatgaatat gaaaattcct ggtgtaatat
     5701 atgaaggtat ttttaaagta ttattctatt taatcttacc atatggaatt atgtcgacaa
     5761 ttccgactca aattttggct gggacaataa caattaatgg attaatatac tcaattttaa
     5821 ttgtattttt gtttacattg tttatgtgga gattttggaa gttaggtatg agacattata
     5881 aaagtgcaag tagttaataa attagtaaaa atagacaatg tataactata tataaatacg
     5941 tttcatatga cactaaacta tgtgaacaag tatttgcgct aaaggcactg aaagctataa
     6001 aatgattaaa gtaaggattt aaaagtttct ttagtagaaa tataattgtg taaaaaagca
     6061 agattaatta aaactatatc caaataatta gatataattc ttatgcagaa aggaggtctg
     6121 taaatgtcta attgtttgga atatatttta aaaataatta atgtacagaa aaataaacaa
     6181 aatgagtgat gtatattatt cagaaaatca tgaacaagat aatctttatg agcaagataa
     6241 tttactggca caaagattta atttatttac tctattgaag tttgcatttc ctactatttt
     6301 tatgatgatt tttatggggt tgtatacaat cgtagatact atatttgtat caagatttat
     6361 taatacaaat gctctatcag caattaatat tgtatgtcca attattaatc ttatagttgg
     6421 aattggaact atgcttgcca ctggaggaag tgctattgta gcacgtaaga tgggaaatgg
     6481 tgatattgag ggagcaaata aagctttttc tttaatcgta ttatcaggag ttgtaattgg
     6541 ggtaataatt actggtatag gtattgtatt tatggattat atcattatat ggttaggagc
     6601 aagtgaagtt ttaattaaat attgtcgttc atatcttttt attatcttgg tttttgcttc
     6661 tgcaaatata ctacaagtat tatttcaatg tttatttgta acagctggaa agcctcaatt
     6721 tggattatgg ctagtgttag gtgctgggtg tgctaatata attcttgact atgtgttcat
     6781 tgttctgtta caaatgggta ttagtggagc agcacttgca actggagtag gatacctaat
     6841 tcctaccatt tcaggtatgg ttttcttttc aaaaaataaa gggttactta aattttgtat
     6901 acctagacta gataaaagaa tattatttga agcttgtttt aatggttcat ctgaaatggt
     6961 gggtcaacta tcaacagcag ttactacttt tttatttaat gctatgatga tgaaattaat
     7021 tggtgaagat ggggttgcag ctattacaat aattatttat tctcaatttc ttttaacttc
     7081 gctttatata ggtttctcaa tgggtgttgc accaataata agttataact atggaagtga
     7141 aaataatacg cagttaaatc atgtttttaa gatttgtata ttatttattt cagtagcatc
     7201 tttattggta tttatctttt ctatatcatg ttcaccaatc attgtagaga ttttttctca
     7261 aagaggaagc cgagtttatg aaattgcaag gtccggattt tatatatttt cttttagttt
     7321 tattttttgt ggatataata tttttacctc atctatgttt acagcattat caaatggaaa
     7381 ggtgtctgca atattatctt ttctacgtac atttggattt ataactatag gtttattgat
     7441 attgccaaat atattaaata taaatgggat atggttagca gtaccaagtg ctgagatttt
     7501 aaccttattt ttaactgttt tttgtatatt taagtataag aatagatata aatatatgta
     7561 gatattaaat aaatagctta gatagatatt ttatgtttta ataagtaatt caattagtgg
     7621 tagatatata ctattgaaaa ttaaagttat aattgatata aagaattaat aaaaataaca
     7681 tataaaagtt taaaagggag ttgaacttaa agtttgatta gaaaaagtca attttaagac
     7741 aactcctttt attttactat tgtattaatc tgtatctgta agtgtattag tctaatatta
     7801 taaactttgg atgaaattaa ttttctatag gttttaatgt atcatacact ttattttaaa
     7861 gaaacttatc ttaattttca tagtgattga atttgtctat tagagatgtt actattagta
     7921 atgtaaaagc aagagtagtg aatataaaaa acaagatagt gatactgcaa gattagaata
     7981 taataaattt ttatggagag atttcaatat ggatacatgt tttaataaaa aaatattact
     8041 tgttgatgat gaaaaggata tagtagattt aattgaagaa gtactcataa atgatggttt
     8101 taaaaacatt attaaagctt ataatggtct agatgctatt tcgctgtgta agatagcatg
     8161 tccagatgtt gtcattcttg atataatgtt accagatata gatggtatag aagtgtgtaa
     8221 aaaaattcga gagttttcat actgttctat tcttttttta tcatcaaaaa atgatgatat
     8281 tgacaaaata ctaggtctta gtagtggggg agatgactat ataacaaagc cattcagtcc
     8341 aagagaaatt gcttttcgag ttaaagcgca attacgtaga caacagtatc aaagcattgt
     8401 accatcagat agtgaagtta ttaaaatagg agatattact attgatatag aaggaaaccg
     8461 tgtatataaa gatagaaatg agattgagtt aactggaagg gagtatcatc ttttaagcta
     8521 catggctcaa aatgtaaata aaattatagg aaaagaaaga ctctatgaac aagtatgggg
     8581 agtatatagt agtatttgtg acaatacaat tatggtgcat atacgacata taagagaaaa
     8641 aattgaagat aacccctcaa atcctaaaat acttataaca gttaaaggac taggatataa
     8701 acttgtgaat agaatcgatt agaatagatt ttaatattaa tgaagatatc tcaagttgaa
     8761 tttttagtta atgaggcatt cttttatggg tgaaatcaaa tgtattttcg ttatctcaac
     8821 aatgaagaag aggtctatct ctattttgag atagcctcat taagtttata tcctggtaat
     8881 gtactttatg ttctaataaa atttttttag tttatttgca gtgaaacatt aactagaaca
     8941 ttaaaatttg cattgaaata ccaattaaat aatattgtat attgtgtata aacttatact
     9001 aattgttttt ctatattaaa tactttatca atccaacctt tataaaattt ttcttcatgg
     9061 gaaactagga ttatacttcc tctaaactgt ttcaaagcat tttgtaaagc atcctttgtc
     9121 tctgcatcaa agtgattggt tggttcatcc agaataagaa aattacaagg agataacatc
     9181 atacaacata atttaacctt agattgttca ccaccactta aagtagatac actccttgaa
     9241 acatgctctt ccttcacacc acatctggct agatgacgtc taatctcttt tgtatttaat
     9301 tttggatatt tatctgctac tatttgaagt ggggttttat caggattttc ccattttaaa
     9361 tcttgctcat aataaccaat tttaacttgt tctgaaaact ggaaatctcc agatatacga
     9421 ggaatatctt tgactaatgt ctttaacaag gtagatttac cgattccatt aaaaccagtt
     9481 ataactattt tttgacctcc atcaacagag aaatttaatt ttggaagtaa agaatagtag
     9541 tatccaactt caagatttgt tatagtaaga gcattttgag ccgatatttc aatttctgaa
     9601 aattgaatat ttggttttcc tgtaaaacta ggaggagcta ttctttctat tttatccaat
     9661 tgtttttgtc ttcctcttgc aatttttgaa tttactccag ctttattttt acgtataaat
     9721 gtttcttctt tttctatttt cttttgttgt gcttgatacc ttctaatata atcttctctt
     9781 aaatgtgatt tttgtttaag gaactctgaa tattttccat gatatttctt aatcatgcca
     9841 aattctatat caagaatacc tgtagaaatt ttttctaaaa agtcaaaatc gtgggaaact
     9901 acaataaatg ccccattaaa tgtatttaaa taatttgaga gccattcaac atgctcttta
     9961 tctaaaaagt ttgttggttc gtctaataat agaacatcat ggttagaaag tagtaatttt
    10021 gctaaaatta cctttgccct ttgaccacca ctaagctctc taacaacacg attcattcct
    10081 attgaatcta atccaagacc atttgctatt ttatttattt cattatctat agaatagaaa
    10141 ttacttgcta ttagttgttc ctgataatct gatgctttga ttagatattg ctcattttca
    10201 gatatagcac ttttttgata gagtaaattc atttcctttt ctattttata aagttcttca
    10261 aatgcagtgt gtaaatagag tgatattgtt gtatctctat tgatttctgc atattggtct
    10321 aaatggccta tattgatatt aggttgccac ttgatttctc ctgaatctgg aacaacttct
    10381 cccaataaaa ttttaattaa tgtactttta cctgtaccat tttggccaac tacacctata
    10441 tgttcacctt tgaataaatc aaagttagct ttctcataca gtgatttatc cataaaacat
    10501 tgtgataaat tagttacttt taataaactc ataattcaaa ctccttttct tttattaaaa
    10561 aatttatgga aataaaaaaa gcagaggaat ggcattgtaa aatgccgacc tctgcttttg
    10621 gaaaaacaaa aggtcataga caagcattgt tgtctatgac cagaaaatct atattatcta
    10681 tcggcaaata ggtaatttta aataagcata gccaaaaaac tatactaaac ctatatttgc
    10741 cgatgaagat agaaagcttt aagctttcag attaacctct acacaacgct tcatcgtata
    10801 catttgtgta gagttaagtt ttctaacaag tttaactaat tctatataaa tcataattat
    10861 tcacatccta atcttagtat taattctagt ttatctgtat ttaaattgta cttttgtact
    10921 ttgtggatta tcttatttat ttaaaatatg ttttattaat tatagcttaa gttatgttat
    10981 cataatactt agttatagtc aataagaaag ttatatttac tattattata ttaatttagt
    11041 aaatctatca ttacaaagtt atttgaagac tcgctatcaa taaaatttta tagtctataa
    11101 gaaaattaag attatgagtt ttataatttt gatataggga tactattttc ataaat
//
LOCUS       DACULQ010000127        10850 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.128, whole genome shotgun sequence.
ACCESSION   DACULQ010000127 DACULQ010000000
VERSION     DACULQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.128"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1173)
                     /locus_tag="JA154_13550"
     CDS             complement(<1..1173)
                     /locus_tag="JA154_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HAT7996870.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMG"
     gene            complement(1196..1861)
                     /locus_tag="JA154_13555"
     CDS             complement(1196..1861)
                     /locus_tag="JA154_13555"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088202.1"
                     /note="catalyzes the formation of riboflavin from
                     6,7-dimethyl-8-(1-D-ribityl)lumazine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAT7996871.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(1895..3007)
                     /locus_tag="JA154_13560"
     CDS             complement(1895..3007)
                     /locus_tag="JA154_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
                     /protein_id="HAT7996872.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3139..3260)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3449..5931
                     /locus_tag="JA154_13565"
                     /pseudo
     CDS             3449..5931
                     /locus_tag="JA154_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088204.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
     regulatory      6020..6131
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6273..7574
                     /locus_tag="JA154_13570"
     CDS             6273..7574
                     /locus_tag="JA154_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088205.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiC"
                     /protein_id="HAT7996873.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            7628..7822
                     /locus_tag="JA154_13575"
     CDS             7628..7822
                     /locus_tag="JA154_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiS"
                     /protein_id="HAT7996874.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            7815..8618
                     /locus_tag="JA154_13580"
     CDS             7815..8618
                     /locus_tag="JA154_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088207.1"
                     /note="catalyzes the adenylation of ThiS which is involved
                     in the formation of 5-methyl-4-(beta-hydroxyethyl)thiazole
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HAT7996875.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            8681..9451
                     /locus_tag="JA154_13585"
     CDS             8681..9451
                     /locus_tag="JA154_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAT7996876.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            9468..10571
                     /locus_tag="JA154_13590"
     CDS             9468..10571
                     /locus_tag="JA154_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAT7996877.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            10572..>10850
                     /locus_tag="JA154_13595"
     CDS             10572..>10850
                     /locus_tag="JA154_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAT7996878.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFF"
BASE COUNT         4115 a         1303 c         1842 g         3590 t
ORIGIN      
        1 tcccatttta tcttttttag tttttaaata tagcttatta aagtcattaa tttcttcatt
       61 aatagggatt ctattttcga tttttattcc atatttctct aattgattta ttttatctgg
      121 gttattactt aaaagattta tactttttat gtttaaattt tttaatattt gagcagccat
      181 atagaaatct ctcatatctt cttcaaatcc aagcattaaa tttgcttcaa cagtatcgta
      241 accttcttct tgaagtttat aagccttaat tttatttaaa agaccaatac ctctaccttc
      301 ttgcttcata taaattaaaa ctccacttcc attttccaca attttcttca tagaagattc
      361 tagctgtaat ccacaatcac attttagaga gtggaacaca tctcctgtta aacattcaga
      421 atgaagtcta actagagtat tttctatatt tatatttcca taagtaaggg caatgtgttc
      481 ttcatttgaa taagtatctc tatatcctat gatttcaaaa tttccatatt ttgtcggaag
      541 gaaagcacta gatacttttt ctatagtagt ttcatttatt ttgcggtatt ctataaggtc
      601 ttttatagtg atgattttta aattatgctt ttctttgaaa atcattaaat catcaactct
      661 tgccattgta ccatcatctt tcattatttc acagataaga ccaatctctt taaaacctgc
      721 aagtctagca aggtctactg aggcttctgt atgtccatcc ctgacaagaa ctccaccagt
      781 ttttgctatt aaaggaaaga tgtgaccagg cgttttgaaa tctttacttg tagaattttc
      841 atcacagaat aattttacag tttcagctct ttcatatgca gatataccag ttagagtatc
      901 tgctccatct atagtaacag taaaggctgt ttgaaatgta tctgtatttt ctctaaccat
      961 aagaggtata ttaagacttt taaatttttt ttcagtggca ggtagacaaa ttagacctct
     1021 accatatgta gccataaaat ttattgattc aggagtagcc atttcagctg ccatcaataa
     1081 atctccttca ttctctctac tttcatcatc aactacgatt accattttcc cattttttat
     1141 atcttcaata gcttcttcaa tagtattaaa catgtaattc actcctttat tatttttaaa
     1201 aaccattttt aaataaaaaa tctttatcta tatttgattt atggttacta ctattagaat
     1261 ttaattcctt gtagttatta gccataaaat tattaacata ctttccaatt acatcgcttt
     1321 ctaaattaac gaaatctcca acattctttg tcaagagaat agtgttaatt ttagtatgag
     1381 gtataataga aaccttaaat atttcatcat ctaaatatga tatagttaaa cttacaccat
     1441 caatagcaac agaacctttg agtatcattg aggataaaag ttttttatca ggtcgtatac
     1501 ttactaaaac tgcattttca tctttttcaa atttgcagat agttccttta ccatcaacat
     1561 gacctgttac tatatgacca ccaaatcttg aagaaagact catagctctt tcaagattta
     1621 ccttgtcatt tgcatttaat aaacctaaat ttgtggaacg aattgtctcc atcataacat
     1681 cggctgtaaa ttcatttttt ccgaggtttg aaacagttag acaaactcca tttacggcta
     1741 tactatctcc aagatttgtg ccatctaata ttttatttga tagtatggtt acttttccac
     1801 tcttaccatt agcagtaatt tttctaagta ttccaacttc ttctactatg ccagtaaaca
     1861 tcctaacctc ctatatgaat gtattaacaa aaatttaatt taaaacatct gattctataa
     1921 gaatatcgcc atctatcaaa gttacattat tgattgttaa ctgtgttgaa tctgacaaat
     1981 gatttattcc ttctccagaa acgaatgtag gtgcatcttt accacctata atcttgggtg
     2041 atagaaataa tttgacttta tcaactaact tatttctaaa gagactgtca ttgagagtag
     2101 caccaccttc aactagaaca gaatcaatgt ttaactctcc taatttatac attacaaact
     2161 ccaaatctac taagttgttt tttgaaggag aaattattgt ttcaacatta ttttctttta
     2221 acatagataa ttttatactt tctttgcaac atgtgaaaac tatagtttta ctggttttat
     2281 ctttaaccaa attactatta agtggcaact ttaggtgtgt atctattact attcttgtag
     2341 gatgagacac tttttcttga ttgagcctac aagttagtaa aggattatcg ttcaaaactg
     2401 tatttatgcc aaccattata ctttggtatt tatttctgta tttatgagtt agataccttg
     2461 atgattcatt agatatccat tttgattcta gtgattttgt ggcaatttta ccatctagac
     2521 ttactgctga ttttacaata caaagaggtc ttttgttttt tatatgataa aaaaatgcct
     2581 catttaaatc tcttgcttca tcttctaata ttcccacagt aacatcaata ttgttatctt
     2641 ttaatttttt tactccatta ccacaaacaa gtggatttgg gtctaatgta gatattacaa
     2701 ctctcttaat cttattttgt ataattttat ctacacaagg aggcgttttc ccataatgag
     2761 aacatggttc taagtttaca taaagagtag agtcttttag actttgttta gcactgttta
     2821 tagcatttac ttcagcatga ttagagccaa atttctcatg atatcctttg cctattatat
     2881 tactatcttt aacaattaca catccaacta atggatttgg atttacaaag ccttctccat
     2941 ttttagccaa ttcaatagct tttttcatat aataaatatc tttttctttt tgattcacaa
     3001 aattcacaat atgtacacct cctgtttaat tggataattt ttaaacaaaa aaccctgaga
     3061 attaactcag ggtttagaat aatctacaat aaatgtttaa taaatgtaga acaaaattgc
     3121 tctacaaaaa cactcattaa atatcttctt ccatccagac tttactgtcg gctttggaat
     3181 ttaaccaaat caaccaagtt ttacattggc tcgcgggcta taccgccgat tgggaatttc
     3241 accctacccc gaagattaag tattaaattt atatttagaa tgtatcataa attattttac
     3301 tttgcaaact atttttttaa aaaatatatg ttaatatata atatagaatg tctataaact
     3361 ttgaaatacc acgattgtga atataaaaat aaaaatttga acataatcag gaaagaagtc
     3421 taattaattt aaattaggag agtaatatat gaataaaaaa cttttagaaa tactatcaaa
     3481 atactatgga tatacaagtt ttagaaaagg tcaggaatct ataataaatt ctatcttaag
     3541 cgaaaaagat gtgctagcta ttatgccaac aggaggggga aaatcaatat gttatcaaat
     3601 tccagctttg attttagatg gaatgacaat agtaatttct ccacttatat cacttatgaa
     3661 agaccaagta gatgccttaa agactatggg aattagtgct gcttttataa atagttcatt
     3721 aagtagcaaa gaatttaatg aaatattaaa taatattaga aaaaataatt ataaaatact
     3781 ctatattgct ccagagagat tagatgcaca agagtttatt gagctaataa ataataacaa
     3841 tgtaagtcaa gttgcgatag atgaagcaca ttgtgtatct caatgggggc atgattttag
     3901 gttgagttat agaaggattt ctgattttat aaaaaatttg cctaaaagac ctatagttac
     3961 agcatttact gcaacagctt cagaagaggt aagaactgat ataataaatt tactgtgttt
     4021 agaaaatcca gattattata taactggatt tgatagagag aatttatcaa taaatatagt
     4081 aaaatcatct agtaaaaata agtatatttt agattatatt caaaatcata aaaatgagtc
     4141 tggaattata tatgttgcaa ctagaaagga agttgaaaat atatataatg gtttatctaa
     4201 gagaaatata tctgtatcaa aatatcatgc aggtttatct aaaaatgaaa gaagtaaaaa
     4261 tcaagaagat tttataaatg ataatgtgga tataatggta gcaacaaatg catttggaat
     4321 gggtattgat aaacctaata taagatgggt tatacattac aatatgcctc aaagtataga
     4381 aaactactat caagaaatag gtagagctgg tagggatgga gaaaaaagtg agtgtgtact
     4441 tttattttct ccaggagatg tacatataca aaaatattta attgatattg gagttgaaaa
     4501 tcctgaaaga aaattatttc agcataagaa gcttcaatac atgattgact tagtttacag
     4561 taattcatgc tatagaaaga caatattgag ttattttgga gaaaattata tagacaattg
     4621 caataattgt agcaattgtt tagtagaggg tgaaatagta gataaaacta tagatgcaca
     4681 aaaagtcatt tcatgtgtag ctagaatgaa aagaagttat ggtgttacta ctattgtaga
     4741 tgtgcttaga ggttctaaga ataaaaagat tttacagctt ggattaaata ctttgtctac
     4801 atataacatt atgagagatt attctagtga agacttaaag aattttgtaa atacattggt
     4861 ttctcatggt tttttagatt tagttgagac tctaggcaat ggaaatagag gtagttttcc
     4921 tactataaga ttaaatgaga tgtctatgaa agttttaaaa ggtgaagtga aagtagagtt
     4981 taaagaaatt gttatgacaa aatcattgga agtagaggat gagttatata atgctttaag
     5041 agaacttaga cattcaattg caagtgaaga aaggatagct ccatatatgg tatttggaga
     5101 tggtacactt agagccatgt ctagtagtta tccaattaat aaagaagaaa tgctagatat
     5161 ctctggagta ggagaaatta aataccaaaa atatggtaga gattttgaag cagtaattaa
     5221 agaatatgtc gaaaaaataa tataaataaa aaaatagatg aaaaaaatgt cgattcaaag
     5281 aacgttaata gtgatttctt agaggttaat acagataaag ttttatatga aaaactgtta
     5341 gtcattagag aagaatatgc aacaaaggaa aaattacctg catatatgat tttagccaat
     5401 aaggctttga aagaaataag tggaagatat ccacttgatg aagaacaatt aaaagatatt
     5461 tctggcatag gtggagtaaa atttgaaaaa tatggagcta gtattttggc agaggttaat
     5521 aaatatgtaa aagaaaataa tattaatgtt atttgggaga aaaagggcaa aaggaagtta
     5581 atattagatg gagaaagtag aaaaaataat gaaatagcat tagatttgct aaatcaagga
     5641 aaagatatac aaagtatcag tcaagaaata gaagtttcac tgtcaaccat tattggatat
     5701 gtgcatgact atgtaaagga tggacatagt atatcatttg aattaggact agaaaatttt
     5761 tataatgaag ataaaaagaa gatgatttta gaagtttgcg aaaaagttgg ctatgataat
     5821 ttaaataaca taaaaagaaa acttccagat tcaataaagt atgaaaatat aagagctgtg
     5881 atacttgact cttatcttga aaacttaaaa aataatatta tagcagagta atttttaact
     5941 taataaaaaa taaaatttgt attattgtgt tgaactaata attttgctgt gttatactga
     6001 atttgtaaaa ataatgaaaa attagagcta ggggcgctag aattggctag ctgagagata
     6061 aagccgtaac ttacaaaccc taataacctg atcaagataa tgcttgcgaa gggaagcttt
     6121 ttaatataat tttattatat aaataggcaa attattcaat gttaggagaa atttgtctaa
     6181 gtgtatatac gattataaga gtgctttcca aacgaggaaa agcacttttt ttattttcaa
     6241 aaactataac tttaatcttg gaggattgta atatgaacta tacaacacaa atggatgctg
     6301 ctagaaaagg cataataact aaagaaatgg aaatcgtttc tcaaaaagaa caggtagatg
     6361 taaatgaatt aagagaatta atagcaaatg gacaagttgt tataccagct aataaaaatc
     6421 ataaatcttt aagtgcagaa ggtgtaggta aaaatttaag aacaaaaata aatgtaaatc
     6481 taggaatttc aagagattgt aaggatatag aaaaagaact tgaaaaagtt agagtagcaa
     6541 tagacatgaa agctgaagct ataatggatt taagtaatta tggcaaaact agagaattta
     6601 gagaaaaggt tgttgaaatg tcacctgcaa tgataggttc agtacctatg tatgatgcag
     6661 taggatattt ggaaaaagaa ttaaaagata taacagaaga agagttttta aatgtaataa
     6721 gacaacatgc tattgatgga gtagacttta ttactataca tgcaggtctt acaagaagtg
     6781 tttgccaaaa aataaaaaat catgagagat tgactcatat agtatctcgt ggaggttctt
     6841 tattgtttgc atggatggaa ttaaataata aggaaaatcc tatttatact aattttgata
     6901 aaatattgga tatatgtgaa gaatatgatg ttacattgag tttaggagat gcttgtagac
     6961 caggttgtat aaaagattct actgatggtg ttcaaattca agaacttgta gtattaggtg
     7021 aacttactaa aagagcatgg gaaagaaatg tacaagtaat gatagaaggt cctggacaca
     7081 tggctattga tgaaatagaa gcaaatgtag tattagaaaa gagattatgc catggagcac
     7141 ctttttatgt tcttggacca ttggttacag atatagcacc aggttatgac catataacaa
     7201 gtgcaatagg aggagcttta gcatgtgcta aaggagtaga ttttttatgt tatgtaactc
     7261 cagcagaaca tttaagattg cctaatttag atgatatgaa agagggtata atagctgcaa
     7321 agatagctgc tcatgcagga gatatagcta aaaatgttaa aggtgctcgt gaatgggata
     7381 ataagatgag taaagctaga gctgatttag actggtgtga gatgtttaga cttgcaatag
     7441 accctgaaaa agctaagaga tacagagatg aatcaactcc tactcatgag gatagttgta
     7501 ctatgtgtgg aaaaatgtgc tcaatgagaa cagttaagaa aatattaaac aatgaggaac
     7561 ttaatctaat ataatttttg gctagtatga ctggaataaa aataagctga aaagaggagg
     7621 tgatttagtg aaggttaatg ggaaggaaat tgaatttgaa aaagatttga ctgttataga
     7681 tttattaaat aaatataatt taaaaagtga tagagttgta gttgaagtga atttagagat
     7741 aattgaagaa tctaactaca atacatatgt attaaaagac gaggacattg tagagcttat
     7801 tagctttatt ggaggtggtt aaaataaaaa tatttgtaaa tgaattatta acagatgttg
     7861 aggaaaatac aactgcttat aaagttagag atagctataa caataaatgt gatgtattgg
     7921 tgttaaatgg atatccaatt aaaacagata tgaccctttg tgaaaatgat aaattaacac
     7981 ttatacaaaa aggagttaag ccatcacttg atgaattgga aagcttgttg attgctagac
     8041 atactccaaa tatacataat aaattaaaaa aagggaaagt tgctatattg ggattggggg
     8101 gacttggttc caatatagct atctctttag ctagaatagg agtgggagaa cttttactta
     8161 tagattatga tgttgtagaa ccatctaatt taaatagaca gcagtatttt atagatgata
     8221 taggaaaact aaaaactgat gccatgatag aaaatataaa aaaaataaat ccatttataa
     8281 aattaaataa acgagatatt tttttaaaca agaacaatat ggatactatt aaggattcag
     8341 atttaataat tgaggccttt gatgatgctt catgtaaagc acaggtatgt aattatgtat
     8401 taataaattt aaaagataaa tatttaatag catcatctgg tatggcaggt tactatgatt
     8461 caaatataat aactactaag aagataaaag acaggtttta tatatgtggg gattttgtga
     8521 atgaagctaa atttggtgaa gggcttatgg ctccaagggt tgccatctgc gcaaatcata
     8581 tggcaaattt ggcaacgcaa atacttatag atatgtaaat attaaaatta gatataaaaa
     8641 agattgaata ttaaaaaaat ttaacgtgga gggaaataat atggataaat tggtacttgg
     8701 tggacatgaa tttaatagca gattattggt tggaacaggt aaatatggtt caaataatat
     8761 attgcctgag gtaataaaag aaagtggaag tgaaataata acaatggcac ttagaagagt
     8821 ggatttagac aataaacaag aaaacatatt aacgtatatt ccaaaagaga tgacaatact
     8881 tccaaataca tcaggagcta ctaatgcaga agaagctgta agaatagcta gaatatctag
     8941 aaaaatggga tgtggagatt ttataaaaat agaagtaata tcagatacta gatatttact
     9001 tcctgataat gaagaaacta taaaagctac aaagatactt gctgatgagg ggtttatagt
     9061 tcttccatat atgacaccag acctttatgc tggaagaaga cttatagaag ctaatgcagc
     9121 ggcagttatg ccacttggag ctccaattgg ttcaaataga ggtcttcaaa tgaaagaaat
     9181 gataagaata atgatagatg agttggatat accaataata gtagatgcag gtattggaaa
     9241 accatctcaa gctatggagg ctatggaaat gggagctgat gcagtacttg tcaacactgc
     9301 tatagcttca gcaggagacc ctgtacaaat ggctagagca tttaaattag ctgtagaagg
     9361 tggaagagaa gcatatattg caaaaacagg aaatgtgtca gaatttgcaa atgcatcttc
     9421 tcctttaaca ggttttttag gcaatttata gaaagtggtg attggtgatg agtttttatg
     9481 atgtaataga aaaatatagg gattttgatt ttgatggata tttaaataat gttactgata
     9541 atgatgtttt aagaagttta tcaaaagaca agcttgaaga ttttgatata ttaaatttgc
     9601 tttctaaaac tgcagtaaaa catttagaag atatggcaca aaaagcacat aaattaagtg
     9661 ttcaatactt tggtaaaaca gtatgtttat atacaccaat gtatatagca aattattgtg
     9721 ttaatcaatg tgtttattgt agctataata taaagagtgg aataaaaaga aaaaaattaa
     9781 ctatggatga aattagagaa gagggagaag caatttcaaa agaaggtttc aaacatctac
     9841 ttgttttgac tggagaaagt agttttcact caagtgtaga atatataggt gaggctatag
     9901 aaatattaag agagaaattt ccctctatag gtatagaagt ttatccaatg gaagtagaag
     9961 aatataagta cattgtagat aaaggtgttg aaggattaac agtatatcaa gaaacttatg
    10021 acgaggaaat atataagaga gtacatataa aaggcccaaa atcaaattat aaatttagac
    10081 tagatgctcc tgaaagaggg gcaaaagcag gtatgaggac tttatcaata ggtgcacttt
    10141 tagggctaaa tgattttaga aaagaaactt tttttacaat attgcatggg aaatacttaa
    10201 aaacgaaata tcctcatatt gagctttcat attcaacacc aagaatgaga ccttttaaag
    10261 ggtgttttga ggaattggta gatataagtg atactgattt agttcaagct atggtatgta
    10321 tgagattatt tgaccctcat gcagctataa atatatccac tagagaaaat ttagaaatga
    10381 gaagtcatat aataccttta ggagtaacta aattgagtgc aggtgtatct actgatgtag
    10441 gaggtcattc tcaagatgaa catgatactg ctcaatttaa gataaatgat gaaagtactg
    10501 ttaaagatgt ggagaaaatg ctaaactcaa ttggttatca gcatgtgttt aaagattggg
    10561 aaagatttta aatgtatctg ataacaaaca gaaaattatg tagtgaggaa cgatatttag
    10621 aagttattaa agagtctata ttgagtggtg tagaaaatat aataataaga gaaaaggatt
    10681 tagaatatca agagctaaga aaattatata tgaaaataaa aactaaaatc aattgtatag
    10741 attttcaaga gcaaatatca gatgaaagtt tgaaaactaa tataaatcaa aaagagtgta
    10801 gaaataaatt caaggttaat tttataataa atagtaacat tgaatttttt
//
LOCUS       DACULQ010000128        10812 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.129, whole genome shotgun sequence.
ACCESSION   DACULQ010000128 DACULQ010000000
VERSION     DACULQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.129"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(233..3214)
                     /locus_tag="JA154_13600"
     CDS             complement(233..3214)
                     /locus_tag="JA154_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAT7996879.1"
                     /translation="MLPKEKHLEESIENHLVSIGYEQGHSQEFDLENCLFVDDLFRFL
                     ENTQKELLDEFKYNRGSNYKKAFIDLLNSNIKRRGLISVLRNGVEDIMMNGKFQLFYN
                     KPNLTTNKTAYENYKQNIFKITRQLKYSSKHNNTLDIVLSLNGFPVIVMELKNQFTNQ
                     NVYNAIKQFKKDRNPKETLFGFNQRVLVYFAVDTDEVYMTTELKGDKTFFLPFNKGNN
                     RGKGNPVVEGKEKTSYLWEEILTKDSLLDIIKRFYFIQEDDKGNQRAIFPRFHQLDAV
                     RKIVEDLKINKVGKNYLIQHSAGSGKTNTIAWTSHRLSSLHDEENNAIFDSIIVVTDR
                     KVLDKQLQNAIYQLEHKNGLVVKIDEDKNSSDLADAIVNRAKIIITTIQKFQYALPKI
                     EQLDKRRYAVIIDEAHSSTSGENMNALKETLAGKTLEEAKVIDAENEKNEKSSIDKMN
                     ELIEKRTNLGNISFFAFTATPKNKTMQVFGRIGEDGLPHEFHLYSMKQAIEEGFILDV
                     LKNFMPISVYYKVGKKIIDNPEFDKGEAKRAINKFVSLNEHNIRQKVETIVDDFVDNR
                     SMWLEGKAKGMIVTASRLHAVRYKLAIDQYIKEKGYDMKALVAFSGTVKDGDDEYTEV
                     GMNKEVAPDITDSNLPNIFDKNEFKLLIVAEKYQTGFDQPKLCAMYVDKKLDGVKTVQ
                     TLSRLNRTYPNKQTFILDFQNSVEDIQDAYKPYFEMTNIDKVTDPNVVNDLWYQLHEY
                     AVYTDEEVNDFTVLFYKEKRTSSQDAKMNNLIDKAVERYCYLEEDDEKKSDEFKKKCQ
                     KYITFYNFLIQIYPIKNLNLLRLQVYLVALLKKLPKKSKQRIDLDDMLSLDYYKLQKL
                     GLDDKKGTDISLYNGEGELVGISETATSRVSEADEEYLEDIIKKINDVFGVGLTEDDR
                     IMINTYEEMFKNDKDIMDIAKANSYEDLVSTFGKKYFKRGIVKTKNRNDRLVKEILTN
                     NNLQNYMIHYLAEKIYEEANR"
     gene            complement(3249..4520)
                     /locus_tag="JA154_13605"
     CDS             complement(3249..4520)
                     /locus_tag="JA154_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996880.1"
                     /translation="MDKNIFQININTDVLIRLRNQINEEYYISYRKEFYDKNNKKHRA
                     WDKICAIMDRLDDTVDYLNKLKLNTGKYRRSAFDFFEFMNQASVVMDCITHLAKIFEV
                     PYEEVETSTEIFNELGKEGKGTDKKYFEYLRSLCSVHPMETSRHKIYQENSFECSPYV
                     VWNDKSIWTNDASDLYAVVYTSKDNDSFKRIHIYMNQIFEYVKRRVNFIENIIEHIEK
                     YQQEIIEIFRNTPIKKEEDFESYIDYLRNLKKEIEIRYGNDSFHELNYLIELFDLPLS
                     NPDNEEKMNLYKNALKYAIKFEHNSIQNMTNEGYKNNGILYPQVNITTTLLEELISLN
                     SGSEERRKYRYAIEKTSYLSYDSCWSDREWAYDQIDDMMPFLERYVTFDKAQSDFEHY
                     VLVKLALYLECLENECLINKNVPNDKKFRIK"
     gene            complement(4582..5973)
                     /locus_tag="JA154_13610"
     CDS             complement(4582..5973)
                     /locus_tag="JA154_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAT7996881.1"
                     /translation="MGYRYRSDDELKDSGVEWLGKIPKDWEVRQLKRVLSDRKENNEP
                     IKTSNILSLTIEKGVIPYSEKGTGGNKAKEDLTQYKLVYPNDIVLNSMNVIVGAVGLS
                     KYFGCASPAYYMLYKINANDDIRFYNYIFKSKLFQDSLKGLGNGIMMKQSETSGKLNT
                     IRMRIPMEKLSSVKIPIAFNLDQEKIANFLDEKTSQFDSIISKKEKLIERLEEAKKSL
                     ISEVVTGKVKVVKTDDGYELIKRSSEEMKDSGVEWLGEIPKEWDIKRLRFLGKLQNGI
                     SKSSDSFGYGYPFVSYSNVYRNIELPHIVEGLVNSTEEERKIYSVEEGDVFFTRTSET
                     VEEIGFASTCMKTIDNSTFAGFLIRFRPNDKRLYKGFSKYYFRCDLNRKYFVKEMNLV
                     TRASLSQNLLNNLSVVLPKYEEQVEIYNALELKVSYINLSINKLKYQIEKLKEAKQSL
                     ISEAVTGKIEILD"
     gene            complement(5973..8000)
                     /locus_tag="JA154_13615"
     CDS             complement(5973..8000)
                     /locus_tag="JA154_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HAT7996882.1"
                     /translation="MSIDNNFNHVAFLWNIAESLRGTYKEEDYRKVMLPLIVIRRFDC
                     LLDDYDREIIKSVYEEYDFLPEEERDELVIVDLKENHDINLQFYNVSDFTWKKLLDDS
                     ENIKSNFEEYLNGFSNNVKEIIGKFKFKDEINQLDKKDKLYAVLSKMYEVDLHINSVS
                     NNKMGYIYEEMLRRFTENSAAGEQYTPREVIRLCMEILFMGKENFLTEEGKVISIADF
                     CCGTGGMLSIAEDYVEKINPSAIVNVYGQELLDESFAICQADMLMKGQNPDNIRLGNT
                     LTQDRFNGEKIRFLISNPPFGVTWKDEEKKVKEEADLGFDGRFGAGTPRVSDGSLLFL
                     QNMISKMYDDEEGSRIAIIFNGSPLFTGDAGSGESNIRKWIIENDLLEGIIALPTDMF
                     YNTGIATYIWVLTNKKEAKRKGKIQLVNASEYYQLMRKSLGNKRKEISLEQIEQIKEI
                     YEKFEESENSKIFDNEDFGYRKVTIERPLKLSFKVNEEAIENVKNTTQFINLAVSKKK
                     DIEVKEKEEAEGKIKQDKLIKLLESFDNTLEYMDRDKFIKDLKAKSKEFDIQLSAGLI
                     KAIVNTIGVRNEDAEVCKDSKGNIESDSSLKDTESIGLKDDVYEYFEKEVKPHVNDAY
                     IDESSINNIGYEIPFTRHFYKYEELRSFAEIMKEVESLESEIALKIRKVLG"
     gene            complement(8115..8464)
                     /gene="ssrA"
                     /locus_tag="JA154_13620"
     tmRNA           complement(8115..8464)
                     /gene="ssrA"
                     /locus_tag="JA154_13620"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8622..9113)
                     /locus_tag="JA154_13625"
     CDS             complement(8622..9113)
                     /locus_tag="JA154_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HAT7996883.1"
                     /translation="MEQISSKEGEIYMAGKKILATNKRARHEYFIEETYECGIELKGT
                     EVKSIRQGKVNLSDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIR
                     KLIGATTIKGYSLIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMT
                     GKY"
     gene            9732..10559
                     /locus_tag="JA154_13630"
     CDS             9732..10559
                     /locus_tag="JA154_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HAT7996884.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGSGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT         3340 a         1860 c         1157 g         4455 t
ORIGIN      
        1 aatatgaata acgataaacc atttatagag tttgaagaag aagttgattt tatacttact
       61 gagtgtaaaa aagaaggtgt ttctatagat agagatacta tagagcttat tcttgattta
      121 gacttaaagt ttttagaatt aaaaggaatt gctacacctt tagaagatga ataatataca
      181 atagctcata ataaagtacc tatggagagt taattagctc tctataggta tcttatctat
      241 ttgcctcttc atatatcttt tcagctaaat aatgaatcat ataattttgt aaattattat
      301 tagttaatat ctccttaact aatctatcat ttctattttt ggttttaact atacctctct
      361 taaaatactt cttaccaaaa gtgctaacta aatcttcata actattagct ttagctatat
      421 ccatgatatc cttgtcattc ttaaacatct cttcataagt atttatcata attctatcat
      481 cttccgtaag tcctacacca aaaacatcat ttattttttt aattatatcc tctaaatact
      541 cttcatcagc ttcacttaca cgagaagtcg cagtttcaga tataccaact aactcacctt
      601 ctccattata taaggatata tccgttcctt ttttatcatc taatccaagc ttttgcaatt
      661 tataataatc aaggcttaac atatcatcaa ggtctattct ttgtttactc tttttaggta
      721 acttctttaa aagtgctact aaataaactt gcaatcttaa aagatttaag ttctttatag
      781 gataaatctg aattaaaaaa ttataaaaag taatatactt ttgacatttt ttcttaaact
      841 catcagattt tttttcatca tcttcttcta aataacaata cctttcaact gccttatcaa
      901 ttaaattatt catcttagca tcttgagaag aagttctttt ctctttatag aataatactg
      961 taaaatcatt tacttcctca tcagtatata ctgcatattc atgtaactga taccaaaggt
     1021 catttactac atttggatct gttaccttat caatattagt catttcaaaa tatggcttat
     1081 atgcatcttg tatgtcttcc acactatttt ggaaatctaa tataaatgtt tgtttattag
     1141 gataagttct attaagcctt gataaagtct gaacagtttt aacaccatct agttttttat
     1201 ccacatacat agcacaaagt tttggttggt caaatccagt ttgatacttt tctgcaacta
     1261 ttaatagctt aaattcattt ttatcaaata tattaggtaa atttgaatca gtaatatctg
     1321 gtgctacttc cttattcata cctacctctg tatactcatc atcaccatct tttacagtac
     1381 cagaaaaagc aacaagtgct ttcatatcat atcctttttc ttttatatat tggtctatag
     1441 ctagtttata tctaacagca tgtaatctac ttgcagtaac tatcatacct ttagctttac
     1501 cttctaacca catagaccta ttatctacaa aatcatctac tatagtttca accttttgcc
     1561 ttatattgtg ctcatttaaa ctaacaaact tatttatagc cctcttagcc tctcccttat
     1621 caaactcagg gttatcaata attttctttc ctactttgta ataaacactt ataggcataa
     1681 agttttttag tacatctaat ataaatcctt cctctatcgc ttgtttcata gaatataaat
     1741 gaaattcatg aggtagtcca tcttcaccta tcctcccaaa tacttgcata gtcttattct
     1801 taggagtagc tgtaaaagca aagaaactta tatttcctaa atttgttctt ttctctataa
     1861 gctcattcat cttatctata ctagactttt cattcttttc attttccgca tcaattacct
     1921 tagcttcttc taaagtcttt cctgctaaag tttcttttaa tgcattcata ttttcaccag
     1981 atgttgaaga atgtgcttca tctataatta ctgcataacg ccttttatca agttgttcta
     2041 tcttaggtag tgcatactgg aatttttgaa tagtagttat aataatcttt gctctattta
     2101 caatagcatc tgctaagtct gatgaatttt tatcttcatc tatcttaaca acaagtccgt
     2161 ttttgtgctc taattgatat atagcatttt gtaattgttt atcaagtacc tttctgtctg
     2221 taactacaat tatagagtca aatatagcat tattttcttc atcatgaaga cttgataacc
     2281 tatgtgaagt ccatgctata gtgttagtct taccactacc agcactatgt tggataagat
     2341 aatttttacc tactttattt atctttaaat cttctactat cttccttact gcatctaatt
     2401 gatgaaatct tggaaatatt gctctttgat tacctttatc atcttcttga ataaagtaaa
     2461 atctttttat aatatcaagt aaactatctt tagtaagtat ttcttcccaa agataagaag
     2521 ttttttcttt tccttctact acaggatttc ccttacctct attgttacct ttattaaacg
     2581 gtaagaagaa tgttttatct ccttttagct ctgttgtcat ataaacttca tcagtatcta
     2641 ctgcaaaata tactaacact ctttgattaa atccaaataa agtttctttt gggtttctat
     2701 cttttttgaa ttgctttata gcattataca cattttgatt agtaaactga tttttaagtt
     2761 ccattactat aacgggaaat ccatttaaag ataatactat atctaaagta ttgttgtgct
     2821 ttgagctata ttttaattgt cttgttattt tgaatatatt ttgtttataa ttttcatacg
     2881 ctgttttatt agtagttaga tttggtttgt tgtagaataa ttgaaatttt ccgttcatca
     2941 ttatatcttc tacaccattt ctaagtacac ttataagacc tcttcttttg atgtttgaat
     3001 ttaataaatc tataaaagct tttttgtaat tacttcccct gttgtattta aactcatcta
     3061 gtaattcttt ttgagtattt tctaagaatc taaataaatc atctacaaat aagcaatttt
     3121 caaggtcaaa ttcttgtgaa tgaccttgtt catatccaat tgaaactaaa tgattttcaa
     3181 tagattcttc taggtgtttt tcttttggta acataatatc ctccctctcc ctattttatt
     3241 agctataatt atttaatacg aaacttttta tcattaggaa cgttcttatt tattaaacat
     3301 tcattttcta agcactctaa ataaagtgct agttttacca atacataatg ctcaaaatca
     3361 ctttgagctt tatcaaatgt tacgtatctt tctaaaaaag gcatcatatc atctatttga
     3421 tcatatgccc attctctatc actccaacaa ctatcatagc ttagatatga tgttttttct
     3481 attgcatatc tgtacttacg tctctcctca ctaccactat ttaatgaaat tagctcttct
     3541 agtaatgtag ttgttatatt cacttgtgga tataatattc cattattctt ataaccttca
     3601 tttgtcatat tttgaatact attatgctca aatttgatag catattttaa agcattttta
     3661 tataagttca tcttctcttc attatcagga tttgataatg gcaaatcaaa taattctata
     3721 aggtagttta attcatgaaa agaatcatta ccatatctta tttctatctc tttttttaga
     3781 tttcttaaat aatctatata actttcaaaa tcttcttctt tctttattgg agtattccta
     3841 aaaatttcaa ttatttcttg ttgatacttt tctatatgtt ctattatatt ttcaataaaa
     3901 tttactcttc tttttacata ctcaaatatt tgattcatat atatatgaat tcttttgaaa
     3961 ctatcattat ctttacttgt gtataccact gcatataaat cgctagcatc attagtccat
     4021 atacttttat cattccaaac tacataagga ctacattcaa aactattttc ttggtatatt
     4081 ttgtgtctac tagtttccat tggatggact gaacacaatg aacgtagata ttcaaagtat
     4141 tttttatctg tacctttacc ctcttttcca agctcattaa aaatttcagt tgaagtttca
     4201 acttcttcat atggtacttc aaatattttt gcaagatgtg ttatacaatc cattactacg
     4261 gaagcctgat tcataaattc aaagaagtca aatgcacttc gtctatattt acctgtattt
     4321 aatttgagtt tatttaaata atctacagta tcgtcaagcc tatccattat tgcacatatt
     4381 ttatcccaag cgcgatgttt tttattattt ttatcataaa attcttttct ataagatata
     4441 taatattctt catttatttg attccttaat cttatcagta catctgtatt tatattaatt
     4501 tgaaatatat ttttatccat taattctccc ttttatttaa ctttgtatgt ataatctata
     4561 tctatattaa gatactagaa gttaatctag tatctctatt tttcctgtta ctgcttcact
     4621 tattaaactt tgtttagctt cttttagttt ttctatttgg tactttaact tattaatcga
     4681 taaattaatg tagctaactt ttaattcaag tgcattatat atctcaactt gttcttcata
     4741 cttaggcaat acaacagaaa gattatttaa aagattttga gataaagatg ctctagttac
     4801 taaattcatc tcttttacaa aatattttct atttaaatca catctaaagt aatatttaga
     4861 aaatcctttg tatagtcttt tatcattcgg tctaaacctt attaaaaatc ctgcaaaagt
     4921 agaattatca atagttttca tacacgtaga agcaaaacca atttcttcta ctgtttctga
     4981 agttcttgta aagaatacat ctccttcttc tactgaataa attttacgtt cttcttcagt
     5041 tgaatttact agaccttcta ctatatgtgg taattctata tttctatata cattactata
     5101 actaacaaat ggataaccat aaccgaatga atcactactt ttacttatac cgttttgtag
     5161 ttttcctaaa aatctaagtc tttttatatc ccattcctta ggtatctcac ctaaccactc
     5221 aactccacta tccttcatct cttcactact tctctttata agctcatacc catcatcagt
     5281 cttaacaacc ttaaccttac cagtaacaac ctcacttata aggcttttct ttgcttcttc
     5341 taatctttct attaactttt ctttcttaga tataattgaa tcaaactgtg aagtcttttc
     5401 atcaagaaag tttgctattt tttcttgatc taagttaaaa gcaattggta tctttactga
     5461 acttagtttt tccattggaa ttctcattct gatcgtattt aacttacctg aagtttctga
     5521 ttgtttcatc attattccat tacctaatcc ttttaaacta tcctggaata atttagattt
     5581 aaaaatataa ttataaaatc ttatatcatc gttagcattt attttatata acatataata
     5641 tgcaggactc gcacacccaa aatattttga tagtcctact gcaccaacaa ttacattcat
     5701 actatttaat acaatatcat ttggatatac taatttgtat tgtgttaaat cttctttagc
     5761 tttattacca ccagtacctt tttcagaata tggaataact cccttttcta ttgtcaatga
     5821 cagtatatta cttgttttta taggttcatt attttctttt ctatctgata gtaccctttt
     5881 taattgtctg acttcccaat ccttaggaat cttccctaac cactcaactc cactatcctt
     5941 taactcatca tcacttctat atctgtatcc cattacccta gcaccttcct tatctttagt
     6001 gctatctcac tttctaaact ttctacttcc ttcataatct cagcaaaact tcttaactct
     6061 tcatacttat aaaaatgtct agtaaatggt atctcatatc ctatattatt tatactactt
     6121 tcatctatat aagcatcatt aacatgaggc tttacttcct tttcaaaata ctcatataca
     6181 tcatctttaa gacctatact ttcagtatct tttaatgaac tatcactttc tatattacct
     6241 ttgctatctt tacaaacttc tgcatcttca tttcttacac caatagtatt aactatagcc
     6301 tttataagtc ctgctgaaag ttgtatatca aactcttttg atttagcttt taaatctttt
     6361 ataaatttat ccctgtccat atactctaaa gtattgtcaa agctttctaa aagttttatt
     6421 aacttatctt gttttatttt tccttcagct tcttcttttt ctttaacttc tatatctttt
     6481 ttcttagaaa ctgcaagatt tataaattga gtagtatttt ttacattttc tatagcttct
     6541 tcgtttactt taaagcttag ttttaaaggt ctttctattg ttacttttct atatccaaaa
     6601 tcttcgttat caaatatttt actattttca ctttcttcaa atttttcata tatttctttt
     6661 atttgttcta tttgttcaag acttatttct tttcttttgt ttcctaagct ttttctcatt
     6721 aattgataat attcactagc atttacaagt tgtatcttac cttttctttt agcttctttt
     6781 ttgttagtaa gtacccaaat atatgtagct attcctgtat tgtagaacat atcagtaggt
     6841 agtgctatta taccttctag taaatcattt tctattatcc attttctaat gtttgattct
     6901 ccacttcctg catctcctgt aaataatgga cttccgttaa atattatagc tattctactt
     6961 ccttcttcat catcatacat tttacttatc atattttgta ggaaaagtaa tgaaccatca
     7021 cttactcttg gagtacctgc tccaaatctt ccatcaaaac ctaagtctgc ttcttctttt
     7081 actttctttt cttcgtcttt ccaagttaca ccaaaaggcg gatttgatat taaaaatctt
     7141 attttttctc cgttaaatct atcttgtgtt aaagtatttc caagtcttat gttatctgga
     7201 ttttgccctt tcattagcat atctgcctga catattgcga atgattcatc aagtagttct
     7261 tgtccatata catttactat tgcacttgga tttatttttt ctacatagtc ttcagctatt
     7321 gaaagcattc ctccagtacc acaacaaaag tctgctatag atattacttt tccttcttct
     7381 gtaaggaagt tttctttacc cataaatagt atttccatac aaagtcttat aacttctctt
     7441 ggtgtgtatt gctctcctgc tgctgagttt tctgtaaatc ttcttaacat ttcttcgtat
     7501 atgtatccca ttttattgtt agatactgag tttatatgta gatctacttc atacattttg
     7561 ctaagaactg catatagctt gtctttttta tctaattgat ttatttcatc tttaaattta
     7621 aatttaccta ttatttcttt tacattgttt gagaatccgt ttagatattc ttcaaagttt
     7681 gattttatat tttcactatc atctaatagt tttttccatg taaaatcact tacattgtaa
     7741 aattgtaagt ttatgtcgtg attttctttt aagtctacta ttactagctc atctctttct
     7801 tcttctggta agaagtcgta ttcttcgtat actgatttta ttatttctct atcataatca
     7861 tctaatagac aatcaaatct tcttattact attagtggta acattacttt tctatagtct
     7921 tcttctttat atgtacctct taaactttct gctatattcc acagaaatgc aacatggtta
     7981 aaattattat ctatactcac ttatactatc tccttttagt taaattttac attagtttat
     8041 attataatta tatcatttca aggcacaaaa aaaacatatt gactaaacta tcaatatgtt
     8101 ttcgtggatt tcaatggtgg agacgagggg agtcgaaccc ctgtccgcaa atcttttcct
     8161 tgctgcatct acatgcttag ccaacttcac taatatctca cctcacatag cgccaagtgg
     8221 caggctctat gcttagccag tcccaattaa tctacttcaa gtcgagacac ccctaaagta
     8281 gttccctgca taatgacgtc cagtcttagt cggcaggagg actaagaggg acgagcagca
     8341 actaggctgc tatcgcgaaa ttatcgtctg cgtttatttt aaagttcccc agtttttacg
     8401 tgacccagag taacacgaca tgcagcctca agttccaaac ccacgtcgaa acctttacgc
     8461 ccccttgagt agtttatagt ccagtagact tttctcaaac tttatcacca tttttattaa
     8521 atttttaatg tatatataat ataatacttt taagaataaa aatcaatgtt atgttctaga
     8581 taataaagaa ttttattcta aaaaatacag ttttatctag tttaatactt acctgtcatt
     8641 tctctttcta tacgtctttg tgcatctttc ttagccatat cttgacgttt atcatatagt
     8701 tttttacctt ttgctaaggc aatctctact ttaactttac cgttttttag atacaaacta
     8761 agtggtataa gtgaataccc ctttatagta gttgctccaa taagctttct tatttcatgc
     8821 ttatgaagta atagttttct aactcttaaa gaatcaacat tgaatatatt tccttgctca
     8881 tatggactta tatgaacttg ttttaaaaat acttcgctat tatctacaga tgcaaatcca
     8941 tcacttaggt taactttgcc ttgtcttatt gattttactt ctgttccttt taattcaatt
     9001 ccacattcat aagtttcttc tataaaatat tcatgtcttg ctcttttgtt tgttgctaat
     9061 atttttttac cagccatata aatctcacct tctttgctag aaatttgttc cataaatgtt
     9121 agaaatttac taaatattta atgttgccaa attatttgac taacttgtat gataacatat
     9181 ttaggcattt tagtcaatat ttcactgata tattaagtta gttaggctat atataattaa
     9241 gtaatataaa tgtatgaaaa tagctaaaaa actacttcga atatacttaa tactaaacta
     9301 actctaacca gtaatactaa tttataaatt tcaaaacaaa aatattacat attataaatc
     9361 ttctatcttc tatcttctat cttctatctt ctatcttcta tcttctatct tctatcttct
     9421 atcttctatc ttctatcttc tatcttctat cttctatctt ctatcttcta tcttctatct
     9481 tctatcttct aaataatttt aacatgccca taaaatataa tacccacaaa tgataaataa
     9541 tactcaaaat gtctattgaa ataactatac atccttaaat aacttaactt aaatctaaac
     9601 atttcaaaaa cttcttaaaa atatgtaaaa atttgatgat aaagtagcga ttttttcaaa
     9661 aaaaatgtaa tatagagtta tatcttataa tattttagag ataaatttta tcatagtggg
     9721 gagaaatttg tatggattta agcaaaaaag taaaaatttt agaaaatgca tcagatggca
     9781 aaagcgaatt tgagatatta gaatttgaaa aacttgaggg agcttctagc ccattaatgg
     9841 caatgggatt atattttgct agagaagctg gtttgaaagc tagacaaatt aagataaaat
     9901 taaataattc atcaataaaa acagaagctg gtgcattata ctattataaa ggaaatatat
     9961 cttctgaaac taagattggt ggtgttggag gtctttttaa aaaaggaata tcaggagcac
    10021 ttactaatga atcaatgatg aaacctgtat acaaaggtac tggagaagtt tatttggaac
    10081 cttcttttag acattattta tttatgagtc ttgataatga ttctataatt gttgataaag
    10141 gattattcta ttgttgctct gatacaatag aagtaaaagc atttacacaa aagaacttct
    10201 catctgcttt acttggtggt gaaggcttct tccaaataga attaagtggt actggtgtaa
    10261 tcgttctgga atgtgttgta cctcaaagtg aaattgtaga gtatgaattg aaaaatggtg
    10321 aagaattaaa ggtagatggt aactttgcaa tagcaagaac atctggtgtt aatttttctg
    10381 ttacaaaatc agataaatct ttaattggtt ctgctttaaa tggtgaaggt ttcttaaata
    10441 catttactgg tagtggtaca gtttggctag ctccaactca agtaatgtat gaaaaactaa
    10501 gttttggagc aatgcctact aatgataata tgaataataa agtttctaag caaaaatagt
    10561 tgttttataa atatttatta aatcattatt gattttataa agtcactata aattttatta
    10621 gattattata aaatcagagt gtggaaatat ttatgtgtat tctttaaata aaaatttcca
    10681 cattctgtaa agttattata cttgatatat aaaattcaat ttaggaacat agtctaacat
    10741 ttataaacat attaaggtaa tgatttctct tggtagttat tctcatatct tattctttga
    10801 ttatatttat ct
//
LOCUS       DACULQ010000129        10753 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.130, whole genome shotgun sequence.
ACCESSION   DACULQ010000129 DACULQ010000000
VERSION     DACULQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.130"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            5..1324
                     /locus_tag="JA154_13635"
     CDS             5..1324
                     /locus_tag="JA154_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAT7996885.1"
                     /translation="MDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILLA
                     PSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYPN
                     LNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHHI
                     VSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERTQ
                     EEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIAI
                     LRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEAL
                     KDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTDL
                     SLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            1566..2672
                     /locus_tag="JA154_13640"
     CDS             1566..2672
                     /locus_tag="JA154_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAT7996886.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            2809..3015
                     /locus_tag="JA154_13645"
     CDS             2809..3015
                     /locus_tag="JA154_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAT7996887.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            3032..4147
                     /locus_tag="JA154_13650"
     CDS             3032..4147
                     /locus_tag="JA154_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HAT7996888.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            4148..6049
                     /locus_tag="JA154_13655"
     CDS             4148..6049
                     /locus_tag="JA154_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086468.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAT7996889.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKRYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            6069..8495
                     /locus_tag="JA154_13660"
     CDS             6069..8495
                     /locus_tag="JA154_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAT7996890.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            8564..8860
                     /locus_tag="JA154_13665"
     CDS             8564..8860
                     /locus_tag="JA154_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAT7996891.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            8860..9120
                     /locus_tag="JA154_13670"
     CDS             8860..9120
                     /locus_tag="JA154_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAT7996892.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            9138..9449
                     /locus_tag="JA154_13675"
     CDS             9138..9449
                     /locus_tag="JA154_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HAT7996893.1"
                     /translation="MNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNMLD
                     VKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFKVE
                     G"
     gene            9453..9860
                     /locus_tag="JA154_13680"
     CDS             9453..9860
                     /locus_tag="JA154_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT7996894.1"
                     /translation="MACETIKMEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVA
                     VSEACTNAIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLG
                     LFIIKTLMDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            9860..10633
                     /locus_tag="JA154_13685"
     CDS             9860..10633
                     /locus_tag="JA154_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B/F/G family RNA polymerase sigma-70 factor"
                     /protein_id="HAT7996895.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
BASE COUNT         4403 a         1113 c         1985 g         3252 t
ORIGIN      
        1 tattatggat atagtttctt tatgggacaa aaccctacaa ttaataaaag gtgacttaac
       61 ttcagtaagt tttaatacct tttttaaaaa catcgtacca ttaaaaatac atcttaatga
      121 tttaatctta ctggctccta gtgattttaa taaagatatc ttagagaata gatatttaca
      181 tttaattgaa gatgctatta gtcaactgtc attaaaaaaa tataatatta aatttgtatt
      241 gagcgaaaaa gaggttgctg atttaaattc tgatagcaca gatttaaatt atagagttct
      301 ttaccccaat ctaaatccaa aatacacatt tgatacattc gttattggta atagtaatag
      361 atttgcacat gctgcatgcg ttgcagttgc agaatctcca gctaaagctt ataacccact
      421 ttttttgtat ggaggagttg gattaggtaa gactcaccta atgcatgcta taggtcatca
      481 tattgttagt caaaaaaaag actcaaaagt agtttacgtt tcttcagaaa aatttactaa
      541 tgaacttata aactctataa aagacgataa aaacgaagaa tttaggaata aatatagaaa
      601 cgtagatgta ttactaatcg acgatattca atttatagca ggaaaagaaa gaactcaaga
      661 agagtttttc catacattca atactcttca tgaagcaaac aaacaaataa ttatatctag
      721 tgatagaccg ccaaaagaca tacctacact agaagatagg ctaagatcga gatttgaaat
      781 gggacttata actgatattc aagcacctga ctttgagaca agaattgcta tacttagaaa
      841 aaaggctcaa ctagaacgta tagatgtgcc aaatgaggtt atgtcatata tagcaaaaaa
      901 tattaagtca aacataaggg aactagaagg tgcattaact agagtggttg cttattcttc
      961 tcttagcaat agggtaatat cttttgatct tgctacagaa gctttaaaag atattataac
     1021 aacttctaaa aatgaagaaa taaatgttct cagaataaaa gaaaaagtat cttctgtatt
     1081 caatttaaag atggaagatt ttaattctaa aaaaagaaca cgttcaatag cttatccaag
     1141 acagatagct atgtatttaa cgcgcgagct tactgattta tcccttccta aaataggtga
     1201 agagtttggg ggtagagacc atacaacagt aattcatgct catgacaaag tatctaagga
     1261 tatagaagaa agtgaagaaa ttaagactaa aatagacaaa attatatcag atttgaaggg
     1321 ataatttata cacaattgta tgtttatatc tcttgaataa cctgttaata ctttttttga
     1381 acaaaaatta ttaacaaatt atcaagtcta aaataacaaa gatattaaca tcttatacac
     1441 atgttaatat ctttgtattt ataatgttat acatacttat taacattatc aacaacacct
     1501 actactatta ctactatgat tttaacttat ttttatatat aaaaagccag aggaggttat
     1561 ccacattgaa aataatttgt aatcaaaaaa tcctagcaaa tagaattgga attgctcaaa
     1621 aagctattaa tggaaaaaca acaatagaat tattaaaagg tatacttata tctacagaag
     1681 aaggtcaatt aaaacttact ggatatgatg ctgaaatagg aatagaaact tatgtacaag
     1741 cagaaatcat agaaaaaggt gatgttgttg tagatgcaag gctatttgga gatataatta
     1801 gaaaattacc agattccttt gttgagatag aaactgattc agaaaataat atttacataa
     1861 attgtgtaaa ttcaagattt aaaattaaag gttatgcagc aaaagaattt cctaaattac
     1921 cagaattaaa tgaagaagat ttatatagca tacctcaaga aatcctaaaa aatatgatta
     1981 aacaaactgt atttgctatc tcacaggacc aaacaaaacc tgttttaatg ggagaacttt
     2041 tagaaattgt agatagaaac ttaaatttag tagcaattga tggatataga ttagctgtaa
     2101 aaagttgttc ggttgatagt ttaactgaaa atataaaagt cataattcct ggaaagacac
     2161 ttatagatgt aaatagtttg ctttctggag aggataatgt taaagttgga tttaacgaaa
     2221 aaaatgctat ttttataatt aatgatacga aaattattac aagattgctt gaaggagatt
     2281 ttattgatta taaaaaatta ttgccaagag aacataacag cagagttaaa ttaaatacta
     2341 aggaactttt aaatagtata gagagagcgt ctttattgtc tcaatcagaa aaaaataatc
     2401 ttataaagtt atctataaga gataaagtaa tggcaattac ttctaataca gaaaaaggaa
     2461 atgtgtatga agaagttgaa atagatttag atggagatta cctagatata gctttcaatt
     2521 ctagatattt tatcgaaggt ttgaaaaata tagataatga ggaaatattt atagaattta
     2581 ctacaaatgt aaatccttgt ataattaaac caacagatga tgttaattat atttacttac
     2641 tgcttccagt aagaatatca tcaaatatat agcagacatg aaggagttgt cttttgtaga
     2701 aaatttttct aatcttagac aactcctttt ataagcatat ttatggtata atatactttt
     2761 agtgtatcaa taggaatttg tttaaaatag tgacaaggag gaactattat gactgaaata
     2821 actatagaat cagaatatat aaagttggat caatttctta aattagcaga gattgcatca
     2881 acaggaggtc atgcaaaatt tttaattcaa gaaggtttag tgacagtaaa tgatgaaata
     2941 gaattaagaa gaggaaaaaa gataaaatca ggagatatag ttgaaataga aggaactaaa
     3001 ataaaagtat tgtaaaaaag gagaactttt tgtgaaactt aaaagtttgc aacttgttaa
     3061 ttttagaaat tataaaaagt tgcatctgga atttaatggg aaagtaaatc tacttgttgg
     3121 taaaaatggt caaggaaaga caaatatagt agaatctatt tatatgcttt cttttggaaa
     3181 atcatttagg actaacaaag ataaagaaat ggttagattt aatagtgaaa acttatatat
     3241 tggaggaagt ttttcaaaat ataataaata tagtctaata gagctaatta ttggaaaaga
     3301 taaaaaaggg ataagaataa ataaagttcc tttacaaaag atacaagaac tacttggcaa
     3361 cttaaatgtt gttatatttt ctcctgagga tttaaggttg gttaaagagg gtcctaaaga
     3421 aagaagggca tttatagaca aagagattag tcagattata cctaaatatt ataaatatct
     3481 taccaattat aataaaacac tttctcaaag gagtagggtg cttaaaaata tacatgttga
     3541 tgaagcttta ttagatgtat atgatgatac gttagcaaag tatggaagtt atatttatat
     3601 tttgcgtaga gactttataa aaaagatagc taatatatct gaaaatatgc atatgaattt
     3661 gactaatgga gttgaaagat tatcaattcg atataaaaat caaataaata taactgatga
     3721 agatactata gatacagtat ataataaatt tttagccaag ttgtcatcga atagacctaa
     3781 tgatatagaa tctaagacta ccagatatgg tatacacaaa gatgacttaa atatattcat
     3841 aaatgattta gatgcaagac tttttggttc acaaggtcaa caaagaactg cttcaatatc
     3901 attaaagtta tcagagatag aattaataaa aaatgaagtt gaagaatatc cagtattaat
     3961 tttagatgat gtttttagtg agttagatga agcaagacaa aaacttttag taaataattt
     4021 aagcaatgtt caaatgttta taacaagtgc tgaagtttca cataaaaaaa tatttgatga
     4081 aaaaaatgtt acaatattta atattgaaaa tggcgatgtt attagcatag agaatggagg
     4141 aaattaaatg aaacaagaat acggtgcaag tcagatacaa gtattagagg gattagaagc
     4201 cgttagaaaa agaccaggta tgtatattgg tagtacaagc cctagaggtt tacatcactt
     4261 agtttatgaa gttgtagata atagtataga tgaggcttta caaggatatt gttcagacat
     4321 atatgtatct attaatgaag atggcagtgt tttagtaaaa gataacggaa gaggtatacc
     4381 tgttgaaata catcctaaaa ctggtaaatc aactttagaa acagtattaa ctaatcttca
     4441 tgcaggagga aagtttggag gcggaggata taaagtatct ggaggtcttc atggagttgg
     4501 tgtttctgta gttaatgcat tgtcaaaatg gatggtagca gaggtttatt taaacggaaa
     4561 gagatataag cagacatatg agaaaggatt gccaacatct aagcttgaag tagttggaga
     4621 atctcaagat aaaggaacaa tgattcaatt tatgccagat gagacaatat ttgatgaaat
     4681 agagtttaaa tatgaaacat tggagtatag gcttagagaa ctttctttct tgaataaagg
     4741 aataaaaata gtatttgaag ataaaagaga aggacaagaa aaaagaaaag aatttcacta
     4801 tacaggtggt ttggtagaat atattaagta tttaaataaa tctagaactg gtatacatga
     4861 tgacatagtt tatatagata aaaaggttga tgattgtttt gttgaactag ctatgcaata
     4921 tacagatggt tatacagaga acatatattc ttttgcaaac aatataaata cacatgaagg
     4981 tggttctcat ttaagtggat ttaaagcagc tcttactaaa actgtaaatg attatgctaa
     5041 gagaaataaa tttttaaagg aaaatgatgt taacctactt ggtgaagaca taagagaagg
     5101 tcttacagca gtagtatcag ttaaattacc agaacctcaa tttgaaggtc aaactaaaac
     5161 aaagctaggt aatagcttta tgagaggtat agttgatagt gtaacagttg atgaactggg
     5221 gtctttccta gaagaaaacc catcaacagc aagaatcata gttgataaag ctttaagagc
     5281 acaaagagct agagaagctg caaaaaaagc aagagaatta acaaggagaa aaagtgtatt
     5341 agaaagtaca tctttacctg gaaaacttgc agattgtgca gaaaaagatc catctaaaag
     5401 tgaaatattc ttagtcgaag gggattcagc gggaggttca gctaaacaag gtagagatag
     5461 aaatagtcaa gctatacttc cattaagagg gaaaatactt aatgttgaga aatcaagact
     5521 agataggata ctatcttcag atgaaataaa aaatatgata acagcttatg gttgtggtat
     5581 tggagaagat tttgatatag ataaggctag gtatcataaa attataatta tgaccgatgc
     5641 tgatgtagac ggagcccata taagaacttt attattaaca ttcttcttta gatatatgag
     5701 acctcttata gatgaggggt atgtttatgc agcacagcca cctttatata aagtgacaaa
     5761 gcaaaagaag gaacattatg tttactcaga taaagagtta aatatcttat tagatgaaat
     5821 tggaagaaat ggtgttgaac ttcaaaggta caaaggtctt ggagagatga atgcagagca
     5881 attatgggaa acaactatga atcctgagac aagaacttta ttacaagtta ctgtagaaga
     5941 tgctgcaatt gcagatgaag tattttcaat gcttatgggt gataaggttg ctccaagaaa
     6001 agaatttata gaagaaaatg caagatttgt tagaaactta gatatataga gagaggggat
     6061 tagtatacat ggaagaaaat aacaaaatac tccctattga aatagcggaa gaaatgaaaa
     6121 aatcgtatat tgattattca atgagtgtta tagctggacg tgctcttcct gatgttagag
     6181 atggtttaaa gccagttcat agaagaatat tatattcaat gagtgagtta aatttaactc
     6241 cagataaacc atacaggaag tcagctcgta ttgttgggga cgttttaggt aagtaccatc
     6301 ctcatggaga tactgctgtt tattatgcta tggtaagaat ggcacaagat ttttcaacta
     6361 gagcactttt agtagatggt catggtaact ttggttctgt tgatggggat tcaccagctg
     6421 ctatgcgtta tacagaagct aaaatgagta aattatcatt agaactactg agagatattg
     6481 aaaaggaaac tgtagacttt aaaccaaact ttgatgagtc gttaaaagag ccttcagtat
     6541 tgccagctag atatcctaat ttattagtaa atggctcaaa tggtatagct gttggtatgg
     6601 caacttcaat acctccacat aatttagcag aagtaattga tgcaactgta tatttgatag
     6661 ataatccaga gtgtagtgta gatgatttaa taaaatttgt tcaaggacca gatttcccta
     6721 cagctgcaat tataatggga aaagaaagta tagcagaagc atacagaact ggaagaggaa
     6781 aagttaaagt taggtctaga gcttttatag aagagctacc aaaaggaaaa cagcaaataa
     6841 tagttacaga aataccttat caagtaaata aggctaaact ggttgaaaga atagcagagt
     6901 tagttaaaga aaagagaata gaaggtatat cagacctaag agacgaaagt aatagaaatg
     6961 gtatgagaat tgttatagaa ttaaagaggg atgctaatgc taatatagta ttaaataatt
     7021 tgtataaaca ttctcaaatg gaagatactt ttagtataat aatgcttgca cttgtagatg
     7081 gtcagccaag agttttaaat cttaaacaaa tattatatca ttatattaaa catcaagaag
     7141 atgttgttac aagaagaact aaatttgaac taaataaagc tgaagcaaga gcacatattt
     7201 tagaaggatt aaagattgct ttagataata tagatgctgt tataagcttg ataagagctt
     7261 caaagactgg gcaagaagct aagctaggtt taatagaaaa attcaaatta actgaaatcc
     7321 aagcacaagc tatattagat atgagacttc aaagacttac aggtttagaa agagataaga
     7381 tagaagctga atatgaagat ttaatcaaga agataaatag attaaaagag attttagctg
     7441 atgaaagatt acttttaaat gtaataaagg atgaaattac aataataaaa gaaaattact
     7501 ctgatgagag aagaacagaa ataagacatg ctgaaggcga aatagatatg agagatctta
     7561 taagtgatga agaaatagca ataactctta ctcactttgg atatataaaa aggcttccat
     7621 ctgatactta taagagtcaa aaaaggggtg gaagaggtat ttcagcactt acaactagag
     7681 aagaagattt tgtaaggcac ttggtaacta caactactca tagtagacta ttattcttta
     7741 caaataaagg tagagtattt aaattaaatg catatgaaat accagaaggt aagagacagg
     7801 ctaaaggtac tgctatagtg aatttacttc aattgtcagc agatgagaaa attgctactc
     7861 taatacctat tgatggtaat gatgaaaatg aatatttatt acttgcaacg aaaaaaggta
     7921 ttgttaaaaa gactaagaga gaagagttca aaaatataaa taaatctggt cttattgcta
     7981 taggtttaag agatgatgat gagcttattg gagtagaact tacagatgga aaacaagaag
     8041 tacttttagt aactaaagaa ggtatgtcta taagatttga tgaaaatgat ataagatata
     8101 tgggtagaac agcaatgggt gtaaaaggta taactttaag taaagaagat tttgttgtgt
     8161 ctatgaacct ttgcagtaaa ggtacagatg tgttagttgt aagtaagaat ggttttggaa
     8221 agagaacaaa tatagaagag tatagaagtc aaataagagc tggtaaagga attaaaactt
     8281 ataatatatc tgaaaaaact ggtacaattg taggtgcaga tatggtcaac gaagatgatg
     8341 agataatgat tataaattct gatggagttc ttattagaat aagagtcaat gaaatatcac
     8401 tgtttggaag agttacaagt ggtgttaaat taatgaagac gaatgatgaa gtcaatgtag
     8461 tttcgattgc caaaataaat attgaagaag aataggataa atatcctatt ctttttgtat
     8521 aatgtagtta gataaaaatt taaaattgaa aggagtaatt gttatgtgca ataaaagaag
     8581 ttgtaaaaaa ggtggatttt tattattagg agctatttta gggtttatat ttggtatgtt
     8641 ttttgcacct aaaaaaggtt ctgaactaag aaaagaaaca aaagagaagt ttaatgatgt
     8701 taaagaaaat ccaaaagaag ttctacatga aacatttaat gatgttaaag agagaataat
     8761 aaatttagtg gatgatgata ataatgaaga agatataaag atttcagaag aagatatagt
     8821 aataagtaaa agctttgatg atgagggaga tgttaactaa tgaatgcatg ggggtggcaa
     8881 attggagcag tgttagtagg aagctctgct cttatagtag caatttattt agctaaaact
     8941 ctaaacagca taaataaggt tgttgaaaag gcatataaga ttgtagatta taatgaaaga
     9001 catatacaag atattgttga aaatgcatca tcaatatcaa agggaataga tgatatagtt
     9061 tatgtaatta ataaaatatt gagtatagga aatatattta aaattattaa aaggaaatag
     9121 gaggttttta catgtctatg aatattgatt caaatttaga ttcacaaaat aaattttgga
     9181 atgtttgtct tgatggagaa cttgatgttt ctacagcaga taaactaaag gaacatttac
     9241 atgctttaat agaaaaaaat atgttagatg taaagattaa tcttaaagat ttagattata
     9301 tagactcaac aggcttagga gctatgatag gtgtattaaa aaaattaaaa ataaatgaaa
     9361 aagagatata catagtaaat cctaagagta acgtaagaaa gatttttact ataacaggtc
     9421 tagataaaat atttaaagtg gagggataaa ctttggcttg tgagactata aagatggaaa
     9481 ttactacaaa tccagattat gttagtataa taagattaac agcatctgga attgccaata
     9541 agatgggttt tccaatagat gatatagaag atataaaggt agcagtatct gaagcatgta
     9601 caaatgcaat aaaacacagt aaagatgatg tatttaatat agtatttaat attttagata
     9661 atgcaattaa tatagaaatt caagataatg gaaaaggata tgatgttagc tcaataagta
     9721 caccagattt agagaatcca aaagagagtg gtcttggatt gtttataatt aaaactttaa
     9781 tggatgatgt agatattgaa tcagaacata atcaagggac aaaaataaaa atgactaaat
     9841 atttaggagt tgatatttaa tgaaaaatgt agctaatgct acacattacc taaatatgga
     9901 tacaaaagag ctatttaatt tatataataa aaataaaaat gtagacatta gaaatattct
     9961 tattgaaaga catctgtatt tagcaagatt gttagctaag aagtatataa acaaaggtgt
    10021 tgattttgaa gatatatatc aagttgcttc attggcatta atttatgcta ttgatagata
    10081 tgatgttgag aaaggatttg agttctccag ctttgcaaca ccaactatag taggagaaat
    10141 aaaaaagtat tttagagata aagtatggac acttagagtc ccaagaagaa tacaggaatt
    10201 aagcaaaaag ataagtgatg cgaagattaa attagaacaa gaaaataaga agcatccaaa
    10261 ggtaaaagat atagctgatt atataggcgt tagtgaagaa gatgttttag aggctatgga
    10321 ggcttcttat ggatatcaac caatgtcttt ggattcatca agtaatgatg attcagagga
    10381 taaagatata actcttattg ataagattgg aaaagaagaa ggtaactttg gaagtataga
    10441 gtatgaagat tttattaata agtttattga aactttaaat gaattagaag ttaaaatttt
    10501 taaagataga tttttctttg ataaaacaca atctagtata gctaaagaac tagggatatc
    10561 acagatgact gtttctagac tagagaaaaa ggtagtagag aagctgaaaa aagaatatga
    10621 aaaaaattta taaaaaaata taaaaaaagt attgacctac tgttttaaag atggtatagt
    10681 attacttgtc cttgaaaaaa ggaacaagaa ataaacacga acattgaaaa ttaaacagta
    10741 ggttaattta tat
//
LOCUS       DACULQ010000130        10675 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.131, whole genome shotgun sequence.
ACCESSION   DACULQ010000130 DACULQ010000000
VERSION     DACULQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.131"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            151..1851
                     /locus_tag="JA154_13690"
     CDS             151..1851
                     /locus_tag="JA154_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996896.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            1991..2638
                     /locus_tag="JA154_13695"
     CDS             1991..2638
                     /locus_tag="JA154_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996897.1"
                     /translation="MQIILQGECTMTNMEKEILLLISRVRCVTESQFRKIYGDQKRYN
                     KKNFKKTLRKMCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSEL
                     VVRINTAGCDVKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYEN
                     LSKKIDKSTIPFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            2809..3048
                     /locus_tag="JA154_13700"
     CDS             2809..3048
                     /locus_tag="JA154_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996898.1"
                     /translation="MMKGYIAEEILLTDKFSGLMQNMHKAVTFKVVSYKVTWSMKFTR
                     PKYTFGRFYLSKFSLRGRKHEYNCSKIWWKFSSRY"
     gene            2999..4192
                     /locus_tag="JA154_13705"
     CDS             2999..4192
                     /locus_tag="JA154_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAT7996899.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4210..5502
                     /locus_tag="JA154_13710"
     CDS             4210..5502
                     /locus_tag="JA154_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAT7996900.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYVEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            5719..6378
                     /locus_tag="JA154_13715"
     CDS             5719..6378
                     /locus_tag="JA154_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996901.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNSNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6533..7618)
                     /locus_tag="JA154_13720"
     CDS             complement(6533..7618)
                     /locus_tag="JA154_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAT7996902.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            7809..8525
                     /locus_tag="JA154_13725"
     CDS             7809..8525
                     /locus_tag="JA154_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAT7996903.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            8583..9161
                     /locus_tag="JA154_13730"
     CDS             8583..9161
                     /locus_tag="JA154_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7996904.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            9555..10427
                     /locus_tag="JA154_13735"
     CDS             9555..10427
                     /locus_tag="JA154_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAT7996905.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         4414 a         1115 c         1854 g         3292 t
ORIGIN      
        1 acatacacgt ataatgttaa atatagttga ataagcagaa ctataaatat ataaagatat
       61 cttaatatat aataaatatg ataaatattt attttttgta aatatatgga tataatagta
      121 ataataacat tttttagaga ggagtattaa atgaaacgat atcttaaaac atatggagtt
      181 gcattaataa ttgtaataat ttatgcattt attaaattac ctgtactaag attagatttt
      241 acatctttat tttcagtagg aataattttc tttgttgtag caggaatact agatatgatg
      301 ttagatagaa atgaaagagc atctaaaatg gctaaatata atttttcaat agcaatagct
      361 ttgcttgtgt atattgtagt agttcccttt ataacttcta cacctgtact tcatgcaaaa
      421 tcatatagag aactattggg aaaagtaact gaaagtaaat ttactgatga cattagccct
      481 gtcagtgtaa atgacattcg tcttgtagat gaagatatgg caatgaaatt aggtgacaaa
      541 aagcttggag aagttcctgc aatagggagt gtatccaaat taggaaaatt tcatatacaa
      601 aatgtagatg gagagttgta ctgggtagcg cctcttgttc acagagacat aattaaatgg
      661 ataacatctc taagtggtac aagtggttat gtaaaagtat cagctagtaa tcctcaagac
      721 gtccaattag ttcaagaaat agatggaaag ccaataaaga tagtttacca accagaagcc
      781 tacttacatc aggatttaca aagacattta tacatacatg gtattgtaaa tgttggtatg
      841 acagatttca ctcttgaaat aaatgatgaa ggaagacctt attgggttgt aagtttatat
      901 gaacataaaa ttggctatgg aggagcaaat gcaacaggaa tagccactgt tgatgcagaa
      961 actggaaaaa taaatgttta tgatgttaaa aacacgccta aatgggtgga tagaatacaa
     1021 ccacaaagct ttgtaacaga ccaaattaaa gactggggtg tttatgttaa tggattttta
     1081 aactctgtta tatcagaaaa gggagtttta gtaccaacag aaggtacatc actagtttat
     1141 ggcaatgata acaggtctta ttggtataca ggtataacat cttcaggtgg agatgaatct
     1201 acaattggat ttatgttagt tgattctaga acaaaagaag ctagattata taaacaacca
     1261 ggagctactg aaacggctgc aatgaaatca gctgaaggta atgttcaaga aaaaaattat
     1321 gaagcgacgt tccctgtaat gtataatata ttgggtcagc caacatatgt atcatcatta
     1381 aaagataagg ctggtcttgt taagagagtt gcatttgtat ctgttgaaga ttataatgta
     1441 cttggaatag gtgaagataa gaatgaagca ttaagaaact ataaggatgc attagaatct
     1501 aagggtaatg atttaaaact tgataatgat ttaaaagatg aggtattaga aggcacagtg
     1561 acaagaataa gcccagatgt gcgaggtggt aacactaatt attatgtaac tttagattca
     1621 aataaagata taatatttag ggctacttca aaagtatcta gcgaattgcc attgactcag
     1681 gttggtgata aagtcaaaat atcgtattca aaagaagaat ctggtgtaat agaaatgtca
     1741 gaatttgata acctaaatat tgcaaacttt gaagataaaa aagcaaaaga aaaaacagga
     1801 actgatgaaa atacagattc caaagattct gaaaatcaag gtaatgtata atttaaataa
     1861 aaagattgat aatagtattt aacatagtga tagctaattt ttatagaatt taattctata
     1921 aaaattagct attttatttt aattctttgt aaatttaatg tcataaaatt aatataatat
     1981 tagtacaact ttgcaaataa tacttcaagg agaatgtact atgacgaata tggaaaaaga
     2041 gatattactt ttaattagca gggttagatg tgttacagag tctcaattta gaaaaattta
     2101 tggagaccaa aaaagataca ataaaaaaaa ttttaaaaaa actttgagaa aaatgtgtag
     2161 tgaatataca ctaaaaaaat atccttgtga tgtggattat tataattata aagacctctc
     2221 ttatgtatac tatttaaacg gtagtaaact ttttaaggga gatgatttaa taaaggctct
     2281 agtaggatca gaattagttg ttagaatcaa tactgcaggt tgtgatgtaa aaagatttta
     2341 ccgaaatgtt agtgtagatg atgtgaaata cgatttattt attgaatata cagaccaatt
     2401 tggtaatata aagcaagttt tagttgatgt tgacttaaat aatgaaatag acatttctaa
     2461 atacgaaaat ctttctaaaa aaatagataa atctacaatt ccatttttta aagtaccaaa
     2521 cgttttggta gttacaccag ataatctatc cgaattaaat gatttagaga gatatgataa
     2581 tgagtatagc ataaatttta tagatttagg attaaataaa ctatttaatt gtatataagc
     2641 taactttaga aaagaaaact atcgatttgc cacattttgt aaaataattg ttgcaatatt
     2701 atatagaata tgataaaact atattaataa attaattaaa aataatgagc ctagggtaga
     2761 ggtagctgta ttcaatagta ttcaatagga gctggtaagc gttgataatt gatgaaaggg
     2821 tatatagccg aagaaatttt gttgacggac aaattttctg gacttatgca gaatatgcat
     2881 aaggctgtca cttttaaagt ggtgagctac aaggttactt ggtctatgaa atttacacgt
     2941 ccaaagtata cctttgggcg tttttattta tcaaaattta gcttgagggg gagaaagcat
     3001 gagtataatt gttcaaaaat atggtggaag ttcagtagca gatactgaaa aaataaaatc
     3061 aatagcagaa aatataatag aacgaagaaa agaaaatcca caaatggtaa tagttgtgtc
     3121 tgccatggga aaatccactg atgaatatat aactttagca aaggaattga gtaatgaacc
     3181 tagtaagagg gaattagatg ctttaatgtc tactggtgaa atgatttcag catctttatt
     3241 atcaatagca ttaaatgcac ttggatgtaa agctataagt tataatgctt accagttaaa
     3301 tataaaaacg agtggtcttc atggaaagtc tcaaattgat gatataaacg taaacagaat
     3361 taaaaatagt ttagatgaag gaaatgtcgt gatagtaact ggatttcagg gaatcaacga
     3421 agatggtgat gtaaccactc ttggaagagg tggttcagat acatctgctg tagctcttgc
     3481 ggttaaatta aatggaaaat gtgagatata tacagatgtt gatggaattt attttacaga
     3541 cccaagaaaa tattcaaagg ctagtaaact tgatgagatt gaatatgaag aaatgcttga
     3601 gttagcgagt ttaggagcac aagtaatgca ttcaagaagt atagagcttg cacaaaaata
     3661 tggtgttgaa atctatgtcg gacgtacatg tggtacagaa aaaggaacat atataagagg
     3721 gggaaaagac atgaaattag aggataaagt aataacagga ttagctacta gtgatgatga
     3781 cagttctata acaataaaag attttaaagc tgaaaatata tctagcttat ttgaagatat
     3841 agcaactata ggtataagtg tagatatgat aagccaaact gcacctattt tagataagat
     3901 aagtgtatca tttacagttc caaaagaaga gttgggagaa tgtaaaaaga tagtatctaa
     3961 atatacagat gaagaacatg tagtgattga taataatatc actaaatttt ctttagtagg
     4021 tcttggtatg aaaaatactt ctggagttgc tgctaaagta tttaagatat tcaatgaaaa
     4081 tggtataatg ataaaactta taactacatc agagattcgt ataacttgtg ctataaattc
     4141 tgatgataag caagtagcta tagaaaaaat agcagaagta tttaatatat aaaatttggg
     4201 gaggctatta tgaaacttca tggaactatg actgtaaata atgatgaatt atatattgga
     4261 ggagtgagtt gtttagaact tagtaaaaaa tatcaaactc cactttatgt atttgatgaa
     4321 gctttagtta gacaaaattg tagagaatat gtagaatatt ttaaggctag agagggaaaa
     4381 aataaggttg cttatgcagg gaaagctttt ttacctcttt acatgtgtaa tcttataaat
     4441 gaagaacaaa tgtgtttaga tgtagtttca ggaggcgagt tatatacagc ttataaatca
     4501 aattttccta tggaaaaagt tttctttcat ggaaacaata agactaaaga agaaataaac
     4561 atgggattag atttaggtgt aggtaaattt gtggtggata atttttatga attagatttg
     4621 cttgaagcat tatgtgaaga taaaggtaaa actcaagaga tatactttag aataactcct
     4681 ggtatagaag ctcatacaca tgattatata aaaactggac agatagattc taaatttgga
     4741 tttgctttag taaatggaga tttatttaga gcaatagaaa aattaagtga atataaaaat
     4801 attaaacttg tgggtcttca cgctcatata ggttcacaaa tatttgatgt agaaccatac
     4861 atagatacag tggatataat gatgaacttg gtaaaagaaa taaaagaaaa attcaacata
     4921 caactaacag aaattgacct aggtggagga gttggcgttt attatagcga aggagataaa
     4981 cctaaaacta taaaagagtt ttgtgaaacc ataatcacta aagcagaaac ttcatgtaaa
     5041 gagcttggaa tagaagtacc tacattggta atagaaccag gaaggtcaat agttgcaaat
     5101 gcaggaagta cattgtatac agtaggttct ataaaagata taaaagatgt tagggtttat
     5161 gtaagtgtag atggaggtat gacagataat gtaagacctt cgttgtacca agctagttat
     5221 gagtgtagta ttgtgaataa aattaatcat gaggctatga atcatgtaac aatagctgga
     5281 aaatgttgtg agagtggaga tatattaatt ggagatataa acataatgga tattaaaagt
     5341 ggagatatac ttataacaac atcaactggt gcatatggat attcaatgtc ttcaaattat
     5401 aataaaatac caagagcagc agtagtatca gtatttgatg gaaatgataa gataatttgt
     5461 aagagacaaa gctatgagga cttattagct ttagagatat aaaatggaga agtatataat
     5521 aaaaattaaa tattaaagtt tattatgagc agatttgcta ggatatgtat ctctaaatta
     5581 atttttggtt tattgtatcg ttcgtagcat atctgcttat tttttgtttt actaacaaat
     5641 ttcttgtaat aaatataaaa tattttaaaa actttataat tatgataaaa tttaaagtat
     5701 atgacaagga gggatttaat gagaggaaga gtaattttat taatatcgat attatctata
     5761 tttcttgtag gatgttcatt taataaaaaa gatgaaataa atttagtaga gcaaggaaaa
     5821 acatatttag aaaaacataa ctataaaaaa gctatggaat cattatcctc tgcactggaa
     5881 gaagatagta ctaatgagaa tgcaagagct atgtatatgc aagctatgag aatgtcaaat
     5941 atgacggagt ttgaagaatt aaaaaattat gagggtgcaa tcaaggaact taaacttata
     6001 gagaaaatca aaaatggttc ctctgttatt aaagaagagg caactaaaaa gaaagaagag
     6061 ttaactaaat tagaaaaaga acaaaacgaa gcagaggaac aaagaaagaa aaaggcaaaa
     6121 gatacggctg gagaagatag gtatagagtt gaatcagatg caaggaaatc ttcttatagt
     6181 ggaagtagta aaaataataa atctagttct aaaaataatg ataaaaaatc agatagtaat
     6241 aagtctgaca atagtaacag ttcttctcaa aatcaagaaa agccaaataa tagtaatcca
     6301 acaccagcac caactccaca agatactact ggtggttcag atagtggtgg aaattctcaa
     6361 aactcaaata atcaataagt ataaaatata tgcataaatt taatgaacaa attatatatt
     6421 ataaaaaata tctcttagca attttttctt actaagagat attttttgag aaataattta
     6481 ataaataaca gttaaatttt aaactaaaaa ttaaagtcac agtagagata agctattgta
     6541 aacgattatc tatatattta tcaacataag tttttacaac agcagctatt ggtactgaaa
     6601 gaaaaactcc tatgaaacca aatagagatt gtcccaccaa aatagcagat attacccata
     6661 tagctcctat accaacttga ttacccataa cttttggttc tataaaattt ccatctaatt
     6721 gttgcaatat aaatagaaat gcaatgaccc atatagtttt ttgtggacta tataatatag
     6781 tcatggctat tggagggatt gcaccaataa aaggaccaaa atagggaatt atattaccta
     6841 agaatattat tgaagctaaa aataaagcat tttctattct gataacaaat tgaaatccta
     6901 aaaatgcaat tataccaata ataagagagt ctaatattct accaagtaaa taatcataga
     6961 aaatatcatg agtagtccta aaaaattcta taatgttatc tgccttgttt ctagcagtag
     7021 aagaatatag aaatctctta aatcccaatg caattttttc tttgtcatat ataatgtata
     7081 tagatagtat aaatcccatt gtcatattaa atagcataga agatatacta agtgtataga
     7141 ttaagaagtt tttaggaatt ttaccaacaa aattacttgc ttcttttaat aaattatcta
     7201 aactatgttg tatatgagga attaaagttt ttagaaaata tatgtttgac atattttttt
     7261 ctataaaact atctatataa tcagtataca taggcatttc atttatcaat ctatttaatg
     7321 tattagctat tgatggaatt aataacctaa tggcaaaggc taaaataaaa cctataaata
     7381 tataagataa aaaaatggct aaaagtctat gtgctttgaa ttttatctca aaaagcatca
     7441 caagtggatt taaaagtaaa gcaagtaaaa tagctaataa aaatgggcta aaaaacttta
     7501 agaatccttc aatactagat ataaagtctg atggagtatt aataaattta aaaaataaaa
     7561 ttccaataaa cacgactatt aaataatatt ttatattttc ctttgaaata ataatcactt
     7621 ccttaaaata gtgtttagtc agctattaca tattaatagt atgtgaaaag aaattacaat
     7681 tatttaggaa aattaaataa tttttttgct tgtttttaat taaggtctag tatagtataa
     7741 taatgtagca aaattaacat tgataaaaat attaaacaca cataatttaa gataaggaag
     7801 gattaagtat gagaattaat aagtatatag cttcttgtgg aatagcatca agaagaaaag
     7861 cagaagaaat aattcttgga ggaagaataa aggtaaatgg aagtgtagta aaagagttat
     7921 cttttaatat tgatgaagaa aaagacatag tagaatttga taataaaaaa gtaaaaccaa
     7981 gtgaaaatta tatatatata gtattaaata aaccagaggg atatataact acagtaaaag
     8041 accaattcaa tagacccagt gtaatagaca tacttaaaga tgtaagggaa agagtatatc
     8101 caatagggag attagattat gaaactagtg gtttattaat acttactaat gatggcgatt
     8161 taacatataa gcttacccat ccaaagcatg aaattgataa aatgtatgtg gcaagtgtga
     8221 agggaattat aagtgatgat gagataagac aattcgaaac aggactaaaa atagaagatt
     8281 acacaacggc acctgccaaa ataagggtaa ctaaagaaaa taaagaaaaa aattattcag
     8341 tgtgtgaaat aactatacat gagggtagaa atagacaagt aagaaaaatg tgcaaagcta
     8401 taaatcatcc agttttaaat ttaagaagga tttctgtggg gaaaatagta ttaaaagaca
     8461 ccaaagttgg agaatataga tacctaacag aagatgaaat aaaatattta aaatcaataa
     8521 aataagataa aaataaattg agaaaaaatg aaataaagtt aatagtaggg agaaatagta
     8581 ttatggcata tgcaaagtat ttaactaaaa ttacagatat aaataaattt tatttagaaa
     8641 atattataaa tgaaggtgac atagtaattg atgctactat gggaaatggg tatgatacaa
     8701 ggtatctagc tgagaaggtt ggagaaaagg gctttgtata tgcttttgat atacaagaag
     8761 aagcaataaa gtctacaaga aagaaactag agaaagaagg atatacagat agagtaaaat
     8821 tgatatttga tgggcatgaa aaaatgaatc aatatataaa agaagaagtt tcatgtgttt
     8881 tatttaattt gggttatctt ccaagagcga aacattcaat tataaccaaa ccagatacaa
     8941 ctttaaaggc tataaagtta agtttagaaa tgttaaaaga aaatggagta ataagcattg
     9001 ccatatatac agggcatgat ggaggtcaag aagaaaaaga ttgcatatat aaccttgtca
     9061 ataatttaaa tcaagatgaa tttaatgttt tagaatctaa gtttttaaat caagtcaata
     9121 acccaccaca attgatttta attgaaaaga aaaaagcata ataaatacta aaaaacttga
     9181 caagattgta ttaagagtta atatatatag tgtgacctat taaaaatact ttaaataata
     9241 agtatacaac agtttattct caaaacaaaa tatacatcta aatatgcaaa ataagataga
     9301 taacttttaa atgagtaaaa attgaaaaat ttaaaagaaa tattggataa atattaaaaa
     9361 atgcaaggaa agatggtata ataaatttaa tcgaaaatac tatgtgaagt ataaggtaca
     9421 gaaaagttag aattttatga gtttaagaag ctatgataaa aactaagatg aaattgagat
     9481 aaaaaataaa atttttagag aaaaagacag tgttaataaa acaaaatagt ttatataact
     9541 aaaaggagaa aaaaatggaa gaaacaaatg aaatgaagga cagtaaacat ttgatatcaa
     9601 atatacaatc tcaatatacg agattgagta agggtcaaaa acttatagca caatacattt
     9661 taaataatta tgataaagtt gcatttatga ctgcatgtaa attgggtgaa actgtaggag
     9721 taagtgagtc tacagttgta agatttgcaa atgcattagg ttatagtgga tatcctaaat
     9781 tacaagctgc acttcaagaa ctcataaaaa ataaactaac tacagtacaa agagttgaaa
     9841 tggcacatga ttattccgat gattttgcaa tactaaacaa agttttaaaa agtgatatag
     9901 ataatataag gtctacacta gaagaaattg acgagagagc gtttaaagaa gcttctaaca
     9961 aacttttaag agcgagaaaa atatatatac ttggaatgag gagttctttt gtagtagctc
    10021 aatatttagg tttttactta gatataatac ttgacaatgt tcatataatt agaatggata
    10081 tgggagatgc ttttgagcag attgtaagaa taaatgaaga agatgtaata gttgctatta
    10141 gtttccctcg ttattctaag aaatcttatc aaattgtcaa ttatgctaaa gaaaaaggag
    10201 cccatgtaat atcattaaca gatagcttat ttgcgccagt agcatcactt gcagataaca
    10261 ctcttctagt taagagtaat atggcttcat ttgtagattc tctagttcca gctcttagta
    10321 tttcaaatgc tcttgctata tctgttggga tgaaggaaaa agaagatatt aaacagcatt
    10381 ttgatgattt agagcaaatt tggaaaagat attctgtgta tgagtaaagt ttgtgtatta
    10441 ataaggctat gaaattgaga atcatagcct tattaataaa tactagatta tcacttaaaa
    10501 aataaatttt cgtttaaata atacaaataa atatttcgag actcaaaaca taaagatgta
    10561 ttctgaatat atctttacta catcaactaa tttattaaag aaagcatttc ccttcatgga
    10621 gagtgctttt tttatttctc taaacaatat catttcctta attaacaatt ataaa
//
LOCUS       DACULQ010000131        10652 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.132, whole genome shotgun sequence.
ACCESSION   DACULQ010000131 DACULQ010000000
VERSION     DACULQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.132"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            148..1308
                     /locus_tag="JA154_13740"
     CDS             148..1308
                     /locus_tag="JA154_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996906.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1651..1812
                     /locus_tag="JA154_13745"
     CDS             1651..1812
                     /locus_tag="JA154_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996907.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2005..2634
                     /locus_tag="JA154_13750"
     CDS             2005..2634
                     /locus_tag="JA154_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAT7996908.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMGGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2874..4133
                     /locus_tag="JA154_13755"
     CDS             2874..4133
                     /locus_tag="JA154_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance protein"
                     /protein_id="HAT7996909.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4413..5387
                     /locus_tag="JA154_13760"
     CDS             4413..5387
                     /locus_tag="JA154_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HAT7996910.1"
                     /translation="MNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIKVIKHNNY
                     ICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGIGLDELIL
                     GNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDFGITKANN
                     GVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNSEEKMLQS
                     IDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKAYIEVEDE
                     KEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5633..6700
                     /locus_tag="JA154_13765"
     CDS             5633..6700
                     /locus_tag="JA154_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAT7996911.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYNKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7234..9144
                     /locus_tag="JA154_13770"
     CDS             7234..9144
                     /locus_tag="JA154_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAT7996912.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9207..9830)
                     /locus_tag="JA154_13775"
     CDS             complement(9207..9830)
                     /locus_tag="JA154_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996913.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10018..>10652
                     /locus_tag="JA154_13780"
     CDS             10018..>10652
                     /locus_tag="JA154_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7996914.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLK"
BASE COUNT         4356 a         1053 c         1751 g         3492 t
ORIGIN      
        1 taatatatgt caatagttag gtatatatgt atttaattag catgatatag ataaaagtat
       61 ttattttaat tgtctattat ctgaaaaagt ctttacttag aaatcataaa aatcaaatta
      121 attaggaaaa gaaagggtgt gtataatgtg attgagactg gatttgtata ttgtagtttc
      181 ttggttgtat tcgtaggtgt aatttttttt atggttgata aattgaaatg gaaggtattt
      241 gatgttatta atccaatgct tttaatatac ttaggttcag ctatacttgg atcatttggt
      301 ttatttgcac agaatgaaga tgttggaacg tatcaaaaca taataacagt gaacttcctt
      361 ccattaatgt tagtgttttt gttaattcaa tgtgatttaa gaaaaatatt aaagatgggg
      421 cctaaaatgt tattatcatt tttctgtgct acatttacat ttatgcttgg atttgttata
      481 gcattcataa tttttaaaaa ttcaatgggt gcagattcat ggaaaattct tggggcagca
      541 tctggttcat ggataggtgg ctcatcaaat atgattgcag cagctaatgc tctaggagtt
      601 gcagaagaag gacttagtta tgcaatactt atgggttcta taggatatac agtatggatg
      661 tctgtttgtc taatgtctgt gaaatttgga cctaaattta acaaatggac aaagtgcgat
      721 acaagtttta tagatgaggt tacatcaaaa cttgaaaaag aaaataaaga taatacagca
      781 cctacgtttg tagaacttat gattttactt tctgttgggt ttggagttgc gtcttttgca
      841 caaattgtat ccaagatact tccaacttca ggtatactta atgaaactat gtgggtagta
      901 cttgttgcat ctacagtatc aatattattg ggtatgacaa aaatatcaaa gcttaaaggg
      961 agtacactgg taggaaatgt gtttatgtat ttactccttg cagctacagg tagtaaagca
     1021 aatttctttg gattgtcaga agcacctgtt tacataatgt ttggattggt agtagtcctt
     1081 gtacatgcta tttcatttat agggcttgct aaactattta aacttgattt atttacttgt
     1141 gaagtaggaa gtatagctaa tataggagga gtaactacag ctccaatgat tgctggggtt
     1201 cacaatcctg tattaatacc agtaggtgta cttatggggc ttctaggaaa tattataggg
     1261 acttattgtg ctcttatggt aactcaaata ttacatatgt tagcttaatg aaaatatggt
     1321 ataaaattaa taatttaaat aagaaaagtc atttcaattt aagcagaagc ttttactgaa
     1381 atggcttttt ttgtttgaca agataatcta tttcaagaag atgttttgac atatttagtg
     1441 tataatattg taaaatttac taaaaacgag atatatttaa tttttatatt gaatcgaacg
     1501 tctgtatgat gtataataaa tatacagaac gaatgtttgc ggaacgggtg ttttttgaat
     1561 ttattttaat aatttatgaa tatatatcag aatgcataaa tatatttaaa tataaagatt
     1621 aaaaagaatt taaaggagtg ttgataactt atgataattc catcaagaag aatacaagat
     1681 ataacattaa caaatttaaa aaatggtgaa gtaactttaa ttgaattgga tgaaatatac
     1741 aagaagatgg gatttttatt tgttgttagt gaaggcaaat tgaaaaagat aaaaaaggag
     1801 aatcatcatt agaaatgaat gaaataataa atattataat agtttataaa acctaactta
     1861 tgtagaatat aaatacttaa gttaggtttt tttgtgataa agtttataag tactcaattt
     1921 tataagataa ataatggttt taagtgaaaa gtaaataagt ttaaatttta gtaagtatta
     1981 tattgcataa gaggtgatat tattatgaaa atagtaacct acaatattca taaaggtatg
     2041 gattcaaata atagattaac tctaactaaa atggggttat atttaaaaca attggattgt
     2101 gatgtaattt gtttgcaaga agtattatat cctcaatttt tagctttaaa agctgtttta
     2161 aatatgggtg gtgtctttgc tacaaatgta aaaaaagtaa atatgatata tggtatttgt
     2221 acatttacaa catctaaaat gttaaacagt aatcattttt tcttaactag caaaaaagaa
     2281 caacggggag ctctttgtat aactatagat gcatatggaa gaattataaa tgtaataaat
     2341 actcacttgg gacttgatag gcaggagaga gcaaaacaat tagacgaaat aatagactat
     2401 aggaacagat tagtggggat agttatttta tgtggtgatt ttaatgagaa aaatgtcttt
     2461 ttaagtatgt ttaatgatat ggctatttca ctaaacaagt cttacttacc aacatttgaa
     2521 aaatcaaact ctagaataga ttacattttt gttaataaaa atactgaact aaaaggatac
     2581 acagtagaaa aaatttacct gtcagaccat tatcctgtaa taggttacat ttaaatttca
     2641 atataaatga aattagttgt tataaaataa acaatttttt tgttaagaaa tagtaaaaga
     2701 aatataataa aaaatatcaa aaataaaact aattataaga cataaattta caataaagtt
     2761 acatttgatt aaattccttg tttaacaaaa gctattcaaa tataatctaa ggtagttttg
     2821 aaatgggaac tctattatgc aaatttaagc tagtgttaaa atgaggtgaa gttatgggca
     2881 gaagaacaac aagagtttca agattaggta aagaaaaaaa acgaagtgtt ttaattgtat
     2941 tggcagcttt agttctaata atgggtggat ttacttattt ttttaacagt aaatttctgt
     3001 acaatgggaa aatcgctaaa aatgtatata tagaaggagt aaatgtatct gatatgacta
     3061 aagcagaggc attaaaagct ataacagata agtatactcc agaagactta aatcttgctt
     3121 atgatgggaa aaaatataca atatcaccaa aagatattga tttaaaatat gatacagaag
     3181 atgttgtaaa agatgcttat gaaagtacaa aaaaagggtc ctattttcaa aacttaaaaa
     3241 aatatattga tataagagtg aataaggcaa atatgaagat aaaagctgaa tatgatgagg
     3301 caaaacttag ttccaaagtg tcttctatag cagatagtat aaatgttaag atgaaaaatg
     3361 cttctattag tgttggaagt ggtggactaa gttatacaga ctctgttgta ggtagagaat
     3421 ttgatttggc agcaaataaa gagtctatat ataatatgat aaaaaataaa gagcataaaa
     3481 ctttagaact taaagtaaat cttcaaaaac cagatataac aactgaacaa gtaaaaactg
     3541 taaattctgt tataggtcaa tattcaacta cttattcaca agctgtagag ggtaggtcat
     3601 ataatgtagg attatcagca agaaaaacta gtgatgtatt attaatgcca ggtgaagaat
     3661 tttcatacaa taaacttaca ggacctagta ataaagcaaa tggttacaaa gatgcacctg
     3721 ttatagttta tggtaagcta gaacaatcag caggaggagg tgtttgtcaa acatcttcaa
     3781 cagtttataa tgctgctctt ctttcgggta tggagataac tcaagttaca aatcattcat
     3841 ctgcatctac ttatgtgcct aaagggagag atgctactgt gagcgatgga ggattaaact
     3901 taaagtttaa aaatccatat aagcatcctg tatatattaa aaattatgca ggtggaggaa
     3961 gtgtatcatc agtaatctat ggtaattctg gtgacaaacc aaatatatct atagaggtaa
     4021 aacaaactgg tcagaataaa tattctacat atcgaatatt taaagattca agtggaaagg
     4081 tcataaaaaa agaacatata tcaaatagtt cttataaaga attaaaaaaa tagataattt
     4141 tttagagaaa tatttgtaat ataaaaggga gtgtctcaaa atgaactttt tagtttatga
     4201 ggcactcttt ttctgtgaaa tcaaatatat tttcatcatt tcaacaatac aaaagaagcg
     4261 tatctctatt ttgagacagc cttttttgta aaaattaaca ctattagttc ttgttttaat
     4321 attacaatca ttaatatata gaaaatattc ctatatattg gtataattgt aaatagattt
     4381 agttatataa aatgtatgag gaggtgacat ggttgaattt tataggcaat agatatgagg
     4441 tagtaaattc tgatgacgtt ttagaaatta acaaaatcta taaagctaga gatgtttttt
     4501 acaggaaaaa tgtgttaata aaagttataa agcacaataa ttatatctgt gaagattttg
     4561 tttcaaattt aattgatgaa agtactgctt tagatgagat aaactctcct tatatactaa
     4621 aaataataga tgttgggatt cactgtacag aagaaacaac attatattat gttgtaagtg
     4681 aagactttaa tggtatcgga ctagatgaat taattctagg taattatctt catttagaag
     4741 caattgtaaa cataatgata caagttttaa aagctttaga aatgatacat aggaatcatt
     4801 catatcatgg tagtttaaaa tcgagtagta taatagtaga tacagaatac aatataaaaa
     4861 tttgtgattt tggaattaca aaagcaaata atggggttaa tacaagaagc tatggaaaca
     4921 ggagatattt atgcccacat caactatgta taaattatac tgataaagaa agtgattttt
     4981 ttgcaacagg tcttattcta tttgaatcta tatttaaaaa attgcctttt ggagaatcaa
     5041 attcagaaga aaaaatgcta caatctattg ataagggatt agattggaat agtataaaag
     5101 caattaatgg aaatactgaa cttataaatg ttataaaaag gcttcttgga agaaataata
     5161 agtatactag agccaatgat attattattg atttaagtaa agtaatgtat gaaaaagcat
     5221 atatagaagt tgaagatgag aaagaagaag agcagaaaca agacaaagtt atacaagtta
     5281 aaaaagaata taaaagtaaa atgagaaaaa aattacataa aaaacttgtt gttgccggtc
     5341 tagctatagt catgatttca atgattatta ttagttccat aatataaaaa ttgtatatta
     5401 agtattttaa aatcaatgtc tataaaggat atacatagaa attttagtgt agtcttaaat
     5461 tatgaaagga agtggttatt atgttaacaa aaaataatga ggtattaaac gtctaggagt
     5521 attttaacaa atagccacct gcttttacag tttagaataa aaaagctgtg gctaaaggtc
     5581 acagcttttt gctacttttt aaaggcagga gaatattaaa aaggagcaaa ttatgagcaa
     5641 tataaaaata atagcaagta ataaatgttg gattgaagat atagcaagga aacaacttga
     5701 agatatttca aagttaaatg gaattttaag agtagtagga ttacctgact tacatgcagg
     5761 taaaatacct gtaggtttag cagttgagac taaaaatatt atttatccac atataattgg
     5821 aaatgatata ggatgcggaa tgactttatt taaaactggg gttctaaaaa agaaatttaa
     5881 aaaagacaaa tggattaagt cacttagtaa gattaaagat ttgtccgata tagaaataaa
     5941 aaacacatat aaagaagaat gtccaatttt aaatttagga actataggga gtggaaatca
     6001 ttttgtagaa attcaatgta taagtgaaat ttataataaa gaacaatttg aacaattaaa
     6061 attttcatct gatgatatta tgatgttagt tcactgtggg tcaagaaatt atggagaaga
     6121 tattttaaaa aaattttatg ataaagatgg acttgaagtt gaaagtgaga aagctataga
     6181 ttatattaaa aaacatgata atgctttaat gtgggcagag cgtaatcgtg aagctatcag
     6241 taaaaaaatt atgcagtcaa taggtacatc tggagatgtt gaaacaatat tctctataaa
     6301 tcataatttt atagagaaaa gggaagataa atttatacat agaaaaggtg ctgtttcaag
     6361 tgaaagagga gctgtgatta taccaggttc aagaggcagt ctatcatata tagttatgcc
     6421 tactgaaaat acagagatat ctttatattc tctatcacat ggagcaggta gaaaatggtc
     6481 tcgttcagtt tgtaaatctc gccttaaaag taagtataat aaagatacta taaaacaaac
     6541 taaatttaaa agtcagatta tatgtaatga tgtaaattta ttatttcaag aagctccaga
     6601 ggcatataag aatatagagc agattattga aagtttgata gaatatgaac ttattcaagt
     6661 agtggcaact atgaagccat tgattactta taaaggataa tttttaaagt taaaaaagat
     6721 taaaattata ttataaatag ctaattttat ggaacattta aaagtgtttt attattagct
     6781 atttttttat tttgtagaga cgataattta tagagctaaa ttctttgata tataatgatt
     6841 acaaagttag actgtattgg aaaatgcaca tacatgaatt gatgacaata taagtgattc
     6901 ccaaatataa atgaaagtat aagcttattt tcactataaa aattttcact gtacaaaaac
     6961 attaatgaca ttatttttat tgacataaat atataattta tggaataata ttagaagcac
     7021 tgatataagt gtttaaataa gaatagaaaa ggagaagtgt tttatgaaaa aaaatactaa
     7081 cattaatgag tccggagtta aacttcttaa taaataagga gtttgatata taataactcc
     7141 ttatttatta agttgttcgc aaaataacta ttatcaaagt aaatgatagg gtgatttaaa
     7201 agacgataac ttataaataa aggagaaaaa ataatgaata gtaatacatt tgaaagatta
     7261 caactaaatg aagttaaaga attaataaaa atacattgtg taagttcact aggaaaaaat
     7321 ttaatagata aattaactcc tagtgggaat attggagtag taagaaggaa attagcagaa
     7381 aataaagaag ctagaaagat aattgaaaat agtaatcaca taccacttga aggattgttt
     7441 aatgcaggtt ctacaataga taaaattgaa aaaggaatga taatagaacc tgttgaactt
     7501 gtaaatattg aagatttttt aagaggttgt aggaagatga aagcttttat gttagaaaaa
     7561 gaattttatt cacctacatt aagttcttat gccttaaata taacagaatg taaaagtata
     7621 gaagatgaga taaattactg tataaaatct aataaagttg attctaatgc tagtaaagaa
     7681 ttaaaaaaga taagacgaaa tatagaaatt acagaaggaa agataaaaga tagattaaat
     7741 aaatttataa catcaactgt aaataaaaag tatatacaag agtttataat aagtaaaaga
     7801 aatgatagat atgtaatacc aattaaatct tcttataaaa atgaagttaa tggtactata
     7861 ttagatacat catcaaaagg aaatacggta tttattgaac ctattagtgt ttcaaattta
     7921 agtacagagt taactatgtt aaaagctgat gaaactattg aagaatacaa aatattatct
     7981 tatttaacag aacttatatt tgagaagata agtcagataa aattaaatat agagatactt
     8041 tcagaatacg atatggtatt tgcaaaagct aagtatagtc aaaaaataaa aggaattact
     8101 ccaaaaataa ataataatgg ctatataaaa ataattaaag gtaaacatcc acttctaaca
     8161 ggagatgcag tacctttaga ttttgaaata ggcaaaaatt atagaagtct tataataaca
     8221 ggtcctaatg caggaggaaa aactgtaacc cttaagactg ttggtttact aactttaatg
     8281 gttcaatgtg gatttgatat ttctgcaaaa gaaggtagtg aatttagtgt atttgaaaaa
     8341 gtttttgtag atatagggga taatcaaagt attgaaaatg ccttaagtac tttttcatca
     8401 catataaaaa atattgcaga gattatgagt ttatcaaata attctactct tgttctgttt
     8461 gatgaaatag gaagtggaac tgaacctaat gaaggagctg gtttagcaat atcattatta
     8521 gaagagtttt acaaaatggg atgtataact atagcatcaa ctcattatgg agagataaaa
     8581 aagtttgcta cattgcatcc agaatttgaa aatgctggta tgatgtttga taaagaaacg
     8641 ttagaaccat tgtataagtt aactattgga aaatctgaag atagtaatgc tctttttatt
     8701 tctaaaaaaa tgggaataaa aaatagagtt ttagatagag ctaaagaata tgtaattgat
     8761 agaaactaca atttagattt agttaaaaga agtaagctac aatctaatat tgaagataaa
     8821 aatagttctg aagtcatttt aaactataca gattataatg taggggataa ggtaaaatta
     8881 ctagatgaag atgattttgg aattgtatat aagcctatgg acaagtttaa taatgtagaa
     8941 gtattgttta atgataattt tatttcagta aatataaaaa gattacaact tagcataaaa
     9001 gcatcagaat tgtatccaga agggtatgat ttagatgtat tatttactag ttttaaagaa
     9061 agaaaattag aaaaagatat tcaaagaggt tcaaaaaaag ctcttaaaaa gattcagaaa
     9121 gaaattagag agaatagaaa ataaatataa aaagcacaaa agaaagcaat ctaaagagaa
     9181 aagtttatct ttagattgct ttcttattaa catatcttca taaatccaat gactaacaag
     9241 attaaaccac ttaaccaaga taagtttaaa tttacgtttt taacaaacat atttcctagt
     9301 aaacaaccaa gtaatactgc aattatattt gatattaaag aaaatagaat aacttgtata
     9361 taattcacac ctacaaggcc actaccaaaa ccgatagcta atccatctaa acttaaagca
     9421 agtgcaagtg taaatgcttc agcagattta agcactttag atttacttgt atctaagaca
     9481 tcaggttcaa tacttacatt aaaatttaat ttaaggtcta acatattaaa attataattc
     9541 tttgagtgta tagatttttt cttaaaataa gatttaaaaa aactttcaat tagtctagca
     9601 cttcctaata aaaataaaat aaagaaacat attaacattg ttatattttg aggtaaaaag
     9661 tctctaacta ttgtacctgc aaaaagcgat attgctaaaa tagctgaaca aactacgctg
     9721 ataattatat ttgaatataa gggaacttta actttattta cgccatatcc aaagcttgtc
     9781 acaaaggcat ctatacaaag agaagtaact aataaaattg attcaatcac agtagattaa
     9841 catcctttct cttaaatttt gtacttatat tagttatagt attgtaaaaa tggataaatt
     9901 gttacagata atccaataaa ttgttgataa aattccaaca tcatacaaca cctaaataac
     9961 aaaagaatca tatcatataa taatataaaa ccaatcataa taaagcaggt gaaaataatg
    10021 gaacaacata gtaaggttgg gaatttcggt gatttgttga aagttttatt agatgaaaaa
    10081 gaattatcaa tgggagaact aagtaaaaaa agtggaattg ataaatctac tatatctcgt
    10141 atagctaata ataaacaaaa gccaaatata aatcatttag aaaagatggc aatacattta
    10201 aatattaacc tagaagaact gttgaaagct tcaggatatg aatttagaaa cagcaataat
    10261 caaatattta atgcagatag tgattttagt aactttgatg atattcttgg atttgcaaat
    10321 ttaatcaatg ataagaactt taatggaaat attgaaaaag agttaagtaa gtgtaagtta
    10381 tatgtccaaa cagatgaagg taaaaaatta ctttttgata attttaataa aaaaatagat
    10441 tctattgaaa aacaaggtca atttacagat agattaagac aaatgtatgc tgatttttgt
    10501 acagatggac ttacaataaa gaaatattta ttgattggaa gtatgctact ttactttgtt
    10561 atatcaacag atgtgattcc agacttcgtg ttcccaatag gatttatgga tgatttagta
    10621 gcattaaata tagttacaaa attacttaaa aa
//
LOCUS       DACULQ010000132        10543 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.133, whole genome shotgun sequence.
ACCESSION   DACULQ010000132 DACULQ010000000
VERSION     DACULQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.133"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            27..251
                     /locus_tag="JA154_13785"
     CDS             27..251
                     /locus_tag="JA154_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996915.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            304..828
                     /locus_tag="JA154_13790"
     CDS             304..828
                     /locus_tag="JA154_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7996916.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(1218..2144)
                     /locus_tag="JA154_13795"
     CDS             complement(1218..2144)
                     /locus_tag="JA154_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAT7996917.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            2334..4205
                     /locus_tag="JA154_13800"
     CDS             2334..4205
                     /locus_tag="JA154_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996918.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(4297..5232)
                     /locus_tag="JA154_13805"
     CDS             complement(4297..5232)
                     /locus_tag="JA154_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAT7996919.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            5752..6264
                     /locus_tag="JA154_13810"
     CDS             5752..6264
                     /locus_tag="JA154_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAT7996920.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(6419..7477)
                     /locus_tag="JA154_13815"
     CDS             complement(6419..7477)
                     /locus_tag="JA154_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HAT7996921.1"
                     /translation="MIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVIS
                     GVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEAV
                     KAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIVV
                     EGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSSS
                     DVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAIR
                     NKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAYK
                     IDKLSTVEDVINELISEL"
     gene            complement(7604..8497)
                     /locus_tag="JA154_13820"
     CDS             complement(7604..8497)
                     /locus_tag="JA154_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAT7996922.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(8507..9988)
                     /locus_tag="JA154_13825"
     CDS             complement(8507..9988)
                     /locus_tag="JA154_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAT7996923.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(10096..10327)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         3976 a         1354 c         1505 g         3708 t
ORIGIN      
        1 caattatata aggagtagat aaaaatatga taataaaact tactaaagac aattttaatt
       61 ttagatttaa acaatatgaa ggtatgtttt ttcatgctga agtagttgaa ggtatggaat
      121 ccattagata taaaataact aaggataaag aaggtacaga attaaaagaa tttggaagta
      181 cacatataaa agataatatg atatatgtac ctcaggtaga agcatacata gatttgaaca
      241 gtataaaata aagatgtaaa attttaatta aaaataaaga ttatgtttga gggggaatcc
      301 aatatgagta agcctataga tataacaata agacctataa gaagagaaga tgcacaagct
      361 tttaatgaaa ttagaagaac atacagtgtt atgagaaata ctttggcatt aatgagtgac
      421 agagtcgata aaactgaagc tatgttagct tctttgggag aaaatgatta tatgtttgta
      481 gctgaaacta ataataatgg agaaaaagat gtaataggtt ttgctgggtt acatgtaaat
      541 tcttctccac gattgagaca tagcgcagaa ataggaataa gtgttgcaga gaattatcag
      601 ggaagaggag taggtaagaa gcttatggaa caacttttag atatagcaga taattggatt
      661 atgttaacta gagtaggatt agaagtaatt gtggataatg aaaagggatt aaatttatat
      721 aaaaagctag gttttgaaat agagggaact aaaaaatatg ctgtaatcag agatggaaaa
      781 tttgaagatg tttatattat gggaagatat aataaaaatt taatttaaat aagctaatag
      841 agaagtaaaa tagagtgtct ttaataaaaa atttacatta aagataatct attttacttc
      901 tctattgtgt taaaaaactt atttttaaat gtttacaaaa acattctata tacaaataaa
      961 attattagca atatcaacgc tattttaaaa gctaaactag caacagtttt aatgattttt
     1021 acagaaacac ctatagcaac tatagcaaat actattttaa agagtaaatc catatccata
     1081 tttaagcctc ctattctata atacatatag atattattga caaatatcta ggaaaaaatt
     1141 ccatcaatat actacaataa aatgtttata tttgtaattt attgaatatg ttattatata
     1201 atacataaaa aaataaatca agataagagt aaaatactta taaaaggtat agatataact
     1261 gaaaatagtg ttgtaaaaaa cacacattgt gaagctagtg ttatattgga gtcatattga
     1321 ttagccatta tagcagtatt tgcagcagca ggcattgaag aaattactac tggtatagct
     1381 aaaatcatct tatcatttat ccatcctttt agaataaaat acacaatcac aggaagtacc
     1441 aataatctta taaaagtgac tatatataat tttttgttga caaaacaatc gagagctgaa
     1501 gaattagcaa gtaagcttcc aatgataatc atggatattg gtgtagttat acttcccaac
     1561 atctcaagtg gctcgtttat aaattggggt aacctaagtc ctgttacaaa taaaaaaagt
     1621 ccaataacaa tagctattgt agcagggctt ataagtgatt ttattgaaaa acctctactt
     1681 gtactgcctt tagataaaag ataaattcct aaagtgaaag aaaataagtt aaaaggcaaa
     1741 ttgaatattg cagtatagaa tattgcttca tgacctaaaa ctgcttcaat tacaggataa
     1801 cccataaaag caacatttga aaaaacaact atatactggt atattcccaa atctttttta
     1861 tcattacatt ttaaaatgta tttcaataaa aatgcaatta taattgaaac tatatacata
     1921 atcaaagatg taaataataa caatagtatt ttttgtatca ttttactatc aaagttaatt
     1981 tgcatggagc taattatcat tgatggtaaa aaaactgtca ttgtcaaagt tgaaagctta
     2041 gatgtgcagt ggtcatctaa taagttgaat tttctgacaa aatatccaac taaaataatt
     2101 ataaataaaa ctgcaacttg gataaaaata ttgctgaaat tcataaaatg tcccccctat
     2161 atatacatta tataccaatt acataataaa atctaatgat tataaaattt aaaatttaat
     2221 gttacaatat agtgacaaaa ggtaaaaatt caatattata gcattaattg tagattataa
     2281 tgtataataa tgtttgagag aatatatttt ataaaatggg taggtagcga ataatgaaaa
     2341 atacaaaatt ttctataaaa aaaataaaaa atatatctaa tgacccgaaa gtcgtacata
     2401 ctaagggtgc cctccttgaa aagatgggta agatggaaag cgcaatagag ttgtataaga
     2461 gtgctgcatt ggataattac gtaaaatctc aatatacttt aggaaatata tatgaaagta
     2521 aaaaacaatt taaagaagca gaaaagtggt attcaatggc atataaaagt ggaagtgaag
     2581 atgctgcttt tgatttaggt aatatgtact ataagttaga tggctatgaa tatgctatat
     2641 attggtatga aaagatagca aatttaggtt atttacaagc acaaaataat ttaggtgtat
     2701 gccattttaa aatgaaagat tttgtaaggt gtgaaaaatg gttaaaaaca gcagcagaca
     2761 agaatttagg taaagcttgt tttaatttag gggtattata tacttttttg gagaaagatg
     2821 atgaagcata tgaatactat aaaaaaggtt ctgtgctttt agatgatgat gcaaaatata
     2881 atttagcaat attaaatgga aaaaagaaag ataataaatc aacagtaagt ttatataaac
     2941 agttgtacaa gtcaggtcat atgaaaggtt gctttaactt gggtatgatt atggaaataa
     3001 atgataattt agaagatgca gagaagtatt ataaaaagag tgctgataga gatgatgtga
     3061 agtctgagta taggcttgca tatgtatatg atagaaaaga agagctggga gatgcaattt
     3121 attattatga gaaagcaata gaaaaaaatc atacactttc taagtataga cttgctaatt
     3181 tatataatag agaaaatgag atagagaagg caaaaactta ttataaaatg gctgctgaag
     3241 atgatataac agaagcaaaa aataatctgg caggtatata ttttgaagaa aaagattatg
     3301 aaaatgctat taaatattat gaggatgcta tagctgttgg atgcaaaagt tcccttgaaa
     3361 atctagggga tttatattat cagaatcaag atatagaaaa agcgatttcg tattattcga
     3421 ggattccaaa taatgtatct tgtcaaatca aattaggaaa tatctatgaa gatttaaata
     3481 atattgaaga agcaataagt tggtataaga aagcatctga aaatggagat acgaggtcta
     3541 gttatagatt ggggtgtata tatgagagct taggaaatac aaaaaatgct agaaagtatt
     3601 ttgaaatggc aagtagcaaa aaccatatga atgctcgtat acatctaggt agaatttatt
     3661 ttagagaagg taaattggaa gagtctaaaa tgatgtttga tacacctgcc aatgaaaata
     3721 atgtgtatgc tcaacatatg gtaggattaa tttatgatat gttctataaa gattatgtaa
     3781 attctaaatt ttggtatgaa aaggctagag cacaaggttg tgttgaatca atatataatt
     3841 taggtcaaat ttatttaaaa ttaaatgatg acgcagaagc tgagaaatat tataaagaag
     3901 gtattaaata tggaagtaag aagtgtgagt atatgcttgc aggtctttat tataaaaaga
     3961 gcctagatat gtacatttct ttagcaaatg aagagtacga taattgtgaa gagatagtag
     4021 aaggctttcc aaatttaaat atagactttg atgaagtatt aattcctcca tttaagttgc
     4081 aagaagaggt aatagatgaa gaagagtatg taccaattta tatactaaat ataaaagaaa
     4141 gtttagattc aatgctagaa gggcttgaaa ctgaaatgat tatagatgaa gaacatgata
     4201 gttaaaaagt attataagat tatatagatt taaatatata aaagccatta ggataaatgt
     4261 aaatcaatta attctaatgg cttttttatt aatattttat aatagcttat ttcttgacat
     4321 gaaaaatgta atcactatac atattataga ggtaataaga agtattatcc aaaatccgtt
     4381 tgggttatta gaaaatggca tgttgatgac attcatacca aagaatcctg atattattgt
     4441 aggtattgag aaaattagtg taactactgt taaaatttgc ataacattat tctgattatt
     4501 acttataatt gcactgaaag catccataat tctactcaat atatctccat atattttagc
     4561 catatcaaga gcctgtctat tttcaactat aacatcttct aataggtctt catcatctgg
     4621 atacttcttt ataccagcag ttctaaccat tttgtttaaa acaagttcat tagattttag
     4681 tgaggtagag aagtatacca aagatttttc taattccaat aattgaacca attctttgtt
     4741 tttcattgat ttgtaaattt ctcgctctat tatgattgtc attttattta tttttcttaa
     4801 gtaatgcaag taataagctg catttttgtg aagaatttga agcaaaaagc gagtcttttt
     4861 aaacgtaaaa aaatctttaa tatgaccaac tataaagtca tttaaaatct tagtttgtgc
     4921 atcacaaact gttacaatag cttcttcagt tagtataata cctaagggta ttgtagtata
     4981 atgagaagaa ttagagtcac tttcgtctac tggtatatct ataagtataa gagtatgatt
     5041 gtcttcaaca tctattcttg agcgttcttc ttcatctagt gcagattcta tgctttctac
     5101 atctatattt aataaattag aaatttcgtt gatttcacta tgattaggtt caacaagatt
     5161 tatccaacag ccatcttcaa aaaaactaag tttctctaat tttgaatcta tagttttgta
     5221 atatcttatc atggcaacac atcctttatt cagttttata taaatctcat catttaatag
     5281 tatctattaa ttaactaatt tatataactg aatatatgta ttagttttga ttttagatat
     5341 tattaagtga tgaaaatgta aaaaattctc tccgcaacgg actagtatcc attgcccatc
     5401 actctcctta tttattattt tatatatagc tatttatgaa atgcgtattt taagcactct
     5461 ttaatataat accttttcca ggtatataaa tcaagttttg atttgattaa ataatatttt
     5521 taccataatt tacacataaa gtatataagt tatatttaat ttgagtaaag aatatatata
     5581 ttctgagtga aattattaaa tatattaaaa aataagagtg tatatatata tttacttatt
     5641 tgcaataata tatttaaaaa agtattttat tttcaaaact agacttcatt tataatttat
     5701 catgaaaata aaaggaatat aaatataaga gaatgagagg ttattagaga tatgagtgat
     5761 tttaaagaaa ttttggcaga agagattaaa aataatccat ttggattgat aggtaaagat
     5821 tggacactta taacagcaga aaatgaaggt aaagtgaata caatgactgc tagctgggga
     5881 gggcttggag ttatgtgggg aaaagatgtt gcttttgtag taataagacc acagcgttat
     5941 actaaggaat ttatagataa tacagataaa ttttctctta cattttttga tgaagatttt
     6001 cgtaaagagc tttcatattg tggaaaagta tctggtagag aagaggataa gatatcacag
     6061 attggattta atatagaaca cttaaatgat acaccttatt ttaaggaagc taaaatggct
     6121 attatatgca aaaaaatgta tagtcagaaa cttgaacccc aatgttttat tgctgaaggt
     6181 atagatggaa gatggtatcc acaaaaagat tatcatactt tatatatagc tgaaataacc
     6241 aatgtacttg taaaagaaga ttagttataa gggttaatac tatctctaat taaattaaaa
     6301 tttcaataaa aggaaattga cacaaaaaag ctaaggacat aaatatgttt ataaagatta
     6361 ataaataacg tatttaaact ccttagcttg ataaaatttt aatttagttt tctttaaatt
     6421 ataattcaga aatcaattca ttgataacat cttcaacagt acttaattta tcaattttat
     6481 aggcatcatt tccacaaaat aaaagtgaat tttcaacatc tcctttaact gcattgatta
     6541 gagcttgaga aatacagtaa ggagtttctt ttggattaca aggaatcaaa caattatagc
     6601 acttctttat ttctggacga tttattttaa cttcttctat aaatttgttt cttatggcac
     6661 gtccaggaag acctacagga cttttaacta tatgaacatc ttcttcacta gcatttatgt
     6721 atgccatttt aaagttctca tgggcatcac attcgtaagt agcaacaaat cttgtaccaa
     6781 cttgtacacc acttgcaccc atatctatat attttttaac atcactacta gaagttatac
     6841 caccagcagc aactactggt atgtttctac caaatttttc tccataagta tttgcaacct
     6901 ttaaaatttc aacaaattcc ttatcatagt ctatagaatc tatgttatca agttcctcgt
     6961 tagagtatcc aaggtgacca ccagcttttg gaccttcaac aactattaag tcagcagtag
     7021 ttttctcttt tctatcccac atttttaaaa ttacattagc agcttttgca gttgaaacta
     7081 tgggtgctat ttttacatta cttcccttta ctaatgatgg tagcttatta ggtaatccag
     7141 ctccagatat tataaggtct acaccagctt taacagcttc ctttacgtat gtttcatatt
     7201 ctttcatagc taccataaaa ttaataccaa taataccacc atttgagatt tctttggcct
     7261 tagaaatatg tttttttata gctcttaaat ttgcatttat agtattagtt tcaaaatctt
     7321 cctcatcata tccaatttga actccagaaa ttacaccaac accaccaagc ttagcaactg
     7381 caccagctag tgatgaccta gatataccaa cgcccattcc accttgaatt ataggaactt
     7441 ttgcaactaa atctctaatt attaactctt ttatgataat ccctcctcaa tgtaaacatg
     7501 tttaaagaac tagattataa tacttaatat tattacctag tcctaattat tatactacaa
     7561 attataataa aagagtagtt atattttgta tagaagacga taattatatt atatttaaaa
     7621 cagctccatt agaatcgcaa cataactctt ttatttccca atcagatttt agtttttgta
     7681 ggtaattttt catattatcc acaaaactat catcattttc ttttattaaa gacattatag
     7741 taggtccagc tccacttaaa aatactccaa tactatttaa ttgttcagat ttttcaacaa
     7801 tatcattgta gttctcaatt agagttcctc tataatcttg atgcaatttg tcttgacaag
     7861 ctactttaag taggtcaaag tttttgttta gtaaggcaga tattagtaaa gcaactctac
     7921 taacattgaa taccccatca gaatatggta tttcctttgg taaaacacct ctagcttttt
     7981 ctgttgataa cttaaaatta ggtatcattg cacaaaattt aagttcttca ggaattttta
     8041 taatatcata atgtatattc tcattatcag taacagatac aatcatattt cctaaaatag
     8101 caggagcaac attatctggg tgaccttcta tttctgaagc tatatttagt aattgatttt
     8161 tatctaaatt agcacctgat agagcatttg cagctataac tccagctact atacaagagg
     8221 cactactgcc aagacctcta catataggta cttcactttg gatttttatt ttgataccag
     8281 caggtatttt ttctggttta actctatcaa aaaagtactt catagatgta taaactaagt
     8341 tgttttcgtt tttgtaagca tcttcacaac cttctatttc aagaccactt tcaatttctt
     8401 caacataaaa tttattgtat atatttaacg caattccaag acaatcaaat cctggaccta
     8461 tattagcact tgttgcagga actataatct ctaacataat aatcacctat acctttaaga
     8521 atttttttat agctagtttt aattcatctt tttcataaat atctttatgt agtatttctg
     8581 catttttaag atttttaata ggttttggaa tatctatttt agaaatactt gataatctat
     8641 caattatagc aaagtcatca aggtctttaa aatcacaatc tatagacttt agaacatctt
     8701 cagaaaactt gaatggactt gcagtactta caataacagt ttttgtgtta tcagaagttt
     8761 cttttttata tttttcataa caattgtatg caacagcagt atgagtatct atcaaataat
     8821 tataattttt aaacacattg tttatcgtgc ttttaacttc ttcttcattt gaatattctc
     8881 catagaatga ggctaaattt ttttccatat catcacttat tttgtagact cctttatcac
     8941 taaggtctga taaaagctta tttacaatat cagtatttct atttgatatt tcaaacagta
     9001 atcgttctaa attacttgaa attaatatat ccatagaagg agaagtagtt aatttcaatg
     9061 ttctattttt atcatataca ccagttttaa agaaatcata aagaacatta ttatcatttg
     9121 atgcacatat taatttgttt attggaagac ccatttgttt ggcataatat ccagctagaa
     9181 tattaccaaa attaccagtt gggacaacaa aatttatttt atcatttaaa tttatatcac
     9241 tattgctaac taatttcata tatgaataaa aatagtatgc aacttgtgga agaagtcttc
     9301 ctatattaat tgaatttgca gaagataata tataattatt atccaatagt ttttgattaa
     9361 attctttatc agaaaaaatc ttctttacac ctgattgtgc atcatcaaaa tttcctttta
     9421 taccaactac atatgtattt tttccagttt gagttttcat ttgaagtttt tgtactgggc
     9481 ttacaccatc ttctgggaag aatacaatta tctttatttt atctatatct ttaaatccct
     9541 caagtgcagc tttacctgta tctccagatg tagcagtaag aatcacaaca tctttctcta
     9601 aattatcttt ctttatagat gttttaagaa gatgaggcat tatagtaagt gccatatctt
     9661 taaatgctaa agttggaccg tgatatagtt ctaagaaata agtatcattt actttattta
     9721 atggagctat actactacaa tcaaactttt catcatatgc attttctata caatttttaa
     9781 tttcatcttc agtaaaatca cataaaaatt tactaagtac aaaaaatgct atctgagaat
     9841 acgttaaatt tactaaattt ataagttcat tttttacatt gggaaattca cttggaacat
     9901 aaagaccacc atcatttgat attcctttta ttattgcttg tgatgcagtt actttttctt
     9961 cggtgcctct cgtacttgaa taatacattt tttataatcc tccatatatt ttaaattttt
    10021 aattagctaa ttaaattaga tacagaatat tttaaaaact gaatttaggt aataaaaaaa
    10081 gaccctcatc cctaaaaaag ggacgagaat ctgtttcccg cggttccacc caaattgatt
    10141 ttaaaaaatc cacttgatac tttaacgcaa gtaataaaaa cgtctattcc tactaaaatc
    10201 attaagataa cttcggatag aagctcctag gtagttttca atccttctaa ttagggaaac
    10261 atttcagcta gtagtttcct tctctggtaa tcgattcaga tttactcttc ctaatcaatg
    10321 cctttcaata ttcttaatat tattaattat tctagtttat aaaacagaca aagtcaagta
    10381 ttaatataaa aatttggaaa aaatattttt taaattgtaa aaaaattgta atcaaattga
    10441 gtattagaaa aatatttttt taactttttt aaaagtttaa aaacctagat tttcatagtg
    10501 atgaagctgt ttttaatgaa agtaatcaaa agataactat taa
//
LOCUS       DACULQ010000133        10315 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.134, whole genome shotgun sequence.
ACCESSION   DACULQ010000133 DACULQ010000000
VERSION     DACULQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.134"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            187..1275
                     /locus_tag="JA154_13830"
     CDS             187..1275
                     /locus_tag="JA154_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996924.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLISKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            1303..2094
                     /locus_tag="JA154_13835"
     CDS             1303..2094
                     /locus_tag="JA154_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996925.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(2209..2772)
                     /locus_tag="JA154_13840"
     CDS             complement(2209..2772)
                     /locus_tag="JA154_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7996926.1"
                     /translation="MYLMNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNM
                     VVPSIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPN
                     LSGKKIEFILVTLDGHTHKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYF
                     NSTIPHVYENYDDEVCISVWAMTPPSF"
     gene            2959..4161
                     /locus_tag="JA154_13845"
     CDS             2959..4161
                     /locus_tag="JA154_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996927.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            4218..5198
                     /locus_tag="JA154_13850"
     CDS             4218..5198
                     /locus_tag="JA154_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAT7996928.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            5446..6399
                     /locus_tag="JA154_13855"
     CDS             5446..6399
                     /locus_tag="JA154_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996929.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            6389..7345
                     /locus_tag="JA154_13860"
     CDS             6389..7345
                     /locus_tag="JA154_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAT7996930.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            7342..8097
                     /locus_tag="JA154_13865"
     CDS             7342..8097
                     /locus_tag="JA154_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HAT7996931.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            8189..9154
                     /locus_tag="JA154_13870"
     CDS             8189..9154
                     /locus_tag="JA154_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAT7996932.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
     misc_binding    9253..9516
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9667..10218
                     /locus_tag="JA154_13875"
     CDS             9667..10218
                     /locus_tag="JA154_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HAT7996933.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT         4054 a         1046 c         1734 g         3481 t
ORIGIN      
        1 tatagataaa cataattttt ctaagtgtta tttatttgta gagctgaata aaagtgaaaa
       61 ttcttatcta gaatttattg atataattaa aaaaacaagt ataaaagaat taactatagt
      121 atgttgtagc taaagtgttt taacaggata aaatatatca cgattttaag ataggagttg
      181 agtcatatga gagattttag cacgtattct aaagatgatg ttattttttt attaaaagat
      241 atatcaaatt taattgaaga agaaggaaat aaagaaagag aattaaaaat acaaaatggt
      301 agacattatt cagaaatgat accaatagag tatgaagtta gtgaagatta tctgaattta
      361 tatcatgaga aactaagaga aaataaagat aaacttgcat tttctatagg agttatgtgt
      421 gaaaaaataa ttaaaaaaaa tggaaatgat gtagtactag tttcacttgc tagagcagga
      481 acacctattg gtattttggc aaaaagatat ataagaagca agtataatgt aagtttacct
      541 cactatacaa tctctataat aagagataag ggaatagata tgaatgcaat tgaatatata
      601 gtaaagaatc atccgagctc aaaaattcaa tttttagatg gatggactgg taaaggaact
      661 atatcaaaag aacttgaaaa agcatgtgat gaacttaatg ttaaatttaa aaaatatttt
      721 gactcaacac ttgctgtatt agctgaccct gctggatatt caggacttta tggaattaga
      781 gaagatttct taataccaag tgcatgttta aattcaacag tttctggttt agtaagtaga
      841 actgtactaa gagaggattt aattagtaaa aatgactttc atggagctaa gttttataga
      901 catttaaaag ataaagatga atctatgaac tatatcgaaa ctatagaaga atgttttaaa
      961 aatcaattta agaatatatc tgatgaagtt gaaaactggg aaagtgacat tataacaaga
     1021 gatggatact ttgatgtttt aaatataaaa gaaaagtata acataacaga tattaacttt
     1081 atcaaaccag gtgtagggga aacgactagg gttttactta gaagagttcc gtataagatt
     1141 cttgttaaag acttaaatga taaatcttta gaccatatat tcatattagc taaggagaaa
     1201 aatgttgaag ttgaacaaat ggatttaaaa gcctataaat gttgtggaat aataaaaaat
     1261 atgaaagata tttaggaaat atattagaca ggagaatatt gaatgattgt tttttcagat
     1321 ttagacagga gtatcattta ctccaataaa tttttaaatg ctgatagtaa atatgctaat
     1381 atagaaattt atagggaaaa agaaatatct tatatatctt tagatactat aaatttaata
     1441 aagcaaatac aatattatgg tatgtttata cctacaacta ctagaacagt ggagcaattt
     1501 aagcgtatag aatttaataa atatgggatt tattttcctt ggagtataac ttcaaatgga
     1561 ggagttatac ttaaagataa tgaaatattg aaatcatgga gtgaaaaaat agataaacta
     1621 aaatctgatt atgagcccat tgaaagtatg attcataaat ttaaagacta tttgaatgta
     1681 gatggaataa ctaattttaa agttgcagaa gatacatttt tttatatagt tgttgattta
     1741 tcaagattta atttggattc aatcaaagaa tatacaaata tattggaatc aaaaaattgg
     1801 aagttttatg tgagtggcag aaaaatatat tttataccaa aagaaatttc aaaagaaaat
     1861 gctataaaat acttgactaa agaattagga atagaatatt tttatgcagt tggagattct
     1921 attatggact atggtatgct taatatctca aataaatcct atgtattgaa gcatggtgat
     1981 ataaataaaa atgaaataga aaattcattt atatcaagtt ctttcaatgg aatgagtgga
     2041 actgaagaga tactatctaa cattttagac gagaactgcc tacttaatat ataatgactg
     2101 atatataaat aataaactag aaaaatctga aataaattat agtaaagttt atcatactaa
     2161 tttatttcag atttttatca atttatattt aagtaagaac tcaatatgtt aaaatgaagg
     2221 tggagtcatt gcccagactg agatacatac ctcatcatca taattttcat acacatgtgg
     2281 tatagtactg ttaaaataaa aactatctcc ttcctccaat agatattcct tatttccaag
     2341 tgttatcttc attttacctt ttattatata gccacattct tctccttcgt gtgtcacaaa
     2401 ttcactgttt ttgtgtgtat gaccatctag tgtaacaagt ataaattcta tttttttccc
     2461 agataaatta ggaacaagaa gttcgtaaaa ttttgtagaa tcattagtta ctattttttt
     2521 tcgattattt ttctttacta ctatatcaga gtcttctctt ccaatctctt caaaaaaata
     2581 acctatattt atatcaagag cttttgatat tttccacatt gctacaatag atggaacaac
     2641 catattacgc tcaatttgac ttattaagcc tgtacttaaa tctgaatttt ttgctaaagt
     2701 agagatactt atatttttag attttctaag agatctaatt ttgcttccaa catctatatt
     2761 cattagatac aaaccccctc agacatgatt ataacatata atttataaag attaataaaa
     2821 taaaaaaaaa gttttacaat acaagtattt tttatgctat attgaatttt ctgaaaagat
     2881 gcattataat agtattaata ttcaatatat tgaatattga taacgatatt ttaatttaat
     2941 gaaatggagg taattaagat gaagagtttt gtaagtcctg ataatatttg ggtattatgg
     3001 gcagttgtaa ctggatgggc agcatttagt atttatttgg aacaaaagta caattgggca
     3061 tcaaaagtgt cgggagcaat aatagctctt gtaggagcaa tgttattatc aaatctaaat
     3121 attataccag tagagtctgt tgtatatgac caagtatggg gatatgttgt tcctttagcc
     3181 atagctttat tgttatatca atgtaacatt aaaaagatat ggaaagaaag tggaagattg
     3241 ctcattatat ttcttgtagg ttctgtagga actgtacttg gagctatgat tggattttta
     3301 gcattaaaaa atgtagtacc agatttaaat attgttgcag ctatgatgac aggttcttat
     3361 ataggtggaa atgtaaactt tgctgctatg tcaggggcgt ttgatgcgcc aggagaatta
     3421 gtatcagcaa cagttgtagc agataatctt ttaatggcat tatatttctt tgtcttaata
     3481 gctataccat caattggatt ttttagaaaa cattttaaac atccacatgt agatgaaatg
     3541 gaaagtatag gtataactga aggagaaaca gtagcagcaa attattgggg aagaaaagaa
     3601 atatctttaa aagatatagc aatagccatt ggtagcgctt ttataattgt cgcagtatct
     3661 gtagaactat caacttggtt taaagctata ataccattat caaatccttt tttagcaatg
     3721 ttaaatactt tatttggaaa tcaatatcta ataataacta ctttgactat gttgtttgcc
     3781 acatttatgc ctaatttctt tggtgatata aaaggagcac aggaattggg tacattttta
     3841 atatatatat tctttgttgt agtaggtgtt cctgcttcaa taacattaat aattcaaaaa
     3901 tcaccattac tacttttata ctgcggaata atggtactaa taaacatgct agttacattt
     3961 atagttgcaa aaatatttaa gtttagttta gaagaagcta ttcttgcatc taatgcaaat
     4021 attggtggtc ctacaacagc agcagctatg gcaatatcaa aaggatggtc taagctagta
     4081 gggccaatac ttatagttgg aacatttgga tacatagtcg gtaattattt tggtctttta
     4141 gtaggaaata ttttgatata attttacgtg aaaaaaagtt gttttttaga aagattttta
     4201 tttaattgga ggttgttatg attttcgatg gacattcaga tacttgggca gatgtaacaa
     4261 taagaaggct taacggggaa aaagatgtat ttagaagaat ccatcttgat aattttaaaa
     4321 gagcaaatat agaaggagct atttttgtaa tatggataga cccaccatat gataaagagc
     4381 ctgaaaaaag agttaaacaa attataagct ctataaaaga tgaaattgaa gatatgaaag
     4441 atgttgtaaa catagtaaaa aaatattcag atatagataa aggtataagt gaaaacaaaa
     4501 taaatatttt aattggtctt gaaggattaa gccatattgg aagaaatata gatttactag
     4561 attattttta taaagaagtt ggtgcaagac atgcatcatt aacttggaat gaacagaatg
     4621 aacttgctac aggatgttta ggggatgaaa atcgtggttt aactgaaata ggtaaaaaag
     4681 cagtcaataa aatggaaaaa ttgggcatga tagtagatgt gtcacattta aatgaaaaat
     4741 cattttggga tttaatgtct gttgcaagta agccagttat agcttctcat tctaatgcta
     4801 ggagtttatg tgatgctaaa agaaatctta cagataatca attaaaagaa atttcaaata
     4861 caggtggatt agttggaata aatagtttta gaggatttac taatagtaat cctaagaagc
     4921 aagatttaat acatttggtt gaccatatag actatatggt aaatcttata ggtatagaac
     4981 atgtatcttt tggttttgat tttagtgatt acttagggaa agagactata ggttctttta
     5041 ctggaaatgc tagtgtgcca agtgtagatg gtcttgaaag cacacataca gcacagaaca
     5101 tagtagtaga gcttgaaaat agaggttata aaaagaaaga tatagagtta atctctagtg
     5161 gtaattttta taggctcttt aaagatatac taaaataata taataaagaa cttaaattta
     5221 taaaatagta tatttataca aataatatct acatatttaa attggcgttc ataatttatt
     5281 aaattaaatt ttaatgttac tagaagtttc aattctagta acatttttta tgttaaaaac
     5341 agatatagat atatattgac aatgataatt attattgata aactataaaa gtgaaaatga
     5401 taataatttt cattttaatt aataaaagat ttttaaggag ggagaatgaa gaaaaggtat
     5461 ttagtcatag gaataataat tttgtcttat ttttctttat ttataggagc tgaagatata
     5521 aatataatgc atatatttgc caaaaatcag cataaactta tgatatttat catgagtaga
     5581 ataccaagat taataagtat attgattgct ggtgtaggaa tgagcatagc aggtcttata
     5641 atgcaacaga taagtaagaa taaatttgta tcacctacaa caggtgctac aatagatgca
     5701 gcacaatttg ggatagtaat atgtatgctt ttagtaccaa ctgcctctat ttttacaaag
     5761 acaataatag catttgtatt ttctcttgta gggactttta cgtttatgaa aataataggg
     5821 aagttacaat ttaaaaatat aatctttgta ccattagtag gtataatgtt tggaaatata
     5881 ataggttcta tgacggattt tatcgcgtat aaatacgatt tgagtcaaaa tgttagttct
     5941 tggatgcaag gtgacttttc tatgatactt aaaggaaatt atgagatatt atatatcaca
     6001 atacccctta taatattagc atatatttat gcaaataaat ttactgttgt tggtatgggt
     6061 atggattttg cgacaaactt aggtcttagt tacaaaagaa ttgtcaatat aggtctgata
     6121 atagtagcac ttgttacagt atgtgtagtc gttacagctg gtaatatccc ttttataggt
     6181 ctaatagttc ccaatatcgt atccttgtat atgggtgata atataagagc aagcatatgg
     6241 tatactggtt tacttggagc gatatttgta cttatatgtg atatttttgg aagaattatt
     6301 atttatccat atgaaatatc tatagggctt acagttggag ttataggaag tatactattc
     6361 ctttatttaa tattaaggag gaatgtaaat gaagcttagt ctcaatcaaa aaatttactt
     6421 tgtaagtata ttactattca tatcaatagc actttttata atttatggaa tagacatgaa
     6481 tcatttagaa tatgcattat cacaaagaat accaaaaata tttgctatgg tattaggtgg
     6541 aggatgtata gcatttacta ctgtagtgtt tcaaacaatt acaaacaatc aaatattaac
     6601 accaagtgta ctaggtttag attcattgta tgtaatgatt caaactataa tagtattcat
     6661 atttggttca agcagtagtc tgattataaa tgaaaattat aattttatta ttaatgtagc
     6721 tatgatgatt ggagcttcat tgttattata taagacgtta tttgaaaaaa ataaaaataa
     6781 tattttcttt ttaatcctag taggaatgat atttggaacc ttatttaaaa gtgcaacaac
     6841 atttattcag gtaatgatag accctaatga atttttagct ttacaaacta gtataatggc
     6901 aagtttaaac aatataaaca ctaatgtatt gttaatagca tttataataa ttattgccat
     6961 aatacctttt atatatgatg aaataaagta tttagatgta ttgtcgttag gtaaagaaca
     7021 agcaattaat ttaggtgtag attttgataa agcagttaaa aaaatgataa tattaatagc
     7081 aattttagtc tcaatatcaa cagcacttat tggacctatg acatttttag gacttttact
     7141 tgctaatata acaagagaaa tttttaaaac atataagcat acatacttga tttcgggttc
     7201 tatgttgata gggatgatta cattgataat aggtcaattt tttatacaac atgtatttaa
     7261 gtttgataca actttaagtg tagtaataaa ttttatagga ggaatttact ttattcaatt
     7321 actattaaag ggggcaaata gatgatagag ataaaaaata tatttaaaag atataagaat
     7381 aaaaatgttg tagatgatgt ctcttttagt atagaaaagg gaaagataac atcttttata
     7441 ggaccaaatg gagctggaaa aagtacagta ctatcaatag ttacaagact tataggaggt
     7501 gatggtggag aagtaataat agaaggaaaa agcttgacta attatagcaa taaagaatta
     7561 gcaaaaaaaa tagctattct taaacaatct aacaatataa ctttaaagct tacaataaga
     7621 gaattagttg gatttggaag atttccatat agtgaaggta atctaactaa agaggatgaa
     7681 aattatattg atgaagcaat agaatatatg aagttgactg atatacaaca taagtattta
     7741 gatgaattga gtggaggtca aagacaaaga gcttatatag caatggtaat agctcaagat
     7801 acagagtaca tattacttga tgaaccactg aacaatttgg atatgaacca ttccgtacag
     7861 atgatgaagg tacttagaag tctttgtgat gagttagata agactatagt attagtaatg
     7921 catgatataa attttgcatc atgttattca gataatatag ttgcattaaa aaatggaaaa
     7981 gttaaaaaag taggtcgtac agatgagata gtaaatgaaa aagttttaga agatatttat
     8041 gagatgaatt ttaacattaa gaatataaat ggaaatcgaa tctgtattta tttttaaaat
     8101 attgtatatg aaaataaact tgctatatga ttttttataa agtttttaaa ttaaaataaa
     8161 gattaatagg agagaggaaa atattgatat gaataaaaaa gcagcaatag tagcagcagt
     8221 agctattata ggattagtga cagtgtttgc attaggtgga agtaaaaaga atgaatcaaa
     8281 aacatcagaa aattcaaata atactatgaa gataactcat aacttgggag aaacagatgt
     8341 aaaattaaat cctaaaaaag tagtggtatt tgattattcg gctttagata caatggatgc
     8401 attaggtgta gctgagaatt tagtaggact tccaaaagcg agtttaccag catccttaga
     8461 aaaatataag gatgacaagt atgcagattt aggaggatta aaagaaccag atttagaggg
     8521 tataaagtcg gcaaatccag atttaattat aataaatgga agacaagaag atttttatga
     8581 gcaattgtca aaaatagcac caacaataag tacaagtaaa gatgataaga agtacttaga
     8641 atctgttaaa aataatatag acaaaatagc aaaaatattt ggagtagaag aaaaagcaaa
     8701 tcaagaattt agtaaaattg aaaagaaaat agaaactttg aataaaaaag taacagataa
     8761 aaatttaaat gctttaacta taatggtaaa tgaaggtaat ttaagtgtat ttggtgaaga
     8821 atcaagattt agtatattat acaatagttt tggatttgaa aataaagata aaaatataaa
     8881 agaatcaagt catggacaaa atattacttt tgaatatata gctaaacaaa atccagaagt
     8941 tatgttcgtt atagatagag gaatagctac tggcagtgat gtaaaagaaa gctcaactgc
     9001 aaaatctgtt ttgaacaatg atataataaa atctatggat gcatataaga atgataatat
     9061 aatatattta gattcaccaa catggtatgt aaatgatggt ggtctaacat ctttaaataa
     9121 aatgatagat gatgcttcaa aagcggtaaa ttaataatcc gatataatca aaatatattt
     9181 tttataaaaa ggtattgaca ttttttagaa aaaagaatat tattatttta aaacaaaatt
     9241 caatacaata aattaaatcc tatgacaagg aagagtagtt aaatttaggt tcagtataga
     9301 gagctgagga tggtggaatc ttagcaagaa aaaatttgat gaatggccct tagaggaata
     9361 aattgaaatc atagttttga gagtagactt tatcgtcagt cgcacgttat agcgaataag
     9421 gtatgtaagt acctgataat gagatgtatg aaaattgaat tttatattaa tttttatata
     9481 atttaggtgg caacgcggat aatctccgtc ctattagttt aggacggagt tttttatttt
     9541 actgaaaatt taaaagatag aaggtggtga gaaagatgtt atgaatttca aaagctaaag
     9601 atgatgatat agtaggttgg caaaaaaata aattgaaaat taaaaaaata ggaggaagta
     9661 caaaaaatga aaacaaatac aaaaaaatta actttaaatg caatactttt agcgatggga
     9721 ttattaatac atcaaatcac accagctata ggattaccaa tgaagccaga tgtaccacta
     9781 gcaatgttat ttgtaatatt ggttttaaat agagatgatt ataaaacttg tttgatagcg
     9841 ggtatagtaa caggaatatt tacagcactt acatcaagtt ttccgggagg tcaaattcct
     9901 aatgtaatag ataaaactct tacagcaaat atagtatttt tattaatgaa tattagttat
     9961 aatatgcctt ttataaaaaa attagctaag aaaacacaag atactgtagt agttgcaata
    10021 ataatgccaa taggaactct tgtaagtgga actatctttt tattagcagc tcaagctata
    10081 gttggtcttc caggggcttc atttacagct ttattcttag cagtagtatt accagctgtt
    10141 ttaataaatc tagttgcagg aatattttta ttcaagattg ttagtctatc aataagaaga
    10201 gttagttatc aagcttaaac acgaaataat attttacttt ataaagcatt ataataacta
    10261 aataacataa gaataaagta gattataact attttattct tatgttgttt aaaga
//
LOCUS       DACULQ010000134        10162 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.135, whole genome shotgun sequence.
ACCESSION   DACULQ010000134 DACULQ010000000
VERSION     DACULQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10162
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.135"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..866
                     /locus_tag="JA154_13880"
     CDS             <1..866
                     /locus_tag="JA154_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996934.1"
                     /translation="YKYILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIEN
                     ENLSKLYSQNLYDKLRISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGH
                     SLSTTIIQLNAIEKLLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSL
                     FKIKELVKSFIKLTNINVKLTISKNTWNLSRNQSITLYRLIQESLSNSSRHGKATEIR
                     IFITFNTSNLIITISDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRAS
                     FPKFSGGDFFE"
     gene            859..1506
                     /locus_tag="JA154_13885"
     CDS             859..1506
                     /locus_tag="JA154_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7996935.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1635..2570
                     /locus_tag="JA154_13890"
     CDS             1635..2570
                     /locus_tag="JA154_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="export ABC transporter ATP-binding protein"
                     /protein_id="HAT7996936.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIIEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2584..3681
                     /locus_tag="JA154_13895"
     CDS             2584..3681
                     /locus_tag="JA154_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996937.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQNSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3684..4748
                     /locus_tag="JA154_13900"
     CDS             3684..4748
                     /locus_tag="JA154_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996938.1"
                     /translation="MIRLTIANIKRYLKNHNLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNELDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            4992..5489
                     /locus_tag="JA154_13905"
     CDS             4992..5489
                     /locus_tag="JA154_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HAT7996939.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5665..5913)
                     /locus_tag="JA154_13910"
     CDS             complement(5665..5913)
                     /locus_tag="JA154_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996940.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(5979..6995)
                     /locus_tag="JA154_13915"
     CDS             complement(5979..6995)
                     /locus_tag="JA154_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HAT7996941.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7053..7820)
                     /locus_tag="JA154_13920"
     CDS             complement(7053..7820)
                     /locus_tag="JA154_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAT7996942.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7888..10077)
                     /locus_tag="JA154_13925"
     CDS             complement(7888..10077)
                     /locus_tag="JA154_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HAT7996943.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3531 a         1562 c         1181 g         3888 t
ORIGIN      
        1 aatataagta catattgttc tcttttgtta taatatcttc tcttattatt agtttagata
       61 aaaatatata tttggcactg gagtcaagct ctatattgct aattataact gtactttcta
      121 tatatataga aaatgagaat ttatcaaaat tatacagtca aaatctttat gataagctta
      181 gaatttctaa agatgaatta aaaaaagtta atgctgattt ggaaacttat gcaagttcta
      241 tagaagaatt agcaatttta aaagaaagaa atcgaatatc aagagaaata catgatagtg
      301 taggacattc tctttctaca acaataatac aattaaatgc tatagaaaaa ctcttaaaag
      361 acaaacctct aatttatgac ttagtacatg aacttagaga atttgtaact gaaagttttc
      421 aagatgtaag aagggctata tctgagttaa aacctgttga atatgaaaat taccaaagtt
      481 tatttaaaat caaagaatta gttaaaagct ttattaagct tacaaatatt aatgttaaat
      541 taactatttc aaaaaatact tggaacttat ccagaaatca atctataact ttatatagac
      601 ttatacagga atccctttcc aactcatcta ggcatggaaa ggcaactgaa atcagaattt
      661 ttattacatt caatacttca aatcttataa ttactattag tgataatggt ataggatgtg
      721 gcaatataaa aaaaggaaat ggacttaata gtatcaatga gagaatatat gaattaaatg
      781 gtaaagttga atttgataat tctaataatg gtttcacaat tagagcttct tttcctaaat
      841 tttcaggggg tgattttttt gaataaaatt aaagttctta tagtagatga cgaaaaatta
      901 attagaaaag gtcttaaaat tatattatca tcttataatg atttagaaat tgtaggagat
      961 gcttctaatg gatatgaagc tttagaattt tgtaaaatga atgatgtaga catagttctt
     1021 atggatatac gaatgaaagt atgtgatggg gttctaggta caaggcttat aaaggaatat
     1081 aataattcta taacattatt aattttaaca acttttaatg atgatgaata tataaaagat
     1141 gctatgaagt ttggtgcttc aggatacctt ttaaaagata gctctgatga agtattgcat
     1201 gaagggattc gttcttcatt ttttggcaat attgtgcttg ataaaagtgt agctgaaaaa
     1261 atcatgacct ctgaaaaaac ggttaaacaa gagcatcttt atgacatgta caatttaaca
     1321 gaaaaagaaa tatctataat tagactaatt gcaaatggac ttaataataa agaaataagt
     1381 caagaactat tcttatctga aggtacaata aagaataata taactaatat tttagctaaa
     1441 ctagaacttc gtgacagaac tcaactagca atctttgctt tcaaaaataa aattgtaata
     1501 gaataaatct aaaaatataa agtgacttaa gtcacttttt ttagtgactt atagtaatag
     1561 aattataatt cacaattctt tattataata ttatagcttt ttaacttata caaaattatt
     1621 ttggagggat tactatggat atagtcagag taaataatat aacaaaaaga tttaatgata
     1681 aattagtact tgataacatc agcttttctg ttaaaaaagg tgagatattt ggacttattg
     1741 gcccaaatgg tgcaggtaaa tctacactta taaatataat tacaaatctt atgttaccaa
     1801 atagtggtag tattcaaatc aatggtttag atttatcaaa agattatata aaagcaaagt
     1861 ctataatagg gttagttcca caagaattag caattataga aactcttact ccttttgata
     1921 acttagagta ctttggagct ttctacggtt taaagggaaa actacttaaa gaaagaatca
     1981 ttgaagcatt agaagttaca ggtcttactg aagtcaaaaa gaaaaaggtc aaaaaattat
     2041 ctggtggtat gcaaaggaga ttaaatattg gaatagcact cttaaatcat cctaaaatat
     2101 taatattgga tgaaccaact gtaggtgtag acccccaatc aagaaatcat attttcaatt
     2161 ttataaaaga tatatctaaa aaacatgaaa ctactgtaat atatacttcc cattatatgg
     2221 aagaagttga acacctttgt tctaaaattt ttattatgga tgaaggtaaa gaaattgctt
     2281 ttggagataa tgattattta aaatcactcg tatctacaaa tacaaaatta attatagaaa
     2341 taaaaaatat aaatgcccaa cttatctttg atttaaaaaa tactaaaggt gttatatctg
     2401 ttttagaaaa taattcatta ttagaattgg atatagataa aaaattacaa cttacagata
     2461 tactctctgt tattgacaaa aacaattcaa aaataatgaa aatttcttat gaagaaccta
     2521 gcctagaaga cgtattttta aatctaacag gaaaaaattt aagggactaa tggaggtgat
     2581 tttatgaaat tatacatgag tgtaaaaact atgctaaaga gtttaaaaag ctcctttata
     2641 cttaatttaa tatatttttt agctttgcct ctgattttat cttggtttct tgggatggtt
     2701 acagaaagca tgtttcaaaa ccctataaaa acagaatcta cccccattgt tatatatgac
     2761 aaagataaca caagattatc aaatgatttg acaaagtatt tgaaaaatga tttatcttat
     2821 atattaacag ttaaaaaaga tgattctaag gcagaattaa agcttactat acctaaaggc
     2881 tatgagagta gtttattaaa tgaaaaatca aatactctaa acatagaaaa acttggaact
     2941 cgtgatgata ttgcaatatt gcttcaagat atattagata cttaccatga aaaattttat
     3001 ttaaataatt ctcagaaaat aagttcagaa gatttttcta aattatttaa taaaaattct
     3061 attgatactt ctataataaa taacaatgtt aaacagaact cttatgaata ctttgcttta
     3121 gtttcactag gatttcttgt aataattttt ataatgaaca atattttgtc aaattatata
     3181 agtgaatcaa agggactttc aaaaagactt tattctatgc ctataactag agtacaattc
     3241 ttaatttatg actttgtagg tttatggatt tattcattta tattcttatt attatatgtt
     3301 ttattcttta gaatacttgg aataacattt aaaggtaatt ttgccatatt gctcctactt
     3361 tgtgctttat catcttattt tatgacttca atatctacct ttgtaaccag cttttttagt
     3421 aaaaaatacg gtacagcaat agtttatgca ttactctttt tacagacaat ttttggaggt
     3481 atcttcagta tgataagtga tacatttact aagcttacaa gtttatctcc tacctattta
     3541 ataggagaat tgtttagtaa ttatgaaaca tttaaaacta tagattcaat aggagattta
     3601 attttcacat gtttaattat ctctacaata ttaattgcac ttgctattgt aaaggaaaaa
     3661 tataaatgga gggaggttta ataatgatta gattaactat tgcaaatata aaaagatatc
     3721 taaaaaatca taatctttta attaatatgg ttatgcttcc tattatacta attttttcac
     3781 ttaatttttt tatcaacaac tcaggaaatc aatcaatgta cttttctcca gtagcaattg
     3841 tatctgactc ttcaggaaag tatgaacata agttaataaa tagttctaaa ttaaaagaaa
     3901 attcatttag attaaatgaa ctagataagg ctatgaatct attaaaaaat aataaggttt
     3961 ctgctgtatt cgttttagat aaaaattttt caaatagtat agataaatta caacgacctg
     4021 tagttaaatg ctttaaaatt gaaaatggtg gtggtagtct ttgggcagaa tcacaaatag
     4081 aatcttttat tactaaatcg ttaaaattga aagtggataa aaatatagat gataaattga
     4141 caaagacaaa tataattgat aacaaaccag ataagaataa aggttcattt ttagtagtct
     4201 tcttgatatg ctattttatg tatattaatg cagcacatct tgcttctgat ttatttacat
     4261 taaaaaaatc aaatgtgcta aaaagactta tctctacaga taataaagat ataaaaataa
     4321 ttttcagtat atttttagga ctatttttta ttcaatcaat tgtatacact tttgctctat
     4381 tatgtttttc aattatagaa gactttaatt tgagtctgaa tgtattgatg attgtactag
     4441 caaatagttt tgtttccact ggatttgtat tttgggtagc tagagtattt aaaaatgaaa
     4501 gttctataag tcttatatct acattttatt ctttaatagg tctagctata tctatctcta
     4561 gtctaatacc atcaatggat aaattatcct ttatgactaa tctttcaaag ttcacaccat
     4621 tttattggac tatagatgct attaaaaaca atggtagtat tttaataaat ataatagctc
     4681 taatattaat tggaattata tttgtaactg caggaagtct taaactaaga gattttgcta
     4741 aaaactaata attattaaat ataattagta tctattttta tatagttgta aaagaatatt
     4801 tcaacatatt aaattatttt atcttatatt tttctcttta gtttcacaca tcaccaagtc
     4861 taaaccttat attatatatt aatatttttg acttgtcaag ttatttatgt tataatatta
     4921 atcattagat aattaaagca ttgtacctaa gttgcaatat ctaaaattca atttggaggt
     4981 ataactagca tatgtataaa attggtgaaa gtgtaatgta tccaaaagag ggagcctgct
     5041 ctgtaaatga tattgtaaca aagaaaataa atcatgagat gcaaaaatac tatgaattaa
     5101 gtgtcatttt taatagtaac cttaaaattt cgattccagt tctaaatgca gatagaattg
     5161 gcatacgtcc agttatggat ggaaatgatg ttgataattt tatccaatct attaataaaa
     5221 cagatggtgt atggattttt gatagaaaag aaagacttaa actgtaccaa gataaatttc
     5281 attcaggaga tgtttttgaa attgtaaaat taatcaaaat gcttatgata caagacagtt
     5341 caaaacaatt gtgtagcaca gataaggagt ttttaaataa agctcaaaaa tttgctttat
     5401 cagaattagc tgctgctcaa tgtaaatcct atacaatggt attagaggaa atgaaaaaac
     5461 atatattaaa ttcaaagaat acaaattaaa ccagtttttt aaactggttt tattttttat
     5521 actaatcatc catgctactt tagcttattt taaactgcct aatctaatat taataaagtt
     5581 atctcaatta gatagatgta ttttattcaa ataaaaaaaa ctaagagtaa atcatattac
     5641 tgatttactc ttagctaata aaatttaatc ttcttcaagt tcttttttct cttgtaataa
     5701 tacacttaat tcatttgctt ctaaaacttg tgcctctgca atatcaagta actcttgttg
     5761 ccattccaca catttatctt tgtaatcaaa aggtagcgtt ctgtcaacta tgtacttatc
     5821 aatacagtcc tccgcacttt taattatttc ttttctttca tcaataaatt tttctaattc
     5881 tactaaggct tcttcataag cttttctact catataaatt ccccctttgt acaattattc
     5941 tacattaaaa tgatttttcc tcttttttgt ataaaatttt atttgtatat aacttttcca
     6001 tcaatatctt ttatagtttg acatccagtt aatatcatag aactacttag ctctgattga
     6061 agtttattta cataagtttc tacaccatca gttgcaccac caaaagaagc agtcacaaaa
     6121 ggtcttccta ttaatactgc atctgcacct aaacctatca tttttagtac gtctaaacca
     6181 gttctcactc caccatctgc aagtattgta actttacctt taacagcatc agcaattttt
     6241 ggtaaaactt cacatgctcc tggtgtacag tcaagcactc ttcctccatg atttgataca
     6301 actattgcat ccacacctgc ttctactgcc attaaagcat cttcaacagt cattatacct
     6361 ttcaatataa atggtagttt agtagacttt actaactcct ttatttgttt aacattttta
     6421 ggaagtactg gttttccatg taatgataaa gttactagac cacaggcatc tatatcaact
     6481 ccaactgcaa atgcaccagc ttcttcagct aatcttatct tttttataat attttcatta
     6541 tcccaaggtt taataaatac aattccagct ccattatact ttttgactac atctaaattt
     6601 tccattaaaa aagcatctac tgccgtatca ccaaccattg cataaatacc actattagca
     6661 catcctgcaa ctactggttc tatgtaatct ctttcattaa tttttccacc catatttaaa
     6721 gtagttcctg ttactggagc tgcaaatata ggtgcactca ttttctttcc aaacatctct
     6781 atagatgtat caggatttga tacatcatgt attactctca tatttacttt tactttttct
     6841 aaactcttgc tattttctat aaaagaagaa ccacttcctt tacctcccat gccaggcact
     6901 tcacctgcac aagcaacccc attacaaacc ttacaaactt tacaacttcc atttaaattt
     6961 tctctagcac tttttaataa ttcattatat tccatcttta atcccccatt aactatagta
     7021 atttatatta tatacattat atcatattat atttacagta tatttttcaa aaaacttttt
     7081 ctgtggatag gagttatgcc atatttttca atagcttcat agtgttcttt agttccatat
     7141 cctttgtgag atttaaaccc atattctggg tatactctat catattggta cattatacta
     7201 tccctagtaa ctttagcaag tatacttgct gctgctattg atatagattt tgaatctcct
     7261 ttcacaattg gattttgaga tatgtcaata cctggtattg ttgctgcatc aactaataaa
     7321 taatccggtg ctttttttaa acaatttata gcctttttca ttgccatata tgtagcattt
     7381 aatatattaa attcatctat ttcttcattg tttactatac ctattccata atctagtgct
     7441 tcttctttta ttacttcaaa aagttcatct ctttttgcct cactcagttt cttagagtca
     7501 tttacacctt ctattttggt atctttttta aatactacaa cagaagctac aacaggtcca
     7561 gcaagtggtc ctcttcccgc ctcatcaatt cctccaatat ataaataacc tttatcatag
     7621 ccttcgttct caaatatatt tatagtttcc aatctttctt cttcttttct aatattatcc
     7681 agcttttttg ctagttttat agctagacct tgtacagatt ttctttcatc aactcttaac
     7741 agctctatat attccatata tttttctact tctaaagtct ctattatttc tttaatctcc
     7801 ctcacacttt tatcctgcat accattccct catctttcgt ttttaaaaat ttaattttta
     7861 aatatattaa aattttttaa ttagagttta attgcatcca ctatagccac attttaaaca
     7921 tatactacat ccacctgtat gtgcaattcc ttcacttcca cactcaggac acacaaattt
     7981 ttcatcttct tcaaaatttt tatttattat ttctttggct ttttcatctt tattatcttt
     8041 ttctttttta ttatttttat aatattcttt tttactattt tcttttttat cttctttatt
     8101 attttcttcc tttttttcat ttattaaaat tccactcctt ttacatccat ctctaaatat
     8161 tgttactcct ttaagaccag cctcccatgc cttaatatat atattgaaaa catcttcaac
     8221 tgtagtttca aaaggtatat ttattgtaga tgatatagaa gcatcaatat acttttgcca
     8281 tactttttgc atgtttattc tttcttcata tcctatattc atagcagtaa caaaaaaatc
     8341 tggtaaacat tcttcatctg taataccttt cttgtccata tactctttaa caattggagt
     8401 atatacttta taatacacat cctcatcatg caagctttca gtttttctta tatatgaaag
     8461 attaaatata ggttctattc caccactaat acctatcatt gtagatatag aacctgtagg
     8521 aggtatggtt aaaagttgtg aatttctaag cccatacttt ttaaccattt ccttagtgtc
     8581 ttcatttaga ttttctataa aaaacttaga tttagaaata ttttcatatt tatactcact
     8641 aaatgttcca tattctttag caattagtgc tgattgcttc acagcttcat ttaacatagt
     8701 cttaccaatt aaatcacata gttctaaaga ttcatcagaa ccatatctta ttcccatttt
     8761 tataagcata tcagctatac ccattacacc taaacctatc tgtctatact tatgtacact
     8821 atctctttgt tcttgtaatg gatgcaagtt caatccctca tctaagacat catttaaagc
     8881 tactacagct tctctaacgc aagattttaa tttattaata tcaaaagtag catgttcacc
     8941 aaatggttgt attataaact ctgacaagtt tattgagcca agtagacaac ttcctccact
     9001 tggaagtggt tcttctgcac aaggatttac tcctgaaaag caaaagttct tatcttcact
     9061 cataaaatta tatgttttta ttttatccca aaacaatata cctggttctg cataatccca
     9121 gttatttttc acaaatttat taaataaatc ataagcattt actatcttaa ctattttttc
     9181 tccagtagtt tcaacttcaa aagtacaatt atataacttt ttttctctta cagcttgcat
     9241 aaattcatct gttactctta cagaaatatt tgctttagta accttctcca aatctgtttt
     9301 tatatctata aagtcttcta tctctggatg atttacatcc atagaaatca taagagctcc
     9361 tcttctccct ctttgaccaa ttaagccagt tgtcatactg taaagttcca taaaagagac
     9421 tgctccagtc gtatgttttg cagcattgtt aactttcgct ccttttggtc ttagctttga
     9481 aatatcaatt ccaactcctc caccataact aaaagttcta gcaagttttt ttgcagtatc
     9541 aaatatatct tctatgctat cttttggtgg ttctattaca taacaattag aatatgttac
     9601 tttttttcct tctttctgta atcctctatt tgctaaaatt cttccagcaa atagaaatct
     9661 tttttgtctt attagttttg atattcttga actattattt gaaattctct ttatccattc
     9721 atcaaaactc tcaccattat gtctatactt gttcttccat attgattttt gtaattcatc
     9781 aaactcccaa gctttagtcc ttatttcaga gcgtctttct ctatataata tgtagtgttt
     9841 tgctatatct tttctatttg attccataag tagtacttca actatatctt gtatttcttc
     9901 tacggataag catttttcca tagaattata gtcattagct atttttcttg atatatcata
     9961 tgccaactct ctatctactc caccttcact atttatacta gctttataga cagcatcttc
    10021 aatcttactt ttactaaaaa gttcttttgt accatctctt tttattatgt atttcatttt
    10081 taaacctcca ataaattagt attaaattaa tacacacatt aactataact ctaaacaaac
    10141 tttttatata tttgtatatt tt
//
LOCUS       DACULQ010000135        10067 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.136, whole genome shotgun sequence.
ACCESSION   DACULQ010000135 DACULQ010000000
VERSION     DACULQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10067
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.136"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..613
                     /locus_tag="JA154_13930"
     CDS             <1..613
                     /locus_tag="JA154_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAT7996944.1"
                     /translation="QKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNEINEEELKPI
                     LDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKSYNLVNDSVL
                     AQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENAMYLAKKRYE
                     RVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            766..2769
                     /locus_tag="JA154_13935"
     CDS             766..2769
                     /locus_tag="JA154_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan-branching enzyme"
                     /protein_id="HAT7996945.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYENMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITYPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDSSI"
     gene            complement(2896..3207)
                     /locus_tag="JA154_13940"
     CDS             complement(2896..3207)
                     /locus_tag="JA154_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996946.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(3285..4103)
                     /locus_tag="JA154_13945"
     CDS             complement(3285..4103)
                     /locus_tag="JA154_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7996947.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSQLFAKKLEESLNPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            complement(4203..4727)
                     /locus_tag="JA154_13950"
     CDS             complement(4203..4727)
                     /locus_tag="JA154_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAT7996948.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            complement(4746..5888)
                     /locus_tag="JA154_13955"
     CDS             complement(4746..5888)
                     /locus_tag="JA154_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HAT7996949.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKKYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            complement(6168..8555)
                     /locus_tag="JA154_13960"
     CDS             complement(6168..8555)
                     /locus_tag="JA154_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7996950.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAIYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            complement(8548..9231)
                     /locus_tag="JA154_13965"
     CDS             complement(8548..9231)
                     /locus_tag="JA154_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7996951.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            complement(9348..>10067)
                     /locus_tag="JA154_13970"
     CDS             complement(9348..>10067)
                     /locus_tag="JA154_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7996952.1"
                     /translation="NRSLKIKFIKLEKEKESVKSLVTDISHQLKTPLASLKLYNTLLI
                     EEELEEDEANEFLLTNKASINKLENLINSLVNISRLEISMINIKKEDNDIKNTILNAV
                     KSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESIFNVLDNAVKYTQVNGEINISVEE
                     TANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADEIESVEGSGVGLYLSRKILEEQGG
                     NIIVSSKVGIGSKFSLFLTTM"
BASE COUNT         3430 a         1597 c         1157 g         3883 t
ORIGIN      
        1 acaaaaaaga aatgatgata gagttaatat ttatgtagat gataaatttt ttattgctat
       61 atttaaagaa cttgtttata catttaattt aaaaaagggg aatgaaataa atgaagaaga
      121 attaaaacct attttagatg atgaaatgta tataaaagct aaaaataaag cattaaatat
      181 tttatctcat gctgaccaat ctgaaaaaaa attaaaagaa aaactatcat cagaatttga
      241 tgaaaatata attgagaggg tcatagattt tcttaaaagt tataatctcg taaatgacag
      301 tgtattagct caaaaaattg ttaacacaaa tgttaaccta aataaatgtg gcaaaaacag
      361 aattaagcag aatttatata ataaaggtat caatagaagt acaatcaatg aagcagtatc
      421 tgaattagac aaggacactg aatttgaaaa tgctatgtat ctagcaaaaa aaaggtatga
      481 aagagtaaag aaagaagata aaaagaaaat atatcaaaaa atttctcaac atctttccta
      541 taaaggcttt gattatgata ttattaaaag agttttaaac aaacttttaa attttgatga
      601 atacgatata taagtataaa tactagggag gtaaattatg aaattaatca ttggtggctt
      661 aagcttttta ttaggattat ataatatata tcaattatat agagaagata gtatgagata
      721 aatattattt tttagatgta atatttatta tggaggagaa atacaatgaa aagtcaaaaa
      781 agatttaaaa ttttagatat tgatgtatac ctacaaccat ttgaatctga tataaaaaag
      841 cgaatgaaac attatgagaa tatgaaatca aaaacattga aaaactataa agattttagt
      901 tcttttgcaa atgctcatct ttactatgga tttcatcaga caaatgatgg attcttctat
      961 agggaatggg ctccaaatgc agatagtttg tcactaattg gtgactttaa taattggaat
     1021 agaaaatctc atcctctaag aaaaattggg tctggtaatt gggaaatatt tattcctggt
     1081 caaaatagct tacctcacaa atcagaggta aaggtacaag ttactgcaaa tggaaaaaca
     1141 tttgaccgta taccattata tataaaaaga gtaattcaaa aagattttgg tggatttaat
     1201 ggacaaatat ggcaaccaaa aactccattt atctggactg acaatgattt tgacttaaaa
     1261 aacataactt atccactaat ctatgaatgt catataggta tgtctactga aagcgaatct
     1321 attggaactt ataatgaatt tactgaaaaa atacttccta agataaaaaa agctggatac
     1381 aataccattc agcttatggc tattatggaa catccatatt atgcatcttt tggataccaa
     1441 gtttcaaact tttatgcaat ctcatctagg tttggtactc ctgaagactt aaaaaacctc
     1501 ataaacactg ctcattcaat ggggattgct gttttattgg atttagttca ttctcatgct
     1561 gtaaagaata ctttagaagg aataaatgag tttgatggaa gtgaacatca atttttccac
     1621 tcaggttcaa agggaaatca tcctgcttgg ggtacaaaac tttttaacta tggtaaacct
     1681 gaagtaatac attttttgct ttcaaatata aaattttggt taaatgagta ccattttgat
     1741 ggctttaggt ttgatggtgt gacttcaatg ttatatcata accatggtct tggagtttca
     1801 tttgatagtt atgaaaaata ttttagtatg aatacagata ttgaagctat aacatatctc
     1861 caatttgcaa atgaattaat aaaagaaatt aaacctaatt cgataagtat tgcagaagat
     1921 atgagtggaa tgcctggtat gtgtatccct ataaaagacg gcggaattgg atttgactat
     1981 agactcgcaa tgggagtacc tgatttttgg ataaaaacta tctctaactt atctgatgaa
     2041 gactgggatt tgggaaaaat gtggtatgaa cttactacta gacgacctgg agaaaaaaat
     2101 ataggatatt gtgaatccca tgaccaagca ttagttggcg ataaaacaat tattttttgg
     2161 ctagctgaca aggaaatgta ctggaatatg gaaataaact caaataacca tgttatcaat
     2221 agagcaatat ctcttattaa attaattaaa ttaataactt tttctctagc aggtgaagga
     2281 tatctaaatt ttatgggaaa tgaatttgga catcctgagt ggattgattt tccacgagaa
     2341 ggcaacaatt ggagctataa gtatgccaga agacaatgga gcctttcaga agataacaac
     2401 ttaaagtata aacaattatt aaattttgat aaatctatgc tcgaacttac aaaatacagt
     2461 gatattttta tccaaaatag tcatgaactt atacatatgg ataatgataa aaagattttg
     2521 gtgtacagta agggcaaata tttatttata tttaatttcc accctaatct aatacaatca
     2581 gttgatattt caaagtttca aaatacaaac tacaaaacga tattaaatac tgatatggta
     2641 gagtttggag gaaacacaaa aaaagaaaac ttaagagatt atgatgtata ctttagctca
     2701 actcctcctt cttctaaaat gcctattgcc tctcgtacag caatagtgct tttagatagt
     2761 agtatttaag gttggttttt ctagtatata acactcatat taaaataaac actaaaagcc
     2821 aaataacaat ttaaatacaa aatgttattt ggctaattta gtttgatact atttaactat
     2881 cttttacatt aagttctata atttccaact tttaatttgt ggtattatat tcatcttaac
     2941 agtttgattc tttaaagctt ttctaaattt ctcagttgaa gaatctttaa acattttact
     3001 agaagcactc ttgaggtctt ctatagattc ccaatgttgt atcataatcc acttttgaga
     3061 cttttcatca tataaaagct cagtatcaat aaaaccagat tgaatagaat gaaattttaa
     3121 ttctaagtca tttacaattt ctataaactc ttcttttgat aaattttctt ccacattaaa
     3181 ctcaactatt tctgtaataa ttttcatatg taaaacctcc aatttatttt cctatatcat
     3241 atcaaaaaat aaattggagg tattgtaaaa aatcgacagt ataattataa atagctgtac
     3301 tttatatttt ctttaatcgt cttattctca ccaataaatt taattggtga cgtccctgaa
     3361 aattttttaa attcttttat aaagtgtgct tggtcatagt actcattttc ataaataaaa
     3421 tcaaaaagtt catcatcttt ataattataa ataagttgat tgcttgccat tttaaatctg
     3481 taaattctct ttaaaatctt aggtgtatac cctgtatatt ttaaacagat tctttccaat
     3541 gttttaatat ttatatttgt atcatcacaa aatgctttta tattgctata tctattaaca
     3601 tatataaatg aatttagaag ctttacatat ttatttgaaa ttaaatcttc ttctaatatg
     3661 gataataata cttcttctaa atataaaaca attttttcta ctgattgtgt tggatttaat
     3721 gactcttcca atttttttgc aaatagctgt gatactgctt ctaggtcaac aatttgtctg
     3781 ttaaactctg aaattggtat ctttaaaaat ggatagagac caaatggata aaaagaaatc
     3841 cctataacac gtatatttcc atgttgaata ataaatccat gtttatccct tattccatta
     3901 aaatgcatat tactagcagt atagtcttga ttttcaattt tatattccat aacatcagaa
     3961 agattgagaa taatatctat actgtctgtc ggaagtaact tattatttaa cagtatattt
     4021 gattttgtct caaaatacca aataaacttt acccacggct ttaaacgaac atttttgatt
     4081 gaatatcttg taactgataa cattgtgaac taacctctat actaaaattt tttaattatg
     4141 tactccatcc taatattata cagtataatt tatgctatca taaattttag ttcactttat
     4201 atttatttta tacatttttt caatacctta gaaataggaa gcgctattat aagacctatc
     4261 aatacttgca ttaaattacc tggtattgaa gtaagaggta ctataaaatt accataaagt
     4321 ataacttctg ttacataata tccaaatagc atccaaattg atgaaacaca tattcctatt
     4381 acattgatta aaaaattatt cccattatta ccatttgccc atgcaatctt tccaagtaaa
     4441 taacccataa ctcctctaac tataaaggta aacggtgccc ataaagtcca cccagatatc
     4501 aaatcaaaaa gtgacatacc aatagcccct gataaagcac cttttttacc tccaaatact
     4561 attgctgata tgaacaacat tgcagttcct aaatgcacaa gcccaccatt tatagatatt
     4621 ggtaatctta tgtttataaa tttagttgca ataaatacta atgctattaa tagtgaggtt
     4681 tccaccatgt ctttcgttgt aaatttttgc ttagctgtaa cttccatact atatccccct
     4741 atctattaaa aataatactt attaaataaa tttaacaaat ttccatttcc tttgaatcct
     4801 aatgcactta aacttttatc caaaacattt agtacatgtt ctattttatt tagatttgca
     4861 ttctctccca tatgacctat tcttaaaata gtatccatat attgatttag tgatgttgca
     4921 acaagtgtat tataattttt aagcatataa tcagtaagtt ctaaagctcc tatactttca
     4981 ggaattttta tagctgtgac tgtattagaa tatccttctt ctaaaaatag ttctagacca
     5041 tattcctcaa tagattttct aacactataa gctatctttt catgtctatt taatacattt
     5101 tctattcctt cttctaatat attatcaagt gctttatcaa gccccattat gtcacttatt
     5161 ggcattgtat aagggaagta ttttttctca taatagcctt cccatatatt taaattacaa
     5221 taaaaaccta ttacatcagt ttttctattt tttatacaat tttttgcatc ttgacttatt
     5281 ccgactatag taagtcctgc tggtgcagaa atagcttttt gtgaaccacc taatattata
     5341 tctattttag attcatctac acttaatctt tctcctacca tacctgctac agaatcaact
     5401 acagttaata ttccatattt tttaagtaat ggacatattt tgcttacatc atttaatacg
     5461 ccagttggag tatcacaatg cactactgtt gcatatttaa aattactatc ctcatctaaa
     5521 aatgctttca attcatcaat atctatactt ttagtatact cacttgaaaa aagcacatac
     5581 tccccaccat acattgttac aaaatcttta aaaccttctc catatatacc attgtcaatt
     5641 acaagtactc tatctccttt ttctgtaagt gatgcacatg ctgcttcaag acctaatatt
     5701 ccttcaccac tcaatatgta aacatcgttt tttgttccaa ttatattacc aaacttctcg
     5761 catgtctttt tataaaattc aacaaaatca atatctatat ctggatttgt tgttttttct
     5821 gctcttgcta atcttacatt ttccctagtt tcagttggtc ctggtgcata attaatcttc
     5881 ttattcatat atatcagctc ctatttattt tctatacgat tattatacaa aattttcatt
     5941 ttatatggta caattttata cttttttaga attgtaccgg tacattttct aacatgaata
     6001 atatcagtat attagatata ttattgatta cataataaaa tacagagcaa acatctgttt
     6061 aaattgtttg ctctgtacta tgctttaacc tatttaatta tgaatctctt taactatatt
     6121 tttttatatg ttttttgcta gttaaactaa ccataaatat ttgcatttta ttcactgtat
     6181 cttactctat caataagaga ttctttatta aaacttcgtc ctatcaaata agttgttata
     6241 aattgagcta taagaataca cacaatcatc aacaagattg gtactattgg caaagaatat
     6301 gttgcataag ataatccagt cttttttaga aatataacag caatataccc taatatacct
     6361 ccaaatagaa tacttcctat catcattcca gaagtgtaat atattgcctc tgaattaagc
     6421 attttcacta attgtttatt agtaagacca atagcttgaa gcataccaag ttcttttttt
     6481 ctagtaacta tgctagttat cattgtgttg acaagattca taaatccaat cacacctatt
     6541 atgcctgtta gcgtgtatcc cattatcttt ataaatgaaa tagtagcctt atttgactca
     6601 attctactat ctataaaatc agtctctaaa aatccattat ttttagcaat cttcttgata
     6661 ctatctttga tttcttgata ctttccttct tctgtatata ttcccaagct actagttgaa
     6721 tcgtatttca aagttctatc tataaaatct ttccctatac caaaacttgg aacccccata
     6781 gctattgctt gaactttaaa ttcttttttt atctttttat ctccatcata taaagttaat
     6841 cttattttat ctcctgcttt tactcccatt tcttttgctg tatcaacatg agtaattact
     6901 atttcatctc cagtttgaag tctttcaaga tttatttttc catctattat attcttgttt
     6961 aatgatttta catcattttc atttatactt tgtaaaaggt catacttaaa ttcaacatca
     7021 tagtcctcca taccaatttt tacacttctt gcagattcta ttcttttaat tccttccatg
     7081 ttctttattt gattaaaaaa atctttactt agaggattat ccatttgtaa aatattcaaa
     7141 ttatctttag gatttttatc actgttcatt tcataaccag atagtctaac ttctatatca
     7201 taaggaaagt gttctcttgc cattttctca gcatcaaaac tttctaaggc agttgatact
     7261 gttataaata tagttccact aagtataagt gatgccaatg tcatgtaagt gcgtttttta
     7321 tttctttcta aattggcatg tgataatctc tttagattta tatttttata tccttttctc
     7381 ttactcttat tacttatttt atttcctgta tatcttactg cgtcaacaat ggatactttt
     7441 gatgctactt tcattggttt taaaagtgat aaaacaacag atataaagct tattaccaca
     7501 accgataata taactggtaa tcgtgatgat tttacatcta tatccataaa tgattttatt
     7561 ataatatttg caagtacata gcctaaaatt attcctattg gtatagctat tccggctaat
     7621 atgaaccctt ctttaaacac tatatttttt atttgtttct tagttgcacc aattgctcta
     7681 agttttccaa attcttgaac ctttgtaact attgaaatat aaaatatatt gtatattact
     7741 aaaatactag ataatacaat tactaaccct acaaaaactc cacccataat cgtttccata
     7801 tctggtttta aagcatttat ataattttca tttacagcag tattaatagt atccagtcct
     7861 atatcattag caattttatc taatttttct tttgcttgct ctattgataa attactttta
     7921 tcattaaatt tcacataaat attaaaatct tcttgtgaca tatctctagt atttctcata
     7981 tatgattctg atataatagc ataataatat ttacctgctt cagatatttc gctagttttt
     8041 aagattccac ttataatgaa ctctttttca atttttttat catttttata gtcatcataa
     8101 gatattttta tcttttcacc taaatcttta tttttataac caagtgcctt taaagctcca
     8161 ctatctaaca caatttcatt ctcttttgat ggcaattttc ctttgataaa ttcaaaacta
     8221 tttaatttca aagcatcttc attcatgtat gctagagaaa tatttgaatc atcttccagt
     8281 ttttcaacac ctactccatt catttcacca gttaattcaa catctgcatg attactcaat
     8341 atatcagcct gtttttcatc tacatttgct aatatacctt gataattgcc tgaatacttt
     8401 atagtatttt ctttttgata tgctcctgca tcaacaatat aaattccaat agaagaaagc
     8461 attaaggttg aaagtattat agtaattatt attagaatac tttttgattt attttgcttt
     8521 aaattacttt ttgctatttt agtagtatta atcattttta tcaccaacct ttgacaccct
     8581 gccatcttct atgaatataa ctctatcagc catttgggct atactgtcat catgagttat
     8641 cataactaga gtttgactgt acttcttaga catagatttt aatatactca taacctcatc
     8701 agacgtttta gaatctaggt ttccagttgg ttcatcagca agtattatag ctggtcttgt
     8761 agctaatgct cttgctattg ccaccctttg ttgttgacca cctgataatg tatttggtaa
     8821 aacgtcgtgt ttattttcaa gtcctaatga ttttaaaagt tcttttataa aagattcatc
     8881 tacttctcta ccatctaaac cgattggcaa cactacattt tcccatacat taagagatgg
     8941 aattaaatta tagctttgaa atataaaccc tatttttctt cttctaaata cagcaagttc
     9001 ttcttctttt aatgtaaata tatttttatt atctatatac accttcccag atgtaggtat
     9061 atcaagacct ccaatcatat gcaaaagtgt actttttcca gaacctgatt ttcctattat
     9121 tgcaacaaat tcaccttcat ttatttctaa gtcaacattg tccaaagctc ttactaagct
     9181 ttctcctttt ccataatgct ttgataaatt ttcagtatat agtattttca taaaattatc
     9241 ctctcattct aagttatatt actccatatt ttattaattt tttatttctg gagctgttta
     9301 tacttatata ataaatgtag aggcttacat tttcctttca tttaatatta catagttgta
     9361 agaaacaaag aaaacttgct ccctattcct acttttgaag aaactataat atttccacct
     9421 tgttcttcta aaatctttct acttaaatac aatcctaccc cactaccttc tacagattca
     9481 atttcatcag cagaacctct ataaaaacgt ttaaatatat tattaaactc atttttttca
     9541 atccctatgc cattatcctc tattataatt ttaaagaaat tagcagtttc ttctacagat
     9601 atatttattt ctccatttac ctgtgtatac tttacagcat tgtctaatac attaaaaata
     9661 ctctcctcag tccacttttt atcatgtggt ataattctac tgttaaattc atttaaattt
     9721 aatgttatac ttttgctctt agccttaggg gtaacacttt ttactgcatt tagaatagta
     9781 ttttttatat cattatcttc tttttttatg tttatcatgc taatttctaa ccttgatatg
     9841 ttcactaaag aatttattaa attttctaat ttatttatag aagccttatt ggttaataag
     9901 aactcgttgg cttcatcttc ttctaattct tcttctataa gtaatgtatt gtataatttt
     9961 aaggatgcta aaggagtttt taattgatgg gaaatatctg ttactaatga ttttacactt
    10021 tctttttctt tttctaattt aataaacttt atctttaaac ttctatt
//
LOCUS       DACULQ010000136         9894 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.137, whole genome shotgun sequence.
ACCESSION   DACULQ010000136 DACULQ010000000
VERSION     DACULQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.137"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            116..1558
                     /locus_tag="JA154_13975"
     CDS             116..1558
                     /locus_tag="JA154_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HAT7996953.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1600..1953
                     /locus_tag="JA154_13980"
     CDS             1600..1953
                     /locus_tag="JA154_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996954.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2144..2965
                     /locus_tag="JA154_13985"
     CDS             2144..2965
                     /locus_tag="JA154_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAT7996955.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            3223..5106
                     /locus_tag="JA154_13990"
     CDS             3223..5106
                     /locus_tag="JA154_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAT7996956.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            5331..6011
                     /locus_tag="JA154_13995"
     CDS             5331..6011
                     /locus_tag="JA154_13995"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088702.1"
                     /note="catalyzes the interconversion of beta-D-glucose
                     6-phosphate to beta-D-glucose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAT7996957.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            6008..8293
                     /locus_tag="JA154_14000"
     CDS             6008..8293
                     /locus_tag="JA154_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAT7996958.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIKIIVYGKKYILEKCIKIDK"
     gene            8518..9411
                     /locus_tag="JA154_14005"
     CDS             8518..9411
                     /locus_tag="JA154_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase"
                     /protein_id="HAT7996959.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYSNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
BASE COUNT         4033 a          917 c         1753 g         3191 t
ORIGIN      
        1 tatgatataa taacaaatat ataattatta aattataata aatgactata gcgtagatgt
       61 aatactatgt tatagtcatt tttgaattaa acttgatata tcaaggggga agaatatgag
      121 aacaattaga agagttttag cattaggttt aacattagct atatttctaa taaatgtacc
      181 taatgtagat gcattaacat cagatacaat taaaggtaac aatatatatg agacggctgg
      241 attaatagca gataaaaaga gttatgatac agcaattatg gtaaatatgg ataattctat
      301 agcagatggt ctttcagcaa gtggtcttgc aggtgctgtt gatgctccaa ttttgcttgc
      361 acaaaaaaat aagataccaa atgaaacaaa acaaagatta aaaaatgtta aaaagatata
      421 cataataggt aaagaattat caataagtaa gtcagtagaa actgaactaa aaaacacagg
      481 ggcacaggta actagattgg gtggagatga cagaataaag actagttata gtgttgctaa
      541 agaggtaaat ggtatcaaaa aagttgatga agtgatatta actaatgcat ataaaggaga
      601 agcagataca ataagtgctg cacctgtatc agtaagagat atagctccta tagtacttac
      661 agatggaaag agtgtacctt tttcaacaag caatgtaaaa acatatgcag taggtgggag
      721 tatatcaatg agcactagtt tagttaataa aactaatgct aaaagacttg gtggttcaga
      781 tagatatgat acaaataaga aagttataaa agagttttat ccagatgcat cagaatttta
      841 tttgagtgat ggatatgatt tagtaaatgc acttacaggt tctacaattg ctaaggaaaa
      901 tccaattgta ttggtgtcag aaagtagtga taagtctata ttagcaggag cagataagat
      961 tactagatta ggttcaataa gtgacagtgt gtataataag tgtgtttctg ctgcacaaaa
     1021 taatggcgat tcgtctacaa aaggtgagtc acctatgaaa aatgagacaa gcatattagg
     1081 tcagccaaca gctagtttag aagcgtgctt aaaatgggca aaatctaaaa aagctaatga
     1141 tttatttata gagttaatac caatattata tgatactgct gttcaggaag gtgttaatcc
     1201 tgttttggca gtagctcaat ctgcaaaaga aactggtttt tgtaattttg gtggagtatt
     1261 agatgcatca tttaagaatc cttgtggact taaaacttct gtaggaggtt ctgatactga
     1321 taagaatgcc cattcaagat ttgatacttg ggaagaagga atattagctc aaattcaaca
     1381 tttatgctta tatgcaggac aagatggtta cccactttca aatccagtag accctagaca
     1441 tgaaaaatct ttatttggta aggctaaaac agttgaaagt ttatctaata attgggctgg
     1501 aggtcaatat ggacaagatt tagtaagaat gatgggagaa attgaggcaa caaaataatt
     1561 gaagattatc aaatatccaa taaataagga ttgattatta tgaaaaagag taatattttt
     1621 agtatcatat ttcctacagt cataatggtg ctaatgtttt taacttttgc tacagatata
     1681 tttaatattc cagatattca atctaaaagt atttttgttg tgggaatggt tttgattttt
     1741 ccaatttctt ttttgataca agggataatt tgtgtattaa ataagactaa ttggatttta
     1801 tctcttattg tgtctttagt aacttatata gtattaatgt atatattttt aaatgattct
     1861 gcatatatat atgttttact ttatacagct ttttatatga ttggacatat aacagctaaa
     1921 ctctattaca aaattaagac ttttaaaaat taaattgaag tatataaaag taacattagg
     1981 agaagtatcc ttaatgattt tctaaaatca gaaataggtg cttctctttt ttgtaaaaat
     2041 aaaagtatgt aaataaaatt taaccattat aattttcttt taacaaatgt atacaataaa
     2101 cttgttgtat aattaagata gataaaataa agggagggat aatatgaatt taatagataa
     2161 tcatattcat actaattttt caagtgatgg aaaggattcc atggaagata caataaaaaa
     2221 agctataagt attggtgtta gatatttgac ttttacagac catttagaac atgatgaaga
     2281 tagaggattt tgcataaatt ataatgacta tgttccagta tttaataagt ttaaagaaaa
     2341 atataaaaag gacatagagt tattactagg tgttgaagtg ggttacagaa aacatctaaa
     2401 aaatgaaata gaagaaataa taaattctaa tccttttgat tttgtattgt gctcaactca
     2461 tacaattgac aatgtacctg ttccatcaaa ggagtatttt aaaggtctta gcaaagaaga
     2521 tgcgtattat aaatatttta atagcatact tgaaactaat cgtgaatttg gagattacaa
     2581 tatatatggt catttggatt atatatctag gtatggaata tattcagata atagagttat
     2641 atacaatgac tttaaagatg ttattgatga ggtcttaaag tcaataatca ataatgggag
     2701 tggtatagag ctaaatactt ctggttatag atatggatta aatgctatac atccaaatga
     2761 agatattcta aaaagatata aagaacttgg aggatttata gttactgtag gttctgattc
     2821 tcatagggta gaagatatat gtaaagattt tgatgtggcc tatgatatgc ttaagtatct
     2881 tgactttaaa tatgtatctt tatttaaaga aagagaaact tattttgtaa atatagaaaa
     2941 agttaaatct aacaatatag cttagagtgt gttttgaagt aaattaatgg atagttaaaa
     3001 agttaatagt tataatattg tatcctctag aaaaatacta gaggttttta ttttgctaaa
     3061 ataactaaat agaattaaat ttaaatagta tagtttattt taaattgaat agtttattgt
     3121 tgatatattt tactattata tatttttatt gtgatataat agtaaaatat attaacctat
     3181 ctttagatta tataaattat tataaaggag ggaaatttta aaatgtctaa taaaaataaa
     3241 gaaaatataa ctttaagtaa tcaaatcaaa ggaacaattc aaaatagttt aacaaaagaa
     3301 gctatattac atataccaat gagcaattat gcctatggtt atgatagaga aacactacat
     3361 ataagactta gaactaaaaa aaatgaagcg aaaagagtaa ttttacgaat aggagaccaa
     3421 tatgtgtggg ataaaggagg tgcaggagga ggaaatttaa atgcatctgg agttggttgg
     3481 tctggtggaa caaatatagt aatgagtaaa gaagtagaaa ctgaattatt tgactattgg
     3541 atagctaagt gtaagccttt aaataaacgc tcacgatatg gctttataat agaaggtcaa
     3601 gaggaaaaac ttttatttac agaaaaaaga ataatagagt tgggaaatga aaatgatgaa
     3661 aaggaattat gtgaaatagg taattttttt ggatatccat atttaaatta tatagatata
     3721 cctaaagtgc caaattgggt gaaagatact gtttggtatc agatatttcc agatagattt
     3781 gcaaatggtg acccgtctat aaacccagaa ggagtagata aatggggagc tattccaacc
     3841 agagataatt ttacaggtgg agatttacaa ggtgtgatag aacacttaga ttatttgagt
     3901 gatttgggaa taaacggaat ttacttttgc cctattacaa ttggaaaaac caatcataga
     3961 tatgatacgg tagattatat ggaggtagac cctacattag gagataaaga gactttaaaa
     4021 aaactaatag aagaagcgca taagagaaat ataaaaataa tgttggatgc tgtttttaat
     4081 catataggat attattctaa acagtggcaa gatgtagttc aaaataagga aaattctcga
     4141 tataaaaact ggttttatat aaaagatatg agtaaaattg atactccaat agaacaaata
     4201 gatgaaaaaa atatacctta tgaaaccttt gggtgtgaaa aatatatgcc taaacttaat
     4261 acagaaaatt cagaagtaat aaaatattta ttggatgtag gcaagtactg gatacaggaa
     4321 tttgatatag atgcgtggag attagatgtt tcaaatgaag tagaccatgt tttttggaga
     4381 aagtttagag aagaagtcaa aaaagtaaaa ccagacattt atatattggg agaaatttgg
     4441 catggtagtt taccatggct aatgggtgac caatttgatt cagttatgaa ttacttgatg
     4501 tcagaagcca tgaaaaaatt cttttgtaca gatgagataa acgccgaaga atttaagtat
     4561 atgataaatg atgtaatggt aagttatcca atacaagtta gtgaagttat ttttaattta
     4621 cttggaagtc acgatactac aagaatattg acttatgcta atggaaatat tgatagattt
     4681 aaacttgcat atatgtttat gtttgtacaa acaggatgtc cttgtatata ctatggtgat
     4741 gaaatcggaa tggaaggaga attaagttca atatctgaag gtcaaagaaa atgtatggaa
     4801 tggaatgaag aaaaatggaa taaggatata tttgatttta tgaagaagat tattagactt
     4861 cgtaaagaaa ataaagaact taggtctata tcaaatgaat gggtgttagc agataaggaa
     4921 aatggaacga ttatattgaa aaaagaatct atttctataa taataaataa ttcatctaaa
     4981 aatataattt tgacgttacc agattattta gcaaataaaa aagtaaaaga tttatatgaa
     5041 gaagaaatag ttgatttaaa agaagaacta atgttagaga aatacaagtt cattatttta
     5101 aaataataca aaaaagtatt tattttagat ttcatgttaa attattatac agtttaaata
     5161 agtctttata aacgataaaa aaatatggtc tacaagtgag tgaattgaaa gtagatttta
     5221 aattaaagac tgagttagag tagataattt tataaataat atcttaaata caagatagaa
     5281 aataaattga ttattagtct aaaaatttct tgtagaaaga ggaaataaga atgattgaag
     5341 catttatatt tgatttagat ggtgtaatta cagatactgc atattatcac tatatggctt
     5401 ggagaaaact agcccataaa gttgggatag atatagacac aaaatttaat gaatctttaa
     5461 aaggcataag tagaatggag tcattggaca ggatattaga atttggaaat aagaaatatt
     5521 cattttcaga agaagaaaaa gtgagaatgg ctgaagaaaa aaataattac tatgtatcat
     5581 tgatagatga gataacttca aatgatattt tgccaggtat agaaagcttg ttaattgatg
     5641 taaaatctaa taatataaag ataggtctat cttctgcaag taagaatgca ataaatgtat
     5701 taaatcattt gggtataagt gacaaatttg attttatagc tgatgcagga aaatgtaaaa
     5761 acaataaacc acatccagag atatttctta tgtcagcgaa agggcttaat gtaaatccac
     5821 aaaattgtat agggatagag gatgcaagtg caggtataga cgctataaac tcagctaata
     5881 tgttttctgt tggtgttgga aattatgaaa acttaaaaaa agcaaattta gttgtagatt
     5941 caacaaatca attaaagttt gaatatatac aagaaaagta taatgagtat atagttagga
     6001 gaattatatg aaatgtttat ttagtaatgt agatgagtgg aagattatac aagataagat
     6061 tgaaatcaaa gaaaatagat tagcagagtc cataatgagt ataggcaatg gatatatggg
     6121 tatgagagga aactatgagg aaaattatag tggagataca cacaaggggt cttatatagc
     6181 tggagtatgg tttccagata aaactcgtgt tggttggtgg aaaaatgggt atccagaata
     6241 ctttggtaaa attcctaatt cagtcaacta tataggtgta agaattttca tagatgatgt
     6301 agaattagat ttagcaaaat gtaaagtaga agatttttat agagaattag atatgaaaaa
     6361 tggtatttta aatcgtaggt ttattgtaaa tataaatgat aaaaaatttg aagttagggt
     6421 tgctaggttt ttaagtatat ctgttaagga acttgcagta attaaataca gtataaaatc
     6481 attaaattct aatactaaaa taaaatttgt accatattta gattcaaata taaaaaatga
     6541 agattcaaac tatgaggaaa agttttggaa tggaatttct tcaagttctt tagaatataa
     6601 aggtagtatt ctttctcgaa caaaggagaa tgcatttaat acaccagtat tcactgtggg
     6661 ttcaatgatg tatattaaga caaatggtga tgtagaagct gtatctcatg agagtgatta
     6721 taattactgt gaaaattcga taacaatgga tgttttaaaa aatgaggttg caagtataga
     6781 aaaatatgta tctataactt caactagaga ttataaggaa agtgagctat taagaaaatg
     6841 tgaagacatt ttaaatagag aactatctaa aagttatgat gatatattaa atgaacatac
     6901 aaagttatgg aataaaagat gggaaagtgc agatgtaaaa ataaatggag ataaaagtag
     6961 ccaacaaggt ataagatata atttattcca actattttca acatattatg gagaagattc
     7021 gcgattaaat ataggtccaa aaggatttac tggtgaaaag tatggaggag ctacttattg
     7081 ggatacagaa gcgttttgtg ttccagtata tctaggtgta gcagataagg aggtaactag
     7141 aaatctttta gaatataggt acaatcagtt agaacaggca aaggataatg caaaaaaact
     7201 agggcttaaa ggtgctttat atccaatggt aacatttgat ggagtagaat gtcataatga
     7261 atgggagata acatttgagg aaatccaccg aaatggaagt atagtatatg caatatataa
     7321 ctatacaaat tatacaggtg actataaata tataaaagaa aaaggaatag atgtaataat
     7381 agaaatagct agattttggg caagtagagt tcatttgtct acaaaaaaag atttgtatat
     7441 gatacatgga gtaacaggtc caaacgagta tgaaaacaat atcaataata attggtatac
     7501 taattatata gctaaatggt gtttagatta tgctgtagaa aatatcttac gattagagaa
     7561 agagtgtaat gaatgtattg acagaaataa tgtgactagg gaagaagttg agttttggaa
     7621 aataatatca aataaaatgt atctgccgta tgatgaggaa ctacaaataa taattcaaaa
     7681 tgataatttt ttagataaag aatttgtaga agtaaaggat ttaccaaaag aaaattttcc
     7741 tcttaatcaa aattggtctt gggataagat tcttaggtcg tgttttataa aacaagcaga
     7801 tgtattacag ggaatttact attttggaaa tagatttact aaagaagaaa aaaagagaaa
     7861 ttttgacttc tatgaaaaat atactgtaca tgaatcttct ttatcttcta gtatttattc
     7921 tattattgcc tcagaaatag ataacctaga aaaggcttat gaattatatt caagaacagc
     7981 taggcttgat ttagataatt acaataatga tacaaatgat ggactgcaca taacttcaat
     8041 gtctggttca tggctttcca ttgtacatgg atttgcaggc atgagaacat ggaatgaaac
     8101 tctatctttt gaccctaagt tgccaaaaga gtggaaaaat tattctttca atataaatta
     8161 tagagataat attattaatg tagatgtatg tagtgattta attaaaatag aaaatttaaa
     8221 aggtagacca ataaaaatta ttgtatatgg gaaaaaatat atattagaaa aatgtataaa
     8281 aattgataaa tagatttaat aatattttta ttatataaat agttaaaata agctatctaa
     8341 ttatttattt attttaacta ttttgtgtga tagaaaacta tatattataa caatagtagt
     8401 gcttttaatt aaatatattt cataaatctt cataaaatct tcaaaatttc gtaaagaata
     8461 tgtcacaaaa gtaaggtata ttatattcag cagatagtat aagggaggac aatatttatg
     8521 aagaataaaa aattcattgt tataatactg ataatatcaa ttttaatagg tgtattagtg
     8581 aaggaatatt catctagaga gataaaaaag gatgatataa atgtcagtaa gtatataaaa
     8641 tatgctgatt tagcaagtaa aaataatgca caagttaact ggaagtatgt agcttccatt
     8701 gttgctgtgt taaataaaaa taacttaaaa aacgttaagg attcacagat acaagaagta
     8761 tcagatttat ttgttaagaa tttttctaaa aataataaaa taaataagtt aagtgatatt
     8821 ttggatgagt tagaatttag taatagacaa aaaagattgg ttgataatta tatagacaat
     8881 cttaaggatt atggtataaa accagaaaga ttgaagtctg atacaaaata tatgaagttt
     8941 attgatgaaa ttaaaacaga agcaatacaa aattataagg attataaaat acttccatct
     9001 ataactattg cacaagctat aatagaatct agctggggca agtcaacttt ggcaaaacaa
     9061 tatagcaatc tatttggtat aaaggctgac gcttattgga aaggtaagtc agtgacttta
     9121 gaaacgaaag aacatttaga tacaattata gatgataaat ttagaatata tgatgataaa
     9181 aatgaatcta taaaagacca tgcaaaattt ttagccacaa ataaaaggta taaaaacaat
     9241 ggtgtatttg atgcaaaaac gtatatatat caagctaaag cattagaaaa ggcaggttat
     9301 agtactgcga aggatgaaaa tggcaatagt atatatgcag ctcgtctaat agaattaatt
     9361 caacagtata atcttcaatt aatagacagt gaaattcaaa gtgaaatgta actttgatta
     9421 aatttttatt gataaggcta tgatttttaa aatcatagcc ttattttgac atgcttttac
     9481 ttgtaattat taaatcgata tcattatctt tcaaaattta ctttatactg taaatcaaat
     9541 tatttaagtt aaaacttgga attatttata aaaggattat ttactttatc taaattctgc
     9601 acttaaagaa catcttttcc cttaagactg accgttagtc tataaaaatt ataattactt
     9661 ataaaaaagt gaatatatat ataaagtttt cagaaagact tttacttgta aattgaaatc
     9721 aatgaagtaa aaaaggattc tttcttaaaa gtaaataaca actagatagt gaataataaa
     9781 aaatggtaat ttgaaataat ttttatgaat agtattaata tatagataca gggagattga
     9841 tgcgattatg tatgaaaata ataaggtaat atacaacctt gaaagatgtg aaaa
//
LOCUS       DACULQ010000137         9881 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.138, whole genome shotgun sequence.
ACCESSION   DACULQ010000137 DACULQ010000000
VERSION     DACULQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.138"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(372..1529)
                     /locus_tag="JA154_14010"
     CDS             complement(372..1529)
                     /locus_tag="JA154_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HAT7996960.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            1683..2252
                     /locus_tag="JA154_14015"
     CDS             1683..2252
                     /locus_tag="JA154_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAT7996961.1"
                     /translation="MKKEFRKKVIESRKNKNANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            complement(2434..2850)
                     /locus_tag="JA154_14020"
     CDS             complement(2434..2850)
                     /locus_tag="JA154_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996962.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(3076..4170)
                     /locus_tag="JA154_14025"
     CDS             complement(3076..4170)
                     /locus_tag="JA154_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996963.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            complement(4593..5534)
                     /locus_tag="JA154_14030"
     CDS             complement(4593..5534)
                     /locus_tag="JA154_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HAT7996964.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            complement(5527..6354)
                     /locus_tag="JA154_14035"
     CDS             complement(5527..6354)
                     /locus_tag="JA154_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAT7996965.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(6525..6887)
                     /locus_tag="JA154_14040"
     CDS             complement(6525..6887)
                     /locus_tag="JA154_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PemK family transcriptional regulator"
                     /protein_id="HAT7996966.1"
                     /translation="MSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQNDVGNKYSP
                     TVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREKIGCLDEN
                     MMIKVDDGLQISLGLFTF"
     gene            complement(6877..7164)
                     /locus_tag="JA154_14045"
     CDS             complement(6877..7164)
                     /locus_tag="JA154_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAT7996967.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(7197..8354)
                     /locus_tag="JA154_14050"
     CDS             complement(7197..8354)
                     /locus_tag="JA154_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAT7996968.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(8369..8959)
                     /locus_tag="JA154_14055"
     CDS             complement(8369..8959)
                     /locus_tag="JA154_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996969.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(8980..9441)
                     /locus_tag="JA154_14060"
     CDS             complement(8980..9441)
                     /locus_tag="JA154_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996970.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(9456..9836)
                     /locus_tag="JA154_14065"
     CDS             complement(9456..9836)
                     /locus_tag="JA154_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAT7996971.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRDEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
BASE COUNT         3158 a         1854 c         1047 g         3822 t
ORIGIN      
        1 gtttaattta ttttcaatgt ttaaaaaagt tattctatgc atctaattta attagtaaac
       61 tgaggttcta gttagataaa ttatattata atgaatatat aatatgcagt ttgcgatatt
      121 ttaaaagtaa atgatataat atttggaaca actgggttgc aatatgtttt taaaaaatat
      181 caaaataagg ctatgatttt taaaaccata gccctgtctg taaaaattta attatcactt
      241 aaaaaataaa tttttattta aagaatacac ataaatattt ccagactccc tcatgaacta
      301 aaaagttcat tttgagacat ctcttttata atgttcttta ttttatagta taagtttata
      361 aaaattaaat cctatttatc aatctgttct ctaagccact tagttgctac atctaactgt
      421 ttattttcat ctttctcttc aatagtaggc tttatacctt tttcattgat ataatcccca
      481 ttaggtgtaa aatattgagc agtagttagc ttgtatcctg ttccatcctt caatcttaca
      541 actgattgta ccaacccttt accaaaagtt gtagtcccta cagaaatacc ttctttatta
      601 tcaacgattg cagctgttaa tatttcagca gcagaagcac tttctccatt agttaacact
      661 actattggaa gtcccaattt ttctttatca gatttcaagt actcagtatt tcccttatta
      721 tcttttgtat atactatagt tccttcacca attaactcat cagcaacttc tttacatata
      781 tctagcattc caccaggatt atttctaagg tctattacta atcccttgat attttttgct
      841 tttaattctt ttaaagcttt atcaaagtca gtatatgtac ttgatataaa ttgggtaatt
      901 tctatatatc ctaaatcatt atcaattact ttactttcta tactcttctc aattatctgg
      961 tctgttttta ctttaaagtt caattgttta tcttctctta atatagtcag ttcaactgtt
     1021 ttaccttttt tacccttcat catacgaaca gcttcatctg aatttttata tgagacagat
     1081 tttccattga cttttactaa tttatcccct gattttacac cggctttctc tgctggagaa
     1141 tctttcatgg gaactactac taagccacca tcttttgatg cccctatata catacctaca
     1201 ccaacataag aaccttcact atcattaatt agattctcca tttcatcttt agtataataa
     1261 ccagaatact tatcatttgt tcctagaaat agaccttttg ctgctccatt taccaaatca
     1321 gtatctgata cttttttata aaaatcttgt tttataatag attctagtcc taacaactta
     1381 tcatattttt tatagtcttc atagagttct ttcgatatta caactttatt ccccaatgtc
     1441 aactgaaatg ctgatgtaac catagctgtt ataattacta aaatcacccc taaaaaaata
     1501 gctttcttct ttgaaatcac aagatacatc tccttttaca taaactatta ttattatatg
     1561 actagctgtt atttttatta catttaccca aatatattgc tttattacaa taaattatac
     1621 taaaatttat attaaataca attgctgtac acattataac atattaaggg aggaattatt
     1681 ttatgaaaaa agaatttaga aaaaaagtta ttgaatcaag aaaaaataag aatgcaaact
     1741 ttgtacttag taattcaaat ttaataactg aaaaattact tcaaatggaa gatataagaa
     1801 atgcaactaa tataatgctt tatctaaatt ttaacaatga agttcaaaca gataatttaa
     1861 tcaaaaaact tttgtctcta aagaaaatag tatcaagtcc catcacaata aaagaaaatc
     1921 atacattaat ccctactcaa ataacagatt taaaagaggg attgagaatt ggagcatatg
     1981 gaataagaga acctaacgaa aaatcaccta caattgatat taaggcctta gatgttgtaa
     2041 ttgtaccagc tgtagcatat gatatacatt gctatagatt aggatatggt ggaggatttt
     2101 atgatagatt tttagaaaac ttaagaaaag atgctataac tatcggcata gcatttgact
     2161 tccagatatt cgattctatt ccaaaagaaa ctcatgatgc acaattagac tacatagtta
     2221 ctgaaactag aattatcact ccaaatagtt agaatattaa gtatttccag accccattta
     2281 atgtagtaat aaatactact gttaatatct tactttataa gcattttaag tcatggcttt
     2341 tacaacaata agaaccatac acaatttact tacattaata aaaaccatga ctttattgta
     2401 caatatgcag ttatttaatt ataaaatacc ttcttatttc gtcgcattta attctattct
     2461 atctggaagt tcactcatat ttgtttcaac aactatgtat ggaaaagaat cttccttatc
     2521 tttatacttt atcttgactc tcaaaacatc ctttccttcc tcaaccacac tttctatttt
     2581 gtccatttct ataccatatt tattatttcc tctagtcact ataatgtata ttttgttatc
     2641 gattttagcc gctaatgctc tttcttcatt tgtatactta tccatcacat ctaatatctt
     2701 ttctggtatt gaatttcttt gtaatactgt ataatctatg ggtttagatt tctctacaaa
     2761 catacttggt atgaatataa atccccctat aacgaataca agagctataa ctccataaat
     2821 tataccttta atattaaact ttacattcaa tcttatcact cctcccatat attctaaata
     2881 tatgatttat aaacttatct tagactctaa attattgatt agtatatatt tatacataat
     2941 tataaatata tgtaatgtaa ctaaaaaaca tgttttttct atttcataat aaacgagcta
     3001 aaaaataaaa agagctatat gaaaacattt tcattgtttt catatagctc ttgagtttac
     3061 aatattaaaa aatacttatt tgtcactgat tccttcttga tatccagatg tttttgtaat
     3121 ctgtctataa agaacacaaa ccagtattat gacgcccata tatccagtat atggatataa
     3181 aataccaacc agcttatcaa atggtaggaa gccacctata agacctaata tagctgctat
     3241 taaagttatt aatttaaact taggatgttt gtcatctaca aatctattag ttactgacca
     3301 taataaaggt acagctgttg tatatatacc taatataaga attattgaga ataaaactcc
     3361 aattactggt gaaatcttat ctgctaagaa taatgatggt attgcttttg aatataagtt
     3421 tcctatatca gaaagaatag ctaagtgcat cattatagct gcagccataa gtgctactcc
     3481 acctaatata ccaccacaaa tagcttcttt tttgttagca gcacttgctc ctaatccagt
     3541 taagaatgcc ataacaacaa ttgtgttata agtagtatat attacaccag atagataggc
     3601 atttggaact ggtttagcta tctttacagt gctaagaact tgtgcagctt ctcctatatt
     3661 acctatgttt ttagctaaac ttataccacc aactaaaagt gtaaagatta ttattatagg
     3721 ccctattcca cctagtatct ttgataattt atcaagacct agtgaaacag ttaataatgc
     3781 tactactgcc atacctactc tacctacaaa tggatttaat ccatagtatt cagttaaagt
     3841 tgctcctgct cctgaaatca ttactacgaa aacaccaaat aagaataaag gtccaaacca
     3901 ttcaaagaat attcctaaat acttaccaca ataaacacga taaattttga taggttcttt
     3961 aagttgaagc tctttacctt ttgacataac tgaagcaccc atccatgaga ataataccat
     4021 tgaaattatc ccaccaataa tacctgcaaa accataaaaa gagaagaatt gcattatttc
     4081 ctgtcctgtt gcaaatccag aaccgattac tgttgctaca tacgcacctg cgaaactgat
     4141 gactgtttta acatttactt tttctgacat atctcagtta cctccccatg tgaaattttt
     4201 tcttctgatt aaacaattcg ataaactacc tacaatttcc tttaacataa aatacatgat
     4261 aactgtttta tagcttttta gtataataac ttaaaactta taatctgatt ttatacccta
     4321 ctcctcctct cataattttt ttagattctt ttatctaagt agtcattata tataaaatta
     4381 gtttctctat aaattatata ttagtcatat ttttctgaat agtcaatata tatttgaata
     4441 ttttctctat ttttgtaaaa atttgaatat tttattacat ttttgatata ttgatatcat
     4501 ataaaaaaaa gagagatata aattaatata acactttata cctctctttt aaatatatta
     4561 tattattaac aaacatgaat aatttatatc tcttacattc tttttgcaat aatagcttct
     4621 ttaacagtct ttattatgtc atcacaagtt aattcatatt tttttaacaa ttcatttgga
     4681 gtaccagatt ctccaaatgt atctttaatt ccaactttct ttacaatggt tggacaagac
     4741 tctcctacta cctcacttac agcagaacct aatcccccaa ttatgctatg ttcttctaca
     4801 gttacaatag cattcgtttc ttttgctgat tctaatatta aatctttgtc tataggtttt
     4861 atcgaggaca tgtttataac tctagcactt ataccttctt cttgcaactt ttcttgtgct
     4921 aatagagctt catttaccat aattccacaa gctataattg atacatcctt tccttctctt
     4981 aagactgtac cttttcctat ttcaaattta taatcttcat caaataaaac tggtactgaa
     5041 cttctcccta atctaacata aacaggtcca ttatacttag ctatttcaaa aataattttt
     5101 tctgtttcaa ctccatcagc tggaacaaga actgtcatat ttggtatagc tctcattata
     5161 gcaatatctt ccacactttc atgagatgct ccatcctctc caacagtaag acctgcatga
     5221 gtagcacata tttttacatt tagctttgga taacaaactg aatttcttat aatctcaaaa
     5281 gcccttcccg tagcaaacat agcaaaagtg cttgcaaaag gtattttacc agcagttgat
     5341 aatccacaag cagcgcctat taaattttgt tcagctatac ccatattaaa aaatctatct
     5401 ggaaatgact tgtaaaaatc atgtgttttt gtagactttg ataaatctgc atctaatact
     5461 acaacattgt catttatttg acccaatttt actaatgctt taccatatgc ttctctcgtc
     5521 gccattttac tcattgtcag cacctcctaa ttcaagcaag gctttttcta gctcctcatc
     5581 acttggagct gtcccatgcc atcctgcttg attttccata aaagatattc ctttgccttt
     5641 tatagttttt gcaactatca ttgtaggttt tttaactgtt gatttagcta tatcaagagc
     5701 cgcaaatatt tggtcaaaat catgtccatc tatctcaatt acattccaac caaaagcttt
     5761 aaatttatca actattgaac ctaaactcat tacgctttct atatctccat ctatttgaag
     5821 tccattatta tctaaaaaag caattaaatt atcaagttta taatgtgcag cactcatagc
     5881 agcttcccaa acaattcctt cttgaacctc accatcacca agcaaagtat acacattcca
     5941 cggagcatta tccaactttg atgccatagc cataccacat gcaactgaaa aaccttgacc
     6001 taaagaacca gtagatattt ccacacctgg cgtgcctttc atgtctggat gaccttgtaa
     6061 catccttcct atcttcctta atccattcag ttcttcctta tcaaaataac ctttttcagc
     6121 caatgtagca tataatgctg gtgcagcatg tccttttgat aatacaaatc tatctctatc
     6181 ctccatttta ggatttgaag aatccacatt catttcatca aagtataacg cagtaagtat
     6241 ttcaactact gataatgacc ctcctggatg tccagatttg gctctatgta tcattgaaac
     6301 tatatctctt cttatgatac gcgcaatctc atttaatcct ttatggtctc tcatgcaatt
     6361 acctcccttg agttcgtaat tttatatcaa ttccatatta ttatatcact tttatgctat
     6421 ttttttgcgt tatttttaat tttattaaca tagtacatta aaattaaacc ttaagagata
     6481 tttaaaagaa aaaagggatt gtctaataaa agacaaatcc ctctttaaaa agtaaacaat
     6541 cctaaactta tttgaagtcc atcatctact tttatcatca tattttcatc tagacaacca
     6601 attttttccc tcagtctctt tttatcgata gttctaattt gctctaaaag aattactgag
     6661 tctttattaa gcccatattc attggaactt atctctacat gagtaggtaa cttggcttta
     6721 ttaatctgag atgttatagc tgatactata actgtaggac tatatttatt acctacatca
     6781 ttttgtatta tcagaacagg tctaacgcct ccctgctctg aaccaacaac tggacttaaa
     6841 tcagcgtaat ataaatcacc acgctttatt tcacaattag tactcacctt gggcaatctt
     6901 cccttcattt ttatcatcag aagatacttc ttcaactgtc atacctaatt cagctagtga
     6961 cagattaatt tgacccattt ctctataacc cgttttcata gattctttaa tatgaatttt
     7021 ctttttctga gttatataga actgaaaagc agcttttgca aaatcttctc tgttactgtt
     7081 ctccatctga actatactat caacttcaga aatcagagag tcaggtaaag taacttctat
     7141 tttttcttta ctcttatcgt acacaaaatc gcctcccaga ttataatatc atggttttat
     7201 tttagcaaat aactgtttat ttgtacaagc tcattatttt ccatataaac acggtcaact
     7261 cttcttgata tcatacataa cacttcatag tttatagttc ctaagtcttt agctatacgc
     7321 tcagctgtaa cttcgccttc tccaaatagt ataacctcat ctccaacttt tatgtctata
     7381 tctttgtcaa ttctaaccat tatttgatcc atacatattc taccaactac atcaaacact
     7441 tctcccttaa taagaacctt tggatttttt tggattctag taaatccatc tgcgtatcct
     7501 attggaactg tagctattgt ttctttacct gtagttgtgt attttagtcc ataacttatt
     7561 cctacacctg gttcaacttg ttttatatgt ccgatttttg attttaattt catggctggt
     7621 cttaattcta atctatcttt aaatacatca tcagatggat aatgaccata taatattatt
     7681 cctgctctta ccatatttaa tcttaaatca gggcaatcca taatagctgc actgtttgat
     7741 acatgtttta tagctatttt tacaccagcc tcatctaatt tatcagacat aaatttataa
     7801 ttattagctt gtttataagt gtactcttta cttacttcat cagctgtagc aaaatgagta
     7861 aacatacctt ctaaatctat atattctaat ttatttaatt ctattatttc ctgtactgac
     7921 tcctcattag gttggaaacc tattctagtc atccctgagt ctattttaac atgaacacag
     7981 gctttttctc ctaaagattt tgcaatttca tttatctttt gtgctgtttc taaagaataa
     8041 actgtcatag ttattttatt ttttatagaa tcttcaaaag cttcgtctgg agtatatccc
     8101 aagttcaaaa taggaagtgt tatgccattt tgtctaagtt caattccttc agcagttctt
     8161 gctactgcta agtaatctac tttttctttt tctagcaatt ttgcaacttc tactgcacca
     8221 tgtccatatg catcagcttt tattactcca caaatcttaa tatcttcttc taataaattt
     8281 ttaatattat ttaagttaaa tcttaagtta tctagattta tctctgccca tgtaggcact
     8341 gttatttttt gcatgtcttt agtccccctt agtttctgta ttcaaaatct ttgtatttta
     8401 ctgtaaatct tggcactccc tctttatcta acacttccat tttttcagga ttttttgtgt
     8461 cagcatttac atataatact tgcttgttaa aatatttatt atccccagga ataaaagtct
     8521 ctaaaaccaa atgtcctttg gataacttta ctttcatctc ctcattttga agatagtttt
     8581 ttataaagtc tcctacaaaa agatattgat tattttttcc tgtattaggt aactccacta
     8641 catcactaat ctttggattt ttaactaata ttttatcacc ttgatattct atacttttcc
     8701 cttttaaatg ttttggggaa attacttcta atttatagtt atcggttttt ttataagtat
     8761 gaattaaaac ataattgtgt ggacttttat ttccaactac ttctacttca gctttacaac
     8821 tgtaataatt catatcagaa atctgctttt gaaaatcttt gtatacttct tcttttgtgg
     8881 actgtcgttt ttggcatcct attacaatta taaccagtgc caagaacata atacatacta
     8941 tggtccactt ttttctcaca tcacacctcc tacttcagtt tattcattgt ataaagcttt
     9001 tattatatca tatctacaaa ttataccttt aagtttgttg tcatcatcta ctacaggaac
     9061 tctgtttatt gattttttaa tcattacatt agctacatca tcaaacttat catcttcatg
     9121 aacctttatt atatctttag acataagttc tccaacctta taagcagcta cttgttttat
     9181 atctttttca acttttttta agtcatctaa gaaaatcaat ccttgtaata gatttatata
     9241 gagaggtgct tctatatatt tttctttttt aagaatatct gtttcagaaa ttattccaac
     9301 aactctattc tctgaatcta caactggcag acctcctatt ttttctgcaa ttagcatatt
     9361 agctacatct gctatactat catcttgttt tgctactatt acatctgttg tcattatttc
     9421 ttttgctgtt ttatccatca taatgtaatc actccttagt ccttaattat ggttatcgca
     9481 tttgctatag cataatcttt tgaatgtgat attgaaactt ttatttctaa aacattataa
     9541 tctatgcaca tttttgctag attattataa gtttttacta ttggttttcc catttcatct
     9601 ctgagaactt ctatatcttt aaaattaaac actctaattc ctgtacctat agatttactt
     9661 atagcttcct tggctgcgaa attacctgct atactttctg ctttaaaatt cttagaatta
     9721 aaatactcta tttctctatc tgtaaatatc ttctccaaaa acctattatt tttgtctaca
     9781 gcctttctta ttctatcaat ttcaattata tctacgccaa tatcaaatat attcattgtt
     9841 cctccagaaa atttataata cttctttcaa taattaataa t
//
LOCUS       DACULQ010000138         9878 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.139, whole genome shotgun sequence.
ACCESSION   DACULQ010000138 DACULQ010000000
VERSION     DACULQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.139"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            163..1230
                     /locus_tag="JA154_14070"
     CDS             163..1230
                     /locus_tag="JA154_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HAT7996972.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1400..3301
                     /locus_tag="JA154_14075"
     CDS             1400..3301
                     /locus_tag="JA154_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HAT7996973.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAECIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3294..3767
                     /locus_tag="JA154_14080"
     CDS             3294..3767
                     /locus_tag="JA154_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HAT7996974.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3742..4968
                     /locus_tag="JA154_14085"
     CDS             3742..4968
                     /locus_tag="JA154_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAT7996975.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(5107..6294)
                     /locus_tag="JA154_14090"
     CDS             complement(5107..6294)
                     /locus_tag="JA154_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7996976.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6567..7391
                     /locus_tag="JA154_14095"
     CDS             6567..7391
                     /locus_tag="JA154_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAT7996977.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7520..8785
                     /locus_tag="JA154_14100"
     CDS             7520..8785
                     /locus_tag="JA154_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HAT7996978.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8998..9588
                     /locus_tag="JA154_14105"
     CDS             8998..9588
                     /locus_tag="JA154_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HAT7996979.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
BASE COUNT         3852 a         1063 c         1775 g         3188 t
ORIGIN      
        1 tacaataaag tttaatctgc ttatattttt atttatttta aagaagttta ttttaaaaat
       61 aaaattaaat ttaaaaagtt atgtattgat tatatttaaa aatgttatac tctaaaaaag
      121 tatattttat ttaaaaattt attaatacaa aggagattta ctatgaaaaa taaattagtt
      181 tacatatgta ttatgagtct attgctattt gtagtaggat gttctaataa tccaaataag
      241 gaaagtacca ttaaaaaaga ctctaagaat aaccaaagcc aagttaaaga agaaaaaaga
      301 gaacctgtga aaaaagatat aactataagt ttcgctggtg atgtaactat ggggaactat
      361 aaaggttcat catattatgg cagttttgac catgaattta aaagacaagg agaagattac
      421 aattattttc ttaaaaatgt aaaacctgta tttgaaaaag atgacttaac agtggttaat
      481 ttagaaggtc cacttactac agcgtcaaat gcaaaagaaa aaaaatttgc atttaaagga
      541 aagcctggat atgtaaatat tttaaaatct ggaagtgtag aggcagtaag tattgcaaat
      601 aatcattcag aagattattt tgaagaagga atgaaagata cacagtttat attggacgag
      661 aataaaataa attattttgg attagggaaa gatgctgttg tagatgtcaa aggaattaaa
      721 gtgggtctat tagggtataa tggattatca actgaatata atgaaaaaaa tttaaaacaa
      781 atggaagatg atattaagtc cttaaaaaag aaatcggatg ttgttgtagt gtactttcat
      841 tgggggatag aattagatta ttatccagat aaaaaacaaa aagattttgc tcattatgct
      901 atagacaagg gagcagattt agttatgggg tctcacccac atgtgattca aggaatagaa
      961 aagtataaaa ataagtatat agcttatagt ctagggaatt tttgctttgg aggaaataag
     1021 aatccttctg atacagatag ttatatatat caacaaacct ttactttttc agataataaa
     1081 ttaacttcaa ttaaagagcc aaatataata ccaacttcaa taacatctag taactccaga
     1141 aataattatc aaccaaaaat attggatgga agtgagaagg atagagtttt aaataaactt
     1201 gaaaaaataa gcaaagattt aaataaataa aatgtaaaaa aaaataaata tatatattta
     1261 ttttaataat ttatattttg tttagggtat ggtaaatcgt ggtattaaat aagtagattt
     1321 taatagttaa attatagtca attataataa agctatttaa tctacttttt taatataaaa
     1381 atataagggg gaatttgtaa tggcttgtaa cacttgtaaa atgtgcgaaa gtgcagacaa
     1441 gaaattagaa agttttgtag cttcaaagga tgttgaaaca gcatttcata gaacagaaga
     1501 tcagaaagtt aagtgtggtt ttggacttca gggggtttgt tgtagactct gttcaaatgg
     1561 accatgtaga gtaactccta aatcaccaag aggtatatgt ggagcagatg cagacactat
     1621 agtagcaaga aactttttaa gagcagtagc atcaggagca gcttgttatt tacatgtggt
     1681 agaaaataca gctaagaatt taaaaaatgt tggaataact aaaggtgttg ttaaaggaga
     1741 aaaaacatta aatcaattag cagagatgtt tggaatagaa agtgatgaaa agtatgataa
     1801 atgtataaag gttgcagaca aagttatcaa cgatttgtat aaatcaagag atgataaaat
     1861 ggaattagta gaaaaaatag cttatgcacc tagagtgaaa aagtggaaag agttaggtat
     1921 aatgcctggt ggtgcaaagt cagaagtttt tgatgcaata gtaaaatcat caacaaattt
     1981 aaacagtgac ccagtagaca tgctagttaa ttgtttgaac ttaggtatat caacaggatt
     2041 atatggtctt acactaacta atcttttaaa tgatgttatg ttgggagaac cagttataag
     2101 aatggctcct gtaggattta atgtaataga cccagactac ataaatataa tgataacagg
     2161 tcatcaacat tcaacatttg caaatttcca agatagatta aaagatgaag atgttataaa
     2221 attagctcaa tctgttggcg caaaaggatt taagttagta ggatgtacat gtgttggtca
     2281 agatttacaa ttaagaggag aacattatca agaagtattt gctggacatg caggtaacaa
     2341 ttttacaagt gaggctgttt tatcaactgg agcaatagat atagttcttt cagaatttaa
     2401 ctgtactata ccagggcttg aaccaatagc agataaatat aaagtaaaaa tggtttgttt
     2461 agatgatgtt gctaaaaaag cgaatgcaga atgcataggt ttagacagaa gtaaattaga
     2521 tgaacttagt aacactttaa tagaaaaagc attggagtct tataaggaga gaagaggaag
     2581 tattgaaata gatataccaa aagaccatgg atttgaacaa tctttgactg gtgtaagtga
     2641 aaagaacttg aaagcgtttt taggagattc atggaaacct cttattaatt taattgcaga
     2701 aggaaaaatt aaaggtgttg cagctgttgt aggatgttca aatatgacag caggaggaca
     2761 tgatgtaaat actgttgaac ttactaaaga attgattaaa aaagatataa tagtactatc
     2821 tgcaggatgt tctactggag gattagaaaa tgttggtctt atgtcacctg gagcagaaga
     2881 attggcagga gaaaacttaa aagaagtatg taagacttta ggaataccac cagtattaaa
     2941 ctttggacca tgtctagcta taggaagact agaaatagta gcaacagaat tagcagctga
     3001 acttggaatt gacctaccac aattaccatt agtgctttca gctccacaat ggttggaaga
     3061 acaagcatta gctgatggag cttttggtct tgcccttggc ttacctcttc atttagcttt
     3121 acctccattt ataacaggag gaaaattggt aacagaagta ttaacagaaa aattaaaaga
     3181 cttaacagga ggacatgtta tagtgaatcc agaccctaaa tcttcagcta atcaattaga
     3241 agaaataata atagatagaa gaaaaaattt agggttaaag gatgtggaat gcaatgcata
     3301 gaatttttat aaataaagat ttatgtacag gatgtaagag ctgtgtacta gcatgtatgc
     3361 taaaacacaa taaagattat gacatgtaca ctcttgattt agaaaatata gataatgata
     3421 gtagagggca tatagaatta gatagtagac ataataatcc agttccaatt ctttgtagac
     3481 actgtgatga acctgaatgt gtattagcat gtatgagtgg tgcaatgcat aaagatagtg
     3541 aaagtggaat cgtttcatat gatgaagaga aatgtggttc atgttatatg tgtgtaatgt
     3601 cttgtccata tggattatta aagccagatg atagaagcaa gcagaacata cttaaatgtg
     3661 atttatgtaa agatgaggag tatcctagat gtgtagctaa ttgcccaagt ggagcaattg
     3721 aattacagaa ggaggaaaat gatgaattat gtagtgttag gggctagtgc agctggtata
     3781 aatgcagtaa aaacattaag agaattagat aaagattcaa atatagtagt aatttctaaa
     3841 gatgaaaacg tatattcaag atgtatgttg catcacgtta tatctgaaca cagaacttta
     3901 aaacaaataa attttgttga tgaagatttt atggagcaaa ataatgtaaa atggattgca
     3961 ggtaagactg ttaaaggtat agacataaat aaaaaggtag ttcagacaga agacattact
     4021 gtaaattatg ataaactttt aatagctaca ggtgcatctt ctgctattcc tcctataaaa
     4081 aatttaagag atggaaattt tgtatattct gtgagaaaca tagatgatat ttataaaata
     4141 aaagaaaaag cacaaaattc taaaaatgta gttataatag gtgctggttt agttggtata
     4201 gatgcattag tgggattatt taaatatgag cagttaaata tttctgtagc ttttatggaa
     4261 aagtacattt tagatagaca attggatgaa tatacagcat cagtgtatga aaataagttc
     4321 aaagaaaaag gtgtaaaatt ttacccaagt gcatcaatac aagaaattgt attggataac
     4381 agtaaaaatg ttacaggtgt tgcattttca aatggtgaag ttttagatgc agacatggta
     4441 attgtagcta caggggttaa accaaatgct gactttttag atggaacagg aatagaatat
     4501 gatagaggta taataataga tgatatgtgc caaacaacac aaaaagacat atatgcagca
     4561 ggagatgttg taggtaaaaa tgctatttgg cctctggcag taaagcaagg aattgtagct
     4621 gcctataata tggttggaaa agataagaag atagaagatg aatttgcttt taaaaatagt
     4681 atgaatttca tggatatacc aactatttct ataggaatga atacacctgt agatgatagc
     4741 tataaagtat taaccagaca tggaattaat gattataaaa agtttgtttt taaagataat
     4801 gtaatctatg gagcagttat acaaggagat atttcatatg taggtgtatt aacttattta
     4861 ataaaaaata aagttgaaat atatgattta gaaaatagga tatttgatat atgttatgca
     4921 gactttttta atataaaaga aaatggagaa ttttgttata gtgtatagta tatacgtgga
     4981 gtaaggttta taaatacagt aaatcaataa atagagttat tctaaaataa gaaaaatgac
     5041 aaatcttatt tagaaatagc tctatttttt gtaattaaat ttaaattata ttcaatttgt
     5101 aaaatattat tttgaacaat tatacatata ctctctagtt agaggtaaat cattttttat
     5161 acctttagtg aaaagtattt gatgtatatc tataatacca gtgttgaaag cagcagcaca
     5221 agaacaaagg tataaatccc acattcttat aaacttatca tcaaacattt ctttaatctt
     5281 tggaagattc tcagagaaat ttttttgcca gtatagtaag gtttttacat aatgtaatct
     5341 taaactttct acgtctatag tatgatattt gtaatcacta caaatatgta tcatttctct
     5401 taggctagga ataactcctc ctgggaaaat atattttttt atccaagggt ctccataaga
     5461 ttcctgcaat ccacttatat agtgaagtaa aaacaatcca ccatctttta aaacagcatt
     5521 tacgttttta atgaaaagtt catagttttc tctaccaaca tgttcaagca tcccaacgct
     5581 tacaactcta tcaaacataa ggtctgattt ttttaattct ctatagtcca taagtcttac
     5641 ctcaagatag tcttgaaggt tctcttcttt aattcttgcg ttaaattcct taaattgctc
     5701 ttcgctaagt gtaattccaa cgccgtgaat tttatatttt tttgcagctt ctattagtaa
     5761 aaatccccaa ccacaaccaa tatcgagaag gctcatacct tcctctaaat ttagtttagt
     5821 taatatgtgg tgaactttat tgacttgagc atcataaaga gtatcattat catttctaaa
     5881 gtatgcacaa gaataactaa gtgtcttatc taaccataag ctataaaaat cgttgccaat
     5941 gtcatagtgg gcatgtattt ctttcttttg atgtctcatt gaatttgatg agtgcaatag
     6001 atgttttaca gctgaattat caatattgaa tttgtctatt tgttttaaaa gtagatttaa
     6061 agttaagaat aaatctccct caacttcaat gtctctattt atgtaggctt ctccaagtgc
     6121 taaagatgta ctttttaata gttcagactt gctaatatca ttatttacta taactttgaa
     6181 ctgaggggag ccctctccaa ttagaaattc ttcctcatct ttaagcctaa ctagaaatgg
     6241 tgtttcacca aatttctcta aatagtcttt taaaaaattt tttgtaatat tcatattacc
     6301 agctccttct aatgtaaatc cagtaagttt ccctgcttaa aatatattat agtccttttt
     6361 ttaaaaaagt aaaatttttt caataaattt tttatttaaa ggaaatttat tttttgtaaa
     6421 gaaatttcat gttgtagtat attttttgct tataaatttt ggtactggat ataatattaa
     6481 actgttaatg atataataaa tttaatctat aaagtaaata aaattttgta ataaatagtt
     6541 aaagctattt tataaggaga tatatgatgg aagtatatgc tttggttggg tcaagtggca
     6601 cagggaagag ctttaaggct ttagagttcg catatgaaaa tgatatagaa tatataattg
     6661 atgatgggat tttaatatac aaaaacaagg ttttagctgg gatatcggca aaacaagcaa
     6721 atactactat agaagcagtt aaaagggcta ttttttataa tctggagcat agacaagaag
     6781 ttagagaagc aattaaaaaa gaaaatataa gaaaaattct tattattggg acatcaaaaa
     6841 aaatggttaa tcaaattgta gagagattgt caattggaag ggtttgcaag tttataaata
     6901 tacaagatat aagtactaaa tatgaaatag aaatagcaaa acaagctaga aaagaaggaa
     6961 atcatgtaat tcctgttcca gcagttgaga taaaaagtat ggcaagtggt cttagtataa
     7021 attctttgaa gagacttttt agaaaaggaa ataataggaa tatggtagtt ttagaaaaaa
     7081 caattataag accaactttt agttatatag gaaagtttta tattagtgct gatgtaatta
     7141 aacagattat tgaatatgaa gtatacaact ttggaagcat tgatagaata aataaaatca
     7201 atatagagaa ttgcaataat tttatgaata tttttgtaag tataaatatc aatgatttag
     7261 aaactataaa aaaaattgaa gacatacaac agacaataaa aaagtctatt gagaaaatga
     7321 ctttagttaa tgtacaaaaa atagatatta acattcataa aattaaaaaa ataaacttcc
     7381 tccaatgtta aaaaataaac tgagaaaatg atatactaat ttgcaataaa gtgtgctata
     7441 attaaactgt aaatttagga aatggttttt tctaaatgga gtatacataa ttataacgta
     7501 tttataaggg ggctttcaaa tgtcaggaaa agatgtaaat gtcttcgaga tggcgcaatc
     7561 tcaagtaaaa aatgcatgtg ataaattagg tatggaacca gcagtttatg aattattaaa
     7621 agaacctatg agagttatag aggtttcaat tccagtaaaa atggatgatg gttctataaa
     7681 aacttttaaa ggatttagat cacaacataa tgatgcagta gggccaacaa aaggtggaat
     7741 aagattccat caaaatgttt caagagacga agtaaaagct ttatctatat ggatgacttt
     7801 caagtgttct gtaacaggta taccatatgg tggaggtaaa ggtggaataa tagtagatcc
     7861 atcaacttta tctcaaggtg aattagaaag attaagtaga ggatacatag atggaatata
     7921 taaattaata ggtgagaaag ttgacgttcc tgcaccagac gtaaatacta atggtcaaat
     7981 aatgtcttgg atggttgatg agtacaataa attaactgga caaagttcta taggtgttat
     8041 aactggtaag ccagttgaat ttggtggttc tttaggaaga acagctgcta ctggatttgg
     8101 tgttgctgtt actgcaagag aagctgctgc taaattagga atagatatga aaaaagctaa
     8161 aatagctgtt caaggtatag gaaacgttgg ttcttataca gttcttaact gtgaaaaact
     8221 tggtggtact gttgtagcta tggctgaatg gtgtaaatca gaaggttctt atgctatata
     8281 caatgaaaat ggtttagatg gtcaagctat gttagattat atgaaagaac atggtaactt
     8341 attaaacttc ccaggagcta agagaatatc tttagaagag ttctgggctt cagatgttga
     8401 tatagtaata ccagctgcat tagaaaactc tataactaaa gaagttgctg aatctataaa
     8461 agctaaatta gtttgtgagg ctgctaatgg accaactact ccagaggctg atgaagtatt
     8521 tgctgaaaga ggaatagttc ttactccaga tatattaact aatgctggtg gagttacagt
     8581 ttcttacttc gagtgggtac aaaacttata tggatactac tggtcagaag aagaagtaga
     8641 gcaaaaagaa gaaatagcta tggttaaagc atttgaatct atttggaaaa tcaaagaaga
     8701 gtacaatgtt acaatgagag aagctgctta catgcattca attaagaaag ttgctgaagc
     8761 tatgaaatta agaggatggt actaagattt actcttaata aataatagga tataattatt
     8821 taagatataa aggggctgta catagtacag cccttaaatt atgtggctta caatgaagta
     8881 agctattttt gttttaactt ataaataact tgataaatta tgtaatttag aatgtatttt
     8941 ttaccaccac atgattacaa attagaggag ttagattgaa tttttagaca tgaacttatg
     9001 cattttaaaa gcaaagacct taatttaaag tatctagtat tatttttaaa aatagtatat
     9061 tggtttaatc catttatatg tataatggaa aaatatatgg atttggattg tgaactttat
     9121 tgtgatgaga gagttttaaa aaattgtact gtacaacaaa agcaagaata tgctctaact
     9181 attgtaaatt caatgaaaag aagtacaaat ttttcaaata aatttgtagc tggattaaat
     9241 aaacaatctg atattagaaa aagagtaata aacatgttca gtgaaaaaca taaaaatggt
     9301 atcttaaggg ctttgattat aagcttgttt tcttctatta cattttttaa attcgattca
     9361 actagtatta ttgaagagaa tgatttctta gagaggtcaa gatatacgtt tgaaggaagg
     9421 caagaagaca tgtttgtaac aatagatttt atatatgcag atgtacctat aaaacttaga
     9481 aaagagcatg aagaaaactg tagagtcaat ggaatagtac caaaagattc agattcaata
     9541 gaaattattc ctagatttta taaagattgt atggatttaa aaaaataagt tagacttaat
     9601 attgatgttg atattatgaa ttttttatat ttaagcaatt gaaaatttta ttatagattc
     9661 aattttatat gtgtttaagt atgaaattta tagatagttg aaataattaa atatgattga
     9721 tgcataggaa tcattactaa gctataatta attatagggg agtttaaata aattatgagt
     9781 tacacaatca taaaatgata aaaatagtta tctgttacaa aaaatgtatc tttttaatta
     9841 ataaagataa tatgaaaact taaaaggcaa ataaactt
//
LOCUS       DACULQ010000139         9870 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.140, whole genome shotgun sequence.
ACCESSION   DACULQ010000139 DACULQ010000000
VERSION     DACULQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.140"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            32..913
                     /locus_tag="JA154_14110"
     CDS             32..913
                     /locus_tag="JA154_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT7996980.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKISNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            complement(1374..1538)
                     /locus_tag="JA154_14115"
     CDS             complement(1374..1538)
                     /locus_tag="JA154_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996981.1"
                     /translation="MSKKPIFALKLSTFVIALAEVVILKVFRFHNTTNTHRTAVRKTV
                     AWWVFSYAKK"
     gene            complement(1531..4098)
                     /locus_tag="JA154_14120"
     CDS             complement(1531..4098)
                     /locus_tag="JA154_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HAT7996982.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSNP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRAIKSGQMITVDGLAGTVTFDE"
     gene            complement(4101..4706)
                     /locus_tag="JA154_14125"
     CDS             complement(4101..4706)
                     /locus_tag="JA154_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7996983.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(5080..6540)
                     /locus_tag="JA154_14130"
     CDS             complement(5080..6540)
                     /locus_tag="JA154_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HAT7996984.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            6893..7480
                     /locus_tag="JA154_14135"
     CDS             6893..7480
                     /locus_tag="JA154_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7996985.1"
                     /translation="MIKMAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSK
                     SGIAKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKALKMLYRLY
                     DKNPFLYSPNNTDYITLLNKIPKEMLSQLEYSNVQSFYSLIERHNLKLKIEKEKAFGV
                     CNALLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            7531..7764
                     /locus_tag="JA154_14140"
     CDS             7531..7764
                     /locus_tag="JA154_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996986.1"
                     /translation="MIELIQRLNDIIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFLIILGIKVRKKFLIIIAIISIIINILIILDR"
     gene            7773..9023
                     /locus_tag="JA154_14145"
     CDS             7773..9023
                     /locus_tag="JA154_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7996987.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            9184..9753
                     /locus_tag="JA154_14150"
     CDS             9184..9753
                     /locus_tag="JA154_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT7996988.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3278 a         1710 c         1482 g         3400 t
ORIGIN      
        1 ttttaggtct ttaatgtcaa tagtgttaaa tatgccagca tatttatttg atagtaattc
       61 tattgcaaat atttacagag tatttcctat atcatttagc tttatagtta caggactata
      121 tttcagttat aaaaataata aaaataataa gattaacttt gaggcagttt tagaggacaa
      181 aaaaaactta attttcttat tgagccttat gtttggaatg tacttgtatc tatgtttgag
      241 tttgtcaata tactatactg ttggaaatgt attgatttta aaattatatg gaatcaagtc
      301 tgccataata gttttggtag gtaactatat tgcagaaaga tatagcttta gaattactca
      361 aataaaaggt tatgatatta aaaatcaaat agaatatgta gaaagtgtaa agacagagta
      421 tgagattaat aaattgaatg atattgtata tacagattta ctgacaggtt tctacaatag
      481 accttttgtg aatcaaaaac tatcagaatg gacatcaaat cactacaaat ttaccttatg
      541 ttttactgat ttaaatagct taaaacctgt aaatgataaa tttggacacc aatttggtga
      601 taaatacata tccactactg ctcaaataat aaaaaaactt ataaatgtag aaaattcctt
      661 actatttaga tatggtggag atgaattttt attcctttta cagaatacaa gttgtgtaga
      721 agcagaaaag ataatgatta atataaatta tgaattatca aaaataagta ataccgacga
      781 gtatccttat gctctttcca taagctatgg attagttgag tgggatggaa tatccgatat
      841 tgaaacttta atatcaaagg cagattcttt gatgtatgaa aataagctta aaaataaatc
      901 tactttaaac tagctagttc agatattcta caaaatatat attataagat agtactaaga
      961 atgtcaaaat atatagattt tgcaaatatt gaatacagtt tactaatgaa tgcactggat
     1021 gtaagtgtta gtaaacacct attagatgaa cactttactg ttgtttgggc taatgaccgt
     1081 tattatgaaa tgttcggcta tacctagcta aaaagttaaa aatgaccact gtagccgagg
     1141 gaatagagac tagttttcaa aaagatttct tgcaagaaat agactgtgat atgttacaag
     1201 gatacgtgtt ctctagacct ttaccaataa aagactttga aaaacttatg tttcaaaact
     1261 cctccaatta acaatataaa attgcttatt ataagtgata ttattgtatc aacaaataaa
     1321 ggaattgtct caaaattgat tttttctaat caaattttta ttttgagaca ttctcatttt
     1381 tttgcataag aaaataccca ccaagctacg gtttttctta ccgctgttcg gtgggtatta
     1441 gtagtattat gaaatctgaa aactttaagt attactactt ccgctagcgc aatcacaaag
     1501 gtcgaaagct tcaaagcgaa aataggcttt ttactcatca aacgtcaccg tacctgccaa
     1561 cccgtccacc gttatcattt gtccgctctt aatagctcgt gtggcaatgt gtgtagccat
     1621 aacggctggg atgccatatt cacgtgcgac aatgcttgaa tggctaagag gaccaccaat
     1681 gtccgttaca atcgcacttg cagatgaaaa taatggtgtc catgctggag ttgtggtaac
     1741 tgcaaccaaa acacttccag gttgaaagct ttcaaaatct gctggactat tcagtacaca
     1801 agctggagcc gtcacaacac cagtactggt accaatgccc gtcaacacaa ttttcccatc
     1861 tttctgcttc tgttgtgctt gtgatatagt ttttttgttt ttctctggca acttggatgg
     1921 tgacacataa cccatgtatt ttttaagttc agcttttcga acaggtatta tcccgttcat
     1981 atcagataga gacttgtttt tatccaactg ctctataagt acctccaatt ccgattttgt
     2041 gagccaataa atatcatcta aatgtgaaat agctcctcct cgtatgaatc gttctgagat
     2101 ttcctgaaac atacaacgaa tcagaggatg tcccattccc atcaaataaa tagcattttc
     2161 acgcatagga gaggtttcct gtgcccaatg gagcaactta aaaaatagtt ttttacgcaa
     2221 tccacctatg tgttgtaaaa tctcttcttc tgcatgtttt ctacgctttt caaacgtggt
     2281 ctggcgtaaa aaaggacttt ccccttttcc ttccacaaat gtttttacgg attcaaaagt
     2341 tggtgtaagt gtttcctgcg gtgtggaatg cgcaaaatca aactcataag cggtacgacc
     2401 aaactcttta aagtacttat tgattcgatt tttccattct atccatactt cctgtgaaac
     2461 tccaacaggc acaactgaag acatgaaatc ttctgctatc tttattgcag gactgttttg
     2521 cagataaagg ttgatgctgt tattttgctt tacccattct gaaatacccc acaactcctt
     2581 ttccgattgc agaacaatcg tatcaaaccc aagcaggaaa acggaagtat ccgttatacc
     2641 agcacgtcga actgctcctt gaaataattt tgtaaataat gtttcactga tagaagcagc
     2701 aggcaatgta agctgaatcc ttgtgaaata aatacatgcg gcataaaata ttgtctgaat
     2761 accctccata atttgatggg cgttcatagt atgcaggggc ttttcttccc attgtttaat
     2821 agcatcctca tatccttttc gtgccgtttc ccagcgtgca acgctgttca taagtgtcct
     2881 acgtaaagag cgtggggaaa gggatttgat agcaatcaaa agtggatttg atttagcaga
     2941 aagataaaca tatccattaa taaccgtata tgccccgttt tttggtaata attttttcgc
     3001 acttttacca aacatatcta tccataaaag ctctgatgca cggtttacga tttcaagacc
     3061 aagcgtggcg aacaaaggtg taacaggatt tggcaaatgt tctgccaaac ttccctttgt
     3121 gtatacaaca tcctgttctg gcaataccca ttctggtggc agaacagtaa ttggacgagc
     3181 ctgaacaatg tacaatttat ctttttccag tgcccactct acatccatag gcatttgata
     3241 atacttctca attttctttc caagctgtat cagttgcatt acctgattac gagtaagagc
     3301 atattttttt ctcaaccttt caggaaccat ggtttcttct gttccttctg aagtacgaac
     3361 cgtcataatc tctttatttg ccacttcata cgatataatt cgttcggagt ccttatccac
     3421 tacgattgta tcgggtgtaa ctaagctaga aaccaccgct tcaccaagcc cccatgcagc
     3481 gttaataatc atttcgctac gtctaccatt gattgggttt aacgtaaaca taataccaga
     3541 tgaatcggaa aatgcaagct tttgtactac aacagcaagt gcgacaatct cgtgctttat
     3601 attattcttc acacgataag caatagcacg agctgtccat aaggaagccc agcatctctt
     3661 tacagagtca ataactttat cttcaccttg tatgttaaga taagtttcct gctgacctgc
     3721 aaaagaagcg tcaggtaaat cttcagcagt cgcagacgaa cggacagcca ccgatatgtt
     3781 ccctaatcca gcataagcca ttttaattgc gtccgacact tcctgtggca tttctccatt
     3841 atggaaaagc tcccctattt tcttagatac atcttcaagt tggctggtat tattggaatc
     3901 aataccatct agcagtttat taattcgaga ctgaatatgg ttcgtctcaa caaaaatctg
     3961 gtatgaagca gtcgtaacat gaaaaccgtc tggcacaggg atgcctgctg tcaatagttt
     4021 tgataaagac atcccttttc caccaacaat ttcaatggtt gcttgtttat gttccagtgg
     4081 caagacataa taaatcataa tcattcacct ccatcattca taattccgtg cagaaatata
     4141 ttcattgttt catccaacat tttttccgaa ctatccaatg gtcgtgacgt gtagctgatt
     4201 gataacatgg agttaattaa caaacgtgtc acaagagcgg tatccgtttt gcgaaagatg
     4261 cctgtggtca caccattatc aattaccgat tttataatat cttgatatgc ataacgagca
     4321 tctttaagtc tctttcgata ctcttcttct aaataatcag attctgtatt taaccacatc
     4381 agtacagttc tatactgctc tggaaatcta agatgattaa gcatgatttt tctaaggcac
     4441 tgttgtggtg aaggttcacc atcaataatc ctatgaactt gttggatagt tttttctatt
     4501 tctttctcaa ctgcatatac aacaatctcg tctttcgttt tgaaaaaatc gtatagactt
     4561 gactttccca tgttggctaa aactgcaatt tctctcattg atgttttttg gaatccattt
     4621 tcagccataa gactcaaagc aacagcaact attttttctc ggcgttctgc ttgttgttcg
     4681 ggtgtaagca caattccttt tggcataggg cgatacctcc ttttagcgac cactggtcgt
     4741 ttctttttta tttatcatac agcgaccaac ggtcacttgt caaggttaat ttttttcatt
     4801 gtatatggta ctcgagataa aaatgttcat tttagaaatt gctctattta agtagaaata
     4861 ggcttcatca ttagaaaaca agactataaa tttgagatat ttatcatgtt caaatttcat
     4921 caatatcaaa gtttaagata ttaataaaaa aagaattact aagaaataac atataaagat
     4981 tcaaaaaagg agttgaactt aaaattcaat tacaaaaaat caaatttaag acaactcctt
     5041 tattctttat ctctactatt ctttatctct atttatttat tacaactccc taacatttac
     5101 cactactgcc ttaggtctag tcatagcctc gattgaatac ttaacacctt gagttcccat
     5161 accagatgcc tttacaccta aaaatgggaa gtggtctgga cctctttcag tcttattatt
     5221 tatttgaaca gtaccaactt caagtttatc agctatataa aatgccttat caatatcact
     5281 tgtaaatact gatgattgaa gcccatactc tgattgatta gcaatttcaa ttgcttgatt
     5341 tatatcttta actcttataa ttggaagtac tggaccaaat ggttcttccc aagcaatttt
     5401 catatcaaca tttacattat ctaacaatgt tggatagaat aaattatttt ttctaacatt
     5461 gcctgttatt aactttgctc ctttatgtaa tgcatcatca accaaccctt gaacaaaatc
     5521 agttgcttta ttatctataa gaggagtgat aactacttca tccattggat ttccaattgt
     5581 caacttctct accaatggct ttatttttcc gactaattta tccgccacac tctcttgtac
     5641 taaaattctc tttactgccg tacatctttg tcctgaatat gaatacgctc cagatactat
     5701 attcttagct gcaaaatcta agtctgcatc ttctaatact atagccgcat cttttccacc
     5761 aagctccaat aataatggta ccattccaga tattttagat atatgctgac caacttcagt
     5821 actaccagta aagttaataa aattaattcc tttatgcgtt acaacataat ctcctatctc
     5881 actaccccta cctgtaatag tattaagtac tccatctggt atcccagctt ttctcataat
     5941 ttctaccaca tgaagtgcgc taatagcccc ttgtgtagca ggttttaata caactgcatt
     6001 tccaccaata agtgctggag caatctttga cattgataaa tttacaggat aattaaaagg
     6061 agatatagct aaaacagtac ctaatggaac ccttgaaaca taagacatct tatttcttgt
     6121 tccaccaggg aaattatctc cactaatggc ttcaccctcc atattttttc ccacatcagc
     6181 tgtgtatctt aaaaaatctg ctgttctctc aacttctgcc cttgcagatt ttatgtcttt
     6241 ggctatttca agtaccagta tattagctat ctcatctata ttttctaaca ataaatcagc
     6301 agccttatga aatatattgg ctctcttata tacaggcatt tcagcccata cttttatact
     6361 ctcttttgtg ttttttataa cctcatctac ctcatgtttt gtcatagatt gtactttccc
     6421 aacaagagaa ttatctattg gtgagtaaat ttctattggc tttctcgaat tagattctac
     6481 ccattttcca tttattaaat ttttatatac attttctttt gttttaagtt cattaaacat
     6541 atttttattc ctctctctga tataactttg tgtatttaaa ttatagccat ttattcgtta
     6601 tgtaaacatt tctttttttc ataaacgttt acctataaaa aaattttaag ttaacgtttt
     6661 tctacattct tctattttaa taacatactc gctccaaatc tatataggtc acactttaat
     6721 atcaatatac taatatacca atataccaat ataccaatat accaatatac caatatacca
     6781 atataaatat tataaacata aatcacttga caaatttata agtagacaat attatatatc
     6841 tatgacttaa aatattttta aagtcataga tatcatatat agatagaaag gattgataaa
     6901 aatggctaaa agttttacag aaatagaaaa agacaatata agaaaaagac taatttcaga
     6961 atgtgaaatc aactggtcta aatatggata caaaaaaact aatattgatg aactttgttc
     7021 aaaatcagga attgcaaagg gttcattcta cacattcttt gattcaaaag aaaagttatt
     7081 ttttgaagta ttaaatggaa tccaaaacag acttatatca tcagtagatg aaattttaag
     7141 taacatgcct ccaaaagaag gctttgcaaa agccttaaag atgttgtatc gattatatga
     7201 taaaaaccca ttcttatatt caccaaataa tactgattac atcactttat tgaataagat
     7261 acctaaagag atgctttcac aacttgaata cagcaatgta caaagttttt atagcttaat
     7321 tgaaagacat aatctcaagt taaaaataga gaaagaaaaa gcctttggtg tttgcaatgc
     7381 cctactgtca agcctattgc taaaagaaga tattggatat gactacattg aagtatttga
     7441 ttttatggta gacaatcttg tagaacacat acttgagtaa taatttaaaa taaattttct
     7501 aaacatttct ttataactag gaggagtaat atgattgaat taatacaaag actaaatgat
     7561 attatttaca aaataaatga taaactaaat atccacacaa aacttgtaga ccctagcagt
     7621 gagctcatgc cactttactc tatgttatct ataatcgttt ctatatttct aataatatta
     7681 ggtattaaag taagaaaaaa attccttata ataattgcta ttatatcaat tattataaat
     7741 attttaataa tcttagatag gtaggtacat atatggtaaa atcctcgaac acttttaata
     7801 aaaacttcaa cttgatgata ttaggtcaaa taatctcatt gtttggtgca tcaatactta
     7861 aatttgcttt atctttatac atacttgata taactggtaa agcagaaata tttgctacta
     7921 tactagcagt atcttcaatc ccaattatag tattctcccc tattggtgga gctattgcag
     7981 atatattcaa cagaagaaac ttaatggtta tttttgattt ttcaagtagt cttacagtac
     8041 taatcttagc agtattctta ttcaacaata atggttcaat attacttgta ggaattatta
     8101 tgaccatatt atcagtcata agcacaatgt atcaaccaac tgttcagtca agtacacccc
     8161 ttttagttga taatgagcat ttaatgaatg caaatggtat tgtagctgga gttgcatctc
     8221 ttacaaatat tgcaggacct gtattaggag gtgtcttgta tggtgtgatt ggaattaatg
     8281 caattatagt tataagttgt gtaagtttct ttctatctgc aattatggag atatttatac
     8341 agattccttt tatcaaacaa gagcaaaaca gcaacattat aaaaactata tttactgata
     8401 taaaggatgg tattgttcat atatgtaaag aaaatagatt tatactaaaa atactttttt
     8461 tagctatagc aataaactta tttttaagct ctatgttaat agttggtatc ccctatatag
     8521 taaaaatcac tatgggagct agtaacttct tatatggtct agcagaagga gcaatttctt
     8581 ttagttcact ggtagctgct gttagtattg gtattttctc aaaacatcta aaaatcacta
     8641 atttgcatat ttcctttatt atatgtgcag ttatgttcat accaatggcg gtttcagtat
     8701 atccatttat attaaacaca ggatttccag ttccattttt aatctttata gtatcatcta
     8761 tgctaatatt ttttacaacg actcttatct caatatatgt tataacagtc acacaattaa
     8821 aaactccaaa tgaactctta ggaaaagtaa tgtcaattct ttttgcagga acagcaattg
     8881 caatgcccct tggtcaggtt atgtatggta aactttttga tttattatca gatagagtgt
     8941 acatcattat actaggagtt ggatttatta cattacttat agcatttgta tcaaagataa
     9001 cattgaggaa cgaaaatctt tagatacaaa gaatttagat ataaagattt aattttcact
     9061 aatttctgaa tttaaatgca aaattgtgta tcagtatgtt gacgttttag aaattgattg
     9121 atataatcaa tggaaaataa actttataaa aattcaaaat attcagctag aggagtgata
     9181 aaaatgagtt acatgccaac tttagaacaa gcatgggaaa tattaagaaa atacaataag
     9241 gaagaatttc atataagaca tgctcaaatt gtatctggag ttatgagata ctatgcaaag
     9301 gagtacgacc ctgaaagagt agaattttgg gaaatagttg gacttttaca tgatttagat
     9361 tttgaaatgt atcctgatga acattgtgtt aaacaaagag aattaatgac agagcttgat
     9421 ttagataaat caataatcga ttctacaata agtcatggat atggattaac tggttcagat
     9481 gttaaaccag agcaatttat ggagaaagta ttatttgctg tggatgaact tactggttta
     9541 attggtgctg ctgcaataat gcgtccttca aagagcgttt ctgatttaga attaaaatct
     9601 gttaagaaga aatttaaaga taaatcattt gctgctggct gttctcgaga cgttataaga
     9661 agtggtgcag aaatgcttgg atgggaactt gataagctta tccaaacaac tatagaagct
     9721 atgagaagct tgattccaga gatggcaata tagctcctcc atacatatac catagaagtt
     9781 acatctttaa agagcatttc tctttttgat gcaacttctt tttatatttt gtagaataaa
     9841 aagtttaata ttttaaactc actatataat
//
LOCUS       DACULQ010000140         9858 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.141, whole genome shotgun sequence.
ACCESSION   DACULQ010000140 DACULQ010000000
VERSION     DACULQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.141"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            88..1128
                     /locus_tag="JA154_14155"
     CDS             88..1128
                     /locus_tag="JA154_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7996989.1"
                     /translation="MNLSKKIKKLFLDDKGYYFNSNWKDIHIGIYNTKLGDSLKYITN
                     EYIENLERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYICLEAIKKDCNALKYVRNKTERLIIKVIRHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVENQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            1267..1719
                     /locus_tag="JA154_14160"
     CDS             1267..1719
                     /locus_tag="JA154_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAT7996990.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDDGREMILTPKGEFYKEIVEELIK"
     gene            1719..2435
                     /locus_tag="JA154_14165"
     CDS             1719..2435
                     /locus_tag="JA154_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAT7996991.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2425..2901
                     /locus_tag="JA154_14170"
     CDS             2425..2901
                     /locus_tag="JA154_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-protein-alanine N-acetyltransferase"
                     /protein_id="HAT7996992.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2907..3923
                     /locus_tag="JA154_14175"
     CDS             2907..3923
                     /locus_tag="JA154_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAT7996993.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKKGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKEKGYNIITLSGGVAS
                     NSGLRAKITELAKDSGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4220..6928
                     /locus_tag="JA154_14180"
     CDS             4220..6928
                     /locus_tag="JA154_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7996994.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKSAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATADGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6932..7189
                     /locus_tag="JA154_14185"
     CDS             6932..7189
                     /locus_tag="JA154_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7996995.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7182..8132
                     /locus_tag="JA154_14190"
     CDS             7182..8132
                     /locus_tag="JA154_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAT7996996.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLGELQDIFLDNGIACYVGHETAF"
     gene            8447..9409
                     /locus_tag="JA154_14195"
                     /pseudo
     CDS             8447..9409
                     /locus_tag="JA154_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086625.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(9508..>9858)
                     /locus_tag="JA154_14200"
     CDS             complement(9508..>9858)
                     /locus_tag="JA154_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT7996997.1"
                     /translation="FNKFGKKLGVIILGICWSLWHLPLIFTLYTPETPILGLILRSIH
                     IVGISIFLGYLYIKTKNIWFCAIIHILNNSAFLITNSKESTITYHDILIVSMVVLIFY
                     VPFLFTKEYKNNLE"
BASE COUNT         3860 a         1018 c         1845 g         3135 t
ORIGIN      
        1 aaaataaagt tattattaaa aaaatataaa gattagtaat caaattttta ataataacta
       61 atttaataat gatgggagtg tatattcatg aacttatcta aaaagataaa aaaattattt
      121 ttagatgata aagggtatta ttttaatagt aattggaaag atattcatat tggtatttat
      181 aatacaaaat taggagattc tttaaagtat atcactaatg aatatattga aaatctggaa
      241 agggtaaaat atgactatcg tgaattaaaa aatatagaaa atcaaactga tggtatgtgt
      301 attttagcaa taaggcagaa ttacaaggca ttagagtatg taaaaaatca aaccgaagaa
      361 ttatgtttag aagcaataaa gtataattat aaagcacttg aatatgtaaa agagcaaaca
      421 gaatatatat gtcttgaggc tataaaaaag gattgtaatg cacttaagta tgtacgaaat
      481 aaaacagaaa ggcttattat aaaagtaata cgtcattctt ccaatataga tgttgtaagt
      541 atactaaagg ctttagagac tcaaacaaga agaatatgtt tagaagcaat taaaaaagat
      601 ggcaggtgtc ttgcatacgt tagagaacag agtgaagaat tatgtataga ggccattaaa
      661 caaaattata aagcattaaa atatgtagaa aatcaaacgg aaaaaatgtg tatagaatcc
      721 gtaagacaaa atggtatggc attacaatat gtaaataaac aaactgataa aatatgtata
      781 gaagctgtca aacaagatgg aagaagtcta caatttgtca ataataagac agaagaaata
      841 tgtataaatg ctataagata tttaaataaa aaatataata ttaaagatgt actaagttac
      901 atagataagt atacagaaga tatatgtata gagattgtaa gacaaaatgg aaaaatgctt
      961 atgtatataa aaaatcaaac agaaaaaatg tgtattgaag cagtaaaaga gaactataag
     1021 tctcttaagt atgtaaaaga gcaatctgag agaatatgta aagaagcatt gaagcaaaac
     1081 cataaagcaa aagagtatgt aaaaattgct attgatgatt gtatataagg tgtagataca
     1141 gccatcaata gtgaaagata aaaaaagata aagacaagta ctttataata atctaggctt
     1201 atactataat taattaagca gtgatataaa ttcacatgac gattatatca aaatggagat
     1261 gaattgatgg caaaaatata tttagaaaat gaaaataaaa ctagagaaat aggatataaa
     1321 ttaggtaaac ttttaaaaga aggcagtgtt atctgtttag taggagattt aggagctggt
     1381 aaaactacaa tgacacaaag cttggcagat tcattaggga tagaagatta tataacaagt
     1441 cctactttta ctataataaa tgaatatgaa ggaaaaatac ctttgtatca ttttgatgtt
     1501 tatagaatag gtagtagtga cgaaatgtat gatattggtt atgatgaata tgtaaattca
     1561 aatggaatct gtataataga atgggctaat ctaatagaag atatacttcc aaaagaatat
     1621 ttgaatatag aattgagata taaagatgac ggaagagaaa tgatattaac tccaaaagga
     1681 gagttttata aagagatagt tgaggagttg attaaataat gaaaatacta ggtatggata
     1741 cgtcttctat ggcagcaagt gtagcagttg ttgaagatga taacttaata tgtgagttta
     1801 cagtaaacaa caaaaaaact cattcacaaa agcttatgcc aatgatagaa aatatgttga
     1861 gtatgagtga tttaagtata aaagatatgg atttattagc tgtatgtata ggtccaggct
     1921 cgtttactgg acttagaata ggtatggcca cagtaaaagc tatggctcat gtaaataata
     1981 taccaataat agcagtaaat tctttggaat ctctagcaaa taacataaat ttctgtgata
     2041 gaaagatatg ttgtatatta gatgcgcaga aaaatcaagt ttattcagct aaatacaagt
     2101 ttgaaaatgg agagatgata gaattagatg gtgtagatgt tgtagagttt gatgctttag
     2161 tagatgaaat tgtatcaact aatgaagaat ttataatagt tggagaagct gtatacaaat
     2221 ataaagataa gttagagaat ataaaaaata taaaaatacc ttcaccagct aataatgtat
     2281 ctaacgcagg aagcttatgc tctttagcat taaataaata taataaaaat atagatgtac
     2341 atacttgtta tacgataaat cctatgtata tcagaaaatc tcaagctgaa gtacaatatg
     2401 atgaaaaaat gaagaggcta aataatggaa gataatacaa aactaatagt agatgatata
     2461 aaaatagaag aaatgactac agaagatata gatgaagttt ttgaagtaga aaaaaattgt
     2521 tttgaagatt attggtcaaa agagtctttt agaaaagagt tgtctaatga agtagcaagg
     2581 tatatagtag ctaagttaaa tggaaaaatt gtcggatatg tgggaatttg gcttatactt
     2641 gatgaagggc atataaataa tgtggctgtt catagtgact atagagggaa aaaaataggg
     2701 gacaagttga taaaaggcat agttgattta tgtaaagata ataatattgc atctatgaca
     2761 ttagaagtta gagcatcaaa taaaatagca caaaatttat atagaaaata tggatttaaa
     2821 atgggtggaa taagaaaaga atactacagt gataataaag aagatgcaat tataatgtgg
     2881 aaccaattaa aagaggtgta gagtttatga gtgatataat aacattagct atagagagta
     2941 gttgtgacga aacagctgca tcagttttaa aaaatggtag agaagtactt tcaaacataa
     3001 tctctactca aatagagaca cataaaaaat ttggtggtgt agttccagaa gtggcttcaa
     3061 gaaaacatgt ggaaaatatt gatatagttg ttcaagaagc tttagacaaa gcaaatatag
     3121 gttttaatga tatagaccat atcgcagtta catatggacc tggactagta ggtgctttat
     3181 tggttggtct atcttatgct aaagcacttg catatacttt gaatattcca cttgtaggag
     3241 taaatcatat agaagggcat ctaagtgcaa actatataga gcataaggat ttgaaacctc
     3301 cttttataac attaatagta tctggaggtc atactcactt ggttgaagta aaagattatg
     3361 gcaagtatga aattttagga aaaacaagag atgatgcatc tggagaagca ttcgataaaa
     3421 tttcaagagc aatgaattta ggttatccag gaggacctat tattgataat ctagccaaga
     3481 agggaaataa acatgctata gagtttccaa gagcctattt agaagaagat agttatgatt
     3541 ttagttttag tggattaaaa tcttctgtat tgaattatct aaatgggaaa agaatgaaga
     3601 atgaagaaat agtagttgaa gatgtagcag catcttttca agaggctgtg gtagaggttt
     3661 tatctactaa ggcactaaaa gcagtaaaag aaaaaggata caatatcata actctttctg
     3721 gtggagttgc ttctaattcg gggcttagag ctaaaataac agagcttgct aaagacagtg
     3781 gtattactgt taagtatcct cctctaattt tatgcacaga taatgcagct atgatagggt
     3841 gtgcaggata ttataatttt ataaacggga aaacgcatga tatgagttta aatgcagttc
     3901 caaatttaaa gataaatcag tagagttaga taaatttgta aatataaaag tattgtttca
     3961 aaaaagtaag cagaagtgaa attaaatata ataaaaatca atatagtata tattaaaact
     4021 tgaagtaaaa ctatttataa tctttatatt ccaatgaatt gcaacacatt ttaggatata
     4081 caggttgtct ttggcttaga tttgcttcaa gtttaatttt ttctatagaa agattatttt
     4141 aaaaagtggg taatataccc ttttttttca ttcttaaaaa gttttatact aattatagaa
     4201 agattaagga ggggaagaaa tgagtcaaag taaagaagac aaaataagaa gtattttgga
     4261 agcaaaaaat atcaaatcaa attttcaaaa taaagaaaat ttaagtgaat ttaatgaaaa
     4321 aaaagcaagt aaaagagcag aagacttatt agatgtatat tataatacat tatctactgc
     4381 agatatggaa tttccatatt ggtataatag agaatataga aaatcagatg gtgatatacc
     4441 agtagttaga agagctaaag ctcttaaatc tgctttttca catatgacac caaatataat
     4501 acctggtgag aaaatagtaa tgcaaaagac acgccattat agaggttctt ttccaatgcc
     4561 atgggtaagt gaaagtttct ttgttgctca aggagaacaa atgagagaag aggcaaagaa
     4621 attagccagt aacacagctg atgaacttac aaaatttggg tctggcggag gaaatgttac
     4681 tgaaagtttt ggaaatgtcg tttcaatagc tggtaaattc ggaatgagaa aagaagaagt
     4741 acctgtactt gtaaaaatgg caaaagaatg ggttggaaag tctgttgaag atttaggttt
     4801 tcactatgaa aaaatgatgc cagattatga tttaaaagaa aacctaatga gtacacttat
     4861 atgtatgttt gattcaggat atacacttcc tcaaggtaga gaagttataa attattttta
     4921 tcctttaaat tatggattag atggaattat agaaatggca aaagaatgta aaaaggcagt
     4981 tgcaggaaat gcatctggag atgggcttat tggtatggac cgtctatatt tttatgaggc
     5041 tgttattcaa gttattgaag gattacaaac ttggatttta aattatgcaa aacatgcaaa
     5101 atatttagaa agtatagaga cagatttaga agctaaaaaa gaatactcag atttagtgga
     5161 gatattagaa catattgcac ataaacaacc tcgtacattt agggaagctc ttcagttgac
     5221 ttatacaatt catatagcat cagtaaatga ggatgctata tctggtatgt ctataggccg
     5281 ttttggtcaa attttatatc cttggtatga acaagatata gaaaaaggat taattacaaa
     5341 agaagaggtt atcgaattat tagagcttta tagaataaaa atcacttgta ttgactgttt
     5401 tgcttcagcg ggcgtaaatg gaggagtact atcaggaaat acatttaata ctttatctat
     5461 aggtggatta aaagaagatg gttctacagg agctaatgaa ctagaggagt tattattaga
     5521 agcaagtatg agatgtagaa ctcctcaacc tagtttaact atgttatatg atgaaaaact
     5581 tccagaagat ttcttaatga aagctgctga atgtacaaag cttggttctg gttatccagc
     5641 ctgggtaaac aactcaaatg gaacaacatt tatgatgaaa caatttgctg atgaaggtat
     5701 gactgttgaa gaagcgagag catttgcatt aggaggatgt ttggaaacgt ctccagggtg
     5761 ctggaaacag ttaactttaa acgggaagac atactctatt gcaggtggag caggtcaatc
     5821 tgctggctct ggagttcact ttatagctaa tcccaaaatt ttagaacttg ttcttatgaa
     5881 tggtaaagac catcgtatga atatacaagt atttgagcct cataataagc cattagatac
     5941 ttatgaagaa gtcatagaag tatttaaaga ttattataaa caagcaataa atgttttaga
     6001 aagagcaaat aacattgagt tagatatatg gagaaaattt gatacttcta taataaattc
     6061 actattaaag ccagactgtt tagacaaagg tcaacatata ggaaatatgg gatatcgtta
     6121 taatgcaaca ttaaacgtag aaacttgtgg tactgttact atggtcaatt catttgcagc
     6181 acttaagaaa cttgtatatg atgacaaagc atttacaata gaggaaatga aggatgccat
     6241 attaaataat tttggattta aagatgcatt agaagtagga aattattcta tggcagacca
     6301 agtaaaagta gataagactg gtaaatatga tgctatttat aaggcctgtc ttgatgcacc
     6361 taaatatgga aataatgatt tgtatgcaga taatattctt aaaaactatg aggtatggtt
     6421 atcaaaagta tgtgaagaag cacaatcttt atatgctaag aagatgtacc cttgccaaat
     6481 atctgtttct acacatggac cacagggagc tgctaccttg gctactgcag atggtagact
     6541 aagtggaaca acatattcag atggttcagt ctctgcatat gcaggaacag ataaaaatgg
     6601 ggtatatgca ttatttgaat ctgcaactat ttgggaccaa gcagtagttc aaaattctca
     6661 gatgaattta aaacttcatc caacaactat aaaagggcaa caaggaacta aaaaattatt
     6721 agatttaaca agaagttatt taagaaaagg tgggttccat attcaatata atgttgtaga
     6781 ttcagaaaca ttaaaagatg cacaaaaaaa tcctgacaat tatcgtcaat taatggtacg
     6841 tgttgctggg tttactcaat attggtgtga attaggtaaa ccaatacaag atgaagtaat
     6901 tgctagaaca gagtatgaag gggtgtaaaa tatgagaaag catagtgatt gtatgaattt
     6961 ttgtgcagta gatgcaacca aaggaatttg tagattatca aaacaaatga ttaatttaga
     7021 tgatgcagca tgtccagaga taaaagtaat gccaaaatgt aaaaattgta aaaattttgt
     7081 tgaagctaat gatgaaggta taggcaaatg tgttggccta gagaaagaag actgggtata
     7141 ttcaacattg aatgcaatta cttgtgaagg acatgtgttt aatgagtagt caaaagcaat
     7201 tagaaggcat gattttcgat gtacaaagct tttcagttca tgatggtcca ggttgtagaa
     7261 caactgtatt tttaaatgga tgtccactga gttgtaaatg gtgtgcaaat ccagaaagtt
     7321 ggactgtaag accacatatg atgtttagtg aattatcttg tcaatatgaa aatggatgta
     7381 ctgtatgtca tggtaagtgt aaaaatggtg cattgagctt taatcttgat aataagccag
     7441 ttatagattg gaacatctgt aaggactgtg aaagttttga atgcgtcaac tcatgttatt
     7501 ataatgcgtt taaattatgt gcaaaacctt acactgtaga tgaacttgtg caggtaatta
     7561 aacgtgattc taacaattgg agaagtaatg gaggagtaac atttagtgga ggtgagccac
     7621 tacttcagca tgaattttta catgaagtat tattaaagtg tcatgaggta aatatacaca
     7681 ctgccattga aacgagtgca tgtgtatcaa atgaagtttt taataaaata tttaacgata
     7741 ttgattttgc atttattgat ataaagcata tggatagaga aaaacacaaa gaacaaacag
     7801 gtgtgtataa tgatttaata ttagaaaata tatcaaacct tgcaaattct gattggaatg
     7861 gaagattagt acttagagta ccagttatct ctgggtttaa tgatagtgat gaaaatataa
     7921 gtgatattat atcttttatg cacaaaaata atttggtaga aattaatcta ttaccatttc
     7981 acagattagg agaatcaaaa tggattcaat taggcaagga atatgagtat tctgataagg
     8041 gtgatgttga tgaaggtcat cttggagaat tacaagatat atttttggat aatggtatag
     8101 cttgttatgt tggtcatgag acagctttct aaaattaaat acaagtttta attaaaaaag
     8161 gatgttacac taaaagctag agttacatcc ttttttatgt ggtttttatg tagaaataat
     8221 taagtaaagt tttatatatt ttatatattg tttatattgc tttatgtcca taaatattgc
     8281 tttaaatata ctttatatat tttttatagt ttaaattaca gttagagaat atattacgtt
     8341 tactttattg aagcagaagt tttgtggtaa aatactacta tgaaaaaatt taatagaata
     8401 tattttagaa acaactaaaa ataatagtta ggaggaagac atctatatga acaaatcatg
     8461 gagcaacttg attattaaga ataaagacaa aaaagtacgt tcagggtgga ttattatagc
     8521 tgttatggct accttttaca tatttcagta ttatttaagc atgatacttg tggaaataat
     8581 aagaaaaatc ctggttgcta caggggacat taatttaaaa acaaactatt tttcagaata
     8641 tgctaattgg ttaaatgatg ttgggttaca gattgtattt caaattctaa cagattcact
     8701 tatgattatt attcctataa ttgtatggag atttattatg aaaaatccta ttagtgaaat
     8761 gggattacat tcttatagaa ttaagaaaaa agaaggatat gtaggaatgt tattaggaat
     8821 atttaattgt acagtaatct ttattataac aataacaatt ggtggtggtc atgttacttc
     8881 atgggtacca aaaatttcac ctttaacagt ttcttgaata cttgtcttta ttttagttgc
     8941 ttttgcagaa gaaattttaa atcgtggttt ttttatggca gttttacgaa gatgtaaaaa
     9001 tatttatttt ataatgattg ttccatctgt tatttttggg ttgattcata tatggaatcc
     9061 tgatgttact tttttgtctg taattaatat tataattatt gggattcttt tttcatatat
     9121 gtttataaag tcgtcgaata tatggatgtg tataggttat cattttacat ggaatgtatt
     9181 tcaggggatt atatatggta tgcctgtaag tggacttcag gttccaggga ttataactac
     9241 acaaatcact catggtaatc tattaaatgg tggtatgttt ggtattgaag gtggaatttt
     9301 gactactttt gttaatctac ttagttttat atttgtatgg tattactata gagattctaa
     9361 gtatgatttt gttagtgata aagtaaatgt gaccaatatc aagaattagt tgattataat
     9421 aaattttaat aatatcttct aagcttaaag tattctgtag gattgttgga aatttacaaa
     9481 aatataaaaa taacttggat attctattta ctccaagtta tttttatatt cttttgtaaa
     9541 tagaaatgga acataaaaaa ttaaaacaac catagatact attagtatat catggtaagt
     9601 gatagttgat tctttagaat ttgtgataag aaaagcacta ttatttaaaa tatgaattat
     9661 agcacaaaac cagatgttct tagtttttat ataaagatat cctaaaaata ttgaaattcc
     9721 aactatatgt atcgaacgta atattaatcc taatattggt gtttcaggtg tatataatgt
     9781 aaatattagt ggtaagtgcc acaaactcca acaaatacct aatattataa ctcctagttt
     9841 tttaccaaat ttattaaa
//
LOCUS       DACULQ010000141         9847 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.142, whole genome shotgun sequence.
ACCESSION   DACULQ010000141 DACULQ010000000
VERSION     DACULQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.142"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            80..997
                     /locus_tag="JA154_14205"
     CDS             80..997
                     /locus_tag="JA154_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HAT7996998.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            1075..1812
                     /locus_tag="JA154_14210"
     CDS             1075..1812
                     /locus_tag="JA154_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HAT7996999.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            1818..3668
                     /locus_tag="JA154_14215"
     CDS             1818..3668
                     /locus_tag="JA154_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HAT7997000.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNMIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFVKENKKIQSIYDYKLKSN
                     VLDLILKSKDLKHLINNTLRNIVKSNLESNNKSNISGLSCFNVVQLRTILNNYKRGVY
                     KMDYKKLRKIYESGCEIHDYYIDKNAKNKIEGITYKILNLIKVGNKNDFMQTILRIFS
                     PSEKTPPEEILQIFVEEDLDFESVGYAFVTGLISGKYEGKDKLPNEKEEK"
     gene            3670..4584
                     /locus_tag="JA154_14220"
     CDS             3670..4584
                     /locus_tag="JA154_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HAT7997001.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            4586..5287
                     /locus_tag="JA154_14225"
     CDS             4586..5287
                     /locus_tag="JA154_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HAT7997002.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            5298..7619
                     /locus_tag="JA154_14230"
     CDS             5298..7619
                     /locus_tag="JA154_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HAT7997003.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTYLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFANSDRNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            7667..8185
                     /locus_tag="JA154_14235"
     CDS             7667..8185
                     /locus_tag="JA154_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HAT7997004.1"
                     /translation="MPIDLEVLKVQGVKINYYYVCKRKLWLFSKGITMEQNSSRVLSG
                     KIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEMQLYYYLYYLKN
                     SMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSPPKIEKKHYCTK
                     CAYYEFCFVKEE"
     gene            8188..9168
                     /locus_tag="JA154_14240"
     CDS             8188..9168
                     /locus_tag="JA154_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtype I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HAT7997005.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            9171..9437
                     /locus_tag="JA154_14245"
     CDS             9171..9437
                     /locus_tag="JA154_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HAT7997006.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
BASE COUNT         4229 a          796 c         1690 g         3132 t
ORIGIN      
        1 tgtagaaata aataataaaa atatcaatta atgttattag ggggggttac tcattagtaa
       61 attagctaat acacttagaa tgatgatatt aattcaaagc agaggcaaaa tgaaatgcaa
      121 agaattatca gaagagttgg aagtaagtga aaggcaaata aaaagttaca agatgtattt
      181 agagcaagct ggtatattta taaattcaac acctgggatt tatggtggtt atgaaataga
      241 taaatgtaat agcatctcat taattaaatt attagatagt gaagtttcaa ttctagatat
      301 gataaattca cagttagagt ataataatga tatttacaaa aatgaattta ataatattgt
      361 agaaaaaata aaagcagtat taaatacagg agaaaaaagc gatacatata tggattattt
      421 tacagttcaa gcacaacgta attgtgatta tgagtctgaa aaaaataagt gtaatgaaat
      481 aattagagca tataccacaa aacacaaatt ttggatagaa tattattcat taaattcagg
      541 aaatagtgaa agaattgttc atccatatgg tttatttaat tataaatcag atacttatat
      601 ggtagctttt tgtgaaaaaa gatttaagtt tatagacttt aaattatgta ggattaaaga
      661 ttataaggtt ttagaagaaa aatataatgt agataaaagt tttagttggg atgaatatag
      721 taaaaatagt ataggaattt ataagggaga agaaattaat gttgttataa aaattagtca
      781 tccattttca acaattataa aggagaaagt ttgggttaat aatcaacaaa tcatagaata
      841 tgatgataaa tctataatgt ttaaagctaa aatgagaggt tatgaggaga taaaaagttg
      901 gattttaagt atgggagctt atgttgaggt tgtagaacca gatagactta gaaatgatat
      961 attgttagag attgaaaaaa tgaaaaaaat ttactgattt tttcaaataa tttatacttg
     1021 gaccaatata cttcacggtc tagtgttaat atattaattg agagaggtga tattatgaaa
     1081 atgaaaattg aatttagtac aggttgtata ccaatatctt ataattcgct ctttatgagt
     1141 attataaaag aagcaataag aaaatctaat gaagattact ataaaaacat gtactattat
     1201 aaagaaaaga ataataaaaa aactaaaaat tttacatttt cggtttatat aaaaaagtat
     1261 agtattgaag gtgacaattt tattatagaa gataaagtaa tattaaatat aagtactcca
     1321 gatttagaat tgggttttca tatatacaat gggttaatga cttctaaaaa atgtttatat
     1381 aaagactacg aacttacaag aattagaatc gatttatcaa gagaaaagaa ggtaacagaa
     1441 gaaagggtac tatttaatgc attgtctcca atctgtgtaa agtctaaaga aggaaaattc
     1501 ttggagataa ctgatgatag atacatagaa gaactaaact atataactaa tgaagtggtt
     1561 aaaaattata gggggaatgg attaaaaaga aagttagaat ttgaaaatat aggtctcaaa
     1621 aaggtagtag ttaaagagag tttaagagaa tttaaaaaaa taacaggtaa agagtatcaa
     1681 tatattaatg gttacaaagg taaatttata ttgaaaggtg atattgatga tttaaatctt
     1741 atctacaatc taggaattgg ttttagacgt gctcagggat ttggagatgt agatatttta
     1801 gaatggaggt gagataaatg aagttaagag tttatagagg agattggttt tttaatatgg
     1861 gtatagttgg atttttaaat ataataaaga aagcagataa acaagctgaa atatttatta
     1921 tggaagatta tatcgaattt gatagcttat ttcttgaaaa ctttcatgag tattatttta
     1981 attactttat ggatgaatat gatgtatcaa aaagaattaa aaaaaatatt gattacagta
     2041 taaattttat taaatctaag cctgatagaa tcaaagatgg aattaagaaa attaaagata
     2101 gtgtgaaaca acaaaatgat aagattaaaa aatttgatga agaaaaattt aacatgataa
     2161 aagagaagct agatagtatg tctaagataa aaagttatga tgaatttgat agtttagaag
     2221 ctttagtaga tgagactata gatatattta aaataaaatc aataaatgac aggcttacag
     2281 caaatcttta taaatatgta gttcaagata attattttgg acaagttagt ttttttaatg
     2341 ttgctaaggc gaaattagat ttagatggat taaaacaggt aatgtttaat gattatttaa
     2401 gacaaattat atattttgga gagttagaag atttattaga agagaatgac tatgacaagt
     2461 tgaaaaacta tttaaatgat agattaaata gtatagctaa ggatataaat gaaaaaaaag
     2521 ttagtaagtc tagtataaat actatagaaa aaattatgaa ggaaataaat aaaaacttta
     2581 taaagaaaaa taaaagcata gaagatataa aaaagtatat ggatagttta gaagtatgtg
     2641 aaatgtgtgg tttatataag ggaatattag atgaatactc agaaagtaat tttgctccac
     2701 ttggagtaag caccaataat gctaggaata tgttttggaa tcaggcgtat gtaccatcaa
     2761 tatgtgatat ttgtaagcta atattgttct gtactccagc aggggctact tatacaagaa
     2821 agaattattt aattaatgaa gaaaatgagt tttatttatt tgttaacatg gatacttgta
     2881 taaatgagct ttttgaaaga aataatagtt taaaagctca aaaaagtgag tattcagatt
     2941 ccaaagatga aaatcctttt aatcagctta taaaaagtat agtagaagaa aatacattaa
     3001 aaagtgaatg gcaacttcgt aatatacttt ttgtggaatt taaagcaagt atagattcta
     3061 aaaaatgtaa aataaattat tttaatattc caacttattt agcaaagttt tttgtaaaag
     3121 aaaataaaaa aatacaaagt atatatgatt ataaacttaa atcaaatgtg ttagatttaa
     3181 ttttaaaaag taaagactta aaacatttaa ttaataacac tttaaggaat atagttaaga
     3241 gtaatttaga aagcaataat aaatcaaaca tatcagggtt atcttgtttc aatgttgttc
     3301 aattaagaac aatattaaat aactataaga gaggagtgta taaaatggac tataaaaagt
     3361 tgagaaagat ttatgagtca ggttgtgaaa tacatgatta ttatattgat aagaatgcta
     3421 aaaataaaat agaaggaata acatataaaa ttttaaattt aattaaggtt ggtaataaaa
     3481 atgattttat gcaaacaatt ttaagaattt tttcaccaag tgaaaaaaca ccaccagaag
     3541 aaattttaca gatatttgtt gaagaagatt tagattttga atctgtaggg tatgcatttg
     3601 taacaggatt aatttctgga aaatatgaag gaaaagataa actaccaaac gaaaaggagg
     3661 aaaaataaga tgaaaagagg gttaacattt acagtaatag cacaggctca aagtttaaac
     3721 tatggggaag ggataggtaa tatatcagaa ttaaaaaaat taagtagaga tgatggaaat
     3781 atatatacat ttgcatctcg ccagtgttta agatatgaca tagtacgatt agcagcagaa
     3841 ttgtttaatt ggaatttaca ggttgtcgat aaagaaaaag gcactattca atttaaagac
     3901 aatataagta taagagaatc agaagaaatg gatttatttg gatatatgaa aacaaataaa
     3961 aaagatgaga aagataaaaa aggaggttct ttgactagag aagctactgt aagattaagt
     4021 aatgcaattt cccttgagcc atatagaagt gacatggact ttttaaataa taaaggatta
     4081 gcagatagaa taggagaaca tcctaatctt gctaacatag agcaacattt aagttattat
     4141 acatatacag tgactataga tttatctaaa ataggtaagg atggagatat agaacttgat
     4201 aataaagaga aatgtagaag agtagtagaa tttttagaaa taataaaagt attgaataga
     4261 aatattagag gtagacaaga aaatttatct cctctatttg tagtaggagg agtatatgaa
     4321 atagcaaatc catttttctt gggtagaatt aaattaaaag gagataaaaa tggatttaaa
     4381 ataaataagc aagctataga agaagtaatt caaggtactt ttttaggaaa agatttaaaa
     4441 gaatttacct atgttggaat ggtagatggg gtatttataa ataaagaaga atttaaggga
     4501 ttgtttgaag ataatttttt aagtgtagat aaattttttg gacaactagt taaagaagta
     4561 aaggaatatt atggggtgaa ttaatatgaa ggttttgaaa ttagatttat ttcaagaaac
     4621 tgcatgctac aaaaaaccat ttgctatgaa gattacagag acatatccac tgcctccata
     4681 ttctacaata aatggattaa ttcacaaaat attaaatgct acagaatata taccatttaa
     4741 tataagtgta cagggaacat atgaaagtat atttaataat taccagacaa catattttta
     4801 taaaaaagat agtattacat ctatgccaat gaatagtcat atgctattga atgtaaattt
     4861 gataattcat ataggtgggg attttgattt attggaagaa atatatgata gtttattaaa
     4921 ttatgacgag cacttatcat taggtagaaa agaagattta gtaagaatta atgatattag
     4981 atttgtagaa gtcaataaat ttgaagtaga tgaggatata caattagatg attatgaaaa
     5041 ttataagtta tcagaatgta atataagaat gcctatatat ataccaaaaa caagtttaca
     5101 agatacagat atagatggta taagttacag actcaataat tactatgata ataatgctgt
     5161 ggaagataaa aaaagggttt ggaataaggt agatagttat tatgttgagg atggaaatac
     5221 tatatcaatt ggagatatat tattagataa tgaaggagat ttattatatt ttaataatat
     5281 aaattaggag ggagattatg ttatatgcta aatctaatcc agtggaaaca cttagagaac
     5341 atactgatga acttttaaaa caaatgaatg tattaagaga aagttatgga aaaaatataa
     5401 atagcttaga ttttctagaa gaggagatat tttggcaatt acttgacttt gttatagaat
     5461 ttcatgatat tggaaaagca ttttcaccgt ttcaagagtt aataaaaagc agaatggata
     5521 ctaaaataaa tgaacctaaa atagtaactt atttagaaaa taatgtaggc cataattatc
     5581 tttctcctgc atttattgat tatagttata tagacaggaa aaaaaataaa gaacttagag
     5641 cagtattaaa tcaagttatt gtatatcatc atgaaagaga tatctttata gataaagatt
     5701 ttaaaaattt aatacaaaaa atattagatg aagatttaat aaataaagtt tatgaactac
     5761 agcatgaatt taaagttaga tatccaataa aaacagaaaa attgagtaaa gtatatctac
     5821 aaagtgtaga aaaaagaata gataaaaatc acaagtacta taatttatat attatgttaa
     5881 aaggaatact acatagacta gaccatagtg cttctgctca tgaagttgtg gaatgcaata
     5941 atgttataaa tataggagaa cagacagaaa attatttaat gaaagagttt ggtagtttga
     6001 gagaggctca atcatttgct aaatctaata gaaataagaa tataatactg attgcatcta
     6061 caggaatggg aaaaacagaa actgccttaa tttggataga taaagacaag gcatttttta
     6121 cacttccatt aagagtaagt ataaatgcat tatttgatag agcaaaaaat atagttggtg
     6181 ttggagaatc aaatgataca ttcttaggtt tgttacattc aacagcaata gattatctag
     6241 aagaaagtaa tcaagaaaat tcaagtgaga ttgtagattt agcaaagtta ttatcttgca
     6301 aactgacatt ttctacaata gaccaaatat ttaaatttcc ttttttgtat agagggtatg
     6361 aaaaagtgta ctcaacatta gcatattcaa aagtagtaat agatgagata caagcatatt
     6421 caccagaaat agcagctgta ttggtaaaag gtatagaaat gatacataag ataggtggca
     6481 gatttatgat aatgacagct actatgccaa ccatatatat agatgaatta aaaaaaagag
     6541 gtgtaatgaa tagtaactta gctgaattga catgtaatac agaaaaaata agacattgtg
     6601 tttctattgt agaaaattca atagatgaaa acttaggtaa aataatacaa agtggaatga
     6661 ataaaaaagt tcttgtaata gtaaatacag ttaaaagtgc tgtagaaaaa tatgagttaa
     6721 tagaagaaat agttaaacca aaaggaatag atgtcaactt aaatctttta cattctatgt
     6781 atatacaaga agatagagca aaattagaaa aatatataaa agaatttgcc aatagtgata
     6841 gaaatggaat ttggattaca acacaattag tagaggcatc tcttgatatt gattttgatg
     6901 agcttcatac agaaaattca acacttgaca gtctatttca acgatttggt agatgttata
     6961 gaagtcgtga gtatgaagaa aatagcccaa atatatttat atatacagaa aaagctacag
     7021 gaataggtag tatttatgat aaggatattg tagaaaaagg acttgagcta ctgaaaactt
     7081 ttataaatgg aaaagaatgt gtgaagatga aagaaaaata taaggtagaa atggttaaga
     7141 tactatactc taaagaaagt ctagaaggta cagcttttga aaagagattt accagtgctt
     7201 taaatatttt agatactata acgccatata gtttaggttc taaagatgca caaaatatac
     7261 ttagaaatat tgaaggatat acagtaattc cagaggatat atataataaa attgaagaaa
     7321 ctttaatcaa agattatgaa gagttgggac aagagcttat aaatgcatat agtaatgatg
     7381 atagacaatt gataaataaa ataaagtcta agagaaaaaa agcaagaaga gagataatta
     7441 aaaaaactgt aaatttacca atatataaag ctaaacaaaa tgtaattgac atacaggtga
     7501 aaggtcttga agatttaaaa atacttttat ataaatatga tatatacgaa aataagcata
     7561 caaaacagat atttggtaaa ggtgtgctta taagtaatga aatagaccaa tttatttgat
     7621 ttttatatta acaatgtggc taaaatttac taaagaaggt gattttgtgc caatagattt
     7681 agaagtatta aaagttcaag gtgtaaaaat aaattattat tatgtatgta aaagaaaact
     7741 atggctattt tctaaaggaa taaccatgga acaaaatagt agtagagtat tgtctgggaa
     7801 gatagtacat gaagattcat ataaaagaat gaaaaataaa gaaatattga ttgatgatat
     7861 cttgaagcta gatataataa aggatgagta tgtaaaagaa gtaaaaataa gttcaaagat
     7921 gaaagaagca gatgaaatgc agttatacta ttacttatac tatttaaaga atagtatggg
     7981 tatagagaaa aaaggtacta taaattatgt taaggaaaag aaacaagaag aattgatatt
     8041 gacagaagaa aaagaaagaa ttattgaaaa cacacttata aatataaatg aaataaatat
     8101 gcagttatct cctcccaaaa ttgagaaaaa acattattgt acaaaatgtg cttattatga
     8161 attttgtttt gtgaaggagg agtaacaatg tataaagact attatattat aagtaatggg
     8221 gatataaaaa ggaaagaaaa tacgatttac tattacaatt tagaaggtga gaaaaaaata
     8281 ttaccagtag aacaagttga tgatattcat atatatggag aagtagattt aaatacaaaa
     8341 cttataaatt acataagcaa atatggggtg atgcttcatt tctacaatta ctatggatat
     8401 tattcaggta catattatcc aaggaagaaa aatgtatcag gattcagttt agtatgtcaa
     8461 tcagcctgct atttagataa aaatgaaaga ttatacttag caaaatcctt tgtggaaagt
     8521 gcatcatacc atattttaag aaatatgaga tattacaaag ttgatgaaag tttaattgat
     8581 actataaagg atgaactaaa tgatttgaat gatgtcaaag aaatatcaga acttatgggt
     8641 aaagaaggca gaataagaaa aacatactat aaatcattta atcaaatatt aaaagatgat
     8701 tttgaatttt ataaaagaga aaaaagacct cccaaagacc cagtaaatgc tcttatgtct
     8761 tttggaaata gcataatgta tacgaatgtt ttaagtgaga tatataagac acaacttgac
     8821 cctacaataa gctttttaca tgaaccatca acaaagagat tttctttaag tttagatatt
     8881 gcagaaattt ttaagccaat tataattgac acaataattt ttagcctgat taataataaa
     8941 agaattaata agaatgattt tgaatatcaa gacaaaattt gttatttaag tgaaaaagga
     9001 aagaagaaat ttttgcaaga atttgaaaat aagatgcaga caactataaa acatagaaat
     9061 ctaaatagaa aagtttcata tagaatgttt ataaggttag agtgttataa attaattaaa
     9121 cattttataa atgatgaaag gtacaaagtt ttaaaggtgt ggtggtaagt atgtttgtta
     9181 ttgttactta tgatattgtt gaagcaaggt cgttaaatag aattagaagg atacttagaa
     9241 aatatttgac ttggacgcaa aattctgttt ttgaaggcaa tattactgaa ggaaagttac
     9301 ataaatgtat ttctgaaata gaaaatatta ttgataatag cgaggattca atctatgttt
     9361 atgagataaa aaatcctaat tcaattaaaa agaaatgtta tgggattgat aagtattctg
     9421 atgaaatgtt tatataggtt tgcagtgagc gatatttatg ctaaaatagg tgttaacagt
     9481 tggaatataa gggattgaag gtgtatgata attgttatca attgcactac tgctcgctca
     9541 ctgcaaattt tgatgttttt attgaattat aattgcttga ttgaagtgtt ttcaatgtat
     9601 tcaaatatac ctattttggg gttttatatt aactaagtgg tatgtaaata attttttcgg
     9661 atatttaata atttatttta aatttagttt tatattaact aagtggtatg taaatctcaa
     9721 cagtatctac aagaacttct atatcttcta agttttatat taactaagtg gtatgtaaat
     9781 taatttaggg taacttttat attattttct tctatcgttt tatattaact aagtggtatg
     9841 taaattt
//
LOCUS       DACULQ010000142         9832 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.143, whole genome shotgun sequence.
ACCESSION   DACULQ010000142 DACULQ010000000
VERSION     DACULQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.143"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            204..1634
                     /locus_tag="JA154_14250"
     CDS             204..1634
                     /locus_tag="JA154_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7997007.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1737..2840
                     /locus_tag="JA154_14255"
     CDS             1737..2840
                     /locus_tag="JA154_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAT7997008.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            2855..3091
                     /locus_tag="JA154_14260"
     CDS             2855..3091
                     /locus_tag="JA154_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT7997009.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3108..4157
                     /locus_tag="JA154_14265"
     CDS             3108..4157
                     /locus_tag="JA154_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HAT7997010.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVIEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALTSKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4159..4899
                     /locus_tag="JA154_14270"
     CDS             4159..4899
                     /locus_tag="JA154_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HAT7997011.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4902..5441
                     /locus_tag="JA154_14275"
     CDS             4902..5441
                     /locus_tag="JA154_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAT7997012.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5565..7664
                     /locus_tag="JA154_14280"
     CDS             5565..7664
                     /locus_tag="JA154_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAT7997013.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIENRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7931..8446)
                     /locus_tag="JA154_14285"
     CDS             complement(7931..8446)
                     /locus_tag="JA154_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HAT7997014.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8538..9752)
                     /locus_tag="JA154_14290"
     CDS             complement(8538..9752)
                     /locus_tag="JA154_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HAT7997015.1"
                     /translation="MMSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTK
                     MTLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVI
                     ISDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDI
                     NGTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYL
                     HIMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIK
                     RIESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLI
                     DCSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIV
                     SYNFFNGSINFY"
BASE COUNT         3683 a         1215 c         1826 g         3108 t
ORIGIN      
        1 agctttttag ttaattttag tagtaaaaaa taaaacttaa aaagtgtttg acaattatga
       61 attttgagaa tataattaaa ttaaaattat acacatataa aaatatactc ctgaatcaag
      121 aatgagatat ttaagattca aaatatatat tattatacgt gaataataaa aaagtaagaa
      181 aattttataa gggaggggta gatgtgagta gtcaagaaag caaaccaaaa ggaaatctta
      241 tatcaaattt tagagatgat atgagagacc ctaagaaaag agcaatatac acatattcaa
      301 gttctataaa cttagcgtac aattttatag gttactttgt atcaactgtt acaaatattt
      361 ttttaactga tgtggcaaaa cttggtgtaa ttatggcagg tggtatgcaa attatacaat
      421 ctttcataaa aattgctgta gctcctataa caggtacagt atttgacaaa caaccattca
      481 aaaaaggaaa gtattatcca tggcttagat atgcaccagc agcgttagca ttaacatatg
      541 gaatattttt catagttcca ttaatgaaaa taagtccagc attactttta cctttgatga
      601 cagctttatt attaatagca tcttgtgctc agcaagtagt tttcactata atttatgcta
      661 tttaccctgt agttgctaag acacctagag accgagttat aggctcaact actacgaata
      721 tatttaaaga agttggtaaa ttcttagtag gtttttcata tccgttatta ttagttttat
      781 ttgcagatgt attaggtgga gaaggaatgg gttatttagg tacctactta ttctttgcag
      841 caggatgcct tttagtattt tggttttctt caacagagat aataaaatct ggagcagaag
      901 aagaagttct tcaaaaagaa gagaagccaa agattaaagc aagtgaaatg gcaaaagctt
      961 tattcactaa tgtaccactt atgatggcat tttgtttaga atttcttata tgtataagat
     1021 ctataggatt aggacctttg gctccatatt attttaaata tgtaatagaa gatgaacgtg
     1081 gattagcagt atttttatca atcatgcctt tggtaagtgt agcattcatg tttttcgcac
     1141 cagtgtttat aaaaatctgt agagagcaaa aactagcttc aataatttct ttttctatat
     1201 gtgcactatg ccacttattg gttgcatgga ctccttgggg gaaaactact ttaggagtaa
     1261 ctatgttgct ggcaattggt ggaggttttt caaatgtagt atcaataata aatcttaatt
     1321 tctttgccgg ttcttgtgat tatgggcatt ggaaatcagg aaaagactta cctggcttaa
     1381 gtatgtcact atatccagtt gcaatacaag taggagtatt acttgcaact actataagaa
     1441 cagttttaat gaactcaatg ggatatcaag ctgatatggt agtcacagaa gcagttaaat
     1501 caggttttat aaatatgatt tcttattcta tggcaatacc tttaataata gctgttgtta
     1561 tagcaatact atatccagta agtgacaaga aattaaacca aataagagaa gagttaaatc
     1621 aaagaaatgc ttagttaaaa gaaagttaag tagatggtta atttaagcta ttaaagtaag
     1681 tagacgcaat agcgtttata taaatgaaaa tacatataca gagggggctt ttaaaaatga
     1741 tagaaaaatt attgagaaat accgtaaaag aattacatca atatgttcca ggtgaaccaa
     1801 tagaaaaagt taaggaaaaa tatggtgtaa aagaaataat aaaattagct tcaaatgaaa
     1861 atcctctagg accatcacca aaagcaatag aagctatgat tgaaatgtta aaacaaggtc
     1921 aactttatcc agagccagaa gctaacgaac ttagaagaaa attagctgaa aagctagatt
     1981 taaaaccaga aaattttatt gttgctaatg gtgcagataa cgttatcact ctaataggtg
     2041 aggcttttat aaatagagga gatgaagtta tatattgtaa cccaacattt ccatcatata
     2101 gaactgcaac tataaaaaat gaagggatac cagtagaagt acctttaaca gaagattata
     2161 agtatgattt acaaggtata ttagataaga ttacagataa aacaaaatta atatgtgtat
     2221 gtaaccctaa taatccaaca ggaacaattg tggatgataa agagttagaa gaatttttaa
     2281 agaaagtccc tgaaaatatt ataactatat tggatgaagc atatatagaa tttttaacag
     2341 ttcctaatta tgtggatgga cttaagtatg taagggaaaa ttataacgtt atagtaacaa
     2401 gaactttctc taaaatatat ggtcttgcag ggcttagagt aggatatggt attgctaaag
     2461 atgagataat aagaacttta tttacagtaa aagagccttt ctctgcaaat cgtgttgcta
     2521 taagtggagc aacagcagca cttgatgatg aagagtttat aaaagaaaca tatgaattaa
     2581 atagagttgg aatggcttac tttaaagaag agtttacaaa aatgggattt gatgttgtag
     2641 attcacaatc aaatttcctt tatgtagata tgaaaacaga tatacctaag ttatttgaag
     2701 acttgaaaaa aagagggttt gtaataagac caagtgctac acatgcacgt gtaagtattg
     2761 gaactatgga agaaaataag aaatttgtag cagttttaaa ggaaatatta aatattaaag
     2821 gagagccaag tattggataa tagatagaaa tattatgaaa cggggtgtag atatgaactt
     2881 aaaaataaac gcagagagat gtaaaagttg tgaatactgc gttatatctt gtaaaaaagg
     2941 agcattaaaa atatcaagta agataaacaa agaaggttat gctcatgtag aagttgatga
     3001 gagtaaatgc atactttgtg gaatttgcta tcaggtgtgt ccagataatg tttttgaaat
     3061 aattaaagaa gaagcatgtc aagaggctta gaaggagttg ggataatatg aaaaagatgt
     3121 ggaaggggaa ccacgccatt gcagaagcag ccctaagagg aggttgtgaa ttttatgcag
     3181 gatatccaat tacaccacaa acagaggtaa ttgaattttt atcacataga atgtctgaac
     3241 ttggaagaac ttttatacag tccgaaaatg agatggctgc tatatatatg gtatatggag
     3301 catatgcaag tggaatgagg tctatgacga gttcttcagg accaggaata tctttaaaac
     3361 aagaagggat ttcttactta tgtgcaaatc attacccttg tgtaatcgta aatgtacaga
     3421 gatgggggcc tggtcttggc tcattggact cagcacagac agactatcta agagatacta
     3481 gagggggagg aaatggagat tatcatttga tagtttatgc accaaacagt attcaagaga
     3541 ctgtagattt gatgtataac tcatttgatg tagcggagaa gtacagagtg cctgtcgaaa
     3601 ttttgacaga agcagcttta ggtcaaatga tggaaccagt agaatttcca gagtttaaga
     3661 agagagaagg agatttaggc tggacttatg atggaagtaa tagagaccat gcaaaggttg
     3721 cagataacca aaagccaact ttttgtatgg agaaacgtat gagaataact gaaaatgaac
     3781 aacaatggga agattatcaa gtagaagatg cagaatatgt gtttgtagcg ttcggtatac
     3841 catcaagaac aactatgaat gctgttagaa gacttagaga acaaggtgaa aaagttggaa
     3901 taataagacc aataacagta tggcctttcc catataaagc atttgaaaag gtaagtaaag
     3961 atgtaaaagg atttatctca gtagaatcaa cagacacagg tcaattagta gaagatgttg
     4021 ctttaacctc aaagaaagta tgtaaagaaa atgtacctgt gtatggttta tttagtggaa
     4081 atcatatacc aaaaacattg caagtaatgg acacttatag caaaataaaa tctggcgaaa
     4141 tcaaggaggt gttctaatat ggctacagaa aaattaacac ctagcgttgt ttcaatacca
     4201 aacaagtttt gcccaggttg tggtcatgga atagtaaatc gtataatagc agaggtaata
     4261 gaagaacatg gatatgaaaa aaatcatgta ttaacattag gagttggttg tgtctgcaat
     4321 atgaatttta gctggaatgg tgataagatg caaactgcac atggtagagc ttcatctaca
     4381 gctataggag ttaaagtagc acttccagat acattagtaa tgacttacca aggtgatgga
     4441 gatgcttatg ttataggtct ttctgaaact ttaaatacag catatagaaa tcataatgtg
     4501 actgtatttg taataaacaa taataatttt gcaatgactg gtggacaaat gtcatggacc
     4561 actatgccgg gtcaagtcac aacaacaagt gtaaatggaa gagatataaa gactactggt
     4621 agtccactga aagttcctga gatggtggca aacttctttg atgtagcata tgtagcaaga
     4681 ggttcagttc atagcccaaa ggaaataatt aatctaaaaa aatatataaa aaatgcaata
     4741 gaagctcaat taaatggaga gggatattcc ttagttgaaa tattagcacc ttgtccaact
     4801 aactggggtg tatcactaga gaaatctatc aaatggatgg aagaagaaat agtgccatat
     4861 tatgctcttg gcgaatttaa gcaaagggat ggtgaatagt tatggaaaaa gagctagtat
     4921 ttgcagggtt tggtggtcaa ggagttttaa ctcttggtct gattgtagct gagtcagcac
     4981 ttgaattggg gaaacaagta acttggatgc ctgcatatgg tccaactatg agaggtggaa
     5041 aagcctatag tgtagtaaag ttttctgatg agagtatagg tgggcctgat atggaagaaa
     5101 tagatgtttt agtagcaatg aataaaccat ctttagatta tataaattta gttaaagaag
     5161 gtggaacagt agttattaat acatctgcaa tagatgagaa tgtaacatta agagaagata
     5221 taagtgtagt taaaataaat tgtcaggaat tagctcaaaa agtaaacaat ccaaaagcag
     5281 caaatatagt agtacttgga gcattaattg ctaaaacagg gttatttgaa aaagaattag
     5341 ctttaaaaac tatgtgtgat ttctttgagg aaaaaggaaa aggaaagttt aatgttcaaa
     5401 atgaagcagc attcatggaa ggctataata acgccatttg ataaattacc cttatcttaa
     5461 gtgatttatc tcaagattta ataatcttta gtgccaagag aaacttcatc ttgagataaa
     5521 attcacttaa aatcacaact ataaacatat aggagcgtga atttatgagt ttggaaaaac
     5581 ttattagacc aaaaagtatt gctatagtag gagtgactga taaacttggt tttggaagaa
     5641 gtgcagcact tagtatagta aaaagtaagg aaacagatag agtatattat gttaatccca
     5701 aaagagaaga attgtttggg agaaaatgtt ataagacgat acaagaagtt ccagaagtag
     5761 tagactgtgt agtagtatgt acacctagaa atgtagttcc atctgtatta aaagattctg
     5821 gagagttagg aacaaaggca gcagtggtat atgctagtgg atttgcagag gaaggtacag
     5881 aggaaggtac agatttagaa aatcaactta tagaaatatc aaatacatac gatatgaaaa
     5941 tattgggtcc aaactgcatg gggttgctaa attgtataga taaggttaat ctatgggcag
     6001 gaggttctaa atgggattta gatactaaaa aacctggtat aggaattgta ggtcaaagtg
     6061 gatttataac tgctgaaatt gtttcaagtg attattttaa tatatcttat ggtttttcaa
     6121 caggaaatgg aaatatagtt acactagaag atgcagtaga tttccttgta gatgatgatt
     6181 atgcatcagt aatagcaatt taccttgaag gtttaaaaaa tcctcaaaaa tttatagatg
     6241 ctttaaaaag ggcagcacaa aaaagaaagc cagtaataat attgaaatca ggaaggtcag
     6301 aaaaaggtgc tatttctgct gcttctcata caggaaattt agctggttcg agtaaagctt
     6361 ttgaaagtat ttttgaaaaa tatggagtaa tttctgtgga aaatttagaa cagtttatgt
     6421 gtttagcaca agcttttagt gttttagatg gtaatttacc tgcaaattct aattttgcag
     6481 caataaactt ctcaggagga gaaaatacta tttgtgcaga tttagcagaa gaaaatggtg
     6541 tagaacttgc agaaatatct tctgaaacta aagaagaaat gaaaaaatat cttccaggat
     6601 ttgcaacacc taaaaatcca ctagatgcta caacagcatt attccatgaa aaggatatga
     6661 tagtaggttt attacatacc tttgaaaagg atgatagtgt tggatttact atggtaggtg
     6721 caaatataag agatgaagaa aatgaaatgc atgaaaccct ttgtaaagct gtaagtgaag
     6781 ctagagaaca aggattgaaa aaacctgtgt ttgcagttcc tacacttgaa ggaaatagat
     6841 atttagatta taggactaga ttagaagata atcaagtgcc tataatgtca tcagttggaa
     6901 ctacatttac ttgttttaat aaaatggcta agtttataga ttatgattat tcaaaaagaa
     6961 ctttagaatt taaagctgtt aagaaaagag agtcaaataa tgtagtagca ttatcagaat
     7021 tagatagtaa gattgagatg aaaaaatatg gagtaccagt tccaggacaa ggtaatgcaa
     7081 aaagtattga tgaattagat gaaatcttaa aatatattaa gtatcctgtt gttttaaaaa
     7141 taaattcttc tgaaatattg cacaaatctg atgttggagg agttaaaata ggaattgaga
     7201 atagagatga ggcagtagat gcatataatg agattttaac taatgtaaaa aaagcaaaac
     7261 cagatgctaa tattgatggt atattagttc aagaaatggt agaaagtggt atagaaatta
     7321 ttattggtat aactaatgat gaccaatttg gaccaatgtt attagtcggt ttaggaggag
     7381 tatttgtaga ggtatttaaa gatactacat tatatccatt acctataaat catgatgaag
     7441 ctattatgat gcttaaaaaa ctaaaatcat ttaagctttt aaatggctat agaggaagtg
     7501 agccttgtga tattgatgct cttgcagata tgatggtcaa gttaggaaaa tatgcttatg
     7561 aaaataagga tgaagtcaag gaaatagact taaatccagt gtttgtatat cctaaaggaa
     7621 aaggcgtatg tgcagtagat gccttaatag ttaaatataa atagtttata agttttggta
     7681 ttcaaccata attaaagcac cgactgaaaa tcacattttg taagttagat agtgtgattt
     7741 tcagtcaccc caaatttaat ttaaaaatat tttaaaatga aagtatagtt ttaagtttaa
     7801 ttgtgaaata gcactgtata aacaataaaa tactacgtat aaaacgaata ataaaaggtg
     7861 ttttaatgat aagaatcaat cttgattttc attaaaacac cttctttatt atattaaaaa
     7921 tttttaaacg ttatattgtc acaggtgggt ttgttggagc tgcatatgta cgattagcaa
     7981 attcttggtc aacaaagaat agaccaccct ctccagcaag tttaacacgt tttatattgt
     8041 cttcacaatt tgtttcttct tctgcttgtt cagatataaa ccaaagtaag aattgagaag
     8101 ttctaaagtc tgattcagag tttgctacag ctgctaagtt gtttattaaa gctgtaacct
     8161 ttttttcatg agctaacgta cgctcaagaa tatccattgc agaagtaaag tctgaatctg
     8221 gcattgggat agcatcaagc tgaacctttc ctcctacatt atgtaagtat ttgtaaaaaa
     8281 acatagcgtg gtctgtctct tctttatatt gaacataaaa ccaattagaa aatcctttaa
     8341 gtccttctgc ttcaaggtaa gaagacatag aaagataaag atacgcagaa tataattcat
     8401 ggttaatctg ctcattaagt aatttttcca ttttagctga aatcattttt atacctccaa
     8461 attttaaatg ctcttataaa atataataat attaaatgga aaaatattca ataaaagtgt
     8521 ttaaatattt tacatattta ataaaaattt atactaccat taaaaaaatt ataacttaca
     8581 atggctatag agccaattaa tggtgcattt gctccaaaat ttgaatgcct tacacttatt
     8641 tttttatatt tagaaaaact tgctgaagca gtcaattttt ttaataatgt attttctata
     8701 aatgtaccag gtatattaaa gtcatagcct attattatag aactacaatc aatcaatgta
     8761 agtgtattaa ctaatgcata tgatacgtat gtacaaaatt catcaagcaa aaacatggca
     8821 agactatctt tattatttgc ctcatctatt aacttgacta aagaaaaatc tttacagtta
     8881 attaatgggg agttaggata aatatggctc aatgattcaa ttcttttaat tagattactg
     8941 acacttgtat aataatctaa acatccatta ttaccacaac tgcacaatgg tccactaaaa
     9001 ttaattgatg tatgtcctat ttctccactt tgtcctaggt tgccagtatg aagtttattt
     9061 tctagaacta gaccagctcc aataccattc attatgtgta ggtatatata atttgatata
     9121 tttttaccta aaccatacat tttttctgct aatgcacccg aattagcatc atttatcaaa
     9181 aatattggca aatcagacat ttcttttata aaatctacta tatttaggtt tgatacattt
     9241 ccaaaatctg ggggatttag tatggtacca tttatatcat ctactggacc tattgaagaa
     9301 ataccaattc caattatatc tctcttgttg attttcatta ttttagtaaa cattcttttt
     9361 attatatctt ttaaaacatc atttgataga catttggggt aagtttcaga aagttgtttt
     9421 acaatattac cttttaaatc tgaaataatt accttacata tatttcttct tattaggatt
     9481 ccacaaatac aaggcgatat attagaaata tctaataaaa taggtttacg acctgatgaa
     9541 tgtgcattta aagttgatga ctctatttcc ctaatcatat tttcattaat aagttcagta
     9601 actagatttg ataaagtcat tttagtaagt cctgtagact tagctaaatc aactctggac
     9661 ataggtgtat ttgtagaaag taatttcaat attattaact tatttttaat ttttacatct
     9721 ttgatgttag aaccttgttt gttactcatc ataaatctcc ttaaataaag ttaagtgttt
     9781 tatttatttt caagtatact atcaccttat aatagagtaa cattttttta ct
//
LOCUS       DACULQ010000143         9596 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.144, whole genome shotgun sequence.
ACCESSION   DACULQ010000143 DACULQ010000000
VERSION     DACULQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.144"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            80..814
                     /locus_tag="JA154_14295"
     CDS             80..814
                     /locus_tag="JA154_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997016.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            1025..2206
                     /locus_tag="JA154_14300"
     CDS             1025..2206
                     /locus_tag="JA154_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HAT7997017.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKFSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            2229..2762
                     /locus_tag="JA154_14305"
     CDS             2229..2762
                     /locus_tag="JA154_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAT7997018.1"
                     /translation="MYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKHTCHDFD
                     MDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLIIIEGYK
                     NYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLNDYKNISKYIESCIK
                     NEILSISNVEIKNILKE"
     gene            2881..3156
                     /locus_tag="JA154_14310"
     CDS             2881..3156
                     /locus_tag="JA154_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAT7997019.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            3411..5111
                     /locus_tag="JA154_14315"
     CDS             3411..5111
                     /locus_tag="JA154_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997020.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            5474..6661
                     /locus_tag="JA154_14320"
     CDS             5474..6661
                     /locus_tag="JA154_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997021.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            6721..7884
                     /locus_tag="JA154_14325"
     CDS             6721..7884
                     /locus_tag="JA154_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7997022.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            8035..8838
                     /locus_tag="JA154_14330"
     CDS             8035..8838
                     /locus_tag="JA154_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAT7997023.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            8934..9494
                     /locus_tag="JA154_14335"
     CDS             8934..9494
                     /locus_tag="JA154_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HAT7997024.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
BASE COUNT         3732 a          955 c         1763 g         3146 t
ORIGIN      
        1 tataattatt gaaaaaagag ataactgtaa attgagatat agataaatac tataaaattt
       61 aagaaaggag gattgattta tgaagttaaa aaggtcatta gtttgtgtaa ctattttagg
      121 tattatactt gttggatgtc ataaagaaaa tactaaagag aaaaatcaag tagcaagtaa
      181 agcaacacag caaaaaacta tgacaaaagt tcaaaatgat gttaatgaaa ttatgaataa
      241 agattataag tacattataa agaatatggg tataccttat aatacatttt attatataaa
      301 acctaaagtt ttaaaagaat caaatacaat gcaagatata aatacatcaa gttatatgac
      361 attagtttat ccaaaatata ctggaaatga tgaactagat ggaagtgctt tatatgttga
      421 tattaacgaa aataaagttg tcaatgttga aaccaattct ttctcatcac aaggtatatc
      481 agtcattgat gctgaatcta gtatagtgat tgaaaaaagt gaccatgaaa aatcggctgt
      541 atcactagaa aattttagac atattgacct gggagagtat gttggtgttg aagattccag
      601 aataaatgaa attgtaggag atgcgaatta tgatttaaca gcatataatc atgaaggcag
      661 taaagtagtt aagagttata gattgaaaga agataacaaa atattaaaaa aagaggtact
      721 tacaataagt attgtggata acaaaataaa atctataaaa actattgaaa gtgataaaat
      781 tgtaaaaata ataaaaggaa ctttattaga ataaaatatt ctaaagcata tgatgctgtt
      841 tcaagataga aggaaatttc tagctgggaa cagttttttt attagattta taaaatttga
      901 actgcactta tagataacta ttagttataa ttaaatataa gataaaagta tttccaactt
      961 ttataatcta taatatatta catagaaata tcttaaataa aaaatatatt ggggagtatt
     1021 taaaatgata gaattagaag atgccataaa aattattgaa aaaaatgttt taccaataaa
     1081 cagggtcaag atagtaaatc ttatagatgc agtaaataag gtagttgcag aagatatata
     1141 ttcaacaatt gatagtcctc catttgatag gtcaccctat gatggttatg catataatgc
     1201 aaattctgaa aatagaaatt taaaggttat agggactttg tatgcaggag atgtatttaa
     1261 tagggtatta aataaaaatg aagcagtgaa gataatgact ggtggaaaga taccacaggg
     1321 tgcaaattgt gtaataaaaa aagaagatgt aacagttata ggtgatatga taactttaaa
     1381 tataaaatta aaggaatacg ataattacat atttaagggt gaggatataa aaaaggggca
     1441 attgattatt aataaaaatc agaatatagg atatgatggt attggagtat tagcaagtct
     1501 tggaatatcc aaagtaactg tttatgatga tttaaaagtt ggaattttga atacaggtac
     1561 agaactacaa gatataaatg agactttaga gaatggtaaa atatacgata gtaatagata
     1621 taccttatat tcaagattat taaaacttgg aatacaagca gatacaatat gcaattgttc
     1681 tgatgatata ttggagttag aaaaaaaggt aaagaatatg ttacagagtg tagatttttt
     1741 aattaccaca ggtggtgttt ctgttggaga taaagattta attccagatg tattcaaaaa
     1801 aataggggca acagaattat tttggaagat aaacattaaa cctggtggag cttgttatgt
     1861 aggtacattg ggagaaaagc tattatttgg tctttctggt aatccacatg cagcaatagt
     1921 ggtatttgat aatgtgatag ttcctgttat tgagtattta acatataaga agggaacaca
     1981 tcatataaaa gctatattta aaggagaatt taataaattt tcaaataaag atagatttgt
     2041 aagtggaaaa ctatatacaa gtgaaggtaa attgtatgta gagctaaatg ataagaaaga
     2101 tgcaaaagct agactatctg caactctaag aagtaattgt atgattaaga ttaaaaagaa
     2161 tgagacaata aaagaaaatc aattagtgga tgttgttatt agatagtttt atgaaaggac
     2221 acaattttat gtataatata ttgtcagttg tctcatattc aggagtaggt aaaactacgt
     2281 tgattgaagg cataataaaa gaattaaata aaaaagggta tcgtgttgga gttattaaac
     2341 atacctgtca tgattttgat atggatgagg aaggaaaaga tacttataaa catagaaaat
     2401 ctggggcgag aaaagtctgt ataatatcag ataaacgagt agcatatatt gaagaactaa
     2461 aagagaagaa tccattatat aaaatgattc aattatatga agatatggac ttgattatca
     2521 tagaaggata taaaaattat agatttaaaa gactggaagt aactagaaaa ggtaagtatg
     2581 aggatatttt atctaataag tctgatttga taggtattgt tagtgatata gaatataaat
     2641 tgaatattga acagtttaat ttaaatgatt ataaaaatat atcaaagtac atagagtctt
     2701 gtataaaaaa tgaaatttta agtataagca atgttgaaat aaaaaacata ttaaaagaat
     2761 aattttaaga tgttcttctt atataaaatg atataattat ttatataaat aaagatataa
     2821 gaaatttatc aattttattt tgagttataa aatattaaag aaattgtagg aggacaatat
     2881 atgaaaaaaa tattagtagc ctgtggagca ggaatcgcaa catcaacaat agtttgtgac
     2941 agagtagaaa gattggttaa agaaaataat atacaagcag aagttgagca atgtaaaatt
     3001 gctgaatgtt caacaaaaca agaaggtgct gatttaatcg tatctactac tatactacca
     3061 acaacttatg atatacctac aataaaggca acaggatata taacaggtat aaatacaaca
     3121 gcattagata aaaagatatt aaatgcattg aagtagtttt gtatttataa aaaaaattta
     3181 gtattaaata tagagttgta tctttataaa attttaaata tagagatata gctcttttta
     3241 tgttgtttca agtatacaac tatatgatat aaatcttcaa ttttatgaaa aagattgtta
     3301 ttactttgta agtttataaa agatttaaag ttattgttat gttatactaa tttatgggta
     3361 ttttttagaa gtactaatag aagtactaat aaaggaggga tttaatactt atgataaaac
     3421 aatttgtcaa atattataag ccatataaga aaatttttac actagacctt atagcagctt
     3481 ttctattttc cttatgtgat ttagtttatc ctatgataac tagaaatata atggatgacg
     3541 ttgttccaaa taaaaactta agaatgttag tagtttttgc agtagcactt atacttatat
     3601 ttatagcaaa agcagggctt aactatttta tgcagtattg gggacatgtt attggtgtag
     3661 atatgcaagc tgatatgaga aatgaggtat ttacacactt acaaagactt ccaaatacgt
     3721 attttgataa taacaaatct ggagtaacta tgtctagaat agtaaatgat ttaatggata
     3781 taacagaact tgcacatcat gggcctgaag atttattcat ttctatagta atgttggttg
     3841 gttcattctt tatacttata gatataaata ttcctcttac gcttataata tttgctatat
     3901 taccgtttat aatttggttt gcaatagcta aaaaagataa gatgaatatt gcttttatga
     3961 agagtagagt tactataggt gatgtaaatg ctactttaga aaatagtata gcaggtatga
     4021 aggtaactaa gtctttttgt actgagaaag aagagttaaa taagtttgta agaagcaata
     4081 aattatttag aagagcaaga caagattctt ataaggtaat ggcagaatat tactctggta
     4141 tgaatttata catggatatc ttagagtggg tagttgtaat agctggtggt tattttactt
     4201 atataggaaa gattactttg ggtgattttg ctgcttatat actatatgta aaaatgttta
     4261 tacaacctat gaaaaaatta ataaacttta cagagcaata tcaaaatggt atgacaggtt
     4321 ttaaaagatt catagagata atggaacaag accatcaaaa agaagctaaa aatcctattg
     4381 aacttgaaaa tgtaaaaggt gacattgaga tagaaaatat atctttcaca tatgaagata
     4441 agactcaagt tttggataat ttaagtttat ctataaaagc tggtaagact atagcattag
     4501 ttggaccttc tggaggagga aagactacat tatgtaattt attgccaaga ttttatgaat
     4561 ttgataaggg tgatataaaa attgatggaa agagtataaa agatgttagt ttaaaatcat
     4621 tgagaaaaaa tataggtata gttcaacaag atgtattttt atttacagga actataagag
     4681 ataatattct ttgtgggaac cctaatgcca cagatgaaga aatgatagct gctgctaaaa
     4741 aagctagaat acatgacttt gtagagactt tgccagatgg atatgatact tatataggag
     4801 aaagaggcgt aaaattatca ggtgggcaaa aacaaagaat ttcaatttca agaatctttt
     4861 taaagaatcc tccaataata atattggatg aggctacatc tgctttagac aatgtaacag
     4921 aaagagaaat tcaagagtct ttagaggaat taagtaaaga tagaacaaat ttagttgttg
     4981 cacatagatt gactactata aaaaatgctg atgaaatcat agtattgacg gataaaggaa
     5041 ttgaagaaag aggtactcat gaagagctag taaacaaaaa tggagtatat agtaggttac
     5101 acaataacta gtatacaaat tttacaccat agctaacata cacattaaac ttacaaacat
     5161 aggatatatt aagattcata aagacaattc attttatatt tataagatgt tttatgaata
     5221 aaatgtatcc tatgttttat tttttgcaaa ttacatttaa ttctctttgt aagaatagag
     5281 ttaaatagcc aatggtcggg ataacgtttt tacaaaaaga ataatttaga aaactaactt
     5341 taatttatgg aatttatcga attttatgaa aaaaatcgaa ttttgacttg aaatgttttt
     5401 caaaagtagt aaaataatgg ataactatac atacatttta taaaaatata tttattatga
     5461 ggggggattt tgtatggagg caataacagc tttttgtcta gttttagtat cactgacgat
     5521 aggagatata gtttcagcaa aaactaaagc ttttgttccg tcggtttttg tatcagcaat
     5581 tatatttata gtaggatttt ggactttttt cccagaaaat attgtggact tagccgcttt
     5641 aggaacacca ttagctcaat taggtatgct tttgttaata actcatatgg gaactatgat
     5701 gagtattaag gaattagcag gtcaatggaa aacaatagta atagccttgg caggaatagt
     5761 aggaatatgt gtaggtgctt tggcattagg gacagtagta tttggatggg atacagcagt
     5821 aatagctaca cctcctctta ctggagggct agttgcatct ataatgatgg cagatgctgc
     5881 tacagcaaaa ggtcttgcaa gtttatcagt acttgcaatt ttgatgtatg ttgcacaagg
     5941 atttgcgggt tatcctatta cagcattaat gcttaaaaaa gaaggaaaaa gacttttatc
     6001 agactttaga agtggaaaag taactgtaaa tacagatgaa gaaaaagtta aggatttacc
     6061 tgaacaaaaa agcagattta agataatacc agacttacca gaaaaatatg atacgacata
     6121 tatgataata cttagattag gatttgtagc atggttagca gtacaattta caaatcttac
     6181 aaatgaagtt gtatcaagat atgttatatg tttaatattt ggtgtaatag catcagaact
     6241 tggaatagtt gatagaaaac cacttaataa atctggttca tttggatggt taatgacatc
     6301 attgatggct tttatatttg cagggttagc aaaagctaca ccagcaatgc ttgttgaaat
     6361 ggcagttcct cttgtaggaa tcataatatt tggtgtaata ggtatgggaa tattctctat
     6421 tatagtaggt aagcttttag gtgaaagtaa agaaatggct ttttcagttt cgcttacatc
     6481 actttatggt tttccaccaa actatatatt aactgaagag gcagtaaagg ctttggctga
     6541 aacaccagaa gaaaaagaat ttttaatgga taagatgctt ccaaagatgc ttgttggagg
     6601 atttactaca gttacaatag cttctgtagt aatagcaggt atctttgtaa atctaatata
     6661 gtatttaagt gataaaaagt attttaaata agaagaatgt gtactacttg gaggtatgga
     6721 atggatatta aagaaataac aaagagttat aaagactatg taattaagct tagaagggaa
     6781 tttcatgaaa atcctgagaa aagtatggaa gaagtgagaa catcaaagag agttaaagaa
     6841 gaacttgata aaatagggat accatatgta tcagctggag gtacaggtgt aattgcaact
     6901 ataaaaggag ctaatccagg aaagactgta gcacttaggg gtgatatgga tgctctacaa
     6961 gttgttgaat gcactgatgt agaatacaaa tctaaaaatg aaggacttat gcatgcttgt
     7021 ggtcatgatg gtcacacatc aatgcttcta ggggcagcaa aagtgttaaa tgatattaag
     7081 gatagtatca atggaactgt aaaattattt tttcagccag gagaagaagt tggtaaaggt
     7141 gcaagagcta tgattcaaga tggtgctatg gaaggtgtag atagtgtgtt tggaatacat
     7201 ctttggactg atgtagaatc tgggaccata tcggtagaag aaggaccaag aatggcttct
     7261 gctgatttct ttaaaattac tgtaaaaggt agaggtggtc atggttcact tcctcatcaa
     7321 ggtgttgatg cagtgcttgc aagttcagca atagttatga atctacaatc tatggttagt
     7381 agagaagtaa gtcctctgga accattagtt gtgagtgttg gagttttaaa ttctggaact
     7441 agatttaacg taattgcgag tgaagctgtc ttagaaggta caataaggct atttaatcca
     7501 gagcttagaa agcaaattcc tggaatttta gaacgtatag caaaatctac agcagaagca
     7561 tatagagcag atgctgaatt agagtatggg tacttaacac cagcagttat aaatgacaaa
     7621 gaatgttcaa agatagctac agatgcagca attaagctat ttggagaaga ctgtataaca
     7681 ctatttgaaa aagtaacagg agcagaagat ttagctgagt ttatgaatat agcacctggt
     7741 gcactagcat ttgtaggagc tagaaatgag tcaaaaggtg cttgttatcc tcatcatcat
     7801 ggatgcttta atatagatga agatgctcta gaaattggaa ctgctttata tgtgcaatat
     7861 gcagtggatt tcttaaataa ataattttaa tatactataa agaaaaagca gagactaagt
     7921 ttatatctct gcttttttat tgtgtatatt ttttataaat attgactata taaaatatat
     7981 atgatagcat aaagaaaatg ataatcaata tcaattaaaa agaaggtgat ttatttgaat
     8041 aatgtaatat ttgcattttt gataacatta ttggctggtt tatctactgg tataggaagt
     8101 tgtatagcat tttttgctaa aaaaacaaat actaagtttc tttctataag cttaggattt
     8161 tctgctggtg ttatgattta tgtttctatg atagaaattt ttcctaaagc acaagatgct
     8221 ttgacaaaat ctatgggaga gaaattagga agttggtcaa ctgtaatagc tttttttata
     8281 ggtatggcta tcattgctgt tattgataag ctaattccac aagaagaaaa tccacatgaa
     8341 ataaagaaaa tggagaatat agaagaaaag aatatcaaaa aaaataagtc tcttttgaga
     8401 acaggtatat ttacagcaat ggctatagca attcacaatt ttcctgaagg attagctaca
     8461 tttatatcag ctcttgatga tgttacaata gctattccga tagcaattgc aattgcaata
     8521 cacaatatac cagaaggtat atctgtatct gtaccagttt attatgccac aggagataaa
     8581 aagaaagctt tttattattc ctttttatct ggtatgtctg agcctttagg agcgattatt
     8641 ggatatgtac ttcttagaaa ttttttaaat gatataactc ttggaattgt atttgcaatt
     8701 gtaggaggaa ttatggtatt catatcacta gatgaattat tgccatcagc tagagagtac
     8761 ggggaacatc atttatctat ttatggattg attgcaggaa tgggtgttat ggctattagt
     8821 ttattactat ttaaatagct ttttaaatat atgattgtaa cctaaaatta gtatcaaaat
     8881 gatataatta tttaaataga tttttaagct atttaaaaat atcattttgg agaatgagtg
     8941 ctatggaaaa gaaaataaaa ttcaaatttg actggtatag ttcacctgat aagatagaga
     9001 aatggttaga agatattgaa gctaaaggat ataatttata taagataaat aaatttggag
     9061 gaatatttta ttttcataag ggaagtccaa gaaaaataaa atacactgtt gattactact
     9121 atgatgtaga gaataattat tttgatgaac ataaatcaaa tggctggaat cttgtattta
     9181 ggagtattgg tagttttatg ggtacatctg catggtatat atggtcgaaa gaatacaaag
     9241 acatacctcc taaattatat agtgattctg ataaaaaaaa caaagtaaag tcaattttat
     9301 ttatgaatat gtttatattc tttatgttag tagtaatatt tatatattta ttgaatattg
     9361 aaataagaaa tttatatagt ggttatggag catctgtaac tgctattgta ctacagatta
     9421 tattgttgat tttatatgca gtacaattat ataggagatt aatgtattat ttaaggctta
     9481 aaaagtttaa ataaataaaa gaaggtgtcc taaaattatt tttttaatag tttaagacat
     9541 cttcttttat cagtaagtac tgtttttaat tatagtaatt tctgtcttta aattga
//
LOCUS       DACULQ010000144         9573 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.145, whole genome shotgun sequence.
ACCESSION   DACULQ010000144 DACULQ010000000
VERSION     DACULQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.145"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..2469
                     /locus_tag="JA154_14340"
     CDS             <1..2469
                     /locus_tag="JA154_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAT7997025.1"
                     /translation="LEASKDKQKIKLESLNNEIELLKESIIDILNKKQEFSNKLSTLN
                     ANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNENLKNVNNRHKELSTN
                     LQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNRGVKEVLKNKNLKGV
                     HGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYLKQNNLGRVTFLPLN
                     IIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTILINNIDEGIKFAKE
                     TGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTEKISNIKNEISHLEL
                     KRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEIESLKGSITKLENEK
                     NDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKRYNDLYDKEKSEFDE
                     LNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEEHEIIKLQDSILTEE
                     KEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQHIELKESLFKVGGR
                     LERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESLKREIRSLGNINIDS
                     IKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFEIKFEEISKNFKYVY
                     KRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGGEKALTAISILFAIL
                     ITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHRRGTMEAADYIYGVT
                     MQEKAISKVISLKLKEAQEITDII"
     gene            2496..3776
                     /locus_tag="JA154_14345"
     CDS             2496..3776
                     /locus_tag="JA154_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAT7997026.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPVPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            3879..4247
                     /locus_tag="JA154_14350"
     CDS             3879..4247
                     /locus_tag="JA154_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997027.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            4244..5596
                     /locus_tag="JA154_14355"
     CDS             4244..5596
                     /locus_tag="JA154_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAT7997028.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            5634..5906
                     /locus_tag="JA154_14360"
     CDS             5634..5906
                     /locus_tag="JA154_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAT7997029.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            5971..6198
                     /locus_tag="JA154_14365"
     CDS             5971..6198
                     /locus_tag="JA154_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAT7997030.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            6306..6821
                     /locus_tag="JA154_14370"
     CDS             6306..6821
                     /locus_tag="JA154_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HAT7997031.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            6830..7525
                     /locus_tag="JA154_14375"
     CDS             6830..7525
                     /locus_tag="JA154_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAT7997032.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            7710..8060
                     /locus_tag="JA154_14380"
     CDS             7710..8060
                     /locus_tag="JA154_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAT7997033.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            8408..9277
                     /locus_tag="JA154_14385"
     CDS             8408..9277
                     /locus_tag="JA154_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAT7997034.1"
                     /translation="MDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELLDARIPFSSK
                     NPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIKGVGINKIIE
                     ECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQTGDKPGVTK
                     GKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETLALRLIEKLM
                     KIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTVLNEFRDGKI
                     GNITLEVPENVKR"
BASE COUNT         4183 a          908 c         1811 g         2671 t
ORIGIN      
        1 ttggaagcaa gtaaagataa acaaaaaatt aagttagaat ccctaaataa tgagatagaa
       61 ttattgaaag aaagtataat tgatatatta aataaaaaac aagagtttag taataagctg
      121 tccactttaa acgcaaataa agaaaatatg aatatcagag atgaaaatat aaattcagag
      181 ataacagagt taaacaaaaa tattgaaata aaatcatctg aattagatac tataaataaa
      241 gaatttaata tgcaaaatga aaatttaaag aatgtaaata atagacataa agaattatca
      301 actaatttgc aggattcaat tagtgaacac aataaactag aagatgaaat acagaaaagt
      361 aaatataatt taaatggata taattcaaag ttaaatgtct atatagatat ggaaaatcat
      421 tatgaaggat ttaatagagg agtaaaagaa gtactaaaaa ataaaaattt aaaaggagtt
      481 catggagcat taggacagat tataaatgta cctgaaaaat atgagaaatc tatagaagca
      541 gctcttggtg cttatatgca aaatataata actgataatg agtttagtgc taagtctgca
      601 ataaactatc taaaacagaa taatttaggt agagtaacat ttcttccatt aaatataatt
      661 aaatcaaata aaataagtct aggaaattta aaagcaaata ctaagttcat aggaatagca
      721 agtgatttaa ttacatttga tgaaaagtat agaaatataa ttgaaaatat acttggaaga
      781 acaatactta taaataatat agatgagggc ataaaatttg caaaagaaac tggtcatagg
      841 tttaaaatag taactttaga tggtgaaatc ttaaatccag gaggttcttt gactggggga
      901 agtctaaaaa ctaatgggaa tatactatcc agaaaaagat acattaatga atatacagaa
      961 aagataagta atatcaagaa tgaaatttca catctagaat taaaaagaga atctttagac
     1021 aaagatgtga aaaatatcaa aaatgaaatt gattcacatg aaagtaaaat aaaagattta
     1081 gaaaaaagta taataataaa aagtacaagt ataaagaatg ttgagtctga aattgaatcg
     1141 ttaaaaggta gtattactaa attagaaaat gagaagaatg atttaaattc taacctaaat
     1201 tatacacttg aaaaaagtga tgatgttaga aaagatatgg aagagttaga tgatttatac
     1261 aataaaaata aagaaaaaat tgatgcattg aatgaggaaa taaaaagata caatgattta
     1321 tatgataaag aaaaatctga atttgatgaa ttgaacttaa gtttagtgaa gaaaacagaa
     1381 gtttacaata gtatagttag agatataaaa agaatatctg gtgaaaattg tgagctagaa
     1441 gaaaaaaata aacaattaga agagtcatta aattatgaag aacatgaaat aattaaatta
     1501 caagattcta tactcactga agaaaaagaa aaagaaaatt taacaaaaca actaggggat
     1561 agtaatagaa atcttgaaac tagaaaaata gcaaaagatg atttaaaaaa tagttttgat
     1621 gaaattaata aagaattaaa aactatagat agacagcata tagaacttaa agaaagttta
     1681 tttaaagttg gtggaagatt agaaagatta aaaactagtc aagacacata tataaataag
     1741 ttgtttgagc aatatgatat gaccttagta caagcattgg aaattaaaga tgaagatttg
     1801 gatatagata gaaaattctt agaaagtcta aagagagaaa taagaagtct tggaaatata
     1861 aatatagact ctataaagga atatgaggaa ataaaagaaa gatacgattt ttatagcgaa
     1921 caaaagcaag atttagaaga gtctatggaa gagatagaaa aacttataca tactttagaa
     1981 gaaaatatga aatctgaatt tgaaattaag tttgaagaaa taagtaaaaa ttttaaatat
     2041 gtatataaga gattatttgg tggaggttgt ggagaattaa ctattttaga taaagaaaat
     2101 ttattagaaa gtgatatact aataacagct caacccccag gtaaaaaaat gaaaaattta
     2161 aacctattat ctggtggaga gaaagcttta acggcaataa gtatattatt tgctatattg
     2221 attacaaagc caacaccatt ttgtatatta gatgagattg aagcgccact tgatgacgca
     2281 aatatattta gatttggaga atttttgaag gatttatcta aagagactca atttatatca
     2341 gtaactcata ggagaggaac tatggaagct gctgattata tctacggagt tacaatgcaa
     2401 gaaaaagcca tttcaaaagt tattagttta aaattaaaag aagctcagga aataacagat
     2461 attatataac ttagattgac ataggaggaa taacagtgtt aaaaaaatta tttggttttg
     2521 gaaaagacaa ggaaaaggaa atagaaaaaa aagatgctga agaagaaata gaagttgaag
     2581 acagtgttga taatttagaa aacttggaag aaactatttt ttcaggactt gaagaagaag
     2641 tgatagataa agtggaagat gttgaagaaa aaaatgagga aagtgtcaat aatgaaggca
     2701 ctgaagaaat cgaaaacttt gaaaaaatag aagttgagat ggatagtaat ggaaaagaag
     2761 tagaaaatat ttcaaataat gattctcaag aagagataat cgaagaatta gaaaattatg
     2821 atgaaattga gtctaaagag atagaagata aagaagataa gaaagttaac ttgtttgaaa
     2881 gactaaaaca aggattgaca aaagcaaagc aaggaataac tgatagaata gatgaagttt
     2941 taaaatcata tacaaagata gatgaagaat tacttgaaga tttagaggaa attttaataa
     3001 cagcagatgt tggtgtaaat acaactatgg atataattga aagactaaga gataaaataa
     3061 agcaaaaagg tattactgaa ccaataaagg taagagaaga acttaaatct atagttgaag
     3121 atattcttac aaatgaaaac tctacactag atatagaacc agtaccatgt ataatattaa
     3181 tggtgggtgt aaatggggtc ggtaagacta ctacaatagg taaactagca aatagataca
     3241 aaaaagatgg taaaaaagtt ttattagcag cagcagatac atttagagca gcagcaacag
     3301 agcaattaga aatttgggca aatagaacca atgtagacat aataaaacat caagagggag
     3361 cagaccctgg agctgtagta tttgatgcta taaaagctgc aaaagctaga aaaacagatg
     3421 tattaatatg tgatacagca ggaagattac acaataaggc taatcttatg aatgaacttg
     3481 gaaaagtatt taaaatagta gatagagaat ttccagaagc aaaacgagaa gtacttcttg
     3541 tggtggatgc tacaacaggt caaaatgcag ttgttcaagc aaagacattt aaagaagttg
     3601 cagatataac aggtatagta cttacaaaac ttgatggaac tgcaaaaggt ggagtagtcc
     3661 ttgcggtaaa atcagaagta gatgtacctg taaaacttat tggagttgga gaaagtgtag
     3721 aagacctaca agatttcaat gcaaaatcat tttcagatgc attatttggt aactaatgtt
     3781 cttttaaaaa taatggttga caagagtgtt gttatgatat aaaatacaat agtgtaaagt
     3841 aaataacctt tacagtatac tgctaggaag tgtaagatat gaatatagaa aaaatggtcg
     3901 aaattggatt gttatttgaa cagtataaag aattattaac tgacaagcag aaagaaatag
     3961 ttgctctata ctatgaagaa gattattctt taggtgaaat tagtgaaaat ctgaatgtat
     4021 caagacaagg tgtttatgat accttaaagc gttcagaaaa aatactaaga gattatgaag
     4081 aaaagttaca cttagtatca aagattcaag agcaggaaaa aaatattaag attataaaag
     4141 ataaaattat tgacattaaa gaagatttat tgcacaatag agattgtgct aatttaatcc
     4201 ctaagctaga aaatatagaa gatgtatgta gggagatgat aaaatgatat ttgaaggatt
     4261 gtctgataaa ctacaagggg catttagtaa gttaaaatct aaaggaaaac ttacagaagt
     4321 agatgtaaaa aatgctatga gagaagttaa acttgctctt cttgaggcag atgttaactt
     4381 taaagtagtt aaagatttca taaaaaaagt tcaagagaga tgtgttgggc aagaagttat
     4441 gcaaagctta actccagctc agcatgtaat caaaattgta aatgaagagc ttacaagttt
     4501 aatgggagat gtacaaagta aagtaatgat ttcctcaaag cctcctacaa ttttaatgat
     4561 ggttggattg caaggtgcag gtaagactac tacagcagga aaacttggag gatattttaa
     4621 aaagcaaggt aaaaaacctc tattagtagc ttgtgatatt tatagaccag cagctataaa
     4681 gcaacttcaa gttgttggag aaaaattgga tatacctgta ttcaatatgg gagataaaga
     4741 aaatccagta aatattgcga aagcaggttt aagccatgcg attaaaaatg ctaatgattt
     4801 agttattata gatacagctg gtagacttca tatagatgaa gtacttatgg atgagttaaa
     4861 gtctattaaa tcagaagtta agccacatga gatacttttg gttgtagatt ctatgacagg
     4921 tcaagatgct gtaaatgttg cagaaagctt caatgaggca cttggtgttg atggagtagt
     4981 attaactaaa ttggatggag atactagagg aggtgctgca ctttcaataa gagcagttac
     5041 acaaaagcct ataaaattta tggggatggg agaaaagcta gatgatatag aacctttcca
     5101 cccagataga atggcatcaa gaatattagg tatgggagat gtcttaagtt taatagaaaa
     5161 agcacaagaa aatatagatt tagagaaagc taaagaatta gagcaaaaaa ttaaaaaaca
     5221 agagcttgat tttgaagatt tcttacaaca aatggaacaa atacaaaata tgggacctct
     5281 tgacaagata cttggtatga tacctggtat gggaaatgta aaagaccaac ttggagatgt
     5341 agatttgaat ggcaaagaaa tgaagagaac aaaagccata attcagtcaa tgactacata
     5401 tgagagaaga aacccaggag tattaaatgc ttcaagaaag aaaagaatag ctaaaggcag
     5461 tggaactagt gtacaagatg taaatagact tataaaacaa cttaatgaaa tgaaaaaaat
     5521 gatgaagatg tttgctggtt cacaaaagag tatgaagaaa aggggcggct taggcggttt
     5581 accttttttt aaataaaata taaaaaagta aaaattattc ggaggtgact gtaatggcag
     5641 ttaaaataag attaaaaaga atgggagcta acaaaaaacc tttctacaga atagtagtag
     5701 ctgattcaag agctccaaga gatggaaaat ttatagaaga aataggatac tataatccaa
     5761 tttctgaacc taaacaagta agaataaatg atgaaaaagc tataaaatgg ttagctactg
     5821 gtgctcagcc aacagaagta gttaaaaaat tacttgtaaa aaacggagta atagaaaaat
     5881 tcgaagcttc taaacaagca aaataatttt ttcttggcaa ttagtttaag taagggctca
     5941 ttgctaaggt tactttagga gatgaaatat atgaaagagc tagttgtaga tatagctaag
     6001 gctctagttg ataatcctga ctcagttgtt gttgaggaat ttgaagataa tgatggtatc
     6061 gtcttaaagc ttacagttgc tcaagaggat atgggtaagg ttattgggaa gcagggaaga
     6121 atagctaaag ctataagaac tgtcgtaaga tctgttgcaa atagagaaaa tataaaagtt
     6181 tctcttgaga tagtataaaa taggaggatt aggtataatg cctagtcctt tttatataaa
     6241 tgcgtagttt aagattttat gatgatatga ccacataaga ttaaattttt taagaaggtg
     6301 ataacatgaa agaaaaatta actcatttta aggtaggtca aatagtaaat acacaaggtt
     6361 taaaaggtga agtaagagtt tatccgctta ctgatgatat agagaggttt gatgaattaa
     6421 aagatttcta tctgggaaaa gatttaaata ataaattggc tgttgaaaaa gtaagataca
     6481 aaggttctat ggttataatg aagataaaag gcatagacag catagagaaa gcagaaaaac
     6541 taaaaaataa atttatgtat gtatctagag aagatagcag agagcttgaa gaaggcgaat
     6601 tttttatagc agatatgatt ggaatggaag ttttaactgt agatggaaaa tatgtaggaa
     6661 tactagaaga tgttcttcaa tactcagcaa atgatgtcta tgtagtaaaa ggtgaggaag
     6721 ataaagaatt tatgatacca gctataaaaa aatttgtacc tacaatagat attgatgaaa
     6781 gaaaaatgat tatagaccct attaagggaa tgatagatta ggtggtaata tgagatttca
     6841 tataatgaca ctttttccag aaatatttaa ttcgtacatg gatgaaagta taatgaagag
     6901 agcagttgaa aaaggtataa ttgaggttca tatatataat ataagggatt tttctaataa
     6961 taaacataaa aaagtagatg attatccatt tggaggagga gcggggatgg taatgactcc
     7021 tcaacctata tatgacacat ataagcatat aataacaact cataatataa ataacccaag
     7081 tgtaatttat cttactccaa aaggtaaggt atacaatcaa tctatggcaa agcagatgtc
     7141 tttaaaagaa gatataatac tactatgtgg tcattatgaa ggaatagatg agagaatcat
     7201 tgatttaata gttactgatg aaatttcaat tggagattat gtattaactg ggggagagct
     7261 cccagcactt ataatgatag attctatatc gagattaata ccaggtgtat taaaccaaga
     7321 agaatcattt gaagaagaat catttaaaga caacttattg gaatatcctc attacactag
     7381 acctagagat tttgaaggat taaaagtacc agaagtgctt ttatcaggga atcataagaa
     7441 gatagatgag tggagaagag aagaatctat aaggataacc aaagaaagac gatttgattt
     7501 atataaaaag tctaatgaaa aataaaaata taatgaaaaa ttaatatata tgtatacaat
     7561 aaagtaccta taaatggaat taaaaacata aaagtgcttg taataactat tgaagtatag
     7621 tataatataa aatgttgaaa atacgggcgt tcctctgttt acactggtcg ggtagttaag
     7681 aacgtctatg atatctagga ggaagaaaga tgaatgaaat gctaagagca attgagcaag
     7741 aacaattaaa aaatgaagtt cctaattttg gacctgggga cacagttaaa gtacacgtta
     7801 gaattataga aggaaaaaga gaaagaatac aagtattcga aggtgtagta ttaaagagac
     7861 aaggtggagg agcaagagaa actttcacag ttagaaaaat gtctttcaat gttggtgtag
     7921 aaagaacttt cccagttcac tctccaaaaa tagaaaaaat agaagttact agaaaaggta
     7981 aagtaagaag agctaaacta aactacttaa gaggtagagt aggtaaagct gctaagataa
     8041 aagaagctag aaataaataa ttaaaaactt tacttataaa tggggggctg atttatcagt
     8101 cctctttcta ttttaatgaa aattacaaca aataattata ctagaaataa atagaaatat
     8161 ttgttgaaaa tatagattat ttatctatga atttatagct tttaaaatga aaaataaaat
     8221 tggtaaatat tgcattatat tttgtaacga tattaaaaat aagagaacag tattatgtaa
     8281 agtatgacgt taaaaagatg acaatattat agtttgtgaa gatatatttt ttaagtggaa
     8341 ttaggatata ataatataat attaataagg agtgatacat gtgtcaaatg aatacgaaga
     8401 gtatcttatg gatgacaatc tacatataaa ctggtatcca ggtcatatga aaaaaactaa
     8461 ggagttagta aaaaataact taaaattgat tgatgtagta attgaacttt tggatgctag
     8521 aatcccgttt agtagtaaaa atccagacat agacaggctt gtaggagata agccaagagt
     8581 tgtcgtactt aataagagtg atatggcaga tagggataaa ttgaatcaat ggattgaata
     8641 ctataagaaa attaatatca aagctatacc agtagataca ataaaaggtg ttggtataaa
     8701 taagataatc gaagaatgta aaaatgtaac tagagaaaaa atgagttctt taaaagacaa
     8761 agggagaaaa gaaagagcta tacgaataat gatagttggc gttccaaatg ttggtaaatc
     8821 gtctcttata aacaaattaa caggtagaaa aagcactcaa actggagata agccaggagt
     8881 aacgaaaggg aaacaatggg ttagattaaa gggaaatctt gaacttttag atacgcctgg
     8941 aatactttgg cctaagtttg aagaccaaga agttgcatta aatttagcat ttagtagagc
     9001 tataaaggat gagattttag atactgaaac tttggcttta agacttatag aaaaacttat
     9061 gaaaatagaa cctgagaagt tgaaagccag atataagtta gattgtctag gagaaactcc
     9121 aattgaaact atggatatga ttggtcataa aagaggattt ataacaggta aaaaagaact
     9181 agattataca cgcatagcca caactgtatt aaatgaattt agagatggaa aaataggaaa
     9241 tataacattg gaagttccag aaaacgttaa aagatagatt ttaaaacaaa atcaaaaatt
     9301 taatgttaaa aatttaaaaa agccttttag caaaggcttt tttatttttt tgaaatatag
     9361 acaaatatct ccatataaaa gtataaatta ggttagaaag cctttaaaaa tattaattta
     9421 agacaaatat acctaatatt ttgaaaatta aaatgagtgt gattattaaa aattttagca
     9481 tattgacttg cttgtttcgt ataatatata ataagcacaa ataaatacaa tatattaaaa
     9541 atttaattta tttaatttta ttaatttaat aat
//
LOCUS       DACULQ010000145         9514 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.146, whole genome shotgun sequence.
ACCESSION   DACULQ010000145 DACULQ010000000
VERSION     DACULQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.146"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..556
                     /locus_tag="JA154_14390"
     CDS             <1..556
                     /locus_tag="JA154_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7997035.1"
                     /translation="LIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYEL
                     QWGREKLFFERKKIMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVG
                     ILNSSVYDKYFKITAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNK
                     SIDKGKVEKLQIKMDNLIMDSLGI"
     gene            788..1999
                     /locus_tag="JA154_14395"
     CDS             788..1999
                     /locus_tag="JA154_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAT7997036.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            2192..2452
                     /locus_tag="JA154_14400"
     CDS             2192..2452
                     /locus_tag="JA154_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAT7997037.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            2806..4518
                     /locus_tag="JA154_14405"
     CDS             2806..4518
                     /locus_tag="JA154_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAT7997038.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            4841..6391
                     /locus_tag="JA154_14410"
     CDS             4841..6391
                     /locus_tag="JA154_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT7997039.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            6417..9119
                     /locus_tag="JA154_14415"
     CDS             6417..9119
                     /locus_tag="JA154_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HAT7997040.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSAQQIVHKQCANDILSCIENCGLDKKYISVEVTEKVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQITKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
BASE COUNT         3819 a          859 c         1671 g         3165 t
ORIGIN      
        1 gctaatatac tcaaatgata tagataatga gaatacaaat aaaaggatac tagatgaaat
       61 tatagggcta tataaaacaa aattagaaaa cagaagagaa tgtaaaagtg gaataaggaa
      121 atggtatgaa cttcaatggg gtagagaaaa gcttttcttt gaaagaaaaa agataatgta
      181 tccttataaa tcaaatgaaa atagatttgc tatagattat gataataatt tttcaagtgc
      241 agacgtatat tcttttttta taaaagaaga gtatttagat aaattttcat atgaatatct
      301 agttggaatt ttaaattcca gtgtttatga taaatacttt aaaataactg ctaaaaaaat
      361 gagtaaaaat atatatgatt actatcctaa taaggttatg aagataagga tttttaggga
      421 taataattat gaagagatag agaatttatc aaagcaaata atatcaattt tattaaataa
      481 gtctatagat aaaggaaaag tagaaaaatt gcagataaaa atggataact tgataatgga
      541 ttctttaggt atataagcag ttgtaagtat taaaaaatta taatctttta attgtttatt
      601 taaatttaga aataaaaagt tagccatata aaaaatatta aaaatatata cttttcaata
      661 tttttttgat aaaataagaa tgaatttgtg caattttatt tttctccaat aactaaaaat
      721 agttttttga attaatattt gcttaagaat aaaaatcaga aacgtttttg atatagtagg
      781 tgataatatg aaaggattag ttttagaagg tggaggaaca aaaggagcct accaaatagg
      841 tgcatataaa gctttaagag agcttggtat agagtttcaa ggagtaactg gcacatcaat
      901 tggtgcttta aatggagcat atataattca aaatgatatt gaagtaatgg aagatatttg
      961 gttaaattat gactatactc attttatgaa tattgataga gaaacctatg agaggtttaa
     1021 aaatgtagat ttcactacta aaaatctcaa tgcagtgttt gagcttataa ataaagctag
     1081 aaaaaatgaa ggtatagata taagcccatt aagacaatta atggagacaa cattaaatga
     1141 agacataata aggaaatcaa aaaaagactt tggacttgtt acggtctggt gggataaaaa
     1201 aattaatcca tatccattgt atatcgaaga tatacctaaa ggaagattga ttgattattt
     1261 aattgctagt tccagtttgc ctatttttca aacgaacatg atggatgata agttgtatct
     1321 tgatggaatg ttttgtgata acatgccaat aggactcttg gaagacaaag gttatgaaga
     1381 tgttgttgta attaggttga ttgatgattt tattgggaaa atgaatctaa atcgacatca
     1441 aaatataaat ataaaaacta taataccatc agaatatcta ggaggttctc ttaataaaga
     1501 tagagataat gtagaaaaaa acatccgact aggttactta gatactatga aagcatatga
     1561 aagatgcact ggaattaagt attattttga tgtagagtat aaatataatg aagaatattg
     1621 ttttgataaa tttagaagta tgagcaatga aacaatacaa tatatctgta atttattgaa
     1681 tataaaaaga gatgcttcac ttagaactgt tatggaaagt gtaataccaa agcttggtga
     1741 ggtgcttgga ttgccaaagg atttttcata taaagatttg ttttactgta tatatgaaaa
     1801 gaaacttgaa gaaaataata taaacaggat aaaattatat gattttaaca aggttataga
     1861 tgttgtaaat acaaatattg attctaatag aaatttagct tctaattttg aagttagacc
     1921 taaaattagt attaaaaaga acaaaaacac aaaattattg acaaattgta ttataaaaga
     1981 cataaaaaat caaagttaaa taaaaaacaa aaaaaaattt gtaattatac tcgaaaaaag
     2041 tgtatattag tagtataata tttttgtagc actagtggga acaaaaaatg accataggac
     2101 aaaaaagtac cacttagtac cattaaagta aaaaacagga aaacaatttt aaaaaatata
     2161 aaatgtaaag tttataggag gatttaaaaa aatggaaaaa gtagttagta tcaaaaatgc
     2221 aagtggatta catgctagac cagcaggaat gtttgttaag aaggcagctg aatttaaatc
     2281 aacagtagag gttgtagcaa aagacaaaac tgtaaatgca aaatcaataa tgggaataat
     2341 gagtttaggt ttagctcaag gagaagaatt aaagttagtt gtaaatggag aagatgaaga
     2401 agtagcttta gctgcaatgg ttgaattagt tgaaagtgga tttggagaat agatataata
     2461 attttttaaa aatttgatat ttagaatcta atattactta aagtaaagta acagagaagt
     2521 attaaacata tacagaccaa catttgtaga tatttaattt ctttgttact ttcatgttta
     2581 tttaatcaga agattaagac tattgttaat tgggtataaa aatataaata ttttagaaaa
     2641 tgaaataaga tatttttaat cataaattca gagtaaaaat acagtaacaa atagtttata
     2701 aaattttata aaaaattata agtaaataaa actataaaaa tgtaaattat ttatataaat
     2761 ttataaagtt tatgtgctag atgatatttt ttaggaggta gagttatggc ttacaaagga
     2821 ataggtgctt ctcctggtgt tgctttaggg aaagcgttag tagttgaaca tagtgaatta
     2881 gtaattgaaa aaaaatctat agataatgtt gaagctgaaa ttgctaaatt agaagatgca
     2941 gttgcagttt ctaaagaaga attagtaaaa gttaaggaaa aggcttctga agaattagga
     3001 gcagaggaag cagaaatatt tgaagcacat ttattagttt tagaagaccc agagttaatt
     3061 ggttctgcaa tagataaaat taaaactgaa agtgtaaatg cagagtatgc tttaaatgaa
     3121 ataaaagaga tgtttgttag catgtttgag tctatggaca atgagtacat gaaagaaaga
     3181 gcggcagata taaaagatgt tacaaataga atacttagac atatattagg aattaaagtt
     3241 gttgatttat ctgctttaag cgaagaagtt gtattaatag cacatgattt aactccatca
     3301 gacacagcaa ctatgaataa aaaaatggtt ttaggatttt taactgatat aggtggaaga
     3361 acttctcata ctgctatcat gtctagaact ttagaaatag ctgcaatcgt tggattaaat
     3421 gatataactt caaaagttaa ggatggagat tttgtggttt tcaatggaga cacaggtgaa
     3481 gtaatcgtga atcctgatga agagactata aataaatata ctgaattaaa agctaagtat
     3541 gaagatgaga gaaaagcatt acagctatta aaaggaaaac catctgtaac tttagacgga
     3601 aaacatgtgg aattagctgg taatatagga acacctaacg atatagaagg tctaataaag
     3661 aatgatgctg aaggtgttgg tctttataga acagaatttt tatacatgga tagagattct
     3721 ttcccaacag aagaaataca atatgaagca tacaaagctg tattagaagg aatggatgga
     3781 aagcctatag ttataagaac attggatata ggtggagata aagaattaag ttatttaagt
     3841 atggaacctg aaatgaatcc attcttaggt tacagagcaa taagactttg cctagataga
     3901 aaagatatat ttaagactca attaagagct ttatatagag ctagtgttca tggaagatta
     3961 agaatcatgt tcccaatgat ttcatcttta gaagagttgt tacaagctaa agaagttgta
     4021 aaagaagttt tagcagaact tgattctgaa ggtgtggctt atgctaaaga tgtagaaatt
     4081 ggtatgatga tagaagttcc atctgctgct gtaatatctg atgtattggc taaacacgta
     4141 gatttcttct ctataggaac taatgattta attcaataca cttgtgcagt tgatagaatg
     4201 aatcaaaaga taagctatct ttacaatcaa tttaatccag ctgttttaag acttataaag
     4261 actgttatag acaatgcaca taaagaaggc aaatgggcag gtatgtgtgg agaatctgct
     4321 ggagaccaaa aaatgatacc aatactttta ggtatgggat tagacgagtt ctctatgtct
     4381 ccaatatcaa tacttcctgc tagaaagtta ataacttcag ttaaagagtc agatatgaaa
     4441 aaattagctg atgatgtact taatatgggt actgctgaag aaataaaaag ctatatagaa
     4501 aagactttta atatataatt aatgttatat agttactaaa attcattttt ctaaataaca
     4561 aaattatatt ttacgaataa aaaaactata gtatatcata tatactatag tttttttgtt
     4621 atataaatta cgtatcaaaa tttgtaaaat gaatagatat aattactaaa tatgtgatag
     4681 tattggctat aataggatat aaaaaataat attgtaaaaa aaagtttata tttagtaatg
     4741 aaaatttaac gttttacttg aaattatgtg ttttgtaatg tattattaag gctctaatag
     4801 gaacattttc atcaattttt ctatagtggg ggtaggaatt atgaggtaca aaaaaagaaa
     4861 tatagataag tcaagtgaac tttcatttca atctctagca aaatatttta taactttcta
     4921 tatagtaatt atattaatta ttctttctat gttttgtata aatacaatat gggttatgaa
     4981 aaaagataat caagatttat tatctacaac atcaaatcgt attgaagata gggtaggaga
     5041 aacaattaag ctattgaact ctttgagtaa agataattac ataatagata atagtgtgaa
     5101 tggcatagat aaaagtaaag aactttcaat gtatagtgat gaatttgggt atatgatgat
     5161 atgttttgtt gataagaata taattgtgtg ggatgaagaa ggtggtaata ttagtctagc
     5221 tggtcgtgat tatatgcaga aactttttgc aacaaaaaaa tatcaagtta cagatagttt
     5281 tgcagcaggg gctgatggaa aaactttaaa ctacactatt gcatcaccta taataaagaa
     5341 tggaaatgta gatggttgtg tatttgcagc aatttacttt gatgagataa aggatatact
     5401 caaacaagga aaaggtcaac agcaacagaa tttcatatta tttggaagtg aaaatcaaat
     5461 aatgaattta acaagtgatg aaactgcata tggagagaag tatatgaagt tatcgcagaa
     5521 aacaaaatat cttggaacca atgcaaaaaa agtagaaaag gatatgttta ataataaatc
     5581 aggtgattat tgggcttttg aaaatggaaa attatacttt atgtcctata aaccaattaa
     5641 gaacacaaat tggagtttat tttgtaaagt taatttctta gatatttttg cattaaattt
     5701 aattcctatg ggagctattc tatctgttat aactttagta tttattattt tgatttgtat
     5761 ggagaaaagc ttcatgaaaa aacgaatgga agttattgat atgcttttag gttctgttca
     5821 agaattagaa aaacgtatat atcaaacaga aagacctagt gaagtagact ttaaagaaat
     5881 tattcagctc acaagtaaag gtctgactga tggattaact ggtgttgtaa cacgtactgt
     5941 attttttgac caaatttata atcgtgttga aatggaggaa aatgataaat tatctgtaat
     6001 ttcatttgtt gatttagatg atttgaaatg tattaacgac ttatatggac atgaaattgg
     6061 ggatatggtt ttgaaaaatg ttggatatgt acttcgtgaa tatgagataa aatataatgg
     6121 tttaattggg cgctatggtg gagatgaatt tgttatgttg ttatctggtt ttacatcgag
     6181 agaagaggtg gaggatttat tggatgagat tgctattcgc ttacatactg aagtactaac
     6241 agatgatggt atcattcaaa ctcattgtag tgttggagct gtagtatgta gtgtaaaaga
     6301 caataaagat atagataatt tattaaaaca ggctgatgaa gctttatact atgttaaacg
     6361 taatggaaaa ggtaattatc atttagttta atgtatgtgg aaggagttta tatcatatga
     6421 aaaatgtatc taaaaaattt tttgtatgta ctattattgt attaataaca tttttatgtt
     6481 ttagtaaatt tgctatttat gtttttgaca aacaagaaaa gaaagaaaca aaagtagtag
     6541 aaaacatgtt aaatgtctac aagaataaca ttattttaaa tctgactgaa aaatttaatt
     6601 atagcaaaga cttgacatca gttttagttt caaacaatta ttctatagat aaattaaaac
     6661 ctattgcaga tgaaattctt aaaaacaatt cttgcatata tatttcagtt gtagaaaaat
     6721 ataatgtagc tgcatttttt tctaattctg tcaaaaaaga tattatcaat actccaatac
     6781 aggaactgaa ttattcttat tcattagcaa attctatcaa aggattagtc gtagaaggtc
     6841 cttctaaaga ccagttttca aatggagatg tgtttttaat tactaatcct atttataaaa
     6901 atggagagta ctggggagaa attacagtag ctttagatgc caaaaaagtt gtagattctt
     6961 ttaacttaaa taaattaaaa gaatctggat ttgagtatga actttggcgt gtacacctag
     7021 ataatggttc aaaatctaca atagaaattt ctactaaaga ttttaatttt tccaaatcac
     7081 atgaaattaa gtttaaactt ccgactgttt ggactcttag cattgtgtac aaaaatggat
     7141 ggaataataa gaatatagat tttattgtat ttgttatatt gttaattatt tctatattaa
     7201 taggtgtgat gacatttatt tacattgaaa agaagtcgat tagtaaggaa ttaaatcaac
     7261 agagtcttaa aaatttagaa actggtattt tgaatagaga aggtttttta attaagttgc
     7321 aagagcatct gaatagaaaa aataatgaaa tagtgttgct gtatatatgt attactaatt
     7381 atagtaaatt tgctccagtt atgagctttg aaaaaagaca agattatctt acacatatag
     7441 taaaaaatct agataattac ataaaaagtt ctcattttat tgctcgtata agtgaggaag
     7501 gttttgttct agctttagaa gaaaattgtg ataaaaatga attgcttaac attgaaaaag
     7561 ggatttcctt agagctttta cgaaaaatta aagtagaaaa taaacaagta tttttaacga
     7621 ttggttatgg ctcatctagg tgtgttgatg atagaaaaga tgcagaatct ctgttacttt
     7681 cagctattga agattatgag agaagatttc cagcaatgtc tcctattcat gattttacta
     7741 aaaagtgtga agatatgctt gatgaaaaga aacagattgt atttggtgaa tatccagatt
     7801 tagaaataaa caagttgtct aaagtaatat acaaatatta taaaaaagta cagcgtatta
     7861 tgtataaaga ttataatatt gatattggaa ataggcgagg gtatatgcgt gatgtcgaag
     7921 tattaattga ttataattct gagagaatgt tatacttatt tttaatagac atatgcaagt
     7981 ttggaaaatt taatgaactg ttttctattg aaataggaga tgttatttta aaagaagtta
     8041 gtaacaaatt aaaaacaatt ttttctaatt atctatatcg tattaatgga gatgtatttt
     8101 taggcatttc aatagataaa gaatcttact tagatacttt acataaggtt aatgaagtat
     8161 ttaatagttc tttaagtgta gaagatgtac tttttgatgt aaaatgtaat attggtattt
     8221 gtcaatatcc atatcatggt gaaaatccat atgaccttct ggaaaaggta caaattgcat
     8281 tggagtatgc aaaaaaggat gtcaaaagta atgttaaagt ttatgataag gaattgacag
     8341 aagagattta ccatgaaaat aatattatta aggatttaaa aaaaggtctg gaagaacaaa
     8401 cactagaagt ttggtatcag ccaattttta atgttagtaa aaactgttat ataaaagctg
     8461 aggcactaat tagattgaga aataaagagg gcggttatta ttctgcctat gaagttatta
     8521 cttatgcaga aaagcatgga cttattaagg atgtaggtct atatgtacta aaaaaggctt
     8581 gtgatgtaac tacaaaaata aaagataaaa atatagactt tgaacaaatg caattaaatt
     8641 tatctgctca gcagattgta cataagcagt gtgcaaatga catattatca tgtattgaga
     8701 attgtggtct agataaaaag tatatttctg ttgaagttac agaaaaagta cttatggaaa
     8761 cttttgaaat tgtaaatgtt actcttgata aactaaaaaa agaaggcatt agcatagctt
     8821 tagatgagtt tggttctgga tttagtagta taaattatct ggcaaattta cctattgaaa
     8881 atttaaaaat agataaggaa ttagtagaac aaattaccta ttctaaaaaa caatttgaat
     8941 tattaaaaac tatagttcaa attactaaga ttaatggcat ggatattgta gcagaaggtg
     9001 ttgaaacaaa agaaactctg gatttagttg taacttcagg tgtgaatttg attcaaggtc
     9061 attatttttc agaaccttta agtgaagata aatttattga acttatgatt gaagaataaa
     9121 ggttaataaa ataaagattt aaggtattga aaaataatta aaagtattta ataatagaat
     9181 tagggaagaa taagtttata agtaaaaaca attgattagt agttaataaa ataaatgtga
     9241 tatccaaata aatcctaaat ctaatttgag ttttaatata gatagtctaa agtttgatta
     9301 ttaaagattt aattgtaagt tgttacagtt gatgatattg attagagatt aaagtttaga
     9361 gtagaaagtt accttaaatt taaaaatgca agataaccca atctaaaaat tataatcact
     9421 cgataggttt aaggatttat ttgtttatta taaataggag ggtgtctcaa aatgaacttt
     9481 ttagtttatg aggcactctc ttttatatag aatt
//
LOCUS       DACULQ010000146         9425 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.147, whole genome shotgun sequence.
ACCESSION   DACULQ010000146 DACULQ010000000
VERSION     DACULQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.147"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(85..738)
                     /locus_tag="JA154_14420"
     CDS             complement(85..738)
                     /locus_tag="JA154_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7997041.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPIAEMKKEKLL"
     gene            complement(983..2848)
                     /locus_tag="JA154_14425"
     CDS             complement(983..2848)
                     /locus_tag="JA154_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HAT7997042.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2853..5294)
                     /locus_tag="JA154_14430"
     CDS             complement(2853..5294)
                     /locus_tag="JA154_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAT7997043.1"
                     /translation="MIKGVVFLVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVL
                     CSLLKDEIAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGI
                     NLDDLIETEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQ
                     VEVPDNWLTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDI
                     PIIGYQNQCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYP
                     DDSNYAGKLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIP
                     ELMRILLDEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEE
                     INRRYVEELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEV
                     LKDFYQDEPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFK
                     NDSSVLQRLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNV
                     LHMYHELLDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLK
                     VVFLENYGVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEI
                     CEQVGKENMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEE
                     GRSIYNSLTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSD
                     RTISEYAKDIWFKRV"
     gene            complement(5287..6729)
                     /locus_tag="JA154_14435"
     CDS             complement(5287..6729)
                     /locus_tag="JA154_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch synthase"
                     /protein_id="HAT7997044.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6758..7888)
                     /locus_tag="JA154_14440"
     CDS             complement(6758..7888)
                     /locus_tag="JA154_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HAT7997045.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7890..9050)
                     /gene="glgC"
                     /locus_tag="JA154_14445"
     CDS             complement(7890..9050)
                     /gene="glgC"
                     /locus_tag="JA154_14445"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087356.1"
                     /note="catalyzes the formation of ADP-glucose and
                     diphosphate from ATP and alpha-D-glucose 1-phosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAT7997046.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         2989 a         1716 c          915 g         3805 t
ORIGIN      
        1 gtatttaaag ttaaatttat tcttgttttt cctttaatat ttaatagtaa aacaacaaaa
       61 ttataaaaaa aataaagaga aactttataa aagcttctct tttttcatct ctgctatagg
      121 tatcgttttc caatgttctc tcaaaattct ttcaacattt tctaaatcat tctccctcat
      181 agattttact acttcaatat gttgattatt gctgtatgtc aacattttat ataactcatc
      241 ttcattatca ctagtatatg actttgacat aaaattatat cttaaagagt tcaatagatt
      301 tactaacacc tgattatctg atttagttat gtatatatcg tgaaattcat tacttaattt
      361 taaatagcta ttgtaatttt tatactttat agagaccgcc atcatttctg ttaattcttc
      421 catttttact aggtctgatt cagttaaatt ttgtaatgct gaagaaccgg ctaacgcatc
      481 caatactcca attacggaat aggtgtctaa tttctccttt tcctgaaatt cttttactac
      541 aaatccctta ttaggtattt tttctagtaa attttcacct gctaacctag ttaatgcttc
      601 tctcgctggt gttctactaa ctccgataga tttacaaatc tcagattcag taattctctg
      661 attagcttca tattctccat tttggatttt ctttgatata tagtcatata cttgagttgt
      721 aagtggatct accttcataa gacgttcctc catattaaga ttataaaagt taaatctccc
      781 tacaactttg gtagagagtt cagttttatc ttactaattt atataatatc acaattatga
      841 tattttttat acttctttgt aatattttga aatactaaaa ttcttatgat aatttataca
      901 aaaaaagtag aaaaagtttt gataaaacaa aactctttcc acattcgcct tattttctat
      961 ttatttttct aaaaaaagat atttaattta ctctaaaaat tctaaatgat ttagagtcta
     1021 aatctacttt caactcatta ttttcaattt gtatagtact atttgcattt ttaagactat
     1081 actttatagg tatttcctca ataaaactac tctctataca taatgatata ctttccacat
     1141 tttcacttct atttactaaa attaacattc tatcattatt ttcattaact ctaataaaag
     1201 aaaatacaga atcattatta gtatatataa attctgtatt tccagaactt aaaagtttat
     1261 tcttatttct aatttttata acatctctat aaaaattaag catatcttcg tcttcatttt
     1321 tccatggata agttcttcta ttatctggat caacacctcc acaaagacct gcttcatctc
     1381 cataatatat atatggtact ccttcaaatg tcatctgtat agctacagca agcttcacta
     1441 aatctacatc atcatttaat tccgttttag ctcttgtaac atcatgtgtt ccaattaaat
     1501 ttaaattaga cctaaatgat tcttttggat aattttcttt aatctgcata tatctattac
     1561 aaagttcata tgaattaatt tctcctttta agaaagaaaa catgttattt ctaaaaggat
     1621 aacccataac agaatctaat tcttcaccta ataaataact tcttctctga ccatagctaa
     1681 tcttatttga tgcatcttcc catacttcac ctataagaat tgagtcatca tctgcctttt
     1741 ttaattcctt ttttaactct cttataaact tagttggtaa ttcgtctgca acatctagtc
     1801 tccagccctt aatacccata tttacccatt tatttataac gctatcattg tcatatatta
     1861 tataatccat atagctatgt tctaattcat ttatatttgg caatgtctta acatcccacc
     1921 aacttttata tttttgagga aattcttcaa acatatacca tgaataatat ggagattctt
     1981 tagattgata tgctcctaaa ctattatagt ttccatacat attaaaatat ttactatctg
     2041 ctccagtatg actaaaaact ccatctaata ctattgatat acctttttct tttgctttat
     2101 caataagttc tttaaatgta tcttcatctc caaacattgg gtcaattttt ttatagtcac
     2161 ctgtatcata cttatgatta ctagaggctt caaatatagg gcttagatat agtatactta
     2221 cacctaattt ttttaaatat ccaagtttat tgattatccc tcttaaattt ccaccatgga
     2281 aatcccatct tataacatcc ccttgactat cctttatgta cataggtgta tcttcccaat
     2341 ttccatatat gaaactattt tttttaggat tatcaacctt accactacga tttccattat
     2401 tgaatctatc tacaaatata tgataaagta ttccttcttt gtaccatgtt ggtactttaa
     2461 aatctttata aacagtgatt tgatatttat taatatcact ataattatat tcacaagaat
     2521 atccattatc tctgtttttt ccataaaata aagtctttat ttgaccatcc atttcaacat
     2581 caactttaaa gtaataaaaa taaacacctg taccatcaaa cttttcaatc ttaccacaaa
     2641 agcactttcc aagtttatca ttatctaatt cttcatttaa agttattgtt ctcatttctt
     2701 cttctctatt tatttctagg gaaatagact tgatattgta tccctcattt actttaacac
     2761 aaatggtcaa ttcctcatca aattttaaag ctccaaacgg agccttaaaa tttttatccc
     2821 aagagttata ttctattaca ctttgcattt aatcacaccc ttttaaacca aatgtctttt
     2881 gcatactcag aaattgttct atcagaagaa aaaaatccag agttagctat attaactaaa
     2941 gacattttat tccatttttc tttatctcta tataacctgt ttatatctgc ttgagcttga
     3001 ccataatttt caaaatctct caatacaaaa tactcatcat tgtaagtagt gagtgaatta
     3061 taaatacttc ttccttcttc gccaagatta ggaataaatc catttattaa atcatctact
     3121 acccttttta tatccatatt agaatgatat aggtcaagtg aagaatatcc accatattta
     3181 ttataattta gtacttgttc tgcactaagt ccaaataaaa acatattttc tttaccaacc
     3241 tgttcacata tctctacatt tgcaccgtca agtgtagcca aagttatagc accattcatc
     3301 ataaacttca tattacttgt tccagatgct tccttagttg tagttgaaat ttgctcacta
     3361 acatttgctg ctggtattat tatttctgct aatgatactc cataattctc taaaaatact
     3421 acttttaact tgtcctttac tctaacatca ttatttatag tacttgcaac agaatttata
     3481 aatttaatta tacatttagc caaataatat cctggtgctg cttttgcgcc aaatataaaa
     3541 gttcttgggt caagattcaa attaggatta tcaagtaatt catgatacat atgaagtaca
     3601 ttaaatatat tcagtaactg tcttttatac gcatgcaatc tcttaacttg aacatcaaat
     3661 atcgaacttg ggtctacatt taaatcgtat ttatctttta taaactttgc tagatttgct
     3721 ttattattgt attttatatc atcaagtctt tgtaaaacag aggaatcatt tttaaatttt
     3781 tcaatatttt ttaattgtaa tgtatctttc ttccatgaat caccaattaa atctgtaatt
     3841 aaattgctaa gttgaggatt tgaacttata agccatcgcc tatgtgcaat tccattagtc
     3901 ttattattaa atttatttgg ttcatcttga tagaaatctt ttaatacctc tgtttcaaga
     3961 atatgagtat gaagcttagc aactccattt acactatgac ttgtgacaat acataagttt
     4021 gccatattga tattgtcaca gtctattata ctcattcttt ttattctatc ttggtcatat
     4081 cctttattat ttaattcctc tacatatctt ctatttattt cttctattat catataaatt
     4141 cttggtaata gctctttcat catacttaca ggccattttt ccattgcttc tgacattata
     4201 gtatgattag tataggacac tgttttttta gttatttccc atgcttcatc ccaagataaa
     4261 ttttcttcat caagcaaaat tctcataagt tctggtatgc ataaggtagg atgtgtatca
     4321 tttatatgaa ttgctacctt atcgaataaa ttattaatat ttaatttgtt ctttttatat
     4381 tttcttatta tatcttgtat ccctgcactt acaaaaaagt attcttgttt caacctcaac
     4441 aacttacctg catagtttga atcatcagga tataatacct gtgatatttc ctcagtataa
     4501 tacttatgtt ttaaagcctc ttcatagcta ccagaataat ttagagcatt tgaggtaagt
     4561 tcaccaaaat ctctctttgg tatttcactc ttaaacaatc ttaaagtatt tatacattgg
     4621 ttttggtatc cgattattgg tatatcataa ggcattgcca ttacaggaag gtagttttta
     4681 tgaattactt ttaaatgact accttccttg attaattcta cctcaccacc aaacttcacc
     4741 attgttgctt catttggtct tacagtttcc catgcatatc gtccttctgt aagccagtta
     4801 tcaggcactt ctacctgata accatttaca aatttttgtt caaacagacc atatttatac
     4861 cttattccat atccttgacc acttatgttc aaagatgcca tagaatctaa aaagcaagct
     4921 gctaatctac caagtccacc atttcctaag gctgggtcta cttcagtttc aattaaatca
     4981 tccaagttga ttcctaattc agataaacct tctctaactt cttcttcaat atttaaattt
     5041 aataaattag attttaactg tctccctatt aaaaattcta aactaaaata ataaacttct
     5101 tttttcttat ctaactttgt agcaacccat ttttttgcta tctcatcttt caatagacta
     5161 cataatacat ttaacaattg ttcatcagta gcttccttga tagattgtgc atataagcta
     5221 tacattttta cttcaaattt ttgcttgaat ttctttttgc ttattgttac caaaaaaacc
     5281 actcctttaa tcacatatgt ctctatatgt ttctatatac tccttagctg attttttcca
     5341 actattatct gttttcattg cattttcaac taatttaatc catttttctt tgtccttaaa
     5401 tactttgatt gcatttttca aacagaaaaa catctcatga gcattgtaat ttttaaaact
     5461 aaatccattg ccttctccag taaatttatt ataaggtgtt actgtatctc taagccctcc
     5521 agtctctcta actattggta aagaaccata tctcatagct aacatttgac ctataccaca
     5581 aggctcaaat aatgatggca ttaagaacat atcacttgca gcatatatct gttgagccaa
     5641 tgatgcatta aaagtaattc tagctgatac tttatctgaa aaattagaat cgtaatagtt
     5701 gaacattgat tgatattgat gttctccagt tcctaaaacg acaatctgta aatcttctct
     5761 cataagttct ggcatcatgt atgctattaa atcaaatcct ttttgagaaa ctaatctcga
     5821 aactatacct atcaacggaa tagatgaatc ttttttaagt cccaatatat cttgtaatct
     5881 caatttattt tctacttttt tgtcaattga gtctacatca taatgaacaa atatatcttt
     5941 atcttttgca gggtcattta aatcataatc tataccattt aatatacctt ttaattttcc
     6001 tgactctttt ctaagtaatc catctaattg ttcaccataa aaagatgttt gtatttcatt
     6061 tgcataagta ggactaacag ttataatttt atctgcaaaa tttataccag ctttcataaa
     6121 atttattgag ccataatact ctatatctcc attatcaaag tatttttgtg gtaagtccaa
     6181 tatatcatat aaaacatgct tatcaaatac accttgatat tggagattat gtatagtata
     6241 catagttttt atatttttat acccctcaag ggttgcatat ctttctttta gaattaatgg
     6301 taacatccct gtatgccaat catttatatg tattacatct ggataaaaat caattctact
     6361 tatagcttct aaaactgcat ctgtaaagaa agtatatctt tctgcatcat ctccatatcc
     6421 atagatactc tttctttcat cttctcttct aaaataaaat tcattatcta taaagtaaaa
     6481 ttttacacca tctaattcca tttctaaaag accacagtat tgatttctcc atccaacacg
     6541 tacactaaac acagctattt cttttagttg atcttttaaa taacttggta tagttgaata
     6601 tttgggcatt attaccctta catctacacc ttcttttttt aattctttag gcaatgcata
     6661 agaaacatct cctaatcctc cagtttttgc aaagggccaa cactctgctg ttacataaaa
     6721 aaccttcatt agtcattccc ccttctaaaa aatttattta tatatttgta ttcttttcca
     6781 ctacaagagg atatttttca tctcctctta attctttccc atttgataca ctcgtatttt
     6841 tatctaatat tgcattatat agttttgcat ttgactttat tacacaattt tgcattacta
     6901 ttgagttctc tataactgtt cctttttcaa catttacctt tctaaatatg atactatctt
     6961 ttacagtacc ttctattaaa caaccagatg ctataaagct attttctact ttagcactat
     7021 ctgtataaat tgtaggtgat tcattctttt cttttgtaaa tatctttctt tcagaatata
     7081 ataattcatc tgcaacttct aaatttaata aatctttgct catttcaaaa tatgattcaa
     7141 cagaatttac acattttaga tatcctgtat attcataggc tcctattttc atattttctg
     7201 cttcttcaat aataaattct tctaaataac aataattgcc tttatttaca atgttatata
     7261 tacattcaac aaaatcttca cgcttcataa tatatgtctc catacatatg tttgctctag
     7321 ggaacttacc taaattattt cctacattta ttaccctatt gttactatct aaatttagag
     7381 ttaagttacc agcaaaatct ttatttgcat tatttacatt tttatatatt atagttatat
     7441 cattatgaga ttttttatgg tattccaatg cttgagaata atttaaattg catagcatat
     7501 agcttggagc tattaaaata tattcttctt cactcttttc tatataatct agattagcta
     7561 agatagtata tatatcacct tctctatgtg agtaagtatt tctatactta gtgttttctg
     7621 gactaaaaat tgataggcca cctttttttc tacttaaatc ccaatcttta ccactaccta
     7681 tatggtctgt taatgatctg tatttttctt ttgcgaatat tccaacttta gtgattcctg
     7741 aatttatcat attagataaa gcaaagtcta ttattctata tcttccagct attggtgtag
     7801 atgctattgg tcttccatag ttaagtttgt ttattgctgg gtctcctttt ttgtttaaat
     7861 ttattatccc taaacattca tttctcatct taaagacctc cttctattaa tacgcatcta
     7921 ggttcgatat tttcatactc agaaactaca tttatttcat tatttacatt ctttatctga
     7981 acattatcat ttatagttgc acactcacct atcatagctt tttctatagt tacattttca
     8041 cctattacaa catttggcat tattactgaa tcaattactt tacttccttt accaacaaca
     8101 actccatgtg ataatacaga gttacctact tcacctaaaa ttctacaacc atcagctatc
     8161 attgatctat gaacatttgc atttttacct atatattgtg gaggcattgc catagtattt
     8221 gtatatatct tccacttagg gtctgctaaa tcaagtgttt ctggagattt tattatatcc
     8281 atattagcat cccatagact ttgtatagtt cctacatctc tccagtatcc tttaaatgga
     8341 taagcataca ttccaacatt atcttctaac attttaggta ttaaattttt tccaaaatca
     8401 tcataattta tttctggatt tttttctgct tccttaaaat aatttctcaa cattttccaa
     8461 tcaaatatgt atactcccat agatgccaaa ttgctcttag gttcacttgg tttttcttca
     8521 aattcataga tacttgaatc ttcatttgta ttcattatcc caaatcttga agcttcatcc
     8581 caaggaactt caatcactgc tattgtagct ttagagcctt tttccttatg aaattttaac
     8641 attttgctat aatccatctt gtatatatgg tcaccagata atatcaatac atattctgga
     8701 ttataggtat ctacaaagtc catattttga tatatcgcat gtgctgtacc attataccaa
     8761 tttccttctt tttcattcat aaatggttgt aaaacataga caccaccatt tattctgtcc
     8821 aaatcccaat gacttcccat gcctatatgt gaatttaata tcaacggtct atattgagtt
     8881 aaaacaccta cagtatcaat accagagttt gaacaattgc ttaacacaaa atctataatt
     8941 ctatacttac ctccaaatga tacagctggt ttggcaattc tttttgtaaa aacacctaat
     9001 cttgaacctt gccctcctgc caaaatcata gctaacatct cttttttcat ttaatgacac
     9061 tcccctcatc tagtaattaa tatttataat tgcattttta cttttataaa ctttatcagt
     9121 caataaatgt cgtgtataaa tgttgcttaa ataatacctt aataaaaata aaataatatc
     9181 aactatacaa ttgatataat gtatacccca taatggactt accgacgtat acattatttt
     9241 ccataagcac tatagtattt attaatattc aattattggt taaaaaataa tacaaatttt
     9301 attttataaa tataaaaaca ttttttataa atcctttgtg tcatatgaac atgcttattt
     9361 tttaatttgt taaattcgta atagcaaaat ataatctaat atttgttaaa taattatcca
     9421 aagaa
//
LOCUS       DACULQ010000147         9388 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.148, whole genome shotgun sequence.
ACCESSION   DACULQ010000147 DACULQ010000000
VERSION     DACULQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.148"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..907
                     /locus_tag="JA154_14450"
     CDS             <1..907
                     /locus_tag="JA154_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAT7997047.1"
                     /translation="KFYVAENSYLILSAPKNLKLLKETLDIDEITFNDCLKFDEITKL
                     DLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNEEHFLFEFVKNIILKNSQ
                     LEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDEMMDNINKNHVSRISDVR
                     GLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENFQGIDFGIDKLYNFALST
                     RELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITGIYGMNFRYMPELNWIYG
                     YPATLFFMLCIIFVGIIIFKIKKLL"
     gene            1084..1239
                     /locus_tag="JA154_14455"
     CDS             1084..1239
                     /locus_tag="JA154_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997048.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            1369..2382
                     /locus_tag="JA154_14460"
     CDS             1369..2382
                     /locus_tag="JA154_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAT7997049.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            2404..3468
                     /locus_tag="JA154_14465"
     CDS             2404..3468
                     /locus_tag="JA154_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAT7997050.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            3468..4781
                     /locus_tag="JA154_14470"
     CDS             3468..4781
                     /locus_tag="JA154_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAT7997051.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELNKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            4759..5838
                     /locus_tag="JA154_14475"
     CDS             4759..5838
                     /locus_tag="JA154_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAT7997052.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            5841..7037
                     /locus_tag="JA154_14480"
     CDS             5841..7037
                     /locus_tag="JA154_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAT7997053.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRFIDNDGEQKIEFSLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            7084..7893
                     /locus_tag="JA154_14485"
     CDS             7084..7893
                     /locus_tag="JA154_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAT7997054.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            7905..8438
                     /locus_tag="JA154_14490"
     CDS             7905..8438
                     /locus_tag="JA154_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAT7997055.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDINMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            8459..9298
                     /locus_tag="JA154_14495"
     CDS             8459..9298
                     /locus_tag="JA154_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HAT7997056.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3855 a          922 c         1656 g         2955 t
ORIGIN      
        1 taaattttat gtagcagaaa attcttatct tatattaagt gctccaaaaa atttaaaatt
       61 gcttaaggaa actttggata tagatgaaat aacttttaat gattgtctta aatttgatga
      121 aataaccaaa cttgatttat ttgataatta cgatttttta agcctaaata catttgaact
      181 aagagatgga gaagctgtta tagaagaggt aaatatgtat ttatcagata attttatatt
      241 ggttgtagtg aatgaagaac actttttatt tgaatttgta aaaaatatta ttttgaagaa
      301 ctctcaactt gaaaaaaatc cagttattaa tctatttaag ataaactact tgattcttag
      361 agaagttata aaaaatggtt ttgagtcttt ggaaaaagtt gaagagttaa ttcttcaaat
      421 agaagatgaa atgatggaca acattaataa gaatcatgta tctagaataa gtgatgtaag
      481 aggattaaca agaattatag ttaagaatac tagacctctt ttgtatattg gggatagaat
      541 agttaaggaa aatataagat atttaaaata ttcaaatgta aaaaaatata atttagaaaa
      601 ttttcaaggt atagattttg gaattgacaa attatacaat ttcgcattgt caacacgaga
      661 gttagcagat aaattattag atatttattc ttccagagta ggtgagaaaa caaataactt
      721 aattacaaaa ttaaccttgt taacagccat atcagcacca cttacaatta taactggtat
      781 atatggaatg aactttagat atatgccaga attaaactgg atttatggat atccagcaac
      841 tttatttttt atgctatgta taatttttgt tggaattata atatttaaaa taaaaaaatt
      901 attgtaaatt acagatgatg tttaattgat tttataaaaa tacatcaatt gagaagctga
      961 tttaagtaaa taaaaaaata ttttaaattg aaaatattta tagaaaatat tgacaattat
     1021 attttctttg ttataattat taaacaagaa gaataataaa ttataaaaat ggaggataat
     1081 actatgaaag gaatattaaa agaactaaca gcaaaacttt atagcttttt ttactttatg
     1141 ttctggggct tttattttag cgctggctat ttcttttaca aaaatatatg tacttccttt
     1201 ctggtgaggc cttattttag tgattttgag cataagtaaa ttttataaaa atgattattt
     1261 tatatattta tataagattt agtgcttgat agattatttt agagaactca ttagtgagtt
     1321 ctctttttta ttttaaatat aaaattataa tattgggagg tagtaaaaat gatagtagta
     1381 ttaaaaatgg gagcagataa aaatgaagtt aaaaaattaa ttgaggctat tgggagagaa
     1441 ggagtagaag ttaatcccat agatggaaca gaattaactg ttttaggttt agttggagat
     1501 actagtaaaa tagatgccaa aagaatagaa gcaaataagg tagttgaaaa agttatgcat
     1561 gtggtagagc cttttaaaaa ggccaataga aaatttcatc cagaaccttc aatcataaat
     1621 gttaatggaa tggaaattgg ttcaaagaaa attgctatga tagctgggcc ttgttctgtt
     1681 gaaacagaag accaaatagt atctatagct aaagatgtga aaaaatcagg tgcaggattt
     1741 ttgagaggtg gagcattcaa accaagaaca tcaccatacg ctttccaagg attgaaatat
     1801 gatggattgg atttattaaa aaaagcaaaa gaaaaaacag ggcttcctat agttacagaa
     1861 ataatgtcaa cacaagatat agatatattt gaagaaaatg tagatgtaat tcaagttgga
     1921 gctagaaaca tgcaaaactt tgatttatta aaagaattag gtaaaacaaa taaaacaata
     1981 cttttaaaaa gaggattatc tgctacaatt gaagagtggc taatgtcagc agagtatata
     2041 atggctggag gaaatgaaaa tgtagttctt tgtgaaagag gtataagaac ttttgaaaca
     2101 tatactagaa atactttgga tttaagtgca attttagctg tgaagaaact tagtcattta
     2161 ccagttatag ttgacccaag tcatgcagca ggtaaatcat ggatggttga ctctttatct
     2221 aaggcagcaa ttgcagttgg agcagatggt ctaataatcg aagttcataa tgacccagca
     2281 catgcacttt gtgatggaaa acaatcgatt aagccaaatg aatatgatga attgataagt
     2341 gaattaaaga ctatcgcatc agcagttggt agagaaatat aatctataaa aggatggtta
     2401 aacgtggaag aaaaactatt agtaaattta aaagaaaata gctacaatat atttattaaa
     2461 aaaaatatac taaagaatat aggagaagaa ataaaaaaaa tatattttgg aaataagatt
     2521 tttattgtaa ctgatgaaaa tatagcaaaa ttatacttgg ataaagtaaa aaataattta
     2581 agtgaatctg gatttgaaat taaatcttta atattaaaat ctggtgaaga aacaaaatca
     2641 tttaatactc ttcctaaaat atacgatagt ttacttgact ttaagttaac tagaaaagat
     2701 ttgattatta ctttaggagg aggggtaata ggtgatttag gtggatttgc agcagcaact
     2761 tttttaagag gtataagttt tattcaaatt ccaacatctt tattagcaca agttgatagt
     2821 tctgtaggtg gaaaagttgg agttgacctt gagagaggca aaaacttagt gggaagcttt
     2881 taccaaccaa aggctgtgtt tatcgaccca aatgtattat tatcacttcc agaaaaattc
     2941 tatagagatg gaatggcaga agttataaaa tatggatgta taaaagacaa aacttttttc
     3001 aataaacttt taagtttaaa aaatagagaa gaagtaatgc aacatataga atacattata
     3061 cataaatgtt gctttattaa aaaaactgta gttgaagctg atgaaaaaga ctttggagat
     3121 agaatgcttc taaactttgg ccatacatta ggtcatgcta ttgagaaata ctacaatttt
     3181 acgggttata cccatggaga agctgttgcc ataggaatgt ataagataac tttagaaagt
     3241 gaaaagatag gaattacaaa aaaagggaca tcagaactta taaaatcaat tttaatgaat
     3301 tataatttgc cttatgagat tgaattaaga gataataaat ctatagtaga tgctatatct
     3361 cttgataaaa aaaatctagg tgatacatta aaaataatac ttttaaaaga tattggagag
     3421 agtattatat ataatactac ttctaaattt tttcaggagg taagataatg ggaagtctaa
     3481 aaatatatcc aagtaaatta tctggagatg taaaaatacc accatcaaaa agtatggcac
     3541 atagggcagt tatttgttca tctctaagca atggaaaaag tagaatttct aatatagatt
     3601 tttctgatga tataatagct actataagag caatgacatc attaggagct attatagaaa
     3661 agaaagaaga tattttagaa atttcgggaa ttttttctaa agaaggtatt ttaaatagag
     3721 agaatcagtt gaatcaacct aaattgacaa tagattgtaa tgaatcaggc tctacattga
     3781 gatttttagt acctatatct ttagcatttg atggtgtaaa aaggtttata ggtagaggaa
     3841 atcttggaaa aagaccactt gatacttatt atgagatatt tgacagacag aatataaaat
     3901 atagttacaa agaaaatcag ttagatttga ttatatcagg taagcttaaa ccagatgaat
     3961 ttagagtaaa aggaaatata agttctcagt ttatcacagg tttactgttt atattgccta
     4021 cgctagagag tgattcaaag ataataatta ctactgagct agaatctaaa ggatatttag
     4081 atttaactct ttcaacgata aaagattttg gggtagaaat tataaacaat aactataaag
     4141 aatttattat aaaaggaaat caaacgtata aagcgagaga ttataaagta gaaggagatt
     4201 attctcaagg agcattttat ttatcagcag atgctatagg agaagatatt agcatattag
     4261 accttaaaga agattctctt caaggggata gtgaagtagt tgaaatttta tctaggatgg
     4321 gaatggaaat attaagagaa ggcaacaaaa taaaaggaat aacaaatggt ttaaatggta
     4381 ctttgataga tgcatctcaa tgcccagata taatacctgt attatctgta gtagcatcct
     4441 taagtatagg gaggactaca ataattaatg ctggtaggct tagaattaaa gaatgtgata
     4501 gacttcatgc aataaatgtt gagttaaata aacttggagc aaatatagag gagaaagagg
     4561 atagtttgat aatcgaagga gtaagcaagt taaatggtgg agtagaggtg tggagtcaca
     4621 aagaccatag aattgctatg acacttgcaa ttgcttcttg tagatgtgat aaaccaataa
     4681 tattaaaaga ttttgagtgt gtatcaaaat cttatccaca tttttttaaa gattttaaga
     4741 tgttaggagg tcgaattgat gagtggaata tggggaaata atttaaaagt atcaattttt
     4801 ggagaatctc atggaaatgc tataggtata aatatagatg gtcttccttc ggggattgaa
     4861 cttgaccttg ataatattga taaagaaatg aagagaagag cacctggaaa aaattctata
     4921 tctacttcaa gaaatgaaag tgatatacca gaaattctaa gtggatattt taatggaaga
     4981 actacaggaa cgccattatg tgcaatcata agaaatagtg atactcgttc aaaagattat
     5041 ggagagctta aaaacttaat gagaccagga catgcagatt tcacaggtaa tgtgagatat
     5101 tctggattta atgactatag aggtggaggt catttttcag gtaggataac agctccatta
     5161 gtattttgtg gggctatatg taaacaaatt ttatcacaaa aaggaataga gataggagca
     5221 cacataaaaa aaattaaaaa tatagaagat atgagttttg attatgtaaa tatttcaaaa
     5281 caacagttat caaatttgca aactttggaa ctacctttac ttgatttatc aaaggaagaa
     5341 gccatgaaaa atacgattat agatgcaaaa aatcaaggag attcagttgg tggtataatt
     5401 gagtgtgcag tagtaggtat taatgtaggg cttggaaatc cattttttga ctctgttgaa
     5461 tctactttat cacatttatt gttttctgtt cctgctgtaa aaggtgtaga gtttggttta
     5521 ggttttgaac ttgcagatat gtatggctct caaagtaatg atgaaatgta ttatgaagga
     5581 aatcaagtta agagcaaaac aaataacaat ggaggaatta taggagggat tactactgga
     5641 atgccaataa ttttcaaagt ggcaattaag ccaactccat caattagcag acaacaaaac
     5701 acagttaata taaaagataa aaaggacgat attttatata ttaaaggcag acatgaccct
     5761 tgtatagttc aaagggctat acctgtaata gaagcagtaa cagccattgg aatttttgat
     5821 ttgatgaaag gacgataata atggaggatt taacgagata tagaaatgag attgacaata
     5881 tagataaaga gcttattcaa ttatttgaaa agagaatgaa tacagttctt gaaattgcca
     5941 gacataaaat gaaaaataac actactatac ttcaaaaaga tagagaagaa aaagtactaa
     6001 gtagagccgt agataattta gataataaag catattcaca agaaacggta cagtttttta
     6061 attctataat ggaaattagt agaggattac aaaaaagatt tatagataat gatggtgagc
     6121 agaaaatcga gtttagttta gattcatcaa ataagaattt tgatttgtca aatgtaaata
     6181 aatataagtc tttaaaagat gaattaaata aaaaaaatat cctagtaggc tttcctggaa
     6241 aatcaggttc atttacagaa gaagcactaa ataaattctt caacaaaaaa atatcaaaaa
     6301 aacaatttaa agaatttgaa gatgttttca tagcacttaa aaataaagaa ataaactatg
     6361 gaataatccc catagaaaat tcatctacag gagctatctc tgaaacttat gaccttttga
     6421 gaaagtacgg tttttatata gttggagaag aatgtataaa aatagaccaa aatttaatag
     6481 ggataaaaga cacaaagctt gaagatataa aagaaatata ttctcatcca caaggtatag
     6541 gacaatgtag tgaattttta aaacaaaata gtacatggaa attgataccc tttaataaca
     6601 cagcaacaag tgcagaactt gtaagaaaac ttcaagataa aacaaaagca gcaattgcaa
     6661 gcaaaaaagc tgcaagtatt tatggattag aaataataag tccttgtata aatgatatag
     6721 caaacaacta tacaagattt gtagtaatca gtaatcaaat acacattgaa gaagaatcaa
     6781 acaaaatgag tgtagtattt tctgttgaac atgaagcagg aaaactttat aaagtacttg
     6841 gatactttgc taaaaataat ataaatatga caaaaataga atcaagacct atgaaaaatg
     6901 cctcatggag atatttcttt tatatagatt ttgagtgttc tatatacaat gctaaagtgt
     6961 acgatttatt agaattgata gaacataata cagcatattt taaatttatg ggagtatata
     7021 aaaataaaac tgaatagata agataaattt ttatttaatt tataattatt aggaggtgta
     7081 agtatgaatt ttttcggtct agtaggtgaa aaactctctc acagtgtttc acctcaaatc
     7141 cacaagagag tattcgagat attgaatatt gaaagtgctt ataaaaactt tgaaatttca
     7201 aaagaagata tatctaagct agacggtgct ataaaattat taggaataca aggggtaaat
     7261 gttactgtac catataagga gcgtattatg aaatatcttg attttatatc accagaggca
     7321 aaacgtatag gagcagttaa tacaatttta ctcagagaaa atatgttata tggttacaat
     7381 acagattact ttggtcttga tagtatgttt aaaatggcta atattgatgt tcaaggaaaa
     7441 gtagcagtta tattaggaac tggaggtgct tctaaagctg ctcttacata ttttattgat
     7501 tctggtatag aaaagcttta tgtttcaact agaaaaaagg atgataaaaa attattaaat
     7561 agtaaagcta tattaataga ttatgaagaa cttaaacata taaaaggaga tataatatta
     7621 aatgctacac cagtaggtat gtaccctaat gtaggaattt caccagtgtc aaaatctatt
     7681 atacaaaatt ttgatatatt aatagattta atttacaatc ctggagaaac agagttttta
     7741 agaattggta atagtatggg taaaaaaact tgtgatggat tatatatgct ggtggggcaa
     7801 gcaataaaat ctcaggaaat ttggcaagat actaaaatag ataatagtat tttagatgtt
     7861 atatataatg aactaaaatt agaatttttg taagggagat tgttatgata aataaaacaa
     7921 aagaaaaatt gatattaata ggtatgccag gtagtggaaa aactacaatt ggtaaacttt
     7981 tggccaaaga atataattgt agtttctgtg atatggatga ttatataatt caaatatctc
     8041 aaaaaagtat agcagagttg ttttcagaag gagaagatat ctttaggaac tatgagactc
     8101 aagcatgtag agaactttct ataagtgata aaacagtgat atctacaggt ggaggtgtaa
     8161 taaaaaagga tattaatatg gaaatactaa aagaaacagg tataataatt tttattgata
     8221 gacctataca aaagattttg gaagatataa atattaattc aagaccatta ttaaaaaacg
     8281 gaaaagatag attgtataat ctttacaatg agagaataaa tctttataaa aaattttcag
     8341 atatagagat tttaaatgat aaatcattaa ataatgctgt atataatatt acaaatgcag
     8401 tcagtgaaaa ttttaaattt gactttaaag aaaaataatc tagggggaaa taaaaataat
     8461 gaatattgtt attgtaggac taggagttat aggaggctct tttgctaaag cattaaaaaa
     8521 agctggatat gaaaatgtat ttggagtaga tgtagattta gaaactttaa aaaaagcaga
     8581 aaaagcaaaa atcataaaaa aaggatgtac tacaggtaaa gagttattta aaaaagctga
     8641 tttaattata ttatctatat atcctagatt agttgtagat tttctaaata ataataaaaa
     8701 cttttttaaa aaagggacta taattactga tactacagga ataaaagaaa cacttatcaa
     8761 tgatgtgttg caaattattc cagatgatat agattttata tttggacatc caatggcagg
     8821 tagggaaaaa aagggtattg attttgcaag tgaacaagtt tttaatggcg caaattatat
     8881 aattactcca actggcagaa acaacataaa aaatttagag ttagttgaaa atttaatttt
     8941 agagattgga tttaaaagag taaaaaagtt aacttcacaa aaacatgatg agattatagc
     9001 ttttacttct cagttacctc atgttatggc agttgctctt ataaatagtg atgaagaagg
     9061 tagagatacg ggtaaattta ttggagatag ttatagagat ttaactagaa ttgcaaatat
     9121 gaatgaagac ttatggagtg aacttttttt aggaaataga gataatttac taaaagttat
     9181 agaaaacttt gaatcagaag taaatttagt aaaagaagct atatttaaca atgataaaaa
     9241 taaattaata gagtatttta aaaaatcatc aataagaaga gaagctttag aaaaataaat
     9301 attaatattt aagctatata tagatgggta taaatataag cgatagtgta tttaattgta
     9361 ttaataaata tggattcaat ataattaa
//
LOCUS       DACULQ010000148         9263 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.149, whole genome shotgun sequence.
ACCESSION   DACULQ010000148 DACULQ010000000
VERSION     DACULQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.149"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..546)
                     /locus_tag="JA154_14500"
     CDS             complement(<1..546)
                     /locus_tag="JA154_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAT7997057.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLL"
     gene            complement(618..971)
                     /locus_tag="JA154_14505"
     CDS             complement(618..971)
                     /locus_tag="JA154_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAT7997058.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1049..1252)
                     /locus_tag="JA154_14510"
     CDS             complement(1049..1252)
                     /locus_tag="JA154_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAT7997059.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            complement(1642..1872)
                     /locus_tag="JA154_14515"
     CDS             complement(1642..1872)
                     /locus_tag="JA154_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT7997060.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1874..2393)
                     /locus_tag="JA154_14520"
                     /pseudo
     CDS             complement(1874..2393)
                     /locus_tag="JA154_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088737.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(2641..3090)
                     /locus_tag="JA154_14525"
     CDS             complement(2641..3090)
                     /locus_tag="JA154_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAT7997061.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            complement(3104..4030)
                     /locus_tag="JA154_14530"
     CDS             complement(3104..4030)
                     /locus_tag="JA154_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997062.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4155..4559)
                     /locus_tag="JA154_14535"
                     /pseudo
     CDS             complement(4155..4559)
                     /locus_tag="JA154_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            complement(4709..5329)
                     /locus_tag="JA154_14540"
     CDS             complement(4709..5329)
                     /locus_tag="JA154_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAT7997063.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5596..6696)
                     /locus_tag="JA154_14545"
                     /pseudo
     CDS             complement(5596..6696)
                     /locus_tag="JA154_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003446438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            6788..7603
                     /locus_tag="JA154_14550"
     CDS             6788..7603
                     /locus_tag="JA154_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT7997064.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSSRAEMERL"
     gene            7679..7861
                     /locus_tag="JA154_14555"
     CDS             7679..7861
                     /locus_tag="JA154_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997065.1"
                     /translation="MQSIFQKENADTPCRYRIYRHLPTYEKIIKFNISFSNKKIPNSF
                     SRIYITLLKLGWGINN"
     gene            8275..9042
                     /locus_tag="JA154_14560"
     CDS             8275..9042
                     /locus_tag="JA154_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate dehydratase"
                     /protein_id="HAT7997066.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
BASE COUNT         3087 a         1507 c         1431 g         3238 t
ORIGIN      
        1 taataatttt gaaattgtat cattattaaa tctctttttt ataatttttg caggtactcc
       61 tccaacaata gaatacggtg gaacgtcatt agtaactact gctcttgttc caataattgc
      121 accatcacca atatttacac ctgacataat tatagaatca taacctatcc acacatcatt
      181 tccaattata atgttaccct tattgtccca agcgtccgtt atatggttga tatcgagttt
      241 ccattcttca taaaagatgg gaaaagtata tgtggatttt gatttcattg agtgatttcc
      301 actagtcatt agaaattttg cattacatgc tattgagcaa aacttaccaa ttattaattt
      361 atcattgttt atagggtaat gatacaatac attgtttttc tcaaaatctc ttgggtcatt
      421 ataaaagtca ttatatattg tgtaatctcc aacttctata ttttcccttg ttattacatt
      481 tttaagatat acagtttgat aatcattact tcttggatat accttatttg aacttggaat
      541 agtcatattt aaatctctcc tttaatatat attatgtttt tgactattcc actactacaa
      601 tacatctctt cacagaatca ctccttgtta agaatcttca taagataatg gcgtcctttt
      661 ttagtgttaa gtcggatatt ctttcttagt ccattctttt tagcgtataa ctgacaaact
      721 gtcattttct taccaatagt gtcaatgcta tcatcaaggt cattttgtat tgctttttcc
      781 ttatccctta atgatatatc atcacgtttt ccctttgggg gtttatcagt aggtacaagt
      841 ttccaagagt aaacaaattg tggtttacca aatgaatata catacttata tgcgtatctt
      901 ccatcttttc tctggctctc tccagttctt aaaattctat ctttattatc tcgtcttttt
      961 tcagttgaca tgataagaag ctcctctcaa taaccgaatt gagcttgata cgtcatatga
     1021 atattataac atataaggct caatttcatt agataaagtc aagtgtatca acgattttct
     1081 caaattgttt tctctttatt tgaatgcgat tgctattcat aatgacccag ccagaatcaa
     1141 ggttttcatc agcaagttta cgtaattttt tctctccaat acgaaagtat tttgacgctt
     1201 cttcaatcgt caaggtatat ttttcccaaa taggtacatc agtattgttc atagaatgta
     1261 tcactccttt ctatgttcgt agttcagaag tagatagatg gctttggtaa gctccattgg
     1321 tattttaacc aatcccattc ttatgggcag aaccgtgtca tacaggtgta tcattatact
     1381 gtggtacagg ttacggcaaa acaaccgttc caaaggttat tcacaaatca ctatatagta
     1441 gctctcttga ttaaggtcat ggcgtatagt atttgttggc tatctgtatc acaaacgtat
     1501 ctatctactt tattcagttg tcaaagaaca gtcggcattg atttgtctat aaaaaatcac
     1561 aatagcttat gggcttttat agacaaataa ttagtgattc tccatgtacc aaatagcttg
     1621 atacatggag agagggcgag attaagacaa attaaatctc aaaatagcag tgattagttt
     1681 tgttcctagc attcgctttg tttcttcttc tacatgaata tagggatttc cgtattcatc
     1741 aaataaagtt ttctgtgaaa gacgaatgat atagtgttca aaatgtttaa gtattccgtt
     1801 tattgcattt acatcgccat gtacagcaag taaaataatt gtaaaaggta atacttcttt
     1861 agtaggtttc atttcaatgt tcctccatga gttctttcaa ctcttttagt gcatttgttt
     1921 tgtgataatg tatggtattt tctgctaaat ccatcaatgt tgctatgtca gcatttgtca
     1981 tgttgagaaa gaatgaaagt agaattatat cttgttttct ttttgtaagt tttccgattg
     2041 cttgtgccat taatacatct tcaattcgta tgttgtagtc gtaagaaacc atacagatgt
     2101 agttagaatc ataatcatct aaaatactca atttacttaa ttctgcaagt gacaaagatt
     2161 cgagagatac caaataatca tttctgcgtt tattttcgtc ataaatatct ctagcatagt
     2221 tacgcaaaac tgttttacag aaactgtcga attgatgttc aattgtcttt tcatcaacca
     2281 taagatttca ccctctttcc atcgcaagga gttaaataac cttgcttact cctatcacgg
     2341 tgggagggcg ttaaatcttc gataaatttc aaaaaaaaga gaaaaaaata cacactctgc
     2401 ctatagggca aaaatgtgta ttctttaaaa cgtatttaat ttatattggt tgtaagccac
     2461 caccatattg atgtatggaa atatgcattg gctagctttt cataaaaata caaagatgca
     2521 taagaaatta atagagggga aaaacttatt ctgttattaa aaagtaggtt aattctaata
     2581 aatgctattg cggtcgaatt aaactatata catggcaatc aatcatgttt ctattcttta
     2641 ttatgaattg tgacagtatt cttcaatctt aaaaccacat ttttccaata ctttacttga
     2701 ccctctattg taggaatata cattagcatg aattttgtta aggttgtatt tttcaaaggc
     2761 aattttgcat atttctttta ctgcatttgt tgctatccct ttgccccaat agttcctagc
     2821 aagccagtaa cctaattcag catttttatt aatagaggta tcttcttcaa atgttaggct
     2881 gatacttcca atagcccttt cttgataaaa aatagcatag ctaagttgtt taacatctgg
     2941 agttgccaaa caaaaactaa caaaatctct agcgttttgc tccctataag ggtgggggaa
     3001 gttgtcacga agattctttg caatttcttt atcattcgca aactcaacta aatcggtaat
     3061 ataagcataa ttccactcta taagttccat ttttcttctc cttttatgaa acagctacaa
     3121 gaaatctttc taagccaata ccacttatca aacagcgttc ttttttattt ccaagcattt
     3181 gataagtcca gtcaacaaat ccaccgtcaa ctatttcaac ggtttcattt ccttgtttga
     3241 ccgataattt gaaattgata cctttataat aattattatc aacatcatca ttatgaattg
     3301 ataactctat attaggaaaa ataagtttga tagtttcaac cattctgtcg aaaaatccgt
     3361 cattatcttt gtatccgttt cttttgcgta gaataataga gaaacgaaca ttttctattt
     3421 cacttaatat atctttataa tagtttaagt gcttttctaa caacattttt tcacaagtgt
     3481 aagaccctgt atctatacca gatgaaacca tacaatataa gccaaaatgt gaatataaac
     3541 ctttacctac aaaattttgt gctcttatta ccctagcagt agtagccatg tggataggta
     3601 tggtattatt ttcttctttt ctttttaatt tatcagcaat aattattgcc aacatatttg
     3661 aggggtctgc aagtgtttct gtccctcgta gagcacttac tacattatat tgatctacgg
     3721 agccaaaaac gctacagcta cctaatggag cagagggaga aagcataatc gtcttgatat
     3781 ccatattgaa tgcttttctc aataattttg actccaaagc atgaatttct tctggatcta
     3841 taccgctagg gatagtaaat ctatttagtt ggtatgactt tagaatatca gttgcagtta
     3901 aagtatttgc ttgtctatca aacacttcta atagaagtga gttcaaatct gattttgata
     3961 gagctgataa cttatcaact atattcttat ccccaatttt atttgtaatt ctacttaaaa
     4021 tatcactcat agttatcctc catactgtta ctttcaaaat aattatagaa tggttacaac
     4081 aactaatcta ggtcatttga ccaaaaatga aaaaacactg aatatttact attcaatatt
     4141 tttttacttt gggtttattt gattaaatct tgaaaatact gtaatgcaac cctttcagct
     4201 tcaacctttc ggacaatagg actttgtgat aattcctctc ttaactcaag atacaggttg
     4261 atagtagcct gcaatgcatt aacacagctt ttatattcat ggttagcaac cgtcttttct
     4321 aatgcttcaa tatattgggg tgcgtccaat tcaatttttc taactccacg aggtaattta
     4381 cctaagagaa gctgtattaa tggggcaagt actgattgtc ttaaaaaaga taaaaaatca
     4441 attgtttcaa atagttctct acgtccaatc ttagtgcaag cgtaatgaac ccaaatccaa
     4501 aatctatctt caatccactg taagtctgga gttgggaagt atgcttcttt tttagtataa
     4561 cacctctcac tttcttttat tagctatcaa tccaacataa ttatgcatag gagtattata
     4621 taatgaaaaa ataaaaaaaa ataggtcact tgacccgaaa taaaaaagca ttgaataatt
     4681 attattcaat acttttttga aattatactt atgttgtagg aagattcttt aatacctctc
     4741 gctgattaat tacactaaat cttttgtttt cataagcaat tagatgttct tgtacacatt
     4801 catttatagc acgattaatg cttctaattg gagcgtttac ttcgctagca agctgttttt
     4861 tggtaagcag atttaattca cttctataat agtgtaagga tatacacctc agaagtcgtt
     4921 ctttaacact taaagatgat aattcttcaa ttaattcagc atttacaact aatttactag
     4981 atatttctcg aatcagaaat tttgtagctt caaaatctcc ccttaaccag tttaaaaaat
     5041 catctctatg aagtttatat acattacaat ctgtgaaagc gacaatttca acgggttttt
     5101 tcccatcatt aaattgttcc atttctccaa aaaaactacc acttctataa agatgaatag
     5161 ttgagatgtt tcctttcaca tttaagatga atgcttctgc aataccgtca atcataagaa
     5221 ttacatattc attttcttgc tcagcaaaaa gaatagtctc tttcttttta tacgtttttt
     5281 taacaagctt ctttttaata tcttcattca taaaatcaat taaatgcata ttcatccctc
     5341 cttaccaaat ctttgattaa attatatcat ctttcaatta gttaggtaag atagtaaaaa
     5401 tatatttaca tagaaactat tccattatta ttttccagtt gtcatttttc tgcaaagtaa
     5461 gctcaaactg agaaagctgt tgacaataga actagtagga atatatgagc gaaccatttc
     5521 agtattcaac tgttgtaggt tttcagtcag ataattcttc aaacgttcaa ttcgtttgtc
     5581 ctgtgaatct tgcgatgctc taaccagttt catagatagc gttttacctt gtgccacctg
     5641 caaaaaagac catgcttcgt caaggtcaac tatcttgaag ctagagcggt cagtatgaat
     5701 aaagtctaat gcaaaggtac taataacaat caacatagca acactaagaa gttctattgt
     5761 tgtatattct tcaaagctag tgttggcatc tggcaatacg aggtcagcaa cttgaataat
     5821 attcagctgt ttctccaagc tgatagactg cttcacattt ccgtcaccaa acagtagatg
     5881 tgcaaaatca tagtctacaa aactttcgat atggtcggct atgctgttac tgatagaagt
     5941 atcttctttt cgtaattcat caatgacaag taataaccct cgctgttcac tttgggttac
     6001 attgcggatt gcttttctaa gcactgggaa cttatcactg tcacgactgg agataccagc
     6061 aaggaatgtt aggatatcaa tcgctaaact ttcagaatca tttttacggc tcaaaatgac
     6121 gtaagggtca agcaatcctt tgttttcatc attactggtt aaattgacaa tgtttatctc
     6181 atgggctatt tctggtagcg tttctttcca tttgccgcgt tctgctttag ggtcaactat
     6241 gaccgcctgt ccgccgaata gtaccgcata gtaaacaagc atattgttac tgaataattt
     6301 accaccaccc aatgaaccga taaacgcaga cgctaacaca ttggtaatag aaccctttac
     6361 ccattgacta gcaagggcag gttttagata gacattcttt ccagtgtcca gattgtagcc
     6421 aagatagata tcctcatgtt cgccaagggt ttgtgtcgct ccaaaaccaa gtccagccaa
     6481 aaaatccgaa gtgacatatt gaatataatc attgatgtaa cgtttgctga caggaataaa
     6541 ttcgccatgc aatcccaaca tatccccaaa gggacgcacg agcttgagtg ataagtcgtc
     6601 gtaaaagtct ttgacttcat cacaacgcct ttttagctcg tcaagatcct ttgtagatac
     6661 acggacaaca tagctaagtt tatacataga ttctttacag ttaaaagtct gtttttaaaa
     6721 aaatgtataa attataaatc aaaatataga tactatcaat gacccactag gtgcaaaaag
     6781 cattgatatg gttaaattat cagacgctaa aagctgggct atccgaatga gtgaagatgg
     6841 gtgtgcttat aaatccattg acaattataa gcgctcactg aaagcgtcgt ttttcatggc
     6901 aattcaagac gactgtatta gaaagaatcc atttaatttt cagttaagtg atgttttgga
     6961 agatacaaca gaaccaaaaa tcgctctaac accagaacag gaaatgggct tattatcctt
     7021 tatggagagc gataagactt attgtaaata ttatgatgat gttgttatcc tcttagaaac
     7081 aggactacgc atttctgaat tatgcggttt gactttagaa ttggatatga aaaataaagc
     7141 cattatggta gaccatcaat tgttgagtga tactaagatt ggctattatg tagaagttcc
     7201 aaagaccaca cagggtaagc gagaaattcc aatgactgaa agagcttatc aatcatttga
     7261 acgtatcgtg gaacggcgta agaacgctaa agcagaacca atcattgttg atggttacca
     7321 gaacttccta tttcttaaac aagatggatt accaaaacaa gcaactcatt atcaaagcat
     7381 gttaagagcg ttagtcaaga agtataataa gacccatgag gacaagctac caaagattac
     7441 gccacatacc tttaggcata cactctgtac aaatatggct aacaaaggaa tgagtcctaa
     7501 tatattgcaa tatattatgg ggcataaaaa cattactatg acgcttggct attatgcaca
     7561 tggttcattt agttcttcga gagctgaaat ggaacggtta taatcctagt agtatgacta
     7621 gagtttacta cttatttact acttttgatg gcattcttat acggttaaat gccaacttat
     7681 gcaaagtatc ttccaaaaag aaaatgccga tactccttgt agatacagga tatatcgaca
     7741 tttaccaact tatgaaaaga taatcaaatt taatataagt ttttcaaata aaaagatacc
     7801 aaattctttt tcgagaatct atataacatt acttaagctt ggttggggta taaacaattg
     7861 agtagctact tttgtatggt gttcaacttt agtcaaaact cttaagtaat gcaaatgatt
     7921 tagattcatt aatattcaat ctcttcataa gtagtgaaat tcgagttatt tctgcataat
     7981 tatactatga tttatagatt aattctatta gcatccagaa taatattgtt tatatgagta
     8041 aaatttaaag aatattgtga aaattatagg atgttaaaat aaatttaggt aaaaaagaag
     8101 tgtttgtata ttatagagta ccttttctaa aatatgtggg attgttttaa gaattagaat
     8161 aaaaattatt tttaaattag tatttttata atgtatagta tttttaatgt ataattaaat
     8221 tgtgcagaaa atagaattta aagtcctata gaatttctga ttatggagga gaatatgaaa
     8281 agaaaagttc aagtaaaaaa tattactatt ggagaaggta gacctaaaat ttgtgtacca
     8341 attattggga aaaataaaaa agatattata aaagaggcta aagagttaaa agatgcatgt
     8401 ttggatatta tagagtggcg agttgatttt tttgagaatg tagaaaatat taaagaagtt
     8461 aaggaagttc tctatgaatt aagaagttat atacatgata tccctttatt attcacattt
     8521 agaagtgtag tagaaggggg agaaaaatta atatcaagag attattatac tactttgaat
     8581 aaagaaattt ctaatacagg tttagtagat ttaatagatg tagagttatt tatgggagat
     8641 gaggtcatag atgaagtagt taattttgct cataaaaaag aagtgaaagt aattatatct
     8701 aatcatgatt ttaataaaac tccaaaaaaa gaagagattg tatctcgttt atgtagaatg
     8761 caggaattgg gcgcagattt acccaaaata gctgttatgc cgcaaaatga aaaagatgtg
     8821 ttagtgctac ttgaagctac aaatgaaatg tttaaaattt atgcagatag accaataata
     8881 acaatgtcaa tgtcaggaat gggtgttata agtagattgt gtggtgaaat atttggttct
     8941 gcattgactt ttggagctgc aaaaagtgtt tcagcaccag ggcaaatatc atttaaagag
     9001 ttaaattcag tacttaatct attacataag agtattaatt aaattaatta tatagaaata
     9061 ttagacggta tttttgaaga tataaattaa atataaaaaa cttttcagat agtaaaattt
     9121 aaattgggca attatatctg aaaagcaact atttagtgtg tattttgaaa gtgatataga
     9181 aaatcttata aatcaactat ggcatattta aaatatgcca ttaaaaccta ctagacatta
     9241 gattatagat ggtatactag cat
//
LOCUS       DACULQ010000149         9255 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.150, whole genome shotgun sequence.
ACCESSION   DACULQ010000149 DACULQ010000000
VERSION     DACULQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.150"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            15..644
                     /locus_tag="JA154_14565"
     CDS             15..644
                     /locus_tag="JA154_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997067.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIITECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            645..1427
                     /locus_tag="JA154_14570"
     CDS             645..1427
                     /locus_tag="JA154_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997068.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1420..2298
                     /locus_tag="JA154_14575"
     CDS             1420..2298
                     /locus_tag="JA154_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997069.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNKEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIRSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2331..3290
                     /locus_tag="JA154_14580"
     CDS             2331..3290
                     /locus_tag="JA154_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7997070.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3604..4296
                     /locus_tag="JA154_14585"
     CDS             3604..4296
                     /locus_tag="JA154_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7997071.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4306..5310
                     /locus_tag="JA154_14590"
     CDS             4306..5310
                     /locus_tag="JA154_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7997072.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFAP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKGVNSLIKM
                     "
     gene            5555..6409
                     /locus_tag="JA154_14595"
     CDS             5555..6409
                     /locus_tag="JA154_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase"
                     /protein_id="HAT7997073.1"
                     /translation="MMSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKG
                     QFAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGC
                     IPFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQ
                     ALKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFE
                     FDYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVL
                     AFTNIISV"
     gene            6513..7259
                     /locus_tag="JA154_14600"
     CDS             6513..7259
                     /locus_tag="JA154_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacitracin ABC transporter ATP-binding protein"
                     /protein_id="HAT7997074.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7259..9175
                     /locus_tag="JA154_14605"
     CDS             7259..9175
                     /locus_tag="JA154_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997075.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
BASE COUNT         3577 a          872 c         1663 g         3143 t
ORIGIN      
        1 taataggagg cgttatgaat aaatatcttc taataaatcc agtggcagaa agaatgtatg
       61 gagaaaagtt tcaccttatt aaagcaaatt tgataaaaaa ggggtacata ataacagaat
      121 gtgaaccaca gttagaatat gtaaaaaagc agtacaaaga gtatacaaaa aagactgaaa
      181 atacaatgtt ggattgtaga tgtccagaaa gtataagtct tttaaaaaga aataatttga
      241 taaataattt tgaagtacct atgatagaac caatattagt aaggacttgt agggttttat
      301 acgataaata tataaaaagt aaagatgata tgttggtagt aacctgtcca tgtacacaat
      361 taagagactt tgcaagtgaa aagtttaagg ataagggtaa tgctatgttt tatacatgga
      421 aagagtttgc tgaacaagaa ggtgtaaaat cacttggaaa aatagatgaa tcaccaattc
      481 cattaggata tttcaaagat acttttgaaa aagttcttga agtatcaagt gaagacgaaa
      541 tattaagtga aatacaaaaa ataagaaatg gttttgaaga taagtatgac atagtagaaa
      601 tgttttattg taaagatggt tgtaataatg gagatggttt gtaaatggtc aataaattta
      661 ataataagtt tttaaggcta gtaaagagtg ttctgatatt ttttataatt cttttattat
      721 ggaaaattac aaattatctt ggtatttgga gtgattatat attaccatca cctgaaaagg
      781 tatatagtac atttttaaat atgataagtg atggttcaat atttattaat gtatatgcaa
      841 gtatgaaaag agttttgata ggttttgcaa taagtactgc tataggtgtg cctttaggga
      901 tattctttgg aatttatagt ggagtatacg aatactttaa atctctaata aattttttaa
      961 ggaatacacc tccccttgct ttaataccaa tgcttatatt atggtttggt ataggagagg
     1021 aatcaaaaat tataattata gttttagcat cttttttccc aatctttaca agcacattga
     1081 aagggattaa aaattgtgac tcaaaactga ttgaagtggg tagagtattt gagttttcaa
     1141 agcttcagat aatatttaaa ataataatac caaatgccat tcttgatatt gctgttgggt
     1201 taaagttagc tcttggatat agctttagag caattattgg ggcagaactt gtagcagctt
     1261 catctggtct tggatacttg atatctgatg gaaaagaaat gtctagaaca gatgttgtta
     1321 ttgtagggat aatcgtaatt ggacttttag gaataattac agattacata ttttcgatta
     1381 ttgtgaagaa ggtaagtaag ggaaagatgg tggaagcata tgaataattt agtatgtgac
     1441 tctggatata taattagaaa tttatataaa agttataaag ttgacaataa agaacatgta
     1501 gttcttagtg atatctcttt aaacatagat agaaaccata taacagtaat tttaggtgaa
     1561 agtggttgtg gaaagactac cttgcttaga gcaattgcat cacttgaagg tataaatagt
     1621 ggtgaaatta ggtttattaa tgataataaa gagtatagac ctaatgttgg ttttgttttt
     1681 caggagagta gattaatgcc ttggttaaac gtaagtgaaa atatagcttt tcataatcaa
     1741 agaaaaaata ctattttaaa caaggtgctt agaaaattta aacacaataa aagaattagg
     1801 agtaaaatta taaatgataa ttttagaagc ttagaaacaa atgataattt gaacaaatca
     1861 aataaatata ataggaaaag taatattgat gtagaaaaat atcttaagat gatgaacttg
     1921 cagaaattta aaaactctta tccaaatgaa ttgtcaggag gtatggcgca gagagtaagc
     1981 atagctagag ctctttcttt caatccagat atgttgctta tggacgagcc tttttcggca
     2041 ttagattatt ttacaaggat tgatatgcaa aatgaagtta taagaataca taagtcaact
     2101 aataaaggcg tgatatttgt aactcatgat attgatgaag cgttaaagat aggcaagaaa
     2161 atagttgttt tcacagacga aagaaaagta aaagaattta atatagaaga tggttataat
     2221 agagatttaa cttcagatta ctatattaaa ttaaaaaaag atattttaat gacaatgaag
     2281 tcaaataaag gcatttaaag aaattaataa tattttaagg agtaaagtaa atgaaattaa
     2341 aaaagctatt gataggtata acgatattaa cacttgcaac agcatcttta gctggatgtg
     2401 ctaaaaaaaa taatggagaa aaactttcag aaataaattt aacttatgta aaatcaccat
     2461 taaatgttcc ttctataatt cagaaacagg atgatttatt tggaaaggaa tttaaaaagg
     2521 ataatataaa agttaacttc cacgaaatta ctacaggtcc agagcaaaca caagctttag
     2581 ctgctggaga aatagacttc ttacatgcac taggtggtac atcagcttta atagctgcat
     2641 ctaatggagt agagttaaag atattaaaca cttatagcag gtcaccaaaa ggatttatga
     2701 ttttgactaa taataattca ataaaatcag cagcagattt agtaggtaag aaggtggcag
     2761 gcccaaaagg gactatatta catcaggtat tgatttcagc acttgataaa gaaggattaa
     2821 gtatggatga tgtggaattt gttaatatgg gtattccaga ggcatcagca gctctttctg
     2881 atggcagcgt agatgctgca ttgattgctg ggccagcagc tcttaaagct atgaagtcag
     2941 gtagtaaact tgtagcaaat ggtgaaggtt tagtagatgg tataatagta actgctgtta
     3001 gcacagactt tgcagagaaa catccagaaa tagttgaaag atttatgaag gttgaaaagg
     3061 aaactttaga atatgtaaat aataattttg atgaagctat ggaaaaagta gctaaagaag
     3121 ttgatttaag tcttgaagaa actaaagaat tatatgcttg gtatgatttt agtttagata
     3181 taacagataa agacatatct tcgcttgaag atacacaaga cttcttaata aaaaataagt
     3241 tacaagagaa aaaagtgaac ataaaagaat taatctataa tgctaagtag aaactatgat
     3301 aaaataatac taagtaagaa atataataaa agtttaagtt accttattaa ggtaactttt
     3361 tctattttga attagtaatt tcaatttatt ttagaatgat tagttgataa taatgattaa
     3421 ttaatcataa aaagatactt ttttatagag ttttattaag atgtctaata atatggttat
     3481 acttatgaag tcagtaattg tgaagttagt aatatgatta aatttatata ttgtaaaaag
     3541 attataatat attaaaattg gactgttata tgatgagtaa ataggtatat taggaggaat
     3601 tatatgagtt ataaagtata tattgtggaa gatgacatat caataagtac attacttaaa
     3661 gattatattg agaaatatgg atttgaagtg attgttgaag agaactttga tgatataatg
     3721 attacatttg aaagttttaa gccacactta gttctattag atgtaaattt acctaaattt
     3781 gatggtttct actggtgtag atgtattagg caacagtcga atttacctat aatttttata
     3841 tcagctagag atggtaatgt agaccaaatt agagctttag aaagtggagc agatgattat
     3901 ataacaaaac ctttttacta tgatgtagta atggcaaaaa taaagagtca tataagaagg
     3961 gtatatggtg aatattcacc taaaatagac gaaagaatag tggagctaga tggattaaaa
     4021 ttttatccag aaaggttaga actagaatta aatggtgaga atctaattat aactaaaaaa
     4081 gaaggtatac tgattgaatg tcttatcaag agatacccaa aagtagtaag tagagattat
     4141 cttttagaaa agatatggga tgatattgag tttgtagagg aaaatacttt aaatgtaaat
     4201 gttagcagga taaggaaaag atttgaggaa ttaggtatag ataatgctgt tcagactgtc
     4261 cgagggttag gatataaatt aaataagaat tggtaaggag aaagtatgaa gttattttta
     4321 aaagattata aaggttatat ttttatatat tatataggca tagttatgac aattatttac
     4381 tgtaatctta tgaagtttat tagttttgga gagagtttat atattctttt atttagttct
     4441 tttatattgt tatgtttttt aatatataag tattataaaa atagagaagt atataatctg
     4501 tttgaaaaag gagtacattc tttggaggag tcatttttgt atttaggaag ctcattcttg
     4561 gggagaaata tatcagttat tttaaaacaa caacataaat tgtatcaatc aatgatacaa
     4621 gagcataaaa agatacatag tgaacattta actttcatca accaatggat tcatcagatg
     4681 aaaacaccaa tatcagttat aagccttcag ctccaagatt atgagggaga agaagtaact
     4741 gaaaatataa gaaaagaaat agatagattg aataaagggt tagatatggc tatgcatttt
     4801 gcaagggcag ataattttca gaaagacttt atagttgaaa aagccaaatt aaatgaaatt
     4861 gtatctagta ttgtaaatga agaaaaaagg ttatttataa aaagtggaat gattcctaag
     4921 attcaagtag acaagtttac ttatgtttat acagatgtaa aatggatgaa atttataata
     4981 ggtcaattaa ttacaaatgg agtgaaatat tctaaagatt atagtaacta cctaactata
     5041 aaatctgaag aaaataataa atatgtaatt ttaagtataa tagatgaagg aattggaatt
     5101 gtttctaaag atttaaaaag ggtatttgcc ccatttttta caggagaaaa tggtcgtaag
     5161 tttggagaat ctacaggtat gggtctttat ctagtaaaaa aggtatgtga tgatttaggt
     5221 cataaagtaa gtataaaatc agaagtagga aaaggtacag aagtttcaat ctactttaat
     5281 aaaggtgtaa atagtttgat aaagatgtaa attatctgtt acaatagggt ttttagttaa
     5341 ttgtactttt ttattataca atttattaaa tgaaatattc atagttaaat aatcaaaatt
     5401 taagagagtt tatttgcaaa ccttacatag atgtaaggtt tgtgtaatgt ttgcagatag
     5461 ttaaattttc taatctaaat tacaattaat ataagaaata acataagaga aataacataa
     5521 aaaaagaaat tttgtaaaaa aggaaggatg tttattgatg agtttagatg ttatttttat
     5581 attgaaatca gtcataatag ctatagtaga ggggcttaca gaatttattc cagtttcatc
     5641 tacaggtcat atgattttgg ttggtaattt aatagatttt aaggggcaat ttgctgaaat
     5701 gtttgaggtt gtaatacaac ttggggctat attagcagta gtagtacttt attggaaaaa
     5761 gataaaagat agcgttatag aattttttaa atttatattt actggtggaa aagaaggaaa
     5821 gattggtttt agatttggaa tgaatgtaat tataggttgt ataccatttg cgattatagg
     5881 agttctattt tacgataata taaaatcatt gtttaattta caatctgtaa ttataggatt
     5941 tatagtgggt gggattttat tattagtagt agaaactttg tttagaaaaa agaatcattc
     6001 cacagataat atagataaaa ttactccaat acaagcttta aaggttggga cattacaagt
     6061 tttgtctgca tggccaggta tgtcaaggag tgcatctact attatgggtg gatggatagc
     6121 aggacttaat tctcctacag cagctgagtt ttctttcttt ttagcagtac cagctatggt
     6181 ggcttcttca ggaaaagatt tatttgaatt tgattattca ataatgacac caacattgtg
     6241 gattgctttg gtagttgggt ttatagtagc ttttattgta tcaataatag ttatggaaaa
     6301 attcgtgaat tttttaaaga aaaaaccaat gagagtgttt gctgtataca gaataatcat
     6361 gggtgtagta cttgcagtat tagcttttac taacataatt agtgtataga aaaaatagat
     6421 ttattatcta taaaaattaa actaagataa attataatta acaattatat atatgttatt
     6481 tcaaaaatta aattaggaag gatagttaaa taatgaaaat tttaactgta aataatttaa
     6541 gtaaagtata tggtaaaaaa ataattttca acgcattaaa tgacataaat tttagtatag
     6601 aagatggaga atttgtagga attatgggtc catctggtag tggaaaaact actcttttaa
     6661 atatgatttc tactattgac aagccaacta caggaactat ggaattaaag ggcaagaatc
     6721 ctttattgtt aagaggtgag gaacttgcat tatttagaag aagagagtta ggttttgtat
     6781 ttcaggattt taatttattg gatacattaa ctataggaga aaatatagta ttgccattga
     6841 cattagataa ggtcagtgta aaagagcaag atgagagatt gaatgaagta tctacaatat
     6901 taggtataaa agatttatta ggaaaaagaa cttttgaagt atcaggtggt caagctcaga
     6961 ggactgctat tgctagagca ttgattaaca atccatcaat acttttagca gatgaaccaa
     7021 caggtaattt agattcaaag tcatcaaagg ttgtaatgga gctatttcaa aagataaata
     7081 aagaaaataa agttactact atgatggtaa ctcatgaccc attagcagcg agctactgta
     7141 gtagaatctt attcataaaa gatgggtcta tatataatga aatatataag ggtagtagta
     7201 gagagcagtt ttatcaagag ataatggatg tacttacatt attgggaggc gataactaat
     7261 gaatataaaa cagtttgcta ttaacaatat ttcaagaaat attaaagcgt atttaggata
     7321 cttagcaagt attgttatat catcttcact gctattttct tttattatgt ttacaagtca
     7381 tccagattta gatgtatctc aatttcctga ttatttaaaa gaaggtctca agatgagtaa
     7441 gataattgca tatctattct tattcttctt tgtgttttat tcagttagtg tgtttcttaa
     7501 aagtagatat aaagaatttg gaatacttta tattacaggt atatcaaagc gccaagtaat
     7561 gaaattaatc tttatagaaa atatgataat aaatatagta tcttctgtaa ttggaataat
     7621 aataggctta atctttgcaa agatattttt agtggttatg tctacttttt tagaattatc
     7681 accattaaaa ttctatatac catttaatgc gatattgtct actttaatct attttatgat
     7741 tttagctgta ttaacatcaa tatttacttc ttttatagtt aaagaaaatc aggtgttgaa
     7801 gttacttaaa ggtacaaaaa ctccaaaacc agagcctaaa acatcactaa tgcttgctat
     7861 attaagtatt tgtttgttta taatagcgta ttataatgct attacctcaa ccgaccaata
     7921 tacaacatat ctgcgcttag ttccagttac atcccttact atagtagcaa cttacttgtt
     7981 cttttctcaa tttagtgtat tttttatgaa gaagctaaaa ttaaacaaga gattttatag
     8041 aaaaaataca aatatgcttt ggatttcgaa cttgatttat aaggtaaagg ataatgctag
     8101 aatatttttc ttgataacta taacttctgc agtggcattt actgctatag gtacagttta
     8161 ttccttttgg aaagatgtag agcgtcaaat taatttaata tatcctaaca caatatatta
     8221 ttcaacaatg acattgcata atgaccctaa aaagcctgat agcaaagaaa aagacaagga
     8281 aagaatgatg tttatagaaa ataatctaaa aaaagagaat gtagactata caaggattaa
     8341 tggtgaattt aaaactgtat ttcccaagaa aagtgatttt aatgtcagaa ttatgaaaga
     8401 atctaagtac ttagaaatta cagaaactat aggggttaag cctataagtt ttgaagataa
     8461 tgaatgtata tcattattat catcaagttt accaggaaat aagaacgtca aagaaaatat
     8521 tattgtaggg aatagaggtt taaaggtggc aaaacaagtg gaagaatgtg ttatgccagc
     8581 gtattacaaa aatatgtatg tggtaaagga taatttttat gatagtatag aatcaaaatt
     8641 tatagtggat agatttactg catttgaagt agaggattca agtaaaacta tagagatatg
     8701 cagagaattt gaaaataaat ttaataatga gtcagggatg caaccatatt tgtttttctc
     8761 taaagaatta atgataggaa ctgggaaatt aatgtattct acatttatat ttttagcaat
     8821 atttataggt ttaattttct ttgttactac aagtagtttt ttatataata aattttatat
     8881 ggattgccaa gttgataaga aaaagtatga acagttaaat aaattgggta tgacatataa
     8941 tgagattaaa aaagcttcaa caattgagat tggaatagta tttttgtttc cttatgtagt
     9001 ggctgttata cattcaatat ttgcacttac tgcactgaaa aattcttttg atatagaagt
     9061 agcatcatca gctgttttgg taatgggaag cttgttcttg gttcaaatag tttatttttt
     9121 aattgtaaga aataattatt taaaagagat aaggttaaat ttagtcaatt tttagttact
     9181 attattaaat gataataaaa agggtttaga attaattaat attactggaa ttatagatgt
     9241 ttattttttc agtaa
//
LOCUS       DACULQ010000150         9129 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.151, whole genome shotgun sequence.
ACCESSION   DACULQ010000150 DACULQ010000000
VERSION     DACULQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.151"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..918
                     /locus_tag="JA154_14610"
     CDS             <1..918
                     /locus_tag="JA154_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997076.1"
                     /translation="KIYNLLDIKPFKPTYETNKLEAYSTDSPISDDDSMDEYISNYKF
                     DINLNEIILSLSINQGIYNSKKEHVTSFMISDNNEYIFCTKKAIAYSIDWDKKSVRKK
                     KLSEAKVNDWFLFVDDYLNEEGELVINVIQKLLELNLLPSHYLDDYKLTNSWKSELKN
                     YISYSKYNYNDVSNSLAVLGEKINPITIKNWVTNKRLIGPIYLKTFEKIFKVIDSKLD
                     HTEVYDATKSIRSLHTKIKKSIDTFVSDNFFSSKSDIIKDEVSAVVLSMLGNTNDYVT
                     PVQIREIHSCDKYLSPHLVNKVLDLYSVY"
     gene            936..4463
                     /locus_tag="JA154_14615"
     CDS             936..4463
                     /locus_tag="JA154_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAT7997077.1"
                     /translation="MNCSELRDKTLDLVRKELLGPGSEDLCENIKYEIITDSPIERYS
                     TGILYPLNNNIDVSNTSEYDGEDNVFTSDDTQNELDRAISMSNETLPSSMGMTFFLKK
                     DAEVINFRVNYATYRNALYTDCHVKYTGDDFYSSNTINEYVELRGSFLYLKQKLTSEK
                     VYNLLKKHDLLDNAEAKFVLYKLANQCPDDENKTGGYVRVPHENLIVSIDMDKNYIKK
                     PISKDLDIVLSKRIYSNDFVSITCMLVNSQSGPKKYNNTIFQPELMVLAEDNNDLEFI
                     DIFTAGSVNTFKKLDSEEISLDLLYRNKKTFATGHGVAIDQDIQNDALIKLCTEFIPH
                     FEVPKLDFNVDELSDVSRELLSMKNLSDISDLSKDKKIYFLYKFVDAYDIWIDDLEED
                     SKDLSDVFKDVAYEHIKNCRDTKSRMLEGINLIKSNDEVYRAFSLMNRAILMQRCHSI
                     HITDRLPGSNNLDYIDYSNDNSAWRPFQLAYILTCLSSIENPDSKHRDLVDLLWIPTG
                     GGKTEAYLGLSAFTIFLRRIRNKENGNGTAIIMRYTLRLLAAQQFIRASILICACEII
                     RKENKSELGKKEISIGLWIGSDQTPNFNDEAEEMLNNLTGNNIKFRSSINSEYELDNL
                     KSKYNKFQLLKCPWCGTKLVKEWDKTSKKAVGDWGYRIKSRPKKFYLCCPEEDCSFED
                     MLPIQVVDEEIYNNPPTLLFGTVDKFAMITWTTEVASLFALDDDNSNLSPELIIQDEL
                     HLISGPLGTMVGLYETALDAMCSYKGIKPKIIASTATIRKAQNQVKNLYNRNVAQFPP
                     PGLTSSNSFFVKEMPLSKKRGRLYVGVIPSGKTQVTVEVRLMSALLQRLKMMENVELN
                     LLDKYWTLVSYFNSIRELGKCSTLLNDDIKDNMERLCKRVSAHPYQSLRNLNEQRELT
                     SRLTSTKINTTLDELSNTLTSNNLTVDTLLASNMISVGVDVDRLNLMVVVGQPKLTSE
                     YIQASSRVGRSNEGLIFTLYNPTKTRDRSHYEQFFSYHQSFYKFVEPTSITPFSEPAR
                     ERGMHAVLISMIRHILGLQANDLASRFSKNMDGVEDLKNFIIDRSIDIFRENNQNADD
                     NIVNEFKSEISSELDIIMDLWHDKAQISDKDNLLHYYKRPSKFDNKIYNNLLIPYDKD
                     VITNEKQTLNSMRNVDKQCTVEIIELEEN"
     gene            4465..6378
                     /locus_tag="JA154_14620"
     CDS             4465..6378
                     /locus_tag="JA154_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997078.1"
                     /translation="MSIKLDDKTHNLRINQATSVFGPGAMLDFIDQTLMTAHPNYWTN
                     TIKIQDDRLQATLRVNELRTPPTIDDRSSVPFVRFPRWYFCPKCRKFMPIEDWEKLYK
                     NAHKDKPMKIPICLTCSTKKSTKQLVPTGIITICEHGHIDDFPWVKWVHFRNKSGRKE
                     ICENPQILMKTGKSNLGLEGIELECSCGAKANMKGAFMRLKSSKSNDYGVPSEYKEMF
                     KCSGNSPWYNKKVNKCTKYSFASQRGASNVYYPKIESSIIIPPYSDTLNSKVLSSKGY
                     DTFKKFLDRAIKKNKLERFMDEDFEDFASDIAKETGFDTDKVSIVLKDLLPFEDSSPS
                     TTTRNQYREEEYEALIGNIDAKSKKTNDFTIEEQNLSDYDIPNFSKIVLVKRLREVRA
                     LTGFSRVNPPDNNIFGEEDIDDSCNDTKVISLRSRDCKKSFYPASEVRGEGIFIEINN
                     DMVKSWISSNPKIQDNASILNSRYNKDINVNDKAYRKITPKFLLLHTLAHLIIRELSF
                     VCGYATSSLRERIYCNNSENEKVMSGILVYTASGDSEGTLGGLVRQGMHDSLPSILNS
                     ALNRALWCSNDPICIDSTGQGRNSLNLGACHSCTLLPETSCEEYNLLLDRSMLIGDMD
                     NRDIGFFKNLILI"
     gene            6628..6771
                     /locus_tag="JA154_14625"
     CDS             6628..6771
                     /locus_tag="JA154_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997079.1"
                     /translation="MDSNGGDRGEFISGYLIVLLVFISIGIWLFGHDVEAIFLVIFYC
                     LFL"
     gene            complement(7084..7833)
                     /locus_tag="JA154_14630"
     CDS             complement(7084..7833)
                     /locus_tag="JA154_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997080.1"
                     /translation="MQLSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKEN
                     HVFIDNKKIKIKFGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYI
                     VITIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSK
                     VKKLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNF
                     TKILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(7849..8163)
                     /locus_tag="JA154_14635"
     CDS             complement(7849..8163)
                     /locus_tag="JA154_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997081.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(8167..8367)
                     /locus_tag="JA154_14640"
     CDS             complement(8167..8367)
                     /locus_tag="JA154_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997082.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            8514..>9129
                     /locus_tag="JA154_14645"
     CDS             8514..>9129
                     /locus_tag="JA154_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7997083.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISNLME
                     DDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDIK
                     EIIKNDVLTKQSIDELLDIGLSEESIETIYDYFSDSTDNGSNIRL"
BASE COUNT         3291 a         1169 c         1361 g         3308 t
ORIGIN      
        1 aaaatatata acttacttga tataaaacct tttaaaccaa cttatgaaac caataaatta
       61 gaagcttatt ctaccgatag tcctatttct gatgatgatt ctatggatga atatatatct
      121 aattataaat ttgatataaa tttaaatgaa attatattat ctctatctat aaatcaaggg
      181 atttacaact ctaaaaaaga acatgttaca tcattcatga tttctgataa taatgagtat
      241 atattctgta caaaaaaggc aatagcctat tcaatagatt gggataaaaa aagtgttagg
      301 aaaaagaaac tttcagaggc taaagttaat gactggtttt tatttgttga tgattactta
      361 aatgaagagg gtgaattagt tattaatgtt atacaaaagc tattagaact taatttattg
      421 ccatctcatt atcttgatga ttataaatta actaattcat ggaaatcaga acttaaaaac
      481 tatatatctt atagtaaata taattataat gatgtatcaa acagtttagc tgttttaggt
      541 gaaaagatta atcctataac aatcaaaaac tgggtaacaa ataaaagatt aataggtcct
      601 atatatttaa aaactttcga aaagatattt aaagttatag attctaaatt agatcatacc
      661 gaggtatatg atgctactaa aagtatacgc tctttgcaca ctaaaataaa aaaatctata
      721 gatacatttg tatctgataa tttctttagc agtaaatcag atataattaa agatgaagtg
      781 tctgcagttg ttttaagtat gcttgggaat acaaatgatt atgttacacc tgtacaaata
      841 cgagaaatac acagttgtga taagtatctg tcaccccacc ttgttaacaa agttttagac
      901 ttatatagtg tttattaaat aaataggagg taaatatgaa ttgtagtgaa ctaagagata
      961 aaaccctaga tcttgtaaga aaagaacttc ttggacctgg ttcggaagat ctttgtgaaa
     1021 atataaaata tgaaataata acagatagtc caatagagcg ttattcaaca ggtattttat
     1081 atccacttaa taacaatatt gatgtaagta atacatctga atatgatggt gaagataatg
     1141 tatttacatc agatgataca caaaatgaac tagatagagc tataagtatg tctaatgaaa
     1201 ctttaccttc ttctatgggt atgacttttt tcctaaaaaa agatgcagaa gtaataaatt
     1261 tcagggtaaa ttatgctact tatagaaatg ctttatatac agactgtcat gttaaatata
     1321 ctggtgacga cttctattct agcaatacaa ttaatgaata tgtagaactt aggggatcat
     1381 tcctatatct taagcaaaaa ctaacatctg agaaggttta taatttacta aaaaagcatg
     1441 atttacttga taatgctgaa gctaaatttg ttttatacaa gttagctaac caatgccctg
     1501 atgatgagaa taagactggt ggttatgttc gtgttccaca tgaaaactta atagtatcaa
     1561 tagatatgga taaaaactat attaaaaaac ctatatctaa agatttagat atagttttat
     1621 ctaaaagaat ttattcaaat gactttgtta gtattacttg tatgctggtt aatagtcaat
     1681 ctgggcctaa gaaatataat aatactattt tccagcctga actaatggta ttagctgaag
     1741 ataacaatga tttagagttt atagatattt ttacagctgg atcagtaaat acttttaaaa
     1801 agttagattc tgaggaaata tctttagact tactttaccg aaataaaaaa acttttgcta
     1861 ctggtcatgg tgtagctata gatcaagata ttcaaaatga tgctttaata aaactttgta
     1921 ctgagtttat tcctcatttt gaagtaccta aattggactt taatgtagat gaattaagtg
     1981 atgtttctag agaattactg tctatgaaaa atttatcaga tatctctgat cttagtaaag
     2041 ataagaaaat atattttctt tataagtttg ttgatgctta tgatatttgg atagatgatc
     2101 tagaagaaga ttcaaaagat cttagtgatg tttttaaaga tgtagcatat gagcatataa
     2161 aaaactgcag agatactaag tctagaatgc tagaaggtat aaatttaatt aagtctaatg
     2221 atgaagtata cagagcattt tctctaatga atagagctat acttatgcaa agatgtcatt
     2281 ctatacatat tacggataga ctccctggat ctaataacct tgactatata gattactcaa
     2341 atgataattc tgcttggaga ccattccagc ttgcttatat acttacatgt ttatcttcga
     2401 tagaaaatcc tgactctaaa catagagatt tagttgacct tctttggatc cctactggtg
     2461 gaggtaaaac ggaggcctac ttaggtctat ctgcttttac tatcttctta agaagaataa
     2521 gaaacaaaga aaatggtaac ggtacagcta tcataatgag atatacacta aggctattgg
     2581 ctgcacaaca attcataaga gcaagtatat taatttgtgc ctgtgaaatt attagaaaag
     2641 aaaataagtc tgaacttggg aaaaaagaga tttctattgg tttgtggatt gggagtgatc
     2701 aaactccaaa ctttaatgat gaggcagaag aaatgcttaa taacttaaca ggtaataaca
     2761 ttaagtttag atctagtata aatagtgaat atgagttaga taatttaaaa tcaaaatata
     2821 ataaattcca attactaaaa tgtccttggt gtggaacaaa acttgttaaa gaatgggata
     2881 aaacttctaa aaaagctgtt ggtgattggg gctatagaat taaatctaga cctaagaaat
     2941 tctacttatg ctgtcctgaa gaagattgtt catttgaaga tatgttacct atacaagttg
     3001 tagatgagga gatttataat aaccctccaa cacttttatt tggtacagtt gataaatttg
     3061 caatgataac ttggactaca gaagtagcta gtctttttgc tttagatgat gataatagca
     3121 acttatctcc tgagttaata attcaagatg agcttcatct tatttctgga cctttaggta
     3181 ctatggtagg tttatatgaa actgcacttg atgctatgtg tagctataag ggtataaaac
     3241 ctaagattat agcttctact gctactataa gaaaagcaca aaatcaagtt aaaaatctat
     3301 ataatcgtaa tgtagctcag tttccacctc caggattaac atctagtaac tctttctttg
     3361 ttaaagagat gcctttaagt aaaaaacgtg gccgactata tgtaggtgtt attccatctg
     3421 gaaaaactca agttactgta gaggttagac ttatgtcagc attactccaa agacttaaaa
     3481 tgatggagaa tgtagagttg aatttattgg ataaatattg gacactagta tcttatttta
     3541 atagtatcag agaattaggt aagtgtagca cacttttaaa tgacgatata aaagacaaca
     3601 tggaaagatt atgtaaaaga gtctcagccc acccttatca atcattaagg aacctaaatg
     3661 aacaaagaga gcttactagt agacttactt ctactaagat aaatacaact ttagatgaac
     3721 tatctaatac tcttacttct aataatttaa ctgtcgatac tctattagct agtaatatga
     3781 tttcagttgg tgttgatgtt gatagattaa atttaatggt agtagttggt cagcctaagt
     3841 taacatctga atacatacaa gctagcagta gagttggtag aagtaatgaa ggacttatat
     3901 ttacattata taatcctaca aaaacaagag atagatctca ttatgaacag ttctttagtt
     3961 atcatcaatc attctataaa tttgttgaac ctactagtat tacacctttc tctgaaccag
     4021 ctagagaaag aggcatgcat gcagtattaa taagtatgat tagacatatt ttaggacttc
     4081 aagctaatga tcttgcaagt cgattttcaa aaaatatgga tggtgtagaa gatttaaaaa
     4141 acttcataat agatagatct atcgatatat ttagagaaaa taatcaaaat gctgatgata
     4201 atatagtaaa tgaatttaaa tctgagattt catctgaatt agatattata atggatttat
     4261 ggcatgataa agctcaaata tctgataaag ataatttatt gcattattat aagcgcccta
     4321 gtaaatttga taataaaata tataataatc ttttaattcc ttatgataag gatgttatta
     4381 caaatgaaaa acaaacatta aattctatga gaaatgttga taaacaatgt actgttgaaa
     4441 ttatagaatt ggaggaaaat taatatgtct ataaaattag atgataaaac tcataattta
     4501 agaataaatc aagctacaag tgtttttgga ccaggagcta tgctagattt tattgatcaa
     4561 acattaatga ctgctcatcc aaattattgg actaatacaa ttaaaataca agatgatagg
     4621 cttcaggcta cattgagggt taatgaacta agaacacctc caactataga tgatagatct
     4681 tctgttcctt ttgttaggtt tcctagatgg tacttttgtc caaaatgtag aaaatttatg
     4741 cctatagaag attgggagaa actttataaa aatgctcata aagataaacc tatgaaaata
     4801 cctatttgcc ttacttgtag cacaaaaaag tctactaaac aattagtacc tactggtata
     4861 attactattt gtgaacatgg tcatattgat gattttccat gggtaaaatg ggttcatttt
     4921 agaaataaat ctggaagaaa agaaatttgt gaaaatcctc aaatattaat gaagactgga
     4981 aaaagtaacc taggattaga aggtatagaa cttgaatgta gctgtggagc taaagctaat
     5041 atgaagggcg catttatgcg tttaaaatct tctaaatcta atgattatgg tgtacctagt
     5101 gaatataagg aaatgtttaa atgctctggt aattcgcctt ggtataataa aaaagtaaat
     5161 aaatgcacta aatattcatt tgcttctcaa agaggtgcct ctaatgtata ttatcctaag
     5221 atagaaagtt caattattat accaccatac tctgatactc ttaattctaa agtgctatct
     5281 agtaaaggtt atgatacatt taagaaattc ttagatcgtg ctattaaaaa gaataagtta
     5341 gaacgcttta tggatgagga ttttgaggac tttgccagtg atattgctaa agaaacaggt
     5401 tttgatactg ataaagtatc catagttttg aaagatttat tgccttttga agattcttca
     5461 ccatcaacaa caactagaaa tcaatataga gaagaagaat atgaggcttt aattggaaat
     5521 atagatgcta aatctaaaaa aactaatgac tttacaatag aagaacaaaa tctatctgat
     5581 tatgatattc caaacttcag taaaatagtg ttagtaaaaa gacttagaga agtaagggca
     5641 ttaactgggt ttagtagagt caatccacct gataataata tatttggtga agaagatata
     5701 gatgatagct gtaatgatac taaggttata agtttaagat ctagagattg taaaaaatct
     5761 ttttatcctg catctgaggt tagaggtgaa ggtattttca tagagataaa taatgatatg
     5821 gtaaaaagct ggatttcatc taatcctaaa atacaggata atgcatctat tttaaattct
     5881 agatataata aagatattaa tgttaatgac aaagcttata gaaaaattac acctaaattc
     5941 ttattactac atactttagc tcatttaatt ataagagaac ttagttttgt ttgtggatat
     6001 gctacctctt cacttaggga acgtatatat tgtaataatt ccgaaaatga aaaagttatg
     6061 tctggaattt tagtttacac tgcaagtggt gactctgaag gtactttagg aggattagtt
     6121 aggcaaggta tgcatgattc attaccatct atattaaata gtgctcttaa tcgtgctctt
     6181 tggtgttcta atgacccaat atgtattgat agtactggtc aaggtagaaa ttctttaaat
     6241 ctaggagcat gccattcatg tactttattg cctgaaacta gctgtgagga gtataattta
     6301 ttacttgata gaagcatgtt aattggtgat atggataata gagatattgg gttctttaaa
     6361 aatctaattt taatttagta ataactaatt ttttatttat ataactaaag gttaaaagtt
     6421 aattttatga cttctatcct ttagtttttt gtatttaaat tattattttt tgcaaatcat
     6481 tcttactaga aattacttat tataactaat tcccaataat tatataattt tgtgctcata
     6541 ctgaaatttt taaataaact attaatttat attatatatg atttttcaaa ataaaaaaca
     6601 tattaactat taaatcaata tgttttcgtg gattccaatg gtggagaccg aggagaattt
     6661 ataagtggtt atttgattgt tttgttggtt tttataagta taggtatatg gctatttggt
     6721 catgatgttg aagcaatttt tctggttatt ttttattgtc tatttttgta gttggggtat
     6781 tattggggta taattttcta ctttccaaaa ttattgatac ttcaataata taaattaaat
     6841 tcttagttaa gttttttaat gaataggtaa atattatttg tactcataac aatatagttt
     6901 acgcataatt ttatatatat tttctggtat atctgcaact aacttagtgt aatttaaacg
     6961 atattagaga tacttaaaaa acaaatatat gaaagattta taactaactt agtgtaattt
     7021 aattgattgc tctgcttctg tatttttaat taagttgttt gaattattta taatataacc
     7081 tccttaccac tttaattgtg agtcatctgg attatatatt cctgtctctt tatttatgac
     7141 aatttccttg ctattataat ttccctctag tatcttagta aaattacttg aattcattat
     7201 ccaatcaaaa tttgctttcc atcctgtatt attatttcca ttcagaaaac ttgttttact
     7261 aactatattt ataacctcta atacagtttc tttatcatat tcttttattc ttgaatttat
     7321 tctcctcttt cttgtatctg ttagtttctt gacctttgaa agttcaatac aagttgagtt
     7381 aaatttgctt atgatttcac tatatataat attattttca ttctttatat tcttgattgt
     7441 tgttgactga ttgttaatag tctgttgatt aggttgttga ttttctatat tctcttgata
     7501 aagctcccag ttttcaatag ttattactat atacttgttt gttgtttcgt atgttagaaa
     7561 accagcattt ttaaaaacca acaaacaact tctaaccatt tgataactaa ctcctttacc
     7621 acatctttca gctattttgc tatatgaaga aataaattgt ccaaacttta tttttatctt
     7681 tttattatct ataaaaacat gattttcttt atgactagcc ataagtaata gagttattaa
     7741 tattaccttt ctttctggtt taatatcttt ccaaattgat ttatccaaca actccctata
     7801 aagctttatc caccctttat tgctcaattg caaacaccta ctttaagact ataacttgat
     7861 accatttgca ttttcaataa accaatcatc taatttattt tttattatgt atactcgatt
     7921 atttatccat atacaaggga atcctttgtt tttagaatga cataactgcc taaattttga
     7981 ttctcctata ggataaagct tagatgcttc tttacatgtt ataagctcag ctttatttct
     8041 tttcgcattt tcaataattt tatatccttg ctttttttct tgtgcttctt cttcattctt
     8101 tacaataatg tttaaagtgg cttgtaatgt ctttaaaacc tctaaattat tattctcttt
     8161 catattttaa tcctccttac taaaaaataa ttcttctact gttgtatcta aagctctaga
     8221 tagtttaatc atcaaatcat tgcttggatt attagcctta cctgtttcca agtttgataa
     8281 ataatatctt gatataccaa ctctatctgc cagttcttgt tgctttagac cttttttaat
     8341 cctaaatatt tttaaattga ttcccatact ttttcctcct tttttctagt ttacattcgt
     8401 aaactttaca ttttaattat aatgtcgata tatgtgtact gttaataaga atatattatt
     8461 tttctgttct catttgtgta cttttgggat atactttata cgaggtgata atattgcaaa
     8521 ttggaaaaaa cttaaaaaag ataagaaaac aaaaagagct aacgcagatt caacttgctg
     8581 aaattagtgg catctcaaga aatgctctaa taaattatga aaatgataaa agaataccta
     8641 gtatagatac cttatcaaaa ctcgctaaag ctttgaaaat tgaaaagact atcttttatt
     8701 ttgatattga aaatgaagta atgactaatg aagatgtttt atacctttta cagtctttat
     8761 tcccagaaga atactattct tttgatgaat ttttaaatgc tatttcaaat cttatggaag
     8821 atgatattag aattacctca agtttattgc taggtgcctg gtctgataaa gaccaatatt
     8881 ataaacttgg tgaaaaatta gaactaaatg aaaatcaagt ttataattgg atactttcag
     8941 attgtatatc taatactgaa ttagattttt cattacatcc tgatgacata aaagaaatta
     9001 ttaaaaatga tgttcttaca aaacaatcaa ttgatgaatt attagatatt ggtctatcag
     9061 aagaaagtat agaaacaatc tatgattact ttagtgactc aacagataat ggaagtaata
     9121 ttcgactaa
//
LOCUS       DACULQ010000151         9048 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.152, whole genome shotgun sequence.
ACCESSION   DACULQ010000151 DACULQ010000000
VERSION     DACULQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.152"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            192..1397
                     /locus_tag="JA154_14650"
     CDS             192..1397
                     /locus_tag="JA154_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAT7997084.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1468..1689)
                     /locus_tag="JA154_14655"
     CDS             complement(1468..1689)
                     /locus_tag="JA154_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997085.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1830..2429)
                     /locus_tag="JA154_14660"
     CDS             complement(1830..2429)
                     /locus_tag="JA154_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAT7997086.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2442..2792)
                     /locus_tag="JA154_14665"
     CDS             complement(2442..2792)
                     /locus_tag="JA154_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAT7997087.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2851..4488)
                     /locus_tag="JA154_14670"
     CDS             complement(2851..4488)
                     /locus_tag="JA154_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAT7997088.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4565..4829)
                     /gene="ffs"
                     /locus_tag="JA154_14675"
     ncRNA           complement(4565..4829)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JA154_14675"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4867..4958)
                     /locus_tag="JA154_14680"
     tRNA            complement(4867..4958)
                     /locus_tag="JA154_14680"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4922..4924),aa:Ser,seq:gga)
     gene            complement(4959..5414)
                     /locus_tag="JA154_14685"
     CDS             complement(4959..5414)
                     /locus_tag="JA154_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HAT7997089.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5735..7006)
                     /locus_tag="JA154_14690"
     CDS             complement(5735..7006)
                     /locus_tag="JA154_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAT7997090.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7132..7337)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7465..8346)
                     /locus_tag="JA154_14695"
     CDS             complement(7465..8346)
                     /locus_tag="JA154_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAT7997091.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8358..8885)
                     /locus_tag="JA154_14700"
     CDS             complement(8358..8885)
                     /locus_tag="JA154_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAT7997092.1"
                     /translation="MERRCNMNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIV
                     QDIALIRARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIK
                     DVIVNHPTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEET
                     IEAIVKELKDNGILS"
BASE COUNT         2895 a         1600 c          976 g         3577 t
ORIGIN      
        1 tttatttaca agaatatttt taatataaca tatttgataa aattaattta catttctttc
       61 acttgtaatt tttgcaatct tgtattagat taatattaaa aataatttat caataacctt
      121 attcacaaag acagttttta tatatcaaaa tataatataa atactatgct acattttagg
      181 aggattttat tatgagcaaa aaagtattaa taatgtctgc ttccactggt ggtgggcata
      241 atagggctgc tctagctatt aaagaagaac ttacttccaa aacacttgat ggagagccaa
      301 ttgaatgtga aataatagat agtttgaaat tagttaataa tacaatggat aaaataattt
      361 caagaggata cgaaaaatct gctatatata ctcccaaagc ctatggaagt gtatatagat
      421 tatctgaaac aaatcttttg tcaaaaaatg aatttaaaga taatctacta ataacattca
      481 tggctaaaaa atttaaaaag cttattagaa gcgaaaaacc agacttgatt ataggaacgc
      541 atccttttcc aatgattgct ctaagtacat tgaaaaaaaa ctttaattta cataataatg
      601 aatcaaatgc ttacacagag catttttata aacattatac aaataccatt aatgttcctc
      661 cgcttatttc tgtattaacc gattatacta ctcattctac atggatacaa aatgaaattg
      721 attactatat tgttggtcat gaatatgtaa aagaattatt ggtgtttgat ggagttgaac
      781 cgagtaaaat tagaacattt ggaatccctg ttgaaaaatc ttttctctct cacagagata
      841 aagatattgt tctttctgag ttgaatttat ctcctgataa attaactgtt ttacttatgg
      901 ggggtagctt tggggctgga aatataaaag agacacttga tgaattactg gatactgata
      961 gagactttca aatacttgta attactggta agaatgaaag cctaaaagaa aaaatcgaaa
     1021 aaaaacttat gtctcgttat catgataaaa atgtatgtgt ccttggatac actgataaga
     1081 tgaatgatat tttagcttct atagatgtat tagtaagcaa acctggtgga cttactacaa
     1141 ctgaggctct tttaaaagat gtacctatga tagttcctta ttatatacca ggtcaggaag
     1201 aagagaatct tgatttctta tctaattgtg gtgcagcact tagaactaca aaaaaatata
     1261 acttgtcaat attactaaaa gttttaatag atgaccctag cagattagaa atgctaaaaa
     1321 gaaatattaa atcaataaga aagtcaaatt cagcccaaaa tatagctaat ttaattttaa
     1381 atataatttc tggttgataa aataaataaa atccctatag tatatatatt atctattagg
     1441 gattttttat tttctaatta tttaatatta aattggctca taaaagtctt cttcataatc
     1501 atactgataa tttttataat tatttctaaa ttcagcattt ttatttcgta catttttttt
     1561 agtttttttc ttattagaaa aacttatatc ctcaaaatca tcatatttag gtttatcttt
     1621 tgatttcatt tttttaactc ttttttcttt cattgcatta tgttcttcta agttcataat
     1681 tggtttcatg ttgcacccct atcaaaattt ttaaatgatt tatacaacaa attatacaat
     1741 acagaagaaa tttattcaat aaatctaaat caaaattaat ttgaatttaa ctcaaaaaac
     1801 tacaaaaatc gaggtattaa cctcgatttt tatatttctc ttctgccttc tagtgctttt
     1861 gatattgtga cctcatctgc atactctaaa tcgcctccaa caggaagacc atgagctatc
     1921 cgagtgactt tgattcccat tggttttact aacctagcaa tatacatggc tgtagcttca
     1981 ccttcaatag taggatttgt tgccattatt atttctttga cttcctgatt gccaagtctt
     2041 tggataagtt cctttatttt taacatatct ggtccaattc catccattgg agaaattact
     2101 ccatttaata catgatactg tcctttaaac tctctcgttc tctccatagc tgctacatct
     2161 cttggatctt ccacaacaca tataacactt gaatctctac ttttatttga acacatgcta
     2221 catgggtctg tatctgttat attacaacaa attgagcaat atcttatttc tctttttgca
     2281 tctataatcg ctttagataa agcttccaca tcattagtat tcatattaat aatatgaaaa
     2341 gccaatcttt gagcagtctt tcttcctact ccaggaagct ttgaaaattc ctctataagt
     2401 ctcgttatgg gacctgtata aacttgcata aataccacct actagaataa cccaggtata
     2461 ttcatacctc cagttacctt actcatttga cttgcttgta tatcatcaat atttctcaat
     2521 gcttgattta ctgcacttaa aactaaatct tgtagcattt ctatatcatc tggatcaact
     2581 acttctggct tgatagttat atctataact tcttttttac cattaacttt tacagtaact
     2641 gctcctccac caactgaagc ttctacttct ttactttcta actcttgttg agctttttgc
     2701 atattttctt gcattttttg agcctgcttt aacaagttgt tcatatttcc accaccaggc
     2761 atcattccgc ctccaaaacc ttttttagcc ataaacacta ttcctcctaa acttattttc
     2821 attttattat tatatcataa tagtatttta ttatttactc tcattttcat caatactatc
     2881 ttttatttct aatatctctt cactcactat gttctttaat atttcctcac caatgtcctt
     2941 tttctcttta acttcaagat ttatatcttt aacttctgat gtcagtacta tttttacatt
     3001 aaaacttcta tttaaaacat ctcttattgt tttttctaaa taatttattg tatcttgaga
     3061 gctcagtcta gtttttgcaa aatcaaattt atcatcaaat ataacataca acatattaga
     3121 atatacatta aaacttttta cttcacttaa gagtgcgtat attggcattt ttttatcttt
     3181 tttaatttgt ttaagtatat tcttccaaga tgactctata agtataacat cctcattttt
     3241 tacatcttca taaactacat tttcttgttt tacatctatt tctctatcga cctcatactc
     3301 tttttcttta ttattcccaa ttttttcaga cttaaagttc cccagttcaa tcattttttc
     3361 taagttctca actcttttta taagagcttc tttactttca tcaaacattg gttgagcaat
     3421 tttcattatg gttatttcca ctaaaaccct tggatttgaa gatgacttta tatcatcctg
     3481 agttattgat aatatattta aaatccttat taaatcattt atatctatat ttttagactg
     3541 aattttaagt tgttctatag tttcttctgg taatgaaatt atttcatcca attctgatga
     3601 aaccttacat accattaaat ttctaaaatg atcaattaag tcatttataa gatttcttat
     3661 atctttgccc cataagacaa attcatttaa tatctcaaga gactttttag tgtcctgctc
     3721 tacaatacat tgagacaatt caaataattg ttctatattt acgcttccca ttaattcaac
     3781 aacatcttta taatctatct tactttctcc aaaagacata cattggtcta atatactaag
     3841 tgcatctcta agagctcctt gagaatttct agctattaaa ttaagagctt tatcatctat
     3901 atcaattcct tcatcttcac aaatcttttt cattctaagg gtcatatctt taactgtaac
     3961 tcttttaaaa tcgtatcttt ggcatcttga taaaatggtt gcaggtattt tgtgaggctc
     4021 tgtagttgct aatataaaaa ttacatatga tggaggttcc tctaaggtct ttaataatgc
     4081 attaaaagct ccttgagaca acatatgaac ttcatctatt atatatactt tatatttagc
     4141 attggctgga gaatatttca cactttctct aagctctctt atatcatcta cactattatt
     4201 tgaagctgca tctatttcta caacatccat tgtattatct tttaatacag attcgcatat
     4261 ctcacactca ttacacggtt cttcatttat tttattcaaa cagtttactg ctcttgaaaa
     4321 aatctttgca gtagatgttt taccagtacc tcttgttcca caaaacaaat aagcatgtcc
     4381 tatattatca ctatatattt gattctttaa agtcttaata atatgatctt gaccaataac
     4441 atcttcaaat ttttgtggtc tatatgctct atataatgct ttatgcatct ttttacctct
     4501 ctttagcaaa attaataaaa aaatccctca ttatctaagg gattagtctt ttatatatta
     4561 aatgctgtac accatctatc gacttaattc cataggcgtt accaaaacag ttagctcaac
     4621 ttaggcaccc ccacggcaca caaaagtgat cacttaccgc tgcttccttc cggatctgac
     4681 ggggttcatg agctttcatt gcgcaggacc taagcatcaa caccacttat ttagggcggc
     4741 cctacagaaa ataagccttt agatggaatt caatcctgct atagcggatt gcaggttaca
     4801 gggcaccgct acctccccat ctagtacagc aaaattacaa ccatatttaa ttactttcat
     4861 tgtttctggc ggagagagtg ggatttgaac ccacgataca gttttaccca tatactcgct
     4921 ttccaggcga gcgccttcga ccactcgacc atctctcctt aatctctttt tctcaatgcc
     4981 ttaaaaaatt cttgcaatat gatcgagcat tcttcttgca aaacatcaaa agctactttt
     5041 acattattat tattaaaata atctaattta aactcatgct gtttttctat atatgaattt
     5101 tttacatgtc ttgtccctat tataagcttt tttattctag aattaactat tgcgccacta
     5161 cacattatgc aaggttccat tgtaacatat aaatcacaat cagtcaacct ccaaccacct
     5221 aatttttctg atgcttgttt tatcgctaaa atttctgcat gtgccgtaga gtcttttaac
     5281 gtttctgtaa ggttatgagc tctagctatt atttgattat ctttaactat aatagcacct
     5341 attggtgttt cttttttatt atatgcttta tatgcttctt ttaatgcttc tttcatataa
     5401 aatgaacttt ccataattcc tcccaaaact tataacaaac ttatcttatc ataattcatt
     5461 ttataattca acttaatttt attttgttta tttaattatt actatatata gctatatttt
     5521 ttttaaatta gttctaaaat aatttttaga tattcataca agagtgtttt ataaaaatat
     5581 ttagtgtttt atgatgaaaa ttctgctgtg taaattaaca taaaaaaact atgaaattaa
     5641 tatttataat aatttcatag tttttttatt tattgtttac tcataatata ttctcttcat
     5701 caaagcatac tctacatata caattaatat aaatttattt tataacatca acacccatat
     5761 aaggtcttaa ttcttctgga actactacag aaccatctgc ctgctgataa ttttctaata
     5821 tagcagccaa acatcttcct atcgctaatc cagaaccatt taatgtatga acatattctg
     5881 cttttgattt tttatccctt ttaaatctta ttcctgctct tcttgcttgg aaatcttcaa
     5941 aatttgaaca agaagatatt tctacatatc tattgtagct tggcatccaa acttctaaat
     6001 catatttaaa tgcagctgta aatcccaaat ctcctgtaca tatttttaca actctatatg
     6061 gaagacctaa catttgtaac atagtttcag catcatgagt taatgattct aattcattat
     6121 aagaatcttc tggtttaaca aatttaacta attcaacttt attaaattga tgttgtctaa
     6181 ctaaacctct tgtatctctt cctgctgaac ctgcttcaga tctaaaacaa ggagtatatg
     6241 cacagtattt aattggaagc tctgcaacat ctaatatttc attagcatgt atattagtaa
     6301 caggaacttc agctgttggt attaagaaat actctaatcc ttctatttta aacatatctt
     6361 cttcaaattt aggaagttga ccagtcccta taaaactatt tctattggcc ataaatggag
     6421 gtaagacttc cgtatagcca tgcttagctg tatgagtatt taaaaagaaa ttcattaaag
     6481 atctttctaa tctagctcct aatcccctat ataaagtaaa ccttgaccca gttattttcc
     6541 ctgctctttc aaaatctaaa atacctagac ctgtacctat atcccaatgc gctttatgct
     6601 caaaatcaaa tttagtaggt tctccccaag ttcttatttg aacattgtct tcatctgttt
     6661 caccttgtgg tacctctgga tgaggaacat ttggtattct cattaatgta tattccattt
     6721 ttgcttctac ttcttttact ttctcatcaa taccttttat cttatctgat aactctttca
     6781 atcttgcctt ttcttctgtt acatcttttc cttcttttat taattgaggt atttttttag
     6841 aatttacatt taactcattt ttcataactt caacttcttg aagtatttct cttcttttat
     6901 tatctaattc cactacagca tcaaggtcaa attctctttc acctcttctt tccatagctt
     6961 ttttgatatc atctagattt tctcttattc ttttaatatc taacatatta attcctccta
     7021 ataattttga taatataagt tataattcca acttaattta caaacattat acttgtaaac
     7081 tcttagttta aacaaatttt aaaaataaaa aaagacctcc gtttacccta taaaagggac
     7141 gaaaatcttt tatccgcgtt gccaccctaa ttgactatac taaaatgtat aatcctctca
     7201 gacctataac gtaggtaaac gtctagcttt aactagaagc tctagagtag attcaaaaac
     7261 cattgtaatg agttttcacc atccactcat tctctgaata caatctgttt tttactattt
     7321 tccttcacag ctttttaact aattgtttta tttgataatc attatattat atatattaac
     7381 atctttcaat actattattc aaattttttt agaattttct ttaatttttt atattaaatt
     7441 cattttttct atttaattaa gaccttatct tttatccatt gtatttagat tacctgtatt
     7501 attaaataat aatgaagaag gaacaaatgg aacttttata tcattctcat caaatttatt
     7561 ttttaccttt tccattacat caaagtatac atcccagtaa tcatcagttt ttacccaagg
     7621 tctagcaact atttgcatgg tgttatttcc tatagagttt agacctatta taggctttgg
     7681 atcatttaat attctttttt cgtttgtaaa aatttcattt aatatagact taactacatc
     7741 tatatcacta tcatatgaaa tattatatga aaaatctatt cttcttttgt tttgatgtgt
     7801 gtaattaatt atattattac ttgtcagttg aaaattaggt ataattatag ttttattatc
     7861 tggcgtctta agaacagtgc taaatatttg tatgtcagaa actgtacctt ctacacctga
     7921 accttgaata taatcaccta tattaaatgg cttaaaaaat aaaataatca taccagagcc
     7981 taaatttgat aatatttctt taaaagctaa acctatacta aatccagcag ctcctaacac
     8041 agctacaact gatgtagttt gtatccctat aatacctaag cttatcaatg atataataga
     8101 taacatagat atatatattc catatacaat aaaattattt acacctttac tcattccata
     8161 tttgattaga aactttgaaa ccatccttct tactatttta gatatataaa tacctatcac
     8221 aaatacaatt attgcatata taatatttgg tatattatca ctaaaattat cgagcatatt
     8281 ttctaaagtt ctcttagtta tattgctatt tgttgaggca gtcttagcca atgataaaaa
     8341 attcattcaa ctcctcctta tgataatatt ccgttgtcct ttaactcttt tactatagct
     8401 tctatagttt cttcttttat tgcctcaatt gtatgaatat ggctacattc tgtcagaacc
     8461 gataattgtt taaattcatt tgtctcaact ttcttcataa agttgtctat atccatttta
     8521 gaataaatat taagttctgc agttatctga ccatatgttg gatgatttac aataacatct
     8581 tttataatac cacctaaatc tacaattatc tttaattcat cataaatttc tttagaatcc
     8641 ttatgatttt tacactttat ttgttttgta aaatacggtt gattatatat tatatatcct
     8701 tgaggtgtag ctattatttc aaatcctcta gctcttataa gggctatatc ttgaactatt
     8761 acttgcctac ttacatttaa tagttcagct agtgttccac cttttacagg atgcttagat
     8821 tcttttaaaa tatctattaa tttacttctt ctttcattag aattcatatt acaccttctt
     8881 tccaaataaa aactttacct atactatata attttatatt ttatcttatt ataaatcaat
     8941 attgacttta tattctattt aatcttaact tttataataa ataaaaaaga ctacgtggct
     9001 acgtcctact ctcccaggca gtttcccacc aagtaccatc agcgctag
//
LOCUS       DACULQ010000152         9015 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.153, whole genome shotgun sequence.
ACCESSION   DACULQ010000152 DACULQ010000000
VERSION     DACULQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.153"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            191..2200
                     /locus_tag="JA154_14705"
     CDS             191..2200
                     /locus_tag="JA154_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HAT7997093.1"
                     /translation="MKKLFTSETFLEYQFISDCQISPDGGYTAFIVKKADIKENGYTS
                     QVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRNEKVKEAVKNGKQCMSYYAL
                     SLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRPIVEGLPEEEQQKKINAYNK
                     RHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAITAPMFNVVGMKISDGKILY
                     IGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGFELYQNQVILNLTDALSYGN
                     GENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGAGQTTKVDGEYIYYTSTVDM
                     DCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCLGNGLPEVYTVENGTLCKKT
                     HLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYEVGKKYPAVLAIHGGPRLTY
                     GPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQFGDIDYIDFMEFTDTVLEK
                     YTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRSIANWSGMEGTTDIGYYFCK
                     GQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDYRCYMQEAFQMFSALKIHGC
                     PTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIKKDNIGQ"
     gene            2217..4172
                     /locus_tag="JA154_14710"
     CDS             2217..4172
                     /locus_tag="JA154_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HAT7997094.1"
                     /translation="MEKIKIENSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            4188..5378
                     /locus_tag="JA154_14715"
     CDS             4188..5378
                     /locus_tag="JA154_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAT7997095.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(5832..6014)
                     /locus_tag="JA154_14720"
     CDS             complement(5832..6014)
                     /locus_tag="JA154_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997096.1"
                     /translation="MITKDNTLKCEDFNIQYCALNNITPKYHPPFTIIEYPNYFKKEN
                     IRNGKPCIYKIKNTNF"
     gene            complement(6282..6410)
                     /locus_tag="JA154_14725"
     CDS             complement(6282..6410)
                     /locus_tag="JA154_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997097.1"
                     /translation="MNEILEIVNSHKHFLKLHKIGCIKYLLKVRIEYIKEKVILYE"
     gene            6527..6901
                     /locus_tag="JA154_14730"
     CDS             6527..6901
                     /locus_tag="JA154_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7997098.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            6903..7760
                     /locus_tag="JA154_14735"
     CDS             6903..7760
                     /locus_tag="JA154_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ABC transporter ATP-binding protein"
                     /protein_id="HAT7997099.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            7763..8401
                     /locus_tag="JA154_14740"
     CDS             7763..8401
                     /locus_tag="JA154_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAT7997100.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
BASE COUNT         3256 a         1070 c         1636 g         3053 t
ORIGIN      
        1 gaattaagca tggattaaat atactttaaa ttacaaatat tacaaggaag ccagaaggct
       61 tcctaaaaaa ataaccttta taaaattttt tattttaaat ttaaaatttt ttattttaaa
      121 tttaaaataa aaaatgaaag aggtggtaga gagtttgaaa aaaattacta aagaaaggat
      181 agaaaatact atgaaaaagc tatttacgag tgaaacattt ttggaatatc aatttataag
      241 tgattgccaa atatcaccag atggaggata tacagcattt attgtaaaaa aagctgatat
      301 caaagagaat ggttacacaa gccaagttta tatattaaac aataaaactg gcgaattaaa
      361 acaaataacg tctataaact ctgttggagc atatgcctgg gaagatgaaa atactatctt
      421 gttccctgct ttgcgaaatg aaaaagtaaa agaagctgtc aaaaacggta agcaatgcat
      481 gagttactat gctttatctt taaatggtgg agaagcagaa gaacttttta gactccctat
      541 caaaggtggt aaattgaatc ctataggaaa aggtttgtat gcagttattg attcttatga
      601 caatgaccga cctattgtag agggattgcc agaagaagaa caacagaaaa aaataaatgc
      661 ttataacaaa agacattatg aacatttcaa agaaattcct tatgctgtca atggggaggg
      721 atatattagc agaaaaagaa aacgcctata tgtatacgac agtaatataa atgagttgaa
      781 ggcaattaca gcacctatgt ttaatgttgt aggtatgaaa ataagtgatg gaaaaatctt
      841 atatataggg caagagttta aagatgtaaa aggtttgaaa aatggggtct atgtatttga
      901 tacaaacaca aacacaaatg tctgtatatt agaaaaagat aagtatatta taaaaggttt
      961 tgaattatat caaaatcaag tgatactaaa cctaactgat gctttatctt atggaaatgg
     1021 agaaaatgga gatttttata ctattgatat tgatacgaaa gaaatgaaac ttctttcagg
     1081 gcatcaacat cattgtattg gaaatacagt aacatctgat gttaaaatgg gtgctggtca
     1141 gacaacaaag gtagatggag aatatattta ttacacttct acagtagata tggactgcat
     1201 tattgaaaga atacatattc caacaggaaa acaggaaaaa gtaacacaga caggttcagt
     1261 tgattttata gatgtaaaag atggcaatat tgtatgtgtg ggttgtttag gaaatggatt
     1321 accagaagtt tatacagtag agaatggaac attgtgtaag aaaacacatt taaatgacca
     1381 tattttagag gaatataaaa tttctgtgcc agaatacata gagtcaaaag gaagttctaa
     1441 atgggaaata caaggatacg tatataaacc agtagattat gaggtgggga aaaaatatcc
     1501 agctgtattg gcgattcatg gaggaccaag attgacatat ggaccatatt ttatgcatga
     1561 gatacaggta tttacaagtg caggttactt tgtattcttc tgtaacccaa gggggtctga
     1621 aggtcgtggc aatgcttttg cagatattag gaaacagttt ggagatattg attatataga
     1681 ttttatggaa tttacagata ctgtacttga aaaatataca gacattgata aaacaaaatt
     1741 ggctgttgaa ggtggaagtt atggtggatt tatgacgaat tggataattg gtcatacaaa
     1801 ccgttttgct gttgcttgtg ctcagcgttc tattgcaaac tggagtggca tggagggtac
     1861 aacagacatt ggttattatt tctgcaaagg acaaacaggt gcttctcata tggaaaacca
     1921 cgagcttcaa tggaaacagt cacctcttgc ttatgctgat aaatgtgtta caccgacttt
     1981 atttttacat ggtgaaaaag actatagatg ctatatgcaa gaagcattcc agatgttttc
     2041 agcattgaaa attcatgggt gtcctacaaa actttgtcta tttgaaggag aaaaccatga
     2101 actgtctcgt tctggtagac caaaacaaaa gctacaaaga ttagttgaaa tgctagattg
     2161 gttttcagta tatataaaaa aagataacat aggacagtag aaaggagaat ataaaaatgg
     2221 aaaaaataaa gattgaaaat tcagatattt ttgaatatat attccctcat gatatttctt
     2281 gttcacctga tggaaagcat attgcttata ttatatcaaa tatcaacgag gaaaaagact
     2341 gctatgaaca tgacttatat gtgatggaca tcaaaacaga aaaacaaata catatgactc
     2401 aaacaaaaga tgtaacatca tttagttgga tttctaatac agaactcctt tttacatcta
     2461 aaagaaataa gccaaaatca ggtacaacag atttttacac tatttctata gaaggaggag
     2521 aagcaaaaaa agcattttct attccaaaag cttgtagcgt acctgtatca cttggaaata
     2581 aattgtggtt actgacaaca aaaaatccaa cggattctaa aaaatcagaa ccagataggg
     2641 cagtagaggg tgtagattat tggacattta cagataaacc ttttataaga gatggagaaa
     2701 atttttctca aagaagacgt gtaacattag aattatatca agaaggtgaa aatataacaa
     2761 aagccattac accaaaattt tgtgaagtag caggtgtaga cgtttcttct gataaaaaca
     2821 gaattttata tactggtcag atttatgaag attgtgcaac tcctttttct ggactttggg
     2881 agtatcatat agacagtggt gaaaccaaag aacttgtacc acaaggaaag taccaaataa
     2941 gtttggcaaa atatattggc aaagataaag taatgttgca agcttcaaca ttagaccgtt
     3001 ctatcacaca gaatcatgat atttttatac ttgatttgag tactaatgaa ataaatatga
     3061 ttgctagtcc agatggtatg tatgctacat tgctggatgt agatgcagta tatggtggtg
     3121 gacgtagtaa taaagttata ggagacaaat ttattggtgc aagaatttgc agaactatga
     3181 cagaatttaa tgagtttgat acaaagacag gtaatatacg tatcatcaca aaagtagatg
     3241 cttttacatc ttttgatatt tacgacaaca ccatgtacac agtaatgttg aaagattatg
     3301 agttagcaga aatatatagc attgatatga ctacaggtac aatgaaaaaa atgactgctt
     3361 tttctaaacc atatttagat actcataagg tttcattacc tgaaaaattg acgtttgtag
     3421 caaaaaataa agaagaagtt gatggttttg ttataccacc tatagatgct aaagaaggcg
     3481 aaaaatatcc tgctgtattg ttcattcatg gtgggcctaa atgggcatat ggctatatgt
     3541 ttacacactt gaagcagtgt gtaacatcaa agggaatgta tgtgatttat tgtaaccctc
     3601 atggtggtga cggttatggt gaaaaattct tagaaatggt agaaagatgg gggtatgttg
     3661 actatgagca tcttatggaa tttgtagata cttgtattga aaaatatcct gggattgatg
     3721 cagaccgctt aggggttgca gggggaagtt acggtggtta tatgacaaac tggataattg
     3781 gtcatacaga ccgtttcaaa gcagcagttt ctcaaagagg tattagtaac ttgattacag
     3841 cttctttaat tattgacttt ggggatagaa ttatgaagca gacttgtgga gataagacgc
     3901 cttggaatca tgaggaggtt ctttggaatc attcacctat taaatatgtg aaaaatgtta
     3961 agacaccaac attgttctta catagtgata gggattatag atgttttatg ggggatactt
     4021 tccaaatgtt tacagcatta aaacagcttg gtgtagatac agaaatgtat ttgtttcatg
     4081 gagatacaca tggattgtct agaaatggaa gaccatctaa tagaattgca agagcaaatg
     4141 ctattgtaga ttggtttgaa agatatttat aataaggggg ttattctatg agtgatatgt
     4201 tattatggtt tagtgtcgta ttagttttat ttgctattgg ggatttaatt gcaagcaaaa
     4261 caaaagcaaa agtatcagct gtatttgtta cactactttt atttttgatt ctatttgtca
     4321 caaaagcaat accagctgat attattgaaa aagcaggtat gaccgcagca gctagttgga
     4381 gtgttccaat gattatgttt agtatgggaa caatgttgaa tgttaaacaa tttatagacg
     4441 aatggagaac agtattgaca gcatggcttg gaattgtagc agttatagtc tgtgtctcat
     4501 tatgtattcc tttgtttgga aaatcaacag tattgacaag cataccagtt ataaatggag
     4561 ctttacctgc tactactatt atgacacaag cagcactaga aaaaggactg acacttgcag
     4621 cagctacagc aacagttgta tttgcaattc agaagtttat tggtacacca atagcttcaa
     4681 gagcagcgtt acaagaggca aaccgtcttt tagtagaata tcatgaagca aaatcaaaag
     4741 gcattgattt agcaaatgtt gatactgata agaaagaagc agagaataca ggcacaaaag
     4801 taaaacaagc tttctgtgaa aaatacgata aatattattc tacaaatgtt tgtatatttt
     4861 ttatagcatt attctcttat ctagggtatg aactttcaga gattattcat gtaaattatt
     4921 ctattgtatg tcttgttgtt ggtgttattg taacacgtat tggtattgtg ccaaaagaca
     4981 ttttggaaaa aggaaaaatc aagggattta tcaacatggt tgtgtttgca gctgttattc
     5041 cttctttagc aaaagtttct ttgacagatt taattagtct ttttgtacca attgtgggta
     5101 tgtttgcagc ttctatcatt ggtatattcc ttatgatgaa ggtgttacca ggttggaaaa
     5161 ttatcggttc taaacctctt gcttttggag tagggttttg tcagatgttg ggattcccaa
     5221 ctacatattt aatttcaaat gaagtgtgta atgctgttgg agaaacggaa gaagaaagag
     5281 catatttgat gtctaagatt atgcctaagc tggtagttgg tggtatggct tgtatgattt
     5341 ctattgtggt agctggtctt atggtaccaa tgttgtagat aggtttcata gttaaaaaag
     5401 tggtattaat ttataggaga agctaaaaag tagtattaat ttataaaata attttatatt
     5461 agttaatttg ttataagaat attttctttt tataattata attttgattt tataagatag
     5521 cagatttttt atttgctatg ctaatagtta taatttgttt ttatttgcta aaatgcaact
     5581 ttaatttgat ttatgtataa tatagggtat ttaataatat ttttgaagaa tttttataaa
     5641 aaattaaaaa gaggagaaaa aagggagtgc cacaaagtga actttttagt tctaaggcac
     5701 tcttttttgt acgaaatcga atatattttc aacgttttaa aagtgagaaa gagacttatc
     5761 tctagtttga gatagccaat tttaattatt aaaattattt tttgttttat ttatcaaaga
     5821 gtattcgtgt tttagaagtt agtatttttt attttatata tacaaggttt tccgtttcta
     5881 atgttttctt ttttaaaata attagggtat tcaattatag taaaaggtgg atggtatttt
     5941 ggagtgatat tatttaaagc acagtattga atgttgaaat cctcacattt tagagtatta
     6001 tcttttgtaa tcaaaaaaat cttctatact tatagcaagg ttacttctgc aaatattcca
     6061 tacataataa tatcccaatt taaatttttt taatatagag tttacctcta gtctacactc
     6121 ttgaataact ctttcctcta tccaaattct tataaattca tcccatttat tactatttat
     6181 ttattattat ctaaattttt tagtatgttt gtaattagaa atggatttat tcctttatgt
     6241 tttttataaa tagtttataa cttaatttat cattaaacaa atcattcata caggattact
     6301 ttttctttta tatattcaat tcttactttt aataaatatt taatacatcc tatcttatgt
     6361 agttttaaaa agtgtttatg gctattaaca atctctaata tttcattcat atttattttg
     6421 tcatagtaat attttacatc ttttatattg acaaaaaagt gctctaagtg tatagtgtgt
     6481 atatgataac atcacactga aagtgataaa aagggggtga taaaaaatga aagtgctcat
     6541 ttctaacaca tcagataagc cattatacca acaaattaaa gagcaaatta aagaagcaat
     6601 atttactgaa gaactgaaag aaggggatgc aatgccatcc ataagaaatt ttgctaatga
     6661 tttaaaagtt agtattttga ctattagacg agtgtatgaa gaacttgaaa atgaaggctt
     6721 tcttacacgc caagttggaa taggaacatt tatttcaact ggaaatttag aaatactaag
     6781 agactcaaag agacgtatag ttgaacaaaa aatggcagat atgattaaag atgcaaaagc
     6841 actaggaatt acacagatag agttacaaga aatgatggat attctatatg aggaggatta
     6901 atatggaaaa tattttagaa gtttataact taaataagac ttataccgat ttttcattat
     6961 ctaatataag ctttgcatta ccagaagatt gtataaccgg atttataggt gttaatggag
     7021 caggaaaaac aacaacaatc cgttcgatat tggatgtcgt aaagcgtgat agtggaaaaa
     7081 taaaaatctt tggtaaagat ttcgatggaa atgagaagga attaaaaaat cgaattggag
     7141 ttgtctttga tgattcatat ttctatgagg agttgtcaat ggcagaaatg aaaagtatta
     7201 ttgcaccttc atatgataat tggtgtgaag aagattatca acaaaatatg aagcgtttta
     7261 atcttaaccc aaaacaaaag attgatacac tttcaaaagg aatgaaaatg aaatttgctt
     7321 tagcattagc attatcgcat aaagctgagt ttttaattat ggatgagcct acaagtggtt
     7381 tagacccact aatccgtagt cagatacttg acatactaac aggatatatg aagaaatgtg
     7441 gtcgtggtgt tttcttttcc actcatataa catctgattt agacaaaata gcagatatga
     7501 ttgttatgat tgacaatgga cgtattatat tagagcgtga aaaagatact ctattggacg
     7561 aacatagaaa agtaaaaggg gatattcaat ttctaaatga tgaaaccaaa aaattatttt
     7621 taaatttaga aattacaggt tatggtttta tagggattac ctcaaaaatt aatgaagtgc
     7681 aaaaaaatat gccagatatt ttaatagagc gtcctacaat tgaagatatt atgcttgcat
     7741 atattgaaag gagagagtaa ttatgttgat tcatttagtt aaaaaagata ttctgattgt
     7801 aaaaaaatat gtattgttga tattatttat tgcaatagga atcccattat ttatattatg
     7861 gcgtttacct ccatttgcac aaatattggg atttgtacta tcaattatat tttcagaatt
     7921 tatgctgtgt cagtatctat tattaaagga gagtcaatat tcaaaagcat ctgtattact
     7981 ttgttcaact ccttatccta gacgtggctt agttcaatct aagtatgttt tatttgcaat
     8041 ggtatttatc tattgtacat tagtttactg gattgaaaat ttacttattc cacaaatagg
     8101 aacttttgac ttgacatata ttttgattgt tttattaata agttctatta tttatggagt
     8161 ttatatgcct attcaataca aattaggata tgaaacaaca aaattctttt tcgtgatgat
     8221 aatcatggct tcatcatttg tattaccatt aactatagct cacagcaatt ttgaaattcc
     8281 atttatatat cagtatccat ccatgatagt gaatattgta ttgttccttt taagtttaat
     8341 tatactgtta gcttcaatgt ttatttctat taaaattttt agtaataagg agcttgtata
     8401 aggtaagggg gacttttata tgccacattt accagattgg gctgttttac tttgtatctt
     8461 acttccaata gtatataaag cttgtcagat atttaaaagt aaaaaataga gtatatattt
     8521 atattgccat ttttattgag tgaagttaga tggtatgaaa aggttacttg aaaataaagt
     8581 tacttggtgt gggagagtaa catcataata ttatagatta tatgtttctt gtgaatcaat
     8641 ttgtattttt taatagggaa tatccagtaa ttttgttttc cattgattat ttttcaatta
     8701 tgagtatgat tgtgtgtatt gtataccatc ttttaaggtg tttttaaaaa attatatcgt
     8761 caaaatataa atctaaggaa tcctataaat aaagcttttt aattttagaa aactaataaa
     8821 aagtaggacg ttaataaaaa gaagctgtct caaaatagag ataagcttct ttttcattgc
     8881 tgaaatgacg aaaatataat tgatttaata taaaaaagag tgctttatga actatttatt
     8941 ttgaacagta gatacacttg cactcaattt agttactcct aagctttatg aactaaaagt
     9001 ttattttgaa gctta
//
LOCUS       DACULQ010000153         8981 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.154, whole genome shotgun sequence.
ACCESSION   DACULQ010000153 DACULQ010000000
VERSION     DACULQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.154"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..420
                     /locus_tag="JA154_14745"
     CDS             <1..420
                     /locus_tag="JA154_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAT7997101.1"
                     /translation="LLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTFESFKKG
                     IITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYEEFDVKN
                     LDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            535..1320
                     /locus_tag="JA154_14750"
     CDS             535..1320
                     /locus_tag="JA154_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997102.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDKKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            1606..2931
                     /locus_tag="JA154_14755"
     CDS             1606..2931
                     /locus_tag="JA154_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088671.1"
                     /note="catalyzes the formation of succinate semialdehyde
                     and glutamate from 4-aminobutanoate and 2-oxoglutarate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HAT7997103.1"
                     /translation="MPMSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYE
                     GNEYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIA
                     PGDNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNM
                     RRKMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGII
                     VPPVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGG
                     LPLGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYI
                     KAGFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIF
                     LGQSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(3006..3434)
                     /locus_tag="JA154_14760"
     CDS             complement(3006..3434)
                     /locus_tag="JA154_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997104.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(3525..4901)
                     /locus_tag="JA154_14765"
     CDS             complement(3525..4901)
                     /locus_tag="JA154_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HAT7997105.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIREEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(4902..5987)
                     /locus_tag="JA154_14770"
     CDS             complement(4902..5987)
                     /locus_tag="JA154_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HAT7997106.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(6023..7231)
                     /locus_tag="JA154_14775"
     CDS             complement(6023..7231)
                     /locus_tag="JA154_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HAT7997107.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLIVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDSGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(7246..7497)
                     /locus_tag="JA154_14780"
     CDS             complement(7246..7497)
                     /locus_tag="JA154_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HAT7997108.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(7878..8918)
                     /locus_tag="JA154_14785"
     CDS             complement(7878..8918)
                     /locus_tag="JA154_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAT7997109.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         3169 a         1282 c         1276 g         3254 t
ORIGIN      
        1 ttactaactt ttaaatttag acctgataga aaaatatcaa aaatattaat tgaagaaaat
       61 agggaaataa ttaaagatat aaataaagat acagtagata tagttgggac atttgagagc
      121 tttaaaaaag gaataataac tattaaagca ggttctaata acaaagtaaa gagaccagag
      181 aatttaattg attctgatgg aaatataaga atttatttaa atgaaaatac aaaattagta
      241 tcttatataa ttaccaaaga atcaaaaatg gatatagtta aatatgagga atttgatgtg
      301 aagaatttag atttattaag gagatatgat actattaatg tttggattga taaagaaaat
      361 cagccaaagg ataaacatgg tattatggct agtaaattat taattggatt gtatgaataa
      421 aatattaaac aagaataaaa gaataacaaa tataaaaaac gaaaaaacta tttctataat
      481 tatttttacg ttgtgcataa aataagaaca ttagtgaagg agtagagata aattatggag
      541 ataataaaat atatcttgta cttttttata tattcatttt taggatggac agtagaaagt
      601 ataggatgtt ctatagcaag caaaagaata atcaacagag gttttttaaa tggtccaata
      661 tgccctgttt atggttttgg agcggtaatt gtgatatcac tattgggaag atttaataat
      721 gtagtaatag tatttctatt aggaatgatt ttaactacga tactagaata ttttacagga
      781 tttattttag aaaccttatt tcatgctaaa tggtgggatt attctgacaa aaaattcaat
      841 ataaagggta gagtatgttt aaaaaatgca atatactttg gtgtaatgtc tgttttaata
      901 ataagattta tacatccatt tataaaatat tttgtaagta taattccata tagaatatta
      961 atttcaatgg ctataattac aacactttgg actatacttg atttgattgt tactattata
     1021 acacttaaaa aattagatat aaagttaaac ttattagacg atataattac agaccttaat
     1081 gatataaatg tgaaattgga taaatttgat agaggagaaa tacaagcttt atttaaaaca
     1141 ataaataacg acgaattaga agttagagaa aaaataaaca aaataaatag taagttggac
     1201 cgtatagagt ctaatattat tttacaaaaa agagttataa aagcttttcc acatataaaa
     1261 cataaaaaac aacaagagca actagaacat tttaaaaaaa ttattaatga gaaaaggtaa
     1321 ttatacaatt taatgaagac gaatagaata atggtattta taagatgatt ttcagaggac
     1381 tctataaaaa tagaatagag tcctttattt tttgaaaaat aatgtttatt tgataaaaat
     1441 aaaatatatt gtttctgaat aacaaatatt ttgaaaatta taaaaacata tattgacaat
     1501 ttgtgtcaaa aatactaaca ttaagtatat aaagaatttt atatgagaat atctttataa
     1561 atcgcaataa ttttaaaaca taaatttatt taaagagggg atgatttgcc aatgagcgag
     1621 ataactagtc aaatgataag tacagaagaa aaaaaatatg ttgctaaaac tcaaaagata
     1681 ccttattatc cagtagcatt taaatcaggt gatggtgcaa tgctatatga ttatgaaggt
     1741 aatgaatatg tagatttttt agcaagtgca ggctctgcaa atgtaggtca tggtaataag
     1801 gaaatctcac aagcagttaa agaacaaatg gatgatataa cacaatatac acttgcatat
     1861 ttccatagtg atcctcctgt aaaattagct gagaagcttg tggaaatagc ccctggagat
     1921 aatgataaaa aagtactata cagtgcaact ggttctgctt gtatagatgc tgctataaaa
     1981 ttagcaagag ggtatactgg aagaacaaag ataatatcta tgtgtgaatc ttatcatgga
     2041 agtacttatg gagctatatc tatatctgct ttaagtacaa atatgagaag aaaaatgggt
     2101 ccattgttac cagaagttta tcacttccat tatcctgata aaaatagaac agctaaagaa
     2161 tgtttagatg agatagaata tgcttttgca cactatctac cagcagaaga ggtagctgca
     2221 atatttatag agccaattgc aggagatgca ggaataatag tgcctccagt agagtgggta
     2281 caaggtttaa gtaaaatatg taaagaaaat ggaatattac ttgtaagtga tgaaatacaa
     2341 caaggtatgg gaagaacagg taagtggttt ggtatagaaa attttggtgt tgaagcagac
     2401 cttatagttc ttggaaaatc tgttggaggt ggattacctc taggagcagt tgttggaaga
     2461 acagaaataa tgcaaagtct tgatgcacca gcacatttat ttactttagc tggaaacaca
     2521 acagtatgtg tggctgcttt aaaatctata gagataattg aaaaagaaaa cttacttcaa
     2581 aagagtatag aaatgggaga ttatattaaa gcaggatttg aaaaactaaa agaaaaatac
     2641 gatataattg gagaaataag aggcatagga ttatctatag gagttgatat agttaaagga
     2701 aaaggttcta atgaaaaaca tccagatgct acagctaaga tatgttatag atgtatacaa
     2761 actggattaa taatgatatt cttaggacaa tctactttaa gagtgcaacc acctctagta
     2821 ataactaagg agcaagtaga taaagcaatg aatatcatag attctgcaat agatgattat
     2881 ttaaatggta gaattggtga tgaagtatat gaagtaactc aaggttggta aaataaatat
     2941 taaatgaaaa taggagttat ctcaatttta gaaaattatt tctgaaaaga gataactctt
     3001 ttaagttacc cacaaataag aaaattgttt gattcattaa agactatatt tcggatgtcg
     3061 tttttgtgag tatcagagac agtattacga gaataattac ctttattcgt actatctgtt
     3121 aatttagtta taattaagat agctaaaaga ataagaatta ctgtgtataa agcaataaca
     3181 tatggttttc ttttatattt gtatttgtta ttgaaaaaag aatggccata attattttta
     3241 tttactttag atgtatttgt attctttttc ttgttacctt tctttttatc atcctttttt
     3301 ctatttttag ggtaaggaat gactttgtgt gtattatcaa gtttgatatt tttatttttt
     3361 tgaatattgt tttccaggct agcttttttt tctccaaatt gaatagtttt attggtattg
     3421 ttgttatctt tcaatgttaa tctccctccc ttaaactttc cctttagata atattactac
     3481 taacctatgg aatatacaat aaataatata taagtaataa aattttatag gtataaatct
     3541 ctttctccac gctctaaggc agtcatattg ttagatacta tgtttcttat atcttctctt
     3601 tctatcttac ctatttcttc tgcgataagt tttttaccca taattttaag ttcttcatct
     3661 ccataatcga ttaaaaattc atttaatgta gtaagagcat ttggtgtaca tacattatgg
     3721 atttgtcgac ttttagctaa gctcatgaat ctatcgccag ttctaccttt acgataacaa
     3781 gctgtacaat aactaggtat atatccatca gtaatcagtt ctttaagcac ctcaacaggg
     3841 cttctatggt ctccaacttc gaattgttct gtattgtcac catcttcata tgctttatat
     3901 cctccaacac ctgttagaga acctgcacta atttgtgata caccatattt tagtaactca
     3961 tttctcattt cagcagtttc tctagtggac attatgattc cagtaaaagg aacagctaat
     4021 ctagttatag ctactatttt tttgaacata tcgtcagaaa ctaagtgagg aaaatcttct
     4081 aagctcatac cttcagcttt tttcaatcta gggaaagata tagtatggaa acctacacca
     4141 aatttttcct ctaagtgttc gttgtgcatc ataagtccta gaacttcaaa tttagggtca
     4201 gataaaccaa ataaaactcc tgcaccaaca tcatcaacac cagcttccat agctctatca
     4261 aaagctgtta aatggtaata atagtcattt tttatagatt gaccatgcat ttttataaat
     4321 gttggtttgt gataagtttc ttggaataat atataagttc ctattccttt ttcttttagt
     4381 aatttatact catctacact tgtagctgct atgttaacgt ttacccttct tatatttccg
     4441 ttttcattat atgttgaata aattgcatct aagcaatcta agatgtaatt tatatcacaa
     4501 tttttagggt cttcaccagc ttctagagca agtcttttat gacccatttt ttctaatatt
     4561 ttaacctctt cccttatctc ttccatggta agtttttttc ttttaaattt gttgcattta
     4621 ctaaaaccgc aataaacaca ttcatttaca cagtagtttg atacataaag aggggcaaat
     4681 aaaaccactc tattaccata tatttcattt tttatttgcc cagctatact aaataatcta
     4741 tctagttgtt ttttatcttc tacttcaaga agtgtagcta tatctttata actaagtttt
     4801 tcccttctat cagccttatc aagtaccttt tcaatatcat cagaagttga attttttgca
     4861 tcttctaata aagaattaat aagttcatgg tttatgaaca tttacaaacg cctccattca
     4921 atattatcac cgcgagaata gtcaacttca agtcctaatg acttgatgtt ttcttctaaa
     4981 ctttggtgat attcacaagc ttcttgacca gtaaaggctt tgttatcgta aagagaatat
     5041 tgttttctaa attcttgagg agaaaggttt ggcataatta cattgcaacc agctaaaaga
     5101 cctgcatttc taccactagg gtttatagat gccaaggctg ttgtagcggg taataaaact
     5161 tttggaagta gaagtcttgt aattgaaagc ataagtaatg tcttctctaa atctccatgt
     5221 ttataatcct taaaaattgt atcatggtgt ggaatgaaag gtcctattcc aaccatatga
     5281 ggatttaact cttttaggta taataaatca cgaacaaggt cttcgttgtt ctgaaatggt
     5341 gattcaacca taaatccagc acctacctga taaccaattt ctttaaggtt ttttaggcat
     5401 tcttttctag ttctaagctc aatgtttggc ggatgtaatt ttttataatg actgtctgta
     5461 gcagtttcat gtcttaaaag atatctatct gcaccacata aaaagtattt tttatacgaa
     5521 tcataactct tttctcctag tgatagagtt atggcacaat cctcatattt attttttata
     5581 cttgaaacaa tttcacatat tttatcatca gtaaaatatg catcttcacc accttgcaaa
     5641 acgaatgttc tgtatcctat tttataacct atatcacaac attcaagtat ttcatcaagt
     5701 gaaagcctgt atcttatgat atttttatta gaagattgta ttccacaata gtagcaatta
     5761 tttttacaat agtttgtaag ctctataagt cctctcaagt agactctatt tccataatgt
     5821 ttatttctca tgtcgctagc ttgtttataa agatagtttc ttaaattttg ttcgaagtta
     5881 tattcctcat tgtcgatatt ttctattaaa tatagtagtt catcaaaact taatgaatta
     5941 atgctgtaaa gtttatctat aaagtattta atcttatttt tagtcatatt caaatatata
     6001 attattaagt atagttaaat ttttaataat tgaaaagttc aagtgatctt tccaaaatac
     6061 cttttacata tgctattaag ataccatagt ttacaatagg tacattgaaa gattttgcct
     6121 tttctattct agataacatt ccagctctat tcatcataca agctccacag tgaactacaa
     6181 gggcatattt atttatatct tcagtgaagg atactccaga actaaattca aaagtaatgt
     6241 ttttgccagt tttctttcta atcatattag gaatttttac tgttccaata tcatcagttt
     6301 gtctatgatg ggtacatcct tcggccatca agattttatc accatctttt aaattttcta
     6361 aagcataagc gccattaatc aattctttaa ggtctccctt ttgtctagca aacaatatag
     6421 aaaaagatgt aagaggaata tcttttggtg tatctttatc tacttgtgga aatacttgag
     6481 agtcagtaat aacaagtttt ggttttttgc ctaaattaga tagagtttct tttaaactat
     6541 cttcttttgt aactatagaa attgcaccac tatccaaaat atctcttata acttgctgtt
     6601 gaggaagtat aagtctacct tttggagctg ctttatctat tggaacaact aaaacaatta
     6661 aatcatttgg ttcaattaaa tctgatacca atttgaactc agtgctatcc ttaggcaaaa
     6721 cttttattat ttcatttttt aaattttcaa ttccaatttt atctgttgat gagacagaaa
     6781 caactggaca tttaacttta tttttagtta agtttaaaat ttctgattga ttcttaatag
     6841 tatcaatttt gttgagaatt agaatatgag ggatattctt atcattaaac ttttcaatta
     6901 gtgacaaatc ttcttgtgat ataccaatct ggcaatccac tactagtaaa gctatgtctg
     6961 tcttttctaa aacatctagt gatttaccta ttctaagttc accaagttca ccaacatcat
     7021 caagaccagc agtatctata agaacacaag gtcctatagg aaggatttcc attggtctaa
     7081 atactgggtc tgtagtagtt ccagcaatat ctgagactat tgcagcactt tgatttgtta
     7141 tagcatttat tatgctagat ttacctgcat ttcttttacc aaagagacct atatgtactc
     7201 ttaccgattg tggagtagaa tttagactca tttgtactca ccaccttaaa cttctttttt
     7261 tgatatacta gtttttacag atacatgagg tatgttgccc aatttaccag tcaaactatt
     7321 aatagtatcc atttcgccaa caactgctat acatacgatt gagacacctt cttcttcgca
     7381 aggaatgccc attcttcctt taataatacc tttaaaacct gatacaacct cattgaattt
     7441 aaattgacat tcttttggct cttccaaaat agcacttata actgcaacct ttttcataaa
     7501 tatcctccta actcagaaaa tatgtcttaa acaaatccaa aatcaaaaaa cttttctttg
     7561 taaagaaaag tttctacaaa agaaaagtta ttaattacac aagtataatg tgctatatta
     7621 aactatttcc ttacagatta gataactctt ttctggtagg gttagcttgt aaaaatttaa
     7681 tttaacaaca agtaactgaa gaaaattttt atacaattta tataaattat aataaaaatt
     7741 aactaaagaa aattgaagtt acttatataa tagtagtaaa ttgaaattaa gtctatagaa
     7801 aaaccaattt ttgtataaaa ataaaaatta aagttaatta tttgttaaga attgactttg
     7861 gtaaaatata taattaatta ttctttaagc atataattta taagtaattt tagtgtattt
     7921 tcaatagctt ctatatgtgt tctttcataa tggtgactat tgtttgttcc aggaccaaca
     7981 catgctattt gaacatcttc tccccaacga atagcttctg aagcatctga accatagaaa
     8041 gtaaatacat cgacagcgta gtctaaatta ttgtcttctg caacatcaac aagcttgttt
     8101 cttaaattaa aatcatataa tgataaatta tcttttgcaa ctatagttac accatgttca
     8161 ctagatgttt gacttggtcc agtaggtgct atatctattg ctacaaattc ttttgttttt
     8221 tcaggtaaac atttagaaac accatgtcca atttcttcat aattacttat ataaaagtta
     8281 gtagtatgct taggtgttaa attgttttct ttaaaatatc tacatatttc aagaaccatt
     8341 gctacagctg atttattatc tatatatcta gattttataa aaccagattc tgtagcctca
     8401 aatctatggt ctaggcatac aaaatcccca acacttattc ctaaatctaa tacatcttgc
     8461 tttgtaaata ctttttcatc aagtctaata atcattgttt cttcatttct ttcagtggca
     8521 gcttttgttt gaccatatat atgagtagaa gcatacttaa atacaacact tcctctgatt
     8581 tttttacctt ttctagttat aacagtacag ttttctccct ctatagctgc ccaacatcca
     8641 ccaccaactt tatggtattt aagagtacca tcagatgata tttcttttac tatagctcca
     8701 agtgtatcca tatgtgcaga tattgttatt tgatttgaat catcttcacc aggaagagta
     8761 gctataagtg cacctttttt tgtaagaata gtgtcaagac ctaaattttc aaaatctttt
     8821 ttgatttcta aaactgcatt ttcagtatat ccagctgggc ttggaatgtc caaatatttt
     8881 ttcattaaat ttatagtatt agttaaattt aaattcatat tgtttcctcc taaataaata
     8941 tatttataca aattataaca taaatacaaa tgttctgaaa a
//
LOCUS       DACULQ010000154         8867 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.155, whole genome shotgun sequence.
ACCESSION   DACULQ010000154 DACULQ010000000
VERSION     DACULQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.155"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            231..1310
                     /locus_tag="JA154_14790"
     CDS             231..1310
                     /locus_tag="JA154_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997110.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="JA154_14795"
     CDS             1634..2002
                     /locus_tag="JA154_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HAT7997111.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDNDVPQWRLDKHIGKPIK"
     gene            2110..2922
                     /locus_tag="JA154_14800"
     CDS             2110..2922
                     /locus_tag="JA154_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7997112.1"
                     /translation="MNTMEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLF
                     DFQIQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSA
                     GGFVAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNI
                     ISKMCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDK
                     DKTGKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3307..4197
                     /locus_tag="JA154_14805"
     CDS             3307..4197
                     /locus_tag="JA154_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT7997113.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVFKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
     gene            4760..5203
                     /locus_tag="JA154_14810"
     CDS             4760..5203
                     /locus_tag="JA154_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7997114.1"
                     /translation="MIEIKTIEINKKSQIDLLILADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5375..5545
                     /locus_tag="JA154_14815"
     CDS             5375..5545
                     /locus_tag="JA154_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997115.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5918..7039
                     /locus_tag="JA154_14820"
     CDS             5918..7039
                     /locus_tag="JA154_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997116.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTQKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            7244..7732
                     /locus_tag="JA154_14825"
     CDS             7244..7732
                     /locus_tag="JA154_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997117.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7818..8225
                     /locus_tag="JA154_14830"
     CDS             7818..8225
                     /locus_tag="JA154_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997118.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            8226..8444
                     /locus_tag="JA154_14835"
     CDS             8226..8444
                     /locus_tag="JA154_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997119.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
BASE COUNT         3505 a          855 c         1565 g         2942 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagaacgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tgctacctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtacgg taaaaataaa tctactaact
      901 ggattgtaaa gcatggttgt agaactctac taaaaaaagg aaatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagct atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttagcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaaag gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggataa tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtttttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgatgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaacaa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaattaca tactcattag
     2941 gtaaattatg tagtatatgt gtttatagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat ataaattttt
     3061 aagcgaaata catcaaaaga agtattagaa ttagtggaga taaaacagct attagagatt
     3121 ataaataaat agttttaaag gtactacgaa tatagaggaa ggtaaatcat acgttattca
     3181 aggattataa atgattagtt taaaagtact atcggacagg tctctgcata tttaaacctt
     3241 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     3301 aagaaaatga ttgagagtat accagcaaaa caaattttac aaaaagtgaa atttgacaat
     3361 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     3421 atatattgtg atagccgtag cacaatatac aacatagaga attttgacaa agttagatat
     3481 aaagaaaatg tgatagagat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggggta
     3541 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     3601 aagcaggcac tagatttgat ttcggaaaat cattttggag tgtctattga tactaaaagt
     3661 agtttggtgg ttagagatat accgattttg cagaaaatta aaaaaaataa ttctgctatt
     3721 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     3781 aatccaagtt ctgtacgatt tgaggcagtg aaagaactta atgattctgg aattttttgt
     3841 ggtatattat tgactcctat gcttccattt ttgactgata ctaaagatga aatacgagca
     3901 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgtatgta tggtgtaaca
     3961 atgcgtagtg gtcagcgaga gtttttttat gagcatttaa gagatatatc accaaaattg
     4021 gtatttaaat atcagaaaac atatggttta aactatgtat gtactgtaca aaataaagat
     4081 tgttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     4141 gatattataa aagcgtacaa gaggtcggag tcatatactc aaataaaatt tttttaagat
     4201 aggctttgga tgagatataa tagtggtgat tgatgtgttg atattatagt tgattgttag
     4261 agaggatatt atagatgaat attagtatta atattttagg taattgctag agctgatatt
     4321 atagatgaat attaatattg atattctagg taattgctag agttagtatt atagatgaat
     4381 attagtattg atattttagg taattgctag agctgatatt atagatgaat attaatattg
     4441 atattctagg taattgctag agttagtatt atagatgaat attagtattg atattttagg
     4501 taattgctag agctgatatt atagatgaat attaatattg atattctagg taattgctag
     4561 aactaatatt atagatgaat attagtattg atattctagg taattgctag agctgatatt
     4621 ataatttatt attaatatta atattctagg gaattatcac tatattttaa aaagtaaaaa
     4681 tatatatcaa aatgaactaa ataagtactg taagaagttt gttaaattaa ggtttgaata
     4741 aagtgagggt ggagaactta tgatagaaat aaaaactata gaaataaata aaaaaagtca
     4801 aattgacctt ttaatattag ctgacccatg tgaagaaatg attgataaat atctcaataa
     4861 aggaactatg tacgcattgt atgataataa agaacttact tgtatagcag ttgtaaatga
     4921 aatttccaaa gaaatatgtg aattaaaaaa cattgctaca tatgagcatt ttcaaaatat
     4981 gggttatgcc tcaaagatga tttatcatct tttagatgtt tatagtaaaa aatattcatc
     5041 aatggttgtt ggtacatctg aaagtggagt tcctttttat gagaagtttg gatttgttta
     5101 ttcacataaa attaaaaact tttttgtaga taattatcca gaaccaatct ttgaaggaga
     5161 attacaatgt gttgacatgt tgtatctatc atataacttt tagataggat aatattttta
     5221 ggttaatata attaattaat tcatataatt atcggaatta taatttataa attatgtata
     5281 ataagagtat aatttattta gaagctgtta tattgtatta gattcaaata agatatataa
     5341 taacttgttt attttgaatt ttggaggatt aattatggat tcaaataata atactataaa
     5401 atcaactgtt aaaaagggta tttcttttgg ttcttgttta gcaatgatta tttcttatac
     5461 tgcatggaaa tctattccat gggctatttt tcatggctta atgagttgga tatatgtact
     5521 ttattattgg gttaagtatg catagattaa attaataaat aagtatgtaa tattgtaaag
     5581 tttaagaaac aaaatgtaag ggagtgtctc gaagtgaact tttagtttat aaggcactct
     5641 tttttgtatt aaataaaata tattttcatt acttaaacaa tgaaaaagat gagtatctct
     5701 attttaatac atacccttat attcattcat ttatcaacaa tagttttaaa aagaaagcta
     5761 tctaaaatct attgtaatct aagtgcttct ctttaaagtc ttaactttag taatgatata
     5821 taagtttata aggttcttat ttcaataatg caaattttac ttagaagttc agactttggt
     5881 atgaagaaat gggtaataat aaaaaagagg tgttgatatg aaagaaaaat tagaagaaaa
     5941 atctaagatt attaaaacat caaaagagta taaacgaact ataattgaag aaaaggacag
     6001 agaatggacc caaaaattaa atgacatact tagtagaggt gatttttcta atgaaaaatt
     6061 agataaaatc aaggatttaa ttccaaaaga aatattgact agtgaaaatg ttggagaagt
     6121 tgtaactgaa gatgggtatg ctaagcctga gtatgatgag gtatttaaat cattgtttac
     6181 tttaaataat gattacgatt tacttgcaaa ttttataaat gatatcctca aagatgctcc
     6241 atatgctaat aggaatatca aacagttcac tcaaataaaa agaataatta aggttgaaac
     6301 cgacccaaca attaattaca ttggtgagaa aagacctaga cttgatattt tggctgaaga
     6361 tgaagaacgt aaccatataa atattgagat gcaaagagct cttgaagagg attatttgga
     6421 aagagctgaa tattatctta gtcgtgtaca tggtagaaag ttagaagaag gtaaagaata
     6481 taaagaaatt ggaaaaactg ttggaataca tatactaaat catgtaaaat ataatcacat
     6541 agaagattat gtaaattgcc tgagattaac aatggacggg catccagata ttttttctag
     6601 taaaactgca ttatatttta tagaacttcc aaaaattcgt aaatccagtt gtatagcaaa
     6661 cagagttcta atatggggta aacttattga taatccttct catatagata ttagaatact
     6721 atctaaaacg gattatgtta ttaaaagagc acttgataga ttaaaagaat taggtagtaa
     6781 tgaggactat ttattaaatt taaagagagg tgcgtatatt atgaataaaa gtagaaattt
     6841 taaagaagaa atttttcaag aaggtattga aaaaggtatc gaaaaaggta tcgaaaaagg
     6901 tattgcaaaa gggaaaagag aagagaaatt taatatcatc ataaaattaa aaaataaagg
     6961 atacaatttg agtgaaatat gcgatattat agatgatttg aataaatcag aggttgaaaa
     7021 agtctataat caaaactaat actatgattg agatggcact agataaatac aaagcttcct
     7081 cttttaaatt taaattaata taaaactttg ttatttaaat atggtagata taaagagata
     7141 tataaataat aggtacaaag aggtgtataa ataaaggaga taattcaaaa taaaagtttg
     7201 aaccatctcc ttcttataga aaaacaaagg ggttgattta tatatgaaat tttatactat
     7261 acagacttta gaattttgga ataacaataa aaacaataaa tatctatcaa atgattattc
     7321 ttttatatta gaagactttg atattccata caaatggatg tatagtcaaa tgacaaatag
     7381 aatatatgaa tttgatgata gtatgatatg ggtttggacg aagagacctg atttaagatg
     7441 tagtggttat ttagaaaaag gaaaaaaagg tgttttactt gaaatagaaa taagtgaaga
     7501 caatgtgtta ttatcagaat ttgatttatg gcatttacct ttaatggata ttactgtaga
     7561 gctttatgaa gatgaacaaa ttgaaaagaa ggtaagttgg gaaagaatat ttgacttaga
     7621 actgtgttca aaattaaatg aagttaatat agaagatttg acattgcaag gagtgacatc
     7681 taaagtgcct ctatctaatg taaaattaat aaaagaattt gttgcaaaat aatattttta
     7741 ttgaaattta ttcaaaaaag ttatatattt ataacaagag gtgttataaa tacataactt
     7801 tttaaggggg attttatatg tctaaacaac aaactggtat taaatcaaca cttatttcag
     7861 aaatattggg aatttcttat tgtattggag ctacattagt aaataagtat ttttttaaag
     7921 gaagtagtat tgaatatctt cctataatta tttgggtatt agctatggga gctcagtggg
     7981 tctataataa aaagtatgat gatttaattg atgaggcatc aaaatgtata ctatcaagag
     8041 ttaatgatat tgcaataaaa gtattgtttt tttctgtagc tatagtatct atatttcttg
     8101 taactttgga cataaatgtg tcaaatttaa atataggaat gtctttatta gtcattttgt
     8161 ttattcaatc cttgttgaag ttaattctat ttatatattt tgacagaaag gggatataca
     8221 attaggtgtc agtattacta aagacaaaaa ttaaatccta tagagaaaag aaccttatga
     8281 cacaaggtga acttgcaaag tcagtaggag taagacgtga aactattgta catttagaaa
     8341 atggtcgcta caatccttct ttaaaactgg caatggatat tgctaaggta tttgatacaa
     8401 ctgtagaaga gttgtttgaa tttattgaag aaaataatat ttaaatttca tgtattactt
     8461 atgcattaga gaatgaatta attataggta aaaaaccaag cttatagatt agagaataaa
     8521 tgacttatag atgaaaaacc aagctaatgg attaaagagt gaatgattta tagattaaga
     8581 agtaagctta tagattagag aataaatgac ttatagatga aaaaccaagc taatggatta
     8641 aagaatgaat gatttataga tgaaaaagaa agcttataga ttagagaata aatgacttat
     8701 agatgaaaaa ccaagctcat agattaaaga atgaatgatt tatagatgaa aaagaaagct
     8761 tatagattag agaatgaatt aattataggt aaaaaacaag ctaatagatt aaaaaatgaa
     8821 tgatttatag attaagaagt aagcttatag attagagaat aaatgac
//
LOCUS       DACULQ010000155         8592 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.156, whole genome shotgun sequence.
ACCESSION   DACULQ010000155 DACULQ010000000
VERSION     DACULQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.156"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            76..2253
                     /locus_tag="JA154_14840"
     CDS             76..2253
                     /locus_tag="JA154_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HAT7997120.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFTNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2267..2689
                     /locus_tag="JA154_14845"
     CDS             2267..2689
                     /locus_tag="JA154_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT7997121.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3097..3900
                     /locus_tag="JA154_14850"
     CDS             3097..3900
                     /locus_tag="JA154_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT7997122.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            3893..4561
                     /locus_tag="JA154_14855"
     CDS             3893..4561
                     /locus_tag="JA154_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAT7997123.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            4797..5459
                     /locus_tag="JA154_14860"
     CDS             4797..5459
                     /locus_tag="JA154_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAT7997124.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5519..6316
                     /locus_tag="JA154_14865"
     CDS             5519..6316
                     /locus_tag="JA154_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT7997125.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            6449..7624
                     /locus_tag="JA154_14870"
     CDS             6449..7624
                     /locus_tag="JA154_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7997126.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSKLIKEE
                     LNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILEYIV
                     KNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDYLCQL"
     gene            7733..8482
                     /locus_tag="JA154_14875"
     CDS             7733..8482
                     /locus_tag="JA154_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAT7997127.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         3409 a          860 c         1586 g         2737 t
ORIGIN      
        1 taaaatctta ttattaaaaa tattcttaga aattacacta tataaaatat aaagtatgat
       61 atttgaataa aaagaatgta taataagtat aataatttgt atttattatt gggaaaggaa
      121 aatgttaata tggatagtta tagacaactt aaagctaaaa taccctgtaa ggaaacaggt
      181 atagaaataa agaggacttt atgtgatatt tgttctccag gagtacattg tggaattgat
      241 gcatatgtta aagatggtaa agtaataaaa atagaaggta caaaagaaca tccaatgaat
      301 aaagggttgt tatgtaccaa aggtctttgc aatagagaat atatctatag agaagataga
      361 ataaaaacac ctctaaaaag aattggaaaa agaggtgaag gaaaatttaa accaatatca
      421 tgggatgaag cttatagaga aatagcagaa aaattaaata gagtaaaatc tgagtatgga
      481 gcaaagcatg tagctttttt tacgggttat ggaaagtggt atagaccttt tttacaaagg
      541 tttgcatctt catttggttc acctaattat ggtacagaat ctagttcatg ttcaacatca
      601 gcacagatgg ctggaaatgt tgctactgga agaaacttta caatgcaaga tatggagaat
      661 gcaaaaactt atttagggtg gggatttaat ccatactact ctaaattttt aaatagtgtg
      721 aatctaataa aattaaaaga aaaaggtcta aaatttataa ttgtagaccc tagaataaca
      781 ccgacaactc aaaaactagc agatttacat ttaagaatta aacctggaac tgatggagct
      841 ttagctcttg gaatggcgaa tctatttata caaaaggatt taatagatag agaatacata
      901 gaaaagcatg tacatggatt taatgaatat aaagaatatg taaaattatt tgatttagat
      961 actgtaagta gaattacagg agtttctaaa gataatatag taaaagctgt cgagatgttg
     1021 tcacaaggtc cattttgtat aaatgaaagt gcagctccaa tagttcacca tcaaaatgga
     1081 tttcaaaatt atagagcaat gatgtcttta tccgctataa caggtaatta tgatagaata
     1141 ggtggaaaca taccaaaaca agattacttt gctggtaaga taggtggatt ccaaacacta
     1201 gaaaaagagt ttgtaaaaga agttctacct aaaaatataa atgaaagaat tggtggagaa
     1261 agatttcctc tatggaatga aatggtgact caatttcaag ctatggattt acctaggcaa
     1321 atagaagaaa agacaccata tgaaataaga gctttgtttt gtcatgggat aaatgctaga
     1381 atgtttacta attcagggag actttttaaa gcgttgaata gtgtagattt ttttgtgaat
     1441 atagatttat ttatgacaga tacatcaaaa tatgcagata tacttttacc tgcatgtaca
     1501 tcatttgaga gagaagaatg taagagttat ccaggagggt atattacata taccaaaaaa
     1561 gttattgata aattatacga ttcaaaatca gatgtagaaa tactttcaga tttagcaaat
     1621 gctatgaata tagatgatga tttattaaaa gcaggttatg aagcaagtat aagacatatg
     1681 tttaaaaata catgtgtaga tgtagataaa cttaaagaaa gtgatttacc attaaaatgt
     1741 aaagatttta agccatatat agtaggttca tatacaagag aagggtatga tactagcact
     1801 tctaggtttg aattaaaatc tacaattatt gaaaaatata aagattttgg gctggatgca
     1861 ctaccaacat atagaggatt taatgataat atagatgatg aatatactca tttattgaca
     1921 tcaggagcaa gaatttcaaa tgcactacat tcaagacttc atgacatcaa actttcaaga
     1981 atattaaaaa atgaagctag ttgtgatatt tctagcttag atgcaaaaga attgggtgtt
     2041 atcagtggag attatataaa aataactact aagataggaa gtttggatgt aaaagtcaat
     2101 gttactgata gagtcttata taagaatata catatgtatc atggatatag agaagctgat
     2161 ataaatagta ttatttgtga taattttgac ccttatagtg gattccctgc gtatagaagt
     2221 tgtaagtgta agattgaaaa aatgaataga taaatggaga gatataatga aaagtttatt
     2281 taaatttaat aaagaattat gtacagcatg tggagcatgt ttgcttgcat gtatagacca
     2341 aaatgataat aatatagaca atggtgaact ttttagacat gtatttacaa tagaggaagg
     2401 tgaaaatata gaatattttt cattagcatg tatacattgt aatgagccaa aatgtttagg
     2461 tgtatgtcct aaaaattgtt ttaaaaaaga agaaggattc attgtattag acaatcaaaa
     2521 ctgtataggg tgtaaacttt gtgaaaaagc ttgtgaatat ggagccttaa tttatggaaa
     2581 tgatggtaag gcaaataagt gtaatggttg tattgagaga tataagtgtg gtatggattc
     2641 accttgtgta atggtatgtc ctacaggagc attaaaattt aataaataaa ttaaatatag
     2701 agaatgactt atacttaggt ataagatatt tttacaaaaa gctgtattga gtttgttata
     2761 atgaaaactc aatatagctt ttttatggat atagttggat actataatgt gattcttagt
     2821 aatagttttt tgctattgga caaagactgt aaaacatatt aaacttatgt tgttcaaagt
     2881 cgaagtataa tataataact agaaaattgg tttatagtgc tataaaagca aatttagtaa
     2941 ggtataaaat cagaggcaat cctttactat aatatcaata tttgggagtt ctgttattgc
     3001 agagctatta aacaattata atggtacaag tgattcaacc tttgaaaatg tattatataa
     3061 aaaattaaaa taagattaaa aaggagagaa taaattatga aaaaacttaa attaattatt
     3121 atgtttgcct tggtatcagc actgttagtt acaggatgtt catcaggaaa taataaaaat
     3181 aaagaagaac aaaaagttat aagagttgca acatcaggaa catattaccc ttttgctttt
     3241 aaagataaag ataaacttaa agggtttgaa gtagattttt gggatgaatt tgctaaacga
     3301 actaattgta aagttgaatg ggtattaact gattttagtg gattatttgg attattagaa
     3361 gctgataaag cagatgttgt atctgctcaa cttactgcaa cacctgaaag agagaaaaaa
     3421 tatgcttttt cagatgctta taattatagt ggaacaaaca ttattgttag agaagatgat
     3481 actagtataa aatcaataga tgatttagtt ggcaagaaag taggtgtggg tactggagca
     3541 gtagcaaatg aaatattaaa agaaaaatac ccaaatgatg aaataaaaat agtaaattat
     3601 tcaagtgcga ctcttaaagg gaattatcaa gacctagaaa tgggaagatt agatgcagta
     3661 gttgcgcagg atgttgaggc tttaatagct ataaaagaac aaaaattaaa tcttaaaatg
     3721 atagaacctc caattcaatt tggtgcctgt tcttttgcgc ttcaaaaaaa tgaaaaaggt
     3781 gaaaaatgga caaaagaaat taacgaagta attgaagaga tgtataaaga tgggactttg
     3841 actaaaatat ctgaaaactg gctaggaaaa gacattacta aggagcctat taatgagtaa
     3901 ttttaatttt gaattttgtc taagtatatt tccatttttg attaaagcat tgggaataac
     3961 aatactaata tctattgttg catttttttt atcaatatta ttaggtgtaa tacttgcctt
     4021 tttcacacag acaaaatcaa aagttttaca atacataaca agagtgtatg tttcattctt
     4081 tagaggaaca ccacttttaa ttcaaatatt tttcttttat tttggatttc caatgatatt
     4141 ttcttttatg aaagattgtt caccttattt agcaatagta atttgtttag gtgtaaattc
     4201 atctgcatat atggctgaaa ttatacgagc atctattgag agtgttgata aaggacaaca
     4261 ggaagcatgt tatgcttttg ggttaagtaa atatcagtca atggttaaaa taattctacc
     4321 tcaagcagct acatctgcag tacctcctat tgtaagctgt acactggaca taattaaagg
     4381 ttcttcttta ggtttaacta taggaataca agatttaatg ggagtagctc aagtagaggc
     4441 tgcttcaagt tttaaatatt ttgagatata tatgataacg atattaattt actgggcagt
     4501 atctataata ttgagtaata tacaaagcaa agttgaaaaa agaataagaa gagcatatta
     4561 gatttcaata tttaaaaatg ggatgatata gatatattat tacttgtaaa taaattttta
     4621 attttagaaa aataaactta gtaatctaaa ttattgagaa aatatagtat caaaatggac
     4681 tgtataatca agttaaaaaa tagtttttct attgaaaaaa taaataaaat tctattgtaa
     4741 tttaatatgc gataaagttt tataaaaaac aacattaagc tagggggact tattttatgg
     4801 cttttaatat gaaatatttt ttagaattat ttccaacaat aatgaaatcg ttcaatgtaa
     4861 ctgtgatttt ggcaataagt tcattagtat tttcattaat tataggtaca atagtagcgt
     4921 tatcatctta ttacaaagtg aaagtattga atcaaatatg taaagtatat gtatctattt
     4981 ttagaggtac acctcttatg ccacagttat tctttttata ttttggacta gcatatatga
     5041 gtgattttgt taaaaatatg gacccagtat ttgcaacatc tatagtactt agtttaaata
     5101 tgggagctta tatgtctgaa acgataaggg catctatact ttcaattgat acaggtcaga
     5161 tagaagcagc atatgcaatg ggattaacaa atttacaaac aatgaaaagg atagtgatac
     5221 cacaggccgt aagagttgca ctgccatcat tattcaataa ttttatagat ttaattaaag
     5281 ggtcttctgt agcttttgtt gttggagtaa atgatataat gggtgctgcc aaatcacaag
     5341 gagcgttgag ttttaagttt tttgaagtgt atggagcagt tatggtaatt tactggtgta
     5401 taataactcc tttaggttgg gggcaaaaaa aattagaaaa gaaaataagc aattattaag
     5461 ttattaagaa aatacataaa agtattttta tataaaaaat ggtgtgagga gaagagatat
     5521 gataaaaaga aatattataa aatgtttagt ggcagtttta tcgttaacgt ttgtaattac
     5581 tggttgttca aaaaaagagg aagtaaagaa tgaaaaagtt ataactgtag cttcatctaa
     5641 ttcagctgac ccatattctt atgttgataa tggtgagcat aagggttttg aagttgacat
     5701 gtggaaagag attggtaaaa gaagtggata taagataaaa atggaaccaa caggttttag
     5761 ttctatattt ggtatgttag attctggtaa agttgatgtg gcagccaact tctttggaat
     5821 gtctaaagaa agattagaaa aatacgatgc ttctatacct tatggttctg attctgtgtg
     5881 tatagctgta aaagatggaa ataacgaaat taataagctt gaagatttaa agggaaaagt
     5941 tgtagcagtt tcagaaggtt ctcaaggtca acaggttgca actagtgtta ataaagataa
     6001 tttgtttcaa gaaaaggttt acggtgatgg aactagtggt cttcaagatt tagatttggg
     6061 aagagttagt gcatggattg atgcagagct tactattgtg ggagacgcta aaaaagctaa
     6121 tatgaaagta agagtattag ataaaaaatt atcagctact aatatagcat acttctttaa
     6181 gaaaaacgat gataaatcta aaacgataaa agaagatgta aacaaagcaa tagaagaaat
     6241 gcttgcagat ggaacagtga aaaaaatatc tgaaaaatgg tttggaatgg atgtaagtgc
     6301 tgatatacaa aaataaatac tttaaaatta aaatgtaaaa ctgtgataac tatagataag
     6361 tatactaaat ttatctatag ttatctgtgt attacaatgt atttagatta gtctttataa
     6421 atcaaaatga gttatggagg tattgcaaat gattgaaact aaaataaaat ccattgcact
     6481 aaaaaataga gaaaaaatta taaaaataag acgacagata cacagtaatc cagagttagc
     6541 atttaaagaa tataaaacat ctaaacttat aaaagaagag ctaaataaac ttaatataga
     6601 atacataaat attgctggta caggagtatt agctactata aaaggaaaaa ataatggtgg
     6661 aaaaactata ttactcagag cggatatgga tgctcttcca atcaaagagg aaaatgattt
     6721 agaatttaaa tcaataaatg ataatatgca tgcttgtggg catgatgcac atgtttcatg
     6781 gttgttagga acagcaatga ttttaaatga cataaaagaa gaattgaatg gaaatgtaaa
     6841 attactattt caacctggcg aagaaaaagg tggttctgat attgtaatta aagaaaatgt
     6901 acttgaaggt gtggatgcat tagcgactgg tcattgttgg ccaactattg agtcaggaaa
     6961 aattggaatc gctagaaatt gtgctatggc agcgactaat acttttgaaa taacaataat
     7021 tggcaagggt gggcatggag cagagcctca taattgtata gacccaattg cagttggaaa
     7081 tgcagtatat tcatccattc aacagatagt ttcaagaaaa attgacccag ttgtacctgt
     7141 agtagtgtct gtttgttctt ttaattctgg tgtttctaaa aatataatac cagatgtatg
     7201 tacacttcaa gggactatta gagcgataag caaggaacaa gtaattgaag taagtaaaat
     7261 tctagaatat atagtaaaaa atgtatgtaa agcacatgga gcagattgca aatttgaaaa
     7321 gagtacagga ggagatgctg ttataaatga caaaaacatg atagagatag gaagagaaag
     7381 tgcttgcaaa atacttggag ctgaaaatgt tgaaataatt gattttcctg caatgacagg
     7441 tgaggatttt gctatttata tgaaagaaaa acctggttta tttatgtata taggtgtagg
     7501 agataaagaa aaaaatataa attacagatt gcatagtaac aagtttaata tagatgaaaa
     7561 atgtcttagt atagcacctt cattattttc acaacttaca gtagattatt tatgtcaact
     7621 atagagtaca ttcataaaaa tatatttagc ctatttttaa atttaatgct agtatattaa
     7681 ttttgatgta gaacatatta tttattatat ttatttaaaa aggtggataa caatgataaa
     7741 agtagaaaat ttacataaaa gttttaaagg gacagaagtt cttaaaggta taaatttaga
     7801 gataagtcaa agtgaagtag tagctatact tggatcatct ggaacaggta aatctacgtt
     7861 acttagatgt ttaaattatt tagttgagcc tacaaaagga aagataatga ttggtgatgt
     7921 aaaagtagat gtagaaaaag caacaaaaaa agatataacc aatttaagaa aacatacatc
     7981 tatggtgttt caaaattata acttatttaa aaataaaaca gcattagaaa atgtaatgga
     8041 ggcattgata gtagttcata aaaagagtaa gaaagaagct agagaaatat ctttaaaatt
     8101 aatagataaa gtaggtatgt tggataggaa agatttttat ccatcaaagt tgtctggggg
     8161 acagcaacaa aggataggta tagcgagagc gatggcagta aatccaaatg taatattatt
     8221 tgatgagcct acatctgcac ttgacccaga acttgttgga gaagttttaa gcgttataaa
     8281 atctcttgca aaagaaggta taacgatgct tattgtaact cacgaaataa attttgcaaa
     8341 agaagttgct gataaaatta tatttatgga taatggtgta atagctgaag aaggaactcc
     8401 tgatgaaata tttaataatc ctaaaaatga aagaacagca aaatttttaa atatcattag
     8461 gaaagaagaa gtatttagtt aaaagtgtat aaattaagca ttagaagata tgtaagatac
     8521 ccccattaaa tttataagtt agataataaa tttaatggga gtatattttt attattagat
     8581 ttattattta at
//
LOCUS       DACULQ010000156         8573 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.157, whole genome shotgun sequence.
ACCESSION   DACULQ010000156 DACULQ010000000
VERSION     DACULQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.157"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            149..2752
                     /locus_tag="JA154_14880"
     CDS             149..2752
                     /locus_tag="JA154_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAT7997128.1"
                     /translation="MSVGLDTVNNVESLVKKLAKIREAQKIFATYSQEQVDKIFLAAS
                     LAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAYKETKTCGVIEKDEAFGM
                     TKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSPHPRAKNSTIEAAKVVLE
                     AAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGMVKSAYSSGKPAIGVGAG
                     NTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLENIYNEVKKEFKERGAYL
                     LDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPVDTKVLIGEVESVEIEEA
                     FAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLYIDDVNQREKLDKFTSAM
                     KTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVGVKHLLNIKTVAER
                     RENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTDTFLYNNGYTKAVTDLLD
                     EMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGGGSAMDAGKIMWTLYEHP
                     DVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGSEVTPFAVITDQDTGVKY
                     PLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEAYVSMLATDYTNGLALQA
                     IKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFLGVCHSMAHKLGAFHHVP
                     HGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAEIASFLGLKGNSDEEKFQ
                     SLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAFDDQCTGANPRYPLMNEI
                     KDMYLNSYYGR"
     gene            3028..5247
                     /locus_tag="JA154_14885"
     CDS             3028..5247
                     /locus_tag="JA154_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HAT7997129.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFVAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            5501..6163
                     /locus_tag="JA154_14890"
     CDS             5501..6163
                     /locus_tag="JA154_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HAT7997130.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            6534..7943
                     /locus_tag="JA154_14895"
     CDS             6534..7943
                     /locus_tag="JA154_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAT7997131.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            8058..8333
                     /locus_tag="JA154_14900"
     CDS             8058..8333
                     /locus_tag="JA154_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997132.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
BASE COUNT         3397 a          885 c         1536 g         2755 t
ORIGIN      
        1 aaatgttaaa aaaataactt gaaaatagga aaagtaaatg ctataatgtt aataaattaa
       61 cataataaat aaaaaactat ctcaggaggt tgttaaaatg gctcataaag aacaagtaat
      121 aatagaaaat aaaaaagaaa atgaagttat gtcagttggt ttagatactg taaataacgt
      181 tgaaagctta gttaaaaaac tagcaaaaat cagagaagct caaaaaatat ttgctacata
      241 tagtcaagag caggtagata aaattttttt agcagcatct ttggcagcta ataagcaaag
      301 agttcctcta gctataatgg cacaaaaaga aactggtatg ggaattgctg aagataaggt
      361 tataaagaat cattatgcct cagagtatat atataatgca tataaagaaa ctaaaacttg
      421 tggtgttata gagaaagacg aagcatttgg tatgactaaa attgctgaac caataggtgt
      481 tattgcagca gttgtaccta caactaatcc aacttctact gctatcttta aagcactttt
      541 agctttaaaa actagaaatg gaataatttt ctctccacat ccaagagcaa aaaattctac
      601 tatagaggct gcaaaagttg ttcttgaggc tgctgtttta gctggagctc ctgaaggtat
      661 aataggatgg atagatgagc catctttaga attaactaca actgttatga aagaagtgga
      721 tttgaccctt gctacaggag gtcctggaat ggttaaatca gcatattcat ctggaaaacc
      781 agctattgga gttggtgcag gtaatactcc agcaataatt gatgatagtg cagatataaa
      841 aactgctgta aactcaattc ttgtatctaa gacttttgac aatggtatga tatgtgcttc
      901 tgaacaatcg gttatagtat tagaaaatat atacaatgaa gtcaaaaaag aatttaaaga
      961 gcgtggtgct tatttattag ataaagatga aacagaaaaa ataagaaata taattttagt
     1021 aaatggagga ttaaattcta aaatagttgg gcaaacagct tgtactatag caaaacttgc
     1081 aggctttgaa gtcccagtag atactaaggt tttaataggt gaagtagaat cagttgaaat
     1141 agaagaagct tttgctcatg agaaactttc accagtactt gcaatgtaca aggcaagtga
     1201 ttttgatgat gcagttagaa aagcagaaaa attagtagaa gatggtggat ttgggcatac
     1261 atcttcatta tatattgatg atgtaaatca aagagaaaaa cttgataagt ttacttcagc
     1321 catgaaaact tgtagaattt taattaatac tccttcttct caaggtggta taggggactt
     1381 atataatttt aaactagctc cttctcttac tttaggatgt gggtcttggg gtggaaattc
     1441 agtatctgaa aatgtaggag ttaagcattt acttaatatt aaaacagtag ctgagaggag
     1501 agaaaatatg ctttggttta gagcaccaga aaaagtttat ttcaaaaaag gttgtttagg
     1561 agtagcatta aaagaactta aagatgtaat gaataagaag agagctttta ttgtaacaga
     1621 tacattcctt tacaataatg gatatactaa agcagttact gatttattag atgaaatgaa
     1681 tattaagtac actacattct ttgaagtaga accagaccca actttggaat gtgctaagat
     1741 aggcgctaaa gctatgagag aatttaatcc agatgtaata atatctatag gtggaggaag
     1801 tgcaatggat gcaggaaaga taatgtggac attgtatgag catccagatg tagatttcca
     1861 agacctagct atgagattta tggatataag aaaaagggta tatacattcc ctaaaatggg
     1921 tgaaaaagct aattttgtag ctataccaac atcagcaggt actggttcag aagtaacacc
     1981 atttgctgtt ataactgacc aagatacagg agtaaaatat ccattagcag attatgaact
     2041 gatgccaaat atggctatag ttgattctga tatgatgatg aatatgccaa aaagcttaac
     2101 atctgcttct ggtatagatg ctttaactca tgcattagaa gcatatgtat ctatgcttgc
     2161 aaccgattat acaaatggat tagctttaca agctattaag agtatatttg aatatcttcc
     2221 acgtgcatat gataatggtg caaaagaccc agaagctaga gaaaaaatgg caaatgcttc
     2281 tacaatggca ggtatggctt ttgcaaatgc atttttgggt gtttgtcact ctatggctca
     2341 taaattaggt gctttccacc atgtaccaca tggtgttgca aatgctcttt tgataacaga
     2401 agttatgaaa tttaattctt cagatgcacc aaagaaaatg ggagcattct ctcaatataa
     2461 atatccagaa gcattaaaga gatatgctga aatagcttca tttttaggat taaaaggaaa
     2521 ttctgatgaa gagaaattcc aaagtttatt ggtagctatt gaagatttaa aacttaaagt
     2581 tggtattcct aaatctataa aagagtttgg cgttgaagaa tctaagttta tggattcaat
     2641 agatgaaatg gtaattcaag catttgatga ccaatgtaca ggagcaaatc caagatatcc
     2701 acttatgaat gaaataaaag atatgtattt aaattcatat tatggaagat aagtttgcta
     2761 taaaatagat aaaataaaaa ctcttataca taaaacaaag ttttattgta taagagtttt
     2821 tttgtttgag aaaatggata gtttttattc tttaatttaa aattcaatta aatagtgtta
     2881 ttataataat aaaagaatgt ttttatggtt attatgttga ttttatgtgt aacatttaag
     2941 aatagttata tacaatataa atgatgaatt ggtataaaaa ggatatatta tctatatgaa
     3001 gtattgacta attaggggag gagtcgaatg acagggagaa ataaaagttt tacattaatt
     3061 acaattcttt ttttaacatc attatttttg acaatggggt atatctatat agaagataca
     3121 aaacatcttc ttatgtcaga ggcaaaaata catataaaag aagttgcagt acaaggctct
     3181 caactagcac aaagacaaat tgaaaaagat ttagatgttt taaatatttt ttctaactat
     3241 tatgcttcaa atccagatat acctaatgag gaaaaaatga aaaatctttt agatgaaatg
     3301 gaaaatcaaa aattttacac tatggcaata gttgatataa atggtaatgc tgaaaatacc
     3361 aaaggtgata agttttcagt gaaagataga gaatttttca aaaactcaat taaaggtaag
     3421 aagtatgttt caagtccata tgttgatgaa gtaaataagt ctataaagaa gattgctatt
     3481 tcagtaccat tattaaataa tgataaagta gttggagttt tatattgtac ttacaatatc
     3541 aatactctga tgaaacttat taatatatct ttttatgaaa ataatagtat atcttatgtt
     3601 gtaaaaaatg atggtacaat tatccttcat ccacaagggg atagcctatc aaaaaatatt
     3661 tataaattgt tagaacagga taatgatatt caagaagtaa atagattaaa aaaagaattg
     3721 caagaaaata aaacaggtgc aactgtatta aatatgttgg aagaaagaag atatttaggg
     3781 tatgccacta tggataatgg gaatagtgaa aataattata taaaagactg gaatttaatt
     3841 ttttcaattc cagaaactgt tgtatttagt aattcaaaac aaataatcaa cagagctgta
     3901 tatgcagtgt tatctattgt attaatattt gttgcaatta ttttttatat aatttatata
     3961 aaaaaatcta atgaaaaaga aatattaagt ttggcttatg aagataaagt tacatatata
     4021 ggaaatcaaa ataaatttta tagagaatgt agtaagtatc ttttagataa accatcttta
     4081 aattatatta tagtatattt tgatattaat aactttaaga tgattaatga tacttttgga
     4141 tatgaatttg gtgacaattt attaattaca atagcaaaag ctttaaaaga agaattaaca
     4201 gaaggtgagg tatatgcacg actttcaagt gattattttg caattttctg tgattataaa
     4261 aatggaagaa ataaaattat aagaaaactt gataatatac gaagtaatat tgaaagtaat
     4321 ttgagtatag tctttgaaat ttctctgtgt gtgggtattt attttgttga agaaggtgaa
     4381 gtagatattc aaaaggctgt aaataaagca aatatggcta ggtctgttgc taaaggtaaa
     4441 aatataaatt atgctattta taatgaagat gttaggaata agcttagtga ggaatcaatg
     4501 attttagacg atataaaaat agcattggta aaaaatcaat ttgaagtgta ttatcaacct
     4561 aagttttcac ttgtaactgg agaaatgata gggagtgaag cactaataag atggaatcat
     4621 ccagaacatg gttttataag tcccgcagta tttattccaa tagcagagaa aagtaagttg
     4681 attttaaaaa ttgggagatt tgtatttgaa agggtttgca atgatttata tgaatggaaa
     4741 aagcaaggta aaaagatagt tccagtttca gtaaatttat ctagggtcga gttgtatcaa
     4801 ccagatattg taaaatttat caataaaaca ataaaaatgt ataatttaag ttcagatttc
     4861 attgaaatag agataacaga aactgtggct attaatgagt taaatatttt aaagaatgtt
     4921 ttaaatgagt tgaggacgta tggattttct atttctatgg atgactttgg tacaggatat
     4981 tcttctatta gttgtttaag agatatgcca attgatatat tgaagctaga taagtctttt
     5041 cttggtggca ttgaacatga tgagagaagt cgcaatatag caaaatcaat agtgtctcta
     5101 gcaaaatcac tagatttagt tgtaattata gaaggtgttg agagtaaaga acaagctgag
     5161 ttaatgaaac aatttggatg tgatttagtt caaggatttt attttgcacg accaatgcca
     5221 gctaagaatt ttttagattt actttaaaac aaaatttgat ttttatataa agcactatta
     5281 tgaatgcttg attttaataa atcattgcta aataattagt gtttttttat tttatcaatg
     5341 tattgtctaa tataaagcaa aaaataattg tattttataa aaattatatt aaaaataaat
     5401 ttattttgct aataaacatg ttattaacaa aagaatagta ctataatgta tagatgataa
     5461 aaactttaat aaaaatttag tgtataggag ttgtttaagt atgatagata taagtattga
     5521 tgacaaactt aaggaaagat ggcctaatgc taagctgggt tgcattcaag caaaagtagc
     5581 agtaaataaa agctcagaaa aattaattaa tgaaataaat gagttttgtg atactataaa
     5641 tcaaagtcta aaagttgaag acattacgaa actagataaa attaaagatg caagaaatgc
     5701 ttataaagag cttggaaaaa atccaagtag atatagaaca tcttcagaag cattggttag
     5761 aagaattatt caagggaagg gtctttatac aataaataat atagtagaaa taaacaattt
     5821 aatatcaata aaatcattat atccagtagg tacatataat gtaagcaaat tacattcacc
     5881 agtatgtttt actgtaggag atgaaggaga acagtataag ggtattggga aagaattaat
     5941 aaatatagaa aatcttccta tattgtctga ttcagtgggt aaatttggga gtccaactag
     6001 tgattctgaa agagcaatga taacagaaga tgcaaatgaa atattaattt gtatattttc
     6061 ttttagtcaa gaatctgata ttgaagaata ttttggatat gctaaggaat tacttaaaga
     6121 atatgcagat ggaaaagata tagaaactaa aataattaaa tagaatctat ataacaacgt
     6181 tatgttaagc taaaattcaa tataaaaata agttaagata aagaaactaa cacaaaatta
     6241 gtttcttttt taaaatagta gtttagtgtt acaaaaatag atagatagtc ccacgattat
     6301 taaaagtatg gtaatatatt aatatagcag agattgtgtt taagggattt tgaaaggggg
     6361 gatttataat aatcatttta tctaaaagat aaaatggttt tatagaaata actatatatt
     6421 taaaaatata aataatgata cagtaatttg ggtatataaa ataaggcata catggttttg
     6481 tttaaaaacc aataaggtac ataggtaatt acaatgtaag gaaggattta attatgattg
     6541 atggaaaaga ggtaaaacaa gaaagtggac aaaaaaatct aaaatttaag atagctatag
     6601 tagtaggtgt tttgtttgta gcttatatag gaatggcaat cttttttaga aatcattttt
     6661 actttggaac gactattaat ggcgtaaagg cttcaggaaa aacagttgag caagtaaatg
     6721 atttgttagc atctagtact aattcctata ctttaaactt agaggaaaga aacaataaga
     6781 aagaacaaat aaaagcaaaa gatattgatt taaaatttac accagatgat aaagttcaaa
     6841 agataaaaga tagtcagagc gctattggat ggattggtgg aatatttaaa tctaaagagt
     6901 ataataatat gataactctt tcatataata aagatttact agaaaaaagg tttaatgctt
     6961 tatcttgttt tgatagtaaa aatgttgtag aacctaagag tgcttatcct aagtatgata
     7021 gcactaaaaa cgcttatgtt atagtaccag aggtacttgg aaacaaagtg aaaaaagatg
     7081 atttttataa gttagtacaa aactcaatta attctgggaa tacttctatt tctctggatg
     7141 aaagtaaagt ttatgaagca ccaaacaaca caaaggattc agaaaagttg aaacaagcta
     7201 ttaagacatt agataagtat gttggtgtag tcgttacata tgattttagt gaccgtaagg
     7261 aaactttaga tggttctgaa attaataaat ggttaaaagt agactcagaa aagaattatg
     7321 acataacttt tgataatgat gctatggtag aatatacaag aggtttaagt aaaaaataca
     7381 gtacttttgg tgactcaaga ccatttaaaa ctgccacagg taaagatatt acaatttcag
     7441 gtggaattta tggttggttg atagataaaa agaaagaagc tgaagcatta gttgatgttg
     7501 taaaagctgg taaaaatgtt acaagagaac ctatttacat acaaaaagct gtatctcgta
     7561 ataaagatga tattggaaat acatatgtag agattagtct ttctggccaa catatgtggt
     7621 tctttaaaaa tggagaaatg cttgtatcaa cagacgttgt aacaggtgat ttagctagag
     7681 gatttgctac accatcaggt atatatcctc ttaattataa ggctagaaat gtttcattaa
     7741 ctggtcaagg ttacagttca cctgtaagtt attggcttcc atttaatggg aacataggca
     7801 ttcatgatgc tacttggaga aattcttttg gtggaagtat atataagtct tcaggttcac
     7861 atggatgtgt aaatacacct ttttctaagg caaagaaaat atatgaaaat atagaaccag
     7921 gtacaccaat tattttatac tagtaaatgt gttgctaaca gttaagcgaa gtcatcctag
     7981 tttatttagg atggcttcct ttttatataa agcttattga agtttacaga aagactactt
     8041 aaaaggagaa atacataatg aatgaaaaat taactaaaat tttagaagaa gctaagaatg
     8101 atgatgaatt tagaaaggaa cttataaaaa caagagaaca acgtgatgtt atggattcat
     8161 tttgtaaggt tgttacatct agaggttatg atatgacagt aggggaactt ttttctatgg
     8221 gagaagaata tacatccaat ctactgaaaa gtgtaaatgg aggagcagca tatcctattg
     8281 aagattggag tgattggtac gaggattttt ttataatgtt gtcgaatata tagagtaact
     8341 aatgatatta tctacttaag attaatacat tgccttaaaa taaaactatt agaactaggt
     8401 gtaagtttta tcaatcttta aaaatattga tttatttcta actagatata aaaaaattag
     8461 attaaaaata aggtgacaaa aatcgatata gcttgatgat ttataaaagt tagataaaat
     8521 agtgcgagat atttctaaaa attcaaaaaa aacaataaac ttcaatggtt aga
//
LOCUS       DACULQ010000157         8258 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.158, whole genome shotgun sequence.
ACCESSION   DACULQ010000157 DACULQ010000000
VERSION     DACULQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.158"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            147..599
                     /locus_tag="JA154_14905"
     CDS             147..599
                     /locus_tag="JA154_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT7997133.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTNEVKNASDASHLVKYITNKMKEE"
     gene            602..910
                     /locus_tag="JA154_14910"
     CDS             602..910
                     /locus_tag="JA154_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAT7997134.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            1024..2130
                     /locus_tag="JA154_14915"
     CDS             1024..2130
                     /locus_tag="JA154_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAT7997135.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            2205..4883
                     /locus_tag="JA154_14920"
     CDS             2205..4883
                     /locus_tag="JA154_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAT7997136.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPTSLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(5200..7521)
                     /locus_tag="JA154_14925"
     CDS             complement(5200..7521)
                     /locus_tag="JA154_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7997137.1"
                     /translation="MKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGAENPF
                     EQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTYDGSE
                     TYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLDTTKN
                     DASKENSNENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYGVNQK
                     IINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGYGNTY
                     FDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLSRIPL
                     EILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLTYWFV
                     IFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKAVINQIRTINLKEKPN
                     KTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLLDVTK
                     KISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVSHDLK
                     TPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGDVYLN
                     IVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLIINIS
                     KYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEGSGLG
                     LAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(7508..8224)
                     /locus_tag="JA154_14930"
     CDS             complement(7508..8224)
                     /locus_tag="JA154_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7997138.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         3092 a         1164 c         1150 g         2852 t
ORIGIN      
        1 tttatttcca cttctatatg gaaattagtt attatcataa tataaatttc catgtcgaag
       61 tggaaattag ttatatttaa tttaattagg ataatgatta caatataaat ataagttaag
      121 acattatgaa tggagtggaa aaatatatgg tactacaaga aaaatatata tttatagata
      181 aagacttaaa aagcaaagaa gaagtattaa attttattgc agatagtgct tatatactag
      241 gtattacaac taataaagag gctcttttaa aagaccttct aaaaagagaa aatgaatttc
      301 caacaggaat aaaagatgga tttgcgatac ctcatacaaa aagtgtaaat gtaaaggaag
      361 cttccatact ctatattaaa acaactaacg agttagagtg gaaaacttta gatgatagta
      421 aggttagata tgtatttagt ttgttagccc cacataaaaa tgaaggaaat atacatttga
      481 aaactctatc taaattagcc acatgtttaa tggaggatga ttttacaaat gaagttaaaa
      541 atgctagtga tgcaagtcat ttagtaaaat atataactaa taaaatgaag gaggaataat
      601 tatgaaaata gtagcaataa catcatgtat agcaggatta gcacacacac ctatggcagc
      661 aaaagcttta gaacaagctg catcaaaaat gggtcatgaa atcaaagtag aacaacaagg
      721 tgctatggga cgagtaaata ctataacaaa agaagaagca caagatgcgg actttatact
      781 tatagcatct gaccaaacta tatctggaat ggaaagattt gatggtaaaa aggtgataaa
      841 agtcaaaata ggtatagcgt taaaaaaacc agaagctgtc ttaacaaagt gtatagaagc
      901 tatttcttaa taaaaaaatc tcactctaat atagaatgag aaaaatcaaa aattaaaaaa
      961 ttgtctaatg taattataac atattatcaa aaaatataac aaacttatat aggaggatta
     1021 aaaatgagta aaaaaaataa atctctttta agtgaactta agggacactt aatgacaggt
     1081 atctcatata tgttgccact tgtaataggc tcatctttag ttgtagctat cccaaagctt
     1141 acagctttag caatgggtat aacaagttta gatgcttata aaaatgtatc tggtttttat
     1201 catattcttt acttaatgga acaagttggt tggactggca taggtctatt aaatacggta
     1261 ttagcaggat ttatagcatt ttcaataggt gagaaaccag ctatgggtgc aggttttatt
     1321 ggtgggctca tagcaagtaa tactaatgca ggattcttag gagcagtaat agcaggtttc
     1381 tttgctgggt atgtttgtaa gtttttaaag aaaaaaatta aaatatctgg gtcagcatca
     1441 ggaatgatgc cacttataat aatgccactt ataacagttg gattaactgg ttttttaatg
     1501 agtgttgtat tagcaggacc tttaggaaac ataaatactt cattaaatgc ttgggtagct
     1561 gaaatgtgtc aaaatggaac aaattcggtg gtattggctt taattttagg cgcaatgata
     1621 ggattcgatt taggtggacc agtaaataaa gcagcttgga tggcaggaaa tgcattattg
     1681 ttagagggaa tctatctacc agcgatattg gtaaactgcg caatagttat acctccacta
     1741 ggatatggtt tagctacatt cataagaaaa tcaaagtttt ccaatgcatt agctgaaaca
     1801 ggaaaaggca atatagttat gggtataatt ggaataacag aaggtgcaat tcctttcact
     1861 ttaacaaatc cattaaaatt agttcctgtt aatatgatag gatgcgcttt gggtgtagga
     1921 ttatcagctt tgttaggtgt acatgctata atgcctccag ttggtggatt atatggattt
     1981 atatcaatag gttctggatg ggcttactta gtaggtgcaa tatttggagc tttagtaata
     2041 gcaatattat caacaacttt agtagacttc aatgaagatg aaactacgga aaatacagaa
     2101 tttgatgaaa tagagttaga tataatgtaa attaaaaact taattttaga taattgagaa
     2161 ttgagtgtac tctcaattct caattaaatt ttgggaggat ttttatgaaa aagacaaaag
     2221 tacatgtagt tccacacagt cattgggata gagaatggta ttttacaaca tcaaggtcaa
     2281 aaatatattt aatgaaagat tttcaagata tattagacat attggaagaa aaagaagact
     2341 ttaaatactt tactatggat gcacaagctt ctctcttaga tgattattta aaatggagac
     2401 ctcaggatga atatagaata agagatttag ttcaaagaag aagattagtt attggacctt
     2461 ggtatactca aacagaccaa ttggttatat caggagaatc tataattaga aacctttact
     2521 atggaataga tagatgcaat gaatttggtg aacctatgag ggttggatat gtaccagatt
     2581 cttttgggca atcagctcaa atgccacaaa tatacagagg ttttggaata gatagttcat
     2641 tattttggcg tggagtttct gatgatatgg tagatacaac agagtttata tggaaaggtt
     2701 ctgatggttc aaaagtatta gcagttcaaa taccatttgg ttattactat ggaggtaata
     2761 taccagaaga tgattctgat ttaaaagaat atttaaaaga tataatcgga aaacttaagg
     2821 ttaaagcttc tactaataac gtatattttc caaatggatt tgaccaagca ccaataagaa
     2881 aaaaccttcc tgatatatta aaaaaagcta atgaaataga taatgaaaat gaatacgaaa
     2941 taagtgatat agaaaactat atagattcag taagaaatga aagaaatgaa tttacagagc
     3001 ttaaaggtga atttttaaat gctaaacata tgagaataca caaatccata ttctcctcaa
     3061 ggtcagacct taaaataatg aataacaaat tagaaaatta tattacaaat gtacttgaac
     3121 cattattaac attaagttat tcacttggaa atgaataccc tcacctagtt ataaaagata
     3181 tatggaaact tatgtttgaa aatgcagctc atgacagcat aggttcttgt aatagtgaca
     3241 caacaaatga ggatgtatac acaagataca aacaagccag agatttgagt ataaatttat
     3301 tagatttgca tatgagactt ataagtactc aaatcaaaaa taataatgaa gaaataacat
     3361 taacagtatt caatggattc ccagaagtca gaaatgaagt tgtagaattt gatacttata
     3421 ttccaggtca agattttgtg ataaagaata aaaatggaaa aattcttaat tatataataa
     3481 aagaaaaaga agatattaca aattatatat taactcaaac aataagatta aacccaagta
     3541 aaaaaatata tatacccaaa actgtatata gagcaaaaat tgcactttca gtaaaagata
     3601 taccaccaat gggatatact caactagttt ttgaattgga tactaaaaat aaatgtgaaa
     3661 tagaaacaag taattgcaag gaaatagaaa acaaatacta caaaataaca gccaataaaa
     3721 atggttcatt agaaatatta gataaagaat ctaataagac ttataaaaac caagctgtaa
     3781 ttgaagacaa tggagatgat ggagattcat acaactattc tcctccaaga aaagatattt
     3841 atgtttcatc tttagattct gtatcttcag tagaagtgct aaaatcaaac attcaagaag
     3901 aaatgatact taaatattca ttgaccatac caacttcact ggaagaaagg gctataggta
     3961 aagtttctgt gaaaatgcca gtaacaatga aaatcacctt agaacctaaa gaacaaataa
     4021 ttaagtttaa tgttaatata aaaaataacc aagcactatc tcatagagta agagttttat
     4081 ttaatacaga aatagcatct aagttttcta tagcagatca acaatttgga ataatacaaa
     4141 gaccaacagt attagaaaaa gaccttgcat tgtggaaaag agacaattac tcatggcaag
     4201 aaaaacctat aactatcgaa ccaatgcaaa gttttgttac tttagaggat ggacaaagag
     4261 gaatagcatt aataactggt tgtgtaagag aatatcaaat agttggtgat aatttagata
     4321 caatagcatt aacactattc aggtcatttg gatatatggg aagagaaaat ttactttata
     4381 gaccaggaag agcttctgga gaaaaaataa tagcaacacc agatgcacaa ttacttaaag
     4441 agttaaactt tgactttggt ctttacatat ataacaataa atttgatgaa gcaaatgtag
     4501 ctaatacagc taagagattc ttaacaccta tacaaatata tgaatatgct gattttttaa
     4561 atggtagact tatatttgca ttcaatgacg aaaaacaaat atatgaaaat gaatttagtt
     4621 tgatgaatgt aaataattca aatttcacag taagtgcaat aaagaaagaa gaaaaaggag
     4681 atggaattgt agttagaatc ttcaatggta tgaaaaatga ggatgctaag ggaagtctta
     4741 acatcaataa aaatgtcaat gatgcttata gtgcaatgct cgacgaaatc tatgataata
     4801 ctagcaagct taaagtaaat actaaaactg tagaggtaaa ttctttatca cattgtaaag
     4861 tacaaacaat agtaattaaa taatataaaa tagcttatac aagatttcaa aataaattgc
     4921 tagtactatg ttaatagtaa agccagatat tatgctattt ataagctctg taacactaaa
     4981 taacattttg taaaataaaa aggtgaacct tgtatattag gattcacctt ttttaaataa
     5041 atcaacattc tgttttaaaa taacatttat aagctgtttt gaaataatgc ttacaaatta
     5101 ttttaaaaca atacctataa atttttattt aaagaataca cataaatatt tccagattct
     5161 tttattctat agactttata attattaaag aagtctaatc taatttttgt taaaagtaat
     5221 tgtaactttg aataaatcac catccacact tatattcatc ttaccaccct gtaattctat
     5281 aaaacttttt gctatcgcta aaccaagacc agaaccttct gtgttacgtg atttatctcc
     5341 tcttacaaat ctctccatta tatcatctac actaaaatca atctcagatg cagacatatt
     5401 tttaagcata atatttactt tattatctgt ttctaaaata tcaatataca ctcttgaatt
     5461 tggcattgca tatttgctta tatttattat aagattttca aatactctaa atgtacgctg
     5521 actatctaat ggtaatatta ttttttcatt agaaaagtta ttctttacta ttagagatga
     5581 atctgcaagt ttgtcatcta attctaaaag tgtctgcttc attagagata caatatccac
     5641 atttactata ttcaaataaa catctccact atttgccttg cttacttcaa acaaatcttc
     5701 tattagatgt tgcaatcttt gtgactttct gtctaatgta tcaatataca acttacgatt
     5761 ttcatctgtt atactttcat cttttaataa atctatataa gtgattatag atgttagagg
     5821 tgtttttaag tcatgtgata catttgatat aagctctgtt ttcatctttt gactcttaac
     5881 ctcttcgtca acagcttttt taaatccagt ttgaatattt cctaattcat cttttatagg
     5941 attaaaaaat cctaaatctt tttccatatt tacatctaag tttccatctg aaattttctt
     6001 tgtaacatct aaaagttgat tatattcata tattgtcttt ttagagtaat ctgacagtat
     6061 cttaaataat actactgaat atattaatgc gagtattatt ccaaagaacc acatactaca
     6121 cattataact ataatcaata aatttattcc caaaattgta actatagttt tatttggctt
     6181 ttctttcaga tttattgttc taatttgatt aataactgct tttcctgctt tttttaacca
     6241 tacaaaaaac attcctgtca cagtatgttt tgtaaaatac tctcttattc caacatttaa
     6301 aatgtgtttt aaaagtatta taaatacaaa tattattgag aatattacaa accaataagt
     6361 taaattcatt aaacttacca atatactagc cattttagca cttatgtcat tgtttataag
     6421 gtactctaca taattcccac tcaaggtatt acttattata acagtaggat ataaatctac
     6481 aagcccctgt ataatagcaa gagcaataaa gagtatctct aacggtattc ttgataatgt
     6541 atcaaatcct agcaactctt tagattttct atatgggaat gctaaagcta caataacaac
     6601 tatagctaat ataattttta aaatcattcc agaaatctta taataagaat tattttctac
     6661 attgcttata tatgaaatta taccatcatc aaaataagta tttccataac catactctac
     6721 actatcacct ttacttatta ctttaggtac agcatatact aatgttatat tttttattgg
     6781 agataactta tatgaaattg aatttcctcc taaacttgta tccattaaat cttctcttgc
     6841 tttagataac aacatttgat taataatttt ttgatttact ccataagaat ccacaatatt
     6901 tacttttcca tctttatcaa aattcattac aacgtagaat ctatagtttt cttttatatc
     6961 tgtaatctgc ttttgtcttt cctcatcatt catttctgta ttttttgact gattttcatt
     7021 agaattttct tttgaagcat cattttttgt tgtatcaagt actcctaatt ctttattatc
     7081 attaaaaaat tcaaaattgc tttctttatc aactaaatag tactttaaat tctttcctga
     7141 cgctttaatg aaatgctccc aattttcaaa gttttcatta aaatctgcat taatactatc
     7201 atctgtataa taagtttctg aaccatcata agtatctttt tctttactta cattttttat
     7261 atagttcata gtttcttcat tgataaatag ctttgatggt tgtatcatct taccttcttg
     7321 ttcttccttc atattatgat ataaaacata acttccttta tatatactat ctacaaagct
     7381 ttcctgttca aatggattct ctgctccacc tttagcactt gcttgtatag tagaatattg
     7441 actaatcata cctaatgatg ctgataacat aagtataaaa actataatag taactatata
     7501 tttatcttta aattttttca attttgtatc caacacccca caccaccttt aaatatttag
     7561 gattttttgg gtcaatttct atcttttccc ttatattcct cacatgtacc ataactgtat
     7621 ctgtatttac agcattctca ttccaaactc tttcatatat ttcatcagca gaaaaaactc
     7681 ttcctgcatt tctcataagt aaacttagta ttttaaattc tataggagtt gtttttatat
     7741 actttccatc aatactaact tcttttgtat tttcatttaa ttctaatccg cctattgcaa
     7801 acacactatc gtcactttct accttttgct ctttatttcc aaccatgttt agatacttag
     7861 tgtaccttct aagttgtgaa ttaactctag ccaatagttc taatggctta aaaggttttg
     7921 ttacataatc gtctgctcct atattaagcc ccattatttt gtcaacttct tctgattttg
     7981 cagatagcat tattactgga aaattatagt tttctctaag cttcattgta agtgttatac
     8041 catccatttt aggcatcatt atatcaacta tagctaaatg tatttcttgg ttttctataa
     8101 ccttaagacc ttctaatcca ttgtatccct taaaaacatt atatccttga tttaatagat
     8161 atatttctat agcatctgct atctcctttt catcttctac caccaaaata ttatatgaat
     8221 tcattctaat ctcctttata ttaatttata aattttct
//
LOCUS       DACULQ010000158         8200 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.159, whole genome shotgun sequence.
ACCESSION   DACULQ010000158 DACULQ010000000
VERSION     DACULQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.159"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            85..1071
                     /locus_tag="JA154_14935"
     CDS             85..1071
                     /locus_tag="JA154_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAT7997139.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKKLKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     EMSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1240..1385
                     /locus_tag="JA154_14940"
                     /pseudo
     CDS             1240..1385
                     /locus_tag="JA154_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter"
     gene            1407..2888
                     /locus_tag="JA154_14945"
     CDS             1407..2888
                     /locus_tag="JA154_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 32"
                     /protein_id="HAT7997140.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            2914..3885
                     /locus_tag="JA154_14950"
     CDS             2914..3885
                     /locus_tag="JA154_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAT7997141.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4141..4743)
                     /locus_tag="JA154_14955"
     CDS             complement(4141..4743)
                     /locus_tag="JA154_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT7997142.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKSVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILSNSIELDNC"
     gene            5024..6235
                     /locus_tag="JA154_14960"
     CDS             5024..6235
                     /locus_tag="JA154_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HAT7997143.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6303..6500
                     /locus_tag="JA154_14965"
     CDS             6303..6500
                     /locus_tag="JA154_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAT7997144.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6534..7505
                     /locus_tag="JA154_14970"
     CDS             6534..7505
                     /locus_tag="JA154_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7997145.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFRNALWMYKFTGLFIKDKTK"
     gene            7692..8069
                     /locus_tag="JA154_14975"
     CDS             7692..8069
                     /locus_tag="JA154_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT7997146.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3325 a          865 c         1328 g         2682 t
ORIGIN      
        1 tattgactgg acaaataact atattttatt ttaaatatat aatataatgt aataaaatat
       61 atcaaatgta gaaggtgata acctatgaaa aaaataacaa taaatgatat tgctaacttg
      121 gcaggagtat ctaaaagtac tgtatcaaga tatctaaaca ataaagatat cagtgattct
      181 actaaagaga aaataaagac aataatagat gaatatggat atgagccgaa tgcatttgct
      241 caaagtttga gagctaaaaa aacatatttt attggtatta taacaccgtg tttagattct
      301 tttgtaaaat ctaaaattat gatggcaata gatgaagaac tcaaaaaatt aaaatataca
      361 tcactaatta ttaatactaa tagaaaaata aggtcagaaa tagatagtat atcaaagtta
      421 gctagtctaa aagttgatgg aataatacta attggtaccg aaataactaa agaacataag
      481 aatgaaattg aaaagttaga tataccgata gttgtagttg gacaaaaagt tgatggtatc
      541 aatagcatag tgaatgatga ttatggagca ggctataaaa tgggtcaata tattgctaat
      601 aaaggttaca aaaacattgt atatttaggt gttgatgaaa gtgatatatc agttggttta
      661 aatagaaaaa atggagtact taatgggtta aaagataaag ggtatgatgc aaaagtattt
      721 tatactgact ttgaccaaga aatgtctata caaagaagtg gagaaatgct agaaagtgaa
      781 aatccagata taataatatg tgcaactgat aatatagcaa tagcaacaat gaaagaaatc
      841 aataagagag gtaaaagtat accacaagat atatctgtag ctggatttgg gggatatgat
      901 gtattgtcta ttataagccc taaacttaca acaataaagt ttgaaaataa aaatgcaggt
      961 aaagtagcag caaatacgat agtaaattta atacaagaaa gaaaagaacc actattaaag
     1021 gagattaaat ttgaattaat agaaggtgaa agtacaataa ataaaaatta agatacacat
     1081 tacatatatt agtaatgtgt atttttttac taaaaaaact ataattctaa aaattaggaa
     1141 aacttttttg aaagaaatat tgcatctgaa ataaatttat gctaatcttt aattaaaccg
     1201 gtttcgaaac cggtttaatt aaaccgatag aatagcaaaa tgagtgcaaa aataactgat
     1261 tgtaacaaag tatcaaagta attattggag tacataggaa taaacaaaat ataaaagatg
     1321 tagcacattg tgtaataaga ctaagaattg ttttagatag caataataaa gcaaatataa
     1381 aattataaaa gaaaaggaat aaaaatatgt acaaagagcc taaatataga accatattag
     1441 aatcgacaaa agaagaatta aataaattaa gaaatatatc tttaaatgat aaatataaac
     1501 cactatttca tatacatcca caacatggac tacttaatga cccaaatgga ttagcatatt
     1561 ataatggtaa gtaccatgta ttttatcaat ggtatccata tgatgcaact catggaatga
     1621 agcattgggc ttatgtaagt tctgatgatt ttgtaaactg gaatagagaa gatgtagcat
     1681 taataccaat cgaaagttat gaatctcatg gagcatattc aggtaatgcg atagaggtag
     1741 atggaaaatt gcatatgtat tatacaggga atataaaata tagtgcagaa gatagatatg
     1801 catatcaaaa cttagctata atgaacaagg atggtaaaat aactaaatat gaaaataatc
     1861 caatagtaag tgaaatacca aaaggatata caggacatgt aagagaccca aaagtattta
     1921 aaagaaagga taaatacttt atgttacttg gagcacaaac aagtgataaa aaaggagtta
     1981 taatagtata tgaatctaaa aactctatag attggaattt taaaggagaa ttaaatgtaa
     2041 aaaacataga tgaagatttt ggttacatgt gggaatgtcc agattatatt aatatagatg
     2101 aaaaagatat attaatattt tcaccacaag gagtagaacc taaaggattt gattatcaaa
     2161 atatatataa tgtagtatat gctattggta atatggattt agataattta acatttgaaa
     2221 tagatacaat gaaagaactg gaaaaaggat ttgatttcta tgctccacaa acatttataa
     2281 aggactcaca aataatacta tttgcatggg ctggaatggg agaagtttta taccctacag
     2341 ataaaaacaa atgggcacat tgtctaactg ttccaagaaa attaaatata aaaaataata
     2401 agctattgca aatgccagta gatgaattaa ttaaattaag atatgatgaa acaagtgggc
     2461 aaaatactat aaagaataat attaatatta tagaaaatga tgaaaatgta tatgagctta
     2521 atattaatat aaaaaatatt gacagcaata aatttgggct ggaattattt tcatcacaag
     2581 atgaaggtgt aaaattagaa tttaacaaat taggaaacat tgtaacttta gacagaagca
     2641 actttaaaaa agttttcggt gttgaatatg gaacaaatag aaaagaatat ataaatattg
     2701 atgaaaatac taatatcaaa gtactagcgg atagaagtat tttagaaatt tttataaatg
     2761 aaggagaagt agtatttact agtagaatat ttgctaaaga aaattctaat caaataaggg
     2821 tttatagtga taaaatagta gagtatgagt atactaaatt taaattaaag caaggtattg
     2881 aattataaaa acacattata aggggtagaa aagatgaaaa aagtaattag tataggagaa
     2941 gctttaatag actttatacc taatcaaaat ggtggtaaat taaaaaatgt atcagaattt
     3001 agaagagtag caggaggagc tccagcaaat gttagtgctg ttgtagcaaa gcttggagga
     3061 aaatcgagtt ttatatctaa gttaggaaaa gatgcatttg gtgattatat aatagatgta
     3121 ttaaatgaag taaatgtaaa tactgattat gtattgagaa caagtaaagc aaatacagga
     3181 cttgcttttg tatcattaaa agaagatggt aatcgtgatt tctcatttta tagaaatcca
     3241 agtgcagata tgcttttaga agctgatgaa gttaaaaaag agtggtttaa taattgtcat
     3301 atcctacatt tttgttcagt agatttaata gatagtccaa tgaaattagc acataaaaaa
     3361 gcaatagaat acgcacttga aagtaatagt ataataagct ttgaccctaa tataagatta
     3421 cctttatggg atagtgagca atcttgtaaa aaggcaatat cagaattttt accttttgct
     3481 catattgtta agatatctga tgaagaatta gaatttataa caggagaaaa tgatatagaa
     3541 aaatcattac ataaactctt tgtggggaat gttgaactag tattatatac aaaaggaaaa
     3601 gatggagtag atgcatacac taaaaaagta aaaggtatgt gtaaaggggt taaagtaaat
     3661 gcaatagata caaccggagc aggagattct tatataggtt catttttata caccttattg
     3721 tataaacaaa taacattgaa tgatataaaa gaaatgaaac aagaaacgtt aaatgagtat
     3781 ttagaatttt ctaattatta tgcagctcgt agtacaacta ttaaaggtgc gataagttca
     3841 tatgcaacaa aggaagaaat aactgaattt ataaaaaatt tatagtagga atgagtaata
     3901 ggttttgaag gaagtaacat gtaaagagtt tttaaggatg tttactatga aaataattaa
     3961 catataaaaa gcaataagga ggctgtctca aaatagagat aagcctcttt atcattgttg
     4021 aaatgataaa aaatatttta attattgtta ctttttagaa gaatatatat cttattttaa
     4081 ataagcaaat tatgtaagat ttatttatat tatcaatact ttacttggag tattatgtct
     4141 ttagcagtta tctaattcaa ttgaattaga taaaatataa taaatttgtt ttgcaaattg
     4201 acgagaatgt tcttcttttt ctttattcat aaaattaggt ttacctaaat cacgttcaac
     4261 aagcattcct atatatttta actgtgaatg cttacagtag cgcttcatac cattttccca
     4321 aatatcagca cccttttcag gtctatatcc acatgttgta ataagtgcta ctgattttcc
     4381 tgcccataga gcggggcctt tctcattacc atagtattta ttcattccat acaccaatct
     4441 gtcaagtagt gctttcattg gagaagtgca gtaccaagaa taaattggag tagcaaaaac
     4501 aattaaatca cacataagaa ttttcttgaa aattttctga acatcatctt tacaataaca
     4561 tccaaaatca aataagttat cctgacactt tttacaagca atacatggct ctattttaca
     4621 atcatataac catattactt caatatttga attataattt tctaattcct caataaaagg
     4681 ttttaaaata gcagcagtat tacctttttt cttaggactt cccataagaa tgcatacttc
     4741 catattgata cctccacagt attttaaaat agtttttagt gcttaatata gttttaggat
     4801 actatgagaa tacgttaaca acaatacact ttgtatattt gtaataaaat tgaaacaaat
     4861 tgttttatca ataaaagttg gaatgtatct ataattaaaa atattttagg tatgttttta
     4921 catatatgtg tgtagttaac tatggtaatt tattgtaaaa ttatcttata ggattataat
     4981 tgcttaattt atgattatat ttaaaatctt ggaggtaaaa aatatgtact caaatgaaga
     5041 ctataaattt atagaagaaa atgcaccatg ttatatatat gatgagacta atataattga
     5101 aaaatgtaag gaattgaaaa ataatatttt aggttttgat tttttatatt ctataaaaac
     5161 aaatccatat aacaaaattg taaaaagcat tgcagagcaa ggttttggag cagatgcttc
     5221 ttcaactaat gaagttatga tttcatcaaa atgtggagtt tctaaagatg atatttatta
     5281 ttcatcacca ggcaaaacag agattgatat agaaactgtt ttagataaat gcataattat
     5341 tgcagatagt attacagaaa tagaacttat aaataagata tctaaaaagc ataatcagaa
     5401 agttaaaata ggaataagaa taaatcctga ttttactatg gatgatgata gtgctaaatc
     5461 aagcaaattt ggtatagata tagaagatat gagcagtgtt tttaagatgt taagtatgtg
     5521 tgattatgta tctgtagttg ggattcatat acatattaaa tctcaggtat tggattttga
     5581 aaagttagga cattattatg aaaaatgttt ttttatagct ttaaatttaa ataaaagtaa
     5641 gtatattaat attgaattta taaattttgg tagtggactt ggagtagctt atgataatct
     5701 tagagaaaat ccacttaatc ttaaagagct ttcaaaatta actcaaaaga ttgcttttac
     5761 taattcaaac acactaaaat caagacttct tattgaatca ggacgtttca tagtttgtaa
     5821 atcaggtaat tattatgtta aaattgtaga tattaaagta tcaagaggga aaaaatatct
     5881 tattgtaaaa aatggactta atggatttat gagacctatt atttctaatt taattgaaaa
     5941 tattacttgt ggagtagaat tagaaggtca agagccatta tatacatcaa atcatgcctt
     6001 ttctctttca gtattaaata atttaaaaga agaagagtat gtaaatgttg ttgggaatct
     6061 ctgtacatca ttagatgtaa ttgcaaataa tgttaaattg aaaaaagcaa acataggaga
     6121 tttgatttca attagtaatg ctggaagtta ctcctattct ttatcaccgc tattattttc
     6181 aagttatgaa tttcctaaac aatattataa aagaaaagat ggatatattg agtaaacaat
     6241 agcataaatt aataaactta gtattattga aaaaatttaa aatgcagggg gtgtaattct
     6301 atatggaatt gtggaaaaga aatttatttg tatgttggat aggtatgttt tttagttcta
     6361 tagggatgag ccaaatagca cctatacttc cattatatat taaacagttg ggggtaactg
     6421 atgttagttt aatacaacag tattcaggta taatttttgg atgtaccttt gtagtagcag
     6481 cattcttttc gccaatttga gggaaagctg ctgataaata tggaagaaaa tcaatgcttt
     6541 tacgtgcaag tattgggatg ggaatagtta tatttaccat ggcgtttgtt caaaatgttt
     6601 atcagttatt aggattaaga atacttcaag gagtttttac gggttatgct actgcatgta
     6661 ctactcttat tgcaacacag acagataaaa atcattcagg atgggctttg ggtactcttg
     6721 caactgcatc aactactggc tcattaattg gtccaacagt aggaggatat atagaatcaa
     6781 tcttaggtct taaaagtaca tttattataa caggaggatt gttgtttgta tcatttatca
     6841 tctctatttt atttgtagta gataatttta aacctaaaga aataaaagaa tcaatttcta
     6901 tacctaaaga acaattaaat atattgccaa ataaattttt aattgcatct atttttgtaa
     6961 ccacattcat tactcaacta gctttatact ctatagaacc aattgtaacc atatatatat
     7021 cacaacttac aaattttgca tcaaatgtag ctttaatagc tggattgaca ttttctgcat
     7081 caggattagc aaatttatta gcagctcaaa aacttggaaa aatgtcggat aaaataggtc
     7141 ctcaaaaagt attgttaatt tcacttttat gggctgggat tatatttata ccacaagctt
     7201 ttgtaaaaac tgcatggcaa ctgatgttgt tgagattttt attagggttg tcagttgcag
     7261 ggcttaatcc ttctgttaac agcttgctta aaaaaattgc accagaagaa tatgtgggaa
     7321 aaatatttgg atataatgca tctgcccagt atataggctg ttcatcagga gcatttttag
     7381 ggggacaaat atcagcacat ttaggtatta gaacagtatt tttttcaaca agtttattgt
     7441 tgtttaggaa tgcactttgg atgtataaat ttactggatt gtttattaaa gataagacta
     7501 aataatcata ctatttacat ttctaatttt atgatgtaaa ataaatattt tttatgtaat
     7561 ttatcataga attatataaa aaataaccct atggtaaaaa ttggaaaata ttattatcga
     7621 caaaatcttt gacttaaaaa ctatattgcg atagtattaa tttaataaaa gtttgagagg
     7681 ggcgtaaggt tatgacaatt tcaaaaatac cacaagcaga attaaaagtt atgaagttta
     7741 tttgggaaaa aaatgataca gtaacatcaa aagaaactat tgaagctatg gaacgaaaat
     7801 atggttggaa acaaacaact acattgacac ttttatcaag acttgttaaa aaacattttt
     7861 taagtgcaga aaaaatagat agatacactc attatacaat agttgttgga cataaggagt
     7921 atttaagtgt tgagacaaaa gattttttaa gcaatataca cgacaattct ttacagagtt
     7981 taatttcagc actacatgat gatgaagttg ttgataaaga tatgttggat ttatttgaaa
     8041 gtcattttaa aaatttagaa gaagagtaat tatatcagga tagaactcta agaatttaat
     8101 taatctatgg ctgaaaacca taaatggtat tatttaatca gctactcact attcataaag
     8161 taatattaaa taggttctct aattagtaaa atgcctaaaa
//
LOCUS       DACULQ010000159         8142 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.160, whole genome shotgun sequence.
ACCESSION   DACULQ010000159 DACULQ010000000
VERSION     DACULQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.160"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(393..1373)
                     /locus_tag="JA154_14980"
     CDS             complement(393..1373)
                     /locus_tag="JA154_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997147.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(1537..2409)
                     /locus_tag="JA154_14985"
     CDS             complement(1537..2409)
                     /locus_tag="JA154_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAT7997148.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITFKYEK"
     gene            complement(2573..3124)
                     /locus_tag="JA154_14990"
     CDS             complement(2573..3124)
                     /locus_tag="JA154_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT7997149.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(3144..4046)
                     /locus_tag="JA154_14995"
     CDS             complement(3144..4046)
                     /locus_tag="JA154_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAT7997150.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDD
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(4061..6433)
                     /locus_tag="JA154_15000"
                     /pseudo
     CDS             complement(4061..6433)
                     /locus_tag="JA154_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
     gene            complement(6654..7343)
                     /locus_tag="JA154_15005"
     CDS             complement(6654..7343)
                     /locus_tag="JA154_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997151.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVYDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(7584..>8142)
                     /locus_tag="JA154_15010"
     CDS             complement(7584..>8142)
                     /locus_tag="JA154_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT7997152.1"
                     /translation="IYCIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIIN
                     EKSEICLKKGIKFTCSINFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENVNKYIR
                     IKGTMTRSFFVLKCENSKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELL
                     FENSIDKFILNIYIPLDQDTDSWGD"
BASE COUNT         2601 a         1454 c          780 g         3307 t
ORIGIN      
        1 ttaaataaaa aaaaaaccat aaattttaaa tttatggatt aattcttgta atgataactc
       61 tatttgagga tgtgagggag ggttatcatt acaatagggt attatattat ggtttttaag
      121 ctagtctttc tacacctcat atgatacata tgctatatga attttatatg atttttacat
      181 aaatatttta tgaaatttaa attttttagt cattttgaaa tttgtaaaaa gttctctaaa
      241 aatccacagc atcataaaca gccaaaattt aagtaaaaca tcataaaaaa atttgtccaa
      301 taattttaac aagtaatctc acctcattat aattattgac acaaacttaa ataaacaatc
      361 tataaatctt ctataaatct taaactttcc ccctattgat acacaatttc tagtttgtca
      421 attttatttt gcatttctaa gaaaatttct tcaacagaat tgttaaatat attgcctaaa
      481 tagattcttt tatctttttg aaatgaataa gattttacac taccatcaga agaaatatat
      541 aaatgactcc actctgctcc atattttgaa aacctctcct ttgtatcttt aaacctcata
      601 tcttctattt ttattgcttt ttctctgtct cctcttacta cagtgtgacc cctttttatt
      661 aaactggtta tttttatatc ttcttttaat ttaatacgtg tatcatcatt ttttgaatat
      721 actttccata tcactttatg acgataatta tctaattcct ttattaagct tctatcaata
      781 gtaaaactat ctttagttac cacaattaaa tcaaaatggt ctaacatata ttgaaatatt
      841 tctctcatac ttttattttt taaaatatcc tcaccaataa cctctataaa tctacagcct
      901 ttctcataca tgcagttcaa taaacttgtg atttcagaaa aataatcatt tgtattatta
      961 tctccatcta tttctatagt aaagtacaaa ggagtaatgt atgatttatt tcctgttatc
     1021 tcaaatacac ttgatagtgt ctgtatatca tcttcaaacg ttaagtacac attctcataa
     1081 aacataaaaa attccataac tttttctttc atattatgga tatttctata tctattggat
     1141 aatatattta ttatatcgtc agtagtattt ttcccatcac attcagccag tataacaatt
     1201 tgcttccaac ttaagctatc cacatttccg atactttttt tatatccatc ttcttcgtca
     1261 taaataggac caattataat gttttctttt ggccacagat gaaccctagt tccatctttt
     1321 aatttcggat atttattttt tacagactca taaaatctac ttgtatcacc catgattctt
     1381 tactaccccc catagtttaa atattctatg ctaaaaatag cactttattt ttattaattc
     1441 aacaattatt caaaattggt cattatatat gtttaaatgg atttaacatc aaccttattt
     1501 atatgttatt gatgtttata tatgttatat aaaatcttat ttctcatact taaatgtaat
     1561 cttaactatc tctggtttac aattaagcct aattaaaact tttgagcctc ctaagccttt
     1621 atttaaaatc atagtgctat tatctttttc atattttccc cagtcatact taggaaagaa
     1681 ctctctattt ggagataata cacctccaac aaaaggtatt cttattatac ctccatgaac
     1741 atgtcctgct aaaaccaaat ctgctcccca ttttgcatat ttttcaaaga aaaatggtgt
     1801 atgtgctaaa agaatgttat attcattttc attaacttta cctagattgt cttctacaaa
     1861 attgtcactt agttctaatt ttttgttatt agcaaataaa tatggatagc attccaatgg
     1921 tataattaaa ccatataaat taagatggct attacctcta tatattctta cagattcatt
     1981 ttccatattt attattttct ttgaatatag ttccttaaaa taatccttat atagatgttt
     2041 atactttttt attaaagatt tttgttcatg atttccaatt atatgatata cttcatattt
     2101 ttcaaccaaa ttatctatta aatctaatgc aacttgaaaa tttttatttt ctccatctac
     2161 taaatctcct gttataaata taacatctgg gtttaatgat tcaattttat ctatcaatac
     2221 cttattattt ttatcaaaca ccttattatg taaatcactc acttgaacta ttttaaagtt
     2281 atcaaattcc tttggaattt ttttgttgaa aatagtatat tctgtcgttt ctatatgctg
     2341 agtttcatac ttatacagaa tatacaataa tattaatact aatactgtca ttgaaacctt
     2401 tataatcata ttctcttcct ctttattcta ttttgttaat attattataa tgcttttatg
     2461 ttttttttaa aacaataaca tcatcacata catataaatc ttttcgacat tataaaaata
     2521 agttatcctt ttctaaaatt ttcatttatt ttagaaaagg ataactataa aattatctat
     2581 aaacctctat attgtgaaat atagtttttc ctttatttaa atttttatat atatgctcta
     2641 aagttccatc aaacctaata gaatgtccag actctaaaat ataagttttc ccatccacat
     2701 taaggtcaag tttaccttct attacgagca catattcttc taaaacatga ctatgtgtag
     2761 atgaaacatg agtacagttt tcctcaagct caattataaa aagttcaaac cttctttgag
     2821 ggctaaaagg gtatattgga tataacttca tgttcccttt ttgctcaaca ataggctcta
     2881 cttcttcttt ctttataacc tcataggtat ttgtattttc atttaatagt gtagtaaaat
     2941 ttattttaaa tccagatgaa attttccata aagtagatac tgtcggactt gattcacctc
     3001 tctcaatctg tccaagcatt gctttactga cacctgtcat ctttgaagct ttgtctaaac
     3061 ttatgcccat ttcttgtctc aataacctta aattttctcc cacattagga aatttattat
     3121 ccatgttaaa atcctcctga tagctatttg tattctatga tttttactcc tgtaagttct
     3181 actatctctt ttaattttaa catatgattg ttacaaatat catcaaattc atatatataa
     3241 tcttcaagcc ctaatgcctt atatttatcg attccaaatt tatgatatgg aagtaactcc
     3301 atcttgcctc ctggaacatt ttgtttgaca aataatgcag tatttcttat attttcttca
     3361 ctatcattta ctccataaat aattggcact cttataacca tagatttatt caactttgaa
     3421 agcctacata tattatctaa tataatttta ttacttactc ctgtatattc tttatgaata
     3481 ttatcatcca tagattttat atctacaaat atgtggtcta ttttctcaaa tacatcattt
     3541 accttattcc aatcaaaata tccacaagtt tcaattgcaa tatttatgcc tttatcataa
     3601 aaaatatcaa ctaattctct taaaaaatca atctgaagtg taggttcacc tccagaaaaa
     3661 gttatccctc cattagattc aaagaaaaat ataaaatctt tttcaacttc ctctactatt
     3721 tcttcaacac tcatttcttc ggtcattata caaatggcat ctttaagaca taccttgata
     3781 cactctccac ataaatcaca tttattattt atttgcgctc tatcaaatag tgaacttata
     3841 ttttgaggac aaacatctat acatctatta caggatacac atttatcttt cataacacct
     3901 aattttacta tgttactcca ggaatctgga ttagaacacc acttacattt taatttacat
     3961 ccttctaaaa atatagtagt ccttattcca tctccatcat ttactgaaaa atgttgtact
     4021 tttacaactc tacctttttt actcataatc tacctcataa ttaaaaattg ctatgtgaat
     4081 ttctagctat tatagcatct tgcatttctc ttgataaatt aacaaattga gtactatatc
     4141 cagcaaccct cactaataaa tctttatatt cttctggatt ttcttgagct tttaatagag
     4201 tatcatttga tatcgtatta aattgtacat ggaatcctcc taaagcaaaa tatgatttaa
     4261 tcatagatgt tagatttcta agtcctcttt cagtttccac caaatcttga ttcagtctta
     4321 aatttagaag tgttccacca gtatgaacat catgatttat ttttgatgca gatttcattg
     4381 ctgctagtgg acttgttgta tcagtaccaa caaatggaga aactccctct gtcaaaggtt
     4441 ttgtcgcttt tcttccacaa ggagttgcac ctactatttt tccagttggt acatagtttg
     4501 atatacccat aaatgcacta ttaaatttag agccaaaaat atccttgtat tttctagttt
     4561 cagtataata aaaatcagaa acttcaattg ctaatgaatc tacataatca ttatcatttc
     4621 catacttagg aacatccaac gcaagttttc ttaattcttc ataaccttcc caatcattat
     4681 ttagtgcttt tgttaattat tctaacgtag tcaccttatc ctcaaatact aattttttta
     4741 tagctgccaa tgaatttgaa actacaccta accctatacc agttaataca ggacctatat
     4801 tatattttgc tcctccttta cttaaatcaa ctcccttatc caaacatcca tctacacaag
     4861 tagataaaaa tggtcttggt accatttctt tatgaatttg ttgagcaaca gtagttccta
     4921 taacagaact ttttacaaga tatgctaatt gctttaaaaa ctcatcctta agttcttcaa
     4981 aagaagtaaa ttctgtgata ttttttgtat ctaaccccat tttttctcca gttaatctac
     5041 tcttaccttc attcatagca tattctaaag ctgctgcaaa attgatattt acggctgaag
     5101 tccactgtcc tgtttttcta aaatgaggaa ctacacaacc acagttactc caatctctag
     5161 catcttctgc atcatatcca tcttgtaata acatttgtgc ccctgcacta tcgctgtgta
     5221 tagcagggaa accagttcct tgttttacca atttagcaac tgccattacg aaattatctg
     5281 gtgctccttg actcactcta acacttaatc ctggttggtg agtttttaca ctttctgtag
     5341 cctttaaaca catatatgag atatcattcg ttccatcagt accatttctc tttttaccac
     5401 caacagtaag attttggaat tgattatatc ctgcaaagta atttgctgta tttgctgata
     5461 tagtccatac ccattctgat aacttaatcc aaagtgcttc tatcaattct tgagcttctg
     5521 attcagttat tttaccttct ctagcatcat tttcataata tggatacata tattggtcaa
     5581 atctacctaa atttaatgcc aatggatttt ctgatagaat accacctaat tgaacaaacc
     5641 atacaaattg tattgcttca taaaaattag ttggagggtt tgctggtaca ttcatacata
     5701 tctctgaaat ttttataagt tctaactttc tcttttcatc tgtttcttgc ttactcatct
     5761 cttttgcaag atttgaatat ctttgtgcca atgtcataat accctgtgca actatagaaa
     5821 cagatttgta aaaatctatc ttttcaatat tttctgaaac tgatatatct aattctttaa
     5881 tttttttatc tgcatcttcc ttaatccctt tatatccttt tacaaacaat atatcttgat
     5941 aatctggtgt tacttcacca actgcctgtc tccacttaga atcattatct attattcctg
     6001 tatctactaa tatcttagct gtatcttctg ggattcttgc taagaaaact tcttccaaag
     6061 attttccctt ccaatatgga aatatttctt ttcttataaa ttctatttgt tcttttgata
     6121 ttacatatgg gtcttgagtt cttttatcaa aagtatccat ttctttgtct acccattgcc
     6181 aagaaaactc tggtgtcaat atccctgttc ttctaaattt tcctgctgta cctactatta
     6241 attcattttc aaagattttt ataggaagtt ctttacatgt ctccaaaaaa gctttagctc
     6301 ttcttataca gatagcttct ccctcagttt ttttatgtga ttctgtaaaa attctagccc
     6361 tttcacaaca tatcataggt ttagaattaa tatattcttg tctaaatgct tctattctat
     6421 tagtttgact catttaaaat ccccctttgg ataatatata ctacattttt gtatgttata
     6481 acatacttta attatattat actgtccttt agcataaaat ggaagcaaaa taacttattt
     6541 tttttcatct accttgaaca cctatatatt aatacttttt ttgttaaatt tataaactct
     6601 aaaaaaattt tctatgatat attttctata ttgttatagt tatttctaat atactaatga
     6661 tttgaaatat caaatttgta tactagactt agttttaaaa ccttagttga ttgtttaact
     6721 ttgttaactt caacatgtat atccttcata taaggaagct ctacaacacc tactccctta
     6781 ttaaagtctt cttcaaccat agaaaaaatt tctttctcag ttaagttcgt attagtcaat
     6841 gacttaaata aattgtatat aaatttaata tatttatcac tctcagataa cttatcatct
     6901 tctggatact ctttagcaca gagaatatct attgttactt ttccttcttt atcgtcttca
     6961 gacatagaat agaataaact tcgaatattt ttttcaggtg gtttttcagc accatttata
     7021 gatactacaa agctaccatc tgattttgag tcagtaatac tataaccact gtctattata
     7081 gctttcgaaa atctttgaga taaatctttt tttacttctt ctctaccttt cgcatcatta
     7141 atcttttgtg agtaattatt aaaaagctcg atagattcat cttttttatc ttcaacttgt
     7201 tctttcaacg aatctttact aatttgttct gagctactta agttcttatc atatacagga
     7261 gacatacacc ctaccatcaa tgctaagcta agatttaaag ccacaatgat tgccactcta
     7321 tttatattcc tttttacttt catacacaat cctcccatca cactaaccgc tctaaagtat
     7381 tacttattgc actagaatat gtttatattt tctttatatt aaagatttat tattcaatac
     7441 atatcacctt cctatccaat tcttataact tactattagt aaaattataa gctccttaat
     7501 acagaaataa ggatgttatc ttttgttatt aaaagataac atccttattt ttttgtatca
     7561 atacatatgt cccaactggt aacttaatcg ccccaactgt cagtatcttg gtccaatggt
     7621 atatatatat ttagtataaa tttatcgata ctattttcaa ataataattc tccattatat
     7681 ttttgtaaag aagatttaat actctgaata cctattccat gtacaaactt atccattttg
     7741 tttgttaaca atatattttt attaaaagta agctgattta gtttactatt ttcacattta
     7801 agtacaaaaa atgaccttgt catagttcct ttaattctaa tatatttatt aacattttca
     7861 tccactattt tatcacatgc ttctattgca ttatccaaaa tattagaaaa tatgcttgac
     7921 acatctatag gttttacaaa atttactttt gaaaaattta tagagcaagt aaatttaatt
     7981 ccttttttta aacatatttc actcttttcg ttgattataa tgtctaatat catatttcct
     8041 gtattataga tatatttaaa tgtttttatt tcatctttta aattgttaac atattcatta
     8101 atttctttgc tattgtttct aaggttgtct atacaataaa tg
//
LOCUS       DACULQ010000160         8066 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.161, whole genome shotgun sequence.
ACCESSION   DACULQ010000160 DACULQ010000000
VERSION     DACULQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.161"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     misc_binding    101..311
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            537..1352
                     /locus_tag="JA154_15015"
     CDS             537..1352
                     /locus_tag="JA154_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT7997153.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            1380..2057
                     /locus_tag="JA154_15020"
     CDS             1380..2057
                     /locus_tag="JA154_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAT7997154.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            2116..2838
                     /locus_tag="JA154_15025"
     CDS             2116..2838
                     /locus_tag="JA154_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HAT7997155.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            3486..4637
                     /locus_tag="JA154_15030"
     CDS             3486..4637
                     /locus_tag="JA154_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HAT7997156.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRLSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            4683..5858
                     /locus_tag="JA154_15035"
     CDS             4683..5858
                     /locus_tag="JA154_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAT7997157.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSILPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            6014..6817
                     /locus_tag="JA154_15040"
     CDS             6014..6817
                     /locus_tag="JA154_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAT7997158.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFSQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYSPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            7060..7701
                     /locus_tag="JA154_15045"
     CDS             7060..7701
                     /locus_tag="JA154_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductase"
                     /protein_id="HAT7997159.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         3225 a          780 c         1532 g         2529 t
ORIGIN      
        1 tagtatttag ataatttaca agtaaaaatt attaaaaaaa taaaaaactt attgacaaaa
       61 atattataag gaagtacaat tgtattaaca atttaaggaa acaaatccaa tgatagagaa
      121 agtagcatat atggatttac agcgagttag gggttggtgt aagcctaaca atgaagtcta
      181 tgtgaagaga gctctggagg agattatcag aaactttagt atgataattg gtttacccgc
      241 cttaagggat tgagaggata agtacttact tatcaaaaag agtggtaacg cggatataat
      301 tcgtctctta gctgtaaagc taagggactt ttttgatttt aaaataaaaa tatccataca
      361 gcttaaaagc taacagtcac aaattttgaa agtaatataa gtatttatca ataagagtgg
      421 taacgcggta aagtcgtctc ttagtcatta aagactgaga gacttttttt gtaactcgaa
      481 acatttaagt attaaaatct gagggaaatt ataattaaaa gagaggggta gttaaaatga
      541 aagtatttaa aaaattatta agtttaggat tagtattagg attgacatta tcgctagtag
      601 gatgtagtgg tggaggagaa aaaacaaagt tagaacaaat aaaagacaat ggaaaattag
      661 ttgttggtac tagtgcagaa tttcctccat ttgaatttca taaggtagta gatggaaaag
      721 attcaataaa aggattcgat ataatgttgg ctgaagaatt tgcaaaagag cttggtgtaa
      781 aggttgaaat taaggatatg tcatttgatg gattaatagg tgctttaaat gcagaccaag
      841 ttgatattgt tcttgcaggt atgtctccaa caccagagag agaaaagagt gttgattttt
      901 cagaattata ttatttaagt agaaatgcag ttatagttaa agatgcagat atagacaaag
      961 taaaaactga agatgattta aagaaactta gagttggagt tcaagcagga agtatccaag
     1021 aagaatatgt agttaacact ttaaaaatga caactacaaa atctttgaaa gcaatacctg
     1081 atttaattac agaattaaaa aatggaaaca tagatgcagt tgttactaat gaagcagttt
     1141 ctttaataaa tgttaaaaaa tatgatggaa taaaaatggc aaatactgaa gttgggaaag
     1201 atgtaacaga aggtatggca gcagcaatca aaaaatctga caataacaaa gattttatag
     1261 aattattaaa caaaaagata aaagaattac aagatggcaa gaagatagaa gaatttttaa
     1321 atgaagcatc tactgaagct gcttcaaatt agtaagaaag ttatgtggga gatgaaatta
     1381 tgagtttaga ttttagtttt ttaagtagat ttgggacttc ttttttggaa ggaacaggtg
     1441 tgacagtatc aatttctctt gtggcattat gctttggatt tataataggt ataattatct
     1501 gtatggcaaa aatatcaaag agcaaagtat taagagcaat atcatcaatc tatattgagg
     1561 ttttaagagg gacaccatta ttagtacaga tatatatagt atggtttggt ttacctcaat
     1621 taggaataag attccctatg ttgtttggta taccaagtga atttatagct agtgcatttg
     1681 cattatcagt aaactctggt gcatacgtgg cagagatact tagatcaggt atacaatcag
     1741 ttgataatgg acaaatggaa gcaagtagat cattaggatt aaattattgg tcaactatga
     1801 gatatataat aatacctcaa gctataaaaa atatattacc ttcattagca aatgagttta
     1861 ttacacttgt aaaagaatct tcaataatat ctgtaatagg agttgttgag ataatgcgta
     1921 ctgctgatat agtaaaaaat gcagcattta gagcattaga gccattaata gtagcagcag
     1981 caatttactt tgttataaca tttactctat caagattagt tggattatta gaaaagaaac
     2041 tatctgtgtc taactagatg agagcatgtt tagcatttag ggaaaacata gaatagtaaa
     2101 aaggaaagtg ataatatgat tacaataaaa aatttaagta aatcttttgg agatttaaat
     2161 gtattaaaaa atatagactt agaaatcgct aaaggcgaaa taatggttat agtaggtcca
     2221 agtggttctg gtaaaagtac acttcttaga tgtatgaatt tactagaaat acctacaggt
     2281 ggagaaataa tatttgaagg aaaaaatttg gtcgataaaa aaactaatat tgatgaggtt
     2341 agacaaaata taggtatggt gtttcagaac tttaatttat tcccacataa gactatttta
     2401 gataatataa cattagcacc aattaaatta aaaaagatga ctaaggaaga agcagaaaaa
     2461 aaagcagaaa ttttgttgag tagagttggt cttttagata aaaaagattc atatccatca
     2521 caattatctg gaggacaaaa gcaaagaata gcaatagcta gagcacttgc tatggaacca
     2581 gatatgatgt tgtttgatga gccaacatct gctcttgacc cagaaatggt aaacgaagtt
     2641 ttggatgtta taaaagaact tgctaaagaa ggtatgacaa tggcaattgt aactcatgag
     2701 atgggatttg ctaaggaagt tgcagataga gttattttta tagatggagg aagtatactt
     2761 gaagataata ctccagaaga agttttcgga aatccaaaac atgaaagaac aaaagcgttt
     2821 ttagcaaaag tgctataata tataaaaagg atgttattga tattagaaat tcaatacatc
     2881 cttttttagt gatggttaat attaatttta aaattaaagt tatattaata ttaactttat
     2941 ttttacgatt ttttgcagaa aaaaaaagag ctcggggtaa gctctttttt tccaccgaat
     3001 taatttccgt tactaatccg atttttaata aatataaaat atatccttat gaaagggtat
     3061 tgggaataga tatattaact aactagggtt accaggggga taaccttaat ttattataac
     3121 aaaatgcgac acaaaaatca atattttttt tgaaataaat tataaaagtt atttttataa
     3181 caagtaagct tataaattta acttttatag taaaaataag tatatgtata atataatata
     3241 tttattttat ataatatatt attttatcat atattgtatt ttataatata tttttgtagg
     3301 gtatagtata agtatattat attttactat aaaaatttgt aataaagtga aaaaactagc
     3361 ttttcatgaa ataatatttt ataatttaag attgtctttt taataaagat taacaaaaaa
     3421 aatactataa ttagtggtat aatagttaga gttgaagatt ttaaataaag gtaggtgtaa
     3481 ttaagttgga aatatactta gacaatagcg caactacaaa accttatcaa gaagttatag
     3541 ataaaatggt ttatgcactt aatacggaat atggaaatcc ttcttctgtc catagaaaag
     3601 gtgtagaagt tgaaaaagct ataaaagaag taagacaaga tatagctaaa tctctaggag
     3661 cgaaagaaaa ggagatttat tttacatcag gtggtacaga gtgtaataat actataataa
     3721 gaggtattac tagtttaaat aaaaaaagaa aaaatcacat aatatctact aacatagaac
     3781 atccttctgt tttaaataca ttaaaagatt tagaagaaga tggttttgaa gtaacttatt
     3841 tagaggtcgg aaaagatggg aaaataaata tagaggattt aaaaaatgcg ataaagtcaa
     3901 ctacatgctt agtaagcatg atgcatgtta acaatgaaat aggaactata cagccaatag
     3961 gtgaagttgg aaagtatttg aagggtttaa aagaaaaaat ttatttccat gttgatgcta
     4021 ttcagtcata tggaaaaatt aattttagac tatctaaata caatatagat tttatgagtg
     4081 taagtgcaca taaatttcat ggtccaaagg gaattggatt tatgtatata aaagaaaata
     4141 atcgccttaa gcctatgtta acaggtggtg gtcaggaaat tggaattagg tcgggaacag
     4201 aaaatgtacc tggaatatat ggtcttggag aagctgttag aatactaaac aaagatttag
     4261 atgttgttat ttcgaaagta aataacttaa aaaatatatt aaaaaatgaa ataattgata
     4321 atatagaaga tataaaaatt aattctccag aagatggagt atgtcatata ttaaatgtat
     4381 catttagagg gacaaaagga gaagttttac ttcattactt agagcaaaag ggtatttatg
     4441 tatcaactgg ctctgcatgt tcttctaaaa agaaaggtag ttacgtatta aatgctatag
     4501 gtcttacaaa tgaagaaata aatggaacaa taagatttag tttatctgat atgaatacag
     4561 aagaagaaat gttagaagct gtgaaagttt tgaaagaatc aatttgtgac ttaaggtcaa
     4621 taatgaaaag aaaataaaga atactgtata gatagtgata ataaattagt ttaagaggtg
     4681 aaatggtgta taatatatta atagtaaagt atggagaaat tggagtaaaa ggaaagaata
     4741 gatatatatt tgagaatagg cttataagaa acataagaaa tatgttaaaa ccaattggaa
     4801 gatttaatgt atataaagag tatggaagaa tatatgttga tttagaagac tatgattatg
     4861 aagaagtaat tgaagaagtt agaaaagtat ttgggatagt gggtgtgtgc cctggagtaa
     4921 gagctaaaaa agattatgat acattaaaag aaatagcttt aaaaatgtta gaagaaaaaa
     4981 tagaggctgg atataaaact tttaaggtag agtcaagaag aggagataag tctttcgggt
     5041 tgacttctca agaaatgagt atggatatag gtggatactt attatcaaag gtaggagata
     5101 ggattaatgt tgatgtaaga aatccagaag ttaaaataaa gtgcgaatac agagaatttc
     5161 atactatggt ctatagtgat actatacctg gatatggtgg attgccactt ggaacgaatg
     5221 gtagagctat gtctctttta tcaggaggta tagatagccc agtagctaca tggatggttg
     5281 caaaaagagg tatggaagta gaggcagttc actttcatag ttatccattt acaagtgaaa
     5341 ggtcacagga aaaagttaag gatttagcta aaatactggc aaaatactgt ggtagagtaa
     5401 ggcttcataa agttaatatc ttagaaatac aaaaagcaat tggcgagaat tgtaatgaag
     5461 aagaagctac tattctttca agaagattta tgatgaggat agcacagaga ctttctgaaa
     5521 aaagacattg tgatgcttta attacaggtg aaagtattgg gcaggttgca tctcaaacta
     5581 tacaaggact tacttgtact aatgccgttg tagacttacc agtgtttaga ccacttatag
     5641 caatggataa atcagatata gttgatatag ccaaaaaaat agggactttt gaaacatcta
     5701 tattgcctga agaagattgt tgtagtgtat tttctcctag aaagcccgtc actaaaccta
     5761 gattagagaa aatagaaaaa tctgaaacag cacttgacat tgaaaaatta gtacaggatg
     5821 ctattgacaa aatagaggta gaggatattg agttttagca tacaagttat ttaattacta
     5881 aattgaatat tgagtatatt agtagttctg taagtatatt agtagttctg ttgaaaggat
     5941 ttatgtagac aatttattaa tatgtagtat ttaaaaataa gtactataat tttatttaat
     6001 gggaatgatt attatgaata aaaagggttt tacactaatt gaattgttag tagttatatc
     6061 tataataggc attttagtta tagtagctgt tccagcattg tttagaaata tagaaaaaag
     6121 taaagcagtt acatgtcttt ctaatagaga aaatataaag actcaaattg ttattgcaat
     6181 ggctgaggaa tcaagtaaag gcaagaatga agtcatgaaa gaggtattag aaaacaaaga
     6241 tggtaagtac tttgaaacag aaccaaagtg taagtcaggt ggaatatatt cagcaacgtt
     6301 tgatgatggt tatgatggaa taactggaat agaaagcatt gcaaaagtgt atgttacttg
     6361 tacaaaacat ccagatggtg ttgaaatggc tagggatata catcaaagta tgaaagattt
     6421 gattgcatca ttttcacaag acccttctat aataccagga gcctcaaagg gtaatgatga
     6481 ttttagaaaa tatttattag acaataaata taaaaatggg tggcctacaa ttccagatga
     6541 atttaaggca aaatatggat taagtaagga tacactatat atacaaccat atgcatatag
     6601 tcctactaaa tctgatgcta ctgtagttgt atttgcaaat aataagactg ggggtaattg
     6661 gtatacttcc ctagtttacg attatgatga aggtagatgg tataaaggta aaaatggtat
     6721 ttctgttgca ggtaggtcat gggatgttga cacagatagt gttaagtctg taaaaacaga
     6781 gattcattct aaagagggat ggggtccttt aaattaatat atcatgtttt attaatctgg
     6841 tattgattaa actatagaaa agagcagttt ctatagatga cgaataccag attttcattt
     6901 atatatataa ttgagatata ttaaaagtgt ttatattttt aagtcttcac atacaagcta
     6961 aaaataatta taataatatt gtaaagaaat ttaattataa gattaatttt ctgaagattt
     7021 agtatttgta ctaagaaata cataaaaagg ggaatagata tgaataataa ttttcaagac
     7081 aaccaaacaa taaatttaat acaaagtcgt agaagtatac gaaaatttac gacagaacaa
     7141 attagtgatg agcaagtaaa cacattatta cactgtgcat ttgcagcacc ttcaggatgt
     7201 aataaacagc cttggcacat aactgttgtg caagaccaga aacttttaaa agagattagt
     7261 gatgatactt tatctaggat tcatgaggtg agtaatgttg agataaataa aaactttaag
     7321 ttattttatg gtgctccaac cgttttattt atttcttatg atgaaagtag ttcatgggct
     7381 ccatatgata taggaatact tacagggaat ataacaacag ctgcacaagc actagggctt
     7441 ggaagttgta taataggaat ggttagaggt ttatttacac ctgttgaaca aggggatatt
     7501 gaaggtcttg tgagtgtatt ggataaagaa gatgtaaaag aatcagagtc aataaagatg
     7561 aagtttgata caaataaaaa atacagagaa ctacttgata taccagaagg gtattctgta
     7621 ccatttggta ttgctgtagg tattccagat ggaaatttac caaatgctag agaggtagtt
     7681 tataaagttt ccagagttta ggataaagta ttgcaagaac caatcagtta cttgaagaat
     7741 taaaccagta atatacaaat aaattgatgg aagaggaagc tatttaaaaa gtagtttaga
     7801 tttttcgttg taaaataatt aaataaagaa taaggtgata agtattttat ttattatctt
     7861 attctttttt atttttgttt tgtagaatga taaaaataag gattaagtgt aataaaatag
     7921 aaaacaagta tgttaatttt aagtatatgc tttaaaattt atccaagtta aaaatttaag
     7981 atatcttttc agtataatgg aaaaaatgtc aaaatattga aatagggaaa aaatagtgat
     8041 ataataagaa aaaatagaga aaaaaa
//
LOCUS       DACULQ010000161         7991 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.162, whole genome shotgun sequence.
ACCESSION   DACULQ010000161 DACULQ010000000
VERSION     DACULQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.162"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            139..1425
                     /locus_tag="JA154_15050"
     CDS             139..1425
                     /locus_tag="JA154_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAT7997160.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            1511..2818
                     /locus_tag="JA154_15055"
     CDS             1511..2818
                     /locus_tag="JA154_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2558..2560,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HAT7997161.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            3120..3827
                     /locus_tag="JA154_15060"
     CDS             3120..3827
                     /locus_tag="JA154_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7997162.1"
                     /translation="MISIAICDDESYMLNHLKENIELYMKQKNLLYNIELFNKVEDLL
                     CNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAFEVDAINYL
                     IKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVIKRKIYVHL
                     KKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGDRIPVSRLR
                     QQIFSKVMLEFMKNRRI"
     gene            3829..5187
                     /locus_tag="JA154_15065"
     CDS             3829..5187
                     /locus_tag="JA154_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT7997163.1"
                     /translation="MINLFSSDLFLKYSFLFSFIVYRILMVIVQYYFLISFTNVNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            5277..5711
                     /locus_tag="JA154_15070"
     CDS             5277..5711
                     /locus_tag="JA154_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997164.1"
                     /translation="MKVGNINGQAPITNGLDSCQSEVQGLEQKKKSLQDEIEKVRSDK
                     SIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEENNVSEVNNNLLERKKEPS
                     NNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRPI"
     gene            complement(5974..6210)
                     /locus_tag="JA154_15075"
     CDS             complement(5974..6210)
                     /locus_tag="JA154_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997165.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(6365..6589)
                     /locus_tag="JA154_15080"
     CDS             complement(6365..6589)
                     /locus_tag="JA154_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAT7997166.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(6686..7939)
                     /locus_tag="JA154_15085"
     CDS             complement(6686..7939)
                     /locus_tag="JA154_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HAT7997167.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
BASE COUNT         3122 a          918 c         1277 g         2674 t
ORIGIN      
        1 aaattaaaaa tgaaaatttc tatgacatta atacatagaa tatatatttt aaataattgg
       61 catagaaaat gctttatata ttaataaacg taataaatac ttttaattgc gattaaatat
      121 attaaggggg attttcatat gaagctagag ctagggaata tatttataaa aaatgttgaa
      181 tttggagaaa agacagaagt aaaagatgga gtactatata ttagtagcga agaaatagaa
      241 aagatagtat tagaagatga aaggattgta agtgtaaatg ttgaacttgc aagacctggc
      301 gaatcaataa gaatagctcc agttaaagat gtaatagagc ctagagttaa aattggagat
      361 gaaagcaaaa tatttcctgg aattataaat aaagtgaaga ctgtaggaag tggaagaacc
      421 catgtacttt taggagcttg tgttgtaact tgtggaaata tagttggatt tcaagaaggc
      481 gtaattgata tgagtggtcc tactgctaaa tatacacctt tttcaaaaac aaataatata
      541 tgtattgtta taaaagcaaa ggatggtata gatactcatg attatgaaga agcagctaga
      601 attgcaggat taaaaatagg tgcatatgta ggtgaggcag gaagagaagt tgagccagat
      661 gaagtcattg tatatgagac aaagccatta ttaaaacaag taaaagaata tccagacctg
      721 ccaacagtag catatgttca catgttacaa tcacaaggac ttttacatga cacttattac
      781 tatggggtgg atgctaaaca gatagtgcct acatttatgt atccaactga aataatggat
      841 ggagctataa tatctggaaa ctgtgtagca ccatgtgata aggttacaac attccatcat
      901 ttaaataatc cagttataca tgacttatat aaaagacatg gaaaagattt aaactttata
      961 ggagttattt tgactaatga gaacgtattc ttagtcgata aagagagaag ctctgatatg
     1021 gtagctaaat taatagaatt tttaggagtt gatggagttc tagtgacaga agaaggttat
     1081 gggaatccag atactgacct aatgatgaat tgtagaaaat gtagtgaagt tggagcaaat
     1141 gtagttttga taacggatga atttcctgga aaagatggca agagtcaatc tatagcagac
     1201 gctactaagg aagcagatgc agttgtttct tgtggtcaag gaaatctagt ggttcatttc
     1261 ccagcaatgg aaaaaattat aggaacttta gattatgttg aaatgatgat aggtgggtat
     1321 aaaggatgct taaatgaaga cggaagcatg gatgcagagc tacaaattat aatagcatca
     1381 actatagcaa atggctataa tcatttgaca gcaagatatt attaaataag tatttgaata
     1441 taaagttctg ttgatataat cattggtcta attgatgtaa caactataaa gtgatttgga
     1501 ggattaagag atgagtaaaa tcagagtggt tcattatata aacaatttct ttgctggtat
     1561 aggtggagag gaaaaagcag atattccgcc agaaaaaagg gctggtgtag taggacctgg
     1621 aatagcattt cagagtcaat ttaatgatga tgcagagata atctcaactg taatttgcgg
     1681 agatacgtat tttggtgaaa atattgagag tgcaactcaa gaacttttaa aaatgataaa
     1741 agaagaaagc cctgatttat ttattgcagg cccagcattt aatgcaggta gatatggagt
     1801 agcttgtggg actatttgta aggcagttga agaagagtta aatattcctg taattacagg
     1861 catgtataaa gaaaatccag gtgtagatat gtttaaatta gatttgcata tagtgtctac
     1921 aggaaattca gcagcaagtc ttagaaaggt agttccaata atgacaaaac taggtcttaa
     1981 attggttaga ggtgaggaaa taggacctcc tgaagaggaa ggatatataa tgagaggaat
     2041 aagaaaaaat ttcttccatg aacttagagg gtctgaaaga gcaattgata tgttggttaa
     2101 aaagatgaaa ggggaaagct ttgaaactga gtatccaatg cctgaatttg atagagttac
     2161 acctatgaaa cctgttaaag atttatctaa gataaaactt gccctagtaa cttctggagg
     2221 gatagttcca attgataatc cagacaaaat tgaatcttca agtgctacta aatatggaat
     2281 ttacgattta acagatatgg attctatgtc aaataaagaa tttacaacta tacatggggg
     2341 gtatgacaga gcatatgtcc ttgaaaatcc aaatttagtt gtaccactag atgtagttag
     2401 agagcttgaa aaagaaggtg taattggaga attagtagat tattttataa ctactacagg
     2461 aacaggaacg agtgttggca attctaagag atttggagaa gagttttcta aaaaattgtt
     2521 agaagatgat gtagatgctg taattttaac ttctacatga ggtacttgta ctcgttgtgg
     2581 agcaacgatg gtaaaagaga ttgagagagc tggtatagca gttgttcata tatgtacagt
     2641 agtaccaata tcacttacaa taggagctaa tagaattgtt ccagcagtag ggataccata
     2701 tccacttgga aatcctatgt taggagaaga ggaaagtaaa aaaattagaa agaatatagt
     2761 tttaaaagct ttaaatgcac taacagaaga tatagaagaa caaacagtat ttgaataata
     2821 aaaatacaac acaacactga aataaaaaaa gatatcaaaa aataattttt taaaattatt
     2881 ttacgatatc ttttttttat taggtaaatt cgaaatttgc attttttaaa attaaattta
     2941 taaaagtgat aaattagttt attataatat tgatacacaa attcaaatta gatatactat
     3001 gaaaagcgag attattacat tttggaacaa atattcaaca tattagatca tattaattga
     3061 acagaaaaaa ttaactttat ataataatta gaataatgat aagaaagaag gtgtatatat
     3121 tgatttcaat tgccatttgt gatgatgaat cctatatgct aaatcattta aaggaaaata
     3181 tagaattata catgaagcag aagaatttat tatataatat agagcttttt aataaagtag
     3241 aagatttact atgcaatcat gaaaagttta atattgtatt tttagatata cagatggaag
     3301 agattaacgg aatgcaggca gctagaaaat taaggtcata tggtaatgag tgctttataa
     3361 ttttcgttac agttttaaat gaatttgtat ttgatgcttt tgaagtagat gctataaatt
     3421 atctgataaa acctctatct aatgagaaac tatataaaac tttggataaa atagttacaa
     3481 aacttgatga taatgaaagt aattatatca caatacaaaa aggttattca tattggaaat
     3541 taaaacttgc tgatatttta tattgtgaag ttataaaaag aaagatttat gttcatttaa
     3601 agaaagagac tgtagattat tatgagacta ttgaaaattt agagagaagt ttaccaacaa
     3661 atttttttag gtgtcatcgt agttatataa tcaatttaca atatgtatat ggatatgaaa
     3721 atggtaatgc agttatggaa agtggagata gaatacctgt ttctagacta cgtcaacaaa
     3781 tattttcaaa agttatgctt gagttcatga aaaataggag gatataagat gattaattta
     3841 ttttcttccg atttattttt aaagtacagt tttttatttt cttttattgt atatagaatt
     3901 ttaatggtaa tagtgcaata ctattttcta atcagtttta caaatgttaa ttcaaaattt
     3961 tggtgtttta ttttatattc gataatttca atttttttaa tcattataaa tacatatttt
     4021 aatttatata atttattttc ttcacagatt ctattttttc ttatgacttt tttatttgta
     4081 aagtacataa taaaacaatc tactgtttta tctatagtat caactttgtt agtatattta
     4141 attgaacaat tagtatatgg atttacagct ccattaaatt acttgatatt tactagtgat
     4201 ataaatacag atatttataa atttgatata aatatgggac tatcttcttt aattactatt
     4261 ataatagctg gatttatata ttggtatagt tctaaaaagt ttaatattaa ggtcatgaac
     4321 tttgataaat atatagcaat tttgatgatt ccattgttgc ttataatatt atttatgcaa
     4381 tcatttgaat atagttcaaa tataaatata gatacaagtc ttgggattgt aaagctgcta
     4441 ttaaatactt ctataactga agaaattcaa gcttttcttt tttctataat tggaactata
     4501 tgtgtatttt tttctctatt tacctttaag aaactaattc aagctctcga agatgacaag
     4561 gagagggcaa ttatgaatca acagattcat gctcaaaaaa actatattga agaagcgaaa
     4621 tcacgattat cacaaactat atcctttaga catgatttta ataatcattt agctattgtc
     4681 aatggattgt tgaaaaaaga ccaaatttta aaagcacaag attatttaaa taagcttgaa
     4741 aaagtaacaa gtgacctatc attttcttgt aatacacaga atattgttat tgatgctctt
     4801 ttcaacaata aattaagtat agcaaaacaa ttaggcatag aggtagattg tgaagtaatt
     4861 ataccccaaa gcactaacat gaacgatttt gacctatgta tagtgtttgc taatgctatt
     4921 gacaatgcaa ttaaagcttg tagagttgta gataaacaaa ataggtattt aaagctttcc
     4981 actaagttag agggtggatt ttttatgata gaaataaaaa atagctataa tccaataaat
     5041 aattatacaa gtggctcagg tataggtctt ttaaatataa aagaaattgc taaaaaatat
     5101 caaggggcta taagtataga taagacatca gattattttc aaataaatgt attgctggtt
     5161 atttcattac attgcgctag tatttgataa caatttcatt gaaaatagta tttgtaagct
     5221 atattatgta cttgaataga gaaagtttga tatatagaaa tgaggaggtt actaaaatga
     5281 aagttggaaa tataaatggt caagcaccaa ttacaaatgg tttagatagt tgtcaaagtg
     5341 aggtacaagg cttagaacaa aagaaaaaat cattacagga tgaaatagag aaagtaagaa
     5401 gtgataaaag tattgatggt gaaacagcca aattaaaaat agaagcttta aaaaaagaaa
     5461 tggaagagat tgaaaataaa attcaagagt taaaaggaaa taaagcaaaa aaaacaaata
     5521 aagaagaaaa taatgtttca gaagtaaata ataatctatt agagagaaaa aaagaacctt
     5581 ctaataatga aaatgtaaaa caattatatg atgtttttga aagaagcaat gtagacgaac
     5641 aatgggataa tacttatcgt gttctccaag atgataaaaa cttaaaagta aaatttaata
     5701 gacctatata gtttatttat aaaaatagtt aatattttaa ttatcaccac tttttagaag
     5761 aatacacact ttattttaaa gacccacata tttatttaca gttagcacat ttatatgtgg
     5821 ttttataact attatttaca agtctatatt gataactttt acatagtata taatgtttaa
     5881 atacttattc aaatttatct atacataaac aaaaaaagag attttaaaat ctctttttag
     5941 attgttgact tatggctttt tgatttaata aaatcaagaa gccgtttgaa ttttatctgt
     6001 tggctgatac ccttctgata ttgaaacacc atttttgttt ccaaaatcgt ttaaaattaa
     6061 gaacttatta tgaatgacta tttcattatg aacaataagc agataacatg aatcttcaat
     6121 caatgtttta acagtagcat taaatgcttg cttatacagt tctatcgttt gtttgaactg
     6181 tccattaaag taaatatgag tcaataacat aactatatct acacaaatta atataaattt
     6241 tttatactgt acattatcta actgttgatt atctgtatta caacatgatt gaatttaaaa
     6301 agccatcaac acatagaatg ttgatggcct gaaaatagat ttaaaatctc ttttttatta
     6361 tatattaatt attttttaaa tttatatttt ttgcatcaga ttttctaata gctaagtcaa
     6421 accctctaaa tctaatcact atagggtcat taagaggagc tatctttttc acttcaactt
     6481 cagtgccatc tattagtcct aaggctagaa gtctcttcat aagtttttca tttccagcta
     6541 tattgtctat aattcccttt tgtccaagtt ttaagttgta aacagtcatt attaacacct
     6601 cctaatgaaa atcattatca aactttatat ttataatata taacaaaaag agcaaattgt
     6661 caatatataa caaaaaataa acatactaag ttagtatgtt tataaaaaat agtatacatg
     6721 ggatactaat agcatctagc aaaaaaccac ttactaatgg tacaactaaa aaggcttttg
     6781 gagaatcacc atatttttgt gtcaaagaac tcatatttgc cagagcgttc ggagtagctc
     6841 ctattccatg tccaatcaaa ccacttatta tgatggcaga atcaaaatct tttccaagta
     6901 ctctaaagca aataaatatt gaaaatagga ttataaagat tacttgaaaa gttactatta
     6961 agagaatagg aggtaataat gtagataatt caaataaatc tatattcata agtgataaag
     7021 ttaaaaaaat tcccaatgaa agttctttta aaaaatctat aagtttaaaa tttaattcta
     7081 gtatatttat cttatcattt aaatttctaa aaataacagc cgaaaacata caagttacaa
     7141 cagagggtaa tagtatatta aacgttatat agaagcatct acttataatt tctcctgtat
     7201 ttatacaaat aaatatgaga agaacctgtt ccaaaaatac agatggtgtt attgtttcgg
     7261 agaagttatt attttttatc ctattcttat ttctaaaaaa tttacttcca aatctcttta
     7321 gatttctatt atatctagga atcgacaagt ctatatttga tgatggtttt aatttatatt
     7381 tatttattag aaatttagct acaggacatc ccaatatacc acccataata agtcctattg
     7441 ttgcagcaga taatccaacc ccaactgctc cttctatacc gagattttct attgtaagtc
     7501 caaatgcagc agaataacca tgaccacctt ccatagatac atttccacac ataagcccta
     7561 aaagaggttc aagttttata atctttgata ttgtaatagc taatatattt tgacaaaatg
     7621 ccaatactcc acacaaaatc caatatctaa ataatacact accacctttc tttataagat
     7681 ttatgcttat ttcaagtcct atagttgtaa aaaagaaggt tatgaaattt ggcattaaat
     7741 ttccacttat agatatatct gctatattaa aatatttaaa aaataaattt agaaaacaaa
     7801 ataatagacc tcctattact ggtgcaggta tacagaaatt atttaaaaag ttaactttat
     7861 tcttcaataa attaccaatt aaaaagaaga atatagatag tgtcaaggtt tgcactatat
     7921 caaaacttaa ggtaatcaaa ataagtcctc ctttgtaatt caaagtttta attatataac
     7981 ttaaattctt a
//
LOCUS       DACULQ010000162         7885 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.163, whole genome shotgun sequence.
ACCESSION   DACULQ010000162 DACULQ010000000
VERSION     DACULQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.163"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            27..101
                     /locus_tag="JA154_15090"
     tRNA            27..101
                     /locus_tag="JA154_15090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="JA154_15095"
     tRNA            116..200
                     /locus_tag="JA154_15095"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="JA154_15100"
     tRNA            210..293
                     /locus_tag="JA154_15100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="JA154_15105"
     tRNA            317..391
                     /locus_tag="JA154_15105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="JA154_15110"
     tRNA            416..492
                     /locus_tag="JA154_15110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="JA154_15115"
     tRNA            502..577
                     /locus_tag="JA154_15115"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="JA154_15120"
     tRNA            586..661
                     /locus_tag="JA154_15120"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="JA154_15125"
     tRNA            664..752
                     /locus_tag="JA154_15125"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="JA154_15130"
     tRNA            756..831
                     /locus_tag="JA154_15130"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="JA154_15135"
     tRNA            838..911
                     /locus_tag="JA154_15135"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="JA154_15140"
     tRNA            926..1002
                     /locus_tag="JA154_15140"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="JA154_15145"
     tRNA            1020..1096
                     /locus_tag="JA154_15145"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="JA154_15150"
     tRNA            1105..1180
                     /locus_tag="JA154_15150"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="JA154_15155"
     tRNA            1188..1261
                     /locus_tag="JA154_15155"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="JA154_15160"
     tRNA            1269..1345
                     /locus_tag="JA154_15160"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="JA154_15165"
     tRNA            1358..1433
                     /locus_tag="JA154_15165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="JA154_15170"
     CDS             1509..1922
                     /locus_tag="JA154_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997168.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="JA154_15175"
     CDS             2231..5662
                     /locus_tag="JA154_15175"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086484.1"
                     /note="biotin-containing enzyme that catalyzes a two step
                     carboxylation of pyruvate to oxaloacetate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAT7997169.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /gene="fusA"
                     /locus_tag="JA154_15180"
     CDS             5904..7832
                     /gene="fusA"
                     /locus_tag="JA154_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086485.2"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAT7997170.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3091 a         1010 c         1584 g         2200 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctgta aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacactag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag ataatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacagaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7381 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttag
//
LOCUS       DACULQ010000163         7865 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.164, whole genome shotgun sequence.
ACCESSION   DACULQ010000163 DACULQ010000000
VERSION     DACULQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.164"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1760)
                     /locus_tag="JA154_15185"
     CDS             complement(<1..1760)
                     /locus_tag="JA154_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997171.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDT"
     misc_feature    1855..1911
                     /note="possible 23S ribosomal RNA but 16S or 23S rRNA
                     prediction is too short"
     gene            complement(1943..3418)
                     /locus_tag="JA154_15190"
     CDS             complement(1943..3418)
                     /locus_tag="JA154_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HAT7997172.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3590..4885)
                     /locus_tag="JA154_15195"
     CDS             complement(3590..4885)
                     /locus_tag="JA154_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7997173.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(5069..6514)
                     /locus_tag="JA154_15200"
     CDS             complement(5069..6514)
                     /locus_tag="JA154_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT7997174.1"
                     /translation="MSLNKFEIGGITVKKKIGFWDLVFMNISALFGIRWIAKSTASSF
                     GLGLGSIPVWLLFAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLN
                     WTSKIFWYSSFLTFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGK
                     FFTNTGALGSTIPAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAII
                     FALSGAETTANFITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAAST
                     GILDALAKVAQDLGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQ
                     LTVTNKYNIPSNAVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFT
                     SYIRLRKTRPNEERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYE
                     IEMIGGGLFVIITGLMLWKNYEKKISNNSGR"
     gene            complement(6530..7669)
                     /locus_tag="JA154_15205"
     CDS             complement(6530..7669)
                     /locus_tag="JA154_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAT7997175.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVEGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
BASE COUNT         2481 a         1462 c          858 g         3064 t
ORIGIN      
        1 tttgtatctt gatttttgtt agaaatactt tccctataag gaaaagaatg ttcattggta
       61 ccaaatactg attttgagct taaatcataa ttatcatcat tttctgtaat tgaattttta
      121 ttgtaaaatc ctttttcttt atcagaatta ttttctttat taccgtcttt aggtatatca
      181 tgaggatttt tattttcatt gctatcagta cttgattcta catcatcttt aggagtattt
      241 ggttgtttct catcagaatt atcattttca ttattattat tttcattatt attgttttga
      301 ttgttattac tatcattatt ttcatttcca ttattcccat cttgattgtt atcatcctca
      361 tctctatccg ggtcgtctct acctcctcct ccacggtcac catcaatatc atcattttcc
      421 cgttcatcat ctggcgtctc ttgattgtct ccgcctccca ttataaatcc tggtttaaaa
      481 actatatttg aatacccttc ttttaatcct cctaccactt ttactctaaa acaacgcaac
      541 ttaaaatctt caaaaaaaag tatagcttga tctctaatat cctcctgttc ttcagatatc
      601 caattgattc catcgcttga atattcaaca aaaaccttac ttgcactata cggcatatca
      661 taaaataaaa cagctacata tccatttttt gtattttgaa attccagttc caaattgtct
      721 attggttttt tctctataac agatacattc aaaataggaa ctacatcatc atttattata
      781 attttttctt tatttaacat ttctgtttta aactttcctg ttatattaaa attttctccc
      841 ttttccaact tttcataata atcagtaaaa tcccattcta catctatatc aagtcgatta
      901 ccactagatg tttctaatac catatatttg ggaaatttta tattttctat tttatcatct
      961 ttacttactt ttatttcatc actaaataca ccaattcccc tatgacaaca tttcacaata
     1021 tcatagccag aatcttcagc tatagaaggt ataagctcat tataatcatc aattatataa
     1081 actttcttac tttctgatat aattgattgt gccaacttat tattatctct tagtaaccca
     1141 ctttcattgt tgctactata tgcttttatt gaagtttctt ctatctcaac atcaccttta
     1201 gaataaacac ctatagcatg ttctccattc acttcaatat ccttatttag tattctaata
     1261 tcattttcag tataaattcc tattgcatct tttccatttg cctttatttt ttcaacagat
     1321 tcgtaggatg atttaatatg taagtctcct ccttcttcaa cgtataaggc tgtcccatca
     1381 gtagcaatta tgttgtttat accaatactt atacttccac cattttctat atgtattact
     1441 ccttcttttc ctccttcacc tattatattc aattcattat catcaataat aaatctccca
     1501 tgatttttta tcaatatttt atatttgttg gtatctattg taaaattact gtcataagga
     1561 atataaaatc taaactcttc atctattacc atatcacaag aaagagtata aactatttct
     1621 tctttattat ttttattttc ttgaacccac ctttcaaaat cttcaagaca attttcctct
     1681 tgtccaaaaa ctttatgtgg aaaacttata aaacacaaaa ctattatact cataaaaata
     1741 aatatctcat actttttcat cttacactcc tagaaatttt tagctttgaa acaatcctgt
     1801 cgaaacatat acttcaccca ctataatttt tccacaatta tcaattattg tcaatttata
     1861 taagtatctt tattaattgt tataaataaa aagctactta ctaaagcaaa cagacgtttc
     1921 actttaataa gtagctatat ttttataaca aaatagttat aatattagaa cttccttcat
     1981 ttacaatttt ttgtaaagtc ttttgcattt taactcttat ctcttctggc atagtatata
     2041 atttactttg taattgctct tttactaagt catgtagtga tttaccaaac atatttgatt
     2101 cccataaatc tgctggttgc tcttcaaata cttccataag atattttatc atctcttcac
     2161 cttgattttg atttccaact attggagaaa cctctgtaga tatatctgct tttataatat
     2221 gaagtgatgg tgcattggct tttaacttga taccatattg ttttccttgt ttcattattt
     2281 ctggttcttc cagactaagt tcttctaagg aaggagctac tactccatat ccttttatct
     2341 tggcatctat taaagcactt tctattttat catactcatt tttaacttta gatagtctag
     2401 ttatcagact aagcagttga taatctcctt caattttaaa tccacttttt tcttcaagga
     2461 cattatagaa taaatcttgc tttgtctgta aatcaatatt tataacacct tctcctagct
     2521 ctacattatc aacacctgta tcttccaaaa actctaactc agaaaatcct tgtataatac
     2581 cttctatatc ccttatttta cctatatcta taatactttg ttttaatgtt gttattatgc
     2641 tactttttat ccaatgattt ctttcaagac cttcaaccca ttttggtaag ttgattctta
     2701 tttctgtaag tggaaaatca tataatacag cttccataac ctcctctata tcttcttctt
     2761 ccatttcaac cacattcatt ggaagaactg gaacttcata cttttcttcc aattcacttc
     2821 taagcatact cgtttcctca ctttttggac ttaacgtatt taaaaccact gcaaatggtt
     2881 ttttaagatt tttcaattct tgtataactc tctcttctgg ttctacatag ctttttctgt
     2941 caattccagt aactgaacca tctgtcaaga caactatccc tattgtagaa tggtctttta
     3001 taactttttt agttcctatc tctgctgcct tttcaaatgt catagcttct tgtgaccaag
     3061 gagtggatac taatctttgt tttccacctt cctcatgccc taaagctcct tcaactatgt
     3121 atccaacaca atctaccatt cttactttta aagatactgt gtcttttatt tttatttcta
     3181 caccatctgc tggtacaaat tttggttcca ctgtcattat cgttttacct gaaccacttt
     3241 ggggtatctc atctcttgtt ctatcctttt taaattcatt gtctatatta ggtataacca
     3301 acttttccat aaattttctt ataaaagttg attttcctgt tcttacaggt ccaactactc
     3361 caatatatat atccccctga gtccttttgg atatatcttc gtatatgtta ttattcatat
     3421 tattcctcct atacatctaa tcttattaaa ttatatttag agttaagggt taatattctt
     3481 aaaatagaaa aataaagacc agcaatatta atttaattta catatagaaa tttgtaaatt
     3541 tcaatgtaaa ttaaattaat attgctggtc caaatagtag tgtattatat taactgactt
     3601 tagtagtatt ttcatttgaa ttattattca tctcaataag ttctttacgt ctttcttttg
     3661 tcatgttcat ccatataaat gtaaacacca atccaattat ggctactata gtaagaacta
     3721 tgaagtagta cctatatcca gttacacccg gaaatgcatc caatatcttc cctgctgcta
     3781 aaggacaaag tacttctgga agatatccta atgtagcaac tattcctata gcagttcctg
     3841 atattgataa tgaataatta ccttcttcaa gtaacgaata gtgtaaacct tgaattgcat
     3901 acatagatac atatattgca acacatccaa tcaatagtat ataaatcata gatgattttg
     3961 ttggtgtaaa tattacacct aaaagacctg caatcataag tataaatcca taagatataa
     4021 tctttgatga accaacttta tctccaagta atccagaagc agctgatgct actggtctac
     4081 aatactgagc taatattgag attgcagatg caaacaccac agtagctcca aatgcttcag
     4141 ttgaataagg cgtaaaataa taaaatgaca tattagctga atatgaacaa aacattatta
     4201 ttgttattat ccatgtatga ggcattttta ttactgcaaa aaaggcttct ttatcaaatt
     4261 ttgctgatac ctctttttca ttatccttta atttgaaaaa tactaatact ccaactatta
     4321 catttattgc cgagtataat actataatag ctgtaagacc agatttatca cttacttttg
     4381 atgaaatata cccaaatagt atcaatacta gagatagatg aattgcattg gtaattccac
     4441 gaccactttc cataaatcca aaagcttttc cttgttcatt ttcagatgct aacattctaa
     4501 cagcttttac aagagctggc caaaaagtaa gtattgttgt tattccccat aaggcatgga
     4561 ttaaaaacac aacaatatat ggaggataaa gtgttaatac aagaccagat attcctgtaa
     4621 taattaaaga acctgaaagc aattttctgg ctgaaatacg gtctgctaaa aaaccaccaa
     4681 aaaggtatga taccattgca agtagaccat aaatacttcc taagcttccc atttgtgtat
     4741 ttgtaagatt aaatgcttgt gcaaaagtat cataataata attctttaaa tatggtaaag
     4801 catatataaa tgctcctgct acaaagagta ttatcaagac tttgaaattt ttccaaatgc
     4861 ttggtttatt tacttcaact gacatacttc ctccttatgt aaatacctgt aaattttcat
     4921 gataattaat tattgcttat tgtattgttt attcaatatt tcggaatatt tattaatata
     4981 ttaggttaat ctatcaataa tttatggtga gttatgagct aaaagtaaac tcttttagct
     5041 caccataata attatatttt tatttatact aacgtccaga gttattagat atcttttttt
     5101 catagttctt ccatagcatt aaacctgtta ttataacaaa aagacctcct cctatcattt
     5161 caatctcata gattatatta tcttttaaag tctccataac tggtgccgtt gagaaaagta
     5221 tgccaactac agttactaca agtaccattc tggcaatatt gatacaaact gaatcacttt
     5281 ttgcaattga atatggtctt tcttcatttg gtcttgtctt tctaagtcta atataagatg
     5341 taaaaagcaa gacatatgga aataatgctg ttaatgctgt catagtaaca agaacattgt
     5401 atatattatc tactgatggt aataagtttg ttcctacaag aagtagactc actattatag
     5461 cctgaactat tactgcattt gatggtatat tgtatttatt tgtaacagtc aattgcttag
     5521 gaaatacacc ttctcttaca tttccaaaaa gcatctttac tggacctgct atataaagta
     5581 ttaatgctcc tagtactgat aaagttattc caaatgctac tattcttata aaccaacttc
     5641 ctatacctaa atcttgtgca actttagcta atgcatctag tatccctgta gatgctgcaa
     5701 ttttatctgg aggaagtatc attgttattg ctactgaacc taagatatag attcctccta
     5761 ttaaaacagc cacagttaat atagctttag gaaagttttt cttagcatta tccatttcgg
     5821 ttatgaaatt agcagtcgtt tcagctcctg ataatgcaaa tattatagca gatatagaaa
     5881 ctaatgaatt ggcatctatt tttggtatta tattttggat tgtatatatc gatgctgatg
     5941 gagctttttt gagaataact acagacataa atgccatcac tataagtaat atagcaggaa
     6001 ttgtagaacc taatgctcct gtattcgtaa aaaattttcc aaatgccatc ccacgagtac
     6061 ttactaagga caacagccaa aatattatga gtgaaagtgc taaaataaac atcttattgt
     6121 ttgataaatc tggatttcca agcatatatg caatatttat agctaaaaat gtcaagaaag
     6181 aagaatacca aaatatcttt gatgtccaat ttagccatga caccataaat ccccacttct
     6241 ctccataagc ttgtttaacc cattctccaa gtccaccatc ttttggatat gttgcagcca
     6301 actctgcaca tataagtgat gcaggaacaa agaatataaa agcaaacaat aaccaaactg
     6361 gtatagaacc aagacccaat ccaaaactag aggcagtaga tttagctatc catctaatcc
     6421 caaatagtgc tgatatattc ataaatacta aatcccaaaa tccaattttc tttttcactg
     6481 taattcctcc aatttcaaat ttattcaaac tcatacacta tgtattgatt taaatatcaa
     6541 gtataagttc ttcaacactt cttggataat tagttataat tgtagcacca ttcttaccta
     6601 ctattgctgt atctgagtgt ctaaatcctc caactccagg tatatatatc cctggttcca
     6661 tagaaaatac cattccttct tctaatatta attcattgtc aaatcttaaa tatggctctt
     6721 catgttctga caaacctaaa ccatgaccaa ttctatgatt tgaatataac tctagaccat
     6781 actttgcaac aacagctctt gctgcttcat ccaccattct tgctggtata ccagctttta
     6841 ttgtatctaa tccagcttgt tgagcctcta ctgcaatctt aaacacttct ttttgtcttt
     6901 ctgttggttt gcctataata aaagttcttt cattttctgc tctatagttt tcataccaaa
     6961 cctgtctact atgtattact atatctcctc tttgaagtat tctagtatta gaatctaaat
     7021 gaggttgtgc tgttctatca atccctgaac aagtccagtt tgcaaatcct atatatgtat
     7081 caggataatt ttcagaaaca tattttagta aagcattatc cccttcaacg tcaaactcca
     7141 attcactcat cccaactcta acattttcta aagacccttt tattcctata tcagataggt
     7201 aacctgctat cttcaaaaat tctatttctt ttgcatcttt tacatatctc atttcaaata
     7261 ttttacttcc aacatctttg atagcaaatg acttatcagt tatatactct gaaaagcttg
     7321 cagatactat atctttttct attccaacag ttgttccttt agggtattta tttaaaatta
     7381 tatctaagta atgtttatgt gatattccat gttctttcat ttctggtatt tcataataca
     7441 catatacatt atcaactttt gcaacttctt tagcatgtaa ttcttctaat ccaggtacta
     7501 ttaattctat tttatcttga gttactactg ttacaattgg tctagaataa gttattgctt
     7561 taaatccact aatataaaat tgattgtctg gttcaaatat taaagcaatt tcaatgcttt
     7621 tttcattcat gtactttctc aatctttcta gtctcatatt atttgacata atgcacctct
     7681 ttcttaatat tcagataatt tattcaatac aacaaggatg aatatattat tttaaaattc
     7741 ttaatatttt tagtacttgt cgtatttgta tgtaaattaa ttttatttgt gattatttta
     7801 tcataatcat gattattttg tcaatcatga ttttatttta aaaaattttt tatattctag
     7861 tgatt
//
LOCUS       DACULQ010000164         7858 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.165, whole genome shotgun sequence.
ACCESSION   DACULQ010000164 DACULQ010000000
VERSION     DACULQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.165"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            1..747
                     /locus_tag="JA154_15210"
     CDS             1..747
                     /locus_tag="JA154_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7997176.1"
                     /translation="MNNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGLVFKRFDV
                     RGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDDAKSKGKY
                     GLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPCFKNCVKQR
                     KLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAPAPFTTYS
                     LFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    965..1178
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1432..2466
                     /gene="argC"
                     /locus_tag="JA154_15215"
     CDS             1432..2466
                     /gene="argC"
                     /locus_tag="JA154_15215"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088548.1"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis and the reduction of
                     N-acetyl-gamma-aminoadipyl-phosphate to
                     N-acetyl-L-aminoadipate-semialdehyde in lysine
                     biosynthesis; involved in both the arginine and lysine
                     biosynthetic pathways; lysine is produced via the AAA
                     pathway, lysine from alpha-aminoadipate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAT7997177.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKIASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2507..3724
                     /locus_tag="JA154_15220"
     CDS             2507..3724
                     /locus_tag="JA154_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine biosynthesis protein ArgJ"
                     /protein_id="HAT7997178.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEAHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3739..4599
                     /locus_tag="JA154_15225"
     CDS             3739..4599
                     /locus_tag="JA154_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAT7997179.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4629..5816
                     /locus_tag="JA154_15230"
     CDS             4629..5816
                     /locus_tag="JA154_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HAT7997180.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYVERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            5925..6947
                     /locus_tag="JA154_15235"
     CDS             5925..6947
                     /locus_tag="JA154_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAT7997181.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7074..7691
                     /locus_tag="JA154_15240"
     CDS             7074..7691
                     /locus_tag="JA154_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997182.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
BASE COUNT         3185 a          749 c         1475 g         2449 t
ORIGIN      
        1 ataaataata ttgacaatga acatatatgt tgttcaattt ctgaaaaaaa aggagaaaat
       61 tgtgtttttt ctaaaaaagc ttggatgaaa aataattttg ataaaggtct agtctttaaa
      121 cgatttgatg taagaggtaa agcttttata gaatatattc cagcagaaaa tgcatggtgt
      181 cctattactg cggacaatta tatatttatt aactgttttt ggatatctgg aaaatttaag
      241 ggtcaaggat attctaatct attgctacaa gagtgtgttg atgatgcaaa atcaaaaggt
      301 aaatatggat tagttgtgct ttcgtctagt aaaaaaatgc cttttttatc tgatgctaaa
      361 tatctaaaat ataaggactt ttgtttagca gatacatcag aaccaaatta tgagctatta
      421 tattttccct ttactgataa tgctcctatt ccatgtttta agaattgtgt aaaacaaaga
      481 aaattaaatg agaatggtat agttttatat tacagtaatc agtgtccaca tacggaaaaa
      541 tatgctccta ttttgaaaaa aattgcaaat tctagaggta ttgatgtaaa tcttataaaa
      601 ttcgaaacag caaaagaagc acaaaatgct cctgcaccat ttactacata cagtcttttt
      661 tttaatggtg aatttgtaac caacgagata ctttctgaga aaaagattga gaaattttta
      721 gataccaaca ttaaaaaatt taattaaaaa gagatgtaaa tattgtattt tgaaaatata
      781 ttattttata atttaatata ttttggatgt tttttaaatt gacgaatgaa atataaaaat
      841 tatatcgcaa tttagataca aaatgctgga aattaaccat atagaaaata tttccaaaaa
      901 tttaaaaatt atattgacaa agttaaaaaa ataaactatc atataaaaca ataagaaaat
      961 aaattgaaat cctgtgaaaa agagagtagc ataaagctag ttgatagaga gctgggaatg
     1021 gtggaagcct agtaacgaat tttattgtga atagagcttt ggaggagatt accagaagta
     1081 atagtatggt aattggtgaa tctcgcctta agagattaga gtgggtaaga ttatcttatc
     1141 aattagagtg gtaacgcggg tgtatattcg tctcttggtc tttgaccaag aggctttttt
     1201 aattttaaat gtacattttg tagatacttt ttaatttgag ttttaatgat tattgtaatt
     1261 ttaccaataa gagtggtacc gcggaaaatc gcctcttagt atttataatg ctaaggggtt
     1321 ttttgtttta attagagtat tttaatctac gtattatcaa atgattatca tatagtaaag
     1381 tggaaagata ggattttaag actatttgaa tataaaaatg gagggaacaa aatgaataaa
     1441 ataaaagttg gtattattgg tgcaactgga tatgtcgggg cagagttgat tagattatta
     1501 atgaatcatg ataaagtcga agtgacagcg ataggttcaa attcgtatgt tggaaaagat
     1561 atagttgata tatatccaag tataggatat aaaaataaca tgatatgtat agaaaatgaa
     1621 aaagttatag atatgtgtga tgttgtgttt acagcccttc cacatggagt tagtgaaaag
     1681 tttgtaataa aggcaattaa atcgaaaaag aaagttatag atttaggtgc tgactttaga
     1741 ataaaagatg aagaagtata tagtaaatgg tatggagtat cttttataga taaaatctta
     1801 cataaaaaag ctgtatatgg attaagtgaa atatacaaag aagatataaa agatgcagat
     1861 ataattgcaa atcctggatg ttatccaact agtatttctt taccattgat gccattacta
     1921 agttctaaat tgataaaaaa taataatata ataattgatt ctaaatcagg tcttacaggt
     1981 gctggtagag aattatcgat tagtagtcac tttacggagg ttaatgaaaa tatcactgca
     2041 tataaaatag ggaaacacag acatactcca gaaatagaac aaaatttaag tgaatcctgc
     2101 aaagaaaaag taagtgtagt ttttacacca aacttaatcc cagtaaatag gggaatctta
     2161 tcaactatat attgtacaaa agaagataat atttctataa atgatataca taggaaatta
     2221 actgattatt atgaatttaa agaatttata gaaattcttc cattagataa gatagcttct
     2281 ttaaagaatg ttagattctc aaataaatgt gtaatatcat tacatgaaaa tggagataca
     2341 ttgataatct gttcagctat agataatatg attaagggag cagcaggtca agctatacaa
     2401 aacatgaata taatatttgg tattgaagaa aatacaggtt taaaaaatat tgcaccatca
     2461 ttttagaaat aaaaaatact aaactcatat aattaggagg ataaatatgg agattataaa
     2521 gggtggagtt acagtttcag aaggattttt tgcttcaggg attcactgtg gcttgagaaa
     2581 aaataaagaa aaaagagatt tagctttggt ttattcagat gttttatgtg atgcagcagc
     2641 agtagtaact caaaataaag taaagggcaa tcctgtttat gttacacagg aacacattaa
     2701 aaatggaaaa gcacaagcta tcatagtaaa tagtgctaat gctaatacct ttaatggaaa
     2761 agaaggactt ataaatgctt ataaaatggc taaattcacc agtgataaat taaatattaa
     2821 agaaagtgat gttttggttg catctacagg tgttatagga aagccattaa atatagattt
     2881 aatagaagaa aatatggatg aattagtgaa taatctaagt aaacaaggtc atattggagc
     2941 aagagaagct attatgacaa ctgatattat aaaaaaagaa atagcagtag caataatgta
     3001 tggagataaa aaaataacta ttggaggtat ggcaaaaggt tctggtatga tacatcctaa
     3061 tatggcaaca acgttaggat ttataactac agatgcaaat attgatggag ttttgctaaa
     3121 agaagcttta aaaattgctg ttgacaaaag ttttaataga gttagtgtag atggagatac
     3181 aagtactaat gatatggtaa tgattctagc aaatggaaaa gcaaaaaatg atagaataaa
     3241 caaaaaagat gaacattatc aagtattttt gagtgcactt acatatgtct gtatagaact
     3301 tgctaaactt gttgcaaaag atggagaagg atgtacaaaa ctaattgaat gctatataaa
     3361 tggagcatta agtgaagcac atgcagtaaa attagcaaaa acagttatat cttcttcatt
     3421 agtaaagaca gctgtatttg gagctgacgc aaattggggt agaatactgt gtgctttagg
     3481 ttatgcaggt gaagaaatag atatggaaaa agttgatatt atttttgaga gtatgaaagg
     3541 atatattgaa gtatgtaaaa atggaaatgg attagatttt aatgaggaaa aagcaaaaaa
     3601 aatccttgaa gatgacgaaa taagtatatt agtagattta aatatgggca atgccagagg
     3661 aaatgcatgg ggatgtgatt taagctatga ttatgtaaga ataaatggaa gttatagaaa
     3721 ttaggaggat atactttaat gattaatata gaaaaagcaa atacacttat agaagcttta
     3781 ccatatatag aaaaacatca gggaaagact attgtagtta agtatggtgg aagtgcaatg
     3841 aaaaaagatg gtctaaaaga atctgttatg gaagaccttg tgttaatgag ttatgttgga
     3901 atcaatatag ttttagtaca tggtggaggg gcagaaatca ataagatgct agcaaaagtg
     3961 gatatagaaa gtaaatttgt aaatggtctt agatatacag atgaagagac catggaaatt
     4021 gtaaaaatgg tacttgctgg aaaggttaat aaagacctag taaataaaat acatacaaaa
     4081 ggtggaaaag cagtagggct ttgtggaata gataataata tgattttatg tgacccatat
     4141 aaaaattatg aacttggatt tgtaggagaa atcaaaaaag taaatgttga gcttatagaa
     4201 tcttgtctaa agtcaggtta tatttcagta attgcaacta taggtgttgg agatgatgga
     4261 gagacttata atataaatgg tgacacagca gcttctgcta tagctaaaga gttaaatgca
     4321 gataaattga tacttcttac agatgtccct ggtttgttaa gagagccaga tgaagaaaaa
     4381 tcattaataa cagaagttat attagaggat gttgataaat tatttgaaga aggtataatt
     4441 actggaggta tgatacctaa aatagaggga tgcgtagatg ccttaaataa cggagtaaat
     4501 agggtacata ttttggatgg aagagttcca cattctataa taacagagct atttactgat
     4561 agtggaatag gtacattaat aagaaaagaa aatgaataaa attataagtt taagagggga
     4621 gaataagaat gaataacgac aatacaattt caaaatggaa tgaatatttt atagatacat
     4681 ataatcaacc caattttgtc atagattatg gtgaaggaag ttgttttttt gatactaatg
     4741 gcaataaata tatagatttt acaagtggat atggagtatc ttctcttggg tatagtaata
     4801 gtaatttaaa gaatgctcta aaagagcaag tagataaatt attacatact tcaaatttat
     4861 attttaatga gccagtgttg tattcaggag agaaaataat taattcaagt ggaatggcta
     4921 aagtatattt ttgtaattct ggaacagaag caaatgaaac agcattcaaa atagcaagaa
     4981 agtatagtag tgacaaatat ggtaatggta gaggaactat cataagttta aaagactctt
     5041 ttcatggtag aacaatgatg tctttaatgg caactggtat ggacaaatac cataagtatt
     5101 tttatcctct tcccgaaggt tttaaatatg tagaaagaaa taatattgaa gatttaaaaa
     5161 ataatcttga ttccacagtt tgtgcaataa ttcttgaagc gattcaaggc gaaggtggag
     5221 ttaatgttct tgaaaaagat tatgttttgg aaatagtaaa aatatgtcaa gaaaaggata
     5281 ttgtagtgat ttttgatgaa gtccaatgcg gtataggaag aactggtaaa ctttttggct
     5341 atgaatattt tgatgtaaaa ccagatatag ttaccgtagc aaaagggttg ggagcaggaa
     5401 taccagtagg aggagtatta gtaaataaaa aactttcaaa agtactagga aaaggagacc
     5461 aaggtacaac ttttggaggt aatttattag ctatggtggc agcttcagtt gtattagatg
     5521 agatatctaa agatggtttt tataatgaag tattagaaaa aggaaattat ataagaaaat
     5581 ctattgaaag ttttaataac aaagttgtat taaagacaaa aggtattggt ctaatgatag
     5641 gaattgaaac taatattgaa tctagtatta tagaagagaa agctagaaaa aaaggattgc
     5701 ttattttgac tgctggaaaa aatgtattga gatttttacc accattaaca atcagttata
     5761 aggaaataga tgaggctctt gaaatcctaa aagatatatt actagaaatt aactaaatat
     5821 tcaagaaata gttgattaaa gataaaaatt agaagtaaat aaaaaagtaa agtgctaaca
     5881 agtttaattg aatttaaaaa gtttataaga aggggcgaat taatatgaat aatataagtt
     5941 taaaaggtaa gagttttttg acattaaagg attttacaaa agaagagatt agatatttat
     6001 taactttatc aagttattta aaaactaaga aaaaagttgg tataaaaggc gatttgctac
     6061 aaggaaaaaa tatagcttta ttatttgaga aaacatcaac aagaactaga tgttcatttg
     6121 aagtagcagc tcatgatgaa ggagcacatg taacttatct aggtccaaat gattgtcata
     6181 tgggtaaaaa agagtctgta tcagatacag caaaagttct aggaagattc tatgatggaa
     6241 tagaatttag aggctttaaa caagaaacag ttgaaagtct agcaaaatat tctggtgtgc
     6301 ctgtttggaa tggacttaca gatgaatatc atccaacaca aatactggca gattttttaa
     6361 cagtaattga aaatgtagat aaagatttga ataaagttaa atttgtatat gttggtgatg
     6421 ctagaaataa tatgggaaac tcactgatga taggatgtgc taaaatggga atacatttcg
     6481 tagcactagc acctaaggaa ctttggccaa atgaagaatt ggttaaggaa atgaaggaaa
     6541 tagccaagaa atccaaagga gatataatgt tgactgaaaa tattgatgat gttaaaggtg
     6601 cagatgttat atatacagat gtatgggttt ctatgggaga agaggagcaa tatgaagcaa
     6661 gaataaatca acttaaagat tatcaagtaa atatggatat gataaagaaa actgaaaatg
     6721 aaaatgttat atttttacac tgtcttccag catttcatga tttagaaact aaggttgctc
     6781 aggagattta tgaaaagttc ggattatcag aattagaggt tacaaacgag gtatttaatt
     6841 caaaatattc aaaggtattt gaagaagctg aaaatagaat gcatacaata aaagctgtta
     6901 tggtttctac tatgactgat gtaaatatga acaataattt aatatagata tgttttaata
     6961 tagactttac tttaatgaaa atttataaaa tattttaaat atattattca aaaactctca
     7021 ttatatgtta aaataactta tgtatcaaca aattaagagg gaggatttga gatatgaaaa
     7081 aaatcatatt agctttaaca acttttttat tagcaatatc tttaacagca tgttcaagtg
     7141 caaaaccaga agatacaatt gatagttttt ttaacagtgc gaaaaagttt gactttgaag
     7201 gcatgaataa ggtaatggaa aataatgacg aaaaatataa ggatatatta aaggaattag
     7261 atacaaaaga cccaaatgcc caatatgttt tagattatct aaaacagaat gcatctaaaa
     7321 tcacatatac aataaaagac agcgaggtta aaggtgataa ggctacaatc aaagtagaat
     7381 gtaaatttat agattcaaca ccattacttc aagaaatagt agctgaggca tttacaaaga
     7441 tgattggtat gagtttttca ggacaagact taactgatga gaaaacaact gaaatgttag
     7501 tatcaattat gaaagaaaaa caaaaaagcg ttaaagagac ttatgttaca aaaaatgtag
     7561 agtttgaatg ttctaaaaag gataataaat ggataataag ttcagtaaat gatgcagtag
     7621 ctgatgtatt gttatctaat cttgttacag caggacaaga attttctaac tctatgaata
     7681 cacttgaata atattgttta ggtgtagtaa agtaataaat atatgattat ttttataaat
     7741 aaacatataa atatttatga aggataccat ttttggtatc ctttttatta atataatgaa
     7801 atacatggtt aatttctata ttgtcaagaa actatgatat atgttataag tctatact
//
LOCUS       DACULQ010000165         7757 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.166, whole genome shotgun sequence.
ACCESSION   DACULQ010000165 DACULQ010000000
VERSION     DACULQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.166"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            308..379
                     /locus_tag="JA154_15245"
     CDS             308..379
                     /locus_tag="JA154_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997183.1"
                     /translation="MLNFITKKEGMIQNGEVNRQETG"
     gene            390..1517
                     /locus_tag="JA154_15250"
     CDS             390..1517
                     /locus_tag="JA154_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997184.1"
                     /translation="METFIFLILLVVGFGYVGSIMGAGMMFKVIMSTAHALLLETVFL
                     IMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIAGAITTYLSDNPAII
                     PFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEYVLPALIGNLGAIIG
                     SVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVFQRALDAILEGGKMG
                     VDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGIKLLPVIGDKLGFIL
                     EPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIAVFTAMGMCWSGYLS
                     THIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            1897..6150
                     /locus_tag="JA154_15255"
     CDS             1897..6150
                     /locus_tag="JA154_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HAT7997185.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            6299..6793
                     /locus_tag="JA154_15260"
     CDS             6299..6793
                     /locus_tag="JA154_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAT7997186.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            6909..7286
                     /locus_tag="JA154_15265"
     CDS             6909..7286
                     /locus_tag="JA154_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAT7997187.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            7492..>7757
                     /locus_tag="JA154_15270"
     CDS             7492..>7757
                     /locus_tag="JA154_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HAT7997188.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDK"
BASE COUNT         3115 a          792 c         1396 g         2454 t
ORIGIN      
        1 tataaaacta aaaaaaaaga ttatgaaaaa tattgaagga aaaatttttt cataatcttt
       61 tttctttcat gcaaaaagat acaagttaca acttatattt gcatattagg tcgttagaga
      121 agtatatata tatttagtaa ggaatcatac tttttataaa atgaaagata agatgtcgaa
      181 aacttgcata aatctgaaaa atttttatta ttaaaatact gatttccatt ttaaaaatga
      241 taaaaaaatg acaaataaag ttgcaaatag taatacttat aagtataatt aaaaaagaga
      301 aaaggttttg ttaaacttta taacaaaaaa agaagggatg attcaaaatg gagaagttaa
      361 cagacaggag acaggttaag gcagtaagca tggaaacctt cattttccta atattactag
      421 tagttggttt tgggtatgtt ggtagtatta tgggagcagg aatgatgttc aaagtaataa
      481 tgagtacagc acatgcttta ctattggaga cagttttctt aattatggct atggcagttc
      541 ttgcaggcgc attaagcgca ctactttcag agtttggagt aatagcgtta attaataaaa
      601 ttttcgctgt atttatgaag cctttatatg gactacctgg tgctagtata gcaggagcta
      661 taacaactta tttatcagat aatccagcta ttataccttt tgctaaagat aaaacattca
      721 cacagtattt taaacaatac caagttccag cattatgtaa cttaggaaca gcctttggta
      781 tgggacttat cttaacaaca tttatgattt ctcaaggtac agaatatgta cttccagcat
      841 taattggtaa tttaggtgca attataggta gtgtcataag tgttagaata atgttgacat
      901 ttactaagaa gttttataaa tacaatccgg aagaagataa agcaacagga acattagaga
      961 aaaaggatga atttagagaa atcagagaag gaaatgtatt tcaaagagct ttagatgcaa
     1021 ttcttgaagg tggtaaaatg ggggtagata tgggaatggc tataattcct ggagtcctag
     1081 tagtatgtac attggtgatg ttattaactt ttggaccatc tacagatcct gttacaggtc
     1141 aagaagttta tacaggagct gcatatgaag gaataaaatt acttccagtt attggagata
     1201 aacttggatt tatattagaa ccattatttg gatttacatc accagaggca atagcattcc
     1261 ctataacttc attgggagct gttggcgcag caatgtctct agttccagag tttataaagt
     1321 caggagctat aactcctaat gatatagcag tatttacagc tatgggtatg tgttggagtg
     1381 gatatttaag tacacatatt ggtatgatgg atgcattaaa tgctagacag ttggcaggta
     1441 aagctattct ttctcataca ataggtggtt tatgtgcagg agcagctgct cactttatat
     1501 ttacactcgt aggataaatg taatcagcta agataaaaaa taatatacaa aaaatggttg
     1561 ttgatatgta ataagaattt acatatcaac aaccattttt ttctgactaa agataattta
     1621 taatacataa acattaagtg gatattagag ttaagttata tttatgtaaa aagtaacaaa
     1681 aatctgtaga aatttatgac aaaatttagt acactaacta tatgttctta tttaaaattt
     1741 aaggttagaa ttaggaatta cctagtagag taagctttga ttaataaaag aatcataaaa
     1801 taaaaatatt agtcataaag attataaata acttacttaa aataaagcca tagaacttac
     1861 aaaaattaaa aaagcatata aatatggagg ggtacaatgg atgaattatt tcatatagga
     1921 attgatgtag gttctacaac ggtgaaggtt gtggtactta ataatagtaa taatatagtt
     1981 cataaagaat atagaagaca ttattctgat gtaaaaaaat cagttaaaga agtactaaat
     2041 ggtatatatg aaaaattggg agatataaat actaccatta taataactgg ttcaggtgga
     2101 attggaatat caaaaaaatt aggtgtaaaa tttgttcagg aagttatatc tagtacaaaa
     2161 tcaatagagt attttcatcc tgaaactgat gttgtcatag aacttggggg tgaagatgca
     2221 aagataacat atctaagtgg aggaatagac caaagaatga atggaatatg tgcaggtggc
     2281 actggagcat tcatagacca gatggcatca cttttaaaga cagatgccag tggattaaat
     2341 gaacttgcta aagggtataa tgttatatat cccatagctt caagatgtgg tgtttttgct
     2401 aaaacagata ttcaacctct tataaatgat ggagccaatc agactgatat agcaatgagt
     2461 attttcaatg cagttgtcgt acaaactgtg agtgtccttt cttgtggaag aaaaatagaa
     2521 ggaaatgttg cgtttttggg tggtccattg tactttttat ctgaacttag agaagcattt
     2581 aaaagagtat tagatttaaa agatgaaaac ataatcttcc cagaaaatgc acaactttat
     2641 attgctattg gagcaggatt attatcagta gatgaagata atatacaact aaaatctcta
     2701 atagaaaaat tggatagtat aaagaatatt gaagatggag aagtaaattt attagaacct
     2761 ttatttaaag ataaaaatga gtatgaagaa ttttcaagaa gacatgaaaa agaaaaaata
     2821 aattatgtgg acataaattc aataaaaagc aattgttatt taggaattga tgcaggctca
     2881 actacaacta aagcagcact tattgatgag gatggaagac ttgtatattc ttattataat
     2941 agcaatgaag gaaatccatt aaaaacaacc attaaagtta taaatgaagt atatgatata
     3001 ttaccaaaaa atattaaaat attgagttca actgtaactg gatatggaga aggtcttatt
     3061 aaaaaagctc ttaaaatcga taatggagag atagagacta tagcacatta caaagctgct
     3121 aaatttttca acaaagatgt agattttata cttgatattg gtggtcaaga tatgaagtgc
     3181 ctaaaaataa aagatggagt aattgataat ataatattaa atgaggcttg ttcatcaggt
     3241 tgtggctcat ttttagagac atttgcaagt tctttatcta tgacaataga agaatttgcg
     3301 catgaaggta tttattctca aaatcctgta gatttaggtt caagatgtac tgtatttatg
     3361 aattctagag taaaacaagc tcaaaaagaa ggtgcaagtg ttggagatat atcagcaggg
     3421 ctatcttatt cagtaataaa aaatgcttta tttaaagtaa taaagattag agatttaaat
     3481 gagattggta ataatattgt tgtacaaggt ggaacctttt ataatgattt ggtgcttaga
     3541 agctttgaaa aactaattgg aaaaaatgta attagaccta atatttcagg aattatggga
     3601 gcttttggtt cagcacttat agctaaagaa aaatatgaaa gtggatacaa aacatccttg
     3661 ttatctaagg aagagttgaa taatatcaag ctagaagcaa gtgtaactag atgtaaaggt
     3721 tgttcaaatc attgtcttct tacaataaat aaattttctg ataatgaaat ttttatttca
     3781 ggtaatagat gtgagaaagg tgaagctatt tatggtgata aaaaaatagg attagaaaag
     3841 aaaaaaccaa taaatttatt taagtacaaa tacaatagaa tttttagata caaaccattg
     3901 gaaaaagaag aagcaaaaaa tggggaaata gggataccaa gagtattaaa tatgtatgaa
     3961 gattatccat tttggtttac ttttttcaat gctatgggct ttagagttgt actttcagat
     4021 aggtcatcaa aacagttata tgaaactggt ataactagta ttgcatctga aacagtttgt
     4081 tatccaggaa aattagttca tggacatata gaaaatttaa ttgcaaaagg tataaaaaat
     4141 atattttatc caagtgtaac aaatgaaaat aaagaggatt caaatgcaga taattattat
     4201 aattgtcctg ttgtaatatc atattctgaa gtaataaaaa acaatgtaga gaatataaga
     4261 aataagaaca taaattatat aaatccattt ataagtctaa atgacaagga aaaattgaag
     4321 aaaagactct atgatgaatt atcaagacat ttttctgata taaaaataac taaagaagaa
     4381 gttaatcatg cagttgataa agctactgat gaacagaatt tatttaaaat ggagatacaa
     4441 gcagcaggtg aaagagcact taaagaaata aaagaaagaa atatgaaggg gattgtgtta
     4501 tgtggaagac catatcatat agacccagaa ataaatcatg gaatgccaga gcttataaat
     4561 tcattagata tggcagtgct tactgaagac tctatatgtc atttagccaa tgtacaaaga
     4621 ccacttagag ttgtagacca atgggtatat cattcaagat tgtataaagc agctagtttt
     4681 gtaagagata aaaactattt ggaacttgtt caacttaatt catttggctg tggattagat
     4741 gcagtaacta cagaccaggt tcaagaaata cttaatgaaa aatcgaagat atatactatt
     4801 ataaagattg acgaaggcaa caatttagga gcagctaaga taaggatgag gtccttaaaa
     4861 gcagctatgt ttgagagaga aagcaaaaat atagatgtaa aaaatataaa ggtaaacagg
     4921 atacagtatg ctaaaaataa tcagataacg aacaaacata ctatattagc accacaaatg
     4981 tctccaatac atttccaatt ccttgaaaaa gctgttaact taagtggtta taatatagaa
     5041 attctaaaag atactgatag tagtgtaatt gaagaagggt taagatatgt aaataatgat
     5101 gcatgttatc cagctataat agttgtaggt cagctaataa cagcattaaa atctggcaaa
     5161 tatgatttaa ataacacttc tgttactata actcagactg gtggaggttg tagagctaca
     5221 aattatattg gattcttaag aaaggctata tacgatgctg gatttaaaga tattcctgtc
     5281 attgcactta gtgtaaatgg aatagaagat agtggtataa tggataatat atcactaaaa
     5341 ttaatcaata gactctttat gtctgttgtt tatggtgatt tgctgatgaa agtactatat
     5401 agagtaagac catatgaaaa agttactgga agtgcaaatg ctctatatga aaaatgggtt
     5461 gatatatgca aatcttctct tgttaaagca aaaatttctg tatttactag aaatataaaa
     5521 gatatagtaa aagaatttga taatttagag atactagata tcaaaaaacc aaaagtaggt
     5581 ttggttggag aaatattggt gaaattccat ccaattgcaa ataataattt agtagatata
     5641 ttagagcgag aaggtgctga agcagtcgtg ccagacctta caaatttctt cttaagttgt
     5701 gcctttaata ctatatacaa acacactcat ttagaaggaa gtagaaagag tagaatgata
     5761 ggagaagcat ttatatacat aactggaata tatcaaagag tatataaaaa agcacttgat
     5821 aagagtgaga gattttatgc tccagccaat ataaaaaatg ttgctaaaag tacagaacca
     5881 gtagtgtcat taggtaatca aactggagaa ggttggcttt taacaggtga gatggttgaa
     5941 ttattaaatg aaggtgtaga aaacattatt tgtatgcaac cttttggatg tttacctaat
     6001 cacataattg gtaagggttc tataaaagaa ttaaagaggt tgtataaaaa tgcaaatata
     6061 atacctattg attatgaccc ttctgcaagt gaggtaaatc aacttaacag gataaaactt
     6121 atgctttcta aggcttttaa aaatatataa gatttatatt ttgatatgtt aaaagttaat
     6181 gaatttaaaa atatactcta tattaagtat ctataaaaaa gagttatcct attttttagg
     6241 gtaactcttt attagttaaa aaattatatg tatactgata ttaaaggggg gaagtaatat
     6301 gagtttagta tatgatgttt ataaatcaaa attaggaaat ttgtacatat taagtgaaga
     6361 tggttttata gttagtatat acataggaga tgaaaaattt aataatttaa aaaatgaatg
     6421 tattaaaaaa ggaattgata taaagagaag taaggaagat ttaaaggaag caataagcca
     6481 gatagatgat tactttaatg gaaaaagaaa agtctttaat ttaaatattc gtattgaagg
     6541 gactgaattt caaaaatcag tttataggga aatgttaaaa ataccttatg gagaaacatt
     6601 gtgttattca gatatagcaa gaaatataaa caatccaaaa tcagtaagag caataggaca
     6661 agcaagtaaa agtaataaaa ttccaataat aataccttgt catagagtag ttggcaaaaa
     6721 agatattggt ggatatatgg ggaatcattc tgatttgaaa gagatactat taaatttaga
     6781 aagacagagt taacttagaa agaaatgttt ttattgttaa tatttgatat aataatagta
     6841 aaaagaagta ttttttacaa aaacaatgaa gaatttattt taatttataa aaatgagggg
     6901 gaattttaat gtgtgataaa gttatagcat caaaatatca tgagaaggga tttaattgtg
     6961 cagaatcagt tataaaagca tataatgagg aatttaatac agatatacca gtttgtttag
     7021 gaagtggact gggttcaggg tgtggggttg ctagtttatg tggtgcagta aatgcatcaa
     7081 atataataat tggatatgta aaaggcagaa accacgaaga tgaaagtact aaagctaaag
     7141 tatatgctaa agatttaact acaacagtaa gaaaagagta tggttcagag ttatgtatag
     7201 acctaaaaaa agatttagtt gcttgtagag aaataatgga tttcgcatat gatagtctaa
     7261 aggaaacttt aaaaaaagaa ttataagaaa ataagctatc aagtataaat tatttgatag
     7321 cttattttta tagtcgatac taaataaatc aaatatattt tagaattatt atttgtaaat
     7381 aaacagcaaa taattttagg atttttattt aatatagttt ttatttatat ttatttttga
     7441 atatgttata ctttgtttaa ataaatattt gtaatgaggg agatgagaag attgaagaat
     7501 ctcaatttac ttattaataa attatattca aaaaatcata atgaagcata taaaactttt
     7561 ttattcttag aaaatgaaag tttaaaatca aatattacat attgtttttt tgactcattc
     7621 ctagaaatga ttgataacga aaactcatat attcgaacaa ggggattgtt attaatctct
     7681 gcaaatgctc agtgggatat tgataataaa attgaaataa atatagatag tatactaagt
     7741 catatcgtag ataaaaa
//
LOCUS       DACULQ010000166         7724 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.167, whole genome shotgun sequence.
ACCESSION   DACULQ010000166 DACULQ010000000
VERSION     DACULQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.167"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            154..1212
                     /locus_tag="JA154_15275"
     CDS             154..1212
                     /locus_tag="JA154_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAT7997189.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1307..2500
                     /locus_tag="JA154_15280"
     CDS             1307..2500
                     /locus_tag="JA154_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAT7997190.1"
                     /translation="MKNLKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILID
                     QETGRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSS
                     YKLKDGEEVPLIELMRGLMIVSGNDAAIAIAKHVCKTKEAFVDRMNKKAKEIGMSNTH
                     FLNPNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDR
                     SFEKSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTN
                     HKNKRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGTKELEVTLKAKDELYTILK
                     DDESIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLK
                     MKFAN"
     gene            2707..3420
                     /locus_tag="JA154_15285"
     CDS             2707..3420
                     /locus_tag="JA154_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAT7997191.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3499..4410
                     /locus_tag="JA154_15290"
     CDS             3499..4410
                     /locus_tag="JA154_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAT7997192.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4495..5199
                     /gene="pyrH"
                     /locus_tag="JA154_15295"
     CDS             4495..5199
                     /gene="pyrH"
                     /locus_tag="JA154_15295"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088652.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAT7997193.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5225..5782
                     /locus_tag="JA154_15300"
     CDS             5225..5782
                     /locus_tag="JA154_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAT7997194.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5785..5988
                     /locus_tag="JA154_15305"
     CDS             5785..5988
                     /locus_tag="JA154_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997195.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            6017..6751
                     /locus_tag="JA154_15310"
     CDS             6017..6751
                     /locus_tag="JA154_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAT7997196.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6764..7543
                     /locus_tag="JA154_15315"
     CDS             6764..7543
                     /locus_tag="JA154_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAT7997197.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
     gene            7518..>7724
                     /locus_tag="JA154_15320"
     CDS             7518..>7724
                     /locus_tag="JA154_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997198.1"
                     /translation="MRLNFLLDKDWLDLKEQSIIASNENSYVTFKCRDILQLLVTWLF
                     SLFKNLEDLGELYKNRYYFIIVYYI"
BASE COUNT         3195 a          793 c         1460 g         2276 t
ORIGIN      
        1 tatgaaacaa attaaaagca caatgaagta gattaaaaat ttaaaactaa gaatctaata
       61 tatattttgt aactatttaa gtatataaaa taaaataata tccaaactaa tacaaaatag
      121 taataattgt tttgtactgg aggatatata tttatgaatt gtaaatggat atctaaaata
      181 gatgagagaa aaaaatgtca tagagaagca gattcttctg gatattgtat ctttcataaa
      241 gaaaataagt ctgatgaaga gatacaattg atgatggata ctcttcataa agaagagata
      301 agtgaattta atggatttgt ttttgaaaat gaatttaatg cggaagaaat attaacttac
      361 aattataaaa tactggattt ttctgaatcc atatttaaac aaaaagcaaa ttttaaaaaa
      421 tatattttta aaaagaatat tatttttaac tatacagaat ttagagataa agttttattt
      481 aatggttgtg tattcttaga aaactgtgat tttaatagaa ccatatttag taaacactat
      541 ataaatgacc gtatatttga aaaagtgaaa tttaaaggac ctgatttggt agttaataaa
      601 gtagaaaatt ttccacgaat ggatggaata atttttagca tgtgtacaaa atttgtgtta
      661 aagaatgttg aatatggaaa aagtgagtat gagcatggca agataaatta tagaatagct
      721 agaaatcaag caacaaaaat tggagaatat gagatgatag gattttacta ttataaggaa
      781 agaatttata gtagtaaaat aatgaaacgt tctaattatc ctaccttcag tgattattta
      841 gttgaaaaat tttttgacca aatagcacgt tacacaacag gttatggaga aaagccatgg
      901 aatatattat tagttattat agcaataata agtgtttttg ctttattata cttatttgta
      961 ggcatagaaa gttcaaatag cacacttgta gctttagata taaataatat aggtgattac
     1021 tcactaagtg agattttcaa aatgtatatg gatttatggt attttagcat ggctacattt
     1081 agtacagtag gatatggaga tatggttgct acaagtttga ttgggaaagc attggcaggt
     1141 atagaagtat tttttggagt tacaatagga gcaatttggg catctgtgat tattaaaaga
     1201 atgataaggt aatatttgtg taaatatgga taggtattaa ttactagata taaattggac
     1261 taaataggga aaatttaaat ataaatatac ttaataatcc aagaaggtga aaaatttgaa
     1321 aaatgcacta aaaaaaatta tatcattagc catgattcta gtttttataa taccaaatta
     1381 ttcatatgca gatgataaat ctgtagacaa gtattcaaaa acatctatac ttatagacca
     1441 agaaacaggt agagttttat atagtaaaga acctgatatg aaggtaccat tagctagttt
     1501 aagtaagatg atgacttttt tacttgcaat agaagctatt gaaaataaag aagtaaaaga
     1561 aactgataag gtaaaaatag acaaatctat tgcatcagta aaagggtcaa gttataaact
     1621 taaagatggt gaagaagtgc cacttataga gcttatgaga ggtcttatga ttgtatcagg
     1681 aaatgatgcg gcaattgcta tagcaaaaca tgtttgtaaa acgaaagaag catttgtaga
     1741 tagaatgaat aaaaaagcaa aagaaatagg aatgagtaat acacattttc taaatccaaa
     1801 tggtcttcca atatacgatt taagcaaccc caaaaaacca gcaaaagaaa atatctctac
     1861 tgcaaaagat atagctattc ttggaaaata catgtttgac cattatgaaa aacaagtaac
     1921 agctataact gatatggaga catatacaaa ttcagataga agttttgaaa aaagcaatac
     1981 aaatgcatta ttaagaatta taccagaagt agatggtata aaaactggat atactggcaa
     2041 tgctggatat tgcttatcat tttctatggt tgtaaataaa aatgataaaa atgagaaaaa
     2101 acgtagagta ataggggttg tattaggtac taaccataaa aataaaagaa cctctgcttc
     2161 attagctcta ctgaaatatg gaaaagaaaa tttcaatatg aaaaaagtaa ttgataaaga
     2221 tagctttatt ggtaagaaat atataaaagg aacgaaagag ttagaggtta cattaaaagc
     2281 aaaagatgaa ctttatacaa tattaaaaga tgatgaaagt ataaagtcag aagttaagat
     2341 taaaaatata gagtaccctg ttaaaaaagg tgatactatg ggtattataa aatattatac
     2401 taattcaggt gacttacttg gaagtgtaga tatagtaagt aataatagta tagataacgt
     2461 atcattaaaa acaaaattaa aaatgaaatt tgcaaattaa attcaaaaaa tggtataaaa
     2521 tacacaaaaa aatataaaaa tagaaaatat ggaaaaaata ttgttatttc ttcacctctg
     2581 tgatatacta ctagaggtga aaaaataaaa taaatcacac acagcattgg gatccttaaa
     2641 aggtgcctat tacatggttt tttagggaga agatcaagct ggaggtataa aaccaggagg
     2701 taagatatgt cagtaatatc aatgaaacaa ttattagaag caggtgttca ctttggtcac
     2761 caaacaagaa gatggaaccc taaaatggct aagtatatat tcacagagag aaatggaata
     2821 tatataatcg acttacaaaa aacagttaaa aaagttgaag aagcttacaa gttcactaaa
     2881 gaagtagctg aaacaggaaa gccaatctta ttcgtaggaa ctaaaaaaca agctcaagat
     2941 gcgataaaag atgaagctga aagatgtgga atgtactttg ttaatgaaag atggttaggt
     3001 ggaatgttaa caaaccacaa aactataaaa acaagaataa ataaattaag agaattagaa
     3061 aaaatggaag aagaaggcgt atttaatgtt cttcctaaaa aagaagttat aaaattaaga
     3121 gctgaaaaag aaaaactaga aaaatactta ggtggaataa aagacatgcc tgaattacca
     3181 ggtgcaatgt tcgtagttga cccaagaaaa gaaaacatag ctattcaaga agctcataga
     3241 ttaggtatac cagtagttgg tatagttgat actaactgtg acccagaaca attagacttt
     3301 gctataccag gaaatgatga cgctataaga gccgtaaaat taataactgg tgctatggca
     3361 acagctgtaa ttgaaggtag acaaggtgct gaggaagaag tagctgaaga ccaagaataa
     3421 taatttgaca attaaatggt aagggtcgcc cttaccattt ataatatgga atagaataag
     3481 taggaggaaa cttaaaaaat ggctaatata actgctcaaa tggtaaaaga gttaagagaa
     3541 agtacaggcg caggaatgat ggactgcaag aaagctttac aagaagctga aggaaacatg
     3601 gaaaaagcag tagatttatt aagagaaaaa ggattatcaa aagctgctaa aaaagctggt
     3661 agagttgctg ctgaaggtct agttgcaata gaaatgaatg atgataatac agttgcttct
     3721 atggtagaag ttaactcaga gacagatttc gttgctaaaa acgaagactt caaagtgttc
     3781 gttaaagatg ctgcttgtat ggcattagct actgataaag aagatatagc ttctttatta
     3841 ggtgaaactc ataaagaagg aataacttta caagaagttt taaataatag agttgcaaaa
     3901 ataggtgaga aattagactt tagaagattt gctaaagttg taactaatgg acaagttgct
     3961 ggatacattc acggtggtgg aaaaataggt gttcttgttg aaatggaaac agaagctaga
     4021 gatgctaaag ttttagaatt aggtaaagat gtagctatgc aagttgctgc tatgaatcct
     4081 aagtatgttt caagagatga agtagatgct gaatacatag cacatgaaac agaagtatta
     4141 actcaacaag ctttaaatga aggaaaacca gctaatatag ttgaaaaaat ggttaaagga
     4201 agattagaaa aagaattaaa agaggtttgt ttattagagc aaactttcgt taaaaatcca
     4261 gatataactg ttaaacaatt agttgctgat gttgctaagg cagtaggttc tgatataaaa
     4321 gttgttaaag ttgtgagatt cgaagttggt gaaggtatac aaaagagaga agaaaacttt
     4381 gcagaagaag ttgctaagca acttaagtaa taaccaaaga gagaacacgc aggtgttctc
     4441 ttttttaaaa aatagaaaaa attggatatg aaggattgaa ataggaggtt tctgatggat
     4501 aaaccaatgt acaaaagggt attgttaaaa ttaagtggag aagcattagc aggagaaaga
     4561 ggttttggta taaataatga tgtagttaat gatattgcta ttgctataaa aaaaatacaa
     4621 gaaataggtg ttgaagtagc tgtagtagta ggtggaggaa acttctggag aggcagaacc
     4681 agtgaaggaa tggacagaac tactgctgac tatataggaa tgttagctac agttatgaat
     4741 gcaatggcgc tacaggatgc actagaaaat atagatgttg ctacaagagt tcaaactgct
     4801 atagacatga gacaaattgc agaaccatat ataagaagaa gagcagttag acacttagaa
     4861 aaggaaagag ttgttatatt tggtgcagga acaggaaacc cttactttac aacagataca
     4921 actgctgctt tacgtgctgc tgagatggaa gcagaagtta tattgttagc taaaaatgtt
     4981 gatgcagtat atgacaagga tccaaaagta catgcagatg ctaagaaatt tacagaatta
     5041 agttacatgg aagttataca gaaagaatta aaagtaatgg attctacagc tacatcttta
     5101 tgtatggata ataaaattcc aataaaggta tttgagctta caacagagaa tataataaga
     5161 gcagtaaaag gcgaaaatat aggaactact gtaaaataat gaagaatgaa ggaggagtaa
     5221 aattatgaaa ctggaaatac ataagcaatt agaagagaaa atgaatggga caattgatgc
     5281 tttaaagttt gaatttggaa ctataagagc aggaagagct aatgcacaaa tgctagacaa
     5341 aataagagtt gattattatg gaactccaac tccaataaat caaataggtg ctatatctgt
     5401 accagaacca agaatattaa tgatatctcc ttgggataaa tctgctatgc atgaaataga
     5461 aaaagcgata gctaactcag acttaggatt aaatccatca aatgatggtg aggttataag
     5521 actttctgta cctgctttaa cagaagaaag aagaaaagaa ttagctaaaa aagctagtaa
     5581 agctgcagaa gaatttaaag ttagaataag aaatgaaaga agagacgcca atgagaaaat
     5641 taaaaagatg gaaaaaggcg gagaattaac tgaagatgaa ttaaaaaaag cacaagatga
     5701 agttcaaaaa atgactgata aatttataaa agaaatagat acattattat ctaaaaaaga
     5761 aaaggacata atggaggtat aatattgaaa aaatattata accttttagg tcttcatgta
     5821 gatgaggtaa aacaatattt tgatgagcaa aatataaatt ataccgtaaa atctattgaa
     5881 ggtaaaaagg ataaagaaaa acttgtagtt cctagagtta taaaaatatc tgagataggt
     5941 gatagtgtag aactgataat cacatacttt tctgactcct tagtttaagg agtcagaaca
     6001 atattggagg caaaatatga ataacaacat tatatatgat atagacttaa ataacatacc
     6061 tactcatatt gctattataa tggatggaaa tggaagatgg gcaaaagcta gatttcttcc
     6121 aagaactgca ggacataagg ctggagtaga aactataaga gatatagtaa aagaatgctc
     6181 aaaattaggt gttaagcact taacattata tgcattttca acagaaaact ggaaaagacc
     6241 gaaacttgaa gttgacacac ttatgaactt attatctaca tacttaagaa atgaaatagc
     6301 tgaacttcac caaaacaatg taaaagtaac tgctatagga gatataagtg ctttaccaaa
     6361 aacttgtata agagaattaa actcagctaa ggaactgaca aaaaacaata caggagttaa
     6421 cttaaattta gcattaaact acggaagtag agcagatata aaaaatgctc ttatagatat
     6481 agtaaaaaat tgtgaaagtg gtaaaataga tataaacaat attgatgaag atattataaa
     6541 aaactattta agcacaaagt ctataccaga tccagacttg gtaataagaa caagtggtga
     6601 acaaagattg agtaactttt tactttggga agttgcttat tctgagtttt actttactga
     6661 tatacattgg cctgatttta aaaaagaaga actacagaag gctatatacg tatatcaaag
     6721 aagggataga agatttggag gacttaagta aggagattat tttatgctta caagaattat
     6781 tgcttcacta gctctggttc cattatttct atttgttgta tatggaggta ttcctctata
     6841 tatagctgag acagcaatag tttatattgc attgcatgag ttttataagg catttaaaat
     6901 aaaagatgta catccaatat tcattatagg atatttattt tctatttatt tagctgtaaa
     6961 aaatattttt aatttaccat tagaatatac ttatgcagta atttttattt tatttttagc
     7021 aagtataatt tatatgttga tgggcaaaaa caatgttata gatgtttcga ttacgttttt
     7081 aggagttttt tatataggag tatttttaga ttttataatt ataactataa atggatttga
     7141 aaaaggtagt atatatgtat ggcttatatt tgtgatatct tttatgacag atatatttgc
     7201 atattttagt ggttatttac ttggaaaaca taagctaatt cctaaagtta gtcctaaaaa
     7261 gactatagaa ggagctattg gaggaataat aggaagtaca ctttgttgta ttttatttgg
     7321 atatttattt ggcattgatt tgcttcaatt agcgataata ggaagtatag gaagtgtaat
     7381 agctcaatta ggagatttat ttgcatcttc tataaagaga tatgtaggga taaaggatta
     7441 tggaaagata atacctggtc atgggggtat attagataga tttgatagtg ttatattagt
     7501 tgcacctttt gtatatagtg cgattaaatt ttttattaga taaagattgg ttggatttaa
     7561 aagagcaatc tataattgct agtaatgaaa atagctatgt aacttttaaa tgcagagata
     7621 ttctgcagtt attagttaca tggctatttt cattgttcaa aaatttagaa gaccttgggg
     7681 aattgtataa gaataggtat tactttataa tagtatacta tatt
//
LOCUS       DACULQ010000167         7719 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.168, whole genome shotgun sequence.
ACCESSION   DACULQ010000167 DACULQ010000000
VERSION     DACULQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.168"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(204..2483)
                     /locus_tag="JA154_15325"
     CDS             complement(204..2483)
                     /locus_tag="JA154_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HAT7997199.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2754..4208)
                     /locus_tag="JA154_15330"
     CDS             complement(2754..4208)
                     /locus_tag="JA154_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAT7997200.1"
                     /translation="MKLNDIIQGLDIINIKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4372..5241
                     /locus_tag="JA154_15335"
     CDS             4372..5241
                     /locus_tag="JA154_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7997201.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKGIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5454..7526)
                     /locus_tag="JA154_15340"
     CDS             complement(5454..7526)
                     /locus_tag="JA154_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT7997202.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVSTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT         2613 a         1289 c          826 g         2991 t
ORIGIN      
        1 ctataatccc atatactctt acctcaaaaa cattcaaatg taaaaaatac tacatattaa
       61 taaactttgc ccaataattc ctaaaataat atcctaacaa tgatttatgt ttttataata
      121 gtatattttt acataaaaaa gtgaacattg attaacaaag ttcacttttt tataaatttt
      181 tatgttataa ttcacaattt tatttaattt gtttcttttc tatttaaact atatctatct
      241 aatattaatt tttcatattc ttctattggc attggtctag caaataaaaa tccttgggcc
      301 atatcacaac ctataaacct taaaaactct gcttgctcta cagtttcaac accttctgaa
      361 acaactttta tatcaagttg ctttgccatt tgaacaatac ttgaaactac aatttgactc
      421 ttttttgcat ttcccactgt tataaagaat tgcctatcta atttcaatac atcaattggc
      481 atatctttaa gcatatttaa agaagaataa ccagaaccaa aatcatccat agaaatttta
      541 aatccaacag atttcaaagt ttgcattatt ttaaataata tttttgtatt gtcaaatatt
      601 gcactttctg tcaattcaat ctcaattaaa tttggttcaa tttcatattc ttttgttatt
      661 ctttcaagct cagaaataaa gcctggattc tcaagatgta ctcttgataa gtttattgat
      721 ataggaaatg tggggaaatt ttcattttcc caacgtttaa aatttttaca aacttgggta
      781 aatacaaaca tatccaattc tgttataaag ccatttttct caaaaatagg aataaataca
      841 cctggactta ttagaccttt tttaggatgt tgccaacgta caagtgcttc agcacctaca
      901 attttagtag ttgataaatc aaccttaggc tgtaaataaa ctttaaattg ttcatttttt
      961 aaagcatcat gcatttcttg ttcaatatca cgttcttcta tgagagtttc ttttaaattt
     1021 tcagcataaa tctcataaac ttttaaatat tttcttttta ctgtagtttt tgcaatcata
     1081 gctttgtcaa gacaggtaga aatatttgtt tctccctctt caacaaaata tacacctatg
     1141 gatggaatta aattgagttc tactcccaaa cttgttttta tcattgacaa ttttttacgt
     1201 ataagttcac atatttctat aattgactct ctattatttt tcttttcaaa tattatagca
     1261 aaattgtcat ttgaaattct tgaaaataca gattgctctt taaatatatt cttaattatc
     1321 tctgctattt cctttaatac tctatctcct tcttcatacc caaacctatc atttactagc
     1381 ttaaacttat ctatatcaaa ataaattaaa actagattct ttttctcatg agaggataaa
     1441 aattttcccc cttcaagatt aaatttattt gaattgccaa tacctgtaac gttatcttta
     1501 aaagcaaatt cttccaattt ttccttactc tttttatgta taaatagtat ataaattgtt
     1561 aatgccgtaa agattataat caagactaca catgctgata atgtaagttt tataatagca
     1621 tatgatttct cagatatagc agttttagga acttcagaaa aaagatacca atcattgatt
     1681 cctattgggg cataacttaa ataatatcct tttccaaaca tactataact taatgaacct
     1741 gatttaaagt tttttatatt tgacttcata ttgttgatac tagattttga tattgaatca
     1801 cttctgccaa tgtaagaaaa taaattttct ttagtagagt tggcgttttt actgcttgga
     1861 tttaaaacaa tatcaccatt acttctagtt atataagaat atccttttcc attaaatgta
     1921 ggtacagaaa gtattttttc atacaacttc gtactatgtg tggcaaatat aactccttta
     1981 acctcattat ctttataaat tggtacagaa tatacattaa tattactatt ggtattttta
     2041 tcatcttcta ttacatcttt taatttgcct gttataacca cttctccttg cattgatttt
     2101 aagaaataat ctcttttaga gaagtcttct atataaccat ctgtagtgta tgctgtacca
     2161 tttggtaaaa taactcccat tcttttaaat gaactattaa tagcttgtct ttttagtata
     2221 gaaaggatat tatctacttc aaaagactct tctccttcaa taaatattga aattgactct
     2281 agtaacttta tatctccatt aatttgtttt tttaacacat tgacactttg atttgctatt
     2341 tctgaaagat attgattcgt ttcttcacct aatgtcttat tcaaccttga accataaatc
     2401 aaacaggaaa ttgtaattac taagatactt atgacagcaa ttatggacaa accataccta
     2461 ttattttttg catcacttaa cattatgctt cctgcctttc tcgaatctat gtatgtataa
     2521 tcattatctt atgacaatta ttgtaaacat tttataataa tacttatttt tccatattta
     2581 ctgtaattat acaaaattat aaactttatt acaacaataa attaattaat atagatatta
     2641 gtgtttatat tattgcatta tctattttac aattatttac atataaaaaa atgtaatatc
     2701 aaagtgaagc ttgatattac atttttgttc ttgtattttg tttaattttt atgctacatc
     2761 ttacttaaac actcatttgc aacttcttta tcatcaaaat gatgcttaac tttacctata
     2821 atttgataag tttcatgtcc cttacctgtt ataattacaa catcatcctt ttttgccata
     2881 cttatagctt ctgctatagc ttgttcccta tcaacaacaa catggtaatt ttcttttttc
     2941 ttgtcaattc cttcaagtat atcttttatt attgcctctg gttcttcagt tcttggatta
     3001 tcagaagtta taatacataa atcagaatat ttttgtccaa ttgctcccat caaaggtctc
     3061 ttttcagtat ctctatctcc accacaccca aacacagaaa ttatttttcc ttctgcaaat
     3121 tgttgagttg tctttagtac attttcaagt gcgtcaggag tatgagcata gtctacaatt
     3181 acgcttatac ctttatcatt tggaactgtt tcaaaccttc ctgatactcc attagaaagt
     3241 ctaaggcctt ctttatatat tggtttaggt atgccaagta catagcaagc agctataact
     3301 gctaaagtat tatatactgt aaacattcct ggaactggta taaatattac ttcttcatat
     3361 gaaggtgtta ttaatctata agatactcca tccgaatctg attttatatc tctagccata
     3421 aaatcagcct tagtatcaac accataagtg taacaaggta catttatacc ttttatattt
     3481 tcatatatct tttttccacc ttcatcatct atgtttatta tatttgccat agtagtctta
     3541 aaaaataatt tttcttttgc ttttctataa tcctctaaat ctttatgaaa atctaaatgg
     3601 tctggtgtta aatttgtaaa tatgcctagt ttaaagtctg tttcatcaac tctatttaat
     3661 gccaatgaat gtgatgaaac ttccatcgca cagtagtcac atccattatc caacatatta
     3721 ttaaaatgat attgtaaatc tatactttct ggagttgttg aattggaaac aatgtctttt
     3781 tctccatcaa aaatttttat agttcctata agtccaactt tattcttatt taaagttaat
     3841 atctcattta gtatagtagt tatactagtt tttccattag tacctgtaac tcctataacg
     3901 ttaaattttt gagatggatg attgtaaaaa ttatcagcta cttttgccat atcttttctt
     3961 gtatctttta cctttataaa tgtacatcct tttatttcta catcttcttc aacaagaaat
     4021 gcacttgctc ccttttctat ggcatctttt atatacttat gaccatctga cacaaatcct
     4081 tttatacata taaataatgt accttttgta acctttcttg aatcatattg tacattatta
     4141 atatctatat ttaattcacc cttgatattg attatatcca acccctgtat aatatcattt
     4201 aatttcataa atgctttcct ccctttaaac tctttctgct aaaattacac tacatataat
     4261 attatattaa gaatttttta ctatgtgaat ggttaaaaat gcttgaaaca ttgcaaaaaa
     4321 tgctctataa ttttctataa tataactgag gaaaggtctt ctataaaaac tatggataat
     4381 aaaatcgggt acttaaacga taactttaaa atatttaata ttagagataa aaaaggtata
     4441 caatttgaat accataatca tgattttaat aagataatta tatttataaa tggaaatgta
     4501 acttattttg ttgatggtat tccctataaa ctaaagcctt gggatatact ctttatcagc
     4561 aataatgaaa tacataaacc agttatagat tcaaatgtct tttatgagag aattgcaata
     4621 tggattagcc cagaatttat gaaaaaaaat agtgatgcta gtagtgactt agaaaaatgt
     4681 tttaaaatag ctacagagaa taaatgcaat agattgcgtt taaatataca tgatattgaa
     4741 tctattaaga gttttattga gcaaataaat atttccgaat ccaatactga atttggaaat
     4801 gaaatattga gaaatgcatt atttatacaa cttatgatat tcttaaacag aattttccta
     4861 aaaaatgaaa atatggaatc aacagaagat gttttttatg atgaaactat taagaaagta
     4921 ttaaactata taaattcaaa tatagatgat aagctttcaa tagatagcat atcgtctgaa
     4981 ttttttataa gtaagtatta tcttatgaga aaatttaaat ctcatactgg aacgtctata
     5041 cacaattata tagttcaaaa aagattaatt ttggcaaagt cactaataac caatggtcac
     5101 cttatgagtg aagtttgtaa taaatgtgga ttcaacgatt attctagttt tgtaagggca
     5161 tttaaaaaaa actacggggt atctcctaaa aattatataa acaataaaaa tcctttggat
     5221 attactcttt tagatgaata gataatttat gtaaatttta ttttaataaa acttgaagta
     5281 ttagtgtatt tatcttacta atacctcaag ttttttaatg ttttaaaata tatcaaaata
     5341 taatgatata tatttctatt acttaaaata tatattaaat aaattgcatg ttaactgaac
     5401 ctatttatat atttattaaa tcccacataa cttttagtta cactatcata tagctatcct
     5461 attttttcaa tatcatctct taatatactt actctattct cctcttttgc tgtgcttact
     5521 ccttttttaa tgcttaattt ctgattttgt tctaaattcg taaatactac aggacttatt
     5581 gttgatagac catttttttc aatatattct aagtccattt taactaattt ttctcccgct
     5641 tttacttcat cccctacatt cacaaatact tcaaatccat ttccttctaa atttacagtt
     5701 tctatcccta aatgtataag tatttctact ccactttttt gggattttat tattattgca
     5761 tgcttagttg gaaatataac tccaatagtt ccatcaactg gtgaataaat caatccatca
     5821 ctagatttaa ttgcaaatcc atcccccatc agtttacttg aaaatacaga atctggcact
     5881 tcttctatat ctaatatctc tccatttgtt aatgaatcaa tcttttctac taaagcattt
     5941 tccatatttg caacttccat attttttttc actttatttt tatcaacatt tttagtaaca
     6001 gtactttcaa cctttgtttt tccctctatt atatctttta tatcagactt tattgaatcc
     6061 gattttggtc caaatatagc ttgaacattg ttgccaataa ccataacacc tgctgctcca
     6121 agtgatttca atttttcttt atctacttta tttatatcat ttacaaccaa tctaagtctt
     6181 gttatacacg catctaggct ctttaaattg tctttatttc ctaaagcaat caaaatctct
     6241 gatgctaatt cattcttaga catttcaatg acttcagcgt tagtttctga ctcatcttct
     6301 cttcctggtg tctttaaatt aaattttctt atcataaatc taaatacact atagtataat
     6361 actgcaaaac caatacctac caagatagcc aagtaccatg gagttctatt aggaagaaca
     6421 ccaaataata agaaatctat taatcctcct gaaaatgtga atccaatttt aactcctaat
     6481 aattgcataa ttgcgtatga taaacctgaa aataaacaat gtattgcaaa taatactggt
     6541 gcaacaaata aaaacgcaaa ctctattggc tcggttattc ctgttaaaaa agaggttaat
     6601 gctgctgatg ccattatacc tgctacaaat tttttctttt ctggctttgc ttcatgcacc
     6661 atagcaagag ctgctgctgg aagaccaaac atcataaatg gaaatgcacc tgtcataaaa
     6721 gtacctgctg tgaaatttac tccatcttta agttgagcaa agaatatctt ttggtcaccc
     6781 attacaagct caccagcttt atttacatat tctccaaatt ggtaccaaaa tggattatgg
     6841 aaaacatgat gcaaaccaaa tggtattaat agtctttcca aagaaccttc cataaatgct
     6901 gctagtgttt gatttgcttc tataacattt tttgagaaac taagtaatcc ctcttgtatt
     6961 ggaggccaaa taaaagacaa tataattcct attgctatag caactaatga agtaacgatt
     7021 ggtacaaatc ttttacctgc aaaaaaacct agatactgag gtaattcaat tttataaaac
     7081 tttttataaa gataagcagc cactatccct ataataatgc caccaaatac ccctgtttga
     7141 attgttggta ttccaacaat atttgcatac atagaattgt ctaccatatc ttctgtaaca
     7201 cctgccataa cacccataga tgtattgaga attaaaaaac ctactatggc tgccaatgct
     7261 gcaacacctt ctccttcagt taaaccaaca gctaccccaa ctgcaaatat aagtggtaag
     7321 ttattaaata taatttgacc tgaactttcc attatactta ttataagttg aaatgtagga
     7381 ttcgccaaag ctggaaaatg agccaacaat gcgtctcctg ctaaagcatt actcacgcca
     7441 agtaatatac ctgctgcagg aagtaatgca actggtagca ttagagattt tcctactttt
     7501 tgtaaaacac caaatacttt tttcatttac ttttctctcc ttattatttt atattttttt
     7561 aaataaaaaa gacttaagtt tataaaaagt tacattagta tacttaattc taaaattaaa
     7621 cttatgttaa tataaataaa cctatattaa cctaatttca gaattaaaaa taaactatta
     7681 taattttttt ataaacataa gtcttgcctg cattatcag
//
LOCUS       DACULQ010000168         7712 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.169, whole genome shotgun sequence.
ACCESSION   DACULQ010000168 DACULQ010000000
VERSION     DACULQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.169"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..821
                     /locus_tag="JA154_15345"
     CDS             <1..821
                     /locus_tag="JA154_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997203.1"
                     /translation="SVDIIRESDFKKLRQYQNNNYEELTSKESVYYVSDSYKKIFFKD
                     FEIEKINLHIKDANEYDINLFKVQKSILEPEINSQATFDLIVVKDDVFNQLKLHGNSR
                     LIRLIDIKNEKKELDLTLKLKNIVSENMKFTYPFNFTSSIESYDNLIKLSGLMLFIGI
                     FLSVVFLLCTGSIILFKQLSNIYDDKERYIMLIKLGANNKDIEKIISKQLKVIFLMPL
                     VVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIYFIGYFIYYFLTLKYALNMIKE"
     gene            1046..2659
                     /locus_tag="JA154_15350"
     CDS             1046..2659
                     /locus_tag="JA154_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAT7997204.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2792..3643)
                     /locus_tag="JA154_15355"
     CDS             complement(2792..3643)
                     /locus_tag="JA154_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAT7997205.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNGLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            4269..5162
                     /locus_tag="JA154_15360"
     CDS             4269..5162
                     /locus_tag="JA154_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding oxidoreductase"
                     /protein_id="HAT7997206.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            5162..6556
                     /locus_tag="JA154_15365"
     CDS             5162..6556
                     /locus_tag="JA154_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088038.1"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
                     /protein_id="HAT7997207.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            6922..>7712
                     /locus_tag="JA154_15370"
     CDS             6922..>7712
                     /locus_tag="JA154_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HAT7997208.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWT"
BASE COUNT         3011 a          865 c         1351 g         2485 t
ORIGIN      
        1 cgtctgtaga tattattaga gaaagtgatt ttaaaaaatt aagacaatat cagaataaca
       61 attatgaaga attaacttca aaggaatctg tgtactatgt ttctgattca tacaaaaaaa
      121 tattttttaa agactttgag attgaaaaaa taaacttgca tattaaagat gcaaatgagt
      181 atgatataaa tctgtttaaa gtacaaaaaa gcatcttgga gccagaaata aattcacaag
      241 ctacgtttga tttaattgta gtaaaagatg acgtatttaa tcagttaaaa ttacatggaa
      301 attctagatt aataagatta attgatataa aaaatgaaaa aaaagaactg gatttgactt
      361 taaaattaaa aaatatagta agtgaaaata tgaaatttac ttatcctttt aattttacat
      421 caagtattga aagttatgat aacttgataa aattaagtgg tctaatgctg tttataggca
      481 tatttttgtc tgtagtattt cttttatgta caggtagtat aatcttattt aaacaattat
      541 caaatatata tgatgataag gaaagatata ttatgttaat aaaacttgga gcaaataata
      601 aagatattga gaaaataata tcaaaacagt taaaagttat atttttaatg ccattagttg
      661 taggtacagt tcataattta tttgctatgt ctatagcaca aaagtttata ccaaggtcat
      721 tacttgtacc aattatcata actctagtca tttattttat aggatatttt atatattatt
      781 ttttaacttt aaaatatgct ctaaacatga taaaagaata aattattttt aagttaaaag
      841 aattagatat atgttttgaa taaaataact tatttaagtg cagatagaaa atactagaat
      901 tgagtttaag atatttattt aaagataaaa ttaaagttag gaaaaagtct atattttata
      961 tcattttgta tcagttacat taattgatgt tatataataa atctaagcag aatatcaaat
     1021 aaaaaatgac gaggagctga aatttatgaa gtttaatgcc taccaacata aatactcatc
     1081 aataagaaat gtcgtgtatg ctaaaaatgg agcagttgca acatcaacac cattagcatc
     1141 tcaagctgga cttgaaatac taaaaaaggg tggaaatgct gttgatgcag cagttgcaac
     1201 agccgccaca ttagcagtag tagaacctac aagcaatggg attggaggag atgcatatgc
     1261 ccttgtttgg atagaggaag aaaaacgatt gtatggttta aattctagtg gctttgctcc
     1321 agaaaatatg gaattaaaaa attataataa tatgaaagaa atgccaaaat atgggtttgg
     1381 agcagtaact gtacctggaa taccagcagc ttggtcagaa ttaaataaaa aatatggtaa
     1441 acttagctta atggaatgct tatctccagc gataaactat gcaagagagg gatacgtagt
     1501 atctcctaat gtagctaagg tatggaaaaa atcatatgaa ctctatgaaa aagaacttat
     1561 tggagaagag ttcaaaccat ggtttgatac attttctaaa gatggtaaag ctccagaggc
     1621 tggagatata tttatatgtg aagaacaggc aagtacatta gaagagatag caaatactca
     1681 ggctgaaagc ttttatagag gaagacttgc tgataaaata gatgaatatt ctaaaaaatt
     1741 taatggggct ataagaaaga gtgacttaga gtgtttttat ccaacatggg tagaacctat
     1801 aagtacagaa tataagggat ataaaatatt tgaaatccca ccaaatggtc atggaataac
     1861 agttttaatg gcacttaaca tattaaaaga attggaatta gaaggaaata tagaaaatgt
     1921 agaagacata cataaaatta tagagagttt aaaattagct tttgcagatt ctaaaacata
     1981 tgtaactgat atagagcata tgaaggtaaa gattcaggaa ttactaagcc aagagtatgc
     2041 taaaaaaaga agtttattga ttgacaataa agaggctttg tatccaactg caggagagcc
     2101 atattgtggt ggtacagtat acctttgtac tgcagataag gatggaaata tggtttcata
     2161 tatacagagc aattacataa attttggttc aggaattgtg ataccaagaa ctgggattgc
     2221 tttacatagt agaggaaata attttaattt agaccctaaa caccacaatg tagtaaaacc
     2281 atttaaaaaa ccttatcata ctataatacc tggattttta ggtaaagaag ataaagcaat
     2341 aggtccattt ggtgtaatgg gagcttttat gcaaccacaa ggacatattc aagtgttaac
     2401 aaatatgatt gattttggat taaatccaca agaggcgcta gatgctccaa gatggcagtg
     2461 gataaaagga aaggaaatag aagttgaacc agagatgcca aagcacataa tagattcatt
     2521 aattgaaaag ggtcatgaaa taaaagtaat tcatgatact gtcgacatgg gacgaggtca
     2581 aataatattt aaaacagaac aggaaagtta tatttgtggg acggaatcaa gatgtgatgg
     2641 tcatattgca gtttattaat attatcaact aaaatatata taataaattt aaagagtata
     2701 tgaaataatt agtaaaggcg aaagtctaca atgactgacg cctttacaaa atttaagcta
     2761 tcatatgctg attttatata gattatgagc cttattcttg accagttttg atttcattgt
     2821 catcataact aatccagtca ctaaagctac caatataaat cttgtgaggt atatccagtt
     2881 ctctaagagc aagactgtta acacaagcag atatccctga accacaagat aaaattactt
     2941 catcataatt atttaaatct ttaaagaatt tttttaaaaa ttctatatct ttaagtgagc
     3001 cattttcaaa gtcacttttt attaaatcct tacaaaaata attttttgca cttggtatat
     3061 gaccagcttt tgagtaagca ggttcaacta aaccctggta tctctcatta gcacggcaat
     3121 ctattattac agtatctttt ttgtataatt tagacttaac atattccatt ggaacaacta
     3181 aaccattgtt tatatttggt ctaatttcct tacctgtaca acttggtata ttcactttct
     3241 cttccaattc tccaccttcc tttacgaaag atgttattcc cccatccagt acatagacat
     3301 ttttaagccc caaatgtttt aattggaaaa ataatctgca agctccattt aaatctccat
     3361 catcataagt tacaatcgta gtgtcgttat caacacccat tttttctaat ttttctttta
     3421 gtattaaagg gtcttgtaaa ggatttttac caccatgttc ttttgtagga gatgataaat
     3481 ccttgtctat atctaagata aagctgcctt tgatgtgtcc ttttttataa gaatcaatac
     3541 cataagttct attgataaga tcaaatctgc aatctattac aactagatta tcattttttt
     3601 ctagttttga tataagttct tttgctgaaa taatgttttt caaagaatgt acctcctgtg
     3661 tttatattat ctttatttta atttatatta attattaact ttataaacta atattattat
     3721 aatttattac ataagtaaat aaatctttag aaattgtaaa aattttatat aaaaattcaa
     3781 ataattaatg aatattgaaa attttagata tataataaaa taagagtata ttttatacaa
     3841 tatgaaagct ggtattgcaa tagattaaag ccttatacac gtttagtttt taaagaaatt
     3901 agtgttatta tgaaaaccta agttaattta ttaaataaat aattgaataa aaaaatttta
     3961 ttatgtattt ataaaatttt tgttacaaat ttggcataat atagtatact ttaagtgtgt
     4021 attaggttaa atctataaca aattagacgt atgatataga cttagtgttt attggcattt
     4081 acaagaaatt tgttaatatc aactatttgt agtttgggca gttataatta ttataggggg
     4141 gatgtccaat acaaaacaat caaccaagaa ctaatattat attataaaga gttatagttt
     4201 tgtgtgttta tgtttttaat taaatttaaa gtgggaaaaa tatatttgtt agaatggaga
     4261 atagcgagat gagtaataaa atagttagta agagacaatt aacagatagt atttatttaa
     4321 tggaaataga ggcacctaga gttgcaaaat cttctcaacc aggtcaattt ataataatta
     4381 aaaatgatga aaaaggagag aggattcctc ttacaatagc tgactatgat agagaaaaag
     4441 gaacagtaac tattgtattc caaacagttg gtgcttcaac taaaaagtta gcaatgtttg
     4501 aagaaaacga ttttgtaatg gattttgtag ggccattagg acaagcaagt gaatttatac
     4561 atgaagatat agaagaatta agaaataaaa agatattatt tgtagctggt ggagttggtt
     4621 ctgctccagt ttatccacaa gttaaatggt ttaaagagca tggattagat gtagatgtaa
     4681 taattggtgc aagaactaaa gaattaatta tattagaaga tgatatgaaa aaagtagcta
     4741 aaaatgtata tgtatcaaca gatgatggta catatggatt taatggaaga gttacagacc
     4801 tattaaaaga tttagttgat aatcaaggta aaaaatacga ccaagcaata gtaattggac
     4861 caatgataat gatgaaattt atgtgccaat taactaaaga actaaatata cctactatag
     4921 taagtttaaa tacaataatg atagatggaa caggtatgtg tggtggttgt agagttagtg
     4981 ttggaaatga aactaagttt gcatgtgtag atggtcctga atttgatggt catttagttg
     5041 attttgatca agcaatgaga agacagtcta tgtacaagac tcaagaggga agagctatgc
     5101 taaaacttga agaaggcgat agtcatcatc atagtaattg tggatgtgga ggtaacaaat
     5161 aatggatgct aaaaaagtaa aagtaccagt tagagaacaa gaacctgctg tgagagcaac
     5221 aaattttgat gaagtatgct tagggtataa taaagaagaa gctatggcag aagctaatag
     5281 atgtttggct tgtaaaaaac caaaatgtgt gggtggatgt ccagtaggaa tcgacatacc
     5341 agggtttata acaaaaatta aagaagatga tatagaaggt gctgctaaag taatagctaa
     5401 gagcagttca ttaccagctg tttgtggtag agtatgccca caagaaagtc aatgtgaagg
     5461 tgtatgtata cttggaataa aatcagatgc tgtatcaata ggtaaattag aaagatttgt
     5521 agcagattgg tcaaaagaaa atgacataaa tttatctgat acagagccta agaaaaatca
     5581 aaaagtagca gtaataggaa gtggtccagc aggtctagct tgtgctggtg atttagctaa
     5641 aaaaggctat gatgtaacaa tctttgaagc aatgcatgag ccaggtggag ttttaactta
     5701 cggaatacct gagtttagac ttccaaaaca agctgttgtt caacctgaaa tagacaatat
     5761 aagaaaatta ggtgtaaaaa tagaaactaa tgtaattgtt ggtaaaacaa ttacagtaga
     5821 tgaacttata gaagatgaag gatttgaagc tatattcata ggttcaggag ctggtcttcc
     5881 aatgtttatg aatataccag gggaaaatgc aaatggagta ttttctgcaa atgagttttt
     5941 aactagagta aacttaatga aggcttatag agatgattat gatacaccta ttagttctgg
     6001 taaaaaagtt gctgtagtag gtggtggaaa tgttgctatg gacgctgcta gaactgcatt
     6061 aagattaggt tcagaatcat atatagttta tagaagaagt gaaaaagagc ttccagcaag
     6121 agctgaagaa gttcatcatg caaaagaaga aggcattata tttaatacat taacaaatcc
     6181 taaggaaatt ttagtagatg aaaatggata tgttaaaggt atggtttgta taagaatgga
     6241 attaggagag ccagatgatt ctggaagaag aagacctata gaaatagaag gttctgaatt
     6301 tgtacttgat gtagacacag ttataatgtc acttggaaca agcccaaatc cactaatatc
     6361 ttctacaaca aagagtcttg atataaataa gaagagatgc ttaataacag atgaaaatgg
     6421 tcagacttct aaagagggtg tatttgcagg tggagatgct gttactggag cagcaacagt
     6481 aatatctgca atgggtgctg gtaaaacagc agcagcatca atagatgaat atttaaaagc
     6541 aaaagttaat gcttaatttg taaattaaag agagggtgtc tcaaaatgaa ctttttagtt
     6601 catgaggcac tctttttgta caaaatcaaa tatgttttaa tcatttcaac aatgaaaatt
     6661 tcattcctgt agaaagtcta acttttaggt cttagtacaa tctctatttt gatgtagcca
     6721 cttttttaga tggtttaata atttatcagt tattattgta atatttgata ctaagtaaca
     6781 taactttgtc taactatgat aaaatagtta tacaataaag gtcttgtaag gagtgaataa
     6841 tgtgtggcta ataaaatcag atttgtcaaa tctaacatta aataatagaa aaataaattg
     6901 tgtaattaat ggtattttaa tatgaaaaaa agagaaaaag tatttgatgg gctagttttt
     6961 aagcatatat taatcttatt aatactatta gttttgattg atttaacaag taccatcata
     7021 tatgcaagta actctatgga aattacttct agaaatatga ttgaggcatc aaaaagagaa
     7081 cttgaaaact atttagaagt aaacatatct ttattaaaag ctcttagtca agatgataga
     7141 ttttctaatg gtgaaacatc actaattgaa aagggaaaat tattaagtcc atatcaaaag
     7201 gaatataatc tttttatgat tggtataaca gatacaaaag gaaatgcttc aagtacatat
     7261 agggaaaatg ttaactcaat aaaagataag cctagttttg agaaagcaat taaaacaaag
     7321 caagtagtag tttcagatat tgaagtcagt aatgtaacag gtgacaaagt atttatcata
     7381 tatgtaccaa ttgtaaaaaa taatgagatg attgggacca tttttgcttc tttttatttt
     7441 caagatgtaa ataatttaat aagtagaagc aattttgatg attctattaa atttctaatg
     7501 atagataaag attatactat tatttctcat ccaaacaaaa aatatgtaaa tgataaaagt
     7561 aagatgcttg atttagaagg gaatattata ggaaccacta aaagtgaaat cttaaaaaat
     7621 ataaataaaa aatgtcaagg tgatttttta tcttgggata attggagatt atataatgta
     7681 aaatatacga acataaagtg gactaattgg ac
//
LOCUS       DACULQ010000169         7555 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.170, whole genome shotgun sequence.
ACCESSION   DACULQ010000169 DACULQ010000000
VERSION     DACULQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.170"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            52..690
                     /locus_tag="JA154_15375"
     CDS             52..690
                     /locus_tag="JA154_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7997209.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            822..1484
                     /locus_tag="JA154_15380"
     CDS             822..1484
                     /locus_tag="JA154_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7997210.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            1484..2665
                     /locus_tag="JA154_15385"
     CDS             1484..2665
                     /locus_tag="JA154_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7997211.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(2846..5506)
                     /locus_tag="JA154_15390"
     CDS             complement(2846..5506)
                     /locus_tag="JA154_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997212.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5503..6186)
                     /locus_tag="JA154_15395"
     CDS             complement(5503..6186)
                     /locus_tag="JA154_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997213.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(6530..7483)
                     /locus_tag="JA154_15400"
     CDS             complement(6530..7483)
                     /locus_tag="JA154_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein transpeptidase
                     domain-containing protein"
                     /protein_id="HAT7997214.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT         2617 a         1174 c          970 g         2794 t
ORIGIN      
        1 cataagtata ccatcagtat acttttttat caatagaaag gaataattta tatgtcagaa
       61 ttatcaaaaa gagataaaga aaaaatacaa agagaaaatg agattattga taaagcagaa
      121 aaattatttt gtcttaatgg atttgataat acaactatga atgaattagc aaaagaagtt
      181 gaatatacta aacgcaccat atacaagtac ttttcatgta aagaagattt attctttgct
      241 gttgtcttaa gaggatataa aagactttgg gataatgtta aaatagaaag tgcaaaaggc
      301 aaaacagggt ttgaaaaaat aaaactttca tactttgctt ttcataaatt ttactctgta
      361 gaaccttctc tattatgcct tatgggaatg attggtattg taaactctga aaaatcggat
      421 actgatatgc tgtttaaaga aaagtttttt tcttttaata aatttatgtt tgatgaaata
      481 caaggaatgt ttgaaattgg aaaaaatgat agaagtatta ggcatgatat tgaaatacct
      541 actcttatgt attcatcaat atttacactt acaggatttt ttaatttact atctgtaaca
      601 ggaaaatcat acctgaacaa ctttaatata gatgaggaaa aatttattga aacaactctt
      661 gctcttttaa tagattcaat taaagcataa acctaagttt ataaattaaa gtttatatac
      721 ttaaatgata attttttcta tctctaatat aaaaaatatt aaacataaaa ctaataactt
      781 agttaatgga ataactaaat tactgaaagg attaaaagta aatggctaaa atacttcttt
      841 tagaagatga tgatacaatt acatttggaa taaaaacttc ccttgaaaag aaaaatcaca
      901 ctgtatgttg ttataatacg attgctgaag ctaaaaaaaa ttttgataaa actattgatt
      961 taattctgct tgatttaaat ctacctgatg gtattggata cgagttttgc caatatgtta
     1021 aaagtataga agatactcca gtgatatttt tgacagtccg tgatgatgaa aaagacatta
     1081 tacgtggatt agatatgggt gcagatgact atgtggttaa accgtttttg ctctcagtac
     1141 ttcagtccag aattaatgca gttcttagaa gaactcattc aaatactaac aatactatta
     1201 catgtgaaaa tataagcttg aataaggttg aagtcaaagc atatttagat aatgttgaag
     1261 tacctttgac agcaggagaa tataaacttc ttctaagtct tatggaaaat aaaaatagag
     1321 ctatgactcg tacaaagtta ctagaaatgc tctgggatgt agatggtaat ttcgtaaatg
     1381 ataacactct tactgttaca ataaaacgtc ttagagaaaa attaaaaaat ccaccttgta
     1441 taaaaacgtt aagaggcata ggatatagaa tggaggacag ctagtgaaaa ctccaagaaa
     1501 tatcaacagt acagttgttt ttataatatt ctgcttaatt gtaacttttt tattgagtgt
     1561 aattctaaca actataactt cttttaatga atacaatgta ctgtctaagg ctcttggtgc
     1621 atcatatgag tacaatccta agtctacaga aagtatacta aaatcactga aaaattctag
     1681 tgataaggat tttaaaacag gaaatgaaat tttatcaaaa catggatatt ctaaaagtac
     1741 attttggagt aaaaactttt tttattttct agtgatatcg tttggtataa tttcaacaat
     1801 ggctacagtt atatatataa tcaaacatag aacaaataag caaaaactga gtagaataaa
     1861 caatcttact caatacttag aagaagtgaa tttaggaaaa gagtctgtac ttttacgatg
     1921 tgaagatgaa ttttctcacc ttgaagatga gatatataaa acagttgggg aacttcgttg
     1981 ttcaaaagaa tttgctttaa aagaaagaca aactctttca gataatcttg cagatattgc
     2041 tcatcaactt aagactccaa ttacttctat gtcacttatg gcacaattac tttcagaaaa
     2101 ttgtccagat gaagaaatag attatattaa cagacttaca aatcaaatct caagacttga
     2161 gagactagtt tcatctttac ttacattatc aaaattggat gcaagtactt taatatttga
     2221 aagcaaactt atagatgtac attctcttct tacttatgca attgagccta tagaaggttc
     2281 tttaagagaa aaacatcaga catttactat cgaggcttct ccttcggtag aatttgaaat
     2341 cgatattaat tggactttag aagctctttt aaatattctt aaaaattgta gtgagcatat
     2401 agaagatggt ggatatatag ttgcttatta tagtcaaaat cctctatatg ttgagattat
     2461 catagaagat aatggtaagg ggtttgctca ggaagaactt ccatatattt ttaatagatt
     2521 ttatcgtggt aaaaatgcta gtaaggacag tattggtata gggcttgctc tatcaaagtc
     2581 tattatagaa cgtcaaaatg gtactctaca tgcagaaaat tgccataatg gtggtgcaag
     2641 attcataatt aaattttaca cataaaatgc tggattatag gtaaaatacc tataatccag
     2701 ctatatctat attctatgtt atctattgtt ttagtaatat acttctattt acatttaagg
     2761 ttttattaaa caaacaactt aactatccct atacttgtaa gtagtcttaa atcgaaacct
     2821 ttaaataagt tcaaatagat tttgtttata ttgtttcatt ttttatagat tctactatat
     2881 tttcattctt caactgcttg tatcctgtca tatatgatgc tattacaata aaaagtatca
     2941 atatagtaaa acctattatt gtagatatag gtgcaaatgg taaatactct ataaaatata
     3001 tttcatttat acgtagaaaa gcatacacta ttcctatttg cacaggaata ctaagtagta
     3061 atggtgtaat tcctaagaat aaaccttcca aaataagcat cttttttatg ccttctggag
     3121 ataatccaac agaacgcaac atagcaaact cttgtcgtct acttctaata tttcctgaca
     3181 ctgtagcaag tacatttgat aatccaatca tagccaataa acctgtaata aagcctacta
     3241 ttaaattcat ggtctttatt ccactagcct tttgtttttc actttctaaa acatcctcaa
     3301 tactataatc tcctgaacca tagtctttat tataaatgga ctctaatttt tctcttactc
     3361 taggaatgtc ctcacgatta tttactttaa atactcctat cagcctattt gaacgtaacc
     3421 tacttacttc atcataattt ttagatattt cctgtaccat agacattgga actatttgca
     3481 ttaatgtata ctttgaacta gtatctacta ttttaggaag tgtgcttgta atagctccca
     3541 ctttcatgtt gtaagtataa tcccctttat cttcgtcata tactttttca tttaatttaa
     3601 tgttatctcc tattgaaaga tttaaataat ctatatatac tttatttcta cgtgtactac
     3661 gattaatatc ttcctcctta ttatatacaa ttgaggttgg attttttgta ttataaaata
     3721 actttgggtc tacagaaatt tgcctacaat attctgtaaa actcttatca tcaagcccaa
     3781 ttatatttga aattatacga aacttgccat cacgttctat aggagagtat tttttagatg
     3841 aaactatact tttaaatcct ccatttttcg caagttcttt tgatgcatct ttttcagtca
     3901 gccaagtgga agcagacatt ttttgataat atagtgaact ttttacacct tctgtattat
     3961 ctaatttttc aatgaaagca gactctatag gttcactatt ttccaaataa actgctatat
     4021 ctctttggtc cttataatta tcacttccat aaacagcttt tgctccttct tgatttgcaa
     4081 ttatatgcaa aaaacatgta agtaataaaa aagataatgt taaagatatt gttgcagtac
     4141 gataagattt tttacgagca cgtagtgcgt ttgatgcaag ttctccttct attccaaata
     4201 tctttcctaa tgaatattta tgcgactttt tgattttaac actatctcct tgtcgtattg
     4261 cttctatagg agatatcttt gatacttttc gtgctggtat aagtgcagaa atccagactg
     4321 ttactattgt aagtaaaact gctggcagaa aagcaggaat accataagta aataccactt
     4381 ctggaacatc agtatgataa ttaactgaat taacataaac tataagccta ttacacaata
     4441 accatcctaa aaataaacct attggtaatg gtataattgt cagtaaaaag ccttcaaaca
     4501 ctacagacct tttaatttgt tttggtgatg caccaacact agataatatc cctaactgag
     4561 taagtctagc atttgcagac agtgaaaacg cgttgtaaat caccattaca aacacggcta
     4621 ctgtaaaaat tgctattgta agatacataa taggagttga aaattttgat atactactca
     4681 tagatgcttt ctgttctggt gaaaagacta acatctttaa aagataatta ctattgtatt
     4741 taagattgta ttttccatat tcattagtct tccatcccaa tgatttagca agttttggaa
     4801 gttccttata cgtgtctctt atatttttaa atcgcaagta cacagttata ctatcattag
     4861 gttttatact tgttttatca agatatccaa gagctgtata tgcaggtatt actgatgaag
     4921 tagaaacatc tacctttcct actattgtca atgtagcctc tgaaattttt ttaaaacttt
     4981 cccctttcga ttttggggct atgggttcaa gaggctttcc atttaagatt ctatatccta
     5041 ttgggagagt aattttatca ccaattttta cacttggatt attctcaaaa tattgttttg
     5101 ataatgcaat ttcattagca ttatttggtc gacgaccctc tattatagta tccttttctg
     5161 gcatagaatt ccaatactct gatgtagcat ttctataaat tatataatct cttttctcat
     5221 cattaatttt gcctacatac cattgtcctt ttaccattac atcatcaact gaagaaaaat
     5281 tttcaattaa tggtaagtca cttccataag tatagtcaaa caattcacca tgccagttac
     5341 cttctttctc aattgaaagt cttataaaat ctgtccacat ggtatacaac agtccacaca
     5401 aagaagacat catggttgta gtaagtaata tagctaacat tatagctata ctactacgtt
     5461 tattgcgtcg tacaaagtct aaggtgtatt cttgaagaat attcatttct tcaccacctt
     5521 ttcagagaca atcactccat cttccatagt tacaatacga tttgcctcta gtgctatttt
     5581 ctcatcatga gtaataagaa gtattgtctg attaaatcgt ttatttgata agtttaacaa
     5641 gtctacaatt tcttctgaat tttttctatc aagattacca gttggctcat ctgctaataa
     5701 aatagctggt ctatagatta aacttctagc tatagatacc ctttgttgtt gtccacctga
     5761 aagttgactt ggaagatggt ttaatctatc agataatccc aatatagata caatctcaga
     5821 gaatctatcc tcatccacct ttcttttatc aagtaacatg ggtaataata tattttttct
     5881 aacatcaagg gtaggtataa gattataaaa ttgataaata agacctactt ttctacgtct
     5941 aaaaactgca agagcttctt cctttaaact tgatatttct gtatcttcaa cataaatctt
     6001 tccagatgta ggtctatcta ctcccccaag cagatgtaac aatgtagatt tgccagaacc
     6061 agatgctcct acaatggata caaaatcacc tttttgaact gataaattta cattattaag
     6121 ggctgtcact ctggtttgat ttgaaccata tatttttgtg agattttcac attttaatat
     6181 ttccatatac aatcatctcc tttatggtta aattttagca acctaaaatg acattatagt
     6241 gacatgaata caaatataaa ttactattaa aaatattgtt tagtttttgc acattattta
     6301 aaattataat ataaaacaaa aactatgcaa tatattttat ttatactttt aatatattac
     6361 atagtcttta aaaggtatta actttaaatc tataggtatt caatttaata atcaatagca
     6421 agtattttat gtactaaagg agttttgctc tatatagact caatttagta aaattattta
     6481 ataaatatta cagactatag gtaaataata tttacaaata aaattaagtt tattccctta
     6541 cactataata tttcttaatt atattaattg caatttcctt tacctttggc cctgtaattt
     6601 ctttattgga atcatctgat ttaatagcaa aataataggt gtcaccttca cgctctagca
     6661 taccaacaaa ccatgcatcc acacgtttat ttttttcatc aaatcctgtt ccagtttttc
     6721 catatacatt tatgttttta tcatttatat ctattttcat aatatctctt aaaatattaa
     6781 tatgctcttt tttaaagttt gtatctccct caaatatttt tgccattgtt tgcacttgtt
     6841 cttttggaga tatttgtaat gaagattcta accaaaatcc atttaaatgt ccctttccat
     6901 tttttaaatc gccttcccat tcgctaatat cgcaattccc atactttaaa tcatctaaaa
     6961 tgttttggac agatttagca tcaactttat ctatcaattt tttgtaatac caaacacaag
     7021 actccttaaa tgcctcttct aaactaagat ttttattcca atttttattt ggatactctg
     7081 taccatcata tcccataact gattcttttg agtttattac ccctttttct aaaccaatta
     7141 aagttgaaac tattttaaaa gtagaacatg gtgaccttct tgtttcaatt aattctttgt
     7201 tatatatatt atattcttta tttttagtat tataaaaaat agctcctcca ctaatacctt
     7261 caaaacaatc actatagtca accatattaa ttttttcttt tttgttttta tcattgtttt
     7321 caattttgga tttatttaat tcagacttat ttaattcaga ttttatataa ttcttatcag
     7381 ttttttgatt tatatcgtta tgttttttcc ctacataata cattaccata actacaccaa
     7441 ccaataagat agctatccaa ataaaattct tttttctttt cataatttat atcatctccc
     7501 atatttaaat ttataaccta ctattaaaat agtagacatt atttgtaata tactc
//
LOCUS       DACULQ010000170         7552 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.171, whole genome shotgun sequence.
ACCESSION   DACULQ010000170 DACULQ010000000
VERSION     DACULQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.171"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            299..1492
                     /gene="aksA"
                     /locus_tag="JA154_15405"
     CDS             299..1492
                     /gene="aksA"
                     /locus_tag="JA154_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087309.1"
                     /note="in Methanococcus jannaschii this protein catalyzes
                     the condensation of alpha-ketoglutarate and acetyl-CoA to
                     form trans-homoaconitate; functions in alphaketosuberate
                     synthesis which is a precursor in coenzyme B and biotin
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoaconitate hydratase"
                     /protein_id="HAT7997215.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1517..3442
                     /locus_tag="JA154_15410"
     CDS             1517..3442
                     /locus_tag="JA154_15410"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087310.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HAT7997216.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREEAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3464..4459
                     /locus_tag="JA154_15415"
     CDS             3464..4459
                     /locus_tag="JA154_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent isocitrate dehydrogenase"
                     /protein_id="HAT7997217.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4809..5258
                     /locus_tag="JA154_15420"
     CDS             4809..5258
                     /locus_tag="JA154_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7997218.1"
                     /translation="MIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKGQFTFLTRI
                     CENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLTPTDKSLEV
                     YELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5292..5786
                     /locus_tag="JA154_15425"
     CDS             5292..5786
                     /locus_tag="JA154_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7997219.1"
                     /translation="MIRKANMNDLESIMQIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5883..6404
                     /locus_tag="JA154_15430"
     CDS             5883..6404
                     /locus_tag="JA154_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAT7997220.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            6593..6904
                     /locus_tag="JA154_15435"
     CDS             6593..6904
                     /locus_tag="JA154_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT7997221.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6932..>7552
                     /locus_tag="JA154_15440"
     CDS             6932..>7552
                     /locus_tag="JA154_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT7997222.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVK"
BASE COUNT         3039 a          752 c         1467 g         2294 t
ORIGIN      
        1 ttttataggt ttatttttaa aggttttttg aaaataaatc tatttaaaaa ttaaattaaa
       61 atgcaagaaa aacataataa ctttcatttt taagaagttt tcacaaaaaa gaaaaaatat
      121 agtgcaataa atgatacaaa gtaatataat agattacaag atatgaaaag ctaaaaaaat
      181 aaattgcaaa tgaaattaaa atgaataaaa aataaaaaaa taatcataag ttagaggtat
      241 caatgaaaaa taagacttaa tgttttaaaa ttaaaagtat actaaggggg gcattaatat
      301 gtgcgttata tcaaaagata gagcaaagga aataaaaata gtagatacta ctttaagaga
      361 tggagagcaa acagctgggg ttgtatttgc aaatagagaa aaaataatga ttgctgaaat
      421 gttaagtgac ctaggtgttg accaaattga agttggtata cctactatgg gtggagatga
      481 aaaaaatgta ataaagcaca tatgtagtag aaatttaaaa tcagatataa tggcttggaa
      541 tagagcagta ataaaagatg ttgaagaatc tatttcttgt ggtgttgatg cagtagctat
      601 atcaatatca gtatctgaca ttcatataga aaataaattg agaacttcta gaggctgggt
      661 tttagaaaat atggctaaaa cagttgaatt tgcaaagaaa aatggattat atgtttcagt
      721 aaatggagaa gatgcttcaa gagcagatat agacttctta actgaattta taaatgttgg
      781 aaaacaagca ggggcagata gatttagata ctgtgataca gtaggagtta tgaatccttt
      841 ttctataaag aatgcaatag aaactcttta tgaaagaact aattttgata tagaaatgca
      901 tacacataat gactttggga tggcaactgc aaatgcactt gcaggaatag cagcaggagc
      961 aaattatgtt ggtgttacag taaatggatt aggtgaaaga gcaggaaatg cagcattaga
     1021 agaagtttta atggcactta agtgtgttta taaatgtgat ttaaataata tagatactag
     1081 aaaatttaga ggaatatgtg agtatgtagc acaggcatca ggaagaatac ttcctacatg
     1141 gaaaccagta gttggggata atatgtttat acatgaatct ggtatacatg cagatggagc
     1201 tttaaaagac ccacataact atgaaccatt tgacccaagt gaagtaaatc ttgaaagaaa
     1261 aatagtaata ggtaaacatt caggaagagc tgcagttgta aataagttga gtgaatatga
     1321 aatgtatatt tctccagaaa acgctaccaa gctattaaat gctataagag ctacatctat
     1381 aagattaaaa agaagtttaa tggataaaga aattcttcaa ttatattgtg atatactagc
     1441 acatgagaaa ggcacaacag aagaagaagc agttagaggt tcatatattt aagatataat
     1501 tgtttaggag gaaaatatgg gagataatat agtttataaa ataattaaaa aacatatagt
     1561 agatggagaa gctgttgctg gaagttcaat tggtataaaa atagaccaaa ctttaacaca
     1621 agattctact ggaacaatga catatcttca actagaagct atgggaatag ataaagtaaa
     1681 gactaaaaga agtgtggcat ttgtagacca taatatgtta caacaaggat ttgaaaatgc
     1741 agacgaccat aagtatatac aaacagtggc agataaatat ggagtttatt tctctaagcc
     1801 aggaaatggt atttgtcatc aagtattttt agaaagattt tcaacaccag gagatacact
     1861 tttaggttct gatagccaca cacctactgc tggaggtgtg ggcatgatgg ctattggagc
     1921 tggtggatta gatgttgctt tagccatggc aggaggagca tactatataa aagcaccaaa
     1981 agtctgtaag gtgaatctag ttggaaaatt aaataatatg gtatcatcaa aagatataat
     2041 attagaagtg ttaagaaaac aaactgtaaa aggtggagta ggtaaagttt atgaatatgg
     2101 tggagaaggt gtcaaaagcc tatctgttcc acaaagagct acaataacaa atatgggggc
     2161 tgaacttgga gcaactactt caatcttccc aagtgatgaa aaaacactag aattttttaa
     2221 gagtcaagga agagaagagg catggataga gctaaaacca gatgcagatg cagtttatga
     2281 tgaagagatt actataaact tggatgaatt aaaaccatta gctgcaaaac ctcatagccc
     2341 agacaatgta gatgaagttg aaaatatagg caagattaaa atagaccaag tggcaatagg
     2401 aagttgtaca aattcttctt atgaagactt aatgaaagta gcacaaatcc tgaaaggaaa
     2461 taaagtacat aaggatgtga gtttggtaat agcaccaggt tcaagacaag ttatggagat
     2521 gattgctaga aatggggctt tggcagatat aataagtgct ggagctagaa tactagaaaa
     2581 ttcttgtggt ccatgtattg gtatgggtca atctccaggt actgactctg tgtctttaag
     2641 aactttcaat agaaatttct atggtagaag tgggactttg tcagcacaag tatatttagt
     2701 aagtccagaa gtggcagcag taagtgcaat caagggagtg cttacagacc caagagaatt
     2761 tgatattaaa tttacaaatt tagatgtaaa tgaatttttg atagatgata gcatgataat
     2821 taaacctgct gatgtaggaa gtgatgtaga agtagttaga ggacctaata taaaaccttt
     2881 ccctcttaat acagagctaa gtcagtctat aggtggaaaa gttatattaa agacagaaga
     2941 taatataact actgaccata taatgccatc aaatgctaag ttacttcctt ttagaagtaa
     3001 tataccgtat cttgcaaact attgtttcaa tactgtagat acagaatttc ctcagagagc
     3061 aaaagacaat aatggtggat ttatagttgg tggagataat tatggtcaag gttcaagtag
     3121 agaacatgca gctttagcac cattatatct aggtgttaaa ggggtaatag ttaagagctt
     3181 tgcaagaatc cacaaggcta acttgataaa tagtggtata attccaatgg agttttgtga
     3241 tgagaaagat tatgaaaata tatctctttt agataatctg gagataccaa atattttaga
     3301 caatctaggt agtggaattt tagaagttaa gaatacaact aaaggaactt cttttaaggt
     3361 caaagtagaa ttgtcagcaa aagaagttga tgtattaaaa gctggtggaa aattaaacta
     3421 cactaaaaat caagctaatt aatatgaaaa gagggaatat tgaatgtata aagtgacact
     3481 tataccagga gatggaatag gaccagaagt tgcgaaagct atgaaaaagg tagtagaagc
     3541 tactggagta gaaatagaat gggaagaagt taatgcagga gaggcagtta ttgaagagta
     3601 tggtactcca cttcctgaat atattataga tagtataaaa aagaacaaaa tagctataaa
     3661 aggcccaatt actactcctg taggtaaagg atttaggagt gtaaatgtaa ctttaagaca
     3721 ggcattagat ttgtatgtca atttaagacc tattaaaagt tttaagggga taaaatcaag
     3781 atatgaagat gttgatttag tggttgtaag agaaaataca gaagatttat atgctggaat
     3841 agaacataaa ataggtgatt atgcagctga aagtataaag ataataacaa gaagtgcaag
     3901 tgaaagaatc gttgattttg catgtaacta tgttaaagat aataagagaa aaaaagttac
     3961 agctatacat aaggcaaata taatgaaaat gtctgatgga ttgtttttag atgtgtttag
     4021 agaagttgca tcaaaacatg gtgtagaata tgatgatttg attgtagatg cagcagctat
     4081 gaacttagtt ttaaatccag aaaattatga tgtaatggta atgccaaatc tttatggaga
     4141 tatactatca gatttaggtg ctgggcttgt tggaggttta ggtataattc caagtgcaaa
     4201 tattggtaaa gattgtgcga tatttgaagc tgttcatgga agtgctccac aaatagcagg
     4261 tcaaaataag gctaatccaa cagcacttat tcaatcatca gtaatgatgc ttagatactt
     4321 aggtgaatat gaaaatgcac aaaaaataga aacagcttta gaaaaagtat ttcttgaagg
     4381 aagtaaattg actgtagatt tgggtggctc agcttctact acagagtttg cggatgaggt
     4441 gtgtaagtat attgtataga ttttatttag ggaaaaagta aaatatagat tgaaggaaag
     4501 aggctttctc aaaatagaga taagcctttt ttattggaat gataaaatat atttgatttt
     4561 atacaaaaga gagttcctca taaacgaaaa agttaatttt gggatatcca ttttaatttt
     4621 aaaattaatt gattaatatg caaagaaaga ttaaataaat ttattatttt taattgaact
     4681 ttcaaccaaa atatggtaaa atttaaaaaa gttgaacttt caactaaaaa tgaatatatt
     4741 gattatgtga aattttataa aacagaaact atttgacttt agccatagta gtttcagtgg
     4801 aggtgtgttt gattaagact ttagatagta atatattaag agaagtaggt actttatcta
     4861 gagcagtaaa ttcaataaat gatattaagt acaaggaatt aaagttacaa aaaggacaat
     4921 ttacattttt gacaagaatc tgtgaaaatc ctggtataaa tcttgtggaa ttatcaaata
     4981 tgttaaaggt agacaaggct acaacaacca aggctataca aaaattaata aaggcaggat
     5041 atgtagataa aaagcaggat aagtttgata aaagaggata taatttaact ccaactgata
     5101 aatctttgga agtttatgag ttgataattg aagaagagaa tagaagtata gagatatgtt
     5161 ttgataattt tacagatgaa gaaaaacaag tggtaactaa gttattagaa aaaatgagca
     5221 aaaacgtaga aaatgaatgg tttaaagtaa agagatagta aataaataca ttaacttaag
     5281 gagatatttt tatgattaga aaagcgaata tgaatgattt agaaagcatt atgcaaatta
     5341 taaaatctac agtggaagaa atgaagactt ataataatac acaatgggat gaaaactatc
     5401 cattagaaaa agactttgtt tcagatatta agaaacaaga tttatatatt tatgaagtgg
     5461 atggagaagt agcagggttt atatgtctta attatgaaga gccagaagaa tatttaggct
     5521 taaattggtc atctaataaa aaagcaatgg taatacatag aatggctgta aatccaaact
     5581 ttagaaagac aggaatagca tctaagttag ttgattttgc tgaaaaatta gctgtagaaa
     5641 acaatgtatc atacttaaag agcgatacat attcaataaa ctcaaaaatg aatttattac
     5701 ttacaaagtg tggttttata aaaatcggag aaatgagttt tttaggaaag gagaaatctt
     5761 tctactgtta tgataaaata ttataaagag catttataat acaagggata aataatagat
     5821 tttcagcatt agtaagggca taatataacc tagtgtatgc tattatgaaa gggatgattt
     5881 atatgattgg atttctaaag aaaagaagaa gtattagaaa atacaaggat gttgaagtag
     5941 aaaaagaaaa attagataaa atattaaaag ctgctctttt agctccatca tcaaaggggc
     6001 ttagaacatg ggaatttata gtggtagatg ataaagaaaa gctaattaat ttatctcaat
     6061 gtagaactaa aggtggtgga tttttcttaa aaaatgcacc tttagctatt gtaattattg
     6121 cagataaaga aaaaaatgat gtttggatag aggatgcaag tatagctgct tcatacatac
     6181 aacttcaagc tcatgaacta ggtttaggtt cttgttggat acaagtaaga aatagaatgc
     6241 atgatgataa tatagaagct gataagtaca ttagagaaga attaaaagta cctagcaagt
     6301 acagtgtaga atgtataatt tctataggat atcctgatga agaaaagaaa gcatacggtg
     6361 actctgattt agactataaa aaggttcatt ttaacaactt ttagtatata atatgttgta
     6421 ttaagacatt atattttttt ctattgttaa tatatactta aaattgtaaa aatatatctg
     6481 aattttacta acttatctag gggttatgta caaggatttt aaagataaat atagaaatta
     6541 atgtttataa tcgatacata acttgagtaa caaaatttaa ggaggttaaa gtatgggtat
     6601 atttaaaaca aaaatggatg aggactggaa agtaaactac attaaagagt ttaatgaaat
     6661 gagagattct tatgagagta aacttcaaaa aaaacagttt gaggtagata gtttgaaatc
     6721 agaattggat agactaagaa gctataaaaa tagccttaag ccaaaagaaa aacaaattac
     6781 tgatgatgat ataaataata taaagagttt aagaagagat ggcttaagct ataaagaaat
     6841 ttcaaatcaa acaagttgga gcaaagcaac tgtaagtagg gtattaaatg gactttacga
     6901 ttagcatata catggggtga tttaaattgg gatgaagtat agttacaaaa aatgcataat
     6961 agattctatt ttattgatgt ttatagttca aatattaagg atgattttga attatgtact
     7021 tctaagtcaa tttgaattta ctcttgaaaa ttttaatatt ataaacttaa tttcttttac
     7081 attagttggt ttatcgttaa ttttattttt aaaagataat tctttgtata acaaggttag
     7141 aaatagaaaa atcacagaag cttttgaaga aaataaaaac aatatactta ttgaaaagtg
     7201 taagctgata ctttttgttg tggttttatc tttagcaata attgtaactt attgtactaa
     7261 gggatatgta ctttttaatg taactatgat gactttgtct gtattgatag tcccaatatt
     7321 tgaagagtta ttttttagag aatatatatg gaactatcta agtaatttta taaagtcaaa
     7381 gagtaaaatt atctgtataa catctatact atctggaatt tataatatag gatacataga
     7441 tgttataaga aattatgtaa tattgtataa caattcaagc tacacctttg aagttattat
     7501 ttcaaaaatt atgataggta ctgtttttgg tattgtactt ggattagtta ag
//
LOCUS       DACULQ010000171         7523 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.172, whole genome shotgun sequence.
ACCESSION   DACULQ010000171 DACULQ010000000
VERSION     DACULQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.172"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..711
                     /locus_tag="JA154_15445"
     CDS             <1..711
                     /locus_tag="JA154_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997223.1"
                     /translation="NFFGNIYYMKSEFDKILEKELNDEIRIEVINSLYKQIQEQRNNL
                     DLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNLLSDLSYNISSYLNEVEKSLDN
                     IIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRLNHLVENMYPYYVDETSREMFK
                     LFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLNNSTKERRKDIIEKIYNSVDYG
                     TNKLDENMFFTGGIKYVK"
     gene            701..1021
                     /locus_tag="JA154_15450"
     CDS             701..1021
                     /locus_tag="JA154_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997224.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            1213..2088
                     /locus_tag="JA154_15455"
     CDS             1213..2088
                     /locus_tag="JA154_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HAT7997225.1"
                     /translation="MQMTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIE
                     EAIESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHY
                     IRQKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGV
                     QKIAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSIL
                     EKQTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENL
                     KDDLMDYMRKKVSEK"
     gene            2112..3125
                     /locus_tag="JA154_15460"
     CDS             2112..3125
                     /locus_tag="JA154_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HAT7997226.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            3237..5171
                     /locus_tag="JA154_15465"
     CDS             3237..5171
                     /locus_tag="JA154_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HAT7997227.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            5350..6189
                     /locus_tag="JA154_15470"
     CDS             5350..6189
                     /locus_tag="JA154_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HAT7997228.1"
                     /translation="MSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEYKYAVSQGD
                     YVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDELPLFLSRL
                     ECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARIVIESRDDV
                     SNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIADILCNPLS
                     RARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIVEPVGPWLE
                     KMR"
     gene            complement(6397..7278)
                     /locus_tag="JA154_15475"
     CDS             complement(6397..7278)
                     /locus_tag="JA154_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT7997229.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
BASE COUNT         3030 a          800 c         1362 g         2331 t
ORIGIN      
        1 aatttctttg gaaatattta ctatatgaaa tctgaatttg ataagatttt agaaaaagag
       61 ttaaatgatg aaataagaat agaagttata aattcacttt ataagcaaat acaagaacaa
      121 agaaacaatt tagatttatt gtcagcatat atgccaagaa tattagaaaa tcaagaaata
      181 gatttgtttg aaaagtataa tactaaactt gatgtacaag tgtttaagca gattaaaaat
      241 ctattgtctg atttatcata taatataagc agttatttaa atgaagttga aaaaagttta
      301 gacaatatca tctttgtaat ttcaggagaa gataagaaga aatacaatta tagaatgtta
      361 aaagaaagtg aaagtgcaag tcttgatgta tttttaaaac aagccatatc aagattaaac
      421 catttagtag aaaatatgta tccttattat gtagatgaaa ctagtaggga aatgtttaag
      481 ttatttgagt tattgtggaa aaactataga aatattgatg atgatagtat aaaagaaata
      541 ttattcgaga gaataacaaa tacatatgta tttaaacaat tgacattaaa taatagtaca
      601 aaagaaagaa gaaaagacat aattgaaaaa atatataact ctgttgatta tggaaccaat
      661 aaactagatg aaaatatgtt tttcacagga ggaataaagt atgtcaagta aatacaatgt
      721 ttggactttt aactatgagt tcttaggatt ggactcaggg gatgaaaaaa ataatgaagt
      781 ttattacgag attgatttag atgaaatttt aaatgaaaac tatatagatg aagatataga
      841 tagtggttta gattctgatg gaaatagtat tttaggagaa ttaatatcag aaatggatat
      901 agatgaggat gtcagtgttg atatgacata tcaagaacta gaagttgact tagaagattt
      961 agattcttat ctagatgaag atatagatag tgatataaaa ggcatcttag atgaaattta
     1021 gtacctgtat aagttttgga taaaatataa acaatacaat attaacttat aaaactaaag
     1081 gtagatgaaa tttcatctac cttatattat cttatagcat taaatattgg gataatatag
     1141 agtttgaaga aaaagtataa atatggtata ttaatttggt acaatagaaa atttacgaag
     1201 aagggtgaaa aaatgcagat gacagttaaa aaagcaataa taccagcagc agggctagga
     1261 actagatttt taccagcaac aaaatcacag cccaaagaaa tgcttcctat agtagataaa
     1321 ccaactttac agtatataat agaagaagct atagaatcgg gaattgaaga aattcttata
     1381 ataacaggta gaaataaaaa atctatagaa gatcattttg ataaatcagt tgaattagag
     1441 ttagaactag agcaaaaagg taagacagag atgttagaaa tggtaagaga catatcaaat
     1501 atggtaaata ttcattatat aagacaaaaa gagccaaaag gtcttggaca tgcaatttac
     1561 tgtgcaaaaa gttttatagg tgatgaacct tttgccgtat tattaggtga tgatatagta
     1621 gattcagatg ttccgtgctt aaaacaatta atagatactt ataatgagta taaaacaact
     1681 gtattaggtg ttcaaaagat agctaaagaa gatactaata aatatggtat attggatgta
     1741 aaacatatcg aagatagagt atataaagtt aaagatatgg ttgaaaaacc tgcaattgaa
     1801 gaagctccat ctgatatagc tatacttgga agatatataa taactcctgc aatatttagc
     1861 atattagaga aacagacacc tggtaaaggt ggagagatac aactgacaga tgcacttcaa
     1921 actcttggaa aacaagaggc tatttatgct tataactttg aaggtagaag atatgatgtg
     1981 ggagataaaa ttggtttttt agaggcaaca atagactttg ctctaaagag agaaaattta
     2041 aaagatgatt taatggacta tatgagaaaa aaagtaagtg aaaaataaat attagaaaag
     2101 aggtaattac aatggcagtt ttagtagctg gaggagcagg atatatagga agccatacag
     2161 caatagaact tttagaatca ggttatgaag ttgttatagt tgataactta agtaatagca
     2221 actcaatagt ggttgataga atcaaggaat tatctaaaaa accagtaaaa ttttataata
     2281 ttgatatcag aaataaagat gaaatgcatg ttgtatttaa agaaaataat attgaatcaa
     2341 ttatacactt tgcagcttta aaagcagttg gtgaatcagt agaaaaacca attgaatatt
     2401 acagcaataa tctaataagt accttaaatt tatttgaatt aatgagagaa tatggtgtta
     2461 aaaagtttgt atttagttct tcagctactg tatatggaga cccacatact tgtcctatat
     2521 tagaggattt tccactttct gtaaccaatc catatggaag aactaaactt atgatagagc
     2581 agatgttagt agatatatca aaagcagata agtcattgga tatagctctt ttaagatact
     2641 ttaatccagt cggtgctcat aaaagtggta gaataggtga agaaccaaat ggagttccga
     2701 gcaatctaat gccttatata acaaaaatag cagtaggtaa attgaaggaa ttgagtgttt
     2761 atggtaatga ctatccaaca catgatggaa ctggagttag agactatata catgtattag
     2821 atttagctgc tggtcatgta aaagcattac aaaaattaga agaaaatcca ggacttgttg
     2881 tttataattt aggaactgga aaggggtata gtgttcttga tttagtaaaa gcattttcta
     2941 aagcaagtgg aaaagaaata ccatataaga ttgtaggaag aagggctgga gatgtagcca
     3001 tgtgttatgc agattcttca aaggcagaaa aagagcttgg atggaaagct aagtatgaac
     3061 ttgaagagat gtgtgaagat tcatggagat ggcaaagtat gaatcctaat gggtatgaag
     3121 agtagaatac atacttatat gtataaatgt aacaaatttg taaatataag gtattggtaa
     3181 ttttatgtta taattagaca catgtagtaa tgtttctaaa agagggggaa gttatagtga
     3241 aaaaaagtct aaaaaagtat ttagtactag cactgacttt agttttagta tttgcatgtg
     3301 gcgtaccaga atcaagcgca gccagtaaac atgtgattat agtaaattca agaaaaaata
     3361 ctctaggata ttttgtaaat aataaacttg taaaagaatt tagagttgca acaggtaaaa
     3421 aggggtcaga aacaccaaca gggaaaacaa aagtagttaa caagataaaa aatagaccat
     3481 attataaagg taatatacct ggaggaagtc caagaaatcc attaggagat agatggatgg
     3541 gattagcatt aaaaggaact tatggagata catatggaat acatggaaat aataatgaga
     3601 gttctatagg aaagcatatt tctggtggat gtataagaat gcacaataaa gatgttagat
     3661 ggttatttga ccaagttcct gtaggttcag atgtaattat agattatagt aacgacagct
     3721 atgtaaagat agcggcaaaa tataagataa atctaaatca aacaggatgg aaaacagaaa
     3781 atggtaagaa gtattatgta aaatctgatg gaacatacca aaagaatagt tggctaaaag
     3841 taaatggaaa aatgtattac tttgatgctt caggagtaat gcaaacagga tggaaaacaa
     3901 ttaataacaa aaaatattat ttaggaactg atggagcaag agtatctgga tggaaagtaa
     3961 tagacggaaa aacttattac tttaactcag atggagtaat gcaaacagga tggcaagaaa
     4021 agaatggaaa gaaatactat ctaggatctg atggtttagc agtaacagga tggcaagaaa
     4081 tagatggtaa taagtattac tttgacaaaa caggaataat gcagacagga tggcaacaaa
     4141 tagatggtaa gtcttactat ttagacaaag atggaaagat gctaactggt tctcaaaaaa
     4201 tagatgggaa agattatact tttaatgaag atggaactat aaatcctaca tgggatacaa
     4261 ttataggtgc aaatagattt gatacagcta aaaagataag ttcagttgga aactggaatg
     4321 ctgatagttc agatactgta atattagtaa atggtaatgc aatagctgat ggtataacag
     4381 caactccttt agcaagtagc tatgactcaa caatactact tactaataca gctaatttac
     4441 caactgaaac tgttgaagaa atgaagttat tagcacctaa aacagtaatc ttgataggtg
     4501 gagaaaatgc aataagttct aaattagagc aagaaataaa aactactttc aacacagaaa
     4561 caaagagaat agcaggtcaa gatagatatc aaactgctac aagaatagct gaagaacttg
     4621 gaaatcgaga agaaataaaa actgcatata tggtttctgg aaatggggag gcagatgctt
     4681 tatctgtagc ttctaaagct ggggaagaaa aacaacctat aatattagtc aataaagatg
     4741 gaattacaga agaatcttat aaatggctaa cagaaagaaa attagaaaat gcatacttta
     4801 taggtggacc aagtgcaatt aacgattcag taatagcaaa aatgaatgat ataactactg
     4861 aagatatttc aggaaataga atatatggag atagtagagt agatactaat gccaaagtga
     4921 tagaaaaatt ctatggagat actgatttac aggctgtatt agttagtaaa tcagatgctt
     4981 tagtagatgc tttatcagct ggaccattag ctgtaaaatt acattcacca atagttttaa
     5041 tggataattc aggattatca tcagagcaac aaagagtatt tgcaaataaa aaagttgaaa
     5101 ctccttatca aataggaggc ggagtatctt atatagtaat ggataaatta atggatattc
     5161 ttgcaaaata gtaaaaatat atattgaaaa ataattataa ttgagatgtg aaattcaatc
     5221 aataaaagct tgattgtttt acatctcaat tttttagtat aaaattaagt ttaagttata
     5281 taaaaatctt ggaatatttt taaaactcat ataaaatgaa tatagatgct atcatcatat
     5341 gttaagaggg tgagtaagat tataaagaaa aaatatataa tgtttattga tgaaagtgga
     5401 agagctgata taaatcatga agacccattt acattgacag gagtaatttt tgaatataaa
     5461 tatgcagtaa gtcaaggcga ttatgtatgt tctttgagga aagaaatgga tgcatttaaa
     5521 gagtattgtt ttggaaccag taacatagga atacatttga cagatatatc aagaaaatca
     5581 agagcatttg agaagtttga tgattcacag attaagttat tctatgatga gttgccttta
     5641 tttctatcta gattagagtg tactataata tcagtaacag tagataaagc tagattaaag
     5701 aaatattatg caccttcaaa ggaaccgtat gttgtggctt ttatacatgt tcttcagaat
     5761 ttttattcat ttgtaaataa tgaaactgta gaatctgcac gaatagttat agagtcaagg
     5821 gatgatgtct caaacttaaa agtacaaaag gcgttttttg atgtatttaa taatggaaca
     5881 atatacttag atatagatga agaattaaga gataaggtta agggttttat aattgccaaa
     5941 aaagaagatg ctgactatag agaaggttta gaaatagcag atattttatg taatcctctt
     6001 agcagagcaa gaagagggtt aatagaagct aatccaaagt gtatgcaata tggagaaaaa
     6061 aacaaaatat tcaaatctgt cagaaacaaa atatatactc caacgagtgt gaatgatata
     6121 agaaattggg gatttaaaga agttcctatt gtagagcctg ttggaccatg gcttgaaaaa
     6181 atgcgttaga tacaattgac aaattttaaa aaatctgata taatgtaagt aacctagtta
     6241 ttcaggaact gaatccagtt gctgttgtat aacgataatt actgagactc ggatgccgac
     6301 tgtcagttag gaatctatac ttttaaaagt atagattttt ttattttata aaaagaataa
     6361 aagaggatat ttaaaaataa cccctttatt aataaattat aattgagcgt ttttaatgac
     6421 aaagttttta aagtttttaa ttgcaggagg caaatatcta tcttttataa atgctaagta
     6481 aaagtttctt tcccaacttg ggtgaataat ctgaattact tttacttttg taaagctaag
     6541 catattcata tttgggacta cagcaattcc gaaatttttt gcaactaatc cagcaattac
     6601 ttggtcttcc tctacttcat agactatatt aggctgttga tttatttttt taaacatatc
     6661 atcaattata tatctaagac cacttctctg attaaatact atttgaggat aaggtatggt
     6721 ttcttttaaa tctatagtat cctttgcagc tagtggatga ctatatggta caattagtac
     6781 taaatcttgt ttggcaacag gtataaattc aattcccttt tctttttcaa gtttcgaaca
     6841 aaatgcaata tcatattttc tttctttaag accttgaata atgtcagtag ttacaccagt
     6901 atgaaattta aaatctattg atttagcagg attagatttt aaaaatttgt gcactatgtc
     6961 tgggataaaa tcagtaccaa gtgttctaag aaaagctaag tctatttgtc cttcaccagt
     7021 accagttatt tttaatgatt ttacacttga atctaaaatt tctaatgatt tttccacatc
     7081 ttttaaaaat atttttccat acttagtaag gactatattt ctaccttctt tttcaaatag
     7141 agccacccca agttcatttt ctagtaaaga aatagcatga cttaagcttg gttgtgttat
     7201 agataaattt tctgccgctt ttgtatagtg ttctaaatgt gccaatgtaa caaaatagcg
     7261 taagtgatat aaattcaatt tttgttcatc ctttcatagg tatttttatt tatatatttg
     7321 tagaagttaa cctttaagat atttttacat ttttattata tatgattata gagtaagaat
     7381 aaaagctttt aaaaaaattt atcgtttttt gtctgaatta taagataaat atatttatac
     7441 gacctaatag aaaaatacaa gaattatctt ataaataaaa tctataaaca aaatctatga
     7501 attaatagga aattatcaat ttg
//
LOCUS       DACULQ010000172         7382 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.173, whole genome shotgun sequence.
ACCESSION   DACULQ010000172 DACULQ010000000
VERSION     DACULQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.173"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            105..254
                     /locus_tag="JA154_15480"
     CDS             105..254
                     /locus_tag="JA154_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAT7997230.1"
                     /translation="MFFISYIYTKSPIEFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCV"
     gene            complement(355..3129)
                     /locus_tag="JA154_15485"
     CDS             complement(355..3129)
                     /locus_tag="JA154_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HAT7997231.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(3301..4557)
                     /locus_tag="JA154_15490"
     CDS             complement(3301..4557)
                     /locus_tag="JA154_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAT7997232.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(4629..6131)
                     /locus_tag="JA154_15495"
     CDS             complement(4629..6131)
                     /locus_tag="JA154_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HAT7997233.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(6222..>7382)
                     /locus_tag="JA154_15500"
     CDS             complement(6222..>7382)
                     /locus_tag="JA154_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7997234.1"
                     /translation="YKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLVSLDIEILDLG
                     METGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHACGHDIHTSCLV
                     GCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAIFGLHNHPDIP
                     CGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMGIQTIVSRNIS
                     PLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLENIVCEIARGYQ
                     ATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGEDFSIFMEKAPG
                     FFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKLKNKI"
BASE COUNT         2307 a         1414 c          906 g         2755 t
ORIGIN      
        1 tttgtctgta catattttaa atttatacta tatttatcta tattttataa ccttatgata
       61 cactataact ataagttttt aaatggaaag gaatgaaata aatcatgttt tttatcagtt
      121 atatttacac aaaatcacct attgaatttg atactccttt gcaaaaagag gcatacaaaa
      181 tattacaaaa attagatatt gactttgaat gtgttgacac agatgaagca attacaatgg
      241 aagattgtgt ttagattaat aaaaaattaa atatgaaaat ggtaatataa aacaacatta
      301 aacaaacaaa aaccgcaaag ttacaatata tagtactttg cggtttcata ggaattaatt
      361 tttactatta aagttcatag ttctactatt tttcattgtt ctaagaatta gctttacaaa
      421 ttcatttgac aataggatag tggatgcaaa tagtattatc tttatccaca tatcaaagtc
      481 caaagaaaca gaattaaaga aactctggaa cacttgtgta aatattattt gaattatccc
      541 tgtaactact attatttgca atgccagttt atttttaaag aaatttggta ttgtactatt
      601 taaaccaaac tctctacaat ttaatgcatt aaaaagggca ctgaatgcaa ataaagaaaa
      661 tataacagta ccttgttctt ctgatgtagc tcccaatata ttaaaagctg attgagtaaa
      721 tacaattgta attataagaa tagcatttat aataatattt acaaacatag acctagctat
      781 tattccagaa tgtctattta ttggttttct tttcaataca taatctctaa caggttctaa
      841 tcctaatgct agggctggag gaccatccat tattatattt acccataata attgtattgt
      901 agtaaatggc atatccttac cagtaagttg agatattata gcaattataa atgctactat
      961 atttacagta agctgaaact gtataaatct ttgaaagttc tcatatatac ctcttcccca
     1021 ttttattccc tctactatag tactaaagct atcatcagtc aatattatat ctgctgcatt
     1081 tttagatact tctgttcctg aaatacccat tgcaattcca acatctgctt gactaagtgc
     1141 tggggcatca tttataccat cacctgttac agctacaact tcgccacttt tttgaagtgc
     1201 tgaaactatt ctcatttttg tatctggttt acttcttgca acaatagata ttcctttgat
     1261 ttcttctctc aattctttat ctgtcaatac atctatatat gttgcttcaa ctgccttttt
     1321 cccatcattt aataatccta attcatttcc aatagctgtt gctgtattta tattatctcc
     1381 tgtaagcatt tttacatcta ctcctgcatt aaatgcttta tcaatggaat ctttgacccc
     1441 ttctctcaat gggtccacta ttcctacaaa tccactaaaa actaaattat cttccttcat
     1501 ataacttctt gaatttccta ttaaattcaa ttcaccttct gaagtcattg caacttctgt
     1561 tttagaatta ctcatttttt tataagcaaa tcctaatgtt ctcatagatt taatttgtaa
     1621 cttcttaatc tcatctaaaa tacttttttc tacttttgga gttattggaa caatattttt
     1681 tccttgttgt acataagaac atttttttaa taatacttct ggagcacctt ttgaaagtaa
     1741 tatatctgaa tcctcttgac gaattaatgt tgacatcttc ttttcttctg agctaaatgg
     1801 tatttgtgat atcaaatacg tttgttttct catttcatta tagttcttat cattatgata
     1861 taaaagtaat gcacactctg ttgcgcttcc tatatattta aatgaattat cttctttttc
     1921 tatatctgca gttgaattta ctatacagtt ttgttcaaaa tagctattac tttgatactc
     1981 tcctccactt atatatttac catcaacata tgctacctct accatcattt tgttttgtgt
     2041 taaagtccct gttttgtcgg aacatattac agaaacagaa cctatagttt cacaggcttc
     2101 tttctttgtt actaatgcat ttatctttgc catcttttgc attgttatag ctaaggtaat
     2161 atttatcata gttggcaaac cttctggaac agttgcaact attaatgcaa tacaaaccat
     2221 atatgcattt ttagcaggtt ctaatgattg taagaaagat aatacactgc ttgtatctaa
     2281 aattaacata ccttttctct gcatctgtat aatcataaac atacataata aagtagctat
     2341 tacgcctgat attcccgcta ttttagcacc caacttccct aatttaattt gaagtggagt
     2401 ttgctggtct tcatttgata aattctgtgc tatcttaccc atctctgtct tatcaccagt
     2461 atgagtgaca accattatgc ccctaccata tgctacaaga gtccctccaa ataccatatt
     2521 gacttgcttt gcaggtattg catcctgttc gattatttct gttttagaat aaactacttc
     2581 catatctaat acagcatcag cattcttagc tacatcatca gattctcctg ttagcatatc
     2641 ttctctaagt ttcaaattta tagactgtat aagtcttcca tctgctggta tcatatcccc
     2701 agtttctata tatacaatat ctcctggtac taaatcattt tttgatattt taattatctt
     2761 cccatttcta agcaccttta cttctatatt ttctgttaat tttgacaggg catgagctgc
     2821 ttttttagat tttccttcag ttattatgcc tatagaaatt ccaataaaaa tagcccctaa
     2881 aatacctaat gcgtcatgta attctcccac aaaagaactt attactgctg ctcctatgag
     2941 aattaatatc ataggttctg tcaaactttc tcccaactcg tcccaaaatg ttctaccttc
     3001 ttttatagta aattcattta atccatatct aagcttcctt tcttctacct cattatcatc
     3061 aagtccaatt tctggatttg tttttaagta ttttaatact tctttggtag gtttattata
     3121 atacctcata actttatccc ccttgttaat tatctataag gaattatatg cttatgatag
     3181 cttgtatatt acttttcaaa aaaaataagg tgttgattaa tcaacacctt atagtggtta
     3241 tatattatta aattttatgt tacaggcttt ataaattgtg agctatacaa atcaaaatat
     3301 ttaatcttca aaataccctt ctggatataa tggaaactta gatataagtt cttgtgcctc
     3361 tgctttacat tcatctaata cttctttatt atcaatattt tctgctacct tattcattat
     3421 ccctgctatc tgtataactt ctttttcttt cagacctctt tgagatattg atgtaagacc
     3481 tatacgaaca ccactagtta caaatggtga ttgtgggtcg tcaggtataa catttttatt
     3541 aactgttatt cctacatatt caagagcttt ttcaaattgt tttcctgtaa gattcttact
     3601 tcttaagtca accattacta tatgattatc agtacctcca cttacaatac taaaaccatg
     3661 tgaaatcagt tcagaagcta gagtctgagc atttactaca acttgctcca ttatcttttt
     3721 aaatttatct gtacttaaat ataatagtga aaaggctttt gctgcaactg tattaagatg
     3781 tatagaccct aatgttcctg gaaaaacacc tttatcgagt ttctttgcat gttctgcttt
     3841 acaaagtaca ataccacttc ttggtccaca gaaagtcttt gtcgtagatg aagaaacaaa
     3901 atctgcataa ggtacaggac taggtattac tttcgctgcg actaatcctg caacatgtgc
     3961 catatctacc ataaagtaag ctcctacttc ttttgcaacc tttgatattc tttcataatc
     4021 tattaatctt ggatatgaac ttgcccctgc aattataagt tttggtctac attcttttgc
     4081 ctttgcttca agagcatcat aatctatacg tccagtattt gggtctaccc cataaaattc
     4141 atatttataa atgtcatgta aaaaattaac tgcgctacca tgtgtcaagt gtccaccttg
     4201 gtcaagacgc atacttaaaa cagtgtcatt tggttttaat attgatgaat atacacaata
     4261 gttagctgta gaacctgaat acggttgtac gtttacatgt tctgctccaa atacttcttt
     4321 tgcacgtttt attgctaggg tttcaagttt atctgcttct tcagaacctg cttgaaaacg
     4381 agcccctgga tagccttctt ctgttttatt tgtaaataca catccactta attcaagtac
     4441 ttctgttggt gctgtacttt cagaagctat catctcaata ttgtgttctt gtctaaccaa
     4501 ctcatcagct acaatcttgt ataactctgg gtctgaaatt tttgttgttt ttaacataat
     4561 aatcctcctc ttaattaaag aatatatttt attagtttaa tcttcattga taaatttatt
     4621 ataacggtct ataaaacgta agatattcct ccaccaggtc ctaatggtat gccaataccc
     4681 atgaatagta acaatattat tatttctatt aatgtaaata ccactgagta tggaatcata
     4741 ttcgatatga cagtacctat acctatatct ttatcatatc ttctggcaaa ggccaatata
     4801 actggaaaat acggaaaaag tggacttatt ggatttgttg aagcatctcc aatacgataa
     4861 gctatttgag ttaatgctgg attaaagtct agtagcataa acatgggaac aaatataggt
     4921 gctaaaatcg cccattttgc agaagcactt cctacaaata aattgataaa tccagatagc
     4981 aatataaatc ctattatcaa tccatagcta ggtattcccg actctgctag taattttgct
     5041 cctgttattg ataaaattgt tcctaagtta ctaagattaa ataaattaat aaactgggat
     5101 gctataaatg ctaggactat atatccaccc atatcactca tactactacc catcatagat
     5161 actaaatctt tgtcactttt tatcttctta cttacttttc catacactaa tcctggagtt
     5221 aaaaatataa tagtgattat aggaactata cctttcatta aaggtgcatt actagatagt
     5281 atagaaccag tctctggatc tttcataaat gcattgtcac ctatacataa ggcaactatc
     5341 actactacta caactaacaa tgataataaa gcgtatttta tacccttatt ttcaagttca
     5401 ctcaattcct gtatatcctt atcatttgtt gaatcattat attttgtaaa acgaggagct
     5461 ataaattttt cagttacaaa tgctcctgca aaagttaaaa ctatcgctga tataaaagca
     5521 aaataaagat tcattgctgg attgccttgg taagatgggt ccatcatttg agctgctggt
     5581 attgtaaatc cagctaacaa tacatctgta gtactaagca taacatttgc tgcaaatccc
     5641 cctgcaacac ctgcaaaagc tgcaaacata cctacaagag gatgtcttcc aattcctaaa
     5701 aacacaagtg ctgcaagtgg tggtaatatt ataaatccta catctgctgc tgtatgtgac
     5761 aacatacctg caaatacgat tgctacagtt acaagttttt gtggaacttt acttataccc
     5821 tttttcatta acacttccat aaatccagat ttctcagcaa cacctgctcc aagcattgta
     5881 accagtacta atcctaaagg agcaaatgac tgaaagtttg taactatgct acctaaatat
     5941 gattgaatct gctctttctc tagtagattt actaccttta caacttcttt tgtagaagga
     6001 tgtatttctt ctactcctaa attagatata actgctgaca atattacaac aattatacat
     6061 agtaataaaa atatggttac tgggtctgga agtttatttc ctgctctctc tacaacattt
     6121 agtgccctca tcaataatcc actatctttg tttttttcct gtgaattaat tttcctcaaa
     6181 ctaatacccc ctcataaact ttatctccag aatgctctat cttaaatttt attttttaat
     6241 ttatccatat aatttataac agactgacat agtacatttg tacctaccaa tatagaattt
     6301 ttatcagctt taaatttagg attatgccac tgatatatac aatcttgttc tttgttgcct
     6361 acccctaacc agtaaaaaaa tcctggggct ttttccatga atatagaaaa atcttcccct
     6421 cctgtagaag gtattggatc aactatagca tcttctgaat acaaatcaca cacagactta
     6481 caagctattg cgtacatctc tttactattt ataacagctg gtaaatcaaa taaataatct
     6541 agctttgcag ttgcttgata tccacgagct atctcacaaa ctatattttc taaattttct
     6601 gatattttct ttcgtacttc attactaaag cttctacatg tgcctgtcat atctaccttt
     6661 tctgaaatta cattatttgc agtccctcca tttatagtac ctattgatat aacagctgat
     6721 tctaatggac ttatgtttct gcttactatg gtttgaatac ccattataat tgcacttgat
     6781 gctactatag ggtctattgt tctgtttggt atacctccgt gacctccaaa tccatttact
     6841 tctatcttaa tagtgtctac tgctgccatt aacccaccta acttaacacc tatttttcca
     6901 caaggtatat ctggatgatt atgaagtcca aatatagcat caacttttgg attttccatt
     6961 actcctcgtt ccactaacat cttcgcacct ttattgactt cttcggctgg ttgaaatata
     7021 aactttacat ttccatgaag actatctcta atatgagaca atacatatgc acatcctaca
     7081 aggcaagacg tgtgtatatc atgcccacat gcatgcatta taccatcata tctggattta
     7141 tacttaatat caacctcttc atatattggc aacgcatcaa tatctcctct tagtgctact
     7201 gtgggaccat catacttccc ttttaataat ccaacaacgc ctgtttccat tcctaaatct
     7261 aatatttcta tgtctaaact cactaataaa tcttttatag cttttgtagt cctaaattct
     7321 ttaaaactta attcggggtg catatgaata tctgtaaaaa aatctaaaat atcttcttta
     7381 ta
//
LOCUS       DACULQ010000173         7332 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.174, whole genome shotgun sequence.
ACCESSION   DACULQ010000173 DACULQ010000000
VERSION     DACULQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.174"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..288
                     /locus_tag="JA154_15505"
     CDS             <1..288
                     /locus_tag="JA154_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAT7997235.1"
                     /translation="CKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFEKLEVYFVT
                     NTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQLT"
     regulatory      381..473
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            573..1367
                     /locus_tag="JA154_15510"
     CDS             573..1367
                     /locus_tag="JA154_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAT7997236.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1391..2179
                     /locus_tag="JA154_15515"
     CDS             1391..2179
                     /locus_tag="JA154_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAT7997237.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2202..2834
                     /locus_tag="JA154_15520"
     CDS             2202..2834
                     /locus_tag="JA154_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAT7997238.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKQYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            2973..3275
                     /locus_tag="JA154_15525"
     CDS             2973..3275
                     /locus_tag="JA154_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT7997239.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3341..4096)
                     /locus_tag="JA154_15530"
     CDS             complement(3341..4096)
                     /locus_tag="JA154_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997240.1"
                     /translation="MGMLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMN
                     NININNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQL
                     LEAILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLP
                     NEIVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETI
                     LIGDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4084..5046)
                     /locus_tag="JA154_15535"
     CDS             complement(4084..5046)
                     /locus_tag="JA154_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997241.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5050..5508)
                     /locus_tag="JA154_15540"
     CDS             complement(5050..5508)
                     /locus_tag="JA154_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7997242.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5864..6220
                     /locus_tag="JA154_15545"
     CDS             5864..6220
                     /locus_tag="JA154_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7997243.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6292..7008
                     /locus_tag="JA154_15550"
     CDS             6292..7008
                     /locus_tag="JA154_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997244.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7001..>7332
                     /locus_tag="JA154_15555"
     CDS             7001..>7332
                     /locus_tag="JA154_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997245.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYVFMWKREFFSNNQSIY
                     TLMMLPQN"
BASE COUNT         2867 a          863 c         1218 g         2384 t
ORIGIN      
        1 tgtaaagatg attctctata cactggatat accagtaata tagttagaag aatgaatgaa
       61 cataaattag gaattaattc aaaatacaca agagcaaagg gatttgaaaa actagaagtt
      121 tattttgtga ccaacacaaa aagcaatgct atgaagttag aatattatat aaaaaaatta
      181 actagaaata aaaaattgtc tataataaaa aacccaagta tacttataaa tttaattgat
      241 aataaagaag attatattat tggtaaggaa atagaacaat taacataaat atttatagta
      301 acattataat gggtgcatat ggtgtaaaaa cttgacaata aatactattg taatataatg
      361 aagttataaa tatagtaaat taaaatagct aggggagcca agagctgaga ggtataccga
      421 cccttacacc tgatctggat aataccagcg taggaaagct tagtaaaaat tcaagtatgt
      481 attatttgtg atatatgcta tttctaagct atacctattg taggtatagc ttttttaatt
      541 aagttattta aattaaactt taggaggcaa caatgagtaa ttataaaata ccaacactta
      601 caatagccgg ctctgattca tctggaggag ctgggattca ggctgatttg aaaacgttta
      661 gtgcaattgg tacttatggt atgagtgtaa taacagctat aacagcacag aatactaaag
      721 gagtttttgc tgtagaggat ttaaataaga aaataataaa aaaacaaata gaggcagtct
      781 ttgaagatat acctccaaga gctgtaaaaa taggaatggt ttcaagtcct gagattatac
      841 ttgaaatagt tgagaatcta aagaaataca atccaaaata tttggttgtg gacccagtaa
      901 tgatatctaa aagtggatat tatctattaa agccagaggc aaaagaaaac ttaattaagt
      961 atttgatacc acttgcatac ataattacac caaatatacc agaggcagaa gaaataacag
     1021 gaataaaaat acataatgta gatgatatga agagagtagg agaagaaata ctacaacttg
     1081 gacctaaatt tgtacttatg aaagggggtc atttagatgg agaagcagtg gatattttag
     1141 ttggtaaaaa tatatttaaa gtttataaaa gtgaaagaat tgataaaaaa aatacacatg
     1201 gaacagggtg cacattatct tctgcaataa catcttatct agcattagga tatgaaataa
     1261 cagaagcagt aaatctaagt aaaatctata taacagaagc aataaaaaga agttttgata
     1321 taggtcatgg agtaggacca gtacatcatt tttataaatt tgagtaataa aaaaatattg
     1381 gaggtagaat atgtataatt taattaaaga tgtaaaaaaa ttaaatccat tggtaattca
     1441 ttatactaat aatgtaacta taaatgattg tgctaatgta actttagctg taggagcaag
     1501 tcctcttatg agtttttcat atgaggaagt agaagaaatg gtaagtgtag caaactctgt
     1561 agtaataaat ataggaacta tgaactcaaa tatgttggat ttattcctat tagctggaaa
     1621 agcagcaaat aagtacaata aacctgttgt tttggaccca gttggagtat ttgcaagcaa
     1681 agcgagagca gaacttacaa gtagactttt aaatgaagtc aaatttagtg ttgttaaagg
     1741 gaatgtatca gaaataaagt ttataggtgg ctttaatgta aggggtaaag gtgtagattc
     1801 atttgatgaa gaagaagatt ctactgagat aataagaaag attgctgaaa agcttgaatg
     1861 tgtagttgta gcaactggaa aaatagatat aataactaat ggtaaaggta catataaaat
     1921 aaacaatgga actgataaat taaaaggaat tacaggaact ggatgtatga ctgctagctt
     1981 gattgcaagt tttatggctg ttactgagaa tatattagaa gcagcaacaa tgggtgttct
     2041 tacaatgtct ttaagtgggg aattagctaa tttaaataat ccaccaatag gtacatttaa
     2101 agaaaatctt atgaatgcta tttatcaaat ggatatagat actttaagta aaaattctaa
     2161 tattgaattt ttaaattaat aaagagtaag ggagacttaa aatgattgat aaagaatctt
     2221 taaaaaaatg tttaaagttg tatttagtaa ctgactctga aatgcttaaa ggtagagact
     2281 tttataaatg tcttgaagat gcaatatctt ctggaataac aactgtacaa cttagagaaa
     2341 aaaatgcttc tggaagagag tttttaagaa aagctatgaa acttagagaa ataactaaac
     2401 aatatggtgt taagtttata ataaatgata gagtggatat agcacttatt tgtgatgcag
     2461 atggagtaca tgtaggacag agtgacattg atgttagaga ggttagaaag cttataggaa
     2521 acaataaaat tctaggagta tcagcaagaa ctttagaaga agctatttgt gcaaaaaatg
     2581 atggcgcaga ctatttaggt gtagggtcta tattcacaac atctactaaa ttagatgcaa
     2641 aatcagcttc gtttgaaact gttaaagaaa ttaaagaaaa agtagacatg ccatttgtac
     2701 taattggagg tataaattta gacaatatag ataaacttaa atgtttagaa agtgacgggt
     2761 atgctataat atcagctata ttaaaagctg aagatatctc taaagaagtg gaaaagtgga
     2821 cattaaaaat ataaaattgg ctctggtagt gaaaaaaatg gaaaaatttg atataatcta
     2881 agagtaaaat ttaagcaatt ataaatgata aacttttcta catatacttt ttaatcaaga
     2941 tatatgtaga tttggttaaa aaggagctat aaatgattag gatattgtta gtgtgtgttg
     3001 gaggcatgtc ttctacccta ttggtaaata aaatggagaa ggatgcaaaa aaaagaaata
     3061 tagactgtaa aatatgggca gttggagaag gagatataaa atcggagctt gataactttg
     3121 atattttact tcttggacca caacttagat ttatgcttga tgacgtaaag tctatagttg
     3181 gagatagagc tccagtatct ataatagata tggtaaacta tggcacttgt aatggtcatg
     3241 ctgtgttaaa ttcggtttta gaaatattaa aataaataat aaaagtggat tctatataat
     3301 agagtccatt tttattattt gttattaata ggcttaaaac ttagcttacc atttcttttt
     3361 ttaatctcca catagcaagt gaaaaacttg aacagcacaa tattaaaaat agtaggataa
     3421 caggcagtat atctccaata agaatagtct caccaaatat taagctacgt aaagcgttaa
     3481 taacatgggt aaatggattg agcatcactg ctacttttaa tcctccagat aaactttcta
     3541 tagggaaaag agctgaactt aaaaagaaaa taggaagtac aattgcagtc ataattgttt
     3601 cataaactat ttcatttggc aataataaac ttatagaata agctagactt gataaaaaga
     3661 aggctgtcat aaatatcagt actatcataa gtaggattcc accaatgcca gattctatcc
     3721 ttactgagaa gaatatactt actatacaca aaatagttac ttctatgaaa gaaactaaga
     3781 tagcttctaa taattgacca aggacgattg aatacctact gataggtgca attaagactc
     3841 tatagaaact tccactgttc ttcattataa agtttataat accaccacta ctacaagaag
     3901 aaaacacaac cagtacaatg atacctggta gtataaaagc agtataatta ttgatattta
     3961 tattattcat agtttggttt gcaatagaac tataaagcac aagccaaaga aaaggttgta
     4021 atatagtaac tacaaatgag attggatttt taaaacgcca cttcatacta cgccataaga
     4081 tatttaacat tcccattcct cctttaagct ttttttagaa cttgtaagtg ctaaaaatac
     4141 atcttcgaga cttggctcta caatttctat tgcatcaaat tctatatcat gttctaaaag
     4201 ccatttattt attaaagtaa aagcagttct actatcgttt acacttataa aaattgaatt
     4261 ttctcgtacg ctcataaact ttactaaacc agtactttta attgaatctt tatatttttt
     4321 tatatcttca gtattatgaa aagttatacg tagtatattt tgtctaatgt aactacgtaa
     4381 acttgatggt gttccttgtg ctaaatcctt tccattcttc ataatacaaa tattatcact
     4441 caattgttca gcttcttcaa gataatgagt agttagaaat atagttgtgc catactcatc
     4501 tctaattttt aataacatat cccacataga ttttcttgaa tcgacatcca taccaacagt
     4561 tggttcatct aaaaatagga ttttaggaga tgataccata ttcatagcta tgtccaatct
     4621 acgttttaca ccccctgaat atgatgaagt aggatatttt aagtaactag ataagtcaaa
     4681 cttatcaatt aaagagtcta ttctttgttt tgcaatctgt ggttctactt tatacatttt
     4741 actttgaaat accatatttt ccataaggga taaatgttca tcaatagaga tttgctgtgc
     4801 tacacaagca atttgagtac gtatccaaga aggattatct actaaatctt ttccaaacat
     4861 tgttacattt ccagatgtag gtttgtaaaa ggtagtcaaa atatttataa gagaagattt
     4921 accagcacca ttaggaccaa gtaaagaaaa aatttctcca gcatttacat taaagcttag
     4981 accatttaaa gctttcacac catttttata ttcttttact aaattgtcta tttcaatcgc
     5041 taacatatat tattcctcct ctttaagtgg aaatattatc tctgtaatat agttatctgg
     5101 gtttcctttt atcaacatac ctggtccttt aataaatact tctctccaag gagtttgtat
     5161 ttttatatta ttttcatgaa tatatttaaa tatcatttca tacgtttttg gcaaattact
     5221 ataagagcct gtgtgagtaa aaaataaagc ttttgtttta ggaaattctt ttgtagttat
     5281 acccatacga tttggagttt cagcagttgg aacacataat tccatttctg caacaccagt
     5341 tttttggtca acatcatagt agcaaaagaa tggtgcaccg ttagactttc cttttatagc
     5401 tttaaataca tttggaaaat actttgttgc ctcagtcata ggtccattat aacgcatggt
     5461 agccactgtg actgattcga tatttttgat ttcaattttg caattcattt gtttacctcc
     5521 taatgtgtgt tcatattctt actagatttt taagcattaa tacatataag attttgatta
     5581 gttgaaaaca tataatctta tctcgtttgc ttgttttcaa gtctaacata acataaaata
     5641 tatatcctct catttgatgc tccattttgt aaagttatta tttaagcttt actttagatt
     5701 ttttagcaaa ttaaaataaa gcaatttata aaataagtat ttacaaatta agtgtattaa
     5761 tcgtataata cacttaatag gatactagat aaagtctgaa ataaaggcgt atttttttaa
     5821 agtaagtgta ctatcgtatt aatacactag aataggagga atcatgaatt ttgaattaga
     5881 taatacaact cccatttatt tgcaaatagt aaagtatata aagagacaaa tagtaacagg
     5941 agagttgaaa ccaggagaga caataccatc aagaagagaa atggcactta atctaaaagt
     6001 aaacttaaat acagttcaaa gagcatataa ggagatgggt gatatgaata taataaatac
     6061 ttttaaaaat tatcaaagca gtgtaacagt tgatgaaaat atattaaaaa atttaaaatt
     6121 agagcttata aatgaatcac tatctgtttt tatagaagat atgaaagcaa taaatgttag
     6181 caaagaagaa gttttaaaaa taatagaaga taagtattag ttttttaaca aatagaaaac
     6241 ttagtttaga aagtttattt ataagcatat tggatgtgga ggatagtaaa tatgatagaa
     6301 ataaagaatg tcagcaaaac atataaaagg atgcaagggc ttaaaataag aaagatagaa
     6361 gctttaaaaa atgtaagttt taatatagaa aagggaaaaa taactgcatt acttggtata
     6421 aatggtgttg gtaaatcaac tatgttaaaa gcaatagcag gacttataaa cattgatagt
     6481 ggagaaattc gtatagatgg agaaaaaata aatgaaaaag tatacaataa attggctttt
     6541 gttccagatg tacaatctca ctttagcaat acaacaataa aagaaacatt tgaatttatg
     6601 gaaatttttt attcaaagtg gaataaagaa aaatcaaaag aaatgatgga catatttaaa
     6661 ttggatgaag atgaaataat tgataacttg tcaaaaggaa acattgcaag agtaaaatta
     6721 atacttggat tttgccaaga cccagagtac atacttcttg atgaaccatt tacaggaatt
     6781 gatttgttta agagggaaga gtttatagga gtaatagcgc aatatatgga ggaaaatcaa
     6841 gccattatca taactactca tgagatagta gagatagaaa gtttagttga tgaggttgta
     6901 attcttgatg aagggcagat tataacttca tttaatgcag aagaattaag agaaagagaa
     6961 ggtaaatcta tattagataa gatgagggag gtatataaga atgaataaac ttctatatct
     7021 aataaatgta gatttgagaa gaagcaataa gttttatata gcttatatat catttttttc
     7081 attaattaca cttagtttaa atttattcga aattaataaa tttaaaaatt ctaaatttat
     7141 tatgaactta gcaatagaag attttggagg tatattttat ggactaggga tacttagtaa
     7201 tcttggtttt atccaaagta tattgttctt tgggatattg ggacttttta tatactatgt
     7261 ttttatgtgg aaaagagagt tcttttcaaa taatcaaagt atttatacac ttatgatgtt
     7321 acctcaaaat aa
//
LOCUS       DACULQ010000174         7195 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.175, whole genome shotgun sequence.
ACCESSION   DACULQ010000174 DACULQ010000000
VERSION     DACULQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.175"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            101..1456
                     /locus_tag="JA154_15560"
     CDS             101..1456
                     /locus_tag="JA154_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT7997246.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(1574..2242)
                     /locus_tag="JA154_15565"
     CDS             complement(1574..2242)
                     /locus_tag="JA154_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT7997247.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(2309..2737)
                     /locus_tag="JA154_15570"
     CDS             complement(2309..2737)
                     /locus_tag="JA154_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997248.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            2889..3437
                     /locus_tag="JA154_15575"
     CDS             2889..3437
                     /locus_tag="JA154_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997249.1"
                     /translation="MYTNFNIDFNNFNKKENAIKFMLMGVLLIILGLLCLTFKTLGIK
                     LISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVLIIACILLFVFPQKIQ
                     SIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGICLILSPLFLSRFIVSI
                     MSFFIILFGLVLFFSGNTARKY"
     gene            3556..4491
                     /locus_tag="JA154_15580"
     CDS             3556..4491
                     /locus_tag="JA154_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAT7997250.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(4762..5706)
                     /locus_tag="JA154_15585"
     CDS             complement(4762..5706)
                     /locus_tag="JA154_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAT7997251.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            6024..>7195
                     /locus_tag="JA154_15590"
     CDS             6024..>7195
                     /locus_tag="JA154_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT7997252.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLP"
BASE COUNT         2602 a         1026 c          941 g         2626 t
ORIGIN      
        1 tgacattggt attataccat agtttatcat ttatattaga ataaaattat taattaatag
       61 tttcgtaatt ttataataga tttacaggga gtgataataa atggatacaa aaatatctat
      121 aaacgaaaac atgagtctag gtaaaagatt ttttaaatac ctagcaccat ctgtagttgc
      181 aatgtgggtt ttctcactat atacaatggt tgatggaata tttgtaagta aaggagttgg
      241 agaattagct ttagctgctg tcaatatatc aatgcctttt attaatttta tatttgcagt
      301 ttctctatta ttttcaactg gtgcatctac cataattgca atctatctgg ggaaaaaaga
      361 tattaaatct gctaacgaag tcttttcatt taacttagta tcaataatta tattatcaat
      421 aattatatta gctataactt tttttaattt agatagatta gcactatttt taggagctac
      481 agaatctaca attggtatgg ttaaagatta tttaggaata ataatatttt ttaacggatt
      541 ctttatagtg tcttattctt tagaagttat aataaaaact gatggctttc caatattagc
      601 tactgttggt gtaataatat ctgctcttac aaacatcata cttgattact tgtttgtaat
      661 tgaatttgga tggggtgtaa aaggtgctgg tattgcaact ggtttgtctc aagtcttttc
      721 tactattttc ttcttaatac attttttaag aaaaaactct actttgaatt tttcaaaatt
      781 tagaattgat tttaagacac ttagaaaaat tgtatttata ggttttcctg attcaactac
      841 tgaattaagt tgtggtatag ttgttttact ttttaactta agtcttacta aatatattgg
      901 agaaaatgct cttatttatt atagtgtcat aaattatata aatacattag tacttatgac
      961 tatgatgggt ataactcaag gtatgcaacc acttactagc ttttattatg gtgcaggaaa
     1021 tatagataat gtaaaaaaat tattaaaaat ggggataaaa gccacaataa tagcatcagt
     1081 tgctgtattt gcaatttgta tggctttttc aggaccaata gtctctttat ttatacatcc
     1141 agaagaaaca atgctattca atgaaggagt tcgtgtattt aaaatattct ctatatcttt
     1201 cttattggta ggaattaatg taataatttc tggattcttt gtatcagtag aaaaaccatc
     1261 aatttctact gttatatctc ttggtagagg gcttgtaata gttgttttaa gtctaatttc
     1321 aatgatacta atatttggtg gacaaggaat atggatgact actattgttt ctgagtttat
     1381 atgtcttatt ttatcattag tttttctaaa aaagaatttt tctacattag actctaatct
     1441 aaataaggtt gcttagatga taagagtgac aatatatgaa aatatctaaa taaaaaatac
     1501 ctattataca atcttgcaaa tattaaacct ttgcaaaaat taggattata taataggtat
     1561 tttatttatg taattatttt atttctaaat atccatagcc atcttttatt aaagttcctt
     1621 ttacttcata ttctacaggt tttttaaata ttggaccatc tacgacaaat gtatgatact
     1681 ctccatgttc tccacatggg tctgcaccag cattttttat cttctccaca agttctctag
     1741 ttaatttttt tcctaaaaat tcctctccta agtgctttaa gtttactttt gttacaacac
     1801 tacaaaatcc actgtctata aattcatata ctaaatcctc tctactctct tgccataatg
     1861 gtaaatctac tttcatacca actgcttcac atctatcagt atcccatttt ctatgacttt
     1921 ctatatctat atctccatat gcacatatcg ttgctcccat gtttttagct tttattaaag
     1981 ccctctcaaa gctctcttca tatccttcta cgccacactc agcaactaat aaaggtaatc
     2041 ctatgttgct acttacttgc ttaagcatat tatattctaa attatgtgtc catgactttc
     2101 catgttcttc acttatagtt gtcaagagtg ccacaggctt atatcctttt ttcaacattc
     2161 gatttaatgc taatatacta tccttaccac cactaaatga cattacaaat ttttcatcag
     2221 ccaattttaa cacctctttc atctaacttt gttttattaa cataattata ccaaattttt
     2281 gagttgcata gcaaattata ttaaattgtt aattcactat aaagaatgtc cctaatatta
     2341 aaagtataac ccctatgtat ttgtataaat gcattgtttc ttgtcccaaa attacgctat
     2401 caataaaaaa tccaactaaa acttgggata caagagttaa tataagtgta tttgatactc
     2461 ctaaatttgg aattgccttt actgtaaaat atattattag acctccaaat actcctccta
     2521 ataacaactt ataattaaaa ccatttatag atactagaca ctttaagtca cctgtaacta
     2581 ttatacttgc caaaaaaaat attgctccta cagttaagct tataaaggtg gccactaatg
     2641 ctgttgtact ttttcctagc tctccattta taaaagcttc aagagctgta gaaccacctg
     2701 ctaataatgc aaatattatt gcccataatt ttgtcatgga caaacctcct tctatataat
     2761 tctattaata gaagtttatg atacaaccta ttaattaatt actaagtata gtttaatttt
     2821 tatacactag gctttatttg gatatgaatt tctgttataa ttgttctatc gattttggag
     2881 gtgattttat gtacacaaat tttaatatcg attttaataa ctttaataaa aaagaaaatg
     2941 ctattaaatt tatgttaatg ggagtattac tcattatatt aggattatta tgccttacat
     3001 ttaagacatt aggtataaaa ttaatttctt ggacttttgg tattgcactt ttattctttg
     3061 catatttaaa tttaaaaaat attaatgagt taaaaagata tgctactaaa gaagaaatta
     3121 aaccctctat taatatccaa tgggttctaa taattgcatg tatattactt tttgtatttc
     3181 ctcaaaaaat acaaagcata ttttcattat ttttaggatt ttatttaatc tttaatcaac
     3241 tagtagcact tgtaaatagt aagaataatc cttattctaa atttacaact tggaatatag
     3301 ttaagattct atttggtata tgccttatat tatctccact ttttttatct agatttatag
     3361 tatcgataat gtcttttttc ataatattat ttggactagt tctatttttc tctggaaata
     3421 cagcgagaaa atattaatta ttttctcgct gtatttttat attacttaaa atagaatttg
     3481 tttaagttat ctatttgtgg taatatgttt tttgtacaag ctcagcgtac aattttatta
     3541 gaaataggtg attttttgga taaatttaaa tatgcttttg ataataaaag atatcatact
     3601 tggaattact atcttagaaa tacttttgga gaaaaagttt ttaaggtatc tataaatgca
     3661 ggatttactt gcccaaacat tgatggaagt cttggttatg gcggatgtac ctattgtagc
     3721 aaagaaggct caggtgattt tgctggaaac cctaaagata atcttatcag tcaattttat
     3781 aatataaaag aaatgatgtt aaaaaaatgg cctcatgcaa agtatattgg ctactttcaa
     3841 gcctatacaa atacgtatgc tcctcttgaa gttttaaaag aaaaatatga gactatatta
     3901 gaacttgaag atgtcatagg tctttcaata tccacaagac ctgactgttt gccagatgat
     3961 gttgtagaat acttatctga actaaataaa agaaccaatt tatgggttga acttggactt
     4021 caaacaattc atgattctac atctaaaatt ataaatagag gtcatgatta taaaacattt
     4081 gttgaaggtg ttgaaaaact aaagtctaaa aatataaagg ttgtcgtaca cattataaat
     4141 ggtcttcctg gtgaagatta taatatgatg atggaaacag caaaggcagt tggtaaaatg
     4201 ggtgtagacg gtataaaaat ccatctattg catgttataa aagatactcc tatggaaaag
     4261 atgttacaaa atggtatgtt aactcttatg gaacaagatg aatatatcaa tcttgtctgt
     4321 gaccaattag aaatacttcc tgaaactatg ataatccata gactcactgg tgatggtaag
     4381 agggatgaac tagtaggtcc tatatggagc ctaaaaaaat gggaaatttt aaatcaaatt
     4441 gatgatactt taaaggctag aaattcttat cagggctgta agtttgttta atacaaaata
     4501 ataactatag aaatggttat ataaatatag tcctttctat agttatttag tatttacaaa
     4561 acaaaatatt aatttgtaca tttatacaat atataataaa aaatagatag tctaaactgt
     4621 atctacttat attttatttg tactgtccac tttattctat aaaatttata aatagttatt
     4681 ttttaatacc aaatataatt tggtataatt tttgttggac tctaaaatgt ataccaataa
     4741 atccttttta agtatataat tctaaatact ttcagttttt tcaatctgct ttaacttgtt
     4801 ggacttataa aatattctta tttcatcata agaaagatta agtgctacta ctgcagatat
     4861 taacatcaac atcactccaa ataacttccc taaggaaaaa ctttctccta atatagtgca
     4921 accaattatt actgaaccaa ctaattctac tgatgccaca acacctgcaa ctgacaactc
     4981 tacaccttta gatatacctg ctgcataaaa aatatatgca agtgcagctg gaacaatgcc
     5041 taacattagc atacaagata aaacatctaa atccttagca taatttaaga tttcccaagg
     5101 tcttgaagaa ggaatcataa atatagctcc aaataagaaa ctgtatacta atattgttga
     5161 acttgaaaat tcctttaaag catttttact aatagttggc ataagtgcat atgctattgc
     5221 tgataatacc ccaagtaaaa gtccaaaagc gtttaaacct tgaaaatcca aacgtccacc
     5281 agtcactgcc ataattgcac caataaaaca aagaataagt gaaaataatt tgcttctagt
     5341 aatattttct ttataacata tttttgaaaa tattgctaaa aacagtggtg atgtatatag
     5401 taaaactgct gcaattgata cacccgcaat gtcaattgct ttaaaataac ataaattaaa
     5461 cattgcctga catataatac ctattattac tgaatatata attccttttt tgcttatctt
     5521 taagatttgt ggtgtcctta tagatgaata tacgcctaaa actataaatc caaaaaacaa
     5581 tcttgtaaat gctacttgtt ctgatgtcag tccatttccc attaaaaact ttccaaataa
     5641 tccaagtgta gcccataata accctgctat tgctataaat acataccctt tacttttttc
     5701 tcccatttta aattcccttc tatttttatt tagtcaattt acactcttct ttttaagcaa
     5761 aaactatgcc atcttaaatc aatatatatc gactctaaaa ccctagtctt tccaataata
     5821 ctaataaatt ttcaattttt ttatatttat ttttcactta ctttaagttg atttagactt
     5881 attttaattg taaaatgagt tatatataac ttatttaaag ttaaaataga ctgaaactta
     5941 tatataaata tcaaatttta agtatattaa tcaattaaac ctattttaat atatcattaa
     6001 aaatttagga ggaaaatata aaaatgaatt tagatttgga attttatcaa aaaatattag
     6061 aagcttccca tgatgaaata tgtgtttctg atgataaagg aataataata tattgtaata
     6121 aagcatttga agaaaattat ggtctaaaaa aagaagatat tttaggaaaa aatgtttctt
     6181 ttttagaaga ctctggatat tcaacaaaaa gccccatacc aattgtatta aaaacaaaga
     6241 ctaaattttc actggaacaa gatactcaaa cagggaaaaa gcttatcata actgctaccc
     6301 ctatatttga tgaaaatgga aacttagaat ttactgttga aaattgtaga gatataactg
     6361 aacttaataa tataaaaaat aaacttgaag acacaaaaaa gcaagtaaaa aaatataaat
     6421 ctgaagtaga aacactatac agaactgcac ttagaattga ggatactgta attatggacg
     6481 gaatagttat gagaccaatc ataaacacag taaatcatgt ttctaaaacg gatgtaagtg
     6541 tacttttatt gggtgaatct ggaacaggaa aaagctcact tgctagatat atacaccaca
     6601 acagcaatag atctaatggc ccatttataa caataaattg tgcaactata tctccacaac
     6661 ttcttgaatc tgaacttttt ggatatactt caggtgcttt tacaggtgca agtactaaag
     6721 gtaaagttgg gctcgtagaa ttagctaatg gaggaactct ttttttagat gaaataggag
     6781 atataccaca aaatctacag gcaaaatttt tgcaattaat acaggataga acttttactc
     6841 cagttgggag cttaaaaaat aaaaaggtcg atataagaat tatatctgct acaaatgttg
     6901 atttagtttc aaaagtaaaa gaaaaaaagt ttagagaaga tttatactat agattaaatg
     6961 ttatagaaat aaaattgcct cctcttagag aaagaaggga taatctagtg gaaattataa
     7021 aatattattt taataggtac tcaagtgact tcaatctaaa taaaactatt tctaaagaag
     7081 ctatggatgc tattgcaaat tatagattcc caggcaatat aagagaatta caaaatataa
     7141 ttcaaaaaat cttgcttact tgtacagata atcatataac aatagatgac ttacc
//
LOCUS       DACULQ010000175         7102 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.177, whole genome shotgun sequence.
ACCESSION   DACULQ010000175 DACULQ010000000
VERSION     DACULQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.177"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            76..3264
                     /locus_tag="JA154_15595"
     CDS             76..3264
                     /locus_tag="JA154_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAT7997253.1"
                     /translation="MNNMKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDL
                     QNNLLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVA
                     IVIKSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVN
                     LDVLINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFA
                     DEDEKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEY
                     HPKILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDY
                     SKIYQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYF
                     DMIDGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDL
                     FTFLDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPR
                     EYKDILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKAL
                     YINDVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKV
                     LDYYKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIK
                     VLLIHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPV
                     ALVTESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDN
                     VKNLKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDK
                     NLKKDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFS
                     QFTSVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTG
                     LNLTSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQES
                     KKELINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3549..4313)
                     /locus_tag="JA154_15600"
     CDS             complement(3549..4313)
                     /locus_tag="JA154_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7997254.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4469..4702)
                     /locus_tag="JA154_15605"
     CDS             complement(4469..4702)
                     /locus_tag="JA154_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997255.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4776..6269)
                     /locus_tag="JA154_15610"
     CDS             complement(4776..6269)
                     /locus_tag="JA154_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAT7997256.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYNSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            complement(6597..>7102)
                     /locus_tag="JA154_15615"
     CDS             complement(6597..>7102)
                     /locus_tag="JA154_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT7997257.1"
                     /translation="GGKSTGVNGIVDEYNYNKKLAPMLAEMLISQGNTVDVIICPERH
                     FMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGTEGLEYAKRVSNRL
                     GELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANESGYDYIARLITEGI
                     LNKDINM"
BASE COUNT         2608 a         1033 c          796 g         2665 t
ORIGIN      
        1 tctgccttaa ttttagtctt aaatttagag tgtaaattga atgcatttag attataatat
       61 atacagtgag gtaaggtgaa taatatgaaa atagatatga tatataaact aataagaaga
      121 aatacagaac catcaagatg gtcaagaggt gtaaaatatt atactaatca attagttgat
      181 tatatagaca tagatattga tgacctacaa aataacctgt tgacatttcg tggtggagtc
      241 aattctgaag ataattatag cttttatttt aattcaataa ctatagatat aaagcacttt
      301 aagataattg aaaatgattg tagttgcgct gactgtcaat caaagggcaa aagtattttt
      361 atatgtaagc atatagttgc tattgtgata aaaagcttaa aacaaataaa aaataaaaaa
      421 gatgttcctc ctattctaat agatatatta ggtgcagata aggataagct ttctaataca
      481 aattctaaaa ccagcttaga tttaagcaag caattacttt ctttgttaga caatactaaa
      541 agagaaaggg ttaatttaga tgtattgata aatgtggatt caaataaagc aattgctgaa
      601 tttaaaatag gaattgataa aatgtacatt ttaaaaaaca ttactgaatt tgcatattca
      661 aaacttaatg gatatgaatt agattttggg aagaaattta cttacaaccc caacactcat
      721 tattttgctg atgaagatga gaagttagta gatattatag aagaatatgg gacaaatctt
      781 tcatacaact tttcttcatc acattcaaaa aaatttatgg agataaatgc aagctcttta
      841 aaacaatttt taaatgctat taagtacaag gattttaccc ttattttttc taaagaggaa
      901 tatcatccaa agatattaaa tgatactcta cctgtaaggt ttgattttaa aaatatagaa
      961 gataaaatag tcttatattc taaagattca ctacctgttc cacttactaa aaaaaatgat
     1021 gtacttttat atgatggcaa tatctattta ttatcacata aaaaagctca tgattattca
     1081 aaaatatatc aaatattatc aaagactaaa gagttaaaat ttgataaaga ggattctaag
     1141 gatgttttag gattgctagt tcctagatta aaatctatat ctcaagaagt tcatttagat
     1201 gataacataa aaaataatat aacaaaagat tttaaatctg agttctactt tgatatgatt
     1261 gatggcaaca tatgttgtga tgtaaagtat atatatgatg atgaagataa aaaatttgtg
     1321 ctccccaaca tacaaaagga agctactatt gaaaataaac ttacttcttc acaatttaca
     1381 aaggaaaata atcattatgt gtttaaagga actgaccatg acctatttac atttttagat
     1441 ttacaattag aatctctaaa ggaatttgga gatatatatt attctgagaa gtttaaattg
     1501 aaaaaaatat ataacgcttc atcaattcaa gcttcaatca atcagactaa ccagaattac
     1561 ctagaattta attttaatat atctgatata aatccaagag agtacaagga tattttgaaa
     1621 gcttttcaag aaaaacgtac tttttataaa cttaaagatg gaaactttat agatttatca
     1681 gaaagagaaa caaaagattt ttttgaatta gttgaaaatc tagatataat ggataaatca
     1741 aaaaataata aaattcataa aaataaagct ctatatataa atgatgtaat acgtagtaaa
     1801 aatttaaagt ttatagatgg taaaaaagaa ctagacagta tatgtgaaaa atttagaaat
     1861 tttgatagca taaatttaga aattcccgca aatttaaatg caaaccttcg tgactatcaa
     1921 ataaatggcc tcaattggtt taaagtttta gattattata aatttggtgg tatattagct
     1981 gatgaaatgg gtcttggtaa aacaatacag actattgcat ttttattatc attatctaat
     2041 aaaaaaagtc ttattgttac accgacatca ctcatttata attggaaaaa tgaatttgaa
     2101 aagtttgctc cagatataaa agttttattg atacatggaa ataaaagaga tagagaaaaa
     2161 tgttttatgg aattagaaaa ttttgatgta atacttacaa catatggtac tcttagaaat
     2221 gatttagaaa aatatagtga aataaagttt gattattgta tattagatga agctcaaaat
     2281 ataaaaaatc cagtagcttt agtaactgag tctgtaaaaa gtataaatgc agaaaataaa
     2341 tttgcactca ctggtacacc tatggaaaat aatctactag aattatggtc tatatttgat
     2401 tttataatgc ctggctatct atatagtaaa gcaaagtttc aggaattgtt tataaataaa
     2461 gaagataatg ttaaaaacct gaaaaaattg ataaaacctt ttattcttag aagaagtaaa
     2521 aaacaagtta tgaaagaact tccagataag attgaaaaaa acttttttgt tgaattgaat
     2581 aaagagcaga aaaagatata tagtgtatat tcaaaagata tacaagataa aatgaaagat
     2641 aaaaatctaa agaaagataa aatagttata ttttcttatt taactaaact aagacaactt
     2701 tgtttagacc caagtatagt agtaaaagat tacaataaaa aaagttcaaa aattgaaaca
     2761 tgtttagaaa tacttaggga tagtataaac gaaaaccaca aaatattatt gttttcacaa
     2821 tttacttctg tgcttaagaa cataagtaag gaacttgata agtataaaat taaataccat
     2881 tatatagatg gtaaaactaa tgccaaagaa agacttgaac ttgtagatga gttcaataat
     2941 agtatggata aaaaagtttt tttaatatct ttaaaagcag gcggaactgg tttaaatctt
     3001 acatctgctg acatggtaat tcactttgac ccatggtgga atccatcagt agaaaatcaa
     3061 gctagtgata gagctcatag atttggtcaa aaaaattcag tacaagttat aaaattgatt
     3121 gctaaaggga ctatagaaga aaaaataata aaattacaag aaagtaaaaa agagcttata
     3181 aatcaattta taaatggaga actttctaat gaaggtgtat taaaaagtct atctgatgag
     3241 gagataataa acttatttaa ctaaattatt taatcctaca gcttaactat ataaatttgt
     3301 tctaacattt atatagttaa gctaaaatat ctgaataaat aaaatttaat attgttattt
     3361 aatcccaact ttacaatcta atgatatctc tttctataca tattgcatat acaaatgtaa
     3421 taacttgact attagaatat tttgttttaa caatactatt tcatcataat aatgaagtat
     3481 atgtacactc acttttgtaa gttttgcaat ccattttaca gttttcatta ttataaattt
     3541 tggttaaatt attcctctaa aaatttcttt gctacatttg aaaaatctaa tccattcgat
     3601 aaaattgcag gatgaccacc tttatcaaac aaatacattt taccattttt aatcttttca
     3661 agaagtaatg aaaaagtatt ttcaaaaaaa tcactagata ttagagatac aaactcatct
     3721 tctctacttc ctgttaaaag cacctctggt tgcatagttt ctagaggctt atgaaaaaat
     3781 tttcctatag ttttataatg ttcaaaaata gcttctgtat cattatctac aactttttcc
     3841 cagttttctc cttgattata aatataaaaa gcttttgcac catcatcttg cttagatgct
     3901 tcacgctcta ttttcacatt ttgtacaaat gactctagag gaacttctcc ttcaaaacta
     3961 tctgcaatta ccttattcac taaatcaggt ctctcaagtg caacatttaa tgcaacaaga
     4021 gctcctccac tactaccaat tatgtttact tttttatagt tcattgcttc taaaaaacat
     4081 atcacttgca tagcctcatc aaaccataag tcagcagaaa atttatcgac catttgagat
     4141 tttccatgac ctacaaaatc aattaatatt actttatatt catctttata aaaatcgatt
     4201 atatcattaa acatcttgga agaggctgta tttccatgta aaaaaagtaa tggtgtacca
     4261 tccccaattt cattatagta gcactgtttg tcgttatagt taaaatatgc catatatttc
     4321 ccccctttaa tatccctata ggtatatatt tattacttat atttaaaatg actattccat
     4381 tagccactta aaaatagtat aacttagaat gtagataaaa tcaacattcc ttgctaattt
     4441 atctattgtt tgtctaattt attataattt aacttctgta aaatttcttt tttgtacgaa
     4501 aatattcctt ccatttacaa caatcattca aatgaagtat aacatgacga gtaggaggca
     4561 taagcaaaat tccagcaata tctttattcc cccaaaagtc caacaaccaa atagaatctt
     4621 catgctttat tactcctcca gtcaccaaaa tcctttctaa gtcaattgga gagacctttc
     4681 gtttcatatc ttcagctttc aaatccttaa caacttctat tgtgtttttt gcaacttcat
     4741 ttcgataaca aagcaattca tcaatattga ctaaattatc gtatatctct tttcacttta
     4801 accatttttt tctttatagg tatctcctta tattttttta atatcaaatc tcccttataa
     4861 tttaatatat ctacatatga acataaatct tgaacttcaa ttacacctat atcatcatta
     4921 gttatacctt ttatattact aaatgcacca actatatcta aaactcttat tttcttcttc
     4981 ttaccagcat ttaaatataa tttaacaact tcactttcta tatttagttt tatatcttta
     5041 ctattgtaag aatttttatc aacttttttt ctctttgata ctttttttag gattcctttt
     5101 gaatattctt caaacaaaat tttgccatgt gtaattctac ttttttctat atcttttaat
     5161 tcatttattt tatatcctat atacgtttct atattctcta tatatttttc atccttttca
     5221 gttactatag taattgcctt tccactattt ccttttctcc cagttctacc tattctatgt
     5281 atatagtttt ctttatcttg tggcacatca taatttatta ctaaagaaat atcatcaata
     5341 tgtattcctc tggacgctac atcactacta actaatacat taaatttttg gtttttaaaa
     5401 tccttaatta caaaaattct tctttcttgt gataaatctg catgtaactc tcttattaaa
     5461 aaaccttctt tactcatctt gttatataat ttagatactt tctctttggt attacaaaat
     5521 attatcacac tctttggatt ttcgctgtat attaattctt ttaaaaggat gtacttctct
     5581 cttccatttg ccttgattat tttgtcatct atttgtcttg ttttttgatt agtatcgctc
     5641 tcattttctt ctatatttat aacactgtaa tctagcatat atttttcaca tatatatttt
     5701 atctcttcat ctattgtagc tgaaaaaaga cttactattt tctccttagg taaatttaat
     5761 aatatttttt ccatttgttc aacaaaacct ttattaaaca ttttatctgc ctcatctatt
     5821 actaaatact ttacattttc caacttaata gaacctctat ttatatggtc aagtattctg
     5881 cctggagtag ctacaacaat atgaaccctt tgtttcaatt cagctatttg gtcttttata
     5941 gattgtttac caaatatagc actacatctc accttcttta gtctccctat atctgatatc
     6001 tcatctttta cttgcaaggc taattctctt gttggaacca ctataagagc ttgaatatta
     6061 ttgtaatcta catttatatt ctcgcataaa ggtattgcaa aacttgctgt ctttccactt
     6121 cctgtttttg acctaactac taagttttga ccttttaata attttggaat aacttctctt
     6181 tgtactctag aaggtatatt atatcctaaa ctcttcaatg acttaagtat cttttcattt
     6241 aacttaaatt tttcaaatgt atatttcata tattaccacc ttattatatt tatcttatct
     6301 atatattttt ctacattata ttattataat acaaggttct actattttta tattatttat
     6361 tagactgaag ataaaaatat atactctata aacctatagt ttagtagcaa attaattaaa
     6421 tttttatatt taaatacctc cttaaatata ctgataaatc aatattttca aggaggtatc
     6481 tcttgtcaat aatcaaacta atctatcttt tgaaagttta aattatacta atacattgta
     6541 tttttattta tatctaatat taaaaggtat taatcaatac ctaaatcacc ataatctcac
     6601 atatttatat ctttattcaa tataccttct gtaatcaatc gtgctatata atcataacct
     6661 gattcatttg ctttgttaca atcattcaca ttatcacaga aaaaactttc aatctgtatt
     6721 gcaactggat ttgtatttct caatatatat aaattttctc tttttttagc tcctctattt
     6781 tcaaataatt ctccaagtct attagatact cttttagcat attctaatcc ttcagtgcca
     6841 tagtaaagca cttctgtacc acattgtgtt ccatcagctt tatttaaatg tagctctact
     6901 aataaatcgt atttaccact gtttactttt ggaactctat agaaaaattc ttctctttct
     6961 gacataaagt gcctttctgg acaaattatt acatctactg tattaccttg acttataagc
     7021 atctcagcta acataggagc taatttttta ttataattgt attcatcaac tataccattt
     7081 acacctgtac ttttaccacc tt
//
LOCUS       DACULQ010000176         7050 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.178, whole genome shotgun sequence.
ACCESSION   DACULQ010000176 DACULQ010000000
VERSION     DACULQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.178"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1950)
                     /locus_tag="JA154_15620"
     CDS             complement(<1..1950)
                     /locus_tag="JA154_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAT7997258.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDD"
     gene            complement(1963..3627)
                     /locus_tag="JA154_15625"
     CDS             complement(1963..3627)
                     /locus_tag="JA154_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAT7997259.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(3925..4422)
                     /locus_tag="JA154_15630"
     CDS             complement(3925..4422)
                     /locus_tag="JA154_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAT7997260.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            complement(4451..5830)
                     /locus_tag="JA154_15635"
     CDS             complement(4451..5830)
                     /locus_tag="JA154_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HAT7997261.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(6135..6281)
                     /locus_tag="JA154_15640"
     CDS             complement(6135..6281)
                     /locus_tag="JA154_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HAT7997262.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(6311..6889)
                     /locus_tag="JA154_15645"
     CDS             complement(6311..6889)
                     /locus_tag="JA154_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator"
                     /protein_id="HAT7997263.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
BASE COUNT         2313 a         1278 c          656 g         2803 t
ORIGIN      
        1 atcatcttca ataatattgt aatttatgtt aaaatccaaa tctataagtg atatatctga
       61 cattgctcta taaaatccat ttatagaatt agatattatt agtgtatctt tttcaaaata
      121 atcaaatata tttatatctg tatttccatc aaataattca aaatcagaca cagatatatt
      181 atacaggctt tgagtatctt ttggtattat ttttgacatt aatttcaatg aaagtttgtc
      241 attacttgca cttttaggtc gagccaatcg tatatcttta agtaaaaatt gtactttcct
      301 atttcccata tagttatttt catctaattg gaatagtaca tctaccttgt ctcctacttt
      361 atacattgat tttaaatgac tcatattaaa tcctatacat tcatagcttt tctctttttc
      421 tatgcttaat ttcagatgtt gttgattctt acctataact ttcaaatctt ttaaaatata
      481 atttctaact ataaatctag gattaggatt atttaatcca aagggttcta acttatgtaa
      541 ttcttctaca agatttaaat ttattacatt ttcttgtaat tcaaactcaa catttacatt
      601 ctctatcata tcatcttcag ttaaattata atctgcaaat ttatttattt catttgctaa
      661 aatttctaca ttgtctgaat ccaaggataa ccctgctgct tgttcatgac caccaaactt
      721 agtcatcaag tctttgcact ttatgagagc ttcaaatata ttaaatcctt ttattgacct
      781 cgctgaacca gtagcctccc cattttctat acctagaaga attgttggtt tgtagtattt
      841 ttcagttaac tttgatgcta caattcctat tattccatgt tgccaaccct ctttagctaa
      901 aaccaacacc ttgtcatcat tgtatctgct attagacttt agcatttctt cagcttcatg
      961 atacatctta gcttctatca tctgtctttc attatttttt tcttccaata tactagctat
     1021 ttctactgcc tcttcttgtg attgagtagt aaatagctct actcctaagt aagaatatcc
     1081 caatcgacct gaagcattta tcctaggacc tattgcaaaa cctatatgag aagaacctat
     1141 cttatcactt tctacaccac aaacttttat aagctccctt aatccaatat ttttgccttc
     1201 tttcattgat tttaaaccat tttttacaat tattctattt tcatctataa gtggaacaat
     1261 atcacaaata gttgctagtg ttactatttc caaataatta tacatactag ttttaaattc
     1321 ttcttttgga gtcaaggctt gaatcatttt aaatgctaca ccacatccac atagtgaatc
     1381 aaatggataa ttacaatctt cttgttttgg atttattact gcaaaggctg atggaatttc
     1441 actttgacat tcatgatggt ctgtaattat aacatctatg cccaaatcat tcgctgtatt
     1501 gacctcagac actgatgtaa ttccacagtc aacacttata atcaagtcac atcccctaga
     1561 gcttataagc ttaatagcat cttcatttat accataccct tcttctaatc tattaggaat
     1621 atagtaatca acattgtaac ctatactctt aaaatataag cacaagatag atgtagatga
     1681 tacaccatca acatcataat ctccgtaaat ccaaattttt tcgttcttct caactgctaa
     1741 ctttattcta tcaacagctt tttgcatatc tttcattaaa aaagggtctc ttaaataatc
     1801 taaagatgga ttcataaata tttctgaatc tttttcagta catatccctc tattttttaa
     1861 aatttggcag atttccgggg aaatattaag ttttttgcta aattcacttt cctctatctt
     1921 acctttatac ttcaatatcc attttttcaa atttaattcc ccctaatctt ttttgtattt
     1981 tgacctaata cttcttataa ctaagcaaag gcctattata aaggatataa agaaacccgc
     2041 tattgcagta aattttatcc atatattatt ttctatattt ggagatgcaa ttataagaga
     2101 agaacctact ataatagatg ccaacactaa actcaaagat atttgagatg ttagctctat
     2161 tatacaattt tctaacctcg tcattttaac atcttcaatt tgcattttta tattatttct
     2221 ttctatattt tttaaaattg cttttaattg ctttggaata gtttttatgt ccagtaatat
     2281 ttcttctaca ttttgtcttg aacttaaaac aacattttta gggttaaatt tactcttata
     2341 atgatgtttc ataaactctt ttcccataga accaaaagaa aaatctgtgt ttaaagttct
     2401 acttgttcct tctaatgtta taactgtctt tgcaagagtt acaaattgtg caggcatgac
     2461 tattttatac tgtctaaaaa acctaaatag ttcatttaat atatctgtta catttatttt
     2521 ttctatagaa atatcatagt aataatgtat tagataaagt aaatcctgtc taagtccctt
     2581 aatatcagcc tctccattta atgcatccat ttctatcaat agatatatta tcttgtcaac
     2641 attcttttct acacctgcta atgcaatttc atttaattgg tttaaagttt tattatcaat
     2701 aattccaatc attccaaaat ctatatatgc tatcttattt ttagcaacta cgaatatatt
     2761 acctggatgt gggtctgcat gaaaaaatcc atgagataat acctgtgtaa aaaatgacct
     2821 aactcctatt tcaacaatag tttttgtgtt ataacctagt ctccttattt tttctacatc
     2881 acttaacttt gtaccattaa ctttctccat tactaaaatt ttcttagtat tatattctga
     2941 atatacttta ggtatatata cttcatcact atttttaaat atcctagaga atttagttgc
     3001 atttatagct tcaaatgtat aatcaagttc cctcattaat tgagtttgaa attcttcaat
     3061 catctgtact aaatctaaat taaaatcctt ttttaaatct tttaaagtat ttgcaatagt
     3121 tctaagaatc tctaaatcag attttattat tttctctata tttggtcttt gaatttttac
     3181 gattacttct tctcccgttt taagtacacc ttcataaact tgaccaattg aagctgctgc
     3241 tattggttct tctttaaatt ctaagaatat ttcttctata cttaaaccca cttcttcttt
     3301 aaatatatca atagctatat ttgaatcaaa tttctctaca tcatctctga gttttgatat
     3361 ttcatcaatt atatcctggt ctaacaagtc ttttcttgta ctcaatatct gtcctatttt
     3421 tatatatgta ggtcctagtt cttctaaaac tcttttcatt ctctcaccag ttgtcatatt
     3481 ttttatttct tctggtgggt caaatagtgg tattttatag gcaattcctt ctatatttag
     3541 tctttctaca ataaaactaa aaccatactt tattaaaaca tatccaattt ctctatatcg
     3601 ctttaaattt ctataactta ttctcaaatc ttttcactcc taagataggt atacccaaaa
     3661 acttacacct taatttatcg tatatgacat tatttcacca attgatattc taatatccta
     3721 tatcctcttt aatatttaat tttatcacat atactaccaa ttttattaaa attatttaac
     3781 tcagttaatt tttatttttg acattttata tctggtttaa tcaaatactt tttgaaaact
     3841 aaaatatcaa caaaatctaa taaaataaat tagtggttgc ctcaaaacag atttaaatat
     3901 gttttgaaac aaccactctt taaactaata tttttgattt tgtgctgcct ctaaatacca
     3961 ttcataagat tcatcaatat attttttatc ttcttctgta agctctctaa caaccttacc
     4021 aggagaaccc attatcaaaa cacctggtgg aaacttctta ttagatgtaa ttagtgtacc
     4081 tgctcctatc agagtatatt ctcctatttc agcattatcc aatattattg aacccatacc
     4141 aattaacaca ttatcactaa ttttacaacc atgaactata gacctatgcc caactgttac
     4201 attgttacca attatagttt ctgtatctcc atgcactata gaacaatctt gtatattagt
     4261 gttttctcca atagttattg gtccttcatc acccctaact actgcattgt accatatact
     4321 tgaatctttg cctattttaa catttccaat tacatctgct gattttgcca caaatacact
     4381 ttcgtcaatt aaaggtttat cttctaaata atctctaatc atatataaac aactccttta
     4441 aaatataaat ttagttggtt gctccttcta acattaactt agcttgtatc attatagagc
     4501 actctgtacg ttttctttgc atttcacatc ctaattcata tggttttgat atgtcattat
     4561 taaagtttat cgcatttgca tgacatccac ctgaacagta aaacttattc caacattgtt
     4621 tacactcttc tttagtatat acatgagctt ctctaaacat attttttaaa ttttctggaa
     4681 gtactatttc ttcatcaaat atatttgcca ttttaaattc ttcatttcca acaaattgat
     4741 gacatggata tatatctcca tttggagtta cagaaagata ctcatttcca gctccacatc
     4801 cagttattct ctttattaca catggacctt gatttaggtc tatcataaaa tggaaaaatt
     4861 taaatccatc tccttgtaat tgtctatctg catactctac agcaaatttt tcgtactctt
     4921 caaatatctt aggtaaatct tcttctctaa gtgcatatgg attactctca tctccaacaa
     4981 ctggctctac agaggttaat ttaaatccta aatctgcaaa atgcataaca tcttttgaaa
     5041 aatctaaatt atctcttgta aaagtacctc ttatataata atacttatct tttgctcttt
     5101 tttccacaag tttcttaaat cgtggtaatg taatatcata acttccttta tcatttaaag
     5161 ttggtctcat attatcatta acttcttttc ttccatctaa acttaaaact acattatgca
     5221 tattttcatt tatataatct ataatctcat catttagcag tacaccattt gtagttattg
     5281 taaatcttat atttttatta taatcttttt ctacacttct tccatagtca accagttgtt
     5341 ttacaacctc aaagttcatc aaaggctctc caccaaagaa atctatctct aggttctttc
     5401 tacttcctga attagcaatc aaatagtcaa ttgctgcctt accaacttca aaactcataa
     5461 gttctttttc tcctccaaaa tcaccttgag ctgcaaaaca gtatttacat tttaagttac
     5521 aatcatgagc tacatttaaa cataaagctt ttactacagg ctctctatgt acaaagcttg
     5581 gatgatattg atatgtatct tctgtatata aaagaccctc tttttccaaa tttaatattt
     5641 cttcatatgc ttcatttatt ttttcttctt gatattttga ttttaatatc tctaatattt
     5701 cttctttact tttttcttta taaaaatcta acaaatcgta tgcaacatca tctaacacat
     5761 gaacagctcc accattcaca tctaaaacga tattatatcc attcatagaa aatttatgta
     5821 tcatactcat accctacctc ctaaattact atctatacta aagtttttta ttattttaat
     5881 taaactttac ccttcattat atcataagtt tacataaaat acatacaata caaaaatgac
     5941 ttactctccg taagtcattt cattaaatac actatatccc cctaaattgg cgcactaata
     6001 tattaccaaa taggtattag gctcctataa aataaataaa ataaattttg tatctgattt
     6061 tatattgttt taatcttatt aaaatataat aatttctttt ttcctgataa ttttgtataa
     6121 ataagtgctt gtatttaatt tgtcttcttt agattgctga tttctttggg tactttaggc
     6181 tgatgaataa tccaaggaca ggtactatta gctgacaaaa tagcagtgct aagtgccaat
     6241 gagctagcaa acttcataaa tcttacaata aactttttca taacttaact ctccctcata
     6301 catattttac ttatacttta gcttttaact tacctaatac taacatcaca aaaatccaaa
     6361 acatggcaaa tgcgctatat gtgaaataat ctacaaatat actcaaagaa agacttaaaa
     6421 ttgttatcaa taacactact gtagatataa atatagcagt tttcttataa accaattttt
     6481 ccctatcact tatagggtta ctcctatgtt ctattgggca cagaacacaa attccaaccc
     6541 aactaacact tgcaataatc ataataatat ttttaaataa gtctatattt atattttcta
     6601 taattaagat tgtagataaa attataaaaa taaaggttaa taagcatcta aaataattgt
     6661 ctgcatgata ccctcctgaa aattgtctta ttggacaata acacattaaa aaaaataata
     6721 catatgtaaa tttaccaaat aaaaaaccta taaacagcat ggtggttata ttaactatga
     6781 aagctattaa tatttcaaat ccataagaat agactttaga ttcattatta tctatcatat
     6841 tattacaaat taaaacagat gtcatttttt cagcatatct tttaaacatt tttacctcca
     6901 aaaaaatttt acatatatag agtacattct tttttgagaa aattcaatat actttacaaa
     6961 actagttgtt aaaaatgtaa aactggtcaa aaaaagacct atataatttt cttcttatta
     7021 tttatataac aaaacaatta taataaaaca
//
LOCUS       DACULQ010000177         6908 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.179, whole genome shotgun sequence.
ACCESSION   DACULQ010000177 DACULQ010000000
VERSION     DACULQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.179"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            145..1926
                     /locus_tag="JA154_15650"
     CDS             145..1926
                     /locus_tag="JA154_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HAT7997264.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(2008..2538)
                     /locus_tag="JA154_15655"
     CDS             complement(2008..2538)
                     /locus_tag="JA154_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAT7997265.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(2578..3402)
                     /locus_tag="JA154_15660"
     CDS             complement(2578..3402)
                     /locus_tag="JA154_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase"
                     /protein_id="HAT7997266.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3659..4567
                     /locus_tag="JA154_15665"
     CDS             3659..4567
                     /locus_tag="JA154_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAT7997267.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            4711..6156
                     /locus_tag="JA154_15670"
     CDS             4711..6156
                     /locus_tag="JA154_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAT7997268.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            6467..>6908
                     /locus_tag="JA154_15675"
     CDS             6467..>6908
                     /locus_tag="JA154_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7997269.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKE"
BASE COUNT         2833 a          828 c         1040 g         2207 t
ORIGIN      
        1 tatacttatt gcaaaatttt tttagcagtt ttgtatttat ttgtatttat tttgaattta
       61 tttactaaat ttgcctataa aaagtatata taaaaatata gttcaaaatg gtataattaa
      121 gtataacaga aggaggtaat gtaagtgagc caatacataa atataaattc tcagttagac
      181 gccctaaaat tgaaggagtt cgagaaaata ttttacgaaa atatcaatga ggacgttaaa
      241 gtagtgccaa aagttactcc ttttaaagga ataaataccg atttgttgta tgttaaagat
      301 ggaagaattt tatttataaa atttatggat acaacagaag atatattttt tattttagaa
      361 gaagagttac ttgaagttat gaatgaggaa tatgaacttt tgaaactaaa aatgggacaa
      421 aagaatagaa atataagtta taactacgtt tacattatgc catacgttga agttgaggaa
      481 acatatgaat ttgaagaatt tgttaataat aatataatag ataaaaataa gcttcaagat
      541 ataatgaata agggttcgct ggaggaatat cttaatgatg agaatgatga aataaactta
      601 aatttatttt tactagacat atgttcagaa tactatataa taaatgataa acttcatcta
      661 aatgaaaaat ttaaaaaaat ttcattctac aatgatgact ataagtatac agcaactatg
      721 atggaagacg ttcaaattaa agatgttgtt tctattaaat atgggaatac acttattgaa
      781 ggtggttctg gtataggcaa aacagcaatt atgctttcta gggctataaa attagcaaaa
      841 gtatatccac atcataaatt agtcatattt acacatacaa aacaattgtg caatgagctt
      901 cgagagagga tagagttatt atacaaagat aataacaatt tagaagttca cacatttagt
      961 tcttttatat ttaaactagc taaaaaattc aatttaattg tagattataa catgttaaaa
     1021 aatgattacg aaaagacctt taataatctt gtaaaacaag cacagaacat tataaaaaat
     1081 aaaaatatgt tcaaggctat ttttattgat gaagcagaaa gctttttaga gtatgaaata
     1141 gattttataa gggagttttt atataaaaca aaattcatat ttaatgtgtg ttcttgtaac
     1201 tccttgaata tctctaatag attgaatata tttaaaaaat tatataatgg tatagaattt
     1261 gatgataaga ttatcttaag taaaaattat agacaagcta aagagattgt agattttaca
     1321 aataaatttt caaataattc taactcatat atcaacgaat taagaccaaa tacagaattt
     1381 agtactttct tttatactaa agcacttaga ggtggaaata agtctgttga tataataaaa
     1441 gtaagtgacc ttgatgacca aataagttct gtaatctggg aaattgagta tttaataagt
     1501 aaaaaaggtc ttgattattc tgaagttgct atcgtttatc cttacaataa gaagaaatta
     1561 aaaagtggaa aaaccattta ctttcaatat atgttaaaaa aagcattgga agaggcaaaa
     1621 attccttata tatgtgcaga agataatttg acaaatataa gtaagaaggt aggaactaca
     1681 atagcaaata tatatgcaat aaagaattta gagtataaag cggtaatagt atgtgaatta
     1741 gagatgttat acaatcaaac tataaatgat attgaacaag attatcaagt aaatgacttt
     1801 gtaggagatt taaataaggt ttatttagct atgagtagag caacagacta cctaagtata
     1861 gtaacaactt ttaatgaaga agcaagtgat ataataagac ttattacaga atcaaaagat
     1921 atttagtaaa aaaatatttg taatttaaga aaataaaaag agactgagat ttgtaaaata
     1981 aactttacca attcagtctc ttatttttta gtctactttc ccaatattat caagaggtaa
     2041 tagtctcacc ataaccttac caagaacttc atcttcatca accataccta catcagggaa
     2101 tctactatca ttactatttt ctctattatc tcccattgca aatagtttac cttttggaac
     2161 aacagtatct atatctccag aagtatagtt attgtgtata tatggttcat ttaataattt
     2221 tccatttaca tacactttag aatttgatat ttttattctg tcaccttcag tagctataac
     2281 tctttttatt aagtcttttt tctttcctgt ttctccatca actaaattgg ttttaaaaac
     2341 tacaatatcg cctttttcag gtttaccaac cttgtatgat atcctattta aaatcagata
     2401 atctttatca tctaaagtag gatacattga ttctccactt acaatagaag gtcttataaa
     2461 ttgtagaatt acaaatgcaa gtacaatagc catagctatt gacttaatcc aatcaaatat
     2521 ttctttttta acactcataa cttttctcct tatcatttgt tattaaatta gaatacatta
     2581 taatagaaag aaataagcaa gaaccactat acccaagact attctataat aaccaaatac
     2641 tttgaagtca tgttttttga tatagtccat aaaaaattta ataactacaa ttgaaacaac
     2701 aaatgctact acagaaccaa cacctaaaat aagccattca aatccagtaa atccaaatcc
     2761 agcttttaga agttttaatg cactagctcc aagcattgta gggatggcta agaagaatga
     2821 aaactctgct gctacatatc tagaagtacc aagtaatact gcacctatta tagtagaacc
     2881 agacctagat gtacctggta taagagctag acactggaat aatcctatac aaagagctaa
     2941 tttatatgta acttgagaaa aatctttata cttaggtttt ttatttctct tttcaagccc
     3001 tatcattatt ataccatata caataagagc aatagcaact actgttgaat taaagaataa
     3061 tctatctata tcatcttcaa acagtattcc aataactcca gatggtataa ctgcaattat
     3121 aaccttaagc cataaacgga ctgtttcttt tttctgctta atattctttg cgctatcaaa
     3181 aggatttaat tttctaaaaa atataacaag tacagctaat attgaaccaa attgaattac
     3241 aactaaaaat gtacttataa atgtatttga aaaattcaat tttataaact catcaactaa
     3301 tatcatatgc cctgtactac ttactggtag ccattctgtt attccctgaa ctatgcccaa
     3361 aatgactgcc ttaaaaattt caagcaaact aaatgtttcc aaatcaaaat acctccaata
     3421 caaatttata gtagcatatt ttattcatag attaataaaa aaatgccacc ttttactaac
     3481 ttataatacc atagtactgt gtagtgcaca atggaagatt gatattttca taaaaccttc
     3541 acaatataac aaaataaact taaaagttat ctattgaata aggggtatat ttgaggggga
     3601 aatatatggt atagtaaaag tatagctatt ttattttatg aaaaggaaag gagttattat
     3661 gtatagtaca aaaatactag atgattatga gtatgaaact tttaatgttt tttctacaaa
     3721 attaaatcca aacacgaaac acgattattt atcaaaggtt atactgttta aagaattttt
     3781 aaaagggaaa gaattgattt atgctacgaa agaagattgt aaaaattttg tagactatat
     3841 acagactaaa tatgcgaaat caacttgtga aaagatatat agttatttac atagttttta
     3901 taacttctta aaaaaagaag gatacataga tataaatcca tttagatatg tagaaaaacc
     3961 cacagtaaca agaataaaaa caaaagatga tgttctaagt atacaagaaa taaataaact
     4021 tataggtatc ctaccaaaat taaatataag agatagagta ataatagtct gtctagttac
     4081 tactgggtgt ttattaaatg aattagttag cttaaagtgg aaagacctta tggtagatga
     4141 aaacgataac tcatatgtaa ggttaggtaa gggaagaaaa gaaagagtag ttaaattaca
     4201 tccatacttt tttaaacttt tagaagatta caggaattat tctgggcttc cagaggtaat
     4261 aatacctagt gatgatttta tttttacaac tcaaaagagt aattctataa cagatagaaa
     4321 tgtcagactt atagtcaaaa aagcattaga tttagctgga ctttctcaat attcagcaag
     4381 agattttaga cattcatttg cagcaataag tttaaggctt ggggcagatg aaagtgatgt
     4441 aaaaaatcag cttggatgga gtgataaata ctatgcaatt agatataagt atgtattaaa
     4501 ttttgttgat agtgaaatag tggattactt aattgaaaaa gataatttgg atataaataa
     4561 aaaatagtaa taaataaaaa atactatata taaatgtgaa caaaaaataa aaaagtcttg
     4621 ttatagttcg taaaattata tataattatt tatgtactaa tgtgtacaca gtaatcacac
     4681 gacaaaagat ttaaggaagg tgctaatata atgatagata ataaatatac tacttactca
     4741 aacccactta cagacagata ttgtagcaag gacatgagtt atatattttc tccacaattt
     4801 aaattctcta catggagaag gttatgggtt gcacttgcag aaggtgaaca tgagttagga
     4861 atcaatataa ctgaagaaca attagatgag ctaagagcta atatagataa tataaatttt
     4921 gatgaagcta gaaaaataga aaaagaagta agacatgatg taatgtctca cgtaaaagct
     4981 tatggtttac aatgtgaaaa agccaaagga ataatacatt taggagcaac atctgcatat
     5041 gttggagata atacagatgt tatacaaatg aatgaagctt taaaacttct aagagtaaag
     5101 cttgtaaatt taataaacaa tttaaaagaa tttgcactta agtacaaaga tatgccagct
     5161 cttggattta cacacttcca agctgctcaa cttacaacag taggaaaaag agcttgttta
     5221 tgggctcaag atttaattct tgatttggaa gatttaaact atagaataga caacatgaag
     5281 ttaagaggag ttaaaggtac tactggtact caagcaagtt ttttaagctt atttgaagga
     5341 gaccacgaaa aagtaaaaga gttagataag aaaatttgta aaaagatggg ataccattct
     5401 tcctatgcag taagtggaca gacatatact agaaagttag attatcaagt aataagcata
     5461 ctatcaggga tagctcaaag tatgcataag atgactaatg atataagact tttacaaagt
     5521 ttaaaagaat tagaagagcc atttgaaaaa aatcaaatag gttcatcagc aatggcttat
     5581 aagaggaatc caatgagaag tgagagaata gcatctttat ctaagtatat aatctctgaa
     5641 tctataagcc ctgcaatggt acaagccact caatggttag agagaacatt agatgactca
     5701 gccaataaga gattggcaat acctcaagca tttatggcag cagatgctat acttgaaata
     5761 gggataaatg taactgatgg tttagtagta tatgaaaata tgataaatag acgtgtaaat
     5821 gaagaacttc cttttatggc tacagaaaca atacttatgg aagctgtaaa aagaggtgga
     5881 gatagacagg aattacatga aataataaga gaatattcta tgaaagctgc ctatagagta
     5941 aagcatgaag gcaaggataa taacttaatt gaacttataa tgaatgatga ttcatttaaa
     6001 atgagcaagg aagaaatact atctataatg gacccaaaaa actttatagg tagagctcca
     6061 gaacaagttg tagaatttgt aaatgaagtt gtagaacctg ctataaaaga ttataaggaa
     6121 gacttaggaa aagtagatgt agacttaaga gtatagaatt gtacagataa aatataatta
     6181 aaaacggcta tcttatttat aaataagata gccattttaa tgggctagcg tcttctctcc
     6241 accgctgtga agagttcttc tttacttact ataccatgaa tttaataaaa tagctatata
     6301 aataatattt aataggaata atataaataa aaataaaaaa atgataagat tactaattaa
     6361 atttaaacat tcagcaaaaa ctattgtact tgcaatattt ttaatgtaaa ctgaaataaa
     6421 gttatttaga taatttaata aattttaaaa caaccgaggt gattatatga actatatagt
     6481 aaaagatacc ataagttttt tagtaaaaac atttcctaag atttattcta gcctgtactt
     6541 agaagaccta aaaaaatttg caccagatta taatgtaaat aaaacacaat taagagcatt
     6601 agttttcata aaaaattatg gagtaataag tatgactgat ttatgctcta agttaaatat
     6661 agaaaaaggc agtttaacta gtatggttga tgatttaact gataagaagt atgtaacaag
     6721 gaaaagagat ttagttgata gaagaaaata tctaattgac attactgaag agggagataa
     6781 gatagcaact gattttatgg ataaattaag tgatggttta ggagaaaaac tttctaaatt
     6841 aactgaagaa gacagaaaaa aatatttaga agcaataaat actcttcaat atatattaaa
     6901 caaagaag
//
LOCUS       DACULQ010000178         6777 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.180, whole genome shotgun sequence.
ACCESSION   DACULQ010000178 DACULQ010000000
VERSION     DACULQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.180"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1350
                     /locus_tag="JA154_15680"
     CDS             <1..1350
                     /locus_tag="JA154_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997270.1"
                     /translation="AMFSIICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIM
                     HFTLKIVFVSFLILSINNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKE
                     EVIKNAKIKGFYKNLVEEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIK
                     INKNYLKVNPIYSNGKEIFNLIDYDDKTINLLVPKKLQVYENEIKKEFRELFYFEKIE
                     VENMYNKELNRALNKTKKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDI
                     DNIDDSFYLSYISRCVYFNSKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINK
                     LEKYLNSEIFTIIIIAISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVS
                     LILTNIIPIGIISTRVDLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            1355..1972
                     /locus_tag="JA154_15685"
     CDS             1355..1972
                     /locus_tag="JA154_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997271.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNKKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            2161..2505
                     /locus_tag="JA154_15690"
     CDS             2161..2505
                     /locus_tag="JA154_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAT7997272.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            3075..3941
                     /locus_tag="JA154_15695"
     CDS             3075..3941
                     /locus_tag="JA154_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAT7997273.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSSELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRIKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            4090..4458
                     /locus_tag="JA154_15700"
     CDS             4090..4458
                     /locus_tag="JA154_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997274.1"
                     /translation="MYDDYKYNKCNKCYNDYEEMTHVHEYSESVKLAEECEDRHNHRA
                     AGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGTDKHIHLICGETT
                     VNDGHCHKFLFTTQIEAPLV"
     gene            4625..5074
                     /locus_tag="JA154_15705"
     CDS             4625..5074
                     /locus_tag="JA154_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7997275.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(5230..5613)
                     /locus_tag="JA154_15710"
     CDS             complement(5230..5613)
                     /locus_tag="JA154_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAT7997276.1"
                     /translation="MVMLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIM
                     HASVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLP
                     HPPPHQNDGAAEVLDRYGYTWYLST"
     gene            5869..>6777
                     /locus_tag="JA154_15715"
     CDS             5869..>6777
                     /locus_tag="JA154_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent protease"
                     /protein_id="HAT7997277.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEF"
BASE COUNT         2744 a          708 c         1078 g         2247 t
ORIGIN      
        1 gctatgtttt cgattatatg tattttgata aatattttga tatctatatt aatagttggt
       61 tttaatagta gaagttctaa atatgtgcta tctctaaatg gaaaaaaatc ttatggtttt
      121 gtaaatataa tgcatttcac attgaaaatt gtatttgtat catttcttat attatccata
      181 aataactgta ttttaaatta taatatgcta caaactcagt taggaaatct atctgagtgg
      241 gataaagcaa aaaatgttta taatcttact cttaaagata caggcgaaca atctttagga
      301 aaggaagaag taattaaaaa tgccaagatt aaaggtttct ataaaaattt ggtagaagaa
      361 aaagatgctt tcttaataga ttcaactaat tttgaaaaac ttgaagatgg aagttacatg
      421 tatgaaataa attcaaaggg taaaaaccca gaagtaagtc aatatggtaa aaatataaaa
      481 ataaataaaa actatcttaa agttaaccct atttactcaa atggcaaaga aatatttaat
      541 ctaatcgatt atgacgataa gactataaat ttattagttc ctaaaaagtt acaagtatat
      601 gaaaatgaga ttaaaaaaga gtttagagaa ttattttatt ttgaaaaaat agaagtagaa
      661 aatatgtaca ataaagagtt aaatagagcg ttaaacaaaa caaagaagtc agatttaaat
      721 gttaatataa tatatgtaga taataatcaa agctatttta cttataactc ccttgttatg
      781 gatgacaata ggaatctcat tgatgaccca atagcaatag ttgacataga taatattgat
      841 gattcttttt atctttctta tatatctaga tgtgtatatt ttaattctaa gaaattagat
      901 gcccttgcag atataagtag cattattgaa gctcaaggtg tagaagcgca tatccaaagt
      961 ttacatgctg tttataatga gtatggttta gaaataaata aactagaaaa atatttgaat
     1021 agtgaaattt ttactataat aataatagca atatcaaact taatgattac ctacaatata
     1081 gtagctagct actatgaaag aaataaatat aagttatata ttaaaaaaat atttggttat
     1141 tcaactacag ctagaagtat gttgttaata gtctcattaa tattaacaaa cattattcca
     1201 ataggcatta tatcaacaag agtagattta agtaataata ttatcttatt tggacttcta
     1261 atattagtta tagaagtaat tgtatctatt gtattagaca agattatctc aaatagttca
     1321 tttaataaaa ttataaaagg agaacattaa aaagatgatt tcattaataa atatatctaa
     1381 aaaatttaaa gataaatcta taatagacaa atttaaccta gaaatttcta agggcgagtt
     1441 tgtagcgata acaggaaata gtggttcagg taagacaact ttacttaata taatgggatt
     1501 attagaaaaa cctaattctg gtgatattat aataaataat ataaaaaatc caaatagtaa
     1561 acagataaga attttacaaa gggattttta tggctatcta tttcaaaatt atgctttaat
     1621 tgagaatgaa aatgttgaaa ataacttgaa aattgcttta aagtatcaaa aaaatatcaa
     1681 ccataataaa aaaataaatt acgcacttga gtctgtaggt cttagaaaat acaataaaaa
     1741 aaagatatac gaattgagtg gaggagaaca acaaagggta gcattagcaa gagtaatatt
     1801 aaaagaatgt gaagtaatat ttgcagatga acctacaggt aatttagata aaaaaaatag
     1861 agacatagta tttgatatac ttaaaagtct aaataagaat ggtaaaacta tcgtatttgt
     1921 aactcatgat ttagagttat cggagcaggc agatagagtt ataaaattgt aataatatca
     1981 actataataa atataagaaa gagattacag ctaaatcaag tttaataata atacgaatag
     2041 aatttaaaca ttaagtaaat tagtagtttg taaatataag tataatatga tatcataaag
     2101 gctcaaaaga aattttatat aataagaaat ctaaaaataa caaatggagg acatataaag
     2161 atggaaaatt taccaaattg cccgaaatgt aattcagaat atacttatga agatggaact
     2221 cttttagttt gtccagaatg ctctcatgag tgggcattag gattgggagc tgaagaagat
     2281 tcaaatatta tcaaagatgt aaatggaaat gtattaaatg atggagattc tgttacagta
     2341 attagagacc ttaaagtaaa aggaagttca tcatctataa agattggaac aaaagtgaaa
     2401 aatatccgtt taatccatga ctcatcagat ggacatgata tagaatgtaa gatagatggt
     2461 tttggagcta tgaagctaaa gtcttctgtt gttaaaaaag cgtaatatta taaataagct
     2521 gtagtgttta ttgtttttca gtacagtata aaaccataag aatctattaa gtaataaaag
     2581 tttgtttatt tatctttata ttttcttatg gttttattaa atataaattt tattgaacac
     2641 caaaatattc tctcctctac aaataagcca atcttattta aattaatata ctttgtacta
     2701 tttatactga aattgtaaat ttaaatgcta tgttatttag gtttgtatag ttaatttata
     2761 tatttctgat tttataaatt gtatttatta ttatatcttg attctaaaat cattaaaaat
     2821 ttcaacttct ataaagattt acaatttatc aaatacatta tgagtatata aacaatttat
     2881 tattacagtg ctacatttaa aataagtctt agtataaagg tattgttaca agtataaaga
     2941 aacttatgta tgctattgga ataaataata ccttaaatta taataaagaa taagaactgt
     3001 taaataaggg acttgattat catttagtta tagataatat aatattaaaa ataaagttta
     3061 agaggagaaa aaatatgata gatatacata aaaatatata tgataataag ttatttgagg
     3121 agctaaagat tgactgtaag aagtgctttg gactttgctg tgtagctctt tatttttctg
     3181 cttcagatgg atttcctatc gataaggaat caggaaaacc ctgtattaat ttacaaccag
     3241 attttaaatg ttctgtacat aatagtctta tgaagcgagg ttttaaaggg tgtactgcat
     3301 atgattgttt tggttcaggt caaaaagtag cacaagtaac atataagggg atagactgga
     3361 tgcaatcatc agaactaaca aatcaaatgt ctgaagtgtt tttaattatg agacaattac
     3421 atgaaatgtt atggtatctt aaagaagcat ctgtattaaa tatatcagat acaatcaaat
     3481 caaaaattga tttaataatt gaagaaactg aaaagataac taatatgagc ccagaacaga
     3541 taattaattt ggatatcata tcacatagaa taaaggttaa tttattatta tcacaagcga
     3601 gtgagtctgt gatgggaaag gttaaatcat ttatcaagac ttctactctt aaaaacatga
     3661 aaaaattaag taaaaatata gatttaatag gagcagacct tagaaaaata aatctaatag
     3721 gagcagattt aagaggtaga tttctaatag cagcaaattt aagaaataca gatttgagtg
     3781 gggcaaattt aataggagca gacttaagag attgtgatat tagaggtgct aatcttgaaa
     3841 atagtatatt tttaacacaa ttacaagtaa atactgcaaa aggagattct agtacgaagt
     3901 taccagcttc actaatacgc cctaaatact gggaaaaata attaaattaa tacataattt
     3961 tacaaaacat gaactttttt gactttaatt catattatac attagagatt ctttacaaga
     4021 atttcttaat ataatatagg gagagatata aaaatgagtt atcaaaatta taaatgtgaa
     4081 agagattatg tgtatgatga ttataaatac aataagtgca acaagtgcta taatgattat
     4141 gaggaaatga cacatgttca tgaatattca gaaagtgtta agttagcaga agaatgtgag
     4201 gatagacata accaccgtgc agcaggggtt actggtgaag ctataccaat aaatggaggt
     4261 acaaatcatg ttcataaaat aaatgataat gtagatttcc ttgaccattt ccataagata
     4321 tgtgttacaa cagggccagc aattagaata cctggaactg ataaacacat tcacttaata
     4381 tgtggagaaa ctactgttaa tgatggtcat tgtcataaat tcctttttac tactcaaata
     4441 gaagcacctt tagtataagc tgatgtgaat tattgtagtg aatttgctag attatagtat
     4501 aataatatat aatagataat atttaaactt aatgaggact atgatattta accttcgtag
     4561 tcctttctaa gttttgtata aaaaagtgtt atgcaattaa aagtgatata gggaggaaat
     4621 ttttatgaaa tctggaataa ttcttgaaat gaaacaaata aattcaacag aatatgacga
     4681 aatgttgcaa gtctgggaat catcagtaag agcaacgcat gattttttaa cagaaaaaga
     4741 tattgaatct ttgataccat tagttgagat tggacttaaa gaagtagaaa atatagtttg
     4801 tataaaagat aatgatataa taaagggctt tataggaata gataaagata aaattgagat
     4861 gctctttata gaagataaat atagaggaaa tggtattggt aaaaaattaa taaaatatgc
     4921 tatagataaa tacaatgtaa aatatgttga tgtaaatgag cagaataaaa aagctgtagg
     4981 attttatatt cacttaggat ttaaagtgtt tgataggtct gaaatagatg gacaaggtaa
     5041 cccatttcca atactacata tgaaattaat ttaaagttat acttctcatt tattcgcaaa
     5101 aatatttact taaattttta tacaaataaa agtcgtgaca gtcttaggac acgacttttt
     5161 tactacacta ttaaattgaa atttatctgt tctatattat gattattaga tgtcgttttt
     5221 ttattttaac tatgtactta aataccaagt atacccatac ctatcaagta cttcagcagc
     5281 gccatcattt tgatgtggtg gtgggtgtgg taaaaattct tgtacaagtg taccaccttc
     5341 ggcaagtttt acagatattt cttctatcgc ttctcgagat ggtagaacaa cacaaatcgc
     5401 catgccagaa attacaacag gcttgtctgt atccgcaccc attatacaac cttcgtattt
     5461 tgtgaaagta acacttgcgt gcataatagg attgtttgga ttgctgtcta atcgcgcaaa
     5521 tatagttttt cctcctccga aagcctcagc ataaaaatta aatgcttttt cacaagtacc
     5581 attaaatatt aaatatggag ttagcatgac caatatatcc tccttgatat agaataattt
     5641 atagttgcac ctgataatct aaattatgat ttagtaaatt gtatcatagg gaaaccttga
     5701 agtatataat tttttattat tatattatac aaatttatta atttaaaaaa atgttatatg
     5761 tacggaaggt ttacaggata aaatataata aaaagattaa ggtgacatta taaaaaatgt
     5821 atattgacat tgacgctacg taattgtata tactttgtct taaaggagat ggtaaatatg
     5881 aatattatag aaattcgctc agataaaata tacaagaaga taatggatgc accaataaac
     5941 aaaaaagaag atatatacag atatgaattg atgaagcctt ttgaatttaa gtggaagtgt
     6001 atgaatgttc caatagttgc tagacagaaa ggtggatatg atgtaattat agcaagtgaa
     6061 atgttagggg ttttatcgcc taaggatatt gatgaaaaac aaaaaaagaa tataaatgtg
     6121 ttatctgctg ataaaatttg ggccacttgt aaagaaacca tagaaaactc tataaatgct
     6181 tttataaaag aagggtatga tttaaatatt aaggactata aatattcaat attattggcg
     6241 aatccaaata gtccttatac aatattaagt gatggatact ggggtgatgg tgggattcct
     6301 ggatatatat ttctatcatt ggttcctaat gaatatacta tcaatagatt accagtatta
     6361 atagcacatg aatgtaatca caatattaga tttcagttta tagagtggaa taataatata
     6421 acattagaag aaatgatgat aaatgaaggt cttgcagaaa attttgcaac atggatgttt
     6481 ggagaggaaa tgttaggacc ttgggtcagt agaacagata tcgaaacatt aaatacttat
     6541 ataaagccaa taataaaaag tgctttaaaa gaaactggat ttcaaaatat aacatcttat
     6601 ctttatggtg atgatatagc taaaatgcaa ggatattttc cagtagggtt gccttattgt
     6661 gcaggatatg cttgtggata ttatatgatt aagtattatt tagaaaagac aaataaatca
     6721 ataatcgaag cgactttatt gccttatagt gagataatcg aagcagtaaa agagttt
//
LOCUS       DACULQ010000179         6761 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.181, whole genome shotgun sequence.
ACCESSION   DACULQ010000179 DACULQ010000000
VERSION     DACULQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.181"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(303..1199)
                     /locus_tag="JA154_15720"
     CDS             complement(303..1199)
                     /locus_tag="JA154_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7997278.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKKNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1340..2767
                     /locus_tag="JA154_15725"
     CDS             1340..2767
                     /locus_tag="JA154_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7997279.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIAKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFNWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2963..5557)
                     /locus_tag="JA154_15730"
     CDS             complement(2963..5557)
                     /locus_tag="JA154_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HAT7997280.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5594..6190)
                     /locus_tag="JA154_15735"
     CDS             complement(5594..6190)
                     /locus_tag="JA154_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997281.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6207..6611)
                     /locus_tag="JA154_15740"
     CDS             complement(6207..6611)
                     /locus_tag="JA154_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="HAT7997282.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         2164 a         1092 c          706 g         2799 t
ORIGIN      
        1 tttagctgtt tacttgctta tttaatagtt ttatttcttt gattaaatat ctttcagaat
       61 ctaagccaga tgtctcaaaa aaactgattt ataatataat cattttttct gctattctta
      121 tttttccaat tttagtttca tattaaaata tacttttcat agacactaca ttctttcata
      181 ctcattttca catttaacta tacaagtagt cttactcatt tatatcctat gctttattaa
      241 tatttattaa gaaaaattca aagatttaaa aatatatatt ttctataaag tatatttatg
      301 aattatttca tatcttcttt taaaataaca ttactaactt ctctatgttc atttctatac
      361 ttagttggag atatattata attcttttta aataagcgac taaaataatt tatgctatca
      421 aagccacatc tcatagcaat ttctgtcata tttaaatctg tttcctttaa ataatatata
      481 gctttatcaa gcctgattcc atttataaaa tcgattgtag tcttacctgt tatttgctta
      541 aatgttctac aaaagtgatg tgcactcaag ttcacacgac tagcaagttc ttttaatgta
      601 attttatcca tatatttttc ttctatcaaa tcaaagatac attcaaattt ttctaaagaa
      661 tactttcttt ttataaattc atcttcagaa tatattttat caatatgact tcttaataag
      721 agtactatta attgatagat attagctttt atagataact catatccaac ttctttattg
      781 tgatattcat caagtatagt tgtaatacat tttaaaatca gagaatcatt ttgaattaaa
      841 ttattaaata caatatgatt taaagccaat ggtgctaagt acttggtttg taacaaatca
      901 acttgattac taaaaaggaa tgtgggttca atacgaatac tatagaattt taaattatct
      961 gataagcttt ctaaataatg taattcatta ctgtttgcta ttacaatatt tccaggcact
     1021 accttaaaat tatccaatcc acattcaaga tatgctatac catcaataaa ataatatatt
     1081 tgtagatgct catgccaatg tcttttaaag tttgctcctt ttttaatgtc atcctttaaa
     1141 aaaatatcta taaagttttc ttgacctttc ctgttaaaat atgtattttt atccttcatt
     1201 attcccccct catcaaaata gtgctaatta taagcaatat agttattgta ttcattattt
     1261 catatcgcta ttatataaaa tgtaactatc attgtaaaca ttaacataat gcagtcatta
     1321 taataaggag gtaataaaaa tgacaaactc ttttaaaaaa gatgataata gaaaaaaaat
     1381 cacagtttta tttgtagtca ttgccatgac ttttatggca acattagatt ctagtattat
     1441 aaatgttgct ctcccagttc ttgcaagcaa actaaatgta tctctagcat ctattgaatg
     1501 ggtaatagca agttactcaa ttattatttg ttcaacacta ttattttttg gaagacttgg
     1561 agatattatt gctaaatcaa gagtatttca agtaggtact attctcttta catcagcttc
     1621 attactatgt ggattaagta attctttaac tttacttata gtttgccgct ttattcaagg
     1681 aataggcgct tctgcctaca tggcaaacaa tcatggaatt atcacagaac tatttccaaa
     1741 agaaagcaga ggaaaagccc tagggatttt agttactgca gttgctattg gaaatatggt
     1801 tggaccatct gtgggaggat ttattttgtc aatatttaat tggaatgtta tattttttat
     1861 aaatattcca atcggtctta ttgtaatctt tttaaataca aaatttcttc ctaatagtaa
     1921 aaaatcttca gaaaatatgg ataaaactgg tgctatactt caatttttag gaacaacatt
     1981 attttttagt gctctaatta gtgctcaaca aacaggatta ctaaatccat atatattaat
     2041 tgcactatta ttatcaatta tttttattat attatttttg atattagaaa aaaaacatcc
     2101 tcaaccgctt cttgaccttg aaatttttag aaattttaaa ttttcattga atttaatttg
     2161 tgctcttaca tcattcattt gtattgcatc atcaagtatt ttaattccat tttatttaca
     2221 aagtactatg aaacttcccc ctattcaagc aggtttattt atgatactat cacctttgat
     2281 tttagccatt ttttcaccta tatttgggaa tatatcagat aaaattaagt cagaaaaaat
     2341 tattttaatt ggtcttctcg ttatgagttt tggattcttt ctaatgtcaa gattgaaaga
     2401 aagttctgcc cttattttat ttgtaattta tatatcgata atatcaatag gacaagctat
     2461 atttcaacct gctaacaatg ctttgataat gtcaagctgt tctagaagta agctaggagt
     2521 tgtaggaagc ataaattccc ttgtaagaaa tttaggacaa gttataggta ttactatttc
     2581 tacaacactt ttatataatt ttatgagtat taaagctgga tatagggtca atgattatgt
     2641 gattaataat gataaaatat ttgtatttgg aatgagaaat gtttatatta ttgttacact
     2701 tgtttgctta ataggtgcaa tattaattgg cttttattta tttaaatata ataaaaatga
     2761 acaatagttt ttataattat attcaaatta tgattatatt ataagaaatt tactaaaata
     2821 cttcctttga gttaaattta atttggacat attgacttaa aaatgtttta ttaatttgat
     2881 aaaatatttc ttttcctata gtttataaat aaaaatcagc tgagcaaaat atttagtata
     2941 ttatgctcaa ctgattcttt tattatttaa ctaaaacttc aatattttca tcttttccat
     3001 ctataactat agtacatcca ttatacacat ctccagcaat aagtttcttt gcaattatag
     3061 tttctaatgt atttcctata tatctcttaa gaggtcttgc tccatataca gggtcatatc
     3121 cttctctcac cataacatct tttgctgaat ttgtaacttc aatagtaata tctttttctt
     3181 ttaatctatt ttttaaatct ttcataaata tatctataat tttcttgata ttttcttggt
     3241 ctaaaggctt aaacattata atatcatcta ctctgtttaa aaactcaggt ttaaatctat
     3301 gcttcatttc attcattact aaattgttag tagtctctgt tatatttcct cctgcttcta
     3361 atagataatt acttcctata ttagaagtca ttattataag agtattttta aagtctactg
     3421 tcttaccttt attatcagta agtctaccat catctaatat ctgtaagaac atgttaaata
     3481 catcctcatg tgctttttct atctcatcaa atagtattac agaataagga gcacgtctaa
     3541 ctgcttctgt aagttgacca ccctcttcat atcccacgta ccctggagga ggtccaacta
     3601 gtcttgatac agcatgtttt tccatgtatt cagacatatc tattcttatt atattatctt
     3661 cactatcgaa taaatttcta gcaagagttt ttgcaagttc agttttacca acacctgtag
     3721 ggcctaagaa aataaatgaa ccaattggtt ttctttcatc ttttaatcct gctcttgcac
     3781 gtattactgc atttgaaact gctgtaactg cttcatcttg tccaataact cttttatgaa
     3841 gttcatcttc tagtttaagt aatttttctc tttctccttc aactagttta gttactggta
     3901 tacctgtcca ctttgataca atgctagata tttcttcttc agtaacttct tcttttaaaa
     3961 gagaattttc agaaccatca ttcatatttt cttcgtactg ctttatttgt tcttctaact
     4021 taggtatttc accatattta acctctgctg ctttgttgaa atcatattct ctttcatact
     4081 tttcagcttt acctttagct tcgtctaatt gagcctttaa gtttttaata tctaatattt
     4141 gactcttttc tttttcatat tttgctgtca tttcatcatt tttagatttt aactctgcta
     4201 tttctttttg aatatcatcc aatcttgatt tacttttatc atcattttct ttaagtaatg
     4261 cttctctttc agtttctaat gtaaataact ttcttctaac aacatctaat tctgttggca
     4321 atgaatctat ttcactacga atcatagctc cagcttcatc aattaaatca attgctttat
     4381 ctggtaaaaa tctatcttgt atatatctat gagaaagttt tgctgctgct acaatagcat
     4441 tatcatgtat tcttactcca tgatgtattt caaatctttc ttttaaacct ctaagtatag
     4501 atattgtatc ttcaacagta ggttcttctg caattacagg ctgaaaacgt ctttcaagag
     4561 ccttatcttt ttctatatac tttctatatt catcaaaagt agttgcacca atacagttca
     4621 attcacctct tgcaagcata ggtttaataa gattacctgc atccattgaa ccttctgttt
     4681 taccagctcc aacaatagta tgaatctcat ctataaacag tataattttt ccatcagaac
     4741 tttgaacttc ttttaaaact gcctttaatc tttcttcaaa ttcacctcta tattttgcac
     4801 cagcaattaa agctcccata tcaagtgaga atacaatctt atcttttaaa ccttctggga
     4861 catctcctct tactattctc tctgctaacc cttctacaat tgcagtttta cctacacctg
     4921 gctcaccaat caatacagga ttattttttg ttcttcttga taaaattctt ataatccttc
     4981 ttatttcttc atctctccca attacagggt ctaatttatt tttcttggct aaatctacta
     5041 agtttgttcc atatctagca agtgcatcat atgtgccttc tgggtcttgt gtttctactc
     5101 tttgactacc tctcacttgt gataaaatat ttaaaaagtc attcttattt atattatatt
     5161 gttttaatat tttaccaaca gctgattttg attctgtttc catcattgca agcataacat
     5221 gttctacact tatatatgaa tctttaaatt ctttagaaat actctctgcc tttataagca
     5281 cctcatttat ttttctagta gctgatactc cttgtgagtc agcaccttct ccatatactt
     5341 ttggaagctt atcaatttca aagtttacag tatttctcaa agaatcaatt gatatattca
     5401 ttttttcgat tatatttggt atcaatccat cctcttgatt aataagtgct gagaaaagat
     5461 gaattacatc tacttgttgg ttatgatttt ttactgcttc gttataagcc tcatttaaac
     5521 ttttttgaac tcttaaagtc attttttcta cgtccatata aatccctcca ttttaatatt
     5581 atatccaaat aaattaaatt ctctcattta taattattga ttttctaagc tctaaaagac
     5641 tatcaagtaa attctcgttt ttttgtatat cgaaactcaa tgttaaatta aatgttccat
     5701 ctgaattaaa aaaatcgaat acttcttttg attttgctat taaatacaag tcttcatcta
     5761 taagtctttt ttcacctatt aataaatact gacatttatc tataaaactc tttttaaaat
     5821 caatagtgta tagattttct aaaatattat attgataaat agcaatatta ttaaacactt
     5881 ttaatatgtt tgaatttaca aaattgtaaa tttgattatt gttatacagt gatatttgtt
     5941 cgtatatata ttccctaaat aaaggatgaa tgttttgctt aagttttaaa gtaacttcag
     6001 ctggtatttc aaaataaagc ttccagtact tagagttatt tttttctaaa ataaaagcta
     6061 ctttgttcaa actggtagaa ctaatctcct ttagtgtaca attaaaatta tagtgtctca
     6121 aactagtttt ttcattctca aatacatagt caaaccactg tagaacattt ctatcaagtt
     6181 ttaaactcat ataaaaacct tcttccttat ttactaacct tattaggtct ttttatctga
     6241 ttatattctg ctaataaact ttctttatca gctctactac ctttatatgc tatatgtgga
     6301 tttatcatat actcaccatt tttcactctt tgtaaaaaat ccattttttt cagctccgta
     6361 aacaatgaag ataaagaagt ttttggatat tcaaattcac aagataattc atataatgtt
     6421 tttctaatct tattattttt atccatagag tttattaatt ttaatggaat taaatccctc
     6481 ttagtataca ttccttctac tactttaata taattcttaa aacaaacatt catatactta
     6541 cttttattca taactaaact tttgctctct gtatattcct gatttaaatc cttttttaat
     6601 gtcttatcca aattaaatat ctcttctcac tctccttatc tctaattgat aatataaata
     6661 tattacaaaa aaatatgtat gtcaaaactg aaaatgcaaa aagttcacct tataagtaaa
     6721 cttttattta gctataagct aaactttatt atttttttaa t
//
LOCUS       DACULQ010000180         6716 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.182, whole genome shotgun sequence.
ACCESSION   DACULQ010000180 DACULQ010000000
VERSION     DACULQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.182"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            54..1043
                     /locus_tag="JA154_15745"
     CDS             54..1043
                     /locus_tag="JA154_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAT7997283.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            1115..1855
                     /locus_tag="JA154_15750"
     CDS             1115..1855
                     /locus_tag="JA154_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HAT7997284.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            2071..3222
                     /locus_tag="JA154_15755"
     CDS             2071..3222
                     /locus_tag="JA154_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HAT7997285.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            3247..4002
                     /locus_tag="JA154_15760"
     CDS             3247..4002
                     /locus_tag="JA154_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HAT7997286.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            4194..6581
                     /locus_tag="JA154_15765"
     CDS             4194..6581
                     /locus_tag="JA154_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HAT7997287.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGCTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         2708 a          693 c         1255 g         2060 t
ORIGIN      
        1 aagacttaaa aatctgttga tagtattatt tataagtaca gtaggaggat aaaatgtttg
       61 aaaatttacc taagatagat ttacattgcc acttagatgg aagtgttaga gtagaaacca
      121 tgcttgatat agctattaaa gagaaaatag atttaccttc aaataatatg gatgaaataa
      181 aaaaattagc aaaggtttct tttaattgta cttcactaga tgaatattta gaaaaatttg
      241 atttaccctt aaaagtaatg caatctaagg aaaatttaaa aagaattact tttgaacttt
      301 tggaagatgc aagtagagaa aatgtaaaat atattgaaat tagatttgca ccattacttc
      361 acacccaaaa aggtatgagt gttaaaaata taattgaggg tataattgaa ggaattagag
      421 aagctgaaag tatttatgat ataaaaggca atttaatatt aggttgtatg agaacaatga
      481 cctcaaaaga ggcattatta gtaattgaag aagggaaatc atttgtaaat aaaggtgttg
      541 tagcagtaga cttgtgtggt ccagagaaag agggattttg taaggaatat aaagatgttt
      601 ttaaattagc aagagaatat ggatataaag ttacaataca tgcaggagaa gctgcaagtg
      661 gagaaaatgt attggatgct ataaatattt taaaggcgga taggattggt catggggtaa
      721 aaattaaaga ccataaaaag gcgtataatt tagttaaaga taaaaaaata ttgcttgaat
      781 tatgtccaac tagtaatgta caaactaaga cagtagattc ttatgaagca catccatttt
      841 atacttttta taaagataat ttacatgtaa gtataaatac tgataataga actgtatcag
      901 atataaattt aagttcagaa ctaaatgtta tatttgatac ttttaaacta ggattagaag
      961 actataaaat aatttataga aatgcagtag aagctagttt tgcagataaa gaaactaaag
     1021 agtatttgaa tagtttaatt taatagataa aaatatttaa actttaaatt aagctattaa
     1081 attgataaaa atacattgta ttaggagaaa aaatgtgaaa gattatgaag atgttgttta
     1141 tattaaacta gtcaaaaaag aacttgaaga atggaagaaa aatatattaa agaaaccttc
     1201 tatatttgac aaagcatcaa agggtataca gaataaattt aatggagtat tgccagataa
     1261 atatcatgaa ataattactt cttctataaa agtaatgaca aaaaccgtac tgtttggata
     1321 taaatatgct acaaagccac cacttagaaa tatgtcatta gaagaaagag aaaaattagt
     1381 tttagaaaaa gctaaaactt acaagactac agcaatgatt gaaggggcag gaacaggggc
     1441 aggaggaatc ctcttaggtt tatctgattt tcctttgctt ttaggtatta aagtgaaatt
     1501 tttatatgat gtagcttcaa tatatggata tgatgttaaa gattatagag aaaggattta
     1561 tatattaaat atattacagt tggctttttc tagtcaacag catataaaca aaacttttaa
     1621 attgatgaat aactgggatg aatatgtaaa tagtctacca gaagacatca atgattttga
     1681 ttggagaaca tttcaacaag agtataggga ttatttagat ttagctaagt ttttacaact
     1741 aatgcctgga ataggagcat ttgtaggatt ttatgtgaat ggaaaattaa ttgataaatt
     1801 atgtgaaact gctgtgtctg cttatagatt gaggattcga gagcttaagt tataagaaaa
     1861 attataacat aaaaaattag ttatacaata tatctatatg tatctaattt tgcattgata
     1921 aaaacaatta tggataaaag gtaaagcatg atagaatcta atgtagaggt ttgtatttta
     1981 atggaaaaat ttagttttta ttgcttaaga tacaaattga attacgaaga gattgaatta
     2041 tgaagagaag taattgggag gaatattttc atgacaaatt tgccaaaaca atttgatagc
     2101 ttcaatgaag ctagacaaaa aggatttata aatgcaaagg aacttaagga aagtgggaaa
     2161 aagatggttg gagttttctg tacatttact ccagttgaaa ttccaatggc agctggagca
     2221 actgtagtag gtgtatgcgg cgttagtgaa gagccaatac ctgatgcaga aagggtgctt
     2281 ccaagaaatc tttgtccttt aattaaatca agctatggac atgcaataac agatacctgt
     2341 ccttatttct atttttctga tttgttgata ggagaaacca cttgtgatgg taagaaaaaa
     2401 atgtatgaag aacttgcaaa agttaagcca acatatgtaa tgcatttgcc aaatacttca
     2461 aaaggagaat ttgcctataa actttggaaa gatgaaatga taagattaaa agaagaggtt
     2521 gagaaatctt taggtgtaac tataactgaa gaagatataa gaactgctat aaaagataaa
     2581 aatgaagaaa gagaactttt aaaagagttt tatgcacttg gtaaattaca accatctgca
     2641 cttacaggcc ttgagttaca taatgtttta tatcaagcag gttttaaatt tgaccgtgca
     2701 gaacttaaac attctttaag aaaagttata gatgatatga aagaaagata tgaaaaaggt
     2761 gaatgtcctg ttcaaaaaga taagccaaga atattgataa caggttctcc tattggtgga
     2821 ataagcgaaa aaatagttaa aactttagaa gatgcagggg catcagtcgt tgcatatgaa
     2881 ctttgtggag ctattagatc aaacgattta ttggttgatg aagaaataga agatgtatat
     2941 gatgctttaa cacaaaaata tataaatata ggttgttctt gtatgatgaa taatgacaat
     3001 cgtattgaat tattagatag aataatagat gaatataatg tagatgctgt aatagatgta
     3061 gttttacaag catgtcatac atttaatata gaaagttata gaataagaga atttgttaca
     3121 aaagaaaaaa ataagccatt tatgtctctt gaaactgatt attcaaaatc agatactgaa
     3181 caattaagaa ctagatttga agcgtttgta gagatgttgt aggaataaat aaaagtagga
     3241 ggaactatgt ttagtatagg agttgactca gggtcagtgg caacaaaggg tgtgctattt
     3301 gatggtgaaa aaattattaa gaaaataatt ataccaactg gatggagccc taaaagtaca
     3361 tctaaacagg tctatgagtt gctaagtagt gaaattgaca aaaaagatat aaaaaaggtg
     3421 gtaggtacag gttatggaag gggagtaatg gattttgctg ataagaaagt aacagaaata
     3481 acttgtcata ctagaggtat ttacttttta aacaagaaca taaggactat tttagatgta
     3541 ggtggtcaag atagtaaagt tataaattta gatagagatg gaaatgtatt taactttata
     3601 atgaatgata agtgtgctgc aggaactggt cgatttttag agattacttc taacctttta
     3661 ggaagcgata ttgaaagtat agatacatta gctaaaggat atgaaccagt taatatatct
     3721 agtatgtgta cagtttttgc tgagtctgaa attgtaagtc ttctagctca aaacatttct
     3781 actggagagg tagcagcagg gatacttaaa tctattgcaa ataaatccac ctcaatgtta
     3841 gcaagaggtg aggtaataga tgaagttgca tttactggag ggcttgcaaa aagtaaagaa
     3901 ctagtaaaaa tgatagaaga aatactgggt aaaaaaattt ttattgcaga agatactcaa
     3961 ataataggag ctttaggagc tgctgtaata ggatttagat aaaaacatat aaaaagaaaa
     4021 tgagaagctg aatttattat aaataaattc agttttttat tttattttat ttatttataa
     4081 aggaaaactt tggtataatt gatttaaatg attgtatatt tgtaaatact tgatatttag
     4141 tcatatataa cattataatt ttaaaaatta gctcaaaata agggggagaa agcgtgaaaa
     4201 aatttacttc aaaaaaagta acaaggtttg ttgcgctgtt tttaatatct attttagtaa
     4261 tgtcaacaat tcctgtatca gctgacaata gcaatacatt gaatcagata aaaaaagcag
     4321 aatacagtga gacgtataaa caatatctaa atgatgctaa aaatggatgt acagaaaaat
     4381 ataatgggat tataccaaat ccatatcagt tggaaggtac tcaaatccaa agtaaaggaa
     4441 gaacagctcc aacatcttat gaccctagaa aattaggtct tatgacatca ataaaaaatc
     4501 aagaggattt aggaatctgt tgggattttg ctgcaatggc aaccttagaa agtttcttaa
     4561 agttaaataa ttatggagac tatgatttat cagaagaaca cttaagatgg tgggcttcag
     4621 atggtgaata tggatggtct gtaaatgata tgaatggtgc tttaaattat gaagctatgg
     4681 gatatttaac atcttggtca ggtcctaaat tagaaaaaga tatcccatac aatggtagag
     4741 tatctaaagc acaaggagca aaaaaaccaa ctaatatgaa tacagcacct actgttttta
     4801 atgtgacaga tgctgtgtgt gtaagcaatg acataaattc tacaaaaaat gctatattac
     4861 agtatggagc tgtaacatct ggttattatg aagatattaa atatcaaagt gatgaccaaa
     4921 attcatatta ttgtccaaca aaaagtttta atacaaatca tgctatttca attgtaggtt
     4981 gggatgataa ctattctaaa gataatttta atgcaaatat tagaccatca aaaaatggtg
     5041 cttggttaat aaaaaatagc tggggagatt ataattctga gggtggatat ttctggatat
     5101 cttatgaaga caagacactt atgtctgata tagataattt ttcaatcaaa ggtggtaaaa
     5161 aaccaaatga tgatgagaag atgtatcaac atgactatgc aagtattgta cctttaattt
     5221 caaaaaagat aacagctgca aatgtatttg atttcaatag aggcgatgaa actctaaaat
     5281 ctgtaatgtt cttaactgaa agtataggag ctaagtatga agtatattat gctccagtag
     5341 taaatggtat acctcaagaa aacaatatga aaaaattaaa agaaggtact gcccaatatt
     5401 caggatacat aacagttcca attgattctt ttgatatacc agaaggaaag ggtgcaattg
     5461 tagtcagtat agaggggaaa aatggagagt caactatagg ttctgagtca aatgtaccag
     5521 gatatgatac atttaaagca aaagctaatt taggtgaaag ttatatcata gataatactg
     5581 gaaaattctt tgatataaat agagatagta acttctatcc gtgtaatttt actataaaag
     5641 ctgttacaga aaagtcttct ggtgagagta tcccaaatga gtctttaatt ggtagtgata
     5701 gatatgaaac tgctatcaaa gttagtcaaa atggtttcaa ttcttcagaa aatgtagttt
     5761 tagtaaatgg cagttcaata gtagatgcat tggcagcaac tccttttaca tctgctataa
     5821 attcaccaat attattgaca caaaaagaag ctttaaattc aaaaactaaa gctgagatac
     5881 agagattagg tgctaagaaa gtatacctaa taggtggaga aaattctata agtaaagaaa
     5941 ttgaacaaca attaaaaagc ttaaatatat cgatagagag aatatcagga agtgatagat
     6001 acaagacaag tttattatta gctcaaaagt taaatggtat aaaaaatgta tctcaaatag
     6061 cagttgtaaa tggagtgaaa ggattagctg atgcaattag tgtaggcgct gcagcagcag
     6121 aaaataacat accaatcata cttgcaaatg aaaagagcga attgcaagga gctgatgaat
     6181 ttctaaattc attaaatata gagaaatctt atattattgg aggaacagca tccctatcaa
     6241 acaatctaga aagtaagtta aaaaatccaa ctagattatc tggtagtagt agagacgaaa
     6301 ctaattcaaa gataatagat aatttttata aaaaagatac tttaaaaaat gcatttgtag
     6361 ttaaaaatgg aatcaagaat caaaatgatt taatagatgg attggcagtt ggtcctttag
     6421 gagcaaaaac tgggtcacca gtaattttag ttggtgataa attagctgat agccaaaaag
     6481 aagttcttaa aaataagact ttagaaaaag taactcaagt tggtggagga gcaaataaaa
     6541 atgcatttaa agagttagta aaattaaaaa gtgctaaata gagtacttaa gtattaacat
     6601 atattagtgt gtattagtaa cttatcttaa taaaagctga aaataattac ttcaaattat
     6661 tgataaataa gataatcaat gattgtaaag ttttgttttc agctttaaat ttatgt
//
LOCUS       DACULQ010000181         6648 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.183, whole genome shotgun sequence.
ACCESSION   DACULQ010000181 DACULQ010000000
VERSION     DACULQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.183"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            449..1141
                     /locus_tag="JA154_15770"
     CDS             449..1141
                     /locus_tag="JA154_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7997288.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1116..1322
                     /locus_tag="JA154_15775"
     CDS             1116..1322
                     /locus_tag="JA154_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997289.1"
                     /translation="MVIRLKNFSGKIIKEKSLIFNCILILVIFTFTQMLGILICFNFF
                     SESLIYSMGIMLFLNFILYKILTM"
     gene            1328..3397
                     /locus_tag="JA154_15780"
     CDS             1328..3397
                     /locus_tag="JA154_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAT7997290.1"
                     /translation="MGNLEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTS
                     TKSGIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSS
                     DSEYDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNV
                     EIINKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQ
                     IFKQATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVI
                     LVSISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSI
                     IVRVILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIF
                     LGFTIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRA
                     IEEQVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKS
                     KRLKLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPN
                     DKVYIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNF
                     DPSEIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYE
                     EEITNME"
     gene            complement(3603..3926)
                     /locus_tag="JA154_15785"
     CDS             complement(3603..3926)
                     /locus_tag="JA154_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAT7997291.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3919..4620)
                     /locus_tag="JA154_15790"
     CDS             complement(3919..4620)
                     /locus_tag="JA154_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAT7997292.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4898..6283)
                     /locus_tag="JA154_15795"
     CDS             complement(4898..6283)
                     /locus_tag="JA154_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAT7997293.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALVEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
BASE COUNT         2593 a          753 c          967 g         2335 t
ORIGIN      
        1 atgtataaaa atagaattta aataagtagc tattattgca gtgaaatgaa tgtttctaag
       61 tatatttggg tggaagaatg gtggaagaaa attagtgaaa tgtaattctt ccaccattct
      121 tccacccaaa aaataaaaac tgttcaataa gaattaatat acatcaattc agctgaatgc
      181 aatcaaaata cttaaatgaa agtaatctga aaacaacata attcaaatca atttattcaa
      241 ataaacctca atttatctta aatcttaaga aatatttaag aattaggata atattttctt
      301 aagatttata ttttattata tagttataag ggaaagttac aaaaatgtaa gccaaagtaa
      361 tataacaatt tagtcattgt tgagaaaggg agtgaaagat attgtataat atagataagt
      421 attattatac aatagaggaa gtgaaaatat gtacaatata ttagtagtag acgatgataa
      481 agaaattgtt gattcgatag aaatatatct taaatcagaa ggatttaaaa tatataaagc
      541 atatgatgga atgaaagctt tggaaataat aacagagaat gacattcatc taatactaat
      601 ggatataatg atgcctaaat tagatggaat caaagcaact gtaaaaatac gagaagaaag
      661 aaatattcca ataattttaa tttcagcaaa aagtgaagat acagataaaa taataggatt
      721 aaatattgga gctgatgatt atataacaaa gccatttaat ctattagagc ttatagctag
      781 ggttaaatca aacttaagac gatatgtaac tttaggtaat tatgaagata catgtaaaga
      841 aattctaaaa agtggagctt tggagttaaa tacactaaca aaagaagtta aaattgatgg
      901 tgaaaacata aaaatgactc caattgagta taaaataatt gaacttcttt tagaaaataa
      961 aggacgagtt ttctcaatag atgagatata cgaaaaagta tggaatgagg aaagttttaa
     1021 ttcagaaaat acagtagcag tacatatcag acgaataaga gaaaagattg aaataaatcc
     1081 aaaagaacca agatttttaa aggtggtgtg gggagttggt tataagattg aaaaatttta
     1141 gtggaaaaat tataaaagaa aagagtttaa tatttaattg cattttgatt ttagtcattt
     1201 ttacatttac acaaatgtta ggaattttaa tatgttttaa ttttttctct gagagtttaa
     1261 tttactctat ggggattatg cttttcttaa actttatttt atataaaata ctgacaatgt
     1321 agatgaggtg ggcaacttgg agaaatatag agtattaaag ttagtttcat taatcataat
     1381 atctatctca tttccattaa ctatattgtt ggtgacaata ggacttgctg agcctaaata
     1441 caatatacaa aaaacttcta caaagagtgg gatttttgat atatctgatg atttttatga
     1501 tagtaatata tttgacgaag gaacaatggc aagtctatta cccaagttta agctatctgt
     1561 aggtggtaaa ttaactagta aagaaaaagg taaattccaa caagaaggta aaagtataga
     1621 tttaagtagt agtgattctg agtatgataa aatcatgatt aatgaacaag cttctgaaga
     1681 atatttaagt tatctaaaaa atataaaagt gtatgttatt aatacataca caggagtatt
     1741 ttatacaaat acgtcatatg ctagtttata tgattttaaa caaaaaacta agagctattg
     1801 taatgtagag ataataaata agataggaaa gagctcatat attaaaacaa taaatggaga
     1861 aaaatttgaa ctgaataatc tgagtctgga actaaatgaa gtagatgaag gttttgaggc
     1921 atatgtatct tttccagaag agccaactat agaagatgga atagtatata ctaattttca
     1981 aatatttaaa caagctacag aaaaagtaag attatttttt gtagctagtt taagtttagc
     2041 aattatatgt ttattatcta taattttata tatcaaaata aaaggtgaag ttttaaaaag
     2101 aaataatgta tttttactaa tatacagtaa gatacctatg gaatttaatg taatattagt
     2161 atctatttca attgcaattt taactatata tgctcttgct gtatcttatc atattgatgt
     2221 aattgcatta gtttctttat ctttactgat agtatcaaat aatatatttt ttataaatgt
     2281 gcaattgagc tatttagaaa ataaaatgtg tatatttaag acaagtatta tagtaagagt
     2341 gattttatgg ttaataaaaa ctgtaaggga ggtcagtaac gctataaaga aagtatcttt
     2401 agcaaggaaa gttattgtta tagtgataat atctttagct ttaatcatgg caattagcac
     2461 tcgaagtata ggtatgggat tactgttttc tatatgtatt tttttaggat ttacaatata
     2521 tataactaaa agactttcat atcttagtta cgtaatagaa ggtacagaaa gaataaaaaa
     2581 gggagaatta gattataaga taaaaattac tgggaatgat aattttacgt ctttagctga
     2641 aaatataaat aatattggag aaggtcttga tagagctata gaagagcaag taaaaagtga
     2701 aagaatgaag tctgacctta taacaaatgt atctcatgat ttgaaaactc cacttacttc
     2761 tataataaat tatgtagatt taattaaaaa agaagaatca atacaaccag agtatataaa
     2821 tgattatata aatgtacttg agtcaaagtc aaaaagacta aaattattga tagaagattt
     2881 atttgaagcg agtagagtaa gtagtggcaa tatagaatta caaattagca agattgattt
     2941 agtgcaacta ttaagacaaa gtataggtga acttgaagaa aaattatcta aaaataattt
     3001 atatttaaaa ttaaatgttc caaatgataa ggtgtatata tgggcagatg gaagaagaat
     3061 gtatagagta tttgaaaatc ttttatctaa tatagcaaaa tattccttag aagaaactag
     3121 agtctatatt gatgtatatg actatggtga aaatgtaaag gtaactatga aaaatatatc
     3181 ttcttatgaa ttaaattttg acccaagtga gattatggaa agatttaaaa gagctgatga
     3241 atcaaggaat actgaaggaa gtggattagg gcttgctatt gcaagagatt tagtagcttt
     3301 acaaggtgga aaattttata tagaaataga tggagattta tttaaagcaa atttagagtt
     3361 tcaaaaatat gaagaagaaa taacaaatat ggaatgagat agtatttaag tatatatatt
     3421 ttttttactg tcgaaagctt ttattataat atattagaat atttacagta gagaatgaag
     3481 agtaaaaaca gagtaacttt aagtcactct gttttttatt taaaatcaat aatataacaa
     3541 cttaaattaa aattgtacac atagttttaa aataacttat acaataaaac attatccaaa
     3601 tatcacaaga taattccaaa taaatatata gatatcactc ctgtaataat agaaatacca
     3661 atagatttaa acttatatgc aacaagaagt acaatcataa aagatataat tttagggtta
     3721 gctatagata aatatagact atctccatta aaaaatacat ctttagcagt taaggaagta
     3781 agtattgcta caggtataaa tttcatgatt tttttcaaaa aatcaggcat ctcttttttt
     3841 gaaaaaatta acataggaag cactcttggt agatatgtaa caatgtacat tccaattata
     3901 ataaaaaata tataattatt attcataaga ttcctccttt ttacagtaag attcttctat
     3961 aaagaaacca attaaagatg ctaaaattga tgcaactatt attactaaat tgcttttgaa
     4021 taatgcatat aaaataacag aaataatgat tgatgatata aatactacaa tataaatctt
     4081 attttttatt tgagaaacta ttaaagctat aaacatagaa gtcaatatga agccagaaat
     4141 agaactgtct atagatatac tttctcctaa aaatgctcca ataaaattag ctaaaactga
     4201 tgaaattaag cagaagaaat taagataaat agcatttcta tcagaccaat ttccagcagt
     4261 aaatttttca taattaactg caaaagtttc atctgttata gtatgacaaa atggcaatat
     4321 aaattttgga cttttatttt ttatgtacat gttcaaatta gaactcatta gaaaatgtct
     4381 taagttaact ataaatgtag tcaaaacaat agatgttaca cttgcaccag tagaaatcat
     4441 tgaagctgca atgaattgag aacttcctcc aaatactaaa gttaccatta gtgctatttg
     4501 aaaaggtgaa agaccagatt tttgtaatag tattcctcct actataccta taggtatata
     4561 acctaagaat ataggaattg aagcaataaa gctttctttt aattgttgag ttctttccac
     4621 aataaatcac tccttttgta ttaaatgtat taaagtatta gtaaaacaga aaaatagatt
     4681 aattgtataa ttgcaattat aattaaaaat aaatttggat taatatataa aatactatac
     4741 aacaaataga taatcttaaa cggttattaa attttatcta tttgttatta tgatgtaata
     4801 atacatatta atctctataa gttaattact atctggtgta attttatttt aacaacaaaa
     4861 aaaaatacac acaattaaaa tattgtgtgc aatacaatta ttttttctct tctaatagtc
     4921 tttttaatac gctcaaacca gctttaagtt cttctgtact ttctgtagca catacagcta
     4981 ttcttattgc agaaattggt ttttcttttc caacagcaaa acgttcagaa gcatagactt
     5041 gtacaccttc tttaagtgct tgtatctcaa atttttctgc cattattcca tcaggtagaa
     5101 tcaaccatct aaatatacat tcttcatttc ccaaaagatt atatccagat aaatattgat
     5161 taataatctt gtttcttctt cttgcagatt tacggcgtgc attaattaat ttatcggctt
     5221 cacctgatgt aatcatgcga taagcaattt ctgttaaaaa ataagattga gatagattaa
     5281 tgttgtataa tgcgcttgat aatgcctctc gatactgttt aggtgtagag atatatgcta
     5341 gccgtaaact aggtgcaata atttttgata aactcgtaat ataaattgtt tgatttggca
     5401 gataggaagc cactggattt aaatgtgttt cattaagtaa actatgaata gcatcctcaa
     5461 taacaattaa attgtattta gatgcaatat ttgcaatcat ttttcgtcca ttttgtgaca
     5521 ttatatgtgt agttggattt tgataatctg gtattatgta tagaccttta atattttcat
     5581 ttttacaagc atataatagt ccttcttcac taatctcatt atcttcttgt ttaattggaa
     5641 ttagttgaat cccaagcatt ttagctgctg tctttattcc aggataagtt aatgggtcta
     5701 cacctattct atctccatgt tgaaacaaac cagctaaaat agcaacaatt gcattttgtc
     5761 caccacttgc aggaagtaag ctatctgatg tagtttcaag cccagcttta taaattaatt
     5821 tggcaatagc ttctttttgc catttagcac catctctttg tccatattgg aatatagttt
     5881 taaaatctat ttccttaatt atgtgtttaa acaaagtatt catttcttct agcgtgaaat
     5941 ctggattcat tgtgcccatt tcaataaatg taattttatt attactggac atcaagaata
     6001 gatttgaacg tgtatcataa gatacaaaag ttccacttcc tgtaactcca cttattagac
     6061 cttttttttc acaaatttta aaagctctag agatagtact cacattaaca tctaagaaat
     6121 ctgccaattc acgttggggt ggtaatttag tacctggtaa taaaaatcca ttgttgatat
     6181 cttgttctag ttgttctaca agcgccttat ataatatttg cccttttgaa ggtctctttg
     6241 gtttccagtt cataggataa ttttcaaaag aattaattgg cataagtttt cacctcacat
     6301 tattaatttg attttttctt tatctaaaac aataaattct aaagatacaa ataaaaactg
     6361 ataggaaata attctatcag tttttgtctt ttaattgatt tcaaatcttc ctataatttt
     6421 tataccttct ttatcataaa gagggattgt ttttttaaat atattaaaat ttctttttct
     6481 tgtatataaa acagcttctt ctggtgtctt tggttggtta tcacaatcgt acaaatttct
     6541 tttttttaac taaaagcaat aatacataaa attattattg ctaatgatac tcttaatgtg
     6601 gtatatagtc atagtgtcac atgtctatca atagtttttt cagtaatt
//
LOCUS       DACULQ010000182         6499 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.184, whole genome shotgun sequence.
ACCESSION   DACULQ010000182 DACULQ010000000
VERSION     DACULQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.184"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..534
                     /locus_tag="JA154_15800"
     CDS             <1..534
                     /locus_tag="JA154_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT7997294.1"
                     /translation="YSNKPQFISNLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVAR
                     LQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRSISDVSWSEFIRQ
                     LEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWIYPSCNETHDRDINA
                     SINILKEGLRLITIQNK"
     gene            658..825
                     /locus_tag="JA154_15805"
     CDS             658..825
                     /locus_tag="JA154_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997295.1"
                     /translation="MTHYCIQKLKWKPSEYMNLEVNERALAADSILIKIEDEEETMKE
                     AEREKKRGRRR"
     gene            966..1220
                     /locus_tag="JA154_15810"
     CDS             966..1220
                     /locus_tag="JA154_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997296.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1247..1579
                     /locus_tag="JA154_15815"
     CDS             1247..1579
                     /locus_tag="JA154_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997297.1"
                     /translation="MNDMARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSI
                     IVTIFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVK
                     KIFIDYII"
     gene            1853..2005
                     /locus_tag="JA154_15820"
     CDS             1853..2005
                     /locus_tag="JA154_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997298.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2199..3122
                     /locus_tag="JA154_15825"
     CDS             2199..3122
                     /locus_tag="JA154_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HAT7997299.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVKA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   3389..4069
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3389..3416
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaa"
     gene            4533..4898
                     /locus_tag="JA154_15830"
     CDS             4533..4898
                     /locus_tag="JA154_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997300.1"
                     /translation="MKNELMMFEGKEIEVFEFEGRILFNSKDVVNCLDIKNVNENIRL
                     MNEKQVVKLRNSDISNTDIRKLNNAGENFLTESGVYKLIFKSRKEEAERFQGWISDEV
                     LPSIRQTGAYINNNAIPKS"
     gene            4952..5347
                     /locus_tag="JA154_15835"
     CDS             4952..5347
                     /locus_tag="JA154_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997301.1"
                     /translation="MLKELLDDAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDT
                     KQIASKLKIYSKSNKPAQMAVCEIIKKIDLEDGEVKGVWEINGSWTGTVNKYTKSVID
                     KVRTWIEENNRPTKIAGEKKNYYVFYKIE"
     gene            5399..5560
                     /locus_tag="JA154_15840"
     CDS             5399..5560
                     /locus_tag="JA154_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997302.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            5648..5860
                     /locus_tag="JA154_15845"
                     /pseudo
     CDS             5648..5860
                     /locus_tag="JA154_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902620.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
     gene            complement(5889..6353)
                     /locus_tag="JA154_15850"
     CDS             complement(5889..6353)
                     /locus_tag="JA154_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997303.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKKKILRERKYKKRR"
BASE COUNT         2675 a          620 c         1116 g         2088 t
ORIGIN      
        1 tattcaaata aacctcaatt tatctcaaat cttaagaagt cattaaacaa acttgctaaa
       61 ttacaaagag aattatcaag aaaaacaatc ggtagtttaa atagaaataa agcaagatta
      121 aaagttgcaa gacttcaaga acatatagct aatcaaagaa atgattttct acaaaaacta
      181 tctactaaat taataaaaga gaacgatatt atttgcatag aagatttaca agtaaagaat
      241 atgattagga atcgtaaact atctcgctca atttctgatg tgtcatggtc agagtttatt
      301 cgtcaattag aatacaaggc taattggcat ggtagacaaa ttgtaaaggt gggtaaattc
      361 tttgcaagtt cacaaatatg caataaatgt gggtataaaa acgaggaagt gaaggactta
      421 aatataagag aatggattta tcctagttgt aatgaaactc acgatagaga tataaatgca
      481 agtataaata tattaaaaga aggattaaga ctaataacaa ttcaaaataa ataacgtatg
      541 taagaaccgt aggaactacg gggatagcct gtggagaatt agtaagacat atgttagtat
      601 gcaaaattct gtgaagcagg aaacctgtga ctttagtcat gggaggttca ggctagtatt
      661 actcattatt gtattcaaaa gctaaagtgg aaacctagtg aatatatgaa tttagaagtt
      721 aatgagagag cattagcagc cgactcaata cttattaaaa ttgaagatga agaagaaaca
      781 atgaaagaag ctgaaagaga gaaaaagaga ggtagaagaa ggtagaattt aaagttaaaa
      841 tagttacatt gctaatatat gaaaatgtta caaatttgga aatagatgga tatatatgtt
      901 gtatagtata atgtagataa tatatcacca agtaaaaaac aacaaataaa tctaaattat
      961 gaggggtgga aaataaagat attaaactta caatatgtat atttgctttt gtaattggaa
     1021 taatagtgtt attttgctgt ataggtctag gtcaatttaa tatggattgt attataaaac
     1081 aaaatggtgg gtcaatagct accgaacaat acaacatata tttagaacaa tctatttcac
     1141 aatatagaaa ttttggttct atactggttt tactaggagg aataggaata ctgtttgata
     1201 aaagtgtcag aaaatactaa aaaagtatat ttttatttag gatgtgatga atgatatggc
     1261 aagagatgta tttagtgtaa agatgacacc actagaagca cagaaaatgg tagaaaaaaa
     1321 tttggaagca gatttagttt ctgttgatat ttataaccta gaaaataata agtctataat
     1381 agttacaatc tttgaaaaat attatgcaag aagcaatagt gatgcaggtt tggtagtgat
     1441 ttgtgaaaat actacaggaa aaacacttgt taaaattacc tcaactggta gtgcaattgg
     1501 actatttcag attgattggg gaacaggtaa taatttgatt aaaagagtaa aaaaaatatt
     1561 tattgattat attatttaat tattttatgt agatattctt aggttggtat ggaatttgta
     1621 ctaacctatt aagtaatcaa taaaactaaa taaacataga aagcacttac aaatatgtag
     1681 gtgcttttta ttgaaaaaat agaaaataaa ataaaaagta agatatagga aaaatatgta
     1741 agaattatat gttataataa ttgtagcaag aagatgtaat ctacaattta tagagtggag
     1801 ttcatgtatt aaaaaattat cctcccaacg ataagaaggg aggtgaatat acatggataa
     1861 ttttttattt aatgtgttag ctagtgtaac agctagttac atagtttatt tgattagcaa
     1921 gttaatcaaa aaagtaaaaa gccactctac cgcaaagagt gacttaaaga ttgatataaa
     1981 ttttaaatat catcgtaaaa aataactttc agttatagaa cttcactcta ccgcaaatag
     2041 attgtagttc ttcttgtttt tattatacca caaattagaa aaaatattgc atataatatt
     2101 ttttatagtc aataaaaata tgaaattttt tatacacaat gatgatgaat ctaactagat
     2161 gaataaattt aattgtaaat aagaaagtac ttacttaaat ggtaggtgct tttgttttgc
     2221 caaattatgc tataatattg agtgagatga gtaatttgtg ggggaatttt ataatgtgga
     2281 tattagtacc aataataaca atagtattac ttataatagc tgtttcttct atgcaatata
     2341 ttttagttat gatagctttt ttgttgatta tatattcttt tatagaaaag aaaatagtta
     2401 tgggtcttgt gtcagttctt tttttcactt attcaattta tctttgtgca acttgggaag
     2461 ataaatcctt gatagcagat aataaagttg agactgttaa ggcacaaaga gaaactgtag
     2521 aaagagaaaa agaaatggaa agaagaagaa tacaagaaga agtagacaaa gaaagatata
     2581 ttgaaaaaca tggaatggaa atatcagaaa atgatttaaa agttaagcta gaggctttag
     2641 tacctcaaga atataagggg aagaaatatg aattaaaagt aggaaaattt aaaagatata
     2701 gtatgtattt tgatttaact gtacaaaatg aaaaatttag taattctgaa gaatgtaaaa
     2761 aatttgtaaa agaaattgct aatgatctta aaaaaattaa aataagcaag gcgtacttta
     2821 agtttcattc aaaagatgac ggcggtatat ataattctgt ttatattgat tattttagaa
     2881 acatacaaaa taatgtagat aatgttgaaa atttagaatt caatgagttt gaacttaaaa
     2941 ccgaagaaga agagaagcga gaacaagaga aaatagaaca agaaaaaaac agttataata
     3001 actacattca aaacagagtg gttgacccac tagatagaat aaagaaacta aaagagttgc
     3061 tagattcagg agcaattaca caggaagaat ataataaaaa gaaaaaagaa ttattagaat
     3121 agataatata gtaagcactt acaagtatgt aggtgctttt aaatttacaa agtattccat
     3181 tttaatttta tagtttagat tttatgatat aataaaaata tagaagtttt gcagtgtgcg
     3241 atatttgtta caaagtaggg cttaatactt gaaatctaag atgttgaggg tgcgtgataa
     3301 gtgttatcaa ttgcactatt gcccgctcac tgcaatttta agagtattgt atatatgtag
     3361 gtattggaaa tgctaagttt attttggggt tttagattaa ctatatggaa tgtaaaactg
     3421 actcgggagc atataataag taatatcaag aatagtttta gattaactat atggaatgta
     3481 aaattcgtaa aataacaatt gtcaggaata tgagaagttt gttttagatt aactatatgg
     3541 aatgtaaaat tggactcaaa atataagtta aaaaaatttt taagttttag attaactata
     3601 tggaatgtaa aaatttttga cgctatgaag aaatttacta caaaatttgt tttagattaa
     3661 ctatatggaa tgtaaaaact tttttcatcc tcccaaacct cttgttcgct gtcgttttag
     3721 attaactata tggaatgtaa aaaagaaaat aagtttaaaa aaaggtttca ttattatatc
     3781 gttttagatt aactatatgg aatgtaaaac caattatgta cagtttagtt actgtgttaa
     3841 gtcttgtttt agattaacta tatggaatgt aaaatccttt tataaatatc ttctgacatg
     3901 tgtatatgct tgttttagat taactatatg gaatgtaaat cggctacaac ggtataaatt
     3961 aaatttagca gggtacattt tagattaact atatggaatg taaattgctg tgatttcaat
     4021 aataaaacaa ttaataattg tgttttagat taactatgtg gattcaaaat taaataaaca
     4081 aagaaagcac ttacaaatat gtaggtgctt ttattctgct caaaattggt cggttgggta
     4141 aaataattag aaaaagttag taaaaaccta ttgactgtaa ctcgttacaa tattgttatt
     4201 aatgtaacga gttacagaaa agaggtgaat aaaatagcaa ctaaaagtag ggcagagtat
     4261 atgaaaaatc gtcgaaaaga taaaagaggt tttagtgtac ttttagacaa agaaaagtta
     4321 gataaatttg atgaagtgtt agaggaaaag aatctaacca agaaagaatg gctagaagaa
     4381 aaaatcgacg aggaactgga acaaaaggaa taaaaaataa gggtcactcc caccgaccaa
     4441 agtttgagta acccctatga cgtatactat cgtatatcaa ttatagtata tgtcattcct
     4501 taaaaaaatc aattattaag gagtgtaata ttatgaaaaa tgaattaatg atgtttgaag
     4561 gaaaagagat tgaagtattt gaatttgaag gaagaatttt attcaactca aaagatgtag
     4621 taaactgttt agacataaaa aatgttaatg agaatataag acttatgaat gaaaagcaag
     4681 ttgtaaagtt aagaaattcg gatatcagta atactgacat ccgaaaactt aataatgctg
     4741 gagaaaattt tcttactgaa agtggagtgt ataaattaat ctttaaatca agaaaagaag
     4801 aagctgagag attccaaggc tggataagtg atgaagtttt accaagcata agacaaactg
     4861 gtgcatacat aaataataat gcaataccga aaagttgaga gaaaaagcaa gtgagattga
     4921 aaaattacag ttggtttata acagtacatc tatgttaaaa gaattgctag atgatgcagg
     4981 ctttgacaat aaatcaaaac tattaacagc taaaacatta tataagaaag caggaattga
     5041 tttaccaata gagataaacg aagaagaaca ttattttgat acaaaacaaa tagcatctaa
     5101 actgaaaata tattctaaga gtaataaacc agctcagatg gcagtttgtg agattattaa
     5161 aaagattgat ttagaagatg gtgaagttaa aggtgtttgg gaaattaatg gttcttggac
     5221 tggtactgta aataaatata caaagagtgt aatagataag gtgagaactt ggatagagga
     5281 aaataataga cctaccaaga ttgcaggtga gaagaagaat tattatgtat tttacaaaat
     5341 tgagtaaatt tatcagttgt attaaataat ttagtttagt tttgggggga ttaatacaat
     5401 gtgtgagaat ttacttgata atatgtatat tgaaaagaga aaagaagaat atagaattaa
     5461 acttttgaaa ataagagaaa cagatataga tatatataat aagttagaaa gtatagtata
     5521 taaactttct gagaaaaaat tagagaaaaa taataaatag acatataaag cacttggata
     5581 ttctactgtt tcaagtgctt tgtttggtat aaaatggtat aatggagata gagaattata
     5641 ttaacccaaa aatggagaaa atagagaaga tgatttagtt gacgctttat cagtaggagt
     5701 acttgcaggt aagacagaat ctctagtact attagttgga aatggaataa gtgatactca
     5761 aaaaagttta ataaataaaa gagaatttag aaatataact caaattggtg gaaatggaaa
     5821 tgagaaagca tttagtgaag tagaaaactt agttaaatag attagaaggt agcaactaga
     5881 gttggttgtt accttctttt tttatacttt ctctctctaa gtattttctt tttcatatct
     5941 tctaatacat ctaatgcagt agtataaaat tgtatattat ttctataagg tatagctatt
     6001 ggaagaatgc ccttgttaac taaaaaaata agaagtacaa taaataaaag tgtcataatc
     6061 attctaataa aataattagt gatatctgat aaatcaaatg ttaagttaat tgttgtagaa
     6121 aatacaacag ccataataga tatatacaaa gcaaaaatgg ggttactatt tttttgtaac
     6181 tgtcctttaa gcctagattg ctctatttct atatcttgaa tactttttat ggaatccatt
     6241 tcactataat acttataaac ttttaaataa tattttttat acttgtttaa agcttcttca
     6301 ttatctttaa ggtctttgtc acttaactct gatttaactt ccccaaaatc cataaaatcc
     6361 ctcctacaat gttatataat atactttaat ttatagcata tttaagaact tatatcagca
     6421 atttgtcgaa cgattttagc taaaaataga gtataatgat aggcttatta tttataagat
     6481 attatttttt atatagtta
//
LOCUS       DACULQ010000183         6487 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.185, whole genome shotgun sequence.
ACCESSION   DACULQ010000183 DACULQ010000000
VERSION     DACULQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.185"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..665)
                     /locus_tag="JA154_15855"
     CDS             complement(<1..665)
                     /locus_tag="JA154_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HAT7997304.1"
                     /translation="MGKVAKILLSLIVIVLVLVGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKV"
     gene            957..2621
                     /locus_tag="JA154_15860"
     CDS             957..2621
                     /locus_tag="JA154_15860"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088573.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAT7997305.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(2728..3627)
                     /locus_tag="JA154_15865"
     CDS             complement(2728..3627)
                     /locus_tag="JA154_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088574.1"
                     /note="similar to YegS from E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase"
                     /protein_id="HAT7997306.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3654..4292)
                     /locus_tag="JA154_15870"
     CDS             complement(3654..4292)
                     /locus_tag="JA154_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HAT7997307.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(4629..5627)
                     /locus_tag="JA154_15875"
     CDS             complement(4629..5627)
                     /locus_tag="JA154_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HAT7997308.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KKISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(5725..6300)
                     /locus_tag="JA154_15880"
     CDS             complement(5725..6300)
                     /locus_tag="JA154_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997309.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         2201 a         1032 c          718 g         2536 t
ORIGIN      
        1 tgaacctttc cagagtcctt taaatatcct aatgccccat ttatttgtga ttttaaatct
       61 atttctactt ccatatccaa atcaacattt ccttttttta tagatatatt tttaagtgtt
      121 attggataaa gataactttt ttccaaagta atcttatttc cattaacctc aaatggaact
      181 tttttattgt agttacttaa tattttttct agcattttat cacttagttt aaattttcca
      241 attttcacat tctcaagact tacaactaag tttccatcct ttaaagtagg tctcaattca
      301 agttcatatt gactatttac caaaccaaat accttatatg gtcctgctac ctttatttta
      361 ccatttttca tttcaacaaa attattttca agttcattaa ttccatgctt ctgcatcaat
      421 gtatatatta aatttttaaa ctcttcctct gaaacagata tttttccaac cattctaagt
      481 ggatttttta ctacctccat agaatttata tatgagcctt ttgataaata ttcttcatct
      541 atgattttat tattttgtga actaacatca tatctttctt ttggtgtcaa gatatataca
      601 acaattccta caagtactaa aacaataaca attaaactta acaaaatctt tgcaaccttt
      661 cccataatcg tctcctttac aaatctgtca tcagatttaa aataaattta gataactaac
      721 ttcacataat attcttaaca atatacatac tataatacag tcttttaaaa attaattcaa
      781 ccaatctaat gttttgtaaa taaactgtaa aaacatgttt ttagtataaa agttaatatt
      841 ttgcataata ctcttaatta cacctttatt tttttctaat cttggattaa aattaatatt
      901 atgcaataca gtgtagtgtt ctgtattata atcaaattaa ataggaggta tagaatatgt
      961 caaatgaaac gaactcatct aactttataa agaatattat tataaacgac ttagaaacag
     1021 gaaaacatga cagtatcata actcgttttc cacctgagcc aaacggatat ttacatattg
     1081 gtcatgcaaa aagtatatgt cttaattttg gattagctaa ggagtttaat ggaaaagcta
     1141 atttaagatt tgatgataca aacccattaa aagaggatgt tgaatatgta gagtccataa
     1201 aagaagatgt aaagtggtta ggatttgatt ggaacgagtt aaattttgca tctaactact
     1261 ttgatgaaat gtataagaga gctttaatac taataaaaaa gggtaaagct tatgtatgtg
     1321 atttgacaca agaggaaatg agagaatatc gtggtactct aactgaacca ggaaaagaaa
     1381 gtcctcatag aaacagaact attgaagaaa accttgattt atttgaaaga atgaaaaatg
     1441 gtgaatttaa agatggtgaa aaaacattaa gagccaaaat agatatgtct tctccaaata
     1501 taaaccttag agacccaatt atatatagaa tatctcattc aacacaccat aatacaggag
     1561 ataaatggtg catatatcct atgtatgctt ttgctcatcc aattgaagat gcaattgaag
     1621 gaataactca ctctatatgt actttagagt ttgaagacca aagacctctt tatgattggt
     1681 ttgttaaaga atgtgagatg gaaaatatac ctagacaaat agaatttgct agacttaata
     1741 taaataatac tgtcatgagt aaaaggaaat taaaacaact tgtagatgag ggtattgttg
     1801 atggttggga tgaccctcgt gttcctacaa tatccggaat cagacgtaag ggatatactg
     1861 cagaagcatt gcgcaatttc tgtagtgaaa taggggtatc aaaagttaac tctacggtag
     1921 atagtcaaat gcttgattat ttcctaagag aaaacttaca accaaaggct cctcttacta
     1981 tgggagtttt aagaccttta aaacttatta taactaatta cccagaagat aagattgaga
     2041 tgcttgaaat agagaataat gctaaagatg aatctcaagg aaagagatta gttcctttct
     2101 ctagagaatt gtatatagaa caagatgatt ttatggaaga acctgttaag aaatatttta
     2161 gatttttccc aggaaatgaa gtccgtttaa aaggtgctta ttttgtaaaa tgtactgatg
     2221 taattaagga tgaaaatgga aatgtagttg aaatacatgg tacttatgac cctgaaacta
     2281 agagtggctc tggctttaca ggacgtaagg taaaatctac aatacattgg gttgatgcta
     2341 aatcagctat cccatgtgaa tttagattat ttgaaccatt aattcttgat gatatacctg
     2401 aaaatgaagg taaacatttc ttagaacaaa taaatcctaa ttcattggaa atattacaag
     2461 gttttgttga acctacacag ataaaagatg ctaaaccttt tgataaattc caatttgtta
     2521 gaaatggatt ctttagtata gataataaat atacaactga tgaaaaattt gtatttaata
     2581 gaatagttcc tctaaaaagt tcttttaaac caggtaaata atcttacaat caaaaaatga
     2641 agacttaaaa tataaattac ttctactata aataaactta gttagaagta attttttatt
     2701 tgagtcttca tcatttactt taaatgatta acttttttca ctacagattc ccatgacttc
     2761 tattccatct tttatgcatt ctatgttaag aggaaaatct ggtccttttt ctccgtcaat
     2821 atctgttata agtgaatctt cacattctat atgcaccttt ttagttttaa agtatagtat
     2881 ttcatttcca ttatattgat ctaaataatc acctctaagc acacttatca aaactggtat
     2941 tgattttggt attggcattc ctttaaaaat aattacatcc aaatatccat catctatttc
     3001 agccttatat gctagattta tatttcctgc tgttttgcca ttaaaaatca acataagata
     3061 catatctcca tcataataca tttcctcaga agatactttt atcttaaatt tcctcatata
     3121 caatgcttct tctattccct ttatataata agaaattctt ccaaatgaat tttttaagtc
     3181 tgtgtttatc ctttgtgata catccgtaaa cataccagca cttgcaacat ttataaagta
     3241 cttattattg actttcccta tgtctatttt ctttggtttt gaatttatga tttcatttat
     3301 agcttctttt atatcaaatg aaatttgaag agcatttgaa aaatcatttg cagttcctgt
     3361 aggcaaaatt ccaattggaa tttttatgtc taattctttc attgcattta ctactaaatc
     3421 tacagtacca tcaccaccag ctatcaatat gtgttcataa gaactatcaa cttccttaaa
     3481 tgcattcttt acaggttctt taatagttaa tctatagggt atcaataaat aatttttttc
     3541 ttgatataat tctatgatag tgtctaaatt tgataatatc tttttttcac ctgaattagg
     3601 attgtatata agttttactt ttttcattat ttctccttac tcattttatt cttttagtat
     3661 ccaaaatctt catttaaaaa aatcacatgt attctttctg gtgttaattg tctttttata
     3721 agtgtataac tacagcatat tcttcctgga cttgaacagt ctatacatat accagtagtc
     3781 ttacatccag tatttgtact taatctttta caattggctg gagcagctat tgtttttgtt
     3841 ctttttatag cttcatctac atctttaact attttattta ctcctgctat aactattact
     3901 ttatcaggtc catatatcat agcagcaact ctattcccat tcccatctaa attgtaaagt
     3961 tctccatcct ctgttatcgc attagtactt gtaaaataag catctgcaga aaatgacttt
     4021 ctatatattt ctttcatttc ttctggtttg atatcttttg catatctatc aagaaaatta
     4081 tatttttctc ttataaaatc aatcactcct gtttcaaata aggtcataga acctccacat
     4141 cctactagag aacctgcttt aactatttct tcaatttttt taagaagctc ttctttgtta
     4201 cttactaaat accctttcat attattgttt tccaaagatt ttattgtttt ttctattctt
     4261 ttttcattaa gccaatttaa attttgatcc attttaactt cccccttatt tttaatatgt
     4321 acttaattat ttataaactt tattttcttt ataagtttat tttagtacat ctaaaaaaaa
     4381 atacaatata aaacttaaat agttttacat aattttttcc aaaataactt actcaatcaa
     4441 aaaaggaaag tagatgtact attctacttt ccctatattg tatgtagtat ttattgccta
     4501 aaatctatat attaattata tgtttcatat tatataatat cacagttaat taaaatgtaa
     4561 tactatcttc tttttaaaca tataaattgt attattaaat ttaatatatc caacttcaat
     4621 aaatcttatt aatttgatga agaagatgct atagctgcac ttgttgctgc cattacacat
     4681 gcaatttgaa tagcaacaac aatatttaaa aatatattat tagttataga gcctatatta
     4741 ttctcatttc gtatcaaatc tgcataaatt gaagctacaa tagcagaact aatcattact
     4801 ctattacttt ttcctaaaga ccaatttcca aagccttttt cttctttaag tttatttgaa
     4861 acatttttta tctcttctat tatttcatct tctctataat ctaataatga aattgctcct
     4921 attattggat atccataata attcatttta caatcatttt ctttaaatgc ttttttaagt
     4981 tcaatagtct ttttacattt aagcatagtt ctatcatcac taaataaaag aatttgggat
     5041 aatgcttgta aatcatttcc tttataaaaa ccattttcat ttagaaattc ataacatttt
     5101 tctatgtatt ctaaatcttc atctaaattt tttgaatgaa ttgcaataag tgctatatta
     5161 caatagtcat cactagaagt tagaaatgga tgttttttcc tcatgtaatc atatattatt
     5221 ttcatttttg atatacatgt aagataatcc attttctctc tattttcata aactattgat
     5281 gctgtaagtg gtaaatatgt atcgccccaa aacttttcac ttttaagttc tttgtatata
     5341 tctaaaatca tcttaaaact actttctgga tttgattcaa acgaaagtag tgttgccata
     5401 taaaacatat tatatcctct aaaattagag aataatccag tatttttttt aataagctct
     5461 attcgttctt ccactaatct aggacttatt ttcttgttct taagggtaaa tgcaagtgca
     5521 caactatgtt gtatcaatcc catttgccaa gaacctttaa cttcatttaa tgctttgtaa
     5581 ttttctatta ataagtttac ttttccattt ataaacgatt gttccatatt gccctcctag
     5641 taatatatct cattgtacta atagttttat atttattata tatatattaa atactacaat
     5701 caatacatat tacttcaaag aacatcattc ttcaacaata atgtagcctt ttttgtaatg
     5761 tcttcccata ttattgactc tatcatagta ctctgattca ctttcattac tccatttggg
     5821 cacaaataca tctattgcat tagaattact aaaatactta ctagaccccc tatccataac
     5881 agtttttaat tttccatcta caataatctt agttccaaaa cttaagtgat tgtttgctac
     5941 aaaaccatct tctaaatcat ctcctgtagc agttatcgct ccatatccat tttcttgatt
     6001 aagacttgtg taataactaa cttcaaaagt tataggtact aacttcttta ttttaatacc
     6061 taactgattt tgtagtttct ccttttgtac tttcaactta tactcatcac tatttttata
     6121 atcttccata ccttgattat acttggaatt aaagctttta cctgacttta ctatagtatc
     6181 tatatttact atagattctt tacaatgatc tgcatctatt tttgtttctc catttaccaa
     6241 taatggagtt aaacatacac tacatacaac acatgtaatc aatgcttctt taagcttcat
     6301 aaattccccc cttattattt tgatatatta gcatatttaa tattatatgt ctacatttag
     6361 atttaaatac cacacccatt tgctttaaga agttgtctta tatttaattc accttaaaca
     6421 cttataaatc ctctatctta gaatcatata gaataaagct tttatcatta aatatttttt
     6481 attaatc
//
LOCUS       DACULQ010000184         6447 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.186, whole genome shotgun sequence.
ACCESSION   DACULQ010000184 DACULQ010000000
VERSION     DACULQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.186"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            62..1804
                     /locus_tag="JA154_15885"
     CDS             62..1804
                     /locus_tag="JA154_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HAT7997310.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(1968..2162)
                     /locus_tag="JA154_15890"
     CDS             complement(1968..2162)
                     /locus_tag="JA154_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997311.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2159..2740)
                     /locus_tag="JA154_15895"
     CDS             complement(2159..2740)
                     /locus_tag="JA154_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7997312.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2908..3726
                     /locus_tag="JA154_15900"
     CDS             2908..3726
                     /locus_tag="JA154_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7997313.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQVFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4401..5222
                     /locus_tag="JA154_15905"
     CDS             4401..5222
                     /locus_tag="JA154_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HAT7997314.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGNVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEIWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(5532..6311)
                     /locus_tag="JA154_15910"
     CDS             complement(5532..6311)
                     /locus_tag="JA154_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HAT7997315.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2424 a          878 c          822 g         2323 t
ORIGIN      
        1 cattttttat atttaatata gtaaaataat taataactat atccatgatt ggaggatttt
       61 tatggttaaa tccaagaaga aaatatttgt ttttctaatt ttaattgcag tttttattat
      121 atttgcatca tacataagaa attatactaa tgatacaaaa aaagattctg ttagaggaac
      181 tattcctttt gaaaaaatgg aatataagcg tcctaatata cagtcaattt gcgaaaacat
      241 taacaagtgt aacgacaaac ttttaacagc taaaacagct aaggaacaat taaatttatt
      301 taatgaagtt gataaaattt atcaggattt ttacagtaca cttactatag caaaaattag
      361 aaataacata gattcatcag acgaatttta ctctaaggaa tataagtatc ttatgagtaa
      421 atcagtagat gttgacatgt catacaaaga ctttcaagat agatttgtaa cttctaaatt
      481 ctctaaagaa ttagaaaaag aacttggaaa ggatacattt aattatctaa agaacattgg
      541 aaaacttaat tctaaagaag ttgaaggttt gcttaaaaaa gagcaagacc tagttgtaaa
      601 atatgaagat ttattagcta aatctacagt atcaatagat ggtatagagg tagactttga
      661 agaagccttg tcaagaccaa atcttagccc tgaagaatat gtgaaaattt attcagatta
      721 cttaaaaaaa tacaatccta tatttggtaa catattctta gaacttatac agacaagaac
      781 tgaaatagca agtaagcaag gatttaagaa ttatatagat tatgcctata tgaacctaaa
      841 caaagactat tctcaagaag aagctaaaaa atttagacaa gatgtaaaag attacatagt
      901 tccattatat agagagattt catctaaacc atctgattct tctatttata ttaaagtata
      961 taaaaatcgc tcatttagga agtttgataa agtacttgaa gatatatctc caaaactaaa
     1021 agagagcttc gactatatga aaaaatatga cttgtacgat tattcttctg gaaaaaacaa
     1081 atcacctgga ggatatacta cttatataaa taaatataaa gcacctttct tgtttaatac
     1141 atgggacaat tcttttttag gtgttactag ttttgctcat gagtttggtc atttttataa
     1201 ttactacaac agtattaact taaataataa gatgcaacca agtatagatg tatgtgaagt
     1261 tcattcaaca tctttagaaa ttctatttta taaatatttt gatgattttt ttggtaaaca
     1321 atctgaagct ataaaaaaag aacacttatc gattgtttta aatactatta ttgatgcttg
     1381 tctttatgat gaatttcaag aaattatata caagaatcca tatatgagtc taaatgatat
     1441 caataaattg ttttttgatt tagaggaaga atatggagtt tccaataata tacttagaga
     1501 aaaaaatgct cctttttgga tacttgtatc tcataacttc caagttcctt tttattactt
     1561 gagttatggt cttgcatctg atgtatcact tcagatatgg caattatcac aagaagatta
     1621 cagaaaagct gttgatgtat atatggattt tttaaatcaa aatactgatg ctggatttaa
     1681 agatgtggta gaaaaggtaa atttacaatt acctttccaa aatggaaatt tagaggaaat
     1741 aagctctact ttatatgatt attttggtat agataatcca cttgaactaa aaaatgcttc
     1801 ttaatttcta atactttata ctcactctta agatttagta ttagattgaa ataagtgata
     1861 ttttttcaca cacaattaat gtttaataaa attatatggt aaagtatcag aaatttttaa
     1921 gactcttgtc tttataattt ctgatacttt atttattaat aatattatta tttttttata
     1981 tatataatta aaaggattgt ggaaatcaat aaaaatatcc ctgtaaaaat agcactattc
     2041 atatggaact taatagaact tgcatctgtt accttattat tcttaatcca gaacccacaa
     2101 accatagttg aaagaaaaag taaaacagtg ataagacttg aaccaatagt tataattttc
     2161 atcttacatc aacatcctct ccaataatat tttctattaa aatatcaatg aatttatctc
     2221 gctcatattt attctttata tttataccac tgtataactg aaatgattct gtagataaag
     2281 cagaaatctg tattggtaaa ataatttgca gggcaatgat tcttagactc atttcatctt
     2341 ttttatttcc aaaaatttta cgaagcatag ggacaatatc aagttctgct tgcatatctc
     2401 cattggttat acattgattc aatacaaaag gaagcacttt ttcataatgt aatccagtat
     2461 cacacaattt ttttaataaa ttttttaaat cttcaatagg tcttagagta tatacactat
     2521 cgtcatatag ctctgctatt atatttgaca tcacatctcc tatggcagta ctcaacaaat
     2581 tatcctttgt tttaaaatga taatttatta aaccaactcc aacatttgca cgctcagcaa
     2641 tttttctaac tgtaatctcc tcaatatcat ctacttcatt taaaagattc aatgtaactt
     2701 caattatacg ttctctagca tctttatcat tacttttcat aatagacctc caaaacaatt
     2761 aacattttcg gttctgaaca tgtttagtat aatatttttt tcatcatatg tcaatataat
     2821 ttaattataa ttttagttga ctctaacatt atgatatggt ttatattaat attaagctaa
     2881 aagtaccttt ataaaggaga caaaaacatg tttaagatag gcgatttttc aaagctaagt
     2941 aaaattagta ttcgtatgtt acgtcattat gatgaaatag gacttttaac tccttctcat
     3001 acgaataaaa ctaatggata tagatactat agtgcagacc aactttctac tacaaacaga
     3061 atccatgcac taaaagatat gggctttggc ttgtattcta taaaagaaat tttgacagaa
     3121 tataacgata aagaaagtct cataaaatat ctaaatatcc accattctca agtaaaagaa
     3181 caattagaag atacacagaa aaaactatta aaaatagaaa ccactattaa aagaattgga
     3241 ggaaatgata ttatgaaaaa ttatgatgta actataaaga attttgcacc aaaatatatg
     3301 atgacactta gaagggttat accaacttac caagacgaag gaatgttatg gcatcaagta
     3361 tttttagaga caaaagacca gaatgttcaa attgaacccc caaagtattc taaagcagtt
     3421 ttttatgata ctggatataa agaagattat gtagatgttg aagtacaagt tgctgtatct
     3481 ggaaagtata aagatactga acatgttaaa tttaagactg ttccaagtgt gactgctgca
     3541 actgcaattg ttaatggaaa tttcaatcaa gttgcagacg cttgtgaggc tattggtaat
     3601 tggatatcag ataataacta tgatgtggat ggtcctatgt ttaatattta tcatgtaagc
     3661 cctggcaacg atagtaatcc tgataattgg gtaacagagg tatgcttccc agttaaaaag
     3721 aagtaatttt tatattaatt ggataatatt ttaatttgtt agacaaatta cttttagtgt
     3781 tatgtataaa tacctacaat ttgattagga tattatctta atttgttaaa caaattactt
     3841 ttagtgttat gtataaatat ctacaattta attaggatat tatcttaatt tgtcagataa
     3901 atcaattata agattatata ctatccttat actctagtta aatatatttt aatcactaat
     3961 ataaattagt ttttttaaca aaacaataaa agagtaaagt acaaataatt cctttgtgct
     4021 ctactctttt taactttctt tagacaataa tacaatgata aatataaaaa attatatatt
     4081 ccattctata gaaatatgct caccaataac atgtatgttg ttaatcaata tattatatca
     4141 aattatatca aaattacata aaccttatag gacaatcata tcttattaca agaaaaatgt
     4201 cttatccata ttgaataagt cattcagttc atatacaaag tcaatttcat tgagaaacat
     4261 ttaatatatg tttataaaaa tttcatacta ttctttcata tttacatgtg atataattta
     4321 ttaactcata attttgttaa tcagttgtga ttgtagatta tttatataat ctaatatttt
     4381 aagaaggtgg tgtaataact ttgaaattaa aaattgttgg tagtggcggt atgtcaatta
     4441 ttccaagttc attttgcaaa tgcaaaatat gtgaagaagc cagacaaaaa ggcggaagat
     4501 atgaacgatt agggccaagt ttatatattg atgatataaa aatgctgata gacacacctg
     4561 aagatattgc agtagcttgt aacagacaaa aaatatcgga agtaaaacat atttcaattt
     4621 ctcaccatga cccagaccat gttaagggta ttagaattgt cgaaaaaata ggatgtgact
     4681 ttatcacagg agaaaataat ccaattggct tttatgcgct tcctgaagta attgaagata
     4741 taaaccgaac gaatctcgat tgtcttaaat attatggtaa tgttataaat tgcatttcta
     4801 ttaatgaaac ttcgcatgta aagataaata attttgatat tgatttgatt aacaataacc
     4861 cagacaggaa tataaccttc tatgttatca aagagaatga aaaaaaagtc atatatgcct
     4921 gctgtaatcc caagccattt actcatagtg atatgtactt tgatgctgat gttttaataa
     4981 taagtctagt ttctgatgat ggtatattaa atgatggtac aaaacttaaa gacacaccat
     5041 ttaaagatga aatatttact ttagatgaga ttgttgaaat taaaaactat tatagaatta
     5101 aaaagattat cattacacat attgacgaaa tatggggaaa atcatatggt tattacagtg
     5161 aattagaaaa gaaacttgat aacatttatt ttgcatatga tggtatggaa attatagtct
     5221 aaatcattaa gtaataagca taatgcaaaa taagaaaact tcaatttaac agtatagtta
     5281 aactatgtaa tatcaacata tctcagtttt tacaaaatat catagtattt atctttataa
     5341 aatttcaagt cctacatatt ttaatacttc aatatctaaa taaagtaaaa tcaaaaatat
     5401 attttatata cattatagac ggcttatcgg cactagataa gccgttttgt cacaattatt
     5461 atttgctcaa caaaacttct cataatatat aacagattga atctccaact gatacttaat
     5521 taccgaaact cttattcctt aatatgttgt tgcattaacc aaaccttcat catcattttt
     5581 tgtggcaata gttttgctaa taaatgactg gattttgtat ttataccata cacagaaata
     5641 tccttatttt tctttgcatc cttcaatgct tttttggcaa caatgtctgg agacactatg
     5701 cctgggaaat tttttgtccc tttttcagct acaactatac ctctcttaaa tagatttgta
     5761 ctcatccaac ctggacatac agcaattgca tttatccctt tactcttaag ttcaacattt
     5821 aatgctcttg tataatttct tataaacgct ttagttgaac tataaatatt ttgatatggc
     5881 aatggctgga atgatgcttg tgaagaaata ttcatgatat ggcttccctt ttgcatatat
     5941 ggaatacata taagtcccaa tgctacaaca gcactaatat ttaaatcaat catattaata
     6001 gattcatcta tacttaaatc attgtatgaa cagaattttg caaaaccagc attgtttata
     6061 aggtatttta tacatacatt ttctttactt aaaaatgtac caatttcttt tacttcttct
     6121 atttttgata aatcttttga aaaaattttt accttacttc caaattcatc tactaattga
     6181 tttaatctcg ccttgtttct tgcaactgcc catatttctg ttatttcttc ttcatttatt
     6241 aataacttta gaaactcttt accaaaacca ctattagcac ccgttataat tgcaatcttc
     6301 ttataactca taattattct cctttaccat aagtttatac gctcaaactg taaacttatt
     6361 ttttacaaat aaaacaccct ctattcagat gttatattca tattatttga gatattatat
     6421 ttatatcatt ttcaataatt gttatat
//
LOCUS       DACULQ010000185         6420 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.187, whole genome shotgun sequence.
ACCESSION   DACULQ010000185 DACULQ010000000
VERSION     DACULQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.187"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            100..2601
                     /locus_tag="JA154_15915"
     CDS             100..2601
                     /locus_tag="JA154_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAT7997316.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQNVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2761..3429
                     /locus_tag="JA154_15920"
     CDS             2761..3429
                     /locus_tag="JA154_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7997317.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3413..4690
                     /locus_tag="JA154_15925"
     CDS             3413..4690
                     /locus_tag="JA154_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAT7997318.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5013..5312
                     /locus_tag="JA154_15930"
     CDS             5013..5312
                     /locus_tag="JA154_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997319.1"
                     /translation="MILQINICTLGKINIGSIIFTEEVVLMDRLKRDMKNMKEDLKEG
                     VKKTAYVMKNTAEDLKDDAQGAMVNLEMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(5601..6365)
                     /locus_tag="JA154_15935"
     CDS             complement(5601..6365)
                     /locus_tag="JA154_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAT7997320.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
BASE COUNT         2639 a          693 c         1066 g         2022 t
ORIGIN      
        1 aaatatagat ttctaaataa gtaatttatc aatattacat atcaaaataa aacgttacaa
       61 attagctgaa aatatattga aagaaaggaa gattttatta tgagttcaaa taaagtggta
      121 gaaagttata aaattacagg tatgacatgt gcagcttgtg caaaggcagt agagagagta
      181 actaagaaga tggatggagt atatgaccaa aatgtaaata tagctactga aaaacttaaa
      241 atagaatatg ataattctaa ggttaatttt gatgatataa agcaggttgt agaaaaagca
      301 ggatatggaa ttataaagga agaaagtaat aaaaaaattg atatgaaaat tgatggaatg
      361 acatgtgcag cttgtgcaaa agctgtagag agagtggtaa aaaaattgga tggagtagaa
      421 agtattagtg tgaatattgc aacagataaa gctaatatag actatgaccc atcaaaagta
      481 aaattatccc aaataaaagc agctattgaa aaagctggat ataaaccaat agaagaggta
      541 agaaataagg ttgatgtgga tgaagataaa ttgagaaaag aaagagaaat gaagtcttta
      601 tttgttaaat ttatagttgc aatagtattt gcagtacctc tgttttatat agcaatggga
      661 cctatgataa taaagcctat agggccatgg cctttgccag aaattataaa tcctatgaca
      721 aatactttta attatgcatt aattcagtta attttagtaa taccagtaat gatagctggt
      781 tataaatttt atataaatgg gtttaaatca cttttttctt taagccctaa tatggattct
      841 ttagttgcaa taggaacatt agccgcattt ttatatagtc tatatacgac actacaaata
      901 gccaatggac aaattcaagg tatgcaccat catcagttgt attatgaaag tgcaggaata
      961 ataatagcac ttatcttact tggtaaatat ttagaatcaa aatcaaaagg aaaaacatca
     1021 gaagcaatta aaaagcttat ggggctacaa cctaaaactg caatagtgtt agttgatggt
     1081 aaagaagttg aaactcctat agaagaagta gagataggag atatactatt agtaaaacca
     1141 ggaactaaaa tacccgtgga tggagttgta atagaaggat atacatcagt tgatgaatca
     1201 atgcttacag gtgaaagtat accagtagaa aagaatgttg gaagtaaggt gacaggagct
     1261 agtattaata aaaatggtgt tattaagttt aaggctgaaa aaattggtgg agatactgct
     1321 cttgcccaaa taataaagtt agttgaagat gcacaaggta caaaagcacc tattgctaaa
     1381 cttgccgata ctgtatctgg atattttgta ccaatagtaa ttgcaatagc tgtagttgca
     1441 tcattattgt ggtttttaat aggaggaaaa gatatagtat ttgtacttac tatatttata
     1501 tcagtacttg taattgcttg tccttgtgca ttaggattag caacaccaac tgctataatg
     1561 gttgggactg gaaagggcgc agaaaatgga atcttgataa aaggtggaga ggcactagaa
     1621 tctgcacata aagtaaatac agtgatattt gataagacag gtactataac agaaggaaag
     1681 ccaaaagtaa cagatatagt attaaataat aatgtaaaag aagaatatct tatcaagatt
     1741 gcttcaagtg cagaaaaagg ttcagaacat cctcttggag aagcaatagt taagtatgga
     1801 gaagagaaga atataaaatt tgaaaaagtt gataatttta aagctatacc aggtgctggt
     1861 atacaagtta ctattaatga tgaaagtata ttacttggaa atagaaagct tatgaatgac
     1921 aataatataa agcttggaga tttagaagaa aaatcaaata tattggcaag ccaaggtaaa
     1981 acacctatgt atatagctgt agatggaaat ttatcaggta taatagcagt tgcagatgta
     2041 gtcaaagaaa gtagtaaaaa agcaatagaa atacttcatg atatgggaat aaaagttgca
     2101 atggtaactg gagataatgc gaaaactgca aatgctatag caaatcaagt cggtatagat
     2161 atggttttgg cagaggtttt accagaagat aagtcaaaag aagttgaaaa actacaaaat
     2221 caaggaaagt ttgttgcaat ggtaggggat ggaataaatg atgccccagc tcttgcaaaa
     2281 gcagatatag ggatagcaat aggaagtgga acggatgtag ccattgaatc tgcggatata
     2341 gtacttatga agagtgattt aattgatgtt ccaacagcaa ttaaacttag tcatgaaact
     2401 ataaaaaata taaaacaaaa cctattttgg gcatttggat ataatacaat tggtatacct
     2461 gtagcagcag gaatattata tgtatttgga ggacctttat taaacccaat gattgcagca
     2521 gcagcaatga gcttaagttc agtttcagtc gtatcaaatg cacttagact taaaaacttc
     2581 aaggcatata agagagatta gattaataaa ttaaataaaa tgtatccaaa agagctatct
     2641 ttaaattaaa aggattaatt tacaaattaa gatagctctt ttttcatgta aaattcacaa
     2701 caataagata aaatataagt aatatacgaa attttaaata aaatagaaag gattgataga
     2761 atgaattcgt caatacttgt aattgaagat gattcaaata tacaagagtt aatatcagaa
     2821 tttttaagtg cagaaggtta tcaagttgat acagcaaatg atgggttgga aggtatacaa
     2881 aagtttaagc aaggaagtta tgatttagta atattagata taatgatgcc aaacttagat
     2941 ggttatggag tttgtaaaat gataagaaag tcatcaagcg ttccaattat atttttaact
     3001 gctttaaatg atgaaggaga ccaacttaaa gggtttgact tagaatgtga tgattatata
     3061 acaaaaccat tttcatttaa tcttcttata aaaagagtag aagcaatttt aaggcgaagt
     3121 aacaagacta taaatgacaa atttatagtt tttgaaaaat taaaattaga tttaaacaca
     3181 tatattgcag agatagatgg agaacctata gaacttacac taaaagagtt taatatatta
     3241 aaagctttga tagaaaagta tccacaagtt ataactaggg aaggtctttt ggatagtata
     3301 tggggttatg actattatgg agatactagg attgttgatg cacatataaa aaatataaga
     3361 aaaaaaatat cattaccata tattaaaact gtaaaaggaa taggatatac acttgagaag
     3421 gatatttgat aagtgggaaa agttaagtat aaagtataag ttattttcaa taacgacatc
     3481 attattaata gcattagctt taataatata tctaatttta tattttttat tgccatcata
     3541 ttatcatgag tataaaattg agtctcttca agaaagtttg aaatctttag tagatagttc
     3601 aattcatttc gatacatata ctttagaaga aagactttat tatatggcta aagaccaaaa
     3661 tttggctatt ttattaaaag ataaccaagg taaaattgta tatggtaaga atgaagtagt
     3721 tattttaaga tatagtaagt acatgattaa tagtcttgag gatgagtata gaacttctat
     3781 acctatatat actaaagatg caaaagatgg tccatatact ttagaactcg ttatgccatt
     3841 acaaccaatt gacgaagcaa acgaagttat aagaaaacta atgccataca tcatatcaat
     3901 agcaatattg atagcgatta ttggagcata tatatattca attgtaataa caaagcctct
     3961 tataaacata atagagagtg agagagaaca ggaatataga aggaaggatt ttgttgctac
     4021 aatatcacat gaactaaaaa ctccaataac cataataagt ggtcaaattg aaggtatgat
     4081 ttatagtgta ggtaagtata aggataggga tacttatctg aaaaaatcgt atgagtgtac
     4141 acaagaatta aaagatttag taaatgaaat gatagaggtt tcaaaatcag aaatacttga
     4201 aaaagatttg aaattggtat caataaatat aagtgaatta ttaaatagac ttgtaaagag
     4261 acaagtattt ttaatagaag aaaaacatat gaaaactatt ttaaaaatag aagaaaattt
     4321 ggaaattaaa gcagaccaag agagaataac taaggcaatc aataatataa taaataatgc
     4381 cataaaatat tctcctgaag aatcagagct tataattaga ctctatgata aaaataaacg
     4441 aataagtaaa aagacttcta atcaaagagt ggttttagaa attgaaaata ctggtgtaac
     4501 tatcgagaaa cgttatttag aagaaatatt taatccattt tatagaatag aaaaatctcg
     4561 ttcaagaaaa actggaggaa gtggacttgg actttatata gtcagccaaa tttttaaaag
     4621 tcatgggttt gattatagca taaaaaacaa agaaaactct gtagtattca cagtagaatt
     4681 taaaaattaa ttacttatta taaagaatat ataactttca ataatagttc attttaaaat
     4741 cacaaaaaat tcataatgaa actgtattat ataaatataa agaagcttaa aaccataaaa
     4801 tacaccaact ataatgatga gatttcctta catcctccat ataagtcatc attatagtaa
     4861 cccaacaatt aagttactat gaatttaaaa aataacaccg agcatataat ctatgcttag
     4921 tgttattttt attttgaaat aaaaaatatt taatttattg aaatgtatag atttagatag
     4981 tttaaactat atttaagact taaaaattat aattgatatt acaaataaat atatgtactt
     5041 tgggaaaaat aaatatagga agtattatat ttacagagga ggtagttttg atggatagat
     5101 taaaaagaga tatgaaaaat atgaaagaag atttaaaaga aggtgttaaa aagacagctt
     5161 atgttatgaa aaatacagct gaagatttga aagatgatgc acaaggtgct atggtaaact
     5221 tagaaatgaa aaaagatgag atgatggaaa gatatgaaca aaagaaattt tctaaagaat
     5281 tagagaagca aatgaagaat gaaatgaaat aagcttttat ttatgaataa caaattattg
     5341 tattcatata aaaagtagcc agtaatatta aatagtttat ttaaggaaat agactttcta
     5401 aaaataactg tattagattt agaaagtcta tttcaaatta gttttataaa tagacataag
     5461 gataaagtag atgttataga actagatttt aaaaaataga tactaaggat aaaataaata
     5521 ttatagaaat atgtttttaa gaatatttat caaataaaaa tttatattta taagaaatcc
     5581 tataaaacat ataattaaac ttataaaaca tttacattaa attctgataa aatatctact
     5641 atatcaggag taggcgtttt ttctgtaatt atagaactaa tatcttctaa atgagcaaac
     5701 ttgaaatttc catcaaattt aaacttttta tcttccataa ctataaatac ttttttactt
     5761 gaatttatta tagccttctt cgtaagacca tcttcaattt caaaagttgt aagtgaagag
     5821 ttgaatacat ctactccaca acttcccata aaagtcttat caaacttata tttcgatata
     5881 aactctatag ttggagtacc gacaaaaccg tctaaactga gatttaataa acctccagtg
     5941 gaaattatat ttaaattatt tggttttgaa gtaactacat ttagtatatc cagcatattt
     6001 gtaacaagtg ttactttttt attactttca gctaacaact tcgcaagcaa gatgttaatt
     6061 gtagagatat ctaaaaatat agtttctcta tcttcaatta aattaaatgc tttttctgct
     6121 attattttct ttgttggaat attaatttct tttctgtctt ttgtatcctg attctctagt
     6181 gtttctctag ctagtacagc tcctccataa attcttctta ttgaagaaat cttttctata
     6241 ttttttaaat cttttcttat acaatcttct gtaacattaa atttagaact gagctctttg
     6301 acagtaactc taccttcttt ttttaggata tttaatatag cttggattct ttcttctgca
     6361 aacattttct acctcttttc gcaatatagt attatagtaa atattataag ataaaataca
//
LOCUS       DACULQ010000186         6404 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.188, whole genome shotgun sequence.
ACCESSION   DACULQ010000186 DACULQ010000000
VERSION     DACULQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.188"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            470..622
                     /locus_tag="JA154_15940"
     CDS             470..622
                     /locus_tag="JA154_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997321.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHSSAFSGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(915..1619)
                     /locus_tag="JA154_15945"
     CDS             complement(915..1619)
                     /locus_tag="JA154_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7997322.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1610..3217)
                     /locus_tag="JA154_15950"
     CDS             complement(1610..3217)
                     /locus_tag="JA154_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT7997323.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3471..4649)
                     /locus_tag="JA154_15955"
     CDS             complement(3471..4649)
                     /locus_tag="JA154_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997324.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4875..5369)
                     /locus_tag="JA154_15960"
     CDS             complement(4875..5369)
                     /locus_tag="JA154_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAT7997325.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5370..6131)
                     /locus_tag="JA154_15965"
     CDS             complement(5370..6131)
                     /locus_tag="JA154_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAT7997326.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT         2154 a         1186 c          695 g         2369 t
ORIGIN      
        1 cctcaattaa gtattccaat catatatgag gataatactt cattcattta aaaatataaa
       61 actattatat attaaaattt cttcaataat aaattattta gattgttact ttgatatatt
      121 atataatatc acaaaggctg atataaaaag acaaaaaggg caattttttt atatcaaaaa
      181 aaatgttaaa ttgatattat ccatatataa attgatatta tccatatagg tgctatattt
      241 aacaattcta cttacttccc ttacacttat tagcatatag ttattaagag tttgttgcaa
      301 aacaacactt agcacacttt aaaaagtgtg gtctaataag taatcaacga agaagacata
      361 ataaagcata aaataggata tacttttcca cgtcatcaaa attgaggatt ttattattaa
      421 tatatgataa aatatgctta ataaattaaa ttttaatggg agaatagtta tgaaaatatt
      481 tagtttcttg ttgtttgtag tgggaatatg tttttgtatt gctggattta tggggcattc
      541 ctcagctttt tctggtgcaa cattgagttt ttttgcaact acaatgatag ctgtaagaca
      601 agtcaaaact aagaaaaaat gaaataggca aatcctaact ttataagtta atattacata
      661 agtaattagt ctaaaattag atactttttc tattacaaaa atacatcatc cactttgtat
      721 tctcttaaaa aaaataaaat agctatgtaa attcaagatt agatagacta actctctatc
      781 catttcattg taaaatatac atagctattt gtattcgatt taaaatactt agattttctt
      841 acataacaaa taatttctat ctgattatta ctcatgttca tttattaacc actcagcaac
      901 taatcattta gtatctattc atccgccata cggtaaccta ccccaacctc agtcaaaata
      961 tattctggct gagcaggatt cttttcaatt ttccttctta tacttgccat aaaaactcta
     1021 agcgactgat tttcactttc atagtaattt ccccaaactt tatctattat aaacttatgg
     1081 gttaagactc ttcctgaata cttagacatc aactcaataa tcttatattc gattggagtt
     1141 aaatgtattt cttcaccatt aattgacaca atatgatttt cataatctat ctttaggttt
     1201 ttaacttcaa aagactttgg agcatcatta tttatattat cctgttgagc tttatttcta
     1261 agagccacac gtactctagc caaaagctct gttacactaa aaggtttagt taaataatcg
     1321 tctgctcctc catctaaagc ttccacctta tctctctcat gctctctcgc tgaaacaaca
     1381 attattttat tagtctttgc acatgcccta actcgtccta ttacctcaat cccatcaata
     1441 tctggtagtc ctaaatccaa aattatcaaa tctgggtcaa gagaatgtat aagtgatata
     1501 gctgttcctc cattatctgt ctctacacac ctataatttt gagtttcaag agatactttt
     1561 ataaatttac aaattggctt atcatcttct acaattagaa ctaatggctt tatttccact
     1621 tgtctttccc ctcctcatca tacataggta tgctaaatct aaatgtagct cctccagaag
     1681 gattattaaa ggcgacaatt tctccaccat gagcttctac tatagattta catatctcca
     1741 atccaagacc aatacctctt ctttcaagat ttctagactt attcttagta taaaacctat
     1801 taaaaatatt cccgatatct tcatttggta ttccttttcc attatctgca acttcaaata
     1861 gtacttgatt tgactctttt tttacagtta gttctatctt agaattttta ggtgtatgcc
     1921 ttatagcatt atcaattaga tttactaaca cttgttctat taataataca tccacatgaa
     1981 cgattattat ctcatctgga atatttgttt ttatgtctct actattggca aactttttga
     2041 ctcttaatat agattcagct attatttcat caacaacctc tggtcgtttt tcaatatcca
     2101 attttccttc atctattctg gtcatactaa gtatattctc aacagaatga gttagccaac
     2161 tcgtatcttc atatatattt ttcaataatt ctactctagt ttctttatct atcacatcat
     2221 cattttcaag tattgttgaa gatgaaccca atatacttgt aagtggagtt cttaaatcat
     2281 gagaaattga cctaagcaag tttcctctta acctctctct ctctgcctca aggtttgctt
     2341 ttttattttt ctcaaataaa ttttctctat caattgccaa cgcaacctgt gttgaaactg
     2401 cttctaatag tatcttttca ttttctgtaa taatatctat cttaaaacac gcaacaccta
     2461 taacaccaag aacatttgat tcttgacctt ttatagggaa ataatatgca ctgctgtttt
     2521 tacaatattt tgtcccaatt ccaactgggt tacctgtcct aaaagcttct tttatagctt
     2581 ccttttctaa tactgaatgg aatatattct cacttccatc attgttacaa atataaatac
     2641 ttggcttacc tagattatta ttctcatctg caatagtcac tattacagtt ctatttaata
     2701 tatttactaa attagaccca caaaattcaa taatttgatt tttatttttt gctttaagca
     2761 acgttttgtt attctcatag agcaatctta tcattttttc tctcttatga gaaagtctca
     2821 cttctctttt tatcctagaa gttaaagtac ttgttataat tgctgccatt agcataatac
     2881 aaaaggtcat aggataatct tttctataag ctaaaaaagt atataatgga tgcgtaaaga
     2941 aaaaattaaa tgatagcact cctattactg aagaaagtat tccaaaaaaa tatccatagg
     3001 ttatacttgt agcaaatagt acacctaata tgtatactag tataatatta gattcattaa
     3061 aacctacctt ttcaaaaact actgatattc cagtagcaat tgtcataacc aatatagttt
     3121 ttatgaagtc cttaataatt atctttttac tgagatagcc atcttcatca taatcatact
     3181 cttttaagtc ttcatgttgt agtcgttcct ttttcattta ctacaattcc tccttattgt
     3241 tactgtttaa attggctcta ttttatataa tacaatttta tcataataaa ttcaatcaat
     3301 acatttaatt aatgtactta aattactaaa aggtgctgtc ttaaaatgaa aataaatctc
     3361 tatttcaaaa cagcacctat atatttaaat tactaattta gtttttacat acttaaatca
     3421 atagttttat tcaatttcat aaattataat acttcaatta taaaacattt ctatccaaca
     3481 agcataaata taaagtgggc acatatacct gcacataaac cacctatagt atgcgaaagt
     3541 atcgctttac cagccaatgg tcttgcatct aaagcatcca tcatacctat gtgtgtactt
     3601 aaatatccac tccaacacat acccattgct gtgaatactg ctatatcatt aggagttata
     3661 gcgcctgatt ttataaactc tggtactaaa gatattgctg ctccaacagc tcctaatgca
     3721 gttacaggga atgctattgc ctctggtgat gtgaatccaa ataatggttc tattatgaag
     3781 ctaatcttat caccaatagc aggtaataat tttatacctt catatgcagc tcctgtgtaa
     3841 acagcttgac ctgtagcagg gtctgtagat ggtccaaaag ttaataacat aactaatgta
     3901 caaactacta aaactcctgg tattatagcc atacccattt caacacctaa cttaccacct
     3961 tcaagtatag catccaatgt tctttggaat acattaccat cccttatttc tctaaactct
     4021 tcaagttttg cacctttatc atttatttgc ttttcagtat catttttagg gtcataatta
     4081 taatactttt tagtaaaagt caacataatt ctaacactaa taatactacc tattattgcc
     4141 ccaacattac ctattatagc tggtagtaca tattctttac cttgagctat cataaaagtt
     4201 gttacaataa gtcccatacc aaaggctgtt cctaaattac ataaagcagg tacttgatac
     4261 ttcttaaaat attgtgtaaa tgttttgtct ttagcaaatg gtataatagc tggattatca
     4321 gataaatatg ttgcaactac tcctgctata ctagcaccag gtaagcccca tataggtctc
     4381 ataagaccct taaatatctt attaactaaa gatattactc caaactctga aagcaatgca
     4441 ctaagtgcac ctgcaagaac tgccatagcc attattaaaa atactgtgtc taataaaagc
     4501 gcatgtgctg tactcattat tactttaaac atcattcctg cacccatgat gcttcctaca
     4561 tatccaaatc ctaccgccaa tagtacaata aatacgaagg tttccatact tactgccttg
     4621 actcttctct tatccgacag cttttccatc ttgaaatctt ccccctttta attttaaatt
     4681 aaggttccta acattggtac aaattttttg gtacaaactg agttctaaaa cttacactcg
     4741 taatgcaagt tgtcagtttt ttgaaataaa aaaaaattac gtttatagaa taagaatatc
     4801 ctctttaatt tggatactct tattctattt tatgttatta tattatcatc cttttgttta
     4861 aaagtcaatt ttttctattt ttgttgttta attttattta tactcttttt atatttttca
     4921 ttttctttta tctttgtaag tactccatat aagttgactt ttctaaatac agtagttata
     4981 aataccaatc ctaatgcaat tgaacttgca cacgataatg tactcacccc atattctaca
     5041 gcttttatat aattattggt caaaacagaa tacatagtat tatatattcc ataacctggc
     5101 actaatatta ttaaacagca aacacttaat gtagttgctg gagtcatata aattcttgca
     5161 cataactcac aatatatcgc aaaacaaact gtagctataa aaaaacataa gttttctgtt
     5221 actcccatat ttaaaagaaa tttatacgaa aaccaagcta tacttccacc gattcctgct
     5281 agtattaagt tttttccttt catattaaat agcactccaa agcttaaggc agtaaaaaaa
     5341 gctgctatta cttctataaa caaatgcact tatatccccc ctaacttgaa gtacaatcca
     5401 agtaccaaga acattcctac tgctatagat acacctacaa aaaatacttc tgctaatttt
     5461 gcaagaccag aaatcaactg tccatctaac aaatctctta atgcattagt caaagcaagc
     5521 ccgggaacta agagcattat tgctcctgct attaaatggt ctaacgtctt tatgaatccg
     5581 agctttaaaa ttaaaattga acaagaagct ataataactg ctccaattgc atttacaaaa
     5641 aagtcattaa gggaagattt acttaaatac ataataacaa acttagtcag aatcccaaca
     5701 gtaaatgcag ctataaattc ttccacattt cctccaaata atatcgaaaa agttgctgct
     5761 gcaatacctg caaaaaatat tgttattaaa tccgagtaat agttatcttc acttatttca
     5821 tctaaaattt tctcacataa ttcaatatct aaattattta tacttattgc tcttgataat
     5881 gagttaactt catgaaccaa gtttaggtct actcttcttg agcttattct tttaattgct
     5941 gaatgtaatt tcccatctac aaatacagat gccattacag ctgttggagt tgcaaataca
     6001 tctacgtggt ctattccaaa agattgacat attctagcta tcgtttcttc aactctatag
     6061 gtttctcctc cactttgcaa catagcttta cctgcatttg aggaaaagtt taaaattcta
     6121 tctatatcca caaaacatat ctcccattct tttatagtat tattcatatt ttaatacaag
     6181 atattattta tttaaactat tttaaataat tcatatccaa catttacatt ttatgaaaaa
     6241 acttgttttt tatgcttgtc aaaatcaaat tttaagcttg tttacaccat aaaatggctg
     6301 aattaatatt tgacaaacac tttcaaccat ttatataacc ttctaaattg tataactttt
     6361 tactacaaat aaaattatat ctcttttaat tctttctctt gagt
//
LOCUS       DACULQ010000187         6365 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.189, whole genome shotgun sequence.
ACCESSION   DACULQ010000187 DACULQ010000000
VERSION     DACULQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.189"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..909
                     /locus_tag="JA154_15970"
     CDS             <1..909
                     /locus_tag="JA154_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HAT7997327.1"
                     /translation="LCCTLSTIFNKKKKLNIYLKNSILNILSIVSIVYFLFVVLWGIN
                     YNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKANETRKLTLQDKNGIV
                     KSNTDYNGVINRAQLGYDNILDILPGVSGSYSKPKYIISSNLMCYTGITGIYFPFTGE
                     ANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHPNIDFNYSGYILALN
                     YTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKINEISNEFNNSYLKAN
                     SVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(1021..1125)
                     /locus_tag="JA154_15975"
     CDS             complement(1021..1125)
                     /locus_tag="JA154_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997328.1"
                     /translation="MWKFYVLKILVKTFIKKDNRNVLTKIVRIEMLYR"
     gene            complement(1383..2207)
                     /locus_tag="JA154_15980"
     CDS             complement(1383..2207)
                     /locus_tag="JA154_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997329.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(2555..3610)
                     /locus_tag="JA154_15985"
     CDS             complement(2555..3610)
                     /locus_tag="JA154_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-en-1-yl diphosphate
                     synthase"
                     /protein_id="HAT7997330.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(3792..4796)
                     /locus_tag="JA154_15990"
     CDS             complement(3792..4796)
                     /locus_tag="JA154_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAT7997331.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(4809..5963)
                     /locus_tag="JA154_15995"
     CDS             complement(4809..5963)
                     /locus_tag="JA154_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAT7997332.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(6127..6330)
                     /locus_tag="JA154_16000"
     CDS             complement(6127..6330)
                     /locus_tag="JA154_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAT7997333.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKSI"
BASE COUNT         2103 a         1148 c          661 g         2453 t
ORIGIN      
        1 ttatgttgta ctttatctac aatttttaat aagaaaaaaa aattaaacat atatttaaaa
       61 aattccattt taaatatatt atccattgta tcaatagtct actttctttt tgtagtgctt
      121 tggggaataa actataatag gattccacta gaaaccacac ttataaataa ctataattta
      181 aaaaacaata cctctatcca atcaaaacaa tatagtgtta aagagcttac taaattatat
      241 aaattccttg taattaaagc aaatgaaact agaaaactca cattacaaga taaaaatggt
      301 attgtaaaat ctaatacaga ttataatggt gtcataaata gagctcaact aggatacgat
      361 aatatcttgg atatattacc tggtgttagt ggtagttatt ctaagcctaa gtatattatt
      421 tcatcaaatc taatgtgcta tacaggaata actggtatat actttccttt tacaggtgag
      481 gcaaatgtaa atatagcaat acctgactta tatattcctt gtacagttga acatgaaatg
      541 gcacatcaaa ggggatttgc tagtgaagat gaggctaatt ttatagcgta tcttacttct
      601 ataaaacacc ccaatattga ttttaactat tctggatata ttttagcttt aaattatact
      661 gcttctgctt taagtaaggt tgattataat gcatatgttg atatttctgc tggcatttca
      721 gattctgtcc gtagagattt aaaaaatgaa agtgaatttt ggcaaaaata tgaaggtaaa
      781 attaatgaaa tttctaatga gtttaacaat tcttacttaa aagctaatag cgttactgaa
      841 ggtactcaga gttatggtaa aatggttgat ttattgctaa cttattatga attatatcca
      901 tataactaaa cactacaatt caaaatttta ttcatataca gctgatatta aatcagctgt
      961 tttctattta aataaattat ttaacttcaa gcaatcttat ataatttgat agtacttata
     1021 ctatctgtat aacatttcaa ttctaactat tttagttaaa acatttctat tatctttttt
     1081 aataaatgtt ttaactaaaa tttttaaaac ataaaacttc cacattattt cactatctat
     1141 ataaaaatct aaaaattcac atataaaatt acataactaa aaaatcataa atttatagaa
     1201 aacatatatt caaaataaaa aagcatgtat caaaacttga cacatgctta taatcttatg
     1261 tatatatatc attttgtcac aataaaaatt tacttgtaac actgatattt ttaaacaaaa
     1321 tagtccttat ttaattaaat taacattaat taaattcgta ctcattttgc tatcattaaa
     1381 cattacttat agtaattttc taatacacta tttgaaactt ccttcccttg aaaacttttc
     1441 aaccaattct catatgctga gtttttccat acactaccat cattaaatgt tacttgtttt
     1501 acacaagata taatgtactt tacaccatgc tctccagttg attgtttcca tttatcaaac
     1561 aatagaaagg catcattttg attcttacct ggtgctattg cattttgctt tgcatattct
     1621 ttctccattt gtttccattc tttcaaccaa ttttctaccc atgccttgcc ttgttgtttt
     1681 tcaaacatac ttattatatc ctgtggtggt gattgttgca tttttctata agtatgtgaa
     1741 tatgattttg gagacacagg agatatgcct gtaacaatac catagtctac accagctggg
     1801 ctaaatccaa cacttcctac ttcgccatct gctgccacat tttgagcatc ccaatatagc
     1861 tcaagtggtt ttccattttt atcatatgct aagcatgtaa tttcataatc cataattgcc
     1921 ttttcactgc tatttgttaa gcttataaca ttgctaggcc aatgatatgg agtatcatca
     1981 tactgtattt tgctattatt tataaataat ggaatattct cattataaac aagaccttta
     2041 ctactttgaa tatctgatac cttctttgtg acagtactta ccgtatttgc taagtataca
     2101 tttgtttttt ctgaattggt cgtactaaga gaaaatgttg ttgtcgttcc agtaatcagc
     2161 aaagcaacaa ctaaaatagg taagctagat atttttttat atctcacaat tcttcacctc
     2221 tttttctatt aacttataat aaaacaattt accaaaacat taaaatctat gttaacataa
     2281 tttaccaact gttctagtaa ctatatccta tcatagttac tatcaagttt caatactatt
     2341 gatggaaatt ttcactatat taaatctttt tttaatttta taaatttaca atatgattta
     2401 actcatacat atatagtcaa attagaatat aagtttctta catttaatga agtttataaa
     2461 ataaaaaatg actagataat taaatactgt aaataaaaac ttattacaaa agtatttaaa
     2521 aatctagtca attaatatat atatgaattg tatactataa tttttcaatc tcttctagca
     2581 actcatcaac taatttgtta ccatcaatca ttctaactat ttcacccttt ttaaataaaa
     2641 gaccttcacc tttaccccca gcaataccta tatctgcctc tctggcttca ccaggtccat
     2701 ttacagcaca gcccataatt gcaacagtga tatttttttc catactacca atcttttctt
     2761 caacctcatt tacaacacta attaagtcta tattacatct accacatgta gggcatgata
     2821 taactttaat cttatcattt aaaagtccca aacttcttaa aatctgttta ccaacaataa
     2881 cctcttcaac agggtcgcct gttaaagata ttctaacagt atctccaatt cctttaagaa
     2941 gaagtgctcc tacccctata gaagacttta ttgttccttt gtgaacactt ccagattctg
     3001 taattccaat atgaagagga taatcaactt tttttgatat taattcatat gcatcaacag
     3061 ttttataaat atcggatgat tttagagata taactatatt atgaaaatct aagtcttcaa
     3121 gaatcttaat atgtcccatt gcactttcaa ctaatgcttc agcagttgca gagccatact
     3181 tttttaaaag ttctttctct aaagaacctc cattaactcc aattctaatt tttaaatttc
     3241 tttccttgca tttctcaact accatcttaa ctctctctat acttccaata ttaccaggat
     3301 ttattcttat tccatccaca ccttgttcta tagcttcaag ggctagctta taatcgaaat
     3361 gtatatctgc aataattggt atatttacac tagactttat ctttgctata tttttagcag
     3421 ctttaatatc aggaacagca actctaacta tatcacaacc agcttcctct aatctcttta
     3481 tttgagctat tgtagcatct gcatctcttg tatcagtatt tgtcatagat tgtatactta
     3541 taggattttc tcctcctatt tttacagttc ctacacttac ttctctcgtc tttcttctct
     3601 cgtaactcat attccacctc tatatattat tttatattga atctctttaa atattaaaat
     3661 attataacct acatgtatat tataaattaa aaagctatct agttaaataa gaataaattt
     3721 catatataaa aattaattta ttcttattta aaactacata gcctattttc ttaaaatctc
     3781 taaaaactaa atcattgaaa taatcttagt atatctttat atgtgataaa aagcataaat
     3841 aacattaaag ctccaaaacc aacaacattt atcatgcctt ctttatttgg gtcgagtttt
     3901 ttaccacctc taacagcttc tagtaacagc ataagtattc tccatccatc aagtgctggt
     3961 attggtaata gatttactat acctaaattt aagcttatga cagctgctaa atacactaca
     4021 tttatgatgc ctgtacgagc agcatctgat accataccaa taacacctac tggtcctgct
     4081 acagcattgc caatccctcc tggtacacgt cctgtaatca tttgacctag aaatgttagc
     4141 atctgttttg tcatatctac agtagtttta catgcattta ctattgaccc caaaaagtct
     4201 ttttctcttt gtggttgaat ccctatttca tactttccat tgttattttt aggtgttaca
     4261 tttacaactt tatcacttcc atctctagtt atactaagtt ttaattctcc accagaagaa
     4321 gtatttatta tatttgcaac ttcatcccaa gtcttaacag aattaccatt aattttattt
     4381 atcttatctc ctacttgaag ccctacagcc tgtgcaggag tatctggcat tatctttcct
     4441 aatttagtcg ttggagaacc tatatacaaa aatactggaa ctaaaagtat tgctgctaat
     4501 attatattga atattggtcc tgctacaata gtactaaatc tttgtaatat agacttgttt
     4561 ccaaaagaat ttggacttat ttgttcttca tcttcacctt ccatgctgac atatccacca
     4621 attggaagtg ctcttataga atactctgta tcttttttta cactatatat cttaggcccc
     4681 atacctatag aaaactcatt tactttgata ccacttcttt ttgcaaatat aaaatgtcct
     4741 aactcatgta ttaatacaat tatactaaat agtattaatg cggctattat agtcataaat
     4801 tcaccttcct atttttttat cagatttgca acataagctc tactccatct atctgtttct
     4861 aagatttctt ctaatgttgg ttcacttatg ctactatgaa cttctaaagt cttctctatg
     4921 taatatggta tgtcatagaa accaatttta tcctctaaaa attcacttac taaaacttca
     4981 tttgctgaat ttaaaacaga ggaataagtt cctcccattt ttaaacactc atatgccaat
     5041 tttaaacatg gaaaagtttc taaatctggt tcttcaaaag ttaaagtact aaattttgaa
     5101 aaattcattc tttcaaagct actttccatt ctatctggat atgtaagagc atactgtatt
     5161 ggtagtctca tatctggaca tcctaattga gcaattattg agctatctgt atactgcacc
     5221 attgaatgaa ttatgctttg tgggtgaact actacatcta tattttcttg ttctactcca
     5281 aataaccatc tagcttctat cacttcaagt cctttattca taagcgttga agaatcaata
     5341 cttatttttc ttcccatact ccaatttgga tgcttcaaag cttcattttt agttatattt
     5401 gcaagttctc cctttttctt tcctctaaat ggaccaccag atgctgtaag tattatcttt
     5461 tctatatttt ttttattttc tccatttaga cattgaaata tagcactatg ttcactatca
     5521 acaggaagaa tatttacatt gtatttttct gcttctttca ttacaagttc accagcagtt
     5581 accaacgttt ctttattcgc taatgctatg tctattccct tctttatagc acaaagagtt
     5641 gggactaatc ctatcatacc tactacagca gttaaaagga catccacttc atcaagtgat
     5701 gatattattt ttaatccttc cattcctgct aaaacttcta tttctatatt tttaggcaac
     5761 atttctttta atttcaatgc tttattttcc tcaaaaactg ttacatattt aggtttaaac
     5821 tctactatct gttctagcaa taattcaata ttactgttag ctgatattgc aactatctca
     5881 aacttatctc tattatctct aactacatcc aaagtttgtt tacctataga gcctgtagaa
     5941 cctaaaattg atatcttttt catataataa tcctttctaa taataaatat accctgtcaa
     6001 aacattataa cactactaaa acaattattt ttactattca tagaaatttc atatattatt
     6061 ccacagatta aaattaatct attatttttt gtattttgtg gaatattata aaatattcta
     6121 gtattattaa atgctctttt ttatttccac aacatcttct attttacagt ctagtgctaa
     6181 acatatcttc tccattgtat ccatagaaac agatttatca tttcctagtc tgcctaaaac
     6241 agaaatactt atacctgata tatttacaaa ttccattttc tttatttttc tgtcaattaa
     6301 caatttccat aatggagcgt atgaaatcat gttatcacct cgcaaaatat attctatcac
     6361 atttt
//
LOCUS       DACULQ010000188         6328 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.190, whole genome shotgun sequence.
ACCESSION   DACULQ010000188 DACULQ010000000
VERSION     DACULQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.190"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..655)
                     /locus_tag="JA154_16005"
     CDS             complement(<1..655)
                     /locus_tag="JA154_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7997334.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     "
     gene            complement(1806..1889)
                     /locus_tag="JA154_16010"
                     /pseudo
     CDS             complement(1806..1889)
                     /locus_tag="JA154_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
     gene            complement(1927..2949)
                     /locus_tag="JA154_16015"
     CDS             complement(1927..2949)
                     /locus_tag="JA154_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7997335.1"
                     /translation="MRLIAPTIIRTSHKIEAPGIDRMEMVEIGGIQQALYIRGQNADN
                     PIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNVGKTYFANDPAAVLQTMSAE
                     RVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQTYPEDYSAYIGIAQTVNMV
                     DNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGPSYLAALMTVRTYQSKYGLS
                     INMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDVLRFLVEEADIRNYGVDYQV
                     PVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDADHLPFLSKKAEFDCVLLEEI
                     APKLAK"
     gene            complement(3090..3746)
                     /locus_tag="JA154_16020"
     CDS             complement(3090..3746)
                     /locus_tag="JA154_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7997336.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            4068..4274
                     /locus_tag="JA154_16025"
     CDS             4068..4274
                     /locus_tag="JA154_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997337.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(4427..4855)
                     /locus_tag="JA154_16030"
     CDS             complement(4427..4855)
                     /locus_tag="JA154_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAT7997338.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(5331..6272)
                     /locus_tag="JA154_16035"
     CDS             complement(5331..6272)
                     /locus_tag="JA154_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAT7997339.1"
                     /translation="MMNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGK
                     NIVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNY
                     NNEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNL
                     KNAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNS
                     NTTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAE
                     LYAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         1953 a         1218 c          760 g         2397 t
ORIGIN      
        1 tatatccaac tatattttct tctaatacta taggtatctc taaactggaa acaccatact
       61 cacaatgata cactttttca aagttatcat tatttatgct tttaacaagt tttttagtac
      121 acggttttat atttttacaa atatcgcaaa atttattttc tttcttagtc tcaaatattt
      181 ctttatactt taagtcatat acagatattc taagcttcaa tatattagat atttgatttg
      241 caagattttc aattatgttt acattaacta actcctgtat attgttttca gaaaatacta
      301 taggtatttg tctcaaactc gaaaatttat gaggaacata agtaattaat attactaaat
      361 caattttacc agtgtttata atttcatgtt ctgaatatgg tgggcaataa actatagatg
      421 tttcctttat attgaccttt ttaccatcaa ttatttgtat tccttctccc tgaatcacat
      481 ataaaagctg ttcttcactt aaatgaaaat gcttttcttg gcagtttccc ggaaaaaatc
      541 ttacaattcc agcactaagt ctattgaatt gtgtttttga tggttcgtgt atccacataa
      601 cctctcccca atcaaatttt tgtacttcat tatttgactc aataaaatcc accataaact
      661 cacctcgtag tttttgtcta aatatttttt cttcccttct tatattaaaa taatatcatc
      721 atcattaact ttcattacta taaaatcatc atactctttt aatactcctg taattttagt
      781 tgcacttagt ttactacaat tcagtttttt aatatattga taaattctat aattactttt
      841 ttattttgta aaatcagtgt tttcccaaaa taattatatc tataaatgaa agtattttta
      901 ctgcttacat caatatatgt tatcataatt ttccttttta ttgcatatat aaataactgt
      961 atataacatt gtccaatact atatagaaat acatctaaga aagaatatta ttaaattgtt
     1021 ccaattaata ttatttttct tatagaaata aaaaactgtt ggattctttt agaaaaatta
     1081 ttttttacat accttttttg cttaaatagt ctatcttctc agctattttg tatatacact
     1141 cttctagttc atgattttat tttaatatct tgcttatatt gctgaaataa ttaaattctt
     1201 ttatctatca tattgtctct tatatcacaa acatattgtt tcttatatca caaaaatgaa
     1261 agcattctta tatcttcaat atataaacct gcttttttta atcttgtaat aaatatagga
     1321 tactctatat tctcttccta gaaataacta ataccactta tttttctatc atatagaaag
     1381 taataaatct ttttcttaat aaaattcaat agtaagtgga ataaaatttt atatgaataa
     1441 gaacattata aaaatacttt atacaatagt caataaaaga tattagcttc aaattgtgtt
     1501 gaagttgtat tatttaaagt ggaaaagcaa ataaaaaaga aataatggta taagtagaaa
     1561 aatatactta aaacaaggcg aagcctctct tgtatctaaa tcaagtagag aattaaatct
     1621 aaaaaagaaa ataaatataa aagatgactc ctgtacatta caagaatcat cttatggttt
     1681 cataatcact ccatggtttt taactgtctg atataaaggt ctcagttcac tcatagcatg
     1741 accacctttt ccgattgctg accccgtcac ttatggacaa aatgtccata agtgatagtc
     1801 ctctcttaaa accctctaaa tagatatatc tcaagcacct cattgaaacc agttgctgga
     1861 aacaaactga agcgttctgg aatagtaaaa gaaattgcca ccatatccac gttactgtcc
     1921 tagttgttac tttgctaact tcggagcgat ttcctctaac aatacgcagt caaattctgc
     1981 ctttttgcta agaaatggca gatggtctgc atctggtata aaaaaaatct gtttgtctgg
     2041 cgccactatt tcatcaaaaa atttcttcgc tggaagatag gggctattat agtcattttc
     2101 tcccatgatg tagtatacag ggacctgata gtcaactcca taatttctta tgtcagcttc
     2161 ttccactaaa aaccgcaata catcaccttg attgtcaatc gcatttggat tgacaatggc
     2221 cgtatttttt aactctctca atgaataata tggggttgcc agtatactga ttatattgcc
     2281 caaatccata tttatagaaa gcccatactt gctttggtat gttcttactg tcataagagc
     2341 tgccaaatag cttggaccat attttgctgg cggatatggt gcaatagctt ccagcgcctt
     2401 gatatctttt tgcttccctg cagctttcac catttcaagt gtcttttcat agcccacgcg
     2461 ctcattgtcc accatattaa cagtctgcgc aatcccaatg tacgcgctgt aatcctccgg
     2521 ataggtttgc acagccatag ttcccagcac tgttccccag ctatgaccta taaggataat
     2581 tttctccttg ttgaactctt gcttcaaata tgctgtcacc tcatggacgt cttgcagaac
     2641 acgctccgcg ctcatggtct gcaaaactgc tgccggatcg ttcgcaaagt atgttttgcc
     2701 tacgtttcgc tgatcccagt tcaccacagt aaaatcattt tcccactcat actggtacag
     2761 atgtaaaaat ggcatcattg aattaccagg acccccatga acccaaagga tgatggggtt
     2821 gtctgcgttt tgtccccgga tatataacgc ctgctgtatg ccgccgatct ccaccatttc
     2881 catgcggtcg attccaggag cttctatttt atggcttgta cgtataatcg tgggggctat
     2941 cagacgcaaa ccggcaaacg ccagaacaaa aacagcaact agtatgcaaa gtgtgattaa
     3001 tatcatttta agtaccttct ttcatgaatt tttttagcca tagatatctc ctctaacatt
     3061 aaaaaaagta tgttgctatt ttagttttgt tactctacct gtcccattgg tttcctttca
     3121 taaaaaatat taattgattc tataatctca tcttccagga tatcaactcc tatttgtctc
     3181 ataagaaact gtaagaattg aaaccgttta cgaagtgtag gaaagtcccc ctgaaataca
     3241 tctgtatcac accaaaagtt aaagagcagc aaaattacct ctgcgcattc atctgggaac
     3301 tctgtgatga tgcttctatc ctctatgcct tctcgtaaaa catttgctaa tattggtgct
     3361 agttttttca aattatcctg tgtaaatgcc agtatgatat gcggactttc tatcgcgctt
     3421 gtaatcatgt tactagattc ttttatcacc tcatccgtta aatgccgcct aactaaactt
     3481 ctaagcttct cttttgctgt aagtccatgc atttcaccga gccattgatt tactgtctct
     3541 attatctgtt caaagcgcct ttccatgacc acattaaaaa tatcttcttt tgaactgaag
     3601 tgatgaaaaa ttccgccctt tgacattccc gaagcatcaa caatattttg catacttgtt
     3661 ttgtcgtatc ctttttcagc aaacaacttt gcagaaatgg taatgatatt ttcaatagct
     3721 tgctttggat tgttctttct agtcattata taactccttc ttattaaaaa ccgactgttg
     3781 gtttttatta tataagctat tcctgcttct gtcaagtaac attgtgttta tggcaaaaca
     3841 ataaggtttg gtggttagac aggtattaat tagactaatg cgactttccc ctagatggaa
     3901 cccttggtaa atttgcaatc tttaccggct ttgattatat ctgtaagcaa ggcaggtatg
     3961 gccacgcctg ccctgtttga ccctcggata ttcattccta tccctcctct ccttagccat
     4021 tacaccaaaa cgccgctcat tgtctccaat ggcggcgttt taaagatatg atttctcatg
     4081 ctgctgaaat atgcaaaaaa tataagatgc cctttattat ttttcctaca atagaaaaga
     4141 aaagtattga agaatgggtt aatattggag caaatatgct ttgttttgca caagatacta
     4201 cctttatcac ttatgttatc aatcaattat tatcttttta taatgagatt gtaccaattc
     4261 aaaaacaaca ttaaattata aatctaattt tatattaaaa gagactaata ttttatctat
     4321 ctctattaag gatattttct tatgtatcat aatattagtc tctcttatta ttatatactg
     4381 ctatcatcat tagttgttat ttatactatt tttataatat acaaatttat tcagctaacg
     4441 ttttgcctaa ttctatacac tcatcttttg cttcttcatt tgggtcttcc attgctgtaa
     4501 ttccttctcc accaataatt gtagcaccag cattttgcat acgttcttcc cagtctctca
     4561 tccattcaca atttccccat ccatatgaac caaataatcc aatctgttta cctgatacca
     4621 tagattctaa ttctgaaaca aatggctcca tatacccctc ttctagttgc tctgctccca
     4681 tagatgggca acctaatgca aatttatctt cttcttttaa atcacttggt ttcagtaagc
     4741 tcacatctaa aacttctggt gtcttacctt ttaacttcac accttcagcc acaaaatttg
     4801 ccattttttc agtatttcct gttccactcc aataaacaat atatatttta ctcattttta
     4861 cctcctgtag tattttttaa ttaatcaata gttagttata gctaactaac atctaaaaaa
     4921 caaaagattt ctttatctga aatctcttta tttttctaaa tatagaatat catagttgat
     4981 attgaaattc aatacttatt gataatattt atcatttata ttttatattc attttataat
     5041 atcaaaacac tgtatcgtat ttctaatctt tcatagaaaa atttctaact tatagtactt
     5101 ttgcatctct tataaatctt ttatatgtat aagtacatag aaaaacacac cttttccaga
     5161 tgtgctatct ataatatcgt atatttattt ttcaattcta actagctaat atgtttataa
     5221 attttatcac ttaatacctt tatacattga tgtaaaatct tacacctaag taatttttat
     5281 tatttgtctc gactctatat aatatagcta ttttatcatc aaatcaaaaa ttatctttta
     5341 ctattattct tgtatttata gagatttaaa tataagtatc catttccttg ttccgttcta
     5401 ggtattccaa gcgctctcgt acattttatt aattgtgcat acagttcagc ttcatctaaa
     5461 tctctttcaa actcctctct tgcccattct ttaattagtg ctaatgagcc tgatacgtat
     5521 ggtgcactca tactagtacc actcataata gcaagtttat tatccatata agttgatata
     5581 atatttcttc ctggagctac caaatctatt gtagtatttg aattactaaa cttttcaacc
     5641 aaatagttct catttattgc acctacttct attacctcag cataactggc tggataacta
     5701 tactcacttg tactagaatc accatctcca ttattacctg ctgcacacac tacagaaata
     5761 ttatttttta ctgcttgcat gacagcattt tttaaattct tatcatcttt gtttccccca
     5821 agagacatag atataatatc aaccttgtta tttacagcaa agttaatagc attaattata
     5881 ctttgatatg ttccggtacc atctttattt aatgcttttg ctattaataa tttacaatct
     5941 ggagctactc ccataacttc attattataa ttagatgcag ctataatacc cgccacatga
     6001 gttccatgac cattaaaatc ctcatatata tttttatttc cattactgtc atcactaaaa
     6061 tttgcaccac caattatttt tccttttaaa agaggatgag atatatcaca acctgtatct
     6121 attataccaa ctacaatatt tttaccagta taaccttcat cccacattcc cctagcattc
     6181 atttgtttta ttccatatgg gaatttattt tttgcacctc ttagattttc atttatttca
     6241 tatggaatta gtttcattct tttattcatc atatatcctc ctaaaaattt tattgcttat
     6301 aaaaatcatg aattttattt tataatag
//
LOCUS       DACULQ010000189         6315 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.191, whole genome shotgun sequence.
ACCESSION   DACULQ010000189 DACULQ010000000
VERSION     DACULQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.191"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            101..1387
                     /locus_tag="JA154_16040"
     CDS             101..1387
                     /locus_tag="JA154_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAT7997340.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1499..1972
                     /locus_tag="JA154_16045"
     CDS             1499..1972
                     /locus_tag="JA154_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1631..1633,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HAT7997341.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2004..3314
                     /locus_tag="JA154_16050"
     CDS             2004..3314
                     /locus_tag="JA154_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3051..3053,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HAT7997342.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3570..5102
                     /locus_tag="JA154_16055"
     CDS             3570..5102
                     /locus_tag="JA154_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HAT7997343.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5113..6258
                     /locus_tag="JA154_16060"
     CDS             5113..6258
                     /locus_tag="JA154_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HAT7997344.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
BASE COUNT         2421 a          764 c         1324 g         1806 t
ORIGIN      
        1 aataatatat ctttaaaaaa ataagataaa ttgtaaaatg atataatttt gagaaaaact
       61 caaatgcata tacacaaatt aaataacagg aggtgcaagt atgcgtcttg aattagggaa
      121 aatctttata aaagatattc aatttggtga tgtaacagaa gtaaaagatg gagtattatt
      181 catcaataag caagagatgc tacaagaaat tggtggagat gagcatatta aatctataga
      241 tatagaatta gctcgtccag gtgaaagcgt aagaataaca cctgtaaaag atgttattga
      301 gccaagagta aaagttgaag gtaatggggg aatcttccct ggtatcatgt ctaaagttga
      361 cactgtagga gaaggaaaaa ctcatgcttt aaaaggtgta gctgttgtaa ctactggaaa
      421 aatagtaggt ttccaagaag gtataataga catgactgga gaaggagcta agtacactcc
      481 attttctaaa ttaaataatg tagtagtaat agctgaacct attgatggtt taaaacaata
      541 cgctcatgaa aaagctgtta gaatgatagg attcaaagca gcaatgtact taggagaagt
      601 agcaagaaat cttactccag atgaagtttc agtatatgaa actaaacctc ttcttgaatc
      661 aataaaagaa tatccagagc ttccaaaagt aggatatgtt tacatgcttc agactcaagg
      721 tttattacat gatacttatg tatatggagt agatgcaaaa caaatagtac caactttatt
      781 atatccaaca gaattaatgg acggagcaat tgtaagtggt aactgtgttt ctgcatgtga
      841 caaaaaccca agttatgttc atataaacaa tggggttgtt gaagacttat atgcaagaca
      901 tggtaaagat ataaactttg taggtgtaat tataactaat gaaaatgttt atctagcaga
      961 taaagaaaga tcttcaaact ggacttctaa attatgtaaa tatttaggat tagatgcagt
     1021 tatagtttca caagaaggtt ttggaaatcc tgatactgac cttataatga actgtaagaa
     1081 gatagaaatg caaggtgtta aaacagttat agttacagat gaatatgctg gacgtgatgg
     1141 aggttctcaa tcacttgcag atgcagatgt aagagctgat gctgttgtaa caggtggaaa
     1201 tgccaaccaa gttgtagttc ttcctaagtt agataaagtt attggacatt tagaagtagt
     1261 agatgtaata gctggtggtt ctgatggaag tttaagagct gatggaacta ttgaagttga
     1321 aatccaagct ataacaggtg caactaatga aacaggattt ggtcatttaa ctgctaaagg
     1381 atattaagaa gtattttatt aaacgattga gtaatgttaa taaaacatat tatagataat
     1441 tataataagt gttatagata tataaaaata tattagaaat ataataagga ggattattat
     1501 gagtttactt agtaataaaa aggttcttat aataggtgac cgtgatggta taccaggacc
     1561 tgcgatagaa gaatgtgtaa aaacagtaga aggagcagag gttgttttct catctacaga
     1621 atgctttgtc tgaacagctg ctggggctat ggacttagaa aatcaaaaca gagttaaaga
     1681 tgctgctgat aaattcggag ctgaaaatgt tgtgatttta ctaggtgctg ctgaagccga
     1741 agctgcaggt cttgcagccg aaacagtaac tgctggagat ccaactttcg ctggaccact
     1801 tgctggagtt gccttaggat taagtgttta ccacgttgtt gaggaaccaa taaaatcatt
     1861 atttgatgaa agtgtatatg aagaccaaat aagtatgatg gaaatggttt tagaagttga
     1921 agaaatagaa gaagaaatgt ctggtataag agaagaattt tgtaaatttt aatgcctaaa
     1981 agcaaaaggg gtgaaactaa gagatgggaa aactaaaagc agtacattat attaatcagt
     2041 tctttgctgg tataggtgga gaagaaaaag ctgatactaa gccacatgtg gcagaaactt
     2101 tacctccaat aagtttacaa cttgataaat tattaggtga agatattgaa atagttggaa
     2161 cagtagtttg tggagatagc tatttcaatg aaaatataga tagtgcaagt gaagaagttt
     2221 tatcaatggt aaaaggtttt gaaccacaac ttttcatagc tggtcctgca tttaatgctg
     2281 gtagatatgg ggttgctgct ggaacaataa ctaaagttgt aaaagatgct ttgaatatac
     2341 cagctttaac aggtatgtat gttgaaaatc caggagcaga tatgttcaaa aaggatgttt
     2401 atgtagtaga aacttctgat tctgcagcag gtatgagaaa ggcacttcct aagatagcta
     2461 aacttgcagt aaaacttgct aatggagaag aaattggaac tcctaaagat gaaggataca
     2521 tagctagagg aataagagtt aactacttcc atgaagacag aggttctaag agagctgttg
     2581 acatgttagt taagaaaata aaaggtgaac catttgaaac tgaataccca atgccaaact
     2641 ttgatagagt tgacccaagt aaagctgtaa aagatttatc aaaatgtaaa atagctttag
     2701 ttacttctgg tggtatagtt ccaaaaggaa atccagatag aatagagtct tcttcagctt
     2761 ctaaatatgg aacttattct atagcaggag ttatggattt aacagaagag acttatgaaa
     2821 ctgcacatgg tggatatgac ccagtgtatg caaatttaga tgcagataga gtattacctg
     2881 ttgatgttct tagagattta gaaaaagaag gtgtaattgg aaaattacat gagactttct
     2941 atacaactgt aggtaatggt acatctgttg caaactctaa aaaatatgct tctgaaattg
     3001 gagcagcatt agtggctgat ggtgtagatg cagtaatatt aacttctaca tgaggtactt
     3061 gtactcgttg cggtgcaacg atggtaaaag aaatagaaaa aactggactt ccagtagttc
     3121 atatgtgtac agtagtacct atttcattaa cagtaggggc taatagaata gttccaacta
     3181 tagcaatacc tcatccactt ggaaatccag cgcttgaccc tacagaagaa aaagccctaa
     3241 gaagaggcct tgtagaaaaa gcattaaatg ctttaactac agaagtagat ggtcaaacag
     3301 tatttgaaaa ataaaaataa cacacacata tattatatag ataaaaatat aaacacacac
     3361 taaaaataaa aaataactat acacaaaaaa ataatataga tttcatgaat ggataaattg
     3421 gagttattca ttcatgaaat cttaaaaaat gccaaaatac ataaacaata atagaatcaa
     3481 atatacaata aaatacaatg attagagctt ttaataagtt taataagatt aaaaaataag
     3541 tgttcataaa atttatggag gtgcacaaaa tgacttatcc agttttaaaa ggggcaggct
     3601 atgtacttat tcatactcca gatatgatag tgcaaaatgg aagtacttgt acagttgaaa
     3661 gagcaacaaa tccagattca gaatttttaa aagaagtaag taatcatata agaagttatg
     3721 aagatgtagt taactacatg ccaaatcaag tttatatagg aaatagaaga ccagaagaat
     3781 taagagattt accaatgcca tggtgtgaac aaaagattga gggaactaga aatggtaagt
     3841 ttggagaaat aatgcctcaa gatgagttca tagctttaat gcaaataagt gatgcatttg
     3901 atttagtaaa attatcacaa gaatttattg atgaagtaaa gccaaaaatc gaaaataact
     3961 accctgaaat tgcaccattt gtaggtaaat taaagggtga tgatatagaa gaaggtaaag
     4021 aattagtagc aacacacata gctgaaggat tataccatga tggaaaattt gttggttatg
     4081 taaaaagagc acatgatgta gatgttaact taaatgccca tacaatgttt gaaaatctag
     4141 tagtaaaagc atctggagtt ttatctgcta ttcaaatgct aagacatagc aaaatagacc
     4201 ctgctgaaat agattatgtt attgagtgtt cagaagaagc ttgtggagat ataaaccaaa
     4261 gaggtggagg aaactttgct aaatcaatag ctgagatagc aggacttcaa aatgcaactg
     4321 gttctgacac aagaggattt tgtgcagcac caactcatgc attaattcaa gcagcagctt
     4381 tagtaaaagc tggcatacat aaaaatgtaa tggtagtagc aggtggagca agtgctaaac
     4441 ttggaatgaa tgctaaagac catgttaaaa aaggtcttcc tgtacttgaa gatgttgttg
     4501 gaggatttgc agtacttgta tctgaaaatg atggtgtaaa tccagttata agaactgatt
     4561 taacaggtaa gcatactgtt ggaactggtt cttcaccaca agcagttatg acagcactta
     4621 taacttcagg tcttgataga gctaatttaa aaataacaga tgttgatgtg tattcagttg
     4681 aaatgcaaaa cccagatata actaagccag caggagcagg agatgttcca gaagcaaact
     4741 acaaaatgat aggggcactt gctgttaaac gtggagactt agaaaagaaa gaattgaaag
     4801 attttgtttc taacaaagga ttaccaggat gggcaccaac tcaaggacat ataccatcag
     4861 gagctcctta tataggattt ttaatagatg atttaactac aggaaataga aatagagcta
     4921 tgatagttgg aaaaggtagt ttattcttag gaagaatgac taacttattt gatggagtat
     4981 cttttatagc tgaaagaaat acaggtgtta ctgaagagac ttcaggaata tctaaggatg
     5041 aaattaagaa gataatagct gaatctatga aaaaactagc attagatatg ttagaagaat
     5101 aggggtgagg atatgtcaaa aaaagtaatc gcagatgtat tcttagaagt agctaatgca
     5161 atagaaagcg gcgaatttgg caaaaaagta aaaattggtg ttactacact tggaagtgaa
     5221 catggtgtag aaaacatggt taatggtgca caattagcaa aatctaattt atttgatatt
     5281 gtgcttattg gaccaaaagt ggaaactgac cttgaagtag ttgaagttaa tgatgaaaaa
     5341 gaaatgcatg caaaaatgga agaattatta gattcaggat atattgatgc ttgtgttact
     5401 atgcattata attttccaat aggtgtatct acagtaggta gagtaataac tccagcaaaa
     5461 ggaaaagaaa tgatacttgc aacaacaaca ggaacaagtg ctactaatag aatagaggca
     5521 atggtaagaa atgccattta tggtattgct acagctaaat caatgggtaa caaatgccca
     5581 aaagttggaa tacttaatgt tgatggagca agacaagttg agaagtgctt aaaagaatta
     5641 aaagataatg gatatgacat ggagtttgcg gattcaatta gagcagatgg tggatgtgta
     5701 atgagaggta atgatttact tgtaggtgca cctgatgtta tggttactga tacattatct
     5761 ggaaatatat ttatgaaagt attttcttca tatactacag gtggagatta tgaagctcaa
     5821 ggctttggat acggacctgg agttggagag gactatgata gaaaggtact tatagtatca
     5881 agagcatcag gttctccggt agtagcaaat gctttaaaat acgcttatga tgttgtaaag
     5941 ggagatataa gtaatgttgc aagaaatgaa tttgcaaagg ttaaaaaagc aaaatttgat
     6001 gacattatat cctctcttac taaaaaagaa gttaaagctg aaaaagtaga agttaaaatg
     6061 ccagataaag aaattgttac tcgtcaaata gctggtgttg atataatgga tttagaagat
     6121 gcagtatctg agttatggaa aaacggaatc tatgctgaaa gtggtatggg ttgtacaggt
     6181 ccaatagttt tagtaaatga agctaaagga gacttagcag ttgaaacttt agttaaagct
     6241 ggatatactg caaaataagt acatttttta tatgtaaata agaacatata gttataattt
     6301 tattgattat aatta
//
LOCUS       DACULQ010000190         6296 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.192, whole genome shotgun sequence.
ACCESSION   DACULQ010000190 DACULQ010000000
VERSION     DACULQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.192"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            197..640
                     /locus_tag="JA154_16065"
     CDS             197..640
                     /locus_tag="JA154_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7997345.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            671..2488
                     /locus_tag="JA154_16070"
     CDS             671..2488
                     /locus_tag="JA154_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HAT7997346.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            2666..5368
                     /locus_tag="JA154_16075"
     CDS             2666..5368
                     /locus_tag="JA154_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAT7997347.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            5402..6106
                     /locus_tag="JA154_16080"
     CDS             5402..6106
                     /locus_tag="JA154_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7997348.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDEWL"
BASE COUNT         2483 a          707 c         1151 g         1955 t
ORIGIN      
        1 tttattaaaa atatcctgta taaaattaaa tatcaataag ataacaaaat ttcaattgaa
       61 gttacaatat tgtaattgac aataaatact taatactata atatttaata cattaaacat
      121 tatagtgtta agtatttatt acagtatgca cttcagccta aatttgaaat aaagtatttt
      181 cgtagggagc gtttgtatgg atgagatgag ttttaagaaa gagctactag agttgactag
      241 agatataaat atgaaattta caactttatt aagtgatttt tatcagccac ttggtattac
      301 agcagtgcaa gctttgattt tatctgagtt atgtgaacat ggagagaaaa aaataagcga
      361 tttagggaag aacttaaaca tgacaaatag taatgtatca gtaatctgtc aaaggcttga
      421 aaaaaatggc tttttaaatc gcataagaga tatagaagac caacgaattg taaaagtaaa
      481 agttacaaat aaatcattgg atatacaaga acatatttca agtagtattt ttgatagtta
      541 ttttgaaaat atgaccagtg aaaaattgca agatatggaa gatataatag aaggtttaga
      601 gaaattaaat aaacttttaa cttacattga ttgcaaataa cataaaaata atttaaagga
      661 gaataaaatt atggatgaac ataaacccca aaaaaggtca gcactctatt attatacagt
      721 tataataata gtagtaatgt ttttaaatgc tttaatattt ccaagtttaa tggataaaaa
      781 aatggttgag attgactatg gaaagtttct cacaatgttg gatacaggaa aggttaaatc
      841 tgttgagata gaaaataata gaatagcaat aaatccatct agtagcaagg ataagaatat
      901 atacataact ggtagaatgg atgacccaga attggtagat agactaaaaa atgcaaaagt
      961 agaatttact aaggttattc ctaaggaaaa ttcacctctt ttaagtattt tacttacatg
     1021 gataatacct ataggtattc ttatgctatt tggtaactta atgatgaagt ctatgcaaaa
     1081 gcgaatgggt ggtaatgcta tgcaatttgg aaaaagcaac gccaaaatgt atgtatctgc
     1141 tcaaagtgga aaaagattta atgatgtagc tggtcaagat gaggcaaaag aagctttaac
     1201 tgaaatagta gatttcttac acaatccaga aaagtataaa aagataggag cacaaatgcc
     1261 taaaggagca cttttagtag gacctccagg tacaggaaaa acactccttg caaaagctgt
     1321 agcaggtgaa gcaaatgtac catttttctc aatatctggt tctgagtttg ttgagatgtt
     1381 tgtaggaatg ggagcttcta ggattcgtga tttatttaaa caagcaaaag aaaaagctcc
     1441 atgtatagtg tttatagatg agattgatac tattggtaaa aagcgtgata atggaagtgg
     1501 attaggtgga aatgatgaac gtgaacaaac tttaaatcag ttgttaagtg agatggatgg
     1561 ttttgatggt ggaattggag tagtaatact tgctgctaca aatagacctg acagtcttga
     1621 caaagcatta cttcgtccag gaagatttga tagacgtata ccagttgaac ttccagacct
     1681 tggaggacgt gaagctatat taaaggtaca tgctcaaaat gtaaatgtaa gctcagatat
     1741 agattataat gcaattgcaa ggtctacatc aggagcatca ggtgcagaac ttgcaaatat
     1801 tgtaaacgaa gcagcacttt tagcagttaa gtctggcaga gatatagtag aacaacagga
     1861 ttttgatgaa tctgttgaaa ctgtaattgc tgggtatcaa cgtaaaggag ctgtaatttc
     1921 agataaggaa aagaaaatta tagcatatca tgaaataggt catgcacttg ttgctgctat
     1981 gcaaaaacat tctgcacctg tccataagat tactataata cctagaactt caggagcatt
     2041 gggatatact atgcaagttg ctgaagatga gagtgtactt atgtctaaag aagaagcaat
     2101 tgataaaata actactttta caggtggtcg tgctgctgaa gaggttatct ttaacacttg
     2161 tacatcagga gcatcaaatg atatagaaca agctactaaa attgcaagag ctatggttac
     2221 tcgccttggt atgagtgaag agtttgatat ggtcgctctt gaaactgtaa gcaatcaata
     2281 tttaggagga gatacttctc ttgcttgttc ttccgacact gctgctaaaa tagatgaaga
     2341 ggtatttaaa atagtaaagg cttgtcacaa aaatgcaatt gaaatcttag aaaataatat
     2401 tgataaattg catgaacttg ctaattattt attagaaaaa gaaactatta caggtgatga
     2461 gtttatggaa atacttaact taaactaaaa tgattataca caagataaaa atgcctattg
     2521 ttaggcattt ttattttaca actaaatttt ttaaattcat atttaaatct ttacataatc
     2581 ttaatcatta aaaatacatc ttaactacaa ttttatatcg gaattgatat tataatagta
     2641 atgaatggtg taaggaggtt ttttaatgga tagacctaat cctgatattc tattagaaaa
     2701 aattaaaaat gaagaagaaa aactaagtag aggtcaatta aaaatattct ttggttatgc
     2761 agctggagta ggaaaaacct actctatgtt ggaatctgca caaaatttaa aaaaagtggg
     2821 tgtagatgta gtagttggat atattgaacc acacactaga ccagaaacat tggctttact
     2881 tgatggcttg gaaattcttc ctgtaaagga gatagaatat aaatctataa aactaaaaga
     2941 atttgattta gatgctgctt tagcaagaaa accagaagtt atattagtag atgaatttgc
     3001 ccattctaat gttgctgggt taaggcatac aaaaagatgg caagatatag aagaattgct
     3061 gttagcaggc ataaatgtct acactactgt aaatgtacaa catttagaaa gtctaaatga
     3121 cattgttgag ataattacaa acgtatcagt taaagagact ataccagata agtttttgga
     3181 tacaaataca cagttagaac ttatagatgt tgaacctgat gtacttcttg aaaggttcaa
     3241 tgaaggtaag atatataaga aagaacaagc tactagagca aaaaataatt tttttataaa
     3301 agataacctg gttgcactta gagaaatcgc acttagaaaa actgcagaga gagttaataa
     3361 agaagttcaa atgaccagac tatcaaaggg tgatgtaact gtaataccta catcagatac
     3421 acttcttgca tgtataagtc catctccatc ttcagcaaaa gttattagaa ctgcatccag
     3481 aatatcagac agtacatttg caaaatggat agctttatat gttgaaactc caaacacagc
     3541 aagacttcca tttgatgaac aaaaacaact acaaaataac ctaaaattag ctaagaaatt
     3601 aggtggagaa ataattgttt tacatggtga aaatataata gagcaaatac ttagaattgc
     3661 aaagctgaga aatgtaacaa aaattgtaat aggtagaaat cattcaagta ataaaagatt
     3721 tagtaaaaga tttaaaaaag atatagttga caaattaatt gatgaagttg attatataga
     3781 tatacatata attccatata aatttgcctc agatgttaaa tacaaaccta aaaaggataa
     3841 atcatcaata aaatctaaat ttaaaattag caaagttgat tttataaaac ttatatttat
     3901 tactttgatg ataagtattt tagcatatgc agtacaaagc atgggattta taagagaaaa
     3961 tatattgtta atatatatgt tgggtgtagt tttagtttct atgtggacta aagggtatag
     4021 tacaggtata ataagttctg tatttaatat agttctttta aattattttt tcacagcacc
     4081 attatataca ttatctatag cagattcaaa ctatatagta acactggttg tattctcaat
     4141 agtaggtata ataactagca ctcttacgtc taagatacag catgaagcag aaactgctgc
     4201 taaaagagaa gaaaatacaa aaatgatata tcaaataatt aagggatttt taagattatc
     4261 aaataaagat gatatagtaa acaaaggtat agaacttttg tctcttagct taagtagaga
     4321 tgtaatttgt tatttaaatg tagataagca taataaatca aagctttaca aaaaaaatac
     4381 taataataag gataaaaatg atttggatag tgaagatgaa aaagctgttg caagttgggt
     4441 tttatccaat tcaacagtag caggaaatga cacggataca cttcctggtt caaagggata
     4501 ctatatacca attgtaggaa tgaatttaac tttaggagta gtgggaatat catgtattga
     4561 ttcaaaattg gatacagaag atataagctt aattgaaaca ataattgctc aaatggcaat
     4621 agcgcttgat agagaaatct tgtcagaagc taaagaaaat accaatcttg agattgaaag
     4681 ggaaagatta agaagtaatc ttttaagagc agtttcgcat gaccttagaa ctccactagc
     4741 aggaatatct ggtgcagtaa gtacaattat aaaaaacaag ggtactatag gtcaagatat
     4801 aatagatgaa ttacttagtg gtgtatatga agatactcag tggttaataa gacttgttga
     4861 aaatttactt agtatgacca agatagatga agggaaattg gaagttaaaa aacattctga
     4921 actggttgag gaaattatat cagaagcgtt acaaaaaata aaaaaaagaa ttgaaaatgc
     4981 acttatagat attaatatac cagaacagat attatttgtt cctatggatg caaaattaat
     5041 agaacaagtt ttaattaatt taatagataa ttctttgaaa tattcaaagg aagattgtaa
     5101 gatagagata aatgtatatg agaaagatga ttatgtttgg tttgaagtat ctgataatgg
     5161 tcctggtata tcaaaggaac ttaaaaagca tatctttgac agatttttta ctggagaaga
     5221 aggagctaaa gattccagaa agggtgttgg acttggatta tcaatttgta aatctataat
     5281 tcaagctcat aaaggtgaaa ttatggtaga aaacaataaa gataagggtt caacttttaa
     5341 gtttagtttg cctaaggaaa atgaatagtt ttaaatttta aaattagttt tggaggaaga
     5401 aatgagtaaa aaacttatat tgttagtaga ggatgataag acaataagaa aatttatatc
     5461 tactgcactt ttaacacagg attatgatgt caaggaggct ataactggaa aagaaggtat
     5521 atctatagct gtctcatatt ctccagatgt tgttctttta gatttaggct tagaagatat
     5581 ggatggaata gaggtaataa aagctatcag acaattttca aatataccaa taattgtagt
     5641 ttcagctaga gagcaagata gagacaaggt agaagtattt gatgcagggg cagatgatta
     5701 cttaacaaaa ccattctcca tagtagagtt attagcaaga gtaagagtag catttagaca
     5761 ttcacaagta gaagctcaac aaaaagatga tgttaaatct acgtttgaag tagataaatt
     5821 attgattgat tttgataaaa gaaaagttat tgttgatgat gttgaagtcc atttgacacc
     5881 tattgaatat aatattcttt cattacttgc aaagtatcat ggaaaagtgc ttactcataa
     5941 ttttataata aaagaaatat ggggtagtgt tatagggaat gagactaagt ctttaagggt
     6001 ctttatggca acgttaagac gaaaaataga aaaacaacct gcaaacccaa gatatataat
     6061 tacagaagtt ggtgtaggat acagattaaa tgatgaatgg ttgtaatata ttaattatta
     6121 gattatgtgc ttattaatat atattaaaat tatatagttt agttaaaata agaatgaagt
     6181 gtatgtgctt gttgtcagtt tagcacatgc attttatttt ttagcttttt tagtatcaat
     6241 aattttttag tatcaataat atgaaatatg ttttaaaata agtatatagt gtaatc
//
LOCUS       DACULQ010000191         6225 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.193, whole genome shotgun sequence.
ACCESSION   DACULQ010000191 DACULQ010000000
VERSION     DACULQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.193"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            160..1104
                     /locus_tag="JA154_16085"
     CDS             160..1104
                     /locus_tag="JA154_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HAT7997349.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1126..1752
                     /locus_tag="JA154_16090"
     CDS             1126..1752
                     /locus_tag="JA154_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAT7997350.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1786..2835
                     /locus_tag="JA154_16095"
     CDS             1786..2835
                     /locus_tag="JA154_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAT7997351.1"
                     /translation="MREKESIRELKGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2838..3416
                     /gene="hisB"
                     /locus_tag="JA154_16100"
     CDS             2838..3416
                     /gene="hisB"
                     /locus_tag="JA154_16100"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088051.1"
                     /note="catalyzes the dehydration of
                     D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate to
                     3-(imidazol-4-yl)-2-oxopropyl phosphate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HAT7997352.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3418..4023
                     /gene="hisH"
                     /locus_tag="JA154_16105"
     CDS             3418..4023
                     /gene="hisH"
                     /locus_tag="JA154_16105"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088052.1"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAT7997353.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4020..4742
                     /locus_tag="JA154_16110"
     CDS             4020..4742
                     /locus_tag="JA154_16110"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088053.1"
                     /note="catalyzes the formation of
                     5-(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino-l-
                     (5-hosphoribosyl)imidazole-4-carboxamide from
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAT7997354.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4724..5482
                     /locus_tag="JA154_16115"
     CDS             4724..5482
                     /locus_tag="JA154_16115"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088054.1"
                     /note="catalyzes the conversion of
                     5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-
                     1-(5-phosphoribosyl)imidazole-4-carboxamideand glutamine
                     to imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamideribonucleotide and glutamate;
                     the HisF subunit acts as a cyclase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAT7997355.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5510..6178
                     /locus_tag="JA154_16120"
     CDS             5510..6178
                     /locus_tag="JA154_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP pyrophosphatase"
                     /protein_id="HAT7997356.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2538 a          531 c         1237 g         1919 t
ORIGIN      
        1 atagacacaa gccctgtttt tagtgtaatt ggtcttgtat ctatgatggg aagtaatttt
       61 aagagaaaat tatagccata taggtataaa gcctatgtgg cttttttatt gttttaaatt
      121 tgcaaatata agagtaaatt taatgaagag ggtgagaatg tgaaattttt gaggattgaa
      181 gatgaggtta tttattctaa aaaaagatat aaattaaaaa gggaaataga taaacttttt
      241 ttggatgaag aatattttca aatagaacca caattatttg aagaatatga tgaatttaca
      301 actataaata ataaaattcc agaagaatct atggtaaagg ttgtaaatgg aaaagtaatg
      361 gtacttagag ctgatataac tacaagtata ataaaaagcc ttgtaccaag atgggaagat
      421 ggtttaaagc ttaagctttt ttataattca tcaatatata aaaataaaaa tacagttgga
      481 ataaaagaga taagacaaat aggttgtgag tatctaggag aagcctctgt agaagcagat
      541 agagaagtag tgaaattagc tcttaagata cttgaaaaat acaataataa ttttattcta
      601 gaggttggga gtagcaagta tattcatggt ttgttagaag aattaaattt aaataagaat
      661 tgtgaaaatc aaattaaaaa tttactatat acgaagaaca ctcatgaact aaaagtatat
      721 attgaagaat taaaaattaa gcgtgaagta aaagagttat tatcaaatat tttaagttta
      781 caagggaatt tatataatgt tattgaaaaa tcaaacaagt tctattgtaa taataagatg
      841 aagcaagctc ttgaagaatt aaagcaagta aataacttga ttgaggaatg taatttttta
      901 gataaggcta gatttgattt atcaatgatt actatgcttg actattatga aggaattatg
      961 tttagaggat actacccaaa ctcatataaa gaaattctta gtggtggaag atatgattct
     1021 ttaactaaag agtatggaaa agaaattcca gccattggat ttacactaag tgttgatgag
     1081 ttaatgaagt atgtatataa gtaaagttta gaaggaggga ttgctttgga ttatttgact
     1141 atagcactag caaaaggacg aattgaaggg gaatcattta aaaaatttaa aaaaatgggt
     1201 ctaggagaca gtatagacac agatacgaga aaactaattt ttaaagatga agaaaataaa
     1261 attatatata tacatgtaaa gccatcagat gtagttacat atgttgaaaa aggagtcgct
     1321 gacctcggta tagcaggtaa agatactatt ttagagaatg agacagatgt atatgaaata
     1381 tatgatttag gttttggaaa atgtaaattt gctgtagcag gattaaaggg agatagcata
     1441 tatagagaag atgaatattt aaaagtggca acaaagtatc ccaatatagc aaaaaagtat
     1501 tttaaagaga aaggtcaaaa aattgaaata ataaaattaa atggttctgt agaactagct
     1561 ccaatagtag ggttgtcaga tgtaattgta gatatagttg agactggcaa tacacttaaa
     1621 gcaaatggtc ttgaaatatt agaagacata tgtaatataa gtgctagaat tatatcaaat
     1681 agagctagct atagatttaa atatgaacaa attcagaata taattcgttt atttgaagaa
     1741 cttgataatt aaaatataat tgagctttat aaggagggat ttaagttgag agaaaaagaa
     1801 agcataagag agttaaaggg atatgaacca aatcacgtaa actgtaaagt aaaacttgat
     1861 gcaaatgaag gaagtaagag gttgtttaaa taccttatta aagaaatttc agatagtgat
     1921 atagatttaa atttatatcc tgaagattct tatagtgatt tgaaagaatc tataatagat
     1981 tacataaata tttctggagt aaataagaaa aatcttctag taggaaacgg ttcaagtgaa
     2041 ataattgatt taataataca tacttttgta gataaagatg aagtaatatt gagtttctct
     2101 ccaagtttta gtatgtattc tatatatagt cagataaatg ggtctaaatt tataggtgtt
     2161 gaaagtgatg aaaatttagt tataaatata gataatgtga ttgaaaaagt aaaggagaat
     2221 aatcctaaaa tagtaattgt atgtaatcct aataatccaa ctggaacaat attaaagagg
     2281 gaagagataa taaagctttt agattcaact aatagtctag tggttttaga tgaagcatat
     2341 atggattttg gagaagaaag catgttaagt gatgttttta agtatgataa tctaatagtt
     2401 cttagaactt tatcaaaggc atttggattg gctggaataa gaacaggata tatgttatct
     2461 aatagtagtt taataaattc tgttgagaaa gtaagacctc catataattt aaattcatta
     2521 tcagacttta ttgctacaag agcacttaga aataaagatg tggtaaaggc ttatataaag
     2581 gaagtaaaag aggaaagaga agttttatat aaagaaatga ttggtatggg aattaaagca
     2641 tataaatcac aagcaaattt tatattattt tattctgaaa tcgaaaattt atcacaaaaa
     2701 cttattgata gaggtgtttt aataagaaaa tttggtggaa agttagaaaa ttattacaga
     2761 gttacaattg gtgataaaga agaaaattct atgtttgttg gagctataag agatattttg
     2821 aaaaaggaga aataggtatg agaatttgga aggtagaaag aaatactctt gaaacacaga
     2881 ttttagtaga attaaatatt gatggttctg gtaaagctga aatagatact ggaataggat
     2941 ttttagacca tatgcttacc ttaatgtcat ttcatggcaa gttcgattta aaagtgattt
     3001 gtaagggtga tacatatgta gatgaccacc atagtgtaga agatataggt atagctatag
     3061 gtgaagcatt taaaaatgct ctaggtgaca aaaaaggaat acgaagatat tctaatatct
     3121 atatacctat ggatgaatct ctatcaatgg tagcaataga tataagcaat agaccttatc
     3181 ttgtatttaa tgctaaattt gatacccaaa tgattggtag tatgagtact caatgcttta
     3241 aagaattttt tagagctttt gtaaatgaat cgagagtaac attacatatc aatctcttat
     3301 atggggaaaa tgaccatcat aagatagaaa gtatttttaa agcatttgca agagctttaa
     3361 aagagggtag tgaaatagtt tctaatgaga tagcatcttc aaagggggtt ttgtagaatg
     3421 aatataatag tagactatgg tcttggaaat atagattctg tgtctagggg ttttagaaaa
     3481 gctggaatag aaactaaaat ttctagtgat atagatgaaa taaaacaggc agattcactt
     3541 atacttccag gtgttggagc ttttagagat tctataagtg ccttagataa actaggattg
     3601 ataccaataa taaaggagca tgtttctaag ggtaaattta tgataggtat atgtctagga
     3661 atgcaattac tttatgaaaa aagttatgag tatggagaat atgaaggatt aggacttata
     3721 aaaggaagta ttgataaact ggatataagt ttaaaagtgc cacatatggg atggaataat
     3781 ctaaaattta ataaggcaaa tgatgatata cttaaatata taaatgaaga tgattatgta
     3841 tacttcgttc actcatatta tgcaaattca tcaaatgaag aactaattgc attttcagaa
     3901 tatgaaaaga aaatccctgc tattgttaga aaaggcaatg tatatggtat acaatttcat
     3961 cctgaaaaaa gtggagaagt agggttaaat atattaagag catatgggga gatgataaaa
     4021 tgataatatt tccagcaata gatataaaag ataataagtg tgtaagactt acacaaggag
     4081 aatttgacaa agtaaatgta tattatgata atccattaga agtagcatat aaatggaaaa
     4141 atgaaggagc agaatatata catatagtag atttaaatgg tgctagaagt gaatttggtg
     4201 taaatacaaa aataattgag gacatagcaa acaatataga tatacctatt caagttggcg
     4261 gtggagttag ggataaggaa aaagtcaaaa gtttaataaa tgctggtgtt actagagtaa
     4321 tattaggtag tatagcgatt gaaaatttga atttagttga agaattggtc aatgaatata
     4381 aagagaaaat tgtagtttct atagatgcta aagatggaaa agtagcagtt aggggatggg
     4441 aagttgtaag caatgtagac tcattaacat tatgcaaaca acttgaaaag ataggtgtac
     4501 aaactattgt ttatacagat atatcaaaag atgggatgtt gcaaggtcca aattttgaca
     4561 tatatgaaag aatagcaaag gaaacatcac taaatgttat tgcttctgga ggcgtaactt
     4621 ctatagaaga cgtaaaaaga ctaaaagcta tgaacttata tggtgcaata ataggtaaag
     4681 cgctttatga taaaaagata gactttaagg aggcacaaca attatgctta ctaggagaat
     4741 aataccttgt ctagatgtga gaaatggaag agtcgtaaag ggaaaaaaat ttaaagatat
     4801 agtagatgtt gatagtccag aagtacttgg aaaattttat agtgattgtg gtgcagatga
     4861 acttgtattc tatgatataa ctgcatcaaa tgaagagaga aagacatctt tagaatttgt
     4921 gacaaaagtt gcagaaaata taaatatacc attttgtgtt ggtggaggag taaataaatt
     4981 agaagatttc actgatattt taagaaaagg tgctgataaa gtttctataa attcatcagc
     5041 agtcaaaaat cctgaattaa taagggaagc ttcattaaaa tttggagcac aatgtgttgt
     5101 tttatcgata gatgcaaaga aaaatgaaga gggttcatgg agcgtatacg taaaaggtgg
     5161 aagagaaaaa actaatctgg atgcaattga atgggctgtt aaaggtgtag aacttggagc
     5221 aggagaaatc gttgtaaata gtatggatga agatggaatg aaaaatggat atgatataga
     5281 attattatca aagataactt ctttagtaaa tgtacctgtc atagcatctg gaggcgctgg
     5341 aaaaaaggaa gatttttatg aggcggttaa taaatccaat gtagatggaa ttttagcagc
     5401 atctgttttt cattttggcg agataaaaat aaacgattta aagaaatatt taaaagacat
     5461 gggtgtagaa gtaagattat aagttaaata ctaaaaggag attagaaata tggataataa
     5521 gtgtaacaat gtatacagtg atgaagtaga gaaatttata agaagtataa agtttgatga
     5581 taaagggtta gtccctgttg ttgttcaaga ggtagttagc aaagatgtac tgatgttagc
     5641 atatatgaat aaagatgcta taaagaaaac cttaaaagat aaagtagctt gctattttag
     5701 tagaagtaga caagaacttt gggttaaagg tgagacatct ggtaatactc aaaaagtagt
     5761 taagatgtct tatgattgtg atgtagatac tatactactt tttgttgaac aaactggtgt
     5821 agcttgtcat acaggcaatt attcttgttt ttacagagac ttgtttgatg atagttcaaa
     5881 aatggagctt gaagtacaaa caaatatatt gaaagaatta tatgatttaa tcaatgaaag
     5941 aaaaaataat ccagttgaag gttcttatac taattatctt tttgaaaaag gaatagataa
     6001 gattcttaaa aaagtaggtg aagagagtag tgaggtaatt atagcatcaa aaaataccga
     6061 taaaagtgaa cttatttacg aaattagcga tttagtatat catacattgg ttttaatgat
     6121 agaaaaaggt gttgaaattg atgaaataaa aaaagaatta cttaaaagaa gaaaatagaa
     6181 taagatagat taatgggtaa taagaaaata taccactttt aataa
//
LOCUS       DACULQ010000192         6224 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.194, whole genome shotgun sequence.
ACCESSION   DACULQ010000192 DACULQ010000000
VERSION     DACULQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.194"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..542
                     /locus_tag="JA154_16125"
     CDS             <1..542
                     /locus_tag="JA154_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997357.1"
                     /translation="KNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDNLNKEKNANPKE
                     IGLLSKTKLDYMVKNNKLNLIVTDISNSITNLVYDINSNEIKLEDYEILDLKVDYNEA
                     SSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQVFDMKLKKNIYE
                     AELINGDISLQEKLFFVAN"
     gene            908..2089
                     /locus_tag="JA154_16130"
     CDS             908..2089
                     /locus_tag="JA154_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAT7997358.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(2231..3148)
                     /locus_tag="JA154_16135"
     CDS             complement(2231..3148)
                     /locus_tag="JA154_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997359.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            3401..4195
                     /locus_tag="JA154_16140"
     CDS             3401..4195
                     /locus_tag="JA154_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997360.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            4196..>6224
                     /locus_tag="JA154_16145"
     CDS             4196..>6224
                     /locus_tag="JA154_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT7997361.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPALCEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGL"
BASE COUNT         2396 a          683 c         1020 g         2125 t
ORIGIN      
        1 gtaaaaatgg atatattgaa ggacatgata gttactctaa ctttattata tatgatatag
       61 aagataacac ttttaatata agtaataaat ttatggataa tctaaacaaa gaaaagaatg
      121 caaatccaaa ggagattggc ttattaagta aaactaagct agattatatg gtaaaaaaca
      181 ataaattgaa tcttatagtt acggatatta gtaattccat aactaatctt gtttatgata
      241 ttaattctaa tgagattaaa ttagaagatt atgagatact tgatttaaaa gtagattata
      301 atgaagcatc atctggagtt aggaaagtta agctactgga tgataagata tattctattt
      361 caggtattaa gaacagtata tcaggagaat taaaaggatt taaagggatt gttgatgtag
      421 tcggaactaa gccagtaaaa tttcaagtat ttgatatgaa acttaaaaag aatatttatg
      481 aagctgagct aataaatgga gatataagtt tacaagaaaa gttatttttt gttgcaaatt
      541 aagtgttaga attaatttta gaactttaaa cttttatatg tatcacagat aagtataagt
      601 aagggagtgt ctcaaaataa actttttagc tcatgagaca ctttttttat atgaaatcaa
      661 atatattttc attgtttaga tttattttga taatgaaaaa aagattatct ttattttaag
      721 aaagacattt atttataatt atatacaaaa atatttttta atgtttttgt gtataattat
      781 aacaatttaa tttatattaa tgcacttaaa tttgaaatag aatgcttata gtggtaaaat
      841 taattattag aatattagaa ataataaata aataatacat tgtacttaaa agggggagaa
      901 atttaagatg aacaaaaaat atattaaagc agggaattta attgatggaa ccggaaatag
      961 tattatgcaa aataagggta taattattga tggagatact atagttgaaa ttgaagatat
     1021 aagagatgat ttaaatggat atgatgtgta cgattactta gataaaactg ttatgccagg
     1081 cattatgaat tgtcatgttc acttgacaat ggagccagtt ggaaatccaa agatatatta
     1141 tgataatgta tcagatgtag agcttgtagt aaatacgata aaacaacttg atgcttattt
     1201 ggattcagga gtaacatata ttagaagctt aggttgtccc aaatatgtgg atgtacaact
     1261 taaaaatctt atagacaaag ggtctataga tggtcctggt atagttactt caggtccagt
     1321 tatatgtatg acaggagggc atggatatta ttttggtatt gaaagtgatg gagtggatga
     1381 atgtagaaaa tcagccagaa cagttttaaa aaatggagta gattgtgtaa agataatggc
     1441 aactggaggt gtaacaactg atggagttga gccagggtca ccacaattaa ctttggagga
     1501 aatgaaggct gctgtagatg aagctataaa agcaggtaaa ataactgcca cacatgcaca
     1561 aggaagaact ggtataaaaa atgcagtgct tgcaggaatt acttctatag aacatggtgt
     1621 ttatttagat gatgaaataa ttgatttgat gcttgaaaga ggaacttact tagttccaac
     1681 tgtggctgca ccatatttta ttctaaatgc aggaaaagaa agtggaataa ctgaagctac
     1741 tcttagaaag tgtgagattg ttgccattcc acataaagag tcattcctaa aagcatataa
     1801 aaaaggaatt aaaattgcag ttggttctga tgcaggaact tcttataatg aacatggaaa
     1861 aacatattat gaaatgaaac taatgtcaga ttatggtatg gataatatgg atataatagt
     1921 tgctgctaca aaaacagcat ctgaactttt aagaattgat aaaaattatg gaactctaga
     1981 aaaaggaaag aaagcagatg ttatagttgt aaatggaaat ccagtagaga atatagacgt
     2041 acttgcagat gtgttagcag tatttaaact tggtaaaaaa gttagataaa tagtataaaa
     2101 ataaaaaaga gtgtctaaag aaatttttaa ataattatag acactctttt ttattagtaa
     2161 taattaaatc ttttatactt tagaaaatag attctatttt atatttagat ataactcttt
     2221 aaaatcattc ttatgcaata taatctatag aatctacaca tacaaagtat attcttgaat
     2281 cattagaatt tccaagtact ataacatcat ttttcttacc tagaacttca acaccagtta
     2341 gaaccaaact accagctact acagtaactt gattatttat atttgacata taaagattat
     2401 ctaggatacc tttattacaa cagcaatcat cacaatcgca gttgcaatta cattctttgt
     2461 aatgtttttt atcaagatag taacctaaag atgctatgaa atcttcataa tctgcaggtg
     2521 taatatcaaa tgcaacagct ttaagattac acaatgatgc taaatctacg tttgctaatg
     2581 gagtagatgc agcaagtata aaatcaatta tagcagcaag tattgcatct aatatagccc
     2641 caaggtctat cgtaggagga ataactgcat caataagtgc aattaattca gctactcctg
     2701 gtatagttcc tatagtatta attaatccct caagagttaa agggactgga ataggataag
     2761 atactctacc agctatatct attaaatcac agttacaagc agaaaatctt tcaaaagtac
     2821 catcaagtcc agataaatta tcttttggag gagctgaaag atctatacct accaaattag
     2881 cacctactat aaagaaatct gagataaaag caaattgatt aaagtttaca aaaggtctta
     2941 gagcatcata tcttagaagt tctaatgctt ctctcatact ttttttacaa caacattgtt
     3001 ttattttagg ttctttatat ttatttggat aattatagta tctatcagct acttgataat
     3061 tttcatagta tctttcattt gtatttggat aatcttcctc atattcttgt gtaaatctaa
     3121 agtcctctct acatttatta ttttccacta aatacactcc ttttatattt taatttaaat
     3181 gtatagataa ttaagcttat ctatatagta atattatatt tataatgtaa aaaaatgtta
     3241 caaaagatat tataaacgaa taaaaaataa taatttgcca gataaacttg taaattttta
     3301 tgaataatcc attctaaaac aaaaaactgt gtataaaaaa ggtttatatg ttatatttat
     3361 ataaatataa catatgaacg agaaaggagt ggtttgataa atgattgagg tagataaatt
     3421 gtgtaagtct tttacaaggg ttgtaaaaga tgataagaat aaaagttcaa taagaaagct
     3481 aaagaaagta aaaactaaaa aagaagaatt ttttgctgta aataatgttt catttaaagt
     3541 tatggaaggt gaaattgttg gaatattagg tccaaatggt gcaggaaaga ctacattact
     3601 tagaatgtta ggtggcatat taacaccaac atcaggaagt ataaatattt ctggatatga
     3661 ttattcaata gatagaaatt cagcaaaaaa agaaataggt tatctctctg gaaatacaaa
     3721 actatatgga aggctttctc cgagagaact tctaacaact tttgcaagtc tatatgagat
     3781 gtcaaaagaa gatatagaag aatcaattga aaatattgta aaaataatgg atatgagtga
     3841 atttatagat aatagaattg aaaatctatc aactggtcag actcaaagaa catctatagc
     3901 tcgttgttta atacattctc caaaagtata tatttttgat gagccaacac taggtctgga
     3961 tgttttaagt agtgcatcta ttatagattt tatgaagaat gaaaaactaa ggggaaaaac
     4021 tgttctttac tcaactcatt atatggaaga agcagaaact ttatgtgaca agatttttat
     4081 gattcacaat gggaaaataa ttgcatcagg aacaccacag agtttaaaag aggaatctgg
     4141 agtaaataat ttgagagatg tattcataaa acttgccaga aaggaggaca tttaaatgag
     4201 aagtaagatt gtaaaataca tatttaaaaa agaaatgtta gacatattga gagataaaaa
     4261 gactttattt atgatgattg ttgtgccact tcttttatat cctttactta tgttattaat
     4321 gattcaagta atgaatatgt ctgcaagtag tatgaatagt aaggacatta atatagcttt
     4381 taataataaa cctaataagg ttttagtatc catgattgaa gatgataatt tagaaaaaga
     4441 tactaaaaat acttcaaatg ataaaggcaa aataagagtg ttagatgtag ataactataa
     4501 aaagtcatta gaagaaaata aaattgatgc ttatataaag atggaagaaa aaaaatcatc
     4561 tataaattat caaatatata taaattcttc tcaagaaaat tcttctaatg cactaaaaag
     4621 aattgaaact gttttggata aatataaaag atttaccata gaaaaaactt tatctgaaaa
     4681 aggattagat gtacaagcta ctttagaacc aataacatat agcactgtag atgttgcaaa
     4741 gacggaagaa gtggcaggat actttttagg tcagatactt ccatttatat taataatagg
     4801 agttttacta ggttcaatat atccagcaat agatgttatg gctggagaaa aggaaagagg
     4861 tacattagaa acattattta cactgccaat atcaaactta gaactggtta tgggaaaata
     4921 tatggcagtt tctcttagtg caatagtaac agcaatttta aacattttat ctatgggact
     4981 gactatggca tttattttag ttagtggagg tattagtagc caatttggct ttggaaactt
     5041 taattatgga gacttagtct taccaattat aactacttta atttgcatat gtcttttttc
     5101 aatggtagtt tcagcaataa gtatgtgtgt atgttcatta gcaaaaagtt ttaaagatgc
     5161 tcaaaactac ataacacctc ttatgttaat tattatgata ccatcatatg tttctatgat
     5221 accaaatata gtattagata gagtgacagc tgtaatacct gtagtaaata tatctttact
     5281 tataaaaagt gtattatctt ttaaaagcga tttaaattta atagccttag ttttagcatc
     5341 aaatgtagcc tttgttatat tggcaatcat actactgtct aagatgttca attctgagga
     5401 aatacttttt ggtaatagta agagtttctc tttcttagag aaaagaagta atataaaaaa
     5461 agggtctata ccaactgtaa gtgatggtgt tattttatat gcagtaggat tagttctatt
     5521 gatttatgtt ggttctttag ttcaaattaa atttggtatg gcaggtatta taatgacaca
     5581 attcatgata atttctttac cgttattatt tgcatactat attaaagctg attttaaaga
     5641 ggtatttagc ttgaaagttc ccaaattaaa gcatgtgttt gggagcattg ttctttggat
     5701 tggtggatac atacttatta tgattataac acagctaatt ttattcttat ttcctcaaaa
     5761 tatggaagtt gctgaaagtt taaataaggc tctttttatc aaagatagtg ttttactgaa
     5821 tttactagca atagctttat taccagcttt atgtgaagaa atgtttttta ggggatttat
     5881 attttcttca tttagtaaat caaaagataa aaataagtct atcaagctag ctataatatg
     5941 cagtggggta ctatttggta ttatgcatat ggattttatt agaataattc caacatcaat
     6001 attaggtatt atatttgcat atagtgtgta taaatctggt tcaatatttg tatcaatgtt
     6061 attgcatttt ttaaataata gtgtagcagt tgtgttaaat cattatacaa ctggaaatat
     6121 aaccaactta tataaatttg ttgaagttga tttttcgaac ttaaatgtac ttaaatttgc
     6181 cttaattctt ctaataagtc ttatattaat tatgttagga ttaa
//
LOCUS       DACULQ010000193         6165 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.195, whole genome shotgun sequence.
ACCESSION   DACULQ010000193 DACULQ010000000
VERSION     DACULQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.195"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..504
                     /locus_tag="JA154_16150"
     CDS             <1..504
                     /locus_tag="JA154_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997362.1"
                     /translation="DYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQ
                     SKILQFMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIE
                     IGTLFFIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKNYT
                     RKVWFFD"
     gene            623..2014
                     /locus_tag="JA154_16155"
     CDS             623..2014
                     /locus_tag="JA154_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT7997363.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            2121..3161
                     /locus_tag="JA154_16160"
     CDS             2121..3161
                     /locus_tag="JA154_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HAT7997364.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            3650..4303
                     /locus_tag="JA154_16165"
     CDS             3650..4303
                     /locus_tag="JA154_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAT7997365.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVNASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            4379..4588
                     /locus_tag="JA154_16170"
     CDS             4379..4588
                     /locus_tag="JA154_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997366.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(4728..5294)
                     /locus_tag="JA154_16175"
     CDS             complement(4728..5294)
                     /locus_tag="JA154_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997367.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            5455..6108
                     /locus_tag="JA154_16180"
     CDS             5455..6108
                     /locus_tag="JA154_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAT7997368.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
BASE COUNT         2394 a          623 c         1003 g         2145 t
ORIGIN      
        1 gattataaaa actggcaaag cagtgcaaag ataactgaaa atttaaaaga aaaacatttt
       61 aatttggata gagaatataa caggtcatat tttttaacat taccagatat gtatttagta
      121 gagttacaac aaagtaaaat tcttcaattt atgggtgttt ttataggagt gatattattt
      181 atagcagttt ttagtttttt atacttcaga ttatatacag acgagataga agataatgta
      241 aagtacaaaa aactatcaaa aataggtcta tcttttaaaa atatgaataa gattgttagt
      301 atagagatag gtacattgtt ttttataccg tattttatag caattataaa tagcataatt
      361 tctttactgt ttttaaatca agtattaaat aatagtttta agttagaaaa tttgggtata
      421 ttactactga tatcatctat atattcactt tatttttact tattaaaaaa aaattatact
      481 agaaaagttt ggttttttga ttaaagtggt atataataga tatataaatt aatatttaaa
      541 aagtagttaa tattagggga gggcatttgc acctcccttg taatatgtgg aaaattgaat
      601 attagaagaa ggaggagact tgatggaaaa tcaacaatta ttaggaacag aacgaattag
      661 taaattacta ctaaaatatt ctataccagc tataataggt atgttggtaa atagtttata
      721 taatgtagta gatagaattt ttattggaaa tataccaggg gttggaccac ttgcaataac
      781 aggtcttggt gtaacaatgc caataatgac aataatactt gcatttggga tgttgatagg
      841 tataggaact actactacca tatcaataaa acttgggcaa ggtaaagtag aagaggcaag
      901 aaaacttata ggaaatgcaa tgacgttatc tgtaataaca ggaattatca taatgatact
      961 tggaatatta tttgcaaata aaatacttac attgtttgga gcaagtgaaa atactttaat
     1021 atatgcaaaa tcatatatca atattatatt attaggtaca gtagtaaatc ttctttcctt
     1081 ttctttaaac cactcaataa gagctgatgg tagtcctaaa atttcagcag gaataatgat
     1141 agtaggatgt ttaactaata tagttttaga ctggatatta atatttggat tcaatttggg
     1201 aatacaagga gctgcaattg ctacagtaac ttctcaggct cttacagcta ttttaactat
     1261 agggtattat ataagtggaa aatcaaactt acgattcagt aaatctaatc taaaattaga
     1321 taaaaagcta ataaaagcag tgtttgcaat aggtatgtcg ccatttgcta tgcaacttgc
     1381 agcaagttta gttcaagtaa tatcaaatat tgcacttaaa actcatggtg gagatttagc
     1441 tataggggct atggctacta tttcttctat tgcaatggta tttttgatgc ctatctttgg
     1501 aataaatcaa ggagcgcaac ctattatagg tttcaattat ggagctgaaa aatatgatag
     1561 ggttaaaaaa gcatatttag gttcattagt agtagctact ataattttat gtatgggtat
     1621 ggtagtagtt atgcttttcc cagaagctat tataggtata tttaataaag accctgaact
     1681 tatgaatata tctgttaatg gattaagaat atacttactt atgttgccaa ttgtaggctt
     1741 atcagttaca ggaacaaact ttatacaatc tataggaaaa gcaaaaatgg ctatgttatt
     1801 gagcctttta agacaagtaa ttttattgat accagcagta ttaattcttc ctacattctt
     1861 aggtttacag ggtgtatgga cagctcaacc agtgtctgat tttatagcta cagtaattac
     1921 aggtatagtt gtatttagag aattaaaaag atatactcct aaaactgaaa aattaaatga
     1981 aaatgaaaga ctaaacgaga taactactga gtaaaaatat aaattcagat aattgagcaa
     2041 gaaattgcaa aaaatacgat tatatgataa aataaaaccg ctgtcaagga taagagaaat
     2101 tctaattatg gagtgatttt atggaattta caaagaaaag caaattaaaa aatatgtatg
     2161 taaacatgaa tgtagctaga atgttgtcta agataaatga gtataagggt agacaactgc
     2221 tttacaaaaa acaaccaaaa gaaattttag aaaacttaga aaaaaaatct ttagtagatt
     2281 gttctgaatc tacatacatt ggaaatcaaa gagatagtag taattttaat ctagaaaagt
     2341 tgataagtaa tgaagttaca cctagaagta gagaggaatt gagtattgtt gaatacagag
     2401 atgtggtaaa aactattaac agtgcttatg agagtattcc catcagttca caaacaattc
     2461 ttgagcttca tggatactta tataaatttt catctactag aggaggaagt tataagtcag
     2521 ataatgattt tattgaacac aatttaaaac ctagcgaatt tataagtact gattcaagtg
     2581 tcaataaagt tgaaaaagct gtagaagaaa tatgtgaagc ttataataca ttgatagagg
     2641 aagatgaaat tgatatatta atattaattt ctgcatttgt gttagatttc atattgatac
     2701 atccattcaa agaaggtaat ataaaaatgg cgagagtact tatcctattg ttactaaata
     2761 aaaatgggta tgaagttgga agatatataa gtttaggaaa gatatttgat gatagttcat
     2821 atgaatatta tagtaattta aattatttga aagcctcaat aggtagtgaa aaagcagata
     2881 tgaatgcatg gattgagtat tttttagaga cgatattaac tgcatatgag aagctggatg
     2941 atagtttaaa tatatctgat aagaaaagac aaacaaaaac tagtagaata gagaaaataa
     3001 taaattctac ccttggatat tttacaaaag aagatataag agatttatgt cctgacattc
     3061 cagaacctac tataaataga gtttttaata atctaagaaa acaagataag atagaagttg
     3121 ttgcaagagg aagaagtgca aaatggaaga aaaaatatta aaaaatgttt agataactaa
     3181 aatggtatag tgttttacac tataccattt ttaatgtcat tagcatgagc aataatttaa
     3241 caacttaagc aatatcaatt ttatttattg attatttttg aactgaaaaa tggcgttata
     3301 tggtatacct cagtggtaat taattttgga tgagaagata taatattaca gaatatatta
     3361 ggagtatatg tagaaaaaat ataataaaag tgtgaggctt agtaattaac aaattatgtg
     3421 gcttcacact tttttataaa ataaagatac ttagcagaat aatattttag ttgatatgtt
     3481 tataagatgg tacttgtttg ggtaaaagta taaatagtaa aaaagtagaa gtaatgtgta
     3541 taaaaacata agttacattc tgtaataata gttattttat aattcaacaa aagaatagaa
     3601 tgggtatatt tgtgaaataa taatataaaa taatcttagg agtgagctta tgaagaaaaa
     3661 attattatat ataaatgtaa attcaaaacc tgaagactta tcttcaagta aaactgtagc
     3721 aagaaaattt ataaataaat ttatggaaaa aaataaagat tttgaggttg aagagataga
     3781 cctttataaa gaacatatac caagacttga gtatcagtac tttgaaaaaa gaaatagcat
     3841 agttagtgaa gaaaatgcta aaaatttaga cgataaggat agaaaagagc ttacaaagat
     3901 tagaaatctt tgtgaccaat ttgtgaatgc aagtgtttat gtaatagcag cacctatgtg
     3961 gagtttatct tttccagcac cacttaaaga gtatatagat tgtataattc aagatggaaa
     4021 aactatatct tttgaaggca atgataaacc acaaggaatt ttaaatgata ttgatagaag
     4081 tatggtatat atacaatctt ctggtggaca cataccatgg gtgttgaagc cagttatgaa
     4141 taaagggtta aattatgtgg aaagtattat gaaatttata ggaattaaaa aatttgatga
     4201 attgttagta gatggaactg gaactagtga agaagaaaga caagctgcaa tagaaaaagc
     4261 atctgaaaaa atagatggta taatagatgg gatgaaattt taattataat attttattaa
     4321 aaatataaag gaaaaacctc ttttaatgta gaaacttata ttgaaggagg cgagaatcat
     4381 gcgagaagaa aaaagtaatg aaaagtatga ttgttattgg tgtaatcaag agaataattt
     4441 ctgtgtagaa ataaaagata atatagtcat gatagatgat ggcactggta cgttaaaaca
     4501 agccgttttc atagggtata aacaaatcca aattaattta aactgttcac attgtcaaaa
     4561 cttaaataga ataaaattaa atttgtagaa ttttaaaatg gaaattattg aaggaattaa
     4621 aaatagaaag tgttaatatt tagactgaaa attatatttt atacaataaa agtttattta
     4681 aataacattg tattttaata tttattttca gtcttacttt attaaaatta ttttttggtt
     4741 atgtctggaa aatatctgtc tactaatttt tctgaaatat tttttaattg gtcagccatt
     4801 gaagtcagac ttaataaggc tatcaataaa tcatctttat tttctggatt tatactaata
     4861 ttttcaaaat tagactctat aaagtccggt atattctttc tttgattttc ttttattaac
     4921 atgaattttt cgtaacatga aactaaatct ttttcactga aattatcctt ttcatacaca
     4981 ccatgtaaga ttcttttaat ttcttgtatt gtaagtgtat ttttcataga ataaataatt
     5041 atcatctgta aaatttgttc tttagtatac ttttttccct taactggttt gattaatccc
     5101 tctttgctgt aattatttat catggtttta gtaagaattt tatcactttc aaatctttta
     5161 ttagcagaaa gcttattgtc aattagagaa ataatttgat ccatatataa gtcaatagat
     5221 ggaatatcgt ttgaagttat atctgcatta tttagtgttt ctagtattat agtattaagt
     5281 tcattattca tcatatgtta cctccataat atatataata cactaaatta gaaagtaaaa
     5341 caatattaag gcttgtaaaa aaaacataaa gtggtataat taaatatata atatagtaat
     5401 caaaactata tttatgaatc tgatttttaa aatacataat tgtaggagga cgaaatgtat
     5461 caattttttt taaagggtag agacccaata agcagtttaa ctcattttat aggtgcttgt
     5521 ttatctcttt tagcaactat aattttagtt tttcaatctg ttacattaca ggaaacttct
     5581 ctcttgatga tagtatctgt atctgtattt ggactttcac taattgcttt atatagtgca
     5641 agttcgtatt atcatttttt aaaggggaca ccagaacaag aattgttttt tagaaaagta
     5701 gaccatgcaa tgatttacgt attgattgct ggctcttata caccaatatg tctgaatttt
     5761 atggaaaaaa aagaaggcat aatatttgtt acagcaatat ggatagttgc atttattggc
     5821 ataataataa aaatattttg gatggatgca cctagatggt tatctaccag tatatattta
     5881 cttatggggt gggcaattgt ttttgatata aatgcattca attcaattcc taaggattgc
     5941 cttagactat taattatgga aggtgtatct tactctatag gtgcgattat ttatataatt
     6001 aaaaagccta atataagtcc tgagtttgga tttcatgaaa tatttcatat ttttattatg
     6061 ataggttctt tatttcactt tttagcagtt ttattgtatg ttttatagtt ttaaaagatt
     6121 tgattaattg atactaaaat tctacaatga ataaaatgga actgc
//
LOCUS       DACULQ010000194         6079 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.196, whole genome shotgun sequence.
ACCESSION   DACULQ010000194 DACULQ010000000
VERSION     DACULQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.196"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            44..928
                     /locus_tag="JA154_16185"
     CDS             44..928
                     /locus_tag="JA154_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT7997369.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1027..2202
                     /locus_tag="JA154_16190"
     CDS             1027..2202
                     /locus_tag="JA154_16190"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087318.1"
                     /note="Catalyzes the formylation of AICAR with
                     10-formyl-tetrahydrofolate to yield FAICAR and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HAT7997370.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2330..3184
                     /locus_tag="JA154_16195"
     CDS             2330..3184
                     /locus_tag="JA154_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997371.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            3228..4463
                     /locus_tag="JA154_16200"
     CDS             3228..4463
                     /locus_tag="JA154_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAT7997372.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSQSKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLI"
     gene            4564..5505
                     /locus_tag="JA154_16205"
     CDS             4564..5505
                     /locus_tag="JA154_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HAT7997373.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(5724..6077)
                     /locus_tag="JA154_16210"
     CDS             complement(5724..6077)
                     /locus_tag="JA154_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAT7997374.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
BASE COUNT         2345 a          607 c         1118 g         2009 t
ORIGIN      
        1 aaattaaata atattaatca acttattgaa tggaggaata aaaatgaaat ttgtgacttt
       61 ttgtagtagc aatgaagaaa aaataggtgt atttaattca gaaacaaata gtatatatga
      121 aattaatagt ttaggattaa gtaaattata tactgatatg aatgatttta ttgaaaacgt
      181 atctacagga gatttagaaa aaataaaaaa caattcattt gaaaacgcaa agtgttataa
      241 gttagaagaa gttaaattat gttccccaat tgtaagacct aaaaaagata ttatatgttt
      301 aggtctaaac tataaagacc atgtcaatga aatcccagat ggagttataa aaaacgtagt
      361 tatgccagat tatccaattt atttttctaa gagagcagac aaaataattg gagtggatga
      421 taagataagc ttacatggag atttggtaga gaagttggac tatgaatcag aacttgcagt
      481 aatcataggt aaagaaggta taaatatatc aaaagaagat gcttatgaat atatatttgg
      541 atatacaata gtaaatgata taagtgaaag agcacttcaa gataaacatg ttcaatggtt
      601 tagaggaaaa agcttagaca cacatacatc tatgggacct tgtatagtac ataaagaaga
      661 atttgaacat cctttaaaat tagatataag tagtgtagta aatggagaag taagacaaga
      721 ttctaataca gagtacttta tatttgatat acctactata ataaatgatt tatcaagagg
      781 aatgactcta aagccaggag atataatttc aactggaact ccagcagggg ttgctatggg
      841 gatgaatcct caagtatatt taaagcatgg cgatatagtt gaatgtaaag tcgaaggaat
      901 aggagtttta aaaaatatag ttgattaatg gataaatatg ccatataatt ttattagtac
      961 gaataattta attaatatag gattaatcaa acttatgatt aatttattat agagagggat
     1021 gagtatatgg caagagaact agaattaaag tatggctgta atccaaatca aaaaccatca
     1081 aagatttata tgaagaatgg agaacttcct atagaggtat taaatggaaa acctggatac
     1141 ataaattttt tagatgcatt taatagttgg caacttgtga aggaattaaa agaagctaca
     1201 ggtcttccag cagcaacttc atttaaacat gtaagtccag caggagcagc agtaggggta
     1261 cctttatcag atactttaaa gcaaatatat tttgttgatg atttagagct ttctccatta
     1321 gcttgtgctt atgcaatggc aagaggagca gatagaatgt cttcttatgg cgattttata
     1381 gcactttcag atgtatgtga taaggagact gcaactatta tagcaagaga ggtttctgat
     1441 ggtataatag ctccaggata tacagatgaa gcccttgaaa tattaaaagg aaagcgtaaa
     1501 ggcaactata atattgtaaa aatagatgaa aattatactc ctgaacctat tgaaacaaag
     1561 gatgtttatg gaattacttt tgaacaaggt agaaatgaaa tattaataaa tgaagaccta
     1621 ttaaaagata ttccaactga taataaaatt tttacagata gtgcaaaacg tgatttaatt
     1681 atagctctta ttacattaaa atatacacaa tcaaattctg tttgttatgc taaagatgga
     1741 caagtaatag gggtaggtgc aggtcaacaa tcacgtattc attgtactag acttgcaggt
     1801 aacaaagctg atacttggta tcttcgtcaa catcctaaag tattaaactt aaaatttaaa
     1861 aaggatatag gtcgtccaga tagagataac actattgatg tatatctttc tgatgattat
     1921 atggatgtgc ttgctgatgg catatggcaa aacttttttg aagaaaaacc agagcctcta
     1981 actggagaag gaaaaagagc atggcttaaa actttaacag gagtagcact tggttcagat
     2041 gcatttttcc catttggaga taatatagaa cgtgcaaaaa gaagtggggt aagttttata
     2101 gcacaaccag gtggttctat tcgtgatgat aatgttatat taacttgtaa taagtataac
     2161 atagtcatgg catttactaa gaatagatta ttccatcatt aaaatactga aagtataaag
     2221 ataatataga ttaagtaaaa atctacaaca agttttgttg tagatttttt tatttgctgt
     2281 ataattttaa atagaacttt atttttagat attcaatttg gaggttgata tggctttaaa
     2341 tataagaaac tatagcctat tatgtaatca aggaacagct aaatttaatg aagatgtagt
     2401 cggtataaat ccatttgggg catgggtttt agatggtgct acaggactta atggaaaaaa
     2461 tttagtgtca actgaaagtg acgcaaggtg gtatgttcag tggtggaata ggtatttata
     2521 taaaaatata aataaagaag aatcattaaa gagtattatt aatactggaa taatagaagt
     2581 aaaaaaagag tatgaactaa gattaaatgg gtttaaggtt gaaaaattag atttaccatc
     2641 tagttctata gttgttgtaa aatttcatga aaataaagta gagtattttt tattaggaga
     2701 ttgtgctttg tttattaaaa atggagattc caaagttata aaagatagga gtatttgtaa
     2761 atttgataat actgtatttg atgaaatgag taaactttct aacttggaag agatggcatt
     2821 tgatgagatt agaactagtg ttatggatac aatagtaagc aatagattga agaaaaatac
     2881 aaaagagggc tattggatat tggattttga tgaggaagct gtggaaaatt ctatatatgg
     2941 atacattgaa ataaaaaaag attttcaatt gatgctaacg agtgatggtt ttacaagtgc
     3001 atgtgataga tataatctaa taaaagaaga agaattgata cgtatagcag aaaaatttgg
     3061 tataggatat atacataata aagtaaggga ttttgaagat aatgactata aagcggttaa
     3121 agtgccaaga tttaaagtaa aagatgattc atcttgttta tatttagata tatataatga
     3181 ttagactgta taaaactatt taattttaga aaggaggcaa gtgaaaagtg aaaattcttc
     3241 atataataac acaaaaacca aatagtacag gtagtggtat atatttgtct ggtatgataa
     3301 aaggattcga aaaaatcggt cataagcaag ctgtaattgc aggtatagat gtaaatgatg
     3361 atgtaaattg ttttccaagt gaagtaagtt tttatccagt aaagtataat tgtggggagt
     3421 taaattttcc tgttgtaggt atgagtgatt ctatgccata tgaaagtaca agatataaag
     3481 atttaaatat agacatgata aatagattga aatatcaatt taaagtaaat atagataagg
     3541 ctatgaatga ttttaaacca gaacttataa tatgtcatca tttgtacctt ttaactgcat
     3601 ttgttaggga aatggtaaaa gatataaagg ttatgtctat ttgtcatgga acttgtttaa
     3661 gacagttaaa taccattgat ttagagaaag aatacataat agcaaatata aggaaattag
     3721 atttaatatt tgctcttcat gaaaatcaga aatatgatat tataaagact tttggagtaa
     3781 gtcaaagtaa agtagttgta attggtagtg gttataatga tgatatattt tataataaaa
     3841 attataaaat aaaggatgat aaaataaaaa tagtatttgc aggtaagata tgtaaatcaa
     3901 agggactgat tccatttata aaagctatat ctaaattaaa atatagtaaa gatttaatag
     3961 aagtcaattt tgcaggtact ggaagcgata tcgaatcata taatgagata gttaaactgg
     4021 caagtaagtc tccatttaaa atgaatttcc taggaaagtt agaacagaga gatttagctg
     4081 agctattcaa caggtcacaa atatttgtgt taccatcatt ttatgaggga ttaccagttg
     4141 tagttttaga agctttatct tgtggcacag atgtaatcac tactgatatt cttggtgtta
     4201 aagagtggat tggtagtgag attaataatt ctggaaagat agaatatgtt tctctacctt
     4261 ttatggagaa agaaggtatt ccaaaagatg aagagttata tgattttgaa aataatttgt
     4321 ataatgcaat agattctaag atacaatctt tattgaataa ctcaaataag aaaacatctg
     4381 tagatatgtc aaaaaaaacg tgggatggct tagcatatag aataaatgaa gttattttaa
     4441 tggatgaatt gtgtttaatc taaaattatt taaattataa aaaagtgtaa ctatttttac
     4501 actattttta aatgatttta tattatacta atactaaata tatttgtttt agggaggtaa
     4561 tttatgagga ttagtaaaaa ggtattggcg ttaggtattt cagcattact gttaagtgtt
     4621 agttttccaa catcaataaa cgcactagat aagattgaaa atattcaagg agtagataaa
     4681 tatgaaacag ctgggcttat ttcagataag caagatttta caacagcagt tcttatcaat
     4741 gcagattcta caatggtaga tgggattgct gcaagtggtc ttgcaggagt aaataatgca
     4801 gctattttat taactaataa ggatgatata ccagaagcaa cgcttcaaag actgaataga
     4861 gttactaaga tatatgtaat aggtggagaa aattctataa gtaaagatgt agaaaaaatg
     4921 ttacttatga gaagaatgca agttataaga attgatggtg tagatagagt agatacaagt
     4981 tataaaattg caggtgaaat agaaaaaatt aaaaacagtg ataagatgtt tttagtaaat
     5041 ggatttaagg atgaagctga tgcagtaagt gttgcatctg ttgcttatag agatggagct
     5101 cctataatat tgacaaaaga tataccttca gcagaagaag acacagactt agacccatgg
     5161 tttggagttt ctcctgttcc agtatatgct attggaggag agtctacatt aagtgattac
     5221 atagtgagca gatatagagc aactagaatt ggtggtgttg atagatatca aactaataaa
     5281 aatgttattg aaaagttcta taatggagca aaagagttct atataacaag tggagacgat
     5341 ttagtttatg ctttagttgc atcaccactt gcaaaaaatg ctcctgttgt tttagtttca
     5401 aataaaagtg ataagagtat attaagtgga gctagtaaag taacagcaat aggtataagt
     5461 gataagagta ttatagagca atgtttggat gctgtaaaga agtaatggag tgttattagt
     5521 aaaatgaata tacacattga aaattagtag attgttcatt ttataatggt ataaaatata
     5581 tgggaagagg ctatcaaatg gagataagcc tctttttcat tgttaaaatt atgaaaagat
     5641 actttatttt atataaaaaa gtatcttatg aactaaatgt ctattttgat atactatctc
     5701 cttttcttat attatatgat tttttatatg gaataaattt cataagtatt atcttccttt
     5761 aatttttcta accaaaccat ctctaaaata tctagttctt ctaaataatc tttctctgtt
     5821 ttgttatcat catgttttat aagttctaaa acttcaaatg taaatgcttt ttcaccatat
     5881 tttttccatt cattatttaa ttcttttatc atacatgagc cagcaccaag ctgaaatttg
     5941 agagagttaa atttgctttt tatgtcatta ctttttccaa ggtaaatctt tttagtaata
     6001 tcatttttta ttatgaatac acctgtgtca aatttcattt ctttgtataa ttgttttaat
     6061 tcacgttttc tatccatga
//
LOCUS       DACULQ010000195         6064 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.197, whole genome shotgun sequence.
ACCESSION   DACULQ010000195 DACULQ010000000
VERSION     DACULQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.197"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            190..1554
                     /locus_tag="JA154_16215"
     CDS             190..1554
                     /locus_tag="JA154_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAT7997375.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLVAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1693..2859
                     /locus_tag="JA154_16220"
     CDS             1693..2859
                     /locus_tag="JA154_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAT7997376.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2976..3758
                     /locus_tag="JA154_16225"
     CDS             2976..3758
                     /locus_tag="JA154_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAT7997377.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3877..4530)
                     /locus_tag="JA154_16230"
     CDS             complement(3877..4530)
                     /locus_tag="JA154_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7997378.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4554..5918)
                     /locus_tag="JA154_16235"
     CDS             complement(4554..5918)
                     /locus_tag="JA154_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HAT7997379.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2282 a          756 c          919 g         2107 t
ORIGIN      
        1 ttaaaaaatg tttattttaa aataaattat tttataagct tacttatact taataagggg
       61 ctttttttat tggaagattt atgttatatt gagatgaatt tattatgata taaaaaataa
      121 tgttaggata aatttaaatc tttttctaaa aatgtattaa tataattctt aaagaaagga
      181 tttttaataa tggatataaa agagttagtt aaaatgcaaa gaaaatatta taatacaggt
      241 aaaacaagag atatatcgtt tcgtatagaa cagttgaaga aacttaagtt agttgtatca
      301 caaaatgaag aaaaaatact tttagcacta aagaaagatt taaataaatc ggattttgaa
      361 ggttttatga cagaagtagg catgttttat agtgaattaa actttgctat aaagaatata
      421 agaaaatgga gcaaaataaa aagagttaaa tcaagtatgg ttaattttcc atctatatcc
      481 aaagttgttc cacaacctta tggtgttact ctcataatgt caccttggaa ttatccattt
      541 caattagcac ttataccatt agtttggtca ttagtagcag ggaattgtgt tatattaaaa
      601 ccttctgaat attctgtgtc aacatctagt gtagttaaag acatagtgga agatactttt
      661 tcaaaagaat atgttgcagt tgttcaagga agtcaagaag aaagtgaaaa gttactattg
      721 gaaagatttg attatatatt ttttacagga agtactaatg ttggtaaaat aatcatgaaa
      781 tcagcatcag agcatttaac tcctattact ttagaacttg gaggaaaaag tccttgtatc
      841 attttaaaag atgctaatat agaccttact gccaaaagac ttacttgggg aaaactaata
      901 aatgcaggac aaacctgcgt ggctccagat tatgttttag tgcatgaaga tagaaaaaat
      961 gagttaatag agaaaataaa atattatact aataagtatt ttggagataa tccatgtaat
     1021 aatgagcaat ttcctaaaat cataaatcag agacatttta atcggatatt atctcttatt
     1081 gataaagata agatagtcta tggtggaaat tataataagg agacattaaa gatagagcca
     1141 actatagtag acaatgttaa ttgggatgat aatataatga aggaagaaat atttgggcct
     1201 atatttccca ttttaacata taaagattta gatgaagtaa tacaaaaaat tatacaaatg
     1261 ccaaatccat tggcacttta tatttttaca aaaaataagt acctagaaaa taaactttta
     1321 gaaatgattc cagcaggcgg atgttgtatc aatgatacag ttactcatat agctacgaat
     1381 tacttacctt ttggtggtat tggtgaaagt ggtatgggct catatcatgg aaaagctgga
     1441 ttcgatacat ttactcacta taagagtgtg ttaaaaaaat taaatttgga tgtccctatt
     1501 agatatgctc catacgataa taaattaatt aaggttctta aaaagataat gtaggttata
     1561 ttgtagaata ataaatataa ataaaattga gtatttgtat tctgaatata ttttattttt
     1621 aatttggtat atttgttata aaagtggtga ttttgtgata aaatttaatt atatagaaat
     1681 aaggaggaaa aaatgaaaat taattggaat acaaaataca caacaatagc aatttataca
     1741 tttataatag ctgctagtag tattatattt tatctagtat catcacaaat tgatgtattt
     1801 tctaataatt tagatgctat atttacaaca ttacaacctt ttataatagg atttgctatt
     1861 gcatatcttc ttaattttat tttaaagttt tatgaagaca gaatttttat aaaaagtgag
     1921 aaattaaaaa aactaaaaca aagttcaaaa agaggcttgg gtcttttatt aacatatgca
     1981 acagctacat tgatacttta cttatttatg catttcgtat tgccacaagt aattgagagt
     2041 attgttggac tagcaaatga tatacctatg tatgtaaaca atgcaactaa gcttattgat
     2101 aaattaatga ctgatttaaa tcttgatgag caatacttta atttggcagt tgataagtgg
     2161 aatgagtttg ttacatatat tataaagttt gtaacagatt taatcccaat attaggaagt
     2221 atgttaaaga atgtagcctc tagtatatgg aatattgtac taggattaat tgtttcagta
     2281 tacttattaa ttgataaaga aaaattctat ggattaagca aaaaaataac ttatgcagta
     2341 tttacagaaa aacaagctgc cagaatttta gaattaactc atagaagtaa ctatacattt
     2401 ggaagatttt taggtggcaa aatattagat tcattcatta taggtattct aacatttgta
     2461 atacttacgt tagttaaaat gccatataca ttattaatat cagttataat tggtattaca
     2521 aatattatcc ctttctttgg acctttattt ggagctatac catctacgct aatagtgatg
     2581 tttgtatctc ctataaaagc tttctggtta ttgttgatta tattaataat acaacagata
     2641 gatggaaata ttataggacc taaaatactt ggagattcaa ttggtatatc tgcattttgg
     2701 attttgttct ccttattggt tgcaggaaaa ttattaggtt ttattggaat ggttatagga
     2761 gttccaatgt ttgcagtaat ttattctata attaaggata ttgttgaaag taaattggat
     2821 aaaaagggat taccgacaga tacttccgat tatatgtagc taaaaaatat tatgtataat
     2881 taaaaatata taaattaagc aatatttaaa gcactgagaa agttaaactc ttggtgcttt
     2941 ttagatataa aattattata gaagaggtgt tttttgtggc atttatagaa tgtaaattta
     3001 aatctaaata tactggagga gaaactgata taattgtaat tttgcctgta gcaaatggaa
     3061 aagaactttt tagtggtaaa gagatatatg attttgaagg taaatttaaa acattatact
     3121 tatttcatgg tttagaagat gaccaaagct catggattag atatactaat gtagagcatt
     3181 atgctaaaaa taaaaatata gccctagtaa taccaagagt agaaactagt ttttataccg
     3241 atatggcgta tggacaccaa tattttactt ttgtaagcga ggaattacct aaatttgtta
     3301 gagctatatt tcctttatca gataaaaggg aagataactt tgtagctggt atgtctatgg
     3361 ggggatatgg agctttaaaa ttagcgcttt caaaaccaaa agagttttcg gcagttgcaa
     3421 gtttttctgg ttcgcctgat atgattcatg agttagaaaa tcaacaaata gaaaccaatg
     3481 cagacattct atttcacgat tttggtagac cagaagacgc tagaaatagt gagaatgatt
     3541 tactttacat acttaagaac cttaaagaaa gaaatgaaga tattcctaaa atttatcaat
     3601 tttgtggtga tagagatttt ttatataaaa ataatcagat gtttaaaaaa tatgcagatt
     3661 ctctgggtgt agatgttgaa tatgaagagt gtgtaggggg gcatgaatgg cgtctttgga
     3721 atttatggat aaagaaattt atagatataa ttggatagta cacttatggt attaacaggt
     3781 atactataaa atatctttaa atatgataaa gactgaaatc ctaaaatcaa attatgattt
     3841 taaaatttca gtctctaaga atttaataaa attttattaa gttcttacac gagttattat
     3901 tggagtaaaa ttgtcaacta tatctatata gtatttactc aattcctcta aaatctcttc
     3961 gcataaatcc gtactatgac caagattaat aagaaactgt tttgtaaaat gctttattac
     4021 agcttgagaa gatatatcgt acttgtgtga agtataaact ctaattattt caggtttcat
     4081 agcaagtata ccaatacaag aaagtattaa ttcatcatgt tctatagatt ctttatcatt
     4141 aacatatctt ttcatgacca tttcggttac acttctttga tagcttaaaa tagcttcttc
     4201 ataagctata tccaaataaa aatttctata tgcttcgttt tttagaagga aacaaaggtc
     4261 agcattcata gcaagaaaga acatcatcat agtaccttct tcatggtagt tatagattaa
     4321 agactgcatt tcatttctta tatcttcata tattatagct cctatttcgc ttttacttga
     4381 gaagtaatag cttattagac ctaaatttat attagctctt tctgcaattt gcctagttgt
     4441 tgtcttatga taaccttgct caaaaaataa ttctctagca gcacttataa tactagcttt
     4501 tgtttctttg cccttttctg attttgacat atttactgtg tatacctctc ttttcaatct
     4561 ttttttgaaa atttgctaac tataaatatt gtaatagaac cacctattat aggaaccaaa
     4621 attctgtcaa atacagtaag aataccaatc atatttaata atgccaatat aacacttact
     4681 ataacagcat agatagctcc tttttttgta gcacctttcc agcaaagacc aattacaaat
     4741 ggtgcaaata ttacacctgt acataatgga gctggccaaa acaataattc taataaacta
     4801 tcaaatttaa gtgctatgat tgcacctatt atagctgtca aagctgatgc aatttgagaa
     4861 tactttagag tattttttaa atcattaaat tctatattag gatgtaataa tttgttataa
     4921 atatctcttg atataatagc actcattgag gtcattaatg aatcggcagt agacattatg
     4981 gcagccaata tagcagctaa gaataataca gatattaaag gtggcaaagt agtcgatatc
     5041 atttgaggta aaacctgaac agatgatata tttggaaata aactaccacc aatagctcca
     5101 attattccta tagggaaagc aagaaataat agtataaatc ctgcaattac actactttta
     5161 actgcatttt tagcattatc agcacattga tatcttaaaa aactttcata agaaaatatt
     5221 gagcctaaaa ctaatggtaa aactgtatac ataaaatcac taatcgattt atttcctgga
     5281 aagaatggtt cttttaccat tgattgtaac ataagtaaag ggttgtcgcc aactaatttt
     5341 aagcttatat aagctactat tggcaaaaac actatacaaa tagcaagttg aataaatgct
     5401 gatactgcaa ctccccacat tcctgatgta taagtatata taatgataac tataactcct
     5461 ataattacag ctatatcata aggaatacca aatgcagtag atacagaacc aaaagaaatc
     5521 aattgactac ccataatagc tagtatagat attggtgtta caagactttg ataagctctt
     5581 aaaaatacac tatgaccata tctagtttca atataatcag caggtgttat atcacaagat
     5641 aagcttctta attttcctgc gaaaaatgca tttataaagc aagctaaagc tgcacctata
     5701 gtagaataca ttccaaccca aagtcctctt tcagctcccc attgaactcc accaactaag
     5761 caccctcctc caaaagatgt agcagcaatg gttgcagcta gtataggcaa aggcagattt
     5821 ctacctgcga ttaggaaatc ggatgctgtt ttaacttttt ttgaaaaata aaaaccaatc
     5881 catatgtata ttgcacacgt aacaattaaa agtaacatag tcttttctcc tttataaaat
     5941 attttaataa aatatacaaa atatatttaa aatatatttt atataaaagt agtacgatat
     6001 ttgttttatt ggttttagat taatatagct ataataattt atgatattag atggaaatta
     6061 cgta
//
LOCUS       DACULQ010000196         6024 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.198, whole genome shotgun sequence.
ACCESSION   DACULQ010000196 DACULQ010000000
VERSION     DACULQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.198"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            172..1446
                     /locus_tag="JA154_16240"
     CDS             172..1446
                     /locus_tag="JA154_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAT7997380.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGINPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1653..1970)
                     /locus_tag="JA154_16245"
     CDS             complement(1653..1970)
                     /locus_tag="JA154_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAT7997381.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2237..2989
                     /locus_tag="JA154_16250"
     CDS             2237..2989
                     /locus_tag="JA154_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAT7997382.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            2976..3857
                     /locus_tag="JA154_16255"
     CDS             2976..3857
                     /locus_tag="JA154_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAT7997383.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTILSGEIMY"
     gene            complement(4083..5438)
                     /locus_tag="JA154_16260"
     CDS             complement(4083..5438)
                     /locus_tag="JA154_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7997384.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2310 a          874 c          789 g         2051 t
ORIGIN      
        1 tacagtttcc cccaactgta gctcattttc gtgtaattta ataaacatct caacaagaat
       61 taattaacaa aaccccttat aaaaacataa catatattaa acaagctctt gattaaaact
      121 tgagcttttt taataaaaaa cagaatagtt tattagataa ggggtttttt tatgcaattc
      181 atggatgtat ctaaatacat tgaaggtgca aaccttaaag ttaaaactaa aaatggtgaa
      241 actgttaaaa gaatatattt taacaactct gccactccat tagttttaaa aaacgtagtg
      301 gataacttaa actgtgagat tccttggctt acttatatta atgctccagg aataatctca
      361 gaaaaaaata ctttaaaata tgaaaatgta cgctctacta tcctcaaact tattgatgga
      421 gacgaagaaa aggattctgt tatttatgtc aaaaatgcta cagaaggaat aaacttctta
      481 gcaaaatttt ttaaagatga aaatccagat aaatttgtta ttaccacagc aatggagcat
      541 atggctaatt acctgccttt taaagttaat tttcctacaa aagttgttgg aattacagaa
      601 aaaggagaac ttgatttatg tcaattagag aatatactta aaaattatgc tggtaatgta
      661 tctctcgtta cagttacagg agctagtaat gtcacaggta taaccactcc tatatatgaa
      721 atagcaagaa tagctcataa gtatggtgca aagatattgg tggatattgt gcaagtaatt
      781 cagcataaac ctttttcaat gatgccacat gaatgtgatg agcatataga ttttgttgtt
      841 tttagtgctc ataaatgtta ttctccacta aatggaggag ctctggtagg acctaaaaaa
      901 ttctttgaaa aatttaatcc tgtactatat ggctcaggtt ctaccaaatt tgtaaatgat
      961 aacaaaatac tatatgcaaa cgtaccccaa cgttttgaag caggataccc agagtatttt
     1021 ggaactcttg caatgggaaa agcactgaac actttaaata aaattggtct aaacagaatt
     1081 tcaaaatacg aaagagaatt atttttatat gctaaagaag aaatgagtaa aatacctaat
     1141 ataataatat atggagataa atcaaataat gtaattatcc caaatatatc ttttaatata
     1201 agtaatatgt attacaaaga tgtagcaaaa tatttagttg ataactttgg aatagaaact
     1261 ggagctggag tagtaggtgc agatatttat gtacaaaaat tattaggtat aaatccaaaa
     1321 gaggcatacg tcagatttat gagggaaaat cctgtaggtc ttgttaggat aagcttagga
     1381 atgtacaata cttttgatga ggtggatagc tttatacatg ctattaaaac tttggctttt
     1441 aaataagatt tttctattct tttttatcaa taaatagaga gtagttttgt tttcacaaaa
     1501 aaacaaatta tatcaacagt caatttcatt agcaattaga cactcatttg taactataca
     1561 aactatttta cactatacaa acaaaaaact cattacaatg agtttttttg tttatatatg
     1621 agatttattt taattacttc aatttacttt aattacatat atgattcaat tttgtttttt
     1681 aaattctcct taggcataaa acctattaat ctatctacag gttttccatc cttaaatatt
     1741 agcatagttg gtactgtaga tacttcaaat ttctgagcta attctatact ttggtctatg
     1801 tccaccttca agaagtttgc tttttcaacc atctcatttc ctaaagcttc atatattggt
     1861 gatagcatct tacaaggtcc acaccaagtc gcaaagaaat ctacaactac taaaccatct
     1921 ttattttcta cttcatttat aaattcatta ttatttatta tttttaccat aatatacatc
     1981 tcctttatta tcttcttgat attaatatac tacgccctat ataacagttc aattaggcac
     2041 ttttttgtaa ccgcctattt ttaatagatt taatattatg ataaatagat ttataagatt
     2101 aaatatttat gagactaaat atttctaata tattttattt ttttcttaat atagttgaca
     2161 ttttttccct taaattataa aatcaaatta ataactacat aggtagttac taatctttta
     2221 tcaaaggagt ttctatatgg ataatataat taatgaatta caaaatatag gatttactaa
     2281 atacgaatct caaatataca tatcattatt aaaggaaagc cctttaacag gatatgaaat
     2341 aagtaaatta tcaggtgtac cacaatcaaa agtctatgaa aatataacaa agctattaaa
     2401 caataatata attatcaatg ttggaactga ccctattaag tacataccaa taaaccctaa
     2461 tgaattatta aaaaataaga aaaaagaatt tgacaattct atggataatc taaagaatag
     2521 cttacagata ttaaataaaa gtaaaagtgt agaatacgta ttaaacataa aaggagataa
     2581 aaatatatta aaaaaagcaa tagaaatgat aaaaaatgca aaacaagaaa ttataatttc
     2641 ttgcccttat gaagaaattc ttgaattaaa aaaagaactc ctaattgctt acaaaaaaaa
     2701 tattaatatt gaaatactac ttttagaaaa caaaagtctg aaaggtcttg ataatattca
     2761 tattcatgga ggtaataaaa ctacacttga aaataaaatg ctttacaaag gaattatttt
     2821 gattgtagat aatgatgaaa tattgacagg aaacttatca gaaggcagtg aggccatttc
     2881 aatttggaca aaaaactcta acattttata tattggagtt caatacataa aacatgaaat
     2941 ttatatttca gaacaaataa aaggaggtta taaaaatgaa caaaattaag attaaacaag
     3001 ttgatgcttt tacaactata ccttttggtg gaaatcctgc tggtgtcgtt actgatgcct
     3061 ctaacatttc tgatgaaatt aagcagaatg tggctaaaga aatgaattta tcagaaactg
     3121 catttgtttc agagtcaaat attgcagatt ttaaagtaca atttttcaca ccaggtttag
     3181 aagttgattt atgtggtcat gcaacaatag gtactttcca tgcacttttg gaagaaggga
     3241 agcttaatac taataagagt atattttatc aagaaacaaa agctggtgta ttatctgtag
     3301 agcttaagaa aataaataat gaaaatatat ttatgatgga acagaataca cctgaatttg
     3361 aaaatttaga taattgcaga aaagaacttg cacaaatgat aggtgtgcat gaagatagac
     3421 ttcttgaata tcctattata aaggtaaata ctggactttg gtggcttgta tttggtctaa
     3481 aatctttaga tgatttaaaa aatatctctc cagatttaaa taaaatcaaa aaatttagtg
     3541 gtgataataa tataattggt ataactccat tttgtataga aactatagat aaaaagtgtg
     3601 actaccattt aagatctata gcaccatatg ttggagttac agaagaccct gtatgtggaa
     3661 ctgggaacgc ttgcgtatca tcttatatag tacatcacaa tattttagat aaaactagtt
     3721 ttattggtga gcaaggtaac tttataaaca gacctggaaa ggtatatgta aatataacta
     3781 gagaaaacaa tgacataact tctgtaaaaa ttggaggaag tgcttatact attttaagtg
     3841 gtgaaattat gtattaatcc catacctaat aaaaacaaat aaaaatacta gctaactaaa
     3901 gctagtattt ttatttgtta tttttattta ataatcatac tctaacaaat attataatta
     3961 tatatatcat aatattgtat ttagaagtaa aattttattt ttattattaa ttagttataa
     4021 atcaatctaa tacaacataa ttacagtaga ttaacctata aaacataata attgcttact
     4081 aattatttat catcctcaac aagtctactt aatgtcttat cagataagaa taataatatc
     4141 gctgcaacaa atagtattac tggaattata gtatatactt taatcatatc aaatttaaga
     4201 gtaaatgcat atatatatcc gtatccttta ccagctataa aagtagcaaa tgtccatact
     4261 cccataagaa cacctagaag cttcttaggt gcatacttac ttacaaaaga gttaccaagt
     4321 ggtgagaata acatttcacc aagacttaaa agaataccaa acatgattat ccatagtata
     4381 ctagctttag cagtttcagg agctccaacc cctcttgaaa attctgcacc aacaagcatt
     4441 aagaatgcaa gacctaagaa tattaatcca agacctgtct ttttaaataa actcatatct
     4501 ccttgtggtc tactagcaag tttaaaccat aatgctccaa gaactggtcc taatactata
     4561 catacaagtg aatttaatga gtcaaaccaa gcaagaggaa catcaaatcc tccaacattc
     4621 atgtttacaa atgctgcacc ataatcataa actgcaagat aagttagata ccagaatacc
     4681 cagaaaatta cagagaagaa tgatataagt ataatagccc aaattctctt cttatccatg
     4741 cttgtaagag gagacttatc tttttcttca attttttcag caactacacc ttctttaaaa
     4801 ggtctctttc ctgcattccc aaggaatctc catccatata tgaaccatat tgcacctatt
     4861 acacaaagta cagctgctat aaagaaacat tgagaaaatc caagtacacc attttttgca
     4921 aatgttttaa ggtaaagaat accaactgct atagtaccaa taaaagaacc tatattaaca
     4981 aaagaatact gtactgaaaa tgctgtatca agttcttctt gactatcaaa aagctgtcca
     5041 tttaccgcag atacgttacc tttaaagaat gcagtaccaa ttgaaactaa gattatcatt
     5101 gcatttacca tagaaactgt agttgcaata gaaccaagat agtatcctac tcccatgata
     5161 agcataccta taggtatagt atatctagct cctatccatc tatctgatat atatccccca
     5221 attataggtg caaggtatgt gaatgcaaca aggttggctt gcattatagc agcttgtcct
     5281 ttatctattc caagaccacc atgaactata gctgttgtaa ggaatatata tattaagtac
     5341 tttgaagcat aatatgctgc tctttcaaat gagaaagttg ttgagcagat ataaaatccc
     5401 ataggaaatt tacccttctt ttttactgtt gagctcataa ttttaaatcc tttcttaatt
     5461 gtatttttat tttttacgtt ttgtccaaat tctttctttt atgaaagata taatattatt
     5521 attttgtaaa acaatttgta attattatta agataattgc gcatttattt gtagagtaca
     5581 tattctcatg gaataaaatt aatacacttc ttaatcataa atttaaatta tataaattca
     5641 ctattttcag atatagttat aaattaatat attctcatct aataccatga aaaaaccttc
     5701 gccatatgca attcttattt tttacgtgat agtattttac aaactaaaat cccccttttg
     5761 tagcaactta tgtaattaca ttttagtgct tttttaaagg agtctcgaat aaagcaacat
     5821 atattttgtt caaataatgt ttttatttat tttttaaatt tatatattac ttcactcaac
     5881 caattctcaa cttaaataac taaactacta tctattcaca gattaggctt atttcttaca
     5941 cattagttta taataaacac tgtgaatcta aacataaaaa ctgttatttt aaagtaatta
     6001 cccatatatt cgctttgata ctat
//
LOCUS       DACULQ010000197         5970 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.199, whole genome shotgun sequence.
ACCESSION   DACULQ010000197 DACULQ010000000
VERSION     DACULQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.199"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..442)
                     /locus_tag="JA154_16265"
     CDS             complement(<1..442)
                     /locus_tag="JA154_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAT7997385.1"
                     /translation="MVENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANN
                     LVDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKE
                     LIYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLS"
     gene            complement(675..1892)
                     /locus_tag="JA154_16270"
     CDS             complement(675..1892)
                     /locus_tag="JA154_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT7997386.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(2092..2955)
                     /locus_tag="JA154_16275"
     CDS             complement(2092..2955)
                     /locus_tag="JA154_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAT7997387.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(3037..4959)
                     /locus_tag="JA154_16280"
     CDS             complement(3037..4959)
                     /locus_tag="JA154_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HAT7997388.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(5023..5871)
                     /locus_tag="JA154_16285"
     CDS             complement(5023..5871)
                     /locus_tag="JA154_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAT7997389.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
BASE COUNT         1918 a         1157 c          697 g         2198 t
ORIGIN      
        1 tactcagata aaatacgtat tctccagatt tagaaaatgt atctacaact tctcttaagt
       61 tttctacatt tagatatttc tcacttaaaa ctaaccctag ttttacatac tcaacttttt
      121 tactcttata tattaattct ttagttaatt tatataacca tttctcatca ataatcattt
      181 cttttaccaa agtatttatc tttttcataa aaatttcgaa ataacaaatt aaagacttag
      241 aattactacc ctccaataaa taaatttcat tagattcact tttatacata tttaaaattt
      301 catctactag attatttgca agctctactt gttctcttaa cggtatactt tcatatatac
      361 ataggtaatt catttcaaat gattctttgt atccatcata accattaaat tcctgcaatc
      421 gcttaaaaac attctccacc aaattatcct cctttacatg atgtaattta tattgattat
      481 actacttatt tcatcaagtt tacatttata cgcaaaaaaa tattactaga aattgaaatt
      541 ctagtaatat ccttattatt aattatgtaa ataccctatc ttataactgt aaataatatc
      601 cttattaatt ttaattactt ttaataaaaa tctatcctag atttagactt ctctatgcat
      661 atattatact aatattagac atcataatca tacacttttt ctttttcata tctcatgttt
      721 acaaacaatg caagtaaaat tccaacaaat gttattgcca cattaaacaa tacaacatat
      781 tttggtccat taactccccc tgattttgta ataatacctg ctatatttaa aataacatag
      841 tttgctatac tagatgcaat cataactata gatgttattt ttcctttatt agttggaaac
      901 atttcatttg cagttgacgt tgttaactga agaacacctc ctgctgctga ataacctatt
      961 acaaatcccc ctaacataca aatagttgga gtttgaacaa aatatacaac taatagcata
     1021 agtaaagcta ttataggata tataactaat attcttattg gtttaatata ttttttaata
     1081 agtaaagaag ttattaatac tgcacatatt actcccattg agtaaaagga ttgaattttt
     1141 gtagggtcag ccatcccata caatttaccc aattcctgat tacaatttaa ccataataca
     1201 aatgtagatg tacatgtaaa tccaatacaa acaagagcta tacttgttgg tgtaaatttc
     1261 atcttctctg atttaactgt tttgcctttg ttatcaataa cattattagc tggtggaaat
     1321 ggtaacacag ctatcaaaaa ggcatctact acaataagaa tcgctgttac tataaaaata
     1381 actttaaatg atattgaatt tgctgcaaca aatccaatta taaaaggaag caaaaattgt
     1441 gctaaagcta ttgtaaattt agtaaacata tttgctattg ttcctaaact agcaaatatc
     1501 tccatacaag aaggagttac acaggtatct aagaatgaat ttgctgcccc tccaattatt
     1561 gcaaatacat atgctatata aaaatttggt gaaaatacaa ttccaacaaa gaaaattgca
     1621 tgtagaaagt ttcctataag accacttact tttcttccat acttatctga aataggtcct
     1681 gcaaatggat aagatataag tctccctaat cctagtgcag caataacagc taaaacaata
     1741 cttacatcta atgttccatc tgataatgtt ttagcccccc acattgatga aaattctggt
     1801 ttgtattgtc ctaaaattga aattccaata ccaagtaaaa aatatgaaaa atacaacgct
     1861 atggcagtag gataatattt tttattgttc ataatttttc tcccattctt gttaatttat
     1921 taacattttt gtgtacaata aactcatccc cataatggat ttaaagtata gtcagttaaa
     1981 acttcaaatt taactggact atgaggcaaa acccactatg acaatttgta atttattatt
     2041 aaaaatattg aaatacgtat gttttttcat tgtttaagat attactctta tctatttaaa
     2101 gcataattca ttaacttctt ctacaggcat ttctaatcca gtatatagat taaaagcttc
     2161 tgctccttgg tataataaca ttcctaatcc accaatagcc ttacatccat tagcttcagc
     2221 atcttcaatc atttttgttt tcttaggatt ataaacgaca tctgttacta ctaaatcttt
     2281 tcttaaaaca ctagtatctt ttatatttgt ttcattgtca taaggcttca taccaataag
     2341 agttgcattt gtcaatatat cacttgattg tatctcctca taaagtttgt ttgtatcttc
     2401 taaatcatat agattaacaa cacattctgg tacatctttt tttatattct caactgtttg
     2461 ctctgctctt ttataaaaat catccttagg attaaagata gatatttctc ttgctccatc
     2521 taaagcacat tgtacttgta tagcagttgc tgcaccacca gcacccataa ttgtaatctt
     2581 tttaccttta acttctaccc cattttcttt taaattacga acatagccta ctccatctgt
     2641 tatatgacca actaactttc cattgtcatt tacaattgta ttacatgctc caattatacg
     2701 agctgctgga gaaagttcat ccatatattc tgtaacagca gatttacatg gcatagtaac
     2761 attggctcct tttatattaa aagtttttat tgctaaaagt gcctctttta ccttatctac
     2821 tgtgatatca aaggctaaat atctataatc taaatctaat ttcttaaaac tataattata
     2881 catcactggt gatttagagt gaccaaccgg tgtacctatt aatgcaaata atcctgttct
     2941 acctgaaatt tccataacta taatctcctc tcaatattat cattttcttt gcaaataacc
     3001 tattcagtta tttacaaaga aaaatttatt tattatttat atatttaatg ctacttcaaa
     3061 agcttctttt gttgcatcta atgccctacg agcttttact gcatcaccaa caatataagt
     3121 ttcttttaca acttctttta taacttcact taatgggtcg taattacgag aacccatagc
     3181 aagtactact gaatcaaaac cttttaaatc atgctctgtg ccatctgtaa gtaaatagtc
     3241 gacaccatca tcataaaatt tagaaacttt tgcaccagta atggtatgta tattataatt
     3301 ttcaaaatca ttcataagga atttacggtg ttctgaaatt acatctgcac caacttcttc
     3361 tctaagctca ataacagtaa cctcatgttc ttgttctcca aggaaggctg ccacttcaca
     3421 tccaaccatt ccacctccaa caacaagtac ttttttccca caagattttt taccatcaag
     3481 aaggtctaca gcatgaatta atcctgaatc attgattcct ggtataggaa gtacaagtgg
     3541 agttgctcca gtagctataa taactacatc aggtgatttc tcctttataa gttctactgt
     3601 agcttctgta ttagtacata tttttacacc atattgatta cattttgaaa tataacttct
     3661 aactaagtta gtaatatctc ctttccctgg aggatatgca gctaaacgca tttgcccacc
     3721 aagaacttca gatttttcaa ctaaagttac attatgtcct cttttagcac atacccatgc
     3781 tgcaagcata cctccaacac ctccaccaac aacaagaact tctttgctaa tttcagctgg
     3841 cttaagctct gcttcatgtc ctaatatagg atttgcaaga caggctatag gtttgccttg
     3901 gaacatattt ggtacacatc cttgtaagca tgcaatacat ggtatcattt cgtctagttt
     3961 tccattttta gctttattag gcatttctgg gtcagccaag ctttgtctac cgaatgctac
     4021 taaatcacat cttccttgtc tgaccattaa ctcagcaaac tgtggctctg tataacgtcc
     4081 aactgttatt actggtatac taacagcttt ttttatttca gtaactaatt ctgcactgaa
     4141 tccagcatgt aaagtcgtag gagcccacat atattcatca tgtatatgta tactacgtga
     4201 aacatgaagt ccatctattc cacaatcttc tagatatgct gctattactg aactatcgtg
     4261 tacatcaatt cctcctggaa catcatcagt gctgtttata cgacataata ttgctagaga
     4321 atttcctact tttttacgta tattttcaat gataagacga ggtaatctca ttctattttc
     4381 aaaacatcca ccaaactcat ctacacgctt atttgttctt tgtgatataa aactactaac
     4441 cagatatcca tgagcgcagt gaacttcaac agcatctgca ccagcttttt gtgcacgtaa
     4501 tgcagcatct ccataagatt ctataagttc atatatttct tctgttgtta ttgctagtgg
     4561 tgtattacga ccatttgatg caggtattgc agaagcagct tttaatggat gtcctgctac
     4621 tttttcattt ccttcagaac cagcatgttg taattgtata gataccttcg aacctgcatc
     4681 atgacaagca tctattacat ttttaaaact atctattgtg ctatcatcat ataaacaagg
     4741 cttacgagga cctccttttg cttttttgtc tacaacacta gattctatag ttattaatcc
     4801 aaaaccacct aaagcacgct caagataata agccttagaa gtttcactta aagtaccatc
     4861 tgtgtttgca aaattgtttc ccataggggg aactacaaaa cgatttggaa ctgtcattga
     4921 accaattctt ataggtgtaa acatagaact aatcttcata aggttttcct tcctttcaag
     4981 ttttatatta taatactctg tatattttta tattatataa gtttattttg gagatacttc
     5041 agaccatgct ttatccaaaa cttttttagt tgcattaaat acagtttcag cagcagtgga
     5101 taacccttta gatattagag aatcagatat tacttctgcc cctatgactc ttggtgatat
     5161 attgtgttct tttattattt tcacaaattc ctctgtgttt ccatatcctt cacctggagc
     5221 caaacggtca tgtagtgatt catccctaag tttttcataa ggagtttcta aaacatcaca
     5281 cacttgaatt gatactatct tatctgctgg aacaggtgca aggtcctctg ctgtctgctt
     5341 tgctctagcc cagtgccaag tatctaatat aagcattgca ttttcacgat tacatccttt
     5401 cacaacacgc catgcagtag ccaaatctgg tacaccacta tatggcataa actcaagacc
     5461 tataataagt tcacctgctc tgtcacataa ttctcccaat gcagttataa tttgttcttc
     5521 tggtatcatc tctaataatc cacaatttat atgtttaaca ttaaataagt gagccatatg
     5581 ataaacattt tgctcttttt ctctttgagc ttccgttcta tcttcttctc ttccccattg
     5641 agttatatat tctacttctg taactttcat atcatattca ttaagaattg caatcatttc
     5701 ttcatctgta acacctgcat tttttgcatc aatataattt tcagcacgca atccaatacc
     5761 atcaaaccca gcttcttttg caatacgaac tctatcacgg aatgaaactt ctgttcccaa
     5821 tgtataagaa ctaattgtaa ttggaatatt aagatttttt tgattattca tagcttaaat
     5881 ttgccccctt tattttcttt ataattaata gtatttttga ttttatattt ttatttatcc
     5941 tactagtagt attattttac cttaaaaata
//
LOCUS       DACULQ010000198         5968 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.200, whole genome shotgun sequence.
ACCESSION   DACULQ010000198 DACULQ010000000
VERSION     DACULQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.200"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            2..655
                     /locus_tag="JA154_16290"
     CDS             2..655
                     /locus_tag="JA154_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding protein"
                     /protein_id="HAT7997390.1"
                     /translation="MMKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFI
                     IEGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNV
                     FEEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKA
                     IRNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(795..2171)
                     /locus_tag="JA154_16295"
     CDS             complement(795..2171)
                     /locus_tag="JA154_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT7997391.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2187..4100)
                     /locus_tag="JA154_16300"
     CDS             complement(2187..4100)
                     /locus_tag="JA154_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT7997392.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4236..4868
                     /locus_tag="JA154_16305"
     CDS             4236..4868
                     /locus_tag="JA154_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAT7997393.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5007..5546
                     /locus_tag="JA154_16310"
     CDS             5007..5546
                     /locus_tag="JA154_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase"
                     /protein_id="HAT7997394.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(5722..5892)
                     /locus_tag="JA154_16315"
     CDS             complement(5722..5892)
                     /locus_tag="JA154_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAT7997395.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2202 a          842 c          841 g         2083 t
ORIGIN      
        1 tttgatgaaa gcagaaaaaa caagtatata tccaattatt aaaaataatt taaacaaaat
       61 atcaattgtt gaatataaaa agggtcaaag atttgtagca tcagataaag acttacaaga
      121 agtgttcttt ataatagagg gagttgcatt agtggagtgt actactagag gaggaaataa
      181 atttcttgtt gacattgtac ctgaaaatga atttataggc aaaattagtt acatatatga
      241 acataatttg aaatgtgata tatttgcaaa gactaatata aaattatttc gatttgaaaa
      301 aaatgtattt gaagagtttt atctcaaacc agattttatt gcactatttc acagaaaatg
      361 tacaagaaga atatatgaat tatataaaac cagaatggta agagaactat tttcttgcac
      421 agaagttatt gcctattgta tatcaaataa acaagaaaat aatgtctgca atataaaagc
      481 tattcgtaat ttcagtgaaa tctattcaat tagtagaaaa agtagatacc attctctgct
      541 aaagttaact gaaaaggaga tagtaaaaaa aatgggtaat acatatgaaa ttttaaacta
      601 tgatgaatta cataaattgg catttgaagt aaaagaattt ttggaagatg agtaaaaatt
      661 gttacttaat caaaataaag tataataatt tctgtaaaaa atagaaatag tctcactgaa
      721 aatcaaaaat actttagtta ttttcaatga gactgtttag aaatattgta tacttataat
      781 gtatagtata attattaatc aatttccttt actatattaa tagcccattt tttagataat
      841 gctctaggaa caccaaatat aaacttaacc acttcttcac tataagtgag caaaacgagt
      901 acaaatatag gtgcaccttt aaatgcagct ataaaagtta gaggaataga agctaaccac
      961 atacatccta attctaaaaa tagagcatat ttagtatctc ctccacttct taaaatacca
     1021 attataacca atgtattaaa agatttaaga gccattagta ttcccattat aaagaatatc
     1081 ttagttatat cagaagctaa gctatcagaa acactaaaca tttttaataa taatggtata
     1141 tttaaaatta aaataattcc aagtattaaa ccagctgaaa atacaagtat agaaaacttt
     1201 ttagcatatt caatggctct tttaatatgg tcagctccaa gttcatttcc aagcataata
     1261 gatgcaccag atgcaataca tacagctgtc attatgaaga aattacctgt actggttgct
     1321 atctggctag ctgctatagc agaagtacca gctcttgcat atgcaacaga atacaggaca
     1381 gttccaattg cccatacaga gtcatttaca aatataggaa agctttttga tgaaaaagat
     1441 tttataaaaa ttttatcaat agcttttaga ttttttagac caaactttaa tatatagtct
     1501 tttttataaa ggtaaacata agtaatcatt aaaattaatt cacatattct agcaataaca
     1561 gtagctagag cagcaccttt tacacccaat gcaggaagac cgaagttacc aaatataaat
     1621 ccataattta aaataacatt tgttacaagt gcaaatgcac tacaaatcat acctaactta
     1681 ggatttctaa cacctcttga accagtacta aatacagtac ttacagcaat taatggataa
     1741 ctaaatactg caatactaaa gtaatctata caaagtttta ccacctcaga atcatacgaa
     1801 aatatgtgta taattggctt tggtgttagt aaagcaggaa taacaaatac aaaacttaaa
     1861 acaattgcca atacacaagt aagacctgtg acttttctta tattactcac atccttcttt
     1921 ccataaaact gtgaaataaa aactccagca cctcctgtga taccagataa ggacatatta
     1981 aaaagaaaga aaaattggtt tgcaactcca actgaagcta cagatgtttc ccccaaacta
     2041 cttatcataa ttgtatctat tacatttaca gaagtagaaa ttaagttctg tatgataata
     2101 ggtatacata aagatagaag tattttataa aatcttttat tttcgtcttt taaagaaaat
     2161 agttcttgca aataatcctc tcctttttac ataaattctt cccactcatc atatagagag
     2221 gcaaggttat tttctaaatt aattttttct tggctaactt ctttagcttt atctggatta
     2281 gagtatactt cttcttgaca taaaagtata tctaattttt caatttcaat ctctaacttt
     2341 tcaatttctt tttctatatt ttgtcttttt attctatttt tcttttctat ttctctttgt
     2401 tctctctctt ttcttttttc atcttttatt tgagttctag ttttttcttc tttttcttct
     2461 ataatactca tctcttggat ttgccttttt ttatctatat aataatcata atttcccaga
     2521 tattcagtaa ttccattttc atctaaaact aaaattttat caacaacagt atttagaaag
     2581 tatctatcat gtgatattgt aaatatagtc ccatcataat tagttaaggc ctcttcaagg
     2641 acttccttag aatcaatatc taggtgatta gtaggctcat ctaataacaa taagtttgca
     2701 tttgatagta taagtttaag tatggcaact ctagctcttt ctccaccact caatgtagaa
     2761 atcttcttaa aaacttcttc atctacaaat aaaaatgccc ctaacatatt tctcaaagta
     2821 gtttgagtaa gatgagtatt attttcccaa atctcatcaa ttattgtgtt atctaaatta
     2881 agagtttttt gttcctgatg gaagtaggaa acatgtacat tagttccaaa tttaatattg
     2941 ccactaagag gttgtagttc attagttatt attttaaata atgtagattt gccaacacca
     3001 tttgcaccta taagtgcaac tttttcccct ctatatatat ctaaatccaa gtctttaaaa
     3061 agtattcttt ctccataacc catagataca tctctaattt gaagaacatc attaccactc
     3121 tggacagaag gcgtaaattg tatacgagct tttttcctat aagcttctgg tttatctaaa
     3181 acatctactt tgtctaatgc tttttcttta cttcttgcac gctttaggtg tttttctcta
     3241 ccataagctt ttagtctttc aattgattct tcttgctttt ttatttcttt ttgttggtct
     3301 tcaaatttct taagctctag ttctttttct attttagata actcaacaaa tttagagtag
     3361 ttaccattat atactttaag ctttttattg tgaacttcaa aaattctatt tacggattgg
     3421 tctaagaaat atctatcatg agatattaac atgacagtcc ctttatattg ttttaaaaat
     3481 acttctaacc attctattgc ttcagagtct aagtggttag taggttcatc aagtaacaat
     3541 agagttggtt tcttaagaag taattttcct aaaagaactc tagttttttc accaccagaa
     3601 agtatgctta ttggcttatc catatcattg tcactaaatc ctaatccctt tagaactcct
     3661 ttggcttcag atttatatcc atatccattt aaatctgaaa ataattctag tttatgtgaa
     3721 tattcatcca tgattttttc tataattgga gagtttgttt ttgtactttc ttcagatatt
     3781 ttaacctcca attctcttaa ataggattcc atttctatca aatttttaaa aacttctagt
     3841 acttcttcca aaatagtatt atccgaataa aaattggtat tttgttctaa atatccgatt
     3901 tcacaatctt tagaagtgta aatatctcca ctatcatatc cataaattcc tgaaattatt
     3961 ttaaataaag tagtttttcc agtaccatta acaccgatta taccaatttt atcgccttca
     4021 tttactgtaa aactaatatt ttctaatata gaatctatac caaaactttt atttaagtta
     4081 ttacatgaca aaacaatcat aaatttctac ctccttgaac tatctaaaat tataatgttt
     4141 tgatataata taatcaaggt tatatgtatt agttttgaaa aagaaatatt ttattgtgaa
     4201 tcaaacaaaa aagaggacac ttttaggagg tgaagatgtt gggaaataaa aatatatcaa
     4261 tggcagttat aagaaggctc ccaaaatatc atagatatct tggagactta ttagataggg
     4321 atatacaaag aatatcttct aaagaattga gtgatataat agggtttacc gcttctcaaa
     4381 taagacaaga tttaaacaac tttggtggat ttggacaaca aggatatggt tataatgtag
     4441 aagctcttca tactgagata ggtaaaattc ttgggttgga tcgaccatac aacgcagttc
     4501 ttgtaggagc aggtaactta ggacaagcta tagccaatta tgcaggattt agaaaagctg
     4561 gattcgagat aaaagcttta tttgatgcaa atcctagaat gataggttta aagataagag
     4621 agtttgaagt attagattca gatactttag aagactttat aaaaaacaat aatatagata
     4681 ttgctgtatt atgtatacct aaaaatggag cacaagaagt tattaataga gttgtaaaag
     4741 ctggaatcaa aggtgtatgg aattttgcac ctttagattt agaagttccg aaaggtgtta
     4801 tagttgaaaa tgtaaactta acagaaagtt tatttacctt atcgtattta atgaaagaag
     4861 gaaagtagac aacttaaaca tattaataat gagcagttgt aaagagtatt gagaaagtca
     4921 gaagatttaa tattaaatcc tctggctttt tgaaatatta ggataaaaat ttaaataatg
     4981 tatggaaata aacaatatta aggagagtgg tttttgtgga ggaattatgg aaaaaggcaa
     5041 ttgaatttca tggtcatgaa tgtccagggt tagctatagg atttaaagca gcacttgcag
     5101 caaaaaaata tttaggatgt aaacagtcag gagatgagga aattgtatgt atttctgaaa
     5161 atgatgcttg tggaatagat ggtatacaag tagtgctaag ttgcacaatg ggtaagggaa
     5221 atttactact taggatgact ggaaaatcag tatatcattt ctttaataga gaaaatggaa
     5281 aatctataag gatatatgta aagtcaaaac catttgagat ggatagagaa ggatacaaaa
     5341 gatatttatt agaaagtgat tttgaagaaa tatttgagat tacagatact aagttaagac
     5401 ttcctgaaaa agcaagaata tttaaatccc ttaaatgtga agtgtgtggt gaatttgcat
     5461 ctgagcaata tataaggatt gaagaaggaa agaaagtatg tcttgattgt tttgataaat
     5521 atgaaagatt ttatgaagag tattaaaatt ataaattaga ttaaagttaa aagtattaga
     5581 tatgtgctaa cagtatttta agatgaatta ggtagcaaca taataaagta tataaataat
     5641 attaattgaa tcatacataa ttaaaaagag gctgattagt atttataaaa taaaatcagt
     5701 ctctttatga ataacgaatt attattctgg ttgaggtgca tctacaggac aagctcctgc
     5761 acaagaacca cagtctatac aagaaccagc atctataaca tatgcgtcgt ctccagctga
     5821 tatacagcta acagggcatt cagcctcaca agcaccacag cttatacaag cgtcagtaat
     5881 tttatatgcc attttgaaat cctccctttt atatgtattg taattcaata acattataac
     5941 aaatattatt gaatataaca attaaatt
//
LOCUS       DACULQ010000199         5933 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.201, whole genome shotgun sequence.
ACCESSION   DACULQ010000199 DACULQ010000000
VERSION     DACULQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.201"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(200..2032)
                     /locus_tag="JA154_16320"
     CDS             complement(200..2032)
                     /locus_tag="JA154_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997396.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVE
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2025..4271)
                     /locus_tag="JA154_16325"
     CDS             complement(2025..4271)
                     /locus_tag="JA154_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997397.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4339..4800)
                     /locus_tag="JA154_16330"
     CDS             complement(4339..4800)
                     /locus_tag="JA154_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7997398.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(4973..5095)
                     /locus_tag="JA154_16335"
                     /pseudo
     CDS             complement(4973..5095)
                     /locus_tag="JA154_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589406.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5105..5671)
                     /locus_tag="JA154_16340"
     CDS             complement(5105..5671)
                     /locus_tag="JA154_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HAT7997399.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1891 a         1085 c          637 g         2320 t
ORIGIN      
        1 taaaataaac actttgtaaa gacataaaat tatgtggcta tctcaaaata tagagaatcc
       61 tctttttcat tgttaaaatg ataaaaatac actttatttc atacacaaaa gagtgcctta
      121 tgaattaaaa agttcatttt aaggcaccct catttatttt atcttattaa ttacatgtct
      181 atgctaagat agttttttgt tacattgctt catcttcttc aaattgagaa ttgtaaagat
      241 tggcatagaa tccgcctttc tttagaagtt cctcatgatt tccttgttca actatatctc
      301 cttcattcat tacaagaatc atatcagcat cacgtatagt tgataatctg tgggctataa
      361 caaaacttgt tctaccttcc ataagattat ccatagcttt ttgaatcaat acttctgttc
      421 tagtatcgac agaacttgta gcttcatcta atattaaaat tttaggatca gctaaaatag
      481 cacgagctat agtcaataat tgtttttgac cttgagaaac attacttgct tcttcattta
      541 atttcatctt atatccatct ggtaatgtct taataaatct atgaacatgt gcagatttag
      601 cagcctctat aacttcttca tctgtagcat tcaacttacc atatcttata ttttccataa
      661 ttgaaccact aaataaccat gtatcttgaa gaaccatacc aaacatttct ctaagctcac
      721 tacgattaaa atctttaata ttatgaccat caatcagtat tgaaccgcta ttaacatcat
      781 aaaatctcat taacagttta actattgtag ttttacctgc tcctgtaggt ccaacaatag
      841 caactttttg accaggttca acatttactg agaaatcatt tattatagtc ttgtttgggt
      901 tatatccaaa gttgacattt tcaaaatcaa ttttaccttc taaaccatct attgaaactg
      961 cattttcaac aatttggact tcttcttcct cttctaaaaa ctcaaatact ctttcagaag
     1021 ctgctgctgt tgattgcaat aaattggcaa cctgtgcaat ctgagtcatt ggctgtgtaa
     1081 agtttcttac atattgtata aatgactgta tgtcaccaac ctcaattgtt ttcttaattg
     1141 caagccaacc tcctaagatt gataccatta catatcccaa gtttccaata aacatcataa
     1201 gtggttgcat tatacctgat aaaaattgag atttccacgc tgaattatac aggctatcat
     1261 ttagtttatc aaactcttca attacttcat cctcacgatt aaatgctttt actattgttt
     1321 ggccactgta agtttcctca acttgaccat ttacatttcc taaatattct tgttgatatc
     1381 taaagtattt ttgtgatttc tttattattc ttgaaattac aaacattgat attggtaata
     1441 ttaaaactgc cactaatgtc ataattccac ttatagataa catcattatc aaaacaccta
     1501 tcatagttgt tactgatgtt ataagctgtg tcatactttg atttaaactt tgatttaaag
     1561 tatctatatc attcgtaatt ctcgataata tttcaccatg cgtcttagta tcaaaatact
     1621 tcattggcaa tctatccaat tttgctgaaa tctcttttct taaattatag gatacttttt
     1681 gagaaattcc actcattata aatccttgta taaaagagaa cagtgcactt atcaaataaa
     1741 ggaaaagtaa aaaggttaag atttttccaa ttgcattaaa atctatacct acgtttcctc
     1801 cagaaacttt actcactagc ccttcaaata tctttgtagt tgcctttcct aaaatttttg
     1861 gacctactat agaaaacgac gcactaccaa tagcaaatac aattactaga attattgata
     1921 ttttaaattt agataaatat gcaattagtt ttcgcatagt ccctttaaag tcatttgctt
     1981 tttgtcctgc tcccatagat tttcccatag gacctcttcc cattctactc attagctagc
     2041 tcctcctttg aaagttgtga taaagctatt tgctcataaa cttcgcaatt ttttagaagc
     2101 tctttatgtg ttcctattcc aactatttcg ccctcattta aaacaattat ctgttctgca
     2161 tttaagatag tactaattct ttgtgcaact attagaactg tactttcagc agtttttgat
     2221 tttaaagcct tacgaagagc tgcatcagtt tttaagtcaa gtgctgaaaa actatcatca
     2281 aatatataaa cttctggatt ttttgctatt gctcttgcaa tagaaagtct ttgtttttga
     2341 cctccagata catttgttcc accttgagaa atttcagttt taaaagcttc cggtttagaa
     2401 tctataaatt ctgtagcttg agctatttca gctgctgttc tcatttcttc atctgttgcg
     2461 tcttttctac catattttaa gttggattct atagtaccag aaaacaatac tcccttttga
     2521 ggtacatatc caattttttc ccttaaatca tgttgagaaa cattttttat attaactcca
     2581 ccaagtaata tttctccttc agtaacatca aagaaacgag gaattaaatt tataagagtt
     2641 gacttaccac taccagtact tcctataaag gcagttgttt ctcctggctt tgctgtaaaa
     2701 gatatatttg ttaatatagg ttcttctgcg tctggataca tgaacgaaac atttttaaat
     2761 tcaacaagac cctttttatc ttccttaaac tcttgtgcct ctgatttttc tgggtctttt
     2821 atgcttatat ctgacactaa tacttcatct atacgtgtag ctgaaacgga tgctcttgga
     2881 agcataatag ataccattga tatcattaag aatgacatta ttatttgcat tgtatattgt
     2941 ataaatgcca tcatatctcc aacttgcatt gctccagaat caacactgta tgcacctctc
     3001 caaactataa gaactgtgat aaggttcatt ataagcatca tagcaggcat catacatgtc
     3061 ataactctat ttacaaaaag atttgtttta gttaaatttt tatttgcttt atcaaatctt
     3121 tcttcttcat gtttttcagt actaaaagct ctgataactg gaataccagt aattatttca
     3181 cggcttacta agtttaactt atcaacaagc ttttgaacca gtttaaactt aggcataacc
     3241 actgagaata acactacaat taatgataag atagcaacta ctgctactgc tataatccag
     3301 gccattgaag tatttgtgtt taaaaccttt atcactccac ctatagccat tataggtgca
     3361 tagaatacta ttctaagcaa cataaccatc aacatttgta tctgttgaat atcatttgta
     3421 ctacgagtaa ttaatgaagc tgtagaaaat tcattcattt cagtatttga aaatgacata
     3481 acttttctaa atactccact tcttaaattt ctacctacac ttgcggctac tcttgctgct
     3541 aaaaatccta ctgttattgt agctaccata ctaatcattg ctataccaag cattttagca
     3601 ccagtaaata atatataatt attttgagtt ttttctgtat caatacctat tttctcatat
     3661 tctgacctta catatgaaac agagctttgt gttatcatac tctctggcat actttcaaag
     3721 cctttgctca tctctttagt caatgtatct aactgttcct ttggcattgc tgataacaat
     3781 ttaaatatat caacatctcc actttgagta gcttgtggtg gaagtttact cattaactga
     3841 gctttcatag cttttacctc tttactatcg ccctcaagcc ctgatactat taaaatagct
     3901 ttaccaaaaa taacatttaa atcatctata gtgtctttgt ctttagtatt taactcataa
     3961 agagattcat cttttagacc tgggtactta tctttatact taacataatc ctttttattc
     4021 agcaaagtat aattatctag tactttatcc ttactacttt tatccataaa taaagtaatt
     4081 ttatttaatt cactctctct aatcacagat ggaacagagt tttctactcc accttgttgt
     4141 atacctacat ttactatatt ggatgtgtat tctggtaaag aaaggtcaca taccgcttga
     4201 attaccaata gacagactat tattactatt gataatgccg atttctttaa atatttaatt
     4261 aatttaaaca tacatttctt cccttctgta atattaatta gtctaataac attaaattga
     4321 aaaacattat tttaaacttt aaaaatattt ttcatcttca tgcttatgac atcctgcttt
     4381 taaattatct cgtacttgca ttagaagtct tctaagtact attttttctt cttcactaaa
     4441 tcctaaaaaa aactctcttt ccaattcctt gttaaattct tcaactttat tacatatttc
     4501 taaacctttg tctgtcaaaa aaatcctaga tactctttgg tccttttcat cttgttttct
     4561 ttccacaagt ccagcttttt ccatcctctt tatcataact gtagttgtag atgcttttac
     4621 tttcctttta tctgctaatt ctttttgact ttgcccatct ttcatcttta aagaaaaaag
     4681 tatttggggt tgaccatgat ataagcctaa tttatcaaaa taaaaatgag ttcttataaa
     4741 atatattcgc attaattgca agaaaacatc atttaatttt tcttcagaat tgctattcat
     4801 acttgccctc cttatttttt atattactta gtcggctaag cattatttta ctatactgca
     4861 tgcatttttt caatatattt ataaaaaatt catataaatt tcatttatta aatattttta
     4921 tatcatatag ttataatttt aataaataat agtaagattt ttaattagtc agctacttat
     4981 attttttatt gtaaagctta aaataaacct tttcatatat tttcaaataa aaaagttgtc
     5041 ccaaaataaa ttttgaaaca acatcttatt caacagtatc ttgattatat aacattatga
     5101 tactctactt atcttcatat ccatttacaa caacatctaa cttcccagtt tcaggtgata
     5161 taattagacc atgtacaata ataccatctg gaattaaagg atgccctttt attaaatcaa
     5221 tactatcttt tacagattct tctgcagaat cgaatccatg taaccattgt tttgtatcaa
     5281 ttccagcact acgaagtgta agtattacat cattagatat tcctctatct aaaattttac
     5341 caagtaactt atccgtatca acattacaca taccacatcc atgatgccct acaaccataa
     5401 cctcatctac atcaaattcg taaattgcaa ctaaaacact tctcataata cttccaaaag
     5461 gatgcattat agtagctcct gcatttttta ttagtttcac atctccattt ttcaaattca
     5521 tagccttagg caaaagctca gttaatcttg tatccataca agataagact actatttttt
     5581 tatttggatg ttttgatgtc acatattgtt catattcttt gttctctaca aatgatttat
     5641 tatattctaa tatctcttca agttttctca tatgtaatat caccctcttt aaaaattttt
     5701 ttattattat atcactatta aagtacaata tatacacata taatttaatt tatatttatt
     5761 aaattatttt ataagattat tactctacta taaatcttgt atataacttg tatttataag
     5821 tatttgtata tttatttttt ataatctaaa ctaatattat atattttcat tattttttta
     5881 attataattt ttttaaaatt ttattctaaa tgccttactt ataattaatt ttt
//
LOCUS       DACULQ010000200         5926 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.202, whole genome shotgun sequence.
ACCESSION   DACULQ010000200 DACULQ010000000
VERSION     DACULQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.202"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..576
                     /locus_tag="JA154_16345"
     CDS             <1..576
                     /locus_tag="JA154_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997400.1"
                     /translation="IQLKEISLSGIEISVSNVLSCVEEAIDISNNEDEFREYFLGNNM
                     IECIKKIKDTSYSSLEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIK
                     NPWIGIDIEDYMDKSNNNKIDDKTFNNYKASENNEIINQFKNESENIRNQEVVEFTEE
                     DEEELKQNIIENIKKNFDEKNLVQVYKYRGV"
     gene            596..1015
                     /locus_tag="JA154_16350"
     CDS             596..1015
                     /locus_tag="JA154_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997401.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEVYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            1005..1529
                     /locus_tag="JA154_16355"
     CDS             1005..1529
                     /locus_tag="JA154_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997402.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKCSQKYE
                     TDFKIKVWNFSESI"
     gene            1841..2398
                     /locus_tag="JA154_16360"
     CDS             1841..2398
                     /locus_tag="JA154_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAT7997403.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            2490..2945
                     /locus_tag="JA154_16365"
     CDS             2490..2945
                     /locus_tag="JA154_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAT7997404.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            3108..3818
                     /locus_tag="JA154_16370"
     CDS             3108..3818
                     /locus_tag="JA154_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAT7997405.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            3872..4942
                     /locus_tag="JA154_16375"
     CDS             3872..4942
                     /locus_tag="JA154_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT7997406.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            4970..>5926
                     /locus_tag="JA154_16380"
     CDS             4970..>5926
                     /locus_tag="JA154_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAT7997407.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEIS"
BASE COUNT         2558 a          507 c         1096 g         1765 t
ORIGIN      
        1 attcaactaa aagaaatttc tttaagtgga atagaaatat cagtaagtaa tgtactttct
       61 tgtgttgagg aagctattga tatatctaat aatgaagacg aatttagaga atatttctta
      121 ggaaataata tgattgaatg tataaaaaaa ataaaagata catcttatag tagtttagaa
      181 gaactttctt taaaagtaga aaataatact atttatgaca gaggagattt ttttgaattt
      241 gatatagaaa gtaatgttag agaaaaagaa tttttaaaag caataaaagt aagagtgaaa
      301 atcaaaaatc catggatagg aattgatatt gaagattata tggataaatc taataacaat
      361 aaaattgatg acaaaacatt caataattat aaagcatctg aaaataatga aattataaat
      421 caatttaaaa atgaaagtga aaatataagg aatcaagagg ttgttgaatt tacagaagaa
      481 gatgaagaag aacttaaaca aaacataatt gaaaatataa agaagaattt tgatgaaaaa
      541 aacctagtac aggtatataa gtataggggt gtataacttt gaaaaaagaa ggaatgtgac
      601 tgtaatagaa ttaatagtat ctatcagtat aatactcttg atagttacat tatcttttcc
      661 aaaagataat cttgaaaatc ataaaataaa tttatttgca cgtcaacttt gttcagatat
      721 aaggtatgtt aggcgaatta atatgtttag caactacaat acatatatat attatataaa
      781 tcaaggtaaa cacgaaggtt atgtattgag aaaagatggt aaaaacatta aatcaataat
      841 gctacctaaa aatgcaaaaa tagctaggag tattgaaata ataaagttta aatctgatgg
      901 gtcgcctcaa aaggcgtcta caatagaggt atataataat agattaagaa aaactataac
      961 aataacacct gtaagtggaa gagtacttct gaaagaggga aaatatgaaa cataaatatg
     1021 gttacttgct tttggaaagt gtcgtatcat tgtcttcaat ggtgataatt atactagttt
     1081 tatattctat atttttgtct acaataaact taaagttaaa agtagaagat aagatagaac
     1141 tacaacaaca aagtcttgaa atcataaaat ctatggaagg aattattagc aattctatgg
     1201 gaataatgaa tgtatctaat tatgaagaaa catttaaaaa gacaacatct ataaaatgta
     1261 gatatttaga tgaaaataat aatgaagaag gcataagtaa taaagaaatt atattgaatg
     1321 aaagacgaaa taagttattt gtaaattctt taaatggcga aagtagtcaa gcaggtggtt
     1381 atgaaattgg agattatgta gatgaaatgt atgtatcaat tactaataat ggtcaatacg
     1441 ttaatattaa attgaaatta tcaaaatgta gtcaaaaata tgagactgat ttcaaaataa
     1501 aagtatggaa ttttagtgaa agtatataaa aatacataaa tttaagagaa agaaatgaca
     1561 tcaataagtc atataaaata taattattga gaactatata actaaataat atcaatatgt
     1621 aaagtgtata gattaagaat atagaggata ataataaaaa tatagaataa gtaatttgcc
     1681 aaatacttgt ttatattgta taacttatga tattatgata ttagtaaaaa tttgtaatcc
     1741 tgacttgaca gaattcaagt aagagatata attttaaagg catagtaatg taaaatttac
     1801 taaaaaataa gaaatttatt ttaatatgga ggaacaaaaa atggtatcag caggtgattt
     1861 tagaaaaggt gttacatttg aaaaagatgg acaaccatgt ttagtagttg attttcaaca
     1921 cgttaagcca ggtaaaggag ctgctttcgt tagaactaaa tacaaaaact taaaaacagg
     1981 agctataaga gaagaaagtt tcaatccaag tgaaaaattc cctaaagctg ttatagatac
     2041 aagacaaatg caatatctat acaatgatgg tgagttatat tattttatgg atcaagaaaa
     2101 ttttgaacaa ataccattaa actatgagca agttgaagat gcaattaagt tcttaaaaga
     2161 aaatgaagtt gctacaataa gattttacca aggacaacca ttccaagtgg aagcgccaaa
     2221 ctttgcagag ctagaagtta cagatacaga gccaggcata aaaggtgata cagcaagtaa
     2281 tgtaactaaa gcagctacag ttgaaactgg agcagttgtt caagttccat tatttataaa
     2341 tactggagat aaagtaaaga tagatactag aacaggtgaa tatttatcaa gagtataaaa
     2401 atttatgtat ataaaagctt atatgtctta ataatataag atatatgagc ttttagttta
     2461 tatataaaaa agaaagcgag gggtgttaca tgaaacattt atatgaagaa gttgcatact
     2521 taaaaggcct agcagaagga ctagaaataa gtgctgaaag taaagaaggg aaaatgatac
     2581 ataaaatagt tgatgcatta gaagtatttg cagatgctat tgtaacattg gatgaagaac
     2641 aagaagaact tcaagatttt gttgaatcta tagatgaaga tttagcagat ttagaagaag
     2701 atattgctga catggaagaa gatctttatg aagaagatga cgatgaggat gatgaggact
     2761 tcagctatat agaaatggaa tgcccaaatt gtggagagtt agtagaaata gacgaagatt
     2821 tgttatatga tgatgaagta gatgttgttt gtcctgattg taaggctgtg atattatcat
     2881 cagaagatga ttgtgatgat gattgtacat gtggtggttg ttcaagttgt gatgatagag
     2941 agtaaaaatt cataagaatt aaaactgcct atgatgccta tgagatattt tgcttcatag
     3001 gtagttttat ttttatgagg aagggtatta tgtattttgg ttttgcaata cttggacaaa
     3061 aatagtacac tagaatttat agaataggat tttggaggga tgtaaatatg aaaataagta
     3121 aaaaattact agataatata caaagatttg aaaatgttat aaattataaa tttaaaaata
     3181 aagaatatat actagaagct cttactcata gttcatattc taatgaaaat aaaaaatata
     3241 attttaacga aagattggaa tttttagggg attcggtttt aggcatagtg ataagtgatt
     3301 atttatttaa tgaagaagct aatctgcccg aaggggaact aactaaactt agagcaaata
     3361 tagtctgtga agattcatta agtgaagtag ctaatgatat aaatttaggt attcacatgt
     3421 tattaggtag aggagaagaa gcaacaggag gaagacatag gacttcaata ttagcagacg
     3481 catttgaagc tgtaatagct gctatatatc ttgatggagg ttttgaaagt gcaagacagt
     3541 ttatacttca tcatatggaa aatataattt atgattcaag aaaaggaaat atatttagag
     3601 actacaaaac gcatcttcaa gaggtgttac aagggaatgg agaaaataat atctggtaca
     3661 ggcttataga agaaaaagga ccagaccata ataaaagatt tgtaatggaa gttggaatta
     3721 atgacgatgt acttggtata ggagaaggca agagtaaaaa agaagctgaa cagttagctg
     3781 ctaaaatagc tttaaagaaa aaattatggg aaaaataaat ataatatgta aaaatataaa
     3841 aagaaatatt tatattagaa ggatgttgta gatgaaaaag agaattatac ctatatttgt
     3901 tcctcacaaa ggatgtcctc atgattgcat attttgtaat caaaaaaaaa taacaggtgt
     3961 gagtactgat gttactagtg aagatgcaag aaatataatt gaggaatgtt tagagactat
     4021 agataaagat gcagatgtag aaatagcatt ttttggtggt agctttactg ctatagatat
     4081 tgatatacaa aaaagtttat tatcagttgc taaagaatat gtagaaaaag gtcttgtaaa
     4141 agatattaga atgtctacaa gaccagattg tattgatgaa gatatattaa gtatgttaaa
     4201 agaatataag acaagtataa ttgaactagg agtacaatcc ttagatgaaa aagtattatt
     4261 agatagtata agaggtcatc aatctgaaat tgtatacaag agttcaaaga tgataaaaaa
     4321 tagtggaatc aaacttggat tacagatgat gattggtctt ccagctgaca cagaagaaaa
     4381 gtgtatattt acagcaaaaa aatttgtaga gctaaaacca gattgtgtta gagtctatcc
     4441 aactttggta gtaaaagata caggtcttga aaagttaatg gaacaaaata aatataatcc
     4501 atttaccctt gaagagagtg taaatatagt aaaaaaagtt cttgtactgt tttatgttaa
     4561 taatatcaat gtaataagag ttgggcttca agctactgat gatatacaaa ttggaaaagc
     4621 tgttttagca ggaccatatc atcctgcatt tagagagttg gtagaagcag atatgataaa
     4681 agattattta gaatttgtta ttttgcaaaa taaaaatata aagcaaatgt tggtcaaatc
     4741 taataaaaag aatatttcta aaatcatagg aaataaaaaa acaaatgtta aatatatgga
     4801 agaaaaattt ggtgtgcttt taaaaacaca agaatcagat ttagatataa atcaattaga
     4861 aattgtgtta gatgggaaaa gtttaattag tgctaatatg agagacattc atagaaaatt
     4921 gtatgacatt tatgaccttt agttttaata atgaagggag agaaaagatt tgtatttaaa
     4981 aagactagag ttaaagggat ttaaatcctt tccagtaaaa acagatatta tttttaaaga
     5041 aggaataaca gccatagtag gaccaaatgg gagtggaaaa agtaatatat ctgatgctgt
     5101 aagatgggta ttaggagaac agagtattaa gagtcttaga ggtgataaac tcgaagatgt
     5161 aatatttgca ggtacagata caaaaaaacc tatgaattat tgtgaagtgg ctcttactat
     5221 tgataattct gaaaatcaat tggaattaga ttttacagaa gtaactataa gaagaagggc
     5281 ttatagaaat ggtgaaagtg aattttttct aaataataaa agttgtagat taaaagatat
     5341 aaaagaagtc tttttagata ctggtattgg aaaagatggt tattcaatca tagaacaagg
     5401 aaaagtagat gaaatattaa gtaataatcc tttaagcaga agaaaagttt ttgatgaagc
     5461 ttgtggcata tcaaaatata gatataaaaa acaggaagca gaaaggaatt taagtaatac
     5521 aaaggaaaat ttagaaagaa tagatgatgt atatatagaa attgaaaatc aattaaagcc
     5581 tctttttaat caacagacaa aggcaaaaaa gtacttggaa ataagtgaaa aattaaagac
     5641 acttgaagta aatagcttta taagagaaat tgaaggaata gaaaaagaat taagtgaggt
     5701 taatgaacat cgtaaggtta ttgaaaagga gctcaatgaa aaagaagaac aaaaaaatgt
     5761 tgtagagaaa aagcaagaag atattaataa agaggtagag gtattacaag atgtaattga
     5821 aaaatctgta gattatataa attcaattaa aggagttata tcaaaaaaag agtcccaaat
     5881 aaatttaatt aaggaaagaa taagaaattt tacgaatgaa attagt
//
LOCUS       DACULQ010000201         5901 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.203, whole genome shotgun sequence.
ACCESSION   DACULQ010000201 DACULQ010000000
VERSION     DACULQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.203"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            120..686
                     /locus_tag="JA154_16385"
     CDS             120..686
                     /locus_tag="JA154_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HAT7997408.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            726..1307
                     /locus_tag="JA154_16390"
     CDS             726..1307
                     /locus_tag="JA154_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemical-damaging agent resistance protein C"
                     /protein_id="HAT7997409.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1364..2281)
                     /locus_tag="JA154_16395"
     CDS             complement(1364..2281)
                     /locus_tag="JA154_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT7997410.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2411..4042
                     /locus_tag="JA154_16400"
     CDS             2411..4042
                     /locus_tag="JA154_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HAT7997411.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFVRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4045..4992
                     /locus_tag="JA154_16405"
     CDS             4045..4992
                     /locus_tag="JA154_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAT7997412.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5277..5540)
                     /locus_tag="JA154_16410"
     CDS             complement(5277..5540)
                     /locus_tag="JA154_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HAT7997413.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5639..5884)
                     /locus_tag="JA154_16415"
     CDS             complement(5639..5884)
                     /locus_tag="JA154_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997414.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVINKVKTG"
BASE COUNT         2128 a          807 c          980 g         1986 t
ORIGIN      
        1 tataataaat aatttataaa tatagccaac ttagcctaaa tacgtatata atatagctaa
       61 tatggttctt atacttgata ccatcaataa aactaaaaat ataaaaagga gaacttaaaa
      121 tgagtgttag tttacaaaaa ggacaaagag ttagcttagt aaaagataat aatcctgtaa
      181 aaaatctagt agtaggctta ggatgggata tgaataaatt gggtaaaaaa aattatgacc
      241 tagatgcatt tgcaatagca ttgactaacc aagataaaat gaatgttaga ggtgacttgg
      301 tttattttgg aaatcttaaa cacccttcta aagctattat acacactgga gataacttaa
      361 ctggaaaagg tgaaggtgat gatgaacaaa taattgttaa tttagaagac atacctgaat
      421 atgtacataa aatagttttt ggagtttcta tatacaaagc taaaaaaaga gaccaggatt
      481 ttggtcaaat gaacaactct tttataagat taattgattc aaattccaag caagaattat
      541 ttaaatataa tcttcaagaa gacttttctg gtaaagtaac agttttggca ggagaaattt
      601 atcgtagaaa tgaagaatgg aaatttaatg ctttaggaat tggtcaaaat gaagagcttc
      661 gtgaattaat taatacatat aagtaataat tacaataaaa aatttataat gaggagttgg
      721 tttaaatggg tataacttta gcaaaaggac aaaaagtaag tttaacaaaa agtaaccctg
      781 gtctaaaaaa agtaatagta ggactaggat gggatattaa taaatatgat ggtggctttg
      841 atttcgattt agatgcctct gcatttttaa ctggaactga tggaaaagta acgaatgatg
      901 gagactttat attctataat aatttaaaac acgcttctgg agctgttgaa tatatgggtg
      961 ataatagaac aggtgttggt gatggtgatg atgaacaaat aaatgtagac ttatctaaaa
     1021 tacctcaaaa tattgctaaa atatcattta gtgttactat aaacgaagca attacaagaa
     1081 gacaaaattt cggacaagtt gaaaattcat atataagaat atataatgaa gaaactaatg
     1141 aggaacttat taaatatgaa ttgggtgaag attttagcat agaaacagca atagttgtgg
     1201 cagaactata tagacataat ggagaatgga aatttaatgc tttaggttct ggatttgagg
     1261 atggacttgc aggattgtgt aaaaattttg gcgttaatat aggctaatat atctaaataa
     1321 aaataagagt atattgatat actcttattt ttatttagat atattataaa ctgtttacac
     1381 ttctactctt ctttctcgct tgttttttcc catattctac tgaaatatca aaaaatgctg
     1441 aaatattttc tgtaaaaaac ttgcttctat gatatgcaat atcaaatgtc ctatgtaaat
     1501 taataccttc aatatcacaa gcccacaaaa gatgcttttt taaataatcc tcaataagta
     1561 attcggatat aactgcaatg ccaaaattat ctacaactgc tcttaatata gcttcagagt
     1621 tataacatga ccatctaata ttcattggga tttttaattc ttttaattgc ttttctaatt
     1681 gtgctcttgt accacttcct aattcacgca taattaatgg ttgatttgaa agttctgaaa
     1741 cttttataga gtctctttta taaaactcat gcttgtgtga acatataagt acaagattat
     1801 cattaataat gctttttgta acaatatctg ggtgcttaat ttttccttct acaatcgcaa
     1861 tatctaattc actattaagt aatttttttg caatcagtct tgtatcttct acaaatactt
     1921 caggttctat aagaggattt accttgtata attctaacat tattggactt attacacatg
     1981 taccaacagt tactgttgcc ccaactctta tacggatatt ttttgcacaa tgcttcatac
     2041 aatcattcat ttcttcattt acagctaaaa gatgcctccc atatccaaga agttttttcc
     2101 ctgcctcagt taaatataat ttttttgaaa ttctatcaaa tagcttaaca ccatactctt
     2161 tttctatttc tgaaatagct tggctaactg atgactgggt aatatacata tttcttgcta
     2221 cttcactcat tttaccacat tcagcaacct taacaaagat ttcaagacta cgaattgtca
     2281 tattatcaca accttagcaa acattagtaa atgcttatga ttttattaat attttagtat
     2341 tttacaaata tattgtcaag aagtataatt ttattataga gttataaagt agattggagt
     2401 gtgatatatt gtgaaagtat taattatagg tggagttgct gctggaacaa aaactgctgc
     2461 aaaaataaaa cgtgaaatgg gtgataattg ctctgttaca atactaaata aaggtgatga
     2521 tatttcttat gcaggttgtg gtcttccata ttacgttggt aagataattg aagataaatc
     2581 atctttaatc gtaaatacac ctgaaagttt ttctaaacta acaggagcag aagttcgttg
     2641 tggagttgaa gttacatcta ttgatagaaa tgcaaaacaa gtaactttga aaacgctttc
     2701 aacaggagaa aaagaaaatc taagctatga caaacttgta attgctacag gggcagaccc
     2761 aataaaacca ccaatacctg gaatagattt acctggagta ttctttgtgc gtactccaaa
     2821 tgatgctata aaactaagag attctattga aaacggtatt aagagagctg ttgttattgg
     2881 tggaggtttc attggtcttg aaattgctga aaatctttct gcaatgggta tacgtgtaag
     2941 tgttgttgat atggctgaac atgttatgcc tggatttgat actgattttg ctgaatacgt
     3001 tgaaaatcat atggctgata aaggcataat gatttttaca ggtgaccagg ttgtaggtat
     3061 cgaaggtgaa aataaagttg aaaaacttcg tactaaaaat agagctatta aaacagattt
     3121 agtggttatg tcagttggaa tccgtcctaa cacaggtttt ctaaaagaca caggtttaga
     3181 atttgcccct aataatacaa ttattgcaga tgaatatatg cttacaaatg acaaagatat
     3241 atacgtagtt ggagactgtg cttttgtaaa aaattcttta acaaataaac ctacatggtc
     3301 acctatgggg tcatctgcaa atcatgaggg tagaatttgc gcacaaaata tatctggaaa
     3361 atctaaaact tacaatggtg ttcttggaac aacagtagta aaattacctg agctcaatgc
     3421 aggaaaaaca ggtctaagta aagaaatagc tgaaaaagaa ggttataatg tttgctctgt
     3481 aaccatagct actgatgata aagctcatta ttatccaggt gcatctaatt ttattataag
     3541 aatggttgct gaaaaaaata ctagaaaatt attaggtgtt caagttatgg gacctggagc
     3601 ggttgataaa atagtagata tcgctgctac tgcaataact ttaggcgctg acctttattc
     3661 attagaaagt atggaccttg catatgcacc acctttttca acagctattc acccttttgt
     3721 agtggcagta aatgttttac aaaataaact taatggagaa cttgatagtg ttttacttga
     3781 cgaaattgag gattttgaca gttggactaa acttgatgtt tcaaaagctc cttctattcc
     3841 tagtttacgt tatattcctg ttggtgaaat taatggagaa attgaaggcc ttgctaagga
     3901 tgaaaaaatt cttcttatat gtgctaaagg taaaaatagc tatatggcgc aaaatcgctt
     3961 aagacgcttt ggctatacaa atacgaaagt tcttgaaggt ggaatactat tttatccaaa
     4021 tcttaaggta atggaggagt aaatatgagt aatgttacag cagaacaaaa aaaggaagta
     4081 aaaggtagag gttttctacc aagcagggat ggagaacatt ttgcagcacg tataataact
     4141 gtaaatggtg taataaatgc atcacaagca aaaaaaattg cagaagctgc tgaaaaattc
     4201 ggaaacggtc agttagcatt tactactaga ctaacagtag aattacctgg tgtaaaattt
     4261 gaagatattg aagctctaag tgaatttatt gctagcgaaa atctaataac aggtggaact
     4321 ggatcaaggg ttcgtcctgt tgtggcttgt aaaggtactg tttgtgttca tggtctagct
     4381 gatacacagg cacttgctgc tgaaatacac gaagaatttt acaaaaattg gtatgatgta
     4441 aaactacctc ataagttcaa aattggtgtt ggtggatgcc ctaataactg tattaaacct
     4501 gacctaaacg atcttggaat tgttggtcaa cgtgtacctg actatgattc agagctttgt
     4561 gttggttgta aaaaatgtgc tgttgtagag gtctgtcctg ttaaagcagc taaattaaca
     4621 gataaaggta aacttgaaat agatagtaat ctatgtaata actgtggtaa gtgtatagaa
     4681 agttgtaatt ttgatagtat tgaagaaaaa gaatctggtt ataaagtcta tataggaggg
     4741 aaatggggaa aatcagttcg tcctggaaca cagattgata gattattttc aaaagaggaa
     4801 cttatgactt taattgaaaa agcaattctt ttatttagag aacagggtaa cacaggtgaa
     4861 cgttttggaa taactatcga tagaatcggt gctgataaat ttatagaaat gcttttatca
     4921 gatgaagttt tggaaagaaa agaagagata ctagaggctc ttttacacct tacaggtgga
     4981 gctgtatgct aacaataaat attttaattg tacagataac ctatggaaaa aattttagtt
     5041 aagaataatt aaaattctat atatgatata tgataaatga ggctatctca aaacacagat
     5101 acgccttttt ttcattgttg aaatgataaa gtatatttga gttcatataa aaaagagtgt
     5161 ctcgtgaatt ataaaattca ttttgaggca cccacattat tttataataa ttgcttatta
     5221 attttatttt aactatataa aaagtacaaa aagtaagaat atccaatatt ctaaaattaa
     5281 ttttgaattg tattgattga aaagtttact attccactat ttccatcttg atttactact
     5341 agataccatg ttccagtatt aggaactctc attctacatg gatttttctt tgcaaatcca
     5401 ccatagtaat cataatttct ttgattttta aatttcacat aatttatatg gtctaacaat
     5461 attacattgg cttgtttctc taaatttacc tctacaatat cccctttttt taagttcatc
     5521 ttactataac tatattccat tgaattactc ctccagtatc gaataatctt tgttatttac
     5581 tcatctcaaa tatgtaaaaa ttcatttaaa atattacaaa tactatataa ttagactatt
     5641 atccagtttt aaccttattt attacaatcc aaaggttatt aaaaatatta ttggcaacaa
     5701 cttctccatc ccatacaact ctttcttctt tattttctaa taaagcatag tacccagagt
     5761 cagaactttc atctttatag actgtaacat aaaactcttc atcactatat tcaggtatat
     5821 ttttaaagtc ttcagatatt ttacatgtta atatataacc ttctaattct tcatctataa
     5881 acaaagcaat tcctccaatc t
//
LOCUS       DACULQ010000202         5900 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.204, whole genome shotgun sequence.
ACCESSION   DACULQ010000202 DACULQ010000000
VERSION     DACULQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.204"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            437..1708
                     /locus_tag="JA154_16420"
     CDS             437..1708
                     /locus_tag="JA154_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAT7997415.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKNFNLDEVVKLDDERRKTLQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            2076..3581
                     /locus_tag="JA154_16425"
     CDS             2076..3581
                     /locus_tag="JA154_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT7997416.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3695..4393
                     /locus_tag="JA154_16430"
     CDS             3695..4393
                     /locus_tag="JA154_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HAT7997417.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4696..5766
                     /locus_tag="JA154_16435"
     CDS             4696..5766
                     /locus_tag="JA154_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997418.1"
                     /translation="MKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGLIGALIS
                     MVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILIVMISGA
                     GSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAVGLISLF
                     KHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIESKKDAF
                     WGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSVALILGI
                     YTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPYTGYMGL
                     LILVCMVYKAITCRKKSTQSQE"
BASE COUNT         2413 a          580 c          962 g         1945 t
ORIGIN      
        1 agtatttttt gtaagcagtt ctaaaagatt caatttcata cctattactt gaatcattca
       61 tcaaaataat tctaatttaa aaaataattc tatccaaata atagatatat aggactatgt
      121 tataatataa aaaaatttat ataaaagtta ataactatac aagattgata aagaaaaagt
      181 caaaatagat aattgattag caaaatagat ataattataa tgaagttaaa tctagcaata
      241 ttctcctaaa attaaatttc atttatttaa ttaaatataa tttgacaatg ggcaaaaaag
      301 tgagtaaaat ataaataaaa taaaaataac aggttcggat aaagaaaaag gtttcttaac
      361 ctcaagcata aagtatatta tctaagttat ttgagattaa gagataaatc caatcttagg
      421 tactgaggag gaaaaaatgc ttgatataaa aagaataaga gaaaatttag attgtataaa
      481 aaaagcaatg gaaagaaggg gcgaaaaaaa ctttaacctt gatgaagtag ttaagttaga
      541 tgatgaaaga agaaagactc ttcaagaagt tgaagttatg aaaaatgaat taaatacagc
      601 atctaaaaat ataccaaatc ttataaaaga aggtaaagat gtagaaaatg aaaagattaa
      661 attaaaagag ttatctgata aaattaaggt aatagaccaa aatcttaaag aagtagaaga
      721 taagatggaa tacctattaa tgagaatacc aaatgtacca catccagaag taccacaagg
      781 tgaaactgat gaagataatg ttgaagtaag aacttgggga aagactacta cctttgattt
      841 tgaatctaag gctcattggg aaattggaac agaattaggt atacttgatt ttgaaacagc
      901 tagtaaaata actggttcaa gatttactct ttacaaagga ttaggtgcta gattagagag
      961 agcattatta aatttttact tagacacgaa tacaaaagta aatggatata cagaagttat
     1021 tccaccattt atggcaaata gaaatagttt tttaggtaca ggacagttac ctaagtttga
     1081 agaagatatg tttaaaatag aaggtatgga ctattttatg attccaacat cagaagttcc
     1141 tttaactaat atacatgcta atgaaatact taaatttgaa caattaccga taaattatac
     1201 agcttatact ccatgtttta ggtcagaagc tggttctgct ggaagagata caagaggttt
     1261 agtaagacag catcagttta acaaagtaga aatggttaaa atagttgcac cagaagaatc
     1321 ttacaatgag ttagaaaagc ttactaacaa tgcggaaact atgctacaat tattgaatct
     1381 gccttataga gtagtaaaga tatgtacagg tgatttagga tttacggctt cttttaaata
     1441 tgatgtagaa gtttggatgc caagttacaa taggtatgtt gagatttcat cttgttcaaa
     1501 ctgcgaagat ttccaagcta gaagagcagg aataagattt aaaagagata aagattctaa
     1561 agctgaatat gtacacacat taaatggctc tgggttagct attggaagaa gtgttgcagc
     1621 tatattagaa aattatcaac aagaagatgg ttctgtggtt gttcctgaag ttttaagacc
     1681 atatatggga gtttctgtta ttaaataaga gttaaattat gtatttttaa gtaatacaat
     1741 aatatagagg taataaaaaa taattaatat aaaagtgata caataatata gaagtaataa
     1801 aaaaatagtt aatataaaag tgatacaata atatagaagt aataaaaaat agttaatgta
     1861 aaagtgatac aataatatag aagtaataaa aaaatagtta atataaaagc gatacaataa
     1921 taatagaagt aataaaaaaa aataattaat ataaaaatgt gtacaaacta atgaatattg
     1981 atatagtaag ctatcatatg aaagagtatg tacacatttt ttttactaat ttttcacaat
     2041 atgaacataa ataatttaca ggaaaggaat cttttatgaa aattcaaaat actgagtggg
     2101 gatatattga atggaaacat acttatgatg aaaataatcc aaaacaagct atgaatatat
     2161 acattgcagt aactatgcct ggtaagaaac attttaatca tgttcattat ggtcaagagc
     2221 agatgatata tattttagag ggagagggtt tgtatattat aaatggagtt tggaagccat
     2281 tttatcaagg tatgattttt tatatagaat ctggtagcac acatgaaact ataaatacag
     2341 gcgatagaga aataaaagaa cttattgttt caaataatgt agatgatgta ggagagagtg
     2401 aagttataga tattaatcct aataattatt taaagaagac cttgataaat tatagtgaaa
     2461 gtacattaaa tttgtatgca gcagtagaat caattcgtgg tcaatttata gacccgttta
     2521 aaattccatt aattatatat gatgattcat ggaatatagt tttaaaaaat ccttattttc
     2581 cacttttttg ttttgaaaaa tgtaatccta tgaaatttcc tcaaaattgt gattgtatga
     2641 atcaaaaaag tagcaatcaa tttgtatgtg aatatgggat aactatatat aatataccaa
     2701 tactgtacaa aagcaatagc ataggtgtta taagaggagg gtatgtacta ttatctgatt
     2761 tgaatttaga tacagagcat aataatttat atgatattcc agagggagct gctagaagta
     2821 taaaacgcct cttgaaacaa atatctaaaa atataataaa tttttgctct tttaatgata
     2881 taagaaagga tttacaagaa aaagagaaga ctatagcaag aacatatcat tatggagagc
     2941 aacttgaaat gaatttaaaa gtagctcaag atatggttac taatttaaga ataaatcatc
     3001 actttttatt taatacatta aattcaatgg caagcatagc actagatgat ggtagttatg
     3061 atttgtatag tgcaataata gatttatcaa gaatgtttag atatacaatg aggtctgatt
     3121 taagatttgt agaattagaa tctgaaatat tatacattaa aaattatttg aatttacaga
     3181 aattacgtta tggagatgca cttaaagtaa aatatcttat tccagagaag ttatataacc
     3241 tttctgtgcc ttttaacttt attcaaccta ttgtagagaa tgcatttaca catggattta
     3301 gagatattga cacagaaaag cgaattgaaa taattgcaag attagattct cagtatgcca
     3361 ttatagagat tcataataat ggaactatct tagatggaaa taatatagac aaaataaagg
     3421 caggtattcg tagtaataat ggtcatggtt tgtctttaat atatacaaaa ttcacatctg
     3481 cttatggaaa taattttgat atggatataa agtcgagtga taatgaagga acttacataa
     3541 tgattaaaat tcctattgaa aattataagg agtatgtata gaacttgtga aaaattaata
     3601 agttttataa atgttaagaa atatattaga tgtttttatg ttgttttata tatcttactt
     3661 ttttatatta ttaagctaaa agggaggtat tctcatgaga agagcactaa taatagatga
     3721 tgaagaagct gttctaaaaa taataacaca ttttatagaa atgaaagata tgccaataaa
     3781 aattgttggt aaagcaacat ctggtgatga agcagtagat aaaatcattg gtttagagcc
     3841 agacatagtt tttatagata ttcaaatgcc aatatacaac gggttagaag taatagaaaa
     3901 aacatctcat ttatctaaaa aaataaactt tgtagtaata actgcattta actattttga
     3961 atatgctcaa aaagctttga gattaaatgt aaaagatata ctccttaaac cattggatat
     4021 gaaagaattt actaagtctg tagaaaaaat tattggatat caatatacca ataatgattt
     4081 attaaatgag atactggaat atataaatct aaactatagc aatgacttaa agttaaatga
     4141 ttgtgctaga ttatttttaa ccaatccatc taatatatct aggattttta aaagtaattt
     4201 aaatacaact tttatatcat atctaaatca tataagaata gaaaaatcta ttgagttact
     4261 tgaaaatact acaaaaagta taaatgagat tgcagaaata gttggatata atagcctaaa
     4321 taacttttat aaaaacttta agatagaaaa aggaatgact ccaaaagttt ataaactgaa
     4381 cactaaaaat tgaaattgat aaataaaaag tcaatataga taattgtttg agtgtgtctg
     4441 aataagtata ctttaattat gataaaaatt aaagcgcttt aaacatattt aataatatta
     4501 aaccttataa aagctgtacc tatttatata aaaaaatata tataaataaa aagaaaaaga
     4561 tatacaaaat aaaaaaagtg aattaaatac tgtttcaaaa agacagaatt ttcatttaat
     4621 ttactgagag tttatatttc ttaaaggaag gtaactttat aatggaggta atttaatatg
     4681 tcagacaaag tgaatatgaa aaatattatc tgtattgcag gcgcttatct agcaacagca
     4741 attggttctg ggtttgctac tggtcaagaa attctacaat tttttgcctc acaagggtat
     4801 atgggtttaa ttggagcatt aatatcaatg gtattgtttt catttcttgg tgctgaagtt
     4861 ataactagag gtagagaatt aaaacttaaa gaaccaacaa gaatttacac tttttattgt
     4921 gggaagattt taggaaaatt ttacgaatgg tttagtccaa tatttttatt tgggattctt
     4981 atagtaatga tttcaggtgc aggctcaact ttaactgaat actatggtat aaatccttac
     5041 ttaggaagag tattgatggc tgttgctact ttaattacag tgtctatggg tttagaaaaa
     5101 ttagtaagca tacttggacg tattggacca gtgatagcag tatttacaat tgctgttgga
     5161 ttaataagct tgtttaagca ttttgacggt ttatctatgg ttggagaagc tatgaaaaat
     5221 attgaagtta caaaagcatc agaaagtcca atcgtttctg gtgtaatata taatacatta
     5281 aatattattg taatgattgt ttttttaaca ggaatgggag cttctattga aagtaaaaag
     5341 gatgcttttt ggggaggttt attaggtgga atagtgttta tgactgctgg tatagttatg
     5401 tatttggcta tgatttcaga tataggaaat ttgtatatga aagaaatacc atcactttat
     5461 ttagcagata atatatctcc gattgtaggt gtatgttttt cagttgcatt aatattaggt
     5521 atctatacaa cagctgttcc acttttatgg tctgtaacca atagaattgt tgaagatgaa
     5581 catcctaaat ttaaattggt aacaacagta atagctattt tggcttgtat aggaggtttt
     5641 ttaccgtttg gtaaattagt aaacatttta tatccatata caggttatat ggggctttta
     5701 attttagtct gtatggtgta taaagcaatt acatgtagaa aaaaatcaac acaaagtcag
     5761 gaatagagcc acttattaat agtttttatt taaaaaaatt ttttatataa tccgaattta
     5821 aaaagtcaaa atagttaaat aaattgcaaa acatgataat atatttaatt aaaataatat
     5881 agtaaaatat aaatataaac
//
LOCUS       DACULQ010000203         5897 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.205, whole genome shotgun sequence.
ACCESSION   DACULQ010000203 DACULQ010000000
VERSION     DACULQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.205"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1326
                     /locus_tag="JA154_16440"
     CDS             <1..1326
                     /locus_tag="JA154_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HAT7997419.1"
                     /translation="ILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFEL
                     DVTNLLKKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETF
                     ARVSADNFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRAS
                     LAKSSIKNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDK
                     FCGSEALVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISP
                     LPISVNFSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNL
                     QDIGFIVAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVYDKDVDKSQIVIKSIVSLIK
                     QLGIRVVAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1359..4796
                     /locus_tag="JA154_16445"
     CDS             1359..4796
                     /locus_tag="JA154_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT7997420.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLITYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVELGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            4814..5560
                     /locus_tag="JA154_16450"
     CDS             4814..5560
                     /locus_tag="JA154_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAT7997421.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5509..5817)
                     /locus_tag="JA154_16455"
     CDS             complement(5509..5817)
                     /locus_tag="JA154_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997422.1"
                     /translation="MNFLKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIEKYKT
                     SYKQLLVNPNIEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         2456 a          586 c          896 g         1959 t
ORIGIN      
        1 atactttcat ttttattttt aggtattata atttacatat tatattcaaa agcaaatagt
       61 caaaaaacta tagaaaaagt agcatatacg gattttgtaa caggttattc caactggaga
      121 aagtttgaat tagatgttac aaatttatta aagaaaacat cacaaaataa taaatatgca
      181 atggtcatat tcgatattga taaatttaaa gcaattaatg atatatatgg ccataaaaag
      241 ggaaatttaa tattaaaaga tattgcagat actttaaatg aattgactga tatcaatgaa
      301 acttttgctc gtgtaagtgc tgataatttc aatatattat taacatataa caaaaaagag
      361 gatataataa atattatcaa aaagattatg gcaaataatg aacttgtaaa cttatctttt
      421 ggaatttatg aaatcaaaga taaggattta tctgtaagtg tatatagtga tagagcttca
      481 cttgcaaaat catcaataaa aaataatagt gatgtaaatt ttgcattttt taatgacaaa
      541 ttaagagaga aattattatt tgaagataag atagaaaaag aaatggaata tgctcttgaa
      601 agtgggcaat tcgtaatgta tttacaacca aaatacaata ttaaattaga taaattttgt
      661 ggaagtgaag ccctagtcag atggcaatat acagaaaaag aggtcatata tccaggtgat
      721 tttattccta tatttgaaaa aaatggattt attagaaaga ttgatatgta catattagaa
      781 caagcttgta aagaaatccg gtctttattt gataaaggaa tttcaccttt acctatatct
      841 gtaaacttct caagagttga tttttttaaa aaagatttta ttgaaaacat agtaaatata
      901 tgtgatagat ataaaatacc atatagttta atagaaattg aaattactga atctagtatg
      961 tttggagata ctgatactct atttaatgtt agtagaaacc tacaggatat tggctttata
     1021 gttgcaatgg atgattttgg ctctggatat tcttctgtaa atatgcttaa aaatatacca
     1081 ttaaatgtaa taaaattaga tagaggattt tttgtttatg ataaagatgt tgataagagt
     1141 caaattgtta taaaaagcat agtttctctc attaagcaat taggaataag ggttgttgct
     1201 gaaggaattg aaacaagaag tcaaatagaa atgttaaaaa aagcaaattg tgatattgtt
     1261 caaggatatt atttttcaaa accattacca attaaagaat ttgaaaaatt agtctataaa
     1321 atatagtcag cgagtcttaa atgggaggtg gtgattaagt gataagagca aaatcgttaa
     1381 aaaatgtact gagtattgtt ttaatatttt tatttacaat aatttctttt tctgattttg
     1441 catttaaaac taatgtccag gaagaagaaa atagtaaaat taatttaaga gaaatatccc
     1501 tacaaagttc caatgctata aaaataaaat tagatagttc tctatccttt attaaaagtg
     1561 tggcaaatat ggtttcttat tttaatacta tagataactc aaaaatagtt ccattattag
     1621 ctaatatatc aaaagaatct gactttcaaa gaatctctat tgtatctcct gatggaataa
     1681 gtcataccag tgatggcaaa attatcaatg tgtcaaagta tcagtatttt aagaaagcta
     1741 ttaaaggaga aagtaatata tctgaagttg tgaaatctcc aattgatgga aaagatactt
     1801 ttgttatagc tactcctgta tttaataaga ataacaaaat aattggtgtt ttatacggtt
     1861 cttactacaa tgataaatta gctgaattta tagatataac aagttttaat agtgaagggt
     1921 acatagatat atttgaatct agtggtcaat ttgttttaaa atcttcacat acaaacaaac
     1981 tgattaagtc aagtagtaac atatatgata tatttaaaaa agcttctttt gatgatggtt
     2041 atagctataa aaaaatatat gaagatacaa aaaataacaa gagtggcttt acctcatatg
     2101 aatacaaagg agaaaatcgt tatgctactt atacacccat tggtgtaaat gactggtatg
     2161 taatttctgt gataccacaa tatactgtaa gaagaaacat tgataaaata aatcaattag
     2221 cattttctct tattttcaag gtattcttat tgtttattat gcttataaca tatattattt
     2281 ataaaaacaa taaatctcag aaagatatac taaaagctaa caaagaaatc tcatctatta
     2341 caaatagcat tcctggagga gtacaaaagt gtaccctggg tgaaacatgc aatattaatt
     2401 ttttaagtga tggatttata gagttgtttg gttatacaag agaagaaata aaaacattgt
     2461 ttaataatga tttttatagt ttaatacata aagatgatgt tgaaagaata aaaaaagaaa
     2521 tttttaaaca actttacaaa aataaaactg ttgaattaga atataggatt ataacaaaaa
     2581 atggtaatac agtatgggtt ttagataaag gggagcttat aacagaacct gatggaaaag
     2641 aatatttttg ttgtgtaact gtagatatta ctagaacaaa acaattagaa caagagatgg
     2701 aacaaaaaaa tctagaactt gaaatgatat cagaaaatgt tttaggtggt ataattataa
     2761 cagaatttaa tgatgatttt actataaaat acgctaactc tggttatctt tcaatgattg
     2821 gatatacacg tgaacaatta gaaaatgaac ttaaatacaa agcttgtaat cttgtttttt
     2881 atgaagatag aaagaaggct cttgaaagta tacaacatca attaaacaca aaaggaatag
     2941 ctgtatatca acatagaata agaagaagag atggtaatat tatttgggtt tctataaaag
     3001 gaaggcttat tgttgatgat gatggtattg aaaaaggtat ttggattcta aatgatataa
     3061 cagatttaaa aaatgcagaa caaaaactta aaataaatga agaaagattt agaattgcat
     3121 taaagcaaac atcaaacact atatttgagt atgatataaa aacaaagtca cttttaaata
     3181 ctagtaaaga gcttgataaa tataatattt ataaaattac taaaaatgtt ccatattctc
     3241 tagtagaatt aggagttatt cataaagatt ttagtgatga ttttataaat atgtatgaaa
     3301 atgtaataaa tggagcaaaa aaagcaagtt gtgtagttaa gatgaaactt ccagatgaaa
     3361 attatatatg gaataaagtt accttgacta cgatttttga tgaagatggt aatcctgtaa
     3421 gagcaattgg tatacaggaa gatataagca aacaaaagga agcagagata agatacaatc
     3481 aagaaggtaa gtatcattca gctatattgt cggaagcaat gataacttat gaaataaatg
     3541 tatcaaagga tacttttatc aaaggaaatg aaagttggca aaaaccattt ggaattaagc
     3601 ctgacaataa ctattctcaa atggtaaaaa ctatagcaaa tagtattgta cataaagaca
     3661 ataaagaaac atttttaaat acatttttat gtgaaaactt attggatgca tataaaaata
     3721 atgtgactga aataaaatta gaatatcgaa gaatgaatca agaaaataaa atgatttggg
     3781 tcatttgtac aatgcactta ataaaagatt ctattacgaa cgatataaaa gcacttgctt
     3841 atgttaaaga tattgacaag caaaaaaatg aagaaattag attaaaatta aaagctgaaa
     3901 gagatactct tacaggatta tataataaag gtactacaga aaaattgatt aatgatttct
     3961 tgaactctga ggaagcaagc ttaggaatgc atgcattttt aattgttgat atagataatt
     4021 ttaaatctgt gaatgataat ttaggacatg tatttggaga taaagtatta tttgaagttt
     4081 ctaaaaacct aaaacctata tttagaaaag atgatatagt aggtagaatt ggtggagatg
     4141 aatttatagt attcttaaaa aatattcagt cttttgagaa tatcaaaaag aaagcagaaa
     4201 aaatatgcga tatatttaga aattcttata cagaaaataa aaagacatat aaaatatctg
     4261 gaacagttgg aatttcattt gtaccagaac atggaactgt atttgaagaa ctttataaaa
     4321 aagcagacat agcactttat tatgcaaaga gcaaaggaaa agataagtat gcactttata
     4381 atgattgtat ctaccaaaat gaacttgaaa ataatataag tgataataat ataaagttag
     4441 cagataaatg tgagaaatcc aaaccagtac ttaaagaaat tcttgattct attgataatt
     4501 atatctatat agtgaatcca aatacttatg aacttctttt tgtcaataat aaggtgtctc
     4561 gtttggattt agagataaaa ttaggagaaa cctgttataa atcactatta aaatctgata
     4621 gaccatgtaa taattgccct cttaacggat tagaagaaaa taagcattca aaattcaaaa
     4681 tagagacaaa tacatttggt aaaaatatca gtatagcaag ttggattaat tgcatagatg
     4741 gagaatacaa ctgtcttata gagtgtatag attcttcttt atacaaaaat aaataaaaaa
     4801 ggactatgaa gatatgttaa atagtataac tttgatactt ggtataatat ttacaattta
     4861 ctatttttat ctcaaaatag tttttggagg aatttcattt agtgagattt tcttagttgt
     4921 aggaattgta ctaattatct atcaaatatt taagaaaaaa attaaagaaa agaaggtttt
     4981 ttatagggtg ttaaaaataa taatcagcat ttttttaata gtatttgtag ttactgagag
     5041 tctaattatt ttttatccca aaaatagttt agaatcaaaa agtgattatc tcttaatttt
     5101 aggagcttct gtaaaaaaga ctacaccaag tacaaccctg aagggtaggt tagacactgc
     5161 attgaaatac ctaaaaatta atgataattg ttatgtggta gtatctggtg gtaaaggaag
     5221 tggagaaaaa ataactgagg caaaagctat gaaagattat cttataaaaa atggtataga
     5281 taaaaatagg ataatagaag aggataaatc cacgaatact tatgagaatt ttaaatattc
     5341 aaaattgaag atagaagaac atagtcaaag aaaactaagc gatttaaaag taaaaatagt
     5401 aacaacagat tttcatgttc tcagaagtaa aatattagca tatagaaatg gatataaaaa
     5461 tacgtctttt tatgctagta aatcaaaact ttcatttgta ccaacttact atacaagaga
     5521 attttttgca ttatggaaaa caatcatttt tgatagataa taaataaaaa gactagatat
     5581 aaactcatat ctagtctttt ctattccctt aattctaatc cattttccaa ctttacttgt
     5641 tgtagaaatt tcaatattgg gatttacaag taattgctta taagaagttt tatatttttc
     5701 gattccaaaa tataattttc cttcataatc cattataaat ccaaatggtc ttactcttgg
     5761 tttattatct tcaactgtag caaaatagaa tgttgagttt tcttttaaaa aattcattat
     5821 tttttcatga ttaaatcctc tttgtatctt atactaatat aatgaatata aataaaatat
     5881 tcattatact taatata
//
LOCUS       DACULQ010000204         5877 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.206, whole genome shotgun sequence.
ACCESSION   DACULQ010000204 DACULQ010000000
VERSION     DACULQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.206"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(5..205)
                     /locus_tag="JA154_16460"
     CDS             complement(5..205)
                     /locus_tag="JA154_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAT7997423.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            672..2162
                     /locus_tag="JA154_16465"
     CDS             672..2162
                     /locus_tag="JA154_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HAT7997424.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            2442..3938
                     /locus_tag="JA154_16470"
     CDS             2442..3938
                     /locus_tag="JA154_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HAT7997425.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            4072..4986
                     /locus_tag="JA154_16475"
     CDS             4072..4986
                     /locus_tag="JA154_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT7997426.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTNESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGVLKIL
                     ERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            5104..5295
                     /locus_tag="JA154_16480"
     CDS             5104..5295
                     /locus_tag="JA154_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997427.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            5518..5868
                     /locus_tag="JA154_16485"
     CDS             5518..5868
                     /locus_tag="JA154_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997428.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         2357 a          594 c         1066 g         1860 t
ORIGIN      
        1 ataattatat tctatttacg ttttcagctt gaggtcctct attaccttga gttatatcaa
       61 agctaacctt ttcgccttct tctaaagttt tgtatccatc attttgtata gctgagaaat
      121 gtacgaaaac atcatcttct ccctctacag atatgaatcc aaatcctttt tcattgttaa
      181 accattttac tattccgttt ttcattataa gaaaacctcc gcattattaa aaaatacaaa
      241 ctaaaaaaat aagtctgttc tattaatata acataaattt taaataaaag caaataaaat
      301 ttttaaaaaa attaattact tttagaattt aataaaaatt gttaaattat acagtgagaa
      361 gtattgtaaa atttgacgaa aatgtaaatg tagataataa atttaatatc aaaaaatgta
      421 acaaaaaggt atgtaagtaa aaagtaaaat taaaatatat aataaaaaat attatatatt
      481 agattgaaaa gtaaaaataa aaatagtgaa atttcagaaa tatcaataat attatataat
      541 aaaacaaaaa aacactatat atattcgata aaataaaatt taaacatata atattttatg
      601 tatatattaa taaaaggaca tattgtgtta taatggtatc gtataaaatc atttacgggg
      661 gtggtattaa aatgtttcaa tttgaaacac aaatacacaa attaagacat gatgttttaa
      721 ctgcaatagc tagacttgga aaagaagata accttaccag agaagcaatg gataatattc
      781 agtatgaaat aataaaaggt gagaaaccta catacagatg ctgtgtttat aaagaaagag
      841 ctataatatc tgagagagta aatgtaagta tggggttaca accaggtcaa gataagatag
      901 atttagatac aatggatata gaagaagatg aacaaattat atatattttg gaagctgcat
      961 gtgacagttg tccaataaat gaatttacag taacagatat ttgtagagga tgtctagcac
     1021 atagatgtaa agaagcttgt aaatttggag ctataacaca tgtcggagga atggcctata
     1081 taaatcatga attatgtaag gcttgtggaa tgtgtaaaaa agcttgtcaa tatgatgcca
     1141 tctcagaggt tgttagacca tgtaagagtg tttgtccaac taatgcctta ggatttgata
     1201 gagaaaatat gaaagctatg atacatgagg ataaatgttt aaactgtgga gcatgtatgt
     1261 cagcatgtcc atttggagca atatctgata agagtttgat agctcctgta gctagaaagt
     1321 tagttcaaaa agagaaaatg tatgcggtgg tagctccagc tataacaggt caagttgaag
     1381 caaatgttac atatggtcaa ataaaaaatg ctataaaatc actaggattt gttgatgtat
     1441 tagaagcagc ttgtggagca gatgcagtta cagttcatga aagcttagaa tttgctgaaa
     1501 gaatggaaaa gggagaagct tttatgacaa attcttgttg tccaggattt gttggttata
     1561 tagaaaaaac atacccaaat catgtaggaa aaatatcttc tacagtgtct cctatgatag
     1621 ctagtggtag atttataaaa agtcatgaac cagatgctaa agttgtattc ataggaccat
     1681 gtacagccaa aaagagtgag gcagctaaga aagaattaaa aggagctgtt gattatgtac
     1741 ttacttttga agaaataatg gcattatttg aggcatttga tatagacttg gctaagtgtt
     1801 caaatgaaga tatagatgat gcatcaattt ttggaagagg ttttggagct gctggtggtc
     1861 ttacaaaagc tatagaaaat tatcttgctg aaaaaggtat tggtgttaat tttgaacctg
     1921 taaagatttc tggttcaaga gaaatcaaga aaactatgac tatggctaat ttaggaaagc
     1981 tagaaggtaa ttttatagaa ggaatgatgt gtgaaggtgg ttgtataaat ggttctggta
     2041 aattcttatc aggtgcaaag gttaaagcca cttttgatag aaaaaattct caatcaacta
     2101 agaaaagtgt cttagcaaat acaaagataa aagattttag taatataaat ttagaaaaat
     2161 aaagataata caaagcaaca aaaatacata ttgttaatag agttgttcta agttgaggtt
     2221 taacttttgg acaactctat ttttatttat atatttaatt attgaacaac taaaataaaa
     2281 attatataca cttaaaataa aaattacata tataatattt tgtatataac ttaacaaagt
     2341 atatgcctat gttataataa tttatagaaa taaataaaaa atacaatata cataatagta
     2401 gaaatatagc aaaaaggtgt gatttatagg gaggattact tatgtttgaa tttgaaacgc
     2461 aactaaaaag gttaaaacat gaagttttaa cagcagtagc aagactatct attgaagaaa
     2521 atttaacaaa agaaaatata gaaaaaattc catatgaaat aattaaagga gaaaaggcga
     2581 gatatagatg ttgtgtttat aaagagagag aagtagttct tgaaagggcg caacttgcat
     2641 taagtttaac tccaaatagt aaatatggag atataaatcc agattcagtt agtttggaca
     2701 cagatgagca aattatatat atagttgaag cagcatgtga tagatgtcca ataaatgaat
     2761 ttacagttac agagctttgt agagggtgtt tggctcatag atgtaaggaa gcatgtaaat
     2821 ttggggcaat ttcttatata aatgggagag catatataaa tcatgattta tgtaaggctt
     2881 gtggaatgtg caaaagttca tgtcaatatg atgctatatc agaagttgta agaccttgta
     2941 aaagcgtttg tccaacagga gctttggatt ttaatagaaa cactatgaaa gcaatgatac
     3001 atgaagataa ttgtataaat tgtggagcat gtatgtcagc atgtccattt ggagcaatat
     3061 ctgataagag tttgatagca cctgttgcaa aaaaacttgc aaagaaagaa aatatgtatg
     3121 caatagtagc tccagctata acaggtcaaa tagatagtaa tattacatat ggtcagttaa
     3181 aaaatgctat aaaatcactg ggatttgtag atatgataga agcttcttgt ggagcagatg
     3241 cagttacagt tcatgaaagc aatgaactta ttgaaagaat aaaatcagga gataattaca
     3301 tgacaaattc atgttgtccg ggatttttta agtatataga gaagatgttc ccagatgaaa
     3361 aagatagaat atcatctaca gtgtctccta tggtagcaac aggaagatta gtaaaaaaaa
     3421 tagattctga tgctaaagtt gtatttatag gtccatgtac agctaaaaag agtgaggcaa
     3481 cagagaaaag tttaaaagat tcagtagatt atgtacttac ttttgaagaa ctaatggcat
     3541 tatttgaagc atttaatata gatttatcaa aatgttcaaa tgaagatata aatgatggtt
     3601 cgatttttgg aaggggattt ggagcatctg gtggacttgc caaatcagta cagaactata
     3661 ttgctgaaaa ggggcttagt tttgattttg aacctataaa agtatctggt ccaagacaaa
     3721 ttaaaaagac aatgacaatg gctaaattgg gaagattaga aggtaatttt atagaaggaa
     3781 tgatgtgtga agaaggttgt attaatggag caggtaagtt tttatcaggt gtaaaagcta
     3841 aaaatacttt tgatagaaaa aatggacaat ctactaaaaa gagtgtttta tctaatgata
     3901 agattaaaga atttgaagat gtaaatttag aaagataatt gttttggtac agtcattttt
     3961 ttatcatgcc acaaatatgc ctaaaatttg ccctttttat atgtgtattt tacagagtta
     4021 aggttgctat actataaata tgtaataaat aaaatttata ggagcttttg gatgaggaaa
     4081 atagtattaa aagatatcct gtttttagtt actacattat ctataatagt aatattattg
     4141 cttgtaacaa atataactac taatagtaag ataaaatcaa cgaatgaatc ttatttaaaa
     4201 agtgacaaaa atgatgtgag tcaaaataat attcagagta gcacaaaaaa tactgcattt
     4261 aagactacta tattaagtac tggaaagtct gactgtatat tggttgaaat aggtaacaaa
     4321 gtcataatga ttgatacagg agaagataaa aatggaaaac aaatagtgga taggttaaaa
     4381 gaaaaaggaa taaatacact tgactattta attttaactc atttagataa ggaccatata
     4441 ggtggagtag atagtgtgtt atctagtgtt aaagtaaaga atattataca agcaaattat
     4501 aagaaagata gtaagcaata tgatgaatat attgattcac ttaaaaaagc tgatatagaa
     4561 ccagtgctac taaaagataa tatgaatatt gtaatcaata gtgcagaaat aaacatacat
     4621 ccggcttcaa aaagcaagta cgagagttca aatgattatt caataatcac taatataagc
     4681 tatggagcac ataagttttt atttgcagga gatgcagagg aagaaagatt gtcagagttt
     4741 ataaatggaa atactttaaa atatgatttt gttaaaatgc ctcatcatgg aagatatgac
     4801 aaattaactg aaacattttt agagtctatt tcaccccaat atgctgttat tacatgttca
     4861 gagaaaaaag aaccagagga aggtgtttta aaaatattgg aaagattgaa tataaaaaca
     4921 tttttgactt ctaatggaga ggtagttatc aatagtgatg gtaagacatt gagtgtgaat
     4981 caataatttt tttaaaaata tctaaaaaaa tttaaaaaaa tcattagcaa atattacccc
     5041 ttaaacatag tatattttag gagctgaaac tccacataca aaatgactag gaggtaacat
     5101 attatgttgg atagattttt tggtgaaggc ggatttggtg gatgtgcttg gtggataata
     5161 atattattct tcttattctt agcattccaa gattgttggg ctgaaatgga cttatgtgca
     5221 tggatacctt tcttaatatt attattaata ctttgtgaat gtggtgacgt ttttgatgga
     5281 ggatgtggat gctaaaaaaa tcaatttaga tgatttaata aagcttatgg tgggtataaa
     5341 taaaatacca aaccaatcac aatctagtgt ttgaaggtag tcggaaatcc cccgctacct
     5401 tctttttttt attgttaaaa tttatttttg aattatttca tttacatata ataattttta
     5461 cgataaagtc tgatataatt aaaaagaaaa taaaatttat ttataggaga tgtaaaaatg
     5521 gctatagtgg ataagatttt caatgagata aaacttatgg ttgcaagact tttattggga
     5581 aaaaaataca gtgaatatga agaaaaagat ttagtgtata atacagaaga agaagttata
     5641 cttataactt taaaaagatt agtttttcag ggaaatgtaa acgaagcaga agaaatttta
     5701 tttgataaag ctaagagtgt aaatagtgaa aatatgcagt atatagcaat agaattttat
     5761 actatgctta tggaaaaaac tgatgaagaa ttagaagcta tgaatttttc taaacaagaa
     5821 gtttatcaag gtatagaaga tattagaaaa gtattgaatt tagaataaaa gatatat
//
LOCUS       DACULQ010000205         5854 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.207, whole genome shotgun sequence.
ACCESSION   DACULQ010000205 DACULQ010000000
VERSION     DACULQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.207"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            155..967
                     /locus_tag="JA154_16490"
     CDS             155..967
                     /locus_tag="JA154_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT7997429.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            977..1768
                     /locus_tag="JA154_16495"
     CDS             977..1768
                     /locus_tag="JA154_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter permease"
                     /protein_id="HAT7997430.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            1770..2465
                     /locus_tag="JA154_16500"
     CDS             1770..2465
                     /locus_tag="JA154_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997431.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2452..3183
                     /locus_tag="JA154_16505"
     CDS             2452..3183
                     /locus_tag="JA154_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997432.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNSHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3497..4297
                     /locus_tag="JA154_16510"
     CDS             3497..4297
                     /locus_tag="JA154_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HAT7997433.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4836..5552
                     /locus_tag="JA154_16515"
     CDS             4836..5552
                     /locus_tag="JA154_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7997434.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            5533..>5854
                     /locus_tag="JA154_16520"
     CDS             5533..>5854
                     /locus_tag="JA154_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7997435.1"
                     /translation="MKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSNINYYY
                     REFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVKFLENV
                     INLFS"
BASE COUNT         2274 a          712 c          835 g         2033 t
ORIGIN      
        1 cctatggaaa tgacattatg aatagatttt ttcttaacta tacataacaa aacataacaa
       61 aacattataa aacataacaa aaacttagac atcgttttct tagtatgata tactaaaaaa
      121 gaatttacac taatttaata agggggaata tcttatgttt aaaaaacttt cagtactagc
      181 tttagctatg attatttcat taagtattac tgcatgtaat tctaaagaaa acaaagctaa
      241 tgatgaatct aatactaaag tgacaacagc tgatgattca aaagaaaaag agactctttt
      301 tgtctattca ggggcaggtt taaagaaacc aatggatgaa atagcaaaaa aatttgagaa
      361 agaaaataat gtcaaagttg aatatagcta tgcaggttct gcacagctaa tagctcaaat
      421 tgagacaagt cacaaaggag acgtatttat tgttggttct gaaccaattt attcaaaggc
      481 ctttgaaaaa gaacttgtag gtgaacataa aacggttgca catcatacac ctgcaatagt
      541 tgtacctaaa ggaaatccag ctaatattca aaaattggaa gacttaaata aagatggagt
      601 taaacttatt ttgggagaca aagagtctaa tgctataggt aagactacac aaaaaatact
      661 tgaaaaaaat aatttaaaat ctataaatga taatgttgtc tctacagctg caactgtaaa
      721 tgaaatgatt gtacagttaa catcaagtaa agctgatgct acaatagcca caaaagattc
      781 ggtctttggt aatgaagatg tagaagttat agaaattgcc caagataaaa atatagacca
      841 actcatatca gcaggaatcg taaactatag tgataaaaaa gatttagcta ataaatttat
      901 aaattatata gagtcagacg aagctaaaca gatatttaaa aaatatggtt ttgaacctgt
      961 taagtagtga ttaaacttga aaaataaatt ttttactact ctacttactg gattttttgc
     1021 tatagtcttt ttgtttattg tatcaccact tcttatgtta atcattaaag ggataagtta
     1081 tgttcctata tgcttaaaat cagtggaagt acagtatgca ataacactta gtattaaaac
     1141 atctttaata tctactatag tatgcctttt attagctatt ccagtagcat attttttaca
     1201 tattactaaa ttaccattta aaaaactgat tatacagata ataaatctcc ctatgtctct
     1261 tccacactta gtatcaggaa ttgctctttt acttttgttt ggaagaatgg ggattggaga
     1321 ttccatttat aagattttta agcttgattt tatatttaca aagcaaggta tagttttagc
     1381 acaaatattt gtaaatcttc cacttactat taaaatactt cacacttcat taaatgaaag
     1441 taatgaaaag atgatatttg ttgcacgtac ccttggatgt aatagttggg aagcatttag
     1501 atttataatt cttccaaatc taaaaactgg aatcatatct gcaactgtaa tgacttggtc
     1561 aagagcactt ggtgaatttg gtgcagtagc tatgatagca ggttctacac gtatgaaaac
     1621 tgaaattatt cctacatcta tatatttaaa tatgtctact ggtgatatag acattgctat
     1681 cggaatagct gtaattttga tatttctctc acttacatgc ttgatgcttt ttgagatatt
     1741 ttttaataga gaggttgata agaattaata tgttaaaaat aaaaaattta tctcttgatt
     1801 tacctaattt taaattacaa gaaaatagtt taaatattaa taatcatgaa tactttgttt
     1861 tattaggagc tagtgggtca ggtaaaactc tatttctaga aacattagca ggaagatatc
     1921 catctgctaa aggaaatgta tcatacaaag aaaatttaat atcaaactta cctccagaaa
     1981 atagaaatat aggatttgta tatcaaaatt ttgaattatt tccaaatatg aatgttgaag
     2041 ataatataag gtttccactt aaattgagaa aaatacctat agatgaacag attcttaagg
     2101 ctaaccactt gtctaaatta atgcagatag aagatataaa aaataggtat cctaaaaatt
     2161 taagtggagg cgaaaagcaa cgagttgctt ttgcacgtgc cttaatagct gaacctgata
     2221 tattactttt agatgaacct atgagttcat tggattatat tacaaaaaaa catataagca
     2281 aaattttgaa ggatgtctac ataaaataca aaccaacagt gattcatgtt actcatgata
     2341 tttctgaagc aatgtttttt gctcataaga taggaataat gaacaatgga aaaataaatc
     2401 atatttttga tttaaatgaa gaaataaaaa ataaaggaga atcttttttc tatgaatata
     2461 tttgatattt tgagagatta tctattagta caagtcgata aatataattt aaatatggat
     2521 atgataagta ttgtgtccaa atctctatct tcaaaagaag caattggaaa tactaagcgt
     2581 aaagatttcc caattatagt tggtaaagaa ataatgcttg aagctgactt taaaggagct
     2641 aaaggacaag catttacttc tactccatct acatttgaag gttctttaaa agacatatta
     2701 tcattagatt tacatgacaa ttctcatgat agaagtcttt ttatagcatc attaaatgca
     2761 gttatgaaat acttaggcaa gacggataga accattcatt gtaaaaataa cgagccagag
     2821 gtatgtgcaa aaaaattccc tgagtttata aaaatggaat ttggtaatcc aaaagtagca
     2881 attataggat atcaaccagc cataattgat aatattaagg actttttcga aacaagagta
     2941 ttagatttaa atcctgaatt tgtggatact attcaatata atgttaagat tgaagatggt
     3001 atacgagatt atgaagatgt aatatcttgg gctgatttag tgatttgcac aggaagtaca
     3061 ttatgtaata actcaattat aaattttcta tcacttaaca aaccagttta ctattatgga
     3121 actacgatag ctggtgcatc aaatatttta ggtttaaaaa gactctgttt ttgtagtaaa
     3181 taagcaatca tatcatcaag gagtacacaa atatatatgt gtactcttta aatttaaaaa
     3241 gttattactt ttattctaag tactaaagta aaattctatt aaaatgaatc ccgaataaat
     3301 gtacaatccc tttaaatctc aattcctatc aatcattaat attttgatta gtcttataca
     3361 taaatgtttt ctttatttta aactaataaa aaaattttat tccattaatc tactctatat
     3421 ttttcagcat ttaagaaata atttttagta ctttgtttga taatataact aagacaacct
     3481 ttcaggagat gattaaatga catttaagaa taaaaaattt actataaata aatataaaat
     3541 tctatctatt attctagttt tatgtttgat tttaaccgct tttagtagct atgtgaatta
     3601 tagtcataaa aaatttaaag attatgttgt cttctatgtt cagcatcaag atgatgaagt
     3661 gttgtgggct gggagtgcta ttgttaatgc tataaaagaa agaggaaaaa atcatgtatt
     3721 tgtggtttta gtatctactg gatgtggtat atccgtcttt gataaatata aaaaatatga
     3781 aaacctaaca aatatacaaa aatcagaata tagaaataga gaatttttag cttctcttaa
     3841 tagccttggt gtaataaggg aaaatataat attattacca gaaacaaata caaatggtaa
     3901 tactgacttc aattacatgg aaaaaattgc tcttacattt gaagaaaaat ttaaaaatgt
     3961 aactcatata gcacatactt ataaattaga tgaccattta caacatttaa aaaatggttc
     4021 tgttatccaa aatttatata atgctggtaa aataaaagat gctaaatatt ttgtaaaacc
     4081 taaatttgct gataaaataa cttttaataa taagatagtt tataaagctc attctactga
     4141 ggactatgaa aaggttaaaa atgcctgtca acaatacaaa attgttgatg aattagaata
     4201 tagagaaggt attggctata agtctgacca taaatcattt gataagttat taaataataa
     4261 agatgttcct gctatacttc atacacctaa tttataagaa atatttattc tttaatttaa
     4321 gttataaaaa ggaaattatt tgagattttg tgatatagca ctttttgtgc tatactactt
     4381 tttctcatac aactattacc catatctaat ttcatcatct cttaaaatat ataaagatat
     4441 catcattaaa acctataagc cttattaaat gctttataca tacctacatt aaactacctg
     4501 tttatattaa actacattaa aataaatatt tgttataaat tcttaataat tatgaaactc
     4561 atttgttatt tatatttgtt aatatatgtt taaacctaaa aaattaataa accaaaataa
     4621 cataagaagt gtgatagatt accctagata ttttcataaa cttttacctt cagattaata
     4681 tgggcgtcta tacttttaaa tttactatat gcattgacaa tatattaaaa gtactagttt
     4741 ttatttcata atgaacgggc ttactgtttt ctaatacctc tttttggtat aaaataaaaa
     4801 ttggttaaaa ataaaatttc aagggggtta ataaattgaa tcaaaatata ttggttgtag
     4861 atgatgatag agagatagtt gatgctatag agttctattt gagacctgat aactttaatg
     4921 ttttaaaggc atatgatgga cttgaagcat tggacatact aatcgaaaat gatattaaat
     4981 taataataat ggatgtaatg atgcccaatt tagatgggct taaaactaca cttaaaataa
     5041 gagaaaaaag gaatatacca ataattctct tatctgcaaa atcagaagat atggacaaaa
     5101 ttataggtct taatatgggt gctgatgact atataactaa accatttaat ccactagaac
     5161 ttactgctcg agtaaaatct caacttagaa ggtatataaa tttaggtgat tttagtaata
     5221 caaataactc aaatgatact aattatattc taaaaagtgg tggcctagag ctaaatacag
     5281 atacaaaaat agtttcttta gatggtgagg aaattaaaat gacaccactt gaatataaaa
     5341 tacttacact tcttatgtca cgaaaaggta aaattttctc ttctcatgag atttatgaaa
     5401 atgtctggaa tgaggatgct tatagttgtg agagaactat tgctgttcat attagaagaa
     5461 ttagagaaaa gattgaaatt aatccaaaag aaccaaaata tttaaaggtg gtgtggggca
     5521 ttggttacaa aattgaaaaa cttaattatt aatgattcta aagcaaaatt tattttatta
     5581 ttattactgt ttctattagt ttctatatta tttgaagcag tttatgattt gaataataac
     5641 ttttctaata ttaattatta ttatagagag tttgctctcc cttattttta ttttttcggt
     5701 actcctgtac atttgtcaaa agtagaatta gagtttgtct gcataggtat tatattatca
     5761 agttttttat tgactatact atgttttaca ttgtataaaa aaaataaaca aactaaagtc
     5821 aaattcttag agaatgtcat aaaccttttt tcta
//
LOCUS       DACULQ010000206         5744 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.208, whole genome shotgun sequence.
ACCESSION   DACULQ010000206 DACULQ010000000
VERSION     DACULQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.208"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1098
                     /locus_tag="JA154_16525"
     CDS             <1..1098
                     /locus_tag="JA154_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT7997436.1"
                     /translation="YKFRLYPNKKQQELINKTFGCCRFVYNKYLAKRIDVYKNNKETF
                     TYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKFKSKKINR
                     FSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPQGRILNATISKEPSGRYYVSLCC
                     TDVDIEAFENTNNQIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQRELSRKTI
                     GSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNMIKNHKLS
                     RLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWI
                     CPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            1282..2394
                     /locus_tag="JA154_16530"
     CDS             1282..2394
                     /locus_tag="JA154_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAT7997437.1"
                     /translation="MESVAISGVFAPVNKQQEEEVAKWVREILGDIPISLSNEIGNIG
                     ILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIYFSQNDGTLMNLEYTMKYPI
                     FTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLYNGFPRESSIPVTIGGVHTN
                     FRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDAIIFGGDTLTLSDVVTKLDM
                     AFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQDDVELVLTGGGSIIVPEKL
                     KGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMSREDAIEDAKKEAVNKALKA
                     GAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(2557..3099)
                     /locus_tag="JA154_16535"
     CDS             complement(2557..3099)
                     /locus_tag="JA154_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HAT7997438.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(3247..3531)
                     /locus_tag="JA154_16540"
     CDS             complement(3247..3531)
                     /locus_tag="JA154_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997439.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(3594..4358)
                     /locus_tag="JA154_16545"
     CDS             complement(3594..4358)
                     /locus_tag="JA154_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAT7997440.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(4640..4906)
                     /locus_tag="JA154_16550"
     CDS             complement(4640..4906)
                     /locus_tag="JA154_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HAT7997441.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(4948..5703)
                     /locus_tag="JA154_16555"
     CDS             complement(4948..5703)
                     /locus_tag="JA154_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7997442.1"
                     /translation="MPHKLLRQYIAHYTISIPDTSIKENLTLIPDASGCMIFKFDKKG
                     IESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTGLSQRESTDLKISLEDFNT
                     LFSLEVNSIFERTSTIEELVEQLDMLFLSYLLKSNIVDMTIPILENARKQNSIMSVKN
                     ISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEIQNNKIPFATLAQDIGYYD
                     QSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         2060 a          766 c          823 g         2095 t
ORIGIN      
        1 tataagttta gactgtatcc aaataaaaag caacaagagt taattaacaa aacttttgga
       61 tgttgtagat ttgtatataa taagtatctt gctaaaagaa tagatgttta taaaaacaat
      121 aaagaaactt ttacttataa acagtgtagt tctgatttga ccaatttaaa aaaagaatta
      181 aaatggctta aagaacctga caaattttca ctacaaaacg ccttaaaaga tttagataat
      241 gcatataaaa aattctttaa agaaaaagca ggatttccta aatttaaatc aaagaaaatt
      301 aatagatttt catataaaac taactttaca aatggaaaca ttatgtattg tggtcaacat
      361 ataaaactac ctaaacttgg tatggttaag ataagggata aacaagtacc tcaaggtaga
      421 atacttaatg ctactatatc taaagagcca agtggtagat attatgtatc attatgctgt
      481 actgatgtag atattgaagc atttgaaaat actaataatc agataggttt agatttagga
      541 attaaagaat tttgtatctc aagttgtgga gaatttatag aaaatcctaa gtatcttaag
      601 aagtcattaa acaaacttgc taaattacaa agagaattat cgagaaaaac aatcggtagt
      661 ttaaatagaa ataaagcaag attaaaagtt gcaagacttc aagaacatat agctaatcaa
      721 agaaaggatt ttctacaaaa actatctact aaattaataa aagagaatga tatcatttgc
      781 atagaagatt tacaagtaaa gaatatgatt aaaaatcata aactatctcg cttaatttca
      841 gatgtatcat ggtcagaatt tattcgtcaa ttagaataca aggctaattg gtatggtaga
      901 caaattgtaa aggtaggtaa attctttgca agttcacaaa tatgcaataa atgtgggtat
      961 aaaaacgagg aagttaagaa cttaaatata agagaatgga tttgtcctag ttgtaatgaa
     1021 actcacgata gagatataaa tgcaagtata aatatattaa aagaaggatt aagactaata
     1081 acaattcaaa ataaataacg tatgtaagaa ccggaggaac tacggggata gcctgtggag
     1141 aattagtaag acatatttta gtatgcaaaa ttctgtgaag caggaaccct gtgactttag
     1201 tcatgggagg ttcagggtag aaagataaat gaactttgta aagatgaagt tgtagagttt
     1261 tgcaatagaa taaaaggtaa agtggaaagt gttgcaataa gtggagtatt tgctccagta
     1321 aataaacaac aagaagaaga agttgcaaaa tgggttagag aaattttagg ggatattcct
     1381 atatctttat caaatgaaat aggtaatata ggtatattag aaagagaaaa tggagctatt
     1441 ttaaattctg cattgtctca agtaggtaaa gctgtatctt taggatttga aaaagcactt
     1501 aaagacttag gtatagatgc tcaaatatat ttcagtcaaa atgatggaac attaatgaat
     1561 ttagagtata caatgaaata cccaattttt acaattggat gtggaataac aaattctata
     1621 agaggagctt ccttcttatc aaaagtaaaa aatgcaatag tattagatgt tggtggaact
     1681 acaagcgact tgggtgtact atataatgga ttccctagag aatcatctat tccagttaca
     1741 attggaggag tacatacaaa ctttagaatg ccagatgttt taactattgg tcttgctggt
     1801 ggtacatgtg taaaaggcga aaaagataat ataaagatag gtcctgaaag tgtgggatat
     1861 agaataactg aagatgcaat aatatttggt ggagacactc taactttatc agatgttgtt
     1921 acaaaattag atatggcgtt tgaagataga aaaagcaatg ttgaaaactt agataaagag
     1981 tattgtaatg aggtatataa gaaggttatt ttaaaattag aagatgctgt agaccaaatg
     2041 aagactagtc aagatgatgt tgaattggtt ttaactggtg gaggaagtat aattgttcct
     2101 gaaaaactaa aaggcataag caatgttata agacctcctc attatactgc tgcaaatgct
     2161 attggtgcag ctttaggaca aataagtgga gatgtagaaa aagtatacag tttagataaa
     2221 atgtcgagag aagatgccat agaagatgca aaaaaagagg ctgtaaataa agctctaaaa
     2281 gctggtgcta aagaagatac tatagaagta ttaaatatag aagatgttcc acttgcatac
     2341 cttcctggta atgcacttct tataaaagta aaagttgttg gtgatttaat ttaaagaata
     2401 aatataagta tttccagaat tatataaata tttctggact catttttaaa cttattttat
     2461 caataaaaac agcataactt ttgactatat tcttataagt ttccgaacag ccgatatata
     2521 cctatttttt gaggtttatc ggctgtttgt tatcttttat cgcattccaa ctatttttct
     2581 taccgtattt gctgtacgaa ttgtaatctt atcacttaca tttgaactgg cagtctttga
     2641 ccaccaattt gtctttttat aatctttacg actaaaagac caaaagatag catttttata
     2701 atgatatact ttttcatact cttctttagg atttccaacc tcatcataaa actcgtcata
     2761 agataagtca ggaaacataa aaataagatt atcatatatt tctttgttgt tatctcccca
     2821 ccaattagga gcattattta gtatatcttt taactcttct tgtgaaataa taaaaactgg
     2881 tatttccaat tcaaactgtt tttttatcat taatcttatt gtatttgaga taaccttgct
     2941 gtcatctaca tcacttaaaa agacaacatt accactatta aggtatgtag aaacttctga
     3001 ataaccaagt tctacaaagc ttgcttttaa ttcagacata attatcttgt tcttaccact
     3061 tatattgata cctcttaata atgcgatata acttttcata taataatttc acctctaaac
     3121 attcacattt ttctatgtag tacaaactgg aatttgcatc ttttataatt ctttcaaaac
     3181 ttcttcatat gtaatttttt taatataaaa aaatcggtgc ttaatttgta ccgatttttg
     3241 tttttcttag taaagaactc gtttttttgc ttctgctata gctctagcag cactttttgc
     3301 ttgcattctt ttttcttctt cttttttaaa ttcatttatt gtttcattta gattcttatc
     3361 atgttcattt ttttctttat cttcgttttc tctgtatact aatatataat ctattattga
     3421 ttcgtttatt atttgagata tacttttatt ttcattaatt cctattctct taacttcctt
     3481 aagtagagtc tcatcaatat gaaccgttgt ttgctttgta ttttttgcca ccttaatcac
     3541 ctcttaaaaa aaattctaac attaaaattt tttttagtca ataaatttta atattaaaat
     3601 tctttaaatg ttttatttga ttttctaaaa ttttctattg tatttatcat cttatttttt
     3661 atattttcac ctagtacaga agactttagt ctaggatttt cgatatggtc ataaattggt
     3721 tctaaaaatt cgacttcgac aggaacagag ttaattttac caataggttg gtatccttca
     3781 taagtatctt ttgaattttt tattactagt ggaacaatag ggcattttgc tttatatgct
     3841 atctttaaag ctccacttct aaactccgaa accaatgaat ttggttcttt aatccatgtt
     3901 aaatctcctt ctggaaacac tgccatactt tgacctgtaa gtatattttg agaggcttgg
     3961 gctatactct ttataccctc acgattgttt tttctgttta catatacaca tcttagtaat
     4021 tttgcccatt ctttaaatat aggtatattt ctccaaactg gttcatctgc aattacacat
     4081 ccaattggtc tttctacact tgctgttagt atgaagctgt caagcatact agaatgattt
     4141 acaacaaata gaagtggttc ttttggcagt gtttccttac caattatatt aagctctatg
     4201 ttcactatat ctaaagattt tttagcttgt ttttgcataa attcaaattt ttcctttaag
     4261 ctaaatttat caggattctt tttaatttta ataagcctag gtatagaaga tgtaaaacct
     4321 ataagttgat atgtagcaaa ttttaagtaa ttattcataa aattatctcc ttaaaataaa
     4381 attatttatt actgtaaaga ttttaacata aaataaaatt taagtctatt ttaattcaat
     4441 tttgtaacat atattttaaa tagacagaga ttgagatttt atatttccaa ttttagtaat
     4501 cagattttat caataaatta ctattctatt aatttaatta aataaccttt aaatttaatt
     4561 ataaaaactg ttgcagaaat ttctacaaca gtttaaaaaa tttaccttac aaaaacttat
     4621 tttataacaa gagagtatat tataaggcta gtacagccca ttttttgcac tgagaaattc
     4681 ttttgccatc atcaccttgt tctttgtcat atgcaaattg attaagcatt tcacatggaa
     4741 atgtatcaca tagaccacaa tgtgtaaggt ttttatcttc acaacaagat tttacaggac
     4801 attgttcacc ccaaaatggt ttctcaatct ctgtacaacc tttacaattg actttttcct
     4861 tgtatccaca ttcataacat aaaattccac atcttgattc tatcatttta agtcactcct
     4921 taaatataaa ttttatatga ttgtatatca aaatttatac ttttcattgt aaaaatcgga
     4981 caaatttttt atataggttg ttggattaac accacaaata gatttaaaat catttataaa
     5041 atgagattgg tcgtaataac caatatcttg tgctaaagtt gcaaatggta ttttattatt
     5101 ttgtatttct tgtaatacaa ggtttatacg aagtaatcta aggtatgatt taacactcat
     5161 accaagagaa ttattaaaaa ttctatttaa atgtcgttca ctataacaac taatttgtga
     5221 aatgttttta acagacataa tagagttttg ttttctagca ttttcaagaa ttggtatagt
     5281 catatctact atatttgatt tcaataagta tgataagaat aacatatcta attgttctac
     5341 taattcttct atagtacttg ttctttcaaa aatggaatta acctctaagc taaataatgt
     5401 gttaaaatct tcaagtgaaa tttttaaatc tgtagattct ctttgagaaa gacctgtcaa
     5461 ataatatact ccacctgggc gaaactcaac gaatactcta aaaagtacat tttctatatc
     5521 attttttaca atagtagttt ttgttgtggc tccccagaat gcgctttcta tacctttttt
     5581 gtcaaattta aatatcatac atccactagc atcaggtatt aatgtaagat tttctttaat
     5641 acttgtatct ggaatagata ttgtatagtg tgctatatac tgtcttaaca atttatgtgg
     5701 caaaatatat gtatactgtt tgcaggtatt taaaatgtta ttga
//
LOCUS       DACULQ010000207         5685 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.209, whole genome shotgun sequence.
ACCESSION   DACULQ010000207 DACULQ010000000
VERSION     DACULQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.209"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(279..800)
                     /locus_tag="JA154_16560"
     CDS             complement(279..800)
                     /locus_tag="JA154_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAT7997443.1"
                     /translation="MKFKRLRTSENKLCYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            1156..1953
                     /locus_tag="JA154_16565"
     CDS             1156..1953
                     /locus_tag="JA154_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997444.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGIKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            2063..2653
                     /locus_tag="JA154_16570"
     CDS             2063..2653
                     /locus_tag="JA154_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HAT7997445.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            2719..4416
                     /locus_tag="JA154_16575"
     CDS             2719..4416
                     /locus_tag="JA154_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAT7997446.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            4630..5469
                     /locus_tag="JA154_16580"
     CDS             4630..5469
                     /locus_tag="JA154_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAT7997447.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         2228 a          578 c          940 g         1939 t
ORIGIN      
        1 tattattaca caaaaataga aacctaaatt tataaaaata gaataaagtt tgttatataa
       61 aatgtaattt tgtctataaa atctcacttg ttttcttaac tttaagcgtg aaaaagagaa
      121 tgtatcatga attttttagt tcatgatgca tccttctttg tatgaaatcc aatatatctt
      181 tattatttaa acaatggaaa agagccttat ttctattttg agacatctct tttatttaat
      241 tatgaagttt tatacagttc aataaatagg aattatattt agaaactcat gacattattt
      301 cttagatagt gattaaattt atcatattcc attttagata acttttctgg gtaagacatt
      361 acaatcaggt tatgcccatt aaatttttca tggtatggtg gatgaatgtg ttcataatta
      421 tttgtcttat attttactct ttcatagaac gccttgcgat aaattgaaat ctcatcaatt
      481 ggaaggtcaa tttctaaaat aatagttttt cctttcttat ttaaaagctc taacgctctt
      541 tgtccatatc tattattgcg catttctgga aaaatgcaga aatgttctac atagataaag
      601 tcatctgttt cccaacataa aattatgccg acaaactggt ttttatcata aattaaattg
      661 aactgatatt ctttattttt caaaatttct tcttgcaaag aagattttct ttgttcatga
      721 aatgggaaac taattttata taattccata gccttgtaac ataatttatt ttcactggtt
      781 cttaaccgtt taaatttcac tttactcctc ctaatcacga aaattccaat ttatttatga
      841 gttcatttta ccatagtcag tatttgcata caatgtgttt taagttgagt gtcatttctt
      901 tcttttattt atgcttatat ctcatagact aagagataat tgcttaagac ataactaaaa
      961 actattatat taagctttga taatctaaaa acagtatatt tatggacttt tctaggtcta
     1021 aaagtaatct taataatttt agaagttaca aaaaagttac ataaatttgg aaagctataa
     1081 tatacatata taatataagg ttaaaactgt aatgaataat attaatttaa aatttgaaca
     1141 tggaaggaga ttattatgga gaaaagagaa aagaggataa taatatcatc tttattaagt
     1201 gtatcaattt taatgggttt ggtaagtata tattctatat taaataaaga agacataatt
     1261 ttaactgtta agggtcaaga gcaaaaagta tcttccttta agaaaacagt tgaggaactt
     1321 ttggatgaac agggtataaa gtataattct gaagataaga ttaatccaag tttagataca
     1381 gaactaaaag atgatatgaa aataaaagtt gttaaggtaa ctaaaagtaa aaaagaagag
     1441 attgaaaaaa ttccatttga tacaaagcat gtaaatgata gtaatttgtt aaaaggaaaa
     1501 tctaaagttt atcaagaagg tcaagaagga gaaaaaaaac tagtctataa tttaacttac
     1561 catgatggaa agttagtcaa aaaagtctta tcaaaagaag taatatctaa ggagccaact
     1621 acaaaaatta taaaatatgg aactaaagaa aaagtactaa tagcatcaag aggagcaaat
     1681 ataagaggag gcaaacatat gaaagtggtt gccactgcat atgcaggaga tacaataaca
     1741 tctactggta caactccaag atggggtgtt attgcagttg acccacgtgt aataccatat
     1801 ggaacaaaag tatatatacc taaactgggt atgacttttg tagcagaaga ttgtggaggt
     1861 gcaattaaag gtaatagaat agacattttt atgaacagtg aaggaaaagc ttcaaattgg
     1921 ggaaggaaaa gtatagatat atatctacat taaattagtg aatagaataa ctttacttga
     1981 aggacaaaaa agtataaaat atgaattaac taaactgtta gattattctt aaaacataaa
     2041 aatatatcta aggggggata agatgaaaat agtatgctta ggagacagtt taacgtatgg
     2101 atttggagta tcacgaagta attcatggac aaacattgta aataaagaga ctcgattaga
     2161 aatagtaaat aagggtataa atggtgatac aacaagtggt atgttagcta ggtttaatga
     2221 agatgttgta aaaaattctc ctgatatagt ttttataatg ggtggcacca atgattttat
     2281 tgctggtgcg ggaaatgaaa ttattaattc taatataatg gctatggtgc atcagtcata
     2341 ttcaaaaaat attataccta taataggtat accattaaaa ccagatattc caaatgttag
     2401 ggaagattgg agttgtttta ctgattttaa tatagtatct caaaacttag aatcatatag
     2461 ttattggata aaaaaatttt gtatgacatt taatactaat ttcgtagatt tttattcaga
     2521 atataataaa aatatggaac tagaaggata taaaaaatta tattttgatg ggttacatcc
     2581 aaccaaagag ggtcatagaa taatggcaga catttttatt aattcaatta ataagtatat
     2641 agaagagtta tagattacat taaaatgctt gtttatgcgt cagttgcaat aaatacatac
     2701 agtagagagg ggtttattat ggattatact aaaacttatg aagagtggat aaagggttca
     2761 tattttgatg aagatacaaa gctagagttg gagaatataa aaaataatga aaaagaaata
     2821 gaggatagat tctataagga tttagaattt gggactgcgg gtcttagagg aataattgaa
     2881 gcaggaacga atagaataaa taagtacaca gttagaagag ctacttttgg attggcaaac
     2941 tatatattag aaaacacaac aaaagaagaa acaagtagag gagtagtaat agctcatgat
     3001 aatagacaca agtctagaca attctgtata gaatctgcaa atactttggc agcatgtggt
     3061 ataaaagcat atatatttga tagtttgaga actactccag aattatcttt tgctgttaga
     3121 agcttaaatg caatcgctgg tatagtcata acagcaagcc ataatcctcc agaatataat
     3181 ggatataagg tatactggga agatggagca caagttatgc cagagattgc aaatgcaata
     3241 acagagaaag taaatagtat acatgattat agtacaatac caaccttaac ggaagctaat
     3301 aaaaatttgg tagttttgtt ggacgaatca caagatacta aatttataga agcagtgaaa
     3361 agccaaatta taagaaaaga tttagttaaa aatgtaggta aaagctttaa gatagtttat
     3421 acacctcttt gtggaacagg gaatgttcca ataagaagag cattgaaaga agttggtttt
     3481 gaaaatataa ttgtagttcc agaagaagaa aatccagacc caaattttgc agggcttgat
     3541 tatccaaatc cagaagagaa aaaagcttta aatagaggta tattactcgc aaaagaaaaa
     3601 ggagcagacc ttgtaatagc aactgaccca gattgtgata gagtaggtgt agcagtaaaa
     3661 actactacag gtgagtatgc tttacttaca ggaaatcaaa ttggtggtat gctgacacat
     3721 tatataatag aaggattaaa agaaaataat aaattaaaag aaaatcctac aataataaaa
     3781 accatagtta catcagaatt tggagcagat atagctaaag caaataatgt tgatgtatta
     3841 aatgttctta caggttttaa atttattggt gaaaaaataa aattatttga acaaaataaa
     3901 aatagaagtt atgtatttgg atatgaagaa agttatggtt acttagttgg gactcatgct
     3961 agagataaag atggtgtagt ttcttcattg ttaatatctg aaatggcagc attttactat
     4021 tcaaaaggta tgagtttata tgatggttta atagaacttt ataaaaagta tggatttttc
     4081 aaagaacaga ctatatcttt gactttaaaa ggtatagaag gtgtagaaaa gataaaagaa
     4141 ataatatctt attttagaga aaatcaaatt gattatataa atagtattaa agtagttgat
     4201 aaaaaagatt ataaaaatgg tatagataat cttcctaaat ctgatgtttt aaaatacttt
     4261 ttggaagatg aatcttgggt agctataaga ccatctggaa cagaaccaaa acttaaattt
     4321 tacatagctg taaaaggagc tagtgatatt gaagcagata aaaagattca aggtttgaaa
     4381 aagtatatag atgacatggt cgaaaaatta aaataaaata ttattatttt ataagataat
     4441 tttgtaataa tatttattta gctgaaaatt ataatttact ttcattgagt agaatatgga
     4501 aatgttagta gaattgttat tttcagcttt tatttgtgtt atttttactt aaataattat
     4561 attatagaat gaatctgagt ataattaata ttgtataatg aaattaattt gatagaaaag
     4621 gggatattta tgtttggtat agataagaaa ttgattgaat atataattgc aaaatccaat
     4681 tacagtgaat taagttcaaa gtcagcagaa gtatctttct tcttaatgtt gtccatattt
     4741 ccttttttaa tatttacaat aagttctata gcttatatac cgattcttca tttaaataag
     4801 tatatagctt tgtttagaaa tatgatgcca gaaggtgctt ttgctgtgtt atcttcaatc
     4861 atagtttctg caatagacaa tagaaacctg aaatttttag cagtcagttt tgtacttact
     4921 atgtggacat tttcaagagc agtaaaagct cttattaaag gaatgaatag agcctataaa
     4981 gttaaagaaa ctaggtcttt ttttaaaatt ttatctatat catttttatt tacaataatg
     5041 cttttggtcc ttattttttt atctatgata tttttagttt acggtgaaaa aataggttat
     5101 tttattttta atcttgtagg attggatgag atatttataa aaatatggga tattctaaga
     5161 tacactgtag gtataataac aataatagtg atatttacac ttttatataa atacactcca
     5221 aataaaaagt taactattaa agaatctgcg ccaggtgcta tttttgctac ttttgcttgg
     5281 tttttagtat catttttata ttcttattat acaaattact atgctaacta tgaagtaatc
     5341 tatggaagta tagctgaaat aattgtactt atgacctgga tgtattttag tagttggtct
     5401 atcgttattg gctatgaggt aaattcaaga ttgtatttta gaaagataag acatgaaatg
     5461 ttaaagtaaa gtatttaaaa taaaagagag tatttagtat tataaatgta attgaattaa
     5521 taaagaaata ttgacattta agttaaaaag tctagaattt ttctgggctt tttttatttt
     5581 tagtataact tgacaaattt tatttaatgg gatagtttta atttataatc atctttaaaa
     5641 agatgactat tgataagtaa gagataaaat tggtaagtat caaaa
//
LOCUS       DACULQ010000208         5561 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.210, whole genome shotgun sequence.
ACCESSION   DACULQ010000208 DACULQ010000000
VERSION     DACULQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.210"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1063
                     /locus_tag="JA154_16585"
     CDS             <1..1063
                     /locus_tag="JA154_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAT7997448.1"
                     /translation="LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKI
                     DGVFAYLDREVKFELQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLT
                     MIDNQLLSIYKNFHSGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPC
                     ETILNYSFNKQDISDEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKD
                     LGNHFRSRLNTEFPWNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQF
                     EEDSKLIIEDIYDCIFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYL
                     EEKEKLMNTFKEKIIQIFE"
     gene            1088..1696
                     /locus_tag="JA154_16590"
     CDS             1088..1696
                     /locus_tag="JA154_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HAT7997449.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            1744..2115
                     /locus_tag="JA154_16595"
     CDS             1744..2115
                     /locus_tag="JA154_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAT7997450.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            2294..2632
                     /locus_tag="JA154_16600"
     CDS             2294..2632
                     /locus_tag="JA154_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997451.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            3098..3601
                     /locus_tag="JA154_16605"
     CDS             3098..3601
                     /locus_tag="JA154_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein"
                     /protein_id="HAT7997452.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            3648..4607
                     /locus_tag="JA154_16610"
     CDS             3648..4607
                     /locus_tag="JA154_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin phosphate synthase MoaA"
                     /protein_id="HAT7997453.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            4611..5081
                     /gene="moaC"
                     /locus_tag="JA154_16615"
     CDS             4611..5081
                     /gene="moaC"
                     /locus_tag="JA154_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088218.1"
                     /note="MoaC; along with MoaA is involved in conversion of
                     a guanosine derivative into molybdopterin precursor Z;
                     involved in molybdenum cofactor biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAT7997454.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            5117..5548
                     /locus_tag="JA154_16620"
     CDS             5117..5548
                     /locus_tag="JA154_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HAT7997455.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         2332 a          462 c         1032 g         1735 t
ORIGIN      
        1 acttttgaga agatatttta ataaaacaga tgacaaaata aactattata ataaagagaa
       61 taagaatatt aaattaaaac tatctgagag gcaacccaat aagcaaaaaa atggatttga
      121 aatatataaa atagatggag tttttgcgta tttagataga gaagtaaaat ttgaacttca
      181 aattaaatct cttgtaaatg tgttttggag tgaaatagaa cataaaataa tttataaaaa
      241 caatacatat ttacttgctg ataaatttat aaaggatatg atggactcta taaagaataa
      301 tcttactatg atagataatc agcttttaag tatttataaa aactttcatt cagggaagtc
      361 ttttaatatg aaagtaagta aaaaagaaat tgaaaaatta tttgcgaaac tagtctatga
      421 cgctttttct gaaaagatga ataaaagtat aggttttgtt gtagatttta aaaaaccatg
      481 tgagactata ttgaactatt cttttaataa acaagatata agtgatgaag ttttaggtaa
      541 ttttatgctt gatgagtttg caagattaaa tgaaattgta aacaaggata tagattttaa
      601 tgagcaaata gaatttgaaa gagaaccaac atttgatgat aagttttgta aagacctagg
      661 aaatcatttt agaagtagat taaacacaga atttccatgg aacctatttt ttagaatatt
      721 atttgaaata gagccatata acaataccaa agactttgaa aactttgtag agtttataaa
      781 agaaaatgtg ctactagaag aaaataggga gcaattatta tgccaatttg aagaagatag
      841 caaacttata attgaagata tatatgattg tatatttaat agtataagtg agattgatag
      901 tgttgaaatt ttatataact ataatcttga aaaaataaac tttacatcaa gtgaaatcat
      961 aaattgtata tgtagagaat atgagtgtta tggtgaatac ttagaagaaa aagaaaagct
     1021 tatgaataca tttaaagaaa agattattca aatctttgag taataggtca atgctgaagt
     1081 ggcgctaatg cgccattttt caaatatata tgaattaagt aaaattattt tggaggaggc
     1141 gatttgtata gagaaaacag gtgtaatttt ggcaggggga agaaattcta gaatgggtag
     1201 agataaagcc tttttagagt tacatgataa gctttttatt gaaattgcaa tagaagcatt
     1261 taaaaacttt gatgagttga taataatatc taataatgaa gaattatatt ctaaatacga
     1321 tattaaagtt tataatgata tagtaaaaga tgttggtcca attggaggaa tatatactgc
     1381 tcttgaatat gcaaagtatg atatagttac aattgcatgt gatatgccat atctaaataa
     1441 tcaaatagtc gaaagaatag ctaataaaat gaatgataaa agtgtgattt cagtgacaaa
     1501 tggaaagtta caaccattgt gttctggata taaaaagagt ataataaata aagtatcttt
     1561 atgtataagg gaaaatgatt taaagctcag aagctttata gataaaatag ataaaagtta
     1621 tatatacttt gatgaagaag atttattttt aaatgtaaac actgttgatg agtatgaaaa
     1681 attaactaaa gtttaattag ctatggttgt aaaatagagt aggatttata tggaggagag
     1741 tttatgaatt tacttaaatg cgattcttgg gcagtaatat taaataatag cgataaagaa
     1801 agtaaagcat ataaaatatt agatgaactt aaaaggaata tgtacaaagt tgttgctata
     1861 gatgaagaga aaaaacctat tgaaggaata gatgtgtatg aatgtttaaa agatgttcca
     1921 cacaatattg atgttgtagc tattattgat aaacaatcta agatggatgt gattttagaa
     1981 gaagtagagc ttttggatat acaaaatatg tggtttgaaa aaggaagctt tagtgaaatg
     2041 atgattaaaa agactaaaga tttaaaatta aatatagaat ataatttaag tttatatgat
     2101 gagttgacta gataaaatta tatatcttaa taataaaaat ataattagtt aataaaacat
     2161 attattttat agtatagatt ggttaataaa agtacagatt ggttaatgaa acattaaatt
     2221 acaatatttg ttaaaaaaat tgtgagttac atgatataat gtattaaata ggatactaag
     2281 gagttgatat attatgttaa tagtaactac agaaaaagta gaaggtaaaa agatatcaaa
     2341 ggttttagga ttagtgagag gaagtacaat aagagcaaaa catgttggaa aagatatagg
     2401 agcaagtttt aagaatcttg ttggaggaga acttactgga tataatgaaa tgctcactga
     2461 agcaagacaa attgccatag gtagaatggt tgaagatgca gaagctaaag gtgcaaacgc
     2521 agtaatagca tttagactgt cttcagcttc agttatgcaa ggggcagcag aaatgcttgc
     2581 ttatggaaca gcagttgttt tagaagatga taatagtatt cttgaaaaat aaaatataaa
     2641 tagttaaaaa gaggcatctt ttaaaaattt tatggatgcc tttttttatt tgaaaaaact
     2701 aatttgagaa gaaattttaa ctattatgac aataaaatta gggtgatatt atataaataa
     2761 gtaaaattat attatataat gtatggtata gactttaaaa attttatttg tagagaaaaa
     2821 tatggacaat gttataataa ttatagaaat taaaagtact tacattcatt atgtatgtga
     2881 catgtataat tagttttata tttaatacag ttttgaagta atatcttcat tttgagaacg
     2941 ttggaataat aaaaaatgta ttattatagg tgatataaat aaaaaataaa tataatatgt
     3001 tatattgctg atagaaaaga gaataaaaga aaataggttt tattaaaaga gatttttatt
     3061 tataggactt aatcaatgta tagaggggtg agaacatatg tttaatgttg caattataac
     3121 acttagtgat aaaggatatg aaggaaaaag agaagatata acaggaaaaa agttaactga
     3181 atttgttgaa aatacaggag cttataaagt tactgaatat gtacttataa aagatgataa
     3241 ggaaatgcta aaggaaaata ttataagact ttgtaatagt aataaaatag atttaattct
     3301 tacaaatgga ggtactggtt tttcaaaaag agatataacc ccagaagcaa ctaaggaagt
     3361 gttggaaaaa gaaataccag gtctgagcga atatatgaga atgaaatcca cagaaattac
     3421 taaaaaggcc atactaagta ggggtgtaag tggaataaga aataattcta taataataaa
     3481 tttacctgga agtcctaaag gtgcagttga aaatctaagt tttataattg atgttttaga
     3541 ccatggcata gaagttttaa gaggagaagc tacagaatgt gccactaaaa aaagtgaata
     3601 gtatataatc tagatagata aaaaatagac aaagaggtag ataaagtatg attgataagt
     3661 atggaagaaa aatagactac cttcgtattt cacttacaga taagtgtaat ttaaggtgtg
     3721 tttattgtat gccaccagat gtaaaatttg ataaaaatta tataaatgaa aatctaagct
     3781 ttgaagacta caagtttatt ataaaggcta tggctgaaca aggtataaca aaaattagat
     3841 ttactggagg agaacctctt ttatatccta aattaaatga gcttataaaa tttacaaaag
     3901 aagaatgtgg aataaataac ataggaatga ccactaatgc tattgggctt tctgatagaa
     3961 tctttgagct tgaaaaatgt ggcttaaata aagttaatat aagtttagat tctttgaagg
     4021 aatataagta taaatccgta acaagaggag gaaacttaaa agatgtctta aaatcgattg
     4081 aatcttgttt aaacttaggt ataaaagtaa agttaaattg cgttgctata agtgggttta
     4141 atgatgatga gcttaaagat tttatgtttt taacagttaa atcaccaatt gatgtaagat
     4201 ttatagagct tatgccatta ggtgaggcta atagagttta taagaagggt tattttaatg
     4261 taaaagaaat gattgaaaaa atatatggtt tatataaagt caaaaatgat gaaaaaagta
     4321 cagcagaata ttatatgttt aaaggagcta aaggaagaat aggaattata actccattaa
     4381 gctgttcatt ttgtaacact tgtaatcgta ttagacttac ttctagtggt gcaataaaat
     4441 tatgtctaca ttcaaaagaa gaaatagata taaaaccatt tttacataaa ccattgttat
     4501 ttagagaatc tatgaaagat ataataaaac aaaaaccaga aaaacatcat ttaatagaaa
     4561 ataaatgtag tgatacaaat aggcgtatgt atgaaattgg aggataagta atgttaacac
     4621 atataaatga aaaagggtat gccaagatgg tggacataag tgaaaaagat gatactaaaa
     4681 gaacggccgt agctacagct acaattactc ttagtaagga agcattagaa aaagtaaaaa
     4741 atggtcagat aaaaaaagga gacgtattgt ctgtagctca agttgcaggt ataatgggag
     4801 ctaaaaatac atcttcaaac ataccaatgt gccatcagat taaattagtt ggatgtgatt
     4861 tagaatttga tgtagatgat tctatgtctg ctattaagat atacgcaacc tgtaaatctc
     4921 ttgggaaaac tggagtagaa atggaagctt taaatgcatg ttctttagca gcacttacaa
     4981 tttatgatat gtgtaaagca gttgataaaa aaatggttat aagtaatatc catcttgtag
     5041 agaagactgg tggaaaatca ggagatttta agttcgagta aattaataat atattgggtt
     5101 aaaatttgga ggaaatatgg cgaaggtagt gtcaataaat ataagtgaga aaaaaggtgt
     5161 aattaaagtt cctgtacaat gtgcagagct aaaaataaat catgggatag ttggagatgc
     5221 acatgcagga aactggcatc gtcagataag cttacttgga aaagaaagca tagataaaat
     5281 gaaaatgaaa ggctttgacc aattaaaata tggagatttt gctgaaaatg taactacaga
     5341 agggatagaa gttttttccc tgcctgttgg tacaaagtta agaatagggc aatgtgaagt
     5401 tgaagtaact caaataggga aaaaatgtca caatggatgc gaaattaaga aattgacagg
     5461 tgactgtgtg atgccacgag aaggaatttt tgtaaaggtt ctaaaagaag gaattataaa
     5521 tgtgaatgat tcaatagatg taatctaatg aagtcactat t
//
LOCUS       DACULQ010000209         5495 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.211, whole genome shotgun sequence.
ACCESSION   DACULQ010000209 DACULQ010000000
VERSION     DACULQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.211"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            281..1579
                     /locus_tag="JA154_16625"
     CDS             281..1579
                     /locus_tag="JA154_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAT7997456.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGYPLPIPYIMPLKETLCMS
                     MSRGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            1769..3160
                     /locus_tag="JA154_16630"
     CDS             1769..3160
                     /locus_tag="JA154_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997457.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            3337..3924
                     /locus_tag="JA154_16635"
     CDS             3337..3924
                     /locus_tag="JA154_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7997458.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYSNSKSVSPYLLLPLSYIEFAQEETHLFKL
                     LFINDLDLMMEEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            3957..4748
                     /locus_tag="JA154_16640"
     CDS             3957..4748
                     /locus_tag="JA154_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7997459.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(4929..>5495)
                     /locus_tag="JA154_16645"
     CDS             complement(4929..>5495)
                     /locus_tag="JA154_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997460.1"
                     /translation="LTPYNTNLDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDD
                     YRMITRAFQYIIIASYIFVGLISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQL
                     RNMLYREGLNISIKAICTSSTLGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFV
                     LIGIQVLVTELLVRNIEKKSLTERLRSQ"
BASE COUNT         2025 a          623 c          949 g         1898 t
ORIGIN      
        1 tttatattaa aaaccattct ttaaatgagt ggttttttat tttgaatata tatattctaa
       61 acctaatagc attcctatct ttaattagcc aaaaatcaac tggatttcat atagaccata
      121 attttaaatt actaaaaaag acaatatgtt gacaaagatg aaatatgttg ataaaatgaa
      181 atagtcaata tggtgtctta atttaaaaat ttatttatct tgtattagat aaagctgttt
      241 tatataaaac ataaataatt attataagga gaaatagatt atgaaagtaa atgtatacaa
      301 tgtagaatca tttgtggatg aaagacttaa gattatggtt tcagacttaa ttccaaatac
      361 taaacttaaa ataagtgtga aaatggaatt tccatggtgt aagggagaag aattttcatc
      421 ttatggagtg ttttgttcaa atgaaaaagg agaagtagat ttagatttag ttgaacctat
      481 agaaggtact tataaggctg ataattgtat ggggttaatt tattcgctgg aaaaatctaa
      541 aactgaaggt aaaaattttg cagaaaatat atctattgat aaaccaataa ttatgaatat
      601 gatatttgaa acttctattg aaagaaaaga agttgaatta aagagaatat ttaaatcaga
      661 agatattacc acaaaataca tatctgatga gtttatagga aaattatttt ataaacaaaa
      721 ttctaataat aaaacaattt taatgctagg tggttctgat ggaaatttag acgctctaga
      781 ccttttagca gctccacttg catctagagg attcaatgta ctaacagttg catactttgc
      841 tttagaaggg ttaccagata aattagaaga ggttccatta gaatattttg aaaaagtatt
      901 tgagtggatt agtgaaaatg aaattaccag tactaaagaa atatttgtac atgggacatc
      961 aaagggtgga gaattggcct ttttacttgc ttcaagatat gaacaaatta aaaaggttgt
     1021 agtatcacaa cctcacattt attgtttcca agctcttaat ggattgatga gtggaaatga
     1081 tacttcatca tggtcatata aaggagagcc atttccatat atcaaagtag acaatgatat
     1141 attctttgaa gaacagaaaa aaaatatatc aaaagatatt ccatttggat ttaatagtac
     1201 ttataagaaa agtcttaaaa gagcagagaa tagaaaagag gctagaataa aaatagaaaa
     1261 ttccaaagca gatatactta tgattgctgg aaaagaagat aacatatgga atagttatga
     1321 agcttgttta gaggcattag aaatctttga agaaaataat tatccatata acgtagaatt
     1381 attaacatac aataatatgg gttatccgtt accaattcca tatattatgc ctctaaagga
     1441 gactttatgt atgagtatga gtagagggat tttttcttca ggtggaactg tagaaggaaa
     1501 ttcaaaagga cagtttgaat catggaatag aactattgaa ttttataaaa aacctttatc
     1561 aagtgattta aataactaat attaagtgat ttaagtaact aaaaatgaag cggtctttct
     1621 atatatgcac aaaatctatc aattttatag atttaattta ttgtttatat aatttgcatg
     1681 ttatatacta attttaatga cattgataat ttataaacaa ttataaagtt gtaaacttat
     1741 aagagttatt gaaaggtgga gtgaagttat ggaaagactc gctagaaggg aacaacctca
     1801 tataaaattt ggaatgttta agataagaat tccatttata cattacagat ttgagaaacc
     1861 agaagctatt caaggtataa tatcaagtat gacaagttta ggcacaattg gccttagtac
     1921 acaaattttg ggattaccct atgaagtggc atggagtatg gctatcatta actcaatatt
     1981 atactgtttg catgttttta tgggagaccc agttgtacct ggctggatta cagcttctat
     2041 aactcttaca actgcatatt tgttaaagtt cagcatgggc attgaaagaa tacaagcatt
     2101 aacagcttta caaatagatt taggaataat atttatttta atgggtataa ctggtgtagc
     2161 tggaaagtta gttagtaaga taccaaattc tattaaaggt ggaatactaa tgggtgtcgg
     2221 tatatcgact ataatatcag agtttaatcc taaagggaga tttgattctt atcctatatc
     2281 tataacagta ggaattttgg ttgcttgttt tgtaatgttt tctgaaagat ttgatactct
     2341 aaaagttaag aataaatttt tatttcattt aggagaatat ggtgtagtat cagctatttt
     2401 agtgagttta atagtaggta tatttagtaa agaaatagaa attcctagat ttagttttga
     2461 caatttagtt tacatagtag attttaaaaa tctgataaat acagtatctc cttttggaat
     2521 aggttttcca agtgtaatgt tatttataca agggatacca atggctttta tgatatacat
     2581 aattgccttt ggtgatttta taacaggtga aaacttagta ctttctgaaa gtgaaaatag
     2641 aaaagatgaa tatatagatt tcaattcaaa taggtctaat gttataagtg ggataagaaa
     2701 tatttttatg gcaatctttt ctccatatat accaatgtgc ggtccattag ctgcgacact
     2761 tacaggaagt gttgctcaaa ggtacaaggt tggaaaggaa gccatgcagt ctgtttttag
     2821 tggcatgggt acattagtat gggtttcagc aatttttatc tgtttttatc caattgcgca
     2881 gattgctaca cctcttatac cattagcatt gtcagtaaca cttttagttc aaggatattt
     2941 atgtacaaag ctatcaatgg agctatgtaa tacaggtgtt gagaagggat tgattggact
     3001 tatgggaggt gtaattgctg ctaaaggcgg aacatgggga cttgctgtag gttttatact
     3061 gtatttcatt ttaattgatt ctaaaaaaag aaaagaaaaa gatattaatt caatagaaga
     3121 ttgtgtatat gaaatattag acaaaaagaa gagtatttaa aggaaaatct gagttgctgt
     3181 tatctattaa tagattaatt gcaactcatg ttttatttat aagttttagg aattgcaaaa
     3241 aaatgcaaag ctttttcttg acataatttc ttgatatgtt actatagtta tataacatat
     3301 gtgttttata aaaatttaat ggagaggtga acatatatgc cacctaaagt aaagataaca
     3361 aaagaaatga ttttgaatac agtcttggat attacaagag aagtgggatt tgaagctgta
     3421 aatgcgagga gtattgcaag taaattacag tgttccactc gaccaatttt tacttgttac
     3481 gaaaacatga atgaattaaa aaatgaattt cttgcttttg catatgaata ctataatcag
     3541 tatgttgtta attatagtaa ttctaaaagt gtaagtcctt atttactgct tccgctttca
     3601 tacattgaat ttgctcaaga agaaactcat ttatttaagt tgttatttat aaatgattta
     3661 gatttgatga tggaagaggc aaatgatttt tataaagaaa ttgacaatga aaaaaaggca
     3721 agactttttt cggaaagcat tggaatagag ttggaatgtg caaaagtaat atttttagat
     3781 ttatttcttt atactcatgg catagctgtc ttaacagcaa caaaaaaatt aacattagat
     3841 agatatagtg ctgaaaaaat gttgaaaaat atattaacag cttttataag acaagaaaaa
     3901 ccagattgga atttgtctat ttgatgttta ttaactaata tagaagaggt gaaattatgg
     3961 aaacaaaaca atacttaatt gacttttata atacttatga tgaagatagt cgacttgcat
     4021 taaaacatgg aatggtggaa tttctcacta ctatgcatta cattgataag tacataaaat
     4081 caggtgattg tgtgcttgaa attggcgctg caactggtcg ttattctcac acactggcac
     4141 gtcagggata tgatgttgac gcagtggaat tagtcgaaca taatatagag gttttccata
     4201 aaaatactca atcaaatgaa aatataagca ttactcaggg taatgctatg gatttatctg
     4261 ttttcccaga taataaatat gatattacgt tactgttagg accactatat catctttata
     4321 acaaagaaga taaacaacaa gctttacatg aggcaattcg tgtaacaaaa cctggtggtg
     4381 tggtttttgc tgcatatgtt atatctgatg gctgtctcat tgacgagggg ttccatagag
     4441 gaaatattaa tgtatctgaa tatattgaaa aaggtttgat tgatccacag acatttgctg
     4501 ctaagtctga accaaaggat ttgtttgagc ttgtacgtaa agaaaacatt gatgatttga
     4561 tgtctgcatt taatgtaact agactgcatt atgtcgcatc agatggtttg gctttatata
     4621 tgcgtgaagc agtagactct atggatgatg atgcatttgc attatattta aaatatcacc
     4681 ttgctacttg tgaacgtgaa gatttagtag gggttacaag tcatgcaatt gacatattta
     4741 gaaaataagg aatatgcttg tttatgtctc tggtgatgtt gctcggagat ataagtaagt
     4801 gtatctgtat aaaataaaaa caagtatagg gatagaaaat tatgtaaagt caaaattttc
     4861 tatccctata gcttttaaaa aatcatatta aaacttgtat aaaaaatggc tattttttat
     4921 atacattctt actgagaacg taagcgttct gtaagtgatt ttttttctat atttcttaca
     4981 agtaattcag tcactaaaac ttgtatacca attaatacga atgaaaaaat taaaatagta
     5041 aatatagaaa atttaaaatt aataaagtct aatctaatta catcttttat aaaagtgcaa
     5101 agtagatttg aaccaaaata acctagtgta cttgaagtac atatagcttt gatacttatg
     5161 tttagacctt ctctataaag catatttcgc agttgctttc ttgtcattcc aacggcttga
     5221 agtaatgcaa actctttttt tctaattaaa acgctgttta ttagggtatt ggtcatattt
     5281 agtataccaa agcaagaaat taaaccaaca aaaatgtatg aagctataat tatatattga
     5341 aatgctctag taatcattct ataatcatct tcatatgaat aaagcattaa atttgaattt
     5401 ttctgaatta tacttaacaa cttttcttca acttctttag aatgcttttt atctattaaa
     5461 atagaaaaat ctaaatttgt attatatggt gttag
//
LOCUS       DACULQ010000210         5293 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.212, whole genome shotgun sequence.
ACCESSION   DACULQ010000210 DACULQ010000000
VERSION     DACULQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.212"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(160..951)
                     /locus_tag="JA154_16650"
     CDS             complement(160..951)
                     /locus_tag="JA154_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT7997461.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(987..1643)
                     /locus_tag="JA154_16655"
     CDS             complement(987..1643)
                     /locus_tag="JA154_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HAT7997462.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(1636..2601)
                     /locus_tag="JA154_16660"
     CDS             complement(1636..2601)
                     /locus_tag="JA154_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HAT7997463.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKNLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(2707..2813)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3103..4047)
                     /locus_tag="JA154_16665"
     CDS             complement(3103..4047)
                     /locus_tag="JA154_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HAT7997464.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSASIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLVKSKVLELYNHHCI"
     gene            4158..4625
                     /locus_tag="JA154_16670"
     CDS             4158..4625
                     /locus_tag="JA154_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997465.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(4795..>5293)
                     /locus_tag="JA154_16675"
     CDS             complement(4795..>5293)
                     /locus_tag="JA154_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997466.1"
                     /translation="KNPNESVNNNEGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQF
                     KDKEYPGNEKYLSDVKAAYKDSRDKIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERL
                     VKDIDKRIEKLDKVSDEDMKKGQNDFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGIT
                     GNNKK"
BASE COUNT         1694 a          907 c          579 g         2113 t
ORIGIN      
        1 tttagattcc atattcccca ccacttcttc atttttatat agttttacct ataaatatgt
       61 taccacaatt cttccaaaaa atttgtcact ttctagttat aaaaaaaaat gagtaaatat
      121 ttttaatttt actcattttg tagattttgt atattcagtt taaaaagctg gaactatact
      181 tcctttatat tcatcttgta taaatttttt aacttcttta ctattcatag cctctgataa
      241 agccttaatc ttatcactat ctttattatt ttctctacaa gctattatat ttacatatgg
      301 agaatcactt gattctatta ctattgcatc cttagtagga tttaagttag ctgttaaagc
      361 ataattagaa tttattactg ctccgtcaac atcttttaat actgttggta attgctctgc
      421 attcatttct tttattacta tatttttagg atttttagtt atatcttttt tagttataag
      481 ctctccatct tttacttcta tcaatttatt ttttgctaat aatttaagtg ctctagctcc
      541 attagttgca tcatttggca cagctataac agctccatct tttaattcat ctaatgactt
      601 aactttttct gaatagaatc ccattggctc tatatgaact ttagatgtat aagtaagttt
      661 atatcctttc tccttcacag tttcctctaa aaatggaata tgttgaaaga agttagcatc
      721 caaagaccct tcatctaaag ctgtatttgg aagtacataa tcatcaaata tttttacttc
      781 taaatcatat cctttttctt ttaacaaagg ttttgcaact tccaatatct ttgcatgtgg
      841 atttgatgaa gctcctacta gtattttttt atcctctttg tttgaacaac ctactgctga
      901 tattgctatt gctgacacca atgctactga taataatttc tttaatttca tttttactcc
      961 ccctctttaa aatgtttata attaatttat tttagtttct tataagccag atttcctact
     1021 ccctggatta tctgaactaa tattataagt gctattacag tatataccat tactgctgta
     1081 tcaaatcttt gatatccata aataagtgct atattaccta atcctccagc tcctactgca
     1141 cctgccatag ctgtataacc aagtatagat attattgata atgtaattcc agatactatt
     1201 gaaggcatag cttcctttat cataacttta aatactattt gtctttttgt agctccaaaa
     1261 gattttgctg cttctatcaa tcctttatca acttcattta atgagctttc tataattcta
     1321 gcgataaacg gtgctgcccc aatagttatt ggcactattg cagcagtttc ccctatactt
     1381 gttccaacga ttaatctagt tattggaatc attgcaacta taagtatcat aaatgggaaa
     1441 cttctaaata tatttactat aaatcctaat gcactattta ttgtgctatt tggttttaat
     1501 ccatctggtt ttgtaactac taatatgatt gctaatataa atcctaatat tgttgccact
     1561 atagttggta ctataaccat atacaaagtt tgtaacaatg catttggaaa tagtgttgtt
     1621 aaaaagtcaa ttaaactatt catttatcat ttcctcccat cttatgccct tactagttag
     1681 atattgtttt acttgctctc cacttttatc tgaaatgttt ataataagtg aacctaaaat
     1741 atcatcttta aattgttcta atttcccaaa tattatagac acatctatat ttagttctct
     1801 tgccatagtc gtaataatag cttcattaga aatatcttta gggaatagta tctttatatt
     1861 tgttcctttt ggcaatatta tactttcttc acctattaat tttctaagtc ctttagtatt
     1921 tcttaagaat atttcttctg tatctcctac ctctaatact tctccatttt ccattattgc
     1981 tactcttcca caaatctgct ttataacttc catttgatga gttacaacta ttattgttat
     2041 tcccaaattt ttatttatat cttcaagcaa tgaaagaatt gactttgtag tatttgggtc
     2101 taatgctgaa gttgcttcat cacaaagaag tacttgtgga tttagtgcta aagctctagc
     2161 aattgcaacc ctttgttttt gtccgccact tagatttctg ggtttatcat ttttcttttc
     2221 agatattcca actacttcta ataactctaa aactcttttt ttaatttctg cctttgagta
     2281 tccaaaacat tctaatggca atgctacatt atcaaataca gtttttcttt caagcaatga
     2341 aaagtgttga aaaatcattc ctaactcttt tcttaaatct ctcatttgct tttcattcaa
     2401 tgactttact tctttatcag aaacaagtac actgccttct tggtattctt ctaatccgtt
     2461 tatacatctc agtagtgttg atttccctgc accactatga cctattattc caaaaatttc
     2521 tccagattct atttcaatac ttacatcttt aagcacttga atctttccat aatacttatt
     2581 aacatttttt atgctaatca taaaatttcc tcccatatat tctgctcagt ttttatgttt
     2641 atcctctctt atgtattaca acaaaataaa agcccgagta taaactcagg cttagaaagc
     2701 tatttatact catctatcaa ctatattagt tgcaggattt agcaccaatg tatataaaat
     2761 atactggttg ccgggtttca aagggccagt ccctccacca ctcttgataa gacgttattt
     2821 aatttaattc tattatcatt tgttgctatg ttatatatga tatattcatt aatagtattt
     2881 gtcaataaat ttatttaaat tttttatttt ttattatcgt taattaacta tttttaatta
     2941 gttttctagc tcctaacact ttatatttta atgtctatat aactatagaa catcattata
     3001 agaaataaat taaataacat caatctatct aaatcatctg taaagtaaat taagaaaatt
     3061 aacattttat ccaaactcta tcctactatt aaataaaata atttaaatac aatgatgatt
     3121 atatagctct agtactttag actttaccaa atgtttaagt tctataggtt ctattattgt
     3181 agcatttttc ccaaaagtta aaaaatattc agccacccag ttaagtgtag attttggtat
     3241 atacatatct atgtatccac ctttagacaa gattttcaac cctcttgcca acaaaaattc
     3301 tgtttcacaa cgttttactc cctcatctgt caaattaatt ttaatatgat aatctgtacc
     3361 tacttccata ttcttaagat aatcatgtat agaagataaa accttatatt tcttacttga
     3421 taagttttct atactcaaaa tcttcacaat tctgtctaca cgaaattcct taatagatgc
     3481 tgattttata cagtaagctg gacaatacca aagtccattc atggcataca aaccaatagg
     3541 aacaatggta cgtacactat ttttttgctt tgatgaatat tcaattttaa ttgtagattc
     3601 attcattact atattaaaca attctttcaa taatggtgtt gaacagtgcc tatctggtgt
     3661 ccaaaaaaca aatttacgcc ttatattctc aatattatat tgaatatcat ttggtataca
     3721 atttaaaaat ttttttaaaa cagaaatagt ttcttgctca aatggcaaat cattataata
     3781 ttctagtgac tgatatgcaa agaatataga ttttacctca ctttctgaaa atgcaatcgg
     3841 tggaagtata cgctctttaa gtacatgata tccaccagcc gctccaactt ttgaatataa
     3901 tggaaatcca atactctcca gcacctgtaa gtctcttaat atagtacgtt ttgatacaga
     3961 aaattcttct gctaattctc ctgctgtaaa acttcttttt gcatttattg ttatcatcag
     4021 ttcaattaaa cgctcagctc ttgacatatt tatactccta atttaaatat agtgacaata
     4081 attgtcacta ttatatgata agatattctt acatttaaag caataataat agtaaagaaa
     4141 gaagggactg atactttatg gaaaataata ctattaaggc tgatgatatt atcaattatt
     4201 gtctatcaaa cttagatgat gttttattaa tgaatagttg gggggaacga gcaatttact
     4261 acaatcctaa tggtatttta aagcgaggag tatatgttct taccattaaa gaaaaagaca
     4321 gtaatcatga taaaggttcg ttggttagtc gcccaaatgt ataccgtgtg aatataggat
     4381 taaaaaaaga aacttttatt gaaatgtttg gatatattcc aaaacgtcca ggtgtaggtc
     4441 aaatagttga tatggatttt gattttacaa aattggacac aatcatgcca catcctatct
     4501 actcatggat gggatggata tgcgtcctaa gccctactga aaagacattt gagaacttta
     4561 aaactttaat aggagaatct tacaattttg caaaacaaaa atttaaaaag agaaaaaaca
     4621 agtaaaaacg tatgaaaata atccctaaat aaattataat aaaaatatta aggttacaag
     4681 ataaaaatta atatcttaat aacatgcaaa aaagcgaagc caaaaaatgt attatttata
     4741 aggcttcgct atatctcaaa taatttattt caaatttcaa aactatttat tctattattt
     4801 cttattattt cctgttattc ctaatctatc attcatatct tttatcattt ttctaaaacc
     4861 actacctata tcatttccaa catcttcagt tccatgaact aattttataa agtcattttg
     4921 accttttttc atatcttcat cagatacctt atctaatttt tctattctct tatctatgtc
     4981 ttttactaat ctttcccctt cagcgattag gtcatcattc attttcttta actctgtatc
     5041 ttctgtttta ccatcttttt taagagatgt tacaaatgat tgtattttat ctctactatc
     5101 tttatatgca gcctttacat cacttagata tttttcattt cctggatatt ctttatcttt
     5161 aaactgatta tttatacttt ctggagtatc ataaatagaa tatctattta gacctgctat
     5221 ataatcatta tagcttgttg aataataatt catatatcct tcattattat ttacagattc
     5281 attaggattt ttt
//
LOCUS       DACULQ010000211         5290 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.213, whole genome shotgun sequence.
ACCESSION   DACULQ010000211 DACULQ010000000
VERSION     DACULQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.213"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            14..214
                     /locus_tag="JA154_16680"
     CDS             14..214
                     /locus_tag="JA154_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997467.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(421..1011)
                     /gene="spoVFB"
                     /locus_tag="JA154_16685"
                     /gene_synonym="dpaB"
     CDS             complement(421..1011)
                     /gene="spoVFB"
                     /locus_tag="JA154_16685"
                     /gene_synonym="dpaB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089485.1"
                     /note="involved in production of dipicolinic acid
                     (pyridine-2,6-dicarboxylic acid, DPA) which is synthesized
                     late in sporulation in the mother cell and accumulates in
                     the spore; mutations in this gene result in a lack of DPA
                     synthesis; presumably functions with SpoVFA to form the
                     synthase enzyme; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HAT7997468.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(1014..1889)
                     /locus_tag="JA154_16690"
     CDS             complement(1014..1889)
                     /locus_tag="JA154_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HAT7997469.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(1965..3356)
                     /locus_tag="JA154_16695"
     CDS             complement(1965..3356)
                     /locus_tag="JA154_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HAT7997470.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRIIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDILNIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(3360..4496)
                     /locus_tag="JA154_16700"
     CDS             complement(3360..4496)
                     /locus_tag="JA154_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HAT7997471.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(4528..5073)
                     /locus_tag="JA154_16705"
     CDS             complement(4528..5073)
                     /locus_tag="JA154_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAT7997472.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
BASE COUNT         1770 a          875 c          622 g         2023 t
ORIGIN      
        1 aaggaggata agcatggaaa aattaaaaac aattagcgtt ttttctttga ttataagtgt
       61 aattttaacg attggaggta ttggaatagt aacttattat gtagataatt tgtttattag
      121 aggactatca gtatttgttt taattatgtc ctcttctttt gtatcaacaa cagttagatt
      181 gatatttgaa gagtcaaaac gatataaatt ctaatttaaa aattggatta agtaaataat
      241 tttaatatag caaaaattac ggagattagc tcaaaataga ctttttagtc catgaggcat
      301 ttttttgtat gaaattaaat atattttcat catttcaaca agaaaaagag gcttctctct
      361 attttgagac agcctctttg ctagtattgt atctaataca taatattata agaaccaatt
      421 ctattgatgt ggacttttta taacaggctg tagctgtttg ttctctagag caagttctat
      481 agttggaatt attagctcag tatgtgcaat catagaatta ggctttgctt tgcaattatc
      541 ttgaccaaaa ggtacaaaat atatattttt agaattaagg agaatgccaa tatttttgaa
      601 attaaagctt agagcatcat tcgttgatat tgatattact agtggtttat cattacgaag
      661 atgacctttt gctgccatta atactggtga atctgtaata ccatttgcta attttgcagc
      721 agtatttcca gtacaaggag ctattattat aatatcagca attcctttag gtccaaatgg
      781 ctctgcctct tcaatcgtaa ctataggttt atttcctgta agttcatatg cttttttcat
      841 aaactcttca gaatttccaa atctacaatc gatattttgt gatacatctg aaaatatagt
      901 gtgtacatta gctccttctt taaccaaatt ctctaactct ataaatattt tatcataagt
      961 acaaaacgaa ccagtaaatc ctactccaat tgtaagtcct tcaagtctca tttctaatcc
     1021 cttctttcat ttataatatt ttctatagcg ttaacaagaa tttcaccaga tgtctttggt
     1081 gaatatttac caggaagccc aaggcaaaga gtcgcattaa tccctgcttc tttaacaaca
     1141 gaataatcta ctcctccagg agaagaagct atatctataa tagttacttc ttgtgatact
     1201 ttttctaacc tttctttcgt taaaatacac tctggtatgg tgttaaaaat ataatcaaat
     1261 tgtaaaagtt tttcatcaag ttgacttaaa gaaatactgg aatatccaaa tgcattagcc
     1321 gtacttcttg catcattatt tcttgcgcca acacatacat ttgcatccaa agcgtataat
     1381 ttttttgcaa gtatttgtgc acatcttcca taacctaata ctaaacagtt actttgatgt
     1441 atattgatta tacttctttt aatagcttct gctatagccc cttcagctgt agcaactgca
     1501 tttaaaatag caacatcatt cattttcata aaatcataaa tctgaataga gtgttgctcg
     1561 caaaatttac aaactttagt tggtatgtta cctccaaata atatttgatt aggagataaa
     1621 ttaattagca agttatcaat agtagcatca agacaatcat ccgtattttc aatattaact
     1681 tgattctttg taaatggaat tggacataaa ataatggaac ttttggatag tgcttcagct
     1741 agtgaagttg attttccaac gattccattt ataatttcat ttgatatccc ataagtgact
     1801 acagaatacc ctttattaat tagttttttt accatgtaca cttgtcgcag gtctccccca
     1861 ataacagtta tatcgtattg atttgacata ttgtaagcct cctttactat atacaatatg
     1921 tttttatcaa tacattggtt cactaaaatt actaaatatt atacttataa catttttaat
     1981 aatgctaatc tatctatctt ttcagaagag gtatatggaa atgaatttaa aaagataatt
     2041 ttctttggga tttcattttt cattagatta tctttcattt taatcaacaa atctttcttc
     2101 gtaattttgt ctatagttat tacaaataat acaatttgat ttcctcttat agtgtctgag
     2161 tacggcaata caattgcatc ttcaatttga ggaatttttt taacttcatt ttcaatctta
     2221 gtagaactaa ctttaaatcc tccaatattt aaaatgtcat cacttcttcc ttcaaatatt
     2281 aagtatccat cattatcata atatccaata tcattgacag aataaggttt ggttgcatta
     2341 tatactgcat attctgtatt cacaaaaatt tttccttctc taatatatat atctattcct
     2401 ggaaatggtc tgcctacact tagaggtttt tcaattagtt catcatagca taaatatgta
     2461 atataattta attcacttgc cccatagtat aaaataattt cagactttgg catatatttt
     2521 tttagatttc ttgcagtatc ttcaaataat aattgagacc ctgtaaaaat acttacaact
     2581 ttgagtactg gttcttttaa gtgtttaacc aaaagttgaa gttttgtagg tataaggtat
     2641 atgttagtta cattatattg ttttattatc cttatccatg atttacaatt aagacctgaa
     2701 cttattacaa tactacctcc ttcatataat actgacatta tagaatttag attccctgta
     2761 aaactaagtg ttccattaac aaacaagatt gattccccag agatattaaa aataccattt
     2821 tgtataggaa aaaatccagc ccaactctca tatgttctat ataatacctt tggaacactt
     2881 gtagaacctg aagataaagc tcccatacaa atacttttat ctaaataatt tataaggttc
     2941 ttatattgat acatatatat attaaattta ggattaaaaa tagaaaaatt aacttttttt
     3001 gctacttcat tattgttatc atcatataaa tcaataacgt ccatagattc atcagatata
     3061 atgattgaaa tattattttt taataaaata tcatctaata cttttttaga aagattataa
     3121 tgacaaataa taggaatatt ttttgaataa ttaatagcaa aaaaactaat caactgaaac
     3181 attatatttt tagaatatat taaaattggg atattttcac ctaaagaaat ttgttttcct
     3241 agttcctcac tataactcaa tatattttca taagtatatt tttctccatc tataattaaa
     3301 aaatttttat ttttatgcct ctcttttttt attcttaaaa gttcataata gttcataatc
     3361 tacacccttt caagcagtaa agctgaacct agaccaccag ctgcagctat agagcaaatt
     3421 ccatatctac ccttagtctt ttctaatgct tttaaaagat gtaatgcaat aattgcacct
     3481 gaagcaccat atggatgtcc atacgctaaa gctccaccaa atatattata tctatctatt
     3541 aactctggat attttctttg aaacaacaca tctataactg caaatgcttc attaaattca
     3601 acagcagata catctaaata attaagactt tctatctcta aaagtttatc cattgcttgt
     3661 atcgctgatg ttggacttaa ggttgcatct gttcctattg tgcatgtatt aataattttt
     3721 gctttaggct tcttttttac atgttctaaa tacttcttag aacatattat aataaatgaa
     3781 gctccatcat taataagaca tgaatttgct gcatttgtaa tagtttcttt tcctaaaatt
     3841 gatggcatcc tatctaataa cctttgactc atcttatcac gtataccttc atcataggta
     3901 ctattattta taggagatat aatatcttca agtatttttt cctctcttgc ttcttttgct
     3961 cttttatgac tttcctttac ccaaaaatcc aagtctgctt tctcaatttg atataactct
     4021 gctactcttt ccgcaccttc gagcatacta ttttggctat tatcatcagg agaaaattga
     4081 gcaactgtat aatttggatt atttgtatta tatcgcttat cattcttatg atatgtgcgc
     4141 attggttgta gagaactact ttcaaaacca cctgcaataa ttaaatcaca ctgaccagat
     4201 ttaacctttg aaaaagctat gtctatactc atcatagcag aagcacactg catatccaca
     4261 gtaaaagcag gtacttcatt tgacactcct gctgttaaag tcattaatct agctatattc
     4321 cctcctgtcc caactgcatt tccacatata atctcatcaa ttttatcaat ttcatacttt
     4381 ttaattaaat cttttaaaac acttgctcct agtaattcag gttgaacaaa ttgaaaaatt
     4441 ccatttttta gacctatatg acttctcaat cctcctaata taaaaacttc cttcatacat
     4501 tacacccttc cttatattat agatttatca tagatttctt ctgagtacat tgttcagttt
     4561 agtgcctaaa aaagatgcaa taactgactt tattgtatct ccaattaaaa atggtactac
     4621 tgctgcaaaa agtgctgctt ctactgtcat ttgattaaaa tagcacataa atactgttcc
     4681 cataaaatat ataataggca ttccaacaaa aattgttatt agtatatatc tcttaatatc
     4741 attatttcgt ccttttaata aactaataat tagtgctgca aacaaaaacc caaaataaaa
     4801 accacctgtt gggcttaata actttccaat accagatgta cctccaccaa atactggaat
     4861 cccaattaaa cctatcatta tatatacacc aactgtacta aatgcttgtc ttggtgttaa
     4921 aatcaaagct attaaattta tcattattgt ttgtgctgtt actcctgctg gagtaaatgg
     4981 caatggtata tatatgtaag atgatataca caataatgct acacatattg acattttagt
     5041 taaatcttga atatttactt tagtattttt catttttata attcccttca tatgtcttat
     5101 ttcatttagt ttatatgtac atatacaaag cttctattaa agctttgttt tagtataaaa
     5161 taaagacgtt tttttgtcaa ctattataag catttaggtt tacatttaat ttataagtaa
     5221 actttatact gttactttat attttaatag tcatttataa aactttgctt aaaaacagct
     5281 ctatttagac
//
LOCUS       DACULQ010000212         5287 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.214, whole genome shotgun sequence.
ACCESSION   DACULQ010000212 DACULQ010000000
VERSION     DACULQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.214"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(437..1843)
                     /locus_tag="JA154_16710"
     CDS             complement(437..1843)
                     /locus_tag="JA154_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7997473.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVEFDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(1888..2601)
                     /locus_tag="JA154_16715"
     CDS             complement(1888..2601)
                     /locus_tag="JA154_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7997474.1"
                     /translation="MIMESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEE
                     AVRVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLG
                     ADDYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQE
                     ISLTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTP
                     STPKHLITVRGLGYKLMIN"
     gene            2766..3680
                     /locus_tag="JA154_16720"
     CDS             2766..3680
                     /locus_tag="JA154_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacitracin ABC transporter ATP-binding protein"
                     /protein_id="HAT7997475.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QKLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3680..4441
                     /locus_tag="JA154_16725"
     CDS             3680..4441
                     /locus_tag="JA154_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease"
                     /protein_id="HAT7997476.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMFMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4453..5190
                     /locus_tag="JA154_16730"
     CDS             4453..5190
                     /locus_tag="JA154_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease"
                     /protein_id="HAT7997477.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1822 a          814 c          691 g         1960 t
ORIGIN      
        1 ctataataat atacttacaa tagttacata gaagctatta taactacaca gaacagcgta
       61 taattaaaac ttttttagga tgtcattata ttttttctta aaattaaaaa acactatacc
      121 ataattctat tttagtagaa tttttttgtc aataataaaa ttaaatatta actaaaaaat
      181 aaaaaaaatg gaattatcta aagaatttaa taaccttaca ataattccaa aatattattt
      241 ctatgaaaat tttgatacca tgagtaactc agccataagc gcataaagca caagttactc
      301 tttactttaa taaaacttga caagaaaaga atttatcctt attctactct aatagagtta
      361 atttgtcaat aaagaataaa attattttca aaatattttc attcttcatt cttatctgtt
      421 attattttat aaaatatcac ttaattagag gtaattttag tattgttttt agtccctgac
      481 tatcaatgct cgtaatatct aatttacctc tatgtgcatt aataatgcca gatactatca
      541 aaatacctag accatgattt tgacctttgg catatgcctc acttagtcca ttttggctct
      601 ttaataaact attaattctt tcttgactca taccttttcc atcatcttca acaattatat
      661 agcaaaaatc taaatcagac ttagaggtaa cccttatatt acatccttgt tggttatgag
      721 ttatactatt ttgtattaaa ttactaatag ccctttttaa taatttttta tctgcatcta
      781 taataatatc ttcatttagt atatcaaatt caacgtaaaa gtcattatcc aaattattat
      841 ttataaactc agaaataagc tctctcaaaa acgcagaaac tctaatctta tcacaattca
      901 atgcttgcat attatattca agtttagaca ctaaatttag gtcatttact aattctctga
      961 ttttaatccc ttgattgcat ataatagaca cttgctcttg ttctctattt gaaaggctac
     1021 tgctttcttc aagttctcca gcatatccaa ttatcattga aagtggagtt cttatatcat
     1081 gagatattcc tgcaatccag ttagacctag cttcctcttt cttcctaagt ttttcatctt
     1141 tcatttgcaa ctctttagat acagtattaa tagattttga aatatcactt agtattcctt
     1201 tttcttgtaa aagtactggt tctccttttg acataagctt tatgccagta ataattggtt
     1261 ttatagattt caccattttt aaacctatga atagagctat taaaaaagta actattgcat
     1321 taaccaaaaa catacctatt attaacatag gaaattgttc tatttcctta accgtataag
     1381 taaggttaaa cttagtgtac ttgtcttttg gataacctgt tactaatatt ccatttggat
     1441 gttcccatac atacacagga tagtccttta aaaagaacct tgaaaacttt gctacatctg
     1501 tcaaagtata atgacttgga atttcttttg gtaaatcaaa tccccatatt ctatctccat
     1561 tgttatcaat taacattgcc caaactttat ttttatgtag tacttcctta ctagaatcat
     1621 ctaaaataaa ctttccattt cttaaaatca aattatcttc aatcataatc aagtttgatt
     1681 ttatttctct gctatcactt gtatctaaat tatcagagta tttagataca aatagcatat
     1741 atgaaaacag aagaaaacct gctatattta atacaataaa aagtaatgaa atcaagatac
     1801 ttgaacctat aaatcgcctc aatatgtact ttacactatc caaaattaaa tcactcctct
     1861 tgatttcttt tattctaata tctaaaatta attaatcatc aacttatatc caagacctct
     1921 tactgtaata agatgtttag gtgtagatgg agttttttct atcttctctc taacccttct
     1981 aacatgaacc ataagggtat tttcatatcc atacatatca tctccccaaa tggcttgaca
     2041 tagtgaatca ttagtaacaa ttctcccttt attttcatac aactttaaaa gcaacgaatg
     2101 ctctttagct gttaaagaaa tctcttgccc atctttctca acacatgcac tatttaaatt
     2161 cacaacaaca gaatctccca gtttaaatac tggcaactcc tcttcttcta taggcacata
     2221 tacacgattt aatataccca caagccttaa agtaagttcc tttggtaaaa atggctttac
     2281 tatataatcg tcagccccta agcccaaacc aatcaatcta tcttcatctt cacctcttgc
     2341 agagagaaaa atcactggta ttttagatgt ctctctaatt tttctcataa tagaaaaacc
     2401 atctccatct ggcaatacaa catctaaaat tgcacaatct ggctttacat ttctaaatac
     2461 cctaacagct tcctcataac ttgatgctaa aaatactcta ctaaacccct cttttctcag
     2521 gatttcatct atcattttta ataattcctt gtggtcatca accacaagta tttttttatc
     2581 ttttattgac tccataatca tcaccctcct ttagtatacc atatacccat tttcacaact
     2641 ttgcctaaat agacttttaa ggttattgta aggtaaattt cagctaattg aaatgtaacc
     2701 aagttattat actgatataa gttaaaagaa tcaataagca cagattaaaa aaggggatga
     2761 aacatatgga aatgataatt agaacacaaa gactttcaaa ggaatacaat aaaatattta
     2821 gagtaaagga tatagaccta agagttccac aaggagctgt atatggtttt ttgggtccta
     2881 atggagctgg aaaatctacc actcttaaaa tgcttcttgg acttgctaaa cccacacaag
     2941 gaaatattaa tattttaggt aaagagttaa atgaaaaaaa tagaatatca attttaaaag
     3001 atataggttc tttgatagaa tctccatcat actatggtca tcttacaggg cttgaaaaca
     3061 tgaccataat gcaaaaactc ttagatttac ctaaaaaaaa tgtaaatgaa gcattaaaaa
     3121 ttgtaaggtt agaaaatcaa aaaaataaaa aagttagtca atattcttta ggtatgaagc
     3181 aacgtcttgg tattgctatg gcaataatga agtttcccaa attattaata cttgatgaac
     3241 ctacaaatgg actcgaccca gcagggattg aggaaattag agaattaata aaatctctac
     3301 cagaaaaata cgggatgact gttctaatct ctagccatct tttaagtgaa attgaccaaa
     3361 ttgccacatc aataggaata ataaatcatg gcgaactggt ttttcaagat agcataaaag
     3421 aacttcataa tagaggtcaa agtcagattg ctatcaaaac taagaatgtt gtatctgcac
     3481 aaaaactatt aaatactaaa ggatatatgt cacttataca agatgggtac ttgactattg
     3541 aaaatttaaa agactcagat gtggctaaaa taaatggtat gcttgtccat tcaaatatag
     3601 atgtatttag aatagaagaa cataaaaaaa gcttggaaga tatattctta gaacttactg
     3661 gtaaggcggt gactttataa tgaaatacct tggtctagaa ttttataaac ttaaaagaaa
     3721 gaagattatt ctgatgacct ttttatttgt agctgttgaa ataatgtggt gtatcataaa
     3781 ttcaaataga gctattacac gtaatccaga tatgttaggt ggatttgaat attcctatat
     3841 gtttatgagt tttgcaagtt taaatggtct attctttcct atcttaatat caataattac
     3901 atcacgtata agcgatatag aacataaagg agatacatgg aagcttttaa aatcttctgt
     3961 gacatcttta aatagtattt atttatctaa atttttatgt tctgctattt tggtatctat
     4021 acctgtattg atgcaagtgt tatctattgt gttgtttgga cattttagag gggttattga
     4081 gtcaatttct gtatctttac ttttaaaata cacattagga acaattcttg taagtattgc
     4141 tatcattgca ctacacctat ggattgcaac tgtaattcca aatcaaatga ttgcaattac
     4201 atttggcatg attggtagtt ttataggttt gacatcaggt ttgttttatc aaggtattcg
     4261 tagattgttt atatggtctt attacctaga gttatcacct ttaagttatg cttatgataa
     4321 tgttttaggt tcatctgtct atagaattga tatgaatttt tctattacga ttttagtttt
     4381 cttaattgga atgctaattt attatattgg aagaaatcat ttattaaaaa aagaagtata
     4441 ggagggattt aaatgttaag gaagtcagtt ttgggagaaa ttctaaagtt aaaaaattct
     4501 tttatatggt atgtattatt agctttgcca cttataagtg tgttgattgg ttctggtaat
     4561 ttttatctaa atcaaggcat actcaaaaaa gaatggtaca gcttatggac tcaagttagt
     4621 cttttttatg gggaattttt ctttcctatc ttaatcgcaa tcttttgtgc atatatttgt
     4681 aggcttgaac atatgaatca taattggaat aatgtaatga cactgccagt taatttaaaa
     4741 aatatctttt tatctaaact tacagtagtt tcaatactta cagctattac acaagttttc
     4801 tttgttatct tttatatagt tgcaggaaat atgtttggtt tttctggtag tgtacctaaa
     4861 gaattgactg gttggattgc taaaggttgg attatttcta tttcaatagc ttctatacaa
     4921 ttatacatgt ctataaagat tagaagtttt gcaactccta ttggtattag tctttgcctt
     4981 tctctatttg gtatgggatt aatagtagca aaacctgctg ttggtatatt tttcccttat
     5041 tctatgttag gtagtgctat gggggttata aagcaatctg ctttaaatgt taatgacact
     5101 atagttacaa tatgtgttgc atttattact actatagtat tttctgaaat tgccaataga
     5161 aattttagaa agaatgatgt tatttcttaa tggtaaatgc tagatatttt ggggtttgaa
     5221 atttatattc tttttagttg aaatctatat ccattttatt taaaaatata tatcttttat
     5281 ttgaatt
//
LOCUS       DACULQ010000213         5257 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.215, whole genome shotgun sequence.
ACCESSION   DACULQ010000213 DACULQ010000000
VERSION     DACULQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.215"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            31..867
                     /locus_tag="JA154_16735"
     CDS             31..867
                     /locus_tag="JA154_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAT7997478.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1220..2797
                     /locus_tag="JA154_16740"
     CDS             1220..2797
                     /locus_tag="JA154_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAT7997479.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGIANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2790..3974
                     /locus_tag="JA154_16745"
     CDS             2790..3974
                     /locus_tag="JA154_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-cystathionase"
                     /protein_id="HAT7997480.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            3992..4591
                     /locus_tag="JA154_16750"
     CDS             3992..4591
                     /locus_tag="JA154_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HAT7997481.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4615..5085
                     /locus_tag="JA154_16755"
     CDS             4615..5085
                     /locus_tag="JA154_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAT7997482.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         1956 a          556 c         1015 g         1730 t
ORIGIN      
        1 atgagagaat ttttttagag gggatttata gtgaatataa aaaagatatt taataacaat
       61 gtagtagtaa gctccctaga agatggcaca gaaataattg tcactggtgc tggagtagga
      121 tttaagaaaa aagtaggcga tttgatagat gaaaatttga tttctaagaa atattttgtt
      181 caagacgatc aaagggacaa atacaatcaa atacttaata agacctctat tgaatacttt
      241 aaaatttcag aagaaattat agaaaaggct aatgaagttt taaatactca agtgaatgat
      301 tcaataatat tagccttaac tagtcacata gaatttgctg tccaaagaga aaaacaaggt
      361 ataaaattac caaacttaat attgaatgag acaaaacaac tttataggga agaatttgaa
      421 tttggattat gggcaattga tgaaattgaa aaaaggatag gaataaaatt gcctgaagat
      481 gaagcaggat atattgctat tcacataatc aatggtttgg aaaattctca aaaagatgaa
      541 ggtattaata tactagagtt ttcaaaacaa gttattcaga taattgaaga agtacatgga
      601 tttaaattgg atgtaaattc attaaattat actagactta taactcactt aaagtttttt
      661 gttcaaagga ttctccgaaa agaaacttac aatgatagtg atattgaaga tatgtataag
      721 ttagtgaata aaaattacaa taaacctaaa gtgtgtactg aagaaattgc agcattggta
      781 ttagacagat ttgaatataa gattagtaaa gaagaagaat tatatttaat gattcacata
      841 aataaaataa caaacagtgg ttgttaggat tgtaactgat aaagcaggca aaacctaagt
      901 cattgagctt aactattaga ttaagttttt tgatttaggt ttttttatta gatacaatca
      961 aaatacagac agtgactttt aggattgtaa ctggttaagc aggcaaaacc taaatcattg
     1021 aatttaacta ttaggttaag ttttttgatt taggtttttt tattagatac aatcaaatac
     1081 agacagtgac ttttaggatt gtaactggtt gagcaggcaa aacctaaatc attaaactta
     1141 attattaaat taagtttttt gatttaggtt tttctataaa tttgattatg tttttagata
     1201 aaaataggag gggaaaatta tgaaaaaaaa taagttaggt ttatggaact tcttccaaat
     1261 gttaggaaaa acgtttatgt tcccaatagc actacttagt gtttctggga tgatgctagg
     1321 acttggggca ggttttacag accctaagat gattgaaatg gttccatttt tagggaatga
     1381 atatgttaac ttagttttaa acttcatgtt aacaatagga ttatttgcat ttaacaactt
     1441 gggagcatta tttgcaatgg caattccact aggattatta aaaaaagaaa aagagtttgg
     1501 tgctttttca ggattagtag gatttatagc aatgcatata ggtacaaatt tttatttaac
     1561 aagagctgat ttgttagtac cagcagaaca gatgtcaact aatggtcaag ctatgattat
     1621 gggtatacaa acatataata caagtgtttt aggtggtatt gtagcaggat taattgttta
     1681 tgcaatatat gataaagtta gtaatttaaa gataccagaa tcattaggat tctacagtgg
     1741 accaagatta gttccaattg ttagtttaat agttatgagt atagttggat tattaatacc
     1801 tattgtttgg ccaccatttt tcaatgcatt ccaaagttta ggaagatgga tttcttcagc
     1861 aggaccatta ggatattttt catatgcagt ggcagaaaga gttacaattc catttggact
     1921 aaatcatctt gtaacatctg tattccgttt tacaccaata ggtggaactg ctgttattga
     1981 tggagaaacg tactttggta cattgaacat gtttatggca tatgtagaaa ataatcagat
     2041 tatacctctt gatttagctg gtaaaatgga acaaggtaaa ttgatgattc aatatggatt
     2101 agctggtgct gcattggcta tctaccgtac agcaaaacca gaaaatagaa aggcacttaa
     2161 aggactttta atatcaggag tgttgacagt tattataggt ggtataagtg aaccaataga
     2221 gtttctattc ttattcatat caccagcatt atttgctttc catacattta tgaatggtat
     2281 agcaaacatg gtacttccat acttaggcgt gttgatggga ttcacaggag atttaattgc
     2341 atttataagt tttggtgtgt taagaggaac agctacagga tggccaatag cagtattagt
     2401 agcagtttta tattttgcaa tatactattt tgtatttaag tgggcaatta ttaagtttaa
     2461 tataaagacc ccaggacgtg aggataaaga aataaaagta tctgaaggaa atggttcaga
     2521 tttcaaaaat ttgtctacat ataaaggaca acaaatgata tctgctttag gtggtcaatc
     2581 taatatatta tcactagata attgtgtaac tagattgcgt ttaaaactac aagatgtatc
     2641 attaataaat gaggatgcaa ttaaagaggc tggtggtata gcagttgtta aattagatga
     2701 gcatacaata caagtaatta taggaactca agtgtattct ttaagaaagc agatggataa
     2761 agctatggaa ggtcatttgg aggcatgtaa tgattaattt tgataaaaaa gtagaccgat
     2821 tgggtacata ttgtacccag tgggactatg tagaagatag atttggtaag aaggatttgc
     2881 ttccttttac aatttcagat atggatttag agtttccatt tgaaataaca gaggtgttgc
     2941 aggaaaggtt aaatcataga gttttgggat acagtagatg gaaacatgat gattttagaa
     3001 attcagttac gaattggtat attaaaagat ttaattgtag cgttgatagt aactggatat
     3061 attatagtcc aagtgtaatg tattcaatta gcaaacttat tgagatgtta agtaatgaag
     3121 gagatggaat ccttataaat actcctgcat acaatgcttt ttatgatgtg ataataaata
     3181 acaatcgtaa tattataaag tcacatcttg taaataggaa tggattctat tcaattgatt
     3241 ttaatgattt tgagaaaaag tgtaaaaaat ctaagatatt tattttatgt aatcctcaca
     3301 atccaactgg tagagtatgg agtgagttag aacttgttaa gatgatagga atttgtaaga
     3361 ataatgatgt caagataata tcagatgata tacacatgga tattgtatat gacaatagaa
     3421 tgacacctat cttaaatgtt gcaaaggact atttagattc tgtattcatt tgtacatcag
     3481 cgtctaaatc tttcaatata ccagcattga ctgggtcata tgtgattata ccaaatgaag
     3541 gtattagaaa taaatttgaa aatatcacta gataccgtga ttttgtaaat tcgccagcaa
     3601 ttctagcaat attagctaca atggtctgtt atgatgactg cgaatattgg ttagatgaac
     3661 tattaaaata ccttaaagag aatcttaaat atacgattga ttatataaaa gataatttag
     3721 aaccattgaa attggtcatg ccagaaggct gttactttgc ttggatagat ttttccgaac
     3781 ttaaaataag tggtgagaaa ttccaaaaat tattgatgga tattggagaa gttgctatta
     3841 tgtcggggtc tgtatatggt gaagaaggtg agttttattt aaggttaaat gtggcatgtt
     3901 caagagaaaa acttgaagat ggtttaaata ggattaaaaa aactatggat tatattgcaa
     3961 aaagaatttt atagtaggaa gggagatagt tatgggatat ttaaaaacaa tggttgatgt
     4021 tatgactact gaaatgaatg ccataagatg tttaatagat gaagcaggaa tagaatatga
     4081 aagtatagtt agtgaaattg caaattgtga aggcaaagta atttttatgg gtgtaggaaa
     4141 atctgcacat ataggtaaga aattggcagc tacatttgct agtactggta cacctagctt
     4201 ttttgtacat gcaacagagg cagtgcatgg agatttagga atgattgaaa gtaaggatat
     4261 tacaatttta atatcaaata gtggaaattc tatggaagtg gtaaactgta taaaatatat
     4321 taaagcaata ggttctaaga caattgcatt tacttcaaac agaaattcag tactggcaaa
     4381 agagtgtgat tatgctttaa tatatcctgc aaaggatgaa gcagaccatt taaatctagc
     4441 accaacaaca tcatctacaa ttactttggt tttaggagat tctattgcat gtgcattatc
     4501 taagagtagt aattttggtt cttcagattt ttataagtac catccaggag gaagtttagg
     4561 tgagaagtta aaaacagcta acaatggata aaagcaaaaa ggagagtagt ttttatgttc
     4621 aaaatgttta aaaaaaaatc aataaaatca cccatatcag gaaaagtaat agagttatct
     4681 aaaatacaag atggtgtatt ttctcaaaaa attatgggag agggtgtagc tatagattct
     4741 acaggagaca ttgtatatgc tccaatcaat ggaaagatag tagtagtagc agagactaaa
     4801 catgcatttg taatagaatt agaaaatggg atggaacttc ttattcatgt tggtttagat
     4861 actgtaaatt taaaaggcga aggttttgag gtattggtga gtatgggaga acaagtgaag
     4921 gaaggaacac caatgttgaa aattaaccgt cctttgattg aatcaaaggg tatatcactt
     4981 attactccag ttacaataac taatcattca gagtacaata tgaatacatg taatgtaggc
     5041 aatagtgttg aaggtgggaa agatactgta attgagttta aataaagaac taaaatacaa
     5101 ggttaatatt tataaatacc tataaactta tgttttataa gattgattgt atagatttgt
     5161 tgtatagatt tgttgtataa aaaagaactc tttaattata aattaaggag ttctttttta
     5221 ttttgtaaat attgtttaga tttatataga aaaacac
//
LOCUS       DACULQ010000214         5215 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.216, whole genome shotgun sequence.
ACCESSION   DACULQ010000214 DACULQ010000000
VERSION     DACULQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.216"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(279..659)
                     /locus_tag="JA154_16760"
     CDS             complement(279..659)
                     /locus_tag="JA154_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT7997483.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(860..2053)
                     /locus_tag="JA154_16765"
     CDS             complement(860..2053)
                     /locus_tag="JA154_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAT7997484.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGIYLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            complement(2116..3336)
                     /locus_tag="JA154_16770"
     CDS             complement(2116..3336)
                     /locus_tag="JA154_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel protein"
                     /protein_id="HAT7997485.1"
                     /translation="MIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLIDTIFGTVLLKVT
                     DIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGEEEIIPLRLVPFI
                     ISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLVTGMAAGFAGLFE
                     TPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFSFALSSKVVFDLS
                     IFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGVCLSVLFLLFYKG
                     RYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTPLFSIGASLGVLL
                     AGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYFFVVCAISYIFNM
                     DKSIYSLQKISTKQ"
     gene            complement(4168..4902)
                     /locus_tag="JA154_16775"
     CDS             complement(4168..4902)
                     /locus_tag="JA154_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT7997486.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
BASE COUNT         1873 a          905 c          543 g         1894 t
ORIGIN      
        1 atttttaatc aaatgaacga atattatata tatcattcat aaaaacttta tataaagaat
       61 agaaacaagt tctagattac tatagagatg acatatcaaa atatttttaa aaagcaattt
      121 aagtattaga agtaattgaa gttagtaaat ataaatgcgt ttacttttaa gtattgctta
      181 agggaataaa aaataaaaat aaactctgca accatctaaa aatagttgca gagcttacaa
      241 aaccaaaatt atctaaattg taataaaaat aataacattt atacataagc ttcttcccaa
      301 tttactaccc agttttctaa cgactttatc ttttcctctg ttagctcctc ttcgtgaaat
      361 gatactaata aattccttat aaaattattc ttcaaaagat tttgcacatt tcttgatata
      421 aactctaaat atgccttttc tttgattgca actgtataat acatacactt gcctgtttct
      481 tgaacatata agaatctttt ctttgatagt tttgataaag tttttaaagc tactttttct
      541 ggtaaattat gttcttcttt catataatca ataatctcat atgaattggt ttttatatct
      601 aaattccaaa taaatttcat aataactaac tctaattttg ataatttttt taaacacata
      661 gttatcactc cgcttcttta tctatataat taaatattac aataataatt tcacaaaatc
      721 aattctaata atcataacat accttaaaat ctgttcttaa cttctacctt cttaatctcc
      781 caaatgattt ttttatattg tattcaatag tataatcctt ttccaaaata taacttacca
      841 gttaaaataa tctttatttt tacttattct cgccttttac agaagttaat aattttttat
      901 aagttacatc aattccaaaa gctccaagag gtgcagttat aagtatagct agaactgcta
      961 acgtcaaaat aagttgtcca gatgccatcc ccattgcaag tggtacacct cctattgccg
     1021 cctgtactgt agcctttgga atatagccaa tcatacagaa caatctttcc tttattgtca
     1081 aatttgtctt aataaggcag aagaatactc ctaccatacg gaataaaaga actccaaata
     1141 ttaaaattac agctccaatt cctgctttaa atgcataact tatatctact gttgccccta
     1201 ccaaaacaaa caagagaatt tcagcaccaa cccatagttt cgaatacttt atagacaatc
     1261 ttcttgcaac tacacgatat tttatctgaa ggaaaatccc aatactcata actgctaata
     1321 atccagaaaa tggcacaata ccctctaaag cagtctctaa acttatcatt aaaaatgaga
     1381 tacttaaaag caacacccct ttagcagaat ctctcatatg aaccttctta aaaaatactg
     1441 ctaaatagat tccaatcact gctccaacta ttacacctaa tataattgat actggaattt
     1501 gtaaaaagct aatagctgat atactatttc cttgtgccaa tcctgtgaat actgtaaaca
     1561 tcacaattac gaaaatatcg tctatagaag ttcctgctaa aagcatctgt ggaatacttt
     1621 tttctgtacc ataaccttct tccattaatt taagcatttt aggtacaata attgcgggag
     1681 aaactgctcc tactactgca cccatgactg ctgcttccaa tatagaaacg cctagtaact
     1741 ttggtgctaa aactatcatt ccgataattt caaaagtagc tggaataaag cacataagta
     1801 cagcgggtct tcccactttc ttcaaatcgt ttatatctaa agataatcca gcccttgtta
     1861 atataataat caatgcaatc tttctaaggt cagaagaaat atctagtatt gaattatcaa
     1921 tcaaatttaa aacatatgga cctaatataa ttcccgtaag taacattccc aataagttag
     1981 gtaattttat cctcttaaaa atacctcccg aggccattcc taataaaaaa atcaaagcta
     2041 aacttgtcaa catattccta ccctatcctt tctctaatat tctttctctt ctaatcatac
     2101 attatgttgt tttatttatt gttttgttga aattttttgt agtgaataaa tagatttatc
     2161 catattaaat atatatgaaa tagcgcatac tacaaagaaa taaggtaaat agctgtaccc
     2221 aaaaacttct gcccctataa atacaggtgc aaaaaaagta tttgtagcac taccaaagac
     2281 actagcatag ccaagcgctg caactaattc aattggcaaa ttaaaaaatc ctgctaacaa
     2341 tacaccaaga ctagctccaa tggaaaacaa aggtgtaacc tctcctcctt gaaaacctgc
     2401 tgacaaagtc agtattgtaa gtataaactt taacagccag tcaaatgaat aaatttctcc
     2461 accatagaaa ctattttgaa ttaaatttgt ccctaaacct gaatatcttc ctttataaaa
     2521 taaaaggaat aatacactaa gacatactcc aataatagca atccttatca taggattttt
     2581 taatctattc cctatttttc tttttgacaa tttcagacac catgcaaatg ctccaccaac
     2641 cattccaaaa ataattccta aaacaataag cttccaaaat attgacaagt caaatacaac
     2701 tttagaagaa agtgcaaaag aaaatttctc taaccctaaa gcttttgata ctgcacttgc
     2761 tgtaaaagaa gcagtaaacg caggaaaaag cgattgatac tccagtgaac cagcaaccaa
     2821 aacctccata gcaaatagta ttgctgctat tggtgtctca aataatcctg caaaaccagc
     2881 tgccatacca gttactagaa aaatactaga agcatttttt ataggcaacc ttttacctac
     2941 ccaatgtgaa aaagttgcac caatctgaac agcaactcct tctcgtcctg cactaccacc
     3001 aaataaatgg gttaaccatg tacctgatat tataaatgga accaatctta aaggaataat
     3061 ttcttcttct ccatgcccta cctcaaatat tagattcatt cctttactac tttttcctcc
     3121 aaatttaaaa tagcaataag caataacaac tcctactact gctaaaaatg gtatcagata
     3181 cattggatat gtttctcgaa tatctgtaac ttttagaaga accgttccaa atatggtatc
     3241 tataagacct ataattgcac caactggtat acctatgagt cctaaaaaaa atagtccacc
     3301 atatgtttta taaacgcttt taattcttat tatcatttta atccatatta aagttttaaa
     3361 tttttctatc attttaattc ctttctattt gcatcataag attttttgaa agttttatta
     3421 catcaactgt agtcaatgta atgactatca taattgtttt ttaaattgat aggatatagg
     3481 tacactctta acccattctt aataaaataa ttaacatata atctactatt ttttctatta
     3541 attaaatatt tcttcaaatt taaaaaataa aaatttagta agtacttcac ctccatttta
     3601 tcgatttaaa caacaaaaaa agctggtggc atctatgata tcatagaagt catcagctca
     3661 ttggcagttt aggatattat catccaaggg agaacctcat tcccataatt attatataat
     3721 tttttatttt tcatatgtct attttatgta aattgtattt ttaatgttat aaataatata
     3781 acctatcaat taaaatatta aagtctctaa tattatgagt gttcaatact tcatattatt
     3841 tttatgatat ataatttaaa tgattttctt tactttgcat tatgatacat gatttaaata
     3901 tttttcttca ctttgtatac taaagtttat ttaaaatatt ttctttccta ttatactatg
     3961 ctgttaaata tttttcaact gaagtattgt agaactttaa aattactata aaaaattata
     4021 ttctaaaata cttgtaatac tacatatcac ccttaacact taagtataat tttaaagcaa
     4081 aattcttatt acttatattt aattttgatt ctacatcttt agatataatt ttaagtaatt
     4141 taagaaatta ttgtaaatca tatagtacta ctcaataatt aggtttttca atgttccaat
     4201 tatgtttctt tgatataatc caccatgact aaaaactatc gtttctatat cataattact
     4261 tatattttta atagcatctt ttaactcttg tttgttgctg tacatcacat caacaggctt
     4321 aagttctcca ttttctacct gtaacaaatc tccagcaata agtactttag actcttttaa
     4381 ataaagacat atgtgacctc tagtgtgacc tcctgtatgt ataacttcta tttcctcacc
     4441 tagattcaat ttttcatggt tatctaatga cacatctact ttagtatatg atgatttaaa
     4501 tccaagtccc ataacattta gcatactaag catcttatca tcaattttat ctaccatttg
     4561 ttccaacata taaagtttaa aaggagtttc ttctcctgtt atatagctga tttcactttt
     4621 gtatgcatat actttgatat tattcttaga tttttctctt agataattta tgtttcctat
     4681 atggtctata tcatgatgag tgattattat attttttaac ttatcaaagc tcgtgttact
     4741 tttattaaga gcatcaatta ttaaatcacc ttgacccgga agacctgtgt caataagtgt
     4801 tgcatcatct tctgtgtaga ttacaggaat atacataact ttatcttcac cgaatacatt
     4861 tgatgaaatt tttaaaacat ctaatccctt tgctaaattc ataatttttc tccttataat
     4921 tacttaatta gaggtcaatt taattaaaga aattgagaaa atgtagattt tcctctagtt
     4981 atattttatt aattaaaaat tattaatacc tagtatatca tttaagttga aggattactc
     5041 gacttgattt ttttgttatt ttatggtata gtaattaata tagaagtaag taattgcaag
     5101 ctttgcaatt acttttattt ttttctaata actattttat ttttgataat tcgtataaaa
     5161 ttaaaagtaa agtaatatag aaaatatgaa tatagaaaaa ctttaataag aaaat
//
LOCUS       DACULQ010000215         5169 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.217, whole genome shotgun sequence.
ACCESSION   DACULQ010000215 DACULQ010000000
VERSION     DACULQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.217"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..803)
                     /locus_tag="JA154_16780"
     CDS             complement(<1..803)
                     /locus_tag="JA154_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997487.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKS"
     gene            complement(926..3469)
                     /locus_tag="JA154_16785"
     CDS             complement(926..3469)
                     /locus_tag="JA154_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HAT7997488.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(3747..4081)
                     /gene="rnpB"
                     /locus_tag="JA154_16790"
     ncRNA           complement(3747..4081)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JA154_16790"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4213..4890
                     /gene="srtB"
                     /locus_tag="JA154_16795"
     CDS             4213..4890
                     /gene="srtB"
                     /locus_tag="JA154_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HAT7997489.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(5050..5124)
                     /locus_tag="JA154_16800"
     tRNA            complement(5050..5124)
                     /locus_tag="JA154_16800"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5090..5092),aa:Thr,seq:tgt)
BASE COUNT         1577 a          875 c          500 g         2217 t
ORIGIN      
        1 gactttacaa tagtcttctt agatttctta tttataaaaa ttaattggtc ttgatatttt
       61 actaaaagca tgtttgtgtt tttataatca aatccatcta tatttcctga tatcttgcag
      121 aatactattt cacaatcttt aggaagattt cctatttttt ggttatcttt aactctctca
      181 tttaaatcag atgggactac actatctata ttgttattaa atatagatac agaccttact
      241 ataatcccct ctctttccat atgaaagtat cccttcaatg acactctttt atcttctaaa
      301 atgtatcttt ctatatcatc aaaatttatt tcaaatctat ttttcccact gataaatata
      361 aacttctcat tatctttatt aattccaccc atataaaact tattgtcatc taaacttaat
      421 atagtcatat tatcctttag atagaatata aattctcttt gacttttgca gattaaatca
      481 tattcatcca aagttatttt catagtcaaa tttgctgtaa catttttact tgaattttca
      541 ctttttatgt aaaaaggcaa gttatttcca tagacaatta tttgagaatc ttctaaaact
      601 gcctctaatt tttctttatt gtctgaaaaa ctatatatat attttacacc tgtatcctca
      661 ttaacttcat ctgatttgct gattattata ttccctagat ttgttatatg aagcttttta
      721 tattcttttg agtatctact tatgccacta agctcaaacc catctgaaag ctcatatatg
      781 attttttctc ctaatttcaa cataccttca ccacctaaac agaattataa gtattttata
      841 aaggtgaata aaatgtctca aaacaaataa tatttttttt aatacttttt attctgagac
      901 atagtttctc cttttttata atgctctaag caccttcaac tatttcttca taaagtttat
      961 ctaaatgttt aacatattca cgatatccag atttgttctt taactcactt cttaaatctt
     1021 catatttttg tttaagttct tcagcactca aagaaggagc tgattctttt aattcttcta
     1081 acttataatc atagtactta tcatacatag ctatatatgt atcaatagac ttcttagcta
     1141 gttcattaca tttttcttgt gcttctccat attgagaata atcacctatt tttattttat
     1201 agtatccatt ttcttgaatt attgttgaag aaataccttt agcttcatat tcttttgctg
     1261 tttcttttgc tgttttttca actttaagtg aacctgcata aacagtgtaa tatacacctt
     1321 taccatcttt atatgcttcc atttcttttg ctttcttttc agcttgtgct ttttcttgag
     1381 cctctttttc agcttttgcc ttttcttctg cttctttttt ctctttttca gcttgtgctt
     1441 ttttctcttc ttcagttaat ttattgttag cttgttgttg agttgatgac tttggaatat
     1501 tctctttatc atttttctta ttgttgccta ctgttataaa atatcctaca ccaacaataa
     1561 ccactataac tactgctata atagtttctt ttaatctact cttcttagaa acaacctctt
     1621 catcatctaa agaatcttct ttatctgata cataattttt catttctttc ttgttttctc
     1681 tatatttgtc cttagaagaa tttttagatt ttttattact attttctttc tttttaatag
     1741 gtattactac tgatttttca ttttctaatt tttcttcttt tatttctttt gcttttttat
     1801 cttctaagtt tttactttct ttttcaagct tcgattcatt atttgaagat actttttcat
     1861 ctaagttatt atttattggt ttttctattt ttacactacg ttcttcttct atatgttgac
     1921 ttggttcttc ttgttttatt tctttcaatt cattttgctc tatttcttct gatttcactt
     1981 gatgattttc aactggttta tctaaaatta tatctggaga aatatcattt aaattctctt
     2041 cttcttgttc agcttcttca ttgtcttttt taataaatcc aaataattta gccttctttt
     2101 tactcttttt attttctggt tcaattatat cttctttatt gctgtcgtac tctttatctg
     2161 tagtatcttt atcttctaca acgtcaccta tatcttgatt attactatct acttcttctt
     2221 tagtcccttc ttcagcatca tcttctattg aaatactact tctaaagcaa atatctttat
     2281 ctgcattttt tatcatgtaa atgtattctt caatatcgat atctttattt cttctagttc
     2341 tttttgatgc aatttcatga ttttctaatt cactatagtt atcttttatt agattctgtg
     2401 ctttattcca tgtatcttca ctaatttctg ttttattttt aattacatag tccacaaatt
     2461 ccttaaaatt ttcaggacca aattctccaa ttatagtcag tctcattgca ccttctataa
     2521 ggtctctatt tcttctcttt aaaaattcta atacaactgt tgcacaagca aaatctttaa
     2581 taatttcttt gccaaaacca tatgcttcaa cagaatgaag ttcttctaat ccacataact
     2641 tcttatattc attgtgtatt gtttgagata tactttcatt atttaagcta tttaacttat
     2701 ctataaactt cgattctata tcatttgatt ttgattcatg ttttactttt gaatttttaa
     2761 tttcatttag tagcatgtca ttttctgaat tatctttacc taattgcaaa tcctcaattt
     2821 cagttgcttt tatttctact ttgttttgtt cgttattctc ttccacatta ttaggtacca
     2881 catctttatt actagctaat attttgttta cacagaatat aatttctctt tttaattctc
     2941 ttttcgcatc tgcttgattt ttaagaaata aatctccaca ttctttcttt tctaaactta
     3001 tttttctaag agatatatta agaaggtctt tatataggtt taaaacctta taatcctttt
     3061 ctaaacgtct tcttctctct gatagttcca ttttatcata agctaaaaaa ctatttaaat
     3121 cattggcttt tagagagtct aatccctctt tcatagcctt atttacatac tttctacatt
     3181 ttgctgtagt taactctaaa tctttttcta attcatctat ttcaggtatg tacttatcta
     3241 ctatccattt gccaataagt ggttctgtat ttcttgctaa tgtcttataa aacaatatat
     3301 taaattttaa aagctctgca tattttgctt cattaaattc tactttttca aatttctttt
     3361 ccaaaacacc atctagcttt tcatttattt tagactcttg ttttaaaaaa cttgctgtga
     3421 catttaaaac agtgtttctt attatattat tttcactcaa aaccttcact ccttaaatca
     3481 attattttat tgtaatattt acattgtgca aagttgctaa tttgattatg atatctaaat
     3541 aattatttac taaatttgct acatcttata tcatatcata ttatttacat cattttactt
     3601 tttcttttta tatctatctt atattatacc atttttaata tctgtactaa cttatattac
     3661 aatattcctc aaaattttaa aagtagatta gacaaattta caaataaaaa aacatcagtt
     3721 taaactgatg tttttgacta tcgaaataat taagcaagtc tataagcgga gttctgtatt
     3781 agataatcat ctttctaggc ctattgtcac caataggctc aagcgaccta cctaccggag
     3841 agtacgagca gcactcttta ttcctatctt ggtcttgctc caggtggggt ttacatagcc
     3901 aactagtcgc ctagctgctg gtgagctctt acctcgcctt tccatcctta ccacaatagt
     3961 ggcggtttat ttctgttgca ctatccttag gatcgctccc actaggcgtt acctagcacc
     4021 ctgctctatg gagctccgac tttcctcatg taagattaca tgcgattatc tggcttactt
     4081 aatatttatt ttacttagtt atgttaacac aaattctttt acatgtcaaa gtaatattat
     4141 actaaaaatt taaatttatt caaaaatagt gtaatataga tatataaact acatttaact
     4201 cgaggtgatc tattgaaaaa attatataga atagtaatta acattatact agtattagta
     4261 atactttaca gtggatttaa tatatactca aaacttacca aatacaatca tgatactaaa
     4321 atatcctcag aacttcaaaa aaaagaatat aaaaaagagg atttatcaaa gattaacagt
     4381 gattttaaat tttggctttc tgttgagaat acaaatataa attatccagt tgtacaatct
     4441 aaagataatt cttactatct tgacaaagat ttttataaaa aagattctat ttctggtaca
     4501 ctgtttatgg attatagaaa taaatctatt gatgacaaaa atatcataat atatggacat
     4561 aatatgaaaa ataaaactat gttcaacaac ctaaataagt ttaaagatgc tgacttcttt
     4621 aaaaaaaata ataaaataaa aataaccttg aatggaaaag aatttttata tgatgttttt
     4681 tctgcttata ttgtagaatc tgattatgat tatctaaaaa caaattttaa caatgaatct
     4741 gattatcaaa attacataaa tgatattact tctaaatcat tgtataaatc tcctattaag
     4801 gtaaatagca atgataaaat agttacactt tctacatgta cctatgaatt tgatgatgcc
     4861 agaatggtga ttcatggtag attgatttaa aactcacaga cacagtttaa aacaataatt
     4921 ttaaactgtg tcatctttta tacctataat ttgtaaatta gaccactaaa agtatttaat
     4981 acaaaataac aaatacatca aacaataaaa aacaataaaa aaagactatg ctaaaaacat
     5041 agtctttact ggagctggtg ataggaatcg aacctacaac ctgctgatta caagtcagct
     5101 gctctaccgt tgagccacac cagcacacct attttattgt aaattagcaa aactaatttt
     5161 taaaagtta
//
LOCUS       DACULQ010000216         5159 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.218, whole genome shotgun sequence.
ACCESSION   DACULQ010000216 DACULQ010000000
VERSION     DACULQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.218"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            105..2939
                     /locus_tag="JA154_16805"
     CDS             105..2939
                     /locus_tag="JA154_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HAT7997490.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            2997..4226
                     /locus_tag="JA154_16810"
     CDS             2997..4226
                     /locus_tag="JA154_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAT7997491.1"
                     /translation="MKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALLIDQET
                     KRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGGSTCKL
                     KDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMIDTYYFN
                     PNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDTKKDFT
                     HYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLGDGNKK
                     NRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGVVSDNE
                     NINPKVVFKNMNYPIHKGDIVGVVKYYNDSGKFVGSVDVKSESNIGCIPLKDKIKIKV
                     AKINKKLEIKNSVCFKA"
BASE COUNT         1941 a          690 c          764 g         1764 t
ORIGIN      
        1 aatacaaaat atctactata aaccttattt taatactgaa tatatattat aatatattta
       61 atgtagctta aatatagttt aaacataaag aaaggaaacg gtatatgcta agaaaagata
      121 aagttctatt atctctacat aaattatgtg aaaatatgac tttaaaagac cttcaatcag
      181 ataaaacagg attcactaca atagaaattg ctaaccattc atctcttgat agaagtaata
      241 ccagtaaaga attaaataac ctatacaatg aaaaaaaagt tctgaaaata tctggtaagc
      301 caattttatt tctacatgct agtattatag aaaatttaat tggccgtact ttgaacagtg
      361 atgaattttc tataaataat tgtaatgagc tacttaaatc aagtaaaaat gactcagaag
      421 atattttttc aacattactt ggacatgatt ctagtcttaa actacctata gaaaaagcta
      481 aatctgctat actctatcct ccaaaaggtc ttaatacatt actaatagga cctactggtg
      541 ttggaaaaag tacatttgct gaaacaatgt acaaatatgc tgtagaaagt aaaatctttt
      601 cttctgaaag caaattcgta gtatttaact gtgctgaata tgcagaaaat gctaacttat
      661 tattaagtaa tttatttggt tatgtcaaag gttcttttac tggtgctaat aaggataaat
      721 tcggcatagt tgcacaggct gatggaggca tattattctt agatgaaata cacagattac
      781 caccagaagg tcaagagatg ctttttcttt tgatggacaa aggcatatac agacgattag
      841 gagaatctga cattgtgcat aaagctaatg tctttattgt gtgtgccaca acagaagata
      901 tacattcttc tctgcttgga acttttttaa gaagaatccc tgttacaatc aacctacctg
      961 ctttacagga taggtcttta aaagaaagat tatctcttat taaatacttc tttattatag
     1021 aatcaaaata caccaatgca actataaaag ttcacaaaga tgtaattaaa tcattactcc
     1081 tttatgattg ttttggtaat ataggtcagc ttaaatctga catacaactt atgagtgcaa
     1141 gagccttctt aaattacaaa actggactta aggataatat agaaatagat ttatcccttg
     1201 ttcctgatta catctatatt ggtcttttaa aattgaatga aaatcgtagt aatctaaata
     1261 aattatcaga acttgattat atgctttttt atgaatttaa tggtaataac ttagaagttg
     1321 aatatacaaa agaactatct aaatacaata tagatacatc aaacaatgga tttaaatact
     1381 ctacagagat tccttatctg gaaaatgata taaggagtat gtttataaat tacatggaaa
     1441 agtactctac aaaattactt tcgatatctt cagtaactca ttctgattat acaagtaatg
     1501 agatttttaa agtaatcaat ccaaagattt actatgctgt tgaacattct ttaaaagagg
     1561 ctgaaaaaat atcgagaaac aaatacacaa aacaaactta tattgctctt tcaatgcata
     1621 taagtgcact tatagaaaat ataaatcaag acacatataa tcctaagtac aatttatata
     1681 atgataaaat tttcttagat aaagatttaa atatagctaa aatagtttta agtataattg
     1741 aaaatgactt acaaacatct ttacctaaag atgaagtaaa atttatatcc atgtttctaa
     1801 atagcatgta tcttgaacca aacaatcaat ctgaaagtat aggagtcata atacttgctc
     1861 atggaaattc aacagcaagc agtatgtgtg atgtagcaaa ttcattgctt ggtacaaacc
     1921 attgtaatgc aattgacatg cctcttgatg taaaagtaag taccatactt gataaaacta
     1981 tagagttggt aaaagaatgt gacgaaggta agggtgtact tcttttaaca gatatgggct
     2041 ctcttatagc ttttggagaa cttatttcag aaaaaacaaa aatcaaaact cgctcaattg
     2101 aaatggtatc tactccaata gttttagaag cagtaagaaa aagtacacta ccagaaatga
     2161 ctttggatga attagctaat tccttaagct ctttaaatcc atatattgga cgagcagcaa
     2221 tcgattcaac agaaataaac tatcctgaaa agtcaagaga gtatgtaata ataactactt
     2281 gtataacagg atatggttca gctatgaaag tagcagactt tataaattct tcacttaaaa
     2341 atattagtaa atacaatatt aatttagttc ctcataatat agaaagtttt aaaatgtata
     2401 agaataaatt tagtaatact aaaattctgg cagttgttgg aagtgtagat tttaatgtag
     2461 ctaacacccc ttttatacac atagaggaac tactaaatgg atatggccta gatatgctaa
     2521 acaaaataat agaaggtaca ttcaatgcca atagtattac aaatataaaa gaaacaaatc
     2581 tatcatacaa cagtttaata ataaacttta tgacccaaaa cttagagctt ttggactctg
     2641 ttaaattatt caactatgtc aattcttcct tatttacaat aatagaatta atagatgaaa
     2701 acttaaagag caaatttaag ctaggatata tactacattg ctcttttatg atagaaagat
     2761 gttttaaaaa tacaactttt ccatataata acattactga attaattagt aaaaatcatg
     2821 aactatatag tttaatacgt gaagccttta agccaacaga agcttatttt aatattttga
     2881 taccagacga agaaattgct tatataatag acatgattaa tgagtatcta aataaataaa
     2941 atttatagca cttattttgt gcatattaat taatatatga aatggaggac gagattttga
     3001 aaggaaaaat aaaacagtta acaatacttg cattaatttt tatatttata actcctgtat
     3061 ttgcatttgc agatactcca ccagtaccaa attcttcaag agcagcacta cttatagacc
     3121 aggaaactaa aagaatatta tttgaaaaaa acattgatga aaaaatgcca ttagcgagtt
     3181 taagtaaaat gatgaccttt ttattggcaa tagaagcagt tgataaaaat caagttaaag
     3241 agactgatat ggtgaaaata gacaagtcta cagcttcagt aggaggttct acttgtaaac
     3301 ttaaagatgg tgatgaaata tctctaggtg agcttatgca gggtctaatg ctagtatctg
     3361 gaaatgatgc tgctatagcc atagctaaac atattggtaa aactgaaaag aattttgtaa
     3421 atatgatgaa caagaaagca gaagaaatag gaatgattga tacgtattat tttaatccaa
     3481 atgggcttcc tatatatact gacccagaac ataaagaacc tcctattgaa aatatgtcaa
     3541 ctgctcacga tatagttaca cttggaaagt atatgtacga ccattatgaa aatcaagtta
     3601 caagaataac cactatgcaa gtatataatg atactaaaaa ggattttaca cattataaca
     3661 caaatcctct gcttgtttca gtacctggtg ttgatggaat aaagacagga tatacagata
     3721 atgctggata ttgccttgca ttttctatga tggtacctaa agatgctaaa aacgaaagaa
     3781 atcatagatt gataggtgtt gtacttggtg atggtaacaa gaaaaataga atttcttctt
     3841 ccgctacttt attgaaatat ggtaaagata atttccattc taaaaagata gcacataagg
     3901 gtgatattat agagactcct tgtgttgatg gtattgatga ttttaagata acagttaagg
     3961 ttgataaaga tttgtatggt gttgtttcag ataacgaaaa tatcaatcct aaggtagtgt
     4021 ttaaaaatat gaattatcct atccataagg gagatattgt tggtgttgta aagtattata
     4081 atgactctgg taaatttgtt ggaagtgttg atgtaaaaag tgaaagtaat ataggatgta
     4141 ttcctttgaa ggataagatt aagattaagg ttgctaagat aaataaaaaa cttgagatta
     4201 aaaattctgt ttgttttaaa gcatagtaaa gcatattatt aaaagggtat gttaaattta
     4261 taaagactcc actttgtgga gtctttttgt tttttacaaa aaattaaaat agtggcaaca
     4321 attgaggatg tgagggagag ttgccactat tttgtatagg gtattatatt atgtttttaa
     4381 gctagtttta ctatcttcta tgtttatata ataactcttg tgtgtgaatt taattgataa
     4441 ttaatatgaa aattatgtga agttttaagt tagttgactt agactattgt aaattttttc
     4501 agaaaaattc tgttcataag tctattagac caccgaatta tttacctata atattccgat
     4561 caaaaaatct attctttttt aaaagtaagg tcatgattat tgtacatata taaaaattcc
     4621 gaaaattaat ctacacacta gtaacattaa gtataataaa tttattttag taaaactatt
     4681 aaaataacct cataagaaac tatagattaa atcaatcaat ctaatctaca gtttcttgat
     4741 tattccttaa tatttataat ttacattatt tgtttatttt aaaactatct gttacaaatc
     4801 tagtcaatgt tgaaaataaa ttcgctctga atatagtaga tgatggttct aatgcagtta
     4861 tctcaccttg tacttttgct atatcttcat atcctgcaaa actttgaact tctgattctg
     4921 tccaccctgc tgtctttgct tcttctattg catcagttgc tgcttttaat gttacttttg
     4981 atgcttctaa attatctgct gttacttttc ctactagttt tgttacttct gctatttttt
     5041 cttctgcttt tgtctttgct tcttctttag cttgtgatga tgattctaat gcagttatct
     5101 caccttgtac ttttgctata tcttcatatc ctgcaaaact ttgaacttct gattctgtc
//
LOCUS       DACULQ010000217         5156 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.219, whole genome shotgun sequence.
ACCESSION   DACULQ010000217 DACULQ010000000
VERSION     DACULQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.219"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            13..2244
                     /locus_tag="JA154_16815"
     CDS             13..2244
                     /locus_tag="JA154_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate acetyltransferase"
                     /protein_id="HAT7997492.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            2404..3138
                     /gene="pflA"
                     /locus_tag="JA154_16820"
     CDS             2404..3138
                     /gene="pflA"
                     /locus_tag="JA154_16820"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087235.1"
                     /note="activates pyruvate formate-lyase 1 under anaerobic
                     conditions; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAT7997493.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3260..4930)
                     /locus_tag="JA154_16825"
     CDS             complement(3260..4930)
                     /locus_tag="JA154_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT7997494.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHKMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESSKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
BASE COUNT         1843 a          756 c          776 g         1781 t
ORIGIN      
        1 aggaaatact taatgaatgc atggcaagga tttaaaacag gaagatggac taaagaaata
       61 aatgttagag agtttataca attaaactat tctccatatg agggaaatga ctctttctta
      121 gctggtgcta ctgaaaatac taaaaaatta tgggacgaag caatggtcct atttaaaaaa
      181 gaaagagaaa atggtggtac tttagatgtt gatacaaaaa ctgtttctgg aatagctgca
      241 tatgctccag gatatttaga taaagaatta gaaacaatag ttgggttaca aacggatgcc
      301 cctttaaaaa gagctgtcat gccatatggt ggtataaaga tggttgaaaa ttcatgcgaa
      361 gcttttggat atgaattaga cccagaaata aaagatatat ttactaaata cagaaaaaca
      421 cacaaccaag gagtttttga tgtttatact ccagaaatga gagctgctag aaaatctggt
      481 ataataacag gtcttccaga tgcttatggt agaggtagaa taattggtga ctacagaaga
      541 gttgctctat atggtgttga tgctttaata gaagataaaa atgagcaaaa gaaatcatta
      601 gaagtttctt gcatggatga ggaagttatc agattaagag aagaaataac tgagcaaata
      661 tcagctttaa atgaacttaa aaaaatggct gaatcttatg gatttgatat atcaaaacca
      721 gcaactaact caaaagaagc tgtacaatgg ttatactttg gttatttagg tgctgttaaa
      781 gaccaaaatg gtgctgcaat gtctttaggt agaacatcta ctttcttaga tatatacttt
      841 gaaagagatt taaaagctgg aatcgtaaca gaagaagaat tacaagagta tatggaccat
      901 tttgttatga aattaagaat ggttaagttc ctaagaactc ctgattacaa taacttattc
      961 tctggagacc caacttgggt aactgagtgt ataggaggta tgggaataga tggtagaaca
     1021 ttagttacta aaaactcatt tagaatgtta aatactctat atacattagg gccttctcca
     1081 gaaccaaact taacagttct atggtcaact aaattacctc aaggattcaa agatttctgt
     1141 tctaaagtat ctatagatac aagttcagta cagtatgaaa atgatgattt aatgagagca
     1201 tactggggag atgactatgg tatagcttgt tgtgtatctg caatgagaat tggtaaacaa
     1261 atgcagttct ttggagctag agttaactta gctaaaactt tattatacgc tataaatggt
     1321 ggtgtagatg agaaatctgg tgttcaagtt ggaccaagat ttgaacctat aacttctgag
     1381 tacttagatt atgatgaagt tatgagtaaa tttgaaccat tcactgattg gttagcaaca
     1441 ttatatgtaa atactttaaa tgtaatacat tatatgcatg ataaatattc ttatgaagca
     1501 ttagaaatgg cattacatga tagagatata tttagaacta tggcttgtgg tatggctgga
     1561 ttatcagttt gtgcagactc attatctgct ataaaacatg ctaaggttaa aactattaga
     1621 aatgaacaag gaatagctgt tgattttgaa atagaaggag attatccaaa atacggaaat
     1681 aatgatgata gagttgatag tatagctgta gagttagttg aaagctttat gaataaaata
     1741 agaaagaata aaacttatag aaattcttat cctactcaat ctatacttac aataacttca
     1801 aatgttgttt atggtaagaa aactggtaat acaccagatg gtagaagagc tggtgctcca
     1861 tttgctcctg gtgctaaccc aatgcatggt agagatacta atggtgcttt agcttcatta
     1921 tcttcagttg ctaaattacc atatgaacat gctcaagatg gtatatctaa tactttctct
     1981 atagtacctg ctgctttagg aaaagatatg actgaaagaa taaacaatct ttctgcaatg
     2041 atggatggat acttcgctca aaatgctcat cacttaaatg taaatgtatt tgatagagct
     2101 actttagaag atgctatgga acatccagaa gaatatccac aattaactat aagagtatct
     2161 ggatatgcag ttaacttcat aaaattaact aaagagcaac aattagatgt tataaacaga
     2221 acattccacg gtaaaatggc ttagtcttta aatttttata ctttaactaa gattactact
     2281 gtgtaaaaaa taaaaataaa aggctatctt atatttgaat tatttctggt ataagatggc
     2341 cttttttaga gaaatattat aactattgat aaggttatta aaatatattg tgaggtgaac
     2401 tctatgatta agggaaagat acattctata gaaacgtttg gtactgtaga tggaccagga
     2461 ataagatata tattattttt tcaggggtgt cctcttagat gcaaatactg tcataacagg
     2521 gatacatggg atattaaatc aggaaaagaa tacacagttg atgaaataat aacggatgct
     2581 cttaagtata cttcttttat gaagttttct ggtggaggta taactgcctc tggtggtgaa
     2641 tccactcttc aaccagaatt tttaagtgaa ttatttaaaa aagcaaaaga aaatgatata
     2701 cacacttgct tggatacatc tggatttgta gatatagaaa ctatagaccc agttttagat
     2761 aatactgatt tggttttact tgatttaaaa catatggttg aggaaaaatc tattgatttg
     2821 actggtgttg gtatggataa ggcattaaaa cttgctagac acttagaatc aagaaatatt
     2881 cctgtatgga taagacatgt tttagttcct ggcattactg atgatataga taatttagaa
     2941 aaactaggtc aatttgtagc tacattgaaa aatgtagaaa gatttgaatt gctaccatac
     3001 cattctatgg gaatacacaa atgggaaagt ttaggtatcg attatgaact aaaagatgtt
     3061 cctgatgcca ctaaggaaga tgtccaaaaa gctagtgaaa taatctctaa atttggagtg
     3121 aaagtatata atagttagtg tagtatatta atcctttata gaagacataa caaaataggg
     3181 taaagtgtaa atttttaata aacttcaaaa cttccacttt gccctatttt tatatactat
     3241 atatctcatt ttatatacat cactttgata gctgaaattt ataaaattca gaggcactta
     3301 caggtcttcc aaataaaaaa ccctgaataa tatctacccc taagcttttt actatatcat
     3361 actcttcata agactctatt ccctcaacac aaacttttaa atttataata tgagctaatt
     3421 taataatata ttcaagaaaa gtatattcat aactattata tgtaatattc ttaacaaaac
     3481 ttctttcaat ttttattata ttaacagaca gttctttaag gtaatttaaa gatgaatatc
     3541 ctgtaccaaa gtcatctatt gcaatatata ctcctatacc ctttaaacta ataaatttat
     3601 catttagtaa atttatatct ggtatccaac aattctctgt taattccaaa attaaaaatt
     3661 caggtcttaa ttgatactct actaaacatt ctactataaa atctctaaaa aaatcttcct
     3721 ttaactgaat atacgataca tttacagata ttttaaagtc tggatttatt ttatgccatt
     3781 ctttacactg ttttacagct tcttttataa tccacttccc aactggtata attaaattac
     3841 tctgttctag tattggtata aattcaacag gagatacttc tccataagta ctcgaatgcc
     3901 atcttaaaag tacctctgcc cctattacct cttttgtaac agcatttacc tggggttgaa
     3961 aaaacagctc aaagtcatta aaattatttt caacgcactc tcttagtttt tgttgcattg
     4021 aaattacctt tagtttattt tcatagagct cttgtgaaaa aaacttaatt ctatttttac
     4081 cactaatttt tgctatatcc aatgcaatat cagcatgttt aaataaatct aaataattat
     4141 ctccatcttc tggatacata gcaacgcctg cagtcactgt gcaatagtac ttacttgatt
     4201 caatttcatg ctgagtattt gtatacatct gtatcttttc atatattttc tctatagttt
     4261 ctttagtaca catagggtaa aaaaatgcaa attcatctcc atctaaccta tacaatctta
     4321 catcttttgg aagatattcc agtacttcag tagcaattgc acgcaacact ttgtcaccat
     4381 aagaatgtcc atatttttca tttatatttt taaaattatc aatatccata ataaacatag
     4441 caccattttt ataaattttc tcttttataa gataattcat tttatgttca aattgttctc
     4501 tattccacgt tcctgtgata gaatcaaatt tatttttttc accaatattc tttattctcc
     4561 cgactagaaa tatagttttt gggtcatccg acacataaac tttacctcta caagaaatcc
     4621 atactatttc tttatattta ttaatcaacc tatattctaa attgtgctca cctttttttc
     4681 catgtagtaa ttcttgtata tcatctttcc atatttgaac atcatctgga taaacaattt
     4741 tactccaaca attgaccaaa tcagattcta tctctttact taagttaaat tcatcaaaaa
     4801 ttgctgaaga aattttaaat ttattcctat ttatatccca aaaaaatata taatcttctg
     4861 tactttgaga taaaagttca aagaaaattt ctatatggtc taaacttaaa gagttcgatt
     4921 cttctctcat cttttccccc tacaatatta ctattagtgt agtttaatca acaaaatatc
     4981 cttatttaga ttatactatt ttactaattt tttgtctcat caaacacatt tccccaactt
     5041 tagttatctt atcaaatctc ttactcttaa ctaagttttt tgagattcat aaagtaattt
     5101 ttctataatc attactggtg atttattttt acctgctaaa acaccatgtt aaatca
//
LOCUS       DACULQ010000218         5083 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.220, whole genome shotgun sequence.
ACCESSION   DACULQ010000218 DACULQ010000000
VERSION     DACULQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.220"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(236..1828)
                     /locus_tag="JA154_16830"
     CDS             complement(236..1828)
                     /locus_tag="JA154_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAT7997495.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDICGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(1888..2613)
                     /locus_tag="JA154_16835"
     CDS             complement(1888..2613)
                     /locus_tag="JA154_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HAT7997496.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSMSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            2799..3482
                     /locus_tag="JA154_16840"
     CDS             2799..3482
                     /locus_tag="JA154_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAT7997497.1"
                     /translation="MKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVDKTYTKDS
                     IIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTRLHKSNVT
                     VTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIELLSRKTT
                     REKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEGLLSFNKN
                     KFEIFYFKK"
     gene            complement(3646..3798)
                     /locus_tag="JA154_16845"
     CDS             complement(3646..3798)
                     /locus_tag="JA154_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid:proton symporter"
                     /protein_id="HAT7997498.1"
                     /translation="MLTVITTITDIPNTILNTTGNTVSSMLVSRLVEGKDWLIDKTAI
                     TTKKIS"
     gene            complement(3856..4917)
                     /locus_tag="JA154_16850"
     CDS             complement(3856..4917)
                     /locus_tag="JA154_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAT7997499.1"
                     /translation="MRDFLKNYKSSIILLACTIVGAIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKGTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKNLMVTQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     IGTAIAMMGDRGNTIRKLLSEGTEAILKVVSIFMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
BASE COUNT         1674 a          894 c          618 g         1897 t
ORIGIN      
        1 tacttaaaat aaaatatata agatattata tatctaagta ttataaattt aatacaaaga
       61 ttcttaaaaa gaatatactg aaagcaggtt tatttttcaa ttattacaaa aaataaataa
      121 agggtcatct caaaattgat tttttaatca aattttaagt ttaacccctt atcttaattg
      181 tggcatactt aaaaatattt taactataaa tatctttaag tatagatatg tttatttatc
      241 ctaaaatctt cttcatatct tcttcaggag tagatatagg tgtcaatgca aatttatcaa
      301 ccaatatatc taatacattc ttagataaga atgctggcaa agaaggccct atatagatat
      361 ttttgatacc taaatgaagt agtgtaagaa gtatacaaac agctttctgc tcataccatg
      421 acagtaccat gctaagaggc aaatcattta ctccacattc aaaagcattt gccaatgaaa
      481 ctgccacttt aattgcacta taagcatcat tacattgtcc catatccatt aaacgaggta
      541 agccacatat atccccaagg tctaaatcat taaatctata ttttccacat gcaagtgtca
      601 atataacagt gtctttagga gcttgtttta caaattcagt gtaataattt cttccaggtt
      661 ttgcaccatc acaacctcca accaataaaa agtgtcttat agctccactc tttacagcat
      721 ctactacttt atcttgtaca gatgaaactg tacctcttgc aaaaccagta gttaatgtat
      781 ctcctccatt aataccagtc atatgaacat cttctctata tcctccaagt tcaagagctt
      841 tttctattac actagaaaaa tctttatctt catcaatatg aaccatacct ggataagcaa
      901 ctacttctgt agtaaatact ctatctctat aactatcttt aacaggcatt aaacagtttg
      961 ttgtaaataa tattggagca ggaatattat caaattcttt ttgctggttt tgccatgctg
     1021 taccaaaatt accttttaga tgtttatatt cttttagttt tggatatgca tgtgctggta
     1081 ataactcacc atgagtatat atatttatac ccttatcttt cgtttgttct aataatttgt
     1141 gtaaatcata aaggtcatgt cctgtaacta caataaatgg ccctttctcg attttcatag
     1201 atacctgagt cggttctggt gttccataag tttcagtatt tgctctatca agcaattcca
     1261 tacattttaa gtttatttct ccagttttca taactagacc taaaagttcg tcaatagaca
     1321 agtctcctcc aactgctatt aatccttcat agaaaaattc atttacgtct ttgtctgtat
     1381 aaccaagtac cattgcatga tatgcatagg cagccatacc tcttaatcca aataagatta
     1441 atgattttaa tgaacgtatg tcctcattat cattccataa atccttcata tcatagttat
     1501 catttctatt acaagttgtt gaacaatctg aacatctagg tacaagctta cttttttcta
     1561 cctcagtttt ttcaatttgt ctttttatag cttcatcatc aaaacttaca ttcgttatag
     1621 tagtgaatag cccctctatc ataattttat ctgtttcact tgttggtcta ttcttatttc
     1681 caatagttgc tcttgaaaga ccaatcaaca ctcctgtaag ttcgtcttgt aagttagctg
     1741 tacccccctt tttaccacag acaccattta tagtacaacc tttacctcct gctgtttgtt
     1801 cacattgaaa acaaaacatt ttttccattt tttattcctc cctttttatt tattaaaatt
     1861 tattatattt ttctataatt cacttaatta aatttctcca tctacaattt ttccatctac
     1921 agatatagta actatttgcc atggtatcat ttttccacta ttttgtagag ctgtcttaac
     1981 agcatttact ataccaccac agcaaggaac ttccatacgt gttactacta tatttttaat
     2041 atcattttct tttaaaatct ctgtaagttt ttcagcatag tcaacttcat caagtttagg
     2101 gcatccgatt aatgtaacct tatttttcat aaatttatta tgaaaatttc catatgcata
     2161 cgctgtacag tctgctgcta ttaaaagact tgcattcttt aaatatgatg cattagttgg
     2221 tactaatttt atttgtactg gccattgatt aagttgagaa ccttttatct ctactatatc
     2281 attgcttata gacatactat ttgaaacctc tctatttatt gacttagact gagaacctgg
     2341 acatccacat gctaaagttt tcttttgtgc tttctttgct tccatatttg cttttactgc
     2401 agcttcatca tattctgctg cttctctata ctcaaatgaa atcgcatttg ttggacaagt
     2461 tggcagacaa tctcccagcc catcacaata gtcatctctt aaaagttttg cttttccatt
     2521 taccattcct atagcttctt catgacaagc ttcaacacat agtccacaac cattacactt
     2581 ttcctcatca attttaatta tttttctcat catttatatc acctcataaa atttttttaa
     2641 tttaaagtat tgatttctta tatatatatt tttaatacct atatttcttt atttcaaatt
     2701 tcttgtagta ggatttttat atccccttgc ttttataaac ttagtatagt acaatgaaaa
     2761 caaattatcg gttgcatatg caacaaggaa gtgattattt gaaaaaattt ttagaaaagc
     2821 atatagacct acttaaaaaa attcctctat ttgaaggaat taaaagtgat gaacttgaag
     2881 aaatgttgga atgtttaggt gttgtagata agacttacac aaaagatagt ataatattct
     2941 ctgttggaag tgaagttact tctattggaa ttatgcttaa aggaagtgct catataataa
     3001 aggaagatat cgaagggaat aggaatattg tagctgaact cttgcctgga gatttatttg
     3061 gagaggtatt cgcatgtact agactacata aaagtaatgt tacagtaacc acaacatctt
     3121 cttgcgaagt tttattcata aagtttaaaa gtgtaactgg tatttgttca tctgcatgtg
     3181 tatttcataa taggcttgtt gaaaatatgc tacaattaat agctgaaaaa aacattttac
     3241 ttcacaataa aatagaatta ctttctcgta aaacaactcg cgagaaattg cttatgtatt
     3301 tttcaaaaca gatagaacaa acaggtagcc accagtttac aattcccttt tcaagaaatg
     3361 aaatggctga ttttttatgt gttgacagaa gttctatgtc aagagagctt ggcaagatgc
     3421 gagatgaagg cttgttatct tttaacaaaa ataaatttga aatcttttat tttaaaaaat
     3481 aaaatagata tttaaacata tttatttcaa tatatactat attagttgat tcaaattata
     3541 aatatcatat ttgaatctta aatttatatt tttacttaaa aagttaatag gtatctaatt
     3601 tattaaaatt aagataccta ttaactttat taattaagtg tacaattagc ttattttctt
     3661 tgtagtaatt gctgttttat ctataagcca atcttttcct tctactaatc ttgaaactaa
     3721 cattgatgat actgtatttc cagttgtatt taatatagta ttaggaatat cagttatagt
     3781 agttataact gttaacagtg gaattgcctc cactggaaaa ccgaagatgc taaccacgag
     3841 catctcacca ataagtcagc cacctgctac aggagccata aatactccaa caacaaatac
     3901 agttataaat aattgaccta tcgttgctgg atttgccatt ccactaccta aaatacctga
     3961 taaaaatgcc acttttatta ttactgctaa aactgaccca tctttatgta tatttattcc
     4021 aagaggtata actgtttctg atatatcatc aggaactcct atatttttac tagcttctaa
     4081 atttgatggt atacatgctg aacttgaaga agtagctagt gccgttgcta ttggttttat
     4141 agcatttctc cagaattgtc taaacccttg ttttcctgca gataaaaaag catacagact
     4201 gtaaaataat aagaaataca tggctgctgt tagtgaataa attacaaaca cccttacata
     4261 tccttgaatt attttacttc caagttcccc aattattgta gctgtataac agcctagccc
     4321 tataggagca taaagcatga atatacttac tacttttaat attgcttccg ttccttctga
     4381 taaaagcttt cttattgtat ttcccctatc tcccatcata gctattgctg ttccaattaa
     4441 aactgcaaat ataactaatt gtagtaaatt actactggaa aataaatctc caaaattttt
     4501 aactgtaata cttgatacta cttggtctaa taacgttcct tttgattcta aagtttctgc
     4561 atcttgagtc accattaaat tttttacact tgatacatct aatcctttag acggattaaa
     4621 taataatgca gatgtaatac ctaaaaatgc aattacaagt gttgttccca taaatactat
     4681 agctgtaccc ttcattattt ttccaaatcg ttccatattt tttatactag caacacctga
     4741 agaaacacta agaaaaacta atggtactac tatcattaat agagcattta tgaataaatc
     4801 tccaaatggt tttagtattg aagcttttgg accaagtact aatcctacta tcgcccctac
     4861 tatagtacat gctaaaagta taattgatga cttataattt tttaaaaagt ccctcattta
     4921 tattttcctt tctaatgctt tatattataa attaccccaa atgctaaaaa aattaaagta
     4981 aattatctaa tgcgcattaa taatttttgt aattttcccc aacagctact ttagcaaata
     5041 ctggaatata tgtcaatttc tatagttttt ttgtattttt aac
//
LOCUS       DACULQ010000219         5081 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.221, whole genome shotgun sequence.
ACCESSION   DACULQ010000219 DACULQ010000000
VERSION     DACULQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.221"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            50..166
                     /gene="rrf"
                     /locus_tag="JA154_16855"
     rRNA            50..166
                     /gene="rrf"
                     /locus_tag="JA154_16855"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(380..1564)
                     /locus_tag="JA154_16860"
     CDS             complement(380..1564)
                     /locus_tag="JA154_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAT7997500.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1937..4288
                     /locus_tag="JA154_16865"
     CDS             1937..4288
                     /locus_tag="JA154_16865"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086576.1"
                     /note="Catalyzes the reduction of nucleoside
                     5'-triphosphates to 2'-deoxynucleoside 5'-triphosphates;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HAT7997501.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4310..4849
                     /locus_tag="JA154_16870"
     CDS             4310..4849
                     /locus_tag="JA154_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAT7997502.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAKEAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKQVFLVEQYMKDDLSIAE"
BASE COUNT         1951 a          598 c          825 g         1707 t
ORIGIN      
        1 ttctataaac ttattaaaaa cttaatagaa atattaagca taaagattat gtggttacaa
       61 tagcagagag gatacacctg ttcccattcc gaacacagaa gttaagctct ctagcgctga
      121 tggtacttgg tgggaaactg cctgggagag taggacgtag ccacgtaatt tttttgttat
      181 aataaattta gaatttaact tatatagttt aatttgataa tttgatgaaa aaaataaaag
      241 acttattgat ttttaaatct ataggtcttt tattttttat ctaacattta aattaagttg
      301 ttgaatatat cttatattac ttaccttata tattaattat cattaataca ttatatctaa
      361 ttactattca tctaaattat tatgccatta ttttatatac aaattctttt aatttatcta
      421 atccagataa gaaagtattt tcactgcaag cataggatat acgaatatat ttatccatac
      481 caaatgctat gccaggaaca actgcaacat tatattcttc taaaaattga ttacaaaatt
      541 ctatagaaaa actatcttta tattcaaatt tctcagatac tttagaaaga tctataaata
      601 tatagaatgc gccatttgga ttcacatatc ctacattatc aatagagtct agttttgatt
      661 taattaaatc tcttcttgat ttatatgttt taaccatatt gtctatgtca attgagcaat
      721 ctttcaaggc tccataggct atgtattgtg ctgttagact tgggtgagat ataagatgtc
      781 cttgtataga actcattgct ttagcaatcg ttttattaga tgcagtatat cctaatctta
      841 atccagtcat tgcagctgac ttagaaaaac catttatagt tattgttata tctttagctt
      901 cttcgcttaa tgatgcaatt gatgtaaatt caccagtgta gcatattttt tcatatattt
      961 catctgctaa aatatatatt ttattttgta tgcatacatc tactatttct attaattcat
     1021 cttttgtata aacactacca gtaggatttg aaggattgtt tattacaaga atttttgttt
     1081 tatctgttat agatttttca agttcttctt ttgttaactt aaatccattt tcttttttag
     1141 tatcaataaa aactggaaca gcattaacta acttaatcat ttcaggataa ctaacccaat
     1201 aaggctttgg taataagact tcatctcctt cgtctgttaa agctagtaaa gtatttgtta
     1261 ttgagttttt tgctccactt gatacaacta tttcgtctat tgaatagtta cagttatttt
     1321 cttcaataag ttttttacag atttcctctc taagtatttt taatcctgga actaaatcat
     1381 atttagtgta atctttattt aaagaatcaa taccataaga ttttgcgtta ttaggtacat
     1441 taaaatcggg ttctcctata ctaagattta ttactttaac tccattactt tccatttctt
     1501 taactttaga actaatacct attgtgtagg atggtgttat aaagtttagt ctttttgata
     1561 acataaaatg cctcctttta aattattatt tatattatac gacaaattta aagaatttac
     1621 ataaggaaat ttgtagatat atacattaat atatataata gcctacactt tttgcctaaa
     1681 attagtgtaa aaaaaagaga aaaaaatttt aactttcaaa gttgttagaa acgctactgt
     1741 tcaaaatatc cacatagtat ttgcaatgat ttgcgcaagt ttgactatta tgtacaaatt
     1801 tggtaatctt aatatagcaa ttattaagat aatcccttta acttagttga aggggatttt
     1861 tttagactta acatactaca tatagtggta tggggattaa taaaatacaa tatatagtaa
     1921 caggaggttt ttttaattgg ttgaattcgt aaaaaaaaga gatggaagag taattccatt
     1981 taatgaagat agaataacaa gagctatatt tttagcagca acgaatgttg cagaaagaga
     2041 aggaatagta cctgattata aattatctga gcaattaact caagaagtaa taaagttttt
     2101 aaatcataaa tactcagaat cagttccaag tgttgaagat atacaagatt ctgtagttaa
     2161 ggttcttata gaaactggcc atgcaaaaac tagtgaagaa tatataatat acagaactga
     2221 aagaagtaga ataagaaatt caaaaacaag gttgatgaag gcaatagagg aaattacctt
     2281 tgaagatgca gaagatgctg atataaaaag agaaaatgca aatattaatg gtaatacagc
     2341 aatgggaact atgttacaat atggaagcac tgtatctaaa gaattttgta aaacacatat
     2401 attaaaacca gagcattctt ttgctcatga taatggagat atacacatac atgatatgga
     2461 ttttttaaat atgggaacat taacttgttg tcaaatagat gttaaaaaac tattcaatgg
     2521 tggattttca acaggacatg gctttttaag agagccacaa gatataataa gttatggtgc
     2581 attagctgca attgctatac aaagcaatca aaacgatcaa catggaggtc aaagtatacc
     2641 atttttcgat tatggattgg ctgaaggtgt atataagaca tttaagaaat tctacatagg
     2701 aaatttagct aaagcattaa agttatttaa aggaattgaa aataatgatg tgataaagaa
     2761 catagtatat aatacagaga aagaaacaaa tcaaaaagtt ggtcttaaga gagatgaatt
     2821 gtatctaaac ttagaaaaag agaaattaat acagactttt gatatagatg atgaacttgt
     2881 aaataagatg caaaactttg catttgaaga atcgtataga gaaactgata aaaaaacata
     2941 tcaatctatg gaagcattta tacacaatct aaacacaatg cactctagag caggtgcaca
     3001 ggtaccattt tcaagtgtaa acttcggaac agatacttct gaagaaggaa gaatggtaac
     3061 taagaatctt ctattatctc aagaaagagg tttaggtaat ggagagacac caatttttcc
     3121 aattttaata tttaaggtca aagaaggcat aaatttaaat cctgaagacc ctaattatga
     3181 tttatttaaa ttatcatgca gagtatcagc taaaagacta ttccctaatt ttagtttttt
     3241 agatgcgcct tttaatgcaa aatattataa aaaaggtgaa ccagatacag aagctacata
     3301 tatgggttgt agaacaagag tacttagtaa tgtgtgtggt tcagaaacag ttagtggtag
     3361 aggaaacata tcttttacta cagtaaattt accaagattg ggtataaaac atggtattat
     3421 aaataatgaa aaggctaatt tagacggttt ctttgaagaa ttagatgaga agataaactt
     3481 aattatagaa caattattgg agcgccttga ggtacaagga aataaaaaga tgaagaattt
     3541 tccattctta atggggcaag gtgtatggaa aggttctgat gatttaggac cagaagatac
     3601 actaaaagaa gtaataaaac aaggaacatt gacaattgga tttataggtt tagcagagtg
     3661 ccttattgct cttataggta aacatcatgg agaaagtaaa gaagctcaag aattaggatt
     3721 aaaaatagtg tcacatatga gacataaaat ggatgaagca acggacaaat ataaattaaa
     3781 tttctcctta atgggtactc cagcagaagg tctttcaggt aggtttacta aaatagataa
     3841 aaaagtttat ggagaaatta aaggtatcac agataaagaa tattatacta attcattcca
     3901 tgtaccagtt tattacaata taagtgcata tgataaaatt gagatagaag ctccatacca
     3961 tgaattgact aatgcaggtc atataacata tgtagagtta gatggagatc catctgataa
     4021 tttagaagct tttgaaactg ttataaaagc tatgaaagat ttgggtatag gatatggaag
     4081 tataaatcat cctgtagata gagacccaat atgcggtttt tcaggtgtaa taacaagtaa
     4141 tatatgccct gtatgtggaa gaaatgaaga tgaaagtgat atcaaattcg aaagaataag
     4201 aagaataaca ggatatttag ttggtacagt tgatagattt aataatgcta aaaaagcaga
     4261 agtaagagat agagtaaaac atagatagtt ttatttgggg gaataagtta tgaaaataag
     4321 aatgtcatct actataagtt atgacagcat agttgatgga ccaggattaa gaatggtaat
     4381 atggacccaa ggatgtattc ataattgtaa agagtgccat aatcctcaga cacatgattt
     4441 atgtggagga ttttatatgg atacagaaga aattataaat aaagtcaaat cattgaaatt
     4501 acaaaaaggc attacgctat caggaggaga accattttta caaccagaac cattagaaga
     4561 aatagcaaaa gaagctaaaa taaatgggtt agatgtatgg tcatatactg gatttacgtt
     4621 tgaacaactg ttagataaaa aaaatagagc gtattttaaa aatttaaatt tactaaagca
     4681 aattgatatt ttagtggatg gaaaatttat agctgaaaaa aaagatataa gcttaaagtt
     4741 tagagggtca tcaaatcaaa ggataattga tgttcaaaaa agtttgaaat ataaacaagt
     4801 gtttttggtt gagcaatata tgaaggatga tttatctata gcagaataaa atataaataa
     4861 acaggattaa atatatgcat atatttaatc ctgtttattt tcatataata tttcaagctt
     4921 gtataatgta acaattaaac caaaagactc attgtatatt tgaaattaag ttttttatag
     4981 tttataggtg ttttatgtgg attacgaatg atatagtatt gatacattat ttaatagatt
     5041 cttatgccaa gaaacaatta atttatagaa attcttaatt g
//
LOCUS       DACULQ010000220         5065 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.222, whole genome shotgun sequence.
ACCESSION   DACULQ010000220 DACULQ010000000
VERSION     DACULQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.222"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(153..381)
                     /locus_tag="JA154_16875"
                     /pseudo
     CDS             complement(153..381)
                     /locus_tag="JA154_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            674..1315
                     /locus_tag="JA154_16880"
     CDS             674..1315
                     /locus_tag="JA154_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HAT7997503.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(1412..3076)
                     /locus_tag="JA154_16885"
     CDS             complement(1412..3076)
                     /locus_tag="JA154_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAT7997504.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(3148..4395)
                     /locus_tag="JA154_16890"
     CDS             complement(3148..4395)
                     /locus_tag="JA154_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAT7997505.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(4382..5056)
                     /locus_tag="JA154_16895"
     CDS             complement(4382..5056)
                     /locus_tag="JA154_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAT7997506.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
BASE COUNT         1621 a          908 c          536 g         2000 t
ORIGIN      
        1 ctataacaat tatacaatga tgtagaaact aaataaacag ccacatataa tatagcaata
       61 cttattatac attcaattga tataaaacca ctctttctta ttttcaataa taaaaacaac
      121 ccctaaataa tattttatat ataaacattt aactataagt tttttcgtaa atatcttttg
      181 taagtttttt cattgtaatc aaaatttctt cccatacata tataaattaa cacaccacct
      241 tcatccttaa cagtattaac tatgactgat acttttgctt gtaatcaact ttatacaaac
      301 aattaattac atttactttt ttaagtattg taaaattaca cctcctatat tcatattttt
      361 atagactcta atgctttgca ttaatctttt acttcaacac caaaacatat ataaatacta
      421 tttcctttta tatttattga cacctcaatt ccaatttata ctttataaat caataatttc
      481 ttcgccttgt ttcatatata tacaacaaaa acttaaattt aaattcccac tctttatgtc
      541 atattccatt caaaagtttg caaatatatg ttttaaattc taaaaatatg catattattt
      601 tattagataa cagtcaactt ggataagtct ccttcatata atttaaagtg accgttgaaa
      661 agggggtgac ctattggcag ctcttaaatc ttttgaaaaa cccttaaccc ctgaagaaga
      721 gattgagtat ttaactaaat ttaaaataga aaacgataaa agtgcaaaag atacattaat
      781 tgaaagaaac atgagattag tagcttatat agcaaagaaa tataataatt caacggaaga
      841 tcaggatgat ttaatatcca taggtacaat aggattaatt aaagctatag aaacttataa
      901 catagataaa ggaactcgat tagcaacata tgcttctaga tgtattgaaa atgagatttt
      961 aatgaatatt agatctaaca aaaaaaataa aactcaagta tctctacaag accctatcgg
     1021 aacagataaa gaaggtaatg aaataagtct tttagatata cttggtacag aggctaacta
     1081 tgttcttgat gaagttgaac taaaagttca agttggaaaa ctttacgaac aactaaacaa
     1141 aattcttact cctagagaaa gagaaattgt ccagttaaga tatgggttaa ctccttatgg
     1201 ctataagaca caaagagaaa ttgcacaaaa actcgatata tcaaggtcat atgtatctag
     1261 aatcgaaaaa aaagctctta aaaaactaga aaaagagctt gttcaagaaa gttaatacta
     1321 tattttaaaa ggatatatcg tacatcatta gacaaaattt tctatctaaa atcaaagtta
     1381 cgatatatcc ttaaatttta tagtacataa tttattttat actttcacat atttccttaa
     1441 atataggtgt tgcagattta tttccttttt gagttccttc tactaacaca gttattacat
     1501 acttaggtct ttcttcagga taaaatcctg ttatccatcc atggtctata gcttttttat
     1561 ttaaactact ttgagctgta ccagttttaa ctccacaacc accttcaata tcttttaggt
     1621 ctttagctgt accaatccta gatacagact tcatatattc tttaacttgt gtacacacat
     1681 aaggagaaat aagttcttct ttttttgaag acttataagt ttttattgta ttcatattat
     1741 tgtccacaag acttttatat aagtatagtg gtttgaatgt tccattatta gctattattt
     1801 gagtcatttg atttatttga agtggtgtaa attctatatt ttcttgacct atagctaaat
     1861 ttcttattga tatgtttttt ggagcttctc taattttttc ttcatcaaga ccaatatcaa
     1921 ccttttcaaa taaatgtaat ttttcagcag atttgagtat cttttctttt ccaagtttca
     1981 tagctatatc taaaaatgca ggattgcatg aattagaaaa agcttgtctt aagttttgga
     2041 aaccatgacc atctctttta ttacatcgca gtatttcatt agtattccca actttagtct
     2101 tacctgtaca gttgtaagta tagttctcat ctataacacc attttcaagt gcagaaaata
     2161 aaactaccat cttaaataca gaccctggtg gatatgtcgc ttttataact ctattttcaa
     2221 attctccatt ttttcctttt aaactttttg atatgtcatt ttggtcaaaa ttaggtctag
     2281 agcacattgc aagaatttca cctgtagatg cttccgagat tattgctgct gtaggatttt
     2341 cttctttatt tagtatttgt tctaattttt tttgaatgtt ataatcaata gtagttttta
     2401 gatgtctatc tttatcctta ttatcaacag tttttacact tccctttagt atgtttagag
     2461 atttttcatt tcctgcatct ccagctttaa aagcacttat atacttttca tttgaatttc
     2521 taagtacact atccatactc ttttcaattc ctgattgacc tgatttatca tcttctttta
     2581 tatatcctat tgtatgtgat aataaaccat cttttgcgta cctcatagtt ttttcttcta
     2641 ccattattcc atttttctca agttcttttt tcatactctt atcgatattt ttagtctgaa
     2701 tctgtattat agggcgtgtc aattgctctt gtacagcttt atatatatct aaatcattca
     2761 tcttagtagc tttcttaata agctccaaaa ttttatagtt attatttaat ttttcttttt
     2821 caacaattaa tacctgagat ttacttgtat ctgtaagctt cttgttgttt ctatcataga
     2881 ttattcctct tccactattt aactcgactt tctcaacgct ttgtgattca acactttgtt
     2941 tgtatttatc accttttaat acttgtatat caactagcct ataaattaaa acactataaa
     3001 ttattaaaat tatagtaaat atacaccagc ttctctttcc cacttttttc aaaaatggag
     3061 tcttcttttt tgacatgaaa tcacccctta gataatttca ttattgacaa tttctaaggg
     3121 gctaatacta tttttatcaa ttttttatta ttcttcaacc ttttcttcta taacttttct
     3181 aagcatataa tctttaccaa cttttactga tagctttaac ttcactattt gtctaggatg
     3241 aggagctgat tctatagctt ctccttcttc attgtacatt tcttcaattt tagtaaacat
     3301 agtttcttta tatggtccca taacttctat ttcatcacca acacacattt tatttctttg
     3361 ctcaacaaca ataagaccat cttcatcttc tgtttctcta actataccta taaaatcata
     3421 gtttctaaca tatgatgctg attgataatt ttggtcttct gttgttggtt tattcaagta
     3481 aaaacctgat gtaaaatgtc tatgactacc tttttttagt tcttccatcc acattggatt
     3541 gaacttccag ttttctgggt ctctgtaaaa ttcatctatt gccattctgt atgctcttac
     3601 aacagtagct acatagtatg cagtcttcat tctaccttct atttttaaac ttgttatacc
     3661 agatttgatt aactctggta tatactctat catacataaa tcttttgagt taaagaagaa
     3721 tgtacccctt tcatcttcgt atattggaaa atactcacca ggtctttttt cttccaccaa
     3781 atgatattgc catctacaag attgagcaca agaacctttg tttgcatctc ttccagtcat
     3841 atagttgctt attaaacatc tgccagaata agaaatacac atagcaccat gcataaacgc
     3901 ttctatatcc atatctaaag gtgtttttgc tcttatctca gatatttcat caaaagataa
     3961 ttctcttgcc actacaactc tttttattcc atgctcgtac cagaagtttg cacttagata
     4021 attggttgta tttgcttgtg tacttaaatg tatttccata ttcggaatta cttttttaat
     4081 gacactaagc actcctgggt cagctattat aactgcatct attcctatct cttctagttc
     4141 caacaaatag tcttctaatt gtagaaaatc ttcattatga ggtattatat ttactgttac
     4201 gaatactttc ttgcctcttt catgtgcaaa agctactccc tctgccatat cttccttact
     4261 aaaattcttc gctgcagacc tcattccaaa aatttctcca cctatataaa cggcatctgc
     4321 tccatatgtt atagctattt tcaatctctc caaatcacca gctggagcta aaagttctac
     4381 tttatttaat tccatctaca tcacctcttt tactttcttt ataacttagt gctacaccat
     4441 cacctattgg taccaatgat gtgcttaaat agtcacaatt acatatataa tctaaataat
     4501 ttctcattct tttaactata gtcttttttc ttcttacaac aaaatcatca tgagcaacca
     4561 tacctttata caaaatatta tcagatacta aaagtccccc atcttttaat ttatctataa
     4621 ctaaatcaaa aaatagctta tattgacctt tggctgcatc tataaatata aggtcaaatt
     4681 caccttggac ttgactaagt ttttcttctg catctccttc tagtatagtt atgttattgt
     4741 caaatccagc taattttatg ttttcttttg ctttttctat cattttttca tttctctcaa
     4801 cagttattat atctgcatct tttcctataa tagttgcaaa aaaaatagaa gaataaccaa
     4861 ttgcacatcc tacttctaaa attctctttg gtttttgaac ctttaacaag acttttaata
     4921 aatctgaaac ttctttatgt attataggaa cactattttc ctctgcataa atttctaaat
     4981 cttttaaaaa tccttctttt tcttttaatg tcactcttat ataatcttca accaaatcat
     5041 taactatatt actcataaaa acact
//
LOCUS       DACULQ010000221         5021 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.223, whole genome shotgun sequence.
ACCESSION   DACULQ010000221 DACULQ010000000
VERSION     DACULQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.223"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            473..1333
                     /locus_tag="JA154_16900"
     CDS             473..1333
                     /locus_tag="JA154_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter"
                     /protein_id="HAT7997507.1"
                     /translation="MKIIQILNNNVTLVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKASDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1323..3194
                     /locus_tag="JA154_16905"
     CDS             1323..3194
                     /locus_tag="JA154_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit EIIBCA"
                     /protein_id="HAT7997508.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3214..4668
                     /locus_tag="JA154_16910"
     CDS             3214..4668
                     /locus_tag="JA154_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAT7997509.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKIGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         1944 a          523 c          906 g         1648 t
ORIGIN      
        1 aatttgaagc tttacctatt aatatatctt aatagtgatt aaataaaact aaacttaaga
       61 ctttatatgt taatatatct taagaattta tttgttaaca tacagttcat tatatttaaa
      121 agcattacta tgataattca taatagtaaa atagataatg gttcagacaa tattataaat
      181 attagtgacg gtaaaatagt atcaatttag tatataaacc ttgtcagata atcgcataac
      241 agagcttgta gttatagaat aaaaaaatat ctaggaaacc gttgacagat ttatatggat
      301 agtataacat atagaatatg aaattaaaca aaaaagaatt ttaattatgg cgacgtagtc
      361 ataattttga gtatgttact gattagcagg cagaactcaa gtatatatgt gaaaaataga
      421 gggatactct atacatatat gcttgagttc tttttattga aaggaggaat atatgaaaat
      481 aattcagatt ttaaataata atgtaacctt agtaaaaaga ggtaaaaatg aaattattgt
      541 atttgttaaa ggaattggct ttaaaaaaag agtgggacaa gtaataaatg aaaatgaaat
      601 agagagaaca tatatactag attcatacga tatgctagaa cactttagtt atctactaca
      661 aaactcagac tccaataata tagttcttat taatgaaata attgaatatg gtgagaaagc
      721 tttaaatata aaggcaagtg attatctaag cttaacctta ctagaccatt tagagttttt
      781 acttaaaaga tgtgaaaaga atcaatttat aaaaagtcct ttggtatgga atgttaaaag
      841 attttatcca aagcattttg aaattggaat gcatgcacta aaattaattg gtgatagtaa
      901 gggaattaat cttcctgatg atgaggcagt atcaatagca cttcattttg taaatatgga
      961 ggtaaataaa gagtctcatg atagtacaat tgtagagttg agaactttag cagatatagt
     1021 ttctataata aaatatcatt ttaatgtaga gctagatgaa acttcaacaa attatatgag
     1081 atttactact catctacagt actttatcca aagaattatg tcggatgaag cttcaataga
     1141 tgaagataat tctatagatt tatataaaca agtaagtcaa ctttatccaa agtcatttgg
     1201 aagtgttcaa aagataagaa aatatataaa atcacaattt aatgtcgata tatctataag
     1261 tgaagaaact tatttaatgt tgcatataaa tagggtgact caaagactgg aggcaaaatc
     1321 agatgaaata taaagattta aatgaaaata taataaaact tataggggga aaagaaaata
     1381 taaagtcggt agcgcattgt gttactcgtt taagatttac tttaaatgat aggagtatcg
     1441 ctcaaacaga agaaattaaa aaattaaaag gagtaattga tgtagtttct aatgatgttg
     1501 cttatcaaat tataataggt actcatgttg tagatgtata taacgagttt atgtctatga
     1561 taggtttaag ttcaagtgaa tcaagtataa aaagtgaaag aaaaaaggga attagaggta
     1621 tcaaaggtat atttaattcc atctttgtgg tagtctcaga gactatgact tcagtaattg
     1681 aagtactact tgcagctggt atacttgcaa gtatattagc acttttaaat atgtctggca
     1741 tacttcagag tgacagtcct acatatcaaa tattagatac attaagaagt gctacatttc
     1801 acttcttacc agtattaatt gcaatagcat cagcaaaaag acttaatgta aatccctata
     1861 tagcagtagt gttagcagtt acgttattat cttcatcaat agatggtgta gaaggattaa
     1921 gtttatttgg tataaattta caggctacaa cttatgcaaa cagttttatt ccaatacttt
     1981 taggagtatg ggcaatggga attataattg aaggtttgaa aaaaatatta cctaaagcat
     2041 tacactattt tttagttcca gtgttatctt taataataac tctatcagtt acattaactg
     2101 tatttggacc tattggtatg tggattggtg atggattaaa ctatgcatgt atgtttttaa
     2161 ttgatacatt gggtaattgg agtgttgttg cactgtatgc agctttacaa ccactcttaa
     2221 tagtttttgg tgcagcaaac tttactatgc ctatatcttt aaattctgtg acaacattgg
     2281 ggtatgaccc aatttttatg ggcgctgcaa caatatctga tattgcagtt tgtggtgcaa
     2341 tgtttggata tttcttaaaa actaggaata aagaacaaaa acaactattt ggtacagtaa
     2401 gttttagtgc acttatggga gttacagaac cttcagtgtt tggtgtgttt atgaaatata
     2461 gaagaccatt tgtagcagta atgataggtg gaggtatagg tggtttaata gcaggtttag
     2521 ctcaagttaa aacatatgga atggtttggg gattagctgc attaccaacg tatcttgttg
     2581 gaggagaggt atctaacttt acatttatga taataagtgt agtagtatca tttgtagcag
     2641 caacagcagc ttcctattta ctaggaatac cttcagatga aaaagaagaa gttaagggtg
     2701 ataatgaaaa agaattagaa ctaataatca acgagaacaa ttcaagaact attagtattg
     2761 gaaatgttgc aaaaggtcag gtaattgcac ttggggaagt taatgacaaa gcattttcct
     2821 ctggagcatt aggtaaaggg gttggagtta tacctagtga atcaaaatca actgttatat
     2881 ctcctgtaga tggaactata actatagtat ttccaactaa acatgcatat ggtatagtga
     2941 ctgatgaagg attagaaata ttaattcata ttgggataga tactgtaaac ttagatggaa
     3001 aattctttga aagtcttgta acacaaaatc aaagggttaa aaaaggagat gctttagcag
     3061 tatttgaatc tgataaaatt atagaggaag gctttgattc tactataata actgtagtta
     3121 caaatactag cgattattta gatgtaatct catcaaatga tgaatcagaa gaattattaa
     3181 ctgtaatagt ataaatttta ggagggaaaa ttcatgaata ttaaagatac atttttctgg
     3241 ggaggaagta ttgcagccca tcaatgtgaa ggttcatggg atagtgataa caaaggacct
     3301 gcaattatgg actttgtaac taagggctct tatgaaacgc caagagtaat tactgataaa
     3361 atagaagaaa aattagatta tccatctcat aatggaatag atttttataa tagatataaa
     3421 gaagatatag ctttatttaa agaaatggga tttagtgcat taagaatatc tatagattgg
     3481 tcaagaattt ttccaaatgg agatgatgaa aatcctaatg aattagggat taaatactat
     3541 gaaggtctga tagatacatt aatagaaaat aatatagaac ctattgtaac actatatcat
     3601 tttgaattac ctatgaattt agtacataaa tatggttcat ggaataacag aaaacttata
     3661 gatttgtatt taaagtacag tgagactgta ataagaagat ttgatgataa ggtaaagtac
     3721 tgggttacat ttaatgagat gaaccatatt gaccctcaaa cagaacactc ggatattttt
     3781 acttatatga tagcaggtat taagtatagt gaaatagaaa ataaggttca aacattggca
     3841 aatgtagggt ataatatgac tttggcgggt gtaaaggttg caaagttggc tagagaaatc
     3901 aatcccaaca ataaaattgg atgtgtattt ggactaaatc caatttatcc atacaattgt
     3961 aatcctagta atgtgttaaa tgcattttta gataatgacc gtgattatta tcaaattgat
     4021 gcaatgtgca atgggaagtt tccacaatat aaattaaaag aatatgaaaa aaataatata
     4081 actattgaag tgactgatga agacaaaaaa gcatttaaag aaggtaagtt agattttata
     4141 ggacttaatt attatttttc aagtgtatct actccaaaag aaaatttatc aggtgataaa
     4201 tctttatttg gtgggattca aaatccatat ttggaacaaa gtaagtgggg gtgggcaata
     4261 gaccctatag gtttaagatt taccttgaac tatatttaca gaaagtatca attacctatc
     4321 ttaattacag aaaatggttt aggggcatat gataaaatcg aagaagatgg aactataaat
     4381 gatgattata gaattgaata tttaagtaag catctatctc aaatgaagtc tgcaattgaa
     4441 gaagataatg ttgaatgttt tggatatttg atgtggggac caattgattt agtcagtgca
     4501 actacaggtc agatgagtaa gagatatgga tttatctatg ttgacttaga tgatgaaggc
     4561 aaaggaagtt ttaagagata taagaaaaaa tcatttgatt ggtacaaaga agtaataaaa
     4621 tctaatggag aagttttaga tttttctacg gatatagtag tagggtagga ataagataaa
     4681 tagaagttag gaactaatgt taattagtgt ctagtaaagc atttaaaata gagtataggg
     4741 tctttaaagt aaagtgtgta ttcttcttaa aaataatggc aattaaaatt actataaaaa
     4801 ggagaataca cattttttat ttttatactt aagtaaatat ttagacatat tcatagtata
     4861 atttactcat tatataaaaa tagtatgatt tactcactat ataactttat aaattttctg
     4921 gaaaaatgtc agaatatatt gacaaagaaa agatttaaaa ttataatcta tattaagaat
     4981 agtttgacaa tataaaaaca agtattttta taaatagtga a
//
LOCUS       DACULQ010000222         5003 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.224, whole genome shotgun sequence.
ACCESSION   DACULQ010000222 DACULQ010000000
VERSION     DACULQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.224"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1606
                     /locus_tag="JA154_16915"
     CDS             <1..1606
                     /locus_tag="JA154_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997510.1"
                     /translation="TFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKH
                     IETMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLI
                     VVLSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYA
                     YKEIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFII
                     IQGILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKED
                     SIEHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKT
                     TLLKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGD
                     VYKSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMK
                     DADMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENG
                     KLLESGRHDELMDKKGVYFNLFCNCK"
     gene            1628..3049
                     /locus_tag="JA154_16920"
     CDS             1628..3049
                     /locus_tag="JA154_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HAT7997511.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3078..4148
                     /locus_tag="JA154_16925"
     CDS             3078..4148
                     /locus_tag="JA154_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HAT7997512.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4164..4415
                     /locus_tag="JA154_16930"
     CDS             4164..4415
                     /locus_tag="JA154_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HAT7997513.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4466..4669
                     /locus_tag="JA154_16935"
     CDS             4466..4669
                     /locus_tag="JA154_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997514.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2054 a          440 c          802 g         1707 t
ORIGIN      
        1 aacattttcg aaaagtgtaa aagaaatatt agttttgtta attatgtatt taatcataaa
       61 tataattcct tcaatttttc aaagttttta tggatattat agaataaagt ttaataaaaa
      121 ttttgaaaaa catattgaaa caatgatgtt agagaaatct ttaactttga gtttaaaaga
      181 ttttgaggat gataatactt atgacatgat aaatagagct caatctcaaa aaggatttaa
      241 tattactata ctatctgaaa accttatttc aataataaga gaatcagtat ctacactttc
      301 tcttattgtt gtattatcac aatttcaact ttggctaata atagttgtta ttgttattcc
      361 tgtattgaaa tcattatatt ccattaaatt aggaaaggaa caatatgatg ttagtgtaaa
      421 cagaacttgg aaagaaagaa aggcctggta catagattat ttgttgtcaa aaggttatgc
      481 ttacaaagag attatggtat ttagaattgg ttctaaactt attaatcatt ataaaaaatt
      541 aaaagaggat attatatctc aagatatgaa tatagctaaa aagaatttta ttgctacgct
      601 attatgtaca gttttagatt caattgtatc tgcaggaatt ataggattta taataattca
      661 aggtatttta aagaagattt taataggaga tgcgactaca tatatacaag cagttcaatc
      721 tataaaggat aatgttgaat caatattttc gttatttagt tcagtaatac aagatctttt
      781 ttatacagat ttactgttta aatttctaga ttataactgt aaagaagact caattgaaca
      841 tataaaaata tttgaaaaaa taggggatat aaataaaata gagttaataa atatttcata
      901 taaatacaat cattcatcag agtattcttt aaaaaatatt aatttaactt taaataaaaa
      961 tgatcccatt gctatactag gaaaaaatgg ttcaggtaaa acgacacttt taaaaatatt
     1021 attaggtttt tatgatgatt atgaaggtga catcttgata aatggaaaaa atttaaagga
     1081 tattgaaaaa gatgaataca ttagaaaagt aagttgtatg tttcaagatt atattaaata
     1141 tgaggctaca ttaaaagaaa atgtcgccat gggagatgta tataagagta acttggaaga
     1201 aaaagttaaa agtaaactta aagaaactaa attgaaacca agtatttatg aggaagatgg
     1261 attagaaact attttaggaa cctggtttgg taagaagcaa ctttctatgg gtgaatggca
     1321 aaaagtagca atatctagaa ctcttatgaa agatgctgat atgtacattt ttgatgaacc
     1381 agattcatct ttagattctg aagcgggaaa tgaactcctt tctttatata aaaaaacatt
     1441 tgaaaataag attggaatat ttataacgca ccatatccaa catgcaaaaa agataactaa
     1501 caatattgta gttcttgaaa atggaaagtt gttagaaagt ggaagacatg atgaacttat
     1561 ggacaaaaag ggtgtttatt ttaatttatt ttgtaactgc aaataaatat taaagggagg
     1621 ttaattaatg aatcaaaaac catttataca cacatttaaa gctggaaaat catattttat
     1681 ttatgatgtt aatacggata agattttaaa ggtaaatgct gctgtttata attatctaaa
     1741 taaaattgag cataatttag aaaaagaaag tgactggaac atagaaatag aagacgaaat
     1801 taataccttg attaattatg gatttctaaa aaataaaagg gtaagtaaaa caatgcaccc
     1861 tgaaactaag tatataaaat atcatataga aaataatcta tcctctatta ttttacaagt
     1921 aacccaaaat tgtaatttac gttgtgacta ttgcatttat tctggttcat acagcaacag
     1981 agttcataca aataaaagaa tgaatataga tgtagcaaaa agaagtattg actatttgat
     2041 taagaattct agtcaattaa atgaagttag tgttggcttt tatggaggag aacccttact
     2101 tgagtttgat ttgattaagc agatagtaga atatgtaaaa gtagttggag aaggaaagga
     2161 aatatacttt aatctaacta caaatgcaac tttatttaat gaggaaataa tagaattctt
     2221 tgtaaaaaat aatataacaa ctatgattag tttagatgga ggaaaagaaa ttcatgataa
     2281 aagtagaaaa tttgcaaata caggagaagg atctcatgat gttgtattaa aaaatgtacg
     2341 ttacataaaa aataaatatc cagaatatta taaaaagaat attacattta atacagtgat
     2401 aaattcagaa aatgattaca tttctataga tgattttata tctaaaaatg agactttaaa
     2461 agattcaaac tttttagctt ccactcttaa taataactat tctgataaag aaatagtttc
     2521 atcagatgaa tatatattag gtaagagtta tgatagattc ttgactttac tcaacaaatt
     2581 aaaatttata aaagatgaga gtatgtcacc tttatcaaga atagaaagag ccttattaga
     2641 tgattttaga tatgagaaaa atattgaagg tagattagag ttaccagatg tgtggcatca
     2701 tggtggacca tgtataccag gagcttttag aatatttgta actacagatg gagaattttt
     2761 tccatgtgag agagtaagtg aaagttctaa aatgggtatg attggaaatt tagatagtgg
     2821 agtagatata gaaaaggctg aaaaactttt aaatatagga aaattatatg aagagcaatg
     2881 ttctgattgt tgggcctata gttattgtga tatatgtata gctaagattg atgatactaa
     2941 atgtaatgaa aatgaaataa aaccacatga ttgtgacaga attagacatt ctacagaaaa
     3001 taatatgaaa gattactgcg ttttgaaatc tctaggatat caatattaaa ttttctaatg
     3061 aaaggaaaga tgggattatg gaaaaagtac ttatatatcc atttaattat aattttttta
     3121 ctattttaaa tcattgcaat aaaatttcag atatagaaat taattcactg gttagtcctt
     3181 ctggctgggg gttatgcaat aaggagtata atgttaataa tattattact aaagttacaa
     3241 atgattttca aagtgaactt aaaaagaatt ctttggtatg gtttgttgat tcaaaagaaa
     3301 atctagactt agaaaaattt ataattccta aattaaaagt agctctaaaa gaaggtaaaa
     3361 agatattata tacacgtgat gatcttaata aaatatctag gtatatatct cattatgaat
     3421 tcaaaaagat taatgaatgg aatttagaaa aatatgaaat aagagatata gagggtatat
     3481 cacagataaa cacaccaata atatttattt gtggtcttta tggagggtta gatacttttg
     3541 atgtaaatat aaggatagga gaagaactaa gaaaaagggg gttttcaata ttacaatttg
     3601 gccctaaaaa agagagctat ttatttaaaa tgattcctat accagagttt atgtttgatg
     3661 attcgataag tagtaagaca aagattatta attttaacca ttttataaaa gataaagagg
     3721 tagaggcaaa gccagattta ataattatag aagttccagg agaattattc cctttctcta
     3781 gaaaaatggt tggtaatttt ggaattagag cttttgaaat ttctaatgca gtaataaatg
     3841 attgtggtat attatgtatt ccatcaagta tatatgatag agaatattgt gaagaactta
     3901 aatattttat taaaggaaga catggtataa atatagacta ttttagtata gagaaaaaag
     3961 ttatagatac atatgagaca gaggtgaagc aaaaattttc atatatgtca ttagatgaaa
     4021 acttttttgt tgaagatata gaagattatg aaaaaacatt ttttatacat gataataaac
     4081 aattactaga attagtggac aatatagtga atcaattaaa agagtatgct aaagtaatat
     4141 ctatttagaa ttggaggtta attatgaatt acactgaaaa ggaattacta ctagagaata
     4201 tattttttga tgtgactgga attaatttta ataaaaataa caatttaaaa aaagaattat
     4261 ttttttcacc gtctttacat atacagccaa gagaattact tttagtattt tgcgaggtaa
     4321 tagaaaactt taatataaaa attccagagg aaaaactttt gaataggcaa tttacgagtt
     4381 ttgataatgt tctaaaaata atcaatatgg tataggttaa taaaggtata aagtaagtga
     4441 tcttatagaa aaggggagga tatgaatgag ttttggaaaa agaatagaaa tgaataatat
     4501 aggtgttgag acaagaggtg tttgtactat tatgtgtcct tatacttgta agatgatggc
     4561 atcttcatct tgtaactgca agaataatga ttataatgca agaggaggat catataatac
     4621 atattataat aaattgatac atggccaaag aactcctgat ggaatgtaaa tatttcagta
     4681 ataagtatac tattaaattg atttagcata tttgattaaa tttatctttc ttactttatt
     4741 tccttttatt ataaaaatat gcagttgatt ataattaaga gtgtgatttt ataataactg
     4801 cataaatagt atctgttata taaatcacct cttcaattat tatagtggta aatatagtga
     4861 ttaggggtga tttttacaat ttaaaagtat aaattaatta aaagttatta taataaatta
     4921 tacttattta aatagtaaga attaaaagta aagtttttaa atatatttgt aaaaatgtat
     4981 atatatatta ataatattga tta
//
LOCUS       DACULQ010000223         4936 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.225, whole genome shotgun sequence.
ACCESSION   DACULQ010000223 DACULQ010000000
VERSION     DACULQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.225"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..217)
                     /locus_tag="JA154_16940"
     CDS             complement(<1..217)
                     /locus_tag="JA154_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAT7997515.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELIFL"
     gene            444..2855
                     /locus_tag="JA154_16945"
     CDS             444..2855
                     /locus_tag="JA154_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HAT7997516.1"
                     /translation="MRKYKSKNLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            3152..4729
                     /locus_tag="JA154_16950"
     CDS             3152..4729
                     /locus_tag="JA154_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HAT7997517.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         2016 a          595 c          869 g         1456 t
ORIGIN      
        1 acaaaaatat tagttcaatt cttttactct caaacacaaa aaacttatta gtaacataag
       61 caaataaaac agcaagtatc catgccaaag catttgcaac cataaaatta aataaaataa
      121 ctctagtaaa aaataaatac gaaactatat ttacaagagt agtaaatgct ccaaaaaata
      181 aatataatat cgtctctcta tgctttttta aaatcaaata taatcctcct aagctaattt
      241 aatataaata tacttaaaga tactaacaaa cattagtata acacatctat taataaatca
      301 atatatctta aaatattata aaaataaaac tataatattc tataattaat atgtactcat
      361 aatccatatt ttatcaaaat aaaatatgac cttaatttag aaatctgata taataaaaat
      421 taaatacata taagggggta aacatgagaa agtacaaatc aaaaaacttg tcaaagctac
      481 tagcattgtt gacagtttgc tttttaatag tgtcaacaat acctgtctca gcagaaaacc
      541 ataaaactct agatggagta gaaactgcag agtattctga aagctatctt caatacctag
      601 aagatgtcaa aaatggagac acagcaaaat ataatggagt aatacctttt ccacatgaaa
      661 tggaaggtac aacacttcga aataagggta gaagtagtct tccatcagca tataaatcaa
      721 gtgtagcata caacccaatg aatcttggtc ttactacacc agcaaaaaat caaggaagtc
      781 ttaatacatg ctggtctttt tcaggtatgt caacgttaga agcatatctt aaattaaaag
      841 gatatggaac gtatgactta tcagaagaac atttaagatg gtgggcaact ggtggaaaat
      901 atggatggaa cttagatgat atgtcaggga gttcaaatgt aacagctata ggatatctga
      961 cagcatgggc aggtcctaag ttagaaaagg acataccata taatcttaaa tctgaggcac
     1021 aaggtgcaac taaaccttca aatatggata ctgcacctac tcaatttaat gtaacagatg
     1081 ttgttcgtct taataaagat aaggaaactg taaagaatgc tataatgcaa tatggttcag
     1141 tgacatcagg ttatgcacat tattcaacat attttaataa agatgaaaca gcatataact
     1201 gtacaaataa gagagctcca ttaaatcacg ctgtagcgat agtaggatgg gatgataatt
     1261 attcaaaaga taattttgca tctgatgtaa aaccagaatc aaatggagca tggttagtaa
     1321 aaagtagttg gggagagttt aattctatga aaggattctt ctggatttct tatgaagata
     1381 aaactctact aacagataca gataactatg caatgaaatc agtatcaaaa ccagatagtg
     1441 ataaaaaaat gtaccaactt gaatatgctg gtcttagcaa gataatgtca aataaagtaa
     1501 cagcagcaaa tgtatttgat tttagcagag actctgaaaa acttgattct gttatgtttg
     1561 aaacagactc tgtaggagca aaatatgaag tatattatgc accagtggta aatggagttc
     1621 ctcaaaacaa ttcaatgaca aaacttgcaa gtggaacagt atcatattct ggatacataa
     1681 atgtacctac taattcttac agcttaccaa aaggtaaagg agcaatagta gtagttatag
     1741 acaacacagc aaatcctaat agagaaaaat caactttagc atatgaaact aatatagatg
     1801 catattattt atatgaggct aaagcaaact taggtgaaag ctacatactt caaaacaata
     1861 agtttgagga tataaataca tatagtgaat tttctccttg taactttgtt ataaaagcta
     1921 taacaaaaac atcttctgga caagctactt caggagaatc tttaactgga gcagatagat
     1981 atgaaacagc agttaaagtt agtcaaaaag gatggacttc ttcacaaaat gcagtattag
     2041 taaatggaga tgcaatagtg gatgctttaa cagctacacc atttacagca gcaattgact
     2101 ctccaattct tttgacagga aaagataact tagattcaaa aactaaggca gagttacaaa
     2161 gattaggaac taaaaaagtt tatctaatag gtggagaaaa ttctttaagc aagaatgtac
     2221 aaactcaact tagtaatatg ggtatatcag tagaaagaat ttcaggtagt gatagataca
     2281 agaccagtat atctctagct caaaagttaa atagtataaa atctgtttca caagttgcag
     2341 tggcaaatgg tgtaaatgga cttgcagatg caataagtgt tggtgcagca gctgctgata
     2401 acaatatgcc aataatactt actaatgaaa aaagtgagtt gcaaggtgct gatgagtttt
     2461 taaattcatc taaaataact aagtcttata taattggtgg aacagctact ttatcatcaa
     2521 atttagaaag taagctttca aatccaacaa gacttgcagg cagtaataga aatgaaacta
     2581 atgctaagat aatagataaa ttctacccta gctcagattt gaaatatgct tttgttgtta
     2641 aagatggttc aaagagtcaa ggagacttga tagatggact agcagtagga gcattgggtg
     2701 ctaaaactga ttcaccagta gttctagttg gaaataagtt agatgaaagt caaaaaaatg
     2761 tacttaagtc taagaaaata gaaactccta ttagagttgg tggaaatgga aatgaaaatg
     2821 cttttaatga actaaatact cttttaggaa aatagtgaat ggaaagatag taaggatata
     2881 cattttatta ttaaaaaatg ttcaatagaa gtttataaat ataaaataac aaaaaggggt
     2941 gtctcaaaat gaacttttta gttcatgagg cactcttttt tgttgtttta tatttttatc
     3001 atttaaataa tgaaaaatag gattgtctat atttttagat agttttctta ttattgatga
     3061 aaaatgtaat aattttaaac tgtaaaatag tgtaaagtta ttttataata taaatatagt
     3121 aagattttaa acaaaataag ggggaataat aatgaaagca ccaaaaacta ttttaacaat
     3181 actgacaata gcgcttactt taagtagcat ttctataata ccatcatatg cacttacaga
     3241 ggaaaaatta ataggtaatg gtagatatga aactgctgta aagataagtc aaaaggcata
     3301 cagttctagt cctaatgtag tgctagttaa tgacaattca ttagctgatg cactttcagc
     3361 tacaccattt gcaaaggcta aaggagcacc aatacttttg acagaaagtg ataagctaga
     3421 tgataggact gaaaaagaga taaaacgtct aggagctaaa gatatttatt tgataggagg
     3481 tactgctgta cttaataaag atatagagaa caagttaaaa agtaatggac ttaatgtaga
     3541 aagaattaat ggtaaggata gatatgaaac ttcactgatt ttggctaata agcttaagga
     3601 tataaaagat attaaggaag ttgctgtagt aaatggtgaa aaaggattga gtgatgctgt
     3661 tagcgttggg gcaccagcag ctcaaaataa gatgcctata attttgtcta gtcctaagga
     3721 tggagtagaa gcatttgata aatttataag ggatgagaaa gttataaaag catatgtaat
     3781 tggaggtaca aattctgttt caagagctgt agaaaagagt cttcctaatg cagaaagagt
     3841 gagtgggaaa gatagaaatg agactaatgc taaagttata gaaaagtttt acactgatac
     3901 aaatttaagt aatttatatg taactaagga tggtagtaaa aatgagaatc aactaataga
     3961 ctcgttagct gttggcgtat tggctgcaaa aaatgagtct cctgttgttt tggttggtaa
     4021 taaattaaac acaaaacaaa gagatatact tagtactaaa aaattaaata ctataacaca
     4081 ggtaggtgga aatggaaatg aagaagcttt tgatgaaatt aaaagtctac aagaaaaaac
     4141 tgtgtttgaa gcaaaaactg ttgaggaatt aactgatatg ataaatatag caagtcctaa
     4201 tgatattata aactttaaac caaaagagaa tactgtaaat gaagctttta gaatggtaac
     4261 aaataaacca ataactgtga atataaaggg tgattgttca aagactctta cagtagatat
     4321 gcctaatggt gaagtgaata actatgctac tttggtaaat gttatagtta gaaatattgg
     4381 agaaggtgga tttaataatc atgatacaat cactatattg tcagttcgtg acaaaaatgg
     4441 tagagttata gaaaatacta gaaattcaga tatagatacg ttgatgatat tagcttctgc
     4501 taatgataca aaattgatta atgatggata tataggaaaa cttatagata attcatctaa
     4561 tagtgatata actaataatg gtactataga taaaaaagtt aatcaggttg aagatttaga
     4621 ggctaaagta gattcaattg aaaaagctat agattctata agtcaaaaag tcaataaaat
     4681 acaagatata ctagataagc ttggatttct aaagaaattt cttagctaag aatatgtata
     4741 tttaagtgaa gtgtgtgttg taattgttct aaagttatgt agttaaacag gtttatatac
     4801 aaatagattt atagataaat atgtagttta gtatatattt gaataagctt tggggaggta
     4861 acatggggat tggaattgaa tgtaacaaaa aaataaataa aagtattgac aaaactagaa
     4921 taaagaaaaa taatct
//
LOCUS       DACULQ010000224         4850 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.226, whole genome shotgun sequence.
ACCESSION   DACULQ010000224 DACULQ010000000
VERSION     DACULQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.226"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            368..1381
                     /locus_tag="JA154_16955"
     CDS             368..1381
                     /locus_tag="JA154_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAT7997518.1"
                     /translation="MNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYIFISDKF
                     DDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNIRLINLS
                     LSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFTLDGDDS
                     FWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEKPGITIS
                     DLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCLDIEENN
                     EKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLTELVERY
                     KNL"
     gene            1514..2812
                     /locus_tag="JA154_16960"
     CDS             1514..2812
                     /locus_tag="JA154_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAT7997519.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPSNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(2926..3171)
                     /locus_tag="JA154_16965"
     CDS             complement(2926..3171)
                     /locus_tag="JA154_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HAT7997520.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3319..4185
                     /locus_tag="JA154_16970"
     CDS             3319..4185
                     /locus_tag="JA154_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HAT7997521.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4189..4725)
                     /locus_tag="JA154_16975"
     CDS             complement(4189..4725)
                     /locus_tag="JA154_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HAT7997522.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
BASE COUNT         1978 a          493 c          768 g         1611 t
ORIGIN      
        1 attgttctcc ctaaataaga tttagatgta tagtattaaa atcaaaaagt ataaaataaa
       61 attgatgctt aatcatagat aaatattaat aaaatataca aggggattgt ctaataaaaa
      121 gataatctcc ttttttacaa ataattctaa attgtgatat aaatctaaaa attttatctt
      181 aacatttaac catttaatga taataattga tataataaat ataaattaaa tatatggtgt
      241 acttatttta tgtttttagc aagaaatata gcatttaaaa gataaattgt gcaacttaaa
      301 tcaaagttta agatgaacta ttaacataga tatattaaaa acttttgtaa tggagaatat
      361 atgtgatatg aataataaag ttaaggttgc agtattagat acaggaatag acaaagaaca
      421 tgattactta aaagataatt tagttggagg aattgctttt gaatgtatgc atgattatat
      481 ctttatatca gataaatttg atgatgaaga tggtcatgga actgcttgtg catcaataat
      541 aaagaaagag tatgaagatg tagagttatt tgtaataaaa attttgagaa aagatggtat
      601 tacaaatata aaagttcttg aagaagcatt aagatacctt ttagacacta acataagatt
      661 aattaattta agtttatcag tcataggagt agaaagtgtt aagggcttat ttgaagtttg
      721 ttatgaattg tttagaaaag gaaaaataat agtatgttca ctcgctaatg attttgactt
      781 aagttatcca gctatgttta acaatgttat aggtgtaaga ggatttactt tagatggaga
      841 tgattcattt tggtacaaca aaaaatatga tgttcaatgt gttatggata gcaattcata
      901 tataagttgt gatataaata attcctatag attaccacca aaatgtaata gttatttatc
      961 agcaaagctt actggtaaaa tagctaagat attatcagaa aaaccaggca taacaatatc
     1021 tgatttaaat gataagcttg aatcattagc aactagaaat cattgggata gttgtgattt
     1081 tgatgagtgt agtagaattg taaactttaa attggatttg tatgacaaag aaaatgctct
     1141 ttttatgaaa gttgctgacg ttgttaggga ttgcttagat attgaagaga ataatgaaaa
     1201 actctttaag ggtagccttt ttaataagga gataggcttg gtttatgata attgttttaa
     1261 tctgttgaaa aagctagagc atagatttga cataaagttt aattatatgg acatctcaaa
     1321 atatgactta gtttcaatat atacactgac tgaattagtg gaaagatata aaaacttata
     1381 aataaagatt tgtagctaaa tttaatgtag aaggtattaa attatatgta ttctttgtcg
     1441 gtagattaaa agtaaaaaat atactataat tagatagcat aagagaaaat gccaatacag
     1501 ggggagttag ataatgaaat atgaagaagc attagagtac atatcacaaa ctaataaatt
     1561 cggtataaga ttaggattag aaaatatcgg aaagttatta gaattattag gaaatccaca
     1621 agaaactttg aatataatac atgtagctgg aactaatgga aaaggttctg tgtgttcttt
     1681 tgtttctaat atactaagag agtgtggata taaagttggt ttatatacat ctccatattt
     1741 agaaactttt actgagcgta taagagtaaa tggacaaaat ataccacagg atgatgttgc
     1801 tagaataatt gagttaataa aagaaaaaat agagattatg gtaaaagaag gctatgcata
     1861 tccaacagaa tttgaagtgg ttactgcaat ggcattttat tactattcag aacaaaaggt
     1921 agactttgtg gcattagaag ttggtttagg tggcagatat gatgctacaa atataataac
     1981 aaaatcactt gtaagtgtga tagtatctat aagtttggac catacaggta tacttggaga
     2041 tacaatagaa aaaatagcgt acgaaaaggc tggaataata aaggaaaatg gagtagtact
     2101 agtatatgac cagactgatg aagcaaagga tgttataaag tcagtttgta aagagaaaaa
     2161 agctaaatat atagaagttg attttgatga tataaacata aaaaaatcag atataaattc
     2221 tcaaatatac gattgtactg taatgaaaga aacttataga gaccttgaaa taaaattgat
     2281 aggcgaacat caaataaaca attctatctt ggcaattagt gtaataaaat atctaaaaga
     2341 cataaataaa ttagcaaata taaatgaaga atctataaga aaaggactta taaatactaa
     2401 atggccagga agaatagaaa agataaaaga aaatccaatt tttataatag atggtgcaca
     2461 taatgaagat ggagcaaaat ctttggcaaa agcacttgat aagaatttta aaggtagaaa
     2521 attaacctta ttaataggta tgttagaaga taaagatatt gatagtgttt tagagatatt
     2581 gttgccacat tttaataagg taataacaac aacaccaagt aacccaagag ccataaattc
     2641 agatatatta agagaaaaag tattaaaata tgtagatgat gtaacatcaa agcatgagat
     2701 agaggatgca gtaaattata cattagaaac atcaagtgag gatgatatta taataagtgc
     2761 aggttcatta tatatgattg gtacagtgag aactttagta aagaaattat aattttataa
     2821 aataagtata caaataaaaa tataaaaaag agttgtctaa aaaatggaca actcttttta
     2881 tataaaataa gtttttaata attttactga tgtttatata agtaattata tagattgacg
     2941 tatagcttta gataattggt ctgggcaaga agtacctttt ccaccacagt caataccatt
     3001 taatttatca gcaacttcaa gagcattttg gccaattatt aagcttctaa gtcctgataa
     3061 attaccatta catcctccta taaattcagc atcaacaatc acattatttt catcaacttc
     3121 aaaaatcatt tctctacaac atacaccact tggattaaat gttactttca ttaaaaagac
     3181 ctccattaaa tattatttct ttttttaaca ttaataataa taccaaatat atgcgtaata
     3241 atgcaactat aaagcataat acaagctaat gaaataatat attattgtag tatatgtatt
     3301 gtagagagga ggggttatat gaaaatctat atttctaact acttatcaaa gagtattagc
     3361 atagttgatt attctacact tgaactagaa aaagaaatag tattagaaga taatatatat
     3421 ccacaccatt tttgcataga aaaagaaaaa aatttaatat acatacctag ttcaagtaat
     3481 gggatactct atgtcttaga tttaagtaat gataaaataa ttgatactgt ctctatagga
     3541 ggaagtctta gtcaagttgt acttagcgat aatgaattgt ttgtggcaaa tgaagattct
     3601 aatagtatat atgtactaga taaaaacaca ttaaatccaa taggaattat tggagttgat
     3661 aatatgccac atggatttga ctttgataga gaaagcaaaa agttatatgt accgtgtata
     3721 aactcgatag tatgtataga tacaattaat aaaaatatac aaaagaaaat aaatatggac
     3781 tttagagctt ggcatataga acttgataaa caaaagaaag aaatgtatat atcaacactt
     3841 gatggaaagc ttataataat agatgaagta agcatggata taaaaaaggt attatatgaa
     3901 tttttattac cagtagaaat aagatttaat tatagtggca aaaaagttta tgttgctgat
     3961 ttgggttata ataatgtaag aattttagat tatacaacga gtagatacat tggaaatatt
     4021 gaaataaatg ggattcccca aggattagaa atatctaaag atgaaaagtt actatttgta
     4081 tcagatacac aagaaaattc agtaaaagtt tatgaaactg caaataacaa attaataaaa
     4141 gaaatcaagg taggaaaaga gcctacaact ataatttgtt tgtaggcttt aatgtatgtc
     4201 tcttataagt aaatcgataa cttcggcata catataagat gcattatttt tccagttatt
     4261 gcaaataagt tttgcttgtt tctctgatga aacatttaga tttaaatcta ttaagttaga
     4321 ctgattttct atgactctaa ggttgacaat aaattcatta tcactttgtt ttacaaaact
     4381 agactttact tgagtatctg ctagtagatt ttctttgtta gaatctatat acttgtctat
     4441 tttttcagta acactagtag gtattcgatt aaagaagtat tctagacttt ctacgcctct
     4501 ttgagtaaga gcataatatt ctctgtcaga gtctttatat gtagtcaaaa acttagattc
     4561 cataagctga gataaaagct gttgtaatga aaaataattc atcatctcag tctctaatac
     4621 tacttgagtt atttgagaat tagttaaatc cattttaatt ttttctaata tatataaaat
     4681 taaaagtttg tgataggcta attcttcaga cgagttttca aacataaata tcatcctccg
     4741 tcaaactatt tctatatata atatattaaa ctaatttgta caaaattaaa agaacaattt
     4801 gtattgtatt attaatcttt aataattcat ataaaaacaa ttttataaat
//
LOCUS       DACULQ010000225         4718 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.227, whole genome shotgun sequence.
ACCESSION   DACULQ010000225 DACULQ010000000
VERSION     DACULQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.227"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1338
                     /locus_tag="JA154_16980"
     CDS             <1..1338
                     /locus_tag="JA154_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAT7997523.1"
                     /translation="NMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLEKR
                     DLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLIAI
                     NVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVNLS
                     FDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKDKL
                     TQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSLEE
                     LKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAVAG
                     SRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILSNL
                     LDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(1516..2880)
                     /locus_tag="JA154_16985"
     CDS             complement(1516..2880)
                     /locus_tag="JA154_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7997524.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(3059..4402)
                     /locus_tag="JA154_16990"
     CDS             complement(3059..4402)
                     /locus_tag="JA154_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAT7997525.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(4475..4576)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         1754 a          654 c          680 g         1630 t
ORIGIN      
        1 aatatggaaa atttagattc taaaataaat tcctatctaa ttgaagatgg ttataaggat
       61 acaaagtacg aacgattatt gatgaactat attatttcaa ttgtagattt gaatgaatat
      121 cttgaaaaac gagatttaga taaagaaaag ataaataatt tagcacgtct tatatgcaat
      181 tacttgctaa gtgtagaatt taaatatata ggtaataaat tagaattagt gaatcgattt
      241 ttacctactg tagtaaatta tgggactaac tttgaaagtt tatattcaat tttcctaata
      301 gctataaacg tcttaaaaga tgaaaatata gagtgcaata agattgaatt tgaaaaagaa
      361 ataaatggta tcttgctcag tgaaaaatgg aaaaatatat attttgaagc attaagagat
      421 gctagtggaa aaacagaaga tattataaaa atgtcagaaa tttatgatgt aaacttatct
      481 tttgatgact tgttgccata tctaaataga gatattagag attttgaggt atattggcat
      541 atatctaaaa agggaacgac aagttctaga ttaaaattat taaatttttt tgaagagacg
      601 tttaaaatag atgatttaat tggaaaaatg aaagatatag aaaaagataa attgactcaa
      661 gaatattatg atgatatgtt attttttatt gtgcttaaag gaagtaaatc actatatcct
      721 gaaggaaaga atatatcttt aaaaggaata tttggaaata taaatgaagt aagaaaagaa
      781 tccataaata tcttaaaaag atacagagag aaactatctc ttgaagaatt aaaaattgtt
      841 aaagaagcgt atgaaaaaga aaaaaatgtt atattgaagg atgaattaag aagagtatta
      901 tatgaaagta ataatttaaa aaaagaattt gtaaatattg aaaaaataaa agtagatgaa
      961 catggtaagg atatctattt gacttcaata gctgttgctg gttcaaggtt tagaaataga
     1021 gaatatcttg aaaaagaatt ggagaaaagt aagatatact atttaactag agagaaagat
     1081 aatctatatg atgaaaaagc tattaaaata gttggtgaaa ctggatatgt gataggctat
     1141 gtcccaagaa aagaaaacta tattttgagc aatttattag atggaggaaa actgctgtat
     1201 tgtagagtaa ctgaatataa tttatatgaa gattgtatct acgcaaatgt gtacttgagt
     1261 tataaagatg taattgaaac agttgaaaac tctttaaaga tggttttaga taagagtaga
     1321 ataaaactta taaactaaga cgtttatata ttgcttagat gatgtaatcc taactgattg
     1381 gttatttttt ctggaattat ggtgtatttt atgagcaatg ctaatgaata taaaataaaa
     1441 aaacaattgt tttgtatata tgatgtatat aaatatattt atatattcat atttacaaaa
     1501 caattgaaat ttatgttatt ttataatttt aaatttagat ggaattttaa cttctgcttt
     1561 atcttttatt ttagaagctt gttttttaac tttatttaat aattcagatt catctacaac
     1621 taatactttt ctttccttca tcaaccattt accatcacaa atagtagagt ctacattttc
     1681 actagtcata gaataaacta tatttccaat tggattatgc attggtaaac aatgaattgt
     1741 atctggattt agaataatca tatcagcttt ttttcctact tcaagactac caatttcttt
     1801 ttctagtaaa gcacattttg ctccatttat agtagccatt tctaagactt cttcagctgg
     1861 tacgacagtt gggtttaaag ttcttccttt atgtattagt gatgtaagat acatgtccct
     1921 cataaggtcc attctattat ttgaaggagc tccatcagta ccaattgaaa caggtattcc
     1981 tttttctaac atttcaggaa ttgatgcaaa ccctagaacc actttcatag ctgaacctgg
     2041 gtcatgagaa acttttacat catgtaatct aaataaatca atttccttat gagtaagcca
     2101 aactgtatga gcagctaaaa gatttggtcc taatacacct agtttatcaa ggtattcaac
     2161 tgttccaact ccattgtttt gctttacaaa tgaaatttca tcagctattt cagcaatatg
     2221 catatgtata cctgtattgt atttatcagc tagttcttta gttctaacaa gtaattcttc
     2281 agttgtatta aaaatagttc ttaatgcaaa ccatatttta attctctcat tagatgtatt
     2341 attaaactta tggaataagt ctaattgagt gttgatttcc tcatcagtag ttttttgcca
     2401 tgcctcagga agaccatctc ctgtatccat aactgattta gctaaacagg ctctaagtcc
     2461 agttttatca acagcttcaa ccatagcatc tacatattga ccaccagatt ctaaaaatgt
     2521 agtgacacca gattttatca attcaacaca acatgcagta gatgaaatca aggaatcttc
     2581 ataattgaag ctactttcgt atggccaaat tctttctcta agccaagtta aaagtacaac
     2641 atcatctgca agaccacgtc ctaattgttg agataagtga acatgagtat tgactagccc
     2701 tggcattagt atttttcctt tagcatcata tatctctgca tctttatcta caatattaat
     2761 atcaaactta ccaacctctt ttataatatt attttcaata agtatgcttc cattttcata
     2821 tacctctcta tctgcattca tagtaattag atatgcattt tttataagta attgtgacat
     2881 aaacaccact ccttttctat atatttttat atctatatat tatgataata aattttctat
     2941 acaaagttaa tagcaaaatc aatttacata taaagtgatt ttgctattta taaatttaaa
     3001 ataaataaga ttttattcta tataatttaa aatattgacc tagaactatt taaaaatatt
     3061 aacttagaac taagtttaca actgcaagta aacctataat ccaaagtgta atactgattt
     3121 tatcagtttc tttagctact acatgcatta ttatatatga aataaatcca aagcataaac
     3181 caatacttat actataagtt aaaggcatca ataaaactgt aacaaaagca ggtacagcta
     3241 ttttgctatc agcaaaatca atctgtttta tgctttcaaa catgaataca ccaaccatta
     3301 caagtgcagg agctgcagcg taagtaggaa ctataccaag taatggtgta aatataagtg
     3361 tagctaagaa taatgctgct gttactattg aagcaagtcc tgttcttgca cctgctgcaa
     3421 taccagaagc agattctcca aatgttgtaa cagtactagt tcctaagaat gagcctatta
     3481 aagtagaaga aacatctgca tataacattc ttcctaaatc ttttatttca ccattctcat
     3541 cttcaagacc catttgttta caacaagtaa taagaaatgc taatgaatca aacatgtcta
     3601 taaacatgaa agagaatata ggtccaatta atgagaattt caatgcacct acaatatcta
     3661 atttaaaagc aactggtgct atactaggag gtgtcgagat tattgctgtt ggcaactcca
     3721 caaatccaaa tataacagat attattgttg caactactat acttatcaat attccacctt
     3781 taactttttt atattctagt attgccatta agataagagc aaaaagagct attaaaactg
     3841 ttggtgtaaa atctgcaagt gaaactatag tatctggatt agctattata acacccatat
     3901 tttttaaacc aacaaaagca aggaaaagac ctatacctgc tgaagatgct actgccaatt
     3961 ctctaggaat tgcgtctgca attgtctttc gaagacctgt tactgcgagt gtaaagaaga
     4021 cacatccaga tagaaaaact accccaagac ctgtttgcca tgggattcct gcacctataa
     4081 ccaaagagta tgtgaagaat gcatttaatc ccataccagg agctagagca aatggtgcat
     4141 ttcctaaaat cgcaaatata agtgtaccaa tggctgtagc tagtatggta actgttataa
     4201 gggcggcttt atccattcca gcttcagata atattgctgg atttacgaaa actatgtatg
     4261 ccattgttaa aaatgtagta actccaccta ttacctctgt tttaaggcta gagccacgtt
     4321 cgctaaattt aaacattttg tctaaaaagc ttaatgatga attatttcca cctggttgta
     4381 cattgttagt gtttggtacc atttttacct ccaaaaaact ttaagatata aacaaaaaaa
     4441 tatatattca tatagataga atagaacata tattcagagt tgacattcta ttcatagaaa
     4501 acccatttaa ggtgagtttg tagaaactat tagaccgtat tactaatatt atatgaacat
     4561 tttctatttg tttttctgtt gtattatatc gtaatattgt attatttaca ataaatataa
     4621 taaaattatt cgtttttttg tcgattttat aaaaaattaa ggttatttat tgaaaaaaat
     4681 tactttttcc aataattgaa tatgtatgaa tataatgt
//
LOCUS       DACULQ010000226         4703 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.228, whole genome shotgun sequence.
ACCESSION   DACULQ010000226 DACULQ010000000
VERSION     DACULQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.228"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            12..221
                     /locus_tag="JA154_16995"
     CDS             12..221
                     /locus_tag="JA154_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAT7997526.1"
                     /translation="MYIDKKRAIRNEWRIKEATLMSIAVIGGSIGSIIGMYSFRHKTK
                     HIKFTFGIPFILFLQFLLVYFYILK"
     gene            complement(324..743)
                     /locus_tag="JA154_17000"
     CDS             complement(324..743)
                     /locus_tag="JA154_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2992 domain-containing protein"
                     /protein_id="HAT7997527.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(1010..1792)
                     /locus_tag="JA154_17005"
     CDS             complement(1010..1792)
                     /locus_tag="JA154_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenazine biosynthesis protein"
                     /protein_id="HAT7997528.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            complement(1857..2618)
                     /locus_tag="JA154_17010"
     CDS             complement(1857..2618)
                     /locus_tag="JA154_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HAT7997529.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            complement(3666..4496)
                     /locus_tag="JA154_17015"
     CDS             complement(3666..4496)
                     /locus_tag="JA154_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7997530.1"
                     /translation="MLNKLCMYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGA
                     SRNFNFIEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSIN
                     YAQNRNKETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSL
                     KPGGKLILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTL
                     VITEKDENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK
                     "
BASE COUNT         1577 a          781 c          452 g         1893 t
ORIGIN      
        1 ctttcttttc aatgtacata gataagaaaa gagcaattag aaacgaatgg agaataaaag
       61 aagctactct tatgtctata gcagtaattg gaggtagtat aggttctata attggaatgt
      121 attcttttag acataaaact aaacacatta aatttacatt tggtatacca tttatactat
      181 ttttacaatt tttattagta tatttttata ttttaaagta attgaaatat aaaaccttat
      241 tcttttcaat aaattaaggt gtataaaatt aaaaatttta tacacctttt tctagcaatt
      301 attaaaaaga tatttaatta aaattaatga cccttatgtt tagcttttct cttttcttgt
      361 ttcaaattaa atagtctttc tttttcttct tcttttctat ttttagctag gttttttcta
      421 tcctgttttc tttcttcata ttgaagctta agagcatttt gagccttagt ccctatagtt
      481 tctttttctt gttccttttt tacttttctc tgcattcttt tgtatccaat gttgtctttg
      541 gtcacatttt tttctaattt aatgctacca aaatctaatg agaaaaatct ttcaagtata
      601 aattcataca cttctacttc tttaggttct gctccaaaaa ctaccctaca tgcttcatat
      661 tttttaccat cttgtctttc aaatatacct acccaaaatg gttcttcaaa taagactgtt
      721 aattttccac taattttgtc cattgctagt tcctccttaa attactttct aaagaacgga
      781 caaccaagga ggcaggttac tgacagctat tgctgcgtct ggactaccaa ccagactgtg
      841 tttttatctt tatagattaa atattatctt ttttaatcat gcttgtcaat cttagtatca
      901 acatctatca tattgattta agtgaaatca agttttttaa ctctacttta ttattgttaa
      961 cactaagaaa attaattatt tttcataata aattagttat tatacttatt taaatatcaa
     1021 tctttattgt tccctgcata aacaagactg cttcaccaga aattttcaca gtattatcct
     1081 tcaacacttc acactgaatt ataccaccac gattagaaag ttgagcagct accattttgt
     1141 gttttcccaa cttttcagac cagtatggaa taagattaca atgtgatgaa ccagtaacag
     1201 ggtcttctga atctaattct ggacaaaaat atcttgatac aaaatctgta ttacttcctt
     1261 gtgctgtgat tattatacct aaccagtctg tcaatttacg caattgtgcg taattaggtt
     1321 tataatttat aacttcttgt tcactgttta gcaataagat taaatctctt gaagaataaa
     1381 cagcacttgg tacacagcct atcaaattag cttgttgtgg tgataactct atttcaatag
     1441 gcattatttc tggaaatatc atttcatata gatttccatt tcgtgtaact tcaagctttc
     1501 cactttgagt aaagaaatct atccttttta cattaacatc aataaaattt gatattacat
     1561 aagctgctgc taaagttgca tgaccacata aatcaatttc tgctttagga gtaaaccatc
     1621 gtaattcata atttccttta cctttaacta caaatgctgt ttctggtaaa ttgttttctt
     1681 ctgctatttt ctgcattaac tctaaaggta ttcttctgtc aagaacacaa actccagcag
     1741 gatttccttt aaatagttta gttgcaaatg aatcaactat gtagtattcc attatttaac
     1801 ctctctttct aacataaaat atacatcaat ttacattcaa taaaacttta aatttcttac
     1861 atatattttt taatattact ctgttcaaca acttttttac ctaacaatgt aattgctttt
     1921 ttagcacaat tatttacaca acgataacac attgtacatt gcccatttgc aacaatcata
     1981 caatttttaa ttactagatt cttcattgga cataaattaa cacatttacc acatacaata
     2041 cattcctcag tattaatttt tagtttatct gtatacatat ttgttttgtt gataaaatac
     2101 aatcgttgtc caaataaccc tacaatatga ctcaaaaaac ctaacccttc ttttggtggt
     2161 gacccatgtt tcagtttttt agcagcttta gttactttct tttctgcttt tattacaagc
     2221 tttctattat actctaaaga atgttttaat accttttcat ctcctatact atctggcatc
     2281 ttcacatgta atccaccaac agttatagca ccgtattttt ttaatattct agataacaca
     2341 ccagagccat ctccactaaa tagagccata gttgcaataa taaaaacacg tttaccattc
     2401 cacaaagttg aatgatttat tataaaatct ttcaatattt ttggcacatt actatattgc
     2461 accggataac taaaaataat attctcatga ttattgatgt gctgtaaaag ttcattatct
     2521 tcaatagaat aagaatttgc tgttatatca tattcttgta aaaatttttc tacacagtat
     2581 cttgaatttc ctgttccact aaaatatatt cctatcatag taattctcct aatagctaag
     2641 attatatagc tctattctta aaattctgac tttttatatt atctaaatta agtagtttat
     2701 ttaaataata taaacttaat tcaacaagtt acctactgaa cctcccatga ctaaagtcat
     2761 agggttcctg cttgatagaa ttttgcatag tcaatttact aaatacttgt ctaattcttt
     2821 ttttatcatt ttaaatatac attttaataa tattagtaag ctatacttcc ttaagcatat
     2881 attttcccta aagtaaaact attgatgatt attccattat ataacctaat tataaattca
     2941 gtaagttcat cacatactat acccttaatt ctcaattata aagtttccta aaaaatactt
     3001 gaaaatttaa ctatgaaaaa atttcctatt gcctaatatg tataagtaag gagtactata
     3061 tagtactcct tatattgtac tcttataaca ttgatgcctt aatattattt taatcttaat
     3121 cattattgat ttgatttaaa attataaaca agttccaata ttttaattat gtcaactata
     3181 tctcttatag ttttaataat ctcatcaatt ggtttatgtg cttgttgcac tttatcaata
     3241 gtattaatta aatccattta cacatctagc acctgtatgg caatttagca ttatccttat
     3301 cttatacttt gttgtaaatt cttgattaca taattctatt atctaactta tagctgtgca
     3361 atctacatta tctgtaaata ctttgcttta tatattttcc ctgaacctct aacattaata
     3421 taacagcctt tttgctatac acttttaact acatacttaa ttttaataac attacttgtt
     3481 tataaaaata tactatagat aaatttcaca taaaaataat aattcaagta ttgattttta
     3541 tataagcaaa tttgtaatgt actaaaaata acataaaagt ttccattttt caatatgtaa
     3601 gcttttatta tgttatttta ttgttaaagg tttcaagttt ttatttattc attttaaaaa
     3661 aattattatt tttgtaaaac taatcccata gtcatactat cgtcagaata tgcttcaccc
     3721 ttaacattac taaaaaactc aacagactta aatccaatat tttcaacttc taataagaaa
     3781 ctttcttttg aaaaatagtg attccaaaca taaaatacat tttcatcttt ttcggttatt
     3841 acaagagttt gttctaatgt attataatca ttgtatttac aattatcttg tagacacata
     3901 tatttttcat tactccaaaa tcctccatct tcatttattt cccaatattt aatttcctca
     3961 aaattattgt atttatttat tgtaaataca tctaatatta attttcctcc aggttttaaa
     4021 ctatcataaa ttttttccat taataacctt ctattttcag ttgacaaagc tccataatca
     4081 caataaatta aagtagccaa atcaaattcc tcattatagt tcatatttaa ataactttgg
     4141 aacaagtaat ttatgtttaa attagtctcc ttatttctat tttgtgcata gttaatagaa
     4201 cgttttgaga aatcaattcc agttacctta taaccttttt gtgcaaattt ctctgcatat
     4261 aaacctggac cacaacctaa atcaagtaat ttaggataat tagctggatt tgcaactgta
     4321 actatccaat ttacagaatc ctcgataaag ttaaaatttc tacttgctcc ttcaaaatta
     4381 gtgtctaagt gtgcttttaa taattgcttt gaaatatact catcatccca aaaattaatt
     4441 tcactttgtt tataaagctc tggtctttct aaatacatac ataatttgtt taacaaaaca
     4501 taccactcct taaaatttat ttttaaaata aaaaaaccac agagtttact ctgtggttta
     4561 acttcaaaaa gatgagttta aataagagta aaatagaacc aaatattttt aagcacaaca
     4621 ataaaactat agctttactt tgtttgctta ttaaatttga ttttattcac tcttttccag
     4681 caatacttat atcaacgatt tta
//
LOCUS       DACULQ010000227         4664 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.229, whole genome shotgun sequence.
ACCESSION   DACULQ010000227 DACULQ010000000
VERSION     DACULQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.229"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            231..605
                     /locus_tag="JA154_17020"
     CDS             231..605
                     /locus_tag="JA154_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT7997531.1"
                     /translation="MIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTTL
                     TLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALHD
                     DENIGKDKLDFLESYFDNLKDE"
     gene            598..1746
                     /locus_tag="JA154_17025"
     CDS             598..1746
                     /locus_tag="JA154_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HAT7997532.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1763..2365
                     /locus_tag="JA154_17030"
     CDS             1763..2365
                     /locus_tag="JA154_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAT7997533.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2458..3480)
                     /locus_tag="JA154_17035"
     CDS             complement(2458..3480)
                     /locus_tag="JA154_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HAT7997534.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3809..3991)
                     /locus_tag="JA154_17040"
     CDS             complement(3809..3991)
                     /locus_tag="JA154_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997535.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4304..>4664
                     /locus_tag="JA154_17045"
     CDS             4304..>4664
                     /locus_tag="JA154_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HAT7997536.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVS"
BASE COUNT         1834 a          585 c          658 g         1587 t
ORIGIN      
        1 tttattaaat agttttaatg tgtaaataac attctacact attgtaatta aatatgtaaa
       61 aagtaaaata tttaaataga tatattttaa aatctaactt ttaatattgt aaaaaaatta
      121 gaaatttaac aaaaagtaca aaaattcaca tttttttcat aaagtgtttg actaaaaaat
      181 tcacatgttg tagaattaag gtagcaggag ttttgaggga ggtgtaaata atgataagaa
      241 aaataccaca agcagaacta aaagtaatga aattcatttg gaaagtagat gttacagtga
      301 catcaaaaga tgttattgaa gctatggaac aaaaatatgg ctggaaacaa accaccacac
      361 tgacactttt atctaggctg gtaaaaagag gattcttaga tgctcaaaaa atagacagat
      421 atacacatta cacaataaga gtaaaacaca aagattattt aagttttgag acaaaggatt
      481 tcctaagtaa tatacatgat gactctttaa gaagcttaat ttcagcatta catgatgatg
      541 aaaatatagg taaagataag ctagattttc tagaaagtta ttttgataat ttaaaggatg
      601 aataattata tgctatatgt ttttgaaaat cttataaaga caactattgt atgtagtctt
      661 ggtatttgtc tattattatt tttgaaaaga tatttattta agaagtttag taagagattc
      721 aattattata tttggttgat aatagtcttt agaatgttac tttttctatt taattataca
      781 atagtgtatg aagtaaaaga acccaaagaa aatgcattag gaaataatat tacacaaatt
      841 gatatttcta cagataataa tcttatgctg tatgtagctt atttatggtt atttgtaact
      901 atagtcattg cagtttatac atttataaag tatacaagat ttaaaaatct agttgttgat
      961 gtatcttatg atattgaaga taatgatata aactgtatct acaaaaactt gttaaaagaa
     1021 ttaaatataa aaaagaaaat tgaattaaga ggttctgatg aattgataag tccagcaggt
     1081 atgggattat ttaaatctta catattctta ccagactatc cttatagtaa agatgaacta
     1141 acttggattt tgaaacatga attaatgcat tttaaaaata aagatatttt aataaagttt
     1201 ttagttttgt ctgttagaat aatatattgg tttaatccac ttgtatatgt aatgagcaat
     1261 aaagtaaatc tagattgtga attatgttgt gatgaaagtg ttttggtaga atgttcttta
     1321 aaagataaaa aagaatatgc actagcactt ataaagtcaa ttaagtttag taaaaattat
     1381 aatagtaaga ttttgacaac cgagtttgat aagacaaatt tggaaaagag acttgagagt
     1441 attgttaaaa agaaaggcaa aagtggaata ctaattgctg ttttagtatt tatgacttct
     1501 ataacatctt ttgttgaagt tgatgctcaa gttagaattg atgagggtaa taatggaatg
     1561 aataacaata ttaatgaatt tttattgaat aagacgactg ataaaattat aaatatttat
     1621 tataaagatg caccaaatga aattaggaaa ttttatgaag ataaatgcga attagaaggt
     1681 aaaataccta gagactatga cataatagaa atggatatga aagattataa tcaattaata
     1741 aaataattat aaggaggatt ttatgttaac aaaattaaga aaaatagtag ctattatgtg
     1801 ttgcttattg cttactttta caagtgtggc atttgctgac acaagtaaag agaaaggagg
     1861 aagtaaagag actgttccag agaaaaacct agattctttt actctttctg ggtatatata
     1921 tacatatgaa aatgcgccac aagaaataag agatgaacat aaacaaaatt gtgaagagat
     1981 aaatatagac cctaagccag atgatgagat atttgttcca gaatcagcta atctaatgaa
     2041 tgaggacaat agtaaaggtg tttatgcaag ttatactgtt tcttataata taggtgctaa
     2101 aactataaca attatgagta atgagtattt gactataagt acaacaaaag ttatacgaaa
     2161 gggtaatagc ggaaaagaag taaaagctgc acaaatcatg ttaactttac ttggttataa
     2221 tgtaggtata gatagttctt ttggaagtaa gacatataat gcagttgtaa gtttccaaaa
     2281 aaaatacggt ttgtctgcag atggaataat tggtccagct acttgggata aattgggtag
     2341 actaactgac cctactcttt cataggatta aaatctaaaa atatattaaa tagtgtctca
     2401 atatgatttt tcaatttaaa tgagacacta tttaaatata aatgttataa aactatttta
     2461 caataaattc catataactc taccatctat atccttaaca ttttgacaac cagtcaaaat
     2521 catagtctca cataactcat tcctaacctt ctctataaaa aactccacac catcaagacc
     2581 accgccaaaa gaagcagtaa caaaaggtct tcccatcaaa acagcatcag cacccaatcc
     2641 aagcatctta actacatcaa caccagtcct aactccacca tcaacaagta ttgttatctt
     2701 tccctttaca gacttagcta tatctggaag tacctcacaa gtaccaggag tataatcaag
     2761 tactcttcca ccatgatttg aaacaataat ggcactagca ccagactcta cagccatcaa
     2821 agcatcatct acagtcataa ttccttttat aataaaaggt aatctagtgg attcaaccaa
     2881 ttctctaatc tcatcaattg ttttaggaga aaaaggattt tcttgaaatt gattattaat
     2941 aagtccacaa gcatcaaggt ctacaccaac tgcaaaagca cctgcttctt cagacaatct
     3001 aattttatct attatctttg aattattcca aggctttata aatacaattc catttcccct
     3061 attatccttc aatacatcta aattatccag taaaaaagta tctacattag tatcgccaac
     3121 catagcataa attccagaat tagaacaacc tctaacaaca ggctctatgt attctttttc
     3181 ggaaacttta ccacccatgt tcaatatagt acctgataca ggtgcagcga atataggaga
     3241 actcattttt ctaccaaata attctatgga tgtatctggc tttgaaacat tatgtatgac
     3301 tctcatattt atctttattt tttctagact ttttctattt tcaatgaaag acgaaccact
     3361 tcctttacca cccataccag gaacatctcc agcacaagct aatccattac aaattttaca
     3421 tactttacat gttccattaa agttctctct agctgatttt agtaagtcat tgtaatccat
     3481 acaaaatctc tcctttttat aatattaaaa ttgagtatta ctatattgaa aatatcttaa
     3541 ttgtaaatat agggatgtta ttatgatgag ctaaactaga taatacatat tagatatttt
     3601 ttattgttgt agatagttaa attatatcat attagatagt caaattttga aaaaaactaa
     3661 cttatttaag tatacatttt gaaaaaatta aaaaagctat atatagattt ttaaagaaat
     3721 ctatatatag cttttaagtt tatgtcaatc ttttttgtta atgaataaat aatatagaat
     3781 acaatataca aaaaagtttc ttgaaatgct aagataatcc ccatttacaa agcaacatca
     3841 ttatactgtg taaatttgaa tcaacaatgt tttcaccata tttcatcata aaatcctcta
     3901 attgtgtgtc aataaatgaa taatatccac ttatactatc gatgtatgca gtcattaagt
     3961 gtatacattc atcactttta tctttataca tatatatctc cccctatata caatatataa
     4021 atttagatac caaatcgtat catattaaat agttaagtgt gtaacatcta tgcagttata
     4081 tatttatatc attatattaa taactaaagt gagttttgac aatagatttt gggaaactgg
     4141 atgttcaaag tgcaaacttg gattttaaaa tcattaatta agatagcgca taggaatata
     4201 tgtatacata acttaattat aaagattata aacaattact tttattaact ttatttaaat
     4261 ggtagaataa cttggagatt aataattata aggaggatat aatatgagtt tatataagtt
     4321 tatatatgat gataaggaat atttattaaa agaggagaac tgtagtgctt taataaatga
     4381 tgaagaaaat ccagttcaag gagtaagtat ttcaaaaata attgatatac ttaatgaagc
     4441 agaagaagtt gattttgatg tagaatatta tcaagaagca tgtccattat gtttagaagg
     4501 agtaaaagaa aagaaaaaat tctttccttt tttagaatat cacttttata ttttcagtaa
     4561 agatggagaa tatgtaataa gcaatatatc tgttgattat aaaggactat cttttaataa
     4621 actatcaagg gcaaataaag ttgataatag ttatattgta agtg
//
LOCUS       DACULQ010000228         4652 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.230, whole genome shotgun sequence.
ACCESSION   DACULQ010000228 DACULQ010000000
VERSION     DACULQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.230"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            396..4529
                     /locus_tag="JA154_17050"
     CDS             396..4529
                     /locus_tag="JA154_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997537.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISPPIGST
                     NLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDADNGTF
                     GFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSNVIGA
                     WWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVTGYPP
                     PQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFSEGES
                     AIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTNVFFR
                     DLIPNGLEFIPGTVTVDGVSVPGVDPNTGFSLPDIGGGTGVEVTFDVVVESIPNPNPT
                     NNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEELIYTI
                     KVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETRVIII
                     RGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNSAVPG
                     TLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGTLNSG
                     ISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISIIKVAD
                     EDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWNGSLN
                     IGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSADIEV
                     IKTSNMDTAVPEETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVTFQPW
                     NGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDETDIS
                     PLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYSIDNG
                     VTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNNNTST
                     SEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPASILSP
                     EYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPN
                     PDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTLIDNV
                     SDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNIAEVT
                     STTPDSNLNNNISRVQVEIKQFCCNDCGCKDCCCNCCCNDCGCKNCCCNCCKNDFWC"
BASE COUNT         1783 a          689 c          799 g         1381 t
ORIGIN      
        1 tacgatattt tgtttgatta tttataatat aaaagcatac tcttaatctt caaaatttaa
       61 aaagagtaaa aaataacaaa aaaatcactg acatttcact gacaaattgt aattttgtta
      121 ttataaatac attgaaaata cttgattatt gaaaaatggc ataaaataat acattttatg
      181 ccatttacta ttatttgata gtttttaact tatgacataa gatatttttt aacacataca
      241 ataaaatata aaaaaattga tattgcaaat aaaaaaaata catattagat gttatagaaa
      301 aaaagcgcac ttataggctt aaaatgtcac ataaaagagt tttaaagaat aatatattat
      361 gaacacaaat tttgtaatca caaaggagtg ataatatggc aacagtatca ggacaaatag
      421 tttttgatag aaatagaagt gctaccattg attctggaga ctcaggaata gccaatatac
      481 cagttgtatt acaagatata gcaactggag ttagacttgt ggttctcaca gatgcatcag
      541 gaaattatgt ttttactaat gttccaaatg gtaactatag aattgtagaa gcttttggaa
      601 ctacaggagg agtaccaaca ccagggaatt ttgcaacagc gatagtaggt ccagttccaa
      661 ttgcagaaat gccacctatt agtcctccta ttggttcaac aaacttagat ggtacaacac
      721 ctaacacact attaataaca gtagcaggtt cagatataac aaatcaaaat atattaaatg
      781 ggcctgtgat ttatacacct atagagaata tattggattc ttgcgtatct gtatctaata
      841 taaaccttat tacagatgca gataacggaa cattcggttt ttttccacca ggtacaccaa
      901 gtaatacagg acctgaaata gaaccttacc caaatgtgac gccagatttt acgtatgttg
      961 taccagaccc aactaagttt acacctttag atggagaata tacagtacaa aatattatga
     1021 caaatgcaat gagtaatgtt ataggagctt ggtggcgtat tgctgaccat actacaggaa
     1081 atgaaacagg aagaatgatg gtggtaaatg gttttaatcc tggagctgta ttttttaaat
     1141 caacagtaca agttacacct aacactaatt atttatttag cacttggatt ttaaaccttt
     1201 ttaaggtaac aggatatcca ccaccacagc taggtgtaag aatatttgac caaaataata
     1261 atatactata tcaagcaaca ttaggagctc aaattccagt taatacaaac gcaccagaat
     1321 ggaaagaagt aggtactgtg attaattctc aaaataataa tcaacttaca gtagaatttt
     1381 ttagtgaagg agaaagcgcc attggaaatg attatgctat agatgatgtt tctttaagag
     1441 aaatagctgt tccaactttt actccaataa aaacaagtaa cacagaaact gctaatgtag
     1501 gtgatataat cacatataca gttacattag ataatacttg tgatagccct ttaactaatg
     1561 tattttttag ggatttaatt ccaaatggtt tagagtttat acctggaact gtaacggttg
     1621 atggagtatc tgtaccagga gtagacccta atacaggttt ctcacttcca gatattggag
     1681 gaggaacagg ggttgaagta acttttgatg ttgtggttga aagtattcct aacccaaatc
     1741 ctactaataa tatagctaat atagattact cctatacacc tgtagaaggc ggtataccaa
     1801 atgatttttc agtagattct aatccagttc ctgttgaagt aatatcagca gatattgaag
     1861 ttacaaaatt atctgaacct actatagtga atccaggaga ggaattaata tacactataa
     1921 aagtagtaaa taatggacca ttcccatctg aaaatgtggt tttaacagat gatgtaccag
     1981 cttctattat aaatccagag tattcattag atggaggagt aacttttcaa ccatggactg
     2041 gaagtttaaa tatagggaca ttagaagtag gagagacaag agttattata attaggggta
     2101 ttgtaaatcc atcaacagta ggaattataa caaatacagc tgtagtatct tcaactacag
     2161 cagacccaaa ccttaataat aatacatcta caatagagac agaagtgaat ttattagcag
     2221 atattttagt aatgaaaaca gcagaaccta attcagctgt tccaggtact ttattgagat
     2281 atactattca agtagaaaac ttaggaccag caaatgctga aaatgtaata ttaaatgatg
     2341 atattccagc ttctattata aatccagaat attcattgga tggaggagca agttttcaac
     2401 catggaatgg aagtcttaat ataggaactt taaactctgg aatttcatta acagttttaa
     2461 ttcaaggaac agttagttta aattcttcag aatatatagt aaatacagca acagtttctt
     2521 caactacacc tgacccagat ttaagcaaca atatatctac cattattaca ccagttaacc
     2581 cacaagcagg tatttctatt ataaaagtag ctgacgagga tgtagctgtt ccaggagaag
     2641 aatttgtata tacaattgaa atttttaatg aaggaccatc taatgctacg aatgtagttt
     2701 taacagatga tataccagat gttattttaa atccagaata ttcattggat ggaggagcaa
     2761 ctttccaacc atggaatgga agtcttaata taggtacagt tgcaccaggg caattaataa
     2821 gaattatcat aaagggatta gtaagttcaa ctgctacagg agatattact aatacagcag
     2881 aagtaagtgc agatgtacca gaaccagtta cggattcatc aacagtgaca actcctatag
     2941 ttccatcagc agatatagaa gtaataaaaa ctagcaatat ggatacagca gttccagaag
     3001 aaactttcag ttatactata acagttacaa atttaggacc atctgctgca caaagtatcg
     3061 ttttgacaga tgatatacca gatgttattt taaatccaga gtattcattg gatggaggag
     3121 taactttcca accatggaat ggaaatctta gtataggcac tttggatgct ggagaaataa
     3181 gaagtattat aataagagga acagtgagcc agacagcagt aggtacaatt ataaatacag
     3241 caacaataag ttcacctaca cctgacccaa atccagataa taatacttca acagatgaaa
     3301 cagatataag tccattagca gatatttctg tgataaaagg caacgaacca gttgctatac
     3361 caggaggaag atttatttat ggaattgaaa ttgcaaatgc aggaccatct tttgcagaga
     3421 acgtaacttt aacagataat ataccagctt ctattttaaa tccagagtac tcaatagata
     3481 atggagtgac tttccaacca tggaatggaa gccttaatat aggtacttta gatgctggag
     3541 agataagaaa tatcataata aggggaacag taagtcaaac agcaataggt acaattataa
     3601 atacagcaac agtaagttca actacacctg acccaaatct aaataacaat acttcaacat
     3661 cagaagcaga ggtaagttca tcagcagata tttctgtagt aaaacgaagc aatcagactg
     3721 ttgttgtacc aggagatgta ctagactata caattgaggt gagaaatgca ggaccgtcta
     3781 ctgctcagaa cgtaacttta acagataata taccagcttc tattttaagt ccagaatatt
     3841 caatagataa tggagtgact tttcaaccat ggaatggaag tcttagtata ggcactttag
     3901 atgctggaga gataagaaat atcataataa ggggaatagt aagtcaaaca gcaataggta
     3961 caattataaa tacagcaaca gtaagttcaa ctacacctga cccaaatcca gataataata
     4021 cttcaacatc agaaatagat ataagttcat cagcagatat ttctgtaata aaattagcaa
     4081 ataaaacaga agcatgtgta ggagagcaaa tagattttgt aatagtagta tcaaatgcag
     4141 gaccagaaaa tgctcaaaat gttactttaa tagacaatgt ttcagataag cttaagaaag
     4201 cagtattttc acttgataga ggtgtaagtt tccaaccatg gactggaagt cttaatatag
     4261 gtacactacc agctggaact ttaagagtga ttatattaag aggaattata aaatcaacat
     4321 gtcttgatag attaacaaat attgcagaag ttacttcaac aacaccagat tcaaacctta
     4381 acaataatat atcaagagtg caagtagaaa taaaacaatt ttgttgtaat gattgtggtt
     4441 gtaaagattg ttgctgtaat tgttgttgca atgattgtgg ttgtaaaaat tgttgctgta
     4501 attgttgtaa aaatgacttt tggtgttgat tttataaaat aattttgtat accttaaatt
     4561 aaactgaaat aaaaaataaa ttcacatatg agtaaaatat tttataaata ttaataaatt
     4621 ttctatcttt gatatggacc acattcgtaa ta
//
LOCUS       DACULQ010000229         4648 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.231, whole genome shotgun sequence.
ACCESSION   DACULQ010000229 DACULQ010000000
VERSION     DACULQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.231"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            68..363
                     /locus_tag="JA154_17055"
                     /pseudo
     CDS             68..363
                     /locus_tag="JA154_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            771..1460
                     /locus_tag="JA154_17060"
     CDS             771..1460
                     /locus_tag="JA154_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7997538.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIRYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            1460..2377
                     /locus_tag="JA154_17065"
     CDS             1460..2377
                     /locus_tag="JA154_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7997539.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLVEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRAIVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            2450..3376
                     /locus_tag="JA154_17070"
     CDS             2450..3376
                     /locus_tag="JA154_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997540.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVNPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            3373..4053
                     /locus_tag="JA154_17075"
     CDS             3373..4053
                     /locus_tag="JA154_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter permease"
                     /protein_id="HAT7997541.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4151..4486
                     /locus_tag="JA154_17080"
                     /pseudo
     CDS             4151..4486
                     /locus_tag="JA154_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
BASE COUNT         1759 a          481 c          761 g         1647 t
ORIGIN      
        1 taatataatt ataaaaataa taaatatttt tatagtagta gttaattatt aataaggagg
       61 ggtcaaaatg cctagcatga ttatggaagt tgtaggagta ctatttatag ctattggatt
      121 aatcttgaca ttcattgaaa taaaaggaaa tttcccttct ttttatggtt ttggtcaaaa
      181 ttaaaatgct gtaggtatta tagcagcagg aattgcttta attttcttta gaaatggcat
      241 agatacatag attctatagc tacttatggt gtatttttag caatagaact tgttgtaata
      301 gttgcatatt tcttaataag aaaaaaattt actaaagaag ataaacattt aagaactaat
      361 taactaaatt atctactata aaattggaaa attaaaaaca aataaaagtt tgtttttaat
      421 tatttaaaat ccaaatttga ttatttttaa agaaactatg ttctgtataa aactagaatg
      481 tggtttcttt tttatgtata aaatattttt tagcaaaagt taaaaggtgt tttataatat
      541 aaaatagtta attgtagatg ttaataaaag aaaattatgt atattttaga taaagataag
      601 agagtatact atattttaat tcttttttca ataaagctta ttgaaaagaa ctttaactta
      661 gaagagtatc accaaattgc agatacaata tcataagcag ttatttattg acaacccttt
      721 ttaataaata tatcatttga agtatactga cgaaaaggag ttgaataata atggacatct
      781 tattggttga agatgataag aatattcgag aaatggtata cgattttctg attagtgaga
      841 attactctgt taaaactgcc attgatggaa atacagctat tgaaatattt aaaaattatt
      901 catttgatat tattttactg gacttgatgt taccaaagtc tagtggtttt gaagtattaa
      961 aatatatacg aagtgtgagt gttgttcctg ttattattac tactgcgaaa gatagtgaca
     1021 cagataaaat gctagggctt aatctaggtg cagatgatta tattacaaag cctttttcaa
     1081 tcgtagaatt gcttgcaaga attaaagcta atattagacg tgcaacagtg tatagcagtt
     1141 caagaaactc tcaagatatg gttattcggt ataaggattt gattataaac atatcaaaat
     1201 atgtagtttc aaagaatgga attgacttaa acttaacaca tacagaattt gaaattgtta
     1261 aattactagc gactaatcaa ggacgtgctt tttcaaagga acaattatac aacgctattt
     1321 ggaaagaacc ttattatgga aatgaaaatg tgctaaatac tcatattaac aggttaagaa
     1381 ataaattgtc tgatggtata gaaaaaaata attatattaa aactctttgg ggaattggct
     1441 acaaaatgga ggaagaataa tggagttatt tttagttgta atttcgattg tactatttgc
     1501 tctctactta aaagaacgaa ataaatcaaa gaaaatggaa agtgattata agtatatcaa
     1561 ttcaagactt cgtgacataa caaatgatga tgaaattaat tatattttag taccatctga
     1621 tatttcgatt gtaaaagaaa cagcagtaga tataaataaa cttgttgaaa agttttacag
     1681 caggcaaatt aattataata gaagtgaaaa agcgatattg caaatattta caaacatatc
     1741 acacgattta cgaactccca tcacagtatt aaagggttat attgaaatgc tatatcttca
     1801 aagtcaaaaa gaagaattat cttcttcaat gagagcaatt gtggaaaaaa tgcaaacaaa
     1861 ttcaaatgag cttgttaatt caataaataa cctgtttaat atggcaaaaa tccaatctgg
     1921 agatatgata ttaaatcttc aaaaatccaa tctaacacaa ttatgtcatg agattatttt
     1981 agagttttat gatttgctag ataaagaaaa ctttaatgtt gaagttaata ttgaagatag
     2041 acctatatat gcaaatgttg acacagaagc attacaacgt atactcaaaa atttaattga
     2101 taatgctata aaatatggtt cagatggtag atttttaggg atttcactat atgaaaagga
     2161 aaaacatgtt tttatagata ttgaggataa aggttcaggt atttctgaaa ctgaaaaaga
     2221 gcatattttc acaagagctt atactgctga cagaaaaaaa ggcaatggtt taggtttggc
     2281 tatttctcaa gggcttgcaa actctatggg aggttcaatt tatgtaaata gtagacctaa
     2341 tgtaaaaaca gtttttacgg taaaattgaa aagttagaaa aaagttagat ttaaaagaga
     2401 gaaaagacaa ttttattttg tatactgttt ttagaaagga gggagtatga tgaactatat
     2461 catagagacc catcaattaa agaaaatata taaagataaa gctgttgtta atgctgttaa
     2521 tattcatgtg aaaaagggag aaatttatgg atttgttgga ccaaacgggg caggtaaaag
     2581 tacagtaatg aaaatgttgc taaatcttgt taatccaaat tctggagaaa tagttatgtt
     2641 cggtaagaaa gtggcagaaa ctgattttga aatattaaaa aagattggta ctattattga
     2701 aaatccgtat ttatatgaga atttgactgc taaacagaat cttgatttac actgtgaata
     2761 tatgggttat tataacaaag aacatatatc agaggtattg gaatgtgtcg gattgtcaaa
     2821 gcaatcaaac aagaaagttt caaaatattc tttagggatg aaacaacgtc ttgcaatagc
     2881 tagagcaatt cttactaaac cagaattatt gattcttgat gaacctataa atgcactcga
     2941 cccagagggg ataagagaaa tgcgagaatt attccgtaag ttaaatacag attatggaat
     3001 aactatattt atttctagtc atattctttc agaggtagaa caaattgcgg acactattgg
     3061 aattattcaa gatggtaaac ttattaaaga aatttctatg tcagatatac acaaatatca
     3121 aacagattat gttgaagtag atgtagataa tgtggagctt gctggatact tgttagaaaa
     3181 agaatttgga ataaaagact ttaaaattac ttcggaaagt tgtattgaaa tatatgattt
     3241 acgaaaagat gtaaaagaaa tatccaaaat atttattcaa aatcaaattg gtataaatag
     3301 cattggacgt aaacaaagtt ctttagaaga ttacttcttc caaactacag gtacaggagg
     3361 taaagaaaca ctatgataca tttaatgaaa ctagagctaa aaaaagttgg gctaaaaaga
     3421 tattttttat tttcaatgct ggggattttc ttatgtatgt tttttgtctt tactggattg
     3481 aatgataaat ctacttcaat atatgattat gatgttacat ttaggacaat cagtttattt
     3541 ttctgttttt actatattat attatttgct gtgttgaatg tggcatatat aataaatgaa
     3601 tatacaaata aaacaatctt gattttattc tcttatccac ttgaccgtaa aaaacttatt
     3661 ttatcaaagt tacttttgat tacgactctt atgatatcat caatgatagt tggatatatc
     3721 tgttgtttta cattcgttgt tgtgttggat aagtatttta atttagttat tggagatttt
     3781 catatttcaa ttttatctta ttggatacca ggagcaatta aaagtattat tgtattttgc
     3841 tcattaggta tatggacatt cgttgttgga atgataagaa aatcaatacc tgttaccctt
     3901 gtcagttcaa ttatttttat caatttaaga caatttattt tagctggtac aaatacaact
     3961 caagacagtt tgatttctgt ttttattata gttggtataa ctgtgattgg tatatattat
     4021 atactaaatc ataaagttac tgaaattgac tagatactgt ggataggagg gtagttatta
     4081 attgtatctt cttgggataa taatgatttg ctattatatc agctattatt aatattatta
     4141 gagttttaat aatattgcac aaccatttga agttgagcca aagcttgatg aattaattga
     4201 atggtactat agtcagagtg aagtatctat taaagttata gctgaatttc attatagatt
     4261 tgaattgata catccatttc aagatggtaa tggtcgcata ggtagatttg ttatgctaaa
     4321 acagatgtta gaaaataatc ttcctataaa aattgtatct tgggatagtg aggaattgta
     4381 tagaaactcc ttaaattctt gtagtttagg taattatgtc cctttaattg aatatttatc
     4441 aagtttagaa gattttagag aagtatataa aatgttatgg aaataattgt ttgcagcaaa
     4501 aaaagttttt atataaatat atttttatct atgctattta acgctaattt cttagttatt
     4561 cttagcttgg actataccca ctattttaac tatataaaat agtggatata gtttaactat
     4621 ctctttataa ctatttttat gtatctta
//
LOCUS       DACULQ010000230         4549 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.232, whole genome shotgun sequence.
ACCESSION   DACULQ010000230 DACULQ010000000
VERSION     DACULQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.232"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            302..1603
                     /locus_tag="JA154_17085"
     CDS             302..1603
                     /locus_tag="JA154_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase DnaB"
                     /protein_id="HAT7997542.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1679..1954
                     /locus_tag="JA154_17090"
     CDS             1679..1954
                     /locus_tag="JA154_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997543.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3397..3627
                     /locus_tag="JA154_17095"
     CDS             3397..3627
                     /locus_tag="JA154_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAT7997544.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3620..4201
                     /locus_tag="JA154_17100"
     CDS             3620..4201
                     /locus_tag="JA154_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAT7997545.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSI"
BASE COUNT         1778 a          367 c          892 g         1512 t
ORIGIN      
        1 cgatatatta ataattggca gtaagttata tcaaataaga tattactatc aagtagtgaa
       61 ttatattgaa caatatatta ataattggta gtaaagtata tcaaataaga tattgctatt
      121 atgtagtaaa ttatataaaa caatgtatca gaataaatag tatattaata atttgtagta
      181 tatcatgacg aataatacag tcataacaaa tagtgagtca taacaaataa tgtatttata
      241 attgataatg tatttaaaaa tcaaaatata aaaacttata aggaaagaga gattaattta
      301 tatgggtaaa tcaaaagatg ttgaaataga gaagtctatt ttaggcacaa ttcttttaga
      361 caataaatta agctataagc tagatgaatt aaatgaaaac atgtttatga atgatatatg
      421 tcttgagata tttaaaatta tgaaagaact taaaaaagaa aacatagtga ttgatgtggc
      481 cactgtgaaa tcaaagatag atagaaaatc tctagcaata aaaacgagtg atgtgactaa
      541 tctaattact tggggtcaga attttggtct tgatggtcat attaaaattt taaaagaaaa
      601 tttagctaga agaagcataa atcaaaattg ccaaaacctt ttacacagcc taaaccttgg
      661 agaaaatatc gatacttgta tttacaaatt tgaaagtaat ataaaggaga ttttggacaa
      721 ggatacttat gaaaatgatg atgtaaatag tatagcaggg aaggtgcttg attttttaga
      781 aaacaaaaaa gatattggat ttaaatttgg tataaaatta cttgatacca ctataggagg
      841 tctttttaaa ggagagctaa ccacaatagc agctaaatcg ggggttggga agactgcttt
      901 ggcacttcaa ataatgttaa attcatttaa gcaaggtaaa aaaactttat ttataagtag
      961 agaaatgacc agtgagcaag tatttatgag aaatatatgc agagtaactg gtgtaagtac
     1021 aaggaatatg aaatctaaag aaatagatga aaatgattgg aaacttattg taaatgctat
     1081 aggtgattta agcgaaaata acttaattta tattaatgat aagatagata caattagtgc
     1141 aattagaaag aggataaggc aggtaaagcc agatttatta atagtggatt atgtgcaact
     1201 ccttacatct cagaaaagca tggataagag agaaagggag gtagctactt tttcgagaga
     1261 acttaaaaat atgactttag attttaatat acctgtaatc caattaagtc agttaaatga
     1321 tgaaatgaaa gattcaagac cttatggaga tagacctatg agggatagca aagccatcta
     1381 ccacgatagc aacaatgtgg tttatataca tcaattaaag gggagtgatt atgaagaagc
     1441 agtgagagat attggagaaa gtgaggaggc agttagagca tcagaataca ggggaattaa
     1501 gatggttgat ttaatagttg cgaagtgtag agatggacag actaggcata agcatttttg
     1561 ttactttggg gataagttac attttcaaga actaaattat taggtcaata aagtagtaat
     1621 aacaagctta ttaaaagtcg ggaaatagca aatagattat aaaatatgag gtgaaaatat
     1681 gttggaatta agaaaaatta tagaattact tgaagaacac aagatagaag taagtattcc
     1741 aaaggagtta cataagtata gtttattgga ataccttgaa ttgtgtcaac aaagtggggg
     1801 aacatatttt tatattaaaa cactggaagg ggctttaaaa gaggctagaa atgagcttat
     1861 gattaaattg tttaatggga aaaattatat ggaattagcg aggatgtttg aaatgagtgt
     1921 tattaatgtc aggcatattg ttagaaaaaa atagatattt ttggagaata caagtgtgat
     1981 atacttctga aatagaagtt attatagttg aaataaaatt gtggttaata aattaaagtt
     2041 gtgtataagt ttaagattat agaggagtat aagtgtgatt aacagcatga ttttgtgaat
     2101 aaaattgagg atatatatga gttaaatgtg tgattaataa ggtaaggttg tgtataagat
     2161 tatgagtatt taaaaggagg ggctataatt aatagtgtag agttgtgtgt ttgcttaaga
     2221 ttatataata atttaataga ttaaactata ctaataaggt aaaatcgtgt gtttagttaa
     2281 agcttattat aaaatcttga tatgtgatta atgatgcaat atgtgaataa agttaagatt
     2341 ataatagatt taacctgtgg ctaatagaat aaaattgtga ataaagttaa gattataata
     2401 ggtttaacct gtgattaata gagtaagagt gtaaatagag ataaacttca atatgagctt
     2461 aacctatgat taatagagta agagtgtaaa tagagataaa cttcaatatg agcttaacct
     2521 atgattaata gagtaagagt gtaaatagag ataaacttca atatgggttt aacttgtgat
     2581 taatagagta gggtgtaact aaagataaac ttcaatatga gtttaacttg tgattaatag
     2641 agtaaggtgc aactaaagat aaacttcaat atgagcttaa cctatgatta atagagtaag
     2701 aatgtaaata gagataaact tcaatatggg cttaacttgt ggttaataga gtaagagtgt
     2761 aactaaagat aaacttcaat atgggtttaa cttgtggtta atagagtaag aatgtaaata
     2821 gagataaact tcaatatgag cttaacttgt gattaataga gtaagagtgt aaatagagat
     2881 aaacttcaat atgggattaa cttgtgatta atagagtaag agtgtaacta aagataaact
     2941 tcaatatgag cttaacctat gattaataga gtaagaatgt aaatagagat aaacttcaat
     3001 atgggtttaa cctgtggtta atagagtagg gtgtaactaa agataaactt caatatgagt
     3061 ttaacttgtg gctaatagaa taagattgtg aataaagtta tagttttaga tgggtattaa
     3121 cctgttacta atagaatatg attaggtgta aaattataga gctgtataag aaaatattat
     3181 agatatatta aatattagat aataaatatt tatatagata cacaaaagaa aaggcgatat
     3241 gaagctaaac ttaatgactt catatcgttt tttatttgaa actcctttgt atttagagtg
     3301 tgatttttaa tattgaggtt ttatgtgatt aagtcacaga taaaaagtaa gtatcttgat
     3361 ataattctta tagattaata atatagggag gttattatgt ctaataaaaa aaagaagtat
     3421 gcaaatattt tagcaaatga gtgcgttgct tgtggtagct gtataaaagc atgtccaaga
     3481 agtgctatct cagttccatg tggcattagt gcaaaaatta atagagattt atgtgttgga
     3541 tgtgggattt gtgaaaagat atgtccagca tctgtaattg agattataac aatattaaaa
     3601 gaggaagaag gtaagtgtca tgaataataa aaaacattgg tatgactatt tatggatttt
     3661 ttcttcaata tatttgatat tgggatttgt aaatattatt ttcgcttgga ttgggctaat
     3721 ttgtttcttt gttccactag ctatttctat tgttaaggga aataaagctt actgtaataa
     3781 gtattgtgga agagggcaac tgtttaattt gcttggaaat aaattgaagc tgtcaagaaa
     3841 tagagatatt cctaaattta ttagaagtaa gtggtttaga tatgggtttt tgacattttt
     3901 tatggtaatg tttgccaata tgcttttttc aacttattta gtgtttgaag gaagtagaaa
     3961 ttttaaagaa gtagtgactt tgttgtggac tataaaaatg ccttggcaat gggcgtatca
     4021 tggaacttta gtttcacctt gggtggtaca gtttgcattt ggtttttata gtgttatgct
     4081 tacatcaact gttcttggtt tgattactat gatattgttt aaacctcgtt catggtgtgt
     4141 ttattgccct atgggtacta tgactcagat gatatgtaag gcaaagagta agtctatata
     4201 gaaaggcttt atgtgatatt tacagttttg ttagagatga gtagtaggtt tgaatatata
     4261 tgacaaatat atgagtatag gggcatagat ttagtttgtg taaggtaaac tgatttatgc
     4321 ttctttttta tgtcttagat tattggctaa gtggaaacaa tgaatttaga tattaagaat
     4381 tattcagtta tttgaagaaa atagaataat aattaacatt atatgaatgg atattcatta
     4441 catataagcc agttaatagc aagatttatt taatatgaaa taggtcaagt atttaaacta
     4501 aataaggtca tgacaattaa tataagtata gatataaaaa gattagaag
//
LOCUS       DACULQ010000231         4490 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.233, whole genome shotgun sequence.
ACCESSION   DACULQ010000231 DACULQ010000000
VERSION     DACULQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.233"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..891
                     /locus_tag="JA154_17105"
     CDS             <1..891
                     /locus_tag="JA154_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997546.1"
                     /translation="NPQSKLPRVEEISYREKIIIIHKIYSFMENYIKFPKENLPNRKV
                     MSDININDIESLAYKTREFWDLNSTPIVNMVSLLESKGIIISGMNVDRKGATIFTQKQ
                     RISKESKYLISLGNDKKSASIRNYTLACELGYIISSELRIPSKQFSEDEYACAFLLPK
                     ESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYNLEKINYKKYNYLMNEMDKKGWL
                     IEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVSSLEEFGINLYPEDLELLMGLKK
                     GLLSQEVNKKSKVIKFDGKK"
     gene            complement(1008..1976)
                     /locus_tag="JA154_17110"
     CDS             complement(1008..1976)
                     /locus_tag="JA154_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAT7997547.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            2311..2499
                     /locus_tag="JA154_17115"
     CDS             2311..2499
                     /locus_tag="JA154_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997548.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            2759..3958
                     /locus_tag="JA154_17120"
     CDS             2759..3958
                     /locus_tag="JA154_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HAT7997549.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIESDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            4010..>4490
                     /locus_tag="JA154_17125"
     CDS             4010..>4490
                     /locus_tag="JA154_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAT7997550.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     "
BASE COUNT         1810 a          443 c          767 g         1470 t
ORIGIN      
        1 aaccctcaat ctaaactacc tagagtagaa gagatttctt atagagaaaa aataattata
       61 atacataaaa tatattcatt tatggagaat tatattaagt ttccaaaaga aaacttgcca
      121 aatagaaaag taatgtcaga tattaatata aatgatatag aatctcttgc atataagact
      181 agagagtttt gggatttaaa tagtactcca atagtaaata tggtaagttt gttagagtct
      241 aaagggatta taatatcagg tatgaatgta gatagaaaag gggcaactat ctttactcaa
      301 aaacagagaa ttagtaaaga atctaaatat ctaatatctc tgggtaatga caaaaagtca
      361 gcatcaatta gaaattatac tttggcatgt gagcttggat atataatatc aagtgaatta
      421 agaataccaa gtaagcaatt ttcagaagat gagtatgcct gtgcattttt attgccaaaa
      481 gagagttttt taaaagattt gactcatcca gaagatttag attattatgt agaactaaag
      541 aaaaaatgga ttgtacctat atctgctatg atttttagag catataatct agaaaagata
      601 aattacaaaa aatacaatta tcttatgaat gaaatggata aaaaaggctg gttgatagaa
      661 gaaccactag acaaaatgaa aggttcaagc ccaacgtatt taaaaagagc agttgaatta
      721 ttgatagaaa ataaaataat gagtgtaaat agtatagtat caagtttaga ggaatttgga
      781 ataaatcttt atccagaaga cttagaatta ttgatgggat tgaaaaaagg attgttatct
      841 caagaggtaa ataaaaaaag taaagttata aaatttgatg gtaaaaaata ggtttatatt
      901 ttagtattaa aattaaaagc aggtacaaat gatttaaaca ttaacttagt taaaagtctt
      961 ttcaataagt catttacctg cttttagtgt aagaaagaat attgaatcta aagtgttttt
     1021 gaaagtgcaa tatattccct taaaacagaa caagatttat taaattcgtt ttgctcatat
     1081 tcagtcatat tgatttcaat aatttcttca ataccatctt ttcctataac acatggaaca
     1141 cctgcaaata catttctttc gttatattga ccttctagta atgtagaaac tggaagaact
     1201 tttttctcat tgtgtagtat agcttttaca atttctacta aagcagtacc tataccaaat
     1261 tctgtacaat ctttaccatt tattatactt attcctgttt ttttattttt ttcaactatt
     1321 gatggtaagt ctaaattgct ataagtactt gggttgtctt ttatcatgtc taagattggt
     1381 ttaccaccaa ttgaaacatg agaccaagga accatttgag aatctccatg ttcacccata
     1441 gaataagctt gaattgattg ttgagcgatt ccagtttctt cagataaaat ccttctaagt
     1501 cttgttgaat ctaaagctgt tccagttcca atgactttgt ttttaggaaa gccagatttt
     1561 tcccaaatat aatttgtaac tacatcaaca ggattagata tgactacaaa tataccatca
     1621 aagccagaat caactatggg ttttactata gattttatca ttccaatagt tggtcttagt
     1681 aaatctaatc tagttgtgtt ttgtctacgt aaaggccctt ctgaagagtc tgcaacactt
     1741 atcactacta tgtcagaatc tttacagtca gaaaaagttc ctttttctat atgtacttta
     1801 tgtggcagat atgaaactgc atcagctaaa tctaaagctt gagcttttga tttagattca
     1861 tctatatcaa tcataaagag ttcatcacaa actccttgag ttattaaact aaatccacaa
     1921 tgagaaccaa catgccctga acctacaata gatatttttc ttggctttat tgccatttta
     1981 aatcactccc cctaaaataa cgatacattg aaaattatag atgttattaa aagctgtttt
     2041 tggatatttt aatattctat tatttcagaa aatttattta atatgatttt atactaaaat
     2101 ttttaaaaaa gcaatgttaa tatactctct attagtgaat attaaagtat tatatgtata
     2161 ttaataatta ttaaaatatg taatgtcact gatagttaga atatttaagt gctaaaagat
     2221 gtgtataaat aataatgatg tcagtttata atgatattaa attgagatat actgtttctc
     2281 taaattaaaa aataaaggag taaatgtgtt atgaattatc atacaaaaga agaattggtg
     2341 gaagcactga gagtagtatc ttcaagtatt attaattgtg gaaaaggaca gaagaaattt
     2401 tctgaggaga catctcatca tacacgtttt aagaatatta tagaagtaat gtatatttca
     2461 aaatcattaa taatggatga aattagtaaa agagattgaa gcttattttg caatttaaca
     2521 tgttttaata tacattttat tatataagaa aataagtaaa catacatgtt ataaaatatt
     2581 ggaataatat acaattataa atttgttaac actaatatct atatgttaaa atatagtaga
     2641 agatgttagt aaataatagg atgagttcta ttttaataca attaaattaa gcttagtttg
     2701 aaatttgtat ctaaaaagta aagtaatcta gaattaagaa aatggagata gaattataat
     2761 ggaaaagtta tattatacaa atcagtatat aagagatttt acagcagaaa taattgaaat
     2821 aaaagaagta gaaaataagt atcatgtttt attagataag acagcttttt ttcctggtgg
     2881 aggaggtcag tttggagacc tgggaaaaat agagagtgat gatgtaataa atgtgtatga
     2941 agatggaaaa aaaatatacc atgtattaga taaaaaacct ataaagatac ataaggtaaa
     3001 gtgtagtatt gattgggata gaagagaaga tggaatgcat caacattttg gacaacatgt
     3061 attgtctgga tgttttttta aaacttttaa tgcaaatacg acaggttttc atttgggaaa
     3121 tgagtatagt acagtagata tagaaggtaa tttagaagag gataaaatta gagagataga
     3181 gttgtttgcc aatgaaatta ttcgtcaaaa tatacaagta gagacattac ttccaacgaa
     3241 aaaagaacta aaaaagattt ggttaagacg tgatttgcca aatactgatg aagagataag
     3301 agtaataaaa ataggtgatt tagatacgaa tgcatgttgt ggagtgcatc caaaatcaac
     3361 tggtgaattg agaatgataa aaataaagaa atgggaaaaa cataagtcaa gtacaagaat
     3421 agagtttcta gcaggtaaaa gggcagtgga tgaagtattg aagagggata tatatttgac
     3481 taaaatatgt agatatttaa gctgtagtga ggaagatgct ataaatggta ttgtaaatct
     3541 aaatagtaaa gtggaagagg ctttaattaa aaaaagaagg cttgaagaag tagtatctac
     3601 atatcaggtc aaagaaatga ttgaaaaagc aaataaggtt ggcaatattt cggtcattaa
     3661 aaaaatttat gatgatgaag atttgaaata tgtaaataag atagcaaata aaattgcaga
     3721 atatgaaaat agtattgcat tattagctgt taaatttaat gataaaataa atttagtttt
     3781 tgcatgttct aaaaatttaa aagatattaa tatgggagtt ttattaaagg atgcaataag
     3841 tcttatagat ggcaagggtg gtggaagtaa agtattagct caaggaggag gtaagaataa
     3901 tggaaattta gaggctacac ttgattatgc atttatgaag atagaaaaaa ctctataaat
     3961 atattatata aaaaacctta gtagataaaa tagatatgga ggaataagta tggagcaaca
     4021 aaaggactac atagagttat atgaaaatga agaactaata gagataagag aagcacgtat
     4081 ggatgatttg gatacaatag caaaatttaa ttataatttg gctaaagaaa ctgaaggaaa
     4141 agaacttgat atggatgttt taactaaagg tgtaaaagca ttgttattag atgagagaaa
     4201 aggaaaatat catgtatata cagtatttga taaagttgtt gcacagatta tgtatacata
     4261 tgagtggagt gattggagaa atggaaattt cctttggatt cagagtgtat atgtagataa
     4321 ggaatataga agaaaaggta tctttaatta tttatttaat tatataaaaa atatatgtga
     4381 taaagatgaa aatatagttg gaatgagatt atatgtagaa aaagaaaaca taaatgcaaa
     4441 agcaacatat gagtctttaa atatgtatga gtgtgattat aatatgtatg
//
LOCUS       DACULQ010000232         4469 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.234, whole genome shotgun sequence.
ACCESSION   DACULQ010000232 DACULQ010000000
VERSION     DACULQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.234"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1185
                     /locus_tag="JA154_17130"
     CDS             <1..1185
                     /locus_tag="JA154_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein BlgG"
                     /protein_id="HAT7997551.1"
                     /translation="LVLTDIEFKSVLNNILVMLSRHKYNEKEFIENYIENYRSKRSSI
                     LENEQNKEIIINSIKTFCKNLKLATSIDISNDKVFEEFLYKHISSLCKKIKLGINQSP
                     IMAQDIKIKYPFAFELANIAKKTIEKDLKIEINEDEVANIALHIGGAVERASYNNKDK
                     VFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILKIIPSYLVEYIKVIDVDFVISTVPI
                     EVGDVPVINISPILSEKEIRLIEKYIETGNIYLDLDIKSLFDSELFFTDLVFKRKEEV
                     IDFMASKLVQKGYIDEDMKKSYFEREKIATTEIGNMVTIPHGAIGKIFDNKIAIGILK
                     KPISWEISDVRLIIMLALDKDKILDYEVIFSKIYKRVDSIAKVISICENSSFEKFVNL
                     FN"
     gene            1352..2269
                     /locus_tag="JA154_17135"
     CDS             1352..2269
                     /locus_tag="JA154_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAT7997552.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            2306..4225
                     /locus_tag="JA154_17140"
     CDS             2306..4225
                     /locus_tag="JA154_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAT7997553.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         1787 a          445 c          834 g         1403 t
ORIGIN      
        1 ttagtattaa ctgatataga atttaagagt gtgttaaata atatattagt tatgttaagc
       61 agacataagt ataatgaaaa agaatttata gagaattata tagaaaatta taggagcaaa
      121 agaagctcaa ttttagaaaa tgaacaaaac aaagaaatta taataaattc aataaaaaca
      181 ttttgtaaaa atttaaaatt agctacatca atagatataa gtaatgataa ggtatttgaa
      241 gagtttttat ataagcatat tagtagttta tgtaaaaaaa taaagttagg tataaatcaa
      301 tcacctataa tggctcaaga tataaaaata aaatatccat ttgcttttga actagctaat
      361 atagctaaaa agacaataga aaaagatttg aagatagaaa ttaatgaaga tgaagttgca
      421 aacatagctc tacatatagg aggagcagtt gaaagagcat cttataataa taaagataag
      481 gttttcaaaa ctataatagt ttgtacttca ggcataggca catcaatgtt aataaaagct
      541 aaattagaga atatatttaa ggaaaaatta gaaatactaa agattattcc ttcatactta
      601 gttgaatata taaaagttat agatgttgat tttgtaatat ctactgttcc tattgaagta
      661 ggagatgtgc cagtaataaa tatatctcca attttaagtg agaaagaaat tagattaatt
      721 gaaaaatata tagaaacagg aaatatatac ctagatttag atataaaaag cctgtttgat
      781 agtgaattgt tttttactga tttggtgttt aaaaggaaag aagaagttat agattttatg
      841 gcaagtaaac ttgtacaaaa gggctatata gatgaggata tgaaaaaatc ttactttgag
      901 agagaaaaaa tagcaacaac agaaataggg aatatggtaa ctataccaca tggagctatt
      961 ggaaaaattt ttgataataa aatagcaatt ggtatattaa agaagcctat ttcttgggaa
     1021 atatcagatg ttcgtttaat aataatgtta gcccttgata aagataaaat attagattat
     1081 gaagtaatat tttcaaaaat atataaaaga gtagattcta tagcaaaagt tataagtata
     1141 tgtgaaaata gtagttttga aaaatttgta aatttattta attagatggg tagtattgta
     1201 tatacacctg taaatttgga taatataaaa ctaaataaga taaaaacgat aaatttctct
     1261 tttaattaag agaaattttt tgactttcaa ggatattaat ataaagttaa gatataaaca
     1321 taaagcaaat aaataaaaaa gaggtggcaa tatgatttat acagtgacat taaatccatc
     1381 aatagattat atagttaagc tagatgagct aaagacagga agtacaaatc gtgtaaaaga
     1441 agaatacgtt tatccaggag gaaaaggtat aaatgtatct cgcatactta aggaacttgg
     1501 aaatgataat acatctttag gttttataag tggttttact ggagaatata taataagaac
     1561 attggaagaa aaagaattga agacagattt tataaagata aaaagtggtt tttcaagaat
     1621 aaatgtaaaa ataaaggcgt taaaggaaac tgagattaat ggacaaggtc caaatataga
     1681 tgatgaagac attgatactt tatataaaaa acttgataag ctaaatcaag atgatatttt
     1741 aatattggca ggaagtatac catctacttt agatgaaaaa ctatacgaaa atataatggc
     1801 tagattagaa aaaaagaata taaaagttgt agtagatgca actaaaaatc ttcttcttaa
     1861 tgttttaaaa tacaaacctt ttctaattaa gcctaataac gacgaattag aagaactatt
     1921 tggtgttaaa ttaaattcaa ttgaagacat ggtaaaatat gctagaaggt taaaggaaat
     1981 gggagctata aatgtcttgg tatcaatggg aaaagatgga gcattgttga ttacagagga
     2041 agatgaagtg cttataagtg atgttccaaa aggaaaagta aaaaattctg taggagcagg
     2101 tgattcaatg gtagcaggat ttatatctgg gtatttgaat actgggaaat atgattatgc
     2161 tcttaaattg ggagcagctt caggtagtgc aactgctttt tcatatgact tagccaaaag
     2221 agaatacatt gataaattgg taaatgaaat atctgtaaag aagttttaaa atattaaaaa
     2281 atatatagaa aggtaattaa tatttatgaa aattatagat ttattagatg aaaagtcaat
     2341 taagttaaat ttaaattcaa aaaccaaatc agaagcaata gaagaattag tagatttggt
     2401 cgcaaatagt ggaaatttaa atgataaaga aaactacaaa aaagcaattt tggctcgtga
     2461 agaaatgagt acaacaggaa ttggagaagg tgtagctata cctcatgcta aaaattcttc
     2521 agttactaaa gcttgtatag cagcagcagt gtcaaaagaa ggtatagatt atgaatcgtt
     2581 tgacggaagt ttatcaaatc tattttttat gatagctgca ccagacggag ctaacaatac
     2641 tcatcttgaa gtattatcaa gattatctac aatacttatg gatgaagact ttagaaataa
     2701 acttataaat tcaagctctg aaaaagaatt taaggaaata atagataaaa aggaaagaga
     2761 aaaattcagt gaagaatatc aagatgagaa agtggctgaa aaaaatataa tagatactaa
     2821 agagaatgat gctaataagt atcctaaggt tttagcagta acagcatgtc caactggaat
     2881 agcacataca tttatggcag ctgaaagttt aaataaaatg gcagaaaaca aaggtgtatc
     2941 aataaaagta gaaactaatg gttcagcagg tgttaaaaat aagcttacaa aagatgaaat
     3001 agaaaatgca acatgtataa tagtggcagc agataaaaat gttgagatgg ctagatttaa
     3061 tggcaaaaaa gtcataaaaa ctaaggtggc tgatggtata cacaaagcgg aagaacttat
     3121 agacaaggct gtaaatggag atgctccaat ttaccatgga ggagatggaa gtcacaacga
     3181 atcaaatgaa gaaagtgaaa gtggatttag aaaagtatac aaacacttaa tgaatggtgt
     3241 atctaatatg ttaccatttg ttattggtgg agggatactt atagcgatag cctttttact
     3301 tgatgattat actattaacc cttcaaactt tggttcaaat acacctatag cagcattttt
     3361 taaaggaata ggagataaag cttttggatt tatgttacct gtacttgctg gatatatagc
     3421 atactctata tctgatagac cagcgtttgt tgtaggattt gtaggaggtg ctctagcagg
     3481 tgatggtggt tcaggctttt taggtgcact acttgcaggt tttatagcag gttatttagt
     3541 agaagggctt aagaaaatat ttagtgtact accagcttca ttggaaggaa taaaaccagt
     3601 attgttatat cctttacttg gaacactttt gatgggaatt ataatgacat ttttagtaat
     3661 acctccagtt acagcaatta ataatgcaat ggttggattt ttaaatggtt taggtggaac
     3721 ttcaaaaata gttttaggat tagtattagg tggaatgatg gctgtggata tgggtggacc
     3781 tgtaaataaa gcagcatatg tatttggagt tgcatcactt gaatcagggc agtttgaaat
     3841 aatggcagca gtaatggctg gtggtatggt tccgccactt gctatagctc ttgcaactac
     3901 attttttaag aatagattta ctaaagaaga aagagattct ggtaaagtta attatgtaat
     3961 gggattatca tttgttactg aaggagctat accttttgca gctggagacc cacttcatgt
     4021 tataccagct tgtgttggag gttcagcagt ggcaggagca cttagtatgt tgttcaacgc
     4081 agctcttcgt gcacctcatg gaggagtatt tgttatacca gtggttaccc atccatttgc
     4141 atatatatta gctatagctg taggtgcttt ggttggaatg atgcttttag cattacttaa
     4201 aaaaccatta aatcaagaag tatagcaatt aaaggatatt caagaactta aaatatgatg
     4261 ttttataaaa attaatatat tgctgaatta ttttaaaata aggattttat gaggctgtta
     4321 tataacgatt tgtaattgtt atcttgacag tcttttattt ttatataagt aaaataagtg
     4381 tgttttgctt tatttgtatt taaattgttt ttaagttaga atataaagaa aattatatat
     4441 tattttaata ttcactcgaa ctagaaaat
//
LOCUS       DACULQ010000233         4286 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.235, whole genome shotgun sequence.
ACCESSION   DACULQ010000233 DACULQ010000000
VERSION     DACULQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.235"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            50..730
                     /locus_tag="JA154_17145"
     CDS             50..730
                     /locus_tag="JA154_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HAT7997554.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            753..2102
                     /locus_tag="JA154_17150"
     CDS             753..2102
                     /locus_tag="JA154_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HAT7997555.1"
                     /translation="MPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDIYALQDLRLAT
                     GKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENASDIHIEPFKE
                     HLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGRVDIKLGGNLI
                     DIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINKRAGILLVTGP
                     TGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGLSFDVGLRAIL
                     RQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLLEMQIPPYLLN
                     ASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKGYFGRTAIYEI
                     LEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLEECLVVNEMI"
     gene            2273..3358
                     /locus_tag="JA154_17155"
     CDS             2273..3358
                     /locus_tag="JA154_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HAT7997556.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKAKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            3412..3771
                     /locus_tag="JA154_17160"
     CDS             3412..3771
                     /locus_tag="JA154_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAT7997557.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            3824..>4286
                     /locus_tag="JA154_17165"
     CDS             3824..>4286
                     /locus_tag="JA154_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HAT7997558.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNIS"
BASE COUNT         1853 a          388 c          699 g         1346 t
ORIGIN      
        1 attaaagaaa attatcatat aaaaataaat ttgtaaggag caatacagta tgaaaaaaca
       61 atatgtgttt ataatactag caagtactat tttaggtata cttgttggaa ccatatttag
      121 agattatagc aatgaagaaa cagtttatac aagcaaagac aagtcaataa gaaaagaaat
      181 aaaaatgaca agaaaatcta taaaagaatt aaccaaagag aaagacaagt tagataaaga
      241 aattaattca atgaaacaaa atcaaaaaaa ttctgaagaa atatcagaaa tagaaacact
      301 tagagatagt ctatcatatt cagatgtaaa gggtagtggg ataatattaa gtatagatgc
      361 tatagatgaa aaaacaggga atatagctaa tagtatagac tataataaga ttcttgtaaa
      421 agttataaat gaattaaaag taaacggagg aaaatacata gaaataaatg gtcaaagaat
      481 taatcaatat tcagaagttg tacttgcagg aagccatata aatataaatg gagttcctgt
      541 agcacaacca tatgaaataa agtgcattgg agataaggag aaactatcta attatgtaga
      601 taaaggtagt aaatatttag atagcataca aaccaattat ccaatgaaaa taagcataaa
      661 aactgaagaa aatatctcta tgaaaaaaat gaatatacct aataaattaa agtatataaa
      721 gggtgattaa gattatatta gaggaaagca gtttgccaat gttaatacca gaaaaacttg
      781 caaaaaaata taatgtattt ccagttgagt ttcaagatga taatttagtt gtagaaatag
      841 aagatgagga tatttatgct ctacaggact tgagattagc tacaggtaaa gaaattatat
      901 tgaagaagga gaaaaaagaa cttataagtg aaaagattga aaaatattat aatagctttg
      961 aacttgatga ggattatgca aaaaaattat ttgaaaatct tttagaaaaa gctataaaag
     1021 aaaacgcaag tgatatacat atagaaccat ttaaagaaca tttaatagtg agaatgaggg
     1081 tagatggaga actaaaggaa gtatcaaagt ttttagttga tgtctatcca tcattgtcta
     1141 cggttataaa attaaaggca tcaatggata taacagaaaa aaggcttcct caggatggta
     1201 gggtggatat aaagttaggt ggaaatttaa ttgacattag agtttcatca ataccaactg
     1261 tctatggtga aaaaatagtt ttgaggatac ttaatagaga tactttttta aaagacaaat
     1321 tagaacttgg tttttcagag gaagctataa aatctataaa tcgtataata aataaaagag
     1381 caggaatatt attagtgaca ggtcctacag gtagtggaaa gactacaaca gtatattcct
     1441 tattaaatga tttaaaagga ataaataaaa atattatgac tatagaaaat cctgtagagt
     1501 ataagatgga tggcataagt cagattcaag taaattcaaa agtagggttg agctttgatg
     1561 taggtcttag agcgatatta agacaagacc cagatataat aatggtcgga gaaataagag
     1621 atgcagaaac agctaagata gcagtaagag cagctataac aggtcattta gttataagta
     1681 ctttacatac taatgatgca gtatcttcca tagctagatt actagaaatg caaataccac
     1741 catatctttt aaatgcttct cttataggtg ttatatctca aaagttggtt agaaaagtct
     1801 gcaatcattg tagccatgag attatgataa aagataattt tagtggagat gtaaatacta
     1861 aggtagctgt agggtgtgaa aaatgtaatg ataaaggata ctttggaaga acagcaattt
     1921 atgaaatctt agaaattaat gatgatataa agacatgtat aagaaatatg gaggattcta
     1981 gtactattaa agatgtagca caaaataatg gaatgataac atttgaagat agttgtaaaa
     2041 gactaataaa tgaaaaaata acgacattag aagagtgttt agtagtaaat gaaatgattt
     2101 aactgaattg tatagttaaa cttcgtttgg tcagttgtct ttatttaatc tattttaatt
     2161 aagatgtttg agtataaatt atatttaagt gaaataaaat taaatgcatt aaattagaaa
     2221 atattaagtt tatttaattg taattaatat aatttggaag gtattatagt acatgagatt
     2281 atctaaaaat atattttata atttagaaaa gttttattta gtattaaata gaatcagtga
     2341 taaagaaatt agagtaattt gcaaagagat aggcatattg ctggaatctg gatgtgaaat
     2401 tacaaaaata tttgaaatca ttgagagtca gtcaagtaaa aaagcaaaga atttgcttag
     2461 tatagtatca aatcatattc aaaaaggtaa ctcaatagca gaatcatttc aaataacagg
     2521 tatattttct aaattcttta taagtatgat aaaagcagga gaaactagtg gaaacttaga
     2581 tataataatg agtgatttat ctaattatta tgataaagaa tacaaattaa aaatgaaaat
     2641 aataactatt tcaatatatc caataatact tataatatta tctatcttat caatgttgtt
     2701 tatattcgta tttgtaatac ctaattttca ggtggttttt accaacaatg gaattgaacc
     2761 acctttaata actagagttt taatgggaat atctacagtg gttacaaata acttagctta
     2821 tataatattt agttttatat tgttttcaat aggaagcatt tatttcttta taacaaatga
     2881 taatataaaa aaattgatta attcgttaaa attcaaaata ccttttataa aaaaaattaa
     2941 tcagttagta gctacaacaa gattttcaag ggcattgtcc gttttaatta gcagtggaat
     3001 acaaattgtg gaagctatag acatgtcagc tagtgttata gacaacgaat ttatttatga
     3061 aaggttaaag atttccaata attatataaa aaaaggaaat agcataggta aatctttgaa
     3121 tttagcaaat gtttttccaa aattatttat ttcaatggca aatacaggag aagaaagtgg
     3181 gaaactagat aaaagtctaa atgtaataaa taaattttat gaaaatgaat taaataacaa
     3241 aatagagcaa ataatgaaat ttatagaacc tatgctaatt gtatttatgg gattaattat
     3301 tggtactttt attttagcaa tggttgttcc tatgtttgat gcaattacta atttttagag
     3361 agaaaaataa attttattag atgaaacttt tgaaaaggag agaatattta attgataaat
     3421 ttgataaata aaaaacgaaa aggatttaca cttgttgaaa tgattgtagt agtaactatt
     3481 ttaggcgtta tatctagtat agcattagtt aagtatagta aggttcaaga aagtgccaaa
     3541 ttaaatgcag actatacgaa tgctgctaat atagtaaccg cagctagcat ggcaattaat
     3601 gatgatgaaa agacaataga ctctctaagt gtagagacat tgaaggaaaa gggataccta
     3661 aatactgttc cagttcctca gagtacatca ggtaaattcg aacttgtcat aaatgatagc
     3721 ggaacagata taagcgtaaa tataaattct aagcaatttt atccaaaata atattttata
     3781 ttgattgctg taagttttaa aattattttt atttaagaag gagatggcta taattaaaaa
     3841 aattctaaac ttagaaataa atgatgatta tataaggctt gtatttttaa gaaaaagtca
     3901 taaaaaagtt tttatagata aatcaattat aaaagagatt aactttgata taaaaaatga
     3961 tagtaattcg attgagaaga ttaaatatat agaagatgta gttagatttg taaaagagga
     4021 gattggtaaa tcaaaaatat tatataaaaa tttatacttt aacattcaaa ttcaggatat
     4081 agtcattaga aatgttgaag ttctaaatac taagaaaaaa agagacatat taagcatgat
     4141 tgaatttgaa ataacacaat acattccaat aaacataaat aactattcaa taaaatataa
     4201 agttataaat tctaaaaatg ataaattgaa tatacaagta attcttttcc caaagagtat
     4261 tattcaatta attaagaaca tttcag
//
LOCUS       DACULQ010000234         4195 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.236, whole genome shotgun sequence.
ACCESSION   DACULQ010000234 DACULQ010000000
VERSION     DACULQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.236"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..711
                     /locus_tag="JA154_17170"
     CDS             <1..711
                     /locus_tag="JA154_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HAT7997559.1"
                     /translation="SCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFNYGEERKNNK
                     QIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKVKELEYKGYR
                     FKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLNYIANLTLES
                     IRGSGLRKPLEFIPLNFKKRVLKEKIRGFKEREFYYINAYAGTFFLKGNMEDLNALYK
                     MGIGYRRTENAGMVDILK"
     gene            716..2191
                     /locus_tag="JA154_17175"
     CDS             716..2191
                     /locus_tag="JA154_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997560.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFCELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMEKNVKRIVDIVIKSN
                     IEIDEIDKLDLKQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRIDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     MNTRNLGKKMINNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGREEYKMKNYIESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2211..3227
                     /locus_tag="JA154_17180"
     CDS             2211..3227
                     /locus_tag="JA154_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HAT7997561.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYRDKVHA
                     KTSIATITYDLRRILHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKEKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEREENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEEDGKLLLPEERKFMSKELREK
                     AVKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGV
                     IETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMW
                     YLKE"
     gene            3243..4040
                     /locus_tag="JA154_17185"
     CDS             3243..4040
                     /locus_tag="JA154_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HAT7997562.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
BASE COUNT         1818 a          359 c          730 g         1288 t
ORIGIN      
        1 tcatgtaatg gcaaatttcc aaatgaaaat tcaatgttgt cagtttcctt tataaaaaat
       61 attttaaatg cagaaaacaa agattttgct aaaaacatat ttaactatgg ggaagaaaga
      121 aaaaacaata agcaaataaa ggatataaat acagccattt acataccaaa tcttataaga
      181 agcaaaaatg agttacttgt tgatggagat ataatattca atataagttt ttataattat
      241 tctatgttca gtaaattata caatggaatt ttaaaagtaa aggagctaga atataaaggt
      301 tatagattta aaataaaaaa tataaaaata cataaagaac acgagataaa agaaaatggt
      361 gttatattta aaactatgtc tcctataata gtaaagaata aagaaggtaa atatttagat
      421 gtagaagatt caaattatat agaatgttta aactatatag ctaatctaac tctggagagt
      481 ataagaggta gtgggcttag aaagccattg gaatttatac ctcttaactt caaaaaaaga
      541 gtgttaaaag aaaagattcg tggatttaaa gagagagaat tttactatat aaatgcttat
      601 gcaggaacat tttttttaaa aggaaatatg gaagatttaa atgcactgta taaaatggga
      661 attggatata gaaggacgga aaatgcagga atggttgata tattaaagta gcgaggtgag
      721 agaaataaaa gctaaatttt atagatcagg tgatattgta agagatattg gcattgttta
      781 cttttgtgaa ttactaatgg atttaaaaaa cgaactagaa aaaaatgatt ataagctaaa
      841 atttacttgt gagttgaata gaaattattt atctttagaa tctgcagaat atatagaccc
      901 taagtatata tctgattata tattagaaaa tcaacttttt aaggtattta aaaatgggaa
      961 aaaggaaacc ttagaaaaaa tatttagtaa tatagataat ttaacatatg taaattttat
     1021 tgaagaattg gataaatctt ctgcaactga gaaacaaaag gaagggatta aaaaagattt
     1081 taaaaacaga agatttccat atgttagaaa tagtggtaag tatggattaa acacgagtct
     1141 agaaaatatg gaaaaaaatg taaaaagaat agtcgatatt gtgattaaat caaatataga
     1201 gatagatgaa atagataaac ttgatttgaa acaatatgaa gaaaaagata gtgtatgtaa
     1261 tgtttgcaat ataaataaaa caacaaaact agatattgat ttaagagaac gaaaggactc
     1321 taagtataac tttttattta gaggtgcaga aaaatcaggt tttaaaagaa gtggacaagt
     1381 tgaaagtaat atttgttttg agtgtgaatt tttcaactta atgtgtcttt tatatataaa
     1441 tttaaaaaga ccaatggttt ttgcatatac gaatgattta agagaattag catttttaaa
     1501 tcataagata atgcttaaaa gaaaaatgta ttcagataaa tcattttata aaaaactttt
     1561 gcatgaaaaa ataagttcaa ttagactata taggattgat attgatacga ataaaggtat
     1621 aattctaaaa tttgactcta ttatagagta taaagaatta ttaaaaaaaa ttgaacttat
     1681 agatattata gataattata atttttcaag agagccaatg aatactagaa atttgggtaa
     1741 aaaaatgatt aataatggga atttatcaaa tttaaaagaa ttattacttg ataatataag
     1801 tgttttaagg caactaactg gaacttctcg agaaatggat tttgtatcta gttcatataa
     1861 tatcggttta tatataaaat tgtgttggat tgtagatgga agggaggagt ataaaatgaa
     1921 aaactatata gaaagtaatt taatatattc aagagtaggt aaagacttat ttaataaaat
     1981 gacagatgaa tccagaagaa aaaatttttc tatgaaagtg attcaactac ttaagtctaa
     2041 tgatagaact cagctatttc aaactataat gcatgtgtta gtttctaatg gaatcttaat
     2101 aggagaaggg tttgtagaag gtctaatgca gagttctgaa ttagaattaa atacgaatgt
     2161 aggtctattt attcaagaaa tcatgaaata aaaaaggggg aaaatttaaa atgaatgaaa
     2221 aaagagtaaa aggagtaaca ttaactatac tgacagaaag cccagttcca ctttcgtatg
     2281 accaagggta tggaaattat acaccaataa aaaaagaaca atatagagat aaagtacatg
     2341 caaaaactag tatagctaca ataacatatg atttaagaag aatactccat caagaatatg
     2401 gttggaattt aagtaatatt gtttttggaa aaaaaggaaa tatatatcca tctattaaga
     2461 aaaatgtagg atgtgtagat gaaaatggat tagagactga tgtatttggt tatttaatac
     2521 cacttaaaga gaaaggttct ataagtaagg cttctccgct tagaataata ccttttaggt
     2581 cactaaattc atatagaggt tcaacacagt tgataacaaa tagaggtttt ttaagtagcg
     2641 aatttggtag aaaatattat gatgagaggg aagaaaatga agtacttaga gatgaaaact
     2701 ttccaacaac acaagcatta gcaatagaag agactttatc agattactac gtttatacaa
     2761 taacattgga gcttgataga ataggagtag tagaagtaga agaagatgga aagttacttt
     2821 taccagaaga aagaaaattt atgagtaagg agttaagaga aaaagcagta aaagatatat
     2881 tagatgcaat tactgtgttt acaagaaaca taaaacattc atcagtacta ttaaaaccac
     2941 ttgcagtaat gggtggagct tttgataaag ttgtaccatt tttttgggat gatgtagatt
     3001 ataatgctga tagtggtgag ataaatttag agggagtcat agaaactata gaaagttata
     3061 gccttaaaga aagtaacaca atacttgcta tcaatgacag attaaaaatt tctaataaaa
     3121 aggaattaaa taagtttaat ttaagtaaat accctgtaaa agagattaaa aatctagcag
     3181 atagattgga aattggagaa gataatatgt ggtatttaaa agagtaggag gttttttgaa
     3241 ttatgaaagt attacaatgt aaacttaagc aaccaactgc tcactataga gacccaaaag
     3301 tatttcaaaa tgaatatata agtactttaa atttaccatc taagaccaca ataatgggaa
     3361 tgataacgta tttatgtgat agaagattaa actctgatat agatatagga ataattggaa
     3421 cacatcatca tagagaacta gaattttcaa gaggagagaa cattgatttt tggaatgaat
     3481 atacaaatat gagaaaaggc aaagataaag aaaagttttt gcttcaagga aactactatg
     3541 attactataa agaacataaa gctcaaaata gcatattaaa ctatgaagtt cttaaagaag
     3601 tagaactgac tatatttatt tcttgcaagg atgatgaaga acttgaattt attagaaaaa
     3661 agcttgaaag tccatgtaaa tatgcaaatt taggaagaaa agaagatttt gtaataccaa
     3721 gtgaaaaagg gtgttttgtg aaagaagttg agttaaaaga ggttatacct atgaatactc
     3781 gtgatgctat aaaagaaaat ataaaattaa agaatacata tgtaagagtt gacttaagag
     3841 ataaagagaa tgtcgagact ataataaatc aaggtgtatt gattgcatta ccttataaat
     3901 ataaagattt agaagcaaat agagatgata gagtttatga gttttgccat tatatatatg
     3961 ttgataatga tggtatatat cctaaaaata ttaaggtaaa tgtatgtatt gatacaaaag
     4021 aagtttttac atggctttaa attattttga aagataggtg tttatcatga aaaatatcat
     4081 gcaaaaagaa atattagcta agtcatataa tttaagaact aatcaacaag agcaaacatt
     4141 agttgaacat attaaggatt tatttgaagt attagaatct atattagaac taaat
//
LOCUS       DACULQ010000235         4161 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.237, whole genome shotgun sequence.
ACCESSION   DACULQ010000235 DACULQ010000000
VERSION     DACULQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.237"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            27..1334
                     /locus_tag="JA154_17190"
     CDS             27..1334
                     /locus_tag="JA154_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997563.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYYNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1420..1682
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1808..2611
                     /locus_tag="JA154_17195"
     CDS             1808..2611
                     /locus_tag="JA154_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT7997564.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2649..3260
                     /locus_tag="JA154_17200"
     CDS             2649..3260
                     /locus_tag="JA154_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAT7997565.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3292..3963
                     /locus_tag="JA154_17205"
     CDS             3292..3963
                     /locus_tag="JA154_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAT7997566.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1654 a          396 c          769 g         1342 t
ORIGIN      
        1 acataaaagc aaaggtggga ataaaaatga aagaaattgc tgatgctata acatcagagt
       61 atttaagcta tggtatcaat gaagatatta gtatctatga aggtagattt tgcatatatt
      121 tagataaaaa atatagatgt aatgggaaaa tttattataa aatgacacct ccaatatcta
      181 taagttttaa agcagacata ggttgtgttg aagagataga caatgaagat gacaatttag
      241 ctttagatta tgataatgct attcttgaag ttcatggata taaaattatt tctataacaa
      301 taaatacttt aagtgaattt agtgtagaag gttatatcaa tgatgactgc ataaaatcta
      361 agaactccta tgttgaatat gtagatttta atataataaa cttagataaa atacctggaa
      421 aactcattaa atacaatgat aaggtatacg caggtagaat tgaatttgat ataaatgatt
      481 atgttgtaac tatagacaag cgatatgatt atagaaaaga attaaaatca gaattaaagt
      541 ctaaatctgg agcaataata actcatatag gtaggattag aagaaaagat ggtagaattt
      601 ttagaacaaa taatacaata aatcttttag ataggatatc aacagctttg agttttatgt
      661 gtggaagata tgtagggttt tgtttggcca aagggtatag aagtggtaat gaagtttata
      721 gaatatggaa tgaaaatcaa atatctcctt ttagatatgt accaacatgg tcagatacac
      781 tttcaaatta ttataatatg gaaaaatata taagccttat gtgtaaaaag ctagaagatt
      841 tttattatgg ttctgctata aagagtgttg ttgattggta catagaatct cttggaagtg
      901 ctaccatgga aaacaatata atatctgtac aaattgcact tgaaactttg tcatatgtaa
      961 ttctagtgga acaaaataag atacttacag atgaagtatt tgactgtaat ttggcatcta
     1021 aaaatataag actcttactt gatacatgta agataccata tggtaaacat gaattaaata
     1081 tatttgataa tataataaaa aataaatttg atgatggagt ggatttagtt atttatttaa
     1141 gaaatagcat agttcatcca agtagaaaga ctcatagagc tgtgttagag gttgaagata
     1201 tttggaatat aatttcaata ggaacgagat atattgaact tgttttactc tttattttag
     1261 gttatagagg agaatattca aacagattag tagaaagatg ttatggtgaa gtggaagttg
     1321 taccttggaa ctagattttt atatttattt taaaatatat attgacatat attgccttat
     1381 gctataaaat caatatcata ataataattc agaataacat taaatgctaa gaaaagaaat
     1441 agtaaaagta gttaatctta aacagagagt ttccgattgg tgagaggagc aatagatttt
     1501 actttgaaca catctttgag caggactctg aaagagtaga aatactttat taggagattt
     1561 ccggaatcct tgcacccgtt atagtgctag agtataataa ttttattgta cttaaagagg
     1621 ttagtgtcgt gaggcattaa tgaacagagg tggaaccacg agagttaact cccgtcctct
     1681 agcagaagct agaggcggga gttttttaat ataatttttt aagcgcgcag agaattatat
     1741 tgcaatagat acacttttta aaaaataatc aaaatatttt aaaaattaaa aggcgaggga
     1801 gaatattatg aaaaatatat taaaaaaagt aggaatattt acaattatgt taggtttatt
     1861 aggtggagtt gtaggttgtt caaaaccaga taatgaaaaa gataaggatg cttcaaagga
     1921 atctaaaaaa gaagtagttg tagggtttga taatacgttt gtaccaatgg gatttttaga
     1981 tgaaaaggga gatacagtag gatttgacgt agacttagct aaagaaacat ttaaaagact
     2041 tggaatggaa gttaagtttc agcctataga ttggtcaatg aaagaaactg aattaaatga
     2101 ttcaaaaact gtagatgtat tatggaatgg atattctata acagacgaaa gaaagaaaat
     2161 agtatcatat acagaaccat atttacaaaa taagcagata atagttactc taagtgactc
     2221 aaaaataaat tcaaaagctg atttaaaaga taaagaagtt ggaacacaac aaggttcaac
     2281 agcacttgat gcagtggaaa aggataaaga ttttatgaat agcttaaaag gtggagcacc
     2341 agttctttat gatacttatg ataaagcact tagagattta gagataggta gaactagtgc
     2401 agttgttggt gatgaagttt taattagata ctatatggga caaaagggtg aagacaaata
     2461 caaggtatta aaagacgatt ttggattaga agattatgtt gtagcaactt ctaaagaaaa
     2521 tccagaactt tgtgagaaaa taaatgaaac attaaaagaa atgaaaaaag atggaacttt
     2581 tgacaagatt tatgataagt ggtttaaata gaagataagt tagctgttaa gaagggggca
     2641 ctaaaggcat gttacaaggt ttagagatag ttatagcaat gttttgtata acattgatag
     2701 tatcaattcc attggggatt ggagttgcat ttttaagatt atcaaagaat aaattagtaa
     2761 gtggtattac acaatgttat atattgataa tgagaggtac acctttactt ttgcaaatga
     2821 tagtcatatt ttacggatta cctcttttag gaattgtatt tgacagattt acagctggag
     2881 tagtagcttt cttcttaaat tatgcagcat attttgcaga aatatttaga ggtggaatac
     2941 agtcaatcga tagaggacaa tatgaagctt caaaagtact aggatttgat aagtttacta
     3001 tgtataagag agtaatcttt ccacaagttt ttaaaagaat actagcacct atttcaaatg
     3061 aagtaatcac attagtaaaa gatacatctt tagtatatat attaggatta aatgatattt
     3121 taagaatatc tcaaatagca atgaacaggg aagcaagtct gttaccatta tttgaggcag
     3181 gagcgatata tcttatattt gtagctatac ttactaaggg atttgaatta cttgaaaaaa
     3241 aatattctta ttatagataa agttacaata ttataggagg aggtttttta tatgttgaaa
     3301 atcaaaaact taaataaatc atttaaaaaa aatagagtac ttaaagatat ttcttttgag
     3361 ttagaagaag gtcagattgg agttttactt ggtaagtctg gtgcaggaaa gaccacaata
     3421 ttaaggtgta taaatggtct tgaagaattt gatagtggag agattataat agataatgag
     3481 gtaataaaaa ataagagaga tatggcaaaa atacgaggaa aaataggtat ggtttttcaa
     3541 aatttcaact tatttcctca tatgacagtc cttgaaaata taattgaatc cccagttaat
     3601 gtttttaaag ttcctagaaa agaggctgaa gagagggcta gagaactttt aagattagtt
     3661 gatttagaag ataaattaaa ttcttatcca tttgaattgt caggaggaca gcaacagaga
     3721 gttgctatag ctaggtcatg tgcattgatg ccaaaagtcc tttgttttga tgaacctact
     3781 tctgcacttg atattgatac tattcaaaga gtagtcaata taatgaacag acttaaagac
     3841 aaaggtatga caatattaat aattacacat gatgttgtct tctcaaataa tgttgcagat
     3901 aaaattataa gtataaaaga tggtatagta gaaaatgttc aaataaaaga aaaaattgtt
     3961 tagagtttta taaatataaa ctagggtaga attttataat ataaataaag ggcaagtaaa
     4021 atcaaatata ctatgccctt ttgttatctc aaaatttaat cttatatttt atgtttaagt
     4081 tacattataa tttataataa aaataaaagg actatttaaa tatataatgt gaataatgtt
     4141 taataatttg aaatatgata a
//
LOCUS       DACULQ010000236         4159 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.238, whole genome shotgun sequence.
ACCESSION   DACULQ010000236 DACULQ010000000
VERSION     DACULQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.238"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            71..1765
                     /locus_tag="JA154_17210"
     CDS             71..1765
                     /locus_tag="JA154_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAT7997567.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2097..2207
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2311..2682
                     /locus_tag="JA154_17215"
     CDS             2311..2682
                     /locus_tag="JA154_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HAT7997568.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2779..3726
                     /locus_tag="JA154_17220"
     CDS             2779..3726
                     /locus_tag="JA154_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAT7997569.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3830..4147
                     /locus_tag="JA154_17225"
     CDS             3830..4147
                     /locus_tag="JA154_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HAT7997570.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT         1724 a          387 c          780 g         1268 t
ORIGIN      
        1 agtattacat agtacatgta aatctatatt tataaataca ataaagtaaa gttgtaaagg
       61 agtgctttaa atgaagtttt cagagtttaa gtatgaaaga cccaattata atagtatgaa
      121 aaaagagttt ttgagttgtg tagaagatat aaataattct cgtaattata aagagcaaca
      181 aaaaaatata cataagatta atttattaag aaataaaata gaaacgttgt caaatatagc
      241 ttcgataaga tatagtacag acacatttaa taaattctac aaagaagaaa aaaattattg
      301 ggatgaatat atgcctttgt atgaagaatt aaattcatat ttttataatg ccattgtaaa
      361 ttcaaaattt aaatatgatt tgataaaaga gtttggagag caatttttca caatagtaga
      421 gtattcttta aaaagttttt ccaaagaaat aataagtgaa ttgcaggaag aaaataaatt
      481 gtgttcagag tatactagac ttttagcttc agctgagata atgtttgatg ggaaaataag
      541 aaatttatct ggtatgggga aattcatgta ttctaagaat agaaaaacaa gagaattagc
      601 aaacaaagca tattataatt tctttgaaga aaatgaaact aaatttgatg acatatttga
      661 taaattggtt aaacttagag ataagatgtc taaaaaatta ggttttgaag attttgtaga
      721 gcttgggtat gttagaatga tgagaagtga ctacagagaa tatatgataa aaaatgttag
      781 aaaacaagta ctaaaatatg ttgttccaat ggcaaatgag ctttatgaaa aacaagctaa
      841 aagaattggt ctagaatatt taagttacat agatgaaggc gtggaatttt taacaggaaa
      901 tgcaagttta aagggagatt caagatacat aattaaaaat ggtaaaagga tgtattctga
      961 actgtcaaaa gaaacaaatg aattttttga ttttatgtta gaaaatgaac ttatggattt
     1021 agaaacgaag aaaggaaaag gagctggagg gtattgtact tatataccag attataagtc
     1081 accattcatt ttttctaatt ttaatcaaac tgctgatgat atagatgtat taacacatga
     1141 ggcaggacat gcatttcagt tgtatatgtc tagatggatt gatatgccag aaattaattt
     1201 tccaacgctt gatagttgtg aaatccattc aatgagtatg gaatttataa catggccgtg
     1261 gatggattta ttttttaaag aagatacaga taagtacaaa ttcactcact tatcatcttc
     1321 aattaaattt ataccttatg gagtaattgt tgatgaattt caacactata tttacaaaaa
     1381 tcctaatgta gataagtcaa aacgaaaaga aatctggaga tttttagaga aaaaatactt
     1441 acctcataga aaatatggtg ataattcttt tttagagaga ggttgttggt ggtttaaaca
     1501 aggacatata tttaaaaatc cgttttatta tatagattat gtattagctc aaatttgtgc
     1561 gttacaattt tggaaaaaaa tgattcaaga tagagatgca ggatggaaag attatataaa
     1621 tatctgtaaa gttggaggaa caaaatcatt tttagatatt gtgagtatgg gaaatttgta
     1681 ttctccattt gatgatggct gtattgagtc tattataggt gatgttaaat cttggtttga
     1741 tgaaataaat gacagcaaat tataaaaaat tatgagaatt ttattgtata aaagtgaaaa
     1801 attaaaaaat atataaaaag tgattatttg ttgtgaaaat agtcttgttt ttaactaaat
     1861 tgataaaata aactaaaaaa gatttgtact taaaaaaaaa agaaaataca agaaatatgc
     1921 aatatcaaca tttggaaaaa aaagtaaaaa tgtatacaaa atacatataa aaaaactatt
     1981 gcaaatttta tagaatgttt atatacttaa gtcaacagag atagaaaaaa gctatttata
     2041 gaaaacaaag gataaattgc ttaattttaa attttctgaa aaaaataata atccggatga
     2101 agagtatggg agactgcttt ataaaaagcg accgaaggga caagtttaat cttacttacc
     2161 caaaagatta gatgaaattc tcaggtaaag gtgactgtac ttggacgggg ctctgaaaat
     2221 gattttaaca gggaagacaa attgggagat ttgtttttcc tgttttttat ttatcaagta
     2281 ataaataaaa gtttgaaaga gagtaaaaaa atgataataa ttacgaataa tccaaaagta
     2341 aaagaagaag ttcaaggcag agaagttttg tttaaagaca caacttatat tggaatctta
     2401 gaagcaagta gagatttgat acatgaaggt tatgaacttt tatctcatcc actctatggg
     2461 agtgtaaagc caaatgaaac accttacagg actgttatac taaagaaagg aaatcgtttg
     2521 gatataaact ctttaacctt aattgaagaa gcaatcatca ctgcaagtaa atttcagaat
     2581 aataaaaaga ctccaaaatg gacagaaagt gttcaagatg attttagagt aattgattat
     2641 gatatatttt ataatacaat acaaagaatg cagtatgaat aaaaaaaaat tgttaaaaag
     2701 ttattaaaac agcctaaata ttaaatctaa tttaatatta aataaaatga gaaatttaat
     2761 tggggaggat aagtcaaaat ggaaaatgtg tatgacttag taataattgg ttcaggacca
     2821 gcagggcttg cagcaggtct ttatggagca agagcaaagt taaaaactct aatcctagaa
     2881 aaagacaaga ctggtggaca aattgtaata acacatgaaa ttgcaaatta tccagggtca
     2941 gttccaaatg caacaggacc aagtttaata gctagaatgg ttgaacagtg taaagagttt
     3001 ggagcagaaa tgctaagaga caacattgtt gatactgagc tagatggaga tataaaagtt
     3061 ttaaaagggg aaaaggcaga atatagagca aaagcagtaa taataggaac aggagcaaca
     3121 ccaagaaaga ttggttgtcc tggagaaaaa gaacttactg gtaaaggggt ttcatattgt
     3181 gcaacttgtg atgcagattt ctttgaagat tttgaagtat ttgttgtagg tggaggagac
     3241 agtgcattag aagaagcaat gtatttaact aaatttgcta gaaaagtaac aattgtacat
     3301 agaagacaag gatttagatg tgctaagagt gttgaagaaa aagcaaaagc taatccaaag
     3361 atagagtttt tattagatac agtaattgaa gaaatcaaag gtgatggaat attagaatca
     3421 gttgtattta aaaataaagt tactggagaa actcatgaat actttgcaga tgaagaagat
     3481 ggaacaatgg gagtatttgt gttcgttgga ttagatgctc aaactgattt attcaaaggt
     3541 aaagttgata tggatgaaaa aggttacata ataactgatg aagatatgag aactaacata
     3601 ccaggcgtat ttgcagcagg agattgtaga tctaagactc taagacaggt tgtaactgca
     3661 actaatgatg gagcaattgc atctatagta gctgagaaat acattgatga aaaatttggg
     3721 aactagaatc tgtaaattaa gaaatagtgt ctgtaaattt aatgaataat atttaaaatt
     3781 aaaattaata tttaaaatta aaataaaaat ttcaaggggg aataagaaaa tgttagattt
     3841 agataaggct acatttgaag aagaagtttt aaatgcagaa ggttttgtat ttgttgattt
     3901 ttggagtgaa ggttgtgagc cttgtaaagc tttaatgcca gatgttcata aattagctga
     3961 gacttatggg gataaaataa aattctgtaa gatggataca acaaaagcta gaagattagc
     4021 tataaaacaa aaagtattag gtcttccaac tatggctata tacaaagatg gagaaaaagt
     4081 agacgaagtt actaaggatg atgctacagt tcctaatata gaaaacatga ttaaaaaata
     4141 tctttaatat gttgataaa
//
LOCUS       DACULQ010000237         4126 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.239, whole genome shotgun sequence.
ACCESSION   DACULQ010000237 DACULQ010000000
VERSION     DACULQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.239"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            159..1757
                     /locus_tag="JA154_17230"
     CDS             159..1757
                     /locus_tag="JA154_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAT7997571.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1992..4073)
                     /gene="bclA1"
                     /locus_tag="JA154_17235"
     CDS             complement(1992..4073)
                     /gene="bclA1"
                     /locus_tag="JA154_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HAT7997572.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGVTGEIGPTGVTGPTGVTGSIGPTGATGPTGATGVTGEIGPTGATGPTGNT
                     GVTGATGPTGNTGVTGVTGATGPTGATGVTGEIGPTGATGPTGEIGPTGATGPTGATG
                     VTGEIGPTGVTGPTGEIGPTGATGPTGVTGSIGPTGATGVTGEIGPTGPTGVTGSIGP
                     TGATGPTGNTGVTGVTGPTGATGPTGVTGEIGPTGATGPTGVTGEIGPTGATGPTGPT
                     GNTGATGSIGPTGVTGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNN
                     GDAITGWQTIIGNSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFP
                     ILGGKDLAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATL
                     TIFRIADTVMT"
BASE COUNT         1189 a          756 c          654 g         1527 t
ORIGIN      
        1 taatttttat attaaatttg tagtatttat gtaagaaata aattttatat aatatttttt
       61 cactaaattt ttttcttttt acaacaaata ctcactacct tttgatataa tatttttatc
      121 aattattatt atattcaagt tttgaaagga gtattaaaat gagtttatta gttttcggac
      181 ataaaaatcc agatacagat tcaatatgtt ctgcaatttc attagcacat ttaaagaaca
      241 aactaggaat aaaagcaaca gcttatgctt taggagatat cagaaaagaa gctaaatatg
      301 ccttggatta ctttaaggta gatgcacctg aaattttaga taatgttaga atacaagtta
      361 gagatttaaa ttatgataaa gtagtacctt taacaccaac ttcatctata ttagaggctt
      421 ataaccttat ggatgaaaaa aatgttaaaa cactacctgt aacttatgat gatggaactt
      481 ttgctggtat cattactatg aaagaaatag ctaaaaatct acttcatcaa cactttacaa
      541 ctatacacac atcactatca aacatttgta aaaaccttaa tggtactatt cttgtggact
      601 gtggtgaaga tataaaaggt agagtagtca ctttatcatt tggaatggag actctaatag
      661 agactttaaa agaaggagac atagctattg ttggagatag atatgatagt attgaatatg
      721 ctattgatac aaaagttaaa cttttaatac ttactaatca tacagagatt tcaaaagatt
      781 tgctagctct tgctaagata aatggagtgt ctgttgtttc tgttgaaagt gacaattata
      841 aaacttctaa tgtaataaat caatgtagtt tcttagactc tatagaagct acagaaaatt
      901 taattacatt caaagaagat gattatatgg atgaaataaa agagattatg ctagaaacaa
      961 acttcagatc ttatccaata ttagatgatg ataataagtt tttaggttta gtttcaaaag
     1021 gtcatctatt aaacccatct aaaaagaatg ttgttttagt tgaccataac gaatatgctc
     1081 aaagtgctga tggaattgaa caagcaaaca tagttgaaat aattgaccat cataaacttg
     1141 gcggtatttc tactgatgtt cctatgtcat ttagagttat gcctgttgga tgcaatagta
     1201 ctataattta tcaaatgtac aaagaaaata atgttgagat accttatgaa atagctgggc
     1261 ttcttttatc tgctatatta tcagatacat tattatttaa gtctccaaca acaactgata
     1321 tggataagaa agcttgtgaa gaattaagta aaatagcaaa agtagacatg gaaaaatacg
     1381 ctatggatat gtttaaatgc ggtacttctt tagatgaata tactatagaa gaaatagtaa
     1441 atatggactt taaagaattt aatatgagtg gacatagagt tggtattgga caagtattta
     1501 ccttagacat agactctata tttgctaaaa aagatgagtt tttatcttat ataaattcga
     1561 ctgattatga taaattaatt ctttcagtta cagatataat caaagaaggt tcttatttaa
     1621 tctataaagc agaagatagc ttaattgcta acgcatttgg tgttgaaggt gttcaaggca
     1681 catttgcacc aggtgttgta tcaagaaaaa aacaattagt ccctaattta actactgcaa
     1741 tcaaaaattt taaataatat aaaattttta aataacgaaa actgtctcaa atctatattt
     1801 gagacagttt tttattatat atatattgat aataaagtta aataaaaatc cataaattta
     1861 aaatttttat gaatatattt tattattatt gttattttat tcattctaaa ataggtaaaa
     1921 ctcaatatat aaaaatactt ttgtcattta aataaaacct aaatagtttt taagattatt
     1981 ttagacacgt tttaagtcat aacagtatca gctattctaa atatcgtcaa agtagccgca
     2041 gttcctgctg attgcccatt tcgtatccct actgttgtag atgaaaagtt atttattgta
     2101 aaagtagtcc caccaaataa aaaaccagca aaatatccag aaagtactcc tccgcctcct
     2161 ggctcagtag taagccctgc caaatctttt cctcctaaaa ttgggaatag gatagcgaaa
     2221 gaatattgat ttatacttga tgaacctggt tgtaatgcta ctgaaactga tatataatac
     2281 acaccatttt cttgtactgt aaacgtacca tttgcatcta ctgttatact tgaactattt
     2341 cctattattg tttgccaacc tgttatagca tctccattat ttagtgtttg tggagatggt
     2401 gcatttacga ggaagttagc aactggctga gaggaattgc ctgttgctcc tgttggacct
     2461 gttgggcctg ttactcctgt cgctcctgtt gctcctgttg gacctgttac tcccgttggc
     2521 cctatacttc ctgtcgctcc tgtatttcct gttgggcctg ttggtcctgt tgctcccgtt
     2581 ggtcctattt ctcctgttac tcctgttggg cctgttgctc ccgttggtcc tatttctcct
     2641 gtcactcctg ttggtcctgt tgctcccgtt gggcctgtta ctcctgtcac tcctgtattt
     2701 cctgtcggtc ctgttgctcc cgttggtcct atacttcctg tcactcctgt tgggcctgtt
     2761 ggtcctattt ctcctgtcac tcctgtcgct cctgttggtc ctatacttcc tgtcactcct
     2821 gttggtcctg ttgctcctgt tggtcctatt tctcctgttg ggcctgttac tcctgttggc
     2881 cctatttctc ctgtcactcc tgtcgctcct gttgggcctg ttgctcccgt tggtcctatt
     2941 tctcctgttg ggcctgttgc tcctgttggc cctatttctc ctgtcactcc tgtcgctcct
     3001 gttggacctg ttgctcctgt tactcctgtt actcctgtat ttcctgttgg acctgttgct
     3061 cctgttactc ctgtatttcc tgttggacct gttgctcccg ttggtcctat ttctcctgtc
     3121 actcctgtcg ctcctgttgg acctgttgct cctgttggtc ctatacttcc tgtcactcct
     3181 gttgggcctg ttactcccgt tgggcctatt tctcctgtca ctcctgttgg gcctgttgct
     3241 cccgttgggc ctatacttcc tgtcactccc gtatttcctg tcggacctgt tactcctgtt
     3301 ggtcctatac ttcctgtcgc tcctgtactt ccggttgggc ctgttactcc cgttggccct
     3361 atacttcctg ttgctcctgt atttccggtt gggcctgttg gtcctatgct tcctgtcgct
     3421 cctgtacttc cggttgggcc tgttactcct gttgggcctg tacttcctgt cgctcctctt
     3481 ggtcccattg gacctgtaat tctaattgga agacactcac atttaggata acaattatct
     3541 ataattctat cttcaaatgt tacaagtata tacggtttgt tacaacctct actagatgta
     3601 aattcaagaa tctgataagg gtaatttaaa gataaagcta acccataatt aggatattta
     3661 ttcattgccc atgcttttat agttcccgta atatttaaac aaatatagtt ggatgtatca
     3721 cttatcccaa ttggtaaata tcccctgata ttttccatag acactctatc attccatgtt
     3781 atagttcgta aattataata ttcacttatt cttttaattt caatatctac tttttcttta
     3841 aaaatatgat tcttatttga atcaatatga ataaataact ctgcttttaa tatacttttg
     3901 tcttttaata tatctggtat tctcacttta aaaaaagtaa tcaacttttc ttttacaaaa
     3961 ctatttgaac atttacttcc tattaagcaa taatctaaat tactaaagtt tttatctgga
     4021 tattttttag atataaaagt atcatcattt aaataaagta taatatttct catttataat
     4081 ccctccattt tgggtctata tcatactatg tttaaaatac atgctt
//
LOCUS       DACULQ010000238         4121 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.240, whole genome shotgun sequence.
ACCESSION   DACULQ010000238 DACULQ010000000
VERSION     DACULQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.240"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            157..2256
                     /locus_tag="JA154_17240"
     CDS             157..2256
                     /locus_tag="JA154_17240"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089513.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAT7997573.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2268..3239
                     /locus_tag="JA154_17245"
     CDS             2268..3239
                     /locus_tag="JA154_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase"
                     /protein_id="HAT7997574.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3315..3941
                     /locus_tag="JA154_17250"
     CDS             3315..3941
                     /locus_tag="JA154_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997575.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1644 a          447 c          767 g         1263 t
ORIGIN      
        1 actgactata tgtagtgctt ttgtttttga aaaatactta ttgcatttct ataattatag
       61 attaattttg ggatttcaac tgataagtac caaaagaaat aataagcaaa ttaagtgaaa
      121 ttatggcaca agattatgtt aaaaggacgg taaataatga attggataga gcttaacaat
      181 caagtgataa ttaaaaatga acaaggcaag taccaattgg aaaaggataa ggaagcctta
      241 agtagttaca tagaagaatt tatacagcca aagttgagaa aatttaataa cctagaagaa
      301 agattgaagt atttaataga tgaaggttat tattcaaaag aagtaataaa tcagtatagc
      361 atagatgtta ttaaaaatat atatgatatg attgataaac ataagtttga atttcaatca
      421 tatatgagtg caaataagtt ttatcaaaat tatgcattaa agagcaatga tggaaaggaa
      481 atattagaaa catataatga taaagtgtta attgtagctt tgacactagg aaatggtgat
      541 aaagatttag ctttaaactt ggcagataaa atagtaaaac aagaatttca accagctaca
      601 cctacttttt taaatgctgg aagaaaaagg gctggagaaa tggtatcatg ttttttacta
      661 tctgtagaag atagtacaga aggtatatca tatgctatat cttcatcaaa tcacttatca
      721 aagattggtg gaggagttgc actaaattta tctaaactta gagcatctgg cgaatctatc
      781 aaagatatag aaggtgcagc tggaggtgtt gttggagtag ctaaaatgtt agaacaatca
      841 tttagttact ttaatcaaat gggagctaga caaggttctg gtgcggtgta ccttactgta
      901 ttccatcctg attttgaatt gttgatggat actaaaaaga taaatgcaga tgaaaaaata
      961 aggcttgcta cattatcatt aggagctata ataccagata aatttatgga gttggcagaa
     1021 aaaaatgaag tagcatatgc attttatcct catactgtgt ataagaaata tggagtttct
     1081 cttgatgaga tagaaatgga tgagtggtat gataaattgg ttaatgatag tgatataaga
     1141 aaaaaagaaa taaatcctag gcagatgttg acaaaaatag cacagatgca acaagaaagt
     1201 ggatatccat atgtagtgta catagataca gctaatagag aacatacatt aaaagatgtt
     1261 ggaattatta aaatgtctaa cttatgttgt gagatatttc aatatcaaac accttcagaa
     1321 atagaggggt atggaggaaa aaatgaatgg ggacaagata taagttgtaa cctaggttct
     1381 ttaaacatag ctaatgtaat ggataataaa actatagaaa gtacagttga aacagcaata
     1441 agagcactgt catttgtagc agatagcaca gatataaaac cagtgccaac ggtaagcaat
     1501 agtaacagca agtctcattc tataggatta ggagctatga atttacatgg atacttagta
     1561 agagaaaata ttttatatac ttcagatgat gctatagatt tttctaatgt attttttgca
     1621 atggttagat actattcaat taaagcttct atgaaaatag ctatagaaag aaatcaaact
     1681 tttgaaggat ttgataaatc agaatatgtt aagggaagaa atagcaaggt tttatcaaaa
     1741 tactatgaac aatcttatct acctaagtca gaaaaggtaa gagcattatt tgaagggatt
     1801 tatataccaa caaaagaaga ctggacaaaa ttattggatg aggtaaaaga aaaagggata
     1861 tataatgcat atttaatggc agtagcacca actcaaagca ttagttatgt tcaaaatgct
     1921 acatcaagta taatgccaat tactgagcca gtagaagtaa gaacatatgg agactctact
     1981 acaatatatc caatgccatt tttaacaaat gataatatgt tgtattatca atcagcatac
     2041 aggatggata tgagaaaggt catagattta gtggcaacag ttcaaaacca tgttgaccaa
     2101 ggtatatcaa caacactgtt tgttacagat gaaaagacaa ctagagatat agctagacat
     2161 tacatatatg cctataaaaa aggtttgaaa agtttatatt acacaagaac aaagatgaca
     2221 agagatactc atgagtgtct agtgtgttca gtatagggag agaaattatg acatttaatt
     2281 tacataaaat acataatgca gttaactgga atagagaaga agatggattt acacaagctt
     2341 tttgggaaca aaatgtaaaa caattttggc ttcctgaaga aatatctgtt tctaaagaca
     2401 taaaggtatg gaatgaacta agtaataaag aaaaggaatt atataaaaaa gtattgggtg
     2461 gtttaacatt actggataca aaacagggga ataatggaat accatctatg atgagtttaa
     2521 cagaaaacct acaaagaaaa gctgtcttgt cttttatggg gactatggaa gaaatacatg
     2581 caaaaagtta ttcttctata tttatgactc tactttctaa tttagaaata gatgaattat
     2641 ttgaatggat agaaacagaa ccaacattac aaagaaaggc tgatttagtt ttagctcaat
     2701 acgaaaatac tacaaatcaa gagggattat acctatcaat ggttacaagt gtatttttag
     2761 agagtttctt gttttattca ggattctttt atccattgta tctatctggt caaggaaaga
     2821 tggtagctag tggagaaata atatcgctta ttcttagaga tgagtcttta catggtaagt
     2881 atataggatt gctagcacaa gaaatatacg attcatttga taaaatggat aaggagatgt
     2941 tagaagagaa gatgtattct atactttata gccttatgga gaatgagata gagtatacaa
     3001 atgttatata cagagaaagt gggcttgaaa aagaggttgt gaacttttta aaatacaatg
     3061 caaatagagc tttagaaaat ctagggtttg agaagctata tactgtagat gctataaatc
     3121 caattgtatt aaatggtctt agcactgaaa ccaaaacaca tgattttttt tcaactaagg
     3181 gtaatggata ccaaaaagga gtgtatgagg aattagagga tgaagatttt ataatataat
     3241 ttataattat gacacactgt tgtcactttt agcgtgataa tatcaatgta taaaatatat
     3301 atggaggtat ttttatgaaa tatgaatgga gaaaaagaga taaagagtta tatttaccta
     3361 aaaagaatcc tgtaatagtt gaaataccag aacagaaatt tatcatgtta aaagggagtg
     3421 gagacccaaa ttctaaagaa tttactgaag caataggagt actttattct ttggcatatg
     3481 caataaaaac catgcctaaa aaaggaattg taccagaagg gtattttgac tatacggttt
     3541 ttcctttaga aggtatatgg gataagggag aaaaaagtaa aggcttagat gtacttatta
     3601 aagaagattt aatttacact ataatgatac gtcagccaga ttttgttaca caagacctgt
     3661 tggataaagc tactgatata gtaaagaaga aaaaacctca taatcttctt gaaaaagtta
     3721 gatttgggtc tattaatgaa gggctatgtg ttcaaatgct tcatataggt tcatatgata
     3781 atgaaccaga aagctttgac attatgaacg aattttgcaa aaaaaataaa cttataagaa
     3841 aatcttacac acatcgtgaa atttatataa gtgatgctag aaaagtatcg cctgaatcat
     3901 tgaagacggt tttacgtttt gaagtagaaa gggaagtata aatattttga ataatttata
     3961 agagttataa aaagtgaagt ctgttgagac ttcatttttt gatgcaattt taaaattaaa
     4021 tttgatgttt cataatattt taaaaacttt ctacttttat tagtgtgaat aactgttgct
     4081 attaaataat aatactaatt atataagtat tattatataa t
//
LOCUS       DACULQ010000239         4000 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.241, whole genome shotgun sequence.
ACCESSION   DACULQ010000239 DACULQ010000000
VERSION     DACULQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.241"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            327..1235
                     /locus_tag="JA154_17255"
     CDS             327..1235
                     /locus_tag="JA154_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAT7997576.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1325..3148)
                     /locus_tag="JA154_17260"
     CDS             complement(1325..3148)
                     /locus_tag="JA154_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAT7997577.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3363..3602
                     /locus_tag="JA154_17265"
     CDS             3363..3602
                     /locus_tag="JA154_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997578.1"
                     /translation="MEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIED
                     VLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3750..3941
                     /locus_tag="JA154_17270"
     CDS             3750..3941
                     /locus_tag="JA154_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HAT7997579.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1451 a          530 c          655 g         1364 t
ORIGIN      
        1 aaggaggtat taaatatggg aatagtactt ataacagcag cattagtttt ggttatgtat
       61 ggactttcaa ttctatcata tatatcagaa agcaatatat tagaagacac tgtaaatgat
      121 aaccaataag gttttgaatt atataaataa gtgagaactg taaataaaag ttaattttta
      181 tttgcagttt ttttatatat atgtaaaata aagttaatta ataaaaaata atgttttgtt
      241 tatagtatat atggataata atagtaaagt ataagaatga tgagaataat atataagaaa
      301 taataatata aagagaggta aacgttatgg tagatatcat tgtaattggt gctggaccag
      361 caggtttaac ttcagcaatt tatgctatga gagcagggtt aagcgttaca gtatttgaaa
      421 aaaatattta tggaggtcaa gttgcaagta cctctgaggt agaaaattat ccagcagttc
      481 aaaagatttc tggggtagaa ttttctaata atatatacaa tcaagcagtt gctcaaggtg
      541 ttgatattca gtttgatgag gttgaagaga taaatttaga gggaaaagta aaggttgtaa
      601 aaacttcttc aggtgaacat aaagcaaagg ctgttattct tgcaaatgga gttgaaagac
      661 gtaaattagg ttgtgcaggg gaacaagaat ttacaggtag aggagtatct tactgtgcaa
      721 catgtgatgg agcctttttt aaagataaag aagttgctat agtaggtgga ggaaatactg
      781 cactagaaga tgctttattt ttagctaata attgtacaaa agtgtattta attcacagaa
      841 gagatagttt tagaggtgaa gaagtgttgg agaaatctgt gaaagcaaga gagaatattg
      901 agatacttta cagtcatggt gtagaaaaaa tagaaggaga aaagactgta tcgaaaatag
      961 aagtaaaaaa cttaaagact gaagaaaaaa gaactataga tgttagtggt atttttattg
     1021 ctataggtct taaacctaat aataaaatgt ttgaaaatgt tcttgatttg gatgaaggtg
     1081 gatatataat ttcagatgag tcttgtacaa catcagtaga aggtgtatat gtagcaggag
     1141 atagtagaac taagttttta cgtcaaataa taacagcagc atcagatgga gctattgcag
     1201 ccgttcaagc agctaattat ataaatgttg aataatataa atttcaaata aaaatagcca
     1261 tataaactga aataattttt cagtactata tggctatttt gttaattaga ggtaaatgta
     1321 cgttttattt accttgtcta gatttgttat atcttaatct atcatgttca tttaagaatc
     1381 tttttctaag tcttatagaa tcaggtgtaa tttcaactaa ttcatcatct tctataaact
     1441 ctagagcttc ttcaagagta aatattctag gaggagataa ttttatagca tcatctgaac
     1501 cagaagcacg tacgttactc attttcttgt ttttacaagg attgactacc atatcatctt
     1561 ttctggaatt cataccaatt atcatacctt cgtaaacttc tacaccaggg tctacaaaca
     1621 ttacagctct atcacttaag gcattaagag aataccccat agtaacacca ggtccttgtg
     1681 ctattaaaac accatttcct cttgaaggta tttctccttt aaattcttca aatttttcaa
     1741 atgaacgaac taaagtacct tctccacgag tagcgtttat aaattcactt cgatatccta
     1801 aaagaccacg agtaggtgct aagaactcta tttttacata gtctccttct attgacatag
     1861 actccatcat accttttctc atgtttaatt catttataat agtaccagag taaacttcag
     1921 gacagtttac aacaactctt tctattggtt ccattagttt tccatcttct ttatgcatta
     1981 aaacttcagg tttagaaaca ccaacttcat aaccttcacg tctcatattt tcaagtagta
     2041 tagataagtg aagctctcca cgtccagaaa ctttgtatcc atcagtagta tcaagaggtt
     2101 caactttaag accaacattt acttcaagtt ctttttcaag tctatctttc aaatgtctag
     2161 ttgtaacgaa ttttccactt tttccaacga atggagagtc gtttactaag aagttcatag
     2221 aaagagtagg ctcttctatt tttatcattt ccataggtag tggactatct aaatcgcata
     2281 tagtttcacc tatagatata tcaggaatac cagctataac aactatttct ccactagtag
     2341 cttcatcaac ttcaacttgt tttaaacctt catacacaga aagttttgat acttttcctt
     2401 tagatacaac agaatcttct ctacatatag caacttgtgt attgttttta agagtacctt
     2461 tgtaaattct acctatacca agtctaccta cataatcatc atatgcaaga gctgatattt
     2521 ggaactgtag aggttcatta tcgtaattag gataagcttc aacgtgattt acaatagttt
     2581 taaatagagg agaaaggtct tcactatcat catccatttc aagtttagca ataccttgtt
     2641 tagcaatacc atatattata ggaaattcgc attgctcatc tgttgcattt aagtctacaa
     2701 ataaatcgaa aacttcatta actacttctt ccgctctctg gtctttttta tcaatcttgt
     2761 ttataaataa tattggtttt aaaccgaact ctaaagattt ttgtaaaacg aatctagttt
     2821 gaggcattgg gccttcacta gcatcaacta atagaataac tgtgtcaaca gttttcataa
     2881 cacgttcaac ttcagaagaa aagtcactgt gtcctggagt atcaactatg tttattttgt
     2941 aatcttcata gtttattgca cagttttttg aatatatagt tatgcctctt tctttttcaa
     3001 ggtcgttact gtccataacg cagtctttaa ctacttcgtt ctttctaaaa acaccgcttt
     3061 gagaaaggaa agcatcaacc aatgttgatt ttccagcgtc aacgtgggct ataactgcga
     3121 tatttataat tttatgtttt tgtgacattt ttgttgctcc cttcgattaa aataacttat
     3181 taattatagc acaatatatt attaggtaca aggaggaata tattaaattg tattaatata
     3241 gtttaaattg gataaaactg ttagttttgc atctgaaaat tattctgttt aaaaaaattt
     3301 tttagacaaa taataacctt aatagataaa aagattaata aaagagtaaa ggatgatata
     3361 tgttggaagt aaccaaaata ataattaaat attttgcaag tgaaagtgat acttttgtaa
     3421 aagaagaagt ttgtaatttt gaagaacttc ttaagtggtc tcataaattt attgaaggta
     3481 aaaagataga agatgttttg tatataaagt ctaaagaaaa ctataaagta aatgtgatta
     3541 agtctttaga aattaaggaa ttggaaatag tgagaaaaca acagatacca ccgtacatgt
     3601 agtattaata atatataaaa ttaatatgta atactcgcaa taggatataa atggtatata
     3661 tggtaataat ttatattaaa aaaactttta taaagtttat atttgggtat aaaagtatta
     3721 attagacatt aattaggagg aacttaaata tgataagagt atgtccaaac tgttcaaatg
     3781 tggatgtaaa taagctaaaa actttggttg gagaagaaaa tgtaaagact ggttgtatag
     3841 gtcagtgtag agcatttaaa aaagaagctg ttggaataat agacaatgag cttgaaataa
     3901 aggaaacaga agcagagttt tttgaagcat gtaaagaata aattttaaat aaaatataaa
     3961 ttataataaa tagagcatct aaattgtata agcattaaac
//
LOCUS       DACULQ010000240         3995 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.242, whole genome shotgun sequence.
ACCESSION   DACULQ010000240 DACULQ010000000
VERSION     DACULQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.242"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            121..1524
                     /locus_tag="JA154_17275"
     CDS             121..1524
                     /locus_tag="JA154_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT7997580.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1615..1855
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1898..2596
                     /locus_tag="JA154_17280"
     CDS             1898..2596
                     /locus_tag="JA154_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAT7997581.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2619..3620
                     /locus_tag="JA154_17285"
     CDS             2619..3620
                     /locus_tag="JA154_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAT7997582.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1498 a          453 c          725 g         1319 t
ORIGIN      
        1 taaaactgtt tttgaatatg caaatatttt gaaaaatatc agtttaataa taatgaatct
       61 attttattaa ttttgaaatt aaatttaaaa taattaatag agaggggaaa ttaaattagg
      121 atggaaaaga aaaaaatagg cctatggagt ttggtattta tgaatgtatc agctttatat
      181 ggtataagat ggatagctaa atctacagca tcaagctttg gtttaggatt aggtgcaata
      241 cctatgtggt tattattctc atttatatac tttgtaccag gagctttaat ttgtgctgaa
      301 ttagcttcta catatcaaaa aggtgatgga ggtctttacg attgggttaa ggaagccttt
      361 ggagaaaagt atggattttt agtttcttgg ttaaactgga cagctaaaat attttggtat
      421 tcttcatttt taactttctt agcagtaaat gtttcttata caataggaaa tccagccctt
      481 gctgataata agatgtttgt acttatatta tctttaataa tattctgggc attatctctt
      541 atagcaacta aaggaataag ttttacaaaa atatttacta atacaggtgc attaggttca
      601 acaataccaa gtgctatact tataatattt tcatttgtag cagtatttgt attgaaaaaa
      661 catgatatag cttctacata cacaatagct tcaatgactc caaaacttaa tatggatgca
      721 tttgttgcta tatcagctat aatgtttggt ttagcaggag cagagactgc tgctaacttc
      781 ataacagaaa tagataagcc tgaaaagaac ttccctaagg caatattaat atcagctgga
      841 atagtagctt cattatatgt attaggttca attgcaataa caatgataat accaccagac
      901 caaattactg cttctaaagg tgtattagat gcactttctg ctgtttgtgc gtctttagga
      961 attggttctt ggttggttca attaatagct tttggtatag ctttttcagt attaggagca
     1021 atagttcttt acatagcatc accaataaaa atgttatttg gtagtgttaa aaaaggaata
     1081 tttccagata gtttagtaga ggtaaatgaa cacaagatac cttctaaagc agttatactt
     1141 caagcaataa tagttactat aatactattg gtgactacat taatgccttc tgttgatgct
     1201 atatacaatg ttcttgtaac tatgacagca ttaactgcat tattcccata tgtattgtta
     1261 tatgcttctt atattaaact aagaaaagag agaccagatg aaattagacc ttatactatg
     1321 gcaaaatcaa caagtacttg cctaggttta gcaaaaatgg ttttagttgt aactgtagtg
     1381 ggtatattat tatctgcagc accagttatg cctacatttg ctgaaaatgt tatatatgag
     1441 atagagatga ttggtggagg tcttctagtt atattatctg gtctatggtt atggaataga
     1501 tatgaaaaga aaacagctaa ataaaatgta ttttaaaaat ttcttttgga tattataata
     1561 tatagttgac aatttatagt gtaaggaata ttataaaata aagaaattaa taaattaaat
     1621 cctgagataa gaaatagtaa ataaatatat ttttgcacag agaattaagg atgctgagaa
     1681 cttaataaaa agtatttatt gaatgggtct ttgaggagta aaatgaaatg taatttaaga
     1741 ttacagagta tttgatatcg tgtatgcacg ttatagcata tattaagata tatatattca
     1801 attttgagtg tatataaatt taggtggtac cacggaaaat atatccgtcc tattgattta
     1861 ggacggattt tttaatatag aaaatttagg aggaaaaatg aacgtaatag gaattatagg
     1921 tgcaatggat gaagaagtta gtattttagt tgatttaatg gatattagag agactataaa
     1981 aaaagctagc ttagaatttt ataagggaat tttggaaggt aagaatgtag tgttagtaaa
     2041 gtgtggcata ggtaaggtta actctgctct ttgtgcccag atactaataa gcgaatttaa
     2101 ggttgatgct attgttaata caggtgttgc aggagcttta aacgaaaaac ttgatgttaa
     2161 tgatatagta atatcaactg atgctataca gtatgatgta gacactacag catttggaga
     2221 ccctaaggga gttataccaa gaatgaagac ttctgtattt aaagcagatg aaagacttat
     2281 agatgctgct tacaaatctt ctgtggaaga agtaaagact cataaagtat taaaaggaag
     2341 agtagttaca ggagataaat ttataaattc taaagaatta aaagaagaat tagtaaatga
     2401 ttttggtgga tattgtggag aaatggaagg tggagctata gctcatgtat gctatttaaa
     2461 taatactcca tttgtaataa taagagcaat gtctgataag gcagatggaa gtgctgatgt
     2521 tacttatgat gtatttgtac atgatgctgc caataactct aaagacatag ttttaaatat
     2581 gttaaaatca atataaatat ataaggaggt acaaaaaaat gagtgaaaaa aaagttatat
     2641 taagtggtgc acagccatca ggcaaaatga ctttaggaaa ttatttagga gctataaaaa
     2701 actggactga acttcaagac aattatgatt gttattatag tatagttgat ttgcatgcaa
     2761 taacagtacc tcaagaccct aaagttttaa gagccaatac tatagaatta cttgctcaat
     2821 atatagcttg tggattagac ccagaaaaaa atactatatt tatacaatca catgtcaaag
     2881 agcatgttga acttatgtgg gtattgaata ctatgactta tatgggtgaa ttaagtagaa
     2941 tgactcaatt caaagataaa tcacaaaaga gtgaagcaaa tctaaatgca ggtttattca
     3001 catatccagt tcttatggct gctgacatat tgttgtatca aactgaccta gttccagttg
     3061 gagatgacca aaaacaacat ttagaattag ctcgtgattt agcaaatagg tttaataata
     3121 gattttcacc tacttttgta gttccagaag gatactaccc aaaaggtgga gcaagagtga
     3181 tgagtttgca agagccaact aaaaaaatga gtaaatccga ttcaaatgaa aatgcattta
     3241 tactattagc agatgattca gattctataa agagaaagat aaaaagaagt gttactgatt
     3301 ctttaggtgt tgtaaaatac aatgatgaac aaccaggtat taaaaaccta attgatatat
     3361 attctaattt aagtaaaaag agtgtagaag agattgtaaa catgtatgaa ggaaagggtt
     3421 atggcatatt taaagaagat gtggctgaag tagtatcaga agctttaaga cctataagag
     3481 aaaaatatgt tgaccttctt aataataaag attatttaga aaaaatatat agtatgggag
     3541 cagaaaaagc cgaaaaacaa gctagaaaaa ctcttagaaa agtttataaa aaagttggat
     3601 ttatagagag aagatactaa ttattagcta tctttacttg ataaataaat tttaaagtta
     3661 ttaaaaaggt atatgatatg aaagagagag tctcaaagtg aacttttgtt catgaggcac
     3721 tcttttttgt atgaaataaa atatatttta atcatttcaa caatgaaaaa gaggattatc
     3781 tctattttga gacagcatct tttgataata aaaaaagatg agcttttgct catctcaaag
     3841 gggttagatt attcttactt tggggaaagt agaataaagg taaaagttaa ctgatgtcaa
     3901 ctttgtcttt tttgcatatc actactgagc ataaaaaaat cagaaaacca tagccttctg
     3961 aaacaacttc taacatacat tgtattttat accca
//
LOCUS       DACULQ010000241         3990 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.243, whole genome shotgun sequence.
ACCESSION   DACULQ010000241 DACULQ010000000
VERSION     DACULQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.243"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(50..913)
                     /locus_tag="JA154_17290"
     CDS             complement(50..913)
                     /locus_tag="JA154_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997583.1"
                     /translation="MMLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITG
                     LGVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMA
                     TLDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGI
                     VDCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISK
                     KIMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIH
                     EALGNGFKKIE"
     gene            1059..1949
                     /locus_tag="JA154_17295"
     CDS             1059..1949
                     /locus_tag="JA154_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT7997584.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAA"
     gene            2167..3921
                     /locus_tag="JA154_17300"
     CDS             2167..3921
                     /locus_tag="JA154_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HAT7997585.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         1625 a          421 c          626 g         1318 t
ORIGIN      
        1 aaaaagttga atttgtatat gttaattata cttgctattt atttgttagt tattctattt
       61 ttttaaatcc attacctaaa gcttcatgta tatctgtaat tataataaaa gcattagggt
      121 cgacattttt tactaatttt ttcaattcga caacttcttt cttttcaact actactaaaa
      181 gtacattttt attttctcca gtatatgcac cttctccttt tagaatagtt gcacctctat
      241 ctaaatcttc cattattttt ttacttatat cttttgaaag gtcagatata atagtgaatg
      301 atttggaata attaaatcct tcaataagca agtcactaac ttttactaaa atgtataaag
      361 ccacagttga ataaagtgca atttcaacct gtctatttac tatacctgat aaaacaacta
      421 caacgcagtc tacaataccc attaaaacag gtatactaaa tgtaggaaaa tatttattta
      481 gtaggagtgc cattaaatca gttccacctg tagagccatt tattctaaaa ataagtcctt
      541 gaccaactcc cattaaaata gaaccagata taattgcaag atacatatca tttgttatat
      601 ctaatgtagc catatttgca gtaagcttaa gtgctatggt aagaaaaatt attcctaaaa
      661 ccgttttaaa acaatctttt tttgaaagta ttttgtaagc aaatatgaat aatggaacat
      721 ttaaaagtaa attgacaatc caaagttcta caccgaataa tgaatttaaa acaactccaa
      781 gtcctgttat accaccagca gcgattgcat gtggattaaa aaataagtta agagaaatgg
      841 ccattaatat acagcctaca attaatatta aaatttctga aaaagttatt ctatcttttt
      901 tcaataacat cactccttat atatagatta taatatatat tatatagttt ttccaatttt
      961 taataaaaga aataaaaata tattataaaa acaatttgat tggaattttc agaaaattaa
     1021 tgctataatt aaaagtaaat ttaaagggag gttataaaat ggctaaatta actctagaac
     1081 atataaaagg aaatacatat tatattccac tccctacaat agtaggtgta tacgtagatg
     1141 gtaaggatgc aattttaatt gacagtggaa ataataagga tacagctaga caagttttaa
     1201 gattattaga agaacataat ttaaatccta aattaataat aaacactcat tcaaatgcag
     1261 accatatagg aggaaatgca tatttgaaga atcaaacaaa atgcaagatt gctacgacta
     1321 aaattgaagg atattttaca gaaaatccaa tattagaatc agcattttta tatggtggct
     1381 atccaagtaa agcactgaag aataaatttt tattagcaaa ggaaagtgaa gttgattata
     1441 ttataccttc aaatggcaaa attgtagata cagaacttga agcgatttct cttccaggtc
     1501 attattttga aatgattggt gttagaactc cagataatgt tttgtttgtt gcagatagtc
     1561 ttacaccaga gaatataatt actaaatatc attttttctt cctactggat atagattcac
     1621 agtttaagac actagaaaaa ttgagaatgt tagaggctga tttctttgtt ccaagtcact
     1681 ctgtcaaaac tacagatata aaaagtttaa ttaatattaa taagaagaag atggaagaaa
     1741 taatagataa tattaaaaag gtttgttatg agccagttat gattgatgaa gttattgaaa
     1801 aaatgtgtga tttgtacaat gtcaagcttg atgctaatca atatgtgttg gttggaagta
     1861 caatacgttc ttatataact tatttatatg agaataatat ggttgaatat atatttgatg
     1921 gtggtaagat gatgataaaa gctgcgtaaa tagtaataat tttcaaatta tatgatgaaa
     1981 ttgttttagt atgactgtta tttgaaaagt aatataaaaa aatttatata aataagctaa
     2041 gatgattgat ataaagttac tgataatttt aaggtaatat atacgttact ataatattat
     2101 gttattattt gtaaataagg ggataattta taagcaaatt aaagacctta atttacagga
     2161 ggaatagtgt atattatgaa taaaaaattc aaaaaattca gtaaaaaact aagtatatta
     2221 attttagttt tgactatgtt tctttttaat aatttaactg caaatgcaca gattaaagaa
     2281 gattcaataa taggaattga tagatatgaa acagcaggac ttatagcgga tagacaaact
     2341 tatgatactg taatattagt taatggagat aaaaatttat ctgatgggct ttcatcaagt
     2401 ggattagctg gagctattaa cgcaccaata cttttgacta aaaaaagtga aataccaaaa
     2461 gctactttaa atagactaga taataagagt ttaaatgttg taaaaaaagt atatataatt
     2521 ggtggttata atactattga aaattcagta gaaaaagata taaaaagaaa aggtattcaa
     2581 gtggaaagaa taaatggtaa caatagaatt gaaactagtt ataatgtagc taaaaaaata
     2641 aatgaagtaa gtagtgtaaa agaggtaatg cttacaaatg gatttgtagg agaggctgat
     2701 gctatgagta tagcacctgt agttgcaaaa aataaaggag ctataatact tacagatggg
     2761 aaatcagttc catttaagac tgaaggttta aatgtttacg ctataggtgg caaatctgcg
     2821 ataaatgagg atttagtaaa tgaaactaaa gcaattagaa tcggaggaaa tgatagattt
     2881 gagaccaata aaaaggtaat ggaaaagttt tataatggag ctacagattt ttatataaca
     2941 aatggatatc aattagtaga tgcactaaca ttatctcctt tagccaaaga aaaaccaatc
     3001 gttttagttg ctgatggaag taataaaggt attttaaaag gagctaaaag tataacaaaa
     3061 gttggaggta ttgatgccaa tatatataaa cagtgtttag atatgattaa atataataat
     3121 atggatatga ctccaaatat tttaaaacac ttaacttcag ttgaaggaaa tgaagtttct
     3181 gaaatactta tagaatcaga taatttaggt gtggttgttg aaaagacgaa taccaataaa
     3241 tttgagtttg attacgtaag tgtaactaat gaaaaaaatt gtactttttc tgtatataaa
     3301 gaaaattctt ctaataatat aaaaaatgga aagctagttg taagtgctaa gaaaaaaata
     3361 gataagaaag atagtgattt gcaagatatg aatggcgaca atatgataaa tgcaaataaa
     3421 gataggatgg taaatgtgat aaaaattgga gttcccgata aaatgtattc taattttaaa
     3481 gtagatgttg aaagtggttc agtagaactt tataatatta aaggaggagc tactgttgat
     3541 gtcaatgacg gaattgctaa cattgttgat aacaatgtaa caaatccttt caatataaat
     3601 actaatagtg ggatttcagg tgtaacagct gaaactatat catctgaaat aaaatttaga
     3661 gcaaacaatg gaattgtaaa tgtaacagct acaaatataa atggcaatat aagtttattt
     3721 ggaagcgata ataaagatac ttttgatgga atatttaaat taaatctgaa aaaagaacca
     3781 tcaaatttac acttgaagtt aattggaaat gggataaata aattgccaga aggttggtct
     3841 aaagattata ttttaggaaa tggacaacca attattgaag ttaaaaataa tggcataaat
     3901 aatatatcaa ttggagaata aaaacatatg aaaataaagt ttatatagtt ttaaaaatta
     3961 tagaatttta aaatatttat atatattctt
//
LOCUS       DACULQ010000242         3983 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.244, whole genome shotgun sequence.
ACCESSION   DACULQ010000242 DACULQ010000000
VERSION     DACULQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.244"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(489..899)
                     /locus_tag="JA154_17305"
     CDS             complement(489..899)
                     /locus_tag="JA154_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997586.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1115..2497)
                     /locus_tag="JA154_17310"
     CDS             complement(1115..2497)
                     /locus_tag="JA154_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAT7997587.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNGKDVLYTDSGEVSIEQSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2508..2867)
                     /locus_tag="JA154_17315"
     CDS             complement(2508..2867)
                     /locus_tag="JA154_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT7997588.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3072..3851)
                     /locus_tag="JA154_17320"
     CDS             complement(3072..3851)
                     /locus_tag="JA154_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-carotene 15,15'-monooxygenase"
                     /protein_id="HAT7997589.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTVISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         1402 a          659 c          382 g         1540 t
ORIGIN      
        1 tttaaacaaa tcttaaaaat cctaaaatgc taattttctt taacttttaa tgaatctaat
       61 agataacatt taattttcgt tatatttttc agaatatttc tattattatt tttaagaaaa
      121 aaaaataatg ttaacatttc ttaacataca ttaaaaaatg ttaacattat aaattgggtt
      181 taatactttt actttgactc gatttacttt tattctactt cttactttat ttttaactta
      241 gactttattt ttttattact tatttgagtc ttatttattt tattatcact ccatatgttc
      301 aattacattt gtctaaattc tttgatactt ttattacata attttaacaa gcttaagtgt
      361 tatttgggta gtgcagttga taattattta aataatcatc aactgcatgt ttatatcatt
      421 acagtagctt aatgtattta atcatactta cttaggattt tgaaatatct tagagattat
      481 atttaacatt agtcaattgc tgcataattg taagcaattg aaatttgaga cccatcttca
      541 ttttctatag tgataggctt cataattttt aatccttcag tttttatctt ttctaattct
      601 tgttccattt tcttaattgt ttcctcaagg tctttttgac ttacatcttt gactgcatta
      661 gggtctttag catagtcttt taattcttga atatgtttag catactcttc ataagtataa
      721 tgctcaacac catacttttc caattcagta gggtcagtta ttcctgattt tattagagat
      781 tcaagtttag gtaaagcaat attagatgaa tcatttccat ttgcaaatac tgctccagtt
      841 cctcctagta ccattgcaga taccatcatt gcacttataa gggatttttt tattttcata
      901 accaatcttc ctttcttaga gatttatttg ggtagtgtag tttgaaatga ctattgcaat
      961 agttatcaac cacatcctta gactatcaca gtagtcatgt ttatgtcaag cctttttaaa
     1021 aaacttttca ataaaaaata ctatctcaaa acaaaattaa ttattttgag atagtatttt
     1081 aattctaaat cttttatatt actaagattt atggttactc tatccatgtt ctatattgtt
     1141 ctttattaac tgctaacaaa ttcccttttc ttgatatgac aaagcgatta tcattatagc
     1201 cactttgctc aattgaaact tctcctgaat cagtataaag tacatcctta ccattactag
     1261 atttatttac atacgtttct tcgctattct cagaattagt tactttgctc atagatgatt
     1321 cttcatgctc tctaaattca attacagata cttttgccgt actaaaatga gtctctccat
     1381 cacttttagc tttactaaca agaatatcag attctacttt gggagaatat aaatcagttg
     1441 atttactact tattgaaaat ccaattttat cactttgttc attttttgtt aaaccaaaag
     1501 attcaacctc attaatctct ttattttctg ttgcaaaaac aagacctgtc ccacaaacta
     1561 atacagttga aactaaaaaa gtaacataag ttgatttttt aaatttcata atattaacta
     1621 ttctcttttc tatttttgtc tttccaaatc cactacatat tgtcattgaa aatggattat
     1681 tctcctctgc tagacttatt agtgtggttg cataatcagc cctatattct tcaccaagta
     1741 tactcataac cttttcatca caactaaatt ctatgtcttt atttagtaaa aaaaacatca
     1801 cccaaactaa gggattaaac caatggacaa caagagctac aagcataaat aactttaaca
     1861 gattatcaaa gctattaata tgaaccattt catgagttaa aacaaagtct aaagtttgat
     1921 tattagcata attcatttgc tttggcaaaa taattcgtgg ttttaaaata ccagatgtaa
     1981 gtggtgtcct agtaaattca gatatgcaaa caataaatct tctaaaacgg ctattcttac
     2041 acatccattt tttaatgaac tcatgatttt ttatatgaat tgaagtttta aaaagattaa
     2101 ttattttaaa atatctaaag cataaaagta tgctaaacat aatagtaact ataaaagaca
     2161 aaatgcccaa catttccaac atcataatat tttcaatatt agacattatt tcaaattcgt
     2221 cagaattgtt tttacctgta gagtcactag ctttcttaag agtataattt tctgttgatg
     2281 tattacccaa ttccacatca aaagtcgatt caattatact ataaatacta aaaccagaat
     2341 ttaaacttac tggtattaga agtttaaata aaacaatcaa ccacaataat acaaatacct
     2401 ttttaggtaa ttttttaaat aaacatgctc ttataaaaat aactattaaa agtaatactc
     2461 cagaagtaaa actcatttct aacaatttaa tgaacataaa tcttacctca ctttaatttt
     2521 tcaacaattt gtttaagttt tgtaatctca tcacttgaaa gattttcttt attaataaaa
     2581 gctgaaagaa atttttctga agaccctcca aacattttat taattaaagt ttctgtttcc
     2641 tgtttttgta cttcctcttt gcttattaaa gctttacaaa caaaattcgg ctcaattctc
     2701 tctattgcac cttttgacac taattttttt ataacggtat acgtagtgtt tctattccat
     2761 cctgtttctt cttttaaaat ttttactaac tctccagcct tgctttctcc tctctcccat
     2821 agaacttcca ttactttaag ttctgaatca aataatttta tctccatata aaatcgctcc
     2881 tcaaatatag ttatcacaat agtcattact ataataaaaa actatcatag tagtctatac
     2941 tatgtcaatc atttattttt taataataaa aactgtcttt ttaaaaaatt cttataaaag
     3001 acagttttat caatgttata ctttaatctt taattaaagc catattttat ttaaatatat
     3061 aactatatat cttagtacat agtataagat ttttctttat ctaatagtat atcctctaca
     3121 tctgggtttt cttttactaa ttgtctataa aaattacaat tagtaacacc tacatatgga
     3181 attagccata gtagacctat tccaagagta gctattgaaa gtatatacca tcctataaaa
     3241 ctaaagttta gtacaaataa tttaagtaca tttcctttca tcatatctaa actagcaccc
     3301 attgatttaa atacacccat attatccttg tcttcacaaa ttaagaaagt agcttgagaa
     3361 acatataata gcaatatata aaccggtaac ataagaacaa gtattataag aactagagtt
     3421 attccagtag aaataacagt ccctattcca acattatcac ttgataaata ttcaattgct
     3481 ccactagtaa gtaaaactcc aaatgataaa aacgctccta taattgtgat tataaaagaa
     3541 taaatataaa caataattga ttcaaataaa taaaaaccaa aacttcttaa ataaattctc
     3601 ccacttacaa agatatctcc tatcttgact ttttcatctt tagtaatttt caagaacata
     3661 catgtaaatc ctgctgaaac gcaaaaagct attacagcca ctacaatagt agtcaatggt
     3721 tcaaaaggta atcctacaat tgatattaat actatcaatg caatatatac tgccatagaa
     3781 attgcacata tcccccagtt tccagataac tgagctttag atactcgttt taattcttgc
     3841 cttgatacca taaaaaacac ccctttcatt ttataaaaaa acttttaaaa atataaataa
     3901 tcttttaacg tataatatta atatatcata ttttcgacta tataagatac aattattcac
     3961 catgttttat caagatatac cat
//
LOCUS       DACULQ010000243         3889 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.245, whole genome shotgun sequence.
ACCESSION   DACULQ010000243 DACULQ010000000
VERSION     DACULQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.245"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(147..347)
                     /locus_tag="JA154_17325"
     CDS             complement(147..347)
                     /locus_tag="JA154_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAT7997590.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(572..748)
                     /locus_tag="JA154_17330"
     CDS             complement(572..748)
                     /locus_tag="JA154_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997591.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(802..891)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1071..1271)
                     /locus_tag="JA154_17335"
     CDS             complement(1071..1271)
                     /locus_tag="JA154_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997592.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1273..1623)
                     /locus_tag="JA154_17340"
     CDS             complement(1273..1623)
                     /locus_tag="JA154_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997593.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     SKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1785..1974
                     /locus_tag="JA154_17345"
                     /pseudo
     CDS             1785..1974
                     /locus_tag="JA154_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2586..2690)
                     /locus_tag="JA154_17350"
                     /pseudo
     CDS             complement(2586..2690)
                     /locus_tag="JA154_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417378.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
     gene            complement(3010..3504)
                     /locus_tag="JA154_17355"
     CDS             complement(3010..3504)
                     /locus_tag="JA154_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HAT7997594.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKSDYGSSIDIMNEQNKIDIVIKFLND
                     KSNT"
BASE COUNT         1503 a          514 c          420 g         1452 t
ORIGIN      
        1 tatttttgtc agatttattt tataaaaagt atttagacta agtattattt ttaagtattt
       61 ttttgttgaa gttatttttt ccttttttac aaaatgcgta aataaaaaaa agactcttaa
      121 aagagtcttt ccgaaaaatc tatatattat aatatagtta tattttctgc ttgaggacct
      181 ctagcgcctt taactatatc aaagcttact ttttgacctt cttctaatga tttaaatcct
      241 gaagtttgta tagctgagaa atgagcaaat acgtcatctc cactttccat tgatataaat
      301 ccaaatcctt tttcattatt aaaccatttt actattccat tattcattaa aaatactcct
      361 attaacctat atcaagatat acttgattta cttgtgtatt accacaaata ctattgtatc
      421 atagattaac ataaatgtct atttaattat aatttttagt taacattttt atttattctt
      481 aatatgaaaa taaatttaaa cattttatat taccaatata ttaatgtaaa tgtagataaa
      541 actaaaaagg gttttctaga aaaaaatctg tctaattata cttacttacc aataccatca
      601 cttgatgcaa gcaagattcc acaacttttt ctccgtactc taatataaaa tcatctaact
      661 gtgtatctat aaaatcacaa taatcatatg aactaccaat ataagcagtt aacatatgtg
      721 tacatttatc cattttatct ctaaacataa tgtatttatc cccctaaaaa actttcatga
      781 aaaacaaaaa gaaaatatct ttttggcaac tggctgacat aacttatttt ataagcgtta
      841 gtccctatag ctttgcgtca ctaaatttct ctagttttgc cgatttagtt ttattctaca
      901 gattaataat acaatagaat tagttattat tcaacatatc tagctgaaaa attaataaat
      961 ttatatatga acgttaaatt atctgaaaaa ataaaaagcc cctctgtcag gagtgactta
     1021 aaaattgaat ttaagtttgt atttaaaaca catttttaat aatgcaaaaa ctaatcaaaa
     1081 ataaacaatt catcaatagg tgtatctact atttttgata tatctattgc taatttcaaa
     1141 gaaggattat actttcctgc ttctaaacgc ataatggttt ctcttctaac tcctactttt
     1201 tctgctaatt gttcttgagt taattctttt aattgtctat atttttttag gttacaagtg
     1261 aatttaggca tattattttt cctctaaaat ataaagtttt atagcataaa catttaaagt
     1321 gattgcatat gtaattgata gtaaaacaca cattaattct atattcctag acactatagt
     1381 aatacaccat aaaatgaata attctataat agcaagctgt cctgtaaatg ccatagctgc
     1441 tttttcatct tgtacatacc tttcatctgc tttatcacca tttatttttg atataataaa
     1501 gtttgagaaa aaagcaaaaa atccaatata tgtaaggtca gcaacattgc cagatgaaaa
     1561 atatctaaga cttaaaaatc caaggaaacc acaaaaaccc ataaaaaatt ttttctgaat
     1621 cattttttat cctccttagt aattttaaaa gagatatatt tatatcttta tgatacaaat
     1681 atatctcttt tatattcttt tgtcaataga aaatcagtat tatacaatat ttgtactgca
     1741 ccataattgt tagagcaaaa atctactgct tgagatgttt atgtatgact aaatatgatt
     1801 ttaatttcaa attaaaagta gctaaatttt atttgaatag agaaggaaga gatattttaa
     1861 tagtaaaaca atatggagtt tataaccatt ctcaagttga aagatgggtt aatatattag
     1921 gagagtgtgc actaaaaata aaaaaaattt tctatactat atggttcaag ttagatgtta
     1981 taaactatat gatgataact aaatatagtg tacaataaat cgctaagtat tttggaataa
     2041 acaatagcac agttatagca acatgaaaat aaaaattttt acatagtggt gttgatgcct
     2101 tttctaaaat gaaaggaaga ccataatgaa taaaaacaaa aacactaaag aaaaaataaa
     2161 ttaacaagag aacaagaatt agaatatgaa aataaatttt taaaagatga gcttgagttt
     2221 ttaaaaaaga tgtgtgcctc agggaagcca cattaaaaaa cagtggtatc attattaatc
     2281 aaaagaaagt tagatgtatt atcaaaaaat tagaacttaa atgcatagca ttttttcata
     2341 aatcacataa atataactcc tataaatgta taattggaaa agtaaaaaat aataaaacaa
     2401 atagaagatt taaaatatca atagcatatc agaaaataac aaatgatatg ctagaattta
     2461 taaaatatat ctaatgataa aaatggaaaa tcaattataa aaaaggcata tcccgattca
     2521 ttttttgtta tatagtcaaa taagaatact atagatgaaa gatacgattt tgcatatgta
     2581 ttaagtaagt attctgatgt ttttggttga tacacatgca ataaagaagt tgtgtttgca
     2641 gataaattag caaaactata gtataagttg caacaataaa gaaaatgaaa attatcatac
     2701 taaagcataa ggcatgttac aattttatta gataggacat taacatacat taaagtactt
     2761 tttattataa agagtactta tttttttgaa attcattaat gtataaacta tcaagaatat
     2821 tactcaacat aatttaaaaa caacttgaat aggtattttt aaattatgtg atataataaa
     2881 aaatagaagt gtatgccaat acacttctgt aattataact aagcattcta ttcttgagga
     2941 gtgctttttt catttctcca aacaagattg tttatttcat cagcaattat taaataaaat
     3001 ttattgtatt taagtattcg atttatcatt taaaaatttt attacaatat ctattttgtt
     3061 ttgctcgttc attatgtcta ttgaacttcc ataatcactt tttaatttat ctagaccact
     3121 tttgcttatt tctccttctg gaagtatact taattctaga tatttttcat gaccaggaga
     3181 tgacattaat agatatgaac cattataagg catacctaaa ttttctattt tatctgctaa
     3241 tgggtcatct ttaaagccac catttattat tatttttcca tctttcttta cccatgatat
     3301 cattcctcca tcagtatatt ttgattcatc tgcatagtag ctaagagacg cactttttat
     3361 tgcacttata tcagcttcaa gtttagctat ctttgctttt tctatatttt taaataatgc
     3421 tggaagtgct actattgcca atattcctat tatagcaatt actactaata attccactag
     3481 agtaaatcct tttttatttt tcatttattt cccctccata taaaattaat aatatataaa
     3541 tttttatttt gttgatttct actttctata ttttaccata tatagacaaa aaaatcactt
     3601 tctatgacaa atatactatt attttacaat ttatggaaat gatttaaaat gacatttctt
     3661 gttagaaaag atataagaaa tggattaaaa taaagaaaac tacagtcata tcaatgtcaa
     3721 taatattatt taaaagcaga tgaaaataaa aagaggactc agaaaataat gtctatttaa
     3781 ttatacttac ttaccaatac cataacttga tataaacaag attatactac atttttttta
     3841 tgctctagta taaaatcatc taactgtgta tctataaaat caaaataat
//
LOCUS       DACULQ010000244         3882 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.246, whole genome shotgun sequence.
ACCESSION   DACULQ010000244 DACULQ010000000
VERSION     DACULQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.246"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..382
                     /locus_tag="JA154_17360"
     CDS             <1..382
                     /locus_tag="JA154_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAT7997595.1"
                     /translation="HVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNIKINLNIK
                     GYKFLPYVKNLQASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFGNMKSIY
                     LKKTNGNLIELSNHRITESKPWKI"
     gene            370..1308
                     /locus_tag="JA154_17365"
     CDS             370..1308
                     /locus_tag="JA154_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAT7997596.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFRLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1692..1916)
                     /locus_tag="JA154_17370"
     CDS             complement(1692..1916)
                     /locus_tag="JA154_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAT7997597.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2509..3801)
                     /gene="eno"
                     /locus_tag="JA154_17375"
     CDS             complement(2509..3801)
                     /gene="eno"
                     /locus_tag="JA154_17375"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089687.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAT7997598.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1318 a          638 c          441 g         1485 t
ORIGIN      
        1 acatgtggtc attactaata ataatataga aaattatatc aatttttata aaaaatttgg
       61 tttttcatct aaagaaatga gcaaaaatta taaacttaat gctagaaata ttaagataaa
      121 tcttaacata aaaggttata aatttttacc ttatgttaaa aatttacaag caagtagaga
      181 taacttatgt tttaaaataa acacaaatat tgatgagcca aaaaaatact tagaacaaaa
      241 tgagattcaa attaagcgag gaattgtaga atgtacaggt gtctttggta atatgaaatc
      301 tatttatttg aaaaaaacta atggaaattt aattgaatta tcaaatcata gaatcacgga
      361 gagtaaacct tggaaaattt aatcctatca ataaatgttg ttcttccttt gtttctcaca
      421 atgtcacttg gatatgttct aaaaaaatta ggtctatttg atactcatac tttaaataaa
      481 atgaataatg tctgcttcaa gtctttcttg ccattattat tattttttaa tatatacaat
      541 acagatttag atggttctct taattttagg cttatggctg tagctgttat aattattttt
      601 tcaatcttct taatcttagt ctttattatt cctaaaattg aaaaagataa taagacaagg
      661 ggtgcattaa tacaaggtgt atttcgtagc aatttcgtta tatttggtat tcctgtgaca
      721 atttcactat ttggcgataa gagtattgga gtcacatctt tattaattgc agttattgta
      781 cctatgttta atgtattatc tgttgtaatt cttgaaattt ttagaggtgg caaagtaaat
      841 ataaaacata tgattaaaag cgtcttaaca aatccactca taatcgcctc ttttatagga
      901 ttgacaatgc taattttaaa tattaatata cctacatttt tacacaaaac tattggtgat
      961 atttctaaaa tagctactcc tttatcactt ataattctag gtggctcttt tagtttttct
     1021 gctataaagg ggcatataaa ccccattata ttgggcgtat gtggaaaact tatacttgta
     1081 cctgctatat ttttaccaat tagcattttt cttggattta gaaatgtaga attagcttca
     1141 cttatgataa tgttatctgc tccaactgca gtttcttcat ttacaatggc acaacagatg
     1201 gattctgaca gtgaattagc tggacaaatt gtagtattta cttctggatt ttctataatt
     1261 acagtatttt taataatatt tattttaaag cagaataatc ttatttaatg atatttgata
     1321 acacaaattg gtattatgag aaacatatat aaaatcgatt taattgcgta aaaacatatt
     1381 attatactat ttttactata taatataact ttgtgtatat tatctcatta aacatggtga
     1441 aagtagtgtt gctatatatt ttttagtttg aaaaaaatat ataaatacaa acaatatata
     1501 aatacaaata ataaacgcca acaaatagtc aatctataaa ataaaaactg gactaatcat
     1561 ataaaatcat tagtccagct tttattttac gtacttttta tgttgatgta cttttattaa
     1621 aaactaaatt taatttttaa atgtctagca aatgcactaa ttaaataatg gccagttatt
     1681 caacaatatc actacttctt aactataagt aatgccaatg catttataaa aaataatact
     1741 gctgttatct ttgtaactct agcaagaaat gcttgttttc cttttggttt aaagtaagct
     1801 tggcttcctt ctccaccaaa ttctgaaggt aaagcattct tagaatcttg aggcattatg
     1861 ctaattacta gtaaaatccc tagtactact tgcacaccca ttaaaatgtt gctcattctt
     1921 gcccctccta tctgtttatt acttaaattt cattactctt tagtgtatca caataatata
     1981 gccatttcaa gactattatg ctataaataa catacaaaaa aatatattgt tactataata
     2041 tattactata ttctcaatat aaaaatgaat aaaacttaga agagaaaact atttaaatta
     2101 atgcataaat taacacttaa aataatctac tctcattgta tatattataa atagccacca
     2161 aaataaaaca atcattttaa aatattgata taacttttaa tttcaacata atttaaaata
     2221 aataatccac atttagcaat aatactttaa tgacaattat gattaaatca aaatatgtct
     2281 tcacttttat tttttaataa aaacctccta tgcaattaga taaaaatgag atagaaacat
     2341 ttattcatat tttttctgaa tctatgaata aaattaagta ataaaatata aaaaaggata
     2401 agtgaattga aaaatcaatt aacacttatc tcttttatat cttataagat gagcttaaat
     2461 atcttatatt ctctcttcat taatcttttt tagtaaccat tttaataact atttttttaa
     2521 gttatagaaa gattttaatc cacaatatct agcttgctct ccaaccattt cttctattct
     2581 taataattgg ttgtacttag caactctatc agttcttgat ggagcaccag tttttatttg
     2641 accagcattt actgcaacag ctaaatctgc tatagtagaa tcttcagttt ctcctgatct
     2701 gtgagatata acagcagtat atcctgctct cttagccatt tctatagcat ctaaagtttc
     2761 agttattgta cctatttgat taactttaac taatatagag ttagctactc cattttcaat
     2821 tcctttttct aatctctctg tgttagtaac aaataagtcg tcaccaacta attgtaattt
     2881 atttcctaat ttttcagtta ataatttcca tccatcccaa tcttcttcgt ctaaaccatc
     2941 ttctatagtt attattggga agttgtttga ccaatcttca tataaagcaa ccatttcagc
     3001 agcagttaac tcttttcctt ctcctgctaa aacgtatttt ttagtttctt tgttgtacat
     3061 ttctgtagca gcaacgtcaa gacctaacat aacgtcttca cctggcttat atccagcttt
     3121 tgttatagct tcaactatta attctaaagc ttctctgttt gaacctaagt ttggagcaaa
     3181 tccaccttcg tcacctacac cacaagctaa tcctttttct cctaaaactt tctttaacga
     3241 atggaatact tctgctccca ttcttaaacc ttctttgaaa caacaagctc ctactggtaa
     3301 tatcataaat tcttgaacat caacgttgtt atcagcatgc tctccaccgt ttaatatgtt
     3361 catcattgga acaggtaatt gtttagcatt tactccacct aggtattgga ataatggtaa
     3421 acctatttca tcagcagctg ctctagctac tgccattgaa acacctaata tagcattagc
     3481 tcctaattta cctttgtttg gagttccatc taattctatc atgattgcat ctatagctgg
     3541 ttggtcaaat gcatccattc cttctatttc aggtgctatt atttcattta cgttagcaac
     3601 agctgtttct acaccttttc ctaagtatct tcccttatct ccatctctta attcaacagc
     3661 ttcaaaagct cctgtagatg ctcctgatgg aactattgct cttcccattg ctccatcttc
     3721 taatactact tcaacttcta cagttggatt tcctcttgag tctaatactt ctctagcata
     3781 tactaattca ataactgaca tttgtatatc tcccttcgat tttctattag ttaaattttc
     3841 atttactgta aacatccgtc tactatttta atctactttc ta
//
LOCUS       DACULQ010000245         3853 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.247, whole genome shotgun sequence.
ACCESSION   DACULQ010000245 DACULQ010000000
VERSION     DACULQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.247"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            23..1498
                     /locus_tag="JA154_17380"
     CDS             23..1498
                     /locus_tag="JA154_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAT7997599.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1585..1998
                     /locus_tag="JA154_17385"
     CDS             1585..1998
                     /locus_tag="JA154_17385"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087363.1"
                     /note="Decarboxylation of S-adenosylmethionine provides
                     the aminopropyl moiety required for spermidine
                     biosynthesis from putrescine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase proenzyme"
                     /protein_id="HAT7997600.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2028..2879
                     /locus_tag="JA154_17390"
     CDS             2028..2879
                     /locus_tag="JA154_17390"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087364.1"
                     /note="catalyzes the formation of spermidine from
                     putrescine and S-adenosylmethioninamine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HAT7997601.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            2869..3747
                     /locus_tag="JA154_17395"
     CDS             2869..3747
                     /locus_tag="JA154_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAT7997602.1"
                     /translation="MKNNFYHMNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
BASE COUNT         1503 a          422 c          709 g         1219 t
ORIGIN      
        1 gagaggagat gaatttgaaa tcatggatag cgagtttgca aatgcagttg aattaagcta
       61 tgagtgtgct ccattattag agtcgttaaa ggaatactca gagaaagata tagcttgttt
      121 tgatgtgcct ggacatgtaa aaaatagagg tgtagctata ttaaataaat atctaggtga
      181 aagtctcatg aaaatggata taaattcatc accaactatg gataatgtat ctgcgccaaa
      241 tgggattata aaaaatgcac aagatttgtt agcacaagca tatatggctg atgaggcatt
      301 ctttataacg aatggcacaa ctcaagcaat acatgcaatg attttgagtg ttataaaacc
      361 aggtgaaaaa gtattacttc caagaaacat acataaatca gtaataaatg cgttaatact
      421 ttgtggagga ataccaatat ttatacaacc agaatttgat gaaaaactag gtataagttt
      481 aaatattact cttgaaaaag ttagaacaga gatagaaaaa gattgtaata taaaagcatt
      541 atttttcctt aatccaactt actatggagt ttgtgcagac cttgagagta ttatagaact
      601 atgccataaa aataatgttt tagtgttagt tgatgaagct catggtgcac attttccatt
      661 tcatttagat ttacctcctt ctgcaataag tttgggagct gacatggtag ctgtaagtat
      721 acataaaact ggaggagcat tgacacaatc ttcagcactc ttattgaata gagataatgt
      781 aagttttgaa aaggttcttc aatcaatcaa tatgctacaa tcaacatcag catcttattt
      841 gcttatggca agtatagatg gtgctagagt taatctagtt gagaatggtg aaaagcaatt
      901 gtcaaaagca ttaaatctat ctaggtatgc aaaatctaaa ttgaacaaaa ttgatggaat
      961 aaaagtttta tctacagaaa tattgaaaca aaaaggtgtt aaatttatag atgaaacaaa
     1021 attgtgtata aatgtaaaag aattaaattt gactggattt gaagtatacg atttattata
     1081 taaaaatttt tcaattcaag tagaattggg agattcatat aatatactgg cacttgtgtc
     1141 cattggaaca aataaatcag atatagatag attagtaaag gcactttcta taattgcaaa
     1201 gatttataga aaagaatcaa cattaaatga atttaatatg gttcaaataa atccaattat
     1261 taaacttaat ccaagagaag ctttttatgc accaaaggaa agtgtagaaa tcaattcatg
     1321 tatagataga atttgtggag aatcaataat ggcatatcca ccaggtatac cgattattgc
     1381 tccaggagaa ttgataacag aagaaattat ggagtatatt attttcttaa aaaatagcaa
     1441 tgcatatctt acagatgtac aagataaaaa cttagataga atactagtaa taaaataaat
     1501 tactttattt tgcaagggca agtgtttgtt aagtagagtt tatcaaatag aacttattaa
     1561 aagtataaag gagttggggt tgaaatgaaa tttgaaacac taggaagaca tatactagta
     1621 gaatactata attgtgatga aaacgtactt aaagacccac gtcttataga aaattttatg
     1681 aatgattcag cattaaatgc aaaagcgaca attgtggatt ctgtattcca ccattttaat
     1741 ccttgggggg tttcaggagc tgttataatt gctgaatctc atttgacaat acatacatgg
     1801 cctgaatatg gttatgcagc agcagatttt tttacttgtg gtgatataga cccatggaaa
     1861 agttttgaat tattagagca acttttaaag tctgaaagaa gcgagtctac agaaattcca
     1921 agaggcttaa ctacaaaaat taaaaaatat tcaaaaaaag atttaggtaa gattactcat
     1981 aaaccagaag cagtataaaa catacaagag aaagtgagga ttattatatg gaactttggt
     2041 atacagaaga atggacagaa aatgtaagat tttctataaa agtaaataaa catctatttg
     2101 aaggaaagtc tcaatttcaa aggatagatg tgtttgatag tgatgaattt ggaaaatttc
     2161 taacaataga tggattaatg atggtaacat ataaagatga atttatatac catgagatga
     2221 taactcatgt tcctatggct acaaatttaa acataaagaa agtattagtc ataggtggag
     2281 gtgatggagg tacagtaaga gaattaagcc gttatcctca aatagaaaag attgacatgg
     2341 ttgaaataga taaaatggtt gtagatgtat ctaaagagta tatggatata tgctcatgta
     2401 aattagatga taaaagagtg agtttatatt ttgaagatgg tgttaatttt gttaagtgtg
     2461 cacatgataa atcatatgac ctgataatag tagattcaac tgacccaata ggaccaggtg
     2521 aagggctttt ctcaactgat ttttataagg attgttatag aatactgact gatgatggaa
     2581 ttttagtaaa tcaaagtgaa agtccgtatt ttgactttaa tgctaaagag atgaaaagag
     2641 ctaataaaaa gttaaaacag atatttccaa tatcagaagt ttatcaagca catataccaa
     2701 catatccatc tggtcactgg ttgtttggat ttgcttcaaa aaaacttaat ccagtaaaaa
     2761 atcaagatag aaatggatgg gagaaattaa gtttaaaaac taaatattat aatagtgata
     2821 tacatttagg ttcgtttatg ttacctcaat acgtgaagga gatgctggat gaagaataat
     2881 ttctatcata tgaacacttt tatgagtatg aacaaaaatt atgaagaatc taatcttata
     2941 gtatttggtg ttggatttga tggaactact tctaacagac ctggggctag atttgcaagt
     3001 agctctatga gaaaagaatt ttacggtctt gagacatata gtcctttttt agatttggat
     3061 ttagaggatt ataatatatg tgattatgga gatttagaaa ttagtgttgg aagtacagaa
     3121 caagtcttaa aagaaatcta tcaagagaca tataagattg ttagagattc aaaggtaccc
     3181 tttatgattg gaggagagca tttagttaca ttaccagcct ttaaagcagt acatgaaaag
     3241 tataatgata tatatgtaat tcattttgat gcccatactg atttgaggga agaatataat
     3301 aatagtaaaa attctcatgc aacagtaatt aaaagaatat gggatattgt aggtgataat
     3361 aaaatatttc aatttggtat aaggtctggg acaaaagaag aatttaaatt tgctacagaa
     3421 gaaaaacaca catacatgga aataggagga atagatacgt ttgaaaatat agttaatatg
     3481 ctaaatggaa agaatattta tctaactata gatttagatg tattggatgc atctgtgttt
     3541 ccaggaacag gtacaccaga acctggtggt gtaaattata gagagtttca agagattttt
     3601 aagattataa aaaactctaa tataaatata gttggttgtg acattgtaga gttaagccct
     3661 gattacgata caacaggtgt atcgacagtt atagcttgta aaatcttaag agagttatgc
     3721 ttaataatat ctgataaaat taaatagagt attttatata aatagtatat attataatat
     3781 aagaagttat tttaagtttg acgcttttct aaagtaactt cttatttttg taattaaaaa
     3841 taaatataat aaa
//
LOCUS       DACULQ010000246         3839 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.248, whole genome shotgun sequence.
ACCESSION   DACULQ010000246 DACULQ010000000
VERSION     DACULQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.248"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            129..1784
                     /locus_tag="JA154_17400"
     CDS             129..1784
                     /locus_tag="JA154_17400"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088528.1"
                     /note="catalyzes the dehydration of
                     2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate
                     in valine and isoleucine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAT7997603.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLNFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            1909..2031
                     /locus_tag="JA154_17405"
     CDS             1909..2031
                     /locus_tag="JA154_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997604.1"
                     /translation="MKTIHLYYKEYEYSIEMYLLDLVEVIQLFKSLMWNAFRSI"
     gene            2128..2760
                     /locus_tag="JA154_17410"
     CDS             2128..2760
                     /locus_tag="JA154_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7997605.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            2783..3595
                     /locus_tag="JA154_17415"
     CDS             2783..3595
                     /locus_tag="JA154_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7997606.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1485 a          443 c          726 g         1185 t
ORIGIN      
        1 ataaaaaatc gtaataaatt ttcattagta tatgctaata tttttttaca attttcgtaa
       61 ttaattaata gaaaaatata ttataataat attatgaaaa tttaaaaaag taagaaagag
      121 gtaatattat gagaagtgat ataaaaaaag gaatagaagg agctccaaag agagcgttaa
      181 tgtatggaat gggtcttact aaagaagaaa ttgaaagacc attaataggt atagtaaatg
      241 cacaaaatga ggtgattcct ggtcatctgc atcttgatga aatagcagaa gctgcaaaaa
      301 atggagtaag aatgagtggt ggtttgccat tagaatttcc tgctataggg gtatgtgatg
      361 gtatagctat ggggcatgtt ggaatgaatt attccctggc ttcaagagaa ttaatagcag
      421 attcaataga agctatggct atggcacatg gttttgatgc tttggtactt atacctaatt
      481 gtgacaagat agtgccaggt atgttaatgg ctgctgcaag acttaatata ccaagtattg
      541 ttgtgagtgg agggcctatg cttccaggta aaaaaaatgg aaaagtatat gattttaact
      601 ctgcaatgga gggtgttgga gcatgtaaag atggaacagt aagtgaagaa gaattagaag
      661 cgttagccat gaactcttgt cctggatgtg gttcatgttc tggtcttttc acagcaaata
      721 gcatgaattg tttaacagaa gctttaggca tgggtatacc atataatggt actgctgcgt
      781 cacattctgg agaaagaaaa aggatagcaa aatatgcagg tatgtatgtt atggagttac
      841 ttaagaacga cataaaacct agagatattt taacaataga tgcttttaaa aatgctatag
      901 ctgtggatat ggcaatggct ggttctacaa atacagtact tcacttacct gcaatagctt
      961 atgaatcagg aatagagctt aacttaaatt tttttgatga aataagtgaa aaaactcctt
     1021 gtttaacaaa attaagtcca agtggaaaac atcatattga agatttacat atggcaggag
     1081 gaataccagc tataatgaac gaactttcaa agataaatgg aataaattta gattgcaaaa
     1141 ccgtaacagg caagactata ggggaaaata taagaaattg tgaaatagaa aatgaagaag
     1201 taatacatac attaaagaat ccatatagta accaaggtgg gcttgcaata ttgaaaggaa
     1261 atcttgctct aaatggagct gttgtaaaaa agtcagcagt tgcagaagaa atgttagttc
     1321 atgaaggacc agcaagagtt tttaattcag aagaagaagc tgtaaatgct atttttggta
     1381 aaaaaataaa caaaggtgat gttatagtta taagatatga aggtccaaag ggtggtccag
     1441 gaatgaaaga aatgctatct cctacatcag cagttgcagg aatgggactt gataagcatg
     1501 tagcacttct tactgatggt cgtttttcag gagcaactag aggagcatct ataggccata
     1561 tttctccaga agctatggaa ggtggtttaa tcgggttagt tgaagaagga gatataattt
     1621 ctataaacat accagacaaa aaattagagt taaaagtaga tgaagttgaa atagaaaata
     1681 gaaaattaaa atttaaacct ttagaaccga aaataaagca tggatactta agtagatatg
     1741 ctaaactggt aacatcagca aatacaggag cagttttaaa atagtagact atatttatgt
     1801 aaattagaaa aagttaagaa aatattaatt ggatatctta ggtgttatag cactaatgag
     1861 gtatcctttt tattttgtat gaaaaacaca gcaaaatctt gattatcttt gaaaacaata
     1921 catctctatt ataaggaata cgagtacagc atagagatgt atcttttaga ccttgttgag
     1981 gtaatacaac tttttaaaag tttgatgtgg aatgcttttc gttccatctg aactatgaag
     2041 aaattgaaag tcagttaatt cttgacataa taaatattaa aatgtataat aaaaaacaaa
     2101 cagacagtct gtttttagga gggaaatatg aatagagaag aaaaaagtaa aaatagtaaa
     2161 gaaaaaatta tccaatcagc attttcacta ttttcgtcta aaggatatga ttcaacatct
     2221 acacaagata ttatcaattt atctggtcta tctagaggtg caatgtatca tcactttaaa
     2281 actaaagaag atatactgag aagtgtcaca aaagaacttt actcacaaat gaataatttt
     2341 ttagagcatc ttgttgctga tgacaccctt acagcaaatg aaaaaataat agaattggtt
     2401 gttcatagtg cgaatgatta cacacgtaga aaaatggtac attgtagctg gttagaaaaa
     2461 atcccattcg ctttaataga ggaagttcgt aatctcaaca atgtagttgc acccaatatt
     2521 gctaagataa ttaaacaagg tgttgaaaat aaagaatttt cttgtgaata tccagaggaa
     2581 ttagctgaaa tgctcgtttt tagtattgat attttacttg accctgtatt atttaaacgt
     2641 gaatacagtg aagtatgtaa taggttggat tttttattat tcatgttaaa aaaaatggat
     2701 attcctctaa ttgatgagta tggtattcaa aaatttaaag atttatttag acaattataa
     2761 taggaaaagc gaggaataga ctatgtttta taatgcaaaa agttgtcaaa tgatgatagg
     2821 aagtaataca gtgaattata ttgaatttgg tagtggtaag aagacactaa ttattttacc
     2881 aggattagga gatggtttat ttcctttaca tggaaaaata caggcgattg cttttgcttt
     2941 tagatataag caatttgcaa aagattataa ggtttatgta tttagtagaa aaaatcaaat
     3001 tacagaaaaa tattccacaa gggatatggc taaagaccaa gcagatataa tgaaaaaact
     3061 tggaattatg aaagcagaag taatgggtgt ttctcaaggt ggaatgatag ctcaatatct
     3121 ggcaattgat tatcctgagt tagttgagaa acttgtgttg gcagttactt cttcaaagca
     3181 aaatgatact atccaaaatg ttatttgtag ttggattgat atggcgaaga agcagaatta
     3241 caatgattta atgattgaca cagcaaagaa atcctattct gaaagatatt taaaaaaata
     3301 ccaattattt attccttttt taggaaaagt aggtaaacca aaggatttta aacgatttat
     3361 tattcaagcg acctcttgca tagaacataa tgccttttcc gaattaaata aaataacatg
     3421 tccaacctta attataggag gtgctaatga taaaattgtt ggaaataatg cttcttttca
     3481 tttagcagaa aagattaaaa aaagcgaaat atttatctat gagggattgg gacacgctac
     3541 atatgaagaa gcacaggatt ttaatgaaag agttttagag tttttaaata agtaaacacc
     3601 tttgttaaaa atatacatat gaagattgga aatttaatgt taaaaataga aacatgaaaa
     3661 tatgcttaac tggtattttt actattcata accaattttt gatacattat ctactataaa
     3721 tacaaatata gcttcaatgt gattatatat tagtgtattg gtaaagcact tatacaaaca
     3781 gaggaatttt gtaaattcat attataccca catttgttaa cttatgaaaa tataatcaa
//
LOCUS       DACULQ010000247         3798 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.249, whole genome shotgun sequence.
ACCESSION   DACULQ010000247 DACULQ010000000
VERSION     DACULQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.249"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            18..485
                     /locus_tag="JA154_17420"
     CDS             18..485
                     /locus_tag="JA154_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997607.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            522..2291
                     /locus_tag="JA154_17425"
     CDS             522..2291
                     /locus_tag="JA154_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HAT7997608.1"
                     /translation="MSMTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPT
                     KACVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKI
                     IYVENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDID
                     FISSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSK
                     QACNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNL
                     KPCSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLY
                     EHNEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYAT
                     KVDDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFD
                     KMGVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDAT
                     YENNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKL
                     DLKNFSSKDNYDDDILIVMLKDR"
     gene            2293..2907
                     /locus_tag="JA154_17430"
     CDS             2293..2907
                     /locus_tag="JA154_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT7997609.1"
                     /translation="MIMDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECG
                     LSCKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSI
                     SAKLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDF
                     FFKSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            2979..3548
                     /locus_tag="JA154_17435"
     CDS             2979..3548
                     /locus_tag="JA154_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization protein"
                     /protein_id="HAT7997610.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         1546 a          336 c          672 g         1244 t
ORIGIN      
        1 taaaatattt aggagtgatg aaaatgttaa atgggataat aaagcttttt tattctgaaa
       61 ttgtaaaaac aaagattaat aacttagatt caagtggata ttctttaaga gatgaatgta
      121 aagaaatcag aaatataact ctaaagatag atagtgtatt ttttaagctt ttaaagttag
      181 cttttggttc aatattgctt atggtagctt ttaatttaaa catggtttta ggtataggag
      241 tgtttttagt tggtgtagtt tatatactat ataaaagaaa tatggaagaa caagtaaaag
      301 agcatataaa taatgtgaaa aataatatcg acatctctaa aataactttt atggatgaaa
      361 aagggagaac aggtctaaat gcattaatta cattacttct tataggatta ttgacaggat
      421 ttaactggat attagttgct agttttgtcg ttgtttttgt ttttactata aagaatgtat
      481 gttaaaatac taataaatag tttaatatat agggggagaa attgagtatg actaatggca
      541 gttataaaat gaagaattta gtggaaatat ccgctatggt gactgaatct acagattttt
      601 ttaatataaa agatgacata atagaaaaaa tgttagaagt tgttcaccct acaaaggcat
      661 gtgtgaatct attttataaa aatgattcta aatatgccta cttagtatgt tcgcaaacct
      721 tagaatatgt acctcaaatt tttccaataa atagtcttaa aggtgcaaaa attgattttg
      781 acacatatcc agagtatata catgaggctg taaatgaaaa aaaaataata tatgtggaaa
      841 atgtatttga agattctaga gcagaaggtg aacgagattt agcaaaagct gaaggttata
      901 ttgggagaat agtttttcct tttataataa ataacgtagt tgttggattt atgacttgtt
      961 ttttaaaaga ggatgattat ataacagaac aagatataga ttttatatca tctgttgcat
     1021 cactcttaag tttatcaata gaaataacta ataaaaataa taatgtacaa attttaattg
     1081 ataaactaag aggctcaata agtgccataa atgaagctac taaaaaacta taccttaata
     1141 agagtattga tggatttttg gaaaatttaa gtaaacaagc gtgtaatatt actaatagta
     1201 aagaagcttt aataattata aatagtcatc ataataagga tgctatagtt agttgttata
     1261 atgttgagca aaaaaagaaa acaaatttat atcctatgat tgatatgttt ttaaagaaag
     1321 agtcattggg ggggtattta aataatttaa aaccatgctc aaaaaaggga ataaatttgg
     1381 aaagttatat atattacaaa cttcaggata aaaatgagac tataggatgt atagtttgtg
     1441 caaatagcaa aaactatact agtgatgatt taaatattct tagtatattg tccaaacaag
     1501 tttctgttgg tatgcaatta tatgaacata atgaaaagga agtaaaacac aaagttcttg
     1561 aaaacgagtt gaacatatta aataaacaac aacagcttat tatggataaa ggtaaaatgg
     1621 attgtaatga tgagaaagaa ctttattttt accataaacc agcaagggta gtaggtggag
     1681 atttttatta tgctacaaaa gtggatgatg aacatatagt ttatattgtt gctgatgtaa
     1741 tgggacatgg aatggtatca aattatgttg ttgcaatgat aaaaggtgct tttaaagtat
     1801 tatgcaatca atacaataca ccaagagaaa taatgacaaa tttaaatagg atgttatatg
     1861 atgaatttga caagatggga gtatttacaa cttgtttgat aggtattata gatactaaag
     1921 aaaatatgat tactgtgtct aatgcaggtc actatagtcc tgtagttgta aagaaagatg
     1981 gcacaataga aaataattta aattgtaaaa agggcatacc tattggaata atggaagatg
     2041 ctacttatga aaataatact ctttcaatgg aagattatgc aatggtatgt atgtacactg
     2101 atggaatttt agagataaaa aattcatcaa aagaagagta tggtataaac agattagaaa
     2161 actttctaaa agaaaatttt agattaaatc aacagttaat agtagaaaat ctaaaattgg
     2221 atttaaagaa cttctcatca aaagataatt atgatgatga tattttaata gttatgttaa
     2281 aggataggta gattgattat ggattcaaac gataaaaaag gacttaaaag tccagagatt
     2341 ataataataa atggaagtcc aagaattaat aaaaattgca gttttatagc aaatgaagta
     2401 aaagctatgc tagaagagtg tggattaagt tgtaaaatat ttaatatcta caatatggat
     2461 attgaatatt gcacagcatg tggattttgt gaaaaaacag ggtattgcaa gtttaatgat
     2521 gatatgaagc cattatataa aatgtttgat gattcaaaag gaactatagt aataagtcct
     2581 gtacattttg attcaatatc agctaaatta aagactgttg ttgatagaac tcaagctata
     2641 tatgcaagta aatatgtatt aaataaacct tcaattgata gaagtaagaa acggatagga
     2701 atgtatattg cagtgggagg gagtaaatca tatgattcac agtttaaagg tggacagatt
     2761 gttatggatt ttttctttaa gagtataaat actaaacttt tacacaatat atatttaaac
     2821 aatacagatg atgtatctta tatagataat caagaattta aagatataat atataaaaat
     2881 ttaaagatat atattgattc aatataattt gattttgtag gtaatattat gtataattgt
     2941 attgcaataa tttaaataat taaaaggagg gaatttcaat gaaattacca atagcattat
     3001 ctaataaaca tgttcatttg agtcaagctg atatagacgt attatttgga gcaggtcatg
     3061 agttaactaa atttaaacca ttatctcaac cagggcaata tgcctgtgaa gaaaaagtgg
     3121 atttagtagg accaaaagga actttaaaag gagttagagt actaggacca gctagagctc
     3181 atactcaagt tgaaatatct ttagcagatg gtttcccatt aggagtaaaa gcaccaataa
     3241 aagaatctgg tgatatagct ggaactccag gtgttaaatt agtaggacct gctggagaag
     3301 ttgaaatgaa agaaggagtt atagttgctt ctagacatat acatatgagc aatgaagatg
     3361 ctgctaaatt tggtgtaact gataagcaaa tagtaaaagt taaaacatct ggaccaagag
     3421 cattagtatt tgaaaatgta ttagtaagag ctagtgacag ctttaatcta gaaatgcatg
     3481 ttgatatgga agagggaaat gcagcaggcg ttaaaaatgg agatttagta gaattaatag
     3541 ttgattaata ttataaatag actttatgct agtagatatt ttatttgcta gcatattttt
     3601 aaatgtaata aatttctata ggataaattt aatcaattag attaatttat aaaataaagg
     3661 ggtattataa aaaatgtaat aaaaacttac aaaaatatga tgtaatatgg taaaattata
     3721 tatataaaaa tttacatgta ataaggataa gcattttact ttttatacta atatgaacaa
     3781 atagataaaa aagtttga
//
LOCUS       DACULQ010000248         3753 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.250, whole genome shotgun sequence.
ACCESSION   DACULQ010000248 DACULQ010000000
VERSION     DACULQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.250"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(156..488)
                     /locus_tag="JA154_17440"
     CDS             complement(156..488)
                     /locus_tag="JA154_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAT7997611.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(732..1373)
                     /locus_tag="JA154_17445"
     CDS             complement(732..1373)
                     /locus_tag="JA154_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997612.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFVYSYLMYKISGKNN"
     gene            complement(1354..1650)
                     /locus_tag="JA154_17450"
     CDS             complement(1354..1650)
                     /locus_tag="JA154_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997613.1"
                     /translation="MLGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATI
                     SYHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            complement(2271..2405)
                     /locus_tag="JA154_17455"
     CDS             complement(2271..2405)
                     /locus_tag="JA154_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997614.1"
                     /translation="MNISRRAMKIIELAQKIANKRGVTVQDAWNDAMKEYKEKYEYVA
                     "
     gene            complement(2616..3368)
                     /locus_tag="JA154_17460"
     CDS             complement(2616..3368)
                     /locus_tag="JA154_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997615.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VAMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(3427..3696)
                     /locus_tag="JA154_17465"
     CDS             complement(3427..3696)
                     /locus_tag="JA154_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7997616.1"
                     /translation="MNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLL
                     DRSMSVSDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
BASE COUNT         1394 a          590 c          329 g         1440 t
ORIGIN      
        1 tgtaaataat ttaaaactac tatattattt aaaaatttat atttatatat gcaattctaa
       61 aaacttaata agaagtgaac ttagaaaata aagcttaatt tcaaaggctt aaatagacta
      121 ttattattag tctaaataaa ctacgtgtat caaaattata tattaaattc ttctaaaatt
      181 tttattgcaa atcttgcgtt tggatatgta atagaccctc caccaattac acctattttt
      241 atagcttcta taatttcatc tttacttgct ccagaattaa tacaaccctc aatatgataa
      301 caaatacact catcacatcg gcttaaaatt gaaatagcta gtcccattaa ttccttattt
      361 tttttactta tagccccttc tgaataagtc ttatcatcaa ggcttccaaa tactctaaaa
      421 aactcatctg tttcacttaa ttttttattg taaaatcccc tatattcttt catttctcta
      481 gtattcatct tctacctcct atttttttct tataagagta tcacactgta cttatatttt
      541 caatattaaa tacaaatttt cattttatgt tcattagcct aatttatatt attttttgaa
      601 atctcaataa gttatcatat aagtaagatt ttttcagaac taaaatagaa atctcagtaa
      661 tttctttatt atctatctca gttctgaaat atattattct tatccaaaca ttattatagt
      721 gattaattat attagttatt tttacctgaa attttataca tcaaataaga atatacaaat
      781 ggtagtatta caattacaaa gattacaaag atattataaa aaggaatatt aataaatgca
      841 ttaataatta ataatattcc accaataagc caaatccatc cagctaaatg gtgtgtctta
      901 ttccaattat cttcatcatt aagagtccat ggtaaaatta ttcctactga gtaatttcgt
      961 ttacatttag gaagatagtt tccaaataaa ataactatta ttcctaaaat taatcctgta
     1021 taaaaattag actccaaact aactcctgtt gaatataaaa ccatcattat ttgtataatt
     1081 actgaaagta tagcaactcc ccattttgat aaattttgta taacatttga aaaattagct
     1141 ttcttaggct cattctcaag tctaaaccat gtatacaaat ggatacatag cattaacaat
     1201 ggtacaccaa aagctgctat aacctttgga gcataattat ctggatttcc ataattatca
     1261 aaatgaattg caacttcatt tggtaatcta ttataaaaat atattgatac aataaatggt
     1321 ataaaacata ttaaagtaga taaaataaat tgtttattta tcgctttttt cattatcttt
     1381 ttctcccttc tccataaatt gcgaaaacca aagcataacc tcttcgaaaa ctgaaatatt
     1441 tatttcgtag aaaataaagt tcttatactt tgtttcaaat atgagtcctg ccttttttag
     1501 ttgagataga tgataagaaa ttgtagcatt cgtcatatta aatttttcac taatttctcc
     1561 tgctgacatt ttattctccc tcaacataac caatatttct cttcttatag ggtctgaaag
     1621 agctttgaat gtttctggaa accccaacac catcacctcg ctatttagaa tttcatctaa
     1681 atagaatata acataacatc cattttattt caataactat ttagaatttt ttctaaatag
     1741 ttatttctaa atatttattg aaaattttca tattaagatt ctcaatttac ttaagtcgct
     1801 tttatttaat cttactttat ctaatttttt taacttaaca aaagtgtatt gcaccataat
     1861 tgttaaagca aaaacctaat gactgaggta cttatatata taacaatact tttaatttta
     1921 aattaaaagt agctaaaccc aatttaaaaa gtagttaagt cctatttaaa tagaaaagaa
     1981 tgaaacgaga aatatactgt ataaaagtac ataaaaacat ctactaatta gatacaacta
     2041 taacagaata tatttattat tataataaca aaagaataaa aaaaaattaa tttagcatag
     2101 ccttgttaaa tatagatttg aatatgaatt taaagttatg taaaattaaa aatacaggaa
     2161 tgaaaattcc attcctgtat aaaatctaac ttttgagtct cagtacaatc ttattttgag
     2221 acaatctttt ttgttttata ttttcttata tttaaaatat aacgcatatc ttatgcaaca
     2281 tattcatatt tttctttata ttctttcatc gcatcattcc aagcgtcttg tactgttaca
     2341 cctcttttat tagcaatttt ttgtgccagt tcaattatct tcatagctct tcttgatatg
     2401 ttcataaaca ttcctcctac aaattaccat atatttcata tatataaatc aaggttatat
     2461 ttttatgata tataatcctt ctattgtaag attacagttt atatgtgtat tatatatgaa
     2521 ctatattaga agataattag aatttttata aagctatatg attagattaa atataaaagc
     2581 tttacaatta cttataaatt taaaaagtct ataaactaaa ctaatgtttg atataccaat
     2641 tttacgacca taaataatgc cattactaaa aatattggtt ttatcatctt tgaacctttc
     2701 tttatagcaa ctttagcacc tacataagct cccattatca tcactaatgc aaacactata
     2761 gcaattttat aatctacttg accatttatc atgaataata acaatgaagt tatattactt
     2821 gttaagttta atatttttgt atttgcagaa gcatgtaaaa aatcatatcc atatattttt
     2881 ataaatccaa aagtcaaaaa agttcctgta cctggaccaa aaaaaccatc ataaaaaccc
     2941 attataagag ccataagcat ccctagtctt aaatttttct tattaacagc ttcaaaatta
     3001 tcttcttgac ctaaattttt agaaataaag gtgtaaatag ccactattaa aatcatagcc
     3061 acaaccaaaa catttaatat ctcttcactt attgataata cacatctcac acctagaaca
     3121 ctaccaataa tagtaaatgg tactaatttt tttaaaaggt tgttattaat ttttccagac
     3181 tttgcatatc tataggcact acttatacat cctgctgatg ctgcaaattt atttgtacca
     3241 atggcaagat gtactggcac tccaattgcc ataagaacag gcatgcttat aagtcctcct
     3301 cctcctgcta tagaatctac aaaagcagct aaaaaccccc ctatacataa aaaaataact
     3361 gaaaacataa aattcctcca tcattctctt tcaatttttc ttacaatata tattatataa
     3421 tcttatttaa tacttattat ccagcatatt ctttctatat tcatttggag ttatactatt
     3481 aaacttctta aatatttgtg aaaaataact actgttatta aaacctactt tcttacaaat
     3541 atcactaaca ctcatgcttc tatctaacag atatttttta gattcttcta tcctaagaag
     3601 atttatatac tcagttattg aaactttatt tctttcatta aaaatagaac tcaaataatt
     3661 tggacttata aaaaaaacat ctgatatact attcaaattt atattttctt tataatattt
     3721 ttttatatat tcgtttatat tttcaattag att
//
LOCUS       DACULQ010000249         3743 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.251, whole genome shotgun sequence.
ACCESSION   DACULQ010000249 DACULQ010000000
VERSION     DACULQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.251"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            3..863
                     /locus_tag="JA154_17470"
     CDS             3..863
                     /locus_tag="JA154_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997617.1"
                     /translation="MAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTTFMRI
                     LATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVLSDIP
                     LRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEPTAGL
                     DPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEALAKL
                     AEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPTIEDG
                     YMLIMGGDNI"
     gene            860..2149
                     /locus_tag="JA154_17475"
     CDS             860..2149
                     /locus_tag="JA154_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997618.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2169..2723
                     /locus_tag="JA154_17480"
     CDS             2169..2723
                     /locus_tag="JA154_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997619.1"
                     /translation="MLCMIPLIQGVANLDRISTAICLENYVAIIGIVMLVPVFSLEQS
                     KEIDEIVSSKALSQVIVQLIRILMSIIGIVVLIIGFVNVLIYLGCNFPVVEYIIGSFA
                     SAMFIGSLGMFFSNLFNNTIIGYMISIGYLILNMMTKGKYVGKFFIMSMSQGSFDEKY
                     WLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(2990..3453)
                     /locus_tag="JA154_17485"
                     /pseudo
     CDS             complement(2990..3453)
                     /locus_tag="JA154_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1398 a          448 c          576 g         1321 t
ORIGIN      
        1 aaatagcagt atccaatctt agtaaaagat ttgggaaaaa acaagctctt gataatataa
       61 atcttgaaat cgacagtggt atgtttggtc ttttaggaag aaatggagca ggaaaaacca
      121 catttatgag aattttagcc actttattat caaaaacaga aggttcaata catatgtgtg
      181 gagttcctat tgagaatact aaggaaatta gaaatatggt tggttatttg ccacaagact
      241 tttcaatgta tcctaacatg acagtatata aagcaatgga ttatttagca gttttatcgg
      301 atataccact aagaaaaaga agaaaaatta tagcaaagtt attaagcaaa gtaaacctta
      361 ctaattatca aaatgtgaaa gtaaaatctt tatctggagg tatgaataga aggcttggga
      421 tagcacaagc attaattcat aatcccagag ttttaatagt agatgagccg acagcaggtc
      481 ttgacccaga agaacgtata agatttagaa atttactatg cgaaattgca aatgatagaa
      541 tcgttatttt atctacacat attgttggag atatagaagc tacatgtgaa aacgtagcta
      601 tattgaatag tggaaaagtc atatacaatg gctcaacaga agcattagca aaactagctg
      661 aagggaaagt atatagtatt gaagtagata aaaaagatat tgaaaatata aaaagtagat
      721 ttatagttat aggaatgttg actcatggtg gaaaggctat acttaggatt atatcagatg
      781 ataaaccttt tgagacagca gtaaattgta atccaactat tgaagatggc tatatgctta
      841 taatgggagg agataatata tgaatctatt tataaaagaa tgtaaaaata tattaaagag
      901 tatgatatat tatattttta ttgtagtttt agtttttttc tatatttctc aacttggaag
      961 ttcagtttca aatgatgtaa gtaaaaatat tcctaatgat ggaaatccat tagttgctcc
     1021 acaaaaagta gagaatattg aggaatatta ctctgtacac aatcaatatc catatggtca
     1081 aaaagaatct atgaaaacag aaaaagtcat tccaaatgca actataaacc taattagaga
     1141 atatcaaaaa aacacatata caacgtatcc aacaggggta gttaagtata taaaattaga
     1201 ccaaaataaa acgaagacca tagaaaaata tatagaagaa ataactggta aaagtattag
     1261 cgaaataaat tcactttgga aaactgatga aagtataaaa atagattatt ctaaagtatc
     1321 tcttgaaaga tttgaagaaa taatgcaaag agtagacgac attttaggag gagggtctgc
     1381 atatgctttt gacaagttac aaagatttgg agtagttcca gttacctatg atgaagctat
     1441 gaagaattac aactatacaa tacaaaaaga taaagttagt aatgggtatg caagaatttt
     1501 ttgcgattat ataggtatag ttttaagtat atttccagta tttgtggcag tttttatgtc
     1561 acttaaagat aagcgttcaa gaatgagaga gattatttat tcaaaacaag tatcttcatg
     1621 caaaatagtt ttatcaagat attttgcact tgtctttatg atgatattgc ctgtattaat
     1681 tttaggtatg aaagatttac ttcctttgat tttctttgct aaagataaag gtatatctat
     1741 tgataactta gcatttataa aatatacatt atggtggata ctaccaactc ttatgatagt
     1801 tacagctgta ggaatgagta ttactatatt aacagataca ccagtagcaa ttattgtgca
     1861 attaatttgg tggctttaca gtataaattc tacaggactc aaaggtgatt attcaacaat
     1921 ggggctaatg ataagacata atagtgtatt aaatggggat attattatac aaaacttcaa
     1981 tcaaattatg attaatagaa ttgtgataag tttgggagca ttggtgcttg ttttgttagc
     2041 tatattctta tatgagcaaa aaagaagggg gaatcttgat gtttgggcaa agttttcaaa
     2101 actgtttagt tctaataaaa gaaaacttca aattaagcac tttaactaat ttaatatgtt
     2161 caattatatt gctatgtatg attcctctaa tacaaggagt agctaattta gacagaattt
     2221 caacagctat atgtttagaa aattatgttg caataatagg gatagtaatg ttggtacctg
     2281 tattttcgct agagcaatca aaagaaattg atgagatagt atcttcaaaa gcattatctc
     2341 aagttattgt acaattaatt aggatactga tgtcaattat aggtatagtg gtattaatca
     2401 taggttttgt aaatgtttta atatatcttg gatgtaattt tccagtagtt gagtatatta
     2461 ttggaagttt cgcatcagct atgtttatag gaagtttagg aatgtttttc tcaaatttat
     2521 ttaataatac aatcataggt tatatgatat ctataggata tttaatttta aatatgatga
     2581 ctaaaggcaa gtatgttgga aaatttttta taatgtctat gagtcaaggt agttttgatg
     2641 agaaatattg gctgatactt ggaagtgttg tgttgataat tgcatctctc ttaataaaac
     2701 cagtaaaaag gaagattaga taataaaatg agagcttaga taaaataaag gtaatgcaaa
     2761 taaaaagtac atttatgaaa tataaatcta tttttatata cacaaaccta cttacaatct
     2821 ctttatgtat gtagatagat ttatatatcc gtagtataaa atatatatgt ataccgaatt
     2881 ttatcaaaag ctctcaaatt tatatttcaa agacacttat tgctatgacc tacacatatg
     2941 tattttttca ttttaattgt tgtattaaca ataggactat tgttataaac tattgatacc
     3001 tcataacaaa aaattctcca tctcctaaat gcgttttttg taccttagtc tcaactattt
     3061 taaatccttg agactcataa cattttacag ctctcatgtt ccaggttctt acatttaata
     3121 caattggttt agtactatat ttatttctac attcttgtaa tcctaggcta attatttctt
     3181 taccaattcc cattccacaa taattcggtt tcaaccaata ccaaagaata cagagcttcc
     3241 ttcatctaat aagtttataa aacctatcaa ttctttattg tcatttataa aacttataaa
     3301 attttttctt ttatcttctt ttgctagtga aaaattcttc tttttaattt cctcccatgg
     3361 aggtagattg tatattgaat atttaccttc atatttccaa ttagtaactt caatagcatg
     3421 ttcttcagta aatttaactt tatttaaatt catatgtcct cctattaaaa tatttaaggt
     3481 aattaatggt attttaaagt tttagaaaat aagtaaaact atttatattt atccataaaa
     3541 tctttaaaaa ttttactaac aattcttgta ccctgtactt tttattggat tcacagaaga
     3601 ctataaacta attttgaatc accttctaat tcaaaaacat cacgatatat ataatttctt
     3661 agcttgatta aattctgtta aatccgtttc ttatagttgt ataatcaaca tcaatatctt
     3721 tatgtgtatt agttaattta ttg
//
LOCUS       DACULQ010000250         3725 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.252, whole genome shotgun sequence.
ACCESSION   DACULQ010000250 DACULQ010000000
VERSION     DACULQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.252"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            90..455
                     /locus_tag="JA154_17490"
     CDS             90..455
                     /locus_tag="JA154_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997620.1"
                     /translation="MKIRAEYTCLLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            533..1087
                     /locus_tag="JA154_17495"
     CDS             533..1087
                     /locus_tag="JA154_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAT7997621.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            1307..1981
                     /locus_tag="JA154_17500"
     CDS             1307..1981
                     /locus_tag="JA154_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAT7997622.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      2336..2432
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2486..2662
                     /locus_tag="JA154_17505"
     CDS             2486..2662
                     /locus_tag="JA154_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997623.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(2850..3089)
                     /locus_tag="JA154_17510"
     CDS             complement(2850..3089)
                     /locus_tag="JA154_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997624.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(3223..>3725)
                     /locus_tag="JA154_17515"
     CDS             complement(3223..>3725)
                     /locus_tag="JA154_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7997625.1"
                     /translation="IKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDF
                     LIQKLQKEYEDRFNILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDE
                     GYYVTLNYTGSNSNNKIYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEI
                     QVPITK"
BASE COUNT         1385 a          507 c          465 g         1368 t
ORIGIN      
        1 acataaaatt tatatacagt tcatatatat ttgtcatatc acaagacaag tgatataatt
       61 tattatcatt ttactggggg tgatatgcta tgaaaataag agctgaatat acatgtcttc
      121 ttgaaattgt acatgacata atcaaaggaa aatggaaaac tatccttata tttgaattaa
      181 gacaatctaa taaaacattt tctgaattgc aacataatat ttatggaata aatcaaaaaa
      241 tgttgattca acaattaaaa gagttacgtg attttgggat tattgataaa ataagttctg
      301 ctggatatcc attacatgtt gaatattttc ttactaatcg tggtaaaaaa atgttaaaag
      361 cagtggaaat aatgcaagaa ataggaatcg aatatatgct tgaacatggt caacaagaaa
      421 ttctagataa taaaggaatt tgctacaata agtaatccta tacataccaa aaagtaagta
      481 atttacattt tttacaatag tattataata aaaataaata ggaggtataa tcatgtctat
      541 agctttaata ttgattgata ttcaaaatga ttattttaaa aatggaaaat gtgaattatt
      601 tcaatcagaa gaaactgctg aaaatgctaa aaaaatacta ctgtttttta gaaaaaacaa
      661 tttaccagtt attcatatac agcatatatc aacagataaa acagcttctt tttttcttcc
      721 taatactcat ggttcagaaa taaataaaat tgtttttcca ctagataatg aagaaattat
      781 aactaaacac acaccagata gtttctttga aacagatttg caaagtatcc ttgagaaaaa
      841 aaatattact aatctagtaa tctgtggaat gatgagccat atgtgtattg atacaagtgt
      901 aagaacagca aagaaacttc gatacgacat aactctaata agcgatgcat gtacaacgaa
      961 gaatttatct tggaataata tagaaattaa tgcaaatact gtacatcaag tattcatggc
     1021 atcactacag aacagttttg cagatgtaaa aaacacagat aaatttttat ctaactatca
     1081 caaataaaaa catacactat gttaaaagag caagtttgat ttatttaagc ttgctcttta
     1141 atcatttaaa attagatgct ttctatcatt tttatataca taatatacgt ttatacattt
     1201 tatattcata tataactatt atataaattt taaactttac tatattcaca taaaaaatgt
     1261 agtattatag tatatataat attatataga agggggtgct agataaatga accatcatat
     1321 attgattcaa tttgaatata tagcaagaat cattactgct ggtttctgtg gggctttaat
     1381 tggatatgag agaaagaata gactaaaaga agctggaata cgcactcatt ttatagtggc
     1441 attagcttca gcacttataa tcattgtttc caagtatgga tttaatgata tagtgggtac
     1501 tccaggtatt ggtctagacc cagcccgcgt tgcagcacaa gttgtcagtg gtataggttt
     1561 tttaggtgct ggattaatat ttattagaaa tcaatcagta agtggtctta ctacagctgc
     1621 tggtatatgg gcaacagctg gtacaggtct tgccattggc tctggattat acttagttgg
     1681 tattgtttca agtatcatta ttgttattgt acagataaca cttcatagag ataggaaatg
     1741 gatgaaactc cctacagcag agcaagtttc tcttaaaatt tctaacagca ctgattccat
     1801 agaatatatt gaaggtaaat ttaaaaaatc taatattgaa attataaaca tgaatattaa
     1861 acgccttgat ggggattggt tagaagtagt tatatatact aagttaccta aaggatataa
     1921 aaaaacaaat ttattaaaat tatttagcga taatcctctt atagaaactt tagaaatata
     1981 atataaaaat tgaagcttaa aaataataat atatttaatc aaggtcaata tataaataat
     2041 tacgggatga gtctatataa aattcatccc ttgttttttg ccattcatat atattttttc
     2101 aagatatttt tgttcatcta ttatctagcc aagtaactct tgtgaactaa actttttaat
     2161 acaaaaactt caatacaaaa ctaaatataa tttttattaa atattctaca tgtatagttt
     2221 atttcttgag tgaaaaatag atttcatatt gttaagatac tgttaaatat ttcaattttc
     2281 tgacaagtat gttgaacaat aacttatcct agtgtattat tttagttgta gaataaaact
     2341 aaatcggcaa aactagagaa atttagtgac gcaaagctat agggactaac gcttataaaa
     2401 taaactatga gttatgtcag ccagttgcca aaaagatatt gttctttttg ttttatgaaa
     2461 gttttttagg gggatagtaa tatatatgtt tagagatgaa atggataaat gtacacacat
     2521 gttaactgct tatatcagta gtttatatga ttattgtgat tttatagata cacagttaga
     2581 tgattttata ctagagtacg gagaaaatgt agtagaatct tgtttacatc aagtgatggt
     2641 attggtaagt aagtataatt aaatagaaat tgttttctag agaacccttt ttagttttat
     2701 ctacatttaa aaatgttatt atattaaaaa actaatctcc cctatatgca aagttttctt
     2761 ccatataaca atttcttccc atttatactt tttcacttgt atctttaata aaattcaact
     2821 tcaaatatta atatacttca aaaattattc tatttcttat ctttacacca attcctatca
     2881 gtattatcct ttatataggt tctaactatt tctccacaat ctaaacataa atctgaatat
     2941 atagtagatt gtactggaaa agttttattt ataactttat ctaattttcc atctcctcta
     3001 tattttaatc caatagtttc agcatatatg ccaccagttg ttaaaattcc cttttcaata
     3061 tttacacttc cacaattaga acatttcaca tttataaccc cctaaacaat tattttttca
     3121 tgctatctta taatttaaaa tataatttgc taccgatttt ataacacagt ggcaaatcaa
     3181 cattttttca ttctatttac catctaatcc attgaaatat agctatttag ttataggaac
     3241 ttgaatttcc gttacaaact cctctacaat tccagtctca tgtatatcta tcttatagat
     3301 ttctataggg tcagcaatta tcttataatt attttcttct ataaatttaa acaccttctc
     3361 catatatatc ttattattag aattactccc agtataattt aaagttacat aatatccttc
     3421 atcaaaaact atattatata ctttctcatt actttccaga aaacaaaata cagatttaaa
     3481 ctcattaaat atacctttat taattgcttc agtagaaaag gcagaaccaa tattgttgtt
     3541 tccaagtata ttaaatctat cttcatattc tttctgaagt ttttgaatta aaaaatcgaa
     3601 gtcttcatcc ctctttatat cagcatttaa ttctaaagct tttctttttt ctatatatac
     3661 aacctctatc tcatctattt ttgtattttg aattacactt tcaattgaac tcaatctttt
     3721 tataa
//
LOCUS       DACULQ010000251         3712 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.253, whole genome shotgun sequence.
ACCESSION   DACULQ010000251 DACULQ010000000
VERSION     DACULQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.253"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(168..704)
                     /locus_tag="JA154_17520"
     CDS             complement(168..704)
                     /locus_tag="JA154_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HAT7997626.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(722..2035)
                     /locus_tag="JA154_17525"
     CDS             complement(722..2035)
                     /locus_tag="JA154_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate
                     sulfurtransferase"
                     /protein_id="HAT7997627.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2390..2830
                     /locus_tag="JA154_17530"
     CDS             2390..2830
                     /locus_tag="JA154_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997628.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(3015..3203)
                     /locus_tag="JA154_17535"
     CDS             complement(3015..3203)
                     /locus_tag="JA154_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997629.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
BASE COUNT         1279 a          640 c          359 g         1434 t
ORIGIN      
        1 tatataattt tattttttct aagagtataa ttataaaaat aaaaaaagaa taggataatt
       61 ttcctactct tttttctgta cttacatata atattaaaac tctttatttt ttaatatata
      121 ttttaaattt tcttttttaa taaatctttt tcttttaata aatctattta attttcttct
      181 tctatatcag agccaaaggg aatccatgca ttaccaaact ctttatcttt aaataaataa
      241 gctaatccag ttatttgctt caatctaaga atttcatcaa cttccatacc caggtgcttt
      301 gatatccatt catcagattt tccaatttca caaagttcag ttataatttt actcattaaa
      361 tccacattat gagaacctct tgcacgattg tgtcgaatag tacttgccat tctttcatca
      421 agagatttat caattactga aacaggaaga agtccatttt ctctatcata tatatcctca
      481 ttttcaagca ttactctata tctatgaaaa ccatctacaa taatatactt atccttttcc
      541 tcattataat aacagacaat tggcattgta tacccatctt cttttatact tttatacaac
      601 aactgcattt ctggtggggc tactgcattt ggattatagt cgttagattc tatcttttcg
      661 attggcactg cgatgattcc atatacagga cttttatatt ccatagcatc ttaactccta
      721 tctaatgaat tttttcatat ttcttcttaa tatattccgt tctaattctc tgttcttttg
      781 taggaccaaa tcccataaat ctacacatat ggtcattttt taaaatacaa taacacattc
      841 ttttccaact tggtatatct ttggtaaatt taatatcatc cgtatcatca ggtatatcat
      901 ccaaaaatat gactctactc tttttatctt ttgtatgatt tgaaactcca tttaattcta
      961 ttttataacc ttttctcaac aattcacgta taactatttc tgacaaacca ccacctgtat
     1021 tctgccaaaa ctcaatagat gttgaaaatt ttttaatata gctattcctt atattttcag
     1081 gtaaggtatc aagcaaaaaa tatgtgaaac ttttccatgt gtgtccatat ggcaatttaa
     1141 cactacgata tcccatagct tttgttttgc cataaataga agtaaaatta actccttgta
     1201 ctctagcaac caatttcacc catacatctg ggtccaatat tctgtataaa tttaaactct
     1261 cttttgctga atcattaaaa ggtgatgcca ctctcatttt tcttggagct actccagctt
     1321 tatagaataa gtcataaatt ttattatagt catagtcaaa tttatagtgt gctaaccata
     1381 tatcttctat ttcccaatca tatataggag atgccgtcca gacattttta aattccttag
     1441 ttatccagca ctttccttta tatccatgct gtttattcac aatagaacaa taacgttgta
     1501 gagattctgc tgctcgtgtt cctaataagc aaacaacttt tgcaccttca tgtatatctc
     1561 taatccatct accaaattgt tttgccaaac tttcttgtct cattttatat ttataataag
     1621 taaaaggatt atttttttga tttattatgt attctttgac tggcatttcc cttacccata
     1681 actcttcttt ctcatcatcc catggatacc aatacatttc ataatttcct acagcagttc
     1741 ttgtagccat tggtaaacac acccaatatg gttcaatatc ttcaatatta tcttcaaata
     1801 ctttttcaac atattcagta gtcaaactat attgagcctc aaaatcttga tgaaatactc
     1861 ctattttgga acttatatta ttctttctct tataatccaa aactaaattt agcagtaaac
     1921 cactatcttt tcctccgcta aaagatacat agatataatc aaattcatta aaaagataag
     1981 aaagtctttc ttgaaaagct tcaaatacat cggattctaa atatctctta tccatttgaa
     2041 ttatcccctt tcaattagaa gtttataaaa ccttacaatg caatattatt atgctttttt
     2101 agcagtaatc agcataaatt atacattatt taaatacaca aatccaacta actacatata
     2161 attataatgt aattatactt ttgtattata aactatattt tatcattatt ttacattttt
     2221 ttccaatata tataaatatc atatttttct attagctaaa actaattact ttatatttct
     2281 acgtattagt ataataatac ataaaaactt gaataatgca taaaaacttg acatactgtt
     2341 gtcataatta ctttgctata attaaaagta aaaatggagg taaattctaa tgtatgaacg
     2401 tatgcttgat aaaagcaata aaccctgttt gtctcaaata atagaatata taggtaaaga
     2461 tggatatgca cgattgtgta agttggaaac ttacttgaaa actcaatata atttatcaag
     2521 agaacttcga tttccttttg gaaacaatta tggttggggt tacaaatata gccataaatc
     2581 ttttcattta ttttatgctt tttttgaaaa aggtgcattt actgttctga ttcagatagg
     2641 tgacaagcaa gttccagcag tagaaaaaac gttggtagct ttgacaccaa aagctaaaga
     2701 gctctgggtc actcgttatc cttgtggcaa taatggaggc tggattaagt atcgtatttt
     2761 agaagatgca gacttacttg atattattca atatataaga gcaaaaaaat ctccacctaa
     2821 acaaaattaa cttttatttt ttgtgattta aatatagtgt atataaattc cattctttcc
     2881 atataaaaaa aataatataa aaaatatcta ttagtataac ttatttagta tgtcttattc
     2941 tactttttac agataattaa aaagttttat cataaataaa tcataactag acatcctagt
     3001 aaatcaaaat taatttattc ttttttatta gaatttttga taacagtccc tatacatatg
     3061 ccaattatca ttccaaaaat tagaccataa cacattgtga tacctacatt tcctttagaa
     3121 atacctatag cacaacctat agccattcca agtatcatgc ctataaacat acatataata
     3181 actaaattgt tgtttttatt catagttttc tccattcaat atattttaaa ttgcctagct
     3241 cgccttctta gcttaaaaca aagaaatata aattaacttt ctttcattct aatatctgct
     3301 tgtataaatg tcaaacaaca catttcttgt cataacctcc tctttttgaa gtaagtacaa
     3361 tctcccagac gcaataatat caatttccta aaaccattta agtaactttt atatttacat
     3421 aatatttaca atcataaact atatttttat tcatttatac atcataaaca ctccctacta
     3481 atcattatta tacttaaaat aatgttcatt tataataata tatctaacac actttaattg
     3541 aaaaaaataa aactttgcct ttcattccca tatataaata agtatacttt ttgatgctct
     3601 attacaaatc aactaaatta attgatataa attatcattt tctgttgaaa ataccattcg
     3661 cacatgctat acttaattgc ataaatggta ataaatgtca tatgatttta ca
//
LOCUS       DACULQ010000252         3589 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.254, whole genome shotgun sequence.
ACCESSION   DACULQ010000252 DACULQ010000000
VERSION     DACULQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.254"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..470
                     /locus_tag="JA154_17540"
     CDS             <1..470
                     /locus_tag="JA154_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HAT7997630.1"
                     /translation="NLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRL
                     LKKIKIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDY
                     VLGNIRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            454..852
                     /locus_tag="JA154_17545"
     CDS             454..852
                     /locus_tag="JA154_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YtfJ"
                     /protein_id="HAT7997631.1"
                     /translation="METTLETIKGSIDANTIIGDPIKTDTTVVVPISKVTIGFGIGGG
                     EYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETRIMSLDSNINL
                     VDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1100..1540
                     /locus_tag="JA154_17550"
     CDS             1100..1540
                     /locus_tag="JA154_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7997632.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1556..3007
                     /locus_tag="JA154_17555"
     CDS             1556..3007
                     /locus_tag="JA154_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAT7997633.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKIKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3098..>3589)
                     /locus_tag="JA154_17560"
     CDS             complement(3098..>3589)
                     /locus_tag="JA154_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997634.1"
                     /translation="SSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSLV
                     CSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAIC
                     FIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKKK
                     KSK"
BASE COUNT         1492 a          324 c          637 g         1136 t
ORIGIN      
        1 ttaatttaat aaatataaat agacaacttt atccagctga aaatagtaaa aataatgata
       61 aaaaagaggg gatgaagaat aatatagatt caagtatatt attagctgat gaccttttga
      121 gcatttatag attattaaaa aagataaaaa tacatgaact ttattcaaat attaattttg
      181 gaactggaaa tattggctta acatcttctg tatatgtatt gataaatact ttgtatggaa
      241 atttatttaa tatgattgat gcagaaaaaa tgtatttaaa tgtaaatcca gattttacaa
      301 aagattatgt tttaggaaat ataaggatac atataaggcc aagaataaaa gctttattta
      361 atattataat aatgattaat aaaattatga ataaaaataa aggaaataag gaaggtgata
      421 gtaatgagag caacaggttc gatacagagt cttatggaaa caactcttga aacaattaaa
      481 ggttctatag atgcaaatac tattatagga gaccctataa aaacagatac tacagtggtt
      541 gtacctatat caaaggttac aattggcttt ggtataggag gaggagaata ctcaaaagga
      601 tatgatgata aagataggga aattgaatta aaaaatgaaa aatcagatac gaattttgct
      661 ggaggtagcg caggggctat atctgtacaa ccagtagcat ttgtagtagt tgaaagtgga
      721 gagactagaa taatgagttt agacagtaat atcaatctag tggataatat attatctata
      781 actcctagag tattggagaa aattcaaaat atatcacaaa gtaataagaa tcaagataaa
      841 aacaatatgt aagaataaaa tatttttaaa tatgtatata gttttaggta aagtagtggg
      901 gttagtataa atcccactat tttgttgtgt actttaaaca tttatatcta ataagatttt
      961 agtttaaaac aatttaaata gataataaag ttgatatata gtataattat aaaaagaaaa
     1021 gaagtattat attatagata tataaacgca tttgagatat aagagggagt tataaatcat
     1081 tagttttgag gtgaatgtta tgagcgataa agaattatct attttgttgt tagaaagtat
     1141 gaataatttt cataatttaa taaagattat taataacgaa agatataaaa aagataaggt
     1201 tcttacagaa agacagttct ttgccttagt aaaaataaga aaacatgata aaatagagct
     1261 taaaaatcta agtagagatt tgcatgtatc tacttctagt ctgtgtatac tacttaataa
     1321 attggttgag caagagtatg tatataggga agaagatagt agagatagac gaaatacttt
     1381 ttatggaata actaaaaatg gagaaaagat attggataat gaaatactaa agtttgtatc
     1441 tataataagt gataaaatgg attgcctaga tatagataat aaagataaat tatttacttc
     1501 tttggaagag tctaaaaata taatagaaca attattttaa aaaggagaat ttattatgaa
     1561 tataaagtat tctaaagaaa tattaaaaag tttagaaaat gctataacag tagtagctct
     1621 atttgaggga gaagatgttt ctacgacatt tgataaaata aatatgataa gcagaaattt
     1681 taattctaaa gaaaatagta tagatactgt aacattgatt gaaaataaca atttggtaaa
     1741 tgtttgtctt gttggttttg gtaagagaga gttattgaat agagaaaaaa ttagaatcat
     1801 aggtggtaat ttgtgtaaaa aattaaaaaa tatattgaaa aatgaagcat ataagagtag
     1861 tgatgttaat gtactgaatc ttaatttaaa tagtgatgaa ataggttctt ttgtagaagg
     1921 gatattactt ggaaattata agtttgataa gtataagaca aaaattaagg attttgaaag
     1981 aaaagaagtt gatactataa ctatatttac tgaaaaagaa gttgagtgtc aaatagaaaa
     2041 ggctaaaata ttagctaatt caactataat agctagaaat ctagttaatg aaccatcaaa
     2101 cgtaatatat ccaaaaacat tagcagtaga aacagttaaa cttggtgcag agtttgggtt
     2161 taatgttgat gtttatgaag aagaaaaaat aaaggcattg ggaatggatg cttttttcgc
     2221 tgtttcaaga ggctcaatta ataaaccaag attcatagta atgagatact ttggagataa
     2281 agaaagtaca gatatattag gtttagtagg taaaggattg acttatgata ctggtggata
     2341 ttctttaaaa tcaaatgctt ctatgctaga tatgaagaca gatatggctg gtgctgcgag
     2401 tgttataggt gctatgtgtg ctatatctca aagtaaatta aagaaaaatg ttatagcagt
     2461 agtggctgca tgtgagaatg ctttatcagg aggttcatac aaacctggag atataattag
     2521 ttctatggca aagaaaacta ttgaagtttt aaatacggat gcagaaggta gacttacatt
     2581 agctgatgca atatactaca ttataaacaa tgaaaaagta acaaaggtgg ttgatgtagc
     2641 tacattaact ggtgctgctt taacattgtt aggtaatgtt gcaactccta tagtaacaaa
     2701 taatgatgat ttttattgtg aattagaaaa agctgccact ttatcagggg aaagggtatg
     2761 gaagatgcca atttatgatg aatttaaaga tatgataaaa ggagaagaag cagaccttaa
     2821 aaacactggt ggtaaaaatg ctggatgtat aacagcagga gcttttattg gtgaatttgt
     2881 tgggaatact ccttggatac atatggatat agcggggaca agtacatcat caaaatcaat
     2941 tggatacaaa gcaaaggggg ctacaggaga acctgttaga acattgtact atttggcaga
     3001 aatctaaaaa acaattataa aatataattt ataaagccac gtggatattt ttgtaattta
     3061 agattatcta tgtggcttta ttttttattt aaataaatta tttactcttt ttcttttttt
     3121 caagtataaa agtatctcta ttaaataaaa gagcaggtat gtacataata ctagtaacaa
     3181 aaataaacgt aaaaatagca gttgtgctat gacttaaaaa accataatag tacctagagt
     3241 tggtgaatgt gtttagtaaa aatatgaatg aaaaataaat aaaacatata gctaagttat
     3301 ctctaagtgc atatctgtta actctaaatt ttctaacatc aaccatatat atatctgcat
     3361 aaattatcaa gcacttagag aagataaaga taaaatatat taagctagga aattcatagc
     3421 ccaagttttt agaaaaaatg agttctccaa ctgaacaaac taaactccaa gaaaaaaata
     3481 tattaaaaag atgatactgc ttaaaaatta gtatggctat acacagatat atacaaatat
     3541 aattgattcc aattggtaaa gagtttagag tactataatt acctgaaga
//
LOCUS       DACULQ010000253         3531 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.255, whole genome shotgun sequence.
ACCESSION   DACULQ010000253 DACULQ010000000
VERSION     DACULQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.255"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            136..1512
                     /locus_tag="JA154_17565"
     CDS             136..1512
                     /locus_tag="JA154_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAT7997635.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1799..2470
                     /locus_tag="JA154_17570"
     CDS             1799..2470
                     /locus_tag="JA154_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAT7997636.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2659..3456
                     /locus_tag="JA154_17575"
     CDS             2659..3456
                     /locus_tag="JA154_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAT7997637.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDVNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1326 a          402 c          663 g         1140 t
ORIGIN      
        1 ttataaaaat tactatcatt gtctttatac aatatttata ctataataga ataataattt
       61 agaggaaagc tctaaaaata aacaaattaa gggtcttaac agtagtgatt ttatgttaag
      121 gaggtataaa gaaagttgaa aacaatagtt agaagactta ttctctacgc agatggaatg
      181 cgtcctactc aaataatggt atctgggttt gctgcgatta ttgtaatcgg agcattgttg
      241 ttaacactcc cgatagcgtc gcagagtgga gagagtatag gcttattaaa tgctctattt
      301 acggctactt cagcggtttg tgttactggt ctagttatgg tagatacagc aacttattgg
      361 agtttatttg gacagatagt tataataaca ttgatacaaa ttggtggtct tggatttatg
      421 accgtagcca caatgttttc acttatggca cgaaaaaaaa tacaactaag agaaagatta
      481 ttaatacaag agtcattaaa tcaagctgat ttatcgggac ttgtaagact tacaagattt
      541 gtattaataa taaccataac aatagaggga ataggtgcac tggttttatc aactgtcttt
      601 attccacaat ttggtttgtc taaagggata tggtatagtg tatttcacgc aatatcagcg
      661 ttttgtaatg ccggatttga cttgatgggc agtgtaagtg gcccatttac atcgttaaat
      721 tcttatgtaa acaactttac agtatcaatg actgtttgcg cacttatagt gcttggaggg
      781 ctaggattcc ctgttgtact tgatattgta agaaaaagaa gattttcaaa attaaatgtg
      841 cattctaagg ttgtattgtt ttcaactgct acacttattt ttgtgggtgc attatttatt
      901 tttcttatag aatttaatca aaaagctact atggcggact tgcctttaaa gggaaaagta
      961 ctatcagcaa tatttcaatc agtaacagca agaactgctg gttttaatac gcttgattta
     1021 gctactttac gagaaagtag tgtatttgta atgataattt tgatgtttat tggagcatca
     1081 cctgcatcaa ctggtggggg tataaaaaca acaacactag ctgttttaat cattacagtt
     1141 agaagttttc tatcaggaaa atcagatata gaagcttttg aaagaagatt agctccatca
     1201 actataaaaa aatctcttgg tatatttgta atcagtatat cagcagttat ttttggaaca
     1261 ttgattattt caataactca gcctaatttt acattagttc aatcagcttt tgaggttaca
     1321 tcagcacttg ctacagttgg ttcaagttta gctggaactc caaacttaaa tgctttaggt
     1381 aagattataa taataatctt tatgtttatg ggtagagttg gttctcttac tttatttatg
     1441 gctatacttt ctggtggtag aagaaagagt caaccaatca gatatgcaga aggtaaaatt
     1501 atggttggtt aagttaccaa ggagtaatat aaatttataa tggatttaga gtagcactgt
     1561 aaatctgttg ataaaataaa tgagcctaga tgtaattata tgaagaaata aaatcgacta
     1621 acgaacaaaa ttaattaatg aaataaaaaa acagggaaat aaaatacaaa taaaaaggta
     1681 aatcaaaatt aaattaatct gtgtatataa aattatatat tgtgtataag ttttgtatgt
     1741 gtaaacaatt aatttaagag atattatata aattgtgtat tagggaggaa gtttcgagat
     1801 gaagcaatat atagtcattg ggtgtgggag atttggaagt tcagttgcgt ctactatgca
     1861 tcttttagga catcaagtaa tggcaataga caaaaatgaa gattcagttc aaagtatatc
     1921 tgacaaggta acccattcac ttatagtgga tgttactgat gagcaagcgt taaggtcatt
     1981 aggtttaggt aactttgatg tagcagtagt tgcaataggt tctgatataa gggcatctat
     2041 aatggcgact cttatagcca aagaaatggg tgtagagttg ataatatgta aggcaaagga
     2101 tgaattacaa gctaaagtgc tttataaaat tggcgcagat agagttgtat ttccagaaag
     2161 agatatggga gtaagagttg cacacaattt agtttcagat aatatattag accatattga
     2221 acttgaccca gagtattcaa ttgttgaaat cgtaactcca aatagttggg ttggcaagac
     2281 acttatagag cttgaattaa gagctagata tgagataact gtacttgcta taaaaacagg
     2341 taaaaatata aatgttacac cttctccaga tgaggaactt acagccggaa gtatcctagt
     2401 tataatcggt caaaatacta gtataacagc gataacatct ggaaataagg ggataattag
     2461 aagaagataa tttactattt aatatatatt taattgcaat gaaagtagat agtatcatat
     2521 aattatgaat agttatatga tactattttt ttaatcgaag gtagtattta tttgtaagat
     2581 tagataaaga gaaattaaat taaaagtaag gaggctgtgc taatataaaa atttataagt
     2641 tattagcatt tagataaaat gattacaaat ataaacagta aggataatga aaagttaaag
     2701 tatacaagag cactattaaa atcaaagaat aggaataaag agtcaaagtt cataatagag
     2761 ggatacagaa tagtaatgct tgcacttgaa tgtatggcaa accttgatta tgtatttatc
     2821 aatgaagaat ttgaaaataa gaaagaacat gtaaaactat tagaagattt ggataaaaaa
     2881 aacacaaaga tatacaagac tactaataaa aactttaaag aattagtgga tacagaaaat
     2941 actcaaggaa taataggtgt agtttcattt aagaaaaaaa aattaagtga aagtataaat
     3001 aaaaaagata aatttgtatt gattttagac agaatacaag acccaggaaa tatggggact
     3061 ataataagga ctgctgattc tgctggagta gatgccataa tagcactaaa gggatgtgtc
     3121 gatatataca acccaaaagt aattaggtct actatgggtt ctatttttga tgtgaatata
     3181 attgatgctt cacaagatga aactgtggac atgcttaaat cattggattt taatatagtt
     3241 tcaagttact taaatacaga aaatttttat gacaaaatag attatggctc aaaagtagca
     3301 ttggtgatag gaaacgaagc aaatggaata aatgaagaac ttgtatcaaa gtctgatatt
     3361 ttggttaaga tacctatata tggtaaagcc gagtcgttaa atgctgcgat aagctctgct
     3421 atactgatgt atgaaataaa aaaatactta atttaatgta ttgtaataaa tataatgtca
     3481 tgttataatc ttgaattaaa tagtagagta tcaaaaatag tcaatatata t
//
LOCUS       DACULQ010000254         3517 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.256, whole genome shotgun sequence.
ACCESSION   DACULQ010000254 DACULQ010000000
VERSION     DACULQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.256"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1759)
                     /locus_tag="JA154_17580"
     CDS             complement(<1..1759)
                     /locus_tag="JA154_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT7997638.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GVSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEK"
     gene            complement(1971..2540)
                     /locus_tag="JA154_17585"
     CDS             complement(1971..2540)
                     /locus_tag="JA154_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7997639.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(2724..3359)
                     /locus_tag="JA154_17590"
     CDS             complement(2724..3359)
                     /locus_tag="JA154_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089752.1"
                     /note="binds to single stranded DNA and may facilitate the
                     binding and interaction of other proteins to DNA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAT7997640.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         1071 a          629 c          331 g         1486 t
ORIGIN      
        1 atttttcaat ttttctatat aatgtagctc ttgatattcc aagtgcttta gctgttaatt
       61 ctttatcttt tttataatct ttgtacttat ttaaagcttt tattatctca atcttctcta
      121 gttcatcaag tggaattatc ctatcgcttt taacttctaa ctcttctaca gaggtattct
      181 taattttttg aggaattaca ttcattgtaa ttattgaaga agaagacata ttcacactat
      241 attcaataat attctgcaac tctcttatat tccctggcca cttatattcc atcatagcat
      301 caacaaccct gctatctatg tcaataacat ttttatctaa tttttttgaa tactctttaa
      361 ccatatgctt gattataagt gggatatctt cttttctctc tcttaaactt ggtaatttta
      421 taggtataac atttaatcta tagtataaat cttctctaaa agtgccattt tgaaccattt
      481 tttctaaatc tttatttgta gcagcaataa ttcttacatc tattgaaaca tttgatttac
      541 ttccaatttt attaagttcc ttttcttgta atactctcaa taactttgac tgtaaatgca
      601 aactcatatc tcctatttca tctaaaaata tacttccctt atgagctact tcaaacattc
      661 caagctttcc acctttttta gctccagtaa aagctccctc ttcatatcca aataattcac
      721 tttctaataa attgtctggt atagcagcac agtttacagc tataaaagga ttatctactc
      781 tattgctatg gttgtgtatg gctcttgcaa atagctcctt acccgttcca ctttccccag
      841 taataagtac tgttgatgtt gatttagaag ctattagggc ttccttttta gaatttctta
      901 tagctacact ttctccaatt atgttttcca attttattct ataatcttta ttcatcttat
      961 tgtagttttt tatagcatct cttttatcta taaaatcaat aacatatccc tttaactcat
     1021 tttttaatac tattttattg atgttgtata ttcccttcga atcatatccg tatcttttat
     1081 aaaaaaaact acaggatttg tctttcttaa agttatttat agaaggtttc tttataaaat
     1141 ctaattcgac aaatatgttt ttgccagtta tactatcctt tatattgaaa agctctttaa
     1201 attttgaatt atatttatca atttttccat ccacatcaat agacacaaca gctttgtcca
     1261 tattgtttaa cagtgtctct aattttttct tttctaattc aagctcataa gttttaattt
     1321 gagcttttaa tttattagat attaagtctg ccattttgcc taaaaagttt ataagaccac
     1381 ttttattatt ttctataatc tgtgcttgtt cctttgtaaa ggctataagg cctataacac
     1441 catgagagac tccatcacat ataatgggac aacacacctc tgcaaactct acacaagccc
     1501 cacttttata gcattcccta catatatcac tactctttgg gtcatcaatt agtattgtaa
     1561 tctgctctgt aagtgatttt ttaaaagccg tatatttatc tactttctca cctattttgt
     1621 ctatatatac tcctgtacct gcaatcctaa ataactcctt atttactata gttacatcta
     1681 tattgagtac tgatactata gcttcagcta tattttgaat atcagatttt atatattcta
     1741 aacctatcat aatcatcatt ccttattttt ctttaacata agtatttgtc ttatttcttt
     1801 aagaatcaag tatatcttat ttaaagtaaa ttggattgac atcaggatta caaagagcat
     1861 aacaattatt attaactcat aatttatttc catctaaaca tctccttata acaaaagact
     1921 aggcttaacc tagtctttta tattatatcc atatttatat tttaactcat ttatcttctt
     1981 tttcccacat aaatatattt taatttattt gactctttaa tcataccatc aaagtttaat
     2041 cttgaaaata tattaaatac ttcatgtgga tagtgaatct ttgcaacaga accacaaaat
     2101 ttatgtttaa gatagtttaa cttaactttt aaattcttta aatttaaatc ctctaatata
     2161 acctctccat tgccctttaa cactctttgt atttcagatg aagctttttc acaatctgta
     2221 ataacattaa attggtcaac tattattatc ttgttgaact gattatcatc aaatggtaga
     2281 aactctaact tacctttaac tacattgcaa ttttcattat acactagaga ataacagaca
     2341 tcttcataat tactattctc cagtatagtt acagaagttc caagacctct caatctcttg
     2401 cctaggtttc ctatatgacc aacaagcaat attttatcac ctacattata ctcaattgta
     2461 tccaaaaaac attctaattc atcagatgta tatttagctt taaccggctg tttatttatc
     2521 tgacataata tgcttttcac aatgcttcac ctcttcattt cctcaattat aaattatgta
     2581 cagattacat tataataaat ggcgagcaac tattcaagag atatttcatt agtatttttt
     2641 taaacaatct ttatacattt taatatgata ttataccaaa tttattagga ttttattcat
     2701 aaaaatcagt ctatttttta tgtttattca ttgttatttt catccaattt ttttattttc
     2761 gatatggata cttcatatgc catttttttg accacattat tttcatctat ttttttctca
     2821 tattctctac tttgaaccct accccaaagc tgtattctat caccaacctt tagattcttt
     2881 gcaaatttag catttctacc ccaaactatt gatggtatat aatcagattt attataaggt
     2941 ctgtttatag caactaataa atcggttatt tcccttccta aaggtgtctt cctatacaca
     3001 ggttccttac atacatatcc atctaaaaat atactattag ggtctttatt ttcctcgtct
     3061 atgcttttta tttctctgac aaacactgta agcactaatc tatttttatt atttaatgtt
     3121 ttgttatatg atcttaattg gcctacgaca ttgactaaat tttgtttatc aaggtcaata
     3181 tcttggagaa gtctttctga tacagtcatt ggaagtatat caaaagaatc gcttagtctg
     3241 tttattttta ttttcatatt ataaaatttt tctccaaata tctcatggct aaattctagc
     3301 ttattgtcta actcccctct taagtttact acgttattct catctttaac agctatcata
     3361 ttttatccct ccacaattgt taattcttat atttctggta tcagcttata taataaatat
     3421 atttataagg gatgtatttt attacaaatc tttgattgct gtttaaaagt ttttatttag
     3481 ttataaattt catttaatat ttcactttta taattat
//
LOCUS       DACULQ010000255         3514 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.257, whole genome shotgun sequence.
ACCESSION   DACULQ010000255 DACULQ010000000
VERSION     DACULQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.257"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            118..732
                     /locus_tag="JA154_17595"
     CDS             118..732
                     /locus_tag="JA154_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAT7997641.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTNRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            750..1112
                     /locus_tag="JA154_17600"
     CDS             750..1112
                     /locus_tag="JA154_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997642.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1218..1715
                     /locus_tag="JA154_17605"
     CDS             1218..1715
                     /locus_tag="JA154_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HAT7997643.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
     gene            complement(2106..2240)
                     /locus_tag="JA154_17610"
     CDS             complement(2106..2240)
                     /locus_tag="JA154_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997644.1"
                     /translation="MSYPSALSAKVYPLPLPITCSVLPSRGIVVEVSPIWSLPADCPF
                     "
     gene            2519..>3514
                     /locus_tag="JA154_17615"
     CDS             2519..>3514
                     /locus_tag="JA154_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT7997645.1"
                     /translation="MIAVKKAYKFRIYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKVRDKQVPKGRILNATISKEPSGR
                     YYVSLCCTDVDIEAFENTNNHIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IKNHKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEV"
BASE COUNT         1411 a          398 c          589 g         1116 t
ORIGIN      
        1 agtactaaag acttgcaaac gttttttgac aagtctttat tttgtctaaa ttaaacaaac
       61 ttttttacaa ataaatacat aaaatttaaa ctaatctatt tattggagga gataaaattg
      121 gaagaagtaa ttgcaaaaat tattactata gaaaatggat tcaaagaaat tgaaaacatt
      181 gctaaaaaaa ttgtaaaaaa taatgactca aaaaagtgtt attatctatc aatctactta
      241 tataggtcag aatattatca agtaagagaa ttggctgtgt ttatacttgg ttttatatca
      301 gtaactattt ctgaagcttt gttattttta aaagataatg tgagtaaaga tgaaaactgg
      361 agagtacaag aaatactagc taaatcattt gattattatt gcagtgaagt aggatatgaa
      421 aaggcacttc ctgttataaa agaatggctt aattctgata gtccaaacat aaaaagggct
      481 gtgacagaag gtcttcgaat ttggactaat agagactatt ttaaatctaa tcctgacgtt
      541 gctatatcac tactttctag tttaaaagaa gatgatagcg aatacttgag aaagtctgta
      601 ggtaatgctc ttagagatat aagtaaaaaa tatccagatt tagtaaaaaa agaattagat
      661 tcatgggata tatcaaataa aaaagttgag tttgtatata agcttgcttc aaaatgtatc
      721 ttggcaaagt aaaatggagg tgaacttatt tgaaacttcg tgatgagtat acttgcccat
      781 tagaactaac acatgatatg ataaaaggca aatggaaatc tattatatta tggagattac
      841 gcataggacc aacttcctta tcaaaacttg aaaaggaaat caatggtatt aatcaaaaga
      901 tgttacttga acaacttaaa gaattaattg aatttggtct tgtagataaa aagacatatg
      961 aaggttaccc acttaaagtc gaatactcac ttacacctat tcgagggaaa caaatattgg
     1021 atgctttggc tataatgcaa gatataggaa ttagctttat gttggataat ggtatggaag
     1081 atattcttaa ggaaaaaggt gttttaaaat aaatagatac ccacaaaaaa gtaagtaatt
     1141 taccattatt ttcaactggt atataattaa attatagata tattctatta atttattata
     1201 ataggaggta aattattatg aaagtaacta gtacaggtat agtaaatggt gttatagaag
     1261 ataaatatgg taaaagagga actcagttca atgaaggtgg tatgcctaca tattcactac
     1321 ctctaaagtt tgaagatgct ccaaagaata cagtatcctt tgctgtattt ttagaagata
     1381 aagactctgt tcctgtatgt gggtttgcat ggatacattg gttagctgta aatataacaa
     1441 aagatgagtt aaatgaaaat gaaagtgtaa cagctactga ttttgtacaa gggactacta
     1501 gttggcatgg taaactagga ggtcttgaca gattagaggc cagtgtatat ggtggaatgg
     1561 ctcctccaga cagacctcat gtctatgaaa tacatgtatt tgcacttgat actatccttg
     1621 accttgaaaa aggattctat atgaatgaat ttttcaaagc tattgaaggg catgttttag
     1681 atacatttac actaaaagga agttattcta attaattagg ttgactatag ctaatcatta
     1741 attaaatgat acatttaatt gatgattagc ttaattgtta ttagaatatt ttctgcactt
     1801 tatcttaata tatattttag ttgactggta ttttaatttc tattagatta ttatcaattt
     1861 atataaattg attaaaaaat agctacgcac actcgtgact ttagtcatga gttagtagct
     1921 aaaatagtca gcatataggg aaacttatat gtagagatag gatagaacct atccaatact
     1981 acttgaattg ctaaaaaccc ctaaagctaa ccaaactaaa acataaggat gaaataaacc
     2041 taagtgtgaa agttacgaaa gtagaaaaaa ttggttagat ggtataaggt taaaccctaa
     2101 gtactttaaa atgggcaatc agcaggtaaa ctccaaatag gagaaacttc aacgactatt
     2161 cctcttgagg gaagtacact acaagttatc ggtagtggaa gtgggtagac cttagcagat
     2221 aatgccgaag gataagatat agtctgtgct tatgtgaaag tgtaagaagt tcataagaga
     2281 actgcatagg tagtagcgaa cttatgtgaa cgacaacctc aaaaatgata aaagttaaga
     2341 cttacggttc ttatatatgt tatttaaaat taaatataaa accttctaaa taattgtttt
     2401 aaataattac atttgttaac attattatag gtgattaata aattgttcac cacagcatta
     2461 tataatatat taaatattaa aatcattaaa aagtgtatca aaaaacaatt aaagtgaggt
     2521 gatagcagtg aaaaaagctt ataagtttag aatatatcct aataaaaaac agcaagagtt
     2581 aattaacaaa acttttggat gttgtaggtt tgtatataat aaatatcttg ctaaaagaat
     2641 agatgtttat aaaaacgata aagaaacttt tacttataaa cagtgtagtt ctgatttaac
     2701 caatttaaaa aaagaattaa attggcttaa agaacctgat aaattttcac tacaaaacgc
     2761 cttaaaagat ttagataatg catataaaaa attctttaaa gaaaaggcag gatttcctaa
     2821 atttaaatca aagaaaatta atagattttc atataaaact aactttacaa atggaaacat
     2881 tatgtattgt ggtcaacata taaaactacc taaacttggt atggttaagg taagggataa
     2941 acaagtacct aaagggagaa tacttaatgc tactatatct aaagaaccaa gtggtagata
     3001 ttatgtatca ttatgctgta ctgatgtaga tattgaagca tttgaaaata ctaataatca
     3061 tataggttta gatttaggaa ttaaagaatt ttgtatctca agttgtggag aatttataga
     3121 aaatcctaag tatcttaaga agtcattaaa caaacttgct aaattacaaa gagaattatc
     3181 gagaaaaaca atcggtagtt taaatagaaa taaagcaaga ttaaaagttg caagacttca
     3241 agaacatata gctaatcaaa gaaatgattt tctacaaaaa ctatctacta aattaataaa
     3301 agagaatgat attatttgca tagaagattt acaagtaaag aatatgatta aaaatcataa
     3361 actatctcgc ttaatttcag atgtatcatg gtcagaattt attcgtcaat tagaatacaa
     3421 ggctaattgg tatggtagac aaattgtaaa ggtaggtaaa ttctttgcaa gttcacaaat
     3481 atgcaataaa tgtgggtata aaaacgagga agtg
//
LOCUS       DACULQ010000256         3509 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.258, whole genome shotgun sequence.
ACCESSION   DACULQ010000256 DACULQ010000000
VERSION     DACULQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.258"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(427..648)
                     /locus_tag="JA154_17620"
     CDS             complement(427..648)
                     /locus_tag="JA154_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997646.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            1073..1213
                     /locus_tag="JA154_17625"
     CDS             1073..1213
                     /locus_tag="JA154_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997647.1"
                     /translation="MRNFIKVNTKINFKAKKFFIRFNKTNKYSLVNNVLNKNFVKSIK
                     IS"
     gene            1228..1485
                     /locus_tag="JA154_17630"
     CDS             1228..1485
                     /locus_tag="JA154_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997648.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            1954..3192
                     /locus_tag="JA154_17635"
     CDS             1954..3192
                     /locus_tag="JA154_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997649.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDKNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1434 a          308 c          524 g         1243 t
ORIGIN      
        1 tatttcaatt gtcaatatta atgttttgct tgttagtatt agtatttcaa ttgtcaatat
       61 tagtgttttg cttgttagta ttaatatttc aattgtcaat attagtgttt tgcttgttag
      121 tatcagtgtt tcaattgtca atattagtat tttaattgtt gactctagtt attgctgata
      181 tagaaaatat ttaatttatt tcaattaatt agattaaagt agggtagcat ctaaaatagc
      241 taacatgttt aagttgaaat acgtttaata tattaatttt tcaaagcatt aatatattaa
      301 agttataatc aaaattgaag ttataatctg ccaaatctat agcgtattaa gtgcgttata
      361 taacttaatc ttttatataa agagttaatt tttttaattt gtaaagcaga gtacgtttaa
      421 aaggtactat ttagataatg atgtactatc taattttaaa atatctacaa tagggttctc
      481 ttgaagactt gctattgctt ctgcaacagc ataaacatct tcatttaatg cctctgcttt
      541 tacatttgca aatgatttac ttttaacaac tgccttaccc ttatcatcaa aaccagcatc
      601 caattttagt tttatactag ataaattttt tgttgaaaca acttccataa aattacctcc
      661 taaaaattta atttcaaaga tttgtcgact ttctttgtta gctataatac tatcatctaa
      721 gataatatcg gagttaaaca gtaatttcta aagatttttt gtgtaaattt ttataggaaa
      781 aattttactt ttttgctgat gaaaaagttt acaagttatt tgtgaagata tgttcttagt
      841 gttttgaaat agtatttgta tatattcatg cttagttgag atagttttgt atggattttc
      901 atatgtgtag agttaaatgt taggtactaa agtttatgtt aaattaaatg ttaaagctta
      961 tgttaaaaat tttattaaat ttaatctgta aataaaaaag ttaaaagtcc aatattaaca
     1021 ttttactttt aaaccattaa aatctttatt aaagctaaca aagttataag ttatgagaaa
     1081 ttttattaaa gttaatacaa aaattaattt taaagctaag aaatttttta ttagattcaa
     1141 taaaactaat aaatatagtt tagtcaataa tgtattaaat aaaaactttg ttaagtctat
     1201 aaaaatatct taaaggaggt gtaacttatg aagataaaag tggaggatat accagttcaa
     1261 tttcatgcaa tggtagaaat aattggaatt gataagttta tagaggttgc caagttgtat
     1321 ggaggaacaa atacttatat acctacgtat aaggggcttt ttagatatgc tagaaatagg
     1381 gatatagtaa aacaatttaa tggagtcaac cacaatgaat tggctattag atataatatg
     1441 tgtgtaagca atattaagcg aatactcaat gaaaataatg tttaaatata ataaaataat
     1501 gtttaaacat aatgaaaata gccttgttaa acgtaagagt aaagacaaag aagatgtttt
     1561 ataaagtttt tttagacaaa ttaactaaat ttactactta ttaatttaaa tacattgtat
     1621 attctataaa aaaatttgtt aaatactgga gatatattga gaatacaaag aaaatggtcg
     1681 aaaatatttg ttggctaaaa aatagataaa atgatacaat tgcttgtata aaggggaaaa
     1741 ataaataaat aaactatata aataaagaaa tagcaattaa tatgacataa tatatttata
     1801 aaattaagaa tataaataga tattttaagg tattagttgt taaattagga aatattaaaa
     1861 aaaatatata tttatattta aactattttg ttatttatat aaaaatactt atctagttac
     1921 tagcaattat tagtagaaat gggggaaata gagatgaata agggatttta caaattaaat
     1981 tcagtggtat tgaactttga tattaagaat gatgttaaca agataagatt taatcattat
     2041 ataatgagaa aagaaaacat acaaagactt caaggaactc tgccaaacgg acaattttat
     2101 atgacagtga gaagagctgc tacagattta caactttcta tttctactat aagtaggctt
     2161 gtaaaagagt ttttggattt agggataata agattagtgt ctaagggtgt aaaaggtaat
     2221 cgttctgtat atagttacgt aagtatagag tttaatggaa gtttatttga tgatagcaaa
     2281 tatatggaac ttgactgtgt atgtaatgat gaatttattg ataatagtgg catagagttt
     2341 gattgtgata gcaaagataa ttcttttaat ggagatataa atgtagaaac tgtgcattgc
     2401 attaatgagt atttgtctga tgatgatgta gagcataatt ataacaattt ccataataaa
     2461 gatactataa aggataaagc catttctaat attagcacta tcaaaaaata tattgatgaa
     2521 tcttttgata aaaataaatc agaaaattct aacttacaac aggagtcaat aaatttcaac
     2581 tttaatgata catgcaatga aatagataaa agaacgaata atagtataga aaataatcaa
     2641 aataaaaggc ctatgagatt gagtgacatc agtcttttaa gtgataaagg tatgacaaat
     2701 aaaaagaatt actttaatca taatcatggt atacaaaaag ggactaaatc tgtaacaaag
     2761 aaaaaagaat ccttaaataa atttaataaa aaaaatatac agcaaagcat tataaatatg
     2821 ctaaataaaa aatcaggtaa gaattttaaa atcgattctc caataagcat aaaacttatc
     2881 aatgaaaggc taaaggaagg ctatgtctta gaagatttct acaaagttat tgaggtaaaa
     2941 gtggctaagt ggaaaaatac cataatggaa atgtatataa gaccagaaac tttgtttagc
     3001 tataagtttg aatcctatgt aaatgaaaac atatctatga aaagtcaaag taatgttcac
     3061 ccatgtaaag aaggcaatag taatgatagc agtaattata tacattctga ttatacaaca
     3121 tatgatggca gtgtgcgtga gagaaaaaaa tatgagaatg taagatactg gaatcctata
     3181 tgtgaaattt aattagttta agaatgtatt tatgataaat aagatattaa tagagaatag
     3241 tgagctatag caaatgatat atcaatagtt ggtagtaagt tatattaagc aagatattac
     3301 tatcaggtag tgaattatat tgaacgatat atcaataatt ggtagtaaat tatattaagt
     3361 aagatattac tatcaggtag tgaattatat tgaacgatat attaataatt ggcagtaaat
     3421 tatattaaat aagatattaa tatcgagtaa tgaattatat tgaacgatat atcaataatt
     3481 ggcagtaagt tatatcaaat aagatatta
//
LOCUS       DACULQ010000257         3495 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.259, whole genome shotgun sequence.
ACCESSION   DACULQ010000257 DACULQ010000000
VERSION     DACULQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.259"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            541..1293
                     /locus_tag="JA154_17640"
     CDS             541..1293
                     /locus_tag="JA154_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997650.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            1283..3394
                     /locus_tag="JA154_17645"
     CDS             1283..3394
                     /locus_tag="JA154_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997651.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         1326 a          491 c          472 g         1206 t
ORIGIN      
        1 tctaaaatta tctatcatac ctatactaat aatacttttt aaccttttta ttatacattt
       61 caattgtttc taacaaccta tttatttcca aaactccaat atattttctc aattacattg
      121 tgttaatagc ccctaaatcc tttcaaatta tttacaatac attctattaa ataatcttac
      181 tgctctataa ttcttttttt ctttttaacc ctactataca ttatttacat catttttttc
      241 aatacaacat tcgttattct ataacctttt tacttttact atatacttct tttctaacta
      301 caccttcaat gttccacatc atattacttt agcacaacaa ttaataccta aaaataaccc
      361 tctaaatcct ccaataatat aatcaattta actataataa taacctctca cactatccaa
      421 acaatatatt ttcaaacatt acaaaaaaac atccaactat tacaatatag ctcatatccc
      481 tcctcccctt tcaaatgcta tcattgactt acaagataaa tgaaagtgag gatgatatat
      541 atgcttaaag taaaccaatt atgtaaatca tataaaacag gcagtaaaac atatcctgta
      601 ttaaaaaata tttcttttga aatcaacaaa ggagaatttg tcgcaatcat gggttcatca
      661 ggaagtggaa aaactactct tttaaattgt atttcatgtt ttatcccaat tgataatggg
      721 gaaataattc ttggaaataa taatataaga aatttaaaag aaaaggaact ttcagaagtt
      781 agaaatcaac aactaggatt tgtattccaa gacttcatgc tatttgatgg tcttagtgtc
      841 tttgaaaata tatgtgttcc acaaattatc aaaggtagtc aagtcttaca aatggaaaac
      901 cgtgcaatta atctatgtga gctatttggt attgaacata tcagaaaaaa ataccctgcc
      961 gaaatatctg gtggagaaaa acagcgtaca gcagtagcaa gagcactcat gaacaaccca
     1021 gatttaatat tagcagatga accaactggt aaccttgata gtaagtcttg tcgtacagtc
     1081 attgaatcat ttttaaaagc aaagcaagaa ttagatgcta ctatactcat ggttacacac
     1141 gatagctttg cagcaagctt ctgtgaccga gttattgtgc ttaaagatgg aataatttat
     1201 aaagaagttg taaaatcaaa ttcaagacat aaattcctag atgaattact agaagtttta
     1261 aaagaaatgg gaggcgctca agatgacaat taataaatta tacagtaaac ttcgagccta
     1321 taattggaag aactatacaa ctttattctt ttgtataata ctttctgtgg tattagtaac
     1381 ttcatatgct ctaatttatt ttagcccaac aatacaaaac atacttccaa ctggtggaga
     1441 tagcagaaaa caggctacaa tgatattctc agttgctata ttaggatgcg ctgtctttac
     1501 tacatacgct tcttccctct ttttaaaatt taaaagcaag gaaattggta ttttcatggc
     1561 tctaggaaca gataagaaga aattaaaaaa acttctattt tcagagatat tgttaatttc
     1621 aatagtatgc tgttttttag gtcttatatt aagtattcct gtatcttttg gtatatggaa
     1681 aatatttcaa ttagtgatta ttgataccag agaaatgaca tatcattttg gaactcaagg
     1741 attcatatat ggatttatat ttagtgtatt tgtgacatta tgcatattta tactttctgt
     1801 aaaatttatt aatcgtacaa atataattga tatattaaat gaccagagaa tatctgaacc
     1861 aatacaggat gtaaaggact ggtatggtat attaggtata atttttatta tactcggtat
     1921 ttttctagga tatctcgtac cttcactaac tataaacaaa ttaaattata aactaccatc
     1981 tatatggagc tttacttatc tactctctct tattggaata tatatgatta tgacatacat
     2041 agttgtacat gctaaaagag gaaaacatcc agagaaatac tataaaaaca tcatatcaac
     2101 tagcatgatg agatttatgg gaagacaaac agtaaaaaac atgtgtgtta tttcattttt
     2161 gattgcagga gcattatttg ctgtattcta tgttcctaat atggttacag gtatctatga
     2221 tactcttaaa aataacccta tagattatac ttttacctac aatcaaagag ataaacaggt
     2281 atcaaaaaaa gatattgaac aattagcaga taaacacaat ataaaaattc aaaactatat
     2341 ggaaattcct tctatagaat taattgttga tggtgtagaa tcaatatatc ataaagatgg
     2401 aactatcgaa aaggtatata tgaaaaaaag ctcctatgat atgttcttta attctaaaga
     2461 tttttccaag ctaattggca aaccaataac tataaaaaat ggtgaatatc ttgctgttgt
     2521 aaataaagga aatgaaaatc aagattataa atataccaaa tttatatcaa gcccagtcac
     2581 agaaaaagaa atccccttta gatatgctgg aaaaataata tttaatggtt ctttttttca
     2641 aaatagaagt atgaacgcct atgtccttaa tgatgaggat tataaatcat ttactaaaga
     2701 tttatccata aaaaattaca ataattctat actatttaat gtagataaag atacctatgc
     2761 ttttgccaat gacttaaaga aagaaattat aaaaagaaca tctaaggaaa tgggcatgtt
     2821 cagtggttat gatgaatacg taaaaaaacg ttatgaatca actggaagaa aatattttat
     2881 ggatgaagaa tatccaagtg gtgaaggtca tttaaaacta gacccaaaca acaatcagtt
     2941 gtatcttaat tggaaatact atcctaactt taaaatttta caaagccaag atatgataaa
     3001 aaacatggca gtcttcttaa tgttattcat atatgtggca atcatatgtt ttacagcagt
     3061 aggaatcatt gcatatacaa ggggtgttac tatagctata aatcacaagc aggtctttat
     3121 ggatttaaat cgattaggag caaacaatga ttatataaag ttctgtatta gaaatcaatt
     3181 aaagaaagta ttttcatacc ctcttattgc aggaagtttt ttaatttatc tgtttaattt
     3241 tatgattatg ttaaataatg atggtagaat cgtcaatagt gaggtcaatg ctcttttgat
     3301 aaacttaggt tttataggga tttgtggggt ttatatgtac cttatatatc tattaactta
     3361 taagaaattc aaaaaaatta ttggtatttt ataataaaaa aggttattag atttttaaag
     3421 taataaaatc attggtgttt tataataaaa aaggttatta gatttttaaa gtaataaaat
     3481 cattggtgtt ttata
//
LOCUS       DACULQ010000258         3395 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.260, whole genome shotgun sequence.
ACCESSION   DACULQ010000258 DACULQ010000000
VERSION     DACULQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.260"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     repeat_region   17..707
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=84..112
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(1366..>3395)
                     /locus_tag="JA154_17650"
     CDS             complement(1366..>3395)
                     /locus_tag="JA154_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT7997652.1"
                     /translation="HTNEIIFMNKKMKEEYNILDPEGKVCWQVLYPEKNSTCSFCKVL
                     ELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQDGTVVHMHQSIDIANSTSLNKPI
                     KINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDALIRGTDEYIYICNMKTGVFRYSP
                     SQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSNTEIGKNQMDYHTVEFRAKNRSG
                     EYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKIDSLTQLLNYHEFMSVFEDKISNP
                     MIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQSIQSILPDNAELYKLDGDEMGI
                     LVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITISAGCVIYPKHGDTVKELYKCAS
                     YSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLKASINDDFRGFSLRFQPQVDTES
                     HKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMINIVGAWVLRMALRTFRKWIDYYP
                     FFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNLVLELTESHTVQNMSILQFKFKA
                     LQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRVFVKDILKSKFDATFIHFIVAIC
                     HDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGKPQTATEIFDLLKLDN"
BASE COUNT         1182 a          547 c          330 g         1336 t
ORIGIN      
        1 aaatatcaga attgctattt atattccata tagttaatat aaaactataa agtgttactt
       61 ttatttattt tattttttac ataatttaca ttccatatag ttaatataaa acgcattata
      121 tggctgtgtc attttgcatt ctattacata tttacattcc atatagttaa tataaaacct
      181 gcttaatgca gaattaagag aggaagtgtt ataagattta cattccatat agttaatata
      241 aaactggttg ttcctgctcc ggtgttggtg gaactggtac atttacattc catatagtta
      301 atataaaacg aatatgtatg gataattttt tacttaacat attagcattt acattccata
      361 tagttaatat aaaacctgct gtaatttctg atgaagcttc tccactacca ccatttacat
      421 tccatatagt taatataaaa cccttcttta actcttatcg tatttaaacc tcttataaca
      481 tttacattcc atatagttaa tataaaactt tttgtgttaa gaagaaaaaa gaaattttga
      541 taatatttac attccatata gttaatataa aaccttaaag aaaaaatgga ggaagattaa
      601 gtgaaaaaat tatttacatt ccatatagtt aatataaaac tggatttaaa taatcaaaac
      661 aactccggtt taaaaaacat ttacattcca tatagttaat ataaaacccc aaaataaact
      721 tagtattttc aacacttaca cgtatacaac tctcttaaat ttgcagtgaa cgagcaatag
      781 tgcaattgat aacgcttatc atacactctc aacgccttat atttcaactg ttacgccaaa
      841 ttttttcaca aatatcgctc actgcaaaat tcctataatt ttattatatc ataaatatat
      901 tacttttgaa tattgatacc aatttatacc ataataaaag caaaaaaacg taatctatct
      961 acattagatt taaattctat aacttaaagt tttttacata atatttatgc ttgtttaatc
     1021 aatctaatat atcattcttg tatattgctt ttaatagtct tttttcttaa ataaatatct
     1081 atctgaacaa gcatagttta attaaaaact tttgtattcc cttaaaactg tatctacctc
     1141 ttattatttt atttatgata tattattttt aatctaaaaa tagatatcta tttttattat
     1201 ggttatccct ttttaaaata ctttaatcat atactattcc caatttaaca tccattgaaa
     1261 tgctattttc ttatattgca atttataatt gatatatctt caatataata atttaatgta
     1321 tttttgattt taaagctttt cagtgatatt attgataaat tagaattaat tatctagctt
     1381 taaaaggtca aagatttctg ttgctgtctg cggttttcca aataaatatc cttgtatgta
     1441 atctggcttt atttgtttaa ctaaatcata ctcttcttgt gtttctacac cttctaaaca
     1501 tacttttatt cctacatcat gacaaattgc aacaataaaa tgtataaatg tagcatcaaa
     1561 cttactcttt aatatatctt taacaaatac tctatcaatt ttcactatat ctattggaga
     1621 aaattttaaa acttctagag acgagtatcc agtaccaaaa tcatccatag caatataaat
     1681 tcctaaatct tgtaaagcct taaatttaaa ctgtaaaata ctcatatttt gtactgtatg
     1741 actttctgtc aattctaaaa caagattttg ataaggaaaa ttttcatcat ctataatttt
     1801 tactatatct tcaataaatg tgtcttctaa tatttgaaca gctgaaacgt ttacactcac
     1861 tttaaaaaaa ggatagtaat caatccattt cctaaaagta cgtaatgcca ttcgaagcac
     1921 ccaagcccct acaatattta tcatatcatt ttcttctaat attggaataa attccaaagg
     1981 agaaattgct ttgcatttgt cattagtcca ccttaaaaga acttctacac ctattatttt
     2041 atgactttct gtatctactt gtggctggaa tcgaagacta aaccccctaa aatcatcatt
     2101 aattgatgcc tttaaatccc gcatcatttc taatgaatac attttatttt ttaatatttc
     2161 ttgggagaaa aaaacaagcc tgtttttacc atgctctttc gcatattgta aagagtaact
     2221 agcacactta taaagttctt ttactgtatc tccatgtttt ggatatatta cacaaccagc
     2281 agaaattgtt atatttaatc catattttct ccatagttga aggtgtataa tcatattctg
     2341 tatttgatta tatagagtta gaatttcatt ttcttcaaca ttatcaacaa gaatgcccat
     2401 ttcgtcccca tcaagtttat aaagttcagc attatctggt aaaatagatt gtatagactg
     2461 tgctagtgtt tttattatat tatctccaaa atccctatca tacatctcat taacattttt
     2521 aaaatcgtca atatctaata atactatgca taatttttca atcataggat ttgaaatttt
     2581 atcttcaaat acactcataa attcatgata attcagtaac tgtgtcaatg aatcaatctt
     2641 attttgttta cctaattgcg tcattattcc tgcaaaaata cttggttctc caaattcatc
     2701 acgcattagt tgacctctac atcttaacca tatatattct ccactacgat ttttagccct
     2761 aaattccact gtatgataat ccatctggtt tttacctatt tcagtgtttg acttataaaa
     2821 acgattccag tcttctggat gtacaatttt tttccagtaa acaagcggat tttcaacaat
     2881 ttctcctggt aagtcaaata attcaacttg tgatggtgaa tatcgaaata cgcctgtttt
     2941 catattgcaa atgtatatat attcatctgt acctctaatc aaagcatcat aaagcaatgt
     3001 agaattataa tcaaaattgt ctcttgaaaa attaaaaact cctttttctt ctttattatt
     3061 tgatatttca tgaaattcat taatcttaat tggtttattt aagcttgttg agtttgcaat
     3121 atctattgat tgatgcatat gaacaactgt gccatcttgc catgtaatta aactatcata
     3181 attttcaaaa acacgattta acttattaca tttttcatac cactttatta atactccttt
     3241 tttatcattc tttaataatt ctaaaacttt acaaaaacta caagtagaat ttttttcagg
     3301 atataatact tgccaacaaa cttttccttc tggatctaaa atattatatt cttctttcat
     3361 ttttttgttc ataaaaataa tttcattagt atgaa
//
LOCUS       DACULQ010000259         3391 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.261, whole genome shotgun sequence.
ACCESSION   DACULQ010000259 DACULQ010000000
VERSION     DACULQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.261"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            262..1155
                     /locus_tag="JA154_17655"
     CDS             262..1155
                     /locus_tag="JA154_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT7997653.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1333..2157
                     /locus_tag="JA154_17660"
     CDS             1333..2157
                     /locus_tag="JA154_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT7997654.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEINQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2283..2864
                     /locus_tag="JA154_17665"
     CDS             2283..2864
                     /locus_tag="JA154_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7997655.1"
                     /translation="MKADSTKEQLINATIQLLSSHRDVSSITAREIVAKANVNLAMIN
                     YYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFDFIGEHSQFI
                     KISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSFIQLLFLRSN
                     AFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            2968..3144
                     /locus_tag="JA154_17670"
     CDS             2968..3144
                     /locus_tag="JA154_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997656.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
BASE COUNT         1286 a          390 c          463 g         1252 t
ORIGIN      
        1 tttaataaaa atctaaagta atttattaat atcactaatt gcaaatttat tgacctattt
       61 aataattaat aataatataa tatattcaat attaaatact aattaattat atttagtgta
      121 aaaataatta atcaaaatat aaaaaatagg atattattat aggcaataac taacatcaag
      181 cgataactta atacatataa aaataaataa acaattaata aaaattaaaa cttcattata
      241 aaacggaagg atagtgaaaa aatgcaacta tataacaccc cctttaatac aaacaaacac
      301 ttaatctatt tctttacatt tacgctagta ttgaactgga tattcagtct tacacctgcg
      361 ttgctcggaa ttacaaatac aatacttgga gaaatcattt tttatcttgc atatacatca
      421 ccagctattg taggtctatt ttttatttct acaatctatc cacaaagtgc aaaacaagat
      481 tttatttata gatacctaag ctttaaagaa attagaataa aatggatact attaacaata
      541 ttattttttg ctttaacttt tattacttcg ttaataatcg gaaattactt taatgtaaaa
      601 acttctgata tagattggat acacatagca gtgtttagac cttctaaaat agcttatatg
      661 ctattagcaa gttttatatt gagtatactt agtcaagaag ctggatggag aggatatgcc
      721 attgacaaat tattagttag atttggattt ataggctctt caattatttt agggcttgtt
      781 tgtggaatct ggtatctagg gtcgtatttt acacctaatc aaataccata taatttggca
      841 caatactccc tatttgatgc ttttttattt ataccaaaca ttgtactttt aaactttata
      901 attaattttg tatatatcaa tacaagtaga agcattttat cagctgggtt agttcatatg
      961 atgtactatt ttttcaacat tcaattactt ctacactacc ctattaaatt atgtgtaata
     1021 gctcaatatg tacaaataat atttggttta atatttcttg tatatatgat ttcttctaat
     1081 aaatttaaac agaaggtaaa ttctgaaatt gaacagataa agtcggatga ttttgaattt
     1141 gagttagact ggtaatttta atatgaatta taaattaaga taatctatga tttgatacga
     1201 ttactactta aatataattt actctctata atttattgca acatctggaa aataattgta
     1261 tttccacaaa cgcttgaagt atataaaaac aaaatatata ctgattataa gtgaaaagga
     1321 ggtcatattt tcatgtttaa aataggagag gtttcaaaac taacacaaat ttctattcgt
     1381 atgttgcgtt attatgatga acttggtatt ttaaaacctg cagaaataga caaatataca
     1441 ggtcatagat tatactcagt tgaacagatt tcaatactac aaagaattgt attattgaga
     1501 gattcaaaat ttagcgttgc agaaatagca aatatcgtac ataattggaa tgatgaattt
     1561 gtaattaaag aattgaatag aaaaaagaat gaaatacaaa aggaaataaa tcaggaacaa
     1621 caaagaataa ataaaattga taaatttata gaagctataa actgtgataa agatgaaatt
     1681 cattacaatg ttgtctttaa aaagattcca agttataaaa tcatatcact tagagagata
     1741 attccagact accaatctga aggtatactc tgggaaaagc tatctaaatt tatcaaagaa
     1801 gaacatatag aagtatcacg tcaatccaac aataacatag ctttttacca tgatgaagaa
     1861 gtcaaagata atggtgtaga tattgaagtt ggaatggttg taaagaagat tggaaagaac
     1921 aaatctggat ttatatatcg tgagacagaa gaaattgata tgatggcatg tactatggtt
     1981 tatggtccat atgaaaatat agctggtgca tatgaatcgt tctgttattg gttagataaa
     2041 aatagtgatt atcaaatatc aggtataaat agacaaatcg gacataaagg tgaacataat
     2101 gaaataaatc ctgaaaatta tcttacagaa attcaaatcc cccttataaa agtctaatag
     2161 attagtactt aaatacttaa gtactaatca cccattgaca tcccaaaatt ttcatgatag
     2221 tatctaaacg tgtgttaaac atgtttaatt tttttatggg gaaactttta aggggaaaaa
     2281 ttatgaaagc agattcaaca aaggaacaat taattaatgc cacaatccag ttactatctt
     2341 cacatagaga tgtttcaagc attacggcta gagaaattgt agctaaagca aatgtcaatt
     2401 tagctatgat taattattat tttaaatcta aagaggagtt aattaatatt tctattaaaa
     2461 aatttttaaa gcgtcatatg gatgaatata aggctacaca tgatactaaa aatttagcac
     2521 ctaaacagca attaagggaa atactaattg cattttttga ttttataggt gaacatagtc
     2581 aatttataaa gatttctgta ccctatatat tgatacagga agaaattgtt tatccatttg
     2641 agatactacc tttaattaag gctcactatg aaaatcagaa taacaaaaaa actgaagctg
     2701 aatacagaac tattgcgtat caaataactt catttattca gttgcttttt cttcgttcta
     2761 atgctttttt caagtatagt ggtattgata ttcttaatat tgaacagaga aatggtttaa
     2821 tagattttca attagattta ttattggtag atattacaaa ataatactat attaattttt
     2881 tatgtacatt tttttatttt atggtaacgt ttgaaaatat tctcattgac taaaaatact
     2941 tgcattaaat aagggggagg aattttaatg ttttttacat ttataggagt aatatcagca
     3001 attttattgt tgtgtacaat tatatgtggt ttatggataa aacttaataa tcgtaataat
     3061 gactctaatt ctcattttac tttttcaata ctttcaattg ctttttcact gttaattgtg
     3121 gtggcctttt tagtaagaaa ttaatgtctt tgttatgctg aggtttatta ttatagattt
     3181 ttattattgc atgattttta ctatattata gtatttttat tatattacag ggtcttattt
     3241 attatagtgt tttgttgtta cagcatttta ttatattaca gagtcctatt tattatagga
     3301 tttttattat attgtaaaat cttatttatt ataggatttt gttatattac agacttttat
     3361 tatattgaaa tctcttattt attgcagtgt t
//
LOCUS       DACULQ010000260         3386 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.262, whole genome shotgun sequence.
ACCESSION   DACULQ010000260 DACULQ010000000
VERSION     DACULQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.262"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(152..535)
                     /locus_tag="JA154_17675"
                     /pseudo
     CDS             complement(152..535)
                     /locus_tag="JA154_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      761..858
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            913..1089
                     /locus_tag="JA154_17680"
     CDS             913..1089
                     /locus_tag="JA154_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997657.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1315..1515
                     /locus_tag="JA154_17685"
     CDS             1315..1515
                     /locus_tag="JA154_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAT7997658.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(1679..1891)
                     /locus_tag="JA154_17690"
     CDS             complement(1679..1891)
                     /locus_tag="JA154_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997659.1"
                     /translation="MVCKIFITLKTILPKTQIIVTTHSPHILQIDSKEEMIVLDMAES
                     DNVYKKELKLGEYGVLGWTNEGLYLH"
     gene            2266..2448
                     /locus_tag="JA154_17695"
     CDS             2266..2448
                     /locus_tag="JA154_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAT7997660.1"
                     /translation="MFKFYNNATLMFLPGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            2519..3364
                     /locus_tag="JA154_17700"
     CDS             2519..3364
                     /locus_tag="JA154_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997661.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNKDPKYNLNTI
                     KKHLN"
BASE COUNT         1279 a          410 c          425 g         1272 t
ORIGIN      
        1 acaatatata acatttattt ttttaacttt tgtcagtaaa atgtcagcaa aacactttat
       61 ccacaatttt tatataagtt ttccacaact tatcaccaat attatccaca taaaaaaggt
      121 agcacttata ttattagcta ccttttattt attaatcttt atatttccag ctttttatcc
      181 aacttcctat ttctattatt ttttcttcag ttatttcttc tccatcatgt aattttgaaa
      241 ttaattctga taaaggattg ttatgtaaac ctctaaaaac tctttttccc tctattttga
      301 gatattctct ctcttttatt aatactgtat agtgtacatg cctcccaatt ctctatgata
      361 ttaaaaaacc ttttttggtt aatctagcta aaagtataat tgtagttgta tgcttccaac
      421 catatgtttt ttctatttca tctgcaacat cttttgatat tacagtttta taaccagtat
      481 gccatatgca tttcataatt tttaattctg tatctggtaa ttttttattt agcataatat
      541 attccttctt tcaatttatg tcacttcttc aaatttatct agcttaaatt ataataggag
      601 ctttttagaa caaaaactat atttaatttt taccaggtac tctacgtgtt tagtctgttt
      661 ttaagtgtaa aataatgttc atatctataa atttatttaa ctttcaaaca atcctgttga
      721 ataataacta attctattgt attattttag ttgtagaata aaactaaatc ggcaaaacta
      781 gagaaattta gtgacgcaaa gctataggga ctaagactta tataaatatc ttatgagtta
      841 tgtcagccag ttgccaaaga gatagtgttc tttttgtttt tatgaaagtt ttttaggggg
      901 ataataattt atatgtttgg aaataaaatg gataaatgta cacatgtatt gactgcttat
      961 attagcagtt cgtatgatta ttgtaatttt ttagatacac agttagatga ttttatatta
     1021 gagtacggag aaaatgtagt agagtcttgt ttacaccaag taatggtatt ggtaagtaag
     1081 tataattaga tagacattga tttctagaaa acccttttta gttttatcta catttatatt
     1141 aatatatagc aatgtaaaac gtttaaatat atttttaata ttaagaataa ttaaaatgtt
     1201 aactaaaaaa tataattaag tagacatttc tattaatcta tgatacaata gtatttgtgg
     1261 taatacacaa ttaaatcaag tatatcttga tataggttaa taggagtatt tttaatgaat
     1321 aatggaatag tgaaatggtt taataatgaa aaaggattcg gttttatatc aatggaaggt
     1381 agagatgatg tgtttgctca tttctcagct atacaaactt caggatttaa atcattagaa
     1441 gaaggtcaac aagtaagctt tgatatagtt aaaggtgcta gaggtcctca agcagaaaat
     1501 ataactatat tataatatat agatttttcg ggaaagactc tttttagagt ctttttttat
     1561 tgttaatata tgtaagataa tcattggtga agtaaataag gtatattgag taatgttctt
     1621 gatagtttat atgctaatga atttcaaaaa aataagcact cctataaaaa gagtgctctt
     1681 aatgtaaata taaaccttca ttggtccagc ctaatacccc gtattctcca agttttaact
     1741 cttttttata tacattatca ctttcagcca tatctaacac aatcatttct tctttagaat
     1801 caatttgtaa aatatgagga ctgtgtgttg tgacaattat ttgtgttttt gggagaattg
     1861 tttttaatgt aataaaaatt ttacatacca catagttgat ataaaactta agactagcag
     1921 actcataagc aattagttgc ctctctttac ataccacata gttgatataa aacctagagg
     1981 ctctcagaga agttataaga tgtttagtaa ctttacatac cacatagttg atataaaact
     2041 ccaaaataaa cttagcatct ccaatgttta cacatataca atgctcttaa aattgcagtg
     2101 aacgcgcaat attgcaattg ataatactta tcatatattc tcaatacctt atatttcaac
     2161 tgctaaacct caatttatca caaatatcgc tcactgcaaa atttctatat ttttattata
     2221 tcatagcata taattaatat tttcaattaa tttttacatt taattatgtt taaattctat
     2281 aataatgcaa ctttaatgtt tcttcctgga actaatataa aaactatttc agaaagatta
     2341 ggtcatgcag atataaaaat aactatgaat agctactctc agtttttaga agagatggac
     2401 agagaagcat ttgaaaattt gagcaaaata ctatttattg tattttaaag aagatgactt
     2461 attcaatatt tgatgctaga atataattaa taaaaattta attataggtg attgtattat
     2521 gaatattatt aaaagcataa ctttactctt aacattatta attgtgatat taatgacagg
     2581 ctgttcccca agagaaagtg ctagtgaagt tgcaaaaaaa tatattgata agatgaactc
     2641 tggtgactat gaaacagcat atagtttatt aagtaaagat agtaaaaaaa aaattagtat
     2701 agaaaatctt gaagaatttc aaaatatttt agttagtaca aaaagaataa ttagttataa
     2761 aataggtaaa gaaaaaatca taaaaaaata taaacatgat ggaaaagaat ataaaaatgt
     2821 tgttaaatta gaagaaactt ttaatataaa aaaccttctt tataaaaaag atgattctct
     2881 aaaagtaaat agatattttg tagttgaaaa taacaaatac aagctattat tgtatgaaag
     2941 tttcaaaaaa gaatttggta ttaatagtat ggaccttgct caacttaagc ttaatgattt
     3001 tggactaaaa tactttaaag aagttgacct tatactaaaa aaagctattg ctattaatcc
     3061 tactgattca aagctttatt atgcccaagc atgtaattat atgaattttg atggatttcc
     3121 tggttattca gatacaacat ataatgcttt agattcaatt aataaatctt taaagtattt
     3181 agataaaaat gattctgact acaaaaaaaa tgcaagtaat atatataact taaaaggagt
     3241 tattcttatg tttcacaaaa gatttgatga agcacaacaa tatttaaata aagccttata
     3301 ctttaacaaa gataataaag acccaaaata taatctaaat acaataaaaa aacatctaaa
     3361 ctagatattt aatttttgaa tttata
//
LOCUS       DACULQ010000261         3341 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.263, whole genome shotgun sequence.
ACCESSION   DACULQ010000261 DACULQ010000000
VERSION     DACULQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.263"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..494
                     /locus_tag="JA154_17705"
     CDS             <1..494
                     /locus_tag="JA154_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAT7997662.1"
                     /translation="FESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRV
                     EFKNSKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGD
                     NLVFEYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGI
                     IIK"
     gene            623..1906
                     /locus_tag="JA154_17710"
     CDS             623..1906
                     /locus_tag="JA154_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAT7997663.1"
                     /translation="MSKQMRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLI
                     GFVVADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCAT
                     TYEMGIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVL
                     IVKGIISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSD
                     KDQIGMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQV
                     GKVILGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSI
                     IAISVPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVP
                     FIQSLPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2108..3295
                     /locus_tag="JA154_17715"
     CDS             2108..3295
                     /locus_tag="JA154_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997664.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT         1202 a          381 c          630 g         1128 t
ORIGIN      
        1 gctttgaatc ttgtatattt agaaatgtgt cttttgaaaa ttgtaattta aaaaagattg
       61 atttacttga tgtggtattt gaaaactgca atttatcaaa tacatttttt gatggaggaa
      121 gtatatacag agttgaattt aaaaatagta aattgatagg agttagattt gatgattgta
      181 tacttaaaaa cgttttgttc aaggatgttt taggtaagta ttctaacttt tcattctcta
      241 aatttaagat agtttctatc ttagattcaa attttgaaaa ttctgtattt caagaagtaa
      301 aaggtgacaa tctagtattt gagtatacaa atttaaaaaa agcaaacttt aatggaacta
      361 aactttctgg catagatttt actactaata atattgaggg aattgagatt ggtatagatg
      421 atattagagg agcaattttt gatgtgtcac aagcaattga tttaacgaaa ttgatgggaa
      481 ttatcataaa atgaaaaaaa tattcataaa ataaattggg aaaacgttaa caatgtgtga
      541 atttttacca gattgacatt tttctgaaaa taaagtaaga ttatcttaat attcaaaata
      601 ttttcaaagg gggtaattta gtgtgagtaa gcagatgaga gatattttta tagttggttt
      661 tgctttattt tctatgttct ttggtgcagg aaatcttatg tttcctccat ttttagggat
      721 ggaatccggc aaagactggt taattcctct tattggattt gttgttgcag atgctggtat
      781 agcaatgctt gttattatag caacagcaag atgtaaggga agcatggatg atgtacttag
      841 gagagctggt aaagggttag caagagtaat gagtgtagct gctctagcat gtttagcact
      901 tcttgtaata ccaagaactt gcgcaacaac atatgaaatg ggtattatgc ctatatttgg
      961 agaccatggt ccagtagcta aagctgtatt ttcaataatc ttctttgggc taactttagc
     1021 atttactatt aggtcttcta aggttataga cataataggt aagtatttaa ctccagcgtt
     1081 attattagta ttatttgttt taatagtaaa gggtataatt tctccagcag gacctatgag
     1141 tcctgagcat atgatagata aaaatctatt tggtgaaggt atatcacaag gttatcaaac
     1201 aatggacgct ctaggtgcag catctatggc aactataatg ttgatgtcta taatagctaa
     1261 aggatataca tcagataaag accaaatagg aatgactatt aaagctggtt ttgtagcctg
     1321 tgtatttcta gcagtagtat atggtggtct tacttattta ggtgcaacag tttcaacttt
     1381 atatgatact agtatacctc aagcatcttt attagttgaa ataactagta tattgttata
     1441 tggtcaagta ggaaaggtaa ttttaggtgt aattgtggca cttgcctgtc tgactacttc
     1501 tacaggttta acagcatcaa tatcaactta ttttgaagga ataagtaagg cgagttataa
     1561 acaactggtt ataggtatat gtgtagctag tatgcttata tcaaatttag gtgttgattc
     1621 tataatagcc atatcagttc caatcctaca aactatatat cctgttttat tagcaatagt
     1681 tgttatggag ttggctggta aacatataaa gaatgataat gcttttaaag gtgcagcata
     1741 tgttacatta gtgattagtt tattatcagc tataaatgga atgacaggag ctgttccatt
     1801 tatacaaagc ttgccacttg caggtttggg ctttaactgg gtaataccag ctatattggg
     1861 tggaatagtt ggtaacttta taaaaagcaa caaacaagta gcataataat ttgtaattaa
     1921 tttaatttta taatatatta atttttagta ctatgggtag gtttaatcta aacctaatct
     1981 acccatagta ctttttagat tggattataa tagcatgtat ataatttttt ataatataat
     2041 ttttctttga agtatgataa aattagtaat agtgataaga atgtacaaat ttaggaggtt
     2101 ttaattaatg cttagaaaaa taacagcatt tatgctatcc atatgtttaa ttattggact
     2161 tacaggttgt agtttacttg taggcaagaa agataaagag aaaattatag atgattttaa
     2221 agttgcagtg gtaactcaac cattatctga aaataaagta caatacaata tggtggaaga
     2281 gatggcaaaa gaatatgagg aagagaataa aattgataaa gacaaagatg gtcaaactaa
     2341 agttaaacaa actataaagc atgttgtatt gccagagaat tttacttcta atatagacag
     2401 tgctattaat aagatagtaa agttggctga cgataaagaa gttcaggcta ttgtagtttc
     2461 aactgaccaa gcagggcttt tacctgcttt acaaaaggta aaggaaaagc gtccagaaat
     2521 aataactatt tctgccccta tgggagatga taaaaaccaa ttaagccaat ttgttgatgt
     2581 gaatctaggg gttagtgcag aggaaagagg aaaagtatta gctgaacgtt caaaagaaat
     2641 gggagcaaaa gcttttattc attatgcctc tacagatgat ttgaaagatg ttaatatagc
     2701 caaaagatta gaaatgatta aagagacttg caaaaatata ggattgccat ttgtacaagt
     2761 taatactcca aatataaata ctgaagaaga caaaaacaaa gttaagcagt ttttaaatga
     2821 agatattgaa aaacaagtta aaaaatatgg aaaagatata aatgtatttg gtgtaaatga
     2881 gtatatggat gaagttatat tgactaaggc tttagaatta aagtatatag ttgcagagca
     2941 aagcaatcca tcaccaatac aaacatatcc tagtgttatg ggtctaaaaa tatctgaaaa
     3001 agatgctcaa aattatgata aaataaacga catgataagt gaaaaggcta aggcttttgg
     3061 catgtcaaat agacttggtg gatatcctat gccaatggat gcttttctac catcagtagc
     3121 aatttatctt gctactgaaa tggtgaaaca agatttaaca caagaagatg tatgtgaccc
     3181 agattattta gaggcgttta cagaattaag atttggaatc ggttcagaat ttactccttt
     3241 aacagaggta ctttataatt atcaatcagt aattttaagc caattaattt attaatatta
     3301 aggaagtgtc tcaaaatgaa ctttttagtt tatgaggcac t
//
LOCUS       DACULQ010000262         3312 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.264, whole genome shotgun sequence.
ACCESSION   DACULQ010000262 DACULQ010000000
VERSION     DACULQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.264"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..939
                     /locus_tag="JA154_17720"
     CDS             <1..939
                     /locus_tag="JA154_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT7997665.1"
                     /translation="SANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSID
                     NNSKEKAIKLIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNE
                     LIIDVANPLDVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGFVTYRTNWWYTKKDD
                     LSSKLFYEQKLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIK
                     QEIYNLYKAHRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFEN
                     EDWINGFSVFCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            993..2117
                     /locus_tag="JA154_17725"
     CDS             993..2117
                     /locus_tag="JA154_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAT7997666.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGMQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2195..3169
                     /locus_tag="JA154_17730"
     CDS             2195..3169
                     /locus_tag="JA154_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAT7997667.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         1384 a          330 c          582 g         1016 t
ORIGIN      
        1 tcggccaatt tatctgtaga ttatgaaata aatcaagtta tgaaggatat agataaattg
       61 ggattgaata cagtaaatgt acctataatt ataaatgtag agtccattaa ttctgatatt
      121 atgtcaatag ataataatag taaagaaaag gcaattaagt taataaaaaa attaaataaa
      181 aaaggtattt caacaatatt agaggcatat ccatggattg aaaatggaaa attgtatgag
      241 actgactgga accctataaa taaaaaaaga tttttttata cttggcaaaa tgccatatta
      301 aatgaactta taattgatgt agcaaatcca ctggatgtaa atgttttaaa tattggttct
      361 aattttgtac atttggaaga atatcaacaa aattggggtg aaataataga ttttgtacaa
      421 tccaaatttg acggttttgt aacgtacaga actaattggt ggtatacaaa aaaagatgat
      481 ttaagtagta agttgtttta tgaacaaaag ctgaacaata atttttttga taagttagat
      541 tttatatcta tagcagcata ttttgagtta agtaataaac ctgttaatac agtggatgaa
      601 ttaatttctg ccttacactc atctactgta aacaatagag gacaaaatat aaaacaagaa
      661 atatataacc tttataaagc tcatagaaaa cctatatttt ttggagaatt aggtttttca
      721 aatagagaaa gtgcaagttc tcagccgtgg aatcatactc cttcaaaagc tgtaaatggc
      781 gaagaacaag caaggtgttt tgaggcatat aagaaagttt ttgaaaatga agactggata
      841 aatggttttt ctgtgttttg tgtaggtaaa atagatgacg aaaaaaattt ttacccatca
      901 aaagagagta taaaagttat taagtcttgg tatgaataat tttgaaagtg tagaatttat
      961 atagattaaa tagatgatta ggagagacat aaatgaatga catcaaggtt atgactgtat
     1021 ttggaactag acctgaagct atcaaagtag cacctttaat aaaggaactt gaaaaaagag
     1081 agaatataaa aagtattgtt tgcgttacag ctcaacatag agaaatgctt gaccaagtta
     1141 tagaaacctt taatataaat gtagactatg atttggatat aatggaaaaa ggacaaagcc
     1201 tgaatgatat aacatgtaag attttaaata aacttccctt gattttaaac aaggaaaatc
     1261 caaatataat attggtacat ggagatacaa ccacaactct agctacaagt ttgactgcat
     1321 tttataataa aactcttgtt ggtcatatag aagctgggct tagaacctat gataaatatt
     1381 ctccttttcc agaggagtta aatagacaac ttacaggtat tattgctgat atgcactttg
     1441 ctcctactaa tttggctaga aaaaacttga tttctgaagg aaaacctaat aataatatat
     1501 ttgtaactgg taatactgct attgatgctt taaagatgac aataaaagaa aactataatc
     1561 atcctataat tgatgagatt ggtaacgata ggatgatttt actaacctct cacagaagag
     1621 aaaatttggg taaacctatg aaaaatatat ttagagctat aaagcgaata gtagatgact
     1681 ttgaagatgt acagatagta tatcccatac atttaaatcc taaaatacga actatagctg
     1741 atgagatatt tggaaaattt cctgaaaaaa ttcatattat agagccttta gatgtagctg
     1801 attttcataa ctttttaaat aaatcttata tgataatgac tgatagtgga ggaatgcagg
     1861 aagaagctcc atcattaggt aagccagtcc ttgtgttgcg tgataaaaca gaaagaacag
     1921 aaggaattga agcaaagaca cttaaattag taggtactaa tgaagataga atatataatt
     1981 cagtgagcga tttattaata aataaagata actatgttca gatgagtaaa gcatctaatc
     2041 catatggaga tggaaatgca agcaaatata tagtggatat aataattaaa aaatttaatt
     2101 gcaaatattt aaattagata agacctaaaa cattaactaa ttgatataag atttatgata
     2161 atattaagga atacaatttt taagggggag taatatggct agaaaattaa taaaaaattt
     2221 gggtaaaagt aaaagtgtaa agagagtgaa acttttattt aagaagattt ttattactgt
     2281 attcatagta gcaagtatag ttgctatttt taatataacg aaatattttg aggaattata
     2341 caaagtaagg gatttaaaaa gtactaaaat tgaatactat atggatgtgg ctgatgaagc
     2401 aggagatgga aaggtccaat taagttggaa ggccttactt gctatagaca tggtaataca
     2461 tgatgaagat ttaagtaata taaaaaagaa agacacattg gatatagggg aaaagtttat
     2521 agtagaagat aaaaatgata aaggcgaaaa ggtgtataag gtaaaaaagt ttaataaggt
     2581 attaagcgaa ttgaaatttg actcttctca aaaaagtaga gcaagaaaat acatgaaaga
     2641 tttagaatac acataccttg gaaataaaca attagatagt agtgatgaaa aaattaaatt
     2701 tataaagaag ttagaagact cagctataag agaatatatt gattatggaa tattgccctc
     2761 tataacaatt ggacaagcta tattagaatc tggttgggga aattctaaac ttacaaaaca
     2821 gagtaataat ttatttggta taaaagcaga taaagcatgg aaaggaaaaa gtgtagaaat
     2881 ttcaacttca gagcattata atgaaaaaat tgtagctagt tttaggtcgt ataattcatt
     2941 acaagattct gtcaaagacc acagtttatt tttaattaat aataaaagat atagaaaaca
     3001 tggattgttt gaggcaaaag attatattag tcaagctcaa tcattagaaa atgcaggata
     3061 tagtacagca gaagataaaa aaggaaatcg tatatatgca gagctattaa tagatgttat
     3121 taggagttat aatttgcaat tgatagataa taaagttgag acaaaataat tgttaacttt
     3181 atatgaggag ataacaagat aagattcttt tctacaaaaa gaatagaaaa attgtaaaaa
     3241 aaactaaaaa aatgtattga cttgttattt aaaaggtgat attattatta ctgtactcga
     3301 aagagaacaa aa
//
LOCUS       DACULQ010000263         3291 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.265, whole genome shotgun sequence.
ACCESSION   DACULQ010000263 DACULQ010000000
VERSION     DACULQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.265"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..357)
                     /locus_tag="JA154_17735"
     CDS             complement(<1..357)
                     /locus_tag="JA154_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7997668.1"
                     /translation="MKDDYIMRRVIVYSVLSVLIVTLVFILFEYSRLHKLYEHQIKNT
                     QNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLENKMSNDKNFSLYL
                     KSFFKESFFIFLMMFIV"
     gene            complement(387..1094)
                     /locus_tag="JA154_17740"
     CDS             complement(387..1094)
                     /locus_tag="JA154_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7997669.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            complement(1399..3240)
                     /locus_tag="JA154_17745"
     CDS             complement(1399..3240)
                     /locus_tag="JA154_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAT7997670.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
BASE COUNT         1036 a          590 c          281 g         1384 t
ORIGIN      
        1 tactataaac atcattaaaa aaataaaaaa tgattcctta aaaaaagatt ttaagtataa
       61 gctaaaattt ttatcatttg acattttatt ttccaatcca tagccaaatt tcttaaatgc
      121 cttttctcca gtagacacct tactataatc agaattataa atagaattta ctatatctat
      181 ttcatcatta ggatattttt caatcaatat tccagcaata ttttgagtgt tttttatttg
      241 atgttcatac aatttatgta atctgctata ttcaaaaagt ataaatacaa gggttactat
      301 tagtacagat aaaacagaat atacaataac tcttctcatt atataatcat ctttcacact
      361 taaaccatct ttgaggttca tattttttag cattttttta tactcctttc tccccacata
      421 tatccaaccc ctcttactgt ttttatatat ttaggttcag atggattttt ttcaaccttt
      481 tgtctaagtc ttcttatatt tactgcaatc gtattatcat ctacaaaatt accactacta
      541 tcccataaag attctaacag ttgctcttta gtcaatgtct gcatagaatt ttccatcaaa
      601 tattttaata gttttgtctc tgtcttactt aaaattatct cttcttcctt atcatctgtt
      661 cttataatta atttattttc tatataataa aagaataaat catcacaaac taatagagta
      721 taatttttta ctgtatttac tcttttcata agcgcattca cttttgatat taataccatc
      781 aagctaaaag gctttgtgat ataatcatct gcacctaaat catatcctgt gacaatatcc
      841 acctcttcat caagagctgt caacattatt atgtaaacat cacttttctt tctaatctcc
      901 tcacaaaagt caaaaccact accatcaggt agacctatat cggaaataat taagcatatc
      961 tcttcttttg cgaaaatatt ttttgcttct tctataccaa atgcactaaa aacattgtat
     1021 ccttcttttt ttaatttaaa actaattcct ctatttaaag atttgtcatc ttccaaaagt
     1081 aatatattat tcatccctat atcctccatc atcatatata ctagctaaat tatatcatac
     1141 taataaaatc atttttttat caatttgtaa atatatcaga agttgttaat atgatttcat
     1201 ttacttgaat aaaaactaaa aatccccata atactatgcc atttagtgta attatttttc
     1261 ataaaataca attttattta aaagtaatca tgttaaaaca taaaacaaag gaaattagta
     1321 tttatataac taatttcctt tgctgtttac tattatctaa aattaaatta ctatctacta
     1381 atttttgctc attaattttt attttgactt tttatccttt aactcttttt gattaaaatt
     1441 caataaacat atcaatgtac ttgaaactat aaatataatt atactatttg aaaaacctaa
     1501 atctcctgtt ttaggagatt tttctttagc ttctctttta gatattccta gattagaact
     1561 ttctctagca cttgttttat ctaagctttt atttttatca ctatcagagc tttttatatt
     1621 attagataga ttctcttcat tatcattgat atttttttca ttttcactta atatcaaatc
     1681 actctcatga ttcttgctta ttaactcatc tttagattta ataccaattc ttccatctac
     1741 attttttgat atttcttttt tgtactctat atattcaatg actgcactct ctaaatttcc
     1801 aaattccttt agtactggct cttttgaaaa acaaggaaat tcatctccac ctgatgctat
     1861 ataatcattc gttgcaacta catacttttt atttaaatct aatggctttc cttctttttg
     1921 aatatttgtt attttttcgc cttctttttg tttaggatta aagtaatatg aaattcctcc
     1981 cacttgcaaa aaggctgaac ttttttctgg ataaagtttt actccatgct ccaatacatc
     2041 tttaatctgt gctcctgtaa gttctttggt aactatagta ttgctaaaag gaaatacatc
     2101 tacaatatct cttcttgtta tattgccttt ttctatcgta tctcgaatat tccctccatt
     2161 aaataaagaa atatcagcct tagttttatc tagtaaaatg tcacttatta aattacccaa
     2221 attagtttct tttgtcctta cattttcata tgaaccatct agagtatttg tagttacccc
     2281 tataacttta tctaatattt tttcttgttg ttcttttatt tcatcaacct ttttacttac
     2341 agcggagtca agcttatact ttaatgcttc ttcttttcca attaatctaa ctgatttatt
     2401 ttctattttt atattttcta catcattagt atccaaagtt agttttatct cgccaatatt
     2461 ttctaaatat tgtcctgcac ttacaatcaa tgtatcaccc acttgtttac cattttcaaa
     2521 cttagtgtgg ctgtgtccat caataatcaa atcaattcct tgaacctcat ttgctatatc
     2581 tatactattt ggcttactta aactatttgt accaatatga catattgcaa ctattacatt
     2641 ggcacccttt ccttttaatt catttacttg ttctgttgca ctttctatag gatttttaaa
     2701 ctctaatcct tccacatttt taaatccagt tttgctcttt gtttcctggg ttgagagtcc
     2761 aaaaaatcct atttttattc catctatttc ttttatatca ttactatcaa aaactttttt
     2821 tccattttca aaaatatttg atgataaaaa ttttaaagta ttctctccta aattagcact
     2881 tttagataac tcaaacagtc tatcttttcc ataattaaag tcatgatttc caggagtaat
     2941 atagtcatat ccagcacatt taattaattc aattgcactt ttccctctat ccatatttac
     3001 tattggaagt ccatgcaaac aatctcctgc atctactaaa attgagtttg gagtttcttt
     3061 tttcaatgtg gctagatttc ctatttgtat atggtcatca ccttcagcat atcttccatg
     3121 tatatcatta gtgtgaaaaa tggttatttc ttcaaatcgc tctagcccat aagaaataaa
     3181 actctgactt gttgtaatta gaattataat taccaaaatt aaataccttc taatcttcaa
     3241 tataaattag cctcctcaaa ttagttttag ctttaatata aaatttatat t
//
LOCUS       DACULQ010000264         3196 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.266, whole genome shotgun sequence.
ACCESSION   DACULQ010000264 DACULQ010000000
VERSION     DACULQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.266"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(173..850)
                     /locus_tag="JA154_17750"
     CDS             complement(173..850)
                     /locus_tag="JA154_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAT7997671.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(1039..2403)
                     /locus_tag="JA154_17755"
     CDS             complement(1039..2403)
                     /locus_tag="JA154_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT7997672.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2531..3052
                     /locus_tag="JA154_17760"
     CDS             2531..3052
                     /locus_tag="JA154_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAT7997673.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         1242 a          506 c          449 g          999 t
ORIGIN      
        1 aacttattag aaacaaaaaa tatacataaa aaaataaatg taaatatcaa ataagatact
       61 atataaaaat agatataagt atctttttta ttattgaaat tatgaaaata tatttgattt
      121 cacacaaaaa agagtgcctc atgaactaaa aagttcattt tgagacacac ttttacagtg
      181 tcaaagaacg aagtttatcc ttatccaata cttttatacc accacgagat aaagacacaa
      241 taccttctct agcaaaatat ttcaacattc tcgatacaac ttctctggca ctacccatat
      301 acttagcaat ttgctcatga gtcatattta tagtgctact gccattttta gctatctcat
      361 caattaaaaa agatgccaaa cgcttatcaa aactcataaa aagaatttgt tgcatagccc
      421 acataacatc agaaaaacgt tcggtagcta atttatatga aaataactct gcatgaatat
      481 tttctgtaga taattttgca aaaacagagg aactaatctg tataatatcg cagtccgttt
      541 cagcatctac atagacatca aaagttattg tttttagaat acaggaagct gataaaatac
      601 atacatctcc atcatcaagt cggaacagtg taacctctcg accttcatct gaaagtatgt
      661 atgtacgtac tgttccagac tttataatca aaataccaat acagtcacta tcaccacaat
      721 gtactggatt cccttttttg taatgcgagg tatttgctga agaaattaat aattcttttt
      781 gtaagtcagt aagtttatcc caaaaaggca agctatgtgt aagtatatcg atttcatttt
      841 gtgaaatcat acaatatctc ctttcttaaa ttgttataat cccatgatac aagtgaatag
      901 ataaattgac aagtagtttg ttaataggat tatttagtca gatagtaata gtatgattta
      961 tgtagaaaaa atatgataat atatattctt gctattaatg agaaggtaat atgttttgat
     1021 gatagtaggt ttgtataact aatgcatacc gtttattttt aaaagattaa gttccttatg
     1081 taaccttata gacataaaac cagcaattat agcagccatc aaatcagcta ttggttgtgc
     1141 atataagata ccatttaacc catagaatac aggaataatc aaaattatag ggataaaaca
     1201 gacaccttgt ctacacatac ctaaaaatcc accttccttt gcttttccta gtgcgaggaa
     1261 taatgtagaa tacactgtat agaatccaaa tagcataaat gaaaacccat ttgccctaag
     1321 agctttacta ccattttcta ttaatagaat gtcaccactt gtaaattgtg aaattataga
     1381 ttttgggaag agtatcaata taagaccaag cactacacaa aatattgtag tccataatat
     1441 ggaagttttt attgctatat gtaatctatc gtagttcttt gaaccaaaat tataaccagc
     1501 aattggttgg aaacctttta taaaaccaaa tacaacaaga ctacctaaag acgttattcg
     1561 tgttacagca cccattccag ctatcacaga atcaccatac tttgtagcct ccatattagt
     1621 taatgtaatg gcaagactag tcaataattg aaatattaag gtaggtatgc caatttttag
     1681 gatttctgaa aaaatttctt ttgaaaaaca gcattcgtta attctaaaac taaatatact
     1741 tttcttgctt aaaatatatc ctacataaac aagtgttgaa gcaatctgtg agatagctgt
     1801 tgcaatagaa gcacccctta cccccagacc aaaagtataa atgaatattg ggtctaaaat
     1861 catattaagc aaagcaccta ataacatagc acacatagtt gttttagcag ctccttcact
     1921 tgtgacaata ttagtcatag tgacattgaa tatattaaaa atagatgaca cgacataaat
     1981 gctagcataa gatattgcat aaggcataat actttttgta gctcctaaca attttaaaat
     2041 tggattgaga aaaatcataa tcacaataat aactaatgta cccactataa tactactgta
     2101 aatagatgta cttaccactt ttcctgcttt ttcattatca ccatggccta aaagtctggc
     2161 aatatatgaa gcagctccat ttccaaacaa taatcctaat cccacaacaa gctgacctaa
     2221 tggaaatgca acggatatag ctcccatttg acttgttcca agtccaccca caaagtatgc
     2281 atcaaccaaa ttgtataatg cagttatcat catacctatc atggtaggta atcccataga
     2341 taacaatgct tttgagattg gggcagaccc taataattca attcttttgt tactttcttt
     2401 catacttaac actcctcttc tattgacatt aagtctaaat tataatacta taaattatag
     2461 taagtatgga aaatagtact ataatttata gtatttgtca atagtaagaa aactaaagga
     2521 gatgattttt atggctacaa tagatttaat tgtattggga atagtaaagc aacaacccaa
     2581 gagtgcttat gatattcaaa aggcagtgga atacaggaat atatcacgtt gggttaaaat
     2641 aagtacacca tcaatttata aaaaagtaat tcaattagaa caaaagggat atattgaaag
     2701 taaaactgtg aagaatggaa aaatgccaga aaaagcagtt tattcattaa ctgaattagg
     2761 tgagacacat tttttagatt taatgtttga tacatctagc aaaccaataa atattctttt
     2821 agattttaat gctgttatag taaatttaaa tagccttaca ccagaaaaac agaaattatg
     2881 tctcaataat attgaagaga atgttcaaga gttaaagaaa actattgagg aaaatataag
     2941 tataaagaca gaagtaccag acacagcaaa agcagtgttg gagcaacagt tggtgctagc
     3001 acaagcattg gaaacctgga taaattcttt aaaagataat atgggaaatt agaattagag
     3061 aagctaactt aataatcatt aaacaaaaac cagcatattt aataatgaga tattagaaaa
     3121 aataaaacaa atgatataaa ctgaaaagta tatttaaaat aaagctcact taattgaggg
     3181 agaatgttaa actttt
//
LOCUS       DACULQ010000265         3177 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.267, whole genome shotgun sequence.
ACCESSION   DACULQ010000265 DACULQ010000000
VERSION     DACULQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.267"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            100..1320
                     /locus_tag="JA154_17765"
     CDS             100..1320
                     /locus_tag="JA154_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT7997674.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAVVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1357..2706
                     /locus_tag="JA154_17770"
     CDS             1357..2706
                     /locus_tag="JA154_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT7997675.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKKGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFVGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(2838..3131)
                     /locus_tag="JA154_17775"
     CDS             complement(2838..3131)
                     /locus_tag="JA154_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997676.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
BASE COUNT         1216 a          426 c          478 g         1057 t
ORIGIN      
        1 caatattctt ataaaaaaca aaattctcca tcgaaaatta tggcataata attgcttatt
       61 atattaataa aacaataaaa agcaattgga ggaataaaaa tgcaacaggc tttgaaatta
      121 aaatatcaaa ccaaagaatt aggaaaagat tttgtaatag aaagttgtaa ttctataaaa
      181 ccatggctta taaacataag aagagaatta cataaaattc cagaattagc tcttgaagaa
      241 aatcttacta aacagaaggt aatttcttat ttaaaagaaa tcggaattga ttatatggaa
      301 tttacaaaac acaatggaat tatggcatat attttaaaag aaagtgcaga taaaacgatt
      361 tgtataagag cagatatgga tgctcttcct attgaagaag aaaacaacat accttataaa
      421 tcaatacatt ctggaaaaat gcatgcttgt ggccatgatg cacatacaac catgctactt
      481 ggagcatgta aagttttaca ttcaataaaa gataaactta atgtaaatgt aaaattctta
      541 tttcaaccag cagaagaagg ttttggagga gcaaaatttt tagttgagga tgggtgttta
      601 gaaaacccca aggccgatta tatattcgga cttcatgtta tgccacatat agaaacaggc
      661 cttatcgaaa caaaatatga tacattaaat gctagtgtag acactataaa aataagtatc
      721 aaaggtaaaa gagctcatgg tgcatatcct gaaaacggta ttgatgcaat agttacagct
      781 tcacaaattg ttacatcatt acaaactata ataagtagaa acttagaacc aaataatgct
      841 gttgttttga ctataggtaa aatatatggt ggtgatgcac acaatgtaat atgtgaagat
      901 gtaaaattag aaggcacttt aagaacctta aatagcaaaa caagaaactt tatgattgat
      961 aaaatagcta aaattgttgg agatactgca tctgcatttg gttgtgttgg gactttacat
     1021 gtaagtgatg aaaactatcc tgctgttata aatgaaaaag aattagtaga tacagttata
     1081 tcaaatacta aagaattact tggagaggaa aaatttatat tgagaccaaa cccgtcttta
     1141 ggtggtgaag acttttcttt ttacacagaa cattgtaaag gagcattctt ccatttagga
     1201 tgtaaaaatg aggaaaaggg cttaatttca cctcttcata cctctagctt taatatagat
     1261 gaagattgtt taccaatagg tgttatgatg catgtaatga atactttgta ttttaattag
     1321 tacagtaatt aataaaagaa taggagataa attaatatgg aaaaagctaa gaaattaggt
     1381 ctatttagta tgatattatt agggattaat agcataatag gttcaggtat attcttacta
     1441 ccaggtaaag tttataatct agcaggtcaa aacagtatgt ttatatacat ttttgctaca
     1501 ctcttagttc tatcaatact tttatgtttt gcagaagttg gaagcatgtt tgataagaaa
     1561 ggaggagcat atctatactc caaaaaagct tttggagatt ttataggatt tgaagttggt
     1621 acaatgtctt gggttattag aattatatca tggtctactc tagcagtagg atttgcaaca
     1681 gctttaggtt cattttggcc agaatctgcc actgaataca aaggttatat agctgcaata
     1741 ctcgtaacac tactttcaat aaatagctta tttggaatta aaagcacaaa gataatgaat
     1801 aatgtaataa ctatagctaa attagtacca ttaatagttt ttataatagt tggaatattt
     1861 tttataaaat ttgtaaatat tgttccttca ggaaatgtag ttaactctag tatgggtcca
     1921 gccattatat tagtatttta cgcatttacc ggctttgaat catttattgt ggcaagtgga
     1981 gaaatggaaa atcctaaaaa aaatcttcca gtcgcattaa taaccacaat atttatatgt
     2041 gcaataattt atatattaat tcaaattgtc tgtatgggta tacttggaga tagactattt
     2101 gaaaacagta ttccaattgc agatgcttca agtgtatttg taggcaatta tggtaaagtt
     2161 ttcatatctg tagcaacatt aatatctata tttggtataa atataggttc atcaatagta
     2221 actcctaaat gtggttcttc tctagcagaa gaaggttctc ttcctgcatt tataggaaaa
     2281 acaaataaat atggtgctcc atatgtagca ataataatat ctttaatttg ttgtatacca
     2341 cttgtgctaa ctggaagttt tgaacaactt gcagtaatga gcgtaattgc aagatttgct
     2401 cagtacatac caacttgttt atcagtaata gttttaagaa aaagaacaga tgttaaggcc
     2461 tcttttaaga ttccatttgg ccctgttata ccaatagtag ctattttagg tagcttatgg
     2521 ttgttacaac aagcttgggc agaagatatt tcaaaaccta taactcaaaa tagagtttta
     2581 ataggattag gggcaatgtt gttaatagct cctctatata tatttatgaa aaaaaataag
     2641 gaaatcgagc ataaaaataa atcagaagat tctaaattat atatttcgag agaaaaaatt
     2701 caataatatt ttatactatt acttctcata aaatataata aatataaaaa aattgtagat
     2761 aaaactattt tataaaattt atctacaatt tttttatata aacatatatc aaaatcaaaa
     2821 tattattaat ttatacttta aagattccca tctttatcat attttattct tataagaaaa
     2881 tataatccta ttactactaa ctctatcact aaaaatactc cataggtaag aattgaatca
     2941 atactatcta acccatttct aaaaaaaatt aatgctatac ctgctgctat aatccctata
     3001 gcattttttc ttactgcatt ttgcccaact ccataaaaac ctggcatttt tccttttatt
     3061 tcgacagcag ttaaaattat cccaaacact ataaataata ctccaataag catcataatc
     3121 atactaatca tctaattacc tccttcactt aaggaaattt caatttctaa aatctaa
//
LOCUS       DACULQ010000266         3176 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.268, whole genome shotgun sequence.
ACCESSION   DACULQ010000266 DACULQ010000000
VERSION     DACULQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.268"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(46..618)
                     /locus_tag="JA154_17780"
     CDS             complement(46..618)
                     /locus_tag="JA154_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAT7997677.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(647..913)
                     /locus_tag="JA154_17785"
     CDS             complement(647..913)
                     /locus_tag="JA154_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HAT7997678.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(913..1122)
                     /locus_tag="JA154_17790"
     CDS             complement(913..1122)
                     /locus_tag="JA154_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997679.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1391..2251
                     /locus_tag="JA154_17795"
     CDS             1391..2251
                     /locus_tag="JA154_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAT7997680.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2299..>3176
                     /locus_tag="JA154_17800"
     CDS             2299..>3176
                     /locus_tag="JA154_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAT7997681.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMK"
BASE COUNT         1211 a          370 c          521 g         1074 t
ORIGIN      
        1 taaattataa attatcaaat aataatagtt atacaaataa aaattttaga acattttttg
       61 agattcttct aactcgtaag catccattaa ggcttcacca aatctttgat aatgaattat
      121 ttctctttga cccaagaatt ttaatacatc tataacatca acgtcatctg ttaggtttat
      181 aagttgataa taagttgcaa gtgctttttg ttcagctgcc atatcctcat gtaaatcggt
      241 tacgggattt gacataactg ctataggtct tacatcaaat ggaacaccat ttgaatctgt
      301 tggataaatt ccatatccat tttgagcata gtttgaacca agacctgcag cttttaactc
      361 ttctgggctt gcatcagaag ttaactgata aaccatagta catataagct caacgtgagc
      421 tagttcttct gtaccaatat ctgttaaaag tgcacgcata ttaccagtag gcattgtata
      481 tctttggctt aaatatctta aagatgcagc aagttctcca tttggcccac caaattgagt
      541 tataagaaat ttagccattc tagggtcaca agtttttata ttaactgggt gttgtatagt
      601 tttctgatat atccacatga attttcaatc ctccaatact tgatgtctaa taattaaatt
      661 ctctgtccca aggccaaggt tggtcagccc attgccaagg gcatctactt ggagcatacc
      721 caaagttagt taaaggaccg tatttatttt cgtaagcata tctagcttgc gcaaattgat
      781 tacataatga attataagtg ttgatagcat ttttatcatc agggtgattg tctaaatata
      841 agttcatatc aacacatgca aaagatgttt cctgaacttt tctcataagt tcagctctag
      901 atgagtcttt cattatcttc tacctcctct atattttttt ggcttagcat ataattcttt
      961 gttgtacatt tcagactcta gtaagtttag ttctggaaat atagttccaa attttaatgc
     1021 atcagaaagg cagtacattt ctgtgaaaat ttgattatta atgtatggtc tagccaaaga
     1081 tggaccacaa ttttttttga tatctctagt cttattttcc aagatgaaaa cctccttgtt
     1141 gtaaatagat aggtaactct atctagtgta taatattcaa tcatttaaaa atcgttacta
     1201 ataataaaat attaaatgag aaaaataaaa aagataataa aagaaagagg ttatttatgt
     1261 tattataagt ttatgtgtta ttgtaaacta taaattattt agtaaaatta aagtaaaaat
     1321 tatagtattt tgtggatgaa ggtagacttt tttgtaaaat attattaaca taaatatacg
     1381 aggtgattag atgagtgaac tatcaggaaa aggctgtgaa attatagttc cttttgagga
     1441 aagacttcca gtaagagata tagaaaaaag tataataaaa aaatatagga aaaatttatg
     1501 gtctaagttt atgaaggcaa taagagatta taaattggtt gaagaaggtg ataagatagc
     1561 tgtagcgatt tctggtggaa aagacagtat tcttatggct aaaatgtttc aagagttaaa
     1621 aaaacatgga caagttaatt ttgatgttga atttattgct atggaccctg gatatcatgc
     1681 aaatataaga caattactta tagataattg tgagtatcta aatataccaa tacatttatt
     1741 tgattcaagg atatttgaga tagcagatga aatagcaaaa gattatccat gctatatgtg
     1801 tgcaagaatg aggagaggag cattatattc aaaggcagaa gaattagggt gtaacaaact
     1861 tgcattaggt catcattatg acgatgtaat agagacaaca atgcttaatt tattgtgtgc
     1921 tggaaatttt aaaacaatgc ttcctaaact taattctact aattttgaag gtataaaaat
     1981 tataagacca ctttattata taagagaaga acatattata agatttattc aaaatagtgg
     2041 tatatggcca ttaaattgtg catgtatggt tgctgctaaa aaaacaggta ataaaagata
     2101 tgagataaaa gacttaataa agagcttaga atcaaatttt aaaaatgtag aaaaatctat
     2161 atttaaggcg gcagaaaatg taaatcttga ttcagtactt ggttggcaaa aagatgggga
     2221 gaaacattct ttcttagaaa attttgaata aaaatagtta aaaagaatca ctataaacaa
     2281 ttggaaaggg tgtttatatt gaaaagagta tatatagatt ttgaaatgaa tatgccaaat
     2341 tctaaatcaa aaagagcagt atttaattct gatataattg caatcggagc agttatgtat
     2401 gatgaaaaaa caaaaaatat tgataaattt aaatctttaa taagaccagt aagtaatgag
     2461 gagttgtatc ctcatataca agaattaaca catatatctt ctgaagaatt gaagagtgca
     2521 ccaagttatg aagaggtcat gagaaagttt aaaaaatggc ttggcatatt ttctgatatt
     2581 aaaggaatat atacttttgg taatttggat ttgacatgtt tcaataacac agatatgaga
     2641 agtgctaaaa aaaataacca tccaaggttt gtaaataaca ttagagattt atttgtagat
     2701 ataaaagaaa aatatataaa ttgtggtatg agatgtatga actatatatc attgaaaaat
     2761 ttacttgaat ttgttaactt agagtttagt ggagatgccc atgacccatt aaatgatgca
     2821 tataatttat ttattttgga tgaggcaatc acaaataatg tagatataca aaatctttta
     2881 ataatacgtg acataataag accaccattt aacgatataa attctaattt agataattgt
     2941 tttaacaaat ttaaggagtc attatataaa aaagaaggaa attataatat tgtagatttt
     3001 tctgttgaaa taataaaaac tgtgagaatg tacttattaa caatcataga tgtaaatata
     3061 caaaatttag aaattatgaa agatataagt aaaaaaatgg atactataga taaattgaaa
     3121 gatatagagg aaggctattt ttatttatta gaagatgtgt acttcgatat gaaaga
//
LOCUS       DACULQ010000267         3162 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.269, whole genome shotgun sequence.
ACCESSION   DACULQ010000267 DACULQ010000000
VERSION     DACULQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.269"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     repeat_region   125..1209
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=191..219
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            1460..1636
                     /locus_tag="JA154_17805"
     CDS             1460..1636
                     /locus_tag="JA154_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAT7997682.1"
                     /translation="MLTILSILIILLLAGTNIKIISERLGHTNIKITMNRYFLVLNDM
                     DKKASENIIKVLFK"
     gene            1856..3127
                     /locus_tag="JA154_17810"
     CDS             1856..3127
                     /locus_tag="JA154_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HAT7997683.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1251 a          418 c          402 g         1091 t
ORIGIN      
        1 aatcattaaa actaaagtca atgttatctg gtaattattt actacaaatg ctaaaaaata
       61 attatatttt aaaggataaa atgctgtata aattattggg gtattttacc tatacttgtt
      121 gcaaatttac ataccacgta gttaatataa aacaagatgt ttttaaaaat aaacttaata
      181 tagatattga atttacatac cacatagtta atataaaact ttaatcatat atcagatagg
      241 tcacaactta agagtgattt acataccaca tagttaatat aaaacaactt ctcatattcc
      301 agataattgt tattttacga aatttacata ccacatagtt aatataaaac ctgtagattt
      361 gcctgtttcg atattgctca tgtgtgaatt tacataccac atagttaata taaaactctc
      421 ttgtttcttc tgttaatttt attatacctt cattatttac ataccacata gttaatataa
      481 aacgcgttat attgttgtat aaaaccgatt aaagttcaat ttacatacca catagttaat
      541 ataaaacttg caacatattg ttttatcgtt gctgggtctt taaaaccatt tacataccac
      601 atagttaata taaaactaag cgatattaga gagcgcaagg catagcggag caaatttaca
      661 taccacatag ttaatataaa actttaattt taaccacccc ctttcactaa taaaaaaaga
      721 tttacatacc acatagttaa tataaaacgt tgcatatgta tgtctaagag catgaaattt
      781 aattttattt acataccaca tagttaatat aaaacttcta ttaagtgtaa gcaaaacgct
      841 tggaatatga aatttacata ccacatagtt aatataaaac ttatacacaa atacaacttg
      901 aagaagcgct aaagagattt acataccaca tagttaatat aaaacctaat gagtacatag
      961 ataaacgtac aggagaggtt aatttacata ccacatagtt aatataaaac gttgataatt
     1021 ttcgtttgta tactttttat aatttaaaat ttacatacca catagttaat ataaaacata
     1081 aagatttaga aaaagaaaaa gttaaaattt tggaatttac ataccacata gttaatataa
     1141 aacttttcct gttaagttga tgtttgcaac tttaactgct atttacatac cacatagtta
     1201 atataaaacc ccaaaataaa ctaagtattt tcagtactta cacacataca actctctcaa
     1261 atttgcagtg aaccatgagt agtgcaattg ataacattta tcacacaccc tcaatccctt
     1321 acattgcaac tgttaaaccc tactttatca aaaatatcgc tcactgcaaa atttatatat
     1381 ttttattata tcataaaatt caaactataa aattaaaatg gaataattta tcaatttaag
     1441 aacacctaca tatttgtaaa tacttactat attatctatt ctaataattc ttcttctagc
     1501 tggaactaat ataaaaatca tctctgaaag attaggacat actaatataa aaataactat
     1561 gaatagatat tttcttgttt taaacgatat ggataaaaaa gcttctgaaa atataatcaa
     1621 agttctcttt aaataacatt ccattttatg taagtaggca ttatattttt cttaaatgtc
     1681 acttaaatgt cagttatata taataatagt aaacaactgt atttcactaa tatttgctat
     1741 tatctaatag tatttatagt ttagatttga tataatacat gtggtaaaat tttgatttgt
     1801 aaaacaaaat tttacctaca tatattaaaa caaatttaaa actaaggaga cctatatgtt
     1861 aaaagtatta aaaggaaata taatttttac aaaaacttca gaaactttca cagtatttga
     1921 aaatagttat attatactgg aaaaaggtaa agtaaaggga atatttaaag agatacctaa
     1981 ggaatacaaa aacctaaaag tagtagatta cactgataag cttataatac ctggtatgaa
     2041 tgacctccat gcccatgcct ctcaatttaa aaatcttgga atggctatgg ataaagagct
     2101 tttgccatgg cttgaaactt atacatttcc agaagaatca aattataaag atatagaata
     2161 tgcaacaaaa atgtataaaa aatttgtaaa agaactttgg aaatctggaa ctacaagaat
     2221 agcagtgttt gcaacagtac ataaagaagc ctctatgaaa cttatggata tattcaaaga
     2281 agttggtctt ggagcatacg ttggtaaagt aaatatgaat atgaattgcc ctgatacttt
     2341 attagaaaac actcaggatt ctattactga tacagaagaa attataaata agtatagtga
     2401 tacagattct attgtaaaac caataataac acctagattt ataccaagct gtacaagtga
     2461 acttttaaaa ggtttaggag atttatgttt aaaatataat attcctattc aatcacactt
     2521 atctgaaaat tatgatgaga ttgaatgggt tagaagtcta gaacctgaat ctaaattcta
     2581 tggagatgca tataatagat ataatttatt tggtcaaact cctactttaa tggctcattg
     2641 tgttcattgt tctcaggatg aaatagattt aatgagagaa aataatgtta tggcagttca
     2701 ctgtccagct tctaacttta atgttggtag tggtgctatg cctatcagaa aattaatcga
     2761 taataatatt agattagcac ttggatcaga tataagcggt ggacatacat tgtcaatatt
     2821 taaagcaatg gtatctgcaa tacaattatc taaattatat tgggttaatt ctggtaagaa
     2881 gtataatttc ttatctttat ctgaggcttt ttatattgca accaaaagtg gtggaagttt
     2941 ctttggaaaa gttggtagct ttgaagaagg ttatgatttt gatgcattga ttattgatga
     3001 ttcaaattta aatcatgaca attattctat acttgaaaga cttgaaagat ttatctatgt
     3061 tggtgatgat agaaatatca ttcatagata tgtatgtgga aaactaattg aagaacctaa
     3121 tatataatta taataaaaat tttaattata tattatggtt aa
//
LOCUS       DACULQ010000268         3140 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.270, whole genome shotgun sequence.
ACCESSION   DACULQ010000268 DACULQ010000000
VERSION     DACULQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.270"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1898)
                     /locus_tag="JA154_17815"
     CDS             complement(<1..1898)
                     /locus_tag="JA154_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAT7997684.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSMKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLI"
     gene            complement(1921..>3140)
                     /locus_tag="JA154_17820"
     CDS             complement(1921..>3140)
                     /locus_tag="JA154_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT7997685.1"
                     /translation="SLFSIWSLLLINIILAMGGYIFYFKNFDKEIKEIDEYPMISILV
                     PAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILENIKDRYNNYNFTII
                     NTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLVQTIVMNDELGAVI
                     GKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTNFILRRSIIEEIGG
                     WDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQRTRWAKGNIYVLM
                     KYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISKLVEISIPINFFLI
                     WILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFVAIKGMIEYLKDII
                     FKREVKWYKTERF"
BASE COUNT         1047 a          499 c          297 g         1297 t
ORIGIN      
        1 attatcaatg tatctccttt taaatcatta actttacttt tattcaacaa ataatccatt
       61 cctaaatata agtttttttc attcattgaa ctaattacca tgatattttt ttgattatta
      121 tatggtgatt ttattaattg aatggtagaa atattttttc cataatcatc aataaagctt
      181 atcttatcat ttgatataaa attggagtaa tttttatcga attttatatt taaattatta
      241 tttaacatct ttataacaga attattatgt ggcactccaa aaactacaat attagtatcc
      301 ttatacttgt cactaaattc ttttcctcta attacattta tatttccatt atgtgaattt
      361 atattcgcac ctaaagtaac acctaatcta aacatccaag tcatagcttg tgaccctgaa
      421 taatctggca ttatcacagt taaatcatta aattcatcat ctctaacaaa tggatacgga
      481 taattttcaa aagataaggt ttcattttct ttcatagata acgccaaata agaattattt
      541 gatacatatg cccaaggatt attactctct ctagtaacac aattcgaatt ttttatactc
      601 aaattaaatg tcaatttaac ttgataatag tttttattat ctatatcttt aggtattttt
      661 aattctaact tatcattatt agaatgtgac ctatccaatt ttttacttcc cacaataaca
      721 tcatttatag acactgtaac taaagatttt tcaaaatcta aattgtcaga atatcttaaa
      781 tttaagataa tctttgaacc atcatctaat accttacccg ttggtatttt gacatcaaac
      841 aaagcttgtt gattaaatgc tccttccaac aaaaagtcag aatatcctaa gtcttttaat
      901 gttaaatgac ctaaatttaa tttttgttcc ctattaattt ttatattagt ctctttgttt
      961 acaagtactg atgatgataa aacttggtta gaaagtctat tttctattaa taaatttgat
     1021 gcctttatta aatcatcttc attactacca attaccacca tcatcttctt attcttattg
     1081 tatggtgaat ctacttgctt gattatacag tttgaagata atagggtttg ttcctttata
     1141 cttaaaatat ccaaaatttc ttctgctgta ttttcaggtt taccaatata tataatatta
     1201 tcatctgacc aatttttcat ttctgaatat aatttaacat ctaactttaa atcatcattt
     1261 tttgttattt ttccaaatgc tgatgccaaa ttaaacactg ctgtagtctc tcctctagtc
     1321 atattatctg gtactactat agttgtatct aatttatgat tattttcaat ttctgcatat
     1381 ggatacggat attcatttat tgagttttcc acctttttct gcttatatct aattgatata
     1441 tatgattctt tgtgaataac catccagttc ccagtattgg aatcatcttg gcaaatttta
     1501 tcagatattg ttttgtatgt ttttattttt atttcattat atccttgaat tatataatct
     1561 tttggaacta atacttctaa tgtattttta taatttgtct tagcatttaa tttaatagat
     1621 tttattggta cattatttat caatacagtt aaacttgaaa catctccatt taatatttgg
     1681 ctcttactaa aattaagatg caataaaact tcttcaatat cccaattttt atttacttca
     1741 aaaaaagtac tattacttcc tataacacca tcaatagtta tatctctttc aaacttataa
     1801 tttttaactt tagtctcatc agcatttact ttgtatataa gtgatatatt tgaaaaaaac
     1861 aatactaaag atataattga tataataaat tttttcatgt taattcctcc tgtacatgaa
     1921 tcaaaatctt tctgttttat accatttaac ctctctttta aaaattatat cttttagata
     1981 ctctatcatt ccttttatag ctacaaacag ccatagttga gagtatgtaa aatacattat
     2041 tgccactatg aatatatttt caattgttgc ttcacctttt tctatagtaa ggcttatact
     2101 cacttctata ataaataaaa gataagatag tatccatatt agaaaaaagt taattggaat
     2161 agaaatttct actagtttag atatacttaa aacaaataaa atatcggata taattacaga
     2221 tgttaaaaat agaaaatata cagaaaaaaa gtaagctata tcaaacacaa tcttatttct
     2281 tccctgttta aatatatttt ttatatattt cattaataca tatatattcc cttttgccca
     2341 tctagtacgt tgctttatcc acaccttaac agtttctggc tcttgttccc atgttataga
     2401 ttgtggtaca agctttatct tgtatcccaa tttatatatc ctaaaactta tttctgtatc
     2461 ttctgcaatt gctttactat cccatcctcc aatttcttct atgatacttc tcctcaatat
     2521 aaagttagtt cctggtattg tacatagatt aaaaagttgc catctaccag cttgtgacat
     2581 ccattgaaag cttaatgttt ctatgtttat aaatttggtt aataggtttt tatttttatt
     2641 tcgagtccta aatttcccaa taacagcacc taattcatca ttcattacta tagtttgtac
     2701 tagatatcta agtgcatttt tatctggtgt gttatctgca tcataaacag ctataaaatc
     2761 tccttttgaa atcgtatatc caatatttaa tgcattagac tttccttttc cacctgttaa
     2821 actatctgta tttattatag tgaaattata attgttgtac ctatccttta tattttctaa
     2881 aatttcttta ctattatcag aagaattgtc atttataaca ataagttcca tcttactctt
     2941 aggataattg agtaatagca aagactccac agttctacct atcactttag cttcattatg
     3001 agcaggaaca agtattgata tcataggata ttcatcaatt tcttttattt ctttatcaaa
     3061 gtttttaaaa taaaatatat atccacccat agctaaaata atatttataa gcaataaaga
     3121 ccatatacta aacagactaa
//
LOCUS       DACULQ010000269         3126 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.271, whole genome shotgun sequence.
ACCESSION   DACULQ010000269 DACULQ010000000
VERSION     DACULQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.271"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1046
                     /locus_tag="JA154_17825"
     CDS             <1..1046
                     /locus_tag="JA154_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAT7997686.1"
                     /translation="EKCLLLENKLPVFMKDYFIYLKGSVAVSTRLAYLEDINFFCSYL
                     IETKELTNADCVKDITEDDFNTIKSRDINLFLGDYCSRYYKNTEKNTLIFENNNRALA
                     RKKSSISTLFKFLYRNSQIDNNITDGFNPIKLPKPQPDAIKRLEIDEVAKMLESVETG
                     EGLTEKEKVYWRKTKLRDKAILALFVTYGLRLNELRELNISSFNFSRGEFKIYRKRGK
                     EVLMPINHTCEHVIKDYLQNERTRDELLNDEEKDALFLSLQNKRITAKAIRTLVKKYT
                     SIPLDTTRDNGYSPHKLRATAATSLIQTGFSIYDVQNLLDHDNVTTTQLYAAHKKNVK
                     RDIVKNFEWIEDD"
     gene            1150..1785
                     /locus_tag="JA154_17830"
     CDS             1150..1785
                     /locus_tag="JA154_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAT7997687.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(1932..3089)
                     /locus_tag="JA154_17835"
     CDS             complement(1932..3089)
                     /locus_tag="JA154_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT7997688.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTLDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFILIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
BASE COUNT         1133 a          456 c          448 g         1089 t
ORIGIN      
        1 tagaaaaatg tctacttttg gaaaataaac ttcctgtatt tatgaaggat tattttatat
       61 atttaaaagg ttctgttgca gtttctacac gacttgctta tttagaagat attaattttt
      121 tttgttcata tttgattgaa accaaagaat taacaaatgc agattgtgta aaagatatta
      181 cagaagatga ttttaataca ataaaatcaa gagatataaa tttattctta ggtgattatt
      241 gcagtagata ttacaaaaat acagaaaaaa acacattgat ttttgaaaat aacaatagag
      301 ctttagcaag aaaaaaatca tccatctcta ctctatttaa attcttatac agaaactctc
      361 agatagataa taatataact gatggattca atccaataaa acttcctaaa cctcagccag
      421 atgctattaa gcgccttgag atagatgaag ttgctaagat gcttgaatca gtagaaacag
      481 gtgaagggct tacagagaaa gaaaaggtat attggagaaa aactaaatta cgtgacaaag
      541 ctatactagc attatttgtt acatacgggc ttagattaaa tgagcttaga gaactcaata
      601 tctcctcttt taatttttca agaggtgaat ttaaaatata tagaaaacgt gggaaagaag
      661 ttttaatgcc tataaatcat acttgtgaac atgtaataaa agattatttg caaaacgaaa
      721 gaacaagaga tgaactatta aatgatgaag aaaaagatgc tttatttttg tctcttcaaa
      781 ataagagaat tacagcaaag gctataagaa cactggtaaa aaaatataca tctattccac
      841 ttgatactac tagagacaat ggatatagtc cccataaatt aagagcaacg gctgcaactt
      901 ctctaatcca aacaggattt tctatatatg atgttcaaaa cttacttgac catgataatg
      961 tcacaactac tcagctttat gcagctcata agaaaaatgt aaaacgtgat atagtcaaga
     1021 attttgaatg gattgaggac gattaaaaat caggaacaaa tgtttgcaga tataaatgga
     1081 ttatgttata attaaaaata taaagtgtat ggaaataaaa acaaaatatg tttggaaatg
     1141 agtgatgtta tgtatttaga tctaactgaa aagcaagtct tgatattgga atttataaag
     1201 tctcaaatca tattaaaagg ttatccacct gctgtaagag aaatatgtac tgctgtaggg
     1261 ttaagatcta cttcaactgt gcactctcat ctaaataaac ttgaaaaact gggatacata
     1321 agaaaggacc ctactaaacc aagagctatt gaagttttag aacgtagtaa agtaaatgat
     1381 gtttctggag ctaatcaaga aataatagag cttcctttag taggtcaaat aacagctgga
     1441 gaacccattt tagctcaaca aaacatagag gaatacattc cattccctgc cagtttagta
     1501 aagggcagta acaattttgt attaagagta aaaggcgaaa gtatgattaa tgcaggaatt
     1561 ttagatgaag attacgttgt agtagacaaa aaaaatacag ccttaaattc tcagatagta
     1621 gttgcactta taaatggtga atctgctaca gttaagaggt tttttaaaga aggaaattta
     1681 ataagacttc agccagaaaa tgattttatg gaaccaatta tgctcaatga ctcagaagtt
     1741 gaaattgtag gtattgtaac tggtgtattt agagttatta aatagctatt aaaaaatact
     1801 taaaaaaata agctattcgt atagataaaa tgtgttatct attgattata gcttattttt
     1861 ttatgcattt attttatttt ttaaatgatt taaagttcag aagctttata gataaattgg
     1921 ttgttttttt gttattttcc ataacatata tcacatgggt catttttctt actttctgca
     1981 attgtgcctg ataatatttt tttacttctt gaaagcccac tacaattttt tgtagagtga
     2041 taactcttac cttttggagt aaaatacact atttcttctg aattggatgt attttgttta
     2101 tttttaaaag aatcattttt acttgtatta ctgtttgatt ttccagagtt tccatcttct
     2161 ctaacgcctt tattataact tccatcttta caacttgtaa atactccatc acctgttgat
     2221 tcaaatatta tatctgaaca ctcatcactt ctatgtactt ttactcccat tttttttaat
     2281 ttattcattg tctcctgatg tggatgacca tatgaattac ccttgcctac taaaagaacc
     2341 gcatattcag gatttacttt atctaaaaac tcttgagatg aactactacg agaaccatga
     2401 tgagctactt ttaaaaggtc tactttctca agcttaggga gtatttcttt ttcggtacct
     2461 tcttcagcat cacctgtaaa taaaacttta tcattcccat tagtatatac aaggactaga
     2521 gattgttcat tagtagtatc tcccccattt gtatttagaa cttcaaaata agaatcttct
     2581 aaatagaact tattattttc aagcggtaca cttggggata aacccttatt tgctaaagca
     2641 tttataaagt ccctataact ctttgtttct gctgaaccat tcgatacaaa aacattttct
     2701 atattaaagt ttttgacaac agaatcaagt ccacctaaat ggtctgcatg agaatgggta
     2761 gctataagat atttaatatc ttttacacct tgattgttta ggtaatctac cattaatccc
     2821 tcatcatcat tatctccccc atctatcaat ataaaagttt taccatcttg aatcaatatt
     2881 gcatcactat ttccagtgtc aataaaatga atttttacat tcttatcact tgatatacta
     2941 ctagacaaag aatcattatt atttgattga ttatttccat tatctaatgt agtacaacca
     3001 gttaaataaa ttgatataga tactatcaat aaaagtatca aagttttaat tttagaattt
     3061 tttttatttt ttatacttat ttctatcaca aactacgcct ccatattcat aatactctag
     3121 taatta
//
LOCUS       DACULQ010000270         3122 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.272, whole genome shotgun sequence.
ACCESSION   DACULQ010000270 DACULQ010000000
VERSION     DACULQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.272"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1091)
                     /locus_tag="JA154_17840"
     CDS             complement(<1..1091)
                     /locus_tag="JA154_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997689.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLL"
     gene            complement(1091..1837)
                     /locus_tag="JA154_17845"
     CDS             complement(1091..1837)
                     /locus_tag="JA154_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacitracin ABC transporter ATP-binding protein"
                     /protein_id="HAT7997690.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            complement(1984..>3122)
                     /locus_tag="JA154_17850"
     CDS             complement(1984..>3122)
                     /locus_tag="JA154_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HAT7997691.1"
                     /translation="AEGNKILIHISQVLNNNTNKYEICARMDNDHFILLIACNTEDEL
                     LNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLLIDRANIAAKSKKE
                     KYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYSLDDVNEIEGAEAL
                     IRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWINKGYTPVPISVNM
                     SRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILIDIMKKIKEIGFLI
                     SMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISKIVEMAKAIDIKVI
                     CEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
BASE COUNT         1098 a          523 c          294 g         1207 t
ORIGIN      
        1 cttagtaaga cttccctatt atatttaagt acaggaaaac ttaactcctt ataatcatat
       61 cctttgattg cttctctaat ttgttgtagt tcttcatatt ctttttcatt attatgctct
      121 gaaaaatatg agtaaaccat tgggaaatta tttttagaag cttctccttg agaacttaca
      181 agtgttgaag ttgttgtaaa tgctactaaa gtagccgata aaagtattgt tattaaaaat
      241 aacaatcgtg tattatcctt taatttatat atcaaattac ttataagtaa catatttact
      301 ttgttcttat aaaatttttc tttatttttt aaatacttta atatcaaaat agtaaattga
      361 gaaaaaaata aaaatatccc caaagtaatt aatagtgctg ttgtatacat acctccattt
      421 atttttaact tattgccaaa tagtatcata gcatatccta atataagaca tattacacca
      481 attattccaa atagctttga agattttatc tcttttttag gcttttttgt acctactaaa
      541 agctctaaaa tgctcttttt actcaataat tttaagctca ttggtcctgt tatcaaaaat
      601 aaaatcataa atgatacaat agtcaatagc attgctttta ctggaaaata aaattcagtt
      661 aaattcatat aaaacagttt gcttaaatac aataaaaaca attttgaaat tgataaacca
      721 agtaatattc ctaacatcac tgctaaaaat cctattatca aattttcaat taatatcaat
      781 tttttcagtt gtctgttact cattccaatt atcatcaata ctccaaaatc tttcaatcta
      841 tattttaaga aatttcctaa agagtacaat ataaaaagta gactaaaacc gaatattatt
      901 aattctgaag tcataagagc cataaatgca gtagaaccac tctgcattcc agattttgtt
      961 ataattggat ggaacataga cactgcaaaa acaaaaaata tcattataga aaaaacagaa
     1021 cttaaaaaat atgctaaata tgccctgaaa tttctaacta tattattata agcaaacttt
     1081 ctaaaatcca ctatcttaca cctcctattt gagacaaaac agctagtata tcagaataga
     1141 aagaattact gctttcagat ttttttattt ctgtatatat acaaccatct tttataaaaa
     1201 ttactctatc tgaataactt gcactatatg cttcatgagt taccataagt gttgttacct
     1261 tttgttcttt gtttatccta gtaaataatt tcaaaacatc atcagtagat tttgaatcaa
     1321 gatttccagt tggctcatct gcaagaagta tggcaggttt atttataata gctctagcaa
     1381 tagcacacct ttgagcttga cctcctgaaa tttcataggt ttttctatct aaaattttat
     1441 ctattcctaa aaatttagat atacttttag tttgtatatt catatctttg atacttgccc
     1501 cctctagtgt aagtggcaac ataatatttt cgccaacagt taaagtgtca atcaaattaa
     1561 aatcctgaaa tacaaaacca agttgtttcc ttctaaattc agcaagtttt tctcctttca
     1621 atttacttac atcatatcca tctaatacaa cctcaccttc actctgtcta tcaactgtag
     1681 atattatatt taaaagagta gatttaccac ttccagatgg tcccataaca gacacaaatt
     1741 ctcccttttc tatatttaga ctaaccttat ctaatgcttt aaatggaata tttccacaat
     1801 atgtttttga tatatttcta atactcaata tgttcatttt atattctcca taatttttta
     1861 tttttactac aataccataa tatcgtgaat atactacttt aaccataaaa ataccctaac
     1921 aattaactta catatttgta agtaagttgt tagggtatct tatagtctaa taattattaa
     1981 tgattaatca aatttaaagt ttaagtgttt ttcaaattca tctaatacca taggtttagc
     2041 aaataagtat ccttgaacct tatcacaacc tatctctttc aagaactcta cttgttcata
     2101 agtttctacg ccctcacaga ttactttaat atcaatcgct tttgccattt ccacaatctt
     2161 agaaataata atttttccac gttttgtatc tgttgtttcg attaaaaatc ctctatctaa
     2221 ttttaaaata tcaaaagaaa ggtcttttaa cagatttaaa gatgaatatc ccgaaccaaa
     2281 atcatccatt gatataagga atcctatctc tttaatcttt ttcattatat caatcaatat
     2341 atttaaatta tcaaacacaa cactttctgt tagttcaagc tcaataaact caggagatat
     2401 attgtattta cttatcaaat cagttatatt ttctataaaa ttatccctat ataaatgaac
     2461 tctagacata tttacagaga ttggtacagg agtataccct ttatttatcc acttgtttaa
     2521 tgttttgcat acttcttcaa acacgaacat atcaatattt acaataaaac cattcttttc
     2581 aaataaaggt acaaaatcta taggtgaaat aaacccaaat tctggactat tccatcttat
     2641 caaggcttct gctccttcaa tttcatttac atcatctaaa ctatacttag gttgataata
     2701 tactataaat tcaccttttt ctaaagcttt attcatatta tcttccaatc gtttttcctt
     2761 gtacaatcga tttcttgtat cttcattaaa aaatgaatat gaatgttcat atttttcctt
     2821 tttacttttt gctgcaatat ttgctctatc aattaacaag tcaatctttt taatatcatc
     2881 ctttttagta atcttatata ttccacttga cataacaatt ttatacttag atgagttagt
     2941 agtatttaag ttacaaattt ctttatttat tttagataat ctatttaaaa gttcatcttc
     3001 agtgttacaa gcaatcaaca atataaaatg gtcattatcc atcctagcac aaatttcata
     3061 tttatttgta ttattattta aaacctgtga tatatgaatt aatatcttat tcccttcagc
     3121 at
//
LOCUS       DACULQ010000271         3069 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.273, whole genome shotgun sequence.
ACCESSION   DACULQ010000271 DACULQ010000000
VERSION     DACULQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.273"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..530)
                     /locus_tag="JA154_17855"
     CDS             complement(<1..530)
                     /locus_tag="JA154_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAT7997692.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNY"
     gene            complement(530..1390)
                     /locus_tag="JA154_17860"
     CDS             complement(530..1390)
                     /locus_tag="JA154_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997693.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFIYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            1525..2325
                     /locus_tag="JA154_17865"
     CDS             1525..2325
                     /locus_tag="JA154_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HAT7997694.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            2350..2919
                     /locus_tag="JA154_17870"
     CDS             2350..2919
                     /locus_tag="JA154_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAT7997695.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         1146 a          452 c          376 g         1095 t
ORIGIN      
        1 ctataattaa aatgctttac ttgatataca atcatactaa tcatcttcaa aagacttcca
       61 tatacaaagt aaattaaaat acttactata aaaccacaaa ctttcatttt tccatatcca
      121 aacttaaaaa ttaaaggcag taaaatcgtc aaaattgcta aaaatatatt tacagaaaaa
      181 aatacatcat ttaaaaccat atttctaaag aacactagtg atgtcaattt acttagtaag
      241 gacacaatta ttgttacaga aattattaat cctacaccta ataaatattt tgagtaaact
      301 atatcttcta tactaactgg catactcctt ataaaaatcc ttgaatcatt ttcataatca
      361 cactcaaacg tatttataaa tatcaaataa ctaacaacaa tacttactgt ataatatgaa
      421 attgaattaa ggaaaaaata aaacacaaaa aatagcaata tgtactttaa attacggata
      481 ccatctgaac taatccctat tattaaatct tttcttatta aattgaacat catctcacct
      541 catttacata ataatacatt agttcttcta tagtaggatt tttgacctta tgcttatcaa
      601 tttctatcaa taggcttttt actttcaagc tatctccaac tttaacaagt gcctcattat
      661 aatatttata ctctttcaca ccaactatgc tttctttaat tgcttctaac tctgctttat
      721 ttgccctaat tatcttatat tcttgttcta ttaattcttt cttaacagta aaaatctgat
      781 ttcctctatt tataaaaaca agataatcag caattttaat taaattatca gtattatgag
      841 tagaaattat tacactacta cttccatttt tcacaaaatc ttgtaagtac tgcattattt
      901 cagttcttac aattgggtct atcccagcag ttggttcatc caaaatcaat aacttagcat
      961 tgtgactaag tgcacttgat aacataagct tttcagattg acctttagat aaattttcaa
     1021 tatgcatatt ttcttctata tcaaacttat taaggtattc atcaaacctt tctctatcaa
     1081 aattcttata aaataaagaa actatttttc taaattcctt aacctttaaa tgcggataaa
     1141 attgtaaaga atcatatata aaaccaatat tgtttttaaa gcttattgga gaattttcaa
     1201 tagtttcatt acaaagttca atttcacctt catctgcatc aataatcccc attataattt
     1261 ttattaaagt cgtttttccc gaaccatttg gtcctataat cccaacaata tccccttcct
     1321 ttaactctaa gtttatatta tttaacttga attttcctaa tttttttgtg atattcctta
     1381 tgctaaacac atcttcacct cttatgcatt tttttatacc cacctataca tattttaaca
     1441 cattcactac aaaggtcatt tataaattta taaattaaga attataaaac tatgttataa
     1501 tacaaagtat gaagaggtga atatatgaaa agaattataa ctataaatac aaaccaatta
     1561 aataagtccc ttgagactaa agaactctta actagaaaac ttatcaatgc tggctttgaa
     1621 gtgtattcag atatttatcc agacacagag cttattatat caattggtgg tgatggctct
     1681 tttttaagaa cagttagaga tttcgatttt cctgagattc ccataatggg aataaataca
     1741 ggtcatctgg ggttttttcc tgatatttta ccagataaga ttgatagctt tattgaagct
     1801 tatacaaaaa aagattatat tattcaagaa atgtcacttc ttaatgcgga agtatataca
     1861 actactagtg gttctaatat gcttgctgta aatgaggttg ttataagagg agacaagtct
     1921 aggactatac acctaaattt aagccttgat aataaacata tacaaaattt tagtggagat
     1981 ggtatgatta tttctacttc cacaggttca acagcctata actattctgc tggaggtagt
     2041 attgttgata taaatcttga acttatgcag attacacctt tacatccgat aaataccaat
     2101 gcatataggt gctttacatc aagtataata tgttcaaatg aatctgttat aaagattgca
     2161 ccagaatata gatttgaaga ttctgtattg attgtcgttg atggagttga gcacagattt
     2221 agacaaatcg aaaatataaa agttagtata tctgatgcaa aaataaaatt acttagaatg
     2281 tcaaactatg agttttggca tagagtatct gaaaaatttt tatagactgt acttaggagg
     2341 atttttaata tgaaaatagg aataatgagt gatactcatg gtagcttact ttactttgaa
     2401 aaagcgttaa atgtattatc tgattgtgat gttttattac atggtggaga tgtcttatat
     2461 catggaccta gaaatgatat tccagaagga tataatccta aaaaatttat tgagacatta
     2521 aataaattag aaaacattgt gattgtaaaa gggaactgtg atgcagatgt tgaccagatg
     2581 gttatagaac atcctattca gagtccttat gtcatgagcc aatttggtga aattagaatt
     2641 atattgaatc atggttatat agaatcagaa gaagaaatta ttgacaaagc aaagaaaatg
     2701 ggtggtgaca tattagtttt aggacatact catgtaaaaa aactatacat ggatgataat
     2761 ttaatagtaa taaatccagg aagtacttct atcccaaaag atggaagcca ttctgtcgca
     2821 attatagata tcataaaaac tgatgatgag gatgagcttg aacttgatat aaacttaatt
     2881 gacatcaata ctggaaatat aataaatatc aatgattagt aaatttttaa taaaaataaa
     2941 ataagcttta atttattaaa ttcataataa agacaagtaa accattttat ttctatctca
     3001 attagaagtt taagtacaat accttaaact tctaattaaa tagaatctgg tttacttgtt
     3061 ttattatat
//
LOCUS       DACULQ010000272         3012 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.274, whole genome shotgun sequence.
ACCESSION   DACULQ010000272 DACULQ010000000
VERSION     DACULQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.274"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..2310
                     /locus_tag="JA154_17875"
     CDS             <1..2310
                     /locus_tag="JA154_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAT7997696.1"
                     /translation="LNKNQLQSRIDDNLINAFDNGKTKTYKQIFKDNIFTLFNLINIV
                     LVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLSVIVSNKVSVIREKEKK
                     EIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGESDIIIKNKGDFLYSGS
                     FVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLNAILKIVGIIIIPLGII
                     LFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAVGTIKLASHKTLVQELY
                     CVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMGNICNSLKDDNATLNAI
                     RDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEFIFKEKNKKLRIEVERY
                     SKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEAKETLEFFYNEGVDIKI
                     ISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDKYSVFGRVSPQQKKQMI
                     LALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNVSNLVLLDSNFASMPKV
                     VMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPFVPIQLSLIGAVTVGIP
                     SFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNIVEYLGYSSSVFSTMCA
                     ILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGGLFSFVALSRELILITM
                     ALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2396..2713)
                     /locus_tag="JA154_17880"
     CDS             complement(2396..2713)
                     /locus_tag="JA154_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997697.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1161 a          335 c          479 g         1037 t
ORIGIN      
        1 ttaaataaaa atcaacttca aagtagaata gatgataatt taattaatgc atttgataat
       61 ggaaaaacca aaacatataa acaaattttt aaagacaata tatttacact ttttaatctc
      121 ataaatattg ttttagtctg tttattagtt tcagttggtt cttttaaaaa taccttattt
      181 atatttattg ctgtaattaa tactattata ggagtaatac aagagataag agctaaaaga
      241 ttattggaca atctatctgt tatagtttca aataaggtat ctgtcataag agaaaaagag
      301 aaaaaagaga ttgatgtaca agaattagta ttagatgata ttatgatttt aaaaacaggt
      361 aatcaaattt gtgcagattc aaaagtttta aatggaaaat tagaggtaaa tgagtctttg
      421 gttacaggtg aatctgatat tattataaaa aataaaggcg attttttata ttctggaagt
      481 tttgtagttt ctggagaagc ttttgtaaga gttgaaaata taggaaaaga taactatgca
      541 aataagatag ctaatgatgc aaagatttct aaaaaacata attctcaact tagaaattca
      601 ttaaatgcaa tattaaaaat tgtaggtata ataataattc ctttaggaat tatattgttt
      661 gcaaaacaac attttggaaa tggagattct atagctacat ctgtagtagg tacagtggca
      721 gctgtaaatg gtatgatacc agagggatta acattactta cgagcattgc tctagctgta
      781 ggaactataa aacttgctag tcataaaact ttagtgcaag aactgtactg tgtagagact
      841 cttgctagag ttgatacact atgtcttgat aagacaggta caattacaga aggtaaaatg
      901 caagttgatg atatagtaat gttggaagaa gtggatatta atgaaataat ggggaatata
      961 tgtaattctc tcaaagatga taatgcaaca ctaaatgcaa taagagataa gtataatgtt
     1021 ttagatacgt ataaagctaa gaatttaata ccattttctt cagaaagaaa atacagtggg
     1081 gttacctttg aaaataaagg tacatattta ttaggtgcat ttgagtttat atttaaggaa
     1141 aaaaataaaa aacttaggat agaagttgaa agatattcta aaaaaggaaa cagagtaata
     1201 gttttagctc atagtgattc ttatatggaa gataataata ttcctgaaga tataaagcca
     1261 ctagctttta tattgatatt ggataaaatt agagatgaag ctaaggaaac tttagaattt
     1321 ttttacaatg aaggtgtaga tataaaaatt atatctggtg acaatcctat aacagtaagt
     1381 gaggtagcta gaaaagcagg tcttaaaaca gctagtaatt ttattgatgc aacaacactt
     1441 aaaaatgata gtgatatata caaagcagtt gataaataca gtgtttttgg aagggtaagc
     1501 ccacaacaga aaaaacaaat gattttagca ttaaaaaaac aaaatcatac agtagctatg
     1561 actggagatg gtgtaaatga tgtactagca cttaaagaag cggattgtag cattgccatg
     1621 gcatctggaa gtgatgttac taagaatgtg tctaatttag tcttattaga ttcaaacttt
     1681 gcatcaatgc caaaggttgt tatggaggga agaaaagtaa ttaataacat tcaaagagca
     1741 tcttctttgt ttttagtaaa gacaattttt tcattcttct tgtcgttatt aacattattc
     1801 ttcttcagta gacagtatcc atttgttcct atacaattat cactcattgg ggctgtaaca
     1861 gtgggtatac catcattctt cttagctctt gaagcaaaca aaagtagaat ttcaggaaat
     1921 tttttactca atatattaaa aaatgcttta ccaggagcac tatgtgtgac agtaaatgta
     1981 attattgttt acaatatagt tgaatattta ggttatagtt caagtgtatt ttccacaatg
     2041 tgtgctatat taactggtgt atgtggtctt ttaatattga gacaacaatg tttgccattt
     2101 aataaagaaa gattattttt aatagtaagt atgactattg catttgttat tgggattcta
     2161 tttttaggtg gattattctc atttgtagca ttgagtagag aattaattct cataactatg
     2221 gcattagcaa tattaatgcc aattatgata aaagttatgt tatttgtctt agatgagata
     2281 ttagagacaa ttgttccaga tttaaattaa ttttaaaatg atataaataa gagcagtttt
     2341 agttgaactg ctctttttag atgtgtttaa ttttatgtaa gaatatgtat ttttattaat
     2401 cagctatata tgagcaacag tagtcaacaa cttcattaac ctttaagtca aaacttgaat
     2461 cactaggaac attaaatttt tgcccttctt tataagttac aaactcattg cttccaggca
     2521 gttttacatc catacttcca gcaagcatct ccattatttc ttcttctcta gtagaaaaag
     2581 tgtattctcc tggcaacatt aatcctaaag tttttctttc tccattaggc agtattatta
     2641 ctctgctact taccttacca tcaaaataaa cgtttgcctt ctttactgca gtaacattct
     2701 taaattcact cattagataa aacctccaaa attatttgta catatatatt atataaaaat
     2761 aatgtaaaaa atgctatata tttacaaaaa aatttagtat ttattaaaaa aattaataag
     2821 aataaaaaag aggtgaaatt gcttaaattg ataaaaaata taaaataaat tgcaaagtaa
     2881 gcaagaaaag aataagttga agagttatat attataaata ttaaaaattt tagtattaat
     2941 aaaaaagata ttacattaat atgaaaatat aggagattag aagtgtttga ttaaaaaata
     3001 gctacacaca ct
//
LOCUS       DACULQ010000273         2991 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.275, whole genome shotgun sequence.
ACCESSION   DACULQ010000273 DACULQ010000000
VERSION     DACULQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.275"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(30..458)
                     /locus_tag="JA154_17885"
                     /pseudo
     CDS             complement(30..458)
                     /locus_tag="JA154_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109553.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
     gene            complement(479..1558)
                     /locus_tag="JA154_17890"
     CDS             complement(479..1558)
                     /locus_tag="JA154_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HAT7997698.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            complement(1612..2901)
                     /locus_tag="JA154_17895"
     CDS             complement(1612..2901)
                     /locus_tag="JA154_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HAT7997699.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
BASE COUNT          974 a          619 c          340 g         1058 t
ORIGIN      
        1 tatcctatct ctacatatag gtttccctat atgctgacta ttttagctac taactcatga
       61 ctaaagtcac gagtgtgcgt agctattttt taatcaaact catatccacc agataccaat
      121 attgaatcta attctatttt ttctcttaag tcttctggcc aatctataaa tggaagaatg
      181 gcagtagtgg ctggctttcc tattcttatc atagccacat tattaagtct ttttctttct
      241 acaatagcat ttgtacaatg agttgtacca agcatagcat attttattaa tgaatggtca
      301 atcttacttt catctataac tttttttaag gcattgtaaa caccagtttc aacatctttt
      361 gtagttgcca ccttaactga atgaattact tttaattccc catccagtat acaagcatct
      421 gtatttgttc ctccaacgtc aattcctaca cgatacatca aaatttcctc ctaaactttt
      481 attttccttg taattcttct actgttttat attccacatc atatccaaag tatccaggtc
      541 caactgtctc aattcctttt tctgttctcc atttttcatt acaaggaaaa gctactacca
      601 taacacgaga gccatatttt attctctctg tagttattgg aaatccagtt tctaaatcta
      661 atactgctat taaatctggt gtaatacaaa gtggtgtatc accttttttg gcaattaaat
      721 tctcattttg gaataatata gaatactctc catcatactc attaatccca gcaaaaaagg
      781 catgtcctct agtaaatcca cctttcaact ctctcttaat atcaattact tttccttcaa
      841 ataacttgta tccacgcaat tcttttagta tattctcaac agcacctttt gaatcagatt
      901 tattttcaag taaaaaccct cctatagttt cagctattgt aagagtatta cctatagcag
      961 cttttttaac ttgagctcca cttaaattat agtcactaat agaagaactt cctcccattt
     1021 ctacagttaa agttctagct aatctttctg accaaattcc atccacagga taaaggatgg
     1081 cagaattacc tttttcatca gacattacgg caggagatgc ctcatatcct tcaagataaa
     1141 aagttaccat ttgagcttca gggaaagctc tacccataga atctgcatct aagataggaa
     1201 gtcctttttt agcagcaact gctataggca taagtgagtt tatccctcct atttctatag
     1261 gaattataac atccactttt ctatttaata atttctccat tatttctaaa ggggtagtta
     1321 actcaacttc tgaaagtaat ttttctattg taattacagg agaacccata ccagatactg
     1381 gaactaccaa agcatcatca tcaagttcat ctaaacttat tacttttaca ggaccatact
     1441 ttttaatggc ttgttttgca accaatttac ctacatatgg gtcacctcca cctccagttc
     1501 caagcactgc tgcaccaact gctattttat ctacactttc ttctgttaaa aatctcatgt
     1561 tactttctcc tcatatttct aatttatttt atactttata aacaaatttc tttatactgc
     1621 tttgtctatt tcttgcttat tttttgttga acaaagcact tttccaataa tagattgtac
     1681 tacaaaccct gctataaaac catctaaagg tgctatagta gtaagagtta taaatccata
     1741 tgttgaaagg taagttatta atataccaat tatccaagac actattgaac gtttaactat
     1801 tggtttatat aatactcctt tatcaaagcc tttaagttct tgctttacta tgtaatattc
     1861 tgctgtatat acaccaccaa caggtgcaat tacaattcca agtaaattta aaaatcctat
     1921 aaaaccttca tatattccaa atacagccaa taatgttgct ataataccaa ggactatagt
     1981 taatactttc tcaggagcct tcttaagtac aagagctata cttaatgatg ctgaatatac
     2041 attacttgta tttgtagtcc attgtgctaa tgtaagtact attatacctg gtatagcaat
     2101 tcctaatgta ataaaaattc tcataatatc tgaagttccc atacatttta ctaatactat
     2161 cgaaactatt atcataaaag catttccaaa ttgtattccc aaaaagcttg aaagagcaca
     2221 gtctcttcta cttttagccc aacgacttat atcaggagat acaattgcac caactattat
     2281 tatactcatt gacagagaaa ctccacctgc aaatgtcata gtagatttca tattatcatc
     2341 aactgaaatt ccatttgctc taaaagctaa gaatatagtt agcatcataa gaactaataa
     2401 aaatggtact gaatacacac tcaacttctc cattgcctta tatccataaa ttgcagtact
     2461 catcatcaag agaccaccta ttaggcttaa aatctgcata cttacatcta aattaaatat
     2521 atcttttatt attatctgtg cattttgagc aaagaaacct acttgaactc caaaccaacc
     2581 taatagagaa acccctataa ctagagaaaa tatttttgct ccagcttctc caaatacata
     2641 ctttgttatc atagatgtag ataaacttgt agatgagcca accaaagagc atgtagtata
     2701 caaaattgca gaaaaaaatg aaccaacaat tactgacaat attgcttgtt tcattggcat
     2761 actctgtgcc aactcagcgc ctaaaataac agatgataaa tccacaccta ttgctactaa
     2821 aactgaaaac atagctaccc aaccacgttt ttgattctct ggaatctgtt ccagagcata
     2881 gttaattttt tcttcttcca ttttcatccc tccatttaaa tcgtaaaact actgacctta
     2941 gcatatttta caattttcta atttataaat ttattaacta tcatattttt a
//
LOCUS       DACULQ010000274         2927 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.276, whole genome shotgun sequence.
ACCESSION   DACULQ010000274 DACULQ010000000
VERSION     DACULQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.276"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..799)
                     /locus_tag="JA154_17900"
     CDS             complement(<1..799)
                     /locus_tag="JA154_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT7997700.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKM"
     gene            complement(849..1310)
                     /locus_tag="JA154_17905"
     CDS             complement(849..1310)
                     /locus_tag="JA154_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997701.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(1362..1817)
                     /locus_tag="JA154_17910"
     CDS             complement(1362..1817)
                     /locus_tag="JA154_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAT7997702.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(1789..2052)
                     /locus_tag="JA154_17915"
     CDS             complement(1789..2052)
                     /locus_tag="JA154_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997703.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(2149..2865)
                     /locus_tag="JA154_17920"
     CDS             complement(2149..2865)
                     /locus_tag="JA154_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997704.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
BASE COUNT         1007 a          483 c          276 g         1161 t
ORIGIN      
        1 ccattttata caattgattt ttatactccc tattaagtga acctgcacta tatagtattg
       61 ataatttatc aaataattca ggaaactctt ttttaacaaa gtcaaaaaaa acacctctag
      121 ttatacccct taagtataaa gtcccaggta aaacataatg tacattacat tcttttgcat
      181 gtctaaatag cgaatttata ttatcaaaat catctgtaat atatggtata attggcataa
      241 tatgtagacc aactgatgcg ttagtttttc taaattcttt taacatttta aatcgtctca
      301 ttgaatccac accatttggc tcaataagcc tttgtgtatc ttcatttact gtggtaattg
      361 tagatgctac attgatatat gtaatcctag atagcttatc aattaaatcg taatctctta
      421 aaattaaatc tgactttgta gaaataatag ctggtgtctt gtattttatt agcaggttta
      481 atatttctgg cataatttta tagtcagctt ctattggttg ataactatct gtaactcctc
      541 ctatattgat tacttctttc ttccatttac tgcttttaag ttgtctctca agtttttcta
      601 ctatatttgt ttttacataa atctcttcaa aataattatt ggagttgatg tacttatgag
      661 agtaaatagc ataacaatat ttacatccat gctcacatcc acgataaata tttaaatccc
      721 atttatatgg catactcctt tttaatttat tacaagctat ttcacaattt attggaatat
      781 attttatttt attatacaaa taatcacctc tttatataca ataatactat aatgttaatc
      841 tatagtaatt aaaacatgat atttccagac cacaattcat aatctttact atatttacaa
      901 agtaatatat tccattcatt tattggtatt cctccacatg ttgctttttc ttctaaatta
      961 tataagcatt gctttaaata cattttatta tgccaatcat catataaatt ttctaaattt
     1021 aaactatgtt cattagatat atcatttgca aaatttctat ttttccattt taatatactt
     1081 gtatatttaa gttcattagg tttataattt ctttttaaca tttcatcatg tacaattttt
     1141 gtataaaatt taaattcatc tatactataa ttcaatactt tattcactaa tagatggttt
     1201 ggagtacctt ttttatctat agaacctttt atagcaagta gctctctcca ttgacttact
     1261 aattgatttt taggtaatac atcaattaaa tccttatgcc ataatctcat aatttaactc
     1321 cttactataa aatattttat aaattcaata accaatctat cttacaattt tacatgttca
     1381 ataattttat ctgtaaaaac tataccattt aaatggtcta tctcatgaca aagacatttt
     1441 gccataaaac ctgaaccttt aatttctatt ttctcaccgt attcgtttag agcttgcact
     1501 gtaacttttt ttggtctttt taatttaccc catacttcag gaaaacttaa acatccttca
     1561 acaacaattt gttccccttc ttgttcaata atttcaggat taacaagctt aataagtcct
     1621 tctcctaaat ctataacaac taatcgcttt aatactccta cttggcatgc agctaatcct
     1681 cctccatttg gagtattgta catggtctct gccatgtcat ttaatatttc tcttatctta
     1741 ttatctacgt tttctacata cttactcttt tttcttaaaa tttcatcatc aaatgttctt
     1801 atctttctta tagccataat attctcccct acttatctgt gctcttgatt ttctttcact
     1861 ttcttcaaat aaaaatacac tttcaattgg ttcattaaaa attcttgaaa tatcatatgc
     1921 caaccatatt gaaggttcat ttttaccagt ttctatagca tttatagcct gtctagatgt
     1981 tccaactaat ctccctagtt gttcttgtgt caatcctaat ttttctctta atatctttat
     2041 tttatttttc atatttgctc cattcatgta aggttaactt tacatcttaa atgtaacatt
     2101 tttcaacatt actgtcaagt caaccttaca tttatatatt aaattttctt atttaactat
     2161 ttttttacta aactttttaa ataataataa aatccatatt aatgatataa caattgaaat
     2221 aaacatatag acataattat cttccttaaa tgccttcgat atccaaaaag ttggtaagaa
     2281 gaacagaata tattgtgctt tatttaaaat aaaaaatggt gctaaaatac ccatcataaa
     2341 aacacctgag aatttcgtaa ttaccattcc ttctagttta tttgatgata aagaaatcac
     2401 taatattgat ataataactc cttgtaataa aactaataaa gaaattgata agtttaacaa
     2461 gagagaaatt ttagttagag aaaaaaagat aagtagaatc atagtaataa taaatgctaa
     2521 gatacctggt attccaaatc gtgaaataag ataaccagtt ttaccaagcg gagttactat
     2581 taaatatctt gaaatgctgt catcaatttc tcccaatatg accattgtag aagcaaaaaa
     2641 gaacataaaa ggtgtaataa aagctaaaag taaatcaaac atcaagaaat acggctctaa
     2701 atttaattga taataaaact tgttaagtga tatattctgt attaatggta ttccaaattt
     2761 tataaatact ccacataata ttggagaaac acatactata aaaagcatag catctctctt
     2821 aatttgttca aactcttgtt taaacgcatt tataatcttt atcatagttt tactccacct
     2881 acctttttcc aactgctaca aataaaataa taagttataa aataaat
//
LOCUS       DACULQ010000275         2916 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.277, whole genome shotgun sequence.
ACCESSION   DACULQ010000275 DACULQ010000000
VERSION     DACULQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.277"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..169)
                     /locus_tag="JA154_17925"
     CDS             complement(<1..169)
                     /locus_tag="JA154_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997705.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSK"
     regulatory      complement(223..319)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1116..1517
                     /locus_tag="JA154_17930"
     CDS             1116..1517
                     /locus_tag="JA154_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HAT7997706.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            1523..2581
                     /locus_tag="JA154_17935"
     CDS             1523..2581
                     /locus_tag="JA154_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase"
                     /protein_id="HAT7997707.1"
                     /translation="MKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAVCVN
                     GVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRKAAG
                     KVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCDNVH
                     RKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINCLMG
                     GEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKSFAD
                     IEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSEYLA
                     KKEKKFKNISKINKKIRR"
BASE COUNT         1020 a          374 c          514 g         1008 t
ORIGIN      
        1 acttacttac taataccatc acttgatgca aacaagattc taccacattt tctccgtact
       61 ctagtataaa atcatctagt tgtgtatcta taaaatcaca ataatcatat gaactaccaa
      121 tataagcagt caacatatgt gtacatctat ccattttatc tctaaacata tatattatta
      181 tccccctaaa aaactttcat aaaaacaaaa agaatatctc tttttggcaa ctggctgaca
      241 taactcatag tttattttat aagcgttagt ccctatagct ttgcgtcact aaatttctct
      301 agttttgccg atttagtttt attctacaat tacaataata caatagaatt gcttattatt
      361 caatatactt atttgaaaat taaataaatt tacaaatatg aacgttattt tatatcaaaa
      421 aaaatagact atgcatgtag aatatatgga aaaacttgat tgtaggtttt gtatgaaaag
      481 acagttgtta ttaatttaag tgagataaat tgagcaacag gcataaaatg agaaaacaaa
      541 taatagcagt taatttttat tattaatact aggtgaacat taaatttcaa aataccacta
      601 gattttatag aataagaaaa tgagacaaac ataaatatat gaatatatta aattatactt
      661 gaacatactt atattttatg ataaaatttc aataataatt attagtgaag gagtgtcttg
      721 cctgatgtca gagttatata taatggtaac caacaaataa atagttggag acgcatatgg
      781 gttctgttta atcagagttt ttaagtgtat ttttgataca tttgattgtt gtgtcttact
      841 ctgcctttat agataactaa cattagacaa caaataataa tattacatat tactttgtta
      901 aattgtggtg tagtgggctt taatctgcta cacttttatt aattttacac agtattattt
      961 tgaatataat tatgttgaaa tagaagatac taaattttct agtccacagg ggttttctgt
     1021 ttttgtgtag gtaaatatgt ataccacata aaaaggcaga tataaatacc tgtggacttt
     1081 ttttgtagaa aatacacgat acggaggaaa taaaaatgaa ttataaaaat agaaaacgag
     1141 cttattatca ggaaaattcc tgtatggata actttatttg caaaaattgt ggttgccttg
     1201 tagttcctga aggagctggt acacaacata gaaatcattg tccacattgt ttacatagtc
     1261 ttcatgtaga tatcattcct ggtgaccgtg ctgcagattg tgatgctaat atggaaccta
     1321 ttggagtgtg ggtgagaaaa aatggtgagt gggcaattat tcatcgttgt acacgctgtg
     1381 gacatcttag ttctaatcgt attgcagctg atgataattc agtgaaatta atgtcaattg
     1441 cattaaaacc actggcacag ccccctttcc cactagaaag aatcattgaa aagttggaaa
     1501 aggaggaaac agaatgaatg gtatgaaaca aatcaattta caagactatg gtctaaatag
     1561 aaattttatt actcaaagta tggaatatca tgatttatac cttgctcgga ttatctcgca
     1621 ggaaaagaac ttttatcaag ctgtatgtgt aaatggtgta ctgtttgcag aggtttcagg
     1681 aaagctgcgt tttgaggcta ctatacaatc tgattttccc acagttggtg atttcgttat
     1741 gttggatcgt tgcaataata aaaatggtaa tgctattatt catcatgtgc ttcatagaaa
     1801 aagcttgttt gtgcgtaagg ctgctggaaa ggtaaatgag gagcaggtgg ttgcagctaa
     1861 tattgacatt gtttttttgt gcatgtcact gaataaggat tttaacattc atagattgga
     1921 gaggtatctg tctttaacat gggaaagtgg agctatccca gtagtagtac ttactaaatc
     1981 agacttatgt gacaatgtgc atcgtaaatt acaagtagtt aattctatag ccttgggtgt
     2041 agatgtagtt gttacctcat ctatagagcc agatggttat aaacagattt ctaagtatat
     2101 taaaccaggc tgtactgttg ccttacttgg ttcttcagga gttggaaaat cttcattaat
     2161 taattgtctg atgggtggag aatttttaaa tacaaaggaa attcgcatag atgatgataa
     2221 aggaagacat gccacgacta gacgagagct atttttaatt tcaggtggtg gtattgttat
     2281 tgacacacca ggtatgagag agttgggaat gtgggatgtt tcagaaggtt tagataaaag
     2341 ttttgcagat atagaagctt atttcggaaa atgtcatttt cgtaattgta ctcacacctc
     2401 agaaccaggc tgtgctatct gggaagctat tgctcatcaa gaactttcgc ctaaaagatg
     2461 ggaatcctat cgtaggctta aaactgaagc agtatttgca gacaataaaa gcgaatactt
     2521 agcaaaaaaa gaaaagaaat ttaaaaatat ctcaaaaata aataagaaga ttagaagata
     2581 agtgtagttt ttttgttcat tctatgagtc tatatactat taatatatta aatttgccat
     2641 caatttgtta ctttaatgtg attttgatgg caaatttggt ttgtggtatt atttaaagca
     2701 atttattaat ttttttaata aagtttgatt ttttatatga tgatatatac atgtgataat
     2761 gtatacatat ttgttacgat aggctccttt ataaagaggt cttatgtaag ataagaaaag
     2821 gcatatttat actaatacat aatagagtat atgcttatgt atgatagaga tgtttcttaa
     2881 gagcaaaact gcaatattgc taatttataa agtaaa
//
LOCUS       DACULQ010000276         2895 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.278, whole genome shotgun sequence.
ACCESSION   DACULQ010000276 DACULQ010000000
VERSION     DACULQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.278"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..129
                     /locus_tag="JA154_17940"
     CDS             <1..129
                     /locus_tag="JA154_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HAT7997708.1"
                     /translation="FIPYTIDYSLEIEEKIKEKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(282..1343)
                     /locus_tag="JA154_17945"
     CDS             complement(282..1343)
                     /locus_tag="JA154_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HAT7997709.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            1617..2357
                     /locus_tag="JA154_17950"
     CDS             1617..2357
                     /locus_tag="JA154_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase"
                     /protein_id="HAT7997710.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            2369..>2895
                     /locus_tag="JA154_17955"
     CDS             2369..>2895
                     /locus_tag="JA154_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein BlgG"
                     /protein_id="HAT7997711.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFL"
BASE COUNT         1214 a          269 c          444 g          968 t
ORIGIN      
        1 tttattccat atacaataga ttattcatta gagattgaag aaaaaataaa agaaaaattt
       61 aaaaatatag atgttttaaa taatgaaaat gaatataaag aaatgattga tattatttgt
      121 gcagagtaga aaaaaaggga gtgtcttaga atagactttt tagtttatga ggcactcttt
      181 ttgtataaaa tcaaatgtat tttcatcatt ttaataatta aaaagagctt atccctattt
      241 gagaaagccc tttttaattt aataaaaatg tcaaggttaa actatttaaa tgcagtaata
      301 atcccattat catgtaagtt ttttatgatt gtagctgcga tagggaatcc aaataatcca
      361 gctattccaa atagttgagc tccaacaaac atacacatta atgttattaa aggatatagg
      421 ccaatttggt gtcctactac ctttggttct aatatttgtc taacaacggt tataattatg
      481 tatagtatta aaagtcctat tgctaaatta atatttccat taatgagtga gataatcatc
      541 caaggtgtca aaatacttcc tgtacccaag actggcaaaa tatcaacaag tgctatgatg
      601 acagatatag taaatgcatt ttcaaccttt aggatagtaa gtccaataag tagttctatg
      661 aatgtcactg atagtagtat tgcataggct tttagtagtt ttaaaagagc attaatactg
      721 ctatttttta tatttattat cataaattga cttttttctg gaaattgttt taagataaag
      781 tttactattt tttgatagtc aaaagtaaag aaaaaagaag caattaaagt aataataagt
      841 tttaacataa atgcaggtgt tttacttgca atactagcaa tagcattgag tgcatttgtt
      901 gaaatagaag ctactaaaga catcatagat tgacttatat tatcccaacc aagtattaat
      961 gctaggttaa cattaggtgt actagcatct atctttgaaa ataaagtatt tagcgttggt
     1021 tgtatacttg atttatatat ttcaggaagt ctaaaaaata catcacttac acttgcaaat
     1081 atcttcacac caaagctaac taataaaaat atacacaacc cataaaaaat taaaagtatt
     1141 actatagaaa taagtgtacg atttaaattt gtattatttg taataagtct tataataggt
     1201 cttaagatgg atgcaatgat aaaactgatt acaaaaggca ttaataatgg caataagaat
     1261 tttaatacaa tatatacaat acttgttatt aaaactacat atgtaaattt aattataaaa
     1321 ttcttttgct tttgaatatc cataatataa aacctccaat gtatttgata ttattctaaa
     1381 tatggtcttt tttacagaaa atatactact gtatattgat tataaactat aaagcttatg
     1441 taaagtaaat aaattatgta aaatttaggt taattttgta taaagagata cttttatatt
     1501 ttacaattaa taaccaaata ctaaaaaata ttttaaataa agaacttaaa attgtgataa
     1561 aattaattta tagatattaa aaaaagtata atatatggtt tttaagaggt gctaagatga
     1621 aaaattttag agatttaggt gggaacaaaa ctgaggatgg cagaactgta aagaagggat
     1681 tattttatag gtctgcaaaa ttatcaaatc taagtgaaaa tgatataaag attttaaaag
     1741 agttaaatat taaatatata tttgattata gaagtgatga agaagctcgt aaacatccat
     1801 caacaataat atcaaatata aaaaatataa gaataccagc aatgagagag ctagaagaat
     1861 caggaggaag ttttggttct attgaagata tgattgatgg attgtttgaa aaagatggtg
     1921 catttaatat gttaaataat agttactata atttgcctat aaataatccg tcatataaaa
     1981 aattagtaga acttataaga gactattcta atttaccaat attaaatcat tgtacagctg
     2041 gtaaggatag aactggtgtg gggagtgcta taattcttat gattttaggg gtatcgagag
     2101 agaatataat gaaagattat ctaaaaagta atgattttgc agataaagag atagaaagat
     2161 ttatagaata taagcctaaa tttaaggaca ttccaaaaga aaatttgaaa tatatatttg
     2221 gtgtaaatga agagtatatg aaaactgcat ttagaagaat tgatgaagaa tacattagtg
     2281 tagaagcata tttatatgga gaatttaatt taaataaaga agaaataaga aaattaagaa
     2341 atcaatatct agagtaaaag gtgagtttat gagtttaaat ataacagcaa gacagataaa
     2401 tataataaaa atattgttaa attcaaaaga tacaattagt ggtattgcct taagtcaaga
     2461 gataggatgt tcttcaaaaa ctatacaaaa tgaaataaag gatattaata aacaacttag
     2521 aaatggtaaa attttgtcaa taagaggagt tggttataaa ctagaaggaa gttttgatga
     2581 ggtaaatcta aatagcaatc tctatgatga tgtagataga gtagaatata taatcagaaa
     2641 aatattaacc ttaagtaatg aagaaaaaaa tactataaaa cttgaagatt tagccgattc
     2701 tatgtatgta agtgtttcaa cagtaaaaaa tgatttaaaa gaagtaaaaa gaatgttgga
     2761 aaagtattca atatcagtta aatctaagca taaacaaggt atatgtgttt tagaaagtga
     2821 agataagata ttaaattgta tagttgactt aagcaataag agagataatc aattgagttt
     2881 aaatgatttt ttaaa
//
LOCUS       DACULQ010000277         2883 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.279, whole genome shotgun sequence.
ACCESSION   DACULQ010000277 DACULQ010000000
VERSION     DACULQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.279"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(279..467)
                     /locus_tag="JA154_17960"
                     /pseudo
     CDS             complement(279..467)
                     /locus_tag="JA154_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424164.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(499..1254)
                     /locus_tag="JA154_17965"
     CDS             complement(499..1254)
                     /locus_tag="JA154_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997712.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
     gene            complement(1430..2548)
                     /locus_tag="JA154_17970"
     CDS             complement(1430..2548)
                     /locus_tag="JA154_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT7997713.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKTGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTDVDIEAFENTNNHIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNM
                     IKNHKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKN
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            complement(2550..2648)
                     /locus_tag="JA154_17975"
                     /pseudo
     CDS             complement(2550..2648)
                     /locus_tag="JA154_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          933 a          470 c          261 g         1219 t
ORIGIN      
        1 tataaatcta caaaacgtat tcctagtaac ttatattttt tgatatctac aatcataaga
       61 ctatattatt ggattcatac ctcaaaaata ttgccttctg aacattatta tattcaaagc
      121 tatgttaatt tacaatgata tatcctaaca tgtattgatt aaaaaaaatt aaaacttgct
      181 tttcatctcc tatatctaaa gaagtggtat ttagaataaa ctattgtaat attaaagttg
      241 aaattttcta gtttatttac tctatagcac catattttaa tactattaag taaaaatgta
      301 gttaaaatat atactaatat ccctgaaata agtatttctt ttttatttag cacacttatt
      361 cgtaaggtat tagttattat atttccaaat tatagaataa atattatact tattgctaat
      421 tataatatta atactgttaa gatatttttc ttcatagtta tacccattat ttaatacaat
      481 ttattataaa ttcaattttt aaacttttct tatctcctta tcagaataaa aatccccttt
      541 acttcctttt atttttacta tttttaaact tgccatcttc tctaatctcc aaagtataaa
      601 ccaatctttt acacgcattt catcttcaaa catacagttt gcaaccactt caattaaagg
      661 ttgccatttg tcactgatat ttcttaatat atatttatca aaataagttt cttcaacact
      721 tttaatttcg ttacctacta atgctctaaa aatagaattt tctttaacta acacatccca
      781 tttattttta aattctagtt tttcattaaa gccaatttct agtgtcttat ctaaaaagaa
      841 tggaaaatct tctggagaca tttcactggt aaatgaatat ctaattagag catcttctgt
      901 ctttttttca tctactaatg caatctttat atttttatct tgaaacttac tcactacata
      961 atatactcca cacaaatctt ttgggttatt tgcacaccat attattatat ttctacattt
     1021 tgataatttc ctatcttcta acaaatctgg cttgaataat agtttcggat tattttcaaa
     1081 aacatttcta atcttaaaaa tattttctct taattcgtag ttatttacat caaaaatatt
     1141 acctattgat aaatcatctt caaataatac aattttatta tgtggtatta actttaactt
     1201 cattgcttct tttaataaca aataacaaat atcctcaaag catatatgtg ccataaaatt
     1261 cacctctttt aaaattaaca atttatttta ttataatata taaactgtaa ttctgaacct
     1321 cccatgacta aagtcacagg gttcctgctt cacagaattt tgcatactaa aatatgtctt
     1381 actaattctc cacaggctat ccccgtagtt cctacggttc ttacatacgt tatttatttt
     1441 gaattgttat tagtcttaat ccttctttta atatatttat acttgcattt atatctctat
     1501 cgtgagtttc attacaacta ggacaaatcc attctcttat atttaagttc ttcacttcct
     1561 cgtttttata cccacattta ttgcatattt gtgaacttgc aaagaattta cctaccttta
     1621 caatttgtct accatgccaa ttagccttgt attctaattg acgaataaat tctgaccatg
     1681 acacatcaga aattgagcga gatagtttat gatttttaat catattcttt acttgtaaat
     1741 cttctatgca aataatatca ttctctttta ttaatttagt agatagtttt tgtagaaaat
     1801 cctttctttg attagctata tgttcttgaa gtcttgcaac ttttaatctt gctttatttc
     1861 tatttaaact accgattgtt tttctcgata attctctttg taatttagca agtttgttta
     1921 atgacttctt aagatactta ggattttcta taaattctcc acaacttgag atacaaaatt
     1981 ctttaattcc taaatctaaa cctatatgat tattagtatt ttcaaatgct tcaatatcta
     2041 catcagtaca gcataatgat acataatatc taccacttgg ttctttagat atagtagcat
     2101 taagtattct accttgaggt acttgtttat cccttatctt aaccatacca agtttaggta
     2161 gttttatatg ttgaccacaa tacataatgt ttccatttgt aaagttagtt ttatatgaaa
     2221 atctattaat tttctttgat ttaaatttag gaaatcctgt tttttcttta aagaattttt
     2281 tatatgcatt atctaaatct tttaaggcgt tttgtaatga aaatttgtca ggttccttaa
     2341 gccattttaa ttcttttttt aaattggtta aatcagaact acactgttta taagtaaaag
     2401 tttctttatt gtttttataa acatctattc ttttagcaag atacttatta tatacaaatc
     2461 tacaacatcc aaaagttttg ttaattaact cttgttgttt tttatttgga tacattctaa
     2521 acttataagc tttttccacc actaccacct cactttactt gttttttctg attattaata
     2581 tattttctta tctgctcctc tgtattttct gatacagtag caacgaaata actaggattc
     2641 caaagatgtc tccccataat actggctgtc tatatttgac atactatatg catatcctct
     2701 accatgagta acttccattt ttcataacct atcataatat taaaatatac aattacttaa
     2761 aacaagtatt ttgaagtttt tttatattta tttttaaata acatatataa gaaccgtaag
     2821 tcttaacttt tatcattttt gaggttgtcg ttcacataag ttcgctacta cctatgcagt
     2881 tct
//
LOCUS       DACULQ010000278         2881 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.280, whole genome shotgun sequence.
ACCESSION   DACULQ010000278 DACULQ010000000
VERSION     DACULQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.280"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1375
                     /locus_tag="JA154_17980"
     CDS             <1..1375
                     /locus_tag="JA154_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT7997714.1"
                     /translation="KLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            1378..1776
                     /locus_tag="JA154_17985"
     CDS             1378..1776
                     /locus_tag="JA154_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAT7997715.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            1816..>2881
                     /locus_tag="JA154_17990"
     CDS             1816..>2881
                     /locus_tag="JA154_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7997716.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKA"
BASE COUNT         1193 a          255 c          492 g          941 t
ORIGIN      
        1 taagttgtat gaggataaaa ttaatttagg attgataaat aattcagata agtttataaa
       61 tattactaaa gatgagaact tcctaaaaca tgttcaaaat tatattatca gtaatgaaaa
      121 agaaaactgt gtaatggctt gttttgattt atgtagactc aaacttataa atgatatata
      181 tggttatgag ctaggggatg aagtgttaaa taatatttta agtaacttaa aaaattattt
      241 tggaaaagaa gctatttatg gaaagttaaa aagtgatgtt tttgtattaa taataaagct
      301 tgaaaatagg gaacagaaga ttccttattt agtaaatctt attaagaata aaattgtgat
      361 actaagtcag attgacagtt ttaaaatgga tataaatatt gaggcttcta ttggaattta
      421 taaatttaac aataatgaaa ttgatataaa aaaagctata gataatgctg atatggcaag
      481 gcttaaatca aaaggactaa agcacataga gtatgtagaa tttgataatg ctatggaaga
      541 agaaatacaa tcaattatga aaatagagag agatttattc ttggctatta aaaataaaga
      601 gtttgtaatt cactatcaac ctaaagttga ttcaagtaca ggtaacatta taggttcaga
      661 ggcattgata agatggttgc acccatcatt aggcatggtt ggaccaaata agtttatacc
      721 tatagctgaa aaaaatggtc tgataaacaa tattggtaga tggctaattc aagaagtttt
      781 tattaccatt aacaagtgga tttcagaagg tataaatgtc ttaccagtct caataaatct
      841 ttctagagta gaactatata aaaatgattt agttgatttt tttaaactga tgtttaatac
      901 atataatatt ccaaaagaac ttatagaatt agaaattaca gaaacgacag cactaagaga
      961 tgtaaaattt ataagtgaaa ggctttatga gattaaatct ttaggtatga atatatcatt
     1021 agatgatttt ggagtaggta attcaaattt tatcaacctt aaggggatac cactagatat
     1081 tataaaaatt gataggtcac ttatattgga tatagttaca aatacaaaaa caaagtttat
     1141 ggtgaaagca attgttaacc tttcacatga tttaaacgta actgttatat gtgaaggggt
     1201 agaggatatg catcaggtca aagtcttaag tgaactggga tgtaatttca tacaaggtta
     1261 tgtatttttt aaacctttgg atgagatgaa ttataaaaaa cttcttactg acggaagtta
     1321 tataaatctg aaagatactc ttttagataa atgcatgaca gttgaggagg agtaaggatg
     1381 atttcagaag atgtagatta ttcaataaga atcataagag ctatatttaa taatggagaa
     1441 gataaaccaa tagatgctaa aatgatatta aaaagtgaag atataccaat actaacacta
     1501 tacagactgc ttagaaagct ttgtgcaaac aatattctta cttttaacaa agaagatggc
     1561 aagacataca tgttaaatgt gtcattggat acaataagtc taagaactat tgtagaagta
     1621 attgatggaa aaatatatat aaataaatgt acgagatatg tatctaattg cagtaattgc
     1681 ttaaaaggtt gtaaagtaaa tcgtgaattg ctagaaatag aaaaagaaat ttataaactt
     1741 ttatcgagaa aatctataag acaaatacta gaataaaaaa ttttcagcta tgataataat
     1801 tggaggtttt cggttatgga tgatatatct caagataatt ttctactgtc taaagagtat
     1861 gagaatagct tagatgtaga tactaaaaaa gcaagtggaa tatattatac tcctaaaata
     1921 attgtagatt atattgtgaa aaaaacttta aaaaatcacg atataattaa aaatccatat
     1981 ccacgaatac ttgatatttc ctgtgggtgt ggaaattttc ttttagaagt atatgatatt
     2041 ttatatgact tatttgagga aaatatatat gaattgaaaa agaagtatga tgaaaattat
     2101 tggactgttg ataacataca ccgtcatata ttaaactatt gtatatatgg tgctgatatt
     2161 gatgaaaaag ctataagtat attaaaagat agcttaacaa ataaaaaggt agtaaatgat
     2221 ttagatgaat ctgatataaa gattaatcta ttttgttgtg atagtcttaa aaagaaatgg
     2281 cgatataagt ttgattacat agtgggtaat ccaccctata ttggtcataa aaaacttgaa
     2341 aagaaatata aaaaatttct cctagaaaaa tatagtgaag tttataagga taaagctgac
     2401 ttatattttt gtttttataa aaagattata gatattttaa agcaaggagg gattggcagt
     2461 gttataactc caagatattt tttagaaagt ctatcaggta aagatttaag agaatatatt
     2521 aaatcaaatg tcaatgttca agaaatagtg gattttttag gtgcaaatat atttaaaaat
     2581 ataggggtat ctagttgtat tctaactttt gataagaaaa aaacaaaaga gacatatata
     2641 gatgtattta aaatcaaaaa tgaggatata tgtataaata aatttgaaac attggaagaa
     2701 cttctaaaga gtagcaaatt tgaacatttt aatataaatc agagattgct atctgacgaa
     2761 tggatacttg taaataaaga tgatgaaact ttttataata agatacaaga aaaatgtaaa
     2821 tatagcttgg aagatatagc aataagtttt caaggaataa ttacagggtg tgataaagcg
     2881 t
//
LOCUS       DACULQ010000279         2864 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.281, whole genome shotgun sequence.
ACCESSION   DACULQ010000279 DACULQ010000000
VERSION     DACULQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.281"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            106..300
                     /locus_tag="JA154_17995"
     CDS             106..300
                     /locus_tag="JA154_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997717.1"
                     /translation="MLYLHILQNFELLILSTLLPLLLLLHQLPLLMLLLMLLNFLLLL
                     FLLVLLLLLFFLLLLSLLLL"
     gene            complement(1004..2560)
                     /locus_tag="JA154_18000"
                     /pseudo
     CDS             complement(1004..2560)
                     /locus_tag="JA154_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein CwpV"
BASE COUNT          652 a          555 c          360 g         1297 t
ORIGIN      
        1 cttttcctac tagttttgtt acttctgcta ttttttcttc tgcttttgtc tttgcttctt
       61 ctttagcctg tgatgattct aatgcagtta tctcactttg tacttttgct atatctccat
      121 atcctgcaaa actttgaact tctgattctg tccaccctgc tgcctttgct tcttctattg
      181 catcagttgc cgcttttaat gttgcttttg atgcttctaa attttctgct gttacttttc
      241 ctactagttt tgttacttct gctatttttt cttctgcttt tgtctttgct gcttctttag
      301 cctgtgattc taattcagtt atctcacttt gtacttttgc tatatcttca tatcctacaa
      361 aactttgaat ttctgattct gtccatcctg ctgcctttgc ttcttctatt gcatcagttg
      421 ccgcttttaa tgttactttt gatgcttcta aattttctgc tgttactttt cctactagtt
      481 ttgttacttc tgctattttt tcttctgctt ttgtctttgc ttcttcttta gcctgtgatg
      541 attctaatgc agctatctca ccttgtactg ttgctatatc ttcatatcct gcaaaacttt
      601 gaacttctga ttctgtccat cctgctgcct ttgcttcttc tattgcatca gttgctgctt
      661 ttaatgttac ttttgatgct tctaaattat ctgctgttac ttttcctact agttttgtta
      721 cttctgctat tttttcttct gcttttgtct ttgctgcttc tttagcctgt gattctaatt
      781 cagttatctc actttgtact tttgctatat ctccatatcc tttaaagctt tgaacttctg
      841 attctgtcca ccctgctttc cttgcttctt ctattgcatc agttgctgct tttaatgtta
      901 cttttgatgc ttctaaatta tctgctgtta cttttcctac tagttttgtt acctctgcta
      961 ttttttcttc tgcttttgtc tttgtttttg acttatcgct tggttttgat gaagaacttc
     1021 ctcctccatg tgaagagcct ccacttgatg aacttcctcc tgaagagtta gaacttggac
     1081 tatttccact tattgttgga gaattatctc ccttaacact acttattgta cctttattat
     1141 ctatagtagt ccctttagaa ttatttgtaa ctgtagttat cttaccacca ttagtcactt
     1201 ttacttgact tgctccagat gctactgtta aactaccaat ctcaccttta gaattatttt
     1261 ctatcttggc tccattttta tcattaacct ttaagttagt tatttttcca tagttaacaa
     1321 aagttccatc ttttacatca tctataacta catcatccac ttttgcatag ttatttacat
     1381 ctccattagg catgttgata gttacagtct ttgtatatgt accatttaag tttactgtta
     1441 ctgctttatc agtttgtatt gtaaatgctt ctttaacctc actagttggc ttaaagttta
     1501 taacatcatt agcagtagct ttatctatag ctgattttaa ttcagaaatt gttttagctt
     1561 caactgtttt tacttcttgt aatgacttca tttcattaaa tgctgtctca ttaccattcc
     1621 caccaacttt agttatctta tcaaaactct tagagttaac tagagatttt tgtgaagctg
     1681 ataagttctt tccaactaaa actactggtg attgattttt agcacctaat acacctactg
     1741 ctagtgcatc tataagttgg tcttgtttat tcataccatc tttagttaca taagcatttt
     1801 ttaaatctgt ctttttataa aattctttta ttacttttgc atttgtatca tttctatctg
     1861 ttccacctag tcttgtacta tttggtagtt tatttttaac agcttcagaa attgaactct
     1921 ctccacctac tacataagat tgagttattc cagcatcttt tataaatttg tctgcaactg
     1981 ctgttccatt tttagaatca gcaagtatta ctggcatatt gttttgtgca gctggtgctc
     2041 ctacacttac agcatctgca agtccttttt caccattaac tacagctatt ttcttaacat
     2101 cacttatagc atctaatttc tttgctaatt ctaaggatgt ttgatatctc tcagctccag
     2161 agattctctc aaaagatatt tttaaatctt ttatttcttt ttctatattt gtacttaata
     2221 cagaagttcc acctattaag tatacttttt tagcttttaa tctttgtatt tctgctttcg
     2281 tcttatcatt taatttatct ttacttgtta aaagtattgg agcatttttt gcttttgcaa
     2341 atggtgttgc agatagagca tctgctatag atgagtcgtt tacgattact acctcatcag
     2401 catttttcca tccatcttga cttattttaa cagcagtttc atatctttct tgccctgtta
     2461 aatttgttgc cacagtttgt gcactagcta tgcctgctgt tgaacttcca gctatcattg
     2521 taactgtaag cattgcagca atcttctttg gtattttcat tttattttct tcccccttgt
     2581 ccatttgata ttacttaatt ttttaacttt gttcccgttc taattctaaa ggatacttaa
     2641 tatctaattt taaagaggtt tctaactata atgatttcat aattaatttg taattatttt
     2701 gttacattgc agtcattatg taaggaataa agaaattttc ttattgattg aaaaatttgt
     2761 aaatttatat tttaaggtaa gtttgatttt tatgttaatg aattgttata aaaaataggg
     2821 ttttgtataa aaaaagtagc ctttatgaat taaaaagtgc tact
//
LOCUS       DACULQ010000280         2842 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.282, whole genome shotgun sequence.
ACCESSION   DACULQ010000280 DACULQ010000000
VERSION     DACULQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.282"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..590)
                     /locus_tag="JA154_18005"
     CDS             complement(<1..590)
                     /locus_tag="JA154_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT7997718.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINST"
     gene            complement(621..>2842)
                     /locus_tag="JA154_18010"
     CDS             complement(621..>2842)
                     /locus_tag="JA154_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HAT7997719.1"
                     /translation="YIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNREFMYQH
                     IDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKGIVAYL
                     KGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTIWNGEP
                     NMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHVLQTKQ
                     IEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDLHLFEE
                     NCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIEITENI
                     LIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLSHNLND
                     FKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPMECKEFE
                     KLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDFYNKTE
                     ITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQKYSNF
                     YFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQFYSML
                     PVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFVYKKSL
                     DMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKNLTKI"
BASE COUNT          978 a          407 c          327 g         1130 t
ORIGIN      
        1 gtagaattta ttaattcttt atttaaaagt gaaacaaaat cacataaaat cttgtctcct
       61 acattatgac cttcatagtc atttatcatt ttgaaattat ctaagtcaat tagtacaata
      121 taatctccta taaatatgtc aaatctatca tttaaatatt ttctattata tgctccagtt
      181 aatgggtcag taattgagta gtgaagattt ctcttatttt cttcatagag ttttgttata
      241 tcattggcca tagtaattat gtaggttttt tcatcaacat ctactttata ttctaaaaca
      301 ttccaatagc ttgtagtatc ttttttaaac tctaaaatcc aatttgaaat tttatcataa
      361 ggataatttt tttcccaatc taattgaata ttgtgagaat ccgtaaaaaa tttagcaata
      421 ttatcaggga gtttcttagc tatttctata ttagtcattc caagtggatt atcttgcata
      481 taacttttaa ttaaattttt taaattatca tttataaata taattctgta gttagaatct
      541 cttataatta gaaatcctgg aaaagttctt aaaaaaattt ctttaaacat attaatttct
      601 ccaaaataaa atactttgta ctagattttt gttaaatttt ttattttatt tacatctaca
      661 tacataccct gggcaatcga ccttccatca attgcagaaa ggttacattg tgtatacata
      721 actgtgacaa tttgaccatc tcgttttatg aatctatata aaaatggttc aattttattt
      781 gtgtcaatca tatccaaact ttttttataa acaaagtcta aatcatctgg atgaacaatt
      841 gctcttaaat ttccgcccat ctcaccaaaa aactgttctt ttgtataacc tataatatca
      901 aacatttctt cattcatata agtaataatc atatcaccaa ataaatcgta gcgaattata
      961 cctacaggca acattgaata aaattgtgat aaaatattac tttcatcaag atgtgtattt
     1021 gtcattttag gtattgaata tgattcaaat tcatttatat agccagcagt tttaattaaa
     1081 tccaaatgta attttgctaa gagtaattta ttttctgaaa ttatacaatt tgcactaaag
     1141 tagaaattgc tatatttttg atgttcagaa gcttcatctg ataaaacggc ttctccacta
     1201 attaataatg aatctgattc agtattatgt ggggttatac ttttgtaaag aatagaaaat
     1261 tttttacctt taatatgttt ttcaaaatgc tctattactt taggaaaaga tgtaatttca
     1321 gttttgttgt aaaaatcaaa ccattgaatg tcttcagtga cattagctct tagttttttc
     1381 atatcttgca taataaaaaa tccatcaaat atcttctcta ttaaagattt tcctatatca
     1441 gaatatttta taaacatttt ctctgaatta ttctctttag atagtaattt ttcaaattct
     1501 ttgcactcca tgggttttgc ataataataa ccttgggcga tatcacaatt tatactttta
     1561 ataaattcta catgttgttc tgtttcaaca ccttcacata ctgtaagcat cccaagagct
     1621 cttgcaatgt ttattataga gtacaaaatg gttgtatttt taaaatcatt taaattatga
     1681 gataaaaata tcctatcaat tttgattata tcaaaaggta gattttgcaa cataccaaaa
     1741 gatgaatatc cagaaccaaa atcgtccatt gatacagaaa aaccataaga acgaagttca
     1801 gctagttgtt gaataattgt atttgtattt tctattaata tattttctgt tatttcaatg
     1861 ctaaaatttg atgtagagac gctgtacttt tcagtaatac cttttagcat acttggaaaa
     1921 ttagggttaa aaaaatgaag acgagaaaag ttacaacaaa ttggaacaaa aacatcattg
     1981 ttatcaatcc atcttcttat atttttacag ttctcttcaa ataaatgcaa atctaattcc
     2041 gttatcagac caaactgttc aaataaagga ataaatttat ctggagtaag aagtccttta
     2101 atagggtgtc tccatcttgc aagtgcctca catccaacta tagtattatt ttttatatta
     2161 taaagtggtt ggtaaaatac ttcaatttgt ttagtttgta gaacatgtga tatttctgat
     2221 aagaactcct tatcattttc taatttattg taaaaactat tatctttaaa atcaaataga
     2281 atgtatctat ttttaccttc acttttagcc ttttttaaag cactatatac ttggttgaaa
     2341 agcatcaaag tagtcatgtt tggttcacca ttccagatag tgctgccgat actaaatgta
     2401 acattggagt atatttcttt ttcattagga agttttattt ttaattctcg ggcgctctct
     2461 ataatagttg cacccatatt atttaactct ttatttgata catttggaag tacaattaaa
     2521 aaaccttctc cttttaaata agcgactatt ccctttgatt ctggaataaa gtgcaatagg
     2581 ttttgagtta gagtaattaa taaattttct ccgactaggt atccatatat atcattaaat
     2641 aatttaaagt tatcgatatt taataacatg attcctatat gtatattttt cgaaatacag
     2701 tcatcaatat gttggtacat aaattctctg tttggtattt tagaaaaaaa atctatttca
     2761 tttaaggttt ttaatttaaa atttctacta tctaattcct tagataatac ttctaccttt
     2821 ttatttaaca tttttatata at
//
LOCUS       DACULQ010000281         2831 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.283, whole genome shotgun sequence.
ACCESSION   DACULQ010000281 DACULQ010000000
VERSION     DACULQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.283"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            19..1128
                     /locus_tag="JA154_18015"
     CDS             19..1128
                     /locus_tag="JA154_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAT7997720.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1505..2689)
                     /locus_tag="JA154_18020"
     CDS             complement(1505..2689)
                     /locus_tag="JA154_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997721.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1075 a          438 c          399 g          919 t
ORIGIN      
        1 atgaaaggta agataacaat ggatttcaaa agcttaggga ttagtgaaaa tacaataaat
       61 attctaaaaa aatcaggaat aacaacacct acacctatac aaaaagaaag tattaaacta
      121 ataaaagaag gaaaagatgt aatagcagaa gctcaaactg gaacaggaaa aacactagct
      181 tttttacttc cgatatttga aaatatatca ttagatataa atgatataca agtacttata
      241 ctttctccta caagagaact agctattcaa ataacagaag aagctatgaa acttaaagaa
      301 tctaaagatg taagtattct tgcagcatat ggaggaaaag atattggttc tcaaataaag
      361 aaactaaaag gaaatattca tatgattata gctacaccag gtaggctttt agaccatcta
      421 aatagaaaaa caatagacct tagtaagtta aagacttttg ttttagatga agcagaccaa
      481 atgttgctta tgggatttaa aaatgaagtg gaagctattt taaaagagac ttcaaacaaa
      541 aagcaaacac tatgcttttc tgcaactata aattctcaag taaaaaaatt agcttataga
      601 tacacaaaaa atcctgttgt agtttctatt caaaaagaag aaatcactct taataatata
      661 aaacaagaag tagtagaaac tacagataga aaaaaattag atgccttatg taaggtacta
      721 gatgaagata atccttttat ggcaataata ttttgtagaa ctaagagaag ggttgacaat
      781 cttgaagaag ctcttgctat aagaggatat aattgtcaaa aacttcatag tgatattgct
      841 caatcaaaac gagaaagaat aatgaaatca tttagaaatt tagatataca atacttaata
      901 gctacagatg tagcttctcg tggtcttgat atatctggag taagtcatat atataattac
      961 gatttaccag aaacaccaga agactacatc caccgtattg gtagaactgg tagagctggt
     1021 gaagaaggct atacatgtgc ttttattgac cctaaaaatg aacgaatgtt aagtgaaata
     1081 gagacggcta tagaatctaa aattagtaga agaattatag aattataatt tatagattgc
     1141 ttaaaatacc aatgccacac aaacactgta atatatagaa agctacagaa aacatgtatc
     1201 aatctatact ttgctgttat actattttat attaaagaaa aaagaggcta tccaaagtag
     1261 gattagtctc ttttattgtc aaaataatga aaatatattt gacttcatat aaaaaagagt
     1321 gccttataaa ctaaaagtcc attttgagac accctctttt tatcttttat tcttagtttt
     1381 atattgcttg tttatagttg tttaatatta gattttgcaa ataatcttaa taaacatgca
     1441 gatatattat ctatagtatt cttatctata gtattctctt tagcaatatt ttttgctact
     1501 acatctaatt tacattttca tttaattccg ctggccattt agcttctgga ttttcctttg
     1561 caaacttctt attcttataa tgtcctagtg taaggaatgg aattactata gtgaatagac
     1621 caagataacc acaatatcca tatccatatt taacaatatt acttaatcct gtaagtgata
     1681 taagcattga cactaccatt attgcacaag ctgtaaatac ccttcttttc attcttgaag
     1741 aaaggaaaga aagtgcttta tggtcttcaa atctatttac aagaccaaat atagtagtta
     1801 ctcctgtaga cattaaacaa aggaataatg caacattata aattatgtat atagtatgat
     1861 ttccagattg acctgcaaca taaagagtag ggagtgttgt ttgcccagca gcaataattt
     1921 ctctatacca tcccattagc atagttacag caagtcctaa tgcaattgca ttcataacaa
     1981 atgaaattat cattgcctta gtagcttctt ttgagttctt aagaagtggt cttgaacatc
     2041 ctgcaagtgt aggtataaca accgattgaa aacctgcata aataaaagta ttaagtattg
     2101 gcttgatata gccaggtgtg aaactcgttc tatttgataa tatttcagtt atttctggta
     2161 ctttagcttt tataccaaca ataaaaatag taagagtaca tactagaata gctattgtca
     2221 ttgctgtagc tgctttagat acaagcatag caccaaatat agtaaagaca agaagtacaa
     2281 ctccaataat aaatacagct acaaaataat ttactcctat actttgaaat actgctgctg
     2341 cacctgctat aacagcactt acagctatta aaaccattag atagtaatat atctcccata
     2401 ttatttcaag ttttggatat ggtctccaaa gctcacaaaa taaatcctta taattcctac
     2461 aattgttatt attatacatt atgataactt ctctcatggt agcagtaagt ataatcattg
     2521 ctaaaattgc cataagtggt gctgtccatc catactgcac atagtactga gtttcttgat
     2581 tccctgtagc aaatcctcct cctgcatgtg aactaaaaag aacgcttgca acaccaaaaa
     2641 tggctaacat agagcccgtt acttttgctt tctttacact tttttgcatg aaatcatcct
     2701 ccctaaaatg atattaaaag tttttatttt aacactttat tgataaaaca ataacatttt
     2761 tgctcattaa attcaactat atttttccaa tgactttaca ttttcaactt atttctacct
     2821 ttatattaaa t
//
LOCUS       DACULQ010000282         2812 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.284, whole genome shotgun sequence.
ACCESSION   DACULQ010000282 DACULQ010000000
VERSION     DACULQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.284"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..429
                     /locus_tag="JA154_18025"
     CDS             <1..429
                     /locus_tag="JA154_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HAT7997722.1"
                     /translation="ITEIKNEQIVKNYSRLIKPKGIISSFITELTHISNEMVENEEPL
                     ELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYLRMLDLEPINNYICTVELLKKCKSYKG
                     KNKKLETACAYYNIENINAHRADSDTLATAKLFLKIKDEY"
     gene            557..1573
                     /locus_tag="JA154_18030"
     CDS             557..1573
                     /locus_tag="JA154_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HAT7997723.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            1927..2631
                     /locus_tag="JA154_18035"
     CDS             1927..2631
                     /locus_tag="JA154_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997724.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1183 a          270 c          498 g          861 t
ORIGIN      
        1 ataacagaaa taaaaaatga acaaattgta aaaaactatt caaggttgat aaaacctaag
       61 gggataattt cttcatttat aacagaattg acacatataa gtaatgaaat ggtagaaaat
      121 gaagaaccac tagaattagt tttacctaga tttagaaaat acataggtga tagaactata
      181 attgcacaca atgcgaaatt tgatttaaaa tttttaaatt actatcttag aatgttagat
      241 ttagaaccaa taaacaatta tatatgtaca gtagagttat taaaaaaatg taaaagttat
      301 aaaggaaaaa acaagaaact tgaaacagct tgtgcttatt acaacataga gaatataaat
      361 gctcatagag ctgacagtga tacattagca actgcaaaac tatttttgaa aataaaagat
      421 gaatattaat tataaatata gagaaaatta gtgaaaatat cctttataaa attgaccctt
      481 aagcttacaa ttttgttctt tgatttattt tgtagaacaa tgtataatgt accttatatg
      541 aagaagggag actaaaatgg gttattacaa tgataatgat aataataaaa taagacgtaa
      601 aaaaattagt aacaacaaag atagtaataa aaaatctaaa cattcttata cttcggcaaa
      661 taaaaaaaat aaaagtccta atacaagttc cagtaaaaga atgagtgaaa aagtagattc
      721 acagaatctt ataaaaagag atagagatag ttttacaaga gataatgtaa gacctttaaa
      781 tacagattac aatcaaagag aaagaagaag agtcgagtat aaacaaagag agaaaaaatt
      841 aaaaagtaaa aaaagaaaaa aacaaaaaag gattagaata gttagggcat tattatctat
      901 tatgttagtt gcactaatta tattttcagt ggcaggtgca atatttacag tttcagctat
      961 aaaagggtca ccagaagtta ctaaaaagtt aattaggtct aaatatataa gtagtgaggt
     1021 tgtaagtata aaacaaatgc caaatgattt aaaacatgca atagtttcca tagaagatga
     1081 gagattttat aagcataaag gagtagatgt tatttctcta gttaggtcag tattaaacaa
     1141 tgtgtttaca gatacaacac aaggtgcaag tactatagat atgcaagttt caaaaaacct
     1201 gcttactagt aatgataaaa ctatgaagcg gaaaataagg gatatgtata atgctaagca
     1261 gatgaataag attatgacta aggatgaaat tcttgaagca tatttaaata atatgtatct
     1321 aggtaaaaat gcatatggag ctgcaaaggg agctgaagtc tactttggta aaaaagttag
     1381 tgaattaaat ttagctgagt gtgctatgtt agctggtatg acaaataatc ctgcaaggta
     1441 tattgaccat ggagaggcta agaagagaca agtgattgta ctgtataaga tgcatcagct
     1501 tggttatata actgataagg agtatagaat agcaaaagct gatgacactc catttaaatc
     1561 agaaattgat taaaattgta agaatatagt attttcaata gtttaaacaa acaaaaatgt
     1621 aataaaaaag gtgatttgcc acccatttgc caccataatt ataatctaat ggcaaattgt
     1681 tttaatgtac aaactagata atagaatatt gtcttaaatg ctatattatg ctaaaattta
     1741 attgtatttt gtaaattaaa aaacttataa tttagttttc tagggttccg cattttataa
     1801 tggtcaggtc caagagaaaa cgcgcaattg ttgtgtacac ggaaggataa aagcctggga
     1861 gatatttaag aatatttcta taggcttttt tatttttaca tattttaaat ttgaggaggt
     1921 tttctaatga aaaaatttgt tattgaggat gcattttgga atctatttcc tagtgcaaaa
     1981 attggagttg ttatttgtaa tgatatagat aactctataa aagatgaaga ctataagaaa
     2041 ataattttga agggagaaga agaagcatta aaatacttgg aggactcaga atttagcaac
     2101 aaccaagtga ttaaagtatg gagagaagca tttaaaaaat ttaaaacgaa aaaaggagca
     2161 aggtcttcta ttgaagcatt attaaaaaga gttcataatg gaaataatat aggaactatt
     2221 aaccctttag tggatatcta caattttatc tcattaaaat atgcattacc ttgtggaggg
     2281 gaggatatag ataaatttat aggagatatt aggctaacta gagcaattgg taatgaggag
     2341 tttatcccac tagggacaga tgaaaattca tttccatatg aaggagaaat aatttataaa
     2401 gatgatggtg gagcaatttg tagatgttgg aattggcgtg aagcagtgag aacaatgctt
     2461 actgaagata ctaacaatgc ctttttatgc attgaattag tagatgaaag tagatttaaa
     2521 gaatttgaaa atgctttaaa tgaattagct aaaatagttc aagaaaaatt gggtgggaat
     2581 tgtaaaattt caattttaga tattgataat aaagaggtat cagtggatta atatggaggt
     2641 gctgttgtaa tggactattc tagttataaa gatacttata tttagggtaa gaaggtctat
     2701 tttagatata acgattatca aaggatataa tatagaaaat aaaaatgtag aatataaaaa
     2761 gcctttaaaa taagaaaaaa atgtatttta aaggctttta ttatactctt tt
//
LOCUS       DACULQ010000283         2772 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.285, whole genome shotgun sequence.
ACCESSION   DACULQ010000283 DACULQ010000000
VERSION     DACULQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.285"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(10..708)
                     /locus_tag="JA154_18040"
     CDS             complement(10..708)
                     /locus_tag="JA154_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid racemase"
                     /protein_id="HAT7997725.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(870..2087)
                     /locus_tag="JA154_18045"
     CDS             complement(870..2087)
                     /locus_tag="JA154_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HAT7997726.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(2278..2691)
                     /locus_tag="JA154_18050"
     CDS             complement(2278..2691)
                     /locus_tag="JA154_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7997727.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
BASE COUNT          965 a          422 c          313 g         1072 t
ORIGIN      
        1 atgaaaaatt tatttaacta attttagata tttattgaca actgattcag ccaagcattt
       61 tgatgaatca acaatattta ttgaaggata tgatttttct aaaactacat ttaaatcagt
      121 acaagcaatt actatatttt ctatagattc atttttaaca tcttcgataa gtttattcca
      181 aatatcaata ttgtgtggat tttctttatc catttttata ttttgaataa gattgtttaa
      241 ttttatttgc cattcatctt taaatataaa ttcatgtcca ttatgtatta taccattctg
      301 gtaaattgta gactcaaaag ttgaactagt cgaaaaaaga gttacttttt gtgagatagt
      361 aggtaatttt ttaactgttt cttctacaat atttaatatt ggtatattaa tagactcttt
      421 taattcttta aagtatatat gtgctgaatt gcatggcatt gcaataaaat ttacaccagt
      481 tgattcaagc ttttgtaatc catcaattat agtttctttc ataagttcat gatttattgg
      541 acggtcaata taaaatgggg taggtaatga atatatcatc atctttggaa attcatcatc
      601 atattttgct ccatattgag attgacactc atcaataatt aaatcaacaa aaggggctgt
      661 tgaccttgga cccattccag ccaaaatacc aattaatttt tcattcataa aaattatctc
      721 ctttatatat aaatatattt atgtataaaa taaaaatcta taaaagcata aaattatatt
      781 ttataataga ttatattgta cacttatata atataaatat aaagtgattt tttagattat
      841 atattttaat atactttctt atataagaat tattttaaat caagattatc tagtattttt
      901 aaaaatattt ttacagcagt atccaagtca tctacagaaa cactttcatt aggattatga
      961 cttacgcccc cagcgcatct tataaatacc ataccaatat cagttatatt atccatttcc
     1021 tgagcatcat ggccagcacc actataaata taaaaaggat taagatttaa atctataaaa
     1081 cttttttcta tgatttttgt tattttatta gaacatggta caggaacatt ttcaaaagca
     1141 agttcacttg tataattaag ttttctattt tcacatatat gtgatatttc attaaatatc
     1201 ttttctacag aattatcaag aatttcttgt gaacaagagc gtacatcaag agtaaattca
     1261 gctctttcag gtaccacatt tgatgaagat ggatagaaat tcatctttcc tacagtagca
     1321 accaaatcag cagtcgtttt agctactttt tcgatagcta atacacattc acaggcacaa
     1381 catccagcat caagtctcat attcattgga agtgtaccag catgtccaga cttaccattt
     1441 actgatatct taaaactttt aaatcctgta atacttgata caattccagc aggaagagat
     1501 tcattttcta aaacaggacc ctgctcaata tgaaattcta aatatgcatc tacatctttt
     1561 ctaggatgca aatttgttaa gttttttgta ttaaatccaa actttgttac agcttcttca
     1621 agtgtaattc cattttcatc aacagattta agaagttcct ctttaaaagt accagcaaaa
     1681 actttacttc ccattaaaga agtttcaaat ctagttcctt cctcttcagc gaaagcaatt
     1741 agcttaatat tgaaaggata agacctatta ttgtgttttg cttcttcaag accaactaga
     1801 ggcactatta tacctaacat accatcaaat atacctccac actttacact atcttgatga
     1861 gatgcaacta ctaatgtggg agcatttgga aagcttgatt tatatgtacc tataacgttt
     1921 ccaatgtcat caatttcaat atcaagattt aaaccttcca tccatgaact aattaaatca
     1981 atagattttt tatgttcttg agtcaagata agtcttgtaa gaccatttgt tgttgaactt
     2041 atttttccta atgtttgtag ataatccata catttttttc ctaacatatt tacctcctaa
     2101 aaatataatt tttatttaag ctaagattag cttttatact tataattttt gaactttcaa
     2161 ataattttgt atgtatagta aaatactatt ttagtattaa taatgatata aaaatataaa
     2221 gtctgtagac gaagagcaga agttttaagt taaaaatata aaatatgatt tttattatta
     2281 tagtattttt ctaaaataat atctctttcc agttagagga taattttcaa gtgaaaatac
     2341 tttatggtat ccaaatttct cataaaattt aggtgcttga aaactgaatg tatctaaaaa
     2401 aacatattta caacctcgtt ctttagcaat tttttcagcc ttatataaaa gttgacttcc
     2461 agtaccttta tatctaagct tctcactaac ccataagtat tttatagaaa gccaattacc
     2521 atgagtaaca cctataaaac ctgcaacaat tttacctgct ttatctcgaa tatatattcc
     2581 taaatccact gggtttttat cttcaagtct agcaagatta tattttaata atccttgaaa
     2641 aatagtatct tcatcttgtt tttctatttt ttctgtaatt tctaattcca tatttgaact
     2701 ttctccttgt ataatctttt gtatattggt attttttaat aaaatcagta gaaagaatat
     2761 agtttaacat ta
//
LOCUS       DACULQ010000284         2768 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.286, whole genome shotgun sequence.
ACCESSION   DACULQ010000284 DACULQ010000000
VERSION     DACULQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.286"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            199..1095
                     /locus_tag="JA154_18055"
     CDS             199..1095
                     /locus_tag="JA154_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAT7997728.1"
                     /translation="MANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIYSNLKDIIYE
                     NDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAGALIYSIHPI
                     FAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDTSSTYHLANV
                     LVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTKALTGPVSRG
                     DITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIENAIENLISKS
                     EKYKEIYKLLGGKNDEKYHTDF"
     gene            1070..1897
                     /locus_tag="JA154_18060"
     CDS             1070..1897
                     /locus_tag="JA154_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAT7997729.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGTFPQEKHSFSINESELEKLY"
     gene            1916..2764
                     /locus_tag="JA154_18065"
     CDS             1916..2764
                     /locus_tag="JA154_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAT7997730.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1100 a          330 c          459 g          879 t
ORIGIN      
        1 gtcaattttt tatgaaaatg gtaagatatt tatggatggt atcttgttat aagaactata
       61 ctttaaatgt ttttaattat tttgtcttat gtaactcata tcctcaggaa tgagattaga
      121 aattaagatg ttgtatcaca aagtggaggt gatactatta aaataggctt tattggttct
      181 ggaaaagttg gaacatcttt ggcaaattat tttttatcaa aaggttattg tgtttcaggc
      241 ttttatggaa ggaatcaatt atctttatta gaatcaacaa atttaacaaa aacaaagatt
      301 tacagcaatt tgaaagatat aatttatgaa aacgatatat tatttattac aacatcagat
      361 gattctattg aaattataga caaaaaactt tccaaattta atttaaaaca taaatacatt
      421 tgccacacaa gtggctcttt aaaatctagt atattgttct gttcaaaaaa agcaggtgca
      481 ttaatttatt ctatccatcc tatatttgca ttttccaata aaaataccaa cataaaaaaa
      541 atgaaagaca tttgtttttc catagaaggt gataatgaac aagatgattt agttattcaa
      601 aatttcatag atgatttggg aaaccaattt tttataagag ataaggatac ttcatccact
      661 tatcatttag ctaatgtttt agtttcaaat ttagtattat ctttactaga tataggagta
      721 agttacttta ttaaattagg tttatctgaa gaggaatctt taaaagctat cagtccttta
      781 gtaaaaaaaa atatagaaaa tatacttgat aatggcttta ctaaagctct tactggtcct
      841 gtttctcgtg gagatataac accaataaga aaacatctat ctgtattgga taaaaaagat
      901 gaggatattt acaaaatact atccttaaac ctactaaaaa taattgcctt aggaaatgac
      961 aattcattaa aaggtataaa aaatattact atagaaaatg caatcgaaaa tttaataagt
     1021 aaatctgaaa aatacaaaga aatttacaaa cttttaggag gaaaaaacga tgaaaaatac
     1081 catacagact tttaagaatg ctaaatatga aggaaaaaaa ctatctatgc ttactgctta
     1141 tgactactca attgcaaaaa taatggatga gtgtgatata aatggtattt taataggaga
     1201 ttcattagga atggttataa aaggtgaaga aaacactcta tctgttacca tagatgaaat
     1261 tatatatcat acaaaagctg taaaaaatgg tgtaaaaaat gccttaatcg taagtgatat
     1321 gccttttcta tcttaccatg tttctataga agatgcagtt aaaaacgcgg gtagacttat
     1381 aaaagaagga ggagctcatg ctgtaaagtt agaaggtggc tcaaacgtta taaagcaaat
     1441 agaaagcatt gtaaatgctc aaatacctgt aatggggcat ttaggattaa ctcctcaatc
     1501 agtaaattca tttggtgggt ttaaggttca aggaaatacc agtgaaacag caagacaact
     1561 tattgaagat gctaaattaa tagaaaaagc aggtgcattc tctattgtcc ttgaaggagt
     1621 gcctacgaaa atagctgaga tggtaactaa ttctatttca attcctacaa taggtatagg
     1681 ggcaggaatt aactgtgatg gacaaatttt agtttatcaa gatatgttgg gaatgtttgg
     1741 agattttgtt cctaagtttg taaagcagta tgcaaatata ggagatataa tgaaagattc
     1801 aattaaaaat tacatcctag aagttaatac aggtacattt ccacaagaaa aacacagctt
     1861 ttcaataaat gaatctgagt tagaaaaatt atactaatat taaggatggt acaaaatgct
     1921 tgtaaaagaa attaaattac taagaaatat aatcaaagat tggagaaaac atggctattc
     1981 tatagggctt gttacaacaa tgggattttt acatgaagga caccaaagtc ttataaaaaa
     2041 agctgtaaaa gaaaatgata aagtagtagt gagtgtattt gtaaatccaa ctcaatttgg
     2101 accaaatgaa gattttaata gctatccaag agatatagat aaagatttta aatattgtat
     2161 ggatagtgga gcaacagtag tctttaaccc atcaccagaa gaaatgtatt taaaaggtaa
     2221 ttgtactacc attaatgttt ctggacttac agattttctt tgtggagcta aaagacctgt
     2281 tcattttgga ggtgtctgcc tagttgtttc aaagtttcta aatatagtaa cacctgataa
     2341 agcttatttt ggagagaagg atgcccaaca attagctgta attaaaagaa tggtcaagga
     2401 tttaaacata gatacagaaa taataggctg tcctataatt cgtgaaaatg atggattagc
     2461 aaaaagttcg agaaatacat atctctctga ggaagaacga aaatctgctc taatattaaa
     2521 taagagctta agtttagcaa aagaagaatt agtaaaaggc aatctaaatc cagagaatat
     2581 aaaagaatta ataacagcta aaataaattc tgaacactta gcaaaaatag actatgttga
     2641 aattgtagat agtgagacat tacaacctgt taagcaaata gaacattcta tacttgtggc
     2701 aattgctgtg tttataggaa aaaccagact aattgataat tttacatttg agttaaatat
     2761 atagtcta
//
LOCUS       DACULQ010000285         2686 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.287, whole genome shotgun sequence.
ACCESSION   DACULQ010000285 DACULQ010000000
VERSION     DACULQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.287"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            209..2020
                     /locus_tag="JA154_18070"
     CDS             209..2020
                     /locus_tag="JA154_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HAT7997731.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2108..2686)
                     /locus_tag="JA154_18075"
                     /pseudo
     CDS             complement(2108..2686)
                     /locus_tag="JA154_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890276.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1005 a          286 c          380 g         1015 t
ORIGIN      
        1 aatgatagtg ttgatgaatg tttaaaaaga aattttagaa aaacgtatat tgctgtttgt
       61 gttggaggtg atgaatttgt ataatttata ggatataaat gattaaacta tttaagataa
      121 atacatatca ctgtaaaata ttgataatca ttttttataa gagattgatt atttgcaaat
      181 tatatatatt acaaatgagg taacaataat gaagaaaaaa tatgattttt taatttattc
      241 tgctatttgg ctagttatta ttattctttt tatattttct ctatatcaca gtatagagca
      301 tattaaaatt ataagtcaca ctggtactat tcgcagtgaa actcaaaagg ttgttaagca
      361 agaattgaat aatgaaagaa atgatgactt gataaaacga ctggataata ttctgataaa
      421 gctaagaaca ggtaatggag aaaatggatt tcaaagatgt gataataaag aatttcaaca
      481 aaaactaaat caaatggatt ctatgtggga atctatgaag aaagaaatta taaaagttag
      541 aaatggtgct tcaggtgata aattatataa attgagtgaa gagtattctg tgttgtctaa
      601 tcaaattgtt tttatatctg aaaaacactc taatgcaaaa ttgtattctt ttgctactgc
      661 actatttatt tatcttatat tctcaacaat aagtctttta atttgggaat attataataa
      721 aaaaaggttt aaaagaatat tttatactga taatttaaca aaaataaaaa atcaggttgc
      781 atttgaaaac agagcgattg aaattttgca caatgcatct aacaaagagt atgtactgct
      841 aaatattgat attgacaatt ttaaatatat taatgataca catggatatg aatatggaga
      901 taaagttcta ataatagttg cagctgcact ttctaaaact tttaatatca aagaaacttg
      961 tgcaagaatt ggttcagata attttgtaat acttgcaaaa tatagagatt ctcttttaga
     1021 ggatattcgg gaaatgctaa ctaatgctat aatctctgaa ttagatatga atgtaactca
     1081 aactatatct tattgtatag gagcgtactt agttgaaatt gataatttag gttataaatc
     1141 catcaattct ataatggata aggcaaatat tgcacacaag gtaagtaaaa caagaggaat
     1201 ttcctccaca gtatggtata atgaaaatct gttaaaacag ttacaaatgg aaaatagtat
     1261 ttataattat atgtataaag cgatagaaaa tgaagaattt catatgtatt tgcaacctaa
     1321 atttcaaata tcttctttaa atgttgtaag cgcagaagct cttgtaagat ggttttctcc
     1381 agaacttggt tttttatcac cagatgaatt tattccattg tttgaaaaaa gtggatttat
     1441 tattgaattg gacttttata tgttaaaaaa agcgtgttca tttgtaagaa aaacgtttat
     1501 gaaaaaaaat caatatacat atccgattgc agttaatttt tcaagggtta ctatttacca
     1561 aaatagtttt tatcaaaggt ttttagatac tgttaaagaa tatgaaattc cttttaagta
     1621 tatagagata gaggtaactg aaagtgcttt taatgaaata tctcaaccag ttatttcaat
     1681 tttagaggaa cttaaaaagt taggattttt aatttcaatg gatgattttg gttcaggata
     1741 ctcttcatta agtcttttat gttctctatc tattaatgga ttgaagttag ataaaagctt
     1801 acttaaagaa acctttaacc gggagaaagt atatagtatt attcaatgta ttattgaaat
     1861 gtcacatcgt ataggcatgt ctgttgtatg tgaaggtatt gaaactaaaa aagacttgga
     1921 atttttaaat acagttaaat gtgatgtagg acaagggttt tatttttcta aaccgataga
     1981 agaaaaagaa ttttttaaca aatatgtaac taaaaaataa tataattatt tttatcagtc
     2041 tatacattga gtcattttat atctcgttaa atgactcaat gtactgttat gtattttaaa
     2101 atataaatta tttaaaacgt tttttatact tatatagaaa ttcttctata ttttttgatt
     2161 gttctcttat ttcaactgct tctatatcta gtaaataaag tatagaatca tttgtaagtt
     2221 caacatattc accatcttct gaaccgagtg catgtctctc aatttttaat cctttttctt
     2281 cataatattt ttttgtttct atatataatg attctcgctt ttgtatgtag tctttcactt
     2341 cattatttgg aaagttatat gaatccacta atgtttagcc atttataaaa gttttctcgg
     2401 tagcaacttt ttcatcccaa caatggattt cttcccaagt aaaaaatatc tcagttccat
     2461 ctggaatgaa tgaaaacctg gtaataatat ataaataatt attgaaattt acttcataat
     2521 attcaggaat atcttcaaat tccatatagt aatttgtata attaaaatta ctattatctt
     2581 tttttacaaa gggtattttt gcaataaatt tatatattga ttgttcattt ttaaaaattc
     2641 ctatatcata acttttttca aattgattaa tttttaataa atacaa
//
LOCUS       DACULQ010000286         2656 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.288, whole genome shotgun sequence.
ACCESSION   DACULQ010000286 DACULQ010000000
VERSION     DACULQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.288"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(21..254)
                     /locus_tag="JA154_18080"
     CDS             complement(21..254)
                     /locus_tag="JA154_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAT7997732.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            543..1133
                     /locus_tag="JA154_18085"
     CDS             543..1133
                     /locus_tag="JA154_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7997733.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEYFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            1247..2092
                     /locus_tag="JA154_18090"
     CDS             1247..2092
                     /locus_tag="JA154_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997734.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENESNKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            2089..>2656
                     /locus_tag="JA154_18095"
     CDS             2089..>2656
                     /locus_tag="JA154_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997735.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGD"
BASE COUNT         1025 a          267 c          404 g          960 t
ORIGIN      
        1 acccatattt aattaacttt ttatagtgtt ctaatagata ttattgaact taatggtaaa
       61 tataatgtgt aagtttcatt taaagttgga ttagattcat ctatatatct tgagcaaagt
      121 gtaattgata agctatctgc ttctataatt tcaccatata gaggacaact gctaaagtaa
      181 gaatgtactt caacatattt atttaagttt tcttgaacat aatcgtaaaa acggtttttt
      241 gtagtagtag ccataaaatc aactccttta ttttttatta aagttgaaac ttttaaaaag
      301 ataaatatga ttacttctat tagaaactaa gttaaaaaat aagcaaatag atatgaaaat
      361 tagaataata tattatttat atcttaatta taacactaga taataaaaaa tgctatataa
      421 aattcagaat tttcgaaagt ttttaagctt atttttaaat atactaataa tactattgac
      481 attagataga aaaagtatta tattgactat ataaattatt ttagtcaaac tggagggaaa
      541 agatgcctaa atcatattct gataaagaaa gagagtatat aataaaaaga cttaaagaag
      601 aagcaagatt gtgtatggag caatatggta ttagaaaaac tactgttgat gagcttgtaa
      661 aacgggtaaa aataccaaaa ggaacttttt atctattttt tcaatcaaaa gaattactct
      721 tttttgaggt attaagagat atacatgact ctatacaaaa agaaatctta tatgagataa
      781 atagagtaga tgaaagtatt acatgtgaac aattgacaga tatatttatg aaattctata
      841 ggatggttga tagtacttca attttaaatc tcatgataaa tggagaattt gagatattgg
      901 tgagaaagtt accagattct ataattgaag aatactttag acatgatgac tttgaaattg
      961 gagagattat atcttgcata ccaaatgcaa aaaataaaga catagaaagc tttagtggag
     1021 catttagagc agtttttctt accatgctat ataagcgcga ggttggaagt aattgttttg
     1081 aagatgcttt aagattaatg ataaacggat tagttataca acttatggaa tagaatttaa
     1141 attttgtttg ctaataactt ttattaaaaa ttattaacaa acaaaaaaat tatattttat
     1201 tgaccatata gtcaaaatag tcaaaaattt gaaagggtga tattgaatga ttaaagttga
     1261 tgatttatca tttagttata cagatagaga ttttttacaa aatattaact ttgaagtagg
     1321 aaaaggggaa atactaggat ttttaggacc atcaggtgca ggaaaatcta cattacaaaa
     1381 gattctaatt ggaatgataa ctaattatgg aggaagtgtc attgtaaatg gagtagagag
     1441 taaaagacac tcaaataaat tttatgaaaa tataggagtt gattttgaat ttcctagttt
     1501 atatgaaaag ttgactgcaa tagaaaattt aaaatatttt gggtctctat attctaaaaa
     1561 actactatct attgatgagt tattaaaatc agtaggacta gaaaatgaat caaataaaag
     1621 ggtatctgag tattcaaaag gtatgaaatc tcgattaaat tttataaaag ctttattaca
     1681 taatccagat attttatttt tagatgaacc aacaagtggt cttgacccat caaatagcaa
     1741 ggttatgaaa gatataattt tatcagaaaa atcgaaaggt aagactatta ttttaacaac
     1801 tcataacatg ttagatgcaa cagagctttg tgacagagta gcgttcattg taaatggtaa
     1861 aatatctgct ttagatacac cacataatct tattatgtca aaaggagcta ttaaagttag
     1921 atatacttac tttgataatg gagaaaaaac ttctgaatgt tttctgaaca atactgctaa
     1981 tgataaaaat ttgaatatgc ttattgagaa aaataaactt ttatcaatac atagtagtga
     2041 accaacatta aatgatattt ttattgagat aacagggagg aatttacaat gaggctaaaa
     2101 aatcttattt taggagatat aaagtttcaa tttaagtatg gtttttattt tttatatctt
     2161 tttttaagta ttatatatat ttgtattatc aatgtatttc caacttttat gagagaaaaa
     2221 atagctataa ttatgattta ttctgaccca gcagcaatgg gattgttttt tatgggagct
     2281 attgtacttc ttgagaaaag tcaaagggtt ttaaattcct tagctgtatc tccagtaaaa
     2341 gtatctgagt atattttatc aaaggtaatt tcattaggag taatatctag tattgtagct
     2401 atgtttattg ctattacatt aaatttagat aatattataa tatcaactat aggtaccttt
     2461 ttttcttcta ttatattttc tttgcttgga ttgataattg caagtaaagc atctagtttg
     2521 aatcagttta ttgttttaag tattcctatt gagattatat gctttattcc accaatactg
     2581 aatgtacttc ttgacacaaa atcatatgca aatttgtacc cttttaatat atgtatttct
     2641 cttattagtg gagata
//
LOCUS       DACULQ010000287         2626 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.289, whole genome shotgun sequence.
ACCESSION   DACULQ010000287 DACULQ010000000
VERSION     DACULQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.289"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            90..209
                     /locus_tag="JA154_18100"
     CDS             90..209
                     /locus_tag="JA154_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAT7997736.1"
                     /translation="MIRTNIILIGKFKKIKQLANRGYIYKTQSDKIYLENNVK"
     gene            227..928
                     /locus_tag="JA154_18105"
     CDS             227..928
                     /locus_tag="JA154_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997737.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            1010..1618
                     /locus_tag="JA154_18110"
     CDS             1010..1618
                     /locus_tag="JA154_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HAT7997738.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            1942..2406
                     /locus_tag="JA154_18115"
     CDS             1942..2406
                     /locus_tag="JA154_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HAT7997739.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
BASE COUNT         1095 a          214 c          460 g          857 t
ORIGIN      
        1 agtcttgcct ccataaaaat atattataca ttataaaaca agatatttgt tttggaaagt
       61 taaatttgta attagtacat ataaaatatt tgataagaac aaatataata ttaattggaa
      121 aatttaaaaa aattaaacaa ttagcaaata gggggtatat ttataagaca caaagtgata
      181 aaatctattt agaaaataat gtaaaataac aggaggataa agcaaaatga aatttatagt
      241 agaaaaagaa gtttttgata aattagaaaa tgtatgcttt ggtgtagtag ttgcaaaagg
      301 aatagataat actaaagaaa tagaaagaat aagcaacctt ttagatataa gtatagatag
      361 agtagaagat tacttcaaag ataaaaaagt aaaggaatca gaagaaatta taccatatag
      421 agaggcattt agaagtctag gcatgaaccc aaataaattt atgagttcga tagaagcaat
      481 gacaacaagg gttgctaaga ataagaaact tcctcatata aatcctattg tagatttagg
      541 aaactctatt tctttaaaat atttacttcc aatgggtgct catgatatgg attttagaaa
      601 tgatgatgta tatgttagat tttctaaaaa aggggataaa tttgtaccat ttggagaaac
      661 tgatgttgaa ttaatggaag aaggagaatt aatttattca gttggagaca tggtaaaaac
      721 tagaagatgg atatggagac aaggagaaga aggaaaaatt actaatagct caaaaaatat
      781 attttttcct atagatggtt ttactgatgc taatttagat aaggtaatga gtgcaagaga
      841 agaattagca aaattgttaa aggaaatatt taattgtgaa attaaagtag gttttgtaga
      901 taaagataat cctgaaatgg aaatataata agttgttgaa tacttgttaa gatattatat
      961 ttacttaatg atgttaaaaa ggtattaaaa aagaaaggtc gtagtaaatt tgaaaaaaac
     1021 tataataaag aatgtaaatc caatgattga aaataagttt ataggacttt ttgaaataga
     1081 gtataaaaac aaattagatg aagataaggt ttggatggta gcatcaagaa aaagtagtga
     1141 gcaattaaaa aatatttatt tgaagaatga agaagatagt gtagatgcag ttgtaatagt
     1201 tgggcttcat aaaagtagta aaaaattaat tttaataaga caatttagag tacctattaa
     1261 tggatatata tacgagttac cagcaggtct tgttgatgaa ggagagtcta tagatatttc
     1321 cgttgaaaga gaacttagag aagaaacagg acttactctt ttagaaatta aaaaaaataa
     1381 aagtattgat aaagtttatt tatctcctgg aatgagtgat gaatctgttg cctttgttta
     1441 ttgtatatgt gatggtgata taacagatga atttttagaa ccagatgaag agattgaagc
     1501 gcttttaatt tcgcaagaag atgcaaaaga gattttacaa agtaatcata agatagacac
     1561 aaaagcattt ttgattttac aaatgtttgt gagtttagga gctaaattat ttgaatagat
     1621 atatagaaaa gaggttatat agaagagtaa aataataaag tttattttac ttgtgtaaat
     1681 tataatctct ttttttatac aataaagtat aattttgtgt atttttaaat aagtgatttg
     1741 actcttaaat tttcataata tgttataaac caatttaaga tactacaata attaaaattc
     1801 caaaattatt taacatattt gcaattaaaa tgagaaaatg taaaggctga atgattgaaa
     1861 aatttgatgt aaaatagtag cttagtttaa tactatagtt tgtagggtta aaaattagaa
     1921 attgtaaggg agataaattc tatgaaaaaa ttagttatta ttttaattag tatcattagt
     1981 atggttagct tgacagcttg taaaaaaagc aatgaaagta aatataataa tttaaacaac
     2041 aaagaatccg gacaaacaac cattgaaatg gacttagata aaaattatga tacttcagac
     2101 ccatttgtta atgcacgttt gttttgtgtg tctaatgata tagatgtttt agacacagag
     2161 atttctttta aaatggatgg agatagtggt attgtggaaa taaaggacaa taaaacagat
     2221 gaaactttat ggagtaatac atggcatgga agcgttgata atgatacatt cacaatttct
     2281 ctttctaata tacaaaaaga gaaagagtat gctatatgct ttactggaat taaaataaat
     2341 catgcagttg taaaggttac ttttgaaagc aatttagtta aggagaaaga aaaaccctca
     2401 aaataaattc cagcttttct ataacttgat attcagtaca taaaagcagt tgatatttat
     2461 gattgactgc tttttcttat caaaaaggat aggttgtgca tttttacttg aaatagttta
     2521 tgcacatcaa aagttggtga gcatgatatt ggaaatggta agcatgaaat cttgataata
     2581 caaggaaata aatttaagtt ttcaattaag tatatttcca taattt
//
LOCUS       DACULQ010000288         2578 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.290, whole genome shotgun sequence.
ACCESSION   DACULQ010000288 DACULQ010000000
VERSION     DACULQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.290"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            66..971
                     /locus_tag="JA154_18120"
     CDS             66..971
                     /locus_tag="JA154_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997740.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            980..2164
                     /locus_tag="JA154_18125"
     CDS             980..2164
                     /locus_tag="JA154_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7997741.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            2285..>2578
                     /locus_tag="JA154_18130"
     CDS             2285..>2578
                     /locus_tag="JA154_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HAT7997742.1"
                     /translation="MIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQGV
                     VKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDY"
BASE COUNT         1065 a          212 c          442 g          859 t
ORIGIN      
        1 ggagtttttt atatatacaa aataaaattt ttaaaattaa gcaataaaaa aggtaggagg
       61 attttatgtt tttcaaagat attgaactaa attcaaaaaa agagttagac ccatattttg
      121 atttagtaga ttatgaagct tgtgagtact gttttagtac tttatatatg tggcaacatg
      181 tttataagac aggatattat ataggtgaag attttgccgt tttagttggg gaatacgaag
      241 gagatagctt ctcaatatta ccacttgcta aaaaagataa acttccagaa gttgtagatt
      301 ttgtccttga atatttttct aaaaataata aaaaaatata cttaagaggt attactactg
      361 aagtagtaga gtttttaaaa gaaaaatatc caggtagatt tgagtatata gaagaaagag
      421 atttatttga ctatatttat gatgcagaaa gtttaagaac tctagctggt aaaaagaatc
      481 aaaagaagag aaaccatata aactatttct taaaagagta tgcaggtaga tatgaagcta
      541 aattattaga taaagaaaac tttgatgagt gtttagtact aatgaaggaa tgggaaagta
      601 ataaggaaga aaataatgaa tttgatgaaa gtatggatga tgaattaata ggtatcaaaa
      661 agatatttaa ccattatgac atattaaaag ataaggtaaa agtgtttggt gtttatgtag
      721 atggaaaatt agaagctttt agtataggag agcttttaaa tccaaatatg gcattgattc
      781 atatagaaaa agctaatccc gatataagag gtctgtatcc atttataaat caacaatttt
      841 tggtaagtga atttaaggac gttgagtttg taaatagaga agaagattta ggtattgaag
      901 gattaagaaa agctaaactt tcttatcatc cttgcagatt tgttgaaaag tacagtgtta
      961 gggaggctta atagactata tggagattag gtatgctaaa gaagaagaga tagaaagtat
     1021 aaaagatatt tggagttatt gttttaacga tacggagagt tttatgaagt actattttaa
     1081 tgataaatat aaatctgaaa atactgtagt agctttagat gaaggaaaaa ttatttcttc
     1141 tcttcagcta aatcagtata agttattact aaatagcaag gtatataata catcatatgt
     1201 ggtaggagtt tctacacttc cagagggaag aggagctgga tatatgaaca aagtaatgaa
     1261 atttacttta aacgaattgt ataaaaaagg gcaattagtt tctatattga tgcctattga
     1321 ctatagactt tatagaagat ttggatatga gcattgttac gaccagattg aatatactat
     1381 taacacagat gatttaaaaa attttaagtc aagtggaaaa atgataaagt ctaatttatc
     1441 acaaattgat gatttgatac aaatagatag aactttttta aatgaggtta atggtaatgt
     1501 gttaaaagat gaacattatt atgaaaactt atttaaagag attcaaagtg aagatggatt
     1561 tttatacatt catgaaggaa atgaaaaaga tggatatata gtttattttc ttcaggaaga
     1621 taaaatgttt gtcagagagt tattttataa aaatatagat gcccttaaat caatgcttaa
     1681 atttatatat aatcacaata ctcaatgtaa gatagttact atatctactc ctacaataga
     1741 taagataagg tttatattag acaaccctaa agatagtgat ataaaaataa aaccttttat
     1801 gatgggaaga gttataaatg taaagaagtt tattgaagat atagatattg agaaggatat
     1861 taatagctca tttaatttat taatagaaga caagtttata gatgaaaata atggtctatt
     1921 taaaatttct atacagaata agaaggtatc tgtagagcag ttagataaaa aaggtgcaga
     1981 aaagcctcaa gaagattttg atattaaatt agatataaat accttaacac agttatcatt
     2041 ttcttatata gatgtaaatg aagctatttt cttaaatgat ataaaagatg tttcagaaga
     2101 aacactagaa acacttaatt gtattttttc taaaaaaaat aactatataa atgaatatat
     2161 ataggtagag tacgttaaaa gattctaaaa atcttttgac gtattttttt gtttagataa
     2221 caatttatct tgtttaaaaa ttttctattt gaaaataatc taaataatat acttttatat
     2281 gggagtgatt ataattcaaa taagaagacc attattaatt atattaatgt ttgtggtagc
     2341 tatatcattt atcattacaa ataacacaaa aaacgacgaa gaacttaatg ataagataat
     2401 aactatacaa ggagttgtca aaggaaaaat tgaaaagaaa agatacaatc agtacaaggt
     2461 aggggttttt ctaataaatg attatacaaa aaataaaaat ttgaagattg gtcagaaagt
     2521 taatattaca ggtaaattca aaagtttaga taaaatgaag tatgatgatt ttgattat
//
LOCUS       DACULQ010000289         2535 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.291, whole genome shotgun sequence.
ACCESSION   DACULQ010000289 DACULQ010000000
VERSION     DACULQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.291"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..292)
                     /locus_tag="JA154_18135"
     CDS             complement(<1..292)
                     /locus_tag="JA154_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997743.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKS"
     gene            complement(510..1466)
                     /locus_tag="JA154_18140"
     CDS             complement(510..1466)
                     /locus_tag="JA154_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HAT7997744.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            1909..2277
                     /locus_tag="JA154_18145"
     CDS             1909..2277
                     /locus_tag="JA154_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT7997745.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          920 a          331 c          326 g          958 t
ORIGIN      
        1 aacttttatt tatttcataa tttaattcaa ataaatcttt taagatataa tttataagta
       61 ttctttccca acccattttt aaattaactt tagagtctat taatatattt tctatctttt
      121 taaatatttc tatatttgct ggtattatac tgagctcaat attactttta aacgggttag
      181 actgtaaatc tgttataagt atatttttta attcctctgt aagttcagaa attaaactat
      241 cacctatgta tttttttatg atttcctctc taatttcttt taaatttagc aatattagta
      301 cctgctactt acatagtagc ttttcctttc cttttaatac tattattcat ttttgaatct
      361 tatttagata tattttaaga ttaaaagtct ttagctatat aattttttac ccatttttaa
      421 cttatattaa catattacag gcttatatct ccaccctgtt tacattgagg atggagtata
      481 ttgcctattt tgataaaaat gataggttgc tatttgccta ttcctttttc tgataatttt
      541 tctaacatat ctttggtcat tttatctaaa tcataactaa acttaaagtt ccattctttt
      601 actgcactac tactgtctag agagttaggc caagaatcag ctatactttg tcttacaggg
      661 tctacatcat attctatcac aaaatcagga atatattttt ttatagaatc agctatttct
      721 tcaggagaaa agctcatagc agtaatatta aaagcatttc tatggatgag tttatcagct
      781 ggagcttcga ttaaatccac tattgattga agtgcatcag gcatatacat catatccatt
      841 tttgtacctt ctgctatgta agatttatat ctcttatttt ttaatgcctc ataatatata
      901 tcaactgcat aatctgtagt tcctccacca ggaggcgtaa cataagaaat aagacctgga
      961 aatctaacac ctctagtgtc tactccaaat tttgagtgat aataatcaca tagtaattct
     1021 ccagaaactt tagtcacacc atacatagta ttaggtcttt gtaatgtatc ttgaggagtc
     1081 atattttttg gagaattttc accaaaagca gcaattgaac taggtgtaaa aaatttacaa
     1141 tccaattctt tggctatttc aagaccattt attaatccat tcatatttaa gttccaagct
     1201 tctagaggtt tactttcagc aactgcagaa agtaaagatg ctagatgaat tatagtatct
     1261 acatcatatt tttttgcaat ttcaaaaaaa cgattcttat ctgtaacatc taatatctca
     1321 aatataccag attcacatac tggattacct tcttttactc tccttgaact agcgattaca
     1381 ttttgttctc catactcatt tctaagcttt attgttaatt ctgaacctat ttgtcctaat
     1441 gcacctgtta taagtatttt tttcaatttt acaacctcct aagggtttaa tttatgtagt
     1501 actacttatt tttattttag caaatctttt gtttaaatac aaagaattaa agataataat
     1561 atacgactta attgaatgaa gtgcagtaag tgcgtaaaaa tttaaagcta atatataatg
     1621 gtctattgta taacaataaa agagtagatt aaattattat atatagattg tgtgactaag
     1681 tcacagaaaa tagagctaat ttaatatata ataaaagcat gtcaagtaaa gctttaaatt
     1741 aaagtaactt tatataaaaa gtaatataaa ttgataaaat tataaattta tttatagtga
     1801 atataggttg gattttatag atacaaaaat agtatatatg atatgtagac cattagcatg
     1861 tttaaagtag caaaacatat aaaataaaat ttgaaaggaa gtataaaaat gacaagacca
     1921 tcaatatatc atagccaagg atacacttgt gcagaggcat taataaagtc ttataatgag
     1981 gaacacaaca cagatatacc aatctcaata ggaagtggta tgggggttgg aatgaatgtt
     2041 ggaagtgttt gtggagctgt aaatgctgct gttgttataa tagggtattt aaatgggcga
     2101 aatagtaatt tagatgaaaa tatagcaagg aagtattcaa gagagctatt gaatagagta
     2161 agaaataaat acagctcaga aatatgtgta tctcttaaaa aaaataaagt aacttgtgaa
     2221 gaaataataa atttcacata tgatgctcta aatgatatgc taaaaaatca aaaataattc
     2281 taagaaacaa gtattttaca ttcgatagaa tacttgtttt tttatgatat ttttaataag
     2341 tattagttta ttataatttt taaatcaatt ttattatatt ttaattgaaa taatctactt
     2401 tgaaaattac taactgacaa aagaatatga ttatagtata gaaaaaatgt aaattattta
     2461 atattaagtg agagtatatg cttacctata taaaatcgta taaattaatg gctaaattta
     2521 taaattataa agaaa
//
LOCUS       DACULQ010000290         2533 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.292, whole genome shotgun sequence.
ACCESSION   DACULQ010000290 DACULQ010000000
VERSION     DACULQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.292"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            176..401
                     /locus_tag="JA154_18150"
                     /pseudo
     CDS             176..401
                     /locus_tag="JA154_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583656.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1644..1900
                     /locus_tag="JA154_18155"
                     /pseudo
     CDS             1644..1900
                     /locus_tag="JA154_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2010..2432
                     /locus_tag="JA154_18160"
     CDS             2010..2432
                     /locus_tag="JA154_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997746.1"
                     /translation="MEYSCLKTLAGKHRSKVSKIRAMYKDGTGKWAIPYETKTVIKKM
                     YFANYADCKGKKFTDIVPQTAKNYSHDVTTLESRLKAKICEVCGCTESDRYEIHHVNK
                     VKNLKGKSEWEKIMIAKRRKTIVVCHKCHMAIHHGEKK"
BASE COUNT          971 a          433 c          528 g          601 t
ORIGIN      
        1 ttagaagtag attaaagaaa gggggacaga aaaaaaaggc actaccccgt aagataacac
       61 gggaaaccac ctgcctaacc gaaaggtgaa aactgtacgg gaaaatagca cggcaggaaa
      121 gcagaaagtt gttcaaagac aatagagcac gactgaactg caaggttaag tgaatatgag
      181 gataaaacta gatttgttga atgtgagttc caaacctctg ttacgtgtgg acacaggaaa
      241 cttgtctgaa acctgtggta tgagtaacgc tgaaaataaa tgcctttcag tgggttatca
      301 attaactcat acggtacgct ggagaaccag tagtaacgga acgaaagcat atccgacaat
      361 tcacaccagc ctactacttt cgtcaactgg agattaccta aaccagaatg cctacaatgg
      421 ctatgtgtaa tgctcttgta gtgataaatc acaaggctat gctaagtgag atgacgctga
      481 aaatctggca aggtaacgga gctttcatag tagtctgaga accctgaaaa gacagggaaa
      541 gccgtaaaat cggatacggg aaaaccgttc acatggcgaa ggaaagcagt cttagcgaat
      601 taaaattaga aattgattag ggaggaaaac ctcaaatgaa accaacaatg gagattttaa
      661 ccaaattaca ggaaaattca aaaaagaacc atgatgaagt atttacaagg ctctatcgat
      721 atttactccg tccagatata tactatgttg catatcaaca cctctactcc aacaaaggag
      781 caggaacaaa gggtgtaaca gacgacacag cggacggttt tagcgaaata tatattgaaa
      841 atatcattga agccttaaaa aatgaaatgt atcaaccaaa accagtaaga cgtacataca
      901 tcaaaaaatc aaatggcaaa atgcgtccct taggactgcc tgtctttact gataagctga
      961 tacaggaagc aatacgcatg atattagaag caatctatga gcctattttt tcagactact
     1021 ctcacggttt ccgaccagca agaagttgtc atactgctct tgctcaaata aaaaaagaat
     1081 ttacaggagc acgctggttt atagagggcg acataaaagg gtgctttgat aacatcaatc
     1141 atgcggtact tgtggaaatt atcaatcaga aaattaaaga tgcacgtttt ctgaaactca
     1201 ttcgttcatt cttaaaggca ggttatatgg aagattggaa ataccacaaa acatatagcg
     1261 gttgccctca aggtggcatc atttcgccta ttcttgcgaa tatctacctt aatgaattag
     1321 accgtcacgt aatgaagata aagaaagagt ttgatgtggc aacaaaggca cgttatacac
     1381 cagaatatac aaaactggta ggactacgcc aaagattaca caacaaaatt aaaaatagca
     1441 acggtataga aagagaaaag ctgattgaag aatataaatc tgcaacggct caaatgctta
     1501 aactaccagc gaagcaatgt gacgacaaga aaataaaata tgtgcgttac gctgatgatt
     1561 tcctaatagc tgtgaatggc aatcgacagg attgtgagaa gattaagcaa gaacttacag
     1621 aatttataag taccacatta aaaatggaac ttagtcaaga aaaaactctg attacacaca
     1681 gtaatactcc tgctcgtttt ctcggatatg atgtcagagt acgccgtgac cagcagatta
     1741 aaccaaaagg aaaattcaaa acccgttcta tgaataacaa ggtggaattg agtattccat
     1801 tcaaagacaa gatagagaaa tttttatttt caaatggaat tgtaaagcag aggtcagaca
     1861 atgggaaatt agaacctata caccgaccac aactgcttaa taggacagat ttggaaatag
     1921 tgactattta taatgcagaa ttaagaggta tctgtaatta ttatgggttg gcaagcaatt
     1981 ttaacaagct aatctatttc aattatctta tggaatatag ctgtttgaaa acacttgcag
     2041 gaaagcaccg ttccaaagtt tctaaaatta gagctatgta taaagacggt acaggcaaat
     2101 gggctattcc ttatgaaact aaaacagtaa taaagaaaat gtattttgcg aattatgctg
     2161 actgcaaagg aaagaagttt actgatattg ttccacaaac agctaaaaat tattcccatg
     2221 acgtaacaac attagaaagc cgattaaagg cgaagatatg tgaagtatgt gggtgtacgg
     2281 aaagtgacag atacgaaatt catcatgtaa acaaggttaa aaaccttaaa ggaaaatcag
     2341 aatgggaaaa gataatgata gccaaaagac ggaaaacaat cgtggtatgc cacaagtgtc
     2401 acatggctat acatcatggc gaaaagaaat aacctgtaac ggagcaaaga tggagagccg
     2461 tgtgcattga gaaatgcaag cacggttcgg ggagaggact gcacaaacct accctagaaa
     2521 taaggaaagg cgg
//
LOCUS       DACULQ010000291         2502 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.293, whole genome shotgun sequence.
ACCESSION   DACULQ010000291 DACULQ010000000
VERSION     DACULQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.293"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            73..618
                     /locus_tag="JA154_18165"
     CDS             73..618
                     /locus_tag="JA154_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIC"
                     /protein_id="HAT7997747.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            631..1644
                     /locus_tag="JA154_18170"
     CDS             631..1644
                     /locus_tag="JA154_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HAT7997748.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1866..>2502
                     /locus_tag="JA154_18175"
     CDS             1866..>2502
                     /locus_tag="JA154_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAT7997749.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFY"
BASE COUNT          938 a          262 c          491 g          811 t
ORIGIN      
        1 atgtaatttg taaaatttat ggtaagcagt aaaattaaaa atttaaagat attataggga
       61 ggttataatt ttatggaaaa cgttattaca ggtctaagta aaggtgcaga gtggtttatc
      121 ggtttgtttc agaaaggggg agaacaattt ataggtcttg tttcaggtac tcttccaacg
      181 ctaattgtat taatggtagc tattaactct ttgattaaga taattggtga ggaaagagtt
      241 aataattggg cttctaagct aggaaagaac tcttttacaa gatatattct acttccatta
      301 gtatcagtat ttttcttggg aaatcctatg tgctatacat ttggtagatt tttaaaagaa
      361 gagcacaaag caggatttta tgatgcagca gtttcatttg tacatccaat tacaggtctt
      421 tttccacatg caaatgcagg agaacttttt gtatggttgg gaatatctgc aggtttgact
      481 actttaggta aagaaactac tacattggca ttgtggtatt ttatagttgg tctaattgta
      541 atttttataa gaggtatagt aacagaaaaa atgtatgctt ttctaacaag aaaaaataga
      601 gcaacaaata aggcttagga ggtagataaa atggaggaga ataaaatatt aaaaatagaa
      661 aagggaactt caggctgggg aggtcctttg tatataaaaa aagaagggaa tagaaataag
      721 atactgtcaa tgactgctgg tggtatccat gaagtaacat taaaaataaa agaactactg
      781 ggttgtgaaa ttgtagatgg atttaaaact ggagtttctg atgaagaagt agcagtagtt
      841 attattgatt gtggtggaac tgctagatgt ggagtttatc caaagaaaaa aatacctaca
      901 ataaatgtaa atccagttgg aaaaacaggt cctttagcaa aatttataac agaagaatat
      961 tatgtatcag atgttaatcc taactgtata agtgttgtag atggggaaga tatgccacaa
     1021 aaatctcaag aaaataaaag cgaaaataag agttctatta gaaagcctga taattatgat
     1081 gaggttaagt caaaagcaca aggtgaatat gctaaaaaga acataatact ttctattggt
     1141 caaggggcag ggcaagtagt atcaaaattt tatgatgcag gtcgagatac tatccaaatg
     1201 gtaatgaata acgtaatacc atttatggca tttgtaagta tgcttatggg aattatttta
     1261 gcatctggac tgggagactg gattgcaaga gttatatctc cacttgcagg taatattggt
     1321 ggattattga ttatatcagt aatttgcaca cttccattct tatcaccaat actaggacct
     1381 ggtgcagtta tagctcaagt agtagggact cttgtaggta cacaaattgg tcttggtgca
     1441 ataccagcat atttagcact tccagcttta tttgcaataa atgggcaagc tggatgtgat
     1501 tttgtaccag taggattaag tttgggagaa gcagaaccag aaactgtaga gtatggtgtt
     1561 ccagctttat tttactctcg tttaataact ggtccaatag ctgttattat tgcatatgga
     1621 gttgcagtat ttgcattgag ataatattta atgtcaatat aaggcttgga attaattaat
     1681 aaaattgtaa atataaattt aagctagtca agattttttg ttaatgaaag actagctttt
     1741 atactattaa ttagctgtaa ttatcgagtt agattaatac taaattttcc tttattaaag
     1801 tgaaatttta tgtattaatt tggtttcaaa tttgtactag aatgaaatca aggaggagat
     1861 acaaaatgag tgtaactgag aaaattcctt ctattgaccg caagatattt aagatattga
     1921 ttatgtgtag taaaaaagag tttgtgagta ttaactcaat agcaaatgaa ttgaatgtca
     1981 caactagaag tgtaaggacg tatataaaac aattgaataa agatttaggt aatgatatag
     2041 cagtaatcaa atatataaag ggtcaaggct ataaactaga aataaaagat gagcagatac
     2101 tcaataagat tattgatatg aatagaaaaa atgtattttc attaaattca aaagaagata
     2161 gagtagagtt tatactaaat tacttgatag aactagatgg atttattaca cttgatagtt
     2221 tagctgatga gatgtgtgta ggaagaacta ctttagtaaa tgatttccag tatgtagaaa
     2281 aagttttggc ttcatataat ttaaacttga ttaaaaaaca gaatacaggt atgaaattaa
     2341 atggaaatga acttgatatt agactgttta tattaaatca gttgtataaa aactcaagga
     2401 aagatttcag taattcaaag tattttaaag gaatcaaaaa agaagaaatc ataaatttag
     2461 aagaaaaact attgacttta tttaaaaaaa ataattttta tg
//
LOCUS       DACULQ010000292         2470 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.294, whole genome shotgun sequence.
ACCESSION   DACULQ010000292 DACULQ010000000
VERSION     DACULQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.294"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            7..438
                     /locus_tag="JA154_18180"
     CDS             7..438
                     /locus_tag="JA154_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7997750.1"
                     /translation="MKNALDLIWNVFEEFESPDYCDEGITTFHQFIEYNSIIEKIKEK
                     TLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLMTTGIQYCICNKFNL
                     TEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(807..1364)
                     /locus_tag="JA154_18185"
     CDS             complement(807..1364)
                     /locus_tag="JA154_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7997751.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSFL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            1622..2320
                     /locus_tag="JA154_18190"
     CDS             1622..2320
                     /locus_tag="JA154_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7997752.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNIPTKILYGKHDELTEIGVVEKFVEKFNCNLTIVENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          918 a          321 c          295 g          936 t
ORIGIN      
        1 gatgatatta aaaatgcatt agacttaatc tggaacgtat ttgaagaatt tgaatctcca
       61 gattattgtg atgaaggaat tacgacattt catcaattta tagaatataa ctctattatt
      121 gaaaaaatca aagaaaaaac tctcttcttt tggggttgtt ttgactctaa aaaacttatt
      181 ggagtcttag ctattagaaa tttaaatcat atatgtatgt tatttgtaga taaaaactat
      241 cataaacatg gtgttgcaaa aaaattaatg actacaggca tacaatactg tatttgtaat
      301 aaatttaatt taactgaaat aactgtaaat tcatcacctt atgctgttga agtttatcat
      361 aaattaggat ttaaagatac tgctgttgag caaattgttg acggtatacg ttttacacct
      421 atgaaattgt taatataata aattaaataa aaagcaaagt ctcttaaata ttttatagtt
      481 actttgcttt taaaccaaga tttaaaataa tactatcttt tttgtttttt aataatatac
      541 tttaaaatcc ttactaatat gaactataat ttattttttg atatcctttt tgattaacat
      601 ataaattaat tcaattatta aacataaact atgttgtttc tctttatatc tcttctttgt
      661 aaacaatatt gggcatttga aaataaatat tcttaaaata tactgttctc ctgttgattt
      721 tcctttggta caaatgataa aaatttaatt tacatgcccc ataatgaagt gttcaataaa
      781 taagttaaaa taatttattt acctccttaa tcatattttt tatgaatacc taataacaca
      841 gtatcaatag aatcgactaa accttttata gcttcttctt tactcaaaaa tgatttccca
      901 agttgcaaag tcgaataacc atgaagtaca ctcattatta agtttaatac ttcacctgta
      961 ttctttgtat ttagattaca agaaagaata agtttttcta ataaggattt atattcatca
     1021 aagatttcta atgtttcatt atttccatgc caagtagccc actgaattgt ttcataaaca
     1081 ccaggattag aaattataaa attaaagtat gctatactta tacactttat agctgtatct
     1141 ccaaaatttc caatagccgc ctttatcatt tgactattca tctctctcat tcctttatga
     1201 gctacttcaa gtaataaatc atctagactt tctatatgat tgtacaaaga tggtgtacgt
     1261 attcctagct tttctgcaat aacttttaaa gaaactttgt taagcccatc tttattaact
     1321 atatctgatg tagcttgtat aactatttct ctactaacac gcatgatttt ccctctttca
     1381 tttaaaatta attactttat tagtttaact aatctaatta gtttcgtcaa ttattgtaat
     1441 ttataaaatt ctttatacta ttataaaatc ttgacagttt aattataatt taatataata
     1501 ctaattaaat tagttttaat actaatataa ttagtattat attaaattct atattacaac
     1561 attattttat agtttgatta atttaataat cactaatata tcaaactgaa aggagatttt
     1621 tatggaaaaa aaatatttcg aaattcatgg agttccttca atcctttggg gaaatccttc
     1681 aagtaaatta tatatttaca tacatggaca atgtggcaat aaagaggagg ctgaagcttt
     1741 tgctaatatt gcagttccac atgagtgtca agttttaagt gttgatttgc ctgaacatgg
     1801 agaacgtaaa agagaaaaaa attcatttaa cccatggcat gttgttccag aattagtatc
     1861 tattatggaa tatgcaaagt gccactggaa tcaaatttcg ttatatgcaa atagcattgg
     1921 agcatggttt agtatgttaa gctttgaaaa agagaactta agagagtgta tttttgtatc
     1981 tccaattcta gatatgcaac agctcatttc aaatatgatg ctctgggcaa atgtatcaga
     2041 agaacagctt aaacatgaac taattatccc aacttcattt gggcatactc tttcatggga
     2101 atatctacaa tatgtaaaac aacatcctat cactcaatgg aatataccaa caaaaatatt
     2161 atatggtaaa catgatgaac ttactgaaat cggtgttgta gagaaatttg tagaaaagtt
     2221 taattgcaac cttactattg tagaaaatgg caaacattgg tttaacacac cacaagaatt
     2281 aaattcatta tatgaatggt taaatacttt agttaaataa aaaataatta tattattaat
     2341 atgattaaat tatttataaa tgttatttaa ttaattttat tttagcatta ttaaaataac
     2401 tacataaaga ataaattagt tattattaaa gaaattaaaa gtatatagag tattaataat
     2461 tttttaataa
//
LOCUS       DACULQ010000293         2453 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.295, whole genome shotgun sequence.
ACCESSION   DACULQ010000293 DACULQ010000000
VERSION     DACULQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.295"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1810
                     /locus_tag="JA154_18195"
     CDS             <1..1810
                     /locus_tag="JA154_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HAT7997753.1"
                     /translation="DMNNYYKILTFIKEDEKVAEKLKKFDNDISIIDKLVELNDKIYL
                     LSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYENIILNIPSPILVRSAEGGIKK
                     NKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFFDNREFKRLNLTQEKKAEIVS
                     YNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFKLLTFRDITKEINILRTVNEQ
                     KIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGISSDVLGVTTQKYRNILVKKY
                     INNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKRSRVRIIKDLSVQRESERLKK
                     ALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIENLYKKGKIIDFQDILKEHIK
                     ITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIIDITERIVTSISSYAKSKGIDL
                     IFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGEILVGIYKKDETVEIIIKDTG
                     VGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKSLIEIQNGKIYIDSKVGEGTN
                     VKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            1919..>2453
                     /locus_tag="JA154_18200"
     CDS             1919..>2453
                     /locus_tag="JA154_18200"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087994.1"
                     /note="responsible for recognizing base lesions in the
                     genome and initiating base excision DNA repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAT7997754.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDI"
BASE COUNT         1085 a          193 c          413 g          762 t
ORIGIN      
        1 ggatatgaat aattattata aaatattaac ttttataaag gaagatgaaa aagttgctga
       61 aaaattgaag aaatttgata acgatataag tataattgat aaactggtag agttaaatga
      121 taaaatttat ttgttaagtt taagtaataa taaagatgaa agcaattact taataagttt
      181 aaatgatatt acaaatgaaa tcaaaataga tgaagaaata agaaatataa ataaagacta
      241 tgaaaacatt atactaaata ttccatctcc tatacttgta agaagtgctg aaggtggcat
      301 aaagaagaat aaggtaataa gtataaataa aaaatatgaa aagagtttta aatgtgtttc
      361 aagtgattta gtcgatatga cattagaaca atattttgaa acttttaaca tagatttctt
      421 tgacaatagg gaatttaaaa gattaaatct aacacaagaa aagaaagctg aaatagtaag
      481 ttacaatgac acagcaaact gtatagttaa ttttgtgatg tctgactcag aaggtgaaga
      541 gcatgttgaa gaagttagag ttggagacta ttggtcagat aataaactct ttaaattgct
      601 tacatttaga gatataacta aagagataaa tattcttaga acagtaaatg aacaaaaaat
      661 aatatatgaa aaattactgg atgttatacc agaggctatt tttcttgagg atttaagtac
      721 atcaagagta ctatatacaa atagagcctt tagagagttg ttcggaattt ctagtgatgt
      781 attaggagtt acaacacaaa aatatagaaa tatattagta aaaaaataca taaacaattt
      841 aaatatagga gaaagagaga agagtatcca tatagtaaat gaaaataatc acattaaaga
      901 agtaaaaatg atatcaagaa cattatactt tggacagaaa agaagtagag tgagaattat
      961 taaagattta agtgtgcaaa gagagtctga aagacttaaa aaagcattaa taaaacaaag
     1021 acagtatgac caaatgaaga tggagtttta tgctaatata tctcatgaac ttaagacacc
     1081 tttaaataat atatatagtt cagttcaact tattgaaaac ctttataaaa aaggaaaaat
     1141 aattgatttt caggatatac taaaagaaca tataaaaata actaaacaaa atatgtttag
     1201 attattgaga ttaatagata atataataaa tatttctcaa gttaagtcag acatatataa
     1261 gattaaagca gttaattttg atattattga cataacagaa agaatagtta catctattag
     1321 tagttatgct aagtctaaag gaatagattt aatttttgat acagatgaag aaactgttat
     1381 ggtagggctt gacccagaat cgatagaaag aataatacta aatatattat caaatgctat
     1441 aaaatttact ttagaaggag gagaaatcct tgtaggtata tataaaaaag acgaaacagt
     1501 agagataatt ataaaagata ctggagtggg catagacaaa gaaaaattaa atgatatatt
     1561 taatcgattt aaacaaattg aaaacagtgg tatatcaaat gaatttggaa gtggtatagg
     1621 tttatgcctt acaaaatcat tgattgaaat tcaaaatggg aagatttaca tagatagtaa
     1681 ggtaggagaa ggaactaatg taaaagttat attcccaatt aaagaggttg ttgaagaagt
     1741 atatgataat tcaaattaca atgataatat tgaaaagttt gagatagaat tttttgatat
     1801 atataaataa taaatgtcta aatttattgt ttatatatat cacattaata agagttaatt
     1861 ttatttatta ctttagttgt atttgatgcg ttttaaataa attttatgag gtgaaatttt
     1921 ggaaaaggat ttttttagaa aaaatggaat tgatttagcc aaatcaatat taggtaaata
     1981 tctaataagg aaatatgaaa ataaagtaat tgttaccaaa atcatagaga cagaagcata
     2041 catgggtgta aatgataaag gagcacatgt atatggcaat aaaaagacag atagaactaa
     2101 gccattatat ttagatggtg gacatatata tgtttattta atttatggta tgtataattg
     2161 cttgaattta tctgcaaata tagaaaatgt gccagagtgt gtattaataa gaggggtaga
     2221 gcctataact tctttagatg agatatctat gaatagatac aacaaagctt acactgagtt
     2281 gagtaaatat caagtaaaaa atattactaa cggaccagga aaactttgca aagctttaaa
     2341 aatagataga agtctaaata gtaagagtat aatgggtgaa gaattatata taagtgattt
     2401 ttattatgat gataaaggaa aaaaagtatt tagtaaagat gagttagata taa
//
LOCUS       DACULQ010000294         2446 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.296, whole genome shotgun sequence.
ACCESSION   DACULQ010000294 DACULQ010000000
VERSION     DACULQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.296"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..854
                     /locus_tag="JA154_18205"
     CDS             <1..854
                     /locus_tag="JA154_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAT7997755.1"
                     /translation="IQNLFLGIYELKSESVIFENTLDDNKIIAKMDTYIIIDYINASF
                     EYLGYVLNGSALNSIDYGVPQNRERFVLIGAKKDFLKNKKIETPKPIVGENYVTVRDA
                     IGDLAEYEASKGSMDYCFEKNSNNIEKDFYRKIILDSNKIYNHVCTDTRNIALKRFEY
                     INQGNNFHSLPDELKGTYADPERTQNTIYKRLVYDKPSDTVVNVRKSMWIHPIKNRAV
                     SAREAARLQSFPDSYKFLGTKDSVYQQIGNAVPPLLGRVVAEKILNLFNDEPNEYLRD
                     VFEALDG"
     gene            complement(1854..2426)
                     /locus_tag="JA154_18210"
     CDS             complement(1854..2426)
                     /locus_tag="JA154_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HAT7997756.1"
                     /translation="MEKLVKILINIICIIALTFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNINYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNNNDEFKYSLNYIT"
BASE COUNT          908 a          301 c          327 g          910 t
ORIGIN      
        1 atattcaaaa tttattttta ggaatatatg agttaaaatc tgaatctgta attttcgaaa
       61 atacacttga tgataataaa attatagcta aaatggatac ttacattata atagattata
      121 taaatgcatc ctttgagtat ctaggatatg tattaaatgg aagtgcacta aattcaattg
      181 attatggagt acctcaaaat agagaaagat ttgtattgat aggagcaaaa aaagacttct
      241 taaaaaataa aaagatagaa actcctaagc ctatagttgg agaaaattat gttacagtaa
      301 gagatgcaat aggcgattta gctgaatatg aggcttctaa aggtagtatg gattattgtt
      361 ttgaaaaaaa tagtaataat attgaaaaag atttttatag aaagataata ttggatagca
      421 ataaaatata caatcatgta tgtacagata caagaaatat cgcactaaaa agatttgaat
      481 atataaatca aggaaataat tttcattctt tgccagatga gttaaaagga acttatgctg
      541 atccagaaag aactcaaaat actatttata aaagactagt atatgataag ccatcagata
      601 ctgttgtaaa tgtaagaaaa tctatgtgga tacatcctat taaaaacaga gctgttagcg
      661 caagagaggc ggctagattg cagtcattcc ctgatagtta taagttttta ggtacaaaag
      721 attctgtata tcaacagata ggaaatgcag taccaccttt attaggtcga gtagtagcag
      781 aaaagatatt aaatttattt aatgatgaac caaatgagta tcttagagat gtgtttgaag
      841 cactagatgg atagttcaaa taaaatagtg ggtattattc ttgtgattct tatccaacat
      901 taatccacga aataaggact gtcgaatgag taattttaaa ttgctgtgcg acagttcttt
      961 ttttacttgc ttttcattaa tttatattta tgcaaaattt gagcttgaaa acggtcataa
     1021 gaaataaagg tataaaaata caattttaaa atagaataat ttacaaagtg taagattaaa
     1081 aaatattttt gagaggatga atatacatac tacatttatt aatgattatc ttatttgtga
     1141 aacttagaaa tactataggc taacactaca ggtataaact ctgaaagaat attttgattg
     1201 cctttgatta gaattctttt aaagttcata ctatgtttta tttgggtgaa agctccttat
     1261 gcctgaatac ttctattcat tctaagttca catccttgtg taccagtgat tctttctaaa
     1321 ttatcttaat gccaaaatct tgttcgcatt tcaaaataaa gtcaggtagc tacttcaaca
     1381 ttttagttat atttcttcaa tagtttgtct ccaaacaaag gtacatctat tagaaacaga
     1441 tttaatttta tttatatcaa taaataatat atactttgaa acttcattaa tatcaccaag
     1501 ataatttata agttaagcta ttatatacta tcttcaccaa ctacatcctc catagtttta
     1561 cactttctta attcagcttg gtaatctaaa tctgtaataa ttatttctct ttgatttttc
     1621 atttctttgc ctacaaaagt ttaatataaa tattattcca tacttaccaa ttttacgttc
     1681 aaaaaagact gtagataagt ctgcatttac acaaacctac ttataggtct cttttttgaa
     1741 attaatttag catgaactat agtaagagca tctttaaatt catagttaca agtatacaaa
     1801 gttataattt tatctgaaat atctaccttt atatcagatt tgtgtactga tttttaggtt
     1861 atataattca aagaatattt aaactcatca ttattattaa agtgtatact taaataatct
     1921 ctgttaatta agtcagcagt atatactgaa aaaatttcat aagtataagt ttgtacttta
     1981 taatttattt ctactaagtt attttctttg aaaaatactt catcagtata gttttctagt
     2041 tgaccaaaca tagttttatt tttcatataa tgaccataga ctattaaact cttatcattt
     2101 tcaaaattat ttctataatc catgaaaata ctacctaaag gaagataatt tttataaaaa
     2161 tcatgagtta ggtagaaatc attgttatat ccttgtacta caggatagtt tatatttacg
     2221 ttgtttattt taagccagaa tctataatca ttatttttat cataaagttc ttgatgtttg
     2281 ctattattga tagtgttttc tctgagttcc gtatatactt catcagcttt tttatattca
     2341 gataatttta tgtaaatcga aaggctagag aaagttagcg caataatgca aataatattt
     2401 attaatattt ttactagttt ttcaattaat taccccttag tattat
//
LOCUS       DACULQ010000295         2402 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.297, whole genome shotgun sequence.
ACCESSION   DACULQ010000295 DACULQ010000000
VERSION     DACULQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.297"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            26..2161
                     /locus_tag="JA154_18215"
     CDS             26..2161
                     /locus_tag="JA154_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAT7997757.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1024 a          246 c          461 g          671 t
ORIGIN      
        1 atgaaaagta aaacaggagg gaaaaatgat accaggactt aaagaaagtt tgctaggatt
       61 aataaatgaa agtgcatata atcctctaaa aaaagaagaa ttagcagaaa tatttgatat
      121 acattcatca gaaatgccaa tgttttataa ttttttaaat gaattagaag aagatggtta
      181 tatctgcttt actaaaaaag gaaaaatagt atctcccaat caaatgggat actttgttgg
      241 aaaatttgta tcacacaaaa agggttttgg atttgtagaa tctgatgttg aatatacaca
      301 ggatttattt atctcaagtg acaatataaa tggtgctatg cataatgata gagttatggc
      361 agaaatagta gtacctgcaa ctgaagaaaa gagagcagaa ggaagaatag taaaagtaat
      421 aaagagagaa ataactagaa tagtaggtac cttccaatca agtaaaactt ttggatttgt
      481 aacaccagac aacaagaaat ttactaaaga tatatatata cctaaaaaac attttagtgg
      541 agcagtagat aatgataagg tagtatgtga aattacagta tggccacaag aggatagaaa
      601 accagaaggc aaaattatag agatactagg tcaaaaaggt gaaagaggcg tagaaataga
      661 ttctataata agagaacatg gtcttccaga agaatttcca aagaaagtat tgcaagaagc
      721 agaagctgta gctgttgaga ttcctcaaga agagataaaa agaagaagag atttaagaga
      781 tttaaacata tttactatag atggagatga cgcaaaagac ttagatgatg ccatatcaat
      841 agaagtatta cctaatggaa acttcaaact aggagttcac atagctgatg ttacacatta
      901 tgtaagagag aaaaataaac ttgacaagga agccttaaaa agagctacat cagtttattt
      961 agtagataaa gtaattccaa tgttgccaaa aactctttca aatggagtat gtagcttaaa
     1021 tccatttgaa gataagctaa ctctttctat atttatggaa atagaccata aaggaaatgt
     1081 ggttaaacat gaaatatgtg aatctgtaat aaattcaaaa gctagaatga cttatacaga
     1141 agtatctgat atattagaaa aggatgatga aaaacttaaa aagacttttg aacatgttgt
     1201 tgaagatttt aaaaatgctg aaattttagc taggattctt atgtcaagaa gggaaaaaag
     1261 aggagctata gacttcaatt tcccagaggc aaagataatt ctaaatggaa aaggcgaagt
     1321 tgtagatata aagccatatg aaagaagaat atctaataag ataatagaag aatttatgct
     1381 tataagtaat gagacaattg ccgaacattt ttactggatg ggaataccgt ttgtgtatag
     1441 aatacatgag acaccatctt tggaaaaaat ggaagaattg agtaagttta tatctacatt
     1501 tggatatata ataaaaggtg ataaagagga agtacatcca aaagcattac aaggtataat
     1561 agagaaaata aagggcaaaa aagaagaagg agctatcagt actataatgc ttcgttcttt
     1621 aaaacaagct aagtactcac cagaatgtag tggacatttt ggattagcag ctaaattcta
     1681 ctgtcacttt acttcaccaa taagaagata tcctgacctt caaattcata gaataataaa
     1741 agaatcttta aataataaaa taagtggaaa aagacaagaa cagcttacta ctatagtgga
     1801 ttatgcatct gttcaatcat ccgagaaaga aagaaaggca gagttagctg aaagagatgt
     1861 acatgatttc tataaggctc tttatatgaa ggataaagtt ggtcaagaat ttgagggtgt
     1921 tgtatctagc gtaacttcat ttggaatatt tgttgagtta gataatacca tagaagggtt
     1981 gataagactt gcaaatatga atgatgatta ctatatttat gatgagagtt cttatagtgt
     2041 tttaggagaa agaactaaga agtcatataa gataggtgac cttgttaaga taaaggttga
     2101 aagtgttaat gtagatttta aagaaattga ctttgagata gttgaaaaaa tagaggagta
     2161 gataaaacag ctaaaataag agataacaag ttgtagtatt tatgatatgt ataaatatat
     2221 agtgcacaat actctataaa ataaatgata aaaataggta attgagaaat atctaatgga
     2281 aatagttaac tgaaagataa ataattaaga aatatttaat gaaagtagct aattgaaaaa
     2341 taagtaattg agaaatgttt aatagaaaga gctaactgaa agataagtaa ttaagaaaca
     2401 tt
//
LOCUS       DACULQ010000296         2383 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.298, whole genome shotgun sequence.
ACCESSION   DACULQ010000296 DACULQ010000000
VERSION     DACULQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.298"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1211
                     /locus_tag="JA154_18220"
     CDS             <1..1211
                     /locus_tag="JA154_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAT7997758.1"
                     /translation="ARKESVNLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGI
                     WTKYCTDMEFLKNVFINRCIAFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNN
                     IKKEKKSISKIVFNLVLILLISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAP
                     ENTLAAIEEASHAKADYAEIDVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKT
                     YDAGSFYYYGKGYYDEKFVGEKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVV
                     QLIKENNFENQCIVASMDKTILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIES
                     TFVNKTVVEKIHKMNKKIFVWTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNG
                     FISQVSDYLF"
     gene            1533..2117
                     /locus_tag="JA154_18225"
     CDS             1533..2117
                     /locus_tag="JA154_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAT7997759.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSLDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT          925 a          227 c          392 g          839 t
ORIGIN      
        1 gagcaagaaa agaaagtgtt aatcttataa agggaaagat tataagaaca attatttatt
       61 cattaatatt atctttagta gtagctatta ttggatatgc gatttatcat agtggaatta
      121 tatttatagg catatggact aaatattgta ctgatatgga atttttgaag aatgtattta
      181 taaatagatg tattgcattt gaagaatatt ttagattgat tttatcaata ttcattttta
      241 tattggacat tggatttatc tcagcaactt attatcaata taaaggagta aatatttcta
      301 aaaataatat taaaaaagag aaaaaatcta tatcaaaaat tgtttttaat ttagtattaa
      361 tattattgat ttcttatata gagtcagttg cattttcttc acatataaat ggtatgttta
      421 atacgtcatt ttcattttat acaacagcaa ttgctcatag agcaggagct gaaatggctc
      481 cagagaatac tttggcagca atagaagaag cttcacatgc taaagctgat tatgcagaaa
      541 tagatgttca agaaactaaa gatggagaac ttattgtact tcatgattca aactttaaga
      601 gaactacagg agtagataaa aatgtttggg atgtaaatta taatgaagta aagacatatg
      661 atgcgggaag cttttattat tatggaaaag gttattatga tgaaaagttt gttggggaaa
      721 agataccaac actcaaagaa atgataaaat attctaaagg taaggtgaaa ttattaatag
      781 aaataaaact taatggacat gaaaagggag acgtagtaaa aaaagttgtt cagttaatta
      841 aagaaaataa ttttgaaaat caatgtatag ttgcatctat ggataaaact atattacaaa
      901 aagtaaaaaa actggatgag aatttactga catgctattt tatggcaatt gcatatggtg
      961 atttttcaaa gttaaattat gttgatatat atggaattga atcaactttt gttaataaaa
     1021 cagtagtaga aaagattcat aaaatgaaca aaaaaatctt cgtttggact gttaatagtg
     1081 acagtttaat tgagaaaatg cttgacctaa atgtagatag tattattaca gataatccat
     1141 accttataga gagtgcaatt tattggaaga aaaatggatt tattagtcaa gtgtcagatt
     1201 atttatttta aatattctat aattgtatta tccagtaaaa gagatattat taaaatcaat
     1261 aatatctctt tttaacataa ataaggttgt taaattaagg ctattcttat aaaaattgtg
     1321 tttatcagat ataaataagg aataaaagta aaaaaattgc tatgacatta ttacaattaa
     1381 aagagtttta atatgataga gaggtagatt gtcaactgta tatttaataa attttattat
     1441 ttattagatt ttatataagt aaaatatata gatatataaa tcttatgcta gaatatatat
     1501 gaaaaacaat atagatttgg agggttaaaa gcatgacaga gcgagaaaaa atgttggcag
     1561 gagaacttta cgattgtggt gatattgagt tgttaactca atggcataaa gcaaaagacc
     1621 tagtaagaga ttataaccaa acagattcct tggatttgga aaaaaaagaa aaaatattaa
     1681 atgagttact tggtggtaag ggtaaaaact tatggattac tgcaccgtat tatgtagatt
     1741 atggaaataa tatttatttt ggaaataatt gtgaagtcaa tatgaattgt acgtttttag
     1801 atgataataa aattatcatt ggggataatg cactaatcgc accaaacgta caaatatata
     1861 ctgcatttca tccaactaat gctggtgata gatttggtaa tgccaaagaa gatggttcat
     1921 ttgaattttg taaaactcaa acagctccag ttactattgg agataatgtt tggattggcg
     1981 ggggtgctat tatcatgcca ggtgttacta ttggagataa tgtagttata ggtgcaggaa
     2041 gtattgttac aaaagatatt ccaagtaata tgattgcata cggaaatcca tgccgtatta
     2101 ttcgagaaaa taaataaagt taaattttct tgcaaaatct caaaaaacac ctttcttact
     2161 ttatacatag ggtattttct aatactttca catccatatt catttgttaa atttttgtgg
     2221 aatgtagttt ttatcacttc tatcttacat ttccaccatt tttttataaa agtcattcct
     2281 ttatagattt atttataata gtatatattc tatttgatgt ttgaataagc acttattttt
     2341 aagtaaaata ctaacttcta ggttagtatt ttactatagt atg
//
LOCUS       DACULQ010000297         2370 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.299, whole genome shotgun sequence.
ACCESSION   DACULQ010000297 DACULQ010000000
VERSION     DACULQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.299"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..146)
                     /locus_tag="JA154_18230"
     CDS             complement(<1..146)
                     /locus_tag="JA154_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997760.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNI"
     gene            complement(299..1258)
                     /locus_tag="JA154_18235"
     CDS             complement(299..1258)
                     /locus_tag="JA154_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine sulfoxide reductase"
                     /protein_id="HAT7997761.1"
                     /translation="MKMIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTY
                     QEVCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFY
                     HDEEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYY
                     KGSGRYNFVKSHWDGNNINREELKKTLTHIQFEVTQNDMTEVPFENEYYDNNKEGIYV
                     DIVSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLG
                     HVFDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            complement(1376..2299)
                     /locus_tag="JA154_18240"
     CDS             complement(1376..2299)
                     /locus_tag="JA154_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HAT7997762.1"
                     /translation="MWGYMYMKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNN
                     INMFGGTSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKE
                     VLLSIFPEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMAS
                     SAAPVFFPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKAD
                     TTEWGAIDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDD
                     CDALEYLMSLGENCDIKDAFGWIKKKSFYRN"
BASE COUNT          762 a          433 c          235 g          940 t
ORIGIN      
        1 tctatatttg gtacatactt attttcctca aaagctttta aatctttttt atttatttta
       61 gtttctttag acaactgttc tatcgtcttt gctctaaaaa ttctagctga ttttaatctc
      121 tttccattaa agtcttttaa attcatttgt tttctctctt tcaaaattaa attcttaatc
      181 tattggtata aataagatta caattaataa tttatcatat tattgacaaa aaaacaaaag
      241 gtgaaaatat ttttttatat tttcaccttt tagataagtt tataataaat attcctaatt
      301 actcaaaaag ttttaaatat tctccatatc ctaaagcttc catattttct cttggtataa
      361 atttaagaga agcagaattt atacaatatc ttaaacctcc aagttctttt ggaccatcat
      421 caaatacatg acctaaatga gaatttcctt tagcacttct tacttcaact ctgtacatac
      481 cttgtgagaa atcacttctc tcaatgatag atttatctat aactggcttt gaaaaacttg
      541 gccacccaca tccagaatca aatttatctt ttgaagaaaa taacacttct ccacttacaa
      601 tatcaacata aattccctct ttgttattgt cataatattc attttcaaaa ggtacttctg
      661 tcatatcatt ttgagtgact tcaaattgta tatgagttaa agtctttttt aactcttccc
      721 tatttatatt gtttccatcc caatggcttt ttacaaaatt atatcttcca gaccctttat
      781 aatacatatt gtagtttaat ggtcttttct tataataatc ctgatgattt tcttctgctt
      841 cgtagaattt agttgctgga attatatcag tcactatttt actatcaaat attttactat
      901 tttccagatg ttctcttgac tctaaagcta tatttttttg ttcttcatca tgataaaata
      961 tagcagtctt atattgagtt cccctatcac caaattgtcc accatcatct gtaggattaa
     1021 tctgtttcca gaagatattt agaagttcat cataacttat tatatcatca tcatatgtta
     1081 tttgaattgc ttctaaatga ccagtagttc ctgagcatac ctcttgatat gtagggtttt
     1141 caacatgacc acctgtatat ccagaaacta ctttaataac tccatcatac ttatcaaaag
     1201 gttttaccat acaccaaaaa catccacctg caaaagtagc aagttttatc attttcatca
     1261 ctccttaaat aaattatact ttatttattc cctaagaagt aacttaataa tctgcatttt
     1321 attattttta cctactcaat gaaatcatgt gtaaaaatac aattgcaaat tgaatctaat
     1381 ttctataaaa gctctttttc tttatccatc caaaagcatc ttttatatca caattttctc
     1441 ctaaagacat tagatattct aatgcatcac aatcatccat agctacatct ttttccatct
     1501 taggatttaa tctatagtaa ttatctccaa gaagtttttg agaaaataac tgactcattt
     1561 gagaatttcc ttctaaggta atagatataa ttggtaaatc gggttcttta ttaattatcc
     1621 agtcaatagc tccccattca gtagtatctg cctttatact gttatataca tatccagtcc
     1681 ctattgaaaa aagtcttgta ttcttaagtt taaatccact gtcaatggca tgaattatac
     1741 acgctaaact cgggtcagtt gctattattc ctccatcaac atgacgatta tatgttggaa
     1801 aaaataccgg agcagcacta cttgccattg ctacatctac aactttgcta gtctttgtaa
     1861 aagaatttgg catattgtta taaaacactg gtttccatgc attttcctca tttcctatat
     1921 aaaaacttgg tatcattact aatttattta aatcttttaa ttctagttcc tctggaaata
     1981 tacttaataa aacttctttt aagtttttgt tttcatactt tggtctgagt atttctgcat
     2041 aacttttatt aaaaatatac tttgaatttt caattgagta aagttctttt atctctttag
     2101 ggcttacacc atacgcaagc cctagtgcaa ttagactccc tgtagatgta cctccaaaca
     2161 tatttatatt gtttaataga tttggatact cttttactat cctctcaagt atggatatac
     2221 tcaatgcccc ttttaatcca ccaccatcaa atgccaatat attaaaatta gaatttttca
     2281 tatacatata cccccacata aaacacctta tattttaaat tttaatcaaa ataatttcac
     2341 tatagtatat atattttatg caagttacat
//
LOCUS       DACULQ010000298         2311 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.300, whole genome shotgun sequence.
ACCESSION   DACULQ010000298 DACULQ010000000
VERSION     DACULQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.300"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            216..560
                     /locus_tag="JA154_18245"
     CDS             216..560
                     /locus_tag="JA154_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAT7997763.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPVRFDVIEVYL
                     LEKRINHIMNAF"
     gene            591..2192
                     /locus_tag="JA154_18250"
     CDS             591..2192
                     /locus_tag="JA154_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HAT7997764.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
BASE COUNT         1012 a          204 c          413 g          682 t
ORIGIN      
        1 cgtataaaag caactaattt ttctctgttc attaaaggca ctcctccaaa aaaataaact
       61 tttactatga aaactagaat aatatcataa aagaataaag tcaataaaag tatttattaa
      121 tattgatgga aataaaatca atccaaaaac ttgaatagta aaattgtata ctgacaataa
      181 tttaaaagtg aaaatttaat gtattggagt gaataatgaa taataaagaa aaaggtgatt
      241 ttggagaaaa agtagcagtt aattatttgc tatcaaaagg agcaaagata ctagaaaaaa
      301 attacagatt aaagataggg gaaatagata taatagcaaa aatggaagat gaaattatat
      361 ttgtggaggt gaaatcaagg tctaacataa agtttggcta cccttgtgaa tctgtaagct
      421 tcaaaaaaag aaaaaagata attggagttg caaattatta tattataaaa aataacttaa
      481 ataacactcc cgtaagattt gatgttatag aggtatattt attagaaaaa agaataaatc
      541 acataatgaa tgctttttaa aaaagataat gtttttggaa aggattaaat atgttaagta
      601 ttattaattc aagcaactta gtaggaatcg acagcttttt agttaaggtt gaagtagatg
      661 taagcaatgg aatacctagt tttaatattg ttggtcttcc aggcaaagaa attaaagaag
      721 caagagaaag agtaaagtca gcaattctta atagtggata taaatttcca agcacgagaa
      781 tagttgtaaa tttatcacca gcagatataa aaaaagaagg agctttttta gatttatcta
      841 taagtatagg ccttttaaga gagttaataa aaaaagatga aaattacata agagaaagta
      901 tgtttatagg agaattatca ttagatggga aaataagaaa agtaagaggt atattgccca
      961 taattatggg tgcaaaaact cagaacataa aacgtatttt tataccaatt gagaacataa
     1021 aagaaagtct atttgtagat gaaattgata taattcctat taaaagttta aaagaatgtg
     1081 tcgatttttt aaacgaagag ataaaagtcg ataaagtaag gataatgtct tttttggatg
     1141 ataagtcaag aaaagagaac gaagaattag aaaaagataa tagttatata gattgcaaat
     1201 atactaagat taataatgaa gaaagtaaat atgatgaaga ctttaaagat gtaaaaggaa
     1261 attattttgt aaagaggagt gcagaaattg ctgctgctgg aaatcataat atgtttatga
     1321 ttggacctcc aggctcagga aaaactatga ttgcaaaaag agtaaggaca attttaccag
     1381 atataagtgt agaagaaatg atagaagtaa gtaaggtcta tagtatattg ggtatgataa
     1441 atgaaactaa aggaataatt gataaaagac cttttagagc acctcatcat acaactacaa
     1501 agcaatcttt aattggagga ggaatggatg caagacctgg agaaattgct ttagcacata
     1561 gaggtatctt atttttagat gagatagccg aatttgatag aaaaatactt gagactttaa
     1621 gacaacctat agaagatgga tatgtaaata tatccagagt aaagtacagt gcaaaatatc
     1681 cttgtagagt actgttagtt gcagctatga atccatgtcc atgtggttat tatatgtcag
     1741 agacagagtg tagatgtaga agtaacgaaa ttgatagata cataaataaa atatcaggtc
     1801 cattgttaga taggtttgat atttttgtag aagttaattc tataaaatat tcagatttta
     1861 attctttaaa acaagaagaa agctctcaaa aaataaagag aagagtagaa aatgctagaa
     1921 aaatacagat aaatagattt aagaaggata atataaaaaa taatagtgaa attaaagctt
     1981 acaatttatt taaatattgt aaactggaaa aagaagcttc aaaaacggct gagatgatat
     2041 ttaataaata caacctaagc agtagaagtt atacaaaact tttaaaaatg gcaagaacaa
     2101 tagcagattt agaagaaaga gacttgataa attcacagtg tattatagaa gctttttcat
     2161 ttagaaaggc ttattatagt tattttaaat agttttttga ggtggatttt atggaaaaaa
     2221 gagatactta tttatggtta aaatcaattg gtggaataac aacaaaaaca attgaaataa
     2281 tagaaaatga aatagtaaat attgaagata t
//
LOCUS       DACULQ010000299         2225 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.301, whole genome shotgun sequence.
ACCESSION   DACULQ010000299 DACULQ010000000
VERSION     DACULQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.301"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(327..878)
                     /locus_tag="JA154_18255"
     CDS             complement(327..878)
                     /locus_tag="JA154_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7997765.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1069..2031
                     /locus_tag="JA154_18260"
     CDS             1069..2031
                     /locus_tag="JA154_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HAT7997766.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT          837 a          248 c          379 g          761 t
ORIGIN      
        1 cactccaatt gtgagaaata catacacaaa tcattattac aacaatgaaa aatgtacata
       61 taagagacgt tttgtatttg ttttaagtga taatatttta atactaacta aataattatc
      121 acaatggtaa aaaagaaaaa ggagtatttt gtactccatt tgtcaaaaat ataaaacttt
      181 tatataagct tattataaca taattgataa aagtgtaaag atatataatg aatttataaa
      241 gaaaaataaa ataaaaaaga taaatcctaa tcaaaggatt tatctttttg tatagaaaat
      301 aattattcta ttattaaatg gcatggctaa actggataat aatttattat tactgctttc
      361 aatgtttttt ctccttgatt atggtatgta tgaattgatt cagctgaaaa acaaatagag
      421 tcattttctc taagttgata aatagagtca tttacttcta attttaattg accttctata
      481 atcataatat attcctgtga tttttcagaa tgaccaacag atgtataact atgaccttct
      541 tctaattcca tttgaaataa ttcgaagttt ctagttggtg tattgggata ataacaataa
      601 agacgataat ctttattttc tgaaacttgt acatctatat cagtttttga tacaataaaa
      661 gtttcatttt gaggttgttc cagtattgca gtatatggaa catttagtcc acttgcaatt
      721 ttccaaattg tgtttactgt tgggtttgag tctccctttt caatttgaga aagcattact
      781 ttacttacgc cagataattc agcaagttga cctagactta agttacgttc attacgtaat
      841 ctatttaaat ttttagcaat aacagtatta atactcatag tagctcctta aaatgtaaat
      901 tattgacaaa tataatttac gaatgtataa tttatttaac attatttata atttacaaat
      961 gtataatata atgaacgtta aatacactgt acatccgtaa tataaattat actgcataaa
     1021 gagttgtttt tcaacggatg atagttgaga gattaagggg gaattgtaat gcgtgtactt
     1081 aataatgtta cagaggctat aggcaatact catatgttac atttatccaa aatagcagat
     1141 gaatttggag ttcaaggtaa tatttatgca aagatggaat acttaaatcc gggatttagt
     1201 aaaaaggacc gtgtagcatt acaaatgatt accgaagcag aaaatgcagg tttattagtt
     1261 ccaggtcaag cagttgtgga attaacaagt ggtaatactg gaacaggact agcaatagct
     1321 tgtgcatgta aaggatataa atttatagcg tgtatgtcaa aaggaaattc tatggaaaga
     1381 gcaagaatga tgaaagcttt aggtgctgaa gtagttcttg tagaccaaat gcctaattca
     1441 gttcatggac aggtatctgg tgaagatttg gcacttgtag aaattgaggc tcaaaaaata
     1501 gcaaaagaaa gaaatgcatt tagagcagac caatttaatt tagaagcttg taatcatgca
     1561 catgaaaagt atacagcaga agaaatgtgg gaacaattgg atggaaatat tgatgtattt
     1621 gtagattttc ttggaagtgg tagtactttt gcaggatgtt caaaaacact taaaaaacat
     1681 aattcttcta tcaaatgtta tgttgttgaa cctgaaacag cagcaattta ttcaggtaaa
     1741 gaaataactg accaagggca taaaatacaa ggtggaggat attctatgga tttgccatta
     1801 gtagacaaga aacttattga tggttatatt caagttacag acgaggaggc aactgatgta
     1861 gcaagaaaat tagctaaatt agaaggtgta tttgcaggat tttcttcagg ggcaaatgta
     1921 tgtgcagcaa ttaagttgtt aaagagcagt gaaaaaggaa aaaatatagt tttaactctt
     1981 aatgatagtg gtttaaaata tttaagtact gatttatatg aagaattgta ataatagcac
     2041 aatagttatg caatgttttt taaattaagt atatagattt aaattataaa aagtatctca
     2101 atgtaaattt tgaaagttat aaaggagata cttttttatt ttgtaaatat ggagtatcct
     2161 acttataagt attaagctat atagatattg atgattagct atgcaataaa aaagagtatg
     2221 tgttt
//
LOCUS       DACULQ010000300         2196 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.302, whole genome shotgun sequence.
ACCESSION   DACULQ010000300 DACULQ010000000
VERSION     DACULQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.302"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="JA154_18265"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="JA154_18265"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..365
                     /locus_tag="JA154_18270"
     tRNA            291..365
                     /locus_tag="JA154_18270"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
     gene            372..457
                     /locus_tag="JA154_18275"
     tRNA            372..457
                     /locus_tag="JA154_18275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:406..408,aa:Leu,seq:taa)
     gene            473..548
                     /locus_tag="JA154_18280"
     tRNA            473..548
                     /locus_tag="JA154_18280"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:506..508,aa:Met,seq:cat)
     gene            556..630
                     /locus_tag="JA154_18285"
     tRNA            556..630
                     /locus_tag="JA154_18285"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:589..591,aa:Glu,seq:ttc)
     gene            640..713
                     /locus_tag="JA154_18290"
     tRNA            640..713
                     /locus_tag="JA154_18290"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:672..674,aa:Gly,seq:tcc)
     gene            719..794
                     /locus_tag="JA154_18295"
     tRNA            719..794
                     /locus_tag="JA154_18295"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:752..754,aa:Val,seq:tac)
     gene            800..876
                     /locus_tag="JA154_18300"
     tRNA            800..876
                     /locus_tag="JA154_18300"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:834..836,aa:Asp,seq:gtc)
     gene            886..960
                     /locus_tag="JA154_18305"
     tRNA            886..960
                     /locus_tag="JA154_18305"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:918..920,aa:Thr,seq:tgt)
     gene            975..1059
                     /locus_tag="JA154_18310"
     tRNA            975..1059
                     /locus_tag="JA154_18310"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1009..1011,aa:Tyr,seq:gta)
     gene            1069..1152
                     /locus_tag="JA154_18315"
     tRNA            1069..1152
                     /locus_tag="JA154_18315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1103..1105,aa:Leu,seq:tag)
     gene            1182..1258
                     /locus_tag="JA154_18320"
     tRNA            1182..1258
                     /locus_tag="JA154_18320"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1216..1218,aa:Arg,seq:tct)
     gene            1266..1341
                     /locus_tag="JA154_18325"
     tRNA            1266..1341
                     /locus_tag="JA154_18325"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1299..1301,aa:Gln,seq:ttg)
     gene            1430..1518
                     /locus_tag="JA154_18330"
     tRNA            1430..1518
                     /locus_tag="JA154_18330"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1464..1466,aa:Ser,seq:tga)
     gene            1522..1597
                     /locus_tag="JA154_18335"
     tRNA            1522..1597
                     /locus_tag="JA154_18335"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1555..1557,aa:Phe,seq:gaa)
     gene            1604..1677
                     /locus_tag="JA154_18340"
     tRNA            1604..1677
                     /locus_tag="JA154_18340"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Met,seq:cat)
     gene            1692..1768
                     /locus_tag="JA154_18345"
     tRNA            1692..1768
                     /locus_tag="JA154_18345"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1726..1728,aa:Ile,seq:cat)
     gene            1792..1868
                     /locus_tag="JA154_18350"
     tRNA            1792..1868
                     /locus_tag="JA154_18350"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1826..1828,aa:Pro,seq:tgg)
     gene            1876..1952
                     /locus_tag="JA154_18355"
     tRNA            1876..1952
                     /locus_tag="JA154_18355"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1910..1912,aa:His,seq:gtg)
     gene            1961..2036
                     /locus_tag="JA154_18360"
     tRNA            1961..2036
                     /locus_tag="JA154_18360"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1994..1996,aa:Lys,seq:ttt)
     gene            2044..2117
                     /locus_tag="JA154_18365"
     tRNA            2044..2117
                     /locus_tag="JA154_18365"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2076..2078,aa:Cys,seq:gca)
     gene            2124..2195
                     /locus_tag="JA154_18370"
     tRNA            2124..2195
                     /locus_tag="JA154_18370"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2156..2158,aa:Asn,seq:gtt)
BASE COUNT          554 a          501 c          570 g          571 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agccaaaaaa agccgaagtg gcggaactgg cagacgcaca ggacttaaaa tcctgcggga
      421 cttacctctc gtaccggttc gattccggtc ttcggcactt ataaaacaat atcgcggagt
      481 ggagcagttg gcagctcgtc gggctcataa cccgaaggtc gtaggttcaa gtcctgcctc
      541 cgcaaccatt aaaatggctc gttggtcaag aggttaagac accgcccttt cacggcggta
      601 acaggggttc aattccccta cgagtcacca attaaatatg cgggtgtagc tcaatggtag
      661 agttctggcc ttccaagcca gctgtgaggg ttcgatcccc ttcacccgct ccaaaaatgg
      721 gaccatagct cagctgggag agcacctgcc ttacaagcag ggggtcacag gttcgagccc
      781 tgttggtccc accatttgcg gcctggtagt tcagttggtt agaatgccag cctgtcacgc
      841 tggaggtcga gggttcgaac cccttccagg tcgccaaaat aaaatgctgg tgtggctcaa
      901 cggtagagca gctgacttgt aatcagcagg ttgtaggttc gattcctatc accagctcca
      961 tgtaaaatta atacggagga tttcccgagc ggccaaaggg ggcagactgt aaatctgttg
     1021 tcatcgactt cggtggttcg aatccaccat cctccaccaa aaaaatgcgc ggatgtggcg
     1081 gaattggcag acgcactaga cttaggatct agcgccattg gcgtgggggt tcgactccct
     1141 tcatccgcac catttttgtt tgatatacat atatatattg tgggtgcata gctcagctgg
     1201 atagagcaac gcccttctaa ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca
     1261 tatattgggg attagccaag tcggtaaggc acatgacttt gactcatgta tgcgtaggtt
     1321 cgagtcctgc atccccagcc aaatgatgta ttactaagct atatttgaat tatttttatt
     1381 ttaaatatag ttaggttcaa ttttagaata agtttaagta tccgaatgtg gagaggtgtc
     1441 cgagtggttt aaggagctgg tcttgaaaac cagtgacttc gaaaggggcc gtgggttcga
     1501 atcccaccct ctccgccaaa tgcccagata gctcagtcgg tagagcaggg gactgaaaat
     1561 ccccgtgtcg gtggttcgat tccgcctctg ggcaccaaaa tatggcggta tagcttagtt
     1621 ggctagagcg ttcggttcat acccgaaagg tcataggttc gactcctatt accgctacta
     1681 aaatttaatg tgggccatta gctcagttgg ttagagcgcc cggctcataa ccggtaggtc
     1741 tggggttcga gtccctgatg gcccaccaca ttaaattaat taatataaat tcgaggtgta
     1801 gcgcagtttg gtagcgcaca tggtttggga ccatggggcc gggggttcga gtcccttcac
     1861 ctcgaccaat ataaggtggg tatagctcag ttggttagag cgccagattg tggctctgga
     1921 ggtcgtgagt tcgactctca ttatccaccc caattcatat ggtctattag ctcagtcggt
     1981 agagcacctg acttttaatc agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa
     2041 ggtggcgaca tagccaagtg gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa
     2101 tctgggtgtc gcctccaaaa tattccaagg tagctcaacg gtggagcaac cggctgttaa
     2161 ccggtaggct gtgggttcga gccccaccct tggagc
//
LOCUS       DACULQ010000301         2164 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.303, whole genome shotgun sequence.
ACCESSION   DACULQ010000301 DACULQ010000000
VERSION     DACULQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.303"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(400..684)
                     /locus_tag="JA154_18375"
     CDS             complement(400..684)
                     /locus_tag="JA154_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997767.1"
                     /translation="MVLEQGYTKFSYEFETTEKDSSNINIALIDSDTIYLDNLSITEL
                     NSTPEILDEPEVKIPTVQEIIVTHKIYSADFKFNPSTIKAYINGMYFTPT"
     gene            complement(738..1088)
                     /locus_tag="JA154_18380"
     CDS             complement(738..1088)
                     /locus_tag="JA154_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997768.1"
                     /translation="MLYFNNIPIDESCVELIFDDNIGNKIKDSLKTLSDKQICNVKLE
                     RGMNILIKTPTYFTNFDDYNNYPSIWSNVNTTNQDGLKGSANRLNGETKIKIPMSELK
                     PYKRYVFSKIQKIL"
     gene            complement(1377..1568)
                     /locus_tag="JA154_18385"
     CDS             complement(1377..1568)
                     /locus_tag="JA154_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997769.1"
                     /translation="MYKVTPTTNLVLDGDALSTIKAQENQIGNNLSPGNTYLKKGFSP
                     MALNTMYQFSSRLIPINYN"
BASE COUNT          671 a          298 c          249 g          946 t
ORIGIN      
        1 ctttttaata caatatagtt ctaaaaaaga atatattaga agaagataat atttaaataa
       61 aaaatacctt ataaagttaa ttttttaata ctttataagg taaatacata ttgtattgtg
      121 gggataaatt taaatcattt gatactaatc aacactaaga accaataact ctctatcatc
      181 ttgagtaatt gtatatattc ttaatttctt gtatgtcata atattttctc cacctgtaaa
      241 ataactccta agattaacac aatcagtttt aggttgattt gtatctccta aataattacg
      301 caattcttta tctttagttt caatatcttt aaatccaaaa tattgtaaag tagcttcaac
      361 tctatatttt tggacataat cgagagcttc tttattagtt tatgttggtg taaaatacat
      421 accatttata taagccttta ttgtacttgg attaaattta aaatctgcag aatatatttt
      481 atgtgtaact attatttctt gaacagttgg aattttaact tctggttcat caagtatttc
      541 aggagtacta tttagctctg taatagataa gttatctaag tatattgtat cactatcaat
      601 taatgctata tttatattag aagaatcttt ttcagtagtt tcaaattcat aactaaattt
      661 tgtatatcct tgttctagta ccaaataatc cgttttttct tcttttactt ttatatttac
      721 aattattgaa ttatatgtta aaggattttt tgaattttac taaaaacata acgtttataa
      781 ggttttagtt cagacatagg tattttaatc ttcgtctcac catttaatct atttgctgag
      841 ccttttaaac catcttgatt tgtagtatta acattactcc atatactagg gtaattattg
      901 taatcatcaa aattagtaaa gtaagttggt gtttttataa gtatattcat tcctctttca
      961 agtttaacat tacatatttg tttatcagac aaagttttta aactgtcttt aatcttattg
     1021 cctatattat catcaaatat gagttcaaca caactttcat ctattggtat attattaaaa
     1081 tataacaaac catctttttt agtagtgcca aaagtttttt caattgcttc tccaattgta
     1141 agttcaggtg ttttatcttc tgggacctgt aaacttttag cagtaattct tcttttgtaa
     1201 gattcatttt ctgtatctaa tataatagat acagaaatac tgtcaatttg acctatatcg
     1261 tcttaccaac tatttcattc tgttactatt tgtccagaac cattttttgt accaaaattt
     1321 ccacttactt gtgttgtttc taatttaatt tgctttccat catctaattt ttttaattaa
     1381 ttataattta taggaatcag tctagaacta aattgataca ttgtgttaag agccataggt
     1441 gaaaatcctt ttttgagata agtatttcca ggagatagat tattgccaat ttgattttct
     1501 tgtgctttga tagttgataa tgcatctcca tctaacacta aatttgttgt tggtgtcact
     1561 ttgtacatag gtgcagtacc tgtgttgtag tatctaatat ttgcatttat atatgcagat
     1621 tctcctttgt ttatacttaa tccagtattt catgattatc cattgctatt ttgaacagca
     1681 gttgaattat ctgttgtatg gtaataattt gtcttgtata gtttctttaa actcattaaa
     1741 tttttctaat gaaatttcgt tttgattttt tgttcttatt actttattaa atgcaaattt
     1801 ttctggagat tcaaaataat atacatacat aggtttatct attttatttt ttgatatagc
     1861 atttcgaagt tctctatatt ctttttctca agaatttgct tctatttgat aatcataaaa
     1921 ataatttctt gtctgagaat atttgcttat ctctatagaa tcttttttat atgatcctaa
     1981 tacttctttt tcagattttt caagtttttg atcttttctt tctcattctt tagccttttc
     2041 tttatcttta aaaaaatcct tctgatcttt ctataggagc cttgtaagta gtattacaaa
     2101 cttttccact atagctttgt aaatcttgtg cataaatgtt attaggtaac aaactaccta
     2161 aagt
//
LOCUS       DACULQ010000302         2162 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.304, whole genome shotgun sequence.
ACCESSION   DACULQ010000302 DACULQ010000000
VERSION     DACULQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.304"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..132
                     /locus_tag="JA154_18390"
     rRNA            <1..132
                     /locus_tag="JA154_18390"
                     /product="23S ribosomal RNA"
     gene            260..376
                     /gene="rrf"
                     /locus_tag="JA154_18395"
     rRNA            260..376
                     /gene="rrf"
                     /locus_tag="JA154_18395"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            383..457
                     /locus_tag="JA154_18400"
     tRNA            383..457
                     /locus_tag="JA154_18400"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:415..417,aa:Asn,seq:gtt)
     gene            462..536
                     /locus_tag="JA154_18405"
     tRNA            462..536
                     /locus_tag="JA154_18405"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:495..497,aa:Glu,seq:ttc)
     gene            542..617
                     /locus_tag="JA154_18410"
     tRNA            542..617
                     /locus_tag="JA154_18410"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:575..577,aa:Val,seq:tac)
     gene            623..699
                     /locus_tag="JA154_18415"
     tRNA            623..699
                     /locus_tag="JA154_18415"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:657..659,aa:Asp,seq:gtc)
     gene            710..784
                     /locus_tag="JA154_18420"
     tRNA            710..784
                     /locus_tag="JA154_18420"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:742..744,aa:Thr,seq:tgt)
     gene            799..883
                     /locus_tag="JA154_18425"
     tRNA            799..883
                     /locus_tag="JA154_18425"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:833..835,aa:Tyr,seq:gta)
     gene            893..966
                     /locus_tag="JA154_18430"
     tRNA            893..966
                     /locus_tag="JA154_18430"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:925..927,aa:Gly,seq:tcc)
     gene            977..1053
                     /locus_tag="JA154_18435"
     tRNA            977..1053
                     /locus_tag="JA154_18435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1011..1013,aa:Arg,seq:tct)
     gene            1063..1138
                     /locus_tag="JA154_18440"
     tRNA            1063..1138
                     /locus_tag="JA154_18440"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1096..1098,aa:Gln,seq:ttg)
     gene            1150..1225
                     /locus_tag="JA154_18445"
     tRNA            1150..1225
                     /locus_tag="JA154_18445"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1183..1185,aa:Lys,seq:ttt)
     gene            1228..1316
                     /locus_tag="JA154_18450"
     tRNA            1228..1316
                     /locus_tag="JA154_18450"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1262..1264,aa:Ser,seq:tga)
     gene            1334..1424
                     /locus_tag="JA154_18455"
     tRNA            1334..1424
                     /locus_tag="JA154_18455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1368..1370,aa:Ser,seq:gct)
     gene            1433..1509
                     /locus_tag="JA154_18460"
     tRNA            1433..1509
                     /locus_tag="JA154_18460"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1467..1469,aa:Pro,seq:tgg)
     gene            1595..1670
                     /locus_tag="JA154_18465"
     tRNA            1595..1670
                     /locus_tag="JA154_18465"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1628..1630,aa:Trp,seq:cca)
     gene            1731..1807
                     /locus_tag="JA154_18470"
     tRNA            1731..1807
                     /locus_tag="JA154_18470"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1765..1767,aa:Pro,seq:tgg)
     gene            1814..1890
                     /locus_tag="JA154_18475"
     tRNA            1814..1890
                     /locus_tag="JA154_18475"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1848..1850,aa:Ile,seq:gat)
     gene            1894..1969
                     /locus_tag="JA154_18480"
     tRNA            1894..1969
                     /locus_tag="JA154_18480"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1927..1929,aa:Phe,seq:gaa)
     gene            1977..2053
                     /locus_tag="JA154_18485"
     tRNA            1977..2053
                     /locus_tag="JA154_18485"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2011..2013,aa:Met,seq:cat)
BASE COUNT          572 a          480 c          570 g          540 t
ORIGIN      
        1 caacttcaag ataagatttc ccaccgcaag ggtaagaccc cagaaagact atctggttga
       61 taggtcgaag gtgtaagtgc agcaatgtat ttagcttatc gatactaata ggtcgaggac
      121 ttgaccaata ttatttgatg tcattcatta agatatatgt gcagttttta gagtgttaac
      181 tctaaaaaaa aataaaaaaa gtattgacaa aaatagtaaa aaatgttaat attatacttg
      241 tcctagcaac aagaaaaaat gtggttataa tagcggagga gatacacctg tttccattcc
      301 gaacacagaa gttaagttct ctagcgctga tggtacttgg tgggaaactg cctgggagag
      361 taggacatag ccacgtgaat gttccaaggt agctcaacgg tggagcaacc ggctgttaac
      421 cggtaggctg tgggttcgag ccccaccctt ggagccatta tggctcgttg gtcaagaggt
      481 taagacaccg ccctttcacg gcggtaacag gggttcaatt cccctacgag tcaccatttg
      541 tgggatcata gctcagctgg gagagcacct gccttacaag cagggggtca caggttcgag
      601 ccctgttggt cccaccattt gcggcctggt agttcagttg gttagaatgc cagcctgtca
      661 cgctggaggt cgagggttcg agccccttcc aggtcgccaa aataaaaatg ctggtgtggc
      721 tcaacggtag agcagctgac ttgtaatcag caggttgtag gttcgattcc tatcaccagc
      781 tccatgtaaa actaatacgg aggatttccc gagcggccaa agggggcaga ctgtaaatct
      841 gttgtcatcg acttcggtgg ttcgaatcca ccatcctcca ccaattaaat atgcgggtgt
      901 agctcaatgg tagagttctg gccttccaag ccagctgtga gggttcgatc cccttcaccc
      961 gctccaaaga aagcgtgggt gcatagctca gctggataga gcaacgccct tctaaggcgt
     1021 gtgtccgggg ttcgaatccc tgtgcgctca ccacatatat attggggatt agccaagtcg
     1081 gtaaggcaca tgactttgac tcatgtatgc gtaggttcga gtcctgcatc cccagccaaa
     1141 taaaaatatg gtctattagc tcagtcggta gagcacctga cttttaatca gggtgtcccg
     1201 cgttcgagtc gcggatagat caccaatgga gaggtgtccg agtggtttaa ggagctggtc
     1261 ttgaaaacca gtgacttcga aaggggccgt gggttcgaat cccaccctct ccgccaaaaa
     1321 aatgactgaa tttggagaag tactcaagtg gctcaagagg atcccctgct aagggattag
     1381 gctgggtaac cggtgcgagg gttcgaatcc ctccttctcc gccattcaaa aacgaggtgt
     1441 agcgcagttt ggtagcgcac atggtttggg accatggggc cgggggttcg agtcccttca
     1501 cctcgaccaa cttaagcttt tagttcagtt ggttagagtg cccaactcat ggctagtaat
     1561 tctagggttt gagtccatga aggttattct tcataggggt gtagctcagt tggtagagcg
     1621 tcggtctcca aaaccgtgcg ccgggggttc gagtccctcc acccctgcca aataaaaagg
     1681 acttgaaaaa agttggattt tagagtagta taagatagta aataagattt cgaggtgtag
     1741 cgcagtttgg tagcgcacat ggtttgggac catggggccg ggggttcgag tcccttcacc
     1801 tcgaccaaga aaagggccta tagctcaggt ggttagagcg cacgcctgat aagcgtgagg
     1861 tcgctggttc gagtccagct aggcccacca tttgcccaga tagctcagtc ggtagagcag
     1921 gggactgaaa atccccgtgt cggtggttcg attccgcctc tgggcaccag gaatacggcg
     1981 gtatagctta gttggctaga gcgttcggtt catacccgaa aggtcatagg ttcgactcct
     2041 attaccgcta ccatctaact atatcaataa agaactttga aaattaaaca gtaggttaat
     2101 ttataaacaa gaaacaaacc ataaagccag atattttgat aacaatagta tctgagcctg
     2161 at
//
LOCUS       DACULQ010000303         2093 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.305, whole genome shotgun sequence.
ACCESSION   DACULQ010000303 DACULQ010000000
VERSION     DACULQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.305"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            26..1759
                     /locus_tag="JA154_18490"
     CDS             26..1759
                     /locus_tag="JA154_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT7997770.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENTQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
BASE COUNT          863 a          253 c          321 g          656 t
ORIGIN      
        1 atagcaaaaa gaaaggagga atatcatgag catactccta aaaaaagcac ccaaattagc
       61 aaaacatatt ataacaagtt tttatataaa tcgggatatt gatgaagtat taaaatacct
      121 ttgtgaaaat gttacatgga tcggaccagg agaacaggaa tttctcactt cttttaatga
      181 aataaaaaat tatttttatg caggtcaaga tgaaattcct tcttgtgata taaataatga
      241 tatatttgaa actgtaagtg aagatgagaa tagatgtatg gtattaggaa gatatactgt
      301 tcgaactaaa gaaaatactc aaatgatttt agaggtaaat caacgttgta catttgagat
      361 aattgaggat agaggaaaat tattagtaaa gcacatgcac atatcaaacc cttatggtga
      421 aatgcaactt gatgaatact ttcccactaa aattggaact caaagttatg attatttgca
      481 aagattacta aaggaaaaaa cagaagttat tgaaatgatt acaaataata tcaatggtgg
      541 cttaaaaggt agcaatgatg ataatacata ctcatttttc tatgtcaatg aaggattgcc
      601 caaaatatta ggatacacat atgatgagtt catggaaatg agtggtggtt cagcagtggg
      661 tgctgtatat ccaccagact tacctaaagc actagaagat tgtgaacgat gttttgcaaa
      721 aggacctaca tattcatctg aatatagaat ccgaaaaaaa gatggaacat taatgtgggt
      781 attagattct ggaatgaaat cattaaatag tgatggcata gtaaaaatta atagtattat
      841 tactgacata acccaactaa aaaacataga atctgaacta aaattagaaa gagagcgata
      901 tcgtattgct ttgcaaaata ttacagatat catgtttgag tatgatattg aaaacgatac
      961 ccttataaaa tatcaacgtg tagaaattga caaaaaaatt gaattagaaa attttgaaac
     1021 aaaaaattac tccaaactat tagaatctgg taaaattatt caccttgatg atattggaaa
     1081 actactagaa gtacttcatg gaaatcttcg tgaaacaatt gagataagac aagtaaattc
     1141 attaactaaa aatgaatgga gatggattcg tgttcaatgt tcagttatat atgatagtga
     1201 ccataaccct atcaaaacaa taggcgtttt aaaagacata actgaagata aatctaaatt
     1261 agaaagctta attaatcagg ctcaacgtga cccactaaca caattatata atcaaagagt
     1321 atcacaaaat cttattgaag agtatctatg tagttctgat tccaaaaata atgatgctct
     1381 attagttatt gatattgatg attttaaaac tgtaaatgat acttttgggc atttagaggg
     1441 aaatgaagtt ttagttgctg tatcaaaaat actattacat aacacacatg ataaagacat
     1501 agttgctcga attggtggag atgaatttac aatatttata aaatcattaa caaaggattt
     1561 aataataaaa attactaatg atattttaaa tgatgcaagt aaaataaaag taaaggacaa
     1621 tcataaaata acattaagta ttggtattgc cttcactgat gattcaacta aattatataa
     1681 agatttattt tctaaagctg ataaagctct ttatctatca aaagcagatg gcaaaaattg
     1741 ttatagtaca tacgaataat aaagctattt atacgaccaa gaatgaacct ttaaatttaa
     1801 tttcatagtc taacaatcaa agagttgaag ctaatcttat agctagtcaa ctagttccta
     1861 gaaaaaattt aaacaatata tttattgata ctgctcattt ttctaaagag caaatttata
     1921 taaaaaatta aacttttatg tacatacaga tactaatgat aaacagttat cattagtatc
     1981 ttctatctta aaacaaaatg ataatttatc tatataaaaa agtgctttgt tgacagagtt
     2041 cactttttta tataggtaca gaatttaagt ataaatacat gttttattct ata
//
LOCUS       DACULQ010000304         2069 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.306, whole genome shotgun sequence.
ACCESSION   DACULQ010000304 DACULQ010000000
VERSION     DACULQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.306"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            261..1172
                     /locus_tag="JA154_18495"
     CDS             261..1172
                     /locus_tag="JA154_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HAT7997771.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1347..1844
                     /locus_tag="JA154_18500"
     CDS             1347..1844
                     /locus_tag="JA154_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7997772.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
BASE COUNT          871 a          143 c          369 g          686 t
ORIGIN      
        1 ataacaagtg aggtaataat aagtgaattg atatagaaat aagtaaatta agtaagacga
       61 taacaaatga gatgataata agtaaattga tatagtaata aataaattaa gatgataata
      121 tccaaagtga gaaagataat agaaagtaaa taaaatcatg taagaataat ttagataaga
      181 ttctaagatt ttatttattt aaaaattagt atgattaatt tagattaaat aaatttaata
      241 attttagtag gtggtaatga atgaaaataa atagattaac agaaataata gtaattctat
      301 taaataaaaa attagtaaca gctaaagagt tggcagatag atttgaagtt tctacaagaa
      361 ccatatatag agatatagaa actctgtcta tgtcgggagt accagtatat atgacgaaag
      421 gcaaaggagg agggatatct ttaatagaag aatattctat agacaaggct atattgtcaa
      481 aaaaagataa agagagttta atagtggcac tgaaaacatt acaggcgact aagtatccag
      541 aaataaattc agtagtaaat aaaattggct caatatttgg agagcagaat ttttcaaact
      601 ggattgaaat agactttact gaatggggaa gtaattttaa tgaagatgat aaatttacaa
      661 aaataaaaga agctatttta aggcgtaata ctataaattt taattatgta aatagtttgt
      721 caagccagac aaacagaact gtcgaaccac tgaagcttat gtataagagt aagacttggt
      781 acttgtatgg attttgtaaa ctaaaagatg attttaggat atttagaatt agtaggataa
      841 ggaatttatc tataaaggat gaagtatttt caagaaagat aatagaagaa gtatgtttga
      901 atgattctaa agtaattaag gaaaatacta taacgttgaa attgaggttt aaagaaaaga
      961 tgctttttag agtatttgat gattttaata aggatttaat aactaaaaat gaagatgata
     1021 catatgatgt aattacagaa tttcctattg gtgaatggat ttatggatat attttatcat
     1081 ttggtgacaa tgttgaagta cttgaaccta aagatgttag ggataatgta ataactagac
     1141 taagggagtt gtctaaaatt tattcattat aatataatgc tagattaaag attttattaa
     1201 atatataata tcttgcattt agtatataat atctcactga gtttaattat attaaaaata
     1261 tgacataaag gtgtcgtata ttataaatta taattgaatt agttcaaaat aaaatatatt
     1321 aataaaaaca aagtgaggta taaagtatga attatgaaat agtagagata aatgagaaaa
     1381 tagttatagg aatatcgaag gagactacaa ataaagatgg acaagcggta aatgatatag
     1441 gtgaattgtg gaaaaaattt atgggcaaag ggatatataa tgctataaag ggtaaaaaga
     1501 atgacaagac aataggtcta tatacagatt accagggtga ttttacaagt ccgtatagct
     1561 ttgttgcatg ttgtgaagta aatagtaatt ctgataaaga gaagaattta gaagaattaa
     1621 acactaataa tactgttgga gaaagtatta ttagtaaagt tataccagca ggtaagtatg
     1681 caaagtttgt tatagtagga ggtcaaaaag aagtaggaga tttttggttt gagttttggc
     1741 aaatggattt tgataggact tatataagtg attttgaaga gtatcaatgt aatacttttg
     1801 atacaaagaa acaagaagtt catatttata taggaataaa gtagatttgt tattactata
     1861 ttgaatgaag tattgagtat agtacattta ttatagatag ttttattatt tataactata
     1921 agcagtaaat ctgtaaaaga tttactgctt ttttagtaat taatattatc agtatcactt
     1981 tgaatcttca gacatattct tcatttaata ttgtttattg ataaatgata cttgtaaata
     2041 taatgatttg ttatttaatt taaaatatg
//
LOCUS       DACULQ010000305         2065 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.307, whole genome shotgun sequence.
ACCESSION   DACULQ010000305 DACULQ010000000
VERSION     DACULQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.307"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..322)
                     /locus_tag="JA154_18505"
     CDS             complement(<1..322)
                     /locus_tag="JA154_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997773.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKI"
     gene            621..830
                     /locus_tag="JA154_18510"
     CDS             621..830
                     /locus_tag="JA154_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997774.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            850..1071
                     /locus_tag="JA154_18515"
     CDS             850..1071
                     /locus_tag="JA154_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997775.1"
                     /translation="MNILVCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKY
                     SSTCSILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1288..1686)
                     /locus_tag="JA154_18520"
     CDS             complement(1288..1686)
                     /locus_tag="JA154_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997776.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
BASE COUNT          783 a          225 c          272 g          785 t
ORIGIN      
        1 ctattttagc tttatcataa tcatttaatt ttctaaaatt gtttaaaagc tttatttcat
       61 tagagtttat gctattaagt ggatagtttg aggaggaatc gcaaattaaa tctgatttat
      121 gtgatagatt atctccattt aatagccagt ctactgaaac attaaatatc tcagctatag
      181 attttaatat ttcataattt ggttttctaa tgtttctctc aaatttactt aagttgtcac
      241 agcccaacat ttcttctagt tcatattgtt taaggttttt tgcttttctc aaataaacaa
      301 ttctttctcc taaagtatcc ataaacactc tccattcaat taatgtcaaa aagacttttt
      361 aagatgtaaa tagtttcaaa ttaaaggtca aaatgacata aaaaccattg acttaaggtc
      421 aaaatgactt tataattaac ttaatgatac gaatttacat cctaatttta gcacaaagta
      481 atcaaaaaat cttatttagt attaaataaa tttatatact taatatgtgt acatattaaa
      541 aatatatact aaatagaggg ggtgcgtaag ctaaaataat ataaaagtaa atataaatcg
      601 cttagaaagg aagttgataa atggatgctc gaaaaaaatg gatacctttt ttgggagtgc
      661 aagtcaagca aagacttatt gaattaaata tgactcaaag ggaattagcg aagaaaatag
      721 gtgttaatga aaactatttg tcagctattt taaatggaag aagaacaggt aaaaaatata
      781 aatcatcaat ttatcaatta cttaatatag aatattcaga agatgattaa taaatagtat
      841 ataaagtagg tgaatattct tgtgtgtaaa ttggattcag atggggttat agagtgttgt
      901 agagcaattg atgattttat tacagcactc agtaatataa aaagcttaaa tatggaaaga
      961 ttaaatactt taactaaata ttctagtaca tgttcaatcc ttcttaaaga ggggaattat
     1021 gaaggatgta caattgtgta tagaaagatg ttggaagaat taaaaacatg agtaatgcat
     1081 ttcttaggaa tataagttat acatagagat gtattatatt tttcaaagta cttaaactaa
     1141 aatatggata agataatcta aatattataa atgtgcttga aattagacta tacttgtttt
     1201 taaataatcc aatatccata ttttagtaat atactacaaa aaaagaaggt taatagatga
     1261 tgtaaaatcg tatcaaatta tgtatgttta aaccatttta tcttcattat tattagagga
     1321 atgctttttt aagtctttat attcagatat cttaagttca agtattcctt ctatttttat
     1381 tttatcacgt tcgtttagtt gtctgtatag atttaatatc gtcatttcat cattagtaac
     1441 atgtaagtaa tcttctttat cttcttttac actactattg acatttacct tctctttacc
     1501 atagagaagc cagtcagtcg taacattaaa ataatcagct attgacatta gtatatcaca
     1561 attaggtttt ctatctcctg tttcatactt gcctaagttt tcaaatttta aaatatccat
     1621 aagtttgcgc tgagtaagtt ttttggagtt tctcaaataa gcaattcttt ttcctaaagt
     1681 atccacaaaa tacactcctt tctttttatg agtaatgtct aaatgacatt taaaattaaa
     1741 aatatataaa tttataatat aaaactacta aattaaagtc taaatgacat tttgcttaaa
     1801 ttaatatgct cataatatga ttttaacata ttatagttga aaatatatgg tttattttga
     1861 tttgtatata taacaatagg tttaattgtt atataaatgt aaaggggtgt atgaatagat
     1921 tgtataaatt tatttcgata aactaagatt gctttttgat tgtctgtaaa agagaaaaag
     1981 attaagagaa aaatagtatt atattgtaat ttatattaat caattacaaa aattttatga
     2041 atttattctt tagaataaaa tattt
//
LOCUS       DACULQ010000306         2054 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.308, whole genome shotgun sequence.
ACCESSION   DACULQ010000306 DACULQ010000000
VERSION     DACULQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.308"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            58..>2054
                     /locus_tag="JA154_18525"
     CDS             58..>2054
                     /locus_tag="JA154_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT7997777.1"
                     /translation="MDKKKLILITHDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKG"
BASE COUNT          867 a          247 c          296 g          644 t
ORIGIN      
        1 cttgccttat actttaaata ctttaaaaat aaatactatt ttgttaggag aaataaaatg
       61 gataaaaaga agttaatcct tataacacat gataaattga atagtgacca ctacaaagaa
      121 gaattaacaa acttttttgg agacgaaata atcatagaaa cacaaaatat acttgatgga
      181 ataaaagaaa atttagaggg agatgtagtg ctttctctat cacctttaac tagtaatttt
      241 ttaataaaac attttaagga agatattgaa attattcatg gaacgaaagc actttcaaaa
      301 cttggatatg aaaaaatgat gaaactacct ccaggtacaa aatcgttact tatgacaacc
      361 aataaaactt cagcatttga gatggctact tatctatata aaattggtat aaatcatata
      421 gattttgtac cgacatatcc agattgtgat gaaatatatg atttagatac agctataaca
      481 ccaggtcaga ttagatttat accaaaatac ataaaaaata ttgttgattt aggttggaga
      541 aaaatctcat tagatacata tatgtcatta ttagtagtat taaaactaaa aaatgagaaa
      601 tttatagaaa aactatacaa attgtccaaa gagacactaa gtcatgattt cttaaataca
      661 tcattagata atatatctaa attaaaaact atactttaca tgaccattga tgaaataggt
      721 gatggtctaa tatttttcaa cacctttaac aaggttactt ttgtaaataa gtctttactt
      781 aatatgttgg gactagatga aaaacttata aaatcacctt ccttaatgga atatatgcca
      841 aatagtttct tagataaaat tacaaagaac ttaaatatcg ataatatgat tatatacata
      901 gacgaaatag ataaaaaatt catactttct aaaaaaccat tttatcttta taaaaatata
      961 gagggttgct taattacatt gaaagatgta aacaatatag aaatattaga acaaaaaata
     1021 agaagtgatt ctgtcaaaag aggttatgta gctaaatata aatttaataa tattattgga
     1081 aatagttcta ttataaaaga ttgtattaaa aaggcaaaaa aaatggctct tacagataat
     1141 cctatattaa taacaggtga aacaggaaca ggtaaagaag cctttactca gtcaatacac
     1201 aatcattcaa atagaaaaaa taaacctttt gtagcaataa attgtgcttc attaccaagt
     1261 gaattacttg aaagtgaact atttggatat gaagatggtt cttttactgg agcaaaaaaa
     1321 ggtggaaaaa aaggattatt cgaattagct cacactggca ctatattttt agatgagata
     1381 ggagatatgc cacatgattt acaggttaaa cttttaagag tcttacaaga aaaagaaatt
     1441 agaaaaattg gtggtacaag tattattcca attgatgtga gaatattagc tgccacaaat
     1501 aaagatttag agaaattaat tatagaaaat aaatttagga tggatttatt ttataggata
     1561 agtatgttca ctttagattt acctcctctt agaaagagat tagaagatat acctcttctt
     1621 ttagaatcat ttttaaaaga acttccctat aaaaacataa aactcgataa gagtctttta
     1681 gaagctttaa attcatacac ttggatgggg aatattagag aactgagaaa ttgtattgaa
     1741 tatatggctt acatgggttc caattatctt acaataaatg atttacctca aaacatatct
     1801 tcaaaattaa ataataatca tatgtcaagt aatatgtcca tattcaatga tttaaaccaa
     1861 tatgacaaaa acatatgcat ttctatccta aaatcattgc acatgaaacc aatggggagg
     1921 acaaagttaa tgaaatttat ggaatataat gtcacagaat acgaagtaag aaatatgcta
     1981 gaatatttga cacgcaatgg atacttaatt tctagtaaag gaagaaaagg ctcatcaata
     2041 actgagaaag gcat
//
LOCUS       DACULQ010000307         2026 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.309, whole genome shotgun sequence.
ACCESSION   DACULQ010000307 DACULQ010000000
VERSION     DACULQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.309"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            73..873
                     /locus_tag="JA154_18530"
     CDS             73..873
                     /locus_tag="JA154_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT7997778.1"
                     /translation="MELERPLLYDEVENKDDKNNNRKFNIKNKIFKGIGILFVLILLG
                     FFIWINKIYEPQKLAKEALVSNSKVEVTVNENISFTPKGRKVSKGLILYPGAKVEIES
                     YAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSYNNIEHWVIGGHSLGGVAAS
                     NFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKDGVVNFKSLVESKQKLPDDT
                     TYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANSIIKFLKNIS"
     gene            982..1839
                     /locus_tag="JA154_18535"
     CDS             982..1839
                     /locus_tag="JA154_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT7997779.1"
                     /translation="MPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIKPLEV
                     IKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYGSPKS
                     IEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKSGIKL
                     SATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGEFTIL
                     EDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYLSNQK
                     LKREEYRRL"
BASE COUNT          841 a          200 c          365 g          620 t
ORIGIN      
        1 atgggggtta gatttatgag taaagatgta aaggtaaagc gtaataagaa atatgataca
       61 tttagtataa ctatggaact tgagagacct ttactttatg atgaggttga gaacaaagat
      121 gataaaaata ataatagaaa atttaacata aagaataaga tatttaaggg aataggtata
      181 ctatttgtat tgattttgct tggctttttc atatggataa ataaaatata tgaacctcaa
      241 aagctggcaa aagaagctct agtcagcaat agtaaagttg aagtaactgt aaatgaaaac
      301 atttcattta caccaaaagg aagaaaagtt tctaaaggtt taattttgta tccaggagct
      361 aaagtagaaa tagaatctta tgctccactt gcaagaaaaa tagcagaaag tggatatgag
      421 gttgtaatcg taaagatgcc acttaatcta gctatacttg gaactaataa ggcacagaaa
      481 gttatggatt cttacaacaa catagaacat tgggtaatag gaggtcattc attaggagga
      541 gttgcagcat caaattttgc acgtgataat aaattaattg aaggcgttgt atttttagct
      601 tcttatccaa tgggtgatga gttgaaagaa ttaggaaaaa aagttatatc tatatggggg
      661 agcaaagatg gagtagtcaa ttttaaaagt cttgtagaat caaaacaaaa acttcctgat
      721 gatacaactt atgttgagat tgaaggagga aatcatgcac aatttggaga ttatggaaaa
      781 caaaagggag ataatgatgc tattataagt caagaaaaac aacttaatat tactgccaat
      841 agtatcatta aatttttaaa gaatatatct taaactaaaa tttaaaaaat ataattttat
      901 aagttattat ttaaagtaaa tattaataat ataatagaat gaatatttta ttacaagggg
      961 gattttaata tggaatacga tatgccactt tacagaccac caagtgaggc ttatagtctt
     1021 ataatacaag tcacactagg atgttctcat aacaaatgta cgttttgtag tatgtacaaa
     1081 tctaaaaaat ttaaaataaa gccactagaa gtaataaaaa atgaaattga tatatttaga
     1141 agatattata agcatgtaga aagaatattc ttagcagatg gagatgcatt gataatacca
     1201 atggagaaat taagagatat tatcttatat ataaaagaag tttttccaga gtgtgaaaga
     1261 attactatgt acggctctcc taaatctata gagaaaaaat ctgatgatga acttaaagaa
     1321 ttaagaaaac ttggtgttaa aatgatttat ctagggcttg aaagtggaag caatgaagta
     1381 ttagaagata ttaagaaagg ttttagtagt gaacaattaa taaaagttgg tagaaaagtt
     1441 aaaaaaagtg ggattaaatt atcagccact gtaatagcag gtcttggagg aactaaaaaa
     1501 acacatcagc atgcagttga tacaggaaaa atgttaggag caatttcacc agattatgtg
     1561 ggtgtactta gtttaatggt agaacctaat actgaacttt acgatttact taaggcagga
     1621 gagtttacaa tactagagga taaggctgtg ttacaagaaa ttaaagagat gataaagaat
     1681 atagatgcta atgaaaaaat tgtatttaga agtaatcatg catcaaatta tgctaattta
     1741 aaaggtgtat tacctgatga taaacaacgt ctaattgatg aaattgatta ctatctaagt
     1801 aaccaaaagc taaaaagaga agaatataga agactatagt ttttgatata tcaatctata
     1861 ttgataggta aatactaaga ttatttagat acaaaaaatc atgctacatt aatagttgct
     1921 tactattgat gtagcatgat ttttttatta aaaaacaaaa tggtatattc atatataata
     1981 tatcaataat aatgaaaatg tgaactagtt taatagaatt ttacct
//
LOCUS       DACULQ010000308         2003 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.310, whole genome shotgun sequence.
ACCESSION   DACULQ010000308 DACULQ010000000
VERSION     DACULQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.310"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>2003
                     /locus_tag="JA154_18540"
     rRNA            <1..>2003
                     /locus_tag="JA154_18540"
                     /product="23S ribosomal RNA"
BASE COUNT          542 a          427 c          601 g          433 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tactaccgat aaccgtttga gagaagggat
      781 gacacagtag gataagctaa gcacactgtt ggttatgtgt gcccaagcat tgaggcagtc
      841 aaagtaggca aatccgcttt gataatgctg ggatgtgatg gggagcgaaa tttagtagcg
      901 aagtagctga tttcacactg tcaagaaaag tctctatcga ggttaaaggt acccgtaccg
      961 caaaccgaca caggtgggtg aggagagtat cctaaggcca gcgagagaac tgttgttaag
     1021 gaactcggca aaatgacccc gtaacttagg gataaggggt gccaccatca ggtggccgca
     1081 gagaataggc ccaagcgact gtttaccaaa aacataggtt tctgctaagt cgcaagacga
     1141 tgtataggag ctgacgcctg cccggtgctg gaaggttaag gggatctgtt agagcaatcg
     1201 aagcagtgaa cttaagcccc agtaaacggc ggccgtaact ataacggtcc taaggtagcg
     1261 aaattccttg tcgggtaagt tccgacccgc acgaaaggcg taacgatttg ggcactgtct
     1321 caacaacaga ctcggtgaaa ttgtaattcc ggtgaagatg ccggatacct gcgacaggac
     1381 ggaaagaccc catggagctt tactgtagct tgacattggg tcttggtact acatgtacag
     1441 gataggtggg aggctttgaa accaggacgc cagttttggc ggagccatcc ttgggatacc
     1501 acccttgtag tactgggact ctaaccatag gccatgaatc tggtcttggg acactgtcag
     1561 gtgggcagtt tgactggggc ggtcgcctcc caaaaggtaa cggaggcgct caaaggttct
     1621 ctcagtacgg tcggaaatcg tacgtagagt gtaaaggcaa aagagagctt gattgcaaga
     1681 catacaggtc gagcaaggat gaaaatcgga cttagtgatc cggtggttct gcgtggaagg
     1741 gccatcgctc aacggataaa agctaccctg gggataacag gcttatctcc cccaagagtc
     1801 cacatcgacg gggaggtttg gcacctcgat gtcggctcat cacatcctgg ggctgtagta
     1861 ggtcccaagg gttgggctgt tcgcccatta aagtggtacg cgagctgggt tcagaacgtc
     1921 gtgagacagt tcggtcccta tccgtcgcag gcgtaggaaa tttgagaaga cctgtcctta
     1981 gtacgagagg accgggatgg acg
//
LOCUS       DACULQ010000309         1994 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.311, whole genome shotgun sequence.
ACCESSION   DACULQ010000309 DACULQ010000000
VERSION     DACULQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.311"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(98..472)
                     /locus_tag="JA154_18545"
     CDS             complement(98..472)
                     /locus_tag="JA154_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT7997780.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            691..924
                     /locus_tag="JA154_18550"
     CDS             691..924
                     /locus_tag="JA154_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997781.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            908..1321
                     /locus_tag="JA154_18555"
     CDS             908..1321
                     /locus_tag="JA154_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997782.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            1340..1636
                     /locus_tag="JA154_18560"
     CDS             1340..1636
                     /locus_tag="JA154_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997783.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITNEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
BASE COUNT          691 a          254 c          273 g          776 t
ORIGIN      
        1 tttcttaaca tgttcagaat aataaacaaa atgaaggtag aaaaagttat aatttcatac
       61 cttcatttta tttattatgt atcaatttgt cattttctta tttcaaattc ttaaaatatt
      121 cttcgagttc acttaattta tcttcatcca cttcgtcgtc ttcaccatgt aacgctgaca
      181 ttaaacttga aattgaattt ttatgtatgt tcttcaaaaa ttcctttgtt tcaaacctta
      241 gatatttttt ctcttttact agtatattat aatgtgtaag tttgcctatt ttctctgtac
      301 ttaaaaactc ccttcgctct aatcttttca aaactgtaaa tgtagttgat tcattccact
      361 catatttttc ttccattgca gcaactaatt ccctagaagt taataccttg ttagcttcac
      421 aaatatatct cataactttt aattccgctt ctggtaattt actaattttc ataattcgcg
      481 cccctttgcc gatttatgta tatgttaaat tctatatcaa taaagtttta caaacaagag
      541 aattaaggta aataattcca tccaattttt accatattat tcttttaggt tttataatac
      601 ttatctaatg agattgacat tttactcatt ttaggatatt atgaagctaa cttcacatga
      661 agttagcttc atatagaaag aggttatgga atgaaaacaa aagtacgaga atttcgaaca
      721 aatatgggtc taacacagca acaactcgct gatttagtcc atgtatcaaa cagaacaatt
      781 atttctattg aaaaagagca atatagccct tctcttatgc ttgcctatcg tatggcacaa
      841 gtgtttggta caacgattga agaactatgt tgtttaaaag aaaataaaga aatggaggat
      901 aaaaaacatg aaagtaaaga gtaaacgaag ttttatagtg ggtattatag tttgtatgtt
      961 atgttgtgct agtttagtta tttattgcat tttaaaagat aaaagattcc taatttcatc
     1021 atttctttta attgtaatag caatctttaa tttttgtaat gctttttcaa gaaaaggcat
     1081 tgttgaagaa ttacacgata gtgcagatga aagagacttg tacttaacaa tgaaaacaag
     1141 tcatatctta gttaaaatta tgaattatac attatttact tttacatttc ttttcatcat
     1201 agcttatagt gcatggaaaa atcaatcact tctagttata gctataacac tatgtgtcat
     1261 tgagattttt ttatttgttg catatttact tatcaatata tttttagaga aaaaagagta
     1321 aatacaaggg gtgatataaa tgtattttaa aacttatata ctaaaaagag aagtaaataa
     1381 caaacaacta gagataactc gaagttttga cttagataat ctaatttgta ctgcaacaga
     1441 gattttgaaa gataatgaga ttgatttgat aattactaat gaagtaaata atgttgtttg
     1501 gagaaatgaa aaaagcactc ccccagcaag agaatgctta gttatagcta tagaagtgta
     1561 ttggtggtac acttctattt ttttattata tcatataaat tatgttatca aaaagagcta
     1621 tttaaatcga ttttaaggtg tgttgaataa ggttcttgat agtttatata ttgatgaatt
     1681 tcaaaaaaat aagcactctt tattgtaagg agtgctttgg tgtatgttaa tcttctatct
     1741 aatacaatta taacatgtct tatgttttag tatgataatt ttcgttcgct ttattattac
     1801 aatttttact atagttttat ctatcctgtt aatatttgta atattttaga tgctctttgt
     1861 acttgtgtta ctgatatact ttgattaaga aaagcaaatt taataggagg tatctgctat
     1921 ggattttgga aatatcaatc ttatacttat tggaataata gttataatag gaactacaat
     1981 aatatattta atta
//
LOCUS       DACULQ010000310         1975 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.312, whole genome shotgun sequence.
ACCESSION   DACULQ010000310 DACULQ010000000
VERSION     DACULQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.312"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            172..1791
                     /locus_tag="JA154_18565"
     CDS             172..1791
                     /locus_tag="JA154_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent phosphate transporter"
                     /protein_id="HAT7997784.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDEHFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
BASE COUNT          729 a          244 c          307 g          695 t
ORIGIN      
        1 aaaattaaca acaacttaaa aatttaacat tctcttaacg tatacttaac aaaacattaa
       61 tatttttatt gacaaaattt gttattctat attatggaaa caataaattt tagttaataa
      121 agtttataat tcagataaaa aaattattaa tttcttaagg gaggaaatat cgtgaatata
      181 gtaattagcc ttatcggtgg acttggactt tttctttacg gaatgagttt aatgggagaa
      241 ggtttacaaa aatcagcagg agacaaatta aaaaaaatta ttgaactctt aacaagtaat
      301 gttgttatgg gtgtactggt aggtacagtc gttacaggta ttatacaaag ttcaagtgct
      361 actactgtta tggttgtagg atttgtaaat gcaggtataa tgaatctaag tcaagctatt
      421 ggtgtaataa tgggggcaaa tataggtact acagttactg ctcaattagt ttcatttaat
      481 cttgaaggaa ttgcaccaat tgctttaggt ataggtattt tattctattt atttacatca
      541 aatcaaaaaa caaagcattt atctgagata cttataggtt ttggtatatt atttacagga
      601 atggagttta tgaaagatgc agtagctcca cttgctgaat acaaagcatt cactgatgca
      661 cttttatatt ttagtaaaaa tcctgtatta ggtatattgg caggatttgc cataactggt
      721 attgttcaaa gttcaagtgc atctatgggt atgttaatag cactagcttc tcaaggaata
      781 cttcctctat catctgcttt acctattctt tatggagata atataggtac ttgtgttact
      841 tcacttttat caagtgttgg tgcaagtaga aatgctcgac gtgcagctgt aatgcattta
      901 agttttaatg taattggtac aataatattt atgcttgttt taaataaacc tatatcagcc
      961 atagttactc attttgaccc aactgataca gctagacaaa tagcaaatgc acatacttta
     1021 tttaatctta caaatgttat aatattatta ccattttcta aatacatagt taaattagca
     1081 aatagactta tacctattaa agaaactgaa tcagaaatag taaataatac taagtactta
     1141 gatgaaagga tgtttagcac tccatctata gcacttggta atactgtaca agaagttgta
     1201 agaatgggtc ataaagctac aaactctttg gaacattcaa ttgcaggatt tttaaataag
     1261 agcaatgaag atattaataa agcttttgag tcagaaaaag ttgtcaataa gttacagaaa
     1321 gatatattga actatttgtt aaaactttct aaagaacctc ttagagatga tgaacacttt
     1381 agaacagatt tgctttttaa tactgtaaat gacattgaaa gagtctctga ccatgcagaa
     1441 aatatagctg aattggccat gagtgttaag gaaatgaaca ttagtttttc tgatagtgcg
     1501 ataagagaaa tatacgaaat atacaataag acaattacta attttaagga tgccttaata
     1561 gttttagatg ttaaagattt tgagttagca aataaagttt tagaagttga aaatgaagta
     1621 aattatttag aaaaaacttt tagaaattca catatgatta gactaaataa tggaagttgt
     1681 acaattgatg ctggtgtact ttatcttgac ttacttacta atctagaaag aatttctgac
     1741 cactcaacaa atatagtaaa acaagtacta aaacttaaac aaaaaatata gtgattattt
     1801 ttatatataa tcttaatcaa tgagaagtaa gaagctatag ataatgctta tatcatttat
     1861 ggtttcttat tttttatgta atactcaaat ttttatagta ttctcttatt attaaacaat
     1921 ttctgcctag aatattccat atatatatga cttctttcat aaaacttgaa ctgcc
//
LOCUS       DACULQ010000311         1941 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.313, whole genome shotgun sequence.
ACCESSION   DACULQ010000311 DACULQ010000000
VERSION     DACULQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.313"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            532..1572
                     /locus_tag="JA154_18570"
     CDS             532..1572
                     /locus_tag="JA154_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT7997785.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT          597 a          257 c          284 g          803 t
ORIGIN      
        1 tagttccttt tacatgaatc tttgcatcaa ctttttcatt ttttaacacc aacgtatcta
       61 catttttgct aattatcatt actaatatat taaatactaa atatataaga ataccaagta
      121 tcactaatct ggtatatttt acctttttca aagactctcc ccctataaaa ttactttcat
      181 tataagtact tcttctaaaa aatgtttatt ccttttttat aagctaatat tttacgttct
      241 aataattatt ttttccatat taaatttatt ttttatcaat cttcctatac aagactgcat
      301 tatttatata taattgtatt ataaagtata agtttaattt acattttata cgttaagctt
      361 actttatatg tttttatagt tatttttatt tttagatgca atataaaacc aattaagatt
      421 taaaataact tttgttatgt tgacatttat ttaggtaatc atttatgaat taggataacg
      481 ttatcataac aaaagtttat gtcctaaaaa aaatacaagg aggattttct tatgtcagaa
      541 attttattag gaacaggttt acttttattg gttctatgtt gtttcacaat ttttagttac
      601 aaggccccat atggtatgaa ggctatggga gctctagcaa acgctgcatg tgctactttc
      661 ttagtagagg cattcaacct ttctttactt ggtgatgttt taggtatgga attttttaga
      721 agtgttggag ctgctaatgg tagtcttggt ggagttgctg ctgctatact tgtgccttta
      781 gccttaggtg tatctccagt ttatgcagtt ttaataggat tatcttgttt agatttttct
      841 atattatctg gattcgttgc tggatacttt gtttctttct taattaagtt tatagagaaa
      901 aaagtgccac ctggagtgga tttagtggtt gtaattcttc tatgtgcacc gctatctcgt
      961 cttatagcat caaatgtaga cccattagtt aatagtacac tactacaaat tggacaagtt
     1021 ttgatagaag ctaccaacat tagccctata ataatgggaa ttatactagg aggtctaata
     1081 acagtagttg gaacgtctcc acttagttca atggctctta catctataat tggtcttact
     1141 ggtcttccta tggcaatagg agcattaggt gcttttggtt cagcttttat gaactcaaca
     1201 ctatttgcaa aattgaaatt aggttctaaa aaagatatta tatctgttgc tattgaacca
     1261 ttaacccaat cagacataat ttcagctaat ccaataccta tatattctac taatttcttt
     1321 ggaggagcct tttctggtat cattgtagcc ttgatgggtc ttgtaaacat gacaccaggt
     1381 acagctacac ctgtggctgg ttttgctata atgtttgctt ataatccagt tcaacaagtt
     1441 cttataacag ctggtttatg tgctttattc aatgtaattg caggattttt aggttcaaaa
     1501 atattcagta cctttaaagt tcttactgct ggtgaaatac gtggtactga tgtagaagaa
     1561 gaaattgctt aaaaaacaat aagatttata aatttatatc tcaagtagta gagttttata
     1621 ttgtaggcaa ttttattaat tgcaaacgtt ttatttttta tttatgttta tgattattat
     1681 gtgtgttgaa tatttagata aatttttatt tattattttt atttagattt ttgtttagtt
     1741 ttttatttgg ttgatatatt aggaataaaa attagtatag tttattacta gttagatagc
     1801 ataacttttt agatacataa aaaattttat attaattaga tattattaaa ataaaagcga
     1861 tacatgaatt tttaaatgtc catgtatcgc tttatttttg ccataatctt aacaactgat
     1921 ttttaaagtt tattatctta a
//
LOCUS       DACULQ010000312         1858 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.314, whole genome shotgun sequence.
ACCESSION   DACULQ010000312 DACULQ010000000
VERSION     DACULQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.314"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            200..469
                     /locus_tag="JA154_18575"
     CDS             200..469
                     /locus_tag="JA154_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HAT7997786.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            495..1850
                     /locus_tag="JA154_18580"
     CDS             495..1850
                     /locus_tag="JA154_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HAT7997787.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
BASE COUNT          699 a          195 c          407 g          557 t
ORIGIN      
        1 atataatata tgaattttct aaatagtata aaaataatag aatattgaaa aaaaattgac
       61 aaatgaatgt ataggtatta aaatatatta aatatataca taaaaagtgg tttactaaca
      121 aataaatgaa atatatgtta acacatagtt caatttgtaa aaatataaga tttataaaaa
      181 aataagggag gatattatta tgaaagcaat tttaacagta attggaaagg ataaaccagg
      241 tatagtggca ggaattagtg atgaattata caagcaaaat atcaacatag tagatatatc
      301 acaaaaaata ttacaggatt attttactat gattatgata gttgatttag agaaatcatt
      361 aaattctttt gagaaaacgg ttgaggactt ggttgaaaga ggtaagaaat tatcagtgga
      421 tgtaaaaatt cagcgagaag aaatatttaa ttcaatgcat aatctgtagt agacagtaag
      481 gggaggtgat aatagtgaat gtaggaaata tacttgaaac aataaagatg atagatgaag
      541 aacagttgga tataagaact ataactatgg ggatttcttt attagattgt atagaccctg
      601 atggagataa agctagagaa aaaatataca ataagattat gagttctgct aaagatttgg
      661 tgaaggttgg aaaagatata gagcgtgaat ttggtatccc aattgtaaat aaaagggtat
      721 cagtaactcc aatttccatt attgcaggtg caacagatga aaatgactat gtaaagtttg
      781 cacagacatt ggataaggct gcagaagatt taggtataga ttttattggt ggattctcag
      841 cattagttca aaagggatat acaaaaggtg ataagatact aattaagtca attccaaaag
      901 ctctagcaag tactaataaa gtatgtgctt cagtaaatgt aggttctaca agatgtggta
      961 taaatatgga tgcagtgaaa gaaatgggag aaatcataaa agaaactgca gaattaacaa
     1021 aagatgcaaa aggttttggt tgtgcaaaat tagtagtatt ttgtaatgca gtagaagata
     1081 atccatttat ggcaggagct ttccatggtg ttggtgaggc tgatagaatt ataaatgttg
     1141 gtgtaagtgg ccctggtgtt gttaagagag ctttagagaa agtaaaggga gagccttttg
     1201 atgttgtttc agaaacagtt aaaaagactg catttaaaat tacaagagtt ggacaacttg
     1261 tggcaaagga agcttctagt agattggatg ttccatttgg gataatagac ttatcactag
     1321 ctcctacacc tgctattggt gattcggtag caaatatatt agaggaaatg ggtcttgaag
     1381 ttgttggtac tcatgggact acagcagcat tagcactttt aaatgatgct gttaaaaaag
     1441 gtggagtaat ggcatgttca catgttggag gacttagtgg agcgtttata ccagtatctg
     1501 aagatgcagg tatgattgat gctgttatta aaggtgcttt aagtatagat aagcttgaag
     1561 caatgactgc tatttgttct gtaggtcttg atatgatagc tgtgccagga aatactacag
     1621 caggtacgct tggagccatg atagctgatg aggcagcaat aggtatgatt aataataaga
     1681 ctacagccgt tagaattata ccagccccag gatgtgatgt aggtgatatg gtagaatttg
     1741 gaggactttt aggacgagca ccagtaatgc ctataaatac aaattcatct gaattattta
     1801 ctcaaagagg aggaaggatt ccagctccta tacattcatt taaaaattag ttatatta
//
LOCUS       DACULQ010000313         1839 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.315, whole genome shotgun sequence.
ACCESSION   DACULQ010000313 DACULQ010000000
VERSION     DACULQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.315"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            356..1648
                     /locus_tag="JA154_18585"
     CDS             356..1648
                     /locus_tag="JA154_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAT7997788.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIISPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          686 a          219 c          311 g          623 t
ORIGIN      
        1 tatgaatgta aaaaaggaga gtgatatata cagtatataa agcaatacac ttaataacaa
       61 atcgaataat ctaaaaaatc ttaatatttt aattattcag aataattaat tttatcaaga
      121 aaaattagag aaaaaacaaa aaaccaactg tgtttaaagt atacaggttg attttgttta
      181 attaagaaag ttgtattaaa tattgtgaag attttataaa ttatgaaaca taattgacaa
      241 atttaagtca aaaatgtata attaaataaa atctcgtcaa aaggttttca tttaatgaag
      301 agattggact tattttttag taaaaggtag taaaattcaa atgggggtgc tttaaatggg
      361 tgagaaacaa atcaatttta aagatgtaat tgtaattgga tttgcactat ttgcaatgtt
      421 ttttggtgca gggaacttaa tcttccctcc atatctagga gttttatctg gaagtagttg
      481 gctaatagct ttcataggat tcctttttgc agatggtggt ttggcattat tagcagttat
      541 agcagcaact aagttcaatg gagacacatc gaagatgttc tcaagagcag gcaaaggatt
      601 aagtattgca ttaggatgtg caatggtaat ctgtataggg ccacttttag caatacctag
      661 aacagcagca acaacttatg aaatgggtat attacctaca ataggttctg gaataagccc
      721 agtaatattc tctatagtat tctttgctct agtattggtt ttaacaataa gaccatcaaa
      781 agttgtagat atagttgggt caatattaac tccagcttta cttatagctt tagcagtatt
      841 aatagttaaa ggtatcatat ctccattagg agaaataaga gacagttctt taatacaaaa
      901 tgtatttgca gaaggtataa ctcaaggtta tcaaacaatg gatgcattgg cagctagtgt
      961 atttgcatca attataatca tgtcagtaat agctaaaggt tatacaggtg agaaagaaaa
     1021 gatgaaagcc acaataagtg caggtgtaat tgcagtaata ggaatggcat tagtttatgg
     1081 tggattatgt tatcttggag caactgtgtc tcaacaatat ggacaagatg ttcaacaaac
     1141 agctttaata gttgcaataa cagcagcttt acttggaact acaggtaaaa ttttattggc
     1201 tattatagtt gcattagcat gtttaacaac agctataggt ctttcttcag cagctggtca
     1261 atatttctca acacttacag atggaaaatt aaagtatgag catatagtta tagtagtttg
     1321 tgtatttagt gcaatcatat caaactttgg agtaagtaca ataatacaat tctcttctcc
     1381 tatactaagt atggtatatc cagcaactat aactctagtt atactagcat tatttagtaa
     1441 taaaattaaa aatgacaacg tgttcaaatg tgctgcatac atggcattat tagtaagtgt
     1501 attaacagtt gcaacaagtt ttggtgtaaa aattcctcta gttaatagct taccatttgc
     1561 ttcattagga tttaactgga tagttccagt tgtaatagca ggaataatag gaaactttat
     1621 tccatctaaa agcagtaaaa ctgcataata ataaaaacta attgataatt atggaattgc
     1681 ctttaaatta tataggcaat tcctttttat atttaaaaga attattaaat tattgtagat
     1741 aaaagaacat tattaatcct atttgataaa tttaaaaatt tatttttgga gtagtatgat
     1801 ttataaagat aataaaataa aaaagattca tttataatt
//
LOCUS       DACULQ010000314         1822 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.316, whole genome shotgun sequence.
ACCESSION   DACULQ010000314 DACULQ010000000
VERSION     DACULQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.316"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            99..1091
                     /locus_tag="JA154_18590"
     CDS             99..1091
                     /locus_tag="JA154_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAT7997789.1"
                     /translation="MYLFYGREYYLIENAIKAFKDSLNEGMLDFNLDIIDGKEIVLNQ
                     LISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLIEHLDNIPDTTTIVFVVY
                     GDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALNDVIIDNSQIMYFIEQEG
                     YRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQKKVENDIFKLIDYIGEQN
                     ASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLDGYSTKLIADKLKMHQFV
                     VGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAVEMLVSRYCKREAI"
     gene            complement(1278..1544)
                     /locus_tag="JA154_18595"
     CDS             complement(1278..1544)
                     /locus_tag="JA154_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAT7997790.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT          716 a          178 c          273 g          655 t
ORIGIN      
        1 tttttacata ttttacatag aaggaaaaga gataaaggag caagagaatg aattacaagg
       61 atattataag gaatgtaaaa gagaaaaact ttgaaaaaat gtatcttttt tatggaagag
      121 agtattattt aattgagaat gcaataaaag catttaaaga tagtctaaac gagggaatgc
      181 ttgattttaa tttagatata atagatggga aggaaatagt cttaaatcag cttataagct
      241 ctatagaaac acttccattt atggatgata ggaagatagt aataataaaa gactttgaac
      301 tattaaaagg aaagaaaaag aattttacag atagtgatga gaaatactta atagagcatt
      361 tagacaatat tccagacacc acaactatag tttttgttgt atatggagat gtagacaaaa
      421 gaaaatcatt agttaagaaa ataggaaaca atggaattgt ttttgattgt gataagcttt
      481 ctgatatgga tttgtttaaa tggataaaaa aatcttttgc tttaaatgat gttataatag
      541 ataattctca aatcatgtat tttattgaac aagaaggata cagagataag agtagtgaaa
      601 agacattatc tgatttagaa aatgaaataa ataaaataag ttcatttgtt ggtaaaggga
      661 ataatgtaac caatgatgta attgataaat tatctcaaaa aaaagttgag aatgatatat
      721 tcaaattaat cgattatatt ggagaacaaa atgcttctaa tgcaatgaaa atattaaatg
      781 atatgataca agaaggcgaa tctgtattag gtatattttc tatgatagca agacaattta
      841 aaataattat gcaagtaaga cagttacaac ttgatggata ttcaacaaaa cttatagctg
      901 ataaattaaa aatgcatcaa tttgttgtag gaaaagcatt aaagcaaact aaaaattttt
      961 ctgatgatat aattgtggaa atattaaact atatattaga aagtgattac aaaataaaga
     1021 caggtcttat aagagatact ttagcagttg aaatgttggt tagtagatac tgtaaaagag
     1081 aagcaattta aaaaatctat atattaataa aaaataaata gcaagatatg ttatagttca
     1141 gagtagatgt aagctaaatt aagtttgata tacaatcaac tataatatgt ctaactattt
     1201 attttttatt gtataaattt acaaaataaa aaacccctac ataggggtta ttatatataa
     1261 gaaatataaa ctggtaatta agcgttcata ccgtttaatt tttgagctaa tttagctact
     1321 tttctagctg cagcattctt atgtatagtt cctttagaag ctacttggta tattcttttt
     1381 tgagcgtatt ggaacttagc aacagcatcc tctctgtttc cagctgcaac agcatcttcg
     1441 aatcttctta ttgcagtttt tatttgagat tttctaactc tatttaaagc agttttcttt
     1501 tcgataacac ttattctttt cttagctgat tttatatttg ccaccgtgtt cacctccctg
     1561 tattttttta tcatattcat cgtcaacata ttagatttta acagaaataa atctaaaaat
     1621 caaggattat tatttatata ttttaataat gttttttatt ttgatttttt agactgtaaa
     1681 ttgtgtataa ataatttgca atatcttgtt tttaaattgg tatattttgt gttttaaata
     1741 ttttgacata tataattaat atttcatttt gtaacaaata tttgagataa tacaaactat
     1801 taacatacat tttgatagta tt
//
LOCUS       DACULQ010000315         1808 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.317, whole genome shotgun sequence.
ACCESSION   DACULQ010000315 DACULQ010000000
VERSION     DACULQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.317"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            62..409
                     /locus_tag="JA154_18600"
     CDS             62..409
                     /locus_tag="JA154_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAT7997791.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(559..1608)
                     /locus_tag="JA154_18605"
     CDS             complement(559..1608)
                     /locus_tag="JA154_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT7997792.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
BASE COUNT          615 a          278 c          243 g          672 t
ORIGIN      
        1 tattttctta tattggtata ataatagtaa atctataata tagaaaagag gtatttctag
       61 tatgcatgaa aataaaaaaa atctattaga cggaagctta gctcaatata atgatacagc
      121 agttccaaaa ataccagtat ttgctggtaa cgatataact tcagaagttt attattttaa
      181 acctaatcaa gttcttaatg ctcatagaca ccctaatggt gaacaaatat ttgtgttcct
      241 aaaaggagaa ggtaaaatga aacttggtga gcatgaatgt gatgtaaaaa acggagatac
      301 tgtatttgta ccaacaggag agtggcatga aattactaat ggaagcaatg aagaaatggt
      361 agccgttcaa attacaaaaa ttaatgctgg agcagaatac agaggataga tatataagta
      421 cagttagtac tttaatatct attttattat ataaacttta atctcaaata taaaaagagt
      481 cttttaaaag gctcttttta tatttttgtt aattttcatt ataaaaacaa gaaaatatct
      541 agctaaaact aatatatttt atttaatatt ttttatagca ttgtttgcaa actcagtatt
      601 tacgatatct ttataaggtg cttccttatc tagttcttta gcttctttca taacagtcat
      661 caatttcttc aaaccttcct cttttaatac aggattttga gaccatgcat ctattgattt
      721 atatctatct acaactgttg ttaaatcttc taaagatata tcttcaaaga aaggttgcat
      781 agccttagct acttctttgc tagatgcttg ttgaatccat aattgacctt tataaacagc
      841 atttgtaaat ctttgaatta aatctttatt gtttttcatg tatgatctcg tagtagagta
      901 tgctgtaaaa ggtatttctc cagaatcttt tcctatagat gtaactatat atccactacc
      961 ttctttttcc attgctgttg ctgtaggttc aaatgctgtt gtaaagtcac cctctccacc
     1021 tgcaaaagct ccagccatta caccaaattg tacatcagtt cttacattaa cattgcctgc
     1081 ttttagattt tctccaatag taagaccttt ttcttttaga acatattcaa aagtcattag
     1141 tggaactcca ccttttctac cacctaatat ttcggttcct tttaaatcat tgtaagagaa
     1201 attaggcatt tttttcctag caactagaaa actaccatct ctttttgtca attgagcaaa
     1261 gttcactgca taatcgtttt tgttttgatt atatacatat atagatgctt caggacccat
     1321 taaacctacg tttgcttcac ctgaaatcaa agctgccata gttttatctg caccttgggt
     1381 atttataagg tcaatcttta taccttcatc ttcaaagaat cctttcgtaa tagcagcata
     1441 ctgtggagca taaaaaacag agtgagtaac ttctgctata gttactttat ccagattttt
     1501 agacgaagaa gattcatctt ttttgtcatt agagttaaaa tcacatccag ttagacatat
     1561 tgtacttaca atgatagagc ttgctattgc gactattttt ttaaacataa taaatacctc
     1621 ccataagaca tacgaatagt atatagtatt aaatatttat tgtagttgtt acaaaaatgt
     1681 atctatactt ataatatttc tacaaaaact taataataat tttcagtttt atgtttagtt
     1741 ttttcagtaa tgtgtaatat tggataaatt atattgatta tttataaact aagtaataaa
     1801 attaaaat
//
LOCUS       DACULQ010000316         1793 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.318, whole genome shotgun sequence.
ACCESSION   DACULQ010000316 DACULQ010000000
VERSION     DACULQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.318"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            31..405
                     /locus_tag="JA154_18610"
     CDS             31..405
                     /locus_tag="JA154_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7997793.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            402..1265
                     /locus_tag="JA154_18615"
     CDS             402..1265
                     /locus_tag="JA154_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997794.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1265..>1793
                     /locus_tag="JA154_18620"
     CDS             1265..>1793
                     /locus_tag="JA154_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAT7997795.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPIS"
BASE COUNT          687 a          189 c          287 g          630 t
ORIGIN      
        1 actatatata caatttaagg aggtgtctta ttgaatatag ttattagtaa ttcgagtggg
       61 aagccaatat atgaacagat tactacacaa attaaaagta tgattataag tggagagtta
      121 ccagaaggct cagctcttcc aagtatgaga ctattggcaa aagaacttag aataagtgta
      181 attactacaa aacgagcata tagcgattta gaacgagacg gatttattga gacagttact
      241 ggtaaaggca gttttgtatc tagtaaaaac atggattttg tcaaagaaga acaatttcgt
      301 ttaattgaag aatacctaca aaaagcagtt gatacagcaa aaagtagtga tattacatat
      361 gacgaattaa aagatatttt atttttatta tataaaggag aatgataagt atggatgcaa
      421 taactataaa aaatttaaat aaaacttata aagattttag cctacaagat atatcttttt
      481 cagtaccaaa agggagtgta atgggatttg taggtgaaaa tggtgcagga aaaacaacta
      541 cattaaaagc aattttaaat ttaatatctt atgatagtgg aaatatagaa atctttggtc
      601 ttgatagtaa aaaaaatgaa aaagaaatta aagagcaaat tggggttgta tttgagggaa
      661 gcaactttca tgaaaattta aacactgacc atgtttcaaa aatcatgagt aaaatctata
      721 aaaattggaa tgacacatta tttaaagact acttaaaaaa attgagagta ccagacaata
      781 aattaattaa ggagttttca aaggggaata agatgaaact tagtattgct gtagccttat
      841 ctcataaacc aaaattatta atactagatg aggcaactag ttcacttgac cctatcgttc
      901 gtgaagaaat actagatata tttttagatt ttatacaaga tgaagaacac tctattatct
      961 tatcttcaca tattacaagt gatttagata agatagcaga ctatattaca tttatacata
     1021 aaggaaaaat tgtatttagt gaaaataagg atgaattaat tgatactatg ggagttttaa
     1081 aatgtaaacc tagtgatttt gataaccttt caagagagga ttattcatat tatcgtaaaa
     1141 gccaatttgg atatgaagta cttcttaaag ataagcatag atttatctca agacatccaa
     1201 actgtattgt tgataatacg tctatagaag aaattatgtt attttatgtg agaggtgata
     1261 aataatgaaa ggtctaattt taaaagattt acttaatttg aaaggaaata taaagtttat
     1321 acttttattc atcattatgt ttggctttat gtcttcttta ggagatggaa atgttaataa
     1381 ctttataggt gttattatag ttttatgtac tacaatgatt gtttcaacat tttcatatga
     1441 tgatttgaat aaatgggata gttatgtact tacaatgcct attaatcgca atgatattgt
     1501 attaagtaaa tatttaacta tgttaatatt tagctttata ggtgttcttg tttcacttat
     1561 tgttagtgta actattggtt attttaaaaa tacacttata ttaaatgaaa cactacttat
     1621 taatgcacta atcttatcaa tttcagtatg ttttggtagc cttattctac cacttattta
     1681 taaatttggt acagaaagag ctcgtttatt aatgatttta tgctttttag ttccaacact
     1741 agctttatta gtttttaaaa gtattttaga aaatattagt tcacctattt cta
//
LOCUS       DACULQ010000317         1756 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.319, whole genome shotgun sequence.
ACCESSION   DACULQ010000317 DACULQ010000000
VERSION     DACULQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.319"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            164..1594
                     /locus_tag="JA154_18625"
     CDS             164..1594
                     /locus_tag="JA154_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997796.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT          808 a          157 c          311 g          480 t
ORIGIN      
        1 attaacttta ttaccccaca tatataagat aaaattatta atataaaata taatctatcg
       61 acaatgaata gacttttaaa taaacttaat ataaaattaa ttaaataagt gcttaaaaac
      121 gatttacttt aatgatagaa tcatattaaa ggaggataat tttatggata taaagaataa
      181 aattaaatct atatttttac cagaaaaaaa tgactattta gatgatgaag aatttgagta
      241 tgatatagaa aataatgaag aagatttaaa ggaagataac aaagtacatt taaatgattt
      301 aagcagagta aaatatgatt acagagagtt aaaagacata gaaaaccaaa cagaagagat
      361 gtgtctgcta gcagtaaagc aggatggtac tataattgag tttgtaaagg acaagactta
      421 taaagtatgt atggaagcag taaaacagac atataaatct cttaagtata taacaaatca
      481 aaatgaggat atttgtatag aagcagtaaa acagaactac agagctttgt actacataaa
      541 taataagaca gaaaatgtac taattgaggc tataaaaaat gcatcaactt atgatgtcat
      601 ggaagtattt aaatttgtgg aagagcaaac agaagatgta tgtctagcat ttatagaaag
      661 ggcatctaaa aatgatgttg cagagatatt aaaaggaata aaagagcaaa ctccagccat
      721 atgtttagaa gctgtaaaaa aagatggaaa atctttagct tatgtaaagg aacaaagtaa
      781 tagtatatgt ttagaagctg taaaagaaaa ctacagtgca ctatcttgtg taaaagagca
      841 aactgaagaa atatgtatag aagcagtaaa acagaatgac tttgcattgt attatgtaaa
      901 tgaacagaca gaaaaaatat gtatggaagc agtaaaaaga agttatatgg ctctacagta
      961 tgtaaataag caaacagaag agatttgttt ggaagcagta agaattgatg gaagagcatt
     1021 acagtacgta aaagagcaaa cagaagaaat ttgcttggag tcagttagac aaaatggaaa
     1081 agtactgcaa tatgtaaaaa aacagactga aaatatatgt atagaagcag taagaggttc
     1141 atttgaagaa cttgaaataa aagaaatatt aagttatgta aagataccaa cagaaagaat
     1201 atttgtagaa gcagtaaagc aaaatggaaa aattcttaaa tatgtagaaa atcagacaga
     1261 attaatatgt ttagaggcag taagagaaaa ctataatgct ttagcttatg taaaggaaca
     1321 aacagaaaag atttgtttag aagcagttaa tcaaagttat gaagcgctta aatatgtgaa
     1381 agaacaaaca gaagaagttt gtctaaaagc agtaaaacaa gattatagaa tgttgaaata
     1441 tgtaaataat caaacagaaa aaatatgttt agaagcagta aagcaaaatt atagagcttt
     1501 agaatttgtt gataatcaaa cagaaaaagt ttgtcttgaa gcagtaaaac agaatagaaa
     1561 agcactacag tatgtaaaac aaaaacaaag ttaaaaggct aaaatatata gaaaacatat
     1621 gcaaataaat aaaatttgat attttgtaat aaataaaatg ggtatctcaa aaggaacttt
     1681 ttagtttgtg aggtactctt tttatatgaa atgaaatata tttttagcct tttaacaatg
     1741 gggaaaaggt ttattt
//
LOCUS       DACULQ010000318         1735 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.320, whole genome shotgun sequence.
ACCESSION   DACULQ010000318 DACULQ010000000
VERSION     DACULQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.320"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(188..1582)
                     /locus_tag="JA154_18630"
     CDS             complement(188..1582)
                     /locus_tag="JA154_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAT7997797.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT          627 a          319 c          220 g          569 t
ORIGIN      
        1 tgcttaatta aatgaaaata taacaattaa aacaatgtct aagtagatat taattcaaaa
       61 tacaagtaaa tatatttatt tttaaataca aaaaacaaat taccactaat taaattagtg
      121 gcaatttatt gaaaacatat tatcatttta taccattact aaatatctta aataaaacta
      181 cacttaatta aaaccctata taaataccta ttgttaaaaa gactagagat acagctgtca
      241 aaatcaacaa gataggcatt ataaatctta cccattttga aaatggaata tgggctactg
      301 caagtgctcc catcagtact cctgaagttg gagttataaa gtttgtaaga cccacaccaa
      361 attgataagc ggttactatc acttgagccg atatattaac caaatcacca agaggagcaa
      421 gtacaggtac tgtaagtgaa gcaagacctg aagatgatgg aattaaaaat gccaatgcag
      481 cttccaagaa aaatgataaa tatacaaata taacctttgg aagcccacct agaaaggctg
      541 ctgcggaatt taatatagta tctattataa atccattttg agcaattatt acgattccac
      601 gagccaaagc tatagcaaga gctgcagatg ctaaatcttt tgctccatct ataaaacttt
      661 ctgagatttc aatttgagat aatctaccaa ccaatccaga aagcacacct attgcagcaa
      721 aacacattga tatttctgga atgtaccatc cttgacttaa aacaccccaa atcattattg
      781 ccatccctat tccaaatata gcaagaacag atacttcttt aaaagtaaat tcttttattt
      841 ctgttgaagt ttgattaagt attgatttat tatttttatc tatttcatat actattgaac
      901 tttcaggatt ttcctttacc ttcttagcat agtacataac aaatgctata cttattagta
      961 tatatgctat aaaaataact actctgtaag ttactcctga accaggagca agttgagcta
     1021 gtgcctgtgc aataccaact gaaaatggat ttattgttga agcggcggct cctgctgctg
     1081 ctcctataaa tacaattgct attgctgtta gagaatcata tcccaaggat gtcatgagtg
     1141 gaacaaatat catataaaat ggtaatgtct cttctgccat cccaaatgtt gtacctccaa
     1201 gtccaaataa aatcattgat attggaatta taagaagttc ttttccctta aacttagaga
     1261 taaccgaagc tattcctgct tctatagctc cagtcctatt tacaatacca aaagcacctc
     1321 caactattaa aacaaaacct acaacttcag ctgaatcgat aaagccttct attggtgctt
     1381 taaaaacatc tgcaaatcct tgaggattac tttctacact ttgataagtc cctggtacaa
     1441 ctaccattct accttctaca tcttctctac taaattcacc acttggtact atccaagtca
     1501 atattgccat caagactatt agtaaaaata ttattgtaaa agtgtgtggt ataataaatc
     1561 gtttcttttt actgtcagtc attataatgt cctccatact ttactaaatt ttaaatcttt
     1621 ctaggatatt gtttaatctc tctgactaaa gttttccctt tttacaattt tttattagca
     1681 aagatataaa ataaaaaagc taccttttaa aatttaaaag ataacttttt tattt
//
LOCUS       DACULQ010000319         1725 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.321, whole genome shotgun sequence.
ACCESSION   DACULQ010000319 DACULQ010000000
VERSION     DACULQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.321"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     repeat_region   12..1215
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=12..40
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            1276..1704
                     /locus_tag="JA154_18635"
     CDS             1276..1704
                     /locus_tag="JA154_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT7997798.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
BASE COUNT          664 a          167 c          245 g          649 t
ORIGIN      
        1 aaataaatgg agttttatat taactaagtg gtatgtaaat atggatttta gtcgttttta
       61 aatttttctc gtaatagttt tatattaact aagtggtatg taaataccat tcatttatat
      121 ttttatctgt atctcttagc catgttttat attaactaag tggtatgtaa attaagtttt
      181 cttaaaaaag tacttgcttt aaatatgttt tatattaact aagtggtatg taaattttat
      241 tttctcttta ttagtcatta gtttttgcct tcgttttata ttaactaagt ggtatgtaaa
      301 tatgaagaat ttgcgaaact aataagaaaa gatattgagt tttatattaa ctaagtggta
      361 tgtaaatctt tctttatcaa agtaagctaa atttagttcc tccgttttat attaactaag
      421 tggtatgtaa atcgtcatta ctttttattg cttccattaa atttgttcgt tttatattaa
      481 ctaagtggta tgtaaatatc gcaagatagg ctaaatctat caaggagagt tgagttttat
      541 attaactaag tggtatgtaa atgactctgc catccctgta acaaacagtc ttaccgcaag
      601 ttttatatta actaagtggt atgtaaataa atattttacg attagttgat gctataattc
      661 tattagtttt atattaacta agtggtatgt aaattctttt gatttagcgt tgtttgtccg
      721 gaattttaaa gttttatatt aactaagtgg tatgtaaatt cgggctagaa tgtattttaa
      781 accctagttc attgaagttt tatattaact aagtggtatg taaattttct aatatcgcta
      841 tttaaactca atgcttcatt tgttttatat taactaagtg gtatgtaaat tcagaacaaa
      901 ttaaatatgt ttcgttttcc atgtaaggtt ttatattaac taagtggtat gtaaatagaa
      961 cttttttata ttagaagtac ttgacatgat tgccgtttta tattaactaa gtggtatgta
     1021 aatcttgata atatttcagc atatgatact gaagatggtt ttatattaac taagtggtag
     1081 ataagtagaa tattagcaat atcaacgagt atttagaaac ttattttata ttaataaagc
     1141 agtatataaa attgtagaat caacaatagc atatactaaa acatccacct tatattaact
     1201 aaatcatatc taaataaaat aaacttacct ataaacatta taaaatcaat acaaaaatga
     1261 ggtgaaataa aatttatgat aaagaaatta aacaataaag atataaataa aatcatggaa
     1321 atatgggaaa aaagtacaat caaagcacat gactttataa gtaaagaata ctggcaaaat
     1381 aactataata ctgttaaaaa cgaatatata cctatatcag atacatttgt atatgatgat
     1441 ggagatgaaa taaaaggatt tataagcata atagataaaa gctttatagg agctttattt
     1501 ataaagccca aataccaaaa tctaggtatc ggaggtaaac ttttagatta tgcaactaaa
     1561 aaatataaaa gtctaagctt agcagtatat aaagataata aaaaagcagt tgtgttttat
     1621 aataaaaaag gttttaatat agtaaaagaa caagtaaatg aagattcagg atttaaagag
     1681 tacataatgg aatatagtaa ataatatgat tacattctat taaaa
//
LOCUS       DACULQ010000320         1690 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.322, whole genome shotgun sequence.
ACCESSION   DACULQ010000320 DACULQ010000000
VERSION     DACULQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.322"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            119..853
                     /locus_tag="JA154_18640"
     CDS             119..853
                     /locus_tag="JA154_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7997799.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNALMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(996..1487)
                     /locus_tag="JA154_18645"
     CDS             complement(996..1487)
                     /locus_tag="JA154_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAT7997800.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          633 a          197 c          245 g          615 t
ORIGIN      
        1 attattaaaa aatttgttta tagaaacttt tattatttta tataaaagtg ataaaaactt
       61 atattttgta tttaatagag tgttgctaag tgaatatatt taaaatggag gaaactttat
      121 gaaacaaaat aaatatgatg atgatagatt ttttaataaa tacagtaaaa tggaacgttc
      181 tacaaatgga ttagcaggtg ctggagagtg gcatgtttta aaaaaaatgt tacctgattt
      241 caaaggaaaa agagttttag atttaggatg tggatttgga tggcattgtc aatatgcagt
      301 tgagaatgga gcaatttctg ctgttggagt tgatatttca gaaaaaatgt taaaagaagc
      361 tagaaacaaa acgcaacttg ataatataaa ttacatatgt atgcctattg aagatattaa
      421 ttttccaaaa gatagttttg atgtagttat tagttccttg gcatttcatt atattcaatc
      481 atttgaagat atatgtaaga atataagtaa ttgtctttca aataaaggta attttgtatt
      541 ttctgttgaa catcctatat ttactgcata tggaagtcaa gagtggtact gtgataaaga
      601 tggaaataat ctacactggc cagtagatag atattttgaa gaaggagtac gtaggtcaaa
      661 ttttctagga gaagaggtta taaaatatca taaaacatta acaacttatt taaatgcgtt
      721 aatgaaaaca ggatttgaaa tattagaaat tattgaacct caaccagaag aaaaactgtt
      781 agatacaatt cctgatatga aagatgagct acgtagacct atgatgttac ttgtatctgc
      841 aaaaaaaaga taagtaaata taaataatca ttattgtaga aaaaaagttg tagaaaataa
      901 ttacactaat ggagatgatt ataccagtgg tattaattac accagtagta aggaggcaaa
      961 agcattcgtt gcttttgcca ttttttaagt tgcaattata cttctacgta gtatatttcg
     1021 tttccatttt cttctataag ctttttcata tcttcaaatt ttaaaaatac agtagcagta
     1081 ttatcatttg gatgtatacc tattttatcc atttttaata aatccttatc aaatacaact
     1141 tttacaacag cctctttatc atttaatata ccaaaaggag tcacagctcc tttagtaagt
     1201 ttcaagtatt tttgaagtct ttcttctgat gcaaaactaa gaggtttact atcaatttgt
     1261 tgacgaatat cttttaaatc tgcttttttg tctttaccta atacaactaa aaaatgttct
     1321 tttcctttag catcacgaag aaataggttt tttacaacaa attccatatg taatgttttt
     1381 aattcttcca tttcttcaat tgtataaact ggtggatgat ttacagcctc ataaggtatg
     1441 cttaactcat ctaatttttt atatacttcc tgttgttgat tgttcatgat ttcctcccat
     1501 tagaaattta ttttatatat aaataatatt atcacagaaa actttaaaag aaaacgtttt
     1561 atataatata gacaatagat tatattttta taaagaagca agaatactca ataaaactta
     1621 aagaaattaa acgatttcta aaaatacctt gaaaatagaa ccttatttgc catttataaa
     1681 ttatataaaa
//
LOCUS       DACULQ010000321         1671 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.323, whole genome shotgun sequence.
ACCESSION   DACULQ010000321 DACULQ010000000
VERSION     DACULQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.323"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            180..518
                     /locus_tag="JA154_18650"
     CDS             180..518
                     /locus_tag="JA154_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997801.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            540..857
                     /locus_tag="JA154_18655"
     CDS             540..857
                     /locus_tag="JA154_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT7997802.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1007..>1671
                     /locus_tag="JA154_18660"
     CDS             1007..>1671
                     /locus_tag="JA154_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997803.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYY"
BASE COUNT          735 a          162 c          252 g          522 t
ORIGIN      
        1 ctctaattaa aaatatgatt tatccatatt tgtttattgt agatacagac tatacaaaaa
       61 taaatagcac ttatttcagt ataaaaacat atatagtcta tatttagact aaaaaagtat
      121 aaatagtcta tataaaacaa cttaaactat gaatataatc taaagtggag ggataataat
      181 tggcaaatat aggaaaaata ataggagata gaataaaagt ctatagaaca aaattgggat
      241 atagtcaaga gtttctagca gaaaaggcag gtttacatcc aacatatatt ggtcagattg
      301 aaagagctga gacaaataca tctatcaata ttataatgaa aattgctact gctttagaca
      361 taccacttga attgttattt gcaaatatta ttacaacaga aggcgttgat aactctgtac
      421 ctttagaatg ttatgaaatg attaataaat taactgaaaa agaacaaatt gcaatgctag
      481 agttaataaa gtgcatcatt aaatacaaag aaatataagt gagggaaata gtaacaataa
      541 tgaattatat aggtaaaaga cttaaagaag aacggaaaaa agcaaattta acttcaaaag
      601 aatttgcaaa tatggttgga gtatcgcctt ggtatataac tcaaatcgaa agtggtaaaa
      661 agaatccaag tcttaaaaca ttcataaaat ttgtaaatat attaaatatt tctgcagatg
      721 ttcttataaa agatattact tccactggaa aaacatattt agaaaatgat ataaatgaag
      781 agttaaaaga cttaaattca agagaactaa atctgatatc acagattata gacatcataa
      841 aaaagaataa aatataattc tttatatttt atataaaaaa aaaggttatt tgaaataaat
      901 aacctttttt tgtctttttg tgttaacatt gtcttatagt gctaatgagt actattagac
      961 catgttctat ggccttattt taatataaaa cgattggaga taggctatga ttgtgattga
     1021 aggtagcgat aaatttaaga ttgcaaaaga atatattgat gtagaatata ctctttttag
     1081 caaagtaact tttaggtatg aaaagttgaa atttaaagat aatgctgaat tggaaaaaat
     1141 taagatgttt aaatataaaa atggctacat ccctaataaa ttaaaccttt cttttggata
     1201 tggattctct tcttataaaa agcaaataat tagagaaact gtagatactt taagattgac
     1261 agaaattttt tcaagcgaga acatagaaga tattaaattt ataaaagatg gtacaaaaaa
     1321 attagaaatt agcatagaga aagttgtgaa atttaaacgt cgaaaaaaaa gaaattatgt
     1381 ttgttgctat tgccctgata tgtatagaga cataaaactc gacaaagaat ctatcaataa
     1441 gatatacaac agaaaaataa aaatagaaag agaagttaat atttttgaag atgaggatgt
     1501 tataataaac aagaaagtat tgaagtttcc aaagtcttgg acaaagaata tgcaaaaata
     1561 ttggttaagt gaaaataagt atcccataca ttctactgta attgatgatg atagatataa
     1621 atgttgtaat gtacaatata caaaaaatag agtgataata ttatattaca t
//
LOCUS       DACULQ010000322         1670 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.324, whole genome shotgun sequence.
ACCESSION   DACULQ010000322 DACULQ010000000
VERSION     DACULQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.324"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            67..1395
                     /locus_tag="JA154_18665"
     CDS             67..1395
                     /locus_tag="JA154_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT7997804.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          560 a          199 c          316 g          595 t
ORIGIN      
        1 ttggcataat atatgcttat ttaactatga aattaaaatt aaataggatt aatgaggagg
       61 aaaaaaatgg gaagtaatga actgaaaaag actcttggcg tatcagcagc attatcaact
      121 gttgttggtt ccgttatagg agcgggcgta ttttttaaac cacaagcagt ctatacatta
      181 actggaggag ctccaggtct tggtatattg gcatggttaa tagctggaat cataactata
      241 acagctggtc ttacagctgc agaggtatca gttgcgatac caaaaactgg tggaatgatg
      301 gtatatataa aagaaattta tggagaaaaa ttaggttttt taactggatg gatgcaaatt
      361 gtattatttt atccagggat gatggcagca ttaggtgtta tatttggaga acaagcatca
      421 gcactaattg gtagtccatc acttttactt ccaatagcaa ttggaattat agtaatagtt
      481 gcagggctta atatgttagg ttcaaaaact ggaggagtca tacaaactgt ttctacaatt
      541 tgtaagttaa taccacttat tttaataatg gttgttggat ttattaaagg tggtggaaat
      601 aatccaatac ttacacctat ggttggtgaa ggattaagcc tgggtagtgt actaggtcaa
      661 gttttaattg ctatattatt tgcttttgat ggatggatga atgttggtac tttagctgga
      721 gaaatgaaga atcctggaaa agaccttcca aaagctataa taggtggatt gtcagttgtt
      781 atggctgttt attttataat aaatttagcg tatctttggg tattaccagc taatgaatta
      841 gcaaattatg cttctcctgc atcagcagtt gctgaagtta tatttggttc aatgggtgga
      901 aaaataatat ctgtaggtat attaatttca gtgtttggag cattaaatgg atttttacta
      961 actggttcaa gggtggcata tactttagct acagataaga ctttaccaaa gtatggtata
     1021 ttttctaaat taaattctgc tcaagttcca gcaaatgcaa tagcattagt atctgttatt
     1081 gcatctatat atgctttgtc aggtcaattt aatttattaa ctgacttagc tgtatttgct
     1141 acttggatat tctatgtatt aacttttata ggggttatga aacttagaaa gactcatcca
     1201 aatataccaa gagagtataa agttccatta tatcctattg ttcctataat agctatagca
     1261 agtggtatat ttgttgtagt taatcaattg tgttttgcag gtatgaaaac tactatgata
     1321 tctataggag gtcttgtaat aacagctata ggcttaccag tgtatgctta tatgactaga
     1381 ggagtaaaaa gataaaccaa gtagtttaca aatgcttcat aattaaaata aaagagactg
     1441 tctcaaatag agataagtct cttttttatt gttaaaatgg taaagatatg tttaatttta
     1501 caaaaaaaga gcgttccatg aactaaaaag tttattttaa ggtattcatt ttatttttag
     1561 ttttttgatt ataaaattaa acatctaaaa aattatttat tttaaaatac tttattttaa
     1621 agtgtttttt atcaaactaa gtggtatctg tacaacaaat attatacata
//
LOCUS       DACULQ010000323         1665 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.325, whole genome shotgun sequence.
ACCESSION   DACULQ010000323 DACULQ010000000
VERSION     DACULQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.325"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     repeat_region   1156..1645
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1156..1184
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
BASE COUNT          604 a          160 c          244 g          657 t
ORIGIN      
        1 ttacttgttc atatcattat tatatatgat tttctcacag atttaaaagt attataatcc
       61 acttgtagct aagaaaagaa ctctaaataa ttagtgttct tttcttatca acttacgaac
      121 cagttggcaa catgaaatat ttcaacgaca cgtcgcttaa gcgaagtaaa ttttttattt
      181 attatcgtta catagcattt gtaattagtt ttataattta gttaaaaact ttgagatgtg
      241 tctttagatt atctaaagta ttttttaata ttttagatgt attaatttta tttttctcta
      301 aaaagtgtac taatctgtat tttctttgct tttctaagtg cattaaatga agaaatatat
      361 attataaaat atatatttct taatggtgtt agatatacat atattataca agagttggtc
      421 gttgccttga caatgctcct atggaaggat tttggggaat tttaaaggta gaaatgtact
      481 atttaaggaa atttgataca tatgaataat tagttaaagc aatagatgat tatatatact
      541 actataataa ttttagatat caaaatagat taaattctat gtctccattt gaatttagga
      601 aatatttaag tacagagaga gcataattta ttttaagtat ctctaaaaaa attaaatatc
      661 tagggtttta tttgacgtgc tcttttttag atagtaaact ttaaaatata gatattaatt
      721 atatgaatat aataaaaaga gtactaatga gttacactag tactttataa ctatttttac
      781 atgttttaac tgtataaaac agcgggtata gttcacacag gcaggagtga ctttagtttt
      841 gaactaaaaa tcaagttcaa aaagaataaa cattagtatt tgaacttcac tctacgtcta
      901 aatagattgt agttcttctt gcttttatta taccacaaat tggtatagat attcaaaaat
      961 aatatattta tgatataata aaaatgtaaa tagttttgca gtgagcgata tttgttacaa
     1021 agtagagctt aacgcttgaa atataaggtg ttgagggtat atgataagct ttatcatttg
     1081 cactactcat ggttcactgc aaatttaaga gagttgcata tgtgtaagta ttgaaaatgc
     1141 ccagtttatt ttggggtttt atattaacta agtggtatgt aaatttttta attatgttta
     1201 tattaataaa tccaaaatat gttttatatt aactaagtgg tatgtaaatt caaatcgtta
     1261 gactggaagt ataagttgta gtgaccgttt tatattaact aagtggtatg taaatcctat
     1321 attacttaaa ttattactta aatactgata gttttatatt aagtaagtgg tatgtaaatt
     1381 ataaaacttc ttgcatctct gcattaaaaa aagactgttt tatattaact aagtggtatg
     1441 taaatataag gaagaagaat aaaaagggga tgtttttatg gaagttttat attaactaag
     1501 tggtatgtaa atttattatt ataatggtca tgtatatcaa gtttagtagc gttttatatt
     1561 aactaagtgg tatgtaaatt atttcttcct tttgcacata tacatcatct aaattggttt
     1621 tatattaact aagtggtatg taaatatgac taaaatcata gaaaa
//
LOCUS       DACULQ010000324         1639 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.326, whole genome shotgun sequence.
ACCESSION   DACULQ010000324 DACULQ010000000
VERSION     DACULQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.326"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(74..640)
                     /locus_tag="JA154_18670"
     CDS             complement(74..640)
                     /locus_tag="JA154_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAT7997805.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTIGTVLGAVV
                     LGPFWSALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            806..1327
                     /locus_tag="JA154_18675"
     CDS             806..1327
                     /locus_tag="JA154_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997806.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
BASE COUNT          694 a          187 c          246 g          512 t
ORIGIN      
        1 taataaaaat gttatataaa aatgagtata tagcatttta aagagtaata aatcttacat
       61 aataaatgat ttattaatat gaactagaag tatttttact atattgtttt ataaaacctt
      121 taggcatttt acttataaaa tataccacca taatacaaga caaaggtttg tctactatat
      181 tactaagaat ccttggaaag aacgcagcag taaatatctt ctgaccagac tgaactaaaa
      241 aaccaaccaa taaatcagca ccacttccac ttaaaccacc aaacaataaa actgaaatag
      301 gagtaccaat taaaggacaa acaatagata aaattactcc tgtaataata gcttctttat
      361 aaccaaaacc ttttttctta gaaatatatc caacaataag accaagaaca atatttacaa
      421 gggcaaaagg tatatttgta ggacctgata taacacctaa aactaagttt gtacacccac
      481 caataagagc actccaaaaa ggacctaata cgacagcccc aagtacagta ccaattgtat
      541 ctaaaaataa taatggtata tttatcatag atataaccat acctaagaca atattaataa
      601 ctatgcacat tgaagaaaat gtgacttttt tagtatccaa ttataatacc tcctaaaaaa
      661 atatatacat ttatcatagt atagattata aaagattact aattgtatat ttctatatag
      721 aatattggaa atatagaaca aataaataat ataattatca aaatatgtag tatcattaaa
      781 attaagaata actaaggaga ttattatgaa gagaaataga gagattgtag tagtatgtca
      841 ttgtatatta aattgtaatt ctaaagtaga gggattgtca gaatttaagg gagcacaaaa
      901 tatagcgata gatttaataa ataaagggta tggaataatt caactaccat gcccagaaat
      961 gattatgtat ggaataaata ggtggggaca tgtaaaagaa cagtttgaca atttgttttt
     1021 tagagaacaa tgtaggcaaa tgttaatacc tcatgtaagg cagtttgatg aatatttaaa
     1081 aaatgattat gatataaaaa gtatcattgc aatagatggc agtccaagtt gtggttataa
     1141 taagacatgt agttctaaac gttggtttgg tgagttaagt ggttgcaatg aattgatgga
     1201 taaaataaat gatatacaaa tggtggatgg gaaaggtatt tttatagaag agctagaaaa
     1261 gctacttaaa gaatataatt taaatatacc tatagttgga tttgacgagc ttagagaaga
     1321 taagtagaga tataaatatt tattgtctca ttatgaaaca ccattatatt tggcagaaaa
     1381 atatgactga tggacagtac ctgaatcatt tttaagtgga gtaattagta cttataaaga
     1441 tgtatccact agttgaaaga aaatgtagag aggtaataaa tgaattaaga agtagataat
     1501 atatataaag gagtgtctaa aagtgaactt tttagtttat gagacactct ttttttatat
     1561 taaatcaagt atattaaatc aaatatattt taatcatttt agcaatgaaa aataaattta
     1621 tctatatttt gagacaaca
//
LOCUS       DACULQ010000325         1517 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.327, whole genome shotgun sequence.
ACCESSION   DACULQ010000325 DACULQ010000000
VERSION     DACULQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.327"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            481..1188
                     /locus_tag="JA154_18680"
     CDS             481..1188
                     /locus_tag="JA154_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT7997807.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          634 a          137 c          268 g          478 t
ORIGIN      
        1 ttattaagta ttagaaaatg acttcttatt agagtaagta tctaaaatga tataaaaatt
       61 atgattactc attaaagtga ctattataga gtggataaga ttactcatta aagcgattac
      121 tgtggagtag ataaaattac tcactaaaat aactatcata gagtggataa gattactcat
      181 taaagcgatt actgtagagt agataaaatt actcactaaa ataattatca tagagtggat
      241 aagattactc attaaagcga ttgctgtgga gtagataaaa ttactcacta aaataactat
      301 cacagtagat aagattattc attaaagtta ctatcacaga gtggataaaa ttacacatta
      361 aaattgtctg ttaaagaatg aataaaaaag agattgctca atataagaag catataaaaa
      421 ataaaaaatc aaacgtataa aatttaataa ataataactg atagggaaag gaaagataga
      481 atgatattta caaaaagtaa taaatacgat aaggactttc tcatgaaaaa tatgatggga
      541 cctaattgta taaaaatatt ggaagaatta acaagtaaga taaaactaga aaagggaatg
      601 cgtattttag acttaggttg tggtaaaggt atatcttcga tatttttagc aaaggaattt
      661 gatgctactg tatttgcaac agatttatgg atagaaccaa ctgaaaacta taaaagattt
      721 aaagagttta aattggatga taaaatattt ccaatacagg cagaggcaca tgaactacct
      781 tatgcagaag gtttctttga tgcggtgata agtatcgatt cttatcacta ttttggaaac
      841 aaagaaggat ttttagacaa tcatatatca cctcttgtaa aagagggggg aatacttgct
      901 atggcaatgc ctggattaaa agaagatttc gtggattgta taccagatga actcattcca
      961 ttttggcaag ataatatgaa cttccattca attacatggt ggaataaact ttggtctgaa
     1021 tcggagtctg tgattgtaga gaaatgtgaa gctttaaatt gtcatgatga agcttggaag
     1081 gattggataa attgtgataa ttcatatgct ataaatgaca aaaaaatgat ggaagtagag
     1141 aatggaaagt attttaatac aatttctttg atagcgagaa ctaaataatt gtgatgaatt
     1201 tataaaagta taagataagt agaaatataa gttgaatgag agtataagaa gcatagaaat
     1261 ataagttgaa ttaaagtaga aggtaaataa aaatgtaagt taaataaaga gtttatacaa
     1321 aaatgatttt ttagattatt tatacccaat cgagttgctt aggtcagttg ggtattttta
     1381 ttttttaaag ttaaattgca aatactattg tgtgatatat attttaatac taaatgtttg
     1441 ttttataaga aataaactaa tagaaaaaat agttttaaca atgaaagcaa aaaaggtatt
     1501 gatgaagatt aaaaatt
//
LOCUS       DACULQ010000326         1514 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.328, whole genome shotgun sequence.
ACCESSION   DACULQ010000326 DACULQ010000000
VERSION     DACULQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.328"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(151..1443)
                     /locus_tag="JA154_18685"
     CDS             complement(151..1443)
                     /locus_tag="JA154_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAT7997808.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT          536 a          281 c          192 g          505 t
ORIGIN      
        1 tttttgatat tttcttatac tttttccata ttaaatacaa ataattaagt atagaatcta
       61 aaaataaaag gagttatttg tacaaataac tccttttact tttagttaac tcatttattt
      121 acaattgatt atattttaat taaataaaac ttagtttgtt ccaagtgtat tagattgact
      181 ctttgatggt ataaaattgc caattattcc agctattata acaggcacca cccagttaaa
      241 tcctaatgaa ttaagaggta aagagtttac tactggaaca gctactccat aactagatat
      301 aactgttagt atacttacaa gcaatgacat atatactgct cctttgtaca cattatcatt
      361 cttaattttt tcattgaaga aagctaaaat tattaaaaca attgatggtg gataaactat
      421 acttaatata ggagatgcta tctttattat agttccaaca ccaaaagatg ctactactgc
      481 actgaataca gatacagcta atactatttt ttcataactc aacttacctt ttgtaagtct
      541 agagaaatat tgacctgtgg cagatgtaag acctatagat gtagttaaac atgctaatga
      601 tactactact gctaaaatcg ctttcccaac atttccaagc aatccttcag ttatatttac
      661 tattacttgt gattgtactg catctgttcc atacattgtt gatacagttg ctccaaggaa
      721 acataatcct ccataaacta gagctaatcc tccacaagca agaacacccg ccttaccaat
      781 catttttaac ttgtcactaa tagtagaata tcctttatca tttaaagata ctattagaac
      841 tgatgcaagt gctattgctg caaaagcatc cattgtctga tatccttgac ctataccttc
      901 tgcaaataca ttaggtatca taggtttatc tactatatct ccaagaggtg atataatacc
      961 ttttataatt ataaatgcta aagctattaa aagagctgga gttaaaaact gtccaactat
     1021 atctacgacc ttagaaggtc ttattgttag tacatatgaa agcgcaaaaa aaagtataca
     1081 gaataataac actggaattg aagttccaaa taaaggcatt atacccattt cataagttgt
     1141 tgcacctgtt cttggtatag ctaagaatgg tcctatacat aaaatatctg cacaacctat
     1201 aagaatccct aacttcatac cacatctttt aaacatttca actacgtttc catcgaattt
     1261 agctgtagcc attactgcta gaagtgccaa tccagcatca gcaaacgtaa atcctaagaa
     1321 ggcaattaac cattctggac ctgtaattat tcccaagtaa ggtggaaata ttaagttacc
     1381 tgctccaaag aacattgcaa ataacgcaaa tccaaatact ataacatcct tggtcttacc
     1441 cattataaaa tccccctttt taaccttatt aaattaataa aatgtctcga ttgtttacgc
     1501 ttgcataagt aagt
//
LOCUS       DACULQ010000327         1506 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.329, whole genome shotgun sequence.
ACCESSION   DACULQ010000327 DACULQ010000000
VERSION     DACULQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.329"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            660..1153
                     /locus_tag="JA154_18690"
                     /pseudo
     CDS             660..1153
                     /locus_tag="JA154_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1253..1406
                     /locus_tag="JA154_18695"
                     /pseudo
     CDS             1253..1406
                     /locus_tag="JA154_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          587 a          207 c          150 g          562 t
ORIGIN      
        1 attttttatg catttttctt taaaatcata ttttctgatt caactcttat attataaaat
       61 ttctttacta aattattctc tttttctata tcttcatata tattttttaa tcgcctttta
      121 cctaatcttt tatctaacat tactaatgat ttaactaata tattatatga ttttatcgca
      181 tcttctattg agatatttaa acatatattt actgcttcat aaaaatcaaa ttcattaaaa
      241 aatccatttt tatttacaat cttatatccc tttgcatctc tttctaaata ctcttcctaa
      301 atactcttca aaactttcat catctaatct aaatcctata tcactatgaa aaataggttt
      361 tgcatttgga tatagttcta ttgttttatc aaaagtttcg aataccaagt tattattatt
      421 cgattttcca acagtccact ataaacaata ataagtttgt tgctccaaat aatgtaacac
      481 ttgtagaaag atgttacaga tatataaaag atagagaatg tagtttttct cctaatacta
      541 ttagaaatag aaaaaatatg attaaaaatc aaattgaagt tttctttaaa gatatgaaat
      601 taatagatat aactccttct attttacagt cttatataaa taacatatac aatgagcaca
      661 tgttaaattc aacaaaaaac caagtcgatt ttataaaatc agtattgaaa gaatcttata
      721 gattaaaaga aatatcagaa aatatttgtg attttgttac aaccccaatc aaaaaaaata
      781 gttcaacttc taagctttat acaaaacaaa aagctcaatt attattagaa aaatcaaaaa
      841 atattcctat aggtatacca atttttttga tgctaactct tggattacgt tttggtgaag
      901 ctgtatcact tatatggtca gatgtagacc ttgataaaaa atctcacatg taaatcaaac
      961 attagtttat gtatacaata aaataatctt caaaggccct aaaaccccta gattcaaaag
     1021 aagattattt gttcctgatg aacttataag tttgtttaaa ggggaaaaat taaaacaaaa
     1081 taagttaaaa ttacagggta tattgaaaaa tgaatttgat ttgatatgct taaataaaag
     1141 attcaacccg tgagttcaac caacttttgt tgttcatttt aaaaaactat taagagataa
     1201 taatcctaga gatataaaag tacatgaatt aatacatgca aatgcgactt tgatattact
     1261 ttcaggtact aacataaaag ctatttataa aatattagta catgcagata taaaaatagc
     1321 tatgaatata tattccatat tctcacttat tagatgaaat gaataaagaa acatctgaaa
     1381 atataagcaa aatactattc aaataatgtt cagttttatg tcaattaaat gtcactcaaa
     1441 gtgctttttt tccatttgtc agttaaatgt cacttaaagt acctttttcc tatttgtcag
     1501 ttaaat
//
LOCUS       DACULQ010000328         1479 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.330, whole genome shotgun sequence.
ACCESSION   DACULQ010000328 DACULQ010000000
VERSION     DACULQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.330"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            231..>1479
                     /locus_tag="JA154_18700"
     CDS             231..>1479
                     /locus_tag="JA154_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAT7997809.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVDVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     MSIINTEYREDGAILDVECSNIEY"
BASE COUNT          619 a          133 c          275 g          452 t
ORIGIN      
        1 tttgtaagga gtttttttat tgaaaaaact gcataatata tttgggagga ggtgtaagaa
       61 agtgattttt tatataagat tgagaaaata atgcacgatt atggttatta taatagtaaa
      121 taagaagtgt catttaagga taagataaaa agcaaaattc ttgatgaatt taattataga
      181 gccataatct cgtgctatag attaaataaa aaatagcaga taaggagaat atggatatgg
      241 cgaaaaaagg aataacagta ggattaaaca taaataataa atctgaggac tttaatgaac
      301 taatgattga acttgaaaac ttatgttcag catgtgatat tgatgttgta gggagtataa
      361 ctcaaaatgc aaagcaggtt aatagagcat tttacattgg gacaggtaaa gttgaggaaa
      421 tattaaactt aataaagaaa gaaaatatag agatagttat attttacaat gaactttcta
      481 catcacaact aaaaaatctt gaagaaaagc ttaattgtga aattatagat agaacggcat
      541 tgatacttga tatatttgca caaagggcaa aaactagaga agctaaactt caagtagaag
      601 tagcaagttt aaaatatatg ttaccaagac ttataggttc aaatgaaaat ttaggacgtc
      661 aaagtggtgg agttggtact aaaaatagag gttctggtga gaaaaaatta gaacttgacc
      721 gtagaagaat agaagaaaag ataacatctt taaataggga gttggatgat ttaaaattcc
      781 aaagagaaac tcaaagaagt atgagaagaa aatcaaatct tccaaatgta gctctagtcg
      841 ggtatacaaa tgctggtaaa tcttcaataa tgaataaact agtagatata tttaaaaact
      901 ctgaagaaaa gaaagtgttt gagaaaaaca tgttatttgc tacacttgaa acttctgtca
      961 gaaatatagt tcttgcaaat aacaaagaat ttttattatc tgatactgtt ggttttgtaa
     1021 gtaatctacc acatgactta gtaaaagcat ttaggtcaac attagaagaa gcatgtgagg
     1081 cagacgtact gttacatgtt attgatattt caaatccaag ctataaaagt catattaaag
     1141 tgacagaaga tactttaaaa caaataggtg cagatggtat acctatgata catgtgtata
     1201 ataaaattga tttaatagat gtagacgtat tggatagaat tttagatagt attgataaag
     1261 aaggtatatt tgtttcagtt aaaaaagata taaatataga taaaatgata aaatgtattt
     1321 gtgatagcat atttaaagat tatgtaagat gtaagttctt aattccatat gataaaggtc
     1381 atgtagtttc ttattttaac gaaaatatga gcattataaa tacagaatac agagaagatg
     1441 gagctatttt agatgtagaa tgtagcaata tagaatata
//
LOCUS       DACULQ010000329         1467 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.331, whole genome shotgun sequence.
ACCESSION   DACULQ010000329 DACULQ010000000
VERSION     DACULQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.331"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>1467
                     /locus_tag="JA154_18705"
     CDS             <1..>1467
                     /locus_tag="JA154_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAT7997810.1"
                     /translation="ITSSTDKVLIIYDSKKETAYNRDILNIMRTLLGRFSSDIELLKL
                     SNYDGEINKNYYSHIFILGINENSYNNDKNTKNLISSLNSYKGTICWLGYGIENLLEH
                     KKYNLDYVGKTNNIVSVNYRGKSYNLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYI
                     INDKNLFYVSKLDLDGVLFYIFCDSLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVE
                     IADYLSSKNIPFTIALIPAYVNPKNHKVITLSESPEIVKAIKYMQDKGGTVILHGYTH
                     QYKKEEVSGEGYEFWDGKKDEPLKENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYA
                     MESEGYKELKKYFSTYMGQHQNNDKKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDK
                     FTFQNIKENLNKLSIVRGFSGGFFFHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWV
                     KVDEIQIRNNGDEIIVNYDKDLDEITKSDTRFKSISNIS"
BASE COUNT          625 a          135 c          234 g          473 t
ORIGIN      
        1 attacttcca gcacagataa ggtattaatc atatatgatt ctaagaaaga aacagcttat
       61 aatagagata ttttaaatat tatgaggact ctattgggaa gatttagttc agatatagag
      121 ctattaaaat tgagtaacta tgatggtgag ataaataaaa attattacag tcatatattt
      181 attttaggaa tcaatgaaaa ctcatataat aatgataaaa acactaaaaa cttgattagt
      241 tccttaaatt catataaagg aactatatgt tggttaggat atggtataga aaatcttttg
      301 gaacataaaa aatacaattt agactatgta ggaaaaacaa acaatatagt tagtgtaaat
      361 tatagaggta aatcatataa cttggatgaa cattatgttt ttaacattgt agaatcaaaa
      421 gatacaagta ataaagtaat aggaagtatt aatgatacct taaataagta tccatatata
      481 ataaatgata aaaatctttt ttatgtatca aaattagatt tagatggagt tttattttat
      541 atattttgcg attcacttaa tgatattttt aatattaaga cgtttgataa aggacgtata
      601 tttgtaagaa tagaagatgt acatgctttt agagaaccaa aaaatcttgt agagatagcg
      661 gattatttaa gtagtaagaa tataccattt acgattgcat taataccagc atatgtaaat
      721 cctaaaaatc ataaggtaat aactttatca gaaagccctg aaattgtcaa agcaataaaa
      781 tatatgcaag ataaaggtgg aactgtgata ttgcatggat atactcatca atacaagaaa
      841 gaagaagttt ctggagaagg gtatgaattt tgggatggta aaaaagatga acccttaaaa
      901 gaaaatatga agatatttgt caaagataga gttttgaatg gtcttagagt atgtatagag
      961 aatggaattt atcctcttgc atttgaagcg ccacattatg caatggaatc agagggatat
     1021 aaagaattaa aaaaatattt ttctacttat atggggcaac atcaaaataa tgataaaaaa
     1081 tttagcacaa atacatatcc ttatattatt agggatacag aggagtttaa tatatttata
     1141 cccgaaaatt taggatatat agacccagaa gataagttta cttttcaaaa tataaaagaa
     1201 aatttaaata aattatccat tgtaagaggt tttagtggag ggttcttctt tcattcatat
     1261 ttaaacatag agtatctaaa aaacacaata gaatatttag aaaaacagaa tatagaattt
     1321 atgaatttaa gagattttaa taattgggta aaagttgatg aaatacaaat aagaaataat
     1381 ggagatgaaa ttatagtcaa ctatgataag gacttagatg aaataactaa aagtgatact
     1441 agatttaaat ccatatccaa tattagt
//
LOCUS       DACULQ010000330         1466 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.332, whole genome shotgun sequence.
ACCESSION   DACULQ010000330 DACULQ010000000
VERSION     DACULQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.332"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            44..1384
                     /locus_tag="JA154_18710"
     CDS             44..1384
                     /locus_tag="JA154_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT7997811.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCV
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          564 a          112 c          244 g          546 t
ORIGIN      
        1 aaatataaaa aaataattta gtaatacgtt aggagaaagt attatgataa agtcaagctt
       61 attttggaat attttcattg cgatatcctt tatgatagaa tggtttcttt gtaagaaaat
      121 gctagattat acaagtgaga aaaggatttc taacaaaaaa ataaactttt gtgtggtagg
      181 tattattgtt tttgctgaca taatatattt ttttgatgta cctccaaata taagagtaat
      241 tatatctatg ataattacta ttgcatttta taaaatctca tatgatgtgg ggtttataaa
      301 gtctgtatta atatccttaa tttattggat gttactaatc gccacagatg ctttgagtat
      361 atctttaaca gtttggctta attctataaa tgacatgagt agtatgggga cgggagatat
      421 atacagaatt gagtctataa tattgggaaa gatacttttg attgtgtctt tgcttttata
      481 tagagcctta aagataaaac ttaatataga aataaataaa aggacattat tttatatagt
      541 tattcctata gtcgctaata tagcatcttt ttttgtaatt tttgaataca tatttaatcc
      601 agggtctggt agcttaatac ataatttcca attcgttatg atttctgttg tattgcttat
      661 atcaagtatc actttagttt taattgctaa aaaattacag caatataata ctttagttgt
      721 agaaaataat atgattaaaa atactttgaa gtatgaaaat gagtattatg aagatataga
      781 aaagaaatat ttaaaaacta gattactaag tcatgatatg aaaaatcaca tcttgtgtat
      841 aaatgctatg attaaaaaag gtattgatgt tacttcttat ttaaataatt tgcaggtaga
      901 aattcagagt aatgacttgt tatttaatac tggtaatttt atattagatg ctatattaag
      961 tgaaaagaaa gaggtttgtg ataagaatga tatagaacta aaaataggaa taaattttag
     1021 ttgttgtgaa gctgttgaat tagtggatat atgtcatata ttttcaaata ttttagacaa
     1081 tgcaattgag gcatgtaata aaattgatag tggaaataga gttatttgtg ttaaaggtga
     1141 tgttatgcaa aatttttatt taataagagt tgaaaatact aagatgaata agattattac
     1201 taaaaatgga gatattttga cagacaaaaa aggttctctt catggacttg gaataaaaag
     1261 tgtaaaaaac tctgtaaata agtatggtgg taatgttgta attgaatatg atgataaaaa
     1321 atttgtagtt aaaattgcta ttccacttag aaatgtgaag aaagcagtta atcatgtgat
     1381 ttaaatgtat ataaatttga tgtgtagaaa tttaatatat aagaattaat gtataaaagt
     1441 gatgcataga agtttaatat ataaga
//
LOCUS       DACULQ010000331         1439 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.333, whole genome shotgun sequence.
ACCESSION   DACULQ010000331 DACULQ010000000
VERSION     DACULQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.333"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            165..>1439
                     /locus_tag="JA154_18715"
     CDS             165..>1439
                     /locus_tag="JA154_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997812.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYV"
BASE COUNT          609 a          178 c          165 g          487 t
ORIGIN      
        1 aagttatttt aataacattt aaccagtttt ttatgtaggt aacatttcta catttttata
       61 aattttttta cgttttttaa ttttataatt aaaaatttaa attttataca tattataata
      121 aattgtgata tatataaaat aattaaagat aaaaggagta ttttatgaac atacttggat
      181 taatagttga atataaccca tttcataatg gacacatata tcatttatct aaatctatag
      241 aaaaaacaaa tgcaacacat actgtagcaa taatgagtgg aaattttcta caaagaggcg
      301 aaccagcttt atttgataaa tacactagag caaaaattgc tgtcgaaaat ggtgtagact
      361 tagttataga attaccaact ttatttgctt gtcaaagtgc tgaaattttt tctcatggtg
      421 ctataacaat tttaaattca ctaaactgca taaattctat atgttttggt agtgaagaag
      481 gaaatacaga aattctttat aatatagctg aaattttagc aaatgaacca aatgaattta
      541 agttactttt aaaaaaatat ttagatgatg gtctgttatt tccaacagct agagctaatg
      601 cactttttga ttatatacat caagacaaaa aaaataattt tatacaaaat tattcaaagg
      661 aacaattatc aaaaacatta aattcatcaa acaacatact gggtatagaa tacataaaaa
      721 acctcatcca tttaaacagt agtataaaac catttactat ctccagggtc caatcagagt
      781 ataattccga agaaattgag agtgatattt gttctgctac tgctataaga acttcactta
      841 aagaaaatac agaccttttt catttaaaaa atgttattcc agaaaaaact tataacatcc
      901 taaatgaaaa aatagataat ggttttttac ctatgtttga tgacttcttc tttgatattt
      961 taaaaacaat tatacttaga gatatggaca ctttaaaaga gtattttgaa gtaaatgaag
     1021 gcatagaaaa cagaatctat aaagatatct ttacatctga atttctacat gaactacaat
     1081 tagcaattaa atccaagcga tacactttaa ccaaaatcaa acgaactcta aacaatatat
     1141 tgttaggtat taaaaaaagc gacatgcaac ttataaaaaa catagatact ataccttata
     1201 taagaatact tgcttttaat gataaaggta gagaaattct aaaagagata aaaaataaat
     1261 ctgaaataaa aataataaac aagttttcaa atatatcatt ttcattagat gatacgattt
     1321 ttaaaacact cataagttat gatataaaag ctacaaatat atataattca atatattata
     1381 aaaacaataa agatttagta aaaggcccta tggattttta tactcatcca atatatgta
//
LOCUS       DACULQ010000332         1363 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.334, whole genome shotgun sequence.
ACCESSION   DACULQ010000332 DACULQ010000000
VERSION     DACULQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.334"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            18..1127
                     /locus_tag="JA154_18720"
     CDS             18..1127
                     /locus_tag="JA154_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAT7997813.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            1157..>1363
                     /locus_tag="JA154_18725"
     CDS             1157..>1363
                     /locus_tag="JA154_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HAT7997814.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEY"
BASE COUNT          555 a          176 c          215 g          417 t
ORIGIN      
        1 atatcaagga ggatattatg tcagaagtaa ttttaaaaaa tatatcaaaa ctttactcaa
       61 atggatttaa tgcagtaaaa aatataaata tagacattaa agataaagaa tttatcgttt
      121 tagtaggacc atctggttgt ggtaaatcga ctacacttag aatgattgct ggtcttgaag
      181 aaatctctga aggagaacta tacattggag ataaacttgt caatgatata gaaccaaaag
      241 atagagatat agctatggtt tttcaaaact atgctttata tccccactta tctgtttacg
      301 aaaacatggc atttgcactt aaacttagaa aattgcctaa agatgaaata gacaaaaaag
      361 ttaaagaagc tgctaaaata ttagacttac tacctctttt aaataaaaaa cctaaaacat
      421 tatctggagg acaaagacaa agggttgcct taggacgtgc aatagttaga aatcctaagg
      481 tattcttaat ggatgaacct ctatccaact tagatgcaaa acttagaact gctatgagaa
      541 cagagataac taagctacac caacagcttg gaactacatt tatctatgta acacacgacc
      601 aagttgaagc tatgactatg gctgatagaa tcgtagtaat gaaggatgga gtagttcagc
      661 agattgcaac tccacaagat gtatacgatt atccagctaa tatatttgtt gcaggattta
      721 ttggagcacc acagatgaat tttattgatg taatattaat agaagaaaat gatgaaattt
      781 atgcacaaaa tgaacatatt aagcttaaat tgaataaaga aaaacactat gatttaataa
      841 aagacaatta tattaataat gaagttgtta taggtatcag accagaagat attcatgtag
      901 aagatacttt tattaaatct aatccagata cttgttttaa atcaagaatt gaaataaaag
      961 aacttatggg agcagaaact tatgctcact taaaactagg cgaaaatacg ataacaatta
     1021 gatttgacag taaaaacaga attaatgttg gtgatgactt aacactttca gttgacaata
     1081 gtagagtaca catatttgac aaagaaacta ctttagctat aagataagaa tataatctta
     1141 tttttaagga gactttatgg aagagttaaa agatttttta gaaagacagc ttaataaaaa
     1201 aataaatatc tatccatatg aaaaccaaaa gttagatgct agtagccatt tagttacttt
     1261 caataatgct atttatgtta ttgattttct agataaaaat aatctcgata atttaaaagg
     1321 caacttttat ttaatttacc aatctcatat agactttgaa tat
//
LOCUS       DACULQ010000333         1358 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.335, whole genome shotgun sequence.
ACCESSION   DACULQ010000333 DACULQ010000000
VERSION     DACULQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.335"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(218..532)
                     /locus_tag="JA154_18730"
     CDS             complement(218..532)
                     /locus_tag="JA154_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997815.1"
                     /translation="MMKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSI
                     ISRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNK
                     TK"
     gene            complement(585..1178)
                     /locus_tag="JA154_18735"
     CDS             complement(585..1178)
                     /locus_tag="JA154_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HAT7997816.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          484 a          196 c          172 g          506 t
ORIGIN      
        1 taaagaagta taataaacta atttaagaaa tatagaaaat taatttaaag aaatacaata
       61 cattaatttt taagtaacat taaattaatg taagtaaaat aatgttcata tgaagattta
      121 aaggtgcatt ttctgaagct aagtatatgt tgtagagtgt taggtctctc tatcctgtga
      181 agtttaatag ctcaagaaaa tgcactattt atgatgatta ttttgtttta tttccaattt
      241 tggaaataac tattctaatt aaatctaaaa ttataccata aataagaaaa gaaagtatag
      301 atgctatgta ccaataaatc gaaattgacc ctgtaattgt tctacatggg atttgcatac
      361 ctgaatccat aaaaacaaaa aaactatcaa ctctggaaat aatagacaaa ataggattta
      421 aacctattag aacaatactt tttgagtctt gaccaatttg atgcatataa atctcaaata
      481 atgcaattaa aaaaaaccaa aatgtaaaag atttaataat ttttttcatc aaaatacctc
      541 acataatata aattctgttt gcactatttg cttactacaa ctatttatct ccattcagtc
      601 acatcactta attgtaatct aggtcttctt gatggttctt catcaggata acccaaagat
      661 attgctgcca ctaattgact atctgtattt aaccattcac ataattcacg atatgcaaag
      721 tatacatcac aaatccaaag actacctaga cctaattcca cagctgtaag cgacatattc
      781 tgaattgcag ctccaattga ttgcatgttt gccatttcat agaatcgttc ttcaggggat
      841 agcttttcta aaggtgattt acctaatata tttaaaataa aaattgttac tggagcttgt
      901 ttcatgattt caactgtgta atttgcacca gcgatatgtt gacgacttcc tggaagaagc
      961 ccattatcat taatttcatt ttgaatacct ttgctcatgg ctttaagcat agattctttt
     1021 tccttttcag tgatgacaac aaaacgccat ggttgcctgt ttttagatga aggtgcattt
     1081 attccagctt caattatttt ctctatagtt tcatgtgaaa tgctttgatt tttatatttt
     1141 ctgatacttc gtctttttga tatagaatca cttatcattt taagtaacct ccgtttattt
     1201 tttggaaaaa tctatttaat ttttattttc tattttaatt attttagcat taatcagaaa
     1261 aaaagtcatt gattttagta tacaattatt aatgattata ttaagaattg tatcaaaaag
     1321 tatgtgatta tactaaggga ttatattaaa cattatat
//
LOCUS       DACULQ010000334         1324 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.336, whole genome shotgun sequence.
ACCESSION   DACULQ010000334 DACULQ010000000
VERSION     DACULQ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.336"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            590..1249
                     /locus_tag="JA154_18740"
     CDS             590..1249
                     /locus_tag="JA154_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT7997817.1"
                     /translation="MRVVKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEV
                     SRTPVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEE
                     EEVFQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQ
                     GRFIVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCED
                     E"
BASE COUNT          556 a          129 c          211 g          428 t
ORIGIN      
        1 atctatagta taatagttta atttaaagta caacgattta atctatagta cactagcatg
       61 atttgaagta caacgattta atctatagta cactagcatg atttgaagta caacgattta
      121 atctatagta taatagttta atttgaagta caacgattta atctatagta caataacttg
      181 atttgaagta taacgattta atctatagta caataacttg atttgcaata caatgaatta
      241 atctatggta taatagttta atttgaagta caatgaatta atctatagta taatagttta
      301 atttgaagta caacgattta atctatagta taatagttta atttgaagta caatggttta
      361 atctatagta taatagttta atttgaagta caacgattta atctatagta caataacttg
      421 atttgcaata caatgaatta atctatagta taagagcatg atttacagta caataattta
      481 aattgtagta caaaagtact atcaaataag aatttctact ttaaaaatat tactaaaagt
      541 aattaaagtc cagtaattca atataatatg gcaaaagaat agaaataagg tgagagtagt
      601 gaaagtaaag tcaaatttag atgaaatttt atatcataag attattgaaa gtcttattcg
      661 tggtgaatac tctgtaggtc aaaaaatatt actaaatgac ctttgtgaaa aatttgaagt
      721 aagcagaaca ccagttgtac aagcagtaaa aatgctaaat aaagatggag tgcttactat
      781 tatgacaaat ggtaaagtgt acgtaccaga gtatgaatat gatatggtaa aacaagtttg
      841 tgaaacgaga acactgattg aaacatatgc tttagaaaaa atgatgcaag aagaggaaga
      901 ggtttttcaa caaaagctag atgtaataaa aagatactct gataaatgcg aggagtacta
      961 tcgtcaagaa aaatctgtag aacttgcact agcagatttg gaattacata aagccattgt
     1021 agaaggggca aataatgaga ttttaaatga tgtatatgtt ggtatacaag gaagatttat
     1081 agtagtgaac tatttaattc gaccattaaa aaatcgtaat tatgaaggaa cagtacaaga
     1141 ccattttgaa atattaaaat tcattgaaga cagagataca aaaaaagcag tagataaact
     1201 aagaaatcat attcaaggaa ctattaaaag attctgtgaa gatgaataat aagttttgaa
     1261 gttactatat atacttatct attaagttgc taaatgtagt ctaagtataa tctaattata
     1321 gttt
//
LOCUS       DACULQ010000335         1290 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.337, whole genome shotgun sequence.
ACCESSION   DACULQ010000335 DACULQ010000000
VERSION     DACULQ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.337"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..396)
                     /locus_tag="JA154_18745"
     CDS             complement(<1..396)
                     /locus_tag="JA154_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HAT7997818.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIIN"
     gene            complement(653..802)
                     /locus_tag="JA154_18750"
     CDS             complement(653..802)
                     /locus_tag="JA154_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HAT7997819.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(815..1045)
                     /locus_tag="JA154_18755"
     CDS             complement(815..1045)
                     /locus_tag="JA154_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HAT7997820.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
BASE COUNT          439 a          172 c          146 g          533 t
ORIGIN      
        1 atttataata actttttcgt tgttttttaa agtagcttgc atgcgattat gtgatattac
       61 ttttatactt tttatgtagt ccatatttaa aatcatggat ttagatattc gtgagaagga
      121 tgtattagct aataattttg ataatttata tatttttaaa tcaatttcaa atacatcatc
      181 ttcataataa gcaaatgttt tatttccaac tgtctcaaag tataaaatat cacagacatt
      241 gagtttataa atctctcctg attttttacc ttttatatca agtaacggtg tactcaataa
      301 tgaaattatt ttttcaatgt tattatctag agcagaacat tttatggtta tttcaatatc
      361 atcacaatta ataccttgtt cgacatgtat tttcattaat catcacctca ttttattact
      421 gtcattagaa tagcattata tcttattgag tccaactaga tgcgttctaa atttcaaatt
      481 aagcatagtg aaattcaaaa ataaaacagt gaaattcaaa tttgaggtgg taaaattcaa
      541 aaaaatacta tggatatttg gataaattat ccaaatatcc tagtatttca catagaatat
      601 gtctaattat tttacaaata gacctatttt atgacttaca tatattgtca aattacttct
      661 taaatatcaa aatatttttg gataatgtat caatactgtt tgataaagta tttaatttac
      721 cctcaattct aaccaataag tacatggata gagctatagg aaaaccaaca ttggttatta
      781 acagttgtaa actatcatcc ataaaattag ccccctattc tataactaag tcataaggag
      841 tggtatttgt ttccacaacc ttggcttcaa caacagaagc taggtcaaat cctccaaccg
      901 aaaaaatatt tttagatact atcaaatcca tgacagattt tatattttct tcagttaaat
      961 catcctttgg attttctata gaaaaagaaa cttgtttatc ctttacattt ttaaagctca
     1021 tgactaattt tttcttaatt tccatataat tacctccgat ataataattt atattttaat
     1081 tgtttgcttg ttaatattgg tattttaatt gtcaatatta atgttttgct tgttagtatt
     1141 aatatttcaa ttgtcaatat taatgttttg cttgttagta ttaatatttc aattgtcaat
     1201 attagtgtct tgcttgttag tattaatatt tcaattgtca atattaatgt tttgcttgtt
     1261 agtatcagtg tttcaattgt caatattagt
//
LOCUS       DACULQ010000336         1287 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.338, whole genome shotgun sequence.
ACCESSION   DACULQ010000336 DACULQ010000000
VERSION     DACULQ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.338"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>1287
                     /locus_tag="JA154_18760"
     CDS             <1..>1287
                     /locus_tag="JA154_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT7997821.1"
                     /translation="DTGIKDKYKELFLKIEELDTILNLSKDGILFTSKDGEINTYNSK
                     VKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDKVVVFNKKYINVNKKNIYNRDEK
                     MGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFKDIKTNSPKMFECIDLAKKVSKS
                     DLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVNCAAVPENLLESQLFGYDKGTFT
                     GGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLLRVLQEKQIMPVGSHNVINIDVR
                     IISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPLRERKEDILIIMEDLINKKLVIT
                     PEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLEKDLPPNLRSSDNKNTSLKLKYS
                     KNDILNILEILILNKESDVGIGRGLILKALLDKNLQI"
BASE COUNT          580 a          135 c          196 g          376 t
ORIGIN      
        1 gatactggta taaaagataa atataaggaa ttatttttaa aaatagaaga attagataca
       61 atcttgaatt tatcaaaaga tggtatatta tttacttcaa aagatggtga aataaataca
      121 tataatagta aagtaaaaga tattttagac ataaatgagg atatatatgg aaaatacatt
      181 gaggatatat ttgtagatag tttaaaagtt ttattgagtg agaaagagat actagataaa
      241 gttgtcgtat tcaataagaa atatataaat gtaaataaaa aaaacatata caatagagat
      301 gaaaaaatgg gcacatatta tagtttacaa gaaataacat acataaaaaa attagaacaa
      361 aatctcacta aaaaattaag agaaaaagga caaatagcaa aatatacatt taaggatata
      421 aaaacaaata gtcctaaaat gtttgaatgt atagatttag ctaagaaggt ttctaaatct
      481 gatttaagca tacttatcag aggtgaaagt ggaactggta aagaactcat agctcagtct
      541 atacataaca actcaaatag aaaaaatcaa ccctttatag cagtaaattg tgcagccgtt
      601 cctgaaaatt tattagaaag ccagctattc ggatatgaca agggaacttt tactggaggt
      661 ctaaaagatg gaaaacaagg attatttgaa cttgctaata atggaacaat attcttagat
      721 gaaataggcg atatgccttt agaattacaa acaaaactac ttagagttct tcaagaaaag
      781 cagataatgc ctgttggatc tcacaatgta attaatatag atgttagaat catatctgct
      841 acaaataaga atttagaaca aatgattgat aatagccaat ttagagagga tttatattat
      901 cgattaaata caattccaat aaatatacct cctctaagag aaagaaaaga agacatatta
      961 attattatgg aagatttaat taataagaaa ttagtaatca caccagaagc aaaaaaactt
     1021 atacaaaact atatgtggaa aggtaatata agagaactcc aaaatgttac atcctattta
     1081 aacattatgt gtgaagatat tgtattagag aaagatttac ctccaaactt aaggtcttca
     1141 gataacaaaa atacatcatt aaaactaaaa tatagtaaga atgatatatt gaatatatta
     1201 gagatactaa ttttaaataa ggaatcagat gttggtattg gtagaggatt aattttaaaa
     1261 gcactactcg ataagaacct tcaaatt
//
LOCUS       DACULQ010000337         1286 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.339, whole genome shotgun sequence.
ACCESSION   DACULQ010000337 DACULQ010000000
VERSION     DACULQ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.339"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>1286
                     /locus_tag="JA154_18765"
     CDS             <1..>1286
                     /locus_tag="JA154_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997822.1"
                     /translation="YIINEKNNNLIINYNDKYIYAIYLLNIAINSYLNNTLNPNNHIL
                     TKLDKGMPLLYKGQIVIFKNINNTHITLGHKNKTDTTLIPIDSAYMLITYNGHLEVNS
                     MGKTPASKTFQTKNVLSSLGFSDLKNMTGVINDSTLIILPCKDDISDLVSNIKIKDIN
                     NTYKFTELFPCSYISSTGAETDYPGNHAKQIPLLKFTTNISSAFEIIKSDKSIKNVFI
                     IGDYPIYKHINDFERILNRKRIESVNIVTSNSNISNILDLPNIDNLNIYSWSKDVLLT
                     YSQDFENTSTFQKLWLDKLINKNICTTSVDSNISDLIHQARKSLYYISKYDFDISIKN
                     SLMMCSYSLLKILENTPFNLDFLEETIKLLNLNIATPSAKLENIKYLISSTSPDLELS
                     ALFDDVIVKFERIINELSDNNTKFNTLNKLVSSYKY"
BASE COUNT          524 a          176 c          138 g          448 t
ORIGIN      
        1 tatattataa atgaaaaaaa taataactta attatcaatt ataatgacaa atacatatat
       61 gctatttatt tgttgaatat agctataaac tcatacttaa ataatacatt aaacccaaat
      121 aatcacattc ttacaaaact cgataaaggt atgcccctgc tctacaaggg tcaaatagtt
      181 atatttaaaa atataaataa tacacacata actttaggac ataaaaataa aacggataca
      241 acacttatac ctattgactc ggcttatatg cttataacct ataatggtca tttagaagtc
      301 aattctatgg gcaaaactcc agcatctaag acttttcaaa caaagaatgt tctatcatct
      361 ttaggttttt cagacttaaa aaatatgact ggagtaataa atgattctac actgattata
      421 ttgccttgta aagatgatat atctgactta gttagtaata ttaaaataaa agatattaat
      481 aatacttata agtttactga actatttcca tgttcatata tttcatcaac tggtgccgaa
      541 actgattatc ctggaaatca cgctaagcaa atacctttac ttaaatttac aactaatatt
      601 tctagtgcat ttgaaattat taaatctgat aagtctataa aaaatgtatt tataataggt
      661 gattatccaa tttataagca tataaatgat tttgaaagaa ttttaaatag aaaaaggata
      721 gaaagtgtaa atattgtaac ctcaaattca aatataagca atatattaga tttaccaaat
      781 atagataatc taaatatata ctcttggtct aaagatgttc tgttaaccta tagccaagat
      841 tttgaaaata cctctacttt tcagaaatta tggctagata aacttataaa taaaaatata
      901 tgtactactt ctgttgattc taatatctct gacttgatac atcaggctag aaaatcactt
      961 tattacatat ctaagtatga ttttgatatt tccataaaaa actcacttat gatgtgtagt
     1021 tactctcttc ttaaaatctt agaaaataca ccttttaacc tagatttttt agaagaaact
     1081 ataaagcttt taaatttaaa tatagcaact ccttcagcga agctagaaaa tataaaatat
     1141 ttaatttcaa gtactagccc agatttagaa ttaagtgcat tatttgatga tgttatagtc
     1201 aaatttgaaa gaattataaa tgaattatct gataataata ccaaattcaa tacactaaat
     1261 aaattagtat cttcatataa atacaa
//
LOCUS       DACULQ010000338         1272 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.340, whole genome shotgun sequence.
ACCESSION   DACULQ010000338 DACULQ010000000
VERSION     DACULQ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.340"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            8..454
                     /locus_tag="JA154_18770"
                     /pseudo
     CDS             8..454
                     /locus_tag="JA154_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            447..1079
                     /locus_tag="JA154_18775"
     CDS             447..1079
                     /locus_tag="JA154_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997823.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
BASE COUNT          512 a          127 c          218 g          415 t
ORIGIN      
        1 ttcataaaat atatacaatg ccactgaaag ccaattcaaa attagagatt atataaaaaa
       61 tataaatgca ggatttgctc tatatgaaag aagtgctcaa gatttattaa ctaaattaat
      121 gacccatgaa aataaaatct ctcaaaatca aaaaatcctt ttagatgaaa agtttacctt
      181 agatgactca ataaaaaatc tctcaagtat tattgaaaac ttttctaatg acctacaagt
      241 taaactggat atgatgtttg aaaattcttt agatattcaa gataagttag atgttatgtt
      301 taataactct tttatgaatg atgaagttcc tcttgatagt gaagaattat ttaaggatga
      361 tgttaataat gtatttaatc cttttagtga agaaatttat gagatagagg ataaagaaat
      421 aaaggtcata ggtgaggatg agttaaatga ataataaata tagaagaact gttaacagag
      481 aatttgaaaa gagtagcttt tggccaacat ttacagattt attatctact gtattgatgg
      541 ttgtaattct tatcttattt agctcagaaa gtatttcagg ttctgttgaa caagatttag
      601 ctaagaatgt caatgcttct gttgaagaaa cttttaaaaa aagtggaata cctgtgaagg
      661 tagataaatc taatgggcaa gtaacttttg gtgaaaaaac catgtttgat gtagatagtg
      721 atgttttaaa accagaggct aaagaaatgc taaaaatgtt tgttccaaag tacatagaga
      781 ctatttataa agattatggt gactatatat ccaaaatagt tatagaaggt catacagatg
      841 atgtgggaag ttatatatac aacttagatt tatcgcaaag aagagcatat agcgttgcta
      901 aatttatagt gggagatgaa ataggtgact ataaatacaa agataaagtg actaagcata
      961 ttattgcaat aggaaggtca aaagctgaac tcatcaaaaa tggtgacaat agcgtgaata
     1021 gagatgcatc tagaagagta gaactaaaat acgaaataaa tattaatcaa aataaataat
     1081 taaattttta taaaaaaaaa gaaggcagtt ggtgtgagct ttaagccttc tttaaggaaa
     1141 aagtttattt atttttgttg atacttaaca tattatagtt tttagaagca gtttgcaata
     1201 tattaaacgt aaatggtcgt ttaaaatgca taactggata ctactgatgt aaaatatttc
     1261 attttatata at
//
LOCUS       DACULQ010000339         1268 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.341, whole genome shotgun sequence.
ACCESSION   DACULQ010000339 DACULQ010000000
VERSION     DACULQ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.341"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            164..>1268
                     /locus_tag="JA154_18780"
     CDS             164..>1268
                     /locus_tag="JA154_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAT7997824.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKV"
BASE COUNT          549 a          109 c          186 g          424 t
ORIGIN      
        1 tgtgacatat attttaaatg cttattctta aatttttgca ttatttatta aaatttatta
       61 tgctttaacg ctaaattgag tttgataatt ataaaacaaa taatcaaaaa tagtgtaaaa
      121 attaatactt tttgttaata ttaatagata aagataatct aaggtggtga ttaaactgac
      181 aatagaagaa tacaaaaaca aatactttac tggaaataaa atcgtacttt ctataaatag
      241 ttttgataat aaagaactat actatcatga aactgaaaat atattaaatt taataaatga
      301 aatagataaa ttaacatcaa gtaagatatt taaaacaata gaagataaaa atgatttggc
      361 actagagtct tattattcta gtgtaataga aggtgctttt tcaactagaa agattgcaaa
      421 atctataatt agaggaaaga tgaaaccaag taataaaagt gaatatatga tttacaataa
      481 tcatagagcc ttagaatatg gattagacaa cttagataaa ttatatagtc acaaatttat
      541 atatgatttg catcatatat tgggcgaaaa ctgcttggat tcagaagaat atgagtatag
      601 aactgaaaaa gtatatgtat gtgattcaaa aggtgagatt atacatacag ggcttgaacc
      661 tttgaagata tatgacttta tgagtaaatt aatagatttt atggaaaata gcaaagtaag
      721 taatttaatt aaaagtgcta taattcattt ttactttgta tatgttcatc cttttagtga
      781 tggtaatgga agaacatcaa gagccttatc atatctatat ttgattgata aaggatatga
      841 tactttcaag gatttttcaa tatcatatat gatatctaaa aacagaacta agtattataa
      901 ggctatactt gatgtagaaa acaaaggaaa taatttaact gtatttatag aatttatgct
      961 caagtctata atacaatcta ttaatgagat gcgtaatatg catgatagaa aatctttaga
     1021 aagtatatta aaagaagaat tgtttgaaaa tgacataact ctatcagcaa cagaagaaca
     1081 tatattaaaa tatatttgca ataaagataa ctattctatg acattagaaa attatataaa
     1141 aaagaataaa agtagatact taaaggctgg aataaaggaa attgaattag ttgaccagtt
     1201 gatggaagtg tttaataatc ttgaagagat agagattttg tctaaagaaa aaaatatata
     1261 taaagtta
//
LOCUS       DACULQ010000340         1169 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.342, whole genome shotgun sequence.
ACCESSION   DACULQ010000340 DACULQ010000000
VERSION     DACULQ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.342"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            106..603
                     /locus_tag="JA154_18785"
     CDS             106..603
                     /locus_tag="JA154_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT7997825.1"
                     /translation="MDAETLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            653..>1169
                     /locus_tag="JA154_18790"
     CDS             653..>1169
                     /locus_tag="JA154_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAT7997826.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIR"
BASE COUNT          484 a          105 c          215 g          365 t
ORIGIN      
        1 aaatttattg acataatagt aactaattac tataatataa agtaattagt tactatttaa
       61 aattatcaaa aaattaaaat ctaagataaa gagaagtgta taataatgga tgcagaaact
      121 ttaatgaaaa aatatatggt agatgatgat agtagaaaac aagctatatt tgcaagtata
      181 tttgtaatgc aaaataaact tcaaactaca tgtgataaat tagacccaga attaacaatg
      241 aaacaatggt tacttttagc tattgcggac tctgtagatg aaactcttac attaaccaaa
      301 cttggagaat taatggggtg ctcaagacaa aatgtaaaaa aactagctat tgctttagaa
      361 aagaaaggtt ttatacaaat tgagcagaat gaaaaagatt caagagctat ctgtcttgtg
      421 acaaaagata gagtgcaaga gtattctaaa aaagtaagag gtatgcagga aaaagtatta
      481 cagcttttat ttgaagattt tacagagaaa gaagtggaac agttattttt tggaattatg
      541 aaattaagta taggaattga aaatgtgaaa aattatgtag aaaataatga cgttggagaa
      601 taagcaagta tcaaatcaac tttgtatgac atcagagagg gggataattt atatgaaaaa
      661 tgtagtagtg atttataaga gtaagtatgg ttcaacaaaa aaatatgcta aatggctttc
      721 agaattattg tcttgtgata tttttgaaac taagaatatt aatatagaaa aggttatgaa
      781 gtatgatacg attattttag gaggaggtct ttatgcttgt aatatagcgg gaatttcttt
      841 cttaaaaaaa aattataaag aattgcagaa aaaaaattta gtagtatttg gcgtaggagc
      901 tgctccatat gatgatgaag taataaagaa attaagagaa gtaaatttta aggatgaatt
      961 aaacaaaatt ccttgctttt attgtcgtgg ggcatgggat gagacaaaaa tgtcttttat
     1021 agattatact ctttgtagtt tttttaaaaa atctatagct aaaaaagaac gtcaaggtct
     1081 taaaccagca gaagcacctt tattgtctgg agaagcattt gattggtgta ataaagaaaa
     1141 tttaactcca atacttgatt atataagag
//
LOCUS       DACULQ010000341         1163 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.343, whole genome shotgun sequence.
ACCESSION   DACULQ010000341 DACULQ010000000
VERSION     DACULQ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.343"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            186..>1163
                     /locus_tag="JA154_18795"
     CDS             186..>1163
                     /locus_tag="JA154_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAT7997827.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRT"
BASE COUNT          483 a          136 c          224 g          320 t
ORIGIN      
        1 ttatttacac taaaaagtac atactaaatg aggggtaaaa taaagaaaaa tgatatacta
       61 attgtctttt gataataaaa aaacatggaa tttgattatt taatcaagaa aaagtattga
      121 acaaatgatt tatttagtgt acaataatca tgtaaaaaca tagtaatgga gggagacatt
      181 tcataatgaa aattaaggta ggaatgaacg gatttggaag aataggaaga gctgtattaa
      241 gaatagctca agaagaatta ggagataata tagaaattgt agccataaat gcaagagcaa
      301 caacagaatc tttggcacat ttatttacat atgattcatg ctatggaact tttagaggtg
      361 aagtagaggc aaaggatgaa gacactctag tagtaaactc taaagagata aaaatattaa
      421 gatataatga cccagaagaa ttaccttgga aagaattggg ggttgatata gtaatagaat
      481 caacaggatt atttacacaa agagaaaagg cagaaaaaca tattaaagca ggagcaaaaa
      541 aagtaattat aacagctcca ggaaaaaatg aagatataac tatagttatc ggagttaatg
      601 agaaagaata tgataatgaa aagcacaata taatttcaaa tgcatcttgt actacaaatt
      661 gtctagctcc ttttgctaaa gtattggacg aaaaatttgg tatagtaaaa gggttaatga
      721 ctacagtaca ttcatatact aacgatcaaa gaatattaga taaaagccat aaagatttga
      781 gaagagcaag agcagcggct gaatctataa tacctaccac tacaggagca gcaaaagctg
      841 tgtcaagagt actaccacaa ttagaaggaa aattaaatgg tttttcactt agagtaccaa
      901 caccaacagt ttcacttgtt gatttagtgt gcgaacttaa agaaaacgtt acatctgagc
      961 aagttaactc tgtgttaaaa gatgctgctg aaggagaatt aaaaggtgtt ttaggatatt
     1021 gtgacaagcc acttgtttct atagattata aaggagattc aagatcatct attatagacg
     1081 ctctatccac aatggttata gaagacaata tggttaaggt agtttcttgg tatgataatg
     1141 aatggggata ttcttcaaga act
//
LOCUS       DACULQ010000342         1106 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.344, whole genome shotgun sequence.
ACCESSION   DACULQ010000342 DACULQ010000000
VERSION     DACULQ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.344"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            249..>1106
                     /locus_tag="JA154_18800"
     CDS             249..>1106
                     /locus_tag="JA154_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997828.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT          418 a          135 c          183 g          370 t
ORIGIN      
        1 atagagaaaa ttttcataga accatcttta taggggatac tttataaaag aataaaaaaa
       61 ttataatata taaaaacttc ataggagagt aacaacatca gtaaaaatat gaaaacacta
      121 tataaaaatt caaaaatatt aagaataatg aaatacttga aattgcttat atcaagaaat
      181 tttatataat ataaactggt caaatatgtt ataatattaa attgtgacaa gttcatggag
      241 gtgtttatat gaaaaaacaa aagtcgccaa ttttacttga aatgcttgga ctattttttg
      301 gttgtatatc aatgagtatt ggtataaata tgtttttaaa accacataca attgctcctg
      361 gaggacttag tggtctatca ttagtactaa acaaggttac aggacttcca gtatcagcta
      421 tcatgttaat tataggtgtc ccattagtaa tacttgcttt tagaattatg ggtacaaaaa
      481 attcattaaa aactttattt ggaacagttg tattttcagc tatagttcaa ttaacagacc
      541 cattatcaaa actaagattt actaatgact tattattgtc ttctattgca ggaggaatcc
      601 tagttggtat aggtttagga ataatgttta aatcagatgc ttctactgga ggaacagact
      661 taattgcact catacttagt aagaaattcc caggaataaa agctacaaaa ttcatgtcat
      721 gcctagatgg aatggttgtt atatcttctg gtatagtaaa tcgtagttta gaaactgctc
      781 tttactctgg tatagcactt tgtgtattaa taaaaatagc agatatgata atggaaggat
      841 ttgaccattc aaaagcattt tttattatct ctgatgagcc tgaaagttta agacaagcta
      901 taactgaaga acttgataga ggtcttacta ttcttgatgg taaaggtgga tacactagag
      961 aaaataaaga agttctatta gtcgtagttt ctaaaaaaca agaactttat ctaaagagat
     1021 tagttaaaag aactgaccct actgcatttg ttattgttac agatgtacat gaggttcttg
     1081 gagaaggatt taaaaattta gagaac
//
LOCUS       DACULQ010000343         1089 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.345, whole genome shotgun sequence.
ACCESSION   DACULQ010000343 DACULQ010000000
VERSION     DACULQ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.345"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            191..1060
                     /locus_tag="JA154_18805"
     CDS             191..1060
                     /locus_tag="JA154_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT7997829.1"
                     /translation="MDYLKYFKKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT          444 a           92 c          184 g          369 t
ORIGIN      
        1 gaaaaagcac catctgactt ttttgatttc attgaataaa aaatgtattt aattattcta
       61 aataattagg atatataaat caactaaaag tttttattta tcgtattgaa ttagttataa
      121 tattttagta tagtaaaaaa tagtgctatt ttacatattt aaagagttta agtagaagag
      181 aggtgccata ttggattact taaagtattt taaaaaagct gtaatgtata ttgagaataa
      241 tttacatgaa aatataactg taaatgatgt ggcaaaagaa actggatatt cttattatca
      301 tttaacacgc ttatttaagt ctatgtttgg tgagagtgta ggaagttata taaaaaaaag
      361 acgattagta agtagtacaa aagaactttt atatagtgat aaaaaagtga tagatatagc
      421 tattggtagt ggatttgaat cttcggaagc atttagcaga gcatttaaat ctatatacaa
      481 ggttagccct atagaatata gaaaaaatag aattgatgta tttgttggta aaaagaaaaa
      541 attagagtta gattttatga gacatcttgt tggaaatata acgattaaac cagttataaa
      601 agaaatagaa aaaataaagg taataggtat aaaggataaa gtaattttag aaaataattc
      661 attacctgac ttgtgggaga aattcagaaa agtacatcat attgtcccaa atactttgcc
      721 atcaaagaga gtttttggaa tctgtgaagc aatctctgaa atacacctag ttagtgaaag
      781 tatggaattt aatgagataa taggacttga agttaatagt tatgatgtga ttccaaattc
      841 atttgtttca aaaactatta aggaaggaaa gtatgctata tttactcata ctggtagctt
      901 agataattta gacaaaactt atgaatatat atggggtact tggtttttaa gttcaaaaga
      961 agaacttgat ataagagatg attttgaagt atatgatgaa cgatttttag gtcctagtaa
     1021 tgtgaactca caaattgata tttatatacc tataaaataa tctataagaa aactaaaaaa
     1081 taatttgat
//
LOCUS       DACULQ010000344         1081 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.346, whole genome shotgun sequence.
ACCESSION   DACULQ010000344 DACULQ010000000
VERSION     DACULQ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.346"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            186..455
                     /locus_tag="JA154_18810"
     CDS             186..455
                     /locus_tag="JA154_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997830.1"
                     /translation="MRGITMENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQ
                     NGGIMDTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            500..823
                     /locus_tag="JA154_18815"
     CDS             500..823
                     /locus_tag="JA154_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997831.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT          450 a           82 c          168 g          381 t
ORIGIN      
        1 taatatatta ttagtaatgg tacagaatac acatttataa tttatagatg tgtatttttt
       61 gtttaaaaaa tttaaatata taaaactata aaggttacaa ttttggaaat agatgtaaat
      121 atatgttgta ttgtataata agagtaatat aaaatctagt aaaaataatc aaataaattt
      181 agcttatgag agggataact atggaaaata aggattttaa attaacaata tgtatatttg
      241 cttttgtaat tggagtagta gtattattct gttgtatagg tttaggtgaa tttagtgcag
      301 agcatattat tcaacaaaat ggtggaatca tggatactga ccaatataat atttatctag
      361 accaatctat ttctcaatat agaaattttg gttctatatt agctctgtta ggaggaatag
      421 ggatattgtt tgataaatat atcagaaaat actagagatt atgttttgtc taaaataaat
      481 tcttatgagg agataaaaaa tggcaacaga aatatttact gtaaaaatta cacccttaga
      541 ggcacaaaaa atgataaaag aaaatcttga tgcagattta gtttttagtg attcttataa
      601 tttaggagat gataaattta tactaattac aacttttgag aagtattatg caagaaataa
      661 tagtgatgca ggattaatag tagtttatga aaataccaca ggaaaaacag ttattaaagc
      721 tacttcaaca ggcagtgcaa ttggaatttt aaaaattgat tggggtgcag gtggaaattt
      781 acttaaaaga gtaaaaaaca tacttgctga ttatattatt taattatttt atctaactga
      841 taatatgtca agaaaatctc gtaaaagagg ttaagaacat ttttataata tatagataaa
      901 taagaatttg aaggtaaagt ttttaaggta taaagaaaaa taatataata aaacgaattg
      961 gctttataat tctttataag gcaaagagca catttcttta tataattgtt ggatattaaa
     1021 taatatgtaa atacaccatt tacatataaa aatttaatta atatgttaag agaaaatgaa
     1081 a
//
LOCUS       DACULQ010000345         1066 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.347, whole genome shotgun sequence.
ACCESSION   DACULQ010000345 DACULQ010000000
VERSION     DACULQ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.347"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..786)
                     /locus_tag="JA154_18820"
     CDS             complement(<1..786)
                     /locus_tag="JA154_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997832.1"
                     /translation="MMKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNY
                     IIENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTELWFMFGIIACICYAFFIWYRD
                     FNGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGN
                     VTNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLIS
                     FLFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISN"
     gene            complement(792..>1066)
                     /locus_tag="JA154_18825"
     CDS             complement(792..>1066)
                     /locus_tag="JA154_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997833.1"
                     /translation="ITELNLLTYNILDKSLDEDKYVLDNREKRGIRSIEIDSNKIVAY
                     TSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNSMLEFEIK"
BASE COUNT          411 a          149 c           89 g          417 t
ORIGIN      
        1 attagatata agactacata caaatatgtt tatacttgaa tatagaacag taaatacatc
       61 actgtattcg taaggtataa taaattctat agaaattata aacactaaaa tttctataac
      121 taaaaataga aaacttatta aagtgcttac tttataaata gatttattta taagaattga
      181 tgtaaatact acagatacaa atcctatcaa taaaaatata tactctacaa atagtatttc
      241 aaagctatat ggaagtaaca tttttaactc atatattgtt tcttgtacaa aaccatagtt
      301 tgtgacatta ccaagcatat gtctaggtaa caacttactt gcaataaaca aagatattgt
      361 aagtactact gtatagctat acactagaaa taatatattt aataatttag atatataaat
      421 attgattcta tttttaggta gcattataag tgtatatata gatttacttc taccattaaa
      481 gtctctatac caaataaaaa aagcatagca aatacatgca attataccaa acataaacca
      541 caattctgta gaacccatta tatttctaaa gcttgagaaa cctgtaacat ttaccgtttc
      601 tatcgtattt ccgatttttg ttaactgttc acttttaact gcattttcta ttatgtaatt
      661 atagtcattt aaatttttta ctatgtttaa aattacaaaa gaacaacaac aaattattat
      721 aaatatataa ttctttatat ttctttttaa ttcaaattcg aataatctta ttaaatcttt
      781 catcaactca cctactttat ttcaaattct aacatactat ttgaactgtt aaattgtaat
      841 tctccttcat atacaatttc cttagaattt ttatttatta tatatagcat aaatttatca
      901 tcccaagata atatagagtt tagccttgat gtatatgcaa cgattttatt tgaatcaatt
      961 tcaatacttc taattcctct tttttctcta ttatctaaaa catatttatc ttcatctaag
     1021 cttttatcta atatattata ggtaagtaaa tttaactctg taattg
//
LOCUS       DACULQ010000346         1030 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.348, whole genome shotgun sequence.
ACCESSION   DACULQ010000346 DACULQ010000000
VERSION     DACULQ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.348"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..364
                     /locus_tag="JA154_18830"
     CDS             <1..364
                     /locus_tag="JA154_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAT7997834.1"
                     /translation="LYDFSSDKVIALIARILYFSDYFIVILFLIFIINSTKYSPFVLE
                     NVKRFKVMGFCLLINTIIECINGYNLNTNNIRIFGTDSGGISPLMVISFIFALMCFVI
                     AETFDKAIKIKEDNDLTI"
     gene            375..590
                     /locus_tag="JA154_18835"
     CDS             375..590
                     /locus_tag="JA154_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT7997835.1"
                     /translation="MAIVVNLDVMMAKRKISLKELAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
BASE COUNT          381 a          123 c          130 g          396 t
ORIGIN      
        1 tttatatgat tttagttctg acaaagtaat tgctttaatt gcaagaatac tatatttttc
       61 tgactacttt atagtcatct tattcttgat atttataatt aatagtacta agtattcccc
      121 attcgtactt gaaaatgtaa aaaggtttaa agtaatggga ttttgtttat tgataaacac
      181 tataattgaa tgtatcaatg gttataattt aaatactaat aatataagaa tttttggaac
      241 tgatagtggc ggtatctcac cacttatggt tatctctttt atatttgcac taatgtgctt
      301 tgtaattgca gaaacatttg ataaagctat aaaaataaaa gaagataatg atttaacgat
      361 atagaggtga tgttatggca atagtagtta acctagatgt aatgatggca aaaagaaaaa
      421 tatcacttaa agaattggca gaaaaaatag atattacaaa tgcaaattta tcaattttaa
      481 aaactggaaa agcaaaagct attagattta ccactctaaa cgaaatttgc aaagctttag
      541 actgtcagcc aggtgatata cttgaatatg tagaagatga aaatgattaa aaaagagtgt
      601 ttagaaacac tcttttttat tatactatat gatttttatt cttttactat attattgaca
      661 ttaatattaa ctttgtttaa agtcataaca aaatatgttc taagaaagaa actgttcctt
      721 tatctatttg ttatccatta cctataagaa ctgtatcaca ctaaataata cttttaaatt
      781 gataatttta ccacagattc ctatatacat tcttttctac ataatattta tttaaactgt
      841 tagcaaaaca tggaactttt tcatttactt atacctccat ttatattatt gcatagctta
      901 tagctatgtg ttataaatta cattattgat gattttatct aacatttgta atcttttaga
      961 tactctttga agttacaaca ctgttatact ctaattaagt aaactaaatc tgatacaagt
     1021 tggtgattat
//
LOCUS       DACULQ010000347         1026 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.349, whole genome shotgun sequence.
ACCESSION   DACULQ010000347 DACULQ010000000
VERSION     DACULQ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.349"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..196
                     /locus_tag="JA154_18840"
     CDS             <1..196
                     /locus_tag="JA154_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997836.1"
                     /translation="GSQVYAVFESKIDTVKIGDYENNTRAIQVLDNIQNFIENGTKYD
                     YITSNKVRYNVNKIFQMPAK"
     gene            complement(306..986)
                     /locus_tag="JA154_18845"
     CDS             complement(306..986)
                     /locus_tag="JA154_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAT7997837.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          406 a          137 c          117 g          366 t
ORIGIN      
        1 tggtagtcaa gtttatgcag tatttgaaag taaaattgat actgtaaaaa taggagatta
       61 tgaaaataat acaagagcta tacaagtgtt ggacaatata caaaacttca ttgaaaatgg
      121 aacaaaatat gattatataa catcaaataa agtcagatac aatgttaata aaatatttca
      181 gatgccagct aaatagtgag tgtgcatttt aatttaataa ctaaagccca ctttcttttg
      241 tggtatgaaa gagggcttta catatttgta aatatcgaag tacacttgtt ttatcaataa
      301 attatttaat aaggtataat ggaccaatta aacaataaaa attctcctaa aacagcaagt
      361 attaaatata taatagcttc atttttgtaa agagaggtaa ttttttcgct tatgtcatca
      421 tcaattgtaa tagcaatttc atcttgaata atatttttat atatagatgg gaaaatatct
      481 tttttcttta taagattaaa taaattattt acacctaccc atataaaata tcctaaaaca
      541 gcacctaatg tgttcatcat taaatcatca atatctgtag ctcttaagtt aaacaattga
      601 caaagttcaa ttgctaatga aaaacctaat ccagttaatg aaacattgat aatatttcta
      661 aaatttttcc aaattaaagg tagtaaaaat cctaaaggca taaacataat tatatttaaa
      721 atatatgtta agatgccaac tgagtcaaaa ggtattagat ttatttcatc aattctaaaa
      781 agcccactat accttcctat atcccatatg ctaccaattc cagctttact aagtgctaga
      841 tagatatata ataaaaaaat atatacccat actaaatgta tcattacatt cttttcaact
      901 ttcaaatttt ttcgttttat aacgactatt tggtatatta agcatggtaa tataacgcaa
      961 ataaatgaat aaaatgtcca tatcatttta ttttgtcccc tttcaaattt atgtttttag
     1021 ttttat
//
LOCUS       DACULQ010000348         1025 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.350, whole genome shotgun sequence.
ACCESSION   DACULQ010000348 DACULQ010000000
VERSION     DACULQ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.350"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            144..1019
                     /locus_tag="JA154_18850"
                     /pseudo
     CDS             144..1019
                     /locus_tag="JA154_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087478.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
BASE COUNT          417 a           91 c          165 g          352 t
ORIGIN      
        1 atcttaagct atcttattta attctaatac aggaagaagg taaatgtata ttgaaaaaat
       61 catagaaata gaagtaaaca atttaaataa atacaaaggt ttgcaaaaga agtaatataa
      121 ttatatacaa tgaggatgtg agtatgatga acagactaaa gccaagaatt ttaggtatta
      181 gtatcttgtt tattattata attattttaa catgtggagc tgttttcaag tttaaattta
      241 aattagataa taaattaaaa gaaaccacac atacatattt gtctgagttt acagaacaca
      301 atataattaa tctcaagaca agacttagtg gtcaatttga catgcttgaa tctattgcta
      361 acttcatagg aaacttgaat aatataaatg atgaaatggt gattgattta atggattctg
      421 atgtaaagcg aagttcttta attagaatga ctatctctac attagatggg gaatgttatt
      481 ctaatgatgg aattacccat aatgttcaag atagagatta ttttaaaaaa gctaaaaatg
      541 gagaaaagaa tatatcagag cctttaaaat ctattgttaa taataaagaa ataatagttt
      601 tatctacccc agtttataaa agtgggaaaa tagttggtgt tttaagtgga atttatgatt
      661 caaaaaagtt aaatgaatta ttaggattat ctgcatttga tgaaaaagca gatgtttttg
      721 ttgcaaagaa aaatggagat ataattgctc aaaaaacaaa taaaaatagt aagacgatat
      781 catgtatatt tcagcttttt gacaaagata attgtgatga agttgattgt ctaaaaacta
      841 taaaagaaaa tatgaaaaat gataagtctg agtattttaa ttataactta gataatgaaa
      901 aatttatgtt aaactatcag ccacttggag ttaatgattg gtatgttttt tctgttgtgc
      961 caaataaaat agtgtctatt cagtcagaga atatatctta tgatgtatat atttttgcat
     1021 gaaat
//
LOCUS       DACULQ010000349         1024 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.351, whole genome shotgun sequence.
ACCESSION   DACULQ010000349 DACULQ010000000
VERSION     DACULQ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.351"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..846
                     /locus_tag="JA154_18855"
     CDS             <1..846
                     /locus_tag="JA154_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT7997838.1"
                     /translation="ICKNYINPSIGKYKIQDLHPVHIQNYIDKLSYKLNPQSIKIHIN
                     ILKLAIKRAYRLKLIKENVMDSIEAPRYKKFKNEIYDREQMIKLLDLAKNTEMELPIN
                     LAIGLGLRISEVLGLTWDNIDFEENTITVNKITSRINGSVILKEPKTESSVRKISSPK
                     ELMSLLKEYKIKQNKNLLKSSIRNNNNLLFFNKKCEPIAEDVMSKKFKRFLEKNELPH
                     IRFHDLRHSHVTLLINSKVPIKVISERVGHSNISTTLSVYSHVLKEMDKEASDKISES
                     LFNAN"
BASE COUNT          428 a          121 c          124 g          351 t
ORIGIN      
        1 atttgtaaga attacattaa tccatctatt ggaaaatata aaatacaaga cttacatccg
       61 gttcacatac aaaactacat agataaacta tcttataaac taaatcctca atcaattaag
      121 attcatataa atatattgaa acttgcaata aaaagagctt atagactcaa attaataaaa
      181 gaaaatgtta tggatagtat agaggctcca agatataaaa aatttaaaaa tgagatttat
      241 gatagagaac aaatgataaa attattagat ttagcaaaaa atacagaaat ggaattacct
      301 attaatttgg ctattggatt aggtcttaga atttcagaag ttttaggtct aacttgggat
      361 aatattgatt ttgaagaaaa tacaataaca gtaaataaga taacttcccg aattaatggt
      421 tctgttattc tcaaagagcc aaagacagaa agctctgtta gaaaaatatc ttctcccaaa
      481 gaattaatgt ctttattaaa agaatataaa ataaaacaga ataaaaattt attaaaatct
      541 tccattagaa ataataataa tttgttgttt ttcaataaga aatgtgaacc tattgctgaa
      601 gatgttatga gtaaaaaatt taaaagattt ttagaaaaga atgaattacc tcatattaga
      661 ttccatgatt taagacactc acatgttact ttacttataa attctaaagt acctataaag
      721 gttatatccg agagagtagg acactcaaat ataagtacta ctctaagcgt ttattctcat
      781 gtacttaaag aaatggataa ggaggcttct gacaaaattt ctgaaagttt atttaatgct
      841 aattaatatt aatttatctt agctttaaat tagcttagct tattgagata gattcattaa
      901 tattaaatgt atctatctca aattaataac tacttaaata atctgtttta ttttaattta
      961 tcgcctcagt tattatactt ttttacgaaa aattatactt tacaataaaa aagatattcc
     1021 ctta
//
LOCUS       DACULQ010000350         1007 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.352, whole genome shotgun sequence.
ACCESSION   DACULQ010000350 DACULQ010000000
VERSION     DACULQ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.352"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            4..783
                     /locus_tag="JA154_18860"
     CDS             4..783
                     /locus_tag="JA154_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997839.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT          418 a          102 c          130 g          357 t
ORIGIN      
        1 gatatgaaag ttaatgaaaa taatataaat aatatagatg aagagttaga tgaacttagt
       61 aggaaagttt acttagagtc accagatttt tggataagta tttctgacaa aataatagat
      121 aaagtatttg atgaaatgtt attatttttt gcagttaaat ataactattt caatatagta
      181 tgctttgcta ttgaaaacaa ttatctggat ttaaacatgc cttcaaaaaa caaaaaatac
      241 actaatataa tggagcattt gttagataca tcaaaacaga atgaagataa aaaaatttat
      301 aattatcttc taagcttaaa taaaggtaat aacctagatg aaagaccaat tttagatgaa
      361 ttaaactgta atattagtag tgatactttt gaaaaaaaag atatatatct tccaaagtac
      421 ttatgtccaa gttgtaattc taatatattt gatacaggtt ataaagtatc aaatgacata
      481 acatataaat tttcttctaa agaagctgaa cctattgaaa tgtgtagtga agtttcttct
      541 atcagatgtt gtaattgtga tgaattatta gaagatatta cacttgataa actatataac
      601 ctatgcaaaa ttcaaaactg taataattgt ggttctaatt taactaaggt aggaattatt
      661 gcaaagaaaa aaatgaactt tgatgagaat actgaaaaat ttaaagatgt ctcaacatct
      721 tactgttgtg gaaattgtga ctctgaaata aacttatctc aaagagaaca ttttaagatt
      781 taaatattta taggctaaag taataagatt acattattag atactgtata agttttttaa
      841 atctgtaaaa ataaatacac ttataagaat taaaattgct atgaaactag ttcttgatga
      901 actaatttca tggcaatttt ctataatcta ataattatta aattttaaca aacctttatt
      961 agcattccat aattatttaa aattctttca aataccctat agtttat
//
LOCUS       DACULQ010000351          986 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.353, whole genome shotgun sequence.
ACCESSION   DACULQ010000351 DACULQ010000000
VERSION     DACULQ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.353"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(117..506)
                     /locus_tag="JA154_18865"
     CDS             complement(117..506)
                     /locus_tag="JA154_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT7997840.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          325 a          166 c           76 g          419 t
ORIGIN      
        1 acattatagt atcataatcc acttataatc atttgtcata ttacttaagt agactaatat
       61 attacataaa tacacttaca tacactttat attgatgcta taagaaatac gtttgcttat
      121 acgtctttct cctcttcttt tagatttttt atccactctt caataagtaa gatatcatct
      181 ttgctcaaat taatattttc atgtaatgac aacaataaac tttttataga agaatcatga
      241 atatttctaa aaaaatctct tgtctcaact cctatgtatt ccttttcttt aattaaaact
      301 tcataatgcg tatatttatc tatcttttga gaatttagaa atctttttac tactaatcta
      361 gataaaagag tcaaagttgt cgtttgtttc cattgatatt tttgctccat tcctaaaaca
      421 atatctctag atgttacttt ggaatctgat ttccatataa atttcattat ctttaattct
      481 gattgtggca acttttttat agtcatgata aaaccttgct actcagaata tagacaagca
      541 atatccataa taaaattttt cgattaaata catcttatac ggatttatat aagctcagac
      601 ttataagata tttttcaatc ttaatcctaa atttagctat ttgtacacgg taaatatata
      661 taaataatgt caaaattctc ttgtattata ttgcttaatt ttatgaaata ttttgtactc
      721 agctatctat tttggatact tgtccagtag ctctcctttc ttttaaactc tttgatttta
      781 aatatttgta tttcagtaaa tcctacttcc taaatttttt atttactaca acttattact
      841 gaacttccta cttcctaaac tttttattta ctacaactta ttactgaact tctcacttac
      901 taaatttctt acttactata aattataact aaacttctca cttactaaat ttcttactta
      961 ctataaatta ctactaaact tcctac
//
LOCUS       DACULQ010000352          981 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.354, whole genome shotgun sequence.
ACCESSION   DACULQ010000352 DACULQ010000000
VERSION     DACULQ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.354"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>981
                     /locus_tag="JA154_18870"
     CDS             <1..>981
                     /locus_tag="JA154_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7997841.1"
                     /translation="MYNKKTINKKLAIVFLISIVVQAIIQYLVLIFFMYIYYAEAIAI
                     VILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVIGDDEISTLAK
                     SINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDFDRETI
                     NDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFEDKIQN
                     SSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEENIILT
                     FKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHM"
BASE COUNT          418 a          101 c          138 g          324 t
ORIGIN      
        1 gtatgtataa taaaaaaact ataaataaaa aattagctat agtattttta atatctatag
       61 ttgtacaagc tattatccaa tatcttgttt tgatattctt tatgtatata tattatgctg
      121 aggcaatagc tatagtaatt ttatttacac ttgtaaacac agtttttcat ttttatatat
      181 acaacttttt atctgttaag tttaattaca taaataacat atcaaaaaat ataaaaaata
      241 tagaaaatgg caatttaaag tataaattag aagttatagg agatgatgaa atatctactt
      301 tagccaaaag tataaataat ataacaggcg gtcttgaaac tgctgtagat gctaatataa
      361 aaaatgaaag gatgaaggca gagcttatca caaacgtatc tcatgactta aaaactccac
      421 ttacatctat attaagctat gttgatatgt taaaaaataa tgactttgac agagagacta
      481 taaatgacta tataaatatt ttagataaaa aatcacaaag attaaaatta ttaatagatg
      541 acatatttga agcatctaag ctaagtagtg gagatgtaga atttaatata acaaaaaccg
      601 atataagaga acttttaatt caaagtattg tagaatttga agacaaaatt caaaatagca
      661 gtttagattt tataatagaa acacctgaat atgctgtttt taccatgata gatggtaaaa
      721 aaacttggag agtttttgag aatctaattt gcaatatact aaaatactct atgcctaaca
      781 ctagggtcta tatagatatg tttataaagg aagaaaatat aatattaacg tttaaaaata
      841 tttcaaatga taaactaaat ctaaaaccag aagagttaat agaaagattt agaaggggtg
      901 atatatctag aaaaacagat ggctctggtc ttggtttatc aattgctcaa aatataattg
      961 aattagaaaa tgcacacatg g
//
LOCUS       DACULQ010000353          979 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.355, whole genome shotgun sequence.
ACCESSION   DACULQ010000353 DACULQ010000000
VERSION     DACULQ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.355"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            270..737
                     /locus_tag="JA154_18875"
     CDS             270..737
                     /locus_tag="JA154_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAT7997842.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDYFDTFVERLSTMRDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          371 a           64 c          170 g          374 t
ORIGIN      
        1 taaaaaaaca tagatatttc agtaatctaa aatagtctac aaaaataaca gttttagcaa
       61 aattaataag aatacatata gatttaaacc taaaagtatg aatgtgtctg ttttttacaa
      121 gattaaaatt tatattatat gatacaatta acttaactat ttcaataaga cttataattt
      181 ttttctttat actagacctt attgaaacct ttatagttta attctaatta tatattataa
      241 ataaagtatg tgtatattaa ggaggtaata tgaagttttt aaatgtttta atagtagttg
      301 aagatattga gaaatcaaaa aagttttatt atgatgtttt ggggttaaag gtaatctgtg
      361 attttggaga aaatgttgta ttagaaggga atatatcatt acaagaaaaa aagctttggt
      421 tagagtttat taataagagt gacagtgaag ttaagttcaa tggaaatgat gctgagctgt
      481 attttgagga agattatttt gatacttttg ttgaaagatt gagtacaatg agagatatag
      541 attatgttca tctagctata gagcataggt ggggtcaaag agcaattaga ttttatgatt
      601 tagatgggca tattattgag gttggagaaa ctatgtcatc tgtgtgtaga cgttttttag
      661 atagtggtct ttcaatagat gaggtagcta aaagaatgga tgtaactgtc gaatatattg
      721 aatcagtttt agaatagatg gattatatta aagtaaaact atattttgta tagaagtaag
      781 ggctgtaagt aaacttgtat ggaaacaaat ttacttacag tttttgtttt tgtaagatac
      841 agaatataat atttgttata attttatttg gatttttaaa aagtgcatga ttttccttac
      901 atattataat agaatttaat atttattgga tgatttaaat ttagaaaaat ataagtatta
      961 gcttataaaa gataattat
//
LOCUS       DACULQ010000354          966 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.356, whole genome shotgun sequence.
ACCESSION   DACULQ010000354 DACULQ010000000
VERSION     DACULQ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.356"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            80..784
                     /locus_tag="JA154_18880"
     CDS             80..784
                     /locus_tag="JA154_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT7997843.1"
                     /translation="MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTII
                     LDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEEN
                     NKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
                     KCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKECKLLTDETYQ
                     EKIAWAIYIGIQKYLS"
BASE COUNT          423 a          102 c          152 g          289 t
ORIGIN      
        1 attaaaaaaa tttaaatgtt tgtactaata tatggaatat tatagtgttt ttataagtat
       61 acatatacag ggtgatgttg tgagaaagta cataaaacat ataattttta gttttgcaat
      121 gatatgtcta gtagtagtat ccatatttga aattaaaaat atttctgaag atgttatcaa
      181 gtatatgcca gtaacaaaca aaactataat tttagatgct ggtcatggag gcattgatcc
      241 aggtgcatta aataaggata agagtacatc tgaaaaggat attaacctag caataacact
      301 taagcttaga gagcttatag aatcaagtgg tggccttgta atattaactc gagaagatga
      361 cagtagcctt tataaagaag aaaataataa aactacaaga caaaaatata atgagaattt
      421 gaaaaataga aaagaaataa tatctaattc taatgccaat atgtttgtct ctatacatct
      481 taatgcattt gaacaatcaa aatactatgg agctcaaact ttttatccta aagacaaaca
      541 agacagtaaa gaattatcga agtgtattca agaagaactt aaaagagtag tagataagac
      601 aaacaacaga gaagtcaaac caagggatga tatatatctt ttgaaagaaa ataatattcc
      661 atcagtactg atagaatgtg gttttttatc aaatgaaaaa gagtgtaaac tcttaactga
      721 tgaaacatat caagaaaaaa tagcatgggc aatctacata ggaatacaaa aatatttaag
      781 ttgaaataac tataaaaata ggtctaaata tgtaaatagc ttgtattaaa gcagttaaaa
      841 tgcattaata taggctattt ttattttgac aaaaaaatat ttaaaataaa agttaaaaag
      901 ttgttgactt agaataatat agatgatatt atatatgagt gcccaaaagg agcaccaaaa
      961 taagac
//
LOCUS       DACULQ010000355          934 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.358, whole genome shotgun sequence.
ACCESSION   DACULQ010000355 DACULQ010000000
VERSION     DACULQ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.358"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            200..838
                     /locus_tag="JA154_18885"
     CDS             200..838
                     /locus_tag="JA154_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HAT7997844.1"
                     /translation="MPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNP
                     FIYIMDKHIDLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQ
                     SDIKKRVMNMFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVS
                     SIRFSVIGFTYADAPKNMREEYKINCESLGLIPKDSDKIEVGIEYYDSLLND"
BASE COUNT          376 a           84 c          134 g          340 t
ORIGIN      
        1 agtagtgtat ttaatttaca ctatatttaa atatataaaa cttaaaaatt taataaatga
       61 cttatcttat gatgttgatg atagagagat tgtaaatctt tataaaaata tattagaaga
      121 atttaatata acaaaagata taaaactcaa atactcttat gaagttgaaa caccagcatt
      181 ttttaattca tgtgtacttt tgccaccaca tgagtataaa ttgaaagagt tagactggat
      241 ttttagacat gagcttatgc attttaagag tagggacctt tatttaaaat atgtaatcct
      301 atttttaaaa acagtatatt ggtttaatcc atttatatat ataatggata aacatataga
      361 tttagattgt gaactttatt gtgatgaaag agttttaaaa aatcgaaatt cagaggaaaa
      421 aaaggattat gctttaacaa ttctaaatgc aatgagaaaa ggttcaaata cttcaaataa
      481 atttatagct ggacttcata agcaatctga tattaaaaag agagttatga atatgtttaa
      541 tgaaaagtac aagaatggta tattaatggc attaacttta tgtttattat cttctattac
      601 ttttttgaag gttaattcaa ctagtattgt taatcttaat gtattaccta aaggatttga
      661 agacttacga actgttagca gtatacgttt ttcagttata ggatttacat atgcagatgc
      721 tcctaaaaat atgagggaag aatataaaat aaattgtgaa tcattaggtt tgatacctaa
      781 agactctgat aaaatagaag ttggtataga atattatgac agtctattga atgattaaat
      841 aaatattatt ttaacagata ttaattataa agttttgaat agtaatttgt tgagaaataa
      901 atatatcctt ataaaaatag atacttatat acca
//
LOCUS       DACULQ010000356          930 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.359, whole genome shotgun sequence.
ACCESSION   DACULQ010000356 DACULQ010000000
VERSION     DACULQ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.359"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..257
                     /locus_tag="JA154_18890"
     CDS             <1..257
                     /locus_tag="JA154_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997845.1"
                     /translation="VGDIDIDAILDYAGNKRNRNSSKKNTFKLKIPFSTFIQMPRKIE
                     NKDKINLKYLIQDITSSILDDNLFISVTAIISYENSSKIE"
     gene            276..>930
                     /locus_tag="JA154_18895"
     CDS             276..>930
                     /locus_tag="JA154_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997846.1"
                     /translation="MDAMRITKTTFNDVFSSDANESIIKVEKYVDKKCASVFEILTYT
                     IIVTNTNRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKK
                     IISFKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLN
                     ISNTLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSC
                     "
BASE COUNT          406 a           77 c          134 g          313 t
ORIGIN      
        1 tagttggtga tatagatatt gatgcaatat tagattatgc aggaaataaa agaaatagaa
       61 attctagtaa aaaaaataca tttaaattaa aaataccctt tagtacattt atacaaatgc
      121 ctagaaagat agaaaataaa gataaaatta atttaaagta tcttatacaa gatattactt
      181 catctatttt agatgataat ttatttatta gtgttactgc aattataagt tatgaaaata
      241 gttccaagat agagtaatct ttaaattata gaaaggtgga tgcaatgaga ataacgaaaa
      301 ctacttttaa tgatgtattt tcaagtgatg caaatgaaag tattataaaa gtagaaaaat
      361 atgttgataa aaaatgtgca tctgtatttg agattttgac ctacactatt attgttacaa
      421 atacaaatag gtacaaaact ggaaatatat ttttcaaaga ttatatttct aaatatatag
      481 agtttataaa taatactgta aaagttaatg gcattataaa acgtggatta gacccacagc
      541 aaggttttta catagggaga atagatgcaa gttgtaaaaa aataatatct tttaagagtg
      601 tagttttacc aaattctgca cataggatta taaagaatag tgctaatata tactattatt
      661 ataagtgtaa tctagataaa ttcccaacaa gaatatcaat agaaagtaat agagtttata
      721 ctaatgtaaa taaaattgta ttcaaacaat tgaatataag taatacttta aaagctccta
      781 aaaatctaag agatatatta aaagtaaatg taaatagtaa aatatttgat gttaaaccta
      841 tgaaaaatct tataaacaaa ggtagtaatt tgaaattatg taacttgata gtttttgggt
      901 gtatagaagt tgaaatagac tatagttgta
//
LOCUS       DACULQ010000357          911 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.360, whole genome shotgun sequence.
ACCESSION   DACULQ010000357 DACULQ010000000
VERSION     DACULQ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.360"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(182..790)
                     /locus_tag="JA154_18900"
     CDS             complement(182..790)
                     /locus_tag="JA154_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997847.1"
                     /translation="MGYIASDEGFPFHDIPLSYMEEMKNRRISFYVLHSPLDNYSDYS
                     TSVSFAKAMGLEIVKPFCKYDDKIEVGVICKTSLKSIEEIKELIKKSVGHDVKLYDYG
                     DSVLKDGLVAIAAGGGSYPFVAREVAELGINLYITGFTKPLKHFEPVLEFHQIAKDNL
                     INVIGATHYSTEKFACMGMVDYFKNLGLESEFLQGKYYLEDL"
BASE COUNT          329 a          145 c           99 g          338 t
ORIGIN      
        1 atatcgaaaa ataatttaaa agacatcaaa actaaatctt ctaatcagca attaaaagtc
       61 tattttctta gtttaaaaaa taaagataga ttttttcaaa acactggata aataggtaaa
      121 aaaataaaca gttagtaaaa taaattttta ctaactgttt atttttattg tttaatatgt
      181 cctataaatc ctctaagtaa tactttcctt gcaaaaattc tgattctaaa cctagatttt
      241 taaaataatc gaccatgccc atacacgcaa atttttctgt agaataatga gtagcaccta
      301 taacattaat taaattatct tttgctattt gatggaactc taatactggt tcaaaatgtt
      361 ttaaaggctt agtaaaccca gtaatatata gatttatacc aagttcagca acttctcttg
      421 ctacaaatgg ataacttcct ccccctgctg caatagcaac taaaccatct tttaatacag
      481 aatctccata atcatacaat ttcacatcat gaccaacaga ttttttaatt aattccttaa
      541 tctcttctat tgattttagt gatgttttac atataactcc tacttcaatt ttatcgtcat
      601 atttacaaaa tggtttgact atttctaaac ccatagcttt tgcaaaacta acagaagttg
      661 aatagtcact atagttgtct agtggagaat gtaatacata aaaagaaatt ctacgatttt
      721 tcatctcttc catataagat agtggtatgt cgtgaaatgg aaaaccttca tctgatgcta
      781 tgtatcccat tgcatgatgt gaaaaaatta aaatatcttt ttcatcttta tctataactt
      841 gcttaattat ttctttatca ggaaaagtag ttgtgtatac tttattaata ttttgagcat
      901 tatctaatac t
//
LOCUS       DACULQ010000358          877 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.361, whole genome shotgun sequence.
ACCESSION   DACULQ010000358 DACULQ010000000
VERSION     DACULQ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.361"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(116..607)
                     /locus_tag="JA154_18905"
     CDS             complement(116..607)
                     /locus_tag="JA154_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 domain-containing protein"
                     /protein_id="HAT7997848.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT          313 a          138 c           81 g          345 t
ORIGIN      
        1 aatttaactt atatatttta aaaggctaat ataatatgta ttttcttcta tacaatcaaa
       61 aggcttcata tctaagatat gtgatgacac taatattcac aatttttagt aattatcaca
      121 ttataaaaaa tattacagct agtccatcat tagatgcttc ttgtataaac tttttaagct
      181 caattagatt tgcccataca tattcaaaaa aatcatcttc actctcatca ttcataacag
      241 gataaatatt attttctaac atctcattaa tattaaatct ttttctaaat gcttgttctt
      301 ccaaactgtt cattgctttt gatagttttt ttacatcatt tgctgtaatc aacatgacag
      361 gaaattctcc atcaatttcc ataagtatgt ttccacttag tactaatttg ctaaatatat
      421 tatcatcatc tccaccaaaa ggacaacctg tcaaagtaaa gtgaatggca tgccatgctt
      481 tatcaatatt gatagtacta ttttcgttgg catcttcaaa aacaaaatct tgtaaatttt
      541 cttctgcttg ctgtaattta aaaacattct cttcacttat tttagtataa catcctatca
      601 ttcccataat tacattcctc ctaagtagcg tttttttaat tgtaacttat aattaaactt
      661 agttacaata aaatatattg agaaaagtat tatttcatct taaatttaaa tacctatatt
      721 atcaactaaa attctgtgtt tatagcgtaa gtattatttt ttcctaaaaa ttaaatgaat
      781 cagaggttct ataggcaata ttgactataa ataagaacta ttacttttta aaaaaaattt
      841 aagaaattat actgtagaat catgctctac ttattag
//
LOCUS       DACULQ010000359          787 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.363, whole genome shotgun sequence.
ACCESSION   DACULQ010000359 DACULQ010000000
VERSION     DACULQ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.363"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            88..471
                     /locus_tag="JA154_18910"
     CDS             88..471
                     /locus_tag="JA154_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997849.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
BASE COUNT          334 a           75 c          118 g          260 t
ORIGIN      
        1 ataaactata ctttaaatat agatatttaa tttaaataaa ttttacagta ttaatagtaa
       61 attatcttag aaaggaatgt gctttatatg tctacagaca aagttgttgg aatcatagat
      121 gaagaaacgg cagaacttgc aggaattgaa tatactggta aaatttatgc ttcttctggt
      181 gttataaaac atataaagaa aaaacataga tgtcaactga gtaaggatat ttttaatgat
      241 ataatagaca ctataaaaat ggtattaaag tcacctgaat atataggaag tcatcctaaa
      301 aaacctggaa aaagtgtgga gtttataaaa aaaattgatg attatatttt agtagctaca
      361 gaattagata caaaaaaagg atatttatat gtatcttcaa tgtatgctat aaaagaagca
      421 aaattagaaa gtagagtaaa cagtggaaga gtaaaacaat atgatggtta acataatatt
      481 attaattttc cctaaaatat tgaaaaatat tattatataa gttataatat taatatacac
      541 gtagatattt attagttaga tttgaggttg gaaaaggcac ccaatacgct actatagagt
      601 agttaataga gatgcaggat acgccgccct gccaaatcta attaaattaa aaagagagga
      661 ttaattcctc tcttattgtt tttttgagat actaatttaa tctataatag aaagattgaa
      721 ggtcgtttat acaaaaaaat tttcagttat atacattaaa ctaattaaat aagatattta
      781 taatcta
//
LOCUS       DACULQ010000360          774 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.364, whole genome shotgun sequence.
ACCESSION   DACULQ010000360 DACULQ010000000
VERSION     DACULQ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.364"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(35..574)
                     /locus_tag="JA154_18915"
     CDS             complement(35..574)
                     /locus_tag="JA154_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAT7997850.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(576..>774)
                     /locus_tag="JA154_18920"
     CDS             complement(576..>774)
                     /locus_tag="JA154_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HAT7997851.1"
                     /translation="EKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
BASE COUNT          222 a          123 c           63 g          366 t
ORIGIN      
        1 aaattacata ctaaattatt gtgattttat atttttattc atttgttgcc acttcttgat
       61 aattttctat aggtggcaat tcttcaagac tttctatatt caacaacttt aaaaattcat
      121 ctgttgtttt ataaattatt ggtttcccta tcttattcaa tctaccagct tctcttataa
      181 gttcattttc aaacaaagtt tgtaatacct tatcacattt tactcctctt atatcctcta
      241 tttctacctt tgttattggt tgtttgtagg caattatagt aagagtttct aatgtagctt
      301 ggctcaaact tttctttttc tttggttcaa taatcttctt tatatattct gcatagtctt
      361 tatttgtaca catttggtac ttattctcta acttgattat ttgaatacct ctattttgtt
      421 ctctgtactc ctcaataagt aaatttaaca taatctctat ttcttttgat gaaagttctt
      481 tatttattat atagtttaat tcttttatac ttattggctc accatatgca aacataacag
      541 attctataat atactttata tcttctcttt tcattttaac actccgaact ttttcttatt
      601 agaatatcat caaaaaatat atcttgtact acgactattt ctttttcttt tataagctct
      661 aatatagata aaaaagtagc tattatttca tcattttcat atgattttat aatatttgta
      721 aaagtaactt caatcttctc ttttataata tctcttatat ataatatttt ttct
//
LOCUS       DACULQ010000361          765 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.365, whole genome shotgun sequence.
ACCESSION   DACULQ010000361 DACULQ010000000
VERSION     DACULQ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.365"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..503)
                     /locus_tag="JA154_18925"
     CDS             complement(<1..503)
                     /locus_tag="JA154_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAT7997852.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTIAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVK"
BASE COUNT          262 a          128 c           77 g          298 t
ORIGIN      
        1 tcttttacaa gaacttttat aatttctgaa atgtacatag tatgatagtc tttctctgga
       61 taccattttt tatcattttc tttagcatca aagttttctg gtggcatatt accatgatat
      121 agctttttac atactataat catattagct tcttcaaaag ctaccgtatc atccacaaaa
      181 tatggtgtaa gtcctgcttt ttctatttta tttatatctc taccagaaat agtgccacaa
      241 atatttaaag cttctctata cgtttcatca aagaaggcaa ttgtaaatgt atcattacta
      301 tctacaaatt cttttgtata tctctgtggt cttatgtaag ttgtaacaac atttttaccc
      361 caatagactc caacaccacc ccagctagca gtcattgtgt taaacttttc tgaatcacca
      421 gctgttataa gtaaccattc tttgccaatc ttagtaaatg gattaaactg taattcctca
      481 atttttactt ctctaaaacc catgacttca tcctaccctt cattttttaa ttatataaat
      541 taattgttaa aaatttgaat atatgtactc ttataatact gacttttgat tgtcctacat
      601 aattcatgta aattataata caacaaatca attactttcg caataagtag ttttcatatt
      661 ttataagtgt aaaatataga taaaatatat tgattataaa gtatataata tatttttaaa
      721 cctaaagcaa tatacaataa aaaaatgtag aaagtaagta tctct
//
LOCUS       DACULQ010000362          757 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.366, whole genome shotgun sequence.
ACCESSION   DACULQ010000362 DACULQ010000000
VERSION     DACULQ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.366"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            302..>757
                     /locus_tag="JA154_18930"
     CDS             302..>757
                     /locus_tag="JA154_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HAT7997853.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCE"
BASE COUNT          352 a           58 c          142 g          205 t
ORIGIN      
        1 ataaaagatt gatataagat actgaagagg tagaaacaaa agtattaata gaagatattg
       61 aaaaaataaa aacaaaagat tgatagaaag tactgaatag atagagataa aagtgttaat
      121 agaagatatt gaaaaaataa aagattgata taagatactg aagaggtaga aacaaaagtg
      181 ttaatagaag atactaaaaa agtaaaaata aaggattaat aaaaggtact gaagagatag
      241 agaaaaaaat gttaatagaa gatactgaaa aaatagaaat aaaaaattag gaggaaaatt
      301 tatgaaatat caggtttcat tattagcagt gaaggatatt gaagtatcaa aaaaatttta
      361 caaagaatta tttgagcaag aaattgaatt ggacttaggt aaaaatgtaa catttaaagg
      421 aggatttgct atacaagaag acttcgcatg gcttacaggg attaatccag atacaattat
      481 acaaaaatca aataatatgg aactttattt tgaggttgat gattttgacg aatttattga
      541 aaaactcaat tattataaag atgtagagtt tgtccataag ccactaaaac atgaatggca
      601 acaaagagta gtgcgtattt atgacccgga taagcatatt attgagatag gagagtctat
      661 ggaagttata gctagaagat atttatctga gggatactct gtagaagaaa cttctaaaat
      721 aattcaacat ccaattaaat ttgtaaagca gtgtgaa
//
LOCUS       DACULQ010000363          735 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.367, whole genome shotgun sequence.
ACCESSION   DACULQ010000363 DACULQ010000000
VERSION     DACULQ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.367"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            26..487
                     /gene="ribH"
                     /locus_tag="JA154_18935"
     CDS             26..487
                     /gene="ribH"
                     /locus_tag="JA154_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088200.1"
                     /note="RibE; 6,7-diimethyl-8-ribityllumazine synthase;
                     DMRL synthase; lumazine synthase; beta subunit of
                     riboflavin synthase; condenses
                     5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione
                     with L-3,4-dihydrohy-2-butanone-4-phosphate to generate
                     6,6-dimethyl-8-lumazine (DMRL); riboflavin synthase then
                     uses 2 molecules of DMRL to produce riboflavin (vitamin
                     B12); involved in the last steps of riboflavin
                     biosynthesis; forms a 60mer (icosahedral shell) in both
                     Bacillus subtilis and Escherichia coli; in Bacillus
                     subtilis this 60mer is associated with the riboflavin
                     synthase subunit (alpha) while in Escherichia coli it is
                     not; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAT7997854.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLD"
BASE COUNT          287 a           86 c          115 g          247 t
ORIGIN      
        1 ataaataaaa ttggaggaag taaaaatgat atatgaagga aaacttattg gaaaagattt
       61 aaaaataggt ataataaatt ctcgttttaa tgaatttata acatcaaaac ttttatcagg
      121 agctgaagac tgtcttttaa gacatgacgt ttctccagaa aatatagaaa tagtttgggt
      181 acctggagct tttgagattc ctcttgttgc tcaaaaaatg gctaaaagtg gtaagtatga
      241 tgctataatc tgtttaggct gtgttataag aggggctact tctcattatg attatgtatg
      301 tagtgaagtc tctaaaggga tagccaaagt atctttagac agtgaattac ctgttatatt
      361 tggaatcgta actacagaaa atattgaaca agctattgaa agagcaggta caaaagctgg
      421 aaataaaggt tatgattgtg ctatgaatgc gctagaaatg gcaaatttat ttaagtctct
      481 agattaaata atatcattaa taaatattaa aaattagtgt aaaggataca aaatattaag
      541 taccaaaggg agaaactatc tttcttcttt ttggtactta atatttttaa tttaatcata
      601 tgtacgttct tatcaaacaa attgcatatc cttctaaaat tatacgttat ccatcaaatc
      661 tacttttaga agcttaaaat aattacaaat ataaccctaa acataaaata tcataaaaac
      721 atatatatta ctatt
//
LOCUS       DACULQ010000364          711 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.369, whole genome shotgun sequence.
ACCESSION   DACULQ010000364 DACULQ010000000
VERSION     DACULQ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.369"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..625
                     /locus_tag="JA154_18940"
     CDS             <1..625
                     /locus_tag="JA154_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HAT7997855.1"
                     /translation="LLLKELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDY
                     ISPIIFLTNHIEMILGIFDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYR
                     SNFFTIYSDFSLWKISFDEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFI
                     RVHKSFVVNKYHIVSLDLKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT          296 a           78 c           91 g          246 t
ORIGIN      
        1 tttgttatta aaagagctta ctgaaaaaaa taaagtaaga atatatttta tagttgtaga
       61 cgcaaaatat aagataagta atgaattatg tgatggatta tggatagcac aaaaaattag
      121 agaatcagat tatattagtc caatcatatt tcttacaaat catatagaaa tgattttagg
      181 gatcttcgat tataggttag aagttatgga ctttatacta aaacatgata tggaaattgc
      241 tgaaagtaaa ataaaagctt gtattaaaat tgctcacaaa agatacatta aagaaaaaaa
      301 ttatcgctct aattttttta ctatctattc agatttctca ctatggaaaa tttcatttga
      361 tgaagttata tattttgaaa caagcactat tccacataaa ataaaattag tgactacttc
      421 tagaatattt gaattctata aaagtttaaa gtctttacca gatttagatg catgctttat
      481 ccgtgtacat aaatcttttg tagttaataa atatcatatt gtttctctcg acctaaagaa
      541 aaataatatt aaaatgagta atggccatat atgtcgtata tctaatacat atagaaatat
      601 tttaaagaat attattaaaa cataataaat agaaaaagga gatgtctcaa gataaaaatt
      661 tgaaccatct cttttttata caaaaagagt aatccctaag aatcctctat a
//
LOCUS       DACULQ010000365          692 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.370, whole genome shotgun sequence.
ACCESSION   DACULQ010000365 DACULQ010000000
VERSION     DACULQ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.370"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..585
                     /locus_tag="JA154_18945"
     CDS             <1..585
                     /locus_tag="JA154_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997856.1"
                     /translation="EGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFG
                     LVAFVNKNYSNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKN
                     GKDYKEWSYEGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLK
                     KISSSDTLSEEVKKEIKNWENNLNNLNMNKNNSN"
BASE COUNT          303 a           64 c          110 g          215 t
ORIGIN      
        1 gaaggtttaa gtatagaaca attaatagag attagagata aagccattga taaaaattta
       61 gaaggtctta gggaatttgg agaagattca gtaaaaaaac gtaaagattc aattttaaac
      121 tttgaatttg gattagtagc ttttgttaac aagaattata gcaacttaac agaaaaagag
      181 aaattaataa gacaaattaa tattgttaac tatgaaaact ctataaatga tataatggat
      241 ccttcaggtt cattctttga attagcaaat cagaattacc aagtgaatga gtttctatta
      301 aaaaacggaa aagattataa agaatggtca tatgaaggtc tagattttct aattgatcat
      361 ctagaagaac tgtgcaaatt tgttgatttt gatgtaaaag atgtaataga tattattgat
      421 cctacaataa aaacagacct ttcagaggaa caaaagaaat ctttgaatga taaattaaag
      481 aaaatatctt caagtgatac tttaagtgaa gaagttaaaa aggagataaa aaactgggaa
      541 aacaatttaa ataatttaaa tatgaataaa aataattcta attaattttc tcataagaaa
      601 aatgcttata tcgtgataat gtgaaatgta cataattata aacaaaaaag gggctgtgac
      661 actaaaaaat agtgctacgc ccttttttat tt
//
LOCUS       DACULQ010000366          668 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.371, whole genome shotgun sequence.
ACCESSION   DACULQ010000366 DACULQ010000000
VERSION     DACULQ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.371"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            11..>668
                     /locus_tag="JA154_18950"
     CDS             11..>668
                     /locus_tag="JA154_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997857.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     N"
BASE COUNT          278 a           74 c          101 g          215 t
ORIGIN      
        1 tcgagtaatt ttgaaaggaa acatagtaca atataatttt gcagatatag aggaagaagt
       61 atattcgttg gattatgcta tagcatggaa tacagacgag gaaaacgtaa acataatacc
      121 atttacaaat aaattctgta aagaatctat agaatctttt tgcctaggta aaattaataa
      181 ctttgttgaa atattaaatg aaggttttgt tgaaaatcat cattatgtac atttagataa
      241 aatgattagt gttcctaaga aaaaggttaa tttagtatat caacaagaca cacatggtta
      301 tcttctaaga gatgataatg acaaccttat tcctgcaaaa ataacttcag agcaatctaa
      361 atctatatca agtaagatgg agttattctg tgctggtgaa gaaaaatgtt taattaatat
      421 attactaaag gctgacccat catatatttt agatgtagat agtataaaag acaaaaacat
      481 tctaaatcta ggttatgagt ctatcgacag atataaagaa tacaattttg atgatgataa
      541 gattttaata ttttttataa ataaaaaacg ttattcagtg attatgaaaa aaacaaacaa
      601 ctctgataac gatttagttt ccagaaacaa tgctattaaa gaattattta ctaataaagc
      661 aggaaatc
//
LOCUS       DACULQ010000367          636 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.372, whole genome shotgun sequence.
ACCESSION   DACULQ010000367 DACULQ010000000
VERSION     DACULQ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.372"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..635)
                     /locus_tag="JA154_18955"
     rRNA            complement(<1..635)
                     /locus_tag="JA154_18955"
                     /product="23S ribosomal RNA"
BASE COUNT          140 a          175 c          117 g          204 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gttatgatat gtccgcaacc ccaataaaga aaactttatt
      361 ggtttgggct aatccgcgtt cgctcgccgc tacttacgga atcgaatttc tttctcttcc
      421 ttcaggtact tagatgtttc agttccctga gttcccctca ttaagctatg tattcactta
      481 atgatactta gacattactc taagtgagtt tcctcattcg gaaatcttcg gatcaaagtt
      541 tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc cttcatcggc tcctagtgcc
      601 aaggcatccg ccctgcaccc ttaataactt gaccag
//
LOCUS       DACULQ010000368          587 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.373, whole genome shotgun sequence.
ACCESSION   DACULQ010000368 DACULQ010000000
VERSION     DACULQ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.373"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            87..572
                     /locus_tag="JA154_18960"
     CDS             87..572
                     /locus_tag="JA154_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAT7997858.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          263 a           44 c           99 g          181 t
ORIGIN      
        1 caaaaagata ataattcttt ttgttaaaag tatttttaga gttatatatt ttaataactt
       61 aatatttggc aagaggaggg cttaagatga tatctgaatt aatgttaaaa aattttcgca
      121 aatctagcat caattttaag actaaaatta aattgaattt aagagaaaaa ccagacataa
      181 atagtttgaa attaaaaagt ataccagaag gtaaaattgt taagttaaag tgtgtagatg
      241 gaatatgggc agaagttgag agtaattatg acaaaggttg gttactttat aaatatttag
      301 aaagattaag taatgctaaa aacaatagta atgatacaat aaaaaataga aaaaagaatt
      361 acataggaaa tataagaaca aatggattaa gtttagaact tagaaatgat agaacattag
      421 aatctaaagt tataactaca atccctgatg gttttaaagt tgaagtatgt tatagcgttg
      481 gaaaatgggc aagagttaat atcaataaaa atgaaaaacg atattctgga tatgtatata
      541 atcaatacat agaagagcat ttaagtttat aatacgatac tagcaag
//
LOCUS       DACULQ010000369          586 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.374, whole genome shotgun sequence.
ACCESSION   DACULQ010000369 DACULQ010000000
VERSION     DACULQ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.374"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>586
                     /locus_tag="JA154_18965"
     CDS             <1..>586
                     /locus_tag="JA154_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997859.1"
                     /translation="KISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELDHININ
                     FGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVGFEMNE
                     ESKDINFVIDEKKEDLEKGVFPSEDSDKEIEYEKLDMNNFTRTRYKVDKSTDDYYAKI
                     LSSKRYKKYLYVSVLYATSDSSENNKEYSSTIDII"
BASE COUNT          270 a           47 c           85 g          184 t
ORIGIN      
        1 taaaatatct atacttatta tatttttgac tttagcaata tattttagta taggtttatt
       61 aaataatgaa gataatttaa agataaaaac tataaaaggt gactctaatg aattagacca
      121 tataaacata aattttggat atgctttgaa tggatatata aattcatatt atacaataaa
      181 tggaagcaac aaaactaaaa agactgaatt aataaaacta caaaaggata gtaagaacac
      241 aaataattta attgatgtat ctagtggttc agatttaatt gctcatgttg gatttgaaat
      301 gaatgaggag tcaaaagata taaactttgt gatagatgag aaaaaagaag atttagaaaa
      361 aggtgtattt ccatcagaag atagtgataa agaaattgaa tatgaaaaat tagatatgaa
      421 taattttaca aggacaagat ataaagtaga taaaagtaca gatgattatt atgcaaagat
      481 actatcatca aaaagatata aaaaatactt atatgtatct gtactttatg ctacaagtga
      541 ttcatcagaa aataacaaag aatattcaag tactatagat ataata
//
LOCUS       DACULQ010000370          577 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.376, whole genome shotgun sequence.
ACCESSION   DACULQ010000370 DACULQ010000000
VERSION     DACULQ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.376"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>577
                     /locus_tag="JA154_18970"
     rRNA            <1..>577
                     /locus_tag="JA154_18970"
                     /product="16S ribosomal RNA"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DACULQ010000371          553 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.377, whole genome shotgun sequence.
ACCESSION   DACULQ010000371 DACULQ010000000
VERSION     DACULQ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.377"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            196..>553
                     /locus_tag="JA154_18975"
     CDS             196..>553
                     /locus_tag="JA154_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAT7997860.1"
                     /translation="MYNVIDLFAGAGGLSLGFEMTKKFNMVAFVEKNDNAAKTYLENH
                     PSVKRYCDIKRLDFQDILNSVDKIDVVIGGPPCQGFSNANRQKRKIINGNNELVKLYV
                     DAIDKLKPNVFVMENVK"
BASE COUNT          234 a           42 c           98 g          179 t
ORIGIN      
        1 taaaaaatat attatgttat ttatttgaat agattatcca gaagacataa ataagaaaaa
       61 taatgtgtaa gtaaaaatat tagaatttat taaaagctat tacaagtata agaaatttga
      121 aatgattatt caaaaattga catgtgaaaa aaaatatgat atgctgcaat aaaagttaat
      181 gtgaggtatg tcagaatgta taatgttata gatctttttg caggtgcagg tggtttgagc
      241 ttaggatttg aaatgactaa aaaatttaat atggtggctt ttgtagaaaa aaatgataac
      301 gcagcaaaaa cataccttga aaatcatccg agtgttaagc gttattgtga tattaaaaga
      361 ttagattttc aagatatatt aaacagtgtc gataagatag atgttgttat aggaggaccg
      421 ccatgtcaag gtttttctaa tgctaataga caaaaaagaa aaattataaa tggaaataat
      481 gaattagtta agctatatgt tgatgctatt gataaattaa agccaaatgt atttgtaatg
      541 gaaaatgtca aaa
//
LOCUS       DACULQ010000372          536 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.denovo.378, whole genome shotgun sequence.
ACCESSION   DACULQ010000372 DACULQ010000000
VERSION     DACULQ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.denovo.378"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(57..536)
                     /locus_tag="JA154_18980"
     CDS             complement(57..536)
                     /locus_tag="JA154_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAT7997861.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          215 a           93 c           45 g          183 t
ORIGIN      
        1 tactagttag tcactctttt cgggttattt atctcctata tattaaagtg tcacacctaa
       61 atagttaaat cattatcctc ttttatcttt attgccttta caaatccatc tgcaattaca
      121 aaactcatca gagaagttat aaaatataca aatactggaa cagtaaaata aaatccaaat
      181 cctaaatcta gaaatctcat tccttttcca gtagtaccaa aagttgaaat aaactcaatt
      241 agagagttta aaagcaaaag atatccaata atcctaaatc tttttacatt ctcttttaca
      301 aataggctat attcagaact acctaatact tctatcaatg attttataat gataaaatat
      361 gatacaccta aaataaacat taatattcca taaatagtac cttgtagagt tgcttctcca
      421 ctaaaacaga ggtacataaa acttttaaat cctcctacaa caattactaa tcctaaaaga
      481 aaaaataaaa tttgtaatat accacttagt aaatttttta taagtttatt agacat
//
LOCUS       DACULQ010000373        12774 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.guided.116, whole genome shotgun sequence.
ACCESSION   DACULQ010000373 DACULQ010000000
VERSION     DACULQ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12774
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.guided.116"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            187..804
                     /locus_tag="JA154_18985"
     CDS             187..804
                     /locus_tag="JA154_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT7997862.1"
                     /translation="MCKKMYLLVVGILIAGLLTACNGSPTKDSEQAQDNTKKEANVSN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDFNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            912..3443
                     /locus_tag="JA154_18990"
     CDS             912..3443
                     /locus_tag="JA154_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT7997863.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDEDEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3877..4578
                     /locus_tag="JA154_18995"
     CDS             3877..4578
                     /locus_tag="JA154_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanS"
                     /protein_id="HAT7997864.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4568..5710
                     /locus_tag="JA154_19000"
     CDS             4568..5710
                     /locus_tag="JA154_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HAT7997865.1"
                     /translation="MKSKNIKVNPDFTQVRNKIFMRTALMAFIAIIFIWILYSFILYG
                     KFSIWIVSFFENAFRMEYKAALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTK
                     YFSEINQGIDSLIKEDVGEVALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELI
                     VYLAHDLKTPLTSVIGYLTLLRDESQISEELRKKYLSISLDKAERLEDLINEFFEITR
                     FNLSNISLEYSTVSLTRMLEQLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVF
                     DNLLRNAVYYSFEDTTIEITAVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSR
                     NSNSGRAGLGLAIAKEIVELHNGTITAHSENNIITFKVIIPIVGNL"
     gene            5971..7071
                     /locus_tag="JA154_19005"
     CDS             5971..7071
                     /locus_tag="JA154_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase"
                     /protein_id="HAT7997866.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7068..7874
                     /locus_tag="JA154_19010"
     CDS             7068..7874
                     /locus_tag="JA154_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAT7997867.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7892..10030
                     /locus_tag="JA154_19015"
     CDS             7892..10030
                     /locus_tag="JA154_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid racemase"
                     /protein_id="HAT7997868.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10344..10964
                     /locus_tag="JA154_19020"
     CDS             10344..10964
                     /locus_tag="JA154_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAT7997869.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11130..11666)
                     /locus_tag="JA154_19025"
     CDS             complement(11130..11666)
                     /locus_tag="JA154_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAT7997870.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(11779..12483)
                     /locus_tag="JA154_19030"
     CDS             complement(11779..12483)
                     /locus_tag="JA154_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAT7997871.1"
                     /translation="MKKKILIPVIMSLFIISQCITSFAFTPENNKFKVKPLPYAYDAL
                     EPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVR
                     NNAGGAYNHKFFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWA
                     WLVATKDGKLSIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVV
                     NWNGALENYKNLKSQD"
BASE COUNT         4861 a         1368 c         2196 g         4349 t
ORIGIN      
        1 tattatttca acaataaaaa agaggtctgt ttctattttg agacaatatt atttttatgt
       61 agatttaatc ttaatgagta taaagtttta aaactactaa aataggagga attttaatat
      121 aaaagggtaa ataaagggtt tagagggcat aatatagaat aaattaatta tttaaggagg
      181 gtttttatgt gcaaaaaaat gtatttatta gtggtaggta tacttatagc aggtttatta
      241 actgcatgta atgggagtcc aactaaggat tcagaacaag cacaagataa tacaaaaaag
      301 gaggcaaatg tatcaaataa cgctttaaaa gatgaaaaaa ataatgaaaa tttaatggaa
      361 caagatttta aagtgccata cacagatgca ataaacatat ttaaagacaa atacaaagat
      421 gctgatatag tagatttaag tcttgagaga gatttaaata aatttgttta tactgtggaa
      481 ggtgtagatg ataataatga atataagatg aaaattgatg ctaatacaaa agatgtatta
      541 gaagataaaa cagaaaagtt ggattcagaa gatttaaatg gagtcgctag aaaagaaaaa
      601 ttagatttta atgacataat gacacctcaa caagctatgg aaattgcatt aaaagaacaa
      661 aatggaatgg ttaaagagtg gagtttagat aaagatttag atgttacttt ttataaaata
      721 agaatagata aagataaaaa tgaatatgac ataaaagtag attctaaaaa gggaactgta
      781 ttgaaggtag aaaaagaaga ttagaagatt taaactatct ttaaaataat ttcttttttg
      841 aataaaattt gtttaaaata taacaagtag ggtataaaga gactaaataa ctattttagg
      901 aggagtacgg tatgtcattg aaatcgctaa aaataaaaga aaatttatat tgggtaggtt
      961 cgcttgaccc tgatttaaga gtatttgaca taattatgta tacaccttat ggaactactt
     1021 ataattcata tgtattaaaa ggaacagaaa aaacagtttt atttgagaca gttaaagata
     1081 agcattttga caattatatt gaaagattaa atgatttaaa tatagatttt gaaaaaatag
     1141 actatattgt tgtaagtcat actgaaccag accatgcagg aagcgttgaa aagcttttgg
     1201 atttagctaa aaatgcaaaa gtagtagctt cagaaacagc aattaaatat cttaaggaaa
     1261 tcgtaaacaa agactttgaa tatgttgcag ttacagatgg agatacatta tcaattgggg
     1321 acaaaacttt agaatttttc tctgtaccta tgcttcattg gccagatacc atatacacat
     1381 atataaaaga ggataaaact cttgtaacat gtgattcatt tggtagtcac tatagtaatg
     1441 ataagatagt aaatacactt gatgaagatg aagaaaagga ttacttggat gctttgagat
     1501 actattatga ttgtataatg ggaccattta aaccatctat ggtaactgcc atagaaaaga
     1561 taaaagattt agatatagat actgtatgtc caggtcatgg tccagtatta actgaaaatc
     1621 ctagaaaaat tatagacctt tattacaact ggagtgtaaa tgaacaaata aagttagaaa
     1681 aagaagttac aatttgttat gtttctgcac atggatatac aaagattatg gcagaagcta
     1741 taaaagcata tattgaaaaa aatagcaact ataaagtaaa tttatttgat gtaatagagc
     1801 ataagcaaga agatattttg gcaaaaatag cagtatcaca aggtgtatta tttggaacac
     1861 caacaatatt aggagatgcg ttaaagccta tatgggatat attaatatcg ttaaacccag
     1921 ttcttcatgg tggaaaagtt gcttcagtat ttggttcata tggatggagt ggagaaggta
     1981 tagaaaatgc tatggagaga ataagtcagc ttagaatgac agcagtaaaa ccttttgctg
     2041 ttaactttaa accatcaaat gaagagattg ataaattaca ttcttataca ggtaaattcc
     2101 tagataagtt aaattctact tttggaagta aaaagaaaac taagaaattt aaatgtgtaa
     2161 tttgtaatga agtttttgaa ggtgatagtg ctcctagtgt atgccctgta tgtggagcta
     2221 aggaagacca atttatagaa gttgaagaag atgaagttac ttttagaaaa gatactgatg
     2281 aatatttcgt aatagtagga aatggagcag ctggttttta tgcagcagat gctataagaa
     2341 aaagaaataa aacttgtaag ataactatga tttctaatga agatgagttg acttactata
     2401 gacctgcact ttctgatggt ataaatgaag aacttggttc agatttctac atggaagata
     2461 aagattggta tgataaaaat aatatagtag taatattagg aacgaatgta gataaattag
     2521 atgaagtgaa taaaactatt atagtgaatg atggagctat taaatttgat aagcttgtaa
     2581 tagctacagg aagtagaaac tttataccac caattaaggg tcatgattta gaaaatgtgt
     2641 ttactcttag aaatataaaa gatttatata gtgtaaaaga agcactagaa aaatctaaga
     2701 aagttgtagt gattggtggc ggtttattgg gacttgaagc tgcatgggaa tttagactaa
     2761 aaggattaga agtagtagta gttgaagcca tggatagcat actatcaaaa caacttgata
     2821 aagaaggcag taaaatatta gaacaatgtg taagagatac aggaatagat gtaagattgg
     2881 gtgtagcagt tgatggtatt gaaggtgatg gaaaagctca aaaagttgta tttaaagatg
     2941 gagatagtgt tgactgtgat atggtagtat tttcaattgg tgtaagagca aacactcaaa
     3001 tggttcagga tacatctgta aaaattgaca gaggtattgt tgtagataag acattacaga
     3061 caaatgttaa agatatatat gcttgtggag atgttgcaca agtgggaaat attagtttag
     3121 caatatggcc atcttcagta gaaatgggaa aaatagcagg ggcaaatgca agtggagata
     3181 atttgacatt tgaaagtgaa gtatatccag tttcattaga tgcaatgaat gtaaaagtat
     3241 ttagtattgg taatatacaa aattttgata aagaaatttc atcaaaagat gaaggtcaaa
     3301 gaatatataa aaaattattt atgaaagatg gttcattagt tggagctata cttataaatg
     3361 atttaagctg tactgttaaa ttgataagat taatatctga aaaaggtgat tttgaggata
     3421 ttatgaaggc tgatatacta taatttaaat acattaaaat aaaaaggagt tgtcttaaaa
     3481 ttgatttttt tctaatcaaa ttttaaattc aactcctttt ttgaattttt atataaaata
     3541 acatggaaat tttagtaatg tctgtggtta catacatagg tttaattgtt ttttatatgg
     3601 attgtctctg gtataataaa ttgaattgtg tttgactgat agatattttt attgaaagtt
     3661 aatacctgtt atgcgataat aaggacgtgt taggggagtt tttataataa caattaatct
     3721 cagtttttgt gttccaatag tagcatcaac acattctgat gtagtagttc ttttcttata
     3781 aaaattagtt acaaatatgc ttgtaattat ttgatatgta aatttaccaa tattataaat
     3841 gatgacctcg ataataagga gagtgaaaaa taatctatga acgaaaatat tttgattgtg
     3901 gatgatgaaa aagaaatagc agatttaatt gaagtatatt taaaaaatga aggttttaca
     3961 gtatacaagt tttatacagg taaggaagct ctagaatgta ttgaatctaa aaatttggat
     4021 atggctattt tagatattat gcttcctgat attgatggat ttcatatttg ccagaaaatt
     4081 cgagaaaaat actattatcc tatcattatg ttaacagcta aaattgaaga tatagacaag
     4141 ataatgggtt taaccatagg tgctgatgat tatataacaa agccatttaa ccctttggaa
     4201 gttgttgcaa gagtaaagac acaattaaga agatatatgc gatataataa ttcttatgaa
     4261 caacagtcga taattgttaa tgagtatgat attaaaggat tgattatcaa taaagaaaca
     4321 cataaatgta gtttatttgg gaaagaagta gcattaacac cgattgaatt ttctattctt
     4381 tggtatttat gtgaacatca aggaaaagtg gttccttctg aagaattgtt tgaagctgta
     4441 tggggagaaa aatatcttga taataataac acagttatgg ctcatatagg gcgattacgt
     4501 gaaaaaattc atgaaccttc taaaaacccg aaatttataa aaactgtatg gggagtaggt
     4561 tatacaattg aaaagtaaaa atattaaagt caatcctgac tttactcaag tgagaaataa
     4621 aatttttatg agaacagctt taatggcatt tatagccatt attttcatat ggattttata
     4681 ttcttttata ttgtatggta agttttctat ctggatagta tcattctttg aaaatgcttt
     4741 tcgtatggaa tataaagctg cattgaattt atatcagcag attttccgta atagtatgga
     4801 gctcttcatg atactagcta tggtgattgt gttttttata atattccgtg tttatttaaa
     4861 ttggtttaca aaatatttta gtgaaataaa tcaaggtatt gactctctta tcaaagaaga
     4921 tgttggagaa gtggctttat ctccagagtt attggcaata gaaaagaaaa taaactcaat
     4981 taaacatata cttgaacaac gtaaatttga gacacaaatg gcagaacaac gtaagaatga
     5041 acttattgtt tatcttgcac atgatttaaa aacaccactt acatcagtta tagggtatct
     5101 gacactactt cgagatgaaa gtcagatttc tgaagaactg cgaaaaaaat atttatctat
     5161 ttcgttggat aaggcagagc gtcttgaaga tttaattaat gaattctttg aaattacaag
     5221 gtttaattta tcaaatatat cacttgaata cagtacagta agcttaactc gtatgcttga
     5281 acaactaaca tatgagttta aacctacttt gatgagtaag aatttaaaat atacactgaa
     5341 tgttacacca aatatgatga taaggtgtga tgttaataaa atgcaacgtg tatttgataa
     5401 cttattacga aatgctgttt attatagttt tgaagatact actattgaaa taacagctgt
     5461 acaggaggat gattatataa agcttaaatt tataaatcat agcaatacga ttccagagga
     5521 aaaacttgag cgtatttttg aacaatttta caggttggat acatctcgta attcaaacag
     5581 tggtagagct gggttgggac ttgctatagc taaagaaata gtggagcttc ataacgggac
     5641 aataacagct catagcgaga acaatataat tacctttaag gttattattc cgattgtagg
     5701 aaatttgtaa gaatttaagt ataaaaaaat aggaaattct ttaggataaa taaataaaga
     5761 gcactcttgt tattgatata attactgtat caaaacaaga gtgcttttat tttgatacag
     5821 taattatatc aatagaaaaa tgtgaaaata tattatgtgg aacagatgca attgtaagtg
     5881 aaggttgatt ggatttgaaa tagaaaaagt attttaacat gtttctgtag attgtataaa
     5941 aataaataaa tcaacaaagg agaatcaaag atgaataata aaaaaattgc agttatattt
     6001 ggtggaaact caacagaata tgatgtatca ttacaatcag cttttgcagt acttgagaat
     6061 ttgaatacgg aaaaatatga tattatacca attggaatta caagggaggg taattggtat
     6121 cattatattg gagattataa taatatccag aataacactt ggtttgaaag ggatactgat
     6181 ataattccag ttattgtttc gcagaaccgt gtcaaaaatg gtattattga atttttaatt
     6241 cctgatgctg tttctcagaa tcatattcca aatagtgcta ttgaatcatg tgaaaatgat
     6301 attaaataca ctaaacttga ttttgtgttt cctgtactgc atggaaaaaa tggagaagat
     6361 ggaacagtgc aggggttatt tgaattggca ggtatacagg ttattggatg tggtactctt
     6421 tcttcagcat tatgtatgga caaagacaaa tcacataaac ttgttcattc agaaggtatt
     6481 aaagtaccaa aatcaatatt actaaaatac tcagataaat ttaatgtggc tgaactgtca
     6541 aaagacttaa cttatccatt atttataaaa cctgtgcgtg caggttcatc atttggtatt
     6601 agtaaaattt ataaaaaaga agagttacaa gtagcagtag acttggcttt tgaacatgat
     6661 aatgaagtaa taattgagga aaatattgat ggttttgagg tagggtgtgc agttttagga
     6721 aacaatgact tacttgttgg aagagtagat gaaatagaat tgtcaaaagg attttttgat
     6781 tatacagaaa aatatacatt gaaaagttca aaaattcata tgccagctag aattgatttg
     6841 gattgtgaga aaagaataca agagacagca aaaattattt atagaactct tggatgttct
     6901 ggatttgcta gagtagatat gtttttaact ccatctggtg aaattatttt taatgaagtc
     6961 aatacaattc ctggctttac ttctcatagt cgttatccaa atatgatgaa aggtattgga
     7021 ctgtcttttg aagaaatatt ggacaagata attgaggtgt acataaaatg atgaataata
     7081 ttttattaga taaaaataaa gaagataaaa tgacaaatac tatcttattg actaaagata
     7141 aagtttatga tgggaatcta attctagtaa atgctttttt accagttaaa acatctgaag
     7201 atatagattt aattcctgta gatacaaggt ttccaagtat tcttatgaaa cgtgaagcta
     7261 caaacatttt acaaaatatt ttaaaaagta tatgtggaat aaatgaaata gtacctgtaa
     7321 gtggatatcg tactgcagag gaacaacaag atatatattc aagttctctg cgtgacaatg
     7381 gaaaagactt tactaaaaag tttgtagcac ttccaaatca cagtgaacat cagacagggc
     7441 ttgctattga tttagggtta aagaaagaag ttatagattt tatctgccct gaatttcctt
     7501 ataatggtat ttgtaatgag tttcgtaagg cagcactaca ttatggtttt atagagcgtt
     7561 atcagtgtaa aaaagaagaa ataacaggaa tatctcaaga accatggcat tttcgttatg
     7621 tgggatatcc gcattctgag attatcaata aaatgaatct cgctcttgaa gaatatatag
     7681 aatatattaa gagattttcg tatacaaatt gtttaaaaat agaagataaa gaaaaaatta
     7741 tagaaatctt ttatgtgcct attcatacta aagaagaagt gagaatctct atacctgaac
     7801 aatgtgtata tcaaatatcg ggtaacaata aggatggttt tattgtgaca ttttggagaa
     7861 atagaaagga ctaagaaatg gaggaacaag aatgattagg aataaatctt atgcagggct
     7921 tgattatttt aggattatag ctgcattact tataattgca attcatacat ctccattatt
     7981 atcatatagt aaaatggctg actttgtgtt aactagtata attgctcgta ttgctgtgcc
     8041 attctttttt atgctatcag gcttttttct aatttcaaca tataattata attgtaataa
     8101 actgaaatct tttctaaaaa atgtattatt tatttacggg atagctattg taatttatat
     8161 tcccttaaat atttacactg gatatttttc catgaataat ttagtgccca atattattaa
     8221 ggacattgta tttgatggga ctttatatca tttatggtat cttcctgcat caattattgg
     8281 tgcaagtatt gcatactttc ttgtaaaaaa actaggttta aagtactcac tagtaatcac
     8341 tattgttatt tatgtaattg gtatgtttgg tgatagttat tatggttttt tagaaaaaat
     8401 accactatta aagaatttat atgggtatat ttttgaagta tcagattata cacgaaatgg
     8461 tatatttttt gcacctgtat ttttcatact tggtggaata atatctaata aatctaatta
     8521 tatgtcaact aaaaatagct tgattggatt tcttatatca ttcttactta tgttatgtga
     8581 gggaatgttt ttacataaac taggggtaca gcgtcatgat agcatgtata ttatgttatt
     8641 gccatgtatg tatttcttat ttacagcctt aacatttttg aaaggttctc gagctaggtt
     8701 attgcgaaca tcatcattaa ttatttatat tatacatcct atgatgattg ttgttattcg
     8761 tatgttttct aaaatattcg gaatacaaac tgtcttggtt gacaatagcc ttattcatta
     8821 tatagtggta agtatagcat cttttatagc ttctataatt attatattga ttcttaaacc
     8881 tctaaaaaaa gacttgaaag gagaacataa ggtaaatacc aatagagctt ggattgaaat
     8941 tgatttaaat aatttggaac ataatgtaaa gatgatacaa aaagctatgc cagaagattg
     9001 tgaattgatg gcagttgtaa aagctaatgc ctatggtcat agtgcttttg aagtagcgac
     9061 ttgtgcaaac caaataggtg taaaagcatt tgcagtagca acaatagatg aggggattga
     9121 tttacgtcga tatggaatac taggagaaat attgattctt ggatatacta acccaataag
     9181 ggcaaaggaa cttcataaat acaatcttac acagagtgtt attgacctta atcatgctat
     9241 ctctttaaac aaacagaaat acaacataaa atcacacatt aaaattgata caggtatgca
     9301 cagactgggt tttgatgtta atgatgtgag gtcaatttta aaggcattta aattaaaata
     9361 cttagatata tgtggcatat ttacacactt gtgtgtttca gatagtctga aagatgaaga
     9421 tgtagacttt acaaataaac aaattgaatg tttttataag ttaattgatt ttctgttaaa
     9481 aaaaggtata aaaattccta aagtacatat tcaaagtagt tatggttttt ttaattatcc
     9541 caatcttaag tgtaattatg taagaattgg aattgcatta tacgggatat taagttttcc
     9601 aaatgatacg actaaattgc aacttgattt acgacctgtt ttatccttaa aatcacaagt
     9661 aattcttatt agaaaaatac aaaaagatga tagttttgga tatggaagag tctttattgc
     9721 aagcagagat agcgtaattg ctatattatc tattggctat gccgatggta tacctagaaa
     9781 tttatcctgt ggaaaaagtt acgtaataat aaatggttgt agagcagcaa tagtgggtcg
     9841 aatttgcatg gaccaacttg ctgttgatat tacagatata cctaatgtag aagttggtaa
     9901 tacggcaata ataataggaa gagataattt atctgaatta tctgtttctg aagttgcaaa
     9961 taactcatgt agtatatcga atgaattatt gagcagagta ggaagaaggt taaatgttat
    10021 aaagaagtaa ctcaattaaa tagtaatatt tgttgtactg tgtaatttat tttactctga
    10081 ctagacattt ttagtaatat tatgtctaat atatattttt tataacttac atgacaattt
    10141 aagttataaa aaggtgatgt ttcagatttt tattttgaaa catcaccttt tttgttataa
    10201 tatataataa aataattata ccttaactgt aaacttatat agattattta ctttttaata
    10261 ttagattcat tttttgagat tggtatataa taaacatata gagtatataa taatagttaa
    10321 agttaaaaaa ggggaaaagc aatatgcagt atatacaaga actacttgat ttgactcaaa
    10381 attattgggt gttagccaca ataatcggct tattaagtgc atttatagaa agctttatac
    10441 cagccttacc acttgttgct atagttacag caaatgctgc aatacaagga ttatttattg
    10501 gatgtttgtt atcatggatt ggctcaggac ttgggacaac ttcattattc ttattaataa
    10561 gcagatttaa tgatagtaag ctttttaata agttgagaaa tagtaaaact gaaaaagcaa
    10621 taagttggat ggataaacag ggatttaagt tattatttat agcgtatgcc tgtccattta
    10681 tgcccggttg tttagttact atcgcatcag cttttggtaa aaaagattta aaagattttg
    10741 tgccagcaat gttggcagga aaatttgtaa tgtttatagt tataagttac gtggcaagtg
    10801 atatagaagg ttttataaca agtcctttaa agatagcatc atttatatta ttggtgtttt
    10861 tgtcatggaa gattggaaat agagtaaata aaaatcttga aaatcataat tatgatttcc
    10921 atcataaaaa acatcacaat gataaagatg ataaaatgat ttagcttgta cgaaataaaa
    10981 cgtatcttaa aatgatttag tactaaatta ttttaagata cgttttattt tttgtattaa
    11041 ggtacaaaat ttttagttct tttgtaaact aaaatatata aaacttattt atacttagtt
    11101 caaaatattg ttatttattt tttctacaac tataataaaa tataaaattg atatccaatc
    11161 aaaaaaataa ctatgaaatt taatagctta gatatgaatt tttctgtttt tgagttagtt
    11221 tttataggta gtaaagaaat gtttatagta atgggataaa acaattcaat accttcttta
    11281 gtaataaggt caagacacaa gtgagataga tatcctatta taaacccact ggacaaacat
    11341 gagaaaacta tattattgtt agtgaagatt gtcagagatt taaaaaaact ataaataagt
    11401 aataaggcaa ttagactgtg agtaaaccct ctatgtctaa acctacttcc aaataatttg
    11461 tatattaaca taaatttttt acttatgtaa gaacctctca tatctatatc tggaaataaa
    11521 gaacctaaat atgcaaaata aatataaatt ataattaata ctattttata tgcaatatcg
    11581 tatttaacta ggtacagatt gtttataaag gggagtatca aaagtgcgaa tatataacct
    11641 cccctttcat gtgtttcttt tatcatataa aaatcctcct gaatcgtcac tataaacatt
    11701 atattggtaa atttagacta tcttttatta tattatatag caaacataag tttttttcaa
    11761 aaggatttta atatttaatt aatcttgaga ttttaaattt ttgtaatttt ctaaagcacc
    11821 attccaattt actacattaa accagttgtc aatgtattca tttcttctat tttgatattt
    11881 taaatagtaa gcatgctccc aaacatcaag tcctattata ggagttaggt ttttacttac
    11941 agggctatcc tgatttggag tagtcataat agataatttc ccatctttag tagctacaag
    12001 ccaagcccaa ccagaaccaa agacatctaa agcagatttt tggaactctt gcttaaattt
    12061 ttcaaaagaa ccaaagtctc tatcaatagc ttcttttaaa gattcagaag gtatggtttt
    12121 ttctggcgtc attatatcaa aaaagaattt atgattataa gctccacctg cattatttct
    12181 tacagttgta gcaatatctt taggtaaaga atctaaattt tgcaataatt cacataaaga
    12241 ataattataa agctcaggat atttttcaag agcagcattt aatttatcaa cataagcttg
    12301 ataatgctta tcatgatgca gtttcattgt ttctttatct atataaggtt caagtgcatc
    12361 atatgcataa ggtaatggtt taaccttaaa tttgttattt tcaggtgtaa aagcaaatga
    12421 agttatgcac tgtgagatta taaataaaga cataataact ggtattaata tttttttctt
    12481 cataaaaatc acctcctaat atagtatttc tagaaataaa aaaaatatga aatattaaca
    12541 gataatttat aagaatttaa tagcaatttc attttaaatt gaataagttt taaagccaaa
    12601 gacaaaacta tgaaatatag aataagacta aaggacagaa aggaattttg attatatgga
    12661 cggcaatatt tcaatttatt ataaaggtga tatatctaaa aaagaagata ttaaatttaa
    12721 aataaaagaa tctgtaaata atacaagact ttctagtaga atagagtata agga
//
LOCUS       DACULQ010000374         7170 bp    DNA     linear   BCT 11-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD08173
            SAMN10435664-rid8538883.guided.176, whole genome shotgun sequence.
ACCESSION   DACULQ010000374 DACULQ010000000
VERSION     DACULQ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10435664
            Sequence Read Archive: SRR8199414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000622069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000622069.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/01/2019 11:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 3,812
            CDSs (total)                      :: 3,722
            Genes (coding)                    :: 3,642
            CDSs (with protein)               :: 3,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 35 of 80
            Pseudo Genes (incomplete)         :: 37 of 80
            Pseudo Genes (internal stop)      :: 25 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10435664-rid8538883.guided.176"
                     /strain="CD08173"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2018"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            104..1057
                     /locus_tag="JA154_19035"
     CDS             104..1057
                     /locus_tag="JA154_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein transpeptidase
                     domain-containing protein"
                     /protein_id="HAT7997872.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1401..2084
                     /locus_tag="JA154_19040"
     CDS             1401..2084
                     /locus_tag="JA154_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT7997873.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2081..4741
                     /locus_tag="JA154_19045"
     CDS             2081..4741
                     /locus_tag="JA154_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT7997874.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(4922..6103)
                     /locus_tag="JA154_19050"
     CDS             complement(4922..6103)
                     /locus_tag="JA154_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT7997875.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6103..6765)
                     /locus_tag="JA154_19055"
     CDS             complement(6103..6765)
                     /locus_tag="JA154_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAT7997876.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(6897..>7170)
                     /locus_tag="JA154_19060"
     CDS             complement(6897..>7170)
                     /locus_tag="JA154_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT7997877.1"
                     /translation="DTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDIEIPTLM
                     YSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
BASE COUNT         2668 a          910 c         1135 g         2457 t
ORIGIN      
        1 gtcttgtttc ttataagtag aacctagatt ggagtatatt acaaataatg tctactattt
       61 taatagtagg ttataaattt aaatatggga gatgatataa attatgaaaa gaaaaaagaa
      121 ttttatttgg atagctatct tattggttgg tgtagttatg gtaatgtatt atgtagggaa
      181 aaaacataac gatataaatc aaaaaactga taagaattat ataaaatctg aattaaataa
      241 gtctgaatta aataaatcca aaattgaaaa caatgataaa aacaaaaaag aaaaaattaa
      301 tatggttgac tatagtgatt gttttgaagg tattagtgga ggagctattt tttataatac
      361 taaaaataaa gaatataata tatataacaa agaattaatt gaaacaagaa ggtcaccatg
      421 ttctactttt aaaatagttt caactttaat tggtttagaa aaaggggtaa taaactcaaa
      481 agaatcagtt atgggatatg atggtacaga gtatccaaat aaaaattgga ataaaaatct
      541 tagtttagaa gaggcattta aggagtcttg tgtttggtat tacaaaaaat tgatagataa
      601 agttgatgct aaatctgtcc aaaacatttt agatgattta aagtatggga attgcgatat
      661 tagcgaatgg gaaggcgatt taaaaaatgg aaagggacat ttaaatggat tttggttaga
      721 atcttcatta caaatatctc caaaagaaca agtgcaaaca atggcaaaaa tatttgaggg
      781 agatacaaac tttaaaaaag agcatattaa tattttaaga gatattatga aaatagatat
      841 aaatgataaa aacataaatg tatatggaaa aactggaaca ggatttgatg aaaaaaataa
      901 acgtgtggat gcatggtttg ttggtatgct agagcgtgaa ggtgacacct attattttgc
      961 tattaaatca gatgattcca ataaagaaat tacagggcca aaggtaaagg aaattgcaat
     1021 taatataatt aagaaatatt atagtgtaag ggaataaact taattttatt tgtaaatatt
     1081 atttacctat agtctgtaat atttattaaa taattttact aaattgagtc tatatagagc
     1141 aaaactcctt tagtacataa aatacttgct attgattatt aaattgaata cctatagatt
     1201 taaagttaat accttttaaa gactatgtaa tatattaaaa gtataaataa aatatattgc
     1261 atagtttttg ttttatatta taattttaaa taatgtgcaa aaactaaaca atatttttaa
     1321 tagtaattta tatttgtatt catgtcacta taatgtcatt ttaggttgct aaaatttaac
     1381 cataaaggag atgattgtat atggaaatat taaaatgtga aaatctcaca aaaatatatg
     1441 gttcaaatca aaccagagtg acagccctta ataatgtaaa tttatcagtt caaaaaggtg
     1501 attttgtatc cattgtagga gcatctggtt ctggcaaatc tacattgtta catctgcttg
     1561 ggggagtaga tagacctaca tctggaaaga tttatgttga agatacagaa atatcaagtt
     1621 taaaggaaga agctcttgca gtttttagac gtagaaaagt aggtcttatt tatcaatttt
     1681 ataatcttat acctaccctt gatgttagaa aaaatatatt attacccatg ttacttgata
     1741 aaagaaaggt ggatgaggat agattctctg agattgtatc tatattggga ttatctgata
     1801 gattaaacca tcttccaagt caactttcag gtggacaaca acaaagggta tctatagcta
     1861 gaagtttaat ctatagacca gctattttat tagcagatga gccaactggt aatcttgata
     1921 gaaaaaattc agaagaaatt gtagacttgt taaacttatc aaataaacga tttaatcaga
     1981 caatacttct tattactcat gatgagaaaa tagcactaga ggcaaatcgt attgtaacta
     2041 tggaagatgg agtgattgtc tctgaaaagg tggtgaagaa atgaatattc ttcaagaata
     2101 caccttagac tttgtacgac gcaataaacg tagtagtata gctataatgt tagctatatt
     2161 acttactaca accatgatgt cttctttgtg tggactgttg tataccatgt ggacagattt
     2221 tataagactt tcaattgaga aagaaggtaa ctggcatggt gaattgtttg actatactta
     2281 tggaagtgac ttaccattaa ttgaaaattt ttcttcagtt gatgatgtaa tggtaaaagg
     2341 acaatggtat gtaggcaaaa ttaatgatga gaaaagagat tatataattt atagaaatgc
     2401 tacatcagag tattggaatt ctatgccaga aaaggatact ataatagagg gtcgtcgacc
     2461 aaataatgct aatgaaattg cattatcaaa acaatatttt gagaataatc caagtgtaaa
     2521 aattggtgat aaaattactc tcccaatagg atatagaatc ttaaatggaa agcctcttga
     2581 acccatagcc ccaaaatcga aaggggaaag ttttaaaaaa atttcagagg ctacattgac
     2641 aatagtagga aaggtagatg tttctacttc atcagtaata cctgcatata cagctcttgg
     2701 atatcttgat aaaacaagta taaaacctaa tgatagtata actgtgtact tgcgatttaa
     2761 aaatataaga gacacgtata aggaacttcc aaaacttgct aaatcattgg gatggaagac
     2821 taatgaatat ggaaaataca atcttaaata caatagtaat tatcttttaa agatgttagt
     2881 cttttcacca gaacagaaag catctatgag tagtatatca aaattttcaa ctcctattat
     2941 gtatcttaca atagcaattt ttacagtagc cgtgtttgta atggtgattt acaacgcgtt
     3001 ttcactgtct gcaaatgcta gacttactca gttagggata ttatctagtg ttggtgcatc
     3061 accaaaacaa attaaaaggt ctgtagtgtt tgaaggcttt ttactgacaa ttataccatt
     3121 accaataggt ttatttttag gatggttatt gtgtaatagg cttatagttt atgttaattc
     3181 agttaattat catactgatg ttccagaagt ggtatttact tatggtattc ctgcttttct
     3241 gccagcagtt ttacttacaa tagtaacagt ctggatttct gcacttatac cagcacgaaa
     3301 agtatcaaag atatctccta tagaagcaat acgacaagga gatagtgtta aaatcaaaaa
     3361 gtcgcataaa tattcattag gaaagatatt tggaatagaa ggagaacttg catcaaacgc
     3421 actacgtgct cgtaaaaaat cttatcgtac tgcaacaata tctttaacat tatctttttt
     3481 attacttaca tgttttttgc atataattgc aaatcaagaa ggagcaaaag ctgtttatgg
     3541 aagtgataat tataaggacc aaagagatat agcagtttat ttggaaaata gtgaacctat
     3601 agagtctgct ttcattgaaa aattagataa tacagaaggt gtaaaaagtt cactatatta
     3661 tcaaaaaatg tctgcttcca cttggctgac tgaaaaagat gcatcaaaag aacttgcgaa
     3721 aaatggagga tttaaaagta tagtttcatc taaaaaatac tctcctatag aacgtgatgg
     3781 caagtttcgt ataatttcaa atataattgg gcttgatgat aagagtttta cagaatattg
     3841 taggcaaatt tctgtagacc caaagttatt ttataataca aaaaatccaa cctcaattgt
     3901 atataataag gaggaagata ttaatcgtag tacacgtaga aataaagtat atatagatta
     3961 tttaaatctt tcaataggag ataacattaa attaaatgaa aaagtatatg acgaagataa
     4021 aggggattat acttacaaca tgaaagtggg agctattaca agcacacttc ctaaaatagt
     4081 agatactagt tcaaagtata cattaatgca aatagttcca atgtctatgg tacaggaaat
     4141 atctaaaaat tatgatgaag taagtaggtt acgttcaaat aggctgatag gagtatttaa
     4201 agtaaataat cgtgaggaca ttcctagagt aagagaaaaa ttagagtcca tttataataa
     4261 agactatggt tcaggagatt atagtattga ggatgtttta gaaagtgaaa aacaaaaggc
     4321 tagtggaata aagaccatga atttaatagt aggctttatt acaggtttat tggctatgat
     4381 tggattatca aatgtacttg ctacagtgtc aggaaatatt agaagtagac gacaagagtt
     4441 tgctatgttg cgttctgttg gattatctcc agaaggcata aaaaagatgc ttattttgga
     4501 aggtttattc ttaggaatta caccattact acttagtatt cctgtgcaaa taggaatagt
     4561 gtatgctttt ctacgtataa atgaaatata ttttatagag tatttaccat ttgcacctat
     4621 atctacaata ataggtttta ctatattgat actttttatt gtaatagcat catatatgac
     4681 aggatacaag cagttgaaga atgaaaatat agtagaatct ataaaaaatg aaacaatata
     4741 aacaaaatct atttgaactt atttaaaggt ttcgatttaa gactacttac aagtataggg
     4801 atagttaagt tgtttgttta ataaaacctt aaatgtaaat agaagtatat tactaaaaca
     4861 atagataaca tagaatatag atatagctgg attataggta ttttacctat aatccagcat
     4921 tttatgtgta aaatttaatt atgaatcttg caccaccatt atggcaattt tctgcatgta
     4981 gagtaccatt ttgacgttct ataatagact ttgatagagc aagccctata ccaatactgt
     5041 ccttactagc atttttacca cgataaaatc tattaaaaat atatggaagt tcttcctgag
     5101 caaacccctt accattatct tctatgataa tctcaacata tagaggattt tgactataat
     5161 aagcaactat atatccacca tcttctatat gctcactaca atttttaaga atatttaaaa
     5221 gagcttctaa agtccaatta atatcgattt caaattctac cgaaggagaa gcctcgatag
     5281 taaatgtctg atgtttttct cttaaagaac cttctatagg ctcaattgca taagtaagaa
     5341 gagaatgtac atctataagt ttgctttcaa atattaaagt acttgcatcc aattttgata
     5401 atgtaagtaa agatgaaact agtctctcaa gtcttgagat ttgatttgta agtctgttaa
     5461 tataatctat ttcttcatct ggacaatttt ctgaaagtaa ttgtgccata agtgacatag
     5521 aagtaattgg agtcttaagt tgatgagcaa tatctgcaag attatctgaa agagtttgtc
     5581 tttcttttaa agcaaattct tttgaacaac gaagttcccc aactgtttta tatatctcat
     5641 cttcaaggtg agaaaattca tcttcacatc gtaaaagtac agactctttt cctaaattca
     5701 cttcttctaa gtattgagta agattgttta ttctactcag tttttgctta tttgttctat
     5761 gtttgattat atatataact gtagccattg ttgaaattat accaaacgat atcactagaa
     5821 aataaaaaaa gtttttactc caaaatgtac ttttagaata tccatgtttt gataaaattt
     5881 catttcctgt tttaaaatcc ttatcactag aatttttcag tgattttagt atactttctg
     5941 tagacttagg attgtactca tatgatgcac caagagcctt agacagtaca ttgtattcat
     6001 taaaagaagt tatagttgtt agaattacac tcaataaaaa agttacaatt aagcagaata
     6061 ttataaaaac aactgtactg ttgatatttc ttggagtttt cactagctgt cctccattct
     6121 atatcctatg cctcttaacg tttttataca aggtggattt tttaattttt ctctaagacg
     6181 ttttattgta acagtaagag tgttatcatt tacgaaatta ccatctacat cccagagcat
     6241 ttctagtaac tttgtacgag tcatagctct atttttattt tccataagac ttagaagaag
     6301 tttatattct cctgctgtca aaggtacttc aacattatct aaatatgctt tgacttcaac
     6361 cttattcaag cttatatttt cacatgtaat agtattgtta gtatttgaat gagttcttct
     6421 aagaactgca ttaattctgg actgaagtac tgagagcaaa aacggtttaa ccacatagtc
     6481 atctgcaccc atatctaatc cacgtataat gtctttttca tcatcacgga ctgtcaaaaa
     6541 tatcactgga gtatcttcta tacttttaac atattggcaa aactcgtatc caataccatc
     6601 aggtagattt aaatcaagca gaattaaatc aatagtttta tcaaaatttt ttttagcttc
     6661 agcaatcgta ttataacaac atacagtgtg atttttcttt tcaagggaag tttttattcc
     6721 aaatgtaatt gtatcatcat cttctaaaag aagtatttta gccatttact tttaatcctt
     6781 tcagtaattt agttattcca ttaactaagt tattagtttt atgtttaata ttttttatat
     6841 tagagataga aaaaattatc atttaagtat ataaacttta atttataaac ttaggtttat
     6901 gctttaattg aatctattaa aagagcaaga gttgtttcaa taaatttttc ctcatctata
     6961 ttaaagttgt tcaggtatga ttttcctgtt acagatagta aattaaaaaa tcctgtaagt
     7021 gtaaatattg atgaatacat aagagtaggt atttcaatat catgcctaat acttctatca
     7081 ttttttccaa tttcaaacat tccttgtatt tcatcaaaca taaatttatt aaaagaaaaa
     7141 aacttttctt taaacagcat atcagtatcc
//
